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  1. Spectral characteristics of the mutant form GGBP/H152C of D-glucose/D-galactose-binding protein labeled with fluorescent dye BADAN: influence of external factors

    PubMed Central

    Fonin, Alexander V.; Stepanenko, Olga V.; Povarova, Olga I.; Volova, Catherine A.; Philippova, Elizaveta M.; Bublikov, Grigory S.; Kuznetsova, Irina M.; Demchenko, Alexander P.

    2014-01-01

    The mutant form GGBP/H152C of the D-glucose/D-galactose-binding protein with the solvatochromic dye BADAN linked to cysteine residue Cys 152 can be used as a potential base for a sensitive element of glucose biosensor system. We investigated the influence of various external factors on the physical-chemical properties of GGBP/H152C-BADAN and its complex with glucose. The high affinity (Kd = 8.5 µM) and high binding rate of glucose make GGBP/H152C-BADAN a good candidate to determine the sugar content in biological fluids extracted using transdermal techniques. It was shown that changes in the ionic strength and pH of solution within the physiological range did not have a significant influence on the fluorescent characteristics of GGBP/H152C-BADAN. The mutant form GGBP/H152C has relatively low resistance to denaturation action of GdnHCl and urea. This result emphasizes the need to find more stable proteins for the creation of a sensitive element for a glucose biosensor system. PMID:24711960

  2. Fluorescence probe study of Ca2+-dependent interactions of calmodulin with calmodulin-binding peptides of the ryanodine receptor.

    PubMed

    Gangopadhyay, Jaya Pal; Grabarek, Zenon; Ikemoto, Noriaki

    2004-10-22

    We have used a highly environment-sensitive fluorescent probe 6-bromoacetyl-2-dimethylaminonaphthalene (badan) to study the interaction between calmodulin (CaM) and a CaM-binding peptide of the ryanodine receptor (CaMBP) and its sub-fragments F1 and F4. Badan was attached to the Thr34Cys mutant of CaM (CaM-badan). Ca(2+) increase in a physiological range of Ca(2+) (0.1-2 microM) produced about 40 times increase in the badan fluorescence. Upon binding to CaMBP, the badan fluorescence of apo-CaM showed a small increase at a slow rate; whereas that of Ca-CaM showed a large decrease at a very fast rate. Upon binding of CaM to the badan-labeled CaMBP, the badan fluorescence showed a small and slow increase at low Ca(2+), and a large and fast increase at high Ca(2+). Thus, the badan probe attached to CaM Cys(34) can be used to monitor conformational changes occurring not only in CaM, but also those in the CaM-CaMBP interface. Based on our results we propose that both the interaction interface and the global conformation of the CaM-CaMBP complex are altered by calcium.

  3. Business Ethics

    DTIC Science & Technology

    2002-06-01

    multimedialna,” 2001 17. Public Opinion Research Center, “ Korupcja i afery korupcyjne w Polsce,” Warszawa, August 2001 18. Public Procurement...Law,” December 17, 2001, http://www.llrx.com/features/polish.htm 20. Supreme Audit Chamber, “Zagrozenie korupcja w swietle badan NIK,” Warszawa

  4. Fluorescence-based sensing of glucose using engineered glucose/galactose-binding protein: A comparison of fluorescence resonance energy transfer and environmentally sensitive dye labelling strategies

    SciTech Connect

    Khan, Faaizah; Gnudi, Luigi; Pickup, John C.

    2008-01-04

    Fluorescence-based glucose sensors using glucose-binding protein (GBP) as the receptor have employed fluorescence resonance energy transfer (FRET) and environmentally sensitive dyes, but with widely varying sensitivity. We therefore compared signal changes in (a) a FRET system constructed by transglutaminase-mediated N-terminal attachment of Alexa Fluor 488/555 as donor and QSY 7 as acceptor at Cys 152 or 182 mutations with (b) GBP labelled with the environmentally sensitive dye badan at C152 or 182. Both FRET systems had a small maximal fluorescence change at saturating glucose (7% and 16%), badan attached at C152 was associated with a 300% maximal fluorescence increase with glucose, though with badan at C182 there was no change. We conclude that glucose sensing based on GBP and FRET does not produce a larger enough signal change for clinical use; both the nature of the environmentally sensitive dye and its site of conjugation seem important for maximum signal change; badan-GBP152C has a large glucose-induced fluorescence change, suitable for development as a glucose sensor.

  5. The trehalose/maltose-binding protein as the sensitive element of a glucose biosensor

    NASA Astrophysics Data System (ADS)

    Fonin, A. V.; Povarova, O. I.; Staiano, M.; D'Auria, S.; Turoverov, K. K.; Kuznetsova, I. M.

    2014-08-01

    The promising direction of the development of a modern glucometer is the construction of sensing element on the basis of stained (dyed) protein which changes its fluorescence upon glucose binding. One of the proteins that can be used for this purpose is the D-trehalose/D-maltose-binding protein (TMBP) from the thermophilic bacteria Thermococcus litoralis. We investigated the physical-chemical properties of the protein and evaluated its stability to the denaturing action of GdnHCl and heating. It was confirmed that TMBP is an extremely stable protein. In vivo, the intrinsic ligands of TMBP are trehalose and maltose, but TMBP can also bind glucose. The dissociation constant of the TMBP-glucose complex is in the range of 3-8 mM. The binding of glucose does not noticeably change the intrinsic fluorescence of the TMBP. To register protein-glucose binding, we used the fluorescence of the thiol-reactive dye BADAN attached to TMBP. Because the fluorescence of BADAN attached to the cysteine Cys182 of TMBP does not change upon glucose binding, the mutant forms ТМВР/C182S/X_Cys were created. In these mutant proteins, Cys182 is replaced by Ser, removing intrinsic binding site of BADAN and a new dye binding sites were introduced. The largest increase (by 1.4 times) in the intensity of the dye fluorescence was observed upon TMBP/C182S/A14C-BADAN-Glc complex formation. The dissociation constant of this complex is 3.4 ± 0.1 mM. We consider TMBP/C182S/A14C mutant form with attached fluorescent dye BADAN as a good basis for further research aimed to develop of series of TMBP mutant forms with different affinities to glucose labeled with fluorescent dyes.

