Characterizing the heterogeneity of tumor tissues from spatially resolved molecular measures

PubMed Central

Zavodszky, Maria I.

2017-01-01

Background Tumor heterogeneity can manifest itself by sub-populations of cells having distinct phenotypic profiles expressed as diverse molecular, morphological and spatial distributions. This inherent heterogeneity poses challenges in terms of diagnosis, prognosis and efficient treatment. Consequently, tools and techniques are being developed to properly characterize and quantify tumor heterogeneity. Multiplexed immunofluorescence (MxIF) is one such technology that offers molecular insight into both inter-individual and intratumor heterogeneity. It enables the quantification of both the concentration and spatial distribution of 60+ proteins across a tissue section. Upon bioimage processing, protein expression data can be generated for each cell from a tissue field of view. Results The Multi-Omics Heterogeneity Analysis (MOHA) tool was developed to compute tissue heterogeneity metrics from MxIF spatially resolved tissue imaging data. This technique computes the molecular state of each cell in a sample based on a pathway or gene set. Spatial states are then computed based on the spatial arrangements of the cells as distinguished by their respective molecular states. MOHA computes tissue heterogeneity metrics from the distributions of these molecular and spatially defined states. A colorectal cancer cohort of approximately 700 subjects with MxIF data is presented to demonstrate the MOHA methodology. Within this dataset, statistically significant correlations were found between the intratumor AKT pathway state diversity and cancer stage and histological tumor grade. Furthermore, intratumor spatial diversity metrics were found to correlate with cancer recurrence. Conclusions MOHA provides a simple and robust approach to characterize molecular and spatial heterogeneity of tissues. Research projects that generate spatially resolved tissue imaging data can take full advantage of this useful technique. The MOHA algorithm is implemented as a freely available R script (see

Role of density modulation in the spatially resolved dynamics of strongly confined liquids

DOE Office of Scientific and Technical Information (OSTI.GOV)

Saw, Shibu, E-mail: shibu.saw@sydney.edu.au; Dasgupta, Chandan, E-mail: cdgupta@physics.iisc.ernet.in

Confinement by walls usually produces a strong modulation in the density of dense liquids near the walls. Using molecular dynamics simulations, we examine the effects of the density modulation on the spatially resolved dynamics of a liquid confined between two parallel walls, using a resolution of a fraction of the interparticle distance in the liquid. The local dynamics is quantified by the relaxation time associated with the temporal autocorrelation function of the local density. We find that this local relaxation time varies in phase with the density modulation. The amplitude of the spatial modulation of the relaxation time can bemore » quite large, depending on the characteristics of the wall and thermodynamic parameters of the liquid. To disentangle the effects of confinement and density modulation on the spatially resolved dynamics, we compare the dynamics of a confined liquid with that of an unconfined one in which a similar density modulation is induced by an external potential. We find several differences indicating that density modulation alone cannot account for all the features seen in the spatially resolved dynamics of confined liquids. We also examine how the dynamics near a wall depends on the separation between the two walls and show that the features seen in our simulations persist in the limit of large wall separation.« less

Spatially resolved D-T(2) correlation NMR of porous media.

PubMed

Zhang, Yan; Blümich, Bernhard

2014-05-01

Within the past decade, 2D Laplace nuclear magnetic resonance (NMR) has been developed to analyze pore geometry and diffusion of fluids in porous media on the micrometer scale. Many objects like rocks and concrete are heterogeneous on the macroscopic scale, and an integral analysis of microscopic properties provides volume-averaged information. Magnetic resonance imaging (MRI) resolves this spatial average on the contrast scale set by the particular MRI technique. Desirable contrast parameters for studies of fluid transport in porous media derive from the pore-size distribution and the pore connectivity. These microscopic parameters are accessed by 1D and 2D Laplace NMR techniques. It is therefore desirable to combine MRI and 2D Laplace NMR to image functional information on fluid transport in porous media. Because 2D Laplace resolved MRI demands excessive measuring time, this study investigates the possibility to restrict the 2D Laplace analysis to the sum signals from low-resolution pixels, which correspond to pixels of similar amplitude in high-resolution images. In this exploratory study spatially resolved D-T2 correlation maps from glass beads and mortar are analyzed. Regions of similar contrast are first identified in high-resolution images to locate corresponding pixels in low-resolution images generated with D-T2 resolved MRI for subsequent pixel summation to improve the signal-to-noise ratio of contrast-specific D-T2 maps. This method is expected to contribute valuable information on correlated sample heterogeneity from the macroscopic and the microscopic scales in various types of porous materials including building materials and rock. Copyright © 2014 Elsevier Inc. All rights reserved.

Improved Mechanistic Understanding of Natural Gas Methane Emissions from Spatially Resolved Aircraft Measurements

DOE PAGES

Schwietzke, Stefan; Pétron, Gabrielle; Conley, Stephen; ...

2017-06-05

Divergence in recent oil and gas related methane emission estimates between aircraft studies (basin total for a midday window) and emissions inventories (annualized regional and national statistics) indicate the need for better understanding the experimental design, including temporal and spatial alignment and interpretation of results. In our aircraft-based methane emission estimates in a major U.S. shale gas basin resolved from west to east show (i) similar spatial distributions for 2 days, (ii) strong spatial correlations with reported NG production (R 2 = 0.75) and active gas well pad count (R 2 = 0.81), and (iii) 2× higher emissions in themore » western half (normalized by gas production) despite relatively homogeneous dry gas and well characteristics. Operator reported hourly activity data show that midday episodic emissions from manual liquid unloadings (a routine operation in this basin and elsewhere) could explain ~1/3 of the total emissions detected midday by the aircraft and ~2/3 of the west–east difference in emissions. The 22% emission difference between both days further emphasizes that episodic sources can substantially impact midday methane emissions and that aircraft may detect daily peak emissions rather than daily averages that are generally employed in emissions inventories. And while the aircraft approach is valid, quantitative, and independent, this study sheds new light on the interpretation of previous basin scale aircraft studies, and provides an improved mechanistic understanding of oil and gas related methane emissions.« less

Improved Mechanistic Understanding of Natural Gas Methane Emissions from Spatially Resolved Aircraft Measurements.

PubMed

Schwietzke, Stefan; Pétron, Gabrielle; Conley, Stephen; Pickering, Cody; Mielke-Maday, Ingrid; Dlugokencky, Edward J; Tans, Pieter P; Vaughn, Tim; Bell, Clay; Zimmerle, Daniel; Wolter, Sonja; King, Clark W; White, Allen B; Coleman, Timothy; Bianco, Laura; Schnell, Russell C

2017-06-20

Divergence in recent oil and gas related methane emission estimates between aircraft studies (basin total for a midday window) and emissions inventories (annualized regional and national statistics) indicate the need for better understanding the experimental design, including temporal and spatial alignment and interpretation of results. Our aircraft-based methane emission estimates in a major U.S. shale gas basin resolved from west to east show (i) similar spatial distributions for 2 days, (ii) strong spatial correlations with reported NG production (R 2 = 0.75) and active gas well pad count (R 2 = 0.81), and (iii) 2× higher emissions in the western half (normalized by gas production) despite relatively homogeneous dry gas and well characteristics. Operator reported hourly activity data show that midday episodic emissions from manual liquid unloadings (a routine operation in this basin and elsewhere) could explain ∼1/3 of the total emissions detected midday by the aircraft and ∼2/3 of the west-east difference in emissions. The 22% emission difference between both days further emphasizes that episodic sources can substantially impact midday methane emissions and that aircraft may detect daily peak emissions rather than daily averages that are generally employed in emissions inventories. While the aircraft approach is valid, quantitative, and independent, our study sheds new light on the interpretation of previous basin scale aircraft studies, and provides an improved mechanistic understanding of oil and gas related methane emissions.

Improved Mechanistic Understanding of Natural Gas Methane Emissions from Spatially Resolved Aircraft Measurements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schwietzke, Stefan; Pétron, Gabrielle; Conley, Stephen

Divergence in recent oil and gas related methane emission estimates between aircraft studies (basin total for a midday window) and emissions inventories (annualized regional and national statistics) indicate the need for better understanding the experimental design, including temporal and spatial alignment and interpretation of results. In our aircraft-based methane emission estimates in a major U.S. shale gas basin resolved from west to east show (i) similar spatial distributions for 2 days, (ii) strong spatial correlations with reported NG production (R 2 = 0.75) and active gas well pad count (R 2 = 0.81), and (iii) 2× higher emissions in themore » western half (normalized by gas production) despite relatively homogeneous dry gas and well characteristics. Operator reported hourly activity data show that midday episodic emissions from manual liquid unloadings (a routine operation in this basin and elsewhere) could explain ~1/3 of the total emissions detected midday by the aircraft and ~2/3 of the west–east difference in emissions. The 22% emission difference between both days further emphasizes that episodic sources can substantially impact midday methane emissions and that aircraft may detect daily peak emissions rather than daily averages that are generally employed in emissions inventories. And while the aircraft approach is valid, quantitative, and independent, this study sheds new light on the interpretation of previous basin scale aircraft studies, and provides an improved mechanistic understanding of oil and gas related methane emissions.« less

Retinal ganglion cell distribution and spatial resolving power in elasmobranchs.

PubMed

Lisney, Thomas J; Collin, Shaun P

2008-01-01

The total number, distribution and peak density of presumed retinal ganglion cells was assessed in 10 species of elasmobranch (nine species of shark and one species of batoid) using counts of Nissl-stained cells in retinal wholemounts. The species sampled include a number of active, predatory benthopelagic and pelagic sharks that are found in a variety of coastal and oceanic habitats and represent elasmobranch groups for which information of this nature is currently lacking. The topographic distribution of cells was heterogeneous in all species. Two benthic species, the shark Chiloscyllium punctatum and the batoid Taeniura lymma, have a dorsal or dorso-central horizontal streak of increased cell density, whereas the majority of the benthopelagic and pelagic sharks examined exhibit a more concentric pattern of increasing cell density, culminating in a central area centralis of higher cell density located close to the optic nerve head. The exception is the shark Alopias superciliosus, which possesses a ventral horizontal streak. Variation in retinal ganglion cell topography appears to be related to the visual demands of different habitats and lifestyles, as well as the positioning of the eyes in the head. The upper limits of spatial resolving power were calculated for all 10 species, using peak ganglion cell densities and estimates of focal length taken from cryo-sectioned eyes in combination with information from the literature. Spatial resolving power ranged from 2.02 to 10.56 cycles deg(-1), which is in accordance with previous studies. Species with a lower spatial resolving power tend to be benthic and/or coastal species that feed on benthic invertebrates and fishes. Active, benthopelagic and pelagic species from more oceanic habitats which feed on larger, more active prey, possess a higher resolving power. Additionally, ganglion cells in a juvenile of C. punctatum, were retrogradely-labeled from the optic nerve with biotinylated dextran amine (BDA). A comparison

Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis.

PubMed

Wang, Du; Zhang, Zhaowei; Li, Peiwu; Zhang, Qi; Zhang, Wen

2016-07-14

Rapid and quantitative sensing of aflatoxin B1 with high sensitivity and specificity has drawn increased attention of studies investigating soybean sauce. A sensitive and rapid quantitative immunochromatographic sensing method was developed for the detection of aflatoxin B1 based on time-resolved fluorescence. It combines the advantages of time-resolved fluorescent sensing and immunochromatography. The dynamic range of a competitive and portable immunoassay was 0.3-10.0 µg·kg(-1), with a limit of detection (LOD) of 0.1 µg·kg(-1) and recoveries of 87.2%-114.3%, within 10 min. The results showed good correlation (R² > 0.99) between time-resolved fluorescent immunochromatographic strip test and high performance liquid chromatography (HPLC). Soybean sauce samples analyzed using time-resolved fluorescent immunochromatographic strip test revealed that 64.2% of samples contained aflatoxin B1 at levels ranging from 0.31 to 12.5 µg·kg(-1). The strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique in food safety analysis.

Quantitative metrics for assessment of chemical image quality and spatial resolution

DOE PAGES

Kertesz, Vilmos; Cahill, John F.; Van Berkel, Gary J.

2016-02-28

Rationale: Currently objective/quantitative descriptions of the quality and spatial resolution of mass spectrometry derived chemical images are not standardized. Development of these standardized metrics is required to objectively describe chemical imaging capabilities of existing and/or new mass spectrometry imaging technologies. Such metrics would allow unbiased judgment of intra-laboratory advancement and/or inter-laboratory comparison for these technologies if used together with standardized surfaces. Methods: We developed two image metrics, viz., chemical image contrast (ChemIC) based on signal-to-noise related statistical measures on chemical image pixels and corrected resolving power factor (cRPF) constructed from statistical analysis of mass-to-charge chronograms across features of interest inmore » an image. These metrics, quantifying chemical image quality and spatial resolution, respectively, were used to evaluate chemical images of a model photoresist patterned surface collected using a laser ablation/liquid vortex capture mass spectrometry imaging system under different instrument operational parameters. Results: The calculated ChemIC and cRPF metrics determined in an unbiased fashion the relative ranking of chemical image quality obtained with the laser ablation/liquid vortex capture mass spectrometry imaging system. These rankings were used to show that both chemical image contrast and spatial resolution deteriorated with increasing surface scan speed, increased lane spacing and decreasing size of surface features. Conclusions: ChemIC and cRPF, respectively, were developed and successfully applied for the objective description of chemical image quality and spatial resolution of chemical images collected from model surfaces using a laser ablation/liquid vortex capture mass spectrometry imaging system.« less

Quantitative metrics for assessment of chemical image quality and spatial resolution

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kertesz, Vilmos; Cahill, John F.; Van Berkel, Gary J.

Rationale: Currently objective/quantitative descriptions of the quality and spatial resolution of mass spectrometry derived chemical images are not standardized. Development of these standardized metrics is required to objectively describe chemical imaging capabilities of existing and/or new mass spectrometry imaging technologies. Such metrics would allow unbiased judgment of intra-laboratory advancement and/or inter-laboratory comparison for these technologies if used together with standardized surfaces. Methods: We developed two image metrics, viz., chemical image contrast (ChemIC) based on signal-to-noise related statistical measures on chemical image pixels and corrected resolving power factor (cRPF) constructed from statistical analysis of mass-to-charge chronograms across features of interest inmore » an image. These metrics, quantifying chemical image quality and spatial resolution, respectively, were used to evaluate chemical images of a model photoresist patterned surface collected using a laser ablation/liquid vortex capture mass spectrometry imaging system under different instrument operational parameters. Results: The calculated ChemIC and cRPF metrics determined in an unbiased fashion the relative ranking of chemical image quality obtained with the laser ablation/liquid vortex capture mass spectrometry imaging system. These rankings were used to show that both chemical image contrast and spatial resolution deteriorated with increasing surface scan speed, increased lane spacing and decreasing size of surface features. Conclusions: ChemIC and cRPF, respectively, were developed and successfully applied for the objective description of chemical image quality and spatial resolution of chemical images collected from model surfaces using a laser ablation/liquid vortex capture mass spectrometry imaging system.« less

Spatially resolved heat release rate measurements in turbulent premixed flames

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ayoola, B.O.; Kaminski, C.F.; Balachandran, R.

Heat release rate is a fundamental property of great importance for the theoretical and experimental elucidation of unsteady flame behaviors such as combustion noise, combustion instabilities, and pulsed combustion. Investigations of such thermoacoustic interactions require a reliable indicator of heat release rate capable of resolving spatial structures in turbulent flames. Traditionally, heat release rate has been estimated via OH or CH radical chemiluminescence; however, chemiluminescence suffers from being a line-of-sight technique with limited capability for resolving small-scale structures. In this paper, we report spatially resolved two-dimensional measurements of a quantity closely related to heat release rate. The diagnostic technique usesmore » simultaneous OH and CH{sub 2}O planar laser-induced fluorescence (PLIF), and the pixel-by-pixel product of the OH and CH{sub 2}O PLIF signals has previously been shown to correlate well with local heat release rates. Results from this diagnostic technique, which we refer to as heat release rate imaging (HR imaging), are compared with traditional OH chemiluminescence measurements in several flames. Studies were performed in lean premixed ethylene flames stabilized between opposed jets and with a bluff body. Correlations between bulk strain rates and local heat release rates were obtained and the effects of curvature on heat release rate were investigated. The results show that the heat release rate tends to increase with increasing negative curvature for the flames investigated for which Lewis numbers are greater than unity. This correlation becomes more pronounced as the flame gets closer to global extinction.« less

Temporally and Spatially Resolved Plasma Spectroscopy in Pulsed Laser Deposition of Ultra-Thin Boron Nitride Films (Postprint)

DTIC Science & Technology

2015-04-24

AFRL-RX-WP-JA-2016-0196 TEMPORALLY AND SPATIALLY RESOLVED PLASMA SPECTROSCOPY IN PULSED LASER DEPOSITION OF ULTRA-THIN BORON NITRIDE...AND SPATIALLY RESOLVED PLASMA SPECTROSCOPY IN PULSED LASER DEPOSITION OF ULTRA-THIN BORON NITRIDE FILMS (POSTPRINT) 5a. CONTRACT NUMBER FA8650...distributions within a PVD plasma plume ablated from a boron nitride (BN) target by a KrF laser at different pressures of nitrogen gas were investigated

3D Spatially Resolved Models of the Intracellular Dynamics of the Hepatitis C Genome Replication Cycle

PubMed Central

Reiter, Sebastian; Grillo, Alfio; Herrmann, Eva; Wittum, Gabriel

2017-01-01

Mathematical models of virus dynamics have not previously acknowledged spatial resolution at the intracellular level despite substantial arguments that favor the consideration of intracellular spatial dependence. The replication of the hepatitis C virus (HCV) viral RNA (vRNA) occurs within special replication complexes formed from membranes derived from endoplasmatic reticulum (ER). These regions, termed membranous webs, are generated primarily through specific interactions between nonstructural virus-encoded proteins (NSPs) and host cellular factors. The NSPs are responsible for the replication of the vRNA and their movement is restricted to the ER surface. Therefore, in this study we developed fully spatio-temporal resolved models of the vRNA replication cycle of HCV. Our simulations are performed upon realistic reconstructed cell structures—namely the ER surface and the membranous webs—based on data derived from immunostained cells replicating HCV vRNA. We visualized 3D simulations that reproduced dynamics resulting from interplay of the different components of our models (vRNA, NSPs, and a host factor), and we present an evaluation of the concentrations for the components within different regions of the cell. Thus far, our model is restricted to an internal portion of a hepatocyte and is qualitative more than quantitative. For a quantitative adaption to complete cells, various additional parameters will have to be determined through further in vitro cell biology experiments, which can be stimulated by the results described in the present study. PMID:28973992

A spatially resolved pyrometer for measuring the blackbody temperature of a warm dense plasma

DOE PAGES

Coleman, Joshua Eugene

2016-12-30

A pyrometer has been developed to spatially resolve the blackbody temperature of a radiatively cooling warm dense plasma. The pyrometer is composed of a lens coupled fiber array, Czerny-Turner visible spectrometer, and an intensified gated CCD for the detector. The radiatively cooling warm dense plasma is generated by a ~100-ns-long intense relativistic electron bunch with an energy of 19.1 MeV and a current of 0.2 kA interacting with 100-μm-thick low-Z foils. The continuum spectrum is measured over 250 nm with a low groove density grating. These plasmas emit visible light or blackbody radiation on relatively long time scales (~0.1 tomore » 100 μs). Finally, we presented the diagnostic layout, calibration, and proof-of-principle measurement of a radiatively cooling aluminum plasma, which includes a spatially resolved temperature gradient and the ability to temporally resolve it also.« less

A quantitative and spatially resolved analysis of the performance-bottleneck in high efficiency, planar hybrid perovskite solar cells

DOE PAGES

Draguta, Sergiu; Christians, Jeffrey A.; Morozov, Yurii V.; ...

2018-01-01

Hybrid perovskites represent a potential paradigm shift for the creation of low-cost solar cells. Current power conversion efficiencies (PCEs) exceed 22%. However, despite this, record PCEs are still far from their theoretical Shockley–Queisser limit of 31%. To increase these PCE values, there is a pressing need to understand, quantify and microscopically model charge recombination processes in full working devices. Here, we present a complete microscopic account of charge recombination processes in high efficiency (18–19% PCE) hybrid perovskite (mixed cation and methylammonium lead iodide) solar cells. We employ diffraction-limited optical measurements along with relevant kinetic modeling to establish, for the firstmore » time, local photoluminescence quantum yields, trap densities, trapping efficiencies, charge extraction efficiencies, quasi-Fermi-level splitting, and effective PCE estimates. Correlations between these spatially resolved parameters, in turn, allow us to conclude that intrinsic electron traps in the perovskite active layers limit the performance of these state-of-the-art hybrid perovskite solar cells.« less

A quantitative and spatially resolved analysis of the performance-bottleneck in high efficiency, planar hybrid perovskite solar cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Draguta, Sergiu; Christians, Jeffrey A.; Morozov, Yurii V.

Hybrid perovskites represent a potential paradigm shift for the creation of low-cost solar cells. Current power conversion efficiencies (PCEs) exceed 22%. However, despite this, record PCEs are still far from their theoretical Shockley–Queisser limit of 31%. To increase these PCE values, there is a pressing need to understand, quantify and microscopically model charge recombination processes in full working devices. Here, we present a complete microscopic account of charge recombination processes in high efficiency (18–19% PCE) hybrid perovskite (mixed cation and methylammonium lead iodide) solar cells. We employ diffraction-limited optical measurements along with relevant kinetic modeling to establish, for the firstmore » time, local photoluminescence quantum yields, trap densities, trapping efficiencies, charge extraction efficiencies, quasi-Fermi-level splitting, and effective PCE estimates. Correlations between these spatially resolved parameters, in turn, allow us to conclude that intrinsic electron traps in the perovskite active layers limit the performance of these state-of-the-art hybrid perovskite solar cells.« less

Spatially resolved Spectroscopy of Europa’s Large-scale Compositional Units at 3-4 μm with Keck NIRSPEC

NASA Astrophysics Data System (ADS)

Fischer, P. D.; Brown, M. E.; Trumbo, S. K.; Hand, K. P.

2017-01-01

We present spatially resolved spectroscopic observations of Europa’s surface at 3-4 μm obtained with the near-infrared spectrograph and adaptive optics system on the Keck II telescope. These are the highest quality spatially resolved reflectance spectra of Europa’s surface at 3-4 μm. The observations spatially resolve Europa’s large-scale compositional units at a resolution of several hundred kilometers. The spectra show distinct features and geographic variations associated with known compositional units; in particular, large-scale leading hemisphere chaos shows a characteristic longward shift in peak reflectance near 3.7 μm compared to icy regions. These observations complement previous spectra of large-scale chaos, and can aid efforts to identify the endogenous non-ice species.

Improving time-delay cosmography with spatially resolved kinematics

NASA Astrophysics Data System (ADS)

Shajib, Anowar J.; Treu, Tommaso; Agnello, Adriano

2018-01-01

Strongly gravitational lensed quasars can be used to measure the so-called time-delay distance DΔt, and thus the Hubble constant H0 and other cosmological parameters. Stellar kinematics of the deflector galaxy play an essential role in this measurement by: (i) helping break the mass-sheet degeneracy; (ii) determining in principle the angular diameter distance Dd to the deflector and thus further improving the cosmological constraints. In this paper we simulate observations of lensed quasars with integral field spectrographs and show that spatially resolved kinematics of the deflector enables further progress by helping break the mass-anisotropy degeneracy. Furthermore, we use our simulations to obtain realistic error estimates with current/upcoming instruments like OSIRIS on Keck and NIRSPEC on the James Webb Space Telescope for both distances (typically ∼6 per cent on DΔt and ∼10 per cent on Dd). We use the error estimates to compute cosmological forecasts for the sample of nine lenses that currently have well-measured time delays and deep Hubble Space Telescope images and for a sample of 40 lenses that is projected to be available in a few years through follow-up of candidates found in ongoing wide field surveys. We find that H0 can be measured with 2 per cent (1 per cent) precision from nine (40) lenses in a flat Λcold dark matter cosmology. We study several other cosmological models beyond the flat Λcold dark matter model and find that time-delay lenses with spatially resolved kinematics can greatly improve the precision of the cosmological parameters measured by cosmic microwave background data.

Evolution and enabling capabilities of spatially resolved techniques for the characterization of heterogeneously catalyzed reactions

DOE PAGES

Morgan, Kevin; Touitou, Jamal; Choi, Jae -Soon; ...

2016-01-15

The development and optimization of catalysts and catalytic processes requires knowledge of reaction kinetics and mechanisms. In traditional catalyst kinetic characterization, the gas composition is known at the inlet, and the exit flow is measured to determine changes in concentration. As such, the progression of the chemistry within the catalyst is not known. Technological advances in electromagnetic and physical probes have made visualizing the evolution of the chemistry within catalyst samples a reality, as part of a methodology commonly known as spatial resolution. Herein, we discuss and evaluate the development of spatially resolved techniques, including the evolutions and achievements ofmore » this growing area of catalytic research. The impact of such techniques is discussed in terms of the invasiveness of physical probes on catalytic systems, as well as how experimentally obtained spatial profiles can be used in conjunction with kinetic modeling. Moreover, some aims and aspirations for further evolution of spatially resolved techniques are considered.« less

Quantitative tradeoffs between spatial, temporal, and thermometric resolution of nonresonant Raman thermometry for dynamic experiments.

PubMed

McGrane, Shawn D; Moore, David S; Goodwin, Peter M; Dattelbaum, Dana M

2014-01-01

The ratio of Stokes to anti-Stokes nonresonant spontaneous Raman can provide an in situ thermometer that is noncontact, independent of any material specific parameters or calibrations, can be multiplexed spatially with line imaging, and can be time resolved for dynamic measurements. However, spontaneous Raman cross sections are very small, and thermometric measurements are often limited by the amount of laser energy that can be applied without damaging the sample or changing its temperature appreciably. In this paper, we quantitatively detail the tradeoff space between spatial, temporal, and thermometric accuracy measurable with spontaneous Raman. Theoretical estimates are pinned to experimental measurements to form realistic expectations of the resolution tradeoffs appropriate to various experiments. We consider the effects of signal to noise, collection efficiency, laser heating, pulsed laser ablation, and blackbody emission as limiting factors, provide formulae to help choose optimal conditions and provide estimates relevant to planning experiments along with concrete examples for single-shot measurements.

Determination of Spatially Resolved Tablet Density and Hardness Using Near-Infrared Chemical Imaging (NIR-CI).

PubMed

Talwar, Sameer; Roopwani, Rahul; Anderson, Carl A; Buckner, Ira S; Drennen, James K

2017-08-01

Near-infrared chemical imaging (NIR-CI) combines spectroscopy with digital imaging, enabling spatially resolved analysis and characterization of pharmaceutical samples. Hardness and relative density are critical quality attributes (CQA) that affect tablet performance. Intra-sample density or hardness variability can reveal deficiencies in formulation design or the tableting process. This study was designed to develop NIR-CI methods to predict spatially resolved tablet density and hardness. The method was implemented using a two-step procedure. First, NIR-CI was used to develop a relative density/solid fraction (SF) prediction method for pure microcrystalline cellulose (MCC) compacts only. A partial least squares (PLS) model for predicting SF was generated by regressing the spectra of certain representative pixels selected from each image against the compact SF. Pixel selection was accomplished with a threshold based on the Euclidean distance from the median tablet spectrum. Second, micro-indentation was performed on the calibration compacts to obtain hardness values. A univariate model was developed by relating the empirical hardness values to the NIR-CI predicted SF at the micro-indented pixel locations: this model generated spatially resolved hardness predictions for the entire tablet surface.

Spatially and momentum resolved energy electron loss spectra from an ultra-thin PrNiO{sub 3} layer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kinyanjui, M. K., E-mail: michael.kinyanjui@uni-ulm.de; Kaiser, U.; Benner, G.

2015-05-18

We present an experimental approach which allows for the acquisition of spectra from ultra-thin films at high spatial, momentum, and energy resolutions. Spatially and momentum (q) resolved electron energy loss spectra have been obtained from a 12 nm ultra-thin PrNiO{sub 3} layer using a nano-beam electron diffraction based approach which enabled the acquisition of momentum resolved spectra from individual, differently oriented nano-domains and at different positions of the PrNiO{sub 3} thin layer. The spatial and wavelength dependence of the spectral excitations are obtained and characterized after the analysis of the experimental spectra using calculated dielectric and energy loss functions. The presentedmore » approach makes a contribution towards obtaining momentum-resolved spectra from nanostructures, thin film, heterostructures, surfaces, and interfaces.« less

Spatially resolved dielectric constant of confined water and its connection to the non-local nature of bulk water

NASA Astrophysics Data System (ADS)

Schaaf, Christian; Gekle, Stephan

2016-08-01

We use molecular dynamics simulations to compute the spatially resolved static dielectric constant of water in cylindrical and spherical nanopores as occurring, e.g., in protein water pockets or carbon nanotubes. For this, we derive a linear-response formalism which correctly takes into account the dielectric boundary conditions in the considered geometries. We find that in cylindrical confinement, the axial component behaves similar as the local density akin to what is known near planar interfaces. The radial dielectric constant shows some oscillatory features when approaching the surface if their radius is larger than about 2 nm. Most importantly, however, the radial component exhibits pronounced oscillations at the center of the cavity. These surprising features are traced back quantitatively to the non-local dielectric nature of bulk water.

Spatially resolved rest-UV spectroscopy of a prototypical quasar driven superwind at low-z

NASA Astrophysics Data System (ADS)

Johnson, Sean

2017-08-01

Powerful galaxy-wide winds launched by quasars are thought to be a common evolutionary phase of massive galaxies, but observations of this phenomena are scarce. We have conducted a multi-wavelength observational campaign for J1356+1026, a poster-child obscured quasar driving a superwind at z=0.123. J1356+1026 is driving a nuclear molecular outflow and an extended ionized outflow observed as an [OIII] emitting bubble at 10 kpc that is spatially coincident with soft X-ray emission. Quasar-driven winds carry material at a wide range of densities and temperatures making it difficult to measure their energetics and the dominant phases are unknown. Here we propose spatially resolved rest-UV spectroscopy by acquiring circum-nuclear absorption spectra of J1356+1026 and emission spectra of its off-nucleus bubble using COS+G140L. The circum-nuclear spectrum will provide measurements of the outflow velocity through blueshifted absorption while the off-nuclear spectrum of the bubble will measure the ionization state and mechanisms of the outflow through powerful UV diagnostic lines. Together, these spectra will enable a more complete mass, energy and momentum accounting of a spatially resolved quasar driven superwind for the first time. Furthermore, detection of shocked gas through OVI emission will enable us to infer properties of the enigmatic volume-filling, low density component of the wind. To our knowledge, this will be the first spatially resolved rest UV spectroscopy of a quasar wind and the proposed observations will serve as a pilot to guide future HST proposals.

Spatially Resolved Sub-millimeter Continuum Imaging of Neptune with ALMA

NASA Astrophysics Data System (ADS)

Iino, Takahiro; Yamada, Takayoshi

2018-02-01

This paper reports the result of spatially resolved 646 GHz sub-millimeter imaging observation of Neptune obtained by the Atacama Large Millimeter and sub-millimeter Array. The observation was performed in 2012 August as the flux calibration and synthesized beam size were small enough to resolve Neptune’s disk at this time. This analysis aims to constrain the vertical structure of deep and upper-tropospheric South polar hot spot detected previously with mid-IR, millimeter, and centimeter wavelength. The probed atmospheric pressure region estimated by the radiative-transfer method was between 1.0 and 0.6 bar for the nadir and South pole views, respectively. The South polar hot spot was not detected clearly with an uncertainty of 2.1 K. The apparent discontinuity of tropospheric and stratospheric hot spot may be caused by the vertical wind shear of South polar zonal jet.

Improved algorithm for estimating optical properties of food and biological materials using spatially-resolved diffuse reflectance

USDA-ARS?s Scientific Manuscript database

In this research, the inverse algorithm for estimating optical properties of food and biological materials from spatially-resolved diffuse reflectance was optimized in terms of data smoothing, normalization and spatial region of reflectance profile for curve fitting. Monte Carlo simulation was used ...

Reactor for in situ measurements of spatially resolved kinetic data in heterogeneous catalysis

NASA Astrophysics Data System (ADS)

Horn, R.; Korup, O.; Geske, M.; Zavyalova, U.; Oprea, I.; Schlögl, R.

2010-06-01

The present work describes a reactor that allows in situ measurements of spatially resolved kinetic data in heterogeneous catalysis. The reactor design allows measurements up to temperatures of 1300 °C and 45 bar pressure, i.e., conditions of industrial relevance. The reactor involves reactants flowing through a solid catalyst bed containing a sampling capillary with a side sampling orifice through which a small fraction of the reacting fluid (gas or liquid) is transferred into an analytical device (e.g., mass spectrometer, gas chromatograph, high pressure liquid chromatograph) for quantitative analysis. The sampling capillary can be moved with μm resolution in or against flow direction to measure species profiles through the catalyst bed. Rotation of the sampling capillary allows averaging over several scan lines. The position of the sampling orifice is such that the capillary channel through the catalyst bed remains always occupied by the capillary preventing flow disturbance and fluid bypassing. The second function of the sampling capillary is to provide a well which can accommodate temperature probes such as a thermocouple or a pyrometer fiber. If a thermocouple is inserted in the sampling capillary and aligned with the sampling orifice fluid temperature profiles can be measured. A pyrometer fiber can be used to measure the temperature profile of the solid catalyst bed. Spatial profile measurements are demonstrated for methane oxidation on Pt and methane oxidative coupling on Li/MgO, both catalysts supported on reticulated α -Al2O3 foam supports.

Spatially resolved resistance of NiO nanostructures under humid environment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jacobs, Christopher B; Ievlev, Anton; Collins, Liam F

2016-01-01

The spatially resolved electrical response of polycrystalline NiO films composed of 40 nm crystallites was investigated under different relative humidity levels (RH). The topological and electrical properties (surface potential and resistance) were characterized with sub 25nm resolution using Kelvin probe force microscopy (KPFM) and conductive scanning probe microscopy under argon atmosphere at 0%, 50%, and 80% relative humidity. The dimensionality of surface features obtained through autocorrelation analysis of topological maps increased linearly with increased relative humidity, as water was adsorbed onto the film surface. Surface potential decreased from about 280mV to about 100 mV and resistance decreased from about 5more » G to about 3 G , in a nonlinear fashion when relative humidity was increased from 0% to 80%. Spatially resolved surface potential and resistance of the NiO films was found to be heterogeneous throughout the film, with distinct domains that grew in size from about 60 nm to 175 nm at 0% and 80% RH levels, respectively. The heterogeneous character of the topological, surface potential, and resistance properties of the polycrystalline NiO film observed under dry conditions decreased with increased relative humidity, yielding nearly homogeneous surface properties at 80% RH, suggesting that the nanoscale potential and resistance properties converge with the mesoscale properties as water is adsorbed onto the NiO film.« less

Spatially Resolved Observations of the Galactic Center Source IRS 21

NASA Astrophysics Data System (ADS)

Tanner, A.; Ghez, A. M.; Morris, M.; Becklin, E. E.; Cotera, A.; Ressler, M.; Werner, M.; Wizinowich, P.

2002-08-01

We present diffraction-limited 2-25 μm images obtained with the W. M. Keck 10 m telescopes that spatially resolve the cool source IRS 21, one of a small group of enigmatic objects in the central parsec of our Galaxy that have eluded classification. Modeled as a Gaussian, the azimuthally averaged intensity profile of IRS 21 has a half-width at half-maximum (HWHM) size of 650+/-80 AU at 2.2 μm and an average HWHM size of 1600+/-200 AU at mid-infrared wavelengths. These large apparent sizes imply an extended distribution of dust. The mid-infrared color map indicates that IRS 21 is a self-luminous source rather than an externally heated dust clump as originally suggested. The spectral energy distribution has distinct near- and mid-infrared components. A simple radiative transfer code, which simultaneously fits the near- and mid-infrared photometry and intensity profiles, supports a model in which the near-infrared radiation is scattered and extincted light from an embedded central source, while the mid-infrared emission is from thermally reradiating silicate dust. We argue that IRS 21 (and by analogy, the other luminous sources along the Northern Arm) is a massive star experiencing rapid mass loss and plowing through the Northern Arm, thereby generating a bow shock, which is spatially resolved in our observations.

Near-Infrared Spatially Resolved Spectroscopy for Tablet Quality Determination.

PubMed

Igne, Benoît; Talwar, Sameer; Feng, Hanzhou; Drennen, James K; Anderson, Carl A

2015-12-01

Near-infrared (NIR) spectroscopy has become a well-established tool for the characterization of solid oral dosage forms manufacturing processes and finished products. In this work, the utility of a traditional single-point NIR measurement was compared with that of a spatially resolved spectroscopic (SRS) measurement for the determination of tablet assay. Experimental designs were used to create samples that allowed for calibration models to be developed and tested on both instruments. Samples possessing a poor distribution of ingredients (highly heterogeneous) were prepared by under-blending constituents prior to compaction to compare the analytical capabilities of the two NIR methods. The results indicate that SRS can provide spatial information that is usually obtainable only through imaging experiments for the determination of local heterogeneity and detection of abnormal tablets that would not be detected with single-point spectroscopy, thus complementing traditional NIR measurement systems for in-line, and in real-time tablet analysis. © 2015 Wiley Periodicals, Inc. and the American Pharmacists Association.

SPATIALLY RESOLVED SPECTROSCOPY OF EUROPA’S LARGE-SCALE COMPOSITIONAL UNITS AT 3–4 μ m WITH KECK NIRSPEC

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fischer, P. D.; Brown, M. E.; Trumbo, S. K.

2017-01-01

We present spatially resolved spectroscopic observations of Europa’s surface at 3–4 μ m obtained with the near-infrared spectrograph and adaptive optics system on the Keck II telescope. These are the highest quality spatially resolved reflectance spectra of Europa’s surface at 3–4 μ m. The observations spatially resolve Europa’s large-scale compositional units at a resolution of several hundred kilometers. The spectra show distinct features and geographic variations associated with known compositional units; in particular, large-scale leading hemisphere chaos shows a characteristic longward shift in peak reflectance near 3.7 μ m compared to icy regions. These observations complement previous spectra of large-scalemore » chaos, and can aid efforts to identify the endogenous non-ice species.« less

Spatially resolved x-ray fluorescence spectroscopy of beryllium capsule implosions at the NIF

NASA Astrophysics Data System (ADS)

MacDonald, M. J.; Bishel, D. T.; Saunders, A. M.; Scott, H. A.; Kyrala, G.; Kline, J.; MacLaren, S.; Thorn, D. B.; Yi, S. A.; Zylstra, A. B.; Falcone, R. W.; Doeppner, T.

2017-10-01

Beryllium ablators used in indirectly driven inertial confinement fusion implosions are doped with copper to prevent preheat of the cryogenic hydrogen fuel. Here, we present analysis of spatially resolved copper K- α fluorescence spectra from the beryllium ablator layer. It has been shown that K- α fluorescence spectroscopy can be used to measure plasma conditions of partially ionized dopants in high energy density systems. In these experiments, K-shell vacancies in the copper dopant are created by the hotspot emission at stagnation, resulting in K-shell fluorescence at bang time. Spatially resolved copper K- α emission spectra are compared to atomic kinetics and radiation code simulations to infer density and temperature profiles. This work was supported by the US DOE under Grant No. DE-NA0001859, under the auspices of the US DOE by Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344, and by Los Alamos National Laboratory under contract DE-AC52-06NA52396.

