Air Force Ni-Cd cell qualification program update

NASA Technical Reports Server (NTRS)

Hall, Steve; Brown, Harry; Collins, G.; Hwang, W.; Bui, Q.

1993-01-01

The generic qualification of aerospace nickel-cadmium cells is discussed. The test program includes the following: all available manufacturers, all available designs, cells from the previous program, and high and low orbit life cycling. It is the purpose of this program to characterize the beginning of life performance.

Results from the DOE Advanced Gas Reactor Fuel Development and Qualification Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

David Petti

2014-06-01

Modular HTGR designs were developed to provide natural safety, which prevents core damage under all design basis accidents and presently envisioned severe accidents. The principle that guides their design concepts is to passively maintain core temperatures below fission product release thresholds under all accident scenarios. This level of fuel performance and fission product retention reduces the radioactive source term by many orders of magnitude and allows potential elimination of the need for evacuation and sheltering beyond a small exclusion area. This level, however, is predicated on exceptionally high fuel fabrication quality and performance under normal operation and accident conditions. Germanymore » produced and demonstrated high quality fuel for their pebble bed HTGRs in the 1980s, but no U.S. manufactured fuel had exhibited equivalent performance prior to the Advanced Gas Reactor (AGR) Fuel Development and Qualification Program. The design goal of the modular HTGRs is to allow elimination of an exclusion zone and an emergency planning zone outside the plant boundary fence, typically interpreted as being about 400 meters from the reactor. To achieve this, the reactor design concepts require a level of fuel integrity that is better than that claimed for all prior US manufactured TRISO fuel, by a few orders of magnitude. The improved performance level is about a factor of three better than qualified for German TRISO fuel in the 1980’s. At the start of the AGR program, without a reactor design concept selected, the AGR fuel program selected to qualify fuel to an operating envelope that would bound both pebble bed and prismatic options. This resulted in needing a fuel form that could survive at peak fuel temperatures of 1250°C on a time-averaged basis and high burnups in the range of 150 to 200 GWd/MTHM (metric tons of heavy metal) or 16.4 to 21.8% fissions per initial metal atom (FIMA). Although Germany has demonstrated excellent performance of TRISO

Desirable Qualifications for Personnel Conducting Educational Program Evaluations and Audits.

ERIC Educational Resources Information Center

Newman, Warren B.

A study was made of professional qualifications for personnel employed as program evaluators and auditors. These qualifications, according to operational or theoretical models, are necessary to assure local school districts of obtaining the services of competent and ethical personnel. Findings of (1) a review of literature, (2) a national survey…

AF Ni-Cd cell qualification program

NASA Technical Reports Server (NTRS)

Hall, Steve; Brown, Harry; Collins, G.; Hwang, Warren

1994-01-01

The present status of the USAF NiCd cell qualification program, which is underway at the Naval Surface Warfare Center-Crane Division, is summarized. The following topics are discussed: overview; background; purpose; stress tests; results for super Ni-Cd; results for SAFT cells; GPS stress test; GPS simulated orbit; and results for gates cells. The discussion is presented in viewgraph format.

49 CFR 236.1041 - Training and qualification program, general.

Code of Federal Regulations, 2010 CFR

2010-10-01

... INSTALLATION, INSPECTION, MAINTENANCE, AND REPAIR OF SIGNAL AND TRAIN CONTROL SYSTEMS, DEVICES, AND APPLIANCES Positive Train Control Systems § 236.1041 Training and qualification program, general. (a) Training program...-critical elements of the railroad's PTC systems, including central office, wayside, or onboard subsystems...

7 CFR 400.55 - Qualification for actual production history coverage program.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 6 2014-01-01 2014-01-01 false Qualification for actual production history coverage... Production History § 400.55 Qualification for actual production history coverage program. (a) The approved... history is certified and T or D-Yields are not provided in the actuarial documents, (2) If actual yield...

Initial results from safety testing of US AGR-2 irradiation test fuel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Morris, Robert Noel; Hunn, John D.; Baldwin, Charles A.

Two cylindrical compacts containing tristructural isotropic (TRISO)-coated particles with kernels that contained a mixture of uranium carbide and uranium oxide (UCO) and two compacts with UO 2-kernel TRISO particles have undergone 1600°C safety testing. These compacts were irradiated in the US Advanced Gas Reactor Fuel Development and Qualification Program's second irradiation test (AGR-2). The time-dependent releases of several radioisotopes ( 110mAg, 134Cs, 137Cs, 154Eu, 155Eu, 90Sr, and 85Kr) were monitored while heating the fuel specimens to 1600°C in flowing helium for 300 h. The UCO compacts behaved similarly to previously reported 1600°C-safety-tested UCO compacts from the AGR-1 irradiation. No failedmore » TRISO or failed SiC were detected (based on krypton and cesium release), and cesium release through intact SiC was very low. Release behavior of silver, europium, and strontium appeared to be dominated by inventory originally released through intact coating layers during irradiation but retained in the compact matrix until it was released during safety testing. Both UO 2 compacts exhibited cesium release from multiple particles whose SiC failed during the safety test. Europium and strontium release from these two UO 2 compacts appeared to be dominated by release from the particles with failed SiC. Silver release was characteristically like the release from the UCO compacts in that an initial release of the majority of silver trapped in the matrix occurred during ramping to 1600°C. However, additional silver release was observed later in the safety testing due to the UO 2 TRISO with failed SiC. Failure of the SiC layer in the UO 2 fuel appears to have been dominated by CO corrosion, as opposed to the palladium degradation observed in AGR-1 UCO fuel.« less

Initial results from safety testing of US AGR-2 irradiation test fuel

DOE PAGES

Morris, Robert Noel; Hunn, John D.; Baldwin, Charles A.; ...

2017-08-18

Two cylindrical compacts containing tristructural isotropic (TRISO)-coated particles with kernels that contained a mixture of uranium carbide and uranium oxide (UCO) and two compacts with UO 2-kernel TRISO particles have undergone 1600°C safety testing. These compacts were irradiated in the US Advanced Gas Reactor Fuel Development and Qualification Program's second irradiation test (AGR-2). The time-dependent releases of several radioisotopes ( 110mAg, 134Cs, 137Cs, 154Eu, 155Eu, 90Sr, and 85Kr) were monitored while heating the fuel specimens to 1600°C in flowing helium for 300 h. The UCO compacts behaved similarly to previously reported 1600°C-safety-tested UCO compacts from the AGR-1 irradiation. No failedmore » TRISO or failed SiC were detected (based on krypton and cesium release), and cesium release through intact SiC was very low. Release behavior of silver, europium, and strontium appeared to be dominated by inventory originally released through intact coating layers during irradiation but retained in the compact matrix until it was released during safety testing. Both UO 2 compacts exhibited cesium release from multiple particles whose SiC failed during the safety test. Europium and strontium release from these two UO 2 compacts appeared to be dominated by release from the particles with failed SiC. Silver release was characteristically like the release from the UCO compacts in that an initial release of the majority of silver trapped in the matrix occurred during ramping to 1600°C. However, additional silver release was observed later in the safety testing due to the UO 2 TRISO with failed SiC. Failure of the SiC layer in the UO 2 fuel appears to have been dominated by CO corrosion, as opposed to the palladium degradation observed in AGR-1 UCO fuel.« less

49 CFR 229.317 - Training and qualification program.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Training and qualification program. 229.317 Section 229.317 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD LOCOMOTIVE SAFETY STANDARDS Locomotive Electronics § 229...

49 CFR 229.317 - Training and qualification program.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Training and qualification program. 229.317 Section 229.317 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD LOCOMOTIVE SAFETY STANDARDS Locomotive Electronics § 229...

49 CFR 229.317 - Training and qualification program.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false Training and qualification program. 229.317 Section 229.317 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD LOCOMOTIVE SAFETY STANDARDS Locomotive Electronics § 229...

RL10A-3-3B high mixture ratio qualification program

NASA Technical Reports Server (NTRS)

Vogel, T.; Varella, D.; Smith, C.

1987-01-01

The results of the high mixture ratio qualification testing of the RL10 engine for the Shuttle/Centaur program are presented. The objective of the engine qualification test was to demonstrate the suitability of the RL10A-3-3B engine for space vehicle flight by subjecting it to the testing specified in RL10A-3-3B Model Specification Number 2295 dated February 1986. The applicable section of the specification is presented. Due to payload volume advantages which can be achieved by increasing the operating mixture ratio of the RL10, a decision was made to qualify the engine to run at a higher mixture ratio. A program was created to qualify the RL10 engine for operation at 15,000 pounds thrust and a nominal 6.0 to 1 mixture ratio. This model of the engine was designated the RL10A-3-3B. The qualification program included three test series as follows: (1) hardware durability and limits test in which the engine completed 23 firings and 4605.7 seconds with 1588.7 seconds at less than 6.6 mixture ratio; (2) preliminary qualification test in which the engine completed 26 firings and 5750 seconds; and (3) qualification test in which the engine completed 26 hot firings and 5693.4 seconds with 905.9 seconds at 6.7 mixture ratio. Several changes in engine hardware were required for operation of the RL10A-3-3B engine in the Space Shuttle which include a duel pressure switch ignition, an oxidizer flow control, and helium plumbing changes.

Establishment of an unrelated umbilical cord blood bank qualification program: ensuring quality while meeting Food and Drug Administration vendor qualification requirements.

PubMed

Rabe, Fran; Kadidlo, Diane; Van Orsow, Lisa; McKenna, David

2013-10-01

Qualification of a cord blood bank (CBB) is a complex process that includes evaluation of multiple aspects of donor screening and testing, processing, accreditation and approval by professional cell therapy groups, and results of received cord blood units. The University of Minnesota Medical Center Cell Therapy Laboratory has established a CBB vendor qualification process to ensure the CBB meets established regulatory and quality requirements. The deployed qualification of CBBs is based on retrospective and prospective review of the CBB. Forty-one CBBs were evaluated retrospectively: seven CBBs were disqualified based on failed quality control (QC) results. Eight CBBs did not meet the criteria for retrospective qualification because fewer than 3 cord blood units were received and the CBB was not accredited. As of March 2012, three US and one non-US CBBs have been qualified prospectively. One CBB withdrew from the qualification process after successful completion of the comprehensive survey and subsequent failure of the provided QC unit to pass the minimum criteria. One CBB failed the prospective qualification process based on processing methods that were revealed during the paper portion of the evaluation. A CBB qualification process is necessary for a transplant center to manage the qualification of the large number of CBBs needed to support a umbilical cord blood transplantation program. A transplant center that has utilized cord blood for a number of years before implementation of a qualification process should use a retrospective qualification process along with a prospective process. © 2013 American Association of Blood Banks.

49 CFR 236.921 - Training and qualification program, general.

Code of Federal Regulations, 2010 CFR

2010-10-01

... INSTALLATION, INSPECTION, MAINTENANCE, AND REPAIR OF SIGNAL AND TRAIN CONTROL SYSTEMS, DEVICES, AND APPLIANCES Standards for Processor-Based Signal and Train Control Systems § 236.921 Training and qualification program..., wayside, or onboard subsystems; (2) Persons who dispatch train operations (issue or communicate any...

Quantity of 135I released from the AGR-1, AGR-2, and AGR-3/4 experiments and discovery of 131I at the FPMS traps during the AGR-3/4 experiment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Scates, Dawn M.

2014-09-01

A series of three Advanced Gas Reactor (AGR) experiments have been conducted in the Advanced Test Reactor (ATR) at Idaho National Laboratory (INL). From 2006 through 2014, these experiments supported the development and qualification of the new U.S. tristructural isotropic (TRISO) particle fuel for Very High Temperature Reactors (VHTR). Each AGR experiment consisted of multiple fueled capsules, each plumbed for independent temperature control using a mix of helium and neon gases. The gas leaving a capsule was routed to individual Fission Product Monitor (FPM) detectors. For intact fuel particles, the TRISO particle coatings provide a substantial barrier to fission productmore » release. However, particles with failed coatings, whether because of a minute percentage of initially defective particles, those which fail during irradiation, or those designed to fail (DTF) particles, can release fission products to the flowing gas stream. Because reactive fission product elements like iodine and cesium quickly deposit on cooler capsule components and piping structures as the effluent gas leaves the reactor core, only the noble fission gas isotopes of Kr and Xe tend to reach FPM detectors. The FPM system utilizes High Purity Germanium (HPGe) detectors coupled with a thallium activated sodium iodide NaI(Tl) scintillator. The HPGe detector provides individual isotopic information, while the NaI(Tl) scintillator is used as a gross count rate meter. During irradiation, the 135mXe concentration reaching the FPM detectors is from both direct fission and by decay of the accumulated 135I. About 2.5 hours after irradiation (ten 15.3 minute 135mXe half lives) the directly produced 135mXe has decayed and only the longer lived 135I remains as a source. Decay systematics dictate that 135mXe will be in secular equilibrium with its 135I parent, such that its production rate very nearly equals the decay rate of the parent, and its concentration in the flowing gas stream will appear to

AgrAbility: Frequently Asked Questions

MedlinePlus

... AgrAbility Resources AgrAbility Services Equipment and Vehicle Modifications Financing-Related Matters Other Modifications Other Disability and Agricultural-related questions Main Menu Home About AgrAbility State Projects Directory The Toolbox AT Database Resources Veterans & Beginning ...

45 CFR 2522.950 - What requirements and qualifications apply if my program focuses on supplemental academic support...

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 4 2013-10-01 2013-10-01 false What requirements and qualifications apply if my program focuses on supplemental academic support activities other than tutoring? 2522.950 Section 2522.950... § 2522.950 What requirements and qualifications apply if my program focuses on supplemental academic...

45 CFR 2522.950 - What requirements and qualifications apply if my program focuses on supplemental academic support...

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 4 2012-10-01 2012-10-01 false What requirements and qualifications apply if my program focuses on supplemental academic support activities other than tutoring? 2522.950 Section 2522.950... § 2522.950 What requirements and qualifications apply if my program focuses on supplemental academic...

45 CFR 2522.950 - What requirements and qualifications apply if my program focuses on supplemental academic support...

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 4 2011-10-01 2011-10-01 false What requirements and qualifications apply if my program focuses on supplemental academic support activities other than tutoring? 2522.950 Section 2522.950... § 2522.950 What requirements and qualifications apply if my program focuses on supplemental academic...

45 CFR 2522.950 - What requirements and qualifications apply if my program focuses on supplemental academic support...

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 4 2014-10-01 2014-10-01 false What requirements and qualifications apply if my program focuses on supplemental academic support activities other than tutoring? 2522.950 Section 2522.950... § 2522.950 What requirements and qualifications apply if my program focuses on supplemental academic...

KEY RESULTS FROM IRRADIATION AND POST-IRRADIATION EXAMINATION OF AGR-1 UCO TRISO FUEL

DOE Office of Scientific and Technical Information (OSTI.GOV)

Demkowicz, Paul A.; Hunn, John D.; Petti, David A.

The AGR-1 irradiation experiment was performed as the first test of tristructural isotropic (TRISO) fuel in the US Advanced Gas Reactor Fuel Development and Qualification Program. The experiment consisted of 72 right cylinder fuel compacts containing approximately 3×105 coated fuel particles with uranium oxide/uranium carbide (UCO) fuel kernels. The fuel was irradiated in the Advanced Test Reactor for a total of 620 effective full power days. Fuel burnup ranged from 11.3 to 19.6% fissions per initial metal atom and time average, volume average irradiation temperatures of the individual compacts ranged from 955 to 1136°C. This paper focuses on key resultsmore » from the irradiation and post-irradiation examination, which revealed a robust fuel with excellent performance characteristics under the conditions tested and have significantly improved the understanding of UCO coated particle fuel irradiation behavior within the US program. The fuel exhibited a very low incidence of TRISO coating failure during irradiation and post-irradiation safety testing at temperatures up to 1800°C. Advanced PIE methods have allowed particles with SiC coating failure to be isolated and meticulously examined, which has elucidated the specific causes of SiC failure in these specimens. The level of fission product release from the fuel during irradiation and post-irradiation safety testing has been studied in detail. Results indicated very low release of krypton and cesium through intact SiC and modest release of europium and strontium, while also confirming the potential for significant silver release through the coatings depending on irradiation conditions. Focused study of fission products within the coating layers of irradiated particles down to nanometer length scales has provided new insights into fission product transport through the coating layers and the role various fission products may have on coating integrity. The broader implications of these results and the application

AGR-2: The first irradiation of French HTR fuel in Advanced Test Reactor

DOE Office of Scientific and Technical Information (OSTI.GOV)

T. Lambert; B. Grover; P. Guillermier

AGR-2, the second irradiation of the US program for qualification of the NGNP fuel, is open to international participation within the scope of the Generation IV International Forum. In this frame, it includes in its multi-capsule irradiation rig an irradiation of French HTR fuel manufactured in the CAPRI line (GAIA facility at CEA/Cadarache and AREVA/CERCA compacting line at Romans). The AGR-2 irradiation is designed to place our first fabrications of HTR particles under operating conditions that are representative of ANTARES project while keeping close to the test range of the German fuel as much as possible, which is the referencemore » in terms of irradiation behavior. A few batches of particles and 12 fuel compacts were produced and characterized in 2009 by CEA and CERCA. The fuel main characteristics are in conformity with our specifications and in compliance with INL requirements. The AGR-2 experiment is based on the design and devices used in the first experiment of the AGR program. The design makes it possible to monitor the irradiation conditions and in particular, the temperature, the power and the fission products released from fuel particles. The in pile equipment consists of a multi-capsule device designed to simultaneously irradiate six independent capsules with temperature control. The out-of-core part consists of the equipment for actively controlling temperature and measuring the fission products release on-line. The target conditions for the irradiation experiment were defined with the aim of comparing the results obtained under irradiation with German particles along with the objectives of reaching burn-up and fluence targets to validate the behavior of our fuel in a significant range (15% FIMA – 5 × 1025 n/m2 at 600 EFPD with centerline fuel temperature about 1100 degrees C). These conditions have to be representative of ANTARES project characteristics. These target conditions were compared with final results from neutron and thermal design

Qualification of Electrical Ground Support Equipment for New Space Programs

NASA Technical Reports Server (NTRS)

SotoToro, Felix A.; Vu, Bruce T.; Hamilton, Mark S.

2011-01-01

With the Space Shuttle program coming to an end, the National Aeronautics and Space Administration (NASA) is moving to a new space flight program that will allow expeditions beyond low earth orbit. The space vehicles required to comply with these missions will be carrying heavy payloads. This implies that the Earth departure stage capabilities must be of higher magnitudes, given the current propulsion technology. The engineering design of the new flight hardware comes with some structural, thermal, propulsion and other subsystems' challenges. Meanwhile, the necessary ground support equipment (GSE) used to test, validate, verify and process the flight hardware must withstand the new program specifications. This paper intends to provide the qualification considerations during implementation of new electrical GSE for space programs. A team of engineers was formed to embark on this task, and facilitate the logistics process and ensure that the electrical, mechanical and fluids subsystems conduct the proper level of testing. Ultimately, each subsystem must certify that each piece of ground support equipment used in the field is capable of withstanding the strenuous vibration, acoustics, environmental, thermal and Electromagnetic Interference (EMf) levels experienced during pre-launch, launch and post-launch activities. The benefits of capturing and sharing these findings will provide technical, cost savings and schedule impacts infon11ation to both the technical and management community. Keywords: Qualification; Testing; Ground Support Equipment; Electromagnetic Interference Testing; Vibration Testing; Acoustic Testing; Power Spectral Density.

14 CFR 121.909 - Approval of Advanced Qualification Program.

Code of Federal Regulations, 2010 CFR

2010-01-01

... methodology must incorporate a thorough analysis of the certificate holder's operations, aircraft, line environment and job functions. All AQP qualification and continuing qualification curriculums must integrate.... (ii) Initial job task listing. (iii) Instructional systems development methodology. (iv) Qualification...

Disciplining the Disciplines? How Qualification Schemes Are Written up at Study Program Level in Norwegian Higher Education

ERIC Educational Resources Information Center

Friedrich, Philipp E.; Prøitz, Tine S.; Stensaker, Bjørn

2016-01-01

Qualification frameworks are spreading rapidly, not least in Europe following the introduction of the European Qualification Framework. The impact of such frameworks are contested, and the article contributes to this debate by analyzing how a selected group of different study programs in Norwegian higher education is adapting to the newly launched…

76 FR 9376 - Proposed Extension of Existing Information Collection on Qualification/Certification Program and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-17

... Physical Fitness AGENCY: Mine Safety and Health Administration, Department of Labor. ACTION: Notice of... 77.107-1 on Qualification/Certification Program and Man Hoist Operators Physical Fitness. DATES: All...

Sample results from the integrated salt disposition program macrobatch 6 tank 21H qualifications MST solids sample

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peters, T. B.

2013-02-26

Savannah River National Laboratory (SRNL) performed experiments on qualification material for use in the Integrated Salt Disposition Program (ISDP) Batch 6 processing. As part of this qualification work, SRNL performed an Actinide Removal Process (ARP) test. From this test, the residual monosodium titanate (MST) was analyzed for radionuclide uptake. The results of these analyses are reported and are within historical precedent.

X-ray Analysis of Defects and Anomalies in AGR-5/6/7 TRISO Particles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Helmreich, Grant W.; Hunn, John D.; Skitt, Darren J.

2017-06-01

Coated particle fuel batches J52O-16-93164, 93165, 93166, 93168, 93169, 93170, and 93172 were produced by Babcock and Wilcox Technologies (BWXT) for possible selection as fuel for the Advanced Gas Reactor Fuel Development and Qualification (AGR) Program’s AGR-5/6/7 irradiation test in the Idaho National Laboratory (INL) Advanced Test Reactor (ATR), or may be used for other tests. Each batch was coated in a 150-mm-diameter production-scale fluidized-bed chemical vapor deposition (CVD) furnace. Tristructural isotropic (TRISO) coatings were deposited on 425-μm-nominal-diameter spherical kernels from BWXT lot J52R-16-69317 containing a mixture of 15.4%-enriched uranium carbide and uranium oxide (UCO), with the exception of Batchmore » 93164, which used similar kernels from BWXT lot J52L-16-69316. The TRISO-coatings consisted of a ~50% dense carbon buffer layer with 100-μmnominal thickness, a dense inner pyrolytic carbon (IPyC) layer with 40-μm-nominal thickness, a silicon carbide (SiC) layer with 35-μm-nominal thickness, and a dense outer pyrolytic carbon (OPyC) layer with 40-μm-nominal thickness. Each coated particle batch was sieved to upgrade the particles by removing over-sized and under-sized material, and the upgraded batch was designated by appending the letter A to the end of the batch number (e.g., 93164A). Secondary upgrading by sieving was performed on the upgraded batches to remove specific anomalies identified during analysis for Defective IPyC, and the upgraded batches were designated by appending the letter B to the end of the batch number (e.g., 93165B). Following this secondary upgrading, coated particle composite J52R-16-98005 was produced by BWXT as fuel for the AGR Program’s AGR-5/6/7 irradiation test in the INL ATR. This composite was comprised of coated particle fuel batches J52O-16-93165B, 93168B, 93169B, and 93170B.« less

Peter Agre and Aquaporin Water Channels

Science.gov Websites

as a postdoctoral fellow in cell biology. Dr. Agre joined the faculty in 1984 and has spent most of Hopkins Malaria Research Institute (JHMRI) in January, 2008. 'Agre will remain a professor of cell biology

Findings from the Supersonic Qualification Program of the Mars Science Laboratory Parachute System

NASA Technical Reports Server (NTRS)

Sengupta, Anita; Steltzner, Adam; Witkowski, Allen; Candler, Graham; Pantano, Carlos

2009-01-01

In 2012, the Mars Science Laboratory Mission (MSL) will deploy NASA's largest extra-terrestrial parachute, a technology integral to the safe landing of its advanced robotic explorer on the surface. The supersonic parachute system is a mortar deployed 21.5 m disk-gap-band (DGB) parachute, identical in geometric scaling to the Viking era DGB parachutes of the 1970's. The MSL parachute deployment conditions are Mach 2.3 at a dynamic pressure of 750 Pa. The Viking Balloon Launched Decelerator Test (BLDT) successfully demonstrated a maximum of 700 Pa at Mach 2.2 for a 16.1 m DGB parachute in its AV4 flight. All previous Mars deployments have derived their supersonic qualification from the Viking BLDT test series, preventing the need for full scale high altitude supersonic testing. The qualification programs for Mars Pathfinder, Mars Exploration Rover, and Phoenix Scout Missions were all limited to subsonic structural qualification, with supersonic performance and survivability bounded by the BLDT qualification. The MSL parachute, at the edge of the supersonic heritage deployment space and 33% larger than the Viking parachute, accepts a certain degree of risk without addressing the supersonic environment in which it will deploy. In addition, MSL will spend up to 10 seconds above Mach 1.5, an aerodynamic regime that is associated with a known parachute instability characterized by significant canopy projected area fluctuation and dynamic drag variation. This aerodynamic instability, referred to as "area oscillations" by the parachute community has drag performance, inflation stability, and structural implications, introducing risk to mission success if not quantified for the MSL parachute system. To minimize this risk and as an alternative to a prohibitively expensive high altitude test program, a multi-phase qualification program using computation simulation validated by subscale test was developed and implemented for MSL. The first phase consisted of 2% of fullscale

Comparison of fission product release predictions using PARFUME with results from the AGR-1 safety tests

DOE Office of Scientific and Technical Information (OSTI.GOV)

Collin, Blaise P.; Petti, David A.; Demkowicz, Paul A.

Safety tests were conducted on fuel compacts from AGR-1, the first irradiation experiment of the Advanced Gas Reactor (AGR) Fuel Development and Qualification program, at temperatures ranging from 1600 to 1800 °C to determine fission product release at temperatures that bound reactor accident conditions. The PARFUME (PARticle FUel ModEl) code was used to predict the release of fission products silver, cesium, strontium, and krypton from fuel compacts containing tristructural isotropic (TRISO) coated particles during 15 of these safety tests. Comparisons between PARFUME predictions and post-irradiation examination results of the safety tests were conducted on two types of AGR-1 compacts: compactsmore » containing only intact particles and compacts containing one or more particles whose SiC layers failed during safety testing. In both cases, PARFUME globally over-predicted the experimental release fractions by several orders of magnitude: more than three (intact) and two (failed SiC) orders of magnitude for silver, more than three and up to two orders of magnitude for strontium, and up to two and more than one orders of magnitude for krypton. The release of cesium from intact particles was also largely over-predicted (by up to five orders of magnitude) but its release from particles with failed SiC was only over-predicted by a factor of about 3. These over-predictions can be largely attributed to an over-estimation of the diffusivities used in the modeling of fission product transport in TRISO-coated particles. The integral release nature of the data makes it difficult to estimate the individual over-estimations in the kernel or each coating layer. Nevertheless, a tentative assessment of correction factors to these diffusivities was performed to enable a better match between the modeling predictions and the safety testing results. The method could only be successfully applied to silver and cesium. In the case of strontium, correction factors could not be assessed

Comparison of fission product release predictions using PARFUME with results from the AGR-1 safety tests

DOE PAGES

Collin, Blaise P.; Petti, David A.; Demkowicz, Paul A.; ...

2016-04-07

Safety tests were conducted on fuel compacts from AGR-1, the first irradiation experiment of the Advanced Gas Reactor (AGR) Fuel Development and Qualification program, at temperatures ranging from 1600 to 1800 °C to determine fission product release at temperatures that bound reactor accident conditions. The PARFUME (PARticle FUel ModEl) code was used to predict the release of fission products silver, cesium, strontium, and krypton from fuel compacts containing tristructural isotropic (TRISO) coated particles during 15 of these safety tests. Comparisons between PARFUME predictions and post-irradiation examination results of the safety tests were conducted on two types of AGR-1 compacts: compactsmore » containing only intact particles and compacts containing one or more particles whose SiC layers failed during safety testing. In both cases, PARFUME globally over-predicted the experimental release fractions by several orders of magnitude: more than three (intact) and two (failed SiC) orders of magnitude for silver, more than three and up to two orders of magnitude for strontium, and up to two and more than one orders of magnitude for krypton. The release of cesium from intact particles was also largely over-predicted (by up to five orders of magnitude) but its release from particles with failed SiC was only over-predicted by a factor of about 3. These over-predictions can be largely attributed to an over-estimation of the diffusivities used in the modeling of fission product transport in TRISO-coated particles. The integral release nature of the data makes it difficult to estimate the individual over-estimations in the kernel or each coating layer. Nevertheless, a tentative assessment of correction factors to these diffusivities was performed to enable a better match between the modeling predictions and the safety testing results. The method could only be successfully applied to silver and cesium. In the case of strontium, correction factors could not be assessed

10 CFR 26.85 - Collector qualifications and responsibilities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 1 2011-01-01 2011-01-01 false Collector qualifications and responsibilities. 26.85 Section 26.85 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Collecting Specimens for Testing § 26.85 Collector qualifications and responsibilities. (a) Urine collector qualifications. Urine...

10 CFR 26.85 - Collector qualifications and responsibilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Collector qualifications and responsibilities. 26.85 Section 26.85 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Collecting Specimens for Testing § 26.85 Collector qualifications and responsibilities. (a) Urine collector qualifications. Urine...

10 CFR 26.85 - Collector qualifications and responsibilities.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 1 2014-01-01 2014-01-01 false Collector qualifications and responsibilities. 26.85 Section 26.85 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Collecting Specimens for Testing § 26.85 Collector qualifications and responsibilities. (a) Urine collector qualifications. Urine...

10 CFR 26.85 - Collector qualifications and responsibilities.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 1 2013-01-01 2013-01-01 false Collector qualifications and responsibilities. 26.85 Section 26.85 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Collecting Specimens for Testing § 26.85 Collector qualifications and responsibilities. (a) Urine collector qualifications. Urine...

10 CFR 26.85 - Collector qualifications and responsibilities.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 1 2012-01-01 2012-01-01 false Collector qualifications and responsibilities. 26.85 Section 26.85 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Collecting Specimens for Testing § 26.85 Collector qualifications and responsibilities. (a) Urine collector qualifications. Urine...

Key results from irradiation and post-irradiation examination of AGR-1 UCO TRISO fuel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Demkowicz, Paul A.; Hunn, John D.; Petti, David A.

The AGR-1 irradiation experiment was performed as the first test of tristructural isotropic (TRISO) fuel in the US Advanced Gas Reactor Fuel Development and Qualification Program. The experiment consisted of 72 right cylinder fuel compacts containing approximately 3 × 105 coated fuel particles with uranium oxide/uranium carbide (UCO) fuel kernels. The fuel was irradiated in the Advanced Test Reactor for a total of 620 effective full power days. Fuel burnup ranged from 11.3 to 19.6% fissions per initial metal atom and time-average, volume-average irradiation temperatures of the individual compacts ranged from 955 to 1136 °C. This paper focuses on keymore » results from the irradiation and post-irradiation examination, which revealed a robust fuel with excellent performance characteristics under the conditions tested and have significantly improved the understanding of UCO coated particle fuel irradiation behavior. The fuel exhibited zero TRISO coating failures (failure of all three dense coating layers) during irradiation and post-irradiation safety testing at temperatures up to 1700 °C. Advanced PIE methods have allowed particles with SiC coating failure that were discovered to be present in a very-low population to be isolated and meticulously examined, which has elucidated the specific causes of SiC failure in these specimens. The level of fission product release from the fuel during irradiation and post-irradiation safety testing has been studied in detail. Results indicated very low release of krypton and cesium through intact SiC and modest release of europium and strontium, while also confirming the potential for significant silver release through the coatings depending on irradiation conditions. Focused study of fission products within the coating layers of irradiated particles down to nanometer length scales has provided new insights into fission product transport through the coating layers and the role various fission products may have on coating

Key results from irradiation and post-irradiation examination of AGR-1 UCO TRISO fuel

DOE PAGES

Demkowicz, Paul A.; Hunn, John D.; Petti, David A.; ...

2017-09-10

The AGR-1 irradiation experiment was performed as the first test of tristructural isotropic (TRISO) fuel in the US Advanced Gas Reactor Fuel Development and Qualification Program. The experiment consisted of 72 right cylinder fuel compacts containing approximately 3 × 105 coated fuel particles with uranium oxide/uranium carbide (UCO) fuel kernels. The fuel was irradiated in the Advanced Test Reactor for a total of 620 effective full power days. Fuel burnup ranged from 11.3 to 19.6% fissions per initial metal atom and time-average, volume-average irradiation temperatures of the individual compacts ranged from 955 to 1136 °C. This paper focuses on keymore » results from the irradiation and post-irradiation examination, which revealed a robust fuel with excellent performance characteristics under the conditions tested and have significantly improved the understanding of UCO coated particle fuel irradiation behavior. The fuel exhibited zero TRISO coating failures (failure of all three dense coating layers) during irradiation and post-irradiation safety testing at temperatures up to 1700 °C. Advanced PIE methods have allowed particles with SiC coating failure that were discovered to be present in a very-low population to be isolated and meticulously examined, which has elucidated the specific causes of SiC failure in these specimens. The level of fission product release from the fuel during irradiation and post-irradiation safety testing has been studied in detail. Results indicated very low release of krypton and cesium through intact SiC and modest release of europium and strontium, while also confirming the potential for significant silver release through the coatings depending on irradiation conditions. Focused study of fission products within the coating layers of irradiated particles down to nanometer length scales has provided new insights into fission product transport through the coating layers and the role various fission products may have on coating

Targeting agr- and agr-Like Quorum Sensing Systems for Development of Common Therapeutics to Treat Multiple Gram-Positive Bacterial Infections

PubMed Central

Gray, Brian; Hall, Pamela; Gresham, Hattie

2013-01-01

Invasive infection by the Gram-positive pathogen Staphylococcus aureus is controlled by a four gene operon, agr that encodes a quorum sensing system for the regulation of virulence. While agr has been well studied in S. aureus, the contribution of agr homologues and analogues in other Gram-positive pathogens is just beginning to be understood. Intriguingly, other significant human pathogens, including Clostridium perfringens, Listeria monocytogenes, and Enterococcus faecalis contain agr or analogues linked to virulence. Moreover, other significant human Gram-positive pathogens use peptide based quorum sensing systems to establish or maintain infection. The potential for commonality in aspects of these signaling systems across different species raises the prospect of identifying therapeutics that could target multiple pathogens. Here, we review the status of research into these agr homologues, analogues, and other peptide based quorum sensing systems in Gram-positive pathogens as well as the potential for identifying common pathways and signaling mechanisms for therapeutic discovery. PMID:23598501

24 CFR 200.935 - Administrator qualifications and procedures for HUD building products certification programs.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Administrator qualifications and procedures for HUD building products certification programs. 200.935 Section 200.935 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY...