  6. The different influence of the residual layer on the development of the summer convective boundary layer in two deserts in northwest China

    NASA Astrophysics Data System (ADS)

    Lin, Zhao; Bo, Han; Shihua, Lv; Lijuan, Wen; Xianhong, Meng; Zhaoguo, Li

    2016-12-01

    The development of the atmospheric boundary layer is closely connected with the exchange of momentum, heat, and mass near the Earth's surface, especially for a convective boundary layer (CBL). Besides being modulated by the buoyancy flux near the Earth's surface, some studies point out that a neutrally stratified residual layer is also crucial for the appearance of a deep CBL. To verify the importance of the residual layer, the CBLs over two deserts in northwest China (Badan Jaran and Taklimakan) were investigated. The summer CBL mean depth over the Taklimakan Desert is shallower than that over the Badan Jaran Desert, even when the sensible heat flux of the former is stronger. Meanwhile, the climatological mean residual layer in the Badan Jaran Desert is much deeper and neutrally stratified in summer. Moreover, we found a significant and negative correlation between the lapse rate of the residual layer and the CBL depth over the Badan Jaran Desert. The different lapse rates of the residual layer in the two regions are partly connected with the advection heating from large-scale atmospheric circulation. The advection heating tends to reduce the temperature difference in the 700 to 500-hPa layer over the Badan Jaran Desert, and it increases the stability in the same atmospheric layer over the Taklimakan Desert. The advection due to climatological mean atmospheric circulation is more effective at modulating the lapse rate of the residual layer than from varied circulation. Also, the interannual variation of planetary boundary layer (PBL) height over two deserts was found to covary with the wave train.

  7. Mechanism of interactions of α-naphthoflavone with cytochrome P450 3A4 explored with an engineered enzyme bearing a fluorescent probe†

    PubMed Central

    Tsalkova, Tamara N.; Davydova, Nadezhda Y.; Halpert, James R.; Davydov, Dmitri R.

    2008-01-01

    Design of a partially cysteine-depleted C98S/C239S/C377S/C468A cytochrome P450 3A4 mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive fluorescent probes into α-helix A‥ The site of modification was identified as Cys-64 with the help of CYP3A4(C58) and CYP3A4(C64), each bearing only one accessible cysteine. Changes in the fluorescence of CYP3A4(C58,C64) labeled with 6-bromoacetyl-2-dimethylaminonaphthalene (BADAN), 7-diethylamino-3-(4’-maleimidylphenyl)-4-methylcoumarin (CPM), or monobromobimane (mBBr) were used to study the interactions with bromocriptine (BCT), 1-pyrenebutanol (1-PB), testosterone (TST), and α-naphthoflavone (ANF). Of these substrates only ANF has a specific effect, causing a considerable decrease in fluorescence intensity of BADAN and CPM and increasing the fluorescence of mBBr. This ANF-binding event in the case of BADAN-modified enzyme is characterized by an S50 of 18.2 ± 0.7, compared with the value of 2.2 ± 0.3 for the ANF-induced spin transition, thus revealing an additional low affinity binding site. Studies of the effect of TST, 1-PB, and BCT on the interactions of ANF monitored by changes in fluorescence of CYP3A4(C58,C64)-BADAN or by the ANF-induced spin transition revealed no competition by these substrates. Investigation of the kinetics of fluorescence increase upon H2O2-dependent heme depletion suggests that labeled CYP3A4(C58,C64) is represented by two conformers, one of which has the fluorescence of the BADAN and CPM labels completely quenched, presumably by photoinduced electron transfer from the neighboring Trp-72 and/or Tyr-68 residues. The binding of ANF to the newly discovered binding site appears to affect the interactions of the label with the above residue(s), thus modulating the fraction of the fluorescent conformer. PMID:17198380

  8. Analysis of variables affecting unemployment rate and detecting for cluster in West Java, Central Java, and East Java in 2012

    NASA Astrophysics Data System (ADS)

    Samuel, Putra A.; Widyaningsih, Yekti; Lestari, Dian

    2016-02-01

    The objective of this study is modeling the Unemployment Rate (UR) in West Java, Central Java, and East Java, with rate of disease, infant mortality rate, educational level, population size, proportion of married people, and GDRP as the explanatory variables. Spatial factors are also considered in the modeling since the closer the distance, the higher the correlation. This study uses the secondary data from BPS (Badan Pusat Statistik). The data will be analyzed using Moran I test, to obtain the information about spatial dependence, and using Spatial Autoregressive modeling to obtain the information, which variables are significant affecting UR and how great the influence of the spatial factors. The result is, variables proportion of married people, rate of disease, and population size are related significantly to UR. In all three regions, the Hotspot of unemployed will also be detected districts/cities using Spatial Scan Statistics Method. The results are 22 districts/cities as a regional group with the highest unemployed (Most likely cluster) in the study area; 2 districts/cities as a regional group with the highest unemployed in West Java; 1 district/city as a regional groups with the highest unemployed in Central Java; 15 districts/cities as a regional group with the highest unemployed in East Java.

