Actinic imaging and evaluation of phase structures on EUV lithography masks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mochi, Iacopo; Goldberg, Kenneth; Huh, Sungmin

2010-09-28

The authors describe the implementation of a phase-retrieval algorithm to reconstruct phase and complex amplitude of structures on EUV lithography masks. Many native defects commonly found on EUV reticles are difficult to detect and review accurately because they have a strong phase component. Understanding the complex amplitude of mask features is essential for predictive modeling of defect printability and defect repair. Besides printing in a stepper, the most accurate way to characterize such defects is with actinic inspection, performed at the design, EUV wavelength. Phase defect and phase structures show a distinct through-focus behavior that enables qualitative evaluation of themore » object phase from two or more high-resolution intensity measurements. For the first time, phase of structures and defects on EUV masks were quantitatively reconstructed based on aerial image measurements, using a modified version of a phase-retrieval algorithm developed to test optical phase shifting reticles.« less

Non-interferometric phase retrieval using refractive index manipulation

PubMed Central

Chen, Chyong-Hua; Hsu, Hsin-Feng; Chen, Hou-Ren; Hsieh, Wen-Feng

2017-01-01

We present a novel, inexpensive and non-interferometric technique to retrieve phase images by using a liquid crystal phase shifter without including any physically moving parts. First, we derive a new equation of the intensity-phase relation with respect to the change of refractive index, which is similar to the transport of the intensity equation. The equation indicates that this technique is unneeded to consider the variation of magnifications between optical images. For proof of the concept, we use a liquid crystal mixture MLC 2144 to manufacture a phase shifter and to capture the optical images in a rapid succession by electrically tuning the applied voltage of the phase shifter. Experimental results demonstrate that this technique is capable of reconstructing high-resolution phase images and to realize the thickness profile of a microlens array quantitatively. PMID:28387382

Isotropic differential phase contrast microscopy for quantitative phase bio-imaging.

PubMed

Chen, Hsi-Hsun; Lin, Yu-Zi; Luo, Yuan

2018-05-16

Quantitative phase imaging (QPI) has been investigated to retrieve optical phase information of an object and applied to biological microscopy and related medical studies. In recent examples, differential phase contrast (DPC) microscopy can recover phase image of thin sample under multi-axis intensity measurements in wide-field scheme. Unlike conventional DPC, based on theoretical approach under partially coherent condition, we propose a new method to achieve isotropic differential phase contrast (iDPC) with high accuracy and stability for phase recovery in simple and high-speed fashion. The iDPC is simply implemented with a partially coherent microscopy and a programmable thin-film transistor (TFT) shield to digitally modulate structured illumination patterns for QPI. In this article, simulation results show consistency of our theoretical approach for iDPC under partial coherence. In addition, we further demonstrate experiments of quantitative phase images of a standard micro-lens array, as well as label-free live human cell samples. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Smartphone based hand-held quantitative phase microscope using the transport of intensity equation method.

PubMed

Meng, Xin; Huang, Huachuan; Yan, Keding; Tian, Xiaolin; Yu, Wei; Cui, Haoyang; Kong, Yan; Xue, Liang; Liu, Cheng; Wang, Shouyu

2016-12-20

In order to realize high contrast imaging with portable devices for potential mobile healthcare, we demonstrate a hand-held smartphone based quantitative phase microscope using the transport of intensity equation method. With a cost-effective illumination source and compact microscope system, multi-focal images of samples can be captured by the smartphone's camera via manual focusing. Phase retrieval is performed using a self-developed Android application, which calculates sample phases from multi-plane intensities via solving the Poisson equation. We test the portable microscope using a random phase plate with known phases, and to further demonstrate its performance, a red blood cell smear, a Pap smear and monocot root and broad bean epidermis sections are also successfully imaged. Considering its advantages as an accurate, high-contrast, cost-effective and field-portable device, the smartphone based hand-held quantitative phase microscope is a promising tool which can be adopted in the future in remote healthcare and medical diagnosis.

Diffraction enhance x-ray imaging for quantitative phase contrast studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Agrawal, A. K.; Singh, B., E-mail: balwants@rrcat.gov.in; Kashyap, Y. S.

2016-05-23

Conventional X-ray imaging based on absorption contrast permits limited visibility of feature having small density and thickness variations. For imaging of weakly absorbing material or materials possessing similar densities, a novel phase contrast imaging techniques called diffraction enhanced imaging has been designed and developed at imaging beamline Indus-2 RRCAT Indore. The technique provides improved visibility of the interfaces and show high contrast in the image forsmall density or thickness gradients in the bulk. This paper presents basic principle, instrumentation and analysis methods for this technique. Initial results of quantitative phase retrieval carried out on various samples have also been presented.

Multimodal computational microscopy based on transport of intensity equation

NASA Astrophysics Data System (ADS)

Li, Jiaji; Chen, Qian; Sun, Jiasong; Zhang, Jialin; Zuo, Chao

2016-12-01

Transport of intensity equation (TIE) is a powerful tool for phase retrieval and quantitative phase imaging, which requires intensity measurements only at axially closely spaced planes without a separate reference beam. It does not require coherent illumination and works well on conventional bright-field microscopes. The quantitative phase reconstructed by TIE gives valuable information that has been encoded in the complex wave field by passage through a sample of interest. Such information may provide tremendous flexibility to emulate various microscopy modalities computationally without requiring specialized hardware components. We develop a requisite theory to describe such a hybrid computational multimodal imaging system, which yields quantitative phase, Zernike phase contrast, differential interference contrast, and light field moment imaging, simultaneously. It makes the various observations for biomedical samples easy. Then we give the experimental demonstration of these ideas by time-lapse imaging of live HeLa cell mitosis. Experimental results verify that a tunable lens-based TIE system, combined with the appropriate postprocessing algorithm, can achieve a variety of promising imaging modalities in parallel with the quantitative phase images for the dynamic study of cellular processes.

Quantitative, Comparable Coherent Anti-Stokes Raman Scattering (CARS) Spectroscopy: Correcting Errors in Phase Retrieval

PubMed Central

Camp, Charles H.; Lee, Young Jong; Cicerone, Marcus T.

2017-01-01

Coherent anti-Stokes Raman scattering (CARS) microspectroscopy has demonstrated significant potential for biological and materials imaging. To date, however, the primary mechanism of disseminating CARS spectroscopic information is through pseudocolor imagery, which explicitly neglects a vast majority of the hyperspectral data. Furthermore, current paradigms in CARS spectral processing do not lend themselves to quantitative sample-to-sample comparability. The primary limitation stems from the need to accurately measure the so-called nonresonant background (NRB) that is used to extract the chemically-sensitive Raman information from the raw spectra. Measurement of the NRB on a pixel-by-pixel basis is a nontrivial task; thus, reference NRB from glass or water are typically utilized, resulting in error between the actual and estimated amplitude and phase. In this manuscript, we present a new methodology for extracting the Raman spectral features that significantly suppresses these errors through phase detrending and scaling. Classic methods of error-correction, such as baseline detrending, are demonstrated to be inaccurate and to simply mask the underlying errors. The theoretical justification is presented by re-developing the theory of phase retrieval via the Kramers-Kronig relation, and we demonstrate that these results are also applicable to maximum entropy method-based phase retrieval. This new error-correction approach is experimentally applied to glycerol spectra and tissue images, demonstrating marked consistency between spectra obtained using different NRB estimates, and between spectra obtained on different instruments. Additionally, in order to facilitate implementation of these approaches, we have made many of the tools described herein available free for download. PMID:28819335

Characterization of a high-energy in-line phase contrast tomosynthesis prototype.

PubMed

Wu, Di; Yan, Aimin; Li, Yuhua; Wong, Molly D; Zheng, Bin; Wu, Xizeng; Liu, Hong

2015-05-01

In this research, a high-energy in-line phase contrast tomosynthesis prototype was developed and characterized through quantitative investigations and phantom studies. The prototype system consists of an x-ray source, a motorized rotation stage, and a CMOS detector with a pixel pitch of 0.05 mm. The x-ray source was operated at 120 kVp for this study, and the objects were mounted on the rotation stage 76.2 cm (R1) from the source and 114.3 cm (R2) from the detector. The large air gap between the object and detector guarantees sufficient phase-shift effects. The quantitative evaluation of this prototype included modulation transfer function and noise power spectrum measurements conducted under both projection mode and tomosynthesis mode. Phantom studies were performed including three custom designed phantoms with complex structures: a five-layer bubble wrap phantom, a fishbone phantom, and a chicken breast phantom with embedded fibrils and mass structures extracted from an ACR phantom. In-plane images of the phantoms were acquired to investigate their image qualities through observation, intensity profile plots, edge enhancement evaluations, and/or contrast-to-noise ratio calculations. In addition, the robust phase-attenuation duality (PAD)-based phase retrieval method was applied to tomosynthesis for the first time in this research. It was utilized as a preprocessing method to fully exhibit phase contrast on the angular projection before reconstruction. The resolution and noise characteristics of this high-energy in-line phase contrast tomosynthesis prototype were successfully investigated and demonstrated. The phantom studies demonstrated that this imaging prototype can successfully remove the structure overlapping in phantom projections, obtain delineate interfaces, and achieve better contrast-to-noise ratio after applying phase retrieval to the angular projections. This research successfully demonstrated a high-energy in-line phase contrast tomosynthesis prototype. In addition, the PAD-based method of phase retrieval was combined with tomosynthesis imaging for the first time, which demonstrated its capability in significantly improving the contrast-to-noise ratios in the images.

Quantitative phase microscopy using deep neural networks

NASA Astrophysics Data System (ADS)

Li, Shuai; Sinha, Ayan; Lee, Justin; Barbastathis, George

2018-02-01

Deep learning has been proven to achieve ground-breaking accuracy in various tasks. In this paper, we implemented a deep neural network (DNN) to achieve phase retrieval in a wide-field microscope. Our DNN utilized the residual neural network (ResNet) architecture and was trained using the data generated by a phase SLM. The results showed that our DNN was able to reconstruct the profile of the phase target qualitatively. In the meantime, large error still existed, which indicated that our approach still need to be improved.

Quantitative Imaging of Single Unstained Magnetotactic Bacteria by Coherent X-ray Diffraction Microscopy.

PubMed

Fan, Jiadong; Sun, Zhibin; Zhang, Jian; Huang, Qingjie; Yao, Shengkun; Zong, Yunbing; Kohmura, Yoshiki; Ishikawa, Tetsuya; Liu, Hong; Jiang, Huaidong

2015-06-16

Novel coherent diffraction microscopy provides a powerful lensless imaging method to obtain a better understanding of the microorganism at the nanoscale. Here we demonstrated quantitative imaging of intact unstained magnetotactic bacteria using coherent X-ray diffraction microscopy combined with an iterative phase retrieval algorithm. Although the signal-to-noise ratio of the X-ray diffraction pattern from single magnetotactic bacterium is weak due to low-scattering ability of biomaterials, an 18.6 nm half-period resolution of reconstructed image was achieved by using a hybrid input-output phase retrieval algorithm. On the basis of the quantitative reconstructed images, the morphology and some intracellular structures, such as nucleoid, polyβ-hydroxybutyrate granules, and magnetosomes, were identified, which were also confirmed by scanning electron microscopy and energy dispersive spectroscopy. With the benefit from the quantifiability of coherent diffraction imaging, for the first time to our knowledge, an average density of magnetotactic bacteria was calculated to be ∼1.19 g/cm(3). This technique has a wide range of applications, especially in quantitative imaging of low-scattering biomaterials and multicomponent materials at nanoscale resolution. Combined with the cryogenic technique or X-ray free electron lasers, the method could image cells in a hydrated condition, which helps to maintain their natural structure.

Identification of ginseng root using quantitative X-ray microtomography.

PubMed

Ye, Linlin; Xue, Yanling; Wang, Yudan; Qi, Juncheng; Xiao, Tiqiao

2017-07-01

The use of X-ray phase-contrast microtomography for the investigation of Chinese medicinal materials is advantageous for its nondestructive, in situ , and three-dimensional quantitative imaging properties. The X-ray phase-contrast microtomography quantitative imaging method was used to investigate the microstructure of ginseng, and the phase-retrieval method is also employed to process the experimental data. Four different ginseng samples were collected and investigated; these were classified according to their species, production area, and sample growth pattern. The quantitative internal characteristic microstructures of ginseng were extracted successfully. The size and position distributions of the calcium oxalate cluster crystals (COCCs), important secondary metabolites that accumulate in ginseng, are revealed by the three-dimensional quantitative imaging method. The volume and amount of the COCCs in different species of the ginseng are obtained by a quantitative analysis of the three-dimensional microstructures, which shows obvious difference among the four species of ginseng. This study is the first to provide evidence of the distribution characteristics of COCCs to identify four types of ginseng, with regard to species authentication and age identification, by X-ray phase-contrast microtomography quantitative imaging. This method is also expected to reveal important relationships between COCCs and the occurrence of the effective medicinal components of ginseng.

High-speed and high-resolution quantitative phase imaging with digital-micromirror device-based illumination (Conference Presentation)

NASA Astrophysics Data System (ADS)

Zhou, Renjie; Jin, Di; Yaqoob, Zahid; So, Peter T. C.

2017-02-01

Due to the large number of available mirrors, the patterning speed, low-cost, and compactness, digital-micromirror devices (DMDs) have been extensively used in biomedical imaging system. Recently, DMDs have been brought to the quantitative phase microscopy (QPM) field to achieve synthetic-aperture imaging and tomographic imaging. Last year, our group demonstrated using DMD for QPM, where the phase-retrieval is based on a recently developed Fourier ptychography algorithm. In our previous system, the illumination angle was varied through coding the aperture plane of the illumination system, which has a low efficiency on utilizing the laser power. In our new DMD-based QPM system, we use the Lee-holograms, which is conjugated to the sample plane, to change the illumination angles for much higher power efficiency. Multiple-angle illumination can also be achieved with this method. With this versatile system, we can achieve FPM-based high-resolution phase imaging with 250 nm lateral resolution using the Rayleigh criteria. Due to the use of a powerful laser, the imaging speed would only be limited by the camera acquisition speed. With a fast camera, we expect to achieve close to 100 fps phase imaging speed that has not been achieved in current FPM imaging systems. By adding reference beam, we also expect to achieve synthetic-aperture imaging while directly measuring the phase of the sample fields. This would reduce the phase-retrieval processing time to allow for real-time imaging applications in the future.

Chemotaxis of cancer cells in three-dimensional environment monitored label-free by quantitative phase digital holographic microscopy

NASA Astrophysics Data System (ADS)

Kemper, Björn; Schnekenburger, Jürgen; Ketelhut, Steffi

2017-02-01

We investigated the capabilities of digital holographic microscopy (DHM) for label-free quantification of the response of living single cells to chemical stimuli in 3D assays. Fibro sarcoma cells were observed in a collagen matrix inside 3D chemotaxis chambers with a Mach-Zehnder interferometer-based DHM setup. From the obtained series of quantitative phase images, the migration trajectories of single cells were retrieved by automated cell tracking and subsequently analyzed for maximum migration distance and motility. Our results demonstrate DHM as a highly reliable and efficient tool for label-free quantification of chemotaxis in 2D and 3D environments.

The importance of the boundary condition in the transport of intensity equation based phase measurement

NASA Astrophysics Data System (ADS)

Zhang, Jialin; Chen, Qian; Li, Jiaji; Zuo, Chao

2017-02-01

The transport of intensity equation (TIE) is a powerful tool for direct quantitative phase retrieval in microscopy imaging. However, there may be some problems when dealing with the boundary condition of the TIE. The previous work introduces a hard-edged aperture to the camera port of the traditional bright field microscope to generate the boundary signal for the TIE solver. Under this Neumann boundary condition, we can obtain the quantitative phase without any assumption or prior knowledge about the test object and the setup. In this paper, we will demonstrate the effectiveness of this method based on some experiments in practice. The micro lens array will be used for the comparison of two TIE solvers results based on introducing the aperture or not and this accurate quantitative phase imaging technique allows measuring cell dry mass which is used in biology to follow cell cycle, to investigate cell metabolism, or to address effects of drugs.

Combined multi-plane phase retrieval and super-resolution optical fluctuation imaging for 4D cell microscopy

NASA Astrophysics Data System (ADS)

Descloux, A.; Grußmayer, K. S.; Bostan, E.; Lukes, T.; Bouwens, A.; Sharipov, A.; Geissbuehler, S.; Mahul-Mellier, A.-L.; Lashuel, H. A.; Leutenegger, M.; Lasser, T.

2018-03-01

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going `beyond the diffraction barrier' comes at a price, since most far-field super-resolution imaging techniques trade temporal for spatial super-resolution. We propose the combination of a novel label-free white light quantitative phase imaging with fluorescence to provide high-speed imaging and spatial super-resolution. The non-iterative phase retrieval relies on the acquisition of single images at each z-location and thus enables straightforward 3D phase imaging using a classical microscope. We realized multi-plane imaging using a customized prism for the simultaneous acquisition of eight planes. This allowed us to not only image live cells in 3D at up to 200 Hz, but also to integrate fluorescence super-resolution optical fluctuation imaging within the same optical instrument. The 4D microscope platform unifies the sensitivity and high temporal resolution of phase imaging with the specificity and high spatial resolution of fluorescence microscopy.

X-ray phase contrast tomography from whole organ down to single cells

NASA Astrophysics Data System (ADS)

Krenkel, Martin; Töpperwien, Mareike; Bartels, Matthias; Lingor, Paul; Schild, Detlev; Salditt, Tim

2014-09-01

We use propagation based hard x-ray phase contrast tomography to explore the three dimensional structure of neuronal tissues from the organ down to sub-cellular level, based on combinations of synchrotron radiation and laboratory sources. To this end a laboratory based microfocus tomography setup has been built in which the geometry was optimized for phase contrast imaging and tomography. By utilizing phase retrieval algorithms, quantitative reconstructions can be obtained that enable automatic renderings without edge artifacts. A high brightness liquid metal microfocus x-ray source in combination with a high resolution detector yielding a resolution down to 1.5 μm. To extend the method to nanoscale resolution we use a divergent x-ray waveguide beam geometry at the synchrotron. Thus, the magnification can be easily tuned by placing the sample at different defocus distances. Due to the small Fresnel numbers in this geometry the measured images are of holographic nature which poses a challenge in phase retrieval.

Technical Note: Synchrotron-based high-energy x-ray phase sensitive microtomography for biomedical research

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Huiqiang; Wu, Xizeng, E-mail: xwu@uabmc.edu, E-mail: tqxiao@sinap.ac.cn; Xiao, Tiqiao, E-mail: xwu@uabmc.edu, E-mail: tqxiao@sinap.ac.cn

Purpose: Propagation-based phase-contrast CT (PPCT) utilizes highly sensitive phase-contrast technology applied to x-ray microtomography. Performing phase retrieval on the acquired angular projections can enhance image contrast and enable quantitative imaging. In this work, the authors demonstrate the validity and advantages of a novel technique for high-resolution PPCT by using the generalized phase-attenuation duality (PAD) method of phase retrieval. Methods: A high-resolution angular projection data set of a fish head specimen was acquired with a monochromatic 60-keV x-ray beam. In one approach, the projection data were directly used for tomographic reconstruction. In two other approaches, the projection data were preprocessed bymore » phase retrieval based on either the linearized PAD method or the generalized PAD method. The reconstructed images from all three approaches were then compared in terms of tissue contrast-to-noise ratio and spatial resolution. Results: The authors’ experimental results demonstrated the validity of the PPCT technique based on the generalized PAD-based method. In addition, the results show that the authors’ technique is superior to the direct PPCT technique as well as the linearized PAD-based PPCT technique in terms of their relative capabilities for tissue discrimination and characterization. Conclusions: This novel PPCT technique demonstrates great potential for biomedical imaging, especially for applications that require high spatial resolution and limited radiation exposure.« less

Characterization of a high-energy in-line phase contrast tomosynthesis prototype

PubMed Central

Wu, Di; Yan, Aimin; Li, Yuhua; Wong, Molly D.; Zheng, Bin; Wu, Xizeng; Liu, Hong

2015-01-01

Purpose: In this research, a high-energy in-line phase contrast tomosynthesis prototype was developed and characterized through quantitative investigations and phantom studies. Methods: The prototype system consists of an x-ray source, a motorized rotation stage, and a CMOS detector with a pixel pitch of 0.05 mm. The x-ray source was operated at 120 kVp for this study, and the objects were mounted on the rotation stage 76.2 cm (R1) from the source and 114.3 cm (R2) from the detector. The large air gap between the object and detector guarantees sufficient phase-shift effects. The quantitative evaluation of this prototype included modulation transfer function and noise power spectrum measurements conducted under both projection mode and tomosynthesis mode. Phantom studies were performed including three custom designed phantoms with complex structures: a five-layer bubble wrap phantom, a fishbone phantom, and a chicken breast phantom with embedded fibrils and mass structures extracted from an ACR phantom. In-plane images of the phantoms were acquired to investigate their image qualities through observation, intensity profile plots, edge enhancement evaluations, and/or contrast-to-noise ratio calculations. In addition, the robust phase-attenuation duality (PAD)-based phase retrieval method was applied to tomosynthesis for the first time in this research. It was utilized as a preprocessing method to fully exhibit phase contrast on the angular projection before reconstruction. Results: The resolution and noise characteristics of this high-energy in-line phase contrast tomosynthesis prototype were successfully investigated and demonstrated. The phantom studies demonstrated that this imaging prototype can successfully remove the structure overlapping in phantom projections, obtain delineate interfaces, and achieve better contrast-to-noise ratio after applying phase retrieval to the angular projections. Conclusions: This research successfully demonstrated a high-energy in-line phase contrast tomosynthesis prototype. In addition, the PAD-based method of phase retrieval was combined with tomosynthesis imaging for the first time, which demonstrated its capability in significantly improving the contrast-to-noise ratios in the images. PMID:25979035

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Damao; Wang, Zhien; Heymsfield, Andrew J.

Measurement of ice number concentration in clouds is important but still challenging. Stratiform mixed-phase clouds (SMCs) provide a simple scenario for retrieving ice number concentration from remote sensing measurements. The simple ice generation and growth pattern in SMCs offers opportunities to use cloud radar reflectivity (Ze) measurements and other cloud properties to infer ice number concentration quantitatively. To understand the strong temperature dependency of ice habit and growth rate quantitatively, we develop a 1-D ice growth model to calculate the ice diffusional growth along its falling trajectory in SMCs. The radar reflectivity and fall velocity profiles of ice crystals calculatedmore » from the 1-D ice growth model are evaluated with the Atmospheric Radiation Measurements (ARM) Climate Research Facility (ACRF) ground-based high vertical resolution radar measurements. Combining Ze measurements and 1-D ice growth model simulations, we develop a method to retrieve the ice number concentrations in SMCs at given cloud top temperature (CTT) and liquid water path (LWP). The retrieved ice concentrations in SMCs are evaluated with in situ measurements and with a three-dimensional cloud-resolving model simulation with a bin microphysical scheme. These comparisons show that the retrieved ice number concentrations are within an uncertainty of a factor of 2, statistically.« less

Hyperspectral and differential CARS microscopy for quantitative chemical imaging in human adipocytes

PubMed Central

Di Napoli, Claudia; Pope, Iestyn; Masia, Francesco; Watson, Peter; Langbein, Wolfgang; Borri, Paola

2014-01-01

In this work, we demonstrate the applicability of coherent anti-Stokes Raman scattering (CARS) micro-spectroscopy for quantitative chemical imaging of saturated and unsaturated lipids in human stem-cell derived adipocytes. We compare dual-frequency/differential CARS (D-CARS), which enables rapid imaging and simple data analysis, with broadband hyperspectral CARS microscopy analyzed using an unsupervised phase-retrieval and factorization method recently developed by us for quantitative chemical image analysis. Measurements were taken in the vibrational fingerprint region (1200–2000/cm) and in the CH stretch region (2600–3300/cm) using a home-built CARS set-up which enables hyperspectral imaging with 10/cm resolution via spectral focussing from a single broadband 5 fs Ti:Sa laser source. Through a ratiometric analysis, both D-CARS and phase-retrieved hyperspectral CARS determine the concentration of unsaturated lipids with comparable accuracy in the fingerprint region, while in the CH stretch region D-CARS provides only a qualitative contrast owing to its non-linear behavior. When analyzing hyperspectral CARS images using the blind factorization into susceptibilities and concentrations of chemical components recently demonstrated by us, we are able to determine vol:vol concentrations of different lipid components and spatially resolve inhomogeneities in lipid composition with superior accuracy compared to state-of-the art ratiometric methods. PMID:24877002

Refractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus.

PubMed

Lee, SangYun; Park, HyunJoo; Kim, Kyoohyun; Sohn, YongHak; Jang, Seongsoo; Park, YongKeun

2017-04-21

In this paper, we present the optical characterisations of diabetic red blood cells (RBCs) in a non-invasive manner employing three-dimensional (3-D) quantitative phase imaging. By measuring 3-D refractive index tomograms and 2-D time-series phase images, the morphological (volume, surface area and sphericity), biochemical (haemoglobin concentration and content) and mechanical (membrane fluctuation) parameters were quantitatively retrieved at the individual cell level. With simultaneous measurements of individual cell properties, systematic correlative analyses on retrieved RBC parameters were also performed. Our measurements show there exist no statistically significant alterations in morphological and biochemical parameters of diabetic RBCs, compared to those of healthy (non-diabetic) RBCs. In contrast, membrane deformability of diabetic RBCs is significantly lower than that of healthy, non-diabetic RBCs. Interestingly, non-diabetic RBCs exhibit strong correlations between the elevated glycated haemoglobin in RBC cytoplasm and decreased cell deformability, whereas diabetic RBCs do not show correlations. Our observations strongly support the idea that slow and irreversible glycation of haemoglobin and membrane proteins of RBCs by hyperglycaemia significantly compromises RBC deformability in diabetic patients.

Focal length calibration of an electrically tunable lens by digital holography.

PubMed

Wang, Zhaomin; Qu, Weijuan; Yang, Fang; Asundi, Anand Krishna

2016-02-01

The electrically tunable lens (ETL) is a novel current-controlled adaptive optical component which can continuously tune its focus in a specific range via changing its surface curvature. To quantitatively characterize its tuning power, here we assume the ETL to be a pure phase object and present a novel calibration method to dynamically measure its wavefront by use of digital holographic microscopy (DHM). The least squares method is then used to fit the radius of curvature of the wavefront. The focal length is obtained by substituting the radius into the Zemax model of the ETL. The behavior curve between the focal length of the ETL and its driven current is drawn, and a quadratic mathematic model is set up to characterize it. To verify our model, an ETL and offset lens combination is proposed and applied to ETL-based transport of intensity equation (TIE) phase retrieval microscopy. The experimental result demonstrates the calibration works well in TIE phase retrieval in comparison with the phase measured by DHM.

Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging.

PubMed

Zhang, Qinnan; Zhong, Liyun; Tang, Ping; Yuan, Yingjie; Liu, Shengde; Tian, Jindong; Lu, Xiaoxu

2017-05-31

Cell refractive index, an intrinsic optical parameter, is closely correlated with the intracellular mass and concentration. By combining optical phase-shifting interferometry (PSI) and atomic force microscope (AFM) imaging, we constructed a label free, non-invasive and quantitative refractive index of single cell measurement system, in which the accurate phase map of single cell was retrieved with PSI technique and the cell morphology with nanoscale resolution was achieved with AFM imaging. Based on the proposed AFM/PSI system, we achieved quantitative refractive index distributions of single red blood cell and Jurkat cell, respectively. Further, the quantitative change of refractive index distribution during Daunorubicin (DNR)-induced Jurkat cell apoptosis was presented, and then the content changes of intracellular biochemical components were achieved. Importantly, these results were consistent with Raman spectral analysis, indicating that the proposed PSI/AFM based refractive index system is likely to become a useful tool for intracellular biochemical components analysis measurement, and this will facilitate its application for revealing cell structure and pathological state from a new perspective.

Quantitative monitoring of activity-dependent bulk endocytosis of synaptic vesicle membrane by fluorescent dextran imaging

PubMed Central

Clayton, Emma Louise; Cousin, Michael Alan

2012-01-01

Activity-dependent bulk endocytosis (ADBE) is the dominant synaptic vesicle (SV) retrieval mode in central nerve terminals during periods of intense neuronal activity. Despite this fact there are very few real time assays that report the activity of this critical SV retrieval mode. In this paper we report a simple and quantitative assay of ADBE using uptake of large flourescent dextrans as fluid phase markers. We show that almost all dextran uptake occurs in nerve terminals, using co-localisation with the fluorescent probe FM1-43. We also demonstrate that accumulated dextran cannot be unloaded by neuronal stimulation, indicating its specific loading into bulk endosomes and not SVs. Quantification of dextran uptake was achieved by using thresholding analysis to count the number of loaded nerve terminals, since monitoring the average fluorescence intensity of these nerve terminals did not accurately report the extent of ADBE. Using this analysis we showed that dextran uptake occurs very soon after stimulation and that it does not persist when stimulation terminates. Thus we have devised a simple and quantitative method to monitor ADBE in living neurones, which will be ideal for real time screening of small molecule inhibitors of this key SV retrieval mode. PMID:19766140

Shot noise-limited Cramér-Rao bound and algorithmic sensitivity for wavelength shifting interferometry

NASA Astrophysics Data System (ADS)

Chen, Shichao; Zhu, Yizheng

2017-02-01

Sensitivity is a critical index to measure the temporal fluctuation of the retrieved optical pathlength in quantitative phase imaging system. However, an accurate and comprehensive analysis for sensitivity evaluation is still lacking in current literature. In particular, previous theoretical studies for fundamental sensitivity based on Gaussian noise models are not applicable to modern cameras and detectors, which are dominated by shot noise. In this paper, we derive two shot noiselimited theoretical sensitivities, Cramér-Rao bound and algorithmic sensitivity for wavelength shifting interferometry, which is a major category of on-axis interferometry techniques in quantitative phase imaging. Based on the derivations, we show that the shot noise-limited model permits accurate estimation of theoretical sensitivities directly from measured data. These results can provide important insights into fundamental constraints in system performance and can be used to guide system design and optimization. The same concepts can be generalized to other quantitative phase imaging techniques as well.

DEVELOPMENT OF AN IN SITU THERMAL EXTRACTION DETECTION SYSTEM (TEDS) FOR RAPID, ACCURATE, QUANTITATIVE ANALYSIS OF ENVIRONMENTAL POLLUTANTS IN THE SUBSURFACE - PHASE I

EPA Science Inventory

Ion Signature Technology, Inc. (IST) will develop and market a collection and analysis system that will retrieve soil-bound pollutants as well as soluble and non-soluble contaminants from groundwater as the probe is pushed by cone penetrometry of Geoprobe into the subsurface. ...

X-ray computed tomography of wood-adhesive bondlines: Attenuation and phase-contrast effects

DOE PAGES

Paris, Jesse L.; Kamke, Frederick A.; Xiao, Xianghui

2015-07-29

Microscale X-ray computed tomography (XCT) is discussed as a technique for identifying 3D adhesive distribution in wood-adhesive bondlines. Visualization and material segmentation of the adhesives from the surrounding cellular structures require sufficient gray-scale contrast in the reconstructed XCT data. Commercial wood-adhesive polymers have similar chemical characteristics and density to wood cell wall polymers and therefore do not provide good XCT attenuation contrast in their native form. Here, three different adhesive types, namely phenol formaldehyde, polymeric diphenylmethane diisocyanate, and a hybrid polyvinyl acetate, are tagged with iodine such that they yield sufficient X-ray attenuation contrast. However, phase-contrast effects at material edgesmore » complicate image quality and segmentation in XCT data reconstructed with conventional filtered backprojection absorption contrast algorithms. A quantitative phase retrieval algorithm, which isolates and removes the phase-contrast effect, was demonstrated. The paper discusses and illustrates the balance between material X-ray attenuation and phase-contrast effects in all quantitative XCT analyses of wood-adhesive bondlines.« less

X-ray computed tomography of wood-adhesive bondlines: Attenuation and phase-contrast effects

DOE Office of Scientific and Technical Information (OSTI.GOV)

Paris, Jesse L.; Kamke, Frederick A.; Xiao, Xianghui

Microscale X-ray computed tomography (XCT) is discussed as a technique for identifying 3D adhesive distribution in wood-adhesive bondlines. Visualization and material segmentation of the adhesives from the surrounding cellular structures require sufficient gray-scale contrast in the reconstructed XCT data. Commercial wood-adhesive polymers have similar chemical characteristics and density to wood cell wall polymers and therefore do not provide good XCT attenuation contrast in their native form. Here, three different adhesive types, namely phenol formaldehyde, polymeric diphenylmethane diisocyanate, and a hybrid polyvinyl acetate, are tagged with iodine such that they yield sufficient X-ray attenuation contrast. However, phase-contrast effects at material edgesmore » complicate image quality and segmentation in XCT data reconstructed with conventional filtered backprojection absorption contrast algorithms. A quantitative phase retrieval algorithm, which isolates and removes the phase-contrast effect, was demonstrated. The paper discusses and illustrates the balance between material X-ray attenuation and phase-contrast effects in all quantitative XCT analyses of wood-adhesive bondlines.« less

Sensing dynamic cytoplasm refractive index changes of adherent cells with quantitative phase microscopy using incorporated microspheres as optical probes.

PubMed

Przibilla, Sabine; Dartmann, Sebastian; Vollmer, Angelika; Ketelhut, Steffi; Greve, Burkhard; von Bally, Gert; Kemper, Björn

2012-09-01

The intracellular refractive index is an important parameter that describes the optical density of the cytoplasm and the concentration of the intracellular solutes. The refractive index of adherently grown cells is difficult to access. We present a method in which silica microspheres in living cells are used to determine the cytoplasm refractive index with quantitative phase microscopy. The reliability of our approach for refractive index retrieval is shown by data from a comparative study on osmotically stimulated adherent and suspended human pancreatic tumor cells. Results from adherent human fibro sarcoma cells demonstrate the capability of the method for sensing of dynamic refractive index changes and its usage with microfluidics.

Lung cancer diagnosis with quantitative DIC microscopy and support vector machine

NASA Astrophysics Data System (ADS)

Zheng, Longfei; Cai, Shuangshuang; Zeng, Bixin; Xu, Min

2017-01-01

We report the study of lung squamous cell carcinoma diagnosis using the TI-DIC microscopy and the scattering-phase theorem. The spatially resolved optical properties of tissue are computed from the 2D phase map via the scattering-phase theorem. The scattering coefficient, the reduced scattering coefficient, and the anisotropy factor are all found to increase with the grade of lung cancer. The retrieved optical parameters are shown to distinguish cancer cases from the normal cases with high accuracy. This label-free microscopic approach applicable to fresh tissues may be promising for in situ rapid cancer diagnosis.

Probing myocardium biomechanics using quantitative optical coherence elastography

NASA Astrophysics Data System (ADS)

Wang, Shang; Lopez, Andrew L.; Morikawa, Yuka; Tao, Ge; Li, Jiasong; Larina, Irina V.; Martin, James F.; Larin, Kirill V.

2015-03-01

We present a quantitative optical coherence elastographic method for noncontact assessment of the myocardium elasticity. The method is based on shear wave imaging optical coherence tomography (SWI-OCT), where a focused air-puff system is used to induce localized tissue deformation through a low-pressure short-duration air stream and a phase-sensitive OCT system is utilized to monitor the propagation of the induced tissue displacement with nanoscale sensitivity. The 1-D scanning of M-mode OCT imaging and the application of optical phase retrieval and mapping techniques enable the reconstruction and visualization of 2-D depth-resolved shear wave propagation in tissue with ultra-high frame rate. The feasibility of this method in quantitative elasticity measurement is demonstrated on tissue-mimicking phantoms with the estimated Young's modulus compared with uniaxial compression tests. We also performed pilot experiments on ex vivo mouse cardiac muscle tissues with normal and genetically altered cardiomyocytes. Our results indicate this noncontact quantitative optical coherence elastographic method can be a useful tool for the cardiac muscle research and studies.

Cortisol reactivity and emotional memory after psychosocial stress in oral contraceptive users.

PubMed

Mordecai, Kristen L; Rubin, Leah H; Eatough, Erin; Sundermann, Erin; Drogos, Lauren; Savarese, Antonia; Maki, Pauline M

2017-01-02

Oral contraceptive (OC) users typically show a blunted or no cortisol response to psychosocial stress. Although most OC regimens include both an inactive (dummy) and active pill phase, studies have not systematically investigated cortisol responses during these pill phases. Further, high levels of cortisol following a stressor diminish retrieval of emotional material, but the effects of stress on memory among OC users are poorly understood. We examined the effects of a psychosocial stressor, the Trier Social Stress Test, vs. a control condition on cortisol responsivity and emotional memory retrieval in women tested either during their active (n = 18) or inactive pill phase (n = 21). In secondary analyses, we quantitatively compared OC users with normally cycling women and showed a significant lack of cortisol response during both active and inactive pill phase. Emotional recall did not differ between active and inactive pill phases. Stress differentially diminished recall of negative words compared with positive or neutral words, but cortisol levels were unrelated to memory performance. These findings indicate that OC users have distinct cortisol and memory responses to stress that are similar between the active and inactive pill phases. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

A high throughput geocomputing system for remote sensing quantitative retrieval and a case study

NASA Astrophysics Data System (ADS)

Xue, Yong; Chen, Ziqiang; Xu, Hui; Ai, Jianwen; Jiang, Shuzheng; Li, Yingjie; Wang, Ying; Guang, Jie; Mei, Linlu; Jiao, Xijuan; He, Xingwei; Hou, Tingting

2011-12-01

The quality and accuracy of remote sensing instruments have been improved significantly, however, rapid processing of large-scale remote sensing data becomes the bottleneck for remote sensing quantitative retrieval applications. The remote sensing quantitative retrieval is a data-intensive computation application, which is one of the research issues of high throughput computation. The remote sensing quantitative retrieval Grid workflow is a high-level core component of remote sensing Grid, which is used to support the modeling, reconstruction and implementation of large-scale complex applications of remote sensing science. In this paper, we intend to study middleware components of the remote sensing Grid - the dynamic Grid workflow based on the remote sensing quantitative retrieval application on Grid platform. We designed a novel architecture for the remote sensing Grid workflow. According to this architecture, we constructed the Remote Sensing Information Service Grid Node (RSSN) with Condor. We developed a graphic user interface (GUI) tools to compose remote sensing processing Grid workflows, and took the aerosol optical depth (AOD) retrieval as an example. The case study showed that significant improvement in the system performance could be achieved with this implementation. The results also give a perspective on the potential of applying Grid workflow practices to remote sensing quantitative retrieval problems using commodity class PCs.

Fresnel transform phase retrieval from magnitude.

PubMed

Pitts, Todd A; Greenleaf, James F

2003-08-01

This report presents a generalized projection method for recovering the phase of a finite support, two-dimensional signal from knowledge of its magnitude in the spatial position and Fresnel transform domains. We establish the uniqueness of sampled monochromatic scalar field phase given Fresnel transform magnitude and finite region of support constraints for complex signals. We derive an optimally relaxed version of the algorithm resulting in a significant reduction in the number of iterations needed to obtain useful results. An advantage of using the Fresnel transform (as opposed to Fourier) for measurement is that the shift-invariance of the transform operator implies retention of object location information in the transformed image magnitude. As a practical application in the context of ultrasound beam measurement we discuss the determination of small optical phase shifts from near field optical intensity distributions. Experimental data are used to reconstruct the phase shape of an optical field immediately after propagating through a wide bandwidth ultrasonic pulse. The phase of each point on the optical wavefront is proportional to the ray sum of pressure through the ultrasound pulse (assuming low ultrasonic intensity). An entire pressure field was reconstructed in three dimensions and compared with a calibrated hydrophone measurement. The comparison is excellent, demonstrating that the phase retrieval is quantitative.

Quantitative X-ray dark-field and phase tomography using single directional speckle scanning technique

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Hongchang, E-mail: hongchang.wang@diamond.ac.uk; Kashyap, Yogesh; Sawhney, Kawal

2016-03-21

X-ray dark-field contrast tomography can provide important supplementary information inside a sample to the conventional absorption tomography. Recently, the X-ray speckle based technique has been proposed to provide qualitative two-dimensional dark-field imaging with a simple experimental arrangement. In this letter, we deduce a relationship between the second moment of scattering angle distribution and cross-correlation degradation of speckle and establish a quantitative basis of X-ray dark-field tomography using single directional speckle scanning technique. In addition, the phase contrast images can be simultaneously retrieved permitting tomographic reconstruction, which yields enhanced contrast in weakly absorbing materials. Such complementary tomography technique can allow systematicmore » investigation of complex samples containing both soft and hard materials.« less

Influence of sample preparation and reliability of automated numerical refocusing in stain-free analysis of dissected tissues with quantitative phase digital holographic microscopy

NASA Astrophysics Data System (ADS)

Kemper, Björn; Lenz, Philipp; Bettenworth, Dominik; Krausewitz, Philipp; Domagk, Dirk; Ketelhut, Steffi

2015-05-01

Digital holographic microscopy (DHM) has been demonstrated to be a versatile tool for high resolution non-destructive quantitative phase imaging of surfaces and multi-modal minimally-invasive monitoring of living cell cultures in-vitro. DHM provides quantitative monitoring of physiological processes through functional imaging and structural analysis which, for example, gives new insight into signalling of cellular water permeability and cell morphology changes due to toxins and infections. Also the analysis of dissected tissues quantitative DHM phase contrast prospects application fields by stain-free imaging and the quantification of tissue density changes. We show that DHM allows imaging of different tissue layers with high contrast in unstained tissue sections. As the investigation of fixed samples represents a very important application field in pathology, we also analyzed the influence of the sample preparation. The retrieved data demonstrate that the quality of quantitative DHM phase images of dissected tissues depends strongly on the fixing method and common staining agents. As in DHM the reconstruction is performed numerically, multi-focus imaging is achieved from a single digital hologram. Thus, we evaluated the automated refocussing feature of DHM for application on different types of dissected tissues and revealed that on moderately stained samples highly reproducible holographic autofocussing can be achieved. Finally, it is demonstrated that alterations of the spatial refractive index distribution in murine and human tissue samples represent a reliable absolute parameter that is related of different degrees of inflammation in experimental colitis and Crohn's disease. This paves the way towards the usage of DHM in digital pathology for automated histological examinations and further studies to elucidate the translational potential of quantitative phase microscopy for the clinical management of patients, e.g., with inflammatory bowel disease.

Phase-space evolution of x-ray coherence in phase-sensitive imaging.

PubMed

Wu, Xizeng; Liu, Hong

2008-08-01

X-ray coherence evolution in the imaging process plays a key role for x-ray phase-sensitive imaging. In this work we present a phase-space formulation for the phase-sensitive imaging. The theory is reformulated in terms of the cross-spectral density and associated Wigner distribution. The phase-space formulation enables an explicit and quantitative account of partial coherence effects on phase-sensitive imaging. The presented formulas for x-ray spectral density at the detector can be used for performing accurate phase retrieval and optimizing the phase-contrast visibility. The concept of phase-space shearing length derived from this phase-space formulation clarifies the spatial coherence requirement for phase-sensitive imaging with incoherent sources. The theory has been applied to x-ray Talbot interferometric imaging as well. The peak coherence condition derived reveals new insights into three-grating-based Talbot-interferometric imaging and gratings-based x-ray dark-field imaging.

Quantitative phase measurement for wafer-level optics

NASA Astrophysics Data System (ADS)

Qu, Weijuan; Wen, Yongfu; Wang, Zhaomin; Yang, Fang; Huang, Lei; Zuo, Chao

2015-07-01

Wafer-level-optics now is widely used in smart phone camera, mobile video conferencing or in medical equipment that require tiny cameras. Extracting quantitative phase information has received increased interest in order to quantify the quality of manufactured wafer-level-optics, detect defective devices before packaging, and provide feedback for manufacturing process control, all at the wafer-level for high-throughput microfabrication. We demonstrate two phase imaging methods, digital holographic microscopy (DHM) and Transport-of-Intensity Equation (TIE) to measure the phase of the wafer-level lenses. DHM is a laser-based interferometric method based on interference of two wavefronts. It can perform a phase measurement in a single shot. While a minimum of two measurements of the spatial intensity of the optical wave in closely spaced planes perpendicular to the direction of propagation are needed to do the direct phase retrieval by solving a second-order differential equation, i.e., with a non-iterative deterministic algorithm from intensity measurements using the Transport-of-Intensity Equation (TIE). But TIE is a non-interferometric method, thus can be applied to partial-coherence light. We demonstrated the capability and disability for the two phase measurement methods for wafer-level optics inspection.

Quantitative evaluation of hidden defects in cast iron components using ultrasound activated lock-in vibrothermography.

PubMed

Montanini, R; Freni, F; Rossi, G L

2012-09-01

This paper reports one of the first experimental results on the application of ultrasound activated lock-in vibrothermography for quantitative assessment of buried flaws in complex cast parts. The use of amplitude modulated ultrasonic heat generation allowed selective response of defective areas within the part, as the defect itself is turned into a local thermal wave emitter. Quantitative evaluation of hidden damages was accomplished by estimating independently both the area and the depth extension of the buried flaws, while x-ray 3D computed tomography was used as reference for sizing accuracy assessment. To retrieve flaw's area, a simple yet effective histogram-based phase image segmentation algorithm with automatic pixels classification has been developed. A clear correlation was found between the thermal (phase) signature measured by the infrared camera on the target surface and the actual mean cross-section area of the flaw. Due to the very fast cycle time (<30 s/part), the method could potentially be applied for 100% quality control of casting components.

Propagation based phase retrieval of simulated intensity measurements using artificial neural networks

NASA Astrophysics Data System (ADS)

Kemp, Z. D. C.

2018-04-01

Determining the phase of a wave from intensity measurements has many applications in fields such as electron microscopy, visible light optics, and medical imaging. Propagation based phase retrieval, where the phase is obtained from defocused images, has shown significant promise. There are, however, limitations in the accuracy of the retrieved phase arising from such methods. Sources of error include shot noise, image misalignment, and diffraction artifacts. We explore the use of artificial neural networks (ANNs) to improve the accuracy of propagation based phase retrieval algorithms applied to simulated intensity measurements. We employ a phase retrieval algorithm based on the transport-of-intensity equation to obtain the phase from simulated micrographs of procedurally generated specimens. We then train an ANN with pairs of retrieved and exact phases, and use the trained ANN to process a test set of retrieved phase maps. The total error in the phase is significantly reduced using this method. We also discuss a variety of potential extensions to this work.

Quantitative imaging with Fucci and mathematics to uncover temporal dynamics of cell cycle progression.

PubMed

Saitou, Takashi; Imamura, Takeshi

2016-01-01

Cell cycle progression is strictly coordinated to ensure proper tissue growth, development, and regeneration of multicellular organisms. Spatiotemporal visualization of cell cycle phases directly helps us to obtain a deeper understanding of controlled, multicellular, cell cycle progression. The fluorescent ubiquitination-based cell cycle indicator (Fucci) system allows us to monitor, in living cells, the G1 and the S/G2/M phases of the cell cycle in red and green fluorescent colors, respectively. Since the discovery of Fucci technology, it has found numerous applications in the characterization of the timing of cell cycle phase transitions under diverse conditions and various biological processes. However, due to the complexity of cell cycle dynamics, understanding of specific patterns of cell cycle progression is still far from complete. In order to tackle this issue, quantitative approaches combined with mathematical modeling seem to be essential. Here, we review several studies that attempted to integrate Fucci technology and mathematical models to obtain quantitative information regarding cell cycle regulatory patterns. Focusing on the technological development of utilizing mathematics to retrieve meaningful information from the Fucci producing data, we discuss how the combined methods advance a quantitative understanding of cell cycle regulation. © 2015 Japanese Society of Developmental Biologists.

A novel phase retrieval method from three-wavelength in-line phase-shifting interferograms based on positive negative 2π phase shifts

NASA Astrophysics Data System (ADS)

Xu, Xiaoqing; Wang, Yawei; Ji, Ying; Xu, Yuanyuan; Xie, Ming

2018-01-01

A new method to extract quantitative phases for each wavelength from three-wavelength in-line phase-shifting interferograms is proposed. Firstly, seven interferograms with positive negative 2π phase shifts are sequentially captured by using the phase-shifting technique. Secondly, six dc-term suppressed intensities can be achieved by the use of the algebraic algorithm. Finally, the wrapped phases at the three wavelengths can be acquired simultaneously from these six interferograms add-subtracting by employing the trigonometric function method. The surface morphology with increased ambiguity-free range at synthetic beat wavelength can be obtained, while maintaining the low noise precision of the single wavelength measurement, by combining this method with three-wavelength phase unwrapping method. We illustrate the principle of this algorithm, and the simulated experiments of the spherical cap and the HeLa cell are conducted to prove our proposed method, respectively.

Ultra-fast quantitative imaging using ptychographic iterative engine based digital micro-mirror device

NASA Astrophysics Data System (ADS)

Sun, Aihui; Tian, Xiaolin; Kong, Yan; Jiang, Zhilong; Liu, Fei; Xue, Liang; Wang, Shouyu; Liu, Cheng

2018-01-01

As a lensfree imaging technique, ptychographic iterative engine (PIE) method can provide both quantitative sample amplitude and phase distributions avoiding aberration. However, it requires field of view (FoV) scanning often relying on mechanical translation, which not only slows down measuring speed, but also introduces mechanical errors decreasing both resolution and accuracy in retrieved information. In order to achieve high-accurate quantitative imaging with fast speed, digital micromirror device (DMD) is adopted in PIE for large FoV scanning controlled by on/off state coding by DMD. Measurements were implemented using biological samples as well as USAF resolution target, proving high resolution in quantitative imaging using the proposed system. Considering its fast and accurate imaging capability, it is believed the DMD based PIE technique provides a potential solution for medical observation and measurements.

Low-dose quantitative phase contrast medical CT

NASA Astrophysics Data System (ADS)

Mittone, A.; Bravin, A.; Coan, P.

2018-02-01

X-ray computed tomography (CT) is a powerful and routinely used clinical diagnostic technique, which is well tolerated by patients, and which provides high-resolution images and volumetric information about the body. However, two important limitations still affect this examination procedure: (1) its low sensitivity with respect to soft tissues, and (2) the hazards associated with x-ray exposure. Conventional radiology is based on the detection of the different photon absorption properties that characterize biological tissues, and thus the obtainable image contrast from soft and/or similar tissues is intrinsically limited. In this scenario, x-ray phase contrast imaging (XPCI) has been extensively tested and proven to overcome some of the main issues surrounding standard x-ray imaging. In addition to the absorption signal, XPCI relies on detecting the phase shifts induced by an object. Interestingly, as the order of magnitude of the phase contrast is higher than that of absorption, XPCI can, in principle, offer higher sensitivity at lower radiation doses. However, other technical aspects may counterbalance this gain, and an optimized setup and image processing solutions need to be implemented. The work presented here describes the strategies and developments we have realized, with the aim of controlling the radiation dose for the highly sensitive and quantitative XPCI-CT. Different algorithms for the phase retrieval and CT reconstruction of the XPCI data are presented. The CT algorithms we have implemented, namely the equally sloped tomography and the dictionary learning method, allow the image quality to be preserved while reducing the number of angular projections required by a factor of five. The results applied to breast imaging report accurate reconstructions at clinically compatible doses of the 3D distribution of the refractive properties of full human organs obtained by using three different phase retrieval methods. The described methodologies and the presented results have been validated by a team of clinical radiologists and represent an important step in the exploitation of XPCI-CT for in vivo and possible clinical applications.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Claus, Rene A.; Wang, Yow-Gwo; Wojdyla, Antoine

Extreme Ultraviolet (EUV) Lithography mask defects were examined on the actinic mask imaging system, SHARP, at Lawrence Berkeley National Laboratory. Also, a quantitative phase retrieval algorithm based on the Weak Object Transfer Function was applied to the measured through-focus aerial images to examine the amplitude and phase of the defects. The accuracy of the algorithm was demonstrated by comparing the results of measurements using a phase contrast zone plate and a standard zone plate. Using partially coherent illumination to measure frequencies that would otherwise fall outside the numerical aperture (NA), it was shown that some defects are smaller than themore » conventional resolution of the microscope. We found that the programmed defects of various sizes were measured and shown to have both an amplitude and a phase component that the algorithm is able to recover.« less

Phase measurements of EUV mask defects

DOE PAGES

Claus, Rene A.; Wang, Yow-Gwo; Wojdyla, Antoine; ...

2015-02-22

Extreme Ultraviolet (EUV) Lithography mask defects were examined on the actinic mask imaging system, SHARP, at Lawrence Berkeley National Laboratory. Also, a quantitative phase retrieval algorithm based on the Weak Object Transfer Function was applied to the measured through-focus aerial images to examine the amplitude and phase of the defects. The accuracy of the algorithm was demonstrated by comparing the results of measurements using a phase contrast zone plate and a standard zone plate. Using partially coherent illumination to measure frequencies that would otherwise fall outside the numerical aperture (NA), it was shown that some defects are smaller than themore » conventional resolution of the microscope. We found that the programmed defects of various sizes were measured and shown to have both an amplitude and a phase component that the algorithm is able to recover.« less

Microphysical Properties of Alaskan Volcanic Ash

NASA Astrophysics Data System (ADS)

Puthukkudy, A.; Espinosa, R.; Rocha Lima, A.; Remer, L.; Colarco, P. R.; Whelley, P.; Krotkov, N. A.; Young, K.; Dubovik, O.; Wallace, K.; Martins, J. V.

2017-12-01

Volcanic ash has the potential to cause a variety of severe problems for human health and the environment. Therefore, effective monitoring of the dispersion and fallout from volcanic ash clouds and characterization of the aerosol particle properties are essential. One way to acquire information from volcanic clouds is through satellite remote sensing: such images have greater coverage than ground-based observations and can present a "big picture" perspective. A challenge of remote sensing is that assumptions of certain properties of the target are often a pre-requisite for making accurate and quantitative retrievals. For example, detailed information about size distribution, sphericity, and optical properties of the constituent matter is needed or must be assumed. The same kind of information is also needed for atmospheric transport models to properly simulate the dispersion and fallout of volcanic ash. Presented here is a laboratory method to determine the microphysical and optical properties of volcanic ash samples collected from two Alaskan volcanoes with markedly different compositions. Our method uses a Polarized Imaging Nephelometer (PI-Neph) and a system that re-suspends the particles in an air flow. The PI-Neph measures angular light scattering and polarization of the re-suspended particles from 3o to 175o in scattering angle, with an angular resolution of 1o . Primary measurements include phase function and polarized phase function at three wavelengths (445nm, 532nm, and 661nm). Size distribution, sphericity, and complex refractive index are retrieved indirectly from the PI-Neph measurements using the GRASP (Generalized Retrieval of Aerosol and Surface Properties) inversion algorithm. We report the results of this method applied to samples from the Mt. Okmok (2008) and Mt. Katmai (1912) volcanic eruptions. To our knowledge, this is the first time direct measurements of phase matrix elements of ash from Mt. Okmok and Mt. Katmai have been reported. Retrieved microphysical properties show that Mt. Katmai ash is less absorbing than the Mt. Okmok ash in visible wavelengths. Phase function of these Alaskan volcanic ashes is smooth curve without any significant features. Phase function and polarized phase function measured do not exhibit strong spectral dependence in visible wavelengths.

Status on Iterative Transform Phase Retrieval Applied to the GBT Data

NASA Technical Reports Server (NTRS)

Dean, Bruce; Aronstein, David; Smith, Scott; Shiri, Ron; Hollis, Jan M.; Lyons, Richard; Prestage, Richard; Hunter, Todd; Ghigo, Frank; Nikolic, Bojan

2007-01-01

This slide presentation reviews the use of iterative transform phase retrieval in the analysis of the Green Bank Radio Telescope (GBT) Data. It reviews the NASA projects that have used phase retrieval, and the testbed for the algorithm to be used for the James Webb Space Telescope. It shows the comparison of phase retrieval with an interferometer, and reviews the two approaches used for phase retrieval, iterative transform (ITA) or parametric (non-linear least squares model fitting). The concept of ITA Phase Retrieval is reviewed, and the application to Radio Antennas is reviewed. The presentation also examines the National Radio Astronomy Observatory (NRAO) data from the GBT, and the Fourier model that NRAO uses to analyze the data. The challenge for ITA phase retrieval is reviewed, and the coherent approximation for incoherent data is shown. The validity of the approximation is good for a large tilt. There is a review of the proof of concept of the Phase Review simulation using the input wavefront, and the initial sampling parameters estimate from the focused GBT data.

Broadband Phase Retrieval for Image-Based Wavefront Sensing

NASA Technical Reports Server (NTRS)

Dean, Bruce H.

2007-01-01

A focus-diverse phase-retrieval algorithm has been shown to perform adequately for the purpose of image-based wavefront sensing when (1) broadband light (typically spanning the visible spectrum) is used in forming the images by use of an optical system under test and (2) the assumption of monochromaticity is applied to the broadband image data. Heretofore, it had been assumed that in order to obtain adequate performance, it is necessary to use narrowband or monochromatic light. Some background information, including definitions of terms and a brief description of pertinent aspects of image-based phase retrieval, is prerequisite to a meaningful summary of the present development. Phase retrieval is a general term used in optics to denote estimation of optical imperfections or aberrations of an optical system under test. The term image-based wavefront sensing refers to a general class of algorithms that recover optical phase information, and phase-retrieval algorithms constitute a subset of this class. In phase retrieval, one utilizes the measured response of the optical system under test to produce a phase estimate. The optical response of the system is defined as the image of a point-source object, which could be a star or a laboratory point source. The phase-retrieval problem is characterized as image-based in the sense that a charge-coupled-device camera, preferably of scientific imaging quality, is used to collect image data where the optical system would normally form an image. In a variant of phase retrieval, denoted phase-diverse phase retrieval [which can include focus-diverse phase retrieval (in which various defocus planes are used)], an additional known aberration (or an equivalent diversity function) is superimposed as an aid in estimating unknown aberrations by use of an image-based wavefront-sensing algorithm. Image-based phase-retrieval differs from such other wavefront-sensing methods, such as interferometry, shearing interferometry, curvature wavefront sensing, and Shack-Hartmann sensing, all of which entail disadvantages in comparison with image-based methods. The main disadvantages of these non-image based methods are complexity of test equipment and the need for a wavefront reference.

Digital micromirror device as amplitude diffuser for multiple-plane phase retrieval

NASA Astrophysics Data System (ADS)

Abregana, Timothy Joseph T.; Hermosa, Nathaniel P.; Almoro, Percival F.

2017-06-01

Previous implementations of the phase diffuser used in the multiple-plane phase retrieval method included a diffuser glass plate with fixed optical properties or a programmable yet expensive spatial light modulator. Here a model for phase retrieval based on a digital micromirror device as amplitude diffuser is presented. The technique offers programmable, convenient and low-cost amplitude diffuser for a non-stagnating iterative phase retrieval. The technique is demonstrated in the reconstructions of smooth object wavefronts.

Phase retrieval using a modified Shack-Hartmann wavefront sensor with defocus.

PubMed

Li, Changwei; Li, Bangming; Zhang, Sijiong

2014-02-01

This paper proposes a modified Shack-Hartmann wavefront sensor for phase retrieval. The sensor is revamped by placing a detector at a defocused plane before the focal plane of the lenslet array of the Shack-Hartmann sensor. The algorithm for phase retrieval is an optimization with initial Zernike coefficients calculated by the conventional phase reconstruction of the Shack-Hartmann sensor. Numerical simulations show that the proposed sensor permits sensitive, accurate phase retrieval. Furthermore, experiments tested the feasibility of phase retrieval using the proposed sensor. The surface irregularity for a flat mirror was measured by the proposed method and a Veeco interferometer, respectively. The irregularity for the mirror measured by the proposed method is in very good agreement with that measured using the Veeco interferometer.

Quantification of signal detection performance degradation induced by phase-retrieval in propagation-based x-ray phase-contrast imaging

NASA Astrophysics Data System (ADS)

Chou, Cheng-Ying; Anastasio, Mark A.

2016-04-01

In propagation-based X-ray phase-contrast (PB XPC) imaging, the measured image contains a mixture of absorption- and phase-contrast. To obtain separate images of the projected absorption and phase (i.e., refractive) properties of a sample, phase retrieval methods can be employed. It has been suggested that phase-retrieval can always improve image quality in PB XPC imaging. However, when objective (task-based) measures of image quality are employed, this is not necessarily true and phase retrieval can be detrimental. In this work, signal detection theory is utilized to quantify the performance of a Hotelling observer (HO) for detecting a known signal in a known background. Two cases are considered. In the first case, the HO acts directly on the measured intensity data. In the second case, the HO acts on either the retrieved phase or absorption image. We demonstrate that the performance of the HO is superior when acting on the measured intensity data. The loss of task-specific information induced by phase-retrieval is quantified by computing the efficiency of the HO as the ratio of the test statistic signal-to-noise ratio (SNR) for the two cases. The effect of the system geometry on this efficiency is systematically investigated. Our findings confirm that phase-retrieval can impair signal detection performance in XPC imaging.

Digital holographic microscopy for toxicity testing and cell culture quality control

NASA Astrophysics Data System (ADS)

Kemper, Björn

2018-02-01

For the example of digital holographic microscopy (DHM), it is illustrated how label-free biophysical parameter sets can be extracted from quantitative phase images of adherent and suspended cells, and how the retrieved data can be applied for in-vitro toxicity testing and cell culture quality assessment. This includes results from the quantification of the reactions of cells to toxic substances as well as data from sophisticated monitoring of cell alterations that are related to changes of cell culture conditions.

Digital holographic microscopy combined with optical tweezers

NASA Astrophysics Data System (ADS)

Cardenas, Nelson; Yu, Lingfeng; Mohanty, Samarendra K.

2011-02-01

While optical tweezers have been widely used for the manipulation and organization of microscopic objects in three dimensions, observing the manipulated objects along axial direction has been quite challenging. In order to visualize organization and orientation of objects along axial direction, we report development of a Digital holographic microscopy combined with optical tweezers. Digital holography is achieved by use of a modified Mach-Zehnder interferometer with digital recording of interference pattern of the reference and sample laser beams by use of a single CCD camera. In this method, quantitative phase information is retrieved dynamically with high temporal resolution, only limited by frame rate of the CCD. Digital focusing, phase-unwrapping as well as online analysis and display of the quantitative phase images was performed on a software developed on LabView platform. Since phase changes observed in DHOT is very sensitive to optical thickness of trapped volume, estimation of number of particles trapped in the axial direction as well as orientation of non-spherical objects could be achieved with high precision. Since in diseases such as malaria and diabetics, change in refractive index of red blood cells occurs, this system can be employed to map such disease-specific changes in biological samples upon immobilization with optical tweezers.

Phase Retrieval on Undersampled Data from the Thermal Infrared Sensor (TIRS)

NASA Technical Reports Server (NTRS)

Bolcar, Matthew R.; Mentzell, Eric

2011-01-01

Phase retrieval was applied to under-sampled data from a thermal infrared imaging system to estimate defocus across the field of view (FOV). We compare phase retrieval estimated values to those obtained using an independent technique.

An Orbital "Virtual Radar" from TRMM Passive Microwave and Lightning Observations

NASA Technical Reports Server (NTRS)

Boccippio, Dennis J.

2004-01-01

The retrieval of vertical structure from joint passive microwave and lightning observations is demonstrated. Three years of data from the TRMM (Tropical Rainfall Measuring Mission) are used as a training dataset for regression and classification neural networks; the TMI (TRMM Microwave Imager) and LIS (Lightning Imaging Sensor) provide the inputs, the PR (Precipitation Radar) provides the training targets. Both vertical reflectivity profile categorization (into 9 convective, 7 stratiform, 2 mixed and 6 anvil types) and geophysical parameters (surface rainfall, vertically integrated liquid (VIL), ice water content (IWC) and echo tops) are retrieved. Retrievals are successful over both land and ocean surfaces. The benefit of using lightning observations as inputs to these retrievals is quantitatively demonstrated; lightning essentially provides an additional convective/stratiform discriminator, and is most important for isolation of midlevel (tops in the mixed phase region) convective profile types (this is because high frequency passive microwave observations already provide good convective/stratiform discrimination for deep convective profiles). This is highly relevant as midlevel convective profiles account for an extremely large fraction of tropical rainfall, and yet are most difficult to discriminate from comparable-depth stratiform profile types using passive microwave observations alone.

Complex amplitude reconstruction by iterative amplitude-phase retrieval algorithm with reference

NASA Astrophysics Data System (ADS)

Shen, Cheng; Guo, Cheng; Tan, Jiubin; Liu, Shutian; Liu, Zhengjun

2018-06-01

Multi-image iterative phase retrieval methods have been successfully applied in plenty of research fields due to their simple but efficient implementation. However, there is a mismatch between the measurement of the first long imaging distance and the sequential interval. In this paper, an amplitude-phase retrieval algorithm with reference is put forward without additional measurements or priori knowledge. It gets rid of measuring the first imaging distance. With a designed update formula, it significantly raises the convergence speed and the reconstruction fidelity, especially in phase retrieval. Its superiority over the original amplitude-phase retrieval (APR) method is validated by numerical analysis and experiments. Furthermore, it provides a conceptual design of a compact holographic image sensor, which can achieve numerical refocusing easily.

A peptide-retrieval strategy enables significant improvement of quantitative performance without compromising confidence of identification.

PubMed

Tu, Chengjian; Shen, Shichen; Sheng, Quanhu; Shyr, Yu; Qu, Jun

2017-01-30

Reliable quantification of low-abundance proteins in complex proteomes is challenging largely owing to the limited number of spectra/peptides identified. In this study we developed a straightforward method to improve the quantitative accuracy and precision of proteins by strategically retrieving the less confident peptides that were previously filtered out using the standard target-decoy search strategy. The filtered-out MS/MS spectra matched to confidently-identified proteins were recovered, and the peptide-spectrum-match FDR were re-calculated and controlled at a confident level of FDR≤1%, while protein FDR maintained at ~1%. We evaluated the performance of this strategy in both spectral count- and ion current-based methods. >60% increase of total quantified spectra/peptides was respectively achieved for analyzing a spike-in sample set and a public dataset from CPTAC. Incorporating the peptide retrieval strategy significantly improved the quantitative accuracy and precision, especially for low-abundance proteins (e.g. one-hit proteins). Moreover, the capacity of confidently discovering significantly-altered proteins was also enhanced substantially, as demonstrated with two spike-in datasets. In summary, improved quantitative performance was achieved by this peptide recovery strategy without compromising confidence of protein identification, which can be readily implemented in a broad range of quantitative proteomics techniques including label-free or labeling approaches. We hypothesize that more quantifiable spectra and peptides in a protein, even including less confident peptides, could help reduce variations and improve protein quantification. Hence the peptide retrieval strategy was developed and evaluated in two spike-in sample sets with different LC-MS/MS variations using both MS1- and MS2-based quantitative approach. The list of confidently identified proteins using the standard target-decoy search strategy was fixed and more spectra/peptides with less confidence matched to confident proteins were retrieved. However, the total peptide-spectrum-match false discovery rate (PSM FDR) after retrieval analysis was still controlled at a confident level of FDR≤1%. As expected, the penalty for occasionally incorporating incorrect peptide identifications is negligible by comparison with the improvements in quantitative performance. More quantifiable peptides, lower missing value rate, better quantitative accuracy and precision were significantly achieved for the same protein identifications by this simple strategy. This strategy is theoretically applicable for any quantitative approaches in proteomics and thereby provides more quantitative information, especially on low-abundance proteins. Published by Elsevier B.V.

High speed phase retrieval of in-line holograms by the assistance of corresponding off-axis holograms.

PubMed

Orzó, László

2015-06-29

Retrieving correct phase information from an in-line hologram is difficult as the object wave field and the diffractions of the zero order and the conjugate object term overlap. The existing iterative numerical phase retrieval methods are slow, especially in the case of high Fresnel number systems. Conversely, the reconstruction of the object wave field from an off-axis hologram is simple, but due to the applied spatial frequency filtering the achievable resolution is confined. Here, a new, high-speed algorithm is introduced that efficiently incorporates the data of an auxiliary off-axis hologram in the phase retrieval of the corresponding in-line hologram. The efficiency of the introduced combined phase retrieval method is demonstrated by simulated and measured holograms.

Phase modulated high density collinear holographic data storage system with phase-retrieval reference beam locking and orthogonal reference encoding.

PubMed

Liu, Jinpeng; Horimai, Hideyoshi; Lin, Xiao; Huang, Yong; Tan, Xiaodi

2018-02-19

A novel phase modulation method for holographic data storage with phase-retrieval reference beam locking is proposed and incorporated into an amplitude-encoding collinear holographic storage system. Unlike the conventional phase retrieval method, the proposed method locks the data page and the corresponding phase-retrieval interference beam together at the same location with a sequential recording process, which eliminates piezoelectric elements, phase shift arrays and extra interference beams, making the system more compact and phase retrieval easier. To evaluate our proposed phase modulation method, we recorded and then recovered data pages with multilevel phase modulation using two spatial light modulators experimentally. For 4-level, 8-level, and 16-level phase modulation, we achieved the bit error rate (BER) of 0.3%, 1.5% and 6.6% respectively. To further improve data storage density, an orthogonal reference encoding multiplexing method at the same position of medium is also proposed and validated experimentally. We increased the code rate of pure 3/16 amplitude encoding method from 0.5 up to 1.0 and 1.5 using 4-level and 8-level phase modulation respectively.

A new 3D tracking method for cell mechanics investigation exploiting the capabilities of digital holography in microscopy

NASA Astrophysics Data System (ADS)

Miccio, L.; Memmolo, P.; Merola, F.; Fusco, S.; Netti, P. A.; Ferraro, P.

2014-03-01

A method for 3D tracking has been developed exploiting Digital Holography features in Microscopy (DHM). In the framework of self-consistent platform for manipulation and measurement of biological specimen we use DHM for quantitative and completely label free analysis of samples with low amplitude contrast. Tracking capability extend the potentiality of DHM allowing to monitor the motion of appropriate probes and correlate it with sample properties. Complete 3D tracking has been obtained for the probes avoiding the amplitude refocusing in traditional tracking processes. Moreover, in biology and biomedical research fields one of the main topic is the understanding of morphology and mechanics of cells and microorganisms. Biological samples present low amplitude contrast that limits the information that can be retrieved through optical bright-field microscope measurements. The main effect on light propagating in such objects is in phase. This is known as phase-retardation or phase-shift. DHM is an innovative and alternative approach in microscopy, it's a good candidate for no-invasive and complete specimen analysis because its main characteristic is the possibility to discern between intensity and phase information performing quantitative mapping of the Optical Path Length. In this paper, the flexibility of DH is employed to analyze cell mechanics of unstained cells subjected to appropriate stimuli. DHM is used to measure all the parameters useful to understand the deformations induced by external and controlled stresses on in-vitro cells. Our configuration allows 3D tracking of micro-particles and, simultaneously, furnish quantitative phase-contrast maps. Experimental results are presented and discussed for in vitro cells.