Characterization of spatially resolved high resolution x-ray spectrometers for HEDP and light-source experiments

NASA Astrophysics Data System (ADS)

Hill, K. W.; Bitter, M.; Delgado-Aparicio, L.; Efthimion, P.; Pablant, N.; Lu, J.; Beiersdorfer, P.; Chen, H.; Magee, E.

2014-10-01

A high resolution 1D imaging x-ray spectrometer concept comprising a spherically bent crystal and a 2D pixelated detector is being optimized for diagnostics of small sources such as high energy density physics (HEDP) and synchrotron radiation or x-ray free electron laser experiments. This instrument is used on tokamak experiments for measurement of spatial profiles of Doppler ion temperature and plasma flow velocity, as well as electron temperature. Laboratory measurements demonstrate a resolving power, E/ ΔE of 10,000 and spatial resolution better than 10 μm. Good performance is obtained for Bragg angles ranging from 23 to 63 degrees. Initial tests of the instrument on HEDP plasmas are being performed with a goal of developing spatially resolved ion and electron temperature diagnostics. This work was performed under the auspices of the US DOE by PPPL under Contract DE-AC02-09CH11466 and by LLNL under Contract DE-AC52-07NA27344.

Voxel-based measurement sensitivity of spatially resolved near-infrared spectroscopy in layered tissues

NASA Astrophysics Data System (ADS)

Niwayama, Masatsugu

2018-03-01

We quantitatively investigated the measurement sensitivity of spatially resolved spectroscopy (SRS) across six tissue models: cerebral tissue, a small animal brain, the forehead of a fetus, an adult brain, forearm muscle, and thigh muscle. The optical path length in the voxel of the model was analyzed using Monte Carlo simulations. It was found that the measurement sensitivity can be represented as the product of the change in the absorption coefficient and the difference in optical path length in two states with different source-detector distances. The results clarified the sensitivity ratio between the surface layer and the deep layer at each source-detector distance for each model and identified changes in the deep measurement area when one of the detectors was close to the light source. A comparison was made with the results from continuous-wave spectroscopy. The study also identified measurement challenges that arise when the surface layer is inhomogeneous. Findings on the measurement sensitivity of SRS at each voxel and in each layer can support the correct interpretation of measured values when near-infrared oximetry or functional near-infrared spectroscopy is used to investigate different tissue structures.

An approach to estimate spatial distribution of analyte within cells using spectrally-resolved fluorescence microscopy

NASA Astrophysics Data System (ADS)

Sharma, Dharmendar Kumar; Irfanullah, Mir; Basu, Santanu Kumar; Madhu, Sheri; De, Suman; Jadhav, Sameer; Ravikanth, Mangalampalli; Chowdhury, Arindam

2017-03-01

While fluorescence microscopy has become an essential tool amongst chemists and biologists for the detection of various analyte within cellular environments, non-uniform spatial distribution of sensors within cells often restricts extraction of reliable information on relative abundance of analytes in different subcellular regions. As an alternative to existing sensing methodologies such as ratiometric or FRET imaging, where relative proportion of analyte with respect to the sensor can be obtained within cells, we propose a methodology using spectrally-resolved fluorescence microscopy, via which both the relative abundance of sensor as well as their relative proportion with respect to the analyte can be simultaneously extracted for local subcellular regions. This method is exemplified using a BODIPY sensor, capable of detecting mercury ions within cellular environments, characterized by spectral blue-shift and concurrent enhancement of emission intensity. Spectral emission envelopes collected from sub-microscopic regions allowed us to compare the shift in transition energies as well as integrated emission intensities within various intracellular regions. Construction of a 2D scatter plot using spectral shifts and emission intensities, which depend on the relative amount of analyte with respect to sensor and the approximate local amounts of the probe, respectively, enabled qualitative extraction of relative abundance of analyte in various local regions within a single cell as well as amongst different cells. Although the comparisons remain semi-quantitative, this approach involving analysis of multiple spectral parameters opens up an alternative way to extract spatial distribution of analyte in heterogeneous systems. The proposed method would be especially relevant for fluorescent probes that undergo relatively nominal shift in transition energies compared to their emission bandwidths, which often restricts their usage for quantitative ratiometric imaging in

An approach to estimate spatial distribution of analyte within cells using spectrally-resolved fluorescence microscopy.

PubMed

Sharma, Dharmendar Kumar; Irfanullah, Mir; Basu, Santanu Kumar; Madhu, Sheri; De, Suman; Jadhav, Sameer; Ravikanth, Mangalampalli; Chowdhury, Arindam

2017-01-18

While fluorescence microscopy has become an essential tool amongst chemists and biologists for the detection of various analyte within cellular environments, non-uniform spatial distribution of sensors within cells often restricts extraction of reliable information on relative abundance of analytes in different subcellular regions. As an alternative to existing sensing methodologies such as ratiometric or FRET imaging, where relative proportion of analyte with respect to the sensor can be obtained within cells, we propose a methodology using spectrally-resolved fluorescence microscopy, via which both the relative abundance of sensor as well as their relative proportion with respect to the analyte can be simultaneously extracted for local subcellular regions. This method is exemplified using a BODIPY sensor, capable of detecting mercury ions within cellular environments, characterized by spectral blue-shift and concurrent enhancement of emission intensity. Spectral emission envelopes collected from sub-microscopic regions allowed us to compare the shift in transition energies as well as integrated emission intensities within various intracellular regions. Construction of a 2D scatter plot using spectral shifts and emission intensities, which depend on the relative amount of analyte with respect to sensor and the approximate local amounts of the probe, respectively, enabled qualitative extraction of relative abundance of analyte in various local regions within a single cell as well as amongst different cells. Although the comparisons remain semi-quantitative, this approach involving analysis of multiple spectral parameters opens up an alternative way to extract spatial distribution of analyte in heterogeneous systems. The proposed method would be especially relevant for fluorescent probes that undergo relatively nominal shift in transition energies compared to their emission bandwidths, which often restricts their usage for quantitative ratiometric imaging in

Complex EUV imaging reflectometry: spatially resolved 3D composition determination and dopant profiling with a tabletop 13nm source

NASA Astrophysics Data System (ADS)

Porter, Christina L.; Tanksalvala, Michael; Gerrity, Michael; Miley, Galen P.; Esashi, Yuka; Horiguchi, Naoto; Zhang, Xiaoshi; Bevis, Charles S.; Karl, Robert; Johnsen, Peter; Adams, Daniel E.; Kapteyn, Henry C.; Murnane, Margaret M.

2018-03-01

With increasingly 3D devices becoming the norm, there is a growing need in the semiconductor industry and in materials science for high spatial resolution, non-destructive metrology techniques capable of determining depth-dependent composition information on devices. We present a solution to this problem using ptychographic coherent diffractive imaging (CDI) implemented using a commercially available, tabletop 13 nm source. We present the design, simulations, and preliminary results from our new complex EUV imaging reflectometer, which uses coherent 13 nm light produced by tabletop high harmonic generation. This tool is capable of determining spatially-resolved composition vs. depth profiles for samples by recording ptychographic images at multiple incidence angles. By harnessing phase measurements, we can locally and nondestructively determine quantities such as device and thin film layer thicknesses, surface roughness, interface quality, and dopant concentration profiles. Using this advanced imaging reflectometer, we can quantitatively characterize materials-sciencerelevant and industry-relevant nanostructures for a wide variety of applications, spanning from defect and overlay metrology to the development and optimization of nano-enhanced thermoelectric or spintronic devices.

Noncontact blood species identification method based on spatially resolved near-infrared transmission spectroscopy

NASA Astrophysics Data System (ADS)

Zhang, Linna; Sun, Meixiu; Wang, Zhennan; Li, Hongxiao; Li, Yingxin; Li, Gang; Lin, Ling

2017-09-01

The inspection and identification of whole blood are crucially significant for import-export ports and inspection and quarantine departments. In our previous research, we proved Near-Infrared diffuse transmitted spectroscopy method was potential for noninvasively identifying three blood species, including macaque, human and mouse, with samples measured in the cuvettes. However, in open sampling cases, inspectors may be endangered by virulence factors in blood samples. In this paper, we explored the noncontact measurement for classification, with blood samples measured in the vacuum blood vessels. Spatially resolved near-infrared spectroscopy was used to improve the prediction accuracy. Results showed that the prediction accuracy of the model built with nine detection points was more than 90% in identification between all five species, including chicken, goat, macaque, pig and rat, far better than the performance of the model built with single-point spectra. The results fully supported the idea that spatially resolved near-infrared spectroscopy method can improve the prediction ability, and demonstrated the feasibility of this method for noncontact blood species identification in practical applications.

Spatially resolved high resolution x-ray spectroscopy for magnetically confined fusion plasmas (invited)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ince-Cushman, A.; Rice, J. E.; Reinke, M. L.

2008-10-15

The use of high resolution x-ray crystal spectrometers to diagnose fusion plasmas has been limited by the poor spatial localization associated with chord integrated measurements. Taking advantage of a new x-ray imaging spectrometer concept [M. Bitter et al., Rev. Sci. Instrum. 75, 3660 (2004)], and improvements in x-ray detector technology [Ch. Broennimann et al., J. Synchrotron Radiat. 13, 120 (2006)], a spatially resolving high resolution x-ray spectrometer has been built and installed on the Alcator C-Mod tokamak. This instrument utilizes a spherically bent quartz crystal and a set of two dimensional x-ray detectors arranged in the Johann configuration [H. H.more » Johann, Z. Phys. 69, 185 (1931)] to image the entire plasma cross section with a spatial resolution of about 1 cm. The spectrometer was designed to measure line emission from H-like and He-like argon in the wavelength range 3.7 and 4.0 A with a resolving power of approximately 10 000 at frame rates up to 200 Hz. Using spectral tomographic techniques [I. Condrea, Phys. Plasmas 11, 2427 (2004)] the line integrated spectra can be inverted to infer profiles of impurity emissivity, velocity, and temperature. From these quantities it is then possible to calculate impurity density and electron temperature profiles. An overview of the instrument, analysis techniques, and example profiles are presented.« less

Quantitative disentanglement of coherent and incoherent laser-induced surface deformations by time-resolved x-ray reflectivity

NASA Astrophysics Data System (ADS)

Sander, M.; Pudell, J.-E.; Herzog, M.; Bargheer, M.; Bauer, R.; Besse, V.; Temnov, V.; Gaal, P.

2017-12-01

We present time-resolved x-ray reflectivity measurements on laser excited coherent and incoherent surface deformations of thin metallic films. Based on a kinematical diffraction model, we derive the surface amplitude from the diffracted x-ray intensity and resolve transient surface excursions with sub-Å spatial precision and 70 ps temporal resolution. The analysis allows for decomposition of the surface amplitude into multiple coherent acoustic modes and a substantial contribution from incoherent phonons which constitute the sample heating.

A Framework for Widespread Replication of a Highly Spatially Resolved Childhood Lead Exposure Risk Model

PubMed Central

Kim, Dohyeong; Galeano, M. Alicia Overstreet; Hull, Andrew; Miranda, Marie Lynn

2008-01-01

Background Preventive approaches to childhood lead poisoning are critical for addressing this longstanding environmental health concern. Moreover, increasing evidence of cognitive effects of blood lead levels < 10 μg/dL highlights the need for improved exposure prevention interventions. Objectives Geographic information system–based childhood lead exposure risk models, especially if executed at highly resolved spatial scales, can help identify children most at risk of lead exposure, as well as prioritize and direct housing and health-protective intervention programs. However, developing highly resolved spatial data requires labor-and time-intensive geocoding and analytical processes. In this study we evaluated the benefit of increased effort spent geocoding in terms of improved performance of lead exposure risk models. Methods We constructed three childhood lead exposure risk models based on established methods but using different levels of geocoded data from blood lead surveillance, county tax assessors, and the 2000 U.S. Census for 18 counties in North Carolina. We used the results to predict lead exposure risk levels mapped at the individual tax parcel unit. Results The models performed well enough to identify high-risk areas for targeted intervention, even with a relatively low level of effort on geocoding. Conclusions This study demonstrates the feasibility of widespread replication of highly spatially resolved childhood lead exposure risk models. The models guide resource-constrained local health and housing departments and community-based organizations on how best to expend their efforts in preventing and mitigating lead exposure risk in their communities. PMID:19079729

SPATIALLY RESOLVED STAR FORMATION MAIN SEQUENCE OF GALAXIES IN THE CALIFA SURVEY

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cano-Díaz, M.; Sánchez, S. F.; Zibetti, S.

2016-04-20

The “main sequence of galaxies”–defined in terms of the total star formation rate ψ versus the total stellar mass M {sub *}—is a well-studied tight relation that has been observed at several wavelengths and at different redshifts. All earlier studies have derived this relation from integrated properties of galaxies. We recover the same relation from an analysis of spatially resolved properties, with integral field spectroscopic (IFS) observations of 306 galaxies from the CALIFA survey. We consider the SFR surface density in units of log( M {sub ⊙} yr{sup −1} Kpc{sup −2}) and the stellar mass surface density in units ofmore » log( M {sub ⊙} Kpc{sup −2}) in individual spaxels that probe spatial scales of 0.5–1.5 Kpc. This local relation exhibits a high degree of correlation with small scatter ( σ = 0.23 dex), irrespective of the dominant ionization source of the host galaxy or its integrated stellar mass. We highlight (i) the integrated star formation main sequence formed by galaxies whose dominant ionization process is related to star formation, for which we find a slope of 0.81 ± 0.02; (ii) for the spatially resolved relation obtained with the spaxel analysis, we find a slope of 0.72 ± 0.04; and (iii) for the integrated main sequence, we also identified a sequence formed by galaxies that are dominated by an old stellar population, which we have called the retired galaxies sequence.« less

The FAQUIRE Approach: FAst, QUantitative, hIghly Resolved and sEnsitivity Enhanced 1H, 13C Data.

PubMed

Farjon, Jonathan; Milande, Clément; Martineau, Estelle; Akoka, Serge; Giraudeau, Patrick

2018-02-06

The targeted analysis of metabolites in complex mixtures is a challenging issue. NMR is one of the major tools in this field, but there is a strong need for more sensitive, better-resolved, and faster quantitative methods. In this framework, we introduce the concept of FAst, QUantitative, hIghly Resolved and sEnsitivity enhanced (FAQUIRE) NMR to push forward the limits of metabolite NMR analysis. 2D 1 H, 13 C 2D quantitative maps are promising alternatives for enhancing the spectral resolution but are highly time-consuming because of (i) the intrinsic nature of 2D, (ii) the longer recycling times required for quantitative conditions, and (iii) the higher number of scans needed to reduce the level of detection/quantification to access low concentrated metabolites. To reach this aim, speeding up the recently developed QUantItative Perfected and pUre shifted HSQC (QUIPU HSQC) is an interesting attempt to develop the FAQUIRE concept. Thanks to the combination of spectral aliasing, nonuniform sampling, and variable repetition time, the acquisition time of 2D quantitative maps is reduced by a factor 6 to 9, while conserving a high spectral resolution thanks to a pure shift approach. The analytical potential of the new Quick QUIPU HSQC (Q QUIPU HSQC) is evaluated on a model metabolite sample, and its potential is shown on breast-cell extracts embedding metabolites at millimolar to submillimolar concentrations.

Quantitative measurements of magnetic vortices using position resolved diffraction in Lorentz STEM

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zaluzec, N. J.

2002-03-05

A number of electron column techniques have been developed over the last forty years to permit visualization of magnetic fields in specimens. These include: Fresnel imaging, Differential Phase Contrast, Electron Holography and Lorentz STEM. In this work we have extended the LSTEM methodology using Position Resolved Diffraction (PRD) to quantitatively measure the in-plane electromagnetic fields of thin film materials. The experimental work reported herein has been carried out using the ANL AAEM HB603Z 300 kV FEG instrument 5. In this instrument, the electron optical column was operated in a zero field mode, at the specimen, where the objective lens ismore » turned off and the probe forming lens functions were reallocated to the C1, C2, and C3 lenses. Post specimen lenses (P1, P2, P3, P4) were used to magnify the transmitted electrons to a YAG screen, which was then optically transferred to a Hamamatsu ORCA ER CCD array. This CCD was interfaced to an EmiSpec Data Acquisition System and the data was subsequently transferred to an external computer system for detailed quantitative analysis. In Position Resolved Diffraction mode, we digitally step a focused electron probe across the region of interest of the specimen while at the same time recording the complete diffraction pattern at each point in the scan.« less

The quantitative modelling of human spatial habitability

NASA Technical Reports Server (NTRS)

Wise, J. A.

1985-01-01

A model for the quantitative assessment of human spatial habitability is presented in the space station context. The visual aspect assesses how interior spaces appear to the inhabitants. This aspect concerns criteria such as sensed spaciousness and the affective (emotional) connotations of settings' appearances. The kinesthetic aspect evaluates the available space in terms of its suitability to accommodate human movement patterns, as well as the postural and anthrometric changes due to microgravity. Finally, social logic concerns how the volume and geometry of available space either affirms or contravenes established social and organizational expectations for spatial arrangements. Here, the criteria include privacy, status, social power, and proxemics (the uses of space as a medium of social communication).

Spatially Resolved Analysis of Amines Using a Fluorescence Molecular Probe: Molecular Analysis of IDPs

NASA Technical Reports Server (NTRS)

Clemett, S. J.; Messenger, S.; Thomas-Keprta, K. L.; Wentworth, S. J.; Robinson, G. A.; McKay, D. S.

2002-01-01

Some Interplanetary Dust Particles (IDPs) have large isotope anomalies in H and N. To address the nature of the carrier phase, we are developing a procedure to spatially resolve the distribution of organic species on IDP thin sections utilizing fluorescent molecular probes. Additional information is contained in the original extended abstract.

Panchromatic SED modelling of spatially resolved galaxies

NASA Astrophysics Data System (ADS)

Smith, Daniel J. B.; Hayward, Christopher C.

2018-05-01

We test the efficacy of the energy-balance spectral energy distribution (SED) fitting code MAGPHYS for recovering the spatially resolved properties of a simulated isolated disc galaxy, for which it was not designed. We perform 226 950 MAGPHYS SED fits to regions between 0.2 and 25 kpc in size across the galaxy's disc, viewed from three different sight-lines, to probe how well MAGPHYS can recover key galaxy properties based on 21 bands of UV-far-infrared model photometry. MAGPHYS yields statistically acceptable fits to >99 per cent of the pixels within the r-band effective radius and between 59 and 77 percent of pixels within 20 kpc of the nucleus. MAGPHYS is able to recover the distribution of stellar mass, star formation rate (SFR), specific SFR, dust luminosity, dust mass, and V-band attenuation reasonably well, especially when the pixel size is ≳ 1 kpc, whereas non-standard outputs (stellar metallicity and mass-weighted age) are recovered less well. Accurate recovery is more challenging in the smallest sub-regions of the disc (pixel scale ≲ 1 kpc), where the energy balance criterion becomes increasingly incorrect. Estimating integrated galaxy properties by summing the recovered pixel values, the true integrated values of all parameters considered except metallicity and age are well recovered at all spatial resolutions, ranging from 0.2 kpc to integrating across the disc, albeit with some evidence for resolution-dependent biases. These results must be considered when attempting to analyse the structure of real galaxies with actual observational data, for which the `ground truth' is unknown.

Spatially Resolving Ocean Color and Sediment Dispersion in River Plumes, Coastal Systems, and Continental Shelf Waters

NASA Technical Reports Server (NTRS)

Aurin, Dirk Alexander; Mannino, Antonio; Franz, Bryan

2013-01-01

Satellite remote sensing of ocean color in dynamic coastal, inland, and nearshorewaters is impeded by high variability in optical constituents, demands specialized atmospheric correction, and is limited by instrument sensitivity. To accurately detect dispersion of bio-optical properties, remote sensors require ample signal-to-noise ratio (SNR) to sense small variations in ocean color without saturating over bright pixels, an atmospheric correction that can accommodate significantwater-leaving radiance in the near infrared (NIR), and spatial and temporal resolution that coincides with the scales of variability in the environment. Several current and historic space-borne sensors have met these requirements with success in the open ocean, but are not optimized for highly red-reflective and heterogeneous waters such as those found near river outflows or in the presence of sediment resuspension. Here we apply analytical approaches for determining optimal spatial resolution, dominant spatial scales of variability ("patches"), and proportions of patch variability that can be resolved from four river plumes around the world between 2008 and 2011. An offshore region in the Sargasso Sea is analyzed for comparison. A method is presented for processing Moderate Resolution Imaging Spectroradiometer (MODIS) Aqua and Terra imagery including cloud detection, stray lightmasking, faulty detector avoidance, and dynamic aerosol correction using short-wave- and near-infrared wavebands in extremely turbid regions which pose distinct optical and technical challenges. Results showthat a pixel size of approx. 520 mor smaller is generally required to resolve spatial heterogeneity in ocean color and total suspended materials in river plumes. Optimal pixel size increases with distance from shore to approx. 630 m in nearshore regions, approx 750 m on the continental shelf, and approx. 1350 m in the open ocean. Greater than 90% of the optical variability within plume regions is resolvable with

Spatially Resolved Far-Infrared Spectroscopic Analysis of Planetary Nebulae

NASA Astrophysics Data System (ADS)

Rattray, Rebecca; Ueta, Toshiya

2015-01-01

Planetary Nebulae (PNs) are late-life intermediate-mass (1-8 solar mass) stars that have shed their outer layers. A wide variety of morphologies and physical conditions is seen in PNs, but a complete understanding of what causes these various conditions is still needed. Spatially resolved far-infrared spectroscopic analysis has been performed on 11 targets using both PACS and SPIRE instruments on the Herschel Space Observatory as part of the Herschel Planetary Nebula Survey (HerPlaNS). Far-IR lines probe the ionized parts of the nebulae and suffer less extinction than optical lines, so observations in the far-IR are critical to our complete understanding of PNs. Because PNs are extended objects, the spectral mapping capabilities of both PACS and SPIRE allow us to better understand the spatial variations of the objects by tracking line strengths as a function of location within the nebula. The far-IR lines detected in this study can be used as tracers of electron density and electron temperature which are critical parameters in radiative transfer modeling of PNs. Information on atomic, ionic, and molecular lines identified in these 11 targets will be presented.

Synchronous parallel spatially resolved stochastic cluster dynamics

DOE PAGES

Dunn, Aaron; Dingreville, Rémi; Martínez, Enrique; ...

2016-04-23

In this work, a spatially resolved stochastic cluster dynamics (SRSCD) model for radiation damage accumulation in metals is implemented using a synchronous parallel kinetic Monte Carlo algorithm. The parallel algorithm is shown to significantly increase the size of representative volumes achievable in SRSCD simulations of radiation damage accumulation. Additionally, weak scaling performance of the method is tested in two cases: (1) an idealized case of Frenkel pair diffusion and annihilation, and (2) a characteristic example problem including defect cluster formation and growth in α-Fe. For the latter case, weak scaling is tested using both Frenkel pair and displacement cascade damage.more » To improve scaling of simulations with cascade damage, an explicit cascade implantation scheme is developed for cases in which fast-moving defects are created in displacement cascades. For the first time, simulation of radiation damage accumulation in nanopolycrystals can be achieved with a three dimensional rendition of the microstructure, allowing demonstration of the effect of grain size on defect accumulation in Frenkel pair-irradiated α-Fe.« less

Monitoring carbonate dissolution using spatially resolved under-sampled NMR propagators and MRI

NASA Astrophysics Data System (ADS)

Sederman, A. J.; Colbourne, A.; Mantle, M. D.; Gladden, L. F.; Oliveira, R.; Bijeljic, B.; Blunt, M. J.

2017-12-01

The dissolution of a porous rock matrix by an acidic flow causes a change in the pore structure and consequently the pattern of fluid flow and rock permeability. This process is relevant to many areas of practical relevance such as enhanced oil recovery, water contaminant migration and sequestration of supercritical CO2. The most important governing factors for the type of change in the pore space are related by the Péclet (Pe) and Damköhler (Da) dimensionless numbers; these compare the transport properties of the fluid in the porous medium with the reactive properties of the solid matrix and the incident fluid respectively. Variation in Pe and Da can cause very different evolution regimes of the pore space and flow can occur, ranging from a uniform dissolution through different "wormholing" regimes (shown on the left hand side of figure 1) to face dissolution. NMR has a unique capability of measuring both the flow and structural changes during such dissolution whilst the characteristics of flow in the highly heterogeneous matrix that is formed can be predicted by the CTRW modelling approach. Here, NMR measurements of displacement probability distributions, or propagators, have been used to monitor the evolution of fluid flow during a reactive dissolution rock core floods. Developments in the NMR method by undersampling the acquisition data enable spatially resolved measurements of the propagators to be done at sufficient displacement resolution and in a timescale that is short enough to capture the changes in structure and flow. The highly under-sampled (4%) data, which typically reduces the acquisition time from 2 hours to 6 minutes, has been shown to produce equivalent propagator results to the fully sampled experiment. Combining these propagator measurements with quantitative and fast imaging techniques a full time-resolved picture of the dissolution reaction is built up. Experiments have been done for both Ketton and Estaillades carbonate rock cores, which

Spatially resolved photodiode response for simulating precise interferometers.

PubMed

Fernández Barranco, Germán; Tröbs, Michael; Müller, Vitali; Gerberding, Oliver; Seifert, Frank; Heinzel, Gerhard

2016-08-20

Quadrant photodiodes (QPDs) are used in laser interferometry systems to simultaneously detect longitudinal displacement of test masses and angular misalignment between the two interfering beams. The latter is achieved by means of the differential wavefront sensing (DWS) technique, which provides ultra-high precision for measuring angular displacements. We have developed a setup to obtain the spatially resolved response of QPDs that, together with an extension of the simulation software IfoCAD, allows us to use the measured response in simulations and accurately predict the desired longitudinal and DWS phase observables. Three different commercial off-the-shelf QPD candidates for space-based interferometry were characterized. The measured response of one QPD was used in optical simulations. Nonuniformities in the response of the device and crosstalk between segments do not introduce significant variations in the longitudinal and DWS measurands with respect to the standard case when a uniform QPD without crosstalk is used.

ISM DUST GRAINS AND N-BAND SPECTRAL VARIABILITY IN THE SPATIALLY RESOLVED SUBARCSECOND BINARY UY Aur

DOE Office of Scientific and Technical Information (OSTI.GOV)

Skemer, Andrew J.; Close, Laird M.; Hinz, Philip M.

2010-03-10

The 10 {mu}m silicate feature is an essential diagnostic of dust-grain growth and planet formation in young circumstellar disks. The Spitzer Space Telescope has revolutionized the study of this feature, but due to its small (85 cm) aperture, it cannot spatially resolve small/medium-separation binaries ({approx}<3''; {approx}< 420 AU) at the distances of the nearest star-forming regions ({approx}140 pc). Large, 6-10 m ground-based telescopes with mid-infrared instruments can resolve these systems. In this paper, we spatially resolve the 0.''88 binary, UY Aur, with MMTAO/BLINC-MIRAC4 mid-infrared spectroscopy. We then compare our spectra to Spitzer/IRS (unresolved) spectroscopy, and resolved images from IRTF/MIRAC2, Keck/OSCIR,more » and Gemini/Michelle, which were taken over the past decade. We find that UY Aur A has extremely pristine, interstellar medium (ISM)-like grains and that UY Aur B has an unusually shaped silicate feature, which is probably the result of blended emission and absorption from foreground extinction in its disk. We also find evidence for variability in both UY Aur A and UY Aur B by comparing synthetic photometry from our spectra with resolved imaging from previous epochs. The photometric variability of UY Aur A could be an indication that the silicate emission itself is variable, as was recently found in EX Lupi. Otherwise, the thermal continuum is variable, and either the ISM-like dust has never evolved, or it is being replenished, perhaps by UY Aur's circumbinary disk.« less

Spatially resolved NMR spectra for the Swiss cheese model in heavy fermion PuCoGa5 superconductor

NASA Astrophysics Data System (ADS)

Das, Tanmoy; Zhu, Jian-Xin; Balatsky, A. V.; Graf, M. J.

2011-03-01

Spatially resolved NMR experiments, which probe the local electronic excitations, play a vital role for studying the pairing symmetry of unconventional superconductors. Here we calculate the spatial modulation of the NMR spin-lattice relaxation rate (1/T1) for the Swiss cheese model as a function of impurity concentration in PuCoGa5 superconductor. The local suppression of the superconducting order parameter due to impurities is related to the number of holes in the Swiss cheese model. Our results indicate that Friedel-like oscillations,as seen in the local-density of states near an impurity, are also present in the behavior of 1/T1 as one moves away from the impurity site. We demonstrate that the gap nodes, which are filled by disorder, can be probed by NMR through the local information encoded in the spectra. The advantage of spatially resolved NMR compared to STM measurements is that the former probe is not sensitive to surface states. Work is supported by US DOE.

Based on time and spatial-resolved SERS mapping strategies for detection of pesticides.

PubMed

Ma, Bingbing; Li, Pan; Yang, Liangbao; Liu, Jinhuai

2015-08-15

For the sensitive and convenient detection of pesticides, several sensing methods and materials have been widely explored. However, it is still a challenge to obtain sensitive, simple detection techniques for pesticides. Here, the simple and sensitive Time-resolved SERS mapping (T-SERS) and Spatial-resolved SERS mapping (S-SERS) are presented for detection of pesticides by using Au@Ag NPs as SERS substrate. The Time-resolved SERS mapping (T-SERS) is based on state translation nanoparticles from the wet state to the dry state to realize SERS measurements. During the SERS measurement, adhesive force drives the particles closer together and then average interparticle gap becomes smaller. Following, air then begins to intersperse into the liquid network and the particles are held together by adhesive forces at the solid-liquid-air interface. In the late stage of water evaporation, all particles are uniformly distributed. Thus, so called hotspots matrix that can hold hotspots between every two adjacent particles in efficient space with minimal polydispersity of particle size are achieved, accompanying the red-shift of surface plasmon peak and appearance of an optimal SPR resonated sharply with excitation wavelength. Here, we found that the T-SERS method exhibits the detection limits of 1-2 orders of magnitude higher than that of S-SERS. On the other hand, the T-SERS is very simple method with high detection sensitivity, better reproducibility (RSD=10.8%) and is beneficial to construction of a calibration curve in comparison with that of Spatial-resolved SERS mapping (S-SERS). Most importantly, as a result of its remarkable sensitivity, T-SERS mapping strategies have been applied to detection of several pesticides and the detect limit can down to 1nM for paraoxon, 0.5nM for sumithion. In short, T-SERS mapping measurement promises to open a market for SERS practical detection with prominent advantages. Copyright © 2015. Published by Elsevier B.V.

Quantitative analysis of time-resolved microwave conductivity data

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reid, Obadiah G.; Moore, David T.; Li, Zhen

Flash-photolysis time-resolved microwave conductivity (fp-TRMC) is a versatile, highly sensitive technique for studying the complex photoconductivity of solution, solid, and gas-phase samples. The purpose of this paper is to provide a standard reference work for experimentalists interested in using microwave conductivity methods to study functional electronic materials, describing how to conduct and calibrate these experiments in order to obtain quantitative results. The main focus of the paper is on calculating the calibration factor, K, which is used to connect the measured change in microwave power absorption to the conductance of the sample. We describe the standard analytical formulae that havemore » been used in the past, and compare them to numerical simulations. This comparison shows that the most widely used analytical analysis of fp-TRMC data systematically under-estimates the transient conductivity by ~60%. We suggest a more accurate semi-empirical way of calibrating these experiments. However, we emphasize that the full numerical calculation is necessary to quantify both transient and steady-state conductance for arbitrary sample properties and geometry.« less

Quantitative analysis of time-resolved microwave conductivity data

DOE PAGES

Reid, Obadiah G.; Moore, David T.; Li, Zhen; ...

2017-11-10

Flash-photolysis time-resolved microwave conductivity (fp-TRMC) is a versatile, highly sensitive technique for studying the complex photoconductivity of solution, solid, and gas-phase samples. The purpose of this paper is to provide a standard reference work for experimentalists interested in using microwave conductivity methods to study functional electronic materials, describing how to conduct and calibrate these experiments in order to obtain quantitative results. The main focus of the paper is on calculating the calibration factor, K, which is used to connect the measured change in microwave power absorption to the conductance of the sample. We describe the standard analytical formulae that havemore » been used in the past, and compare them to numerical simulations. This comparison shows that the most widely used analytical analysis of fp-TRMC data systematically under-estimates the transient conductivity by ~60%. We suggest a more accurate semi-empirical way of calibrating these experiments. However, we emphasize that the full numerical calculation is necessary to quantify both transient and steady-state conductance for arbitrary sample properties and geometry.« less

Spatially Resolved Mid-IR Spectra from Meteorites; Linking Composition, Crystallographic Orientation and Spectra on the Micro-Scale

NASA Astrophysics Data System (ADS)

Stephen, N. R.

2016-08-01

IR spectroscopy is used to infer composition of extraterrestrial bodies, comparing bulk spectra to databases of separate mineral phases. We extract spatially resolved meteorite-specific spectra from achondrites with respect to zonation and orientation.

Data-driven signal-resolving approaches of infrared spectra to explore the macroscopic and microscopic spatial distribution of organic and inorganic compounds in plant.

PubMed

Chen, Jian-bo; Sun, Su-qin; Zhou, Qun

2015-07-01

The nondestructive and label-free infrared (IR) spectroscopy is a direct tool to characterize the spatial distribution of organic and inorganic compounds in plant. Since plant samples are usually complex mixtures, signal-resolving methods are necessary to find the spectral features of compounds of interest in the signal-overlapped IR spectra. In this research, two approaches using existing data-driven signal-resolving methods are proposed to interpret the IR spectra of plant samples. If the number of spectra is small, "tri-step identification" can enhance the spectral resolution to separate and identify the overlapped bands. First, the envelope bands of the original spectrum are interpreted according to the spectra-structure correlations. Then the spectrum is differentiated to resolve the underlying peaks in each envelope band. Finally, two-dimensional correlation spectroscopy is used to enhance the spectral resolution further. For a large number of spectra, "tri-step decomposition" can resolve the spectra by multivariate methods to obtain the structural and semi-quantitative information about the chemical components. Principal component analysis is used first to explore the existing signal types without any prior knowledge. Then the spectra are decomposed by self-modeling curve resolution methods to estimate the spectra and contents of significant chemical components. At last, targeted methods such as partial least squares target can explore the content profiles of specific components sensitively. As an example, the macroscopic and microscopic distribution of eugenol and calcium oxalate in the bud of clove is studied.

Sensitivity Analysis and Requirements for Temporally and Spatially Resolved Thermometry Using Neutron Resonance Spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fernandez, Juan Carlos; Barnes, Cris William; Mocko, Michael Jeffrey

This report is intended to examine the use of neutron resonance spectroscopy (NRS) to make time- dependent and spatially-resolved temperature measurements of materials in extreme conditions. Specifically, the sensitivities of the temperature estimate on neutron-beam and diagnostic parameters is examined. Based on that examination, requirements are set on a pulsed neutron-source and diagnostics to make a meaningful measurement.

Spatially resolved elemental distributions in articular cartilage

NASA Astrophysics Data System (ADS)

Reinert, T.; Reibetanz, U.; Vogt, J.; Butz, T.; Werner, A.; Gründer, W.

2001-07-01

In this study, the nuclear microprobe technique is employed to analyse the chemistry of joint cartilage in order to correlate internal structures of the collagen network with the elemental distribution. The samples were taken from pig's knee joint. 30 μm thick coronar cross-sections were prepared by means of cryosectioning and freeze-drying. We performed simultaneously particle induced X-ray emission (PIXE), Rutherford backscattering spectrometry (RBS) and elastic recoil detection analysis (ERDA). Thus we obtained spatially resolved distributions of the elements H, C, N, O, P, S, Cl, K and Ca. The main components of the organic matrix are H, C, N and O. It was shown that their relations vary with the cartilage structures. It could be shown that zones with aligned collagen fibrils contain less sulphur and potassium but more chlorine. The higher chlorine concentration is remarkable because newest biochemical studies found that hypochloric acid is involved in cartilage degradation. Furthermore, the calcium distribution is still of great interest. Its correlation to structural changes inside the cartilage is still being discussed. It could be disproved that zones of higher calcium concentration are related to the aligned structures of the collagen network.

Monthly and spatially resolved black carbon emission inventory of India: uncertainty analysis

NASA Astrophysics Data System (ADS)

Paliwal, Umed; Sharma, Mukesh; Burkhart, John F.

2016-10-01

Black carbon (BC) emissions from India for the year 2011 are estimated to be 901.11 ± 151.56 Gg yr-1 based on a new ground-up, GIS-based inventory. The grid-based, spatially resolved emission inventory includes, in addition to conventional sources, emissions from kerosene lamps, forest fires, diesel-powered irrigation pumps and electricity generators at mobile towers. The emissions have been estimated at district level and were spatially distributed onto grids at a resolution of 40 × 40 km2. The uncertainty in emissions has been estimated using a Monte Carlo simulation by considering the variability in activity data and emission factors. Monthly variation of BC emissions has also been estimated to account for the seasonal variability. To the total BC emissions, domestic fuels contributed most significantly (47 %), followed by industry (22 %), transport (17 %), open burning (12 %) and others (2 %). The spatial and seasonal resolution of the inventory will be useful for modeling BC transport in the atmosphere for air quality, global warming and other process-level studies that require greater temporal resolution than traditional inventories.

Spatially resolved spectra of the 'teacup' active galactic nucleus: tracing the history of a dying quasar

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gagne, J. P.; Crenshaw, D. M.; Fischer, T. C.

The Sloan Digital Sky Survey (SDSS) Galaxy Zoo project has revealed a number of spectacular galaxies possessing extended emission-line regions (EELRs), the most famous being Hanny's Voorwerp galaxy. We present another EELR object discovered in the SDSS endeavor: the Teacup active galactic nucleus (AGN). Nicknamed for its EELR, which has a 'handle'-like structure protruding 15 kpc into the northeast quadrant of the galaxy. We analyze the physical conditions of this galaxy with long-slit, ground-based spectroscopy from the Lowell, Lick, and KPNO observatories. With the Lowell 1.8 m Perkin's telescope we took multiple observations at different offset positions, allowing us tomore » recover spatially resolved spectra across the galaxy. Line diagnostics indicate the ionized gas is photoionized primarily by the AGN. Additionally we are able to derive the hydrogen density from the [S II] λ6716/λ6731 ratio. We generated two-component photoionization models for each spatially resolved Lowell spectrum. These models allow us to calculate the AGN bolometric luminosity seen by the gas at different radii from the nuclear center of the Teacup. Our results show a drop in bolometric luminosity by more than two orders of magnitude from the EELR to the nucleus, suggesting that the AGN has decreased in luminosity by this amount in a continuous fashion over 46,000 yr, supporting the case for a dying AGN in this galaxy independent of any IR based evidence. We demonstrate that spatially resolved photoionization modeling could be applied to EELRs to investigate long timescale variability.« less

Spatially resolved chemical analysis of cicada wings using laser-ablation electrospray ionization (LAESI) imaging mass spectrometry (IMS).