  9. Estimation of unemployment rates using small area estimation model by combining time series and cross-sectional data

    NASA Astrophysics Data System (ADS)

    Muchlisoh, Siti; Kurnia, Anang; Notodiputro, Khairil Anwar; Mangku, I. Wayan

    2016-02-01

    Labor force surveys conducted over time by the rotating panel design have been carried out in many countries, including Indonesia. Labor force survey in Indonesia is regularly conducted by Statistics Indonesia (Badan Pusat Statistik-BPS) and has been known as the National Labor Force Survey (Sakernas). The main purpose of Sakernas is to obtain information about unemployment rates and its changes over time. Sakernas is a quarterly survey. The quarterly survey is designed only for estimating the parameters at the provincial level. The quarterly unemployment rate published by BPS (official statistics) is calculated based on only cross-sectional methods, despite the fact that the data is collected under rotating panel design. The study purpose to estimate a quarterly unemployment rate at the district level used small area estimation (SAE) model by combining time series and cross-sectional data. The study focused on the application and comparison between the Rao-Yu model and dynamic model in context estimating the unemployment rate based on a rotating panel survey. The goodness of fit of both models was almost similar. Both models produced an almost similar estimation and better than direct estimation, but the dynamic model was more capable than the Rao-Yu model to capture a heterogeneity across area, although it was reduced over time.

  10. Acceleration of Topographic Map Production Using Semi-Automatic DTM from Dsm Radar Data

    NASA Astrophysics Data System (ADS)

    Rizaldy, Aldino; Mayasari, Ratna

    2016-06-01

    Badan Informasi Geospasial (BIG) is government institution in Indonesia which is responsible to provide Topographic Map at several map scale. For medium map scale, e.g. 1:25.000 or 1:50.000, DSM from Radar data is very good solution since Radar is able to penetrate cloud that usually covering tropical area in Indonesia. DSM Radar is produced using Radargrammetry and Interferrometry technique. The conventional method of DTM production is using "stereo-mate", the stereo image created from DSM Radar and ORRI (Ortho Rectified Radar Image), and human operator will digitizing masspoint and breakline manually using digital stereoplotter workstation. This technique is accurate but very costly and time consuming, also needs large resource of human operator. Since DSMs are already generated, it is possible to filter DSM to DTM using several techniques. This paper will study the possibility of DSM to DTM filtering using technique that usually used in point cloud LIDAR filtering. Accuracy of this method will also be calculated using enough numbers of check points. If the accuracy meets the requirement, this method is very potential to accelerate the production of Topographic Map in Indonesia.

  11. Strategic of Applying Free Chemical Usage In Purified Water System For Pharmaceutical Industry Toward CPOB (Cara Pembuatan Obat yang Baik) Indonesia To Reducing Environmental Pollution

    NASA Astrophysics Data System (ADS)

    Kartono, R.; Basuki, Y. T.

    2014-03-01

    The purpose of this paper is to examine the sets of model and literature review to prove that strategy of applying free chemical usage in purified water system for pharmaceutical industry would be help the existing and new pharmaceutical companies to comply with part of Natioanal Agency of Drug and Food Control / Badan Pengawas Obat dan Makanan (NADFC/BPOM) regulation in order to achieve "Cara Pembuatan Obat yang Baik" (CPOB) of Indonesia pharmaceutical industry. One of the main reasons is when we figured out the number of Indonesian pharmaceutical industries in 2012 are kept reducing compare to the increasing numbers of Indonesian population growth. This strategy concept also might help the industries to reducing environmental pollution, and operational cost in pharmaceutical industries, by reducing of the chemical usage for water treatment process in floculation and cougulation and chlorination for sterillization. This new model is free usage of chemicals for purified water generation system process and sterilization. The concept offering of using membrane technology- Reverse Osmosis (RO) membrane base treatment to replace traditional chemical base treatment, following enhance Electrodeionization (EDI) as final polisher for controlling conductivity, and finally Ultra Violet (UV) disinfectant technology as final guard for bacteria controls instead of chemical base system in purified water generation system.

  12. Performance Measurement using KPKU- BUMN in X School Education Foundation

    NASA Astrophysics Data System (ADS)

    Arijanto, Sugih; Harsono, Ambar; Taroepratjeka, Harsono

    2016-01-01

    The purpose of this research is to determine X School's Strengths and Opportunity of Improvement through performance measurement using KPKU-BUMN (Kriteria Penilaian Kinerja Unggul - Kementerian Badan Usaha Milik Negara). KPKU-BUMN is developed based on Malcolm Baldrige Criteria for Performance Excellent (MBCfPE). X school is an education foundation at Bandung that has provides education from kindergarten, elementary school, to junior and senior high school. The measurement is implemented by two aspects, Process and Result. The Process is measured by A-D-L-I approaches (Approach- Deployment-Learning- Integration), on the other hand The Result is measured by Le-T-C-I approach (Level-Trend- Comparison-Integration). There are six processes that will be measured: (1) Leadership, (2) Strategic Planning, (3) Customer Focus, (4) Measurement, Analysis and Knowledge Management, (5) Work Force Focus, and (6) Operation Focus. Meanwhile, the result are (a) product & process outcomes, (b) customer-focused outcomes, (c) workforce-focused outcomes, (d) leadership & governance outcomes, and (e) financial & market outcomes. The overall score for X School is 284/1000, which means X School is at “early result” level at “poor” global image.

  13. Rheostat re-wired: alternative hypotheses for the control of thioredoxin reduction potentials.

    PubMed

    Bewley, Kathryn D; Dey, Mishtu; Bjork, Rebekah E; Mitra, Sangha; Chobot, Sarah E; Drennan, Catherine L; Elliott, Sean J

    2015-01-01

    Thioredoxins are small soluble proteins that contain a redox-active disulfide (CXXC). These disulfides are tuned to oxidizing or reducing potentials depending on the function of the thioredoxin within the cell. The mechanism by which the potential is tuned has been controversial, with two main hypotheses: first, that redox potential (Em) is specifically governed by a molecular 'rheostat'-the XX amino acids, which influence the Cys pKa values, and thereby, Em; and second, the overall thermodynamics of protein folding stability regulates the potential. Here, we use protein film voltammetry (PFV) to measure the pH dependence of the redox potentials of a series of wild-type and mutant archaeal Trxs, PFV and glutathionine-equilibrium to corroborate the measured potentials, the fluorescence probe BADAN to measure pKa values, guanidinium-based denaturation to measure protein unfolding, and X-ray crystallography to provide a structural basis for our functional analyses. We find that when these archaeal thioredoxins are probed directly using PFV, both the high and low potential thioredoxins display consistent 2H+:2e- coupling over a physiological pH range, in conflict with the conventional 'rheostat' model. Instead, folding measurements reveals an excellent correlation to reduction potentials, supporting the second hypothesis and revealing the molecular mechanism of reduction potential control in the ubiquitous Trx family.

