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Sample records for pueraria montana community

  1. Kudzu (Pueraria montana) community responses to herbicides, burning, and high-density loblolly pine.

    Treesearch

    Timothy B. Harrington; Laura T. Rader-Dixon; John W. Jr. Taylor

    2003-01-01

    Kudzu is an aggressive, nonnative vine that currently dominates an estimated 810,000 ha of mesic forest communities in the eastern United States. To test an integrated method of weed control, abundances of kudzu and other plant species were compared during 4 yr after six herbicide treatments (clopyralid, triclopyr, metsulfuron, picloram 1 2,4-D, tebuthiuron, and a...

  2. Kudzu (Pueraria montana) community responses to herbicides, burning, and high-density loblolly pine

    SciTech Connect

    T.B. Harrington; L.T. Rader-Dixon; J.W. Taylor, Jr.

    2003-11-01

    Kudzu is an aggressive, nonnative vine that currently dominates an estimated 810,000 ha of mesic forest communities in the eastern United States. To test an integrated method of weed control, abundances of kudzu and other plant species were compared during 4 yr after six herbicide treatments (clopyralid, triclopyr, metsulfuron, picloram 1 2,4-D, tebuthiuron, and a nonsprayed check), in which loblolly pines were planted at three densities (0, 1, and 4 seedlings m22) to induce competition and potentially delay kudzu recovery. This split-plot design was replicated on each of the four kudzu-dominated sites near Aiken, SC. Relative light intensity (RLI) and soil water content (SWC) were measured periodically to identify mechanisms of interference among plant species. Two years after treatment (1999), crown coverage of kudzu averaged , 2% in herbicide plots compared with 93% in the nonsprayed check, and these differences were maintained through 2001, except in clopyralid plots where kudzu cover increased to 15%. In 2001, pine interference was associated with 33, 56, and 67% reductions in biomass of kudzu, blackberry, and herbaceous vegetation, respectively. RLI in kudzu-dominated plots (4 to 15% of full sun) generally was less than half that of herbicide-treated plots. SWC was greatest in tebuthiuron plots, where total vegetation cover averaged 26% compared with 77 to 111% in other plots. None of the treatments eradicated kudzu, but combinations of herbicides and induced pine competition delayed its recovery.

  3. Ovularia puerariae Sawada is the hyphomycetous anamorph of a new Marasmius species on living leaves of kudzu (Pueraria montana, Fabaceae).

    PubMed

    Kirschner, Roland; Lee, I-Shu; Chen, Chee-Jen

    2013-01-01

    An arthroconidial hyphomycete on living leaves of kudzu (Pueraria montana, Fabaceae), originally described by Sawada in 1959 as Ovularia puerariae, was rediscovered. This anamorph is connected to an unknown Marasmius teleomorph belonging to section Globulares, which develops on the same living leaves. Ultrastructure and LSU rDNA sequence analysis of the anamorph confirm this connection. The fungus does not have only a unique biology among agarics, comparable only to Mycena citricolor, but also has the potential for application as a control agent of kudzu. During comparison with similar anamorph genera, Illosporium graminicola was found to be a synonym for Beniowskia sphaeroidea.

  4. Kudzu [Pueraria montana (Lour.) Merr. Var lobata]: a new source of carbohydrate for bioethanol production

    USDA-ARS?s Scientific Manuscript database

    We determined the amount of standing biomass of kudzu (Pueraria montana var. lobata) in naturally-infested fields in Maryland and Alabama, USA. At each site, we evaluated carbohydrate content of root, stems and leaves. For a third site from Georgia, we evaluated the carbohydrate content of roots o...

  5. First Report of Soybean Rust, Caused by Phakopsora pachyrhizi, on Kudzu (Pueraria montana var. lobata) in Illinois

    USDA-ARS?s Scientific Manuscript database

    Soybean rust, caused by Phakopsora pachyrhizi, was first observed on soybean (Glycine max) in the continental United States in Louisiana in 2004, and on kudzu (Pueraria montana var. lobata) in the United States in 2005. Kudzu is a leguminous weed that is prevalent in the southern United States with ...

  6. Kudzu (Pueraria montana) Invasion Doubles Emissions of Nitric Oxide, a Precursor to Tropospheric Ozone.

    NASA Astrophysics Data System (ADS)

    Hickman, J. E.; Wu, S.; Mickley, L. J.; Lerdau, M. T.

    2008-12-01

    Nitrogen-fixing plants can increase rates of nitrogen (N) cycling in soils, fluxes of the greenhouse gas nitrous oxide (N2O), and fluxes of the ozone precursor nitric oxide (NO). Invasion by the nitrogen-fixing legume kudzu (Pueraria montana) across millions of hectares in the southeastern United States could be contributing to increased ozone concentrations. Ozone formation in the Southeast is broadly limited by atmospheric NO concentrations, so perturbations to the N cycle by kudzu have the potential to increase ozone concentrations in the region. At three sites in Madison County, Georgia, kudzu invasion increased rates of net N mineralization by up to an order of magnitude and rates of net nitrification and soil pools of NO3- and NO2- by up to 500%. Emissions of NO from soils invaded by kudzu averaged 2.81 ng NO-N cm-2 h-1, significantly higher than emissions from soil dominated by native vegetation, which averaged 1.24 ng NO-N cm-2 h-1. Emissions of N2O display a trend towards increasing under kudzu invasion, but N2O emissions at these sites were three orders of magnitude smaller than emissions of NO. Atmospheric chemical modeling suggests that these higher NO emissions could increase ozone concentrations in the region by up to 2 ppb. We propose that kudzu invasion in the southeastern United States represents a novel threat to air quality and could increase the frequency with which federal ozone standards are exceeded.

  7. Heterogeneity of clonal patterns among patches of kudzu, Pueraria montana var. lobata, an invasive plant.

    PubMed

    Kartzinel, Tyler R; Hamrick, J L; Wang, Chongyun; Bowsher, Alan W; Quigley, Bryan G P

    2015-10-01

    Viny species are among the most serious invasive plants, and better knowledge of how vines grow to dominate landscapes is needed. Patches may contain a single genotype (i.e. genet), a competitively dominant genet or many independent but interacting genets, yet the clonal structure of vining species is often not apparent. Molecular markers can discriminate among the genetic identities of entwined vines to reveal the number and spatial distribution of genets. This study investigated how genets are spatially distributed within and among discrete patches of the invasive vine kudzu, Pueraria montana var. lobata, in the United States. It was expected that ramets of genets would be spatially clustered within patches, and that an increase in the number of genets within a patch would be associated with a decrease in the average size of each genet. Six discrete kudzu patches were sampled across 2 years, and 1257 samples were genotyped at 21 polymorphic allozyme loci. Variation in genotypic and genetic diversity among patches was quantified and patterns of genet interdigitation were analysed. Substantial genotypic and genetic variation occurred within and among patches. As few as ten overlapping genets spanned up to 68 m(2) in one patch, while >90 % of samples were genetically unique in another patch. Genotypic diversity within patches increased as mean clone size decreased, although spatially widespread genets did not preclude interdigitation. Eight genets were shared across ≥2 patches, suggesting that vegetative dispersal can occur among patches. Genetically unique kudzu vines are highly interdigitated. Multiple vegetative propagules have become established in spatially discrete patches, probably through the movement of highway construction or maintenance machinery. The results suggest that common methods for controlling invasive vines (e.g. mowing) may inadvertently increase genotypic diversity. Thus, understanding vine architecture and growth has practical

  8. Kudzu (Pueraria montana) invasion doubles emissions of nitric oxide and increases ozone pollution

    PubMed Central

    Hickman, Jonathan E.; Mickley, Loretta J.; Lerdau, Manuel T.

    2010-01-01

    The nitrogen-fixing legume kudzu (Pueraria montana) is a widespread invasive plant in the southeastern United States with physiological traits that may lead to important impacts on ecosystems and the atmosphere. Its spread has the potential to raise ozone levels in the region by increasing nitric oxide (NO) emissions from soils as a consequence of increasing nitrogen (N) inputs and cycling in soils. We studied the effects of kudzu invasions on soils and trace N gas emissions at three sites in Madison County, Georgia in 2007 and used the results to model the effects of kudzu invasion on regional air quality. We found that rates of net N mineralization increased by up to 1,000%, and net nitrification increased by up to 500% in invaded soils in Georgia. Nitric oxide emissions from invaded soils were more than 100% higher (2.81 vs. 1.24 ng NO-N cm−2 h−1). We used the GEOS-Chem chemical transport model to evaluate the potential impact of kudzu invasion on regional atmospheric chemistry and air quality. In an extreme scenario, extensive kudzu invasion leads directly to an increase in the number of high ozone events (above 70 ppb) of up to 7 days each summer in some areas, up from 10 to 20 days in a control scenario with no kudzu invasion. These results establish a quantitative link between a biological invasion and ozone formation and suggest that in this extreme scenario, kudzu invasion can overcome some of the air quality benefits of legislative control. PMID:20479252

  9. Heterogeneity of clonal patterns among patches of kudzu, Pueraria montana var. lobata, an invasive plant

    PubMed Central

    Kartzinel, Tyler R.; Hamrick, J. L.; Wang, Chongyun; Bowsher, Alan W.; Quigley, Bryan G. P.

    2015-01-01

    Background and Aims Viny species are among the most serious invasive plants, and better knowledge of how vines grow to dominate landscapes is needed. Patches may contain a single genotype (i.e. genet), a competitively dominant genet or many independent but interacting genets, yet the clonal structure of vining species is often not apparent. Molecular markers can discriminate among the genetic identities of entwined vines to reveal the number and spatial distribution of genets. This study investigated how genets are spatially distributed within and among discrete patches of the invasive vine kudzu, Pueraria montana var. lobata, in the United States. It was expected that ramets of genets would be spatially clustered within patches, and that an increase in the number of genets within a patch would be associated with a decrease in the average size of each genet. Methods Six discrete kudzu patches were sampled across 2 years, and 1257 samples were genotyped at 21 polymorphic allozyme loci. Variation in genotypic and genetic diversity among patches was quantified and patterns of genet interdigitation were analysed. Key Results Substantial genotypic and genetic variation occurred within and among patches. As few as ten overlapping genets spanned up to 68 m2 in one patch, while >90 % of samples were genetically unique in another patch. Genotypic diversity within patches increased as mean clone size decreased, although spatially widespread genets did not preclude interdigitation. Eight genets were shared across ≥2 patches, suggesting that vegetative dispersal can occur among patches. Conclusions Genetically unique kudzu vines are highly interdigitated. Multiple vegetative propagules have become established in spatially discrete patches, probably through the movement of highway construction or maintenance machinery. The results suggest that common methods for controlling invasive vines (e.g. mowing) may inadvertently increase genotypic diversity. Thus, understanding

  10. Kudzu (Pueraria montana) invasion doubles emissions of nitric oxide and increases ozone pollution.

    PubMed

    Hickman, Jonathan E; Wu, Shiliang; Mickley, Loretta J; Lerdau, Manuel T

    2010-06-01

    The nitrogen-fixing legume kudzu (Pueraria montana) is a widespread invasive plant in the southeastern United States with physiological traits that may lead to important impacts on ecosystems and the atmosphere. Its spread has the potential to raise ozone levels in the region by increasing nitric oxide (NO) emissions from soils as a consequence of increasing nitrogen (N) inputs and cycling in soils. We studied the effects of kudzu invasions on soils and trace N gas emissions at three sites in Madison County, Georgia in 2007 and used the results to model the effects of kudzu invasion on regional air quality. We found that rates of net N mineralization increased by up to 1,000%, and net nitrification increased by up to 500% in invaded soils in Georgia. Nitric oxide emissions from invaded soils were more than 100% higher (2.81 vs. 1.24 ng NO-N cm(-2) h(-1)). We used the GEOS-Chem chemical transport model to evaluate the potential impact of kudzu invasion on regional atmospheric chemistry and air quality. In an extreme scenario, extensive kudzu invasion leads directly to an increase in the number of high ozone events (above 70 ppb) of up to 7 days each summer in some areas, up from 10 to 20 days in a control scenario with no kudzu invasion. These results establish a quantitative link between a biological invasion and ozone formation and suggest that in this extreme scenario, kudzu invasion can overcome some of the air quality benefits of legislative control.

  11. Regulation of isoflavone production in hydroponically grown Pueraria montana (kudzu) by cork pieces, XAD-4, and methyl jasmonate.

    PubMed

    Kirakosyan, Ara; Kaufman, Peter B; Chang, Soo Chul; Warber, Sara; Bolling, Steven; Vardapetyan, Hrachik

    2006-12-01

    A mini-hydroponic growing system was employed for seedlings of kudzu vine (Pueraria montana) and contents of isoflavones (daidzein, genistein, daidzin, genistin, and puerarin) from shoot and root parts of seedlings were analyzed quantitatively. In addition, exogenous cork pieces, polymeric adsorbent, XAD-4, and universal elicitor, methyl jasmonate (MeJA), were used to regulate the production of these isoflavones. It was shown that cork pieces up-regulate the production of daidzein and genistein up to seven- and eight-fold greater than the levels obtained for control roots. In contrast, levels of glucosyl conjugates, daidzin and genistin, decrease up to five- and eight-fold, respectively. Cork treatment also induces the excretion of the root isoflavone constituents into the growth medium. Minimal levels of isoflavones are absorbed by the cork pieces. XAD-4 stimulates the production of glucosyl conjugates, daidzin and genistin, in root parts about 1.5-fold greater than that obtained in control roots. These are the highest amounts of daidzin and genistin that are observed (5.101 and 6.759 mg g(-1) dry weight, respectively). In contrast to these two adsorbents, MeJA increases the accumulation of isoflavones in shoot rather than in root parts of seedlings, about three- to four-fold over control levels, with the exception of genistein. These studies reveal new observations on the regulation of isoflavone production in hydroponically grown Pueraria montana plants by two adsorbents (cork pieces and XAD-4) and MeJA elicitor.

  12. An overexpression of chalcone reductase of Pueraria montana var. lobata alters biosynthesis of anthocyanin and 5'-deoxyflavonoids in transgenic tobacco.

    PubMed

    Joung, Jae-youl; Kasthuri, G Mangai; Park, Ji-young; Kang, Won-jin; Kim, Hyun-soon; Yoon, Bong-sik; Joung, Hyouk; Jeon, Jae-heung

    2003-03-28

    We isolated the chalcone reductase (pl-chr) gene of Pueraria montana var. lobata by using a PCR strategy from cDNA pools of storage roots. A high level of expression of RNA was found in both stems and roots. The genomic Southern blot result suggests that pl-chr exists as a member of a small gene family. By introducing a pl-chr gene under the control of the 35S CaMV promoter into the pink-flowering Xanthi line of Nicotiana tabacum, the flower color was changed from pink to white-to-pink. The contents of anthocyanin in the flowers of the transgenic lines were dramatically decreased by 40%, but the total UV absorption compounds remained unchanged. The production of liquiritigenin in pl-chr overexpressed transgenic tobacco lines was confirmed by HPLC and MS analysis. The introduction of pl-chr gene provides a method to redirect the flavonoid pathway into 5'-deoxyflavonoid production in non-legume crops, in order to manipulate the phenylpropanoid pathway for isoflavonoid production.

  13. Community Education in Montana. The Broadview Model.

    ERIC Educational Resources Information Center

    Lewis, Ray

    Four counties and five adjacent towns are served by the Broadview, Montana (population 100), community education program begun in 1978. A one mill tax levy and a 3-year Mott fund seed grant allowed for tuition courses in 1979-80; in 1981, a $5 per course fee will be charged. The majority of courses are taught in the school and are designed to…

  14. Leaching and persistence of herbicides for kudzu (Pueraria montana) control on pine regeneration sites

    SciTech Connect

    Berisford, Yvette, C.; Bush, Parshall, B.; Taylor, John, W.

    2006-03-01

    Kudzu is an exotic vine that threatens forests in the southeastern United States. It can climb, overtop, and subsequently kill new seedlings or mature trees. Herbicides are commonly used to control kudzu; however, eradication might require retreatment for 3 to 10 yr in young stands and 7 to 10 yr for mature stands. Clopyralid, picloram, triclopyr, metsulfuron, and tebuthiuron exert various degrees of control, depending on soil type, meteorological conditions, herbicide formulation, seasonal application, characteristics of the kudzu stand, and frequency and number of herbicide. Field residue data for soil or leachate are lacking for all of these herbicides when they are used in actual forest regeneration programs in the Coastal Plain. These data are needed to assess the relative potential for the herbicides to leach into groundwater or to move off-site into sensitive ecological areas of the Coastal Plain in which sandy soils predominate and the groundwater tends to be shallow. As part of an integrated pest management program to control kudzu on forest regeneration areas at the Savannah River Site near New Ellenton, SC, five herbicides were evaluated from the standpoints of herbicide leaching, kudzu control, and plant community development. Three herbicide chemical families were represented. This included pyridinecarboxylic acid herbicides (clopyralid, picloram 1 2,4-D, and triclopyr), a sulfonylurea herbicide (metsulfuron), and a substituted urea herbicide (tebuthiuron).

  15. Kudzu Pueraria montana

    Treesearch

    James H. Miller

    1999-01-01

    Plant: Twining and trailing semi-woody vine, 10-30 m (35-100 ft) long, with rope-like vines covering mature trees and forming dense patches, having 3-leaflet leaves on hairy pctioles and stems, deciduous and dying with first frost, yielding fragrant red-purple flowers in mid- summer. and hairy flat capsules with few seeds in fall. Large, semi-...

  16. Plant community variability on a small area in southeastern Montana

    Treesearch

    James G. MacCracken; Daniel W. Uresk; Richard M. Hansen

    1984-01-01

    Plant communities are inherently variable due to a number of environmental and biological forces. Canopy cover and aboveground biomass were determined for understory vegetation in plant communities of a prairie grassland-forest ecotone in southeastern Montana. Vegetation units were described using polar ordination and stepwise discriminant analysis. Nine of a total of...

  17. High degree of clonal reproduction and lack of large-scale geographic patterning mark the introduced range of the invasive vine, kudzu (Pueraria montana var. lobata), in North America.

    PubMed

    Bentley, Kerin E; Mauricio, Rodney

    2016-08-01

    Pueraria montana var. lobata, or kudzu, is an invasive species whose invasion in North America is not genetically well characterized. The clonality of kudzu introduces challenges to population genetic analyses that can bias the assessment of spatial patterns of genotypes. Assessing patterns of genetic diversity while considering clonality is necessary to understand the invasion and spread of kudzu in its invasive range. We screened 1747 individuals from 87 populations across the invasive range with 15 microsatellite markers and a 789 bp chloroplast region. We performed detailed clonal analyses and tested levels of genetic diversity, population structure, and phylogeographic relationships. Kudzu exhibited a clonal rate of 80%, and was more heterozygous than other long-lived perennials. We detected only 353 distinct clonal lineages, with over 60% sharing a maternal haplotype. Populations were established with few genotypes, many consisting of only a single clone. We found no isolation by distance. Despite high genetic diversity, we found little geographic patterning. Kudzu is highly clonal with few genetically distinct lineages and haplotypes existing in the introduced range. Our data are consistent with a large single introduction, or a few at most. Introduced lineages are geographically randomly distributed but isolated, suggesting that genotypes rarely expand into already established populations. No route of expansion was detectable from an original introduction. The invasion of kudzu does not seem to have been dominated by a single genotype, thus standing genetic variation and phenotypic plasticity are more likely mechanisms explaining kudzu's invasion success. © 2016 Botanical Society of America.

  18. "Just Following the Buffalo": Origins of a Montana Metis Community

    ERIC Educational Resources Information Center

    Foster, Martha Harroun

    2006-01-01

    By 1879 the vast buffalo herds were all but gone from the Great Plains. Many of the remaining animals had moved south from the Milk River of northern Montana and Alberta into the Judith Basin of central Montana. In these rich grasslands, for a few more years, life went on as it had for centuries. Following the buffalo came many Indian bands, as…

  19. "Just Following the Buffalo": Origins of a Montana Metis Community

    ERIC Educational Resources Information Center

    Foster, Martha Harroun

    2006-01-01

    By 1879 the vast buffalo herds were all but gone from the Great Plains. Many of the remaining animals had moved south from the Milk River of northern Montana and Alberta into the Judith Basin of central Montana. In these rich grasslands, for a few more years, life went on as it had for centuries. Following the buffalo came many Indian bands, as…

  20. Community Strategic Visioning as a Method to Define and Address Poverty: An Analysis from Select Rural Montana Communities

    ERIC Educational Resources Information Center

    Lachapelle, Paul; Austin, Eric; Clark, Daniel

    2010-01-01

    Community strategic visioning is a citizen-based planning process in which diverse sectors of a community collectively determine a future state and coordinate a plan of action. Twenty-one communities in rural Montana participated in a multi-phase poverty reduction program that culminated in a community strategic vision process. Research on this…

  1. Community Strategic Visioning as a Method to Define and Address Poverty: An Analysis from Select Rural Montana Communities

    ERIC Educational Resources Information Center

    Lachapelle, Paul; Austin, Eric; Clark, Daniel

    2010-01-01

    Community strategic visioning is a citizen-based planning process in which diverse sectors of a community collectively determine a future state and coordinate a plan of action. Twenty-one communities in rural Montana participated in a multi-phase poverty reduction program that culminated in a community strategic vision process. Research on this…

  2. Relationship between Participation in 4-H and Community Leadership in Rural Montana

    ERIC Educational Resources Information Center

    Flynn, Allison; Frick, Martin; Steele, Douglas

    2010-01-01

    Studies on the impact of 4-H on former members generally use alumni as one cohort. In rural states, such as Montana, it is important to understand the impact of 4-H on alumni in these rural areas and the role 4-H plays in community involvement. The study reported here sought to determine the perception of current community leaders in rural Montana…

  3. Survey of phytophagous insects and foliar pathogens in China for a biocontrol perspective on kudzu, Pueraria montana var. lobata (Willd.) Maesen and S. Almeida (Fabaceae)

    Treesearch

    Jiang-Hua Sun; Zhu-Dong Liu; Kerry O. Britton; Ping Cai; David Orr; Judith Hough-Goldstein

    2006-01-01

    A three-year survey of kudzu foliage, seed, stems, and roots for associated phytophagous insects was conducted to establish basic information about the insect communities that kudzu harbors in China and to assess the abundance, diversity and damage caused by these insects. Diseases of kudzu were also surveyed in southern China. A total of 116 phytophagous insect...

  4. Enrollment in Community Colleges in the State of Montana, Fall 1985.

    ERIC Educational Resources Information Center

    Montana State Office of the Commissioner of Higher Education, Helena.

    A summary is provided of Montana community college enrollment data for fall 1985, with comparative statistics offered for previous years. Data are provided with respect to total enrollment; distribution of enrollment by institution, by class, and by sex; transfer students; student geographic origins; first-time students; full- versus part-time…

  5. Fruits and foliage of Pueraria (Leguminosae, Papilionoideae) from the Neogene of Eurasia and their biogeographic implications.

    PubMed

    Wang, Qi; Manchester, Steven R; Dilcher, David L

    2010-12-01

    Pueraria (Leguminosae, Papilionoideae) is native in East Asia, South Asia, Southeast Asia, and Oceania and is well known as a rampant invasive weed in the southeastern United States (P. montana; better known as kudzu), but relatively little is known about its early evolution and biogeographic origin. • On the basis of comparative analyses of the fruit and leaflet architecture of closely related extant and fossil taxa, we studied the fossil history and biogeography of Pueraria. • Fossil Pueraria is recognized on the basis of distinctive fruit and foliage from the Mio-Pliocene of middle latitudes in China, Japan, Abkhazia, and Croatia. Recognition of P. miothunbergiana from the Mio-Pliocene of China and Japan is reinforced by a trifoliolate leaf as well as isolated lateral and terminal leaflets. Pueraria shanwangensis sp. nov. represents the first recognition of fossil Pueraria fruits. This fruit species co-occurs with P. miothunbergiana in the Middle Miocene Shanwang flora and possibly represents the same population. Pueraria maxima (Unger) comb. nov., previously named as Dolichites maximus or Desmodium maximum, is recognized on the basis of leaflets from the Miocene of Croatia and Abkhazia. Other prior fossil reports of Pueraria and Dolichites are reevaluated. • Pueraria had begun to diversify by at least the Middle Miocene and had spread into the Mio-Pliocene subtropical and temperate floras of the Balkan Peninsula, the Caucasus, and eastern Asia, which suggests the present diversity of this genus in tropical Asia and Oceania might have originated from the mid-latitudes of Eurasia.

  6. Parsing polyphyletic Pueraria: Delimiting distinct evolutionary lineages through phylogeny.

    PubMed

    Egan, Ashley N; Vatanparast, Mohammad; Cagle, William

    2016-11-01

    Several taxonomic and phylogenetic studies have hypothesized polyphyly within Pueraria DC., a genus comprising 19 species (24 with varieties) including the highly invasive Pueraria montana var. lobata (Kudzu) introduced to the U.S.A. about 150years ago. Previous efforts to investigate monophyly of the genus have been hampered by limited taxon sampling or a lack of comprehensive evolutionary context that would enable definitive taxonomic associations. This work presents a comprehensive phylogenetic investigation of Pueraria within the context of tribe Phaseoleae (Leguminosae). Polyphyly was found to be more extensive than previously thought, with five distinct lineages spread across the tribe and spanning over 25mya of divergence strongly supported by two chloroplast and one nuclear marker, AS2, presented here as a phylogenetic marker for the first time. Our phylogenies support taxonomic revisions to rectify polyphyly within Pueraria, including the resurrection of Neustanthus, moving one species to Teyleria, and the creation of two new genera, Haymondia and Toxicopueraria (taxonomic revisions published elsewhere). Published by Elsevier Inc.

  7. Urban and community forests of the Mountain region: Arizona, Colorado, Idaho, Montana, Nevada, New Mexico, Utah, Wyoming

    Treesearch

    David J. Nowak; Eric J. Greenfield

    2010-01-01

    This report details how land cover and urbanization vary within the states of Arizona, Colorado, Idaho, Montana, Nevada, New Mexico, Utah, and Wyoming by community (incorporated and census designated places), county subdivision, and county. Specifically this report provides critical urban and community forestry information for each state including human population...

  8. You grow where you're planted: Community building in Colstrip, Montana

    NASA Astrophysics Data System (ADS)

    Wilson, David Ramsey

    The expansion of energy production in the 1970s resulted in the construction of large extraction and power production facilities in many parts of the American West. Boomtowns almost always accompanied these enterprises. Colstrip, Montana, became the focus of a wide variety of social and environmental controversies when the Montana Power Company began strip mining operations and power plant construction in the early 1970s. Nevertheless, a sense of community attachment in Colstrip has steadily grown. Increased participation in public affairs, often in response to challenges made to the community, has accompanied the integration of Colstrip's residents in the non-economic environments of families, churches, recreation, and school-related activities. Researchers in the 1970s and early '80s often took the view that rapid development disrupts long-standing patterns of community attachment and integration. Using a model derived from Ferdinand Tonnies' Gemeinschaft-Gesell schaft continuum, these researchers undertook to demonstrate the folly of the energy companies' activities. The decline of community has frequently appeared as a theme in sociology and history. Yet the venerable but erroneous and largely sentimental theoretical perspective used by some early social impact assessment researchers did not accurately represent the processes at work in Colstrip and places like it. I suggest that Colstrip demonstrates an evolutionary continuum, but precisely the opposite of Tonnies' proposition. The feeling of attachment and home we call community is a growth-oriented phenomenon, not a simply a passive object subject only to decline. Colstrip, where sociologists found community lacking, is now found by the historian as the model of community.

  9. Community Background Reports: Cut Bank, Montana. National Study of American Indian Education, Series I, No. 8, Final Report.

    ERIC Educational Resources Information Center

    Humphrey, Theodore

    As a part of the Final Report of the National Study of American Indian Education, this paper is a report on the community of Cut Bank, Montana. Data were collected in the fall of 1968, when a 4-member team from the University of Colorado made a 6-week study at the Blackfeet Indian Reservation. General factors studied were location, physical…

  10. Recovery of big sagebrush communities after burning in south-western Montana.

    PubMed

    Wambolt, C L; Walhof, K S; Frisina, M R

    2001-03-01

    Prescribed burning of big sagebrush (Artemisia tridentata Nutt.) communities is conducted with the intention of increasing either the productivity of the understory plants or the big sagebrush. It was our objective to compare the recovery of big sagebrush communities from prescribed fire at as many sites as we could locate in south-western Montana with environmentally paired unburned portions. We located and sampled 13 sites that had been burned over a span of two to 32 growing seasons earlier. Big sagebrush canopy cover, density, and production of winter forage were significantly greater (P < or = 0.05) in the unburned portions in 34 of 38 comparisons. Canopy coverage of Idaho fescue (Festuca idahoensis Elmer), the dominant herbaceous species, was greater in the burned portion at only one site while it was less (P < or = 0.05) at four sites. Total perennial grass canopy coverage was not different (P < or = 0.05) between treatments over the 13 sites. Managers considering prescribed burning of big sagebrush communities should be aware that herbaceous plant responses may be minimal while shrub values will likely be lost for many years. The loss of the dominant shrubs in any ecosystem will affect many other organisms and severely impact species that have an obligate habit with the shrubs.

  11. Social Organization in Montana. Montana Economic Study-Staff Study.

    ERIC Educational Resources Information Center

    Bigart, Robert J.

    The four papers in this publication discusses Montana's social structure as it relates to culture, income, urbanism, and communal religious communities. "Montana Social Structure and Culture" includes rural and suburban life styles; the history of rural community organization; rural-small town communities; urban physical conditions;…

  12. Community Energy Systems and the Law of Public Utilities. Volume Twenty-eight. Montana

    SciTech Connect

    Feurer, D.A.; Weaver, C.L.

    1981-01-01

    A detailed description is presented of the laws and programs of the State of Montana governing the regulation of public energy utilities, the siting of energy generating and transmission facilities, the municipal franchising of public energy utilities, and the prescription of rates to be charged by utilities including attendant problems of cost allocations, rate base and operating expense determinations, and rate of return allowances. These laws and programs are analyzed to identify impediments which they may present to the implementation of Integrated Community Energy Systems (ICES). This report is one of fifty-one separate volumes which describe such regulatory programs at the Federal level and in each state as background to the report entitled Community Energy Systems and the Law of Public Utilities - Volume One: An Overview. This report also contains a summary of a strategy described in Volume One - An Overview for overcoming these impediments by working within the existing regulatory framework and by making changes in the regulatory programs to enhance the likelihood of ICES implementation.

  13. A primary assessment of the endophytic bacterial community in a xerophilous moss (Grimmia montana) using molecular method and cultivated isolates.

    PubMed

    Liu, Xiao Lei; Liu, Su Lin; Liu, Min; Kong, Bi He; Liu, Lei; Li, Yan Hong

    2014-01-01

    Investigating the endophytic bacterial community in special moss species is fundamental to understanding the microbial-plant interactions and discovering the bacteria with stresses tolerance. Thus, the community structure of endophytic bacteria in the xerophilous moss Grimmia montana were estimated using a 16S rDNA library and traditional cultivation methods. In total, 212 sequences derived from the 16S rDNA library were used to assess the bacterial diversity. Sequence alignment showed that the endophytes were assigned to 54 genera in 4 phyla (Proteobacteria, Firmicutes, Actinobacteria and Cytophaga/Flexibacter/Bacteroids). Of them, the dominant phyla were Proteobacteria (45.9%) and Firmicutes (27.6%), the most abundant genera included Acinetobacter, Aeromonas, Enterobacter, Leclercia, Microvirga, Pseudomonas, Rhizobium, Planococcus, Paenisporosarcina and Planomicrobium. In addition, a total of 14 species belonging to 8 genera in 3 phyla (Proteobacteria, Firmicutes, Actinobacteria) were isolated, Curtobacterium, Massilia, Pseudomonas and Sphingomonas were the dominant genera. Although some of the genera isolated were inconsistent with those detected by molecular method, both of two methods proved that many different endophytic bacteria coexist in G. montana. According to the potential functional analyses of these bacteria, some species are known to have possible beneficial effects on hosts, but whether this is the case in G. montana needs to be confirmed.

  14. Health-hazard evaluation report HETA 87-176-1826, St. James Community Hospital, Butte, Montana

    SciTech Connect

    Gunter, B.J.

    1987-08-01

    In response to a request from the Director of Nursing Service at St. James Community Hospital located in Butte, Montana, an evaluation was made of exposures to glutaraldehyde in the respiratory therapy and sigmoidoscopy departments of the hospital, where it was used in equipment sterilization. Glutaraldehyde concentrations in three breathing-zone samples were 0.25mg/cu m, 0.38mg/cu m, and below the detection level. Of six general area samples, five showed measurable concentrations ranging from 0.48 to 0.209mg/cu m. The American Conference of Governmental Industrial Hygienists has set a threshold-limit value of 0.7mg/cu m ceiling value for glutaraldehyde. The author concludes that no health hazard exists for glutaraldehyde in these operations. Informal interviews of technicians in the two departments did not reveal any health problems that could be attributed to glutaraldehyde exposure. Respiratory therapy equipment was cleaned in a hallway used as a passageway for other personnel. It is recommended that an improved arrangement be devised for cleaning respiratory equipment and that the area used for the purpose not be used as a hallway. The author also recommends that the ventilation system for the sigmoidoscope disinfecting operations be shared with other facilities, as it was very effective.

  15. The Story of Story Mill-A Montana Community Working to Restore Wetlands

    EPA Pesticide Factsheets

    Story Mill, a 55-acre site on the outskirts of Bozeman, Montana, has undergone several transformations in recent history. The place is virtually a “mill of stories” with respect to land use, but originally it was a wetland.

  16. Radiographic abnormalities and exposure to asbestos-contaminated vermiculite in the community of Libby, Montana, USA.

    PubMed

    Peipins, Lucy A; Lewin, Michael; Campolucci, Sharon; Lybarger, Jeffrey A; Miller, Aubrey; Middleton, Dan; Weis, Christopher; Spence, Michael; Black, Brad; Kapil, Vikas

    2003-11-01

    Mining, handling, processing, and personal or commercial use of asbestos-contaminated vermiculite have led to widespread contamination of the Libby, Montana, area. We initiated a medical testing program in response to reports of respiratory illness in the community. The purpose of this analysis was to identify and quantify asbestos-related radiographic abnormalities among persons exposed to vermiculite in Libby and to examine associations between these outcomes and participants' self-reported exposures. A cross-sectional interview and medical testing were conducted in Libby from July through November 2000 and from July through September 2001. A total of 7,307 persons who had lived, worked, or played in Libby for at least 6 months before 31 December 1990 completed the interview. Of those, 6,668 participants > or = 18 years of age received chest radiographs to assess the prevalence of pleural and interstitial abnormalities. We observed pleural abnormalities in 17.8% of participants and interstitial abnormalities in < 1% of participants undergoing chest radiography. We examined 29 occupational, recreational, household, and other exposure pathways in the analysis. The prevalence of pleural abnormalities increased with increasing number of exposure pathways, ranging from 6.7% for those who reported no apparent exposures to 34.6% for those who reported > or = 12 pathways. The factors most strongly associated with pleural abnormalities were being a former W.R. Grace worker, being older, having been a household contact of a W.R. Grace worker, and being a male. In addition to being a former W.R. Grace worker, environmental exposures and other nonoccupational risk factors were also important predictors of asbestos-related radiographic abnormalities.

  17. Radiographic abnormalities and exposure to asbestos-contaminated vermiculite in the community of Libby, Montana, USA.

    PubMed Central

    Peipins, Lucy A; Lewin, Michael; Campolucci, Sharon; Lybarger, Jeffrey A; Miller, Aubrey; Middleton, Dan; Weis, Christopher; Spence, Michael; Black, Brad; Kapil, Vikas

    2003-01-01

    Mining, handling, processing, and personal or commercial use of asbestos-contaminated vermiculite have led to widespread contamination of the Libby, Montana, area. We initiated a medical testing program in response to reports of respiratory illness in the community. The purpose of this analysis was to identify and quantify asbestos-related radiographic abnormalities among persons exposed to vermiculite in Libby and to examine associations between these outcomes and participants' self-reported exposures. A cross-sectional interview and medical testing were conducted in Libby from July through November 2000 and from July through September 2001. A total of 7,307 persons who had lived, worked, or played in Libby for at least 6 months before 31 December 1990 completed the interview. Of those, 6,668 participants > or = 18 years of age received chest radiographs to assess the prevalence of pleural and interstitial abnormalities. We observed pleural abnormalities in 17.8% of participants and interstitial abnormalities in < 1% of participants undergoing chest radiography. We examined 29 occupational, recreational, household, and other exposure pathways in the analysis. The prevalence of pleural abnormalities increased with increasing number of exposure pathways, ranging from 6.7% for those who reported no apparent exposures to 34.6% for those who reported > or = 12 pathways. The factors most strongly associated with pleural abnormalities were being a former W.R. Grace worker, being older, having been a household contact of a W.R. Grace worker, and being a male. In addition to being a former W.R. Grace worker, environmental exposures and other nonoccupational risk factors were also important predictors of asbestos-related radiographic abnormalities. PMID:14594627

  18. Plant community classification for alpine vegetation on the Beaverhead National Forest, Montana

    Treesearch

    Stephen V. Cooper; Peter Lesica; Deborah Page-Dumroese

    1997-01-01

    Vegetation of the alpine zone of eight mountain ranges in southwestern Montana was classified using IWINSPAN, DECORAN, and STRATA-algorithms embedded within the U.S. Forest Service Northern Region's ECADS (ecological classification and description system) program. Quantitative estimates of vegetation and soil attributes were sampled from 138 plots. Vegetation...

  19. Short- and longer-term effects of fire and herbivory on sagebrush communities in south-central Montana.

    PubMed

    Van Dyke, Fred; Darragh, Jeffrey A

    2006-09-01

    To better understand the role of herbivory and fire as potential disturbance processes in sagebrush communities, we examined responses of a grazing ungulate, elk (Cervus elaphus), following prescribed burning of sagebrush (Artemisia tridentata ssp. vaseyana) in south-central Montana (USA.) with concurrent monitoring of changes in plant production, nutritional quality, and community diversity from 1989-1999. Burning transformed low-diversity, sagebrush-dominated communities into high-diversity, graminoid-forb communities that persisted for 10 years without significant reestablishment of sagebrush. Elk increased use of burned sites one year after burning, but elk use returned to pre-burn levels over the next two to nine years. Forage biomass and nutritional quality declined after initial increases that coincided with increased elk use. Increases in elk use appeared to be influenced by increases in combined graminoid and forb production and changes in structural vegetation characteristics that permitted greater foraging efficiency. Declines in use were associated with loss of nutritional enhancement and declines in combined graminoid and forb production. Managers may observe only short-term responses from grazing ungulates to prescribed fire in sagebrush communities, but can expect longer-term increases in plant diversity and establishment of graminoid-forb communities.

  20. The future of European mining: What should our mining industry learn from the Skouries and Rosia Montana community gold conflicts

    NASA Astrophysics Data System (ADS)

    Hardy, Liam; Heller, Shaun; Faltyn, Rowan; Stefanaki, Anna; Economidou, Romina; Savin, Irina; Hood, Leo; Conway, Christopher

    2017-04-01

    The popular image of mining portrayed by media and by a majority of public opinion is a dominantly negative one. From worker's rights to environmental damages, disasters such as the Copiapó mine collapse (Chile), the acid mine drainage at Lousal (Portugal) and the Pb contamination of waters around the Tyndrum mines (Scotland) overshadow initiatives like the ICMM. Some companies receive little praise despite creating active community education and investment projects, while others simply build higher barbed wire fences and attempt to weather the protests, budgeting them into mine life assessments. This image problem, combined with the decentralised political segregation of Europe and the increased power of grass-roots protest initiatives (such as Antigold in Greece), has resulted in mining companies joining a long list of industries effected by the 'auto-protest' reaction in face of development, regardless of potential regional and national benefits, there is a pre-existing lack of trust in corporate and government powers to protect community interests. The poor management of existing licences is thus becoming a significant danger to future operations and the wider industry. Here we report on the Rosia Montana dispute (Romania) and the ongoing Skouries conflict (Greece). We then discuss how the European mining industry may need to significantly adapt its exploration and community engagement strategies to avoid future conflicts and, present a recent example of how effective suitably organised community engagement projects can be for local mining initiatives from Southern Portugal.

  1. Native American Vietnam-era Veterans' Access to VA Healthcare: Vulnerability and Resilience in Two Montana Reservation Communities.

    PubMed

    Ward, Carol J; Cope, Michael R; Elmont, Lindsey

    2017-03-23

    As a growing segment of the military, Native Americans are expected to increase enrollment in Department of Veterans Affairs (VA) healthcare. Currently, 20% of Native American veterans are aged 65-74, which means they served during the Vietnam era. This study explores the experiences of rural American Indian veterans from two Montana reservations with accessing VA health services. Utilizing detailed data obtained in focus group and individual interviews, we examine the experiences, attitudes, barriers and needs of rural Vietnam-era veterans. Analyses indicate that while Native American Vietnam-era veterans experienced a poor reception returning to the US after military service, they had more positive receptions in their home reservation communities. However, reintegration was often impeded by poor local opportunity structures and limited resources. As they have aged and turned to the VA for healthcare, these veterans have encountered barriers such as lack of information regarding eligibility and services, qualifying for care, excessive distances to health services, the cost of travel, and poor quality of assistance from VA personnel. Despite variations in their resources, tribal community efforts to honor veterans have begun to facilitate better access to healthcare. Focusing on the roles and importance of place-based resources, this study clarifies challenges and obstacles that Native American Vietnam-era veterans experience with accessing VA health services in rural, reservation communities. Additionally, findings show how tribal efforts are facilitating access as they begin to implement the 2010 agreement between the VA and Indian Health Services to better serve Native veterans.

  2. Muellerius capillaris dominates the lungworm community of bighorn sheep at the National Bison Range, Montana.

    PubMed

    Ezenwa, Vanessa O; Hines, Alicia M; Archie, Elizabeth A; Hoberg, Eric P; Asmundsson, Ingrid M; Hogg, John T

    2010-07-01

    Lungworm infections are common among bighorn sheep (Ovis canadensis) in North America, and the predominant species reported are Protostrongylus stilesi and P. rushi. The only records of another lungworm species, Muellerius capillaris, infecting bighorns come from South Dakota, USA. At the National Bison Range (NBR), Montana, USA we found that across six sampling periods, 100% of wild bighorn sheep surveyed were passing first-stage dorsal-spined larvae (DSL) which appeared to be consistent with M. capillaris. By contrast, only 39% or fewer sheep were passing Protostrongylus larvae. Using molecular techniques, we positively identified the DSL from the NBR bighorns as M. capillaris. This is the first definitive record of M. capillaris infection in a free-ranging bighorn sheep population outside of South Dakota.

  3. Comparison of macroinvertebrate community structure between two riffle-based sampling protocols in Wyoming, Colorado, and Montana, 2000-2001

    USGS Publications Warehouse

    Peterson, David A.; Zumberge, Jeremy R.

    2006-01-01

    Samples of benthic macroinvertebrates were collected side-by-side from riffles at 12 stream sites in Wyoming, Colorado, and Montana during 2000-2001, following protocols established by the U.S. Geological Survey National Water-Quality Assessment (NAWQA) Program and the U.S. Environmental Protection Agency Environmental Monitoring and Assessment Program (EMAP). Samples from riffles were collected following NAWQA protocols, using a sampler with 425-micron net mesh-opening size from a total area of 1.25 m2 per sample in multiple riffles. Samples also were collected following EMAP protocols, using a sampler with 500-micron net mesh-opening size from a total area of 0.72 m2 per sample in multiple riffles. The taxonomic identification and enumeration of the samples followed procedures established for each program. Benthic macroinvertebrate community structure was compared between the data sets using individual metrics, a multimetric index, and multivariate analysis. Comparisons between the macroinvertebrate community structures were made after sequentially adjusting both data sets for: (1) ambiguous taxa, (2) taxonomic inconsistencies, and (3) differences in laboratory subsampling. After removal of ambiguous taxa, pair-wise differences in total taxa richness and Ephemeroptera taxa richness were statistically significant (p < 0.05). Differences between the data sets generally were not significant for richness of other taxa, tolerant taxa, semi-voltine taxa, functional feeding groups, diversity, and dominance. Sample scores calculated using the Wyoming Stream Integrity Index were not significantly different between the two data sets. After reconciling both data sets for taxonomic inconsistencies, total taxa richness and Ephemeroptera taxa richness remained significantly different between the data sets. After adjusting the data for differences in laboratory subsampling, the differences in taxa richness were no longer significant. Bray-Curtis similarity coefficients and non

  4. Montana Wildfires

    Atmospheric Science Data Center

    2014-05-15

    article title:  Montana Wildfires     View larger image ... in the early summer of 2012 has been on the destructive wildfires in Colorado, as of July 3, 2012, dozens of major wildfires were burning across the western United States, including six in ...

  5. A new isoflavone glycoside from Pueraria alopecuroides.

    PubMed

    Yang, Junlin; Fan, Qingfei; Zhang, Huanli; Song, Qishi

    2016-01-01

    A new isoflavone glycoside, (-)-tuberosin-3-O-β-D-glucopyranoside (1), along with 10 known compounds 1a-10, was isolated from Pueraria alopecuroides. Their structures were determined on the basis of spectral data including 1D and 2D NMR and HREIMS. These compounds were isolated from this plant for the first time.

  6. Community Support for a Gold Cyanide Process Mine: Resident and Leader Differences in Rural Montana

    ERIC Educational Resources Information Center

    Richards, Rebecca T.; Brod, Rodney L.

    2004-01-01

    Previous studies have established that community residents and leaders differ in their support for hazardous waste facility siting in rural areas (Spies et al. 1998). We examine whether these same differences exist in rural communities that face other high-risk development decisions by analyzing resident and leader support for a proposed gold…

  7. Community Support for a Gold Cyanide Process Mine: Resident and Leader Differences in Rural Montana

    ERIC Educational Resources Information Center

    Richards, Rebecca T.; Brod, Rodney L.

    2004-01-01

    Previous studies have established that community residents and leaders differ in their support for hazardous waste facility siting in rural areas (Spies et al. 1998). We examine whether these same differences exist in rural communities that face other high-risk development decisions by analyzing resident and leader support for a proposed gold…

  8. Workforce: Montana

    ERIC Educational Resources Information Center

    Western Interstate Commission for Higher Education, 2006

    2006-01-01

    Employment in Montana (including hourly and salaried jobs and self-employment) is projected to grow by 17 percent from 2002 to 2012, adding over 96,000 new jobs to the state's economy and growing the workforce from 554,456 to 651,135. The rate of growth is higher than the 15 percent increase projected for the nation as a whole. Growth will occur…

  9. Metal and arsenic impacts to soils, vegetation communities and wildlife habitat in southwest Montana uplands contaminated by smelter emissions. 1: Field evaluation

    SciTech Connect

    Galbraith, H.; LeJeune, K.; Lipton, J.

    1995-11-01

    Concentrations of arsenic and metals in soils surrounding a smelter in southwest Montana were correlated with vegetative community structure and composition and wildlife habitat quality. Soils in the uplands surrounding the smelter were highly enriched with arsenic and metals. Concentrations of these analytes decreased with distance from the smelter and with soil depth, suggesting that the smelter is the source of the enrichment. In enriched areas, marked modifications to the native vegetation community structure and composition were observed. These included replacement of evergreen forest with bare unvegetated ground; species impoverishment and increased dominance by weed species in grasslands; and reductions in the vertical complexity of the habitat. Significant negative correlations existed between soil arsenic and metals concentrations and the extent of vegetative cover and the vertical diversity of plant communities. Loss of vegetative cover in the affected areas has been accompanied by reductions in their capacity to support indigenous wildlife populations.

  10. Major isoflavonoid contents of the phytoestrogen rich-herb Pueraria mirifica in comparison with Pueraria lobata.

    PubMed

    Cherdshewasart, Wichai; Subtang, Subongkoj; Dahlan, Winai

    2007-01-17

    Pueraria mirifica tubers collected from 28 out of 76 provinces of Thailand and Pueraria lobata tubers collected from Guangzhou province, China were submitted to HPLC analysis with the established gradient system comprising 1.5% acetic acid and acetonitrile. Five major isoflavonoids, including puerarin, daidzin, genistin, daidzein and genistein, were adopted as authentic standards. P. mirifica tubers showed intra- as well as inter-provincial differences in isoflavonoid and total isoflavonoid contents. The difference in both cases should be mostly influenced by genetic and environmental factors. In comparison with P. lobata, P. mirifica population exhibited differences only with a lower amount of daidzein.

  11. [Preventive effects of pueraria on presbycusis in rats].

    PubMed

    Chen, Wangyan; Yao, Qi; Liu, Weihong; Zhang, Bibo; Wang, Ying; Liu, Bo

    2009-08-01

    To investigate the preventive effects of Pueraria on presbycusis in rats. Thirty-two 24-26 month old Wistar rats were randomly divided into four groups, and were treated with different dosages of Pueraria (1, 2, 4, 0 g x kg(-1) x d(-1)) separately for 4 weeks. Auditory brainstem response (ABR) was used to detect the change of hearing threshold of rats. Hemorheological items of rats were checked in each group. Compared with control group, the hearing threshold and hemorheological items of rats was significantly improved after treated with Pueraria (P<0.05). In addition, 2 g/(kg x d) was found to be the best dosage of Pueraria for rats, which can achieve ideal effect with minimum side effect. Pueraria could improve tiny circulation, has good preventive effect on presbycusis of rats.

  12. Differentiating Puerariae Lobatae Radix and Puerariae Thomsonii Radix using HPTLC coupled with multivariate classification analyses.

    PubMed

    Wong, Ka H; Razmovski-Naumovski, Valentina; Li, Kong M; Li, George Q; Chan, Kelvin

    2014-07-01

    Puerariae Lobatae Radix (PLR), the root of Pueraria lobata, is a traditional Chinese medicine for treating diabetes and cardiovascular diseases. Puerariae Thomsonii Radix (PTR), the root of Pueraria thomsonii, is a closely related species to PLR and has been used as a PLR substitute in clinical practice. The aim of this study was to compare the classification accuracy of high performance thin-layer chromatography (HPTLC) with that of ultra-performance liquid chromatography (UPLC) in differentiating PLR from PTR. The Matlab functions were used to facilitate the digitalisation and pre-processing of the HPTLC plates. Seven multivariate classification methods were evaluated for the two chromatographic methods. The results demonstrated that the HPTLC classification models were comparable to the UPLC classification models. In particular, k-nearest neighbours, partial least square-discriminant analysis, principal component analysis-discriminant analysis and support vector machine-discriminant analysis showed the highest rate of correct species classification, whilst the lowest classification rate was obtained from soft independent modelling of class analogy. In conclusion, HPTLC combined with multivariate analysis is a promising technique for the quality control and differentiation of PLR and PTR. Copyright © 2014 Elsevier B.V. All rights reserved.

  13. A Project to Promote Adherence to Blood Pressure Medication Among People Who Use Community Pharmacies in Rural Montana, 2014–2016

    PubMed Central

    Fogle, Crystelle C.; Bennett, James A.

    2017-01-01

    Introduction Pharmacists can assist patients in managing their blood pressure levels. We assessed whether adherence to blood pressure medication improved among people who used community pharmacies in rural Montana after pharmacists initiated consultations and distributed educational materials developed for the Million Hearts Initiative’s “Team Up. Pressure Down.” (TUPD) program. Methods From 2014 to 2016, the Cardiovascular Health Program at the Montana Department of Public Health and Human Services conducted a statewide project to evaluate an intervention for adherence to blood pressure medication administered through community pharmacies. After the year 1 pilot, we redesigned the program for year 2 and year 3 and measured the percentage of participating patients who adhered to blood pressure medication. We also conducted a statewide survey to assess pharmacy characteristics, computer-system capabilities, and types of consulting services provided by pharmacists. Results Twenty-five community pharmacies completed Montana’s TUPD program: 8 pharmacies in the pilot year, 11 pharmacies in year 2, and 6 pharmacies in year 3. For year 2 and year 3 combined, the percentage of participating patients who achieved blood pressure medication adherence improved preintervention to postintervention from 73% to 89%, and adherence improved in 15 of the 17 pharmacies. The pilot pharmacies identified 3 major barriers to project success: patient buy-in, staff burden in implementing the project, and funding. In the statewide assessment, TUPD-funded pharmacies were significantly more likely than non-TUPD–funded pharmacies to provide prescription synchronization and medication management with feedback to the patient’s physician. Conclusion Community pharmacies in rural areas can effectively use brief consultations and standard educational materials to improve adherence to blood pressure medication. PMID:28662759

  14. Assessment of potential effects of water produced from coalbed natural gas development on macroinvertebrate and algal communities in the Powder River and Tongue River, Wyoming and Montana, 2010

    USGS Publications Warehouse

    Peterson, David A.; Hargett, Eric G.; Feldman, David L.