PubMed

Román, Jessica K; Walsh, Callee M; Oh, Junho; Dana, Catherine E; Hong, Sungmin; Jo, Kyoo D; Alleyne, Marianne; Miljkovic, Nenad; Cropek, Donald M

2018-03-01

Laser-ablation electrospray ionization (LAESI) imaging mass spectrometry (IMS) is an emerging bioanalytical tool for direct imaging and analysis of biological tissues. Performing ionization in an ambient environment, this technique requires little sample preparation and no additional matrix, and can be performed on natural, uneven surfaces. When combined with optical microscopy, the investigation of biological samples by LAESI allows for spatially resolved compositional analysis. We demonstrate here the applicability of LAESI-IMS for the chemical analysis of thin, desiccated biological samples, specifically Neotibicen pruinosus cicada wings. Positive-ion LAESI-IMS accurate ion-map data was acquired from several wing cells and superimposed onto optical images allowing for compositional comparisons across areas of the wing. Various putative chemical identifications were made indicating the presence of hydrocarbons, lipids/esters, amines/amides, and sulfonated/phosphorylated compounds. With the spatial resolution capability, surprising chemical distribution patterns were observed across the cicada wing, which may assist in correlating trends in surface properties with chemical distribution. Observed ions were either (1) equally dispersed across the wing, (2) more concentrated closer to the body of the insect (proximal end), or (3) more concentrated toward the tip of the wing (distal end). These findings demonstrate LAESI-IMS as a tool for the acquisition of spatially resolved chemical information from fragile, dried insect wings. This LAESI-IMS technique has important implications for the study of functional biomaterials, where understanding the correlation between chemical composition, physical structure, and biological function is critical. Graphical abstract Positive-ion laser-ablation electrospray ionization mass spectrometry coupled with optical imaging provides a powerful tool for the spatially resolved chemical analysis of cicada wings.

Scanning photoelectron microscope for nanoscale three-dimensional spatial-resolved electron spectroscopy for chemical analysis.

PubMed

Horiba, K; Nakamura, Y; Nagamura, N; Toyoda, S; Kumigashira, H; Oshima, M; Amemiya, K; Senba, Y; Ohashi, H

2011-11-01

In order to achieve nondestructive observation of the three-dimensional spatially resolved electronic structure of solids, we have developed a scanning photoelectron microscope system with the capability of depth profiling in electron spectroscopy for chemical analysis (ESCA). We call this system 3D nano-ESCA. For focusing the x-ray, a Fresnel zone plate with a diameter of 200 μm and an outermost zone width of 35 nm is used. In order to obtain the angular dependence of the photoelectron spectra for the depth-profile analysis without rotating the sample, we adopted a modified VG Scienta R3000 analyzer with an acceptance angle of 60° as a high-resolution angle-resolved electron spectrometer. The system has been installed at the University-of-Tokyo Materials Science Outstation beamline, BL07LSU, at SPring-8. From the results of the line-scan profiles of the poly-Si/high-k gate patterns, we achieved a total spatial resolution better than 70 nm. The capability of our system for pinpoint depth-profile analysis and high-resolution chemical state analysis is demonstrated. © 2011 American Institute of Physics

Spatially resolved spectroscopy analysis of the XMM-Newton large program on SN1006

NASA Astrophysics Data System (ADS)

Li, Jiang-Tao; Decourchelle, Anne; Miceli, Marco; Vink, Jacco; Bocchino, Fabrizio

2016-04-01

We perform analysis of the XMM-Newton large program on SN1006 based on our newly developed methods of spatially resolved spectroscopy analysis. We extract spectra from low and high resolution meshes. The former (3596 meshes) is used to roughly decompose the thermal and non-thermal components and characterize the spatial distributions of different parameters, such as temperature, abundances of different elements, ionization age, and electron density of the thermal component, as well as photon index and cutoff frequency of the non-thermal component. On the other hand, the low resolution meshes (583 meshes) focus on the interior region dominated by the thermal emission and have enough counts to well characterize the Si lines. We fit the spectra from the low resolution meshes with different models, in order to decompose the multiple plasma components at different thermal and ionization states and compare their spatial distributions. In this poster, we will present the initial results of this project.

Space-resolved measurements of neutrons and ions emitted by a plasma focus

NASA Astrophysics Data System (ADS)

Jaeger, U.

1980-05-01

Space-resolved measurements of neutrons and of accelerated charged particles emitted by a plasma focus device are presented. The neutron source was measured with one and two dimensional paraffin collimators. The spatial resolution is 5 mn along the axis and the radius, with a time resolution of 10 ns. In order to make quantitative statements about the neutron yield, neutron scattering, absorption, and nuclear reactions were taken into account. Part of the neutron measurement was carried out together with time and space resolved measurements of the electron density to study possible correlations between n sub e and y sub n.

Spatially Resolved Temperature and Water Vapor Concentration Distributions in Supersonic Combustion Facilities by TDLAT

NASA Technical Reports Server (NTRS)

Busa, K. M.; McDaniel J. C.; Diskin, G. S.; DePiro, M. J.; Capriotti, D. P.; Gaffney, R. L.

2012-01-01

Detailed knowledge of the internal structure of high-enthalpy flows can provide valuable insight to the performance of scramjet combustors. Tunable Diode Laser Absorption Spectroscopy (TDLAS) is often employed to measure temperature and species concentration. However, TDLAS is a path-integrated line-of-sight (LOS) measurement, and thus does not produce spatially resolved distributions. Tunable Diode Laser Absorption Tomography (TDLAT) is a non-intrusive measurement technique for determining two-dimensional spatially resolved distributions of temperature and species concentration in high enthalpy flows. TDLAT combines TDLAS with tomographic image reconstruction. More than 2500 separate line-of-sight TDLAS measurements are analyzed in order to produce highly resolved temperature and species concentration distributions. Measurements have been collected at the University of Virginia's Supersonic Combustion Facility (UVaSCF) as well as at the NASA Langley Direct-Connect Supersonic Combustion Test Facility (DCSCTF). Due to the UVaSCF s unique electrical heating and ability for vitiate addition, measurements collected at the UVaSCF are presented as a calibration of the technique. Measurements collected at the DCSCTF required significant modifications to system hardware and software designs due to its larger measurement area and shorter test duration. Tomographic temperature and water vapor concentration distributions are presented from experimentation on the UVaSCF operating at a high temperature non-reacting case for water vitiation level of 12%. Initial LOS measurements from the NASA Langley DCSCTF operating at an equivalence ratio of 0.5 are also presented. Results show the capability of TDLAT to adapt to several experimental setups and test parameters.

Redox-dependent spatially resolved electrochemistry at graphene and graphite step edges.

PubMed

Güell, Aleix G; Cuharuc, Anatolii S; Kim, Yang-Rae; Zhang, Guohui; Tan, Sze-yin; Ebejer, Neil; Unwin, Patrick R

2015-04-28

The electrochemical (EC) behavior of mechanically exfoliated graphene and highly oriented pyrolytic graphite (HOPG) is studied at high spatial resolution in aqueous solutions using Ru(NH3)6(3+/2+) as a redox probe whose standard potential sits close to the intrinsic Fermi level of graphene and graphite. When scanning electrochemical cell microscopy (SECCM) data are coupled with that from complementary techniques (AFM, micro-Raman) applied to the same sample area, different time-dependent EC activity between the basal planes and step edges is revealed. In contrast, other redox couples (ferrocene derivatives) whose potential is further removed from the intrinsic Fermi level of graphene and graphite show uniform and high activity (close to diffusion-control). Macroscopic voltammetric measurements in different environments reveal that the time-dependent behavior after HOPG cleavage, peculiar to Ru(NH3)6(3+/2+), is not associated particularly with any surface contaminants but is reasonably attributed to the spontaneous delamination of the HOPG with time to create partially coupled graphene layers, further supported by conductive AFM measurements. This process has a major impact on the density of states of graphene and graphite edges, particularly at the intrinsic Fermi level to which Ru(NH3)6(3+/2+) is most sensitive. Through the use of an improved voltammetric mode of SECCM, we produce movies of potential-resolved and spatially resolved HOPG activity, revealing how enhanced activity at step edges is a subtle effect for Ru(NH3)6(3+/2+). These latter studies allow us to propose a microscopic model to interpret the EC response of graphene (basal plane and edges) and aged HOPG considering the nontrivial electronic band structure.

Kinematical line broadening and spatially resolved line profiles from AGN.

NASA Astrophysics Data System (ADS)

Schulz, H.; Muecke, A.; Boer, B.; Dresen, M.; Schmidt-Kaler, T.

1995-03-01

We study geometrical effects for emission-line broadening in the optically thin limit by integrating the projected line emissivity along prespecified lines of sight that intersect rotating or expanding disks or cone-like configurations. Analytical expressions are given for the case that emissivity and velocity follow power laws of the radial distance. The results help to interpret spatially resolved spectra and to check the reliability of numerical computations. In the second part we describe a numerical code applicable to any geometrical configuration. Turbulent motions, atmospheric seeing and effects induced by the size of the observing aperture are simulated with appropriate convolution procedures. An application to narrow-line Hα profiles from the central region of the Seyfert galaxy NGC 7469 is presented. The shapes and asymmetries as well as the relative strengths of the Hα lines from different spatial positions can be explained by emission from a nuclear rotating disk of ionized gas, for which the distribution of Hα line emissivity and the rotation curve are derived. Appreciable turbulent line broadening with a Gaussian σ of ~40% of the rotational velocity has to be included to obtain a satisfactory fit.

ISM Dust Grains and N-band Spectral Variability in the Spatially Resolved Subarcsecond Binary UY Aur

NASA Astrophysics Data System (ADS)

Skemer, Andrew J.; Close, Laird M.; Hinz, Philip M.; Hoffmann, William F.; Greene, Thomas P.; Males, Jared R.; Beck, Tracy L.

2010-03-01

The 10 μm silicate feature is an essential diagnostic of dust-grain growth and planet formation in young circumstellar disks. The Spitzer Space Telescope has revolutionized the study of this feature, but due to its small (85 cm) aperture, it cannot spatially resolve small/medium-separation binaries (lsim3''; <~ 420 AU) at the distances of the nearest star-forming regions (~140 pc). Large, 6-10 m ground-based telescopes with mid-infrared instruments can resolve these systems. In this paper, we spatially resolve the 0farcs88 binary, UY Aur, with MMTAO/BLINC-MIRAC4 mid-infrared spectroscopy. We then compare our spectra to Spitzer/IRS (unresolved) spectroscopy, and resolved images from IRTF/MIRAC2, Keck/OSCIR, and Gemini/Michelle, which were taken over the past decade. We find that UY Aur A has extremely pristine, interstellar medium (ISM)-like grains and that UY Aur B has an unusually shaped silicate feature, which is probably the result of blended emission and absorption from foreground extinction in its disk. We also find evidence for variability in both UY Aur A and UY Aur B by comparing synthetic photometry from our spectra with resolved imaging from previous epochs. The photometric variability of UY Aur A could be an indication that the silicate emission itself is variable, as was recently found in EX Lupi. Otherwise, the thermal continuum is variable, and either the ISM-like dust has never evolved, or it is being replenished, perhaps by UY Aur's circumbinary disk. The observations reported here were partially obtained at the Infrared Telescope Facility, which is operated by the University of Hawaii under Cooperative Agreement no. NCC 5-538 with the National Aeronautics and Space Administration, Science Mission Directorate, Planetary Astronomy Program.

Wavevector multiplexed atomic quantum memory via spatially-resolved single-photon detection.

PubMed

Parniak, Michał; Dąbrowski, Michał; Mazelanik, Mateusz; Leszczyński, Adam; Lipka, Michał; Wasilewski, Wojciech

2017-12-15

Parallelized quantum information processing requires tailored quantum memories to simultaneously handle multiple photons. The spatial degree of freedom is a promising candidate to facilitate such photonic multiplexing. Using a single-photon resolving camera, we demonstrate a wavevector multiplexed quantum memory based on a cold atomic ensemble. Observation of nonclassical correlations between Raman scattered photons is confirmed by an average value of the second-order correlation function [Formula: see text] in 665 separated modes simultaneously. The proposed protocol utilizing the multimode memory along with the camera will facilitate generation of multi-photon states, which are a necessity in quantum-enhanced sensing technologies and as an input to photonic quantum circuits.

Quantitative analysis of spatial variability of geotechnical parameters

NASA Astrophysics Data System (ADS)

Fang, Xing

2018-04-01

Geotechnical parameters are the basic parameters of geotechnical engineering design, while the geotechnical parameters have strong regional characteristics. At the same time, the spatial variability of geotechnical parameters has been recognized. It is gradually introduced into the reliability analysis of geotechnical engineering. Based on the statistical theory of geostatistical spatial information, the spatial variability of geotechnical parameters is quantitatively analyzed. At the same time, the evaluation of geotechnical parameters and the correlation coefficient between geotechnical parameters are calculated. A residential district of Tianjin Survey Institute was selected as the research object. There are 68 boreholes in this area and 9 layers of mechanical stratification. The parameters are water content, natural gravity, void ratio, liquid limit, plasticity index, liquidity index, compressibility coefficient, compressive modulus, internal friction angle, cohesion and SP index. According to the principle of statistical correlation, the correlation coefficient of geotechnical parameters is calculated. According to the correlation coefficient, the law of geotechnical parameters is obtained.

Spatially Resolved Imaging and Spectroscopy of Candidate Dual Active Galactic Nuclei

NASA Astrophysics Data System (ADS)

McGurk, R. C.; Max, C. E.; Medling, A. M.; Shields, G. A.; Comerford, J. M.

2015-09-01

When galaxies merge, both central supermassive black holes are immersed in a dense and chaotic environment. If there is sufficient gas in the nuclear regions, one expects to see close pairs of active galactic nuclei (AGNs), or dual AGNs, in a fraction of galaxy mergers. However, finding them remains a challenge. The presence of double-peaked [O iii] emission lines has been proposed as a technique to select dual AGNs efficiently. We studied a sample of double-peaked narrow [O iii] emitting AGNs from Sloan Digital Sky Survey (SDSS) DR7. By obtaining new and archival high spatial resolution images taken with the Keck II Laser Guide Star Adaptive Optics system and the near-infrared camera NIRC2, we show that 30% of 140 double-peaked [O iii] emission line SDSS AGNs have two spatial components within a 3″ radius. However, spatially resolved spectroscopy or X-ray observations are needed to confirm these galaxy pairs as systems containing two AGNs. We followed up three spatially double candidate dual AGNs with integral field spectroscopy from Keck OSIRIS and 10 candidates with long-slit spectroscopy from the Shane Kast Double Spectrograph at Lick Observatory. We find that the double-peaked emission lines in our sample of 12 candidates are caused by: one dual AGN (SDSS J114642.47+511029.6), one confirmed outflow and four likely outflows, two pairs of star-forming galaxies, one candidate indeterminate due to sky line interference, and three AGNs with spatially coincident double [O iii] peaks, likely due to unresolved complex narrow line kinematics, outflows, binary AGN, or small-scale jets.

Spatially resolved proteome mapping of laser capture microdissected tissue with automated sample transfer to nanodroplets.

PubMed

Zhu, Ying; Dou, Maowei; Piehowski, Paul D; Liang, Yiran; Wang, Fangjun; Chu, Rosalie K; Chrisler, Will; Smith, Jordan N; Schwarz, Kaitlynn C; Shen, Yufeng; Shukla, Anil K; Moore, Ronald J; Smith, Richard D; Qian, Wei-Jun; Kelly, Ryan T

2018-06-24

Current mass spectrometry (MS)-based proteomics approaches are ineffective for mapping protein expression in tissue sections with high spatial resolution due to the limited overall sensitivity of conventional workflows. Here we report an integrated and automated method to advance spatially resolved proteomics by seamlessly coupling laser capture microdissection (LCM) with a recently developed nanoliter-scale sample preparation system termed nanoPOTS (Nanodroplet Processing in One pot for Trace Samples). The workflow is enabled by prepopulating nanowells with DMSO, which serves as a sacrificial capture liquid for microdissected tissues. The DMSO droplets efficiently collect laser-pressure catapulted LCM tissues as small as 20 µm in diameter with success rates >87%. We also demonstrate that tissue treatment with DMSO can significantly improve proteome coverage, likely due to its ability to dissolve lipids from tissue and enhance protein extraction efficiency. The LCM-nanoPOTS platform was able to identify 180, 695, and 1827 protein groups on average from 12-µm-thick rat brain cortex tissue sections with diameters of 50, 100, and 200 µm, respectively. We also analyzed 100-µm-diameter sections corresponding to 10-18 cells from three different regions of rat brain and comparatively quantified ~1000 proteins, demonstrating the potential utility for high-resolution spatially resolved mapping of protein expression in tissues. Published under license by The American Society for Biochemistry and Molecular Biology, Inc.

Experimental measurement of spatially resolved electron density in a filament of a pulsed positive streamer discharge in water

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wen, Xiao Qiong; Niu, Zhi Wen; Ren, Chun-Sheng

2015-06-29

By combining a high-speed frame camera with a monochromator, the spatially resolved optical emission spectrum of hydrogen α line in a single filament of a pulsed positive streamer discharge in water has been experimentally measured. The spatially resolved electron densities in a single filament of a pulsed positive streamer discharge in water with a conductivity of 200 μS/cm were investigated. During the experiment, the average energy per pulse of discharge was 90.6 ± 13.6 mJ. The results show that the electron density in the streamer filament is 10{sup 17–18}/cm{sup 3}, and present a decreasing tendency along the axial direction of the streamer filamentmore » with increasing distance from the tip of the anode.« less

Prior-knowledge Fitting of Accelerated Five-dimensional Echo Planar J-resolved Spectroscopic Imaging: Effect of Nonlinear Reconstruction on Quantitation.

PubMed

Iqbal, Zohaib; Wilson, Neil E; Thomas, M Albert

2017-07-24

1 H Magnetic Resonance Spectroscopic imaging (SI) is a powerful tool capable of investigating metabolism in vivo from mul- tiple regions. However, SI techniques are time consuming, and are therefore difficult to implement clinically. By applying non-uniform sampling (NUS) and compressed sensing (CS) reconstruction, it is possible to accelerate these scans while re- taining key spectral information. One recently developed method that utilizes this type of acceleration is the five-dimensional echo planar J-resolved spectroscopic imaging (5D EP-JRESI) sequence, which is capable of obtaining two-dimensional (2D) spectra from three spatial dimensions. The prior-knowledge fitting (ProFit) algorithm is typically used to quantify 2D spectra in vivo, however the effects of NUS and CS reconstruction on the quantitation results are unknown. This study utilized a simulated brain phantom to investigate the errors introduced through the acceleration methods. Errors (normalized root mean square error >15%) were found between metabolite concentrations after twelve-fold acceleration for several low concentra- tion (<2 mM) metabolites. The Cramér Rao lower bound% (CRLB%) values, which are typically used for quality control, were not reflective of the increased quantitation error arising from acceleration. Finally, occipital white (OWM) and gray (OGM) human brain matter were quantified in vivo using the 5D EP-JRESI sequence with eight-fold acceleration.

REMOVING BIASES IN RESOLVED STELLAR MASS MAPS OF GALAXY DISKS THROUGH SUCCESSIVE BAYESIAN MARGINALIZATION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Martínez-García, Eric E.; González-Lópezlira, Rosa A.; Bruzual A, Gustavo

2017-01-20

Stellar masses of galaxies are frequently obtained by fitting stellar population synthesis models to galaxy photometry or spectra. The state of the art method resolves spatial structures within a galaxy to assess the total stellar mass content. In comparison to unresolved studies, resolved methods yield, on average, higher fractions of stellar mass for galaxies. In this work we improve the current method in order to mitigate a bias related to the resolved spatial distribution derived for the mass. The bias consists in an apparent filamentary mass distribution and a spatial coincidence between mass structures and dust lanes near spiral arms.more » The improved method is based on iterative Bayesian marginalization, through a new algorithm we have named Bayesian Successive Priors (BSP). We have applied BSP to M51 and to a pilot sample of 90 spiral galaxies from the Ohio State University Bright Spiral Galaxy Survey. By quantitatively comparing both methods, we find that the average fraction of stellar mass missed by unresolved studies is only half what previously thought. In contrast with the previous method, the output BSP mass maps bear a better resemblance to near-infrared images.« less

Simulations of the temporal and spatial resolution for a compact time-resolved electron diffractometer

NASA Astrophysics Data System (ADS)

Robinson, Matthew S.; Lane, Paul D.; Wann, Derek A.

2016-02-01

A novel compact electron gun for use in time-resolved gas electron diffraction experiments has recently been designed and commissioned. In this paper we present and discuss the extensive simulations that were performed to underpin the design in terms of the spatial and temporal qualities of the pulsed electron beam created by the ionisation of a gold photocathode using a femtosecond laser. The response of the electron pulses to a solenoid lens used to focus the electron beam has also been studied. The simulated results show that focussing the electron beam affects the overall spatial and temporal resolution of the experiment in a variety of ways, and that factors that improve the resolution of one parameter can often have a negative effect on the other. A balance must, therefore, be achieved between spatial and temporal resolution. The optimal experimental time resolution for the apparatus is predicted to be 416 fs for studies of gas-phase species, while the predicted spatial resolution of better than 2 nm-1 compares well with traditional time-averaged electron diffraction set-ups.

Last millennium Northern Hemisphere summer temperatures from tree rings: Part II, spatially resolved reconstructions

NASA Astrophysics Data System (ADS)

Anchukaitis, Kevin J.; Wilson, Rob; Briffa, Keith R.; Büntgen, Ulf; Cook, Edward R.; D'Arrigo, Rosanne; Davi, Nicole; Esper, Jan; Frank, David; Gunnarson, Björn E.; Hegerl, Gabi; Helama, Samuli; Klesse, Stefan; Krusic, Paul J.; Linderholm, Hans W.; Myglan, Vladimir; Osborn, Timothy J.; Zhang, Peng; Rydval, Milos; Schneider, Lea; Schurer, Andrew; Wiles, Greg; Zorita, Eduardo

2017-05-01

Climate field reconstructions from networks of tree-ring proxy data can be used to characterize regional-scale climate changes, reveal spatial anomaly patterns associated with atmospheric circulation changes, radiative forcing, and large-scale modes of ocean-atmosphere variability, and provide spatiotemporal targets for climate model comparison and evaluation. Here we use a multiproxy network of tree-ring chronologies to reconstruct spatially resolved warm season (May-August) mean temperatures across the extratropical Northern Hemisphere (40-90°N) using Point-by-Point Regression (PPR). The resulting annual maps of temperature anomalies (750-1988 CE) reveal a consistent imprint of volcanism, with 96% of reconstructed grid points experiencing colder conditions following eruptions. Solar influences are detected at the bicentennial (de Vries) frequency, although at other time scales the influence of insolation variability is weak. Approximately 90% of reconstructed grid points show warmer temperatures during the Medieval Climate Anomaly when compared to the Little Ice Age, although the magnitude varies spatially across the hemisphere. Estimates of field reconstruction skill through time and over space can guide future temporal extension and spatial expansion of the proxy network.

Quantitative evaluation of legacy phosphorus and its spatial distribution.

PubMed

Lou, Hezhen; Zhao, Changsen; Yang, Shengtian; Shi, Liuhua; Wang, Yue; Ren, Xiaoyu; Bai, Juan

2018-04-01

A phosphorus resource crisis threatens the security of global crop production, especially in developing countries like China and Brazil. Legacy phosphorus (legacy-P), which is left behind in agricultural soil by over-fertilization, can help address this issue as a new resource in the soil phosphorus pool. However, issues involved with calculating and defining the spatial distribution of legacy-P hinder its future utilization. To resolve these issues, this study applied remote sensing and ecohydrological modeling to precisely quantify legacy-P and define its spatial distribution in China's Sanjiang Plain from 2000 to 2014. The total legacy-P in the study area was calculated as 579,090 t with an annual average of 38,600 t; this comprises 51.83% of the phosphorus fertilizer applied annually. From 2000 to 2014, the annual amount of legacy-P increased by more than 3.42-fold, equivalent to a 2460-ton increase each year. The spatial distribution of legacy-P showed heterogeneity and agglomeration in this area, with peaks in cultivated land experiencing long-term agricultural development. This study supplies a new approach to finding legacy-P in soil as a precondition for future utilization. Once its spatial distribution is known, legacy-P can be better utilized in agriculture to help alleviate the phosphorus resource crisis. Copyright © 2018 Elsevier Ltd. All rights reserved.

Spatially Resolved Quantification of Chromatin Condensation through Differential Local Rheology in Cell Nuclei Fluorescence Lifetime Imaging

PubMed Central

Spagnol, Stephen T.; Dahl, Kris Noel

2016-01-01

The linear sequence of DNA encodes access to the complete set of proteins that carry out cellular functions. Yet, much of the functionality appropriate for each cell is nested within layers of dynamic regulation and organization, including a hierarchy of chromatin structural states and spatial arrangement within the nucleus. There remain limitations in our understanding of gene expression within the context of nuclear organization from an inability to characterize hierarchical chromatin organization in situ. Here we demonstrate the use of fluorescence lifetime imaging microscopy (FLIM) to quantify and spatially resolve chromatin condensation state using cell-permeable, DNA-binding dyes (Hoechst 33342 and PicoGreen). Through in vitro and in situ experiments we demonstrate the sensitivity of fluorescence lifetime to condensation state through the mechanical effects that accompany the structural changes and are reflected through altered viscosity. The establishment of FLIM for resolving and quantifying chromatin condensation state opens the door for single-measurement mechanical studies of the nucleus and for characterizing the role of genome structure and organization in nuclear processes that accompany physiological and pathological changes. PMID:26765322

Spatially resolved single crystal x-ray spectropolarimetry of wire array z-pinch plasmas

NASA Astrophysics Data System (ADS)

Wallace, M. S.; Haque, S.; Neill, P.; Pereira, N. R.; Presura, R.

2018-01-01

A recently developed single-crystal x-ray spectropolarimeter has been used to record paired sets of polarization-dependent and axially resolved x-ray spectra emitted by wire array z-pinches. In this measurement, two internal planes inside a suitable crystal diffract the x-rays into two perpendicular directions that are normal to each other, thereby separating incident x-rays into their linearly polarized components. This paper gives considerations for fielding the instrument on extended sources. Results from extended sources are difficult to interpret because generally the incident x-rays are not separated properly by the crystal. This difficulty is mitigated by using a series of collimating slits to select incident x-rays that propagate in a plane of symmetry between the polarization-splitting planes. The resulting instrument and some of the spatially resolved polarized x-ray spectra recorded for a 1-MA aluminum wire array z-pinch at the Nevada Terawatt Facility at the University of Nevada, Reno will be presented.

Spatially resolved single crystal x-ray spectropolarimetry of wire array z-pinch plasmas.

PubMed

Wallace, M S; Haque, S; Neill, P; Pereira, N R; Presura, R

2018-01-01

A recently developed single-crystal x-ray spectropolarimeter has been used to record paired sets of polarization-dependent and axially resolved x-ray spectra emitted by wire array z-pinches. In this measurement, two internal planes inside a suitable crystal diffract the x-rays into two perpendicular directions that are normal to each other, thereby separating incident x-rays into their linearly polarized components. This paper gives considerations for fielding the instrument on extended sources. Results from extended sources are difficult to interpret because generally the incident x-rays are not separated properly by the crystal. This difficulty is mitigated by using a series of collimating slits to select incident x-rays that propagate in a plane of symmetry between the polarization-splitting planes. The resulting instrument and some of the spatially resolved polarized x-ray spectra recorded for a 1-MA aluminum wire array z-pinch at the Nevada Terawatt Facility at the University of Nevada, Reno will be presented.

Quantitation of MRI sensitivity to quasi-monodisperse microbubble contrast agents for spatially resolved manometry.

PubMed

Bencsik, Martin; Al-Rwaili, Amgad; Morris, Robert; Fairhurst, David J; Mundell, Victoria; Cave, Gareth; McKendry, Jonathan; Evans, Stephen

2013-11-01

The direct in-vivo measurement of fluid pressure cannot be achieved with MRI unless it is done with the contribution of a contrast agent. No such contrast agents are currently available commercially, whilst those demonstrated previously only produced qualitative results due to their broad size distribution. Our aim is to quantitate then model the MR sensitivity to the presence of quasi-monodisperse microbubble populations. Lipid stabilised microbubble populations with mean radius 1.2 ± 0.8 μm have been produced by mechanical agitation. Contrast agents with increasing volume fraction of bubbles up to 4% were formed and the contribution the bubbles bring to the relaxation rate was quantitated. A periodic pressure change was also continuously applied to the same contrast agent, until MR signal changes were only due to bubble radius change and not due to a change in bubble density. The MR data compared favourably with the prediction of an improved numerical simulation. An excellent MR sensitivity of 23 % bar(-1) has been demonstrated. This work opens up the possibility of generating microbubble preparations tailored to specific applications with optimised MR sensitivity, in particular MRI based in-vivo manometry. Copyright © 2012 Wiley Periodicals, Inc.

Absolute Spatially- and Temporally-Resolved Optical Emission Measurements of rf Glow Discharges in Argon

PubMed Central

Djurović, S.; Roberts, J. R.; Sobolewski, M. A.; Olthoff, J. K.

1993-01-01

Spatially- and temporally-resolved measurements of optical emission intensities are presented from rf discharges in argon over a wide range of pressures (6.7 to 133 Pa) and applied rf voltages (75 to 200 V). Results of measurements of emission intensities are presented for both an atomic transition (Ar I, 750.4 nm) and an ionic transition (Ar II, 434.8 nm). The absolute scale of these optical emissions has been determined by comparison with the optical emission from a calibrated standard lamp. All measurements were made in a well-defined rf reactor. They provide detailed characterization of local time-resolved plasma conditions suitable for the comparison with results from other experiments and theoretical models. These measurements represent a new level of detail in diagnostic measurements of rf plasmas, and provide insight into the electron transport properties of rf discharges. PMID:28053464

Tracing Galactic Outflows to the Source: Spatially Resolved Feedback in M83 with COS

NASA Astrophysics Data System (ADS)

Aloisi, Alessandra

2016-10-01

Star-formation (SF) feedback plays a vital role in shaping galaxy properties, but there are many open questions about how this feedback is created, propagated, and felt by galaxies. SF-driven feedback can be observationally constrained with rest-frame UV absorption-line spectroscopy that accesses a range of powerful gas density and kinematic diagnostics. Studies at both high and low redshift show clear evidence for large-scale outflows in star-forming galaxies that scale with galaxy SF rate. However, by sampling one sightline or the galaxy as a whole, these studies are not tailored to reveal how the large-scale outflows develop from their ultimate sources at the scale of individual SF regions. We propose the first spatially-resolved COS G130M/G160M (1130-1800 A) study of the ISM in the nearby (4.6 Mpc) face-on spiral starburst M83 using individual young star clusters as background sources. This is the first down-the-barrel study where blueshifted absorptions can be identified directly with outflowing gas in a spatially resolved fashion. The kpc-scale flows sampled by the COS pointings will be anchored to the properties of the large-scale (10-100 kpc) flows thanks to the wealth of multi-wavelength observations of M83 from X-ray to radio. A comparison of COS data with mock spectra from constrained simulations of spiral galaxies with FIRE (Feedback In Realistic Environments; a code with unprecedented 1-100 pc spatial resolution and self-consistent treatments of stellar feedback) will provide an important validation of these simulations and will supply the community with a powerful and well-tested tool for galaxy formation predictions applicable to all redshifts.

Spatially-resolved aircraft-based quantification of methane emissions from the Fayetteville Shale Gas Play

NASA Astrophysics Data System (ADS)

Schwietzke, S.; Petron, G.; Conley, S. A.; Karion, A.; Tans, P. P.; Wolter, S.; King, C. W.; White, A. B.; Coleman, T.; Bianco, L.; Schnell, R. C.

2016-12-01

Confidence in basin scale oil and gas industry related methane (CH4) emission estimates hinges on an in-depth understanding, objective evaluation, and continued improvements of both top-down (e.g. aircraft measurement based) and bottom-up (e.g. emission inventories using facility- and/or component-level measurements) approaches. Systematic discrepancies of CH4 emission estimates between both approaches in the literature have highlighted research gaps. This paper is part of a more comprehensive study to expand and improve this reconciliation effort for a US dry shale gas play. This presentation will focus on refinements of the aircraft mass balance method to reduce the number of potential methodological biases (e.g. data and methodology). The refinements include (i) an in-depth exploration of the definition of upwind conditions and their impact on calculated downwind CH4 enhancements and total CH4 emissions, (ii) taking into account small but non-zero vertical and horizontal wind gradients in the boundary layer, and (iii) characterizing the spatial distribution of CH4 emissions in the study area using aircraft measurements. For the first time to our knowledge, we apply the aircraft mass balance method to calculate spatially resolved total CH4 emissions for 10 km x 60 km sub-regions within the study area. We identify higher-emitting sub-regions and localize repeating emission patterns as well as differences between days. The increased resolution of the top-down calculation will for the first time allow for an in-depth comparison with a spatially and temporally resolved bottom-up emission estimate based on measurements, concurrent activity data and other data sources.

Enhanced spatially-resolved trace analysis using combined SIMS-single-stage AMS

NASA Astrophysics Data System (ADS)

Grabowski, K. S.; Groopman, E. E.; Fahey, A. J.

2017-11-01

Secondary ion mass spectrometry (SIMS) provides spatially resolved trace analysis of solid materials, but can be complicated by unresolved abundant molecular isobars. By adding a 300-kV single-stage accelerator mass spectrometer (SSAMS) as a detector for a Cameca ims 4f SIMS, one can measure more abundant positive ions from the SIMS while removing molecular isobars, thus improving very low abundance trace element and isotope analysis. This paper describes important features and capabilities of such an integrated system at the Naval Research Laboratory using charge state +1 ions. Transmission loss is compared to molecule destruction as gas flow to the molecule-destruction cell increases. As most measurements tolerate more modest abundance sensitivities than for 14C analysis, a lower gas flow is acceptable, so good transmission of 20-50% for ions of interest can be maintained for a broad range of ion masses. This new instrument has measured isotope ratios for uranium, lead, rare earths, and other elements from particulates and localized regions, with molecule destruction enabling the measurement at low SIMS mass resolving power and thus high transmission, as examples will show. This new and world-unique instrument provides improved capabilities for applications in nuclear and other forensics, geochemistry, cosmochemistry, and the development of optical, electronic, multifunctional, and structural materials.

Reduced short term memory in congenital adrenal hyperplasia (CAH) and its relationship to spatial and quantitative performance.

PubMed

Collaer, Marcia L; Hindmarsh, Peter C; Pasterski, Vickie; Fane, Briony A; Hines, Melissa

2016-02-01

Girls and women with classical congenital adrenal hyperplasia (CAH) experience elevated androgens prenatally and show increased male-typical development for certain behaviors. Further, individuals with CAH receive glucocorticoid (GC) treatment postnatally, and this GC treatment could have negative cognitive consequences. We investigated two alternative hypotheses, that: (a) early androgen exposure in females with CAH masculinizes (improves) spatial perception and quantitative abilities at which males typically outperform females, or (b) CAH is associated with performance decrements in these domains, perhaps due to reduced short-term-memory (STM). Adolescent and adult individuals with CAH (40 female and 29 male) were compared with relative controls (29 female and 30 male) on spatial perception and quantitative abilities as well as on Digit Span (DS) to assess STM and on Vocabulary to assess general intelligence. Females with CAH did not perform better (more male-typical) on spatial perception or quantitative abilities than control females, failing to support the hypothesis of cognitive masculinization. Rather, in the sample as a whole individuals with CAH scored lower on spatial perception (p ≤ .009), a quantitative composite (p ≤ .036), and DS (p ≤ .001), despite no differences in general intelligence. Separate analyses of adolescent and adult participants suggested the spatial and quantitative effects might be present only in adult patients with CAH; however, reduced DS performance was found in patients with CAH regardless of age group. Separate regression analyses showed that DS predicted both spatial perception and quantitative performance (both p ≤ .001), when age, sex, and diagnosis status were controlled. Thus, reduced STM in CAH patients versus controls may have more general cognitive consequences, potentially reducing spatial perception and quantitative skills. Although hyponatremia or other aspects of salt-wasting crises or additional hormone

Multi-scale spectrally resolved quantitative fluorescence imaging system: towards neurosurgical guidance in glioma resection

NASA Astrophysics Data System (ADS)

Xie, Yijing; Thom, Maria; Miserocchi, Anna; McEvoy, Andrew W.; Desjardins, Adrien; Ourselin, Sebastien; Vercauteren, Tom

2017-02-01

In glioma resection surgery, the detection of tumour is often guided by using intraoperative fluorescence imaging notably with 5-ALA-PpIX, providing fluorescent contrast between normal brain tissue and the gliomas tissue to achieve improved tumour delineation and prolonged patient survival compared with the conventional white-light guided resection. However, the commercially available fluorescence imaging system relies on surgeon's eyes to visualise and distinguish the fluorescence signals, which unfortunately makes the resection subjective. In this study, we developed a novel multi-scale spectrally-resolved fluorescence imaging system and a computational model for quantification of PpIX concentration. The system consisted of a wide-field spectrally-resolved quantitative imaging device and a fluorescence endomicroscopic imaging system enabling optical biopsy. Ex vivo animal tissue experiments as well as human tumour sample studies demonstrated that the system was capable of specifically detecting the PpIX fluorescent signal and estimate the true concentration of PpIX in brain specimen.

A spatially resolved radio spectral index study of the dwarf irregular galaxy NGC 1569

NASA Astrophysics Data System (ADS)

Westcott, Jonathan; Brinks, Elias; Hindson, Luke; Beswick, Robert; Heesen, Volker

2018-04-01

We study the resolved radio continuum spectral energy distribution of the dwarf irregular galaxy, NGC 1569, on a beam-by-beam basis to isolate and study its spatially resolved radio emission characteristics. Utilizing high-quality NRAO Karl G. Jansky Very Large Array observations that densely sample the 1-34 GHz frequency range, we adopt a Bayesian fitting procedure, where we use H α emission that has not been corrected for extinction as a prior, to produce maps of how the separated thermal emission, non-thermal emission, and non-thermal spectral index vary across NGC 1569's main disc. We find a higher thermal fraction at 1 GHz than is found in spiral galaxies (26^{+2}_{-3} {per cent}) and find an average non-thermal spectral index α = -0.53 ± 0.02, suggesting that a young population of cosmic ray electrons is responsible for the observed non-thermal emission. By comparing our recovered map of the thermal radio emission with literature H α maps, we estimate the total reddening along the line of sight to NGC 1569 to be E(B - V) = 0.49 ± 0.05, which is in good agreement with other literature measurements. Spatial variations in the reddening indicate that a significant portion of the total reddening is due to internal extinction within NGC 1569.

New quantitative approaches reveal the spatial preference of nuclear compartments in mammalian fibroblasts.