  14. Vertical deformation at western part of Sumatra

    SciTech Connect

    Febriyani, Caroline Prijatna, Kosasih Meilano, Irwan

    2015-04-24

    This research tries to make advancement in GPS signal processing to estimate the interseismic vertical deformation field at western part of Sumatra Island. The data derived by Continuous Global Positioning System (CGPS) from Badan Informasi Geospasial (BIG) between 2010 and 2012. GPS Analyze at Massachusetts Institute of Technology (GAMIT) software and Global Kalman Filter (GLOBK) software are used to process the GPS signal to estimate the vertical velocities of the CGPS station. In order to minimize noise due to atmospheric delay, Vienna Mapping Function 1 (VMF1) is used as atmospheric parameter model and include daily IONEX file provided by the Center for Orbit Determination in Europe (CODE) as well. It improves GAMIT daily position accuracy up to 0.8 mm. In a second step of processing, the GLOBK is used in order to estimate site positions and velocities in the ITRF08 reference frame. The result shows that the uncertainties of estimated displacement velocity at all CGPS stations are smaller than 1.5 mm/yr. The subsided deformation patterns are seen at the northern and southern part of west Sumatra. The vertical deformation at northern part of west Sumatra indicates postseismic phase associated with the 2010 and 2012 Northern Sumatra earthquakes and also the long-term postseismic associated with the 2004 and 2005 Northern Sumatra earthquakes. The uplifted deformation patterns are seen from Bukit Tinggi to Seblat which indicate a long-term interseismic phase after the 2007 Bengkulu earthquake and 2010 Mentawai earthquake. GANO station shows a subsidence at rate 12.25 mm/yr, indicating the overriding Indo-Australia Plate which is dragged down by the subducting Southeast Asian Plate.

  15. Nested generalized linear mixed model with ordinal response: Simulation and application on poverty data in Java Island

    NASA Astrophysics Data System (ADS)

    Widyaningsih, Yekti; Saefuddin, Asep; Notodiputro, Khairil A.; Wigena, Aji H.

    2012-05-01

    The objective of this research is to build a nested generalized linear mixed model using an ordinal response variable with some covariates. There are three main jobs in this paper, i.e. parameters estimation procedure, simulation, and implementation of the model for the real data. At the part of parameters estimation procedure, concepts of threshold, nested random effect, and computational algorithm are described. The simulations data are built for 3 conditions to know the effect of different parameter values of random effect distributions. The last job is the implementation of the model for the data about poverty in 9 districts of Java Island. The districts are Kuningan, Karawang, and Majalengka chose randomly in West Java; Temanggung, Boyolali, and Cilacap from Central Java; and Blitar, Ngawi, and Jember from East Java. The covariates in this model are province, number of bad nutrition cases, number of farmer families, and number of health personnel. In this modeling, all covariates are grouped as ordinal scale. Unit observation in this research is sub-district (kecamatan) nested in district, and districts (kabupaten) are nested in province. For the result of simulation, ARB (Absolute Relative Bias) and RRMSE (Relative Root of mean square errors) scale is used. They show that prov parameters have the highest bias, but more stable RRMSE in all conditions. The simulation design needs to be improved by adding other condition, such as higher correlation between covariates. Furthermore, as the result of the model implementation for the data, only number of farmer family and number of medical personnel have significant contributions to the level of poverty in Central Java and East Java province, and only district 2 (Karawang) of province 1 (West Java) has different random effect from the others. The source of the data is PODES (Potensi Desa) 2008 from BPS (Badan Pusat Statistik).

  16. Oceanography of the Eastern Pacific—an introduction to volume III

    NASA Astrophysics Data System (ADS)

    Färber-Lorda, Jaime

    2004-03-01

    In 1998, the Centro de Investigación Cientı´fica y de Educación Superior (CICESE) celebrated its 25th anniversary, which was the occasion to start the series of colloquia that take place every 2 years, the Coloquio Sobre la Oceanografı´a del Pacifico Oriental, in Ensenada. Two volumes were published in 2000 (Färber-Lorda, 2000) and in 2002 (Färber-Lorda, 2002), with some of the papers presented during the events. CICESE supported the edition of the first two volumes, as well as the meetings. The papers of this present volume were presented during the III Coloquio Sobre la Oceanografı´a del Pacifico Oriental. A special volume concerning different aspects of the consequences in México of the 1997-1998 El Niño/La Niña 1998-1999, was recently published in México (Badan et al., 2003); however, it is not restricted to the Pacific nor only to oceanography. The Eastern Pacific encompasses a very wide range of different biotic and abiotic conditions. Papers on topics from Alaska, to the Chilean Fjords of southern Chile, and from the Gulf of California to the 180° meridian, around the equator, are presented (see Fig. 1). Even if unity in the subjects is impossible to achieve, the information is important to assess the research performed in the area. Here, we give a brief description of the papers presented. Some general discussion is possible and some common features of phenomena like El Niño and La Niña, and the 1998/1999 regime-shift of the North Pacific, are exposed. For the first time a special volume is dedicated to this area, instead of a particular topic.