Phase and Pupil Amplitude Recovery for JWST Space-Optics Control

NASA Technical Reports Server (NTRS)

Dean, B. H.; Zielinski, T. P.; Smith, J. S.; Bolcar, M. R.; Aronstein, D. L.; Fienup, J. R.

2010-01-01

This slide presentation reviews the phase and pupil amplitude recovery for the James Webb Space Telescope (JWST) Near Infrared Camera (NIRCam). It includes views of the Integrated Science Instrument Module (ISIM), the NIRCam, examples of Phase Retrieval Data, Ghost Irradiance, Pupil Amplitude Estimation, Amplitude Retrieval, Initial Plate Scale Estimation using the Modulation Transfer Function (MTF), Pupil Amplitude Estimation vs lambda, Pupil Amplitude Estimation vs. number of Images, Pupil Amplitude Estimation vs Rotation (clocking), and Typical Phase Retrieval Results Also included is information about the phase retrieval approach, Non-Linear Optimization (NLO) Optimized Diversity Functions, and Least Square Error vs. Starting Pupil Amplitude.

In Vitro versus In Vivo Phase Instability of Zirconia-Toughened Alumina Femoral Heads: A Critical Comparative Assessment

PubMed Central

Pezzotti, Giuseppe; Affatato, Saverio; Rondinella, Alfredo; Yorifuji, Makiko; Marin, Elia; Zhu, Wenliang; McEntire, Bryan; Bal, Sonny B.; Yamamoto, Kengo

2017-01-01

A clear discrepancy between predicted in vitro and actual in vivo surface phase stability of BIOLOX®delta zirconia-toughened alumina (ZTA) femoral heads has been demonstrated by several independent research groups. Data from retrievals challenge the validity of the standard method currently utilized in evaluating surface stability and raise a series of important questions: (1) Why do in vitro hydrothermal aging treatments conspicuously fail to model actual results from the in vivo environment? (2) What is the preponderant microscopic phenomenon triggering the accelerated transformation in vivo? (3) Ultimately, what revisions of the current in vitro standard are needed in order to obtain consistent predictions of ZTA transformation kinetics in vivo? Reported in this paper is a new in toto method for visualizing the surface stability of femoral heads. It is based on CAD-assisted Raman spectroscopy to quantitatively assess the phase transformation observed in ZTA retrievals. Using a series of independent analytical probes, an evaluation of the microscopic mechanisms responsible for the polymorphic transformation is also provided. An outline is given of the possible ways in which the current hydrothermal simulation standard for artificial joints can be improved in an attempt to reduce the gap between in vitro simulation and reality. PMID:28772828

Genetic Algorithm Phase Retrieval for the Systematic Image-Based Optical Alignment Testbed

NASA Technical Reports Server (NTRS)

Taylor, Jaime; Rakoczy, John; Steincamp, James

2003-01-01

Phase retrieval requires calculation of the real-valued phase of the pupil fimction from the image intensity distribution and characteristics of an optical system. Genetic 'algorithms were used to solve two one-dimensional phase retrieval problem. A GA successfully estimated the coefficients of a polynomial expansion of the phase when the number of coefficients was correctly specified. A GA also successfully estimated the multiple p h e s of a segmented optical system analogous to the seven-mirror Systematic Image-Based Optical Alignment (SIBOA) testbed located at NASA s Marshall Space Flight Center. The SIBOA testbed was developed to investigate phase retrieval techniques. Tiphilt and piston motions of the mirrors accomplish phase corrections. A constant phase over each mirror can be achieved by an independent tip/tilt correction: the phase Conection term can then be factored out of the Discrete Fourier Tranform (DFT), greatly reducing computations.

Tissue refractometry using Hilbert phase microscopy.

PubMed

Lue, Niyom; Bewersdorf, Joerg; Lessard, Mark D; Badizadegan, Kamran; Dasari, Ramachandra R; Feld, Michael S; Popescu, Gabriel

2007-12-15

We present, for the first time to our knowledge, quantitative phase images associated with unstained 5 mum thick tissue slices of mouse brain, spleen, and liver. The refractive properties of the tissue are retrieved in terms of the average refractive index and its spatial variation. We find that the average refractive index varies significantly with tissue type, such that the brain is characterized by the lowest value and the liver by the highest. The spatial power spectra of the phase images reveal power law behavior with different exponents for each tissue type. This approach opens a new possibility for stain-free characterization of tissues, where the diagnostic power is provided by the intrinsic refractive properties of the biological structure. We present results obtained for liver tissue affected by a lysosomal storage disease and show that our technique can quantify structural changes during this disease development.

Lensless transport-of-intensity phase microscopy and tomography with a color LED matrix

NASA Astrophysics Data System (ADS)

Zuo, Chao; Sun, Jiasong; Zhang, Jialin; Hu, Yan; Chen, Qian

2015-07-01

We demonstrate lens-less quantitative phase microscopy and diffraction tomography based on a compact on-chip platform, using only a CMOS image sensor and a programmable color LED array. Based on multi-wavelength transport-of- intensity phase retrieval and multi-angle illumination diffraction tomography, this platform offers high quality, depth resolved images with a lateral resolution of ˜3.7μm and an axial resolution of ˜5μm, over wide large imaging FOV of 24mm2. The resolution and FOV can be further improved by using a larger image sensors with small pixels straightforwardly. This compact, low-cost, robust, portable platform with a decent imaging performance may offer a cost-effective tool for telemedicine needs, or for reducing health care costs for point-of-care diagnostics in resource-limited environments.

Broadband standoff detection of large molecules by mid-infrared active coherent laser spectrometry.

PubMed

Macleod, Neil A; Molero, Francisco; Weidmann, Damien

2015-01-26

A widely tunable active coherent laser spectrometer (ACLaS) has been demonstrated for standoff detection of broadband absorbers in the 1280 to 1318 cm^-1 spectral region using an external cavity quantum cascade laser as a mid-infrared source. The broad tuning range allows detection and quantification of vapor phase molecules, such as dichloroethane, ethylene glycol dinitrate, and tetrafluoroethane. The level of confidence in molecular mixing ratios retrieved from interfering spectral measurements is assessed in a quantitative manner. A first qualitative demonstration of condensed phase chemical detection on nitroacetanilide has also been conducted. Detection performances of the broadband ACLaS have been placed in the context of explosive detection and compared to that obtained using distributed feedback quantum cascade lasers.

Quantitative Comparison of the Walk and Trot of Border Collies and Labrador Retrievers, Breeds with Different Performance Requirements

PubMed Central

Carr, Brittany Jean; Canapp, Sherman O; Zink, M. Christine

2015-01-01

Introduction We hypothesized that breed differences of Border Collies and Labrador Retrievers would be reflected in the temporospatial characteristics of the walk and trot. Materials and Methods Twenty healthy Border Collies and 20 healthy Labrador Retrievers made three passes across a pressure sensing walkway system that recorded quantitative temporospatial information at a walk and a trot. The following variables were measured for each dog: velocity, total pressure index percentage (TPI%), ratio of weight borne on the thoracic vs. pelvic limbs (T/P TPI%), stance time percentage (ST%), and thoracic limb stride length (TSrL). Results The mean T/P TPI% for Border Collies at a walk and at a trot were significantly lower than for Labrador Retrievers (p = 0.0007 and p = 0.0003). Border Collies had a significantly lower ST% than Labrador Retrievers for the thoracic limbs and pelvic limbs at a walk (p = 0.0058 and 0.0003) and the trot (p = 0.0280 and 0.0448). There was no relationship between ST% and TSrL in Border Collies and an inverse correlation between ST% and TSrL in Labrador Retrievers (p = 0.0002). Discussion Key quantitative gait differences were identified in Border Collies and Labrador Retrievers, which could potentially provide each breed with an advantage for their working function. PMID:26689372

Optical image transformation and encryption by phase-retrieval-based double random-phase encoding and compressive ghost imaging

NASA Astrophysics Data System (ADS)

Yuan, Sheng; Yang, Yangrui; Liu, Xuemei; Zhou, Xin; Wei, Zhenzhuo

2018-01-01

An optical image transformation and encryption scheme is proposed based on double random-phase encoding (DRPE) and compressive ghost imaging (CGI) techniques. In this scheme, a secret image is first transformed into a binary image with the phase-retrieval-based DRPE technique, and then encoded by a series of random amplitude patterns according to the ghost imaging (GI) principle. Compressive sensing, corrosion and expansion operations are implemented to retrieve the secret image in the decryption process. This encryption scheme takes the advantage of complementary capabilities offered by the phase-retrieval-based DRPE and GI-based encryption techniques. That is the phase-retrieval-based DRPE is used to overcome the blurring defect of the decrypted image in the GI-based encryption, and the CGI not only reduces the data amount of the ciphertext, but also enhances the security of DRPE. Computer simulation results are presented to verify the performance of the proposed encryption scheme.

Retrieval of Aerosol Phase Function and Polarized Phase Function from Polarization of Skylight for Different Observation Geometries

NASA Astrophysics Data System (ADS)

Li, L.; Qie, L. L.; Xu, H.; Li, Z. Q.

2018-04-01

The phase function and polarized phase function are important optical parameters, which describe scattering properties of atmospheric aerosol particles. Polarization of skylight induced by the scattering processes is sensitive to the scattering properties of aerosols. The Stokes parameters I, Q, U and the polarized radiance Lp of skylight measured by the CIMEL dual-polar sun-sky radiometer CE318- DP can be use to retrieve the phase function and polarized phase function, respectively. Two different observation geometries (i.e., the principal plane and almucantar) are preformed by the CE318-DP to detect skylight polarization. Polarization of skylight depends on the illumination and observation geometries. For the same solar zenith angle, retrievals of the phase function and the polarized phase function are still affected by the observation geometry. The performance of the retrieval algorithm for the principal plane and almucantar observation geometries was assessed by the numerical experiments at two typical high and low sun's positions (i.e. solar zenith angles are equal to 45° and 65°). Comparing the results for the principal plane and almucantar geometries, it is recommended to utilize the principal plane observations to retrieve the phase function when the solar zenith angle is small. The Stokes parameter U and the polarized radiance Lp from the almucantar observations are suggested to retrieve the polarized phase function, especially for short wavelength channels (e.g., 440 and 500 nm).

An introduction to the theory of ptychographic phase retrieval methods

NASA Astrophysics Data System (ADS)

Konijnenberg, Sander

2017-12-01

An overview of several ptychographic phase retrieval methods and the theory behind them is presented. By looking into the theory behind more basic single-intensity pattern phase retrieval methods, a theoretical framework is provided for analyzing ptychographic algorithms. Extensions of ptychographic algorithms that deal with issues such as partial coherence, thick samples, or uncertainties of the probe or probe positions are also discussed. This introduction is intended for scientists and students without prior experience in the field of phase retrieval or ptychography to quickly get introduced to the theory, so that they can put the more specialized literature in context more easily.

Phase-step retrieval for tunable phase-shifting algorithms

NASA Astrophysics Data System (ADS)

Ayubi, Gastón A.; Duarte, Ignacio; Perciante, César D.; Flores, Jorge L.; Ferrari, José A.

2017-12-01

Phase-shifting (PS) is a well-known technique for phase retrieval in interferometry, with applications in deflectometry and 3D-profiling, which requires a series of intensity measurements with certain phase-steps. Usually the phase-steps are evenly spaced, and its knowledge is crucial for the phase retrieval. In this work we present a method to extract the phase-step between consecutive interferograms. We test the proposed technique with images corrupted by additive noise. The results were compared with other known methods. We also present experimental results showing the performance of the method when spatial filters are applied to the interferograms and the effect that they have on their relative phase-steps.

Acute stress negatively affects object recognition early memory consolidation and memory retrieval unrelated to state-dependency.

PubMed

Nelissen, Ellis; Prickaerts, Jos; Blokland, Arjan

2018-06-01

It is well known that stress affects memory performance. However, there still appears to be inconstancy in literature about how acute stress affects the different stages of memory: acquisition, consolidation and retrieval. In this study, we exposed rats to acute stress and measured the effect on memory performance in the object recognition task as a measure for episodic memory. Stress was induced 30 min prior to the learning phase to affect acquisition, directly after the learning phase to affect consolidation, or 30 min before the retrieval phase to affect retrieval. Additionally, we induced stress both 30 min prior to the learning phase and 30 min prior to the retrieval phase to test whether the effects were related to state-dependency. As expected, we found that acute stress did not affect acquisition but had a negative impact on retrieval. To our knowledge, we are the first to show that early consolidation was negatively affected by acute stress. We also show that stress does not have a state-dependent effect on memory. Copyright © 2018 Elsevier B.V. All rights reserved.

Talbot-Lau x-ray deflectometry phase-retrieval methods for electron density diagnostics in high-energy density experiments.

PubMed

Valdivia, Maria Pia; Stutman, Dan; Stoeckl, Christian; Mileham, Chad; Begishev, Ildar A; Bromage, Jake; Regan, Sean P

2018-01-10

Talbot-Lau x-ray interferometry uses incoherent x-ray sources to measure refraction index changes in matter. These measurements can provide accurate electron density mapping through phase retrieval. An adaptation of the interferometer has been developed in order to meet the specific requirements of high-energy density experiments. This adaptation is known as a moiré deflectometer, which allows for single-shot capabilities in the form of interferometric fringe patterns. The moiré x-ray deflectometry technique requires a set of object and reference images in order to provide electron density maps, which can be costly in the high-energy density environment. In particular, synthetic reference phase images obtained ex situ through a phase-scan procedure, can provide a feasible solution. To test this procedure, an object phase map was retrieved from a single-shot moiré image obtained from a plasma-produced x-ray source. A reference phase map was then obtained from phase-stepping measurements using a continuous x-ray tube source in a small laboratory setting. The two phase maps were used to retrieve an electron density map. A comparison of the moiré and phase-stepping phase-retrieval methods was performed to evaluate single-exposure plasma electron density mapping for high-energy density and other transient plasma experiments. It was found that a combination of phase-retrieval methods can deliver accurate refraction angle mapping. Once x-ray backlighter quality is optimized, the ex situ method is expected to deliver electron density mapping with improved resolution. The steps necessary for improved diagnostic performance are discussed.

Transportable and vibration-free full-field low-coherent quantitative phase microscope

NASA Astrophysics Data System (ADS)

Yamauchi, Toyohiko; Yamada, Hidenao; Goto, Kentaro; Matsui, Hisayuki; Yasuhiko, Osamu; Ueda, Yukio

2018-02-01

We developed a transportable Linnik-type full-field low-coherent quantitative phase microscope that is able to compensate for optical path length (OPL) disturbance due to environmental mechanical noises. Though two-beam interferometers such as Linnik ones suffer from unstable OPL difference, we overcame this problem with a mechanical feedback system based on digital signal-processing that controls the OPL difference in sub-nanometer resolution precisely with a feedback bandwidth of 4 kHz. The developed setup has a footprint of 200 mm by 200 mm, a height of 500 mm, and a weight of 4.5 kilograms. In the transmission imaging mode, cells were cultured on a reflection-enhanced glass-bottom dish, and we obtained interference images sequentially while performing stepwise quarter-wavelength phase-shifting. Real-time image processing, including retrieval of the unwrapped phase from interference images and its background correction, along with the acquisition of interference images, was performed on a laptop computer. Emulation of the phase contrast (PhC) images and the differential interference contrast (DIC) images was also performed in real time. Moreover, our setup was applied for full-field cell membrane imaging in the reflection mode, where the cells were cultured on an anti-reflection (AR)-coated glass-bottom dish. The phase and intensity of the light reflected by the membrane revealed the outer shape of the cells independent of the refractive index. In this paper, we show imaging results on cultured cells in both transmission and reflection modes.

Phase retrieval of singular scalar light fields using a two-dimensional directional wavelet transform and a spatial carrier.

PubMed

Federico, Alejandro; Kaufmann, Guillermo H

2008-10-01

We evaluate a method based on the two-dimensional directional wavelet transform and the introduction of a spatial carrier to retrieve optical phase distributions in singular scalar light fields. The performance of the proposed phase-retrieval method is compared with an approach based on Fourier transform. The advantages and limitations of the proposed method are discussed.

Quantitative X-ray Differential Interference Contrast Microscopy

NASA Astrophysics Data System (ADS)

Nakamura, Takashi

Full-field soft x-ray microscopes are widely used in many fields of sciences. Advances in nanofabrication technology enabled short wavelength focusing elements with significantly improved spatial resolution. In the soft x-ray spectral region, samples as small as 12 nm can be resolved using micro zone-plates as the objective lens. In addition to conventional x-ray microscopy in which x-ray absorption difference provides the image contrast, phase contrast mechanisms such as differential phase contrast (DIC) and Zernike phase contrast have also been demonstrated These phase contrast imaging mechanisms are especially attractive at the x-ray wavelengths where phase contrast of most materials is typically 10 times stronger than the absorption contrast. With recent progresses in plasma-based x- ray sources and increasing accessibility to synchrotron user facilities, x-ray microscopes are quickly becoming standard measurement equipment in the laboratory. To further the usefulness of x-ray DIC microscopy this thesis explicitly addresses three known issues with this imaging modality by introducing new techniques and devices First, as opposed to its visible-light counterpart, no quantitative phase imaging technique exists for x-ray DIC microscopy. To address this issue, two nanoscale x-ray quantitative phase imaging techniques, using exclusive OR (XOR) patterns and zone-plate doublets, respectively, are proposed. Unlike existing x-ray quantitative phase imaging techniques such as Talbot interferometry and ptychography, no dedicated experimental setups or stringent illumination coherence are needed for quantitative phase retrieval. Second, to the best of our knowledge, no quantitative performance characterization of DIC microscopy exists to date. Therefore the imaging system's response to sample's spatial frequency is not known In order to gain in-depth understanding of this imaging modality, performance of x-ray DIC microscopy is quantified using modulation transfer function. A new illumination apparatus required for the transfer function analysis under partially coherent illumination is also proposed. Such a characterization is essential for a proper selection of DIC optics for various transparent samples under study. Finally, optical elements used for x-ray DIC microscopy are highly absorptive and high brilliance x-ray sources such as synchrotrons are generally needed for image contrast. To extend the use of x-ray DIC microscopy to a wider variety of applications, a high efficiency large numerical aperture optical element consisting of high reflective Bragg reflectors is proposed. Using Bragg reflectors, which have 70% ˜99% reflectivity at extreme ultraviolet and soft x-rays for all angles of glancing incidence, the first order focusing efficiency is expected to increase by ˜ 8 times compared to that of a typical Fresnel zone-plate. This thesis contributes to current nanoscale x-ray phase contrast imaging research and provides new insights for biological, material, and magnetic sciences

Variable Sampling Mapping

NASA Technical Reports Server (NTRS)

Smith, Jeffrey, S.; Aronstein, David L.; Dean, Bruce H.; Lyon, Richard G.

2012-01-01

The performance of an optical system (for example, a telescope) is limited by the misalignments and manufacturing imperfections of the optical elements in the system. The impact of these misalignments and imperfections can be quantified by the phase variations imparted on light traveling through the system. Phase retrieval is a methodology for determining these variations. Phase retrieval uses images taken with the optical system and using a light source of known shape and characteristics. Unlike interferometric methods, which require an optical reference for comparison, and unlike Shack-Hartmann wavefront sensors that require special optical hardware at the optical system's exit pupil, phase retrieval is an in situ, image-based method for determining the phase variations of light at the system s exit pupil. Phase retrieval can be used both as an optical metrology tool (during fabrication of optical surfaces and assembly of optical systems) and as a sensor used in active, closed-loop control of an optical system, to optimize performance. One class of phase-retrieval algorithms is the iterative transform algorithm (ITA). ITAs estimate the phase variations by iteratively enforcing known constraints in the exit pupil and at the detector, determined from modeled or measured data. The Variable Sampling Mapping (VSM) technique is a new method for enforcing these constraints in ITAs. VSM is an open framework for addressing a wide range of issues that have previously been considered detrimental to high-accuracy phase retrieval, including undersampled images, broadband illumination, images taken at or near best focus, chromatic aberrations, jitter or vibration of the optical system or detector, and dead or noisy detector pixels. The VSM is a model-to-data mapping procedure. In VSM, fully sampled electric fields at multiple wavelengths are modeled inside the phase-retrieval algorithm, and then these fields are mapped to intensities on the light detector, using the properties of the detector and optical system, for comparison with measured data. Ultimately, this model-to-data mapping procedure enables a more robust and accurate way of incorporating the exit-pupil and image detector constraints, which are fundamental to the general class of ITA phase retrieval algorithms.

Three-dimensional propagation in near-field tomographic X-ray phase retrieval

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ruhlandt, Aike, E-mail: aruhlan@gwdg.de; Salditt, Tim

An extension of phase retrieval algorithms for near-field X-ray (propagation) imaging to three dimensions is presented, enhancing the quality of the reconstruction by exploiting previously unused three-dimensional consistency constraints. This paper presents an extension of phase retrieval algorithms for near-field X-ray (propagation) imaging to three dimensions, enhancing the quality of the reconstruction by exploiting previously unused three-dimensional consistency constraints. The approach is based on a novel three-dimensional propagator and is derived for the case of optically weak objects. It can be easily implemented in current phase retrieval architectures, is computationally efficient and reduces the need for restrictive prior assumptions, resultingmore » in superior reconstruction quality.« less

Halo-free Phase Contrast Microscopy

NASA Astrophysics Data System (ADS)

Nguyen, Tan H.; Kandel, Mikhail; Shakir, Haadi M.; Best-Popescu, Catherine; Arikkath, Jyothi; Do, Minh N.; Popescu, Gabriel

2017-03-01

We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data for reversing the halo artifact. Acquiring four independent intensity images, our approach first measures haloed phase maps of the sample. We solve for the halo-free sample transmission function by using a physical model of the image formation under partial spatial coherence. Using this halo-free sample transmission, we can numerically generate artifact-free PC images. Furthermore, this transmission can be further used to obtain quantitative information about the sample, e.g., the thickness with known refractive indices, dry mass of live cells during their cycles. We tested our hfPC method on various control samples, e.g., beads, pillars and validated its potential for biological investigation by imaging live HeLa cells, red blood cells, and neurons.

Tissue refractometry using Hilbert phase microscopy

PubMed Central

Lue, Niyom; Bewersdorf, Joerg; Lessard, Mark D.; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.; Popescu, Gabriel

2009-01-01

We present, for the first time to our knowledge, quantitative phase images associated with unstained 5 μm thick tissue slices of mouse brain, spleen, and liver. The refractive properties of the tissue are retrieved in terms of the average refractive index and its spatial variation. We find that the average refractive index varies significantly with tissue type, such that the brain is characterized by the lowest value and the liver by the highest. The spatial power spectra of the phase images reveal power law behavior with different exponents for each tissue type. This approach opens a new possibility for stain-free characterization of tissues, where the diagnostic power is provided by the intrinsic refractive properties of the biological structure. We present results obtained for liver tissue affected by a lysosomal storage disease and show that our technique can quantify structural changes during this disease development. PMID:18087529

Phase contrast X-ray microtomography of the Rhodnius prolixus head: Comparison of direct reconstruction and phase retrieval approach

NASA Astrophysics Data System (ADS)

Almeida, A. P.; Braz, D.; Nogueira, L. P.; Colaço, M. V.; Soares, J.; Cardoso, S. C.; Garcia, E. S.; Azambuja, P.; Gonzalez, M. S.; Mohammadi, S.; Tromba, G.; Barroso, R. C.

2014-02-01

We have used phase-contrast X-ray microtomography (PPC-μCT) to study the head of the blood-feeding bug, Rhodnius prolixus, which is one of the most important insect vector of Trypanosoma cruzi, ethiologic agent of Chagas disease in Latin America. Images reconstructed from phase-retrieved projections processed by ANKA phase are compared to those obtained through direct tomographic reconstruction of the flat-field-corrected transmission radiographs. It should be noted that the relative locations of the important morphological internal structures are observable with a precision that is difficult to obtain without the phase retrieval approach.

Blue intensity matters for cell cycle profiling in fluorescence DAPI-stained images.

PubMed

Ferro, Anabela; Mestre, Tânia; Carneiro, Patrícia; Sahumbaiev, Ivan; Seruca, Raquel; Sanches, João M

2017-05-01

In the past decades, there has been an amazing progress in the understanding of the molecular mechanisms of the cell cycle. This has been possible largely due to a better conceptualization of the cycle itself, but also as a consequence of technological advances. Herein, we propose a new fluorescence image-based framework targeted at the identification and segmentation of stained nuclei with the purpose to determine DNA content in distinct cell cycle stages. The method is based on discriminative features, such as total intensity and area, retrieved from in situ stained nuclei by fluorescence microscopy, allowing the determination of the cell cycle phase of both single and sub-population of cells. The analysis framework was built on a modified k-means clustering strategy and refined with a Gaussian mixture model classifier, which enabled the definition of highly accurate classification clusters corresponding to G1, S and G2 phases. Using the information retrieved from area and fluorescence total intensity, the modified k-means (k=3) cluster imaging framework classified 64.7% of the imaged nuclei, as being at G1 phase, 12.0% at G2 phase and 23.2% at S phase. Performance of the imaging framework was ascertained with normal murine mammary gland cells constitutively expressing the Fucci2 technology, exhibiting an overall sensitivity of 94.0%. Further, the results indicate that the imaging framework has a robust capacity to both identify a given DAPI-stained nucleus to its correct cell cycle phase, as well as to determine, with very high probability, true negatives. Importantly, this novel imaging approach is a non-disruptive method that allows an integrative and simultaneous quantitative analysis of molecular and morphological parameters, thus awarding the possibility of cell cycle profiling in cytological and histological samples.

Phase retrieval without unwrapping by single-shot dual-wavelength digital holography

NASA Astrophysics Data System (ADS)

Min, Junwei; Yao, Baoli; Zhou, Meiling; Guo, Rongli; Lei, Ming; Yang, Yanlong; Dan, Dan; Yan, Shaohui; Peng, Tong

2014-12-01

A phase retrieval method by using single-shot dual-wavelength digital holography is proposed. Each single wavelength hologram is extracted from the color CCD recorded hologram at one exposure, and the unwrapped phase image of object can be reconstructed directly. Different from the traditional multiple wavelength phase unwrapping techniques, any single complex wave-fronts at different wavelengths have no need to be calculated any more. Thus, the phase retrieval is computationally fast and straightforward, and the limitations on the total optical path difference are significantly relaxed. The practicability of the proposed method is demonstrated by both simulated and experimental results.

Multi-sensor measurements of mixed-phase clouds above Greenland

NASA Astrophysics Data System (ADS)

Stillwell, Robert A.; Shupe, Matthew D.; Thayer, Jeffrey P.; Neely, Ryan R.; Turner, David D.

2018-04-01

Liquid-only and mixed-phase clouds in the Arctic strongly affect the regional surface energy and ice mass budgets, yet much remains unknown about the nature of these clouds due to the lack of intensive measurements. Lidar measurements of these clouds are challenged by very large signal dynamic range, which makes even seemingly simple tasks, such as thermodynamic phase classification, difficult. This work focuses on a set of measurements made by the Clouds Aerosol Polarization and Backscatter Lidar at Summit, Greenland and its retrieval algorithms, which use both analog and photon counting as well as orthogonal and non-orthogonal polarization retrievals to extend dynamic range and improve overall measurement quality and quantity. Presented here is an algorithm for cloud parameter retrievals that leverages enhanced dynamic range retrievals to classify mixed-phase clouds. This best guess retrieval is compared to co-located instruments for validation.

Off-axis digital holographic camera for quantitative phase microscopy.

PubMed

Monemhaghdoust, Zahra; Montfort, Frédéric; Emery, Yves; Depeursinge, Christian; Moser, Christophe

2014-06-01

We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a single shot. A thick holographic grating filters the beam containing the sample information in two dimensions through diffraction. The filtered beam creates the reference arm of the interferometer. The spatial filtering method, based on the high angular selectivity of the thick grating, reduces the alignment sensitivity to angular displacements compared with pinhole based Fourier filtering. The addition of a thin holographic grating alters the coherence plane tilt introduced by the thick grating so as to create high-visibility interference over the entire field of view. The acquired full-field off-axis holograms are processed to retrieve the amplitude and phase information of the sample. The system produces phase images of cheek cells qualitatively similar to phase images extracted with a standard commercial DHM.

Experiments on sparsity assisted phase retrieval of phase objects

NASA Astrophysics Data System (ADS)

Gaur, Charu; Lochab, Priyanka; Khare, Kedar

2017-05-01

Iterative phase retrieval algorithms such as the Gerchberg-Saxton method and the Fienup hybrid input-output method are known to suffer from the twin image stagnation problem, particularly when the solution to be recovered is complex valued and has centrosymmetric support. Recently we showed that the twin image stagnation problem can be addressed using image sparsity ideas (Gaur et al 2015 J. Opt. Soc. Am. A 32 1922). In this work we test this sparsity assisted phase retrieval method with experimental single shot Fourier transform intensity data frames corresponding to phase objects displayed on a spatial light modulator. The standard iterative phase retrieval algorithms are combined with an image sparsity based penalty in an adaptive manner. Illustrations for both binary and continuous phase objects are provided. It is observed that image sparsity constraint has an important role to play in obtaining meaningful phase recovery without encountering the well-known stagnation problems. The results are valuable for enabling single shot coherent diffraction imaging of phase objects for applications involving illumination wavelengths over a wide range of electromagnetic spectrum.

Phase Retrieval Using a Genetic Algorithm on the Systematic Image-Based Optical Alignment Testbed

NASA Technical Reports Server (NTRS)

Taylor, Jaime R.

2003-01-01

NASA s Marshall Space Flight Center s Systematic Image-Based Optical Alignment (SIBOA) Testbed was developed to test phase retrieval algorithms and hardware techniques. Individuals working with the facility developed the idea of implementing phase retrieval by breaking the determination of the tip/tilt of each mirror apart from the piston motion (or translation) of each mirror. Presented in this report is an algorithm that determines the optimal phase correction associated only with the piston motion of the mirrors. A description of the Phase Retrieval problem is first presented. The Systematic Image-Based Optical Alignment (SIBOA) Testbeb is then described. A Discrete Fourier Transform (DFT) is necessary to transfer the incoming wavefront (or estimate of phase error) into the spatial frequency domain to compare it with the image. A method for reducing the DFT to seven scalar/matrix multiplications is presented. A genetic algorithm is then used to search for the phase error. The results of this new algorithm on a test problem are presented.

Structure function monitor

DOEpatents

McGraw, John T [Placitas, NM; Zimmer, Peter C [Albuquerque, NM; Ackermann, Mark R [Albuquerque, NM

2012-01-24

Methods and apparatus for a structure function monitor provide for generation of parameters characterizing a refractive medium. In an embodiment, a structure function monitor acquires images of a pupil plane and an image plane and, from these images, retrieves the phase over an aperture, unwraps the retrieved phase, and analyzes the unwrapped retrieved phase. In an embodiment, analysis yields atmospheric parameters measured at spatial scales from zero to the diameter of a telescope used to collect light from a source.

Encoding and immediate retrieval tasks in patients with epilepsy: A functional MRI study of verbal and visual memory.

PubMed

Saddiki, Najat; Hennion, Sophie; Viard, Romain; Ramdane, Nassima; Lopes, Renaud; Baroncini, Marc; Szurhaj, William; Reyns, Nicolas; Pruvo, Jean Pierre; Delmaire, Christine

2018-05-01

Medial lobe temporal structures and more specifically the hippocampus play a decisive role in episodic memory. Most of the memory functional magnetic resonance imaging (fMRI) studies evaluate the encoding phase; the retrieval phase being performed outside the MRI. We aimed to determine the ability to reveal greater hippocampal fMRI activations during retrieval phase. Thirty-five epileptic patients underwent a two-step memory fMRI. During encoding phase, subjects were requested to identify the feminine or masculine gender of faces and words presented, in order to encourage stimulus encoding. One hour after, during retrieval phase, subjects had to recognize the word and face. We used an event-related design to identify hippocampal activations. There was no significant difference between patients with left temporal lobe epilepsy, patients with right temporal lobe epilepsy and patients with extratemporal lobe epilepsy on verbal and visual learning task. For words, patients demonstrated significantly more bilateral hippocampal activation for retrieval task than encoding task and when the tasks were associated than during encoding alone. Significant difference was seen between face-encoding alone and face retrieval alone. This study demonstrates the essential contribution of the retrieval task during a fMRI memory task but the number of patients with hippocampal activations was greater when the two tasks were taken into account. Copyright © 2018. Published by Elsevier Masson SAS.

Fast non-interferometric iterative phase retrieval for holographic data storage.

PubMed

Lin, Xiao; Huang, Yong; Shimura, Tsutomu; Fujimura, Ryushi; Tanaka, Yoshito; Endo, Masao; Nishimoto, Hajimu; Liu, Jinpeng; Li, Yang; Liu, Ying; Tan, Xiaodi

2017-12-11

Fast non-interferometric phase retrieval is a very important technique for phase-encoded holographic data storage and other phase based applications due to its advantage of easy implementation, simple system setup, and robust noise tolerance. Here we present an iterative non-interferometric phase retrieval for 4-level phase encoded holographic data storage based on an iterative Fourier transform algorithm and known portion of the encoded data, which increases the storage code rate to two-times that of an amplitude based method. Only a single image at the Fourier plane of the beam is captured for the iterative reconstruction. Since beam intensity at the Fourier plane of the reconstructed beam is more concentrated than the reconstructed beam itself, the requirement of diffractive efficiency of the recording media is reduced, which will improve the dynamic range of recording media significantly. The phase retrieval only requires 10 iterations to achieve a less than 5% phase data error rate, which is successfully demonstrated by recording and reconstructing a test image data experimentally. We believe our method will further advance the holographic data storage technique in the era of big data.

Parietal EEG alpha suppression time of memory retrieval reflects memory load while the alpha power of memory maintenance is a composite of the visual process according to simultaneous and successive Sternberg memory tasks.

PubMed

Okuhata, Shiho; Kusanagi, Takuya; Kobayashi, Tetsuo

2013-10-25

The present study investigated EEG alpha activity during visual Sternberg memory tasks using two different stimulus presentation modes to elucidate how the presentation mode affected parietal alpha activity. EEGs were recorded from 10 healthy adults during the Sternberg tasks in which memory items were presented simultaneously and successively. EEG power and suppression time (ST) in the alpha band (8-13Hz) were computed for the memory maintenance and retrieval phases. The alpha activity differed according to the presentation mode during the maintenance phase but not during the retrieval phase. Results indicated that parietal alpha power recorded during the maintenance phase did not reflect the memory load alone. In contrast, ST during the retrieval phase increased with the memory load for both presentation modes, indicating a serial memory scanning process, regardless of the presentation mode. These results indicate that there was a dynamic transition in the memory process from the maintenance phase, which was sensitive to external factors, toward the retrieval phase, during which the process converged on the sequential scanning process, the Sternberg task essentially required. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Phase retrieval by coherent modulation imaging

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Fucai; Chen, Bo; Morrison, Graeme R.

Phase retrieval is a long-standing problem in imaging when only the intensity of the wavefield can be recorded. Coherent diffraction imaging (CDI) is a lensless technique that uses iterative algorithms to recover amplitude and phase contrast images from diffraction intensity data. For general samples, phase retrieval from a single diffraction pattern has been an algorithmic and experimental challenge. Here we report a method of phase retrieval that uses a known modulation of the sample exit-wave. This coherent modulation imaging (CMI) method removes inherent ambiguities of CDI and uses a reliable, rapidly converging iterative algorithm involving three planes. It works formore » extended samples, does not require tight support for convergence, and relaxes dynamic range requirements on the detector. CMI provides a robust method for imaging in materials and biological science, while its single-shot capability will benefit the investigation of dynamical processes with pulsed sources, such as X-ray free electron laser.« less

Phase retrieval by coherent modulation imaging

DOE PAGES

Zhang, Fucai; Chen, Bo; Morrison, Graeme R.; ...

2016-11-18

Phase retrieval is a long-standing problem in imaging when only the intensity of the wavefield can be recorded. Coherent diffraction imaging (CDI) is a lensless technique that uses iterative algorithms to recover amplitude and phase contrast images from diffraction intensity data. For general samples, phase retrieval from a single diffraction pattern has been an algorithmic and experimental challenge. Here we report a method of phase retrieval that uses a known modulation of the sample exit-wave. This coherent modulation imaging (CMI) method removes inherent ambiguities of CDI and uses a reliable, rapidly converging iterative algorithm involving three planes. It works formore » extended samples, does not require tight support for convergence, and relaxes dynamic range requirements on the detector. CMI provides a robust method for imaging in materials and biological science, while its single-shot capability will benefit the investigation of dynamical processes with pulsed sources, such as X-ray free electron laser.« less

Phase retrieval by coherent modulation imaging.

PubMed

Zhang, Fucai; Chen, Bo; Morrison, Graeme R; Vila-Comamala, Joan; Guizar-Sicairos, Manuel; Robinson, Ian K

2016-11-18

Phase retrieval is a long-standing problem in imaging when only the intensity of the wavefield can be recorded. Coherent diffraction imaging is a lensless technique that uses iterative algorithms to recover amplitude and phase contrast images from diffraction intensity data. For general samples, phase retrieval from a single-diffraction pattern has been an algorithmic and experimental challenge. Here we report a method of phase retrieval that uses a known modulation of the sample exit wave. This coherent modulation imaging method removes inherent ambiguities of coherent diffraction imaging and uses a reliable, rapidly converging iterative algorithm involving three planes. It works for extended samples, does not require tight support for convergence and relaxes dynamic range requirements on the detector. Coherent modulation imaging provides a robust method for imaging in materials and biological science, while its single-shot capability will benefit the investigation of dynamical processes with pulsed sources, such as X-ray free-electron lasers.

DOLPHIn—Dictionary Learning for Phase Retrieval

NASA Astrophysics Data System (ADS)

Tillmann, Andreas M.; Eldar, Yonina C.; Mairal, Julien

2016-12-01

We propose a new algorithm to learn a dictionary for reconstructing and sparsely encoding signals from measurements without phase. Specifically, we consider the task of estimating a two-dimensional image from squared-magnitude measurements of a complex-valued linear transformation of the original image. Several recent phase retrieval algorithms exploit underlying sparsity of the unknown signal in order to improve recovery performance. In this work, we consider such a sparse signal prior in the context of phase retrieval, when the sparsifying dictionary is not known in advance. Our algorithm jointly reconstructs the unknown signal - possibly corrupted by noise - and learns a dictionary such that each patch of the estimated image can be sparsely represented. Numerical experiments demonstrate that our approach can obtain significantly better reconstructions for phase retrieval problems with noise than methods that cannot exploit such "hidden" sparsity. Moreover, on the theoretical side, we provide a convergence result for our method.

Phase retrieval using regularization method in intensity correlation imaging

NASA Astrophysics Data System (ADS)

Li, Xiyu; Gao, Xin; Tang, Jia; Lu, Changming; Wang, Jianli; Wang, Bin

2014-11-01

Intensity correlation imaging(ICI) method can obtain high resolution image with ground-based low precision mirrors, in the imaging process, phase retrieval algorithm should be used to reconstituted the object's image. But the algorithm now used(such as hybrid input-output algorithm) is sensitive to noise and easy to stagnate. However the signal-to-noise ratio of intensity interferometry is low especially in imaging astronomical objects. In this paper, we build the mathematical model of phase retrieval and simplified it into a constrained optimization problem of a multi-dimensional function. New error function was designed by noise distribution and prior information using regularization method. The simulation results show that the regularization method can improve the performance of phase retrieval algorithm and get better image especially in low SNR condition

Prefrontal responses to digit span memory phases in patients with post-traumatic stress disorder (PTSD): a functional near infrared spectroscopy study.

PubMed

Tian, Fenghua; Yennu, Amarnath; Smith-Osborne, Alexa; Gonzalez-Lima, F; North, Carol S; Liu, Hanli

2014-01-01

Neuroimaging studies of post-traumatic stress disorder (PTSD)-related memory impairments have consistently implicated abnormal activities in the frontal and parietal lobes. However, most studies have used block designs and could not dissociate the multiple phases of working memory. In this study, the involvement of the prefrontal cortex in working memory phases was assessed among veterans with PTSD and age-/gender-matched healthy controls. Multichannel functional near infrared spectroscopy (fNIRS) was utilized to measure prefrontal cortex hemodynamic activations during memory of neutral (i.e., not trauma-related) forward and backward digit span tasks. An event-related experimental design was utilized to dissociate the different phases (i.e., encoding, maintenance and retrieval) of working memory. The healthy controls showed robust hemodynamic activations during the encoding and retrieval processes. In contrast, the veterans with PTSD were found to have activations during the encoding process, but followed by distinct deactivations during the retrieval process. The PTSD participants, but not the controls, appeared to suppress prefrontal activity during memory retrieval. This deactivation was more pronounced in the right dorsolateral prefrontal cortex during the retrieval phase. These deactivations in PTSD patients might implicate an active inhibition of dorsolateral prefrontal neural activity during retrieval of working memory.

Bilateral Saccadic Eye Movements and Tactile Stimulation, but Not Auditory Stimulation, Enhance Memory Retrieval

ERIC Educational Resources Information Center

Nieuwenhuis, Sander; Elzinga, Bernet M.; Ras, Priscilla H.; Berends, Floris; Duijs, Peter; Samara, Zoe; Slagter, Heleen A.

2013-01-01

Recent research has shown superior memory retrieval when participants make a series of horizontal saccadic eye movements between the memory encoding phase and the retrieval phase compared to participants who do not move their eyes or move their eyes vertically. It has been hypothesized that the rapidly alternating activation of the two hemispheres…

Visualizing and improving the robustness of phase retrieval algorithms

DOE PAGES

Tripathi, Ashish; Leyffer, Sven; Munson, Todd; ...

2015-06-01

Coherent x-ray diffractive imaging is a novel imaging technique that utilizes phase retrieval and nonlinear optimization methods to image matter at nanometer scales. We explore how the convergence properties of a popular phase retrieval algorithm, Fienup's HIO, behave by introducing a reduced dimensionality problem allowing us to visualize and quantify convergence to local minima and the globally optimal solution. We then introduce generalizations of HIO that improve upon the original algorithm's ability to converge to the globally optimal solution.

Visualizing and improving the robustness of phase retrieval algorithms

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tripathi, Ashish; Leyffer, Sven; Munson, Todd

Coherent x-ray diffractive imaging is a novel imaging technique that utilizes phase retrieval and nonlinear optimization methods to image matter at nanometer scales. We explore how the convergence properties of a popular phase retrieval algorithm, Fienup's HIO, behave by introducing a reduced dimensionality problem allowing us to visualize and quantify convergence to local minima and the globally optimal solution. We then introduce generalizations of HIO that improve upon the original algorithm's ability to converge to the globally optimal solution.

Phase retrieval from local measurements in two dimensions

NASA Astrophysics Data System (ADS)

Iwen, Mark; Preskitt, Brian; Saab, Rayan; Viswanathan, Aditya

2017-08-01

The phase retrieval problem has appeared in a multitude of applications for decades. While ad hoc solutions have existed since the early 1970s, recent developments have provided algorithms that offer promising theoretical guarantees under increasingly realistic assumptions. Motivated by ptychographic imaging, we generalize a recent result on phase retrieval of a one dimensional objective vector x ∈ ℂd to recover a two dimensional sample Q ∈ ℂd x d from phaseless measurements, using a tensor product formulation to extend the previous work.

Emotional memory retrieval. rTMS stimulation on left DLPFC increases the positive memories.

PubMed

Balconi, Michela; Ferrari, Chiara

2012-09-01

A suggestive hypothesis proposed that the lateral prefrontal cortex (LPFC) may be identified as the site of emotion-memory integration, since it was shown to be sensitive to the encoding and retrieval of emotional content. In the present research we explored the role of the dorsolateral prefrontal cortex (DLPFC) in memory retrieval of positive vs. negative emotional stimuli. This effect was analyzed by using an rTMS paradigm that induced a cortical activation of the left DLPFC. Subjects were required to perform a task consisting of two experimental phases: an encoding phase, where some lists composed by positive and negative emotional words were presented to the subjects; a retrieval phase, where the old stimuli and the new stimuli were presented for a recognition performance. The rTMS stimulation was provided during the retrieval phase over the left DLPFC. We found that the rTMS stimulation over this area affects the memory retrieval of positive emotional material, with higher memory efficiency (reduced RTs). This result suggested that left DLPFC activation promotes the memory retrieval of emotional information. Secondly, the valence model of emotional cue processing may explain decreasing of RTs, by pointing out the distinct role the left hemisphere has in positive emotional cue processing.

Theory of the amplitude-phase retrieval in any linear-transform system and its applications

NASA Astrophysics Data System (ADS)

Yang, Guozhen; Gu, Ben-Yuan; Dong, Bi-Zhen

1992-12-01

This paper is a summary of the theory of the amplitude-phase retrieval problem in any linear transform system and its applications based on our previous works in the past decade. We describe the general statement on the amplitude-phase retrieval problem in an imaging system and derive a set of equations governing the amplitude-phase distribution in terms of the rigorous mathematical derivation. We then show that, by using these equations and an iterative algorithm, a variety of amplitude-phase problems can be successfully handled. We carry out the systematic investigations and comprehensive numerical calculations to demonstrate the utilization of this new algorithm in various transform systems. For instance, we have achieved the phase retrieval from two intensity measurements in an imaging system with diffraction loss (non-unitary transform), both theoretically and experimentally, and the recovery of model real image from its Hartley-transform modulus only in one and two dimensional cases. We discuss the achievement of the phase retrieval problem from a single intensity only based on the sampling theorem and our algorithm. We also apply this algorithm to provide an optimal design of the phase-adjusted plate for a phase-adjustment focusing laser accelerator and a design approach of single phase-only element for implementing optical interconnect. In order to closely simulate the really measured data, we examine the reconstruction of image from its spectral modulus corrupted by a random noise in detail. The results show that the convergent solution can always be obtained and the quality of the recovered image is satisfactory. We also indicated the relationship and distinction between our algorithm and the original Gerchberg- Saxton algorithm. From these studies, we conclude that our algorithm shows great capability to deal with the comprehensive phase-retrieval problems in the imaging system and the inverse problem in solid state physics. It may open a new way to solve important inverse source problems extensively appearing in physics.

Forced phase-locked states and information retrieval in a two-layer network of oscillatory neurons with directional connectivity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kazantsev, Victor; Pimashkin, Alexey; Department of Neurodynamics and Neurobiology, Nizhny Novgorod State University, 23 Gagarin Ave., 603950 Nizhny Novgorod

We propose two-layer architecture of associative memory oscillatory network with directional interlayer connectivity. The network is capable to store information in the form of phase-locked (in-phase and antiphase) oscillatory patterns. The first (input) layer takes an input pattern to be recognized and their units are unidirectionally connected with all units of the second (control) layer. The connection strengths are weighted using the Hebbian rule. The output (retrieved) patterns appear as forced-phase locked states of the control layer. The conditions are found and analytically expressed for pattern retrieval in response on incoming stimulus. It is shown that the system is capablemore » to recover patterns with a certain level of distortions or noises in their profiles. The architecture is implemented with the Kuramoto phase model and using synaptically coupled neural oscillators with spikes. It is found that the spiking model is capable to retrieve patterns using the spiking phase that translates memorized patterns into the spiking phase shifts at different time scales.« less

Negative emotion enhances mnemonic precision and subjective feelings of remembering in visual long-term memory.

PubMed

Xie, Weizhen; Zhang, Weiwei

2017-09-01

Negative emotion sometimes enhances memory (higher accuracy and/or vividness, e.g., flashbulb memories). The present study investigates whether it is the qualitative (precision) or quantitative (the probability of successful retrieval) aspect of memory that drives these effects. In a visual long-term memory task, observers memorized colors (Experiment 1a) or orientations (Experiment 1b) of sequentially presented everyday objects under negative, neutral, or positive emotions induced with International Affective Picture System images. In a subsequent test phase, observers reconstructed objects' colors or orientations using the method of adjustment. We found that mnemonic precision was enhanced under the negative condition relative to the neutral and positive conditions. In contrast, the probability of successful retrieval was comparable across the emotion conditions. Furthermore, the boost in memory precision was associated with elevated subjective feelings of remembering (vividness and confidence) and metacognitive sensitivity in Experiment 2. Altogether, these findings suggest a novel precision-based account for emotional memories. Copyright © 2017 Elsevier B.V. All rights reserved.

Phase diagram of restricted Boltzmann machines and generalized Hopfield networks with arbitrary priors.

PubMed

Barra, Adriano; Genovese, Giuseppe; Sollich, Peter; Tantari, Daniele

2018-02-01

Restricted Boltzmann machines are described by the Gibbs measure of a bipartite spin glass, which in turn can be seen as a generalized Hopfield network. This equivalence allows us to characterize the state of these systems in terms of their retrieval capabilities, both at low and high load, of pure states. We study the paramagnetic-spin glass and the spin glass-retrieval phase transitions, as the pattern (i.e., weight) distribution and spin (i.e., unit) priors vary smoothly from Gaussian real variables to Boolean discrete variables. Our analysis shows that the presence of a retrieval phase is robust and not peculiar to the standard Hopfield model with Boolean patterns. The retrieval region becomes larger when the pattern entries and retrieval units get more peaked and, conversely, when the hidden units acquire a broader prior and therefore have a stronger response to high fields. Moreover, at low load retrieval always exists below some critical temperature, for every pattern distribution ranging from the Boolean to the Gaussian case.

Uncertainties in Cloud Phase and Optical Thickness Retrievals from the Earth Polychromatic Imaging Camera (EPIC)

NASA Technical Reports Server (NTRS)

Meyer, Kerry; Yang, Yuekui; Platnick, Steven

2016-01-01

This paper presents an investigation of the expected uncertainties of a single channel cloud optical thickness (COT) retrieval technique, as well as a simple cloud-temperature-threshold-based thermodynamic phase approach, in support of the Deep Space Climate Observatory (DSCOVR) mission. DSCOVR cloud products will be derived from Earth Polychromatic Imaging Camera (EPIC) observations in the ultraviolet and visible spectra. Since EPIC is not equipped with a spectral channel in the shortwave or mid-wave infrared that is sensitive to cloud effective radius (CER), COT will be inferred from a single visible channel with the assumption of appropriate CER values for liquid and ice phase clouds. One month of Aqua MODIS daytime granules from April 2005 is selected for investigating cloud phase sensitivity, and a subset of these granules that has similar EPIC sun-view geometry is selected for investigating COT uncertainties. EPIC COT retrievals are simulated with the same algorithm as the operational MODIS cloud products (MOD06), except using fixed phase-dependent CER values. Uncertainty estimates are derived by comparing the single channel COT retrievals with the baseline bi-spectral MODIS retrievals. Results show that a single channel COT retrieval is feasible for EPIC. For ice clouds, single channel retrieval errors are minimal (less than 2 percent) due to the particle- size insensitivity of the assumed ice crystal (i.e., severely roughened aggregate of hexagonal columns) scattering properties at visible wavelengths, while for liquid clouds the error is mostly limited to within 10 percent, although for thin clouds (COT less than 2) the error can be higher. Potential uncertainties in EPIC cloud masking and cloud temperature retrievals are not considered in this study.

Uncertainties in cloud phase and optical thickness retrievals from the Earth Polychromatic Imaging Camera (EPIC)

PubMed Central

Meyer, Kerry; Yang, Yuekui; Platnick, Steven

2018-01-01

This paper presents an investigation of the expected uncertainties of a single channel cloud optical thickness (COT) retrieval technique, as well as a simple cloud temperature threshold based thermodynamic phase approach, in support of the Deep Space Climate Observatory (DSCOVR) mission. DSCOVR cloud products will be derived from Earth Polychromatic Imaging Camera (EPIC) observations in the ultraviolet and visible spectra. Since EPIC is not equipped with a spectral channel in the shortwave or mid-wave infrared that is sensitive to cloud effective radius (CER), COT will be inferred from a single visible channel with the assumption of appropriate CER values for liquid and ice phase clouds. One month of Aqua MODIS daytime granules from April 2005 is selected for investigating cloud phase sensitivity, and a subset of these granules that has similar EPIC sun-view geometry is selected for investigating COT uncertainties. EPIC COT retrievals are simulated with the same algorithm as the operational MODIS cloud products (MOD06), except using fixed phase-dependent CER values. Uncertainty estimates are derived by comparing the single channel COT retrievals with the baseline bi-spectral MODIS retrievals. Results show that a single channel COT retrieval is feasible for EPIC. For ice clouds, single channel retrieval errors are minimal (< 2%) due to the particle size insensitivity of the assumed ice crystal (i.e., severely roughened aggregate of hexagonal columns) scattering properties at visible wavelengths, while for liquid clouds the error is mostly limited to within 10%, although for thin clouds (COT < 2) the error can be higher. Potential uncertainties in EPIC cloud masking and cloud temperature retrievals are not considered in this study. PMID:29619116

Uncertainties in cloud phase and optical thickness retrievals from the Earth Polychromatic Imaging Camera (EPIC).

PubMed

Meyer, Kerry; Yang, Yuekui; Platnick, Steven

2016-01-01

This paper presents an investigation of the expected uncertainties of a single channel cloud optical thickness (COT) retrieval technique, as well as a simple cloud temperature threshold based thermodynamic phase approach, in support of the Deep Space Climate Observatory (DSCOVR) mission. DSCOVR cloud products will be derived from Earth Polychromatic Imaging Camera (EPIC) observations in the ultraviolet and visible spectra. Since EPIC is not equipped with a spectral channel in the shortwave or mid-wave infrared that is sensitive to cloud effective radius (CER), COT will be inferred from a single visible channel with the assumption of appropriate CER values for liquid and ice phase clouds. One month of Aqua MODIS daytime granules from April 2005 is selected for investigating cloud phase sensitivity, and a subset of these granules that has similar EPIC sun-view geometry is selected for investigating COT uncertainties. EPIC COT retrievals are simulated with the same algorithm as the operational MODIS cloud products (MOD06), except using fixed phase-dependent CER values. Uncertainty estimates are derived by comparing the single channel COT retrievals with the baseline bi-spectral MODIS retrievals. Results show that a single channel COT retrieval is feasible for EPIC. For ice clouds, single channel retrieval errors are minimal (< 2%) due to the particle size insensitivity of the assumed ice crystal (i.e., severely roughened aggregate of hexagonal columns) scattering properties at visible wavelengths, while for liquid clouds the error is mostly limited to within 10%, although for thin clouds (COT < 2) the error can be higher. Potential uncertainties in EPIC cloud masking and cloud temperature retrievals are not considered in this study.

Uncertainties in cloud phase and optical thickness retrievals from the Earth Polychromatic Imaging Camera (EPIC)

NASA Astrophysics Data System (ADS)

Meyer, Kerry; Yang, Yuekui; Platnick, Steven

2016-04-01

This paper presents an investigation of the expected uncertainties of a single-channel cloud optical thickness (COT) retrieval technique, as well as a simple cloud-temperature-threshold-based thermodynamic phase approach, in support of the Deep Space Climate Observatory (DSCOVR) mission. DSCOVR cloud products will be derived from Earth Polychromatic Imaging Camera (EPIC) observations in the ultraviolet and visible spectra. Since EPIC is not equipped with a spectral channel in the shortwave or mid-wave infrared that is sensitive to cloud effective radius (CER), COT will be inferred from a single visible channel with the assumption of appropriate CER values for liquid and ice phase clouds. One month of Aqua MODerate-resolution Imaging Spectroradiometer (MODIS) daytime granules from April 2005 is selected for investigating cloud phase sensitivity, and a subset of these granules that has similar EPIC Sun-view geometry is selected for investigating COT uncertainties. EPIC COT retrievals are simulated with the same algorithm as the operational MODIS cloud products (MOD06), except using fixed phase-dependent CER values. Uncertainty estimates are derived by comparing the single-channel COT retrievals with the baseline bi-spectral MODIS retrievals. Results show that a single-channel COT retrieval is feasible for EPIC. For ice clouds, single-channel retrieval errors are minimal (< 2 %) due to the particle size insensitivity of the assumed ice crystal (i.e., severely roughened aggregate of hexagonal columns) scattering properties at visible wavelengths, while for liquid clouds the error is mostly limited to within 10 %, although for thin clouds (COT < 2) the error can be higher. Potential uncertainties in EPIC cloud masking and cloud temperature retrievals are not considered in this study.

Interfering effects of retrieval in learning new information.

PubMed

Finn, Bridgid; Roediger, Henry L

2013-11-01

In 7 experiments, we explored the role of retrieval in associative updating, that is, in incorporating new information into an associative memory. We tested the hypothesis that retrieval would facilitate incorporating a new contextual detail into a learned association. Participants learned 3 pieces of information-a person's face, name, and profession (in Experiments 1-5). In the 1st phase, participants in all conditions learned faces and names. In the 2nd phase, participants either restudied the face-name pair (the restudy condition) or were given the face and asked to retrieve the name (the test condition). In the 3rd phase, professions were presented for study just after restudy or testing. Our prediction was that the new information (the profession) would be more readily learned following retrieval of the face-name association compared to restudy of the face-name association. However, we found that the act of retrieval generally undermined acquisition of new associations rather than facilitating them. This detrimental effect emerged on both immediate and delayed tests. Further, the effect was not due to selective attention to feedback because we found impairment whether or not feedback was provided after the Phase 2 test. The data are novel in showing that the act of retrieving information can inhibit the ability to learn new information shortly thereafter. The results are difficult to accommodate within current theories that mostly emphasize benefits of retrieval for learning. PsycINFO Database Record (c) 2013 APA, all rights reserved.

Phase Retrieval for Radio Telescope and Antenna Control

NASA Technical Reports Server (NTRS)

Dean, Bruce

2011-01-01

Phase-retrieval is a general term used in optics to describe the estimation of optical imperfections or "aberrations." The purpose of this innovation is to develop the application of phase retrieval to radio telescope and antenna control in the millimeter wave band. Earlier techniques do not approximate the incoherent subtraction process as a coherent propagation. This approximation reduces the noise in the data and allows a straightforward application of conventional phase retrieval techniques for radio telescope and antenna control. The application of iterative-transform phase retrieval to radio telescope and antenna control is made by approximating the incoherent subtraction process as a coherent propagation. Thus, for systems utilizing both positive and negative polarity feeds, this approximation allows both surface and alignment errors to be assessed without the use of additional hardware or laser metrology. Knowledge of the antenna surface profile allows errors to be corrected at a given surface temperature and observing angle. In addition to imperfections of the antenna surface figure, the misalignment of multiple antennas operating in unison can reduce or degrade the signal-to-noise ratio of the received or broadcast signals. This technique also has application to the alignment of antenna array configurations.

Phase retrieval on broadband and under-sampled images for the JWST testbed telescope

NASA Astrophysics Data System (ADS)

Smith, J. Scott; Aronstein, David L.; Dean, Bruce H.; Acton, D. Scott

2009-08-01

The James Webb Space Telescope (JWST) consists of an optical telescope element (OTE) that sends light to five science instruments. The initial steps for commissioning the telescope are performed with the Near-Infrared Camera (NIRCam) instrument, but low-order optical aberrations in the remaining science instruments must be determined (using phase retrieval) in order to ensure good performance across the entire field of view. These remaining instruments were designed to collect science data, and not to serve as wavefront sensors. Thus, the science cameras are not ideal phase-retrieval imagers for several reasons: they record under-sampled data and have a limited range of diversity defocus, and only one instrument has an internal, narrowband filter. To address these issues, we developed the capability of sensing these aberrations using an extension of image-based iterative-transform phase retrieval called Variable Sampling Mapping (VSM). The results show that VSM-based phase retrieval is capable of sensing low-order aberrations to a few nm RMS from images that are consistent with the non-ideal conditions expected during JWST multi-field commissioning. The algorithm is validated using data collected from the JWST Testbed Telescope (TBT).

Retrieving cirrus microphysical properties from stellar aureoles

NASA Astrophysics Data System (ADS)

DeVore, J. G.; Kristl, J. A.; Rappaport, S. A.

2013-06-01

The aureoles around stars caused by thin cirrus limit nighttime measurement opportunities for ground-based astronomy, but can provide information on high-altitude ice crystals for climate research. In this paper we attempt to demonstrate quantitatively how this works. Aureole profiles can be followed out to ~0.2° from stars and ~0.5° from Jupiter. Interpretation of diffracted starlight is similar to that for sunlight, but emphasizes larger particles. Stellar diffraction profiles are very distinctive, typically being approximately flat out to a critical angle followed by gradually steepening power-law falloff with slope less steep than -3. Using the relationship between the phase function for diffraction and the average Fourier transform of the projected area of complex ice crystals, we show that defining particle size in terms of average projected area normal to the propagation direction of the starlight leads to a simple, analytic approximation representing large-particle diffraction that is nearly independent of crystal habit. A similar analytic approximation for the diffraction aureole allows it to be separated from the point spread function and the sky background. Multiple scattering is deconvolved using the Hankel transform leading to the diffraction phase function. Application of constrained numerical inversion to the phase function then yields a solution for the particle size distribution in the range between ~50 μm and ~400 μm. Stellar aureole measurements can provide one of the very few, as well as least expensive, methods for retrieving cirrus microphysical properties from ground-based observations.

How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm should look like?: A Comment on two papers by Steelman et al. and Schürmann et al. Read the Responses to this Comment: e201800091 and e201800095.

PubMed

Yurkin, Maxim A

2018-05-02

In recent papers Steelman et al. ("Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies") and Schürmann et al. ("Cell nuclei have lower refractive index and mass density than cytoplasm") obtained quantitative phase images of whole cells of various types and corresponding isolated nuclei and concluded that the refractive index (RI) of the nucleus is significantly smaller than that of the cytoplasm. The comment shows that this conclusion and assumptions used in retrieving the RI necessarily imply a characteristic dip in the center of the whole-cell phase images. This dip is not present in any of the phase images in the discussed papers, which is a strong argument against the conclusion of smaller nucleus RI. It is also discussed whether a different processing of the phase images can help to clarify this issue. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Optically secured information retrieval using two authenticated phase-only masks.

PubMed

Wang, Xiaogang; Chen, Wen; Mei, Shengtao; Chen, Xudong

2015-10-23

We propose an algorithm for jointly designing two phase-only masks (POMs) that allow for the encryption and noise-free retrieval of triple images. The images required for optical retrieval are first stored in quick-response (QR) codes for noise-free retrieval and flexible readout. Two sparse POMs are respectively calculated from two different images used as references for authentication based on modified Gerchberg-Saxton algorithm (GSA) and pixel extraction, and are then used as support constraints in a modified double-phase retrieval algorithm (MPRA), together with the above-mentioned QR codes. No visible information about the target images or the reference images can be obtained from each of these authenticated POMs. This approach allows users to authenticate the two POMs used for image reconstruction without visual observation of the reference images. It also allows user to friendly access and readout with mobile devices.

Optically secured information retrieval using two authenticated phase-only masks

PubMed Central

Wang, Xiaogang; Chen, Wen; Mei, Shengtao; Chen, Xudong

2015-01-01

We propose an algorithm for jointly designing two phase-only masks (POMs) that allow for the encryption and noise-free retrieval of triple images. The images required for optical retrieval are first stored in quick-response (QR) codes for noise-free retrieval and flexible readout. Two sparse POMs are respectively calculated from two different images used as references for authentication based on modified Gerchberg-Saxton algorithm (GSA) and pixel extraction, and are then used as support constraints in a modified double-phase retrieval algorithm (MPRA), together with the above-mentioned QR codes. No visible information about the target images or the reference images can be obtained from each of these authenticated POMs. This approach allows users to authenticate the two POMs used for image reconstruction without visual observation of the reference images. It also allows user to friendly access and readout with mobile devices. PMID:26494213

Validation of MODIS Dust Aerosol Retrieval and Development Ambient Dust Phase Function using PRIDE Data

NASA Technical Reports Server (NTRS)

Remer, Lorraine A.; Lau, William (Technical Monitor)

2002-01-01

The PRIDE data set of MODIS aerosol retrievals co-located with sunphotometer measurements provides the basis of MODIS validation in a dust environment. The sunphotometer measurements include AERONET automatic instruments, land-based Microtops instruments, ship-board Microtops instruments and the AATS-6 aboard the Navajo aircraft. Analysis of these data indicate that the MODIS retrieval is within pre-launch estimates of uncertainty within the spectral range of 600-900 nm. However, the MODIS algorithm consistently retrieves smaller particles than reality thus leading to incorrect spectral response outside of the 600-900 nm range and improper size information. Further analysis of MODIS retrievals in other dust environments shows the inconsistencies are due to nonspherical effects in the phase function. These data are used to develop an ambient phase function for dust aerosol to be used for remote sensing purposes.

Optically secured information retrieval using two authenticated phase-only masks

NASA Astrophysics Data System (ADS)

Wang, Xiaogang; Chen, Wen; Mei, Shengtao; Chen, Xudong

2015-10-01

We propose an algorithm for jointly designing two phase-only masks (POMs) that allow for the encryption and noise-free retrieval of triple images. The images required for optical retrieval are first stored in quick-response (QR) codes for noise-free retrieval and flexible readout. Two sparse POMs are respectively calculated from two different images used as references for authentication based on modified Gerchberg-Saxton algorithm (GSA) and pixel extraction, and are then used as support constraints in a modified double-phase retrieval algorithm (MPRA), together with the above-mentioned QR codes. No visible information about the target images or the reference images can be obtained from each of these authenticated POMs. This approach allows users to authenticate the two POMs used for image reconstruction without visual observation of the reference images. It also allows user to friendly access and readout with mobile devices.

Understanding Differences Between Co-Incident CloudSat, Aqua/MODIS and NOAA18 MHS Ice water Path Retrievals Over the Tropical Oceans

NASA Technical Reports Server (NTRS)

Pittman, Jasna; Robertson, Franklin; Blankenship, Clay

2008-01-01

Accurate measurement of the physical and radiative properties of clouds and their representation in climate models continues to be a challe nge. Model parameterizations are still subject to a large number of t unable parameters; furthermore, accurate and representative in situ o bservations are very sparse, and satellite observations historically have significant quantitative uncertainties, particularly with respect to particle size distribution (PSD) and cloud phase. Ice Water Path (IWP), or amount of ice present in a cloud column, is an important cl oud property to accurately quantify, because it is an integral measur e of the microphysical properties of clouds and the cloud feedback pr ocesses in the climate system. This paper investigates near co-incident retrievals of IWP over tropical oceans using three diverse measurem ent systems: radar from CloudSat, Vis/IR from Aqua/MODIS, and microwa ve from NOAA-18IMHS. CloudSat 94 GHz radar measurements provide high resolution vertical and along-orbit structure of cloud reflectivity a nd enable IWP (and IWC) retrievals. Overlapping MODIS measurements of cloud optical thickness and phase allow estimates of IWP when cloud tops are identified as being ice. Periodically, NOAA18 becomes co-inci dent in space I time to enable comparison of A-Train measurements to IWP inferred from the 157 and 89 GHz channel radiances. This latter m easurement is effective only for thick convective anvil systems. We s tratify these co-incident data (less than 4 minutes separation) into cirrus only, cirrus overlying liquid water clouds, and precipitating d eep convective clouds. Substantial biases in IWP and ice effective ra dius are found. Systematic differences in these retrievals are consid ered in light of the uncertainties in a priori assumptions ofPSDs, sp ectral sensitivity and algorithm strategies, which have a direct impact on the IWP product.

Characterizing the information content of cloud thermodynamic phase retrievals from the notional PACE OCI shortwave reflectance measurements

NASA Astrophysics Data System (ADS)

Coddington, O. M.; Vukicevic, T.; Schmidt, K. S.; Platnick, S.

2017-08-01

We rigorously quantify the probability of liquid or ice thermodynamic phase using only shortwave spectral channels specific to the National Aeronautics and Space Administration's Moderate Resolution Imaging Spectroradiometer, Visible Infrared Imaging Radiometer Suite, and the notional future Plankton, Aerosol, Cloud, ocean Ecosystem imager. The results show that two shortwave-infrared channels (2135 and 2250 nm) provide more information on cloud thermodynamic phase than either channel alone; in one case, the probability of ice phase retrieval increases from 65 to 82% by combining 2135 and 2250 nm channels. The analysis is performed with a nonlinear statistical estimation approach, the GEneralized Nonlinear Retrieval Analysis (GENRA). The GENRA technique has previously been used to quantify the retrieval of cloud optical properties from passive shortwave observations, for an assumed thermodynamic phase. Here we present the methodology needed to extend the utility of GENRA to a binary thermodynamic phase space (i.e., liquid or ice). We apply formal information content metrics to quantify our results; two of these (mutual and conditional information) have not previously been used in the field of cloud studies.

Evidence for encoding versus retrieval scheduling in the hippocampus by theta phase and acetylcholine

PubMed Central

Douchamps, Vincent; Jeewajee, Ali; Blundell, Pam; Burgess, Neil; Lever, Colin

2013-01-01

The formation of new memories requires new information to be encoded in the face of proactive interference from the past. Two solutions have been proposed for hippocampal region CA1: 1) acetylcholine, released in novelty, selectively suppresses excitatory projections to CA1 from CA3 (mediating the products of retrieval), while sparing entorhinal inputs (mediating novel sensory information); 2) encoding preferentially occurs at the pyramidal-layer theta peak, coincident with input from entorhinal cortex, and retrieval occurs at the trough, coincident with input from CA3, consistent with theta-phase-dependent synaptic plasticity. We examined three predictions of these models: 1) In novel environments, the preferred theta phase of CA1 place cell firing should shift closer to the CA1 pyramidal-layer theta peak, shifting the encoding-retrieval balance towards encoding; 2) The encoding-related shift in novel environments should be disrupted by cholinergic antagonism; 3) In familiar environments, cholinergic antagonism should shift the preferred theta firing phase closer to the theta trough, shifting the encoding-retrieval balance even further towards retrieval. We tested these predictions by recording from CA1 pyramidal cells in freely moving rats as they foraged in open field environments under the influence of scopolamine (an amnestic cholinergic antagonist) or vehicle (saline). Results confirmed all three predictions, supporting both the theta phase and cholinergic models of encoding-vs-retrieval dynamics. Also consistent with cholinergic enhancement of encoding, scopolamine attenuated the formation of distinct spatial representations in a new environment, reducing the extent of place cell “remapping”. PMID:23678113

Retrieval of the atomic displacements in the crystal from the coherent X-ray diffraction pattern.