PubMed

Weston, David J; Russell, Richard A; Batty, Elizabeth; Jensen, Kirsten; Stephens, David A; Adams, Niall M; Freemont, Paul S

2015-03-06

The nuclei of higher eukaryotic cells display compartmentalization and certain nuclear compartments have been shown to follow a degree of spatial organization. To date, the study of nuclear organization has often involved simple quantitative procedures that struggle with both the irregularity of the nuclear boundary and the problem of handling replicate images. Such studies typically focus on inter-object distance, rather than spatial location within the nucleus. The concern of this paper is the spatial preference of nuclear compartments, for which we have developed statistical tools to quantitatively study and explore nuclear organization. These tools combine replicate images to generate 'aggregate maps' which represent the spatial preferences of nuclear compartments. We present two examples of different compartments in mammalian fibroblasts (WI-38 and MRC-5) that demonstrate new knowledge of spatial preference within the cell nucleus. Specifically, the spatial preference of RNA polymerase II is preserved across normal and immortalized cells, whereas PML nuclear bodies exhibit a change in spatial preference from avoiding the centre in normal cells to exhibiting a preference for the centre in immortalized cells. In addition, we show that SC35 splicing speckles are excluded from the nuclear boundary and localize throughout the nucleoplasm and in the interchromatin space in non-transformed WI-38 cells. This new methodology is thus able to reveal the effect of large-scale perturbation on spatial architecture and preferences that would not be obvious from single cell imaging.

Echo-Planar Imaging-Based, J-Resolved Spectroscopic Imaging for Improved Metabolite Detection in Prostate Cancer

DTIC Science & Technology

2016-12-01

tiple dimensions (20). Hu et al. employed pseudo-random phase-encoding blips during the EPSI readout to create nonuniform sampling along the spatial...resolved MRSI with Nonuniform Undersampling and Compressed Sensing 514 30.5 Prior-knowledge Fitting for Metabolite Quantitation 515 30.6 Future Directions... NONUNIFORM UNDERSAMPLING AND COMPRESSED SENSING Nonuniform undersampling (NUS) of k-space and subsequent reconstruction using compressed sensing (CS

Maximizing the Biochemical Resolving Power of Fluorescence Microscopy

PubMed Central

Esposito, Alessandro; Popleteeva, Marina; Venkitaraman, Ashok R.

2013-01-01

Most recent advances in fluorescence microscopy have focused on achieving spatial resolutions below the diffraction limit. However, the inherent capability of fluorescence microscopy to non-invasively resolve different biochemical or physical environments in biological samples has not yet been formally described, because an adequate and general theoretical framework is lacking. Here, we develop a mathematical characterization of the biochemical resolution in fluorescence detection with Fisher information analysis. To improve the precision and the resolution of quantitative imaging methods, we demonstrate strategies for the optimization of fluorescence lifetime, fluorescence anisotropy and hyperspectral detection, as well as different multi-dimensional techniques. We describe optimized imaging protocols, provide optimization algorithms and describe precision and resolving power in biochemical imaging thanks to the analysis of the general properties of Fisher information in fluorescence detection. These strategies enable the optimal use of the information content available within the limited photon-budget typically available in fluorescence microscopy. This theoretical foundation leads to a generalized strategy for the optimization of multi-dimensional optical detection, and demonstrates how the parallel detection of all properties of fluorescence can maximize the biochemical resolving power of fluorescence microscopy, an approach we term Hyper Dimensional Imaging Microscopy (HDIM). Our work provides a theoretical framework for the description of the biochemical resolution in fluorescence microscopy, irrespective of spatial resolution, and for the development of a new class of microscopes that exploit multi-parametric detection systems. PMID:24204821

Assessment of craniospinal arteriovenous malformations at 3T with highly temporally and highly spatially resolved contrast-enhanced MR angiography.

PubMed

Saleh, R S; Lohan, D G; Villablanca, J P; Duckwiler, G; Kee, S T; Finn, J P

2008-05-01

Patients with arteriovenous malformation (AVM) are known to have an elevated risk of complications with conventional catheter angiography (CCA) but nonetheless require monitoring of hemodynamics. Thus, we aimed to evaluate both anatomy and hemodynamics in patients with AVM noninvasively by using contrast-enhanced MR angiography (CE-MRA) at 3T and to compare the results with CCA. Institutional review board approval and informed consent were obtained for this Health Insurance Portability and Accountability Act-compliant study. Twenty control subjects without vascular malformation (6 men, 18-70 years of age) and 10 patients with AVMs (6 men, 20-74 years of age) underwent supra-aortic time-resolved and high-spatial-resolution CE-MRA at 3T. Large-field-of-view coronal acquisitions extending from the root of the aorta to the cranial vertex were obtained for both MRA techniques. Image quality was assessed by 2 specialized radiologists by using a 4-point scale. AVM characteristics and nidus size were evaluated by using both CE-MRA and CCA in all patients. In patients, 96.6% (319/330) of arterial segments on high-spatial-resolution MRA and 87.7% (272/310) of arterial segments on time-resolved MRA were graded excellent/good. MRA showed 100% specificity for detecting feeding arteries and venous drainage (n = 8) and complete obliteration of the AVM in 2 cases (concordance with CCA). Nidus diameters measured by both MRA and CCA resulted in a very strong correlation (r = 0.99) with a mild overestimation by MRA (0.10 cm by using the Bland-Altman plot). By combining highly temporally resolved and highly spatially resolved MRA at 3T as complementary studies, one can assess vascular anatomy and hemodynamics noninvasively in patients with AVM.

Spatially resolved density and ionization measurements of shocked foams using x-ray fluorescence

DOE Office of Scientific and Technical Information (OSTI.GOV)

MacDonald, M. J.; Keiter, P. A.; Montgomery, D. S.

2016-09-28

We present experiments at the Trident laser facility demonstrating the use of x-ray fluorescence (XRF) to simultaneously measure density, ionization state populations, and electron temperature in shocked foams. An imaging x-ray spectrometer obtained spatially resolved measurements of Ti K-α emission. Density profiles were measured from K-α intensity. Ti ionization state distributions and electron temperatures were inferred by fitting K-α spectra to spectra from CRETIN simulations. This work shows that XRF provides a powerful tool to complement other diagnostics to make equation of state measurements of shocked materials containing a suitable tracer element.

Spatially Resolved Spectroscopy and Coronagraphic Imaging of the TW Hydrae Circumstellar Disk

NASA Astrophysics Data System (ADS)

Roberge, Aki; Weinberger, Alycia J.; Malumuth, Eliot M.

2005-04-01

We present the first spatially resolved spectrum of scattered light from the TW Hydrae protoplanetary disk. This nearly face-on disk is optically thick, surrounding a classical T Tauri star in the nearby 10 Myr old TW Hya association. The spectrum was taken with the Hubble Space Telescope (HST) STIS CCD, providing resolution R~360 over the wavelength range 5250-10300 Å. Spatially resolved spectroscopy of circumstellar disks is difficult because of the high contrast ratio between the bright star and faint disk. Our novel observations provide optical spectra of scattered light from the disk between 40 and 155 AU from the star. The scattered light has the same color as the star (gray scattering) at all radii except the innermost region. This likely indicates that the scattering dust grains are larger than about 1 μm all the way out to large radii. From the spectroscopic data, we also obtained radial profiles of the integrated disk brightness at two position angles, over almost the same region as previously observed in HST WFPC2 and NICMOS coronagraphic images (35 to 173 AU from the star). The profiles have the same shape as the earlier ones, but show a small azimuthal asymmetry in the disk not previously noted. Our STIS broadband coronagraphic images of TW Hya confirm the reality of this asymmetry, and show that the disk surface brightness inside 140 AU has a sinusoidal dependence on azimuthal angle. The maximum brightness occurs at a position angle of 233.6d+/-5.7d east of north. This might be caused by the combination of forward scattering and an increase in inclination in the inner region of the disk, suggesting that the TW Hya disk has a warp like that seen in the β Pictoris debris disk.

Spatially-resolved magnetic resonance study of the dissolution interface between soaps and water

NASA Astrophysics Data System (ADS)

Ciampi, E.; Goerke, U.; McDonald, P. J.; Chambers, J. G.; Newling, B.

2002-06-01

The developing interfacial region between a soap bar and water has been studied using a suite of spatially resolved NMR techniques. Stray field imaging (STRAFI) allowed the dynamics of water ingress into a shop-bought, commercial soap to be followed. A simplistic analysis of the data shows the ingress to be a Fickian process (∝t1/2) in the first 4 h. The T2 contrast employed in the STRAFI method is not sufficient to resolve detail of the mesophase formation at the interface. However, double quantum filtered 2H spectroscopy at different positions in the interfacial region allowed water concentration (and mesophase distribution) to be mapped over the first 120 h of dissolution. A simple model shows good agreement with the water concentration data. In the isotropic soap solution above the interfacial region, J-cyclic cross polarization was used to selectively interrogate the CH2 1H of the soap alkyl chains and, in combination with a pulsed field gradient measurement of self-diffusion, suggests a micellar solution in which the hydrodynamic radius of the micelles is ~5nm.

The Fossil Record of Black Hole Seeds, with Spatially Resolved Spectroscopy

NASA Astrophysics Data System (ADS)

Trump, Jonathan R.; CANDELS, 3D-HST

2016-01-01

I will present the first robust measurement of black hole occupation over a wide range of host galaxy mass (8spatially resolved spectroscopy, which reliably distinguishes a nuclear AGN from extended star formation and largely avoids the star-formation dilution bias plaguing traditional low-mass AGN selection. The observations suggest bimodal seed formation: while many low-mass galaxies host massive black holes, their black hole occupation is ~10% that of massive galaxies. The measured black hole occupation qualitatively agrees with theoretical models of black hole formation, with massive direct-collapse seeds forming only in massive halos and black hole formation confined to lower-mass Pop III remnants in small halos.

Spatial access priority mapping (SAPM) with fishers: a quantitative GIS method for participatory planning.

PubMed

Yates, Katherine L; Schoeman, David S

2013-01-01

Spatial management tools, such as marine spatial planning and marine protected areas, are playing an increasingly important role in attempts to improve marine management and accommodate conflicting needs. Robust data are needed to inform decisions among different planning options, and early inclusion of stakeholder involvement is widely regarded as vital for success. One of the biggest stakeholder groups, and the most likely to be adversely impacted by spatial restrictions, is the fishing community. In order to take their priorities into account, planners need to understand spatial variation in their perceived value of the sea. Here a readily accessible, novel method for quantitatively mapping fishers' spatial access priorities is presented. Spatial access priority mapping, or SAPM, uses only basic functions of standard spreadsheet and GIS software. Unlike the use of remote-sensing data, SAPM actively engages fishers in participatory mapping, documenting rather than inferring their priorities. By so doing, SAPM also facilitates the gathering of other useful data, such as local ecological knowledge. The method was tested and validated in Northern Ireland, where over 100 fishers participated in a semi-structured questionnaire and mapping exercise. The response rate was excellent, 97%, demonstrating fishers' willingness to be involved. The resultant maps are easily accessible and instantly informative, providing a very clear visual indication of which areas are most important for the fishers. The maps also provide quantitative data, which can be used to analyse the relative impact of different management options on the fishing industry and can be incorporated into planning software, such as MARXAN, to ensure that conservation goals can be met at minimum negative impact to the industry. This research shows how spatial access priority mapping can facilitate the early engagement of fishers and the ready incorporation of their priorities into the decision-making process

A Quantitative Three-Dimensional Image Analysis Tool for Maximal Acquisition of Spatial Heterogeneity Data.

PubMed

Allenby, Mark C; Misener, Ruth; Panoskaltsis, Nicki; Mantalaris, Athanasios

2017-02-01

Three-dimensional (3D) imaging techniques provide spatial insight into environmental and cellular interactions and are implemented in various fields, including tissue engineering, but have been restricted by limited quantification tools that misrepresent or underutilize the cellular phenomena captured. This study develops image postprocessing algorithms pairing complex Euclidean metrics with Monte Carlo simulations to quantitatively assess cell and microenvironment spatial distributions while utilizing, for the first time, the entire 3D image captured. Although current methods only analyze a central fraction of presented confocal microscopy images, the proposed algorithms can utilize 210% more cells to calculate 3D spatial distributions that can span a 23-fold longer distance. These algorithms seek to leverage the high sample cost of 3D tissue imaging techniques by extracting maximal quantitative data throughout the captured image.

Mid-infrared laser absorption tomography for quantitative 2D thermochemistry measurements in premixed jet flames

NASA Astrophysics Data System (ADS)

Wei, Chuyu; Pineda, Daniel I.; Paxton, Laurel; Egolfopoulos, Fokion N.; Spearrin, R. Mitchell

2018-06-01

A tomographic laser absorption spectroscopy technique, utilizing mid-infrared light sources, is presented as a quantitative method to spatially resolve species and temperature profiles in small-diameter reacting flows relevant to combustion systems. Here, tunable quantum and interband cascade lasers are used to spectrally resolve select rovibrational transitions near 4.98 and 4.19 μm to measure CO and {CO2}, respectively, as well as their vibrational temperatures, in piloted premixed jet flames. Signal processing methods are detailed for the reconstruction of axial and radial profiles of thermochemical structure in a canonical ethylene-air jet flame. The method is further demonstrated to quantitatively distinguish between different turbulent flow conditions.

Spatially-resolved probing of biological phantoms by point-radiance spectroscopy

NASA Astrophysics Data System (ADS)

Grabtchak, Serge; Palmer, Tyler J.; Whelan, William M.

2011-03-01

Interstitial fiber-optic based strategies for therapy monitoring and assessment rely on detecting treatment-induced changes in the light distribution in biological tissues. We present an optical technique to identify spectrally and spatially specific tissue chromophores in highly scattering turbid media. Typical optical sensors measure non-directional light intensity (i.e. fluence) and require fiber translation (i.e. 3-5 positions), which is difficult to implement clinically. Point radiance spectroscopy is based on directional light collection (i.e. radiance) at a single point with a side-firing fiber that can be rotated up to 360°. A side firing fiber accepts light within a well-defined solid angle thus potentially providing an improved spatial resolution. Experimental measurements were performed using an 800-μm diameter isotropic spherical diffuser coupled to a halogen light source and a 600 μm, ~43° cleaved fiber (i.e. radiance detector). The background liquid-based scattering phantom was fabricated using 1% Intralipid (i.e. scattering medium). Light was collected at 1-5° increments through 360°-segment. Gold nanoparticles, placed into a 3.5 mm diameter capillary tube were used as localized scatterers and absorbers introduced into the liquid phantom both on- and off-axis between source and detector. The localized optical inhomogeneity was detectable as an angular-resolved variation in the radiance polar plots. This technique is being investigated as a non-invasive optical modality for prostate cancer monitoring.

Spatially resolved δ13C analysis using laser ablation isotope ratio mass spectrometry

NASA Astrophysics Data System (ADS)

Moran, J.; Riha, K. M.; Nims, M. K.; Linley, T. J.; Hess, N. J.; Nico, P. S.

2014-12-01

Inherent geochemical, organic matter, and microbial heterogeneity over small spatial scales can complicate studies of carbon dynamics through soils. Stable isotope analysis has a strong history of helping track substrate turnover, delineate rhizosphere activity zones, and identifying transitions in vegetation cover, but most traditional isotope approaches are limited in spatial resolution by a combination of physical separation techniques (manual dissection) and IRMS instrument sensitivity. We coupled laser ablation sampling with isotope measurement via IRMS to enable spatially resolved analysis over solid surfaces. Once a targeted sample region is ablated the resulting particulates are entrained in a helium carrier gas and passed through a combustion reactor where carbon is converted to CO2. Cyrotrapping of the resulting CO2 enables a reduction in carrier gas flow which improves overall measurement sensitivity versus traditional, high flow sample introduction. Currently we are performing sample analysis at 50 μm resolution, require 65 ng C per analysis, and achieve measurement precision consistent with other continuous flow techniques. We will discuss applications of the laser ablation IRMS (LA-IRMS) system to microbial communities and fish ecology studies to demonstrate the merits of this technique and how similar analytical approaches can be transitioned to soil systems. Preliminary efforts at analyzing soil samples will be used to highlight strengths and limitations of the LA-IRMS approach, paying particular attention to sample preparation requirements, spatial resolution, sample analysis time, and the types of questions most conducive to analysis via LA-IRMS.

Coupling of oceanic carbon and nitrogen: A window to spatially resolved quantitative reconstruction of nitrate inventories

NASA Astrophysics Data System (ADS)

Glock, N.; Liebetrau, V.; Gorb, S.; Wallmann, K. J. G.; Erdem, Z.; Schönfeld, J.; Eisenhauer, A.

2017-12-01

Anthropogenic impact has led to a severe acceleration of the global nitrogen cycle. Every second nitrogen atom in the biosphere may now originate from anthropogenic sources such as chemical fertilizers and the burning of fossil fuels. A quantitative reconstruction of past reactive nitrogen inventories is invaluable to facilitate projections for future scenarios and calibrations for such paleoproxies should be done as long the natural signature is still visible. Here we present a first quantitative reconstruction of nitrate concentrations in intermediate water depths of the Peruvian oxygen minimum zone over the last deglaciation using the pore density in the benthic foraminiferal species Bolivina spissa. A comparison of the nitrate reconstruction to the stable carbon isotope (δ13C) record reveals a strong coupling between the carbon and nitrogen cycles. The linear correlation between δ13C and nitrate availability remained stable over the last 22,000 years, facilitating the use of δ13C records as a quantitative nitrate proxy. The combination of the pore density record with δ13C records shows an elevated oceanic nitrate inventory during the Last Glacial Maximum as compared to the Holocene. Our novel proxy approach is consistent with the results of previous δ15N-based biogeochemical modeling studies, and thus provides sound estimates of the nitrate inventory in the glacial and deglacial ocean.

Direct measurement of lateral transport in membranes by using time-resolved spatial photometry.

PubMed Central

Kapitza, H G; McGregor, G; Jacobson, K A

1985-01-01

Spatially resolving light detectors allow, with proper calibration, quantitative analysis of the variations in two-dimensional intensity distributions over time. An ultrasensitive microfluorometer was assembled by using as a detector a microchannel plate-intensified video camera. The camera was interfaced with a software-based digital video analysis system to digitize, average, and process images and to directly control the timing of the experiments to minimize exposure of the specimen to light. The detector system has been characterized to allow its use as a photometer. A major application has been to perform fluorescence recovery after photobleaching measurements by using the camera in place of a photomultiplier tube (video-FRAP) with the goal of detecting possible anisotropic diffusion or convective flow. Analysis of the data on macromolecular diffusion in homogenous aqueous glycol solutions yielded diffusion constants in agreement with previous measurements. Results on lipid probe diffusion in dimyristoylphosphatidylcholine multibilayers indicated that at temperatures above the gel-to-liquid crystalline phase transition diffusion is isotropic, and analysis of video-FRAP data yielded diffusion coefficients consistent with those measured previously by using spot photobleaching. However, lipid probes in these multibilayers held just below the main phase transition temperature exhibited markedly anisotropic diffusive fluxes when the bleaching beam was positioned proximate to domain boundaries in the P beta' phase. Lipid probes and lectin receptor complexes diffused isotropically in fibroblast surface membranes with little evidence for diffusion channeled parallel to stress fibers. A second application was to trace the time evolution of cell surface reactions such as patching. The feasibility of following, on the optical scale, the growth of individual receptor clusters induced by the ligand wheat germ agglutinin was demonstrated. PMID:3858869

Direct measurement of lateral transport in membranes by using time-resolved spatial photometry.

PubMed

Kapitza, H G; McGregor, G; Jacobson, K A

1985-06-01

Spatially resolving light detectors allow, with proper calibration, quantitative analysis of the variations in two-dimensional intensity distributions over time. An ultrasensitive microfluorometer was assembled by using as a detector a microchannel plate-intensified video camera. The camera was interfaced with a software-based digital video analysis system to digitize, average, and process images and to directly control the timing of the experiments to minimize exposure of the specimen to light. The detector system has been characterized to allow its use as a photometer. A major application has been to perform fluorescence recovery after photobleaching measurements by using the camera in place of a photomultiplier tube (video-FRAP) with the goal of detecting possible anisotropic diffusion or convective flow. Analysis of the data on macromolecular diffusion in homogenous aqueous glycol solutions yielded diffusion constants in agreement with previous measurements. Results on lipid probe diffusion in dimyristoylphosphatidylcholine multibilayers indicated that at temperatures above the gel-to-liquid crystalline phase transition diffusion is isotropic, and analysis of video-FRAP data yielded diffusion coefficients consistent with those measured previously by using spot photobleaching. However, lipid probes in these multibilayers held just below the main phase transition temperature exhibited markedly anisotropic diffusive fluxes when the bleaching beam was positioned proximate to domain boundaries in the P beta' phase. Lipid probes and lectin receptor complexes diffused isotropically in fibroblast surface membranes with little evidence for diffusion channeled parallel to stress fibers. A second application was to trace the time evolution of cell surface reactions such as patching. The feasibility of following, on the optical scale, the growth of individual receptor clusters induced by the ligand wheat germ agglutinin was demonstrated.

Spatially resolved density and ionization measurements of shocked foams using x-ray fluorescence

DOE Office of Scientific and Technical Information (OSTI.GOV)

MacDonald, M. J.; Keiter, P. A.; Montgomery, D. S.

2016-09-22

We present experiments at the Trident laser facility demonstrating the use of x-ray fluorescence (XRF) to simultaneously measure density, ionization state populations, and electron temperature in shocked foams. An imaging x-ray spectrometer was used to obtain spatially-resolved measurements of Ti K-more » $$\\alpha$$ emission. Density profiles were measured from K-$$\\alpha$$ intensity. Ti ionization state distributions and electron temperatures were inferred by fitting K-$$\\alpha$$ spectra to spectra from CRETIN simulations. This study shows that XRF provides a powerful tool to complement other diagnostics to make equation of state measurements of shocked materials containing a suitable tracer element.« less

Spatially resolved high-resolution x-ray spectroscopy of high-current plasma-focus discharges.

PubMed

Zając, S; Rzadkiewicz, J; Rosmej, O; Scholz, M; Yongtao, Zhao; Gójska, A; Paduch, M; Zielińska, E

2010-10-01

Soft x-ray emission from a Mather-type plasma-focus device (PF-1000) operated at ∼400 kJ was measured. The high density and temperature plasma were generated by the discharge in the deuterium-argon gas mixture in the modified (high-current) plasma-focus configuration. A spherically bent mica crystal spectrograph viewing the axial output of the pinch region was used to measure the x-ray spectra. Spatially resolved spectra including the characteristic x-ray lines of highly ionized Ar and continua were recorded by means of an x-ray film. The x-ray emission of PF-1000 device was studied at different areas of the pinch.

Spatially resolved high-resolution x-ray spectroscopy of high-current plasma-focus discharges

DOE Office of Scientific and Technical Information (OSTI.GOV)

ZajaPc, S.; Rzadkiewicz, J.; Scholz, M.

Soft x-ray emission from a Mather-type plasma-focus device (PF-1000) operated at {approx}400 kJ was measured. The high density and temperature plasma were generated by the discharge in the deuterium-argon gas mixture in the modified (high-current) plasma-focus configuration. A spherically bent mica crystal spectrograph viewing the axial output of the pinch region was used to measure the x-ray spectra. Spatially resolved spectra including the characteristic x-ray lines of highly ionized Ar and continua were recorded by means of an x-ray film. The x-ray emission of PF-1000 device was studied at different areas of the pinch.

Spatially resolved, in situ potential measurements through porous electrodes as applied to fuel cells.

PubMed

Hess, Katherine C; Epting, William K; Litster, Shawn

2011-12-15

We report the development and use of a microstructured electrode scaffold (MES) to make spatially resolved, in situ, electrolyte potential measurements through the thickness of a polymer electrolyte fuel cell (PEFC) electrode. This new approach uses a microfabricated apparatus to analyze the coupled transport and electrochemical phenomena in porous electrodes at the microscale. In this study, the MES allows the fuel cell to run under near-standard operating conditions, while providing electrolyte potential measurements at discrete distances through the electrode's thickness. Here we use spatial distributions of electrolyte potential to evaluate the effects of Ohmic and mass transport resistances on the through-plane reaction distribution for various operating conditions. Additionally, we use the potential distributions to estimate the ionic conductivity of the electrode. Our results indicate the in situ conductivity is higher than typically estimated for PEFC electrodes based on bulk polymer electrolyte membrane (PEM) conductivity.

Quantitative characterization of the spatial distribution of particles in materials: Application to materials processing

NASA Technical Reports Server (NTRS)

Parse, Joseph B.; Wert, J. A.

1991-01-01

Inhomogeneities in the spatial distribution of second phase particles in engineering materials are known to affect certain mechanical properties. Progress in this area has been hampered by the lack of a convenient method for quantitative description of the spatial distribution of the second phase. This study intends to develop a broadly applicable method for the quantitative analysis and description of the spatial distribution of second phase particles. The method was designed to operate on a desktop computer. The Dirichlet tessellation technique (geometrical method for dividing an area containing an array of points into a set of polygons uniquely associated with the individual particles) was selected as the basis of an analysis technique implemented on a PC. This technique is being applied to the production of Al sheet by PM processing methods; vacuum hot pressing, forging, and rolling. The effect of varying hot working parameters on the spatial distribution of aluminum oxide particles in consolidated sheet is being studied. Changes in distributions of properties such as through-thickness near-neighbor distance correlate with hot-working reduction.

Utility of spatially-resolved atmospheric pressure surface sampling and ionization techniques as alternatives to mass spectrometric imaging (MSI) in drug metabolism.

PubMed

Blatherwick, Eleanor Q; Van Berkel, Gary J; Pickup, Kathryn; Johansson, Maria K; Beaudoin, Marie-Eve; Cole, Roderic O; Day, Jennifer M; Iverson, Suzanne; Wilson, Ian D; Scrivens, James H; Weston, Daniel J

2011-08-01

Tissue distribution studies of drug molecules play an essential role in the pharmaceutical industry and are commonly undertaken using quantitative whole body autoradiography (QWBA) methods. The growing need for complementary methods to address some scientific gaps around radiography methods has led to increased use of mass spectrometric imaging (MSI) technology over the last 5 to 10 years. More recently, the development of novel mass spectrometric techniques for ambient surface sampling has redefined what can be regarded as "fit-for-purpose" for MSI in a drug metabolism and disposition arena. Together with a review of these novel alternatives, this paper details the use of two liquid microjunction (LMJ)-based mass spectrometric surface sampling technologies. These approaches are used to provide qualitative determination of parent drug in rat liver tissue slices using liquid extraction surface analysis (LESA) and to assess the performance of a LMJ surface sampling probe (LMJ-SSP) interface for quantitative assessment of parent drug in brain, liver and muscle tissue slices. An assessment of the utility of these spatially-resolved sampling methods is given, showing interdependence between mass spectrometric and QWBA methods, in particular there emerges a reason to question typical MSI workflows for drug metabolism; suggesting the expedient use of profile or region analysis may be more appropriate, rather than generating time-intensive molecular images of the entire tissue section.

Distortion correction and cross-talk compensation algorithm for use with an imaging spectrometer based spatially resolved diffuse reflectance system

NASA Astrophysics Data System (ADS)

Cappon, Derek J.; Farrell, Thomas J.; Fang, Qiyin; Hayward, Joseph E.

2016-12-01

Optical spectroscopy of human tissue has been widely applied within the field of biomedical optics to allow rapid, in vivo characterization and analysis of the tissue. When designing an instrument of this type, an imaging spectrometer is often employed to allow for simultaneous analysis of distinct signals. This is especially important when performing spatially resolved diffuse reflectance spectroscopy. In this article, an algorithm is presented that allows for the automated processing of 2-dimensional images acquired from an imaging spectrometer. The algorithm automatically defines distinct spectrometer tracks and adaptively compensates for distortion introduced by optical components in the imaging chain. Crosstalk resulting from the overlap of adjacent spectrometer tracks in the image is detected and subtracted from each signal. The algorithm's performance is demonstrated in the processing of spatially resolved diffuse reflectance spectra recovered from an Intralipid and ink liquid phantom and is shown to increase the range of wavelengths over which usable data can be recovered.

Spatially Resolved Spectroscopy of the PMS Quadruple GG Tau: Evidence for a Substellar Companion

NASA Astrophysics Data System (ADS)

White, R. J.; Ghez, A. M.; Schultz, G.; Reid, I. N.

1998-05-01

We present spatially resolved optical spectra from HST (FOS) and the Keck Telescope (HIRES & LRIS) of the components of the quadruple PMS system GG Tau. According to the latest PMS evolutionary models, the coldest component of this system, GG Tau/c B, appears to be substellar with a preliminary mass of only 50 M_J. This putative brown dwarf is especially intriguing as it shows clear signatures of accretion. The components of this quadruple, which span a wide range in mass, are used to test theoretical low mass PMS evolutionary models under the assumption that the components should be coeval.

Spatially resolved chemical imaging of individual atmospheric particles using nanoscale imaging mass spectrometry: insight into particle origin and chemistry

DOE PAGES

Ghosal, Sutapa; Weber, Peter K.; Laskin, Alexander

2014-01-14

Knowledge of the spatially resolved composition of atmospheric particles is essential for differentiating between their surface versus bulk chemistry and understanding particle reactivity and the potential environmental impact. Here, we demonstrate the application of nanometer-scale secondary ion mass spectrometry (CAMECA NanoSIMS 50 ion probe) for 3D chemical imaging of individual atmospheric particles without any sample pre-treatment, such as sectioning of particles. Use of NanoSIMS depth profile analysis enables elemental mapping of particles with nanometer spatial resolution over a broad range of particle sizes. We have used this technique to probe the spatially resolved composition of ambient particles collected during amore » field campaign in Mexico City. Particles collected during this campaign have been extensively characterized in the past using other particle analysis techniques and hence offer a unique opportunity for exploring the utility of depth-resolved chemical imaging in ambient particle research. The particles that we examined in our study include those collected during a pollution episode related to urban waste incineration as well as background particles from the same location before the episode. Particles from the pollution episode show substantial intra-particle compositional variability typical of particles resulting from multiple emission sources. In contrast, the background particles have relatively homogeneous compositions with enhanced presence of nitrogen, oxygen, and chlorine at the particle surface. We also observed the surface enhancement of nitrogen and oxygen species is consistent with the presence of surface nitrates resulting from gas–particle heterogeneous interactions and is indicative of atmospheric ageing of the particles. The results presented here illustrate 3D characterization of ambient particles for insight into their chemical history.« less

Spatially resolved chemical imaging of individual atmospheric particles using nanoscale imaging mass spectrometry: Insighs into particle origin and chemistry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ghosal, Sutapa; Weber, Peter K.; Laskin, Alexander

2014-04-21

Knowledge of the spatially-resolved composition of atmospheric particles is essential for differentiating between their surface versus bulk chemistry, understanding particle reactivity and the potential environmental impact. We demonstrate the application of nanometer-scale secondary ion mass spectrometry (Cameca NanoSIMS 50 ion probe) for 3D chemical imaging of individual atmospheric particles without any sample pre-treatment, such as the sectioning of particles. Use of NanoSIMS depth profile analysis enables elemental mapping of particles with nanometer spatial resolution over a broad of range of particle sizes. We have used this technique to probe spatially resolved composition of ambient particles collected during a field campaignmore » in Mexico City. Particles collected during this campaign have been extensively characterized in the past using other particle analysis techniques and hence offer a unique opportunity for exploring the utility of depth resolved chemical imaging in ambient particle research. 1 Particles examined in this study include those collected during a pollution episode related to urban waste incineration as well as background particles from the same location prior to the episode. Particles from the pollution episode show substantial intra-particle compositional variability typical of particles resulting from multiple emission sources. In contrast, the background particles have relatively homogeneous compositions with enhanced presence of nitrogen, oxygen and chlorine at the particle surface. The observed surface enhancement of nitrogen and oxygen species is consistent with the presence of surface nitrates resulting from gas-particle heterogeneous interactions and is indicative of atmospheric ageing of the particles. The results presented here illustrate 3D characterization of ambient particles for insights into their chemical history.« less

Spatially resolved positron annihilation spectroscopy on friction stir weld induced defects.

PubMed

Hain, Karin; Hugenschmidt, Christoph; Pikart, Philip; Böni, Peter

2010-04-01

A friction stir welded (FSW) Al alloy sample was investigated by Doppler broadening spectroscopy (DBS) of the positron annihilation line. The spatially resolved defect distribution showed that the material in the joint zone becomes completely annealed during the welding process at the shoulder of the FSW tool, whereas at the tip, annealing is prevailed by the deterioration of the material due to the tool movement. This might be responsible for the increased probability of cracking in the heat affected zone of friction stir welds. Examination of a material pairing of steel S235 and the Al alloy Silafont36 by coincident Doppler broadening spectroscopy (CDBS) indicates the formation of annealed steel clusters in the Al alloy component of the sample. The clear visibility of Fe in the CDB spectra is explained by the very efficient trapping at the interface between steel cluster and bulk.

Quantitative depth resolved microcirculation imaging with optical coherence tomography angiography (Part ΙΙ): Microvascular network imaging.

PubMed

Gao, Wanrong

2017-04-17

In this work, we review the main phenomena that have been explored in OCT angiography to image the vessels of the microcirculation within living tissues with the emphasis on how the different processing algorithms were derived to circumvent specific limitations. Parameters are then discussed that can quantitatively describe the depth-resolved microvascular network for possible clinic diagnosis applications. Finally,future directions in continuing OCT development are discussed. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Reprint Of: Enhanced spatially-resolved trace analysis using combined SIMS-single-stage AMS

NASA Astrophysics Data System (ADS)

Grabowski, K. S.; Groopman, E. E.; Fahey, A. J.

2018-01-01

Secondary ion mass spectrometry (SIMS) provides spatially resolved trace analysis of solid materials, but can be complicated by unresolved abundant molecular isobars. By adding a 300-kV single-stage accelerator mass spectrometer (SSAMS) as a detector for a Cameca ims 4f SIMS, one can measure more abundant positive ions from the SIMS while removing molecular isobars, thus improving very low abundance trace element and isotope analysis. This paper describes important features and capabilities of such an integrated system at the Naval Research Laboratory using charge state +1 ions. Transmission loss is compared to molecule destruction as gas flow to the molecule-destruction cell increases. As most measurements tolerate more modest abundance sensitivities than for 14C analysis, a lower gas flow is acceptable, so good transmission of 20-50% for ions of interest can be maintained for a broad range of ion masses. This new instrument has measured isotope ratios for uranium, lead, rare earths, and other elements from particulates and localized regions, with molecule destruction enabling the measurement at low SIMS mass resolving power and thus high transmission, as examples will show. This new and world-unique instrument provides improved capabilities for applications in nuclear and other forensics, geochemistry, cosmochemistry, and the development of optical, electronic, multifunctional, and structural materials.

A Spatially Resolving X-ray Crystal Spectrometer for Measurement of Ion-temperature and Rotation-velocity Profiles on the AlcatorC-Mod Tokamak

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hill, K. W.; Bitter, M. L.; Scott, S. D.

2009-03-24

A new spatially resolving x-ray crystal spectrometer capable of measuring continuous spatial profiles of high resolution spectra (λ/dλ > 6000) of He-like and H-like Ar Kα lines with good spatial (~1 cm) and temporal (~10 ms) resolutions has been installed on the Alcator C-Mod tokamak. Two spherically bent crystals image the spectra onto four two-dimensional Pilatus II pixel detectors. Tomographic inversion enables inference of local line emissivity, ion temperature (Ti), and toroidal plasma rotation velocity (vφ) from the line Doppler widths and shifts. The data analysis techniqu

Instrument for spatially resolved simultaneous measurements of forces and currents in particle beams

DOE Office of Scientific and Technical Information (OSTI.GOV)

Spethmann, A., E-mail: spethmann@physik.uni-kiel.de; Trottenberg, T., E-mail: trottenberg@physik.uni-kiel.de; Kersten, H., E-mail: kersten@physik.uni-kiel.de

The article presents a device for spatially resolved and simultaneous measurements of forces and currents in particle beams, especially in beams composed of ions and neutral atoms. The forces are exerted by the impinging beam particles on a plane circular conductive target plate of 20 mm diameter mounted on a pendulum with electromagnetic force compensation. The force measurement in the micronewton range is achieved by electromagnetic compensation by means of static Helmholtz coils and permanent magnets attached to the pendulum. Exemplary measurements are performed in the 1.2 keV beam of a broad beam ion source. The simultaneous measurements of forcesmore » and currents onto the same target are compared with each other and with Faraday cup measurements.« less

Noninvasive diagnostics of skin microphysical parameters based on spatially resolved diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Lisenko, S. A.; Kugeiko, M. M.

2013-01-01

The ability to determine noninvasively microphysical parameters (MPPs) of skin characteristic of malignant melanoma was demonstrated. The MPPs were the melanin content in dermis, saturation of tissue with blood vessels, and concentration and effective size of tissue scatterers. The proposed method was based on spatially resolved spectral measurements of skin diffuse reflectance and multiple regressions between linearly independent measurement components and skin MPPs. The regressions were established by modeling radiation transfer in skin with a wide variation of its MPPs. Errors in the determination of skin MPPs were estimated using fiber-optic measurements of its diffuse reflectance at wavelengths of commercially available semiconductor diode lasers (578, 625, 660, 760, and 806 nm) at source-detector separations of 0.23-1.38 mm.

Spatially and Temporally Resolved Studies of the Human Microbiome (2011 JGI User Meeting)

ScienceCinema

Knight, Rob

2018-04-26

The U.S. Department of Energy Joint Genome Institute (JGI) invited scientists interested in the application of genomics to bioenergy and environmental issues, as well as all current and prospective users and collaborators, to attend the annual DOE JGI Genomics of Energy & Environment Meeting held March 22-24, 2011 in Walnut Creek, Calif. The emphasis of this meeting was on the genomics of renewable energy strategies, carbon cycling, environmental gene discovery, and engineering of fuel-producing organisms. The meeting features presentations by leading scientists advancing these topics. Rob Knight of the University of Colorado gives a presentation on "Spatially and Temporally Resolved Studies of the Human Microbiome" at the 6th annual Genomics of Energy & Environment Meeting on March 23, 2011.

Spatially resolved quantitative mapping of thermomechanical properties and phase transition temperatures using scanning probe microscopy

DOEpatents

Jesse, Stephen; Kalinin, Sergei V; Nikiforov, Maxim P

2013-07-09

An approach for the thermomechanical characterization of phase transitions in polymeric materials (polyethyleneterephthalate) by band excitation acoustic force microscopy is developed. This methodology allows the independent measurement of resonance frequency, Q factor, and oscillation amplitude of a tip-surface contact area as a function of tip temperature, from which the thermal evolution of tip-surface spring constant and mechanical dissipation can be extracted. A heating protocol maintained a constant tip-surface contact area and constant contact force, thereby allowing for reproducible measurements and quantitative extraction of material properties including temperature dependence of indentation-based elastic and loss moduli.

SDSS-IV MaNGA: Spatially Resolved Star Formation Main Sequence and LI(N)ER Sequence

NASA Astrophysics Data System (ADS)

Hsieh, B. C.; Lin, Lihwai; Lin, J. H.; Pan, H. A.; Hsu, C. H.; Sánchez, S. F.; Cano-Díaz, M.; Zhang, K.; Yan, R.; Barrera-Ballesteros, J. K.; Boquien, M.; Riffel, R.; Brownstein, J.; Cruz-González, I.; Hagen, A.; Ibarra, H.; Pan, K.; Bizyaev, D.; Oravetz, D.; Simmons, A.