The U.S. Navy’s Consultant Development and Qualification Program: Origin and Issues.

DTIC Science & Technology

1984-03-01

Areasoe Senior Master I Capability Intern Consultant Consultant Consultant I MARKETING Strategy 1--------------- 21 Ijplementation 1 --------- I...SENIOR MASTER INTERN CONSULTANT CONSULTANT CONSULTANT A. MARKETING A.1 Develop Marketing Strategy Program 1 11 A.2 Implement Marketing Program 1 1 1 2 A.3...Qualification Criteria for: Degree of PROFICIENCY LT 0. D. EFFORT SEP 1980 SEP 1983 Date Reported PRD 1 2 3 4 A. MARKETING A.1 Develop Marketing Strategy (1, 1

Advanced Electron Microscopy and Micro analytical technique development and application for Irradiated TRISO Coated Particles from the AGR-1 Experiment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Van Rooyen, Isabella Johanna; Lillo, Thomas Martin; Wen, Haiming

2017-01-01

A series of up to seven irradiation experiments are planned for the Advanced Gas Reactor (AGR) Fuel Development and Quantification Program, with irradiation completed at the Advanced Test Reactor (ATR) at Idaho National Laboratory (INL) for the first experiment (i.e., AGR-1) in November 2009 for an effective 620 full power days. The objective of the AGR-1 experiment was primarily to provide lessons learned on the multi-capsule test train design and to provide early data on fuel performance for use in fuel fabrication process development and post-irradiation safety testing data at high temperatures. This report describes the advanced microscopy and micro-analysismore » results on selected AGR-1 coated particles.« less

45 CFR 2522.950 - What requirements and qualifications apply if my program focuses on supplemental academic support...

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 4 2010-10-01 2010-10-01 false What requirements and qualifications apply if my program focuses on supplemental academic support activities other than tutoring? 2522.950 Section 2522.950... support activities other than tutoring? (a) If your program does not involve tutoring as defined in § 2522...

Options and Risk for Qualification of Electric Propulsion System

NASA Technical Reports Server (NTRS)

Bailey, Michelle; Daniel, Charles; Cook, Steve (Technical Monitor)

2002-01-01

Electric propulsion vehicle systems envelop a wide range of propulsion alternatives including solar and nuclear, which present unique circumstances for qualification. This paper will address the alternatives for qualification of electric propulsion spacecraft systems. The approach taken will be to address the considerations for qualification at the various levels of systems definition. Additionally, for each level of qualification the system level risk implications will be developed. Also, the paper will explore the implications of analysis verses test for various levels of systems definition, while retaining the objectives of a verification program. The limitations of terrestrial testing will be explored along with the risk and implications of orbital demonstration testing. The paper will seek to develop a template for structuring of a verification program based on cost, risk and value return. A successful verification program should establish controls and define objectives of the verification compliance program. Finally the paper will seek to address the political and programmatic factors, which may impact options for system verification.

Democratic Dialogue as a Process to Inform Public Policy: Reconceptualizing a Supervisory Officer's Qualification Program

ERIC Educational Resources Information Center

Smith, Deirdre M.; Qua-Hiansen, Jessica

2015-01-01

An exploration of the collaborative reconceptualization of a provincial Supervisory Officer's Qualification Program (SOQP) through the use of dialogic approaches is the focus of this inquiry. The stories, perspectives, and lived experiences of supervisory officers, principals, teachers, parents, students, and members of the public in Ontario were…

Impact of accessory gene regulator (agr) dysfunction on vancomycin pharmacodynamics among Canadian community and health-care associated methicillin-resistant Staphylococcus aureus

PubMed Central

2011-01-01

Background The accessory gene regulator (agr) is a quorum sensing cluster of genes which control colonization and virulence in Staphylococcus aureus. We evaluated agr function in community- (CA) and healthcare-associated (HA) MRSA, to compare the pharmacodynamics and bactericidal activity of vancomycin against agr functional and dysfunctional HA-MRSA and CA-MRSA. Methods 40 clinical isolates of MRSA from the Canadian Nosocomial Infection Surveillance Program were evaluated for delta-haemolysin production, as a surrogate marker of agr function. Time kill experiments were performed for vancomycin at 0 to 64 times the MIC against an initial inoculum of 106 and 108 cfu/ml of agr functional and dysfunctional CA-MRSA and HA-MRSA and these data were fit to a hill-type pharmacodynamic model. Results 15% isolates were agr dysfunctional, which was higher among HA-MRSA (26.3%) versus CA-MRSA (4.76%). Against a low initial inoculum of 106 cfu/ml of CA-MRSA, vancomycin pharmacodynamics were similar among agr functional and dysfunctional strains. However, against a high initial inoculum of 108 cfu/ml, killing activity was notably attenuated against agr dysfunctional CA-MRSA (USA400) and HA-MRSA (USA100). CA-MRSA displayed a 20.0 fold decrease in the maximal reduction in bacterial counts (Emax) which was 3.71 log10 CFU/ml for agr functional vs. 2.41 log10 CFU/ml for agr dysfunctional MRSA (p = 0.0007). Conclusions Dysfunction in agr was less common among CA-MRSA vs. HA-MRSA. agr dysfunction demonstrated an impact on vancomycin bactericidal activity and pharmacodynamics against a high initial inoculum of CA-MRSA and HA-MRSA, which may have implications for optimal antimicrobial therapy against persistent, difficult to treat MRSA infections. PMID:21599878

Impact of accessory gene regulator (agr) dysfunction on vancomycin pharmacodynamics among Canadian community and health-care associated methicillin-resistant Staphylococcus aureus.

PubMed

Tsuji, Brian T; MacLean, Robert D; Dresser, Linda D; McGavin, Martin J; Simor, Andrew E

2011-05-20

The accessory gene regulator (agr) is a quorum sensing cluster of genes which control colonization and virulence in Staphylococcus aureus. We evaluated agr function in community- (CA) and healthcare-associated (HA) MRSA, to compare the pharmacodynamics and bactericidal activity of vancomycin against agr functional and dysfunctional HA-MRSA and CA-MRSA. 40 clinical isolates of MRSA from the Canadian Nosocomial Infection Surveillance Program were evaluated for delta-haemolysin production, as a surrogate marker of agr function. Time kill experiments were performed for vancomycin at 0 to 64 times the MIC against an initial inoculum of 10(6) and 10(8) cfu/ml of agr functional and dysfunctional CA-MRSA and HA-MRSA and these data were fit to a hill-type pharmacodynamic model. 15% isolates were agr dysfunctional, which was higher among HA-MRSA (26.3%) versus CA-MRSA (4.76%). Against a low initial inoculum of 10(6) cfu/ml of CA-MRSA, vancomycin pharmacodynamics were similar among agr functional and dysfunctional strains. However, against a high initial inoculum of 10(8) cfu/ml, killing activity was notably attenuated against agr dysfunctional CA-MRSA (USA400) and HA-MRSA (USA100). CA-MRSA displayed a 20.0 fold decrease in the maximal reduction in bacterial counts (Emax) which was 3.71 log(10) CFU/ml for agr functional vs. 2.41 log(10) CFU/ml for agr dysfunctional MRSA (p = 0.0007). Dysfunction in agr was less common among CA-MRSA vs. HA-MRSA. agr dysfunction demonstrated an impact on vancomycin bactericidal activity and pharmacodynamics against a high initial inoculum of CA-MRSA and HA-MRSA, which may have implications for optimal antimicrobial therapy against persistent, difficult to treat MRSA infections.

45 CFR 1306.21 - Staff qualification requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 4 2012-10-01 2012-10-01 false Staff qualification requirements. 1306.21 Section 1306.21 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE OF HUMAN DEVELOPMENT... Requirements § 1306.21 Staff qualification requirements. Head Start programs must comply with section 648A of...

45 CFR 1306.21 - Staff qualification requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 4 2014-10-01 2014-10-01 false Staff qualification requirements. 1306.21 Section 1306.21 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE OF HUMAN DEVELOPMENT... Requirements § 1306.21 Staff qualification requirements. Head Start programs must comply with section 648A of...

45 CFR 1306.21 - Staff qualification requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 4 2010-10-01 2010-10-01 false Staff qualification requirements. 1306.21 Section 1306.21 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE OF HUMAN DEVELOPMENT... Requirements § 1306.21 Staff qualification requirements. Head Start programs must comply with section 648A of...

45 CFR 1306.21 - Staff qualification requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 4 2011-10-01 2011-10-01 false Staff qualification requirements. 1306.21 Section 1306.21 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE OF HUMAN DEVELOPMENT... Requirements § 1306.21 Staff qualification requirements. Head Start programs must comply with section 648A of...

45 CFR 1306.21 - Staff qualification requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 4 2013-10-01 2013-10-01 false Staff qualification requirements. 1306.21 Section 1306.21 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE OF HUMAN DEVELOPMENT... Requirements § 1306.21 Staff qualification requirements. Head Start programs must comply with section 648A of...

AGR2, an Endoplasmic Reticulum Protein, Is Secreted into the Gastrointestinal Mucus

PubMed Central

Bergström, Joakim H.; Berg, Katarina A.; Rodríguez-Piñeiro, Ana M.; Stecher, Bärbel; Johansson, Malin E. V.; Hansson, Gunnar C.

2014-01-01

The MUC2 mucin is the major constituent of the two mucus layers in colon. Mice lacking the disulfide isomerase-like protein Agr2 have been shown to be more susceptible to colon inflammation. The Agr2−/− mice have less filled goblet cells and were now shown to have a poorly developed inner colon mucus layer. We could not show AGR2 covalently bound to recombinant MUC2 N- and C-termini as have previously been suggested. We found relatively high concentrations of Agr2 in secreted mucus throughout the murine gastrointestinal tract, suggesting that Agr2 may play extracellular roles. In tissue culture (CHO-K1) cells, AGR2 is normally not secreted. Replacement of the single Cys in AGR2 with Ser (C81S) allowed secretion, suggesting that modification of this Cys might provide a mechanism for circumventing the KTEL endoplasmic reticulum retention signal. In conclusion, these results suggest that AGR2 has both intracellular and extracellular effects in the intestine. PMID:25111734

25 CFR 36.75 - What qualifications must homeliving staff possess?

Code of Federal Regulations, 2011 CFR

2011-04-01

... 25 Indians 1 2011-04-01 2011-04-01 false What qualifications must homeliving staff possess? 36.75... Homeliving Programs Staffing § 36.75 What qualifications must homeliving staff possess? (a) Homeliving staff... must at a minimum have an associate's degree no later than 2008. (3) Homeliving Program Staff Must have...

25 CFR 36.75 - What qualifications must homeliving staff possess?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false What qualifications must homeliving staff possess? 36.75... Homeliving Programs Staffing § 36.75 What qualifications must homeliving staff possess? (a) Homeliving staff... must at a minimum have an associate's degree no later than 2008. (3) Homeliving Program Staff Must have...

25 CFR 36.75 - What qualifications must homeliving staff possess?

Code of Federal Regulations, 2014 CFR

2014-04-01

... 25 Indians 1 2014-04-01 2014-04-01 false What qualifications must homeliving staff possess? 36.75... Homeliving Programs Staffing § 36.75 What qualifications must homeliving staff possess? (a) Homeliving staff... must at a minimum have an associate's degree no later than 2008. (3) Homeliving Program Staff Must have...

Bladder cancer cells secrete while normal bladder cells express but do not secrete AGR2

DOE PAGES

Ho, Melissa E.; Quek, Sue -Ing; True, Lawrence D.; ...

2016-02-15

Anterior gradient 2 (AGR2) is a cancer-associated secreted protein found predominantly in adenocarcinomas. Given its ubiquity in solid tumors, cancer-secreted AGR2 could be a useful biomarker in urine or blood for early detection. Normal organs express AGR2 and might also secrete AGR2, which would impact on the utility of AGR2 as a cancer biomarker. Uniform AGR2 expression is found in the normal bladder urothelium. Little AGR2 is, however, secreted by the urothelial cells as no measurable amounts could be detected in urine. The urinary proteomes of healthy people contain no listing for AGR2. The blood proteomes also contain no significantmore » peptide counts for AGR2 suggesting that little urothelial secretion into capillaries of the lamina propria. Expression is lost in urothelial carcinoma, but 25% primary tumors retained AGR2 expression in a cohort of lymph node positive cases. AGR2 is secreted by the urothelial carcinoma cells as urinary AGR2 was measured in the voided urine of 25% of the cases analyzed in a cohort of cancer vs. non-cancer urine, which matched the frequency of AGR2-positive urothelial carcinoma. Since cancer cells secrete AGR2 while normal cells do not, its measurement in body fluids could be used to indicate tumor presence. In addition to secretion, AGR2 is also localized to the cell surface. Thus, secretion/cell surface localization of AGR2 is pecific to cancer while expression itself is not. Lastly, since AGR2 is found in many solid tumor types, this tumor-associated antigen constitutes a highly promising therapeutic target.« less

Bladder cancer cells secrete while normal bladder cells express but do not secrete AGR2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ho, Melissa E.; Quek, Sue -Ing; True, Lawrence D.

Anterior gradient 2 (AGR2) is a cancer-associated secreted protein found predominantly in adenocarcinomas. Given its ubiquity in solid tumors, cancer-secreted AGR2 could be a useful biomarker in urine or blood for early detection. Normal organs express AGR2 and might also secrete AGR2, which would impact on the utility of AGR2 as a cancer biomarker. Uniform AGR2 expression is found in the normal bladder urothelium. Little AGR2 is, however, secreted by the urothelial cells as no measurable amounts could be detected in urine. The urinary proteomes of healthy people contain no listing for AGR2. The blood proteomes also contain no significantmore » peptide counts for AGR2 suggesting that little urothelial secretion into capillaries of the lamina propria. Expression is lost in urothelial carcinoma, but 25% primary tumors retained AGR2 expression in a cohort of lymph node positive cases. AGR2 is secreted by the urothelial carcinoma cells as urinary AGR2 was measured in the voided urine of 25% of the cases analyzed in a cohort of cancer vs. non-cancer urine, which matched the frequency of AGR2-positive urothelial carcinoma. Since cancer cells secrete AGR2 while normal cells do not, its measurement in body fluids could be used to indicate tumor presence. In addition to secretion, AGR2 is also localized to the cell surface. Thus, secretion/cell surface localization of AGR2 is pecific to cancer while expression itself is not. Lastly, since AGR2 is found in many solid tumor types, this tumor-associated antigen constitutes a highly promising therapeutic target.« less

Historical flight qualifications of space nuclear systems

NASA Astrophysics Data System (ADS)

Bennett, Gary L.

1997-01-01

An overview is presented of the qualification programs for the general-purpose heat source radioisotope thermoelectric generators (GPHS-RTGs) as developed for the Galileo and Ulysses missions; the SNAP-10A space reactor; the Nuclear Engine for Rocket Vehicle Applications (NERVA); the F-1 chemical rocket engine used on the Saturn-V Apollo lunar missions; and the Space Shuttle Main Engines (SSMEs). Some similarities and contrasts between the qualification testing employed on these five programs will be noted. One common thread was that in each of these successful programs there was an early focus on component and subsystem tests to uncover and correct problems.

Comparison of silver, cesium, and strontium release predictions using PARFUME with results from the AGR-1 irradiation experiment

DOE PAGES

Collin, Blaise P.; Petti, David A.; Demkowicz, Paul A.; ...

2015-08-22

Here, the PARFUME (PARticle FUel ModEl) code was used to predict the release of fission products silver, cesium, and strontium from tristructural isotropic coated fuel particles and compacts during the first irradiation experiment (AGR-1) of the Advanced Gas Reactor Fuel Development and Qualification program. The PARFUME model for the AGR-1 experiment used the fuel compact volume average temperature for each of the 620 days of irradiation to calculate the release of silver, cesium, and strontium from a representative particle for a select number of AGR-1 compacts. Post-irradiation examination measurements provided data on release of these fission products from fuel compactsmore » and fuel particles, and retention of silver in the compacts outside of the silicon carbide (SiC) layer. PARFUME-predicted fractional release of silver, cesium, and strontium was determined and compared to the PIE measurements. For silver, comparisons show a trend of over-prediction at low burnup and under-prediction at high burnup. PARFUME has limitations in the modeling of the temporal and spatial distributions of the temperature and burnup across the compacts, which affects the accuracy of its predictions. Nevertheless, the comparisons on silver release lie in the same order of magnitude. Results show an overall over-prediction of the fractional release of cesium by PARFUME. For particles with failed SiC layers, the over-prediction is by a factor of up to 3, corresponding to a potential over-estimation of the diffusivity in uranium oxycarbide (UCO) by a factor of up to 250. For intact particles, whose release is much lower, the over-prediction is by a factor of up to 100, which could be attributed to an over-estimated diffusivity in SiC by about 40% on average. The release of strontium from intact particles is also over-predicted by PARFUME, which also points towards an over-estimated diffusivity of strontium in either SiC or UCO, or possibly both. The measured strontium fractional

Comparison of silver, cesium, and strontium release predictions using PARFUME with results from the AGR-1 irradiation experiment

NASA Astrophysics Data System (ADS)

Collin, Blaise P.; Petti, David A.; Demkowicz, Paul A.; Maki, John T.

2015-11-01

The PARFUME (PARticle FUel ModEl) code was used to predict the release of fission products silver, cesium, and strontium from tristructural isotropic coated fuel particles and compacts during the first irradiation experiment (AGR-1) of the Advanced Gas Reactor Fuel Development and Qualification program. The PARFUME model for the AGR-1 experiment used the fuel compact volume average temperature for each of the 620 days of irradiation to calculate the release of silver, cesium, and strontium from a representative particle for a select number of AGR-1 compacts. Post-irradiation examination (PIE) measurements provided data on release of these fission products from fuel compacts and fuel particles, and retention of silver in the compacts outside of the silicon carbide (SiC) layer. PARFUME-predicted fractional release of silver, cesium, and strontium was determined and compared to the PIE measurements. For silver, comparisons show a trend of over-prediction at low burnup and under-prediction at high burnup. PARFUME has limitations in the modeling of the temporal and spatial distributions of the temperature and burnup across the compacts, which affects the accuracy of its predictions. Nevertheless, the comparisons on silver release lie in the same order of magnitude. Results show an overall over-prediction of the fractional release of cesium by PARFUME. For particles with failed SiC layers, the over-prediction is by a factor of up to 3, corresponding to a potential over-estimation of the diffusivity in uranium oxycarbide (UCO) by a factor of up to 250. For intact particles, whose release is much lower, the over-prediction is by a factor of up to 100, which could be attributed to an over-estimated diffusivity in SiC by about 40% on average. The release of strontium from intact particles is also over-predicted by PARFUME, which also points towards an over-estimated diffusivity of strontium in either SiC or UCO, or possibly both. The measured strontium fractional release

30 CFR 75.155 - Qualified hoisting engineer; qualifications.

Code of Federal Regulations, 2010 CFR

2010-07-01

...-hoisting engineer; or (2) If a State has no program for qualifying persons as electric-hoisting engineers... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Qualified hoisting engineer; qualifications. 75... Persons § 75.155 Qualified hoisting engineer; qualifications. (a)(1) A person is a qualified hoisting...

30 CFR 75.155 - Qualified hoisting engineer; qualifications.

Code of Federal Regulations, 2012 CFR

2012-07-01

...-hoisting engineer; or (2) If a State has no program for qualifying persons as electric-hoisting engineers... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Qualified hoisting engineer; qualifications. 75... Persons § 75.155 Qualified hoisting engineer; qualifications. (a)(1) A person is a qualified hoisting...

30 CFR 75.155 - Qualified hoisting engineer; qualifications.

Code of Federal Regulations, 2013 CFR

2013-07-01

...-hoisting engineer; or (2) If a State has no program for qualifying persons as electric-hoisting engineers... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Qualified hoisting engineer; qualifications. 75... Persons § 75.155 Qualified hoisting engineer; qualifications. (a)(1) A person is a qualified hoisting...

30 CFR 75.155 - Qualified hoisting engineer; qualifications.

Code of Federal Regulations, 2011 CFR

2011-07-01

...-hoisting engineer; or (2) If a State has no program for qualifying persons as electric-hoisting engineers... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Qualified hoisting engineer; qualifications. 75... Persons § 75.155 Qualified hoisting engineer; qualifications. (a)(1) A person is a qualified hoisting...

30 CFR 75.155 - Qualified hoisting engineer; qualifications.

Code of Federal Regulations, 2014 CFR

2014-07-01

...-hoisting engineer; or (2) If a State has no program for qualifying persons as electric-hoisting engineers... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Qualified hoisting engineer; qualifications. 75... Persons § 75.155 Qualified hoisting engineer; qualifications. (a)(1) A person is a qualified hoisting...

Survival of Listeria monocytogenes in Soil Requires AgrA-Mediated Regulation

PubMed Central

Vivant, Anne-Laure; Garmyn, Dominique; Gal, Laurent; Hartmann, Alain

2015-01-01

In a recent paper, we demonstrated that inactivation of the Agr system affects the patterns of survival of Listeria monocytogenes (A.-L. Vivant, D. Garmyn, L. Gal, and P. Piveteau, Front Cell Infect Microbiol 4:160, http://dx.doi.org/10.3389/fcimb.2014.00160). In this study, we investigated whether the Agr-mediated response is triggered during adaptation in soil, and we compared survival patterns in a set of 10 soils. The fate of the parental strain L. monocytogenes L9 (a rifampin-resistant mutant of L. monocytogenes EGD-e) and that of a ΔagrA deletion mutant were compared in a collection of 10 soil microcosms. The ΔagrA mutant displayed significantly reduced survival in these biotic soil microcosms, and differential transcriptome analyses showed large alterations of the transcriptome when AgrA was not functional, while the variations in the transcriptomes between the wild type and the ΔagrA deletion mutant were modest under abiotic conditions. Indeed, in biotic soil environments, 578 protein-coding genes and an extensive repertoire of noncoding RNAs (ncRNAs) were differentially transcribed. The transcription of genes coding for proteins involved in cell envelope and cellular processes, including the phosphotransferase system and ABC transporters, and proteins involved in resistance to antimicrobial peptides was affected. Under sterilized soil conditions, the differences were limited to 86 genes and 29 ncRNAs. These results suggest that the response regulator AgrA of the Agr communication system plays important roles during the saprophytic life of L. monocytogenes in soil. PMID:26002901

Acceptance Test Data for the AGR-5/6/7 Irradiation Test Fuel Composite Defective IPyC Fraction and Pyrocarbon Anisotropy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Helmreich, Grant W.; Hunn, John D.; Skitt, Darren J.

Coated particle composite J52R-16-98005 was produced by Babcock and Wilcox Technologies (BWXT) as fuel for the Advanced Gas Reactor Fuel Development and Qualification (AGR) Program’s AGR-5/6/7 irradiation test in the Idaho National Laboratory (INL) Advanced Test Reactor (ATR). This composite was comprised of four coated particle fuel batches J52O-16-93165B (26%), 93168B (26%), 93169B (24%), and 93170B (24%), chosen based on the Quality Control (QC) data acquired for each individual candidate AGR-5/6/7 batch. Each batch was coated in a 150-mm-diameter production-scale fluidized-bed chemical vapor deposition (CVD) furnace. Tristructural isotropic (TRISO) coatings were deposited on 425-μm-nominal-diameter spherical kernels from BWXT Lot J52R-16-69317more » containing a mixture of 15.5%-enriched uranium carbide and uranium oxide (UCO). The TRISO coatings consisted of four consecutive CVD layers: a ~50% dense carbon buffer layer with 100-μm-nominal thickness, a dense inner pyrolytic carbon (IPyC) layer with 40-μm-nominal thickness, a silicon carbide (SiC) layer with 35-μm-nominal thickness, and a dense outer pyrolytic carbon (OPyC) layer with 40-μm-nominal thickness. The TRISO-coated particle batches were sieved to upgrade the particles by removing over-sized and under-sized material, and the upgraded batches were designated by appending the letter A to the end of the batch number (e.g., 93165A). Secondary upgrading by sieving was performed on the A-designated batches to remove particles with missing or very-thin buffer layers that were identified during previous analysis of the individual batches for defective IPyC, as reported in the acceptance test data report for the AGR-5/6/7 production batches [Hunn et al. 2017]. The additionally-upgraded batches were designated by appending the letter B to the end of the batch number (e.g., 93165B).« less

PIE on Safety-Tested Loose Particles from Irradiated Compact 4-4-2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hunn, John D.; Gerczak, Tyler J.; Morris, Robert Noel

2016-04-01

Post-irradiation examination (PIE) is being performed in support of tristructural isotropic (TRISO) coated particle fuel development and qualification for High Temperature Gas-cooled Reactors (HTGRs). This work is sponsored by the Department of Energy Office of Nuclear Energy (DOE-NE) through the Advanced Reactor Technologies (ART) Office under the Advanced Gas Reactor Fuel Development and Qualification (AGR) Program. The AGR-1 experiment was the first in a series of TRISO fuel irradiation tests initiated in 2006. The AGR-1 TRISO particles and fuel compacts were fabricated at Oak Ridge National Laboratory (ORNL) in 2006 using laboratory-scale equipment and irradiated for 3 years in themore » Advanced Test Reactor (ATR) at Idaho National Laboratory (INL) to demonstrate and evaluate fuel performance under HTGR irradiation conditions. Post-irradiation examination was performed at INL and ORNL to study how the fuel behaved during irradiation, and to test fuel performance during exposure to elevated temperatures at or above temperatures that could occur during a depressurized conduction cooldown event. This report summarizes safety testing and post-safety testing PIE conducted at ORNL on loose particles extracted from irradiated AGR-1 Compact 4-4-2.« less

AGR-2 Irradiated Test Train Preliminary Inspection and Disassembly First Look

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ploger, Scott; Demkowciz, Paul; Harp, Jason

2015-05-01

The AGR 2 irradiation experiment began in June 2010 and was completed in October 2013. The test train was shipped to the Materials and Fuels Complex in July 2014 for post-irradiation examination (PIE). The first PIE activities included nondestructive examination of the test train, followed by disassembly of the test train and individual capsules and detailed inspection of the capsule contents, including the fuel compacts and their graphite fuel holders. Dimensional metrology was then performed on the compacts, graphite holders, and steel capsule shells. AGR 2 disassembly and metrology were performed with the same equipment used successfully on AGR 1more » test train components. Gamma spectrometry of the intact test train gave a preliminary look at the condition of the interior components. No evidence of damage to compacts or graphite components was evident from the isotopic and gross gamma scans. Disassembly of the AGR 2 test train and its capsules was conducted rapidly and efficiently by employing techniques refined during the AGR 1 disassembly campaign. Only one major difficulty was encountered while separating the test train into capsules when thermocouples (of larger diameter than used in AGR 1) and gas lines jammed inside the through tubes of the upper capsules, which required new tooling for extraction. Disassembly of individual capsules was straightforward with only a few minor complications. On the whole, AGR 2 capsule structural components appeared less embrittled than their AGR 1 counterparts. Compacts from AGR 2 Capsules 2, 3, 5, and 6 were in very good condition upon removal. Only relatively minor damage or markings were visible using high resolution photographic inspection. Compact dimensional measurements indicated radial shrinkage between 0.8 to 1.7%, with the greatest shrinkage observed on Capsule 2 compacts that were irradiated at higher temperature. Length shrinkage ranged from 0.1 to 0.9%, with by far the lowest axial shrinkage on Capsule 3

Secretion of protein disulphide isomerase AGR2 confers tumorigenic properties

PubMed Central

Fessart, Delphine; Domblides, Charlotte; Avril, Tony; Eriksson, Leif A; Begueret, Hugues; Pineau, Raphael; Malrieux, Camille; Dugot-Senant, Nathalie; Lucchesi, Carlo; Chevet, Eric; Delom, Frederic

2016-01-01

The extracellular matrix (ECM) plays an instrumental role in determining the spatial orientation of epithelial polarity and the formation of lumens in glandular tissues during morphogenesis. Here, we show that the Endoplasmic Reticulum (ER)-resident protein anterior gradient-2 (AGR2), a soluble protein-disulfide isomerase involved in ER protein folding and quality control, is secreted and interacts with the ECM. Extracellular AGR2 (eAGR2) is a microenvironmental regulator of epithelial tissue architecture, which plays a role in the preneoplastic phenotype and contributes to epithelial tumorigenicity. Indeed, eAGR2, is secreted as a functionally active protein independently of its thioredoxin-like domain (CXXS) and of its ER-retention domain (KTEL), and is sufficient, by itself, to promote the acquisition of invasive and metastatic features. Therefore, we conclude that eAGR2 plays an extracellular role independent of its ER function and we elucidate this gain-of-function as a novel and unexpected critical ECM microenvironmental pro-oncogenic regulator of epithelial morphogenesis and tumorigenesis. DOI: http://dx.doi.org/10.7554/eLife.13887.001 PMID:27240165

Historical flight qualifications of space nuclear systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bennett, G.L.

1997-01-01

An overview is presented of the qualification programs for the general-purpose heat source radioisotope thermoelectric generators (GPHS-RTGs) as developed for the Galileo and Ulysses missions; the SNAP-10A space reactor; the Nuclear Engine for Rocket Vehicle Applications (NERVA); the F-1 chemical rocket engine used on the Saturn-V Apollo lunar missions; and the Space Shuttle Main Engines (SSMEs). Some similarities and contrasts between the qualification testing employed on these five programs will be noted. One common thread was that in each of these successful programs there was an early focus on component and subsystem tests to uncover and correct problems. {copyright} {italmore » 1997 American Institute of Physics.}« less

Survival of Listeria monocytogenes in Soil Requires AgrA-Mediated Regulation.

PubMed

Vivant, Anne-Laure; Garmyn, Dominique; Gal, Laurent; Hartmann, Alain; Piveteau, Pascal

2015-08-01

In a recent paper, we demonstrated that inactivation of the Agr system affects the patterns of survival of Listeria monocytogenes (A.-L. Vivant, D. Garmyn, L. Gal, and P. Piveteau, Front Cell Infect Microbiol 4:160, http://dx.doi.org/10.3389/fcimb.2014.00160). In this study, we investigated whether the Agr-mediated response is triggered during adaptation in soil, and we compared survival patterns in a set of 10 soils. The fate of the parental strain L. monocytogenes L9 (a rifampin-resistant mutant of L. monocytogenes EGD-e) and that of a ΔagrA deletion mutant were compared in a collection of 10 soil microcosms. The ΔagrA mutant displayed significantly reduced survival in these biotic soil microcosms, and differential transcriptome analyses showed large alterations of the transcriptome when AgrA was not functional, while the variations in the transcriptomes between the wild type and the ΔagrA deletion mutant were modest under abiotic conditions. Indeed, in biotic soil environments, 578 protein-coding genes and an extensive repertoire of noncoding RNAs (ncRNAs) were differentially transcribed. The transcription of genes coding for proteins involved in cell envelope and cellular processes, including the phosphotransferase system and ABC transporters, and proteins involved in resistance to antimicrobial peptides was affected. Under sterilized soil conditions, the differences were limited to 86 genes and 29 ncRNAs. These results suggest that the response regulator AgrA of the Agr communication system plays important roles during the saprophytic life of L. monocytogenes in soil. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

49 CFR Appendix B to Part 240 - Procedures for Submission and Approval of Locomotive Engineer Qualification Programs

Code of Federal Regulations, 2012 CFR

2012-10-01

... determining the qualifications of each person it permits or requires to operate a locomotive. In designing its...) for train control that are employed, the operational design characteristics of the track and equipment... completion of a course of study, or successful passage of a standardized testing program, the submission must...

49 CFR Appendix B to Part 240 - Procedures for Submission and Approval of Locomotive Engineer Qualification Programs

Code of Federal Regulations, 2013 CFR

2013-10-01

... determining the qualifications of each person it permits or requires to operate a locomotive. In designing its...) for train control that are employed, the operational design characteristics of the track and equipment... completion of a course of study, or successful passage of a standardized testing program, the submission must...

49 CFR Appendix B to Part 240 - Procedures for Submission and Approval of Locomotive Engineer Qualification Programs

Code of Federal Regulations, 2011 CFR

2011-10-01

... determining the qualifications of each person it permits or requires to operate a locomotive. In designing its...) for train control that are employed, the operational design characteristics of the track and equipment... completion of a course of study, or successful passage of a standardized testing program, the submission must...

