Sample records for pyki hannu nurmesniemi

  1. An Alternative Way of Responding to Powerful Ideas: Notes to Accompany the Poem Entitled "Five Principles of Quality in Narratives of Action Research"

    ERIC Educational Resources Information Center

    Saunders, Lesley

    2007-01-01

    This paper is written in a personal capacity and mainly takes the form of a poem--in five sections--composed as a response to some of the ideas in the article by Hannu Heikkinen and colleagues in this same issue of "Educational Action Research": "Action research as narrative: five principles for validation". The poem takes its…

  2. Phosphoenolpyruvate:glucose phosphotransferase system modification increases the conversion rate during L-tryptophan production in Escherichia coli.

    PubMed

    Liu, Lina; Chen, Sheng; Wu, Jing

    2017-10-01

    Escherichia coli FB-04(pta1), a recombinant L-tryptophan production strain, was constructed in our laboratory. However, the conversion rate (L-tryptophan yield per glucose) of this strain is somewhat low. In this study, additional genes have been deleted in an effort to increase the conversion rate of E. coli FB-04(pta1). Initially, the pykF gene, which encodes pyruvate kinase I (PYKI), was inactivated to increase the accumulation of phosphoenolpyruvate, a key L-tryptophan precursor. The resulting strain, E. coli FB-04(pta1)ΔpykF, showed a slightly higher L-tryptophan yield and a higher conversion rate in fermentation processes. To further improve the conversion rate, the phosphoenolpyruvate:glucose phosphotransferase system (PTS) was disrupted by deleting the ptsH gene, which encodes the phosphocarrier protein (HPr). The levels of biomass, L-tryptophan yield, and conversion rate of this strain, E. coli FB-04(pta1)ΔpykF/ptsH, were especially low during fed-batch fermentation process, even though it achieved a significant increase in conversion rate during shake-flask fermentation. To resolve this issue, four HPr mutations (N12S, N12A, S46A, and S46N) were introduced into the genomic background of E. coli FB-04(pta1)ΔpykF/ptsH, respectively. Among them, the strain harboring the N12S mutation (E. coli FB-04(pta1)ΔpykF-ptsHN12S) showed a prominently increased conversion rate of 0.178 g g -1 during fed-batch fermentation; an increase of 38.0% compared with parent strain E. coli FB-04(pta1). Thus, mutation of the genomic of ptsH gene provided an alternative method to weaken the PTS and improve the efficiency of carbon source utilization.

  3. Frontiers in Numerical Relativity

    NASA Astrophysics Data System (ADS)

    Evans, Charles R.; Finn, Lee S.; Hobill, David W.

    2011-06-01

    Christopher S. Kochanek and Charles R. Evans; 22. Relativistic hydrodynamics James R. Wilson and Grant J. Mathews; 23. Computational dynamics of U(1) gauge strings: probability of reconnection of cosmic strings Richard A. Matzner; 24. Dynamically inhomogenous cosmic nucleosynthesis Hannu Kurki-Suonio; 25. Initial value solutions in planar cosmologies Peter Anninos, Joan Centrella and Richard Matzner; 26. An algorithmic overview of an Einstein solver Roger Ove; 27. A PDE compiler for full-metric numerical relativity Jonathan Thornburg; 28. Numerical evolution on null cones R. Gomez and J. Winicour; 29. Normal modes coupled to gravitational waves in a relativistic star Yasufumi Kojima; 30. Cosmic censorship and numerical relativity Dalia S. Goldwirth, Amos Ori and Tsvi Piran.

  4. Feasibility of Gamified Mobile Service Aimed at Physical Activation in Young Men: Population-Based Randomized Controlled Study (MOPO).

    PubMed

    Leinonen, Anna-Maiju; Pyky, Riitta; Ahola, Riikka; Kangas, Maarit; Siirtola, Pekka; Luoto, Tim; Enwald, Heidi; Ikäheimo, Tiina M; Röning, Juha; Keinänen-Kiukaanniemi, Sirkka; Mäntysaari, Matti; Korpelainen, Raija; Jämsä, Timo

    2017-10-10

    fitness from baseline between the groups, whereas waist circumference was reduced in the most inactive men within the intervention group (P=.01). Among completers with valid physical activity data (n=167), there was a borderline difference in the change in mean daily time spent in moderate to vigorous physical activity between the groups (11.9 min vs -9.1 min, P=.055, linear mixed model). Within the intervention group (n=87), baseline vigorous physical activity was inversely associated with change in moderate to vigorous physical activity during the trial (R=-.382, P=.01). The various functionalities related to physical activity of the gamified tailored mobile service were considered important. However, the compliance was limited. Within the current setup, the mobile service had no effect on anthropometry or fitness, except reduced waist circumference in the most inactive men. Among completers with valid physical activity data, the trial had a borderline positive effect on moderate to vigorous physical activity. Further development is needed to improve the feasibility and adherence of an integrated multifunctional service. Clinicaltrials.gov NCT01376986; http://clinicaltrials.gov/ct2/show/NCT01376986 (Archived by WebCite at http://www.webcitation.org/6tjdmIroA). ©Anna-Maiju Leinonen, Riitta Pyky, Riikka Ahola, Maarit Kangas, Pekka Siirtola, Tim Luoto, Heidi Enwald, Tiina M Ikäheimo, Juha Röning, Sirkka Keinänen-Kiukaanniemi, Matti Mäntysaari, Raija Korpelainen, Timo Jämsä. Originally published in JMIR Mhealth and Uhealth (http://mhealth.jmir.org), 10.10.2017.

  5. Introducing litter quality to the ecosystem model LPJ-GUESS: Effects on short- and long-term soil carbon dynamics

    NASA Astrophysics Data System (ADS)

    Portner, Hanspeter; Wolf, Annett; Rühr, Nadine; Bugmann, Harald

    2010-05-01

    and 2007 [Rühr(2009)] and present soil carbon stocks [Heim et al.(2009)]. Our Results show, that for short-term soil carbon dynamics, e.g. estimates of heterotrophic soil respiration on an annual basis, the inclusion of the dependency on litter quality is not necessary, as the differences are minor only. However, when considering long-term soil carbon dynamics, e.g. simulated estimates of present soil carbon content, the dependency on litter quality shows effect, as there are correlations with specific site factors such as site location and forest type. The inclusion of the dependence on litter quality therefore may be of importance for the projection of future soil carbon dynamics, as forest types may well be altered due to climatic change. References [Heim et al.(2009)] A. Heim, L. Wehrli, W. Eugster, and M.W.I. Schmidt. Effects of sampling design on the probability to detect soil carbon stock changes at the swiss CarboEurope site Lägeren. Geoderma, 149(3-4):347-354, 2009. [Rühr(2009)] Nadine Katrin Rühr. Soil respiration in a mixed mountain forest : environmental drivers and partitioning of component fluxes. PhD thesis, ETH, 2009. [Smith et al.(2001)] Benjamin Smith, I. Colin Prentice, and Martin T. Sykes. Representation of vegetation dynamics in the modelling of terrestrial ecosystems: comparing two contrasting approaches within european climate space. Global Ecology and Biogeography, 10(6):621-637, 2001. [Tuomi et al.(2008)] Mikko Tuomi, Pekka Vanhala, Kristiina Karhu, Hannu Fritze, and Jari Liski. Heterotrophic soil respiration-Comparison of different models describing its temperature dependence. Ecological Modelling, 211(1-2): 182-190, 2008.