PubMed

Minkevich, A A; Köhl, M; Escoubas, S; Thomas, O; Baumbach, T

2014-07-01

The retrieval of spatially resolved atomic displacements is investigated via the phases of the direct(real)-space image reconstructed from the strained crystal's coherent X-ray diffraction pattern. It is demonstrated that limiting the spatial variation of the first- and second-order spatial displacement derivatives improves convergence of the iterative phase-retrieval algorithm for displacements reconstructions to the true solution. This approach is exploited to retrieve the displacement in a periodic array of silicon lines isolated by silicon dioxide filled trenches.

X-Ray Phase Imaging for Breast Cancer Detection

DTIC Science & Technology

2012-09-01

the Gerchberg-Saxton algorithm in the Fresnel diffraction regime, and is much more robust against image noise than the TIE-based method. For details...developed efficient coding with the software modules for the image registration, flat-filed correction , and phase retrievals. In addition, we...X, Liu H. 2010. Performance analysis of the attenuation-partition based iterative phase retrieval algorithm for in-line phase-contrast imaging

Resolution enhancement by extrapolation of coherent diffraction images: a quantitative study on the limits and a numerical study of nonbinary and phase objects.

PubMed

Latychevskaia, T; Chushkin, Y; Fink, H-W

2016-10-01

In coherent diffractive imaging, the resolution of the reconstructed object is limited by the numerical aperture of the experimental setup. We present here a theoretical and numerical study for achieving super-resolution by postextrapolation of coherent diffraction images, such as diffraction patterns or holograms. We demonstrate that a diffraction pattern can unambiguously be extrapolated from only a fraction of the entire pattern and that the ratio of the extrapolated signal to the originally available signal is linearly proportional to the oversampling ratio. Although there could be in principle other methods to achieve extrapolation, we devote our discussion to employing iterative phase retrieval methods and demonstrate their limits. We present two numerical studies; namely, the extrapolation of diffraction patterns of nonbinary and that of phase objects together with a discussion of the optimal extrapolation procedure. © 2016 The Authors Journal of Microscopy © 2016 Royal Microscopical Society.

Quantized phase coding and connected region labeling for absolute phase retrieval.

PubMed

Chen, Xiangcheng; Wang, Yuwei; Wang, Yajun; Ma, Mengchao; Zeng, Chunnian

2016-12-12

This paper proposes an absolute phase retrieval method for complex object measurement based on quantized phase-coding and connected region labeling. A specific code sequence is embedded into quantized phase of three coded fringes. Connected regions of different codes are labeled and assigned with 3-digit-codes combining the current period and its neighbors. Wrapped phase, more than 36 periods, can be restored with reference to the code sequence. Experimental results verify the capability of the proposed method to measure multiple isolated objects.

Spaced-antenna wind estimation using an X-band active phased-array weather radar

NASA Astrophysics Data System (ADS)

Venkatesh, Vijay

Over the past few decades, several single radar methods have been developed to probe the kinematic structure of storms. All these methods trade angular-resolution to retrieve the wind-field. To date, the spaced-antenna method has been employed for profiling the ionosphere and the precipitation free lower atmosphere. This work focuses on applying the spaced-antenna method on an X-band active phased-array radar for high resolution horizontal wind-field retrieval from precipitation echoes. The ability to segment the array face into multiple displaced apertures allows for flexible spaced-antenna implementations. The methodology employed herein comprises of Monte-Carlo simulations to optimize the spaced-antenna system design and analysis of real data collected with the designed phased-array system. The contribution that underpins this dissertation is the demonstration of qualitative agreement between spaced-antenna and Doppler beam swinging retrievals based on real data. First, simulations of backscattered electric fields at the antenna array elements are validated using theoretical expressions. Based on the simulations, the degrees of freedom in the spaced-antenna system design are optimized for retrieval of mean baseline wind. We show that the designed X-band spaced-antenna system has lower retrieval uncertainty than the existing S-band spaced-antenna implementation on the NWRT. This is because of the flexibility to synthesize small overlapping apertures and the ability to obtain statistically independent samples at a faster rate at X-band. We then demonstrate a technique to make relative phase-center displacement measurements based on simulations and real data from the phased-array spaced-antenna system. This simple method uses statistics of precipitation echoes and apriori beamwidth measurements to make field repeatable phase-center displacement measurements. Finally, we test the hypothesis that wind-field curvature effects are common to both the spaced-antenna and Doppler beam swinging methods. Based on a close-range winter storm data set, we find that the spaced-antenna and fine-resolution Doppler beam swinging retrievals are in qualitative agreement. The correlation between the spaced-antenna and fine-resolution Doppler beam swinging retrievals was 0.57. The lowered correlation coefficient was, in part, due to the high standard deviation of the DBS retrievals. At high wind-speeds, the spaced-antenna retrievals significantly departed from variational retrievals of mean baseline wind.

Where Is ELSA? The Early to Late Shift in Aging

PubMed Central

Buchler, Norbou; Dobbins, Ian G.; Cabeza, Roberto

2012-01-01

Studies of cognitive and neural aging have recently provided evidence of a shift from an early- to late-onset cognitive control strategy, linked with temporally extended activity in the prefrontal cortex (PFC). It has been uncertain, however, whether this age-related shift is unique to PFC and executive control tasks or whether the functional location might vary depending on the particular cognitive processes that are altered. The present study tested whether an early-to-late shift in aging (ELSA) might emerge in the medial temporal lobes (MTL) during a protracted context memory task comprising both anticipatory cue (retrieval preparation) and retrieval probe (retrieval completion) phases. First, we found reduced MTL activity in older adults during the early retrieval preparation phase coupled with increased MTL activity during the late retrieval completion phase. Second, we found that functional connectivity between MTL and PFC regions was higher during retrieval preparation in young adults but higher during retrieval completion in older adults, suggesting an important interactive relationship between the ELSA pattern in MTL and PFC. Taken together, these results critically suggest that aging results in temporally lagged activity even in regions not typically associated with cognitive control, such as the MTL. PMID:22114083

Label-free nanoscale characterization of red blood cell structure and dynamics using single-shot transport of intensity equation

NASA Astrophysics Data System (ADS)

Poola, Praveen Kumar; John, Renu

2017-10-01

We report the results of characterization of red blood cell (RBC) structure and its dynamics with nanometric sensitivity using transport of intensity equation microscopy (TIEM). Conventional transport of intensity technique requires three intensity images and hence is not suitable for studying real-time dynamics of live biological samples. However, assuming the sample to be homogeneous, phase retrieval using transport of intensity equation has been demonstrated with single defocused measurement with x-rays. We adopt this technique for quantitative phase light microscopy of homogenous cells like RBCs. The main merits of this technique are its simplicity, cost-effectiveness, and ease of implementation on a conventional microscope. The phase information can be easily merged with regular bright-field and fluorescence images to provide multidimensional (three-dimensional spatial and temporal) information without any extra complexity in the setup. The phase measurement from the TIEM has been characterized using polymeric microbeads and the noise stability of the system has been analyzed. We explore the structure and real-time dynamics of RBCs and the subdomain membrane fluctuations using this technique.

Comparison of different numerical treatments for x-ray phase tomography of soft tissue from differential phase projections

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pelliccia, Daniele; Vaz, Raquel; Svalbe, Imants

X-ray imaging of soft tissue is made difficult by their low absorbance. The use of x-ray phase imaging and tomography can significantly enhance the detection of these tissues and several approaches have been proposed to this end. Methods such as analyzer-based imaging or grating interferometry produce differential phase projections that can be used to reconstruct the 3D distribution of the sample refractive index. We report on the quantitative comparison of three different methods to obtain x-ray phase tomography with filtered back-projection from differential phase projections in the presence of noise. The three procedures represent different numerical approaches to solve themore » same mathematical problem, namely phase retrieval and filtered back-projection. It is found that obtaining individual phase projections and subsequently applying a conventional filtered back-projection algorithm produces the best results for noisy experimental data, when compared with other procedures based on the Hilbert transform. The algorithms are tested on simulated phantom data with added noise and the predictions are confirmed by experimental data acquired using a grating interferometer. The experiment is performed on unstained adult zebrafish, an important model organism for biomedical studies. The method optimization described here allows resolution of weak soft tissue features, such as muscle fibers.« less

Phase-retrieval attack free cryptosystem based on cylindrical asymmetric diffraction and double-random phase encoding

NASA Astrophysics Data System (ADS)

Wang, Jun; Li, Xiaowei; Hu, Yuhen; Wang, Qiong-Hua

2018-03-01

A phase-retrieval attack free cryptosystem based on the cylindrical asymmetric diffraction and double-random phase encoding (DRPE) is proposed. The plaintext is abstract as a cylinder, while the observed diffraction and holographic surfaces are concentric cylinders. Therefore, the plaintext can be encrypted through a two-step asymmetric diffraction process with double pseudo random phase masks located on the object surface and the first diffraction surface. After inverse diffraction from a holographic surface to an object surface, the plaintext can be reconstructed using a decryption process. Since the diffraction propagated from the inner cylinder to the outer cylinder is different from that of the reversed direction, the proposed cryptosystem is asymmetric and hence is free of phase-retrieval attack. Numerical simulation results demonstrate the flexibility and effectiveness of the proposed cryptosystem.

Event-related fMRI studies of false memory: An Activation Likelihood Estimation meta-analysis.

PubMed

Kurkela, Kyle A; Dennis, Nancy A

2016-01-29

Over the last two decades, a wealth of research in the domain of episodic memory has focused on understanding the neural correlates mediating false memories, or memories for events that never happened. While several recent qualitative reviews have attempted to synthesize this literature, methodological differences amongst the empirical studies and a focus on only a sub-set of the findings has limited broader conclusions regarding the neural mechanisms underlying false memories. The current study performed a voxel-wise quantitative meta-analysis using activation likelihood estimation to investigate commonalities within the functional magnetic resonance imaging (fMRI) literature studying false memory. The results were broken down by memory phase (encoding, retrieval), as well as sub-analyses looking at differences in baseline (hit, correct rejection), memoranda (verbal, semantic), and experimental paradigm (e.g., semantic relatedness and perceptual relatedness) within retrieval. Concordance maps identified significant overlap across studies for each analysis. Several regions were identified in the general false retrieval analysis as well as multiple sub-analyses, indicating their ubiquitous, yet critical role in false retrieval (medial superior frontal gyrus, left precentral gyrus, left inferior parietal cortex). Additionally, several regions showed baseline- and paradigm-specific effects (hit/perceptual relatedness: inferior and middle occipital gyrus; CRs: bilateral inferior parietal cortex, precuneus, left caudate). With respect to encoding, analyses showed common activity in the left middle temporal gyrus and anterior cingulate cortex. No analysis identified a common cluster of activation in the medial temporal lobe. Copyright © 2015 Elsevier Ltd. All rights reserved.

Determination of wavefront structure for a Hartmann wavefront sensor using a phase-retrieval method.

PubMed

Polo, A; Kutchoukov, V; Bociort, F; Pereira, S F; Urbach, H P

2012-03-26

We apply a phase retrieval algorithm to the intensity pattern of a Hartmann wavefront sensor to measure with enhanced accuracy the phase structure of a Hartmann hole array. It is shown that the rms wavefront error achieved by phase reconstruction is one order of magnitude smaller than the one obtained from a typical centroid algorithm. Experimental results are consistent with a phase measurement performed independently using a Shack-Hartmann wavefront sensor.

Effects of Emotion and Emotional Valence on the Neural Correlates of Episodic Memory Search and Elaboration

PubMed Central

Ford, Jaclyn H.; Morris, John A.; Kensinger, Elizabeth A.

2015-01-01

Successful retrieval of an event includes an initial search phase in which the information is accessed and a subsequent elaboration phase in which an individual expands on event details. Traditionally, functional neuroimaging studies examining episodic memory retrieval either have not made a distinction between these two phases or have focused on the initial search process. The current study used an extended retrieval trial to compare the neural correlates of search and elaboration and to examine the effects of emotion on each phase. Prior to scanning, participants encoded positive, negative, and neutral images paired with neutral titles. After a thirty-minute delay, participants engaged in a scanned recognition task in which they viewed the neutral titles and indicated whether the title had been presented with an image during the study phase. Retrieval was divided into an initial memory search and a subsequent five-second elaboration phase. The current study identified neural differences between the search and elaboration phases, with search being associated with widespread bilateral activations across the entire cortex and elaboration primarily being associated with increased activity in the medial prefrontal cortex. The emotionality of the retrieval target was more influential during search relative to elaboration. However, valence influenced when the effect of emotion was greatest, with search engaging many more regions for positive events than negative ones, but elaboration engaging the dorsomedial prefrontal cortex more for negative events than positive events. PMID:24283491

Hybrid diversity method utilizing adaptive diversity function for recovering unknown aberrations in an optical system

NASA Technical Reports Server (NTRS)

Dean, Bruce H. (Inventor)

2009-01-01

A method of recovering unknown aberrations in an optical system includes collecting intensity data produced by the optical system, generating an initial estimate of a phase of the optical system, iteratively performing a phase retrieval on the intensity data to generate a phase estimate using an initial diversity function corresponding to the intensity data, generating a phase map from the phase retrieval phase estimate, decomposing the phase map to generate a decomposition vector, generating an updated diversity function by combining the initial diversity function with the decomposition vector, generating an updated estimate of the phase of the optical system by removing the initial diversity function from the phase map. The method may further include repeating the process beginning with iteratively performing a phase retrieval on the intensity data using the updated estimate of the phase of the optical system in place of the initial estimate of the phase of the optical system, and using the updated diversity function in place of the initial diversity function, until a predetermined convergence is achieved.

Retrieval of past and future positive and negative autobiographical experiences.

PubMed

García-Bajos, Elvira; Migueles, Malen

2017-09-01

We studied retrieval-induced forgetting for past or future autobiographical experiences. In the study phase, participants were given cues to remember past autobiographical experiences or to think about experiences that may occur in the future. In both conditions, half of the experiences were positive and half negative. In the retrieval-practice phase, for past and future experiences, participants retrieved either half of the positive or negative experiences using cued recall, or capitals of the world (control groups). Retrieval practice produced recall facilitation and enhanced memory for the practised positive and negative past and future experiences. While retrieval practice on positive experiences did not impair the recall of other positive experiences, we found inhibition for negative past and future experiences when participants practised negative experiences. Furthermore, retrieval practice on positive future experiences inhibited negative future experiences. These positivity biases for autobiographical memory may have practical implications for treatment of emotional disorders.

TMS evidence for a selective role of the precuneus in source memory retrieval.

PubMed

Bonnì, Sonia; Veniero, Domenica; Mastropasqua, Chiara; Ponzo, Viviana; Caltagirone, Carlo; Bozzali, Marco; Koch, Giacomo

2015-04-01

The posteromedial cortex including the precuneus (PC) is thought to be involved in episodic memory retrieval. Here we used continuous theta burst stimulation (cTBS) to disentangle the role of the precuneus in the recognition memory process in a sample of healthy subjects. During the encoding phase, subjects were presented with a series of colored pictures. Afterwards, during the retrieval phase, all previously presented items and a sample of new pictures were presented in black, and subjects were asked to indicate whether each item was new or old, and in the latter case to indicate the associated color. cTBS was delivered over PC, posterior parietal cortex (PPC) and vertex before the retrieval phase. The data were analyzed in terms of hits, false alarms, source errors and omissions. cTBS over the precuneus, but not over the PPC or the vertex, induced a selective decrease in source memory errors, indicating an improvement in context retrieval. All the other accuracy measurements were unchanged. These findings suggest a direct implication of the precuneus in successful context-dependent retrieval. Copyright © 2015 Elsevier B.V. All rights reserved.

Extended capture range for focus-diverse phase retrieval in segmented aperture systems using geometrical optics.

PubMed

Jurling, Alden S; Fienup, James R

2014-03-01

Extending previous work by Thurman on wavefront sensing for segmented-aperture systems, we developed an algorithm for estimating segment tips and tilts from multiple point spread functions in different defocused planes. We also developed methods for overcoming two common modes for stagnation in nonlinear optimization-based phase retrieval algorithms for segmented systems. We showed that when used together, these methods largely solve the capture range problem in focus-diverse phase retrieval for segmented systems with large tips and tilts. Monte Carlo simulations produced a rate of success better than 98% for the combined approach.

Penalized maximum likelihood reconstruction for x-ray differential phase-contrast tomography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brendel, Bernhard, E-mail: bernhard.brendel@philips.com; Teuffenbach, Maximilian von; Noël, Peter B.

2016-01-15

Purpose: The purpose of this work is to propose a cost function with regularization to iteratively reconstruct attenuation, phase, and scatter images simultaneously from differential phase contrast (DPC) acquisitions, without the need of phase retrieval, and examine its properties. Furthermore this reconstruction method is applied to an acquisition pattern that is suitable for a DPC tomographic system with continuously rotating gantry (sliding window acquisition), overcoming the severe smearing in noniterative reconstruction. Methods: We derive a penalized maximum likelihood reconstruction algorithm to directly reconstruct attenuation, phase, and scatter image from the measured detector values of a DPC acquisition. The proposed penaltymore » comprises, for each of the three images, an independent smoothing prior. Image quality of the proposed reconstruction is compared to images generated with FBP and iterative reconstruction after phase retrieval. Furthermore, the influence between the priors is analyzed. Finally, the proposed reconstruction algorithm is applied to experimental sliding window data acquired at a synchrotron and results are compared to reconstructions based on phase retrieval. Results: The results show that the proposed algorithm significantly increases image quality in comparison to reconstructions based on phase retrieval. No significant mutual influence between the proposed independent priors could be observed. Further it could be illustrated that the iterative reconstruction of a sliding window acquisition results in images with substantially reduced smearing artifacts. Conclusions: Although the proposed cost function is inherently nonconvex, it can be used to reconstruct images with less aliasing artifacts and less streak artifacts than reconstruction methods based on phase retrieval. Furthermore, the proposed method can be used to reconstruct images of sliding window acquisitions with negligible smearing artifacts.« less

Grid workflow validation using ontology-based tacit knowledge: A case study for quantitative remote sensing applications

NASA Astrophysics Data System (ADS)

Liu, Jia; Liu, Longli; Xue, Yong; Dong, Jing; Hu, Yingcui; Hill, Richard; Guang, Jie; Li, Chi

2017-01-01

Workflow for remote sensing quantitative retrieval is the ;bridge; between Grid services and Grid-enabled application of remote sensing quantitative retrieval. Workflow averts low-level implementation details of the Grid and hence enables users to focus on higher levels of application. The workflow for remote sensing quantitative retrieval plays an important role in remote sensing Grid and Cloud computing services, which can support the modelling, construction and implementation of large-scale complicated applications of remote sensing science. The validation of workflow is important in order to support the large-scale sophisticated scientific computation processes with enhanced performance and to minimize potential waste of time and resources. To research the semantic correctness of user-defined workflows, in this paper, we propose a workflow validation method based on tacit knowledge research in the remote sensing domain. We first discuss the remote sensing model and metadata. Through detailed analysis, we then discuss the method of extracting the domain tacit knowledge and expressing the knowledge with ontology. Additionally, we construct the domain ontology with Protégé. Through our experimental study, we verify the validity of this method in two ways, namely data source consistency error validation and parameters matching error validation.

Phase retrieval for crystalline specimens

NASA Astrophysics Data System (ADS)

Arnal, Romain A.; Millane, Rick P.

2017-09-01

The recent availability of ultra-bright and ultra-short X-rays pulses from new sources called x-ray free-electron lasers (XFELs) has introduced a new paradigm in X-ray crystallography. Called "diffraction-before-destruction," this paradigm addresses the main problems that plague crystallography using synchrotron sources. However, the phase problem of coherent diffraction imaging remains: one has to retrieve the phase of the measured diffraction amplitude in order to reconstruct the object. Fibrous and membrane proteins that crystallize in 1D and 2D crystals can now potentially be used for data collection with free-electron lasers. The crystallographic phase problem with such crystalline specimens is eased as the Fourier amplitude can be sampled more finely than at the Bragg sampling along one or two directions. Here we characterise uniqueness of the phase problem for different types of crystalline specimen. Simulated ab initio phase retrieval using iterative projection algorithms for 2D crystals is presented.

Investigation of the Iterative Phase Retrieval Algorithm for Interferometric Applications

NASA Astrophysics Data System (ADS)

Gombkötő, Balázs; Kornis, János

2010-04-01

Sequentially recorded intensity patterns reflected from a coherently illuminated diffuse object can be used to reconstruct the complex amplitude of the scattered beam. Several iterative phase retrieval algorithms are known in the literature to obtain the initially unknown phase from these longitudinally displaced intensity patterns. When two sequences are recorded in two different states of a centimeter sized object in optical setups that are similar to digital holographic interferometry-but omitting the reference wave-, displacement, deformation, or shape measurement is theoretically possible. To do this, the retrieved phase pattern should contain information not only about the intensities and locations of the point sources of the object surface, but their relative phase as well. Not only experiments require strict mechanical precision to record useful data, but even in simulations several parameters influence the capabilities of iterative phase retrieval, such as object to camera distance range, uniform or varying camera step sequence, speckle field characteristics, and sampling. Experiments were done to demonstrate this principle with an as large as 5×5 cm sized deformable object as well. Good initial results were obtained in an imaging setup, where the intensity pattern sequences were recorded near the image plane.

Feasibility study: Assess the feasibility of siting a monitored retrievable storage facility. Phase 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

King, J.W.

1993-08-01

The purpose of phase one of this study are: To understand the waste management system and a monitored retrievable storage facility; and to determine whether the applicant has real interest in pursuing the feasibility assessment process. Contents of this report are: Generating electric power; facts about exposure to radiation; handling storage, and transportation techniques; description of a proposed monitored retrievable storage facility; and benefits to be received by host jurisdiction.

Grating-based x-ray differential phase contrast imaging with twin peaks in phase-stepping curves—phase retrieval and dewrapping

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, Yi; Xie, Huiqiao; Tang, Xiangyang, E-mail: xiangyang.tang@emory.edu

Purpose: X-ray differential phase contrast CT implemented with Talbot interferometry employs phase-stepping to extract information of x-ray attenuation, phase shift, and small-angle scattering. Since inaccuracy may exist in the absorption grating G{sub 2} due to an imperfect fabrication, the effective period of G{sub 2} can be as large as twice the nominal period, leading to a phenomenon of twin peaks that differ remarkably in their heights. In this work, the authors investigate how to retrieve and dewrap the phase signal from the phase-stepping curve (PSC) with the feature of twin peaks for x-ray phase contrast imaging. Methods: Based on themore » paraxial Fresnel–Kirchhoff theory, the analytical formulae to characterize the phenomenon of twin peaks in the PSC are derived. Then an approach to dewrap the retrieved phase signal by jointly using the phases of the first- and second-order Fourier components is proposed. Through an experimental investigation using a prototype x-ray phase contrast imaging system implemented with Talbot interferometry, the authors evaluate and verify the derived analytic formulae and the proposed approach for phase retrieval and dewrapping. Results: According to theoretical analysis, the twin-peak phenomenon in PSC is a consequence of combined effects, including the inaccuracy in absorption grating G{sub 2}, mismatch between phase grating and x-ray source spectrum, and finite size of x-ray tube’s focal spot. The proposed approach is experimentally evaluated by scanning a phantom consisting of organic materials and a lab mouse. The preliminary data show that compared to scanning G{sub 2} over only one single nominal period and correcting the measured phase signal with an intuitive phase dewrapping method that is being used in the field, stepping G{sub 2} over twice its nominal period and dewrapping the measured phase signal with the proposed approach can significantly improve the quality of x-ray differential phase contrast imaging in both radiograph and CT. Conclusions: Using the phase retrieval and dewrapping methods proposed to deal with the phenomenon of twin peaks in PSCs and phase wrapping, the performance of grating-based x-ray differential phase contrast radiography and CT can be significantly improved.« less

Evaluation of X-band polarimetric radar estimation of rainfall and rain drop size distribution parameters in West Africa

NASA Astrophysics Data System (ADS)

Koffi, A. K.; Gosset, M.; Zahiri, E.-P.; Ochou, A. D.; Kacou, M.; Cazenave, F.; Assamoi, P.

2014-06-01

As part of the African Monsoon Multidisciplinary Analysis (AMMA) field campaign an X-band dual-polarization Doppler radar was deployed in Benin, West-Africa, in 2006 and 2007, together with a reinforced rain gauge network and several optical disdrometers. Based on this data set, a comparative study of several rainfall estimators that use X-band polarimetric radar data is presented. In tropical convective systems as encountered in Benin, microwave attenuation by rain is significant and quantitative precipitation estimation (QPE) at X-band is a challenge. Here, several algorithms based on the combined use of reflectivity, differential reflectivity and differential phase shift are evaluated against rain gauges and disdrometers. Four rainfall estimators were tested on twelve rainy events: the use of attenuation corrected reflectivity only (estimator R(ZH)), the use of the specific phase shift only R(KDP), the combination of specific phase shift and differential reflectivity R(KDP,ZDR) and an estimator that uses three radar parameters R(ZH,ZDR,KDP). The coefficients of the power law relationships between rain rate and radar variables were adjusted either based on disdrometer data and simulation, or on radar-gauges observations. The three polarimetric based algorithms with coefficients predetermined on observations outperform the R(ZH) estimator for rain rates above 10 mm/h which explain most of the rainfall in the studied region. For the highest rain rates (above 30 mm/h) R(KDP) shows even better scores, and given its performances and its simplicity of implementation, is recommended. The radar based retrieval of two parameters of the rain drop size distribution, the normalized intercept parameter NW and the volumetric median diameter Dm was evaluated on four rainy days thanks to disdrometers. The frequency distributions of the two parameters retrieved by the radar are very close to those observed with the disdrometer. NW retrieval based on a combination of ZH-KDP-ZDR works well whatever the a priori assumption made on the drop shapes. Dm retrieval based on ZDR alone performs well, but if satisfactory ZDR measurements are not available, the combination ZH-KDP provides satisfactory results for both Dm and NW if an appropriate a priori assumption on drop shape is made.

Iterative-Transform Phase Retrieval Using Adaptive Diversity

NASA Technical Reports Server (NTRS)

Dean, Bruce H.

2007-01-01

A phase-diverse iterative-transform phase-retrieval algorithm enables high spatial-frequency, high-dynamic-range, image-based wavefront sensing. [The terms phase-diverse, phase retrieval, image-based, and wavefront sensing are defined in the first of the two immediately preceding articles, Broadband Phase Retrieval for Image-Based Wavefront Sensing (GSC-14899-1).] As described below, no prior phase-retrieval algorithm has offered both high dynamic range and the capability to recover high spatial-frequency components. Each of the previously developed image-based phase-retrieval techniques can be classified into one of two categories: iterative transform or parametric. Among the modifications of the original iterative-transform approach has been the introduction of a defocus diversity function (also defined in the cited companion article). Modifications of the original parametric approach have included minimizing alternative objective functions as well as implementing a variety of nonlinear optimization methods. The iterative-transform approach offers the advantage of ability to recover low, middle, and high spatial frequencies, but has disadvantage of having a limited dynamic range to one wavelength or less. In contrast, parametric phase retrieval offers the advantage of high dynamic range, but is poorly suited for recovering higher spatial frequency aberrations. The present phase-diverse iterative transform phase-retrieval algorithm offers both the high-spatial-frequency capability of the iterative-transform approach and the high dynamic range of parametric phase-recovery techniques. In implementation, this is a focus-diverse iterative-transform phaseretrieval algorithm that incorporates an adaptive diversity function, which makes it possible to avoid phase unwrapping while preserving high-spatial-frequency recovery. The algorithm includes an inner and an outer loop (see figure). An initial estimate of phase is used to start the algorithm on the inner loop, wherein multiple intensity images are processed, each using a different defocus value. The processing is done by an iterative-transform method, yielding individual phase estimates corresponding to each image of the defocus-diversity data set. These individual phase estimates are combined in a weighted average to form a new phase estimate, which serves as the initial phase estimate for either the next iteration of the iterative-transform method or, if the maximum number of iterations has been reached, for the next several steps, which constitute the outerloop portion of the algorithm. The details of the next several steps must be omitted here for the sake of brevity. The overall effect of these steps is to adaptively update the diversity defocus values according to recovery of global defocus in the phase estimate. Aberration recovery varies with differing amounts as the amount of diversity defocus is updated in each image; thus, feedback is incorporated into the recovery process. This process is iterated until the global defocus error is driven to zero during the recovery process. The amplitude of aberration may far exceed one wavelength after completion of the inner-loop portion of the algorithm, and the classical iterative transform method does not, by itself, enable recovery of multi-wavelength aberrations. Hence, in the absence of a means of off-loading the multi-wavelength portion of the aberration, the algorithm would produce a wrapped phase map. However, a special aberration-fitting procedure can be applied to the wrapped phase data to transfer at least some portion of the multi-wavelength aberration to the diversity function, wherein the data are treated as known phase values. In this way, a multiwavelength aberration can be recovered incrementally by successively applying the aberration-fitting procedure to intermediate wrapped phase maps. During recovery, as more of the aberration is transferred to the diversity function following successive iterations around the ter loop, the estimated phase ceases to wrap in places where the aberration values become incorporated as part of the diversity function. As a result, as the aberration content is transferred to the diversity function, the phase estimate resembles that of a reference flat.

Qualification of a Null Lens Using Image-Based Phase Retrieval

NASA Technical Reports Server (NTRS)

Bolcar, Matthew R.; Aronstein, David L.; Hill, Peter C.; Smith, J. Scott; Zielinski, Thomas P.

2012-01-01

In measuring the figure error of an aspheric optic using a null lens, the wavefront contribution from the null lens must be independently and accurately characterized in order to isolate the optical performance of the aspheric optic alone. Various techniques can be used to characterize such a null lens, including interferometry, profilometry and image-based methods. Only image-based methods, such as phase retrieval, can measure the null-lens wavefront in situ - in single-pass, and at the same conjugates and in the same alignment state in which the null lens will ultimately be used - with no additional optical components. Due to the intended purpose of a Dull lens (e.g., to null a large aspheric wavefront with a near-equal-but-opposite spherical wavefront), characterizing a null-lens wavefront presents several challenges to image-based phase retrieval: Large wavefront slopes and high-dynamic-range data decrease the capture range of phase-retrieval algorithms, increase the requirements on the fidelity of the forward model of the optical system, and make it difficult to extract diagnostic information (e.g., the system F/#) from the image data. In this paper, we present a study of these effects on phase-retrieval algorithms in the context of a null lens used in component development for the Climate Absolute Radiance and Refractivity Observatory (CLARREO) mission. Approaches for mitigation are also discussed.

Treatment of Proper Name Retrieval Deficits in an Individual with Temporal Lobe Epilepsy

ERIC Educational Resources Information Center

Minkina, Irene; Ojemann, Jeffrey G.; Grabowski, Thomas J.; Silkes, JoAnn P.; Phatak, Vaishali; Kendall, Diane L.

2013-01-01

Purpose: Studies investigating language deficits in individuals with left temporal-lobe epilepsy have consistently demonstrated impairments in proper name retrieval. The aim of this Phase I rehabilitation study was to investigate the effects of a linguistically distributed word retrieval treatment on proper name retrieval in an individual with…

Cloud-Scale Vertical Velocity and Turbulent Dissipation Rate Retrievals

DOE Data Explorer

Shupe, Matthew

2013-05-22

Time-height fields of retrieved in-cloud vertical wind velocity and turbulent dissipation rate, both retrieved primarily from vertically-pointing, Ka-band cloud radar measurements. Files are available for manually-selected, stratiform, mixed-phase cloud cases observed at the North Slope of Alaska (NSA) site during periods covering the Mixed-Phase Arctic Cloud Experiment (MPACE, late September through early November 2004) and the Indirect and Semi-Direct Aerosol Campaign (ISDAC, April-early May 2008). These time periods will be expanded in a future submission.

X-Ray Phase Imaging for Breast Cancer Detection

DTIC Science & Technology

2010-09-01

regularization seeks the minimum- norm , least squares solution for phase retrieval. The retrieval result with Tikhonov regularization is still unsatisfactory...of norm , that can effectively reflect the accuracy of the retrieved data as an image, if ‖δ Ik+1−δ Ik‖ is less than a predefined threshold value β...pointed out that the proper norm for images is the total variation (TV) norm , which is the L1 norm of the gradient of the image function, and not the

Highly noise-tolerant hybrid algorithm for phase retrieval from a single-shot spatial carrier fringe pattern

NASA Astrophysics Data System (ADS)

Dong, Zhichao; Cheng, Haobo

2018-01-01

A highly noise-tolerant hybrid algorithm (NTHA) is proposed in this study for phase retrieval from a single-shot spatial carrier fringe pattern (SCFP), which effectively combines the merits of spatial carrier phase shift method and two dimensional continuous wavelet transform (2D-CWT). NTHA firstly extracts three phase-shifted fringe patterns from the SCFP with one pixel malposition; then calculates phase gradients by subtracting the reference phase from the other two target phases, which are retrieved respectively from three phase-shifted fringe patterns by 2D-CWT; finally, reconstructs the phase map by a least square gradient integration method. Its typical characters include but not limited to: (1) doesn't require the spatial carrier to be constant; (2) the subtraction mitigates edge errors of 2D-CWT; (3) highly noise-tolerant, because not only 2D-CWT is noise-insensitive, but also the noise in the fringe pattern doesn't directly take part in the phase reconstruction as in previous hybrid algorithm. Its feasibility and performances are validated extensively by simulations and contrastive experiments to temporal phase shift method, Fourier transform and 2D-CWT methods.

Single grating x-ray imaging for dynamic biological systems

NASA Astrophysics Data System (ADS)

Morgan, Kaye S.; Paganin, David M.; Parsons, David W.; Donnelley, Martin; Yagi, Naoto; Uesugi, Kentaro; Suzuki, Yoshio; Takeuchi, Akihisa; Siu, Karen K. W.

2012-07-01

Biomedical studies are already benefiting from the excellent contrast offered by phase contrast x-ray imaging, but live imaging work presents several challenges. Living samples make it particularly difficult to achieve high resolution, sensitive phase contrast images, as exposures must be short and cannot be repeated. We therefore present a single-exposure, high-flux method of differential phase contrast imaging [1, 2, 3] in the context of imaging live airways for Cystic Fibrosis (CF) treatment assessment [4]. The CF study seeks to non-invasively observe the liquid lining the airways, which should increase in depth in response to effective treatments. Both high spatial resolution and sensitivity are required in order to track micron size changes in a liquid that is not easily differentiated from the tissue on which it lies. Our imaging method achieves these goals by using a single attenuation grating or grid as a reference pattern, and analyzing how the sample deforms the pattern to quantitatively retrieve the phase depth of the sample. The deformations are mapped at each pixel in the image using local cross-correlations comparing each 'sample and pattern' image with a reference 'pattern only' image taken before the sample is introduced. This produces a differential phase image, which may be integrated to give the sample phase depth.

Phase retrieval with the transport-of-intensity equation in an arbitrarily-shaped aperture by iterative discrete cosine transforms

DOE Office of Scientific and Technical Information (OSTI.GOV)

Huang, Lei; Zuo, Chao; Idir, Mourad

A novel transport-of-intensity equation (TIE) based phase retrieval method is proposed with putting an arbitrarily-shaped aperture into the optical wavefield. In this arbitrarily-shaped aperture, the TIE can be solved under non-uniform illuminations and even non-homogeneous boundary conditions by iterative discrete cosine transforms with a phase compensation mechanism. Simulation with arbitrary phase, arbitrary aperture shape, and non-uniform intensity distribution verifies the effective compensation and high accuracy of the proposed method. Experiment is also carried out to check the feasibility of the proposed method in real measurement. Comparing to the existing methods, the proposed method is applicable for any types of phasemore » distribution under non-uniform illumination and non-homogeneous boundary conditions within an arbitrarily-shaped aperture, which enables the technique of TIE with hard aperture become a more flexible phase retrieval tool in practical measurements.« less

Phase retrieval with the transport-of-intensity equation in an arbitrarily-shaped aperture by iterative discrete cosine transforms

DOE PAGES

Huang, Lei; Zuo, Chao; Idir, Mourad; ...

2015-04-21

A novel transport-of-intensity equation (TIE) based phase retrieval method is proposed with putting an arbitrarily-shaped aperture into the optical wavefield. In this arbitrarily-shaped aperture, the TIE can be solved under non-uniform illuminations and even non-homogeneous boundary conditions by iterative discrete cosine transforms with a phase compensation mechanism. Simulation with arbitrary phase, arbitrary aperture shape, and non-uniform intensity distribution verifies the effective compensation and high accuracy of the proposed method. Experiment is also carried out to check the feasibility of the proposed method in real measurement. Comparing to the existing methods, the proposed method is applicable for any types of phasemore » distribution under non-uniform illumination and non-homogeneous boundary conditions within an arbitrarily-shaped aperture, which enables the technique of TIE with hard aperture become a more flexible phase retrieval tool in practical measurements.« less

Measurement of phase function of aerosol at different altitudes by CCD Lidar

NASA Astrophysics Data System (ADS)

Sun, Peiyu; Yuan, Ke'e.; Yang, Jie; Hu, Shunxing

2018-02-01

The aerosols near the ground are closely related to human health and climate change, the study on which has important significance. As we all know, the aerosol is inhomogeneous at different altitudes, of which the phase function is also different. In order to simplify the retrieval algorithm, it is usually assumed that the aerosol is uniform at different altitudes, which will bring measurement error. In this work, an experimental approach is demonstrated to measure the scattering phase function of atmospheric aerosol particles at different heights by CCD lidar system, which could solve the problem of the traditional CCD lidar system in assumption of phase function. The phase functions obtained by the new experimental approach are used to retrieve the aerosol extinction coefficient profiles. By comparison of the aerosol extinction coefficient retrieved by Mie-scattering aerosol lidar and CCD lidar at night, the reliability of new experimental approach is verified.

Three-step interferometric method with blind phase shifts by use of interframe correlation between interferograms

NASA Astrophysics Data System (ADS)

Muravsky, Leonid I.; Kmet', Arkady B.; Stasyshyn, Ihor V.; Voronyak, Taras I.; Bobitski, Yaroslav V.

2018-06-01

A new three-step interferometric method with blind phase shifts to retrieve phase maps (PMs) of smooth and low-roughness engineering surfaces is proposed. Evaluating of two unknown phase shifts is fulfilled by using the interframe correlation between interferograms. The method consists of two stages. The first stage provides recording of three interferograms of a test object and their processing including calculation of unknown phase shifts, and retrieval of a coarse PM. The second stage implements firstly separation of high-frequency and low-frequency PMs and secondly producing of a fine PM consisting of areal surface roughness and waviness PMs. Extraction of the areal surface roughness and waviness PMs is fulfilled by using a linear low-pass filter. The computer simulation and experiments fulfilled to retrieve a gauge block surface area and its areal surface roughness and waviness have confirmed the reliability of the proposed three-step method.

Retrieving cloud, dust and ozone abundances in the Martian atmosphere using SPICAM/UV nadir spectra

NASA Astrophysics Data System (ADS)

Willame, Y.; Vandaele, A. C.; Depiesse, C.; Lefèvre, F.; Letocart, V.; Gillotay, D.; Montmessin, F.

2017-08-01

We present the retrieval algorithm developed to analyse nadir spectra from SPICAM/UV aboard Mars-Express. The purpose is to retrieve simultaneously several parameters of the Martian atmosphere and surface: the dust optical depth, the ozone total column, the cloud opacity and the surface albedo. The retrieval code couples the use of an existing complete radiative transfer code, an inversion method and a cloud detection algorithm. We describe the working principle of our algorithm and the parametrisation used to model the required absorption, scattering and reflection processes of the solar UV radiation that occur in the Martian atmosphere and at its surface. The retrieval method has been applied on 4 Martian years of SPICAM/UV data to obtain climatologies of the different quantities under investigation. An overview of the climatology is given for each species showing their seasonal and spatial distributions. The results show a good qualitative agreement with previous observations. Quantitative comparisons of the retrieved dust optical depths indicate generally larger values than previous studies. Possible shortcomings in the dust modelling (altitude profile) have been identified and may be part of the reason for this difference. The ozone results are found to be influenced by the presence of clouds. Preliminary quantitative comparisons show that our retrieved ozone columns are consistent with other results when no ice clouds are present, and are larger for the cases with clouds at high latitude. Sensitivity tests have also been performed showing that the use of other a priori assumptions such as the altitude distribution or some scattering properties can have an important impact on the retrieval.

56. The Role of Prefrontal Cortex in Self-Referential Memory Retrieval in Schizophrenia

PubMed Central

Jimenez, Amy; Lee, Junghee; Wynn, Jonathan K.; Horan, William; Iglesias, Julio; Hoy, Jennifer; Green, Michael F.

2017-01-01

Abstract Background: Enhanced memory for self-oriented information is known as the self-referential memory (SRM) effect. fMRI studies of the SRM effect have largely focused on encoding, revealing selective engagement of medial prefrontal cortex (mPFC) during “self” relative to other semantic processing conditions. Other areas typically activated during self-processing include the ventrolateral prefrontal cortex (vlPFC) and temporo-parietal junction (TPJ). Previous imaging work by our group indicated that patients with schizophrenia activate regions similar to controls during encoding of self-referential information. However, little is known about activation patterns during retrieval, or how activation during encoding relates to retrieval behaviorally. The current study utilized an SRM task to examine: (1) the neural correlates of the retrieval of previously encoded self-oriented information, and (2) the relationship between behavioral data from the retrieval phase and fMRI data at encoding. Methods: 20 clinically stable schizophrenia outpatients and 16 demographically matched healthy controls completed an SRM task modified for event-related fMRI. During the encoding phase, trait adjectives were judged in terms of structural features (“case” condition), social desirability (“other” condition), or as self-referential (“self” condition). Following a 12-minute delay comprised of distractor tasks, memory for trait adjectives was tested during an unexpected yes–no recognition test (retrieval phase). Voxel-wise whole-brain BOLD signal analysis of retrieval phase data was used to examine contrasts of interest with a cluster-threshold of Z = 2.3, P < .05, corrected for multiple comparisons. Results: During retrieval, both groups demonstrated better recognition discriminability (d-prime) for adjectives from the “self” and “other” conditions compared to the “case” condition; d-prime scores were greater for the “self” condition compared to the “other” condition at the trend level. During retrieval, controls showed greater activation than patients in several areas of lateral prefrontal cortex including inferior frontal gyrus (Brodmann Area, BA, 44/45) and middle frontal gyrus (BA 9) for words from the “self” condition. Further, level of activation of mPFC (BA 10) during encoding was positively correlated with d-prime for the “self” condition in controls, but not patients. Conclusion: Although the groups demonstrated comparable behavioral performance during the retrieval phase of an SRM task, regional BOLD activation of prefrontal regions discriminated patients from controls during the retrieval of self-oriented information. The current findings add to a growing body of literature highlighting the critical role of disrupted mPFC activity in self-oriented processing in schizophrenia.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Montanini, R.; Freni, F.; Rossi, G. L.

This paper reports one of the first experimental results on the application of ultrasound activated lock-in vibrothermography for quantitative assessment of buried flaws in complex cast parts. The use of amplitude modulated ultrasonic heat generation allowed selective response of defective areas within the part, as the defect itself is turned into a local thermal wave emitter. Quantitative evaluation of hidden damages was accomplished by estimating independently both the area and the depth extension of the buried flaws, while x-ray 3D computed tomography was used as reference for sizing accuracy assessment. To retrieve flaw's area, a simple yet effective histogram-based phasemore » image segmentation algorithm with automatic pixels classification has been developed. A clear correlation was found between the thermal (phase) signature measured by the infrared camera on the target surface and the actual mean cross-section area of the flaw. Due to the very fast cycle time (<30 s/part), the method could potentially be applied for 100% quality control of casting components.« less

[Modeling continuous scaling of NDVI based on fractal theory].

PubMed

Luan, Hai-Jun; Tian, Qing-Jiu; Yu, Tao; Hu, Xin-Li; Huang, Yan; Du, Ling-Tong; Zhao, Li-Min; Wei, Xi; Han, Jie; Zhang, Zhou-Wei; Li, Shao-Peng

2013-07-01

Scale effect was one of the very important scientific problems of remote sensing. The scale effect of quantitative remote sensing can be used to study retrievals' relationship between different-resolution images, and its research became an effective way to confront the challenges, such as validation of quantitative remote sensing products et al. Traditional up-scaling methods cannot describe scale changing features of retrievals on entire series of scales; meanwhile, they are faced with serious parameters correction issues because of imaging parameters' variation of different sensors, such as geometrical correction, spectral correction, etc. Utilizing single sensor image, fractal methodology was utilized to solve these problems. Taking NDVI (computed by land surface radiance) as example and based on Enhanced Thematic Mapper Plus (ETM+) image, a scheme was proposed to model continuous scaling of retrievals. Then the experimental results indicated that: (a) For NDVI, scale effect existed, and it could be described by fractal model of continuous scaling; (2) The fractal method was suitable for validation of NDVI. All of these proved that fractal was an effective methodology of studying scaling of quantitative remote sensing.

Advantages of phase retrieval for fast x-ray tomographic microscopy

NASA Astrophysics Data System (ADS)

Mokso, R.; Marone, F.; Irvine, S.; Nyvlt, M.; Schwyn, D.; Mader, K.; Taylor, G. K.; Krapp, H. G.; Skeren, M.; Stampanoni, M.

2013-12-01

In near-field imaging with partially coherent x-rays, the phase shifting properties of the sample are encoded in the diffraction fringes that appear as an additional intensity modulation in the x-ray projection images. These Fresnel fringes are often regarded as purely an enhancement of the visibility at the interfaces. We show that retrieving the phase information contained in these patterns significantly advances the developments in fast micro-tomography. Improving temporal resolution without intensifying radiation damage implies a shortening of the exposure time rather than increasing the photon flux on the sample. Phase retrieval, to a large extent, compensates the consequent photon count moderation in the images, by fully exploiting the stronger refraction effect as compared with absorption. Two single-distance phase retrieval methods are evaluated for the case of an in situ 3 Hz micro-tomography of a rapidly evolving liquid foam, and an in vivo 6 Hz micro-tomography of a blowfly. A new dual-detector setup is introduced for simultaneous acquisition of two near-field diffraction patterns. Our goal is to couple high temporal, spatial and density resolution in a single imaging system in a dose-efficient manner, opening further options for dynamic four-dimensional studies.

Development of Dual-Retrieval Processes in Recall: Learning, Forgetting, and Reminiscence

ERIC Educational Resources Information Center

Brainerd, C. J.; Aydin, C.; Reyna, V. F.

2012-01-01

We investigated the development of dual-retrieval processes with a low-burden paradigm that is suitable for research with children and neurocognitively impaired populations (e.g., older adults with mild cognitive impairment or dementia). Rich quantitative information can be obtained about recollection, reconstruction, and familiarity judgment by…

Preparations for Global Precipitation Measurement(GPM)Ground Validation

NASA Technical Reports Server (NTRS)

Bidwell, S. W.; Bibyk, I. K.; Duming, J. F.; Everett, D. F.; Smith, E. A.; Wolff, D. B.

2004-01-01

The Global Precipitation Measurement (GPM) program is an international partnership led by the National Aeronautics and Space Administration (NASA) and the Japan Aerospace Exploration Agency (JAXA). GPM will improve climate, weather, and hydro-meterorological forecasts through more frequent and more accurate measurement of precipitation across the globe. This paper describes the concept and the preparations for Ground Validation within the GPM program. Ground Validation (GV) plays a critical role in the program by investigating and quantitatively assessing the errors within the satellite retrievals. These quantitative estimates of retrieval errors will assist the scientific community by bounding the errors within their research products. The two fundamental requirements of the GPM Ground Validation program are: (1) error characterization of the precipitation retrievals and (2) continual improvement of the satellite retrieval algorithms. These two driving requirements determine the measurements, instrumentation, and location for ground observations. This paper describes GV plans for estimating the systematic and random components of retrieval error and for characterizing the spatial and temporal structure of the error. This paper describes the GPM program for algorithm improvement in which error models are developed and experimentally explored to uncover the physical causes of errors within the retrievals. GPM will ensure that information gained through Ground Validation is applied to future improvements in the spaceborne retrieval algorithms. This paper discusses the potential locations for validation measurement and research, the anticipated contributions of GPM's international partners, and the interaction of Ground Validation with other GPM program elements.

Validation and Refinement of a Lunar Irradiance Model for Suomi NPP VIIRS Day-Night Band Quantitative Nighttime Applications

NASA Astrophysics Data System (ADS)

Miller, S. D.; Combs, C.; Wagner, S.; Viticchiè, B.; Walther, A.; Solbrig, J.

2014-12-01

The VIIRS Day-Night Band provides the first calibrated observations of nocturnal low-light visible/near-infrared (~500-900 nm response, 710 nm central wavelength) radiances, including reflected moonlight down to values of 3 × 10-5 W·m-2·sr-1. These novel measurements afford the first opportunity to attempt nighttime retrievals of optical depth for optically thick clouds when moonlight is available, thereby advancing our ability to observe the diurnal cycle of such structures as marine stratocumuli which are thought to play an important role in determining climate and climate feedbacks. In order to leverage the Day-Night Band measurements in this capacity, we must first convert the upwelling top-of-atmosphere radiances to equivalent values of reflectance. Doing so requires a detailed knowledge of the down-welling top-of-atmosphere lunar spectral irradiance which, unlike sunlight, varies significantly over the course of the ~29.5 day lunar cycle. This research summarizes the ongoing development, validation, and refinement of a lunar irradiance model designed to convert Day-Night Band radiances to equivalent lunar reflectance. Comparisons between daytime and nighttime Day-Night Band reflectance for vicarious calibration targets offering radiometric stability (e.g., White Sands, Salar de Uyuni, Dome-C, and snow fields) confirms the model's performance to within an expected ~10% uncertainty. An observed lunar-phase-dependent trend associated with the model's assumption of a disk-averaged albedo was addressed via analysis of a version of the model adapted for comparison against Meteosat Second Generation SEVIRI lunar measurements. The analysis resulted in a phase-dependent 6th order polynomial correction to the model and expected model uncertainty improvements to within ~5%. Examples of lunar reflectance imagery for operational applications and the provisional quantitative application of Day-Night Band lunar reflectance to nighttime cloud optical property retrievals, bearing relevance to the diurnally resolved global climate data record, are shown.

Subsampling phase retrieval for rapid thermal measurements of heated microstructures.

PubMed

Taylor, Lucas N; Talghader, Joseph J

2016-07-15

A subsampling technique for real-time phase retrieval of high-speed thermal signals is demonstrated with heated metal lines such as those found in microelectronic interconnects. The thermal signals were produced by applying a current through aluminum resistors deposited on soda-lime-silica glass, and the resulting refractive index changes were measured using a Mach-Zehnder interferometer with a microscope objective and high-speed camera. The temperatures of the resistors were measured both by the phase-retrieval method and by monitoring the resistance of the aluminum lines. The method used to analyze the phase is at least 60× faster than the state of the art but it maintains a small spatial phase noise of 16 nm, remaining comparable to the state of the art. For slowly varying signals, the system is able to perform absolute phase measurements over time, distinguishing temperature changes as small as 2 K. With angular scanning or structured illumination improvements, the system could also perform fast thermal tomography.

Web image retrieval using an effective topic and content-based technique

NASA Astrophysics Data System (ADS)

Lee, Ching-Cheng; Prabhakara, Rashmi

2005-03-01

There has been an exponential growth in the amount of image data that is available on the World Wide Web since the early development of Internet. With such a large amount of information and image available and its usefulness, an effective image retrieval system is thus greatly needed. In this paper, we present an effective approach with both image matching and indexing techniques that improvise on existing integrated image retrieval methods. This technique follows a two-phase approach, integrating query by topic and query by example specification methods. In the first phase, The topic-based image retrieval is performed by using an improved text information retrieval (IR) technique that makes use of the structured format of HTML documents. This technique consists of a focused crawler that not only provides for the user to enter the keyword for the topic-based search but also, the scope in which the user wants to find the images. In the second phase, we use query by example specification to perform a low-level content-based image match in order to retrieve smaller and relatively closer results of the example image. From this, information related to the image feature is automatically extracted from the query image. The main objective of our approach is to develop a functional image search and indexing technique and to demonstrate that better retrieval results can be achieved.

Grid-Independent Compressive Imaging and Fourier Phase Retrieval

ERIC Educational Resources Information Center

Liao, Wenjing

2013-01-01

This dissertation is composed of two parts. In the first part techniques of band exclusion(BE) and local optimization(LO) are proposed to solve linear continuum inverse problems independently of the grid spacing. The second part is devoted to the Fourier phase retrieval problem. Many situations in optics, medical imaging and signal processing call…

Single-intensity-recording optical encryption technique based on phase retrieval algorithm and QR code

NASA Astrophysics Data System (ADS)

Wang, Zhi-peng; Zhang, Shuai; Liu, Hong-zhao; Qin, Yi

2014-12-01

Based on phase retrieval algorithm and QR code, a new optical encryption technology that only needs to record one intensity distribution is proposed. In this encryption process, firstly, the QR code is generated from the information to be encrypted; and then the generated QR code is placed in the input plane of 4-f system to have a double random phase encryption. For only one intensity distribution in the output plane is recorded as the ciphertext, the encryption process is greatly simplified. In the decryption process, the corresponding QR code is retrieved using phase retrieval algorithm. A priori information about QR code is used as support constraint in the input plane, which helps solve the stagnation problem. The original information can be recovered without distortion by scanning the QR code. The encryption process can be implemented either optically or digitally, and the decryption process uses digital method. In addition, the security of the proposed optical encryption technology is analyzed. Theoretical analysis and computer simulations show that this optical encryption system is invulnerable to various attacks, and suitable for harsh transmission conditions.

Novel Fourier-domain constraint for fast phase retrieval in coherent diffraction imaging.

PubMed

Latychevskaia, Tatiana; Longchamp, Jean-Nicolas; Fink, Hans-Werner

2011-09-26

Coherent diffraction imaging (CDI) for visualizing objects at atomic resolution has been realized as a promising tool for imaging single molecules. Drawbacks of CDI are associated with the difficulty of the numerical phase retrieval from experimental diffraction patterns; a fact which stimulated search for better numerical methods and alternative experimental techniques. Common phase retrieval methods are based on iterative procedures which propagate the complex-valued wave between object and detector plane. Constraints in both, the object and the detector plane are applied. While the constraint in the detector plane employed in most phase retrieval methods requires the amplitude of the complex wave to be equal to the squared root of the measured intensity, we propose a novel Fourier-domain constraint, based on an analogy to holography. Our method allows achieving a low-resolution reconstruction already in the first step followed by a high-resolution reconstruction after further steps. In comparison to conventional schemes this Fourier-domain constraint results in a fast and reliable convergence of the iterative reconstruction process. © 2011 Optical Society of America

Halo-free phase contrast microscopy (Conference Presentation)

NASA Astrophysics Data System (ADS)

Nguyen, Tan H.; Kandel, Mikhail E.; Shakir, Haadi M.; Best, Catherine; Do, Minh N.; Popescu, Gabriel

2017-02-01

The phase contrast (PC) method is one of the most impactful developments in the four-century long history of microscopy. It allows for intrinsic, nondestructive contrast of transparent specimens, such as live cells. However, PC is plagued by the halo artifact, a result of insufficient spatial coherence in the illumination field, which limits its applicability. We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data for reversing the halo artifact. Measuring four independent intensity images, our approach first measures haloed phase maps of the sample. We solve for the halo-free sample transmission function by using a physical model of the image formation under partial spatial coherence. Using this halo-free sample transmission, we can numerically generate artifact-free PC images. Furthermore, this transmission can be further used to obtain quantitative information about the sample, e.g., the thickness with known refractive indices, dry mass of live cells during their cycles. We tested our hfPC method on various control samples, e.g., beads, pillars and validated its potential for biological investigation by imaging live HeLa cells, red blood cells, and neurons.

Changes in the modulation of brain activity during context encoding vs. context retrieval across the adult lifespan.

PubMed

Ankudowich, E; Pasvanis, S; Rajah, M N

2016-10-01

Age-related deficits in context memory may arise from neural changes underlying both encoding and retrieval of context information. Although age-related functional changes in the brain regions supporting context memory begin at midlife, little is known about the functional changes with age that support context memory encoding and retrieval across the adult lifespan. We investigated how age-related functional changes support context memory across the adult lifespan by assessing linear changes with age during successful context encoding and retrieval. Using functional magnetic resonance imaging (fMRI), we compared young, middle-aged and older adults during both encoding and retrieval of spatial and temporal details of faces. Multivariate behavioral partial least squares (B-PLS) analysis of fMRI data identified a pattern of whole-brain activity that correlated with a linear age term and a pattern of whole-brain activity that was associated with an age-by-memory phase (encoding vs. retrieval) interaction. Further investigation of this latter effect identified three main findings: 1) reduced phase-related modulation in bilateral fusiform gyrus, left superior/anterior frontal gyrus and right inferior frontal gyrus that started at midlife and continued to older age, 2) reduced phase-related modulation in bilateral inferior parietal lobule that occurred only in older age, and 3) changes in phase-related modulation in older but not younger adults in left middle frontal gyrus and bilateral parahippocampal gyrus that was indicative of age-related over-recruitment. We conclude that age-related reductions in context memory arise in midlife and are related to changes in perceptual recollection and changes in fronto-parietal retrieval monitoring. Crown Copyright © 2016. Published by Elsevier Inc. All rights reserved.

Phase imaging using shifted wavefront sensor images.

PubMed

Zhang, Zhengyun; Chen, Zhi; Rehman, Shakil; Barbastathis, George

2014-11-01

We propose a new approach to the complete retrieval of a coherent field (amplitude and phase) using the same hardware configuration as a Shack-Hartmann sensor but with two modifications: first, we add a transversally shifted measurement to resolve ambiguities in the measured phase; and second, we employ factored form descent (FFD), an inverse algorithm for coherence retrieval, with a hard rank constraint. We verified the proposed approach using both numerical simulations and experiments.

Broadband CARS spectral phase retrieval using a time-domain Kramers–Kronig transform

PubMed Central

Liu, Yuexin; Lee, Young Jong; Cicerone, Marcus T.

2014-01-01

We describe a closed-form approach for performing a Kramers–Kronig (KK) transform that can be used to rapidly and reliably retrieve the phase, and thus the resonant imaginary component, from a broadband coherent anti-Stokes Raman scattering (CARS) spectrum with a nonflat background. In this approach we transform the frequency-domain data to the time domain, perform an operation that ensures a causality criterion is met, then transform back to the frequency domain. The fact that this method handles causality in the time domain allows us to conveniently account for spectrally varying nonresonant background from CARS as a response function with a finite rise time. A phase error accompanies KK transform of data with finite frequency range. In examples shown here, that phase error leads to small (<1%) errors in the retrieved resonant spectra. PMID:19412273

3D-shape of objects with straight line-motion by simultaneous projection of color coded patterns

NASA Astrophysics Data System (ADS)

Flores, Jorge L.; Ayubi, Gaston A.; Di Martino, J. Matías; Castillo, Oscar E.; Ferrari, Jose A.

2018-05-01

In this work, we propose a novel technique to retrieve the 3D shape of dynamic objects by the simultaneous projection of a fringe pattern and a homogeneous light pattern which are both coded in two of the color channels of a RGB image. The fringe pattern, red channel, is used to retrieve the phase by phase-shift algorithms with arbitrary phase-step, while the homogeneous pattern, blue channel, is used to match pixels from the test object in consecutive images, which are acquired at different positions, and thus, to determine the speed of the object. The proposed method successfully overcomes the standard requirement of projecting fringes of two different frequencies; one frequency to extract object information and the other one to retrieve the phase. Validation experiments are presented.

Frequency and causes of failed MODIS cloud property retrievals for liquid phase clouds over global oceans.

PubMed

Cho, Hyoun-Myoung; Zhang, Zhibo; Meyer, Kerry; Lebsock, Matthew; Platnick, Steven; Ackerman, Andrew S; Di Girolamo, Larry; C-Labonnote, Laurent; Cornet, Céline; Riedi, Jerome; Holz, Robert E

2015-05-16

Moderate Resolution Imaging Spectroradiometer (MODIS) retrieves cloud droplet effective radius ( r e ) and optical thickness ( τ ) by projecting observed cloud reflectances onto a precomputed look-up table (LUT). When observations fall outside of the LUT, the retrieval is considered "failed" because no combination of τ and r e within the LUT can explain the observed cloud reflectances. In this study, the frequency and potential causes of failed MODIS retrievals for marine liquid phase (MLP) clouds are analyzed based on 1 year of Aqua MODIS Collection 6 products and collocated CALIOP and CloudSat observations. The retrieval based on the 0.86 µm and 2.1 µm MODIS channel combination has an overall failure rate of about 16% (10% for the 0.86 µm and 3.7 µm combination). The failure rates are lower over stratocumulus regimes and higher over the broken trade wind cumulus regimes. The leading type of failure is the " r e too large" failure accounting for 60%-85% of all failed retrievals. The rest is mostly due to the " r e too small" or τ retrieval failures. Enhanced retrieval failure rates are found when MLP cloud pixels are partially cloudy or have high subpixel inhomogeneity, are located at special Sun-satellite viewing geometries such as sunglint, large viewing or solar zenith angles, or cloudbow and glory angles, or are subject to cloud masking, cloud overlapping, and/or cloud phase retrieval issues. The majority (more than 84%) of failed retrievals along the CALIPSO track can be attributed to at least one or more of these potential reasons. The collocated CloudSat radar reflectivity observations reveal that the remaining failed retrievals are often precipitating. It remains an open question whether the extremely large r e values observed in these clouds are the consequence of true cloud microphysics or still due to artifacts not included in this study.

Frequency and causes of failed MODIS cloud property retrievals for liquid phase clouds over global oceans

PubMed Central

Cho, Hyoun‐Myoung; Meyer, Kerry; Lebsock, Matthew; Platnick, Steven; Ackerman, Andrew S.; Di Girolamo, Larry; C.‐Labonnote, Laurent; Cornet, Céline; Riedi, Jerome; Holz, Robert E.

2015-01-01

Abstract Moderate Resolution Imaging Spectroradiometer (MODIS) retrieves cloud droplet effective radius (r e) and optical thickness (τ) by projecting observed cloud reflectances onto a precomputed look‐up table (LUT). When observations fall outside of the LUT, the retrieval is considered “failed” because no combination of τ and r e within the LUT can explain the observed cloud reflectances. In this study, the frequency and potential causes of failed MODIS retrievals for marine liquid phase (MLP) clouds are analyzed based on 1 year of Aqua MODIS Collection 6 products and collocated CALIOP and CloudSat observations. The retrieval based on the 0.86 µm and 2.1 µm MODIS channel combination has an overall failure rate of about 16% (10% for the 0.86 µm and 3.7 µm combination). The failure rates are lower over stratocumulus regimes and higher over the broken trade wind cumulus regimes. The leading type of failure is the “r e too large” failure accounting for 60%–85% of all failed retrievals. The rest is mostly due to the “r e too small” or τ retrieval failures. Enhanced retrieval failure rates are found when MLP cloud pixels are partially cloudy or have high subpixel inhomogeneity, are located at special Sun‐satellite viewing geometries such as sunglint, large viewing or solar zenith angles, or cloudbow and glory angles, or are subject to cloud masking, cloud overlapping, and/or cloud phase retrieval issues. The majority (more than 84%) of failed retrievals along the CALIPSO track can be attributed to at least one or more of these potential reasons. The collocated CloudSat radar reflectivity observations reveal that the remaining failed retrievals are often precipitating. It remains an open question whether the extremely large r e values observed in these clouds are the consequence of true cloud microphysics or still due to artifacts not included in this study. PMID:27656330

Experimental determination of pore shapes using phase retrieval from q -space NMR diffraction

NASA Astrophysics Data System (ADS)

Demberg, Kerstin; Laun, Frederik Bernd; Bertleff, Marco; Bachert, Peter; Kuder, Tristan Anselm

2018-05-01

This paper presents an approach to solving the phase problem in nuclear magnetic resonance (NMR) diffusion pore imaging, a method that allows imaging the shape of arbitrary closed pores filled with an NMR-detectable medium for investigation of the microstructure of biological tissue and porous materials. Classical q -space imaging composed of two short diffusion-encoding gradient pulses yields, analogously to diffraction experiments, the modulus squared of the Fourier transform of the pore image which entails an inversion problem: An unambiguous reconstruction of the pore image requires both magnitude and phase. Here the phase information is recovered from the Fourier modulus by applying a phase retrieval algorithm. This allows omitting experimentally challenging phase measurements using specialized temporal gradient profiles. A combination of the hybrid input-output algorithm and the error reduction algorithm was used with dynamically adapting support (shrinkwrap extension). No a priori knowledge on the pore shape was fed to the algorithm except for a finite pore extent. The phase retrieval approach proved successful for simulated data with and without noise and was validated in phantom experiments with well-defined pores using hyperpolarized xenon gas.

Experimental determination of pore shapes using phase retrieval from q-space NMR diffraction.

PubMed

Demberg, Kerstin; Laun, Frederik Bernd; Bertleff, Marco; Bachert, Peter; Kuder, Tristan Anselm

2018-05-01

This paper presents an approach to solving the phase problem in nuclear magnetic resonance (NMR) diffusion pore imaging, a method that allows imaging the shape of arbitrary closed pores filled with an NMR-detectable medium for investigation of the microstructure of biological tissue and porous materials. Classical q-space imaging composed of two short diffusion-encoding gradient pulses yields, analogously to diffraction experiments, the modulus squared of the Fourier transform of the pore image which entails an inversion problem: An unambiguous reconstruction of the pore image requires both magnitude and phase. Here the phase information is recovered from the Fourier modulus by applying a phase retrieval algorithm. This allows omitting experimentally challenging phase measurements using specialized temporal gradient profiles. A combination of the hybrid input-output algorithm and the error reduction algorithm was used with dynamically adapting support (shrinkwrap extension). No a priori knowledge on the pore shape was fed to the algorithm except for a finite pore extent. The phase retrieval approach proved successful for simulated data with and without noise and was validated in phantom experiments with well-defined pores using hyperpolarized xenon gas.

Phase retrieval by constrained power inflation and signum flipping

NASA Astrophysics Data System (ADS)

Laganà, A. R.; Morabito, A. F.; Isernia, T.

2016-12-01

In this paper we consider the problem of retrieving a signal from the modulus of its Fourier transform (or other suitable transformations) and some additional information, which is also known as "Phase Retrieval" problem. The problem arises in many areas of applied Sciences such as optics, electron microscopy, antennas, and crystallography. In particular, we introduce a new approach, based on power inflation and tunneling, allowing an increased robustness with respect to the possible occurrence of false solutions. Preliminary results are presented for the simple yet relevant case of one-dimensional arrays and noisy data.

Phase retrieval based wavefront sensing experimental implementation and wavefront sensing accuracy calibration

NASA Astrophysics Data System (ADS)

Mao, Heng; Wang, Xiao; Zhao, Dazun

2009-05-01

As a wavefront sensing (WFS) tool, Baseline algorithm, which is classified as the iterative-transform algorithm of phase retrieval, estimates the phase distribution at pupil from some known PSFs at defocus planes. By using multiple phase diversities and appropriate phase unwrapping methods, this algorithm can accomplish reliable unique solution and high dynamic phase measurement. In the paper, a Baseline algorithm based wavefront sensing experiment with modification of phase unwrapping has been implemented, and corresponding Graphical User Interfaces (GUI) software has also been given. The adaptability and repeatability of Baseline algorithm have been validated in experiments. Moreover, referring to the ZYGO interferometric results, the WFS accuracy of this algorithm has been exactly calibrated.

4-D cloud properties from passive satellite data and applications to resolve the flight icing threat to aircraft

NASA Astrophysics Data System (ADS)

Smith, William L., Jr.

The threat for aircraft icing in clouds is a significant hazard that routinely impacts aviation operations. Accurate diagnoses and forecasts of aircraft icing conditions requires identifying the location and vertical distribution of clouds with super-cooled liquid water (SLW) droplets, as well as the characteristics of the droplet size distribution. Traditional forecasting methods rely on guidance from numerical models and conventional observations, neither of which currently resolve cloud properties adequately on the optimal scales needed for aviation. Satellite imagers provide measurements over large areas with high spatial resolution that can be interpreted to identify the locations and characteristics of clouds, including features associated with adverse weather and storms. This thesis develops new techniques for interpreting cloud products derived from satellite data to infer the flight icing threat to aircraft in a wide range of cloud conditions. For unobscured low clouds, the icing threat is determined using empirical relationships developed from correlations between satellite imager retrievals of liquid water path and droplet size with icing conditions reported by pilots (PIREPS). For deep ice over water cloud systems, ice and liquid water content profiles are derived by using the imager cloud properties to constrain climatological information on cloud vertical structure and water phase obtained apriori from radar and lidar observations, and from cloud model analyses. Retrievals of the SLW content embedded within overlapping clouds are mapped to the icing threat using guidance from an airfoil modeling study. Compared to PIREPS, the satellite icing detection and intensity accuracies are found to be about 90% and 70%, respectively. Mean differences between the imager IWC retrievals with those from CloudSat and Calipso are less than 30%. This level of closure in the cloud water budget can only be achieved by correcting for errors in the imager retrievals due to the simplifying but poor assumption that deep optically thick clouds are single-phase and vertically homogeneous. When applied to geostationary satellite data, the profiling method provides a real-time characterization of clouds in 4-D. This research should improve the utility of satellite imager data for quantitatively diagnosing and predicting clouds and their effects in weather and climate applications.

Robust phase recovery in temporal speckle pattern interferometry using a 3D directional wavelet transform.

PubMed

Federico, Alejandro; Kaufmann, Guillermo H

2009-08-01

We propose an approach based on a 3D directional wavelet transform to retrieve optical phase distributions in temporal speckle pattern interferometry. We show that this approach can effectively recover phase distributions in time series of speckle interferograms that are affected by sets of adjacent nonmodulated pixels. The performance of this phase retrieval approach is analyzed by introducing a temporal carrier in the out-of-plane interferometer setup and assuming modulation loss and noise effects. The advantages and limitations of this approach are finally discussed.