    2011-01-01

    Ongoing development of coalbed natural gas in the Powder River structural basin in Wyoming and Montana led to formation of an interagency aquatic task group to address concerns about the effects of the resulting production water on biological communities in streams of the area. Ecological assessments, made from 2005–08 under the direction of the task group, indicated biological condition of the macroinvertebrate and algal communities in the middle reaches of the Powder was lower than in the upper or lower reaches. On the basis of the 2005–08 results, sampling of the macroinvertebrate and algae communities was conducted at 18 sites on the mainstem Powder River and 6 sites on the mainstem Tongue River in 2010. Sampling-site locations were selected on a paired approach, with sites located upstream and downstream of discharge points and tributaries associated with coalbed natural gas development. Differences in biological condition among site pairs were evaluated graphically and statistically using multiple lines of evidence that included macroinvertebrate and algal community metrics (such as taxa richness, relative abundance, functional feeding groups, and tolerance) and output from observed/expected (O/E) macroinvertebrate models from Wyoming and Montana. Multiple lines of evidence indicated a decline in biological condition in the middle reaches of the Powder River, potentially indicating cumulative effects from coalbed natural gas discharges within one or more reaches between Flying E Creek and Wild Horse Creek in Wyoming. The maximum concentrations of alkalinity in the Powder River also occurred in the middle reaches. Biological condition in the upper and lower reaches of the Powder River was variable, with declines between some site pairs, such as upstream and downstream of Dry Fork and Willow Creek, and increases at others, such as upstream and downstream of Beaver Creek. Biological condition at site pairs on the Tongue River showed an increase in one case

  15. High-performance thin-layer chromatographic fingerprints of isoflavonoids for distinguishing between Radix Puerariae Lobate and Radix Puerariae Thomsonii.

    PubMed

    Chen, Si-Bao; Liu, He-Ping; Tian, Run-Tao; Yang, Da-Jian; Chen, Shi-Lin; Xu, Hong-Xi; Chan, Albert S C; Xie, Pei-Shan

    2006-07-14

    The roots of Pueraria lobata (Wild.) Ohwi and Pueraria thomsonii Benth have been officially recorded in all editions of Chinese Pharmacopoeia under the same monograph 'Gegen' (Radix Puerariae, RP). However, in its 2005 edition, the two species were separated into both individual monographs, namely 'Gegen' (Radix Puerariae Lobatae, RPL) and 'Fenge' (Radix Puerariae Thomsonii, RPT), respectively, due to their obvious content discrepancy of puerarin, the major active constituent. In present paper, the fingerprint of high-performance thin-layer chromatography (HPTLC) combining digital scanning profiling was developed to identify and distinguish the both species in detail. The unique properties of the HPTLC fingerprints were validated by analyzing ten batches of Pueraria lobata and P. thomsonii samples, respectively. The common pattern of the HPTLC images of the roots of Pueraria spp. and the respective different ratios of the chemical distribution can directly discern the two species. The corresponding digital scanning profiles provided an easy way for quantifiable comparison among the samples. Obvious difference in ingredient content and HPTLC patterns of the two species questioned their bio-equivalence and explained that recording both species separately in the current edition of Chinese Pharmacopoeia (2005 edition) is reasonable due to not only the content of major constituent, puerarin, but also the peak-to-peak distribution in the fingerprint and integration value of the total components. Furthermore, the HPTLC fingerprint is also suitable for rapid and simple authentication and comparison of the subtle difference among samples with identical plant resource but different geographic locations.

  16. Montana Logging Utilization, 2002

    Treesearch

    Todd A. Morgan; Timothy P. Spoelma; Charles E. Keegan; Alfred L. Chase; Michael T. Thompson

    2005-01-01

    A study of logging utilization in Montana during 2002 provided logging and product utilization data for sawlog and veneer log harvests in Montana. Results of the study indicate a shift toward greater utilization of smaller diameter material, as 78 percent of the harvested volume in Montana during 2002 came from trees less than 17 inches diameter at breast height. The...

  17. Seasonal variations in the isoflavonoids of radix Puerariae.

    PubMed

    Sibao, Chen; Dajian, Yang; Shilin, Chen; Hongx, Xu; Chan, Albert S C

    2007-01-01

    The variations in isoflavonoid content in Radix Puerariae, the root of Pueraria lobata (Wild) Ohwi, have been examined in order to determine the optimum time to harvest the herb. Total isoflavonoid contents have been analysed by a UV spectrophotometric method whilst HPLC with photodiode array detection has been used to monitor the contents of seven major individual isoflavonoids, namely, 3'-hydroxypuerarin, puerarin, 3'-methoxypuerarin, daidzin, genistin, formononetin-7-glucoside and daidzein. Ninety-six samples of Radix Puerariae of different ages and harvested in different months were collected and analysed. The results clearly showed that 3-year-old roots harvested in January have the highest yields of isoflavonoid compounds. Moreover, the results obtained by both methods have a strong correlation. This suggested that the simple and fast UV spectrophotometric method could be used for monitoring the phytochemical quality of Radix Puerariae.

  18. Differentiation of Pueraria lobata and Pueraria thomsonii using partial least square discriminant analysis (PLS-DA).

    PubMed

    Wong, Ka H; Razmovski-Naumovski, Valentina; Li, Kong M; Li, George Q; Chan, Kelvin

    2013-10-01

    The aims of the study were to differentiate Pueraria lobata from its related species Pueraria thomsonii and to examine the raw herbal material used in manufacturing kudzu root granules using partial least square discriminant analysis (PLS-DA). Sixty-four raw materials of P. lobata and P. thomsonii and kudzu root-labelled granules were analysed by ultra performance liquid chromatography. To differentiate P. lobata from P. thomsonii, PLS-DA models using the variables selected from the entire chromatograms, genetic algorithm (GA), successive projection algorithm (SPA), puerarin alone and six selected peaks were employed. The models constructed by GA and SPA demonstrated superior classification ability and lower model's complexity as compared to the model based on the entire chromatographic matrix, whilst the model constructed by the six selected peaks was comparable to the entire chromatographic model. The model established by puerarin alone showed inferior classification ability. In addition, the PLS-DA models constructed by the entire chromatographic matrix, GA, SPA and the six selected peaks showed that four brands out of seventeen granules were mislabelled as P. lobata. In conclusion, PLS-DA is a promising procedure for differentiating Pueraria species and determining raw material used in commercial products.

  19. Challenges and Sustainability Practices of Frontier Schools in Montana

    ERIC Educational Resources Information Center

    Morton, Claudette; Harmon, Hobart L.

    2011-01-01

    This article reports the findings of a study commissioned by the Montana Small Schools Alliance to explore the challenges and sustainability practices of frontier schools. A Montana frontier school is defined as a school district with 200 or fewer students with its attendant community located in a county with five or fewer people per square mile.…

  20. Effects of Agaricus lilaceps fairy rings on soil aggregation and microbial community structure in relation to growth stimulation of western wheatgrass (Pascopyrum smithii) in Eastern Montana rangeland.

    PubMed

    Caesar-Tonthat, The Can; Espeland, Erin; Caesar, Anthony J; Sainju, Upendra M; Lartey, Robert T; Gaskin, John F

    2013-07-01

    Stimulation of plant productivity caused by Agaricus fairy rings has been reported, but little is known about the effects of these fungi on soil aggregation and the microbial community structure, particularly the communities that can bind soil particles. We studied three concentric zones of Agaricus lilaceps fairy rings in Eastern Montana that stimulate western wheatgrass (Pascopyrum smithii): outside the ring (OUT), inside the ring (IN), and stimulated zone adjacent to the fungal fruiting bodies (SZ) to determine (1) soil aggregate proportion and stability, (2) the microbial community composition and the N-acetyl-β-D-glucosaminidase activity associated with bulk soil at 0-15 cm depth, (3) the predominant culturable bacterial communities that can bind to soil adhering to wheatgrass roots, and (4) the stimulation of wheatgrass production. In bulk soil, macroaggregates (4.75-2.00 and 2.00-0.25 mm) and aggregate stability increased in SZ compared to IN and OUT. The high ratio of fungal to bacteria (fatty acid methyl ester) and N-acetyl-β-D-glucosaminidase activity in SZ compared to IN and OUT suggest high fungal biomass. A soil sedimentation assay performed on the predominant isolates from root-adhering soil indicated more soil-binding bacteria in SZ than IN and OUT; Pseudomonas fluorescens and Stenotrophomonas maltophilia isolates predominated in SZ, whereas Bacillus spp. isolates predominated in IN and OUT. This study suggests that growth stimulation of wheatgrass in A. lilaceps fairy rings may be attributed to the activity of the fungus by enhancing soil aggregation of bulk soil at 0-15 cm depth and influencing the amount and functionality of specific predominant microbial communities in the wheatgrass root-adhering soil.

  1. Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using ¹H NMR spectroscopy and multivariate statistical analysis.

    PubMed

    Chen, Yan-Gan; Song, Yue-Lin; Wang, Ying; Yuan, Yun-Fei; Huang, Xiao-Jun; Ye, Wen-Cai; Wang, Yi-Tao; Zhang, Qing-Wen

    2014-05-01

    Puerariae Radix was a widely used herbal medicine. Pueraria lobata (PL) and Pueraria thomsonii (PT) were the two authorized sources of Puerariae Radix (gegen) in China. In this study, metabolic differentiations between these two species were investigated using NMR spectroscopy followed by principal components analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). The content of puerarin in PL and PT was also determined using quantitative (1)H NMR spectroscopy. Thirteen isoflavones were tentatively identified based on 1D and 2D NMR spectroscopic data in two species. The (1)H NMR spectra of PL and PT were obviously different. PL and PT could also be markedly discriminated from (1)H NMR spectroscopic data by PCA and PLS-DA. For the crude drug resources, isoflavones, in which puerarin is the most important one, were regarded as the reasonable markers for the discrimination of the two species. The contents of puerarin and total isoflavones in PL were quantitated much higher than those in PT. Above all, (1)H NMR spectroscopy, which can provide comprehensive profiles of the metabolites and achieve convenient determinations of puerarin and total isoflavones in a single run, is an efficient means for evaluating the medicinal samples and achieving a better quality control of Puerariae Radix. Copyright © 2013 Elsevier B.V. All rights reserved.

  2. Select mortality and cancer incidence among residents in various U.S. communities that received asbestos-contaminated vermiculite ore from Libby, Montana.

    PubMed

    Horton, D Kevin; Bove, Frank; Kapil, Vikas

    2008-06-01

    In response to the significantly elevated asbestosis mortality rates found in Libby, Montana, in 2000, this analysis evaluated whether other communities throughout the United States that received asbestos-contaminated vermiculite ore from Libby experienced similar excess rates of asbestos-related diseases. Standardized mortality ratios were calculated using state death certificates, and standardized incidence ratios were calculated using cancer registry records for populations living near facilities that processed or received Libby vermiculite. This analysis focused primarily on diseases that are directly associated with asbestos exposure (e.g., asbestosis; cancer of the peritoneum, retroperitoneum, and pleura, including mesothelioma; and mesothelioma). Lung cancer and cancers of the digestive system, also associated with asbestos exposure, were not included in the analysis because they have additional risk factors for which exposure information was not available. Data from 70 unique sites in 23 states were evaluated. No statistically significant excesses of asbestosis mortality similar to those in Libby were noted; however, 11 sites (plus a state with 6 pooled sites that were counted as 1 site) had excess rates of mesothelioma and cancer of the peritoneum, retroperitoneum, and pleura. Further investigation should be conducted at these sites with excess rates of mesothelioma and cancer of the peritoneum, retroperitoneum, and pleura by participating state health departments to determine whether exposure to Libby vermiculite might have been a contributing factor.

  3. Metal and arsenic impacts to soils, vegetation communities and wildlife habitat in southwest Montana uplands contaminated by smelter emissions. 2: Laboratory phytotoxicity studies

    SciTech Connect

    Kapustka, L.A.; Lipton, J.; Galbraith, H.; Cacela, D.; LeJeune, K.

    1995-11-01

    Vegetation communities on metal- and arsenic-contaminated uplands surrounding a smelter in southwest Montana have been eliminated or highly modified. Laboratory toxicity tests were performed using site soils from the impacted areas to determine whether the soils limit the ability of plants to establish and grow. The germination and growth of alfalfa, lettuce, and wheat in impacted area soils was compared to germination and growth of the three species in reference soils. The degree of phytotoxicity was quantified using a species-endpoint toxicity score calculated on the magnitude of difference between germination and growth of plants in impacted and reference soils. The impacted soils exhibited substantial toxicity to plants: 5% of the sites were severely phytotoxic, 55% were highly phytotoxic, 10% were moderately phytotoxic, 20% were mildly phytotoxic, and 10% were nontoxic. Root growth was consistently the most affected endpoint (18 of 20 impacted soils) and reduction in root length and mass was observed. Correlation and partial correlation analysis was used to evaluate the causes of phytotoxicity. Concentrations of As, Cu, and Zn and, to a lesser extent, Pb and Cd were found to be positively correlated with phytotoxicity.

  4. Community-Level Social Support Responses in a Slow-Motion Technological Disaster: The Case of Libby, Montana

    PubMed Central

    Orom, Heather; Berry-Bobovski, Lisa; Hernandez, Tanis; Black, C. Brad; Schwartz, Ann G.; Ruckdeschel, John C.

    2013-01-01

    Social support is an important resource for communities experiencing disasters. However, a disaster's nature (rapid- versus slow-onset, natural versus technological) may influence community-level responses. Disaster research on social support focuses primarily on rapid-onset natural disasters and, to a lesser extent, rapid-onset technological disasters. Little research has addressed slow-onset disasters. This study explores social support processes in Libby, MT, a community experiencing a “slow-motion technological disaster” due to widespread amphibole asbestos exposure. A comprehensive social support coding system was applied to focus-group and in-depth-interview transcripts. Results reveal that, although the community has a history of normative supportiveness during community and individual crises, that norm has been violated in the asbestos disaster context. Results are interpreted as a failure to achieve an “emergent altruistic community.” Specifically, community-level conflict appears to interfere with previously established social support patterns. The observed phenomenon can be understood as the deterioration of a previously supportive community. PMID:20526664

  5. Community-level social support responses in a slow-motion technological disaster: the case of Libby, Montana.

    PubMed

    Cline, Rebecca J W; Orom, Heather; Berry-Bobovski, Lisa; Hernandez, Tanis; Black, C Brad; Schwartz, Ann G; Ruckdeschel, John C

    2010-09-01

    Social support is an important resource for communities experiencing disasters. However, a disaster's nature (rapid- versus slow-onset, natural versus technological) may influence community-level responses. Disaster research on social support focuses primarily on rapid-onset natural disasters and, to a lesser extent, rapid-onset technological disasters. Little research has addressed slow-onset disasters. This study explores social support processes in Libby, MT, a community experiencing a "slow-motion technological disaster" due to widespread amphibole asbestos exposure. A comprehensive social support coding system was applied to focus-group and in-depth-interview transcripts. Results reveal that, although the community has a history of normative supportiveness during community and individual crises, that norm has been violated in the asbestos disaster context. Results are interpreted as a failure to achieve an "emergent altruistic community." Specifically, community-level conflict appears to interfere with previously established social support patterns. The observed phenomenon can be understood as the deterioration of a previously supportive community.

  6. Rodent-vegetation relationships in southeastern Montana

    Treesearch

    James G. MacCracken; Daniel W. Uresk; Hansen; Richard M.

    1985-01-01

    Plant communities of southeastern Montana were surveyed for rodents over a two year period. Deer mice (Peromyscus maniculatus) were the most abundant rodent species found on the study area. Prairie voles (Microtus ochrogaster), meadow voles (M. pennsylvanicus), sagebrush voles (Lagurus curtatus...

  7. Montana StreamStats

    USGS Publications Warehouse

    2016-04-05

    About this volumeMontana StreamStats is a Web-based geographic information system (http://water.usgs.gov/osw/streamstats/) application that provides users with access to basin and streamflow characteristics for gaged and ungaged streams in Montana. Montana StreamStats was developed by the U.S. Geological Survey (USGS) in cooperation with the Montana Departments of Transportation, Environmental Quality, and Natural Resources and Conservation. The USGS Scientific Investigations Report consists of seven independent but complementary chapters dealing with various aspects of this effort.Chapter A describes the Montana StreamStats application, the basin and streamflow datasets, and provides a brief overview of the streamflow characteristics and regression equations used in the study. Chapters B through E document the datasets, methods, and results of analyses to determine streamflow characteristics, such as peak-flow frequencies, low-flow frequencies, and monthly and annual characteristics, for USGS streamflow-gaging stations in and near Montana. The StreamStats analytical toolsets that allow users to delineate drainage basins and solve regression equations to estimate streamflow characteristics at ungaged sites in Montana are described in Chapters F and G.

  8. Comparing morphological, chemical and anti-diabetic characteristics of Puerariae Lobatae Radix and Puerariae Thomsonii Radix.

    PubMed

    Wong, Ka H; Razmovski-Naumovski, Valentina; Li, Kong M; Li, George Q; Chan, Kelvin

    2015-04-22

    Puerariae Lobatae Radix (PLR) and Puerariae Thomsonii Radix (PTR) are traditional Chinese medicines used for the treatment of diabetes and cardiovascular diseases. These two herbs are used interchangeably in clinical practice, even though they possess significantly different chemical profiles. In the case of Pueraria species, the misidentification is related to the multiple Chinese common names in clinical practice and variable pharmaceutical Latin names in different versions of the Pharmacopoeia of the People's Republic of China. In addition, there is lack of evidence demonstrating how the differences in the chemical profile would impact on the pharmacological activity of the two herbs. Therefore, the aim of this study was to compare the microscopic, phytochemical profiles and anti-diabetic activity of PLR and PTR so that the two species can be differentiated. The microscopic characteristics of the PLR and PTR were observed and measured by an optical microscope. The major compounds were quantified by ultra-performance liquid chromatography (UPLC) and total flavonoid content (TFC) colorimetric assay. The free radical scavenging capacity was assessed by 2,2-diphenyl-2-picrylhydrazyl (DPPH) antioxidant assays. Anti-diabetic activity was determined by the inhibition of porcine pancreatic α-amylase and rat intestinal α-glucosidase activities. Microscopic results illustrated that the size of xylem vessels (PLR: 0.1390 ± 0.0184 mm; PTR: 0.0471 ± 0.0109 mm), number of fibre per bundle (PLR: 32.6800 ± 2.8780; PTR: 16.5900 ± 0.9982) and the size of fibre (PLR: 0.0075 ± 0.0003 mm(2); PTR: 0.0025 ± 0.0002 mm(2)) in PLR were significantly greater than that in PTR (p<0.01). PLR possessed a significantly lower total starch content (PLR: 0.5288 ± 1.2559 mg starch/g DM; PTR: 76.7954 ± 2.9905 mg starch/g DM) and total dietary fibre content (PLR: 4.2886 ± 0.3466 g/100g DM; PTR: 12.4148 ± 0.4541 g/100g DM) as compared to PTR. Isoflavonoids including puerarin, daidzin

  9. Idaho-Montana Logging

    NASA Image and Video Library

    2013-12-16

    Logging operations have left a striking checkerboard pattern in the landscape along the Idaho-Montana border, sandwiched between Clearwater and Bitterroot National Forests as seen in this image acquired by NASA Terra spacecraft.

  10. Effects of Myrothecium verrucaria on ultrastructural integrity of kudzu (Pueraria montana var. lobata) and phytotoxin implications

    USDA-ARS?s Scientific Manuscript database

    The fungus Myrothecium verrucaria (Alb. & Schwein.) (MV), originally isolated from diseased sicklepod (Senna obtusifolia L.), has bioherbicial activity against kudzu and several other weeds when applied with low concentrations of the surfactant Silwet L-77. Ultrastructural investigations of MV effe...

  11. Invasive Species Biology, Control, and Research. Part 1: Kudzu (Pueraria montana)

    DTIC Science & Technology

    2008-11-01

    3 Reproduction ...overtop trees and surrounding vegetation. Reproduction Kudzu reportedly reproduces readily through vegetative spread (Foresth and Innis 2004...Vegetative reproduction occurs when stems come into contact with the ground, roots will develop at stem nodes. When plants senesce or stems are broken due

  12. Carbon and Nitrogen Levels across Forest Soil Communities Impacted by Bark Beetle and Wildfire Disturbance in Western Montana

    NASA Astrophysics Data System (ADS)

    Kim, E. S. M.; Ballantyne, A. P.; Cooper, L. A.; Hursh, A.

    2014-12-01

    Global climate change has had extensive impacts on the forest ecosystems of the western US, namely by causing increases in mountain pine beetle numbers and wildfires. Mountain pine beetles experience higher survival rates due to milder winters, allowing for greater frequency and severity of attacks and in turn causing more widespread pine tree mortality. Meanwhile, the arid conditions created by this temperature increase have been conducive to a surge in wildfires. Although many investigations have been carried out on the soil biogeochemistry in areas hit by one or the other, no study to our knowledge has explicitly researched the compound effects of these disturbances. This study examined soil levels of carbon (C) and nitrogen (N) as well as the C/N ratios of pine and fir soil communities that have been affected by both beetle and fire disturbance. Our results show that no significant differences were found in the C/N ratios in response to all modes of disturbance. However, significant C losses from the O horizon, but not the M horizon, were observed following wildfire. Similarly, losses in N from just the O horizon were observed, but these were not significant. In conclusion, fire resulted in marked declines in soil C, and forests impacted by beetle infestation and fire experienced C losses similar to fire alone.

  13. Pueraria lobata (Kudzu root) hangover remedies and acetaldehyde-associated neoplasm risk.

    PubMed

    McGregor, Neil R

    2007-11-01

    Recent introduction of several commercial Kudzu root (Pueraria lobata) containing hangover remedies has occurred in western countries. The available data is reviewed to assess if there are any potential concerns in relationship to the development of neoplasm if these products are used chronically. The herb Pueraria has two components that are used as traditional therapies; Pueraria lobata, the root based herb and Pueraria flos, the flower based herb. Both of these herbal components have different traditional claims and constituents. Pueraria flos, which enhances acetaldehyde removal, is the traditional hangover remedy. Conversely, Pueraria lobata is a known inhibitor of mitochondrial aldehyde dehydrogenase (ALDH2) and increases acetaldehyde. Pueraria lobata is being investigated for use as an aversion therapy for alcoholics due to these characteristics. Pueraria lobata is not a traditional hangover therapy yet has been accepted as the registered active component in many of these hangover products. The risk of development of acetaldehyde pathology, including neoplasms, is associated with genetic polymorphism with enhanced alcohol dehydrogenase (ADH) or reduced ALDH activity leading to increased acetaldehyde levels in the tissues. The chronic usage of Pueraria lobata at times of high ethanol consumption, such as in hangover remedies, may predispose subjects to an increased risk of acetaldehyde-related neoplasm and pathology. The guidelines for Disulfiram, an ALDH2 inhibitor, provide a set of guidelines for use with the herb Pueraria lobata. Pueraria lobata appears to be an inappropriate herb for use in herbal hangover remedies as it is an inhibitor of ALDH2. The recommendations for its use should be similar to those for the ALDH2 inhibitor, Disulfiram.

  14. Rapid analysis of Radix puerariae by near-infrared spectroscopy.

    PubMed

    Lau, Ching-Ching; Chan, Chi-On; Chau, Foo-Tim; Mok, Daniel Kam-Wah

    2009-03-13

    A new, rapid analytical method using near-infrared spectroscopy (NIRS) was developed to differentiate two species of Radix puerariae (GG), Pueraria lobata (YG) and Pueraria thomsonii (FG), and to determine the contents of puerarin, daidzin and total isoflavonoid in the samples. Five isoflavonoids, puerarin, daidzin, daidzein, genistin and genistein were analyzed simultaneously by high-performance liquid chromatography-diode array detection (HPLC-DAD). The total isoflavonoid content was exploited as critical parameter for successful discrimination of the two species. Scattering effect and baseline shift in the NIR spectra were corrected and the spectral features were enhanced by several pre-processing methods. By using linear discriminant analysis (LDA) and soft independent modeling class analogy (SIMCA), samples were separated successfully into two different clusters corresponding to the two GG species. Furthermore, sensitivity and specificity of the classification models were determined to evaluate the performance. Finally, partial least squares (PLS) regression was used to build the correlation models. The results showed that the correlation coefficients of the prediction models are R=0.970 for the puerarin, R=0.939 for daidzin and R=0.969 for total isoflavonoid. The outcome showed that NIRS can serve as routine screening in the quality control of Chinese herbal medicine (CHM).

  15. Geology of northeastern Montana

    USGS Publications Warehouse

    Collier, Arthur J.

    1919-01-01

    A large region in northeastern Montana has never been thoroughly explored by geologists, owing to the fact that it is a part of the Great Plains and the belief that it is too monotonous and uninteresting to tempt anyone to turn aside from the pronounced geologic features a little farther west, for which Montana is noted. This region includes parts of Sheridan, Valley, Phillips, and Blaine counties. Its investigation was begun by Smith in 1908, when he made a geologic survey of the Fort Peck Indian Reservation. Beekly explored a strip of land along the Montana-North Dakota line from Missouri River to the international boundary, and Bauer examined the townships in which Plentywood and Scobey are situated. Their results are here included with those of the writer, who during the field seasons of 1915 and 1916 was engaged in an investigation of the lignite resources of the remainder of this region, which extends from a line within 12 miles of the Montana-North Dakota boundary westward about 200 miles.

  16. The Montana experience

    NASA Technical Reports Server (NTRS)

    Dundas, T. R.

    1981-01-01

    The development and capabilities of the Montana geodata system are discussed. The system is entirely dependent on the state's central data processing facility which serves all agencies and is therefore restricted to batch mode processing. The computer graphics equipment is briefly described along with its application to state lands and township mapping and the production of water quality interval maps.

  17. Forest resources of Montana

    Treesearch

    S. Blair Hutchinson; Paul D. Kemp

    1952-01-01

    In 1928 Congress passed the McSweeney-McNary Forest Research Act authorizing a comprehensive survey of the timber supplies in the United States. Responsibility for this survey was assigned to the Forest Service of the Department of Agriculture. The work in Montana has been under the direction of the Northern Rockv Mountain Forest and Range Experiment Station. This is a...

  18. Montana Cooperative Education Handbook.

    ERIC Educational Resources Information Center

    Harris, Ron, Ed.

    This revised handbook was developed to help teachers and administrators in Montana conduct cooperative education programs. The handbook is organized in 13 sections. In narrative style, the first 11 sections cover the following topics: introduction to cooperative education, advisory committees, related instruction, coordination of activities,…

  19. Forest regions of Montana

    Treesearch

    Stephen F. Arno

    1979-01-01

    In this paper, Montana is divided into eight geographic subdivisions called "forest regions," based on distributions of tree and undergrowth species and the relationship of these patterns to climate and topography. The regions serve as a geographic reference for describing patterns of forest vegetation across the State. Data on the distributions of plant...

  20. Frontier Schools in Montana: Challenges and Sustainability Practices. A Research Report

    ERIC Educational Resources Information Center

    Harmon, Hobart L.; Morton, Claudette

    2010-01-01

    This study reveals the challenges confronting small, rural "frontier" schools in Montana and the practices that contribute to their sustainability. A Montana frontier school is defined as a school district with 200 or fewer students and its attendant community in a county with five or fewer people per square mile. The researcher…

  1. Enrollment in Higher Education Institutions in the State of Montana, Fall 1978.

    ERIC Educational Resources Information Center

    Dunham, Paul C.

    Data on fall 1978 enrollment in higher education in Montana are summarized and compared with the previous year. Institutions in the Montana state university system, private colleges, and community colleges are included, as are graduate students. Enrollments in noncredit courses are not included in the tables, but are footnoted. Analyses include:…

  2. Forest succession on four habitat types in western Montana

    Treesearch

    Stephen F. Arno; Dennis G. Simmerman; Robert E. Keane

    1985-01-01

    Presents classifications of successional community types on four major forest habitat types in western Montana. Classifications show the sequences of seral community types developing after stand-replacing wildfire and clearcutting with broadcast burning, mechanical scarification, or no followup treatment. Information is provided for associating vegetational response to...

  3. [Study on HPLC-FPS of Pueraria of different sources].

    PubMed

    He, Chun-nian; Li, Min; Cao, Zhi-gao; Guo, Hong-ying; Wang, Chun-lan; Yu, Shi-chun

    2003-12-01

    To make comparative study on HPLC-FPS of several kinds of Pueraria lobata and P. thomsonii from different sources. Kromasil C18 column was used, with mixture of acetonitrile and water as mobile phase in a gradient mode. The wavelength of measurement was 250 nm. The fingerprints of P. lobata and P. thomsonii were obtained. This method can be used to identify P. lobata and P. thomsonii from different sources conveniently, and it may be practically valuable for the quality control of sample for P. lobata or P. thomsonii and its preparation.

  4. Induction of growth hormone release by Pueraria thunbergiana BENTH.

    PubMed

    Jung, D Y; Ha, H; Kim, C

    2004-02-01

    Puerariae Radix (PR), Puerariae Flos (PF), and Puerariae Surculus (PS) as well as their constituents were tested for induction of rat growth hormone (rGH) release by both rat pituitary cell culture and in vivo experimentation in order to develop them to novel drugs. Through a calibration curve of the rGH released by addition of rat growth hormone-releasing hormone (rGHRH) to rat pituitary cells, the 70 % ethanol extracts of PR and PS increased rGH release by about 1.6 and 1.7 times as high, respectively, as the control group (264.6 +/- 13.6 pM). However, each puerarin type as a representative constituent of PR in Korea Pharmacopeia (KP) and tectorigenin and an important ingredient of PF were twice as effective as in the control group. The acid hydrolysate of Puerariae Surculus (HPS) increased rGH release concentration-dependently, and its EC (50) was approximately 10.4 microg/ml. The T (max) value for rGH after injection of 20 microg/kg of rGHRH was 10 - 30 min, while the C (max) value was increased by approximately 12-fold compared to the control group (198.2 +/- 25.0 pM) and the AUC (0 - 45) was increased to 10 times the level of the control group (10,840.9 +/- 845.5 min. pM). On the other hand, T (max) for the HPS was 60 min, while C (max) was increased approximately to 5.8 fold compared to control (244.1 +/- 36.4 pM). C (max) for puerarin was 1,028.6 +/- 502.7 pM, that is, approximately 5.2 times as high as the control level. However, tectorigenin (20 microg/kg) was of no statistical significance. Therefore, we suggest that the HPS and puerarin act either on GH secretagogue receptors or on GHRH receptor of somatotrophin as possible agonists or an inhibitor on somatostatin receptor to release rGH, respectively.

  5. Effectiveness of Written Materials in a Rehabilitative Program for Female Offenders: A Case Study at the Montana Women's Prison

    ERIC Educational Resources Information Center

    Dillon, Laura; Colling, Kyle

    2010-01-01

    This case study of the Therapeutic Community Program at Montana Women's Prison investigates the relationship between inmate reading levels and the self-help materials used for rehabilitative purposes within prison settings. The Therapeutic Community Handbook, published by the Montana Department of Corrections, is used as the primary method of…

  6. Effectiveness of Written Materials in a Rehabilitative Program for Female Offenders: A Case Study at the Montana Women's Prison

    ERIC Educational Resources Information Center

    Dillon, Laura; Colling, Kyle

    2010-01-01

    This case study of the Therapeutic Community Program at Montana Women's Prison investigates the relationship between inmate reading levels and the self-help materials used for rehabilitative purposes within prison settings. The Therapeutic Community Handbook, published by the Montana Department of Corrections, is used as the primary method of…

  7. Geothermal resources of Montana

    SciTech Connect

    Metesh, J.

    1994-06-01

    The Montana Bureau of Mines and Geology has updated its inventory of low and moderate temperature resources for the state and has assisted the Oregon Institute of Technology - GeoHeat Center and the University of Utah Research Institute in prioritizing and collocating important geothermal resource areas. The database compiled for this assessment contains information on location, flow, water chemistry, and estimated reservoir temperatures for 267 geothermal well and springs in Montana. For this assessment, the minimum temperature for low-temperature resource is defined as 10{degree} C above the mean annual air temperature at the surface. The maximum temperature for a moderate-temperature resource is defined as greater than 50{degree} C. Approximately 12% of the wells and springs in the database have temperatures above 50{degree} C, 17% are between 30{degree} and 50{degree} C, 29% are between 20{degree} and 30{degree}C, and 42% are between 10{degree} and 20{degree} C. Low and moderate temperature wells and springs can be found in nearly all areas of Montana, but most are in the western third of the state. Information sources for the current database include the MBMG Ground Water Information Center, the USGS statewide database, the USGS GEOTHERM database, and new information collected as part of this program. Five areas of Montana were identified for consideration in future investigations of geothermal development. The areas identified are those near Bozeman, Ennis, Butte, Boulder, and Camas Prairie. These areas were chosen based on the potential of the resource and its proximity to population centers.

  8. Indians in Montana. Montana Economic Study-Staff Study.

    ERIC Educational Resources Information Center

    Montana Univ., Missoula. Bureau of Business and Economic Research.

    The three papers in this publication discuss American Indians in Montana. "Indian Poverty in Montana: Findings of the 1960 Census" examines data pertaining to the Indians' economic background. Income data, derived from 25 percent of the population, reports income received in 1959 from: (1) wages, salaries, commissions, and tips; (2)…

  9. Developing a Climate Change Boundary Organization: the Montana Adaptation Exchange

    NASA Astrophysics Data System (ADS)

    Whitlock, C. L.; Brooks, S.; Armstrong, T.; Bryan, B.

    2016-12-01

    Small-population large-area states like Montana are often challenged by a need to offer timely and relevant climate-change information that addresses diverse and widely dispersed stakeholder groups. In Montana, filling the gap between science and various types of decision-makers has motivated development of the first Montana Climate Assessment (MCA1), to be released in 2017 with a focus on climate-change impacts for agricultural, water and forestry sectors. To sustain and build on the MCA1 effort, we are also in the process of creating a Boundary Organization (defined by the National Academy of Sciences) called the Montana Adaptation Exchange (the Exchange); this entity will facilitate the flow of information across the boundaries between science, knowledge and implementation. In Montana, the Exchange brings scientists and practitioners together to seek solutions related to climate-change adaptation and other pressing environmental and social-economic challenges. The Montana Adaptation Exchange (1) is a collaborative partnership of members from the science and practitioner communities under a shared governance and participatory model; (2) presents research that has been vetted by the scientific community at large and represents the current state of knowledge; (3) allows for revision and expansion of assessments like the MCA; (4) communicates relevant, often technical, research and findings to a wide variety of resource managers and other stakeholders; (5) develops and maintains an extensive online database that organizes, regularly updates, and makes research data products readily available; and (6) offers an online portal and expert network of affiliated researchers and climate adaptation specialists to provide effective customer support. Boundary organizations, such as the Montana Adaptation Exchange, offer a scalable path to effectively move from "science to knowledge to action" while also allowing stakeholder needs to help inform research agendas.

  10. Thermal stability of kudzu root (Pueraria Radix) isoflavones as additives to beef patties.

    PubMed

    Kumari, S; Raines, J M; Martin, J M; Rodriguez, J M

    2015-03-01

    Kudzu root, Pueraria radix, extracts are a rich source of isoflavones. This study investigates the thermal stability of Pueraria radix extracts as a natural nutraceutical supplement in beef patties. The extract contained puerarin, diadzin, genistin, ononin, daidzein, glycitein, calycosin, genistein, formononetin and biochanin A; however, puerarin, daidzein and glycitein were the main components. The isoflavones concentrations in the spiked beef patties with kudzu root extracts were unaffected by cooking.

  11. Montana geoenvironmental explorer

    USGS Publications Warehouse

    Lee, Greg K.

    2001-01-01

    This report is the result of a multidisciplinary effort to assess relative potential for acidic, metal-rich drainage in the State of Montana; evaluate alternative GIS-based modeling strategies; and provide the statewide digital spatial data produced and compiled for the project. The CD is usable on various computer systems (Windows 95, 98, NT, and 2000; MacOS 7.1 or later; many versions of UNIX and Linux; and OS/2). This report and maps are in PDF format, and the data have been provided in various GIS formats. Software for viewing the report and data is included.

  12. RATTLESNAKE ROADLESS AREA, MONTANA.

    USGS Publications Warehouse

    Wallace, C.A.; Mayerle, Ronald T.

    1984-01-01

    Geologic, geochemical, and geophysical surveys of the Rattlesnake Roadless Area in Montana identified a small area of substantiated resource potential for a low-grade stratabound copper occurrence along the northwest border of the area. A demonstrated barite (BaSO//4) resource of 45 tons and a demonstrated limestone resource suitable for cement production are present in the southern part of the roadless area. Limestone, silica in quartz veins, and sand and gravel deposits are known in the southern part of the area but similar deposits occur widely outside the study area. There is little promise for the occurrence of energy resources in the Rattlesnake Roadless Area.

  13. Future fuels from Montana

    SciTech Connect

    Buchsbaum, L.

    2006-04-15

    To make America less dependent on foreign oil, Montana Governor Brain Schweitzer pushes for investment in synfuel technology. He advocates coal as the 'new fuel' for cars and believes synfuels from coal can bridge the gap between the petroleum economy of the past and the hydrogen economy of the future. He is pushing for a 'Future Fuels' project to form a public-private partnership to build 20 coal conversion, synfuel manufacturing plants. This could contribute to making the USA energy self-sufficient, more quickly than the FutureGen project, he believes.

  14. American Indian High School Completion in Rural Southeastern Montana.

    ERIC Educational Resources Information Center

    Ward, Carol

    1995-01-01

    Factors related to dropping out were examined among Northern Cheyenne and Crow high school students living in three southeastern Montana communities and attending a Catholic school, a public school, or a tribal school. Place of residence, parental educational attainment, and school experiences were important variables, but their effects varied by…

  15. Floods of May 1981 in west-central Montana

    USGS Publications Warehouse

    Parrett, Charles; Omang, R.J.; Hull, J.A.; Fassler, John W.

    1982-01-01

    Extensive flooding occurred in west-central Montana during May 22-23, 1981, as a result of a series of rainstorms. Flooding was particularly severe in the communities of East Helena, Belt, and Deer Lodge. Although no lives were lost, total flood damages were estimated by the Montana Disaster Emergency Services Division to be in excess of $30 million. Peak discharges were determined at 75 sites in the flooded area. At 25 sites the May 1981 peak discharge exceeded the computed 100-year frequency flood, and at 29 sites, where previous flow records are available, the May 1981 peak discharge exceeded the previous peak of record. (USGS)

  16. Estrogenic activity of the dichloromethane extract from Pueraria mirifica.

    PubMed

    Sookvanichsilp, N; Soonthornchareonnon, N; Boonleang, C

    2008-12-01

    Pueraria mirifica and its extracts are widely used as the ingredient(s) in many rejuvenating products. Up to now, the extract of P. mirifica roots that has been used in most studies, is the alcoholic extract. In the present study, we investigated the estrogenic activity using uterotropic and MCF-7 cell proliferation models of the dichloromethane extract as well as the water extract which was obtained from partitioning the ethanolic extract. The results indicated that among the three extracts, i.e. the ethanolic extract (PM1), the water extract (PM2) and dichloromethane extract (PM3), PM3 exhibited the most potent estrogenic activity in both models, followed by PM1. The extracts produced uterotropic activity associated with the increase of water content while uterotropic activity of 17beta-estradiol was related to the increase of muscle mass. The two isoflavonoids, genistein and daidzein, were not the major active phytoestrogens involving the estrogenic activity of these extracts.

  17. Hepatotoxicity of tubers of Indian Kudzu (Pueraria tuberosa) in rats.

    PubMed

    Santosh, Nagwani; Mohan, Kumar; Royana, Singh; Yamini, Tripathi B

    2010-04-01

    Methanolic extract of tubers of Pueraria tuberosa Linn. (Fabaceae) (PTME) has been tested for hepatoxicity in rats. In acute study, PTME (100-400 mg/100 g BW, given orally) showed LD(50) at 227.5 mg. For sub-chronic study, its repeated doses (5-100 mg/100 g BW, for 30 days), significantly increased hepatic enzymes in blood, sinusoidal congestion, disruption of central vein, inflammatory cell infiltration and hepatocellular necrosis in liver in dose dependent manner, with increase in NO, iNOS and ROS levels. In a kinetic study (single dose 227.5 mg/100 g BW), there was sequential decrease in GSH and enhanced NO suggesting free-radical generation as the primary cause of cell damage. It is concluded that the higher dosing of PTME or its continuous use for longer period (even in low doses) is hepatotoxicity by inducing oxidative stress.

  18. Proteome analysis of Pueraria mirifica tubers collected in different seasons.

    PubMed

    Jungsukcharoen, Jutarmas; Chokchaichamnankit, Daranee; Srisomsap, Chantragan; Cherdshewasart, Wichai; Sangvanich, Polkit

    2016-06-01

    Pueraria mirifica-derived tuberous powder has been long-term consumed in Thailand as female hormone-replacement traditional remedies. The protein profiles of tubers collected in different seasons were evaluated. Phenol extraction, 2D-PAGE, and mass spectrometry were employed for tuberous proteome analysis. Out of the 322 proteins detected, over 59% were functionally classified as being involved in metabolism. The rest proteins were involved in defense, protein synthesis, cell structure, transportation, stress, storage, and also unidentified function. The proteins were found to be differentially expressed with respect to harvest season. Importantly, chalcone isomerase, isoflavone synthase, cytochrome p450, UDP-glycosyltransferase, and isoflavone reductase, which are all involved in the biosynthesis pathway of bioactive isoflavonoids, were most abundantly expressed in the summer-collected tubers. This is the first report on the proteomic patterns in P. mirifica tubers in relevant with seasonal variation. The study enlights the understanding of variance isoflavonoid production in P. mirifica tubers.

  19. Isolation of radiolabeled isoflavones from kudzu (Pueraria lobata) root cultures.

    PubMed

    Reppert, Adam; Yousef, Gad G; Rogers, Randy B; Lila, Mary Ann

    2008-09-10

    Isoflavones have potential for preventing and treating several chronic health conditions, such as osteoporosis, cardiovascular disease, and cancer. In this study, radiolabeled isoflavones were recovered from kudzu (Pueraria lobata) root cultures after incubation with uniformly labeled (14)C-sucrose in the culture medium for 21 days. Approximately 19% of administered label was recovered in the isoflavone-rich dried extracts of kudzu root cultures (90.2 microCi/g or 3.3 MBq/g extract). HPLC-PDA analysis revealed the predominant isoflavones isolated from kudzu root cultures to be puerarin, daidzin, and malonyl-daidzin. The average concentration of the major isoflavone puerarin in kudzu root cultures was 33.6 mg/g extract, with a specific activity of 63.5 microCi/g (2.3 MBq/g). The isolated isoflavones were sufficiently (14)C-labeled to permit utilization for subsequent in vivo metabolic tracking studies.

  20. Isolation of Radiolabeled Isoflavones from Kudzu (Pueraria lobata) Root Cultures

    PubMed Central

    Reppert, Adam; Yousef, Gad G.; Rogers, Randy B.; Lila, Mary Ann

    2009-01-01

    Isoflavones have potential for preventing and treating several chronic health conditions, such as osteoporosis, cardiovascular disease, and cancer. In this study, radiolabeled isoflavones were recovered from kudzu (Pueraria lobata) root cultures after incubation with uniformly labeled 14C-sucrose in the culture medium for 21 days. Approximately 19% of administered label was recovered in the isoflavone-rich dried extracts of kudzu root cultures (90.2 μCi/g or 3.3 MBq/g extract). HPLC-PDA analysis revealed the predominant isoflavones isolated from kudzu root cultures to be puerarin, daidzin, and malonyl-daidzin. The average concentration of the major isoflavone puerarin in kudzu root cultures was 33.6 mg/g extract, with a specific activity of 63.5 μCi/g (2.3 MBq/g). The isolated isoflavones were sufficiently 14C-labeled to permit utilization for subsequent in vivo metabolic tracking studies PMID:18690681

  1. Assessment of ecological conditions and potential effects of water produced from coalbed natural gas development on biological communities in streams of the Powder River structural basin, Wyoming and Montana, 2005-08

    USGS Publications Warehouse

    Peterson, David A.; Clark, Melanie L.; Foster, Katharine; Wright, Peter R.; Boughton, Gregory K.

    2010-01-01

    Ongoing development of coalbed natural gas in the Powder River structural basin in Wyoming and Montana led to formation of an interagency task group to address concerns about the effects of the resulting production water on biological communities in streams of the area. The interagency task group developed a monitoring plan and conducted sampling of macroinvertebrate, algal, and fish communities at 47 sites during 2005-08 to document current ecological conditions and determine existing and potential effects of water produced from coalbed natural gas development on biological communities. Macroinvertebrate, algal, and fish community composition varied between drainage basins, among sites within drainage basins, and by year. Macroinvertebrate communities of the main-stem Tongue River were characterized by higher taxa richness and higher abundance of Ephemeroptera, for example, compared to macroinvertebrate communities in plains tributaries of the Tongue River and the main-stem Powder River. Fish communities of the Tongue River were characterized by higher taxa richness and abundance of introduced species compared to the Powder River where native species were dominant. Macroinvertebrate community metric values from sites in the middle reach of the main-stem Powder River, from below Willow Creek to below Crazy Woman Creek, differed from metric values in the upper and lower reaches of the Powder River. Metrics indicative of communitywide differences included measures of taxa richness, relative abundance, feeding mode, and tolerance. Some of the variation in the macroinvertebrate communities could be explained by variation in environmental variables, including physical (turbidity, embeddedness, bed substrate size, and streamflow) and chemical (alkalinity and specific conductance) variables. Of these environmental variables, alkalinity was the best indicator of coalbed natural gas development because of the sodiumbicarbonate signature of the production water. Algal

  2. MADISON ROADLESS AREA, MONTANA.

    USGS Publications Warehouse

    Simons, Frank S.; Lambeth, Robert H.

    1984-01-01

    A mineral-resource survey of the Madison Roadless Area in the Madison Range of southwestern Montana was made. The Madison Roadless Area has demonstrated resources of about 93,000 tons of sillimanite rock at the Placer Creek deposit and of about 83,000 tons of asbestos rock at the Karst deposit. The roadless area also has areas of substantiated phosphate resource potential; much of the phosphate is in thin deeply buried beds. An area near the south edge of the roadless area has a probable resource potential for copper and silver. The concentration of uranium-rich stream-sediment samples in the southwest part of the roadless area suggests that a further attempt to identify the source rocks might be justified.

  3. MONTANA PALLADIUM RESEARCH INITIATIVE

    SciTech Connect

    Peters, John; McCloskey, Jay; Douglas, Trevor; Young, Mark; Snyder, Stuart; Gurney, Brian

    2012-05-09

    Project Objective: The overarching objective of the Montana Palladium Research Initiative is to perform scientific research on the properties and uses of palladium in the context of the U.S. Department of Energy's Hydrogen, Fuel Cells and Infrastructure Technologies Program. The purpose of the research will be to explore possible palladium as an alternative to platinum in hydrogen-economy applications. To achieve this objective, the Initiatives activities will focus on several cutting-edge research approaches across a range of disciplines, including metallurgy, biomimetics, instrumentation development, and systems analysis. Background: Platinum-group elements (PGEs) play significant roles in processing hydrogen, an element that shows high potential to address this need in the U.S. and the world for inexpensive, reliable, clean energy. Platinum, however, is a very expensive component of current and planned systems, so less-expensive alternatives that have similar physical properties are being sought. To this end, several tasks have been defined under the rubric of the Montana Palladium Research Iniative. This broad swath of activities will allow progress on several fronts. The membrane-related activities of Task 1 employs state-of-the-art and leading-edge technologies to develop new, ceramic-substrate metallic membranes for the production of high-purity hydrogen, and develop techniques for the production of thin, defect-free platinum group element catalytic membranes for energy production and pollution control. The biomimetic work in Task 2 explores the use of substrate-attached hydrogen-producing enzymes and the encapsulation of palladium in virion-based protein coats to determine their utility for distributed hydrogen production. Task 3 work involves developing laser-induced breakdown spectroscopy (LIBS) as a real-time, in situ diagnostic technique to characterize PGEs nanoparticles for process monitoring and control. The systems engineering work in task 4 will

  4. [Identification of three-step IR spectra of radix Puerariae from different habitats].

    PubMed

    Han, Ming-Xia; Zhou, Qun; Li, Quan-Hong; Sun, Su-Qin

    2009-07-01

    The IR spectra method and 2D-IR correlation spectra method were used for the identification of radix puerariae from Baxian mountain in Tianjin, Huping mountain in Hunan and Jinfo mountain in Chongqing. The results show that the IR spectra (the first identification) and the second derivative spectra (the second identification) of kudzu roots from different habitats have some similarity. Compared with the spectra of amylum and puerarin, three samples are all rich in amylum, and have some puerarin. The primary difference is the 62 wave numbers between the strongest peak of Tianjin kudzu at 1 048 cm(-1) and amylum at 986 cm(-1), showing that the content of amylum is lower. Radix puerariae from Tianjin has characteristic peaks consistent with those of puerarin at 891, 835 and 797 cm(-1), which means that radix puerariae from Tianjin has more puerarin. In the 2D-IR correlation spectra (the third identification), the three kinds of radix puerariae have the difference in position and the intensity of the correlation peak cluster. Therefore, the three-step IR macro-fingerprint provides a rapid and effective method for the identification of radix puerariae from the different habitats.

  5. Evaluation of the estrogenic activity of the wild Pueraria mirifica by vaginal cornification assay.

    PubMed

    Cherdshewasart, Wichai; Kitsamai, Yosaporn; Malaivijitnond, Suchinda

    2007-04-01

    The aim of this study was to evaluate the estrogenic activity of tuberous samples of phytoestrogen-rich Pueraria mirifica collected from 25 of 76 provinces in Thailand by vaginal cornification assay. Tuberous powders were prepared and administered to ovariectomized rats for 14 consecutive days at dosages of 10, 100 and 1,000 mg/kg BW respectively, and were compared with a daily treatment with 2 mg/kg BW 17beta-estradiol (E(2)). Rats treated with 10 mg/kg BW Pueraria mirifica showed no vaginal cornification. Treatment with 100 mg/kg BW Pueraria mirifica from 13 out of 25 plant samples resulted in development of vaginal cornification. The cell count percentages of the vaginal smeared cells for the treatment with the 2 plant samples that exhibited the fastest vaginal cornification revealed large variation in their estrogenic activities. Treatment with 1,000 mg/kg BW Pueraria mirifica from all plant samples produced vaginal cornification with the mean value for the period (day) of first appearance of cornified cells being 4.08 days compared to 2 days with 2 mg/kg BW E(2). The overall appearance period (day) of cornified cells during the treatment and post-treatment period with 1,000 mg/kg BW per day Pueraria mirifica was shorter than treatment with 2 mg/kg BW E(2). The results demonstrate that the plant population shows differential estrogenic activity as evaluated by vaginal cornification assay.

  6. Metabolism of Isoflavones Found in the Pueraria thomsonii Flower by Human Intestinal Microbiota.

    PubMed

    Hirayama, Kazuhiro; Matsuzuka, Yuki; Kamiya, Tomoyasu; Ikeguchi, Motoya; Takagaki, Kinya; Itoh, Kikuji

    2011-01-01

    Isoflavones contained in the root and flower of Kudzu (Pueraria lobata and related species) are suggested to be the critical component for its effects. Although metabolism of soy isoflavones has been well studied, the composition of isoflavones found in Kudzu is completely different from that of soy isoflavones. In the present study, we investigated whether isoflavones found in the flower of Pueraria thomsonii, a species of Kudzu, were metabolized by human fecal microbiota and murine small intestinal enzymes. Among 5 glycosidic isoflavones of the Pueraria thomsonii flower, tectorigenin 7-O-xylosylglucoside, tectoridin, genistin and glycitin were completely hydrolyzed by a homogenate of germfree mouse small intestine without contribution of bacteria. Released aglycones were not further metabolized, except that up to half of glycitein disappeared. Mouse small intestinal enzymes did not metabolize 6-hydroxygenistein 6,7-di-O-glucoside. Isoflavone aglycones as well as 6-hydroxygenistein 6,7-di-O-glucoside were highly metabolized by most of the human fecal suspensions. Metabolites were not detected with the present analytical methods in most cases. Although further investigations of the pharmacokinetics of Pueraria thomsonii flower isoflavones are needed, the results of the present study indicate active metabolism of Pueraria thomsonii flower isoflavones in the human intestine.

  7. Libraries in Montana: MedlinePlus

    MedlinePlus

    ... this page: https://medlineplus.gov/libraries/montana.html Libraries in Montana To use the sharing features on ... page, please enable JavaScript. Billings Billings Clinic Medical Library 2825 8th Avenue North Billings, MT 59107-5100 ...

  8. Effects of agaricus lilaceps fairy rings on soil aggregation and microbial community structure in relation to growth stimulation of western wheatgrass (pascopyrum smithii) in Eastern Montana rangeland

    USDA-ARS?s Scientific Manuscript database

    Stimulation of plant productivity caused by Agaricus fairy rings has been reported, but nothing is known about soil aggregation and the microbial community structure of the stimulated zone, particularly the communities that can bind to soil particles. We studied three concentric zones of Agaricus li...