49 CFR Appendix B to Part 240 - Procedures for Submission and Approval of Locomotive Engineer Qualification Programs

Code of Federal Regulations, 2010 CFR

2010-10-01

... determining the qualifications of each person it permits or requires to operate a locomotive. In designing its...) for train control that are employed, the operational design characteristics of the track and equipment... completion of a course of study, or successful passage of a standardized testing program, the submission must...

49 CFR Appendix B to Part 240 - Procedures for Submission and Approval of Locomotive Engineer Qualification Programs

Code of Federal Regulations, 2014 CFR

2014-10-01

... determining the qualifications of each person it permits or requires to operate a locomotive. In designing its...) for train control that are employed, the operational design characteristics of the track and equipment... completion of a course of study, or successful passage of a standardized testing program, the submission must...

78 FR 76590 - Physical Qualification of Drivers; Standards; Changes to Vision Exemption Program Criteria

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-18

... No. FMCSA-2013-0097] Physical Qualification of Drivers; Standards; Changes to Vision Exemption... CMV drivers for duty, the Agency established a number of physical qualifications for drivers under 49... provisions of the Federal Motor Carrier Safety Regulations, including physical qualification standards...

49 CFR 198.39 - Qualifications for operation of one-call notification system.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 3 2010-10-01 2010-10-01 false Qualifications for operation of one-call...) PIPELINE SAFETY REGULATIONS FOR GRANTS TO AID STATE PIPELINE SAFETY PROGRAMS Adoption of One-Call Damage Prevention Program § 198.39 Qualifications for operation of one-call notification system. A one-call...

Fission Product Inventory and Burnup Evaluation of the AGR-2 Irradiation by Gamma Spectrometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Harp, Jason Michael; Stempien, John Dennis; Demkowicz, Paul Andrew

Gamma spectrometry has been used to evaluate the burnup and fission product inventory of different components from the US Advanced Gas Reactor Fuel Development and Qualification Program's second TRISO-coated particle fuel irradiation test (AGR-2). TRISO fuel in this irradiation included both uranium carbide / uranium oxide (UCO) kernels and uranium oxide (UO 2) kernels. Four of the 6 capsules contained fuel from the US Advanced Gas Reactor program, and only those capsules will be discussed in this work. The inventories of gamma-emitting fission products from the fuel compacts, graphite compact holders, graphite spacers and test capsule shell were evaluated. Thesemore » data were used to measure the fractional release of fission products such as Cs-137, Cs-134, Eu-154, Ce-144, and Ag-110m from the compacts. The fraction of Ag-110m retained in the compacts ranged from 1.8% to full retention. Additionally, the activities of the radioactive cesium isotopes (Cs-134 and Cs-137) have been used to evaluate the burnup of all US TRISO fuel compacts in the irradiation. The experimental burnup evaluations compare favorably with burnups predicted from physics simulations. Predicted burnups for UCO compacts range from 7.26 to 13.15 % fission per initial metal atom (FIMA) and 9.01 to 10.69 % FIMA for UO 2 compacts. Measured burnup ranged from 7.3 to 13.1 % FIMA for UCO compacts and 8.5 to 10.6 % FIMA for UO 2 compacts. Results from gamma emission computed tomography performed on compacts and graphite holders that reveal the distribution of different fission products in a component will also be discussed. Gamma tomography of graphite holders was also used to locate the position of TRISO fuel particles suspected of having silicon carbide layer failures that lead to in-pile cesium release.« less

Fission Product Inventory and Burnup Evaluation of the AGR-2 Irradiation by Gamma Spectrometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Harp, Jason M.; Demkowicz, Paul A.; Stempien, John D.

Gamma spectrometry has been used to evaluate the burnup and fission product inventory of different components from the US Advanced Gas Reactor Fuel Development and Qualification Program's second TRISO-coated particle fuel irradiation test (AGR-2). TRISO fuel in this irradiation included both uranium carbide / uranium oxide (UCO) kernels and uranium oxide (UO2) kernels. Four of the 6 capsules contained fuel from the US Advanced Gas Reactor program, and only those capsules will be discussed in this work. The inventories of gamma-emitting fission products from the fuel compacts, graphite compact holders, graphite spacers and test capsule shell were evaluated. These datamore » were used to measure the fractional release of fission products such as Cs-137, Cs-134, Eu-154, Ce-144, and Ag-110m from the compacts. The fraction of Ag-110m retained in the compacts ranged from 1.8% to full retention. Additionally, the activities of the radioactive cesium isotopes (Cs-134 and Cs-137) have been used to evaluate the burnup of all US TRISO fuel compacts in the irradiation. The experimental burnup evaluations compare favorably with burnups predicted from physics simulations. Predicted burnups for UCO compacts range from 7.26 to 13.15 % fission per initial metal atom (FIMA) and 9.01 to 10.69 % FIMA for UO2 compacts. Measured burnup ranged from 7.3 to 13.1 % FIMA for UCO compacts and 8.5 to 10.6 % FIMA for UO2 compacts. Results from gamma emission computed tomography performed on compacts and graphite holders that reveal the distribution of different fission products in a component will also be discussed. Gamma tomography of graphite holders was also used to locate the position of TRISO fuel particles suspected of having silicon carbide layer failures that lead to in-pile cesium release.« less

Qualification Lab Testing on M1 Abrams Engine Oil Filters

DTIC Science & Technology

2016-11-01

UNCLASSIFIED QUALIFICATION LAB TESTING ON M1 ABRAMS ENGINE OIL FILTERS FINAL REPORT TFLRF No. 483 by Kristi K. Rutta U.S...the originator. UNCLASSIFIED QUALIFICATION LAB TESTING ON M1 ABRAMS ENGINE OIL FILTERS FINAL REPORT TFLRF No. 483 by Kristi K...TITLE AND SUBTITLE Qualification Lab Testing on M1 Abrams Engine Oil Filter 5a. CONTRACT NUMBER W56HZV-15-C-0030 5b. GRANT NUMBER 5c. PROGRAM

42 CFR 438.354 - Qualifications of external quality review organizations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 4 2010-10-01 2010-10-01 false Qualifications of external quality review... HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL ASSISTANCE PROGRAMS MANAGED CARE External Quality Review § 438.354 Qualifications of external quality review organizations. (a) General rule. The State must...

The agr Locus Regulates Virulence and Colonization Genes in Clostridium difficile 027

PubMed Central

Martin, Melissa J.; Clare, Simon; Goulding, David; Faulds-Pain, Alexandra; Barquist, Lars; Browne, Hilary P.; Pettit, Laura; Dougan, Gordon; Lawley, Trevor D.

2013-01-01

The transcriptional regulator AgrA, a member of the LytTR family of proteins, plays a key role in controlling gene expression in some Gram-positive pathogens, including Staphylococcus aureus and Enterococcus faecalis. AgrA is encoded by the agrACDB global regulatory locus, and orthologues are found within the genome of most Clostridium difficile isolates, including the epidemic lineage 027/BI/NAP1. Comparative RNA sequencing of the wild type and otherwise isogenic agrA null mutant derivatives of C. difficile R20291 revealed a network of approximately 75 differentially regulated transcripts at late exponential growth phase, including many genes associated with flagellar assembly and function, such as the major structural subunit, FliC. Other differentially regulated genes include several involved in bis-(3′-5′)-cyclic dimeric GMP (c-di-GMP) synthesis and toxin A expression. C. difficile 027 R20291 agrA mutant derivatives were poorly flagellated and exhibited reduced levels of colonization and relapses in the murine infection model. Thus, the agr locus likely plays a contributory role in the fitness and virulence potential of C. difficile strains in the 027/BI/NAP1 lineage. PMID:23772065

Crystal Structure of AGR_C_4470p from Agrobacterium tumefaciens

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vorobiev,S.; Neely, H.; Seetharaman, J.

2007-01-01

We report here the crystal structure at 2.0 {angstrom} resolution of the AGR{_}C{_}4470p protein from the Gram-negative bacterium Agrobacterium tumefaciens. The protein is a tightly associated dimer, each subunit of which bears strong structural homology with the two domains of the heme utilization protein ChuS from Escherichia coli and HemS from Yersinia enterocolitica. Remarkably, the organization of the AGR{_}C{_}4470p dimer is the same as that of the two domains in ChuS and HemS, providing structural evidence that these two proteins evolved by gene duplication. However, the binding site for heme, while conserved in HemS and ChuS, is not conserved inmore » AGR{_}C{_}4470p, suggesting that it probably has a different function. This is supported by the presence of two homologs of AGR{_}C{_}4470p in E. coli, in addition to the ChuS protein.« less

Functional Reconstitution of Staphylococcus aureus Truncated AgrC Histidine Kinase in a Model Membrane System

PubMed Central

Liu, Baoquan; Wang, Jianfeng; Xiong, Wen; Zhao, Pengchao; Fan, Shengdi

2013-01-01

The integral membrane protein AgrC is a histidine kinase whose sensor domains interact with an autoinducing peptide, resulting in a series of downstream responses. In this study, truncated AgrCTM5-6C and AgrCTM5-6C-GFP with GFP as a reporter gene were produced using a bacterial system. Purified AgrCTM5-6C and AgrCTM5-6C-GFP were reconstituted into liposomes by a detergent-mediated method. To achieve high-yield protein incorporation, we investigated the effect of different detergents on protein reconstitution efficiency. The highest incorporation was found with N,N-dimethyldode-cylamine N-oxide during complete liposome solubilization, which resulted in a yield of 85±5%. The COOH-terminus of the protein AgrCTM5-6C was almost exclusively oriented towards the inside of the vesicles. AgrCTM5-6C in proteoliposomes exhibited approximately a 6-fold increase in constitutive activity compared with AgrCTM5-6C in detergent micelles. The reconstitution of AgrCTM5-6C or AgrCTM5-6C-GFP was characterized using dynamic light scattering, fluorescence microscopy, and transmission electron microscopy. Based on the results, the optimal conditions for protein incorporation were defined. These findings contribute to the study of membrane protein structure and function in vitro using a reconstitution system. PMID:24303011

15 CFR 290.5 - Basic proposal qualifications.

Code of Federal Regulations, 2011 CFR

2011-01-01

... INSTITUTE OF STANDARDS AND TECHNOLOGY, DEPARTMENT OF COMMERCE NIST EXTRAMURAL PROGRAMS REGIONAL CENTERS FOR THE TRANSFER OF MANUFACTURING TECHNOLOGY § 290.5 Basic proposal qualifications. (a) NIST shall... emphasis on areas consistent with NIST technology research programs and organizational expertise. (viii) A...

15 CFR 290.5 - Basic proposal qualifications.

Code of Federal Regulations, 2013 CFR

2013-01-01

... INSTITUTE OF STANDARDS AND TECHNOLOGY, DEPARTMENT OF COMMERCE NIST EXTRAMURAL PROGRAMS REGIONAL CENTERS FOR THE TRANSFER OF MANUFACTURING TECHNOLOGY § 290.5 Basic proposal qualifications. (a) NIST shall... emphasis on areas consistent with NIST technology research programs and organizational expertise. (viii) A...

15 CFR 290.5 - Basic proposal qualifications.

Code of Federal Regulations, 2014 CFR

2014-01-01

... INSTITUTE OF STANDARDS AND TECHNOLOGY, DEPARTMENT OF COMMERCE NIST EXTRAMURAL PROGRAMS REGIONAL CENTERS FOR THE TRANSFER OF MANUFACTURING TECHNOLOGY § 290.5 Basic proposal qualifications. (a) NIST shall... emphasis on areas consistent with NIST technology research programs and organizational expertise. (viii) A...

15 CFR 290.5 - Basic proposal qualifications.

Code of Federal Regulations, 2012 CFR

2012-01-01

... INSTITUTE OF STANDARDS AND TECHNOLOGY, DEPARTMENT OF COMMERCE NIST EXTRAMURAL PROGRAMS REGIONAL CENTERS FOR THE TRANSFER OF MANUFACTURING TECHNOLOGY § 290.5 Basic proposal qualifications. (a) NIST shall... emphasis on areas consistent with NIST technology research programs and organizational expertise. (viii) A...

25 CFR 170.506 - What are the minimum qualifications for certified bridge inspectors?

Code of Federal Regulations, 2011 CFR

2011-04-01

... 25 Indians 1 2011-04-01 2011-04-01 false What are the minimum qualifications for certified bridge... Program Facilities Bridge Inspection § 170.506 What are the minimum qualifications for certified bridge inspectors? The person responsible for the bridge inspection team must meet the qualifications for bridge...

Opportunities for the LWR ATF materials development program to contribute to the LBE-cooled ADS materials qualification program

NASA Astrophysics Data System (ADS)

Gong, Xing; Li, Rui; Sun, Maozhou; Ren, Qisen; Liu, Tong; Short, Michael P.

2016-12-01

Accelerator-driven systems (ADS) are a promising approach for nuclear waste disposal. Nevertheless, the principal candidate materials proposed for ADS construction, such as the ferritic/martensitic steel, T91, and austenitic stainless steels, 316L and 15-15Ti, are not fully compatible with the liquid lead-bismuth eutectic (LBE) coolant. Under some operating conditions, liquid metal embrittlement (LME) or liquid metal corrosion (LMC) may occur in these steels when exposed to LBE. These environmentally-induced material degradation effects pose a threat to ADS reactor safety, as failure of the materials could initiate a severe accident, in which fission products are released into the coolant. Meanwhile, parallel efforts to develop accident-tolerant fuels (ATF) in light water reactors (LWRs) could provide both general materials design philosophies and specific material solutions to the ADS program. In this paper, the potential contributions of the ATF materials development program to the ADS materials qualification program are evaluated and discussed in terms of service conditions and materials performance requirements. Several specific areas where coordinated development may benefit both programs, including composite materials and selected coatings, are discussed.

24 CFR 291.515 - Purchaser qualifications.

Code of Federal Regulations, 2010 CFR

2010-04-01

... in § 291.530) at the time he/she submits a bid to purchase a home through the program and at the time of closing on the purchase of the home; (b) The person must certify to his/her good faith intention... Next Door Sales Program § 291.515 Purchaser qualifications. To qualify to purchase a home through the...

AP-102/104 Retrieval control system qualification test procedure

DOE Office of Scientific and Technical Information (OSTI.GOV)

RIECK, C.A.

1999-05-18

This Qualification Test Procedure documents the results of the qualification testing that was performed on the Project W-211, ''Initial Tank Retrieval Systems,'' retrieval control system (RCS) for tanks 241-AP-102 and 241-AP-104. The results confirm that the RCS has been programmed correctly and that the two related hardware enclosures have been assembled in accordance with the design documents.

Results from the interim salt disposition program macrobatch 10 tank 21H qualification samples

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peters, T. B.; Bannochie, C. J.

2017-02-23

Savannah River National Laboratory (SRNL) analyzed samples from Tank 21H in support of qualification of Macrobatch (Salt Batch) 10 for the Interim Salt Disposition Program (ISDP). This document reports characterization data on the samples of Tank 21H and fulfills the requirements of Deliverable 3 of the Technical Task Request (TTR). Further work will report the results of the Extraction-Scrub-Strip (ESS) testing (Task 5 of the TTR) using the Tank 21H material. Task 4 of the TTR (MST Strike) will not be completed for Salt Batch 10.

The Staphylococcus aureus Two-Component System AgrAC Displays Four Distinct Genomic Arrangements That Delineate Genomic Virulence Factor Signatures

PubMed Central

Choudhary, Kumari S.; Mih, Nathan; Monk, Jonathan; Kavvas, Erol; Yurkovich, James T.; Sakoulas, George; Palsson, Bernhard O.

2018-01-01

Two-component systems (TCSs) consist of a histidine kinase and a response regulator. Here, we evaluated the conservation of the AgrAC TCS among 149 completely sequenced Staphylococcus aureus strains. It is composed of four genes: agrBDCA. We found that: (i) AgrAC system (agr) was found in all but one of the 149 strains, (ii) the agr positive strains were further classified into four agr types based on AgrD protein sequences, (iii) the four agr types not only specified the chromosomal arrangement of the agr genes but also the sequence divergence of AgrC histidine kinase protein, which confers signal specificity, (iv) the sequence divergence was reflected in distinct structural properties especially in the transmembrane region and second extracellular binding domain, and (v) there was a strong correlation between the agr type and the virulence genomic profile of the organism. Taken together, these results demonstrate that bioinformatic analysis of the agr locus leads to a classification system that correlates with the presence of virulence factors and protein structural properties. PMID:29887846

Implementation plans for the Korean certified tumor registrar qualification system.

PubMed

Boo, Yoo-Kyung; Lim, Hyun-Sook; Won, Young-Joo

2014-01-01

Cancer registration data is used to understand the nation's cancer burden, and to provide significant baseline data for cancer control efforts, as well as, research on cancer incidence, mortality, survival, and prevalence. A system that approves, assesses, and manages the qualification of specialists, responsible for performing cancer registration, has not been developed in Korea. This study presents ways to implement a certification system designed for the qualification of tumor registrars in Korea. Requirements for implementing a certified tumor registrar qualification system were determined by reviewing the system for establishing qualifications in Korea and the American qualification system via the National Cancer Registrars Association (NCRA). Moreover, a survey was conducted on Korean medical records administrators, who had taken the U.S. Certified Tumor Registrar (CTR) examination, in order to review their opinions regarding these requirements. This study verified the feasibility of a qualification examination based on the opinions of CTR specialists by determining the following: items, and the associated ratings, of the qualifications necessary to register individuals as certified tumor registrars in a private qualification system; status of human resources required for the examination or training processes; plans regarding the organization needed for management, and operation of qualifications, examination standards, subject areas, examination methods, examination qualifications, or education and training programs. The implementation of a certified tumor registrar qualification system will lead to enhanced job competency for specialists and a qualitative improvement of cancer registration data. It will also reliably foster human resources that will lay the groundwork needed to establish scientific and reasonable national cancer management policies.

14 CFR 91.1089 - Qualifications: Check pilots (aircraft) and check pilots (simulator).

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Qualifications: Check pilots (aircraft) and check pilots (simulator). 91.1089 Section 91.1089 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... RULES Fractional Ownership Operations Program Management § 91.1089 Qualifications: Check pilots...

14 CFR 91.1089 - Qualifications: Check pilots (aircraft) and check pilots (simulator).

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Qualifications: Check pilots (aircraft) and check pilots (simulator). 91.1089 Section 91.1089 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... RULES Fractional Ownership Operations Program Management § 91.1089 Qualifications: Check pilots...

Ceramography of Irradiated tristructural isotropic (TRISO) Fuel from the AGR-2 Experiment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rice, Francine Joyce; Stempien, John Dennis

2016-09-01

Ceramography was performed on cross sections from four tristructural isotropic (TRISO) coated particle fuel compacts taken from the AGR-2 experiment, which was irradiated between June 2010 and October 2013 in the Advanced Test Reactor (ATR). The fuel compacts examined in this study contained TRISO-coated particles with either uranium oxide (UO2) kernels or uranium oxide/uranium carbide (UCO) kernels that were irradiated to final burnup values between 9.0 and 11.1% FIMA. These examinations are intended to explore kernel and coating morphology evolution during irradiation. This includes kernel porosity, swelling, and migration, and irradiation-induced coating fracture and separation. Variations in behavior within amore » specific cross section, which could be related to temperature or burnup gradients within the fuel compact, are also explored. The criteria for categorizing post-irradiation particle morphologies developed for AGR-1 ceramographic exams, was applied to the particles in the AGR-2 compacts particles examined. Results are compared with similar investigations performed as part of the earlier AGR-1 irradiation experiment. This paper presents the results of the AGR-2 examinations and discusses the key implications for fuel irradiation performance.« less

14 CFR 121.909 - Approval of Advanced Qualification Program.

Code of Federal Regulations, 2011 CFR

2011-01-01

....915. All AQP curriculums must be based on an instructional systems development methodology. This... curriculum under this subpart is made, through the FAA office responsible for approval of the certificate... criteria. Each AQP must have separate curriculums for indoctrination, qualification, and continuing...

14 CFR 121.913 - Qualification curriculum.

Code of Federal Regulations, 2014 CFR

2014-01-01

... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Advanced Qualification Program § 121.913... planned hours of training, evaluation, and supervised operating experience. (b) For crewmembers, aircraft... activities that are aircraft- and equipment-specific to qualify a person for a particular duty position on...

14 CFR 121.913 - Qualification curriculum.

Code of Federal Regulations, 2013 CFR

2013-01-01

... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Advanced Qualification Program § 121.913... planned hours of training, evaluation, and supervised operating experience. (b) For crewmembers, aircraft... activities that are aircraft- and equipment-specific to qualify a person for a particular duty position on...

14 CFR 121.913 - Qualification curriculum.

Code of Federal Regulations, 2012 CFR

2012-01-01

... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Advanced Qualification Program § 121.913... planned hours of training, evaluation, and supervised operating experience. (b) For crewmembers, aircraft... activities that are aircraft- and equipment-specific to qualify a person for a particular duty position on...

Staphylococcus intermedius produces a functional agr autoinducing peptide containing a cyclic lactone.

PubMed

Ji, Guangyong; Pei, Wuhong; Zhang, Linsheng; Qiu, Rongde; Lin, Jianqun; Benito, Yvonne; Lina, Gerard; Novick, Richard P

2005-05-01

The agr system is a global regulator of accessory functions in staphylococci, including genes encoding exoproteins involved in virulence. The agr locus contains a two-component signal transduction module that is activated by an autoinducing peptide (AIP) encoded within the agr locus and is conserved throughout the genus. The AIP has an unusual partially cyclic structure that is essential for function and that, in all but one case, involves an internal thiolactone bond between a conserved cysteine and the C-terminal carboxyl group. The exceptional case is a strain of Staphylococcus intermedius that has a serine in place of the conserved cysteine. We demonstrate here that the S. intermedius AIP is processed by the S. intermedius AgrB protein to generate a cyclic lactone, that it is an autoinducer as well as a cross-inhibitor, and that all of five other S. intermedius strains examined also produce serine-containing AIPs.

Open-Source RTOS Space Qualification: An RTEMS Case Study

NASA Technical Reports Server (NTRS)

Zemerick, Scott

2017-01-01

NASA space-qualification of reusable off-the-shelf real-time operating systems (RTOSs) remains elusive due to several factors notably (1) The diverse nature of RTOSs utilized across NASA, (2) No single NASA space-qualification criteria, lack of verification and validation (V&V) analysis, or test beds, and (3) different RTOS heritages, specifically open-source RTOSs and closed vendor-provided RTOSs. As a leader in simulation test beds, the NASA IV&V Program is poised to help jump-start and lead the space-qualification effort of the open source Real-Time Executive for Multiprocessor Systems (RTEMS) RTOS. RTEMS, as a case-study, can be utilized as an example of how to qualify all RTOSs, particularly the reusable non-commercial (open-source) ones that are gaining usage and popularity across NASA. Qualification will improve the overall safety and mission assurance of RTOSs for NASA-agency wide usage. NASA's involvement in space-qualification of an open-source RTOS such as RTEMS will drive the RTOS industry toward a more qualified and mature open-source RTOS product.

42 CFR 410.134 - Provider qualifications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 2 2012-10-01 2012-10-01 false Provider qualifications. 410.134 Section 410.134 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES MEDICARE PROGRAM SUPPLEMENTARY MEDICAL INSURANCE (SMI) BENEFITS Medical Nutrition Therapy § 410.134 Provider...

42 CFR 410.134 - Provider qualifications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 2 2011-10-01 2011-10-01 false Provider qualifications. 410.134 Section 410.134 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES MEDICARE PROGRAM SUPPLEMENTARY MEDICAL INSURANCE (SMI) BENEFITS Medical Nutrition Therapy § 410.134 Provider...

42 CFR 410.134 - Provider qualifications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 2 2010-10-01 2010-10-01 false Provider qualifications. 410.134 Section 410.134 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES MEDICARE PROGRAM SUPPLEMENTARY MEDICAL INSURANCE (SMI) BENEFITS Medical Nutrition Therapy § 410.134 Provider...

42 CFR 410.134 - Provider qualifications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 2 2014-10-01 2014-10-01 false Provider qualifications. 410.134 Section 410.134 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES MEDICARE PROGRAM SUPPLEMENTARY MEDICAL INSURANCE (SMI) BENEFITS Medical Nutrition Therapy § 410.134 Provider...

42 CFR 410.134 - Provider qualifications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 2 2013-10-01 2013-10-01 false Provider qualifications. 410.134 Section 410.134 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES MEDICARE PROGRAM SUPPLEMENTARY MEDICAL INSURANCE (SMI) BENEFITS Medical Nutrition Therapy § 410.134 Provider...

Faculty Qualification Policies and Strategies Relevant to Dual Enrollment Programs: An Analysis of States and Regional Accreditation Agencies. Policy Report

ERIC Educational Resources Information Center

Horn, Aaron S.; Reinert, Leah; Jang, Sung Tae; Zinth, Jennifer Dounay

2016-01-01

As dual enrollment programs continue to expand, a critical challenge is to ensure that the quality of such courses offered in high schools is equivalent to the quality of courses taught in postsecondary institutions. Standards for faculty qualifications have historically constituted one facet of efforts to regulate educational quality, and thus…

Cross-Talk between Staphylococcus aureus and Other Staphylococcal Species via the agr Quorum Sensing System

PubMed Central

Canovas, Jaime; Baldry, Mara; Bojer, Martin S.; Andersen, Paal S.; Gless, Bengt H.; Grzeskowiak, Piotr K.; Stegger, Marc; Damborg, Peter; Olsen, Christian A.; Ingmer, Hanne

2016-01-01

Staphylococci are associated with both humans and animals. While most are non-pathogenic colonizers, Staphylococcus aureus is an opportunistic pathogen capable of causing severe infections. S. aureus virulence is controlled by the agr quorum sensing system responding to secreted auto-inducing peptides (AIPs) sensed by AgrC, a two component histidine kinase. agr loci are found also in other staphylococcal species and for Staphylococcus epidermidis, the encoded AIP represses expression of agr regulated virulence genes in S. aureus. In this study we aimed to better understand the interaction between staphylococci and S. aureus, and show that this interaction may eventually lead to the identification of new anti-virulence candidates to target S. aureus infections. Here we show that culture supernatants of 37 out of 52 staphylococcal isolates representing 17 different species inhibit S. aureus agr. The dog pathogen, Staphylococcus schleiferi, expressed the most potent inhibitory activity and was active against all four agr classes found in S. aureus. By employing a S. aureus strain encoding a constitutively active AIP receptor we show that the activity is mediated via agr. Subsequent cloning and heterologous expression of the S. schleiferi AIP in S. aureus demonstrated that this molecule was likely responsible for the inhibitory activity, and further proof was provided when pure synthetic S. schleiferi AIP was able to completely abolish agr induction of an S. aureus reporter strain. To assess impact on S. aureus virulence, we co-inoculated S. aureus and S. schleiferi in vivo in the Galleria mellonella wax moth larva, and found that expression of key S. aureus virulence factors was abrogated. Our data show that the S. aureus agr locus is highly responsive to other staphylococcal species suggesting that agr is an inter-species communication system. Based on these results we speculate that interactions between S. aureus and other colonizing staphylococci will significantly

Building a roadmap to biomarker qualification: challenges and opportunities.

PubMed

Amur, Shashi G; Sanyal, Sarmistha; Chakravarty, Aloka G; Noone, Marianne H; Kaiser, James; McCune, Susan; Buckman-Garner, ShaAvhree Y

2015-01-01

The traditional route for regulatory acceptance of biomarkers in drug development is through submission of biomarker data in drug approval submissions in the context of a single drug development program. The US FDA's Critical Path Initiative called for establishment of a biomarker qualification process to enable progress in the drug development paradigm. In response to this, the Center for Drug Evaluation and Research (CDER) established a Biomarker Qualification Program (BQP) to qualify a biomarker for a specific context of use (COU). The qualified biomarker can then be used in multiple drug development programs for this COU without re-review. Here, we describe some of the features of the BQP and two new initiatives that have the potential to aid biomarker development through early interactions with the FDA. Finally, we discuss some of the feedback the FDA has received from submitters and the BQP's actions to strengthen the program.

Tamoxifen-Dependent Induction of AGR2 Is Associated with Increased Aggressiveness of Endometrial Cancer Cells.

PubMed

Hrstka, Roman; Podhorec, Jan; Nenutil, Rudolf; Sommerova, Lucia; Obacz, Joanna; Durech, Michal; Faktor, Jakub; Bouchal, Pavel; Skoupilova, Hana; Vojtesek, Borivoj

2017-05-28

Tamoxifen treatment in breast cancer patients is associated with increased risk of endometrial malignancies. Significantly, higher AGR2 expression was found in endometrial cancers that developed in women previously treated with tamoxifen compared to those who had not been exposed to tamoxifen. An association of elevated AGR2 level with myometrial invasion occurrence and invasion depth was also found. In vitro analyses identified a stimulatory effect of AGR2 on cellular proliferation. Although adverse tamoxifen effects on endometrial cells remain elusive, our work identifies elevated AGR2 as a candidate tamoxifen-dependent mechanism of action responsible for increased incidence of endometrial cancer.

DOE handbook: Guide to good practices for training and qualification of maintenance personnel

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1996-03-01

The purpose of this Handbook is to provide contractor training organizations with information that can be used to verify the adequacy of and/or modify existing maintenance training programs, or to develop new training programs. This guide, used in conjunction with facility-specific job analyses, provides a framework for training and qualification programs for maintenance personnel at DOE reactor and nonreactor nuclear facilities. Recommendations for qualification are made in four areas: education, experience, physical attributes, and training. The functional positions of maintenance mechanic, electrician, and instrumentation and control technician are covered by this guide. Sufficient common knowledge and skills were found tomore » include the three disciplines in one guide to good practices. Contents include: qualifications; on-the-job training; trainee evaluation; continuing training; training effectiveness evaluation; and program records. Appendices are included which relate to: administrative training; industrial safety training; fundamentals training; tools and equipment training; facility systems and component knowledge training; facility systems and component skills training; and specialized skills training.« less

The Effect of Birthrate Granularity on the Release- to- Birth Ratio for the AGR-1 In-core Experiment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dawn Scates; John Walter

The AGR-1 Advanced Gas Reactor (AGR) tristructural-isotropic-particle fuel experiment underwent 13 irradiation intervals from December 2006 until November 2009 within the Idaho National Laboratory Advanced Test Reactor in support of the Next Generation Nuclear Power Plant program. During this multi-year experiment, release-to-birth rate ratios were computed at the end of each operating interval to provide information about fuel performance. Fission products released during irradiation were tracked daily by the Fission Product Monitoring System using 8-hour measurements. Birth rates calculated by MCNP with ORIGEN for as-run conditions were computed at the end of each irradiation interval. Each time step in MCNPmore » provided neutron flux, reaction rates and AGR-1 compact composition, which were used to determine birth rates using ORIGEN. The initial birth-rate data, consisting of four values for each irradiation interval at the beginning, end, and two intermediate times, were interpolated to obtain values for each 8-hour activity. The problem with this method is that any daily changes in heat rates or perturbations, such as shim control movement or core/lobe power fluctuations, would not be reflected in the interpolated data and a true picture of the system would not be presented. At the conclusion of the AGR-1 experiment, great efforts were put forth to compute daily birthrates, which were reprocessed with the 8-hour release activity. The results of this study are presented in this paper.« less

The effect of birthrate granularity on the release-to-birth ratio for the AGR-1 in-core experiment

DOE Office of Scientific and Technical Information (OSTI.GOV)

D. M. Scates; J. B. Walter; J. T. Maki

The AGR-1 Advanced Gas Reactor (AGR) tristructural-isotropic-particle fuel experiment underwent 13 irradiation intervals from December 2006 until November 2009 within the Idaho National Laboratory Advanced Test Reactor in support of the Next Generation Nuclear Power Plant program. During this multi-year experiment, release-to-birth rate ratios were computed at the end of each operating interval to provide information about fuel performance. Fission products released during irradiation were tracked daily by the Fission Product Monitoring System using 8-h measurements. Birth rate calculated by MCNP with ORIGEN for as-run conditions were computed at the end of each irradiation interval. Each time step in MCNPmore » provided neutron flux, reaction rates and AGR-1 compact composition, which were used to determine birth rate using ORIGEN. The initial birth-rate data, consisting of four values for each irradiation interval at the beginning, end, and two intermediate times, were interpolated to obtain values for each 8-h activity. The problem with this method is that any daily changes in heat rates or perturbations, such as shim control movement or core/lobe power fluctuations, would not be reflected in the interpolated data and a true picture of the system would not be presented. At the conclusion of the AGR-1 experiment, great efforts were put forth to compute daily birthrates, which were reprocessed with the 8-h release activity. The results of this study are presented in this paper.« less

Selective Chemical Inhibition of agr Quorum Sensing in Staphylococcus aureus Promotes Host Defense with Minimal Impact on Resistance

PubMed Central

Sully, Erin K.; Malachowa, Natalia; Elmore, Bradley O.; Alexander, Susan M.; Femling, Jon K.; Gray, Brian M.; DeLeo, Frank R.; Otto, Michael; Cheung, Ambrose L.; Edwards, Bruce S.; Sklar, Larry A.; Horswill, Alexander R.; Hall, Pamela R.; Gresham, Hattie D.