  17. Exposure assessment of radon in the drinking water supplies: a descriptive study in Palestine

    PubMed Central

    2012-01-01

    Background Radon gas is considered as a main risk factor for lung cancer and found naturally in rock, soil, and water. The objective of this study was to determine the radon level in the drinking water sources in Nablus city in order to set up a sound policy on water management in Palestine. Methods This was a descriptive study carried out in two phases with a random sampling technique in the second phase. Primarily, samples were taken from 4 wells and 5 springs that supplied Nablus city residents. For each source, 3 samples were taken and each was analyzed in 4 cycles by RAD 7 device manufactured by Durridge Company. Secondly, from the seven regions of the Nablus city, three samples were taken from the residential tap water of each region. Regarding the old city, ten samples were taken. Finally, the mean radon concentration value for each source was calculated. Results The mean (range) concentration of radon in the main sources were 6.9 (1.5-23.4) Becquerel/liter (Bq/L). Separately, springs and wells' means were 4.6 Bq/L and 9.5 Bq/L; respectively. For the residential tap water in the 7 regions, the results of the mean (range) concentration values were found to be 1.0 (0.9-1.3) Bq/L. For the old city, the mean (range) concentration values were 2.3 (0.9-3.9) Bq/L. Conclusions Except for Al-Badan well, radon concentrations in the wells and springs were below the United State Environmental Protection Agency maximum contaminated level (U.S EPA MCL). The level was much lower for tap water. Although the concentration of radon in the tap water of old city were below the MCL, it was higher than other regions in the city. Preventive measures and population awareness on radon's exposure are recommended. PMID:22243625

  18. Geological Features Inferred from Local Seismic Tomography in the Sunda Strait and West Java regions, Indonesia

    NASA Astrophysics Data System (ADS)

    Nugraha, A. D.; Sakti, A. P.; Rohadi, S.; Widiyantoro, S.

    2012-12-01

    We have conducted seismic tomographic inversions to obtain a P-wave seismic velocity structure beneath the Sunda Strait and West Java regions, Indonesia. The Sunda Strait is located in a complex geological system i.e. in the transition from the oblique subduction beneath Sumatra to the nearly perpendicular subduction below Java. The Krakatau active volcano is located in the Sunda Strait. In this study, we have used selected P-wave arrival times from the data catalogs of the SeisComP-BMKG network (from 2009 to 2011) and the BMKG BALAI II network (from 1992 to 2011) compiled by Badan Meteorologi,Klimatologi dan Geofisika (BMKG), Indonesia. In total, there are 1,598 local earthquakes and 10,366 P-wave phases from 25 seismographic stations that have been used for the tomographic inversions. We have also relocated the hypocenter locations along with velocity inversions simultaneously. Our preliminary results depict some prominent geological features that include: (1) a low velocity anomaly beneath north of the Ujung Kulon region, which coincides with a low gravity anomaly resulting from a previous study, (2) a low velocity anomaly alignment beneath the Krakatau volcano in the Sunda Strait, (3) a sharp contrast in velocity anomalies extending from Pelabuhan Ratu towards Jakarta with a strike of SW-NE, and (4) a low velocity anomaly in the offshore of Pelabuhan Ratu that may be correlated with the continuation of the Cimandiri fault zone. More detailed information will be presented during the meeting. Keywords: tomography, Sunda Strait, West Java, velocity anomaly

  19. Field based geothermal exploration: Structural controls in the Tarutung Basin/North Central Sumatra (Indonesia)

    NASA Astrophysics Data System (ADS)

    Nukman, M.; Moeck, I.

    2012-04-01

    two million years due to the increase in sea-floor spreading rate of the Indian-Australian plate. The combination of regional clockwise rotation of Sumatra with local clockwise rotation caused by simple shear along the dextral SFS might generate the complex fault pattern which controls fluid flow of thermal water and placement of hot springs. Acknowledgements : Deutscher Akademischer Austausch Dienst, DAAD. German Ministry for Education and Research, BMBF. Badan Geologi - KESDM Bandung, Indonesia.

  20. Reflection seismic imaging in the volcanic area of the geothermal field Wayang Windu, Indonesia

    NASA Astrophysics Data System (ADS)

    Polom, Ulrich; Wiyono, Wiyono; Pramono, Bambang; Krawczyk, CharLotte M.

    2014-05-01

    Reflection seismic exploration in volcanic areas is still a scientific challenge and requires major efforts to develop imaging workflows capable of an economic utilization, e.g., for geothermal exploration. The SESaR (Seismic Exploration and Safety Risk study for decentral geothermal plants in Indonesia) project therefore tackles still not well resolved issues concerning wave propagation or energy absorption in areas covered by pyroclastic sediments using both active P-wave and S-wave seismics. Site-specific exploration procedures were tested in different tectonic and lithological regimes to compare imaging conditions. Based on the results of a small-scale, active seismic pre-site survey in the area of the Wayang Windu geothermal field in November 2012, an additional medium-scale active seismic experiment using P-waves was carried out in August 2013. The latter experiment was designed to investigate local changes of seismic subsurface response, to expand the knowledge about capabilities of the vibroseis method for seismic surveying in regions covered by pyroclastic material, and to achieve higher depth penetration. Thus, for the first time in the Wayang Windu geothermal area, a powerful, hydraulically driven seismic mini-vibrator device of 27 kN peak force (LIAG's mini-vibrator MHV2.7) was used as seismic source instead of the weaker hammer blow applied in former field surveys. Aiming at acquiring parameter test and production data southeast of the Wayang Windu geothermal power plant, a 48-channel GEODE recording instrument of the Badan Geologi was used in a high-resolution configuration, with receiver group intervals of 5 m and source intervals of 10 m. Thereby, the LIAG field crew, Star Energy, GFZ Potsdam, and ITB Bandung acquired a nearly 600 m long profile. In general, we observe the successful applicability of the vibroseis method for such a difficult seismic acquisition environment. Taking into account the local conditions at Wayang Windu, the method is

  1. The Ongoing Lava Flow Eruption of Sinabung Volcano (Sumatra, Indonesia): Observations from Structure-from-Motion and Satellite Remote Sensing

    NASA Astrophysics Data System (ADS)

    Carr, B. B.; Clarke, A. B.; Arrowsmith, R.; Vanderkluysen, L.