  9. 68. Montana Theater, built on North Montana Street in 1901 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    68. Montana Theater, built on North Montana Street in 1901 as Sutton's New Grand Theatre, had a seating capacity of 2,175. In its heyday, this theater attracted the most famous acts of vaudville, opera, and theater. The upper stories are built of yellow painted brick, with terra-cotta applied in a stone pattern on the ground floor. The entrance has been modernized with a wall of glass bricks. On the north side of the theater is a four story building which provided a side entrance to the theater and had storefronts, with apartments above. - Butte Historic District, Bounded by Copper, Arizona, Mercury & Continental Streets, Butte, Silver Bow County, MT

  10. [Determination of puerarin, daidzin and daidzein in root of Pueraria lobata of different origin by HPLC].

    PubMed

    Jin, Wen-shan; Tan, Yu-yuan; Chen, You-gen; Wang, Yan

    2003-01-01

    To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata. The separation was performed in a SYMMETRY C18 column with a mobile phase of methanol-1% HAC solution (gradient elution), the The detection wavelength being 250 nm. The average recovery was respectively 101.7%, 100.7% and 101.7% (n = 3), RSD 0.43%, 0.82% and 1.50% (n = 3) for Puerarin, Daidzin and Daidzein. This method is suitable for the determination of Puerarin, Daidzin and Daidzein in Pueraria lobata and its preparation.

  11. Transcriptomic landscape of Pueraria lobata demonstrates potential for phytochemical study

    PubMed Central

    Han, Rongchun; Takahashi, Hiroki; Nakamura, Michimi; Yoshimoto, Naoko; Suzuki, Hideyuki; Shibata, Daisuke; Yamazaki, Mami; Saito, Kazuki

    2015-01-01

    Pueraria lobata (Willd.) Ohwi has a long and broad application in the treatment of disease. However, in the US and EU, it is treated as a notorious weed. The information to be gained from decoding the deep transcriptome profile would facilitate further research on P. lobata. In this study, more than 93 million fastq format reads were generated by Illumina’s next-generation sequencing approach using five types of P. lobata tissue, followed by CLC de novo assembly methods, ultimately yielding about 83,041 contigs in total. Then BLASTx similarity searches against the NCBI NR database and UniProtKB database were conducted. Once the duplicates among BLASTx hits were eliminated, ID mapping against the UniProt database was conducted online to retrieve Gene Ontology information. In search of the putative genes relevant to essential biosynthesis pathways, all 1,348 unique enzyme commission numbers were used to map pathways against the Kyoto Encyclopedia of Genes and Genomes. Enzymes related to the isoflavonoid and flavonoid biosynthesis pathways were focused for detailed investigation and subsequently, quantitative real-time reverse transcription polymerase chain reaction was conducted for biological validation. Metabolites of interest, puerarin and daidzin were studied by HPLC. The findings in this report may serve as a footstone for further research into this promising medicinal plant. PMID:26157443

  12. Batch foam fractionation of kudzu (Pueraria lobata) vine retting solution.

    PubMed

    Eiamwat, J; Loha, V; Prokop, A; Tanner, R D

    1998-01-01

    The aqueous protein solution from kudzu (Pueraria lobata) vine retting broth, without the addition of other surfactants, was foam-fractionated in a vertical tubular column with multiple sampling ports. Time-varying trajectories of the total protein levels were determined to describe the protein behavior at six positions along the 1-m column. The lowest two trajectories of this batch process represented a loss of proteins from the bulk liquid and tended to merge and decay together in time; the other trajectories displayed a gain in proteins in the foam phase. These upper column port protein concentration trajectories generally increased in time up to 45 mm, followed by a decrease, reflecting the removal of proteins from the column ports. The foam became dryer as it passed up the column to the top port. The protein concentration was about 5-8x higher in the top port foam than in the initial bulk solution, mainly as a result of liquid drainage from the foam along the column axis. This concentration increase in the collected foam was dependent on the initial pH of the bulk solution. The mol-wt profile of the proteins in the concentrated foam effluent was determined by one-dimensional gel electrophoresis. An analysis of the gel electropherograms indicated that the most abundant proteins could be cellulases and pectinases.

  13. An Alternative Pathway for Formononetin Biosynthesis in Pueraria lobata

    PubMed Central

    Li, Jia; Li, Changfu; Gou, Junbo; Wang, Xin; Fan, Rongyan; Zhang, Yansheng

    2016-01-01

    The O-methylation is an important tailing process in Pueraria lobata isoflavone metabolism, but the molecular mechanism governing it remains not elucidated. This manuscript describes the mining of key O-methyltransferases (OMTs) involved in the process. Using our previously constructed P. lobata transcriptome, the OMT candidates were searched, extensively analyzed, and their functions were investigated by expression in yeast, Escherichia coli, or Glycine max hairy roots. Here, we report the identification of the key OMT gene responsible for formononetin production in P. lobata (designated as PlOMT9). PlOMT9 primarily functions as an isoflavone-specific 4′-O-methyltransferase, although it shows high sequence identities with isoflavone 7-O-methyltransferases. Moreover, unlike the previously reported OMTs that catalyze the 4′-O-methylation for formononetin biosynthesis at the isoflavanone stage, PlOMT9 performs this modifying step at the isoflavone level, using daidzein rather than 2,7,4′-trihydroxy-isoflavanone as the substrate. Gene expression analyses and metabolite profiling supported its proposed roles in P. lobata. Using the system of transgenic G. max hairy roots, the role of PlOMT9 in the biosynthesis of formononetin was further demonstrated in vivo. PMID:27379141

  14. Medical applications of phytoestrogens from the Thai herb Pueraria mirifica.

    PubMed

    Malaivijitnond, Suchinda

    2012-03-01

    Pueraria mirifica Airy Shaw et Suvatabandhu is a medicinal plant endemic to Thailand. It has been used in Thai folklore medicine for its rejuvenating qualities in aged women and men for nearly one hundred years. Indeed, it has been claimed that P. mirifica contains active phytoestrogens (plant substances with estrogen-like activity). Using high performance liquid chromatography, at least 17 phytoestrogens, mainly isoflavones, have been isolated. Thus, fairly considerable scientific researches, both in vitro in cell lines and in vivo in various species of animals including humans, have been conducted to date to address its estrogenic activity on the reproductive organs, bones, cardiovascular diseases and other climacteric related symptoms. The antioxidative capacity and antiproliferative effect on tumor cell lines have also been assessed. In general, P. mirifica could be applicable for preventing, or as a therapeutic for, the symptoms related to estrogen deficiency in menopausal women as well as in andropausal men. However, the optimal doses for each desirable effect and the balance to avoid undesired side effects need to be calculated before use.

  15. Requirement of metabolic activation for estrogenic activity of Pueraria mirifica.

    PubMed

    Lee, Y S; Park, J S; Cho, S D; Son, J K; Cherdshewasart, W; Kang, K S

    2002-12-01

    A wide range of chemicals derived from plant and human-made xenobiotics are reported to have hormonal activities. The present study was performed to examine the estrogenic effect of Kwao Keur, Pueraria mirifica (PM), that has been used as a rejuvenating folk medicine in Thailand, using recombinant yeast, MCF-7 cell proliferation and HepG2 cell transient transfection assay. In recombinant yeast assay, 0.025, 0.25, 2.5, 25, 2.5 x 10(2), 2.5 x 10(3), 2.5 x 10(4) ng/ml concentrations of PM did not show any estrogenic activities, while 10(-9) of 17 beta-estradiol (positive control) showed high estrogenic activity. Estrogenic activities were induced at 2.5 ng/ml to 25 microg/ml concentrations of PM in a dose-dependent manner on MCF-7 cells and the estrogenic effect of PM was blocked by tamoxifen treatment, a well-known anti-estrogen. PM also showed estrogenic effect on human hepatoma cell line, HepG2 cells, containing estrogen receptor and luciferase reporter gene. Taken together, PM in itself may have no estrogenicity in yeast system, but it has estrogenicity in MCF-7 & HepG2 cells that have human metabolic enzymes. The results indicated that PM may require metabolic activation for estrogenic activity.

  16. Pueraria tuberosa: a review on its phytochemical and therapeutic potential.

    PubMed

    Maji, Amal K; Pandit, Subrata; Banerji, Pratim; Banerjee, Debdulal

    2014-01-01

    Pueraria tuberosa (Willd.) DC is a perennial herb commonly known as 'vidarikanda', distributed throughout south east Asia. The plant's tuber is widely used in ethanomedicine as well as in traditional systems of medicine, particularly in ayurveda. It has been used in various ayurvedic formulations as restorative tonic, antiaging, spermatogenic and immune booster and has been recommended for the treatment of cardiovascular diseases, hepatosplenomegaly, fertility disorders, menopausal syndrome, sexual debility and spermatorrhoea. Numerous bioactive phytochemicals, mostly isoflavonoids such as puerarin, genistein, daidzein, tuberosin and so on have been identified in the tuber. In vivo and in vitro studies have provided the support against traditional demands of the tuber as spermatogenic, immune booster, aphrodisiac, anti-inflammatory, cardiotonic and brain tonic. However, further studies are required to define the active phytochemical compositions and to validate its clinical utilisation in the herbal formulations for human uses. This review provides an overview of traditional applications, current knowledge on the phytochemistry, pharmacology and toxicology of P. tuberosa. This review also provides plausible hypotheses about how various isoflavones particularly puerarin, genistein and daidzein, individually or collectively, may be responsible for the therapeutic potential against a wide range of ailments.

  17. Pueraria mirifica leaves, an alternative potential isoflavonoid source.

    PubMed

    Jungsukcharoen, Jutarmas; Dhiani, Binar Asrining; Cherdshewasart, Wichai; Vinayavekhin, Nawaporn; Sangvanich, Polkit; Boonchird, Chuenchit

    2014-01-01

    We investigated the major leaf isoflavonoid contents of Pueraria mirifica from three different cultivars (PM-III, PM-IV, and PM-V) using reverse RP-HPLC analysis. The proportions and net levels of puerarin, daidzin, genistin, and daidzein in P. mirifica leaves were found to depend on the plant cultivar and to correlate with cultivation temperature and rainfall amount. The crude leaf-extracts were tested using the Yeast Estrogen Screen (YES) assay with both human estrogen receptors (hERα and hERβ). Their estrogenic activity was higher when determined by the YES system containing hERβ than that with hERα and was also higher when the Δsnq2 than the wildtype yeast was employed. The results open the possibility of selecting and cultivating certain P. mirifica cultivars at a farm scale to produce a sufficient supply of leaf material to act as a starting source for the commercial scale extraction of these major isoflavonoids.

  18. A genomic approach to isoflavone biosynthesis in kudzu (Pueraria lobata).

    PubMed

    He, XianZhi; Blount, Jack W; Ge, Shujun; Tang, Yuhong; Dixon, Richard A

    2011-04-01

    Roots of kudzu (Pueraria lobata) are a rich source of isoflavone O- and C-glycosides. Although O-glycosylation of (iso)flavonoids has been well characterized at the molecular level, no plant isoflavonoid C-glycosyltransferase genes have yet been isolated. To address the biosynthesis of kudzu isoflavonoids, we generated 6,365 high-quality expressed sequence tags (ESTs) from a subtraction cDNA library constructed using RNA from roots that differentially accumulate puerarin. The ESTs were clustered into 722 TCs and 3,913 singletons, from which 15 family I glycosyltransferases (UGTs) were identified. Hierarchical clustering analysis of the expression patterns of these UGTs with isoflavone synthase (IFS) in a range of tissues identified UGTs with potential functions in isoflavone glycosylation. The open reading frames of these UGTs were expressed in E. coli for functional analysis, and one was shown to preferentially glycosylate isoflavones at the 7-O-position. In addition, ESTs corresponding to chalcone synthase, chalcone reductase, chalcone isomerase (CHI) and 2-hydroxyisoflavanone dehydratase were identified. Recombinant CHI proteins had high activities with both 6'-deoxy- and 6'-hydroxy chalcones, typical of Type II CHIs. Establishment of this EST database and identification of genes associated with kudzu isoflavone biosynthesis and glycosylation provide a new resource for metabolic engineering of bioactive kudzu isoflavones.

  19. Transcriptomic landscape of Pueraria lobata demonstrates potential for phytochemical study.

    PubMed

    Han, Rongchun; Takahashi, Hiroki; Nakamura, Michimi; Yoshimoto, Naoko; Suzuki, Hideyuki; Shibata, Daisuke; Yamazaki, Mami; Saito, Kazuki

    2015-01-01

    Pueraria lobata (Willd.) Ohwi has a long and broad application in the treatment of disease. However, in the US and EU, it is treated as a notorious weed. The information to be gained from decoding the deep transcriptome profile would facilitate further research on P. lobata. In this study, more than 93 million fastq format reads were generated by Illumina's next-generation sequencing approach using five types of P. lobata tissue, followed by CLC de novo assembly methods, ultimately yielding about 83,041 contigs in total. Then BLASTx similarity searches against the NCBI NR database and UniProtKB database were conducted. Once the duplicates among BLASTx hits were eliminated, ID mapping against the UniProt database was conducted online to retrieve Gene Ontology information. In search of the putative genes relevant to essential biosynthesis pathways, all 1,348 unique enzyme commission numbers were used to map pathways against the Kyoto Encyclopedia of Genes and Genomes. Enzymes related to the isoflavonoid and flavonoid biosynthesis pathways were focused for detailed investigation and subsequently, quantitative real-time reverse transcription polymerase chain reaction was conducted for biological validation. Metabolites of interest, puerarin and daidzin were studied by HPLC. The findings in this report may serve as a footstone for further research into this promising medicinal plant.

  20. Use of benthic invertebrate community structure and the sediment quality triad to evaluate metal-contaminated sediment in the upper Clark Fork River, Montana

    USGS Publications Warehouse

    Canfield, Timothy J.; Kemble, Nile E.; Brumbaugh, William G.; Dwyer, F. James; Ingersoll, Christopher G.; Fairchild, James F.

    1994-01-01

    The upper Clark Fork River, above Flathead River, is contaminated with large amounts of As, Cd, Cu, Pb, Mn, and Zn ores from past mining activities. The contaminated area extends from the Butte and Anaconda area to at least 230 km downstream to Milltown Reservoir. Both the upper Clark Fork River and Milltown Reservoir have been designated as U.S. Environmental Protection Agency Superfund sites because of metal-contaminated bottom sediments. We evaluated the impacts of past mining activities on the Clark Fork River ecosystem using benthic invertebrate community assessment, residue chemistry, and toxicity testing. Oligochaeta and Chironomidae generally accounted for over 90% of the benthic invertebrate community in the soft sediment depositional areas. Taxa of Oligochaeta and Chironomidae were predominantly pollution tolerant. Higher numbers of Chironomidae genera were present at stations with higher concentrations of metals in sediment identified as toxic by the amphipod Hyalella azteca in 28-d exposures. Frequency of mouthpart deformities in genera of Chironomidae was low and did not correspond to concentrations of metals in sediment. Total abundance of organisms/m2 did not correspond to concentrations of metals in the sediment samples. Chemical analyses, laboratory toxicity tests, and benthic community evaluations all provide evidence of metal-induced degradation to aquatic communities in both the reservoir and the river. Using a weight-of-evidence approach-the Sediment Quality Triad - provided good concurrence among measures of benthic community structure, sediment chemistry, and laboratory toxicity.

  1. Use of benthic invertebrate community structure and the sediment quality triad to evaluate metal-contaminated sediment in the upper Clark Fork River, Montana

    SciTech Connect

    Canfield, T.J.; Kemble, N.E.; Brumbaugh, W.G.; Dwyer, F.J.; Ingersoll, C.G.; Fairchild, J.F. . Midwest Science Center)

    1994-12-01

    The upper Clark Fork River, above Flathead River, is contaminated with large amounts of As, Cd, Cu, Pb, Mn, and Zn ores from past mining activities. The contaminated area extends from the Butte and Anaconda area to at least 230 km downstream to Milltown Reservoir. Both the upper Clark Fork River and Milltown Reservoir have been designated as US Environmental Protection Agency Superfund sites because of metal-contaminated bottom sediments. The authors evaluated the impacts of past mining activities on the Clark Fork River ecosystem using benthic invertebrate community assessment, residue chemistry, and toxicity testing. Oligochaeta and Chironomidae generally accounted for over 90% of the benthic invertebrate community in the soft sediment depositional areas. Taxa of Oligochaeta and Chironomidae were predominantly pollution tolerant. Higher numbers of Chironomidae genera were present at stations with higher concentrations of metals in sediment identified as toxic by the amphipod Hyalella azteca in 28-d exposures. Frequency of mouthpart deformities in genera of Chironomidae was low and did not correspond to concentrations of metals in sediment. Total abundance of organisms/m[sup 2] did not correspond to concentrations of metals in the sediment samples. Chemical analyses, laboratory toxicity tests, and benthic community evaluations all provide evidence of metal-induced degradation to aquatic communities in both the reservoir and the river. Using a weight-of-evidence approach--the Sediment Quality Triad--provided good concurrence among measures of benthic community structure, sediment chemistry, and laboratory toxicity.

  2. Macrocyclic trichothecenes are undetectable in kudzu (Pueraria montana) plants treated with a high-producing isolate of Myrothecium verrucaria.

    PubMed

    Abbas, H K; Tak, H; Boyette, C D; Shier, W T; Jarvis, B B

    2001-09-01

    Myrothecium verrucaria was found to be an effective pathogen against kudzu grown in the greenhouse and the field. M. verrucaria produced large amounts of macrocyclic trichothecenes when cultured on solid rice medium, including epiroridin E (16.8 mg/g crude extract), epiisororidin E (1 mg/g), roridin E (8.7 mg/g), roridin H (31.3 mg/g), trichoverrin A (0.6 mg/g), trichoverrin B (0.1 mg/g), verrucarin A (37.4 mg/g), and verrucarin J (2.2 mg/g). Most of these toxins were also isolated from M. verrucaria spores and mycelia grown on potato dextrose agar medium, including epiroridin E (32.3 mg/g), epiisororidin E (28.6 mg/g), roridin E (0 mg/g), roridin H (60 mg/g), trichoverrin A (1.3 mg/g), trichoverrin B (1.8 mg/g), verrucarin A (13.8 mg/g), and verrucarin J (131 mg/g). When M. verrucaria was cultured on liquid media, the numbers but not the amounts of toxins decreased. Only epiroridin E (28.3 mg/g), epiisororidin E (29.6 mg/g), verrucarin B (195 mg/g) and verrucarin J (52.6 mg/g) were measured when the fungus was cultured on cornsteep medium. On soyflour-cornmeal broth M. verrucaria produced several toxins, including epiroridin E (58.1 mg/g), epiisororidin E (5.8 mg/g), verrucarin B (29.9 mg/g) and verrucarin J (32 mg/g). In contrast, no macrocyclic trichothecenes were detected by HPLC analysis of plant tissues of kudzu, sicklepod, and soybean treated with aqueous suspensions of M. verrucaria spores formulated with a surfactant. Chloroform-methanol extracts of kudzu leaves and stems treated with M. verrucaria spores were less cytotoxic to four cultured mammalian cell lines than the corresponding extracts from control plants. Purified macrocyclic trichothecenes (verrucarin A and T-2 toxin) were very cytotoxic to the same cell lines (< or = 2 ng/ml). These results show that neither intact macrocyclic trichothecenes nor toxic metabolites could be detected in plant tissues after treatment with M. verrucaria spores. These results argue for both safety and efficacy for the use of M. verrucaria in biological control of kudzu and other noxious weeds, and support proceeding to animal feeding trials for further evaluation of safety.

  3. Corrections in Montana: A Consultation on Corrections in Montana.

    ERIC Educational Resources Information Center

    Montana State Advisory Committee to the U.S. Commission on Civil Rights, Helena.

    The findings and recommendations of a two-day conference on the civil and human rights of inmates of Montana's correctional institutions are contained in this report. The views of private citizens and experts from local, state, and federal organizations are presented in edited form under seven subject headings: existing prison reform legislation,…

  4. Comparison of Pueraria lobata with hormone replacement therapy in treating the adverse health consequences of menopause.

    PubMed

    Woo, Jean; Lau, Edith; Ho, Suzanne C; Cheng, Francis; Chan, Cynthia; Chan, Agnes S Y; Haines, Christopher J; Chan, Thomas Y K; Li, Martin; Sham, Aprille

    2003-01-01

    Pueraria lobata (PL) is used as a traditional Chinese herbal remedy for menopausal symptoms, as well as an ingredient in preparations for conditions affecting menopausal women, such as osteoporosis, coronary heart disease, and some hormone-dependent cancers. The scientific basis for its action may be its action as a phytoestrogen. To examine the effects of PL in comparison with hormone replacement therapy (HRT) on lipid profile, sex hormone levels, bone turnover markers, and indices of cognitive function. For the study, 127 community-living, postmenopausal women aged 50 to 65 years were randomized to receive HRT (n = 43), PL (equivalent to 100 mg isoflavone; n = 45), or no treatment (n = 39) for 3 months. The following measurements were carried out at baseline and after 3 months for all participants: menopausal symptoms questionnaire; neuropsychological tests covering memory, attention, motor speed, and word-finding ability; quality of life (SF36); lipid profile; urinary deoxypyridinoline; dietary phytoestrogen intake and urinary phytoestrogen; estradiol; follicle-stimulating hormone; and luteinizing hormone. Only participants in the HRT group showed a mean reduction in cholesterol and low-density lipoprotein cholesterol that was significantly different from that of the control group. No significant changes in lipid profile or follicle-stimulating hormone and luteinizing hormone were observed in the PL group compared with the controls. However, both the HRT and PL groups showed an improvement in Mini-Mental State Examination score and attention span compared with the case of participants receiving no treatment. HRT and PL had different effects on cognitive function; HRT improved delayed recall, whereas flexible thinking seemed improved in the PL group. This study was unable to demonstrate a scientific basis for the use of PL for improving the health of postmenopausal women in general. However, the effect of PL on cognitive function deserves further study.

  5. Montana Rural Education Curriculum Guide.

    ERIC Educational Resources Information Center

    Kroon, Ralph, Comp.

    The material in this K-8 curriculum guide is designed to provide classroom professionals in rural Montana schools with some guidance as to when to introduce and develop concepts in each subject area. It is intended to be a guide, not a rule book or complete course of study. For each subject area and for each grade level, topics are coded as I…

  6. Antidote: Civic Responsibility. Montana Law.

    ERIC Educational Resources Information Center

    Phi Alpha Delta Law Fraternity International, Washington, DC.

    Designed for middle school through high school students, this unit contains eight lesson plans that focus on Montana state law. The state lessons correspond to lessons in the volume, "Antidote: Civic Responsibility. Drug Avoidance Lessons for Middle School & High School Students." Developed to be presented by educators, law student,…

  7. Forest habitat types of Montana

    Treesearch

    Robert D. Pfister; Bernard L. Kovalchik; Stephen F. Arno; Richard C. Presby

    1977-01-01

    A land-classification system based upon potential natural vegetation is presented for the forests of Montana. It is based on an intensive 4-year study and reconnaissance sampling of about 1,500 stands. A hierarchical classification of forest sites was developed using the habitat type concept. A total of 9 climax series, 64 habitat types, and 37 additional phases of...

  8. The Montana Agricultural Manpower Project

    ERIC Educational Resources Information Center

    Amberson, Max L.; Bishop, Douglas

    1976-01-01

    The six-year study utilized a series of surveys to identify: educational needs and occupational opportunities related to agriculture (production, processing, mechanics, and service areas) in Montana, competencies needed by workers employed in various job titles, and groups of identified competencies for inclusion in the State high school…

  9. Indian Child Welfare in Montana.

    ERIC Educational Resources Information Center

    Dull Knife Memorial Coll., Lame Deer, MT.

    This report is based upon a 1985-86 survey conducted by the Dull Knife Memorial College Indian Child Welfare Project. A series of workshops were conducted throughout Montana to acquaint providers of services for abused and neglected Indian children with the requirements of and issues associated with the Indian Child Welfare Act of 1978.…

  10. Who Will Teach Montana's Children?

    ERIC Educational Resources Information Center

    Nielson, Dori Burns

    Montana is experiencing three types of teacher shortages, each requiring different intervention strategies. These situations include shortages in specific subject areas, most notably in music, special education, and foreign languages, followed closely by guidance and library; many job openings, caused by rapid enrollment growth, a large number of…

  11. Stability and quality of herb (Pueraria Tuberosa)-milk model system.

    PubMed

    Sawale, Pravin Digambar; Singh, R R B; Arora, Sumit

    2015-02-01

    The medicinal benefits of herbs could be conveyed via certain foods as carriers. Milk is one of the important carrier which has been effectively used to deliver phytochemicals presents in herbs (mainly polyphenols) for targeted health benefits in the traditional Indian system of medical science. The present study was conducted to evaluate the effect of herb components (Pueraria tuberosa) on properties of Pueraria tuberosa-milk model system. The herb was added into cow milk on the basis of sensory evaluation (0.4 %) by using 9-point hedonic scale. The physical and chemical changes were evaluated after various processing treatments viz. pasteurization (72 °C, 15 s), sterilization (121 °C, 15 min), separation etc. These changes were determined using viscosity, hydroxy methyl furfural (HMF) value, ethanol stability, colour characteristics and trolox equivalent antioxidant capacity (TEAC). It was observed that addition of Pueraria tuberosa to milk resulted in decreased HMF content, ethanol stability and lightness whereas antioxidant activity, redness and yellowness increased as compared to control. It can be concluded that addition of Pueraria tuberosa to milk at 0.4 % concentration altered the functional properties of milk and Pueraira tuberosa could be suitable for preparation of low heat treated functional dairy food products.

  12. An evidence-based systematic review of kudzu (Pueraria lobata) by the Natural Standard Research Collaboration.

    PubMed

    Ulbricht, Catherine; Costa, Dawn; Dam, Chi; D'Auria, Denise; Giese, Nicole; Isaac, Richard; LeBlanc, Yvonne; Rusie, Erica; Weissner, Wendy; Windsor, Regina C

    2015-03-01

    An evidence-based systematic review of kudzu (Pueraria lobata) by the Natural Standard Research Collaboration consolidates the safety and efficacy data available in the scientific literature using a validated, reproducible grading rationale. This article includes written and statistical analysis of clinical trials, plus a compilation of expert opinion, folkloric precedent, history, pharmacology, kinetics/dynamics, interactions, adverse effects, toxicology, and dosing.

  13. Extraction technology of isoflavone from Dabie Mountain's Pueraria lobata and determination of its reducing power.

    PubMed

    Renshu, Huang; Huili, He; Jiaojiao, Fang; Yuanjun, Ren

    2014-11-01

    In order to determine the optimum extracting process of the isoflavone from Dabie Mountain's Pueraria lobata and its reducing power, a 5-level, 4-factor Box-Behnken center-united experiment was conducted on the basis of single factor experiment. The Box-Behnken experiment analyzed and optimized the processing conditions by response surface methodology, and determined the reducing power of the Pueraria Isoflavone's extract under the optimum conditions. The results showed that the extraction rate of the Pueraria Isoflavone was 24.87%, of which the relative error was only 0.4% compared with the predicted value of 24.77%, under the optimum conditions of ethanol concentration 7.5%, liquid-to-solid ratio17.50 mL/g, treatment temperature 94.30°C, treatment time 47.36 min. The experiment also concluded that response surface methodology was suitable for the regression analysis of isoflavone's extraction rate and parameter optimization; the reducing power of Pueraria Isoflavone's EC50 value was 0.098 mg/mL.

  14. Pueraria mirifica alleviates cortical bone loss in naturally menopausal monkeys.

    PubMed

    Kittivanichkul, Donlaporn; Charoenphandhu, Narattaphol; Khemawoot, Phisit; Malaivijitnond, Suchinda

    2016-11-01

    Since the in vitro and in vivo anti-osteoporotic effects of Pueraria mirifica (PM) in rodents have been verified, its activity in menopausal monkeys was evaluated as required before it can be applicable for human use. In this study, postmenopausal osteoporotic monkeys were divided into two groups (five per group), and fed daily with standard diet alone (PMP0 group) or diet mixed with 1000 mg/kg body weight (BW) of PM powder (PMP1000 group) for 16 months. Every 2 months, the bone mineral density (BMD), bone mineral content (BMC) and bone geometry parameters (cortical area and thickness and periosteal and endosteal circumference) at the distal radius and proximal tibia were determined using peripheral quantitative computed tomography together with plasma and urinary bone markers. Compared with the baseline (month 0) values, the cortical, but not trabecular, BMDs and BMCs and the cortical area and thickness at the metaphysis and diaphysis of the radius and tibia of the PMP0 group continuously decreased during the 16-month study period. In contrast, PMP1000 treatment ameliorated the bone loss mainly at the cortical diaphysis by decreasing bone turnover, as indicated by the lowered plasma bone-specific alkaline phosphatase and osteocalcin levels. Generally, changes in the cortical bone geometry were in the opposite direction to the cortical bone mass after PMP1000 treatment. This study indicated that postmenopausal monkeys continuously lose their cortical bone compartment, and they have a higher possibility for long bone fractures. Oral PMP treatment could improve both the bone quantity (BMC and BMD) and quality (bone geometry). © 2016 Society for Endocrinology.

  15. Effects of Pueraria mirifica on vascular function of ovariectomized rabbits.

    PubMed

    Wattanapitayakul, Suvara K; Chularojmontri, Linda; Srichirat, Supatra

    2005-06-01

    Estrogen stimulates endothelial nitric oxide (NO) production and attenuates endothelial dysfunction in ischemia/repurfusion and menopause. Recent studies have shown that phytoestrogens from dietary sources improve endothelial function and reduce cardiovascular risks. The Thai medicinal plant Pueraria mirifica (PM) contains many potent phytoestrogens including miroestrol and deoxymiroestrol but no study on vascular function has been established. Ground powder of PM was orally given to ovariectomized White New Zealand rabbits (OVX + PM group) (n = 4) weighing 3.2-4.0 kg at the dose of 100 mg/kg for 90 days. Saline-treated ovariectomized rabbits were assigned as a control group (OVX group) (n = 5). At the end of treatment thoracic aorta was isolated for functional evaluation. Maximal relaxant response to acetylcholine (ACh) was significantly increased (24%) with 3.5-fold decrease in EC50 while no change in relaxant response to sodium nitroprusside was observed Minimal and maximal responses to 17beta-estradiol (E2) were increased in the OVX + PM group and L-NAME (100 mM) attenuated Emax of E2. PM significantly decreased maximal contractile responses to norepinephrine (NE), but no change in EC50 was observed. In addition to vascular study, the authors found no significant alteration in serum cholesterol, LDL, triglyceride, HDL, ALT AST alkaline phosphatase, and lipid peroxidation in OVX + PM rabbits. These data demonstrate that PM (100 mg/kg/d) improved endothelial function through NO-dependent pathway and increased response to E2 while sensitivity to NE was reduced. In addition, it had no impact on lipid profile, liver enzymes, and ALP activities. PM is a potential source of phytoestrogens for postmenopausal women to improve cardiovascular function or reduce cardiovascular risks.

  16. Marigolds for Montana.

    ERIC Educational Resources Information Center

    Woolbaugh, Walt; Dunkel, Florence

    2003-01-01

    Describes a collaborative science research project designed to help build strong school and community relationships. Participating students joined with university scientists on a yearlong agriculturally-based research project to study the effect of marigolds on nematode management. (KHR)

  17. Marigolds for Montana.

    ERIC Educational Resources Information Center

    Woolbaugh, Walt; Dunkel, Florence

    2003-01-01

    Describes a collaborative science research project designed to help build strong school and community relationships. Participating students joined with university scientists on a yearlong agriculturally-based research project to study the effect of marigolds on nematode management. (KHR)

  18. [Traditional Chinese medicine pairs (III)--effect of extract of Ginseng Radix et Rhizoma and Puerariae Lobatae Radix on intestinal absorption in rats].

    PubMed

    Chen, Yi-hang; Li, Meng-xuan; Meng, Zhao-qing; Yang, Jiao-jiao; Huang, Wen-zhe; Wang, Zhen-zhong; Wang, Yue-sheng; Xiao, Wei

    2015-08-01

    This study focused on the intestinal absorption of traditional Chinese medicines (TCM) to reveal the scientific connotation of the compatibility of TCM pairs. The single pass intestinal perfusion (SPIP) was used in rats to compare the absorption of single extracts from Puerariae Lobatae Radix, single extracts from Ginseng Radix et Rhizoma, combined extracts from Puerariae Lobatae Radix and Ginseng Radix et Rhizoma and Puerariae Lobatae Radix and Ginseng Radix et Rhizoma mixture in rats. The content of puerarin, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in liquid were tested by HPLC. The speed constant (Ka) and apparent permeability coefficients (Papp) were calculated and compared. Specifically, the order of puerarin Ka and Papp values from high to low was Ginseng Radix et Rhizoma and Puerariae Lobatae Radix mixture > single extracts from Puerariae Lobatae Radix > combined extracts from Ginseng Radix et Rhizoma and Puerariae Lobatae Radix; the order of ginsenosides Ka and Papp values from high to low was Ginseng Radix et Rhizoma and Puerariae Lobatae Radix mixture > single extracts from Ginseng Radix et Rhizoma > combined extracts from Ginseng Radix et Rhizoma and Puerariae Lobatae Radix. The combined administration of Ginseng Radix et Rhizoma and Puerariae Lobatae Radix may improve the absorption in the intestinal tract.

  19. Smoke over Montana and Wyoming

    NASA Technical Reports Server (NTRS)

    2002-01-01

    California was not the only western state affected by fire during the last weekend of July. Parts of Montana and Wyoming were covered by a thick pall of smoke on July 30, 2000. This true-color image was captured by the Sea-viewing Wide Field-of-view Sensor (SeaWiFS). It is much easier to distinguish smoke from cloud in the color SeaWiFS imagery than the black and white Geostationary Operational Environmental Satellite (GOES) imagery. However, GOES provides almost continuous coverage (animation of Sequoia National Forest fire) and has thermal infrared bands (Extensive Fires in the Western U.S.) which detect the heat from fires. On Monday July 31, 2000, eight fires covering 105,000 acres were burning in Montana, and three fires covering 12,000 acres were burning in Wyoming. Image provided by the SeaWiFS Project, NASA/Goddard Space Flight Center, and ORBIMAGE

  20. Smoke over Montana and Wyoming

    NASA Technical Reports Server (NTRS)

    2002-01-01

    California was not the only western state affected by fire during the last weekend of July. Parts of Montana and Wyoming were covered by a thick pall of smoke on July 30, 2000. This true-color image was captured by the Sea-viewing Wide Field-of-view Sensor (SeaWiFS). It is much easier to distinguish smoke from cloud in the color SeaWiFS imagery than the black and white Geostationary Operational Environmental Satellite (GOES) imagery. However, GOES provides almost continuous coverage (animation of Sequoia National Forest fire) and has thermal infrared bands (Extensive Fires in the Western U.S.) which detect the heat from fires. On Monday July 31, 2000, eight fires covering 105,000 acres were burning in Montana, and three fires covering 12,000 acres were burning in Wyoming. Image provided by the SeaWiFS Project, NASA/Goddard Space Flight Center, and ORBIMAGE

  1. The Marysville, Montana Geothermal Project

    NASA Technical Reports Server (NTRS)

    Mcspadden, W. R.; Stewart, D. H.; Kuwada, J. T.

    1974-01-01

    Drilling the first geothermal well in Montana presented many challenges, not only in securing materials and planning strategies for drilling the wildcat well but also in addressing the environmental, legal, and institutional issues raised by the request for permission to explore a resource which lacked legal definition. The Marysville Geothermal Project was to investigate a dry hot rock heat anomaly. The well was drilled to a total depth of 6790 feet and many fractured water bearing zones were encountered below 1800 feet.

  2. ANACONDA-PINTLAR WILDERNESS, MONTANA.

    USGS Publications Warehouse

    Elliott, J.E.; Close, T.J.

    1984-01-01

    A mineral survey of the Anaconda-Pintlar Wilderness, an area of about 250 sq mi in southwestern Montana, was conducted. Results of this survey indicate that parts of the area have probable and (or) substantiated resource potential for silver, copper, molybdenum, lead, tungsten, tin, gold, and zinc. Based on the nature of the geologic terrain, there is little likelihood of the occurrence of geothermal, coal, oil, or gas resources.

  3. SPANISH PEAKS PRIMITIVE AREA, MONTANA.

    USGS Publications Warehouse

    Calkins, James A.; Pattee, Eldon C.

    1984-01-01

    A mineral survey of the Spanish Peaks Primitive Area, Montana, disclosed a small low-grade deposit of demonstrated chromite and asbestos resources. The chances for discovery of additional chrome resources are uncertain and the area has little promise for the occurrence of other mineral or energy resources. A reevaluation, sampling at depth, and testing for possible extensions of the Table Mountain asbestos and chromium deposit should be undertaken in the light of recent interpretations regarding its geologic setting.

  4. DOLUS LAKES ROADLESS AREA, MONTANA.

    USGS Publications Warehouse

    Elliott, James E.; Avery, Dale W.

    1984-01-01

    A mineral survey of the Dolus Lakes Roadless Area in southwestern Montana, was conducted. Much of the roadless area has probable and substantiated potential for resources of gold, silver, molybdenum, and tungsten. The nature of the geologic terrain indicates that there is little promise for the occurrence of coal, oil, gas, or geothermal resources. Detailed geologic and geochemical studies are suggested to delineate exploration targets that could be tested by drilling.

  5. 76 FR 71355 - United States et al. v. Blue Cross and Blue Shield of Montana, Inc. et al.; Proposed Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-17

    ... hospital defendants are Billings Clinic, Bozeman Deaconess Health Services, Inc., Community Medical Center... Deaconess Health Services, Inc., Community Medical Center, Inc., New West Health Services, Inc., Northern... Services, Inc.; Community Medical Center, Inc.; Northern Montana Health Care, Inc.; and St. Peter's...

  6. 76 FR 12857 - Montana Regulatory Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-09

    ... Office of Surface Mining Reclamation and Enforcement 30 CFR Part 926 Montana Regulatory Program AGENCY: Office of Surface Mining Reclamation and Enforcement, Interior. ACTION: Final rule; approval of amendment... the Surface Mining Control and Reclamation Act of 1977 (``SMCRA'' or ``the Act''). Montana...

  7. 77 FR 48198 - Montana Disaster #MT-00068

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-13

    ... ADMINISTRATION Montana Disaster MT-00068 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Montana dated 08/06/2012. Incident: Dahl Fire. Incident Period: 06/26/2012 through 07/06/2012. Effective Date: 08/06/2012...

  8. 77 FR 47907 - Montana Disaster #MT-00067

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-10

    ... ADMINISTRATION Montana Disaster MT-00067 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of MONTANA dated 08/02/2012. Incident: Ash Creek Fire. Incident Period: 06/25/2012 through 07/22/2012. Effective Date: 08/02...

  9. An Environmental Education Plan for Montana.

    ERIC Educational Resources Information Center

    Jackson, John Y.

    The purpose of this dissertation was to develop an environmental education master plan for implementing environmental education programs into the schools of Montana. The process used to develop the plan consisted of the following steps: (1) to examine environmental education plans from other states; (2) to determine what Montana educators felt…

  10. Helminth parasites of spotted sandpipers, Actitis macularius (Charadriiformes), from Belize, Texas, and Montana.

    PubMed

    Canaris, Albert G; Kinsella, John M; Didyk, Andy S

    2009-12-01

    In total, 70 spotted sandpipers, Actitis macularius, were examined for helminth parasites; 47 from Belize, 18 from Texas, and 5 from Montana. The compound communities consisted of 10 species of helminths for Belize, 5 for Texas, and 6 for Montana, for a total of 17 different species. The most prevalent and abundant helminths for Belize were 3 microphallid trematodes, Paramaritremopsis solielangi, Levinseniella carteretensis, and Microphallus kinsellai; for Texas, the cestodes Kowalewskiella cingulifera and Choanotaenia cayennensis; and for Montana, the cestodes Anomotaenia hypoleuci and K. cingulifera. The cestode K. cingulifera was the only species recorded from all 3 localities. The cestode A. hypoleuci was the only specialist. Characteristics for helminth compound communities from the 3 localities were similar in terms of low mean species richness, medium diversity, and in uneven parasite distribution. Infracommunities were species poor for all 3 localities. For the Belize sample, only 6 of the 47 hosts harbored as many as 3 helminth species, and only 1 harbored more, at 5 species. Only 1 host harbored as many as 3 species in the sample from Texas, and a single host harbored a high of 4 species for Montana. There were no significant differences for mean species richness or mean abundance among the 3 localities. There were no significant differences for species richness or mean abundance between the combined freshwater sample from Texas and Montana and the marine sample from Belize. There were no significant positive or negative associations between pairs of helminth species.

  11. Isoflavonoids production in callus culture of Pueraria tuberosa, the Indian kudzu.

    PubMed

    Vaishnav, Kamlesh; Goyal, Shaily; Ramawat, K G

    2006-12-01

    Isoflavonoid contents of different plant parts and callus tissues of the Indian Kudzu, Pueraria tuberosa (Roxb.ex.Willd.) DC are presented. The initial cultures were slow growing, associated with browning of the tissues. The production of four isoflavonoids (puerarin, genistin, genistein and daidzein) in the callus cultures of P. tuberosa was studied by manipulating the plant growth regulators and sucrose concentration in the medium. Organogenesis was not recorded in callus on any of these treatments. Tuber and stem accumulated puerarin, a glycoside of daidzein, at high amounts, 0.65% and 0.054% respectively. However, the daidzein content of the callus tissues grown on Murashige and Skoog medium containing BA (20.9 microM) and sucrose (60 gl(-1)) was significantly higher (0.056%) than in vivo plant material (0.02%) and other comparable culture systems like Genista and Pueraria lobata.

  12. The Montana Small Schools Self-Evaluation Guide, 2000.

    ERIC Educational Resources Information Center

    Montana Small Schools Alliance, Helena.

    The Montana School Accreditation Standards require every Montana school to conduct a self-evaluation at least every 10 years. For some small Montana schools this has been an informal process and often more anecdotal than data driven. Using the Montana Statewide Education Profile as a model, a committee of county superintendents, teachers, and…

  13. Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation.

    PubMed

    Udomsuk, Latiporn; Jarukamjorn, Kanokwan; Tanaka, Hiroyuki; Putalun, Waraporn

    2011-02-01

    The effect of abiotic and biotic elicitors (methyl jasmonate, chitosan, salicylic acid, Agrobacterium, and yeast extract) at various concentrations on total isoflavonoid accumulation was studied in the hairy root cultures of Pueraria candollei. All elicitors stimulated isoflavonoid production. Yeast extract (0.5 mg/ml) was the most efficient giving total isoflavonoids at 60 ± 1 mg/g dry wt, which was 4.5-fold higher than control hairy roots on day 3 of elicitation.

  14. Anti-Endometriotic Effects of Pueraria Flower Extract in Human Endometriotic Cells and Mice

    PubMed Central

    Kim, Ji-Hyun; Woo, Jeong-Hwa; Kim, Hye Mi; Oh, Myung Sook; Jang, Dae Sik; Choi, Jung-Hye

    2017-01-01

    Pueraria flowers have been used as a vegetable and an ingredient for tea and jelly. In this study, we investigated the effects of Pueraria flower extract (PFE) on endometriosis, a common gynaecological disease characterised by local sterile inflammation of peritoneal cavity. PFE suppressed the adhesion of human endometriotic cells 11Z and 12Z to human mesothelial Met5A cells. In addition, PFE significantly inhibited the migration of 11Z and 12Z cells as shown by wound-healing and transwell migration assays. PFE reduced the protein and mRNA levels of matrix metalloproteinase (MMP)-2 and MMP-9 in endometriotic cells. Moreover, extracellular signal-regulated kinase (ERK)1/2 was activated by PFE treatment, and an ERK1/2 inhibitor, PD98059, significantly inhibited PFE-inhibited cell migration in endometriotic cells. Furthermore, PFE significantly suppressed endometriotic lesion formation in a mouse model. These data suggest that Pueraria flower is a potential anti-endometriotic agent for the inhibition of endometriotic cell adhesion, migration, and MMP expression. PMID:28264481

  15. NORTH ABSAROKA STUDY AREA, MONTANA.

    USGS Publications Warehouse

    Elliott, J.E.; Stotelmeyer, R.B.

    1984-01-01

    A mineral survey of the North Absaroka study area in Montana was conducted. The results of this survey indicate that parts of the area are extensively mineralized and that the area has potential for resources of gold, silver, copper, molybdenum, nickel, lead, zinc, platinum-group metals, uranium, iron, manganese, chromium, tungsten, and arsenic. Six areas of probable and substantiated mineral-resource potential were identified. The nature of the geologic terrain indicates that there is little likelihood for occurrence of oil, gas, coal, or geothermal resources.

  16. GALLATIN DIVIDE ROADLESS AREA, MONTANA.

    USGS Publications Warehouse

    Simons, Frank S.; Close, Terry J.

    1984-01-01

    A mineral-resource survey of the Gallatin Divide Roadless Area in the Gallatin Range of southwestern Montana was made. The area has probable and substantiated mineral-resource potential for phosphate rock, but most of the phosphate beds are thin, discontinuous, low grade, and deeply buried. Petrified wood is abundant but is scattered and of poor quality. Oil and gas resources are unlikely because possible productive structures are small and deeply eroded. The roadless area has little promise for the occurrence of other mineral or energy resources.

  17. Engineers without Borders at Montana State University: Student-Led Engagement and Transnational Collaboration

    ERIC Educational Resources Information Center

    Stein, Otto R.; Schmalzbauer, Leah

    2012-01-01

    The Montana State University student chapter of Engineers Without Borders USA is a student-managed partnership with the people of Khwisero, Kenya. The primary mission, to bring potable water and clean sanitation facilities to 61 primary schools and the surrounding communities of Khwisero, necessitates a long-term commitment to collaboration and…

  18. Montana Statewide Education Profile: K-12 Public Schools, School Year 1996-97.

    ERIC Educational Resources Information Center

    Nielson, Dori B.; Lamson, Joe; Love, Catherine; Quinlan, Madalyn

    This is Montana's first education profile. The measures in the profile, and the information they contain, provide a clear picture of education in the state. Public school education is provided by more than 460 school districts in this, the U.S.'s fourth largest state in terms of land. With only a few large communities, more than two-thirds of the…

  19. Fires in Idaho and Montana

    NASA Technical Reports Server (NTRS)

    2002-01-01

    2000 continues to be the worst fire season in the United States in decades. By August 8, 2000, fires in Montana and Idaho had burned more than 250,000 acres. Resources were stretched so thin that Army and Marine soldiers were recruited to help fight the fires. President Clinton visited Payette National Forest to lend moral support to the firefighters. Dense smoke from Idaho and western Montana is visible stretching all the way to North and South Dakota in this image from the Sea-viewing Wide Field-of-view Sensor (SeaWiFS). The image was taken on August 7, 2000. Although the primary mission of SeaWiFS is to measure the biology of the ocean, it also provides stunning color imagery of the Earth's surface. For more information about fires in the U.S., visit the National Interagency Fire Center. To learn more about using satellites to monitor fires, visit Global Fire Monitoring and New Technology for Monitoring Fires from Space in the Earth Observatory. Provided by the SeaWiFS Project, NASA/Goddard Space Flight Center, and ORBIMAGE

  20. Fires in Idaho and Montana

    NASA Technical Reports Server (NTRS)

    2002-01-01

    2000 continues to be the worst fire season in the United States in decades. By August 8, 2000, fires in Montana and Idaho had burned more than 250,000 acres. Resources were stretched so thin that Army and Marine soldiers were recruited to help fight the fires. President Clinton visited Payette National Forest to lend moral support to the firefighters. Dense smoke from Idaho and western Montana is visible stretching all the way to North and South Dakota in this image from the Sea-viewing Wide Field-of-view Sensor (SeaWiFS). The image was taken on August 7, 2000. Although the primary mission of SeaWiFS is to measure the biology of the ocean, it also provides stunning color imagery of the Earth's surface. For more information about fires in the U.S., visit the National Interagency Fire Center. To learn more about using satellites to monitor fires, visit Global Fire Monitoring and New Technology for Monitoring Fires from Space in the Earth Observatory. Provided by the SeaWiFS Project, NASA/Goddard Space Flight Center, and ORBIMAGE

  1. Task Force on Two-Year Education in Montana Interim Report to the Commissioner of Higher Education.

    ERIC Educational Resources Information Center

    Montana Commission for Higher Education Facilities, Helena.

    The Montana Commission of Higher Education put forth this report on the best practices in two-year colleges and operational recommendation in November 2002. The report describes the practices used in the best community colleges as the following: (1) responsiveness of the community college to the ever expanding needs of those they serve; (2) full…

  2. 78 FR 44187 - Montana Disaster # MT-00079

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-23

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Montana Disaster MT-00079 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... have been determined to be adversely affected by the disaster: Primary Counties: Blaine,...

  3. 76 FR 30010 - Montana Regulatory Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    .... Montana revised its program to clarify ambiguities and improve operational efficiency. DATES: Effective... implications. SMCRA delineates the roles of the Federal and State governments with regard to the regulation...

  4. Deer Lodge Valley investigations, western Montana

    SciTech Connect

    Wideman, C.J.; Sonderegger, J.; Crase, E.; Peterson, J.; Ruscetta, C.A.

    1982-07-01

    A review of the geothermal investigations conducted in the Deer Lodge Valley of Western Montana is briefly presented. Maps of the generalized geology and Bouguer gravity and graphs of selected geothermal gradients and resistivity sounding profiles are presented. (MJF)

  5. A Report on Traffic Safety and Montana's Children. 1999 Montana Special Report No. 1.

    ERIC Educational Resources Information Center

    Healthy Mothers, Healthy Babies--The Montana Coalition, Helena.

    This brief Kids Count report looks at major problems, available data, and some solutions for Montana's children as passengers in and drivers of vehicles on Montana's roads and highways. The report also presents information about adults' roles and responsibilities for preventing traffic accidents and protecting children. Facts presented in the…

  6. Case Study of a Service-Learning Partnership: Montana Tech and the Montana State Prison.

    ERIC Educational Resources Information Center

    Amtmann, John; Evans, Roberta; Powers, Jack

    2002-01-01

    As a service learning project, Montana Tech students deliver a wellness program for older inmates in Montana State Prison. Outcomes identified in student interviews included improved interpersonal skills (tact, diplomacy, communication, assertiveness) and opportunities to apply knowledge. Students recognized the value of the program for…

  7. Case Study of a Service-Learning Partnership: Montana Tech and the Montana State Prison.

    ERIC Educational Resources Information Center

    Amtmann, John; Evans, Roberta; Powers, Jack

    2002-01-01

    As a service learning project, Montana Tech students deliver a wellness program for older inmates in Montana State Prison. Outcomes identified in student interviews included improved interpersonal skills (tact, diplomacy, communication, assertiveness) and opportunities to apply knowledge. Students recognized the value of the program for…

  8. Montana Institute for Effective Teaching of American Indian Children (Missoula, Montana, 1992).

    ERIC Educational Resources Information Center

    Montana State Dept. of Public Instruction, Helena.

    This guide presents 11 American Indian study units developed by Montana teachers. Nine units are intended for intermediate or middle-school grades; two are suitable for prekindergarten through primary grades. The units contain information about various American Indian tribes, but focus on tribes of Montana. Many lessons include writing and…

  9. Montana's Indian Education. A University of Montana School of Journalism Special Report.

    ERIC Educational Resources Information Center

    Montana Univ., Missoula. School of Journalism.

    Originally presented in newspaper format, this report consists of 13 articles on American Indian education in Montana, written by journalism students at the University of Montana. The articles include: (1) "The Relentless Killing of a Culture" (David Zelio) which discusses the cultural genocide committed at boarding schools with the aim…

  10. A Report on Teen Pregnancy in Montana. 1996/97 Montana Special Report No. 2.

    ERIC Educational Resources Information Center

    Healthy Mothers, Healthy Babies--The Montana Coalition, Helena.

    This brief "Kids Count" report explores the impact of and factors that bear on teen pregnancy in Montana and ways to prevent teen pregnancy. Statistics and summaries are provided in the following areas: (1) live births to women under age 20, 1990-95; (2) Montana's unmarried teen births as compared to neighboring states; (3) number of…

  11. Isoflavonoid compounds extracted from Pueraria lobata suppress alcohol preference in a pharmacogenetic rat model of alcoholism.

