Sample records for qualification sample prepared

  1. Sludge batch 9 (SB9) acceptance evaluation. Radionuclide concentrations in tank 51 SB9 qualification sample prepared at SRNL

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bannochie, C. J.; Diprete, D. P.; Pareizs, J. M.

    Presented in this report are radionuclide concentrations required as part of the program of qualifying Sludge Batch 9 (SB9) for processing in the Defense Waste Processing Facility (DWPF). The SB9 material is currently in Tank 51 and has been washed and prepared for transfer to Tank 40. The acceptance evaluation needs to be completed prior to the transfer of the material in Tank 51 to Tank 40. The sludge slurry in Tank 40 has already been qualified for DWPF processing and is currently being processed as Sludge Batch 8 (SB8). The radionuclide concentrations were measured or estimated in the Tankmore » 51 SB9 Washed Qualification Sample prepared at Savannah River National Laboratory (SRNL). This sample was prepared from a three liter sample of Tank 51 sludge slurry (HTF-51-15-81) taken on July 23, 2015. The sample was delivered to SRNL where it was initially characterized in the Shielded Cells. Under the direction of Savannah River Remediation (SRR) it was then adjusted per the Tank Farm washing strategy as of October 20, 2015. This final slurry now has a composition expected to be similar to that of the slurry in Tank 51 after final preparations have been made for transfer of that slurry to Tank 40.« less

  2. Sludge batch 9 (SB9) accepance evaluation: Radionuclide concentrations in tank 51 SB9 qualification sample prepared at SRNL

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bannochie, C.; Diprete, D.; Pareizs, J.

    Presented in this report are radionuclide concentrations required as part of the program of qualifying Sludge Batch 9 (SB9) for processing in the Defense Waste Processing Facility (DWPF). The SB9 material is currently in Tank 51 and has been washed and prepared for transfer to Tank 40. The acceptance evaluation needs to be completed prior to the transfer of the material in Tank 51 to Tank 40. The sludge slurry in Tank 40 has already been qualified for DWPF processing and is currently being processed as Sludge Batch 8 (SB8). The radionuclide concentrations were measured or estimated in the Tankmore » 51 SB9 Washed Qualification Sample prepared at Savannah River National Laboratory (SRNL). This sample was prepared from a three liter sample of Tank 51 sludge slurry (HTF-51-15-81) taken on July 23, 2015. The sample was delivered to SRNL where it was initially characterized in the Shielded Cells. Under the direction of Savannah River Remediation (SRR) it was then adjusted per the Tank Farm washing strategy as of October 20, 2015. This final slurry now has a compositioniv expected to be similar to that of the slurry in Tank 51 after final preparations have been made for transfer of that slurry to Tank 40.« less

  3. Qualifications and the Professional Preparation and Development of Art Museum Educators

    ERIC Educational Resources Information Center

    Ebitz, David

    2005-01-01

    Art museums in the United States have undergone profound changes since the 1980s. Have the qualifications and professional preparation of art museum educators changed as well? This article focuses on these qualifications from five points of view: a) the changing context of art museums in transition from object-centered to visitor-centered…

  4. Preceptors' Perceptions of the Preparation and Qualifications for the Preceptor Role

    ERIC Educational Resources Information Center

    Nottingham, Sara

    2015-01-01

    Context: The 2012 Commission on Accreditation of Athletic Training Education (CAATE) standards include several changes that allow more institutional autonomy when determining the qualifications and preparation of preceptors. Clinical education coordinators (CECs) must make educated decisions in this area, yet minimal research exists to guide their…

  5. ELIMINATION OF THE CHARACTERIZATION OF DWPF POUR STREAM SAMPLE AND THE GLASS FABRICATION AND TESTING OF THE DWPF SLUDGE BATCH QUALIFICATION SAMPLE

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Amoroso, J.; Peeler, D.; Edwards, T.

    2012-05-11

    A recommendation to eliminate all characterization of pour stream glass samples and the glass fabrication and Product Consistency Test (PCT) of the sludge batch qualification sample was made by a Six-Sigma team chartered to eliminate non-value-added activities for the Defense Waste Processing Facility (DWPF) sludge batch qualification program and is documented in the report SS-PIP-2006-00030. That recommendation was supported through a technical data review by the Savannah River National Laboratory (SRNL) and is documented in the memorandums SRNL-PSE-2007-00079 and SRNL-PSE-2007-00080. At the time of writing those memorandums, the DWPF was processing sludge-only waste but, has since transitioned to a coupledmore » operation (sludge and salt). The SRNL was recently tasked to perform a similar data review relevant to coupled operations and re-evaluate the previous recommendations. This report evaluates the validity of eliminating the characterization of pour stream glass samples and the glass fabrication and Product Consistency Test (PCT) of the sludge batch qualification samples based on sludge-only and coupled operations. The pour stream sample has confirmed the DWPF's ability to produce an acceptable waste form from Slurry Mix Evaporator (SME) blending and product composition/durability predictions for the previous sixteen years but, ultimately the pour stream analysis has added minimal value to the DWPF's waste qualification strategy. Similarly, the information gained from the glass fabrication and PCT of the sludge batch qualification sample was determined to add minimal value to the waste qualification strategy since that sample is routinely not representative of the waste composition ultimately processed at the DWPF due to blending and salt processing considerations. Moreover, the qualification process has repeatedly confirmed minimal differences in glass behavior from actual radioactive waste to glasses fabricated from simulants or batch chemicals. In

  6. The effect of preparation strategies, qualification and professional background on clinical nurse educator confidence.

    PubMed

    Nguyen, Van N B; Forbes, Helen; Mohebbi, Mohammadreza; Duke, Maxine

    2018-06-12

    To describe how clinical nurse educators in Vietnam are prepared for their role; to identify which preparation strategies assist development of confidence in clinical teaching; and to measure the effect of educational qualifications and professional background on perceived confidence levels. The quality of clinical teaching can directly affect the quality of the student learning experience. The role of the clinical educator is complex and dynamic and requires a period of adjustment for successful role transition to occur. Planned orientation and specific preparation programs assist transition and reduce anxiety for new clinical nurse educators. There is, however, a lack of clear evidence to identify the form this preparation should take or which strategies are likely to facilitate the development of role confidence. Descriptive survey study. Cross-sectional surveys were used to collect data from 334 clinical nurse educators during January - March 2015. Eight preparation methods commonly used in Vietnam were identified. There was a small yet significant association between preparation and clinical nurse educators' perceived confidence. Formal preparation methods, as well as postgraduate qualifications and years of clinical teaching experience were linked to increased confidence in clinical teaching. Conversely, informal mentorship was found to hinder confidence development. This study identifies several preparation strategies that significantly enhance clinical educator confidence and readiness for their complex role. These preparation strategies drawn from the Vietnamese context, provide important examples for the wider nursing community to consider. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  7. SLUDGE BATCH 7B QUALIFICATION ACTIVITIES WITH SRS TANK FARM SLUDGE

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pareizs, J.; Click, D.; Lambert, D.

    2011-11-16

    Waste Solidification Engineering (WSE) has requested that characterization and a radioactive demonstration of the next batch of sludge slurry - Sludge Batch 7b (SB7b) - be completed in the Shielded Cells Facility of the Savannah River National Laboratory (SRNL) via a Technical Task Request (TTR). This characterization and demonstration, or sludge batch qualification process, is required prior to transfer of the sludge from Tank 51 to the Defense Waste Processing Facility (DWPF) feed tank (Tank 40). The current WSE practice is to prepare sludge batches in Tank 51 by transferring sludge from other tanks. Discharges of nuclear materials from Hmore » Canyon are often added to Tank 51 during sludge batch preparation. The sludge is washed and transferred to Tank 40, the current DWPF feed tank. Prior to transfer of Tank 51 to Tank 40, SRNL typically simulates the Tank Farm and DWPF processes with a Tank 51 sample (referred to as the qualification sample). With the tight schedule constraints for SB7b and the potential need for caustic addition to allow for an acceptable glass processing window, the qualification for SB7b was approached differently than past batches. For SB7b, SRNL prepared a Tank 51 and a Tank 40 sample for qualification. SRNL did not receive the qualification sample from Tank 51 nor did it simulate all of the Tank Farm washing and decanting operations. Instead, SRNL prepared a Tank 51 SB7b sample from samples of Tank 7 and Tank 51, along with a wash solution to adjust the supernatant composition to the final SB7b Tank 51 Tank Farm projections. SRNL then prepared a sample to represent SB7b in Tank 40 by combining portions of the SRNL-prepared Tank 51 SB7b sample and a Tank 40 Sludge Batch 7a (SB7a) sample. The blended sample was 71% Tank 40 (SB7a) and 29% Tank 7/Tank 51 on an insoluble solids basis. This sample is referred to as the SB7b Qualification Sample. The blend represented the highest projected Tank 40 heel (as of May 25, 2011), and thus, the

  8. Characterization of the SRNL-Washed tank 51 sludge batch 9 qualification sample

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pareizs, J. M.

    2016-01-01

    Savannah River National Laboratory (SRNL) personnel have been requested to qualify the next sludge batch (Sludge Batch 9 – SB9) for processing at the Defense Waste Processing Facility (DWPF). To accomplish this task, Savannah River Remediation (SRR) sent SRNL a 3-L sample of Tank 51H slurry to be characterized, washed, and then used in a lab-scale demonstration of the DWPF flowsheet (after combining with Tank 40H sludge). SRNL has washed the Tank 51H sample per the Tank Farm washing strategy as of October 20, 2015. A part of the qualification process is extensive radionuclide and chemical characterization of the SRNL-washedmore » Tank 51H slurry. This report documents the chemical characterization of the washed slurry; radiological characterization is in progress and will be documented in a separate report. The analytical results of this characterization are comparable to the Tank Farm projections. Therefore, it is recommended that SRNL use this washed slurry for the ongoing SB9 qualification activities.« less

  9. Characterization Of The As-Received Sludge Batch 9 Qualification Sample (Htf-51-15-81)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pareizs, J.

    Savannah River National Laboratory (SRNL) personnel have been requested to qualify the next sludge batch (Sludge Batch 9 – SB9) for processing at the Defense Waste Processing Facility (DWPF). To accomplish this task, Savannah River Remediation (SRR) has sent SRNL a 3-L slurried sample of Tank 51H (HTF-51-15-81) to be characterized, washed, and then used in a lab-scale demonstration of the DWPF flowsheet (potentially after combining with Tank 40H sludge). This report documents the first steps of the qualification process – characterization of the as-received Tank 51H qualification sample. These results will be used to support a reprojection of SB9more » by SRR from which final Tank 51H washing, frit development, and Chemical Processing Cell (CPC) activities will be based.« less

  10. Verification Of The Defense Waste Processing Facility's (DWPF) Process Digestion Methods For The Sludge Batch 8 Qualification Sample

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Click, D. R.; Edwards, T. B.; Wiedenman, B. J.

    2013-03-18

    This report contains the results and comparison of data generated from inductively coupled plasma – atomic emission spectroscopy (ICP-AES) analysis of Aqua Regia (AR), Sodium Peroxide/Sodium Hydroxide Fusion Dissolution (PF) and Cold Chem (CC) method digestions and Cold Vapor Atomic Absorption analysis of Hg digestions from the DWPF Hg digestion method of Sludge Batch 8 (SB8) Sludge Receipt and Adjustment Tank (SRAT) Receipt and SB8 SRAT Product samples. The SB8 SRAT Receipt and SB8 SRAT Product samples were prepared in the SRNL Shielded Cells, and the SRAT Receipt material is representative of the sludge that constitutes the SB8 Batch ormore » qualification composition. This is the sludge in Tank 51 that is to be transferred into Tank 40, which will contain the heel of Sludge Batch 7b (SB7b), to form the SB8 Blend composition.« less

  11. Tank 30 and 37 Supernatant Sample Cross-Check and Evaporator Feed Qualification Analysis-2012

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Oji, L. N.

    2013-03-07

    This report summarizes the analytical data reported by the F/H and Savannah River National Laboratories for the 2012 cross-check analysis for high level waste supernatant liquid samples from SRS Tanks 30 and 37. The intent of this Tank 30 and 37 sample analyses was to perform cross-checks against routine F/H Laboratory analyses (corrosion and evaporator feed qualification programs) using samples collected at the same time from both tanks as well as split samples from the tanks.

  12. Guidance on validation and qualification of processes and operations involving radiopharmaceuticals.

    PubMed

    Todde, S; Peitl, P Kolenc; Elsinga, P; Koziorowski, J; Ferrari, V; Ocak, E M; Hjelstuen, O; Patt, M; Mindt, T L; Behe, M

    2017-01-01

    Validation and qualification activities are nowadays an integral part of the day by day routine work in a radiopharmacy. This document is meant as an Appendix of Part B of the EANM "Guidelines on Good Radiopharmacy Practice (GRPP)" issued by the Radiopharmacy Committee of the EANM, covering the qualification and validation aspects related to the small-scale "in house" preparation of radiopharmaceuticals. The aim is to provide more detailed and practice-oriented guidance to those who are involved in the small-scale preparation of radiopharmaceuticals which are not intended for commercial purposes or distribution. The present guideline covers the validation and qualification activities following the well-known "validation chain", that begins with editing the general Validation Master Plan document, includes all the required documentation (e.g. User Requirement Specification, Qualification protocols, etc.), and leads to the qualification of the equipment used in the preparation and quality control of radiopharmaceuticals, until the final step of Process Validation. A specific guidance to the qualification and validation activities specifically addressed to small-scale hospital/academia radiopharmacies is here provided. Additional information, including practical examples, are also available.

  13. Science teacher improvement: A study of the change in preparation and qualifications of public middle school science teachers

    NASA Astrophysics Data System (ADS)

    Wickler, Nicole I. Z.

    According to the National Commission on Teaching and America's Future (1996), a teacher's professional preparation, their work conditions and sense of efficacy are fundamental to improving elementary and secondary education. These factors lie at the core of educational reforms that seek to raise standards, reshape curricula, and restructure the way schools operate. The call to reconceptualize the practice of teaching and the interaction between teachers and students ring hollow without a careful examination of actions that have taken place in the workplace of teachers themselves. A national profile that identifies key characteristics of the current status of public middle school science teachers preparation, teaching qualifications, and work environments can provide a context for better understanding the current conditions that confront science teachers. This study seeks to provide critical information in four major areas: (1) preservice learning and teaching assignment; (2) continued learning; (3) supportive work environment, and (4) teachers' sense of efficacy. This study is based on current efforts by the National Center for Education Statistics (LACES) to collect data of key indicators of teacher preparation and qualifications using a large-scale survey administered to a nationally representative sample of full-time public school teachers whose primary teaching assignment is in science. In this effort, the information reported in this study utilizes the NCES's Schools and Staffing Surveys (SASS) from 1987--88 and 1993--94. Significant change between 1987--77 and 1993--94 was determined using a t-test for independent means. In addition, frequency counts were analyzed using a chi-square statistic to determine if more "qualified middle school science teachers" were located in particular schools by urbanicity location or/and percent minority enrollment. In general, the quality of middle school science teachers across the country is declining. Teachers report they

  14. Results Of Initial Analyses Of The Salt (Macro) Batch 9 Tank 21H Qualification Samples

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Peters, T.

    2015-10-08

    Savannah River National Laboratory (SRNL) analyzed samples from Tank 21H in support of qualification of Interim Salt Disposition Project (ISDP) Salt (Macro) Batch 9 for processing through the Actinide Removal Process (ARP) and the Modular Caustic-Side Solvent Extraction Unit (MCU). This document reports the initial results of the analyses of samples of Tank 21H. Analysis of the Tank 21H Salt (Macro) Batch 9 composite sample indicates that the material does not display any unusual characteristics. Further results on the chemistry and other tests will be issued in the future.

  15. Results from the interim salt disposition program macrobatch 10 tank 21H qualification samples

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Peters, T. B.; Bannochie, C. J.

    2017-02-23

    Savannah River National Laboratory (SRNL) analyzed samples from Tank 21H in support of qualification of Macrobatch (Salt Batch) 10 for the Interim Salt Disposition Program (ISDP). This document reports characterization data on the samples of Tank 21H and fulfills the requirements of Deliverable 3 of the Technical Task Request (TTR). Further work will report the results of the Extraction-Scrub-Strip (ESS) testing (Task 5 of the TTR) using the Tank 21H material. Task 4 of the TTR (MST Strike) will not be completed for Salt Batch 10.

  16. Sampling hazelnuts for aflatoxin: uncertainty associated with sampling, sample preparation, and analysis.

    PubMed

    Ozay, Guner; Seyhan, Ferda; Yilmaz, Aysun; Whitaker, Thomas B; Slate, Andrew B; Giesbrecht, Francis

    2006-01-01

    The variability associated with the aflatoxin test procedure used to estimate aflatoxin levels in bulk shipments of hazelnuts was investigated. Sixteen 10 kg samples of shelled hazelnuts were taken from each of 20 lots that were suspected of aflatoxin contamination. The total variance associated with testing shelled hazelnuts was estimated and partitioned into sampling, sample preparation, and analytical variance components. Each variance component increased as aflatoxin concentration (either B1 or total) increased. With the use of regression analysis, mathematical expressions were developed to model the relationship between aflatoxin concentration and the total, sampling, sample preparation, and analytical variances. The expressions for these relationships were used to estimate the variance for any sample size, subsample size, and number of analyses for a specific aflatoxin concentration. The sampling, sample preparation, and analytical variances associated with estimating aflatoxin in a hazelnut lot at a total aflatoxin level of 10 ng/g and using a 10 kg sample, a 50 g subsample, dry comminution with a Robot Coupe mill, and a high-performance liquid chromatographic analytical method are 174.40, 0.74, and 0.27, respectively. The sampling, sample preparation, and analytical steps of the aflatoxin test procedure accounted for 99.4, 0.4, and 0.2% of the total variability, respectively.

  17. WTP Waste Feed Qualification: Glass Fabrication Unit Operation Testing Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stone, M. E.; Newell, J. D.; Johnson, F. C.

    The waste feed qualification program is being developed to protect the Hanford Tank Waste Treatment and Immobilization Plant (WTP) design, safety basis, and technical basis by assuring waste acceptance requirements are met for each staged waste feed campaign prior to transfer from the Tank Operations Contractor to the feed receipt vessels inside the Pretreatment Facility. The Waste Feed Qualification Program Plan describes the three components of waste feed qualification: 1. Demonstrate compliance with the waste acceptance criteria 2. Determine waste processability 3. Test unit operations at laboratory scale. The glass fabrication unit operation is the final step in the processmore » demonstration portion of the waste feed qualification process. This unit operation generally consists of combining each of the waste feed streams (high-level waste (HLW) and low-activity waste (LAW)) with Glass Forming Chemicals (GFCs), fabricating glass coupons, performing chemical composition analysis before and after glass fabrication, measuring hydrogen generation rate either before or after glass former addition, measuring rheological properties before and after glass former addition, and visual observation of the resulting glass coupons. Critical aspects of this unit operation are mixing and sampling of the waste and melter feeds to ensure representative samples are obtained as well as ensuring the fabrication process for the glass coupon is adequate. Testing was performed using a range of simulants (LAW and HLW simulants), and these simulants were mixed with high and low bounding amounts of GFCs to evaluate the mixing, sampling, and glass preparation steps in shielded cells using laboratory techniques. The tests were performed with off-the-shelf equipment at the Savannah River National Laboratory (SRNL) that is similar to equipment used in the SRNL work during qualification of waste feed for the Defense Waste Processing Facility (DWPF) and other waste treatment facilities at

  18. 42 CFR 493.1483 - Standard: Cytotechnologist qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... person examining cytology slide preparations must meet the qualifications of § 493.1449 (b) or (k), or... laboratory directed by a pathologist or other physician providing cytology services; and (iii) Completed 2..., have full-time experience of at least 2 years or equivalent examining cytology slide preparations...

  19. 42 CFR 493.1483 - Standard: Cytotechnologist qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... person examining cytology slide preparations must meet the qualifications of § 493.1449 (b) or (k), or... laboratory directed by a pathologist or other physician providing cytology services; and (iii) Completed 2..., have full-time experience of at least 2 years or equivalent examining cytology slide preparations...

  20. 42 CFR 493.1483 - Standard: Cytotechnologist qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... person examining cytology slide preparations must meet the qualifications of § 493.1449 (b) or (k), or... laboratory directed by a pathologist or other physician providing cytology services; and (iii) Completed 2..., have full-time experience of at least 2 years or equivalent examining cytology slide preparations...

  1. 42 CFR 493.1483 - Standard: Cytotechnologist qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... person examining cytology slide preparations must meet the qualifications of § 493.1449 (b) or (k), or... laboratory directed by a pathologist or other physician providing cytology services; and (iii) Completed 2..., have full-time experience of at least 2 years or equivalent examining cytology slide preparations...

  2. 42 CFR 493.1483 - Standard: Cytotechnologist qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... person examining cytology slide preparations must meet the qualifications of § 493.1449 (b) or (k), or... laboratory directed by a pathologist or other physician providing cytology services; and (iii) Completed 2..., have full-time experience of at least 2 years or equivalent examining cytology slide preparations...

  3. 16 CFR 1209.34 - Qualification testing.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....34 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS INTERIM SAFETY STANDARD FOR CELLULOSE INSULATION Certification § 1209.34 Qualification testing. (a... labelers, or importers may select samples for qualification testing of a product in any manner they desire. ...

  4. Expedition 50 Qualification Exams

    NASA Image and Video Library

    2016-10-25

    Expedition 50 crew members NASA astronaut Peggy Whitson, left, Russian cosmonaut Oleg Novitskiy of Roscosmos, center, and ESA astronaut Thomas Pesquet laugh together as they prepare for their final qualification exams, Tuesday, Oct. 25, 2016, at the Gagarin Cosmonaut Training Center (GCTC) in Star City, Russia. Photo Credit: (NASA/Bill Ingalls)

  5. Sample results from the integrated salt disposition program macrobatch 6 tank 21H qualifications MST solids sample

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Peters, T. B.

    2013-02-26

    Savannah River National Laboratory (SRNL) performed experiments on qualification material for use in the Integrated Salt Disposition Program (ISDP) Batch 6 processing. As part of this qualification work, SRNL performed an Actinide Removal Process (ARP) test. From this test, the residual monosodium titanate (MST) was analyzed for radionuclide uptake. The results of these analyses are reported and are within historical precedent.

  6. Extraction-Scrub-Strip test results from the interim Salt Disposition Program Macrobatch 9 Tank 21H qualification samples

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Peters, T.

    2016-02-23

    Savannah River National Laboratory (SRNL) analyzed samples from Tank 21H in support of qualification of Macrobatch (Salt Batch) 9 for the Interim Salt Disposition Program (ISDP). The Salt Batch 9 characterization results were previously reported. An Extraction-Scrub-Strip (ESS) test was performed to determine cesium distribution ratios (D (Cs)) and cesium concentration in the strip effluent and decontaminated salt solution (DSS) streams; this data will be used by Tank Farm Engineering to project a cesium decontamination factor (DF). This test used actual Tank 21H material, and a blend solvent prepared by SRNL that mimics the solvent composition currently being used atmore » the Modular Caustic-Side Solvent Extraction Unit (MCU). The ESS test showed acceptable performance with an extraction D (Cs) value of 52.4. This value is consistent with results from previous salt batch ESS tests using similar solvent formulations. This compares well against the predicted value of 56.5 from a recently created D (Cs) model« less

  7. Synchrotron/crystal sample preparation

    NASA Technical Reports Server (NTRS)

    Johnson, R. Barry

    1993-01-01

    The Center for Applied Optics (CAO) of the University of Alabama in Huntsville (UAH) prepared this final report entitled 'Synchrotron/Crystal Sample Preparation' in completion of contract NAS8-38609, Delivery Order No. 53. Hughes Danbury Optical Systems (HDOS) is manufacturing the Advanced X-ray Astrophysics Facility (AXAF) mirrors. These thin-walled, grazing incidence, Wolter Type-1 mirrors, varying in diameter from 1.2 to 0.68 meters, must be ground and polished using state-of-the-art techniques in order to prevent undue stress due to damage or the presence of crystals and inclusions. The effect of crystals on the polishing and grinding process must also be understood. This involves coating special samples of Zerodur and measuring the reflectivity of the coatings in a synchrotron system. In order to gain the understanding needed on the effect of the Zerodur crystals by the grinding and polishing process, UAH prepared glass samples by cutting, grinding, etching, and polishing as required to meet specifications for witness bars for synchrotron measurements and for investigations of crystals embedded in Zerodur. UAH then characterized these samples for subsurface damage and surface roughness and figure.

  8. Results of initial analyses of the salt (macro) batch 10 tank 21H qualification samples

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Peters, T. B.

    2017-01-01

    Savannah River National Laboratory (SRNL) analyzed samples from Tank 21H in support of qualification of Interim Salt Disposition Project (ISDP) Salt (Macro) Batch 10 for processing through the Actinide Removal Process (ARP) and the Modular Caustic-Side Solvent Extraction Unit (MCU). This document reports the initial results of the analyses of samples of Tank 21H. Analysis of the Tank 21H Salt (Macro) Batch 10 composite sample indicates that the material does not display any unusual characteristics or observations, such as floating solids, the presence of large amount of solids, or unusual colors. Further sample results will be reported in a futuremore » document. This memo satisfies part of Deliverable 3 of the Technical Task Request (TTR).« less

  9. Results of initial analyses of the salt (macro) batch 11 Tank 21H qualification samples

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Peters, T. B.

    Savannah River National Laboratory (SRNL) analyzed samples from Tank 21H in support of qualification of Interim Salt Disposition Project (ISDP) Salt (Macro) Batch 11 for processing through the Actinide Removal Process (ARP) and the Modular Caustic-Side Solvent Extraction Unit (MCU). This document reports the initial results of the analyses of samples of Tank 21H. Analysis of the Tank 21H Salt (Macro) Batch 11 composite sample indicates that the material does not display any unusual characteristics or observations, such as floating solids, the presence of large amounts of solids, or unusual colors. Further sample results will be reported in a futuremore » document. This memo satisfies part of Deliverable 3 of the Technical Task Request (TTR).« less

  10. Global National Qualifications Framework Inventory

    ERIC Educational Resources Information Center

    Bjornavold, Jens; Pevec-Grm, Slava; Graham, Michael; Deij, Arjen; Singh, Madhu; Charkoun, Borhène; Agrawal, Shivani

    2013-01-01

    This publication is a global, country-by-country, inventory of National Qualifications Frameworks (NQFs). It is a copublication, prepared by two EU agencies, the European Training Foundation (ETF) and the Centre for the Development of Vocational Training (Cedefop); and UNESCO's Institute for Lifelong Learning (UIL) and the Section for TVET at…

  11. Results of initial analyses of the salt (macro) batch 9 tank 21H qualification samples

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Peters, T. B.

    2015-10-01

    Savannah River National Laboratory (SRNL) analyzed samples from Tank 21H in support of qualification of Interim Salt Disposition Project (ISDP) Salt (Macro) Batch 9 for processing through the Actinide Removal Process (ARP) and the Modular Caustic-Side Solvent Extraction Unit (MCU). This document reports the initial results of the analyses of samples of Tank 21H. Analysis of the Tank 21H Salt (Macro) Batch 9 composite sample indicates that the material does not display any unusual characteristics or observations, such as floating solids, the presence of large amount of solids, or unusual colors. Further results on the chemistry and other tests willmore » be issued in the future.« less

  12. [Sample preparation and bioanalysis in mass spectrometry].

    PubMed

    Bourgogne, Emmanuel; Wagner, Michel

    2015-01-01

    The quantitative analysis of compounds of clinical interest of low molecular weight (<1000 Da) in biological fluids is currently in most cases performed by liquid chromatography-mass spectrometry (LC-MS). Analysis of these compounds in biological fluids (plasma, urine, saliva, hair...) is a difficult task requiring a sample preparation. Sample preparation is a crucial part of chemical/biological analysis and in a sense is considered the bottleneck of the whole analytical process. The main objectives of sample preparation are the removal of potential interferences, analyte preconcentration, and converting (if needed) the analyte into a more suitable form for detection or separation. Without chromatographic separation, endogenous compounds, co-eluted products may affect a quantitative method in mass spectrometry performance. This work focuses on three distinct parts. First, quantitative bioanalysis will be defined, different matrices and sample preparation techniques currently used in bioanalysis by mass spectrometry of/for small molecules of clinical interest in biological fluids. In a second step the goals of sample preparation will be described. Finally, in a third step, sample preparation strategies will be made either directly ("dilute and shoot") or after precipitation.

  13. Graduates from dual qualification courses, registered nurse and health visitor: a career history study.

    PubMed

    Drennan, Vari M; Porter, Elizabeth M J; Grant, Robert L

    2013-08-01

    Educationalists and managers internationally are challenged to find ways of preparing, recruiting early in their careers, and retaining nurses into public health roles in primary care. Public health nursing qualifications are post-initial nurse registration in the United Kingdom as in some other countries. In the mid twentieth century there were a number of innovative programmes of dual qualification: registered nurse and health visitor (the United Kingdom term for public health nurse). To investigate the career histories of graduates from courses integrating both nursing and health visitor qualifications. An observational, survey study. The United Kingdom. A purposive sample of graduates from integrated registered nurse and health visitor programmes, 1959-1995, from one University. Self completed, anonymous, survey sent to graduates, with contact details known to the University and through snowballing techniques, in 2011. Forty five women (56%), graduates in all four decades, returned the survey. A significant majority (82%) had taken up health visitor posts on completing the course. Over their careers, 42% of all jobs held were as health visitors. Only four never worked in a post that required a health visiting qualification. Most had undertaken paid work throughout their careers that focused on aspects of public health, often linked to child, maternal and/or family wellbeing. Many held teaching/lecturing and management posts at some point in their career. Those holding management posts were more likely to report leaving them as a result of organisational re-structuring or redundancy than those in non-management posts. Courses that prepare students to be both nurses and health visitors result in a majority of graduates who take up posts as health visitors on qualification and subsequently. Nurse education planners may find this evidence of value in determining ways of providing a future workforce for public health nursing. Copyright © 2012 Elsevier Ltd. All

  14. Microfluidic Sample Preparation for Diagnostic Cytopathology

    PubMed Central

    Mach, Albert J.; Adeyiga, Oladunni B.; Di Carlo, Dino

    2014-01-01

    The cellular components of body fluids are routinely analyzed to identify disease and treatment approaches. While significant focus has been placed on developing cell analysis technologies, tools to automate the preparation of cellular specimens have been more limited, especially for body fluids beyond blood. Preparation steps include separating, concentrating, and exposing cells to reagents. Sample preparation continues to be routinely performed off-chip by technicians, preventing cell-based point-of-care diagnostics, increasing the cost of tests, and reducing the consistency of the final analysis following multiple manually-performed steps. Here, we review the assortment of biofluids for which suspended cells are analyzed, along with their characteristics and diagnostic value. We present an overview of the conventional sample preparation processes for cytological diagnosis. We finally discuss the challenges and opportunities in developing microfluidic devices for the purpose of automating or miniaturizing these processes, with particular emphases on preparing large or small volume samples, working with samples of high cellularity, automating multi-step processes, and obtaining high purity subpopulations of cells. We hope to convey the importance of and help identify new research directions addressing the vast biological and clinical applications in preparing and analyzing the array of available biological fluids. Successfully addressing the challenges described in this review can lead to inexpensive systems to improve diagnostic accuracy while simultaneously reducing overall systemic healthcare costs. PMID:23380972

  15. Vocational Education Qualifications' Roles in Pathways to Work in Liberal Market Economies

    ERIC Educational Resources Information Center

    Wheelahan, Leesa; Moodie, Gavin

    2017-01-01

    The links between vocational qualifications and occupational destinations are weak in many Anglophone countries, even though the explicit purpose of vocational qualifications is to prepare individuals for occupations. Using Australia and Canada as case studies, this is explained at three levels of analysis: at the national level by systems of…

  16. Novel Sample-handling Approach for XRD Analysis with Minimal Sample Preparation

    NASA Technical Reports Server (NTRS)

    Sarrazin, P.; Chipera, S.; Bish, D.; Blake, D.; Feldman, S.; Vaniman, D.; Bryson, C.

    2004-01-01

    Sample preparation and sample handling are among the most critical operations associated with X-ray diffraction (XRD) analysis. These operations require attention in a laboratory environment, but they become a major constraint in the deployment of XRD instruments for robotic planetary exploration. We are developing a novel sample handling system that dramatically relaxes the constraints on sample preparation by allowing characterization of coarse-grained material that would normally be impossible to analyze with conventional powder-XRD techniques.

  17. The Future of Australian Vocational Education Qualifications Depends on a New Social Settlement

    ERIC Educational Resources Information Center

    Wheelahan, Leesa

    2015-01-01

    This article argues that the current social settlement underpinning vocational education and training (VET) in Australia is fractured. The current settlement is low trust and consists of qualifications based on competency-based training models of curriculum and competitive markets. The result is narrow qualifications that do not prepare people for…

  18. 40 CFR 761.323 - Sample preparation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 761.323 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) TOXIC SUBSTANCES CONTROL... Remediation Waste Samples § 761.323 Sample preparation. (a) The comparison study requires analysis of a minimum of 10 samples weighing at least 300 grams each. Samples of PCB remediation waste used in the...

  19. 40 CFR 761.323 - Sample preparation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 761.323 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) TOXIC SUBSTANCES CONTROL... Remediation Waste Samples § 761.323 Sample preparation. (a) The comparison study requires analysis of a minimum of 10 samples weighing at least 300 grams each. Samples of PCB remediation waste used in the...

  20. 40 CFR 761.323 - Sample preparation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 761.323 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) TOXIC SUBSTANCES CONTROL... Remediation Waste Samples § 761.323 Sample preparation. (a) The comparison study requires analysis of a minimum of 10 samples weighing at least 300 grams each. Samples of PCB remediation waste used in the...

  1. Changing Patterns in Vocational Entry Qualifications, Student Support and Outcomes in Undergraduate Degree Programmes

    ERIC Educational Resources Information Center

    Shields, Robin; Masardo, Alex

    2015-01-01

    This study investigates the differences in degree attainment between students entering higher education through vocational qualification pathways and students entering through traditional A-level routes. The report also analyses how well students with vocational qualifications are prepared for and supported in their studies at higher education.…

  2. 7 CFR 27.21 - Preparation of samples of cotton.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Preparation of samples of cotton. 27.21 Section 27.21... REGULATIONS COTTON CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Inspection and Samples § 27.21 Preparation of samples of cotton. The samples from each bale shall be prepared as specified in this section...

  3. 7 CFR 27.21 - Preparation of samples of cotton.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Preparation of samples of cotton. 27.21 Section 27.21... REGULATIONS COTTON CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Inspection and Samples § 27.21 Preparation of samples of cotton. The samples from each bale shall be prepared as specified in this section...

  4. 7 CFR 27.21 - Preparation of samples of cotton.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Preparation of samples of cotton. 27.21 Section 27.21... REGULATIONS COTTON CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Inspection and Samples § 27.21 Preparation of samples of cotton. The samples from each bale shall be prepared as specified in this section...

  5. Recent advances in applications of nanomaterials for sample preparation.

    PubMed

    Xu, Linnan; Qi, Xiaoyue; Li, Xianjiang; Bai, Yu; Liu, Huwei

    2016-01-01

    Sample preparation is a key step for qualitative and quantitative analysis of trace analytes in complicated matrix. Along with the rapid development of nanotechnology in material science, numerous nanomaterials have been developed with particularly useful applications in analytical chemistry. Benefitting from their high specific areas, increased surface activities, and unprecedented physical/chemical properties, the potentials of nanomaterials for rapid and efficient sample preparation have been exploited extensively. In this review, recent progress of novel nanomaterials applied in sample preparation has been summarized and discussed. Both nanoparticles and nanoporous materials are evaluated for their unusual performance in sample preparation. Various compositions and functionalizations extended the applications of nanomaterials in sample preparations, and distinct size and shape selectivity was generated from the diversified pore structures of nanoporous materials. Such great variety make nanomaterials a kind of versatile tools in sample preparation for almost all categories of analytes. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. Extending the solvent-free MALDI sample preparation method.

    PubMed

    Hanton, Scott D; Parees, David M

    2005-01-01

    Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is an important technique to characterize many different materials, including synthetic polymers. MALDI mass spectral data can be used to determine the polymer average molecular weights, repeat units, and end groups. One of the key issues in traditional MALDI sample preparation is making good solutions of the analyte and the matrix. Solvent-free sample preparation methods have been developed to address these issues. Previous results of solvent-free or dry prepared samples show some advantages over traditional wet sample preparation methods. Although the results of the published solvent-free sample preparation methods produced excellent mass spectra, we found the method to be very time-consuming, with significant tool cleaning, which presents a significant possibility of cross contamination. To address these issues, we developed an extension of the solvent-free method that replaces the mortar and pestle grinding with ball milling the sample in a glass vial with two small steel balls. This new method generates mass spectra with equal quality of the previous methods, but has significant advantages in productivity, eliminates cross contamination, and is applicable to liquid and soft or waxy analytes.

  7. 40 CFR 761.323 - Sample preparation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Remediation Waste Samples § 761.323 Sample preparation. (a) The comparison study requires analysis of a minimum of 10 samples weighing at least 300 grams each. Samples of PCB remediation waste used in the... PCB remediation waste at the cleanup site, or must be the same kind of material as that waste. For...

  8. SLUDGE WASHING AND DEMONSTRATION OF THE DWPF FLOWSHEET IN THE SRNL SHIELDED CELLS FOR SLUDGE BATCH 7A QUALIFICATION

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pareizs, J.; Billings, A.; Click, D.