2014-01-01

Bacterial signaling systems are prime drug targets for combating the global health threat of antibiotic resistant bacterial infections including those caused by Staphylococcus aureus. S. aureus is the primary cause of acute bacterial skin and soft tissue infections (SSTIs) and the quorum sensing operon agr is causally associated with these. Whether efficacious chemical inhibitors of agr signaling can be developed that promote host defense against SSTIs while sparing the normal microbiota of the skin is unknown. In a high throughput screen, we identified a small molecule inhibitor (SMI), savirin (S. aureus virulence inhibitor) that disrupted agr-mediated quorum sensing in this pathogen but not in the important skin commensal Staphylococcus epidermidis. Mechanistic studies employing electrophoretic mobility shift assays and a novel AgrA activation reporter strain revealed the transcriptional regulator AgrA as the target of inhibition within the pathogen, preventing virulence gene upregulation. Consistent with its minimal impact on exponential phase growth, including skin microbiota members, savirin did not provoke stress responses or membrane dysfunction induced by conventional antibiotics as determined by transcriptional profiling and membrane potential and integrity studies. Importantly, savirin was efficacious in two murine skin infection models, abating tissue injury and selectively promoting clearance of agr+ but not Δagr bacteria when administered at the time of infection or delayed until maximal abscess development. The mechanism of enhanced host defense involved in part enhanced intracellular killing of agr+ but not Δagr in macrophages and by low pH. Notably, resistance or tolerance to savirin inhibition of agr was not observed after multiple passages either in vivo or in vitro where under the same conditions resistance to growth inhibition was induced after passage with conventional antibiotics. Therefore, chemical inhibitors can selectively target AgrA in

agr-Dependent Interactions of Staphylococcus aureus USA300 with Human Polymorphonuclear Neutrophils

PubMed Central

Pang, Yun Yun; Schwartz, Jamie; Thoendel, Matthew; Ackermann, Laynez W.; Horswill, Alexander R.; Nauseef, William M.

2010-01-01

The emergence of serious infections due to community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has fueled interest in the contributions of specific staphylococcal virulence factors to clinical disease. To assess the contributions of agr-dependent factors to the fate of organisms in polymorphonuclear neutrophils (PMN), we examined the consequences for organism and host cells of feeding PMN with wild-type CA-MRSA (LAC) or CA-MRSA (LAC agr KO) at different multiplicities of infection (MOIs). Phagocytosed organisms rapidly increased the transcription of RNAIII in a time- and MOI-dependent fashion; extracellular USA300 (LAC) did not increase RNAIII expression despite having the capacity to respond to autoinducing peptide-enriched culture medium. HOCl-mediated damage and intracellular survival were the same in the wild-type and USA300 (LAC agr KO). PMN lysis by ingested USA300 (LAC) was time- and MOI-dependent and, at MOIs >1, required α-hemolysin (hla) as USA300 (LAC agr KO) and USA300 (LAC hla KO) promoted PMN lysis only at high MOIs. Taken together, these data demonstrate activation of the agr operon in human PMN with the subsequent production of α-hemolysin and PMN lysis. The extent to which these events in the phagosomes of human PMN contribute to the increased morbidity and mortality of infections with USA300 (LAC) merits further study. PMID:20829608

The Agr communication system provides a benefit to the populations of Listeria monocytogenes in soil.

PubMed

Vivant, Anne-Laure; Garmyn, Dominique; Gal, Laurent; Piveteau, Pascal

2014-01-01

In this study, we investigated whether the Agr communication system of the pathogenic bacterium Listeria monocytogenes was involved in adaptation and competitiveness in soil. Alteration of the ability to communicate, either by deletion of the gene coding the response regulator AgrA (response-negative mutant) or the signal pro-peptide AgrD (signal-negative mutant), did not affect population dynamics in soil that had been sterilized but survival was altered in biotic soil suggesting that the Agr system of L. monocytogenes was involved to face the complex soil biotic environment. This was confirmed by a set of co-incubation experiments. The fitness of the response-negative mutant was lower either in the presence or absence of the parental strain but the fitness of the signal-negative mutant depended on the strain with which it was co-incubated. The survival of the signal-negative mutant was higher when co-cultured with the parental strain than when co-cultured with the response-negative mutant. These results showed that the ability to respond to Agr communication provided a benefit to listerial cells to compete. These results might also indicate that in soil, the Agr system controls private goods rather than public goods.

15 CFR 290.5 - Basic proposal qualifications.

Code of Federal Regulations, 2010 CFR

2010-01-01

... INSTITUTE OF STANDARDS AND TECHNOLOGY, DEPARTMENT OF COMMERCE NIST EXTRAMURAL PROGRAMS REGIONAL CENTERS FOR THE TRANSFER OF MANUFACTURING TECHNOLOGY § 290.5 Basic proposal qualifications. (a) NIST shall... with any invention or copyright which may result from the involvement in the Center's technology...

Anterior Gradient 2 (AGR2) Induced Epidermal Growth Factor Receptor (EGFR) Signaling Is Essential for Murine Pancreatitis-Associated Tissue Regeneration

PubMed Central

Wodziak, Dariusz; Dong, Aiwen; Basin, Michael F.; Lowe, Anson W.

2016-01-01

A recently published study identified Anterior Gradient 2 (AGR2) as a regulator of EGFR signaling by promoting receptor presentation from the endoplasmic reticulum to the cell surface. AGR2 also promotes tissue regeneration in amphibians and fish. Whether AGR2-induced EGFR signaling is essential for tissue regeneration in higher vertebrates was evaluated using a well-characterized murine model for pancreatitis. The impact of AGR2 expression and EGFR signaling on tissue regeneration was evaluated using the caerulein-induced pancreatitis mouse model. EGFR signaling and cell proliferation were examined in the context of the AGR2-/- null mouse or with the EGFR-specific tyrosine kinase inhibitor, AG1478. In addition, the Hippo signaling coactivator YAP1 was evaluated in the context of AGR2 expression during pancreatitis. Pancreatitis-induced AGR2 expression enabled EGFR translocation to the plasma membrane, the initiation of cell signaling, and cell proliferation. EGFR signaling and tissue regeneration were partially inhibited by the tyrosine kinase inhibitor AG1478, but absent in the AGR2-/- null mouse. AG1478-treated and AGR2-/- null mice with pancreatitis died whereas all wild-type controls recovered. YAP1 activation was also dependent on pancreatitis-induced AGR2 expression. AGR2-induced EGFR signaling was essential for tissue regeneration and recovery from pancreatitis. The results establish tissue regeneration as a major function of AGR2-induced EGFR signaling in adult higher vertebrates. Enhanced AGR2 expression and EGFR signaling are also universally present in human pancreatic cancer, which support a linkage between tissue injury, regeneration, and cancer pathogenesis. PMID:27764193

29 CFR 1960.25 - Qualifications of safety and health inspectors and agency inspections.

Code of Federal Regulations, 2012 CFR

2012-07-01

... environments where there are less complex hazards, such safety and health specializations as cited above may... 29 Labor 9 2012-07-01 2012-07-01 false Qualifications of safety and health inspectors and agency... OCCUPATIONAL SAFETY AND HEALTH PROGRAMS AND RELATED MATTERS Inspection and Abatement § 1960.25 Qualifications...

29 CFR 1960.25 - Qualifications of safety and health inspectors and agency inspections.

Code of Federal Regulations, 2010 CFR

2010-07-01

... environments where there are less complex hazards, such safety and health specializations as cited above may... 29 Labor 9 2010-07-01 2010-07-01 false Qualifications of safety and health inspectors and agency... OCCUPATIONAL SAFETY AND HEALTH PROGRAMS AND RELATED MATTERS Inspection and Abatement § 1960.25 Qualifications...

29 CFR 1960.25 - Qualifications of safety and health inspectors and agency inspections.

Code of Federal Regulations, 2014 CFR

2014-07-01

... environments where there are less complex hazards, such safety and health specializations as cited above may... 29 Labor 9 2014-07-01 2014-07-01 false Qualifications of safety and health inspectors and agency... OCCUPATIONAL SAFETY AND HEALTH PROGRAMS AND RELATED MATTERS Inspection and Abatement § 1960.25 Qualifications...

29 CFR 1960.25 - Qualifications of safety and health inspectors and agency inspections.

Code of Federal Regulations, 2011 CFR

2011-07-01

... environments where there are less complex hazards, such safety and health specializations as cited above may... 29 Labor 9 2011-07-01 2011-07-01 false Qualifications of safety and health inspectors and agency... OCCUPATIONAL SAFETY AND HEALTH PROGRAMS AND RELATED MATTERS Inspection and Abatement § 1960.25 Qualifications...

29 CFR 1960.25 - Qualifications of safety and health inspectors and agency inspections.

Code of Federal Regulations, 2013 CFR

2013-07-01

... environments where there are less complex hazards, such safety and health specializations as cited above may... 29 Labor 9 2013-07-01 2013-07-01 false Qualifications of safety and health inspectors and agency... OCCUPATIONAL SAFETY AND HEALTH PROGRAMS AND RELATED MATTERS Inspection and Abatement § 1960.25 Qualifications...

[Equipment qualification].

PubMed

Le Vacon, F

2005-06-01

The qualification of the equipment is a particularly important stage in the transfusional process. On the one hand, of many standards such as those of certification or that of accreditation require it, just as the good transfusional practices; in addition, the practices of steps of quality assurance develop this aspect. Indeed, the absence of the realization of this qualification of material having an influence on the finished product, can lead to an error in the product. This qualification passes by various stages of which some are major such as the drafting of the schedule of conditions, the drafting of the operational protocol of qualification, the decision made for the setting in routine. Finally so that this qualification takes all its dimensions it is necessary to carry out methods linked to the international system of measurement. Moreover certain questions after reflexions must find response such as which unit to check, and only this one, the equipment is - it a complex one, is there a maintenance contract? Once all these elements taken into account, the questions having found their answer, the operational protocol will then well be built, the decisions of settings in routine could be done and the sets of the finalized stages.

CD147 and AGR2 expression promote cellular proliferation and metastasis of head and neck squamous cell carcinoma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sweeny, Larissa, E-mail: larissasweeny@gmail.com; Liu, Zhiyong; Bush, Benjamin D.

2012-08-15

The signaling pathways facilitating metastasis of head and neck squamous cell carcinoma (HNSCC) cells are not fully understood. CD147 is a transmembrane glycoprotein known to induce cell migration and invasion. AGR2 is a secreted peptide also known to promote cell metastasis. Here we describe their importance in the migration and invasion of HNSCC cells (FADU and OSC-19) in vitro and in vivo. In vitro, knockdown of CD147 or AGR2 decreased cellular proliferation, migration and invasion. In vivo, knockdown of CD147 or AGR2 expression decreased primary tumor growth as well as regional and distant metastasis. -- Highlights: Black-Right-Pointing-Pointer We investigated AGR2more » in head and neck squamous cell carcinoma for the first time. Black-Right-Pointing-Pointer We explored the relationship between AGR2 and CD147 for the first time. Black-Right-Pointing-Pointer AGR2 and CD147 appear to co-localize in head and squamous cell carcinoma samples. Black-Right-Pointing-Pointer Knockdown of both AGR2 and CD147 reduced migration and invasion in vitro. Black-Right-Pointing-Pointer Knockdown of both AGR2 and CD147 decreased metastasis in vivo.« less

[Additional qualification in health economics--a pre-condition for ENT leadership positions?].

PubMed

Lehnerdt, G; Schöffski, O; Mattheis, S; Hoffmann, T K; Lang, S

2013-11-01

The increasing medical-technical progress as well as the dramatic demographic changes cause problems with regard to rapid enlargement of medical service offers, allocation of resources and a financing shortfall in the German public health system. The economization in the German Health System can also be perceived in ENT departments. After performing an internet search about the rapidly growing market for qualifications measures in health economics, we hence conducted an anonymous survey for ENT senior doctors and directors of the 34 German University Departments to evaluate their attitude towards, as well as their expectation of such an add-on qualification. Since the German government finalized the health care reform in the year 2000 such qualification measures rapidly developed: amongst others, 26 postgraduate, extra-occupational master programs have been inaugurated. The anonymous survey was answered by 105 ENT doctors (63 senior doctors, 27 vice professors and 15 directors). 63% out of these 105 colleagues considered such an add-on qualification to be mandatory. 41% of the colleagues were already "add-on qualified" in that field, only 10 of them by means of a study program. 71 of 105 colleagues (68%) considered the add-on qualification to be advantageous for their future personal career. With regard to the designated contents of the study program, "Staff Management" was even prioritized to "Hospital Financing" and "Cost Accounting". Aspects of management and a (health-) economical basic knowledge became an integral part of the daily routine for "first-line management doctors" also in (University) ENT-departments. © Georg Thieme Verlag KG Stuttgart · New York.

Conservation and Evolutionary Dynamics of the agr Cell-to-Cell Communication System across Firmicutes▿ †

PubMed Central

Wuster, Arthur; Babu, M. Madan

2008-01-01

We present evidence that the agr cell-to-cell communication system is present across firmicutes, including the human pathogen Clostridium perfringens. Although we find that the agr system is evolutionarily conserved and that the general functions which it regulates are similar in different species, the individual regulated genes are not the same. This suggests that the regulatory network controlled by agr is dynamic and evolves rapidly. PMID:17933897

Australian Qualifications Framework Lower-Level Qualifications: Pathways to Where for Young People?

ERIC Educational Resources Information Center

Stanwick, John

2005-01-01

This study investigates where certificate I and II qualifications lead young people aged 15-24 years in terms of employment and further study. A prime motivation for young people undertaking these qualifications is to facilitate transition into the labour market. These qualifications are aimed at developing basic vocational skills or preparatory…

The Agr communication system provides a benefit to the populations of Listeria monocytogenes in soil

PubMed Central

Vivant, Anne-Laure; Garmyn, Dominique; Gal, Laurent; Piveteau, Pascal

2014-01-01

In this study, we investigated whether the Agr communication system of the pathogenic bacterium Listeria monocytogenes was involved in adaptation and competitiveness in soil. Alteration of the ability to communicate, either by deletion of the gene coding the response regulator AgrA (response-negative mutant) or the signal pro-peptide AgrD (signal-negative mutant), did not affect population dynamics in soil that had been sterilized but survival was altered in biotic soil suggesting that the Agr system of L. monocytogenes was involved to face the complex soil biotic environment. This was confirmed by a set of co-incubation experiments. The fitness of the response-negative mutant was lower either in the presence or absence of the parental strain but the fitness of the signal-negative mutant depended on the strain with which it was co-incubated. The survival of the signal-negative mutant was higher when co-cultured with the parental strain than when co-cultured with the response-negative mutant. These results showed that the ability to respond to Agr communication provided a benefit to listerial cells to compete. These results might also indicate that in soil, the Agr system controls private goods rather than public goods. PMID:25414837

AZ-101 Mixer Pump Test Qualification Test Procedures (QTP)

DOE Office of Scientific and Technical Information (OSTI.GOV)

THOMAS, W.K.

2000-01-10

Describes the Qualification test procedure for the AZ-101 Mixer Pump Data Acquisition System (DAS). The purpose of this Qualification Test Procedure (QTP) is to confirm that the AZ-101 Mixer Pump System has been properly programmed and hardware configured correctly. This QTP will test the software setpoints for the alarms and also check the wiring configuration from the SIMcart to the HMI. An Acceptance Test Procedure (ATP), similar to this QTP will be performed to test field devices and connections from the field.

Sub-inhibitory concentrations of oxacillin modify the expression of agr locus in Staphylococcus aureus clinical strains belonging to different clonal complexes.

PubMed

Viedma, Esther; Pérez-Montarelo, Dafne; Villa, Jennifer; Muñoz-Gallego, Irene; Larrosa, Nieves; Fernández-Hidalgo, Nuria; Gavaldà, Joan; Almirante, Benito; Chaves, Fernando

2018-04-16

The ability of Staphylococcus aureus to invade tissues and cause an infectious disease is the result of a multi-factorial process supported by the huge number of virulence factors inherent to this microorganism tightly regulated by the accessory gene regulator (agr). During antimicrobial therapy bacteria may be exposed to sub-inhibitory concentrations (subMICs) of antibiotics that may trigger transcriptional changes that may have an impact on the pathogenesis of infection. The objective of this study was to investigate the effect of oxacillin sub-MICs on agr system expression as the key component in the regulation of virulence in methicillin-susceptible (MSSA) and -resistant S. aureus (MRSA) strains. Furthermore, we studied the genetic basis of the agr locus and their potential association with the expression levels. We have examined the expression of RNAIII and agrA mRNA as biomarkers for agr expression in the presence and absence of oxacillin subMICs in 10 MSSA and 4 MRSA clinical strains belonging to 5 clonal complexes (CC45-agrI, CC8-agrI, CC5-agrII, CC15-agrII and CC30-agrIII) causing endovascular complications. The DNA sequences of agr locus were obtained by whole genome sequencing. Our results revealed that exposure to subMICs of oxacillin had an impact on agr locus expression modifying the relative levels of expression with increases in 11 strains and with decreases in 3 strains. Thereby, the exposure to subMICs of oxacillin resulted in higher levels of expression of agr in CC15 and CC45 and lower levels in CC30. We also observed the presence of mutations in agrC and agrA in 13/14 strains with similar mutation profiles among strains within individual CCs except for strains of CC5. Although, agr expression levels differed among strains within CCs, the presence of these mutations was associated with differences in agr expression levels in most cases. Changes in agr expression induced by exposure to oxacillin subMICs should be considered because they could

15 CFR 295.31 - Qualification of proposers.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Qualification of proposers. 295.31 Section 295.31 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY, DEPARTMENT OF COMMERCE NIST EXTRAMURAL PROGRAMS ADVANCED TECHNOLOGY...

15 CFR 295.31 - Qualification of proposers.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Qualification of proposers. 295.31 Section 295.31 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY, DEPARTMENT OF COMMERCE NIST EXTRAMURAL PROGRAMS ADVANCED TECHNOLOGY...

15 CFR 295.31 - Qualification of proposers.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Qualification of proposers. 295.31 Section 295.31 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY, DEPARTMENT OF COMMERCE NIST EXTRAMURAL PROGRAMS ADVANCED TECHNOLOGY...

15 CFR 295.31 - Qualification of proposers.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Qualification of proposers. 295.31 Section 295.31 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY, DEPARTMENT OF COMMERCE NIST EXTRAMURAL PROGRAMS ADVANCED TECHNOLOGY...

15 CFR 295.31 - Qualification of proposers.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Qualification of proposers. 295.31 Section 295.31 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY, DEPARTMENT OF COMMERCE NIST EXTRAMURAL PROGRAMS ADVANCED TECHNOLOGY...

The Agr-Like Quorum Sensing System Is Required for Pathogenesis of Necrotic Enteritis Caused by Clostridium perfringens in Poultry.

PubMed

Yu, Qiang; Lepp, Dion; Mehdizadeh Gohari, Iman; Wu, Tao; Zhou, Hongzhuan; Yin, Xianhua; Yu, Hai; Prescott, John F; Nie, Shao-Ping; Xie, Ming-Yong; Gong, Joshua

2017-06-01

Clostridium perfringens encodes at least two different quorum sensing (QS) systems, the Agr-like and LuxS, and recent studies have highlighted their importance in the regulation of toxin production and virulence. The role of QS in the pathogenesis of necrotic enteritis (NE) in poultry and the regulation of NetB, the key toxin involved, has not yet been investigated. We have generated isogenic agrB -null and complemented strains from parent strain CP1 and demonstrated that the virulence of the agrB -null mutant was strongly attenuated in a chicken NE model system and restored by complementation. The production of NetB, a key NE-associated toxin, was dramatically reduced in the agrB mutant at both the transcriptional and protein levels, though not in a luxS mutant. Transwell assays confirmed that the Agr-like QS system controls NetB production through a diffusible signal. Global gene expression analysis of the agrB mutant identified additional genes modulated by Agr-like QS, including operons related to phospholipid metabolism and adherence, which may also play a role in NE pathogenesis. This study provides the first evidence that the Agr-like QS system is critical for NE pathogenesis and identifies a number of Agr-regulated genes, most notably netB , that are potentially involved in mediating its effects. The Agr-like QS system thus may serve as a target for developing novel interventions to prevent NE in chickens. © Crown copyright 2017.

Estándar para la Protección del Trabajador Agrícola Revisado

EPA Pesticide Factsheets

Estas revisiones al Estándar de Protección a los Trabajadores Agrícolas, promulgado en 1992, proporcionarán protecciones de salud a los trabajadores agrícolas similares a las que ya disponen trabajadores en otras industrias.

Development and qualification testing of a laser-ignited, all-secondary (DDT) detonator

NASA Technical Reports Server (NTRS)

Blachowski, Thomas J.; Krivitsky, Darrin Z.; Tipton, Stephen

1994-01-01

The Indian Head Division, Naval Surface Warfare Center (IHDIV, NSWC) is conducting a qualification program for a laser-ignited, all-secondary (DDT) explosive detonator. This detonator was developed jointly by IHDIV, NSWC and the Department of Energy's EG&G Mound Applied Technologies facility in Miamisburg, Ohio to accept a laser initiation signal and produce a fully developed shock wave output. The detonator performance requirements were established by the on-going IHDIV, NSWC Laser Initiated Transfer Energy Subsystem (LITES) advanced development program. Qualification of the detonator as a component utilizing existing military specifications is the selected approach for this program. The detonator is a deflagration-to-detonator transfer (DDT) device using a secondary explosive, HMX, to generate the required shock wave output. The prototype development and initial system integration tests for the LITES and for the detonator were reported at the 1992 International Pyrotechnics Society Symposium and at the 1992 Survival and Flight Equipment National Symposium. Recent results are presented for the all-fire sensitivity and qualification tests conducted at two different laser initiation pulses.

7 CFR 4280.109 - Qualification for simplified applications.

Code of Federal Regulations, 2010 CFR

2010-01-01

...-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE LOANS AND GRANTS Renewable Energy Systems and Energy Efficiency Improvements Program Section A. Grants § 4280.109 Qualification for... must use commercially available renewable energy systems or energy efficiency improvements. (5...

7 CFR 4280.109 - Qualification for simplified applications.

Code of Federal Regulations, 2011 CFR

2011-01-01

...-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE LOANS AND GRANTS Renewable Energy Systems and Energy Efficiency Improvements Program Section A. Grants § 4280.109 Qualification for... must use commercially available renewable energy systems or energy efficiency improvements. (5...

BSM Delta Qualification 2, volume 3, book 2

NASA Technical Reports Server (NTRS)

1994-01-01

This report, presented in three volumes, provides the results of a two-motor Delta Qualification 2 program conducted in 1993 to certify the following enhancements for incorporation into booster separation motor (BSM0 flight hardware: vulcanized-in-place nozzle aft closure insulation; new iso-static ATJ bulk graphite throat insert material, adhesive EA9394 for bonding the nozzle throat, igniter grain rod/centering insert/igniter case; deletion of the igniter adapter insulator ring; deletion of the igniter adapter/igniter case interface RTV; and deletion of loctite from igniter retainer plate threads. The enhancements above directly resulted from (1) the BSM total quality management (TQM) team initiatives to enhance the BSM producibility, and (2) the necessity to qualify new throat insert and adhesive systems to replace existing materials that will not be available. Testing was completed at both the component and motor levels. Component testing was accomplished to screen candidate materials (e.g., throat materials, adhesive systems) and to optimize processes (e.g., aft closure insulator vulcanization approach) prior to their incorporation into the test motors. Motor testing--consisting of two motors, randomly selected by USBI's on-site quality personnel from production lot AAY, which were modified to accept the enhancements -- was completed to provide the final qualification of the enhancements for incorporation into flight hardware. Volume 3, Book 2 provides various supporting documentation to the previous volumes with regards to the testing of the two Delta qualification units: data acceptance records, thermal conditioning analysis, igniter adapter thermal flake analysis, laboratory adhesive (EA-9394) qualification report, throat insert thermal/structural analysis, Delta Qualification Nonconformance Reports (NCR's), O-ring seating tests, and interim test report for vulcanization process qualification.

Acceptance Test Data for Candidate AGR-5/6/7 TRISO Particle Batches BWXT Coater Batches 93165 93172 Defective IPyC Fraction and Pyrocarbon Anisotropy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Helmreich, Grant W.; Hunn, John D.; Skitt, Darren J.

2017-03-01

Coated particle fuel batches J52O-16-93165, 93166, 93168, 93169, 93170, and 93172 were produced by Babcock and Wilcox Technologies (BWXT) for possible selection as fuel for the Advanced Gas Reactor Fuel Development and Qualification (AGR) Program’s AGR-5/6/7 irradiation test in the Idaho National Laboratory (INL) Advanced Test Reactor (ATR). Some of these batches may alternately be used as demonstration coated particle fuel for other experiments. Each batch was coated in a 150-mm-diameter production-scale fluidized-bed chemical vapor deposition (CVD) furnace. Tristructural isotropic (TRISO) coatings were deposited on 425-μm-nominal-diameter spherical kernels from BWXT lot J52R-16-69317 containing a mixture of 15.5%-enriched uranium carbide andmore » uranium oxide (UCO). The TRISO coatings consisted of four consecutive CVD layers: a ~50% dense carbon buffer layer with 100-μm-nominal thickness, a dense inner pyrolytic carbon (IPyC) layer with 40-μm-nominal thickness, a silicon carbide (SiC) layer with 35-μm-nominal thickness, and a dense outer pyrolytic carbon (OPyC) layer with 40-μmnominal thickness. The TRISO-coated particle batches were sieved to upgrade the particles by removing over-sized and under-sized material, and the upgraded batches were designated by appending the letter A to the end of the batch number (e.g., 93165A).« less

AGR-1 Post Irradiation Examination Final Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Demkowicz, Paul Andrew

The post-irradiation examination (PIE) of the Advanced Gas Reactor (AGR)-1 experiment was a multi-year, collaborative effort between Idaho National Laboratory (INL) and Oak Ridge National Laboratory (ORNL) to study the performance of UCO (uranium carbide, uranium oxide) tristructural isotropic (TRISO) coated particle fuel fabricated in the U.S. and irradiated at the Advanced Test Reactor at INL to a peak burnup of 19.6% fissions per initial metal atom. This work involved a broad array of experiments and analyses to evaluate the level of fission product retention by the fuel particles and compacts (both during irradiation and during post-irradiation heating tests tomore » simulate reactor accident conditions), investigate the kernel and coating layer morphology evolution and the causes of coating failure, and explore the migration of fission products through the coating layers. The results have generally confirmed the excellent performance of the AGR-1 fuel, first indicated during the irradiation by the observation of zero TRISO coated particle failures out of 298,000 particles in the experiment. Overall release of fission products was determined by PIE to have been relatively low during the irradiation. A significant finding was the extremely low levels of cesium released through intact coatings. This was true both during the irradiation and during post-irradiation heating tests to temperatures as high as 1800°C. Post-irradiation safety test fuel performance was generally excellent. Silver release from the particles and compacts during irradiation was often very high. Extensive microanalysis of fuel particles was performed after irradiation and after high-temperature safety testing. The results of particle microanalysis indicate that the UCO fuel is effective at controlling the oxygen partial pressure within the particle and limiting kernel migration. Post-irradiation examination has provided the final body of data that speaks to the quality of the AGR-1 fuel

Commercial Spacewalking: Designing an EVA Qualification Program for Space Tourism

NASA Technical Reports Server (NTRS)

Gast, Matthew A.

2010-01-01

In the near future, accessibility to space will be opened to anyone with the means and the desire to experience the weightlessness of microgravity, and to look out upon both the curvature of the Earth and the blackness of space, from the protected, shirt-sleeved environment of a commercial spacecraft. Initial forays will be short-duration, suborbital flights, but the experience and expertise of half a century of spaceflight will soon produce commercial vehicles capable of achieving low Earth orbit. Even with the commercial space industry still in its infancy, and manned orbital flight a number of years away, there is little doubt that there will one day be a feasible and viable market for those courageous enough to venture outside the vehicle and into the void, wearing nothing but a spacesuit, armed with nothing but preflight training. What that Extravehicular Activity (EVA) preflight training entails, however, is something that has yet to be defined. A number of significant factors will influence the composition of a commercial EVA training program, but a fundamental question remains: 'what minimum training guidelines must be met to ensure a safe and successful commercial spacewalk?' Utilizing the experience gained through the development of NASA's Skills program - designed to qualify NASA and International Partner astronauts for EVA aboard the International Space Station - this paper identifies the attributes and training objectives essential to the safe conduct of an EVA, and attempts to conceptually design a comprehensive training methodology meant to represent an acceptable qualification standard.

Listeria monocytogenes differential transcriptome analysis reveals temperature-dependent Agr regulation and suggests overlaps with other regulons.

PubMed

Garmyn, Dominique; Augagneur, Yoann; Gal, Laurent; Vivant, Anne-Laure; Piveteau, Pascal

2012-01-01

Listeria monocytogenes is a ubiquitous, opportunistic pathogenic organism. Environmental adaptation requires constant regulation of gene expression. Among transcriptional regulators, AgrA is part of an auto-induction system. Temperature is an environmental cue critical for in vivo adaptation. In order to investigate how temperature may affect AgrA-dependent transcription, we compared the transcriptomes of the parental strain L. monocytogenes EGD-e and its ΔagrA mutant at the saprophytic temperature of 25°C and in vivo temperature of 37°C. Variations of transcriptome were higher at 37°C than at 25°C. Results suggested that AgrA may be involved in the regulation of nitrogen transport, amino acids, purine and pyrimidine biosynthetic pathways and phage-related functions. Deregulations resulted in a growth advantage at 37°C, but affected salt tolerance. Finally, our results suggest overlaps with PrfA, σB, σH and CodY regulons. These overlaps may suggest that through AgrA, Listeria monocytogenes integrates information on its biotic environment.

Listeria monocytogenes Differential Transcriptome Analysis Reveals Temperature-Dependent Agr Regulation and Suggests Overlaps with Other Regulons

PubMed Central

Garmyn, Dominique; Augagneur, Yoann; Gal, Laurent; Vivant, Anne-Laure; Piveteau, Pascal

2012-01-01

Listeria monocytogenes is a ubiquitous, opportunistic pathogenic organism. Environmental adaptation requires constant regulation of gene expression. Among transcriptional regulators, AgrA is part of an auto-induction system. Temperature is an environmental cue critical for in vivo adaptation. In order to investigate how temperature may affect AgrA-dependent transcription, we compared the transcriptomes of the parental strain L. monocytogenes EGD-e and its ΔagrA mutant at the saprophytic temperature of 25°C and in vivo temperature of 37°C. Variations of transcriptome were higher at 37°C than at 25°C. Results suggested that AgrA may be involved in the regulation of nitrogen transport, amino acids, purine and pyrimidine biosynthetic pathways and phage-related functions. Deregulations resulted in a growth advantage at 37°C, but affected salt tolerance. Finally, our results suggest overlaps with PrfA, σB, σH and CodY regulons. These overlaps may suggest that through AgrA, Listeria monocytogenes integrates information on its biotic environment. PMID:23024744

Analysis of tank 4 (FTF-4-15-22, 23) surface and subsurface supernatant samples in support of enrichment control, corrosion control and evaporator feed qualification programs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oji, L. N.

This report provides the results of analyses on Savannah River Site Tank 4 surface and subsurface supernatant liquid samples in support of the Enrichment Control Program (ECP), the Corrosion Control Program (CCP) and the Evaporator Feed Qualification (EFQ) Program. The purpose of the ECP sample taken from Tank 4 in August 2015 was to determine if the supernatant liquid would be “acceptable feed” to the 2H and 3H evaporator systems.

42 CFR 61.34 - Qualifications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 1 2011-10-01 2011-10-01 false Qualifications. 61.34 Section 61.34 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Service Fellowships § 61.34 Qualifications. Scholastic and other qualifications shall be...

42 CFR 61.34 - Qualifications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false Qualifications. 61.34 Section 61.34 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Service Fellowships § 61.34 Qualifications. Scholastic and other qualifications shall be...

42 CFR 61.34 - Qualifications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 1 2014-10-01 2014-10-01 false Qualifications. 61.34 Section 61.34 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Service Fellowships § 61.34 Qualifications. Scholastic and other qualifications shall be...

42 CFR 61.34 - Qualifications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 1 2012-10-01 2012-10-01 false Qualifications. 61.34 Section 61.34 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Service Fellowships § 61.34 Qualifications. Scholastic and other qualifications shall be...

42 CFR 61.34 - Qualifications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 1 2013-10-01 2013-10-01 false Qualifications. 61.34 Section 61.34 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Service Fellowships § 61.34 Qualifications. Scholastic and other qualifications shall be...

45 CFR 618.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 3 2014-10-01 2014-10-01 false Sex as a bona fide occupational qualification. 618... FOUNDATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

34 CFR 106.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 1 2010-07-01 2010-07-01 false Sex as a bona-fide occupational qualification. 106.61..., DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

34 CFR 106.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 34 Education 1 2013-07-01 2013-07-01 false Sex as a bona-fide occupational qualification. 106.61..., DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

34 CFR 106.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 34 Education 1 2012-07-01 2012-07-01 false Sex as a bona-fide occupational qualification. 106.61..., DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

34 CFR 106.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 1 2011-07-01 2011-07-01 false Sex as a bona-fide occupational qualification. 106.61..., DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

34 CFR 106.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 34 Education 1 2014-07-01 2014-07-01 false Sex as a bona-fide occupational qualification. 106.61..., DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

45 CFR 618.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Sex as a bona fide occupational qualification. 618... FOUNDATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

45 CFR 618.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 3 2011-10-01 2011-10-01 false Sex as a bona fide occupational qualification. 618... FOUNDATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

45 CFR 618.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 3 2012-10-01 2012-10-01 false Sex as a bona fide occupational qualification. 618... FOUNDATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

45 CFR 618.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 3 2013-10-01 2013-10-01 false Sex as a bona fide occupational qualification. 618... FOUNDATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

42 CFR 418.114 - Condition of participation: Personnel qualifications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 3 2011-10-01 2011-10-01 false Condition of participation: Personnel qualifications. 418.114 Section 418.114 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM HOSPICE CARE Conditions of participation...