    PubMed

    Lin, R C; Guthrie, S; Xie, C Y; Mai, K; Lee, D Y; Lumeng, L; Li, T K

    1996-06-01

    The extract from an edible vine, Pueraria lobata, has long been used in China to lessen alcohol intoxication. We have previously shown that daidzin, one of the major components from this plant extract, is efficacious in lowering blood alcohol levels and shortens sleep time induced by alcohol ingestion. This study was conducted to test the antidipsotropic effect of daidzin and two other major isoflavonoids, daidzein and puerarin, from Pueraria lobata administered by the oral route. An alcohol-preferring rat model, the selectively-bred P line of rats, was used for the study. All three isoflavonoid compounds were effective in suppressing voluntary alcohol consumption by the P rats. When given orally to P rats at a dose of 100 mg/kg/day, daidzein, daidzin, and puerarin decreased ethanol intake by 75%, 50%, and 40%, respectively. The decrease in alcohol consumption was accompanied by an increase in water intake, so that the total fluid volume consumed daily remained unchanged. The effects of these isoflavonoid compounds on alcohol and water intake were reversible. Suppression of alcohol consumption was evident after 1 day of administration and became maximal after 2 days. Similarly, alcohol preference returned to baseline levels 2 days after discontinuation of the isoflavonoids. Rats receiving the herbal extracts ate the same amounts of food as control animals, and they gained weight normally during the experiments. When administered orally, none of these compounds affected the activities of liver alcohol dehydrogenase and aldehyde dehydrogenase. Therefore, the reversal of alcohol preference produced by these compounds may be mediated via the CNS. Data demonstrate that isoflavonoid compounds extracted from Pueraria lobata is effective in suppressing the appetite for alcohol when taken orally, raising the possibility that other constituents of edible plants may exert similar and more potent actions.

  12. The effect of Pueraria mirifica on cytologic and urodynamic findings in ovariectomized rats.

    PubMed

    Manonai, Jittima; Seif, Christoph; Böhler, Georg; Jünemann, Klaus Peter

    2009-01-01

    To evaluate the effect of Pueraria mirifica on vaginal and urethral cytology, bladder pressure and capacity, residual urine, and leak point pressure in ovariectomized rats. Seventy-two adult, ovariectomized, female Sprague-Dawley rats were placed into one of four groups: control, estradiol, or 100 or 1,000 mg/kg of Pueraria mirifica (PM-100 and PM-1000, respectively). The vaginal and urethral smears were checked after 30 days of ovariectomy at pretreatment and at day 28 of treatment.A single cystometry, defined as the micturition interval, filling pressure, threshold pressure, micturition pressure, and voided volume, was performed. Peak bladder pressure was calculated for each leak point pressure measured at half bladder capacity by slowly and manually increasing abdominal pressure until a leak occurred, at which point external pressure was rapidly released. Leak point pressure was tested three times per rat. After 28 days of treatment, the estradiol, PM-100, and PM-1000 groups had significantly higher numbers of vaginal and urethral superficial cells compared with the control group (P < 0.05). Regarding the urodynamic parameters, the threshold pressure, micturition pressure, and leak point pressure were higher in the estradiol, PM-100, and PM-1000 groups compared with the control group (P < 0.05). The control, PM-100, and PM-1000 groups had the same values for micturition interval, bladder capacity, voided volume, and residual volume (P > 0.05) but lower values compared with the estradiol group (P < 0.05). Pueraria mirifica 100 and 1,000 mg/kg/day showed an estrogen-like effect on the vaginal and urethral epithelium of ovariectomized rats. They did not change bladder capacity and residual urine volume but increased leak point pressure according to urodynamic study.

  13. Phenolic compounds from Pueraria lobata protect PC12 cells against Aβ-induced toxicity.

    PubMed

    Choi, Yun-hyeok; Hong, Seong Su; Shin, Yu Su; Hwang, Bang Yeon; Park, So-Young; Lee, Dongho

    2010-10-01

    Bioassay-guided fractionation of the EtOAc-soluble extract of Pueraria lobata based on the inhibition of Aβ-induced toxicity in PC12 cells resulted in the isolation of four known active compounds, genistein (8), biochanin A (9), sissotrin (10), and puerol B (11). Of these, genistein (8) and biochanin A (9) exhibited potent neuroprotective effects with ED(50) values of 33.7 and 27.8 μM, respectively. In addition, a new coumestan, 2-(α,α-dimethylallyl)coumestrol (1) was isolated and characterized, but proved to be inactive, as were additional seven known compounds. The structure of new compound 1 was determined using spectroscopic techniques.

  14. Estimated water use in Montana in 2000

    USGS Publications Warehouse

    Cannon, M.R.; Johnson, Dave R.

    2004-01-01

    The future health and economic welfare of Montana's population is dependent on a continuing supply of fresh water. Montana's finite water resources are being stressed by increasing water withdrawals and instream-flow requirements. Various water managers in Montana need comprehensive, current, and detailed water-use data to quantify current stresses and estimate and plan for future water needs. This report summarizes selected water-use data for all of Montana's counties and stream basins to help meet those needs. In 2000, the citizens of Montana withdrew and used about 10,749 million gallons per day (Mgal/d) of water from Montana's streams and aquifers. Withdrawals from surface water were about 10,477 Mgal/d and withdrawals from ground water were about 272 Mgal/d. Agricultural irrigation accounted for about 10,378 Mgal/d or about 96.5 percent of total withdrawals for all uses. Withdrawals for public supply were about 136 Mgal/d, self-supplied domestic withdrawals were about 23 Mgal/d, self-supplied industrial withdrawals were about 61 Mgal/d, withdrawals for thermoelectric power generation were about 110 Mgal/d, and withdrawals for livestock were about 41 Mgal/d. Total consumptive use of water in 2000 was about 2,370 Mgal/d, of which about 2,220 Mgal/d (93.6 percent) was for agricultural irrigation. Instream uses of water included hydroelectric power generation and maintenance of instream flows for conservation of wildlife and aquatic life, and for public recreational purposes. In 2000, about 74,486 Mgal/d was used at hydroelectric plants for generation of about 11,591 gigawatt-hours of electricity. Evaporation from large water bodies, although not a classified water use, accounts for a large loss of water in some parts of the State. Net evaporation from Montana's 60 largest reservoirs and regulated lakes averaged about 891 Mgal/d.

  15. Observations on a Montana water quality proposal.

    SciTech Connect

    Veil, J. A.; Puder, M. G.

    2006-01-12

    In May 2005, a group of petitioners led by the Northern Plains Resource Council (NPRC) submitted a petition to revise water quality requirements to the Montana Board of Environmental Review (BER). Under Montana law, the BER had to consider the petition and either reject it or propose it as a new regulation. In September 2005, the BER announced proposed changes to the Montana water quality regulations. The proposal, which included almost the exact language found in the petition, was directed toward discharges of water from coal bed natural gas (CBNG) production. The key elements of the proposal included: (1) No discharges of CBNG water are allowed to Montana surface waters unless operators can demonstrate that injection to aquifers with the potential for later recovery of the water is not feasible. (2) When operators can demonstrate the injection is not feasible, the CBNG water to be discharged must meet very strict technology-based limits for multiple parameters. (3) The Montana water quality standards for the sodium adsorption ratio (SAR) and electrical conductivity (EC) would be evaluated using the 7Q10 flow (lowest 7-consecutive-day flow in a 10-year period) rather than a monthly flow that is currently used. (4) SAR and EC would be reclassified as ''harmful parameters'', thereby greatly restricting the ability for CBNG discharges to be allowed under Montana's nondegradation regulations. The proposed regulations, if adopted in their current form, are likely to substantially reduce the amount of CBNG production in Montana. The impact also extends to Wyoming CBNG production through much greater restrictions on water quality that must be met at the interstate border.

  16. 78 FR 45548 - Montana; Major Disaster and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-29

    ... SECURITY Federal Emergency Management Agency Montana; Major Disaster and Related Determinations AGENCY... declaration of a major disaster for the State of Montana (FEMA-4127-DR), dated July 10, 2013, and related... determined that the damage in certain areas of the State of Montana resulting from flooding during the period...

  17. 77 FR 50708 - Montana; Major Disaster and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-22

    ... SECURITY Federal Emergency Management Agency Montana; Major Disaster and Related Determinations AGENCY... declaration of a major disaster for the State of Montana (FEMA-4074-DR), dated August 2, 2012, and related... have determined that the damage in certain areas of the State of Montana resulting from a wildfire...

  18. 77 FR 49780 - Southern Montana Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-17

    ... Forest Service Southern Montana Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Southern Montana Resource Advisory Committee will meet in Columbus, Montana..., September 13, 2012 from 9:30 a.m. to 4 p.m. ADDRESSES: The meeting will be held at the Columbus Fire Hall...

  19. Board of Regents' Montana University System (MUS) Strategic Plan 2016

    ERIC Educational Resources Information Center

    Montana University System, 2016

    2016-01-01

    The Montana University System Strategic Plan is the primary planning document of the Board of Regents. The Plan sets forth an agenda for higher education in Montana by delineating the strategic directions, goals, and objectives that guide the Montana University System (MUS). In July 2006, after several years of study, public dialogue, and internal…

  20. 76 FR 37773 - Central Montana Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-28

    ... Forest Service Central Montana Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Central Montana Resource Advisory Committee will meet in Stanford, MT. The... the public. This will be the second official meeting of the Central Montana Resource Advisory...

  1. 76 FR 30094 - Central Montana Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ... Forest Service Central Montana Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Central Montana Resource Advisory Committee will meet in Stanford, MT. The... the public. This will be the second official meeting of the Central Montana Resource Advisory...

  2. 76 FR 20624 - Central Montana Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-13

    ... Forest Service Central Montana Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Central Montana Resource Advisory Committee will meet in Stanford, Montana... 109 Central Avenue, Stanford, MT. Written comments should be sent to Ron Wiseman, Lewis and Clark...

  3. 76 FR 49433 - Central Montana Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-10

    ... Forest Service Central Montana Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Central Montana Resource Advisory Committee will meet in Stanford, Montana... 109 Central Avenue, Stanford, MT. Written comments should be sent to Ron Wiseman, Lewis and Clark...

  4. Montana Library Laws, Rules, and Public Library Standards.

    ERIC Educational Resources Information Center

    Montana State Library, Helena.

    Chapter 1 of this handbook of Montana library laws, rules, and public library standards contains excerpts from the Constitution of Montana, including articles on property tax exemptions, educational goals and duties, and code of ethics. Montana library laws covering the following areas are presented in Chapter 2: publication and updating of the…

  5. [Simultaneous Determination of Five Active Components in Puerariae Lobatae Radix from Different Habitats by UHPLC].

    PubMed

    Zhao, Lu; Guan, Qin-xiao; Lu, Li; Guo, Xian-shuai; Liu, Guo-hong; Wang, Shu-mei

    2015-03-01

    To develop a UHPLC method for determination of 3'-hydroxy puerarin,puerarin, 3'-methoxy puerarin, daid- zin, and daidzein in Puerariae Lobatae Radix from different habitats. The analysis was carried out on an Agela Venusil MP C18 (100 mm x 2. 1 mm, 3 µm) column. The mobile phase was composed of 0. 1% formic acid and methanol with gradient elution. The de- tection wavelength was set at 250 nm. The standard curves of five components showed a good linearity in 12. 41 ~ 248. 24 ng(r =0. 9999), 58. 82 ~ 1 176. 47 ng(r =0. 9997), 12. 65 ~252. 94 ng(r=0. 9999), 12.14 ~ 242.82 ng(r=0. 9998), and 1. 82 ~ 36.30 ng(r =0. 9997), respectively. The average recoveries were 99. 03% ~ 100. 32%, and RSD values were 0. 26% ~ 1. 37%. This method is simple, quick,reproducible and can be used to control the quality of Puerariae Lobatae Radix.

  6. Development and validation of an HPLC method for quality control of Pueraria lobata flower.

    PubMed

    Bebrevska, Lidiya; Bravo, Luis; Vandervoort, Jo; Pieters, Luc; Vlietinck, Arnold; Apers, Sandra

    2007-12-01

    Pueraria lobata, also known as Kudzu (Japan) or Ge (China), is a medicinal plant widely used in Oriental traditional medicine. In this study the development, optimization and validation of an HPLC ethod for quality control of Pueraria flower plant material is presented. By means of this analytical method the three major compounds, i. e., the isoflavones tectorigenin 7- O-[beta- D-xylopyranosyl-(1 - 6)-beta- D-glucopyranoside], tectorigenin 7- O-beta- D-glucopyranoside and tectorigenin, were quantified, using the isoflavones genistin and genistein as external standards. The extraction procedure, the extraction solvent, the extraction yields and the HPLC conditions were evaluated and optimized. The samples were analyzed on an RP C18 column, and eluted with a binary system consisting of water and methanol using a linear gradient; detection was at 262 nm. Tectorigenin used in the recovery experiments was isolated and purified in the laboratory. The final method was fully validated according to the ICH guidelines in terms of linearity, precision and accuracy. The validation data showed that the precision, (RSD% (betweendays) of 3.1, 2.84 and 1.77 for the three major compounds, respectively), and the accuracy (recovery of 104.2 %) were acceptable. These validation results demonstrate the suitability of the method for the quality control of this crude drug.

  7. Isoorientin, a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa.

    PubMed

    Sumalatha, Manne; Munikishore, Rachakunta; Rammohan, Aluru; Gunasekar, Duvvuru; Kumar, Kotha Anil; Reddy, Kakularam Kumar; Azad, Rajaram; Reddanna, Pallu; Bodo, Bernard

    2015-10-01

    Bioassay-guided fraction of the methanol extract of the roots of Pueraria tuberose DC yielded puerarin, an isoflavone C-glycoside (PT-1), isoorientin, a flavone C-glycoside (PT-2) and mangiferin, a xanthone C-glycoside (PT-3). The extracts and the isolated compounds were screened for potent anti-inflammatory components inhibiting the cyclooxygenases (COX-1 and COX-2) and 5-lipoxygenase (5-LOX), the target enzymes of inflammation, by employing spectroscopic/polorographic methods. Among these, isoorientin was found to be a potent inhibitor of COX-2with an IC50 value of 39 μM. Docking studies were carried out to understand the interactions of isorientin (PT-2) with COX-2.The structures of the isolates were determined by mass spectrometry and 2D-NMR techniques including HSQC, HMBC, NOESY and 1H-1H COSY experiments. Although isoorientin and mangiferin have been reported from several plant sources, this is the first report of their isolation from a Pueraria species.

  8. [Microscopic anatomy of abnormal structure in root tuber of Pueraria lobata].

    PubMed

    Duan, Hai-yan; Cheng, Ming-en; Peng, Hua-sheng; Zhang, He-ting; Zhao, Yu-jiao

    2015-11-01

    Puerariae Lobatae Radix, also known as Gegen, is a root derived from Pueraria lobata. Based on field investigation and the developmental anatomy of root tuber, we have elucidated the relationship between the growth of root tuber and the anomalous structure. The results of analysis showed that the root system of P. lobata was developed from seed and adventitious root and there existed root tuber, adventitious root and conductive root according to morphology and function. The root tuber was developed from adventitious root, its secondary structure conformed to the secondary structure of dicotyledon's root. With the development of root, the secondary phloem of root tuber appeared abnormal vascular tissue, which was distributed like ring in the outside of secondary vascular tissue. The root tuber might have 4-6 concentric circular permutation abnormal vascular tissuelobate, and was formed by the internal development of abnormal vascular tissue. The xylem and phloem of abnormal vascular tissue were the main body of the root tuber. The results reveal the abnormal anatomical structure development of P. lobata, also provides the theoretical basis for reasonable harvest medicinal parts and promoting sustainable utilization of resources of P. lobata.

  9. A novel and effective chromatographic approach to the separation of isoflavone derivatives from Pueraria lobata.

    PubMed

    Fu, Jiang; Jing, Wenguang; Wang, Weihao; Chen, Sha; Zhang, Jun; Liu, An

    2015-03-05

    A novel and effective chromatographic approach to the separation and purification of isoflavone compounds from Pueraria lobata is described. The method is based on flash chromatography (FC), coupled to preparative high performance liquid chromatography (prep-HPLC) via a six-way valve. The FC step comprised tandem reversed phase columns, pre-packed with MCI gel (Mitsubishi Chemical Corp., Tokyo, Japan) and C18 (Fuji Silysia Chemical Ltd, Osaka, Japan) resin, respectively, and was designed to separate a crude Pueraria lobata extract into several preliminary fractions. Fractions containing the target compounds were then directly injected via the six-way valve into prep-HPLC columns, without further treatment, for final isolation and purification. Nine isoflavonoids were successfully isolated, three through an online mode and the other six through an offline mode. The purities of all compounds exceeded 95.0%, as determined by HPLC with an UV-vis photodiode array detector. The convenience, low solvent consumption, and time-saving advantages of this method offer an attractive and promising approach to the isolation of natural products.

  10. Effects of Dietary Isoflavones from Puerariae Radix on Lipid and Bone Metabolism in Ovariectomized Rats

    PubMed Central

    Lim, Dong Wook; Kim, Jae Goo; Kim, Yun Tai

    2013-01-01

    Puerariae radix, the dried root of Pueraria lobata Ohwi, is one of the earliest and most important edible crude herbs used for various medical purposes in oriental medicine. This study evaluated the metabolic effects of total isoflavones from P. lobata (PTIF) in ovariectomized (OVX) rats. The OVX rats were divided into four groups treated with distilled water, 17β-estradiol (E2 10 μg/kg, once daily, i.p.) and PTIF (30 and 100 mg/kg, once daily, p.o.) for eight weeks. The treatments with high-dose PTIF significantly decreased the bone mineral density (BMD) loss in the femur and inhibited the increase in body weight and lipoprotein levels compared to the OVX-control group without elevating the serum levels of the liver enzymes, aspartate aminotransferase (AST) and alanine transaminase (ALT). Furthermore, PTIF exhibits a hepatoprotective effect in OVX-induced hepatic steatosis, indicated with reduced hepatic lipid contents. Taken together, our findings suggest that PTIF may be useful for controlling lipid and bone metabolism, at least in OVX rats. Further research is necessary to determine whether PTIF will have the same effects in humans. PMID:23867712

  11. Effects of dietary isoflavones from Puerariae radix on lipid and bone metabolism in ovariectomized rats.

    PubMed

    Lim, Dong Wook; Kim, Jae Goo; Kim, Yun Tai

    2013-07-17

    Puerariae radix, the dried root of Pueraria lobata Ohwi, is one of the earliest and most important edible crude herbs used for various medical purposes in oriental medicine. This study evaluated the metabolic effects of total isoflavones from P. lobata (PTIF) in ovariectomized (OVX) rats. The OVX rats were divided into four groups treated with distilled water, 17β-estradiol (E2 10 μg/kg, once daily, i.p.) and PTIF (30 and 100 mg/kg, once daily, p.o.) for eight weeks. The treatments with high-dose PTIF significantly decreased the bone mineral density (BMD) loss in the femur and inhibited the increase in body weight and lipoprotein levels compared to the OVX-control group without elevating the serum levels of the liver enzymes, aspartate aminotransferase (AST) and alanine transaminase (ALT). Furthermore, PTIF exhibits a hepatoprotective effect in OVX-induced hepatic steatosis, indicated with reduced hepatic lipid contents. Taken together, our findings suggest that PTIF may be useful for controlling lipid and bone metabolism, at least in OVX rats. Further research is necessary to determine whether PTIF will have the same effects in humans.

  12. Anti-inflammatory effects of total isoflavones from Pueraria lobata on cerebral ischemia in rats.

    PubMed

    Lim, Dong Wook; Lee, Changho; Kim, In-Ho; Kim, Yun Tai

    2013-08-28

    Puerariae radix, the dried root of Pueraria lobata Ohwi, is one of earliest and most important edible crude herbs used for various medical purposes in Oriental medicine. The aim of the present study was to determine the anti-inflammatory effects of Total Isoflavones from P. lobata (TIPL), which contains the unique isoflavone puerarin, in ischemia in vivo models. Oral administration of TIPL (100 mg/kg) reduced the brain infarct volume and attenuated ischemia-induced cyclooxygenase-2 (COX-2) up-regulation at 2 days after middle cerebral artery occlusion (MCAo) in rats. Moreover, TIPL reduced activation of glial fibrillary acid protein (GFAP) and CD11b antibody (OX-42) at 7 days after MCAo in hippocampal CA1 region. These results show that TIPL can protect the brain from ischemic damage after MCAo. Regarding the immunohistochemical study, the effects of TIPL may be attributable to its anti-inflammatory properties by the inhibition of COX-2 expression, astrocyte expression, and microglia.

  13. Minerals yearbook, 1991: Montana. Annual report

    SciTech Connect

    Minarik, R.J.; McCulloch, R.B.

    1993-07-01

    The report has been prepared under a Memorandum of Understanding between the Bureau of Mines, U.S. Department of the Interior, and the Montana Bureau of Mines and Geology for collecting information on all nonfuel minerals. Montana's 1991 nonfuel mineral production value was $590 million, an increase of 3% from that of 1990. Gains in the production value of portland cement, copper, and gold more than offset the decrease in values of molybdenum, platinum-group metals, silver, crushed stone, and zinc. Metallic minerals-copper, gold, iron ore, lead, molybdenum, platinum-group metals, silver, and zinc-accounted for more than 82% of Montana's total nonfuel mineral production value. The State ranked 17th nationally in value compared with 19th in 1990.

  14. Symbiotic N2-Fixation Estimated by the 15N Tracer Technique and Growth of Pueraria phaseoloides (Roxb.) Benth. Inoculated with Bradyrhizobium Strain in Field Conditions

    PubMed Central

    Sarr, Papa Saliou; Okon, Judith Wase; Begoude, Didier Aime Boyogueno; Araki, Shigeru; Ambang, Zachée; Shibata, Makoto; Funakawa, Shinya

    2016-01-01

    This field experiment was established in Eastern Cameroon to examine the effect of selected rhizobial inoculation on N2-fixation and growth of Pueraria phaseoloides. Treatments consisted of noninoculated and Bradyrhizobium yuanmingense S3-4-inoculated Pueraria with three replications each. Ipomoea batatas as a non-N2-fixing reference was interspersed in each Pueraria plot. All the twelve plots received 2 gN/m2 of 15N ammonium sulfate 10% atom excess. At harvest, dry matter yields and the nitrogen derived from atmospheric N2-fixation (%Ndfa) of inoculated Pueraria were significantly (P < 0.05) higher (81% and 10.83%, resp.) than those of noninoculated Pueraria. The inoculation enhanced nodule dry weight 2.44-fold. Consequently, the harvested N significantly (P < 0.05) increased by 83% in inoculated Pueraria, resulting from the increase in N2-fixation and soil N uptake. A loss of 55 to 60% of the N fertilizer was reported, and 36 to 40% of it was immobilized in soil. Here, we demonstrated that both N2-fixing potential of P. phaseoloides and soil N uptake are improved through field inoculations using efficient bradyrhizobial species. In practice, the inoculation contributes to maximize N input in soils by the cover crop's biomass and represent a good strategy to improve soil fertility for subsequent cultivation. PMID:26904363

  15. 30 CFR 926.25 - Approval of Montana abandoned mine land reclamation plan amendments.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 3 2012-07-01 2012-07-01 false Approval of Montana abandoned mine land... STATE MONTANA § 926.25 Approval of Montana abandoned mine land reclamation plan amendments. (a) Montana... to Montana Abandoned Mine Land Inventory; emergency response reclamation program; organizational...

  16. 30 CFR 926.25 - Approval of Montana abandoned mine land reclamation plan amendments.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 3 2014-07-01 2014-07-01 false Approval of Montana abandoned mine land... STATE MONTANA § 926.25 Approval of Montana abandoned mine land reclamation plan amendments. (a) Montana... to Montana Abandoned Mine Land Inventory; emergency response reclamation program; organizational...

  17. 30 CFR 926.25 - Approval of Montana abandoned mine land reclamation plan amendments.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 3 2013-07-01 2013-07-01 false Approval of Montana abandoned mine land... STATE MONTANA § 926.25 Approval of Montana abandoned mine land reclamation plan amendments. (a) Montana... to Montana Abandoned Mine Land Inventory; emergency response reclamation program; organizational...

  18. 75 FR 36436 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-25

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Drive, Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009. SUPPLEMENTARY... Plains Region, Montana Area Office, Billings, Montana, and was necessary to determine the boundaries...

  19. The Northern Cheyenne Tribe and Energy Development in Southeastern Montana. Volume I: Social, Cultural, and Economic Investigations.

    ERIC Educational Resources Information Center

    Nordstrom, Jean; And Others

    Although there are no easy answers to the economic problems faced by the Northern Cheyenne in Southeast Montana, any economic development plans should stress long-term tribal self-sufficiency, serve community needs, and consider the Cheyenne culture. Differentiation theory is appropriate for understanding the local rural area and the special place…

  20. From Threshers to Thrashers: In Montana, 4-H Takes a Modern Spin To Engage "Blue-Ribbon Kids."

    ERIC Educational Resources Information Center

    Linik, Joyce Riha

    2002-01-01

    A 4-H skateboard club in Bozeman, Montana offers kids community service and educational opportunities as well as something to do after school. Adult and college-student volunteers teach a skateboarding curriculum and help kids fix boards and design ramps. Club members have learned public speaking, fundraising, city planning, and civic involvement…

  1. 75 FR 55713 - Air Quality Implementation Plans; Montana; Attainment Plan for Libby, MT PM2.5

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-14

    ..., avoiding the use of profanity or personal threats. h. Make sure to submit your comments by the comment.... Background Libby, Montana, a small rural community, is located in Lincoln County in the northwestern part of... determined that in light of their small contribution to PM 2.5 nonattainment, existing federal...

  2. 78 FR 63911 - Montana Regulatory Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-25

    ... Office of Surface Mining Reclamation and Enforcement 30 CFR Part 926 ; Docket ID: OSM-2013-0009... AGENCY: Office of Surface Mining Reclamation and Enforcement, Interior. ACTION: Proposed rule; public...'') under the Surface Mining Control and Reclamation Act of 1977 (``SMCRA'' or ``the Act''). Montana...

  3. Montana Proprietary Schools. Staff Report No. 9.

    ERIC Educational Resources Information Center

    Montana Commission on Post-Secondary Education, Helena.

    The current status of proprietary school education in Montana and the relationship between state-federal agencies and proprietary schools were studied. Data and information were collected via questionnaires, meetings, personal interviews, personal observations, and input from concerned individuals. Section 2 of the report shows the present status,…

  4. 75 FR 81112 - Montana Regulatory Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-27

    ...: Interseeding and Supplemental Planting of Tree and Shrub Seedlings (III.A.); Mechanical Practices, Supplemental... Shrub Seedlings. Montana proposes to add the following language regarding Interseeding and Supplemental... support of the proposed practices for Interseeding and Supplemental Planting of Tree and Shrub Seedlings...

  5. Montana's forest resources, 2003-2009

    Treesearch

    Jim Menlove; John D. Shaw; Michael T. Thompson; Chris Witt; Michael C. Amacher; Todd A. Morgan; Colin Sorenson; Chelsea McIver; Charles Werstak

    2012-01-01

    This report presents a summary of the most recent inventory information for Montana's forest lands. The report includes descriptive highlights and tables of area, number of trees, biomass, volume, growth, mortality, and removals. Most of the tables are organized by forest type group, species group, diameter class, or owner group. The report also describes...

  6. 40 CFR 81.417 - Montana.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 88-577 USDA-FS U. L. Bend Wild 20,890 94-557 USDI-FWS Yellowstone NP 2 167,624 (3) USDI-NPS 1 Selway... Montana. 2 Yellowstone National Park, 2,219,737 acres overall, of which 2,020,625 acres are in...

  7. 40 CFR 81.417 - Montana.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 88-577 USDA-FS U. L. Bend Wild 20,890 94-557 USDI-FWS Yellowstone NP 2 167,624 (3) USDI-NPS 1 Selway... Montana. 2 Yellowstone National Park, 2,219,737 acres overall, of which 2,020,625 acres are in...

  8. 40 CFR 81.417 - Montana.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 88-577 USDA-FS U. L. Bend Wild 20,890 94-557 USDI-FWS Yellowstone NP 2 167,624 (3) USDI-NPS 1 Selway... Montana. 2 Yellowstone National Park, 2,219,737 acres overall, of which 2,020,625 acres are in...

  9. 40 CFR 81.417 - Montana.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 88-577 USDA-FS U. L. Bend Wild 20,890 94-557 USDI-FWS Yellowstone NP 2 167,624 (3) USDI-NPS 1 Selway... Montana. 2 Yellowstone National Park, 2,219,737 acres overall, of which 2,020,625 acres are in...

  10. Glauconite from the precambrian belt series, Montana

    USGS Publications Warehouse

    Gulbrandsen, R.A.; Goldich, S.S.; Thomas, H.H.

    1963-01-01

    Glauconite from the upper part of the Missoula Group of the Belt Series, Flathead County, Montana, has been dated at 1070 million years by potassium-argon and rubidium-strontium analyses. This is the first glauconite of Precambrian age reported in North America.

  11. Making FPS Research Fun in Montana.

    ERIC Educational Resources Information Center

    Stetzner, Kate

    1991-01-01

    Gifted and talented fifth and sixth grade students participating in Montana's Future Problem Solving Program are developing their information retrieval skills as they use electronic equipment to retrieve research literature and use a hypercard authoring system to record and manipulate their findings. (JDD)

  12. 77 FR 73965 - Montana Regulatory Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-12

    ... Section 503(a) of the Act permits a State to assume primacy for the regulation of surface coal mining and... Office of Surface Mining Reclamation and Enforcement 30 CFR Part 926 Montana Regulatory Program AGENCY: Office of Surface Mining Reclamation and Enforcement, Interior. ACTION: Proposed rule;...

  13. Montana Kids Count 1996 Data Book.

    ERIC Educational Resources Information Center

    Healthy Mothers, Healthy Babies--The Montana Coalition, Helena.

    This 1996 KIDS COUNT data book presents comparative data on child well-being for each county in Montana and for the state as a whole. Data in the county profiles, which comprise the bulk of the report, are grouped into: background facts (demographic, mental health, education, security, and income support information); charts showing changes in…

  14. Directory of Indian Education Programs in Montana.

    ERIC Educational Resources Information Center

    Montana State Office of Public Instruction, Helena.

    This directory compiles information on Montana agencies, schools, colleges, and programs concerned with American Indian education. The first section covers educational agencies, associations, and resources. A brief annotated bibliography describes 10 books for educators. Contact information is provided for the Board of Public Education, the…

  15. Public Education Policy Issues in Montana.

    ERIC Educational Resources Information Center

    Hitz, Randy, Ed.; Chambers, Keith, Ed.

    This document contains articles that address six major issues affecting Montana public education. The issues were selected by an advisory group comprised of representatives from professional education organizations, state agencies, the legislature, and business. Each of the articles, written by members of the advisory group, contains an executive…

  16. Montana Advanced Biofuels Great Falls Approval

    EPA Pesticide Factsheets

    This November 20, 2015 letter from EPA approves the petition from Montana Advanced Biofuels, LLC, Great Falls facility, regarding ethanol produced through a dry mill process, qualifying under the Clean Air Act for advanced biofuel (D-code 5) and renewable

  17. Coach Education Online: The Montana Model

    ERIC Educational Resources Information Center

    Stewart, Craig

    2006-01-01

    Coach education is important, but expensive--both in cost and time to public and private athletic programs. To provide basic coach education to coaches, new, innovative, inexpensive approaches must be developed. Joint efforts between state high school associations and colleges and universities can meet those needs. The "Montana approach"…

  18. 40 CFR 81.417 - Montana.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 88-577 USDA-FS U. L. Bend Wild 20,890 94-557 USDI-FWS Yellowstone NP 2 167,624 (3) USDI-NPS 1 Selway... Montana. 2 Yellowstone National Park, 2,219,737 acres overall, of which 2,020,625 acres are in Wyoming...

  19. Coach Education Online: The Montana Model

    ERIC Educational Resources Information Center

    Stewart, Craig

    2006-01-01

    Coach education is important, but expensive--both in cost and time to public and private athletic programs. To provide basic coach education to coaches, new, innovative, inexpensive approaches must be developed. Joint efforts between state high school associations and colleges and universities can meet those needs. The "Montana approach"…

  20. Distance Learning Offered in Rural Montana

    ERIC Educational Resources Information Center

    Bullard, Julie; Gilliard, Jennifer L.

    2004-01-01

    Due to increasing demands from the field, the University of Montana-Western (UM-W) recently began to offer a bachelor's degree in Early Childhood Education that builds upon the associate degree. Students complete their general education credits, early childhood specialty courses, and an area of special emphasis. Courses are offered online or…

  1. Montana's forest resources. Forest Service resource bulletin

    SciTech Connect

    Conner, R.C.; O'Brien, R.A.

    1993-09-01

    The report includes highlights of the forest resource in Montana as of 1989. Also the study describes the extent, condition, and location of the State's forests with particular emphasis on timberland. Includes statistical tables, area by land classes, ownership, and forest type, growing stock and sawtimber volumes, growth, mortality, and removals for timberland.

  2. Montana Health Education Curriculum Planning Guide.

    ERIC Educational Resources Information Center

    Montana State Dept. of Public Instruction, Helena.

    This guide is intended to serve administrators, curriculum directors, and teachers as a resource to develop or enhance the existing health education curriculum. After two introductory chapters that provide an overview of the Montana health education curriculum and its 10 content areas, the document provides guidelines for high school, junior high,…

  3. Montana Curriculum Guidelines for Distributive Education. Revised.

    ERIC Educational Resources Information Center

    Harris, Ron, Ed.

    These distributive education curriculum guidelines are intended to provide Montana teachers with teaching information for 11 units. Units cover introduction to marketing and distributive education, human relations and communications, operations and control, processes involved in buying for resale, merchandise handling, sales promotion, sales and…

  4. The Montana Behavioral Initiative: A Statewide Response.

    ERIC Educational Resources Information Center

    Fishbaugh, Mary Susan E.; Furshong, Joe

    1998-01-01

    The Montana Behavioral Initiative (MBI) is a staff development program created to stem school violence. Program components include summer institutes, site teams, ongoing technical assistance, interagency cooperation, program evaluation, information dissemination, and governance by the MBI council. MBI has expanded to include nearly 100 Montana…

  5. Distance Learning Offered in Rural Montana

    ERIC Educational Resources Information Center

    Bullard, Julie; Gilliard, Jennifer L.

    2004-01-01

    Due to increasing demands from the field, the University of Montana-Western (UM-W) recently began to offer a bachelor's degree in Early Childhood Education that builds upon the associate degree. Students complete their general education credits, early childhood specialty courses, and an area of special emphasis. Courses are offered online or…

  6. Made in Montana: Entrepreneurial Home Economics.

    ERIC Educational Resources Information Center

    Goetting, Marsha A.; Muggli, Gayle Y.

    1988-01-01

    Reports results from a survey of 13 Montana home economists who each started a small business. Information is included on types of businesses the women had started, income, personal characteristics, reasons for starting a business, its impact on family concerns, marketing, obstacles to success, and resources. (CH)

  7. Comparative analysis of the chemical constituents of two varieties of Pueraria candollei.

    PubMed

    Yusakul, Gorawit; Putalun, Waraporn; Udomsin, Orapin; Juengwatanatrakul, Thaweesak; Chaichantipyuth, Chaiyo

    2011-03-01

    A high-performance liquid chromatography (HPLC) method was developed to determine the contents of miroestrol and deoxymiroestrol in the tubers of Pueraria candollei var. mirifica and P. candollei var. candollei. The linear detection ranges were 0.78-25.00 μg/mL for miroestrol and 1.56-25.00 μg/mL for deoxymiroestrol. The limit of detection (LOD) and limit of quantification (LOQ) were 0.2 and 0.78 μg/mL, respectively, for miroestrol and 0.78 and 1.56 μg/mL, respectively, for deoxymiroestrol. Our results suggest that both varieties of P. candollei can produce miroestrol and deoxymiroestrol and that the developed HPLC method can be applied for quality control of plants and their products. Copyright © 2010 Elsevier B.V. All rights reserved.

  8. Constituents of the roots of Pueraria lobata inhibit formation of advanced glycation end products (AGEs).

    PubMed

    Kim, Jong Min; Lee, Yun Mi; Lee, Ga Young; Jang, Dae Sik; Bae, Ki Hwan; Kim, Jin Sook

    2006-10-01

    Two isoflavone C-glucosides, puerarin (1) and PG-3 (2), a but-2-enolide, (+/-)-puerol B (3), two isoflavone O-glucosides, daidzin (4) and genistin (5), and three pterocarpans, (-)-medicarpin (6), (-)-glycinol (7) and (-)-tuberosin (8), were isolated from a MeOH extract of the roots of Pueraria lobata, using an in vitro bioassay based on the inhibition of the formation of advanced glycation end products (AGEs) to monitor chromatographic fractionation. The structures of 1-8 were determined by spectroscopic data interpretation, particularly by 1D- and 2D-NMR studies, and by comparison of these data with values in the literature. All of the isolates (1-8) were evaluated for their inhibitory activity on AGEs formation in vitro. Of these, puerarin (1), PG-3 (2), and (+/-)-puerol B (3) exhibited more potent inhibitory activity than the positive control aminoguanidine.

  9. Intestinal bacteria activate estrogenic effect of main constituents puerarin and daidzin of Pueraria thunbergiana.

    PubMed

    Park, Eun-Kyung; Shin, Jieun; Bae, Eun-Ah; Lee, Young-Chul; Kim, Dong-Hyun

    2006-12-01

    To understand the relationship between the metabolites and estrogenic activity of the main isoflavones puerarin and daidzin of the rhizome of Pueraria thunbergiana (PT, family Leguminosae), PT and its isoflavones were transformed by human intestinal bacteria and their estrogenic effects were investigated. All human fecal specimens hydrolyzed puerarin and daidzin to daidzein, but their hydrolyzing activities varied depending on the individuals. All intestinal bacteria isolated from human also hydrolyzed daidzin to daidzein, but a few bacteria transformed puerarin to daidzein. When the estrogenic effect of PT, puerarin and daidzin was compared with those of their metabolites, the metabolites more potently increased proliferation of MCF-7 cells than PT, puerarin and daidzin. The metabolite daidzein also potently increased estrogen-response c-fos mRNA and PR protein expressions. These findings suggest that intestinal bacteria, which can hydrolyze puerarin and/or daidzin, may activate a potent estrogenic activity of PT.

  10. Antioxidant and anti-fatigue activities of flavonoids from Puerariae radix.

    PubMed

    Xiaoming, Wang; Ling, Lei; Jinghang, Zhang

    2012-01-01

    This study evaluated the antioxidant and anti-fatigue activities of flavonoids from Puerariae radix (FPR). In vitro antioxidant activities of FPR were investigated through hydroxyl and superoxide radical scavenging activities. In vivo anti-fatigue activity of FPR was investigated through loaded swimming exercise of mice. Results showed that FPR had not only in vitro antioxidant activities, but also an in vivo anti-fatigue activity in mice. FPR possessed superoxide and hydroxyl radical scavenging activity in in vitro experimental studies. In vivo experimental studies, FPR could evidently extend exhaustive swimming time of mice, inhibit the increase of blood lactic acid (BLA), decrease serum urea nitrogen (BUN) and malondialdehyde (MDA) contents, promote increases in the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) of mice after swimming. The results provided an important basis for developing the FPR as a novel antioxidant and anti-fatigue compound.

  11. A glycoside flavonoid in Kudzu (Pueraria lobata): identification, quantification, and determination of antioxidant activity.

    PubMed

    Lau, Ching S; Carrier, Danielle J; Beitle, Robert R; Howard, Luke R; Lay, Jackson O; Liyanage, Rohana; Clausen, Edgar C

    2005-01-01

    Kudzu (Pueraria lobata) foliage has been touted as a possible energy crop. High-performance liquid chromatography and mass spectrometry analysis of the methanolic kudzu foliage extracts confirmed the presence of robinin (kaempferol-3-O-robinoside-7-O-rhamnoside). Robinin accounted for 0.65 +/- 0.16% (dry basis) of kudzu biomass. Fast performance liquid chromatography (FPLC) was employed to fractionate robinin from the crude extract. The antioxidant capacity of robinin was evaluated by an oxygen radical absorbance capacity (ORAC) assay. The ORAC values of pure standard were compared with those of the extract fractions. One milligram of the FPLC-fractionated robinin generated an ORAC value of 5.15 +/- 2.00 micromol/mg of Trolox, whereas 1 mg of pure robinin generated an ORAC value of 12.34 +/- 0.45 micromol/mg of Trolox. Because of its antioxidant properties, robinin may be a flavonoid worth extracting prior to energy production.

  12. Potential Skin Regeneration Activity and Chemical Composition of Absolute from Pueraria thunbergiana Flower.

    PubMed

    Kim, Do-Yoon; Won, Kyung-Jong; Hwang, Dae-Il; Yoon, Seok Won; Lee, Su Jin; Park, Joo-Hoon; Yoon, Myeong Sik; Kim, Bokyung; Lee, Hwan Myung

    2015-11-01

    The flower of Pueraria thunbergiana BENTH (PTBF) contains isoflavonoids and essential oil components. It has many biological and pharmacological activities, including anti-diabetes, anti-oxidant, and weight loss. However, its effect on skin regeneration remains unknown. In the present study, we isolated the absolute from PTBF through solvent extraction and determined the role of the absolute on skin regeneration-associated responses in human epidermal-keratinocytes (HaCats). The PTBF absolute, which contained 10 compounds, stimulated migration and proliferation and increased the phosphorylation of serine/threonine-specific protein kinase and extracellular signal-regulated kinasel/2 in HaCats. It induced type I and IV collagen synthesis in HaCats. In addition, treatment with PTBF absolute resulted in increased sprout outgrowth in HaCats. These findings suggest that PTBF absolute may participate in skin regeneration, probably through promotion of migration, proliferation, and collagen synthesis.

  13. Bioactive isoflavones from Pueraria lobata root and starch: Different extraction techniques and carbonic anhydrase inhibition.

    PubMed

    Mocan, Andrei; Carradori, Simone; Locatelli, Marcello; Secci, Daniela; Cesa, Stefania; Mollica, Adriano; Riga, Simona; Angeli, Andrea; Supuran, Claudiu T; Celia, Christian; Di Marzio, Luisa

    2017-08-12

    Kudzu, the dried root of an important edible plant (Pueraria lobata), is used in Traditional Chinese Medicine for the important nutritional value strictly related to its isoflavone derivatives. These compounds characterize the quality of kudzu contained in different preparations, as pharmaceutical ingredient as well as dietary/food supplement (e.g. starch). The optimization of the isoflavones recovery, monitored by HPLC-PDA, through different innovative and conventional extraction techniques, e.g. microwave-assisted, ultrasound-assisted and conventional extraction, represented a suitable challenge in food industry and natural products evaluation. The impact on the isoflavone extraction by using an ionic liquid-assisted procedure was also considered. Furthermore, the inhibitory activity of the most representative isoflavones, isolated from kudzu, was evaluated using four isoforms (I, II, IX and XII) of human carbonic anhydrase (hCA) due to their role in several physiopathological processes. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Production of isoflavonoids in callus cultures of Pueraria candollei var. mirifica.

    PubMed

    Udomsuk, Latiporn; Jarukamjorn, Kanokwan; Tanaka, Hiroyuki; Putalun, Waraporn

    2009-01-01

    Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 +/- 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 +/- 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem explants of P. candollei var. mirifica.

  15. De novo transcriptome sequencing in Pueraria lobata to identify putative genes involved in isoflavones biosynthesis.

    PubMed

    Wang, Xin; Li, Shutao; Li, Jia; Li, Changfu; Zhang, Yansheng

    2015-05-01

    Using Illumina sequencing technology, we have generated the large-scale transcriptome sequencing data and indentified many putative genes involved in isoflavones biosynthesis in Pueraria lobata. Pueraria lobata, a member of the Leguminosae family, is a traditional Chinese herb which has been used since ancient times. P. lobata root has extensive clinical usages, because it contains a rich source of isoflavones, including daidzin and puerarin. However, the knowledge of isoflavone metabolism and the characterization of corresponding genes in such a pathway remain largely unknown. In this study, de novo transcriptome of P. lobata root and leaf was sequenced using the Solexa sequencing platform. Over 140 million high-quality reads were assembled into 163,625 unigenes, of which about 43.1% were aligned to the Nr protein database. Using the RPKM (reads per kilo bases per million reads) method, 3,148 unigenes were found to be upregulated, and 2,011 genes were downregulated in the leaf as compared to those in the root. Towards a further understanding of these differentially expressed genes, Gene ontology enrichment and metabolic pathway enrichment analyses were performed. Based on these results, 47 novel structural genes were identified in the biosynthesis of isoflavones. Also, 22 putative UDP glycosyltransferases and 45 O-methyltransferases unigenes were identified as the candidates most likely to be involved in the tailoring processes of isoflavonoid downstream pathway. Moreover, MYB transcription factors were analyzed, and 133 of them were found to have higher expression levels in the roots than in the leaves. In conclusion, the de novo transcriptome investigation of these unique transcripts provided an invaluable resource for the global discovery of functional genes related to isoflavones biosynthesis in P. lobata.

  16. Cost-Effectiveness Analysis of the Residential Provisions of the 2015 IECC for Montana

    SciTech Connect

    Mendon, Vrushali V.; Zhao, Mingjie; Taylor, Zachary T.; Poehlman, Eric A.

    2016-02-15

    The 2015 IECC provides cost-effective savings for residential buildings in Montana. Moving to the 2015 IECC from the 2014 Montana State Code base code is cost-effective for residential buildings in all climate zones in Montana.

  17. Consumption of Pueraria flower extract reduces body mass index via a decrease in the visceral fat area in obese humans.

    PubMed

    Kamiya, Tomoyasu; Takano, Akira; Matsuzuka, Yuki; Kusaba, Nobutaka; Ikeguchi, Motoya; Takagaki, Kinya; Kondo, Kazuo

    2012-01-01

    In Japan, kudzu is a familiar plant, well-known as an ingredient in the Japanese-style confections kudzu-kiri and kudzu-mochi. In this study, we focused on the flower of kudzu (Pueraria thomsonii) and conducted a clinical trial to investigate the effects of Pueraria thomsonii flower extract (PFE) on obesity using obese Japanese males and females (BMI ≥ 25 kg/m(2)). Eighty-one obese subjects were randomly divided into three groups and consumed test food containing 300 mg of PFE, 200 mg of PFE, and a placebo over 12 weeks. The results indicate that PFE intake reduces BMI and decreases, the visceral fat area, but not the subcutaneous fat area. In addition, the decrease in visceral fat area showed no sexual dimorphism. Consequently, we propose that PFE intake expresses its BMI reduction effects via a decrease in visceral fat area.

  18. Effect of chemical factors on production of isoflavonoids in Pueraria tuberosa (Roxb.ex.Willd.) DC suspension culture.

    PubMed

    Goyal, Shaily; Ramawat, K G

    2007-12-01

    Suspension cultures of Pueraria tuberosa, a woody legume, have been established and using different concentrations of growth regulators, sucrose, ammonium and nitrate nitrogen, attempts have been made to improve their isoflavonoid content. The cell cultures grew well on all the treatments. Up to approximately 8 folds increased isoflavonoids content was recorded in the cultures grown in MS medium modified with nitrogen and supplemented with 1 mg 1(-1) of kinetin.

  19. Molecularly imprinted layer-coated monodisperse spherical silica microparticles toward affinity-enrichment of isoflavonoid glycosides from Radix puerariae.

    PubMed

    Luo, Jing; Zhang, Liying; Chen, Danjun; Wang, Peng; Zhao, Jiawei; Peng, Yan; Du, Shuhu; Zhang, Zhongping

    2012-06-21

    This paper reports the preparation of puerarin (PR) imprinted layer-coated silica microparticles toward selective recognition of PR and fast affinity-enrichment of the main isoflavonoid glycosides from the crude extract of Radix puerariae. Before the preparation, quantum mechanics (QM) method was applied to identify three kinds of common functional monomers capable of interaction with PR and then predicted optimal functional monomer (acrylamide, AA) and the relative molar ratio of template to functional monomer (PR/AA, 1:4). The obtained PR-imprinted silica microparticles were evaluated by transmission electron microscope (TEM) and rebinding experiments, exhibiting good morphology and high binding affinity to PR. Meanwhile, the rebinding amount of the imprinted microparticles to PR was nearly 2.1-folds that of non-imprinted microparticles. When the PR-imprinted microspheres were used as packing materials for solid-phase extraction, the recovery yields of PR, daidzin (DD) and genistin (GS) were simultaneously up to 90% by one-step extraction from the crude extract of Radix puerariae. Additionally, the PR-imprinted microparticles could be re-used for at least 5 times without losing any extraction efficiency. These results indicate that the PR-imprinted microparticles have highly selective adsorption capabilities to PR, DD and GS from the crude extract of Radix puerariae. The method of molecularly imprinted polymers (MIPs) coupled with solid-phase extraction (SPE) provides a good solution of the enrichment and separation of active extracts from complicated traditional Chinese medicine (TCM) with certain structures.

  20. Blackfeet Community Water Treatment Plant NPDES Permit

    EPA Pesticide Factsheets

    Under NPDES permit MT-0030643, the Blackfeet Tribe is authorized to discharge from its Blackfoot Community Water Treatment Plant in Glacier County, Montana, to an unnamed intermittent stream which flows to Two Medicine River.

  1. 75 FR 53687 - Southern Montana Electric Generation & Transmission Cooperative, Inc. v. NorthWestern Corporation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-01

    ...] Southern Montana Electric Generation & Transmission Cooperative, Inc. v. NorthWestern Corporation; Notice...) Rules of Practice and Procedure, 18 CFR 385.206, Southern Montana Electric Generation &...

  2. 76 FR 53880 - Southwest Montana Resource Advisory Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-30

    ... at 420 Barrett Street, Dillon, Montana (MT 59725). FOR FURTHER INFORMATION CONTACT: Patty Bates, Committee Coordinator, Beaverhead-Deerlodge National Forest, 420 Barrett Street, Dillon, MT 59725, (406)...

  3. Tickborne Relapsing Fever, Bitterroot Valley, Montana, USA

    PubMed Central

    Christensen, Joshua; Fischer, Robert J.; McCoy, Brandi N.; Raffel, Sandra J.

    2015-01-01

    In July 2013, a resident of the Bitterroot Valley in western Montana, USA, contracted tickborne relapsing fever caused by an infection with the spirochete Borrelia hermsii. The patient’s travel history and activities before onset of illness indicated a possible exposure on his residential property on the eastern side of the valley. An onsite investigation of the potential exposure site found the vector, Ornithodoros hermsi ticks, and 1 chipmunk infected with spirochetes, which on the basis of multilocus sequence typing were identical to the spirochete isolated from the patient. Field studies in other locations found additional serologic evidence and an infected tick that demonstrated a wider distribution of spirochetes circulating among the small mammal populations. Our study demonstrates that this area of Montana represents a previously unrecognized focus of relapsing fever and poses a risk for persons of acquiring this tickborne disease. PMID:25625502

  4. Seismic reflection study of Flathead Lake, Montana

    USGS Publications Warehouse

    Wold, Richard J.

    1982-01-01

    A seismic reflection survey of Flathead Lake, Montana, was carried out in 1970 to study the geologic structure underlying the lake. Approximately 200 km of track lines were surveyed resulting in about 140 km of useable data (Fig. 1). A one cu. in. air gun was used as the energy source. Navigation was by a series of theodolite sitings of the boat from pairs of shore-based control points. 

  5. WEST PIONEER WILDERNESS STUDY AREA, MONTANA.

    USGS Publications Warehouse

    Berger, Byron R.; Benham, John R.

    1984-01-01

    The West Pioneer Wilderness Study Area is in the Pioneer Mountains, Beaverhead County, Montana. A mineral-resource study of the area identified eight areas with molybdenum potential, four areas with gold-silver potential, one area with tungsten potential, and one area with barite potential. Several small mines were encountered, but none were accessible for the purposes of resource evaluation. No energy resources were identified in the study.

  6. A dolichopodid hotspot: Montana's Milligan Creek Canyon

    Treesearch

    Justin B. Runyon

    2016-01-01

    In southwest Montana, near the town of Three Forks, Milligan Creek cuts a small and seemingly mundane notch through dry limestone hills. Milligan Creek is unassuming and small enough to be effortlessly stepped over in most places. In fact, it flows underground for much of its 4-5 mile journey to the Jefferson River. Incredibly, forty-nine species of long-legged flies (...

  7. Wolf Point Substation, Roosevelt County, Montana

    SciTech Connect

    Not Available

    1991-05-01

    The Western Area Power Administration (Western), an agency of the United States Department of Energy, is proposing to construct the 115-kV Wolf Point Substation near Wolf Point in Roosevelt County, Montana (Figure 1). As part of the construction project, Western's existing Wolf Point Substation would be taken out of service. The existing 115-kV Wolf Point Substation is located approximately 3 miles west of Wolf Point, Montana (Figure 2). The substation was constructed in 1949. The existing Wolf Point Substation serves as a Switching Station'' for the 115-kV transmission in the region. The need for substation improvements is based on operational and reliability issues. For this environmental assessment (EA), the environmental review of the proposed project took into account the removal of the old Wolf Point Substation, rerouting of the five Western lines and four lines from the Cooperatives and Montana-Dakota Utilities Company, and the new road into the proposed substation. Reference to the new proposed Wolf Point Substation in the EA includes these facilities as well as the old substation site. The environmental review looked at the impacts to all resource areas in the Wolf Point area. 7 refs., 6 figs.

  8. Applicability of ERTS-1 to Montana geology

    NASA Technical Reports Server (NTRS)

    Weidman, R. M. (Principal Investigator); Alt, D. D.; Berg, R. A.; Johns, W. M.; Flood, R. E.; Hawley, K. T.; Wackwitz, L. K.