    2011-07-08

    Waste Solidification Engineering (WSE) has requested that characterization and a radioactive demonstration of the next batch of sludge slurry (Sludge Batch 7a*) be completed in the Shielded Cells Facility of the Savannah River National Laboratory (SRNL) via a Technical Task Request (TTR). This characterization and demonstration, or sludge batch qualification process, is required prior to transfer of the sludge from Tank 51 to the Defense Waste Processing Facility (DWPF) feed tank (Tank 40). The current WSE practice is to prepare sludge batches in Tank 51 by transferring sludge from other tanks. Discharges of nuclear materials from H Canyon are oftenmore » added to Tank 51 during sludge batch preparation. The sludge is washed and transferred to Tank 40, the current DWPF feed tank. Prior to transfer of Tank 51 to Tank 40, SRNL simulates the Tank Farm and DWPF processes with a Tank 51 sample (referred to as the qualification sample). Sludge Batch 7a (SB7a) is composed of portions of Tanks 4, 7, and 12; the Sludge Batch 6 heel in Tank 51; and a plutonium stream from H Canyon. SRNL received the Tank 51 qualification sample (sample ID HTF-51-10-125) following sludge additions to Tank 51. This report documents: (1) The washing (addition of water to dilute the sludge supernate) and concentration (decanting of supernate) of the SB7a - Tank 51 qualification sample to adjust sodium content and weight percent insoluble solids to Tank Farm projections. (2) The performance of a DWPF Chemical Process Cell (CPC) simulation using the washed Tank 51 sample. The simulation included a Sludge Receipt and Adjustment Tank (SRAT) cycle, where acid was added to the sludge to destroy nitrite and reduce mercury, and a Slurry Mix Evaporator (SME) cycle, where glass frit was added to the sludge in preparation for vitrification. The SME cycle also included replication of five canister decontamination additions and concentrations. Processing parameters were based on work with a non

  9. Modular microfluidic system for biological sample preparation

    DOEpatents

    Rose, Klint A.; Mariella, Jr., Raymond P.; Bailey, Christopher G.; Ness, Kevin Dean

    2015-09-29

    A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis virus filter module, d) an isotachophoresis nucleic acid filter module, e) a lyses module, and f) an isotachophoresis-based nucleic acid filter.

  10. Recent advances of mesoporous materials in sample preparation.

    PubMed

    Zhao, Liang; Qin, Hongqiang; Wu, Ren'an; Zou, Hanfa

    2012-03-09

    Sample preparation has been playing an important role in the analysis of complex samples. Mesoporous materials as the promising adsorbents have gained increasing research interest in sample preparation due to their desirable characteristics of high surface area, large pore volume, tunable mesoporous channels with well defined pore-size distribution, controllable wall composition, as well as modifiable surface properties. The aim of this paper is to review the recent advances of mesoporous materials in sample preparation with emphases on extraction of metal ions, adsorption of organic compounds, size selective enrichment of peptides/proteins, specific capture of post-translational peptides/proteins and enzymatic reactor for protein digestion. Copyright © 2011 Elsevier B.V. All rights reserved.

  11. Analytical Overview of the European and Russian Qualifications Frameworks with a Focus on Doctoral Degree Level

    ERIC Educational Resources Information Center

    Chigisheva, Oksana; Bondarenko, Anna; Soltovets, Elena

    2017-01-01

    The paper provides analytical insights into highly acute issues concerning preparation and adoption of Qualifications Frameworks being an adequate response to the growing interactions at the global labor market and flourishing of knowledge economy. Special attention is paid to the analyses of transnational Meta Qualifications Frameworks (A…

  12. 40 CFR 761.323 - Sample preparation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PROHIBITIONS Self-Implementing Alternative Extraction and Chemical Analysis Procedures for Non-liquid PCB Remediation Waste Samples § 761.323 Sample preparation. (a) The comparison study requires analysis of a... of use in this chemical extraction and chemical analysis comparison study, a person may adjust PCB...

  13. JOB BRIEFS, SELECTED FEDERAL JOBS--DUTIES, QUALIFICATION REQUIREMENTS, SAMPLE TEST QUESTIONS.

    ERIC Educational Resources Information Center

    Civil Service Commission, Washington, DC.

    INFORMATION ABOUT JOBS IN THE FEDERAL GOVERNMENT FOR PERSONS WITH LIMITED WORK EXPERIENCE OR EDUCATION IS PROVIDED. JOB BRIEFS DESCRIBING THE WORK, OPPORTUNITIES, NUMBER EMPLOYED, EMPLOYING AGENCIES, AND QUALIFICATIONS ARE GIVEN FOR (1) 19 WHITE COLLAR AND POST OFFICE JOBS SUCH AS CLERK-TYPIST, CLERK-STENOGRAPHER, NURSING ASSISTANT, SOIL…

  14. Sample Preparation for Electron Probe Microanalysis—Pushing the Limits

    PubMed Central

    Geller, Joseph D.; Engle, Paul D.

    2002-01-01

    There are two fundamental considerations in preparing samples for electron probe microanalysis (EPMA). The first one may seem obvious, but we often find it is overlooked. That is, the sample analyzed should be representative of the population from which it comes. The second is a direct result of the assumptions in the calculations used to convert x-ray intensity ratios, between the sample and standard, to concentrations. Samples originate from a wide range of sources. During their journey to being excited under the electron beam for the production of x rays there are many possibilities for sample alteration. Handling can contaminate samples by adding extraneous matter. In preparation, the various abrasives used in sizing the sample by sawing, grinding and polishing can embed themselves. The most accurate composition of a contaminated sample is, at best, not representative of the original sample; it is misleading. Our laboratory performs EPMA analysis on customer submitted samples and prepares over 250 different calibration standards including pure elements, compounds, alloys, glasses and minerals. This large variety of samples does not lend itself to mass production techniques, including automatic polishing. Our manual preparation techniques are designed individually for each sample. The use of automated preparation equipment does not lend itself to this environment, and is not included in this manuscript. The final step in quantitative electron probe microanalysis is the conversion of x-ray intensities ratios, known as the “k-ratios,” to composition (in mass fraction or atomic percent) and/or film thickness. Of the many assumptions made in the ZAF (where these letters stand for atomic number, absorption and fluorescence) corrections the localized geometry between the sample and electron beam, or takeoff angle, must be accurately known. Small angular errors can lead to significant errors in the final results. The sample preparation technique then becomes very

  15. Results of qualification tests on water-level sensing instruments, 1987

    USGS Publications Warehouse

    Olive, T.E.

    1989-01-01

    The U.S. Geological Survey 's Hydrologic Instrumentation Facility at the Stennis Space Center, Mississippi, conducts qualification tests on water level sensing instruments. Instrument systems, which meet or exceed the Survey 's minimum performance requirements, are placed on the Survey 's Qualified Products List. The qualification tests conducted in 1987 added two instrument systems to the Survey 's Qualified Products List. One system met requirements for use at a daily-discharge station , and the other system met requirements for a special-case station. The report is prepared for users of hydrologic instruments. The report provides a list of instrument features, describes the instrument systems, summarizes test procedures, and presents test results for the two instrument systems that met the Survey 's minimum performance standards for the 1987 round of qualification tests. (USGS)

  16. Development of a qualification standard for adhesives used in hybrid microcircuits

    NASA Technical Reports Server (NTRS)

    Licari, J. J.; Weigand, B. L.; Soykin, C. A.

    1981-01-01

    Improved qualification standards and test procedures for adhesives used in microelectronic packaging are developed. The test methods in specification for the Selection and Use of Organic Adhesives in Hybrid Microcircuits are reevaluated versus industry and government requirements. Four electrically insulative and four electrically conductive adhesives used in the assembly of hybrid microcircuits are selected to evaluate the proposed revised test methods. An estimate of the cost to perform qualification testing of an adhesive to the requirements of the revised specification is also prepared.

  17. Data Qualification Report: Pore Water Data for Use on the Yucca Mountain Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    H. Miller; R. Monks; C. Warren

    Pore water data associated with Data Tracking Number (DTN) No.LL990702804244.100 are referenced in the Analysis and Model Reports (AMRs) prepared to support the Site Recommendation in determining the suitability of the Yucca Mountain, Nevada as a repository for high-level nuclear waste. It has been determined, in accordance with procedure AP-3.15Q Rev. 1, ICN 1, ''Managing Technical Product Inputs'', Attachment 6 , that the DTN-referenced data are used in AMRs that provide a direct calculation of ''Principal Factors'' for the Post-closure Safety Case or Potentially Disruptive Processes or Events. Therefore, in accordance with the requirements of procedure AP-SIII.2Q, Rev 0, ICNmore » 2, ''Qualification of Unqualified Data and the Documentation of Rationale for Accepted Data'', Section 5.3.1 .a, a Data Qualification Report has been prepared for submittal to the Assistant Manager, Office of Project Execution for concurrence. This report summarizes the findings of the Data Qualification Team assembled to evaluate unqualified ''pore water data'' represented by DTN No. LL990702804244.100. This DTN is currently used in the following AMRs: Drift-Scale Coupled Processes (DST and THC Seepage) Models (CRWMS M&O 2000a), Environment of the Surfaces of the Drip Shield and Waste Package Outer Barrier (CRWMS M&O 2000b), and Engineered Barrier System: Physical and Chemical Environment Model (CRWMS M&O 2000c). Mineral composition of pore water submitted to the Technical Data Management System (TDMS) using the subject DTN were acquired data from the analysis pore water samples sent to Lawrence Livermore National Laboratory's (LLNL) by UFA Ventures, Inc. and analyzed by LLNL's Analytical Sciences/Analytical and Nuclear Chemistry Division (ASD). The purpose and scope of the AMRs that reference the subject DTN and the potential application of pore water data is described below. These AMRs use only that data associated with the specific samples: ESF-HD-PERM-1, ESF-HD-PERM-2, and ESF

  18. 40 CFR 761.392 - Preparing validation study samples.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Preparing validation study samples..., AND USE PROHIBITIONS Comparison Study for Validating a New Performance-Based Decontamination Solvent Under § 761.79(d)(4) § 761.392 Preparing validation study samples. (a)(1) To validate a procedure to...

  19. 40 CFR 761.392 - Preparing validation study samples.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Preparing validation study samples..., AND USE PROHIBITIONS Comparison Study for Validating a New Performance-Based Decontamination Solvent Under § 761.79(d)(4) § 761.392 Preparing validation study samples. (a)(1) To validate a procedure to...

  20. Standardized Sample Preparation Using a Drop-on-Demand Printing Platform

    DTIC Science & Technology

    2013-05-07

    successful and robust methodology for energetic sample preparation. Keywords: drop-on-demand; inkjet printing; sample preparation OPEN ACCESS...on a similar length scale. Recently, drop-on-demand inkjet printing technology has emerged as an effective approach to produce test materials to...which most of the material is concentrated along the edges, samples prepared using drop-on-demand inkjet technology demonstrate excellent uniform

  1. [Recent advances in sample preparation methods of plant hormones].

    PubMed

    Wu, Qian; Wang, Lus; Wu, Dapeng; Duan, Chunfeng; Guan, Yafeng

    2014-04-01

    Plant hormones are a group of naturally occurring trace substances which play a crucial role in controlling the plant development, growth and environment response. With the development of the chromatography and mass spectroscopy technique, chromatographic analytical method has become a widely used way for plant hormone analysis. Among the steps of chromatographic analysis, sample preparation is undoubtedly the most vital one. Thus, a highly selective and efficient sample preparation method is critical for accurate identification and quantification of phytohormones. For the three major kinds of plant hormones including acidic plant hormones & basic plant hormones, brassinosteroids and plant polypeptides, the sample preparation methods are reviewed in sequence especially the recently developed methods. The review includes novel methods, devices, extractive materials and derivative reagents for sample preparation of phytohormones analysis. Especially, some related works of our group are included. At last, the future developments in this field are also prospected.

  2. Current trends in sample preparation for cosmetic analysis.

    PubMed

    Zhong, Zhixiong; Li, Gongke

    2017-01-01

    The widespread applications of cosmetics in modern life make their analysis particularly important from a safety point of view. There is a wide variety of restricted ingredients and prohibited substances that primarily influence the safety of cosmetics. Sample preparation for cosmetic analysis is a crucial step as the complex matrices may seriously interfere with the determination of target analytes. In this review, some new developments (2010-2016) in sample preparation techniques for cosmetic analysis, including liquid-phase microextraction, solid-phase microextraction, matrix solid-phase dispersion, pressurized liquid extraction, cloud point extraction, ultrasound-assisted extraction, and microwave digestion, are presented. Furthermore, the research and progress in sample preparation techniques and their applications in the separation and purification of allowed ingredients and prohibited substances are reviewed. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. The Recent Developments in Sample Preparation for Mass Spectrometry-Based Metabolomics.

    PubMed

    Gong, Zhi-Gang; Hu, Jing; Wu, Xi; Xu, Yong-Jiang

    2017-07-04

    Metabolomics is a critical member in systems biology. Although great progress has been achieved in metabolomics, there are still some problems in sample preparation, data processing and data interpretation. In this review, we intend to explore the roles, challenges and trends in sample preparation for mass spectrometry- (MS-) based metabolomics. The newly emerged sample preparation methods were also critically examined, including laser microdissection, in vivo sampling, dried blood spot, microwave, ultrasound and enzyme-assisted extraction, as well as microextraction techniques. Finally, we provide some conclusions and perspectives for sample preparation in MS-based metabolomics.

  4. [Equipment qualification].

    PubMed

    Le Vacon, F

    2005-06-01

    The qualification of the equipment is a particularly important stage in the transfusional process. On the one hand, of many standards such as those of certification or that of accreditation require it, just as the good transfusional practices; in addition, the practices of steps of quality assurance develop this aspect. Indeed, the absence of the realization of this qualification of material having an influence on the finished product, can lead to an error in the product. This qualification passes by various stages of which some are major such as the drafting of the schedule of conditions, the drafting of the operational protocol of qualification, the decision made for the setting in routine. Finally so that this qualification takes all its dimensions it is necessary to carry out methods linked to the international system of measurement. Moreover certain questions after reflexions must find response such as which unit to check, and only this one, the equipment is - it a complex one, is there a maintenance contract? Once all these elements taken into account, the questions having found their answer, the operational protocol will then well be built, the decisions of settings in routine could be done and the sets of the finalized stages.

  5. Characterization of the tank 51 alternate reductant sludge batch 9 slurry sample (HTF-51-15-130)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Reboul, S. H.

    Tank 51 slurry sample HTF-51-15-130 was collected following sludge washing at the Tank Farm. The sample was received at SRNL and then characterized in preparation for qualification of the alternate reductant Sludge Batch 9 (SB9) flowsheet. In this characterization, densities, solids distribution, elemental constituents, anionic constituents, carbon content, and select radioisotopes were quantified.

  6. Australian Qualifications Framework Lower-Level Qualifications: Pathways to Where for Young People?

    ERIC Educational Resources Information Center

    Stanwick, John

    2005-01-01

    This study investigates where certificate I and II qualifications lead young people aged 15-24 years in terms of employment and further study. A prime motivation for young people undertaking these qualifications is to facilitate transition into the labour market. These qualifications are aimed at developing basic vocational skills or preparatory…

  7. 7 CFR 61.34 - Drawing and preparation of sample.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Drawing and preparation of sample. 61.34 Section 61.34 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... Cottonseed Samplers § 61.34 Drawing and preparation of sample. Each licensed cottonseed sampler shall draw...

  8. High-throughput automated microfluidic sample preparation for accurate microbial genomics

    PubMed Central

    Kim, Soohong; De Jonghe, Joachim; Kulesa, Anthony B.; Feldman, David; Vatanen, Tommi; Bhattacharyya, Roby P.; Berdy, Brittany; Gomez, James; Nolan, Jill; Epstein, Slava; Blainey, Paul C.

    2017-01-01

    Low-cost shotgun DNA sequencing is transforming the microbial sciences. Sequencing instruments are so effective that sample preparation is now the key limiting factor. Here, we introduce a microfluidic sample preparation platform that integrates the key steps in cells to sequence library sample preparation for up to 96 samples and reduces DNA input requirements 100-fold while maintaining or improving data quality. The general-purpose microarchitecture we demonstrate supports workflows with arbitrary numbers of reaction and clean-up or capture steps. By reducing the sample quantity requirements, we enabled low-input (∼10,000 cells) whole-genome shotgun (WGS) sequencing of Mycobacterium tuberculosis and soil micro-colonies with superior results. We also leveraged the enhanced throughput to sequence ∼400 clinical Pseudomonas aeruginosa libraries and demonstrate excellent single-nucleotide polymorphism detection performance that explained phenotypically observed antibiotic resistance. Fully-integrated lab-on-chip sample preparation overcomes technical barriers to enable broader deployment of genomics across many basic research and translational applications. PMID:28128213

  9. Proposed acceptance, qualification, and characterization tests for thin-film PV modules

    NASA Technical Reports Server (NTRS)

    Waddington, D.; Mrig, L.; Deblasio, R.; Ross, R.

    1988-01-01

    Details of a proposed test program for PV thin-film modules which the Department of Energy has directed the Solar Energy Research Institute (SERI) to prepare are presented. Results of one of the characterization tests that SERI has performed are also presented. The objective is to establish a common approach to testing modules that will be acceptable to both users and manufacturers. The tests include acceptance, qualification, and characterization tests. Acceptance tests verify that randomly selected modules have similar characteristics. Qualification tests are based on accelerated test methods designed to simulate adverse conditions. Characterization tests provide data on performance in a predefined environment.

  10. Effects of sample preparation on the infrared reflectance spectra of powders

    NASA Astrophysics Data System (ADS)

    Brauer, Carolyn S.; Johnson, Timothy J.; Myers, Tanya L.; Su, Yin-Fong; Blake, Thomas A.; Forland, Brenda M.

    2015-05-01

    While reflectance spectroscopy is a useful tool for identifying molecular compounds, laboratory measurement of solid (particularly powder) samples often is confounded by sample preparation methods. For example, both the packing density and surface roughness can have an effect on the quantitative reflectance spectra of powdered samples. Recent efforts in our group have focused on developing standard methods for measuring reflectance spectra that accounts for sample preparation, as well as other factors such as particle size and provenance. In this work, the effect of preparation method on sample reflectivity was investigated by measuring the directional-hemispherical spectra of samples that were hand-loaded as well as pressed into pellets using an integrating sphere attached to a Fourier transform infrared spectrometer. The results show that the methods used to prepare the sample can have a substantial effect on the measured reflectance spectra, as do other factors such as particle size.

  11. Fluidics platform and method for sample preparation and analysis

    DOEpatents

    Benner, W. Henry; Dzenitis, John M.; Bennet, William J.; Baker, Brian R.

    2014-08-19

    Herein provided are fluidics platform and method for sample preparation and analysis. The fluidics platform is capable of analyzing DNA from blood samples using amplification assays such as polymerase-chain-reaction assays and loop-mediated-isothermal-amplification assays. The fluidics platform can also be used for other types of assays and analyzes. In some embodiments, a sample in a sealed tube can be inserted directly. The following isolation, detection, and analyzes can be performed without a user's intervention. The disclosed platform may also comprises a sample preparation system with a magnetic actuator, a heater, and an air-drying mechanism, and fluid manipulation processes for extraction, washing, elution, assay assembly, assay detection, and cleaning after reactions and between samples.

  12. Effects of Sample Preparation on the Infrared Reflectance Spectra of Powders

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Brauer, Carolyn S.; Johnson, Timothy J.; Myers, Tanya L.

    2015-05-22

    While reflectance spectroscopy is a useful tool in identifying molecular compounds, laboratory measurement of solid (particularly powder) samples often is confounded by sample preparation methods. For example, both the packing density and surface roughness can have an effect on the quantitative reflectance spectra of powdered samples. Recent efforts in our group have focused on developing standard methods for measuring reflectance spectra that accounts for sample preparation, as well as other factors such as particle size and provenance. In this work, the effect of preparation method on sample reflectivity was investigated by measuring the directional-hemispherical spectra of samples that were hand-packedmore » as well as pressed into pellets using an integrating sphere attached to a Fourier transform infrared spectrometer. The results show that the methods used to prepare the sample have a substantial effect on the measured reflectance spectra, as do other factors such as particle size.« less

  13. VERIFICATION OF THE DEFENSE WASTE PROCESSING FACILITY PROCESS DIGESTION METHOD FOR THE SLUDGE BATCH 6 QUALIFICATION SAMPLE

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Click, D.; Jones, M.; Edwards, T.

    2010-06-09

    For each sludge batch that is processed in the Defense Waste Processing Facility (DWPF), the Savannah River National Laboratory (SRNL) confirms applicability of the digestion method to be used by the DWPF lab for elemental analysis of Sludge Receipt and Adjustment Tank (SRAT) receipt samples and SRAT product process control samples.1 DWPF SRAT samples are typically dissolved using a room temperature HF-HNO3 acid dissolution (i.e., DWPF Cold Chem (CC) Method, see DWPF Procedure SW4-15.201) and then analyzed by inductively coupled plasma - atomic emission spectroscopy (ICPAES). In addition to the CC method confirmation, the DWPF lab's mercury (Hg) digestion methodmore » was also evaluated for applicability to SB6 (see DWPF procedure 'Mercury System Operating Manual', Manual: SW4-15.204. Section 6.1, Revision 5, Effective date: 12-04-03). This report contains the results and comparison of data generated from performing the Aqua Regia (AR), Sodium Peroxide/Hydroxide Fusion (PF) and DWPF Cold Chem (CC) method digestion of Sludge Batch 6 (SB6) SRAT Receipt and SB6 SRAT Product samples. For validation of the DWPF lab's Hg method, only SRAT receipt material was used and compared to AR digestion results. The SB6 SRAT Receipt and SB6 SRAT Product samples were prepared in the SRNL Shielded Cells, and the SRAT Receipt material is representative of the sludge that constitutes the SB6 Batch or qualification composition. This is the sludge in Tank 51 that is to be transferred into Tank 40, which will contain the heel of Sludge Batch 5 (SB5), to form the SB6 Blend composition. In addition to the 16 elements currently measured by the DWPF, this report includes Hg and thorium (Th) data (Th comprising {approx}2.5 - 3 Wt% of the total solids in SRAT Receipt and SRAT Product, respectively) and provides specific details of ICP-AES analysis of Th. Thorium was found to interfere with the U 367.007 nm emission line, and an inter-element correction (IEC) had to be applied to U data, which

  14. New Methods of Sample Preparation for Atom Probe Specimens

    NASA Technical Reports Server (NTRS)

    Kuhlman, Kimberly, R.; Kowalczyk, Robert S.; Ward, Jennifer R.; Wishard, James L.; Martens, Richard L.; Kelly, Thomas F.

    2003-01-01

    Magnetite is a common conductive mineral found on Earth and Mars. Disk-shaped precipitates approximately 40 nm in diameter have been shown to have manganese and aluminum concentrations. Atom-probe field-ion microscopy (APFIM) is the only technique that can potentially quantify the composition of these precipitates. APFIM will be used to characterize geological and planetary materials, analyze samples of interest for geomicrobiology; and, for the metrology of nanoscale instrumentation. Prior to APFIM sample preparation was conducted by electropolishing, the method of sharp shards (MSS), or Bosch process (deep reactive ion etching) with focused ion beam (FIB) milling as a final step. However, new methods are required for difficult samples. Many materials are not easily fabricated using electropolishing, MSS, or the Bosch process, FIB milling is slow and expensive, and wet chemistry and the reactive ion etching are typically limited to Si and other semiconductors. APFIM sample preparation using the dicing saw is commonly used to section semiconductor wafers into individual devices following manufacture. The dicing saw is a time-effective method for preparing high aspect ratio posts of poorly conducting materials. Femtosecond laser micromachining is also suitable for preparation of posts. FIB time required is reduced by about a factor of 10 and multi-tip specimens can easily be fabricated using the dicing saw.

  15. Sample Preparation for Mass Spectrometry Imaging of Plant Tissues: A Review

    PubMed Central

    Dong, Yonghui; Li, Bin; Malitsky, Sergey; Rogachev, Ilana; Aharoni, Asaph; Kaftan, Filip; Svatoš, Aleš; Franceschi, Pietro

    2016-01-01

    Mass spectrometry imaging (MSI) is a mass spectrometry based molecular ion imaging technique. It provides the means for ascertaining the spatial distribution of a large variety of analytes directly on tissue sample surfaces without any labeling or staining agents. These advantages make it an attractive molecular histology tool in medical, pharmaceutical, and biological research. Likewise, MSI has started gaining popularity in plant sciences; yet, information regarding sample preparation methods for plant tissues is still limited. Sample preparation is a crucial step that is directly associated with the quality and authenticity of the imaging results, it therefore demands in-depth studies based on the characteristics of plant samples. In this review, a sample preparation pipeline is discussed in detail and illustrated through selected practical examples. In particular, special concerns regarding sample preparation for plant imaging are critically evaluated. Finally, the applications of MSI techniques in plants are reviewed according to different classes of plant metabolites. PMID:26904042

  16. Preparation of Chemical Samples On Relevant Surfaces Using Inkjet Technology

    DTIC Science & Technology

    2013-04-01

    PREPARATION OF CHEMICAL SAMPLES ON RELEVANT SURFACES USING INKJET TECHNOLOGY...2012 4. TITLE AND SUBTITLE 5a. CONTRACT NUMBER Preparation of Chemical Samples on Relevant Surfaces Using Inkjet Technology 5b. GRANT NUMBER...SUBJECT TERMS Surface detection Inkjet Simulant deposition 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT 18. NUMBER OF

  17. 42 CFR 61.34 - Qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Qualifications. 61.34 Section 61.34 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Service Fellowships § 61.34 Qualifications. Scholastic and other qualifications shall be...

  18. 42 CFR 61.34 - Qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Qualifications. 61.34 Section 61.34 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Service Fellowships § 61.34 Qualifications. Scholastic and other qualifications shall be...

  19. 42 CFR 61.34 - Qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false Qualifications. 61.34 Section 61.34 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Service Fellowships § 61.34 Qualifications. Scholastic and other qualifications shall be...

  20. 42 CFR 61.34 - Qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false Qualifications. 61.34 Section 61.34 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Service Fellowships § 61.34 Qualifications. Scholastic and other qualifications shall be...

  1. 42 CFR 61.34 - Qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 1 2013-10-01 2013-10-01 false Qualifications. 61.34 Section 61.34 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Service Fellowships § 61.34 Qualifications. Scholastic and other qualifications shall be...

  2. Lightning arrestor connector lead magnesium niobate qualification pellet test procedures.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tuohig, W.; Mahoney, Patrick A.; Tuttle, Bruce Andrew

    2009-02-01

    Enhanced knowledge preservation for DOE DP technical component activities has recently received much attention. As part of this recent knowledge preservation effort, improved documentation of the sample preparation and electrical testing procedures for lead magnesium niobate--lead titanate (PMN/PT) qualification pellets was completed. The qualification pellets are fabricated from the same parent powders used to produce PMN/PT lightning arrestor connector (LAC) granules at HWF&T. In our report, the procedures for fired pellet surface preparation, electrode deposition, electrical testing and data recording are described. The dielectric measurements described in our report are an information only test. Technical reasons for selecting the electrodemore » material, electrode size and geometry are presented. The electrical testing is based on measuring the dielectric constant and dissipation factor of the pellet during cooling from 280 C to 220 C. The most important data are the temperature for which the peak dielectric constant occurs (Curie Point temperature) and the peak dielectric constant magnitude. We determined that the peak dielectric constant for our procedure would be that measured at 1 kHz at the Curie Point. Both the peak dielectric constant and the Curie point parameters provide semi-quantitative information concerning the chemical and microstructural homogeneity of the parent material used for the production of PMN/PT granules for LACs. Finally, we have proposed flag limits for the dielectric data for the pellets. Specifically, if the temperature of the peak dielectric constant falls outside the range of 250 C {+-} 30 C we propose that a flag limit be imposed that will initiate communication between production agency and design agency personnel. If the peak dielectric constant measured falls outside the range 25,000 {+-} 10,000 we also propose that a flag limit be imposed.« less

  3. Sample preparation and EFTEM of Meat Samples for Nanoparticle Analysis in Food

    NASA Astrophysics Data System (ADS)

    Lari, L.; Dudkiewicz, A.

    2014-06-01

    Nanoparticles are used in industry for personal care products and the preparation of food. In the latter application, their functions include the prevention of microbes' growth, increase of the foods nutritional value and sensory quality. EU regulations require a risk assessment of the nanoparticles used in foods and food contact materials before the products can reach the market. However, availability of validated analytical methodologies for detection and characterisation of the nanoparticles in food hampers appropriate risk assessment. As part of a research on the evaluation of the methods for screening and quantification of Ag nanoparticles in meat we have tested a new TEM sample preparation alternative to resin embedding and cryo-sectioning. Energy filtered TEM analysis was applied to evaluate thickness and the uniformity of thin meat layers acquired at increasing input of the sample demonstrating that the protocols used ensured good stability under the electron beam, reliable sample concentration and reproducibility.

  4. Final LDRD report : development of sample preparation methods for ChIPMA-based imaging mass spectrometry of tissue samples.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Maharrey, Sean P.; Highley, Aaron M.; Behrens, Richard, Jr.

    2007-12-01

    The objective of this short-term LDRD project was to acquire the tools needed to use our chemical imaging precision mass analyzer (ChIPMA) instrument to analyze tissue samples. This effort was an outgrowth of discussions with oncologists on the need to find the cellular origin of signals in mass spectra of serum samples, which provide biomarkers for ovarian cancer. The ultimate goal would be to collect chemical images of biopsy samples allowing the chemical images of diseased and nondiseased sections of a sample to be compared. The equipment needed to prepare tissue samples have been acquired and built. This equipment includesmore » an cyro-ultramicrotome for preparing thin sections of samples and a coating unit. The coating unit uses an electrospray system to deposit small droplets of a UV-photo absorbing compound on the surface of the tissue samples. Both units are operational. The tissue sample must be coated with the organic compound to enable matrix assisted laser desorption/ionization (MALDI) and matrix enhanced secondary ion mass spectrometry (ME-SIMS) measurements with the ChIPMA instrument Initial plans to test the sample preparation using human tissue samples required development of administrative procedures beyond the scope of this LDRD. Hence, it was decided to make two types of measurements: (1) Testing the spatial resolution of ME-SIMS by preparing a substrate coated with a mixture of an organic matrix and a bio standard and etching a defined pattern in the coating using a liquid metal ion beam, and (2) preparing and imaging C. elegans worms. Difficulties arose in sectioning the C. elegans for analysis and funds and time to overcome these difficulties were not available in this project. The facilities are now available for preparing biological samples for analysis with the ChIPMA instrument. Some further investment of time and resources in sample preparation should make this a useful tool for chemical imaging applications.« less

  5. Optimization for Peptide Sample Preparation for Urine Peptidomics

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sigdel, Tara K.; Nicora, Carrie D.; Hsieh, Szu-Chuan

    2014-02-25

    Analysis of native or endogenous peptides in biofluids can provide valuable insights into disease mechanisms. Furthermore, the detected peptides may also have utility as potential biomarkers for non-invasive monitoring of human diseases. The non-invasive nature of urine collection and the abundance of peptides in the urine makes analysis by high-throughput ‘peptidomics’ methods , an attractive approach for investigating the pathogenesis of renal disease. However, urine peptidomics methodologies can be problematic with regards to difficulties associated with sample preparation. The urine matrix can provide significant background interference in making the analytical measurements that it hampers both the identification of peptides andmore » the depth of the peptidomics read when utilizing LC-MS based peptidome analysis. We report on a novel adaptation of the standard solid phase extraction (SPE) method to a modified SPE (mSPE) approach for improved peptide yield and analysis sensitivity with LC-MS based peptidomics in terms of time, cost, clogging of the LC-MS column, peptide yield, peptide quality, and number of peptides identified by each method. Expense and time requirements were comparable for both SPE and mSPE, but more interfering contaminants from the urine matrix were evident in the SPE preparations (e.g., clogging of the LC-MS columns, yellowish background coloration of prepared samples due to retained urobilin, lower peptide yields) when compared to the mSPE method. When we compared data from technical replicates of 4 runs, the mSPE method provided significantly improved efficiencies for the preparation of samples from urine (e.g., mSPE peptide identification 82% versus 18% with SPE; p = 8.92E-05). Additionally, peptide identifications, when applying the mSPE method, highlighted the biology of differential activation of urine peptidases during acute renal transplant rejection with distinct laddering of specific peptides, which was obscured for most

  6. Preparation of protein samples for mass spectrometry and N-terminal sequencing.

    PubMed

    Glenn, Gary

    2014-01-01

    The preparation of protein samples for mass spectrometry and N-terminal sequencing is a key step in successfully identifying proteins. Mass spectrometry is a very sensitive technique, and as such, samples must be prepared carefully since they can be subject to contamination of the sample (e.g., due to incomplete subcellular fractionation or purification of a multiprotein complex), overwhelming of the sample by highly abundant proteins, and contamination from skin or hair (keratin can be a very common hit). One goal of sample preparation for mass spec is to reduce the complexity of the sample - in the example presented here, mitochondria are purified, solubilized, and fractionated by sucrose density gradient sedimentation prior to preparative 1D SDS-PAGE. It is important to verify the purity and integrity of the sample so that you can have confidence in the hits obtained. More protein is needed for N-terminal sequencing and ideally it should be purified to a single band when run on an SDS-polyacrylamide gel. The example presented here involves stably expressing a tagged protein in HEK293 cells and then isolating the protein by affinity purification and SDS-PAGE. © 2014 Elsevier Inc. All rights reserved.

  7. Analysis of tank 4 (FTF-4-15-22, 23) surface and subsurface supernatant samples in support of enrichment control, corrosion control and evaporator feed qualification programs

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Oji, L. N.

    This report provides the results of analyses on Savannah River Site Tank 4 surface and subsurface supernatant liquid samples in support of the Enrichment Control Program (ECP), the Corrosion Control Program (CCP) and the Evaporator Feed Qualification (EFQ) Program. The purpose of the ECP sample taken from Tank 4 in August 2015 was to determine if the supernatant liquid would be “acceptable feed” to the 2H and 3H evaporator systems.

  8. GUM Analysis for TIMS Isotopic Ratios in BEP0 Graphite Qualification Samples, Round 2

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gerlach, David C.; Heasler, Patrick G.; Reid, Bruce D.

    In May 2007, one set of three samples from NBL were addressed to Steve Petersen for TIMS analysis, and included BEP0 samples numbered 27008, 30986, and 50846. All cores were trimmed by tooling, and lightly cleaned by CO2 pellet blasting. Small discs were cut from the second set of samples for SIMS analysis, with the remainder of each used for TIMS preparation.

  9. Novel strategies for sample preparation in forensic toxicology.

    PubMed

    Samanidou, Victoria; Kovatsi, Leda; Fragou, Domniki; Rentifis, Konstantinos

    2011-09-01

    This paper provides a review of novel strategies for sample preparation in forensic toxicology. The review initially outlines the principle of each technique, followed by sections addressing each class of abused drugs separately. The novel strategies currently reviewed focus on the preparation of various biological samples for the subsequent determination of opiates, benzodiazepines, amphetamines, cocaine, hallucinogens, tricyclic antidepressants, antipsychotics and cannabinoids. According to our experience, these analytes are the most frequently responsible for intoxications in Greece. The applications of techniques such as disposable pipette extraction, microextraction by packed sorbent, matrix solid-phase dispersion, solid-phase microextraction, polymer monolith microextraction, stir bar sorptive extraction and others, which are rapidly gaining acceptance in the field of toxicology, are currently reviewed.

  10. 5 CFR 302.403 - Qualifications for promotion.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Qualifications for promotion. 302.403... EMPLOYMENT IN THE EXCEPTED SERVICE Selection and Appointment; Reappointment; and Qualifications for Promotion § 302.403 Qualifications for promotion. In determining qualifications for promotion with respect to an...

  11. 34 CFR 300.156 - Personnel qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... children with disabilities. (c) Qualifications for special education teachers. The qualifications described... personnel to provide special education and related services under this part to children with disabilities... 34 Education 2 2011-07-01 2010-07-01 true Personnel qualifications. 300.156 Section 300.156...

  12. 5 CFR 319.302 - Individual qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Individual qualifications. 319.302... Individual qualifications. Agency heads are delegated authority to approve the qualifications of individuals appointed to SL and ST positions. The agency head must determine that the individual meets the...

  13. 10 CFR 603.510 - Recipient qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Recipient qualifications. 603.510 Section 603.510 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS TECHNOLOGY INVESTMENT AGREEMENTS Pre-Award Business Evaluation Recipient Qualification § 603.510 Recipient qualifications. Prior to award of a TIA, the...

  14. 10 CFR 603.510 - Recipient qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Recipient qualifications. 603.510 Section 603.510 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS TECHNOLOGY INVESTMENT AGREEMENTS Pre-Award Business Evaluation Recipient Qualification § 603.510 Recipient qualifications. Prior to award of a TIA, the...

  15. Final Report for X-ray Diffraction Sample Preparation Method Development

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ely, T. M.; Meznarich, H. K.; Valero, T.

    WRPS-1500790, “X-ray Diffraction Saltcake Sample Preparation Method Development Plan/Procedure,” was originally prepared with the intent of improving the specimen preparation methodology used to generate saltcake specimens suitable for XRD-based solid phase characterization. At the time that this test plan document was originally developed, packed powder in cavity supports with collodion binder was the established XRD specimen preparation method. An alternate specimen preparation method less vulnerable, if not completely invulnerable to preferred orientation effects, was desired as a replacement for the method.

  16. Extraction, -scrub, -strip test results from the interim salt disposition program macrobatch 10 tank 21H qualification samples

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Peters, T. B.