    2015-12-01

    . We use a pre-eruption DEM of Sinabung provided by the Badan Informasi Geospasial (Indonesia) to identify over 20 older lava flows at Sinabung. The active flow appears to represent a typical eruption of Sinabung, with its length and area similar to previous flows.

  2. A Framework for Monitoring and Maintenance of a Tsunami Early Warning System using ITIL®

    NASA Astrophysics Data System (ADS)

    Gensch, Stephan; Günther, Michael; Henneberger, Ralph; Strollo, Angelo

    2013-04-01

    Within this work, we present our approach and ongoing efforts to establish monitoring and maintenance processes for Tsunami Early Warning Systems. Practical work is done within the context of the Indonesian Tsunami Warning System (INATEWS) at Badan Meteorologi, Klimatologi dan Geofisika (BMKG) in Jakarta, Indonesia. The German contribution is well known as GITEWS. INATEWS is composed of several thousand integrated system components and numerous software processes. Due to the heterogeneity and complexity of the system, as well as the high availability needs, being an operational TEWS, real-time monitoring, reporting and scheduled preventive maintenance are needed. To develop and install an organizational and operational methodology for maintenance processes for INATEWS, we asserted ITIL® methods and are in development of Standard Operating Procedures (SOP) together with BMKG operational and management staff. ITIL®-conforming methods are one means of IT Service Management which has been adopted by a variety of service oriented IT providers. An early warning system does not expose classical consumer services, but the dissemination of warning messages and an early warning as a product may nevertheless be viewed as distinct services provided by a TEWS. We applied methods from ITIL® to the modular and hierarchical components of an early warning center, where minimum requirements on service availability, reliability and correctness of the warning product exist, from dissemination down to each sensor component. We describe functions of actors that ensure management of incidents and problems, as well as managing applications, IT operations and further technical issues. For the components of the early warning system, we present a model of event detection and event resolution. Real-time monitoring provides automated health-checks. Errors lead to reports to designated targets. Preventive maintenance provides findings on data and system availability, and data quality. Each

  3. Allelopathic Effects of Cyanobacterial Filtrates on Baltic Diatom

    NASA Astrophysics Data System (ADS)

    Śliwińska, Sylwia; Latała, Adam

    2012-01-01

    ównano wpływ przesączu komórkowego uzyskanego z hodowli sinic będących w fazie logarytmicznego i stacjonarnego wzrostu. Badania wykazały, że temperatura modyfikuje allelopatyczne oddziaływania i na przykład najwyższy spadek wzrostu zaobserwowano u S. marinoi po dodaniu przesączu uzyskanego z kultur N. spumigena hodowanych w 25°C. Wynosił on 70% w stosunku do kontroli. Ponadto w pracy stwierdzono, że N. spumigena wykazywała oddziaływanie allelopatyczne na badaną okrzemkę jedynie wtedy, gdy dodawany przesącz komórkowy pochodził z fazy logarytmicznego wzrostu. Wyniki uzyskane w niniejszej pracy sugerują, że bałtyckie sinice mogą wykazywać oddziaływania allelopatyczne w stosunku do okrzemek a produkcja związków allelopatycznych może być zależna od temperatury oraz fazy wzrostu, w której znajdują się organizmy donorowe.

  4. Sedimentary Rocks Associated with the Coal Seams of the Saddle Beds from the Chwałowice Trough - West Part of Upper Silesian Coal Basin / Skały Osadowe Towarzyszące Pokładom Węgla Warstw Siodłowych Niecki Chwałowickiej - Zachodnia Część Górnośląskiego Zagłębia Węglowego

    NASA Astrophysics Data System (ADS)

    Stanienda, Katarzyna

    2015-03-01

    łów ilastych, takich jak kaolinit i illit, węglanów i chalcedonu, chociaż wyniki dyfraktometrii rentgenowskiej potwierdziły występowanie w spoiwie badanych piaskowców również halitu. Na podstawie składu mineralnego piaskowców, dokonano ich klasyfikacji, stosując podziały skał okruchowych Krynina (1948) i Pettijohn'a-Potter'a-Siever'a (1973). Według klasyfikacji Krynina badane piaskowce reprezentują szarogłazy (szarowaki). W klasyfikacji Pettijohn'a, próbki 6, 8 i 13 reprezentują arenity lityczne, natomiast próbki 3 i 10- waki. Mułowce i iłowce zbudowane SA głównie z minerałów ilastych. Można w nich również spotkać ziarna kwarcu oraz miki. Wyższy udział kwarcu i mik jest jednak charakterystyczny dla mułowców. Skały towarzyszące pokładom węgla warstw siodłowych Niecki Chwałowickiej zawierają również domieszki substancji organicznej.