    1973-01-01

    The author has identified the following significant results. A detailed band 7 ERTS-1 lineament map covering western Montana and northern Idaho has been prepared and is being evaluated by direct comparison with geologic maps, by statistical plots of lineaments and known faults, and by field checking. Lineament patterns apparent in the Idaho and Boulder batholiths do not correspond to any known geologic structures. A band 5 mosaic of Montana and adjacent areas has been laid and a lineament annotation prepared for comparison with the band 7 map. All work to date indicates that ERTS-1 imagery is very useful for revealing patterns of high-angle faults, though much less useful for mapping rock units and patterns of low-angle faults. Large-scale mosaics of U-2 photographs of three test sites have been prepared for annotation and comparison with ERTS-1 maps. Mapping of Quaternary deposits in the Glacial Lake Missoula basin using U-2 color infrared transparencies has been successful resulting in the discovery of some deposits not previously mapped. Detailed work has been done for Test Site 354 D using ERTS-1 imagery; criteria for recognition of several rock types have been found. Photogeologic mapping for southeastern Montana suggest Wasatch deposits where none shown of geologic map.

  9. Montana Schools of Promise: Addressing Equity in American Indian Education

    ERIC Educational Resources Information Center

    Fishbaugh, Mary Susan E.; Dugi, Rosemarie; Schmitz, Stevie

    2016-01-01

    The American Indian presence in Montana enriches the state's culture. Educationally, however, there are gross disparities between academic performance of American Indian students when compared with the student population as a whole and with various ethnic/cultural subgroups. Montana's educational data mirror the Bureau of Indian Education national…

  10. Unravelling the Patchwork: Procedures Manual for Rural Montana Schools.

    ERIC Educational Resources Information Center

    Moore, Susan; And Others

    Designed to assist rural Montana schools in establishing and maintaining library-media centers, this procedures manual presents basic and specific information on all phases of operating a small school library. The first three chapters set forth the major responsibilities of the school librarian, Montana standards for school library personnel, and…

  11. 2015 Graduation and Dropout Report. Graduation Matters Montana

    ERIC Educational Resources Information Center

    Montana Office of Public Instruction, 2016

    2016-01-01

    In today's economy, a quality public education is the key to economic prosperity for young people. When Superintendent of Public Instruction Denise Juneau took office in 2009, there were 2,272 public school students who dropped out of school, and Montana had a dropout rate of five percent. For a state with a school population as small as Montana,…

  12. 2011 Montana Youth Risk Behavior Survey: Students with Disabilities

    ERIC Educational Resources Information Center

    Montana Office of Public Instruction, 2011

    2011-01-01

    This report presents the 2011 Montana Youth Risk Behavior Survey high school student frequency distributions for students with disabilities. These frequency distributions are based upon surveys with 1,672 high school students with disabilities in Montana during February of 2011. Frequency distributions may not total 1,672 due to nonresponse and…

  13. Indian Education for All: Essential Understandings Regarding Montana Indians. Revised

    ERIC Educational Resources Information Center

    Montana Office of Public Instruction, 2010

    2010-01-01

    In the year 1999, OPI [Montana Office of Public Instruction] brought together representatives from all the tribes in Montana and created 7 Essential Understandings. These are some of the major issues all tribes have in common. They form the basis for all of our curriculum efforts and initiatives. There is great diversity among the 12 tribal…

  14. 2011 Montana Youth Risk Behavior Survey: Alternative Schools

    ERIC Educational Resources Information Center

    Montana Office of Public Instruction, 2011

    2011-01-01

    This report presents the 2011 Montana Youth Risk Behavior alternative school student frequency distributions. These frequency distributions are based upon surveys with 274 alternative school students in Montana during February of 2011. Frequency distributions may not total 274 due to nonresponse and percents may not total 100 percent due to…

  15. 76 FR 22364 - Central Montana Resource Advisory Committee; Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service Central Montana Resource Advisory Committee; Correction AGENCY: Forest Service, USDA... Register of April 13, 2011, concerning a notice of meeting for the Central Montana Resource Advisory...

  16. 77 FR 54556 - Central Montana Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-05

    ...-54557] [FR Doc No: 2012-21809] DEPARTMENT OF AGRICULTURE Forest Service Central Montana Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Central Montana..., 109 Central Ave., Stanford, MT. VTC/Telephone will be available. Written comments may be submitted as...

  17. Resources for Teaching HERO: Food Service Occupations in Montana.

    ERIC Educational Resources Information Center

    Parsons, Angelina O.; Harris, Pamela R.

    This resource guide is designed to help home economics teachers in Montana to develop occupational programs for food service. It provides resources that can be used with the "Food Service Occupations in Montana: Scope and Sequence in Wage-Earning Home Economics." The guide contains 13 sections. The first section explains the core…

  18. Montana Schools of Promise: Addressing Equity in American Indian Education

    ERIC Educational Resources Information Center

    Fishbaugh, Mary Susan E.; Dugi, Rosemarie; Schmitz, Stevie

    2016-01-01

    The American Indian presence in Montana enriches the state's culture. Educationally, however, there are gross disparities between academic performance of American Indian students when compared with the student population as a whole and with various ethnic/cultural subgroups. Montana's educational data mirror the Bureau of Indian Education national…

  19. Montana's forest products industry and timber harvest, 2009

    Treesearch

    Chelsea P. McIver; Colin B. Sorenson; Charles E. Keegan; Todd A. Morgan; Jim Menlove

    2013-01-01

    This report traces the flow of Montana’s 2009 timber harvest through the primary wood-using industries; provides a description of the structure, capacity, and condition of Montana’s primary forest products industry; and quantifies volumes and uses of wood fiber. Historical wood products industry changes are discussed, as well as changes in harvest, production,...

  20. Montana's forest products industry and timber harvest, 2004

    Treesearch

    Timothy P. Spoelma; Todd A. Morgan; Thale Dillon; Alfred L. Chase; Charles E. Keegan; Larry T. DeBlander

    2008-01-01

    This report traces the flow of Montana's 2004 timber harvest through the primary wood-using industries; provides a description of the structure, capacity, and condition of Montana's primary forest products industry; and quantifies volumes and uses of wood fiber. Historical wood products industry changes are discussed, as well as changes in harvest, production...

  1. 76 FR 43259 - Southern Montana Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ... in Billings, Montana. The committee is meeting as authorized under the Secure Rural Schools and... Grad Montana Hotel and Convention Center, 5500 Midland Road, Billings, MT. Written comments should be sent to Babete Anderson, Custer National Forest, 1310 Main Street, Billings, MT 59105. Comments...

  2. Successful Strategies for Providing Online Credit Recovery in Montana

    ERIC Educational Resources Information Center

    Frazelle, Sarah

    2016-01-01

    This report examines common strategies used by six Montana schools that had high student passing rates in online credit recovery courses offered by the Montana Digital Academy (MTDA) in the 2013/14 school year. The study is based on analysis of interviews conducted with school-based facilitators who oversee the implementation of the online MTDA…

  3. 78 FR 20354 - Notice of Public Meeting; Central Montana Resource Advisory Council

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-04

    ... Bureau of Land Management Notice of Public Meeting; Central Montana Resource Advisory Council AGENCY... of the Interior, Bureau of Land Management (BLM) Central Montana Resource Advisory Council (RAC) will..., Montana. FOR FURTHER INFORMATION CONTACT: Gary L. ``Stan'' Benes, Central Montana District...

  4. 77 FR 75445 - Notice of Public Meeting; Central Montana Resource Advisory Council

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-20

    ... Bureau of Land Management Notice of Public Meeting; Central Montana Resource Advisory Council AGENCY.... Department of the Interior, Bureau of Land Management (BLM) Central Montana Resource Advisory Council (RAC... Main, Lewistown, Montana. FOR FURTHER INFORMATION CONTACT: Gary L. ``Stan'' Benes, Central Montana...

  5. 75 FR 57287 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-20

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin..., Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009. SUPPLEMENTARY INFORMATION:...

  6. 77 FR 13621 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-07

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin..., Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009, Marvin_Montoya@blm.gov ....

  7. 76 FR 76178 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-06

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT... Southgate Drive, Billings, Montana 59101-4669, telephone (701) 227-7730 or (406) 896-5009,...

  8. 77 FR 62527 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-15

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT... Drive, Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009,...

  9. 76 FR 41821 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-15

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT... Drive, Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009,...

  10. 76 FR 53695 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-29

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT... Drive, Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009,...

  11. 76 FR 29006 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-19

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin..., Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009. Persons who use...

  12. 78 FR 64531 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-29

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT... Southgate Drive, Billings, Montana 59101-4669, telephone (406) 896-5128 or (406) 896-5009,...

  13. 77 FR 64125 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-18

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT... Drive, Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009,...

  14. 77 FR 38321 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-27

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin..., Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009, Marvin_Montoya@blm.gov ....

  15. 77 FR 34402 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-11

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin..., Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009, Marvin_Montoya@blm.gov ....

  16. 77 FR 38320 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-27

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin..., Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009,...

  17. 76 FR 70163 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-10

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT... Drive, Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009,...

  18. 78 FR 66379 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-05

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT..., 5001 Southgate Drive, Billings, Montana 59101-4669, telephone (406) 896-5123 or (406)...

  19. 77 FR 34401 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-11

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin..., Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009, Marvin_Montoya@blm.gov ....

  20. 77 FR 13620 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-07

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Blaise..., Billings, Montana 59101-4669, telephone (406) 896-5128 or (406) 896-5009, bloderme@blm.gov . Persons...

  1. 77 FR 12075 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Blaise..., Billings, Montana 59101-4669, telephone (406) 896-5128 or (406) 896-5009, bloderme@blm.gov . Persons...

  2. 77 FR 31033 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-24

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin..., Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009, Marvin_Montoya@blm.gov ....

  3. 77 FR 34402 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-11

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Blaise..., Billings, Montana 59101-4669, telephone (406) 896-5128 or (406) 896-5009, bloderme@blm.gov . Persons...

  4. 76 FR 44946 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-27

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT... Drive, Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009,...

  5. 76 FR 17443 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-29

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin..., Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009. SUPPLEMENTARY INFORMATION:...

  6. 77 FR 34063 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-08

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Thomas..., Billings, Montana 59101-4669, telephone (406) 896-5125 or (406) 896-5009, tlaakso@blm.gov . Persons who...

  7. 77 FR 12075 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin..., Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009, Marvin_Montoya@blm.gov ....

  8. 75 FR 53334 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-31

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Survey, Bureau of Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669, telephone (406... Bureau of Indian Affairs, Rocky Mountain Region, Billings, Montana, and was necessary to...

  9. Mangiferin from Pueraria tuberosa reduces inflammation via inactivation of NLRP3 inflammasome

    PubMed Central

    Bulugonda, Ramakrishna K.; kumar, Kotha Anil; Gangappa, D.; Beeda, Harshavardhan; Philip, Gundala Harold; Muralidhara Rao, Dowlathabad; Faisal, Syed M.

    2017-01-01

    Recent reports have demonstrated the role of phyto-constituents in modulating inflammatory responses. Mangiferin isolated from Mangifera indica is known to induce potent anti-oxidative, anti-diabetic and anti-inflammatory activity. However, the molecular mechanism of its anti-inflammatory activity is not properly understood. In this study we have isolated Mangiferin from the tubers of Pueraria tuberosa (PT-Mangiferin) and analysed the mechanism of its potent anti-inflammatory effects in LPS stimulated RAW 264.7 mouse macrophage cell line and in a carrageenan induced air pouch model. PT-Mangiferin was non-toxic to primary cells but showed significant toxicity and apoptotic effect on cancerous cells. It significantly reduced the production of pro-inflammatory mediators (COX-2, iNOS and TNF-α) in LPS stimulated RAW 264.7 cells. Further, it has also reduced the generation of ROS and inhibited LPS induced NF-kB translocation in these cells. Additionally, PT-Mangiferin significantly reduced inflammation in a mouse air pouch model by inhibiting the infiltration of monocytes and neutrophils and reducing the production of cytokines. These effects were mediated via inactivation of NLRP3 inflammasome complex and its downstream signalling molecules. Taken together these results suggest that PT-Mangiferin is potent anti-inflammatory compound that reduces inflammation and holds promise in development of herbal based anti-inflammatory therapeutics in future. PMID:28218280

  10. Life-threatening interaction between the root extract of Pueraria lobata and methotrexate in rats

    SciTech Connect

    Chiang, H.-M.; Fang, S.-H.; Wen, K.-C.; Hsiu, S.-L.; Tsai, Shang-Yuan; Hou, Y.-C.; Chi, Y.-C.; Lee Chao, Pei-Dawn . E-mail: pdlee@mail.cmu.edu.tw

    2005-12-15

    Isoflavone supplements are nowadays widely used as alternative for hormone replacement therapy. However, the safety remains unanswered. This study attempted to investigate the effect of Pueraria lobata root decoction (PLRD), an isoflavone-rich herb, on the pharmacokinetics of methotrexate (MTX), a bicarboxylate antimetabolite with narrow therapeutic window. Rats were orally and intravenously given methotrexate alone and coadministered with PLRD. Blood samples were withdrawn via cardiopuncture at specific time points after drug administration. Serum methotrexate concentrations were assayed by specific monoclonal fluorescence polarization immunoassay method. Pharmacokinetic parameters were calculated using noncompartment model of WINNONLIN for both oral and intravenous data of MTX. Our results showed that coadministration of 4.0 g/kg and 2.0 g/kg of PLRD significantly increased the AUC{sub 0-t} by 207.8% and 127.9%, prolonged the mean residence time (MRT) by 237.8 and 155.2%, respectively, finally resulted in surprisingly high mortalities of 57.1% and 14.3% in rats. When MTX was given intravenously, the coadministration of PLRD at 4.0 g/kg significantly increased the half-life by 53.9% and decreased the clearance by 47.9%. In conclusion, the coadministration of PLRD significantly decreased the elimination and resulted in markedly increased exposure of MTX in rats.

  11. Preventive effect of Pueraria mirifica on testosterone-induced prostatic hyperplasia in Sprague Dawley rats.

    PubMed

    Masrudin, S S; Mohamad, J

    2015-12-01

    Pueraria mirifica (PM) extract contains phytoestrogen daidzein and genistein. In this study, we investigated the protective effect of PM extract, daidzein and genistein on a testosterone-induced prostatic hyperplasia in rats. Testosterone was administered at 3 mg kg(-1) to rats followed by the PM extract, daidzein and genistein for a period of 30 days with finasteride as positive control. The testosterone level was increased, indicating inhibition of 5α-reductase converting testosterone to dihydrotestosterone. This was confirmed by prostate-specific antigen level that significantly decreased when treated with PM extract, daidzein and genistein. The PM extract, daidzein and genistein reduced the increase in the prostate/body weight ratio in testosterone-induced rats. This gives indication that PM extract, daidzein and genistein possessed protective activity for the treatment of benign prostatic hyperplasia. The analysis of histoarchitechture of the prostate has also shown that there was a significant improvement in prostatic cells of the testosterone-induced rats when treated with PM extract, daidzein and genistein.

  12. Genetic variation in Pueraria lobata (Fabaceae), an introduced, clonal, invasive plant of the southeastern United States.

    PubMed

    Pappert, R A; Hamrick, J L; Donovan, L A

    2000-09-01

    Pueraria lobata (kudzu), a clonal, leguminous vine, is invading the southeastern United States at a rate of 50 000 ha per year. Genetic variability and clonal diversity were measured in 20 southeastern U.S. populations using 14 allozyme loci. Within its U.S. range, 92.9% of the loci were polymorphic and overall genetic diversity was 0.290. Such high levels of genetic diversity are consistent with its history of multiple introductions over an extended period of time. The average proportions of polymorphic loci and genetic diversity within populations were 55.7% (range = 28.6-85.7%) and 0.213 (range = 0.114-0.317), respectively. The proportion of total genetic diversity found among populations was similar to species with equivalent life history characters (G(ST) = 0.199). No regional patterns of variation were seen. The number of putative genotypes in each population ranged from 2 to 26. Mean genotypic diversity was 0.694, ranging from 0.223 to 0.955. Such high levels of genotypic diversity indicate that local sites are often colonized by several propagules (most likely seeds) and/or that sexual reproduction occurs within populations after establishment. An excess of heterozygosity was observed in populations with few unique genets, implying that selection for highly heterozygous individuals may occur in populations of P. lobata.

  13. Phenotypic plasticity of Neonotonia wightii and Pueraria phaseoloides grown under different light intensities.

    PubMed

    Santos, Leonardo D T; Da Cruz, Leandro R; Dos Santos, Samuel A; Sant'anna-Santos, Bruno F; Dos Santos, Izabela T; De Oliveira, Ariane M; Barros, Rodrigo E; Santos, Márcia V; Faria, Rodrigo M

    2015-03-01

    Plants have the ability to undergo morphophysiological changes based on availability of light. The present study evaluated biomass accumulation, leaf morphoanatomy and physiology of Neonotonia wightii and Pueraria phaseoloides grown in full sunlight, as well as in 30% and 50% shade. Two assays were performed, one for each species, using a randomized block design with 10 replicates. A higher accumulation of fresh mass in the shoot of the plants was observed for both species under cultivation in 50% shade, while no differences were detected between the full sunlight and 30% shade. N. wightii and P. phaseoloides showed increase in area and reduction in thickness leaf when cultivated in 50% shade. There were no changes in photosynthetic rate, stomatal conductance, water use efficiency and evapotranspiration of P. phaseoloides plants because growth environment. However, the shade treatments caused alterations in physiological parameters of N. wightii. In both species, structural changes in the mesophyll occurred depending on the availability of light; however, the amount of leaf blade tissue remained unaltered. Despite the influence of light intensity variation on the morphophysiological plasticity of N. wightii and P. phaseoloides, no effects on biomass accumulation were observed in response to light.

  14. Evaluation of Anti-Inflammatory Properties of Isoorientin Isolated from Tubers of Pueraria tuberosa

    PubMed Central

    Anilkumar, Kotha; Reddy, Gorla V.; Azad, Rajaram; Yarla, Nagendra Sastry; Dharmapuri, Gangappa; Srivastava, Anand

    2017-01-01

    Inflammation is the major causative factor of different diseases such as cardiovascular disease, diabetes, obesity, osteoporosis, rheumatoid arthritis, inflammatory bowel disease, and cancer. Anti-inflammatory drugs are often the first step of treatment in many of these diseases. The present study is aimed at evaluating the anti-inflammatory properties of isoorientin, a selective cyclooxygenase-2 (COX-2) inhibitor isolated from the tubers of Pueraria tuberosa, in vitro on mouse macrophage cell line (RAW 264.7) and in vivo on mouse paw edema and air pouch models of inflammation. Isoorientin reduced inflammation in RAW 264.7 cell line in vitro and carrageenan induced inflammatory animal model systems in vivo. Cellular infiltration into pouch tissue was reduced in isoorientin treated mice compared to carrageenan treated mice. Isoorientin treated RAW 264.7 cells and animals showed reduced expression of inflammatory proteins like COX-2, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), 5-lipoxygenase (5-LOX), and interleukin 1-β (IL-1-β) both in vitro and in vivo. The antioxidant enzyme levels of catalase and GST were markedly increased in isoorientin treated mice compared to carrageenan treated mice. These results suggest that isoorientin, a selective inhibitor of COX-2, not only exerts anti-inflammatory effects in LPS induced RAW cells and carrageenan induced inflammatory model systems but also exhibits potent antioxidant properties. PMID:28243356

  15. Pueraria tuberosa DC Extract Improves Androgenesis and Sexual Behavior via FSH LH Cascade

    PubMed Central

    Chauhan, Nagendra Singh; Sharma, Vikas; Christine Helena Frankland Sawaya, Alexandra; Dixit, V. K.

    2013-01-01

    The aim of this study was to investigate the effects of ethanolic extract of Pueraria tuberosa (PT) on sexual behaviour and androgenic activity. Male albino rats were divided into four groups of six animals each: control group 1 (2% acacia solution), PT-treated group 2 (50 mg/Kg), PT-treated group 3 (100 mg/Kg), and PT-treated group 4 (150 mg/Kg). Sexual behavior of male rats in the presence of a female rat was recorded. The treated groups were evaluated for sexual parameters. The extract was characterized using LC-MS. The effect of treatment on anabolic and weight of secondary sexual organs was determined. The histological changes in section of testis and epididymis after treatment were observed. Sperm count in epididymis and fructose content in seminal vesicles were also measured. Levels of hormones like FSH, LH, and T were determined. A dose-dependent increase in sexual behaviors was evidenced in the animals of extract treated groups. Increase in testis weight was recorded in PT. At the highest dose PT also affects the hormones level. The four compounds namely puerarin, daidzein, biochanin-A and formononetin were identified in ethanolic extract using LC-MS. It concluded that PT extract possesses androgenic effect and it significantly increased the sexual behaviour and hormones level. PMID:24489512

  16. Constituents with potent α-glucosidase inhibitory activity from Pueraria lobata (Willd.) ohwi.

    PubMed

    Wang, Xiao-Ling; Jiao, Fu-Rong; Yu, Meng; Lin, Li-Bin; Xiao, Jian; Zhang, Qiang; Wang, Le; Duan, Dong-Zhu; Xie, Gang

    2017-05-01

    One new flavone hydrate named lobatflavate (1), one new chromone named lobatchrosin (2), and one new isoflavone named 3S,4R-tuberosin (3), along with four known isoflavone analogues (4-7), were isolated from the traditional Chinese medicinal plant of Pueraria lobata (Willd.) ohwi. Their structures were elucidated by extensive spectroscopic methods of IR, UV, HR-ESI-MS, 1D and 2D NMR. The absolute configuration of 3 was determined by CD spectrum associated with TD-DFT calculation analysis. All compounds except for 2 were assayed the inhibitory activity against α-glucosidase. Every tested compound was proved to be more active than positive control of acarbose. Of which 1 and 4 showed significant activity with IC50 value of 1.79μM and 23.01μM (IC50 of acarbose was 1998.79μM). Enzyme kinetic experiments revealed that 1 was irreversible whereas 4 was reversible and non-competitive α-glucosidase inhibitors. Moreover, structure-activity relationship was discussed and the docking studies of 1, 3 and 4 were also carried out. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Pueraria mirifica, a phytoestrogen-rich herb, prevents bone loss in orchidectomized rats.

    PubMed

    Urasopon, Nontakorn; Hamada, Yuzuru; Asaoka, Kazuo; Cherdshewasart, Wichai; Malaivijitnond, Suchinda

    2007-03-20

    Estrogens and estrogen-like substances have been reported to play an important role in male bone homeostasis and to prevent bone loss. Pueraria mirifica (Leguminosae), a Thai herbal plant, containing a high amount of phytoestrogens was a choice of interest for this study. We examined the effects of crude P. mirifica on bone loss and influences on reproductive organs in male rats. Using fully mature and orchidectomized (ORX) rats, the effects of 0, 10, 100 and 1000 mg/kgB.W./day of P. mirifica and 0.1mg/kg B.W./day of 17 alpha-ethinylestradiol (a positive control) were evaluated on bone mineral density (BMD) and bone mineral content (BMC) measured with a peripheral Quantitative Computerized Tomography (pQCT) densitometry. Bone loss in trabecular and cortical bones of the various sites of axial bone (fourth lumbar vertebral body) and long bones (tibia and femur) after ORX was dose-dependently prevented by P. mirifica. The effects were specific on bone types and sites. The weights of the accessory sex organs, seminal vesicle and ventral prostrate gland, which significantly decreased after 3-month of ORX, were not altered by P. mirifica. The results suggest that P. mirifica treatment may be useful to prevent an osteoporosis in elderly hypogonadism subjects without influences on reproductive organs.

  18. Protective effect of Pueraria tuberosa DC. embedded biscuit on cisplatin-induced nephrotoxicity in mice.

    PubMed

    Tripathi, Yamini B; Nagwani, Santosh; Mishra, Pooja; Jha, Alok; Rai, Shashi Pandey

    2012-01-01

    Recently, the nephroprotective property of Pueraria tuberosa DC. tuber (PT) has been reported by our group. Here, PT-embedded biscuits were prepared and tested on cisplatin-induced nephrotoxicity in Swiss albino mice. The PT powder was characterized by RAPD (random amplified polymorphic DNA) to ascertain its authenticity and PT biscuits were prepared in different concentrations (1, 2, or 4 g of PT powder). These biscuits were given as diet for a total of 10 days, but on the 7th day cisplatin injection (8 mg/kg bw, i.p.) was given. On the 10th day animals were killed to collect kidneys for assessment of antioxidant status. Blood samples were collected on both the 7th and 10th days for assessment of liver and kidney functions. In mice, PT biscuit showed significant protection against cisplatin-induced nephrotoxicity, but there was a transient rise in alanine aminotransferase and aspartate aminotransferase at the dose of 4 g PT biscuit. Therefore, it is suggested that PT biscuit might be an effective food supplement for cancer patients undergoing cisplatin-chemotherapy. However, periodical liver function monitoring is required, especially when PT is used for longer periods or at higher doses.

  19. [Determination of five components in Pueraria labta decoction with reference extraction method].

    PubMed

    Xue, Cuijuan; Zhang, Jun; Jing, Wenguang; Wang, Yuesheng; Si, Nan; Liu, An

    2012-08-01

    To establish the reference extraction method for determining five components contained in Pueraria labta decoction and detecting the applicability and feasibility of the application of reference extracts according to the quality standard of traditional Chinese medicine. Zorbax C18 chromatographic column (4.6 mm x 250 mm, 5 microm) was used, with methanol-0.02% phosphoric acid (25:75) as the mobile phase and the detection wavelength was set at 250 nm. Self-prepared reference extracts of P. labta was used for determining the content of 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, isoflavoues aglycone-8-C-apiose-(1--> 6) glucoside and daidzin and compare with the experimental results obtained with the single reference substance determination method. The reference extraction determination method showed high precision, stability and reproducibility, with coincident determination results with the results obtained with the single reference substance determination method. The reference extraction determination method of P. labta can be used for quality control of P. labta herbs. The study preliminarily proves the feasibility of the application of P. labta in traditional Chinese medicine.

  20. Physical Stability and HPLC Analysis of Indian Kudzu (Pueraria tuberosa Linn.) Fortified Milk

    PubMed Central

    Rastogi, Subha; Katara, Antariksha; Pandey, Madan M.; Arora, Sumit; Singh, R. R. B.; Rawat, A. K. S.

    2013-01-01

    Functional foods provide health benefit beyond basic nutrition. Functional foods fortified with plant ingredients are well known. Ayurveda (Indian System of Medicine) has found several ways in which the medicinal benefits of herbs can be conveyed via certain foods as carriers. Milk is one such carrier which has been effectively used to deliver phytochemicals for targeted health benefits. Indian Kudzu or Pueraria tuberosa Linn. (Fabaceae) is an important medicinal plant of Ayurveda, and experiments suggest that it enhances the health benefits of milk when taken with milk as a carrier. Different milk combinations with P. tuberosa were prepared by homogenizing pasteurized toned milk with its ethanolic and hot water extracts and their stability with reference to pH and coagulation was studied over a period of 15 days. The combinations were also analyzed for puerarin, the major isoflavone C-glucoside present in P. tuberosa, through high-performance liquid chromatography using photo diode array detector. It was observed that there was no precipitate formation and the pH also did not change during the study period indicating their physical stability under the experimental conditions. Also there was no significant change in the content of puerarin during the study period, thereby indicating the chemical stability of the samples. These studies will be useful for developing milk nutraceuticals fortified with Indian Kudzu which has the potential to be included as an ingredient in health and functional foods. PMID:23690842

  1. Pueraria tuberosa DC extract improves androgenesis and sexual behavior via FSH LH cascade.

    PubMed

    Chauhan, Nagendra Singh; Sharma, Vikas; Thakur, Mayank; Christine Helena Frankland Sawaya, Alexandra; Dixit, V K

    2013-01-01

    The aim of this study was to investigate the effects of ethanolic extract of Pueraria tuberosa (PT) on sexual behaviour and androgenic activity. Male albino rats were divided into four groups of six animals each: control group 1 (2% acacia solution), PT-treated group 2 (50 mg/Kg), PT-treated group 3 (100 mg/Kg), and PT-treated group 4 (150 mg/Kg). Sexual behavior of male rats in the presence of a female rat was recorded. The treated groups were evaluated for sexual parameters. The extract was characterized using LC-MS. The effect of treatment on anabolic and weight of secondary sexual organs was determined. The histological changes in section of testis and epididymis after treatment were observed. Sperm count in epididymis and fructose content in seminal vesicles were also measured. Levels of hormones like FSH, LH, and T were determined. A dose-dependent increase in sexual behaviors was evidenced in the animals of extract treated groups. Increase in testis weight was recorded in PT. At the highest dose PT also affects the hormones level. The four compounds namely puerarin, daidzein, biochanin-A and formononetin were identified in ethanolic extract using LC-MS. It concluded that PT extract possesses androgenic effect and it significantly increased the sexual behaviour and hormones level.

  2. Puerariae radix isoflavones and their metabolites inhibit growth and induce apoptosis in breast cancer cells

    SciTech Connect

    Lin, Y.-J.; Hou, Y.C.; Lin, C.-H.; Hsu, Y.-A.; Sheu, Jim J.C.; Lai, C.-H.; Chen, B.-H.; Lee Chao, Pei-Dawn; Wan Lei Tsai, F.-J.

    2009-01-23

    Puerariae radix (PR) is a popular natural herb and a traditional food in Asia, which has antithrombotic and anti-allergic properties and stimulates estrogenic activity. In the present study, we investigated the effects of the PR isoflavones puerarin, daidzein, and genistein on the growth of breast cancer cells. Our data revealed that after treatment with PR isoflavones, a dose-dependent inhibition of cell growth occurred in HS578T, MDA-MB-231, and MCF-7 cell lines. Results from cell cycle distribution and apoptosis assays revealed that PR isoflavones induced cell apoptosis through a caspase-3-dependent pathway and mediated cell cycle arrest in the G2/M phase. Furthermore, we observed that the serum metabolites of PR (daidzein sulfates/glucuronides) inhibited proliferation of the breast cancer cells at a 50% cell growth inhibition (GI{sub 50}) concentration of 2.35 {mu}M. These results indicate that the daidzein constituent of PR can be metabolized to daidzein sulfates or daidzein glucuronides that exhibit anticancer activities. The protein expression levels of the active forms of caspase-9 and Bax in breast cancer cells were significantly increased by treatment with PR metabolites. These metabolites also increased the protein expression levels of p53 and p21. We therefore suggest that PR may act as a chemopreventive and/or chemotherapeutic agent against breast cancer by reducing cell viability and inducing apoptosis.

  3. Identification of isoflavone glycosides in Pueraria lobata cultures by tandem mass spectrometry.

    PubMed

    Prasain, Jeevan K; Reppert, Adam; Jones, Kenneth; Moore, D Ray; Barnes, Stephen; Lila, Mary Ann

    2007-01-01

    Isoflavones in the methanolic extracts of kudzu (Pueraria lobata) callus, suspension and root cultures were compared in order to develop an experimental system in which puerarin (daidzein 8-C-glucoside) and other isoflavones could be synthesised in vitro. Quantitative variation of puerarin and other known isoflavones was estimated in kudzu culture extracts using HPLC-UV. The highest and lowest amounts of puerarin (14.56 and 0.33 mg/g) were present in in vitro root cultures and leaf tissue-derived callus cultures, respectively. A total of 48 isoflavone metabolites were detected in extracts of kudzu root cultures by HPLC-MS/MS, and the structures of 33 of them were tentatively assigned. Amongst these, 12 isoflavone C-glycosides were identified. Hydroxyderivatives of puerarin in several isomeric forms were detected, some of which have not been previously reported in kudzu root. The molecular weights, interpretation of characteristic fragment ions obtained from HPLC-MS/MS and comparison with reported data allowed the putative identification of the isoflavone metabolites.

  4. Characterization of kudzu (Pueraria spp.) resistance to Phakopsora pachyrhizi, the causal agent of soybean rust.

    PubMed

    Jordan, Stephen A; Mailhot, Daniel J; Gevens, Amanda J; Marois, Jim J; Wright, David L; Harmon, Carrie L; Harmon, Philip F

    2010-09-01

    Kudzu (Pueraria spp.) is an accessory host for soybean rust (SBR) (caused by Phakopsora pachyrhizi) that is widespread throughout the southeastern United States. An expanded survey of kudzu sites was conducted in 2008 to determine the proportion of natural resistance in the north-Florida kudzu population. Of the 139 sites evaluated, approximately 18% were found to be free of SBR infection, while 23% had reduced sporulation. Ten accessions of kudzu from north-central Florida were characterized for their response to challenge by a single isolate of P. pachyrhizi under laboratory conditions. Three outcomes were observed: tan lesions with profuse sporulation (susceptible); reddish-brown lesions with delayed, reduced sporulation (resistant); and an immune response in which no lesions developed (immune). Of the 10 accessions, 6 were susceptible, 3 were immune, and 1 was resistant. Cytological examination revealed that resistant interactions were typified by early onset of a multicell hypersensitive response (HR) while typical immune interactions were the result of cell wall depositions that blocked penetration in combination with early onset of the HR. Quantitative real-time polymerase chain reaction was performed to determine the extent of colonization. After 15 days, there was 10-fold less P. pachyrhizi DNA present in resistant compared with susceptible kudzu, while the amount of P. pachyrhizi DNA present in the immune kudzu was below the detection level. Susceptible kudzu had approximately half the amount of P. pachyrhizi DNA present when compared with a susceptible soybean cultivar.

  5. Physical Stability and HPLC Analysis of Indian Kudzu (Pueraria tuberosa Linn.) Fortified Milk.

    PubMed

    Rastogi, Subha; Katara, Antariksha; Pandey, Madan M; Arora, Sumit; Singh, R R B; Rawat, A K S

    2013-01-01

    Functional foods provide health benefit beyond basic nutrition. Functional foods fortified with plant ingredients are well known. Ayurveda (Indian System of Medicine) has found several ways in which the medicinal benefits of herbs can be conveyed via certain foods as carriers. Milk is one such carrier which has been effectively used to deliver phytochemicals for targeted health benefits. Indian Kudzu or Pueraria tuberosa Linn. (Fabaceae) is an important medicinal plant of Ayurveda, and experiments suggest that it enhances the health benefits of milk when taken with milk as a carrier. Different milk combinations with P. tuberosa were prepared by homogenizing pasteurized toned milk with its ethanolic and hot water extracts and their stability with reference to pH and coagulation was studied over a period of 15 days. The combinations were also analyzed for puerarin, the major isoflavone C-glucoside present in P. tuberosa, through high-performance liquid chromatography using photo diode array detector. It was observed that there was no precipitate formation and the pH also did not change during the study period indicating their physical stability under the experimental conditions. Also there was no significant change in the content of puerarin during the study period, thereby indicating the chemical stability of the samples. These studies will be useful for developing milk nutraceuticals fortified with Indian Kudzu which has the potential to be included as an ingredient in health and functional foods.

  6. Anti-Hyperglycemic Effect of a Kudzu (Pueraria lobata) Vine Extract in Ovariectomized Mice.

    PubMed

    Tanaka, Teruyoshi; Yokota, Yukihiro; Tang, Hanjun; Zaima, Nobuhiro; Moriyama, Tatsuya; Kawamura, Yukio

    2016-01-01

    Postmenopausal diabetes is exacerbated by estrogen deficiency. Ovariectomized (OVX) animal models can be used to develop strategies for preventing or treating postmenopausal symptoms. We previously found that a diet containing kudzu (Pueraria lobata) vine ethanol extract (PVEE) suppressed weight gain in OVX mice. Therefore, this study further elucidated how PVEE affected OVX mice. Ten-week-old OVX or sham-operated mice were fed diets containing either no PVEE (control) or 20 mg•kg(-1)•d(-1) PVEE for 8 wk, 5 mg•kg(-1)•d(-1) PVEE for 24 wk, or 20 mg•kg(-1)•d(-1) puerarin (daidzein-8-C-glucoside), a major isoflavone present in PVEE, for 10 wk. The effects of puerarin on glucose tolerance were also tested in OVX mice. The experimental diets were not associated with any abnormalities in any mice tested in the present study. Weight gain and serum glucose levels were increased in OVX mice and these effects were significantly attenuated in OVX mice that consumed PVEE (5 or 20 mg•kg(-1)•d(-1)) or puerarin. Puerarin-treated OVX mice also showed reduced serum glucose levels following administration of 1,000 mg•kg(-1) glucose. These results suggested that puerarin contributed to PVEE-mediated improvements in glucose metabolism in OVX mice. Although further studies are needed to clarify the molecular mechanism underlying these observations, PVEE and puerarin could provide effective approaches to the amelioration of postmenopausal diabetes.

  7. A Report on the Health of Montana's Infants. 1996 Montana Special Report No. 1.

    ERIC Educational Resources Information Center

    Healthy Mothers, Healthy Babies--The Montana Coalition, Helena.

    This brief Kids Count report reviews principal adverse birth outcomes that affect the status of infants in Montana, including infant mortality and low birth weight. Statistics and brief summaries are provided in the following areas: (1) infant mortality (on the decline since 1989); (2) low birth rate (remaining steady from 1988 through 1992); (3)…

  8. Secondary Metabolites, Glandular Trichomes and Biological Activity of Sideritis montana L. subsp. montana from Central Italy.

    PubMed

    Venditti, Alessandro; Bianco, Armandodoriano; Frezza, Claudio; Serafini, Mauro; Giacomello, Ginevra; Giuliani, Claudia; Bramucci, Massimo; Quassinti, Luana; Lupidi, Giulio; Lucarini, Domenico; Papa, Fabrizio; Maggi, Filippo

    2016-10-01

    Sideritis montana subsp. montana is a small annual herb occurring in countries bordering the Mediterranean and Balkan regions. The secondary metabolism of this plant has not been fully explored so far. The aim of the present study was to understand the complex mixture of secondary metabolites and the type of secretory structures. The polar constituents were isolated by column chromatography from the ethanolic extract, and their structure was elucidated by NMR and MS. The essential oil was isolated by hydrodistillation and analysed by GC/MS. The plant indumentum was studied by light and scanning electron microscopy. To complete the work, the essential oil antioxidant activity and cytotoxicity on tumor cells were evaluated by DPPH, ABTS, FRAP, and MTT methods. Four different classes of secondary metabolites were isolated, namely flavonoids, caffeoylquinic derivatives, glycosidic hydroquinones and iridoids. The essential oil was mainly characterized by sesquiterpenene hydrocarbons. Peltate and long-capitate hairs were the main sites where terpenes and polar constituents are produced. The secondary metabolites found in S. montana subsp. montana are of chemotaxonomic interest, some of them being typical of the genus Sideritis. The trichomes types observed partially differ from those described in other members of the genus Sideritis. The essential oil showed noteworthy inhibition on tumor cells. © 2016 Wiley-VHCA AG, Zürich.

  9. Resource Sharing in Montana: A Study of Interlibrary Loan and Alternatives for a Montana Union Catalog.

    ERIC Educational Resources Information Center

    Matthews, Joseph R.

    This study recommends a variety of actions to create and maintain a Montana union catalog (MONCAT) for more effective usage of in-state resources and library funds. Specifically, it advocates (1) merger of existing COM, machine readable bibliographic records, and OCLC tapes into a single microform catalog; (2) acceptance of only machine readable…

  10. Resource Sharing in Montana: A Study of Interlibrary Loan and Alternatives for a Montana Union Catalog.

    ERIC Educational Resources Information Center

    Matthews, Joseph R.

    This study recommends a variety of actions to create and maintain a Montana union catalog (MONCAT) for more effective usage of in-state resources and library funds. Specifically, it advocates (1) merger of existing COM, machine readable bibliographic records, and OCLC tapes into a single microform catalog; (2) acceptance of only machine readable…

  11. Role and Scope of the Montana University System. Adopted June 25, 1979 by the Board of Regents, Montana University System.

    ERIC Educational Resources Information Center

    Montana State Office of the Commissioner of Higher Education, Helena.

    The role and scope of the Montana University and postsecondary education system are described. It is explained that the primary goal of the Montana University System is to use as effectively as possible the resources available to it in providing high quality educational opportunities and service to the people of the state, encompassing the three…

  12. Simultaneous determination of seventeen mycotoxins residues in Puerariae lobatae radix by liquid chromatography-tandem mass spectrometry.

    PubMed

    Wang, Shufang; Cheng, Ling; Ji, Shen; Wang, Ke

    2014-09-01

    This work reported an efficient and accurate liquid chromatography tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of seventeen mycotoxins in Puerariae lobatae radix, a frequently used traditional Chinese medicine (TCM). The effects of four different clean-up methods, including TC-M160, TC-T220, Mycosep 227, and QuEChERS method, on the recoveries of mycotoxins were investigated and compared. Finally, TC-M160 was chosen for better recovery and repeatability for mycotoxins analysis. The analytes were separated on an Agilent ZORBAX SB C18 column (4.6mm×250mm, 5μm particle size), and eluted with a mobile phase consisting of (A) water containing 0.1% formic acid and (B) acetonitrile containing 0.1% formic acid at a flow rate of 0.6mL/min. The separated compounds were detected by a triple quadrupole mass spectrometer operating in positive electrospray ionization with multiple reaction monitoring (MRM) mode. The results of method validation accorded with the requirement of analytical method for mycotoxins in COMMISSION REGULATION (EC) No 401/2006. The developed method was successfully applied for determination of mycotoxins in seventeen batches of Puerariae lobatae radix collected from different provinces of China. Three batches of them were found with contamination of mycotoxins AFB1 at (0.751±0.176)μg/kg, T-2 at (1.10±0.01)μg/kg, and T-2 at (0.853±0.044)μg/kg, respectively. The results demonstrated that the proposed method was suitable for monitoring mycotoxins residues in Puerariae lobatae radix.

  13. Human Jamestown canyon virus infection --- Montana, 2009.

    PubMed

    2011-05-27

    Jamestown Canyon virus (JCV) is a mosquito-borne zoonotic pathogen belonging to the California serogroup of bunyaviruses. Although JCV is widely distributed throughout temperate North America, reports of human JCV infection in the United States are rare. This is the first report of human JCV infection detected in Montana, one of only 15 cases reported in the United States since 2004, when JCV became reportable. On May 26, 2009, a man aged 51 years with no travel history outside of Montana went to a local emergency department immediately following onset of fever, severe frontal headache, dizziness, left-sided numbness, and tingling. His blood pressure was elevated. Stroke was ruled out, oxygen was administered, medication was prescribed for hypertension, and the patient was sent home. One week later, the patient visited his primary-care physician complaining of continued neurologic symptoms consistent with acute febrile encephalitis and recent mosquito bites. Although West Nile virus (WNV) disease was diagnosed based on detection of WNV-immunoglobulin M (IgM) and G (IgG) antibodies, subsequent testing indicated that the WNV antibodies were from a past infection and that his illness was caused by JCV. The final diagnosis of JCV infection was based on positive JCV-specific IgM enzyme-linked immunosorbent assay (ELISA) results and a fourfold rise in paired sample JCV plaque reduction neutralization test (PRNT) titers. This finding represents a previously unrecognized risk for JCV infection in Montana; clinicians should consider JCV infection when assessing patients for suspected arboviral infections.

  14. ABSAROKA PRIMITIVE AREA AND VICINITY, MONTANA.

    USGS Publications Warehouse

    Wedow, Helmuth; Bannister, D'Arcy P.

    1984-01-01

    A mineral-resource appraisal of the Absaroka Primitive Area, Montana indicates a probable resource potential for copper-molybdenum and gold in parts of the area. An area favorable for the occurrence of early Tertiary porphyry-type copper-molybdenum and associated gold deposits lies northwest of Cooke City, along the Cooke City structural sag in the Beartooth uplift. An area favorable for stratabound gold deposits of the Homestake type is in the western part of the area, in and around the Jardine-Crevice Mountain mining district and in lower Hellroaring Creek valley. There is little promise for the occurrence of energy resources in the primitive area.

  15. SELWAY-BITTERROOT WILDERNESS, IDAHO AND MONTANA.

    USGS Publications Warehouse

    Toth, Margo I.; Zilka, Nicholas T.

    1984-01-01

    Mineral-resource studies of the Selway-Bitterroot Wilderness in Idaho County, Idaho, and Missoula and Ravalli Counties, Montana, were carried out. Four areas with probable and one small area of substantiated mineral-resource potential were recognized. The areas of the Running Creek, Painted Rocks, and Whistling Pig plutons of Tertiary age have probable resource potential for molybdenum, although detailed geochemical sampling and surface investigations failed to recognize mineralized systems at the surface. Randomly distributed breccia zones along a fault in the vicinity of the Cliff mine have a substantiated potential for small silver-copper-lead resources.

  16. CHARLES M. RUSSELL WILDLIFE REFUGE, MONTANA.

    USGS Publications Warehouse

    Rice, Dudley D.; Miller, Michael S.

    1984-01-01

    A mineral survey of the Charles M. Russell Wildlife Refuge in Montana indicates that parts of the area have demonstrated resources of low-rank coal and bentonite in areas of substantiated potential and all of the area is assigned a probable resource potential for oil and gas because it is underlain by sedimentary strata known to contain hydrocarbons in other areas. Potential hydrocarbon accumulations, including both oil and gas, are difficult to delineate because of the absence of subsurface control points within the refuge. Geophysical surveys and directional drilling along the fringes of the wildlife refuge would aid in refining resource estimates for organic fuels. 1 ref.

  17. Free-ranging Rocky Mountain bighorn sheep and an outbreak of inflammatory bowel disease along the Clark Fork River in Plains, Montana.

    PubMed

    Pierce, Ellen S

    2012-10-01

    Nine individuals with ulcerative colitis or Crohn disease grew up or lived in Plains, Montana, a 1,200-person community adjacent to the Clark Fork River near herds of free ranging Rocky Mountain bighorn sheep. This inflammatory bowel disease outbreak is similar to others that have occurred along rivers contaminated by animal feces.

  18. Effect of invader litter chemistries on soil organic matter compositions: consequences of Polygonum cuspidatum and Pueraria lobata invasions

    NASA Astrophysics Data System (ADS)

    Tharayil, N.; Tamura, M.

    2012-12-01

    Carbon fixation during photosynthesis forms the precursor of all organic carbon in soil and the predominant source of energy that drives soil microbial processes; hence the molecular identity of the fixed carbon could influence the formation of soil organic matter (SOM). Due to their high resource acquisition and resource use efficiencies, some invasive plants can input disproportionately high quantities of litter that are qualitatively distinctive, and this could influence the accrual of organic carbon and overall carbon cycling in invaded habitats. Hence, we hypothesized that invasive plants with unique litter chemistries would significantly influence the overall carbon cycling in the invaded soils. We tested this hypothesis by comparing plants exhibiting recalcitrant vs. labile litter chemistries using japanese knotweed (Polygonum cuspidatum) and kudzu (Pueraria lobata), respectively. Japanese knotweed produces low litter abundant in polyphenols which selectively hinders microbially mediated decomposition and re-synthesis; whereas kudzu produces low C:N, high quality litter that can stimulate microbial decomposition. Soil samples were collected at 5-cm intervals and from inside and outside 15 to 20 year old stands of the invasive species. The novelty of our study was that both of our study species were invading into soils of contrasting substrate qualities relative to the invading litter quality. The molecular composition of carbon in the soils and the degradation stage of the SOM were assessed with a biomarker approach using gas chromatography-mass spectrometry to determine the source of biomolecules (plant or microbes). Stability of SOM fractions was assessed through oxidation with hydrogen peroxide, serving as a proxy of biological degradation, followed by stable isotope analysis. Fungal communities dominated the uppermost soils under knotweed whereas kudzu litter suppressed fungal biomass in the top 10-cm. In constrast, increase in active microbial biomass C

  19. The University of Montana's Blue Mountain Observatory

    NASA Astrophysics Data System (ADS)

    Friend, D. B.

    2004-12-01

    The University of Montana's Department of Physics and Astronomy runs the state of Montana's only professional astronomical observatory. The Observatory, located on nearby Blue Mountain, houses a 16 inch Boller and Chivens Cassegrain reflector (purchased in 1970), in an Ash dome. The Observatory sits just below the summit ridge, at an elevation of approximately 6300 feet. Our instrumentation includes an Op-Tec SSP-5A photoelectric photometer and an SBIG ST-9E CCD camera. We have the only undergraduate astronomy major in the state (technically a physics major with an astronomy option), so our Observatory is an important component of our students' education. Students have recently carried out observing projects on the photometry of variable stars and color photometry of open clusters and OB associations. In my poster I will show some of the data collected by students in their observing projects. The Observatory is also used for public open houses during the summer months, and these have become very popular: at times we have had 300 visitors in a single night.

  20. Prevalence of diagnosed diabetes and selected related conditions of six reservations in Montana and Wyoming.

    PubMed

    Acton, K; Rogers, B; Campbell, G; Johnson, C; Gohdes, D

    1993-01-01

    To describe the prevalence of diabetes and related complications in Plains Indians. The medical records of individuals with diagnosed diabetes seen in IHS facilities before 1 January 1987 were reviewed. Records were obtained from primary care clinics and hospitals accessible to the following six reservation communities in Montana and Wyoming: Blackfeet, Crow, Fort Belknap, Fort Peck, Northern Cheyenne, and Wind River. Medical records were reviewed to ascertain accuracy of diagnosis and associated complications. Age-adjusted prevalence rate of diagnosed diabetes was 119/1000 adults > or = 15 yr of age. Diabetes rates in Plains Indians are higher than rates described for the general U.S. population.

  1. Kootenai River Wildlife Habitat Enhancement Project : Long-term Bighorn Sheep/Mule Deer Winter and Spring Habitat Improvement Project : Wildlife Mitigation Project, Libby Dam, Montana : Management Plan.

    SciTech Connect

    Yde, Chis

    1990-06-01

    The Libby hydroelectric project, located on the Kootenai River in northwestern Montana, resulted in several impacts to the wildlife communities which occupied the habitats inundated by Lake Koocanusa. Montana Department of Fish, Wildlife and Parks, in cooperation with the other management agencies, developed an impact assessment and a wildlife and wildlife habitat mitigation plan for the Libby hydroelectric facility. In response to the mitigation plan, Bonneville Power Administration funded a cooperative project between the Kootenai National Forest and Montana Department of Fish, Wildlife and Parks to develop a long-term habitat enhancement plan for the bighorn sheep and mule deer winter and spring ranges adjacent to Lake Koocanusa. The project goal is to rehabilitate 3372 acres of bighorn sheep and 16,321 acres of mule deer winter and spring ranges on Kootenai National Forest lands adjacent to Lake Koocanusa and to monitor and evaluate the effects of implementing this habitat enhancement work. 2 refs.

  2. Lodgepole reef potential seen in Montana Williston basin

    SciTech Connect

    Brogdon, L.; Ball, S.M.; Ball, D.S.

    1996-12-16

    The Williston basin Mississippian Lodgepole oil play has suffered a string of dry holes lately eroding the confidence of explorationists to find these prolific reefs, particularly in North Dakota. Detailed mapping of the Lodgepole trend suggests more Lodgepole reefs will be found in the Montana part of the trend than in North Dakota. Companies seeking impact plays should certainly give this area strong consideration. The paper discusses the delineation of a lower Lodgepole fairway extending into Montana with identification of reef facies in key wells (reef clusters), good source rocks, high quality seismic data, and impact reserve potential which makes Montana good hunting ground for significant new discoveries.

  3. In vivo antioxidative activity of a quantified Pueraria lobata root extract.

    PubMed

    Bebrevska, Lidiya; Foubert, Kenne; Hermans, Nina; Chatterjee, Shyama; Van Marck, Eric; De Meyer, Guido; Vlietinck, Arnold; Pieters, Luc; Apers, Sandra

    2010-01-08

    Oxidative stress has been associated with many pathological disorders such as atherosclerosis, diabetes and cancer. Supplementation with exogenous antioxidants, including phenolic compounds from plant sources, may help to restore the pro-oxidative/antioxidative balance. To take into account effects of absorption, metabolisation, plasma protein binding, distribution, and elimination, antioxidative research should not be limited to in vitro assays but be extended to in vivo models. In the present work a quantified 50% EtOH root extract of Pueraria lobata (Willd.) Ohwi (Fabaceae) was selected to determine its in vivo antioxidative activity in a diabetic rat model, where diabetes and the accompanying oxidative stress were induced by intraperitoneal administration of streptozotocin. This root extract was found to contain 10.42+/-0.15% puerarin as the main constituent and smaller amounts of the related isoflavonoids 3'-hydroxypuerarin, 3'-methoxypuerarin, 6''-xylosylpuerarin, daidzin, genistin, daidzein and genistein, as determined by a validated HPLC method. This extract was administered orally at a daily dose of 500 mg/kg root extract, corresponding to 50mg/kg puerarin, during 3 weeks. In addition the effect on the plasma concentration of some fat-soluble antioxidants (co-enzyme Q(9), alpha- and gamma-tocopherol) was evaluated. The level of malondialdehyde (MDA) in plasma, used as a marker of oxidative damage to lipids, was reduced to the same level as in healthy control animals, and as in the positive control group treated daily with 50mg/kg alpha-tocopherol acetate. No obvious signs of toxicity were observed by administration of 10x the treatment dose. Copyright 2009 Elsevier Ireland Ltd. All rights reserved.