    Savannah River National Laboratory (SRNL) analyzed samples from Tank 21H in support of qualification of Macrobatch (Salt Batch) 10 for the Interim Salt Disposition Program (ISDP). The Salt Batch 10 characterization results were previously reported.ii,iii An Extraction, -Scrub, -Strip (ESS) test was performed to determine cesium distribution ratios (D(Cs)) and cesium concentration in the strip effluent (SE) and decontaminated salt solution (DSS) streams; this data will be used by Tank Farm Engineering to project a cesium decontamination factor (DF). This test used actual Tank 21H material, and a sample of the NGS Blend solvent currently being used at the Modularmore » Caustic-Side Solvent Extraction Unit (MCU). The ESS test showed acceptable performance with an extraction D(Cs) value of 110. This value is consistent with results from previous salt batch ESS tests using similar solvent formulations. This is better than the predicted value of 39.8 from a recently created D(Cs) model.« less

  17. Liquid Secondary Waste Grout Formulation and Waste Form Qualification

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Um, Wooyong; Williams, B. D.; Snyder, Michelle M. V.

    This report describes the results from liquid secondary waste (LSW) grout formulation and waste form qualification tests performed at Pacific Northwest National Laboratory (PNNL) for Washington River Protection Solutions (WRPS) to evaluate new formulations for preparing a grout waste form with high-sulfate secondary waste simulants and the release of key constituents from these grout monoliths. Specific objectives of the LSW grout formulation and waste form qualification tests described in this report focused on five activities: 1.preparing new formulations for the LSW grout waste form with high-sulfate LSW simulants and solid characterization of the cured LSW grout waste form; 2.conducting themore » U.S. Environmental Protection Agency (EPA) Method 1313 leach test (EPA 2012) on the grout prepared with the new formulations, which solidify sulfate-rich Hanford Tank Waste Treatment and Immobilization Plant (WTP) off-gas condensate secondary waste simulant, using deionized water (DIW); 3.conducting the EPA Method 1315 leach tests (EPA 2013) on the grout monoliths made with the new dry blend formulations and three LSW simulants (242-A evaporator condensate, Environmental Restoration Disposal Facility (ERDF) leachate, and WTP off-gas condensate) using two leachants, DIW and simulated Hanford Integrated Disposal Facility (IDF) Site vadose zone pore water (VZPW); 4.estimating the 99Tc desorption K d (distribution coefficient) values for 99Tc transport in oxidizing conditions to support the IDF performance assessment (PA); 5.estimating the solubility of 99Tc(IV)-bearing solid phases for 99Tc transport in reducing conditions to support the IDF PA.« less

  18. Automated SEM and TEM sample preparation applied to copper/low k materials

    NASA Astrophysics Data System (ADS)

    Reyes, R.; Shaapur, F.; Griffiths, D.; Diebold, A. C.; Foran, B.; Raz, E.

    2001-01-01

    We describe the use of automated microcleaving for preparation of both SEM and TEM samples as done by SELA's new MC500 and TEMstation tools. The MC500 is an automated microcleaving tool that is capable of producing cleaves with 0.25 μm accuracy resulting in SEM-ready samples. The TEMstation is capable of taking a sample output from the MC500 (or from SELA's earlier MC200 tool) and producing a FIB ready slice of 25±5 μm, mounted on a TEM-washer and ready for FIB thinning to electron transparency for TEM analysis. The materials selected for the tool set evaluation mainly included the Cu/TaN/HOSP low-k system. The paper is divided into three sections, experimental approach, SEM preparation and analysis of HOSP low-k, and TEM preparation and analysis of Cu/TaN/HOSP low-k samples. For the samples discussed, data is presented to show the quality of preparation provided by these new automated tools.

  19. 29 CFR 32.14 - Job qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 1 2014-07-01 2013-07-01 true Job qualifications. 32.14 Section 32.14 Labor Office of the... FINANCIAL ASSISTANCE Employment Practices and Employment Related Training Participation § 32.14 Job... appropriateness of all job qualifications to ensure that to the extent job qualifications tend to exclude...

  20. 29 CFR 32.14 - Job qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 1 2013-07-01 2013-07-01 false Job qualifications. 32.14 Section 32.14 Labor Office of the... FINANCIAL ASSISTANCE Employment Practices and Employment Related Training Participation § 32.14 Job... appropriateness of all job qualifications to ensure that to the extent job qualifications tend to exclude...

  1. 29 CFR 32.14 - Job qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Job qualifications. 32.14 Section 32.14 Labor Office of the... FINANCIAL ASSISTANCE Employment Practices and Employment Related Training Participation § 32.14 Job... appropriateness of all job qualifications to ensure that to the extent job qualifications tend to exclude...

  2. 29 CFR 32.14 - Job qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 1 2012-07-01 2012-07-01 false Job qualifications. 32.14 Section 32.14 Labor Office of the... FINANCIAL ASSISTANCE Employment Practices and Employment Related Training Participation § 32.14 Job... appropriateness of all job qualifications to ensure that to the extent job qualifications tend to exclude...

  3. 29 CFR 32.14 - Job qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 1 2011-07-01 2011-07-01 false Job qualifications. 32.14 Section 32.14 Labor Office of the... FINANCIAL ASSISTANCE Employment Practices and Employment Related Training Participation § 32.14 Job... appropriateness of all job qualifications to ensure that to the extent job qualifications tend to exclude...

  4. 10 CFR 603.510 - Recipient qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Evaluation Recipient Qualification § 603.510 Recipient qualifications. Prior to award of a TIA, the... for awarding a grant or cooperative agreement. If the recipient is a consortium that is not formally... 10 Energy 4 2014-01-01 2014-01-01 false Recipient qualifications. 603.510 Section 603.510 Energy...

  5. 10 CFR 603.510 - Recipient qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Evaluation Recipient Qualification § 603.510 Recipient qualifications. Prior to award of a TIA, the... for awarding a grant or cooperative agreement. If the recipient is a consortium that is not formally... 10 Energy 4 2011-01-01 2011-01-01 false Recipient qualifications. 603.510 Section 603.510 Energy...

  6. 10 CFR 603.510 - Recipient qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Evaluation Recipient Qualification § 603.510 Recipient qualifications. Prior to award of a TIA, the... for awarding a grant or cooperative agreement. If the recipient is a consortium that is not formally... 10 Energy 4 2012-01-01 2012-01-01 false Recipient qualifications. 603.510 Section 603.510 Energy...

  7. Polymeric ionic liquid-based portable tip microextraction device for on-site sample preparation of water samples.

    PubMed

    Chen, Lei; Pei, Junxian; Huang, Xiaojia; Lu, Min

    2018-06-05

    On-site sample preparation is highly desired because it avoids the transportation of large-volume samples and ensures the accuracy of the analytical results. In this work, a portable prototype of tip microextraction device (TMD) was designed and developed for on-site sample pretreatment. The assembly procedure of TMD is quite simple. Firstly, polymeric ionic liquid (PIL)-based adsorbent was in-situ prepared in a pipette tip. After that, the tip was connected with a syringe which was driven by a bidirectional motor. The flow rates in adsorption and desorption steps were controlled accurately by the motor. To evaluate the practicability of the developed device, the TMD was used to on-site sample preparation of waters and combined with high-performance liquid chromatography with diode array detection to measure trace estrogens in water samples. Under the most favorable conditions, the limits of detection (LODs, S/N = 3) for the target analytes were in the range of 4.9-22 ng/L, with good coefficients of determination. Confirmatory study well evidences that the extraction performance of TMD is comparable to that of the traditional laboratory solid-phase extraction process, but the proposed TMD is more simple and convenient. At the same time, the TMD avoids complicated sampling and transferring steps of large-volume water samples. Copyright © 2018 Elsevier B.V. All rights reserved.

  8. Optimization of Sample Preparation processes of Bone Material for Raman Spectroscopy.

    PubMed

    Chikhani, Madelen; Wuhrer, Richard; Green, Hayley

    2018-03-30

    Raman spectroscopy has recently been investigated for use in the calculation of postmortem interval from skeletal material. The fluorescence generated by samples, which affects the interpretation of Raman data, is a major limitation. This study compares the effectiveness of two sample preparation techniques, chemical bleaching and scraping, in the reduction of fluorescence from bone samples during testing with Raman spectroscopy. Visual assessment of Raman spectra obtained at 1064 nm excitation following the preparation protocols indicates an overall reduction in fluorescence. Results demonstrate that scraping is more effective at resolving fluorescence than chemical bleaching. The scraping of skeletonized remains prior to Raman analysis is a less destructive method and allows for the preservation of a bone sample in a state closest to its original form, which is beneficial in forensic investigations. It is recommended that bone scraping supersedes chemical bleaching as the preferred method for sample preparation prior to Raman spectroscopy. © 2018 American Academy of Forensic Sciences.

  9. Magnetic separation techniques in sample preparation for biological analysis: a review.

    PubMed

    He, Jincan; Huang, Meiying; Wang, Dongmei; Zhang, Zhuomin; Li, Gongke

    2014-12-01

    Sample preparation is a fundamental and essential step in almost all the analytical procedures, especially for the analysis of complex samples like biological and environmental samples. In past decades, with advantages of superparamagnetic property, good biocompatibility and high binding capacity, functionalized magnetic materials have been widely applied in various processes of sample preparation for biological analysis. In this paper, the recent advancements of magnetic separation techniques based on magnetic materials in the field of sample preparation for biological analysis were reviewed. The strategy of magnetic separation techniques was summarized. The synthesis, stabilization and bio-functionalization of magnetic nanoparticles were reviewed in detail. Characterization of magnetic materials was also summarized. Moreover, the applications of magnetic separation techniques for the enrichment of protein, nucleic acid, cell, bioactive compound and immobilization of enzyme were described. Finally, the existed problems and possible trends of magnetic separation techniques for biological analysis in the future were proposed. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. Sample Preparation Techniques for Grain Boundary Characterization of Annealed TRISO-Coated Particles

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dunzik-Gougar, M. L.; van Rooyen, I. J.; Hill, C. M.

    Crystallographic information about chemical vapor deposition layers of silicon carbide (SiC) is essential to understanding layer performance, especially when the layers are in non planar geometries, such as spherical. We performed electron Back Scatter Diffraction (EBSD) analysis of spherical SiC layers using a different approach to sample focus ion beam milling technique to avoid the negative impacts of traditional sample polishing and to address the need of very small samples of irradiated materials for analysis. Mechanical and chemical grinding and polishing of sample surfaces can introduce lattice strains and result in unequal removal of SiC and surrounding layers of differentmore » material due to the hardness differences of these materials. The nature of layer interfaces is thought to play a key role in performance of the SiC; therefore, analysis of representative samples at these interfacial areas is crucial. In work reported here, a focused ion beam (FIB) was employed in a novel manner to prepare a more representative sample for EBSD analysis from TRISO layers free of effects introduced by mechanical and chemical preparation methods. In addition, the difficulty of handling neutron irradiated microscopic samples such as those analyzed in this work has been simplified with pre tilted mounting stages. Our study showed that although the average grain size of samples may be similar, the grain boundary characteristics may differ significantly. It was also found that low angle grain boundaries, comprises 25% in the FIB-prepared sample vs only 1-2% in the polished sample measured in the same particle. From this study it was determined that results of FIB prepared sample will provide more repeatable results, as the role of sample preparation is eliminated.« less

  11. Sample Preparation Techniques for Grain Boundary Characterization of Annealed TRISO-Coated Particles

    DOE PAGES

    Dunzik-Gougar, M. L.; van Rooyen, I. J.; Hill, C. M.; ...

    2016-08-25

    Crystallographic information about chemical vapor deposition layers of silicon carbide (SiC) is essential to understanding layer performance, especially when the layers are in non planar geometries, such as spherical. We performed electron Back Scatter Diffraction (EBSD) analysis of spherical SiC layers using a different approach to sample focus ion beam milling technique to avoid the negative impacts of traditional sample polishing and to address the need of very small samples of irradiated materials for analysis. Mechanical and chemical grinding and polishing of sample surfaces can introduce lattice strains and result in unequal removal of SiC and surrounding layers of differentmore » material due to the hardness differences of these materials. The nature of layer interfaces is thought to play a key role in performance of the SiC; therefore, analysis of representative samples at these interfacial areas is crucial. In work reported here, a focused ion beam (FIB) was employed in a novel manner to prepare a more representative sample for EBSD analysis from TRISO layers free of effects introduced by mechanical and chemical preparation methods. In addition, the difficulty of handling neutron irradiated microscopic samples such as those analyzed in this work has been simplified with pre tilted mounting stages. Our study showed that although the average grain size of samples may be similar, the grain boundary characteristics may differ significantly. It was also found that low angle grain boundaries, comprises 25% in the FIB-prepared sample vs only 1-2% in the polished sample measured in the same particle. From this study it was determined that results of FIB prepared sample will provide more repeatable results, as the role of sample preparation is eliminated.« less

  12. Preparation of Cytology Samples: Tricks of the Trade.

    PubMed

    Moore, A Russell

    2017-01-01

    General principles and techniques for collection, preparation, and staining of cytologic samples in the general practice setting are reviewed. Tips for collection of digital images are also discussed. Copyright © 2016 Elsevier Inc. All rights reserved.

  13. Assessment of Sample Preparation Bias in Mass Spectrometry-Based Proteomics.

    PubMed

    Klont, Frank; Bras, Linda; Wolters, Justina C; Ongay, Sara; Bischoff, Rainer; Halmos, Gyorgy B; Horvatovich, Péter

    2018-04-17

    For mass spectrometry-based proteomics, the selected sample preparation strategy is a key determinant for information that will be obtained. However, the corresponding selection is often not based on a fit-for-purpose evaluation. Here we report a comparison of in-gel (IGD), in-solution (ISD), on-filter (OFD), and on-pellet digestion (OPD) workflows on the basis of targeted (QconCAT-multiple reaction monitoring (MRM) method for mitochondrial proteins) and discovery proteomics (data-dependent acquisition, DDA) analyses using three different human head and neck tissues (i.e., nasal polyps, parotid gland, and palatine tonsils). Our study reveals differences between the sample preparation methods, for example, with respect to protein and peptide losses, quantification variability, protocol-induced methionine oxidation, and asparagine/glutamine deamidation as well as identification of cysteine-containing peptides. However, none of the methods performed best for all types of tissues, which argues against the existence of a universal sample preparation method for proteome analysis.

  14. 14 CFR 60.27 - Automatic loss of qualification and procedures for restoration of qualification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Automatic loss of qualification and procedures for restoration of qualification. 60.27 Section 60.27 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

  15. 14 CFR 60.29 - Other losses of qualification and procedures for restoration of qualification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Other losses of qualification and procedures for restoration of qualification. 60.29 Section 60.29 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

  16. 14 CFR 60.27 - Automatic loss of qualification and procedures for restoration of qualification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Automatic loss of qualification and procedures for restoration of qualification. 60.27 Section 60.27 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

  17. 14 CFR 60.29 - Other losses of qualification and procedures for restoration of qualification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Other losses of qualification and procedures for restoration of qualification. 60.29 Section 60.29 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

  18. 14 CFR 60.29 - Other losses of qualification and procedures for restoration of qualification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Other losses of qualification and procedures for restoration of qualification. 60.29 Section 60.29 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

  19. 14 CFR 60.29 - Other losses of qualification and procedures for restoration of qualification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Other losses of qualification and procedures for restoration of qualification. 60.29 Section 60.29 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

  20. 14 CFR 60.27 - Automatic loss of qualification and procedures for restoration of qualification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Automatic loss of qualification and procedures for restoration of qualification. 60.27 Section 60.27 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

  1. 14 CFR 60.29 - Other losses of qualification and procedures for restoration of qualification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Other losses of qualification and procedures for restoration of qualification. 60.29 Section 60.29 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

  2. 14 CFR 60.27 - Automatic loss of qualification and procedures for restoration of qualification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Automatic loss of qualification and procedures for restoration of qualification. 60.27 Section 60.27 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

  3. 14 CFR 60.27 - Automatic loss of qualification and procedures for restoration of qualification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Automatic loss of qualification and procedures for restoration of qualification. 60.27 Section 60.27 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND...

  4. 49 CFR 391.41 - Physical qualifications for drivers.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 5 2012-10-01 2012-10-01 false Physical qualifications for drivers. 391.41... QUALIFICATIONS OF DRIVERS AND LONGER COMBINATION VEHICLE (LCV) DRIVER INSTRUCTORS Physical Qualifications and Examinations § 391.41 Physical qualifications for drivers. (a)(1)(i) A person subject to this part must not...

  5. 49 CFR 391.41 - Physical qualifications for drivers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 5 2011-10-01 2011-10-01 false Physical qualifications for drivers. 391.41... QUALIFICATIONS OF DRIVERS AND LONGER COMBINATION VEHICLE (LCV) DRIVER INSTRUCTORS Physical Qualifications and Examinations § 391.41 Physical qualifications for drivers. (a)(1)(i) A person subject to this part must not...

  6. 49 CFR 391.41 - Physical qualifications for drivers.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 5 2013-10-01 2013-10-01 false Physical qualifications for drivers. 391.41... QUALIFICATIONS OF DRIVERS AND LONGER COMBINATION VEHICLE (LCV) DRIVER INSTRUCTORS Physical Qualifications and Examinations § 391.41 Physical qualifications for drivers. (a)(1)(i) A person subject to this part must not...

  7. 49 CFR 391.41 - Physical qualifications for drivers.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 5 2014-10-01 2014-10-01 false Physical qualifications for drivers. 391.41... QUALIFICATIONS OF DRIVERS AND LONGER COMBINATION VEHICLE (LCV) DRIVER INSTRUCTORS Physical Qualifications and Examinations § 391.41 Physical qualifications for drivers. (a)(1)(i) A person subject to this part must not...

  8. 14 CFR 121.915 - Continuing qualification curriculum.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Continuing qualification curriculum. 121... Continuing qualification curriculum. Each continuing qualification curriculum must contain training and... training device as approved in the certificate holder's curriculum that must be completed during each...

  9. Applications of reversible covalent chemistry in analytical sample preparation.

    PubMed

    Siegel, David

    2012-12-07

    Reversible covalent chemistry (RCC) adds another dimension to commonly used sample preparation techniques like solid-phase extraction (SPE), solid-phase microextraction (SPME), molecular imprinted polymers (MIPs) or immuno-affinity cleanup (IAC): chemical selectivity. By selecting analytes according to their covalent reactivity, sample complexity can be reduced significantly, resulting in enhanced analytical performance for low-abundance target analytes. This review gives a comprehensive overview of the applications of RCC in analytical sample preparation. The major reactions covered include reversible boronic ester formation, thiol-disulfide exchange and reversible hydrazone formation, targeting analyte groups like diols (sugars, glycoproteins and glycopeptides, catechols), thiols (cysteinyl-proteins and cysteinyl-peptides) and carbonyls (carbonylated proteins, mycotoxins). Their applications range from low abundance proteomics to reversible protein/peptide labelling to antibody chromatography to quantitative and qualitative food analysis. In discussing the potential of RCC, a special focus is on the conditions and restrictions of the utilized reaction chemistry.

  10. 49 CFR 180.507 - Qualification of tank cars.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Qualification of tank cars. 180.507 Section 180... QUALIFICATION AND MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.507 Qualification of tank cars. (a) Each tank car marked as meeting a “DOT” specification or any other tank car used...

  11. Social Partnership in Accrediting Lithuanian VET Qualifications

    ERIC Educational Resources Information Center

    Tutlys, Vidmantas; Kaminskiene, Lina

    2008-01-01

    This article examines social partnership in accrediting qualifications in Lithuania. It defines the factors influencing social partnership and surveys future development perspectives, referring to the creation and implementation of the national qualifications system in Lithuania. Social partnership in qualifications accreditation is regarded as a…

  12. MARS: bringing the automation of small-molecule bioanalytical sample preparations to a new frontier.

    PubMed

    Li, Ming; Chou, Judy; Jing, Jing; Xu, Hui; Costa, Aldo; Caputo, Robin; Mikkilineni, Rajesh; Flannelly-King, Shane; Rohde, Ellen; Gan, Lawrence; Klunk, Lewis; Yang, Liyu

    2012-06-01

    In recent years, there has been a growing interest in automating small-molecule bioanalytical sample preparations specifically using the Hamilton MicroLab(®) STAR liquid-handling platform. In the most extensive work reported thus far, multiple small-molecule sample preparation assay types (protein precipitation extraction, SPE and liquid-liquid extraction) have been integrated into a suite that is composed of graphical user interfaces and Hamilton scripts. Using that suite, bioanalytical scientists have been able to automate various sample preparation methods to a great extent. However, there are still areas that could benefit from further automation, specifically, the full integration of analytical standard and QC sample preparation with study sample extraction in one continuous run, real-time 2D barcode scanning on the Hamilton deck and direct Laboratory Information Management System database connectivity. We developed a new small-molecule sample-preparation automation system that improves in all of the aforementioned areas. The improved system presented herein further streamlines the bioanalytical workflow, simplifies batch run design, reduces analyst intervention and eliminates sample-handling error.

  13. The European Medicines Agency experience with biomarker qualification.

    PubMed

    Manolis, Efthymios; Koch, Armin; Deforce, Dieter; Vamvakas, Spiros

    2015-01-01

    Since the launch of the qualification process in 2009, the CHMP reviewed/is reviewing 48 requests for qualification advice or opinion (as of Sept 2013) related to biomarkers (BM) or other novel drug development tools (e.g. patient reported outcome measures, modeling, and statistical methods). The qualification opinions are available on the EMA website (Qualification of novel methodologies for medicine development, http://www.ema.europa.eu/ema/index.jsp?curl=pages/regulation/document_listing/document_listing_000319.jsp&mid=WC0b01ac0580022bb0#section2 , 2013). Also there is a trend of increasing numbers of qualification requests to CHMP, indicative of the pace that targeted drug development and personalized medicine is gaining and the need to bring the new tools from research to drug development and clinical use. This chapter will focus on the regulatory experience gained so far from the CHMP qualification procedure. Basic qualification principles will be presented. Through qualification examples, we will elaborate on common grounds and divergences between the different stakeholders.

  14. Sample preparation techniques for the determination of trace residues and contaminants in foods.

    PubMed

    Ridgway, Kathy; Lalljie, Sam P D; Smith, Roger M

    2007-06-15

    The determination of trace residues and contaminants in complex matrices, such as food, often requires extensive sample extraction and preparation prior to instrumental analysis. Sample preparation is often the bottleneck in analysis and there is a need to minimise the number of steps to reduce both time and sources of error. There is also a move towards more environmentally friendly techniques, which use less solvent and smaller sample sizes. Smaller sample size becomes important when dealing with real life problems, such as consumer complaints and alleged chemical contamination. Optimal sample preparation can reduce analysis time, sources of error, enhance sensitivity and enable unequivocal identification, confirmation and quantification. This review considers all aspects of sample preparation, covering general extraction techniques, such as Soxhlet and pressurised liquid extraction, microextraction techniques such as liquid phase microextraction (LPME) and more selective techniques, such as solid phase extraction (SPE), solid phase microextraction (SPME) and stir bar sorptive extraction (SBSE). The applicability of each technique in food analysis, particularly for the determination of trace organic contaminants in foods is discussed.

  15. Preparation of bone samples in the Gliwice Radiocarbon Laboratory for AMS radiocarbon dating.

    PubMed

    Piotrowska, N; Goslar, T

    2002-12-01

    In the Gliwice Radiocarbon Laboratory, a system for preparation of samples for AMS dating has been built. At first it was used to produce graphite targets from plant macrofossils and sediments. In this study we extended its capabilities with the preparation of bones. We dealt with 3 methods; the first was the classical Longin method of collagen extraction, the second one included additional treatment of powdered bone in alkali solution, while in the third one carboxyl carbon was separated from amino acids obtained after hydrolysis of protein. The suitability of the methods was tested on 2 bone samples. Most of our samples gave ages > 40 kyr BP, suggesting good performance of the adapted methods, except for one sample prepared with simple Longin method. For routine preparation of bones we chose the Longin method with additional alkali treatment.

  16. Current trends and challenges in sample preparation for metallic nanoparticles analysis in daily products and environmental samples: A review

    NASA Astrophysics Data System (ADS)

    De la Calle, Inmaculada; Menta, Mathieu; Séby, Fabienne

    2016-11-01

    Due to the increasing use of nanoparticles (NPs) in consumer products, it becomes necessary to develop different strategies for their detection, identification, characterization and quantification in a wide variety of samples. Since the analysis of NPs in consumer products and environmental samples is particularly troublesome, a detailed description of challenges and limitations is given here. This review mainly focuses on sample preparation procedures applied for the mostly used techniques for metallic and metal oxide NPs characterization in consumer products and most outstanding publications of biological and environmental samples (from 2006 to 2015). We summarize the procedures applied for total metal content, extraction/separation and/or preconcentration of NPs from the matrix, separation of metallic NPs from their ions or from larger particles and NPs' size fractionation. Sample preparation procedures specifically for microscopy are also described. Selected applications in cosmetics, food, other consumer products, biological tissues and environmental samples are presented. Advantages and inconveniences of those procedures are considered. Moreover, selected simplified schemes for NPs sample preparation, as well as usual techniques applied are included. Finally, promising directions for further investigations are discussed.

  17. Novel sample preparation technique with needle-type micro-extraction device for volatile organic compounds in indoor air samples.

    PubMed

    Ueta, Ikuo; Mizuguchi, Ayako; Fujimura, Koji; Kawakubo, Susumu; Saito, Yoshihiro

    2012-10-09

    A novel needle-type sample preparation device was developed for the effective preconcentration of volatile organic compounds (VOCs) in indoor air before gas chromatography-mass spectrometry (GC-MS) analysis. To develop a device for extracting a wide range of VOCs typically found in indoor air, several types of particulate sorbents were tested as the extraction medium in the needle-type extraction device. To determine the content of these VOCs, air samples were collected for 30min with the packed sorbent(s) in the extraction needle, and the extracted VOCs were thermally desorbed in a GC injection port by the direct insertion of the needle. A double-bed sorbent consisting of a needle packed with divinylbenzene and activated carbon particles exhibited excellent extraction and desorption performance and adequate extraction capacity for all the investigated VOCs. The results also clearly demonstrated that the proposed sample preparation method is a more rapid, simpler extraction/desorption technique than traditional sample preparation methods. Copyright © 2012 Elsevier B.V. All rights reserved.

  18. 14 CFR 121.453 - Flight engineer qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Flight engineer qualifications. 121.453... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Crewmember Qualifications § 121.453 Flight engineer qualifications. (a) No certificate holder may use any person nor may any person serve as a flight engineer on an...

  19. 14 CFR 121.453 - Flight engineer qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Flight engineer qualifications. 121.453... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Crewmember Qualifications § 121.453 Flight engineer qualifications. (a) No certificate holder may use any person nor may any person serve as a flight engineer on an...

  20. 14 CFR 121.453 - Flight engineer qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Flight engineer qualifications. 121.453... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Crewmember Qualifications § 121.453 Flight engineer qualifications. (a) No certificate holder may use any person nor may any person serve as a flight engineer on an...

  1. 14 CFR 121.453 - Flight engineer qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Flight engineer qualifications. 121.453... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Crewmember Qualifications § 121.453 Flight engineer qualifications. (a) No certificate holder may use any person nor may any person serve as a flight engineer on an...

  2. 14 CFR 121.453 - Flight engineer qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Flight engineer qualifications. 121.453... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Crewmember Qualifications § 121.453 Flight engineer qualifications. (a) No certificate holder may use any person nor may any person serve as a flight engineer on an...

  3. 29 CFR 1926.1428 - Signal person qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 8 2012-07-01 2012-07-01 false Signal person qualifications. 1926.1428 Section 1926.1428... Construction § 1926.1428 Signal person qualifications. (a) The employer of the signal person must ensure that each signal person meets the Qualification Requirements (paragraph (c) of this section) prior to giving...

  4. 29 CFR 1926.1428 - Signal person qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 8 2014-07-01 2014-07-01 false Signal person qualifications. 1926.1428 Section 1926.1428... Construction § 1926.1428 Signal person qualifications. (a) The employer of the signal person must ensure that each signal person meets the Qualification Requirements (paragraph (c) of this section) prior to giving...

  5. 29 CFR 1926.1428 - Signal person qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 8 2013-07-01 2013-07-01 false Signal person qualifications. 1926.1428 Section 1926.1428... Construction § 1926.1428 Signal person qualifications. (a) The employer of the signal person must ensure that each signal person meets the Qualification Requirements (paragraph (c) of this section) prior to giving...

  6. 32 CFR 171.4 - Qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 1 2014-07-01 2014-07-01 false Qualifications. 171.4 Section 171.4 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DEFENSE CONTRACTING IMPLEMENTATION OF WILDFIRE SUPPRESSION AIRCRAFT TRANSFER ACT OF 1996 § 171.4 Qualifications. The Secretary of Agriculture...

  7. 32 CFR 171.4 - Qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 1 2013-07-01 2013-07-01 false Qualifications. 171.4 Section 171.4 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DEFENSE CONTRACTING IMPLEMENTATION OF WILDFIRE SUPPRESSION AIRCRAFT TRANSFER ACT OF 1996 § 171.4 Qualifications. The Secretary of Agriculture...

  8. 32 CFR 171.4 - Qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 1 2012-07-01 2012-07-01 false Qualifications. 171.4 Section 171.4 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DEFENSE CONTRACTING IMPLEMENTATION OF WILDFIRE SUPPRESSION AIRCRAFT TRANSFER ACT OF 1996 § 171.4 Qualifications. The Secretary of Agriculture...

  9. 32 CFR 171.4 - Qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 1 2011-07-01 2011-07-01 false Qualifications. 171.4 Section 171.4 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DEFENSE CONTRACTING IMPLEMENTATION OF WILDFIRE SUPPRESSION AIRCRAFT TRANSFER ACT OF 1996 § 171.4 Qualifications. The Secretary of Agriculture...

  10. 42 CFR 61.5 - Qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 1 2013-10-01 2013-10-01 false Qualifications. 61.5 Section 61.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Regular Fellowships § 61.5 Qualifications. In order to qualify for a regular fellowship, an...

  11. 42 CFR 61.5 - Qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Qualifications. 61.5 Section 61.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Regular Fellowships § 61.5 Qualifications. In order to qualify for a regular fellowship, an...

  12. 42 CFR 61.5 - Qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false Qualifications. 61.5 Section 61.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Regular Fellowships § 61.5 Qualifications. In order to qualify for a regular fellowship, an...

  13. 42 CFR 61.5 - Qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Qualifications. 61.5 Section 61.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Regular Fellowships § 61.5 Qualifications. In order to qualify for a regular fellowship, an...

  14. 42 CFR 61.5 - Qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false Qualifications. 61.5 Section 61.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING FELLOWSHIPS Regular Fellowships § 61.5 Qualifications. In order to qualify for a regular fellowship, an...

  15. Coleman prepares to insert samples into the MELFI

    NASA Image and Video Library

    2010-12-27

    ISS026-E-023768 (27 Dec. 2010) --- NASA astronaut Catherine (Cady) Coleman, Expedition 26 flight engineer, prepares to insert samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

  16. Sample Preparation of Corn Seed Tissue to Prevent Analyte Relocations for Mass Spectrometry Imaging

    NASA Astrophysics Data System (ADS)

    Kim, Shin Hye; Kim, Jeongkwon; Lee, Young Jin; Lee, Tae Geol; Yoon, Sohee

    2017-08-01

    Corn seed tissue sections were prepared by the tape support method using an adhesive tape, and mass spectrometry imaging (MSI) was performed. The effect of heat generated during sample preparation was investigated by time-of-flight secondary mass spectrometry (TOF-SIMS) imaging of corn seed tissue prepared by the tape support and the thaw-mounted methods. Unlike thaw-mounted sample preparation, the tape support method does not cause imaging distortion because of the absence of heat, which can cause migration of the analytes on the sample. By applying the tape-support method, the corn seed tissue was prepared without structural damage and MSI with accurate spatial information of analytes was successfully performed.

  17. 46 CFR 56.70-20 - Qualification, general.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Qualification, general. 56.70-20 Section 56.70-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Fabrication, Assembly and Erection § 56.70-20 Qualification, general. (a) Qualification of the welding procedures to be used, and of the...

  18. 46 CFR 56.70-20 - Qualification, general.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 2 2013-10-01 2013-10-01 false Qualification, general. 56.70-20 Section 56.70-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Fabrication, Assembly and Erection § 56.70-20 Qualification, general. (a) Qualification of the welding procedures to be used, and of the...

  19. 46 CFR 56.75-20 - Brazing qualification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 2 2014-10-01 2014-10-01 false Brazing qualification. 56.75-20 Section 56.75-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-20 Brazing qualification. (a) The qualification of the performance of brazers and brazing operators shall be in accordance...

  20. 46 CFR 56.70-20 - Qualification, general.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 2 2014-10-01 2014-10-01 false Qualification, general. 56.70-20 Section 56.70-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Fabrication, Assembly and Erection § 56.70-20 Qualification, general. (a) Qualification of the welding procedures to be used, and of the...

  1. 46 CFR 56.70-20 - Qualification, general.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 2 2012-10-01 2012-10-01 false Qualification, general. 56.70-20 Section 56.70-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Fabrication, Assembly and Erection § 56.70-20 Qualification, general. (a) Qualification of the welding procedures to be used, and of the...

  2. 46 CFR 56.70-20 - Qualification, general.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 2 2011-10-01 2011-10-01 false Qualification, general. 56.70-20 Section 56.70-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Fabrication, Assembly and Erection § 56.70-20 Qualification, general. (a) Qualification of the welding procedures to be used, and of the...

  3. 46 CFR 56.75-20 - Brazing qualification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 2 2012-10-01 2012-10-01 false Brazing qualification. 56.75-20 Section 56.75-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-20 Brazing qualification. (a) The qualification of the performance of brazers and brazing operators shall be in accordance...

  4. 46 CFR 56.75-20 - Brazing qualification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 2 2013-10-01 2013-10-01 false Brazing qualification. 56.75-20 Section 56.75-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-20 Brazing qualification. (a) The qualification of the performance of brazers and brazing operators shall be in accordance...

  5. 46 CFR 56.75-20 - Brazing qualification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Brazing qualification. 56.75-20 Section 56.75-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-20 Brazing qualification. (a) The qualification of the performance of brazers and brazing operators shall be in accordance...

  6. 46 CFR 56.75-20 - Brazing qualification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 2 2011-10-01 2011-10-01 false Brazing qualification. 56.75-20 Section 56.75-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-20 Brazing qualification. (a) The qualification of the performance of brazers and brazing operators shall be in accordance...

  7. Apparatus for preparing a sample for mass spectrometry

    DOEpatents

    Villa-Aleman, Eliel

    1994-01-01

    An apparatus for preparing a sample for analysis by a mass spectrometer system. The apparatus has an entry chamber and an ionization chamber separated by a skimmer. A capacitor having two space-apart electrodes followed by one or more ion-imaging lenses is disposed in the ionization chamber. The chamber is evacuated and the capacitor is charged. A valve injects a sample gas in the form of sample pulses into the entry chamber. The pulse is collimated by the skimmer and enters the ionization chamber. When the sample pulse passes through the gap between the electrodes, it discharges the capacitor and is thereby ionized. The ions are focused by the imaging lenses and enter the mass analyzer, where their mass and charge are analyzed.

  8. Unintended Consequences: How Qualification Constrains Innovation

    NASA Technical Reports Server (NTRS)

    Brice, Craig A.

    2011-01-01

    The development and implementation of new materials and manufacturing processes for aerospace application is often hindered by the high cost and long time span associated with current qualification procedures. The data requirements necessary for material and process qualification are extensive and often require millions of dollars and multiple years to complete. Furthermore, these qualification data can become obsolete for even minor changes to the processing route. This burden is a serious impediment to the pursuit of revolutionary new materials and more affordable processing methods for air vehicle structures. The application of integrated computational materials engineering methods to this problem can help to reduce the barriers to rapid insertion of new materials and processes. By establishing predictive capability for the development of microstructural features in relation to processing and relating this to critical property characteristics, a streamlined approach to qualification is possible. This paper critically examines the advantages and challenges to a modeling-assisted qualification approach for aerospace structural materials. An example of how this approach might apply towards the emerging field of additive manufacturing is discussed in detail.

  9. Establishment of an unrelated umbilical cord blood bank qualification program: ensuring quality while meeting Food and Drug Administration vendor qualification requirements.

    PubMed

    Rabe, Fran; Kadidlo, Diane; Van Orsow, Lisa; McKenna, David

    2013-10-01

    Qualification of a cord blood bank (CBB) is a complex process that includes evaluation of multiple aspects of donor screening and testing, processing, accreditation and approval by professional cell therapy groups, and results of received cord blood units. The University of Minnesota Medical Center Cell Therapy Laboratory has established a CBB vendor qualification process to ensure the CBB meets established regulatory and quality requirements. The deployed qualification of CBBs is based on retrospective and prospective review of the CBB. Forty-one CBBs were evaluated retrospectively: seven CBBs were disqualified based on failed quality control (QC) results. Eight CBBs did not meet the criteria for retrospective qualification because fewer than 3 cord blood units were received and the CBB was not accredited. As of March 2012, three US and one non-US CBBs have been qualified prospectively. One CBB withdrew from the qualification process after successful completion of the comprehensive survey and subsequent failure of the provided QC unit to pass the minimum criteria. One CBB failed the prospective qualification process based on processing methods that were revealed during the paper portion of the evaluation. A CBB qualification process is necessary for a transplant center to manage the qualification of the large number of CBBs needed to support a umbilical cord blood transplantation program. A transplant center that has utilized cord blood for a number of years before implementation of a qualification process should use a retrospective qualification process along with a prospective process. © 2013 American Association of Blood Banks.