  5. The Change of Structural and Thermal Properties of Rocks Exposed to High Temperatures in the Vicinity of Designed Geo-Reactor / Zmiany właściwości strukturalnych i cieplnych skał poddanych wysokim temperaturom w rejonie projektowanego georeaktora

    NASA Astrophysics Data System (ADS)

    Małkowski, Piotr; Niedbalski, Zbigniew; Hydzik-Wiśniewska, Joanna

    2013-06-01

    ści na skutek wysokiej temperatury oraz skonfrontowano powyższe wyniki z wynikami badań przewodności cieplnej, pojemności cieplnej i dyfuzyjności cieplnej (przewodzenia temperatury) skał. Na podstawie opisu mineralogicznego skał przed wygrzaniem stwierdzono, że są to okruchowe skały osadowe z frakcją psamityczną. Szkielet ziarnowy jest zbudowany niemal wyłącznie z ziaren kwarcu, a podrzędnie w skałach występują muskowit, biotyt, skalenie i minerały ciężkie. Zawartość procentowa minerałów i spoiwa zmienia się w szerokim zakresie: ziaren mineralnych od 10,3% do 90,0%, a spoiwa od 10,0% do 89,7%. Po przepaleniu skał do temperatury 1000÷1200°C zmniejsza się w nich zawartość matrix, a wzrasta zawartość kwarcu. Pojawiają się także nowe minerały, takie jak hematyt i minerały rudne. Po ogrzaniu nie stwierdzono cementu węglanowego, opali, tlenowodorków żelaza, kalcytu, hornblendy i porów. Proporcja pomiędzy ziarnami a spoiwem ulega zupełnej zmianie i ziarna stanowią 31-99% objętości, a spoiwo - 1-69%. Wpływ wysokiej temperatury na zmianę struktury i tekstury skał określano obserwując zachowanie się skał w trakcie ich wygrzewania. W pierwszej kolejności zaobserwowano zdecydowanie różny charakter reagowania poszczególnych typów skał na wysoką temperaturę, a w swoich fazach podobny do obserwacji poczynionych przez Mao (Mao et al. 2008). Łupki ilaste wypalały się całkowicie, zmieniały swój kolor na brązowy, a także rozwarstwiały i pękały na drobne kawałki. Łupki piaszczyste generalnie nie zmieniały swojej postaci, lecz często pękały wzdłuż powierzchni uwarstwienia. Laminy materiałów ilastych ulegały przepaleniu i kolor zmieniał się na brązowy lub brunatno-czerwony. Piaskowce całkowicie zachowywały swój kształt, natomiast niektóre minerały zmieniały swój kolor na czerwony lub brązowy. Wykonane badania pokazują, że wszystkie badane skały po wyprażeniu zwiększają swoją gęstość obj

  6. Sorption of Cd2+ Ions From Aqueous Solutions on Organic Wastes / Sorpcja Jonów Cd2+ Z Roztworów Wodnych Na Odpadach Organicznych

    NASA Astrophysics Data System (ADS)

    Bożęcka, Agnieszka; Sanak-Rydlewska, Stanisława

    2015-09-01

    This article presents the results of research on the Cd2+ ions sorption from model aqueous solutions on sunflower hulls, walnut shells and plum stones. The effect of various factors, such as mass of the natural sorbent, the pH, the time and the temperature was studied. The process of Cd2+ ions sorption on studied sorbents was described by the Langmuir model. The best sorption capacity has been achieved for sunflower hulls. The maximum sorption capacity for this material was 19.93 mg/g. W artykule przedstawiono wyniki badań, które dotyczyły usuwania jonów Cd2+ z modelowych roztworów wodnych za pomocą odpadów organicznych, takich jak: łuski słonecznika, łupiny orzecha włoskiego i pestki śliwek. Wykazano, iż badane materiały mogą być skutecznie wykorzystywane do usuwania jonów Cd2+ z modelowych roztworów wodnych w układach jednoskładnikowych. Dla badanego zakresu stężeń i przyjętych warunków procesu sorpcji w układach jednoskładnikowych, największą wydajność sorpcji jonów Cd2+, osiągnięto dla łuszczyn słonecznika. Wyniosła ona 81,75-93,02%. Dla pozostałych materiałów sorpcja jest nieco niższa, ale również zadowalająca. W pracy podano interpretację otrzymanych wyników w oparciu o jeden z najpopularniejszych modeli izoterm adsorpcji - Langmuira, który potwierdził, iż najlepszym sorbentem jonów Cd2+, spośród badanych, są łuszczyny słonecznika. Materiał ten cechuje się największą wartością parametrów qmax i b izotermy Langmuira. W tym przypadku stała qmax, wyrażająca pojemność monowarstwy, przyjęła wartość 19,93 mg/g, a parametr b, określający powinowactwo do usuwanych jonów wynosi 0,2264 dm3/mg (Rys. 5, Tab. 1). Udowodniono również, że proces sorpcji jonów Cd2+ na badanych sorbentach organicznych zależy od masy sorbentu. Dla wszystkich materiałów stopień usunięcia jonów Cd2+ z roztworów wodnych rośnie ze wzrostem masy sorbentu, aż do uzyskania maksimum przy naważce 0,5 g (Rys. 1

  7. Free Surface of the Liquid-Gas Phase Separation as a Measuring Membrane of a Device for Measuring Small Hydrostatic Pressure Difference Values / Powierzchnia Swobodna Rozdziału Faz Ciecz-Gaz Jako Membrana Pomiarowa Urządzenia Do Pomiaru Małych Wartości Różnicy Ciśnienia Hydrostatycznego

    NASA Astrophysics Data System (ADS)