  4. Preventive effects of Pueraria mirifica on bone loss in ovariectomized rats.

    PubMed

    Urasopon, Nontakorn; Hamada, Yuzuru; Cherdshewasart, Wichai; Malaivijitnond, Suchinda

    2008-02-20

    Effects of Pueraria mirifica on bone loss in fully mature ovariectomized rats are examined. Two series of experiments were performed. In the first series, rats were kept with their ovaries intact and divided into two groups; initial control (IC) and sham control (SH). The IC rats were sacrificed on day 1 and their data were kept as baseline control. The SH rats were subjected to sham operation on day 0 and gavaged daily with distilled water for 90 days. In the second series, rats were subjected to ovariectomy, divided into five groups and gavaged daily with 0.1mg/kg B.W./day of 17-alpha-ethinylestradiol (EE), 0, 10, 100 and 1000mg/kg B.W./day of P. mirifica (P0, P10, P100 and P1000, respectively) for 90 days. Changes of bone mineral density and bone mineral content were measured using peripheral Quantitative Computerized Tomography. Bone loss was significantly induced by ovariectomy and it was dose-dependently prevented by P. mirifica treatment for 90 days. The preventive effects of P. mirifica on bone loss depended on bone types (axial or long bone), bone sites (metaphysis or diaphysis), and bone compartments (trabecular and cortical). At P100 and P1000, bone loss was completely prevented both in trabecular bone mineral density and content. The effects of P. mirifica were, as expected, comparable to that in the EE group. These results suggest that P. mirifica may be applicable to treat the osteoporosis in menopausal women; however, an undesirable side effect on stimulating reproductive organs should be concerned.

  5. Molecular Docking and Interactions of Pueraria Tuberosa with Vascular Endothelial Growth Factor Receptors

    PubMed Central

    Asthana, S.; Agarwal, T.; Singothu, S.; Samal, A.; Banerjee, I.; Pal, K.; Pramanik, K.; Ray, S. S.

    2015-01-01

    Pueraria tuberosa is known for its therapeutic potentials in cardiovascular disorders, but its effect in angiogenesis has not been studied so far. In this study, a computational approach has been applied to elucidate the role of the phytochemicals in inhibition of angiogenesis through modulation of vascular endothelial growth factor receptors: Vascular endothelial growth factor receptor-1 and vascular endothelial growth factor receptor-2, major factors responsible for angiogenesis. Metabolite structures retrieved from PubChem and KNApSAcK – 3D databases, were docked using AutoDock4.2 tool. Hydrogen bond and molecular docking, absorption, distribution, metabolism and excretion and toxicity predictions were carried out using UCSF Chimera, LigPlot+ and PreADMET server, respectively. From the docking analysis, it was observed that puerarone and tuberostan had significant binding affinity for the intracellular kinase domain of vascular endothelial growth factor receptors-1 and vascular endothelial growth factor receptor-2 respectively. It is important to mention that both the phytochemicals shared similar interaction profile as that of standard inhibitors of vascular endothelial growth factor receptors. Also, both puerarone and tuberostan interacted with Lys861/Lys868 (adenosine 5’-triphosphate binding site of vascular endothelial growth factor receptors-1/vascular endothelial growth factor receptors-2), thus providing a clue that they may enforce their inhibitory effect by blocking the adenosine 5’-triphosphate binding domain of vascular endothelial growth factor receptors. Moreover, these molecules exhibited good drug-likeness, absorption, distribution, metabolism and excretion properties without any carcinogenic and toxic effects. The interaction pattern of the puerarone and tuberostan may provide a hint for a novel drug design for vascular endothelial growth factor tyrosine kinase receptors with better specificity to treat angiogenic disorders. PMID:26664060

  6. Potentiation of the bioavailability of daidzin by an extract of Radix puerariae.

    PubMed

    Keung, W M; Lazo, O; Kunze, L; Vallee, B L

    1996-04-30

    The dose effect of pure daidzin on the suppression of ethanol intake in Syrian golden hamsters was compared with that of crude daidzin contained in a methanol extract of Radix puerariae (RP). EC50 values estimated from the graded dose-response curves for pure daidzin and RP extract daidzin are 23 and 2.3 mg per hamster per day, respectively. Apparently the antidipsotropic activity of the RP extract cannot be accounted for solely by its daidzin content (22 mg/g). In addition to daidzin, six other isoflavones were identified in the RP extract and quantified--namely, puerarin (160 mg per g of extract), genistin (3.7 mg/g), daidzein (2.6 mg/g), daidzein-4',7-diglucoside (1.2 mg/g), genistein (0.2 mg/g), and formononetin (0.16 mg/g). None of these, administered either alone or combined, contributes in any significant way to the antidipsotropic activity of the extract. Plasma daidzin concentration-time curves determined in hamsters administered various doses of pure daidzin or RP extract by i.p.injection indicate that the crude extract daidzin has approximately 10 times greater bioavailability than the pure compound. Reconstruction of the dose-response effects for pure and crude daidzin using bioavailable daidzin rather than administered dose gives a single curve. Synthetic daidzin added to the RP extract acquires the bioavailability of the endogenous daidzin that exists naturally in the extract. These results show that (i) daidzin is the major active principle in methanol extracts of RP, and (ii) additional constituents in the methanol extract of RP assist uptake of daidzin in golden hamsters.

  7. Melanogenesis inhibitory effect of aerial part of Pueraria thunbergiana in vitro and in vivo.

    PubMed

    Han, EunByeol; Chang, BoYoon; Kim, DaeSung; Cho, HyoungKwon; Kim, SungYeon

    2015-01-01

    Melanin is major factor that determines skin color as well as one of the defense systems that prevent the UV-induced damage. In case of abnormal concentration of melanin, skin diseases or problems occur such as albinism, leukoplakia, melasma, freckles, moles, and lentigo. With the lifespan of humans has been extended, importance of 'life quality' has been increased. White and clean skin is very important part of the satisfaction of appearance, especially for Asia women. The aim of this study was to find an anti-melanogenesis activity for which the aerial part of Pueraria thunbergiana can be utilized based on the increase in demands for cosmetics, particularly natural products. We demonstrated anti-pigmentation effects of aerial part of P. thunbergiana by measuring melanin content and through staining in the B16F10 melanoma cell line. The aerial part of P. thunbergiana decreased tyrosinase activity significantly in B16F10 cell cultures, while there is no direct effect on enzyme in cell-free conditions. To define the mechanisms, real-time PCR, western blot, glucosidase activity and antioxidant activity assay were implemented. As results, we demonstrated that aerial part of P. thunbergiana has anti-melanogenesis activity via two mechanisms. One is downgrading microphthalmia-associated transcription factor by activating Akt/GSK-3β. Consequently, transcription of tyrosinase and tyrosinase-related protein 1 is decreased. Another is interrupting maturation of tyrosinase through inhibiting α-glucosidase. Furthermore, aerial part of P. thunbergiana showed great efficacy on pigmentation in vivo. These results suggest that aerial part of P. thunbergiana can be used as an anti-melanogenic agent.

  8. Preparation of monomeric and polymeric β-cyclodextrin functionalized monoliths for rapid isolation and purification of puerarin from Radix puerariae.

    PubMed

    Lv, Yongqin; Hughes, Timothy C; Hao, Xiaojuan; Mei, Danping; Tan, Tianwei

    2011-08-01

    Monomeric and epichlorohydrin polymerized β-CD functionalized monoliths were prepared for the rapid isolation and purification of the isoflavonoid puerarin, a well-known traditional Chinese drug, from a crude extract of Radix puerariae (root of the plant Pueraria lobata). Two copolymers poly(isocyanatoethyl methacrylate-co-methyl methacrylate-co-ethylene dimethacrylate) (poly(IEM-co-MMA-co-EDMA)) and poly(glycidyl methacrylate-co-EDMA) (poly(GMA-co-EDMA)) were developed as facile, highly reactive and versatile monolithic matrix. SEM characterization demonstrated that the modified monoliths had homogenous porous structure and morphology. The success of the chemical modification of the monolithic matrix was confirmed by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), solid-state (13) C NMR and elemental analysis. It was demonstrated that polymeric β-CD modified monoliths had better separation and selectivity for puerarin, recovering puerarin with a purity of 96% (m%) and a yield of 93% (m%). Compared with poly(glycidyl methacrylate-co-EDMA), poly(isocyanatoethyl methacrylate-co-methyl methacrylate-co-EDMA) monolithic matrix had higher reactivity, which significantly improved the β-CD ligand density and thus the selectivity of the monoliths. Puerarin with a purity of 96% (m%) and with a yield of 89% (m%) was recovered on the monolith.

  9. Breccia dikes from the Beaverhead Impact structure, southwest Montana

    NASA Technical Reports Server (NTRS)

    Fiske, P. S.; Hougen, S. B.; Hargraves, R. B.

    1992-01-01

    While shatter cones are generally accepted as indicators of meteorite impact, older petrologic features are not widely recognized in the geologic community. Breccia dikes are one such feature. They are found in many large impact structures occurring over an area at least as extensively as shatter cones. Breccia dikes will survive moderate degrees of metamorphism and tectonism, unlike many other microscopic features (shocked quartz grains, high-pressure polymorphs, etc.) and even large-scale features such as annular or bowl-shaped topographic features. Thus, they are important diagnostic criteria, especially for large, poorly preserved impact structures. The Beaverhead Impact structure is a recently discovered, deeply eroded impact structure in southwestern Montana. The remains of the structure are delineated by the occurrence of shatter cones, found in an area greater than 200 sq km, occurring within the Cabin thrust plate, part of the Cretaceous Sevier fold and thrust system. The distribution of shatter cones is further truncated by Tertiary normal faults. The present remains represent an allochthonous fragment of a larger structure.

  10. The differential anti-proliferation effect of white (Pueraria mirifica), red (Butea superba), and black (Mucuna collettii) Kwao Krua plants on the growth of MCF-7 cells.

    PubMed

    Cherdshewasart, W; Cheewasopit, W; Picha, P

    2004-08-01

    The differential anti-proliferation effect of white (Pueraria mirifica), red (Butea superba) and black (Mucuna collettii) Kwao Krua plant extracts on the growth of MCF-7 cells was evaluated after 4 days of incubation. The percent cell growth comparison was based on protein determination of the harvested cells in parallel with the control group and Pueraria lobata treatment group. Pueraria lobata led to no proliferation and a mild anti-proliferation effect on the growth of MCF-7 cells. Pueraria mirifica caused proliferation at 1 microg/mL and an anti-proliferative effect on the growth of MCF-7 cells at 100 and 1000 microg/mL with an ED50 value of 642.83 microg/mL. Butea superba led to no proliferation and an anti-proliferation effect on the growth of MCF-7 cells at 10, 100 and 1000 microg/mL with an ED50 value of 370.91 microg/mL. Mucuna collettii led to no proliferation and an anti-proliferation effect on the growth of MCF-7 cells at 100 and 1000 microg/mL with an ED50 value of 85.36 microg/mL. The results demonstrated that only Pueraria mirifica showed an estrogenic effect on MCF-7 cell growth and a clear antagonistic effect with E2 at high concentration. Butea superba and Mucuna collettii exhibited only anti-proliferation effects on the growth of MCF-7 cells in relation with a possible anti-estrogen mechanism or a potent cytotoxic effect.

  11. Fire ecology of western Montana forest habitat types

    Treesearch

    William C. Fischer; Anne F. Bradley

    1987-01-01

    Provides information on fire as an ecological factor for forest habitat types in western Montana. Identifies Fire Groups of habitat types based on fire's role in forest succession. Describes forest fuels and suggests considerations for fire management.

  12. Northwest Montana Wildlife Mitigation Habitat Protection : Advance Design : Final Report.

    SciTech Connect

    Wood, Marilyn A.

    1993-02-01

    This report summarizes the habitat protection process developed to mitigate for certain wildlife and wildlife habitat losses due to construction of Hungry Horse and Libby dams in northwestern Montana.

  13. 1. View looking south on Montana Street. The Silver Bow ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View looking south on Montana Street. The Silver Bow County Courthouse (1910-1912) is on the left. - Butte Historic District, Bounded by Copper, Arizona, Mercury & Continental Streets, Butte, Silver Bow County, MT

  14. Bone foreshafts from a clovis burial in southwestern montana.

    PubMed

    Lahren, L; Bonnichsen, R

    1974-10-11

    Formal and functional analyses of bone artifacts from a Clovis burial in southwestern Montana suggest that they were constructed to serve as (detachable or nondetachable) foreshafts for attaching fluted projectile points to lance shafts.

  15. Montana's 1988 fuelwood harvest. Forest Service Resource Bulletin

    SciTech Connect

    McLain, W.H.

    1990-10-01

    The report highlights the 1988 harvest of fuelwood in Montana by Commercial fuelwood harvesters and those cutting for home consumption. It lists a directory of commercial fuelwood harvesters and describes the methods of data collection and compilation.

  16. 76 FR 38604 - Southern Montana Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    ... in Big Timber, Montana. The committee is meeting as authorized under the Secure Rural Schools and... Public Library, 34 McLeod Street, Big Timber, MT. Written comments should be sent to Babete Anderson...

  17. 76 FR 44032 - Montana; Major Disaster and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-22

    ... beginning on April 4, 2011, and continuing, is of sufficient severity and magnitude to warrant a major... Montana have been designated as adversely affected by this major disaster: Big Horn, Blaine,...

  18. Ice Jams in Montana. Ice Engineering. Number 19, July 1998

    DTIC Science & Technology

    1998-07-01

    numerous livestock drowned, many homes were washed away, and several spans of the Yellowstone River Bridge were destroyed during this event. These...Fig. 2). The earliest entry in the Ice Jam Database for Montana is an 1894 event on the Yellowstone River at Glendive. The most recent entries are from...locations on 119 different rivers in Montana (Fig. 2). The localities with the most recorded ice jam events (32) are Miles City on the Yellowstone River

  19. Sources and patterns of wolverine mortality in western Montana

    Treesearch

    John R. Squires; Jeffrey P. Copeland; Todd J. Ulizio; Michael K. Schwartz; Leonard F. Ruggiero

    2007-01-01

    We instrumented 36 wolverines (Gulo gulo) on 2 study areas in western Montana and one study area on the Idaho-Montana (USA) border: 14 (9 M, 5 F) on the Pioneer study area, 19 (11 M, 8 F) on the Glacier study area, and 3 (2M, 1 F) on the Clearwater study area. During 2002-2005, harvest from licensed trapping accounted for 9 (6 M, 3 F) of 14 mortalities,...

  20. TEN LAKES WILDERNESS STUDY AREA, MONTANA.

    USGS Publications Warehouse

    Whipple, James W.; Hamilton, Michael M.

    1984-01-01

    A mineral-resource survey of the Ten Lakes Wilderness Study Area, Montana, was conducted. Areas of probable or substantiated mineral potential were found surrounding zones of past mining activity east of Independence Peak where copper-bearing veins are hosted by basaltic lava flows. Three mines contain demonstrated or inferred resources, and there are numerous prospects. Other areas of probable resource potential include an area on Sinclair Creek with copper occurrences similar to those of the Independence Peak area, an area including Gilbralter Ridge where lead and zinc veins are hosted by sedimentary rocks, two areas where zinc-copper occurrences are related to metadiorite sills, and two areas that contain stratabound copper occurrences. The areas with copper and lead-zinc veins may also be of interest for deeply buried mineralized systems, as the veins may be the surface expression of plutons at relatively shallow depths.

  1. US hydropower resource assessment for Montana

    SciTech Connect

    Francfort, J.E.

    1993-12-01

    The Department of Energy is developing an estimate of the hydropower development potential in this country. The Hydropower Evaluation Software (HES) is a computer model that was developed by the Idaho National Engineering Laboratory for this purpose. The HES measures the potential hydropower resources available in the United States, using uniform criteria for measurement. The software was developed and tested using hydropower information and data provided by the Southwestern Power Administration. It is a dBASE menu-driven software application that allows the personal computer user to assign environmental attributes to potential hydropower sites, calculate development suitability factors for each site based on the environmental attributes present, and generate reports based on these suitability factors. This report details the resource assessment results for the state of Montana.

  2. Applicability of ERTS-1 to Montana geology

    NASA Technical Reports Server (NTRS)

    Weidman, R. M. (Principal Investigator); Alt, D. D.; Berg, R.; Johns, W.; Flood, R.; Hawley, K.; Wackwitz, L.

    1976-01-01

    The author has identified the following significant results. Late autumn imagery provides the advantages of topographic shadow enhancement and low cloud cover. Mapping of rock units was done locally with good results for alluvium, basin fill, volcanics, inclined Paleozoic and Mesozoic beds, and host strata of bentonite beds. Folds, intrusive domes, and even dip directions were mapped where differential erosion was significant. However, mapping was not possible for belt strata, was difficult for granite, and was hindered by conifers compared to grass cover. Expansion of local mapping required geologic control and encountered significant areas unmappable from ERTS imagery. Annotation of lineaments provided much new geologic data. By extrapolating test site comparisons, it is inferred that 27 percent of some 1200 lineaments mapped from western Montana represent unknown faults. The remainder appear to be localized mainly by undiscovered faults and sets of minor faults or joints.

  3. National uranium resource evaluation, Kalispell Quadrangle, Montana

    SciTech Connect

    Fleshman, B.R.; Siegmund, B.L.

    1982-03-01

    The Kalispell Quadrangle, Montana, was evaluated using criteria developed for the National Uranium Resource Evaluation program for the purpose of identifying and delineating areas containing geologic environments favorable for uranium deposits. Reconnaissance and detailed investigations included the evaluation of reported uranium occurrences, geochemical sampling, and field evaluation of hydrogeochemical and stream-sediment anomalies. Results of the investigations indicate that two areas have potential for uranium deposits in or related to plutonic rocks. Both areas contain peralkaline plutonic rocks and are possibly spatially and genetically related to a larger alkaline-ultramafic complex. The nonradioactive plutonic rocks of the Bobtail stock are possibly related to this larger complex but are considered unfavorable for uranium deposits. Precambrian volcanic and metasedimentary rocks outside areas containing polymetallic vein-type silver-lead-zinc enrichment are also considered unfavorable for uranium deposits. Paleozoic, Mesozoic, and Cenozoic sedimentary rocks and Quaternary deposits are also unfavorable.

  4. FLINT CREEK RANGE WILDERNESS STUDY AREA, MONTANA.

    USGS Publications Warehouse

    Ericksen, George E.; Marks, Lawrence Y.

    1984-01-01

    A mineral survey of the Flint Creek Range Wilderness study area, Montana shows the presence of mineral deposits. By far the most important are low-grade, potentially large, contact-metamorphic tungsten deposits. A large stockwork molybdenum deposit is probably low in grade. The areas of these tungsten and molybdenum deposits have substantiated mineral-resource potential. A multimillion ton phosphate-rock deposit occurs in an area of substantiated resource potential in the Permian Phosphoria Formation in the south-central part of the study area. Deposits of massive quartz, perhaps suitable for smelter flux, a demonstrated resource. Small scattered silver- and gold-bearing veins are present, but no resource potential was identified.

  5. Applicability of ERTS-1 to Montana geology

    NASA Technical Reports Server (NTRS)

    Weidman, R. M. (Principal Investigator)

    1973-01-01

    The author has identified the following significant results. Geologic maps of four test sites were compiled at 1/250,000. Band 7 prints enlarged to 1/500,000 scale are the best for the purpose, and negative prints provide a valuable supplement. More than 100 mapped lineaments represent most of the major faults of the area and a large number of suspected faults, including many of northeast trend. Under ideal conditions dip slopes may be recognized, laccoliths outlined, and axial traces drawn for narrow, plunging folds. Use of ERTS-1 imagery will greatly facilitate construction of a needed tectonic map of Montana. From ERTS-1 imagery alone, it was possible to identify up-turned undivided Paleozoic and Mesozoic strata and to map the boundaries of mountain glaciation, intermontane basins, a volcanic field, and an area of granitic rocks. It was also possible to outline clay pans associated with bentonite. However, widespread recognition of gross rock types will be difficult.

  6. [Apoptosis of Kasumi-1 cells induced by puerariae radix flavones and its molecular mechanism].

    PubMed

    Shao, Hua-min; Tang, Yu-hong; Shen, Qun; Zhu, Hong-qing; Ji, Ou; Zhang, Ya-cheng; Ji, Jian-min; Jiang, Peng-jun; Si, Ye-jun; Li, Zhao-rong

    2012-01-01

    To explore the effects and the molecular mechanism of puerariae radix flavones (PRF) on acute myeloid leukemia cell line Kasumi-1 cells in vitro. Kasumi-1 cells treated by PRF for 48 hours were observed with Wright's and Hoechst 33258 dying. The apoptotic cells were analyzed by flow cytometry with AnnexinV/PI staining. The expression levels of bcl-2, Bim and Caspase-3/-8/-9 protein were assayed by Western blot and the AML1-ETO fusion gene was detected by real-time polymerase chain reaction. PRF could induce Kasumi-1 cells to apoptosis effectively. The proportion of apoptotic cells in 50, 200 and 500 µg/ml PRF treatment groups were (14.1 ± 0.8)%, (17.7 ± 1.3)% and (32.4 ± 1.4)%, respectively, and significantly higher than that of control \\[(7.8 ± 0.7)%\\]. The relative expression levels of the anti-apoptotic Bcl-2 protein were 0.85 ± 0.05, 0.62 ± 0.07 and 0.43 ± 0.05; the apoptotic Bim protein were 0.21 ± 0.06, 0.39 ± 0.04 and 0.75 ± 0.05; the caspase-3 and caspase-9 were 0.92 ± 0.04, 1.21 ± 0.07, 1.33 ± 0.04 and 0.35 ± 0.05, 0.53 ± 0.03, 0.69 ± 0.07, respectively. Compared to the blank control group, all these changes were significant (P < 0.01). Nevertheless, nearly no changes could be observed on the expression level of AML1-ETO fusion gene and caspase-8 protein. Apoptosis of Kasumi-1 cells induced by PRF might correlate to the down-regulation of Bcl-2 protein expression and the activation of caspase-3 and caspase-8 protein in the cells. It seemed that all these effects had no relationship with the AML1-ETO fusion gene.

  7. Effect of Pueraria tuberosa tuber extract on chronic foot shock stress in Wistar rats.

    PubMed

    Pramanik, S S; Sur, T K; Debnath, P K; Bhattacharyya, D

    2010-12-01

    The present study was undertaken to explore the protective effects of tuberous root extract of Pueraria tuberosa on chronic foot shock stress (CS) induced physiological, neurobehavioral and neuropathological alterations. Male Wistar rats (120-150 g) were divided into seven groups, consisting of ten animals in each. Group I served as normal, group II as positive control, while group III-VII as test drug treated. P tuberosa tuber extract (PTE) was given to rats of groups III-VI at the doses of 50, 100, 200 and 400 mg/kg respectively, while group VII treated with Withania somnifera rhizome extract (WSE) (100 mg/kg) as reference drug. Group II (stress control) received only equivalent volume of distilled water (0.5 ml/100 g) orally. All the drugs were given orally once/day for 14 consecutive days. The last dose was given 1 h before study. Simultaneously, all the animals (except group I) were subjected to 1 h of foot-shock (2 mA) through a grid floor for those 14 days in a standard conditioning chamber with the escape route closed [Chronic stress (CS)]. Thereafter, the rats were placed on open-field and plus maze apparatus for studying the behavioral patterns of them, and the anxiolytic effects of the putative drug. Sexual activities of the animals were also studied. Finally, the animals were sacrificed and their ulcer formation in gastric mucosa was noted. Weights of adrenals and spleen were also taken. Further, plasma corticosterone levels were estimated spectroflurometrically. Results indicated that, CS significantly altered the behavioral patterns, decreased the sexual urge and activities, damaged the gastric mucosal layers, enhanced plasma corticosterone levels and increased adrenal glands and spleen weights. PTE and WSE showed significant anxiolytic activity, protected the gastric mucosa, lowered plasma corticosterone level (indicating HPA axis inhibition) and negated the hypertrophy of adrenals and spleen. PTE also enhanced the sexual urge and activities in

  8. Flos Puerariae extract prevents myocardial apoptosis via attenuation oxidative stress in streptozotocin-induced diabetic mice.

    PubMed

    Yu, Wei; Zha, Wenliang; Guo, Shuang; Cheng, Hongke; Wu, Jiliang; Liu, Chao

    2014-01-01

    Diabetic cardiomyopathy (DCM) suggests a direct cellular insult to myocardium. Apoptosis is considered as one of the hallmarks of DCM. Oxidative stress plays a key role in the pathogenesis of DCM. In this study, we explored the prevention of myocardial apoptosis by crude extract from Flos Puerariae (FPE) in experimental diabetic mice. Experimental diabetic model was induced by intraperitoneally injection of streptozotocin (STZ, 50 mg/kg/day) for five consecutive days in C57BL/6J mice. FPE (100, 200 mg/kg) was orally administrated once a day for ten weeks. Cardiac structure changes, apoptosis, superoxide production, NADPH oxidase subunits expression (gp91phox, p47phox, and p67phox), and related regulatory factors were assessed in the heart of mice. Diabetic mice were characterized by high blood glucose (≥11.1 mmol/L) and reduced body weight. In the end of the experiment, aberrant myofilament structure, as well as TUNEL positive cardiac cells coupled with increased Bax/Bcl-2 ratio and Caspase-3 expression was found in diabetic mice. Moreover, ROS formation, the ratio of NADP+/NADPH and NADPH oxidase subunits expression of gp91phox and p47phox, lipid peroxidation level was significantly increased, while antioxidant enzyme SOD and GSH-Px activity were reduced in the myocardial tissue of diabetic mice. In contrast, treatment with FPE resulted in a normalized glucose and weight profile. FPE administration also preserved myocardial structure and reduced apoptotic cardiac cell death in diabetic mice. The elevated markers of oxidative stress were significantly reversed by FPE supplementation. Further, FPE treatment markedly inhibited the increased Bax/Bcl-2 ratio and Caspase-3 expression, as well as suppressed JNK and P38 MAPK activation in the heart of diabetic mice. Our data demonstrate for the first time that FPE may have therapeutic potential for STZ-induced diabetic cardiomyopathy through preventing myocardial apoptosis via attenuation oxidative stress. And this

  9. Molecular clusters size of Puerariae thomsonii radix aqueous decoction and relevance to oral absorption.

    PubMed

    Wang, Gong; Yang, Caimei; Zhang, Kuan; Hu, Juan; Pang, Wensheng

    2015-07-07

    The multi-component system of traditional Chinese medicine (TCM) is very complicated. The clusters are dynamic aggregates whose molecules are held together by hydrogen-bonded, Van der Waals forces or the opposite charges of particles attract each other. In this paper, field emission scanning electron microscopy proved that molecules form clusters in Pueraria thomsonii Benth (Fenge) water decoction. Four kinds of Fenge water decoction, 0.07 g∙mL-1 (F-1), 0.1 g∙mL-1 (F-2), 0.17 g∙mL-1 (F-3), 0.35 g∙mL-1 (F-4); F-1, average diameter of molecular was about 120 nm; F-2, 195 nm; F-3, 256 nm; and F-4, 480 nm. The molecular size was shown to depend on concentration. Rabbits were given equal does of 2.8 g∙kg-1, to perfuse F-1, F-2, F-3, F-4 in volume of 80 mL, 56 mL, 33 mL, 17 mL, respectively. At 0-180 min to collect 2 mL blood from the rabbit ears middle arteries for metabolism fingerprints, the results show the particle size of molecular is smaller, the absorption of drugs is better instead. The acute blood stasis model rats were treatment with Fenge decoction of 1.5 g∙kg-1 for 14 days, the concentrations of Ang II in plasma were significantly lower in F-1 and F-2 groups than those in model group (p < 0.01 or p < 0.05), but there were no significantly difference in F-3 and F-4 groups than those in model group (p > 0.05). Despite the molecular aggregation is a common physical phenomenon, it influence on the kind and amount of molecule per unit volume. Molecules morphology influence on the absorption behavior of drugs in vivo therefore is to have an impact on pharmacological function.

  10. Montana StreamStats—A method for retrieving basin and streamflow characteristics in Montana: Chapter A in Montana StreamStats

    USGS Publications Warehouse

    McCarthy, Peter M.; Dutton, DeAnn M.; Sando, Steven K.; Sando, Roy

    2016-04-05

    The U.S. Geological Survey (USGS) provides streamflow characteristics and other related information needed by water-resource managers to protect people and property from floods, plan and manage water-resource activities, and protect water quality. Streamflow characteristics provided by the USGS, such as peak-flow and low-flow frequencies for streamflow-gaging stations, are frequently used by engineers, flood forecasters, land managers, biologists, and others to guide their everyday decisions. In addition to providing streamflow characteristics at streamflow-gaging stations, the USGS also develops regional regression equations and drainage area-adjustment methods for estimating streamflow characteristics at locations on ungaged streams. Regional regression equations can be complex and often require users to determine several basin characteristics, which are physical and climatic characteristics of the stream and its drainage basin. Obtaining these basin characteristics for streamflow-gaging stations and ungaged sites traditionally has been time consuming and subjective, and led to inconsistent results.StreamStats is a Web-based geographic information system application that was created by the USGS to provide users with access to an assortment of analytical tools that are useful for water-resource planning and management. StreamStats allows users to easily obtain streamflow and basin characteristics for USGS streamflow-gaging stations and user-selected locations on ungaged streams. The USGS, in cooperation with Montana Department of Transportation, Montana Department of Environmental Quality, and Montana Department of Natural Resources and Conservation, completed a study to develop a StreamStats application for Montana, compute streamflow characteristics at streamflow-gaging stations, and develop regional regression equations to estimate streamflow characteristics at ungaged sites. Chapter A of this Scientific Investigations Report describes the Montana Stream

  11. 76 FR 31579 - Designation for the State of Georgia and State of Montana Areas

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-01

    ...) and the Montana Department of Agriculture (Montana) to provide official services under the United... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Grain Inspection, Packers and Stockyards Administration Designation for the State of Georgia and...

  12. 77 FR 35061 - Roseburg Forest Products Composite Panels Division Missoula, Montana; Notice of Negative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Roseburg Forest Products Composite Panels Division Missoula, Montana... former workers of Roseburg Forest Products, Composite Panels Division, Missoula, Montana (subject firm...

  13. 75 FR 57059 - Montana Department of Natural Resources and Conservation Final Habitat Conservation Plan and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-17

    ... Fish and Wildlife Service Montana Department of Natural Resources and Conservation Final Habitat Conservation Plan and Final Environmental Impact Statement AGENCY: Fish and Wildlife Service, Interior. ACTION... received from the Montana Department of Natural Resources and Conservation (DNRC) a Final...

  14. Communities.

    ERIC Educational Resources Information Center

    Ruth, Amy, Ed.

    1995-01-01

    This issue of the Goldfinch focuses on communities. Ethnic groups, religious groups, schools, families, even the Internet, are used as examples of communities in Iowa. Forced communities are exemplified by migration, law, natural disasters, and sometimes education. Photographs from Iowa's past show the changing nature of communities and encourage…

  15. Increased puerarin biosynthesis during in vitro shoot formation in Pueraria tuberosa grown in growtek bioreactor with aeration.

    PubMed

    Sharma, Varsha; Goyal, Shaily; Ramawat, Kishan G

    2011-03-01

    Puerarin accumulation during shoot cultures in static and liquid medium with or without aeration is described in Pueraria tuberosa (Roxb. ex Willd.) DC. Maximum shoot induction from nodal explants was achieved on Murashige and Skoog's medium supplemented with 1.13 μM TDZ and 0.25 μM IBA after 4 weeks of growth. Puerarin content was higher in shoot cultures grown in liquid medium as compared to static medium. When shoots were grown in growtek bioreactor with different aeration volume, maximum puerarin content (1484 μg/g DW) was recorded with 20 % v/v aeration which was ~2.3 fold higher than puerarin content recorded in control cultures (cultures grown in growtek without aeration). Aeration requirement for organized (shoot) cultures was different for growth and puerarin accumulation.

  16. Inhibition of aldose reductase and xylose-induced lens opacity by puerariafuran from the roots of Pueraria lobata.

    PubMed

    Kim, Nan Hee; Kim, Young Sook; Lee, Yun Mi; Jang, Dae Sik; Kim, Jin Sook

    2010-01-01

    High sugar levels found in diabetic cataract cause the opacification of lenses by osmotic changes induced via the aldose reductase (AR)-mediated polyol pathway. In this study, puerariafuran, a 2-arylbenzofuran from Pueraria lobata, investigated the inhibitory effects upon AR, antioxidant contents and enzyme activities in the lens. The effect of puerariafuran on xylose-induced lens opacity was also examined. Puerariafuran showed potential inhibitory activity with an IC50 value of 22.34 microM against rat lens AR. The xylose-induced opacity of lenses was significantly improved when treated with puerariafuran. Xylose exposure of rat lenses significantly decreased the reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, superoxide dismutase (SOD), and catalase (CAT) activity and treatment with puerariafuran significantly increased these factors. These results suggest that puerariafuran may provide a potential therapeutic approach for prevention of diabetic complications, such as cataracts.

  17. Suppression of BSEP and MRP2 in mouse liver by miroestrol and deoxymiroestrol isolated from Pueraria candollei.

    PubMed

    Udomsuk, Latiporn; Juengwatanatrakul, Thaweesak; Putalun, Waraporn; Jarukamjorn, Kanokwan

    2012-11-15

    Miroestrol and deoxymiroestrol are highly active phytoestrogens isolated from the tuberous root of Pueraria candollei var. mirifica (Leguminosae). Modulatory effects of miroestrol and deoxymiroestrol on the mRNAs of BSEP and MRP2 genes involved in bile salt transportation, in C57BL/6 mice were investigated. In contrast to estradiol, miroestrol and deoxymiroestrol suppressed the expression of BSEP and MRP2 mRNA in both male and female mice. The results suggest for the first time that the use of miroestrol and deoxymiroestrol-containing products as alternative medicines or health supplements should be concerned according to their effects on key genes that regulate the bile salt export pump, which could result in the risk of hepatotoxicity and intrahepatic cholestasis.

  18. Rapid Determination of Puerarin by Near-infrared Spectroscopy During Percolation and Concentration Process of Puerariae Lobatae Radix

    PubMed Central

    Jintao, Xue; Quanwei, Yang; Yun, Jing; Yufei, Liu; Chunyan, Li; Jing, Yang; Yanfang, Wu; Peng, Li; Guangrui, Wan

    2016-01-01

    Background: Gegen (Puerariae Labatae Radix) is one of the important medicines in Traditional Chinese Medicine. The studies showed that Gegen and its preparation had effective actions for atherosclerosis. Objective: Near-infrared (NIR) was used to develop a method for rapid determination of puerarin during percolation and concentration process of Gegen. Materials and Methods: About ten batches of samples were collected with high-performance liquid chromatography analysis values as reference, calibration models are generated by partial least-squares (PLS) regression as linear regression, and artificial neural networks (ANN) as nonlinear regression. Results: The root mean square error of prediction for the PLS and ANN model was 0.0396 and 0.0365 and correlation coefficients (r2) was 97.79% and 98.47%, respectively. Conclusions: The NIR model for the rapid analysis of puerarin can be used for on-line quality control in the percolation and concentration process. SUMMARY Near-infrared was used to develop a method for on-line quality control in the percolation and concentration process of GegenCalibration models are generated by partial least-squares (PLS) regression as linear regression and artificial neural networks (ANN) as non-linear regressionThe root mean square error of prediction for the PLS and ANN model was 0.0396 and 0.0365 and correlation coefficients (r2) was 97.79% and 98.47%, respectively. Abbreviations used: NIR: Near-Infrared Spectroscopy; Gegen: Puerariae Loabatae Radix; TCM: Traditional Chinese Medicine; PLS: Partial least-squares; ANN: Artificial neural networks; RMSEP: Root mean square error of validation; R2: Correlation coefficients; PAT: Process analytical technology; FDA: The Food and Drug Administration; Rcal: Calibration set; RMSECV: Root mean square errors of cross-validation; RPD: Residual predictive deviation; SLS: Straight Line Subtraction; MLP: Multi-Layer Perceptron; MSE: Mean square error. PMID:27601848

  19. Oral toxicological studies of pueraria flower extract: acute toxicity study in mice and subchronic toxicity study in rats.

    PubMed

    Takano, Akira; Kamiya, Tomoyasu; Tsubata, Masahito; Ikeguchi, Motoya; Takagaki, Kinya; Kinjo, Junei

    2013-11-01

    Kudzu has been widely used as an herbal medicine in China. The root of the kudzu is also well known as an antipyretic and analgesic in treatment of the common cold, while its flower has been used to treat alcohol intoxication, alcohol abuse, and dysentery. Pueraria flower extract (PFE) is a hot water extract derived from the flower of the kudzu, Pueraria thomsonii Benth. (Fabaceae), oral intake of which exhibits anti-obesity properties in mice and humans. In this study, we conducted acute and subchronic toxicity studies for an evaluation of safety. In the acute study, PFE (5 g/kg body weight) was orally administered to ddY mice. For 14 d after administration, no deaths or abnormal changes were observed in general signs, body weight (BW), or food consumption, and no abnormal findings were observed in the major organs and tissues of either males or females at necropsy. The oral LD50 of PFE was therefore estimated to be higher than 5 g/kg BW. In the subchronic study, PFE was mixed into the diet in place of powdered CRF-1 and administered at concentrations of 0% (control), 0.5%, 1.5%, and 5.0% to male and female Sprague-Dawley rats for 90 d. No mortality or toxicological changes were observed during the experimental period. Blood biochemical, hematological, and urinary parameters revealed no toxicologically significant changes. Furthermore, no anatomical or histopathological changes due to PFE were observed. The no-observed adverse-effect-level of PFE was thus estimated to be 5.0% in the diet (male: 3.0 g/kg BW/d; female: 3.5 g/kg BW/d).

  20. Active saponins from root of Pueraria peduncularis (Grah. ex Benth.) Benth. and their molluscicidal effects on Pomacea canaliculata.

    PubMed

    Yang, ChunPing; Zhang, Min; Lei, Bo; Gong, GuoShu; Yue, GuiZhou; Chang, XiaoLi; Sun, XiaoFang; Tian, Yue; Chen, HuaBao

    2017-06-01

    Pueraria peduncularis (Grah. ex Benth.) Benth., which belongs to the Leguminosae family, exhibits resistance to many crop pests in agricultural production. Pomacea canaliculata is an important invasive snail in rice fields and causes severe yield losses. To evaluate the toxicity of P. peduncularis to P. canaliculata, in this study the molluscicidal activity of root extracts of P. peduncularis was tested against P. canaliculata; the active compounds were isolated, and the structures of these compounds were analysed using nuclear magnetic resonance (NMR) analysis and mass spectral analysis. Our results showed that the molluscicidal activity of the root crude extract differed between P. canaliculata with different shell diameters after treatment for 72 h. The median lethal concentration (LC50 ) was 5.511 mg L(-1) against snails of 1.5 ± 0.2 cm diameter and 12.383 mg L(-1) against snails of 2.5 ± 0.2 cm diameter. Furthermore, two active ingredients isolated from root methanol extracts were identified as pedunsaponin A and pedunsaponin C. Both pedunsaponin A and pedunsaponin C showed strong molluscicidal activities, with LC50 values of 3.893 and 4.252 mg L(-1) , respectively, against snails with shell diameters of 1.5 ± 0.2 cm after treatment for 72 h. Pueraria peduncularis extracts exhibit high molluscicidal activity and have great potential value for exploring a molluscicide to control Pomacea canaliculata. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  1. Tectoridin, an isoflavone glycoside from the flower of Pueraria lobata, prevents acute ethanol-induced liver steatosis in mice.

    PubMed

    Xiong, Yu; Yang, Yuqing; Yang, Jin; Chai, Hongyan; Li, Ying; Yang, Jing; Jia, Ziming; Wang, Zhengrong

    2010-09-30

    In traditional Chinese medicine, the flower of Pueraria lobata (Puerariae Flos) has been used in therapy to counteract the problems associated with alcohol drinking and liver injury. In this study, we investigated the hepatoprotective effects and its mechanisms of tectoridin, an isoflavone glycoside from the flower of P. lobata (Willd.) Ohwi. Ethanol (5g/kg) was given orally every 12h for a total of three doses. 1h after the last dose of ethanol, tectoridin (25, 50 and 100mg/kg) was given intragastrically five times in three consecutive days. The mice were sacrificed at 4h after tectoridin treatment. Peroxisome proliferators-activated receptor alpha (PPARalpha), sterol regulatory element-binding protein (SREBP)-1c and their target genes were evaluated by biochemical analysis and quantitative real-time polymerase chain reaction (qPCR). Mitochondria were isolated for the mitochondrial permeability transition (MPT) and membrane potential (DeltaPsi(m)) assay. Acute ethanol exposure resulted in the significant increase of the alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG) levels and hepatic mitochondria dysfunction shown as the increase of MPT and the decrease of DeltaPsi(m). However, tectoridin treatment dramatically attenuated these effects. In addition, tectoridin remarkably alleviated the over-production of thiobarbituric acid-reactive substance. Furthermore, tectoridin inhibited the decrease of PPARalpha expression and its target genes, including medium-chain acyl-CoA dehydrogenase (MCAD), acyl-CoA oxidase (ACO) and cytochrome P450 4A (CYP 4A) at mRNA and enzyme activity levels. These data showed that tectoridin protected against ethanol-induced liver steatosis mainly through modulating the disturbance of PPARalpha pathway and ameliorating mitochondrial function. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.

  2. 30 CFR 926.15 - Approval of Montana regulatory program amendments.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 3 2012-07-01 2012-07-01 false Approval of Montana regulatory program..., DEPARTMENT OF THE INTERIOR PROGRAMS FOR THE CONDUCT OF SURFACE MINING OPERATIONS WITHIN EACH STATE MONTANA § 926.15 Approval of Montana regulatory program amendments. The following is a list of the...

  3. 30 CFR 926.15 - Approval of Montana regulatory program amendments.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 3 2013-07-01 2013-07-01 false Approval of Montana regulatory program..., DEPARTMENT OF THE INTERIOR PROGRAMS FOR THE CONDUCT OF SURFACE MINING OPERATIONS WITHIN EACH STATE MONTANA § 926.15 Approval of Montana regulatory program amendments. The following is a list of the...

  4. 76 FR 37372 - Notice of Proposed Withdrawal Extension and Notification of a Public Meeting; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-27

    ...; Montana AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: The U.S. Fish and Wildlife... Wildlife Refuge (CMR NWR). The surface is protected by an overlapping withdrawal. This notice gives an... the Montana State Director, Bureau of Land Management, 5001 Southgate Drive, Billings, Montana...

  5. 30 CFR 926.15 - Approval of Montana regulatory program amendments.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 3 2014-07-01 2014-07-01 false Approval of Montana regulatory program..., DEPARTMENT OF THE INTERIOR PROGRAMS FOR THE CONDUCT OF SURFACE MINING OPERATIONS WITHIN EACH STATE MONTANA § 926.15 Approval of Montana regulatory program amendments. The following is a list of the...

  6. 30 CFR 926.15 - Approval of Montana regulatory program amendments.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 3 2011-07-01 2011-07-01 false Approval of Montana regulatory program..., DEPARTMENT OF THE INTERIOR PROGRAMS FOR THE CONDUCT OF SURFACE MINING OPERATIONS WITHIN EACH STATE MONTANA § 926.15 Approval of Montana regulatory program amendments. The following is a list of the...

  7. 76 FR 46829 - Montana; Amendment No. 1 to Notice of a Major Disaster Declaration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... SECURITY Federal Emergency Management Agency Montana; Amendment No. 1 to Notice of a Major Disaster... notice of a major disaster declaration for the State of Montana (FEMA-1996-DR), dated June 17, 2011, and... for the State of Montana is hereby amended to include the following areas among those areas determined...

  8. 76 FR 47221 - Montana; Amendment No. 3 to Notice of a Major Disaster Declaration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-04

    ... SECURITY Federal Emergency Management Agency Montana; Amendment No. 3 to Notice of a Major Disaster... notice of a major disaster declaration for the State of Montana (FEMA-1996-DR), dated June 17, 2011, and... for the State of Montana is hereby amended to include the Individual Assistance program for the...

  9. 30 CFR 926.20 - Approval of Montana abandoned mine land reclamation plan.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 3 2013-07-01 2013-07-01 false Approval of Montana abandoned mine land reclamation plan. 926.20 Section 926.20 Mineral Resources OFFICE OF SURFACE MINING RECLAMATION AND ENFORCEMENT... § 926.20 Approval of Montana abandoned mine land reclamation plan. The Montana Abandoned Mine Land...

  10. 76 FR 28065 - Notice of Intent To Repatriate a Cultural Item: Montana Historical Society, Helena, MT

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-13

    ... National Park Service Notice of Intent To Repatriate a Cultural Item: Montana Historical Society, Helena... repatriate a cultural item in the possession of the Montana Historical Society, Helena, MT, that meets the... Territory, between 1884 and 1886. In 1892, Allen loaned it to the Montana Historical Society....

  11. 75 FR 58430 - Notice of Inventory Completion: University of Montana, Missoula, MT

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-24

    ... National Park Service Notice of Inventory Completion: University of Montana, Missoula, MT AGENCY: National... human remains and an associated funerary object in the possession of the University of Montana, Missoula... determinations in this notice. A detailed assessment of the human remains was made by University of Montana...

  12. 78 FR 49510 - Cross-Media Electronic Reporting: Authorized Program Revision Approval, State of Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-14

    ... State of Montana's request to revise its National Primary Drinking Water Regulations Implementation EPA... the State of Montana's National Primary Drinking Water Regulations Implementation program, if no... Montana Department of Environmental Quality (MT DEQ) submitted an application titled ``Safe Drinking Water...

  13. 78 FR 53158 - Notice of Public Meeting; Central Montana Resource Advisory Council

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-28

    ... field trip; 9th District Court Decisions; Monument Leases Vacated; Sage Grouse and Lewistown RMP update... Montana District Office, Lewistown Field Office Conference room at 920 NE Main, Lewistown, Montana. FOR FURTHER INFORMATION CONTACT: Gary L. ``Stan'' Benes, Central Montana District Manager, Lewistown Field...

  14. Montana Standards and Guidelines for Career and Vocational/Technical Education. Summer 2002 Update.

    ERIC Educational Resources Information Center

    Montana State Office of Public Instruction, Helena.

    This document presents Montana's standards and guidelines for career and vocational/technical education (CVTE). The guide begins with a directory of Montana's key CVTE personnel and specialists. Part 1 presents the federal and state definitions of CVTE and provides an overview of Montana's system for delivering CVTE and its philosophy regarding…

  15. 76 FR 69281 - Notice of Public Meeting; Central Montana Resource Advisory Council

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-08

    ... Bureau of Land Management Notice of Public Meeting; Central Montana Resource Advisory Council AGENCY... of the Interior, Bureau of Land Management (BLM) Central Montana Resource Advisory Council (RAC) will...: The meetings will be in the Bureau of Land Management's Central Montana District Office, at 920 NE...

  16. 77 FR 46109 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-02

    ..., Billings, Montana, on September 4, 2012. DATES: Protests of the survey must be filed before September 4... Survey, Bureau of Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER..., 5001 Southgate Drive, Billings, Montana 59101-4669, telephone (406) 896-5124 or (406) 896-5009,...

  17. 76 FR 9049 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-16

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana..., 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Josh Alexander, Cadastral Surveyor, Branch of Cadastral Survey, Bureau of Land Management, 5001 Southgate Drive,...

  18. 75 FR 31812 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-04

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Survey, Bureau of Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669, telephone (406...: May 27, 2010. James D. Claflin, Chief Cadastral Surveyor, Division of Resources. BILLING CODE...

  19. 76 FR 37372 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-27

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Management, 5001 Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Steve Toth, Cadastral Surveyor, Branch of Cadastral Survey, Bureau of Land Management, 5001 Southgate Drive,...

  20. 75 FR 41881 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Drive, Billings, Montana 59101-4669, telephone (406) 896-5121 or (406) 896- 5009. SUPPLEMENTARY..., Division of Resources. BILLING CODE 4310-DN-P...

  1. 77 FR 46109 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-02

    ... survey of the lands described below in the BLM Montana State Office, Billings, Montana, on September 4... Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin Montoya, Cadastral Surveyor, Branch of Cadastral Survey, Bureau of Land Management, 5001 Southgate Drive, Billings,...

  2. 75 FR 48722 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-11

    ... file the plat of survey of the lands described below in the BLM Montana State Office, Billings, Montana... Survey, Bureau of Land Management, 5001 Southgate Drive, Billings, Montana 59101-4669, telephone (406...: August 4, 2010. James D. Claflin, Chief Cadastral Surveyor, Division of Resources. BILLING CODE 4310-DN-P...

  3. 76 FR 44947 - Notice of Filing of Plats of Survey; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-27

    ... survey of the lands described below in the BLM Montana State Office, Billings, Montana, on August 26... Southgate Drive, Billings, Montana 59101-4669. FOR FURTHER INFORMATION CONTACT: Marvin Montoya, Cadastral Surveyor, Branch of Cadastral Survey, Bureau of Land Management, 5001 Southgate Drive, Billings,...

  4. Occurrences of the western skink (Eumeces skiltonianus) in grasslands of western Montana

    Treesearch

    Yvette K. Ortega; Dean E. Pearson

    2001-01-01

    The western skink (Eumeces skiltonianus) was recently classified as a Montana Species of Special Concern due to its restricted range in the state (D. P. Hendricks, Montana Natural Heritage Program, Helena, MT, pers. comm.). Records of the western skink in Montana are limited to a few, scattered locations on the western edge of the state (Tanner 1988; Reichel...

  5. Annual Report to the Board of Regents of the Montana University System, 1973-74.

    ERIC Educational Resources Information Center

    Montana Univ. System, Helena.

    This is the first annual report to the Board of Regents from the Commissioner of Higher Education. The purpose of the report is to provide the Board of Regents with comprehensive information on programs and policies of the six institutions of the Montana University System: University of Montana at Missoula, Montana State University at Bozeman,…

  6. Montana School Fall Enrollment by Race/Ethnicity, 2003-04 School Year

    ERIC Educational Resources Information Center

    McCulloch, Linda

    2004-01-01

    This report discusses school fall Enrollment of Montana based on race/ethnicity for the 2003-2004 school year. Sections include: (1) Montana School Fall Enrollment by Race/Ethnicity Overview; and (2) Montana School Fall Enrollment by Race/Ethnicity-2003-04 School Year. Fall enrollment data are collected by school, grade, gender, and race/ethnicity…

  7. Holocene vegetational history of the Kootenai River Valley, Montana

    NASA Astrophysics Data System (ADS)

    Mack, Richard N.; Rutter, N. W.; Valastro, S.

    1983-09-01

    Pollen records in the Kootenai and Fisher River drainages in western Montana reveal a fivezone sequence of Holocene vegetation change. Deposition of Glacier Peak Ash-Layer G (ca. 10,540 ± 660 yr B.P.) in the lowermost sediments (clay intermixed with pebbles) at Tepee Lake gives a minimum date for the initiation of sedimentation. Initial vegetation on the newly deglaciated terrain was dominated by Pinus (probably white bark pine) with small amounts of Gramineae, Picea and Abies, reflecting a relatively cool, moist macroclimate. Two vegetation units appear to contribute to Pollen Zone II (ca. 11,000-7100 yr B.P.): arboreal communities with pines, along with Pseudotsuga or Larix, or both, and treeless vegetation dominated by Artemisia. Pollen Zone II represents an overall warmer macroclimate than occurred upon ice withdrawal. After ca. 7100 yr B.P. (Pollen Zone III) diploxylon pines became a major pollen contributor near both Tepee Lake and McKillop Creek Pond, indicating an expansion of xerophytic forest ( P. contorta and P. ponderosa) along with an increase in the prominence of Pseudotsuga menziesii or Larix occidentalis, or both. Artemisia briefly expanded coverage near Tepee Lake concomitant with the Mazama ashfall ca. 6700 yr B.P. A short-term climatic trend with more available water began after ca. 4000 yr B.P. as Abies (probably A. grandis) along with Picea engelmannii became a more regular component of the forest surrounding both sites. Emergence of the modern macroclimate is indicated primarily with the first regular appearance of Tsuga heterophylla in the pollen record by ca. 2700 yr B.P., synchronous with the development of western hemlock forest within the same latitudes in northern Idaho and northeastern Washington.

  8. Heat pump systems for Spring Creek, Montana

    SciTech Connect

    Engen, I.A.

    1982-03-01

    The use of ground water heat pump systems for space heating in the new town of Spring Creek, Montana, is reviewed in this report. The available information, together with a review of manufacturers' specifications and guidelines, indicates ground water heat pump systems can be competitive with comparable electric space conditioning systems, if electricity cost approaches $0.02/kWh. Due to the low water temperature, large volumes of water will be required to carry the peak heat load and district-type supply systems may not be feasible for single-family residential developments. Due to the large water production rates, shallow depth of the reservoir, and proximity of a large surface reservoir, additional reservoir evaluation seems appropriate; obtaining competent hydrological consultation is recommended. If ground water heat pump systems are used in the development care must be exercised in equipment selection; the requirement for cooling capacity at the site is negligible compared to heating load. Some heat pumps designed for southern climates may not provide adequate heating performance on water below 60/sup 0/F.