  10. School Mathematics Qualifications and Work

    ERIC Educational Resources Information Center

    Drake, Pat; Noyes, Andrew; Wake, Geoff

    2012-01-01

    This article explores the relationship between school mathematics qualifications and the transition into employment. As part of a large-scale, three-year independent evaluation of pilot qualifications in 14-19 school mathematics, all 39 Sector Skills Councils and Employment Skills Boards in the UK were invited to provide evidence regarding their…

  11. Recent developments in sample preparation and data pre-treatment in metabonomics research.

    PubMed

    Li, Ning; Song, Yi peng; Tang, Huiru; Wang, Yulan

    2016-01-01

    Metabonomics is a powerful approach for biomarker discovery and an effective tool for pinpointing endpoint metabolic effects of external stimuli, such as pathogens and disease development. Due to its wide applications, metabonomics is required to deal with various biological samples of different properties. Hence sample preparation and corresponding data pre-treatment become important factors in ensuring validity of an investigation. In this review, we summarize some recent developments in metabonomics sample preparation and data-pretreatment procedures. Copyright © 2015 Elsevier Inc. All rights reserved.

  12. Anaerobic Capacity Changes of the National Freestyle Wrestlers during the Olympic Qualification Competition Period

    ERIC Educational Resources Information Center

    Sever, Ozan; Gönülates, Süleyman; Bayrakdar, Akan; Demirhan, Bilal; Geri, Serdar; Zorba, Erdal

    2017-01-01

    This study aimed to evaluate the national level wrestlers' anaerobic capacity through the preparation and qualification periods for Rio 2016 summer Olympic Games. For this manner, 10 national level freestyle wrestlers' (age 22,10 ± 3,21; weight 64,75 ± 6,34; height 164,31 ± 4,75) anaerobic outputs measured 3 times in three month intervals with…

  13. Characterization of renal biomarkers for use in clinical trials: effect of preanalytical processing and qualification using samples from subjects with diabetes.

    PubMed

    Brott, David A; Furlong, Stephen T; Adler, Scott H; Hainer, James W; Arani, Ramin B; Pinches, Mark; Rossing, Peter; Chaturvedi, Nish

    2015-01-01

    Identifying the potential for drug-induced kidney injury is essential for the successful research and development of new drugs. Newer and more sensitive preclinical drug-induced kidney injury biomarkers are now qualified for use in rat toxicology studies, but biomarkers for clinical studies are still undergoing qualification. The current studies investigated biomarkers in healthy volunteer (HV) urine samples with and without the addition of stabilizer as well as in urine from patients with normoalbuminuric diabetes mellitus (P-DM). Urine samples from 20 male HV with stabilizer, 69 male HV without stabilizer, and 95 male DM without stabilizer (39 type 1 and 56 type 2) were analyzed for the following bio-markers using multiplex assays: α-1-microglobulin (A1M), β-2-microglobulin, calbindin, clusterin, connective tissue growth factor (CTGF), creatinine, cystatin-C, glutathione S-transferase α (GSTα), kidney injury marker-1 (KIM-1), microalbumin, neutrophil gelatinase-associated lipocalin, osteopontin, Tamm-Horsfall urinary glycoprotein (THP), tissue inhibitor of metalloproteinase 1, trefoil factor 3 (TFF3), and vascular endothelial growth factor. CTGF and GSTα assays on nonstabilized urine were deemed nonoptimal (>50% of values below assay lower limits of quantification). "Expected values" were determined for HV with stabilizer, HV without stabilizer, and P-DM without stabilizer. There was a statistically significant difference between HV with stabilizer compared to HV without stabilizer for A1M, CTGF, GSTα, and THP. DM urine samples differed from HV (without stabilizer) for A1M CTGF, GSTα, KIM-1, microalbumin, osteopontin, and TFF3. A1M also correctly identified HV and DM with an accuracy of 89.0%. These studies: 1) determined that nonstabilized urine can be used for assays under qualification; and 2) documented that A1M, CTGF, GSTα, KIM-1, microalbumin, osteopontin, and TFF3 were significantly increased in urine from P-DM. In addition, the 89.0% accuracy of A

  14. Round table discussion " Development of qualification framework in meteorology (TEMPUS QUALIMET)"

    NASA Astrophysics Data System (ADS)

    Bashmakova, I.; Belotserkovsky, A.; Karlin, L.; Petrosyan, A.; Serditova, N.; Zilitinkevich, S.

    2010-09-01

    The international consortium has started implementing a project aimed at the development of unified framework of qualifications in meteorology (QualiMet), setting a system of recognition and award of qualifications up to Doctoral level based on standards of knowledge, skill and competence acquired by learners is underway. The QualiMet has the following specific objectives: 1. To develop standards of knowledge, skills and competence for all qualifications up to Doctoral level needed in all possible occupations meteorology learner can undertake, by July 2011 2. To develop reciprocally recognized rubrics, criteria, methods and tools for assessing the compliance with the developed standards (quality assurance), by July 2012 3. To set the network of Centers of Excellence as the primary designer of sample education programs and learning experiences, both in brick-and-mortar and distant setting of delivery, leading to achievement of the developed standards, by December 2012 4. To set a system of mutual international recognition and award of qualifications in meteorology based on the developed procedures and establishment of self-regulatory public organization, by December 2012 The main beneficiaries of the project are: 1. Meteorology learners from the consortium countries. They will be able to make informed decisions about available qualification choices and progression options and provided an opportunity for students and graduates to participate in the system of international continuous education. 2. Meteorology employers from the consortium countries, They will be able to specify the level of knowledge, skill and competence required for occupational roles, evaluate qualifications presented, connect training and development with business needs. 3. Students and academic staff of all the consortium members, who will gain the increased mobility and exchange the fluxes of culturally and institutionally diversified lecturers and qualified specialists

  15. Sample preparation and detection device for infectious agents

    DOEpatents

    Miles, Robin R.; Wang, Amy W.; Fuller, Christopher K.; Lemoff, Asuncion V.; Bettencourt, Kerry A.; Yu, June

    2003-06-10

    A sample preparation and analysis device which incorporates both immunoassays and PCR assays in one compact, field-portable microchip. The device provides new capabilities in fluid and particle control which allows the building of a fluidic chip with no moving parts, thus decreasing fabrication cost and increasing the robustness of the device. The device can operate in a true continuous (not batch) mode. The device incorporates magnetohydrodynamic (MHD) pumps to move the fluid through the system, acoustic mixing and fractionation, dielectropheretic (DEP) sample concentration and purification, and on-chip optical detection capabilities.

  16. Solventless and solvent-minimized sample preparation techniques for determining currently used pesticides in water samples: a review.

    PubMed

    Tankiewicz, Maciej; Fenik, Jolanta; Biziuk, Marek

    2011-10-30

    The intensification of agriculture means that increasing amounts of toxic organic and inorganic compounds are entering the environment. The pesticides generally applied nowadays are regarded as some of the most dangerous contaminants of the environment. Their presence in the environment, especially in water, is hazardous because they cause human beings to become more susceptible to disease. For these reasons, it is essential to monitor pesticide residues in the environment with the aid of all accessible analytical methods. The analysis of samples for the presence of pesticides is problematic, because of the laborious and time-consuming operations involved in preparing samples for analysis, which themselves may be a source of additional contaminations and errors. To date, it has been standard practice to use large quantities of organic solvents in the sample preparation process; but as these solvents are themselves hazardous, solventless and solvent-minimized techniques are coming into use. This paper discusses the most commonly used over the last 15 years sample preparation techniques for monitoring organophosphorus and organonitrogen pesticides residue in water samples. Furthermore, a significant trend in sample preparation, in accordance with the principles of 'Green Chemistry' is the simplification, miniaturization and automation of analytical techniques. In view of this aspect, several novel techniques are being developed in order to reduce the analysis step, increase the sample throughput and to improve the quality and the sensitivity of analytical methods. The paper describes extraction techniques requiring the use of solvents - liquid-liquid extraction (LLE) and its modifications, membrane extraction techniques, hollow fibre-protected two-phase solvent microextraction, liquid phase microextraction based on the solidification of a floating organic drop (LPME-SFO), solid-phase extraction (SPE) and single-drop microextraction (SDME) - as well as solvent

  17. Apparatus for preparing a sample for mass spectrometry

    DOEpatents

    Villa-Aleman, E.

    1994-05-10

    An apparatus is described for preparing a sample for analysis by a mass spectrometer system. The apparatus has an entry chamber and an ionization chamber separated by a skimmer. A capacitor having two space-apart electrodes followed by one or more ion-imaging lenses is disposed in the ionization chamber. The chamber is evacuated and the capacitor is charged. A valve injects a sample gas in the form of sample pulses into the entry chamber. The pulse is collimated by the skimmer and enters the ionization chamber. When the sample pulse passes through the gap between the electrodes, it discharges the capacitor and is thereby ionized. The ions are focused by the imaging lenses and enter the mass analyzer, where their mass and charge are analyzed. 1 figures.

  18. From Old to New: The Australian Qualifications Framework

    ERIC Educational Resources Information Center

    Wheelahan, Leesa

    2011-01-01

    The Australian Qualifications Framework (AQF) is a "first generation" qualifications framework that was established in 1995. Its purpose was to create "a comprehensive, nationally consistent yet flexible framework for all qualifications in post-compulsory education and training." It encompasses all post-compulsory…

  19. 10 CFR 26.85 - Collector qualifications and responsibilities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Collector qualifications and responsibilities. 26.85 Section 26.85 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Collecting Specimens for Testing § 26.85 Collector qualifications and responsibilities. (a) Urine collector qualifications. Urine...

  20. 10 CFR 26.85 - Collector qualifications and responsibilities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Collector qualifications and responsibilities. 26.85 Section 26.85 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Collecting Specimens for Testing § 26.85 Collector qualifications and responsibilities. (a) Urine collector qualifications. Urine...

  1. 10 CFR 26.85 - Collector qualifications and responsibilities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Collector qualifications and responsibilities. 26.85 Section 26.85 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Collecting Specimens for Testing § 26.85 Collector qualifications and responsibilities. (a) Urine collector qualifications. Urine...

  2. 10 CFR 26.85 - Collector qualifications and responsibilities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Collector qualifications and responsibilities. 26.85 Section 26.85 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Collecting Specimens for Testing § 26.85 Collector qualifications and responsibilities. (a) Urine collector qualifications. Urine...

  3. 10 CFR 26.85 - Collector qualifications and responsibilities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Collector qualifications and responsibilities. 26.85 Section 26.85 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Collecting Specimens for Testing § 26.85 Collector qualifications and responsibilities. (a) Urine collector qualifications. Urine...

  4. The Level of Diagnostic Tests' Preparation Skills among the Teachers of the First Three Elementary Grades' Teachers at the Directorate of Education of Bani Kinana District

    ERIC Educational Resources Information Center

    Yaghmour, Kholoud Subhi; Obaidat, Luai Taleb; Hamadneh, Qaseem Mohammad

    2016-01-01

    This study aimed at exploring the level of Diagnostic Tests' Preparation Skills among the teachers of the first Three Elementary Grades at the Directorate of Education of Bani Kinana District, and its relationship to the variables of gender, academic qualification, and years of experience. The sample of the study consisted of (264) male and female…

  5. 9 CFR 147.8 - Procedures for preparing egg yolk samples for diagnostic tests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Procedures for preparing egg yolk samples for diagnostic tests. 147.8 Section 147.8 Animals and Animal Products ANIMAL AND PLANT HEALTH... IMPROVEMENT PLAN Blood Testing Procedures § 147.8 Procedures for preparing egg yolk samples for diagnostic...

  6. 9 CFR 147.8 - Procedures for preparing egg yolk samples for diagnostic tests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Procedures for preparing egg yolk samples for diagnostic tests. 147.8 Section 147.8 Animals and Animal Products ANIMAL AND PLANT HEALTH... IMPROVEMENT PLAN Blood Testing Procedures § 147.8 Procedures for preparing egg yolk samples for diagnostic...

  7. 9 CFR 147.8 - Procedures for preparing egg yolk samples for diagnostic tests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Procedures for preparing egg yolk samples for diagnostic tests. 147.8 Section 147.8 Animals and Animal Products ANIMAL AND PLANT HEALTH... IMPROVEMENT PLAN Blood Testing Procedures § 147.8 Procedures for preparing egg yolk samples for diagnostic...

  8. 9 CFR 147.8 - Procedures for preparing egg yolk samples for diagnostic tests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Procedures for preparing egg yolk samples for diagnostic tests. 147.8 Section 147.8 Animals and Animal Products ANIMAL AND PLANT HEALTH... IMPROVEMENT PLAN Blood Testing Procedures § 147.8 Procedures for preparing egg yolk samples for diagnostic...

  9. 48 CFR 1553.209 - Contractor qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contractor qualifications. 1553.209 Section 1553.209 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION AGENCY CLAUSES AND FORMS FORMS Prescription of Forms 1553.209 Contractor qualifications. ...

  10. Qualifications Mismatch and Skills Mismatch

    ERIC Educational Resources Information Center

    Sutherland, John

    2012-01-01

    Purpose: The purpose of this paper is to estimate the extent of "over-qualification" (i.e. holding a qualification which is above that required to gain entry to the job being done) and "skills under-utilisation" (i.e. being in a job which does not make use of the knowledge and skills possessed) in the United Kingdom and to…

  11. 28 CFR 34.109 - Qualifications of peer reviewers.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 34.109 Judicial Administration DEPARTMENT OF JUSTICE OJJDP COMPETITION AND PEER REVIEW PROCEDURES Peer Review § 34.109 Qualifications of peer reviewers. The general reviewer qualification criteria to...). Additional details concerning peer reviewer qualifications are provided in the OJJDP “Peer Review Guideline”. ...

  12. 28 CFR 50.24 - Annuity broker minimum qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Annuity broker minimum qualifications. 50....24 Annuity broker minimum qualifications. (a) Minimum standards. The Civil Division, United States Department of Justice, shall establish a list of annuity brokers who meet minimum qualifications for...

  13. 5 CFR 317.502 - Qualifications Review Board certification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS EMPLOYMENT IN THE SENIOR EXECUTIVE SERVICE Career Appointments § 317.502 Qualifications Review Board certification. (a) A Qualification Review Board (QRB) convened by OPM must certify the executive... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Qualifications Review Board certification...

  14. High-resolution X-ray diffraction with no sample preparation

    PubMed Central

    Turner, S. M. R.; Degryse, P.; Shortland, A. J.

    2017-01-01

    It is shown that energy-dispersive X-ray diffraction (EDXRD) implemented in a back-reflection geometry is extremely insensitive to sample morphology and positioning even in a high-resolution configuration. This technique allows high-quality X-ray diffraction analysis of samples that have not been prepared and is therefore completely non-destructive. The experimental technique was implemented on beamline B18 at the Diamond Light Source synchrotron in Oxfordshire, UK. The majority of the experiments in this study were performed with pre-characterized geological materials in order to elucidate the characteristics of this novel technique and to develop the analysis methods. Results are presented that demonstrate phase identification, the derivation of precise unit-cell parameters and extraction of microstructural information on unprepared rock samples and other sample types. A particular highlight was the identification of a specific polytype of a muscovite in an unprepared mica schist sample, avoiding the time-consuming and difficult preparation steps normally required to make this type of identification. The technique was also demonstrated in application to a small number of fossil and archaeological samples. Back-reflection EDXRD implemented in a high-resolution configuration shows great potential in the crystallographic analysis of cultural heritage artefacts for the purposes of scientific research such as provenancing, as well as contributing to the formulation of conservation strategies. Possibilities for moving the technique from the synchrotron into museums are discussed. The avoidance of the need to extract samples from high-value and rare objects is a highly significant advantage, applicable also in other potential research areas such as palaeontology, and the study of meteorites and planetary materials brought to Earth by sample-return missions. PMID:28660862

  15. 48 CFR 53.209 - Contractor qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Contractor qualifications. 53.209 Section 53.209 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS FORMS Prescription of Forms 53.209 Contractor qualifications. ...

  16. Potential artifacts associated with historical preparation of joint compound samples and reported airborne asbestos concentrations.

    PubMed

    Brorby, G P; Sheehan, P J; Berman, D W; Bogen, K T; Holm, S E

    2011-05-01

    Airborne samples collected in the 1970s for drywall workers using asbestos-containing joint compounds were likely prepared and analyzed according to National Institute of Occupational Safety and Health Method P&CAM 239, the historical precursor to current Method 7400. Experimentation with a re-created, chrysotile-containing, carbonate-based joint compound suggested that analysis following sample preparation by the historical vs. current method produces different fiber counts, likely because of an interaction between the different clearing and mounting chemicals used and the carbonate-based joint compound matrix. Differences were also observed during analysis using Method 7402, depending on whether acetic acid/dimethylformamide or acetone was used during preparation to collapse the filter. Specifically, air samples of sanded chrysotile-containing joint compound prepared by the historical method yielded fiber counts significantly greater (average of 1.7-fold, 95% confidence interval: 1.5- to 2.0-fold) than those obtained by the current method. In addition, air samples prepared by Method 7402 using acetic acid/dimethylformamide yielded fiber counts that were greater (2.8-fold, 95% confidence interval: 2.5- to 3.2-fold) than those prepared by this method using acetone. These results indicated (1) there is an interaction between Method P&CAM 239 preparation chemicals and the carbonate-based joint compound matrix that reveals fibers that were previously bound in the matrix, and (2) the same appeared to be true for Method 7402 preparation chemicals acetic acid/dimethylformamide. This difference in fiber counts is the opposite of what has been reported historically for samples of relatively pure chrysotile dusts prepared using the same chemicals. This preparation artifact should be considered when interpreting historical air samples for drywall workers prepared by Method P&CAM 239. Copyright © 2011 JOEH, LLC

  17. 40 CFR 761.392 - Preparing validation study samples.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... establish a surface concentration to be included in the standard operating procedure. The surface levels of... Under § 761.79(d)(4) § 761.392 Preparing validation study samples. (a)(1) To validate a procedure to... surfaces must be ≥20 µg/100 cm2. (2) To validate a procedure to decontaminate a specified surface...

  18. 40 CFR 761.392 - Preparing validation study samples.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... establish a surface concentration to be included in the standard operating procedure. The surface levels of... Under § 761.79(d)(4) § 761.392 Preparing validation study samples. (a)(1) To validate a procedure to... surfaces must be ≥20 µg/100 cm2. (2) To validate a procedure to decontaminate a specified surface...

  19. 40 CFR 761.392 - Preparing validation study samples.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... establish a surface concentration to be included in the standard operating procedure. The surface levels of... Under § 761.79(d)(4) § 761.392 Preparing validation study samples. (a)(1) To validate a procedure to... surfaces must be ≥20 µg/100 cm2. (2) To validate a procedure to decontaminate a specified surface...

  20. Advanced Curation Preparation for Mars Sample Return and Cold Curation

    NASA Technical Reports Server (NTRS)

    Fries, M. D.; Harrington, A. D.; McCubbin, F. M.; Mitchell, J.; Regberg, A. B.; Snead, C.

    2017-01-01

    NASA Curation is tasked with the care and distribution of NASA's sample collections, such as the Apollo lunar samples and cometary material collected by the Stardust spacecraft. Curation is also mandated to perform Advanced Curation research and development, which includes improving the curation of existing collections as well as preparing for future sample return missions. Advanced Curation has identified a suite of technologies and techniques that will require attention ahead of Mars sample return (MSR) and missions with cold curation (CCur) requirements, perhaps including comet sample return missions.

  1. 14 CFR 121.439 - Pilot qualification: Recent experience.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Pilot qualification: Recent experience. 121... Pilot qualification: Recent experience. Link to an amendment published at 78 FR 67841, Nov. 12, 2013. (a... qualification: Recent experience. (f) Deviation authority based upon designation of related aircraft in...

  2. An efficient and cost-effective method for preparing transmission electron microscopy samples from powders

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wen, Haiming; Lin, Yaojun; Seidman, David N.

    The preparation of transmission electron microcopy (TEM) samples from powders with particle sizes larger than ~100 nm poses a challenge. The existing methods are complicated and expensive, or have a low probability of success. Herein, we report a modified methodology for preparation of TEM samples from powders, which is efficient, cost-effective, and easy to perform. This method involves mixing powders with an epoxy on a piece of weighing paper, curing the powder–epoxy mixture to form a bulk material, grinding the bulk to obtain a thin foil, punching TEM discs from the foil, dimpling the discs, and ion milling the dimpledmore » discs to electron transparency. Compared with the well established and robust grinding–dimpling–ion-milling method for TEM sample preparation for bulk materials, our modified approach for preparing TEM samples from powders only requires two additional simple steps. In this article, step-by-step procedures for our methodology are described in detail, and important strategies to ensure success are elucidated. Furthermore, our methodology has been applied successfully for preparing TEM samples with large thin areas and high quality for many different mechanically milled metallic powders.« less

  3. An efficient and cost-effective method for preparing transmission electron microscopy samples from powders

    DOE PAGES

    Wen, Haiming; Lin, Yaojun; Seidman, David N.; ...

    2015-09-09

    The preparation of transmission electron microcopy (TEM) samples from powders with particle sizes larger than ~100 nm poses a challenge. The existing methods are complicated and expensive, or have a low probability of success. Herein, we report a modified methodology for preparation of TEM samples from powders, which is efficient, cost-effective, and easy to perform. This method involves mixing powders with an epoxy on a piece of weighing paper, curing the powder–epoxy mixture to form a bulk material, grinding the bulk to obtain a thin foil, punching TEM discs from the foil, dimpling the discs, and ion milling the dimpledmore » discs to electron transparency. Compared with the well established and robust grinding–dimpling–ion-milling method for TEM sample preparation for bulk materials, our modified approach for preparing TEM samples from powders only requires two additional simple steps. In this article, step-by-step procedures for our methodology are described in detail, and important strategies to ensure success are elucidated. Furthermore, our methodology has been applied successfully for preparing TEM samples with large thin areas and high quality for many different mechanically milled metallic powders.« less

  4. On the use of ultracentrifugal devices for routine sample preparation in biomolecular magic-angle-spinning NMR

    PubMed Central

    Mandal, Abhishek; Boatz, Jennifer C.; Wheeler, Travis; van der Wel, Patrick C. A.

    2017-01-01

    A number of recent advances in the field of magic-angle-spinning (MAS) solid-state NMR have enabled its application to a range of biological systems of ever increasing complexity. To retain biological relevance, these samples are increasingly studied in a hydrated state. At the same time, experimental feasibility requires the sample preparation process to attain a high sample concentration within the final MAS rotor. We discuss these considerations, and how they have led to a number of different approaches to MAS NMR sample preparation. We describe our experience of how custom-made (or commercially available) ultracentrifugal devices can facilitate a simple, fast and reliable sample preparation process. A number of groups have since adopted such tools, in some cases to prepare samples for sedimentation-style MAS NMR experiments. Here we argue for a more widespread adoption of their use for routine MAS NMR sample preparation. PMID:28229262

  5. 49 CFR 180.603 - Qualification of portable tanks.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 3 2013-10-01 2013-10-01 false Qualification of portable tanks. 180.603 Section... MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Portable Tanks § 180.603 Qualification of portable tanks. (a) Each portable tank used for the transportation of hazardous materials must be an authorized...

  6. Unit Standards Catalogue. Unit Standards and Qualifications Registered on the National Qualifications Framework to March 1994.

    ERIC Educational Resources Information Center

    New Zealand Qualifications Authority, Wellington.

    This booklet includes the latest list of unit standards and qualifications registered on the New Zealand National Qualifications Framework to April 1994. Unit standards registered on the framework can be offered by private and government training establishments, polytechnics, colleges of education, and schools. This list of registered unit…

  7. 5 CFR 319.301 - Qualifications standards.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 1 2011-01-01 2011-01-01 false Qualifications standards. 319.301 Section... IN SENIOR-LEVEL AND SCIENTIFIC AND PROFESSIONAL POSITIONS Qualifications Requirements § 319.301... in the General Schedule. (c) Standards for scientific and professional positions. (1) Unless the...

  8. 5 CFR 319.301 - Qualifications standards.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Qualifications standards. 319.301 Section... IN SENIOR-LEVEL AND SCIENTIFIC AND PROFESSIONAL POSITIONS Qualifications Requirements § 319.301... in the General Schedule. (c) Standards for scientific and professional positions. (1) Unless the...

  9. 16 CFR 1210.14 - Qualification testing.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Qualification testing. 1210.14 Section 1210.14 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS SAFETY STANDARD FOR CIGARETTE LIGHTERS Certification Requirements § 1210.14 Qualification testing. (a...

  10. 5 CFR 330.709 - Qualification reviews.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Qualification reviews. 330.709 Section..., SELECTION, AND PLACEMENT (GENERAL) Interagency Career Transition Assistance Plan for Displaced Employees § 330.709 Qualification reviews. Agencies will ensure that a documented, independent second review is...

  11. 48 CFR 253.209 - Contractor qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Contractor qualifications. 253.209 Section 253.209 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS FORMS Prescription of Forms 253.209 Contractor qualifications. ...

  12. Qualification Procedures for VHSIC/VLSI

    DTIC Science & Technology

    1990-12-01

    alternative approach for qualification of complex microcircuits. To address the technical issues related to a process oriented qualification approach, the...methodology of microcircuit process control to promote the United States to a position of supplying the nighest quality and most reliable...available resources . o Coordinate document reviews with weekly and monthly status reviews on progress. o Summarize results and collate into four basic

  13. Cross-sectional TEM specimen preparation for W/B{sub 4}C multilayer sample using FIB

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mondal, Puspen, E-mail: puspen@rrcat.gov.in; Pradhan, P. C.; Tiwari, Pragya

    2016-05-23

    A recent emergence of a cross-beam scanning electron microscopy (SEM)/focused-ion-beam (FIB) system have given choice to fabricate cross-sectional transmission electron microscopy (TEM) specimen of thin film multilayer sample. A 300 layer pair thin film multilayer sample of W/B{sub 4}C was used to demonstrate the specimen lift-out technique in very short time as compared to conventional cross-sectional sample preparation technique. To get large area electron transparent sample, sample prepared by FIB is followed by Ar{sup +} ion polishing at 2 kV with grazing incident. The prepared cross-sectional sample was characterized by transmission electron microscope.

  14. Miniaturized sample preparation needle: a versatile design for the rapid analysis of smoking-related compounds in hair and air samples.

    PubMed

    Saito, Yoshihiro; Ueta, Ikuo; Ogawa, Mitsuhiro; Hayashida, Makiko; Jinno, Kiyokatsu

    2007-05-09

    Miniaturized needle extraction device has been developed as a versatile sample preparation device designed for the rapid and simple analysis of smoking-related compounds in smokers' hair samples and environmental tobacco smoke. Packed with polymeric particle, the resulting particle-packed needle was employed as a miniaturized sample preparation device for the analysis of typical volatile organic compounds in tobacco smoke. Introducing a bundle of polymer-coated filaments as the extraction medium, the needle was further applied as a novel sample preparation device containing simultaneous derivatization/extraction process of volatile aldehydes. Formaldehyde (FA) and acetaldehyde (AA) in smoker's breath during the smoking were successfully derivatized with two derivatization reagents in the polymer-coated fiber-packed needle device followed by the separation and determination in gas chromatography (GC). Smokers' hair samples were also packed into the needle, allowing the direct extraction of nicotine from the hair sample in a conventional GC injector. Optimizing the main experimental parameters for each technique, successful determination of several smoking-related compounds with these needle extraction methods has been demonstrated.

  15. An apparatus for preparing benthic samples aboard ship

    USGS Publications Warehouse

    Pepper, Phillip N.; Girard, Thomas L.; Stapanian, Martin A.

    2001-01-01

    We describe a safe and effective apparatus for washing and reducing the volume of benthic samples collected by grab samplers aboard ship. The sample is transferred directly from the dredge to the apparatus and then washed with water pumped through pipes in the apparatus and from onboard hoses. Wastewater and materials smaller than 0.541 mm in diameter are washed overboard. Larger materials, including benthic organisms, collect on an upper 0.64-cm screen and on a lower 30-mm-mesh stainless steel bolt cloth. A collection jar is screwed into the bottom of the apparatus. Therefore, transfer of sample material from the apparatus to the jar is quick and easy. This apparatus has several advantages for use aboard ship over others described in the literature, especially in rough seas, in cold weather, and at night. The apparatus provides a safe and convenient platform for washing and reducing samples, and samples can be prepared while the vessel is traveling at full speed.

  16. 28 CFR 115.402 - Auditor qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Auditor qualifications. 115.402 Section 115.402 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Auditing and Corrective Action § 115.402 Auditor qualifications. (a) An audit shall be conducted...

  17. 28 CFR 115.402 - Auditor qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Auditor qualifications. 115.402 Section 115.402 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Auditing and Corrective Action § 115.402 Auditor qualifications. (a) An audit shall be conducted...

  18. 28 CFR 115.402 - Auditor qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Auditor qualifications. 115.402 Section 115.402 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Auditing and Corrective Action § 115.402 Auditor qualifications. (a) An audit shall be conducted...

  19. 5 CFR 330.609 - Qualification reviews.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Qualification reviews. 330.609 Section..., SELECTION, AND PLACEMENT (GENERAL) Agency Career Transition Assistance Plans (CTAP) for Local Surplus and Displaced Employees § 330.609 Qualification reviews. Agencies will ensure that a documented, independent...

  20. Compact low temperature scanning tunneling microscope with in-situ sample preparation capability.

    PubMed

    Kim, Jungdae; Nam, Hyoungdo; Qin, Shengyong; Kim, Sang-ui; Schroeder, Allan; Eom, Daejin; Shih, Chih-Kang

    2015-09-01

    We report on the design of a compact low temperature scanning tunneling microscope (STM) having in-situ sample preparation capability. The in-situ sample preparation chamber was designed to be compact allowing quick transfer of samples to the STM stage, which is ideal for preparing temperature sensitive samples such as ultra-thin metal films on semiconductor substrates. Conventional spring suspensions on the STM head often cause mechanical issues. To address this problem, we developed a simple vibration damper consisting of welded metal bellows and rubber pads. In addition, we developed a novel technique to ensure an ultra-high-vacuum (UHV) seal between the copper and stainless steel, which provides excellent reliability for cryostats operating in UHV. The performance of the STM was tested from 2 K to 77 K by using epitaxial thin Pb films on Si. Very high mechanical stability was achieved with clear atomic resolution even when using cryostats operating at 77 K. At 2 K, a clean superconducting gap was observed, and the spectrum was easily fit using the BCS density of states with negligible broadening.

  1. Automated cellular sample preparation using a Centrifuge-on-a-Chip.

    PubMed

    Mach, Albert J; Kim, Jae Hyun; Arshi, Armin; Hur, Soojung Claire; Di Carlo, Dino

    2011-09-07

    The standard centrifuge is a laboratory instrument widely used by biologists and medical technicians for preparing cell samples. Efforts to automate the operations of concentration, cell separation, and solution exchange that a centrifuge performs in a simpler and smaller platform have had limited success. Here, we present a microfluidic chip that replicates the functions of a centrifuge without moving parts or external forces. The device operates using a purely fluid dynamic phenomenon in which cells selectively enter and are maintained in microscale vortices. Continuous and sequential operation allows enrichment of cancer cells from spiked blood samples at the mL min(-1) scale, followed by fluorescent labeling of intra- and extra-cellular antigens on the cells without the need for manual pipetting and washing steps. A versatile centrifuge-analogue may open opportunities in automated, low-cost and high-throughput sample preparation as an alternative to the standard benchtop centrifuge in standardized clinical diagnostics or resource poor settings.

  2. The Recognition of Occupational Skills and Educational Qualifications within the EEC.

    ERIC Educational Resources Information Center

    Bertrand, Olivier

    1992-01-01

    A survey conducted in France, Germany, Italy, Spain, and the United Kingdom analyzed employment practices and viewpoints of the social partners regarding comparability of qualifications and geographical mobility. Carried out in late 1991 with a limited sampling of respondents (firms, employers' organizations, and unions), the survey focused…

  3. 49 CFR 180.507 - Qualification of tank cars.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 3 2013-10-01 2013-10-01 false Qualification of tank cars. 180.507 Section 180... MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.507 Qualification of tank cars. (a) Each tank car marked as meeting a “DOT” specification or any other tank car used for the transportation...

  4. 49 CFR 180.507 - Qualification of tank cars.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 3 2014-10-01 2014-10-01 false Qualification of tank cars. 180.507 Section 180... MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.507 Qualification of tank cars. (a) Each tank car marked as meeting a “DOT” specification or any other tank car used for the transportation...

  5. 49 CFR 180.507 - Qualification of tank cars.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 3 2011-10-01 2011-10-01 false Qualification of tank cars. 180.507 Section 180... MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.507 Qualification of tank cars. (a) Each tank car marked as meeting a “DOT” specification or any other tank car used for the transportation...

  6. 49 CFR 180.507 - Qualification of tank cars.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 3 2012-10-01 2012-10-01 false Qualification of tank cars. 180.507 Section 180... MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.507 Qualification of tank cars. (a) Each tank car marked as meeting a “DOT” specification or any other tank car used for the transportation...

  7. 46 CFR 57.05-3 - Limited space qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 2 2012-10-01 2012-10-01 false Limited space qualifications. 57.05-3 Section 57.05-3... Performance Qualifications § 57.05-3 Limited space qualifications. When a welder is to be qualified for welding or torch brazing of piping on board ship in a limited or restricted space, the space restrictions...

  8. 46 CFR 57.05-3 - Limited space qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 2 2014-10-01 2014-10-01 false Limited space qualifications. 57.05-3 Section 57.05-3... Performance Qualifications § 57.05-3 Limited space qualifications. When a welder is to be qualified for welding or torch brazing of piping on board ship in a limited or restricted space, the space restrictions...

  9. 46 CFR 57.05-3 - Limited space qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 2 2011-10-01 2011-10-01 false Limited space qualifications. 57.05-3 Section 57.05-3... Performance Qualifications § 57.05-3 Limited space qualifications. When a welder is to be qualified for welding or torch brazing of piping on board ship in a limited or restricted space, the space restrictions...

  10. 46 CFR 57.05-3 - Limited space qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 2 2013-10-01 2013-10-01 false Limited space qualifications. 57.05-3 Section 57.05-3... Performance Qualifications § 57.05-3 Limited space qualifications. When a welder is to be qualified for welding or torch brazing of piping on board ship in a limited or restricted space, the space restrictions...

  11. 21 CFR 211.25 - Personnel qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 4 2014-04-01 2014-04-01 false Personnel qualifications. 211.25 Section 211.25 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS....25 Personnel qualifications. (a) Each person engaged in the manufacture, processing, packing, or...

  12. 21 CFR 211.25 - Personnel qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 4 2013-04-01 2013-04-01 false Personnel qualifications. 211.25 Section 211.25 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS....25 Personnel qualifications. (a) Each person engaged in the manufacture, processing, packing, or...

  13. 21 CFR 211.25 - Personnel qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 4 2012-04-01 2012-04-01 false Personnel qualifications. 211.25 Section 211.25 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS....25 Personnel qualifications. (a) Each person engaged in the manufacture, processing, packing, or...

  14. 34 CFR 300.156 - Personnel qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... children with disabilities. (c) Qualifications for special education teachers. The qualifications described in paragraph (a) of this section must ensure that each person employed as a public school special education teacher in the State who teaches in an elementary school, middle school, or secondary school is...

  15. 9 CFR 2.32 - Personnel qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Personnel qualifications. 2.32 Section 2.32 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Research Facilities § 2.32 Personnel qualifications. (a) It shall be...

  16. 9 CFR 2.32 - Personnel qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Personnel qualifications. 2.32 Section 2.32 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Research Facilities § 2.32 Personnel qualifications. (a) It shall be...

  17. 9 CFR 2.32 - Personnel qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Personnel qualifications. 2.32 Section 2.32 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Research Facilities § 2.32 Personnel qualifications. (a) It shall be...

  18. 9 CFR 2.32 - Personnel qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Personnel qualifications. 2.32 Section 2.32 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Research Facilities § 2.32 Personnel qualifications. (a) It shall be...

  19. 9 CFR 2.32 - Personnel qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Personnel qualifications. 2.32 Section 2.32 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Research Facilities § 2.32 Personnel qualifications. (a) It shall be...

  20. Ionic liquids: solvents and sorbents in sample preparation.

    PubMed

    Clark, Kevin D; Emaus, Miranda N; Varona, Marcelino; Bowers, Ashley N; Anderson, Jared L

    2018-01-01

    The applications of ionic liquids (ILs) and IL-derived sorbents are rapidly expanding. By careful selection of the cation and anion components, the physicochemical properties of ILs can be altered to meet the requirements of specific applications. Reports of IL solvents possessing high selectivity for specific analytes are numerous and continue to motivate the development of new IL-based sample preparation methods that are faster, more selective, and environmentally benign compared to conventional organic solvents. The advantages of ILs have also been exploited in solid/polymer formats in which ordinarily nonspecific sorbents are functionalized with IL moieties in order to impart selectivity for an analyte or analyte class. Furthermore, new ILs that incorporate a paramagnetic component into the IL structure, known as magnetic ionic liquids (MILs), have emerged as useful solvents for bioanalytical applications. In this rapidly changing field, this Review focuses on the applications of ILs and IL-based sorbents in sample preparation with a special emphasis on liquid phase extraction techniques using ILs and MILs, IL-based solid-phase extraction, ILs in mass spectrometry, and biological applications. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. 14 CFR 121.909 - Approval of Advanced Qualification Program.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... methodology must incorporate a thorough analysis of the certificate holder's operations, aircraft, line environment and job functions. All AQP qualification and continuing qualification curriculums must integrate.... (ii) Initial job task listing. (iii) Instructional systems development methodology. (iv) Qualification...