Qualification test unit slide stainer (Beckman P/N 673753)

NASA Technical Reports Server (NTRS)

Bernier, P. S.

1972-01-01

Specifications for a slide stainer unit for the Skylab program are presented. The qualification test slide stainer was designed to be a self-contained system capable of performing an eight-step Gram stain of microorganisms and a Wright's stain of blood smears.

42 CFR 418.114 - Condition of participation: Personnel qualifications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 3 2014-10-01 2014-10-01 false Condition of participation: Personnel qualifications. 418.114 Section 418.114 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM (CONTINUED) HOSPICE CARE Conditions of...

42 CFR 418.114 - Condition of participation: Personnel qualifications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 3 2012-10-01 2012-10-01 false Condition of participation: Personnel qualifications. 418.114 Section 418.114 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM (CONTINUED) HOSPICE CARE Conditions of...

42 CFR 418.114 - Condition of participation: Personnel qualifications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 3 2013-10-01 2013-10-01 false Condition of participation: Personnel qualifications. 418.114 Section 418.114 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM (CONTINUED) HOSPICE CARE Conditions of...

The Development of a GaAs MMIC Reliability and Space Qualification Guide

NASA Technical Reports Server (NTRS)

Ponchak, G.; Kayali, S.; Huang, H-C.

1994-01-01

This paper discusses the need for a space qualification guide, provides a brief description of some common GaAs failure mechanisms, the approach that the NASA MMIC Reliability Assurance Program is following to develop the guide, and the status of the program.

49 CFR 25.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 1 2012-10-01 2012-10-01 false Sex as a bona fide occupational qualification. 25... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 25.550 Sex as a...

22 CFR 229.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Sex as a bona fide occupational qualification... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 229.550 Sex as...

49 CFR 25.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 1 2013-10-01 2013-10-01 false Sex as a bona fide occupational qualification. 25... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 25.550 Sex as a...

22 CFR 229.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Sex as a bona fide occupational qualification... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 229.550 Sex as...

22 CFR 146.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Sex as a bona fide occupational qualification... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.550 Sex as a...

22 CFR 146.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Sex as a bona fide occupational qualification... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.550 Sex as a...

22 CFR 146.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Sex as a bona fide occupational qualification... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.550 Sex as a...

49 CFR 25.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 1 2011-10-01 2011-10-01 false Sex as a bona fide occupational qualification. 25... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 25.550 Sex as a...

49 CFR 25.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Sex as a bona fide occupational qualification. 25... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 25.550 Sex as a...

22 CFR 146.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Sex as a bona fide occupational qualification... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.550 Sex as a...

22 CFR 229.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Sex as a bona fide occupational qualification... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 229.550 Sex as...

22 CFR 146.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Sex as a bona fide occupational qualification... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.550 Sex as a...

49 CFR 25.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 1 2014-10-01 2014-10-01 false Sex as a bona fide occupational qualification. 25... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 25.550 Sex as a...

22 CFR 229.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Sex as a bona fide occupational qualification... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 229.550 Sex as...

22 CFR 229.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Sex as a bona fide occupational qualification... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 229.550 Sex as...

Application of Microprocessor-Based Equipment in Nuclear Power Plants - Technical Basis for a Qualification Methodology

DOE Office of Scientific and Technical Information (OSTI.GOV)

Korsah, K.

This document (1) summarizes the most significant findings of the ''Qualification of Advanced Instrumentation and Control (I&C) Systems'' program initiated by the Nuclear Regulatory Commission (NRC); (2) documents a comparative analysis of U.S. and European qualification standards; and (3) provides recommendations for enhancing regulatory guidance for environmental qualification of microprocessor-based safety-related systems. Safety-related I&C system upgrades of present-day nuclear power plants, as well as I&C systems of Advanced Light-Water Reactors (ALWRs), are expected to make increasing use of microprocessor-based technology. The Nuclear Regulatory Commission (NRC) recognized that the use of such technology may pose environmental qualification challenges different from current,more » analog-based I&C systems. Hence, it initiated the ''Qualification of Advanced Instrumentation and Control Systems'' program. The objectives of this confirmatory research project are to (1) identify any unique environmental-stress-related failure modes posed by digital technologies and their potential impact on the safety systems and (2) develop the technical basis for regulatory guidance using these findings. Previous findings from this study have been documented in several technical reports. This final report in the series documents a comparative analysis of two environmental qualification standards--Institute of Electrical and Electronics Engineers (IEEE) Std 323-1983 and International Electrotechnical Commission (IEC) 60780 (1998)--and provides recommendations for environmental qualification of microprocessor-based systems based on this analysis as well as on the findings documented in the previous reports. The two standards were chosen for this analysis because IEEE 323 is the standard used in the U.S. for the qualification of safety-related equipment in nuclear power plants, and IEC 60780 is its European counterpart. In addition, the IEC document was published in 1998, and

Structure-Function Analysis of Peptide Signaling in the Clostridium perfringens Agr-Like Quorum Sensing System

PubMed Central

Ma, Menglin; Li, Jihong

2015-01-01

ABSTRACT The accessory growth regulator (Agr)-like quorum sensing (QS) system of Clostridium perfringens controls the production of many toxins, including beta toxin (CPB). We previously showed (J. E. Vidal, M. Ma, J. Saputo, J. Garcia, F. A. Uzal, and B. A. McClane, Mol Microbiol 83:179–194, 2012, http://dx.doi.org/10.1111/j.1365-2958.2011.07925.x) that an 8-amino-acid, AgrD-derived peptide named 8-R upregulates CPB production by this QS system. The current study synthesized a series of small signaling peptides corresponding to sequences within the C. perfringens AgrD polypeptide to investigate the C. perfringens autoinducing peptide (AIP) structure-function relationship. When both linear and cyclic ring forms of these peptides were added to agrB null mutants of type B strain CN1795 or type C strain CN3685, the 5-amino-acid peptides, whether in a linear or ring (thiolactone or lactone) form, induced better signaling (more CPB production) than peptide 8-R for both C. perfringens strains. The 5-mer thiolactone ring peptide induced faster signaling than the 5-mer linear peptide. Strain-related variations in sensing these peptides were detected, with CN3685 sensing the synthetic peptides more strongly than CN1795. Consistent with those synthetic peptide results, Transwell coculture experiments showed that CN3685 exquisitely senses native AIP signals from other isolates (types A, B, C, and D), while CN1795 barely senses even its own AIP. Finally, a C. perfringens AgrD sequence-based peptide with a 6-amino-acid thiolactone ring interfered with CPB production by several C. perfringens strains, suggesting potential therapeutic applications. These results indicate that AIP signaling sensitivity and responsiveness vary among C. perfringens strains and suggest C. perfringens prefers a 5-mer AIP to initiate Agr signaling. IMPORTANCE Clostridium perfringens possesses an Agr-like quorum sensing (QS) system that regulates virulence, sporulation, and toxin production. The

WTP Waste Feed Qualification: Glass Fabrication Unit Operation Testing Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stone, M. E.; Newell, J. D.; Johnson, F. C.

The waste feed qualification program is being developed to protect the Hanford Tank Waste Treatment and Immobilization Plant (WTP) design, safety basis, and technical basis by assuring waste acceptance requirements are met for each staged waste feed campaign prior to transfer from the Tank Operations Contractor to the feed receipt vessels inside the Pretreatment Facility. The Waste Feed Qualification Program Plan describes the three components of waste feed qualification: 1. Demonstrate compliance with the waste acceptance criteria 2. Determine waste processability 3. Test unit operations at laboratory scale. The glass fabrication unit operation is the final step in the processmore » demonstration portion of the waste feed qualification process. This unit operation generally consists of combining each of the waste feed streams (high-level waste (HLW) and low-activity waste (LAW)) with Glass Forming Chemicals (GFCs), fabricating glass coupons, performing chemical composition analysis before and after glass fabrication, measuring hydrogen generation rate either before or after glass former addition, measuring rheological properties before and after glass former addition, and visual observation of the resulting glass coupons. Critical aspects of this unit operation are mixing and sampling of the waste and melter feeds to ensure representative samples are obtained as well as ensuring the fabrication process for the glass coupon is adequate. Testing was performed using a range of simulants (LAW and HLW simulants), and these simulants were mixed with high and low bounding amounts of GFCs to evaluate the mixing, sampling, and glass preparation steps in shielded cells using laboratory techniques. The tests were performed with off-the-shelf equipment at the Savannah River National Laboratory (SRNL) that is similar to equipment used in the SRNL work during qualification of waste feed for the Defense Waste Processing Facility (DWPF) and other waste treatment facilities at

National Vocational Qualifications and the Construction Craft Industry.

ERIC Educational Resources Information Center

Further Education Unit, London (England).

Training and education in the construction craft industry for the 1990s and beyond will be affected by changes in industry, qualifications, and education and training provision. A shift away from apprenticeship programs toward shorter periods of training and further education has been accompanied by an increasing emphasis on the demonstration of…

The Diploma Qualification in England: An Avoidable Failure?

ERIC Educational Resources Information Center

Isaacs, Tina

2013-01-01

In 2005, the UK government introduced new vocationally related diploma qualifications. Two-year programs were examined in the summers of 2010 and 2011; initial government hopes for candidate numbers proved largely ephemeral and results were disappointing. This article explores what happened to the diploma's lofty ambitions using the summer of 2010…

Building Skills and Qualifications among SME Employees. Leonardo da Vinci Good Practices Series.

ERIC Educational Resources Information Center

Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.

This document profiles 10 European programs that exemplify good practice in building skills and qualifications among employees of small and medium enterprises (SMEs). The programs profiled are as follows: (1) TRICTSME (a program providing World Wide Web-based information and communication technologies training for SMEs in manufacturing); (2)…

Evaluation of the Adenocarcinoma-Associated Gene AGR2 and the Intestinal Stem Cell Marker LGR5 as Biomarkers in Colorectal Cancer

PubMed Central

Valladares-Ayerbes, Manuel; Blanco-Calvo, Moisés; Reboredo, Margarita; Lorenzo-Patiño, María J.; Iglesias-Díaz, Pilar; Haz, Mar; Díaz-Prado, Silvia; Medina, Vanessa; Santamarina, Isabel; Pértega, Sonia; Figueroa, Angélica; Antón-Aparicio, Luis M.

2012-01-01

We aim to estimate the diagnostic performances of anterior gradient homolog-2 (AGR2) and Leucine-rich repeat-containing-G-protein-coupled receptor 5 (LGR5) in peripheral blood (PB) as mRNA biomarkers in colorectal cancer (CRC) and to explore their prognostic significance. Real-time PCR was used to analyze AGR2 and LGR5 in 54 stages I-IV CRC patients and 19 controls. Both mRNAs were significantly increased in PB from CRC patients compared to controls. The area under the receiver-operating characteristic curves were 0.722 (p = 0.006), 0.376 (p = 0.123) and 0.767 (p = 0.001) for AGR2, LGR5 and combined AGR2/LGR5, respectively. The AGR2/LGR5 assay resulted in 67.4% sensitivity and 94.7% specificity. AGR2 correlated with pT3–pT4 and high-grade tumors. LGR5 correlated with metastasis, R2 resections and high-grade. The progression-free survival (PFS) of patients with high AGR2 was reduced (p = 0.037; HR, 2.32), also in the stage I-III subgroup (p = 0.046). LGR5 indicated a poor prognosis regarding both PFS (p = 0.007; HR, 1.013) and overall survival (p = 0.045; HR, 1.01). High AGR2/LGR5 was associated with poor PFS (p = 0.014; HR, 2.8) by multivariate analysis. Our findings indicate that the assessment of AGR2 and LGR5 in PB might reflect the presence of circulating tumor cells (CTC) and stem cell like CTC in CRC. Increased AGR2 and LGR5 are associated with poor outcomes. PMID:22605983

6 CFR 17.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 6 Domestic Security 1 2010-01-01 2010-01-01 false Sex as a bona fide occupational qualification... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 17.550 Sex as a...

6 CFR 17.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 6 Domestic Security 1 2013-01-01 2013-01-01 false Sex as a bona fide occupational qualification... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 17.550 Sex as a...

6 CFR 17.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 6 Domestic Security 1 2014-01-01 2014-01-01 false Sex as a bona fide occupational qualification... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 17.550 Sex as a...

6 CFR 17.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 6 Domestic Security 1 2011-01-01 2011-01-01 false Sex as a bona fide occupational qualification... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 17.550 Sex as a...

6 CFR 17.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 6 Domestic Security 1 2012-01-01 2012-01-01 false Sex as a bona fide occupational qualification... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 17.550 Sex as a...

20 CFR 411.235 - What qualifications are required of a PM?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false What qualifications are required of a PM? 411.235 Section 411.235 Employees' Benefits SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Use of One or More Program Managers To Assist in Administration of the Ticket to Work...

Joint Oil Analysis Program Spectrometer Standards SCP Science (Conostan) Qualification Report for D19-0, D3-100, and D12-XXX Series Standards

DTIC Science & Technology

2015-05-20

Joint Oil Analysis Program Spectrometer Standards SCP Science (Conostan) Qualification Report For D19-0, D3-100, and D12- XXX Series Standards NF...Candidate Type D19-0 ICP-AES Results ..................................................................... 4 Table V. Candidate Type D12- XXX ...Physical Property Results .................................................. 5 Table VI. Candidate Type D12- XXX Rotrode-AES Results

Guidelines for Selection, Screening and Qualification of Low-Voltage Commercial Multilayer Ceramic Capacitors for Space Programs

NASA Technical Reports Server (NTRS)

Teverovsky, Alexander A.

2012-01-01

This document has been developed in the course of NASA Electronic Parts and Packaging (NEPP) program and is not an official endorsement of the insertion of commercial capacitors in space programs or an established set of requirements for their testing. The purpose of this document is to suggest possible ways for selection, screening, and qualification of commercial capacitors for NASA projects and open discussions in the parts engineering community related to the use of COTS ceramic capacitors. This guideline is applicable to commercial surface mount chip, simple parallel plate design, multi-layer ceramic capacitors (MLCCs) rated to voltages of 100V and less. Parts with different design, e.g. low inductance ceramic capacitors (LICA), land grid array (LGA) etc., might need additional testing and tailoring of the requirements described in this document. Although the focus of this document is on commercial MLCCs, many procedures discussed below would be beneficial for military-grade capacitors

Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system.

PubMed

Zhou, Ying; Niu, Chao; Ma, Bo; Xue, Xiaoyan; Li, Zhi; Chen, Zhou; Li, Fen; Zhou, Shan; Luo, Xiaoxing; Hou, Zheng

2018-03-02

Given its high resistance, enhanced virulence, and high transmissibility, community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) pneumonia is highly associated with high morbidity and mortality. Anti-virulence therapy is a promising strategy that bypasses the evolutionary pressure on the bacterium to develop resistance. RNAIII-inhibiting peptide (RIP), as an accessory gene regulator (agr)-specific inhibitor, significantly restricts the virulence of S. aureus and protects infected mice from death by blocking the agr quorum sensing system. The protective effects of RIP on the neutropenic mice completely disappeared in a neutrophil-deleted mouse infection model, but not in the macrophage-deleted mice. This result confirmed that the in vivo antibacterial activity of RIP is highly associated with neutrophil function. Phenol-soluble modulins (PSMs), as major leukocyte lysis toxins of CA-MRSA, are directly regulated by the agr system. In this experiment, PSMα1, 2, and 3 significantly induced neutrophil necroptosis by activating mixed lineage kinase-like protein (MLKL) phosphorylation and increasing lactate dehydrogenase release. The S. aureus supernatants harvested from the agr or psmα mutant strains both decreased the phosphorylation level of MLKL and cell lysis. PSMα1-mediated neutrophil lysis was significantly inhibited by necrosulfonamide, necrostatin-1, TNFα antibody, and WRW4. These results showed PSMα1 induced necroptosis depends on formylpeptide receptor 2 (FPR2)-mediated autocrine TNFα. Moreover, the neutrophil necroptosis induced by S. aureus was significantly suppressed and pneumonia was effectively prevented by the blockage of agrA and psmα expression levels. These findings indicate that PSMα-induced necroptosis is a major cause of lung pathology in S. aureus pneumonia and suggest that interfering with the agr quorum sensing signaling pathway is a potential therapeutic strategy.

45 CFR 86.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 1 2013-10-01 2013-10-01 false Sex as a bona-fide occupational qualification. 86... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 86.61 Sex as a...

45 CFR 86.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Sex as a bona-fide occupational qualification. 86... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 86.61 Sex as a...

45 CFR 86.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 1 2011-10-01 2011-10-01 false Sex as a bona-fide occupational qualification. 86... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 86.61 Sex as a...

45 CFR 86.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 1 2014-10-01 2014-10-01 false Sex as a bona-fide occupational qualification. 86... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 86.61 Sex as a...

45 CFR 86.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 1 2012-10-01 2012-10-01 false Sex as a bona-fide occupational qualification. 86... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 86.61 Sex as a...

The Sequence-specific Peptide-binding Activity of the Protein Sulfide Isomerase AGR2 Directs Its Stable Binding to the Oncogenic Receptor EpCAM.

PubMed

Mohtar, M Aiman; Hernychova, Lenka; O'Neill, J Robert; Lawrence, Melanie L; Murray, Euan; Vojtesek, Borek; Hupp, Ted R

2018-04-01

AGR2 is an oncogenic endoplasmic reticulum (ER)-resident protein disulfide isomerase. AGR2 protein has a relatively unique property for a chaperone in that it can bind sequence-specifically to a specific peptide motif (TTIYY). A synthetic TTIYY-containing peptide column was used to affinity-purify AGR2 from crude lysates highlighting peptide selectivity in complex mixtures. Hydrogen-deuterium exchange mass spectrometry localized the dominant region in AGR2 that interacts with the TTIYY peptide to within a structural loop from amino acids 131-135 (VDPSL). A peptide binding site consensus of Tx[IL][YF][YF] was developed for AGR2 by measuring its activity against a mutant peptide library. Screening the human proteome for proteins harboring this motif revealed an enrichment in transmembrane proteins and we focused on validating EpCAM as a potential AGR2-interacting protein. AGR2 and EpCAM proteins formed a dose-dependent protein-protein interaction in vitro Proximity ligation assays demonstrated that endogenous AGR2 and EpCAM protein associate in cells. Introducing a single alanine mutation in EpCAM at Tyr251 attenuated its binding to AGR2 in vitro and in cells. Hydrogen-deuterium exchange mass spectrometry was used to identify a stable binding site for AGR2 on EpCAM, adjacent to the TLIYY motif and surrounding EpCAM's detergent binding site. These data define a dominant site on AGR2 that mediates its specific peptide-binding function. EpCAM forms a model client protein for AGR2 to study how an ER-resident chaperone can dock specifically to a peptide motif and regulate the trafficking a protein destined for the secretory pathway. © 2018 by The American Society for Biochemistry and Molecular Biology, Inc.

5 CFR 302.403 - Qualifications for promotion.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Qualifications for promotion. 302.403... EMPLOYMENT IN THE EXCEPTED SERVICE Selection and Appointment; Reappointment; and Qualifications for Promotion § 302.403 Qualifications for promotion. In determining qualifications for promotion with respect to an...

Qualification test and analysis report: Solar collectors

NASA Technical Reports Server (NTRS)

1978-01-01

Test results show that the Owens-Illinois Sunpak TM Model SEC 601 air-cooled collector meets the national standards and codes as defined in the Subsystem Peformance Specification and Verification Plan of NASA/MSFC, dated October 28, 1976. The program calls for the development, fabrication, qualification and delivery of an air-cooled solar collector for solar heating, combined heating and cooling, and/or hot water systems.

Building world-class microlithographic lens systems: optical material requirements and qualification methods

NASA Astrophysics Data System (ADS)

DeStefano, Paul R.; Michaloski, Paul F.

1993-12-01

Building successive generations of state-of-the-art wide field, sub-micron microlithographic lens systems dictates ever-tightening material tolerances that challenge glass manufacturers. This paper discusses the optical material needs for microlithographic lens systems and Tropel's in-house material qualification program. Material qualification is divided into three successive stages: (1) fluorescence testing to qualitatively analyze color center characteristics of the material; (2) homogeneity testing to determine the relative volumetric variations in index; and (3) absolute index testing at multiple wavelengths to determine the material's dispersion characteristics.

ELECTRON PROBE MICROANALYSIS OF IRRADIATED AND 1600°C SAFETY-TESTED AGR-1 TRISO FUEL PARTICLES WITH LOW AND HIGH RETAINED 110MAG

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wright, Karen E.; van Rooyen, Isabella J.

2016-11-01

AGR-1 fuel Compact 4-3-3 achieved 18.63% FIMA and was exposed subsequently to a safety test at 1600°C. Two particles, AGR1-433-003 and AGR1-433-007, with measured-to-calculated 110mAg inventories of <22% and 100%, respectively, were selected for comparative electron microprobe analysis to determine whether the distribution or abundance of fission products differed proximally and distally from the deformed kernel in AGR1-433-003, and how this compared to fission product distribution in AGR1-433-007. On the deformed side of AGR1-433-003, Xe, Cs, I, Eu, Sr, and Te concentrations in the kernel buffer interface near the protruded kernel were up to six times higher than on themore » opposite, non-deformed side. At the SiC-inner pyrolytic carbon (IPyC) interface proximal to the deformed kernel, Pd and Ag concentrations were 1.2 wt% and 0.04 wt% respectively, whereas on the SiC-IPyC interface distal from the kernel deformation those elements measured 0.4 and 0.01 wt%, respectively. Palladium and Ag concentrations at the SiC-IPyC interface of AGR1-433-007 were 2.05 and 0.05 wt.%, respectively. Rare earth element concentrations at the SiC-IPyC interface of AGR1-433-007 were a factor of ten higher than at the SiC-IPyC interfaces measured in particle AGR1-433-003. Palladium permeated the SiC layer of AGR1-433-007 and the non-deformed SiC layer of AGR1-433-003.« less

34 CFR 300.156 - Personnel qualifications.

Code of Federal Regulations, 2011 CFR

2011-07-01

... children with disabilities. (c) Qualifications for special education teachers. The qualifications described... personnel to provide special education and related services under this part to children with disabilities... 34 Education 2 2011-07-01 2010-07-01 true Personnel qualifications. 300.156 Section 300.156...

5 CFR 319.302 - Individual qualifications.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Individual qualifications. 319.302... Individual qualifications. Agency heads are delegated authority to approve the qualifications of individuals appointed to SL and ST positions. The agency head must determine that the individual meets the...

10 CFR 603.510 - Recipient qualifications.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Recipient qualifications. 603.510 Section 603.510 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS TECHNOLOGY INVESTMENT AGREEMENTS Pre-Award Business Evaluation Recipient Qualification § 603.510 Recipient qualifications. Prior to award of a TIA, the...

10 CFR 603.510 - Recipient qualifications.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Recipient qualifications. 603.510 Section 603.510 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS TECHNOLOGY INVESTMENT AGREEMENTS Pre-Award Business Evaluation Recipient Qualification § 603.510 Recipient qualifications. Prior to award of a TIA, the...

Specification for Qualification and Certification for Entry Level Welders.

ERIC Educational Resources Information Center

American Welding Society, Miami, FL.

This document provides a standard that defines the requirements and program for the American Welding Society to certify entry-level welders. The certification of entry-level welders requires performance qualification and practical knowledge tests that require a minimum of reading, computation, and manual skills to complete. The Entry-Level Welder…

Payload test philosophy. [JPL views on qualification/acceptance testing

NASA Technical Reports Server (NTRS)

Gindorf, T.

1979-01-01

The general philosophy of how JPL views payload qualification/acceptance testing for programs that are done either in-house or by contractors is described. Particular attention is given to mission risk classifications, preliminary critical design reviews, environmental design requirements, the thermal and dynamics development tests, and the flight spacecraft system test.

Proposed acceptance, qualification, and characterization tests for thin-film PV modules

NASA Technical Reports Server (NTRS)

Waddington, D.; Mrig, L.; Deblasio, R.; Ross, R.

1988-01-01

Details of a proposed test program for PV thin-film modules which the Department of Energy has directed the Solar Energy Research Institute (SERI) to prepare are presented. Results of one of the characterization tests that SERI has performed are also presented. The objective is to establish a common approach to testing modules that will be acceptable to both users and manufacturers. The tests include acceptance, qualification, and characterization tests. Acceptance tests verify that randomly selected modules have similar characteristics. Qualification tests are based on accelerated test methods designed to simulate adverse conditions. Characterization tests provide data on performance in a predefined environment.

Pro-metastatic activity of AGR2 interrupts angiogenesis target bevacizumab efficiency via direct interaction with VEGFA and activation of NF-κB pathway.

PubMed

Jia, Mengqi; Guo, Yanxia; Zhu, Deyu; Zhang, Nianzhao; Li, Lin; Jiang, Jin; Dong, Yiwen; Xu, Qingqing; Zhang, Xiulei; Wang, Meijuan; Yu, Haina; Wang, Fang; Tian, Keli; Zhang, Jinsan; Young, Charles Y F; Lou, Hongxiang; Yuan, Huiqing

2018-05-01

Anterior gradient 2 (AGR2), an endoplasmic reticulum (ER)-resident protein-disulfide isomerase (PDI), is associated with cancer development and malignant progression. Here, we show that high level of AGR2 promotes the aggressive phenotype of prostate cancer (PCa) mouse models developed by either patient-derived xenografts or surgical intra-prostate implantation of PCa cells, associated with enrichment of the blood vessel network in tumor tissues. Angiogenesis markers VEGFR2 and CD34, accompanied with the invasive marker Vimentin, were predominantly stained in metastatic liver tissues. Secreted AGR2 was defined to enhance VEGFR2 activity as evidenced by physical interaction of purified recombinant human AGR2 (rhAGR2) with rhVEGFA through the formation of a disulfide bond. Mutant or deleted thioredoxin motif in rhAGR2 was also unable to bind to rhVEGFA that led to the significant abolishment in the vessel formation, but partially affecting the aggressive process, implicating alternative mechanisms are required for AGR2-conferring metastasis. Cytosolic AGR2 contributed to cell metastasis ascribed to its stabilizing effect on p65 protein, which subsequently activated the NF-κB and facilitated epithelial to mesenchymal transition (EMT). Importantly, GSH and cabozantinib, but not bevacizumab, effectively blocked the pro-angiogenic effect of rhAGR2 in vitro and in vivo, providing evidence that secreted AGR2 acts as a predictive biomarker for selection of angiogenesis-targeting therapeutic drugs based on its levels in the circular system. Copyright © 2018 Elsevier B.V. All rights reserved.

29 CFR 32.14 - Job qualifications.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 1 2014-07-01 2013-07-01 true Job qualifications. 32.14 Section 32.14 Labor Office of the... FINANCIAL ASSISTANCE Employment Practices and Employment Related Training Participation § 32.14 Job... appropriateness of all job qualifications to ensure that to the extent job qualifications tend to exclude...

29 CFR 32.14 - Job qualifications.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 1 2013-07-01 2013-07-01 false Job qualifications. 32.14 Section 32.14 Labor Office of the... FINANCIAL ASSISTANCE Employment Practices and Employment Related Training Participation § 32.14 Job... appropriateness of all job qualifications to ensure that to the extent job qualifications tend to exclude...

29 CFR 32.14 - Job qualifications.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 1 2010-07-01 2010-07-01 true Job qualifications. 32.14 Section 32.14 Labor Office of the... FINANCIAL ASSISTANCE Employment Practices and Employment Related Training Participation § 32.14 Job... appropriateness of all job qualifications to ensure that to the extent job qualifications tend to exclude...

29 CFR 32.14 - Job qualifications.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 1 2012-07-01 2012-07-01 false Job qualifications. 32.14 Section 32.14 Labor Office of the... FINANCIAL ASSISTANCE Employment Practices and Employment Related Training Participation § 32.14 Job... appropriateness of all job qualifications to ensure that to the extent job qualifications tend to exclude...

29 CFR 32.14 - Job qualifications.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 1 2011-07-01 2011-07-01 false Job qualifications. 32.14 Section 32.14 Labor Office of the... FINANCIAL ASSISTANCE Employment Practices and Employment Related Training Participation § 32.14 Job... appropriateness of all job qualifications to ensure that to the extent job qualifications tend to exclude...

10 CFR 603.510 - Recipient qualifications.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Evaluation Recipient Qualification § 603.510 Recipient qualifications. Prior to award of a TIA, the... for awarding a grant or cooperative agreement. If the recipient is a consortium that is not formally... 10 Energy 4 2014-01-01 2014-01-01 false Recipient qualifications. 603.510 Section 603.510 Energy...

10 CFR 603.510 - Recipient qualifications.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Evaluation Recipient Qualification § 603.510 Recipient qualifications. Prior to award of a TIA, the... for awarding a grant or cooperative agreement. If the recipient is a consortium that is not formally... 10 Energy 4 2011-01-01 2011-01-01 false Recipient qualifications. 603.510 Section 603.510 Energy...

10 CFR 603.510 - Recipient qualifications.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Evaluation Recipient Qualification § 603.510 Recipient qualifications. Prior to award of a TIA, the... for awarding a grant or cooperative agreement. If the recipient is a consortium that is not formally... 10 Energy 4 2012-01-01 2012-01-01 false Recipient qualifications. 603.510 Section 603.510 Energy...

Cambios al Estándar de Protección a los Trabajadores Agrícolas de la EPA

EPA Pesticide Factsheets

Estos cambios proporcionarán protecciones de salud a los trabajadores agrícolas similares a las que ya disponen trabajadores en otras industrias, teniendo en cuenta el entorno laboral único de muchos trabajos agrícolas.

Specification for Qualification and Certification for Level II - Advanced Welders.

ERIC Educational Resources Information Center

American Welding Society, Miami, FL.

This document defines the requirements and program for the American Welding Society (AWS) to certify advanced-level welders through an evaluation process entailing performance qualification and practical knowledge tests requiring the use of advanced reading, computational, and manual skills. The following items are included: statement of the…

13 CFR 120.1703 - Qualifications to be a Pool Originator.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Qualifications to be a Pool Originator. 120.1703 Section 120.1703 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan Pools...

13 CFR 120.1703 - Qualifications to be a Pool Originator.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Qualifications to be a Pool Originator. 120.1703 Section 120.1703 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan Pools...

13 CFR 120.1703 - Qualifications to be a Pool Originator.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Qualifications to be a Pool Originator. 120.1703 Section 120.1703 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan Pools...

13 CFR 120.1703 - Qualifications to be a Pool Originator.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Qualifications to be a Pool Originator. 120.1703 Section 120.1703 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan Pools...

76 FR 29335 - Qualification, Service, and Use of Crewmembers and Aircraft Dispatchers

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-20

...On January 12, 2009, the FAA published a notice of proposed rulemaking on qualification, service, and use of crewmembers and aircraft dispatchers. Because of the complexity of the issues and the concerns raised by commenters, the FAA is issuing this supplemental notice of proposed rulemaking. The FAA proposes to amend the regulations for crewmember and aircraft dispatcher training programs in domestic, flag, and supplemental operations. The proposed regulations enhance traditional training programs by requiring the use of flight simulation training devices for flightcrew members and including additional training and evaluation requirements for all crewmembers and aircraft dispatchers in areas that are critical to safety. The proposal also reorganizes and revises the qualification, training, and evaluation requirements. The proposed changes are intended to contribute significantly to reducing aviation accidents.

SP-100 flight qualification testing assessment

NASA Technical Reports Server (NTRS)

Jeanmougin, Nanette M.; Moore, Roger M.; Wait, David L.; Jacox, Michael G.

1988-01-01

The SP-100 is a compact space power system driven by a nuclear reactor that provides 100 kWe to the user at 200 VDC. The thermal energy generated by the nuclear reactor is converted into electrical energy by passive thermoelectric devices. Various options for tailoring the MIL-STD-1540B guidelines to the SP-100 nuclear power system are discussed. This study aids in selecting the appropriate qualification test program based on the cost, schedule, and test effectiveness of the various options.

BSA Delta Qualification 2, volume 3, book 1

NASA Technical Reports Server (NTRS)

1994-01-01

This report, presented in three volumes, provides the results of a two-motor Delta Qualification 2 program conducted in 1993 to certify the following enhancements for incorporation into booster separation motor (BSM) flight hardware: vulcanized-in-place nozzle aft closure insulation; new iso-static ATJ bulk graphite throat insert material; adhesive EA 9394 for bonding the nozzle throat, igniter grain rod/centering insert/igniter case; deletion of the igniter adapter insulator ring; deletion of the igniter adapter/igniter case interface RTV; and deletion of Loctite from igniter retainer plate threads. The enhancements above directly resulted from (1) the BSM total quality management (TQM) team initiatives to enhance the BSM producibility, and (2) the necessity to qualify new throat insert and adhesive systems to replace existing materials that will not be available. Testing was completed at both the component and motor levels. Component testing was accomplished to screen candidate materials (e.g., throat materials, adhesive systems) and to optimize processes (e.g., aft closure insulator vulcanization approach) prior to their incorporation into the test motors. Motor tests -- consisting of two motors, randomly selected by USBI's on-site quality personnel from production lot AAY, which were modified to accept the enhancements -- were completed to provide the final qualification of the enhancements for incorporation into flight hardware. Volume 3 book 1 provides supporting documentation to the analyses and plans of testing the two Delta Qualification units including thermal cycling planning/data acceptance records, environmental test procedures and pretest temperature conditioning history, Delta Qualification test plan, and specification SE0837 -- mix acceptance test specification.