  9. Archean collisional tectonics in SW Montana

    SciTech Connect

    Mogk, D.; Rickmond, D.; Salt, K.; Clark, M.; Mueller, P.; Lafrenze, D.; Wooden, J.; Henry, D.

    1985-01-01

    The Archean continental crust of SW Montana evolved through alternating cycles of stable platform sedimentation followed by crustal thickening through collisional tectonics. The ancient sialic crust in the Beartooth Mountains served as the nucleus for accretion of younger terranes to the west. The oldest orogenic cycle recognized in the Beartooth Mountains involves a 3.4 Ga old supracrustal sequence which was metamorphosed in the granulite facies (T=700-800/sup 0/C, P=6Kb, 35/sup 0/C/Km); deep burial is interpreted as the result of collisional tectonic thickening. The second orogenic cycle is subduction related and has produced 2.8 Ga old andesites, 2.75 Ga old calc-alkaline intrusives, upper amphibolite grade metamorphism, transcurrent faulting (in the North Snowy Block and Yankee Jim canyon at 2.8 Ga) and nappe emplacement. In the central Beartooths post-orogenic granites intrude pelitic schists (T=600/sup 0/C, P=8Kb, 25/sup 0/C/Km). West of the Beartooths the basement consists of 2.75-2.70 Ga old, tectonically telescoped coarse clastics (Gallatin, Madison Ranges) and stable platform sequences (Gravelly, Tobacco Root, Ruby Ranges). Nappe formation and granulite-migmatite (700-750/sup 0/C) associations are common, suggesting deep burial through tectonic thickening. A later-kinematic mesozonal (8Kb) qtz diorite-granodiorite batholithic complex is present in the northern Madison Range. Quartzofeldspathic paragneisses in the westernmost Archean basement are derived from either a continental or island arc source.

  10. National Uranium Resource Evaluation: Bozeman Quadrangle, Montana

    SciTech Connect

    Lange, I.M.; Fields, R.W.; Fountain, D.M.; Moore, J.N.; Qamar, A.I.; Silverman, A.J.; Thompson, G.R.; Chadwick, R.A.; Custer, S.G.; Smith, D.L.

    1982-08-01

    The Bozeman Quadrangle, Montana, was evaluated to identify and delineate areas containing environments favorable for uranium deposits. This evaluation was conducted using methods and criteria developed for the National Uranium Resource Evaluation program. General surface reconnaissance, mapping, radiometric traverses, and geochemical sampling were performed in all geologic environments within the quadrangle. Aerial radiometric and HSSR data were evaluated and followup studies of these anomalies and most of the previously known uranium occurrences were conducted. Detailed gravity profiling was done in the Tertiary Three Forks-Gallatin Basin and the Madison and Paradise Valleys. Also, selected well waters were analyzed. Eight areas are considered favorable for sandstone uranium deposits. They include the Tertiary Three Forks-Gallatin basin, the Madison and Paradise Valleys, and five areas underlain by Cretaceous fluvial and marginal-marine sandstones. Other environments within the quadrangle are considered unfavorable for uranium deposits when judged by the program criteria. A few environments were not evaluated due to inaccessibility and/or prior knowledge of unfavorable criteria.

  11. The Montana earthquake of June 27, 1925

    USGS Publications Warehouse

    Pardee, J.T.

    1927-01-01

    The earthquake of June 27, 1925, in Montana caused considerable damage within an area of 600 square miles or more, the center of which is in latitude 46° 5' N. and longitude 111° 20' W., a short distance southeast of Lombard. It was a seismic disturbance of the first order of magnitude, but, owing to the hour at which it occurred and to other fortunate circumstances, no lives were lost and no fires broke out. The shock was startling throughout an area extending 75 miles or more in all directions from the epicenter and was sensible to persons within an area of 310,000 square miles. Within the epicentral area brick buildings suffered severely, rocks fell from cliffs, cracks opened in the ground, and the inhabitants experienced the usual symptoms of illness and emotions of alarm. Isoseismals drawn according to the Rossi-Forel scale show a wide indentation at the south due to a rapid decline of intensity in the volcanic area of Snake River Plain and Yellowstone Park.

  12. Anti-Endometriotic Effects of Pueraria Flower Extract  in Human Endometriotic Cells and Mice.

    PubMed

    Kim, Ji-Hyun; Woo, Jeong-Hwa; Kim, Hye Mi; Oh, Myung Sook; Jang, Dae Sik; Choi, Jung-Hye

    2017-02-28

    Pueraria flowers have been used as a vegetable and an ingredient for tea and jelly. In this study, we investigated the effects of Pueraria flower extract (PFE) on endometriosis, a common gynaecological disease characterised by local sterile inflammation of peritoneal cavity. PFE suppressed the adhesion of human endometriotic cells 11Z and 12Z to human mesothelial Met5A cells. In addition, PFE significantly inhibited the migration of 11Z and 12Z cells as shown by woundhealing and transwell migration assays. PFE reduced the protein and mRNA levels of matrix metalloproteinase (MMP)-2 and MMP-9 in endometriotic cells. Moreover, extracellular signalregulated kinase (ERK)1/2 was activated by PFE treatment, and an ERK1/2 inhibitor, PD98059, significantly inhibited PFE-inhibited cell migration in endometriotic cells. Furthermore, PFE significantly suppressed endometriotic lesion formation in a mouse model. These data suggest that Pueraria flower is a potential anti-endometriotic agent for the inhibition of endometriotic cell adhesion, migration, and MMP expression.

  13. Rapid simultaneous determination of isoflavones in Radix puerariae using high-performance liquid chromatography-triple quadrupole mass spectrometry with novel shell-type column.

    PubMed

    Du, Gang; Zhao, Haiyu; Song, Yuelin; Zhang, Qingwen; Wang, Yitao

    2011-10-01

    A high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry (MS/MS) method was developed for rapid determination of 13 isoflavones in Radix puerariae. A novel shell-type column, namely Kinetex core-shell C(18) column (50 mm×2.1 mm id, 2.6 μm), and gradient elution were used during the analysis. The chromatographic peaks of 13 investigated compounds were identified by comparing their retention time and MS data with the related reference compounds. Multiple-reaction monitoring (MRM) was employed for the quantitative analysis with negative ionization mode. All calibration curves showed good linearity (r(2)>0.9990) within test ranges. The LOD and LOQ were lower than 0.017 and 0.873 μg/mL on column, respectively. The intra- and inter-day precisions for 13 analytes were <1.17 and 2.17%, respectively, and the recoveries were 93.1-104.4%. The validated method was applied for quantitative analysis of 13 isoflavones in 7 species of Radix puerariae. The result demonstrated that HPLC-MS/MS system with Kinetex column could be a promising analytical tool for the determination of isoflavones in traditional Chinese medicines, which is helpful for comprehensive evaluation of quality of R. puerariae. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Simultaneous determination of isoflavones, saponins and flavones in Flos Puerariae by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.

    PubMed

    Lu, Jing; Xie, Yuanyuan; Tan, Yao; Qu, Jialin; Matsuda, Hisashi; Yoshikawa, Masayuki; Yuan, Dan

    2013-01-01

    An ultra performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (QTOF/MS) method is established for the rapid analysis of isoflavones, saponins and flavones in 16 samples originated from the flowers of Pueraria lobata and P. thomsonii. A total of 25 isoflavones, 13 saponins and 3 flavones were identified by co-chromatography of sample extract with authentic standards and comparing the retention time, UV spectra, characteristic molecular ions and fragment ions with those of authentic standards, or tentatively identified by MS/MS determination along with Mass Fragment software. Moreover, the method was validated for the simultaneous quantification of 29 components. The samples from two Pueraria flowers significantly differed in the quality and quantity of isoflavones, saponins and flavones, which allows the possibility of showing their chemical distinctness, and may be useful in their standardization and quality control. Dataset obtained from UPLC-MS was processed with principal component analysis (PCA) and orthogonal partial least squared discriminant analysis (OPLS-DA) to holistically compare the difference between both Pueraria flowers.

  15. Karyomorphometric analysis of Fritillaria montana group in Greece

    PubMed Central

    Samaropoulou, Sofia; Bareka, Pepy; Kamari, Georgia

    2016-01-01

    Abstract Fritillaria Linnaeus, 1753 (Liliaceae) is a genus of geophytes, represented in Greece by 29 taxa. Most of the Greek species are endemic to the country and/or threatened. Although their classical cytotaxonomic studies have already been presented, no karyomorphometric analysis has ever been given. In the present study, the cytological results of Fritillaria montana Hoppe ex W.D.J. Koch, 1832 group, which includes Fritillaria epirotica Turrill ex Rix, 1975 and Fritillaria montana are statistically evaluated for the first time. Further indices about interchromosomal and intrachromosomal asymmetry are given. A new population of Fritillaria epirotica is also investigated, while for Fritillaria montana, a diploid individual was found in a known as triploid population. Paired t-tests and PCoA analysis have been applied to compare the two species. PMID:28123688

  16. Assessment of Autoimmune Responses Associated with Asbestos Exposure in Libby, Montana, USA

    PubMed Central

    Pfau, Jean C.; Sentissi, Jami J.; Weller, Greg; Putnam, Elizabeth A.

    2005-01-01

    Systemic autoimmune responses are associated with certain environmental exposures, including crystalline particles such as silica. Positive antinuclear antibody (ANA) tests have been reported in small cohorts exposed to asbestos, but many questions remain regarding the prevalence, pattern, and significance of autoantibodies associated with asbestos exposures. The population in Libby, Montana, provides a unique opportunity for such a study because of both occupational and environmental exposures that have occurred as a result of the mining of asbestos-contaminated vermiculite near the community. As part of a multifaceted assessment of the impact of asbestos exposures on this population, this study explored the possibility of exacerbated autoimmune responses. Age- and sex-matched sets of 50 serum samples from Libby and Missoula, Montana (unexposed), were tested for ANA on HEp-2 cells using indirect immunofluorescence. Data included frequency of positive tests, ANA titers, staining patterns, and scored fluorescence intensity, all against known controls. Serum immunoglobulin A (IgA), rheumatoid factor, and antibodies to extractable nuclear antigen (ENA) were also tested. The Libby samples showed significantly higher frequency of positive ANA and ENA tests, increased mean fluorescence intensity and titers of the ANAs, and higher serum IgA, compared with Missoula samples. In the Libby samples, positive correlations were found between ANA titers and both lung disease severity and extent of exposure. The results support the hypothesis that asbestos exposure is associated with autoimmune responses and suggests that a relationship exists between those responses and asbestos-related disease processes. PMID:15626643

  17. Characterization and evaluation of smoke tracers in PM: Results from the 2003 Montana wildfire season

    NASA Astrophysics Data System (ADS)

    Ward, Tony J.; Hamilton, Raymond F.; Dixon, Roy W.; Paulsen, Michael; Simpson, Christopher D.

    Throughout August and September 2003, Missoula, Montana was heavily impacted by smoke from wildland forest fires burning throughout western Montana. In an effort to study the organic compounds contributing to smoke particulate matter impacting downwind communities, three co-located PM 2.5 cyclones collected 24-h samples during significant smoke events in Missoula. Filter samples were then analyzed at two independent laboratories to quantify the concentrations of several chemical markers of wood smoke generated under natural combustion conditions, as well as to provide an intercomparison study of analytical methods (HPLC and GC/MS) used in the determination of levoglucosan concentrations. Concentrations of monosaccharide anhydrides, methoxyphenols and soluble potassium were measured from PM 2.5 samples collected during periods of smoke and baseline (non-impacted) conditions. From these analyses, the associations between the wood smoke markers and PM 2.5 mass were examined. Levoglucosan was found to be the most useful marker for wood smoke generated from natural forest fire events in the northern Rocky Mountains, whereas measurement of other species (mannosan, methoxyphenols) provided information on the type of vegetative material burned (e.g., hardwood versus softwood). The emission ratios of levoglucosan to PM 2.5 and organic carbon from the wildfire smoke was found to be 0.042 and 0.062, respectively. Results of the statistical analyses showed that the GC/MS and HPLC approaches to levoglucosan analysis were statistically equivalent.

  18. Perioperative Arnica montana for Reduction of Ecchymosis in Rhinoplasty Surgery.

    PubMed

    Chaiet, Scott R; Marcus, Benjamin C

    2016-05-01

    Studies of homeopathic therapies to decrease postrhinoplasty ecchymosis have previously used subjective measurements, limiting their clinical significance. Recently, Arnica montana was shown to decrease postoperative ecchymosis after rhytidectomy, using an objective measuring tool. We believe that oral A. montana, given perioperatively, can be objectively shown to reduce extent and intensity of postoperative ecchymosis in rhinoplasty surgery. Subjects scheduled for rhinoplasty surgery with nasal bone osteotomies by a single surgeon were prospectively randomized to receive either oral perioperative A. montana (Alpine Pharmaceuticals, San Rafael, Calif) or placebo in a double-blinded fashion. Ecchymosis was measured in digital "three-quarter"-view photographs at 3 postoperative time points. Each bruise was outlined with Adobe Photoshop (Adobe Systems Incorporated, San Jose, Calif), and the extent was scaled to a standardized reference card. Cyan, magenta, yellow, black, and luminosity were analyzed in the bruised and control areas to calculate change in intensity. P value of <0.1 was set as a meaningful difference with statistical significance. Compared with 13 subjects receiving placebo, 9 taking A. montana had 16.2%, 32.9%, and 20.4% less extent on postoperative days 2/3, 7, and 9/10, a statistically significant difference on day 7 (P = 0.097). Color change initially showed 13.1% increase in intensity with A. montana but 10.9% and 36.3% decreases on days 7 and 9/10, a statistically significant difference on day 9/10 (P = 0.074). One subject experienced mild itching and rash with the study drug that resolved during the study period. Arnica montana seems to accelerate postoperative healing, with quicker resolution of the extent and the intensity of ecchymosis after osteotomies in rhinoplasty surgery, which may dramatically affect patient satisfaction.

  19. Fascioloidiasis in game-ranched elk from Montana.

    PubMed

    Hood, B R; Rognlie, M C; Knapp, S E

    1997-10-01

    The distribution of Fascioloides magna in game-ranched elk and the potential for spread of the parasite through movement of infected animals was examined in Montana (USA). Fecal samples (n = 448) collected from captive elk on 29 game ranches were examined for eggs of F. magna by fecal sedimentation. Eggs were detected in elk on 5 ranches. This suggests that F. magna has been translocated by infected game-ranched elk. The wide distribution of snail intermediate hosts for F. magna in Montana indicates a potential to spread the parasite to other captive cervids domestic livestock or free-ranging wildlife.

  20. Methods for estimating streamflow characteristics at ungaged sites in western Montana based on data through water year 2009: Chapter G in Montana StreamStats

    USGS Publications Warehouse

    McCarthy, Peter M.; Sando, Roy; Sando, Steven K.; Dutton, DeAnn M.

    2016-04-05

    All of the data used to calculate basin characteristics were derived from publicly available data sources and are available through the U.S. Geological Survey Streamstats program (http://water.usgs.gov/osw/streamstats/) for Montana. The primary purpose of the Montana StreamStats application is to provide estimates of basin characteristics and streamflow characteristics for user-selected ungaged sites on Montana streams. The regional regression equations presented in this report have been loaded to the Montana StreamStats application and can be used to derive streamflow characteristics for ungaged sites.

  1. Utilizing Paraeducators as Links to Their Communities: A Training Package for Use with Teacher/Paraeducator Teams and in the Pre-Service Training of Teachers.

    ERIC Educational Resources Information Center

    Hermanson, Michael J.; Peterson, Michael D.; Sampson, Marsha; Hoagland, Tina

    To deliver training and continuing education for education personnel in a large rural state, Montana has decentralized its Comprehensive System of Personnel Development (CSPD) by forming five regional councils. The Montana Center on Disabilities developed a statewide training program for using paraeducators as liaisons to communities to enhance…

  2. Bacterial Communities of Bartonella-Positive Fleas: Diversity and Community Assembly Patterns▿

    PubMed Central

    Jones, Ryan T.; McCormick, Katherine F.; Martin, Andrew P.

    2008-01-01

    We investigated the bacterial communities of nine Bartonella-positive fleas (n = 6 Oropsylla hirsuta fleas and n = 3 Oropsylla montana fleas), using universal primers, clone libraries, and DNA sequencing. DNA sequences were used to classify bacteria detected in a phylogenetic context, to explore community assembly patterns within individual fleas, and to survey diversity patterns in dominant lineages. PMID:18203862

  3. A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry.

    PubMed

    Du, G; Zhao, H Y; Zhang, Q W; Li, G H; Yang, F Q; Wang, Y; Li, Y C; Wang, Y T

    2010-01-29

    A microwave-assisted extraction (MAE) and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry (UHPLC-DAD-TOF-MS) method was developed for simultaneous determination of 14 phenolic compounds in the root of Pueraria lobata (Wild.) Ohwi and Pueraria thomsonii Benth. Operational conditions of MAE were optimized by central composite design (CCD). The optimized result was 65% ethanol as extraction solvent, 17mL of extraction volume, 100 degrees C of extraction temperature and 2min of hold time. A Zorbax SB C(18) (50mmx4.6mm I.D., 1.8microm) and gradient elution were used during the analysis. The chromatographic peaks of 14 investigated compounds in samples were successfully identified by comparing their retention time, UV spectra and TOF mass data with the reference substances. All calibration curves showed good linearity (r>0.9997) within the test ranges. The intra-day and inter-day variations were less than 1.77% and 2.88%, respectively. The developed method was successfully applied to determine the investigated compounds in 10 samples of Radix Puerariae Lobatae and Radix Puerariae Thomsonii, respectively. The result indicated that MAE and UHPLC-DAD-TOF-MS system might provide a rapid method for the quality control of Radix Puerariae.

  4. Feasibility Study of Biopower in East Helena, Montana. A Study Prepared in Partnership with the Environmental Protection Agency for the RE-Powering America's Land Initiative: Siting Renewable Energy on Potentially Contaminated Land and Mine Sites

    SciTech Connect

    Moriarty, K.

    2013-02-01

    The U.S. Environmental Protection Agency (EPA) developed the RE-Powering America's Land initiative to reuse contaminated sites for renewable energy generation when aligned with the community's vision for the site. The former American Smelting and Refining Company (Asarco) smelter in East Helena, Montana, was selected for a feasibility study under the initiative. Biomass was chosen as the renewable energy resource based on the wood products industry in the area. Biopower was selected as the technology based on Montana's renewable portfolio standard (RPS) requiring utilities to purchase renewable power.

  5. Community.

    ERIC Educational Resources Information Center

    Grauer, Kit, Ed.

    1995-01-01

    Art in context of community is the theme of this newsletter. The theme is introduced in an editorial "Community-Enlarging the Definition" (Kit Grauer). Related articles include: (1) "The Children's Bridge is not Destroyed: Heart in the Middle of the World" (Emil Robert Tanay); (2) "Making Bridges: The Sock Doll…

  6. Regional assemblages of Lygus (Heteroptera: Miridae) in Montana canola fields

    USDA-ARS?s Scientific Manuscript database

    Sweep net sampling of canola (Brassica napus L.) was conducted in 2002 and 2003 to determine Lygus (Heteroptera: Miridae) species composition and parasitism levels in four regions of Montana. Regardless of region or seasonal change, Lygus elisus (Van Duzee) was the dominant species in all canola fi...

  7. Hydrologic and geologic characterization of Tenderfoot Creek Experimental Forest, Montana

    Treesearch

    Phillip E. Farnes; Ward W. McCaughey; Katherine J. Hansen

    1994-01-01

    Tenderfoot Creek Experimental Forest (TCEF) is located in Central Montana 24 miles north of White Sulphur Springs and 9 miles northwest of Highway 89 from Kings Hill via Forest Road #839. The experimental forest can also be accessed by Forest Road #586 via Sheep Creek. A general view of TCEF showing roads and drainages is shown in figure 2. The road down Tenderfoot...

  8. Ecological adaptations in Douglas-fir populations. II. Western Montana

    Treesearch

    Gerald E. Rehfeldt

    1982-01-01

    Populations of Douglas-fir (Pseudotsuga menziesii var. glauca) from Montana west of the Continental Divide were compared in common environments. Differentiation was observed for six variables reflecting growth potential, phenology and cold hardiness. Adaptation of populations for numerous traits is viewed as a balance between selection of high growth potential in...

  9. Geology of the Limestone Hills, Broadwater County, Montana

    USGS Publications Warehouse

    Ruppel, Edward Thompson

    1950-01-01

    The Limestone Hills, a natural topographic unit having an area of about 16 square miles, are situated a few miles west and southwest of Townsend, Broadwater County, Montana. They are composed of pre-Cambrian, Paleozoic, and Mesozoic sedimentary rocks i

  10. The Bitterroot Valley of western Montana: Area economic profile

    Treesearch

    Larry Swanson

    2001-01-01

    This profile provides a description and assessment of the area economy of the Bitterroot Valley of southwestern Montana. Changing conditions and trends in the area economy over the course of the last twenty to twenty- five years are examined, including trends in population and employment growth and associated income changes and economic restructuring. Changing...

  11. National Environmental/Energy Workforce Assessment for Montana.

    ERIC Educational Resources Information Center

    National Field Research Center Inc., Iowa City, IA.

    This report presents existing workforce levels, training programs and career potentials and develops staffing level projections (1976-1982) based on available information for the State of Montana. The study concerns itself with the environmental pollution control areas of air, noise, potable water, pesticides, radiation, solid waste, wastewater,…

  12. Race, Hunger, and Poverty on Montana Indian Reservations.

    ERIC Educational Resources Information Center

    Brod, Rodney L.; Miller, Paul E.

    1998-01-01

    Uses a quantitative analytic procedure, logistic regression, to search for and identify critical attributes of race, highlighting the characteristics of American Indians that underlie poverty on Montana's seven reservations, with their varied tribal affiliations. Implications are drawn for the Food Distribution Program on Indian Reservations,…

  13. Montana Kids Count Data Book and County Profiles, 1995.

    ERIC Educational Resources Information Center

    Healthy Mothers, Healthy Babies--The Montana Coalition, Helena.

    This Kids Count report is the second to examine statewide trends in the well-being of Montana's children. The statistical portrait is based on 16 indicators of well-being: (1) low birthweight; (2) infant mortality; (3) child death; (4) teen violent death; (5) Medicaid participation; (6) school enrollment; (7) public school enrollment in Chapter 1…

  14. Montana Kids Count Data Book and County Profiles, 1994.

    ERIC Educational Resources Information Center

    Healthy Mothers, Healthy Babies--The Montana Coalition, Helena.

    This Kids Count publication is the first to examine statewide trends in the well-being of Montana's children. The statistical portrait is based on 13 indicators of well-being: (1) low birthweight rate; (2) infant mortality rate; (3) child death rate; (4) teen violent death rate; (5) percent of public school enrollment in Chapter 1 programs; (6)…

  15. Growing media trials at the Montana Conservation Seedling Nursery

    Treesearch

    John Justin

    2009-01-01

    The Montana Conservation Seedling Nursery (MCSN) in Missoula produces 750,000 container seedlings annually in containers ranging in size from 66 cm3 (4 in3) up to 61 L (16 gal) pots. The MCSN is a production facility with no research funding. When we encounter a promising idea for improving our seedlings or the efficiency of nursery operations, we rarely perform...

  16. The effect of disturbance history on hawkweed invasion (Montana)

    Treesearch

    Alexis Jones; Elizabeth Crone

    2009-01-01

    Orange hawkweed (Hieracium aurantiacum) is listed as a noxious weed in five states (USDA 2007), including Montana, where it is still in the early - and possibly controllable - stages of invasion. The species forms dense clonal mats that exclude natives from the area; moreover, the wind-borne seeds are viable in the soil for seven years and have a...

  17. Race, Hunger, and Poverty on Montana Indian Reservations.

    ERIC Educational Resources Information Center

    Brod, Rodney L.; Miller, Paul E.

    1998-01-01

    Uses a quantitative analytic procedure, logistic regression, to search for and identify critical attributes of race, highlighting the characteristics of American Indians that underlie poverty on Montana's seven reservations, with their varied tribal affiliations. Implications are drawn for the Food Distribution Program on Indian Reservations,…

  18. Montana Workforce to the Year 2000: Opportunities and Challenges.

    ERIC Educational Resources Information Center

    Montana State Dept. of Labor and Industry, Helena.

    Beginning with a glance at Montana's past, this publication looks to the state's future in terms of demographic patterns, employment growth, technological changes, educational needs, and workplace changes. Population, work force, and employment projections are compared with national trends. Productivity and job changing are considered, and…

  19. Lumber recovery from ponderosa pine in western Montana.

    Treesearch

    Marlin E. Plank

    1982-01-01

    Lumber grade yields and recovery ratios are shown for a sample of ponderosa pine (Pinus ponderosa Dougl. ex Laws.) in western Montana. About 9 percent of the lumber produced was in Select grades, 48 percent in Shop grades, and 43 percent in Common grades. Information on log scale and yield is presented in tables by log grade and diameter class....

  20. The Condition of College & Career Readiness 2016: Montana Key Findings

    ERIC Educational Resources Information Center

    ACT, Inc., 2016

    2016-01-01

    "The Condition of College and Career Readiness" looks at the progress of the 2016 ACT®-tested graduating class relative to college and career readiness. This state briefing begins with statistics in the following categories for Montana: performance, STEM, career readiness, impact, behaviors that impact access and opportunity, pipeline,…

  1. Native American Literature: Montana and Northcentral Regional Publications.

    ERIC Educational Resources Information Center

    Susag, Dorothea

    This annotated bibliography is designed to help Montana K-12 teachers integrate authentic Native American literature into their curricula. A thematic approach to literature is encouraged, so that students may make meaningful connections between the classroom and their personal worlds. Accordingly, the entries are categorized according to the…

  2. A new fauna from the Colorado group of southern Montana

    USGS Publications Warehouse

    Reeside, John B.

    1925-01-01

    This paper describes a small but interesting fauna collected in 1921 by W. T. Thorn, Jr., Gail F. Moulton, T. W. Stanton, and K. C. Heald in the Crow Indian Reservation in southern Montana. The locality is in sec. 36, T. 6 S., R. 32 E., Big Horn County, and is 2 miles east of the Soap Creek oil field.

  3. 2011 Montana Youth Risk Behavior Survey: Nonpublic Accredited Schools

    ERIC Educational Resources Information Center

    Montana Office of Public Instruction, 2011

    2011-01-01

    This report presents the 2011 Montana Youth Risk Behavior Survey high school student frequency distributions for nonpublic accredited schools. These frequency distributions are based upon surveys with 349 high school students in Nonpublic Region during February of 2011. Frequency distributions may not total 349 due to nonresponse and percents may…

  4. Vascular plants of west-central Montana-identification guidebook

    Treesearch

    Klaus Lackschewitz

    1991-01-01

    This comprehensive guidebook provides keys, illustrations, and descriptions that aid identification of the 1,600+ species and varieties of vascular plants growing in west-central Montana. The area covered encompasses Ravalli County and southern Missoula County, and it includes the Bitterroot National Forest and portions of the Lolo National Forest. This guidebook uses...

  5. Montana Consolidated State Application Accountability Workbook. Further Amended and Resubmitted

    ERIC Educational Resources Information Center

    US Department of Education, 2006

    2006-01-01

    This workbook, submitted by the State of Montana to the U.S. Department of Education, is for State Grants under Title IX, Part C, Section 9302 of the Elementary and Secondary Education Act (Public Law 107-110). By January 31, 2003, States must complete and submit to the Department this Consolidated State Application Accountability Workbook. The…

  6. Hydrogen Futures Park at the University of Montana. Final report

    SciTech Connect

    Williamson, R Paul

    2010-11-18

    The Hydrogen Futures Park at the University of Montana, was conceived and presented as a premier, paradigm-shifting plan to foster the creation of a sustainable, platinum, LEED-certified college campus providing technical, safety and across the curriculum energy education and training.

  7. Hierarchical den selection of Canada lynx in western Montana

    Treesearch

    John R. Squires; Nicholas J. Decesare; Jay A. Kolbe; Leonard F. Ruggiero

    2008-01-01

    We studied den selection of Canada lynx (Lynx canadensis; hereafter lynx) at multiple ecological scales based on 57 dens from 19 females located in western Montana, USA, between 1999 and 2006. We considered 3 spatial scales in this analysis, including den site (11-m-radius circle surrounding dens), den area (100-m-radius circle), and den environ (1-...

  8. Winter prey selection of Canada lynx in northwestern Montana

    Treesearch

    John R. Squires; Leonard F. Ruggiero

    2007-01-01

    The roles that diet and prey abundance play in habitat selection of Canada lynx (Lynx canadensis) in the contiguous United States is poorly understood. From 1998-2002, we back-tracked radiocollared lynx (6 F, 9 M) for a distance of 582 km and we located 86 kills in northwestern Montana, USA. Lynx preyed on 7 species that included blue grouse (Dendragapus...

  9. Abundance and characteristics of snags in western Montana forests

    Treesearch

    Richard B. Harris

    1999-01-01

    Plot data from the U.S. Forest Service's Forest Inventory and Analysis program was used to characterize the abundance and selected characteristics of snags from forests in western Montana. Plots were grouped by whether they had a history of timber harvest, and the U.S. Forest Service classifications of forest type, habitat type, and potential vegetation group were...

  10. Grassland and shrubland habitat types of western Montana

    Treesearch

    W. F. Mueggler; W. L. Stewart

    1978-01-01

    A classification system based upon potential natural vegetation is presented for the grasslands and shrublands of the mountainous western third of Montana. The classification was developed by analyzing data from 580 stands. Twenty-nine habitat types in 13 climax series are defined and a diagnostic key provided for field identification. Environment, vegetative...

  11. Off-Farm Agricultural Occupations in Montana: Employment and Education.

    ERIC Educational Resources Information Center

    Heaney, Donald S.

    Data were obtained by interview with managers in a random sample of 161 businesses in 10 selected Montana trade and service centers to (1) identify present and emerging off-farm agricultural occupations, (2) estimate present and anticipated numbers of employees, (3) estimate annual entry opportunities, (4) determine needed competencies, and (5)…

  12. Attraction and Retention of Students at the University of Montana.

    ERIC Educational Resources Information Center

    Corak, Kitty

    Reasons that students enter or leave the University of Montana (UM) were studied in fall 1983. Consideration was given to first-term freshmen of traditional age, slightly older students (under age 25), transfer students, dropouts, and students who persisted at the university for 1 academic year. In study phase 1, attention was directed to…

  13. 76 FR 59479 - Montana Disaster Number MT-00062

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-26

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Montana Disaster Number MT-00062 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2... Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster...

  14. ACT Profile Report: State. Graduating Class 2016. Montana

    ERIC Educational Resources Information Center

    ACT, Inc., 2016

    2016-01-01

    This report provides information about the performance of Montana's 2016 graduating seniors who took the ACT as sophomores, juniors, or seniors; and self-reported at the time of testing that they were scheduled to graduate in 2016. Beginning with the Graduating Class of 2013, all students whose scores are college reportable, both standard and…

  15. The Funding Book: Rural Supported Employment Resources for Montana.

    ERIC Educational Resources Information Center

    Montana Univ. Affiliated Rural Inst., Missoula.

    This guide provides information on funding resources for supported employment in Montana. Supported employment provides individuals with severe disabilities the tools necessary to enter into an integrated work setting at a competitive wage. Supported employment generally consists of the professional expertise of a job coach, job development…

  16. Wilderness Institute School of Forestry, University of Montana.

    ERIC Educational Resources Information Center

    Wall, Kenneth

    1987-01-01

    Describes one of the various environmental studies programs offered at the University of Montana. Focuses on the school of forestry's Wilderness Institute which deals with wilderness preservation and management. Deals with the institute's responses to changes in wilderness issues, student interests, and information technology. (TW)

  17. Information-Related Competencies for Montana Extension Service Professionals.

    ERIC Educational Resources Information Center

    Kawasaki, Jodee Lynn

    Information-related competencies and training needed by professionals in the Montana Extension Service in order to acquire and disseminate information to clients electronically were studied in a needs assessment of administrators, specialists, and extension agents. An e-mail survey listing 37 competencies was completed by 96 extension agents and…

  18. Effectiveness of SOE in Montana's Vocational Agriculture Program.

    ERIC Educational Resources Information Center

    Van Shelhamer, C.

    A study was designed to determine the strengths and weaknesses of Supervised Occupational Experience (SOE) in Montana and the extent to which vocational agriculture teachers were using it to enhance their students' education. The student population consisted of 144 vocational agriculture students who had submitted records on SOE projects. All…

  19. Montana Partnerships for Rural Resource Teams Project Manual.

    ERIC Educational Resources Information Center

    Montana State Office of Public Instruction, Helena.

    This project manual from the Partnerships for Rural Resource Teams Project, a project designed to build upon the foundation of services for children and youth with deaf-blindness in Montana, opens with guiding principles of the project and a description of the types of available services. Grounded in a framework that draws upon the principles of…

  20. 76 FR 63323 - Notice of Competitive Coal Lease Sale, Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-12

    ...-L13200000-EL0000-P; MTM 97988] Notice of Competitive Coal Lease Sale, Montana AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: Notice is hereby given that the coal reserves in the lands... by application (LBA) filed by Signal Peak Energy LLC. The Federal coal resource to be...

  1. 77 FR 2316 - Notice of Competitive Coal Lease Sale, Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-17

    ... Bureau of Land Management Notice of Competitive Coal Lease Sale, Montana AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: Notice is hereby given that the coal reserves in the lands... lease by application (LBA) filed by Signal Peak Energy LLC. The Federal coal resource to be...

  2. 75 FR 15411 - Southwest Montana Resource Advisory Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... Monday April 19, 2010, from 1 p.m. until 4 p.m., in Dillon, Montana. The purpose of the meeting is to review funding proposals for Title II funding. DATES: Monday, April 19, 2010, from 1 p.m. until 4...

  3. Montana Indian Law-Related Education Model Curriculum Guide.

    ERIC Educational Resources Information Center

    Monprode-Holt, Lorrie; And Others

    This curriculum guide, it is hoped, will become an integral part of the social studies curriculum for all Montana students. Focusing on law-related education themes and concepts, six broad-based themes are covered: responsibility, authority, privacy, justice, spirituality, and environment. These six themes are found in the sample lessons under the…

  4. National Environmental/Energy Workforce Assessment for Montana.

    ERIC Educational Resources Information Center

    National Field Research Center Inc., Iowa City, IA.

    This report presents existing workforce levels, training programs and career potentials and develops staffing level projections (1976-1982) based on available information for the State of Montana. The study concerns itself with the environmental pollution control areas of air, noise, potable water, pesticides, radiation, solid waste, wastewater,…

  5. 78 FR 3026 - Establishment of Swan Valley Conservation Area, Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-15

    ... Fish and Wildlife Service Establishment of Swan Valley Conservation Area, Montana AGENCY: Fish and... Wildlife Service (Service) has established the Swan Valley Conservation Area as a unit of the National Wildlife Refuge System. The Service established the Swan Valley Conservation Area on August 6, 2012,...

  6. Handbook for Faculty and Staff of Eastern Montana College.

    ERIC Educational Resources Information Center

    Eastern Montana Coll., Billings.

    Eastern Montana College is a comprehensive state college. The faculty handbook discusses: (1) organization and administration of the college; (2) responsibilities of administrative officers and faculty advisors; (3) committee composition and function; (4) faculty organization and regulations; (5) academic regulations; (6) campus policies; and (7)…

  7. On-site energy consumption at softwood sawmills in Montana

    Treesearch

    Dan Loeffler; Nathaniel Anderson; Todd A. Morgan; Colin B. Sorenson

    2016-01-01

    Total on-site energy requirements for wood product manufacturing are generally not well understood or publicly available, particularly at subregional scales, such as the state level. This article uses a mail survey of softwood sawmills in Montana to develop a profile of all on-site energy consumption. Energy use is delineated by fuel type on a production basis...

  8. Plant water status relationships among major floodplain sites of the Flathead River, Montana

    USGS Publications Warehouse

    Lee, L.C.; Hinckley, T.M.; Scott, M.L.

    1985-01-01

    Water status measurements of dominant species from major floodplain plant community types of the North Fork Flathead River, Montana were used to test the accuracy of site moisture gradient relationships postulated from floristic ordinations and site water balance estimates. Analysis of variance tests showed significant differences among the average predawn xylem pressure potential (ψp) of species in several community types. However, additional analyses failed to indicate a significant degree of association between averaged predawn Yp measurements and either floristic ordination or site water balance results. Sixty eight percent of 22 trials comparing the diurnal average ψp of the same species in different community types on the same day were less negative for a species in the wetter community types as predicted by floristic ordinations. Similarly, 64% of the trials indicated that the diurnal average stomatal conductance was higher for a species in the wetter type. These results suggest that although a floodplain moisture gradient exists, it alone does not limit the distribution of floodplain plant communities in the North Fork.

  9. Supplementation with soybean peptides, taurine, Pueraria isoflavone, and ginseng saponin complex improves endurance exercise capacity in humans.

    PubMed

    Yeh, Tzu-Shao; Chan, Kuei-Hui; Hsu, Mei-Chich; Liu, Jen-Fang

    2011-03-01

    The purpose of this study was to investigate the effects of a proprietary blend of soybean peptides, taurine, Pueraria isoflavone, and ginseng saponin complex (STPG capsule) on exercise performance in humans. Fourteen male volunteers were randomly assigned to two crossover treatments in which they consumed either four STPG capsules (STPG treatment) or placebo (P treatment) for 15 days before a 75% maximal oxygen uptake (VO(2max)) exhaustive cycling test. Blood samples and respiratory gas were collected prior to the exercise (Pre-Ex), at 10 (Ex-10), 15 (Ex-15), 20 (Ex-20), and 25 (Ex-25) minutes during exercise, and immediately after exercise (exhaustion) to assess the blood metabolites, cardiorespiratory responses, and energy substrate utilization. The result showed that exercise time to exhaustion of the 75% (VO(2max)) exhaustive cycling test of the STPG-treated subjects was significantly greater than with the P treatment (30.99 ± 2.01 vs. 28.05 ± 1.48 minutes). The plasma lactate concentrations at Ex-20 and Ex-25 in the STPG treatment were significantly lower with STPG treatment than with P treatment (10.5 ± 0.7 vs. 11.5 ± 0.8 and 10.7 ± 0.9 vs.12.3 ± 1.0 mmol/L, respectively). Nonesterified fatty acid levels at Ex-15, Ex-20, Ex-25, and exhaustion in the STPG group (0.27 ± 0.03, 0.32 ± 0.04, 0.32 ± 0.06, and 0.37 ± 0.05 mmol/L, respectively) were significantly higher than those in the P treatment (0.21 ± 0.03, 0.23 ± 0.03, 0.24 ± 0.03, and 0.25 ± 0.03 mmol/L, respectively). It was concluded that supplementation of four capsules (2 g) of STPG complex, consisting of soybean peptides, taurine, Pueraria isoflavone, and ginseng saponin, for 15 days was effective in promoting utilization of free fatty acids and improving exhaustive cycling test performance in humans.

  10. Establishment, succession, and stability of vegetation on surface mined lands in eastern Montana. Annual progress report

    SciTech Connect

    Sindelar, B. W.; Plantenberg, P. L.

    1980-07-01

    An ecological investigation of reclamation and plant succession on surface mined land near Colstrip, Montana, was initiated in June 1975. Its purpose was to examine and document development, stability, and permanence of plant communities established on semiarid mined land since 1928. This report includes analyses of data collected through four growing seasons on study sites located on naturally revegetated spoil and on seeded spoil. Sites included revegetated spoil seeded in 1969, 1970, 1971, 1972, 1973, 1975, and 1977. Naturally revegetated spoil was mined in 1928 and 1930. Data collected include plant density, frequency, cover, yield, composition, species diversity, and soil moisture content. Previous progress reports have detailed successional changes and patterns observed. Analyses of causal factors are included. This report summarizes successional changes which occurred during the past year, presents updated analyses of successional trends and patterns, and presents additional conclusions derived from these analyses.

  11. Space Radar Image of Rocky Mountains, Montana

    NASA Technical Reports Server (NTRS)

    1994-01-01

    This is a three-dimensional perspective of the eastern front range of the Rocky Mountains, about 120 kilometers (75 miles) west of Great Falls, Montana. The image was created by combining two spaceborne radar images using a technique known as interferometry. Visualizations like this are useful to scientists because they show the shapes of the topographic features such as mountains and valleys. This technique helps to clarify the relationships of the different types of materials on the surface detected by the radar. The view is looking south-southeast. Along the right edge of the image is the valley of the north fork of the Sun River. The western edge of the Great Plains appears on the left side. The valleys in the lower center, running off into the plains on the left, are branches of the Teton River. The highest mountains are at elevations of 2,860 meters (9,390 feet), and the plains are about 1,400 meters (4,500 feet) above sea level. The dark brown areas are grasslands, bright green areas are farms, light brown, orange and purple areas are scrub and forest, and bright white and blue areas are steep rocky slopes. The two radar images were taken on successive days by the Spaceborne Imaging Radar-C/X-band Synthetic Aperture Radar (SIR-C/X-SAR) on board the space shuttle Endeavour in October 1994. The digital elevation map was produced using radar interferometry, a process in which radar data are acquired on different passes of the space shuttle. The two data passes are compared to obtain elevation information. Radar image data are draped over the topography to provide the color with the following assignments: red is L-band vertically transmitted, vertically received; green is C-band vertically transmitted, vertically received; and blue are the differences seen in the L-band data between the two days. This image is centered near 47.7 degrees north latitude and 112.7 degrees west longitude. No vertical exaggeration factor has been applied to the data. SIR-C/X-SAR, a

  12. Mountain plover responses to plague in Montana.

    PubMed

    Dinsmore, Stephen J; Smith, Mark D

    2010-01-01

    Plague is a bacterial (Yersinia pestis) disease that causes epizootic die-offs in black-tailed prairie dog (Cynomys ludovicianus) populations in the North American Great Plains. Through their grazing and burrowing, prairie dogs modify vegetation and landscape structure on their colonies in ways that affect other grassland species. Plague epizootics on prairie dog colonies can have indirect effects on species associated with colonies. The mountain plover (Charadrius montanus) preferentially nests on black-tailed prairie dog colonies and is thus negatively impacted by the loss of prairie dogs. We studied the effects of plague and colony spatial characteristics on the occupancy of 81 prairie dog colonies by nesting plovers in Phillips County, Montana, during a 13-year period (1995-2007). We used a robust design patch occupancy model to investigate how colony occupancy and extinction and colonization rates were affected by plague history, colony size, and colony shape. Here extinction and colonization rates refer to the probability that a colony loses/gains plovers in a subsequent nesting season, given that it had/lacked plovers in that breeding season. Colony occupancy was best explained by a model with no annual variation or plague effects. Colony extinction rates were driven by a combination of a quadratic of colony area, a 3-year plague response, and a measure of colony shape. Conversely, colonization rates were best explained by a model with a 4-year plague response. The estimated annual proportion of colonies occupied by plovers was 0.75 (95% confidence interval = 0.57-0.87). Estimated extinction probability ranged from a low of 0.07 (standard error [SE] = 0.02) in 2002 to a high of 0.25 (SE = 0.03) in 1995; colonization probability ranged from 0.24 (SE = 0.05) in 2006 to 0.35 (SE = 0.05) in 2000. Our results highlight how a bird that depends on prairie dogs for nesting habitat responds to plague history and other spatial characteristics of the colony. Ultimately

  13. Smokers Report: A Health Risk Behavior Comparison of Montana High School Students Based on Current Smoking. 2011 Montana Youth Risk Behavior Survey

    ERIC Educational Resources Information Center

    Montana Office of Public Instruction, 2013

    2013-01-01

    The Montana Youth Risk Behavior Survey (YRBS) is administered by the Montana Office of Public Instruction every two years to students in grades 7 through 12. The purpose of the survey is to help monitor the prevalence of behaviors that not only influence youth health, but also put youth at risk for the most significant health and social problems…

  14. Students with Special Needs: A Health Risk Behavior Comparison of Montana High School Students Based on Special Education Assistance. 2011 Montana Youth Risk Behavior Survey

    ERIC Educational Resources Information Center

    Montana Office of Public Instruction, 2012

    2012-01-01

    The Montana Youth Risk Behavior Survey (YRBS) is administered by the Montana Office of Public Instruction every two years to students in grades 7 through 12. The purpose of the survey is to help monitor the prevalence of behaviors that not only influence youth health, but also put youth at risk for the most significant health and social problems…

  15. Sports Team Participation: A Risk Behavior Comparison of Montana High School Students Based on Sports Team Participation. 2011 Montana Youth Risk Behavior Survey

    ERIC Educational Resources Information Center

    Montana Office of Public Instruction, 2012

    2012-01-01

    The Montana Youth Risk Behavior Survey (YRBS) is administered by the Montana Office of Public Instruction every two years to students in grades 7 through 12. The purpose of the survey is to help monitor the prevalence of behaviors that not only influence youth health, but also put youth at risk for the most significant health and social problems…

  16. Health-hazard evaluation report HETA-84-043, HETA 84-425-1680, Montana log home residents, Kalispell, Montana. [Treated with pentachlorophenol preservatives

    SciTech Connect

    Lee, S.A.

    1986-04-01

    Environmental and urine sampling was conducted in 15 log homes in Montana previously treated with wood preservatives containing pentachlorophenol (PCP). The Montana Department of Health had requested assistance in evaluating exposures to PCP among state residents in PCP-treated log homes.

  17. Determination of the estrogenic activity of wild phytoestrogen-rich Pueraria mirifica by MCF-7 proliferation assay.

    PubMed

    Cherdshewasart, Wichai; Traisup, Virasinee; Picha, Porntipa

    2008-02-01

    The aim of this study was to evaluate the estrogenic activity of tuberous samples of wild, phytoestrogen-rich Pueraria mirifica collected from 28 out of 76 provinces of Thailand by MCF-7 proliferation assay. The plant extracts were administered to MCF-7, ER alpha positive human mammary adenocarcinoma cell cultures, for 3 days at dosages of 0.1, 1, 10, 100 and 1,000 microg/ml and were compared with 17 beta-estradiol at concentrations of 10(-12)-10(-6) M. The mean P. mirifica population at 1 mug/ml exhibited significant proliferation. Two plant samples exhibited levels of proliferation in MCF-7 that were similar to 17beta-estradiol. The mean P. mirifica populations at 100 and 1,000 microg/ml exhibited significant cytotoxicity in MCF-7. Analysis of the estrogenic activity of puerarin, representative of major isoflavonoids in P. mirifica tubers, revealed proliferation in MCF-7 only at the highest dose (10(-6) M) that was 10(2)-10(5) times less active than 17 beta-estradiol. Puerarin and 17 beta-estradiol at concentration of 10(-12)-10(-6) M exhibited no cytotoxicity in MCF-7.

  18. Molecular Cloning and Functional Characterization of a Novel Isoflavone 3′-O-methyltransferase from Pueraria lobata

    PubMed Central

    Li, Jia; Li, Changfu; Gou, Junbo; Zhang, Yansheng

    2016-01-01

    Pueraria lobata roots accumulate 3′-, 4′- and 7-O-methylated isoflavones and many of these methylated compounds exhibit various pharmacological activities. Either the 4′- or 7-O-methylation activity has been investigated at molecular levels in several legume species. However, the gene encoding the isoflavone 3′-O-methyltransferase (OMT) has not yet been isolated from any plant species. In this study, we reported the first cDNA encoding the isoflavone 3′-OMT from P. lobata (designated PlOMT4). Heterologous expressions in yeast and Escherichia coli cells showed that the gene product exhibits an enzyme activity to methylate the 3′-hydroxy group of the isoflavone substrate. The transcript abundance of PlOMT4 matches well with its enzymatic product in different organs of P. lobata and in the plant roots in response to methyl jasmonate elicitation. Integration of the biochemical with metabolic and transcript data supported the proposed function of PlOMT4. The identification of PlOMT4 would not only help to understand the isoflavonoid metabolism in P. lobata but also potentially provide an enzyme catalyst for methylating existing drug candidates to improve their hydrophobicity. PMID:27458460

  19. Supplementation of Pueraria radix water extract on changes of antioxidant enzymes and lipid profile in ethanol-treated rats.

    PubMed

    Lee, Jeong-Sook

    2004-09-01

    Water extract of Pueraria radix (PRWE), traditional oriental medicinal plant, may have an effect on the activity of hepatic antioxidant enzymes and lipid profile in ethanol-treated rats. Male Sprague-Dawley rats were divided into control, ethanol, PRWE and ethanol-PRWE supplemented groups. Twenty-five percent (v/v) ethanol (5 g/kg body weight) was orally administered once a day for 5 weeks. The PRWE was supplemented in a diet based on 1500 mg of raw PRWE/kg body weight/day. Ethanol feeding resulted in a higher alcohol dehydrogenase (ADH) activity and lower aldehyde dehydrogenase (ALDH) activity. After PRWE supplementation, both activities were increased. The PRWE supplementation resulted in a significant decrease in the plasma and liver total cholesterol concentrations in the ethanol-treated rats. Ethanol administration significantly lowered the activities of hepatic superoxide dismutase (SOD) and catalase (CAT), whereas it increased the plasma and hepatic thiobarbituric acid reactive substances (TBARS) and the hepatic glutathione peroxidase (GSH-Px) activities. However, PRWE supplementation resulted in a significant increase in the SOD and CAT activities and a significant decrease in the TBARS and the GSH-Px activities in the ethanol-treated rats. PRWE can contribute to alleviating the adverse effect of ethanol ingestion by enhancing the lipid metabolism as well as the hepatic antioxidant defense system.

  20. Rapid simultaneous determination of major isoflavones of Pueraria lobata and discriminative analysis of its geographical origins by principal component analysis.

    PubMed

    Zhao, Chenxi; Chan, Hoi-yan; Yuan, Dalin; Liang, Yizeng; Lau, Tsui-yan; Chau, Foo-Tim

    2011-01-01

    Isoflavones are main bioactive components of Pueraria lobata (Willd.) Ohwi. Puerarin has been used as the marker compound in herb quality evaluation in the Chinese Pharmacopoiea. However, it is also important to include the other isoflavones present in the herb, such as daidzin, daidzein, genistin and genistein, in the evaluation as they also contribute to the overall bioactivity of the herb. To develop a rapid and reliable method for simultaneous quantitation of isoflavones for P. lobata herb quality evluation. The chromatographic separation was performed on an Agilent rapid resolution liquid chromatographic system through gradient elution. The developed method for the quantification of puerarin, daidzin, daidzein, genistin and genistein was fully validated. When it was applied to analyse the extracts of P. lobata, baseline separation was obtained within 10 min. The amounts of puerarin, daidzin, daidzein and genistin varied greatly among the samples although their chromatographic fingerprints were similar to each other. The 19 samples studied were classified into three clusters (I-III) by principal component analysis based on the amounts of puerarin, daidzin, daidzein and genistin. The classification result can be related to herbal origins, but the classification outcome from the chromatographic fingerprinting similarity approach did not provide any geographical origin information. This shows that bioactive constituents can reflect the intrinsic quality of P. lobata more accurately. Copyright © 2011 John Wiley & Sons, Ltd.

  1. [Simultaneous determination of tectorigenin-7-O-xylosylglucosid, tectoridin and tectorigenin in flowers of Pueraria lobata by HPLC].

    PubMed

    Liu, Lihui; Li, Weimin; Gao, Ying

    2010-09-01

    To develop a HPLC method for the simultaneous determination of tectorigenin-7-O-xylosylglucosid, tectoridin and tectorigenin in flowers of Pueraria lobata. The separation was performed on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with acetonitrile-water containing 0.2% phosphoric acid as the mobile phase. The flow rate was 1.0 mL x min(-1), the detection wavelength was 265 nm and the temperature of column was 35 degrees C. The linear ranges of tectorigenin-7-O-xylosylglucosid, tectoridin and tectorigenin were 0.14-1.40 (r = 0.9994), 0.20-2.02 (r = 0.9993), and 0. 10-1.04 (r = 0.9991) , respectively. The average recoveries (n=6) of the three constituents were 99.5% (RSD 1.8%), 99.6% (RSD 1.7%), and 99.8% (RSD 1.9%), respectively. The method is simple and accurate with a good eata and can be used as a quality control method for flowers of P. lobata of different sources.

  2. Anti-inflammatory effect of Pueraria tuberosa extracts through improvement in activity of red blood cell anti-oxidant enzymes

    PubMed Central

    Pandey, Nidhi; Yadav, Durgavati; Pandey, Vivek; Tripathi, Yamini B.