  2. Humidity-controlled preparation of frozen-hydrated biological samples for cryogenic coherent x-ray diffraction microscopy

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Takayama, Yuki; Nakasako, Masayoshi; RIKEN Harima Institute/SPring-8, 1-1-1 Kouto, Mikaduki, Sayo, Hyogo 679-5148

    2012-05-15

    Coherent x-ray diffraction microscopy (CXDM) has the potential to visualize the structures of micro- to sub-micrometer-sized biological particles, such as cells and organelles, at high resolution. Toward advancing structural studies on the functional states of such particles, here, we developed a system for the preparation of frozen-hydrated biological samples for cryogenic CXDM experiments. The system, which comprised a moist air generator, microscope, micro-injector mounted on a micromanipulator, custom-made sample preparation chamber, and flash-cooling device, allowed for the manipulation of sample particles in the relative humidity range of 20%-94%rh at 293 K to maintain their hydrated and functional states. Here, wemore » report the details of the system and the operation procedure, including its application to the preparation of a frozen-hydrated chloroplast sample. Sample quality was evaluated through a cryogenic CXDM experiment conducted at BL29XUL of SPring-8. Taking the performance of the system and the quality of the sample, the system was suitable to prepare frozen-hydrated biological samples for cryogenic CXDM experiments.« less

  3. Implementation plans for the Korean certified tumor registrar qualification system.

    PubMed

    Boo, Yoo-Kyung; Lim, Hyun-Sook; Won, Young-Joo

    2014-01-01

    Cancer registration data is used to understand the nation's cancer burden, and to provide significant baseline data for cancer control efforts, as well as, research on cancer incidence, mortality, survival, and prevalence. A system that approves, assesses, and manages the qualification of specialists, responsible for performing cancer registration, has not been developed in Korea. This study presents ways to implement a certification system designed for the qualification of tumor registrars in Korea. Requirements for implementing a certified tumor registrar qualification system were determined by reviewing the system for establishing qualifications in Korea and the American qualification system via the National Cancer Registrars Association (NCRA). Moreover, a survey was conducted on Korean medical records administrators, who had taken the U.S. Certified Tumor Registrar (CTR) examination, in order to review their opinions regarding these requirements. This study verified the feasibility of a qualification examination based on the opinions of CTR specialists by determining the following: items, and the associated ratings, of the qualifications necessary to register individuals as certified tumor registrars in a private qualification system; status of human resources required for the examination or training processes; plans regarding the organization needed for management, and operation of qualifications, examination standards, subject areas, examination methods, examination qualifications, or education and training programs. The implementation of a certified tumor registrar qualification system will lead to enhanced job competency for specialists and a qualitative improvement of cancer registration data. It will also reliably foster human resources that will lay the groundwork needed to establish scientific and reasonable national cancer management policies.

  4. WTP Waste Feed Qualification: Hydrogen Generation Rate Measurement Apparatus Testing Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stone, M. E.; Newell, J. D.; Smith, T. E.

    The generation rate of hydrogen gas in the Hanford tank waste will be measured during the qualification of the staged tank waste for processing in the Hanford Tank Waste Treatment and Immobilization Plant. Based on a review of past practices in measurement of the hydrogen generation, an apparatus to perform this measurement has been designed and tested for use during waste feed qualification. The hydrogen generation rate measurement apparatus (HGRMA) described in this document utilized a 100 milliliter sample in a continuously-purged, continuously-stirred vessel, with measurement of hydrogen concentration in the vent gas. The vessel and lid had a combinedmore » 220 milliliters of headspace. The vent gas system included a small condenser to prevent excessive evaporative losses from the sample during the test, as well as a demister and filter to prevent particle migration from the sample to the gas chromatography system. The gas chromatograph was an on line automated instrument with a large-volume sample-injection system to allow measurement of very low hydrogen concentrations. This instrument automatically sampled the vent gas from the hydrogen generation rate measurement apparatus every five minutes and performed data regression in real time. The fabrication of the hydrogen generation rate measurement apparatus was in accordance with twenty three (23) design requirements documented in the conceptual design package, as well as seven (7) required developmental activities documented in the task plan associated with this work scope. The HGRMA was initially tested for proof of concept with physical simulants, and a remote demonstration of the system was performed in the Savannah River National Laboratory Shielded Cells Mockup Facility. Final verification testing was performed using non-radioactive simulants of the Hanford tank waste. Three different simulants were tested to bound the expected rheological properties expected during waste feed qualification testing

  5. Electrodeposition as an alternate method for preparation of environmental samples for iodide by AMS

    DOE PAGES

    Adamic, M. L.; Lister, T. E.; Dufek, E. J.; ...

    2015-03-25

    This paper presents an evaluation of an alternate method for preparing environmental samples for 129I analysis by accelerator mass spectrometry (AMS) at Idaho National Laboratory. The optimal sample preparation method is characterized by ease of preparation, capability of processing very small quantities of iodide, and ease of loading into a cathode. Electrodeposition of iodide on a silver wire was evaluated using these criteria. This study indicates that the electrochemically-formed silver iodide deposits produce ion currents similar to those from precipitated silver iodide for the same sample mass. Furthermore, precipitated silver iodide samples are usually mixed with niobium or silver powdermore » prior to loading in a cathode. Using electrodeposition, the silver is already mixed with the sample and can simply be picked up with tweezers, placed in the sample die, and pressed into a cathode. The major advantage of this method is that the silver wire/electrodeposited silver iodide is much easier to load into a cathode.« less

  6. The "Qualifications Business" in Higher Education

    ERIC Educational Resources Information Center

    Berkhout, S. J.

    2006-01-01

    Qualifications have become academic currency. Apart from adding to the success and fluency with which people can move or be transferred from job to job, nationally and internationally, qualifications also serve to shape individuals' perceptions of their own worth because of their impact on their holders' expectations and prospects, as well as…

  7. Response Variability in Commercial MOSFET SEE Qualification

    DOE PAGES

    George, J. S.; Clymer, D. A.; Turflinger, T. L.; ...

    2016-12-01

    Single-event effects (SEE) evaluation of five different part types of next generation, commercial trench MOSFETs indicates large part-to-part variation in determining a safe operating area (SOA) for drain-source voltage (V DS) following a test campaign that exposed >50 samples per part type to heavy ions. These results suggest a determination of a SOA using small sample sizes may fail to capture the full extent of the part-to-part variability. An example method is discussed for establishing a Safe Operating Area using a one-sided statistical tolerance limit based on the number of test samples. Finally, burn-in is shown to be a criticalmore » factor in reducing part-to-part variation in part response. Implications for radiation qualification requirements are also explored.« less

  8. Response Variability in Commercial MOSFET SEE Qualification

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    George, J. S.; Clymer, D. A.; Turflinger, T. L.

    Single-event effects (SEE) evaluation of five different part types of next generation, commercial trench MOSFETs indicates large part-to-part variation in determining a safe operating area (SOA) for drain-source voltage (V DS) following a test campaign that exposed >50 samples per part type to heavy ions. These results suggest a determination of a SOA using small sample sizes may fail to capture the full extent of the part-to-part variability. An example method is discussed for establishing a Safe Operating Area using a one-sided statistical tolerance limit based on the number of test samples. Finally, burn-in is shown to be a criticalmore » factor in reducing part-to-part variation in part response. Implications for radiation qualification requirements are also explored.« less

  9. Compact low temperature scanning tunneling microscope with in-situ sample preparation capability

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kim, Jungdae; Department of Physics and EHSRC, University of Ulsan, Ulsan 680-749; Nam, Hyoungdo

    2015-09-15

    We report on the design of a compact low temperature scanning tunneling microscope (STM) having in-situ sample preparation capability. The in-situ sample preparation chamber was designed to be compact allowing quick transfer of samples to the STM stage, which is ideal for preparing temperature sensitive samples such as ultra-thin metal films on semiconductor substrates. Conventional spring suspensions on the STM head often cause mechanical issues. To address this problem, we developed a simple vibration damper consisting of welded metal bellows and rubber pads. In addition, we developed a novel technique to ensure an ultra-high-vacuum (UHV) seal between the copper andmore » stainless steel, which provides excellent reliability for cryostats operating in UHV. The performance of the STM was tested from 2 K to 77 K by using epitaxial thin Pb films on Si. Very high mechanical stability was achieved with clear atomic resolution even when using cryostats operating at 77 K. At 2 K, a clean superconducting gap was observed, and the spectrum was easily fit using the BCS density of states with negligible broadening.« less

  10. Improved sample preparation and counting techniques for enhanced tritium measurement sensitivity

    NASA Astrophysics Data System (ADS)

    Moran, J.; Aalseth, C.; Bailey, V. L.; Mace, E. K.; Overman, C.; Seifert, A.; Wilcox Freeburg, E. D.

    2015-12-01

    Tritium (T) measurements offer insight to a wealth of environmental applications including hydrologic tracking, discerning ocean circulation patterns, and aging ice formations. However, the relatively short half-life of T (12.3 years) limits its effective age dating range. Compounding this limitation is the decrease in atmospheric T content by over two orders of magnitude (from 1000-2000 TU in 1962 to < 10 TU currently) since the cessation of above ground nuclear testing in the 1960's. We are developing sample preparation methods coupled to direct counting of T via ultra-low background proportional counters which, when combined, offer improved T measurement sensitivity (~4.5 mmoles of H2 equivalent) and will help expand the application of T age dating to smaller sample sizes linked to persistent environmental questions despite the limitations above. For instance, this approach can be used to T date ~ 2.2 mmoles of CH4 collected from sample-limited systems including microbial communities, soils, or subsurface aquifers and can be combined with radiocarbon dating to distinguish the methane's formation age from C age in a system. This approach can also expand investigations into soil organic C where the improved sensitivity will permit resolution of soil C into more descriptive fractions and provide direct assessments of the stability of specific classes of organic matter in soils environments. We are employing a multiple step sample preparation system whereby organic samples are first combusted with resulting CO2 and H2O being used as a feedstock to synthesize CH4. This CH4 is mixed with Ar and loaded directly into an ultra-low background proportional counter for measurement of T β decay in a shallow underground laboratory. Analysis of water samples requires only the addition of geologic CO2 feedstock with the sample for methane synthesis. The chemical nature of the preparation techniques enable high sample throughput with only the final measurement requiring T decay

  11. Sample preparation for thermo-gravimetric determination and thermo-gravimetric characterization of refuse derived fuel.

    PubMed

    Robinson, T; Bronson, B; Gogolek, P; Mehrani, P

    2016-02-01

    Thermo-gravimetric analysis (TGA) is a useful method for characterizing fuels. In the past it has been applied to the study of refuse derived fuel (RDF) and related materials. However, the heterogeneity of RDF makes the preparation of small representative samples very difficult and this difficulty has limited the effectiveness of TGA for characterization of RDF. A TGA method was applied to a variety of materials prepared from a commercially available RDF using a variety of procedures. Applicability of TGA method to the determination of the renewable content of RDF was considered. Cryogenic ball milling was found to be an effective means of preparing RDF samples for TGA. When combined with an effective sample preparation, TGA could be used as an alternative method for assessing the renewable content of RDF. Crown Copyright © 2015. Published by Elsevier Ltd. All rights reserved.

  12. Inspection qualification (IQ) and operational qualification (OQ) for a vacuum freeze-dryer--Part I. General discussion.

    PubMed

    Jennings, T A; Scheer, A; Emodi, A; Puderbach, L; King, S; Norton, T

    1996-01-01

    The principle objectives of this paper are (a), to develop the rationale for conducting an inspection qualification (IQ) and operational qualification (OQ) of a vacuum freeze-dryer; (b), to identify the key elements that require verification for completion of the IQ; and (c), to establish the necessary environmental and operational parameters necessary for the OQ of the vacuum freeze-dryer.

  13. Sample preparation for the analysis of isoflavones from soybeans and soy foods.

    PubMed

    Rostagno, M A; Villares, A; Guillamón, E; García-Lafuente, A; Martínez, J A

    2009-01-02

    This manuscript provides a review of the actual state and the most recent advances as well as current trends and future prospects in sample preparation and analysis for the quantification of isoflavones from soybeans and soy foods. Individual steps of the procedures used in sample preparation, including sample conservation, extraction techniques and methods, and post-extraction treatment procedures are discussed. The most commonly used methods for extraction of isoflavones with both conventional and "modern" techniques are examined in detail. These modern techniques include ultrasound-assisted extraction, pressurized liquid extraction, supercritical fluid extraction and microwave-assisted extraction. Other aspects such as stability during extraction and analysis by high performance liquid chromatography are also covered.

  14. An Effective Preparation for Tertiary Mathematics

    ERIC Educational Resources Information Center

    Parnell, Sheena; Statham, Moira

    2007-01-01

    The University of Auckland offers a one-year pre-degree Tertiary Foundation Certificate (TFC) Programme, for up to 200 students who are under-prepared for university study. The students may come straight from school without having gained a university entrance qualification, or they may be students returning to education who lack the confidence to…

  15. 47 CFR 54.415 - Consumer qualification for Link Up.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 3 2010-10-01 2010-10-01 false Consumer qualification for Link Up. 54.415... (CONTINUED) UNIVERSAL SERVICE Universal Service Support for Low-Income Consumers § 54.415 Consumer qualification for Link Up. (a) In a state that mandates state Lifeline support, the consumer qualification...

  16. 46 CFR 57.05-2 - Transfer of performance qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Transfer of performance qualifications. 57.05-2 Section 57.05-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-2 Transfer of performance qualifications. (a) The...

  17. 46 CFR 57.05-2 - Transfer of performance qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 2 2012-10-01 2012-10-01 false Transfer of performance qualifications. 57.05-2 Section 57.05-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-2 Transfer of performance qualifications. (a) The...

  18. 46 CFR 57.05-2 - Transfer of performance qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 2 2013-10-01 2013-10-01 false Transfer of performance qualifications. 57.05-2 Section 57.05-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-2 Transfer of performance qualifications. (a) The...

  19. 46 CFR 57.05-2 - Transfer of performance qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 2 2011-10-01 2011-10-01 false Transfer of performance qualifications. 57.05-2 Section 57.05-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-2 Transfer of performance qualifications. (a) The...

  20. 46 CFR 57.05-2 - Transfer of performance qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 2 2014-10-01 2014-10-01 false Transfer of performance qualifications. 57.05-2 Section 57.05-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-2 Transfer of performance qualifications. (a) The...

  1. 47 CFR 54.415 - Consumer qualification for Link Up.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 3 2011-10-01 2011-10-01 false Consumer qualification for Link Up. 54.415... (CONTINUED) UNIVERSAL SERVICE Universal Service Support for Low-Income Consumers § 54.415 Consumer qualification for Link Up. (a) In a state that mandates state Lifeline support, the consumer qualification...

  2. 40 CFR 205.171-2 - Test exhaust system sample selection and preparation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Test exhaust system sample selection... Systems § 205.171-2 Test exhaust system sample selection and preparation. (a)(1) Exhaust systems comprising the sample which are required to be tested under a test request in accordance with this subpart...

  3. Kentucky Teacher Preparation and Certification Handbook. 1983 Edition.

    ERIC Educational Resources Information Center

    Kentucky State Dept. of Education, Frankfort. Div. of Teacher Education and Certification.

    This handbook includes teacher preparation and certification regulations adopted by the Kentucky State Board of Education through March 1983. State standards are delineated for: (1) statutory provisions relating to teacher education and certification; (2) teacher certification procedures; (3) ranking of teacher qualifications; (4) evaluation and…

  4. TruSeq Stranded mRNA and Total RNA Sample Preparation Kits

    Cancer.gov

    Total RNA-Seq enabled by ribosomal RNA (rRNA) reduction is compatible with formalin-fixed paraffin embedded (FFPE) samples, which contain potentially critical biological information. The family of TruSeq Stranded Total RNA sample preparation kits provides a unique combination of unmatched data quality for both mRNA and whole-transcriptome analyses, robust interrogation of both standard and low-quality samples and workflows compatible with a wide range of study designs.

  5. Evaporator Feed Qualification Analysis Of Tank 38H And 43H Samples: January 2010 Through April 2013

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Martino, C. J.; Coleman, C. J.

    2013-08-21

    This report provides the results of analyses that focused on the chemical species that pertain to the sodium aluminosilicate formation potential for archived Tank 38H and 43H subsurface samples from January 2010 through April 2013. Analyses included warm acid strike preparation followed by analysis of silicon, aluminum, and sodium and water dilution preparation followed by analysis for anions. The Tank 43H and 38H supernatant liquid silicon measurements for the January 2010 through April 2013 time period exhibit a slight increasing trend. Over this time period, the silicon concentration in the Tank 43H and Tank 38H samples averaged 179 mg/L andmore » 235 mg/L, respectively. Comparison of Tank 43H sample results from 2005 through April 2013 to the previously developed process control models indicates that the current formation of sodium aluminosilicate in the 2H system is due to the seeded direct precipitation of cancrinite and sodalite.« less

  6. On the Functions of a System of Vocational Qualifications

    ERIC Educational Resources Information Center

    Lum, Gerard

    2015-01-01

    While acknowledging that a system of vocational qualifications might be perceived as having any number of possible purposes, this paper identifies three primary functions that any vocational qualification must fulfil by dint of being a vocational qualification. It is argued that current arrangements are unable to fulfil these essential functions…

  7. A Combined Fabrication and Instrumentation Platform for Sample Preparation.

    PubMed

    Guckenberger, David J; Thomas, Peter C; Rothbauer, Jacob; LaVanway, Alex J; Anderson, Meghan; Gilson, Dan; Fawcett, Kevin; Berto, Tristan; Barrett, Kevin; Beebe, David J; Berry, Scott M

    2014-06-01

    While potentially powerful, access to molecular diagnostics is substantially limited in the developing world. Here we present an approach to reduced cost molecular diagnostic instrumentation that has the potential to empower developing world communities by reducing costs through streamlining the sample preparation process. In addition, this instrument is capable of producing its own consumable devices on demand, reducing reliance on assay suppliers. Furthermore, this instrument is designed with an "open" architecture, allowing users to visually observe the assay process and make modifications as necessary (as opposed to traditional "black box" systems). This open environment enables integration of microfluidic fabrication and viral RNA purification onto an easy-to-use modular system via the use of interchangeable trays. Here we employ this system to develop a protocol to fabricate microfluidic devices and then use these devices to isolate viral RNA from serum for the measurement of human immunodeficiency virus (HIV) viral load. Results obtained from this method show significantly reduced error compared with similar nonautomated sample preparation processes. © 2014 Society for Laboratory Automation and Screening.

  8. Organizational characteristics influencing nursing home social service directors' qualifications: a national study.

    PubMed

    Simons, Kelsey V

    2006-11-01

    This research sought to identify organizational characteristics associated with the amount of professional qualifications among a nationally representative sample of nursing home social service directors. A self-administered survey was sent to directors in 675 facilities randomly sampled from a federal database, excluding facilities with fewer than 120 beds that are not required to staff a full-time social worker. The response rate was 45 percent (N = 299). Univariate results showed that most respondents possessed a social work degree, most lacked licensure, and few were clinically supervised. A multiple regression analysis found that nonprofit, independently owned facilities in rural areas staffed social service directors who were significantly more qualified than directors in for-profit, chain-affiliated facilities in urban and suburban areas. Facilities with fewer psychosocial deficiencies and higher occupancy rates employed social service directors with greater qualifications. The implications of these findings for social work education, practice, policy, and research are discussed.

  9. Basic tree-ring sample preparation techniques for aging aspen

    Treesearch

    Lance A. Asherin; Stephen A. Mata

    2001-01-01

    Aspen is notoriously difficult to age because of its light-colored wood and faint annual growth rings. Careful preparation and processing of aspen ring samples can overcome these problems, yield accurate age and growth estimates, and concisely date disturbance events present in the tree-ring record. Proper collection of aspen wood is essential in obtaining usable ring...

  10. Building the Qualification File of EGNOS with DOORS

    NASA Astrophysics Data System (ADS)

    Fabre, J.

    2008-08-01

    EGNOS, the European Satellite-Based Augmentation System (SBAS) to GPS, is getting to its final deployment and being initially operated towards qualification and certification to reach operational capability by 2008/2009. A very important milestone in the development process is the System Qualification Review (QR). As the verification phase aims at demonstrating that the EGNOS System design meets the applicable requirements, the QR declares the completion of verification activities. The main document to present at QR is a consolidated, consistent and complete Qualification file. The information included shall give confidence to the QR reviewers that the performed qualification activities are completed. Therefore, an important issue for the project team is to focus on synthetic and consistent information, and to make the presentation as clear as possible. Traceability to applicable requirements shall be systematically presented. Moreover, in order to support verification justification, reference to details shall be available, and the reviewer shall have the possibility to link automatically to the documents including this detailed information. In that frame, Thales Alenia Space has implemented a strong support in terms of methodology and tool, to provide to System Engineering and Verification teams a single reference technical database, in which all team members consult the applicable requirements, compliance, justification, design data and record the information necessary to build the final Qualification file. This paper presents the EGNOS context, the Qualification file contents, and the methodology implemented, based on Thales Alenia Space practices and in line with ECSS. Finally, it shows how the Qualification file is built in a DOORS environment.

  11. Preparation of pure microbiological samples for pyrolysis gas-liquid chromatography studies

    NASA Technical Reports Server (NTRS)

    Oxborrow, G. S.; Fields, N. D.; Puleo, J. R.

    1976-01-01

    Bacterial samples were prepared for pyrolysis gas-liquid chromatography using cells grown on membrane filters. Pyrochromatograms were reproducible when cells harvested from the filters were pyrolyzed without being washed.

  12. Microfluidic devices for sample preparation and rapid detection of foodborne pathogens.

    PubMed

    Kant, Krishna; Shahbazi, Mohammad-Ali; Dave, Vivek Priy; Ngo, Tien Anh; Chidambara, Vinayaka Aaydha; Than, Linh Quyen; Bang, Dang Duong; Wolff, Anders

    2018-03-10

    Rapid detection of foodborne pathogens at an early stage is imperative for preventing the outbreak of foodborne diseases, known as serious threats to human health. Conventional bacterial culturing methods for foodborne pathogen detection are time consuming, laborious, and with poor pathogen diagnosis competences. This has prompted researchers to call the current status of detection approaches into question and leverage new technologies for superior pathogen sensing outcomes. Novel strategies mainly rely on incorporating all the steps from sample preparation to detection in miniaturized devices for online monitoring of pathogens with high accuracy and sensitivity in a time-saving and cost effective manner. Lab on chip is a blooming area in diagnosis, which exploits different mechanical and biological techniques to detect very low concentrations of pathogens in food samples. This is achieved through streamlining the sample handling and concentrating procedures, which will subsequently reduce human errors and enhance the accuracy of the sensing methods. Integration of sample preparation techniques into these devices can effectively minimize the impact of complex food matrix on pathogen diagnosis and improve the limit of detections. Integration of pathogen capturing bio-receptors on microfluidic devices is a crucial step, which can facilitate recognition abilities in harsh chemical and physical conditions, offering a great commercial benefit to the food-manufacturing sector. This article reviews recent advances in current state-of-the-art of sample preparation and concentration from food matrices with focus on bacterial capturing methods and sensing technologies, along with their advantages and limitations when integrated into microfluidic devices for online rapid detection of pathogens in foods and food production line. Copyright © 2018. Published by Elsevier Inc.

  13. 14 CFR 60.15 - Initial qualification requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Initial qualification requirements. 60.15 Section 60.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.15...

  14. 14 CFR 60.15 - Initial qualification requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Initial qualification requirements. 60.15 Section 60.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.15...

  15. 14 CFR 60.7 - Sponsor qualification requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Sponsor qualification requirements. 60.7 Section 60.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.7...

  16. 14 CFR 60.7 - Sponsor qualification requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Sponsor qualification requirements. 60.7 Section 60.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.7...

  17. 14 CFR 60.7 - Sponsor qualification requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Sponsor qualification requirements. 60.7 Section 60.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.7...

  18. 14 CFR 60.15 - Initial qualification requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Initial qualification requirements. 60.15 Section 60.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.15...

  19. 14 CFR 60.7 - Sponsor qualification requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Sponsor qualification requirements. 60.7 Section 60.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.7...

  20. 14 CFR 60.15 - Initial qualification requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Initial qualification requirements. 60.15 Section 60.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.15...

  1. 14 CFR 60.4 - Qualification Performance Standards.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Qualification Performance Standards. 60.4 Section 60.4 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.4...

  2. 14 CFR 60.4 - Qualification Performance Standards.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Qualification Performance Standards. 60.4 Section 60.4 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.4...

  3. 14 CFR 60.15 - Initial qualification requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Initial qualification requirements. 60.15 Section 60.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.15...

  4. 14 CFR 60.4 - Qualification Performance Standards.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Qualification Performance Standards. 60.4 Section 60.4 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.4...

  5. 14 CFR 60.4 - Qualification Performance Standards.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Qualification Performance Standards. 60.4 Section 60.4 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.4...

  6. 14 CFR 60.4 - Qualification Performance Standards.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Qualification Performance Standards. 60.4 Section 60.4 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN FLIGHT SIMULATION TRAINING DEVICE INITIAL AND CONTINUING QUALIFICATION AND USE § 60.4...

  7. 48 CFR 1437.7001 - Contractor qualification requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contractor qualification requirements. 1437.7001 Section 1437.7001 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR... Contractor qualification requirements. (a) Prior to award of a contract for real property appraisal services...

  8. Protocols for the analytical characterization of therapeutic monoclonal antibodies. II - Enzymatic and chemical sample preparation.

    PubMed

    Bobaly, Balazs; D'Atri, Valentina; Goyon, Alexandre; Colas, Olivier; Beck, Alain; Fekete, Szabolcs; Guillarme, Davy

    2017-08-15

    The analytical characterization of therapeutic monoclonal antibodies and related proteins usually incorporates various sample preparation methodologies. Indeed, quantitative and qualitative information can be enhanced by simplifying the sample, thanks to the removal of sources of heterogeneity (e.g. N-glycans) and/or by decreasing the molecular size of the tested protein by enzymatic or chemical fragmentation. These approaches make the sample more suitable for chromatographic and mass spectrometric analysis. Structural elucidation and quality control (QC) analysis of biopharmaceutics are usually performed at intact, subunit and peptide levels. In this paper, general sample preparation approaches used to attain peptide, subunit and glycan level analysis are overviewed. Protocols are described to perform tryptic proteolysis, IdeS and papain digestion, reduction as well as deglycosylation by PNGase F and EndoS2 enzymes. Both historical and modern sample preparation methods were compared and evaluated using rituximab and trastuzumab, two reference therapeutic mAb products approved by Food and Drug Administration (FDA) and European Medicines Agency (EMA). The described protocols may help analysts to develop sample preparation methods in the field of therapeutic protein analysis. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. The NOSAMS sample preparation laboratory in the next millenium: Progress after the WOCE program

    NASA Astrophysics Data System (ADS)

    Gagnon, Alan R.; McNichol, Ann P.; Donoghue, Joanne C.; Stuart, Dana R.; von Reden, Karl; Nosams

    2000-10-01

    Since 1991, the primary charge of the National Ocean Sciences AMS (NOSAMS) facility at the Woods Hole Oceanographic Institution has been to supply high throughput, high precision AMS 14C analyses for seawater samples collected as part of the World Ocean Circulation Experiment (WOCE). Approximately 13,000 samples taken as part of WOCE should be fully analyzed by the end of Y2K. Additional sample sources and techniques must be identified and incorporated if NOSAMS is to continue in its present operation mode. A trend in AMS today is the ability to routinely process and analyze radiocarbon samples that contain tiny amounts (<100 μg) of carbon. The capability to mass-produce small samples for 14C analysis has been recognized as a major facility goal. The installation of a new 134-position MC-SNICS ion source, which utilizes a smaller graphite target cartridge than presently used, is one step towards realizing this goal. New preparation systems constructed in the sample preparation laboratory (SPL) include an automated bank of 10 small-volume graphite reactors, an automated system to process organic carbon samples, and a multi-dimensional preparative capillary gas chromatograph (PCGC).

  10. [Standard sample preparation method for quick determination of trace elements in plastic].

    PubMed

    Yao, Wen-Qing; Zong, Rui-Long; Zhu, Yong-Fa

    2011-08-01

    Reference sample was prepared by masterbatch method, containing heavy metals with known concentration of electronic information products (plastic), the repeatability and precision were determined, and reference sample preparation procedures were established. X-Ray fluorescence spectroscopy (XRF) analysis method was used to determine the repeatability and uncertainty in the analysis of the sample of heavy metals and bromine element. The working curve and the metrical methods for the reference sample were carried out. The results showed that the use of the method in the 200-2000 mg x kg(-1) concentration range for Hg, Pb, Cr and Br elements, and in the 20-200 mg x kg(-1) range for Cd elements, exhibited a very good linear relationship, and the repeatability of analysis methods for six times is good. In testing the circuit board ICB288G and ICB288 from the Mitsubishi Heavy Industry Company, results agreed with the recommended values.

  11. Professional Qualifications of Teachers for English for Primary and Secondary Education--A Brief Comparison between Hong Kong and China

    ERIC Educational Resources Information Center

    Mak, Barley

    2016-01-01

    Entry to the teaching career in most education systems nowadays is based on academic qualifications and professional credentials. The requirements and trainings for teachers of English vary in different regions and countries. This short review presents the professional preparation of pre-service teachers of English and the English language…

  12. Cytotoxicity of Light-Cured Dental Materials according to Different Sample Preparation Methods

    PubMed Central

    Lee, Myung-Jin; Kim, Mi-Joo; Kwon, Jae-Sung; Lee, Sang-Bae; Kim, Kwang-Mahn

    2017-01-01

    Dental light-cured resins can undergo different degrees of polymerization when applied in vivo. When polymerization is incomplete, toxic monomers may be released into the oral cavity. The present study assessed the cytotoxicity of different materials, using sample preparation methods that mirror clinical conditions. Composite and bonding resins were used and divided into four groups according to sample preparation method: uncured; directly cured samples, which were cured after being placed on solidified agar; post-cured samples were polymerized before being placed on agar; and “removed unreacted layer” samples had their oxygen-inhibition layer removed after polymerization. Cytotoxicity was evaluated using an agar diffusion test, MTT assay, and confocal microscopy. Uncured samples were the most cytotoxic, while removed unreacted layer samples were the least cytotoxic (p < 0.05). In the MTT assay, cell viability increased significantly in every group as the concentration of the extracts decreased (p < 0.05). Extracts from post-cured and removed unreacted layer samples of bonding resin were less toxic than post-cured and removed unreacted layer samples of composite resin. Removal of the oxygen-inhibition layer resulted in the lowest cytotoxicity. Clinicians should remove unreacted monomers on the resin surface immediately after restoring teeth with light-curing resin to improve the restoration biocompatibility. PMID:28772647

  13. The Phobos-Grunt microgravity soil preparation system

    NASA Astrophysics Data System (ADS)

    Yung, Kai-leung; Lam, Chi Wo; Ko, Sui Man; Foster, James Abbott

    2017-12-01

    To understand the composition of regolith on distant bodies it is important to make quantitative measurement of its composition. However, many instruments on board space missions can only make qualitative measurements. The SOil Preparation SYStem (SOPSYS) designed for the Phobos-grunt mission in 2011 was a unique spacecraft subsystem that can grind, sieve, transport and measure samples of regolith in the absence of gravity. Its mission was to produce a compact plug of regolith sample composed of particles no larger than 1 mm for a gas analytic package. It delivers a sample with specified volume enabling a quantitative analysis of the volatiles produced at different temperatures through heating. To minimize cross contamination, SOPSYS self-cleans after each sample is delivered. The apparatus was a cooperative development between China and Russia for the Phobos-Grunt mission to the Martian moon Phobos and will be reused on the upcoming reattempt of that mission and other similar missions. The paper presents an overview of the subsystem and the results of qualification model testing. The flight unit of SOPSYS has a low mass of 622 g including control electronics and compact dimensions of 247 mm by 102 mm by 45 mm.

  14. 16 CFR § 1210.14 - Qualification testing.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Qualification testing. § 1210.14 Section § 1210.14 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS SAFETY STANDARD FOR CIGARETTE LIGHTERS Certification Requirements § 1210.14 Qualification testing. (a...

  15. 48 CFR 34.103 - Testing and qualification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Developed Under Title III, Defense Production Act 34.103 Testing and qualification. (a) Contractors... or item of supply; and (2) for major systems in production, remaining quantities to be acquired are sufficient to justify incurring the cost of testing and qualification. In evaluating this request, the...

  16. Sample preparation prior to the LC-MS-based metabolomics/metabonomics of blood-derived samples.

    PubMed

    Gika, Helen; Theodoridis, Georgios

    2011-07-01

    Blood represents a very important biological fluid and has been the target of continuous and extensive research for diagnostic, or health and drug monitoring reasons. Recently, metabonomics/metabolomics have emerged as a new and promising 'omics' platform that shows potential in biomarker discovery, especially in areas such as disease diagnosis, assessment of drug efficacy or toxicity. Blood is collected in various establishments in conditions that are not standardized. Next, the samples are prepared and analyzed using different methodologies or tools. When targeted analysis of key molecules (e.g., a drug or its metabolite[s]) is the aim, enforcement of certain measures or additional analyses may correct and harmonize these discrepancies. In omics fields such as those performed by holistic analytical approaches, no such rules or tools are available. As a result, comparison or correlation of results or data fusion becomes impractical. However, it becomes evident that such obstacles should be overcome in the near future to allow for large-scale studies that involve the assaying of samples from hundreds of individuals. In this case the effect of sample handling and preparation becomes very serious, in order to avoid wasting months of work from experts and expensive instrument time. The present review aims to cover the different methodologies applied to the pretreatment of blood prior to LC-MS metabolomic/metabonomic studies. The article tries to critically compare the methods and highlight issues that need to be addressed.

  17. Investigating a Drop-on-Demand Microdispenser for Standardized Sample Preparation

    DTIC Science & Technology

    2012-09-01

    Demand Microdispenser for Standardized Sample Preparation Ellen L. Holthoff, Mikella E. Farrell, and Paul M. Pellegrino Sensors and Electron Devices...instrument. The use of a gravimetric method, such as a quartz crystal microbalance4 ( QCM ) or a sensitive microbalance as discussed in this report, is

  18. Preparation of graphene thin films for radioactive samples.

    PubMed

    Roteta, Miguel; Fernández-Martínez, Rodolfo; Mejuto, Marcos; Rucandio, Isabel

    2016-03-01

    A new method for the preparation of conductive thin films is presented. The metallization of VYNS films guarantees the electrical conductivity but it results in the breaking of a high proportion of them. Graphene, a two-dimensional nanostructure of monolayer or few layers graphite has attracted a great deal of attention because of its excellent properties such as a good chemical stability, mechanical resistance and extraordinary electronic transport properties. In this work, the possibilities of graphene have been explored as a way to produce electrical conductive thin films without an extra metallization process. The procedure starts with preparing homogenous suspensions of reduced graphene oxide (rGO) in conventional VYNS solutions. Ultra-sonication is used to ensure a good dispersibility of rGO. Graphene oxide (GO) is prepared via oxidation of graphite and subsequent exfoliation by sonication. Different chemically rGO were obtained by reaction with hydrazine sulfate, sodium borohydride, ascorbic acid and hydroiodic acid as reducing agents. The preparation of the thin graphene films is done in a similar way as the conventional VYNS foil preparation procedure. Drops of the solution are deposited onto water. The graphene films have been used to prepare sources containing some electron capture radionuclides ((109)Cd, (55)Fe, (139)Ce) with an activity in the order of 3kBq. The samples have been measured to test the attainable low energy electron efficiency and the energy resolution of Auger and conversion electrons by 4π (electron capture)-γ coincidence measurements. The 4π (electron capture)-γ coincidence setup includes a pressurized proportional counter and a NaI(Tl) detector. Tests with different pressures up to 1000kPa were carried out. All these tests show similar values in both parameters (efficiency and resolution) as those obtained by using the conventional metallized films without the drawback of the high percentage of broken films. Copyright © 2015

  19. Impact of the Europeanisation of Education: Qualifications Frameworks in Europe

    ERIC Educational Resources Information Center

    Mikulec, Borut

    2017-01-01

    This article examines the influence of the European qualifications framework--a key European lifelong learning policy instrument for improving employability, comparability and mobility in the European educational space--on the establishment of national qualifications frameworks in Europe. The European qualifications framework and national…

  20. 49 CFR 213.345 - Vehicle qualification testing.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 4 2012-10-01 2012-10-01 false Vehicle qualification testing. 213.345 Section 213... Higher § 213.345 Vehicle qualification testing. (a) All rolling stock types which operate at Class 6... demonstrate that the vehicle dynamic response to track alinement and geometry variations are within acceptable...

  1. 30 CFR 281.4 - Qualifications of lessees.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Qualifications of lessees. 281.4 Section 281.4 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR OFFSHORE LEASING OF MINERALS OTHER THAN OIL, GAS, AND SULPHUR IN THE OUTER CONTINENTAL SHELF General § 281.4 Qualifications of...

  2. 45 CFR 1306.21 - Staff qualification requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 4 2012-10-01 2012-10-01 false Staff qualification requirements. 1306.21 Section 1306.21 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE OF HUMAN DEVELOPMENT... Requirements § 1306.21 Staff qualification requirements. Head Start programs must comply with section 648A of...

  3. 45 CFR 1306.21 - Staff qualification requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 4 2014-10-01 2014-10-01 false Staff qualification requirements. 1306.21 Section 1306.21 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE OF HUMAN DEVELOPMENT... Requirements § 1306.21 Staff qualification requirements. Head Start programs must comply with section 648A of...

  4. 45 CFR 1306.21 - Staff qualification requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Staff qualification requirements. 1306.21 Section 1306.21 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE OF HUMAN DEVELOPMENT... Requirements § 1306.21 Staff qualification requirements. Head Start programs must comply with section 648A of...