Loss of Anterior Gradient 2 (Agr2) Expression Results in Hyperplasia and Defective Lineage Maturation in the Murine Stomach*

PubMed Central

Gupta, Aparna; Wodziak, Dariusz; Tun, May; Bouley, Donna M.; Lowe, Anson W.

2013-01-01

Recent studies of epithelial tissues have revealed the presence of tissue-specific stem cells that are able to establish multiple cell lineages within an organ. The stem cells give rise to progenitors that replicate before differentiating into specific cell lineages. The mechanism by which homeostasis is established between proliferating stem or progenitor cells and terminally differentiated cells is unclear. This study demonstrates that Agr2 expression by mucous neck cells in the stomach promotes the differentiation of multiple cell lineages while also inhibiting the proliferation of stem or progenitor cells. When Agr2 expression is absent, gastric mucous neck cells increased in number as does the number of proliferating cells. Agr2 expression loss also resulted in the decline of terminally differentiated cells, which was supplanted by cells that exhibited nuclear SOX9 labeling. Sox9 expression has been associated with progenitor and stem cells. Similar effects of the Agr2 null on cell proliferation in the intestine were also observed. Agr2 consequently serves to maintain the balance between proliferating and differentiated epithelial cells. PMID:23209296

HIGH-TEMPERATURE SAFETY TESTING OF IRRADIATED AGR-1 TRISO FUEL

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stempien, John D.; Demkowicz, Paul A.; Reber, Edward L.

High-Temperature Safety Testing of Irradiated AGR-1 TRISO Fuel John D. Stempien, Paul A. Demkowicz, Edward L. Reber, and Cad L. Christensen Idaho National Laboratory, P.O. Box 1625 Idaho Falls, ID 83415, USA Corresponding Author: john.stempien@inl.gov, +1-208-526-8410 Two new safety tests of irradiated tristructural isotropic (TRISO) coated particle fuel have been completed in the Fuel Accident Condition Simulator (FACS) furnace at the Idaho National Laboratory (INL). In the first test, three fuel compacts from the first Advanced Gas Reactor irradiation experiment (AGR-1) were simultaneously heated in the FACS furnace. Prior to safety testing, each compact was irradiated in the Advanced Testmore » Reactor to a burnup of approximately 15 % fissions per initial metal atom (FIMA), a fast fluence of 3×1025 n/m2 (E > 0.18 MeV), and a time-average volume-average (TAVA) irradiation temperature of about 1020 °C. In order to simulate a core-conduction cool-down event, a temperature-versus-time profile having a peak temperature of 1700 °C was programmed into the FACS furnace controllers. Gaseous fission products (i.e., Kr-85) were carried to the Fission Gas Monitoring System (FGMS) by a helium sweep gas and captured in cold traps featuring online gamma counting. By the end of the test, a total of 3.9% of an average particle’s inventory of Kr-85 was detected in the FGMS traps. Such a low Kr-85 activity indicates that no TRISO failures (failure of all three TRISO layers) occurred during the test. If released from the compacts, condensable fission products (e.g., Ag-110m, Cs-134, Cs-137, Eu-154, Eu-155, and Sr-90) were collected on condensation plates fitted to the end of the cold finger in the FACS furnace. These condensation plates were then analyzed for fission products. In the second test, five loose UCO fuel kernels, obtained from deconsolidated particles from an irradiated AGR-1 compact, were heated in the FACS furnace to a peak temperature of 1600 °C. This test

Specification for Qualification and Certification for Level III - Expert Welders.

ERIC Educational Resources Information Center

American Welding Society, Miami, FL.

This document defines the requirements and program for the American Welding Society to certify expert welders through an evaluation process entailing performance qualification and practical knowledge tests requiring the use of advanced reading, computational, and manual skills. The following items are included: statement of the standard's scope;…

16 CFR 254.5 - Misrepresentations of enrollment qualifications or limitations.

Code of Federal Regulations, 2014 CFR

2014-01-01

... qualifications for enrollment in a school or program of instruction. (b) It is deceptive for an Industry Member to misrepresent, directly or indirectly, expressly or by implication, that the lack of a high school... PRACTICE RULES GUIDES FOR PRIVATE VOCATIONAL AND DISTANCE EDUCATION SCHOOLS § 254.5 Misrepresentations of...

The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model.

PubMed

Gong, Jian; Li, Dongzhi; Yan, Jun; Liu, Yu; Li, Di; Dong, Jie; Gao, Yaping; Sun, Tao; Yang, Guang

2014-01-01

Intracranial abscesses are associated with high mortality. Staphylococcus aureus is one of the main pathogens that cause intracranial infection. Until now, there is no report to identify the key effectors of S. aureus during the intracranial infection. The murine intracranial abscesses model induced by S. aureus was constructed. The vital sign and survival rate of mice were observed to evaluate the infection. Histological examination was used to diagnose the pathological alterations of mouse tissues. The sensitivity of S. aureus to whole blood was evaluated by whole-blood killing assay. In murine intracranial abscesses model, it was shown that the mortality caused by the accessory gene regulator (agr) locus deficient strain was significant decreased compared with its parent strain. Moreover, we found that RNAIII, the effector of agr system, was essential for the intracranial infection caused by S. aureus. In the further investigation, it was shown that restoration the expression of α-toxin in agr deficient strain could partially recover the mortality in the murine intracranial abscesses model. Our data suggested that the agr system of S. aureus is an important virulence determinant in the induction and mortality of intracranial abscesses in mice. Copyright © 2014 Elsevier Editora Ltda. All rights reserved.

14 CFR 60.27 - Automatic loss of qualification and procedures for restoration of qualification.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Automatic loss of qualification and procedures for restoration of qualification. 60.27 Section 60.27 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

14 CFR 60.29 - Other losses of qualification and procedures for restoration of qualification.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Other losses of qualification and procedures for restoration of qualification. 60.29 Section 60.29 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

14 CFR 60.27 - Automatic loss of qualification and procedures for restoration of qualification.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Automatic loss of qualification and procedures for restoration of qualification. 60.27 Section 60.27 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

14 CFR 60.29 - Other losses of qualification and procedures for restoration of qualification.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Other losses of qualification and procedures for restoration of qualification. 60.29 Section 60.29 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

14 CFR 60.29 - Other losses of qualification and procedures for restoration of qualification.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Other losses of qualification and procedures for restoration of qualification. 60.29 Section 60.29 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

14 CFR 60.29 - Other losses of qualification and procedures for restoration of qualification.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Other losses of qualification and procedures for restoration of qualification. 60.29 Section 60.29 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

14 CFR 60.27 - Automatic loss of qualification and procedures for restoration of qualification.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Automatic loss of qualification and procedures for restoration of qualification. 60.27 Section 60.27 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

14 CFR 60.29 - Other losses of qualification and procedures for restoration of qualification.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Other losses of qualification and procedures for restoration of qualification. 60.29 Section 60.29 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

14 CFR 60.27 - Automatic loss of qualification and procedures for restoration of qualification.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Automatic loss of qualification and procedures for restoration of qualification. 60.27 Section 60.27 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

14 CFR 60.27 - Automatic loss of qualification and procedures for restoration of qualification.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Automatic loss of qualification and procedures for restoration of qualification. 60.27 Section 60.27 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

49 CFR 391.41 - Physical qualifications for drivers.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 5 2012-10-01 2012-10-01 false Physical qualifications for drivers. 391.41... QUALIFICATIONS OF DRIVERS AND LONGER COMBINATION VEHICLE (LCV) DRIVER INSTRUCTORS Physical Qualifications and Examinations § 391.41 Physical qualifications for drivers. (a)(1)(i) A person subject to this part must not...

49 CFR 391.41 - Physical qualifications for drivers.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 5 2011-10-01 2011-10-01 false Physical qualifications for drivers. 391.41... QUALIFICATIONS OF DRIVERS AND LONGER COMBINATION VEHICLE (LCV) DRIVER INSTRUCTORS Physical Qualifications and Examinations § 391.41 Physical qualifications for drivers. (a)(1)(i) A person subject to this part must not...

49 CFR 391.41 - Physical qualifications for drivers.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 5 2013-10-01 2013-10-01 false Physical qualifications for drivers. 391.41... QUALIFICATIONS OF DRIVERS AND LONGER COMBINATION VEHICLE (LCV) DRIVER INSTRUCTORS Physical Qualifications and Examinations § 391.41 Physical qualifications for drivers. (a)(1)(i) A person subject to this part must not...

49 CFR 391.41 - Physical qualifications for drivers.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 5 2014-10-01 2014-10-01 false Physical qualifications for drivers. 391.41... QUALIFICATIONS OF DRIVERS AND LONGER COMBINATION VEHICLE (LCV) DRIVER INSTRUCTORS Physical Qualifications and Examinations § 391.41 Physical qualifications for drivers. (a)(1)(i) A person subject to this part must not...

An Evaluation of Instructor Qualifications for Teaching Dual Enrollment Classes

ERIC Educational Resources Information Center

Abdul-Karim, Barbara Daaiyah

2010-01-01

This study examined instructor qualifications for teaching dual enrollment students, college-level work. This research focused on Maryland dual enrollment programs that offered both high school and college credit to high school students for each college Associate of Arts and Associate of Science transfer course taken. One significant goal of dual…

32 CFR 196.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 2 2011-07-01 2011-07-01 false Sex as a bona fide occupational qualification... DEFENSE (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

32 CFR 196.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 2 2014-07-01 2014-07-01 false Sex as a bona fide occupational qualification... DEFENSE (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

32 CFR 196.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Sex as a bona fide occupational qualification... DEFENSE (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

32 CFR 196.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 2 2013-07-01 2013-07-01 false Sex as a bona fide occupational qualification... DEFENSE (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

32 CFR 196.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 2 2012-07-01 2012-07-01 false Sex as a bona fide occupational qualification... DEFENSE (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

14 CFR 121.915 - Continuing qualification curriculum.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Continuing qualification curriculum. 121... Continuing qualification curriculum. Each continuing qualification curriculum must contain training and... training device as approved in the certificate holder's curriculum that must be completed during each...

49 CFR 180.507 - Qualification of tank cars.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Qualification of tank cars. 180.507 Section 180... QUALIFICATION AND MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.507 Qualification of tank cars. (a) Each tank car marked as meeting a “DOT” specification or any other tank car used...

Social Partnership in Accrediting Lithuanian VET Qualifications

ERIC Educational Resources Information Center

Tutlys, Vidmantas; Kaminskiene, Lina

2008-01-01

This article examines social partnership in accrediting qualifications in Lithuania. It defines the factors influencing social partnership and surveys future development perspectives, referring to the creation and implementation of the national qualifications system in Lithuania. Social partnership in qualifications accreditation is regarded as a…

Qualification testing of flat-plate photovoltaic modules

NASA Technical Reports Server (NTRS)

Hoffman, A. R.; Griffith, J. S.; Ross, R. G., Jr.

1982-01-01

The placement of photovoltaic modules in various applications, in climates and locations throughout the world, results in different degrees and combinations of environmental and electrical stress. Early detection of module reliability deficiencies via laboratory testing is necessary for achieving long, satisfactory field service. This overview paper describes qualification testing techniques being used in the US Department of Energy's flat-plate terrestrial photovoltaic development program in terms of their significance, rationale for specified levels and durations, and test results.

The European Medicines Agency experience with biomarker qualification.

PubMed

Manolis, Efthymios; Koch, Armin; Deforce, Dieter; Vamvakas, Spiros

2015-01-01

Since the launch of the qualification process in 2009, the CHMP reviewed/is reviewing 48 requests for qualification advice or opinion (as of Sept 2013) related to biomarkers (BM) or other novel drug development tools (e.g. patient reported outcome measures, modeling, and statistical methods). The qualification opinions are available on the EMA website (Qualification of novel methodologies for medicine development, http://www.ema.europa.eu/ema/index.jsp?curl=pages/regulation/document_listing/document_listing_000319.jsp&mid=WC0b01ac0580022bb0#section2 , 2013). Also there is a trend of increasing numbers of qualification requests to CHMP, indicative of the pace that targeted drug development and personalized medicine is gaining and the need to bring the new tools from research to drug development and clinical use. This chapter will focus on the regulatory experience gained so far from the CHMP qualification procedure. Basic qualification principles will be presented. Through qualification examples, we will elaborate on common grounds and divergences between the different stakeholders.

Round table discussion " Development of qualification framework in meteorology (TEMPUS QUALIMET)"

NASA Astrophysics Data System (ADS)

Bashmakova, I.; Belotserkovsky, A.; Karlin, L.; Petrosyan, A.; Serditova, N.; Zilitinkevich, S.

2010-09-01

The international consortium has started implementing a project aimed at the development of unified framework of qualifications in meteorology (QualiMet), setting a system of recognition and award of qualifications up to Doctoral level based on standards of knowledge, skill and competence acquired by learners is underway. The QualiMet has the following specific objectives: 1. To develop standards of knowledge, skills and competence for all qualifications up to Doctoral level needed in all possible occupations meteorology learner can undertake, by July 2011 2. To develop reciprocally recognized rubrics, criteria, methods and tools for assessing the compliance with the developed standards (quality assurance), by July 2012 3. To set the network of Centers of Excellence as the primary designer of sample education programs and learning experiences, both in brick-and-mortar and distant setting of delivery, leading to achievement of the developed standards, by December 2012 4. To set a system of mutual international recognition and award of qualifications in meteorology based on the developed procedures and establishment of self-regulatory public organization, by December 2012 The main beneficiaries of the project are: 1. Meteorology learners from the consortium countries. They will be able to make informed decisions about available qualification choices and progression options and provided an opportunity for students and graduates to participate in the system of international continuous education. 2. Meteorology employers from the consortium countries, They will be able to specify the level of knowledge, skill and competence required for occupational roles, evaluate qualifications presented, connect training and development with business needs. 3. Students and academic staff of all the consortium members, who will gain the increased mobility and exchange the fluxes of culturally and institutionally diversified lecturers and qualified specialists

45 CFR 2555.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 4 2014-10-01 2014-10-01 false Sex as a bona fide occupational qualification... FOR NATIONAL AND COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

45 CFR 2555.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 4 2013-10-01 2013-10-01 false Sex as a bona fide occupational qualification... FOR NATIONAL AND COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

45 CFR 2555.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 4 2010-10-01 2010-10-01 false Sex as a bona fide occupational qualification... FOR NATIONAL AND COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

45 CFR 2555.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 4 2012-10-01 2012-10-01 false Sex as a bona fide occupational qualification... FOR NATIONAL AND COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

45 CFR 2555.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 4 2011-10-01 2011-10-01 false Sex as a bona fide occupational qualification... FOR NATIONAL AND COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

MODELING AND ANALYSIS OF FISSION PRODUCT TRANSPORT IN THE AGR-3/4 EXPERIMENT

DOE Office of Scientific and Technical Information (OSTI.GOV)

Humrickhouse, Paul W.; Collin, Blaise P.; Hawkes, Grant L.

In this work we describe the ongoing modeling and analysis efforts in support of the AGR-3/4 experiment. AGR-3/4 is intended to provide data to assess fission product retention and transport (e.g., diffusion coefficients) in fuel matrix and graphite materials. We describe a set of pre-test predictions that incorporate the results of detailed thermal and fission product release models into a coupled 1D radial diffusion model of the experiment, using diffusion coefficients reported in the literature for Ag, Cs, and Sr. We make some comparisons of the predicted Cs profiles to preliminary measured data for Cs and find these to bemore » reasonable, in most cases within an order of magnitude. Our ultimate objective is to refine the diffusion coefficients using AGR-3/4 data, so we identify an analytical method for doing so and demonstrate its efficacy via a series of numerical experiments using the model predictions. Finally, we discuss development of a post-irradiation examination plan informed by the modeling effort and simulate some of the heating tests that are tentatively planned.« less

14 CFR 121.453 - Flight engineer qualifications.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Flight engineer qualifications. 121.453... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Crewmember Qualifications § 121.453 Flight engineer qualifications. (a) No certificate holder may use any person nor may any person serve as a flight engineer on an...

14 CFR 121.453 - Flight engineer qualifications.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Flight engineer qualifications. 121.453... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Crewmember Qualifications § 121.453 Flight engineer qualifications. (a) No certificate holder may use any person nor may any person serve as a flight engineer on an...

14 CFR 121.453 - Flight engineer qualifications.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Flight engineer qualifications. 121.453... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Crewmember Qualifications § 121.453 Flight engineer qualifications. (a) No certificate holder may use any person nor may any person serve as a flight engineer on an...

14 CFR 121.453 - Flight engineer qualifications.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Flight engineer qualifications. 121.453... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Crewmember Qualifications § 121.453 Flight engineer qualifications. (a) No certificate holder may use any person nor may any person serve as a flight engineer on an...

14 CFR 121.453 - Flight engineer qualifications.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Flight engineer qualifications. 121.453... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Crewmember Qualifications § 121.453 Flight engineer qualifications. (a) No certificate holder may use any person nor may any person serve as a flight engineer on an...

29 CFR 1926.1428 - Signal person qualifications.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 8 2012-07-01 2012-07-01 false Signal person qualifications. 1926.1428 Section 1926.1428... Construction § 1926.1428 Signal person qualifications. (a) The employer of the signal person must ensure that each signal person meets the Qualification Requirements (paragraph (c) of this section) prior to giving...

29 CFR 1926.1428 - Signal person qualifications.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 8 2014-07-01 2014-07-01 false Signal person qualifications. 1926.1428 Section 1926.1428... Construction § 1926.1428 Signal person qualifications. (a) The employer of the signal person must ensure that each signal person meets the Qualification Requirements (paragraph (c) of this section) prior to giving...

29 CFR 1926.1428 - Signal person qualifications.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 8 2013-07-01 2013-07-01 false Signal person qualifications. 1926.1428 Section 1926.1428... Construction § 1926.1428 Signal person qualifications. (a) The employer of the signal person must ensure that each signal person meets the Qualification Requirements (paragraph (c) of this section) prior to giving...

5 CFR 339.205 - Medical evaluation programs.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 5 Administrative Personnel 1 2013-01-01 2013-01-01 false Medical evaluation programs. 339.205 Section 339.205 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS MEDICAL QUALIFICATION DETERMINATIONS Physical and Medical Qualifications § 339.205 Medical evaluation programs. Agencies...

5 CFR 339.205 - Medical evaluation programs.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 5 Administrative Personnel 1 2014-01-01 2014-01-01 false Medical evaluation programs. 339.205 Section 339.205 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS MEDICAL QUALIFICATION DETERMINATIONS Physical and Medical Qualifications § 339.205 Medical evaluation programs. Agencies...

5 CFR 339.205 - Medical evaluation programs.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 5 Administrative Personnel 1 2011-01-01 2011-01-01 false Medical evaluation programs. 339.205 Section 339.205 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS MEDICAL QUALIFICATION DETERMINATIONS Physical and Medical Qualifications § 339.205 Medical evaluation programs. Agencies...

Qualities and Qualifications of EFL Professionals: What Do Intensive English Program Administrators Think?

ERIC Educational Resources Information Center

Akcan, Sumru; Aydin, Belgin; Karaman, A. Cendel; Seferoglu, Gölge; Korkmazgil, Sibel; Özbilgin, Alev; Selvi, Ali Fuad

2017-01-01

Recent educational research has underscored the criticality and centrality of teacher quality as a decisive and prominent influence on students' academic growth and success. Consequently, it becomes imperative to define, understand, and study the qualities and qualifications of effective teachers so as to inform preservice and in-service teacher…

[Introduction and analysis on acupuncture education and qualification examination system in U.S.A].

PubMed

Lu, Cong; He, Wei; Zhao, Ying-Kai

2013-12-01

Based on research of the Accreditation Commission for Acupuncture and Oriental Medicine (ACAOM), the National Certification Commission for Acupuncture and Oriental Medicine (NCCAOM) and the related public websites of acupuncture educational institutions, the first hand information was studied, summarized and sorted on the status quo of the educational qualification or the relative authentication of education programs of ACAOM as well as the status of professional qualification and examination of NCCAOM. The education curriculum of the Master degree of Acupuncture, Master degree of Oriental Medicine Science as well as the Doctor degree of Acupuncture and Oriental Medicine were also introduced in this article including student enrollment and graduation in recent years. The developing tendency of the federal unified acupuncture education, management, professional qualification and examination system of U. S. A. were discussed and analyzed as well.

13kW Advanced Electric Propulsion Flight System Development and Qualification

NASA Technical Reports Server (NTRS)

Jackson, Jerry; Allen, May; Myers, Roger; Soendker, Erich; Welander, Benjamin; Tolentino, Artie; Hablitzel, Sam; Yeatts, Chyrl; Xu, Steven; Sheehan, Chris;

32 CFR 171.4 - Qualifications.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 1 2014-07-01 2014-07-01 false Qualifications. 171.4 Section 171.4 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DEFENSE CONTRACTING IMPLEMENTATION OF WILDFIRE SUPPRESSION AIRCRAFT TRANSFER ACT OF 1996 § 171.4 Qualifications. The Secretary of Agriculture...

32 CFR 171.4 - Qualifications.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 1 2013-07-01 2013-07-01 false Qualifications. 171.4 Section 171.4 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DEFENSE CONTRACTING IMPLEMENTATION OF WILDFIRE SUPPRESSION AIRCRAFT TRANSFER ACT OF 1996 § 171.4 Qualifications. The Secretary of Agriculture...

32 CFR 171.4 - Qualifications.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 1 2012-07-01 2012-07-01 false Qualifications. 171.4 Section 171.4 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DEFENSE CONTRACTING IMPLEMENTATION OF WILDFIRE SUPPRESSION AIRCRAFT TRANSFER ACT OF 1996 § 171.4 Qualifications. The Secretary of Agriculture...

32 CFR 171.4 - Qualifications.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 1 2011-07-01 2011-07-01 false Qualifications. 171.4 Section 171.4 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DEFENSE CONTRACTING IMPLEMENTATION OF WILDFIRE SUPPRESSION AIRCRAFT TRANSFER ACT OF 1996 § 171.4 Qualifications. The Secretary of Agriculture...

42 CFR 61.5 - Qualifications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 1 2013-10-01 2013-10-01 false Qualifications. 61.5 Section 61.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Regular Fellowships § 61.5 Qualifications. In order to qualify for a regular fellowship, an...

42 CFR 61.5 - Qualifications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false Qualifications. 61.5 Section 61.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Regular Fellowships § 61.5 Qualifications. In order to qualify for a regular fellowship, an...

42 CFR 61.5 - Qualifications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 1 2012-10-01 2012-10-01 false Qualifications. 61.5 Section 61.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Regular Fellowships § 61.5 Qualifications. In order to qualify for a regular fellowship, an...

42 CFR 61.5 - Qualifications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 1 2011-10-01 2011-10-01 false Qualifications. 61.5 Section 61.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Regular Fellowships § 61.5 Qualifications. In order to qualify for a regular fellowship, an...

42 CFR 61.5 - Qualifications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 1 2014-10-01 2014-10-01 false Qualifications. 61.5 Section 61.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Regular Fellowships § 61.5 Qualifications. In order to qualify for a regular fellowship, an...

78 FR 67799 - Qualification, Service, and Use of Crewmembers and Aircraft Dispatchers

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-12

...This final rule revises the training requirements for pilots in air carrier operations. The regulations enhance air carrier pilot training programs by emphasizing the development of pilots' manual handling skills and adding safety-critical tasks such as recovery from stall and upset. The final rule also requires enhanced runway safety training and pilot monitoring training to be incorporated into existing requirements for scenario-based flight training and requires air carriers to implement remedial training programs for pilots. The FAA expects these changes to contribute to a reduction in aviation accidents. Additionally, the final rule revises recordkeeping requirements for communications between the flightcrew and dispatch; ensures that personnel identified as flight attendants have completed flight attendant training and qualification requirements; provides civil enforcement authority for making fraudulent statements; and, provides a number of conforming and technical changes to existing air carrier crewmember training and qualification requirements. The final rule also includes provisions that provide opportunities for air carriers to modify training program requirements for flightcrew members when the air carrier operates multiple aircraft types with similar design and flight handling characteristics.

10 CFR 1042.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Sex as a bona fide occupational qualification. 1042.550 Section 1042.550 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex...

10 CFR 5.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 1 2014-01-01 2014-01-01 false Sex as a bona fide occupational qualification. 5.550 Section 5.550 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

10 CFR 5.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 1 2011-01-01 2011-01-01 false Sex as a bona fide occupational qualification. 5.550 Section 5.550 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

10 CFR 1042.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Sex as a bona fide occupational qualification. 1042.550 Section 1042.550 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex...

10 CFR 5.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Sex as a bona fide occupational qualification. 5.550 Section 5.550 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

10 CFR 1042.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Sex as a bona fide occupational qualification. 1042.550 Section 1042.550 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex...

10 CFR 5.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 1 2012-01-01 2012-01-01 false Sex as a bona fide occupational qualification. 5.550 Section 5.550 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

10 CFR 1042.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Sex as a bona fide occupational qualification. 1042.550 Section 1042.550 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex...

10 CFR 1042.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Sex as a bona fide occupational qualification. 1042.550 Section 1042.550 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex...

10 CFR 5.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 1 2013-01-01 2013-01-01 false Sex as a bona fide occupational qualification. 5.550 Section 5.550 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

Space qualification of silicon carbide for mirror applications: progress and future objectives

NASA Astrophysics Data System (ADS)

Palusinski, Iwona A.; Ghozeil, Isaac

2006-09-01

Production of optical silicon carbide (SiC) for mirror applications continues to evolve and there are renewed plans to use this material in future space-based systems. While SiC has the potential for rapid and cost-effective manufacturing of large, lightweight, athermal optical systems, this material's use in mirror applications is relatively new and has limited flight heritage. This combination of drivers stresses the necessity for a space qualification program for this material. Successful space qualification will require independent collaboration to absorb the high cost of executing this program while taking advantage of each contributing group's laboratory expertise to develop a comprehensive SiC database. This paper provides an overview of the trends and progress in the production of SiC, and identifies future objectives such as non-destructive evaluation and space-effects modeling to ensure proper implementation of this material into future space-based systems.

46 CFR 56.70-20 - Qualification, general.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Qualification, general. 56.70-20 Section 56.70-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Fabrication, Assembly and Erection § 56.70-20 Qualification, general. (a) Qualification of the welding procedures to be used, and of the...

46 CFR 56.70-20 - Qualification, general.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Qualification, general. 56.70-20 Section 56.70-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Fabrication, Assembly and Erection § 56.70-20 Qualification, general. (a) Qualification of the welding procedures to be used, and of the...

46 CFR 56.75-20 - Brazing qualification.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Brazing qualification. 56.75-20 Section 56.75-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-20 Brazing qualification. (a) The qualification of the performance of brazers and brazing operators shall be in accordance...

46 CFR 56.70-20 - Qualification, general.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Qualification, general. 56.70-20 Section 56.70-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Fabrication, Assembly and Erection § 56.70-20 Qualification, general. (a) Qualification of the welding procedures to be used, and of the...

46 CFR 56.70-20 - Qualification, general.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Qualification, general. 56.70-20 Section 56.70-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Fabrication, Assembly and Erection § 56.70-20 Qualification, general. (a) Qualification of the welding procedures to be used, and of the...

46 CFR 56.70-20 - Qualification, general.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Qualification, general. 56.70-20 Section 56.70-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Fabrication, Assembly and Erection § 56.70-20 Qualification, general. (a) Qualification of the welding procedures to be used, and of the...

46 CFR 56.75-20 - Brazing qualification.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Brazing qualification. 56.75-20 Section 56.75-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-20 Brazing qualification. (a) The qualification of the performance of brazers and brazing operators shall be in accordance...

46 CFR 56.75-20 - Brazing qualification.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Brazing qualification. 56.75-20 Section 56.75-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-20 Brazing qualification. (a) The qualification of the performance of brazers and brazing operators shall be in accordance...

46 CFR 56.75-20 - Brazing qualification.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Brazing qualification. 56.75-20 Section 56.75-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-20 Brazing qualification. (a) The qualification of the performance of brazers and brazing operators shall be in accordance...

46 CFR 56.75-20 - Brazing qualification.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Brazing qualification. 56.75-20 Section 56.75-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-20 Brazing qualification. (a) The qualification of the performance of brazers and brazing operators shall be in accordance...

Unintended Consequences: How Qualification Constrains Innovation

NASA Technical Reports Server (NTRS)

Brice, Craig A.

2011-01-01

The development and implementation of new materials and manufacturing processes for aerospace application is often hindered by the high cost and long time span associated with current qualification procedures. The data requirements necessary for material and process qualification are extensive and often require millions of dollars and multiple years to complete. Furthermore, these qualification data can become obsolete for even minor changes to the processing route. This burden is a serious impediment to the pursuit of revolutionary new materials and more affordable processing methods for air vehicle structures. The application of integrated computational materials engineering methods to this problem can help to reduce the barriers to rapid insertion of new materials and processes. By establishing predictive capability for the development of microstructural features in relation to processing and relating this to critical property characteristics, a streamlined approach to qualification is possible. This paper critically examines the advantages and challenges to a modeling-assisted qualification approach for aerospace structural materials. An example of how this approach might apply towards the emerging field of additive manufacturing is discussed in detail.

School Mathematics Qualifications and Work

ERIC Educational Resources Information Center

Drake, Pat; Noyes, Andrew; Wake, Geoff

2012-01-01

This article explores the relationship between school mathematics qualifications and the transition into employment. As part of a large-scale, three-year independent evaluation of pilot qualifications in 14-19 school mathematics, all 39 Sector Skills Councils and Employment Skills Boards in the UK were invited to provide evidence regarding their…

Space Qualification Test of a-Silicon Solar Cell Modules

NASA Technical Reports Server (NTRS)

Kim, Q.; Lawton, R. A.; Manion, S. J.; Okuno, J. O.; Ruiz, R. P.; Vu, D. T.; Kayali, S. A.; Jeffrey, F. R.

1997-01-01

The basic requirement of solar cell modules for space applications are generally described in MIL-S-83576 for the specific needs of the USAF. However, the specifications of solar cells intended for use on space terrestrial applications are not well defined. Therefore this qualification test effort was concentrated on critical areas specific to the microseismometer probe which is intended to be included in the Mars microprobe programs.

29 CFR 36.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 1 2012-07-01 2012-07-01 false Sex as a bona fide occupational qualification. 36.550 Section 36.550 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

29 CFR 36.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 1 2011-07-01 2011-07-01 false Sex as a bona fide occupational qualification. 36.550 Section 36.550 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

29 CFR 36.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 1 2013-07-01 2013-07-01 false Sex as a bona fide occupational qualification. 36.550 Section 36.550 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

29 CFR 36.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 1 2014-07-01 2013-07-01 true Sex as a bona fide occupational qualification. 36.550 Section 36.550 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

29 CFR 36.550 - Sex as a bona fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 1 2010-07-01 2010-07-01 true Sex as a bona fide occupational qualification. 36.550 Section 36.550 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

Processing and microstructural characterisation of a UO2-based ceramic for disposal studies on spent AGR fuel

NASA Astrophysics Data System (ADS)

Hiezl, Z.; Hambley, D. I.; Padovani, C.; Lee, W. E.

2015-01-01

Preparation and characterisation of a Simulated Spent Nuclear Fuel (SIMFuel), which replicates the chemical state and microstructure of Spent Nuclear Fuel (SNF) discharged from a UK Advanced Gas-cooled Reactor (AGR) after a cooling time of 100 years is described. Given the relatively small differences in radionuclide inventory expected over longer time periods, the SIMFuel studied in this work is expected to be also representative of spent fuel after significantly longer periods (e.g. 1000 years). Thirteen stable elements were added to depleted UO2 and sintered to simulate the composition of fuel pellets after burn-ups of 25 and 43 GWd/tU and, as a reference, pure UO2 pellets were also investigated. The fission product distribution was calculated using the FISPIN code provided by the UK National Nuclear Laboratory. SIMFuel pellets were up to 92% dense and during the sintering process in H2 atmosphere Mo-Ru-Rh-Pd metallic precipitates and grey-phase ((Ba, Sr)(Zr, RE) O3 oxide precipitates) formed within the UO2 matrix. These secondary phases are present in real PWR and AGR SNF. Metallic precipitates are generally spherical and have submicron particle size (0.8 ± 0.7 μm). Spherical oxide precipitates in SIMFuel measured up to 30 μm in diameter, but no data were available in the public domain to compare this to AGR SNF. The grain size of actual AGR SNF (∼ 3-30 μm) is larger than that measured in AGR SIMFuel (∼ 2-5 μm).