2017-12-01

We present our study on the spatially resolved Hα and M * relation for 536 star-forming and 424 quiescent galaxies taken from the MaNGA survey. We show that the star formation rate surface density ({{{Σ }}}{SFR}), derived based on the Hα emissions, is strongly correlated with the M * surface density ({{{Σ }}}* ) on kiloparsec scales for star-forming galaxies and can be directly connected to the global star-forming sequence. This suggests that the global main sequence may be a consequence of a more fundamental relation on small scales. On the other hand, our result suggests that ∼20% of quiescent galaxies in our sample still have star formation activities in the outer region with lower specific star formation rate (SSFR) than typical star-forming galaxies. Meanwhile, we also find a tight correlation between {{{Σ }}}{{H}α } and {{{Σ }}}* for LI(N)ER regions, named the resolved “LI(N)ER” sequence, in quiescent galaxies, which is consistent with the scenario that LI(N)ER emissions are primarily powered by the hot, evolved stars as suggested in the literature.

Integrated circuit-based electrochemical sensor for spatially resolved detection of redox-active metabolites in biofilms.

PubMed

Bellin, Daniel L; Sakhtah, Hassan; Rosenstein, Jacob K; Levine, Peter M; Thimot, Jordan; Emmett, Kevin; Dietrich, Lars E P; Shepard, Kenneth L

2014-01-01

Despite advances in monitoring spatiotemporal expression patterns of genes and proteins with fluorescent probes, direct detection of metabolites and small molecules remains challenging. A technique for spatially resolved detection of small molecules would benefit the study of redox-active metabolites that are produced by microbial biofilms and can affect their development. Here we present an integrated circuit-based electrochemical sensing platform featuring an array of working electrodes and parallel potentiostat channels. 'Images' over a 3.25 × 0.9 mm(2) area can be captured with a diffusion-limited spatial resolution of 750 μm. We demonstrate that square wave voltammetry can be used to detect, identify and quantify (for concentrations as low as 2.6 μM) four distinct redox-active metabolites called phenazines. We characterize phenazine production in both wild-type and mutant Pseudomonas aeruginosa PA14 colony biofilms, and find correlations with fluorescent reporter imaging of phenazine biosynthetic gene expression.

Integrated circuit-based electrochemical sensor for spatially resolved detection of redox-active metabolites in biofilms

PubMed Central

Bellin, Daniel L.; Sakhtah, Hassan; Rosenstein, Jacob K.; Levine, Peter M.; Thimot, Jordan; Emmett, Kevin; Dietrich, Lars E. P.; Shepard, Kenneth L.

2014-01-01

Despite advances in monitoring spatiotemporal expression patterns of genes and proteins with fluorescent probes, direct detection of metabolites and small molecules remains challenging. A technique for spatially resolved detection of small molecules would benefit the study of redox-active metabolites produced by microbial biofilms, which can drastically affect colony development. Here we present an integrated circuit-based electrochemical sensing platform featuring an array of working electrodes and parallel potentiostat channels. “Images” over a 3.25 × 0.9 mm area can be captured with a diffusion-limited spatial resolution of 750 μm. We demonstrate that square wave voltammetry can be used to detect, identify, and quantify (for concentrations as low as 2.6 μM) four distinct redox-active metabolites called phenazines. We characterize phenazine production in both wild-type and mutant Pseudomonas aeruginosa PA14 colony biofilms, and find correlations with fluorescent reporter imaging of phenazine biosynthetic gene expression. PMID:24510163

Wide-field spectrally resolved quantitative fluorescence imaging system: toward neurosurgical guidance in glioma resection

NASA Astrophysics Data System (ADS)

Xie, Yijing; Thom, Maria; Ebner, Michael; Wykes, Victoria; Desjardins, Adrien; Miserocchi, Anna; Ourselin, Sebastien; McEvoy, Andrew W.; Vercauteren, Tom

2017-11-01

In high-grade glioma surgery, tumor resection is often guided by intraoperative fluorescence imaging. 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) provides fluorescent contrast between normal brain tissue and glioma tissue, thus achieving improved tumor delineation and prolonged patient survival compared with conventional white-light-guided resection. However, commercially available fluorescence imaging systems rely solely on visual assessment of fluorescence patterns by the surgeon, which makes the resection more subjective than necessary. We developed a wide-field spectrally resolved fluorescence imaging system utilizing a Generation II scientific CMOS camera and an improved computational model for the precise reconstruction of the PpIX concentration map. In our model, the tissue's optical properties and illumination geometry, which distort the fluorescent emission spectra, are considered. We demonstrate that the CMOS-based system can detect low PpIX concentration at short camera exposure times, while providing high-pixel resolution wide-field images. We show that total variation regularization improves the contrast-to-noise ratio of the reconstructed quantitative concentration map by approximately twofold. Quantitative comparison between the estimated PpIX concentration and tumor histopathology was also investigated to further evaluate the system.

Highly time-resolved imaging of combustion and pyrolysis product concentrations in solid fuel combustion: NO formation in a burning cigarette.

PubMed

Zimmermann, Ralf; Hertz-Schünemann, Romy; Ehlert, Sven; Liu, Chuan; McAdam, Kevin; Baker, Richard; Streibel, Thorsten

2015-02-03

The highly dynamic, heterogeneous combustion process within a burning cigarette was investigated by a miniaturized extractive sampling probe (microprobe) coupled to photoionization mass spectrometry using soft laser single photon ionization (SPI) for online real-time detection of molecular ions of combustion and pyrolysis products. Research cigarettes smoked by a smoking machine are used as a reproducible model system for solid-state biomass combustion, which up to now is not addressable by current combustion-diagnostic tools. By combining repetitively recorded online measurement sequences from different sampling locations in an imaging approach, highly time- and space-resolved quantitative distribution maps of, e.g., nitrogen monoxide, benzene, and oxygen concentrations were obtained at a near microscopic level. The obtained quantitative distribution maps represent a time-resolved, movie-like imaging of the respective compound's formation and destruction zones in the various combustion and pyrolysis regions of a cigarette during puffing. Furthermore, spatially resolved kinetic data were ascertainable. The here demonstrated methodology can also be applied to various heterogenic combustion/pyrolysis or reaction model systems, such as fossil- or biomass-fuel pellet combustion or to a positional resolved analysis of heterogenic catalytic reactions.

Retinal ganglion cell topography and spatial resolving power in penguins.

PubMed

Coimbra, João Paulo; Nolan, Paul M; Collin, Shaun P; Hart, Nathan S

2012-01-01

Penguins are a group of flightless seabirds that exhibit numerous morphological, behavioral and ecological adaptations to their amphibious lifestyle, but little is known about the topographic organization of neurons in their retinas. In this study, we used retinal wholemounts and stereological methods to estimate the total number and topographic distribution of retinal ganglion cells in addition to an anatomical estimate of spatial resolving power in two species of penguins: the little penguin, Eudyptula minor, and the king penguin, Aptenodytes patagonicus. The total number of ganglion cells per retina was approximately 1,200,000 in the little penguin and 1,110,000 in the king penguin. The topographic distribution of retinal ganglion cells in both species revealed the presence of a prominent horizontal visual streak with steeper gradients in the little penguin. The little penguin retinas showed ganglion cell density peaks of 21,867 cells/mm², affording spatial resolution in water of 17.07-17.46 cycles/degree (12.81-13.09 cycles/degree in air). In contrast, the king penguin showed a relatively lower peak density of ganglion cells of 14,222 cells/mm², but--due to its larger eye--slightly higher spatial resolution in water of 20.40 cycles/degree (15.30 cycles/degree in air). In addition, we mapped the distribution of giant ganglion cells in both penguin species using Nissl-stained wholemounts. In both species, topographic mapping of this cell type revealed the presence of an area gigantocellularis with a concentric organization of isodensity contours showing a peak in the far temporal retina of approximately 70 cells/mm² in the little penguin and 39 cells/mm² in the king penguin. Giant ganglion cell densities gradually fall towards the outermost isodensity contours revealing the presence of a vertically organized streak. In the little penguin, we confirmed our cytological characterization of giant ganglion cells using immunohistochemistry for microtubule

The Lyman alpha reference sample. VII. Spatially resolved Hα kinematics

NASA Astrophysics Data System (ADS)

Herenz, Edmund Christian; Gruyters, Pieter; Orlitova, Ivana; Hayes, Matthew; Östlin, Göran; Cannon, John M.; Roth, Martin M.; Bik, Arjan; Pardy, Stephen; Otí-Floranes, Héctor; Mas-Hesse, J. Miguel; Adamo, Angela; Atek, Hakim; Duval, Florent; Guaita, Lucia; Kunth, Daniel; Laursen, Peter; Melinder, Jens; Puschnig, Johannes; Rivera-Thorsen, Thøger E.; Schaerer, Daniel; Verhamme, Anne

2016-03-01

We present integral field spectroscopic observations with the Potsdam Multi-Aperture Spectrophotometer of all 14 galaxies in the z ~ 0.1 Lyman Alpha Reference Sample (LARS). We produce 2D line-of-sight velocity maps and velocity dispersion maps from the Balmer α (Hα) emission in our data cubes. These maps trace the spectral and spatial properties of the LARS galaxies' intrinsic Lyα radiation field. We show our kinematic maps that are spatially registered onto the Hubble Space Telescope Hα and Lyman α (Lyα) images. We can conjecture a causal connection between spatially resolved Hα kinematics and Lyα photometry for individual galaxies, however, no general trend can be established for the whole sample. Furthermore, we compute the intrinsic velocity dispersion σ0, the shearing velocity vshear, and the vshear/σ0 ratio from our kinematic maps. In general LARS galaxies are characterised by high intrinsic velocity dispersions (54 km s-1 median) and low shearing velocities (65 km s-1 median). The vshear/σ0 values range from 0.5 to 3.2 with an average of 1.5. It is noteworthy that five galaxies of the sample are dispersion-dominated systems with vshear/σ0< 1, and are thus kinematically similar to turbulent star-forming galaxies seen at high redshift. When linking our kinematical statistics to the global LARS Lyα properties, we find that dispersion-dominated systems show higher Lyα equivalent widths and higher Lyα escape fractions than systems with vshear/σ0> 1. Our result indicates that turbulence in actively star-forming systems is causally connected to interstellar medium conditions that favour an escape of Lyα radiation. Based on observations collected at the Centro Astronómico Hispano Alemán (CAHA) at Calar Alto, operated jointly by the Max-Planck Institut für Astronomie and the Instituto de Astrofísica de Andalucía (CSIC).The reduced data cubes (FITS files) are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130

Understanding the scatter in the spatially resolved star formation main sequence of local massive spiral galaxies

NASA Astrophysics Data System (ADS)

Abdurro'uf; Akiyama, Masayuki

2017-08-01

We investigate the relation between star formation rate (SFR) and stellar mass (M*) at the sub-galactic scale (˜1 kpc) of 93 local (0.01 < z < 0.02) massive (M* > 1010.5 M⊙) spiral galaxies. To derive a spatially resolved SFR and stellar mass, we perform the so-called pixel-to-pixel spectral energy distribution (SED) fitting, which fits an observed spatially resolved multiband SED with a library of model SEDs using Bayesian statistics. We use two bands (far-ultraviolet or FUV and near-ultraviolet or NUV) and five bands (u, g, r, I and z) of imaging data from Galaxy Evolution Explorer (GALEX) and Sloan Digital Sky Survey (SDSS), respectively. We find a tight nearly linear relation between the local surface density of SFR (ΣSFR) and stellar mass (Σ*), which is flattened at high Σ*. The near linear relation between Σ* and ΣSFR suggests a constant specific SFR (sSFR) throughout the galaxies, and the scatter of the relation is directly related to that of the sSFR. Therefore, we analyse the variation of the sSFR in various scales. More massive galaxies on average have lower sSFR throughout them than less massive galaxies. We also find that barred galaxies have a lower sSFR in the core region than non-barred galaxies. However, in the outer region, the sSFRs of barred and non-barred galaxies are similar and lead to a similar total sSFR.

Coupling of oceanic carbon and nitrogen facilitates spatially resolved quantitative reconstruction of nitrate inventories.

PubMed

Glock, Nicolaas; Erdem, Zeynep; Wallmann, Klaus; Somes, Christopher J; Liebetrau, Volker; Schönfeld, Joachim; Gorb, Stanislav; Eisenhauer, Anton

2018-03-23

Anthropogenic impacts are perturbing the global nitrogen cycle via warming effects and pollutant sources such as chemical fertilizers and burning of fossil fuels. Understanding controls on past nitrogen inventories might improve predictions for future global biogeochemical cycling. Here we show the quantitative reconstruction of deglacial bottom water nitrate concentrations from intermediate depths of the Peruvian upwelling region, using foraminiferal pore density. Deglacial nitrate concentrations correlate strongly with downcore δ 13 C, consistent with modern water column observations in the intermediate Pacific, facilitating the use of δ 13 C records as a paleo-nitrate-proxy at intermediate depths and suggesting that the carbon and nitrogen cycles were closely coupled throughout the last deglaciation in the Peruvian upwelling region. Combining the pore density and intermediate Pacific δ 13 C records shows an elevated nitrate inventory of >10% during the Last Glacial Maximum relative to the Holocene, consistent with a δ 13 C-based and δ 15 N-based 3D ocean biogeochemical model and previous box modeling studies.

Electronic transport characterization of silicon wafers by spatially resolved steady-state photocarrier radiometric imaging

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Qian; University of the Chinese Academy of Sciences, Beijing 100039; Li, Bincheng, E-mail: bcli@ioe.ac.cn

2015-09-28

Spatially resolved steady-state photocarrier radiometric (PCR) imaging technique is developed to characterize the electronic transport properties of silicon wafers. Based on a nonlinear PCR theory, simulations are performed to investigate the effects of electronic transport parameters (the carrier lifetime, the carrier diffusion coefficient, and the front surface recombination velocity) on the steady-state PCR intensity profiles. The electronic transport parameters of an n-type silicon wafer are simultaneously determined by fitting the measured steady-state PCR intensity profiles to the three-dimensional nonlinear PCR model. The determined transport parameters are in good agreement with the results obtained by the conventional modulated PCR technique withmore » multiple pump beam radii.« less

Spatially resolved multicolor CsPbX 3 nanowire heterojunctions via anion exchange

DOE PAGES

Dou, Letian; Lai, Minliang; Kley, Christopher S.; ...

2017-06-26

Halide perovskites are promising semiconductor materials for solution-processed optoelectronic devices. Their strong ionic bonding nature results in highly dynamic crystal lattices, inherently allowing rapid ion exchange at the solid–vapor and solid–liquid interface. In this paper, we show that the anion-exchange chemistry can be precisely controlled in single-crystalline halide perovskite nanomaterials when combined with nanofabrication techniques. We demonstrate spatially resolved multicolor CsPbX 3 (X = Cl, Br, I, or alloy of two halides) nanowire heterojunctions with a pixel size down to 500 nm with the photoluminescence tunable over the entire visible spectrum. In addition, the heterojunctions show distinct electronic states acrossmore » the interface, as revealed by Kelvin probe force microscopy. Finally, these perovskite heterojunctions represent key building blocks for high-resolution multicolor displays beyond current state-of-the-art technology as well as high-density diode/transistor arrays.« less

Spatially resolved X-ray emission measurements of the residual velocity during the stagnation phase of inertial confinement fusion implosion experiments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ruby, J. J.; Pak, A., E-mail: pak5@llnl.gov; Field, J. E.

2016-07-15

A technique for measuring residual motion during the stagnation phase of an indirectly driven inertial confinement experiment has been implemented. This method infers a velocity from spatially and temporally resolved images of the X-ray emission from two orthogonal lines of sight. This work investigates the accuracy of recovering spatially resolved velocities from the X-ray emission data. A detailed analytical and numerical modeling of the X-ray emission measurement shows that the accuracy of this method increases as the displacement that results from a residual velocity increase. For the typical experimental configuration, signal-to-noise ratios, and duration of X-ray emission, it is estimatedmore » that the fractional error in the inferred velocity rises above 50% as the velocity of emission falls below 24 μm/ns. By inputting measured parameters into this model, error estimates of the residual velocity as inferred from the X-ray emission measurements are now able to be generated for experimental data. Details of this analysis are presented for an implosion experiment conducted with an unintentional radiation flux asymmetry. The analysis shows a bright localized region of emission that moves through the larger emitting volume at a relatively higher velocity towards the location of the imposed flux deficit. This technique allows for the possibility of spatially resolving velocity flows within the so-called central hot spot of an implosion. This information would help to refine our interpretation of the thermal temperature inferred from the neutron time of flight detectors and the effect of localized hydrodynamic instabilities during the stagnation phase. Across several experiments, along a single line of sight, the average difference in magnitude and direction of the measured residual velocity as inferred from the X-ray and neutron time of flight detectors was found to be ∼13 μm/ns and ∼14°, respectively.« less

Spatially resolved X-ray emission measurements of the residual velocity during the stagnation phase of inertial confinement fusion implosion experiments

DOE PAGES

Ruby, J. J.; Pak, A.; Field, J. E.; ...

2016-07-01

A technique for measuring residual motion during the stagnation phase of an indirectly driven inertial confinement experiment has been implemented. Our method infers a velocity from spatially and temporally resolved images of the X-ray emission from two orthogonal lines of sight. This work investigates the accuracy of recovering spatially resolved velocities from the X-ray emission data. A detailed analytical and numerical modeling of the X-ray emission measurement shows that the accuracy of this method increases as the displacement that results from a residual velocity increase. For the typical experimental configuration, signal-to-noise ratios, and duration of X-ray emission, it is estimatedmore » that the fractional error in the inferred velocity rises above 50% as the velocity of emission falls below 24 μm/ns. Furthermore, by inputting measured parameters into this model, error estimates of the residual velocity as inferred from the X-ray emission measurements are now able to be generated for experimental data. Details of this analysis are presented for an implosion experiment conducted with an unintentional radiation flux asymmetry. The analysis shows a bright localized region of emission that moves through the larger emitting volume at a relatively higher velocity towards the location of the imposed flux deficit. Our technique allows for the possibility of spatially resolving velocity flows within the so-called central hot spot of an implosion. This information would help to refine our interpretation of the thermal temperature inferred from the neutron time of flight detectors and the effect of localized hydrodynamic instabilities during the stagnation phase. Across several experiments, along a single line of sight, the average difference in magnitude and direction of the measured residual velocity as inferred from the X-ray and neutron time of flight detectors was found to be ~13 μm/ns and ~14°, respectively.« less

Hyperspectral imaging-based spatially-resolved technique for accurate measurement of the optical properties of horticultural products

NASA Astrophysics Data System (ADS)

Cen, Haiyan

Hyperspectral imaging-based spatially-resolved technique is promising for determining the optical properties and quality attributes of horticultural and food products. However, considerable challenges still exist for accurate determination of spectral absorption and scattering properties from intact horticultural products. The objective of this research was, therefore, to develop and optimize hyperspectral imaging-based spatially-resolved technique for accurate measurement of the optical properties of horticultural products. Monte Carlo simulations and experiments for model samples of known optical properties were performed to optimize the inverse algorithm of a single-layer diffusion model and the optical designs, for extracting the absorption (micro a) and reduced scattering (micros') coefficients from spatially-resolved reflectance profiles. The logarithm and integral data transformation and the relative weighting methods were found to greatly improve the parameter estimation accuracy with the relative errors of 10.4%, 10.7%, and 11.4% for micro a, and 6.6%, 7.0%, and 7.1% for micros', respectively. More accurate measurements of optical properties were obtained when the light beam was of Gaussian type with the diameter of less than 1 mm, and the minimum and maximum source-detector distances were 1.5 mm and 10--20 transport mean free paths, respectively. An optical property measuring prototype was built, based on the optimization results, and evaluated for automatic measurement of absorption and reduced scattering coefficients for the wavelengths of 500--1,000 nm. The instrument was used to measure the optical properties, and assess quality/maturity, of 500 'Redstar' peaches and 1039 'Golden Delicious' (GD) and 1040 'Delicious' (RD) apples. A separate study was also conducted on confocal laser scanning and scanning electron microscopic image analysis and compression test of fruit tissue specimens to measure the structural and mechanical properties of 'Golden

Diagnosing the plasma nonuniformity in an iron opacity experiment by spatially resolved Al 1s-2p absorption spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang Xiaoding; Research Center of Laser Fusion, P. O. Box 919-986, Mianyang 621900; Zhang Jiyan

Generating a well-characterized hot-dense sample is of great importance to high quality opacity measurements. In this paper, we report on an experimental investigation of the plasma nonuniformity in a radiatively heated iron opacity sample by spatially resolved Al 1s-2p absorption spectroscopy. The iron sample was tamped by plastic at both sides and was heated by thermal x-ray radiation generated in a gold Hohlraum, and an Al layer attached to it was used as a tracer for temperature diagnosis. Spatially resolved 1s-2p transition absorption spectra of the Al tracer were measured by the technique of point-projection-spectroscopy, and temperatures in the samplemore » were obtained by comparing the measured spectra with detailed-term-accounting model calculations, with the density of the sample deduced using a combination of side-on radiography and radiative hydrodynamic simulation. The results showed the existence of axial temperature nonuniformity in the sample, and these temperature variations have been used to explain the shift of iron 2p-3d transition absorption feature along the axial direction of the Hohlraum used to heat the sample successfully.« less

An enhanced droplet-based liquid microjunction surface sampling system coupled with HPLC-ESI-MS/MS for spatially resolved analysis

DOE PAGES

Van Berkel, Gary J.; Weiskittel, Taylor M.; Kertesz, Vilmos

2014-11-07

Droplet-based liquid microjunction surface sampling coupled with high-performance liquid chromatography (HPLC)-electrospray ionization (ESI)-tandem mass spectrometry (MS/MS) for spatially resolved analysis provides the possibility of effective analysis of complex matrix samples and can provide a greater degree of chemical information from a single spot sample than is typically possible with a direct analysis of an extract. Described here is the setup and enhanced capabilities of a discrete droplet liquid microjunction surface sampling system employing a commercially available CTC PAL autosampler. The system enhancements include incorporation of a laser distance sensor enabling unattended analysis of samples and sample locations of dramatically disparatemore » height as well as reliably dispensing just 0.5 μL of extraction solvent to make the liquid junction to the surface, wherein the extraction spot size was confined to an area about 0.7 mm in diameter; software modifications improving the spatial resolution of sampling spot selection from 1.0 to 0.1 mm; use of an open bed tray system to accommodate samples as large as whole-body rat thin tissue sections; and custom sample/solvent holders that shorten sampling time to approximately 1 min per sample. Lastly, the merit of these new features was demonstrated by spatially resolved sampling, HPLC separation, and mass spectral detection of pharmaceuticals and metabolites from whole-body rat thin tissue sections and razor blade (“crude”) cut mouse tissue.« less

An enhanced droplet-based liquid microjunction surface sampling system coupled with HPLC-ESI-MS/MS for spatially resolved analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Van Berkel, Gary J.; Weiskittel, Taylor M.; Kertesz, Vilmos

Droplet-based liquid microjunction surface sampling coupled with high-performance liquid chromatography (HPLC)-electrospray ionization (ESI)-tandem mass spectrometry (MS/MS) for spatially resolved analysis provides the possibility of effective analysis of complex matrix samples and can provide a greater degree of chemical information from a single spot sample than is typically possible with a direct analysis of an extract. Described here is the setup and enhanced capabilities of a discrete droplet liquid microjunction surface sampling system employing a commercially available CTC PAL autosampler. The system enhancements include incorporation of a laser distance sensor enabling unattended analysis of samples and sample locations of dramatically disparatemore » height as well as reliably dispensing just 0.5 μL of extraction solvent to make the liquid junction to the surface, wherein the extraction spot size was confined to an area about 0.7 mm in diameter; software modifications improving the spatial resolution of sampling spot selection from 1.0 to 0.1 mm; use of an open bed tray system to accommodate samples as large as whole-body rat thin tissue sections; and custom sample/solvent holders that shorten sampling time to approximately 1 min per sample. Lastly, the merit of these new features was demonstrated by spatially resolved sampling, HPLC separation, and mass spectral detection of pharmaceuticals and metabolites from whole-body rat thin tissue sections and razor blade (“crude”) cut mouse tissue.« less

Spatially and time-resolved magnetization dynamics driven by spin-orbit torques

NASA Astrophysics Data System (ADS)

Baumgartner, Manuel; Garello, Kevin; Mendil, Johannes; Avci, Can Onur; Grimaldi, Eva; Murer, Christoph; Feng, Junxiao; Gabureac, Mihai; Stamm, Christian; Acremann, Yves; Finizio, Simone; Wintz, Sebastian; Raabe, Jörg; Gambardella, Pietro

2017-10-01

Current-induced spin-orbit torques are one of the most effective ways to manipulate the magnetization in spintronic devices, and hold promise for fast switching applications in non-volatile memory and logic units. Here, we report the direct observation of spin-orbit-torque-driven magnetization dynamics in Pt/Co/AlOx dots during current pulse injection. Time-resolved X-ray images with 25 nm spatial and 100 ps temporal resolution reveal that switching is achieved within the duration of a subnanosecond current pulse by the fast nucleation of an inverted domain at the edge of the dot and propagation of a tilted domain wall across the dot. The nucleation point is deterministic and alternates between the four dot quadrants depending on the sign of the magnetization, current and external field. Our measurements reveal how the magnetic symmetry is broken by the concerted action of the damping-like and field-like spin-orbit torques and the Dzyaloshinskii-Moriya interaction, and show that reproducible switching events can be obtained for over 1012 reversal cycles.

A quantitative method for determining spatial discriminative capacity.

PubMed

Zhang, Zheng; Tannan, Vinay; Holden, Jameson K; Dennis, Robert G; Tommerdahl, Mark

2008-03-10

The traditional two-point discrimination (TPD) test, a widely used tactile spatial acuity measure, has been criticized as being imprecise because it is based on subjective criteria and involves a number of non-spatial cues. The results of a recent study showed that as two stimuli were delivered simultaneously, vibrotactile amplitude discrimination became worse when the two stimuli were positioned relatively close together and was significantly degraded when the probes were within a subject's two-point limen. The impairment of amplitude discrimination with decreasing inter-probe distance suggested that the metric of amplitude discrimination could possibly provide a means of objective and quantitative measurement of spatial discrimination capacity. A two alternative forced-choice (2AFC) tracking procedure was used to assess a subject's ability to discriminate the amplitude difference between two stimuli positioned at near-adjacent skin sites. Two 25 Hz flutter stimuli, identical except for a constant difference in amplitude, were delivered simultaneously to the hand dorsum. The stimuli were initially spaced 30 mm apart, and the inter-stimulus distance was modified on a trial-by-trial basis based on the subject's performance of discriminating the stimulus with higher intensity. The experiment was repeated via sequential, rather than simultaneous, delivery of the same vibrotactile stimuli. Results obtained from this study showed that the performance of the amplitude discrimination task was significantly degraded when the stimuli were delivered simultaneously and were near a subject's two-point limen. In contrast, subjects were able to correctly discriminate between the amplitudes of the two stimuli when they were sequentially delivered at all inter-probe distances (including those within the two-point limen), and improved when an adapting stimulus was delivered prior to simultaneously delivered stimuli. Subjects' capacity to discriminate the amplitude difference

Non-diffusive ignition of a gaseous reactive mixture following time-resolved, spatially distributed energy deposition

NASA Astrophysics Data System (ADS)

Kassoy, D. R.

2014-01-01

Systematic asymptotic methods are applied to the compressible conservation and state equations for a reactive gas, including transport terms, to develop a rational thermomechanical formulation for the ignition of a chemical reaction following time-resolved, spatially distributed thermal energy addition from an external source into a finite volume of gas. A multi-parameter asymptotic analysis is developed for a wide range of energy deposition levels relative to the initial internal energy in the volume when the heating timescale is short compared to the characteristic acoustic timescale of the volume. Below a quantitatively defined threshold for energy addition, a nearly constant volume heating process occurs, with a small but finite internal gas expansion Mach number. Very little added thermal energy is converted to kinetic energy. The gas expelled from the boundary of the hot, high-pressure spot is the source of mechanical disturbances (acoustic and shock waves) that propagate away into the neighbouring unheated gas. When the energy addition reaches the threshold value, the heating process is fully compressible with a substantial internal gas expansion Mach number, the source of blast waves propagating into the unheated environmental gas. This case corresponds to an extremely large non-dimensional hot-spot temperature and pressure. If the former is sufficiently large, a high activation energy chemical reaction is initiated on the short heating timescale. This phenomenon is in contrast to that for more modest levels of energy addition, where a thermal explosion occurs only after the familiar extended ignition delay period for a classical high activation reaction. Transport effects, modulated by an asymptotically small Knudsen number, are shown to be negligible unless a local gradient in temperature, concentration or velocity is exceptionally large.

A Perspective on the Application of Spatially Resolved ARPES for 2D Materials

PubMed Central

Cattelan, Mattia

2018-01-01

In this paper, a perspective on the application of Spatially- and Angle-Resolved PhotoEmission Spectroscopy (ARPES) for the study of two-dimensional (2D) materials is presented. ARPES allows the direct measurement of the electronic band structure of materials generating extremely useful insights into their electronic properties. The possibility to apply this technique to 2D materials is of paramount importance because these ultrathin layers are considered fundamental for future electronic, photonic and spintronic devices. In this review an overview of the technical aspects of spatially localized ARPES is given along with a description of the most advanced setups for laboratory and synchrotron-based equipment. This technique is sensitive to the lateral dimensions of the sample. Therefore, a discussion on the preparation methods of 2D material is presented. Some of the most interesting results obtained by ARPES are reported in three sections including: graphene, transition metal dichalcogenides (TMDCs) and 2D heterostructures. Graphene has played a key role in ARPES studies because it inspired the use of this technique with other 2D materials. TMDCs are presented for their peculiar transport, optical and spin properties. Finally, the section featuring heterostructures highlights a future direction for research into 2D material structures. PMID:29702567

A Perspective on the Application of Spatially Resolved ARPES for 2D Materials.

PubMed

Cattelan, Mattia; Fox, Neil A

2018-04-27

In this paper, a perspective on the application of Spatially- and Angle-Resolved PhotoEmission Spectroscopy (ARPES) for the study of two-dimensional (2D) materials is presented. ARPES allows the direct measurement of the electronic band structure of materials generating extremely useful insights into their electronic properties. The possibility to apply this technique to 2D materials is of paramount importance because these ultrathin layers are considered fundamental for future electronic, photonic and spintronic devices. In this review an overview of the technical aspects of spatially localized ARPES is given along with a description of the most advanced setups for laboratory and synchrotron-based equipment. This technique is sensitive to the lateral dimensions of the sample. Therefore, a discussion on the preparation methods of 2D material is presented. Some of the most interesting results obtained by ARPES are reported in three sections including: graphene, transition metal dichalcogenides (TMDCs) and 2D heterostructures. Graphene has played a key role in ARPES studies because it inspired the use of this technique with other 2D materials. TMDCs are presented for their peculiar transport, optical and spin properties. Finally, the section featuring heterostructures highlights a future direction for research into 2D material structures.

In situ distributed diagnostics of flowable electrode systems: resolving spatial and temporal limitations.

PubMed

Dennison, C R; Gogotsi, Y; Kumbur, E C

2014-09-14

In this study, we have developed an in situ distributed diagnostics tool to investigate spatial and temporal effects in electrochemical systems based on flowable electrodes. Specifically, an experimental approach was developed that enables spatially-resolved voltage measurements to be obtained in situ, in real-time. To extract additional data from these distributed measurements, an experimentally-parameterized equivalent circuit model with a new 'flow capacitor' circuit element was developed to predict the distributions of various system parameters during operation. As a case study, this approach was applied to investigate the behavior of the suspension electrodes used in an electrochemical flow capacitor under flowing and static conditions. The volumetric capacitance is reduced from 15.6 F ml(-1) to 1.1 F ml(-1) under flowing conditions. Results indicate that the majority of the charging in suspension electrodes occurs within ∼750 μm of the current collectors during flow, which gives rise to significant state-of-charge gradients across the cell, as well as underutilization of the available active material. The underlying cause of this observation is attributed to the relatively high electrical resistance of the slurry coupled with a stratified charging regime and insufficient residence time. The observations highlight the need to develop more conductive slurries and to design cells with reduced charge transport lengths.

The spatially resolved star formation history of CALIFA galaxies. Cosmic time scales

NASA Astrophysics Data System (ADS)

García-Benito, R.; González Delgado, R. M.; Pérez, E.; Cid Fernandes, R.; Cortijo-Ferrero, C.; López Fernández, R.; de Amorim, A. L.; Lacerda, E. A. D.; Vale Asari, N.; Sánchez, S. F.

2017-12-01

This paper presents the mass assembly time scales of nearby galaxies observed by CALIFA at the 3.5 m telescope in Calar Alto. We apply the fossil record method of the stellar populations to the complete sample of the 3rd CALIFA data release, with a total of 661 galaxies, covering stellar masses from 108.4 to 1012M⊙ and a wide range of Hubble types. We apply spectral synthesis techniques to the datacubes and process the results to produce the mass growth time scales and mass weighted ages, from which we obtain temporal and spatially resolved information in seven bins of galaxy morphology (E, S0, Sa, Sb, Sc, and Sd) and six bins of stellar mass and stellar mass surface density. We use three different tracers of the spatially resolved star formation history (mass assembly curves, ratio of half mass to half light radii, and mass-weighted age gradients) to test if galaxies grow inside-out, and its dependence with galaxy stellar mass, stellar mass surface density, and morphology. Our main results are as follows: (a) the innermost regions of galaxies assemble their mass at an earlier time than regions located in the outer parts; this happens at any given stellar mass (M⋆), stellar mass surface density (Σ⋆), or Hubble type, including the lowest mass systems in our sample. (b) Galaxies present a significant diversity in their characteristic formation epochs for lower-mass systems. This diversity shows a strong dependence of the mass assembly time scales on Σ⋆ and Hubble type in the lower-mass range (108.4 to 1010.4), but a very mild dependence in higher-mass bins. (c) The lowest half mass radius (HMR) to half light radius (HLR) ratio is found for galaxies between 1010.4 and 1011.1M⊙, where galaxies are 25% smaller in mass than in light. Low-mass galaxies show the largest ratio with HMR/HLR 0.89. Sb and Sbc galaxies present the lowest HMR/HLR ratio (0.74). The ratio HMR/HLR is always, on average, below 1, indicating that galaxies grow faster in mass than in light

Spatially resolved measurement of singlet delta oxygen by radar resonance-enhanced multiphoton ionization.

PubMed

Wu, Yue; Zhang, Zhili; Ombrello, Timothy M

2013-07-01

Coherent microwave Rayleigh scattering (Radar) from resonance-enhanced multiphoton ionization (REMPI) was demonstrated to directly and nonintrusively measure singlet delta oxygen, O(2)(a(1)Δ(g)), with high spatial resolution. Two different approaches, photodissociation of ozone and microwave discharge plasma in an argon and oxygen flow, were utilized for O(2)(a(1)Δ(g)) generation. The d(1)Π(g)←a(1)Δ(g) (3-0) and d(1)Π(g)←a(1)Δ(g) (1-0) bands of O(2)(a(1)Δ(g)) were detected by Radar REMPI for two different flow conditions. Quantitative absorption measurements using sensitive off-axis integrated cavity output spectroscopy (ICOS) was used simultaneously to evaluate the accuracy and sensitivity of the Radar REMPI technique. The detection limit of Radar REMPI was found to be comparable to the ICOS technique with a detection threshold of approximately 10(14) molecules/cm(3) but with a spatial resolution that was 8 orders of magnitude smaller than the ICOS technique.

Real-time and quantitative isotropic spatial resolution susceptibility imaging for magnetic nanoparticles

NASA Astrophysics Data System (ADS)

Pi, Shiqiang; Liu, Wenzhong; Jiang, Tao

2018-03-01

The magnetic transparency of biological tissue allows the magnetic nanoparticle (MNP) to be a promising functional sensor and contrast agent. The complex susceptibility of MNPs, strongly influenced by particle concentration, excitation magnetic field and their surrounding microenvironment, provides significant implications for biomedical applications. Therefore, magnetic susceptibility imaging of high spatial resolution will give more detailed information during the process of MNP-aided diagnosis and therapy. In this study, we present a novel spatial magnetic susceptibility extraction method for MNPs under a gradient magnetic field, a low-frequency drive magnetic field, and a weak strength high-frequency magnetic field. Based on this novel method, a magnetic particle susceptibility imaging (MPSI) of millimeter-level spatial resolution (<3 mm) was achieved using our homemade imaging system. Corroborated by the experimental results, the MPSI shows real-time (1 s per frame acquisition) and quantitative abilities, and isotropic high resolution.

SDSS IV MaNGA: Dependence of Global and Spatially Resolved SFR–M ∗ Relations on Galaxy Properties

NASA Astrophysics Data System (ADS)

Pan, Hsi-An; Lin, Lihwai; Hsieh, Bau-Ching; Sánchez, Sebastián F.; Ibarra-Medel, Héctor; Boquien, Médéric; Lacerna, Ivan; Argudo-Fernández, Maria; Bizyaev, Dmitry; Cano-Díaz, Mariana; Drory, Niv; Gao, Yang; Masters, Karen; Pan, Kaike; Tabor, Martha; Tissera, Patricia; Xiao, Ting

2018-02-01

The galaxy integrated Hα star formation rate–stellar mass relation, or SFR(global)–M *(global) relation, is crucial for understanding star formation history and evolution of galaxies. However, many studies have dealt with SFR using unresolved measurements, which makes it difficult to separate out the contamination from other ionizing sources, such as active galactic nuclei and evolved stars. Using the integral field spectroscopic observations from SDSS-IV MaNGA, we spatially disentangle the contribution from different Hα powering sources for ∼1000 galaxies. We find that, when including regions dominated by all ionizing sources in galaxies, the spatially resolved relation between Hα surface density (ΣHα (all)) and stellar mass surface density (Σ*(all)) progressively turns over at the high Σ*(all) end for increasing M *(global) and/or bulge dominance (bulge-to-total light ratio, B/T). This in turn leads to the flattening of the integrated Hα(global)–M *(global) relation in the literature. By contrast, there is no noticeable flattening in both integrated Hα(H II)–M *(H II) and spatially resolved ΣHα (H II)–Σ*(H II) relations when only regions where star formation dominates the ionization are considered. In other words, the flattening can be attributed to the increasing regions powered by non-star-formation sources, which generally have lower ionizing ability than star formation. An analysis of the fractional contribution of non-star-formation sources to total Hα luminosity of a galaxy suggests a decreasing role of star formation as an ionizing source toward high-mass, high-B/T galaxies and bulge regions. This result indicates that the appearance of the galaxy integrated SFR–M * relation critically depends on their global properties (M *(global) and B/T) and relative abundances of various ionizing sources within the galaxies.

Investigation of microstructure in additive manufactured Inconel 625 by spatially resolved neutron transmission spectroscopy

DOE PAGES

Tremsin, Anton S.; Gao, Yan; Dial, Laura C.; ...

2016-07-08

Non-destructive testing techniques based on neutron imaging and diffraction can provide information on the internal structure of relatively thick metal samples (up to several cm), which are opaque to other conventional non-destructive methods. Spatially resolved neutron transmission spectroscopy is an extension of traditional neutron radiography, where multiple images are acquired simultaneously, each corresponding to a narrow range of energy. The analysis of transmission spectra enables studies of bulk microstructures at the spatial resolution comparable to the detector pixel. In this study we demonstrate the possibility of imaging (with ~100 μm resolution) distribution of some microstructure properties, such as residual strain,more » texture, voids and impurities in Inconel 625 samples manufactured with an additive manufacturing method called direct metal laser melting (DMLM). Although this imaging technique can be implemented only in a few large-scale facilities, it can be a valuable tool for optimization of additive manufacturing techniques and materials and for correlating bulk microstructure properties to manufacturing process parameters. Additionally, the experimental strain distribution can help validate finite element models which many industries use to predict the residual stress distributions in additive manufactured components.« less

Investigation of microstructure in additive manufactured Inconel 625 by spatially resolved neutron transmission spectroscopy.