  5. 45 CFR 1306.21 - Staff qualification requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 4 2011-10-01 2011-10-01 false Staff qualification requirements. 1306.21 Section 1306.21 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE OF HUMAN DEVELOPMENT... Requirements § 1306.21 Staff qualification requirements. Head Start programs must comply with section 648A of...

  6. 45 CFR 1306.21 - Staff qualification requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 4 2013-10-01 2013-10-01 false Staff qualification requirements. 1306.21 Section 1306.21 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE OF HUMAN DEVELOPMENT... Requirements § 1306.21 Staff qualification requirements. Head Start programs must comply with section 648A of...

  7. Anechoic chamber qualification at ultrasonic frequencies

    NASA Astrophysics Data System (ADS)

    Jenny, Trevor; Anderson, Brian

    2010-10-01

    Qualifying an anechoic chamber for frequencies that extend into the ultrasonic range is necessary for research work involving airborne ultrasonic sound. For example, an anechoic chamber allows for measurements of the direct sound radiated by an object without reflections from walls. The ANSI S12.55/ISO 3745 standard which covers anechoic chamber qualification does not extend into the ultrasonic frequency range, nor have others discussed this frequency range in the literature. An increasing number of technologies are employing ultrasound; hence the need to develop facilities to conduct basic research studies on airborne ultrasound. This presentation will discuss the challenges associated with chamber qualification and present the results for qualification of a chamber at Brigham Young University. [This work has been funded by the Los Alamos National Laboratory

  8. Equality of employment opportunities for nurses at the point of qualification: an exploratory study.

    PubMed

    Harris, Ruth; Ooms, Ann; Grant, Robert; Marshall-Lucette, Sylvie; Chu, Christine Sek Fun; Sayer, Jane; Burke, Linda

    2013-03-01

    Securing employment after qualification is of utmost importance to newly qualified nurses to consolidate knowledge and skills. The factors that influence success in gaining this first post are not known. The study aimed to describe the first post gained after qualification in terms of setting, nature of employment contract and geographical distribution and explore the relationship between a range of factors (including ethnicity) and employment at the point of qualification. An exploratory study using structured questionnaires and secondary analysis of data routinely collected by the universities about students and their progress during their course. The study was conducted in eight universities within a large, multicultural city in the UK as part of the 'Readiness for Work' research programme. Eight hundred and four newly qualified nurses who had successfully completed a diploma or degree from one of the universities; a response rate of 77% representing 49% of all graduating students in the study population. Data were collected by self-completed semi-structured questionnaires administered to students at the time of qualification and at three months post-qualification. Routinely collected data from the universities were also collected. Fifty two percent of participants had been offered a job at the point of qualification (85% of those who had applied and been interviewed). Of these, 99% had been offered a nursing post, 88% in the city studied, 67% in the healthcare setting where they had completed a course placement. 44% felt "confident" and 32% "very confident" about their employment prospects. Predictors of employment success included ethnicity, specialty of nursing and university attended. Predictors of confidence and preparedness for job seeking included ethnicity, nursing specialty, gender and grade of degree. Newly qualified nurses from non-White/British ethnic groups were less likely to get a job and feel confident about and prepared for job seeking. This

  9. Hadfield prepares to insert biological samples in the MELFI-1

    NASA Image and Video Library

    2013-01-07

    View of Canadian Space Agency (CSA) Chris Hadfield,Expedition 34 Flight Engineer (FE),preparing to insert biological samples in the Minus Eighty Laboratory Freezer for International Space Station (ISS) - (MELFI-1),in the Japanese Experiment Module (JEM) Pressurized Module (JPM). Photo was taken during Expedition 34.

  10. 10 CFR 1046.11 - Medical and physical fitness qualification standards.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Medical and physical fitness qualification standards. 1046... INTERESTS Protective Force Personnel § 1046.11 Medical and physical fitness qualification standards. (a... personnel any individual who fails to meet the applicable medical and physical fitness qualification...

  11. 10 CFR 1046.11 - Medical and physical fitness qualification standards.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Medical and physical fitness qualification standards. 1046... INTERESTS Protective Force Personnel § 1046.11 Medical and physical fitness qualification standards. (a... personnel any individual who fails to meet the applicable medical and physical fitness qualification...

  12. 10 CFR 1046.11 - Medical and physical fitness qualification standards.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Medical and physical fitness qualification standards. 1046... INTERESTS Protective Force Personnel § 1046.11 Medical and physical fitness qualification standards. (a... personnel any individual who fails to meet the applicable medical and physical fitness qualification...

  13. Sample preparation: a critical step in the analysis of cholesterol oxidation products.

    PubMed

    Georgiou, Christiana A; Constantinou, Michalis S; Kapnissi-Christodoulou, Constantina P

    2014-02-15

    In recent years, cholesterol oxidation products (COPs) have drawn scientific interest, particularly due to their implications on human health. A big number of these compounds have been demonstrated to be cytotoxic, mutagenic, and carcinogenic. The main source of COPs is through diet, and particularly from the consumption of cholesterol-rich foods. This raises questions about the safety of consumers, and it suggests the necessity for the development of a sensitive and a reliable analytical method in order to identify and quantify these components in food samples. Sample preparation is a necessary step in the analysis of COPs in order to eliminate interferences and increase sensitivity. Numerous publications have, over the years, reported the use of different methods for the extraction and purification of COPs. However, no method has, so far, been established as a routine method for the analysis of COPs in foods. Therefore, it was considered important to overview different sample preparation procedures and evaluate the different preparative parameters, such as time of saponification, the type of organic solvents for fat extraction, the stationary phase in solid phase extraction, etc., according to recovery, precision and simplicity. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. The European Qualifications Framework: A Technical Critique

    ERIC Educational Resources Information Center

    Lester, Stan

    2015-01-01

    The European Qualifications Framework (EQF) was introduced in 2008 as a "meta-framework" or common reference point for national qualifications frameworks in Europe, a function for which, with some caveats, it has been pragmatically successful. It has also been used with variable success to support the development or referencing of…

  15. 30 CFR 880.11 - Qualifications of projects.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ABANDONED MINE LAND RECLAMATION MINE FIRE CONTROL § 880.11 Qualifications of projects. The purpose of all projects is to prevent injury and loss of life, protect public health, conserve natural resources, or... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Qualifications of projects. 880.11 Section 880...

  16. 46 CFR 68.37 - Cessation of qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... DOCUMENTATION OF VESSELS: EXCEPTIONS TO COASTWISE QUALIFICATION Documentation of Certain Vessels for Oil Spill Cleanup § 68.37 Cessation of qualifications. (a) If the vessel is owned by a not-for-profit oil spill... change. The not-for-profit oil spill response cooperative shall report the change in writing to the...

  17. 46 CFR 68.37 - Cessation of qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... DOCUMENTATION OF VESSELS: EXCEPTIONS TO COASTWISE QUALIFICATION Documentation of Certain Vessels for Oil Spill Cleanup § 68.37 Cessation of qualifications. (a) If the vessel is owned by a not-for-profit oil spill... change. The not-for-profit oil spill response cooperative shall report the change in writing to the...

  18. 46 CFR 68.37 - Cessation of qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... DOCUMENTATION OF VESSELS: EXCEPTIONS TO COASTWISE QUALIFICATION Documentation of Certain Vessels for Oil Spill Cleanup § 68.37 Cessation of qualifications. (a) If the vessel is owned by a not-for-profit oil spill... change. The not-for-profit oil spill response cooperative shall report the change in writing to the...

  19. Spectral reproducibility and quantification of peptides in MALDI of samples prepared by micro-spotting.

    PubMed

    Bae, Yong Jin; Park, Kyung Man; Ahn, Sung Hee; Moon, Jeong Hee; Kim, Myung Soo

    2014-08-01

    Previously, we reported that MALDI spectra of peptides became reproducible when temperature was kept constant. Linear calibration curves derived from such spectral data could be used for quantification. Homogeneity of samples was one of the requirements. Among the three popular matrices used in peptide MALDI [i.e., α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), and sinapinic acid (SA)], homogeneous samples could be prepared by conventional means only for CHCA. In this work, we showed that sample preparation by micro-spotting improved the homogeneity for all three cases.

  20. 46 CFR 57.05-4 - Welder qualification by procedure tests.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Welder qualification by procedure tests. 57.05-4 Section 57.05-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-4 Welder qualification by procedure tests...

  1. 46 CFR 57.05-4 - Welder qualification by procedure tests.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 2 2014-10-01 2014-10-01 false Welder qualification by procedure tests. 57.05-4 Section 57.05-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-4 Welder qualification by procedure tests...

  2. 46 CFR 57.05-4 - Welder qualification by procedure tests.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 2 2013-10-01 2013-10-01 false Welder qualification by procedure tests. 57.05-4 Section 57.05-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-4 Welder qualification by procedure tests...

  3. 46 CFR 57.05-4 - Welder qualification by procedure tests.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 2 2012-10-01 2012-10-01 false Welder qualification by procedure tests. 57.05-4 Section 57.05-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-4 Welder qualification by procedure tests...

  4. 46 CFR 57.05-4 - Welder qualification by procedure tests.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 2 2011-10-01 2011-10-01 false Welder qualification by procedure tests. 57.05-4 Section 57.05-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING AND BRAZING Performance Qualifications § 57.05-4 Welder qualification by procedure tests...

  5. SRS H1616 hydride transport vessel qualification report

    DOT National Transportation Integrated Search

    1998-12-01

    This report serves as the design qualification basis for both transport and facility use. headings identify report sections as containing qualification information for transport use, facility use, or both transport and facility use. This report demon...

  6. Test report: Electron-proton spectrometer qualification test unit, qualification test

    NASA Technical Reports Server (NTRS)

    Vincent, D. L.

    1972-01-01

    Qualification tests of the electron-proton spectrometer test unit are presented. The tests conducted were: (1) functional, (2) thermal/vacuum, (3) electromagnetic interference, (4) acoustic, (5) shock, (6) vibration, and (7) humidity. Results of each type of test are presented in the form of data sheets.

  7. An automation-assisted generic approach for biological sample preparation and LC-MS/MS method validation.

    PubMed

    Zhang, Jie; Wei, Shimin; Ayres, David W; Smith, Harold T; Tse, Francis L S

    2011-09-01

    Although it is well known that automation can provide significant improvement in the efficiency of biological sample preparation in quantitative LC-MS/MS analysis, it has not been widely implemented in bioanalytical laboratories throughout the industry. This can be attributed to the lack of a sound strategy and practical procedures in working with robotic liquid-handling systems. Several comprehensive automation assisted procedures for biological sample preparation and method validation were developed and qualified using two types of Hamilton Microlab liquid-handling robots. The procedures developed were generic, user-friendly and covered the majority of steps involved in routine sample preparation and method validation. Generic automation procedures were established as a practical approach to widely implement automation into the routine bioanalysis of samples in support of drug-development programs.

  8. Qualifications Frameworks in Africa: A Critical Reflection

    ERIC Educational Resources Information Center

    Higgs, P.; Keevy, J.

    2009-01-01

    Today there is an accelerating trend towards qualifications frameworks as an instrument to develop, classify and recognise formal learning across the African continent, as is also the case across most of Europe, Australasia and the Asia-Pacific region. As more and more countries and regions across the world develop qualifications frameworks to…

  9. A 96-well screen filter plate for high-throughput biological sample preparation and LC-MS/MS analysis.

    PubMed

    Peng, Sean X; Cousineau, Martin; Juzwin, Stephen J; Ritchie, David M

    2006-01-01

    A novel 96-well screen filter plate (patent pending) has been invented to eliminate a time-consuming and labor-intensive step in preparation of in vivo study samples--to remove blood or plasma clots. These clots plug the pipet tips during a manual or automated sample-transfer step causing inaccurate pipetting or total pipetting failure. Traditionally, these blood and plasma clots are removed by picking them out manually one by one from each sample tube before any sample transfer can be made. This has significantly slowed the sample preparation process and has become a bottleneck for automated high-throughput sample preparation using robotic liquid handlers. Our novel screen filter plate was developed to solve this problem. The 96-well screen filter plate consists of 96 stainless steel wire-mesh screen tubes connected to the 96 openings of a top plate so that the screen filter plate can be readily inserted into a 96-well sample storage plate. Upon insertion, the blood and plasma clots are excluded from entering the screen tube while clear sample solutions flow freely into it. In this way, sample transfer can be easily completed by either manual or automated pipetting methods. In this report, three structurally diverse compounds were selected to evaluate and validate the use of the screen filter plate. The plasma samples of these compounds were transferred and processed in the presence and absence of the screen filter plate and then analyzed by LC-MS/MS methods. Our results showed a good agreement between the samples prepared with and without the screen filter plate, demonstrating the utility and efficiency of this novel device for preparation of blood and plasma samples. The device is simple, easy to use, and reusable. It can be employed for sample preparation of other biological fluids that contain floating particulates or aggregates.

  10. Equipment for Topographical Preparation and Analysis of Various Semiconductor Infrared Detector Samples

    DTIC Science & Technology

    2015-11-13

    P Wijewarnasuriya at the Army Research Lab to understand the bandd offsets of HgCdTe infrared detector structures. Especially when a sample is not...Final Report: Equipment for Topographical Preparation and Analysis of Various Semiconductor Infrared Detector Samples Report Title A used calibrated...structures i. G15-38 and G15-38 Quantum Dot ---------------------------- 16 Infrared Detector Samples ii. GSU13-MPD-GB1 Heterostructure

  11. 49 CFR 214.347 - Training and qualification for lone workers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Training and qualification for lone workers. 214... § 214.347 Training and qualification for lone workers. Each lone worker shall be trained and qualified... training and qualification for lone workers shall include, as a minimum, consideration of the following...

  12. 49 CFR 214.347 - Training and qualification for lone workers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Training and qualification for lone workers. 214... § 214.347 Training and qualification for lone workers. Each lone worker shall be trained and qualified... training and qualification for lone workers shall include, as a minimum, consideration of the following...

  13. Sample preparation composite and replicate strategy case studies for assay of solid oral drug products.

    PubMed

    Nickerson, Beverly; Harrington, Brent; Li, Fasheng; Guo, Michele Xuemei

    2017-11-30

    Drug product assay is one of several tests required for new drug products to ensure the quality of the product at release and throughout the life cycle of the product. Drug product assay testing is typically performed by preparing a composite sample of multiple dosage units to obtain an assay value representative of the batch. In some cases replicate composite samples may be prepared and the reportable assay value is the average value of all the replicates. In previously published work by Harrington et al. (2014) [5], a sample preparation composite and replicate strategy for assay was developed to provide a systematic approach which accounts for variability due to the analytical method and dosage form with a standard error of the potency assay criteria based on compendia and regulatory requirements. In this work, this sample preparation composite and replicate strategy for assay is applied to several case studies to demonstrate the utility of this approach and its application at various stages of pharmaceutical drug product development. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. First experiment on LMJ facility: pointing and synchronisation qualification, sequences qualification

    NASA Astrophysics Data System (ADS)

    Henry, Olivier; Raffestin, Didier; Bretheau, Dominique; Luttmann, Michel; Graillot, Herve; Ferri, Michel; Seguineau, Frederic; Bar, Emmanuel; Patissou, Loic; Canal, Philippe; Sautarel, Françoise; Tranquille-Marques, Yves

    2015-11-01

    The LMJ (Laser mega Joule) facility at the CESTA site (Aquitaine, France) is a tool designed to deliver up to 1.2 MJ at 351 nm. The experiment system will include plasma diagnostics: UV and X energy balances, imagers (Streak and stripe camera, CCD), spectrometers, and a Visar/pyrometer. The facility must be able to deliver, within the hour following the shot, all the results of the plasma diagnostics, alignment images and laser diagnostic measurements. Part of the end of 2014 was devoted to the qualification of system pointing on target and synchronization within and between beams. The shots made with one chain (divided in 2 quads - 8 laser beams) have achieved 50 μm of misalignment accuracy and a synchronization accuracy in the order of 50 ps. The performances achieved for plasma diagnostic (in the order of less 100 μm of alignment and timing accuracy less than 150 ps) comply with expectations. At the same time the first automatic sequences were tested. They allowed a shot on target every 6h:30 and in some case twice a day by reducing preparation actions, leading to a sequence of 4h:00. These shooting sequences are managed by an operating team of 7 people helped by 3 people for security aspects.

  15. Implementing a National Qualifications Framework in Lithuania

    ERIC Educational Resources Information Center

    Tutlys, Vidmantas; Spudyte, Irma

    2011-01-01

    The design of the national qualifications framework (NQF) in Lithuania started in 2006. The NQF was officially approved by the government decree in May 2010. This article explores the influence of the processes of institutional change on the reform of the national system of qualifications in Lithuania through the implementation of the NQF, looking…

  16. Job Outcomes among Korean Graduates with Vocational Qualifications

    ERIC Educational Resources Information Center

    Lee, Young-Min; Cho, Sung-Eun

    2017-01-01

    Purpose: The purpose of this paper is to ascertain the status of recent Korean four-year college students' acquisition of vocational qualification certificates (hereinafter "certificates" or "qualifications"), the factors that influence certificate acquisition, and the resulting employment and wage effects.…

  17. Sample collection and preparation of biofluids and extracts for gas chromatography-mass spectrometry.

    PubMed

    Emwas, Abdul-Hamid M; Al-Talla, Zeyad A; Kharbatia, Najeh M

    2015-01-01

    To maximize the utility of gas chromatography-mass spectrometry (GC-MS) in metabonomics research, all stages of the experimental design should be standardized, including sample collection, storage, preparation, and sample separation. Moreover, the prerequisite for any GC-MS analysis is that a compound must be volatile and thermally stable if it is to be analyzed using this technique. Since many metabolites are nonvolatile and polar in nature, they are not readily amenable to analysis by GC-MS and require initial chemical derivatization of the polar functional groups in order to reduce the polarity and to increase the thermal stability and volatility of the analytes. In this chapter, an overview is presented of the optimum approach to sample collection, storage, and preparation for gas chromatography-mass spectrometry-based metabonomics with particular focus on urine samples as example of biofluids.

  18. 42 CFR 493.1423 - Standard; Testing personnel qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 5 2013-10-01 2013-10-01 false Standard; Testing personnel qualifications. 493... Testing Laboratories Performing Moderate Complexity Testing § 493.1423 Standard; Testing personnel qualifications. Each individual performing moderate complexity testing must— (a) Possess a current license issued...

  19. 42 CFR 493.1423 - Standard; Testing personnel qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 5 2011-10-01 2011-10-01 false Standard; Testing personnel qualifications. 493... Testing Laboratories Performing Moderate Complexity Testing § 493.1423 Standard; Testing personnel qualifications. Each individual performing moderate complexity testing must— (a) Possess a current license issued...

  20. 42 CFR 493.1423 - Standard; Testing personnel qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 5 2014-10-01 2014-10-01 false Standard; Testing personnel qualifications. 493... Testing Laboratories Performing Moderate Complexity Testing § 493.1423 Standard; Testing personnel qualifications. Each individual performing moderate complexity testing must— (a) Possess a current license issued...

  1. 42 CFR 493.1423 - Standard; Testing personnel qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 5 2012-10-01 2012-10-01 false Standard; Testing personnel qualifications. 493... Testing Laboratories Performing Moderate Complexity Testing § 493.1423 Standard; Testing personnel qualifications. Each individual performing moderate complexity testing must— (a) Possess a current license issued...

  2. 9 CFR 147.8 - Procedures for preparing egg yolk samples for diagnostic tests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Procedures for preparing egg yolk... IMPROVEMENT PLAN Blood Testing Procedures § 147.8 Procedures for preparing egg yolk samples for diagnostic... chapter. (a) Under the supervision of an Authorized Agent or State Inspector, the eggs which are used in...

  3. The Big-Five Personality Traits, Maternal Smoking during Pregnancy, and Educational Qualifications as Predictors of Tobacco Use in a Nationally Representative Sample.

    PubMed

    Cheng, Helen; Furnham, Adrian

    2016-01-01

    To investigate the associations between the Big-Five personality traits, parental social class, maternal smoking status during pregnancy, childhood cognitive ability, education and occupation, and tobacco use in a longitudinal birth cohort study. 17,415 babies born in Great Britain in 1958 and followed up at 11, 33, and 50 years of age. Lifelong tobacco use status (ever/never) and current tobacco use status (yes/no) at age 50 years were the outcome measures respectively. Logistic regression analyses showed that among the 5,840 participants with complete data, whilst maternal smoking status, educational qualifications, and all the big-5 personality traits were significant predictors of adult lifelong tobacco use; educational qualifications, own occupational levels, traits Extraversion, Conscientiousness, and Openness were significant predictors of current smoking status. In lifelong measure men tended to have a greater rate of tobacco use than women (52.1% in men and 49.2% in women). However, the sex effect on lifelong tobacco use ceased to be significant once a set of socio-economic and psychological variables in childhood and adulthood were taken into account. Educational qualifications and the Big-Five personality traits were significantly associated with both current and lifelong tobacco use status.

  4. Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography.

    PubMed

    Trépout, Sylvain; Bastin, Philippe; Marco, Sergio

    2017-03-12

    This report describes a protocol for preparing thick biological specimens for further observation using a scanning transmission electron microscope. It also describes an imaging method for studying the 3D structure of thick biological specimens by scanning transmission electron tomography. The sample preparation protocol is based on conventional methods in which the sample is fixed using chemical agents, treated with a heavy atom salt contrasting agent, dehydrated in a series of ethanol baths, and embedded in resin. The specific imaging conditions for observing thick samples by scanning transmission electron microscopy are then described. Sections of the sample are observed using a through-focus method involving the collection of several images at various focal planes. This enables the recovery of in-focus information at various heights throughout the sample. This particular collection pattern is performed at each tilt angle during tomography data collection. A single image is then generated, merging the in-focus information from all the different focal planes. A classic tilt-series dataset is then generated. The advantage of the method is that the tilt-series alignment and reconstruction can be performed using standard tools. The collection of through-focal images allows the reconstruction of a 3D volume that contains all of the structural details of the sample in focus.

  5. 10 CFR 603.515 - Qualification of a consortium.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS TECHNOLOGY INVESTMENT AGREEMENTS Pre-Award Business Evaluation Recipient Qualification § 603.515 Qualification of a consortium. (a) A consortium that... under the agreement. (b) If the prospective recipient of a TIA is a consortium that is not formally...

  6. 10 CFR 603.515 - Qualification of a consortium.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS TECHNOLOGY INVESTMENT AGREEMENTS Pre-Award Business Evaluation Recipient Qualification § 603.515 Qualification of a consortium. (a) A consortium that... under the agreement. (b) If the prospective recipient of a TIA is a consortium that is not formally...

  7. 10 CFR 603.515 - Qualification of a consortium.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS TECHNOLOGY INVESTMENT AGREEMENTS Pre-Award Business Evaluation Recipient Qualification § 603.515 Qualification of a consortium. (a) A consortium that... under the agreement. (b) If the prospective recipient of a TIA is a consortium that is not formally...

  8. 29 CFR 1926.1076 - Qualifications of dive team.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 8 2011-07-01 2011-07-01 false Qualifications of dive team. 1926.1076 Section 1926.1076 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... § 1926.1076 Qualifications of dive team. Note: The requirements applicable to construction work under...

  9. 29 CFR 1926.1076 - Qualifications of dive team.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 8 2010-07-01 2010-07-01 false Qualifications of dive team. 1926.1076 Section 1926.1076 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... § 1926.1076 Qualifications of dive team. Note: The requirements applicable to construction work under...

  10. 29 CFR 1926.1076 - Qualifications of dive team.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 8 2013-07-01 2013-07-01 false Qualifications of dive team. 1926.1076 Section 1926.1076 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... § 1926.1076 Qualifications of dive team. Note: The requirements applicable to construction work under...

  11. 29 CFR 1926.1076 - Qualifications of dive team.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 8 2014-07-01 2014-07-01 false Qualifications of dive team. 1926.1076 Section 1926.1076 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... § 1926.1076 Qualifications of dive team. Note: The requirements applicable to construction work under...

  12. 29 CFR 1926.1076 - Qualifications of dive team.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 8 2012-07-01 2012-07-01 false Qualifications of dive team. 1926.1076 Section 1926.1076 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... § 1926.1076 Qualifications of dive team. Note: The requirements applicable to construction work under...

  13. 14 CFR 121.439 - Pilot qualification: Recent experience.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Pilot qualification: Recent experience. 121.439 Section 121.439 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Pilot qualification: Recent experience. (a) No certificate holder may use any person nor may any person...

  14. 14 CFR 121.439 - Pilot qualification: Recent experience.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Pilot qualification: Recent experience. 121.439 Section 121.439 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Pilot qualification: Recent experience. (a) No certificate holder may use any person nor may any person...

  15. 14 CFR 125.285 - Pilot qualifications: Recent experience.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Pilot qualifications: Recent experience. 125.285 Section 125.285 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Requirements § 125.285 Pilot qualifications: Recent experience. (a) No certificate holder may use any person...

  16. 14 CFR 125.285 - Pilot qualifications: Recent experience.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Pilot qualifications: Recent experience. 125.285 Section 125.285 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Requirements § 125.285 Pilot qualifications: Recent experience. (a) No certificate holder may use any person...

  17. 14 CFR 125.285 - Pilot qualifications: Recent experience.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Pilot qualifications: Recent experience. 125.285 Section 125.285 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Requirements § 125.285 Pilot qualifications: Recent experience. (a) No certificate holder may use any person...

  18. 32 CFR 571.2 - Basic qualifications for enlistment.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 3 2014-07-01 2014-07-01 false Basic qualifications for enlistment. 571.2 Section 571.2 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY PERSONNEL RECRUITING AND ENLISTMENTS Recruiting and Enlistment Eligibility § 571.2 Basic qualifications for enlistment...

  19. 10 CFR 61.61 - Applicant qualifications and assurances.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 2 2013-01-01 2013-01-01 false Applicant qualifications and assurances. 61.61 Section 61.61 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR LAND DISPOSAL OF RADIOACTIVE WASTE Financial Assurances § 61.61 Applicant qualifications and assurances. Each applicant shall...

  20. 10 CFR 61.61 - Applicant qualifications and assurances.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 2 2014-01-01 2014-01-01 false Applicant qualifications and assurances. 61.61 Section 61.61 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR LAND DISPOSAL OF RADIOACTIVE WASTE Financial Assurances § 61.61 Applicant qualifications and assurances. Each applicant shall...

  1. 10 CFR 61.61 - Applicant qualifications and assurances.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Applicant qualifications and assurances. 61.61 Section 61.61 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR LAND DISPOSAL OF RADIOACTIVE WASTE Financial Assurances § 61.61 Applicant qualifications and assurances. Each applicant shall...

  2. 10 CFR 61.61 - Applicant qualifications and assurances.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 2 2012-01-01 2012-01-01 false Applicant qualifications and assurances. 61.61 Section 61.61 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR LAND DISPOSAL OF RADIOACTIVE WASTE Financial Assurances § 61.61 Applicant qualifications and assurances. Each applicant shall...

  3. 10 CFR 61.61 - Applicant qualifications and assurances.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 2 2011-01-01 2011-01-01 false Applicant qualifications and assurances. 61.61 Section 61.61 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR LAND DISPOSAL OF RADIOACTIVE WASTE Financial Assurances § 61.61 Applicant qualifications and assurances. Each applicant shall...

  4. Thermal protection system development, testing, and qualification for atmospheric probes and sample return missions. Examples for Saturn, Titan and Stardust-type sample return

    NASA Astrophysics Data System (ADS)

    Venkatapathy, E.; Laub, B.; Hartman, G. J.; Arnold, J. O.; Wright, M. J.; Allen, G. A.

    2009-07-01

    The science community has continued to be interested in planetary entry probes, aerocapture, and sample return missions to improve our understanding of the Solar System. As in the case of the Galileo entry probe, such missions are critical to the understanding not only of the individual planets, but also to further knowledge regarding the formation of the Solar System. It is believed that Saturn probes to depths corresponding to 10 bars will be sufficient to provide the desired data on its atmospheric composition. An aerocapture mission would enable delivery of a satellite to provide insight into how gravitational forces cause dynamic changes in Saturn's ring structure that are akin to the evolution of protoplanetary accretion disks. Heating rates for the "shallow" Saturn probes, Saturn aerocapture, and sample Earth return missions with higher re-entry speeds (13-15 km/s) from Mars, Venus, comets, and asteroids are in the range of 1-6 KW/cm 2. New, mid-density thermal protection system (TPS) materials for such probes can be mission enabling for mass efficiency and also for use on smaller vehicles enabled by advancements in scientific instrumentation. Past consideration of new Jovian multiprobe missions has been considered problematic without the Giant Planet arcjet facility that was used to qualify carbon phenolic for the Galileo probe. This paper describes emerging TPS technologies and the proposed use of an affordable, small 5 MW arcjet that can be used for TPS development, in test gases appropriate for future planetary probe and aerocapture applications. Emerging TPS technologies of interest include new versions of the Apollo Avcoat material and a densified variant of Phenolic Impregnated Carbon Ablator (PICA). Application of these and other TPS materials and the use of other facilities for development and qualification of TPS for Saturn, Titan, and Sample Return missions of the Stardust class with entry speeds from 6.0 to 28.6 km/s are discussed.

  5. Open-Source RTOS Space Qualification: An RTEMS Case Study

    NASA Technical Reports Server (NTRS)

    Zemerick, Scott

    2017-01-01

    NASA space-qualification of reusable off-the-shelf real-time operating systems (RTOSs) remains elusive due to several factors notably (1) The diverse nature of RTOSs utilized across NASA, (2) No single NASA space-qualification criteria, lack of verification and validation (V&V) analysis, or test beds, and (3) different RTOS heritages, specifically open-source RTOSs and closed vendor-provided RTOSs. As a leader in simulation test beds, the NASA IV&V Program is poised to help jump-start and lead the space-qualification effort of the open source Real-Time Executive for Multiprocessor Systems (RTEMS) RTOS. RTEMS, as a case-study, can be utilized as an example of how to qualify all RTOSs, particularly the reusable non-commercial (open-source) ones that are gaining usage and popularity across NASA. Qualification will improve the overall safety and mission assurance of RTOSs for NASA-agency wide usage. NASA's involvement in space-qualification of an open-source RTOS such as RTEMS will drive the RTOS industry toward a more qualified and mature open-source RTOS product.

  6. Transformation of Learning in Education and Training: Key Qualifications Revisited. CEDEFOP Reference Series.

    ERIC Educational Resources Information Center

    Kamarainen, Pekka, Ed.; Attwell, Graham, Ed.; Brown, Alan, Ed.

    This book contains 15 papers examining European approaches to the theme of key qualifications. The following papers are included: "Key Qualifications Revisited: An Introduction" (Pekka Kamarainen); "Exploring Key Qualifications: Context, Theory, and Practice in Europe" (Pekka Kamarainen); "Rethinking Key Qualifications:…

  7. 49 CFR 240.405 - Processing qualification review petitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Processing qualification review petitions. 240.405... Resolution Procedures § 240.405 Processing qualification review petitions. (a) Each petition shall be acknowledged in writing by FRA. The acknowledgment shall contain the docket number assigned to the petition and...

  8. 27 CFR 19.823 - Changes after original qualification.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Changes after original... Vaporizing Process Qualification Documents § 19.823 Changes after original qualification. When there is a change in the information recorded in the original approved application, the proprietor shall make a...

  9. 14 CFR 135.247 - Pilot qualifications: Recent experience.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Pilot qualifications: Recent experience... Flight Crewmember Requirements § 135.247 Pilot qualifications: Recent experience. (a) No certificate holder may use any person, nor may any person serve, as pilot in command of an aircraft carrying...

  10. 14 CFR 135.247 - Pilot qualifications: Recent experience.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Pilot qualifications: Recent experience... Flight Crewmember Requirements § 135.247 Pilot qualifications: Recent experience. (a) No certificate holder may use any person, nor may any person serve, as pilot in command of an aircraft carrying...

  11. 29 CFR 452.53 - Application of qualifications for office.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requirement. A qualification which is not part of the constitution and bylaws or other duly enacted rules of... Federal or State law. 32 Qualifications must be specific and objective. They must contain specific...

  12. 29 CFR 452.53 - Application of qualifications for office.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... requirement. A qualification which is not part of the constitution and bylaws or other duly enacted rules of... Federal or State law. 32 Qualifications must be specific and objective. They must contain specific...

  13. School Leadership Preparation in Malaysia: Aims, Content and Impact

    ERIC Educational Resources Information Center

    Ng, Ashley Yoon-Mooi

    2017-01-01

    This paper examines the preparation of school principals in Malaysia, and the aspiration of the Malaysian Education Blueprint 2013-2025 to ensure high-performing school leaders in every school. It reports on the principal preparatory programme, the National Professional Qualification for Educational Leaders, which is mandatory to those who aspire…

  14. The planning and establishment of a sample preparation laboratory for drug discovery

    PubMed Central

    Dufresne, Claude

    2000-01-01

    Nature has always been a productive source of new drugs. With the advent of high-throughput screening, it has now become possible to rapidly screen large sample collections. In addition to seeking greater diversity from natural product sources (micro-organisms, plants, etc.), fractionation of the crude extracts prior to screening is becoming a more important part of our efforts. As sample preparation protocols become more involved, automation can help to achieve and maintain a desired sample throughput. To address the needs of our screening program, two robotic systems were designed. The first system processes crude extracts all the way to 96-well plates, containing solutions suitable for screening in biological and biochemical assays. The system can dissolve crude extracts, fractionate them on solid-phase extraction cartridges, dry and weigh each fraction, re-dissolve them to a known concentration, and prepare mother plates. The second system replicates mother plates into a number of daughter plates. PMID:18924691

  15. La 2-xSr xCuO 4-δ superconducting samples prepared by the wet-chemical method

    NASA Astrophysics Data System (ADS)

    Loose, A.; Gonzalez, J. L.; Lopez, A.; Borges, H. A.; Baggio-Saitovitch, E.

    2009-10-01

    In this work, we report on the physical properties of good-quality polycrystalline superconducting samples of La 2-xSr xCu 1-yZn yO 4-δ ( y=0, 0.02) prepared by a wet-chemical method, focusing on the temperature dependence of the critical current. Using the wet-chemical method, we were able to produce samples with improved homogeneity compared to the solid-state method. A complete set of samples with several carrier concentrations, ranging from the underdoped (strontium concentration x≈0.05) to the highly overdoped ( x≈0.25) region, were prepared and investigated. The X-ray diffraction analysis, zero-field cooling magnetization and electrical resistivity measurements were reported on earlier. The structural parameters of the prepared samples seem to be slightly modified by the preparation method and their critical temperatures were lower than reported in the literature. The temperature dependence of the critical current was explained by a theoretical model which took the granular structure of the samples into account.

  16. Preparation and validation of gross alpha/beta samples used in EML`s quality assessment program

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Scarpitta, S.C.

    1997-10-01

    A set of water and filter samples have been incorporated into the existing Environmental Measurements Laboratory`s (EML) Quality Assessment Program (QAP) for gross alpha/beta determinations by participating DOE laboratories. The participating laboratories are evaluated by comparing their results with the EML value. The preferred EML method for measuring water and filter samples, described in this report, uses gas flow proportional counters with 2 in. detectors. Procedures for sample preparation, quality control and instrument calibration are presented. Liquid scintillation (LS) counting is an alternative technique that is suitable for quantifying both the alpha ({sup 241}Am, {sup 230}Th and {sup 238}Pu) andmore » beta ({sup 90}Sr/{sup 90}Y) activity concentrations in the solutions used to prepare the QAP water and air filter samples. Three LS counting techniques (Cerenkov, dual dpm and full spectrum analysis) are compared. These techniques may be used to validate the activity concentrations of each component in the alpha/beta solution before the QAP samples are actually prepared.« less

  17. Qualification Users' Perceptions and Experiences of Assessment Reliability

    ERIC Educational Resources Information Center

    Chamberlain, Suzanne

    2013-01-01

    This paper presents the findings of a study designed to explore qualification users' perceptions and experiences of reliability in the context of national assessment outcomes in England. The study consisted of 17 focus groups conducted across six sectors of qualification users: students, teachers, trainee teachers, job-seekers, employers and…

  18. 30 CFR 75.155 - Qualified hoisting engineer; qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...-hoisting engineer; or (2) If a State has no program for qualifying persons as electric-hoisting engineers... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Qualified hoisting engineer; qualifications. 75... Persons § 75.155 Qualified hoisting engineer; qualifications. (a)(1) A person is a qualified hoisting...

  19. 30 CFR 75.155 - Qualified hoisting engineer; qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...-hoisting engineer; or (2) If a State has no program for qualifying persons as electric-hoisting engineers... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Qualified hoisting engineer; qualifications. 75... Persons § 75.155 Qualified hoisting engineer; qualifications. (a)(1) A person is a qualified hoisting...

  20. 30 CFR 75.155 - Qualified hoisting engineer; qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...-hoisting engineer; or (2) If a State has no program for qualifying persons as electric-hoisting engineers... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Qualified hoisting engineer; qualifications. 75... Persons § 75.155 Qualified hoisting engineer; qualifications. (a)(1) A person is a qualified hoisting...

  1. 30 CFR 75.155 - Qualified hoisting engineer; qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...-hoisting engineer; or (2) If a State has no program for qualifying persons as electric-hoisting engineers... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Qualified hoisting engineer; qualifications. 75... Persons § 75.155 Qualified hoisting engineer; qualifications. (a)(1) A person is a qualified hoisting...

  2. 30 CFR 75.155 - Qualified hoisting engineer; qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...-hoisting engineer; or (2) If a State has no program for qualifying persons as electric-hoisting engineers... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Qualified hoisting engineer; qualifications. 75... Persons § 75.155 Qualified hoisting engineer; qualifications. (a)(1) A person is a qualified hoisting...