Retrieval-Induced vs. Context-Induced Forgetting: Does Retrieval-Induced Forgetting Depend on Context Shifts?

PubMed Central

Soares, Julia S.; Polack, Cody W.; Miller, Ralph R.

2015-01-01

Retrieval-induced forgetting (RIF) is the observation that retrieval of target information causes forgetting of related non-target information. A number of accounts of this phenomenon have been proposed, including a context-shift based account (Jonker, Seli, & Macleod, 2013). This account proposes that RIF occurs due to the context shift from study to retrieval practice, provided there is little context shift between retrieval practice and test phases. We tested both claims put forth by this context account. In Experiment 1, we degraded the context shift between study and retrieval practice by implementing a generative study condition that was highly similar to retrieval practice. We observed no degradation of RIF for these generated exemplars relative to a conventional study control. In Experiment 2, we conceptually replicated the finding of RIF following generative study, and tested whether context differences between each of the three phases affected the size of RIF. Our findings were again contrary to the predictions of the context account. Conjointly, the two experiments refute arguments about the potential inadequacy of our context shifts that could be used to explain either result alone. Overall, our results are most consistent with an inhibitory account of RIF (e.g., Anderson, 2003). PMID:26389628

Ground-based remote sensing of thin clouds in the Arctic

NASA Astrophysics Data System (ADS)

Garrett, T. J.; Zhao, C.

2012-11-01

This paper describes a method for using interferometer measurements of downwelling thermal radiation to retrieve the properties of single-layer clouds. Cloud phase is determined from ratios of thermal emission in three "micro-windows" where absorption by water vapor is particularly small. Cloud microphysical and optical properties are retrieved from thermal emission in two micro-windows, constrained by the transmission through clouds of stratospheric ozone emission. Assuming a cloud does not approximate a blackbody, the estimated 95% confidence retrieval errors in effective radius, visible optical depth, number concentration, and water path are, respectively, 10%, 20%, 38% (55% for ice crystals), and 16%. Applied to data from the Atmospheric Radiation Measurement program (ARM) North Slope of Alaska - Adjacent Arctic Ocean (NSA-AAO) site near Barrow, Alaska, retrievals show general agreement with ground-based microwave radiometer measurements of liquid water path. Compared to other retrieval methods, advantages of this technique include its ability to characterize thin clouds year round, that water vapor is not a primary source of retrieval error, and that the retrievals of microphysical properties are only weakly sensitive to retrieved cloud phase. The primary limitation is the inapplicability to thicker clouds that radiate as blackbodies.

"BRAIN": Baruch Retrieval of Automated Information for Negotiations.

ERIC Educational Resources Information Center

Levenstein, Aaron, Ed.

1981-01-01

A data processing program that can be used as a research and collective bargaining aid for colleges is briefly described and the fields of the system are outlined. The system, known as BRAIN (Baruch Retrieval of Automated Information for Negotiations), is designed primarily as an instrument for quantitative and qualitative analysis. BRAIN consists…

Deficient Contractor Business Systems: Applying the Value at Risk (VAR) Model to Earned Value Management Systems

DTIC Science & Technology

2013-06-01

measuring numerical risk to the government ( Galway , 2004). However, quantitative risk analysis is rarely utilized in DoD acquisition programs because the...quantitative assessment of the EVMS itself. Galway (2004) practically linked project quantitative risk assessment to EVM by focusing on cost...Kindle version]. Retrieved from Amazon.com 83 Galway , L. (2004, February). Quantitative risk analysis for project management: A critical review

Waveform Retrieval and Phase Identification for Seismic Data from the CASS Experiment

NASA Astrophysics Data System (ADS)

Li, Zhiwei; You, Qingyu; Ni, Sidao; Hao, Tianyao; Wang, Hongti; Zhuang, Cantao

2013-05-01

The little destruction to the deployment site and high repeatability of the Controlled Accurate Seismic Source (CASS) shows its potential for investigating seismic wave velocities in the Earth's crust. However, the difficulty in retrieving impulsive seismic waveforms from the CASS data and identifying the seismic phases substantially prevents its wide applications. For example, identification of the seismic phases and accurate measurement of travel times are essential for resolving the spatial distribution of seismic velocities in the crust. Until now, it still remains a challenging task to estimate the accurate travel times of different seismic phases from the CASS data which features extended wave trains, unlike processing of the waveforms from impulsive events such as earthquakes or explosive sources. In this study, we introduce a time-frequency analysis method to process the CASS data, and try to retrieve the seismic waveforms and identify the major seismic phases traveling through the crust. We adopt the Wigner-Ville Distribution (WVD) approach which has been used in signal detection and parameter estimation for linear frequency modulation (LFM) signals, and proves to feature the best time-frequency convergence capability. The Wigner-Hough transform (WHT) is applied to retrieve the impulsive waveforms from multi-component LFM signals, which comprise seismic phases with different arrival times. We processed the seismic data of the 40-ton CASS in the field experiment around the Xinfengjiang reservoir with the WVD and WHT methods. The results demonstrate that these methods are effective in waveform retrieval and phase identification, especially for high frequency seismic phases such as PmP and SmS with strong amplitudes in large epicenter distance of 80-120 km. Further studies are still needed to improve the accuracy on travel time estimation, so as to further promote applicability of the CASS for and imaging the seismic velocity structure.

Phase retrieval of images using Gaussian radial bases.

PubMed

Trahan, Russell; Hyland, David

2013-12-20

Here, the possibility of a noniterative solution to the phase retrieval problem is explored. A new look is taken at the phase retrieval problem that reveals that knowledge of a diffraction pattern's frequency components is enough to recover the image without projective iterations. This occurs when the image is formed using Gaussian bases that give the convenience of a continuous Fourier transform existing in a compact form where square pixels do not. The Gaussian bases are appropriate when circular apertures are used to detect the diffraction pattern because of their optical transfer functions, as discussed briefly. An algorithm is derived that is capable of recovering an image formed by Gaussian bases from only the Fourier transform's modulus, without background constraints. A practical example is shown.

Enhanced x-ray imaging for a thin film cochlear implant with metal artefacts using phase retrieval tomography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arhatari, B. D.; ARC Centre of Excellence for Coherent X-ray Science, Melbourne; Harris, A. R.

Phase retrieval tomography has been successfully used to enhance imaging in systems that exhibit poor absorption contrast. However, when highly absorbing regions are present in a sample, so-called metal artefacts can appear in the tomographic reconstruction. We demonstrate that straightforward approaches for metal artefact reconstruction, developed in absorption contrast tomography, can be applied when using phase retrieval. Using a prototype thin film cochlear implant that has high and low absorption components made from iridium (or platinum) and plastic, respectively, we show that segmentation of the various components is possible and hence measurement of the electrode geometry and relative location tomore » other regions of interest can be achieved.« less

Phase recovery in temporal speckle pattern interferometry using the generalized S-transform.

PubMed

Federico, Alejandro; Kaufmann, Guillermo H

2008-04-15

We propose a novel approach based on the generalized S-transform to retrieve optical phase distributions in temporal speckle pattern interferometry. The performance of the proposed approach is compared with those given by well-known techniques based on the continuous wavelet, the Hilbert transforms, and a smoothed time-frequency distribution by analyzing interferometric data degraded by noise, nonmodulating pixels, and modulation loss. The advantages and limitations of the proposed phase retrieval approach are discussed.

A protocol for searching the most probable phase-retrieved maps in coherent X-ray diffraction imaging by exploiting the relationship between convergence of the retrieved phase and success of calculation.

PubMed

Sekiguchi, Yuki; Hashimoto, Saki; Kobayashi, Amane; Oroguchi, Tomotaka; Nakasako, Masayoshi

2017-09-01

Coherent X-ray diffraction imaging (CXDI) is a technique for visualizing the structures of non-crystalline particles with size in the submicrometer to micrometer range in material sciences and biology. In the structural analysis of CXDI, the electron density map of a specimen particle projected along the direction of the incident X-rays can be reconstructed only from the diffraction pattern by using phase-retrieval (PR) algorithms. However, in practice, the reconstruction, relying entirely on the computational procedure, sometimes fails because diffraction patterns miss the data in small-angle regions owing to the beam stop and saturation of the detector pixels, and are modified by Poisson noise in X-ray detection. To date, X-ray free-electron lasers have allowed us to collect a large number of diffraction patterns within a short period of time. Therefore, the reconstruction of correct electron density maps is the bottleneck for efficiently conducting structure analyses of non-crystalline particles. To automatically address the correctness of retrieved electron density maps, a data analysis protocol to extract the most probable electron density maps from a set of maps retrieved from 1000 different random seeds for a single diffraction pattern is proposed. Through monitoring the variations of the phase values during PR calculations, the tendency for the PR calculations to succeed when the retrieved phase sets converged on a certain value was found. On the other hand, if the phase set was in persistent variation, the PR calculation tended to fail to yield the correct electron density map. To quantify this tendency, here a figure of merit for the variation of the phase values during PR calculation is introduced. In addition, a PR protocol to evaluate the similarity between a map of the highest figure of merit and other independently reconstructed maps is proposed. The protocol is implemented and practically examined in the structure analyses for diffraction patterns from aggregates of gold colloidal particles. Furthermore, the feasibility of the protocol in the structure analysis of organelles from biological cells is examined.

Evaluating cloud retrieval algorithms with the ARM BBHRP framework

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mlawer,E.; Dunn,M.; Mlawer, E.

2008-03-10

Climate and weather prediction models require accurate calculations of vertical profiles of radiative heating. Although heating rate calculations cannot be directly validated due to the lack of corresponding observations, surface and top-of-atmosphere measurements can indirectly establish the quality of computed heating rates through validation of the calculated irradiances at the atmospheric boundaries. The ARM Broadband Heating Rate Profile (BBHRP) project, a collaboration of all the working groups in the program, was designed with these heating rate validations as a key objective. Given the large dependence of radiative heating rates on cloud properties, a critical component of BBHRP radiative closure analysesmore » has been the evaluation of cloud microphysical retrieval algorithms. This evaluation is an important step in establishing the necessary confidence in the continuous profiles of computed radiative heating rates produced by BBHRP at the ARM Climate Research Facility (ACRF) sites that are needed for modeling studies. This poster details the continued effort to evaluate cloud property retrieval algorithms within the BBHRP framework, a key focus of the project this year. A requirement for the computation of accurate heating rate profiles is a robust cloud microphysical product that captures the occurrence, height, and phase of clouds above each ACRF site. Various approaches to retrieve the microphysical properties of liquid, ice, and mixed-phase clouds have been processed in BBHRP for the ACRF Southern Great Plains (SGP) and the North Slope of Alaska (NSA) sites. These retrieval methods span a range of assumptions concerning the parameterization of cloud location, particle density, size, shape, and involve different measurement sources. We will present the radiative closure results from several different retrieval approaches for the SGP site, including those from Microbase, the current 'reference' retrieval approach in BBHRP. At the NSA, mixed-phase clouds and cloud with a low optical depth are prevalent; the radiative closure studies using Microbase demonstrated significant residuals. As an alternative to Microbase at NSA, the Shupe-Turner cloud property retrieval algorithm, aimed at improving the partitioning of cloud phase and incorporating more constrained, conditional microphysics retrievals, also has been evaluated using the BBHRP data set.« less

Information Retrieval and Criticality in Parity-Time-Symmetric Systems.

PubMed

Kawabata, Kohei; Ashida, Yuto; Ueda, Masahito

2017-11-10

By investigating information flow between a general parity-time (PT-)symmetric non-Hermitian system and an environment, we find that the complete information retrieval from the environment can be achieved in the PT-unbroken phase, whereas no information can be retrieved in the PT-broken phase. The PT-transition point thus marks the reversible-irreversible criticality of information flow, around which many physical quantities such as the recurrence time and the distinguishability between quantum states exhibit power-law behavior. Moreover, by embedding a PT-symmetric system into a larger Hilbert space so that the entire system obeys unitary dynamics, we reveal that behind the information retrieval lies a hidden entangled partner protected by PT symmetry. Possible experimental situations are also discussed.

Improvements to GPS Airborne Radio Occultation in the Lower Troposphere Through Implementation of the Phase Matching Method

NASA Astrophysics Data System (ADS)

Wang, K.-N.; Garrison, J. L.; Haase, J. S.; Murphy, B. J.

2017-10-01

Airborne radio occultation (ARO) is a remote sensing technique for atmospheric sounding using Global Positioning System signals received by an airborne instrument. The atmospheric refractivity profile, which depends on pressure, temperature, and water vapor, can be retrieved by measuring the signal delay due to the refractive medium through which the signal traverses. The ARO system was developed to make repeated observations within an individual meteorological event such as a tropical storm, regardless of the presence of clouds and precipitation, and complements existing observation techniques such as dropsondes and satellite remote sensing. RO systems can suffer multipath ray propagation in the lower troposphere if there are strong refractivity gradients, for example, due to a highly variable moisture distribution or a sharp boundary layer, interfering with continuous carrier phase tracking as well as complicating retrievals. The phase matching method has now been adapted for ARO and is shown to reduce negative biases in the refractivity retrieval by providing robust retrievals of bending angle in the presence of multipath. The retrieval results are presented for a flight campaign in September 2010 for Hurricane Karl in the Caribbean Sea. The accuracy is assessed through comparison with the European Centre for Medium Range Weather Forecasts Interim Reanalysis. The fractional difference in refractivity can be maintained at a standard deviation of 2% from flight level down to a height of 2 km. The phase matching method decreases the negative refractivity bias by as much as 4% over the classical geometrical optics retrieval method.

Destination Entry And Retrieval With The Ali-Scout Navigation System Fast-Trac Phase Iib Deliverable

DOT National Transportation Integrated Search

1996-12-01

AFTER TRAINING, 36 DRIVERS RETRIEVED AND ENTERED A TOTAL OF 20 DESTINATIONS USING AN ALI-SCOUT NAVIGATION COMPUTER AND 10 DESTINATIONS USING A TOUCHSCREEN SIMULATION WHILE SITTING IN A VEHICLE MOCKUP. RETRIEVAL INVOLVED KEYING IN PART OF THE DESTINAT...

Plant leaf chlorophyll content retrieval based on a field imaging spectroscopy system.

PubMed

Liu, Bo; Yue, Yue-Min; Li, Ru; Shen, Wen-Jing; Wang, Ke-Lin

2014-10-23

A field imaging spectrometer system (FISS; 380-870 nm and 344 bands) was designed for agriculture applications. In this study, FISS was used to gather spectral information from soybean leaves. The chlorophyll content was retrieved using a multiple linear regression (MLR), partial least squares (PLS) regression and support vector machine (SVM) regression. Our objective was to verify the performance of FISS in a quantitative spectral analysis through the estimation of chlorophyll content and to determine a proper quantitative spectral analysis method for processing FISS data. The results revealed that the derivative reflectance was a more sensitive indicator of chlorophyll content and could extract content information more efficiently than the spectral reflectance, which is more significant for FISS data compared to ASD (analytical spectral devices) data, reducing the corresponding RMSE (root mean squared error) by 3.3%-35.6%. Compared with the spectral features, the regression methods had smaller effects on the retrieval accuracy. A multivariate linear model could be the ideal model to retrieve chlorophyll information with a small number of significant wavelengths used. The smallest RMSE of the chlorophyll content retrieved using FISS data was 0.201 mg/g, a relative reduction of more than 30% compared with the RMSE based on a non-imaging ASD spectrometer, which represents a high estimation accuracy compared with the mean chlorophyll content of the sampled leaves (4.05 mg/g). Our study indicates that FISS could obtain both spectral and spatial detailed information of high quality. Its image-spectrum-in-one merit promotes the good performance of FISS in quantitative spectral analyses, and it can potentially be widely used in the agricultural sector.

Plant Leaf Chlorophyll Content Retrieval Based on a Field Imaging Spectroscopy System

PubMed Central

Liu, Bo; Yue, Yue-Min; Li, Ru; Shen, Wen-Jing; Wang, Ke-Lin

2014-01-01

A field imaging spectrometer system (FISS; 380–870 nm and 344 bands) was designed for agriculture applications. In this study, FISS was used to gather spectral information from soybean leaves. The chlorophyll content was retrieved using a multiple linear regression (MLR), partial least squares (PLS) regression and support vector machine (SVM) regression. Our objective was to verify the performance of FISS in a quantitative spectral analysis through the estimation of chlorophyll content and to determine a proper quantitative spectral analysis method for processing FISS data. The results revealed that the derivative reflectance was a more sensitive indicator of chlorophyll content and could extract content information more efficiently than the spectral reflectance, which is more significant for FISS data compared to ASD (analytical spectral devices) data, reducing the corresponding RMSE (root mean squared error) by 3.3%–35.6%. Compared with the spectral features, the regression methods had smaller effects on the retrieval accuracy. A multivariate linear model could be the ideal model to retrieve chlorophyll information with a small number of significant wavelengths used. The smallest RMSE of the chlorophyll content retrieved using FISS data was 0.201 mg/g, a relative reduction of more than 30% compared with the RMSE based on a non-imaging ASD spectrometer, which represents a high estimation accuracy compared with the mean chlorophyll content of the sampled leaves (4.05 mg/g). Our study indicates that FISS could obtain both spectral and spatial detailed information of high quality. Its image-spectrum-in-one merit promotes the good performance of FISS in quantitative spectral analyses, and it can potentially be widely used in the agricultural sector. PMID:25341439

Automatic Retrieval of Newly Instructed Cue-Task Associations Seen in Task-Conflict Effects in the First Trial after Cue-Task Instructions.

PubMed

Meiran, Nachshon; Pereg, Maayan

2017-01-01

Novel stimulus-response associations are retrieved automatically even without prior practice. Is this true for novel cue-task associations? The experiment involved miniblocks comprising three phases and task switching. In the INSTRUCTION phase, two new stimuli (or familiar cues) were arbitrarily assigned as cues for up-down/right-left tasks performed on placeholder locations. In the UNIVALENT phase, there was no task cue since placeholder's location afforded one task but the placeholders were the stimuli that we assigned as task cues for the following BIVALENT phase (involving target locations affording both tasks). Thus, participants held the novel cue-task associations in memory while executing the UNIVALENT phase. Results show poorer performance in the first univalent trial when the placeholder was associated with the opposite task (incompatible) than when it was compatible, an effect that was numerically larger with newly instructed cues than with familiar cues. These results indicate automatic retrieval of newly instructed cue-task associations.

Phase retrieval in annulus sector domain by non-iterative methods

NASA Astrophysics Data System (ADS)

Wang, Xiao; Mao, Heng; Zhao, Da-zun

2008-03-01

Phase retrieval could be achieved by solving the intensity transport equation (ITE) under the paraxial approximation. For the case of uniform illumination, Neumann boundary condition is involved and it makes the solving process more complicated. The primary mirror is usually designed segmented in the telescope with large aperture, and the shape of a segmented piece is often like an annulus sector. Accordingly, It is necessary to analyze the phase retrieval in the annulus sector domain. Two non-iterative methods are considered for recovering the phase. The matrix method is based on the decomposition of the solution into a series of orthogonalized polynomials, while the frequency filtering method depends on the inverse computation process of ITE. By the simulation, it is found that both methods can eliminate the effect of Neumann boundary condition, save a lot of computation time and recover the distorted phase well. The wavefront error (WFE) RMS can be less than 0.05 wavelength, even when some noise is added.

Spatial-area selective retrieval of multiple object-place associations in a hierarchical cognitive map formed by theta phase coding.

PubMed

Sato, Naoyuki; Yamaguchi, Yoko

2009-06-01

The human cognitive map is known to be hierarchically organized consisting of a set of perceptually clustered landmarks. Patient studies have demonstrated that these cognitive maps are maintained by the hippocampus, while the neural dynamics are still poorly understood. The authors have shown that the neural dynamic "theta phase precession" observed in the rodent hippocampus may be capable of forming hierarchical cognitive maps in humans. In the model, a visual input sequence consisting of object and scene features in the central and peripheral visual fields, respectively, results in the formation of a hierarchical cognitive map for object-place associations. Surprisingly, it is possible for such a complex memory structure to be formed in a few seconds. In this paper, we evaluate the memory retrieval of object-place associations in the hierarchical network formed by theta phase precession. The results show that multiple object-place associations can be retrieved with the initial cue of a scene input. Importantly, according to the wide-to-narrow unidirectional connections among scene units, the spatial area for object-place retrieval can be controlled by the spatial area of the initial cue input. These results indicate that the hierarchical cognitive maps have computational advantages on a spatial-area selective retrieval of multiple object-place associations. Theta phase precession dynamics is suggested as a fundamental neural mechanism of the human cognitive map.

Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime

DOE Office of Scientific and Technical Information (OSTI.GOV)

Krenkel, Martin; Toepperwien, Mareike; Alves, Frauke

X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining andmore » labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data.« less

Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime

DOE PAGES

Krenkel, Martin; Toepperwien, Mareike; Alves, Frauke; ...

2017-06-29

X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining andmore » labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data.« less

Multi-event waveform-retrieved distributed optical fiber acoustic sensor using dual-pulse heterodyne phase-sensitive OTDR.

PubMed

He, Xiangge; Xie, Shangran; Liu, Fei; Cao, Shan; Gu, Lijuan; Zheng, Xiaoping; Zhang, Min

2017-02-01

We demonstrate a novel type of distributed optical fiber acoustic sensor, with the ability to detect and retrieve actual temporal waveforms of multiple vibration events that occur simultaneously at different positions along the fiber. The system is realized via a dual-pulse phase-sensitive optical time-domain reflectometry, and the actual waveform is retrieved by heterodyne phase demodulation. Experimental results show that the system has a background noise level as low as 8.91×10^-4  rad/√Hz with a demodulation signal-to-noise ratio of 49.17 dB at 1 kHz, and can achieve a dynamic range of ∼60  dB at 1 kHz (0.1 to 104 rad) for phase demodulation, as well as a detection frequency range from 20 Hz to 25 kHz.

Every factor helps: Rapid Ptychographic Reconstruction

NASA Astrophysics Data System (ADS)

Nashed, Youssef

2015-03-01

Recent advances in microscopy, specifically higher spatial resolution and data acquisition rates, require faster and more robust phase retrieval reconstruction methods. Ptychography is a phase retrieval technique for reconstructing the complex transmission function of a specimen from a sequence of diffraction patterns in visible light, X-ray, and electron microscopes. As technical advances allow larger fields to be imaged, computational challenges arise for reconstructing the correspondingly larger data volumes. Waiting to postprocess datasets offline results in missed opportunities. Here we present a parallel method for real-time ptychographic phase retrieval. It uses a hybrid parallel strategy to divide the computation between multiple graphics processing units (GPUs). A final specimen reconstruction is then achieved by different techniques to merge sub-dataset results into a single complex phase and amplitude image. Results are shown on a simulated specimen and real datasets from X-ray experiments conducted at a synchrotron light source.

Method for utilizing properties of the sinc(x) function for phase retrieval on nyquist-under-sampled data

NASA Technical Reports Server (NTRS)

Dean, Bruce H. (Inventor); Smith, Jeffrey Scott (Inventor); Aronstein, David L. (Inventor)

2012-01-01

Disclosed herein are systems, methods, and non-transitory computer-readable storage media for simulating propagation of an electromagnetic field, performing phase retrieval, or sampling a band-limited function. A system practicing the method generates transformed data using a discrete Fourier transform which samples a band-limited function f(x) without interpolating or modifying received data associated with the function f(x), wherein an interval between repeated copies in a periodic extension of the function f(x) obtained from the discrete Fourier transform is associated with a sampling ratio Q, defined as a ratio of a sampling frequency to a band-limited frequency, and wherein Q is assigned a value between 1 and 2 such that substantially no aliasing occurs in the transformed data, and retrieves a phase in the received data based on the transformed data, wherein the phase is used as feedback to an optical system.

Parallel interactive retrieval of item and associative information from event memory.

PubMed

Cox, Gregory E; Criss, Amy H

2017-09-01

Memory contains information about individual events (items) and combinations of events (associations). Despite the fundamental importance of this distinction, it remains unclear exactly how these two kinds of information are stored and whether different processes are used to retrieve them. We use both model-independent qualitative properties of response dynamics and quantitative modeling of individuals to address these issues. Item and associative information are not independent and they are retrieved concurrently via interacting processes. During retrieval, matching item and associative information mutually facilitate one another to yield an amplified holistic signal. Modeling of individuals suggests that this kind of facilitation between item and associative retrieval is a ubiquitous feature of human memory. Copyright © 2017 Elsevier Inc. All rights reserved.

Results and Validation of MODIS Aerosol Retrievals Over Land and Ocean

NASA Technical Reports Server (NTRS)

Remer, Lorraine; Einaudi, Franco (Technical Monitor)

2001-01-01

The MODerate Resolution Imaging Spectroradiometer (MODIS) instrument aboard the Terra spacecraft has been retrieving aerosol parameters since late February 2000. Initial qualitative checking of the products showed very promising results including matching of land and ocean retrievals at coastlines. Using AERONET ground-based radiometers as our primary validation tool, we have established quantitative validation as well. Our results show that for most aerosol types, the MODIS products fall within the pre-launch estimated uncertainties. Surface reflectance and aerosol model assumptions appear to be sufficiently accurate for the optical thickness retrieval. Dust provides a possible exception, which may be due to non-spherical effects. Over ocean the MODIS products include information on particle size, and these parameters are also validated with AERONET retrievals.

Results and Validation of MODIS Aerosol Retrievals over Land and Ocean

NASA Technical Reports Server (NTRS)

Remer, L. A.; Kaufman, Y. J.; Tanre, D.; Ichoku, C.; Chu, D. A.; Mattoo, S.; Levy, R.; Martins, J. V.; Li, R.-R.; Einaudi, Franco (Technical Monitor)

2000-01-01

The MODerate Resolution Imaging Spectroradiometer (MODIS) instrument aboard the Terra spacecraft has been retrieving aerosol parameters since late February 2000. Initial qualitative checking of the products showed very promising results including matching of land and ocean retrievals at coastlines. Using AERONET ground-based radiometers as our primary validation tool, we have established quantitative validation as well. Our results show that for most aerosol types, the MODIS products fall within the pre-launch estimated uncertainties. Surface reflectance and aerosol model assumptions appear to be sufficiently accurate for the optical thickness retrieval. Dust provides a possible exception, which may be due to non-spherical effects. Over ocean the MODIS products include information on particle size, and these parameters are also validated with AERONET retrievals.

Phase Retrieval from Modulus Using Homeomorphic Signal Processing and the Complex Cepstrum: An Algorithm for Lightning Protection Systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Clark, G A

2004-06-08

In general, the Phase Retrieval from Modulus problem is very difficult. In this report, we solve the difficult, but somewhat more tractable case in which we constrain the solution to a minimum phase reconstruction. We exploit the real-and imaginary part sufficiency properties of the Fourier and Hilbert Transforms of causal sequences to develop an algorithm for reconstructing spectral phase given only spectral modulus. The algorithm uses homeomorphic signal processing methods with the complex cepstrum. The formal problem of interest is: Given measurements of only the modulus {vert_bar}H(k){vert_bar} (no phase) of the Discrete Fourier Transform (DFT) of a real, finite-length, stable,more » causal time domain signal h(n), compute a minimum phase reconstruction {cflx h}(n) of the signal. Then compute the phase of {cflx h}(n) using a DFT, and exploit the result as an estimate of the phase of h(n). The development of the algorithm is quite involved, but the final algorithm and its implementation are very simple. This work was motivated by a Phase Retrieval from Modulus Problem that arose in LLNL Defense Sciences Engineering Division (DSED) projects in lightning protection for buildings. The measurements are limited to modulus-only spectra from a spectrum analyzer. However, it is desired to perform system identification on the building to compute impulse responses and transfer functions that describe the amount of lightning energy that will be transferred from the outside of the building to the inside. This calculation requires knowledge of the entire signals (both modulus and phase). The algorithm and software described in this report are proposed as an approach to phase retrieval that can be used for programmatic needs. This report presents a brief tutorial description of the mathematical problem and the derivation of the phase retrieval algorithm. The efficacy of the theory is demonstrated using simulated signals that meet the assumptions of the algorithm. We see that for the noiseless case, the reconstructions are extremely accurate. When moderate to heavy simulated white Gaussian noise was added, the algorithm performance remained reasonably robust, especially in the low frequency part of the spectrum, which is the part of most interest for lightning protection. Limitations of the algorithm include the following: (1) It does not account for noise in the given spectral modulus. Fortunately, the lightning protection signals of interest generally have a reasonably high signal-to-noise ratio (SNR). (2) The DFT length N must be even and larger than the length of the nonzero part of the measured signals. These constraints are simple to meet in practice. (3) Regardless of the properties of the actual signal h(n), the phase retrieval results are constrained to have the minimum phase property. In most problems of practical interest, these assumptions are very reasonable and probably valid. They are reasonable assumptions for Lightning Protection applications. Proposed future work includes (a) Evaluating the efficacy of the algorithm with real Lightning Protection signals from programmatic applications, (b) Performing a more rigorous analysis of noise effects, (c) Using the algorithm along with advanced system identification algorithms to estimate impulse responses and transfer functions, (d) Developing algorithms to deal with measured partial (truncated) spectral moduli, and (e) R & D of phase retrieval algorithms that specifically deal with general (not necessarily minimum phase) signals, and noisy spectral moduli.« less

Phase retrieval in generalized optical interferometry systems.

PubMed

Farriss, Wesley E; Fienup, James R; Malhotra, Tanya; Vamivakas, A Nick

2018-02-05

Modal analysis of an optical field via generalized interferometry (GI) is a novel technique that treats said field as a linear superposition of transverse modes and recovers the amplitudes of modal weighting coefficients. We use phase retrieval by nonlinear optimization to recover the phase of these modal weighting coefficients. Information diversity increases the robustness of the algorithm by better constraining the solution. Additionally, multiple sets of random starting phase values assist the algorithm in overcoming local minima. The algorithm was able to recover nearly all coefficient phases for simulated fields consisting of up to 21 superpositioned Hermite Gaussian modes from simulated data and proved to be resilient to shot noise.

Atomic resolved phase map of monolayer MoS2 retrieved by spherical aberration-corrected transport of intensity equation.

PubMed

Zhang, Xiaobin; Oshima, Yoshifumi

2016-10-01

An atomic resolution phase map, which enables us to observe charge distribution or magnetic properties at an atomic scale, has been pointed out to be retrieved by transport of intensity equation (TIE) when taking two atomic-resolved transmission electron microscope (TEM) images of small defocus difference. In this work, we firstly obtained the atomic-resolved phase maps of an exfoliated molybdenum disulfide sheet using spherical aberration-corrected transmission electron microscope. We successfully observed 60° grain boundary of mechanically exfoliated monolayer molybdenum disulfide sheet. The relative phase shift of a single molybdenum atomic column to the column consisting of two sulfur atoms was obtained to be about 0.01 rad on average, which was about half lower than the simulated TIE phase map, indicating that the individual atomic sites can be distinguished qualitatively. The appropriate condition for retrieving atomic-resolved TIE phase maps was briefly discussed. © The Author 2016. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Phase object retrieval through scattering medium

NASA Astrophysics Data System (ADS)

Zhao, Ming; Zhao, Meijing; Wu, Houde; Xu, Wenhai

2018-05-01

Optical imaging through a scattering medium has been an interesting and important research topic, especially in the field of biomedical imaging. However, it is still a challenging task due to strong scattering. This paper proposes to recover the phase object behind the scattering medium from one single-shot speckle intensity image using calibrated transmission matrices (TMs). We construct the forward model as a non-linear mapping, since the intensity image loses the phase information, and then a generalized phase retrieval algorithm is employed to recover the hidden object. Moreover, we show that a phase object can be reconstructed with a small portion of the speckle image captured by the camera. The simulation is performed to demonstrate our scheme and test its performance. Finally, a real experiment is set up, we measure the TMs from the scattering medium, and then use it to reconstruct the hidden object. We show that a phase object of size 32 × 32 is retrieved from 150 × 150 speckle grains, which is only 1/50 of the speckles area. We believe our proposed method can benefit the community of imaging through the scattering medium.

Wavefront metrology for coherent hard X-rays by scanning a microsphere.

PubMed

Skjønsfjell, Eirik Torbjørn Bakken; Chushkin, Yuriy; Zontone, Federico; Patil, Nilesh; Gibaud, Alain; Breiby, Dag W

2016-05-16

Characterization of the wavefront of an X-ray beam is of primary importance for all applications where coherence plays a major role. Imaging techniques based on numerically retrieving the phase from interference patterns are often relying on an a-priori assumption of the wavefront shape. In Coherent X-ray Diffraction Imaging (CXDI) a planar incoming wave field is often assumed for the inversion of the measured diffraction pattern, which allows retrieving the real space image via simple Fourier transformation. It is therefore important to know how reliable the plane wave approximation is to describe the real wavefront. Here, we demonstrate that the quantitative wavefront shape and flux distribution of an X-ray beam used for CXDI can be measured by using a micrometer size metal-coated polymer sphere serving in a similar way as the hole array in a Hartmann wavefront sensor. The method relies on monitoring the shape and center of the scattered intensity distribution in the far field using a 2D area detector while raster-scanning the microsphere with respect to the incoming beam. The reconstructed X-ray wavefront was found to have a well-defined central region of approximately 16 µm diameter and a weaker, asymmetric, intensity distribution extending 30 µm from the beam center. The phase front distortion was primarily spherical with an effective radius of 0.55 m which matches the distance to the last upstream beam-defining slit, and could be accurately represented by Zernike polynomials.

Fundamental aspects of the phase retrieval problem

NASA Astrophysics Data System (ADS)

Ferwerda, H. A.

1980-12-01

A review is given of the fundamental aspects of the phase retrieval problem in optical imaging for one dimension. The phase problem is treated using the fact that the wavefunction in the image-plane is a band-limited entire function of order 1. The ambiguity of the phase reconstruction is formulated in terms of the complex zeros of entire functions. Procedures are given how the relevant zeros might be determined. When the zeros are known one can derive dispersion relations which relate the phase of the wavefunction to the intensity distribution. The phase problem of coherence theory is similar to the previously discussed problem and is briefly touched upon. The extension of the phase problem to two dimensions is not straight-forward and still remains to be solved.

A two-step method for retrieving the longitudinal profile of an electron bunch from its coherent radiation

NASA Astrophysics Data System (ADS)

Pelliccia, Daniele; Sen, Tanaji

2014-11-01

The coherent radiation emitted by an electron bunch provides a diagnostic signal that can be used to estimate its longitudinal distribution. Commonly only the amplitude of the intensity spectrum can be measured and the associated phase must be calculated to obtain the bunch profile. Very recently an iterative method was proposed to retrieve this phase. However ambiguities associated with non-uniqueness of the solution are always present in the phase retrieval procedure. Here we present a method to overcome the ambiguity problem by first performing multiple independent runs of the phase retrieval procedure and then second, sorting the good solutions by means of cross-correlation analysis. Results obtained with simulated bunches of various shapes and experimental measured spectra are presented, discussed and compared with the established Kramers-Kronig method. It is shown that even when the effect of the ambiguities is strong, as is the case for a double peak in the profile, the cross-correlation post-processing is able to filter out unwanted solutions. We show that, unlike the Kramers-Kronig method, the combined approach presented is able to faithfully reconstruct complicated bunch profiles.

Quantitative Microbial Risk Assessment Tutorial: Installation of Software for Watershed Modeling in Support of QMRA

EPA Science Inventory

This tutorial provides instructions for accessing, retrieving, and downloading the following software to install on a host computer in support of Quantitative Microbial Risk Assessment (QMRA) modeling:• SDMProjectBuilder (which includes the Microbial Source Module as part...

Global Precipitation Measurement (GPM) Ground Validation: Plans and Preparations

NASA Technical Reports Server (NTRS)

Schwaller, M.; Bidwell, S.; Durning, F. J.; Smith, E.

2004-01-01

The Global Precipitation Measurement (GPM) program is an international partnership led by the National Aeronautics and Space Administration (NASA) and the Japan Aerospace Exploration Agency (JAXA). GPM will improve climate, weather, and hydro-meteorological forecasts through more frequent and more accurate measurement of precipitation across the globe. This paper describes the concept, the planning, and the preparations for Ground Validation within the GPM program. Ground Validation (GV) plays an important role in the program by investigating and quantitatively assessing the errors within the satellite retrievals. These quantitative estimates of retrieval errors will assist the scientific community by bounding the errors within their research products. The two fundamental requirements of the GPM Ground Validation program are: (1) error characterization of the precipitation retrievals and (2) continual improvement of the satellite retrieval algorithms. These two driving requirements determine the measurements, instrumentation, and location for ground observations. This paper outlines GV plans for estimating the systematic and random components of retrieval error and for characterizing the spatial p d temporal structure of the error and plans for algorithm improvement in which error models are developed and experimentally explored to uncover the physical causes of errors within the retrievals. This paper discusses NASA locations for GV measurements as well as anticipated locations from international GPM partners. NASA's primary locations for validation measurements are an oceanic site at Kwajalein Atoll in the Republic of the Marshall Islands and a continental site in north-central Oklahoma at the U.S. Department of Energy's Atmospheric Radiation Measurement Program site.

Autobiographical memory in Parkinson's disease: a retrieval deficit.

PubMed

Souchay, Celine; Smith, Sarah Jane

2013-09-01

This study examined the effects of providing cues to facilitate autobiographical memory retrieval in Parkinson's disease. Previous findings have shown that individuals with Parkinson's disease retrieve fewer specific autobiographical memories than older adult controls. These findings are clinically significant since the quality of autobiographical memory is linked to identity and sense of self. In the current study, 16 older adults with Parkinson's disease without dementia and 16 matched older adult controls were given 3 min in which to recall autobiographical memories associated with five different time periods and to give each memory a short title. Participants were later asked to retrieve the memories in three phases: firstly in a free recall phase; secondly in response to general cues (time periods) and finally in response to specific cues (the short titles previously given). The number of memories and the quality of the memory (general or specific) was recorded in each condition. Compared with matched older adult controls, the Parkinson's disease group was impaired in retrieving the memories that they had previously given in the free recall phase and in response to general cues. The performance of the group with Parkinson's disease was only equivalent to the older adults when they retrieved memories in response to self-generated cues. The findings are discussed in relation to theories of autobiographical memory and the neuropsychology of Parkinson's disease. © 2013 The British Psychological Society.

Quantitative phase imaging by wide field lensless digital holographic microscope

NASA Astrophysics Data System (ADS)

Adinda-Ougba, A.; Koukourakis, N.; Essaidi, A.; Ger­hardt, N. C.; Hofmann, M. R.

2015-05-01

Wide field, lensless microscopes have been developed for telemedicine and for resource limited setting [1]. They are based on in-line digital holography which is capable to provide amplitude and phase information resulting from numerical reconstruction. The phase information enables achieving axial resolution in the nanometer range. Hence, such microscopes provide a powerful tool to determine three-dimensional topologies of microstructures. In this contribution, a compact, low-cost, wide field, lensless microscope is presented, which is capable of providing topological profiles of microstructures in transparent material. Our setup consist only of two main components: a CMOSsensor chip and a laser diode without any need of a pinhole. We use this very simple setup to record holograms of microobjects. A wide field of view of ~24 mm², and a lateral resolution of ~2 μm are achieved. Moreover, amplitude and phase information are obtained from the numerical reconstruction of the holograms using a phase retrieval algorithm together with the angular spectrum propagation method. Topographic information of highly transparent micro-objects is obtained from the phase data. We evaluate our system by recording holograms of lines with different depths written by a focused laser beam. A reliable characterization of laser written microstructures is crucial for their functionality. Our results show that this system is valuable for determination of topological profiles of microstructures in transparent material.

Observation of Phase Objects by Using an X-ray Microscope with a Foucault Knife-Edge

DOE Office of Scientific and Technical Information (OSTI.GOV)

Watanabe, N.; Sasaya, T.; Imai, Y.

2011-09-09

An x-ray microscope with a zone plate was assembled at the synchrotron radiation source of BL3C, Photon Factory. A Foucault knife-edge was set at the back focal plate of the objective zone plate and phase retrieval was tested by scanning the knife-edge. A preliminary result shows that scanning the knife-edge during exposure was effective for phase retrieval. Phase-contrast tomography was investigated using differential projection images calculated from two Schlieren images with the oppositely oriented knife-edges. Fairly good reconstruction images of polystyrene beads and spores could be obtained.

Interferogram conditioning for improved Fourier analysis and application to X-ray phase imaging by grating interferometry.

PubMed

Montaux-Lambert, Antoine; Mercère, Pascal; Primot, Jérôme

2015-11-02

An interferogram conditioning procedure, for subsequent phase retrieval by Fourier demodulation, is presented here as a fast iterative approach aiming at fulfilling the classical boundary conditions imposed by Fourier transform techniques. Interference fringe patterns with typical edge discontinuities were simulated in order to reveal the edge artifacts that classically appear in traditional Fourier analysis, and were consecutively used to demonstrate the correction efficiency of the proposed conditioning technique. Optimization of the algorithm parameters is also presented and discussed. Finally, the procedure was applied to grating-based interferometric measurements performed in the hard X-ray regime. The proposed algorithm enables nearly edge-artifact-free retrieval of the phase derivatives. A similar enhancement of the retrieved absorption and fringe visibility images is also achieved.

Decomposing the interaction between retention interval and study/test practice: The role of retrievability

PubMed Central

Jang, Yoonhee; Wixted, John T.; Pecher, Diane; Zeelenberg, René; Huber, David E.

2012-01-01

Even without feedback, test practice enhances delayed performance compared to study practice, but the size of the effect is variable across studies. We investigated the benefit of testing, separating initially retrievable items from initially non-retrievable items. In two experiments, an initial test determined item retrievability. Retrievable or non-retrievable items were subsequently presented for repeated study or test practice. Collapsing across items, in Experiment 1, we obtained the typical crossover interaction between retention interval and practice type. For retrievable items, however, the crossover interaction was quantitatively different, with a small study benefit for an immediate test and a larger testing benefit after a delay. For non-retrievable items, there was a large study benefit for an immediate test, but one week later there was no difference between the study and test practice conditions. In Experiment 2, initially non-retrievable items were given additional study followed by either an immediate test or even more additional study, and one week later performance did not differ between the two conditions. These results indicate that the effect size of study/test practice is due to the relative contribution of retrievable and non-retrievable items. PMID:22304454

Junior high school students' cognitive process in solving the developed algebraic problems based on information processing taxonomy model

NASA Astrophysics Data System (ADS)

Purwoko, Saad, Noor Shah; Tajudin, Nor'ain Mohd

2017-05-01

This study aims to: i) develop problem solving questions of Linear Equations System of Two Variables (LESTV) based on levels of IPT Model, ii) explain the level of students' skill of information processing in solving LESTV problems; iii) explain students' skill in information processing in solving LESTV problems; and iv) explain students' cognitive process in solving LESTV problems. This study involves three phases: i) development of LESTV problem questions based on Tessmer Model; ii) quantitative survey method on analyzing students' skill level of information processing; and iii) qualitative case study method on analyzing students' cognitive process. The population of the study was 545 eighth grade students represented by a sample of 170 students of five Junior High Schools in Hilir Barat Zone, Palembang (Indonesia) that were chosen using cluster sampling. Fifteen students among them were drawn as a sample for the interview session with saturated information obtained. The data were collected using the LESTV problem solving test and the interview protocol. The quantitative data were analyzed using descriptive statistics, while the qualitative data were analyzed using the content analysis. The finding of this study indicated that students' cognitive process was just at the step of indentifying external source and doing algorithm in short-term memory fluently. Only 15.29% students could retrieve type A information and 5.88% students could retrieve type B information from long-term memory. The implication was the development problems of LESTV had validated IPT Model in modelling students' assessment by different level of hierarchy.

Information Retrieval and Criticality in Parity-Time-Symmetric Systems

NASA Astrophysics Data System (ADS)

Kawabata, Kohei; Ashida, Yuto; Ueda, Masahito

2017-11-01

By investigating information flow between a general parity-time (P T -)symmetric non-Hermitian system and an environment, we find that the complete information retrieval from the environment can be achieved in the P T -unbroken phase, whereas no information can be retrieved in the P T -broken phase. The P T -transition point thus marks the reversible-irreversible criticality of information flow, around which many physical quantities such as the recurrence time and the distinguishability between quantum states exhibit power-law behavior. Moreover, by embedding a P T -symmetric system into a larger Hilbert space so that the entire system obeys unitary dynamics, we reveal that behind the information retrieval lies a hidden entangled partner protected by P T symmetry. Possible experimental situations are also discussed.

Phase-Retrieval Uncertainty Estimation and Algorithm Comparison for the JWST-ISIM Test Campaign

NASA Technical Reports Server (NTRS)

Aronstein, David L.; Smith, J. Scott

2016-01-01

Phase retrieval, the process of determining the exitpupil wavefront of an optical instrument from image-plane intensity measurements, is the baseline methodology for characterizing the wavefront for the suite of science instruments (SIs) in the Integrated Science Instrument Module (ISIM) for the James Webb Space Telescope (JWST). JWST is a large, infrared space telescope with a 6.5-meter diameter primary mirror. JWST is currently NASA's flagship mission and will be the premier space observatory of the next decade. ISIM contains four optical benches with nine unique instruments, including redundancies. ISIM was characterized at the Goddard Space Flight Center (GSFC) in Greenbelt, MD in a series of cryogenic vacuum tests using a telescope simulator. During these tests, phase-retrieval algorithms were used to characterize the instruments. The objective of this paper is to describe the Monte-Carlo simulations that were used to establish uncertainties (i.e., error bars) for the wavefronts of the various instruments in ISIM. Multiple retrieval algorithms were used in the analysis of ISIM phase-retrieval focus-sweep data, including an iterativetransform algorithm and a nonlinear optimization algorithm. These algorithms emphasize the recovery of numerous optical parameters, including low-order wavefront composition described by Zernike polynomial terms and high-order wavefront described by a point-by-point map, location of instrument best focus, focal ratio, exit-pupil amplitude, the morphology of any extended object, and optical jitter. The secondary objective of this paper is to report on the relative accuracies of these algorithms for the ISIM instrument tests, and a comparison of their computational complexity and their performance on central and graphical processing unit clusters. From a phase-retrieval perspective, the ISIM test campaign includes a variety of source illumination bandwidths, various image-plane sampling criteria above and below the Nyquist- Shannon critical sampling value, various extended object sizes, and several other impactful effects.

Quantitative Microbial Risk Assessment Tutorial Installation of Software for Watershed Modeling in Support of QMRA - Updated 2017

EPA Science Inventory

This tutorial provides instructions for accessing, retrieving, and downloading the following software to install on a host computer in support of Quantitative Microbial Risk Assessment (QMRA) modeling: • QMRA Installation • SDMProjectBuilder (which includes the Microbial ...

ENVISAT Land Surface Processes. Phase 2

NASA Technical Reports Server (NTRS)

vandenHurk, B. J. J. M.; Su, Z.; Verhoef, W.; Menenti, M.; Li, Z.-L.; Wan, Z.; Moene, A. F.; Roerink, G.; Jia, I.

2002-01-01

This is a progress report of the 2nd phase of the project ENVISAT- Land Surface Processes, which has a 3-year scope. In this project, preparative research is carried out aiming at the retrieval of land surface characteristics from the ENVISAT sensors MERIS and AATSR, for assimilation into a system for Numerical Weather Prediction (NWP). Where in the 1st phase a number of first shot experiments were carried out (aiming at gaining experience with the retrievals and data assimilation procedures), the current 2nd phase has put more emphasis on the assessment and improvement of the quality of the retrieved products. The forthcoming phase will be devoted mainly to the data assimilation experiments and the assessment of the added value of the future ENVISAT products for NWP forecast skill. Referring to the retrieval of albedo, leaf area index and atmospheric corrections, preliminary radiative transfer calculations have been carried out that should enable the retrieval of these parameters once AATSR and MERIS data become available. However, much of this work is still to be carried out. An essential part of work in this area is the design and implementation of software that enables an efficient use of MODTRAN(sub 4) radiative transfer code, and during the current project phase familiarization with these new components has been achieved. Significant progress has been made with the retrieval of component temperatures from directional ATSR-images, and the calculation of surface turbulent heat fluxes from these data. The impact of vegetation cover on the retrieved component temperatures appears manageable, and preliminary comparison of foliage temperature to air temperatures were encouraging. The calculation of surface fluxes using the SEBI concept,which includes a detailed model of the surface roughness ratio, appeared to give results that were in reasonable agreement with local measurements with scintillometer devices. The specification of the atmospheric boundary conditions appears a crucial component, and the use of first guess estimates from the RACMO models partially explains the success. Earlier data assimilation experiments with directional surface temperatures have been analysed a bit further and were also compared to results obtained from directly modeling the surface roughness ratio. Results between these calculations and the data assimilation results appeared well comparable, but a full test in which the surface roughness model is allowed to play a free role during the data assimilation process has yet to be carried out. A considerable number of tasks that have yet to be carried out during Phase 3 has been formulated.

Altered object exploration but not temporal order memory retrieval in an object recognition test following treatment of rats with the group II metabotropic glutamate receptor agonist LY379268.

PubMed

Lins, Brittney R; Ballendine, Stephanie A; Howland, John G

2014-02-07

Temporal order memory refers to the ability to distinguish past experiences in the order that they occurred. Temporal order memory for objects is often tested in rodents using spontaneous object recognition paradigms. The circuitry mediating memory in these tests is distributed and involves ionotropic glutamate receptors in the perirhinal cortex and medial prefrontal cortex. It is unknown what role, if any, metabotropic glutamate receptors have in temporal order memory for objects. The present experiment examined the role of metabotropic glutamate receptors in temporal memory retrieval using the group II metabotropic glutamate receptor selective agonist LY379268. Rats were trained on a temporal memory test with three phases: two sample phases (60 min between them) in which rats explored two novel objects and a test phase (60 min after the second sample phase) which included a copy of each object previously encountered. Under these conditions, we confirmed that rats showed a significant exploratory preference for the object presented during the first sample phase. In a second experiment, we found that LY379268 (0.3, 1.0, or 3.0mg/kg; i.p.; 30 min before the test phase) had no effect on temporal memory retrieval but dose-dependently reduced time spent exploring the objects. Our results show that enhancing mGluR2 activity under conditions when TM is intact does not influence memory retrieval. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Characterization of the hollow fiber assay for the determination of microtubule disruption in vivo.

PubMed

Suggitt, Marie; Swaine, David J; Pettit, George R; Bibby, Michael C

2004-10-01

The hollow fiber assay is used successfully as a routine in vivo screening model to quantitatively define anticancer activity by the National Cancer Institute. This study investigates whether the hollow fiber assay can be used as a short-term in vivo model to demonstrate specific pharmacodynamic end points, namely microtubule and cell cycle disruption. The growth of A549 cells was characterized within hollow fibers over 5 days in vivo at both subcutaneous (s.c.) and intraperitoneal (i.p.) sites. Drugs were administered on day 4 (i.p.). At 24 hours, cells were retrieved from fibers at both i.p. and s.c. sites of paclitaxel-treated (20 mg/kg) and combretastatin A1 phosphate-treated (150 mg/kg) mice. Cell cycle analysis after paclitaxel treatment revealed a mean G(2)-M phase population of 48.04% (i.p.) and 25.76% (s.c.) compared with vehicle group mice (6.78 and 5.56%, respectively; P = <0.001 and 0.005, respectively). Tumor cells retrieved from combretastatin A1 phosphate-treated mice had a mean G2-M phase population of 36.3% (i.p.) and 29.36% (s.c.) compared with cells retrieved from vehicle group mice (5.58 and 5.49%, respectively; P = <0.001). Using fluorescence and laser-confocal microscopy, paclitaxel was revealed to induce the formation of spindle asters and tubulin polymerization. Combretastatin A1 phosphate was shown to hold cells in mitosis. Changes in nuclear morphology were also observed. These data demonstrate that the hollow fiber assay can be used as a short-term in vivo model for studying the pharmacodynamic effects of both standard and novel compounds on microtubules. Evidence has also been provided to support the routine use of the in vivo hollow fiber assay for demonstrating the mechanism of action of a drug.

A Retrieved Context Account of Spacing and Repetition Effects in Free Recall

ERIC Educational Resources Information Center

Siegel, Lynn L.; Kahana, Michael J.

2014-01-01

Repeating an item in a list benefits recall performance, and this benefit increases when the repetitions are spaced apart (Madigan, 1969; Melton, 1970). Retrieved context theory incorporates 2 mechanisms that account for these effects: contextual variability and study-phase retrieval. Specifically, if an item presented at position "i" is…

Redefining the lower statistical limit in x-ray phase-contrast imaging

NASA Astrophysics Data System (ADS)

Marschner, M.; Birnbacher, L.; Willner, M.; Chabior, M.; Fehringer, A.; Herzen, J.; Noël, P. B.; Pfeiffer, F.

2015-03-01

Phase-contrast x-ray computed tomography (PCCT) is currently investigated and developed as a potentially very interesting extension of conventional CT, because it promises to provide high soft-tissue contrast for weakly absorbing samples. For data acquisition several images at different grating positions are combined to obtain a phase-contrast projection. For short exposure times, which are necessary for lower radiation dose, the photon counts in a single stepping position are very low. In this case, the currently used phase-retrieval does not provide reliable results for some pixels. This uncertainty results in statistical phase wrapping, which leads to a higher standard deviation in the phase-contrast projections than theoretically expected. For even lower statistics, the phase retrieval breaks down completely and the phase information is lost. New measurement procedures rely on a linear approximation of the sinusoidal phase stepping curve around the zero crossings. In this case only two images are acquired to obtain the phase-contrast projection. The approximation is only valid for small phase values. However, typically nearly all pixels are within this regime due to the differential nature of the signal. We examine the statistical properties of a linear approximation method and illustrate by simulation and experiment that the lower statistical limit can be redefined using this method. That means that the phase signal can be retrieved even with very low photon counts and statistical phase wrapping can be avoided. This is an important step towards enhanced image quality in PCCT with very low photon counts.

The Operational MODIS Cloud Optical and Microphysical Property Product: Overview of the Collection 6 Algorithm and Preliminary Results

NASA Technical Reports Server (NTRS)

Platnick, Steven; King, Michael D.; Wind, Galina; Amarasinghe, Nandana; Marchant, Benjamin; Arnold, G. Thomas

2012-01-01

Operational Moderate Resolution Imaging Spectroradiometer (MODIS) retrievals of cloud optical and microphysical properties (part of the archived products MOD06 and MYD06, for MODIS Terra and Aqua, respectively) are currently being reprocessed along with other MODIS Atmosphere Team products. The latest "Collection 6" processing stream, which is expected to begin production by summer 2012, includes updates to the previous cloud retrieval algorithm along with new capabilities. The 1 km retrievals, based on well-known solar reflectance techniques, include cloud optical thickness, effective particle radius, and water path, as well as thermodynamic phase derived from a combination of solar and infrared tests. Being both global and of high spatial resolution requires an algorithm that is computationally efficient and can perform over all surface types. Collection 6 additions and enhancements include: (i) absolute effective particle radius retrievals derived separately from the 1.6 and 3.7 !-lm bands (instead of differences relative to the standard 2.1 !-lm retrieval), (ii) comprehensive look-up tables for cloud reflectance and emissivity (no asymptotic theory) with a wind-speed interpolated Cox-Munk BRDF for ocean surfaces, (iii) retrievals for both liquid water and ice phases for each pixel, and a subsequent determination of the phase based, in part, on effective radius retrieval outcomes for the two phases, (iv) new ice cloud radiative models using roughened particles with a specified habit, (v) updated spatially-complete global spectral surface albedo maps derived from MODIS Collection 5, (vi) enhanced pixel-level uncertainty calculations incorporating additional radiative error sources including the MODIS L1 B uncertainty index for assessing band and scene-dependent radiometric uncertainties, (v) and use of a new 1 km cloud top pressure/temperature algorithm (also part of MOD06) for atmospheric corrections and low cloud non-unity emissivity temperature adjustments.

Information content of ozone retrieval algorithms

NASA Technical Reports Server (NTRS)

Rodgers, C.; Bhartia, P. K.; Chu, W. P.; Curran, R.; Deluisi, J.; Gille, J. C.; Hudson, R.; Mateer, C.; Rusch, D.; Thomas, R. J.

1989-01-01

The algorithms are characterized that were used for production processing by the major suppliers of ozone data to show quantitatively: how the retrieved profile is related to the actual profile (This characterizes the altitude range and vertical resolution of the data); the nature of systematic errors in the retrieved profiles, including their vertical structure and relation to uncertain instrumental parameters; how trends in the real ozone are reflected in trends in the retrieved ozone profile; and how trends in other quantities (both instrumental and atmospheric) might appear as trends in the ozone profile. No serious deficiencies were found in the algorithms used in generating the major available ozone data sets. As the measurements are all indirect in someway, and the retrieved profiles have different characteristics, data from different instruments are not directly comparable.

Error analysis applied to several inversion techniques used for the retrieval of middle atmospheric constituents from limb-scanning MM-wave spectroscopic measurements

NASA Technical Reports Server (NTRS)

Puliafito, E.; Bevilacqua, R.; Olivero, J.; Degenhardt, W.

1992-01-01

The formal retrieval error analysis of Rodgers (1990) allows the quantitative determination of such retrieval properties as measurement error sensitivity, resolution, and inversion bias. This technique was applied to five numerical inversion techniques and two nonlinear iterative techniques used for the retrieval of middle atmospheric constituent concentrations from limb-scanning millimeter-wave spectroscopic measurements. It is found that the iterative methods have better vertical resolution, but are slightly more sensitive to measurement error than constrained matrix methods. The iterative methods converge to the exact solution, whereas two of the matrix methods under consideration have an explicit constraint, the sensitivity of the solution to the a priori profile. Tradeoffs of these retrieval characteristics are presented.

The national coal-resources data system of the U.S. geological survey

USGS Publications Warehouse

Carter, M.D.

1976-01-01

The National Coal Resources Data System (NCRDS) was designed by the U.S. Geological Survey (USGS) to meet the increasing demands for rapid retrieval of information on coal location, quantity, quality, and accessibility. An interactive conversational query system devised by the USGS retrieves information from the data bank through a standard computer terminal. The system is being developed in two phases. Phase I, which currently is available on a limited basis, contains published areal resource and chemical data. The primary objective of this phase is to retrieve, calculate, and tabulate coal-resource data by area on a local, regional, or national scale. Factors available for retrieval include: state, county, quadrangle, township, coal field, coal bed, formation, geologic age, source and reliability of data, and coal-bed rank, thickness, overburden, and tonnage, or any combinations of variables. In addition, the chemical data items include individual values for proximate and ultimate analyses, BTU value, and several other physical and chemical tests. Information will be validated and deleted or updated as needed. Phase II is being developed to store, retrieve, and manipulate basic point source coal data (e.g., field observations, drill-hole logs), including geodetic location; bed thickness; depth of burial; moisture; ash; sulfur; major-, minor-, and trace-element content; heat value; and characteristics of overburden, roof rocks, and floor rocks. The computer system may be used to generate interactively structure-contour or isoline maps of the physical and chemical characteristics of a coal bed or to calculate coal resources. ?? 1976.

Practicing more retrieval routes leads to greater memory retention.

PubMed

Zheng, Jun; Zhang, Wei; Li, Tongtong; Liu, Zhaomin; Luo, Liang

2016-09-01

A wealth of research has shown that retrieval practice plays a significant role in improving memory retention. The current study focused on one simple yet rarely examined question: would repeated retrieval using two different retrieval routes or using the same retrieval route twice lead to greater long-term memory retention? Participants elaborately learned 22 Japanese-Chinese translation word pairs using two different mediators. Half an hour after the initial study phase, the participants completed two retrieval sessions using either one mediator (Tm1Tm1) or two different mediators (Tm1Tm2). On the final test, which was performed 1week after the retrieval practice phase, the participants received only the cue with a request to report the mediator (M1 or M2) followed by the target (Experiment 1) or only the mediator (M1 or M2) with a request to report the target (Experiment 2). The results of Experiment 1 indicated that the participants who practiced under the Tm1Tm2 condition exhibited greater target retention than those who practiced under the Tm1Tm1 condition. This difference in performance was due to the significant disadvantage in mediator retrieval and decoding of the unpracticed mediator under the Tm1Tm1 condition. Although mediators were provided to participants on the final test in Experiment 2, decoding of the unpracticed mediators remained less effective than decoding of the practiced mediators. We conclude that practicing multiple retrieval routes leads to greater memory retention than focusing on a single retrieval route. Thus, increasing retrieval variability during repeated retrieval practice indeed significantly improves long-term retention in a delay test. Copyright © 2016 Elsevier B.V. All rights reserved.

Determination of the stacking fault density in highly defective single GaAs nanowires by means of coherent diffraction imaging

NASA Astrophysics Data System (ADS)

Davtyan, Arman; Biermanns, Andreas; Loffeld, Otmar; Pietsch, Ullrich

2016-06-01

Coherent x-ray diffraction imaging is used to measure diffraction patterns from individual highly defective nanowires, showing a complex speckle pattern instead of well-defined Bragg peaks. The approach is tested for nanowires of 500 nm diameter and 500 nm height predominately composed by zinc-blende (ZB) and twinned zinc-blende (TZB) phase domains. Phase retrieval is used to reconstruct the measured 2-dimensional intensity patterns recorded from single nanowires with 3.48 nm and 0.98 nm spatial resolution. Whereas the speckle amplitudes and distribution are perfectly reconstructed, no unique solution could be obtained for the phase structure. The number of phase switches is found to be proportional to the number of measured speckles and follows a narrow number distribution. Using data with 0.98 nm spatial resolution the mean number of phase switches is in reasonable agreement with estimates taken from TEM. However, since the resolved phase domain still is 3-4 times larger than a single GaAs bilayer we explain the non-ambiguous phase reconstruction by the fact that depending on starting phase and sequence of subroutines used during the phase retrieval the retrieved phase domain host a different sequence of randomly stacked bilayers. Modelling possible arrangements of bilayer sequences within a phase domain demonstrate that the complex speckle patterns measured can indeed be explained by the random arrangement of the ZB and TZB phase domains.

Retriever, a multiprotein complex for retromer-independent endosomal cargo recycling

PubMed Central

McNally, Kerrie E.; Faulkner, Rebecca; Steinberg, Florian; Gallon, Matthew; Ghai, Rajesh; Pim, David; Langton, Paul; Pearson, Neil; Danson, Chris M.; Nägele, Heike; Morris, Lindsey M; Singla, Arnika; Overlee, Brittany L; Heesom, Kate J.; Sessions, Richard; Banks, Lawrence; Collins, Brett M; Berger, Imre; Billadeau, Daniel D.; Burstein, Ezra; Cullen, Peter J.

2018-01-01

Following endocytosis and entry into the endosomal network, integral membrane proteins undergo sorting for lysosomal degradation or are alternatively retrieved and recycled back to the cell surface. Here we describe the discovery of an ancient and conserved multi-protein complex which orchestrates cargo retrieval and recycling and importantly, is biochemically and functionally distinct to the established retromer pathway. Composed of a heterotrimer of DSCR3, C16orf62 and VPS29, and bearing striking similarity with retromer, we have called this complex ‘retriever’. We establish that retriever associates with the cargo adaptor sorting nexin 17 (SNX17) and couples to the CCC and WASH complexes to prevent lysosomal degradation and promote cell surface recycling of α5β1-integrin. Through quantitative proteomic analysis we identify over 120 cell surface proteins, including numerous integrins, signalling receptors and solute transporters, which require SNX17-retriever to maintain their surface levels. Our identification of retriever establishes a major new endosomal retrieval and recycling pathway. PMID:28892079

Measurement Matrix Design for Phase Retrieval Based on Mutual Information

NASA Astrophysics Data System (ADS)

Shlezinger, Nir; Dabora, Ron; Eldar, Yonina C.

2018-01-01

In phase retrieval problems, a signal of interest (SOI) is reconstructed based on the magnitude of a linear transformation of the SOI observed with additive noise. The linear transform is typically referred to as a measurement matrix. Many works on phase retrieval assume that the measurement matrix is a random Gaussian matrix, which, in the noiseless scenario with sufficiently many measurements, guarantees invertability of the transformation between the SOI and the observations, up to an inherent phase ambiguity. However, in many practical applications, the measurement matrix corresponds to an underlying physical setup, and is therefore deterministic, possibly with structural constraints. In this work we study the design of deterministic measurement matrices, based on maximizing the mutual information between the SOI and the observations. We characterize necessary conditions for the optimality of a measurement matrix, and analytically obtain the optimal matrix in the low signal-to-noise ratio regime. Practical methods for designing general measurement matrices and masked Fourier measurements are proposed. Simulation tests demonstrate the performance gain achieved by the proposed techniques compared to random Gaussian measurements for various phase recovery algorithms.

Shape Measurement by Means of Phase Retrieval using a Spatial Light Modulator

NASA Astrophysics Data System (ADS)

Agour, Mostafa; Huke, Philipp; Kopylow, Christoph V.; Falldorf, Claas

2010-04-01

We present a novel approach to investigate the shape of a diffusely reflecting technical object. It is based on a combination of a multiple-illumination contouring procedure and phase retrieval from a set of intensity measurements. Special consideration is given to the design of the experimental configuration for phase retrieval and the iterative algorithm to extract the 3D phase map. It is mainly based on a phase-only spatial light modulator (SLM) in the Fourier domain of a 4f-imaging system. The SLM is used to modulate the light incident in the Fourier plane with the transfer function of propagation. Thus, a set of consecutive intensity measurements of the wave field scattered by the investigated object in various propagation states can be realized in a common recording plane. In contrast to already existing methods, no mechanical adjustment is required during the recording process and thus the measuring time is considerably reduced. The method is applied to investigate the shape of micro-objects obtained from a metalforming process. Finally, the experimental results are compared to those provided by a standard interferometric contouring procedure.

Retrieval of Ice Cloud Properties Using Variable Phase Functions

NASA Astrophysics Data System (ADS)

Heck, Patrick W.; Minnis, Patrick; Yang, Ping; Chang, Fu-Lung; Palikonda, Rabindra; Arduini, Robert F.; Sun-Mack, Sunny

2009-03-01

An enhancement to NASA Langley's Visible Infrared Solar-infrared Split-window Technique (VISST) is developed to identify and account for situations when errors are induced by using smooth ice crystals. The retrieval scheme incorporates new ice cloud phase functions that utilize hexagonal crystals with roughened surfaces. In some situations, cloud optical depths are reduced, hence, cloud height is increased. Cloud effective particle size also changes with the roughened ice crystal models which results in varied effects on the calculation of ice water path. Once validated and expanded, the new approach will be integrated in the CERES MODIS algorithm and real-time retrievals at Langley.

Investigations on the change of texture of plant cells due to preservative treatments by digital holographic microscopy

NASA Astrophysics Data System (ADS)

Vora, Priyanka; Anand, Arun

2014-10-01

Texture change is observed in preserved fruits and vegetables. Responsible factors for texture change during preservative treatments are cell morphology, cell wall structure, cell turger, water content and some biochemical components, and also the environmental conditions. Digital Holographic microscopy (DHM) is a quantitative phase contrast imaging technique, which provides three dimensional optical thickness profiles of transparent specimen. Using DHM the morphology of plant cells preserved by refrigeration or stored in vinegar or in sodium chloride can be obtained. This information about the spatio-temporal evolution of optical volume and thickness can be an important tool in area of food processing. Also from the three dimensional images, the texture of the cell can be retrieved and can be investigated under varying conditions.

Phase transitions in restricted Boltzmann machines with generic priors

NASA Astrophysics Data System (ADS)

Barra, Adriano; Genovese, Giuseppe; Sollich, Peter; Tantari, Daniele

2017-10-01

We study generalized restricted Boltzmann machines with generic priors for units and weights, interpolating between Boolean and Gaussian variables. We present a complete analysis of the replica symmetric phase diagram of these systems, which can be regarded as generalized Hopfield models. We underline the role of the retrieval phase for both inference and learning processes and we show that retrieval is robust for a large class of weight and unit priors, beyond the standard Hopfield scenario. Furthermore, we show how the paramagnetic phase boundary is directly related to the optimal size of the training set necessary for good generalization in a teacher-student scenario of unsupervised learning.

Implicit proactive interference, age, and automatic versus controlled retrieval strategies.

PubMed

Ikier, Simay; Yang, Lixia; Hasher, Lynn

2008-05-01

We assessed the extent to which implicit proactive interference results from automatic versus controlled retrieval among younger and older adults. During a study phase, targets (e.g., "ALLERGY") either were or were not preceded by nontarget competitors (e.g., "ANALOGY"). After a filled interval, the participants were asked to complete word fragments, some of which cued studied words (e.g., "A_L_ _GY"). Retrieval strategies were identified by the difference in response speed between a phase containing fragments that cued only new words and a phase that included a mix of fragments cuing old and new words. Previous results were replicated: Proactive interference was found in implicit memory, and the negative effects were greater for older than for younger adults. Novel findings demonstrate two retrieval processes that contribute to interference: an automatic one that is age invariant and a controlled process that can reduce the magnitude of the automatic interference effects. The controlled process, however, is used effectively only by younger adults. This pattern of findings potentially explains age differences in susceptibility to proactive interference.

Phase retrieval in digital speckle pattern interferometry by application of two-dimensional active contours called snakes.

PubMed

Federico, Alejandro; Kaufmann, Guillermo H

2006-03-20

We propose a novel approach to retrieving the phase map coded by a single closed-fringe pattern in digital speckle pattern interferometry, which is based on the estimation of the local sign of the quadrature component. We obtain the estimate by calculating the local orientation of the fringes that have previously been denoised by a weighted smoothing spline method. We carry out the procedure of sign estimation by determining the local abrupt jumps of size pi in the orientation field of the fringes and by segmenting the regions defined by these jumps. The segmentation method is based on the application of two-dimensional active contours (snakes), with which one can also estimate absent jumps, i.e., those that cannot be detected from the local orientation of the fringes. The performance of the proposed phase-retrieval technique is evaluated for synthetic and experimental fringes and compared with the results obtained with the spiral-phase- and Fourier-transform methods.