    2013-01-01

    Changing life style and over-nutrition causes low-grade inflammation (LGI), with obesity and hyper-lipidemia as basic factors. The physiological state polarizes macrophages to classical type (M1), which is pro-inflammatory and promotes ectopic fat deposition in the body. Both factors induce inflammatory cascade, where free radicals (FRs) play an important role. Thus, pharmacological and non-pharmacological interventions would be effective in the management of LGI and plant products would be used as food supplement or as a drug. Previously, a study has reported the anti-oxidant potential of methanolic extract of tubers of Pueraria tuberosa (PTME) and inhibitory role of tuberosin on lipopolysaccharides-induced expression of inducible nitric oxide synthase in macrophages in an in vitro study model. Here, the effect of PTME has been explored on carrageenan-induced inflammatory changes in rats. The activity of antioxidant enzymes in red blood cell hemolysate has been assessed. PTME was orally given to rats for 9 days and periodical changes (every 3rd day) in the activity/concentration of superoxide dismutase (SOD), catalase, reduced glutathione (GSH), lipid peroxides (LPO), and C-reactive proteins (CRP) were monitored. The PTME significantly prevented carrageenan-induced decline in GSH content, lowering of catalase and SOD activity, and rise in LPO and CRP in rats in a time-dependent, sequential manner. Thus, it could be suggested that the anti-inflammatory role of PTME is primarily mediated through its FR scavenging potential. PMID:24501527

  3. Beta-glucuronidase inhibitor tectorigenin isolated from the flower of Pueraria thunbergiana protects carbon tetrachloride-induced liver injury.

    PubMed

    Lee, Hae-Woong; Choo, Min-Kyung; Bae, Eun-Ah; Kim, Dong-Hyun

    2003-08-01

    To understand the relationship between the fluctuation in serum beta-glucuronidase and hepatotoxicity, an inhibitor of beta-glucuronidase was isolated from the flowers of Pueraria thunbergiana and its hepatoprotective activity was measured. Tectorigenin was isolated from the flowers of pueria thunbergiana as an inhibitor of beta-glucuronidase, and serum ALT, AST and biological parameters as markers for its hepatoprotective activity were measured on CCl4-induced liver injury in mice. The relationship between serum beta-glucuronidase and hepatoprotective activities in mice was measured. : When tectorigenin at a dose of 100 mg/kg was intraperitoneally administered on CCl4-induced liver injury in mice, it significantly inhibited the increase of plasma ALT, AST and LDH activities. The inhibitory effect of tectorigenin is much more potent than that of dimethyl diphenyl bicarboxylate (DDB), which has been used as a commercial hepatoprotective agent. When tectoridin transformed to tectorigenin by intestinal bacteria was orally administered to mice, it showed hepatoprotective activity. However, when tectoridin was intraperitoneally administrated to mice, it did not exhibit hepatoprotective activity. Moreover, orally administered tectoridin not only inhibited beta-glucuronidase but also increased GSH content and GST activity on CCl4-induced hepatotoxicity of mice. We insist that an inhibitor of beta-glucuronidase tectorigenin may be hepatoprotective and tectoridin should be a prodrug transformed to tectorigenin.

  4. Involvement of abscisic acid in ozone-induced puerarin production of Pueraria thomsnii Benth. suspension cell cultures.

    PubMed

    Sun, Lina; Su, Hu; Zhu, Yun; Xu, Maojun

    2012-01-01

    Exposure to ozone induced a rapid increase in the levels of the sesquiterpene phytohormone abscisic acid (ABA) and the isoflavone puerarin in suspension cell cultures of Pueraria thomsnii Benth. The observed increases in ABA and puerarin were dependent on the concentration of ozone applied to P. thomsnii cell cultures. In order to examine the role of ABA in ozone-induced puerarin production, cell suspensions were pretreated with the ABA biosynthetic inhibitor fluridone. Following ozone exposure, fluridone treatment suppressed ABA accumulation suggesting ABA was normally synthesized de novo through the carotenoid pathway. Fluridone also blocked ozone-induced puerarin production, which could be reversed through application of exogenous ABA. However, in the absence of ozone, ABA itself had no effect on puerarin accumulation in the suspension cells. Taken together, the data indicate that ozone is an efficient elicitor of puerarin production and may be particularly applicable for improving puerarin production in plant cell cultures. Furthermore, we demonstrate that ABA is one factor associated with ozone-induced puerarin production in P. thomsnii cell cultures.

  5. Effect of Pueraria tuberosa DC. (Indian Kudzu) on blood pressure, fibrinolysis and oxidative stress in patients with stage 1 hypertension.

    PubMed

    Verma, S K; Jain, Vartika; Singh, D P

    2012-08-01

    The Indian Kudzu (Pueraria tuberosa DC.) is an important medicinal plant widely used in Indian and Chinese traditional systems of medicine. The present study is an attempt to evaluate effect of its tubers on blood pressure, coagulation parameters and antioxidant status in patients with stage 1 (primary) hypertension. In a long-term, single blinded, placebo controlled study; 15 patients with stage 1 hypertension (group 1), were administered 3 g P. tuberosa in two divided doses while another 15 patients (group II) were administered matched placebo for a period of twelve weeks. A significant fall of 25, 11 and 16 mmHg was observed in systolic (p < 0.001), diastolic (p < 0.05) and mean (p < 0.001) blood pressure, respectively at the end of the study. Along with blood pressure reduction, there was a significant (p < 0.01) reduction in plasma fibrinogen and significant enhancement of plasma fibrinolytic activity (p < 0.001) and serum total antioxidant status (p < 0.05). It was tolerated well without any untoward side effects.

  6. Molecular cloning and functional characterization of two divergent 4-coumarate : coenzyme A ligases from Kudzu (Pueraria lobata).

    PubMed

    Li, Zhao-Bo; Li, Chang-Fu; Li, Jia; Zhang, Yan-Sheng

    2014-01-01

    As part of the efforts to understand isoflavonoid metabolism in Pueraria lobata at the molecular level, the cDNAs encoding two divergent 4-coumarate : coenzyme A ligases (4CLs, designated Pl4CL1 and Pl4CL2, respectively) were isolated from P. lobata roots. Sequence analysis revealed that Pl4CL1 had an N-terminal extension of twenty-one amino acid residues compared to Pl4CL2. Phylogenetic analysis showed that Pl4CL1 and Pl4CL2 fell into angiosperm Class II and Class I, respectively. Through in vitro biochemical assays, both Pl4CLs were found to have the capacity to utilize 4-coumarate and trans-cinnamate as substrates, while neither of them could convert sinapate. Pl4CL2 had a broader substrate specificity than Pl4CL1. The affinity of Pl4CL1 for 4-coumarate was 2.6-fold higher than that of Pl4CL2 (with the Km values of 3.5 µM and 9.1 µM, respectively). Combining the dataset including gene expression profiles, metabolites measurements, and biochemical properties, our results indicated that Pl4CL1, just as other angiosperm Class II 4CLs, might play a role in isoflavone biosynthesis in P. lobata, while Pl4CL2 belongs to angiosperm Class I, and may function as a housekeeping enzyme concerning lignification.

  7. Protection of HT22 neuronal cells against glutamate toxicity mediated by the antioxidant activity of Pueraria candollei var. mirifica extracts.

    PubMed

    Sucontphunt, Apirada; De-Eknamkul, Wanchai; Nimmannit, Ubonthip; Dan Dimitrijevich, S; Gracy, Robert W

    2011-01-01

    Neuronal degeneration is known to be due to oxidative stress acting through a pathway involving the excessive activation of glutamate receptors. We studied the neuroprotection potential of an ethyl acetate-ethanol extract of Pueraria mirifica (P. candollei var. mirifica) root (PM extract). PM extract was evaluated for its antioxidant and neuroprotective activities against glutamate toxicity in mouse hippocampal HT22 neuronal cells. The extract at concentrations of 10 and 50 μg/ml exhibited considerable antioxidant activity with significant neuroprotection, based on the microscopic observations of cell morphology and the determination of cell viability and cell number. Studies of the possible mechanisms of action indicated that the neuroprotection exerted by PM extract was related to its scavenging activity against H(2)O(2) and related reactive oxygen species. High-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) analyses showed that the extract contained daidzein and genistein as identified constituents, as well as additional components with antioxidant activity. While daidzein and genistein individually and in combination were observed not to be neuroprotective, we propose that the antioxidant and neuroprotective activities of PM extract are derived from the combined properties of its constituents.

  8. [Effects of 6-benzylaminopurine and α-naphthaleneacetic acid on growth and isoflavone contents of Pueraria phaseoloides hairy roots].

    PubMed

    He, Hanjie; Shi, Heping

    2014-10-01

    In order to study the effect of phytohormone on growth and isoflavones contents of Pueraria phaseoloides hairy roots, we cultured the hairy roots with different concentrations of 6-benzylaminopurine (6-BA) alone or in combination with α-naphthaleneacetic acid (NAA). Then we determined the effects of 6-BA alone or in combination with NAA on the growth and the contents of isoflavones compounds and levels of antioxidase activities of hairy roots by spectrophotometry. The results show that 6-BA inhibited the growth, and decreased biomass and total isoflavones compounds of P. phaseoloides hairy roots. Furthermore, the inhibition was increased with the concentrations of 6-BA. Compared with the controls, different concentrations of 6-BA in combination with NAA 2.0 mg/L could inhibit the growth of hairy roots and decrease the content of total isoflavone compounds, and also significantly enhanced the contents of soluble protein and levels of peroxidase (POD) activities, but decreased the activities of superoxide dismutase (SOD). DNA ladders detected by agarose gel electrophoresis can be observed after hairy roots of P. phaseoloides were cultured with 6-BA alone for 30 days, but can appear on the 20th day after culture with 6-BA in combination with NAA 2.0 mg/L. This result indicates that 6-BA or 6-BA in combination with NAA can both stimulate appearance of programmed cell death (PCD), and NAA may play a synergistic role on PCD.

  9. Cultural Resource Survey, Government Townsites Study, Fort Peck, Montana, Pickstown, South Dakota, Riverdale, North Dakota,

    DTIC Science & Technology

    1980-05-01

    Fort Peck, Montana ZA ’ . Plate 6: Recreation Building Fort Peck, Montana 20 Plate 7: Lutheran Church Fort Peck, Montana Poo Plate 8: Lutheran Church...Joel Barker, Chief of Archives, Federal Archives and Records Center, Denver, Colorado. July 27, August 13, 1979. _ Interview with Dolores Barnard...Records Center, Denver, Colorado Dolores Barnard, Librarian, State Historical Society of North Dakota Library, Bismarck, North Dakota Leonard Bingham

  10. Baxter v. Montana, libertarianism, and end-of-life: the ripe time for a paradigm shift.

    PubMed

    Ruble, James H

    2010-09-01

    Baxter v. Montana (2009 WL 5155363 [Mont. 2009]) is a recent decision from the Montana Supreme Court that provides new legal insight into the societal issue of aid in dying. This case involves interests of persons with terminal illness, medical practitioners, law enforcement, legislative and judicial bodies, as well as the citizens of Montana. A summary judgment ruling at the Montana district court level was based almost entirely on a constitutional fundamental rights analysis. In contrast, the Montana Supreme Court affirming decision was based almost entirely on a statutory rights analysis. Both rulings from the Montana courts support the position that licensed prescribers in Montana who provide aid in dying assistance to terminally ill patients have some immunity from criminal prosecution. Each side in the case argued what they believed to be the intents and purposes of the people of Montana. Baxter v. Montana illustrates different methods to determine the will of the people concerning aid in dying and public policy. This case very subtly suggests a paradigm shift may be occurring in aid in dying policy.

  11. Ecological assessment of streams in the Powder River Structural Basin, Wyoming and Montana, 2005-06

    USGS Publications Warehouse

    Peterson, D.A.; Wright, P.R.; Edwards, G.P.; Hargett, E.G.; Feldman, D.L.; Zumberge, J.R.; Dey, Paul

    2009-01-01

    Energy and mineral development, particularly coalbed natural gas development, is proceeding at a rapid pace in the Powder River Structural Basin (PRB) in northeastern Wyoming. Concerns about the potential effects of development led to formation of an interagency working group of primarily Federal and State agencies to address these issues in the PRB in Wyoming and in Montana where similar types of resources exist but are largely undeveloped. Under the direction of the interagency working group, an ecological assessment of streams in the PRB was initiated to determine the current status (2005–06) and to establish a baseline for future monitoring.The ecological assessment components include assessment of stream habitat and riparian zones as well as assessments of macroinvertebrate, algal, and fish communities. All of the components were sampled at 47 sites in the PRB during 2005. A reduced set of components, consisting primarily of macroinvertebrate and fish community assessments, was sampled in 2006. Related ecological data, such as habitat and fish community data collected from selected sites in 2004, also are included in this report.The stream habitat assessment included measurement of channel features, substrate size and embeddedness, riparian vegetation, and reachwide characteristics. The width-to-depth ratio (bankfull width/bankfull depth) tended to be higher at sites on the main-stem Powder River than at sites on the main-stem Tongue River and at sites on tributary streams. The streambed substrate particle size was largest at sites on the main-stem Tongue River and smallest at sites on small tributary streams such as Squirrel Creek and Otter Creek. Total vegetative cover at the ground level, understory, and canopy layers ranged from less than 40 percent at a few sites to more than 90 percent at many of the sites. A bank-stability index indicated that sites in the Tongue River drainage were less at risk of bank failure than sites on the main-stem Powder River

  12. Campfire Stories of the Fort Belknap Community.

    ERIC Educational Resources Information Center

    Allen, Minerva, Comp.

    This collection contains 21 brief stories told by members of the Fort Belknap (Montana) American Indian community. These tales of the Assiniboine, Sioux, and Gros Ventres include legends, ghost stories, and reminiscences of heroic deeds, traditional life, and unusual events. Recollections of the past contain descriptions of the daily life of the…

  13. Campfire Stories of the Fort Belknap Community.

    ERIC Educational Resources Information Center

    Allen, Minerva, Comp.

    This collection contains 21 brief stories told by members of the Fort Belknap (Montana) American Indian community. These tales of the Assiniboine, Sioux, and Gros Ventres include legends, ghost stories, and reminiscences of heroic deeds, traditional life, and unusual events. Recollections of the past contain descriptions of the daily life of the…

  14. Environmental assessment of an aircraft conversion, Montana Air National Guard, Great Falls, Montana

    SciTech Connect

    Williams, G.; Policastro, A.; Krummel, J.; Pearl, R.; Trevorrow, L.; Hoffecker, J.

    1986-08-01

    It is proposed that the 120th Fighter Interceptor Group of the Montana Air National Guard convert from 18 F-106 to 18 F-16 aircraft. Associated with this conversion are building modifications, land acquisition, and facility construction. The environmental assessment determined that the primary impacts of the conversion would be positive. Noise modeling using the NOISEMAP methodology showed that the maximum noise reduction, resulting from the conversion, at any ground receptor point is about 5 dB on the L/sub dn/ scale. The noise reductions vary with the distance of a receptor point from the runways - the greater the distance, the smaller the noise reduction. Conversion to the F-16 prior to completion of a ''hush house'' would result in a temporary increase in noise to the southeast of the airport over a commercial and industrial area. In addition, total air pollutant emissions from aircraft operations would be reduced as a consequence of the conversion. No significant adverse impacts are predicted as a result of the conversion from F-106s to F-16s.

  15. Montana Rivers Information System : Edit/Entry Program User's Manual.

    SciTech Connect

    United States. Bonneville Power Administration; Montana Department of Fish, Wildlife and Parks

    1992-07-01

    The Montana Rivers Information System (MRIS) was initiated to assess the state`s fish, wildlife, and recreation value; and natural cultural, and geologic features. The MRIS is now a set of data bases containing part of the information in the Natural Heritage Program natural features and threatened and endangered species data bases and comprises of the Montana Interagency Stream Fisheries Database; the MDFWP Recreation Database; and the MDFWP Wildlife Geographic Information System. The purpose of this User`s Manual is to describe to the user how to maintain the MRIS database of their choice by updating, changing, deleting, and adding records using the edit/entry programs; and to provide to the user all information and instructions necessary to complete data entry into the MRIS databases.

  16. Floods of January 15-17, 1974, in Northwestern Montana

    USGS Publications Warehouse

    Johnson, M.V.; Omang, R.J.

    1974-01-01

    Introduction Rain and snowmelt caused several streams in northwestern Montana to flood during January 15-17, 1974. The flooding was caused by a major rainstorm that extended from Montana through Idaho and Washington. The most damage occurred in the Kootenai River drainage, centering around the town of Libby. Also unusually high flows occurred on some streams in the Clark Fork basin. Throughout the area, ice jams contributed to much of the overbank flooding. Both the towns of Troy and Libby were partly flooded, but damage was greatest in and near Libby. Flood damage was estimated to be $4.9 million. No flood fatalities were listed. Most of the smaller streams equaled or exceeded a 50-year flood. A summary of flood stages and discharges for 24 sites in the Kootenai River drainage and 8 sites in the Clark Fork drainage is tabulated. A map shows the extent of flooding in the Libby area. (Woodard-USGS)

  17. Montana Organization for Research in Energy (MORE) Final Report

    SciTech Connect

    Bromenshenk, Jerry

    1999-12-31

    MORE is a consortium of educational, governmental, and industrial partners in cooperation with the state's Tribal colleges. Formed in 1994, the objectives are to develop and promote energy-related research and education in the state of Montana and the Northwestern region. Specifically, they set out to: (1) promote collaboration and cooperation among Montana's Colleges and Universities; (2) maximize use of existing personnel and resources; (3) foster partnerships with industries, state agencies, and tribal nations; and (4) enhance energy research and training. The 1st Implementation Grant consisted of Management and Coordination, Human Outreach, and two Research Clusters Petroleum Reservoir Characterization and Wind Energy. Overall, they consider this program to have been highly successful. That conclusion was mirrored by the DOE site reviewers, and by invitations from Dr. Matesh Varma, the DOE/EPSCoR National Program Director, to present their programs and outcomes as models for other states the National DOE/EPSCoR meetings.

  18. Uses, funding, and availability of continuous streamflow data in Montana

    USGS Publications Warehouse

    Shields, R.R.; White, M.K.

    1984-01-01

    This report documents the results of a study of the uses, funding, and availability of continuous streamflow data collected and published by the U.S. Geological Survey in Montana. Data uses and funding sources are identified for the 218 continuous streamflow gages currently (1984) being operated. These stations are supported by 18 different funding sources at a budget for the 1984 water year of $1,065,000. The streamflow-gaging program in Montana has evolved through the years as Federal, State, and local needs for surface-water data have increased. Continuous streamflow records for periods ranging from less than 1 year to more than 90 years have been collected. This report describes phase 1 of a cost-effectiveness study of the streamflow-gaging program in Montana. Evaluation of the program indicates that numerous agencies use the data for studies involving regional hydrology, hydrologic systems, and planning and design. They also use the data for operations of existing hydroelectric and irrigation dams, forecasting flood and seasonal flows, water-quality monitoring, research studies for fish habitat, and other uses such as recreational management. (USGS)

  19. Petrography and petrology of Smoky Butte intrusives, Garfield County, Montana

    USGS Publications Warehouse

    Matson, Robert E.

    1960-01-01

    The Smoky Butte intrusives are located in T. 18 N., R. 36 E. Garfield County, Montana on the extreme eastern edge of the petrographic province of Central Montana. They consist of dikes and plugs arranged in linear, en-echelon pattern with a northeast trend and intrude the Tullock member (Paleocene age) of the Fort Union formation. Extrusive rocks are absent. The rocks are potassium-rich volcanic types showing a disequilibrium mineral assemblage consisting of sanidine, leucite, biotite, olivine, pyroxene, magnetite plus. ilmenite, apatite, calcite, quartz, and a yellowish to dark greenish glassy groundmass. Two chemical analyses of Smoky Butte rocks show high magnesium, potassium, titanium, and phosphorous and low aluminum and sodium content. The two norm calculations show that the rocks are oversaturated with 1.3 and 3.1 per-cent excess silica. Because of the peculiar nature of the Smoky Butte rocks, descriptive names have been applied to them. They are divided into six different types. Three periods of intrusion are proposed for Smoky Butte quarry where three rock types crop out. Other evidence for multiple injection occurs in several multiple dikes. The upper contact of the intrusion is visible on a few plugs and dikes. Smoky Butte rocks show some similarities to the undersaturated potassium-rich rocks of the Highwood and Bearpaw Mountains of Montana, the rocks of the Leucite Hills of Wyoming, and the oversaturated rocks of the West Kimberly District of Australia.

  20. Preliminary assessment report for Fort William Henry Harrison, Montana Army National Guard, Helena, Montana. Installation Restoration Program

    SciTech Connect

    DuWaldt, J.; Meyer, T.

    1993-07-01

    This report presents the results of the preliminary assessment (PA) conducted by Argonne National Laboratory at a Montana Army National Guard (MTARNG) property near Helena, Montana. Preliminary assessments of federal facilities are being conducted to compile the information necessary for completing preremedial activities and to provide a basis for establishing corrective actions in response to releases of hazardous substances. The principal objective of the PA is to characterize the site accurately and determine the need for further action by examining site activities, quantities of hazardous substances present, and potential pathways by which contamination could affect public health and the environment. This PA satisfies, for the Fort William Henry Harrison property, requirements of the Department of Defense Installation Restoration Program.

  1. Stratigraphy and geologic history of the Montana group and equivalent rocks, Montana, Wyoming, and North and South Dakota

    USGS Publications Warehouse

    Gill, James R.; Cobban, William Aubrey

    1973-01-01

    During Late Cretaceous time a broad north-trending epicontinental sea covered much of the western interior of North America and extended from the Gulf of Mexico to the Arctic Ocean. The sea was bounded on the west by a narrow, unstable, and constantly rising cordillera which extended from Central America to Alaska and which separated the sea from Pacific oceanic waters. The east margin of the sea was bounded by the low-lying stable platform of the central part of the United States.Rocks of the type Montana Group in Montana and equivalent rocks in adjacent States, which consist of eastward-pointing wedges of shallow-water marine and nonmarine strata that enclose westward-pointing wedges of fine-grained marine strata, were deposited in and marginal to this sea. These rocks range in age from middle Santonian to early Maestrichtian and represent a time span of about 14 million years. Twenty-nine distinctive ammonite zones, each with a time span of about half a million years, characterize the marine strata.Persistent beds of bentonite in the transgressive part of the Claggett and Bearpaw Shales of Montana and equivalent rocks elsewhere represent periods of explosive volcanism and perhaps concurrent subsidence along the west shore in the vicinity of the Elkhorn Mountains and the Deer Creek volcanic fields in Montana. Seaward retreat of st randlines, marked by deposition of the Telegraph Creek, Eagle, Judith River, and Fox Hills Formations in Montana and the Mesaverde Formation in Wyoming, may be attributed to uplift in near-coastal areas and to an increase in volcaniclastic rocks delivered to the sea.Rates of transgression and regression determined for the Montana Group in central Montana reveal that the strandline movement was more rapid during times of transgression. The regression of the Telegraph Creek and Eagle strandlines averaged about 50 miles per million years compared with a rate of about 95 miles per million years for the advance of the strand-line during

  2. Precambrian basement geologic map of Montana; an interpretation of aeromagnetic anomalies

    USGS Publications Warehouse

    Sims, P.K.; O'Neill, J. M.; Bankey, Viki; Anderson, E.

    2004-01-01

    Newly compiled aeromagnetic anomaly data of Montana, in conjunction with the known geologic framework of basement rocks, have been combined to produce a new interpretive geologic basement map of Montana. Crystalline basement rocks compose the basement, but are exposed only in the cores of mountain ranges in southwestern Montana. Principal features deduced from the map are: (1) A prominent northeast-trending, 200-km-wide zone of spaced negative anomalies, which extends more than 700 km from southwestern Montana's Beaverhead Mountains to the Canadian border and reflects suturing of the Archean Mexican Hat Block against the Archean Wyoming Province along the Paleoproterozoic Trans-Montana Orogen (new name) at about 1.9-1.8 Ga; (2) North-northwest-trending magnetic lows in northeastern Montana, which reflect the 1.9-1.8 Ga Trans-Hudson Orogen and truncate the older Trans-Montana Zone; and (3) Subtle northwest- and west-trending negative anomalies in central and western Montana, which represent the northernmost segment of brittle-ductile transcurrent faults of the newly recognized Mesoproterozoic Trans-Rocky Mountain fault system. Structures developed in the Proterozoic provided zones of crustal weakness reactivated during younger Proterozoic and Phanerozoic igneous and tectonic activity. For example, the Trans-Montana Zone guided basement involved thrust faulting in southwestern Montana during the Sevier Orogeny. The Boulder Batholith and associated ore deposits and the linear belt of alkaline intrusions to the northeast were localized along a zone of weakness between the Missouri River suture and the Dillon shear zone of the Trans-Montana Orogen. The northwest-trending faults of Trans-Rocky Mountain system outline depocenters for sedimentary rocks in the Belt Basin. This fault system provided zones of weakness that guided Laramide uplifts during basement crustal shortening. Northwest-trending zones have been locally reactivated during Neogene basin-range extension.

  3. Pueraria mirifica Exerts Estrogenic Effects in the Mammary Gland and Uterus and Promotes Mammary Carcinogenesis in Donryu Rats

    PubMed Central

    Kakehashi, Anna; Yoshida, Midori; Tago, Yoshiyuki; Ishii, Naomi; Okuno, Takahiro; Gi, Min; Wanibuchi, Hideki

    2016-01-01

    Pueraria mirifica (PM), a plant whose dried and powdered tuberous roots are now widely used in rejuvenating preparations to promote youthfulness in both men and women, may have major estrogenic influence. In this study, we investigated modifying effects of PM at various doses on mammary and endometrial carcinogenesis in female Donryu rats. Firstly, PM administered to ovariectomized animals at doses of 0.03%, 0.3%, and 3% in a phytoestrogen-low diet for 2 weeks caused significant increase in uterus weight. Secondly, a 4 week PM application to non-operated rats at a dose of 3% after 7,12-dimethylbenz[a]anthracene (DMBA) initiation resulted in significant elevation of cell proliferation in the mammary glands. In a third experiment, postpubertal administration of 0.3% (200 mg/kg body weight (b.w.)/day) PM to 5-week-old non-operated animals for 36 weeks following initiation of mammary and endometrial carcinogenesis with DMBA and N-ethyl-N′-nitro-N-nitrosoguanidine (ENNG), respectively, resulted in significant increase of mammary adenocarcinoma incidence. A significant increase of endometrial atypical hyperplasia multiplicity was also observed. Furthermore, PM at doses of 0.3%, and more pronouncedly, at 1% induced dilatation, hemorrhage and inflammation of the uterine wall. In conclusion, postpubertal long-term PM administration to Donryu rats exerts estrogenic effects in the mammary gland and uterus, and at a dose of 200 mg/kg b.w./day was found to promote mammary carcinogenesis initiated by DMBA. PMID:27827907

  4. Puerariae flos alleviates metabolic diseases in Western diet-loaded, spontaneously obese type 2 diabetic model mice.

    PubMed

    Kubo, Koshi; Shimada, Tsutomu; Onishi, Rei; Tsubata, Masahito; Kamiya, Tomoyasu; Nagamine, Rika; Iizuka, Seiichi; Sai, Yoshimichi; Amagaya, Sakae; Aburada, Masaki; Miyamoto, Ken-ichi

    2012-10-01

    Puerariae flos extract (PFE) has been reported to have many effects, including preventing the development of hangovers, liver protective effects, and an estrogenic effect. In addition, some papers reported that PFE is effective against metabolic diseases, with hypolipidemic and hypoglycemic effects. However, the mechanism underlying such effects remains unclear. For the purpose of clarifying the effect of PFE on metabolic diseases related to the accumulation of visceral fat and to determine the mechanism of such action, TSOD mice, a multifactorial genetic disease animal model that spontaneously develops various metabolic diseases such as obesity and type 2 diabetes, were given a Western diet (WTD) as an environmental factor to prepare a disease model (TSOD-WTD). When TSOD mice were loaded with WTD, it was confirmed that metabolic diseases such as obesity and abnormal glucose/lipid metabolism are aggravated. In contrast, PFE treatment to TSOD-WTD mice was shown to suppress body weight gain and visceral fat accumulation, alleviated the abnormal glucose tolerance and hyperinsulinemia, as well as causing an increase in blood adiponectin. Furthermore, the suppression of liver enlargement was observed in PFE-treated mice, with suppression of fatty degeneration and anti-inflammatory effect. In addition, to clarify the mechanism of the hyperlipidemia-alleviating effects in the liver, we investigated the effect of PFE on the expression of genes involved in cholesterol homeostasis. PFE was associated with a significant increase in gene expression for cholesterol synthesis rate-limiting enzyme HMG-CoA reductase, cholesterol catabolization enzyme Cyp7A1, bile salt export pump adenosine triphosphate-binding cassette transporter B11, and low-density lipoprotein receptor involved in cholesterol uptake. The above results suggest that PFE acts to alleviate the effects of various metabolic diseases based on the accumulation of visceral adipose tissue, including obesity, diabetes

  5. Effects of Pueraria lobata Root Ethanol Extract on Adipogenesis and Lipogenesis During 3T3-L1 Differentiation into Adipocytes.

    PubMed

    Lee, Chae Myoung; Yoon, Mi Sook; Kim, Young Chul

    2015-06-01

    We evaluated the inhibitory effect of Pueraria lobata root ethanol extract (PLREE) on lipid accumulation during 3T3-L1 differentiation to adipocytes by measuring the intracellular expression of adipogenic, lipogenic, and lipolytic markers and lipid accumulation. The total polyphenol and flavonoid content of PLREE were 47 and 29 mg/g, respectively. The electron donating capacity of PLREE at 1,000 μg/mL was 48.8%. Treatment of 3T3-L1 preadipocytes with 100, 250, or 500 μg/mL PLREE for 8 days dose-dependently promoted the differentiation of 3T3-L1 cells. In contrast, the lipid content of PLREE-treated cells was significantly reduced by 7.8% (p < 0.05), 35.6% (p < 0.001), and 42.2% (p < 0.001) following treatment with 100, 250, and 500 μg/mL PLREE, respectively, as compared to differentiated control cells. PLREE upregulated peroxisome proliferator-activated receptor γ mRNA and protein, and sterol regulator element-binding protein-1c mRNA levels, but did not affect CCAAT/enhancer binding-protein β and α mRNA levels. PLREE also downregulated acetyl-CoA carboxylase mRNA and protein, fatty acid synthase (FAS) protein, and leptin mRNA levels, but did not affect FAS mRNA expression. PLREE upregulated adipose triglyceride lipase mRNA and protein expression, and hormone-sensitive lipase (HSL) protein expression, but did not affect HSL mRNA expression. In conclusion, we found that PLREE enhanced adipogenesis, but reduced lipogenesis, resulting in decreased lipid accumulation in 3T3-L1 cells.

  6. High performance enzyme-linked immunosorbent assay for determination of miroestrol, a potent phytoestrogen from Pueraria candollei.

    PubMed

    Yusakul, Gorawit; Udomsin, Orapin; Juengwatanatrakul, Thaweesak; Tanaka, Hiroyuki; Chaichantipyuth, Chaiyo; Putalun, Waraporn

    2013-06-27

    Pueraria candollei associated preparation is widely applied in folk Thai medicine for rejuvenating purpose in aged people, which correlated with its pharmacological activities reported by pre-clinical and clinical trials. Therefore, standardized products of this plant are needed by consumers and health care personnel. Miroestrol, a potent and stable phytoestrogen in P. candollei, exhibited potential to be biomarker for quality control of P. candollei samples in research or industrial levels. Indirect competitive enzyme-linked immunosorbant assay (ELISA) for miroestrol determination was developed and validated by using polyclonal antibody from rabbit immunization. The polyclonal antibody recognized specifically to miroestrol, which exhibited cross-reactivity to deoxymiroestrol and isomiroestrol with 6.68% and 1.05%, respectively. The linearity range of measurement was 0.73-3000 ng mL(-1), which coefficient of variation (CV) of both intra- and inter-plate determination was less than 5%. With spiked samples of known amount miroestrol, the percentages of recovery were 98.80-104.37% and 98.31-106.69% in P. candollei and its involved product samples, respectively. Validated ELISA was comparable with published HPLC method (R(2)=0.9996) (Yusakul et al.) in samples with various miroestrol contents. For application, the P. candollei involved preparations contained miroestrol 0.695±0.037-12.108±0.285 μg g(-1) dry wt. The developed ELISA was high performance for miroestrol determination, which could be applied for P. candollei quality control in research fields and industrial productions. Copyright © 2013 Elsevier B.V. All rights reserved.

  7. A validated RP-HPLC-UV method for quantitative determination of puerarin in Pueraria tuberosa DC tuber extract.

    PubMed

    Maji, Amal K; Maity, Niladri; Banerji, Pratim; Banerjee, Debdulal

    2012-07-01

    Pueraria tuberosa (Fabaceae) is a well-known medicinal herbs used in Indian traditional medicines. The puerarin is one of the most important bioactive constituent found in the tubers of this plant. Quantitative estimation of bioactive molecules is essential for the purpose of quality control and dose determination of herbal medicines. The study was designed to develop a validated reversed phase high-performance liquid chromatography (RP-HPLC) method for the quantification of puerarin in the tuber extract of P. tuberosa. The RP-HPLC system with Luna C18 (2) 100 Å, 250 × 4.6 mm column was used in this study. The analysis was performed using the mobile phase: 0.1% acetic acid in acetonitrile and 0.1% acetic acid in water (90:10, v/v) under column temperature 25°C. The detection wavelength was set at 254 nm with a flow rate of 1 ml/min. The method validation was performed according to the guidelines of International Conference on Harmonization. The puerarin content of P. tuberosa extract was found to be 9.28 ±0.09%. The calibration curve showed good linearity relationship in the range of 200-1000μg/ml (r (2)>0.99). The LOD and LOQ were 57.12 and 181.26μg/ml, respectively and the average recovery of puerarin was 99.73% ±1.02%. The evaluation of system suitability, precision, robustness and ruggedness parameters were also found to produce satisfactory results. The developed method is very simple and rapid with excellent specificity, accuracy and precision which can be useful for the routine analysis and quantitative estimation of puerarin in plant extracts and formulations.

  8. Anti-osteoporotic effects of Pueraria candollei var. mirifica on bone mineral density and histomorphometry in estrogen-deficient rats.

    PubMed

    Suthon, Sarocha; Jaroenporn, Sukanya; Charoenphandhu, Narattaphol; Suntornsaratoon, Panan; Malaivijitnond, Suchinda

    2016-04-01

    Although it has been clearly shown that Pueraria mirifica and its phytoestrogens can mimic estrogen in preventing bone loss, as osteoporosis is an asymptomatic disease, the therapeutic effects of P. mirifica should be acknowledged. In this study, 6-month-old female rats were ovariectomized, kept for 4 weeks to induce bone loss, divided into five groups, and treated with P. mirifica at doses of 0, 5, 25, and 50 mg/kg BW/day (PM0, PM5, PM25, and PM50 groups, respectively) or 7 mg/kg BW/day of puerarin (PU group) for 12 weeks. Only the trabecular bone mineral densities (BMDs) of tibia metaphysis (at the 12th, 14th, and 16th week) and total and trabecular BMDs of L4 (at the 16th week) of the PM50 group were significantly higher than those of the PM0 group. However, the BMDs of tibia metaphysis and L4 at the 16th week of the study period were kept significantly lower than those of the 0 week, and the BMD was also significantly lower than that of the 4th week for tibia metaphysis. The trabecular bone area (BV/TV), trabecular number (Tb.N), and osteoblast surface (Ob.S/BS) were significantly higher, and trabecular space (Tb.Sp) was significantly lower in the PM50 group, as compared with those of the PM0 group. This study indicates that P. mirifica could be used as an anti-osteoporotic agent for postmenopausal women. Since P. mirifica could mainly retain bone mass at the levels before bone loss is initiated, the use of other anabolic agents in combination with P. mirifica is recommended for osteoporotic patients.

  9. Pueraria mirifica extract and puerarin enhance proliferation and expression of alkaline phosphatase and type I collagen in primary baboon osteoblasts.

    PubMed

    Tiyasatkulkovit, Wacharaporn; Malaivijitnond, Suchinda; Charoenphandhu, Narattaphol; Havill, Lorena M; Ford, Allen L; VandeBerg, John L

    2014-10-15

    Phytoestrogen-rich Pueraria mirifica (PM) tuberous extract is a promising candidate for the development of anti-osteoporosis drugs for postmenopausal women, but its action has never been validated in humans or in non-human primates, which are more closely related to humans than rodents. In vitro study of non-human primate osteoblasts is thus fundamental to prepare for in vivo studies of phytoestrogen effects on primate bone. This study aimed to establish a culture system of baboon primary osteoblasts and to investigate the effects of PM extract and its phytoestrogens on these cells. Primary osteoblasts from adult baboon fibulae exhibited osteoblast characteristics in regard to proliferation, differentiation, mineralization, and estrogen receptor expression. They responded to 17β-estradiol by increased proliferation rate and mRNA levels of alkaline phosphatase (ALP), type I collagen, and osteocalcin. After being exposed for 48 h to 100 μg/ml PM extract, 1000 nM genistein, or 1000 nM puerarin, primary baboon osteoblasts markedly increased the rate of proliferation and mRNA levels of ALP and type I collagen without changes in Runx2, osterix, or osteocalcin expression. PM extract, genistein, and puerarin also decreased the RANKL/OPG ratio, suggesting that they could decrease osteoclast-mediated bone resorption. However, neither PM extract nor its phytoestrogens altered calcium deposition in osteoblast culture. In conclusion, we have established baboon primary osteoblast culture, which is a new tool for bone research and drug discovery. Furthermore, the present results provide substantial support for the potential of PM extract and its phytoestrogens to be developed as therapeutic agents against bone fragility.

  10. A validated RP-HPLC-UV method for quantitative determination of puerarin in Pueraria tuberosa DC tuber extract

    PubMed Central

    Maji, Amal K.; Maity, Niladri; Banerji, Pratim; Banerjee, Debdulal

    2012-01-01

    Background: Pueraria tuberosa (Fabaceae) is a well-known medicinal herbs used in Indian traditional medicines. The puerarin is one of the most important bioactive constituent found in the tubers of this plant. Quantitative estimation of bioactive molecules is essential for the purpose of quality control and dose determination of herbal medicines. The study was designed to develop a validated reversed phase high-performance liquid chromatography (RP-HPLC) method for the quantification of puerarin in the tuber extract of P. tuberosa. Materials and Methods: The RP-HPLC system with Luna C18 (2) 100 Å, 250 × 4.6 mm column was used in this study. The analysis was performed using the mobile phase: 0.1% acetic acid in acetonitrile and 0.1% acetic acid in water (90:10, v/v) under column temperature 25°C. The detection wavelength was set at 254 nm with a flow rate of 1 ml/min. The method validation was performed according to the guidelines of International Conference on Harmonization. Results: The puerarin content of P. tuberosa extract was found to be 9.28 ±0.09%. The calibration curve showed good linearity relationship in the range of 200-1000μg/ml (r2>0.99). The LOD and LOQ were 57.12 and 181.26μg/ml, respectively and the average recovery of puerarin was 99.73% ±1.02%. The evaluation of system suitability, precision, robustness and ruggedness parameters were also found to produce satisfactory results. Conclusions: The developed method is very simple and rapid with excellent specificity, accuracy and precision which can be useful for the routine analysis and quantitative estimation of puerarin in plant extracts and formulations. PMID:23781483

  11. Enhanced Oral Bioavailability of Pueraria Flavones by a Novel Solid Self-microemulsifying Drug Delivery System (SMEDDS) Dropping Pills.

    PubMed

    Guan, Qingxiang; Zhang, Guangyuan; Sun, Shilin; Fan, Hongbo; Sun, Cheng; Zhang, Shaoyuan

    2016-05-01

    To improve bioavailability of pueraria flavones (PF), a self-microemulsifying drug delivery system (SMEDDS) dropping pills composed of PF, Crodamol GTCC, Maisine 35-1, Cremophor RH 40, 1,2-propylene glycol and polyethylene glycol 6000 (PEG6000) was developed. Particle size, zeta potential, morphology and in vitro drug release were investigated, respectively. Pharmacokinetics, bioavailability of PF-SMEDDS dropping pills and commercial Yufengningxin dropping pills were also evaluated and compared in rats. Puerarin treated as the representative component of PF was analyzed. Dynamic light scattering showed the ability of PF-SMEDDS dropping pills to form a nanoemulsion droplet size in aqueous media. The type of media showed no significant effects on the release rate of PF. PF-SMEDDS dropping pills were able to improve the in vitro release rate of PF, and the in vitro release of these dropping pills was significantly faster than that of Yufengningxin dropping pills. There was a dramatic difference between the mean value of t1/2, peak concentration (Cmax), the area of concentration-time curve from 0 to 6 h (AUC0-6 h) of PF-SMEDDS dropping pills and that of commercial Yufengningxin dropping pills. A pharmacokinetic study showed that the bioavailability of PF was greatly enhanced by PF-SMEDDS dropping pills. The value of Cmax and relative bioavailability of PF-SMEDDS dropping pills were dramatically improved by an average of 1.69- and 2.36-fold compared with that of Yufengningxin dropping pills after gavage administration, respectively. It was concluded that bioavailability of PF was greatly improved and that PF-SMEDDS dropping pills might be an encouraging strategy to enhance the oral bioavailability of PF.

  12. Efficacy and Safety of Pueraria lobata Extract in Gray Hair Prevention: A Randomized, Double-Blind, Placebo-Controlled Study

    PubMed Central

    Jo, Seong Jin; Shin, Hyoseung; Paik, Seung Hwan; Na, Sun Jae; Jin, Yingji; Park, Won Seok; Kim, Su Na

    2013-01-01

    Background Graying of hair-a sign of aging-raises cosmetic concerns. Individuals with gray hair often look older than others their age; therefore, some dye their hair for aesthetic purposes. However, hair colorants can induce many problems including skin irritation, allergic reaction and hair-breakage. Objective This randomized, double-blind clinical trial was performed in order to examine the effects of APHG-1001, a compound including an extract from Pueraria lobata, on graying hair. Methods A total of 44 female subjects were randomly treated with either APHG-1001 or placebo twice daily for 24 weeks. Using the phototrichogram analysis, a count of newly developed gray hair was estimated. Investigator assessment and subject self-assessment were also performed in order to evaluate the efficacy of the compound. Results The mean number of newly developed gray hair at 24 weeks was 6.3/cm2 in the APHG-1001 group and 11.4/cm2 in the placebo group; the difference was statistically significant (p<0.05). However, the investigator assessment and subject self-assessment did not show any significant change in the gross appearance of hair grayness by the end of the study. No severe adverse events in either group were observed. Moreover, the incidence of adverse events did not differ between the groups. Conclusion This clinical trial revealed that APHG-1001, which contains an extract of P. lobata, could prevent the development of new gray hair without any remarkable adverse effects. Thus, it can be considered as a viable treatment option for the prevention of gray hair. PMID:23717015

  13. In vivo metabolic tracking of 14C-radiolabelled isoflavones in kudzu (Pueraria lobata) and red clover (Trifolium pratense) extracts.

    PubMed

    Mun, Jonathan G; Grannan, Michael D; Lachcik, Pamela J; Reppert, Adam; Yousef, Gad G; Rogers, Randy B; Janle, Elsa M; Weaver, Connie M; Lila, Mary Ann

    2009-11-01

    Absorption, distribution and elimination of 14C-labelled isoflavone-containing extracts from kudzu (Pueraria lobata) root culture and red clover (Trifolium pratense) cell culture were investigated in an in vivo rat model. The predominant isoflavones in the kudzu extract were the glycosides puerarin, daidzin and malonyl daidzin, while in the red clover extract, the major isoflavones were formononetin and its derivatives, genistein and biochanin A, with radioactivities of 3.770 and 7.256 MBq/g, respectively. Male Sprague-Dawley rats, implanted with a jugular catheter and a subcutaneous ultrafiltrate probe, were orally administered with 14C-labelled isoflavone extracts from either kudzu or clover cell cultures. Serum, interstitial fluid (ISF), urine and faeces were collected using a Culex Automated Blood Collection System for 24 h. Analysis of bone tissues revealed that radiolabel accumulated in the femur, tibia and vertebrae at 0.04, 0.03 and 0.01 % of the administered dose, respectively, in both kudzu and red clover treatments. The liver accumulated the greatest concentration of radiolabel among the tissues tested, at 1.99 and 1.54 % of the administered kudzu and red clover extracts, respectively. Serum and ISF analysis showed that both extracts were rapidly absorbed, distributed in various tissues, and largely eliminated in the urine and faeces. Urine and faeces contained 8.53 and 9.06 % of the kudzu dose, respectively, and 3.60 and 5.64 % of the red clover dose, respectively. Serum pharmacokinetics suggest that extracts from kudzu may undergo enterohepatic circulation.

  14. The Effect of Pueraria Lobata/Rehmannia Glutinosa and Exercise on Fatty Acid Transporters Expression in Ovariectomized Rats Skeletal Muscles

    PubMed Central

    Kim, Hye Jin; Yoon, Hae Min; Kwon, Oran; Lee, Won Jun

    2016-01-01

    [Purpose] Pueraria lobata/rehmannia glutinosa (PR) and exercise have been receiving a lot of attention from postmenopausal women, as a result of the side effects of estrogen replacement therapy. However, the effects of PR and exercise on fatty acid transporters (FATPs), which play essential role in fatty acid transport, have not been studied. In this study, we evaluated the effects of PR and aerobic exercise on FATP1, FABPpm and FAT/CD36 expression in ovariectomized rat skeletal muscles. [Methods] Sixty rats were randomly divided into 6 groups: (1)HSV; high fat diet (HFD)+sedentary+vehicle, (2)HSP; HFD+sedentary+PR, (3)HSH; HFD+sedentary+17β-estradiol, (4)HEV; HFD+exercise+vehicle, (5) HEP; HFD+exercise+PR, (6)HEH; HFD+exercise+17β-estradiol. Exercise consisted of treadmill exercise (1-4th week: 15 m/min for 30 min, 5-8th week: 18 m/min for 40 min, 5 times/week). [Results] Exercise does not alter FATP1 and FAT/CD36 gene levels in soleus and plantaris muscles. In contrast, exercise had main effect on up-regulation of FABPpm mRNA expression in both muscles. However, FABPpm level was not increased by exercise combined with treatments, indicative of no additive effects of PR or hormone on FABPpm gene expression. On the other hand, immunohistochemistry result showed that translocation of FATPs proteins to plasma membrane were higher in PR, exercise groups, and exercise combined with PR groups in both muscles. [Conclusion] These result showed that aerobic exercise and PR may help increase fat-oxidation through the induction of FABPpm, a muscle specific transporter, in OVX rat skeletal muscles. In addition, FABPpm expression is possibly regulated post-transcriptionally in exercise, or pre-translationally in PR. PMID:27757385

  15. Feeding value of hays of tropical forage legumes in pigs: Vigna unguiculata, Psophocarpus scandens, Pueraria phaseoloides and Stylosanthes guianensis.

    PubMed

    Kambashi, Bienvenu; Boudry, Christelle; Picron, Pascale; Kiatoko, Honoré; Bindelle, Jérôme

    2014-12-01

    The effects of four tropical forage legume hays (Vigna unguiculata, Psophocarpus scandens, Pueraria phaseoloides and Stylosanthes guianensis) on voluntary feed intake (VFI) and their nutritive value were studied in growing pigs using a corn-soybean meal-based diet containing varying proportions of forage legume hays (0, 10, 20 and 40 % or 0, 12.5 and 25 % for VFI and nutritive value determination, respectively). There was no difference in VFI between species (P > 0.20), but a linear response to forage inclusion level (P < 0.05) was observed decreasing from 126 for 0 % to approximately 84 g/kg of body weight for the 40 % forage diets, except for V. unguiculata, where the response was quadratic (P = 0.01). All four forage species linearly decreased the total tract apparent digestibility (TTAD) from 0.76 to 0.61, 0.80 to 0.68, 0.54 to 0.40 and 0.58 to 0.31 except for S. guianensis (0.44) for DM, N, NDF and N retention, respectively. Differences in digestibility (P < 0.05) between species were also observed. Due to their negative influence on the overall digestibility, the contribution of hays should not exceed 12.5 %, except for S. guianensis, in which N retention remained quite high (0.44) at the highest inclusion level (25 %). P. phaseoloides hay should be avoided in pigs as it combines the lowest VFI with the lowest nutrient digestibility.

  16. Site Investigation Report. Volume 1. 120th Fighter Interceptor Group, Montana Air National Guard, International Airport, Great Falls, Montana

    DTIC Science & Technology

    1992-02-01

    typical soil profile, the surface layer is approximately 7 inches thick and consists of a dark grayish-brown fine sandy loam and an underlying material of...HPLC High performance liquid chromatography HAZWRAP Hazardous Waste Remedial Actions Program IRP Installation Restoration Program MANG Montana Air...light brown andgrayish-brown fine sandy to silty clay loam. Total thickness of the soil ranges from 20 inches to greater than 40 inches (Soil

  17. The crude extract from puerariae flower exerts antiobesity and antifatty liver effects in high-fat diet-induced obese mice.

    PubMed

    Kamiya, Tomoyasu; Sameshima-Kamiya, Mayu; Nagamine, Rika; Tsubata, Masahito; Ikeguchi, Motoya; Takagaki, Kinya; Shimada, Tsutomu; Aburada, Masaki

    2012-01-01

    Kudzu, a leguminous plant, has long been used in folk medicine. In particular, its flowers are used in Japanese and Chinese folk medicine for treating hangovers. We focused on the flower of Kudzu (Puerariae thomsonii), and we previously reported the antiobesity effect of Puerariae thomsonii flower extract (PFE) in humans. In this study, we conducted an animal study to investigate the effect of PFE on visceral fat and hepatic lipid levels in mice with diet-induced obesity. In addition, we focused on gene expression profiles to investigate the antiobesity mechanism of PFE. Male C57BL/6J mice were fed a high-fat diet (HFD) or an HFD supplemented with 5% PFE for 14 days. PFE supplementation significantly reduced body weight and white adipose tissue (WAT) weight. Moreover, in the histological analysis, PFE supplementation improved fatty liver. Hepatic reverse transcription-polymerase chain reaction revealed that PFE supplementation downregulated acetyl-CoA carboxylase expression. For adipose tissue, the expressions of hormone-sensitive lipase in WAT and uncoupling protein 1 in brown adipose tissue (BAT) were significantly upregulated. These results suggest that PFE exerts antiobesity and antifatty liver effects in high-fat diet-induced obese mice through suppressing lipogenesis in the liver, stimulating lipolysis in WAT, and promoting thermogenesis in BAT.

  18. Effects of Puerariae Radix Extract on Endotoxin Receptors and TNF-α Expression Induced by Gut-Derived Endotoxin in Chronic Alcoholic Liver Injury

    PubMed Central

    Peng, Jing-Hua; Cui, Tuan; Sun, Zhao-Lin; Huang, Fu; Chen, Liang; Xu, Lin; Feng, Qin; Hu, Yi-Yang

    2012-01-01

    Kudzu (Pueraria lobata) is one of the earliest medicinal plants used to treat alcohol abuse in traditional Chinese medicine for more than a millennium. However, little is known about its effects on chronic alcoholic liver injury. Therefore, the present study observed the effects of puerariae radix extract (RPE) on chronic alcoholic liver injury as well as Kupffer cells (KCs) activation to release tumor necrosis factor alpha (TNF-α) induced by gut-derived endotoxin in rats and macrophage cell line. RPE was observed to alleviate the pathological changes and lipids deposition in liver tissues as well as the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and hepatic gamma-glutamyl transpeptidase (GGT) activity. Meanwhile, RPE inhibited KCs activation and subsequent hepatic TNF-α expression and downregulated the protein expression of endotoxin receptors, lipopolysaccharide binding protein (LBP), CD14, Toll-like receptor (TLR) 2, and TLR4 in chronic alcohol intake rats. Furthermore, an in vitro study showed that RPE inhibited the expression of TNF-α and endotoxin receptors, CD14 and TLR4, induced by LPS in RAW264.7 cells. In summary, this study demonstrated that RPE mitigated liver damage and lipid deposition induced by chronic alcohol intake in rats, as well as TNF-α release, protein expression of endotoxin receptors in vivo or in vitro. PMID:23133491

  19. Information Technology: Its Impact on and Implications for Education in Montana. A Critical Issues Paper.

    ERIC Educational Resources Information Center

    Kohl, John W.; And Others

    Intended to stimulate discussion by Montana state leaders, this paper examines critical issues raised by the application of new technologies in public education in terms of the challenges they present to the Montana educational system. A statement of each issue is followed by a discussion of its implications. The issues examined relate to the…

  20. Monitoring biological control agents and leafy spurge populations along the Smith River in Montana, USA

    Treesearch

    J. Birdsall; G. Markin; T. Kalaris; J. Runyon

    2013-01-01

    The Smith River originates in west central Montana and flows north approximately 100 miles before joining the Missouri River. The central 60 miles of the river flows through a relatively inaccessible, forested, scenic limestone canyon famous for its trout fishing. Because of its popularity, the area was designated Montana's first and only controlled river, with...