PubMed

Tremsin, Anton S; Gao, Yan; Dial, Laura C; Grazzi, Francesco; Shinohara, Takenao

2016-01-01

Non-destructive testing techniques based on neutron imaging and diffraction can provide information on the internal structure of relatively thick metal samples (up to several cm), which are opaque to other conventional non-destructive methods. Spatially resolved neutron transmission spectroscopy is an extension of traditional neutron radiography, where multiple images are acquired simultaneously, each corresponding to a narrow range of energy. The analysis of transmission spectra enables studies of bulk microstructures at the spatial resolution comparable to the detector pixel. In this study we demonstrate the possibility of imaging (with ~100 μm resolution) distribution of some microstructure properties, such as residual strain, texture, voids and impurities in Inconel 625 samples manufactured with an additive manufacturing method called direct metal laser melting (DMLM). Although this imaging technique can be implemented only in a few large-scale facilities, it can be a valuable tool for optimization of additive manufacturing techniques and materials and for correlating bulk microstructure properties to manufacturing process parameters. In addition, the experimental strain distribution can help validate finite element models which many industries use to predict the residual stress distributions in additive manufactured components.

Investigation of microstructure in additive manufactured Inconel 625 by spatially resolved neutron transmission spectroscopy

NASA Astrophysics Data System (ADS)

Tremsin, Anton S.; Gao, Yan; Dial, Laura C.; Grazzi, Francesco; Shinohara, Takenao

2016-01-01

Non-destructive testing techniques based on neutron imaging and diffraction can provide information on the internal structure of relatively thick metal samples (up to several cm), which are opaque to other conventional non-destructive methods. Spatially resolved neutron transmission spectroscopy is an extension of traditional neutron radiography, where multiple images are acquired simultaneously, each corresponding to a narrow range of energy. The analysis of transmission spectra enables studies of bulk microstructures at the spatial resolution comparable to the detector pixel. In this study we demonstrate the possibility of imaging (with 100 μm resolution) distribution of some microstructure properties, such as residual strain, texture, voids and impurities in Inconel 625 samples manufactured with an additive manufacturing method called direct metal laser melting (DMLM). Although this imaging technique can be implemented only in a few large-scale facilities, it can be a valuable tool for optimization of additive manufacturing techniques and materials and for correlating bulk microstructure properties to manufacturing process parameters. In addition, the experimental strain distribution can help validate finite element models which many industries use to predict the residual stress distributions in additive manufactured components.

Investigation of microstructure in additive manufactured Inconel 625 by spatially resolved neutron transmission spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tremsin, Anton S.; Gao, Yan; Dial, Laura C.

Non-destructive testing techniques based on neutron imaging and diffraction can provide information on the internal structure of relatively thick metal samples (up to several cm), which are opaque to other conventional non-destructive methods. Spatially resolved neutron transmission spectroscopy is an extension of traditional neutron radiography, where multiple images are acquired simultaneously, each corresponding to a narrow range of energy. The analysis of transmission spectra enables studies of bulk microstructures at the spatial resolution comparable to the detector pixel. In this study we demonstrate the possibility of imaging (with ~100 μm resolution) distribution of some microstructure properties, such as residual strain,more » texture, voids and impurities in Inconel 625 samples manufactured with an additive manufacturing method called direct metal laser melting (DMLM). Although this imaging technique can be implemented only in a few large-scale facilities, it can be a valuable tool for optimization of additive manufacturing techniques and materials and for correlating bulk microstructure properties to manufacturing process parameters. Additionally, the experimental strain distribution can help validate finite element models which many industries use to predict the residual stress distributions in additive manufactured components.« less

Investigation of microstructure in additive manufactured Inconel 625 by spatially resolved neutron transmission spectroscopy

PubMed Central

Tremsin, Anton S.; Gao, Yan; Dial, Laura C.; Grazzi, Francesco; Shinohara, Takenao

2016-01-01

Abstract Non-destructive testing techniques based on neutron imaging and diffraction can provide information on the internal structure of relatively thick metal samples (up to several cm), which are opaque to other conventional non-destructive methods. Spatially resolved neutron transmission spectroscopy is an extension of traditional neutron radiography, where multiple images are acquired simultaneously, each corresponding to a narrow range of energy. The analysis of transmission spectra enables studies of bulk microstructures at the spatial resolution comparable to the detector pixel. In this study we demonstrate the possibility of imaging (with ~100 μm resolution) distribution of some microstructure properties, such as residual strain, texture, voids and impurities in Inconel 625 samples manufactured with an additive manufacturing method called direct metal laser melting (DMLM). Although this imaging technique can be implemented only in a few large-scale facilities, it can be a valuable tool for optimization of additive manufacturing techniques and materials and for correlating bulk microstructure properties to manufacturing process parameters. In addition, the experimental strain distribution can help validate finite element models which many industries use to predict the residual stress distributions in additive manufactured components. PMID:27877885

Spatially resolved air-water emissions tradeoffs improve regulatory impact analyses for electricity generation.

PubMed

Gingerich, Daniel B; Sun, Xiaodi; Behrer, A Patrick; Azevedo, Inês L; Mauter, Meagan S

2017-02-21

Coal-fired power plants (CFPPs) generate air, water, and solids emissions that impose substantial human health, environmental, and climate change (HEC) damages. This work demonstrates the importance of accounting for cross-media emissions tradeoffs, plant and regional emissions factors, and spatially variation in the marginal damages of air emissions when performing regulatory impact analyses for electric power generation. As a case study, we assess the benefits and costs of treating wet flue gas desulfurization (FGD) wastewater at US CFPPs using the two best available treatment technology options specified in the 2015 Effluent Limitation Guidelines (ELGs). We perform a life-cycle inventory of electricity and chemical inputs to FGD wastewater treatment processes and quantify the marginal HEC damages of associated air emissions. We combine these spatially resolved damage estimates with Environmental Protection Agency estimates of water quality benefits, fuel-switching benefits, and regulatory compliance costs. We estimate that the ELGs will impose average net costs of $3.01 per cubic meter for chemical precipitation and biological wastewater treatment and $11.26 per cubic meter for zero-liquid discharge wastewater treatment (expected cost-benefit ratios of 1.8 and 1.7, respectively), with damages concentrated in regions containing a high fraction of coal generation or a large chemical manufacturing industry. Findings of net cost for FGD wastewater treatment are robust to uncertainty in auxiliary power source, location of chemical manufacturing, and binding air emissions limits in noncompliant regions, among other variables. Future regulatory design will minimize compliance costs and HEC tradeoffs by regulating air, water, and solids emissions simultaneously and performing regulatory assessments that account for spatial variation in emissions impacts.

Spatially resolved air-water emissions tradeoffs improve regulatory impact analyses for electricity generation

PubMed Central

Gingerich, Daniel B.; Behrer, A. Patrick; Azevedo, Inês L.

2017-01-01

Coal-fired power plants (CFPPs) generate air, water, and solids emissions that impose substantial human health, environmental, and climate change (HEC) damages. This work demonstrates the importance of accounting for cross-media emissions tradeoffs, plant and regional emissions factors, and spatially variation in the marginal damages of air emissions when performing regulatory impact analyses for electric power generation. As a case study, we assess the benefits and costs of treating wet flue gas desulfurization (FGD) wastewater at US CFPPs using the two best available treatment technology options specified in the 2015 Effluent Limitation Guidelines (ELGs). We perform a life-cycle inventory of electricity and chemical inputs to FGD wastewater treatment processes and quantify the marginal HEC damages of associated air emissions. We combine these spatially resolved damage estimates with Environmental Protection Agency estimates of water quality benefits, fuel-switching benefits, and regulatory compliance costs. We estimate that the ELGs will impose average net costs of $3.01 per cubic meter for chemical precipitation and biological wastewater treatment and $11.26 per cubic meter for zero-liquid discharge wastewater treatment (expected cost-benefit ratios of 1.8 and 1.7, respectively), with damages concentrated in regions containing a high fraction of coal generation or a large chemical manufacturing industry. Findings of net cost for FGD wastewater treatment are robust to uncertainty in auxiliary power source, location of chemical manufacturing, and binding air emissions limits in noncompliant regions, among other variables. Future regulatory design will minimize compliance costs and HEC tradeoffs by regulating air, water, and solids emissions simultaneously and performing regulatory assessments that account for spatial variation in emissions impacts. PMID:28167772

Assessing temporally and spatially resolved PM 2.5 exposures for epidemiological studies using satellite aerosol optical depth measurements

NASA Astrophysics Data System (ADS)

Kloog, Itai; Koutrakis, Petros; Coull, Brent A.; Lee, Hyung Joo; Schwartz, Joel

2011-11-01

Land use regression (LUR) models provide good estimates of spatially resolved long-term exposures, but are poor at capturing short term exposures. Satellite-derived Aerosol Optical Depth (AOD) measurements have the potential to provide spatio-temporally resolved predictions of both long and short term exposures, but previous studies have generally showed relatively low predictive power. Our objective was to extend our previous work on day-specific calibrations of AOD data using ground PM 2.5 measurements by incorporating commonly used LUR variables and meteorological variables, thus benefiting from both the spatial resolution from the LUR models and the spatio-temporal resolution from the satellite models. Later we use spatial smoothing to predict PM 2.5 concentrations for day/locations with missing AOD measures. We used mixed models with random slopes for day to calibrate AOD data for 2000-2008 across New-England with monitored PM 2.5 measurements. We then used a generalized additive mixed model with spatial smoothing to estimate PM 2.5 in location-day pairs with missing AOD, using regional measured PM 2.5, AOD values in neighboring cells, and land use. Finally, local (100 m) land use terms were used to model the difference between grid cell prediction and monitored value to capture very local traffic particles. Out-of-sample ten-fold cross-validation was used to quantify the accuracy of our predictions. For days with available AOD data we found high out-of-sample R2 (mean out-of-sample R2 = 0.830, year to year variation 0.725-0.904). For days without AOD values, our model performance was also excellent (mean out-of-sample R2 = 0.810, year to year variation 0.692-0.887). Importantly, these R2 are for daily, rather than monthly or yearly, values. Our model allows one to assess short term and long-term human exposures in order to investigate both the acute and chronic effects of ambient particles, respectively.

A Hybrid Model for Spatially and Temporally Resolved Ozone Exposures in the Continental United States

PubMed Central

Di, Qian; Rowland, Sebastian; Koutrakis, Petros; Schwartz, Joel

2017-01-01

Ground-level ozone is an important atmospheric oxidant, which exhibits considerable spatial and temporal variability in its concentration level. Existing modeling approaches for ground-level ozone include chemical transport models, land-use regression, Kriging, and data fusion of chemical transport models with monitoring data. Each of these methods has both strengths and weaknesses. Combining those complementary approaches could improve model performance. Meanwhile, satellite-based total column ozone, combined with ozone vertical profile, is another potential input. We propose a hybrid model that integrates the above variables to achieve spatially and temporally resolved exposure assessments for ground-level ozone. We used a neural network for its capacity to model interactions and nonlinearity. Convolutional layers, which use convolution kernels to aggregate nearby information, were added to the neural network to account for spatial and temporal autocorrelation. We trained the model with AQS 8-hour daily maximum ozone in the continental United States from 2000 to 2012 and tested it with left out monitoring sites. Cross-validated R2 on the left out monitoring sites ranged from 0.74 to 0.80 (mean 0.76) for predictions on 1 km×1 km grid cells, which indicates good model performance. Model performance remains good even at low ozone concentrations. The prediction results facilitate epidemiological studies to assess the health effect of ozone in the long term and the short term. PMID:27332675

A comparative analysis of two highly spatially resolved European atmospheric emission inventories

NASA Astrophysics Data System (ADS)

Ferreira, J.; Guevara, M.; Baldasano, J. M.; Tchepel, O.; Schaap, M.; Miranda, A. I.; Borrego, C.

2013-08-01

A reliable emissions inventory is highly important for air quality modelling applications, especially at regional or local scales, which require high resolutions. Consequently, higher resolution emission inventories have been developed that are suitable for regional air quality modelling. This research performs an inter-comparative analysis of different spatial disaggregation methodologies of atmospheric emission inventories. This study is based on two different European emission inventories with different spatial resolutions: 1) the EMEP (European Monitoring and Evaluation Programme) inventory and 2) an emission inventory developed by the TNO (Netherlands Organisation for Applied Scientific Research). These two emission inventories were converted into three distinct gridded emission datasets as follows: (i) the EMEP emission inventory was disaggregated by area (EMEParea) and (ii) following a more complex methodology (HERMES-DIS - High-Elective Resolution Modelling Emissions System - DISaggregation module) to understand and evaluate the influence of different disaggregation methods; and (iii) the TNO gridded emissions, which are based on different emission data sources and different disaggregation methods. A predefined common grid with a spatial resolution of 12 × 12 km2 was used to compare the three datasets spatially. The inter-comparative analysis was performed by source sector (SNAP - Selected Nomenclature for Air Pollution) with emission totals for selected pollutants. It included the computation of difference maps (to focus on the spatial variability of emission differences) and a linear regression analysis to calculate the coefficients of determination and to quantitatively measure differences. From the spatial analysis, greater differences were found for residential/commercial combustion (SNAP02), solvent use (SNAP06) and road transport (SNAP07). These findings were related to the different spatial disaggregation that was conducted by the TNO and HERMES

Effects of finite spatial resolution on quantitative CBF images from dynamic PET

DOE Office of Scientific and Technical Information (OSTI.GOV)

Phelps, M.E.; Huang, S.C.; Mahoney, D.K.

1985-05-01

The finite spatial resolution of PET causes the time-activity responses on pixels around the boundaries between gray and white matter regions to contain kinetic components from tissues of different CBF's. CBF values estimated from kinetics of such mixtures are underestimated because of the nonlinear relationship between the time-activity response and the estimated CBF. Computer simulation is used to investigate these effects on phantoms of circular structures and realistic brain slice in terms of object size and quantitative CBF values. The CBF image calculated is compared to the case of having resolution loss alone. Results show that the size of amore » high flow region in the CBF image is decreased while that of a low flow region is increased. For brain phantoms, the qualitative appearance of CBF images is not seriously affected, but the estimated CBF's are underestimated by 11 to 16 percent in local gray matter regions (of size 1 cm/sup 2/) with about 14 percent reduction in global CBF over the whole slice. It is concluded that the combined effect of finite spatial resolution and the nonlinearity in estimating CBF from dynamic PET is quite significant and must be considered in processing and interpreting quantitative CBF images.« less

An Integrative Platform for Three-dimensional Quantitative Analysis of Spatially Heterogeneous Metastasis Landscapes

NASA Astrophysics Data System (ADS)

Guldner, Ian H.; Yang, Lin; Cowdrick, Kyle R.; Wang, Qingfei; Alvarez Barrios, Wendy V.; Zellmer, Victoria R.; Zhang, Yizhe; Host, Misha; Liu, Fang; Chen, Danny Z.; Zhang, Siyuan

2016-04-01

Metastatic microenvironments are spatially and compositionally heterogeneous. This seemingly stochastic heterogeneity provides researchers great challenges in elucidating factors that determine metastatic outgrowth. Herein, we develop and implement an integrative platform that will enable researchers to obtain novel insights from intricate metastatic landscapes. Our two-segment platform begins with whole tissue clearing, staining, and imaging to globally delineate metastatic landscape heterogeneity with spatial and molecular resolution. The second segment of our platform applies our custom-developed SMART 3D (Spatial filtering-based background removal and Multi-chAnnel forest classifiers-based 3D ReconsTruction), a multi-faceted image analysis pipeline, permitting quantitative interrogation of functional implications of heterogeneous metastatic landscape constituents, from subcellular features to multicellular structures, within our large three-dimensional (3D) image datasets. Coupling whole tissue imaging of brain metastasis animal models with SMART 3D, we demonstrate the capability of our integrative pipeline to reveal and quantify volumetric and spatial aspects of brain metastasis landscapes, including diverse tumor morphology, heterogeneous proliferative indices, metastasis-associated astrogliosis, and vasculature spatial distribution. Collectively, our study demonstrates the utility of our novel integrative platform to reveal and quantify the global spatial and volumetric characteristics of the 3D metastatic landscape with unparalleled accuracy, opening new opportunities for unbiased investigation of novel biological phenomena in situ.

A 64-pixel NbTiN superconducting nanowire single-photon detector array for spatially resolved photon detection.

PubMed

Miki, Shigehito; Yamashita, Taro; Wang, Zhen; Terai, Hirotaka

2014-04-07

We present the characterization of two-dimensionally arranged 64-pixel NbTiN superconducting nanowire single-photon detector (SSPD) array for spatially resolved photon detection. NbTiN films deposited on thermally oxidized Si substrates enabled the high-yield production of high-quality SSPD pixels, and all 64 SSPD pixels showed uniform superconducting characteristics within the small range of 7.19-7.23 K of superconducting transition temperature and 15.8-17.8 μA of superconducting switching current. Furthermore, all of the pixels showed single-photon sensitivity, and 60 of the 64 pixels showed a pulse generation probability higher than 90% after photon absorption. As a result of light irradiation from the single-mode optical fiber at different distances between the fiber tip and the active area, the variations of system detection efficiency (SDE) in each pixel showed reasonable Gaussian distribution to represent the spatial distributions of photon flux intensity.

Spatially-resolved mapping of history-dependent coupled electrochemical and electronical behaviors of electroresistive NiO

DOE PAGES

Sugiyama, Issei; Kim, Yunseok; Jesse, Stephen; ...

2014-10-22

Bias-induced oxygen ion dynamics underpins a broad spectrum of electroresistive and memristive phenomena in oxide materials. Although widely studied by device-level and local voltage-current spectroscopies, the relationship between electroresistive phenomena, local electrochemical behaviors, and microstructures remains elusive. Here, the interplay between history-dependent electronic transport and electrochemical phenomena in a NiO single crystalline thin film with a number of well-defined defect types is explored on the nanometer scale using an atomic force microscopy-based technique. A variety of electrochemically-active regions were observed and spatially resolved relationship between the electronic and electrochemical phenomena was revealed. The regions with pronounced electroresistive activity were furthermore » correlated with defects identified by scanning transmission electron microscopy. Using fully coupled mechanical-electrochemical modeling, we illustrate that the spatial distribution of strain plays an important role in electrochemical and electroresistive phenomena. In conclusion, these studies illustrate an approach for simultaneous mapping of the electronic and ionic transport on a single defective structure level such as dislocations or interfaces, and pave the way for creating libraries of defect-specific electrochemical responses.« less

SPATIALLY RESOLVED HCN J = 4-3 AND CS J = 7-6 EMISSION FROM THE DISK AROUND HD 142527

DOE Office of Scientific and Technical Information (OSTI.GOV)

Van der Plas, G.; Casassus, S.; Perez, S.

2014-09-10

The disk around HD 142527 attracts a great amount of attention compared to others because of its resolved (sub-)millimeter dust continuum that is concentrated into the shape of a horseshoe toward the north of the star. In this Letter we present spatially resolved ALMA detections of the HCN J = 4-3 and CS J = 7-6 emission lines. These lines give us a deeper view into the disk compared to the (optically thicker) CO isotopes. This is the first detection of CS J = 7-6 coming from a protoplanetary disk. Both emission lines are azimuthally asymmetric and are suppressed under the horseshoe-shapedmore » continuum emission peak. A possible mechanism for explaining the decrease under the horseshoe-shaped continuum is the increased opacity coming from the higher dust concentration at the continuum peak. Lower dust and/or gas temperatures and an optically thick radio-continuum reduce line emission by freezing out and shielding emission from the far side of the disk.« less

Assessment and Mission Planning Capability For Quantitative Aerothermodynamic Flight Measurements Using Remote Imaging

NASA Technical Reports Server (NTRS)

Horvath, Thomas; Splinter, Scott; Daryabeigi, Kamran; Wood, William; Schwartz, Richard; Ross, Martin

2008-01-01

High resolution calibrated infrared imagery of vehicles during hypervelocity atmospheric entry or sustained hypersonic cruise has the potential to provide flight data on the distribution of surface temperature and the state of the airflow over the vehicle. In the early 1980 s NASA sought to obtain high spatial resolution infrared imagery of the Shuttle during entry. Despite mission execution with a technically rigorous pre-planning capability, the single airborne optical system for this attempt was considered developmental and the scientific return was marginal. In 2005 the Space Shuttle Program again sponsored an effort to obtain imagery of the Orbiter. Imaging requirements were targeted towards Shuttle ascent; companion requirements for entry did not exist. The engineering community was allowed to define observation goals and incrementally demonstrate key elements of a quantitative spatially resolved measurement capability over a series of flights. These imaging opportunities were extremely beneficial and clearly demonstrated capability to capture infrared imagery with mature and operational assets of the US Navy and the Missile Defense Agency. While successful, the usefulness of the imagery was, from an engineering perspective, limited. These limitations were mainly associated with uncertainties regarding operational aspects of data acquisition. These uncertainties, in turn, came about because of limited pre-flight mission planning capability, a poor understanding of several factors including the infrared signature of the Shuttle, optical hardware limitations, atmospheric effects and detector response characteristics. Operational details of sensor configuration such as detector integration time and tracking system algorithms were carried out ad hoc (best practices) which led to low probability of target acquisition and detector saturation. Leveraging from the qualified success during Return-to-Flight, the NASA Engineering and Safety Center sponsored an

Fast spatially resolved exhaust gas recirculation (EGR) distribution measurements in an internal combustion engine using absorption spectroscopy.

PubMed

Yoo, Jihyung; Prikhodko, Vitaly; Parks, James E; Perfetto, Anthony; Geckler, Sam; Partridge, William P

2015-09-01

Exhaust gas recirculation (EGR) in internal combustion engines is an effective method of reducing NOx emissions while improving efficiency. However, insufficient mixing between fresh air and exhaust gas can lead to cycle-to-cycle and cylinder-to-cylinder non-uniform charge gas mixtures of a multi-cylinder engine, which can in turn reduce engine performance and efficiency. A sensor packaged into a compact probe was designed, built and applied to measure spatiotemporal EGR distributions in the intake manifold of an operating engine. The probe promotes the development of more efficient and higher-performance engines by resolving high-speed in situ CO2 concentration at various locations in the intake manifold. The study employed mid-infrared light sources tuned to an absorption band of CO2 near 4.3 μm, an industry standard species for determining EGR fraction. The calibrated probe was used to map spatial EGR distributions in an intake manifold with high accuracy and monitor cycle-resolved cylinder-specific EGR fluctuations at a rate of up to 1 kHz.

Fast Spatially Resolved Exhaust Gas Recirculation (EGR) Distribution Measurements in an Internal Combustion Engine Using Absorption Spectroscopy

DOE PAGES

Yoo, Jihyung; Prikhodko, Vitaly; Parks, James E.; ...

2015-09-01

One effective method of reducing NO x emissions while improving efficiency is exhaust gas recirculation (EGR) in internal combustion engines. But, insufficient mixing between fresh air and exhaust gas can lead to cycle-to-cycle and cylinder-to-cylinder nonuniform charge gas mixtures of a multi-cylinder engine, which can in turn reduce engine performance and efficiency. Furthermore, a sensor packaged into a compact probe was designed, built and applied to measure spatiotemporal EGR distributions in the intake manifold of an operating engine. The probe promotes the development of more efficient and higher-performance engines by resolving high-speed in situ CO 2 concentration at various locationsmore » in the intake manifold. Our study employed mid-infrared light sources tuned to an absorption band of CO 2 near 4.3 μm, an industry standard species for determining EGR fraction. The calibrated probe was used to map spatial EGR distributions in an intake manifold with high accuracy and monitor cycle-resolved cylinder-specific EGR fluctuations at a rate of up to 1 kHz.« less

Measurements of spatially resolved velocity variations in shock compressed heterogeneous materials using a line-imaging velocity interferometer

NASA Astrophysics Data System (ADS)

Trott, Wayne M.; Knudson, Marcus D.; Chhabildas, Lalit C.; Asay, James R.

2000-04-01

Relatively straightforward changes in the design of a conventional optically recording velocity interferometer system (ORVIS) can be used to produce a line-imaging instrument that allows adjustment of spatial resolution over a wide range. As a result, line-imaging ORVIS can be tailored to various specific applications involving dynamic deformation of heterogeneous materials as required by their characteristic length scales (ranging from a few μm for ferroelectric ceramics to a few mm for concrete). A line-imaging system has been successfully interfaced to a compressed gas gun driver and fielded on numerous tests in combination with simultaneous dual delay-leg, "push-pull" VISAR measurements. These tests include shock loading of glass-reinforced polyester composites, foam reverberation experiments (measurements at the free surface of a thin aluminum plate impacted by foam), and measurements of dispersive velocity in a shock-loaded explosive simulant (sugar). Results are presented that illustrate the capability for recording detailed spatially resolved material response.

A spatially resolving x-ray crystal spectrometer for measurement of ion-temperature and rotation-velocity profiles on the Alcator C-Mod tokamak.

PubMed

Hill, K W; Bitter, M L; Scott, S D; Ince-Cushman, A; Reinke, M; Rice, J E; Beiersdorfer, P; Gu, M-F; Lee, S G; Broennimann, Ch; Eikenberry, E F

2008-10-01

A new spatially resolving x-ray crystal spectrometer capable of measuring continuous spatial profiles of high resolution spectra (lambda/d lambda>6000) of He-like and H-like Ar K alpha lines with good spatial (approximately 1 cm) and temporal (approximately 10 ms) resolutions has been installed on the Alcator C-Mod tokamak. Two spherically bent crystals image the spectra onto four two-dimensional Pilatus II pixel detectors. Tomographic inversion enables inference of local line emissivity, ion temperature (T(i)), and toroidal plasma rotation velocity (upsilon(phi)) from the line Doppler widths and shifts. The data analysis techniques, T(i) and upsilon(phi) profiles, analysis of fusion-neutron background, and predictions of performance on other tokamaks, including ITER, will be presented.

Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders.

PubMed

Marquet, Pierre; Depeursinge, Christian; Magistretti, Pierre J

2014-10-01

Quantitative phase microscopy (QPM) has recently emerged as a new powerful quantitative imaging technique well suited to noninvasively explore a transparent specimen with a nanometric axial sensitivity. In this review, we expose the recent developments of quantitative phase-digital holographic microscopy (QP-DHM). Quantitative phase-digital holographic microscopy (QP-DHM) represents an important and efficient quantitative phase method to explore cell structure and dynamics. In a second part, the most relevant QPM applications in the field of cell biology are summarized. A particular emphasis is placed on the original biological information, which can be derived from the quantitative phase signal. In a third part, recent applications obtained, with QP-DHM in the field of cellular neuroscience, namely the possibility to optically resolve neuronal network activity and spine dynamics, are presented. Furthermore, potential applications of QPM related to psychiatry through the identification of new and original cell biomarkers that, when combined with a range of other biomarkers, could significantly contribute to the determination of high risk developmental trajectories for psychiatric disorders, are discussed.

Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders

PubMed Central

Marquet, Pierre; Depeursinge, Christian; Magistretti, Pierre J.

2014-01-01

Abstract. Quantitative phase microscopy (QPM) has recently emerged as a new powerful quantitative imaging technique well suited to noninvasively explore a transparent specimen with a nanometric axial sensitivity. In this review, we expose the recent developments of quantitative phase-digital holographic microscopy (QP-DHM). Quantitative phase-digital holographic microscopy (QP-DHM) represents an important and efficient quantitative phase method to explore cell structure and dynamics. In a second part, the most relevant QPM applications in the field of cell biology are summarized. A particular emphasis is placed on the original biological information, which can be derived from the quantitative phase signal. In a third part, recent applications obtained, with QP-DHM in the field of cellular neuroscience, namely the possibility to optically resolve neuronal network activity and spine dynamics, are presented. Furthermore, potential applications of QPM related to psychiatry through the identification of new and original cell biomarkers that, when combined with a range of other biomarkers, could significantly contribute to the determination of high risk developmental trajectories for psychiatric disorders, are discussed. PMID:26157976

Pilot Assessment of Brain Metabolism in Perinatally HIV-Infected Youths Using Accelerated 5D Echo Planar J-Resolved Spectroscopic Imaging.

PubMed

Iqbal, Zohaib; Wilson, Neil E; Keller, Margaret A; Michalik, David E; Church, Joseph A; Nielsen-Saines, Karin; Deville, Jaime; Souza, Raissa; Brecht, Mary-Lynn; Thomas, M Albert

2016-01-01

To measure cerebral metabolite levels in perinatally HIV-infected youths and healthy controls using the accelerated five dimensional (5D) echo planar J-resolved spectroscopic imaging (EP-JRESI) sequence, which is capable of obtaining two dimensional (2D) J-resolved spectra from three spatial dimensions (3D). After acquisition and reconstruction of the 5D EP-JRESI data, T1-weighted MRIs were used to classify brain regions of interest for HIV patients and healthy controls: right frontal white (FW), medial frontal gray (FG), right basal ganglia (BG), right occipital white (OW), and medial occipital gray (OG). From these locations, respective J-resolved and TE-averaged spectra were extracted and fit using two different quantitation methods. The J-resolved spectra were fit using prior knowledge fitting (ProFit) while the TE-averaged spectra were fit using the advanced method for accurate robust and efficient spectral fitting (AMARES). Quantitation of the 5D EP-JRESI data using the ProFit algorithm yielded significant metabolic differences in two spatial locations of the perinatally HIV-infected youths compared to controls: elevated NAA/(Cr+Ch) in the FW and elevated Asp/(Cr+Ch) in the BG. Using the TE-averaged data quantified by AMARES, an increase of Glu/(Cr+Ch) was shown in the FW region. A strong negative correlation (r < -0.6) was shown between tCh/(Cr+Ch) quantified using ProFit in the FW and CD4 counts. Also, strong positive correlations (r > 0.6) were shown between Asp/(Cr+Ch) and CD4 counts in the FG and BG. The complimentary results using ProFit fitting of J-resolved spectra and AMARES fitting of TE-averaged spectra, which are a subset of the 5D EP-JRESI acquisition, demonstrate an abnormal energy metabolism in the brains of perinatally HIV-infected youths. This may be a result of the HIV pathology and long-term combinational anti-retroviral therapy (cART). Further studies of larger perinatally HIV-infected cohorts are necessary to confirm these findings.

The spatially resolved star formation history of mergers. A comparative study of the LIRGs IC 1623, NGC 6090, NGC 2623, and Mice

NASA Astrophysics Data System (ADS)

Cortijo-Ferrero, C.; González Delgado, R. M.; Pérez, E.; Cid Fernandes, R.; García-Benito, R.; Di Matteo, P.; Sánchez, S. F.; de Amorim, A. L.; Lacerda, E. A. D.; López Fernández, R.; Tadhunter, C.

2017-11-01

This paper presents the spatially resolved star formation history (2D-SFH) of a small sample of four local mergers: the early-stage mergers IC 1623, NGC 6090, and the Mice, and the more advanced merger NGC 2623, by analyzing IFS data from the CALIFA survey and PMAS in LArr mode. Full spectral fitting techniques are applied to the datacubes to obtain the spatially resolved mass growth histories, the time evolution of the star formation rate intensity (ΣSFR), and the local specific star formation rate (sSFR), over three different time scales (30 Myr, 300 Myr, and 1 Gyr). The results are compared with non-interacting Sbc-Sc galaxies, to quantify if there is an enhancement of the star formation and to trace its time scale and spatial extent. Our results for the three LIRGs (IC 1623 W, NGC 6090, and NGC 2623) show that a major phase of star formation is occurring in time scales of 107 yr to few 108 yr, with global SFR enhancements of between approximately two and six with respect to main-sequence star forming (MSSF) galaxies. In the two early-stage mergers IC 1623 W and NGC 6090, which are between first pericentre passage and coalescence, the most remarkable increase of the SFR with respect to non-interacting spirals occurred in the last 30 Myr, and it is spatially extended, with enhancements of factors between two and seven both in the centres (r < 0.5 half light radius, HLR), and in the disks (r > 1 HLR). In the more advanced merger NGC 2623 an extended phase of star formation occurred on a longer time scale of 1 Gyr, with a SFR enhancement of a factor of approximately two-to-three larger than the one in Sbc-Sc MSSF galaxies over the same period, probably relic of the first pericentre passage epoch. A SFR enhancement in the last 30 Myr is also present, but only in NGC 2623 centre, by a factor of three. In general, the spatially resolved SFHs of the LIRG-mergers are consistent with the predictions from high spatial resolution simulations. In contrast, the star

A double-label time-resolved fluorescent strip for rapidly quantitative detection of carbofuran residues in agro-products.

PubMed

Zhang, Qi; Qu, Qiaoyu; Chen, Shanshan; Liu, Xiaowei; Li, Peiwu

2017-09-15

A rapid and quantitative time-resolved fluorescent immunochromatographic assay (TRFICA) for detecting carbofuran residues in agro-products was reported in this paper. This assay was developed based on double-label immunoprobes, one of which was a carbofuran-specific antibody coupled with europium microbeads for the test (T) line signal while the other was mouse IgG coupled with europium microbeads for the control (C) line signal. Quantitative relationships between carbofuran concentrations and T/C ratios were established to determine the analyte concentration. To increase assay accuracy, four standard curves were established for the agro-products (green bean, cabbage, apple, and pear). The limits of detection (LODs) ranged from 0.04 to 0.76mgL -1 . The spiked recoveries of carbofuran in the agro-products were in the range of 81-103%, which was in good agreement with a standard HPLC method. Therefore, we provided a new and reliable method for determination of N-methylcarbamate pesticide carbofuran residues in agro-products including vegetables and fruits. Copyright © 2017. Published by Elsevier Ltd.

Tunable Reflective Spatial Heterodyne Spectrometer: A Technique for High Resolving Power, Wide Field Of View Observation Of Diffuse Emission Line Sources

NASA Astrophysics Data System (ADS)

Hosseini, Seyedeh Sona

The purpose of this dissertation is to discuss the need for new technology in broadband high-resolution spectroscopy based on the emerging technique of Spatial Heterodyne Spectroscopy (SHS) and to propose new solutions that should enhance and generalize this technology to other fields. Spectroscopy is a proven tool for determining compositional and other properties of remote objects. Narrow band imaging and low resolving spectroscopic measurements provide information about composition, photochemical evolution, energy distribution and density. The extension to high resolving power provides further access to temperature, velocity, isotopic ratios, separation of blended sources, and opacity effects. In current high resolving power devices, the drawback of high-resolution spectroscopy is bound to the instrumental limitations of lower throughput, the necessity of small entrance apertures, sensitivity, field of view, and large physical instrumental size. These limitations quickly become handicapping for observation of faint and/or extended targets and for spacecraft encounters. A technique with promise for the study of faint and extended sources at high resolving power is the reflective format of the Spatial Heterodyne Spectrometer (SHS). SHS instruments are compact and naturally tailored for both high etendue (defined in section 2.2.5) and high resolving power. In contrast, to achieve similar spectral grasp, grating spectrometers require large telescopes. For reference, SHS is a cyclical interferometer that produces Fizeau fringe pattern for all other wavelengths except the tuned wavelength. The large etendue obtained by SHS instruments makes them ideal for observations of extended, low surface brightness, isolated emission line sources, while their intrinsically high spectral resolution enables one to study the dynamical and physical properties described above. This document contains four chapters. Chapter 1, introduces a class of scientific targets that formerly have

CO(J = 3-2) on-the-fly mapping of the nearby spiral galaxies NGC 628 and NGC 7793: Spatially resolved CO(J = 3-2) star-formation law

NASA Astrophysics Data System (ADS)

Muraoka, Kazuyuki; Takeda, Miho; Yanagitani, Kazuki; Kaneko, Hiroyuki; Nakanishi, Kouichiro; Kuno, Nario; Sorai, Kazuo; Tosaki, Tomoka; Kohno, Kotaro

2016-04-01

We present the results of CO(J = 3-2) on-the-fly mappings of two nearby non-barred spiral galaxies, NGC 628 and NGC 7793, with the Atacama Submillimeter Telescope Experiment at an effective angular resolution of 25″. We successfully obtained global distributions of CO(J = 3-2) emission over the entire disks at a sub-kpc resolution for both galaxies. We examined the spatially resolved (sub-kpc) relationship between CO(J = 3-2) luminosities (L^' }_CO(3-2)) and infrared (IR) luminosities (LIR) for NGC 628, NGC 7793, and M 83, and compared it with global luminosities of a JCMT (James Clerk Maxwell Telescope) Nearby Galaxy Legacy Survey sample. We found a striking linear L^' }_CO(3-2)-LIR correlation over the four orders of magnitude, and the correlation is consistent even with that for ultraluminous IR galaxies and submillimeter-selected galaxies. In addition, we examined the spatially resolved relationship between CO(J = 3-2) intensities (ICO(3-2)) and extinction-corrected star formation rates (SFRs) for NGC 628, NGC 7793, and M 83, and compared it with that for Giant Molecular Clouds in M 33 and 14 nearby galaxy centers. We found a linear ICO(3-2)-SFR correlation with ˜1 dex scatter. We conclude that the CO(J = 3-2) star-formation law (i.e., linear L^' }_CO(3-2)-LIR and ICO(3-2)-SFR correlations) is universally applicable to various types and spatial scales of galaxies; from spatially resolved nearby galaxy disks to distant IR-luminous galaxies, within ˜1 dex scatter.

Highly spatially- and seasonally-resolved predictive contamination maps for persistent organic pollutants: development and validation.

PubMed

Ballabio, Cristiano; Guazzoni, Niccoló; Comolli, Roberto; Tremolada, Paolo

2013-08-01

A reliable spatial assessment of the POPs contamination in soils is essential for burden studies and flux evaluations. Soil characteristics and properties vary enormously even within small spatial scale and over time; therefore soil capacity of accumulating POPs varies greatly. In order to include this very high spatial and temporal variability, models can be used for assessing soil accumulation capacity in a specific time and space and, from it, the spatial distribution and temporal trends of POPs concentrations. In this work, predictive contamination maps of the accumulation capacity of soils were developed at a space resolution of 1×1m with a time frame of one day, in a study area located in the central Alps. Physical algorithms for temperature and organic carbon estimation along the soil profile and across the year were fitted to estimate the horizontal, vertical and seasonal distribution of the contamination potential for PCBs in soil (Ksa maps). The resulting maps were cross-validated with an independent set of PCB contamination data, showing very good agreement (e.g. for CB-153, R(2)=0.80, p-value≤2.2·10(-06)). Slopes of the regression between predicted Ksa and experimental concentrations were used to map the soil contamination for the whole area, taking into account soil characteristics and temperature conditions. These maps offer the opportunity to evaluate burden (concentration maps) and fluxes (emission maps) with highly resolved temporal and spatial detail. In addition, in order to explain the observed low autumn PCB concentrations in soil related to the high Ksa values of this period, a dynamic model of seasonal variation of soil concentrations was developed basing on rate parameters fitted on measured concentrations. The model was able to describe, at least partially, the observed different behavior between the quite rapid discharge phase in summer and the slow recharge phase in autumn. Copyright © 2013 Elsevier B.V. All rights reserved.