Using Radar, Lidar, and Radiometer measurements to Classify Cloud Type and Study Middle-Level Cloud Properties

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Zhien

2010-06-29

The project is mainly focused on the characterization of cloud macrophysical and microphysical properties, especially for mixed-phased clouds and middle level ice clouds by combining radar, lidar, and radiometer measurements available from the ACRF sites. First, an advanced mixed-phase cloud retrieval algorithm will be developed to cover all mixed-phase clouds observed at the ACRF NSA site. The algorithm will be applied to the ACRF NSA observations to generate a long-term arctic mixed-phase cloud product for model validations and arctic mixed-phase cloud processes studies. To improve the representation of arctic mixed-phase clouds in GCMs, an advanced understanding of mixed-phase cloud processesmore » is needed. By combining retrieved mixed-phase cloud microphysical properties with in situ data and large-scale meteorological data, the project aim to better understand the generations of ice crystals in supercooled water clouds, the maintenance mechanisms of the arctic mixed-phase clouds, and their connections with large-scale dynamics. The project will try to develop a new retrieval algorithm to study more complex mixed-phase clouds observed at the ACRF SGP site. Compared with optically thin ice clouds, optically thick middle level ice clouds are less studied because of limited available tools. The project will develop a new two wavelength radar technique for optically thick ice cloud study at SGP site by combining the MMCR with the W-band radar measurements. With this new algorithm, the SGP site will have a better capability to study all ice clouds. Another area of the proposal is to generate long-term cloud type classification product for the multiple ACRF sites. The cloud type classification product will not only facilitates the generation of the integrated cloud product by applying different retrieval algorithms to different types of clouds operationally, but will also support other research to better understand cloud properties and to validate model simulations. The ultimate goal is to improve our cloud classification algorithm into a VAP.« less

A novel methodology for querying web images

NASA Astrophysics Data System (ADS)

Prabhakara, Rashmi; Lee, Ching Cheng

2005-01-01

Ever since the advent of Internet, there has been an immense growth in the amount of image data that is available on the World Wide Web. With such a magnitude of image availability, an efficient and effective image retrieval system is required to make use of this information. This research presents an effective image matching and indexing technique that improvises on existing integrated image retrieval methods. The proposed technique follows a two-phase approach, integrating query by topic and query by example specification methods. The first phase consists of topic-based image retrieval using an improved text information retrieval (IR) technique that makes use of the structured format of HTML documents. It consists of a focused crawler that not only provides for the user to enter the keyword for the topic-based search but also, the scope in which the user wants to find the images. The second phase uses the query by example specification to perform a low-level content-based image match for the retrieval of smaller and relatively closer results of the example image. Information related to the image feature is automatically extracted from the query image by the image processing system. A technique that is not computationally intensive based on color feature is used to perform content-based matching of images. The main goal is to develop a functional image search and indexing system and to demonstrate that better retrieval results can be achieved with this proposed hybrid search technique.

A novel methodology for querying web images

NASA Astrophysics Data System (ADS)

Prabhakara, Rashmi; Lee, Ching Cheng

2004-12-01

Ever since the advent of Internet, there has been an immense growth in the amount of image data that is available on the World Wide Web. With such a magnitude of image availability, an efficient and effective image retrieval system is required to make use of this information. This research presents an effective image matching and indexing technique that improvises on existing integrated image retrieval methods. The proposed technique follows a two-phase approach, integrating query by topic and query by example specification methods. The first phase consists of topic-based image retrieval using an improved text information retrieval (IR) technique that makes use of the structured format of HTML documents. It consists of a focused crawler that not only provides for the user to enter the keyword for the topic-based search but also, the scope in which the user wants to find the images. The second phase uses the query by example specification to perform a low-level content-based image match for the retrieval of smaller and relatively closer results of the example image. Information related to the image feature is automatically extracted from the query image by the image processing system. A technique that is not computationally intensive based on color feature is used to perform content-based matching of images. The main goal is to develop a functional image search and indexing system and to demonstrate that better retrieval results can be achieved with this proposed hybrid search technique.

How Often and Why MODIS Cloud Property Retrievals Fail for Liquid-Phase Clouds over Ocean? a Comprehensive Analysis Based on a-Train Observations

NASA Astrophysics Data System (ADS)

Zhang, Z.; Cho, H. M.; Platnick, S. E.; Meyer, K.; Lebsock, M. D.

2014-12-01

The cloud optical thickness (τ) and droplet effective radius (re) are two key cloud parameters retrieved by MODIS (Moderate Resolution Imaging Spectroradiometer). These MODIS cloud products are widely used in a broad range of earth system science applications. In this paper, we present a comprehensive analysis of the failed cloud τ and/or re retrievals for liquid-phase clouds over ocean in the Collection 6 MODIS cloud product. The main findings from this study are summarized as follows: MODIS retrieval failure rates for marine boundary layer (MBL) clouds have a strong dependence on the spectral combination used for retrieval (e.g., 0.86 + 2.1 µm vs. 0.8 + 3.7 µm) and the cloud morphology (i.e., "good" pixels vs. partly cloudy (PCL) pixels). Combining all clear-sky-restoral (CSR) categories (CSR=0,1 and 3), the 0.86 + 2.1 µm and 0.86 + 3.7 µm spectral combinations have an overall failure rate of about 20% and 12%, respectively (See figure below). The PCL pixels (CSR=1 & 3) have significantly higher failure rates and contribute more to the total failure population than the "good" (CSR=0) pixels. The majority of the failed retrievals are caused by the re too large failure, which explains about 85% and 70% of the failed 0.86 + 2.1 µm and 0.86 + 3.7 µm retrievals, respectively. The remaining failures are either due to the re too small failure or τ retrieval failure. The geographical distribution of failure rates has a significant dependence on cloud regime, lower over the coastal stratocumulus cloud regime and higher over the broken trade-wind cumulus cloud regime over open oceans. Enhanced retrieval failure rates are found when MBL clouds have high sub-pixel inhomogeneity , or are located at special Sun-satellite viewing geometries, such as sunglint, large viewing or solar zenith angle, or cloudbow and glory angles, or subject to cloud masking, cloud overlapping and/or cloud phase retrieval issues. About 80% of the failure retrievals can be attributed to at least one or more potential reasons mentioned above. Collocated radar reflectivity observations from CloudSat suggest that the remaining 20% are unlikely to be retrieval artifacts, but reflection of true cloud microphysics, i.e., the true is either truly very small or very large.

Time-dependent phase shift of a retrieved pulse in off-resonant electromagnetically-induced-transparency-based light storage

NASA Astrophysics Data System (ADS)

Maynard, M.-A.; Bouchez, R.; Lugani, J.; Bretenaker, F.; Goldfarb, F.; Brion, E.

2015-11-01

We report measurements of the time-dependent phases of the leak and retrieved pulses obtained in electromagnetically-induced-transparency storage experiments with metastable helium vapor at room temperature. In particular, we investigate the influence of the optical detuning at two-photon resonance and provide numerical simulations of the full dynamical Maxwell-Bloch equations, which allow us to account for the experimental results.

High Resolution Imaging Using Phase Retrieval. Volume 2

DTIC Science & Technology

1991-10-01

aberrations of the telescope. It will also correct aberrations due to atmospheric turbulence for a ground- based telescope, and can be used with several other...retrieval algorithm, based on the Ayers/Dainty blind deconvolution algorithm, was also developed. A new methodology for exploring the uniqueness of phase...Simulation Experiments ..................... 42 3.3.1 Initial Simulations with Noisy Modulus Data ..... 45 3.3.2 Simulations of a Space- Based Amplitude

Ground-based remote sensing of thin clouds in the Arctic

NASA Astrophysics Data System (ADS)

Garrett, T. J.; Zhao, C.

2013-05-01

This paper describes a method for using interferometer measurements of downwelling thermal radiation to retrieve the properties of single-layer clouds. Cloud phase is determined from ratios of thermal emission in three "micro-windows" at 862.5 cm-1, 935.8 cm-1, and 988.4 cm-1 where absorption by water vapour is particularly small. Cloud microphysical and optical properties are retrieved from thermal emission in the first two of these micro-windows, constrained by the transmission through clouds of primarily stratospheric ozone emission at 1040 cm-1. Assuming a cloud does not approximate a blackbody, the estimated 95% confidence retrieval errors in effective radius re, visible optical depth τ, number concentration N, and water path WP are, respectively, 10%, 20%, 38% (55% for ice crystals), and 16%. Applied to data from the Atmospheric Radiation Measurement programme (ARM) North Slope of Alaska - Adjacent Arctic Ocean (NSA-AAO) site near Barrow, Alaska, retrievals show general agreement with both ground-based microwave radiometer measurements of liquid water path and a method that uses combined shortwave and microwave measurements to retrieve re, τ and N. Compared to other retrieval methods, advantages of this technique include its ability to characterise thin clouds year round, that water vapour is not a primary source of retrieval error, and that the retrievals of microphysical properties are only weakly sensitive to retrieved cloud phase. The primary limitation is the inapplicability to thicker clouds that radiate as blackbodies and that it relies on a fairly comprehensive suite of ground based measurements.

Influence of controlled encoding and retrieval facilitation on memory performance in patients with different profiles of mild cognitive impairment.

PubMed

Perri, Roberta; Monaco, Marco; Fadda, Lucia; Serra, Laura; Marra, Camillo; Caltagirone, Carlo; Bruni, Amalia C; Curcio, Sabrina; Bozzali, M; Carlesimo, Giovanni A

2015-01-01

Memory tests able to differentiate encoding and retrieval processes from the memoranda storing ones should be used to differentiate patients in a very early phase of AD. In fact, individuals with mild cognitive impairment (MCI) can be characterized by two different memory profiles: a pure amnestic one (with poor learning and retrieval and poor improvement when encoding is assisted and retrieval is facilitated) and a dysexecutive one (with inefficient encoding and/or poor retrieval strategies and improvement with assisted encoding and retrieval). The amnestic profile characterizes subjects affected by medio-temporal atrophy typical of AD. In this study, a Grober-Buschke memory procedure was used to evaluate normal controls and MCI patients with different cognitive profiles: pure amnestic (aMCIsd), amnestic plus other cognitive impairments (aMCImd) and non-amnestic (naMCI). An index of sensitivity of cueing (ISC) measured the advantage passing from free to cued recall. Results showed that both strategic and consolidation abilities were impaired in the aMCIsd and aMCImd groups and were preserved in the naMCI group. aMCImd, however, compensated the memory deficit with assisted encoding and retrieval, but aMCIsd performed very poorly. When MCI subjects were defined according to the ISC value, subjects with poor ISC were primarily in the aMCIsd group and, to a lesser extent, in the aMCImd group and the naMCI group. Finally, patients with a poor ISC showed cerebral atrophy documented in the precocious phase of AD and the retrosplenial cerebral areas seemed to be the most useful areas for identifying patients in the early phase of AD.

Numerical phase retrieval from beam intensity measurements in three planes

NASA Astrophysics Data System (ADS)

Bruel, Laurent

2003-05-01

A system and method have been developed at CEA to retrieve phase information from multiple intensity measurements along a laser beam. The device has been patented. Commonly used devices for beam measurement provide phase and intensity information separately or with a rather poor resolution whereas the MIROMA method provides both at the same time, allowing direct use of the results in numerical models. Usual phase retrieval algorithms use two intensity measurements, typically the image plane and the focal plane (Gerschberg-Saxton algorithm) related by a Fourier transform, or the image plane and a lightly defocus plane (D.L. Misell). The principal drawback of such iterative algorithms is their inability to provide unambiguous convergence in all situations. The algorithms can stagnate on bad solutions and the error between measured and calculated intensities remains unacceptable. If three planes rather than two are used, the data redundancy created confers to the method good convergence capability and noise immunity. It provides an excellent agreement between intensity determined from the retrieved phase data set in the image plane and intensity measurements in any diffraction plane. The method employed for MIROMA is inspired from GS algorithm, replacing Fourier transforms by a beam-propagating kernel with gradient search accelerating techniques and special care for phase branch cuts. A fast one dimensional algorithm provides an initial guess for the iterative algorithm. Applications of the algorithm on synthetic data find out the best reconstruction planes that have to be chosen. Robustness and sensibility are evaluated. Results on collimated and distorted laser beams are presented.

Phase accumulation tracking algorithm for effective index retrieval of fishnet metamaterials and other resonant guided wave networks

NASA Astrophysics Data System (ADS)

Feigenbaum, Eyal; Hiszpanski, Anna M.

2017-07-01

A phase accumulation tracking (PAT) algorithm is proposed and demonstrated for the retrieval of the effective index of fishnet metamaterials (FMMs) in order to avoid the multi-branch uncertainty problem. This algorithm tracks the phase and amplitude of the dominant propagation mode across the FMM slab. The suggested PAT algorithm applies to resonant guided wave networks having only one mode that carries the light between the two slab ends, where the FMM is one example of this metamaterials sub-class. The effective index is a net effect of positive and negative accumulated phase in the alternating FMM metal and dielectric layers, with a negative effective index occurring when negative phase accumulation dominates.

Application of phase-diverse phase retrieval to wavefront sensing in non-connected complicated pupil optics

NASA Astrophysics Data System (ADS)

Mao, Heng; Wang, Xiao; Zhao, Dazun

2007-07-01

Baseline algorithm, as a tool in wavefront sensing (WFS), incorporates the phase-diverse phase retrieval (PDPR) method with hybrid-unwrapping approach to ensure a unique pupil phase estimate with high WFS accuracy even in the case of high dynamic range aberration, as long as the pupil shape is of a convex set. However, for a complicated pupil, such as that in obstructed pupil optics, the said unwrapping approach would fail owing to the fake values at points located in obstructed areas of the pupil. Thus a modified unwrapping approach that can minimize the negative effects of the obstructed areas is proposed. Simulations have shown the validity of this unwrapping approach when it is embedded in Baseline algorithm.

Nucleus incertus inactivation impairs spatial learning and memory in rats.

PubMed

Nategh, Mohsen; Nikseresht, Sara; Khodagholi, Fariba; Motamedi, Fereshteh

2015-02-01

Nucleus incertus (NI) is a pontine nucleus which releases mainly GABA and relaxin-3 in rats. Its suggested functions include response to stress, arousal, and modulation of hippocampal theta rhythm. Since the role of NI in learning and memory has not been well characterized, therefore the involvement of this nucleus in spatial learning and memory and the aftermath hippocampal levels of c-fos and pCREB were evaluated. NI was targeted by implanting cannula in male rats. For reference memory, NI was inactivated by lidocaine (0.4 μl, 4%) at three stages of acquisition, consolidation and retrieval in Morris water maze paradigm. For working memory, NI was inactivated in acquisition and retrieval phases. Injection of lidocaine prior to the first training session of reference memory significantly increased the distance moved, suggesting that inactivation of NI delays acquisition in this spatial task. Inactivation also interfered with the retrieval phase of spatial reference memory, as the time in target quadrant for lidocaine group was less, and the escape latency was higher compared to the control group. However, no difference was observed in the consolidation phase. In the working memory task, with inter-trial intervals of 75 min, the escape latency was higher when NI was inactivated in the retrieval phase. In addition, c-fos and pCREB/CREB levels decreased in NI-inhibited rats. This study suggests that nucleus incertus might participate in acquisition of spatial reference, and retrieval of both spatial reference and working memory. Further studies should investigate possible roles of NI in the hippocampal plasticity. Copyright © 2014 Elsevier Inc. All rights reserved.

System and method for phase retrieval for radio telescope and antenna control

NASA Technical Reports Server (NTRS)

Dean, Bruce H. (Inventor)

2013-01-01

Disclosed herein are systems, methods, and non-transitory computer-readable storage media for radio phase retrieval. A system practicing the method gathers first data from radio waves associated with an object observed via a first aperture, gathers second data from radio waves associated with the object observed via an introduced second aperture associated with the first aperture, generates reduced noise data by incoherently subtracting the second data from the first data, and performs phase retrieval for the radio waves by modeling the reduced noise data using a single Fourier transform. The first and second apertures are at different positions, such as side by side. This approach can include determining a value Q which represents a ratio of wavelength times a focal ratio divided by pixel spacing. This information can be used to accurately measure and correct alignment errors or other optical system flaws in the apertures.

Three-dimensional imaging using phase retrieval with two focus planes

NASA Astrophysics Data System (ADS)

Ilovitsh, Tali; Ilovitsh, Asaf; Weiss, Aryeh; Meir, Rinat; Zalevsky, Zeev

2016-03-01

This work presents a technique for a full 3D imaging of biological samples tagged with gold-nanoparticles (GNPs) using only two images, rather than many images per volume as is currently needed for 3D optical sectioning microscopy. The proposed approach is based on the Gerchberg-Saxton (GS) phase retrieval algorithm. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. In addition, since the method requires the capturing of two images only, it can be suitable for 3D live cell imaging. The proposed concept is presented and validated both on simulated data as well as experimentally.

Image encryption using fingerprint as key based on phase retrieval algorithm and public key cryptography

NASA Astrophysics Data System (ADS)

Zhao, Tieyu; Ran, Qiwen; Yuan, Lin; Chi, Yingying; Ma, Jing

2015-09-01

In this paper, a novel image encryption system with fingerprint used as a secret key is proposed based on the phase retrieval algorithm and RSA public key algorithm. In the system, the encryption keys include the fingerprint and the public key of RSA algorithm, while the decryption keys are the fingerprint and the private key of RSA algorithm. If the users share the fingerprint, then the system will meet the basic agreement of asymmetric cryptography. The system is also applicable for the information authentication. The fingerprint as secret key is used in both the encryption and decryption processes so that the receiver can identify the authenticity of the ciphertext by using the fingerprint in decryption process. Finally, the simulation results show the validity of the encryption scheme and the high robustness against attacks based on the phase retrieval technique.

Fourier phase retrieval with a single mask by Douglas-Rachford algorithms.

PubMed

Chen, Pengwen; Fannjiang, Albert

2018-05-01

The Fourier-domain Douglas-Rachford (FDR) algorithm is analyzed for phase retrieval with a single random mask. Since the uniqueness of phase retrieval solution requires more than a single oversampled coded diffraction pattern, the extra information is imposed in either of the following forms: 1) the sector condition on the object; 2) another oversampled diffraction pattern, coded or uncoded. For both settings, the uniqueness of projected fixed point is proved and for setting 2) the local, geometric convergence is derived with a rate given by a spectral gap condition. Numerical experiments demonstrate global, power-law convergence of FDR from arbitrary initialization for both settings as well as for 3 or more coded diffraction patterns without oversampling. In practice, the geometric convergence can be recovered from the power-law regime by a simple projection trick, resulting in highly accurate reconstruction from generic initialization.

Three dimensional single molecule localization using a phase retrieved pupilfunction

PubMed Central

Liu, Sheng; Kromann, Emil B.; Krueger, Wesley D.; Bewersdorf, Joerg; Lidke, Keith A.

2013-01-01

Localization-based superresolution imaging is dependent on finding the positions of individualfluorophores in a sample by fitting the observed single-molecule intensity pattern to the microscopepoint spread function (PSF). For three-dimensional imaging, system-specific aberrations of theoptical system can lead to inaccurate localizations when the PSF model does not account for theseaberrations. Here we describe the use of phase-retrieved pupil functions to generate a more accuratePSF and therefore more accurate 3D localizations. The complex-valued pupil function containsinformation about the system-specific aberrations and can thus be used to generate the PSF forarbitrary defocus. Further, it can be modified to include depth dependent aberrations. We describethe phase retrieval process, the method for including depth dependent aberrations, and a fastfitting algorithm using graphics processing units. The superior localization accuracy of the pupilfunction generated PSF is demonstrated with dual focal plane 3D superresolution imaging ofbiological structures. PMID:24514501

Columnar aerosol properties over oceans by combining surface and aircraft measurements: sensitivity analysis.

PubMed

Zhang, T; Gordon, H R

1997-04-20

We report a sensitivity analysis for the algorithm presented by Gordon and Zhang [Appl. Opt. 34, 5552 (1995)] for inverting the radiance exiting the top and bottom of the atmosphere to yield the aerosol-scattering phase function [P(?)] and single-scattering albedo (omega(0)). The study of the algorithm's sensitivity to radiometric calibration errors, mean-zero instrument noise, sea-surface roughness, the curvature of the Earth's atmosphere, the polarization of the light field, and incorrect assumptions regarding the vertical structure of the atmosphere, indicates that the retrieved omega(0) has excellent stability even for very large values (~2) of the aerosol optical thickness; however, the error in the retrieved P(?) strongly depends on the measurement error and on the assumptions made in the retrieval algorithm. The retrieved phase functions in the blue are usually poor compared with those in the near infrared.

Leaf cuticle topography retrieved by using fringe projection

NASA Astrophysics Data System (ADS)

Martínez, Amalia; Rayas, J. A.; Cordero, Raúl R.; Balieiro, Daniela; Labbe, Fernando

2012-02-01

The combination (often referred to as phase-stepping profilometry, PSP) of the fringe projection technique and the phase-stepping method allowed us to retrieve topographic maps of cuticles isolated from the abaxial surface of leaves; these were in turn sampled from an apple tree ( Malus domestica) of the variety Golden Delicious. The topographic maps enabled us to assess the natural features on the illuminated surface and also to detect the whole-field spatial variations in the thickness of the cuticle. Most of our attention was paid to retrieve the highly-resolved elevation information from the cuticle surface, which included the trace (in the order of tens of micrometers) left by ribs and veins. We expect that the PSP application for retrieving the cuticle topography will facilitate further studies on the dispersion and coverage of state-of-the-art agrochemical compounds meant to improve the defending properties of the cuticle. Methodological details are provided below.

Robust phase retrieval of complex-valued object in phase modulation by hybrid Wirtinger flow method

NASA Astrophysics Data System (ADS)

Wei, Zhun; Chen, Wen; Yin, Tiantian; Chen, Xudong

2017-09-01

This paper presents a robust iterative algorithm, known as hybrid Wirtinger flow (HWF), for phase retrieval (PR) of complex objects from noisy diffraction intensities. Numerical simulations indicate that the HWF method consistently outperforms conventional PR methods in terms of both accuracy and convergence rate in multiple phase modulations. The proposed algorithm is also more robust to low oversampling ratios, loose constraints, and noisy environments. Furthermore, compared with traditional Wirtinger flow, sample complexity is largely reduced. It is expected that the proposed HWF method will find applications in the rapidly growing coherent diffractive imaging field for high-quality image reconstruction with multiple modulations, as well as other disciplines where PR is needed.

Noise robustness of a combined phase retrieval and reconstruction method for phase-contrast tomography.

PubMed

Kongskov, Rasmus Dalgas; Jørgensen, Jakob Sauer; Poulsen, Henning Friis; Hansen, Per Christian

2016-04-01

Classical reconstruction methods for phase-contrast tomography consist of two stages: phase retrieval and tomographic reconstruction. A novel algebraic method combining the two was suggested by Kostenko et al. [Opt. Express21, 12185 (2013)OPEXFF1094-408710.1364/OE.21.012185], and preliminary results demonstrated improved reconstruction compared with a given two-stage method. Using simulated free-space propagation experiments with a single sample-detector distance, we thoroughly compare the novel method with the two-stage method to address limitations of the preliminary results. We demonstrate that the novel method is substantially more robust toward noise; our simulations point to a possible reduction in counting times by an order of magnitude.

Ultra-high resolution coded wavefront sensor.

PubMed

Wang, Congli; Dun, Xiong; Fu, Qiang; Heidrich, Wolfgang

2017-06-12

Wavefront sensors and more general phase retrieval methods have recently attracted a lot of attention in a host of application domains, ranging from astronomy to scientific imaging and microscopy. In this paper, we introduce a new class of sensor, the Coded Wavefront Sensor, which provides high spatio-temporal resolution using a simple masked sensor under white light illumination. Specifically, we demonstrate megapixel spatial resolution and phase accuracy better than 0.1 wavelengths at reconstruction rates of 50 Hz or more, thus opening up many new applications from high-resolution adaptive optics to real-time phase retrieval in microscopy.

Fast angular synchronization for phase retrieval via incomplete information

NASA Astrophysics Data System (ADS)

Viswanathan, Aditya; Iwen, Mark

2015-08-01

We consider the problem of recovering the phase of an unknown vector, x ∈ ℂd, given (normalized) phase difference measurements of the form xjxk*/|xjxk*|, j,k ∈ {1,...,d}, and where xj* denotes the complex conjugate of xj. This problem is sometimes referred to as the angular synchronization problem. This paper analyzes a linear-time-in-d eigenvector-based angular synchronization algorithm and studies its theoretical and numerical performance when applied to a particular class of highly incomplete and possibly noisy phase difference measurements. Theoretical results are provided for perfect (noiseless) measurements, while numerical simulations demonstrate the robustness of the method to measurement noise. Finally, we show that this angular synchronization problem and the specific form of incomplete phase difference measurements considered arise in the phase retrieval problem - where we recover an unknown complex vector from phaseless (or magnitude) measurements.

Computational wavelength resolution for in-line lensless holography: phase-coded diffraction patterns and wavefront group-sparsity

NASA Astrophysics Data System (ADS)

Katkovnik, Vladimir; Shevkunov, Igor; Petrov, Nikolay V.; Egiazarian, Karen

2017-06-01

In-line lensless holography is considered with a random phase modulation at the object plane. The forward wavefront propagation is modelled using the Fourier transform with the angular spectrum transfer function. The multiple intensities (holograms) recorded by the sensor are random due to the random phase modulation and noisy with Poissonian noise distribution. It is shown by computational experiments that high-accuracy reconstructions can be achieved with resolution going up to the two thirds of the wavelength. With respect to the sensor pixel size it is a super-resolution with a factor of 32. The algorithm designed for optimal superresolution phase/amplitude reconstruction from Poissonian data is based on the general methodology developed for phase retrieval with a pixel-wise resolution in V. Katkovnik, "Phase retrieval from noisy data based on sparse approximation of object phase and amplitude", http://www.cs.tut.fi/ lasip/DDT/index3.html.

Color image encryption by using Yang-Gu mixture amplitude-phase retrieval algorithm in gyrator transform domain and two-dimensional Sine logistic modulation map

NASA Astrophysics Data System (ADS)

Sui, Liansheng; Liu, Benqing; Wang, Qiang; Li, Ye; Liang, Junli

2015-12-01

A color image encryption scheme is proposed based on Yang-Gu mixture amplitude-phase retrieval algorithm and two-coupled logistic map in gyrator transform domain. First, the color plaintext image is decomposed into red, green and blue components, which are scrambled individually by three random sequences generated by using the two-dimensional Sine logistic modulation map. Second, each scrambled component is encrypted into a real-valued function with stationary white noise distribution in the iterative amplitude-phase retrieval process in the gyrator transform domain, and then three obtained functions are considered as red, green and blue channels to form the color ciphertext image. Obviously, the ciphertext image is real-valued function and more convenient for storing and transmitting. In the encryption and decryption processes, the chaotic random phase mask generated based on logistic map is employed as the phase key, which means that only the initial values are used as private key and the cryptosystem has high convenience on key management. Meanwhile, the security of the cryptosystem is enhanced greatly because of high sensitivity of the private keys. Simulation results are presented to prove the security and robustness of the proposed scheme.

Involvement of hippocampal NMDA receptors in encoding and consolidation, but not retrieval, processes of spontaneous object location memory in rats.

PubMed

Yamada, Kazuo; Arai, Misaki; Suenaga, Toshiko; Ichitani, Yukio

2017-07-28

The hippocampus is thought to be involved in object location recognition memory, yet the contribution of hippocampal NMDA receptors to the memory processes, such as encoding, retention and retrieval, is unknown. First, we confirmed that hippocampal infusion of a competitive NMDA receptor antagonist, AP5 (2-amino-5-phosphonopentanoic acid, 20-40nmol), impaired performance of spontaneous object location recognition test but not that of novel object recognition test in Wistar rats. Next, the effects of hippocampal AP5 treatment on each process of object location recognition memory were examined with three different injection times using a 120min delay-interposed test: 15min before the sample phase (Time I), immediately after the sample phase (Time II), and 15min before the test phase (Time III). The blockade of hippocampal NMDA receptors before and immediately after the sample phase, but not before the test phase, markedly impaired performance of object location recognition test, suggesting that hippocampal NMDA receptors play an important role in encoding and consolidation/retention, but not retrieval, of spontaneous object location memory. Copyright © 2017 Elsevier B.V. All rights reserved.

Complex dark-field contrast and its retrieval in x-ray phase contrast imaging implemented with Talbot interferometry.

PubMed

Yang, Yi; Tang, Xiangyang

2014-10-01

Under the existing theoretical framework of x-ray phase contrast imaging methods implemented with Talbot interferometry, the dark-field contrast refers to the reduction in interference fringe visibility due to small-angle x-ray scattering of the subpixel microstructures of an object to be imaged. This study investigates how an object's subpixel microstructures can also affect the phase of the intensity oscillations. Instead of assuming that the object's subpixel microstructures distribute in space randomly, the authors' theoretical derivation starts by assuming that an object's attenuation projection and phase shift vary at a characteristic size that is not smaller than the period of analyzer grating G₂ and a characteristic length dc. Based on the paraxial Fresnel-Kirchhoff theory, the analytic formulae to characterize the zeroth- and first-order Fourier coefficients of the x-ray irradiance recorded at each detector cell are derived. Then the concept of complex dark-field contrast is introduced to quantify the influence of the object's microstructures on both the interference fringe visibility and the phase of intensity oscillations. A method based on the phase-attenuation duality that holds for soft tissues and high x-ray energies is proposed to retrieve the imaginary part of the complex dark-field contrast for imaging. Through computer simulation study with a specially designed numerical phantom, they evaluate and validate the derived analytic formulae and the proposed retrieval method. Both theoretical analysis and computer simulation study show that the effect of an object's subpixel microstructures on x-ray phase contrast imaging method implemented with Talbot interferometry can be fully characterized by a complex dark-field contrast. The imaginary part of complex dark-field contrast quantifies the influence of the object's subpixel microstructures on the phase of intensity oscillations. Furthermore, at relatively high energies, for soft tissues it can be retrieved for imaging with a method based on the phase-attenuation duality. The analytic formulae derived in this work to characterize the complex dark-field contrast in x-ray phase contrast imaging method implemented with Talbot interferometry are of significance, which may initiate more activities in the research and development of x-ray differential phase contrast imaging for extensive biomedical applications.

Neural bases of prospective memory: a meta-analysis and the "Attention to Delayed Intention" (AtoDI) model.

PubMed

Cona, Giorgia; Scarpazza, Cristina; Sartori, Giuseppe; Moscovitch, Morris; Bisiacchi, Patrizia Silvia

2015-05-01

Remembering to realize delayed intentions is a multi-phase process, labelled as prospective memory (PM), and involves a plurality of neural networks. The present study utilized the activation likelihood estimation method of meta-analysis to provide a complete overview of the brain regions that are consistently activated in each PM phase. We formulated the 'Attention to Delayed Intention' (AtoDI) model to explain the neural dissociation found between intention maintenance and retrieval phases. The dorsal frontoparietal network is involved mainly in the maintenance phase and seems to mediate the strategic monitoring processes, such as the allocation of top-down attention both towards external stimuli, to monitor for the occurrence of the PM cues, and to internal memory contents, to maintain the intention active in memory. The ventral frontoparietal network is recruited in the retrieval phase and might subserve the bottom-up attention captured externally by the PM cues and, internally, by the intention stored in memory. Together with other brain regions (i.e., insula and posterior cingulate cortex), the ventral frontoparietal network would support the spontaneous retrieval processes. The functional contribution of the anterior prefrontal cortex is discussed extensively for each PM phase. Copyright © 2015 Elsevier Ltd. All rights reserved.

Methods for coherent lensless imaging and X-ray wavefront measurements

NASA Astrophysics Data System (ADS)

Guizar Sicairos, Manuel

X-ray diffractive imaging is set apart from other high-resolution imaging techniques (e.g. scanning electron or atomic force microscopy) for its high penetration depth, which enables tomographic 3D imaging of thick samples and buried structures. Furthermore, using short x-ray pulses, it enables the capability to take ultrafast snapshots, giving a unique opportunity to probe nanoscale dynamics at femtosecond time scales. In this thesis we present improvements to phase retrieval algorithms, assess their performance through numerical simulations, and develop new methods for both imaging and wavefront measurement. Building on the original work by Faulkner and Rodenburg, we developed an improved reconstruction algorithm for phase retrieval with transverse translations of the object relative to the illumination beam. Based on gradient-based nonlinear optimization, this algorithm is capable of estimating the object, and at the same time refining the initial knowledge of the incident illumination and the object translations. The advantages of this algorithm over the original iterative transform approach are shown through numerical simulations. Phase retrieval has already shown substantial success in wavefront sensing at optical wavelengths. Although in principle the algorithms can be used at any wavelength, in practice the focus-diversity mechanism that makes optical phase retrieval robust is not practical to implement for x-rays. In this thesis we also describe the novel application of phase retrieval with transverse translations to the problem of x-ray wavefront sensing. This approach allows the characterization of the complex-valued x-ray field in-situ and at-wavelength and has several practical and algorithmic advantages over conventional focused beam measurement techniques. A few of these advantages include improved robustness through diverse measurements, reconstruction from far-field intensity measurements only, and significant relaxation of experimental requirements over other beam characterization approaches. Furthermore, we show that a one-dimensional version of this technique can be used to characterize an x-ray line focus produced by a cylindrical focusing element. We provide experimental demonstrations of the latter at hard x-ray wavelengths, where we have characterized the beams focused by a kinoform lens and an elliptical mirror. In both experiments the reconstructions exhibited good agreement with independent measurements, and in the latter a small mirror misalignment was inferred from the phase retrieval reconstruction. These experiments pave the way for the application of robust phase retrieval algorithms for in-situ alignment and performance characterization of x-ray optics for nanofocusing. We also present a study on how transverse translations help with the well-known uniqueness problem of one-dimensional phase retrieval. We also present a novel method for x-ray holography that is capable of reconstructing an image using an off-axis extended reference in a non-iterative computation, greatly generalizing an earlier approach by Podorov et al. The approach, based on the numerical application of derivatives on the field autocorrelation, was developed from first mathematical principles. We conducted a thorough theoretical study to develop technical and intuitive understanding of this technique and derived sufficient separation conditions required for an artifact-free reconstruction. We studied the effects of missing information in the Fourier domain, and of an imperfect reference, and we provide a signal-to-noise ratio comparison with the more traditional approach of Fourier transform holography. We demonstrated this new holographic approach through proof-of-principle optical experiments and later experimentally at soft x-ray wavelengths, where we compared its performance to Fourier transform holography, iterative phase retrieval and state-of-the-art zone-plate x-ray imaging techniques (scanning and full-field). Finally, we present a demonstration of the technique using a single 20 fs pulse from a high-harmonic table-top source. Holography with an extended reference is shown to provide fast, good quality images that are robust to noise and artifacts that arise from missing information due to a beam stop. (Abstract shortened by UMI.)

The Effects of Emotional Visual Context on the Encoding and Retrieval of Body Odor Information.

PubMed

Parma, Valentina; Macedo, Stephanie; Rocha, Marta; Alho, Laura; Ferreira, Jacqueline; Soares, Sandra C

2018-04-01

Conditions during information encoding and retrieval are known to influence the sensory material stored and its recapitulation. However, little is known about such processes in olfaction. Here, we capitalized on the uniqueness of body odors (BOs) which, similar to fingerprints, allow for the identification of a specific person, by associating their presentation to a negative or a neutral emotional context. One hundred twenty-five receivers (68 F) were exposed to a male BO while watching either criminal or neutral videos (encoding phase) and were subsequently asked to recognize the target BO within either a congruent or an incongruent visual context (retrieval phase). The results showed that criminal videos were rated as more vivid, unpleasant, and arousing than neutral videos both at encoding and retrieval. Moreover, in terms of BO ratings, we found that odor intensity and arousal allow to distinguish the target from the foils when congruent criminal information is presented at encoding and retrieval. Finally, the accuracy performance was not significantly different from chance level for either condition. These findings provide insights on how olfactory memories are processed in emotional situations.

Above-Cloud Precipitable Water Retrievals using the MODIS 0.94 micron Band with Applications for Multi-Layer Cloud Detection

NASA Technical Reports Server (NTRS)

Platnick, S.; Wind, G.

2004-01-01

In order to perform satellite retrievals of cloud properties, it is important to account for the effect of the above-cloud atmosphere on the observations. The solar bands used in the operational MODIS Terra and Aqua cloud optical and microphysical algorithms (visible, NIR, and SWIR spectral windows) are primarily affected by water vapor, and to a lesser extent by well-mixed gases. For water vapor, the above-cloud column amount, or precipitable water, provides adequate information for an atmospheric correction; details of the vertical vapor distribution are not typically necessary for the level of correction required. Cloud-top pressure has a secondary effect due to pressure broadening influences. For well- mixed gases, cloud-top pressure is also required for estimates of above-cloud abundances. We present a method for obtaining above-cloud precipitable water over dark Ocean surfaces using the MODIS 0.94 pm vapor absorption band. The retrieval includes an iterative procedure for establishing cloud-top temperature and pressure, and is useful for both single layer water and ice clouds. Knowledge of cloud thermodynamic phase is fundamental in retrieving cloud optical and microphysical properties. However, in cases of optically thin cirrus overlapping lower water clouds, the concept of a single unique phase is ill- defined and depends, at least, on the spectral region of interest. We will present a method for multi-layer and multi-phase cloud detection which uses above-cloud precipitable water retrievals along with several existing MODIS operational cloud products (cloud-top pressure derived from a C02 slicing algorithm, IR and SWIR phase retrievals). Results are catagorized by whether the radiative signature in the MODIS solar bands is primarily that of a water cloud with ice cloud contamination, or visa-versa. Examples in polar and mid-latitude regions will be shown.

Corrugated Waveguide Mode Content Analysis Using Irradiance Moments

PubMed Central

Jawla, Sudheer K.; Shapiro, Michael A.; Idei, Hiroshi; Temkin, Richard J.

2015-01-01

We present a novel, relatively simple method for determining the mode content of the linearly polarized modes of a corrugated waveguide using the moments of the intensity pattern of the field radiated from the end of the waveguide. This irradiance moment method is based on calculating the low-order irradiance moments, using measured intensity profiles only, of the radiated field from the waveguide aperture. Unlike the phase retrieval method, this method does not use or determine the phase distribution at the waveguide aperture. The new method was benchmarked numerically by comparison with sample mode mixtures. The results predict less than ±0.7% error bar in the retrieval of the mode content. The method was also tested using high-resolution experimental data from beams radiated from 63.5 mm and 19 mm corrugated waveguides at 170 and 250 GHz, respectively. The results showed a very good agreement of the mode content retrieved using the irradiance moment method versus the phase retrieval technique. The irradiance moment method is most suitable for cases where the modal power is primarily in the fundamental HE11 mode, with <8% of the power in high-order modes. PMID:25821260

Quantitative Analysis of the Usage of the COSMOS Science Education Portal

ERIC Educational Resources Information Center

Sotiriou, Sofoklis; Bogner, Franz X.; Neofotistos, George

2011-01-01

A quantitative method of mapping the web usage of an innovative educational portal is applied to analyze the behaviour of users of the COSMOS Science Education Portal. The COSMOS Portal contains user-generated resources (that are uploaded by its users). It has been designed to support a science teacher's search, retrieval and access to both,…

Hybrid Architecture Active Wavefront Sensing and Control

NASA Technical Reports Server (NTRS)

Feinberg, Lee; Dean, Bruce; Hyde, Tupper

2010-01-01

A method was developed for performing relatively high-speed wavefront sensing and control to overcome thermal instabilities in a segmented primary mirror telescope [e.g., James Webb Space Telescope (JWST) at L2], by using the onboard fine guidance sensor (FGS) to minimize expense and complexity. This FGS performs centroiding on a bright star to feed the information to the pointing and control system. The proposed concept is to beam split the image of the guide star (or use a single defocused guide star image) to perform wavefront sensing using phase retrieval techniques. Using the fine guidance sensor star image for guiding and fine phasing eliminates the need for other, more complex ways of achieving very accurate sensing and control that is needed for UV-optical applications. The phase retrieval occurs nearly constantly, so passive thermal stability over fourteen days is not required. Using the FGS as the sensor, one can feed segment update information to actuators on the primary mirror that can update the primary mirror segment fine phasing with this frequency. Because the thermal time constants of the primary mirror are very slow compared to this duration, the mirror will appear extremely stable during observations (to the level of accuracy of the sensing and control). The sensing can use the same phase retrieval techniques as the JWST by employing an additional beam splitter, and having each channel go through a weak lens (one positive and one negative). The channels can use common or separate detectors. Phase retrieval can be performed onboard. The actuation scheme would include a coarse stage able to achieve initial alignment of several millimeters of range (similar to JWST and can use a JWST heritage sensing approach in the science camera) and a fine stage capable of continual updates.

Image inversion analysis of the HST OTA (Hubble Space Telescope Optical Telescope Assembly), phase A

NASA Technical Reports Server (NTRS)

Litvak, M. M.

1991-01-01

Technical work during September-December 1990 consisted of: (1) analyzing HST point source images obtained from JPL; (2) retrieving phase information from the images by a direct (noniterative) technique; and (3) characterizing the wavefront aberration due to the errors in the Hubble Space Telescope (HST) mirrors, in a preliminary manner. This work was in support of JPL design of compensating optics for the next generation wide-field planetary camera on HST. This digital technique for phase retrieval from pairs of defocused images, is based on the energy transport equation between these image planes. In addition, an end-to-end wave optics routine, based on the JPL Code 5 prescription of the unaberrated HST and WFPC, was derived for output of the reference phase front when mirror error is absent. Also, the Roddier routine unwrapped the retrieved phase by inserting the required jumps of +/- 2(pi) radians for the sake of smoothness. A least-squares fitting routine, insensitive to phase unwrapping, but nonlinear, was used to obtain estimates of the Zernike polynomial coefficients that describe the aberration. The phase results were close to, but higher than, the expected error in conic constant of the primary mirror suggested by the fossil evidence. The analysis of aberration contributed by the camera itself could be responsible for the small discrepancy, but was not verified by analysis.

Investigating the Capability to Extract Impulse Response Functions From Ambient Seismic Noise Using a Mine Collapse Event

NASA Astrophysics Data System (ADS)

Kwak, Sangmin; Song, Seok Goo; Kim, Geunyoung; Cho, Chang Soo; Shin, Jin Soo

2017-10-01

Using recordings of a mine collapse event (Mw 4.2) in South Korea in January 2015, we demonstrated that the phase and amplitude information of impulse response functions (IRFs) can be effectively retrieved using seismic interferometry. This event is equivalent to a single downward force at shallow depth. Using quantitative metrics, we compared three different seismic interferometry techniques—deconvolution, coherency, and cross correlation—to extract the IRFs between two distant stations with ambient seismic noise data. The azimuthal dependency of the source distribution of the ambient noise was also evaluated. We found that deconvolution is the best method for extracting IRFs from ambient seismic noise within the period band of 2-10 s. The coherency method is also effective if appropriate spectral normalization or whitening schemes are applied during the data processing.

Circadian waveform bifurcation, but not phase-shifting, leaves cued fear memory intact.

PubMed

Harrison, E M; Carmack, S A; Block, C L; Sun, J; Anagnostaras, S G; Gorman, M R

2017-02-01

In mammals, memory acquisition and retrieval can be affected by time of day, as well as by manipulations of the light/dark cycle. Under bifurcation, a manipulation of circadian waveform, two subjective days and nights are experimentally induced in rodents. We examined the effect of bifurcation on Pavlovian fear conditioning, a prominent model of learning and memory. Here we demonstrate that bifurcation of the circadian waveform produces a small deficit in acquisition, but not on retrieval of fear memory. In contrast, repeated phase-shifting in a simulated jet-lag protocol impairs retrieval of memory for cued fear. The results have implications for those attempting to adjust to shift-work or other challenging schedules. Copyright © 2016 Elsevier Inc. All rights reserved.

Quantitative and qualitative analysis of study-related patient information sheets in randomised neuro-oncology phase III-trials.

PubMed

Reinert, Christiane; Kremmler, Lukas; Burock, Susen; Bogdahn, Ulrich; Wick, Wolfgang; Gleiter, Christoph H; Koller, Michael; Hau, Peter

2014-01-01

In randomised controlled trials (RCTs), patient informed consent documents are an essential cornerstone of the study flow. However, these documents are often oversized in format and content. Clinical experience suggests that study information sheets are often not used as an aid to decision-making due to their complexity. We analysed nine patient informed consent documents from clinical neuro-oncological phase III-studies running at a German Brain Tumour Centre with the objective to investigate the quality of these documents. Text length, formal layout, readability, application of ethical and legal requirements, scientific evidence and social aspects were used as rating categories. Results were assessed quantitatively by two independents investigators and were depicted using net diagrams. All patient informed consent documents were of insufficient quality in all categories except that ethical and legal requirements were fulfilled. Notably, graduate levels were required to read and understand five of nine consent documents. Quality deficits were consistent between the individual study information texts. Irrespective of formal aspects, a document that is intended to inform and motivate patients to participate in a study needs to be well-structured and understandable. We therefore strongly mandate to re-design patient informed consent documents in a patient-friendly way. Specifically, standardised components with a scientific foundation should be provided that could be retrieved at various times, adapted to the mode of treatment and the patient's knowledge, and could weigh information dependent of the stage of treatment decision. Copyright © 2013 Elsevier Ltd. All rights reserved.

Quantitative genetics of secondary hip joint osteoarthritis in a Labrador Retriever-Greyhound pedigree.

PubMed

Hays, Laurel; Zhang, Zhiwu; Mateescu, Raluca G; Lust, George; Burton-Wurster, Nancy I; Todhunter, Rory J

2007-01-01

To evaluate the quantitative inheritance of secondary hip joint osteoarthritis in a canine pedigree. 137 Labrador Retrievers, Greyhounds, and mixed-breed dogs. Necropsy scores ranging from 0 to 4 were obtained for each hip joint. Seven unaffected Greyhounds with normal hip joint conformation were also used for genetic modeling, but were not euthanized. Sixty-six male and 71 female dogs were allocated to 2 groups (< or = 12 months of age and > 12 months of age). Statistical models were developed to establish the inheritance pattern of hip joint osteoarthritis that developed secondary to hip dysplasia. 62 dogs had evidence of osteoarthritis in a hip joint, and 75 had no evidence of osteoarthritis. After sex was adjusted for, the necropsy score was found to be inherited additively but without dominance. Each Labrador Retriever allele increased the necropsy score by 0.7 to 0.9 points, compared with the Greyhound allele, and male sex increased the necropsy score 0.74 over female sex. Approximately 10% of the variation in necropsy score was attributable to the litter of puppies' origin. Because secondary hip joint osteoarthritis is inherited additively, selection pressure could be applied to reduce its incidence. Similar statistical models can be used in linkage and association mapping to detect the genes in the underlying quantitative trait loci that contribute to hip joint osteoarthritis.

Forty-photon-per-pulse spectral phase retrieval by shaper-assisted modified interferometric field autocorrelation.

PubMed

Hsu, Chen-Shao; Chiang, Hsin-Chien; Chuang, Hsiu-Po; Huang, Chen-Bin; Yang, Shang-Da

2011-07-15

We retrieve the spectral phase of 400 fs pulses at 1560 nm with 5.2 aJ coupled pulse energy (40 photons) by the modified interferometric field autocorrelation method, using a pulse shaper and a 5 cm long periodically poled lithium niobate waveguide. The carrier-envelope phase control of the shaper can reduce the fringe density of the interferometric trace and permits longer lock-in time constants, achieving a sensitivity of 2.7×10(-9) mW(2) (40 times better than the previous record for self-referenced nonlinear pulse measurement). The high stability of the pulse shaper allows for accurate and reproducible measurements of complicated spectral phases. © 2011 Optical Society of America

Conjugate gradient method for phase retrieval based on the Wirtinger derivative.

PubMed

Wei, Zhun; Chen, Wen; Qiu, Cheng-Wei; Chen, Xudong

2017-05-01

A conjugate gradient Wirtinger flow (CG-WF) algorithm for phase retrieval is proposed in this paper. It is shown that, compared with recently reported Wirtinger flow and its modified methods, the proposed CG-WF algorithm is able to dramatically accelerate the convergence rate while keeping the dominant computational cost of each iteration unchanged. We numerically illustrate the effectiveness of our method in recovering 1D Gaussian signals and 2D natural color images under both Gaussian and coded diffraction pattern models.

Phase retrieval with Fourier-weighted projections.

PubMed

Guizar-Sicairos, Manuel; Fienup, James R

2008-03-01

In coherent lensless imaging, the presence of image sidelobes, which arise as a natural consequence of the finite nature of the detector array, was early recognized as a convergence issue for phase retrieval algorithms that rely on an object support constraint. To mitigate the problem of truncated far-field measurement, a controlled analytic continuation by means of an iterative transform algorithm with weighted projections is proposed and tested. This approach avoids the use of sidelobe reduction windows and achieves full-resolution reconstructions.

Image multiplexing and authentication based on double phase retrieval in fresnel transform domain

NASA Astrophysics Data System (ADS)

Chang, Hsuan-Ting; Lin, Che-Hsian; Chen, Chien-Yue

2017-04-01

An image multiplexing and authentication method based on the double-phase retrieval algorithm (DPRA) with the manipulations of wavelength and position in the Fresnel transform (FrT) domain is proposed in this study. The DPRA generates two matched phase-only functions (POFs) in the different planes so that the corresponding image can be reconstructed at the output plane. Given a number of target images, all the sets of matched POFs are used to generate the phase-locked system through the phase modulation and synthesis to achieve the multiplexing purpose. To reconstruct a target image, the corresponding phase key and all the correct parameters in the FrT are required. Therefore, the authentication system with high-level security can be achieved. The computer simulation verifies the validity of the proposed method and also shows good resistance to the crosstalk among the reconstructed images.

Using sparsity information for iterative phase retrieval in x-ray propagation imaging.

PubMed

Pein, A; Loock, S; Plonka, G; Salditt, T

2016-04-18

For iterative phase retrieval algorithms in near field x-ray propagation imaging experiments with a single distance measurement, it is indispensable to have a strong constraint based on a priori information about the specimen; for example, information about the specimen's support. Recently, Loock and Plonka proposed to use the a priori information that the exit wave is sparsely represented in a certain directional representation system, a so-called shearlet system. In this work, we extend this approach to complex-valued signals by applying the new shearlet constraint to amplitude and phase separately. Further, we demonstrate its applicability to experimental data.

On-Line Retrieval II.

ERIC Educational Resources Information Center

Kurtz, Peter; And Others

This report is concerned with the implementation of two interrelated computer systems: an automatic document analysis and classification package, and an on-line interactive information retrieval system which utilizes the information gathered during the automatic classification phase. Well-known techniques developed by Salton and Dennis have been…

A Ground-Based Doppler Radar and Micropulse Lidar Forward Simulator for GCM Evaluation of Arctic Mixed-Phase Clouds: Moving Forward Towards an Apples-to-apples Comparison of Hydrometeor Phase

NASA Astrophysics Data System (ADS)

Lamer, K.; Fridlind, A. M.; Ackerman, A. S.; Kollias, P.; Clothiaux, E. E.

2017-12-01

An important aspect of evaluating Artic cloud representation in a general circulation model (GCM) consists of using observational benchmarks which are as equivalent as possible to model output in order to avoid methodological bias and focus on correctly diagnosing model dynamical and microphysical misrepresentations. However, current cloud observing systems are known to suffer from biases such as limited sensitivity, and stronger response to large or small hydrometeors. Fortunately, while these observational biases cannot be corrected, they are often well understood and can be reproduced in forward simulations. Here a ground-based millimeter wavelength Doppler radar and micropulse lidar forward simulator able to interface with output from the Goddard Institute for Space Studies (GISS) ModelE GCM is presented. ModelE stratiform hydrometeor fraction, mixing ratio, mass-weighted fall speed and effective radius are forward simulated to vertically-resolved profiles of radar reflectivity, Doppler velocity and spectrum width as well as lidar backscatter and depolarization ratio. These forward simulated fields are then compared to Atmospheric Radiation Measurement (ARM) North Slope of Alaska (NSA) ground-based observations to assess cloud vertical structure (CVS). Model evalution of Arctic mixed-phase cloud would also benefit from hydrometeor phase evaluation. While phase retrieval from synergetic observations often generates large uncertainties, the same retrieval algorithm can be applied to observed and forward-simulated radar-lidar fields, thereby producing retrieved hydrometeor properties with potentially the same uncertainties. Comparing hydrometeor properties retrieved in exactly the same way aims to produce the best apples-to-apples comparisons between GCM ouputs and observations. The use of a comprenhensive ground-based forward simulator coupled with a hydrometeor classification retrieval algorithm provides a new perspective for GCM evaluation of Arctic mixed-phase clouds from the ground where low-level supercooled liquid layer are more easily observed and where additional environmental properties such as cloud condensation nuclei are quantified. This should help assist in choosing between several possible diagnostic ice nucleation schemes for ModelE stratiform cloud.

Self control of when and how much to test face-name pairs in a novel spaced retrieval paradigm: an examination of age-related differences.

PubMed

Maddox, Geoffrey B; Balota, David A

2012-09-01

Although the mnemonic benefit of spaced retrieval is well established, the way in which participants naturally space their own retrieval is relatively unexplored. To examine this question, a novel experimental paradigm was developed in which young and healthy older adults were given control over the frequency and timing of retrieval practice in the context of an ongoing reading task. Results showed that both age groups naturally expanded the intervals of their retrieval practice. When instructed, younger adults but not older adults were better able to employ equal spaced retrieval during retrieval practice. However, even under equal spaced retrieval instructions, young adults included an early retrieval attempt prior to equally spacing their retrieval. Although memory performance was equivalent, secondary task performance was reduced in the experimenter-instructed condition compared with the participant-selected condition. The results overall indicate that both younger and older participants naturally monitor their memory and efficiently use testing to titrate the number and timing of retrieval attempts used during the acquisition phase.

Retrieval of aerosol optical and micro-physical properties with 2D-MAX-DOAS

NASA Astrophysics Data System (ADS)

Ortega, Ivan; Coburn, Sean; Hostetler, Chris; Ferrare, Rich; Hair, Johnathan; Kassianov, Evgueni; Barnard, James; Berg, Larry; Schmid, Beat; Tomlinson, Jason; Hodges, Gary; Lantz, Kathy; Wagner, Thomas; Volkamer, Rainer

2015-04-01

Recent retrievals of 2 dimensional (2D) Multi-AXis Differential Optical Absorption Spectroscopy (2D-MAX-DOAS) have highlighted its importance in order to infer diurnal horizontal in-homogeneities around the measurement site. In this work, we expand the capabilities of 2D measurements in order to estimate simultaneously aerosol optical and micro-physical properties. Specifically, we present a retrieval method to obtain: (1) aerosol optical thickness (AOT) in the boundary layer (BL) and free troposphere (FT) and (2) the effective complex refractive index and the effective radius of the aerosol column size distribution. The retrieval method to obtain AOT is based on an iterative comparison of measured normalized radiances, oxygen collision pair (O4), and absolute Raman Scattering Probability (RSP) with the forward model calculations derived with the radiative transfer model McArtim based on defined aerosol extinction profiles. Once the aerosol load is determined we use multiple scattering phase functions and single scattering albedo (SSA) obtained with Mie calculations which then constrain the RTM to forward model solar almucantar normalized radiances. The simulated almucantar normalized radiances are then compared to the measured normalized radiances. The best-fit, determined by minimizing the root mean square, retrieves the complex refractive index, and effective radius. We apply the retrieval approach described above to measurements carried out during the 2012 intensive operation period of the Two Column Aerosol Project (TCAP) held on Cape Cod, MA, USA. Results are presented for two ideal case studies with both large and small aerosol loading and similar air mass outflow from the northeast coast of the US over the West Atlantic Ocean. The aerosol optical properties are compared with several independent instruments, including the NASA Langley airborne High Spectral Resolution Lidar (HSRL-2) for highly resolved extinction profiles during the overpasses, and with the co-located Multi Filter Rotating Shadow band Radiometer (MFRSR), and the Cimel Sun photometer for aerosol load at several wavelengths. To test aerosol horizontal homogeneity we use quantitatively analysis of asymmetry of solar azimuth normalized radiances and RSP. The aerosol column microphysical properties will be compared with merged size distribution of several in-situ instruments from airborne measurements during overpasses of the DoE-G1 aircraft around the ground measurement site.

Evaluation of applicability of high-resolution multiangle imaging photo-polarimetric observations for aerosol atmospheric correction

NASA Astrophysics Data System (ADS)

Kalashnikova, Olga; Garay, Michael; Xu, Feng; Diner, David; Seidel, Felix

2016-07-01

Multiangle spectro-polarimetric measurements have been advocated as an additional tool for better understanding and quantifying the aerosol properties needed for atmospheric correction for ocean color retrievals. The central concern of this work is the assessment of the effects of absorbing aerosol properties on remote sensing reflectance measurement uncertainty caused by neglecting UV-enhanced absorption of carbonaceous particles and by not accounting for dust nonsphericity. In addition, we evaluate the polarimetric sensitivity of absorbing aerosol properties in light of measurement uncertainties achievable for the next generation of multi-angle polarimetric imaging instruments, and demonstrate advantages and disadvantages of wavelength selection in the UV/VNIR range. In this work a vector Markov Chain radiative transfer code including bio-optical models was used to quantitatively evaluate in water leaving radiances between atmospheres containing realistic UV-enhanced and non-spherical aerosols and the SEADAS carbonaceous and dust-like aerosol models. The phase matrices for the spherical smoke particles were calculated using a standard Mie code, while those for non-spherical dust particles were calculated using the numerical approach developed for modeling dust for the AERONET network of ground-based sunphotometers. As a next step, we have developed a retrieval code that employs a coupled Markov Chain (MC) and adding/doubling radiative transfer method for joint retrieval of aerosol properties and water leaving radiance from Airborne Multiangle SpectroPolarimetric Imager-1 (AirMSPI-1) polarimetric observations. The AirMSPI-1 instrument has been flying aboard the NASA ER-2 high altitude aircraft since October 2010. AirMSPI typically acquires observations of a target area at 9 view angles between ±67° at 10 m resolution. AirMSPI spectral channels are centered at 355, 380, 445, 470, 555, 660, and 865 nm, with 470, 660, and 865 reporting linear polarization. We tested prototype retrievals by comparing the retrieved aerosol concentration, size distribution, water-leaving radiance, and chlorophyll concentrations from Airborne Multiangle SpectroPolarimetric Imager-1 (AirMSPI-1) observations to values reported by the USC SeaPRISM AERONET-OC site off the coast of California. The retrieval was then applied to a variety of costal regions in California to evaluate variability in the water-leaving radiance under different atmospheric conditions. We will present results, and will discuss algorithm sensitivity and potential applications for future space-borne coastal monitoring.

rTMS stimulation on left DLPFC affects emotional cue retrieval as a function of anxiety level and gender.

PubMed

Balconi, Michela; Ferrari, Chiara

2012-11-01

Anxiety behaviour showed a consistent attentional bias toward negative and aversive memories, induced by a right dorsolateral prefrontal cortex (DLPFC) hyperactivation. In the present research, we explored the possible effect of rTMS (repeated transcranial magnetic stimulation) on the left DLPFC in memory retrieval of positive versus negative emotional words, to induce a balanced response between the two hemispheres. Moreover, the gender effect in emotional memory processing was verified as a function of the stimulus valence. Thirty subjects, who were divided in two different groups depending on their anxiety level (high/low anxiety, State-Trait-Anxiety Inventory (STAI)), were required to perform a task consisting of two experimental phases: an encoding phase (lists composed by positive and negative emotional words); and a retrieval phase (old stimuli and new stimuli to be recognized). We found that the rTMS stimulation over left DLPFC affects the memory retrieval. Specifically, high-anxiety subjects benefitted in greater measure to the frontal left stimulation with a reduced negative bias (increased accuracy and reduced response time (RT) for the positive stimuli). Whereas females showed a significant bias toward the negative memories, they did not benefit in greater measure to the TMS stimulation on the left hemisphere. These results suggested that left DLPFC activation favors the memory retrieval of positive emotional information and may limit the "unbalance effect" induced by a right frontal hemispheric superiority in high levels of anxiety. © 2012 Wiley Periodicals, Inc.

Initial retrieval shields against retrieval-induced forgetting.

PubMed

Racsmány, Mihály; Keresztes, Attila

2015-01-01

Testing, as a form of retrieval, can enhance learning but it can also induce forgetting of related memories, a phenomenon known as retrieval-induced forgetting (RIF). In four experiments we explored whether selective retrieval and selective restudy of target memories induce forgetting of related memories with or without initial retrieval of the entire learning set. In Experiment 1, subjects studied category-exemplar associations, some of which were then either restudied or retrieved. RIF occurred on a delayed final test only when memories were retrieved and not when they were restudied. In Experiment 2, following the study phase of category-exemplar associations, subjects attempted to recall all category-exemplar associations, then they selectively retrieved or restudied some of the exemplars. We found that, despite the huge impact on practiced items, selective retrieval/restudy caused no decrease in final recall of related items. In Experiment 3, we replicated the main result of Experiment 2 by manipulating initial retrieval as a within-subject variable. In Experiment 4 we replicated the main results of the previous experiments with non-practiced (Nrp) baseline items. These findings suggest that initial retrieval of the learning set shields against the forgetting effect of later selective retrieval. Together, our results support the context shift theory of RIF.

Retrieval-practice task affects relationship between working memory capacity and retrieval-induced forgetting.

PubMed

Storm, Benjamin C; Bui, Dung C

2016-11-01

Retrieving a subset of items from memory can cause forgetting of other items in memory, a phenomenon referred to as retrieval-induced forgetting (RIF). Individuals who exhibit greater amounts of RIF have been shown to also exhibit superior working memory capacity (WMC) and faster stop-signal reaction times (SSRTs), results which have been interpreted as suggesting that RIF reflects an inhibitory process that is mediated by the processes of executive control. Across four experiments, we sought to further elucidate this issue by manipulating the way in which participants retrieved items during retrieval practice and examining how the resulting effects of forgetting correlated with WMC (Experiments 1-3) and SSRT (Experiment 4). Significant correlations were observed when participants retrieved items from an earlier study phase (within-list retrieval practice), but not when participants generated items from semantic memory (extra-list retrieval practice). These results provide important new insight into the role of executive-control processes in RIF.

Phase Retrieval System for Assessing Diamond Turning and Optical Surface Defects

NASA Technical Reports Server (NTRS)

Dean, Bruce; Maldonado, Alex; Bolcar, Matthew

2011-01-01

An optical design is presented for a measurement system used to assess the impact of surface errors originating from diamond turning artifacts. Diamond turning artifacts are common by-products of optical surface shaping using the diamond turning process (a diamond-tipped cutting tool used in a lathe configuration). Assessing and evaluating the errors imparted by diamond turning (including other surface errors attributed to optical manufacturing techniques) can be problematic and generally requires the use of an optical interferometer. Commercial interferometers can be expensive when compared to the simple optical setup developed here, which is used in combination with an image-based sensing technique (phase retrieval). Phase retrieval is a general term used in optics to describe the estimation of optical imperfections or aberrations. This turnkey system uses only image-based data and has minimal hardware requirements. The system is straightforward to set up, easy to align, and can provide nanometer accuracy on the measurement of optical surface defects.

Noniterative approach to the missing data problem in coherent diffraction imaging by phase retrieval.

PubMed

Nakajima, Nobuharu

2010-07-20

When a very intense beam is used for illuminating an object in coherent x-ray diffraction imaging, the intensities at the center of the diffraction pattern for the object are cut off by a beam stop that is utilized to block the intense beam. Until now, only iterative phase-retrieval methods have been applied to object reconstruction from a single diffraction pattern with a deficiency of central data due to a beam stop. As an alternative method, I present a noniterative solution in which an interpolation method based on the sampling theorem for the missing data is used for object reconstruction with our previously proposed phase-retrieval method using an aperture-array filter. Computer simulations demonstrate the reconstruction of a complex-amplitude object from a single diffraction pattern with a missing data area, which is generally difficult to treat with the iterative methods because a nonnegativity constraint cannot be used for such an object.

Gram-Schmidt orthonormalization for retrieval of amplitude images under sinusoidal patterns of illumination

USDA-ARS?s Scientific Manuscript database

Structured illumination using sinusoidal patterns has been utilized for optical imaging of biological tissues in biomedical research and, of horticultural products. Implementation of structured-illumination imaging relies on retrieval of amplitude images, which is conventionally achieved by a phase-...

Brain Activity During the Encoding, Retention, and Retrieval of Stimulus Representations

PubMed Central

de Zubicaray, Greig I.; McMahon, Katie; Wilson, Stephen J.; Muthiah, Santhi

2001-01-01

Studies of delayed nonmatching-to-sample (DNMS) performance following lesions of the monkey cortex have revealed a critical circuit of brain regions involved in forming memories and retaining and retrieving stimulus representations. Using event-related functional magnetic resonance imaging (fMRI), we measured brain activity in 10 healthy human participants during performance of a trial-unique visual DNMS task using novel barcode stimuli. The event-related design enabled the identification of activity during the different phases of the task (encoding, retention, and retrieval). Several brain regions identified by monkey studies as being important for successful DNMS performance showed selective activity during the different phases, including the mediodorsal thalamic nucleus (encoding), ventrolateral prefrontal cortex (retention), and perirhinal cortex (retrieval). Regions showing sustained activity within trials included the ventromedial and dorsal prefrontal cortices and occipital cortex. The present study shows the utility of investigating performance on tasks derived from animal models to assist in the identification of brain regions involved in human recognition memory. PMID:11584070

Spatio-temporal phase retrieval in speckle interferometry with Hilbert transform and two-dimensional phase unwrapping

NASA Astrophysics Data System (ADS)

Li, Xiangyu; Huang, Zhanhua; Zhu, Meng; He, Jin; Zhang, Hao

2014-12-01

Hilbert transform (HT) is widely used in temporal speckle pattern interferometry, but errors from low modulations might propagate and corrupt the calculated phase. A spatio-temporal method for phase retrieval using temporal HT and spatial phase unwrapping is presented. In time domain, the wrapped phase difference between the initial and current states is directly determined by using HT. To avoid the influence of the low modulation intensity, the phase information between the two states is ignored. As a result, the phase unwrapping is shifted from time domain to space domain. A phase unwrapping algorithm based on discrete cosine transform is adopted by taking advantage of the information in adjacent pixels. An experiment is carried out with a Michelson-type interferometer to study the out-of-plane deformation field. High quality whole-field phase distribution maps with different fringe densities are obtained. Under the experimental conditions, the maximum number of fringes resolvable in a 416×416 frame is 30, which indicates a 15λ deformation along the direction of loading.

Ca2Mg(NO3)6×12H2O-structural investigations on a new compound retrieved from chimney deposits of a combined heat and power plant

NASA Astrophysics Data System (ADS)

Kahlenberg, Volker; Tessadri, Richard; Tribus, Martina; Schmidmair, Daniela; Perfler, Lukas; Haefeker, Udo

2014-10-01

Phase analysis of incrustations retrieved from chimney deposits of a combined heat and power plant in Malchow/Germany by X-ray powder diffraction gave evidence for the existence of a previously unknown hydrous calcium magnesium nitrate. Optical investigations of the sample showed the presence of colorless platy crystals with a maximum diameter of about 250 μm embedded in a partly polycrystalline and partly glassy matrix. Aided by EDX-analysis and Raman spectroscopy, a single-crystal diffraction study performed at ambient conditions demonstrated that the material represents a phase with composition Ca2Mg(NO3)6×12H2O. Basic crystallographic data are as follows: trigonal symmetry, space group type R , a = 10.5583(5) Å, c = 19.5351(10) Å, V = 1885.97(16) Å3, Z = 3, ( R(| F|) = 0.0248). The magnesium ions are coordinated by water molecules to form distorted Mg(H2O)6-octahedra. The calcium atoms are surrounded by nine ligands. The resulting CaO9 tricapped trigonal prisms involve oxygen atoms from additional water moieties as well as from three different bidentate nitrate groups, respectively. Hydrogen bonds link one octahedron with two adjacent prisms into trimers. The trimers in turn are stacked in columns running parallel to [001]. Further hydrogen bonding between neighboring columns results in the formation of a three-dimensional network. To our best knowledge, Ca2Mg(NO3)6×12H2O represents a new structure type. However, column-like topologies with rods consisting of different types of polyhedra have been also observed in other trigonal hydrous nitrates. The structural relationships between these compounds are discussed. It is interesting to note that in previous phase equilibrium studies on the ternary system Ca(NO3)2-Mg(NO3)2-H2O no other hydrous double salt has been described. Finally, the results of the structure analysis allowed a qualitative and quantitative phase analysis of the crystalline part of the chimney deposit by the Rietveld method.

Stress within a Restricted Time Window Selectively Affects the Persistence of Long-Term Memory

PubMed Central

Fang, Qin; Chai, Ning; Zhao, Li-Yan; Xue, Yan-Xue; Luo, Yi-Xiao; Jian, Min; Han, Ying; Shi, Hai-Shui; Lu, Lin; Wu, Ping; Wang, Ji-Shi

2013-01-01

The effects of stress on emotional memory are distinct and depend on the stages of memory. Memory undergoes consolidation and reconsolidation after acquisition and retrieval, respectively. Stress facilitates the consolidation but disrupts the reconsolidation of emotional memory. Previous research on the effects of stress on memory have focused on long-term memory (LTM) formation (tested 24 h later), but the effects of stress on the persistence of LTM (tested at least 1 week later) are unclear. Recent findings indicated that the persistence of LTM requires late-phase protein synthesis in the dorsal hippocampus. The present study investigated the effect of stress (i.e., cold water stress) during the late phase after the acquisition and retrieval of contextual fear memory in rats. We found that stress and corticosterone administration during the late phase (12 h) after acquisition, referred to as late consolidation, selectively enhanced the persistence of LTM, whereas stress during the late phase (12 h) after retrieval, referred to as late reconsolidation, selectively disrupted the restabilized persistence of LTM. Moreover, the effects of stress on the persistence of LTM were blocked by the corticosterone synthesis inhibitor metyrapone, which was administered before stress, suggesting that the glucocorticoid system is involved in the effects of stress on the persistence of LTM. We conclude that stress within a restricted time window after acquisition or retrieval selectively affects the persistence of LTM and depends on the glucocorticoid system. PMID:23544051

A statistical retrieval of cloud parameters for the millimeter wave Ice Cloud Imager on board MetOp-SG

NASA Astrophysics Data System (ADS)

Prigent, Catherine; Wang, Die; Aires, Filipe; Jimenez, Carlos

2017-04-01

The meteorological observations from satellites in the microwave domain are currently limited to below 190 GHz. However, the next generation of European Organisation for the Exploitation of Meteorological Satellites (EUMETSAT) Polar System-Second Generation-EPS-SG will carry an instrument, the Ice Cloud Imager (ICI), with frequencies up to 664 GHz, to improve the characterization of the cloud frozen phase. In this paper, a statistical retrieval of cloud parameters for ICI is developed, trained on a synthetic database derived from the coupling of a mesoscale cloud model and radiative transfer calculations. The hydrometeor profiles simulated with the Weather Research and Forecasting model (WRF) for twelve diverse European mid-latitude situations are used to simulate the brightness temperatures with the Atmospheric Radiative Transfer Simulator (ARTS) to prepare the retrieval database. The WRF+ARTS simulations have been compared to the Special Sensor Microwave Imager/Sounder (SSMIS) observations up to 190 GHz: this successful evaluation gives us confidence in the simulations at the ICI channels from 183 to 664 GHz. Statistical analyses have been performed on this simulated retrieval database, showing that it is not only physically realistic but also statistically satisfactory for retrieval purposes. A first Neural Network (NN) classifier is used to detect the cloud presence. A second NN is developed to retrieve the liquid and ice integrated cloud quantities over sea and land separately. The detection and retrieval of the hydrometeor quantities (i.e., ice, snow, graupel, rain, and liquid cloud) are performed with ICI-only, and with ICI combined with observations from the MicroWave Imager (MWI, with frequencies from 19 to 190 GHz, also on board MetOp-SG). The ICI channels have been optimized for the detection and quantification of the cloud frozen phases: adding the MWI channels improves the performance of the vertically integrated hydrometeor contents, especially for the cloud liquid phases. The relative error for the retrieved integrated frozen water content (FWP, i.e., ice+snow+graupel) is below 40% for 0.1kg/m2 < FWP < 0.5kg/m2 and below 20% for FWP > 0.5 kg/m2.