From Old to New: The Australian Qualifications Framework

ERIC Educational Resources Information Center

Wheelahan, Leesa

2011-01-01

The Australian Qualifications Framework (AQF) is a "first generation" qualifications framework that was established in 1995. Its purpose was to create "a comprehensive, nationally consistent yet flexible framework for all qualifications in post-compulsory education and training." It encompasses all post-compulsory…

14 CFR 121.412 - Qualifications: Flight instructors (airplane) and flight instructors (simulator).

Code of Federal Regulations, 2010 CFR

2010-01-01

... (airplane) and flight instructors (simulator). 121.412 Section 121.412 Aeronautics and Space FEDERAL... OPERATIONS Training Program § 121.412 Qualifications: Flight instructors (airplane) and flight instructors (simulator). (a) For the purposes of this section and § 121.414: (1) A flight instructor (airplane) is a...

14 CFR 121.412 - Qualifications: Flight instructors (airplane) and flight instructors (simulator).

Code of Federal Regulations, 2014 CFR

2014-01-01

... (airplane) and flight instructors (simulator). 121.412 Section 121.412 Aeronautics and Space FEDERAL... OPERATIONS Training Program § 121.412 Qualifications: Flight instructors (airplane) and flight instructors... section and § 121.414: (1) A flight instructor (airplane) is a person who is qualified to instruct in an...

14 CFR 121.412 - Qualifications: Flight instructors (airplane) and flight instructors (simulator).

Code of Federal Regulations, 2012 CFR

2012-01-01

... (airplane) and flight instructors (simulator). 121.412 Section 121.412 Aeronautics and Space FEDERAL... OPERATIONS Training Program § 121.412 Qualifications: Flight instructors (airplane) and flight instructors (simulator). (a) For the purposes of this section and § 121.414: (1) A flight instructor (airplane) is a...

14 CFR 121.412 - Qualifications: Flight instructors (airplane) and flight instructors (simulator).

Code of Federal Regulations, 2013 CFR

2013-01-01

... (airplane) and flight instructors (simulator). 121.412 Section 121.412 Aeronautics and Space FEDERAL... OPERATIONS Training Program § 121.412 Qualifications: Flight instructors (airplane) and flight instructors (simulator). (a) For the purposes of this section and § 121.414: (1) A flight instructor (airplane) is a...

14 CFR 121.412 - Qualifications: Flight instructors (airplane) and flight instructors (simulator).

Code of Federal Regulations, 2011 CFR

2011-01-01

... (airplane) and flight instructors (simulator). 121.412 Section 121.412 Aeronautics and Space FEDERAL... OPERATIONS Training Program § 121.412 Qualifications: Flight instructors (airplane) and flight instructors (simulator). (a) For the purposes of this section and § 121.414: (1) A flight instructor (airplane) is a...

76 FR 45271 - Review and Qualification of Clinical Outcome Assessments; Public Workshop

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-28

... announcing a public workshop to discuss measurement principles for clinical outcome assessments (COAs) for... appropriate drug development program. Because the qualification process is separate from the drug marketing... other DDTs. This workshop will focus on FDA review principles specific to all type of COAs, i.e., PRO...

40 CFR 60.3014 - What are the operator training and qualification requirements?

Code of Federal Regulations, 2010 CFR

2010-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Emission Guidelines... and qualification requirements? (a) No OSWI unit can be operated unless a fully trained and qualified... trained and qualified OSWI unit operator may operate the OSWI unit directly or be the direct supervisor of...

40 CFR 60.3014 - What are the operator training and qualification requirements?

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Emission Guidelines... and qualification requirements? (a) No OSWI unit can be operated unless a fully trained and qualified... trained and qualified OSWI unit operator may operate the OSWI unit directly or be the direct supervisor of...

40 CFR 60.3014 - What are the operator training and qualification requirements?

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Emission Guidelines... and qualification requirements? (a) No OSWI unit can be operated unless a fully trained and qualified... trained and qualified OSWI unit operator may operate the OSWI unit directly or be the direct supervisor of...

40 CFR 60.3014 - What are the operator training and qualification requirements?

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Emission Guidelines... and qualification requirements? (a) No OSWI unit can be operated unless a fully trained and qualified... trained and qualified OSWI unit operator may operate the OSWI unit directly or be the direct supervisor of...

40 CFR 60.3014 - What are the operator training and qualification requirements?

Code of Federal Regulations, 2011 CFR

2011-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Emission Guidelines... and qualification requirements? (a) No OSWI unit can be operated unless a fully trained and qualified... trained and qualified OSWI unit operator may operate the OSWI unit directly or be the direct supervisor of...

48 CFR 1553.209 - Contractor qualifications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contractor qualifications. 1553.209 Section 1553.209 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION AGENCY CLAUSES AND FORMS FORMS Prescription of Forms 1553.209 Contractor qualifications. ...

Qualifications Mismatch and Skills Mismatch

ERIC Educational Resources Information Center

Sutherland, John

2012-01-01

Purpose: The purpose of this paper is to estimate the extent of "over-qualification" (i.e. holding a qualification which is above that required to gain entry to the job being done) and "skills under-utilisation" (i.e. being in a job which does not make use of the knowledge and skills possessed) in the United Kingdom and to…

28 CFR 34.109 - Qualifications of peer reviewers.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Section 34.109 Judicial Administration DEPARTMENT OF JUSTICE OJJDP COMPETITION AND PEER REVIEW PROCEDURES Peer Review § 34.109 Qualifications of peer reviewers. The general reviewer qualification criteria to...). Additional details concerning peer reviewer qualifications are provided in the OJJDP “Peer Review Guideline”. ...

28 CFR 50.24 - Annuity broker minimum qualifications.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Annuity broker minimum qualifications. 50....24 Annuity broker minimum qualifications. (a) Minimum standards. The Civil Division, United States Department of Justice, shall establish a list of annuity brokers who meet minimum qualifications for...

5 CFR 317.502 - Qualifications Review Board certification.

Code of Federal Regulations, 2010 CFR

2010-01-01

... REGULATIONS EMPLOYMENT IN THE SENIOR EXECUTIVE SERVICE Career Appointments § 317.502 Qualifications Review Board certification. (a) A Qualification Review Board (QRB) convened by OPM must certify the executive... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Qualifications Review Board certification...

48 CFR 53.209 - Contractor qualifications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Contractor qualifications. 53.209 Section 53.209 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS FORMS Prescription of Forms 53.209 Contractor qualifications. ...

14 CFR 121.439 - Pilot qualification: Recent experience.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Pilot qualification: Recent experience. 121... Pilot qualification: Recent experience. Link to an amendment published at 78 FR 67841, Nov. 12, 2013. (a... qualification: Recent experience. (f) Deviation authority based upon designation of related aircraft in...

Microscopic analysis of irradiated AGR-1 coated particle fuel compacts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Scott A. Ploger; Paul A. Demkowicz; John D. Hunn

The AGR-1 experiment involved irradiation of 72 TRISO-coated particle fuel compacts to a peak compact-average burnup of 19.5% FIMA with no in-pile failures observed out of 3 x 105 total particles. Irradiated AGR-1 fuel compacts have been cross-sectioned and analyzed with optical microscopy to characterize kernel, buffer, and coating behavior. Six compacts have been examined, spanning a range of irradiation conditions (burnup, fast fluence, and irradiation temperature) and including all four TRISO coating variations irradiated in the AGR-1 experiment. The cylindrical specimens were sectioned both transversely and longitudinally, then polished to expose from 36 to 79 individual particles near midplanemore » on each mount. The analysis focused primarily on kernel swelling and porosity, buffer densification and fracturing, buffer–IPyC debonding, and fractures in the IPyC and SiC layers. Characteristic morphologies have been identified, 981 particles have been classified, and spatial distributions of particle types have been mapped. No significant spatial patterns were discovered in these cross sections. However, some trends were found between morphological types and certain behavioral aspects. Buffer fractures were found in 23% of the particles, and these fractures often resulted in unconstrained kernel protrusion into the open cavities. Fractured buffers and buffers that stayed bonded to IPyC layers appear related to larger pore size in kernels. Buffer–IPyC interface integrity evidently factored into initiation of rare IPyC fractures. Fractures through part of the SiC layer were found in only four classified particles, all in conjunction with IPyC–SiC debonding. Compiled results suggest that the deliberate coating fabrication variations influenced the frequencies of IPyC fractures and IPyC–SiC debonds.« less

Reliability Evaluation of Concentrator Photovoltaic Modules per IEC Qualification Specifications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tamizhmani, Govindasamy

2012-12-05

This project is related to the qualification testing of new generation CPV (concentrator photovoltaics) modules at lower testing costs and lower turnaround time. In this project, the first testing program was completed for two CPV manufacturers, the second testing program was completed for two manufacturers at 65% of the actual testing cost and at less than 3 months of testing turnaround time and the third testing program was completed for two manufacturers at 65% of the actual testing cost and at less than 3 months of testing turnaround time. Due to their financial situation and restructuring, Amonix (one of themore » CPV manufacturers) intermittently terminated the test programs.« less

Complex regulation of Arabidopsis AGR1/PIN2-mediated root gravitropic response and basipetal auxin transport by cantharidin-sensitive protein phosphatases

NASA Technical Reports Server (NTRS)

Shin, Heungsop; Shin, Hwa-Soo; Guo, Zibiao; Blancaflor, Elison B.; Masson, Patrick H.; Chen, Rujin

2005-01-01

Polar auxin transport, mediated by two distinct plasma membrane-localized auxin influx and efflux carrier proteins/complexes, plays an important role in many plant growth and developmental processes including tropic responses to gravity and light, development of lateral roots and patterning in embryogenesis. We have previously shown that the Arabidopsis AGRAVITROPIC 1/PIN2 gene encodes an auxin efflux component regulating root gravitropism and basipetal auxin transport. However, the regulatory mechanism underlying the function of AGR1/PIN2 is largely unknown. Recently, protein phosphorylation and dephosphorylation mediated by protein kinases and phosphatases, respectively, have been implicated in regulating polar auxin transport and root gravitropism. Here, we examined the effects of chemical inhibitors of protein phosphatases on root gravitropism and basipetal auxin transport, as well as the expression pattern of AGR1/PIN2 gene and the localization of AGR1/PIN2 protein. We also examined the effects of inhibitors of vesicle trafficking and protein kinases. Our data suggest that protein phosphatases, sensitive to cantharidin and okadaic acid, are likely involved in regulating AGR1/PIN2-mediated root basipetal auxin transport and gravitropism, as well as auxin response in the root central elongation zone (CEZ). BFA-sensitive vesicle trafficking may be required for the cycling of AGR1/PIN2 between plasma membrane and the BFA compartment, but not for the AGR1/PIN2-mediated root basipetal auxin transport and auxin response in CEZ cells.

40 CFR 60.4810 - What are the operator training and qualification requirements?

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Standards of... the operator training and qualification requirements? (a) A SSI unit cannot be operated unless a fully... within 1 hour. The trained and qualified SSI unit operator may operate the SSI unit directly or be the...

40 CFR 60.4810 - What are the operator training and qualification requirements?

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Standards of... the operator training and qualification requirements? (a) A SSI unit cannot be operated unless a fully... within 1 hour. The trained and qualified SSI unit operator may operate the SSI unit directly or be the...

40 CFR 60.4810 - What are the operator training and qualification requirements?

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Standards of... the operator training and qualification requirements? (a) A SSI unit cannot be operated unless a fully... within 1 hour. The trained and qualified SSI unit operator may operate the SSI unit directly or be the...

49 CFR 180.603 - Qualification of portable tanks.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Qualification of portable tanks. 180.603 Section... MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Portable Tanks § 180.603 Qualification of portable tanks. (a) Each portable tank used for the transportation of hazardous materials must be an authorized...

Unit Standards Catalogue. Unit Standards and Qualifications Registered on the National Qualifications Framework to March 1994.

ERIC Educational Resources Information Center

New Zealand Qualifications Authority, Wellington.

This booklet includes the latest list of unit standards and qualifications registered on the New Zealand National Qualifications Framework to April 1994. Unit standards registered on the framework can be offered by private and government training establishments, polytechnics, colleges of education, and schools. This list of registered unit…

5 CFR 319.301 - Qualifications standards.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 5 Administrative Personnel 1 2011-01-01 2011-01-01 false Qualifications standards. 319.301 Section... IN SENIOR-LEVEL AND SCIENTIFIC AND PROFESSIONAL POSITIONS Qualifications Requirements § 319.301... in the General Schedule. (c) Standards for scientific and professional positions. (1) Unless the...

5 CFR 319.301 - Qualifications standards.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Qualifications standards. 319.301 Section... IN SENIOR-LEVEL AND SCIENTIFIC AND PROFESSIONAL POSITIONS Qualifications Requirements § 319.301... in the General Schedule. (c) Standards for scientific and professional positions. (1) Unless the...

16 CFR 1210.14 - Qualification testing.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Qualification testing. 1210.14 Section 1210.14 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS SAFETY STANDARD FOR CIGARETTE LIGHTERS Certification Requirements § 1210.14 Qualification testing. (a...

16 CFR 1209.34 - Qualification testing.

Code of Federal Regulations, 2010 CFR

2010-01-01

....34 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS INTERIM SAFETY STANDARD FOR CELLULOSE INSULATION Certification § 1209.34 Qualification testing. (a... labelers, or importers may select samples for qualification testing of a product in any manner they desire. ...

5 CFR 330.709 - Qualification reviews.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Qualification reviews. 330.709 Section..., SELECTION, AND PLACEMENT (GENERAL) Interagency Career Transition Assistance Plan for Displaced Employees § 330.709 Qualification reviews. Agencies will ensure that a documented, independent second review is...

48 CFR 253.209 - Contractor qualifications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Contractor qualifications. 253.209 Section 253.209 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS FORMS Prescription of Forms 253.209 Contractor qualifications. ...

Qualification Procedures for VHSIC/VLSI

DTIC Science & Technology

1990-12-01

alternative approach for qualification of complex microcircuits. To address the technical issues related to a process oriented qualification approach, the...methodology of microcircuit process control to promote the United States to a position of supplying the nighest quality and most reliable...available resources . o Coordinate document reviews with weekly and monthly status reviews on progress. o Summarize results and collate into four basic

Identification of a Hydrophobic Cleft in the LytTR Domain of AgrA as a Locus for Small Molecule Interactions that Inhibit DNA Binding

PubMed Central

Leonard, Paul G.; Bezar, Ian F.; Sidote, David J.; Stock, Ann M.

2012-01-01

The AgrA transcription factor regulates the quorum-sensing response in Staphylococcus aureus, controlling the production of hemolysins and other virulence factors. AgrA binds to DNA via its C-terminal LytTR domain, a domain not found in humans but common in many pathogenic bacteria, making it a potential target for antimicrobial development. We have determined the crystal structure of the apo AgrA LytTR domain and screened a library of 500 fragment compounds to find inhibitors of AgrA DNA-binding activity. Using NMR, the binding site for five compounds has been mapped to a common locus at the C-terminal end of the LytTR domain, a site known to be important for DNA-binding activity. Three of these compounds inhibit AgrA DNA binding. These results provide the first evidence that LytTR domains can be targeted by small organic compounds. PMID:23181972

28 CFR 115.402 - Auditor qualifications.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Auditor qualifications. 115.402 Section 115.402 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Auditing and Corrective Action § 115.402 Auditor qualifications. (a) An audit shall be conducted...

28 CFR 115.402 - Auditor qualifications.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Auditor qualifications. 115.402 Section 115.402 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Auditing and Corrective Action § 115.402 Auditor qualifications. (a) An audit shall be conducted...

28 CFR 115.402 - Auditor qualifications.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Auditor qualifications. 115.402 Section 115.402 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Auditing and Corrective Action § 115.402 Auditor qualifications. (a) An audit shall be conducted...

5 CFR 330.609 - Qualification reviews.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Qualification reviews. 330.609 Section..., SELECTION, AND PLACEMENT (GENERAL) Agency Career Transition Assistance Plans (CTAP) for Local Surplus and Displaced Employees § 330.609 Qualification reviews. Agencies will ensure that a documented, independent...

7 CFR 15a.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 1 2010-01-01 2010-01-01 false Sex as a bona-fide occupational qualification. 15a.61... RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs and Activities Prohibited § 15a.61 Sex as a bona-fide occupational...

7 CFR 15a.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 1 2014-01-01 2014-01-01 false Sex as a bona-fide occupational qualification. 15a.61... RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs and Activities Prohibited § 15a.61 Sex as a bona-fide occupational...

7 CFR 15a.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 1 2012-01-01 2012-01-01 false Sex as a bona-fide occupational qualification. 15a.61... RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs and Activities Prohibited § 15a.61 Sex as a bona-fide occupational...

7 CFR 15a.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 1 2011-01-01 2011-01-01 false Sex as a bona-fide occupational qualification. 15a.61... RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs and Activities Prohibited § 15a.61 Sex as a bona-fide occupational...

7 CFR 15a.61 - Sex as a bona-fide occupational qualification.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 1 2013-01-01 2013-01-01 false Sex as a bona-fide occupational qualification. 15a.61... RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs and Activities Prohibited § 15a.61 Sex as a bona-fide occupational...

49 CFR 180.507 - Qualification of tank cars.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Qualification of tank cars. 180.507 Section 180... MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.507 Qualification of tank cars. (a) Each tank car marked as meeting a “DOT” specification or any other tank car used for the transportation...

49 CFR 180.507 - Qualification of tank cars.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 3 2014-10-01 2014-10-01 false Qualification of tank cars. 180.507 Section 180... MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.507 Qualification of tank cars. (a) Each tank car marked as meeting a “DOT” specification or any other tank car used for the transportation...

49 CFR 180.507 - Qualification of tank cars.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 3 2011-10-01 2011-10-01 false Qualification of tank cars. 180.507 Section 180... MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.507 Qualification of tank cars. (a) Each tank car marked as meeting a “DOT” specification or any other tank car used for the transportation...

49 CFR 180.507 - Qualification of tank cars.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 3 2012-10-01 2012-10-01 false Qualification of tank cars. 180.507 Section 180... MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.507 Qualification of tank cars. (a) Each tank car marked as meeting a “DOT” specification or any other tank car used for the transportation...

46 CFR 57.05-3 - Limited space qualifications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Limited space qualifications. 57.05-3 Section 57.05-3... Performance Qualifications § 57.05-3 Limited space qualifications. When a welder is to be qualified for welding or torch brazing of piping on board ship in a limited or restricted space, the space restrictions...

46 CFR 57.05-3 - Limited space qualifications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Limited space qualifications. 57.05-3 Section 57.05-3... Performance Qualifications § 57.05-3 Limited space qualifications. When a welder is to be qualified for welding or torch brazing of piping on board ship in a limited or restricted space, the space restrictions...

46 CFR 57.05-3 - Limited space qualifications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Limited space qualifications. 57.05-3 Section 57.05-3... Performance Qualifications § 57.05-3 Limited space qualifications. When a welder is to be qualified for welding or torch brazing of piping on board ship in a limited or restricted space, the space restrictions...

46 CFR 57.05-3 - Limited space qualifications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Limited space qualifications. 57.05-3 Section 57.05-3... Performance Qualifications § 57.05-3 Limited space qualifications. When a welder is to be qualified for welding or torch brazing of piping on board ship in a limited or restricted space, the space restrictions...

21 CFR 211.25 - Personnel qualifications.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 4 2014-04-01 2014-04-01 false Personnel qualifications. 211.25 Section 211.25 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS....25 Personnel qualifications. (a) Each person engaged in the manufacture, processing, packing, or...

21 CFR 211.25 - Personnel qualifications.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 4 2013-04-01 2013-04-01 false Personnel qualifications. 211.25 Section 211.25 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS....25 Personnel qualifications. (a) Each person engaged in the manufacture, processing, packing, or...

21 CFR 211.25 - Personnel qualifications.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 4 2012-04-01 2012-04-01 false Personnel qualifications. 211.25 Section 211.25 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS....25 Personnel qualifications. (a) Each person engaged in the manufacture, processing, packing, or...

34 CFR 300.156 - Personnel qualifications.

Code of Federal Regulations, 2010 CFR

2010-07-01

... children with disabilities. (c) Qualifications for special education teachers. The qualifications described in paragraph (a) of this section must ensure that each person employed as a public school special education teacher in the State who teaches in an elementary school, middle school, or secondary school is...

9 CFR 2.32 - Personnel qualifications.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Personnel qualifications. 2.32 Section 2.32 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Research Facilities § 2.32 Personnel qualifications. (a) It shall be...

9 CFR 2.32 - Personnel qualifications.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Personnel qualifications. 2.32 Section 2.32 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Research Facilities § 2.32 Personnel qualifications. (a) It shall be...

9 CFR 2.32 - Personnel qualifications.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Personnel qualifications. 2.32 Section 2.32 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Research Facilities § 2.32 Personnel qualifications. (a) It shall be...

9 CFR 2.32 - Personnel qualifications.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Personnel qualifications. 2.32 Section 2.32 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Research Facilities § 2.32 Personnel qualifications. (a) It shall be...

9 CFR 2.32 - Personnel qualifications.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Personnel qualifications. 2.32 Section 2.32 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Research Facilities § 2.32 Personnel qualifications. (a) It shall be...

Guide for the Training and Qualification of Welding Personnel. Level III - Expert Welders.

ERIC Educational Resources Information Center

American Welding Society, Miami, FL.

This guide is designed to help education and training facilities develop and administer competency-based training programs to qualify and certify trainees in accordance with American Welding Society (AWS) requirements for level III (expert) welders. Presented first are the scope/objectives/requirements of the AWS qualification/certification…

Qualification Status of Non-Asbestos Internal Insulation in the Reusable Solid Rocket Motor Program

NASA Technical Reports Server (NTRS)

Clayton, Louie

2011-01-01

This paper provides a status of the qualification efforts associated with NASA's RSRMV non-asbestos internal insulation program. For many years, NASA has been actively engaged in removal of asbestos from the shuttle RSRM motors due to occupation health concerns where technicians are working with an EPA banned material. Careful laboratory and subscale testing has lead to the downselect of a organic fiber known as Polybenzimidazol to replace the asbestos fiber filler in the existing synthetic rubber copolymer Nitrile Butadiene - now named PBI/NBR. Manufacturing, processing, and layup of the new material has been a challenge due to the differences in the baseline shuttle RSRM internal insulator properties and PBI/NBR material properties. For this study, data gathering and reduction procedures for thermal and chemical property characterization for the new candidate material are discussed. Difficulties with test procedures, implementation of properties into the Charring Material Ablator (CMA) codes, and results correlation with static motor fire data are provided. After two successful five segment motor firings using the PBI/NBR insulator, performance results for the new material look good and the material should eventually be qualified for man rated use in large solid rocket motor applications.

Evaluation of training programs and entry-level qualifications for nuclear-power-plant control-room personnel based on the systems approach to training

DOE Office of Scientific and Technical Information (OSTI.GOV)

Haas, P M; Selby, D L; Hanley, M J

1983-09-01

This report summarizes results of research sponsored by the US Nuclear Regulatory Commission (NRC) Office of Nuclear Regulatory Research to initiate the use of the Systems Approach to Training in the evaluation of training programs and entry level qualifications for nuclear power plant (NPP) personnel. Variables (performance shaping factors) of potential importance to personnel selection and training are identified, and research to more rigorously define an operationally useful taxonomy of those variables is recommended. A high-level model of the Systems Approach to Training for use in the nuclear industry, which could serve as a model for NRC evaluation of industrymore » programs, is presented. The model is consistent with current publically stated NRC policy, with the approach being followed by the Institute for Nuclear Power Operations, and with current training technology. Checklists to be used by NRC evaluators to assess training programs for NPP control-room personnel are proposed which are based on this model.« less

Transparency of Vocational Qualifications: The Leonardo da Vinci Approach. CEDEFOP Panorama Series.

ERIC Educational Resources Information Center

Bjornavold, Jens; Pettersson, Sten

This report gives an overview of the situation of transparency of vocational qualifications by presenting measures introduced at the European Community level and by drawing attention to projects within the Leonardo da Vinci Program dealing with the issue. A 16-page executive summary appears first. Chapter 1 provides general background and aims.…

Relative quantitative comparisons of the extracellular protein profiles of Staphylococcus aureus UAMS-1 and its sarA, agr, and sarA agr regulatory mutants using one-dimensional polyacrylamide gel electrophoresis and nanocapillary liquid chromatography coupled with tandem mass spectrometry.

PubMed

Jones, Richard C; Deck, Joanna; Edmondson, Ricky D; Hart, Mark E

2008-08-01

One-dimensional polyacrylamide gel electrophoresis followed by nanocapillary liquid chromatography coupled with mass spectrometry was used to analyze proteins isolated from Staphylococcus aureus UAMS-1 after 3, 6, 12, and 24 h of in vitro growth. Protein abundance was determined using a quantitative value termed normalized peptide number, and overall, proteins known to be associated with the cell wall were more abundant early on in growth, while proteins known to be secreted into the surrounding milieu were more abundant late in growth. In addition, proteins from spent media and cell lysates of strain UAMS-1 and its isogenic sarA, agr, and sarA agr regulatory mutant strains during exponential growth were identified, and their relative abundances were compared. Extracellular proteins known to be regulated by the global regulators sarA and agr displayed protein levels in accordance with what is known regarding the effects of these regulators. For example, cysteine protease (SspB), endopeptidase (SspA), staphopain (ScpA), and aureolysin (Aur) were higher in abundance in the sarA and sarA agr mutants than in strain UAMS-1. The immunoglobulin G (IgG)-binding protein (Sbi), immunodominant staphylococcal antigen A (IsaA), IgG-binding protein A (Spa), and the heme-iron-binding protein (IsdA) were most abundant in the agr mutant background. Proteins whose abundance was decreased in the sarA mutant included fibrinogen-binding protein (Fib [Efb]), IsaA, lipase 1 and 2, and two proteins identified as putative leukocidin F and S subunits of the two-component leukotoxin family. Collectively, this approach identified 1,263 proteins (matches of two peptides or more) and provided a convenient and reliable way of identifying proteins and comparing their relative abundances.

Complete Genome Sequence of the MRSA Isolate HC1335 from ST239 Lineage Displaying a Truncated AgrC Histidine Kinase Receptor

PubMed Central

Botelho, Ana M. N.; Costa, Maiana O. C.; Beltrame, Cristiana O.; Ferreira, Fabienne A.; Lima, Nicholas C. B.; Costa, Bruno S. S.; de Morais, Guilherme L.; Souza, Rangel C.; Almeida, Luiz G. P.; Vasconcelos, Ana T. R.; Nicolás, Marisa F.; Figueiredo, Agnes M. S.

2016-01-01

Methicillin-resistant Staphylococcus aureus (MRSA) is still one of the most important hospital pathogen globally. The multiresistant isolates of the ST239-SCCmecIII lineage are spread over large geographic regions, colonizing and infecting hospital patients in virtually all continents. The balance between fitness (adaptability) and virulence potential is likely to represent an important issue in the clonal shift dynamics leading the success of some specific MRSA clones over another. The accessory gene regulator (agr) is the master quorum sensing system of staphylococci playing a role in the global regulation of key virulence factors. Consequently, agr inactivation in S. aureus may represent a significant mechanism of genetic variability in the adaptation of this healthcare-associated pathogen. We report here the complete genome sequence of the methicillin-resistant S. aureus, isolate HC1335, a variant of the ST239 lineage, which presents a natural insertion of an IS256 transposase element in the agrC gene encoding AgrC histidine kinase receptor. PMID:27635055

Qualification of safety-related electrical equipment in France. Methods, approach and test facilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Raimondo, E.; Capman, J.L.; Herovard, M.

1985-05-01

Requirements for qualification of electrical equipment used in French-built nuclear power plants are stated in a national code, the RCC-E, or Regles de Construction et de Conception des Materiels Electriques. Under the RCC-E, safety related equipment is assigned to one of three different categories, according to location in the plant and anticipated normal, accident and post-accident behavior. Qualification tests differ for each category and procedures range in scope from the standard seismic test to the highly stringent VISA program, which specifies a predetermined sequence of aging, radiation, seismic and simulated accident testing. A network of official French test facilities wasmore » developed specifically to meet RCC-E requirements.« less

25 CFR 170.506 - What are the minimum qualifications for certified bridge inspectors?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false What are the minimum qualifications for certified bridge inspectors? 170.506 Section 170.506 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR LAND AND WATER INDIAN RESERVATION ROADS PROGRAM Planning, Design, and Construction of Indian Reservation Roads...

The "Qualifications Business" in Higher Education

ERIC Educational Resources Information Center

Berkhout, S. J.

2006-01-01

Qualifications have become academic currency. Apart from adding to the success and fluency with which people can move or be transferred from job to job, nationally and internationally, qualifications also serve to shape individuals' perceptions of their own worth because of their impact on their holders' expectations and prospects, as well as…

Cost of Meeting House and Senate Proposed Head Start Teacher Qualification Requirements

ERIC Educational Resources Information Center

Center for Law and Social Policy, Inc. (CLASP), 2005

2005-01-01

This analysis provides a preliminary estimate of the necessary level of funding needed to raise the degree qualifications to meet the requirements in the Head Start reauthorization legislation currently proposed in the House and Senate. While each bill is designed to improve the quality of Head Start programs by requiring an increase in the number…

Inspection qualification (IQ) and operational qualification (OQ) for a vacuum freeze-dryer--Part I. General discussion.

PubMed

Jennings, T A; Scheer, A; Emodi, A; Puderbach, L; King, S; Norton, T

1996-01-01

The principle objectives of this paper are (a), to develop the rationale for conducting an inspection qualification (IQ) and operational qualification (OQ) of a vacuum freeze-dryer; (b), to identify the key elements that require verification for completion of the IQ; and (c), to establish the necessary environmental and operational parameters necessary for the OQ of the vacuum freeze-dryer.

47 CFR 54.415 - Consumer qualification for Link Up.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 3 2010-10-01 2010-10-01 false Consumer qualification for Link Up. 54.415... (CONTINUED) UNIVERSAL SERVICE Universal Service Support for Low-Income Consumers § 54.415 Consumer qualification for Link Up. (a) In a state that mandates state Lifeline support, the consumer qualification...

46 CFR 57.05-2 - Transfer of performance qualifications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Transfer of performance qualifications. 57.05-2 Section 57.05-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-2 Transfer of performance qualifications. (a) The...

46 CFR 57.05-2 - Transfer of performance qualifications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Transfer of performance qualifications. 57.05-2 Section 57.05-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-2 Transfer of performance qualifications. (a) The...

46 CFR 57.05-2 - Transfer of performance qualifications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Transfer of performance qualifications. 57.05-2 Section 57.05-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-2 Transfer of performance qualifications. (a) The...

46 CFR 57.05-2 - Transfer of performance qualifications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Transfer of performance qualifications. 57.05-2 Section 57.05-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-2 Transfer of performance qualifications. (a) The...

46 CFR 57.05-2 - Transfer of performance qualifications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Transfer of performance qualifications. 57.05-2 Section 57.05-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-2 Transfer of performance qualifications. (a) The...

47 CFR 54.415 - Consumer qualification for Link Up.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 3 2011-10-01 2011-10-01 false Consumer qualification for Link Up. 54.415... (CONTINUED) UNIVERSAL SERVICE Universal Service Support for Low-Income Consumers § 54.415 Consumer qualification for Link Up. (a) In a state that mandates state Lifeline support, the consumer qualification...

APM for a Constellation Intersatellite Link - EM Qualification and Lessons Learned

NASA Technical Reports Server (NTRS)

Hartel, Frank; Kozilek, Horst

2016-01-01

For an Intersatellite Link (ISL) of a future constellation program, a study phase was initiated by ESA to design a mechanism for Radio Frequency communication. Airbus DS Friedrichshafen (ADSF) proposed a design based on the Antenna Pointing Mechanism (APM) family with modifications that met the stated needs of the constellation. A qualification program was started beginning in September 2015 to verify the launch and thermal loads and the equipment performance (Radio Frequency, Pointing, Microvibration and Magnetic Moment). Technical challenges identified with the Engineering Model will be discussed within this paper.

Space Qualification Test of a-Silicon Solar Cell Modules

NASA Technical Reports Server (NTRS)

Kim, Q.; Lawton, R. A.; Manion, S. J.; Okuno, J. O.; Ruiz, R. P.; Vu, D. T.; Vu, D. T.; Kayali, S. A.; Jeffrey, F. R.

2004-01-01

The basic requirements of solar cell modules for space applications are generally described in MIL-S-83576 for the specific needs of the USAF. However, the specifications of solar cells intended for use on space terrestrial applications are not well defined. Therefore, this qualifications test effort was concentrated on critical areas specific to the microseismometer probe which is intended to be included in the Mars microprobe programs. Parameters that were evaluated included performance dependence on: illuminating angles, terrestrial temperatures, lifetime, as well as impact landing conditions. Our qualification efforts were limited to these most critical areas of concern. Most of the tested solar cell modules have met the requirements of the program except the impact tests. Surprisingly, one of the two single PIN 2 x 1 amorphous solar cell modules continued to function even after the 80000G impact tests. The output power parameters, Pout, FF, Isc and Voc, of the single PIN amorphous solar cell module were found to be 3.14 mW, 0.40, 9.98 mA and 0.78 V, respectively. These parameters are good enough to consider the solar module as a possible power source for the microprobe seismometer. Some recommendations were made to improve the usefulness of the amorphous silicon solar cell modules in space terrestrial applications, based on the results obtained from the intensive short term lab test effort.