  3. Uptake and Utility of VET Qualifications. Research Report

    ERIC Educational Resources Information Center

    Korbel, Patrick; Misko, Josie

    2016-01-01

    Training packages and accredited courses are the core training products of the nationally accredited vocational education and training (VET) system in Australia. This report considers the use of these training packages and the qualifications contained within them. The report also examines the pattern of enrolments in qualifications to determine…

  4. 14 CFR 121.437 - Pilot qualification: Certificates required.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Pilot qualification: Certificates required... Pilot qualification: Certificates required. (a) No pilot may act as pilot in command of an aircraft (or... pilots) unless he holds an airline transport pilot certificate and an appropriate type rating for that...

  5. 14 CFR 121.437 - Pilot qualification: Certificates required.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Pilot qualification: Certificates required... Pilot qualification: Certificates required. (a) No pilot may act as pilot in command of an aircraft (or... pilots) unless he holds an airline transport pilot certificate and an appropriate type rating for that...

  6. 14 CFR 125.285 - Pilot qualifications: Recent experience.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Pilot qualifications: Recent experience... Requirements § 125.285 Pilot qualifications: Recent experience. (a) No certificate holder may use any person, nor may any person serve, as a required pilot flight crewmember unless within the preceding 90...

  7. 14 CFR 125.285 - Pilot qualifications: Recent experience.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Pilot qualifications: Recent experience... Requirements § 125.285 Pilot qualifications: Recent experience. (a) No certificate holder may use any person, nor may any person serve, as a required pilot flight crewmember unless within the preceding 90...

  8. 12 CFR 193.3 - Qualification of public accountant.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 1 2013-01-01 2013-01-01 false Qualification of public accountant. 193.3... REQUIREMENTS Form and Content of Financial Statements § 193.3 Qualification of public accountant. The term “qualified public accountant” means a certified public accountant or licensed public accountant certified or...

  9. 12 CFR 193.3 - Qualification of public accountant.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 1 2014-01-01 2014-01-01 false Qualification of public accountant. 193.3... REQUIREMENTS Form and Content of Financial Statements § 193.3 Qualification of public accountant. The term “qualified public accountant” means a certified public accountant or licensed public accountant certified or...

  10. 12 CFR 193.3 - Qualification of public accountant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 1 2012-01-01 2012-01-01 false Qualification of public accountant. 193.3... REQUIREMENTS Form and Content of Financial Statements § 193.3 Qualification of public accountant. The term “qualified public accountant” means a certified public accountant or licensed public accountant certified or...

  11. 42 CFR 410.77 - Certified nurse-midwives' services: Qualifications and conditions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 2 2010-10-01 2010-10-01 false Certified nurse-midwives' services: Qualifications... Medical and Other Health Services § 410.77 Certified nurse-midwives' services: Qualifications and conditions. (a) Qualifications. For Medicare coverage of his or her services, a certified nurse-midwife must...

  12. 42 CFR 410.77 - Certified nurse-midwives' services: Qualifications and conditions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 2 2011-10-01 2011-10-01 false Certified nurse-midwives' services: Qualifications... Medical and Other Health Services § 410.77 Certified nurse-midwives' services: Qualifications and conditions. (a) Qualifications. For Medicare coverage of his or her services, a certified nurse-midwife must...

  13. 29 CFR 1918.98 - Qualifications of machinery operators and supervisory training.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 7 2013-07-01 2013-07-01 false Qualifications of machinery operators and supervisory... General Working Conditions. § 1918.98 Qualifications of machinery operators and supervisory training. (a) Qualification of machinery operators. (1) Only an employee determined by the employer to be competent by reason...

  14. 29 CFR 1918.98 - Qualifications of machinery operators and supervisory training.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 7 2012-07-01 2012-07-01 false Qualifications of machinery operators and supervisory... General Working Conditions. § 1918.98 Qualifications of machinery operators and supervisory training. (a) Qualification of machinery operators. (1) Only an employee determined by the employer to be competent by reason...

  15. 29 CFR 1918.98 - Qualifications of machinery operators and supervisory training.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 7 2014-07-01 2014-07-01 false Qualifications of machinery operators and supervisory... General Working Conditions. § 1918.98 Qualifications of machinery operators and supervisory training. (a) Qualification of machinery operators. (1) Only an employee determined by the employer to be competent by reason...

  16. 29 CFR 1918.98 - Qualifications of machinery operators and supervisory training.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 7 2011-07-01 2011-07-01 false Qualifications of machinery operators and supervisory... General Working Conditions. § 1918.98 Qualifications of machinery operators and supervisory training. (a) Qualification of machinery operators. (1) Only an employee determined by the employer to be competent by reason...

  17. BSM Delta Qualification 2, volume 3, book 2

    NASA Technical Reports Server (NTRS)

    1994-01-01

    This report, presented in three volumes, provides the results of a two-motor Delta Qualification 2 program conducted in 1993 to certify the following enhancements for incorporation into booster separation motor (BSM0 flight hardware: vulcanized-in-place nozzle aft closure insulation; new iso-static ATJ bulk graphite throat insert material, adhesive EA9394 for bonding the nozzle throat, igniter grain rod/centering insert/igniter case; deletion of the igniter adapter insulator ring; deletion of the igniter adapter/igniter case interface RTV; and deletion of loctite from igniter retainer plate threads. The enhancements above directly resulted from (1) the BSM total quality management (TQM) team initiatives to enhance the BSM producibility, and (2) the necessity to qualify new throat insert and adhesive systems to replace existing materials that will not be available. Testing was completed at both the component and motor levels. Component testing was accomplished to screen candidate materials (e.g., throat materials, adhesive systems) and to optimize processes (e.g., aft closure insulator vulcanization approach) prior to their incorporation into the test motors. Motor testing--consisting of two motors, randomly selected by USBI's on-site quality personnel from production lot AAY, which were modified to accept the enhancements -- was completed to provide the final qualification of the enhancements for incorporation into flight hardware. Volume 3, Book 2 provides various supporting documentation to the previous volumes with regards to the testing of the two Delta qualification units: data acceptance records, thermal conditioning analysis, igniter adapter thermal flake analysis, laboratory adhesive (EA-9394) qualification report, throat insert thermal/structural analysis, Delta Qualification Nonconformance Reports (NCR's), O-ring seating tests, and interim test report for vulcanization process qualification.

  18. Transmission Electron Microscopy (TEM) Sample Preparation of Si(1-x)Gex in c-Plane Sapphire Substrate

    NASA Technical Reports Server (NTRS)

    Kim, Hyun Jung; Choi, Sang H.; Bae, Hyung-Bin; Lee, Tae Woo

    2012-01-01

    The National Aeronautics and Space Administration-invented X-ray diffraction (XRD) methods, including the total defect density measurement method and the spatial wafer mapping method, have confirmed super hetero epitaxy growth for rhombohedral single crystalline silicon germanium (Si1-xGex) on a c-plane sapphire substrate. However, the XRD method cannot observe the surface morphology or roughness because of the method s limited resolution. Therefore the authors used transmission electron microscopy (TEM) with samples prepared in two ways, the focused ion beam (FIB) method and the tripod method to study the structure between Si1-xGex and sapphire substrate and Si1?xGex itself. The sample preparation for TEM should be as fast as possible so that the sample should contain few or no artifacts induced by the preparation. The standard sample preparation method of mechanical polishing often requires a relatively long ion milling time (several hours), which increases the probability of inducing defects into the sample. The TEM sampling of the Si1-xGex on sapphire is also difficult because of the sapphire s high hardness and mechanical instability. The FIB method and the tripod method eliminate both problems when performing a cross-section TEM sampling of Si1-xGex on c-plane sapphire, which shows the surface morphology, the interface between film and substrate, and the crystal structure of the film. This paper explains the FIB sampling method and the tripod sampling method, and why sampling Si1-xGex, on a sapphire substrate with TEM, is necessary.

  19. Qualification of silicon pore optics

    NASA Astrophysics Data System (ADS)

    Wille, Eric; Bavdaz, Marcos; Fransen, Sebastiaan; Collon, Maximilien; Ackermann, Marcelo; Guenther, Ramses; Chatbi, Abdelhakim; Vacanti, Giuseppe; Vervest, Mark; van Baren, Coen; Haneveld, Jeroen; Riekerink, Mark Olde; Koelewijn, Arenda; Kampf, Dirk; Zuknik, Karl-Heinz; Reutlinger, Arnd

    2014-07-01

    Silicon Pore Optics (SPO) are the enabling technology for ESA's second large class mission in the Cosmic Vision programme. As for every space hardware, a critical qualification process is required to verify the suitability of the SPO mirror modules surviving the launch loads and maintaining their performance in the space environment. We present recent design modifications to further strengthen the mounting system (brackets and dowel pins) against mechanical loads. The progress of a formal qualification test campaign with the new mirror module design is shown. We discuss mechanical and thermal limitations of the SPO technology and provide recommendations for the mission design of the next X-ray Space Observatory.

  20. Preparation of pure microbiological samples for pyrolysis of gas-liquid chromatography studies.

    PubMed Central

    Oxborrow, G S; Fields, N D; Puleo, J R

    1976-01-01

    Bacterial samples were prepared for pyrolysis gas-liquid chromatography using cells grown on membrane filters. Pyrochromatograms were reproducible when cells harvested from the filters were pyrolyzed without being washed. Images PMID:970947

  1. National Vocational Qualifications in the United Kingdom: Their Origins and Legacy

    ERIC Educational Resources Information Center

    Young, Michael

    2011-01-01

    National Vocational Qualifications (NVQs) were launched in the United Kingdom (excluding Scotland) in 1987 as a framework for rationalising what was described at the time as the "jungle" of existing vocational qualifications. They were never intended to be the basis for a comprehensive NQF for all qualifications; however, successive…

  2. 32 CFR 935.139 - Motor vehicle operator qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Motor vehicle operator qualifications. 935.139... AND INSULAR REGULATIONS WAKE ISLAND CODE Motor Vehicle Code § 935.139 Motor vehicle operator qualifications. (a) No person may operate a privately owned motor vehicle on Wake Island unless he has an island...

  3. The Qualifications of Teachers in American High Schools.

    ERIC Educational Resources Information Center

    Pascal, Anthony

    This monograph describes the formal qualifications of the teachers currently teaching in public high schools and notes the variations of qualifications among schools with different characteristics. A survey of an average of 30 randomly selected teachers in each of 340 comprehensive high schools obtained information on: (1) the number of college…

  4. 32 CFR 935.139 - Motor vehicle operator qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Motor vehicle operator qualifications. 935.139... AND INSULAR REGULATIONS WAKE ISLAND CODE Motor Vehicle Code § 935.139 Motor vehicle operator qualifications. (a) No person may operate a privately owned motor vehicle on Wake Island unless he has an island...

  5. 14 CFR 121.439 - Pilot qualification: Recent experience.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Pilot qualification: Recent experience. 121... Pilot qualification: Recent experience. (a) No certificate holder may use any person nor may any person serve as a required pilot flight crewmember, unless within the preceding 90 days, that person has made...

  6. 14 CFR 121.439 - Pilot qualification: Recent experience.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Pilot qualification: Recent experience. 121... Pilot qualification: Recent experience. (a) No certificate holder may use any person nor may any person serve as a required pilot flight crewmember, unless within the preceding 90 days, that person has made...

  7. 12 CFR 390.382 - Qualification of public accountant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 5 2012-01-01 2012-01-01 false Qualification of public accountant. 390.382....382 Qualification of public accountant. (See also 17 CFR 210.2-01.) The term “qualified public accountant” means a certified public accountant or licensed public accountant certified or licensed by a...

  8. A comparison of sample preparation strategies for biological tissues and subsequent trace element analysis using LA-ICP-MS.

    PubMed

    Bonta, Maximilian; Török, Szilvia; Hegedus, Balazs; Döme, Balazs; Limbeck, Andreas

    2017-03-01

    Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) is one of the most commonly applied methods for lateral trace element distribution analysis in medical studies. Many improvements of the technique regarding quantification and achievable lateral resolution have been achieved in the last years. Nevertheless, sample preparation is also of major importance and the optimal sample preparation strategy still has not been defined. While conventional histology knows a number of sample pre-treatment strategies, little is known about the effect of these approaches on the lateral distributions of elements and/or their quantities in tissues. The technique of formalin fixation and paraffin embedding (FFPE) has emerged as the gold standard in tissue preparation. However, the potential use for elemental distribution studies is questionable due to a large number of sample preparation steps. In this work, LA-ICP-MS was used to examine the applicability of the FFPE sample preparation approach for elemental distribution studies. Qualitative elemental distributions as well as quantitative concentrations in cryo-cut tissues as well as FFPE samples were compared. Results showed that some metals (especially Na and K) are severely affected by the FFPE process, whereas others (e.g., Mn, Ni) are less influenced. Based on these results, a general recommendation can be given: FFPE samples are completely unsuitable for the analysis of alkaline metals. When analyzing transition metals, FFPE samples can give comparable results to snap-frozen tissues. Graphical abstract Sample preparation strategies for biological tissues are compared with regard to the elemental distributions and average trace element concentrations.

  9. Polished sample preparing and backscattered electron imaging and of fly ash-cement paste

    NASA Astrophysics Data System (ADS)

    Feng, Shuxia; Li, Yanqi

    2018-03-01

    In recent decades, the technology of backscattered electron imaging and image analysis was applied in more and more study of mixed cement paste because of its special advantages. Test accuracy of this technology is affected by polished sample preparation and image acquisition. In our work, effects of two factors in polished sample preparing and backscattered electron imaging were investigated. The results showed that increasing smoothing pressure could improve the flatness of polished surface and then help to eliminate interference of morphology on grey level distribution of backscattered electron images; increasing accelerating voltage was beneficial to increase gray difference among different phases in backscattered electron images.

  10. The Big-Five Personality Traits, Maternal Smoking during Pregnancy, and Educational Qualifications as Predictors of Tobacco Use in a Nationally Representative Sample

    PubMed Central

    Cheng, Helen; Furnham, Adrian

    2016-01-01

    Objective To investigate the associations between the Big-Five personality traits, parental social class, maternal smoking status during pregnancy, childhood cognitive ability, education and occupation, and tobacco use in a longitudinal birth cohort study. Method 17,415 babies born in Great Britain in 1958 and followed up at 11, 33, and 50 years of age. Lifelong tobacco use status (ever/never) and current tobacco use status (yes/no) at age 50 years were the outcome measures respectively. Results Logistic regression analyses showed that among the 5,840 participants with complete data, whilst maternal smoking status, educational qualifications, and all the big-5 personality traits were significant predictors of adult lifelong tobacco use; educational qualifications, own occupational levels, traits Extraversion, Conscientiousness, and Openness were significant predictors of current smoking status. In lifelong measure men tended to have a greater rate of tobacco use than women (52.1% in men and 49.2% in women). However, the sex effect on lifelong tobacco use ceased to be significant once a set of socio-economic and psychological variables in childhood and adulthood were taken into account. Conclusion Educational qualifications and the Big-Five personality traits were significantly associated with both current and lifelong tobacco use status. PMID:26731730

  11. The ExoMars Sample Preparation and Distribution System

    NASA Astrophysics Data System (ADS)

    Schulte, Wolfgang; Hofmann, Peter; Baglioni, Pietro; Richter, Lutz; Redlich, . Daniel; Notarnicola, Marco; Durrant, Stephen

    2012-07-01

    The Sample Preparation and Distribution System (SPDS) is a key element of the ESA ExoMars Rover. It is a set of complex mechanisms designed to receive Mars soil samples acquired from the subsurface with a drill, to crush them and to distribute the obtained soil powder to the scientific instruments of the `Pasteur Payload', in the Rover Analytical Laboratory (ALD). In particular, the SPDS consists of: (1) a Core Sample Handling System (CSHS), including a Core Sample Transportation Mechanism (CSTM) and a Blank Sample Dispenser; (2) a Crushing Station (CS); (3) a Powder Sample Dosing and Distribution System (PSDDS); and (4) a Powder Sample Handling System (PSHS) which is a carousel carrying pyrolysis ovens, a re-fillable sample container and a tool to flatten the powder sample surface. Kayser-Threde has developed, undercontract with the ExoMars prime contractor Thales Alenia Space Italy, breadboards and an engineering model of the SPDS mechanisms. Tests of individual mechanisms, namely the CSTM, CS and PSDDS were conducted both in laboratory ambient conditions and in a simulated Mars environment, using dedicated facilities. The SPDS functionalities and performances were measured and evaluated. In the course of 2011 the SPDS Dosing Station (part of the PSDDS) was also tested in simulated Mars gravity conditions during a parabolic flight campaign. By the time of the conference, an elegant breadboard of the Powder Sample Handling System will have been built and tested. The next step, planned by mid of 2012, will be a complete end-to-end test of the sample handling and processing chain, combining all four SPDS mechanisms. The possibility to verify interface and operational aspects between the SPDS and the ALD scientific instruments using the available instruments breadboards with the end-to-end set-up is currently being evaluated. This paper illustrates the most recent design status of the SPDS mechanisms, summarizes the test results and highlights future development

  12. 14 CFR 61.55 - Second-in-command qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Second-in-command qualifications. 61.55...-in-command qualifications. (a) A person may serve as a second-in-command of an aircraft type certificated for more than one required pilot flight crewmember or in operations requiring a second-in-command...

  13. 14 CFR 135.245 - Second in command qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Second in command qualifications. 135.245... Crewmember Requirements § 135.245 Second in command qualifications. (a) Except as provided in paragraph (b), no certificate holder may use any person, nor may any person serve, as second in command of an...

  14. 14 CFR 135.243 - Pilot in command qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Pilot in command qualifications. 135.243... Crewmember Requirements § 135.243 Pilot in command qualifications. (a) No certificate holder may use a person, nor may any person serve, as pilot in command in passenger-carrying operations— (1) Of a turbojet...

  15. 14 CFR 135.243 - Pilot in command qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Pilot in command qualifications. 135.243... Crewmember Requirements § 135.243 Pilot in command qualifications. (a) No certificate holder may use a person, nor may any person serve, as pilot in command in passenger-carrying operations— (1) Of a turbojet...

  16. 14 CFR 61.55 - Second-in-command qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Second-in-command qualifications. 61.55...-in-command qualifications. (a) A person may serve as a second-in-command of an aircraft type certificated for more than one required pilot flight crewmember or in operations requiring a second-in-command...

  17. 14 CFR 135.243 - Pilot in command qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Pilot in command qualifications. 135.243... Crewmember Requirements § 135.243 Pilot in command qualifications. (a) No certificate holder may use a person, nor may any person serve, as pilot in command in passenger-carrying operations— (1) Of a turbojet...

  18. 14 CFR 135.245 - Second in command qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Second in command qualifications. 135.245... Crewmember Requirements § 135.245 Second in command qualifications. (a) Except as provided in paragraph (b), no certificate holder may use any person, nor may any person serve, as second in command of an...

  19. 14 CFR 135.245 - Second in command qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Second in command qualifications. 135.245... Crewmember Requirements § 135.245 Second in command qualifications. (a) Except as provided in paragraph (b), no certificate holder may use any person, nor may any person serve, as second in command of an...

  20. 14 CFR 135.243 - Pilot in command qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Pilot in command qualifications. 135.243... Crewmember Requirements § 135.243 Pilot in command qualifications. (a) No certificate holder may use a person, nor may any person serve, as pilot in command in passenger-carrying operations— (1) Of a turbojet...

  1. 14 CFR 135.245 - Second in command qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Second in command qualifications. 135.245... Crewmember Requirements § 135.245 Second in command qualifications. (a) Except as provided in paragraph (b), no certificate holder may use any person, nor may any person serve, as second in command of an...

  2. 14 CFR 61.55 - Second-in-command qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Second-in-command qualifications. 61.55...-in-command qualifications. (a) A person may serve as a second-in-command of an aircraft type certificated for more than one required pilot flight crewmember or in operations requiring a second-in-command...

  3. 14 CFR 135.243 - Pilot in command qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Pilot in command qualifications. 135.243... Crewmember Requirements § 135.243 Pilot in command qualifications. (a) No certificate holder may use a person, nor may any person serve, as pilot in command in passenger-carrying operations— (1) Of a turbojet...

  4. 14 CFR 61.55 - Second-in-command qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Second-in-command qualifications. 61.55...-in-command qualifications. (a) A person may serve as a second-in-command of an aircraft type certificated for more than one required pilot flight crewmember or in operations requiring a second-in-command...

  5. 14 CFR 135.245 - Second in command qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Second in command qualifications. 135.245... Crewmember Requirements § 135.245 Second in command qualifications. (a) Except as provided in paragraph (b), no certificate holder may use any person, nor may any person serve, as second in command of an...

  6. The European Qualification Framework: Skills, Competences or Knowledge?

    ERIC Educational Resources Information Center

    Mehaut, Philippe; Winch, Christopher

    2012-01-01

    The European Qualification Framework (EQF) is intended to transform European national qualification frameworks (NQFs) by moulding them into a learning outcomes framework. Currently adopted as an enabling law by the European Union, the EQF has now operated for several years. In order to secure widespread adoption, however, it will be necessary for…

  7. 49 CFR 391.55 - LCV Driver-Instructor qualification files.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Section 391.55 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR CARRIER SAFETY REGULATIONS... Driver-Instructor qualification files. (a) Each motor carrier must maintain a qualification file for each...

  8. 29 CFR 1604.2 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 4 2011-07-01 2011-07-01 false Sex as a bona fide occupational qualification. 1604.2... GUIDELINES ON DISCRIMINATION BECAUSE OF SEX § 1604.2 Sex as a bona fide occupational qualification. (a) The commission believes that the bona fide occupational qualification exception as to sex should be interpreted...

  9. 29 CFR 1604.2 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 4 2012-07-01 2012-07-01 false Sex as a bona fide occupational qualification. 1604.2... GUIDELINES ON DISCRIMINATION BECAUSE OF SEX § 1604.2 Sex as a bona fide occupational qualification. (a) The commission believes that the bona fide occupational qualification exception as to sex should be interpreted...

  10. 29 CFR 1604.2 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 4 2013-07-01 2013-07-01 false Sex as a bona fide occupational qualification. 1604.2... GUIDELINES ON DISCRIMINATION BECAUSE OF SEX § 1604.2 Sex as a bona fide occupational qualification. (a) The commission believes that the bona fide occupational qualification exception as to sex should be interpreted...

  11. 29 CFR 1604.2 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 4 2014-07-01 2014-07-01 false Sex as a bona fide occupational qualification. 1604.2... GUIDELINES ON DISCRIMINATION BECAUSE OF SEX § 1604.2 Sex as a bona fide occupational qualification. (a) The commission believes that the bona fide occupational qualification exception as to sex should be interpreted...

  12. 29 CFR 1604.2 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Sex as a bona fide occupational qualification. 1604.2... GUIDELINES ON DISCRIMINATION BECAUSE OF SEX § 1604.2 Sex as a bona fide occupational qualification. (a) The commission believes that the bona fide occupational qualification exception as to sex should be interpreted...

  13. A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels.

    PubMed

    Brzica, Hrvoje; Abdullahi, Wazir; Reilly, Bianca G; Ronaldson, Patrick T

    2018-05-07

    The blood-brain barrier (BBB) is a dynamic barrier tissue that responds to various pathophysiological and pharmacological stimuli. Such changes resulting from these stimuli can greatly modulate drug delivery to the brain and, by extension, cause considerable challenges in the treatment of central nervous system (CNS) diseases. Many BBB changes that affect pharmacotherapy, involve proteins that are localized and expressed at the level of endothelial cells. Indeed, such knowledge on BBB physiology in health and disease has sparked considerable interest in the study of these membrane proteins. From a basic science research standpoint, this implies a requirement for a simple but robust and reproducible method for isolation of microvessels from brain tissue harvested from experimental animals. In order to prepare membrane samples from freshly isolated microvessels, it is essential that sample preparations be enriched in endothelial cells but limited in the presence of other cell types of the neurovascular unit (i.e., astrocytes, microglia, neurons, pericytes). An added benefit is the ability to prepare samples from individual animals in order to capture the true variability of protein expression in an experimental population. In this manuscript, details regarding a method that is utilized for isolation of rat brain microvessels and preparation of membrane samples are provided. Microvessel enrichment, from samples derived, is achieved by using four centrifugation steps where dextran is included in the sample buffer. This protocol can easily be adapted by other laboratories for their own specific applications. Samples generated from this protocol have been shown to yield robust experimental data from protein analysis experiments that can greatly aid the understanding of BBB responses to physiological, pathophysiological, and pharmacological stimuli.

  14. 14 CFR 125.281 - Pilot-in-command qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Pilot-in-command qualifications. 125.281... Requirements § 125.281 Pilot-in-command qualifications. No certificate holder may use any person, nor may any person serve, as pilot in command of an airplane unless that person— (a) Holds at least a commercial...

  15. 14 CFR 125.283 - Second-in-command qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Second-in-command qualifications. 125.283... Requirements § 125.283 Second-in-command qualifications. No certificate holder may use any person, nor may any person serve, as second in command of an airplane unless that person— (a) Holds at least a commercial...

  16. 14 CFR 125.283 - Second-in-command qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Second-in-command qualifications. 125.283... Requirements § 125.283 Second-in-command qualifications. No certificate holder may use any person, nor may any person serve, as second in command of an airplane unless that person— (a) Holds at least a commercial...

  17. 14 CFR 125.281 - Pilot-in-command qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Pilot-in-command qualifications. 125.281... Requirements § 125.281 Pilot-in-command qualifications. No certificate holder may use any person, nor may any person serve, as pilot in command of an airplane unless that person— (a) Holds at least a commercial...

  18. 14 CFR 125.283 - Second-in-command qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Second-in-command qualifications. 125.283... Requirements § 125.283 Second-in-command qualifications. No certificate holder may use any person, nor may any person serve, as second in command of an airplane unless that person— (a) Holds at least a commercial...

  19. 14 CFR 125.283 - Second-in-command qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Second-in-command qualifications. 125.283... Requirements § 125.283 Second-in-command qualifications. No certificate holder may use any person, nor may any person serve, as second in command of an airplane unless that person— (a) Holds at least a commercial...

  20. 14 CFR 125.283 - Second-in-command qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Second-in-command qualifications. 125.283... Requirements § 125.283 Second-in-command qualifications. No certificate holder may use any person, nor may any person serve, as second in command of an airplane unless that person— (a) Holds at least a commercial...

  1. 14 CFR 125.281 - Pilot-in-command qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Pilot-in-command qualifications. 125.281... Requirements § 125.281 Pilot-in-command qualifications. No certificate holder may use any person, nor may any person serve, as pilot in command of an airplane unless that person— (a) Holds at least a commercial...

  2. 14 CFR 125.281 - Pilot-in-command qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Pilot-in-command qualifications. 125.281... Requirements § 125.281 Pilot-in-command qualifications. No certificate holder may use any person, nor may any person serve, as pilot in command of an airplane unless that person— (a) Holds at least a commercial...

  3. 14 CFR 125.281 - Pilot-in-command qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Pilot-in-command qualifications. 125.281... Requirements § 125.281 Pilot-in-command qualifications. No certificate holder may use any person, nor may any person serve, as pilot in command of an airplane unless that person— (a) Holds at least a commercial...

  4. 29 CFR 1910.410 - Qualifications of dive team.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 5 2013-07-01 2013-07-01 false Qualifications of dive team. 1910.410 Section 1910.410... Requirements § 1910.410 Qualifications of dive team. (a) General. (1) Each dive team member shall have the experience or training necessary to perform assigned tasks in a safe and healthful manner. (2) Each dive team...

  5. 29 CFR 1910.410 - Qualifications of dive team.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 5 2014-07-01 2014-07-01 false Qualifications of dive team. 1910.410 Section 1910.410... Requirements § 1910.410 Qualifications of dive team. (a) General. (1) Each dive team member shall have the experience or training necessary to perform assigned tasks in a safe and healthful manner. (2) Each dive team...

  6. 29 CFR 1910.410 - Qualifications of dive team.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 5 2011-07-01 2011-07-01 false Qualifications of dive team. 1910.410 Section 1910.410... Requirements § 1910.410 Qualifications of dive team. (a) General. (1) Each dive team member shall have the experience or training necessary to perform assigned tasks in a safe and healthful manner. (2) Each dive team...

  7. 29 CFR 1910.410 - Qualifications of dive team.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 5 2012-07-01 2012-07-01 false Qualifications of dive team. 1910.410 Section 1910.410... Requirements § 1910.410 Qualifications of dive team. (a) General. (1) Each dive team member shall have the experience or training necessary to perform assigned tasks in a safe and healthful manner. (2) Each dive team...

  8. The Impact of Part-Time Jobs in Years 12 and 13 on Qualification Achievement.

    ERIC Educational Resources Information Center

    Payne, Joan

    2003-01-01

    Presents data from nationally representative sample of the England/Wales Youth Cohort Study, describing patterns of paid work among full-time students in Years 12/13 working for qualifications. Shows paid work of a few hours has negligible impact on "A" level grades, but long working hours significantly reduce grades. (BT)

  9. Preparing Monodisperse Macromolecular Samples for Successful Biological Small-Angle X-ray and Neutron Scattering Experiments

    PubMed Central

    Jeffries, Cy M.; Graewert, Melissa A.; Blanchet, Clément E.; Langley, David B.; Whitten, Andrew E.; Svergun, Dmitri I

    2017-01-01

    Small-angle X-ray and neutron scattering (SAXS and SANS) are techniques used to extract structural parameters and determine the overall structures and shapes of biological macromolecules, complexes and assemblies in solution. The scattering intensities measured from a sample contain contributions from all atoms within the illuminated sample volume including the solvent and buffer components as well as the macromolecules of interest. In order to obtain structural information, it is essential to prepare an exactly matched solvent blank so that background scattering contributions can be accurately subtracted from the sample scattering to obtain the net scattering from the macromolecules in the sample. In addition, sample heterogeneity caused by contaminants, aggregates, mismatched solvents, radiation damage or other factors can severely influence and complicate data analysis so it is essential that the samples are pure and monodisperse for the duration of the experiment. This Protocol outlines the basic physics of SAXS and SANS and reveals how the underlying conceptual principles of the techniques ultimately ‘translate’ into practical laboratory guidance for the production of samples of sufficiently high quality for scattering experiments. The procedure describes how to prepare and characterize protein and nucleic acid samples for both SAXS and SANS using gel electrophoresis, size exclusion chromatography and light scattering. Also included are procedures specific to X-rays (in-line size exclusion chromatography SAXS) and neutrons, specifically preparing samples for contrast matching/variation experiments and deuterium labeling of proteins. PMID:27711050

  10. Saccharose solid matrix embedded proteins: a new method for sample preparation for X-ray absorption spectroscopy.

    PubMed

    Ascone, I; Sabatucci, A; Bubacco, L; Di Muro, P; Salvato, B

    2000-01-01

    In this study, solid samples of hemoglobin and hemocyanin have been prepared by embedding the proteins into a saccharose-based matrix. These materials have been developed specifically for specimens for X-ray absorption spectroscopy (XAS). The preservation of protein conformation and active site organization was tested, making comparisons between the solid and the corresponding liquid samples, using resonance Raman, infra red, fluorescence and XAS. The XAS spectra of irradiated solid and liquid samples were then compared, and the preservation of biological activity of the proteins during both preparation procedure and X-ray irradiation was assessed. In all cases, the measurements clearly demonstrate that protein solid samples are both structurally and functionally quite well preserved, much better than those in the liquid state. The saccharose matrix provides an excellent protection against X-ray damages, allowing for longer exposure to the X-ray beam. Moreover, the demonstrated long-term stability of samples permits their preparation and storage in optimal conditions, allowing for the repetition of data collection with the same sample in several experimental sessions. The very high protein concentration that can be reached results in a significantly better signal-to-noise ratio, particularly useful for high molecular weight proteins with a low metal-to-protein ratio. On the bases of the above-mentioned results, we propose the new method as a standard procedure for the preparation of biological samples to be used for XAS spectroscopy.

  11. Qualification Lab Testing on M1 Abrams Engine Oil Filters

    DTIC Science & Technology

    2016-11-01

    UNCLASSIFIED QUALIFICATION LAB TESTING ON M1 ABRAMS ENGINE OIL FILTERS FINAL REPORT TFLRF No. 483 by Kristi K. Rutta U.S...the originator. UNCLASSIFIED QUALIFICATION LAB TESTING ON M1 ABRAMS ENGINE OIL FILTERS FINAL REPORT TFLRF No. 483 by Kristi K...TITLE AND SUBTITLE Qualification Lab Testing on M1 Abrams Engine Oil Filter 5a. CONTRACT NUMBER W56HZV-15-C-0030 5b. GRANT NUMBER 5c. PROGRAM

  12. Qualifications of Subject Teachers in Special Education Schools

    ERIC Educational Resources Information Center

    Rasmussen, Meryem Uçar; Kis, Arzu

    2018-01-01

    Teacher qualifications are essential to be able to teach children with special needs efficiently. Therefore the aim of this study is to determine the qualifications of subject teachers in special education schools in Turkey. In the study 20 subject teachers within the field of music, art and sports who worked in special education schools in Turkey…

  13. 75 FR 4622 - Qualification of Drivers; Exemption Applications; Diabetes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-28

    ... diabetes-related complications. Each meets the vision standard at 49 CFR 391.41(b)(10). The qualifications... hypoglycemia, significant complications, or inability to manage diabetes; also, any involvement in an accident...-2009-0290] Qualification of Drivers; Exemption Applications; Diabetes AGENCY: Federal Motor Carrier...

  14. An overview of the main foodstuff sample preparation technologies for tetracycline residue determination.

    PubMed

    Pérez-Rodríguez, Michael; Pellerano, Roberto Gerardo; Pezza, Leonardo; Pezza, Helena Redigolo

    2018-05-15

    Tetracyclines are widely used for both the treatment and prevention of diseases in animals as well as for the promotion of rapid animal growth and weight gain. This practice may result in trace amounts of these drugs in products of animal origin, such as milk and eggs, posing serious risks to human health. The presence of tetracycline residues in foods can lead to the transmission of antibiotic-resistant pathogenic bacteria through the food chain. In order to ensure food safety and avoid exposure to these substances, national and international regulatory agencies have established tolerance levels for authorized veterinary drugs, including tetracycline antimicrobials. In view of that, numerous sensitive and specific methods have been developed for the quantification of these compounds in different food matrices. One will note, however, that the determination of trace residues in foods such as milk and eggs often requires extensive sample extraction and preparation prior to conducting instrumental analysis. Sample pretreatment is usually the most complicated step in the analytical process and covers both cleaning and pre-concentration. Optimal sample preparation can reduce analysis time and sources of error, enhance sensitivity, apart from enabling unequivocal identification, confirmation and quantification of target analytes. The development and implementation of more environmentally friendly analytical procedures, which involve the use of less hazardous solvents and smaller sample sizes compared to traditional methods, is a rapidly increasing trend in analytical chemistry. This review seeks to provide an updated overview of the main trends in sample preparation for the determination of tetracycline residues in foodstuffs. The applicability of several extraction and clean-up techniques employed in the analysis of foodstuffs, especially milk and egg samples, is also thoroughly discussed. Copyright © 2018 Elsevier B.V. All rights reserved.

  15. 42 CFR 21.47 - Examination; anticipation of meeting qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Examination; anticipation of meeting qualifications. 21.47 Section 21.47 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES PERSONNEL COMMISSIONED OFFICERS Appointment § 21.47 Examination; anticipation of meeting qualifications. A...

  16. 39 CFR 501.3 - Postage Evidencing System provider qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MANUFACTURE AND DISTRIBUTE POSTAGE EVIDENCING SYSTEMS § 501.3 Postage Evidencing System provider qualification. Any person or entity seeking authorization to manufacture and/or distribute Postage Evidencing Systems... 39 Postal Service 1 2010-07-01 2010-07-01 false Postage Evidencing System provider qualification...

  17. 39 CFR 501.3 - Postage Evidencing System provider qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 39 Postal Service 1 2013-07-01 2013-07-01 false Postage Evidencing System provider qualification... MANUFACTURE AND DISTRIBUTE POSTAGE EVIDENCING SYSTEMS § 501.3 Postage Evidencing System provider qualification. Any person or entity seeking authorization to manufacture and/or distribute Postage Evidencing Systems...

  18. 39 CFR 501.3 - Postage Evidencing System provider qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., distribution, and maintenance of Postage Evidencing Systems and their replacement or secure disposal or... 39 Postal Service 1 2011-07-01 2011-07-01 false Postage Evidencing System provider qualification... MANUFACTURE AND DISTRIBUTE POSTAGE EVIDENCING SYSTEMS § 501.3 Postage Evidencing System provider qualification...

  19. Staubli TX-90XL robot qualification at the LLIHE.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Covert, Timothy Todd

    The Light Initiated High Explosive (LIHE) Facility uses a robotic arm to spray explosive material onto test items for impulse tests. In 2007, the decision was made to replace the existing PUMA 760 robot with the Staubli TX-90XL. A qualification plan was developed and implemented to verify the safe operating conditions and failure modes of the new system. The robot satisfied the safety requirements established in the qualification plan. A performance issue described in this report remains unresolved at the time of this publication. The final readiness review concluded the qualification of this robot at the LIHE facility.

  20. M3FT-17OR0301070211 - Preparation of Hot Isostatically Pressed AgZ Waste Form Samples

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jubin, Robert Thomas; Bruffey, Stephanie H.; Jordan, Jacob A.