Optimization of propagation-based x-ray phase-contrast tomography for breast cancer imaging

NASA Astrophysics Data System (ADS)

Baran, P.; Pacile, S.; Nesterets, Y. I.; Mayo, S. C.; Dullin, C.; Dreossi, D.; Arfelli, F.; Thompson, D.; Lockie, D.; McCormack, M.; Taba, S. T.; Brun, F.; Pinamonti, M.; Nickson, C.; Hall, C.; Dimmock, M.; Zanconati, F.; Cholewa, M.; Quiney, H.; Brennan, P. C.; Tromba, G.; Gureyev, T. E.

2017-03-01

The aim of this study was to optimise the experimental protocol and data analysis for in-vivo breast cancer x-ray imaging. Results are presented of the experiment at the SYRMEP beamline of Elettra Synchrotron using the propagation-based phase-contrast mammographic tomography method, which incorporates not only absorption, but also x-ray phase information. In this study the images of breast tissue samples, of a size corresponding to a full human breast, with radiologically acceptable x-ray doses were obtained, and the degree of improvement of the image quality (from the diagnostic point of view) achievable using propagation-based phase-contrast image acquisition protocols with proper incorporation of x-ray phase retrieval into the reconstruction pipeline was investigated. Parameters such as the x-ray energy, sample-to-detector distance and data processing methods were tested, evaluated and optimized with respect to the estimated diagnostic value using a mastectomy sample with a malignant lesion. The results of quantitative evaluation of images were obtained by means of radiological assessment carried out by 13 experienced specialists. A comparative analysis was performed between the x-ray and the histological images of the specimen. The results of the analysis indicate that, within the investigated range of parameters, both the objective image quality characteristics and the subjective radiological scores of propagation-based phase-contrast images of breast tissues monotonically increase with the strength of phase contrast which in turn is directly proportional to the product of the radiation wavelength and the sample-to-detector distance. The outcomes of this study serve to define the practical imaging conditions and the CT reconstruction procedures appropriate for low-dose phase-contrast mammographic imaging of live patients at specially designed synchrotron beamlines.

Expected Improvements in the Quantitative Remote Sensing of Optically Complex Waters with the Use of an Optically Fast Hyperspectral Spectrometer—A Modeling Study

PubMed Central

Moses, Wesley J.; Bowles, Jeffrey H.; Corson, Michael R.

2015-01-01

Using simulated data, we investigated the effect of noise in a spaceborne hyperspectral sensor on the accuracy of the atmospheric correction of at-sensor radiances and the consequent uncertainties in retrieved water quality parameters. Specifically, we investigated the improvement expected as the F-number of the sensor is changed from 3.5, which is the smallest among existing operational spaceborne hyperspectral sensors, to 1.0, which is foreseeable in the near future. With the change in F-number, the uncertainties in the atmospherically corrected reflectance decreased by more than 90% across the visible-near-infrared spectrum, the number of pixels with negative reflectance (caused by over-correction) decreased to almost one-third, and the uncertainties in the retrieved water quality parameters decreased by more than 50% and up to 92%. The analysis was based on the sensor model of the Hyperspectral Imager for the Coastal Ocean (HICO) but using a 30-m spatial resolution instead of HICO’s 96 m. Atmospheric correction was performed using Tafkaa. Water quality parameters were retrieved using a numerical method and a semi-analytical algorithm. The results emphasize the effect of sensor noise on water quality parameter retrieval and the need for sensors with high Signal-to-Noise Ratio for quantitative remote sensing of optically complex waters. PMID:25781507

Optimal Design for Hetero-Associative Memory: Hippocampal CA1 Phase Response Curve and Spike-Timing-Dependent Plasticity

PubMed Central

Miyata, Ryota; Ota, Keisuke; Aonishi, Toru

2013-01-01

Recently reported experimental findings suggest that the hippocampal CA1 network stores spatio-temporal spike patterns and retrieves temporally reversed and spread-out patterns. In this paper, we explore the idea that the properties of the neural interactions and the synaptic plasticity rule in the CA1 network enable it to function as a hetero-associative memory recalling such reversed and spread-out spike patterns. In line with Lengyel’s speculation (Lengyel et al., 2005), we firstly derive optimally designed spike-timing-dependent plasticity (STDP) rules that are matched to neural interactions formalized in terms of phase response curves (PRCs) for performing the hetero-associative memory function. By maximizing object functions formulated in terms of mutual information for evaluating memory retrieval performance, we search for STDP window functions that are optimal for retrieval of normal and doubly spread-out patterns under the constraint that the PRCs are those of CA1 pyramidal neurons. The system, which can retrieve normal and doubly spread-out patterns, can also retrieve reversed patterns with the same quality. Finally, we demonstrate that purposely designed STDP window functions qualitatively conform to typical ones found in CA1 pyramidal neurons. PMID:24204822

Spatial and temporal dynamics of cortical networks engaged in memory encoding and retrieval

PubMed Central

Miller, Brian T.; D'Esposito, Mark

2012-01-01

Memory operations such as encoding and retrieval require the coordinated interplay of cortical regions with distinct functional contributions. The mechanistic nature of these interactions, however, remains unspecified. During the performance of a face memory task during fMRI scanning, we measured the magnitude (a measure of the strength of coupling between areas) and phase (a measure of the relative timing across areas) of coherence between regions of interest and the rest of the brain. The fusiform face area (FFA) showed robust coherence with a distributed network of subregions in the prefrontal cortex (PFC), posterior parietal cortex (PPC), precuneus, and hippocampus across both memory operations. While these findings reveal significant overlap in the cortical networks underlying mnemonic encoding and retrieval, coherence phase analyses revealed context-dependent differences in cortical dynamics. During both encoding and retrieval, PFC and PPC exhibited earlier activity than in the FFA and hippocampus. Also, during retrieval, PFC activity preceded PPC activity. These findings are consistent with prior physiology studies suggesting an early contribution of PFC and PPC in mnemonic control. Together, these findings contribute to the growing literature exploring the spatio-temporal dynamics of basic memory operations. PMID:22557959

Evaluation of AIRS cloud properties using MPACE data

NASA Astrophysics Data System (ADS)

Wu, Xuebao; Li, Jun; Menzel, W. Paul; Huang, Allen; Baggett, Kevin; Revercomb, Henry

2005-12-01

Retrieval of cloud properties from the Atmospheric Infrared Sounder (AIRS) aboard the NASA Aqua satellite has been investigated. The cloud products from the collocated MODerate resolution Imaging Spectroradiometer (MODIS) data are used to characterize the AIRS sub-pixel cloud information such as cloud phase, cloud coverage, and cloud layer information. A Minimum Residual (MR) approach is used to retrieve cloud microphysical properties once the cloud top pressure (CTP) and effective cloud amount (ECA) are determined from AIRS CO2 absorption channels between 720 and 790 cm-1. The cloud microphysical properties can be retrieved by minimizing the differences between the observations and the calculations using AIRS longwave window channels between 790 and 1130 cm-1. AIRS is used to derive cloud properties during the Mixed Phase Arctic Cloud Experiment (MPACE) field campaign. Comparison with measurements obtained from lidar data is made for a test day, showing that AIRS cloud property retrievals agree with in situ lidar observations. Due to the large solar zenith angle, the MODIS operational retrieval approach is not able to provide cloud microphysics north of Barrow, Alaska; however, AIRS provides cloud microphysical properties with its high spectral resolution IR measurements.

Surface reflectance retrieval from imaging spectrometer data using three atmospheric codes

NASA Astrophysics Data System (ADS)

Staenz, Karl; Williams, Daniel J.; Fedosejevs, Gunar; Teillet, Phil M.

1994-12-01

Surface reflectance retrieval from imaging spectrometer data has become important for quantitative information extraction in many application areas. In order to calculate surface reflectance from remotely measured radiance, radiative transfer codes play an important role for removal of the scattering and gaseous absorption effects of the atmosphere. The present study evaluates surface reflectances retrieved from airborne visible/infrared imaging spectrometer (AVIRIS) data using three radiative transfer codes: modified 5S (M5S), 6S, and MODTRAN2. Comparisons of the retrieved surface reflectance with ground-based reflectance were made for different target types such as asphalt, gravel, grass/soil mixture (soccer field), and water (Sooke Lake). The results indicate that the estimation of the atmospheric water vapor content is important for an accurate surface reflectance retrieval regardless of the radiative transfer code used. For the present atmospheric conditions, a difference of 0.1 in aerosol optical depth had little impact on the retrieved surface reflectance. The performance of MODTRAN2 is superior in the gas absorption regions compared to M5S and 6S.

Optical information authentication using compressed double-random-phase-encoded images and quick-response codes.

PubMed

Wang, Xiaogang; Chen, Wen; Chen, Xudong

2015-03-09

In this paper, we develop a new optical information authentication system based on compressed double-random-phase-encoded images and quick-response (QR) codes, where the parameters of optical lightwave are used as keys for optical decryption and the QR code is a key for verification. An input image attached with QR code is first optically encoded in a simplified double random phase encoding (DRPE) scheme without using interferometric setup. From the single encoded intensity pattern recorded by a CCD camera, a compressed double-random-phase-encoded image, i.e., the sparse phase distribution used for optical decryption, is generated by using an iterative phase retrieval technique with QR code. We compare this technique to the other two methods proposed in literature, i.e., Fresnel domain information authentication based on the classical DRPE with holographic technique and information authentication based on DRPE and phase retrieval algorithm. Simulation results show that QR codes are effective on improving the security and data sparsity of optical information encryption and authentication system.

Effects of cue focality on the neural mechanisms of prospective memory: A meta-analysis of neuroimaging studies.

PubMed

Cona, Giorgia; Bisiacchi, Patrizia Silvia; Sartori, Giuseppe; Scarpazza, Cristina

2016-05-17

Remembering to execute pre-defined intentions at the appropriate time in the future is typically referred to as Prospective Memory (PM). Studies of PM showed that distinct cognitive processes underlie the execution of delayed intentions depending on whether the cue associated with such intentions is focal to ongoing activity processing or not (i.e., cue focality). The present activation likelihood estimation (ALE) meta-analysis revealed several differences in brain activity as a function of focality of the PM cue. The retrieval of intention is supported mainly by left anterior prefrontal cortex (Brodmann Area, BA 10) in nonfocal tasks, and by cerebellum and ventral parietal regions in focal tasks. Furthermore, the precuneus showed increased activation during the maintenance phase of intentions compared to the retrieval phase in nonfocal tasks, whereas the inferior parietal lobule showed increased activation during the retrieval of intention compared to maintenance phase in the focal tasks. Finally, the retrieval of intention relies more on the activity in anterior cingulate cortex for nonfocal tasks, and on posterior cingulate cortex for focal tasks. Such focality-related pattern of activations suggests that prospective remembering is mediated mainly by top-down and stimulus-independent processes in nonfocal tasks, whereas by more automatic, bottom-up, processes in focal tasks.

Effects of cue focality on the neural mechanisms of prospective memory: A meta-analysis of neuroimaging studies

PubMed Central

Cona, Giorgia; Bisiacchi, Patrizia Silvia; Sartori, Giuseppe; Scarpazza, Cristina

2016-01-01

Remembering to execute pre-defined intentions at the appropriate time in the future is typically referred to as Prospective Memory (PM). Studies of PM showed that distinct cognitive processes underlie the execution of delayed intentions depending on whether the cue associated with such intentions is focal to ongoing activity processing or not (i.e., cue focality). The present activation likelihood estimation (ALE) meta-analysis revealed several differences in brain activity as a function of focality of the PM cue. The retrieval of intention is supported mainly by left anterior prefrontal cortex (Brodmann Area, BA 10) in nonfocal tasks, and by cerebellum and ventral parietal regions in focal tasks. Furthermore, the precuneus showed increased activation during the maintenance phase of intentions compared to the retrieval phase in nonfocal tasks, whereas the inferior parietal lobule showed increased activation during the retrieval of intention compared to maintenance phase in the focal tasks. Finally, the retrieval of intention relies more on the activity in anterior cingulate cortex for nonfocal tasks, and on posterior cingulate cortex for focal tasks. Such focality-related pattern of activations suggests that prospective remembering is mediated mainly by top-down and stimulus-independent processes in nonfocal tasks, whereas by more automatic, bottom-up, processes in focal tasks. PMID:27185531

Robust information encryption diffractive-imaging-based scheme with special phase retrieval algorithm for a customized data container

NASA Astrophysics Data System (ADS)

Qin, Yi; Wang, Zhipeng; Wang, Hongjuan; Gong, Qiong; Zhou, Nanrun

2018-06-01

The diffractive-imaging-based encryption (DIBE) scheme has aroused wide interesting due to its compact architecture and low requirement of conditions. Nevertheless, the primary information can hardly be recovered exactly in the real applications when considering the speckle noise and potential occlusion imposed on the ciphertext. To deal with this issue, the customized data container (CDC) into DIBE is introduced and a new phase retrieval algorithm (PRA) for plaintext retrieval is proposed. The PRA, designed according to the peculiarity of the CDC, combines two key techniques from previous approaches, i.e., input-support-constraint and median-filtering. The proposed scheme can guarantee totally the reconstruction of the primary information despite heavy noise or occlusion and its effectiveness and feasibility have been demonstrated with simulation results.

Retrieving the hydrous minerals on Mars by sparse unmixing and the Hapke model using MRO/CRISM data

NASA Astrophysics Data System (ADS)

Lin, Honglei; Zhang, Xia

2017-05-01

The hydrous minerals on Mars preserve records of potential past aqueous activity. Quantitative information regarding mineralogical composition would enable a better understanding of the formation processes of these hydrous minerals, and provide unique insights into ancient habitable environments and the geological evolution of Mars. The Compact Reconnaissance Imaging Spectrometer for Mars (CRISM) has the advantage of both a high spatial and spectral resolution, which makes it suitable for the quantitative analysis of minerals on Mars. However, few studies have attempted to quantitatively retrieve the mineralogical composition of hydrous minerals on Mars using visible-infrared (VISIR) hyperspectral data due to their distribution characteristics (relatively low concentrations, located primarily in Noachian terrain, and unclear or unknown background minerals) and limitations of the spectral unmixing algorithms. In this study, we developed a modified sparse unmixing (MSU) method, combining the Hapke model with sparse unmixing. The MSU method considers the nonlinear mixed effects of minerals and avoids the difficulty of determining the spectra and number of endmembers from the image. The proposed method was tested successfully using laboratory mixture spectra and an Airborne Visible Infrared Imaging Spectrometer (AVIRIS) image of the Cuprite site (Nevada, USA). Then it was applied to CRISM hyperspectral images over Gale crater. Areas of hydrous mineral distribution were first identified by spectral features of water and hydroxyl absorption. The MSU method was performed on these areas, and the abundances were retrieved. The results indicated that the hydrous minerals consisted mostly of hydrous silicates, with abundances of up to 35%, as well as hydrous sulfates, with abundances ≤10%. Several main subclasses of hydrous minerals (e.g., Fe/Mg phyllosilicate, prehnite, and kieserite) were retrieved. Among these, Fe/Mg- phyllosilicate was the most abundant, with abundances ranging up to almost 30%, followed by prehnite and kieserite, with abundances lower than 15%. Our results are consistent with related research and in situ analyses of data from the rover Curiosity; thus, our method has the potential to be widely used for quantitative mineralogical mapping at the global scale of the surface of Mars.

An evaluation of three-dimensional sensors for the extravehicular activity helper/retreiver

NASA Technical Reports Server (NTRS)

Magee, Michael

1993-01-01

The Extravehicular Activity Retriever/Helper (EVAHR) is a robotic device currently under development at the NASA Johnson Space Center that is designed to fetch objects or to assist in retrieving an astronaut who may have become inadvertently de-tethered. The EVAHR will be required to exhibit a high degree of intelligent autonomous operation and will base much of its reasoning upon information obtained from one or more three-dimensional sensors that it will carry and control. At the highest level of visual cognition and reasoning, the EVAHR will be required to detect objects, recognize them, and estimate their spatial orientation and location. The recognition phase and estimation of spatial pose will depend on the ability of the vision system to reliably extract geometric features of the objects such as whether the surface topologies observed are planar or curved and the spatial relationships between the component surfaces. In order to achieve these tasks, accurate sensing of the operational environment and objects in the environment will therefore be critical. The qualitative and quantitative results of empirical studies of three sensors that are capable of providing three-dimensional information to the EVAHR, but using completely different hardware approaches are documented. The first of these devices is a phase shift laser with an effective operating range (ambiguity interval) of approximately 15 meters. The second sensor is a laser triangulation system designed to operate at much closer range and to provide higher resolution images. The third sensor is a dual camera stereo imaging system from which range images can also be obtained. The remainder of the report characterizes the strengths and weaknesses of each of these systems relative to quality of data extracted and how different object characteristics affect sensor operation.

NASA's GeneLab Phase II: Federated Search and Data Discovery

NASA Technical Reports Server (NTRS)

Berrios, Daniel C.; Costes, Sylvain V.; Tran, Peter B.

2017-01-01

GeneLab is currently being developed by NASA to accelerate 'open science' biomedical research in support of the human exploration of space and the improvement of life on earth. Phase I of the four-phase GeneLab Data Systems (GLDS) project emphasized capabilities for submission, curation, search, and retrieval of genomics, transcriptomics and proteomics ('omics') data from biomedical research of space environments. The focus of development of the GLDS for Phase II has been federated data search for and retrieval of these kinds of data across other open-access systems, so that users are able to conduct biological meta-investigations using data from a variety of sources. Such meta-investigations are key to corroborating findings from many kinds of assays and translating them into systems biology knowledge and, eventually, therapeutics.

NASAs GeneLab Phase II: Federated Search and Data Discovery

NASA Technical Reports Server (NTRS)

Berrios, Daniel C.; Costes, Sylvain; Tran, Peter

2017-01-01

GeneLab is currently being developed by NASA to accelerate open science biomedical research in support of the human exploration of space and the improvement of life on earth. Phase I of the four-phase GeneLab Data Systems (GLDS) project emphasized capabilities for submission, curation, search, and retrieval of genomics, transcriptomics and proteomics (omics) data from biomedical research of space environments. The focus of development of the GLDS for Phase II has been federated data search for and retrieval of these kinds of data across other open-access systems, so that users are able to conduct biological meta-investigations using data from a variety of sources. Such meta-investigations are key to corroborating findings from many kinds of assays and translating them into systems biology knowledge and, eventually, therapeutics.

Information verification and encryption based on phase retrieval with sparsity constraints and optical inference

NASA Astrophysics Data System (ADS)

Zhong, Shenlu; Li, Mengjiao; Tang, Xiajie; He, Weiqing; Wang, Xiaogang

2017-01-01

A novel optical information verification and encryption method is proposed based on inference principle and phase retrieval with sparsity constraints. In this method, a target image is encrypted into two phase-only masks (POMs), which comprise sparse phase data used for verification. Both of the two POMs need to be authenticated before being applied for decrypting. The target image can be optically reconstructed when the two authenticated POMs are Fourier transformed and convolved by the correct decryption key, which is also generated in encryption process. No holographic scheme is involved in the proposed optical verification and encryption system and there is also no problem of information disclosure in the two authenticable POMs. Numerical simulation results demonstrate the validity and good performance of this new proposed method.

A control approach for robots with flexible links and rigid end-effectors

NASA Technical Reports Server (NTRS)

Barbieri, Enrique; Ozguner, Umit

1989-01-01

Multiarm flexible robots with dexterous end effectors are currently being considered in such tasks as satellite retrieval, servicing and repair where a two phase problem can be identified: Phase 1, robot positioning in space; Phase 2, object retrieval. Some issues in Phase 1 regarding modelling and control strategies for a robotic system comprised of along flexible arm and a rigid three-link end effector are presented. The control objective is to maintain the last (rigid) link stationary in space in the presence of an additive disturbance caused by the flexible energy in the first link after a positioning maneuver has been accomplished. Several configuration strategies can be considered, and optimal decentralized servocompensators can be designed. Preliminary computer simulations are included for a simple proportional controller to illustrate the approach.

Frequency–specific network connectivity increases underlie accurate spatiotemporal memory retrieval

PubMed Central

Watrous, Andrew J.; Tandon, Nitin; Connor, Chris; Pieters, Thomas; Ekstrom, Arne D.

2013-01-01

The medial temporal lobes, prefrontal cortex, and parts of parietal cortex form the neural underpinnings of episodic memory, which includes remembering both where and when an event occurred. Yet how these three key regions interact during retrieval of spatial and temporal context remains largely untested. Here, we employed simultaneous electrocorticographical recordings across multiple lobular regions, employing phase synchronization as a measure of network functional connectivity, while patients retrieved spatial and temporal context associated with an episode. Successful memory retrieval was characterized by greater global connectivity compared to incorrect retrieval, with the MTL acting as a convergence hub for these interactions. Spatial vs. temporal context retrieval resulted in prominent differences in both the spectral and temporal patterns of network interactions. These results emphasize dynamic network interactions as central to episodic memory retrieval, providing novel insight into how multiple contexts underlying a single event can be recreated within the same network. PMID:23354333

Validation of Cloud Properties From Multiple Satellites Using CALIOP Data

NASA Technical Reports Server (NTRS)

Yost, Christopher R.; Minnis, Patrick; Bedka, Kristopher M.; Heck, Patrick W.; Palikonda, Rabindra; Sun-Mack, Sunny; Trepte, Qing

2016-01-01

The NASA Langley Satellite ClOud and Radiative Property retrieval System (SatCORPS) is routinely applied to multispectral imagery from several geostationary and polar-orbiting imagers to retrieve cloud properties for weather and climate applications. Validation of the retrievals with independent datasets is continuously ongoing in order to understand differences caused by calibration, spatial resolution, viewing geometry, and other factors. The CALIOP instrument provides a decade of detailed cloud observations which can be used to evaluate passive imager retrievals of cloud boundaries, thermodynamic phase, cloud optical depth, and water path on a global scale. This paper focuses on comparisons of CALIOP retrievals to retrievals from MODIS, VIIRS, AVHRR, GOES, SEVIRI, and MTSAT. CALIOP is particularly skilled at detecting weakly-scattering cirrus clouds with optical depths less than approx. 0.5. These clouds are often undetected by passive imagers and the effect this has on the property retrievals is discussed.

Development, Validation, and Potential Enhancements to the Second-Generation Operational Aerosol Product at the National Environmental Satellite, Data, and Information Service of the National Oceanic and Atmospheric Administration

NASA Technical Reports Server (NTRS)

Stowe, Larry L.; Ignatov, Alexander M.; Singh, Ramdas R.

1997-01-01

A revised (phase 2) single-channel algorithm for aerosol optical thickness, tau(sup A)(sub SAT), retrieval over oceans from radiances in channel 1 (0.63 microns) of the Advanced Very High Resolution Radiometer (AVHRR) has been implemented at the National Oceanic and Atmospheric Administration's National Environmental Satellite Data and Information Service for the NOAA 14 satellite launched December 30, 1994. It is based on careful validation of its operational predecessor (phase 1 algorithm), implemented for NOAA 14 in 1989. Both algorithms scale the upward satellite radiances in cloud-free conditions to aerosol optical thickness using an updated radiative transfer model of the ocean and atmosphere. Application of the phase 2 algorithm to three matchup Sun-photometer and satellite data sets, one with NOAA 9 in 1988 and two with NOAA 11 in 1989 and 1991, respectively, show systematic error is less than 10%, with a random error of sigma(sub tau) approx. equal 0.04. First results of tau(sup A)(sub SAT) retrievals from NOAA 14 using the phase 2 algorithm, and from checking its internal consistency, are presented. The potential two-channel (phase 3) algorithm for the retrieval of an aerosol size parameter, such as the Junge size distribution exponent, by adding either channel 2 (0.83 microns) from the current AVHRR instrument, or a 1.6-microns channel to be available on the Tropical Rainfall Measurement Mission and the NOAA-KLM satellites by 1997 is under investigation. The possibility of using this additional information in the retrieval of a more accurate estimate of aerosol optical thickness is being explored.

Role of dorsal hippocampal orexin-1 receptors in memory restoration induced by morphine sensitization phenomenon.

PubMed

Alijanpour, S; Tirgar, F; Zarrindast, M-R

2016-01-15

The present study was examined the blockade of CA1 orexin-1 receptors (OX1Rs) of the dorsal hippocampus in the induction or expression phase on morphine sensitization-induced memory restoration using the Morris water maze (MWM) apparatus. Results showed that pre-training administration of morphine (5mg/kg, s.c.) increases escape latency and traveled distance, while does not alter swimming speed. This supports the impairing effect of morphine on the spatial memory acquisition in male adult rats. Also, in the retrieval session (probe trial) this treatment decreased the time spent in the target quadrant. Moreover, morphine-induced sensitization (15 or 20mg/kg, s.c.; once daily for 3days and followed by 5days no drug treatment) restored the memory acquisition/retrieval deficit which had been induced by pre-training administration of morphine (5mg/kg, s.c.). Intra-CA1 microinjection of subthreshold doses of SB-334867 (OX1Rs antagonist; 10, 20 and 40nmol/rat), 5min before morphine (20mg/kg/day×3days, s.c.; induction phase for morphine sensitization) did not alter restoration of memory acquisition/retrieval produced by the morphine sensitization phenomenon. In contrast, microinjection of subthreshold doses of SB-334867 (10, 20 and 40nmol/rat) into the CA1 region in the training session, 5min prior to morphine (5mg/kg, s.c.; expression phase for morphine sensitization) blocked the spatial memory acquisition/retrieval in morphine-sensitized rats. In conclusion, these findings show that morphine sensitization reverses morphine-induced amnesia. Furthermore, the blockade of CA1 OX1Rs in the expression phase, but not in the induction phase, disrupts memory restoration induced by morphine sensitization. Copyright © 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

Secret shared multiple-image encryption based on row scanning compressive ghost imaging and phase retrieval in the Fresnel domain

NASA Astrophysics Data System (ADS)

Li, Xianye; Meng, Xiangfeng; Wang, Yurong; Yang, Xiulun; Yin, Yongkai; Peng, Xiang; He, Wenqi; Dong, Guoyan; Chen, Hongyi

2017-09-01

A multiple-image encryption method is proposed that is based on row scanning compressive ghost imaging, (t, n) threshold secret sharing, and phase retrieval in the Fresnel domain. In the encryption process, after wavelet transform and Arnold transform of the target image, the ciphertext matrix can be first detected using a bucket detector. Based on a (t, n) threshold secret sharing algorithm, the measurement key used in the row scanning compressive ghost imaging can be decomposed and shared into two pairs of sub-keys, which are then reconstructed using two phase-only mask (POM) keys with fixed pixel values, placed in the input plane and transform plane 2 of the phase retrieval scheme, respectively; and the other POM key in the transform plane 1 can be generated and updated by the iterative encoding of each plaintext image. In each iteration, the target image acts as the input amplitude constraint in the input plane. During decryption, each plaintext image possessing all the correct keys can be successfully decrypted by measurement key regeneration, compression algorithm reconstruction, inverse wavelet transformation, and Fresnel transformation. Theoretical analysis and numerical simulations both verify the feasibility of the proposed method.

The fate of completed intentions.

PubMed

Anderson, Francis T; Einstein, Gilles O

2017-04-01

The goal of this research was to determine whether and how people deactivate prospective memory (PM) intentions after they have been completed. One view proposes that PM intentions can be deactivated after completion, such that they no longer come to mind and interfere with current tasks. Another view is that now irrelevant completed PM intentions exhibit persisting activation, and continue to be retrieved. In Experiment 1, participants were given a PM intention embedded within the ongoing task during Phase 1, after which participants were told either that the PM task had been completed or suspended until later. During Phase 2, participants were instructed to perform only the ongoing task and were periodically prompted to report their thoughts. Critically, the PM targets from Phase 1 reappeared in Phase 2. All of our measures, including thoughts reported about the PM task, supported the existence of persisting activation. In Experiment 2, we varied conditions that were expected to mitigate persisting activation. Despite our best attempts to promote deactivation, we found evidence for the persistence of spontaneous retrieval in all groups after intentions were completed. The theoretical and practical implications of this potential dark side to spontaneous retrieval are discussed.

Dreaming of Atmospheres

NASA Astrophysics Data System (ADS)

Waldmann, I. P.

2016-04-01

Here, we introduce the RobERt (Robotic Exoplanet Recognition) algorithm for the classification of exoplanetary emission spectra. Spectral retrieval of exoplanetary atmospheres frequently requires the preselection of molecular/atomic opacities to be defined by the user. In the era of open-source, automated, and self-sufficient retrieval algorithms, manual input should be avoided. User dependent input could, in worst-case scenarios, lead to incomplete models and biases in the retrieval. The RobERt algorithm is based on deep-belief neural (DBN) networks trained to accurately recognize molecular signatures for a wide range of planets, atmospheric thermal profiles, and compositions. Reconstructions of the learned features, also referred to as the “dreams” of the network, indicate good convergence and an accurate representation of molecular features in the DBN. Using these deep neural networks, we work toward retrieval algorithms that themselves understand the nature of the observed spectra, are able to learn from current and past data, and make sensible qualitative preselections of atmospheric opacities to be used for the quantitative stage of the retrieval process.

Uncertainty in cloud optical depth estimates made from satellite radiance measurements

NASA Technical Reports Server (NTRS)

Pincus, Robert; Szczodrak, Malgorzata; Gu, Jiujing; Austin, Philip

1995-01-01

The uncertainty in optical depths retrieved from satellite measurements of visible wavelength radiance at the top of the atmosphere is quantified. Techniques are briefly reviewed for the estimation of optical depth from measurements of radiance, and it is noted that these estimates are always more uncertain at greater optical depths and larger solar zenith angles. The lack of radiometric calibration for visible wavelength imagers on operational satellites dominates the uncertainty retrievals of optical depth. This is true for both single-pixel retrievals and for statistics calculated from a population of individual retrievals. For individual estimates or small samples, sensor discretization can also be significant, but the sensitivity of the retrieval to the specification of the model atmosphere is less important. The relative uncertainty in calibration affects the accuracy with which optical depth distributions measured by different sensors may be quantitatively compared, while the absolute calibration uncertainty, acting through the nonlinear mapping of radiance to optical depth, limits the degree to which distributions measured by the same sensor may be distinguished.

Coherent diffractive imaging using randomly coded masks

DOE PAGES

Seaberg, Matthew H.; d'Aspremont, Alexandre; Turner, Joshua J.

2015-12-07

We experimentally demonstrate an extension to coherent diffractive imaging that encodes additional information through the use of a series of randomly coded masks, removing the need for typical object-domain constraints while guaranteeing a unique solution to the phase retrieval problem. Phase retrieval is performed using a numerical convex relaxation routine known as “PhaseCut,” an iterative algorithm known for its stability and for its ability to find the global solution, which can be found efficiently and which is robust to noise. As a result, the experiment is performed using a laser diode at 532.2 nm, enabling rapid prototyping for future X-raymore » synchrotron and even free electron laser experiments.« less

Coherent diffractive imaging using randomly coded masks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Seaberg, Matthew H., E-mail: seaberg@slac.stanford.edu; Linac Coherent Light Source, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025; D'Aspremont, Alexandre

2015-12-07

We experimentally demonstrate an extension to coherent diffractive imaging that encodes additional information through the use of a series of randomly coded masks, removing the need for typical object-domain constraints while guaranteeing a unique solution to the phase retrieval problem. Phase retrieval is performed using a numerical convex relaxation routine known as “PhaseCut,” an iterative algorithm known for its stability and for its ability to find the global solution, which can be found efficiently and which is robust to noise. The experiment is performed using a laser diode at 532.2 nm, enabling rapid prototyping for future X-ray synchrotron and even freemore » electron laser experiments.« less

Phase retrieval in digital speckle pattern interferometry by use of a smoothed space-frequency distribution.

PubMed

Federico, Alejandro; Kaufmann, Guillermo H

2003-12-10

We evaluate the use of a smoothed space-frequency distribution (SSFD) to retrieve optical phase maps in digital speckle pattern interferometry (DSPI). The performance of this method is tested by use of computer-simulated DSPI fringes. Phase gradients are found along a pixel path from a single DSPI image, and the phase map is finally determined by integration. This technique does not need the application of a phase unwrapping algorithm or the introduction of carrier fringes in the interferometer. It is shown that a Wigner-Ville distribution with a smoothing Gaussian kernel gives more-accurate results than methods based on the continuous wavelet transform. We also discuss the influence of filtering on smoothing of the DSPI fringes and some additional limitations that emerge when this technique is applied. The performance of the SSFD method for processing experimental data is then illustrated.

An evaluation of information retrieval accuracy with simulated OCR output

DOE Office of Scientific and Technical Information (OSTI.GOV)

Croft, W.B.; Harding, S.M.; Taghva, K.

Optical Character Recognition (OCR) is a critical part of many text-based applications. Although some commercial systems use the output from OCR devices to index documents without editing, there is very little quantitative data on the impact of OCR errors on the accuracy of a text retrieval system. Because of the difficulty of constructing test collections to obtain this data, we have carried out evaluation using simulated OCR output on a variety of databases. The results show that high quality OCR devices have little effect on the accuracy of retrieval, but low quality devices used with databases of short documents canmore » result in significant degradation.« less

General quantitative analysis of stress partitioning and boundary conditions in undrained biphasic porous media via a purely macroscopic and purely variational approach

NASA Astrophysics Data System (ADS)

Serpieri, Roberto; Travascio, Francesco

2016-03-01

In poroelasticity, the effective stress law relates the external stress applied to the medium to the macroscopic strain of the solid phase and the interstitial pressure of the fluid saturating the mixture. Such relationship has been formerly introduced by Terzaghi in form of a principle. To date, no poroelastic theory is capable of recovering a stress partitioning law in agreement with Terzaghi's postulated one in the absence of ad hoc constitutive assumptions on the medium. We recently proposed a variational macroscopic continuum description of two-phase poroelasticity to derive a general biphasic formulation at finite deformations, termed variational macroscopic theory of porous media (VMTPM). Such approach proceeds from the inclusion of the intrinsic volumetric strain among the kinematic descriptors aside to macroscopic displacements, and as a variational theory, uses the Hamilton least-action principle as the unique primitive concept of mechanics invoked to derive momentum balance equations. In a previous related work it was shown that, for the subclass of undrained problems, VMTPM predicts that stress is partitioned in the two phases in strict compliance with Terzaghi's law, irrespective of the microstructural and constitutive features of a given medium. In the present contribution, we further develop the linearized framework of VMTPM to arrive at a general operative formula that allows the quantitative determination of stress partitioning in a jacketed test over a generic isotropic biphasic specimen. This formula is quantitative and general, in that it relates the partial phase stresses to the externally applied stress as function of partitioning coefficients that are all derived by strictly following a purely variational and purely macroscopic approach, and in the absence of any specific hypothesis on the microstructural or constitutive features of a given medium. To achieve this result, the stiffness coefficients of the theory are derived by using exclusively variational arguments. We derive the boundary conditions attained across the boundary of a poroelastic saturated medium in contact with an impermeable surface also based on purely variational arguments. A technique to retrieve bounds for the resulting elastic moduli, based on Hashin's composite spheres assemblage method, is also reported. Notably, in spite of the minimal mechanical hypotheses introduced, a rich mechanical behavior is observed.

Retrieval of Droplet size Density Distribution from Multiple field of view Cross polarized Lidar Signals: Theory and Experimental Validation

DTIC Science & Technology

2016-06-02

Retrieval of droplet-size density distribution from multiple-field-of-view cross-polarized lidar signals: theory and experimental validation...theoretical and experimental studies of mul- tiple scattering and multiple-field-of-view (MFOV) li- dar detection have made possible the retrieval of cloud...droplet cloud are typical of Rayleigh scattering, with a signature close to a dipole (phase function quasi -flat and a zero-depolarization ratio

Retrieving both phase and amplitude information of Green's functions by ambient seismic wave field cross-correlation: A case study with a limestone mine induced seismic event

NASA Astrophysics Data System (ADS)

Kwak, S.; Song, S. G.; Kim, G.; Shin, J. S.

2015-12-01

Recently many seismologists have paid attention to ambient seismic field, which is no more referred as noise and called as Earth's hum, but as useful signal to understand subsurface seismic velocity structure. It has also been demonstrated that empirical Green's functions can be constructed by retrieving both phase and amplitude information from ambient seismic field (Prieto and Beroza 2008). The constructed empirical Green's functions can be used to predict strong ground motions after focal depth and double-couple mechanism corrections (Denolle et al. 2013). They do not require detailed subsurface velocity model and intensive computation for ground motion simulation. In this study, we investigate the capability of predicting long period surface waves by the ambient seismic wave field with a seismic event of Mw 4.0, which occurred with a limestone mine collapse in South Korea on January 31, 2015. This limestone-mine event provides an excellent opportunity to test the efficiency of the ambient seismic wave field in retrieving both phase and amplitude information of Green's functions due to the single force mechanism of the collapse event. In other words, both focal depth and double-couple mechanism corrections are not required for this event. A broadband seismic station, which is about 5.4 km away from the mine event, is selected as a source station. Then surface waves retrieved from the ambient seismic wave field cross-correlation are compared with those generated by the event. Our preliminary results show some potential of the ambient seismic wave field in retrieving both phase and amplitude of Green's functions from a single force impulse source at the Earth's surface. More comprehensive analysis by increasing the time length of stacking may improve the results in further studies. We also aim to investigate the efficiency of retrieving the full empirical Green's functions with the 2007 Mw 4.6 Odaesan earthquake, which is one of the strongest earthquakes occurred in South Korea in the last decade.

The Negative Testing Effect and Multifactor Account

ERIC Educational Resources Information Center

Peterson, Daniel J.; Mulligan, Neil W.

2013-01-01

Across 3 experiments, we investigated the factors that dictate when taking a test improves subsequent memory performance (the "testing effect"). In Experiment 1, participants retrieving a set of targets during a retrieval practice phase ultimately recalled fewer of those targets compared with a group of participants who studied the…

Initial retrieval shields against retrieval-induced forgetting

PubMed Central

Racsmány, Mihály; Keresztes, Attila

2015-01-01

Testing, as a form of retrieval, can enhance learning but it can also induce forgetting of related memories, a phenomenon known as retrieval-induced forgetting (RIF). In four experiments we explored whether selective retrieval and selective restudy of target memories induce forgetting of related memories with or without initial retrieval of the entire learning set. In Experiment 1, subjects studied category-exemplar associations, some of which were then either restudied or retrieved. RIF occurred on a delayed final test only when memories were retrieved and not when they were restudied. In Experiment 2, following the study phase of category-exemplar associations, subjects attempted to recall all category-exemplar associations, then they selectively retrieved or restudied some of the exemplars. We found that, despite the huge impact on practiced items, selective retrieval/restudy caused no decrease in final recall of related items. In Experiment 3, we replicated the main result of Experiment 2 by manipulating initial retrieval as a within-subject variable. In Experiment 4 we replicated the main results of the previous experiments with non-practiced (Nrp) baseline items. These findings suggest that initial retrieval of the learning set shields against the forgetting effect of later selective retrieval. Together, our results support the context shift theory of RIF. PMID:26052293

Retrieving the Polar Mixed-Phase Cloud Liquid Water Path by Combining CALIOP and IIR Measurements

NASA Astrophysics Data System (ADS)

Luo, Tao; Wang, Zhien; Li, Xuebin; Deng, Shumei; Huang, Yong; Wang, Yingjian

2018-02-01

Mixed-phase cloud (MC) is the dominant cloud type over the polar region, and there are challenging conditions for remote sensing and in situ measurements. In this study, a new methodology of retrieving the stratiform MC liquid water path (LWP) by combining Cloud-Aerosol Lidar with Orthogonal Polarization (CALIOP) and infrared imaging radiometer (IIR) measurements was developed and evaluated. This new methodology takes the advantage of reliable cloud-phase discrimination by combining lidar and radar measurements. An improved multiple-scattering effect correction method for lidar signals was implemented to provide reliable cloud extinction near cloud top. Then with the adiabatic cloud assumption, the MC LWP can be retrieved by a lookup-table-based method. Simulations with error-free inputs showed that the mean bias and the root mean squared error of the LWP derived from the new method are -0.23 ± 2.63 g/m2, with the mean absolute relative error of 4%. Simulations with erroneous inputs suggested that the new methodology could provide reliable retrieval of LWP to support the statistical or climatology analysis. Two-month A-train satellite retrievals over Arctic region showed that the new method can produce very similar cloud top temperature (CTT) dependence of LWP to the ground-based microwave radiometer measurements, with a bias of -0.78 g/m2 and a correlation coefficient of 0.95 between the two mean CTT-LWP relationships. The new approach can also produce reasonable pattern and value of LWP in spatial distribution over the Arctic region.

Quantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast

PubMed Central

Lu, Hangwen; Chung, Jaebum; Ou, Xiaoze; Yang, Changhuei

2016-01-01

Differential phase contrast (DPC) is a non-interferometric quantitative phase imaging method achieved by using an asymmetric imaging procedure. We report a pupil modulation differential phase contrast (PMDPC) imaging method by filtering a sample’s Fourier domain with half-circle pupils. A phase gradient image is captured with each half-circle pupil, and a quantitative high resolution phase image is obtained after a deconvolution process with a minimum of two phase gradient images. Here, we introduce PMDPC quantitative phase image reconstruction algorithm and realize it experimentally in a 4f system with an SLM placed at the pupil plane. In our current experimental setup with the numerical aperture of 0.36, we obtain a quantitative phase image with a resolution of 1.73μm after computationally removing system aberrations and refocusing. We also extend the depth of field digitally by 20 times to ±50μm with a resolution of 1.76μm. PMID:27828473

Tomographic reconstruction of storm time RC ion distribution from ENA images on board multiple spacecraft

NASA Astrophysics Data System (ADS)

Ma, Shu-Ying; Yan, Wei-Nan; Xu, Liang

2015-11-01

A quantitative retrieval of 3-D distribution of energetic ions as energetic neutral atoms (ENA) sources is a challenging task. In this paper the voxel computerized tomography (CT) method is initially applied to reconstruct the 3-D distribution of energetic ions in the magnetospheric ring current (RC) region from ENA emission images on board multiple spacecraft. To weaken the influence of low-altitude emission (LAE) on the reconstruction, the LAE-associated ENA intensities are corrected by invoking the thick-target approximation. To overcome the divergence in iteration due to discordant instrument biases, a differential ENA voxel CT method is developed. The method is proved reliable and advantageous by numerical simulation for the case of constant bias independent of viewing angle. Then this method is implemented with ENA data measured by the Two Wide-angle Imaging Neutral-atom Spectrometers mission which performs stereoscopic ENA imaging. The 3-D spatial distributions and energy spectra of RC ion flux intensity are reconstructed for energies of 4-50 keV during the main phase of a major magnetic storm. The retrieved ion flux distributions seem to correspond to an asymmetric partial RC, located mainly around midnight favoring the postmidnight with L = 3.5-7.0 in the equatorial plane. The RC ion distributions with magnetic local time depend on energy, with major equatorial flux peak for lower energy located east of that for higher energy. In comparison with the ion energy spectra measured by Time History of Events and Macroscale Interactions during Substorms-D satellite flying in the RC region, the retrieved spectrum from remotely sensed ENA images are well matched with the in situ measurements.

Serial killers, spiders and cybersex: Social and survival information bias in the transmission of urban legends.

PubMed

Stubbersfield, Joseph M; Tehrani, Jamshid J; Flynn, Emma G

2015-05-01

This study uses urban legends to examine the effects of the social information bias and survival information bias on cultural transmission across three phases of transmission: the choose-to-receive phase, the encode-and-retrieve phase, and the choose-to-transmit phase. In line with previous research into content biases, a linear transmission chain design with 60 participants aged 18-52 was used to examine the encode-and-retrieve phase, while participants were asked to rank their interest in reading the story behind a headline and passing a story on for the other two phases. Legends which contained social information (Social Type), legends which contained survival information (Survival Type), and legends which contained both forms of information (Combined Type) were all recalled with significantly greater accuracy than control material, while Social and Combined Type legends were recalled with significantly greater accuracy than Survival Type legends. In another study with 30 participants aged 18-22, no significant differences were found between legend types in either the choose-to-receive phase or the choose-to-transmit phase. © 2014 The British Psychological Society.

Retrieval of the thickness and refractive index dispersion of parallel plate from a single interferogram recorded in both spectral and angular domains

NASA Astrophysics Data System (ADS)

Dong, Jingtao; Lu, Rongsheng

2018-04-01

The principle of retrieving the thickness and refractive index dispersion of a parallel glass plate is reported based on single interferogram recording and phase analysis. With the parallel plate illuminated by a convergent light sheet, the transmitted light interfering in both spectral and angular domains is recorded. The phase recovered from the single interferogram by Fourier analysis is used to retrieve the thickness and refractive index dispersion without periodic ambiguity. Experimental results of an optical substrate standard show that the accuracy of refractive index dispersion is less than 2.5 × 10-5 and the relative uncertainty of thickness is 6 × 10-5 (3σ). This method is confirmed to be robust against the intensity noises, indicating the capability of stable and accurate measurement.

The MODIS Aerosol Algorithm, Products and Validation

NASA Technical Reports Server (NTRS)

Remer, L. A.; Kaufman, Y. J.; Tanre, D.; Mattoo, S.; Chu, D. A.; Martins, J. V.; Li, R.-R.; Ichoku, C.; Levy, R. C.; Kleidman, R. G.

2003-01-01

The MODerate resolution Imaging Spectroradiometer (MODIS) aboard both NASA's Terra and Aqua satellites is making near global daily observations of the earth in a wide spectral range. These measurements are used to derive spectral aerosol optical thickness and aerosol size parameters over both land and ocean. The aerosol products available over land include aerosol optical thickness at three visible wavelengths, a measure of the fraction of aerosol optical thickness attributed to the fine mode and several derived parameters including reflected spectral solar flux at top of atmosphere. Over ocean, the aerosol optical thickness is provided in seven wavelengths from 0.47 microns to 2.13 microns. In addition, quantitative aerosol size information includes effective radius of the aerosol and quantitative fraction of optical thickness attributed to the fine mode. Spectral aerosol flux, mass concentration and number of cloud condensation nuclei round out the list of available aerosol products over the ocean. The spectral optical thickness and effective radius of the aerosol over the ocean are validated by comparison with two years of AERONET data gleaned from 133 AERONET stations. 8000 MODIS aerosol retrievals colocated with AERONET measurements confirm that one-standard deviation of MODIS optical thickness retrievals fall within the predicted uncertainty of delta tauapproximately equal to plus or minus 0.03 plus or minus 0.05 tau over ocean and delta tay equal to plus or minus 0.05 plus or minus 0.15 tau over land. 271 MODIS aerosol retrievals co-located with AERONET inversions at island and coastal sites suggest that one-standard deviation of MODIS effective radius retrievals falls within delta r_eff approximately equal to 0.11 microns. The accuracy of the MODIS retrievals suggests that the product can be used to help narrow the uncertainties associated with aerosol radiative forcing of global climate.

Single shot multi-wavelength phase retrieval with coherent modulation imaging.

PubMed

Dong, Xue; Pan, Xingchen; Liu, Cheng; Zhu, Jianqiang

2018-04-15

A single shot multi-wavelength phase retrieval method is proposed by combining common coherent modulation imaging (CMI) and a low rank mixed-state algorithm together. A radiation beam consisting of multi-wavelength is illuminated on the sample to be observed, and the exiting field is incident on a random phase plate to form speckle patterns, which is the incoherent superposition of diffraction patterns of each wavelength. The exiting complex amplitude of the sample including both the modulus and phase of each wavelength can be reconstructed simultaneously from the recorded diffraction intensity using a low rank mixed-state algorithm. The feasibility of this proposed method was verified with visible light experimentally. This proposed method not only makes CMI realizable with partially coherent illumination but also can extend its application to various traditionally unrelated fields, where several wavelengths should be considered simultaneously.

Downscaling Thermal Infrared Radiance for Subpixel Land Surface Temperature Retrieval

PubMed Central

Liu, Desheng; Pu, Ruiliang

2008-01-01

Land surface temperature (LST) retrieved from satellite thermal sensors often consists of mixed temperature components. Retrieving subpixel LST is therefore needed in various environmental and ecological studies. In this paper, we developed two methods for downscaling coarse resolution thermal infrared (TIR) radiance for the purpose of subpixel temperature retrieval. The first method was developed on the basis of a scale-invariant physical model on TIR radiance. The second method was based on a statistical relationship between TIR radiance and land cover fraction at high spatial resolution. The two methods were applied to downscale simulated 990-m ASTER TIR data to 90-m resolution. When validated against the original 90-m ASTER TIR data, the results revealed that both downscaling methods were successful in capturing the general patterns of the original data and resolving considerable spatial details. Further quantitative assessments indicated a strong agreement between the true values and the estimated values by both methods. PMID:27879844

Downscaling Thermal Infrared Radiance for Subpixel Land Surface Temperature Retrieval.

PubMed

Liu, Desheng; Pu, Ruiliang

2008-04-06

Land surface temperature (LST) retrieved from satellite thermal sensors often consists of mixed temperature components. Retrieving subpixel LST is therefore needed in various environmental and ecological studies. In this paper, we developed two methods for downscaling coarse resolution thermal infrared (TIR) radiance for the purpose of subpixel temperature retrieval. The first method was developed on the basis of a scale-invariant physical model on TIR radiance. The second method was based on a statistical relationship between TIR radiance and land cover fraction at high spatial resolution. The two methods were applied to downscale simulated 990-m ASTER TIR data to 90-m resolution. When validated against the original 90-m ASTER TIR data, the results revealed that both downscaling methods were successful in capturing the general patterns of the original data and resolving considerable spatial details. Further quantitative assessments indicated a strong agreement between the true values and the estimated values by both methods.

A Novel Image Retrieval Based on Visual Words Integration of SIFT and SURF

PubMed Central

Ali, Nouman; Bajwa, Khalid Bashir; Sablatnig, Robert; Chatzichristofis, Savvas A.; Iqbal, Zeshan; Rashid, Muhammad; Habib, Hafiz Adnan

2016-01-01

With the recent evolution of technology, the number of image archives has increased exponentially. In Content-Based Image Retrieval (CBIR), high-level visual information is represented in the form of low-level features. The semantic gap between the low-level features and the high-level image concepts is an open research problem. In this paper, we present a novel visual words integration of Scale Invariant Feature Transform (SIFT) and Speeded-Up Robust Features (SURF). The two local features representations are selected for image retrieval because SIFT is more robust to the change in scale and rotation, while SURF is robust to changes in illumination. The visual words integration of SIFT and SURF adds the robustness of both features to image retrieval. The qualitative and quantitative comparisons conducted on Corel-1000, Corel-1500, Corel-2000, Oliva and Torralba and Ground Truth image benchmarks demonstrate the effectiveness of the proposed visual words integration. PMID:27315101

Retrieval, Monitoring, and Control Processes: A 7 Tesla fMRI Approach to Memory Accuracy

PubMed Central

Risius, Uda-Mareke; Staniloiu, Angelica; Piefke, Martina; Maderwald, Stefan; Schulte, Frank P.; Brand, Matthias; Markowitsch, Hans J.

2012-01-01

Memory research has been guided by two powerful metaphors: the storehouse (computer) and the correspondence metaphor. The latter emphasizes the dependability of retrieved mnemonic information and draws upon ideas about the state dependency and reconstructive character of episodic memory. We used a new movie to unveil the neural correlates connected with retrieval, monitoring, and control processes, and memory accuracy (MAC), according to the paradigm of Koriat and Goldsmith (1996a,b). During functional magnetic resonance imaging, subjects performed a memory task which required (after an initial learning phase) rating true and false statements [retrieval phase (RP)], making confidence judgments in the respective statement [monitoring phase (MP)], and deciding for either venturing (volunteering) the respective answer or withholding the response [control phase (CP)]. Imaging data pointed to common and unique neural correlates. Activations in brain regions related to RP and MAC were observed in the precuneus, middle temporal gyrus, and left hippocampus. MP was associated with activation in the left anterior and posterior cingulate cortex along with bilateral medial temporal regions. If an answer was volunteered (as opposed to being withheld) during the CP, temporal, and frontal as well as middle and posterior cingulate areas and the precuneus revealed activations. Increased bilateral hippocampal activity was found during withholding compared to volunteering answers. The left caudate activation detected during withholding compared to venturing an answer supports the involvement of the left caudate in inhibiting unwanted responses. Contrary to expectations, we did not evidence prefrontal activations during withholding (as opposed to volunteering) answers. This may reflect our design specifications, but alternative interpretations are put forth. PMID:23580061

Comparison of propagation-based phase-contrast tomography approaches for the evaluation of dentin microstructure

NASA Astrophysics Data System (ADS)

Deyhle, Hans; Weitkamp, Timm; Lang, Sabrina; Schulz, Georg; Rack, Alexander; Zanette, Irene; Müller, Bert

2012-10-01

The complex hierarchical structure of human tooth hard tissues, enamel and dentin, guarantees function for decades. On the micrometer level the dentin morphology is dominated by the tubules, micrometer-narrow channels extending from the dentin-enamel junction to the pulp chamber. Their structure has been extensively studied, mainly with two-dimensional approaches. Dentin tubules are formed during tooth growth and their orientation is linked to the morphology of the nanometer-sized components, which is of interest for example for the development of bio-inspired dental fillings. Therefore, a method has to be identified that can access the three-dimensional organization of the tubules, e.g. density and orientation. Tomographic setups with pixel sizes in the sub-micrometer range allow for the three-dimensional visualization of tooth dentin tubules both in phase and absorption contrast modes. We compare high-resolution tomographic scans reconstructed with propagation based phase retrieval algorithms as well as reconstructions without phase retrieval concerning spatial and density resolution as well as rendering of the dentin microstructure to determine the approach best suited for dentin tubule imaging. Reasonable results were obtained with a single-distance phase retrieval algorithm and a propagation distance of about 75% of the critical distance of d2/λ, where d is the size of the smallest objects identifiable in the specimen and λ is the X-ray wavelength.

COMPARISON OF GENETIC METHODS TO OPTICAL METHODS IN THE IDENTIFICATION AND ASSESSMENT OF MOLD IN THE BUILT ENVIRONMENT -- COMPARISON OF TAQMAN AND MICROSCOPIC ANALYSIS OF CLADOSPORIUM SPORES RETRIEVED FROM ZEFON AIR-O-CELL TRACES

EPA Science Inventory

Recent advances in the sequencing of relevant water intrusion fungi by the EPA, combined with the development of probes and primers have allowed for the unequivocal quantitative and qualitative identification of fungi in selected matrices.

In this pilot study, quantitative...

Inhibition of the mesoamygdala dopaminergic pathway impairs the retrieval of conditioned fear associations.

PubMed

Nader, K; LeDoux, J E

1999-10-01

Previous findings have demonstrated that systemic dopaminergic manipulations impair the retrieval of Pavlovian conditioned fear. A second-order fear-conditioning paradigm was used to test whether the dopaminergic projection from the ventral tegmental area (VTA) to the lateral and basal amygdala (LBA) can affect conditioned fear. Phase 1 entailed conditioned stimulus-unconditioned stimulus (CS1-US) pairings. In Phase 2, drugs were infused in either the LBA or VTA prior to pairings of CS2 (a second cue) with CS1. In Phase 3, freezing behavior elicited by CS2 was tested without drugs. Infusions of the D2 agonist quinpirole into the VTA or of the D1 antagonist SCH 23390 into the LBA caused a decrease in freezing to CS2. Both manipulations decrease D1 receptor activation in the LBA. Infusions of the D1 agonist SKF 38393 into the LBA had no effect. This pattern of results is consistent with the hypothesis that the VTA-LBA dopaminergic projection modulates the retrieval of an association between a CS and footshock US.

Phase retrieval and 3D imaging in gold nanoparticles based fluorescence microscopy (Conference Presentation)

NASA Astrophysics Data System (ADS)

Ilovitsh, Tali; Ilovitsh, Asaf; Weiss, Aryeh M.; Meir, Rinat; Zalevsky, Zeev

2017-02-01

Optical sectioning microscopy can provide highly detailed three dimensional (3D) images of biological samples. However, it requires acquisition of many images per volume, and is therefore time consuming, and may not be suitable for live cell 3D imaging. We propose the use of the modified Gerchberg-Saxton phase retrieval algorithm to enable full 3D imaging of gold nanoparticles tagged sample using only two images. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. The proposed concept is then further enhanced also for tracking of single fluorescent particles within a three dimensional (3D) cellular environment based on image processing algorithms that can significantly increases localization accuracy of the 3D point spread function in respect to regular Gaussian fitting. All proposed concepts are validated both on simulated data as well as experimentally.

Left Posterior Parietal Cortex Participates in Both Task Preparation and Episodic Retrieval

PubMed Central

Phillips, Jeffrey S.; Velanova, Katerina; Wolk, David A.; Wheeler, Mark E.

2012-01-01

Optimal memory retrieval depends not only on the fidelity of stored information, but also on the attentional state of the subject. Factors such as mental preparedness to engage in stimulus processing can facilitate or hinder memory retrieval. The current study used functional magnetic resonance imaging (fMRI) to distinguish preparatory brain activity before episodic and semantic retrieval tasks from activity associated with retrieval itself. A catch-trial imaging paradigm permitted separation of neural responses to preparatory task cues and memory probes. Episodic and semantic task preparation engaged a common set of brain regions, including the bilateral intraparietal sulcus (IPS), left fusiform gyrus (FG), and the pre-supplementary motor area (pre-SMA). In the subsequent retrieval phase, the left IPS was among a set of frontoparietal regions that responded differently to old and new stimuli. In contrast, the right IPS responded to preparatory cues with little modulation during memory retrieval. The findings support a strong left-lateralization of retrieval success effects in left parietal cortex, and further indicate that left IPS performs operations that are common to both task preparation and memory retrieval. Such operations may be related to attentional control, monitoring of stimulus relevance, or retrieval. PMID:19285142

Interruption of the Tower of London Task: Support for a Goal-Activation Approach

ERIC Educational Resources Information Center

Hodgetts, Helen M.; Jones, Dylan M.

2006-01-01

Unexpected interruptions introduced during the execution phase of simple Tower of London problems incurred a time cost when the interrupted goal was retrieved, and this cost was exacerbated the longer the goal was suspended. Furthermore, time taken to retrieve goals was greater following a more complex interruption, indicating the processing…

Encoding and Retrieval During Bimanual Rhythmic Coordination

ERIC Educational Resources Information Center

Shockley, Kevin; Turvey, Michael T.

2005-01-01

In 2 experiments, bimanual 1:1 rhythmic coordination was performed concurrently with encoding or retrieval of word lists. Effects of divided attention (DA) on coordination were indexed by changes in mean relative phase and recurrence measures of shared activity between the 2 limbs. Effects of DA on memory were indexed by deficits in recall…

Retrieval of concrete words involves more contextual information than abstract words: multiple components for the concreteness effect.

PubMed

Xiao, Xin; Zhao, Di; Zhang, Qin; Guo, Chun-yan

2012-03-01

The current study used the directed forgetting paradigm in implicit and explicit memory to investigate the concreteness effect. Event-related potentials (ERPs) were recorded to explore the neural basis of this phenomenon. The behavioral results showed a clear concreteness effect in both implicit and explicit memory tests; participants responded significantly faster to concrete words than to abstract words. The ERP results revealed a concreteness effect (N400) in both the encoding and retrieval phases. In addition, behavioral and ERP results showed an interaction between word concreteness and memory instruction (to-be-forgotten vs. to-be-remembered) in the late epoch of the explicit retrieval phase, revealing a significant concreteness effect only under the to-be-remembered instruction condition. This concreteness effect was realized as an increased P600-like component in response to concrete words relative to abstract words, likely reflecting retrieval of contextual details. The time course of the concreteness effect suggests advantages of concrete words over abstract words due to greater contextual information. Copyright © 2011 Elsevier Inc. All rights reserved.

Terahertz imaging with compressed sensing and phase retrieval.

PubMed

Chan, Wai Lam; Moravec, Matthew L; Baraniuk, Richard G; Mittleman, Daniel M

2008-05-01

We describe a novel, high-speed pulsed terahertz (THz) Fourier imaging system based on compressed sensing (CS), a new signal processing theory, which allows image reconstruction with fewer samples than traditionally required. Using CS, we successfully reconstruct a 64 x 64 image of an object with pixel size 1.4 mm using a randomly chosen subset of the 4096 pixels, which defines the image in the Fourier plane, and observe improved reconstruction quality when we apply phase correction. For our chosen image, only about 12% of the pixels are required for reassembling the image. In combination with phase retrieval, our system has the capability to reconstruct images with only a small subset of Fourier amplitude measurements and thus has potential application in THz imaging with cw sources.

Retrieval Practice and Spacing Effects in Young and Older Adults: An Examination of the Benefits of Desirable Difficulty

PubMed Central

Maddox, Geoffrey B.; Balota, David A.

2015-01-01

The present study examined how the function relating continued retrieval practice (e.g., 1, 3, or 5 tests) and long-term memory retention is modulated by desirable difficulty (Bjork, 1994). Of particular interest was how retrieval difficulty differed across young and older adults and across manipulations of lag (Experiment 1) and spacing (Experiment 2). To extend on previous studies, acquisition phase response latency was used as a proxy for retrieval difficulty, and analysis of final test performance was conditionalized on acquisition phase retrieval success to more directly examine the influence of desirable difficulty on retention. Results from Experiment 1 revealed that continued testing in the short lag condition led to consistent increases in retention, whereas continued testing in the long lag condition led to increasingly smaller benefits in retention for both age groups. Results from Experiment 2 revealed that repeated spaced testing enhanced retention relative to taking one spaced test for both age groups; however, repeated massed testing only enhanced retention over taking one test for young adults. Across both experiments, the response latency results were overall consistent with an influence of desirable difficulty on retention. Discussion focuses on the role of desirable difficulty during encoding in producing the benefits of lag, spacing, and testing. PMID:25616776

Dreaming of Atmospheres

NASA Astrophysics Data System (ADS)

Waldmann, Ingo

2016-10-01

Radiative transfer retrievals have become the standard in modelling of exoplanetary transmission and emission spectra. Analysing currently available observations of exoplanetary atmospheres often invoke large and correlated parameter spaces that can be difficult to map or constrain.To address these issues, we have developed the Tau-REx (tau-retrieval of exoplanets) retrieval and the RobERt spectral recognition algorithms. Tau-REx is a bayesian atmospheric retrieval framework using Nested Sampling and cluster computing to fully map these large correlated parameter spaces. Nonetheless, data volumes can become prohibitively large and we must often select a subset of potential molecular/atomic absorbers in an atmosphere.In the era of open-source, automated and self-sufficient retrieval algorithms, such manual input should be avoided. User dependent input could, in worst case scenarios, lead to incomplete models and biases in the retrieval. The RobERt algorithm is build to address these issues. RobERt is a deep belief neural (DBN) networks trained to accurately recognise molecular signatures for a wide range of planets, atmospheric thermal profiles and compositions. Using these deep neural networks, we work towards retrieval algorithms that themselves understand the nature of the observed spectra, are able to learn from current and past data and make sensible qualitative preselections of atmospheric opacities to be used for the quantitative stage of the retrieval process.In this talk I will discuss how neural networks and Bayesian Nested Sampling can be used to solve highly degenerate spectral retrieval problems and what 'dreaming' neural networks can tell us about atmospheric characteristics.

Compact, cost-effective and field-portable microscope prototype based on MISHELF microscopy

NASA Astrophysics Data System (ADS)

Sanz, Martín; Picazo-Bueno, José Ángel; Granero, Luis; García, Javier; Micó, Vicente

2017-02-01

We report on a reduced cost, portable and compact prototype design of lensless holographic microscope with an illumination/detection scheme based on wavelength multiplexing, working with single hologram acquisition and using a fast convergence algorithm for image processing. All together, MISHELF (initials coming from Multi-Illumination Single-Holographic-Exposure Lensless Fresnel) microscopy allows the recording of three Fresnel domain diffraction patterns in a single camera snap-shot incoming from illuminating the sample with three coherent lights at once. Previous implementations have proposed an illumination/detection procedure based on a tuned (illumination wavelengths centered at the maximum sensitivity of the camera detection channels) configuration but here we report on a detuned (non-centered ones) scheme resulting in prototype miniaturization and cost reduction. Thus, MISHELF microscopy in combination with a novel and fast iterative algorithm allows high-resolution (μm range) phase-retrieved (twin image elimination) quantitative phase imaging of dynamic events (video rate recording speed). The performance of this microscope prototype is validated through experiments using both amplitude (USAF resolution test) and complex (live swine sperm cells and flowing microbeads) samples. The proposed method becomes in an alternative instrument improving some capabilities of existing lensless microscopes.

Compact, cost-effective and field-portable microscope prototype based on MISHELF microscopy

PubMed Central

Sanz, Martín; Picazo-Bueno, José Ángel; Granero, Luis; García, Javier; Micó, Vicente

2017-01-01

We report on a reduced cost, portable and compact prototype design of lensless holographic microscope with an illumination/detection scheme based on wavelength multiplexing, working with single hologram acquisition and using a fast convergence algorithm for image processing. All together, MISHELF (initials coming from Multi-Illumination Single-Holographic-Exposure Lensless Fresnel) microscopy allows the recording of three Fresnel domain diffraction patterns in a single camera snap-shot incoming from illuminating the sample with three coherent lights at once. Previous implementations have proposed an illumination/detection procedure based on a tuned (illumination wavelengths centered at the maximum sensitivity of the camera detection channels) configuration but here we report on a detuned (non-centered ones) scheme resulting in prototype miniaturization and cost reduction. Thus, MISHELF microscopy in combination with a novel and fast iterative algorithm allows high-resolution (μm range) phase-retrieved (twin image elimination) quantitative phase imaging of dynamic events (video rate recording speed). The performance of this microscope prototype is validated through experiments using both amplitude (USAF resolution test) and complex (live swine sperm cells and flowing microbeads) samples. The proposed method becomes in an alternative instrument improving some capabilities of existing lensless microscopes. PMID:28233829

X-ray fast tomography and its applications in dynamical phenomena studies in geosciences at Advanced Photon Source

NASA Astrophysics Data System (ADS)

Xiao, Xianghui; Fusseis, Florian; De Carlo, Francesco

2012-10-01

State-of-art synchrotron radiation based micro-computed tomography provides high spatial and temporal resolution. This matches the needs of many research problems in geosciences. In this letter we report the current capabilities in microtomography at sector 2BM at the Advanced Photon Source (APS) of Argonne National Laboratory. The beamline is well suited to routinely acquire three-dimensional data of excellent quality with sub-micron resolution. Fast cameras in combination with a polychromatic beam allow time-lapse experiments with temporal resolutions of down to 200 ms. Data processing utilizes quantitative phase retrieval to optimize contrast in phase contrast tomographic data. The combination of these capabilities with purpose-designed experimental cells allows for a wide range of dynamic studies on geoscientific topics, two of which are summarized here. In the near future, new experimental cells capable of simulating conditions in most geological reservoirs will be available for general use. Ultimately, these advances will be matched by a new wide-field imaging beam line, which will be constructed as part of the APS upgrade. It is expected that even faster tomography with larger field of view can be conducted at this beam line, creating new opportunities for geoscientific studies.

Size distribution and scattering phase function of aerosol particles retrieved from sky brightness measurements

NASA Technical Reports Server (NTRS)

Kaufman, Y. J.; Gitelson, A.; Karnieli, A.; Ganor, E. (Editor); Fraser, R. S.; Nakajima, T.; Mattoo, S.; Holben, B. N.

1994-01-01

Ground-based measurements of the solar transmission and sky radiance in a horizontal plane through the Sun are taken in several geographical regions and aerosol types: dust in a desert transition zone in Israel, sulfate particles in Eastern and Western Europe, tropical aerosol in Brazil, and mixed continental/maritime aerosol in California. Stratospheric aerosol was introduced after the eruption of Mount Pinatubo in June 1991. Therefore measurements taken before the eruption are used to analyze the properties of tropospheric aerosol; measurements from 1992 are also used to detect the particle size and concentration of stratospheric aerosol. The measurements are used to retrieve the size distribution and the scattering phase function at large scattering angles of the undisturbed aerosol particles. The retrieved properties represent an average on the entire atmospheric column. A comparison between the retrieved phase function for a scattering angle of 120 deg, with phase function predicted from the retrieved size distribution, is used to test the assumption of particle homogeneity and sphericity in radiative transfer models (Mie theory). The effect was found to be small (20% +/- 15%). For the stratospheric aerosol (sulfates), as expected, the phase function was very well predicted using the Mie theory. A model with a power law distribution, based on the spectral dependence of the optical thickness, alpha, cannot estimate accurately the phase function (up to 50% error for lambda = 0.87 microns). Before the Pinatubo eruption the ratio between the volumes of sulfate and coarse particles was very well correlated with alpha. The Pinatubo stratospheric aerosol destroyed this correlation. The aerosol optical properties are compared with analysis of the size, shape, and composition of the individual particles by electron microscopy of in situ samples. The measured volume size distribution before the injection of stratospheric aerosol consistently show two modes, sulfate particles with r(sub m) less than 0.2 microns and coarse paritcles with r(sub m) greater than 0.7 microns. The 'window' in the tropospheric aerosol in this radius range was used to observe a stable stratospheric aerosol in 1992, with r(sub m) approximately 0.5 microns. A combination of such optical thickness and sky measurements can be used to assess the direct forcing and the climatic impact of aerosol. Systematic inversion for the key aerosol types (sulfates, smoke, dust, and maritime aerosol) of the size distribution and phase function can give the relationship between the aerosol physical and optical properties that can be used to compute the radiative forcing. This forcing can be validated in dedicated field experiments.