Spatial bedrock erosion distribution in a natural gorge

NASA Astrophysics Data System (ADS)

Beer, A. R.; Turowski, J. M.; Kirchner, J. W.

2015-12-01

Quantitative analysis of morphological evolution both in terrestrial and planetary landscapes is of increasing interest in the geosciences. In mountainous regions, bedrock channel formation as a consequence of the interaction of uplift and erosion processes is fundamental for the entire surface evolution. Hence, the accurate description of bedrock channel development is important for landscape modelling. To verify existing concepts developed in the lab and to analyse how in situ channel erosion rates depend on the interrelations of discharge, sediment transport and topography, there is a need of highly resolved topographic field data. We analyse bedrock erosion over two years in a bedrock gorge downstream of the Gorner glacier above the town of Zermatt, Switzerland. At the study site, the Gornera stream cuts through a roche moutonnée in serpentine rock of 25m length, 5m width and 8m depth. We surveyed bedrock erosion rates using repeat terrestrial laser scanning (TLS) with an average point spacing of 5mm. Bedrock erosion rates in direction of the individual surface normals were studied directly on the scanned point clouds applying the M3C2 algorithm (Lague et al., 2013, ISPRS). The surveyed erosion patterns were compared to a simple stream erosivity visualisation obtained from painted bedrock sections at the study location. Spatially distributed erosion rates on bedrock surfaces based on millions of scan points allow deduction of millimeter-scale mean annual values of lateral erosion, incision and downstream erosion on protruding streambed surfaces. The erosion rate on a specific surface point is shown to depend on the position of this surface point in the channel's cross section, its height above the streambed and its spatial orientation to the streamflow. Abrasion by impacting bedload was likely the spatially dominant erosion process, as confirmed by the observed patterns along the painted bedrock sections. However, a single plucking event accounted for the half

Automated high resolution full-field spatial coherence tomography for quantitative phase imaging of human red blood cells

NASA Astrophysics Data System (ADS)

Singla, Neeru; Dubey, Kavita; Srivastava, Vishal; Ahmad, Azeem; Mehta, D. S.

2018-02-01

We developed an automated high-resolution full-field spatial coherence tomography (FF-SCT) microscope for quantitative phase imaging that is based on the spatial, rather than the temporal, coherence gating. The Red and Green color laser light was used for finding the quantitative phase images of unstained human red blood cells (RBCs). This study uses morphological parameters of unstained RBCs phase images to distinguish between normal and infected cells. We recorded the single interferogram by a FF-SCT microscope for red and green color wavelength and average the two phase images to further reduced the noise artifacts. In order to characterize anemia infected from normal cells different morphological features were extracted and these features were used to train machine learning ensemble model to classify RBCs with high accuracy.

SPATIALLY RESOLVED SPECTROSCOPY OF EUROPA: THE DISTINCT SPECTRUM OF LARGE-SCALE CHAOS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fischer, P. D.; Brown, M. E.; Hand, K. P., E-mail: pfischer@caltech.edu

2015-11-15

We present a comprehensive analysis of spatially resolved moderate spectral resolution near-infrared spectra obtained with the adaptive optics system at the Keck Observatory. We identify three compositionally distinct end member regions: the trailing hemisphere bullseye, the leading hemisphere upper latitudes, and a third component associated with leading hemisphere chaos units. We interpret the composition of the three end member regions to be dominated by irradiation products, water ice, and evaporite deposits or salt brines, respectively. The third component is associated with geological features and distinct from the geography of irradiation, suggesting an endogenous identity. Identifying the endogenous composition is ofmore » particular interest for revealing the subsurface composition. However, its spectrum is not consistent with linear mixtures of the salt minerals previously considered relevant to Europa. The spectrum of this component is distinguished by distorted hydration features rather than distinct spectral features, indicating hydrated minerals but making unique identification difficult. In particular, it lacks features common to hydrated sulfate minerals, challenging the traditional view of an endogenous salty component dominated by Mg-sulfates. Chloride evaporite deposits are one possible alternative.« less

Spatially Resolved HCN Absorption Features in the Circumnuclear Region of NGC 1052

NASA Astrophysics Data System (ADS)

Sawada-Satoh, Satoko; Roh, Duk-Gyoo; Oh, Se-Jin; Lee, Sang-Sung; Byun, Do-Young; Kameno, Seiji; Yeom, Jae-Hwan; Jung, Dong-Kyu; Kim, Hyo-Ryoung; Hwang, Ju-Yeon

2016-10-01

We present the first VLBI detection of HCN molecular absorption in the nearby active galactic nucleus NGC 1052. Utilizing the 1 mas resolution achieved by the Korean VLBI Network, we have spatially resolved the HCN absorption against a double-sided nuclear jet structure. Two velocity features of HCN absorption are detected significantly at the radial velocity of 1656 and 1719 km s-1, redshifted by 149 and 212 km s-1 with respect to the systemic velocity of the galaxy. The column density of the HCN molecule is estimated to be 1015-1016 cm-2, assuming an excitation temperature of 100-230 K. The absorption features show high optical depth localized on the receding jet side, where the free-free absorption occurred due to the circumnuclear torus. The size of the foreground absorbing molecular gas is estimated to be on approximately one-parsec scales, which agrees well with the approximate size of the circumnuclear torus. HCN absorbing gas is likely to be several clumps smaller than 0.1 pc inside the circumnuclear torus. The redshifted velocities of the HCN absorption features imply that HCN absorbing gas traces ongoing infall motion inside the circumnuclear torus onto the central engine.

Spatially-resolved mean flow and turbulence help explain observed erosion and deposition patterns of snow over Antarctic sea ice

NASA Astrophysics Data System (ADS)

Trujillo, E.; Giometto, M. G.; Leonard, K. C.; Maksym, T. L.; Meneveau, C. V.; Parlange, M. B.; Lehning, M.

2014-12-01

Sea ice-atmosphere interactions are major drivers of patterns of sea ice drift and deformations in the Polar regions, and affect snow erosion and deposition at the surface. Here, we combine analyses of sea ice surface topography at very high-resolutions (1-10 cm), and Large Eddy Simulations (LES) to study surface drag and snow erosion and deposition patterns from process scales to floe scales (1 cm - 100 m). The snow/ice elevations were obtained using a Terrestrial Laser Scanner during the SIPEX II (Sea Ice Physics and Ecosystem eXperiment II) research voyage to East Antarctica (September-November 2012). LES are performed on a regular domain adopting a mixed pseudo-spectral/finite difference spatial discretization. A scale-dependent dynamic subgrid-scale model based on Lagrangian time averaging is adopted to determine the eddy-viscosity in the bulk of the flow. Effects of larger-scale features of the surface on wind flows (those features that can be resolved in the LES) are accounted for through an immersed boundary method. Conversely, drag forces caused by subgrid-scale features of the surface should be accounted for through a parameterization. However, the effective aerodynamic roughness parameter z0 for snow/ice is not known. Hence, a novel dynamic approach is utilized, in which z0 is determined using the constraint that the total momentum flux (drag) must be independent on grid-filter scale. We focus on three ice floe surfaces. The first of these surfaces (October 6, 2012) is used to test the performance of the model, validate the algorithm, and study the spatial distributed fields of resolved and modeled stress components. The following two surfaces, scanned at the same location before and after a snow storm event (October 20/23, 2012), are used to propose an application to study how spatially resolved mean flow and turbulence relates to observed patterns of snow erosion and deposition. We show how erosion and deposition patterns are correlated with the

Real-time absorption and scattering characterization of slab-shaped turbid samples obtained by a combination of angular and spatially resolved measurements.

PubMed

Dam, Jan S; Yavari, Nazila; Sørensen, Søren; Andersson-Engels, Stefan

2005-07-10

We present a fast and accurate method for real-time determination of the absorption coefficient, the scattering coefficient, and the anisotropy factor of thin turbid samples by using simple continuous-wave noncoherent light sources. The three optical properties are extracted from recordings of angularly resolved transmittance in addition to spatially resolved diffuse reflectance and transmittance. The applied multivariate calibration and prediction techniques are based on multiple polynomial regression in combination with a Newton--Raphson algorithm. The numerical test results based on Monte Carlo simulations showed mean prediction errors of approximately 0.5% for all three optical properties within ranges typical for biological media. Preliminary experimental results are also presented yielding errors of approximately 5%. Thus the presented methods show a substantial potential for simultaneous absorption and scattering characterization of turbid media.

Event Centroiding Applied to Energy-Resolved Neutron Imaging at LANSCE

DOE PAGES

Borges, Nicholas; Losko, Adrian; Vogel, Sven

2018-02-13

The energy-dependence of the neutron cross section provides vastly different contrast mechanisms than polychromatic neutron radiography if neutron energies can be selected for imaging applications. In recent years, energy-resolved neutron imaging (ERNI) with epi-thermal neutrons, utilizing neutron absorption resonances for contrast as well as for quantitative density measurements, was pioneered at the Flight Path 5 beam line at LANSCE and continues to be refined. In this work, we present event centroiding, i.e., the determination of the center-of-gravity of a detection event on an imaging detector to allow sub-pixel spatial resolution and apply it to the many frames collected for energy-resolvedmore » neutron imaging at a pulsed neutron source. While event centroiding was demonstrated at thermal neutron sources, it has not been applied to energy-resolved neutron imaging, where the energy resolution requires to be preserved, and we present a quantification of the possible resolution as a function of neutron energy. For the 55 μm pixel size of the detector used for this study, we found a resolution improvement from ~80 μm to ~22 μm using pixel centroiding while fully preserving the energy resolution.« less

Event Centroiding Applied to Energy-Resolved Neutron Imaging at LANSCE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Borges, Nicholas; Losko, Adrian; Vogel, Sven

The energy-dependence of the neutron cross section provides vastly different contrast mechanisms than polychromatic neutron radiography if neutron energies can be selected for imaging applications. In recent years, energy-resolved neutron imaging (ERNI) with epi-thermal neutrons, utilizing neutron absorption resonances for contrast as well as for quantitative density measurements, was pioneered at the Flight Path 5 beam line at LANSCE and continues to be refined. In this work, we present event centroiding, i.e., the determination of the center-of-gravity of a detection event on an imaging detector to allow sub-pixel spatial resolution and apply it to the many frames collected for energy-resolvedmore » neutron imaging at a pulsed neutron source. While event centroiding was demonstrated at thermal neutron sources, it has not been applied to energy-resolved neutron imaging, where the energy resolution requires to be preserved, and we present a quantification of the possible resolution as a function of neutron energy. For the 55 μm pixel size of the detector used for this study, we found a resolution improvement from ~80 μm to ~22 μm using pixel centroiding while fully preserving the energy resolution.« less

Space- and time-resolved raman and breakdown spectroscopy: advanced lidar techniques

NASA Astrophysics Data System (ADS)

Silviu, Gurlui; Marius Mihai, Cazacu; Adrian, Timofte; Oana, Rusu; Georgiana, Bulai; Dimitriu, Dan

2018-04-01

DARLIOES - the advanced LIDAR is based on space- and time-resolved RAMAN and breakdown spectroscopy, to investigate chemical and toxic compounds, their kinetics and physical properties at high temporal (2 ns) and spatial (1 cm) resolution. The high spatial and temporal resolution are needed to resolve a large variety of chemical troposphere compounds, emissions from aircraft, the self-organization space charges induced light phenomena, temperature and humidity profiles, ice nucleation, etc.

Resolving runaway electron distributions in space, time, and energy

DOE PAGES

Paz-Soldan, Carlos; Cooper, C. M.; Aleynikov, P.; ...

2018-05-01

Areas of agreement and disagreement with present-day models of RE evolution are revealed by measuring MeV-level bremsstrahlung radiation from runaway electrons (REs) with a pinhole camera. Spatially-resolved measurements localize the RE beam, reveal energy-dependent RE transport, and can be used to perform full two-dimensional (energy and pitch-angle) inversions of the RE phase space distribution. Energy-resolved measurements find qualitative agreement with modeling on the role of collisional and synchrotron damping in modifying the RE distribution shape. Measurements are consistent with predictions of phase-space attractors that accumulate REs, with non-monotonic features observed in the distribution. Temporally-resolved measurements find qualitative agreement with modelingmore » on the impact of collisional and synchrotron damping in varying the RE growth and decay rate. Anomalous RE loss is observed and found to be largest at low energy. As a result, possible roles for kinetic instability or spatial transport to resolve these anomalies are discussed.« less

Resolving runaway electron distributions in space, time, and energy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Paz-Soldan, Carlos; Cooper, C. M.; Aleynikov, P.

Areas of agreement and disagreement with present-day models of RE evolution are revealed by measuring MeV-level bremsstrahlung radiation from runaway electrons (REs) with a pinhole camera. Spatially-resolved measurements localize the RE beam, reveal energy-dependent RE transport, and can be used to perform full two-dimensional (energy and pitch-angle) inversions of the RE phase space distribution. Energy-resolved measurements find qualitative agreement with modeling on the role of collisional and synchrotron damping in modifying the RE distribution shape. Measurements are consistent with predictions of phase-space attractors that accumulate REs, with non-monotonic features observed in the distribution. Temporally-resolved measurements find qualitative agreement with modelingmore » on the impact of collisional and synchrotron damping in varying the RE growth and decay rate. Anomalous RE loss is observed and found to be largest at low energy. As a result, possible roles for kinetic instability or spatial transport to resolve these anomalies are discussed.« less

Resolving runaway electron distributions in space, time, and energy

NASA Astrophysics Data System (ADS)

Paz-Soldan, C.; Cooper, C. M.; Aleynikov, P.; Eidietis, N. W.; Lvovskiy, A.; Pace, D. C.; Brennan, D. P.; Hollmann, E. M.; Liu, C.; Moyer, R. A.; Shiraki, D.

2018-05-01

Areas of agreement and disagreement with present-day models of runaway electron (RE) evolution are revealed by measuring MeV-level bremsstrahlung radiation from runaway electrons (REs) with a pinhole camera. Spatially resolved measurements localize the RE beam, reveal energy-dependent RE transport, and can be used to perform full two-dimensional (energy and pitch-angle) inversions of the RE phase-space distribution. Energy-resolved measurements find qualitative agreement with modeling on the role of collisional and synchrotron damping in modifying the RE distribution shape. Measurements are consistent with predictions of phase-space attractors that accumulate REs, with non-monotonic features observed in the distribution. Temporally resolved measurements find qualitative agreement with modeling on the impact of collisional and synchrotron damping in varying the RE growth and decay rate. Anomalous RE loss is observed and found to be largest at low energy. Possible roles for kinetic instability or spatial transport to resolve these anomalies are discussed.

A Quantitative Corpus-Based Approach to English Spatial Particles: Conceptual Symmetry and Its Pedagogical Implications

ERIC Educational Resources Information Center

Chen, Alvin Cheng-Hsien

2014-01-01

The present study aims to investigate how conceptual symmetry plays a role in the use of spatial particles in English and to further examine its pedagogical implications via a corpus-based evaluation of the course books in senior high schools in Taiwan. More specifically, we adopt a quantitative corpus-based approach to investigate whether bipolar…

Spatially Resolved Hard X-ray Emission in the Central 5 kpc of the Galaxy Merger NGC 6240

NASA Astrophysics Data System (ADS)

Wang, Junfeng; Nardini, E.; Fabbiano, G.; Karovska, M.; Elvis, M.; Pellegrini, S.; Max, C. E.; Risaliti, G.; U, V.; Zezas, A.

2013-04-01

We have obtained a deep, sub-arcsecond resolution X-ray image of the nuclear region of the luminous galaxy merger NGC 6240 with Chandra, which resolves the X-ray emission from the pair of active nuclei and the diffuse hot gas in great detail. We detect extended hard X-ray emission from 70 million K hot gas over a spatial scale of 5 kpc, indicating the presence of fast shocks with velocity of 2200 km/s. For the first time we obtain spatial distribution of this highly ionized gas emitting FeXXV and find that it shows a remarkable correspondence to the large scale morphology of H_2(1-0) S(1) line emission and Hα filaments. Propagation of fast shocks originated in the starburst driven wind into the ambient dense gas can account for this morphological correspondence. Both the energetics and the iron mass in the hot gas are consistent with the expected injection from the supernovae explosion during the starburst that is commensurate with its high star formation rate.

Label-free imaging of intracellular motility by low-coherent quantitative phase microscope in reflection geometry

NASA Astrophysics Data System (ADS)

Yamauchi, Toyohiko; Iwai, Hidenao; Yamashita, Yutaka

2011-11-01

We demonstrate tomographic imaging of intracellular activity of living cells by a low-coherent quantitative phase microscope. The intracellular organelles, such as the nucleus, nucleolus, and mitochondria, are moving around inside living cells, driven by the cellular physiological activity. In order to visualize the intracellular motility in a label-free manner we have developed a reflection-type quantitative phase microscope which employs the phase shifting interferometric technique with a low-coherent light source. The phase shifting interferometry enables us to quantitatively measure the intensity and phase of the optical field, and the low-coherence interferometry makes it possible to selectively probe a specific sectioning plane in the cell volume. The results quantitatively revealed the depth-resolved fluctuations of intracellular surfaces so that the plasma membrane and the membranes of intracellular organelles were independently measured. The transversal and the vertical spatial resolutions were 0.56 μm and 0.93 μm, respectively, and the mechanical sensitivity of the phase measurement was 1.2 nanometers. The mean-squared displacement was applied as a statistical tool to analyze the temporal fluctuation of the intracellular organelles. To the best of our knowledge, our system visualized depth-resolved intracellular organelles motion for the first time in sub-micrometer resolution without contrast agents.

Spatially-resolved star formation histories of CALIFA galaxies. Implications for galaxy formation

NASA Astrophysics Data System (ADS)

González Delgado, R. M.; Pérez, E.; Cid Fernandes, R.; García-Benito, R.; López Fernández, R.; Vale Asari, N.; Cortijo-Ferrero, C.; de Amorim, A. L.; Lacerda, E. A. D.; Sánchez, S. F.; Lehnert, M. D.; Walcher, C. J.

2017-11-01

This paper presents the spatially resolved star formation history (SFH) of nearby galaxies with the aim of furthering our understanding of the different processes involved in the formation and evolution of galaxies. To this end, we apply the fossil record method of stellar population synthesis to a rich and diverse data set of 436 galaxies observed with integral field spectroscopy in the CALIFA survey. The sample covers a wide range of Hubble types, with stellar masses ranging from M⋆ 109 to 7 × 1011 M⊙. Spectral synthesis techniques are applied to the datacubes to retrieve the spatially resolved time evolution of the star formation rate (SFR), its intensity (ΣSFR), and other descriptors of the 2D SFH in seven bins of galaxy morphology (E, S0, Sa, Sb, Sbc, Sc, and Sd) and five bins of stellar mass. Our main results are that (a) galaxies form very fast independently of their current stellar mass, with the peak of star formation at high redshift (z > 2). Subsequent star formation is driven by M⋆ and morphology, with less massive and later type spirals showing more prolonged periods of star formation. (b) At any epoch in the past, the SFR is proportional to M⋆, with most massive galaxies having the highest absolute (but lowest specific) SFRs. (c) While today, the ΣSFR is similar for all spirals and significantly lower in early-type galaxies (ETG), in the past, the ΣSFR scales well with morphology. The central regions of today's ETGs are where the ΣSFR reached the highest values (> 103 M⊙ Gyr-1 pc-2), similar to those measured in high-redshift star-forming galaxies. (d) The evolution of ΣSFR in Sbc systems matches that of models for Milky Way-like galaxies, suggesting that the formation of a thick disk may be a common phase in spirals at early epochs. (e) The SFR and ΣSFR in outer regions of E and S0 galaxies show that they have undergone an extended phase of growth in mass between z = 2 and 0.4. The mass assembled in this phase is in agreement with

Spatially resolved XRF, XAFS, XRD, STXM and IR investigation of a natural U-rich clay

NASA Astrophysics Data System (ADS)

Denecke, M. A.; Michel, P.; Schäfer, T.; Huber, F.; Rickers, K.; Rothe, J.; Dardenne, K.; Brendebach, B.; Vitova, T.; Elie, M.

2009-11-01

Combined spatially resolved hard X-ray μ-XRF and μ-XAFS studies using an X-ray beam with micrometer dimensions at the INE-Beamline for actinide research at ANKA and Beamline L at HASYLAB with those from scanning transmission soft X-ray microscopy (STXM) and synchrotron-based Fourier transform infrared microspectroscopy (μ-FTIR) recorded with beam spots in the nanometer range are used to study a U-rich clay originating from Autunian shales in the Permian Lodève Basin (France). This argillaceous formation is a natural U deposit associated with organic matter (bitumen). Results allow us to differentiate between possible mechanisms leading to U enrichment: likely U immobilization via reaction with organic material associated with clay mineral. Such investigations support development of reliable assessment of the long term radiological safety for proposed nuclear waste disposal sites.

Spatially resolving the dust properties and submillimetre excess in M 33

NASA Astrophysics Data System (ADS)

Relaño, M.; De Looze, I.; Kennicutt, R. C.; Lisenfeld, U.; Dariush, A.; Verley, S.; Braine, J.; Tabatabaei, F.; Kramer, C.; Boquien, M.; Xilouris, M.; Gratier, P.

2018-05-01

Context. The relative abundance of the dust grain types in the interstellar medium is directly linked to physical quantities that trace the evolution of galaxies. Because of the poor spatial resolution of the infrared and submillimetre data, we are able to study the dependence of the resolved infrared spectral energy distribution (SED) across regions of the interstellar medium (ISM) with different physical properties in just a few objects. Aims: We aim to study the dust properties of the whole disc of M 33 at spatial scales of 170 pc. This analysis allows us to infer how the relative dust grain abundance changes with the conditions of the ISM, study the existence of a submillimetre excess and look for trends of the gas-to-dust mass ratio (GDR) with other physical properties of the galaxy. Methods: For each pixel in the disc of M 33 we have fitted the infrared SED using a physically motivated dust model that assumes an emissivity index β close to two. We applied a Bayesian statistical method to fit the individual SEDs and derived the best output values from the study of the probability density function of each parameter. We derived the relative amount of the different dust grains in the model, the total dust mass, and the strength of the interstellar radiation field (ISRF) heating the dust at each spatial location. Results: The relative abundance of very small grains tends to increase, and for big grains to decrease, at high values of Hα luminosity. This shows that the dust grains are modified inside the star-forming regions, in agreement with a theoretical framework of dust evolution under different physical conditions. The radial dependence of the GDR is consistent with the shallow metallicity gradient observed in this galaxy. The strength of the ISRF derived in our model correlates with the star formation rate in the galaxy in a pixel by pixel basis. Although this is expected, it is the first time that a correlation between the two quantities has been reported

Quantitative phase imaging of biological cells using spatially low and temporally high coherent light source.

PubMed

Ahmad, Azeem; Dubey, Vishesh; Singh, Gyanendra; Singh, Veena; Mehta, Dalip Singh

2016-04-01

In this Letter, we demonstrate quantitative phase imaging of biological samples, such as human red blood cells (RBCs) and onion cells using narrow temporal frequency and wide angular frequency spectrum light source. This type of light source was synthesized by the combined effect of spatial, angular, and temporal diversity of speckle reduction technique. The importance of using low spatial and high temporal coherence light source over the broad band and narrow band light source is that it does not require any dispersion compensation mechanism for biological samples. Further, it avoids the formation of speckle or spurious fringes which arises while using narrow band light source.

Spatially Resolved Measurements of CO2 and CH4 Concentration and Gas-Exchange Velocity Highly Influence Carbon-Emission Estimates of Reservoirs

PubMed Central

2017-01-01

The magnitude of diffusive carbon dioxide (CO2) and methane (CH4) emission from man-made reservoirs is uncertain because the spatial variability generally is not well-represented. Here, we examine the spatial variability and its drivers for partial pressure, gas-exchange velocity (k), and diffusive flux of CO2 and CH4 in three tropical reservoirs using spatially resolved measurements of both gas concentrations and k. We observed high spatial variability in CO2 and CH4 concentrations and flux within all three reservoirs, with river inflow areas generally displaying elevated CH4 concentrations. Conversely, areas close to the dam are generally characterized by low concentrations and are therefore not likely to be representative for the whole system. A large share (44–83%) of the within-reservoir variability of gas concentration was explained by dissolved oxygen, pH, chlorophyll, water depth, and within-reservoir location. High spatial variability in k was observed, and kCH4 was persistently higher (on average, 2.5 times more) than kCO2. Not accounting for the within-reservoir variability in concentrations and k may lead to up to 80% underestimation of whole-system diffusive emission of CO2 and CH4. Our findings provide valuable information on how to develop field-sampling strategies to reliably capture the spatial heterogeneity of diffusive carbon fluxes from reservoirs. PMID:29257874

A Quantitative Spatial Proteomics Analysis of Proteome Turnover in Human Cells*

PubMed Central

Boisvert, François-Michel; Ahmad, Yasmeen; Gierliński, Marek; Charrière, Fabien; Lamont, Douglas; Scott, Michelle; Barton, Geoff; Lamond, Angus I.

2012-01-01

Measuring the properties of endogenous cell proteins, such as expression level, subcellular localization, and turnover rates, on a whole proteome level remains a major challenge in the postgenome era. Quantitative methods for measuring mRNA expression do not reliably predict corresponding protein levels and provide little or no information on other protein properties. Here we describe a combined pulse-labeling, spatial proteomics and data analysis strategy to characterize the expression, localization, synthesis, degradation, and turnover rates of endogenously expressed, untagged human proteins in different subcellular compartments. Using quantitative mass spectrometry and stable isotope labeling with amino acids in cell culture, a total of 80,098 peptides from 8,041 HeLa proteins were quantified, and their spatial distribution between the cytoplasm, nucleus and nucleolus determined and visualized using specialized software tools developed in PepTracker. Using information from ion intensities and rates of change in isotope ratios, protein abundance levels and protein synthesis, degradation and turnover rates were calculated for the whole cell and for the respective cytoplasmic, nuclear, and nucleolar compartments. Expression levels of endogenous HeLa proteins varied by up to seven orders of magnitude. The average turnover rate for HeLa proteins was ∼20 h. Turnover rate did not correlate with either molecular weight or net charge, but did correlate with abundance, with highly abundant proteins showing longer than average half-lives. Fast turnover proteins had overall a higher frequency of PEST motifs than slow turnover proteins but no general correlation was observed between amino or carboxyl terminal amino acid identities and turnover rates. A subset of proteins was identified that exist in pools with different turnover rates depending on their subcellular localization. This strongly correlated with subunits of large, multiprotein complexes, suggesting a general

Challenges in Scale-Resolving Simulations of turbulent wake flows with coherent structures

NASA Astrophysics Data System (ADS)

Pereira, Filipe S.; Eça, Luís; Vaz, Guilherme; Girimaji, Sharath S.

2018-06-01

The objective of this work is to investigate the challenges encountered in Scale-Resolving Simulations (SRS) of turbulent wake flows driven by spatially-developing coherent structures. SRS of practical interest are expressly intended for efficiently computing such flows by resolving only the most important features of the coherent structures and modelling the remainder as stochastic field. The success of SRS methods depends upon three important factors: i) ability to identify key flow mechanisms responsible for the generation of coherent structures; ii) determine the optimum range of resolution required to adequately capture key elements of coherent structures; and iii) ensure that the modelled part is comprised nearly exclusively of fully-developed stochastic turbulence. This study considers the canonical case of the flow around a circular cylinder to address the aforementioned three key issues. It is first demonstrated using experimental evidence that the vortex-shedding instability and flow-structure development involves four important stages. A series of SRS computations of progressively increasing resolution (decreasing cut-off length) are performed. An a priori basis for locating the origin of the coherent structures development is proposed and examined. The criterion is based on the fact that the coherent structures are generated by the Kelvin-Helmholtz (KH) instability. The most important finding is that the key aspects of coherent structures can be resolved only if the effective computational Reynolds number (based on total viscosity) exceeds the critical value of the KH instability in laminar flows. Finally, a quantitative criterion assessing the nature of the unresolved field based on the strain-rate ratio of mean and unresolved fields is examined. The two proposed conditions and rationale offer a quantitative basis for developing "good practice" guidelines for SRS of complex turbulent wake flows with coherent structures.

A microreactor array for spatially resolved measurement of catalytic activity for high-throughput catalysis science

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kondratyuk, Petro; Gumuslu, Gamze; Shukla, Shantanu

2013-04-01

We describe a 100 channel microreactor array capable of spatially resolved measurement of catalytic activity across the surface of a flat substrate. When used in conjunction with a composition spread alloy film (CSAF, e.g. Pd{sub x}Cu{sub y}Au{sub 1-x-y}) across which component concentrations vary smoothly, such measurements permit high-throughput analysis of catalytic activity and selectivity as a function of catalyst composition. In the reported implementation, the system achieves spatial resolution of 1 mm{sup 2} over a 10×10 mm{sup 2} area. During operation, the reactant gases are delivered at constant flow rate to 100 points of differing composition on the CSAF surfacemore » by means of a 100-channel microfluidic device. After coming into contact with the CSAF catalyst surface, the product gas mixture from each of the 100 points is withdrawn separately through a set of 100 isolated channels for analysis using a mass spectrometer. We demonstrate the operation of the device on a Pd{sub x}Cu{sub y}Au{sub 1-x-y} CSAF catalyzing the H{sub 2}-D{sub 2} exchange reaction at 333 K. In essentially a single experiment, we measured the catalytic activity over a broad swathe of concentrations from the ternary composition space of the Pd{sub x}Cu{sub y}Au{sub 1-x-y} alloy.« less

SPATIALLY RESOLVED HCN ABSORPTION FEATURES IN THE CIRCUMNUCLEAR REGION OF NGC 1052

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sawada-Satoh, Satoko; Roh, Duk-Gyoo; Oh, Se-Jin

We present the first VLBI detection of HCN molecular absorption in the nearby active galactic nucleus NGC 1052. Utilizing the 1 mas resolution achieved by the Korean VLBI Network, we have spatially resolved the HCN absorption against a double-sided nuclear jet structure. Two velocity features of HCN absorption are detected significantly at the radial velocity of 1656 and 1719 km s{sup −1}, redshifted by 149 and 212 km s{sup −1} with respect to the systemic velocity of the galaxy. The column density of the HCN molecule is estimated to be 10{sup 15}–10{sup 16} cm{sup −2}, assuming an excitation temperature ofmore » 100–230 K. The absorption features show high optical depth localized on the receding jet side, where the free–free absorption occurred due to the circumnuclear torus. The size of the foreground absorbing molecular gas is estimated to be on approximately one-parsec scales, which agrees well with the approximate size of the circumnuclear torus. HCN absorbing gas is likely to be several clumps smaller than 0.1 pc inside the circumnuclear torus. The redshifted velocities of the HCN absorption features imply that HCN absorbing gas traces ongoing infall motion inside the circumnuclear torus onto the central engine.« less

Research needs for finely resolved fossil carbon emissions

USGS Publications Warehouse

Gurney, K.; Ansley, W.; Mendoza, D.; Petron, G.; Frost, G.; Gregg, J.; Fischer, M.; Pataki, Diane E.; Ackerman, K.; Houweling, S.; Corbin, K.; Andres, R.; Blasing, T.J.

2007-01-01

Scientific research on the global carbon cycle has emerged as a high priority in biogeochemistry, climate studies, and global change policy. The emission of carbon dioxide (CO2) from fossil fuel combustion is a dominant driver of the current net carbon fluxes between the land, the oceans, and the atmosphere, and it is a key contributor to the rise in modern radiative forcing. Contrary to a commonly held perception, our quantitative knowledge about these emissions is insufficient to satisfy current scientific and policy needs. A more highly spatially and temporally resolved quantification of the social and economic drivers of fossil fuel combustion, and the resulting CO2 emissions, is essential to supporting scientific and policy progress. In this article, a new community of emissions researchers called the CO2 Fossil Fuel Emission Effort (CO2FFEE) outlines a research agenda to meet the need for improved fossil fuel CO2 emissions information and solicits comment from the scientific community and research agencies.

Spatially Resolved Emission of a z~3 Damped Lyman Alpha Galaxy with Keck/OSIRIS IFU

NASA Astrophysics Data System (ADS)

Christenson, Holly; Jorgenson, Regina

2017-01-01

The damped Lyman alpha (DLA) class of galaxies contains most of the neutral hydrogen gas over cosmic time. Few DLAs have been detected directly, which limits our knowledge of fundamental properties like size and mass. We present Keck/OSIRIS infrared integral field spectroscopy (IFU) observations of a DLA that was first detected in absorption toward a background quasar. Our observations use the Keck Laser Guide Star Adaptive Optics system to reduce the point-spread function of the quasar, making it possible to spatially resolve the DLA emission. We map this emission in O[III] 5007 Å. At redshift z~3, this DLA represents one of the highest redshift DLAs mapped with IFU spectroscopy. We present measurements of the star formation rate, metallicity, and gas mass of the galaxy.This project was supported in part by the NSF REU grant AST-1358980 and by the Nantucket Maria Mitchell Association.

Assessment of swirl spray interaction in lab scale combustor using time-resolved measurements

NASA Astrophysics Data System (ADS)

Rajamanickam, Kuppuraj; Jain, Manish; Basu, Saptarshi

2017-11-01

Liquid fuel injection in highly turbulent swirling flows becomes common practice in gas turbine combustors to improve the flame stabilization. It is well known that the vortex bubble breakdown (VBB) phenomenon in strong swirling jets exhibits complicated flow structures in the spatial domain. In this study, the interaction of hollow cone liquid sheet with such coaxial swirling flow field has been studied experimentally using time-resolved measurements. In particular, much attention is focused towards the near field breakup mechanism (i.e. primary atomization) of liquid sheet. The detailed swirling gas flow field characterization is carried out using time-resolved PIV ( 3.5 kHz). Furthermore, the complicated breakup mechanisms and interaction of the liquid sheet are imaged with the help of high-speed shadow imaging system. Subsequently, proper orthogonal decomposition (POD) and dynamic mode decomposition (DMD) is implemented over the instantaneous data sets to retrieve the modal information associated with the interaction dynamics. This helps to delineate more quantitative nature of interaction process between the liquid sheet and swirling gas phase flow field.

Comparison of grain to grain orientation and stiffness mapping by spatially resolved acoustic spectroscopy and EBSD.

PubMed

Mark, A F; Li, W; Sharples, S; Withers, P J

2017-07-01

Our aim was to establish the capability of spatially resolved acoustic spectroscopy (SRAS) to map grain orientations and the anisotropy in stiffness at the sub-mm to micron scale by comparing the method with electron backscatter diffraction (EBSD) undertaken within a scanning electron microscope. In the former the grain orientations are deduced by measuring the spatial variation in elastic modulus; conversely, in EBSD the elastic anisotropy is deduced from direct measurements of the crystal orientations. The two test-cases comprise mapping the fusion zones for large TIG and MMA welds in thick power plant austenitic and ferritic steels, respectively; these are technologically important because, among other things, elastic anisotropy can cause ultrasonic weld inspection methods to become inaccurate because it causes bending in the paths of sound waves. The spatial resolution of SRAS is not as good as that for EBSD (∼100 μm vs. ∼a few nm), nor is the angular resolution (∼1.5° vs. ∼0.5°). However the method can be applied to much larger areas (currently on the order of 300 mm square), is much faster (∼5 times), is cheaper and easier to perform, and it could be undertaken on the manufacturing floor. Given these advantages, particularly to industrial users, and the on-going improvements to the method, SRAS has the potential to become a standard method for orientation mapping, particularly in cases where the elastic anisotropy is important over macroscopic/component length scales. © 2017 The Authors Journal of Microscopy © 2017 Royal Microscopical Society.

Portable measurement system for real-time acquisition and analysis of in-vivo spatially resolved reflectance in the subdiffusive regime

NASA Astrophysics Data System (ADS)

Naglič, Peter; Ivančič, Matic; Pernuš, Franjo; Likar, Boštjan; Bürmen, Miran

2018-02-01

A measurement system was developed to acquire and analyze subdiffusive spatially resolved reflectance using an optical fiber probe with short source-detector separations. Since subdiffusive reflectance significantly depends on the scattering phase function, the analysis of the acquired reflectance is based on a novel inverse Monte Carlo model that allows estimation of phase function related parameters in addition to the absorption and reduced scattering coefficients. In conjunction with our measurement system, the model allowed real-time estimation of optical properties, which we demonstrate for a case of dynamically induced changes in human skin by applying pressure with an optical fiber probe.

Global, quantitative and dynamic mapping of protein subcellular localization.

PubMed

Itzhak, Daniel N; Tyanova, Stefka; Cox, Jürgen; Borner, Georg Hh

2016-06-09

Subcellular localization critically influences protein function, and cells control protein localization to regulate biological processes. We have developed and applied Dynamic Organellar Maps, a proteomic method that allows global mapping of protein translocation events. We initially used maps statically to generate a database with localization and absolute copy number information for over 8700 proteins from HeLa cells, approaching comprehensive coverage. All major organelles were resolved, with exceptional prediction accuracy (estimated at >92%). Combining spatial and abundance information yielded an unprecedented quantitative view of HeLa cell anatomy and organellar composition, at the protein level. We subsequently demonstrated the dynamic capabilities of the approach by capturing translocation events following EGF stimulation, which we integrated into a quantitative model. Dynamic Organellar Maps enable the proteome-wide analysis of physiological protein movements, without requiring any reagents specific to the investigated process, and will thus be widely applicable in cell biology.

Quantitative measurement of cerebral blood flow in a juvenile porcine model by depth-resolved near-infrared spectroscopy

NASA Astrophysics Data System (ADS)

Elliott, Jonathan T.; Diop, Mamadou; Tichauer, Kenneth M.; Lee, Ting-Yim; Lawrence, Keith St.

2010-05-01

Nearly half a million children and young adults are affected by traumatic brain injury each year in the United States. Although adequate cerebral blood flow (CBF) is essential to recovery, complications that disrupt blood flow to the brain and exacerbate neurological injury often go undetected because no adequate bedside measure of CBF exists. In this study we validate a depth-resolved, near-infrared spectroscopy (NIRS) technique that provides quantitative CBF measurement despite significant signal contamination from skull and scalp tissue. The respiration rates of eight anesthetized pigs (weight: 16.2+/-0.5 kg, age: 1 to 2 months old) are modulated to achieve a range of CBF levels. Concomitant CBF measurements are performed with NIRS and CT perfusion. A significant correlation between CBF measurements from the two techniques is demonstrated (r2=0.714, slope=0.92, p<0.001), and the bias between the two techniques is -2.83 mL.min-1.100 g-1 (CI0.95: -19.63 mL.min-1.100 g-1-13.9 mL.min-1.100 g-1). This study demonstrates that accurate measurements of CBF can be achieved with depth-resolved NIRS despite significant signal contamination from scalp and skull. The ability to measure CBF at the bedside provides a means of detecting, and thereby preventing, secondary ischemia during neurointensive care.

An online, energy-resolving beam profile detector for laser-driven proton beams.

PubMed

Metzkes, J; Zeil, K; Kraft, S D; Karsch, L; Sobiella, M; Rehwald, M; Obst, L; Schlenvoigt, H-P; Schramm, U

2016-08-01

In this paper, a scintillator-based online beam profile detector for the characterization of laser-driven proton beams is presented. Using a pixelated matrix with varying absorber thicknesses, the proton beam is spatially resolved in two dimensions and simultaneously energy-resolved. A thin plastic scintillator placed behind the absorber and read out by a CCD camera is used as the active detector material. The spatial detector resolution reaches down to ∼4 mm and the detector can resolve proton beam profiles for up to 9 proton threshold energies. With these detector design parameters, the spatial characteristics of the proton distribution and its cut-off energy can be analyzed online and on-shot under vacuum conditions. The paper discusses the detector design, its characterization and calibration at a conventional proton source, as well as the first detector application at a laser-driven proton source.