    The production of radioactive iodine-bearing waste forms that exhibit long-term stability and are suitable for permanent geologic disposal has been the subject of substantial research interest. One potential method of iodine waste form production is hot isostatic pressing (HIP). Recent studies at Oak Ridge National Laboratory (ORNL) have investigated the conversion of iodine-loaded silver mordenite (I-AgZ) directly to a waste form by HIP. ORNL has performed HIP with a variety of sample compositions and pressing conditions. The base mineral has varied among AgZ (in pure and engineered forms), silver-exchanged faujasite, and silverexchanged zeolite A. Two iodine loading methods, occlusion andmore » chemisorption, have been explored. Additionally, the effects of variations in temperature and pressure of the process have been examined, with temperature ranges of 525°C–1,100°C and pressure ranges of 100–300 MPa. All of these samples remain available to collaborators upon request. The sample preparation detailed in this document is an extension of that work. In addition to previously prepared samples, this report documents the preparation of additional samples to support stability testing. These samples include chemisorbed I-AgZ and pure AgI. Following sample preparation, each sample was processed by HIP by American Isostatic Presses Inc. and returned to ORNL for storage. ORNL will store the samples until they are requested by collaborators for durability testing. The sample set reported here will support waste form durability testing across the national laboratories and will provide insight into the effects of varied iodine content on iodine retention by the produced waste form and on potential improvements in waste form durability provided by the zeolite matrix.« less

  1. SULTAN measurement and qualification: ITER-US-LLNL-NMARTOVETSKY- 092008

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Martovetsky, N N

    2006-09-21

    Measuring the characteristics of full scale ITER CICC at SULTAN is the critical qualification test. If volt-ampere characteristic (VAC) or volt-temperature characteristic (VTC) are distorted, the criterion of 10 uV/m may not be a valid criterion to judge the conductor performance. Only measurements with a clear absence or low signals from the current distribution should be considered as quantitatively representative, although in some obvious circumstances one can judge if a conductor will meet or fail ITER requirements. SULTAN full scale ITER CICC testing should be done with all measures taken to ensure uniform current redistribution. A full removal of Crmore » plating in the joint area and complete solder filling of the joints (with provision of the central channel for helium flow) should be mandatory for DC qualification samples for ITER. Also, T and I should be increased slowly that an equilibrium could be established for accurate measurement of Tcs, Ic and N. It is also desirable to go up in down in current and/or temperature (within stable range) to make sure that the equilibrium is reached.« less

  2. Challenges of biological sample preparation for SIMS imaging of elements and molecules at subcellular resolution

    NASA Astrophysics Data System (ADS)

    Chandra, Subhash

    2008-12-01

    Secondary ion mass spectrometry (SIMS) based imaging techniques capable of subcellular resolution characterization of elements and molecules are becoming valuable tools in many areas of biology and medicine. Due to high vacuum requirements of SIMS, the live cells cannot be analyzed directly in the instrument. The sample preparation, therefore, plays a critical role in preserving the native chemical composition for SIMS analysis. This work focuses on the evaluation of frozen-hydrated and frozen freeze-dried sample preparations for SIMS studies of cultured cells with a CAMECA IMS-3f dynamic SIMS ion microscope instrument capable of producing SIMS images with a spatial resolution of 500 nm. The sandwich freeze-fracture method was used for fracturing the cells. The complimentary fracture planes in the plasma membrane were characterized by field-emission secondary electron microscopy (FESEM) in the frozen-hydrated state. The cells fractured at the dorsal surface were used for SIMS analysis. The frozen-hydrated SIMS analysis of individual cells under dynamic primary ion beam (O 2+) revealed local secondary ion signal enhancements correlated with the water image signals of 19(H 3O) +. A preferential removal of water from the frozen cell matrix in the Z-axis was also observed. These complications render the frozen-hydrated sample type less desirable for subcellular dynamic SIMS studies. The freeze-drying of frozen-hydrated cells, either inside the instrument or externally in a freeze-drier, allowed SIMS imaging of subcellular chemical composition. Morphological evaluations of fractured freeze-dried cells with SEM and confocal laser scanning microscopy (CLSM) revealed well-preserved mitochondria, Golgi apparatus, and stress fibers. SIMS analysis of fractured freeze-dried cells revealed well-preserved chemical composition of even the most highly diffusible ions like K + and Na + in physiologically relevant concentrations. The high K-low Na signature in individual cells

  3. 14C sample preparation for AMS microdosing studies at Lund University using online combustion and septa-sealed vials

    NASA Astrophysics Data System (ADS)

    Sydoff, Marie; Stenström, Kristina

    2010-04-01

    The Department of Physics at Lund University is participating in a European Union project called EUMAPP (European Union Microdose AMS Partnership Programme), in which sample preparation and accelerator mass spectrometry (AMS) measurements of biological samples from microdosing studies have been made. This paper describes a simplified method of converting biological samples to solid graphite for 14C analysis with AMS. The method is based on online combustion of the samples, and reduction of CO 2 in septa-sealed vials. The septa-sealed vials and disposable materials are used to eliminate sample cross-contamination. Measurements of ANU and Ox I standards show deviations of 2% and 3%, respectively, relative to reference values. This level of accuracy is sufficient for biological samples from microdosing studies. Since the method has very few handling steps from sample to graphite, the risk of failure during the sample preparation process is minimized, making the method easy to use in routine preparation of samples.

  4. 10 CFR 1046.15 - Training and qualification for security skills and knowledge.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Training and qualification for security skills and... SECURITY INTERESTS Protective Force Personnel § 1046.15 Training and qualification for security skills and... and Qualification for Security Skills and Knowledge,” to this subpart. The DOE contractor shall...

  5. Rapid Microbial Sample Preparation from Blood Using a Novel Concentration Device

    PubMed Central

    Boardman, Anna K.; Campbell, Jennifer; Wirz, Holger; Sharon, Andre; Sauer-Budge, Alexis F.

    2015-01-01

    Appropriate care for bacteremic patients is dictated by the amount of time needed for an accurate diagnosis. However, the concentration of microbes in the blood is extremely low in these patients (1–100 CFU/mL), traditionally requiring growth (blood culture) or amplification (e.g., PCR) for detection. Current culture-based methods can take a minimum of two days, while faster methods like PCR require a sample free of inhibitors (i.e., blood components). Though commercial kits exist for the removal of blood from these samples, they typically capture only DNA, thereby necessitating the use of blood culture for antimicrobial testing. Here, we report a novel, scaled-up sample preparation protocol carried out in a new microbial concentration device. The process can efficiently lyse 10 mL of bacteremic blood while maintaining the microorganisms’ viability, giving a 30‑μL final output volume. A suite of six microorganisms (Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Haemophilus influenzae, Pseudomonas aeruginosa, and Candida albicans) at a range of clinically relevant concentrations was tested. All of the microorganisms had recoveries greater than 55% at the highest tested concentration of 100 CFU/mL, with three of them having over 70% recovery. At the lowest tested concentration of 3 CFU/mL, two microorganisms had recoveries of ca. 40–50% while the other four gave recoveries greater than 70%. Using a Taqman assay for methicillin-sensitive S. aureus (MSSA)to prove the feasibility of downstream analysis, we show that our microbial pellets are clean enough for PCR amplification. PCR testing of 56 spiked-positive and negative samples gave a specificity of 0.97 and a sensitivity of 0.96, showing that our sample preparation protocol holds great promise for the rapid diagnosis of bacteremia directly from a primary sample. PMID:25675242

  6. Reducing Time and Increasing Sensitivity in Sample Preparation for Adherent Mammalian Cell Metabolomics

    PubMed Central

    Lorenz, Matthew A.; Burant, Charles F.; Kennedy, Robert T.

    2011-01-01

    A simple, fast, and reproducible sample preparation procedure was developed for relative quantification of metabolites in adherent mammalian cells using the clonal β-cell line INS-1 as a model sample. The method was developed by evaluating the effect of different sample preparation procedures on high performance liquid chromatography- mass spectrometry quantification of 27 metabolites involved in glycolysis and the tricarboxylic acid cycle on a directed basis as well as for all detectable chromatographic features on an undirected basis. We demonstrate that a rapid water rinse step prior to quenching of metabolism reduces components that suppress electrospray ionization thereby increasing signal for 26 of 27 targeted metabolites and increasing total number of detected features from 237 to 452 with no detectable change of metabolite content. A novel quenching technique is employed which involves addition of liquid nitrogen directly to the culture dish and allows for samples to be stored at −80 °C for at least 7 d before extraction. Separation of quenching and extraction steps provides the benefit of increased experimental convenience and sample stability while maintaining metabolite content similar to techniques that employ simultaneous quenching and extraction with cold organic solvent. The extraction solvent 9:1 methanol: chloroform was found to provide superior performance over acetonitrile, ethanol, and methanol with respect to metabolite recovery and extract stability. Maximal recovery was achieved using a single rapid (~1 min) extraction step. The utility of this rapid preparation method (~5 min) was demonstrated through precise metabolite measurements (11% average relative standard deviation without internal standards) associated with step changes in glucose concentration that evoke insulin secretion in the clonal β-cell line INS-1. PMID:21456517

  7. The role of sample preparation in interpretation of trace element concentration variability in moss bioindication studies

    USGS Publications Warehouse

    Migaszewski, Z.M.; Lamothe, P.J.; Crock, J.G.; Galuszka, A.; Dolegowska, S.

    2011-01-01

    Trace element concentrations in plant bioindicators are often determined to assess the quality of the environment. Instrumental methods used for trace element determination require digestion of samples. There are different methods of sample preparation for trace element analysis, and the selection of the best method should be fitted for the purpose of a study. Our hypothesis is that the method of sample preparation is important for interpretation of the results. Here we compare the results of 36 element determinations performed by ICP-MS on ashed and on acid-digested (HNO3, H2O2) samples of two moss species (Hylocomium splendens and Pleurozium schreberi) collected in Alaska and in south-central Poland. We found that dry ashing of the moss samples prior to analysis resulted in considerably lower detection limits of all the elements examined. We also show that this sample preparation technique facilitated the determination of interregional and interspecies differences in the chemistry of trace elements. Compared to the Polish mosses, the Alaskan mosses displayed more positive correlations of the major rock-forming elements with ash content, reflecting those elements' geogenic origin. Of the two moss species, P. schreberi from both Alaska and Poland was also highlighted by a larger number of positive element pair correlations. The cluster analysis suggests that the more uniform element distribution pattern of the Polish mosses primarily reflects regional air pollution sources. Our study has shown that the method of sample preparation is an important factor in statistical interpretation of the results of trace element determinations. ?? 2010 Springer-Verlag.

  8. Qualifications Frameworks and Their Conflicting Social Imaginaries of Globalisation

    ERIC Educational Resources Information Center

    Louise Sarauw, Laura

    2012-01-01

    Critics often see the European Bologna Process as a univocal standardisation of higher education. By exploring how different qualifications frameworks project different social imaginaries of globalisation, this article takes a different stance. The overarching qualifications framework of the Bologna Process rests on a socially constituted and…

  9. 48 CFR 1252.237-70 - Qualifications of contractor employees.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... contractor employees. 1252.237-70 Section 1252.237-70 Federal Acquisition Regulations System DEPARTMENT OF....237-70 Qualifications of contractor employees. As prescribed in (TAR) 48 CFR 1237.110(a), insert the following clause: Qualifications of Contractor Employees (APR 2005) a. Definitions. As used in this clause...

  10. Options and Risk for Qualification of Electric Propulsion System

    NASA Technical Reports Server (NTRS)

    Bailey, Michelle; Daniel, Charles; Cook, Steve (Technical Monitor)

    2002-01-01

    Electric propulsion vehicle systems envelop a wide range of propulsion alternatives including solar and nuclear, which present unique circumstances for qualification. This paper will address the alternatives for qualification of electric propulsion spacecraft systems. The approach taken will be to address the considerations for qualification at the various levels of systems definition. Additionally, for each level of qualification the system level risk implications will be developed. Also, the paper will explore the implications of analysis verses test for various levels of systems definition, while retaining the objectives of a verification program. The limitations of terrestrial testing will be explored along with the risk and implications of orbital demonstration testing. The paper will seek to develop a template for structuring of a verification program based on cost, risk and value return. A successful verification program should establish controls and define objectives of the verification compliance program. Finally the paper will seek to address the political and programmatic factors, which may impact options for system verification.

  11. M-52 spray booth qualification test

    NASA Technical Reports Server (NTRS)

    1990-01-01

    The procedures, performance, and results obtained from the M-52 spray booth qualification test are documented. The test was conducted at Thiokol Corporation, Space Operations, M-52 Inert Parts Preparation facility. The purpose of this testing sequence was to ensure the spray booth would produce flight qualified hardware. The testing sequence was conducted in two series. The first series was conducted under CTP-0142, Revision 1. The second series was conducted in accordance with CTP-0142, Revision 2. The test sequence started with CTP-0142, Revision 1. The series consisted of the contamination removal test and the performance test. The contamination removal test was used to assess the Teflon level in the spray booth. The performance test consisted of painting and Chemloking a forward dome inside the spray booth per flight procedures. During the performance test, two sets of witness panels (case/insulation and steel/epoxy/steel) were prepared and pull tested. The CTP-0142, Revision 2, series of testing consisted of re-testing the steel/epoxy/steel witness panels. The pull tests analysis indicates the results of the tensile tests were comparable to the systems tunnel witness panel database. The exposed panel set and the control panel set average tensile values were above the 1-basis lower limits established on the systems tunnel witness panel database. It is recommended that the M-52 spray booth be qualified for producing flight hardware.

  12. Development of a Novel Self-Enclosed Sample Preparation Device for DNA/RNA Isolation in Space

    NASA Technical Reports Server (NTRS)

    Zhang, Ye; Mehta, Satish K.; Pensinger, Stuart J.; Pickering, Karen D.

    2011-01-01

    Modern biology techniques present potentials for a wide range of molecular, cellular, and biochemistry applications in space, including detection of infectious pathogens and environmental contaminations, monitoring of drug-resistant microbial and dangerous mutations, identification of new phenotypes of microbial and new life species. However, one of the major technological blockades in enabling these technologies in space is a lack of devices for sample preparation in the space environment. To overcome such an obstacle, we constructed a prototype of a DNA/RNA isolation device based on our novel designs documented in the NASA New Technology Reporting System (MSC-24811-1/3-1). This device is self-enclosed and pipette free, purposely designed for use in the absence of gravity. Our design can also be modified easily for preparing samples in space for other applications, such as flowcytometry, immunostaining, cell separation, sample purification and separation according to its size and charges, sample chemical labeling, and sample purification. The prototype of our DNA/RNA isolation device was tested for efficiencies of DNA and RNA isolation from various cell types for PCR analysis. The purity and integrity of purified DNA and RNA were determined as well. Results showed that our developed DNA/RNA isolation device offers similar efficiency and quality in comparison to the samples prepared using the standard protocol in the laboratory.

  13. Soil and Water – What is Detectable through Microbiological Sample Preparation Techniques

    EPA Science Inventory

    The concerns of a potential terrorist’s use of biological agents in soil and ground water are articulated by comparisons to major illnesses in this Country involving contaminated drinking water sources. Objectives are focused on the importance of sample preparation in the rapid, ...

  14. Inspection qualification (IQ) and operational qualification (OQ) for a vacuum freeze-dryer--Part II: Inspection qualification.

    PubMed

    Jennings, T A; Scheer, A; Emodi, A; Puderbach, L; King, S; Norton, T

    1996-01-01

    This paper demonstrates the importance of the inspection qualification (IQ). It was shown that the facilities were adequate to house the dryer and its associated instrumentation, and that the facilities had sufficient utilities to operate the dryer. The IQ established that the design and construction of the dryer was in agreement with the proposed hardware and design specifications. It was verified that the dryer was constructed in accordance with specifications set-forth by the equipment manufacturer.

  15. Flight qualification of mortar-actuated parachute deployment systems

    NASA Technical Reports Server (NTRS)

    Pleasants, J. E.

    1975-01-01

    A brief discussion outlines background of mortar use in parachute deployment systems. A description of the system operation is presented. Effects of the environment on performance are discussed as well as the instrumentation needed to assess this performance. Power unit qualification and lot qualification for shear pins and cartridges is delineated. Functional mortar system tests are described. Finally, bridle deployment and parachute deployment are discussed.

  16. Sample Preparation Report of the Fourth OPCW Confidence Building Exercise on Biomedical Sample Analysis

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Udey, R. N.; Corzett, T. H.; Alcaraz, A.

    Following the successful completion of the 3rd biomedical confidence building exercise (February 2013 – March 2013), which included the analysis of plasma and urine samples spiked at low ppb levels as part of the exercise scenario, another confidence building exercise was targeted to be conducted in 2014. In this 4th exercise, it was desired to focus specifically on the analysis of plasma samples. The scenario was designed as an investigation of an alleged use of chemical weapons where plasma samples were collected, as plasma has been reported to contain CWA adducts which remain present in the human body for severalmore » weeks (Solano et al. 2008). In the 3rd exercise most participants used the fluoride regeneration method to analyze for the presence of nerve agents in plasma samples. For the 4th biomedical exercise it was decided to evaluate the analysis of human plasma samples for the presence/absence of the VX adducts and aged adducts to blood proteins (e.g., VX-butyrylcholinesterase (BuChE) and aged BuChE adducts using a pepsin digest technique to yield nonapeptides; or equivalent). As the aging of VX-BuChE adducts is relatively slow (t1/2 = 77 hr at 37 °C [Aurbek et al. 2009]), soman (GD), which ages much more quickly (t1/2 = 9 min at 37 °C [Masson et al. 2010]), was used to simulate an aged VX sample. Additional objectives of this exercise included having laboratories assess novel OP-adducted plasma sample preparation techniques and analytical instrumentation methodologies, as well as refining/designating the reporting formats for these new techniques.« less

  17. 12 CFR 231.3 - Qualification as a financial institution.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Qualification as a financial institution. 231.3... RESERVE SYSTEM NETTING ELIGIBILITY FOR FINANCIAL INSTITUTION (REGULATION EE) § 231.3 Qualification as a financial institution. (a) A person qualifies as a financial institution for purposes of sections 401-407 of...

  18. Training and Qualifications (Teachers and Workers for the Deaf).

    ERIC Educational Resources Information Center

    World Federation of the Deaf, Rome (Italy).

    Three papers consider the training and qualifications of teachers and workers for the deaf. H. Okopinski describes "Training Teachers for Deaf Children's Schools in Poland" and A. F. Mackenzie defines "The Qualifications of Workers for the Adult Deaf" in the United Kingdom. E. S. Levine reports on New York University's…

  19. 27 CFR 41.114a - Qualification for extended deferral.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2014-04-01 2014-04-01 false Qualification for extended... Qualification for extended deferral. Note: This section applies only to removals made before January 1, 1983. (a... of the extended deferral permitted by § 41.114 shall file with the appropriate TTB officer an...

  20. 27 CFR 41.114a - Qualification for extended deferral.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2013-04-01 2013-04-01 false Qualification for extended... Qualification for extended deferral. Note: This section applies only to removals made before January 1, 1983. (a... of the extended deferral permitted by § 41.114 shall file with the appropriate TTB officer an...

  1. 27 CFR 41.114a - Qualification for extended deferral.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2012-04-01 2011-04-01 true Qualification for extended... Qualification for extended deferral. Note: This section applies only to removals made before January 1, 1983. (a... of the extended deferral permitted by § 41.114 shall file with the appropriate TTB officer an...

  2. Difficulties in Recognising Vocational Skills and Qualifications across Europe

    ERIC Educational Resources Information Center

    Brockmann, Michaela; Clarke, Linda; Winch, Christopher

    2009-01-01

    The development of the European Qualification Framework (EQF) as a means of assessing the relative status of qualifications across the EU is described, together with its fundamental design characteristics, particularly the adoption of a "learning outcomes" approach. Ways in which it may be implemented, and possible difficulties in this,…

  3. 24 CFR 20.3 - Location, organization, and officer qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... speech-impaired individuals may access this number via TTY by calling the toll-free Federal Information... qualifications. 20.3 Section 20.3 Housing and Urban Development Office of the Secretary, Department of Housing... qualifications. (a) Mailing address. The Office of Hearings and Appeals is located at the U.S. Department of...

  4. Cytology Preparations of Formalin Fixative Aid Detection of Giardia in Duodenal Biopsy Samples.

    PubMed

    Panarelli, Nicole C; Gobara, Nariman; Hoda, Rana S; Chaump, Michael; Jessurun, Jose; Yantiss, Rhonda K

    2017-04-01

    Giardiasis is the most common intestinal parasitic infection in the United States. The organism elicits no, or minimal, inflammatory changes in duodenal biopsy samples, so it can be easily overlooked. We performed this study to determine whether Giardia could be isolated from the formalin fixative of biopsy samples, and to evaluate the value of fluid analysis in the assessment for potential infection. We prospectively evaluated duodenal biopsy samples from 92 patients with a clinical suspicion of giardiasis or symptoms compatible with that diagnosis (ie, diarrhea, bloating, or abdominal pain) Biopsy samples were routinely processed and stained with hematoxylin and eosin. Histologic diagnoses included giardiasis (5 cases, 4%), normal findings (64 cases, 70%), peptic injury/active duodenitis (12 cases, 13%), and intraepithelial lymphocytosis with villous blunting (10 cases, 12%). Fifteen cases (13%) showed detached degenerated epithelial cells or mucus droplets in the intervillous space that resembled Giardia. Cytology slides were prepared from formalin in the biopsy container using the standard Cytospin protocol and reviewed by a cytopathologist blinded to the biopsy findings. Cytologic evaluation revealed Giardia spp. in all 5 biopsy-proven cases, and identified an additional case that was not detected by biopsy analysis. Organisms were significantly more numerous (mean: 400 trophozoites; range, 120 to 810) and showed better morphologic features in cytology preparations compared with tissue sections (mean: 129 trophozoites; range, 37 to 253 organisms; P=0.05). Our findings suggest that cytology preparations from formalin fixative can resolve diagnostically challenging cases and even enhance Giardia detection in some cases.

  5. Methods of biological fluids sample preparation - biogenic amines, methylxanthines, water-soluble vitamins.

    PubMed

    Płonka, Joanna

    2015-01-01

    In recent years demands on the amount of information that can be obtained from the analysis of a single sample have increased. For time and economic reasons it is necessary to examine at the same time larger number of compounds, and compounds from different groups. This can best be seen in such areas as clinical analysis. In many diseases, the best results for patients are obtained when treatment fits the individual characteristics of the patient. Dosage monitoring is important at the beginning of therapy and in the full process of treatment. In the treatment of many diseases biogenic amines (dopamine, serotonin) and methylxanthines (theophylline, theobromine, caffeine) play an important role. They are used as drugs separately or in combination with others to support and strengthen the action of other drugs - for example, the combination of caffeine and paracetamol. Vitamin supplementation may be also an integral part of the treatment process. Specification of complete sample preparation parameters for extraction of the above compounds from biological matrices has been reviewed. Particular attention was given to the preparation stage and extraction methods. This review provides universal guidance on establishing a common procedures across laboratories to facilitate the preparation and analysis of all discussed compounds. Copyright © 2014 John Wiley & Sons, Ltd.

  6. Autonomous microfluidic sample preparation system for protein profile-based detection of aerosolized bacterial cells and spores.

    PubMed

    Stachowiak, Jeanne C; Shugard, Erin E; Mosier, Bruce P; Renzi, Ronald F; Caton, Pamela F; Ferko, Scott M; Van de Vreugde, James L; Yee, Daniel D; Haroldsen, Brent L; VanderNoot, Victoria A

    2007-08-01

    For domestic and military security, an autonomous system capable of continuously monitoring for airborne biothreat agents is necessary. At present, no system meets the requirements for size, speed, sensitivity, and selectivity to warn against and lead to the prevention of infection in field settings. We present a fully automated system for the detection of aerosolized bacterial biothreat agents such as Bacillus subtilis (surrogate for Bacillus anthracis) based on protein profiling by chip gel electrophoresis coupled with a microfluidic sample preparation system. Protein profiling has previously been demonstrated to differentiate between bacterial organisms. With the goal of reducing response time, multiple microfluidic component modules, including aerosol collection via a commercially available collector, concentration, thermochemical lysis, size exclusion chromatography, fluorescent labeling, and chip gel electrophoresis were integrated together to create an autonomous collection/sample preparation/analysis system. The cycle time for sample preparation was approximately 5 min, while total cycle time, including chip gel electrophoresis, was approximately 10 min. Sensitivity of the coupled system for the detection of B. subtilis spores was 16 agent-containing particles per liter of air, based on samples that were prepared to simulate those collected by wetted cyclone aerosol collector of approximately 80% efficiency operating for 7 min.

  7. Development of a Preschool Teacher's Guidance Qualifications Scale and Its Psychometric Properties

    ERIC Educational Resources Information Center

    Bilgin, Hülya

    2017-01-01

    The goal of this study is to develop a Guidance Qualification Scale for Preschool Teachers and examine the validity and reliability of the scale. The research was conducted with 374 teachers working in preschools in Istanbul and they were chosen by the random sampling method. Factor analysis was carried out for the construct validity of the scale.…

  8. Overview of Materials Qualification Needs for Metal Additive Manufacturing

    NASA Astrophysics Data System (ADS)

    Seifi, Mohsen; Salem, Ayman; Beuth, Jack; Harrysson, Ola; Lewandowski, John J.

    2016-03-01

    This overview highlights some of the key aspects regarding materials qualification needs across the additive manufacturing (AM) spectrum. AM technology has experienced considerable publicity and growth in the past few years with many successful insertions for non-mission-critical applications. However, to meet the full potential that AM has to offer, especially for flight-critical components (e.g., rotating parts, fracture-critical parts, etc.), qualification and certification efforts are necessary. While development of qualification standards will address some of these needs, this overview outlines some of the other key areas that will need to be considered in the qualification path, including various process-, microstructure-, and fracture-modeling activities in addition to integrating these with lifing activities targeting specific components. Ongoing work in the Advanced Manufacturing and Mechanical Reliability Center at Case Western Reserve University is focusing on fracture and fatigue testing to rapidly assess critical mechanical properties of some titanium alloys before and after post-processing, in addition to conducting nondestructive testing/evaluation using micro-computerized tomography at General Electric. Process mapping studies are being conducted at Carnegie Mellon University while large area microstructure characterization and informatics (EBSD and BSE) analyses are being conducted at Materials Resources LLC to enable future integration of these efforts via an Integrated Computational Materials Engineering approach to AM. Possible future pathways for materials qualification are provided.

  9. Preparing to Receive and Handle Martian Samples When They Arrive on Earth

    NASA Technical Reports Server (NTRS)

    McCubbin, Francis M.

    2017-01-01

    The Astromaterials Acquisition and Curation Office at NASA Johnson Space Center (JSC) is responsible for curating all of NASA's extraterrestrial samples. Under the governing document, NASA Policy Directive (NPD) 7100.10F+ derivative NPR 'Curation of Extraterrestrial Materials', JSC is charged with 'The curation of all extraterrestrial material under NASA control, including future NASA missions. 'The Directive goes on to define Curation as including'...documentation, preservation, preparation, and distribution of samples for research, education, and public outreach."

  10. Recent trends in sorption-based sample preparation and liquid chromatography techniques for food analysis.

    PubMed

    V Soares Maciel, Edvaldo; de Toffoli, Ana Lúcia; Lanças, Fernando Mauro

    2018-04-20

    The accelerated rising of the world's population increased the consumption of food, thus demanding more rigors in the control of residue and contaminants in food-based products marketed for human consumption. In view of the complexity of most food matrices, including fruits, vegetables, different types of meat, beverages, among others, a sample preparation step is important to provide more reliable results when combined with HPLC separations. An adequate sample preparation step before the chromatographic analysis is mandatory in obtaining higher precision and accuracy in order to improve the extraction of the target analytes, one of the priorities in analytical chemistry. The recent discovery of new materials such as ionic liquids, graphene-derived materials, molecularly imprinted polymers, restricted access media, magnetic nanoparticles, and carbonaceous nanomaterials, provided high sensitivity and selectivity results in an extensive variety of applications. These materials, as well as their several possible combinations, have been demonstrated to be highly appropriate for the extraction of different analytes in complex samples such as food products. The main characteristics and application of these new materials in food analysis will be presented and discussed in this paper. Another topic discussed in this review covers the main advantages and limitations of sample preparation microtechniques, as well as their off-line and on-line combination with HPLC for food analysis. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Molecularly imprinted polymers for sample preparation and biosensing in food analysis: Progress and perspectives.

    PubMed

    Ashley, Jon; Shahbazi, Mohammad-Ali; Kant, Krishna; Chidambara, Vinayaka Aaydha; Wolff, Anders; Bang, Dang Duong; Sun, Yi

    2017-05-15

    Molecularly imprinted polymers (MIPs) are biomimetics which can selectively bind to analytes of interest. One of the most interesting areas where MIPs have shown the biggest potential is food analysis. MIPs have found use as sorbents in sample preparation attributed to the high selectivity and high loading capacity. MIPs have been intensively employed in classical solid-phase extraction and solid-phase microextraction. More recently, MIPs have been combined with magnetic bead extraction, which greatly simplifies sample handling procedures. Studies have consistently shown that MIPs can effectively minimize complex food matrix effects, and improve recoveries and detection limits. In addition to sample preparation, MIPs have also been viewed as promising alternatives to bio-receptors due to the inherent molecular recognition abilities and the high stability in harsh chemical and physical conditions. MIPs have been utilized as receptors in biosensing platforms such as electrochemical, optical and mass biosensors to detect various analytes in food. In this review, we will discuss the current state-of-the-art of MIP synthesis and applications in the context of food analysis. We will highlight the imprinting methods which are applicable for imprinting food templates, summarize the recent progress in using MIPs for preparing and analysing food samples, and discuss the current limitations in the commercialisation of MIPs technology. Finally, future perspectives will be given. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. Instructor qualification for radiation safety training at a national laboratory

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Trinoskey, P.A.

    1994-10-01

    Prior to 1993, Health Physics Training (HPT) was conducted by the Lawrence Livermore National Laboratory (LLNL) health physics group. The job requirements specified a Masters Degree and experience. In fact, the majority of Health Physicists in the group were certified by the American Board of Health Physics. Under those circumstances, it was assumed that individuals in the group were technically qualified and the HPT instructor qualification stated that. In late 1993, the Health Physics Group at the LLNL was restructured and the training function was assigned to the training group. Additional requirements for training were mandated by the Department ofmore » Energy (DOE), which would necessitate increasing the existing training staff. With the need to hire, and the policy of reassignment of employees during downsizing, it was imperative that formal qualification standards be developed for technical knowledge. Qualification standards were in place for instructional capability. In drafting the new training qualifications for instructors, the requirements of a Certified Health Physicists had to be modified due to supply and demand. Additionally, for many of the performance-based training courses, registration by the National Registry of Radiation Protection Technologists is more desirable. Flexibility in qualification requirements has been incorporated to meet the reality of ongoing training and the compensation for desirable skills of individuals who may not meet all the criteria. The qualification requirements for an instructor rely on entry-level requirements and emphasis on goals (preferred) and continuing development of technical and instructional capabilities.« less

  13. Data Qualification Report For: Thermodynamic Data File, DATA0.YMP.R0 For Geochemical Code, EQ3/6 

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    P.L. Cloke

    The objective of this work is to evaluate the adequacy of chemical thermodynamic data provided by Lawrence Livermore National Laboratory (LLNL) as DataO.ymp.ROA in response to an input request submitted under AP-3.14Q. This request specified that chemical thermodynamic data available in the file, Data0.com.R2, be updated, improved, and augmented for use in geochemical modeling used in Process Model Reports (PMRs) for Engineered Barrier Systems, Waste Form, Waste Package, Unsaturated Zone, and Near Field Environment, as well as for Performance Assessment. The data are qualified in the temperature range 0 to 100 C. Several Data Tracking Numbers (DTNs) associated with Analysis/Modelmore » Reports (AMR) addressing various aspects of the post-closure chemical behavior of the waste package and the Engineered Barrier System that rely on EQ316 outputs to which these data are used as input, are Principal Factor affecting. This qualification activity was accomplished in accordance with the AP-SIII.2Q using the Technical Assessment method. A development plan, TDP-EBS-MD-000044, was prepared in accordance with AP-2.13Q and approved by the Responsible Manager. In addition, a Process Control Evaluation was performed in accordance with AP-SV.1Q. The qualification method, selected in accordance with AP-SIII.2Q, was Technical Assessment. The rationale for this approach is that the data in File Data0.com.R2 are considered Handbook data and therefore do not themselves require qualification. Only changes to Data0.com.R2 required qualification. A new file has been produced which contains the database Data0.ymp.R0, which is recommended for qualification as a result of this action. Data0.ymp.R0 will supersede Data0.com.R2 for all Yucca Mountain Project (YMP) activities.« less

  14. Automation of preparation of nonmetallic samples for analysis by atomic absorption and inductively coupled plasma spectrometry

    NASA Technical Reports Server (NTRS)

    Wittmann, A.; Willay, G.

    1986-01-01

    For a rapid preparation of solutions intended for analysis by inductively coupled plasma emission spectrometry or atomic absorption spectrometry, an automatic device called Plasmasol was developed. This apparatus used the property of nonwettability of glassy C to fuse the sample in an appropriate flux. The sample-flux mixture is placed in a composite crucible, then heated at high temperature, swirled until full dissolution is achieved, and then poured into a water-filled beaker. After acid addition, dissolution of the melt, and filling to the mark, the solution is ready for analysis. The analytical results obtained, either for oxide samples or for prereduced iron ores show that the solutions prepared with this device are undistinguished from those obtained by manual dissolutions done by acid digestion or by high temperature fusion. Preparation reproducibility and analytical tests illustrate the performance of Plasmasol.

  15. Sport Management Interns--Selection Qualifications.

    ERIC Educational Resources Information Center

    Cuneen, Jacquelyn; Sidwell, M. Joy

    1993-01-01

    Examines and rates the background qualifications and qualities, identified by sport management practitioners through an examination of paper credentials, that are desired in interviewees vying for selection into sport management internship positions. (GLR)

  16. Formal Methods Tool Qualification

    NASA Technical Reports Server (NTRS)

    Wagner, Lucas G.; Cofer, Darren; Slind, Konrad; Tinelli, Cesare; Mebsout, Alain

    2017-01-01

    Formal methods tools have been shown to be effective at finding defects in safety-critical digital systems including avionics systems. The publication of DO-178C and the accompanying formal methods supplement DO-333 allows applicants to obtain certification credit for the use of formal methods without providing justification for them as an alternative method. This project conducted an extensive study of existing formal methods tools, identifying obstacles to their qualification and proposing mitigations for those obstacles. Further, it interprets the qualification guidance for existing formal methods tools and provides case study examples for open source tools. This project also investigates the feasibility of verifying formal methods tools by generating proof certificates which capture proof of the formal methods tool's claim, which can be checked by an independent, proof certificate checking tool. Finally, the project investigates the feasibility of qualifying this proof certificate checker, in the DO-330 framework, in lieu of qualifying the model checker itself.

  17. First experiment on LMJ facility: pointing and synchronisation qualification, sequences qualification

    NASA Astrophysics Data System (ADS)

    Henry, Olivier; Bretheau, Dominique; Luttmann, Michel; Graillot, Herve; Ferri, Michel; Seguineau, Frederic; Bar, Emmanuel; Patissou, Loic; Canal, Phillipe; Sautarel, Françoise; Tranquille Marques, Yves; Raffestin, Didier

    2016-10-01

    The LMJ (Laser mega Joule) facility at the CESTA site (Aquitaine, France) is a tool designed to deliver up to 1.2 MJ at 351 nm for plasma experiments. The experiment system will include 11 diagnostics: UV and X energy balances, imagers (Streak and stripe camera, CCD), spectrometers, and a Visar/pyrometer. The facility must be able to deliver, within the hour following the shot, all the results of the plasma diagnostics, alignment images and laser diagnostic measurements. These results have to be guaranteed in terms of conformity to the request and quality of measurement. The end of 2014 was devoted to the qualification of system pointing on target and synchronization within and between beams. The shots made with one chain (divided in 2 quads - 8 laser beams) have achieved 50 µm of misalignment accuracy (chain and quad channel) and a synchronization accuracy in the order of 50 ps. The performances achieved for plasma diagnostic (in the order of less 100 µm of alignment and timing accuracy less than 150 ps) comply with expectations. At the same time the first automatic sequences were tested. They allowed a shot on target every 6h:30 and in some case twice a day by reducing preparation actions, leading to a sequence of 4h:00. These shooting sequences are managed by an operating team of 7 people helped by 3 people for security aspects.

  18. Qualification Paths of Adult Educators in Sweden and Denmark

    ERIC Educational Resources Information Center

    Andersson, Per; Kopsen, Susanne; Larson, Anne; Milana, Marcella

    2013-01-01

    The qualification of adult educators is a central aspect of the quality of adult education. However, within current policy discourses and adult education research on the professional development of prospective adult educators, little attention is paid to teacher qualification when compared to other fields of education and training. In this study,…

  19. The Growth of Foreign Qualification Suppliers in Sri Lanka: "de facto" Decentralisation?

    ERIC Educational Resources Information Center

    Little, Angela W.; Evans, Jane

    2005-01-01

    Based mainly on a study of newspaper adverts for qualifications and tuition courses in Sri Lanka over a period from 1965 to 2000, this paper describes a decentralisation of control over the supply of qualifications. It is argued that this has occurred not through a deliberate policy mechanism to decentralise qualifications, but rather by default,…

  20. Differentiated Quality Assurance for the African Virtual University's Teacher Education Qualification in Mathematics and Science

    ERIC Educational Resources Information Center

    Hattingh, A.

    2008-01-01

    For the African Virtual University and its consortium of African universities the implementation of quality promoting initiatives are not without challenges and scepticisms. To be discussed in this article is the case of a teacher education qualification in ten different African countries. Seven countries were sampled and visited in 2006 with the…