Decomposing the interaction between retention interval and study/test practice: the role of retrievability.

PubMed

Jang, Yoonhee; Wixted, John T; Pecher, Diane; Zeelenberg, René; Huber, David E

2012-01-01

Even without feedback, test practice enhances delayed performance compared to study practice, but the size of the effect is variable across studies. We investigated the benefit of testing, separating initially retrievable items from initially nonretrievable items. In two experiments, an initial test determined item retrievability. Retrievable or nonretrievable items were subsequently presented for repeated study or test practice. Collapsing across items, in Experiment 1, we obtained the typical cross-over interaction between retention interval and practice type. For retrievable items, however, the cross-over interaction was quantitatively different, with a small study benefit for an immediate test and a larger testing benefit after a delay. For nonretrievable items, there was a large study benefit for an immediate test, but one week later there was no difference between the study and test practice conditions. In Experiment 2, initially nonretrievable items were given additional study followed by either an immediate test or even more additional study, and one week later performance did not differ between the two conditions. These results indicate that the effect size of study/test practice is due to the relative contribution of retrievable and nonretrievable items.

Stratospheric and mesospheric pressure-temperature profiles from rotational analysis of CO2 lines in atmospheric trace molecule spectroscopy/ATLAS 1 infrared solar occultation spectra

NASA Technical Reports Server (NTRS)

Stiller, G. P.; Gunson, M. R.; Lowes, L. L.; Abrams, M. C.; Raper, O. F.; Farmer, C. B.; Zander, R.; Rinsland, C. P.

1995-01-01

A simple, classical, and expedient method for the retrieval of atmospheric pressure-temperature profiles has been applied to the high-resolution infrared solar absorption spectra obtained with the atmospheric trace molecule spectroscopy (ATMOS) instrument. The basis for this method is a rotational analysis of retrieved apparent abundances from CO2 rovibrational absorption lines, employing existing constituent concentration retrieval software used in the analysis of data returned by ATMOS. Pressure-temperature profiles derived from spectra acquired during the ATLAS 1 space shuttle mission of March-April 1992 are quantitatively evaluated and compared with climatological and meteorological data as a means of assessing the validity of this approach.

Characterizations of individual human red blood cells from patients with diabetes mellitus (Conference Presentation)

NASA Astrophysics Data System (ADS)

Lee, SangYun; Jang, Seongsoo; Park, HyunJoo; Park, YongKeun

2016-03-01

We systematically measure the morphological, biochemical, and biomechanical properties of individual human red blood cells (RBCs) from patients with diabetes mellitus using quantitative phase imaging technique to characterize the diabetic red cells with respect to those of the healthy. The 3-D refractive index tomograms and 2-D dynamic membrane fluctuation maps of individual RBCs are reconstructed from a set of the retrieved complex optical fields at various laser incidence angles using the Common-path diffraction optical tomography, from which volume, surface area, sphericity, hemoglobin (Hb) concentration, Hb content, and membrane fluctuation are obtained simultaneously. The correlative relations among the retrieved red cell indices of diabetic and healthy RBCs are also investigated with capabilities of individual cell measurement. As expected, there are no significant alterations in morphologies (cellular volumes, surface area, and sphericity) between diabetic and healthy RBCs. However, despite the minute mean corpuscular Hb differences in cell blood count datasheet, the measured Hb concentrations and Hb contents of diabetic RBCs are statistically higher than those of healthy RBCs, which might be related to the glycation of Hb molecules by hyperglycemia. Meanwhile, the membrane fluctuations of diabetic RBCs are clearly diminished compared to healthy red cells, implying the significantly decreased RBC deformability. In particular, it seems that the membrane fluctuations have mild negative relationships with the reported HbA1c levels.

Integrating uncertainty propagation in GNSS radio occultation retrieval: from excess phase to atmospheric bending angle profiles

NASA Astrophysics Data System (ADS)

Schwarz, Jakob; Kirchengast, Gottfried; Schwaerz, Marc

2018-05-01

Global Navigation Satellite System (GNSS) radio occultation (RO) observations are highly accurate, long-term stable data sets and are globally available as a continuous record from 2001. Essential climate variables for the thermodynamic state of the free atmosphere - such as pressure, temperature, and tropospheric water vapor profiles (involving background information) - can be derived from these records, which therefore have the potential to serve as climate benchmark data. However, to exploit this potential, atmospheric profile retrievals need to be very accurate and the remaining uncertainties quantified and traced throughout the retrieval chain from raw observations to essential climate variables. The new Reference Occultation Processing System (rOPS) at the Wegener Center aims to deliver such an accurate RO retrieval chain with integrated uncertainty propagation. Here we introduce and demonstrate the algorithms implemented in the rOPS for uncertainty propagation from excess phase to atmospheric bending angle profiles, for estimated systematic and random uncertainties, including vertical error correlations and resolution estimates. We estimated systematic uncertainty profiles with the same operators as used for the basic state profiles retrieval. The random uncertainty is traced through covariance propagation and validated using Monte Carlo ensemble methods. The algorithm performance is demonstrated using test day ensembles of simulated data as well as real RO event data from the satellite missions CHAllenging Minisatellite Payload (CHAMP); Constellation Observing System for Meteorology, Ionosphere, and Climate (COSMIC); and Meteorological Operational Satellite A (MetOp). The results of the Monte Carlo validation show that our covariance propagation delivers correct uncertainty quantification from excess phase to bending angle profiles. The results from the real RO event ensembles demonstrate that the new uncertainty estimation chain performs robustly. Together with the other parts of the rOPS processing chain this part is thus ready to provide integrated uncertainty propagation through the whole RO retrieval chain for the benefit of climate monitoring and other applications.

Competitive Semantic Memory Retrieval: Temporal Dynamics Revealed by Event-Related Potentials

PubMed Central

Hellerstedt, Robin; Johansson, Mikael

2016-01-01

Memories compete for retrieval when they are related to a common retrieval cue. Previous research has shown that retrieval of a target memory may lead to subsequent retrieval-induced forgetting (RIF) of currently irrelevant competing memories. In the present study, we investigated the time course of competitive semantic retrieval and examined the neurocognitive mechanisms underlying RIF. We contrasted two theoretical accounts of RIF by examining a critical aspect of this memory phenomenon, namely the extent to which it depends on successful retrieval of the target memory. Participants first studied category-exemplar word-pairs (e.g. Fruit—Apple). Next, we recorded electrophysiological measures of brain activity while the participants performed a competitive semantic cued-recall task. In this task, the participants were provided with the studied categories but they were instructed to retrieve other unstudied exemplars (e.g. Fruit—Ma__?). We investigated the event-related potential (ERP) correlates of retrieval success by comparing ERPs from successful and failed retrieval trials. To isolate the ERP correlates of continuous retrieval attempts from the ERP correlates of retrieval success, we included an impossible retrieval condition, with incompletable word-stem cues (Drinks—Wy__) and compared it with a non-retrieval presentation baseline condition (Occupation—Dentist). The participants’ memory for all the studied exemplars was tested in the final phase of the experiment. Taken together, the behavioural results suggest that RIF is independent of target retrieval. Beyond investigating the mechanisms underlying RIF, the present study also elucidates the temporal dynamics of semantic cued-recall by isolating the ERP correlates of retrieval attempt and retrieval success. The ERP results revealed that retrieval attempt is reflected in a late posterior negativity, possibly indicating construction of candidates for completing the word-stem cue and retrieval monitoring whereas retrieval success was reflected in an anterior positive slow wave. PMID:26901865

Competitive Semantic Memory Retrieval: Temporal Dynamics Revealed by Event-Related Potentials.

PubMed

Hellerstedt, Robin; Johansson, Mikael

2016-01-01

Memories compete for retrieval when they are related to a common retrieval cue. Previous research has shown that retrieval of a target memory may lead to subsequent retrieval-induced forgetting (RIF) of currently irrelevant competing memories. In the present study, we investigated the time course of competitive semantic retrieval and examined the neurocognitive mechanisms underlying RIF. We contrasted two theoretical accounts of RIF by examining a critical aspect of this memory phenomenon, namely the extent to which it depends on successful retrieval of the target memory. Participants first studied category-exemplar word-pairs (e.g. Fruit-Apple). Next, we recorded electrophysiological measures of brain activity while the participants performed a competitive semantic cued-recall task. In this task, the participants were provided with the studied categories but they were instructed to retrieve other unstudied exemplars (e.g. Fruit-Ma__?). We investigated the event-related potential (ERP) correlates of retrieval success by comparing ERPs from successful and failed retrieval trials. To isolate the ERP correlates of continuous retrieval attempts from the ERP correlates of retrieval success, we included an impossible retrieval condition, with incompletable word-stem cues (Drinks-Wy__) and compared it with a non-retrieval presentation baseline condition (Occupation-Dentist). The participants' memory for all the studied exemplars was tested in the final phase of the experiment. Taken together, the behavioural results suggest that RIF is independent of target retrieval. Beyond investigating the mechanisms underlying RIF, the present study also elucidates the temporal dynamics of semantic cued-recall by isolating the ERP correlates of retrieval attempt and retrieval success. The ERP results revealed that retrieval attempt is reflected in a late posterior negativity, possibly indicating construction of candidates for completing the word-stem cue and retrieval monitoring whereas retrieval success was reflected in an anterior positive slow wave.

Propagation phasor approach for holographic image reconstruction

PubMed Central

Luo, Wei; Zhang, Yibo; Göröcs, Zoltán; Feizi, Alborz; Ozcan, Aydogan

2016-01-01

To achieve high-resolution and wide field-of-view, digital holographic imaging techniques need to tackle two major challenges: phase recovery and spatial undersampling. Previously, these challenges were separately addressed using phase retrieval and pixel super-resolution algorithms, which utilize the diversity of different imaging parameters. Although existing holographic imaging methods can achieve large space-bandwidth-products by performing pixel super-resolution and phase retrieval sequentially, they require large amounts of data, which might be a limitation in high-speed or cost-effective imaging applications. Here we report a propagation phasor approach, which for the first time combines phase retrieval and pixel super-resolution into a unified mathematical framework and enables the synthesis of new holographic image reconstruction methods with significantly improved data efficiency. In this approach, twin image and spatial aliasing signals, along with other digital artifacts, are interpreted as noise terms that are modulated by phasors that analytically depend on the lateral displacement between hologram and sensor planes, sample-to-sensor distance, wavelength, and the illumination angle. Compared to previous holographic reconstruction techniques, this new framework results in five- to seven-fold reduced number of raw measurements, while still achieving a competitive resolution and space-bandwidth-product. We also demonstrated the success of this approach by imaging biological specimens including Papanicolaou and blood smears. PMID:26964671

Information hiding and retrieval in Rydberg wave packets using half-cycle pulses

DOE Office of Scientific and Technical Information (OSTI.GOV)

Murray, J. M.; Pisharody, S. N.; Wen, H.

We demonstrate an information hiding and retrieval scheme with the relative phases between states in a Rydberg wave packet acting as the bits of a data register. We use a terahertz half-cycle pulse (HCP) to transfer phase-encoded information from an optically accessible angular momentum manifold to another manifold which is not directly accessed by our laser pulses, effectively hiding the information from our optical interferometric measurement techniques. A subsequent HCP acting on these wave packets reintroduces the information back into the optically accessible data register manifold which can then be read out.

Performance assessment of femoral knee components made from cobalt-chromium alloy and oxidized zirconium.

PubMed

Brandt, J-M; Guenther, L; O'Brien, S; Vecherya, A; Turgeon, T R; Bohm, E R

2013-12-01

The surface characteristics of the femoral component affect polyethylene wear in modular total knee replacements. In the present retrieval study, the surface characteristics of cobalt-chromium (CoCr) alloy and oxidized zirconium (OxZr) femoral components were assessed and compared. Twenty-six retrieved CoCr alloy femoral components were matched with twenty-six retrieved OxZr femoral components for implantation period, body-mass index, patient gender, implant type, and polyethylene insert thickness. The surface damage on the retrieved femoral components was evaluated using a semi-quantitative assessment method, scanning electron microscopy, and contact profilometry. The retrieved CoCr alloy femoral components showed less posterior surface gouging than OxZr femoral components; however, at a higher magnification, the grooving damage features on the retrieved CoCr alloy femoral components confirmed an abrasive wear mechanism. The surface roughness values Rp, Rpm, and Rpk for the retrieved CoCr alloy femoral components were found to be significantly higher than those of the retrieved OxZr femoral components (p≤0.031). The surface roughness values were higher on the medial condyles than on the lateral condyles of the retrieved CoCr alloy femoral components; such a difference was not observed on the retrieved OxZr femoral components. The surface roughness of CoCr alloy femoral components increased while the surface roughness of the OxZr femoral components remained unchanged after in vivo service. Therefore, the OxZr femoral components' resistance to abrasive wear may enable lower polyethylene wear and ensure long-term durability in vivo. Level IV. Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.

3D displacement time series in the Afar rift zone computed from SAR phase and amplitude information

NASA Astrophysics Data System (ADS)

Casu, Francesco; Manconi, Andrea

2013-04-01

Large and rapid deformations, such as those caused by earthquakes, eruptions, and landslides cannot be fully measured by using standard DInSAR applications. Indeed, the phase information often degrades and some areas of the interferograms are affected by high fringe rates, leading to difficulties in the phase unwrapping, and/or to complete loss of coherence due to significant misregistration errors. This limitation can be overcome by exploiting the SAR image amplitude information instead of the phase, and by calculating the Pixel-Offset (PO) field SAR image pairs, for both range and azimuth directions. Moreover, it is possible to combine the PO results by following the same rationale of the SBAS technique, to finally retrieve the offset-based deformation time series. Such technique, named PO-SBAS, permits to retrieve the deformation field in areas affected by very large displacements at an accuracy that, for ENVISAT data, correspond to 30 cm and 15 cm for the range and azimuth, respectively [1]. Moreover, the combination of SBAS and PO-SBAS time series can help to better study and model deformation phenomena characterized by spatial and temporal heterogeneities [2]. The Dabbahu rift segment of the Afar depression has been active since 2005 when a 2.5 km3 dyke intrusion and hundreds of earthquakes marked the onset a rifting episode which continues to date. The ENVISAT satellite has repeatedly imaged the Afar depression since 2003, generating a large SAR archive. In this work, we study the Afar rift region deformations by using both the phase and amplitude information of several sets of SAR images acquired from ascending and descending ENVISAT tracks. We combined sets of small baseline interferograms through the SBAS algorithm, and we generate both ground deformation maps and time series along the satellite Line-Of-Sight (LOS). In areas where the deformation gradient causes loss of coherence, we retrieve the displacement field through the amplitude information. Furthermore, we could also retrieve the full 3D deformation field, by considering the North-South displacement component obtained from the azimuth PO information. The combination of SBAS and PO-SBAS information permits to better retrieve and constrain the full deformation field due to repeated intrusions, fault movements, as well as the magma movements from individual magma chambers. [1] Casu, F., A. Manconi, A. Pepe and R. Lanari, 2011. Deformation time-series generation in areas characterized by large displacement dynamics: the SAR amplitude Pixel-Offset SBAS technique, IEEE Transaction on Geosciences and Remote Sensing. [2] Manconi, A. and F. Casu, 2012. Joint analysis of displacement time series retrieved from SAR phase and amplitude: impact on the estimation of volcanic source parameters, Geophysical Research Letters, doi:10.1029/2012GL052202.

Improvement of encoding and retrieval in normal and pathological aging with word-picture paradigm.

PubMed

Iodice, Rosario; Meilán, Juan José G; Carro, Juan

2015-01-01

During the aging process, there is a progressive deficit in the encoding of new information and its retrieval. Different strategies are used in order to maintain, optimize or diminish these deficits in people with and without dementia. One of the classic techniques is paired-associate learning (PAL), which is based on improving the encoding of memories, but it has yet to be used to its full potential in people with dementia. In this study, our aim is to corroborate the importance of PAL tasks as instrumental tools for creating contextual cues, during both the encoding and retrieval phases of memory. Additionally, we aim to identify the most effective form of presenting the related items. Pairs of stimuli were shown to healthy elderly people and to patients with moderate and mild Alzheimer's disease. The encoding conditions were as follows: word/word, picture/picture, picture/word, and word/picture. Associative cued recall of the second item in the pair shows that retrieval is higher for the word/picture condition in the two groups of patients with dementia when compared to the other conditions, while word/word is the least effective in all cases. These results confirm that PAL is an effective tool for creating contextual cues during both the encoding and retrieval phases in people with dementia when the items are presented using the word/picture condition. In this way, the encoding and retrieval deficit can be reduced in these people.

Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders

PubMed Central

Marquet, Pierre; Depeursinge, Christian; Magistretti, Pierre J.

2014-01-01

Abstract. Quantitative phase microscopy (QPM) has recently emerged as a new powerful quantitative imaging technique well suited to noninvasively explore a transparent specimen with a nanometric axial sensitivity. In this review, we expose the recent developments of quantitative phase-digital holographic microscopy (QP-DHM). Quantitative phase-digital holographic microscopy (QP-DHM) represents an important and efficient quantitative phase method to explore cell structure and dynamics. In a second part, the most relevant QPM applications in the field of cell biology are summarized. A particular emphasis is placed on the original biological information, which can be derived from the quantitative phase signal. In a third part, recent applications obtained, with QP-DHM in the field of cellular neuroscience, namely the possibility to optically resolve neuronal network activity and spine dynamics, are presented. Furthermore, potential applications of QPM related to psychiatry through the identification of new and original cell biomarkers that, when combined with a range of other biomarkers, could significantly contribute to the determination of high risk developmental trajectories for psychiatric disorders, are discussed. PMID:26157976

Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders.

PubMed

Marquet, Pierre; Depeursinge, Christian; Magistretti, Pierre J

2014-10-01

Quantitative phase microscopy (QPM) has recently emerged as a new powerful quantitative imaging technique well suited to noninvasively explore a transparent specimen with a nanometric axial sensitivity. In this review, we expose the recent developments of quantitative phase-digital holographic microscopy (QP-DHM). Quantitative phase-digital holographic microscopy (QP-DHM) represents an important and efficient quantitative phase method to explore cell structure and dynamics. In a second part, the most relevant QPM applications in the field of cell biology are summarized. A particular emphasis is placed on the original biological information, which can be derived from the quantitative phase signal. In a third part, recent applications obtained, with QP-DHM in the field of cellular neuroscience, namely the possibility to optically resolve neuronal network activity and spine dynamics, are presented. Furthermore, potential applications of QPM related to psychiatry through the identification of new and original cell biomarkers that, when combined with a range of other biomarkers, could significantly contribute to the determination of high risk developmental trajectories for psychiatric disorders, are discussed.

Enamel color changes following orthodontic treatment.

PubMed

Pandian, Akshaya; Ranganathan, Sukanya; Padmanabhan, Sridevi

2017-01-01

To evaluate and compare the effect of various orthodontic bonding systems and clean up procedures on quantitative enamel colour change. A literature search was done to identify the studies that assessed the quantitative enamel colour change associated with the various bonding systems and cleanup procedures. Electronic database (Pub Med, Cochrane and Google Scholar) were searched. First stage screening was performed and the abstracts were selected according to the initial selection criteria. Full text articles were retrieved and analyzed during second stage screening. The bibliographies were reviewed to identify additional relevant studies. Sixteen full text articles were retrieved. Six were rejected because the methodology was different. There was significant enamel colour change following orthodontic bonding, debonding and clean up procedures. Self-etching primers produce less enamel colour change compared to conventional etching. Resin Modified GIC produces least colour change compared to other light cure and chemical cure systems. Polishing following the clean-up procedure reduces the colour change of the enamel.

On iterative algorithms for quantitative photoacoustic tomography in the radiative transport regime

NASA Astrophysics Data System (ADS)

Wang, Chao; Zhou, Tie

2017-11-01

In this paper, we present a numerical reconstruction method for quantitative photoacoustic tomography (QPAT), based on the radiative transfer equation (RTE), which models light propagation more accurately than diffusion approximation (DA). We investigate the reconstruction of absorption coefficient and scattering coefficient of biological tissues. An improved fixed-point iterative method to retrieve the absorption coefficient, given the scattering coefficient, is proposed for its cheap computational cost; the convergence of this method is also proved. The Barzilai-Borwein (BB) method is applied to retrieve two coefficients simultaneously. Since the reconstruction of optical coefficients involves the solutions of original and adjoint RTEs in the framework of optimization, an efficient solver with high accuracy is developed from Gao and Zhao (2009 Transp. Theory Stat. Phys. 38 149-92). Simulation experiments illustrate that the improved fixed-point iterative method and the BB method are competitive methods for QPAT in the relevant cases.

Demonstration of a diode-laser-based high spectral resolution lidar (HSRL) for quantitative profiling of clouds and aerosols.

PubMed

Hayman, Matthew; Spuler, Scott

2017-11-27

We present a demonstration of a diode-laser-based high spectral resolution lidar. It is capable of performing calibrated retrievals of aerosol and cloud optical properties at a 150 m range resolution with less than 1 minute integration time over an approximate range of 12 km during day and night. This instrument operates at 780 nm, a wavelength that is well established for reliable semiconductor lasers and detectors, and was chosen because it corresponds to the D2 rubidium absorption line. A heated vapor reference cell of isotopic rubidium 87 is used as an effective and reliable aerosol signal blocking filter in the instrument. In principle, the diode-laser-based high spectral resolution lidar can be made cost competitive with elastic backscatter lidar systems, yet delivers a significant improvement in data quality through direct retrieval of quantitative optical properties of clouds and aerosols.

Closed loop adaptive optics for microscopy without a wavefront sensor.

PubMed

Kner, Peter; Winoto, Lukman; Agard, David A; Sedat, John W

2010-02-24

A three-dimensional wide-field image of a small fluorescent bead contains more than enough information to accurately calculate the wavefront in the microscope objective back pupil plane using the phase retrieval technique. The phase-retrieved wavefront can then be used to set a deformable mirror to correct the point-spread function (PSF) of the microscope without the use of a wavefront sensor. This technique will be useful for aligning the deformable mirror in a widefield microscope with adaptive optics and could potentially be used to correct aberrations in samples where small fluorescent beads or other point sources are used as reference beacons. Another advantage is the high resolution of the retrieved wavefont as compared with current Shack-Hartmann wavefront sensors. Here we demonstrate effective correction of the PSF in 3 iterations. Starting from a severely aberrated system, we achieve a Strehl ratio of 0.78 and a greater than 10-fold increase in maximum intensity.

Enhanced dual-frequency pattern scheme based on spatial-temporal fringes method

NASA Astrophysics Data System (ADS)

Wang, Minmin; Zhou, Canlin; Si, Shuchun; Lei, Zhenkun; Li, Xiaolei; Li, Hui; Li, YanJie

2018-07-01

One of the major challenges of employing a dual-frequency phase-shifting algorithm for phase retrieval is its sensitivity to noise. Yun et al proposed a dual-frequency method based on the Fourier transform profilometry, yet the low-frequency lobes are close to each other for accurate band-pass filtering. In the light of this problem, a novel dual-frequency pattern based on the spatial-temporal fringes (STF) method is developed in this paper. Three fringe patterns with two different frequencies are required. The low-frequency phase is obtained from two low-frequency fringe patterns by the STF method, so the signal lobes can be extracted accurately as they are far away from each other. The high-frequency phase is retrieved from another fringe pattern without the impact of the DC component. Simulations and experiments are conducted to demonstrate the excellent precision of the proposed method.

Reliable absolute analog code retrieval approach for 3D measurement

NASA Astrophysics Data System (ADS)

Yu, Shuang; Zhang, Jing; Yu, Xiaoyang; Sun, Xiaoming; Wu, Haibin; Chen, Deyun

2017-11-01

The wrapped phase of phase-shifting approach can be unwrapped by using Gray code, but both the wrapped phase error and Gray code decoding error can result in period jump error, which will lead to gross measurement error. Therefore, this paper presents a reliable absolute analog code retrieval approach. The combination of unequal-period Gray code and phase shifting patterns at high frequencies are used to obtain high-frequency absolute analog code, and at low frequencies, the same unequal-period combination patterns are used to obtain the low-frequency absolute analog code. Next, the difference between the two absolute analog codes was employed to eliminate period jump errors, and a reliable unwrapped result can be obtained. Error analysis was used to determine the applicable conditions, and this approach was verified through theoretical analysis. The proposed approach was further verified experimentally. Theoretical analysis and experimental results demonstrate that the proposed approach can perform reliable analog code unwrapping.

DREAMING OF ATMOSPHERES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Waldmann, I. P., E-mail: ingo@star.ucl.ac.uk

Here, we introduce the RobERt (Robotic Exoplanet Recognition) algorithm for the classification of exoplanetary emission spectra. Spectral retrieval of exoplanetary atmospheres frequently requires the preselection of molecular/atomic opacities to be defined by the user. In the era of open-source, automated, and self-sufficient retrieval algorithms, manual input should be avoided. User dependent input could, in worst-case scenarios, lead to incomplete models and biases in the retrieval. The RobERt algorithm is based on deep-belief neural (DBN) networks trained to accurately recognize molecular signatures for a wide range of planets, atmospheric thermal profiles, and compositions. Reconstructions of the learned features, also referred to as themore » “dreams” of the network, indicate good convergence and an accurate representation of molecular features in the DBN. Using these deep neural networks, we work toward retrieval algorithms that themselves understand the nature of the observed spectra, are able to learn from current and past data, and make sensible qualitative preselections of atmospheric opacities to be used for the quantitative stage of the retrieval process.« less

Direct single-shot phase retrieval from the diffraction pattern of separated objects

DOE Office of Scientific and Technical Information (OSTI.GOV)

Leshem, Ben; Xu, Rui; Dallal, Yehonatan

The non-crystallographic phase problem arises in numerous scientific and technological fields. An important application is coherent diffractive imaging. Recent advances in X-ray free-electron lasers allow capturing of the diffraction pattern from a single nanoparticle before it disintegrates, in so-called ‘diffraction before destruction’ experiments. Presently, the phase is reconstructed by iterative algorithms, imposing a non-convex computational challenge, or by Fourier holography, requiring a well-characterized reference field. Here we present a convex scheme for single-shot phase retrieval for two (or more) sufficiently separated objects, demonstrated in two dimensions. In our approach, the objects serve as unknown references to one another, reducing themore » phase problem to a solvable set of linear equations. We establish our method numerically and experimentally in the optical domain and demonstrate a proof-of-principle single-shot coherent diffractive imaging using X-ray free-electron lasers pulses. Lastly, our scheme alleviates several limitations of current methods, offering a new pathway towards direct reconstruction of complex objects.« less

Direct single-shot phase retrieval from the diffraction pattern of separated objects

DOE PAGES

Leshem, Ben; Xu, Rui; Dallal, Yehonatan; ...

2016-02-22

The non-crystallographic phase problem arises in numerous scientific and technological fields. An important application is coherent diffractive imaging. Recent advances in X-ray free-electron lasers allow capturing of the diffraction pattern from a single nanoparticle before it disintegrates, in so-called ‘diffraction before destruction’ experiments. Presently, the phase is reconstructed by iterative algorithms, imposing a non-convex computational challenge, or by Fourier holography, requiring a well-characterized reference field. Here we present a convex scheme for single-shot phase retrieval for two (or more) sufficiently separated objects, demonstrated in two dimensions. In our approach, the objects serve as unknown references to one another, reducing themore » phase problem to a solvable set of linear equations. We establish our method numerically and experimentally in the optical domain and demonstrate a proof-of-principle single-shot coherent diffractive imaging using X-ray free-electron lasers pulses. Lastly, our scheme alleviates several limitations of current methods, offering a new pathway towards direct reconstruction of complex objects.« less

Fringe pattern information retrieval using wavelets

NASA Astrophysics Data System (ADS)

Sciammarella, Cesar A.; Patimo, Caterina; Manicone, Pasquale D.; Lamberti, Luciano

2005-08-01

Two-dimensional phase modulation is currently the basic model used in the interpretation of fringe patterns that contain displacement information, moire, holographic interferometry, speckle techniques. Another way to look to these two-dimensional signals is to consider them as frequency modulated signals. This alternative interpretation has practical implications similar to those that exist in radio engineering for handling frequency modulated signals. Utilizing this model it is possible to obtain frequency information by using the energy approach introduced by Ville in 1944. A natural complementary tool of this process is the wavelet methodology. The use of wavelet makes it possible to obtain the local values of the frequency in a one or two dimensional domain without the need of previous phase retrieval and differentiation. Furthermore from the properties of wavelets it is also possible to obtain at the same time the phase of the signal with the advantage of a better noise removal capabilities and the possibility of developing simpler algorithms for phase unwrapping due to the availability of the derivative of the phase.

Known plaintext attack on double random phase encoding using fingerprint as key and a method for avoiding the attack.

PubMed

Tashima, Hideaki; Takeda, Masafumi; Suzuki, Hiroyuki; Obi, Takashi; Yamaguchi, Masahiro; Ohyama, Nagaaki

2010-06-21

We have shown that the application of double random phase encoding (DRPE) to biometrics enables the use of biometrics as cipher keys for binary data encryption. However, DRPE is reported to be vulnerable to known-plaintext attacks (KPAs) using a phase recovery algorithm. In this study, we investigated the vulnerability of DRPE using fingerprints as cipher keys to the KPAs. By means of computational experiments, we estimated the encryption key and restored the fingerprint image using the estimated key. Further, we propose a method for avoiding the KPA on the DRPE that employs the phase retrieval algorithm. The proposed method makes the amplitude component of the encrypted image constant in order to prevent the amplitude component of the encrypted image from being used as a clue for phase retrieval. Computational experiments showed that the proposed method not only avoids revealing the cipher key and the fingerprint but also serves as a sufficiently accurate verification system.

The Effect of Retrieval Cues on Visual Preferences and Memory in Infancy: Evidence for a Four-Phase Attention Function.

ERIC Educational Resources Information Center

Bahrick, Lorraine E.; Hernandez-Reif, Maria; Pickens, Jeffrey N.

1997-01-01

Tested hypothesis from Bahrick and Pickens' infant attention model that retrieval cues increase memory accessibility and shift visual preferences toward greater novelty to resemble recent memories. Found that after retention intervals associated with remote or intermediate memory, previous familiarity preferences shifted to null or novelty…

Contextual Cues Aid Recovery from Interruption: The Role of Associative Activation

ERIC Educational Resources Information Center

Hodgetts, Helen M.; Jones, Dylan M.

2006-01-01

A series of experiments introduced interruptions to the execution phase of simple Tower of London problems and found that the opportunity for preparation before the break in task reduced the time cost at resumption. Retrieval of the suspended goal was facilitated when participants were given the opportunity to encode retrieval cues during an…

Using Paraffin PCM, Cryogel and TEC to Maintain Comet Surface Sample Cold from Earth Approach Through Retrieval

NASA Technical Reports Server (NTRS)

Choi, Michael K.

2017-01-01

An innovative thermal design concept to maintain comet surface samples cold (for example, 263 degrees Kelvin, 243 degrees Kelvin or 223 degrees Kelvin) from Earth approach through retrieval is presented. It uses paraffin phase change material (PCM), Cryogel insulation and thermoelectric cooler (TEC), which are commercially available.

Retrieval-induced forgetting without competition: testing the retrieval specificity assumption of the inhibition theory.

PubMed

Raaijmakers, Jeroen G W; Jakab, Emoke

2012-01-01

According to the inhibition theory of forgetting (Anderson, Journal of Memory and Language 49:415-445, 2003; Anderson, Bjork, & Bjork, Psychonomic Bulletin & Review 7:522-530, 2000), retrieval practice on a subset of target items leads to forgetting for the other, nontarget items, due to the fact that these other items interfere during the retrieval process and have to be inhibited in order to resolve the interference. In this account, retrieval-induced forgetting occurs only when competition takes place between target and nontarget items during target item practice, since only in such a case is inhibition of the nontarget items necessary. Strengthening of the target item without active retrieval should not lead to such an impairment. In two experiments, we investigated this assumption by using noncompetitive retrieval during the practice phase. We strengthened the cue-target item association during practice by recall of the category name instead of the target item, and thus eliminated competition between the different item types (as in Anderson et al., Psychonomic Bulletin & Review 7:522-530 2000). In contrast to the expectations of the inhibition theory, retrieval-induced forgetting occurred even without competition, and thus the present study does not support the retrieval specificity assumption.

The Asymmetrical Effects of Divided Attention on Encoding and Retrieval Processes: A Different View Based on an Interference with the Episodic Register

PubMed Central

Guez, Jonathan; Naveh-Benjamin, Moshe

2013-01-01

In this study, we evaluate the conceptualization of encoding and retrieval processes established in previous studies that used a divided attention (DA) paradigm. These studies indicated that there were considerable detrimental effects of DA at encoding on later memory performance, but only minimal effects, if any, on divided attention at retrieval. We suggest that this asymmetry in the effects of DA on memory can be due, at least partially, to a confound between the memory phase (encoding and retrieval) and the memory requirements of the task (memory “for” encoded information versus memory “at” test). To control for this confound, we tested memory for encoded information and for retrieved information by introducing a second test that assessed memory for the retrieved information from the first test. We report the results of four experiments that use measures of memory performance, retrieval latency, and performance on the concurrent task, all of which consistently show that DA at retrieval strongly disrupts later memory for the retrieved episode, similarly to the effects of DA at encoding. We suggest that these symmetrical disruptive effects of DA at encoding and retrieval on later retrieval reflect a disruption of an episodic buffer (EB) or episodic register component (ER), rather than a failure of encoding or retrieval operations per se. PMID:24040249

The asymmetrical effects of divided attention on encoding and retrieval processes: a different view based on an interference with the episodic register.

PubMed

Guez, Jonathan; Naveh-Benjamin, Moshe

2013-01-01

In this study, we evaluate the conceptualization of encoding and retrieval processes established in previous studies that used a divided attention (DA) paradigm. These studies indicated that there were considerable detrimental effects of DA at encoding on later memory performance, but only minimal effects, if any, on divided attention at retrieval. We suggest that this asymmetry in the effects of DA on memory can be due, at least partially, to a confound between the memory phase (encoding and retrieval) and the memory requirements of the task (memory "for" encoded information versus memory "at" test). To control for this confound, we tested memory for encoded information and for retrieved information by introducing a second test that assessed memory for the retrieved information from the first test. We report the results of four experiments that use measures of memory performance, retrieval latency, and performance on the concurrent task, all of which consistently show that DA at retrieval strongly disrupts later memory for the retrieved episode, similarly to the effects of DA at encoding. We suggest that these symmetrical disruptive effects of DA at encoding and retrieval on later retrieval reflect a disruption of an episodic buffer (EB) or episodic register component (ER), rather than a failure of encoding or retrieval operations per se.

The role of the thalamic nuclei in recognition memory accompanied by recall during encoding and retrieval: an fMRI study.

PubMed

Pergola, Giulio; Ranft, Alexander; Mathias, Klaus; Suchan, Boris

2013-07-01

The present functional imaging study aimed at investigating the contribution of the mediodorsal nucleus and the anterior nuclei of the thalamus with their related cortical networks to recognition memory and recall. Eighteen subjects performed associative picture encoding followed by a single item recognition test during the functional magnetic resonance imaging session. After scanning, subjects performed a cued recall test using the formerly recognized pictures as cues. This post-scanning test served to classify recognition trials according to subsequent recall performance. In general, single item recognition accompanied by successful recall of the associations elicited stronger activation in the mediodorsal nucleus of the thalamus and in the prefrontal cortices both during encoding and retrieval compared to recognition without recall. In contrast, the anterior nuclei of the thalamus were selectively active during the retrieval phase of recognition followed by recall. A correlational analysis showed that activation of the anterior thalamus during retrieval as assessed by measuring the percent signal changes predicted lower rates of recognition without recall. These findings show that the thalamus is critical for recognition accompanied by recall, and provide the first evidence of a functional segregation of the thalamic nuclei with respect to the memory retrieval phase. In particular, the mediodorsal thalamic-prefrontal cortical network is activated during successful encoding and retrieval of associations, which suggests a role of this system in recall and recollection. The activity of the anterior thalamic-temporal network selectively during retrieval predicts better memory performances across subjects and this confirms the paramount role of this network in recall and recollection. Copyright © 2013 Elsevier Inc. All rights reserved.

Batching alternatives for Phase I retrieval wastes to be processed in WRAP Module 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mayancsik, B.A.

1994-10-13

During the next two decades, the transuranic (TRU) waste now stored in the 200 Area burial trenches and storage buildings is to be retrieved, processed in the Waste Receiving and Processing (WRAP) Module 1 facility, and shipped to a final disposal facility. The purpose of this document is to identify the criteria that can be used to batch suspect TRU waste, currently in retrievable storage, for processing through the WRAP Module 1 facility. These criteria are then used to generate a batch plan for Phase 1 Retrieval operations, which will retrieve the waste located in Trench 4C-04 of the 200more » West Area burial ground. The reasons for batching wastes for processing in WRAP Module 1 include reducing the exposure of workers and the environment to hazardous material and ionizing radiation; maximizing the efficiency of the retrieval, processing, and disposal processes by reducing costs, time, and space throughout the process; reducing analytical sampling and analysis; and reducing the amount of cleanup and decontamination between process runs. The criteria selected for batching the drums of retrieved waste entering WRAP Module 1 are based on the available records for the wastes sent to storage as well as knowledge of the processes that generated these wastes. The batching criteria identified in this document include the following: waste generator; type of process used to generate or package the waste; physical waste form; content of hazardous/dangerous chemicals in the waste; radiochemical type and quantity of waste; drum weight; and special waste types. These criteria were applied to the waste drums currently stored in Trench 4C-04. At least one batching scheme is shown for each of the criteria listed above.« less

Dorsal hippocampal NMDA receptors mediate the interactive effects of arachidonylcyclopropylamide and MDMA/ecstasy on memory retrieval in rats.

PubMed

Ghaderi, Marzieh; Rezayof, Ameneh; Vousooghi, Nasim; Zarrindast, Mohammad-Reza

2016-04-03

A combination of cannabis and ecstasy may change the cognitive functions more than either drug alone. The present study was designed to investigate the possible involvement of dorsal hippocampal NMDA receptors in the interactive effects of arachidonylcyclopropylamide (ACPA) and ecstasy/MDMA on memory retrieval. Adult male Wistar rats were cannulated into the CA1 regions of the dorsal hippocampus (intra-CA1) and memory retrieval was examined using the step-through type of passive avoidance task. Intra-CA1 microinjection of a selective CB1 receptor agonist, ACPA (0.5-4ng/rat) immediately before the testing phase (pre-test), but not after the training phase (post-training), impaired memory retrieval. In addition, pre-test intra-CA1 microinjection of MDMA (0.5-1μg/rat) dose-dependently decreased step-through latency, indicating an amnesic effect of the drug by itself. Interestingly, pre-test microinjection of a higher dose of MDMA into the CA1 regions significantly improved ACPA-induced memory impairment. Moreover, pre-test intra-CA1 microinjection of a selective NMDA receptor antagonist, D-AP5 (1 and 2μg/rat) inhibited the reversal effect of MDMA on the impairment of memory retrieval induced by ACPA. Pre-test intra-CA1 microinjection of the same doses of D-AP5 had no effect on memory retrieval alone. These findings suggest that ACPA or MDMA consumption can induce memory retrieval impairment, while their co-administration improves this amnesic effect through interacting with hippocampal glutamatergic-NMDA receptor mechanism. Thus, it seems that the tendency to abuse cannabis with ecstasy may be for avoiding cognitive dysfunction. Copyright © 2015. Published by Elsevier Inc.

Optofluidic time-stretch quantitative phase microscopy.

PubMed

Guo, Baoshan; Lei, Cheng; Wu, Yi; Kobayashi, Hirofumi; Ito, Takuro; Yalikun, Yaxiaer; Lee, Sangwook; Isozaki, Akihiro; Li, Ming; Jiang, Yiyue; Yasumoto, Atsushi; Di Carlo, Dino; Tanaka, Yo; Yatomi, Yutaka; Ozeki, Yasuyuki; Goda, Keisuke

2018-03-01

Innovations in optical microscopy have opened new windows onto scientific research, industrial quality control, and medical practice over the last few decades. One of such innovations is optofluidic time-stretch quantitative phase microscopy - an emerging method for high-throughput quantitative phase imaging that builds on the interference between temporally stretched signal and reference pulses by using dispersive properties of light in both spatial and temporal domains in an interferometric configuration on a microfluidic platform. It achieves the continuous acquisition of both intensity and phase images with a high throughput of more than 10,000 particles or cells per second by overcoming speed limitations that exist in conventional quantitative phase imaging methods. Applications enabled by such capabilities are versatile and include characterization of cancer cells and microalgal cultures. In this paper, we review the principles and applications of optofluidic time-stretch quantitative phase microscopy and discuss its future perspective. Copyright © 2017 Elsevier Inc. All rights reserved.

Adaptive optics compensation of orbital angular momentum beams with a modified Gerchberg-Saxton-based phase retrieval algorithm

NASA Astrophysics Data System (ADS)

Chang, Huan; Yin, Xiao-li; Cui, Xiao-zhou; Zhang, Zhi-chao; Ma, Jian-xin; Wu, Guo-hua; Zhang, Li-jia; Xin, Xiang-jun

2017-12-01

Practical orbital angular momentum (OAM)-based free-space optical (FSO) communications commonly experience serious performance degradation and crosstalk due to atmospheric turbulence. In this paper, we propose a wave-front sensorless adaptive optics (WSAO) system with a modified Gerchberg-Saxton (GS)-based phase retrieval algorithm to correct distorted OAM beams. We use the spatial phase perturbation (SPP) GS algorithm with a distorted probe Gaussian beam as the only input. The principle and parameter selections of the algorithm are analyzed, and the performance of the algorithm is discussed. The simulation results show that the proposed adaptive optics (AO) system can significantly compensate for distorted OAM beams in single-channel or multiplexed OAM systems, which provides new insights into adaptive correction systems using OAM beams.

Resolution enhancement in coherent x-ray diffraction imaging by overcoming instrumental noise.

PubMed

Kim, Chan; Kim, Yoonhee; Song, Changyong; Kim, Sang Soo; Kim, Sunam; Kang, Hyon Chol; Hwu, Yeukuang; Tsuei, Ku-Ding; Liang, Keng San; Noh, Do Young

2014-11-17

We report that reference objects, strong scatterers neighboring weak phase objects, enhance the phase retrieval and spatial resolution in coherent x-ray diffraction imaging (CDI). A CDI experiment with Au nano-particles exhibited that the reference objects amplified the signal-to-noise ratio in the diffraction intensity at large diffraction angles, which significantly enhanced the image resolution. The interference between the diffracted x-ray from reference objects and a specimen also improved the retrieval of the phase of the diffraction signal. The enhancement was applied to image NiO nano-particles and a mitochondrion and confirmed in a simulation with a bacteria phantom. We expect that the proposed method will be of great help in imaging weakly scattering soft matters using coherent x-ray sources including x-ray free electron lasers.

Quantitative dispersion microscopy

PubMed Central

Fu, Dan; Choi, Wonshik; Sung, Yongjin; Yaqoob, Zahid; Dasari, Ramachandra R.; Feld, Michael

2010-01-01

Refractive index dispersion is an intrinsic optical property and a useful source of contrast in biological imaging studies. In this report, we present the first dispersion phase imaging of living eukaryotic cells. We have developed quantitative dispersion microscopy based on the principle of quantitative phase microscopy. The dual-wavelength quantitative phase microscope makes phase measurements at 310 nm and 400 nm wavelengths to quantify dispersion (refractive index increment ratio) of live cells. The measured dispersion of living HeLa cells is found to be around 1.088, which agrees well with that measured directly for protein solutions using total internal reflection. This technique, together with the dry mass and morphology measurements provided by quantitative phase microscopy, could prove to be a useful tool for distinguishing different types of biomaterials and studying spatial inhomogeneities of biological samples. PMID:21113234

Quantitative prediction of phase transformations in silicon during nanoindentation

NASA Astrophysics Data System (ADS)

Zhang, Liangchi; Basak, Animesh

2013-08-01

This paper establishes the first quantitative relationship between the phases transformed in silicon and the shape characteristics of nanoindentation curves. Based on an integrated analysis using TEM and unit cell properties of phases, the volumes of the phases emerged in a nanoindentation are formulated as a function of pop-out size and depth of nanoindentation impression. This simple formula enables a fast, accurate and quantitative prediction of the phases in a nanoindentation cycle, which has been impossible before.

Quantitative Absorption and Kinetic Studies of Transient Species Using Gas Phase Optical Calorimetry

NASA Astrophysics Data System (ADS)

Melnik, Dmitry G.

2014-06-01

Quantitative measurements of the absorption cross-sections and reaction rates constants of free radicals by spectroscopic means requires the knowledge of the absolute concentration of the target species. We have demonstrated earlier that such information can be retrieved from absorption measurements of the well-known ``reporter" molecule, co-produced in radical synthesis. This method is limited to photochemical protocols allowing for production of ``reporters" stochiometrically with the target species. This limitation can be overcome by use of the optical calorimetry (OC) which measures heat signatures of a photochemical protocol. These heat signatures are directly related to the amount of species produced and the thermochemical data of the reactants and stable products whose accuracy is usually substantially higher than that of the absorption data for prospective ``reporters". The implementation of the OC method presented in this talk is based on the measurements of the frequency shift of the resonances due to the change in the optical density of the reactiove sample within a Fabry-Perot cavity caused by deposition of heat from the absorbed photolysis beam and subsequent chemical reactions. Preliminary results will be presented and future development of this experimental technique will be discussed. D. Melnik, R. Chhantyal-Pun and T. A. Miller, J. Phys. Chem. A, 114, 11583, (2010)

The influence of learning methods on collaboration: prior repeated retrieval enhances retrieval organization, abolishes collaborative inhibition, and promotes post-collaborative memory.

PubMed

Congleton, Adam R; Rajaram, Suparna

2011-11-01

Research on collaborative memory has unveiled the counterintuitive yet robust phenomenon that collaboration impairs group recall. A candidate explanation for this collaborative inhibition effect is the disruption of people's idiosyncratic retrieval strategies during collaboration, and it is hypothesized that employing methods that improve one's organization protects against retrieval disruption. Here it is investigated how one's learning method during the study phase--defined as either repeatedly studying or repeatedly retrieving information--influences retrieval organization and what effects this has on collaborative recall and post-collaborative individual recall. Results show that repeated retrieval consistently eliminated collaborative inhibition. This enabled participants to gain the most from re-exposure to materials recalled by their partners that they themselves did not recall and led to improvements in their individual memory following collaboration. This repeated retrieval advantage stemmed from the preferential manner in which this learning method strengthened retrieval organization. Findings are also discussed that reveal a relationship between retrieval organization and the interaction observed between learning method and short versus long delay seen in the testing effect literature. Finally, results show that the elusive benefits of cross-cuing during collaboration may be best detected with a longer study-test delay. Together, these findings illuminate when and how collaboration can enhance memory.

Comparison of different phase retrieval algorithms

NASA Astrophysics Data System (ADS)

Kaufmann, Rolf; Plamondon, Mathieu; Hofmann, Jürgen; Neels, Antonia

2017-09-01

X-ray phase contrast imaging is attracting more and more interest. Since the phase cannot be measured directly an indirect method using e.g. a grating interferometer has to be applied. This contribution compares three different approaches to calculate the phase from Talbot-Lau interferometer measurements using a phase-stepping approach. Besides the usually applied Fourier coefficient method also a linear fitting technique and Taylor series expansion method are applied and compared.

Subsurface imaging and cell refractometry using quantitative phase/ shear-force feedback microscopy

NASA Astrophysics Data System (ADS)

Edward, Kert; Farahi, Faramarz

2009-10-01

Over the last few years, several novel quantitative phase imaging techniques have been developed for the study of biological cells. However, many of these techniques are encumbered by inherent limitations including 2π phase ambiguities and diffraction limited spatial resolution. In addition, subsurface information in the phase data is not exploited. We hereby present a novel quantitative phase imaging system without 2 π ambiguities, which also allows for subsurface imaging and cell refractometry studies. This is accomplished by utilizing simultaneously obtained shear-force topography information. We will demonstrate how the quantitative phase and topography data can be used for subsurface and cell refractometry analysis and will present results for a fabricated structure and a malaria infected red blood cell.

Validation of high-resolution MAIAC aerosol product over South America

NASA Astrophysics Data System (ADS)

Martins, V. S.; Lyapustin, A.; de Carvalho, L. A. S.; Barbosa, C. C. F.; Novo, E. M. L. M.

2017-07-01

Multiangle Implementation of Atmospheric Correction (MAIAC) is a new Moderate Resolution Imaging Spectroradiometer (MODIS) algorithm that combines time series approach and image processing to derive surface reflectance and atmosphere products, such as aerosol optical depth (AOD) and columnar water vapor (CWV). The quality assessment of MAIAC AOD at 1 km resolution is still lacking across South America. In the present study, critical assessment of MAIAC AOD550 was performed using ground-truth data from 19 Aerosol Robotic Network (AERONET) sites over South America. Additionally, we validated the MAIAC CWV retrievals using the same AERONET sites. In general, MAIAC AOD Terra/Aqua retrievals show high agreement with ground-based measurements, with a correlation coefficient (R) close to unity (RTerra:0.956 and RAqua: 0.949). MAIAC accuracy depends on the surface properties and comparisons revealed high confidence retrievals over cropland, forest, savanna, and grassland covers, where more than 2/3 ( 66%) of retrievals are within the expected error (EE = ±(0.05 + 0.05 × AOD)) and R exceeding 0.86. However, AOD retrievals over bright surfaces show lower correlation than those over vegetated areas. Both MAIAC Terra and Aqua retrievals are similarly comparable to AERONET AOD over the MODIS lifetime (small bias offset 0.006). Additionally, MAIAC CWV presents quantitative information with R 0.97 and more than 70% of retrievals within error (±15%). Nonetheless, the time series validation shows an upward bias trend in CWV Terra retrievals and systematic negative bias for CWV Aqua. These results contribute to a comprehensive evaluation of MAIAC AOD retrievals as a new atmospheric product for future aerosol studies over South America.

Testing enhances both encoding and retrieval for both tested and untested items.

PubMed

Cho, Kit W; Neely, James H; Crocco, Stephanie; Vitrano, Deana

2017-07-01

In forward testing effects, taking a test enhances memory for subsequently studied material. These effects have been observed for previously studied and tested items, a potentially item-specific testing effect, and newly studied untested items, a purely generalized testing effect. We directly compared item-specific and generalized forward testing effects using procedures to separate testing benefits due to encoding versus retrieval. Participants studied two lists of Swahili-English word pairs, with the second study list containing "new" pairs intermixed with the previously studied "old" pairs. Participants completed a review phase in which they took a cued-recall test on only the "old" pairs or restudied them. In Experiments 1a, 1b, and 2, the review phase was given either before or after the second study list. Testing benefited memory to the same degree for both "new" and "old" pairs, suggesting that there were no pair-specific benefits of testing. The larger benefit from testing when review was given before rather than after the second study list suggests that the memory enhancement was due to both testing-enhanced encoding and testing-enhanced retrieval. To better equate generalized testing effects for "new" and "old" pairs, Experiment 3 intermixed them in the review phase. A statistically significant pair-specific testing effect for "old" items was now observed. Overall, these results show that forward testing effects are due to both testing-enhanced encoding and retrieval effects and that direct, pair-specific forward testing benefits are considerably smaller than indirect, generalized forward testing benefits.

Soft-tissue and phase-contrast imaging at the Swiss Light Source

NASA Astrophysics Data System (ADS)

Schneider, Philipp; Mohan, Nishant; Stampanoni, Marco; Muller, Ralph

2004-05-01

Recent results show that bone vasculature is a major contributor to local tissue porosity, and therefore can be directly linked to the mechanical properties of bone tissue. With the advent of third generation synchrotron radiation (SR) sources, micro-computed tomography (μCT) with resolutions in the order of 1 μm and better has become feasible. This technique has been employed frequently to analyze trabecular architecture and local bone tissue properties, i.e. the hard or mineralized bone tissue. Nevertheless, less is known about the soft tissues in bone, mainly due to inadequate imaging capabilities. Here, we discuss three different methods and applications to visualize soft tissues. The first approach is referred to as negative imaging. In this case the material around the soft tissue provides the absorption contrast necessary for X-ray based tomography. Bone vasculature from two different mouse strains was investigated and compared qualitatively. Differences were observed in terms of local vessel number and vessel orientation. The second technique represents corrosion casting, which is principally adapted for imaging of vascular systems. The technique of corrosion casting has already been applied successfully at the Swiss Light Source. Using the technology we were able to show that pathological features reminiscent of Alzheimer"s disease could be distinguished in the brain vasculature of APP transgenic mice. The third technique discussed here is phase contrast imaging exploiting the high degree of coherence of third generation synchrotron light sources, which provide the necessary physical conditions for phase contrast. The in-line approach followed here for phase contrast retrieval is a modification of the Gerchberg-Saxton-Fienup type. Several measurements and theoretical thoughts concerning phase contrast imaging are presented, including mathematical phase retrieval. Although up-to-now only phase images have been computed, the approach is now ready to retrieve the phase for a large number of angular positions of the specimen allowing application of holotomography, which is the three-dimensional reconstruction of phase images.

Skin Parameter Map Retrieval from a Dedicated Multispectral Imaging System Applied to Dermatology/Cosmetology

PubMed Central

2013-01-01

In vivo quantitative assessment of skin lesions is an important step in the evaluation of skin condition. An objective measurement device can help as a valuable tool for skin analysis. We propose an explorative new multispectral camera specifically developed for dermatology/cosmetology applications. The multispectral imaging system provides images of skin reflectance at different wavebands covering visible and near-infrared domain. It is coupled with a neural network-based algorithm for the reconstruction of reflectance cube of cutaneous data. This cube contains only skin optical reflectance spectrum in each pixel of the bidimensional spatial information. The reflectance cube is analyzed by an algorithm based on a Kubelka-Munk model combined with evolutionary algorithm. The technique allows quantitative measure of cutaneous tissue and retrieves five skin parameter maps: melanin concentration, epidermis/dermis thickness, haemoglobin concentration, and the oxygenated hemoglobin. The results retrieved on healthy participants by the algorithm are in good accordance with the data from the literature. The usefulness of the developed technique was proved during two experiments: a clinical study based on vitiligo and melasma skin lesions and a skin oxygenation experiment (induced ischemia) with healthy participant where normal tissues are recorded at normal state and when temporary ischemia is induced. PMID:24159326

A method of solving tilt illumination for multiple distance phase retrieval

NASA Astrophysics Data System (ADS)

Guo, Cheng; Li, Qiang; Tan, Jiubin; Liu, Shutian; Liu, Zhengjun

2018-07-01

Multiple distance phase retrieval is a technique of using a series of intensity patterns to reconstruct a complex-valued image of object. However, tilt illumination originating from the off-axis displacement of incident light significantly impairs its imaging quality. To eliminate this affection, we use cross-correlation calibration to estimate oblique angle of incident light and a Fourier-based strategy to correct tilted illumination effect. Compared to other methods, binary and biological object are both stably reconstructed in simulation and experiment. This work provides a simple but beneficial method to solve the problem of tilt illumination for lens-free multi-distance system.

Topography of hidden objects using THz digital holography with multi-beam interferences.

PubMed

Valzania, Lorenzo; Zolliker, Peter; Hack, Erwin

2017-05-15

We present a method for the separation of the signal scattered from an object hidden behind a THz-transparent sample in the framework of THz digital holography in reflection. It combines three images of different interference patterns to retrieve the amplitude and phase distribution of the object beam. Comparison of simulated with experimental images obtained from a metallic resolution target behind a Teflon plate demonstrates that the interference patterns can be described in the simple form of three-beam interference. Holographic reconstructions after the application of the method show a considerable improvement compared to standard reconstructions exclusively based on Fourier transform phase retrieval.

Multilevel recording of complex amplitude data pages in a holographic data storage system using digital holography.

PubMed

Nobukawa, Teruyoshi; Nomura, Takanori

2016-09-05

A holographic data storage system using digital holography is proposed to record and retrieve multilevel complex amplitude data pages. Digital holographic techniques are capable of modulating and detecting complex amplitude distribution using current electronic devices. These techniques allow the development of a simple, compact, and stable holographic storage system that mainly consists of a single phase-only spatial light modulator and an image sensor. As a proof-of-principle experiment, complex amplitude data pages with binary amplitude and four-level phase are recorded and retrieved. Experimental results show the feasibility of the proposed holographic data storage system.

Active sensor synergy for arctic cloud microphysics

NASA Astrophysics Data System (ADS)

Sato, Kaori; Okamoto, Hajime; Katagiri, Shuichiro; Shiobara, Masataka; Yabuki, Masanori; Takano, Toshiaki

2018-04-01

In this study, we focus on the retrieval of liquid and ice-phase cloud microphysics from spaceborne and ground-based lidar-cloud radar synergy. As an application of the cloud retrieval algorithm developed for the EarthCARE satellite mission (JAXA-ESA) [1], the derived statistics of cloud microphysical properties in high latitudes and their relation to the Arctic climate are investigated.

Incidental Retrieval of Emotional Contexts in Post-Traumatic Stress Disorder and Depression: An fMRI Study

ERIC Educational Resources Information Center

Whalley, Matthew G.; Rugg, Michael D.; Smith, Adam P. R.; Dolan, Raymond J.; Brewin, Chris R.

2009-01-01

In the present study, we used fMRI to assess patients suffering from post-traumatic stress disorder (PTSD) or depression, and trauma-exposed controls, during an episodic memory retrieval task that included non-trauma-related emotional information. In the study phase of the task neutral pictures were presented in emotional or neutral contexts.…

Invited Article: Mask-modulated lensless imaging with multi-angle illuminations

NASA Astrophysics Data System (ADS)

Zhang, Zibang; Zhou, You; Jiang, Shaowei; Guo, Kaikai; Hoshino, Kazunori; Zhong, Jingang; Suo, Jinli; Dai, Qionghai; Zheng, Guoan

2018-06-01

The use of multiple diverse measurements can make lensless phase retrieval more robust. Conventional diversity functions include aperture diversity, wavelength diversity, translational diversity, and defocus diversity. Here we discuss a lensless imaging scheme that employs multiple spherical-wave illuminations from a light-emitting diode array as diversity functions. In this scheme, we place a binary mask between the sample and the detector for imposing support constraints for the phase retrieval process. This support constraint enforces the light field to be zero at certain locations and is similar to the aperture constraint in Fourier ptychographic microscopy. We use a self-calibration algorithm to correct the misalignment of the binary mask. The efficacy of the proposed scheme is first demonstrated by simulations where we evaluate the reconstruction quality using mean square error and structural similarity index. The scheme is then experimentally tested by recovering images of a resolution target and biological samples. The proposed scheme may provide new insights for developing compact and large field-of-view lensless imaging platforms. The use of the binary mask can also be combined with other diversity functions for better constraining the phase retrieval solution space. We provide the open-source implementation code for the broad research community.

Phase retrieval via incremental truncated amplitude flow algorithm

NASA Astrophysics Data System (ADS)

Zhang, Quanbing; Wang, Zhifa; Wang, Linjie; Cheng, Shichao

2017-10-01

This paper considers the phase retrieval problem of recovering the unknown signal from the given quadratic measurements. A phase retrieval algorithm based on Incremental Truncated Amplitude Flow (ITAF) which combines the ITWF algorithm and the TAF algorithm is proposed. The proposed ITAF algorithm enhances the initialization by performing both of the truncation methods used in ITWF and TAF respectively, and improves the performance in the gradient stage by applying the incremental method proposed in ITWF to the loop stage of TAF. Moreover, the original sampling vector and measurements are preprocessed before initialization according to the variance of the sensing matrix. Simulation experiments verified the feasibility and validity of the proposed ITAF algorithm. The experimental results show that it can obtain higher success rate and faster convergence speed compared with other algorithms. Especially, for the noiseless random Gaussian signals, ITAF can recover any real-valued signal accurately from the magnitude measurements whose number is about 2.5 times of the signal length, which is close to the theoretic limit (about 2 times of the signal length). And it usually converges to the optimal solution within 20 iterations which is much less than the state-of-the-art algorithms.

Coadding Techniques for Image-based Wavefront Sensing for Segmented-mirror Telescopes

NASA Technical Reports Server (NTRS)

Smith, Scott; Aronstein, David; Dean, Bruce; Acton, Scott

2007-01-01

Image-based wavefront sensing algorithms are being used to characterize optical performance for a variety of current and planned astronomical telescopes. Phase retrieval recovers the optical wavefront that correlates to a series of diversity-defocused point-spread functions (PSFs), where multiple frames can be acquired at each defocus setting. Multiple frames of data can be coadded in different ways; two extremes are in "image-plane space," to average the frames for each defocused PSF and use phase retrieval once on the averaged images, or in "pupil-plane space," to use phase retrieval on every set of PSFs individually and average the resulting wavefronts. The choice of coadd methodology is particularly noteworthy for segmented-mirror telescopes that are subject to noise that causes uncorrelated motions between groups of segments. Using data collected on and simulations of the James Webb Space Telescope Testbed Telescope (TBT) commissioned at Ball Aerospace, we show how different sources of noise (uncorrelated segment jitter, turbulence, and common-mode noise) and different parts of the optical wavefront, segment and global aberrations, contribute to choosing the coadd method. Of particular interest, segment piston is more accurately recovered in "image-plane space" coadding, while segment tip/tilt is recovered in "pupil-plane space" coadding.

Sampling and Reconstruction of the Pupil and Electric Field for Phase Retrieval

NASA Technical Reports Server (NTRS)

Dean, Bruce; Smith, Jeffrey; Aronstein, David

2012-01-01

This technology is based on sampling considerations for a band-limited function, which has application to optical estimation generally, and to phase retrieval specifically. The analysis begins with the observation that the Fourier transform of an optical aperture function (pupil) can be implemented with minimal aliasing for Q values down to Q = 1. The sampling ratio, Q, is defined as the ratio of the sampling frequency to the band-limited cut-off frequency. The analytical results are given using a 1-d aperture function, and with the electric field defined by the band-limited sinc(x) function. Perfect reconstruction of the Fourier transform (electric field) is derived using the Whittaker-Shannon sampling theorem for 1

Answering the Call for Model-Relevant Observations of Aerosols and Clouds

NASA Technical Reports Server (NTRS)

Redemann, J.; Shinozuka, Y.; Kacenelenbogen, M.; Segal-Rozenhaimer, M.; LeBlanc, S.; Vaughan, M.; Stier, P.; Schutgens, N.

2017-01-01

We describe a technique for combining multiple A-Train aerosol data sets, namely MODIS spectral AOD (aerosol optical depth), OMI AAOD (absorption aerosol optical depth) and CALIOP aerosol backscatter retrievals (hereafter referred to as MOC retrievals) to estimate full spectral sets of aerosol radiative properties, and ultimately to calculate the 3-D distribution of direct aerosol radiative effects (DARE). We present MOC results using almost two years of data collected in 2007 and 2008, and show comparisons of the aerosol radiative property estimates to collocated AERONET retrievals. We compare the spatio-temporal distribution of the MOC retrievals and MOC-based calculations of seasonal clear-sky DARE to values derived from four models that participated in the Phase II AeroCom model intercomparison initiative. Comparisons of seasonal aerosol property to AeroCom Phase II results show generally good agreement best agreement with forcing results at TOA is found with GMI-MerraV3.We discuss the challenges in making observations that really address deficiencies in models, with some of the more relevant aspects being representativeness of the observations for climatological states, and whether a given model-measurement difference addresses a sampling or a model error.

Local Time Variation of Water Vapor on Mars using TES Aerobraking Spectra

NASA Astrophysics Data System (ADS)

AlShamsi, M. R.; AlJanaahi, A. A.; Smith, M. D.; Altunaiji, E. S.; Edwards, C. S.

2016-12-01

During the Mars Global Surveyor (MGS) aerobraking phase, the spacecraft was in a large elliptical orbit that enabled the Thermal Emission Spectrometer (TES) instrument to sample many local times of Mars. The observed TES aerobraking spectra during that phase cover the time range between Mars Year 23, Ls=180° and Mars Year 24, Ls=30°. These TES aerobraking spectra have never been analyzed to study local time variations on Mars. Through radiative transfer modeling of the spectra, surface and atmospheric temperature, dust and water ice optical depth, and water vapor were retrieved. Specifically, the water vapor retrievals during aerobraking have similar seasonal and latitudinal trends to those in other Mars years observed by TES. These retrievals show somewhat higher water vapor during the morning hours (09:00-12:00) than in the afternoon (12:00-17:00) during southern summer (Ls=270°-330°) and little variation as a function of local time for southern fall (Ls=0°-30°). These retrievals show water vapor has a positive correlation with surface pressure (or negative correlation with altitude) indicating that water vapor is mixed in the lowest 10-20 km.

Quantitative NTCP Pharmacophore and Lack of Association between DILI and NTCP Inhibition

PubMed Central

Dong, Zhongqi; Ekins, Sean; Polli, James E.

2014-01-01

The human sodium taurocholate cotransporting polypeptide (NTCP) is a hepatic bile acid transporter. Inhibition of NTCP uptake may potentially also prevent hepatitis B virus (HBV) infection. The first objective was to develop a quantitative pharmacophore for NTCP inhibition. Recent studies showed that hepatotoxic drugs could inhibit bile acid uptake into hepatocytes, without inhibiting canalicular efflux, and cause bile acid elevation in plasma. Hence, a second objective was to examine whether NTCP inhibition is associated with drug induced liver injury (DILI). Twenty-seven drugs from our previous study were used as the training set to develop a quantitative pharmacophore. From secondary screening from a drug database, six retrieved drugs and three drugs not retrieved by the model were tested for NTCP inhibition. Tertiary screening involved drugs known to cause DILI and not cause DILI. Overall, ninety-four drugs were assessed for hepatotoxicity and were assessed relative to NTCP inhibition. The quantitative pharmacophore possessed one hydrogen bond acceptor, one hydrogen bond donor, a hydrophobic feature, and excluded volumes. From 94 drugs, NTCP inhibitors and non-inhibitors were approximately equally distributed across the drugs of most DILI concern, less DILI concern, and no DILI concern, indicating no relationship between NTCP inhibition and DILI risk. Hence, an approach to treat HBV via NTCP inhibition is not expected to be associated with DILI. PMID:25220493

Quantitative NTCP pharmacophore and lack of association between DILI and NTCP Inhibition.

PubMed

Dong, Zhongqi; Ekins, Sean; Polli, James E

2015-01-23

The human sodium taurocholate cotransporting polypeptide (NTCP) is a hepatic bile acid transporter. Inhibition of NTCP uptake may potentially also prevent hepatitis B virus (HBV) infection. The first objective was to develop a quantitative pharmacophore for NTCP inhibition. Recent studies showed that hepatotoxic drugs could inhibit bile acid uptake into hepatocytes, without inhibiting canalicular efflux, and cause bile acid elevation in plasma. Hence, a second objective was to examine whether NTCP inhibition is associated with drug induced liver injury (DILI). Twenty-seven drugs from our previous study were used as the training set to develop a quantitative pharmacophore. From secondary screening from a drug database, six retrieved drugs and three drugs not retrieved by the model were tested for NTCP inhibition. Tertiary screening involved drugs known to cause DILI and not cause DILI. Overall, ninety-four drugs were assessed for hepatotoxicity and were assessed relative to NTCP inhibition. The quantitative pharmacophore possessed one hydrogen bond acceptor, one hydrogen bond donor, a hydrophobic feature, and excluded volumes. From 94 drugs, NTCP inhibitors and non-inhibitors were approximately equally distributed across the drugs of most DILI concern, less DILI concern, and no DILI concern, indicating no relationship between NTCP inhibition and DILI risk. Hence, an approach to treat HBV via NTCP inhibition is not expected to be associated with DILI. Copyright © 2014 Elsevier B.V. All rights reserved.

Reward modulation of hippocampal subfield activation during successful associative encoding and retrieval

PubMed Central

Wolosin, Sasha M.; Zeithamova, Dagmar; Preston, Alison R.

2012-01-01

Emerging evidence suggests that motivation enhances episodic memory formation through interactions between medial temporal lobe (MTL) structures and dopaminergic midbrain. In addition, recent theories propose that motivation specifically facilitates hippocampal associative binding processes, resulting in more detailed memories that are readily reinstated from partial input. Here, we used high-resolution functional magnetic resonance imaging to determine how motivation influences associative encoding and retrieval processes within human MTL subregions and dopaminergic midbrain. Participants intentionally encoded object associations under varying conditions of reward and performed a retrieval task during which studied associations were cued from partial input. Behaviorally, cued recall performance was superior for high-value relative to low-value associations; however, participants differed in the degree to which rewards influenced memory. The magnitude of behavioral reward modulation was associated with reward-related activation changes in dentate gyrus/CA2,3 during encoding and enhanced functional connectivity between dentate gyrus/CA2,3 and dopaminergic midbrain during both the encoding and retrieval phases of the task. These findings suggests that within the hippocampus, reward-based motivation specifically enhances dentate gyrus/CA2,3 associative encoding mechanisms through interactions with dopaminergic midbrain. Furthermore, within parahippocampal cortex and dopaminergic midbrain regions, activation associated with successful memory formation was modulated by reward across the group. During the retrieval phase, we also observed enhanced activation in hippocampus and dopaminergic midbrain for high-value associations that occurred in the absence of any explicit cues to reward. Collectively, these findings shed light on fundamental mechanisms through which reward impacts associative memory formation and retrieval through facilitation of MTL and VTA/SN processing. PMID:22524296

Phase calibration target for quantitative phase imaging with ptychography.