    Filipek, Wiktor; Broda, Krzysztof; Branny, Marian

    2015-03-01

    wykorzystaniem napisanego w języku Delphi autorskiego programu do obróbki numerycznej zdjęć. Występujące trudności ilustruje rysunek 2 przedstawiający przesunięcie linii odniesienia w trzech kolejno po sobie wykonanych zdjęciach. Następnie omówiono sposób przygotowania stanowiska do pomiarów oraz sposób ich przeprowadzania czego ilustracją są rysunki 3 i 4. Przedstawiono występujące problemy oraz zastosowane sposoby ich rozwiązania. Zarejestrowany przykładowy obraz po przejściu promienia lasera przez badaną strukturę dla wybranych wartości różnicy ciśnień hydrostatycznych uzyskany za pomocą kulek ceramicznych przedstawiono na rysunku 5. Rysunek 6 wyjaśnia metodę obróbki numerycznej zdjęć w oparciu o autorski program i zależności (1),(2),(3). Na rysunkach 7 i 9 zestawiono otrzymane krzywe zmienności intensywności barw uzyskane w trakcie opracowania numerycznego. Zwrócono także uwagę na rozkład zmian intensywności barw dla różnicy między powierzchniami swobodnymi cieczy w cylindrach (Rys. 8-11) dla wybranych serii pomiarowych. W dalszej części artykułu dokonano analizy otrzymanych obrazów zwracając uwagę na włączenie metody transformacji Fouriera lub "Falkowej" (Ziółko, 2000) do numerycznej analizy posiadanych danych (Rys. 12-14). Na rysunku 12 pokazano wybrane trzy zależności opisujące zmienność natężania barwy w funkcji położenia ramki (1), dla których dokonano transformacji Fouriera według zależności (3). Analizy dokonano metodą arytmetyczna Perry'ego (Ziółko, 2000) w okienku o szerokości n = 2300 przyjmując jako położenie startowe wartość x = 1600. Rysunek 13 przedstawia wartości współczynnika Ak a rys.14 przedstawia wartości kąta przesunięcia jk dla pierwszych 58 harmonicznych. Prace nad udoskonaleniem metody trwają. W dalszej części artykułu autorzy podsumowują osiągnięte wyniki zwracając uwagę na uzyskaną precyzję pomiarów oraz korzystne zastosowanie powierzchni swobodnej

  8. Visualization of Multidimensional Data in Purpose of Qualitative Classification of Various Types of Coal / Wizualizacja Wielowymiarowych Danych W Celu Klasyfikacji JAKOŚCIOWEJ RÓŻNYCH TYPÓW WĘGLA

    NASA Astrophysics Data System (ADS)

    Niedoba, Tomasz; Jamróz, Dariusz

    2013-12-01

    węgla łącznie. Zdecydowano się na zastosowanie nowatorskich metod wizualizacji wielowymiarowych danych, którymi były metoda tuneli obserwacyjnych oraz metoda osi równoległych. Zasady i metodyka badań zostały przedstawione w podrozdziałach 2 i 3. Zastosowane metody umożliwiły uzyskanie wizualizacji siedmiowymiarowych danych opisujących węgiel. Za pomocą tych wizualizacji możliwe jest zaobserwowanie wyraźnego podziału przestrzeni cech pomiędzy badanymi typami węgla. Metody te umożliwiły spojrzenie na badane dane z różnych perspektyw, które pozwalają na stwierdzenie zasadniczych różnic badanych materiałów. Dla badanych węgli stwierdzono wyraźne takie różnice co świadczy o tym, że za pomocą proponowanych metod możliwa jest skuteczna identyfikacja typu węgla, jak również dokładniejsza analiza jego poszczególnych cech i identyfikacja np. klasy ziarnowej. Szczegółowe obrazy i ich interpretacja zostały przedstawione w rozdziale 3 i we wnioskach końcowych. Rysunki 3-5 obrazują różnice pomiędzy poszczególnymi typami węgla otrzymane metodą tuneli obserwacyjnych. Wyraźnie można rozgraniczyć próbki dotyczące poszczególnych węgli a tym samym możliwa jest identyfikacja typu węgla na podstawie wielowymiarowej analizy. Rysunki 6-7 pokazują zastosowanie innej metody wielowymiarowej, którą była metoda osi równoległych. Metoda ta okazała się być skuteczna do uzyskania informacji o konieczności przeskalowania poszczególnych cech, w celu uzyskania bardziejczytelnych rezultatów. Natomiast rysunek 10 pokazuje różnice otrzymane metodą tuneli obserwacyjnych pomiędzy charakterystykami konkretnych klas ziarnowych wybranego materiału, którym w tym przypadku był węgiel typu 31. Uzyskane wyniki i zastosowana metodyka są nowatorskie i stanowią bazę pod bardziej szczegółowe badania, biorące pod uwagę także inne charakterystyki węgli, w tym ich strukturę i teksturę. Za pomocą przedstawionych metod mo

  9. Influence of the Plow Filling and Thread Angle onto the Plow Head Efficiency / Wpływ Współczynnika Wypełnienia Organu Oraz Kąta Nawinięcia Płata Ślimaka Na Sprawność Ładowania Frezującymi Organami Ślimakowymi

    NASA Astrophysics Data System (ADS)

    Wydro, Tomasz

    2015-03-01

    silniku posuwu w funkcji sprawność ładowania ηl. Uzyskane wyniki i ich analiza pozwoliły stwierdzić, że badane organy wykazywały bardzo zróżnicowane sprawności ładowania, w zależności od zmiennych parametrów ruchowych, czyli prędkości posuwu vp i obrotów n, a także przy różnym kącie nawinięcia płata ślimaka. Również istotnym wnioskiem z badań, który może w praktyce zostać wykorzystany, jest fakt, że dla stałych obrotów organu n i zwiększanej prędkości posuwu vp spadała sprawność ładowania ηł (dla pracy organu bez ładowarki). Fakt ten jest ważny, ponieważ w warunkach dołowych w trakcie eksploatacji kombajnu ścianowego istnieje tylko możliwość regulacji jego prędkości posuwu. Istotnym dla wykorzystania w praktyce, może być również fakt, iż przy pracy organu z ładowarką występuje wzrost poboru mocy organu przy wzroście współczynnika wypełnienia kw. Jest to istotne z punktu widzenia ekonomi eksploatacji i dążenia do minimalnego zużycia energii. Zrealizowane badania laboratoryjne sprawności procesu ładowania, pozwoliły uzyskać szeroki zakres wyników, które mogą pomóc przy doborze parametrów ruchowych kombajnu ścianowego w trakcie jego eksploatacji, a nawet wcześniej, na etapie projektowania organów ślimakowych (Wydro, 2011).