On the Functions of a System of Vocational Qualifications

ERIC Educational Resources Information Center

Lum, Gerard

2015-01-01

While acknowledging that a system of vocational qualifications might be perceived as having any number of possible purposes, this paper identifies three primary functions that any vocational qualification must fulfil by dint of being a vocational qualification. It is argued that current arrangements are unable to fulfil these essential functions…

Vancomycin modifies the expression of the agr system in multidrug-resistant Staphylococcus aureus clinical isolates

PubMed Central

Cázares-Domínguez, Vicenta; Ochoa, Sara A.; Cruz-Córdova, Ariadnna; Rodea, Gerardo E.; Escalona, Gerardo; Olivares, Alma L.; Olivares-Trejo, José de Jesús; Velázquez-Guadarrama, Norma; Xicohtencatl-Cortes, Juan

2015-01-01

Staphylococcus aureus is an opportunistic pathogen that colonizes human hosts and causes a wide variety of diseases. Two interacting regulatory systems called agr (accessory gene regulator) and sar (staphylococcal accessory regulator) are involved in the regulation of virulence factors. The aim of this study was to evaluate the effect of vancomycin on hld and spa gene expression during the exponential and post-exponential growth phases in multidrug-resistant (MDR) S. aureus. Methods: Antibiotic susceptibility was evaluated by the standard microdilution method. The phylogenetic profile was obtained by pulsed-field gel electrophoresis (PFGE). Polymorphisms of agr and SCCmec (staphylococcal cassette chromosome mec) were analyzed by multiplex polymerase chain reaction (PCR). The expression levels of hld and spa were analyzed by reverse transcription-PCR. An enzyme-linked immunosorbent assay (ELISA) was performed to detect protein A, and biofilm formation was analyzed via crystal violet staining. Results: In total, 60.60% (20/33) of S. aureus clinical isolates were MDR. Half (10/20) of the MDR S. aureus isolates were distributed in subcluster 10, with >90% similarity among them. In the isolates of this subcluster, a high prevalence (100%) for the agrII and the cassette SCCmec II polymorphisms was found. Our data showed significant increases in hld expression during the post-exponential phase in the presence and absence of vancomycin. Significant increases in spa expression, protein A production and biofilm formation were observed during the post-exponential phase when the MDR S. aureus isolates were challenged with vancomycin. Conclusion: The polymorphism agrII, which is associated with nosocomial isolates, was the most prevalent polymorphism in MDR S. aureus. Additionally, under our study conditions, vancomycin modified hld and spa expression in these clinical isolates. Therefore, vancomycin may regulate alternative systems that jointly participate in the regulation of

Meeting the Challenges of Literacy Difficulties: Developing a Qualification for Specialist Literacy Teachers.

ERIC Educational Resources Information Center

Limbrick, Libby; Parkhill, Faye; Smith, John

In 2001, the New Zealand Ministry of Education awarded a contract to a consortium of three colleges of education to provide a training program that would lead to a new national literacy qualification for a newly developed specialist literacy teaching position--Resource Teacher: Literacy. Resource Teachers of Literacy are skilled and experienced…

Building the Qualification File of EGNOS with DOORS

NASA Astrophysics Data System (ADS)

Fabre, J.

2008-08-01

EGNOS, the European Satellite-Based Augmentation System (SBAS) to GPS, is getting to its final deployment and being initially operated towards qualification and certification to reach operational capability by 2008/2009. A very important milestone in the development process is the System Qualification Review (QR). As the verification phase aims at demonstrating that the EGNOS System design meets the applicable requirements, the QR declares the completion of verification activities. The main document to present at QR is a consolidated, consistent and complete Qualification file. The information included shall give confidence to the QR reviewers that the performed qualification activities are completed. Therefore, an important issue for the project team is to focus on synthetic and consistent information, and to make the presentation as clear as possible. Traceability to applicable requirements shall be systematically presented. Moreover, in order to support verification justification, reference to details shall be available, and the reviewer shall have the possibility to link automatically to the documents including this detailed information. In that frame, Thales Alenia Space has implemented a strong support in terms of methodology and tool, to provide to System Engineering and Verification teams a single reference technical database, in which all team members consult the applicable requirements, compliance, justification, design data and record the information necessary to build the final Qualification file. This paper presents the EGNOS context, the Qualification file contents, and the methodology implemented, based on Thales Alenia Space practices and in line with ECSS. Finally, it shows how the Qualification file is built in a DOORS environment.

14 CFR 60.15 - Initial qualification requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Initial qualification requirements. 60.15 Section 60.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.15...

14 CFR 60.15 - Initial qualification requirements.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Initial qualification requirements. 60.15 Section 60.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.15...

14 CFR 60.7 - Sponsor qualification requirements.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Sponsor qualification requirements. 60.7 Section 60.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.7...

14 CFR 60.7 - Sponsor qualification requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Sponsor qualification requirements. 60.7 Section 60.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.7...

14 CFR 60.7 - Sponsor qualification requirements.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Sponsor qualification requirements. 60.7 Section 60.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.7...

14 CFR 60.15 - Initial qualification requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Initial qualification requirements. 60.15 Section 60.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.15...

14 CFR 60.7 - Sponsor qualification requirements.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Sponsor qualification requirements. 60.7 Section 60.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.7...

14 CFR 60.15 - Initial qualification requirements.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Initial qualification requirements. 60.15 Section 60.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.15...

14 CFR 60.4 - Qualification Performance Standards.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Qualification Performance Standards. 60.4 Section 60.4 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.4...

14 CFR 60.4 - Qualification Performance Standards.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Qualification Performance Standards. 60.4 Section 60.4 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.4...

14 CFR 60.15 - Initial qualification requirements.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Initial qualification requirements. 60.15 Section 60.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.15...

14 CFR 60.4 - Qualification Performance Standards.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Qualification Performance Standards. 60.4 Section 60.4 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.4...

14 CFR 60.4 - Qualification Performance Standards.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Qualification Performance Standards. 60.4 Section 60.4 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.4...

14 CFR 60.4 - Qualification Performance Standards.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Qualification Performance Standards. 60.4 Section 60.4 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.4...

48 CFR 1437.7001 - Contractor qualification requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contractor qualification requirements. 1437.7001 Section 1437.7001 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR... Contractor qualification requirements. (a) Prior to award of a contract for real property appraisal services...

Large Bore Powder Gun Qualification (U)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rabern, Donald A.; Valdiviez, Robert

A Large Bore Powder Gun (LBPG) is being designed to enable experimentalists to characterize material behavior outside the capabilities of the NNSS JASPER and LANL TA-55 PF-4 guns. The combination of these three guns will create a capability to conduct impact experiments over a wide range of pressures and shock profiles. The Large Bore Powder Gun will be fielded at the Nevada National Security Site (NNSS) U1a Complex. The Complex is nearly 1000 ft below ground with dedicated drifts for testing, instrumentation, and post-shot entombment. To ensure the reliability, safety, and performance of the LBPG, a qualification plan has beenmore » established and documented here. Requirements for the LBPG have been established and documented in WE-14-TR-0065 U A, Large Bore Powder Gun Customer Requirements. The document includes the requirements for the physics experiments, the gun and confinement systems, and operations at NNSS. A detailed description of the requirements is established in that document and is referred to and quoted throughout this document. Two Gun and Confinement Systems will be fielded. The Prototype Gun will be used primarily to characterize the gun and confinement performance and be the primary platform for qualification actions. This gun will also be used to investigate and qualify target and diagnostic modifications through the life of the program (U1a.104 Drift). An identical gun, the Physics Gun, will be fielded for confirmatory and Pu experiments (U1a.102D Drift). Both guns will be qualified for operation. The Gun and Confinement System design will be qualified through analysis, inspection, and testing using the Prototype Gun for the majority of process. The Physics Gun will be qualified through inspection and a limited number of qualification tests to ensure performance and behavior equivalent to the Prototype gun. Figure 1.1 shows the partial configuration of U1a and the locations of the Prototype and Physics Gun/Confinement Systems.« less

NARC Rayon Replacement Program for the RSRM Nozzle, Phase IV Qualification and Implementation Status

NASA Technical Reports Server (NTRS)

Haddock, M. Reed; Wendel, Gary M.; Cook, Roger V.

2005-01-01

The Space Shuttle NARC Rayon Replacement Program has down-selected Enka rayon as a replacement for the obsolete NARC rayon in the nozzle carbon cloth phenolic (CCP) ablative insulators. Full qualification testing of the Enka rayon-based carbon cloth phenolic is underway, including processing, thmal/structural properties, and hot-fire subscale tests. Required thermal-structural capabilities, together with confidence in erosio/char performance in simulated and subscale hot fire tests such as Wright-Patterson Air Force Base Laser Hardened Materials Evaluation Laboratory testing, NASA-MSFC 24-inch motor tests, NASA-MSFC Solid Fuel Torch - Super Sonic Blast Tube, NASA-MSFC Plasma Torch Test Bed, ATK Thiokol Forty Pound Charge and NASA-MSFC MNASA justified the testing of the new Enka-rayon candidate on full-scale static test motors. The first RSRM full-scale static test motor nozzle, fabricated using the new Enka rayon-based CCP, was successfully demonstrated in June 2004. Two additional static test motors are planned with the new Enka rayon in the next two years along with additional A-basis property characterization. Process variation or "corner-of-the-box" testing together with cured and uncured aging studies are also planned as some of the pre-flight implementation activities with 5-year cured aging studies over-lapping flight hardware fabrication.

16 CFR § 1210.14 - Qualification testing.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Qualification testing. § 1210.14 Section § 1210.14 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS SAFETY STANDARD FOR CIGARETTE LIGHTERS Certification Requirements § 1210.14 Qualification testing. (a...

48 CFR 34.103 - Testing and qualification.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Developed Under Title III, Defense Production Act 34.103 Testing and qualification. (a) Contractors... or item of supply; and (2) for major systems in production, remaining quantities to be acquired are sufficient to justify incurring the cost of testing and qualification. In evaluating this request, the...

Impact of the Europeanisation of Education: Qualifications Frameworks in Europe

ERIC Educational Resources Information Center

Mikulec, Borut

2017-01-01

This article examines the influence of the European qualifications framework--a key European lifelong learning policy instrument for improving employability, comparability and mobility in the European educational space--on the establishment of national qualifications frameworks in Europe. The European qualifications framework and national…

49 CFR 213.345 - Vehicle qualification testing.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Vehicle qualification testing. 213.345 Section 213... Higher § 213.345 Vehicle qualification testing. (a) All rolling stock types which operate at Class 6... demonstrate that the vehicle dynamic response to track alinement and geometry variations are within acceptable...

30 CFR 281.4 - Qualifications of lessees.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Qualifications of lessees. 281.4 Section 281.4 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR OFFSHORE LEASING OF MINERALS OTHER THAN OIL, GAS, AND SULPHUR IN THE OUTER CONTINENTAL SHELF General § 281.4 Qualifications of...

Anechoic chamber qualification at ultrasonic frequencies

NASA Astrophysics Data System (ADS)

Jenny, Trevor; Anderson, Brian

2010-10-01

Qualifying an anechoic chamber for frequencies that extend into the ultrasonic range is necessary for research work involving airborne ultrasonic sound. For example, an anechoic chamber allows for measurements of the direct sound radiated by an object without reflections from walls. The ANSI S12.55/ISO 3745 standard which covers anechoic chamber qualification does not extend into the ultrasonic frequency range, nor have others discussed this frequency range in the literature. An increasing number of technologies are employing ultrasound; hence the need to develop facilities to conduct basic research studies on airborne ultrasound. This presentation will discuss the challenges associated with chamber qualification and present the results for qualification of a chamber at Brigham Young University. [This work has been funded by the Los Alamos National Laboratory

Year One Summary of X-energy Pebble Fuel Development at ORNL

DOE Office of Scientific and Technical Information (OSTI.GOV)

Helmreich, Grant W.; Hunn, John D.; McMurray, Jake W.

2017-06-01

The Advanced Reactor Concepts X-energy (ARC-Xe) Pebble Fuel Development project at Oak Ridge National Laboratory (ORNL) has successfully completed its first year, having made excellent progress in accomplishing programmatic objectives. The primary focus of research at ORNL in support of X-energy has been the training of X-energy fuel fabrication engineers and the establishment of US pebble fuel production capabilities able to supply the Xe-100 pebble-bed reactor. These efforts have been strongly supported by particle fuel fabrication and characterization expertise present at ORNL from the Advanced Gas Reactor (AGR) Fuel Development and Qualification Program.

10 CFR 1046.11 - Medical and physical fitness qualification standards.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Medical and physical fitness qualification standards. 1046... INTERESTS Protective Force Personnel § 1046.11 Medical and physical fitness qualification standards. (a... personnel any individual who fails to meet the applicable medical and physical fitness qualification...

10 CFR 1046.11 - Medical and physical fitness qualification standards.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Medical and physical fitness qualification standards. 1046... INTERESTS Protective Force Personnel § 1046.11 Medical and physical fitness qualification standards. (a... personnel any individual who fails to meet the applicable medical and physical fitness qualification...

10 CFR 1046.11 - Medical and physical fitness qualification standards.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Medical and physical fitness qualification standards. 1046... INTERESTS Protective Force Personnel § 1046.11 Medical and physical fitness qualification standards. (a... personnel any individual who fails to meet the applicable medical and physical fitness qualification...

The European Qualifications Framework: A Technical Critique

ERIC Educational Resources Information Center

Lester, Stan

2015-01-01

The European Qualifications Framework (EQF) was introduced in 2008 as a "meta-framework" or common reference point for national qualifications frameworks in Europe, a function for which, with some caveats, it has been pragmatically successful. It has also been used with variable success to support the development or referencing of…

30 CFR 880.11 - Qualifications of projects.

Code of Federal Regulations, 2010 CFR

2010-07-01

... ABANDONED MINE LAND RECLAMATION MINE FIRE CONTROL § 880.11 Qualifications of projects. The purpose of all projects is to prevent injury and loss of life, protect public health, conserve natural resources, or... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Qualifications of projects. 880.11 Section 880...

46 CFR 68.37 - Cessation of qualifications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... DOCUMENTATION OF VESSELS: EXCEPTIONS TO COASTWISE QUALIFICATION Documentation of Certain Vessels for Oil Spill Cleanup § 68.37 Cessation of qualifications. (a) If the vessel is owned by a not-for-profit oil spill... change. The not-for-profit oil spill response cooperative shall report the change in writing to the...

46 CFR 68.37 - Cessation of qualifications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... DOCUMENTATION OF VESSELS: EXCEPTIONS TO COASTWISE QUALIFICATION Documentation of Certain Vessels for Oil Spill Cleanup § 68.37 Cessation of qualifications. (a) If the vessel is owned by a not-for-profit oil spill... change. The not-for-profit oil spill response cooperative shall report the change in writing to the...

46 CFR 68.37 - Cessation of qualifications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... DOCUMENTATION OF VESSELS: EXCEPTIONS TO COASTWISE QUALIFICATION Documentation of Certain Vessels for Oil Spill Cleanup § 68.37 Cessation of qualifications. (a) If the vessel is owned by a not-for-profit oil spill... change. The not-for-profit oil spill response cooperative shall report the change in writing to the...

46 CFR 57.05-4 - Welder qualification by procedure tests.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Welder qualification by procedure tests. 57.05-4 Section 57.05-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-4 Welder qualification by procedure tests...

46 CFR 57.05-4 - Welder qualification by procedure tests.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Welder qualification by procedure tests. 57.05-4 Section 57.05-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-4 Welder qualification by procedure tests...

46 CFR 57.05-4 - Welder qualification by procedure tests.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Welder qualification by procedure tests. 57.05-4 Section 57.05-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-4 Welder qualification by procedure tests...

46 CFR 57.05-4 - Welder qualification by procedure tests.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Welder qualification by procedure tests. 57.05-4 Section 57.05-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-4 Welder qualification by procedure tests...

46 CFR 57.05-4 - Welder qualification by procedure tests.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Welder qualification by procedure tests. 57.05-4 Section 57.05-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-4 Welder qualification by procedure tests...

SRS H1616 hydride transport vessel qualification report

DOT National Transportation Integrated Search

1998-12-01

This report serves as the design qualification basis for both transport and facility use. headings identify report sections as containing qualification information for transport use, facility use, or both transport and facility use. This report demon...

Test report: Electron-proton spectrometer qualification test unit, qualification test

NASA Technical Reports Server (NTRS)

Vincent, D. L.

1972-01-01

Qualification tests of the electron-proton spectrometer test unit are presented. The tests conducted were: (1) functional, (2) thermal/vacuum, (3) electromagnetic interference, (4) acoustic, (5) shock, (6) vibration, and (7) humidity. Results of each type of test are presented in the form of data sheets.

Vocations: The Link between Post-Compulsory Education and the Labour Market. What the Research Says For... Qualification & Approval Bodies

ERIC Educational Resources Information Center

Wheelahan, Leesa; Buchanan, John; Yu, Serena

2015-01-01

This summary pulls together the relevant key findings for qualification and approval bodies from the research program "Vocations: The Link between Post-Compulsory Education and the Labour Market." The program was comprised of three different strands: (1) pathways from VET in Schools, (2) pathways within and between vocational education…

Qualifications and Competencies for Population Health Management Positions: A Content Analysis of Job Postings.

PubMed

Meyer, Melanie

2017-12-01

The need for population health management expertise has increased as the health care industry shifts toward value-based care. However, many organizations report hiring gaps as they seek to fill positions. The purpose of this study was to analyze the types of population health management positions for which health care organizations are hiring, including qualifications and competencies required for these positions. A content analysis was conducted on 271 job postings collected during a 2-month period. A typology of qualifications and competencies was developed based on the content analysis. Profiles were generated for the top 5 job title classifications: directors, coordinators, care managers, analysts, and specialists. This study highlights the investment health care organizations are making in population health management and the prominent role these positions are playing in the health care environment today. Many organizations are building out population health management teams resulting in multiple positions at different levels being added. As the market demands competent candidates who are equipped with specialized population health expertise as well as practical experience in program development, technology applications, care management, and analytics, professional education programs will need to adapt curricula to address the required areas. Competencies for specific job title classifications may need further evaluation and refinement over time. Study results can be used by organizations for strategic planning, by educators to target needed qualifications and competencies, and by researchers and policy advisors to assess progress toward value-based care.

Qualifications Frameworks in Africa: A Critical Reflection

ERIC Educational Resources Information Center

Higgs, P.; Keevy, J.

2009-01-01

Today there is an accelerating trend towards qualifications frameworks as an instrument to develop, classify and recognise formal learning across the African continent, as is also the case across most of Europe, Australasia and the Asia-Pacific region. As more and more countries and regions across the world develop qualifications frameworks to…

49 CFR 214.347 - Training and qualification for lone workers.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Training and qualification for lone workers. 214... § 214.347 Training and qualification for lone workers. Each lone worker shall be trained and qualified... training and qualification for lone workers shall include, as a minimum, consideration of the following...

49 CFR 214.347 - Training and qualification for lone workers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Training and qualification for lone workers. 214... § 214.347 Training and qualification for lone workers. Each lone worker shall be trained and qualified... training and qualification for lone workers shall include, as a minimum, consideration of the following...

The Agr quorum-sensing system regulates fibronectin binding but not hemolysis in the absence of a functional electron transport chain.

PubMed

Pader, Vera; James, Ellen H; Painter, Kimberley L; Wigneshweraraj, Sivaramesh; Edwards, Andrew M

2014-10-01

Staphylococcus aureus is responsible for numerous chronic and recurrent infections, which are frequently associated with the emergence of small-colony variants (SCVs) that lack a functional electron transport chain. SCVs exhibit enhanced expression of fibronectin-binding protein (FnBP) and greatly reduced hemolysin production, although the basis for this is unclear. One hypothesis is that these phenotypes are a consequence of the reduced Agr activity of SCVs, while an alternative is that the lack of a functional electron transport chain and the resulting reduction in ATP production are responsible. Disruption of the electron transport chain of S. aureus genetically (hemB and menD) or chemically, using 2-n-heptyl-4-hydroxyquinoline N-oxide (HQNO), inhibited both growth and Agr activity and conferred an SCV phenotype. Supplementation of the culture medium with synthetic autoinducing peptide (sAIP) significantly increased Agr expression in both hemB mutant strains and S. aureus grown with HQNO and significantly reduced staphylococcal adhesion to fibronectin. However, sAIP did not promote hemolysin expression in hemB mutant strains or S. aureus grown with HQNO. Therefore, while Agr regulates fibronectin binding in SCVs, it cannot promote hemolysin production in the absence of a functional electron transport chain. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

The Agr Quorum-Sensing System Regulates Fibronectin Binding but Not Hemolysis in the Absence of a Functional Electron Transport Chain

PubMed Central

Pader, Vera; James, Ellen H.; Painter, Kimberley L.; Wigneshweraraj, Sivaramesh

2014-01-01

Staphylococcus aureus is responsible for numerous chronic and recurrent infections, which are frequently associated with the emergence of small-colony variants (SCVs) that lack a functional electron transport chain. SCVs exhibit enhanced expression of fibronectin-binding protein (FnBP) and greatly reduced hemolysin production, although the basis for this is unclear. One hypothesis is that these phenotypes are a consequence of the reduced Agr activity of SCVs, while an alternative is that the lack of a functional electron transport chain and the resulting reduction in ATP production are responsible. Disruption of the electron transport chain of S. aureus genetically (hemB and menD) or chemically, using 2-n-heptyl-4-hydroxyquinoline N-oxide (HQNO), inhibited both growth and Agr activity and conferred an SCV phenotype. Supplementation of the culture medium with synthetic autoinducing peptide (sAIP) significantly increased Agr expression in both hemB mutant strains and S. aureus grown with HQNO and significantly reduced staphylococcal adhesion to fibronectin. However, sAIP did not promote hemolysin expression in hemB mutant strains or S. aureus grown with HQNO. Therefore, while Agr regulates fibronectin binding in SCVs, it cannot promote hemolysin production in the absence of a functional electron transport chain. PMID:25092909

Implementing a National Qualifications Framework in Lithuania

ERIC Educational Resources Information Center

Tutlys, Vidmantas; Spudyte, Irma

2011-01-01

The design of the national qualifications framework (NQF) in Lithuania started in 2006. The NQF was officially approved by the government decree in May 2010. This article explores the influence of the processes of institutional change on the reform of the national system of qualifications in Lithuania through the implementation of the NQF, looking…

BSM Delta qualification 2, volume 1

NASA Technical Reports Server (NTRS)

1994-01-01

This report, presented in three volumes, provides the results of a two-motor Delta Qualification 2 program conducted in 1993 to certify the following enhancements for incorporation into Booster Separation Motor (BSM) flight hardware: (1) vulcanized-in-place nozzle aft closure insulation; (2) new isostatic ATJ bulk graphite throat insert material; (3) adhesive EA 9394 for bonding the nozzle throat, igniter grain rod/centering insert/igniter case; (4) deletion of the igniter adapter insulator ring; (5) deletion of igniter adapter/igniter case interface RTV; and (6) deletion of Loctite from igniter retainer plate threads. The enhancements above directly resulted from (1) the BSM Total Quality Management (TQM) Team initiatives to enhance the BSM producibility, and (2) the necessity to qualify new throat insert and adhesive systems to replace existing materials that will not be available. Testing was completed at both the component and motor levels. Component testing was accomplished to screen candidate materials (e.g., throat materials, adhesive systems) and to optimize processes (e.g., aft closure insulator vulcanization approach) prior to their incorporation into the test motors. Motor testing - consisting of two motors, randomly selected by USBI's onsite quality personnel from production lot AAY, which were modified to accept the enhancements - were completed to provide the final qualification of the enhancements for incorporation into flight hardware. It is concluded that all of the enhancements herein tested are qualified to be incorporated into flight hardware for the BSM.

BSM Delta Qualification 2, volume 2

NASA Technical Reports Server (NTRS)

1994-01-01

This report, presented in three volumes, provides the results of a two-motor Delta Qualification 2 program conducted in 1993 to certify the following enhancements for incorporation into booster separation motor (BSM) flight hardware: vulcanized-in-place nozzle aft closure insulation; new iso-static ATJ bulk graphite throat insert material; adhesive EA 9394 for bonding the nozzle throat, igniter grain rod/centering insert/igniter case; deletion of the igniter adapter insulator ring; deletion of the igniter adapter/igniter case interface RTV; and deletion of loctite from igniter retainer plate threads. The enhancements above directly resulted from (1) the BSM total quality management (TQM) team initiatives to enhance the BSM producibility, and (2) the necessity to qualify new throat insert and adhesive systems to replace existing materials that will not be available. Testing was completed at both the component and motor levels. Component testing was accomplished to screen candidate materials (e.g., throat materials, adhesive systems) and to optimize processes (e.g., aft closure insulator vulcanization approach) prior to their incorporation into the test motors. Motor tests -- consisting of two motors, randomly selected by USBI's on-site quality personnel from production lot AAY, which were modified to accept the enhancements -- were completed to provide the final qualification of the enhancements for incorporation into flight hardware. Volume 2 details the environmental testing (vibration and shock) conducted at Marshall Space Flight Center (MSFC) to which the motors were subjected prior to static tests.

Governance: current trends in board education, competencies, and qualifications.

PubMed

Nash, David B; Murphy, Sean Patrick; Mullaney, Anne D

2011-01-01

Hospitals and health system boards have been under enormous pressure to be more accountable. However, there is now an emerging trend of voluntary, mandatory, and payer-driven board education programs. Currently, 13 states have some type of formal, organized board education and development initiative, 10 of which are classified as "director and/ or board certificate" or "certification" programs; the American Hospital Association (through and with its affiliated Center for Healthcare Governance) has issued a Blue Ribbon Panel Report on core competencies. Finally, both Congress and the Securities and Exchange Commission require publicly traded companies to disclose board and director qualifications. This article reviews these emerging trends and posits that these developments are a necessary prerequisite not only for accountability and better governance but also for quality care.

Job Outcomes among Korean Graduates with Vocational Qualifications

ERIC Educational Resources Information Center

Lee, Young-Min; Cho, Sung-Eun

2017-01-01

Purpose: The purpose of this paper is to ascertain the status of recent Korean four-year college students' acquisition of vocational qualification certificates (hereinafter "certificates" or "qualifications"), the factors that influence certificate acquisition, and the resulting employment and wage effects.…

Extraction-Scrub-Strip test results from the interim Salt Disposition Program Macrobatch 9 Tank 21H qualification samples

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peters, T.

2016-02-23

Savannah River National Laboratory (SRNL) analyzed samples from Tank 21H in support of qualification of Macrobatch (Salt Batch) 9 for the Interim Salt Disposition Program (ISDP). The Salt Batch 9 characterization results were previously reported. An Extraction-Scrub-Strip (ESS) test was performed to determine cesium distribution ratios (D (Cs)) and cesium concentration in the strip effluent and decontaminated salt solution (DSS) streams; this data will be used by Tank Farm Engineering to project a cesium decontamination factor (DF). This test used actual Tank 21H material, and a blend solvent prepared by SRNL that mimics the solvent composition currently being used atmore » the Modular Caustic-Side Solvent Extraction Unit (MCU). The ESS test showed acceptable performance with an extraction D (Cs) value of 52.4. This value is consistent with results from previous salt batch ESS tests using similar solvent formulations. This compares well against the predicted value of 56.5 from a recently created D (Cs) model« less

Extraction, -scrub, -strip test results from the interim salt disposition program macrobatch 10 tank 21H qualification samples

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peters, T. B.

Savannah River National Laboratory (SRNL) analyzed samples from Tank 21H in support of qualification of Macrobatch (Salt Batch) 10 for the Interim Salt Disposition Program (ISDP). The Salt Batch 10 characterization results were previously reported.ii,iii An Extraction, -Scrub, -Strip (ESS) test was performed to determine cesium distribution ratios (D(Cs)) and cesium concentration in the strip effluent (SE) and decontaminated salt solution (DSS) streams; this data will be used by Tank Farm Engineering to project a cesium decontamination factor (DF). This test used actual Tank 21H material, and a sample of the NGS Blend solvent currently being used at the Modularmore » Caustic-Side Solvent Extraction Unit (MCU). The ESS test showed acceptable performance with an extraction D(Cs) value of 110. This value is consistent with results from previous salt batch ESS tests using similar solvent formulations. This is better than the predicted value of 39.8 from a recently created D(Cs) model.« less

42 CFR 493.1423 - Standard; Testing personnel qualifications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 5 2013-10-01 2013-10-01 false Standard; Testing personnel qualifications. 493... Testing Laboratories Performing Moderate Complexity Testing § 493.1423 Standard; Testing personnel qualifications. Each individual performing moderate complexity testing must— (a) Possess a current license issued...

42 CFR 493.1423 - Standard; Testing personnel qualifications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 5 2011-10-01 2011-10-01 false Standard; Testing personnel qualifications. 493... Testing Laboratories Performing Moderate Complexity Testing § 493.1423 Standard; Testing personnel qualifications. Each individual performing moderate complexity testing must— (a) Possess a current license issued...

42 CFR 493.1423 - Standard; Testing personnel qualifications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 5 2014-10-01 2014-10-01 false Standard; Testing personnel qualifications. 493... Testing Laboratories Performing Moderate Complexity Testing § 493.1423 Standard; Testing personnel qualifications. Each individual performing moderate complexity testing must— (a) Possess a current license issued...

42 CFR 493.1423 - Standard; Testing personnel qualifications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 5 2012-10-01 2012-10-01 false Standard; Testing personnel qualifications. 493... Testing Laboratories Performing Moderate Complexity Testing § 493.1423 Standard; Testing personnel qualifications. Each individual performing moderate complexity testing must— (a) Possess a current license issued...

Space flight requirements for fiber optic components: qualification testing and lessons learned

NASA Astrophysics Data System (ADS)

Ott, Melanie N.; Jin, Xiaodan Linda; Chuska, Richard; Friedberg, Patricia; Malenab, Mary; Matuszeski, Adam

2006-04-01

"Qualification" of fiber optic components holds a very different meaning than it did ten years ago. In the past, qualification meant extensive prolonged testing and screening that led to a programmatic method of reliability assurance. For space flight programs today, the combination of using higher performance commercial technology, with shorter development schedules and tighter mission budgets makes long term testing and reliability characterization unfeasible. In many cases space flight missions will be using technology within years of its development and an example of this is fiber laser technology. Although the technology itself is not a new product the components that comprise a fiber laser system change frequently as processes and packaging changes occur. Once a process or the materials for manufacturing a component change, even the data that existed on its predecessor can no longer provide assurance on the newer version. In order to assure reliability during a space flight mission, the component engineer must understand the requirements of the space flight environment as well as the physics of failure of the components themselves. This can be incorporated into an efficient and effective testing plan that "qualifies" a component to specific criteria defined by the program given the mission requirements and the component limitations. This requires interaction at the very initial stages of design between the system design engineer, mechanical engineer, subsystem engineer and the component hardware engineer. Although this is the desired interaction what typically occurs is that the subsystem engineer asks the components or development engineers to meet difficult requirements without knowledge of the current industry situation or the lack of qualification data. This is then passed on to the vendor who can provide little help with such a harsh set of requirements due to high cost of testing for space flight environments. This presentation is designed to guide the

Space Flight Requirements for Fiber Optic Components; Qualification Testing and Lessons Learned

NASA Technical Reports Server (NTRS)

Ott, Melanie N.; Jin, Xiaodan Linda; Chuska, Richard; Friedberg, Patricia; Malenab, Mary; Matuszeski, Adam

2007-01-01

"Qualification" of fiber optic components holds a very different meaning than it did ten years ago. In the past, qualification meant extensive prolonged testing and screening that led to a programmatic method of reliability assurance. For space flight programs today, the combination of using higher performance commercial technology, with shorter development schedules and tighter mission budgets makes long term testing and reliability characterization unfeasible. In many cases space flight missions will be using technology within years of its development and an example of this is fiber laser technology. Although the technology itself is not a new product the components that comprise a fiber laser system change frequently as processes and packaging changes occur. Once a process or the materials for manufacturing a component change, even the data that existed on its predecessor can no longer provide assurance on the newer version. In order to assure reliability during a space flight mission, the component engineer must understand the requirements of the space flight environment as well as the physics of failure of the components themselves. This can be incorporated into an efficient and effective testing plan that "qualifies" a component to specific criteria defined by the program given the mission requirements and the component limitations. This requires interaction at the very initial stages of design between the system design engineer, mechanical engineer, subsystem engineer and the component hardware engineer. Although this is the desired interaction what typically occurs is that the subsystem engineer asks the components or development engineers to meet difficult requirements without knowledge of the current industry situation or the lack of qualification data. This is then passed on to the vendor who can provide little help with such a harsh set of requirements due to high cost of testing for space flight environments. This presentation is designed to guide the

10 CFR 603.515 - Qualification of a consortium.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS TECHNOLOGY INVESTMENT AGREEMENTS Pre-Award Business Evaluation Recipient Qualification § 603.515 Qualification of a consortium. (a) A consortium that... under the agreement. (b) If the prospective recipient of a TIA is a consortium that is not formally...

10 CFR 603.515 - Qualification of a consortium.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS TECHNOLOGY INVESTMENT AGREEMENTS Pre-Award Business Evaluation Recipient Qualification § 603.515 Qualification of a consortium. (a) A consortium that... under the agreement. (b) If the prospective recipient of a TIA is a consortium that is not formally...

10 CFR 603.515 - Qualification of a consortium.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS TECHNOLOGY INVESTMENT AGREEMENTS Pre-Award Business Evaluation Recipient Qualification § 603.515 Qualification of a consortium. (a) A consortium that... under the agreement. (b) If the prospective recipient of a TIA is a consortium that is not formally...