PubMed

Godden, T M; Muñiz-Piniella, A; Claverley, J D; Yacoot, A; Humphry, M J

2016-04-04

Quantitative phase imaging (QPI) utilizes refractive index and thickness variations that lead to optical phase shifts. This gives contrast to images of transparent objects. In quantitative biology, phase images are used to accurately segment cells and calculate properties such as dry mass, volume and proliferation rate. The fidelity of the measured phase shifts is of critical importance in this field. However to date, there has been no standardized method for characterizing the performance of phase imaging systems. Consequently, there is an increasing need for protocols to test the performance of phase imaging systems using well-defined phase calibration and resolution targets. In this work, we present a candidate for a standardized phase resolution target, and measurement protocol for the determination of the transfer of spatial frequencies, and sensitivity of a phase imaging system. The target has been carefully designed to contain well-defined depth variations over a broadband range of spatial frequencies. In order to demonstrate the utility of the target, we measure quantitative phase images on a ptychographic microscope, and compare the measured optical phase shifts with Atomic Force Microscopy (AFM) topography maps and surface profile measurements from coherence scanning interferometry. The results show that ptychography has fully quantitative nanometer sensitivity in optical path differences over a broadband range of spatial frequencies for feature sizes ranging from micrometers to hundreds of micrometers.

Retrieval analysis of ceramic-coated metal-on-polyethylene total hip replacements.

PubMed

Khatkar, Harman; Hothi, Harry; de Villiers, Danielle; Lausmann, Christian; Kendoff, Daniel; Gehrke, Thorsten; Skinner, John; Hart, Alister

2017-06-01

Ceramic coatings have been used in metal-on-polyethylene (MOP) hips to reduce the risk of wear and also infection; the clinical efficacy of this remains unclear. This retrieval study sought to better understand the performance of coated bearing surfaces. Forty-three coated MOP components were analysed post-retrieval for evidence of coating loss and gross polyethylene wear. Coating loss was graded using a visual semi-quantitative protocol. Evidence of gross polyethylene wear was determined by radiographic analysis and visual inspection of the retrieved implants. All components with gross polyethylene wear (n = 10) were revised due to a malfunctioning acetabular component; 35 % (n = 15) of implants exhibited visible coating loss and the incidence of polyethylene wear in samples with coating loss was 54 %, significantly (p = 0.02) elevated compared to samples with intact coatings (14 %). In this study we found evidence of coating loss on metal femoral heads which was associated with increased wear of the corresponding polyethylene acetabular cups.

Modis Collection 6 Shortwave-Derived Cloud Phase Classification Algorithm and Comparisons with CALIOP

NASA Technical Reports Server (NTRS)

Marchant, Benjamin; Platnick, Steven; Meyer, Kerry; Arnold, George Thomas; Riedi, Jerome

2016-01-01

Cloud thermodynamic phase (e.g., ice, liquid) classification is an important first step for cloud retrievals from passive sensors such as MODIS (Moderate-Resolution Imaging Spectroradiometer). Because ice and liquid phase clouds have very different scattering and absorbing properties, an incorrect cloud phase decision can lead to substantial errors in the cloud optical and microphysical property products such as cloud optical thickness or effective particle radius. Furthermore, it is well established that ice and liquid clouds have different impacts on the Earth's energy budget and hydrological cycle, thus accurately monitoring the spatial and temporal distribution of these clouds is of continued importance. For MODIS Collection 6 (C6), the shortwave-derived cloud thermodynamic phase algorithm used by the optical and microphysical property retrievals has been completely rewritten to improve the phase discrimination skill for a variety of cloudy scenes (e.g., thin/thick clouds, over ocean/land/desert/snow/ice surface, etc). To evaluate the performance of the C6 cloud phase algorithm, extensive granule-level and global comparisons have been conducted against the heritage C5 algorithm and CALIOP. A wholesale improvement is seen for C6 compared to C5.

The Ozone Mapping and Profiler Suite (OMPS) Limb Profiler (LP) Version 1 aerosol extinction retrieval algorithm: theoretical basis

NASA Astrophysics Data System (ADS)

Loughman, Robert; Bhartia, Pawan K.; Chen, Zhong; Xu, Philippe; Nyaku, Ernest; Taha, Ghassan

2018-05-01

The theoretical basis of the Ozone Mapping and Profiler Suite (OMPS) Limb Profiler (LP) Version 1 aerosol extinction retrieval algorithm is presented. The algorithm uses an assumed bimodal lognormal aerosol size distribution to retrieve aerosol extinction profiles at 675 nm from OMPS LP radiance measurements. A first-guess aerosol extinction profile is updated by iteration using the Chahine nonlinear relaxation method, based on comparisons between the measured radiance profile at 675 nm and the radiance profile calculated by the Gauss-Seidel limb-scattering (GSLS) radiative transfer model for a spherical-shell atmosphere. This algorithm is discussed in the context of previous limb-scattering aerosol extinction retrieval algorithms, and the most significant error sources are enumerated. The retrieval algorithm is limited primarily by uncertainty about the aerosol phase function. Horizontal variations in aerosol extinction, which violate the spherical-shell atmosphere assumed in the version 1 algorithm, may also limit the quality of the retrieved aerosol extinction profiles significantly.

Incidental retrieval-induced forgetting of location information.

PubMed

Gómez-Ariza, Carlos J; Fernandez, Angel; Bajo, M Teresa

2012-06-01

Retrieval-induced forgetting (RIF) has been studied with different types of tests and materials. However, RIF has always been tested on the items' central features, and there is no information on whether inhibition also extends to peripheral features of the events in which the items are embedded. In two experiments, we specifically tested the presence of RIF in a task in which recall of peripheral information was required. After a standard retrieval practice task oriented to item identity, participants were cued with colors (Exp. 1) or with the items themselves (Exp. 2) and asked to recall the screen locations where the items had been displayed during the study phase. RIF for locations was observed after retrieval practice, an effect that was not present when participants were asked to read instead of retrieving the items. Our findings provide evidence that peripheral location information associated with an item during study can be also inhibited when the retrieval conditions promote the inhibition of more central, item identity information.

A retrieval-based approach to eliminating hindsight bias.

PubMed

Van Boekel, Martin; Varma, Keisha; Varma, Sashank

2017-03-01

Individuals exhibit hindsight bias when they are unable to recall their original responses to novel questions after correct answers are provided to them. Prior studies have eliminated hindsight bias by modifying the conditions under which original judgments or correct answers are encoded. Here, we explored whether hindsight bias can be eliminated by manipulating the conditions that hold at retrieval. Our retrieval-based approach predicts that if the conditions at retrieval enable sufficient discrimination of memory representations of original judgments from memory representations of correct answers, then hindsight bias will be reduced or eliminated. Experiment 1 used the standard memory design to replicate the hindsight bias effect in middle-school students. Experiments 2 and 3 modified the retrieval phase of this design, instructing participants beforehand that they would be recalling both their original judgments and the correct answers. As predicted, this enabled participants to form compound retrieval cues that discriminated original judgment traces from correct answer traces, and eliminated hindsight bias. Experiment 4 found that when participants were not instructed beforehand that they would be making both recalls, they did not form discriminating retrieval cues, and hindsight bias returned. These experiments delineate the retrieval conditions that produce-and fail to produce-hindsight bias.

Quantitative phase-contrast digital holographic microscopy for cell dynamic evaluation

NASA Astrophysics Data System (ADS)

Yu, Lingfeng; Mohanty, Samarendra; Berns, Michael W.; Chen, Zhongping

2009-02-01

The laser microbeam uses lasers to alter and/or to ablate intracellular organelles and cellular and tissue samples, and, today, has become an important tool for cell biologists to study the molecular mechanism of complex biological systems by removing individual cells or sub-cellular organelles. However, absolute quantitation of the localized alteration/damage to transparent phase objects, such as the cell membrane or chromosomes, was not possible using conventional phase-contrast or differential interference contrast microscopy. We report the development of phase-contrast digital holographic microscopy for quantitative evaluation of cell dynamic changes in real time during laser microsurgery. Quantitative phase images are recorded during the process of laser microsurgery and thus, the dynamic change in phase can be continuously evaluated. Out-of-focus organelles are re-focused by numerical reconstruction algorithms.

The astronaut and the banana peel: An EVA retriever scenario

NASA Technical Reports Server (NTRS)

Shapiro, Daniel G.

1989-01-01

To prepare for the problem of accidents in Space Station activities, the Extravehicular Activity Retriever (EVAR) robot is being constructed, whose purpose is to retrieve astronauts and tools that float free of the Space Station. Advanced Decision Systems is at the beginning of a project to develop research software capable of guiding EVAR through the retrieval process. This involves addressing problems in machine vision, dexterous manipulation, real time construction of programs via speech input, and reactive execution of plans despite the mishaps and unexpected conditions that arise in uncontrolled domains. The problem analysis phase of this work is presented. An EVAR scenario is used to elucidate major domain and technical problems. An overview of the technical approach to prototyping an EVAR system is also presented.

Towards better understanding of high-mountain cryosphere changes using GPM data: A Joint Snowfall and Snow-cover Passive Microwave Retrieval Algorithm

NASA Astrophysics Data System (ADS)

Ebtehaj, A.; Foufoula-Georgiou, E.

2016-12-01

Scientific evidence suggests that the duration and frequency of snowfall and the extent of snow cover are rapidly declining under global warming. Both precipitation and snow cover scatter the upwelling surface microwave emission and decrease the observed high-frequency brightness temperatures. The mixture of these two scattering signals is amongst the largest sources of ambiguities and errors in passive microwave retrievals of both precipitation and snow-cover. The dual frequency radar and the high-frequency radiometer on board the GPM satellite provide a unique opportunity to improve passive retrievals of precipitation and snow-cover physical properties and fill the gaps in our understating of their variability in view of climate change. Recently, a new Bayesian rainfall retrieval algorithm (called ShARP) was developed using modern approximation methods and shown to yield improvements against other algorithms in retrieval of rainfall over radiometrically complex land surfaces. However, ShARP uses a large database of input rainfall and output brightness temperatures, which might be undersampled. Furthermore, it is not capable to discriminate between solid and liquid phase of precipitation and specifically discriminate the background snow-cover emission and its contamination effects on the retrievals. We address these problems by extending it to a new Bayesian land-atmosphere retrieval framework (ShARP-L) that allows joint retrievals of atmospheric constituents and land surface physical properties. Using modern sparse approximation techniques, the database is reduced to atomic microwave signatures in a family of compact class consistent dictionaries. These dictionaries can efficiently represent the entire database and allow us to discriminate between different land-atmosphere states. First the algorithm makes use of the dictionaries to detect the phase of the precipitation and type of the land-cover and then it estimates the physical properties of precipitation and snow cover using an extended version of the Dantzig Selector, which is robust to non-Gaussian and correlated geophysical noise. Promising results are presented in retrievals of snowfall and snow-cover over coastal orographic features of North America's Coast Range and South America's Andes.

The Afar rift zone deformation dynamics retrieved through phase and amplitude SAR data

NASA Astrophysics Data System (ADS)

Casu, F.; Pagli, C.; Paglia, L.; Wang, H.; Wright, T. J.; Lanari, R.

2011-12-01

The Dabbahu rift segment of the Afar depression has been active since 2005 when a 2.5 km3 dyke intrusion and hundreds of earthquakes marked the onset a rifting episode which continues to date. Since 2003, the Afar depression has been repeatedly imaged by the ENVISAT satellite, generating a large SAR archive which allow us to study the ongoing deformation processes and the dynamics of magma movements. We combine sets of small baseline interferograms through the advanced DInSAR algorithm referred to as Small BAseline Subset (SBAS), and we generate both ground deformation maps and time series along the satellite Line-Of-Sight (LOS), with accuracies on the order of 5 mm. The main limitation of DInSAR applications is that large and rapid deformations, such as those caused by dyke intrusions and eruptions in Afar, cannot be fully measured. The phase information often degrades and some areas of the interferograms are affected by high fringe rates, leading to difficulties in the phase unwrapping, and/or to complete loss of coherence due to significant misregistration errors. This limitation can be overcome by exploiting the SAR image amplitude information instead of the phase, and by calculating the Pixel-Offset (PO) field of a given SAR image pair, for both range and azimuth directions. Moreover, after computing the POs for each image pair, it is possible to combine them, following the same rationale of the SBAS technique, to finally retrieve the offset-based deformation time series. Such technique, named PO-SBAS, permits to retrieve the deformation field in areas affected by very large displacements at an accuracy that, for ENVISAT data, correspond to 30cm and 15 cm for the range and azimuth, respectively. In this work, we study the Afar rift region deformations by using both the phase and amplitude information of several sets of SAR images acquired from ascending and descending ENVISAT tracks. In particular, we use the phase information to construct dense and accurate deformation maps and time series in areas not affected by large displacements. While in areas where the deformation gradient causes loss of coherence, we retrieve the displacement field through the amplitude information. This approach allows us to obtain a spatially detailed deformation map of the study area. In addition, by combining ascending and descending data we reconstruct the vertical and East-West components of deformation field. Furthermore, in areas affected by large deformations, we can also retrieve the full 3D deformation field, by using the North-South displacement component obtained from the azimuth PO information. Distinct sources of deformations interact in Afar. Fault movements and magma chamber deflation have accompanied dyke intrusions but quantifying each contribution to the total deformation has been challenging, also due to loss of coherence in the central part of the rift. Here we combined the phase and amplitude information in order to retrieve the full deformation field of repeated intrusions. This allows us to better constrain the fault movements that occur as the dyke propagates as well as the magma movements from individual magma chambers.

Atmospheric particles retrieval using satellite remote sensing: Applications for sandstorms and volcanic clouds

NASA Astrophysics Data System (ADS)

Gu, Yingxin

This thesis is concerned with atmospheric particles produced by sandstorms and volcanic eruptions. Three studies were conducted in order to examine particle retrieval methodology, and apply these towards an improved understanding of large-scale sandstorms. A thermal infrared remote sensing retrieval method developed by Wen and Rose [1994], which retrieves particle sizes, optical depth, and total masses of silicate particles in the volcanic cloud, was applied to an April 07, 2001 sandstorm over northern China, using MODIS. Results indicate that the area of the dust cloud observed was 1.34 million km2, the mean particle radius of the dust was 1.44 mum, and the mean optical depth at 11 mum was 0.79. The mean burden of dust was approximately 4.8 tons/km2 and the main portion of the dust storm on April 07, 2001 contained 6.5 million tons of dust. The results are supported by both independent remote sensing data (TOMS) and in-situ data for a similar event in 1998, therefore suggesting that the technique is appropriate for quantitative analysis of silicate dust clouds. This is the first quantitative evaluation of annual and seasonal dust loading in 2003 produced by Saharan dust storms by satellite remote sensing analysis. The retrieved mean particle effective radii of 2003 dust events are between 1.7--2.6 mum which is small enough to be inhaled and is hazardous to human health. The retrieved yearly dust mass load is 658--690 Tg, which is ˜45% of the annual global mineral dust production. Winter is the heaviest dust loading season in the year 2003, which is more than 5 times larger than that in the summer season in 2003.The mean optical depths at 11 mum in the winter season (around 0.7) are higher than those in the summer season (around 0.5). The results could help both meteorologists and environmental scientists to evaluate and predict the hazard degree caused by Saharan dust storms. (Abstract shortened by UMI.)

Phase-sensitive imaging of the outer retina using optical coherence tomography and adaptive optics

PubMed Central

Jonnal, Ravi S.; Kocaoglu, Omer P.; Wang, Qiang; Lee, Sangyeol; Miller, Donald T.

2011-01-01

The cone photoreceptor’s outer segment (OS) experiences changes in optical path length, both in response to visible stimuli and as a matter of its daily course of renewal and shedding. These changes are of interest, to quantify function in healthy cells and assess dysfunction in diseased ones. While optical coherence tomography (OCT), combined with adaptive optics (AO), has permitted unprecedented three-dimensional resolution in the living retina, it has not generally been able to measure these OS dynamics, whose scale is smaller than OCT’s axial resolution of a few microns. A possible solution is to take advantage of the phase information encoded in the OCT signal. Phase-sensitive implementations of spectral-domain optical coherence tomography (SD-OCT) have been demonstrated, capable of resolving sample axial displacements much smaller than the imaging wavelength, but these have been limited to ex vivo samples. In this paper we present a novel technique for retrieving phase information from OCT volumes of the outer retina. The key component of our technique is quantification of phase differences within the retina. We provide a quantitative analysis of such phase information and show that–when combined with appropriate methods for filtering and unwrapping–it can improve the sensitivity to OS length change by more than an order of magnitude, down to 45 nm, slightly thicker than a single OS disc. We further show that phase sensitivity drops off with retinal eccentricity, and that the best location for phase imaging is close to the fovea. We apply the technique to the measurement of sub-resolution changes in the OS over matters of hours. Using custom software for registration and tracking, these microscopic changes are monitored in hundreds of cones over time. In two subjects, the OS was found to have average elongation rates of 150 nm/hr, values which agree with our previous findings. PMID:22254172

Optomechanical properties of cancer cells revealed by light-induced deformation and quantitative phase microscopy

NASA Astrophysics Data System (ADS)

Kastl, Lena; Budde, Björn; Isbach, Michael; Rommel, Christina; Kemper, Björn; Schnekenburger, Jürgen

2015-05-01

There is a growing interest in cell biology and clinical diagnostics in label-free, optical techniques as the interaction with the sample is minimized and substances like dyes or fixatives do not affect the investigated cells. Such techniques include digital holographic microscopy (DHM) and the optical stretching by fiber optical two beam traps. DHM enables quantitative phase contrast imaging and thereby the determination of the cellular refractive index, dry mass and the volume, whereas optical cell stretching reveals the deformability of cells. Since optical stretching strongly depends on the optical properties and the shape of the investigated material we combined the usage of fiber optical stretching and DHM for the characterization of pancreatic tumor cells. The risk of tumors is their potential to metastasize, spread through the bloodstream and build distal tumors/metastases. The grade of dedifferentiation in which the cells lose their cell type specific properties is a measure for this metastatic potential. The less differentiated the cells are, the higher is their risk to metastasize. Our results demonstrate that pancreatic tumor cells, which are from the same tumor but vary in their grade of differentiation, show significant differences in their deformability. The retrieved data show that differentiated cells have a higher stiffness than less differentiated cells of the same tumor. Even cells that differ only in the expression of a single tumor suppressor gene which is responsible for cell-cell adhesions can be distinguished by their mechanical properties. Additionally, results from DHM measurements yield that the refractive index shows only few variations, indicating that it does not significantly influence optical cell stretching. The obtained results show a promising new approach for the phenotyping of different cell types, especially in tumor cell characterization and cancer diagnostics.

Phase Reconstruction from FROG Using Genetic Algorithms[Frequency-Resolved Optical Gating

DOE Office of Scientific and Technical Information (OSTI.GOV)

Omenetto, F.G.; Nicholson, J.W.; Funk, D.J.

1999-04-12

The authors describe a new technique for obtaining the phase and electric field from FROG measurements using genetic algorithms. Frequency-Resolved Optical Gating (FROG) has gained prominence as a technique for characterizing ultrashort pulses. FROG consists of a spectrally resolved autocorrelation of the pulse to be measured. Typically a combination of iterative algorithms is used, applying constraints from experimental data, and alternating between the time and frequency domain, in order to retrieve an optical pulse. The authors have developed a new approach to retrieving the intensity and phase from FROG data using a genetic algorithm (GA). A GA is a generalmore » parallel search technique that operates on a population of potential solutions simultaneously. Operators in a genetic algorithm, such as crossover, selection, and mutation are based on ideas taken from evolution.« less

Grounding statistical learning in context: The effects of learning and retrieval contexts on cross-situational word learning.

PubMed

Chen, Chi-Hsin; Yu, Chen

2017-06-01

Natural language environments usually provide structured contexts for learning. This study examined the effects of semantically themed contexts-in both learning and retrieval phases-on statistical word learning. Results from 2 experiments consistently showed that participants had higher performance in semantically themed learning contexts. In contrast, themed retrieval contexts did not affect performance. Our work suggests that word learners are sensitive to statistical regularities not just at the level of individual word-object co-occurrences but also at another level containing a whole network of associations among objects and their properties.

Aircraft Measurements of Aerosol Phase Matrix Elements by the Polarized Imaging Nephelometer (Invited)

NASA Astrophysics Data System (ADS)

Dolgos, G.; Martins, J.; Espinosa, R.; Dubovik, O.; Beyersdorf, A. J.; Ziemba, L. D.; Hair, J. W.

2013-12-01

Aerosols have a significant impact on the radiative balance and water cycle of our planet through influencing atmospheric radiation. Remote sensing of aerosols relies on scattering phase matrix information to retrieve aerosol properties with frequent global coverage, the assumed phase matrices must be validated by measurements. At the Laboratory for Aerosols, Clouds and Optics (LACO) at the University of Maryland, Baltimore County (UMBC) we developed a new technique to directly measure the aerosol phase function (P11), the degree of linear polarization of the scattered light (-P12/P11), and the volume scattering coefficient (SCAT). We designed and built a portable instrument called the Polarized Imaging Nephelometer (PI-Neph), shown in Figure 1 (a). The PI-Neph successfully participated in dozens of flights of the NASA Development and Evaluation of satellite ValidatiOn Tools by Experimenters (DEVOTE) project and the Deep Convective Clouds and Chemistry (DC3) project and the January and February deployment of the Deriving Information on Surface Conditions from Column and Vertically Resolved Observations Relevant to Air Quality (Discover-AQ) mission. The ambient aerosol enters the PI-Neph through an inlet and the sample is illuminated by laser light (wavelength of 532 nm); the scattered light is imaged by a stationary wide field of view camera in the scattering angle range of 2° to 178° (in some cases stray light limited the scattering angle range to 3° to 176°). Data for P11, P12, and SCAT were taken every 12 seconds, example datasets from DEVOTE of P11 times SCAT are shown on Figure 1 (b). The talk will highlight results from the three field deployments and will show microphysical retrievals from the scattering data. The size distribution and the average complex refractive index of the ambient aerosol ensemble can be retrieved from the data by an algorithm similar to that of AERONET, as illustrated in Figure 1 (c). Particle sphericity can potentially be retrieved as well, this will be investigated in the near future. The instrument will be applied to the validation of aerosol retrievals of AERONET and airborne polarimeters. The PI-Neph instrument has recently been upgraded to three wavelengths, and a second instrument was built as well. The LACO group is active in developing an advanced open path version of the Imaging Nephelometer that does not require an inlet but measures undisturbed particles under the aircraft wing. Figure 1. (a) The Polarized Imaging Nephelometer instrument inside the B200 aircraft of NASA Langley. (b) Phase function times volume scattering coefficient data from DEVOTE. (c) Retrievals of particle size distribution based on the data in panel (b).

The Impact of Retrieval Processes, Age, General Achievement Level, and Test Scoring Scheme for Children's Metacognitive Monitoring and Controlling

ERIC Educational Resources Information Center

Krebs, Saskia Susanne; Roebers, Claudia Maria

2012-01-01

This multi-phase study examined the influence of retrieval processes on children's metacognitive processes in relation to and in interaction with achievement level and age. First, N = 150 9/10- and 11/12-year old high and low achievers watched an educational film and predicted their test performance. Children then solved a cloze test regarding the…

Contralateral Cortical Organisation of Information in Visual Short-Term Memory: Evidence from Lateralized Brain Activity during Retrieval

ERIC Educational Resources Information Center

Fortier-Gauthier, Ulysse; Moffat, Nicolas; Dell'Acqua, Robert; McDonald, John J.; Jolicoeur, Pierre

2012-01-01

We studied brain activity during retention and retrieval phases of two visual short-term memory (VSTM) experiments. Experiment 1 used a balanced memory array, with one color stimulus in each hemifield, followed by a retention interval and a central probe, at the fixation point that designated the target stimulus in memory about which to make a…

Label-free evanescent microscopy for membrane nano-tomography in living cells.

PubMed

Bon, Pierre; Barroca, Thomas; Lévèque-Fort, Sandrine; Fort, Emmanuel

2014-11-01

We show that through-the-objective evanescent microscopy (epi-EM) is a powerful technique to image membranes in living cells. Readily implementable on a standard inverted microscope, this technique enables full-field and real-time tracking of membrane processes without labeling and thus signal fading. In addition, we demonstrate that the membrane/interface distance can be retrieved with 10 nm precision using a multilayer Fresnel model. We apply this nano-axial tomography of living cell membranes to retrieve quantitative information on membrane invagination dynamics. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Computational diffraction tomographic microscopy with transport of intensity equation using a light-emitting diode array

NASA Astrophysics Data System (ADS)

Li, Jiaji; Chen, Qian; Zhang, Jialin; Zuo, Chao

2017-10-01

Optical diffraction tomography (ODT) is an effective label-free technique for quantitatively refractive index imaging, which enables long-term monitoring of the internal three-dimensional (3D) structures and molecular composition of biological cells with minimal perturbation. However, existing optical tomographic methods generally rely on interferometric configuration for phase measurement and sophisticated mechanical systems for sample rotation or beam scanning. Thereby, the measurement is suspect to phase error coming from the coherent speckle, environmental vibrations, and mechanical error during data acquisition process. To overcome these limitations, we present a new ODT technique based on non-interferometric phase retrieval and programmable illumination emitting from a light-emitting diode (LED) array. The experimental system is built based on a traditional bright field microscope, with the light source replaced by a programmable LED array, which provides angle-variable quasi-monochromatic illumination with an angular coverage of +/-37 degrees in both x and y directions (corresponding to an illumination numerical aperture of ˜ 0.6). Transport of intensity equation (TIE) is utilized to recover the phase at different illumination angles, and the refractive index distribution is reconstructed based on the ODT framework under first Rytov approximation. The missing-cone problem in ODT is addressed by using the iterative non-negative constraint algorithm, and the misalignment of the LED array is further numerically corrected to improve the accuracy of refractive index quantification. Experiments on polystyrene beads and thick biological specimens show that the proposed approach allows accurate refractive index reconstruction while greatly reduced the system complexity and environmental sensitivity compared to conventional interferometric ODT approaches.

Optical diffraction tomography microscopy with transport of intensity equation using a light-emitting diode array

NASA Astrophysics Data System (ADS)

Li, Jiaji; Chen, Qian; Zhang, Jialin; Zhang, Zhao; Zhang, Yan; Zuo, Chao

2017-08-01

Optical diffraction tomography (ODT) is an effective label-free technique for quantitatively refractive index imaging, which enables long-term monitoring of the internal three-dimensional (3D) structures and molecular composition of biological cells with minimal perturbation. However, existing optical tomographic methods generally rely on interferometric configuration for phase measurement and sophisticated mechanical systems for sample rotation or beam scanning. Thereby, the measurement is suspect to phase error coming from the coherent speckle, environmental vibrations, and mechanical error during data acquisition process. To overcome these limitations, we present a new ODT technique based on non-interferometric phase retrieval and programmable illumination emitting from a light-emitting diode (LED) array. The experimental system is built based on a traditional bright field microscope, with the light source replaced by a programmable LED array, which provides angle-variable quasi-monochromatic illumination with an angular coverage of ±37 degrees in both x and y directions (corresponding to an illumination numerical aperture of ∼0.6). Transport of intensity equation (TIE) is utilized to recover the phase at different illumination angles, and the refractive index distribution is reconstructed based on the ODT framework under first Rytov approximation. The missing-cone problem in ODT is addressed by using the iterative non-negative constraint algorithm, and the misalignment of the LED array is further numerically corrected to improve the accuracy of refractive index quantification. Experiments on polystyrene beads and thick biological specimens show that the proposed approach allows accurate refractive index reconstruction while greatly reduced the system complexity and environmental sensitivity compared to conventional interferometric ODT approaches.

UP2 400 High Activity Oxide Legacy Waste Retrieval Project Scope and Progress-13048

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chabeuf, Jean-Michel; Varet, Thierry

The High Activity Oxide facility (HAO) reprocessed sheared and dissolved 4500 metric tons of light water reactor fuel the fuel of the emerging light water reactor spent fuel between 1976 and 1998. Over the period, approximately 2200 tons of process waste, composed primarily of sheared hulls, was produced and stored in a vast silo in the first place, and in canisters stored in pools in subsequent years. Upon shutdown of the facility, AREVA D and D Division in La Hague launched a thorough investigation and characterization of the silos and pools content, which then served as input data for themore » definition of a legacy waste retrieval and reconditioning program. Basic design was conducted between 2005 and 2007, and was followed by an optimization phase which lead to the definition of a final scenario and budget, 12% under the initial estimates. The scenario planned for the construction of a retrieval and reconditioning cell to be built on top of the storage silo. The retrieved waste would then be rinsed and sorted, so that hulls could subsequently be sent to La Hague high activity compacting facility, while resins and sludge would be cemented within the retrieval cell. Detailed design was conducted successfully from 2008 until 2011, while a thorough research and development program was conducted in order to qualify each stage of the retrieval and reconditioning process, and assist in the elaboration of the final waste package specification. This R and D program was defined and conducted as a response and mitigation of the major project risks identified during the basic design process. Procurement and site preparatory works were then launched in 2011. By the end of 2012, R and D is nearly completed, the retrieval and reconditioning process have been secured, the final waste package specification is being completed, the first equipment for the retrieval cell is being delivered on site, while preparation works are allowing to free up space above and around the silo, to allow for construction which is scheduled to being during the first semester of 2013. The elaboration of the final waste package is still undergoing and expected to be completed by then end of 2013, following some final elements of R and D required to demonstrate the full compatibility of the package with deep geological repository. The HAO legacy waste retrieval project is so far the largest such project entering operational phase on the site of La Hague. It is on schedule, under budget, and in conformity with the delivery requirements set by the French Safety Authority, as well as other stakeholders. This project paves the way for the successful completion of AREVA La Hague other legacy waste retrieval projects, which are currently being drafted or already in active R and D phase. (authors)« less

The application of time series models to cloud field morphology analysis

NASA Technical Reports Server (NTRS)

Chin, Roland T.; Jau, Jack Y. C.; Weinman, James A.

1987-01-01

A modeling method for the quantitative description of remotely sensed cloud field images is presented. A two-dimensional texture modeling scheme based on one-dimensional time series procedures is adopted for this purpose. The time series procedure used is the seasonal autoregressive, moving average (ARMA) process in Box and Jenkins. Cloud field properties such as directionality, clustering and cloud coverage can be retrieved by this method. It has been demonstrated that a cloud field image can be quantitatively defined by a small set of parameters and synthesized surrogates can be reconstructed from these model parameters. This method enables cloud climatology to be studied quantitatively.

Closed loop adaptive optics for microscopy without a wavefront sensor

PubMed Central

Kner, Peter; Winoto, Lukman; Agard, David A.; Sedat, John W.

2013-01-01

A three-dimensional wide-field image of a small fluorescent bead contains more than enough information to accurately calculate the wavefront in the microscope objective back pupil plane using the phase retrieval technique. The phase-retrieved wavefront can then be used to set a deformable mirror to correct the point-spread function (PSF) of the microscope without the use of a wavefront sensor. This technique will be useful for aligning the deformable mirror in a widefield microscope with adaptive optics and could potentially be used to correct aberrations in samples where small fluorescent beads or other point sources are used as reference beacons. Another advantage is the high resolution of the retrieved wavefont as compared with current Shack-Hartmann wavefront sensors. Here we demonstrate effective correction of the PSF in 3 iterations. Starting from a severely aberrated system, we achieve a Strehl ratio of 0.78 and a greater than 10-fold increase in maximum intensity. PMID:24392198

Chemical thermodynamic representations of and

DOE Office of Scientific and Technical Information (OSTI.GOV)

Besmann, T.M.; Lindemer, T.B.

1984-01-01

All available oxygen potential-temperature-composition data for the calcium fluorite-structure phase were retrieved from the literature and utilized in the development of a binary solid solution representation of the phase. The data and phase relations are found to be best described by a solution of (Pu/sub 4/3/O/sub 2/) and (PuO/sub 2/) with a temperature dependent interaction energy. The fluorite-structure is assumed to be represented by a combination of the binaries and , and thus treated as a solution of (Pu/sub 4/3/O/sub 2/), (PuO/sub 2/), (UO/sub 2/), and either (U/sub 2/O/sub 4/./sub 5/) or (U/sub 3/O/sub 7/). The resulting equations wellmore » reproduce the large amount of oxygen potential-temperature-composition data for the mixed oxide system, all of which were also retrieved from the literature. These models are the first that appear to display the appropriate oxygen potential-temperature-composition and phase relation behavior over the entire range of existence for the phases. 39 refs., 10 figs., 3 tabs.« less

Anomalous expansion of the copper-apical-oxygen distance in superconducting cuprate bilayers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou, Hua; Yacoby, Yizhak; Butko, Vladimir Y.

2010-08-27

We have introduced an improved x-ray phase-retrieval method with unprecedented speed of convergence and precision, and used it to determine with sub-Angstrom resolution the complete atomic structure of epitaxial La{sub 2-x}Sr{sub x}CuO{sub 4} ultrathin films. We focus on superconducting heterostructures built from constituent materials that are not superconducting in bulk samples. Single-phase metallic or superconducting films are also studied for comparison. The results show that this phase-retrieval diffraction method enables accurate measurement of structural modifications in near-surface layers, which may be critically important for elucidation of surface-sensitive experiments. Specifically we find that, while the copper-apical-oxygen distance remains approximately constant inmore » single-phase films, it shows a dramatic increase from the metallic-insulating interface of the bilayer towards the surface by as much as 0.45 {angstrom}. The apical-oxygen displacement is known to have a profound effect on the superconducting transition temperature.« less

A single-image method for x-ray refractive index CT.

PubMed

Mittone, A; Gasilov, S; Brun, E; Bravin, A; Coan, P

2015-05-07

X-ray refraction-based computer tomography imaging is a well-established method for nondestructive investigations of various objects. In order to perform the 3D reconstruction of the index of refraction, two or more raw computed tomography phase-contrast images are usually acquired and combined to retrieve the refraction map (i.e. differential phase) signal within the sample. We suggest an approximate method to extract the refraction signal, which uses a single raw phase-contrast image. This method, here applied to analyzer-based phase-contrast imaging, is employed to retrieve the index of refraction map of a biological sample. The achieved accuracy in distinguishing the different tissues is comparable with the non-approximated approach. The suggested procedure can be used for precise refraction computer tomography with the advantage of a reduction of at least a factor of two of both the acquisition time and the dose delivered to the sample with respect to any of the other algorithms in the literature.

Extending MGS-TES Temperature Retrievals in the Martian Atmosphere up to 90 Km: Retrieval Approach and Results

NASA Technical Reports Server (NTRS)

Feofilov, A. G.; Kutepov, A. A.; Rezac, L.; Smith, M. D.

2015-01-01

This paper describes a methodology for performing a temperature retrieval in the Martian atmosphere in the 50-90 km altitude range using spectrally integrated 15 micrometers C02 limb emissions measured by the Thermal Emission Spectrometer (TES), the thermal infrared spectrometer on board the Mars Global Surveyor (MGS). We demonstrate that temperature retrievals from limb observations in the 75-90 km altitude range require accounting for the non-local thermodynamic equilibrium (non-LTE) populations of the C02(v2) vibrational levels. Using the methodology described in the paper, we have retrieved approximately 1200 individual temperature profiles from MGS TES limb observations in the altitude range between 60 and 90 km. 0ur dataset of retrieved temperature profiles is available for download in supplemental materials of this paper. The temperature retrieval uncertainties are mainly caused by radiance noise, and are estimated to be about 2 K at 60 km and below, 4 K at 70 km, 7 K at 80 km, 10 K at 85 km, and 20 K at 90 km. We compare the retrieved profiles to Mars Climate Database temperature profiles and find good qualitative agreement. Quantitatively, our retrieved profiles are in general warmer and demonstrate strong variability with the following values for bias and standard deviations (in brackets) compared to the Martian Year 24 dataset of the Mars Climate Database: 6 (+/-20) K at 60 km, 7.5 (+/-25) K at 65 km, 9 (+/-27) K at 70 km, 9.5 (+/-27) K at 75 km, 10 (+/-28) K at 80 km, 11 (+/-29) K at 85 km, and 11.5 (+/-31) K at 90 km. Possible reasons for the positive temperature bias are discussed. carbon dioxide molecular vibrations

Validation of High-Resolution MAIAC Aerosol Product over South America

NASA Technical Reports Server (NTRS)

Martins, V. S.; Lyapustin, A.; de Carvalho, L. A. S.; Barbosa, C. C. F.; Novo, E. M. L. M.

2017-01-01

Multiangle Implementation of Atmospheric Correction (MAIAC) is a new Moderate Resolution Imaging Spectroradiometer (MODIS) algorithm that combines time series approach and image processing to derive surface reflectance and atmosphere products, such as aerosol optical depth (AOD) and columnar water vapor (CWV). The quality assessment of MAIAC AOD at 1 km resolution is still lacking across South America. In the present study, critical assessment of MAIAC AOD(sub 550) was performed using ground-truth data from 19 Aerosol Robotic Network (AERONET) sites over South America. Additionally, we validated the MAIAC CWV retrievals using the same AERONET sites. In general, MAIAC AOD Terra/Aqua retrievals show high agreement with ground-based measurements, with a correlation coefficient (R) close to unity (R(sub Terra):0.956 and R(sub Aqua):0.949). MAIAC accuracy depends on the surface properties and comparisons revealed high confidence retrievals over cropland, forest, savanna, and grassland covers, where more than 2/3 (approximately 66%) of retrievals are within the expected error (EE = +/-(0.05 + 0.05 × AOD)) and R exceeding 0.86. However, AOD retrievals over bright surfaces show lower correlation than those over vegetated areas. Both MAIAC Terra and Aqua retrievals are similarly comparable to AERONET AOD over the MODIS lifetime (small bias offset approximately 0.006). Additionally, MAIAC CWV presents quantitative information with R approximatley 0.97 and more than 70% of retrievals within error (+/-15%). Nonetheless, the time series validation shows an upward bias trend in CWV Terra retrievals and systematic negative bias for CWV Aqua. These results contribute to a comprehensive evaluation of MAIAC AOD retrievals as a new atmospheric product for future aerosol studies over South America.

Modulation of the electrophysiological correlates of retrieval cue processing by the specificity of task demands.

PubMed

Johnson, Jeffrey D; Rugg, Michael D

2006-02-03

Retrieval orientation refers to the differential processing of retrieval cues according to the type of information sought from memory (e.g., words vs. pictures). In the present study, event-related potentials (ERPs) were employed to investigate whether the neural correlates of differential retrieval orientations are sensitive to the specificity of the retrieval demands of the test task. In separate study-test phases, subjects encoded lists of intermixed words and pictures, and then undertook one of two retrieval tests, in both of which the retrieval cues were exclusively words. In the recognition test, subjects performed 'old/new' discriminations on the test items, and old items corresponded to only one class of studied material (words or pictures). In the exclusion test, old items corresponded to both classes of study material, and subjects were required to respond 'old' only to test items corresponding to a designated class of material. Thus, demands for retrieval specificity were greater in the exclusion test than during recognition. ERPs elicited by correctly classified new items in the two types of test were contrasted according to whether words or pictures were the sought-for material. Material-dependent ERP effects were evident in both tests, but the effects onset earlier and offset later in the exclusion test. The findings suggest that differential processing of retrieval cues, and hence the adoption of differential retrieval orientations, varies according to the specificity of the retrieval goal.

Benefits of Mars ISRU Regolith Water Processing: A Case Study for the NASA Evolvable Mars Campaign

NASA Technical Reports Server (NTRS)

Kleinhenz, Julie; Paz, Aaron; Mueller, Robert

2016-01-01

ISRU of Mars resources was baselined in 2009 Design Reference Architecture (DRA) 5.0, but only for Oxygen production using atmospheric CO2. The Methane (LCH4) needed for ascent propulsion of the Mars Ascent Vehicle (MAV) would need to be brought from Earth. However: Extracting water from the Martian Regolith enables the production of both Oxygen and Methane from Mars resources: Water resources could also be used for other applications including: Life support, radiation shielding, plant growth, etc. Water extraction was not baselined in DRA5.0 due to perceived difficulties and complexity in processing regolith. The NASA Evolvable Mars Campaign (EMC) requested studies to look at the quantitative benefits and trades of using Mars water ISRUPhase 1: Examined architecture scenarios for regolith water retrieval. Completed October 2015. Phase 2: Deep dive of one architecture concept to look at end-to-end system size, mass, power of a LCH4/LO2 ISRU production system

Evaluating Nurses Acceptance of Hospital Information Systems: A Case Study of a Tertiary Care Hospital.

PubMed

Khalifa, Mohamed

2016-01-01

This study aims at evaluating hospital information systems (HIS) acceptance factors among nurses, in order to provide suggestions for successful HIS implementation. The study used mainly quantitative survey methods to collect data directly from nurses through a questionnaire. The availability of computers in the hospital was one of the most influential factors, with a special emphasis on the unavailability of laptop computers and computers on wheels to facilitate immediate data entry and retrieval when nurses are at the point of care. Nurses believed that HIS might frequently slow down the process of care delivery and increase the time spent by patients inside the hospital especially during slow performance and responsiveness phases. Recommendations were classified into three main areas; improving system performance and availability of computers in the hospital, increasing organizational support in the form of providing training and protected time for nurses' to learn and enhancing users' feedback by listening to their complaints and considering their suggestions.

The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells.

PubMed

Lee, Sang Yun; Park, Hyun Joo; Best-Popescu, Catherine; Jang, Seongsoo; Park, Yong Keun

2015-01-01

Here, we report the results of a study on the effects of ethanol exposure on human red blood cells (RBCs) using quantitative phase imaging techniques at the level of individual cells. Three-dimensional refractive index tomograms and dynamic membrane fluctuations of RBCs were measured using common-path diffraction optical tomography, from which morphological (volume, surface area, and sphericity); biochemical (hemoglobin (Hb) concentration and Hb content); and biomechanical (membrane fluctuation) parameters were retrieved at various concentrations of ethanol. RBCs exposed to the ethanol concentration of 0.1 and 0.3% v/v exhibited cell sphericities higher than those of normal cells. However, mean surface area and sphericity of RBCs in a lethal alcoholic condition (0.5% v/v) are not statistically different with those of healthy RBCs. Meanwhile, significant decreases of Hb content and concentration in RBC cytoplasm at the lethal condition were observed. Furthermore, dynamic fluctuation of RBC membranes increased significantly upon ethanol treatments, indicating ethanol-induced membrane fluidization.

Global retrieval of soil moisture and vegetation properties using data-driven methods

NASA Astrophysics Data System (ADS)

Rodriguez-Fernandez, Nemesio; Richaume, Philippe; Kerr, Yann

2017-04-01

Data-driven methods such as neural networks (NNs) are a powerful tool to retrieve soil moisture from multi-wavelength remote sensing observations at global scale. In this presentation we will review a number of recent results regarding the retrieval of soil moisture with the Soil Moisture and Ocean Salinity (SMOS) satellite, either using SMOS brightness temperatures as input data for the retrieval or using SMOS soil moisture retrievals as reference dataset for the training. The presentation will discuss several possibilities for both the input datasets and the datasets to be used as reference for the supervised learning phase. Regarding the input datasets, it will be shown that NNs take advantage of the synergy of SMOS data and data from other sensors such as the Advanced Scatterometer (ASCAT, active microwaves) and MODIS (visible and infra red). NNs have also been successfully used to construct long time series of soil moisture from the Advanced Microwave Scanning Radiometer - Earth Observing System (AMSR-E) and SMOS. A NN with input data from ASMR-E observations and SMOS soil moisture as reference for the training was used to construct a dataset sharing a similar climatology and without a significant bias with respect to SMOS soil moisture. Regarding the reference data to train the data-driven retrievals, we will show different possibilities depending on the application. Using actual in situ measurements is challenging at global scale due to the scarce distribution of sensors. In contrast, in situ measurements have been successfully used to retrieve SM at continental scale in North America, where the density of in situ measurement stations is high. Using global land surface models to train the NN constitute an interesting alternative to implement new remote sensing surface datasets. In addition, these datasets can be used to perform data assimilation into the model used as reference for the training. This approach has recently been tested at the European Centre for Medium-Range Weather Forecasts (ECMWF). Finally, retrievals using radiative transfer models can also be used as a reference SM dataset for the training phase. This approach was used to retrieve soil moisture from ASMR-E, as mentioned above, and also to implement the official European Space Agency (ESA) SMOS soil moisture product in Near-Real-Time. We will finish with a discussion of the retrieval of vegetation parameters from SMOS observations using data-driven methods.

Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy

NASA Astrophysics Data System (ADS)

Min, Junwei; Yao, Baoli; Ketelhut, Steffi; Kemper, Björn

2017-02-01

The modular combination of optical microscopes with digital holographic microscopy (DHM) has been proven to be a powerful tool for quantitative live cell imaging. The introduction of condenser and different microscope objectives (MO) simplifies the usage of the technique and makes it easier to measure different kinds of specimens with different magnifications. However, the high flexibility of illumination and imaging also causes variable phase aberrations that need to be eliminated for high resolution quantitative phase imaging. The existent phase aberrations compensation methods either require add additional elements into the reference arm or need specimen free reference areas or separate reference holograms to build up suitable digital phase masks. These inherent requirements make them unpractical for usage with highly variable illumination and imaging systems and prevent on-line monitoring of living cells. In this paper, we present a simple numerical method for phase aberration compensation based on the analysis of holograms in spatial frequency domain with capabilities for on-line quantitative phase imaging. From a single shot off-axis hologram, the whole phase aberration can be eliminated automatically without numerical fitting or pre-knowledge of the setup. The capabilities and robustness for quantitative phase imaging of living cancer cells are demonstrated.

Determination of precipitation profiles from airborne passive microwave radiometric measurements

NASA Technical Reports Server (NTRS)

Kummerow, Christian; Hakkarinen, Ida M.; Pierce, Harold F.; Weinman, James A.

1991-01-01

This study presents the first quantitative retrievals of vertical profiles of precipitation derived from multispectral passive microwave radiometry. Measurements of microwave brightness temperature (Tb) obtained by a NASA high-altitude research aircraft are related to profiles of rainfall rate through a multichannel piecewise-linear statistical regression procedure. Statistics for Tb are obtained from a set of cloud radiative models representing a wide variety of convective, stratiform, and anvil structures. The retrieval scheme itself determines which cloud model best fits the observed meteorological conditions. Retrieved rainfall rate profiles are converted to equivalent radar reflectivity for comparison with observed reflectivities from a ground-based research radar. Results for two case studies, a stratiform rain situation and an intense convective thunderstorm, show that the radiometrically derived profiles capture the major features of the observed vertical structure of hydrometer density.

Multi-sensor Improved Sea Surface Temperature (MISST) for GODAE

DTIC Science & Technology

2007-09-30

NAVOCEANO has improved on its methodology to add retrieval error information to the US Navy operational data stream. Quantitative estimates of...hycom.rsmas.miami.edu/ “ POSITIV : Prototype Operational System – ISAR – Temperature Instrumentation for the VOS fleet” CIRA/CSU Joint Hurricane Testbed

A high-throughput urinalysis of abused drugs based on a SPE-LC-MS/MS method coupled with an in-house developed post-analysis data treatment system.

PubMed

Cheng, Wing-Chi; Yau, Tsan-Sang; Wong, Ming-Kei; Chan, Lai-Ping; Mok, Vincent King-Kuen

2006-10-16

A rapid urinalysis system based on SPE-LC-MS/MS with an in-house post-analysis data management system has been developed for the simultaneous identification and semi-quantitation of opiates (morphine, codeine), methadone, amphetamines (amphetamine, methylamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA)), 11-benzodiazepines or their metabolites and ketamine. The urine samples are subjected to automated solid phase extraction prior to analysis by LC-MS (Finnigan Surveyor LC connected to a Finnigan LCQ Advantage) fitted with an Alltech Rocket Platinum EPS C-18 column. With a single point calibration at the cut-off concentration for each analyte, simultaneous identification and semi-quantitation for the above mentioned drugs can be achieved in a 10 min run per urine sample. A computer macro-program package was developed to automatically retrieve appropriate data from the analytical data files, compare results with preset values (such as cut-off concentrations, MS matching scores) of each drug being analyzed and generate user-defined Excel reports to indicate all positive and negative results in batch-wise manner for ease of checking. The final analytical results are automatically copied into an Access database for report generation purposes. Through the use of automation in sample preparation, simultaneous identification and semi-quantitation by LC-MS/MS and a tailored made post-analysis data management system, this new urinalysis system significantly improves the quality of results, reduces the post-data treatment time, error due to data transfer and is suitable for high-throughput laboratory in batch-wise operation.

Serum C-reactive protein concentrations in Nova Scotia Duck Tolling Retrievers with immune-mediated rheumatic disease.

PubMed

Bremer, Hanna Dorotea; Hillström, Anna; Kånåhols, Malin; Hagman, Ragnvi; Hansson-Hamlin, Helene

2017-04-17

Nova Scotia Duck Tolling Retrievers (NSDTRs) are a dog breed often affected by immune-mediated rheumatic disease (IMRD), a disorder characterised by chronic stiffness and joint pain. Most, but not all, dogs with IMRD, have antinuclear antibodies (ANA), which are also commonly present in the autoimmune disease systemic lupus erythematosus (SLE). The clinical and diagnostic findings of IMRD indicate that it is an SLE-related disorder. C-reactive protein (CRP), an acute phase protein, is a quantitative marker of inflammation for many diseases and is used for diagnosing and monitoring systemic inflammation in both humans and dogs. However, in human SLE, CRP concentrations are often elevated but correlate poorly with disease activity; they can be low in individual patients with active disease. The aim of the study was to investigate CRP in a group of NSDTRs with the SLE-related disorder IMRD. The hypothesis was that CRP concentrations would be increased in dogs with IMRD compared to healthy dogs, but that the increase would be mild. Serum CRP concentrations were measured in 18 IMRD-affected NSDTRs and 19 healthy control NSDTRs using two different canine-specific CRP assays. Dogs with IMRD and ANA had higher CRP concentrations than the control dogs, but the concentrations were below the clinical decision limit for systemic inflammation for most of the IMRD dogs. These results indicate that CRP concentrations were increased in dogs with IMRD and ANA, but the increase was mild, similar to what has been observed in human SLE.

Ground Validation Assessments of GPM Core Observatory Science Requirements

NASA Astrophysics Data System (ADS)

Petersen, Walt; Huffman, George; Kidd, Chris; Skofronick-Jackson, Gail

2017-04-01

NASA Global Precipitation Measurement (GPM) Mission science requirements define specific measurement error standards for retrieved precipitation parameters such as rain rate, raindrop size distribution, and falling snow detection on instantaneous temporal scales and spatial resolutions ranging from effective instrument fields of view [FOV], to grid scales of 50 km x 50 km. Quantitative evaluation of these requirements intrinsically relies on GPM precipitation retrieval algorithm performance in myriad precipitation regimes (and hence, assumptions related to physics) and on the quality of ground-validation (GV) data being used to assess the satellite products. We will review GPM GV products, their quality, and their application to assessing GPM science requirements, interleaving measurement and precipitation physical considerations applicable to the approaches used. Core GV data products used to assess GPM satellite products include 1) two minute and 30-minute rain gauge bias-adjusted radar rain rate products and precipitation types (rain/snow) adapted/modified from the NOAA/OU multi-radar multi-sensor (MRMS) product over the continental U.S.; 2) Polarimetric radar estimates of rain rate over the ocean collected using the K-Pol radar at Kwajalein Atoll in the Marshall Islands and the Middleton Island WSR-88D radar located in the Gulf of Alaska; and 3) Multi-regime, field campaign and site-specific disdrometer-measured rain/snow size distribution (DSD), phase and fallspeed information used to derive polarimetric radar-based DSD retrievals and snow water equivalent rates (SWER) for comparison to coincident GPM-estimated DSD and precipitation rates/types, respectively. Within the limits of GV-product uncertainty we demonstrate that the GPM Core satellite meets its basic mission science requirements for a variety of precipitation regimes. For the liquid phase, we find that GPM radar-based products are particularly successful in meeting bias and random error requirements associated with retrievals of rain rate and required +/- 0.5 millimeter error bounds for mass-weighted mean drop diameter. Version-04 (V4) GMI GPROF radiometer-based rain rate products exhibit reasonable agreement with GV, but do not completely meet mission science requirements over the continental U.S. for lighter rain rates (e.g., 1 mm/hr) due to excessive random error ( 75%). Importantly, substantial corrections were made to the V4 GPROF algorithm and preliminary analysis of Version 5 (V5) rain products indicates more robust performance relative to GV. For the frozen phase and a modest GPM requirement to "demonstrate detection of snowfall", DPR products do successfully identify snowfall within the sensitivity and beam sampling limits of the DPR instrument ( 12 dBZ lower limit; lowest clutter-free bins). Similarly, the GPROF algorithm successfully "detects" falling snow and delineates it from liquid precipitation. However, the GV approach to computing falling-snow "detection" statistics is intrinsically tied to GPROF Bayesian algorithm-based thresholds of precipitation "detection" and model analysis temperature, and is not sufficiently tied to SWER. Hence we will also discuss ongoing work to establish the lower threshold SWER for "detection" using combined GV radar, gauge and disdrometer-based case studies.

Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC).

PubMed

Phillips, Zachary F; Chen, Michael; Waller, Laura

2017-01-01

We present a new technique for quantitative phase and amplitude microscopy from a single color image with coded illumination. Our system consists of a commercial brightfield microscope with one hardware modification-an inexpensive 3D printed condenser insert. The method, color-multiplexed Differential Phase Contrast (cDPC), is a single-shot variant of Differential Phase Contrast (DPC), which recovers the phase of a sample from images with asymmetric illumination. We employ partially coherent illumination to achieve resolution corresponding to 2× the objective NA. Quantitative phase can then be used to synthesize DIC and phase contrast images or extract shape and density. We demonstrate amplitude and phase recovery at camera-limited frame rates (50 fps) for various in vitro cell samples and c. elegans in a micro-fluidic channel.

Inverse problems with nonnegative and sparse solutions: algorithms and application to the phase retrieval problem

NASA Astrophysics Data System (ADS)

Quy Muoi, Pham; Nho Hào, Dinh; Sahoo, Sujit Kumar; Tang, Dongliang; Cong, Nguyen Huu; Dang, Cuong

2018-05-01

In this paper, we study a gradient-type method and a semismooth Newton method for minimization problems in regularizing inverse problems with nonnegative and sparse solutions. We propose a special penalty functional forcing the minimizers of regularized minimization problems to be nonnegative and sparse, and then we apply the proposed algorithms in a practical the problem. The strong convergence of the gradient-type method and the local superlinear convergence of the semismooth Newton method are proven. Then, we use these algorithms for the phase retrieval problem and illustrate their efficiency in numerical examples, particularly in the practical problem of optical imaging through scattering media where all the noises from experiment are presented.

Filter Function for Wavefront Sensing Over a Field of View

NASA Technical Reports Server (NTRS)

Dean, Bruce H.

2007-01-01

A filter function has been derived as a means of optimally weighting the wavefront estimates obtained in image-based phase retrieval performed at multiple points distributed over the field of view of a telescope or other optical system. When the data obtained in wavefront sensing and, more specifically, image-based phase retrieval, are used for controlling the shape of a deformable mirror or other optic used to correct the wavefront, the control law obtained by use of the filter function gives a more balanced optical performance over the field of view than does a wavefront-control law obtained by use of a wavefront estimate obtained from a single point in the field of view.

Hanford Waste End Effector Phase I Test Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berglin, Eric J.; Hatchell, Brian K.; Mount, Jason C.

This test plan describes the Phase 1 testing program of the Hanford Waste End Effector (HWEE) at the Washington River Protection Solutions’ Cold Test Facility (CTF) using a Pacific Northwest National Laboratory (PNNL)-designed testing setup. This effort fulfills the informational needs for initial assessment of the HWEE to support Hanford single-shell tank A-105 retrieval. This task will install the HWEE on a PNNL-designed robotic gantry system at CTF, install and calibrate instrumentation to measure reaction forces and process parameters, prepare and characterize simulant materials, and implement the test program. The tests will involve retrieval of water, sludge, and hardpan simulantsmore » to determine pumping rate, dilution factors, and screen fouling rate.« less

Investigation of middle ear anatomy and function with combined video otoscopy-phase sensitive OCT

PubMed Central

Park, Jesung; Cheng, Jeffrey T.; Ferguson, Daniel; Maguluri, Gopi; Chang, Ernest W.; Clancy, Caitlin; Lee, Daniel J.; Iftimia, Nicusor

2016-01-01

We report the development of a novel otoscopy probe for assessing middle ear anatomy and function. Video imaging and phase-sensitive optical coherence tomography are combined within the same optical path. A sound stimuli channel is incorporated as well to study middle ear function. Thus, besides visualizing the morphology of the middle ear, the vibration amplitude and frequency of the eardrum and ossicles are retrieved as well. Preliminary testing on cadaveric human temporal bone models has demonstrated the capability of this instrument for retrieving middle ear anatomy with micron scale resolution, as well as the vibration of the tympanic membrane and ossicles with sub-nm resolution. PMID:26977336

Color image encryption using random transforms, phase retrieval, chaotic maps, and diffusion

NASA Astrophysics Data System (ADS)

Annaby, M. H.; Rushdi, M. A.; Nehary, E. A.

2018-04-01

The recent tremendous proliferation of color imaging applications has been accompanied by growing research in data encryption to secure color images against adversary attacks. While recent color image encryption techniques perform reasonably well, they still exhibit vulnerabilities and deficiencies in terms of statistical security measures due to image data redundancy and inherent weaknesses. This paper proposes two encryption algorithms that largely treat these deficiencies and boost the security strength through novel integration of the random fractional Fourier transforms, phase retrieval algorithms, as well as chaotic scrambling and diffusion. We show through detailed experiments and statistical analysis that the proposed enhancements significantly improve security measures and immunity to attacks.

Model Simulation of Ionosphere Electron Density with Dynamic Transportation and Mechanism of Sporadic E Layers in Lower Part of Ionosphere

NASA Astrophysics Data System (ADS)

Lin, Y. C.; Chu, Y. H.

2015-12-01

There are many physical theories responsible for explanation the generation mechanism of sporadic E (Es) plasma irregularities. In middle latitude, it's generally believed that sporadic E layers occur in vertical ion convergent areas driven by horizontal neutral wind shear. The sporadic E layers appear characteristic of abundant metallic ion species (i.e., Fe+, Mg+, Na+), that lifetime are longer than molecular ions by a factor of several orders, have been demonstrated by rocket-borne mass spectrometric measurements. On the basic of the GPS Radio Occultation (RO), using the scintillations of the GPS signal-to-noise ratio and intense fluctuation of excess phase, the global and seasonal sporadic E layers occurrence rates could be retrieved. In our previous study we found there is averaged 10 kilometers shift in height between the COSMIC-retrieved sporadic E layer occurrence rate and the sporadic E occurrence rate modeled from considering the convergence/divergence of Fe+ vertical flux. There are many reasons that maybe result in the altitude differences, e.g., tidal wind with phase shift, electric field driven force, iron species distributions. In this research, the quantitative analyses for electric field drives Es layers translations in vertical direction are presented. The tidal wind driven sporadic E layers have been simulating by modeling several nonmetallic ions (O+(4S), O+(2D), O+(2p), N+, N2+, O2+, NO+) and metallic ions (Fe+, FeO2+, FeN2+, FeO+) with wind shear transportation. The simulation result shows the Fe+ particles accumulate at zonal wind shear convergent regions and form the thin sporadic E layers. With the electric field taking into account, the whole shape of sporadic E layers vertical shift 2~5 km that depending on what magnitude and direction of electric field is added.

Alpha Oscillations during Incidental Encoding Predict Subsequent Memory for New "Foil" Information.

PubMed

Vogelsang, David A; Gruber, Matthias; Bergström, Zara M; Ranganath, Charan; Simons, Jon S

2018-05-01

People can employ adaptive strategies to increase the likelihood that previously encoded information will be successfully retrieved. One such strategy is to constrain retrieval toward relevant information by reimplementing the neurocognitive processes that were engaged during encoding. Using EEG, we examined the temporal dynamics with which constraining retrieval toward semantic versus nonsemantic information affects the processing of new "foil" information encountered during a memory test. Time-frequency analysis of EEG data acquired during an initial study phase revealed that semantic compared with nonsemantic processing was associated with alpha decreases in a left frontal electrode cluster from around 600 msec after stimulus onset. Successful encoding of semantic versus nonsemantic foils during a subsequent memory test was related to decreases in alpha oscillatory activity in the same left frontal electrode cluster, which emerged relatively late in the trial at around 1000-1600 msec after stimulus onset. Across participants, left frontal alpha power elicited by semantic processing during the study phase correlated significantly with left frontal alpha power associated with semantic foil encoding during the memory test. Furthermore, larger left frontal alpha power decreases elicited by semantic foil encoding during the memory test predicted better subsequent semantic foil recognition in an additional surprise foil memory test, although this effect did not reach significance. These findings indicate that constraining retrieval toward semantic information involves reimplementing semantic encoding operations that are mediated by alpha oscillations and that such reimplementation occurs at a late stage of memory retrieval, perhaps reflecting additional monitoring processes.

The Associative Structure of Memory for Multi-Element Events

PubMed Central

2013-01-01

The hippocampus is thought to be an associative memory “convergence zone,” binding together the multimodal elements of an experienced event into a single engram. This predicts a degree of dependency between the retrieval of the different elements comprising an event. We present data from a series of studies designed to address this prediction. Participants vividly imagined a series of person–location–object events, and memory for these events was assessed across multiple trials of cued retrieval. Consistent with the prediction, a significant level of dependency was found between the retrieval of different elements from the same event. Furthermore, the level of dependency was sensitive both to retrieval task, with higher dependency during cued recall than cued recognition, and to subjective confidence. We propose a simple model, in which events are stored as multiple pairwise associations between individual event elements, and dependency is captured by a common factor that varies across events. This factor may relate to between-events modulation of the strength of encoding, or to a process of within-event “pattern completion” at retrieval. The model predicts the quantitative pattern of dependency in the data when changes in the level of guessing with retrieval task and confidence are taken into account. Thus, we find direct behavioral support for the idea that memory for complex multimodal events depends on the pairwise associations of their constituent elements and that retrieval of the various elements corresponding to the same event reflects a common factor that varies from event to event. PMID:23915127

Aerosol Optical Retrieval and Surface Reflectance from Airborne Remote Sensing Data over Land

PubMed Central

Bassani, Cristiana; Cavalli, Rosa Maria; Pignatti, Stefano

2010-01-01

Quantitative analysis of atmospheric optical properties and surface reflectance can be performed by applying radiative transfer theory in the Atmosphere-Earth coupled system, for the atmospheric correction of hyperspectral remote sensing data. This paper describes a new physically-based algorithm to retrieve the aerosol optical thickness at 550nm (τ550) and the surface reflectance (ρ) from airborne acquired data in the atmospheric window of the Visible and Near-Infrared (VNIR) range. The algorithm is realized in two modules. Module A retrieves τ550 with a minimization algorithm, then Module B retrieves the surface reflectance ρ for each pixel of the image. The method was tested on five remote sensing images acquired by an airborne sensor under different geometric conditions to evaluate the reliability of the method. The results, τ550 and ρ, retrieved from each image were validated with field data contemporaneously acquired by a sun-sky radiometer and a spectroradiometer, respectively. Good correlation index, r, and low root mean square deviations, RMSD, were obtained for the τ550 retrieved by Module A (r2 = 0.75, RMSD = 0.08) and the ρ retrieved by Module B (r2 ≤ 0.9, RMSD ≤ 0.003). Overall, the results are encouraging, indicating that the method is reliable for optical atmospheric studies and the atmospheric correction of airborne hyperspectral images. The method does not require additional at-ground measurements about at-ground reflectance of the reference pixel and aerosol optical thickness. PMID:22163558

Determination of phase from the ridge of CWT using generalized Morse wavelet

NASA Astrophysics Data System (ADS)

Kocahan, Ozlem; Tiryaki, Erhan; Coskun, Emre; Ozder, Serhat

2018-03-01

The selection of wavelet is an important step in order to determine the phase from the fringe patterns. In the present work, a new wavelet for phase retrieval from the ridge of continuous wavelet transform (CWT) is presented. The phase distributions have been extracted from the optical fringe pattern by choosing the zero order generalized morse wavelet (GMW) as a mother wavelet. The aim of the study is to reveal the ways in which the two varying parameters of GMW affect the phase calculation. To show the validity of this method, an experimental study has been conducted by using the diffraction phase microscopy (DPM) setup; consequently, the profiles of red blood cells have been retrieved. The results for the CWT ridge technique with GMW have been compared with the results for the Morlet wavelet and the Paul wavelet; the results are almost identical for Paul and zero order GMW because of their degree of freedom. Also, for further discussion, the Fourier transform and the Stockwell transform have been applied comparatively. The outcome of the comparison reveals that GMWs are highly applicable to the research in various areas, predominantly biomedicine.

GNSS seismometer: Seismic phase recognition of real-time high-rate GNSS deformation waves

NASA Astrophysics Data System (ADS)

Nie, Zhaosheng; Zhang, Rui; Liu, Gang; Jia, Zhige; Wang, Dijin; Zhou, Yu; Lin, Mu

2016-12-01

High-rate global navigation satellite systems (GNSS) can potentially be used as seismometers to capture short-period instantaneous dynamic deformation waves from earthquakes. However, the performance and seismic phase recognition of the GNSS seismometer in the real-time mode, which plays an important role in GNSS seismology, are still uncertain. By comparing the results of accuracy and precision of the real-time solution using a shake table test, we found real-time solutions to be consistent with post-processing solutions and independent of sampling rate. In addition, we analyzed the time series of real-time solutions for shake table tests and recent large earthquakes. The results demonstrated that high-rate GNSS have the ability to retrieve most types of seismic waves, including P-, S-, Love, and Rayleigh waves. The main factor limiting its performance in recording seismic phases is the widely used 1-Hz sampling rate. The noise floor also makes recognition of some weak seismic phases difficult. We concluded that the propagation velocities and path of seismic waves, macro characteristics of the high-rate GNSS array, spatial traces of seismic phases, and incorporation of seismographs are all useful in helping to retrieve seismic phases from the high-rate GNSS time series.

Combining coherent hard X-ray tomographies with phase retrieval to generate three-dimensional models of forming bone

NASA Astrophysics Data System (ADS)

Bortel, Emely L.; Langer, Max; Rack, Alexander; Forien, Jean-Baptiste; Duda, Georg N.; Fratzl, Peter; Zaslansky, Paul

2017-11-01

Holotomography, a phase sensitive synchrotron-based μCT modality, is a quantitative 3D imaging method. By exploiting partial spatial X-ray coherence, bones can be imaged volumetrically with high resolution coupled with impressive density sensitivity. This tomographic method reveals the main characteristics of the important tissue compartments in forming bones, including the rapidly-changing soft tissue and the partially or fully mineralized bone regions, while revealing subtle density differences in 3D. Here we show typical results observed within the growing femur bone midshafts of healthy mice that are 1, 3, 7, 10 and 14 days old (postpartum). Our results make use of partially-coherent synchrotron radiation employing inline Fresnel-propagation in multiple tomographic datasets obtained in the imaging beamline ID19 of the ESRF. The exquisite detail creates maps of the juxtaposed soft, partially mineralized and highly mineralized bone revealing the environment in which bone cells create and shape the matrix. This high resolution 3D data is a step towards creating realistic computational models that may be used to study the dynamic processes involved in bone tissue formation and adaptation. Such data will enhance our understanding of the important biomechanical interactions directing maturation and shaping of the bone micro- and macro-geometries.

Imaging properties and its improvements of scanning/imaging x-ray microscope

DOE Office of Scientific and Technical Information (OSTI.GOV)

Takeuchi, Akihisa, E-mail: take@spring8.or.jp; Uesugi, Kentaro; Suzuki, Yoshio

A scanning / imaging X-ray microscope (SIXM) system has been developed at SPring-8. The SIXM consists of a scanning X-ray microscope with a one-dimensional (1D) X-ray focusing device and an imaging (full-field) X-ray microscope with a 1D X-ray objective. The motivation of the SIXM system is to realize a quantitative and highly-sensitive multimodal 3D X-ray tomography by taking advantages of both the scanning X-ray microscope using multi-pixel detector and the imaging X-ray microscope. Data acquisition process of a 2D image is completely different between in the horizontal direction and in the vertical direction; a 1D signal is obtained with themore » linear-scanning while the other dimensional signal is obtained with the imaging optics. Such condition have caused a serious problem on the imaging properties that the imaging quality in the vertical direction has been much worse than that in the horizontal direction. In this paper, two approaches to solve this problem will be presented. One is introducing a Fourier transform method for phase retrieval from one phase derivative image, and the other to develop and employ a 1D diffuser to produce an asymmetrical coherent illumination.« less

Retrieval under stress decreases the long-term expression of a human declarative memory via reconsolidation.

PubMed

Larrosa, Pablo Nicolás Fernández; Ojea, Alejandro; Ojea, Ignacio; Molina, Victor Alejandro; Zorrilla-Zubilete, María Aurelia; Delorenzi, Alejandro

2017-07-01

Acute stress impairs memory retrieval of several types of memories. An increase in glucocorticoids, several minutes after stressful events, is described as essential to the impairing retrieval-effects of stressors. Moreover, memory retrieval under stress can have long-term consequences. Through what process does the reactivated memory under stress, despite the disrupting retrieval effects, modify long-term memories? The reconsolidation hypothesis proposes that a previously consolidated memory reactivated by a reminder enters a vulnerability phase (labilization) during which it is transiently sensitive to modulation, followed by a re-stabilization phase. However, previous studies show that the expression of memories during reminder sessions is not a condition to trigger the reconsolidation process since unexpressed memories can be reactivated and labilized. Here we evaluate whether it is possible to reactivate-labilize a memory under the impairing-effects of a mild stressor. We used a paradigm of human declarative memory whose reminder structure allows us to differentiate between a reactivated-labile memory state and a reactivated but non-labile state. Subjects memorized a list of five cue-syllables associated with their respective response-syllables. Seventy-two hours later, results showed that the retrieval of the paired-associate memory was impaired when tested 20min after a mild stressor (cold pressor stress (CPS)) administration, coincident with cortisol levels increase. Then, we investigated the long-term effects of CPS administration prior to the reminder session. Under conditions where the reminder initiates the reconsolidation process, CPS impaired the long-term memory expression tested 24h later. In contrast, CPS did not show effects when administered before a reminder session that does not trigger reconsolidation. Results showed that memory reactivation-labilization occurs even when retrieval was impaired. Memory reactivation under stress could hinder -via reconsolidation- the probability of the traces to be expressed in the long term. Copyright © 2017 Elsevier Inc. All rights reserved.