Online, absolute quantitation of propranolol from spatially distinct 20-μm and 40-μm dissections of brain, liver, and kidney thin tissue sections by laser microdissection – liquid vortex capture – mass spectrometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kertesz, Vilmos; Vavrek, Marissa; Freddo, Carol

Here, spatial resolved quantitation of chemical species in thin tissue sections by mass spectrometric methods has been constrained by the need for matrix-matched standards or other arduous calibration protocols and procedures to mitigate matrix effects (e.g., spatially varying ionization suppression). Reported here is the use of laser cut and drop sampling with a laser microdissection-liquid vortex capture electrospray ionization tandem mass spectrometry (LMD-LVC/ESI-MS/MS) system for online and absolute quantitation of propranolol in mouse brain, kidney, and liver thin tissue sections of mice administered with the drug at a 7.5 mg/kg dose, intravenously. In this procedure either 20 μm x 20more » μm or 40 μm x 40 μm tissue microdissections were cut and dropped into the flowing solvent of the capture probe. During transport to the ESI source drug related material was completely extracted from the tissue into the solvent, which contained a known concentration of propranolol-d 7 as an internal standard. This allowed absolute quantitation to be achieved with an external calibration curve generated from standards containing the same fixed concentration of propranolold-d 7 and varied concentrations of propranolol. Average propranolol concentrations determined with the laser cut and drop sampling method closely agreed with concentration values obtained from 2.3 mm diameter tissue punches from serial sections that were extracted and quantified by HPLC/ESI-MS/MS measurements. In addition, the relative abundance of hydroxypropranolol glucuronide metabolites were recorded and found to be consistent with previous findings.« less

Online, absolute quantitation of propranolol from spatially distinct 20-μm and 40-μm dissections of brain, liver, and kidney thin tissue sections by laser microdissection – liquid vortex capture – mass spectrometry

DOE PAGES

Kertesz, Vilmos; Vavrek, Marissa; Freddo, Carol; ...

2016-05-23

Here, spatial resolved quantitation of chemical species in thin tissue sections by mass spectrometric methods has been constrained by the need for matrix-matched standards or other arduous calibration protocols and procedures to mitigate matrix effects (e.g., spatially varying ionization suppression). Reported here is the use of laser cut and drop sampling with a laser microdissection-liquid vortex capture electrospray ionization tandem mass spectrometry (LMD-LVC/ESI-MS/MS) system for online and absolute quantitation of propranolol in mouse brain, kidney, and liver thin tissue sections of mice administered with the drug at a 7.5 mg/kg dose, intravenously. In this procedure either 20 μm x 20more » μm or 40 μm x 40 μm tissue microdissections were cut and dropped into the flowing solvent of the capture probe. During transport to the ESI source drug related material was completely extracted from the tissue into the solvent, which contained a known concentration of propranolol-d 7 as an internal standard. This allowed absolute quantitation to be achieved with an external calibration curve generated from standards containing the same fixed concentration of propranolold-d 7 and varied concentrations of propranolol. Average propranolol concentrations determined with the laser cut and drop sampling method closely agreed with concentration values obtained from 2.3 mm diameter tissue punches from serial sections that were extracted and quantified by HPLC/ESI-MS/MS measurements. In addition, the relative abundance of hydroxypropranolol glucuronide metabolites were recorded and found to be consistent with previous findings.« less

Quantitative measurement of piezoelectric coefficient of thin film using a scanning evanescent microwave microscope.

PubMed

Zhao, Zhenli; Luo, Zhenlin; Liu, Chihui; Wu, Wenbin; Gao, Chen; Lu, Yalin

2008-06-01

This article describes a new approach to quantitatively measure the piezoelectric coefficients of thin films at the microscopic level using a scanning evanescent microwave microscope. This technique can resolve 10 pm deformation caused by the piezoelectric effect and has the advantages of high scanning speed, large scanning area, submicron spatial resolution, and a simultaneous accessibility to many other related properties. Results from the test measurements on the longitudinal piezoelectric coefficient of PZT thin film agree well with those from other techniques listed in literatures.

Introduction to Time-Resolved Spectroscopy: Nanosecond Transient Absorption and Time-Resolved Fluorescence of Eosin B

ERIC Educational Resources Information Center

Farr, Erik P.; Quintana, Jason C.; Reynoso, Vanessa; Ruberry, Josiah D.; Shin, Wook R.; Swartz, Kevin R.

2018-01-01

Here we present a new undergraduate laboratory that will introduce the concepts of time-resolved spectroscopy and provide insight into the natural time scales on which chemical dynamics occur through direct measurement. A quantitative treatment of the acquired data will provide a deeper understanding of the role of quantum mechanics and various…

A SCR Model Calibration Approach with Spatially Resolved Measurements and NH 3 Storage Distributions

DOE PAGES

Song, Xiaobo; Parker, Gordon G.; Johnson, John H.; ...

2014-11-27

The selective catalytic reduction (SCR) is a technology used for reducing NO x emissions in the heavy-duty diesel (HDD) engine exhaust. In this study, the spatially resolved capillary inlet infrared spectroscopy (Spaci-IR) technique was used to study the gas concentration and NH 3 storage distributions in a SCR catalyst, and to provide data for developing a SCR model to analyze the axial gaseous concentration and axial distributions of NH 3 storage. A two-site SCR model is described for simulating the reaction mechanisms. The model equations and a calculation method was developed using the Spaci-IR measurements to determine the NH 3more » storage capacity and the relationships between certain kinetic parameters of the model. Moreover, a calibration approach was then applied for tuning the kinetic parameters using the spatial gaseous measurements and calculated NH3 storage as a function of axial position instead of inlet and outlet gaseous concentrations of NO, NO 2, and NH 3. The equations and the approach for determining the NH 3 storage capacity of the catalyst and a method of dividing the NH 3 storage capacity between the two storage sites are presented. It was determined that the kinetic parameters of the adsorption and desorption reactions have to follow certain relationships for the model to simulate the experimental data. Finally, the modeling results served as a basis for developing full model calibrations to SCR lab reactor and engine data and state estimator development as described in the references (Song et al. 2013a, b; Surenahalli et al. 2013).« less

Spatially resolved micro-Raman observation on the phase separation of effloresced sea salt droplets.

PubMed

Xiao, Han-Shuang; Dong, Jin-Ling; Wang, Liang-Yu; Zhao, Li-Jun; Wang, Feng; Zhang, Yun-Hong

2008-12-01

We report on the investigation of the phase separation of individual seawater droplets in the efflorescence processes with the spatially resolved Raman system. Upon decreasing the relative humidity (RH), CaSO4.0.5H2O separated out foremost fromthe droplet atan unexpectedly high RH of approcimately 90%. Occasionally, CaSO4.2H2O substituted for CaSO4.O.5H2O crystallizing first at approximately 78% RH. Relatively large NaCI solids followed to crystallize at approximately 55% RH and led to the great loss of the solution. Then, the KMgCl3.6H2O crystallites separated out from the residual solutions, adjacentto NaCl at approximately 44% RH. Moreover, a shell structure of dried sea salt particle was found to form at low RHs, with the NaCl crystals in the core and minor supersaturated solutions covered with MgSO4 gel coating on the surface. Ultimately, the shielded solution partly effloresced into MgSO4 hydrates at very dry state (<5% RH).

Spatially resolved assessment of hepatic function using 99mTc-IDA SPECT

PubMed Central

Wang, Hesheng; Cao, Yue

2013-01-01

Purpose: 99mTc-iminodiacetic acid (IDA) hepatobiliary imaging is usually quantified for hepatic function on the entire liver or regions of interest (ROIs) in the liver. The authors presented a method to estimate the hepatic extraction fraction (HEF) voxel-by-voxel from single-photon emission computed tomography (SPECT)/CT with a 99mTc-labeled IDA agent of mebrofenin and evaluated the spatially resolved HEF measurements with an independent physiological measurement. Methods: Fourteen patients with intrahepatic cancers were treated with radiation therapy (RT) and imaged by 99mTc-mebrofenin SPECT before and 1 month after RT. The dynamic SPECT volumes were with a resolution of 3.9 × 3.9 × 2.5 mm3. Throughout the whole liver with approximate 50 000 voxels, voxelwise HEF quantifications were estimated and compared between using arterial input function (AIF) from the heart and using vascular input function (VIF) from the spleen. The correlation between mean of the HEFs over the nontumor liver tissue and the overall liver function measured by Indocyanine green clearance half-time (T1/2) was assessed. Variation of the voxelwise estimation was evaluated in ROIs drawn in relatively homogeneous regions of the livers. The authors also examined effects of the time range parameter on the voxelwise HEF quantification. Results: Mean of the HEFs over the liver estimated using AIF significantly correlated with the physiological measurement T1/2 (r = 0.52, p = 0.0004), and the correlation was greatly improved by using VIF (r = 0.79, p < 0.0001). The parameter of time range for the retention phase did not lead to a significant difference in the means of the HEFs in the ROIs. Using VIF and a retention phase time range of 7–30 min, the relative variation of the voxelwise HEF in the ROIs was 10% ± 6% of respective mean HEF. Conclusions: The voxelwise HEF derived from 99mTc-IDA SPECT by the deconvolution analysis is feasible to assess the spatial distribution of hepatic function in

Broadband interferometric characterization of divergence and spatial chirp.

PubMed

Meier, Amanda K; Iliev, Marin; Squier, Jeff A; Durfee, Charles G

2015-09-01

We demonstrate a spectral interferometric method to characterize lateral and angular spatial chirp to optimize intensity localization in spatio-temporally focused ultrafast beams. Interference between two spatially sheared beams in an interferometer will lead to straight fringes if the wavefronts are curved. To produce reference fringes, we delay one arm relative to another in order to measure fringe rotation in the spatially resolved spectral interferogram. With Fourier analysis, we can obtain frequency-resolved divergence. In another arrangement, we spatially flip one beam relative to the other, which allows the frequency-dependent beamlet direction (angular spatial chirp) to be measured. Blocking one beam shows the spatial variation of the beamlet position with frequency (i.e., the lateral spatial chirp).

Spatially and temporally resolved measurements of a dense copper plasma heated by intense relativistic electrons

NASA Astrophysics Data System (ADS)

Coleman, J. E.; Colgan, J.

2017-08-01

A 100-μm-thick Cu foil is isochorically heated by a ˜100-ns-long electron bunch with an energy of 19.8 MeV and current of 1.7 kA to Te > 1 eV. After 100 ns of heating and 20 ns of expansion, the plasma exhibits a stable, quiescent temperature and density distribution for >200 ns. Several intense Cu-I emission lines are observed after ˜20 J of electron beam energy is deposited. These lines have well known Stark widths providing a direct measurement of ne. The Los Alamos ATOMIC code [Magee et al., AIP Conf. Proc. 2004, 168-179 and Hakel et al., J. Quant. Spectrosc. Radiat. Transfer 99, 265 (2006)] was run in local-thermodynamic-equilibrium mode to estimate Te and ne. Spatially and temporally resolved measurements are presented in both the vertical and horizontal directions adjacent to the foil indicating temperatures >1 eV and densities ranging from 1-3 × 1017 cm-3 after expansion and cooling.

An online, energy-resolving beam profile detector for laser-driven proton beams

DOE Office of Scientific and Technical Information (OSTI.GOV)

Metzkes, J.; Rehwald, M.; Obst, L.

In this paper, a scintillator-based online beam profile detector for the characterization of laser-driven proton beams is presented. Using a pixelated matrix with varying absorber thicknesses, the proton beam is spatially resolved in two dimensions and simultaneously energy-resolved. A thin plastic scintillator placed behind the absorber and read out by a CCD camera is used as the active detector material. The spatial detector resolution reaches down to ∼4 mm and the detector can resolve proton beam profiles for up to 9 proton threshold energies. With these detector design parameters, the spatial characteristics of the proton distribution and its cut-off energymore » can be analyzed online and on-shot under vacuum conditions. The paper discusses the detector design, its characterization and calibration at a conventional proton source, as well as the first detector application at a laser-driven proton source.« less

Towards real-time non contact spatial resolved oxygenation monitoring using a multi spectral filter array camera in various light conditions

NASA Astrophysics Data System (ADS)

Bauer, Jacob R.; van Beekum, Karlijn; Klaessens, John; Noordmans, Herke Jan; Boer, Christa; Hardeberg, Jon Y.; Verdaasdonk, Rudolf M.

2018-02-01

Non contact spatial resolved oxygenation measurements remain an open challenge in the biomedical field and non contact patient monitoring. Although point measurements are the clinical standard till this day, regional differences in the oxygenation will improve the quality and safety of care. Recent developments in spectral imaging resulted in spectral filter array cameras (SFA). These provide the means to acquire spatial spectral videos in real-time and allow a spatial approach to spectroscopy. In this study, the performance of a 25 channel near infrared SFA camera was studied to obtain spatial oxygenation maps of hands during an occlusion of the left upper arm in 7 healthy volunteers. For comparison a clinical oxygenation monitoring system, INVOS, was used as a reference. In case of the NIRS SFA camera, oxygenation curves were derived from 2-3 wavelength bands with a custom made fast analysis software using a basic algorithm. Dynamic oxygenation changes were determined with the NIR SFA camera and INVOS system at different regional locations of the occluded versus non-occluded hands and showed to be in good agreement. To increase the signal to noise ratio, algorithm and image acquisition were optimised. The measurement were robust to different illumination conditions with NIR light sources. This study shows that imaging of relative oxygenation changes over larger body areas is potentially possible in real time.

Spatially resolved regression analysis of pre-treatment FDG, FLT and Cu-ATSM PET from post-treatment FDG PET: an exploratory study

PubMed Central

Bowen, Stephen R; Chappell, Richard J; Bentzen, Søren M; Deveau, Michael A; Forrest, Lisa J; Jeraj, Robert

2012-01-01

Purpose To quantify associations between pre-radiotherapy and post-radiotherapy PET parameters via spatially resolved regression. Materials and methods Ten canine sinonasal cancer patients underwent PET/CT scans of [18F]FDG (FDGpre), [18F]FLT (FLTpre), and [61Cu]Cu-ATSM (Cu-ATSMpre). Following radiotherapy regimens of 50 Gy in 10 fractions, veterinary patients underwent FDG PET/CT scans at three months (FDGpost). Regression of standardized uptake values in baseline FDGpre, FLTpre and Cu-ATSMpre tumour voxels to those in FDGpost images was performed for linear, log-linear, generalized-linear and mixed-fit linear models. Goodness-of-fit in regression coefficients was assessed by R2. Hypothesis testing of coefficients over the patient population was performed. Results Multivariate linear model fits of FDGpre to FDGpost were significantly positive over the population (FDGpost~0.17 FDGpre, p=0.03), and classified slopes of RECIST non-responders and responders to be different (0.37 vs. 0.07, p=0.01). Generalized-linear model fits related FDGpre to FDGpost by a linear power law (FDGpost~FDGpre0.93, p<0.001). Univariate mixture model fits of FDGpre improved R2 from 0.17 to 0.52. Neither baseline FLT PET nor Cu-ATSM PET uptake contributed statistically significant multivariate regression coefficients. Conclusions Spatially resolved regression analysis indicates that pre-treatment FDG PET uptake is most strongly associated with three-month post-treatment FDG PET uptake in this patient population, though associations are histopathology-dependent. PMID:22682748

Spatially resolved H2 emission from the disk around T Tau N

NASA Astrophysics Data System (ADS)

Gustafsson, M.; Labadie, L.; Herbst, T. M.; Kasper, M.

2008-09-01

Context: Molecular hydrogen is the main constituent of circumstellar disks and could be an important tracer for the evolution and structure of such disks. So far, H2 has only been detected in a few disks and only through spectroscopic observations, resulting in a limited knowledge of the spatial distribution of the H2 emitting gas. Aims: We report the detection of quiescent H2 emission in a spatially resolved ring-like structure within 100 AU of T Tau N. We present evidence to show that the emission most likely arises from shocks in the atmosphere of a nearly face-on disk around T Tau N. Methods: Using high spatial resolution 3D spectroscopic K-band data, we trace the spatial distribution of several H2 NIR rovibrational lines in the vicinity of T Tau N. We examine the structure of the circumstellar material around the star through SED modeling. Then, we use models of shocks and UV+X-ray irradiation to reproduce the H2 line flux and line ratios in order to test how the H2 is excited. Results: We detect weak H2 emission from the v=1{-}0 S(0), S(1), Q(1) lines and the v=2{-}1 S(1) line in a ring-like structure around T Tau N between 0.1 arcsec ( 15 AU) and 0.7 arcsec ( 100 AU) from the star. The v=1{-}0 S(0) and v=2{-}1 S(1) lines are detected only in the outer parts of the ring structure. Closer to the star, the strong continuum limits our sensitivity to these lines. The total flux of the v=1{-}0 S(1) line is 1.8 × 10-14 erg s-1 cm-2, similar to previous measurements of H2 in circumstellar disks. The velocity of the H2 emitting gas around T Tau N is consistent with the rest velocity of the star, and the H2 does not seem to be part of a collimated outflow. Both shocks impinging on the surface of a disk and irradiation of a disk by UV-photons and X-rays from the central star are plausible candidates for the H2 excitation mechanism. However, irradiation should not create a large degree of excitation at radii larger than 20 AU. Most likely the H2 emission arises in the

Longitudinal spatial coherence gated high-resolution tomography and quantitative phase microscopy of biological cells and tissues with uniform illumination

NASA Astrophysics Data System (ADS)

Mehta, Dalip Singh; Ahmad, Azeem; Dubey, Vishesh; Singh, Veena; Butola, Ankit; Mohanty, Tonmoy; Nandi, Sreyankar

2018-02-01

We report longitudinal spatial coherence (LSC) gated high-resolution tomography and quantitative phase microscopy of biological cells and tissues with uniform illumination using laser as a light source. To accomplish this a pseudo thermal light source was synthesized by passing laser beams through an optical system, which is basically a speckle reduction system with combined effect of spatial, temporal, angular and polarisation diversity. The longitudinal spatial coherence length of such light was significantly reduced by synthesizing a pseudo thermal source with the combined effect of spatial, angular and temporal diversity. This results in a low spatially coherent (i.e., broad angular frequency spectrum) light source with narrow temporal frequency spectrum. Light from such a pseudo thermal light source was passed through an interference microscope with varying magnification, such as, 10X and 50X. The interference microscope was used for full-field OCT imaging of multilayer objects and topography of industrial objects. Experimental results of optical sectioning of multilayer biological objects with high axial-resolution less than 10μm was achieved which is comparable to broadband white light source. The synthesized light source with reduced speckles having uniform illumination on the sample, which can be very useful for fluorescence microscopy as well as quantitative phase microscopy with less phase noise. The present system does not require any dispersion compensation optical system for biological samples as a highly monochromatic light source is used.

Accuracy improvement of quantitative analysis by spatial confinement in laser-induced breakdown spectroscopy.

PubMed

Guo, L B; Hao, Z Q; Shen, M; Xiong, W; He, X N; Xie, Z Q; Gao, M; Li, X Y; Zeng, X Y; Lu, Y F

2013-07-29

To improve the accuracy of quantitative analysis in laser-induced breakdown spectroscopy, the plasma produced by a Nd:YAG laser from steel targets was confined by a cavity. A number of elements with low concentrations, such as vanadium (V), chromium (Cr), and manganese (Mn), in the steel samples were investigated. After the optimization of the cavity dimension and laser fluence, significant enhancement factors of 4.2, 3.1, and 2.87 in the emission intensity of V, Cr, and Mn lines, respectively, were achieved at a laser fluence of 42.9 J/cm(2) using a hemispherical cavity (diameter: 5 mm). More importantly, the correlation coefficient of the V I 440.85/Fe I 438.35 nm was increased from 0.946 (without the cavity) to 0.981 (with the cavity); and similar results for Cr I 425.43/Fe I 425.08 nm and Mn I 476.64/Fe I 492.05 nm were also obtained. Therefore, it was demonstrated that the accuracy of quantitative analysis with low concentration elements in steel samples was improved, because the plasma became uniform with spatial confinement. The results of this study provide a new pathway for improving the accuracy of quantitative analysis of LIBS.

Global, quantitative and dynamic mapping of protein subcellular localization

PubMed Central

Itzhak, Daniel N; Tyanova, Stefka; Cox, Jürgen; Borner, Georg HH

2016-01-01

Subcellular localization critically influences protein function, and cells control protein localization to regulate biological processes. We have developed and applied Dynamic Organellar Maps, a proteomic method that allows global mapping of protein translocation events. We initially used maps statically to generate a database with localization and absolute copy number information for over 8700 proteins from HeLa cells, approaching comprehensive coverage. All major organelles were resolved, with exceptional prediction accuracy (estimated at >92%). Combining spatial and abundance information yielded an unprecedented quantitative view of HeLa cell anatomy and organellar composition, at the protein level. We subsequently demonstrated the dynamic capabilities of the approach by capturing translocation events following EGF stimulation, which we integrated into a quantitative model. Dynamic Organellar Maps enable the proteome-wide analysis of physiological protein movements, without requiring any reagents specific to the investigated process, and will thus be widely applicable in cell biology. DOI: http://dx.doi.org/10.7554/eLife.16950.001 PMID:27278775

Spatial-resolved electrochemiluminescence ratiometry based on bipolar electrode for bioanalysis.

PubMed

Wang, Yin-Zhu; Zhao, Wei; Dai, Pan-Pan; Lu, Hai-Jie; Xu, Jing-Juan; Pan, Jing; Chen, Hong-Yuan

2016-12-15

Herein, a spatial-resolved electrochemiluminescene (ECL) ratiometry based on a closed biopolar electrode (BPE) is reported for the highly sensitive detection of prostate specific antigen (PSA). Au@g-C3N4 NCs as one ECL emitter were firstly coated on the cathode of BPE, while the anode of the BPE served for calibration via another ECL substance, Ru(bpy)3(2+). The electroneutrality across the BPE makes the reactions on each pole of BPE electrically coupled. Thus one electrochemical sensing reaction at one pole of BPE could be quantified at both ends. A composite, Pt-PAMAM-DNAzyme was assembled on the surface of cathode via DNA hybridization between probe DNA and PSA aptamer. It acted as an ECL quencher of g-C3N4 via resonance energy transfer (RET) and catalyzing the reduction of O2, the co-reactant of g-C3N4. Meanwhile, it could promote the ECL of Ru(bpy)3(2+) at anode, since the catalytic reduction of O2 at the cathode increased the faradiac current flowing through the BPE. Based on this signal composite, an ECL "off-on" phenomenon was observed at the cathode, after Pt-PAMAM-DNAzyme was "peeled off" by PSA. Conversely, at the anode, an "on-off" ECL changing was obtained. Therefore, a sensitive ratiometry for PSA detection was achieved with a linear range from 0.10 to 200ng/mL. Since the two ECL emitters were physically separated, the ratiometric system was relatively simple and neither optical filters nor spectrometer were required. The strategy combining the ECL ratiometry and BPE broadens the applications of BPE-ECL and shows good perspective in clinical application. Copyright © 2016 Elsevier B.V. All rights reserved.

Spatially resolved variations in reflectivity across iron oxide thin films

NASA Astrophysics Data System (ADS)

Kelley, Chris S.; Thompson, Sarah M.; Gilks, Daniel; Sizeland, James; Lari, Leonardo; Lazarov, Vlado K.; Matsuzaki, Kosuke; LeFrançois, Stéphane; Cinque, Gianfelice; Dumas, Paul

2017-11-01

The spin polarising properties of the iron oxide magnetite (Fe3O4) make it attractive for use in spintronic devices, but its sensitivity to compositional and structural variations make it challenging to prepare reliably. Infrared microspectroscopy and modelling are used to determine the spatial variation in the chemical composition of three thin films of iron oxide; one prepared by pulsed laser deposition (PLD), one by molecular beam epitaxy (MBE) deposition of iron whilst simultaneously flowing oxygen into the chamber and one by flowing oxygen only once deposition is complete. The technique is easily able to distinguish between films which contain metallic iron and different iron oxide phases as well as spatial variations in composition across the films. The film grown by post-oxidising iron is spatially uniform but not fully oxidised, the film grown by simultaneously oxidising iron showed spatial variation in oxide composition while the film grown by PLD was spatially uniform magnetite.

Easy way to determine quantitative spatial resolution distribution for a general inverse problem

NASA Astrophysics Data System (ADS)

An, M.; Feng, M.

2013-12-01

The spatial resolution computation of a solution was nontrivial and more difficult than solving an inverse problem. Most geophysical studies, except for tomographic studies, almost uniformly neglect the calculation of a practical spatial resolution. In seismic tomography studies, a qualitative resolution length can be indicatively given via visual inspection of the restoration of a synthetic structure (e.g., checkerboard tests). An effective strategy for obtaining quantitative resolution length is to calculate Backus-Gilbert resolution kernels (also referred to as a resolution matrix) by matrix operation. However, not all resolution matrices can provide resolution length information, and the computation of resolution matrix is often a difficult problem for very large inverse problems. A new class of resolution matrices, called the statistical resolution matrices (An, 2012, GJI), can be directly determined via a simple one-parameter nonlinear inversion performed based on limited pairs of random synthetic models and their inverse solutions. The total procedure were restricted to forward/inversion processes used in the real inverse problem and were independent of the degree of inverse skill used in the solution inversion. Spatial resolution lengths can be directly given during the inversion. Tests on 1D/2D/3D model inversion demonstrated that this simple method can be at least valid for a general linear inverse problem.

Development of a spatially resolving x-ray crystal spectrometer for measurement of ion-temperature (T(i)) and rotation-velocity (v) profiles in ITER.

PubMed

Hill, K W; Bitter, M; Delgado-Aparicio, L; Johnson, D; Feder, R; Beiersdorfer, P; Dunn, J; Morris, K; Wang, E; Reinke, M; Podpaly, Y; Rice, J E; Barnsley, R; O'Mullane, M; Lee, S G

2010-10-01

Imaging x-ray crystal spectrometer (XCS) arrays are being developed as a US-ITER activity for Doppler measurement of T(i) and v profiles of impurities (W, Kr, and Fe) with ∼7 cm (a/30) and 10-100 ms resolution in ITER. The imaging XCS, modeled after a prototype instrument on Alcator C-Mod, uses a spherically bent crystal and 2D x-ray detectors to achieve high spectral resolving power (E/dE>6000) horizontally and spatial imaging vertically. Two arrays will measure T(i) and both poloidal and toroidal rotation velocity profiles. The measurement of many spatial chords permits tomographic inversion for the inference of local parameters. The instrument design, predictions of performance, and results from C-Mod are presented.

Meso-scale defect evaluation of selective laser melting using spatially resolved acoustic spectroscopy

PubMed Central

Hirsch, M.; Catchpole-Smith, S.; Patel, R.; Marrow, P.; Li, Wenqi; Tuck, C.; Sharples, S. D.

2017-01-01

Developments in additive manufacturing technology are serving to expand the potential applications. Critical developments are required in the supporting areas of measurement and in process inspection to achieve this. CM247LC is a nickel superalloy that is of interest for use in aerospace and civil power plants. However, it is difficult to process via selective laser melting (SLM) as it suffers from cracking during rapid cooling and solidification. This limits the viability of CM247LC parts created using SLM. To quantify part integrity, spatially resolved acoustic spectroscopy (SRAS) has been identified as a viable non-destructive evaluation technique. In this study, a combination of optical microscopy and SRAS was used to identify and classify the surface defects present in SLM-produced parts. By analysing the datasets and scan trajectories, it is possible to correlate morphological information with process parameters. Image processing was used to quantify porosity and cracking for bulk density measurement. Analysis of surface acoustic wave data showed that an error in manufacture in the form of an overscan occurred. Comparing areas affected by overscan with a bulk material, a change in defect density from 1.17% in the bulk material to 5.32% in the overscan regions was observed, highlighting the need to reduce overscan areas in manufacture. PMID:28989306

Meso-scale defect evaluation of selective laser melting using spatially resolved acoustic spectroscopy.

PubMed

Hirsch, M; Catchpole-Smith, S; Patel, R; Marrow, P; Li, Wenqi; Tuck, C; Sharples, S D; Clare, A T

2017-09-01

Developments in additive manufacturing technology are serving to expand the potential applications. Critical developments are required in the supporting areas of measurement and in process inspection to achieve this. CM247LC is a nickel superalloy that is of interest for use in aerospace and civil power plants. However, it is difficult to process via selective laser melting (SLM) as it suffers from cracking during rapid cooling and solidification. This limits the viability of CM247LC parts created using SLM. To quantify part integrity, spatially resolved acoustic spectroscopy (SRAS) has been identified as a viable non-destructive evaluation technique. In this study, a combination of optical microscopy and SRAS was used to identify and classify the surface defects present in SLM-produced parts. By analysing the datasets and scan trajectories, it is possible to correlate morphological information with process parameters. Image processing was used to quantify porosity and cracking for bulk density measurement. Analysis of surface acoustic wave data showed that an error in manufacture in the form of an overscan occurred. Comparing areas affected by overscan with a bulk material, a change in defect density from 1.17% in the bulk material to 5.32% in the overscan regions was observed, highlighting the need to reduce overscan areas in manufacture.

Spatially resolved spectroscopy of WR ring nebulae. I - NGC 2359 and RCW 78

NASA Astrophysics Data System (ADS)

Esteban, C.; Vilchez, J. M.; Manchado, A.; Edmunds, M. G.

1990-01-01

We report detailed spatially resolved spectroscopy of the WR nebulae NGC 2359 and RCW 78 surrounding the WN 5 HD 56925 and WN 8 HD 117688 stars. The aim of this work has been to study possible abundance inhomogeneities and the ionization structure of the nebulae, as well as to derive reliable values for the degree of self-enrichment. In NGC 2359 the derived ratio O2+/O+ shows localized variations resulting from the compression of the gas in the filaments with respect to the shell gas. Effective temperatures for the central stars of both nebulae have been estimated on the basis of the ionization structure; the values found appear appropriate for their spectral types. Abundances of O/H, NIH, Ne/H, and He/H have been determined in twelve different positions in NGC 2359, covering its different morphological zones. No significant differences in the N/H and O/H abundances across the nebula have been found, although He/H shows marginal evidence for localized enhancements. In the case of RCW 78 the derived value of O/H is roughly solar, but He/H and NIH may be slightly overabundant. The results suggest that the amount of chemical self-enrichment of these WR nebulae is, at most, small.

Spatially resolved, diffuse reflectance imaging for subsurface pattern visualization toward development of a lensless imaging platform: phantom experiments

NASA Astrophysics Data System (ADS)

Schelkanova, Irina; Pandya, Aditya; Saiko, Guennadi; Nacy, Lidia; Babar, Hannan; Shah, Duoaud; Lilge, Lothar; Douplik, Alexandre

2016-01-01

A portable, spatially resolved, diffuse reflectance lensless imaging technique based on the charge-coupled device or complementary metal-oxide semiconductor sensor directly coupled to the fiber optic bundle is proposed for visualization of subsurface structures such as superficial microvasculature in the epithelium. We discuss an experimental method for emulating a lensless imaging setup via raster scanning a single fiber-optic cable over a microfluidic phantom containing periodic hemoglobin absorption contrast. To evaluate the ability of the technique to recover information about the subsurface linear structures, scattering layers formed of the Sylgard® 184 Silicone Elastomer and titanium dioxide were placed atop the microfluidic phantom. Thickness of the layers ranged from 0.2 to 0.7 mm, and the values of the reduced scattering coefficient (μs‧) were between 0.85 and 4.25 mm-1. The results demonstrate that fiber-optic, lensless platform can be used for two-dimensional imaging of absorbing inclusions in diffuse reflectance mode. In these experiments, it was shown that diffuse reflectance imaging can provide sufficient spatial sampling of the phantom for differentiation of 30 μm structural features of the embedded absorbing pattern inside the scattering media.

Quantitative characterization of the regressive ecological succession by fractal analysis of plant spatial patterns

USGS Publications Warehouse

Alados, C.L.; Pueyo, Y.; Giner, M.L.; Navarro, T.; Escos, J.; Barroso, F.; Cabezudo, B.; Emlen, J.M.

2003-01-01

We studied the effect of grazing on the degree of regression of successional vegetation dynamic in a semi-arid Mediterranean matorral. We quantified the spatial distribution patterns of the vegetation by fractal analyses, using the fractal information dimension and spatial autocorrelation measured by detrended fluctuation analyses (DFA). It is the first time that fractal analysis of plant spatial patterns has been used to characterize the regressive ecological succession. Plant spatial patterns were compared over a long-term grazing gradient (low, medium and heavy grazing pressure) and on ungrazed sites for two different plant communities: A middle dense matorral of Chamaerops and Periploca at Sabinar-Romeral and a middle dense matorral of Chamaerops, Rhamnus and Ulex at Requena-Montano. The two communities differed also in the microclimatic characteristics (sea oriented at the Sabinar-Romeral site and inland oriented at the Requena-Montano site). The information fractal dimension increased as we moved from a middle dense matorral to discontinuous and scattered matorral and, finally to the late regressive succession, at Stipa steppe stage. At this stage a drastic change in the fractal dimension revealed a change in the vegetation structure, accurately indicating end successional vegetation stages. Long-term correlation analysis (DFA) revealed that an increase in grazing pressure leads to unpredictability (randomness) in species distributions, a reduction in diversity, and an increase in cover of the regressive successional species, e.g. Stipa tenacissima L. These comparisons provide a quantitative characterization of the successional dynamic of plant spatial patterns in response to grazing perturbation gradient. ?? 2002 Elsevier Science B.V. All rights reserved.

Spatially resolved vertical vorticity in solar supergranulation using helioseismology and local correlation tracking

NASA Astrophysics Data System (ADS)

Langfellner, J.; Gizon, L.; Birch, A. C.

2015-09-01

Flow vorticity is a fundamental property of turbulent convection in rotating systems. Solar supergranules exhibit a preferred sense of rotation, which depends on the hemisphere. This is due to the Coriolis force acting on the diverging horizontal flows. We aim to spatially resolve the vertical flow vorticity of the average supergranule at different latitudes, both for outflow and inflow regions. To measure the vertical vorticity, we use two independent techniques: time-distance helioseismology (TD) and local correlation tracking of granules in intensity images (LCT) using data from the Helioseismic and Magnetic Imager (HMI) on board the Solar Dynamics Observatory (SDO). Both maps are corrected for center-to-limb systematic errors. We find that 8 h TD and LCT maps of vertical vorticity are highly correlated at large spatial scales. Associated with the average supergranule outflow, we find tangential (vortical) flows that reach about 10 m s-1 in the clockwise direction at 40° latitude. In average inflow regions, the tangential flow reaches the same magnitude, but in the anticlockwise direction. These tangential velocities are much smaller than the radial (diverging) flow component (300 m s-1 for the average outflow and 200 m s-1 for the average inflow). The results for TD and LCT as measured from HMI are in excellent agreement for latitudes between -60° and 60°. From HMI LCT, we measure the vorticity peak of the average supergranule to have a full width at half maximum of about 13 Mm for outflows and 8 Mm for inflows. This is larger than the spatial resolution of the LCT measurements (about 3 Mm). On the other hand, the vorticity peak in outflows is about half the value measured at inflows (e.g., 4 × 10-6 s-1 clockwise compared to 8 × 10-6 s-1 anticlockwise at 40° latitude). Results from the Michelson Doppler Imager (MDI) on board the Solar and Heliospheric Observatory (SOHO) obtained in 2010 are biased compared to the HMI/SDO results for the same period

Tempo-spatially resolved cellular dynamics of human immunodeficiency virus transacting activator of transcription (Tat) peptide-modified nanocargos in living cells

NASA Astrophysics Data System (ADS)

Wei, Lin; Yang, Qiaoyu; Xiao, Lehui

2014-08-01

Understanding the cellular uptake mechanism and intracellular fate of nanocarriers in living cells is of great importance for the rational design of efficient drug delivery cargos as well as the development of robust biomedical diagnostic probes. In present study, with a dual wavelength view darkfield microscope (DWVD), the tempo-spatially resolved dynamics of Tat peptide-functionalized gold nanoparticles (TGNPs, with size similar to viruses) in living HeLa cells were extensively explored. It was found that energy-dependent endocytosis (both clathrin- and caveolae-mediated processes were involved) was the prevailing pathway for the cellular uptake of TGNPs. The time-correlated dynamic spatial distribution information revealed that TGNPs could not actively target the cell nuclei, which is contrary to previous observations based on fixed cell results. More importantly, the inheritance of TGNPs to the daughter cells through mitosis was found to be the major route to metabolize TGNPs by HeLa cells. These understandings on the cellular uptake mechanism and intracellular fate of nanocargos in living cells would provide deep insight on how to improve and controllably manipulate their translocation efficiency for targeted drug delivery.Understanding the cellular uptake mechanism and intracellular fate of nanocarriers in living cells is of great importance for the rational design of efficient drug delivery cargos as well as the development of robust biomedical diagnostic probes. In present study, with a dual wavelength view darkfield microscope (DWVD), the tempo-spatially resolved dynamics of Tat peptide-functionalized gold nanoparticles (TGNPs, with size similar to viruses) in living HeLa cells were extensively explored. It was found that energy-dependent endocytosis (both clathrin- and caveolae-mediated processes were involved) was the prevailing pathway for the cellular uptake of TGNPs. The time-correlated dynamic spatial distribution information revealed that TGNPs

Spatially resolved synchrotron radiation induced X-ray fluorescence analyses of rare Rembrandt silverpoint drawings

NASA Astrophysics Data System (ADS)

Reiche, I.; Radtke, M.; Berger, A.; Görner, W.; Merchel, S.; Riesemeier, H.; Bevers, H.

2006-05-01

New analyses of a series of very rare silverpoint drawings that were executed by Rembrandt Harmensz. van Rijn (1606 1669) which are kept today in the Kupferstichkabinett (Museum of Prints and Drawings) of the State Museums of Berlin are reported here. Analysis of these drawings requires particular attention because the study has to be fully non-destructive and extremely sensitive. The metal alloy on the paper does not exceed some hundreds of μg/cm2. Therefore, synchrotron radiation induced X-ray fluorescence (SR-XRF) is together with external micro-proton-induced X-ray emission the only well-suited method for the analyses of metalpoint drawings. In some primary work, about 25 German and Flemish metalpoint drawings were investigated using spatially resolved SR-XRF analysis at the BAMline at BESSY. This study enlarges the existing French German database of metalpoint drawings dating from the 15th and 16th centuries, as these Rembrandt drawings originate from the 17th century where this graphical technique was even rarer and already obsolete. It also illustrates how SR-XRF analysis can reinforce art historical assumptions on the dating of drawings and their connection.

Quantitative fluorescence imaging of protein diffusion and interaction in living cells.

PubMed

Capoulade, Jérémie; Wachsmuth, Malte; Hufnagel, Lars; Knop, Michael

2011-08-07

Diffusion processes and local dynamic equilibria inside cells lead to nonuniform spatial distributions of molecules, which are essential for processes such as nuclear organization and signaling in cell division, differentiation and migration. To understand these mechanisms, spatially resolved quantitative measurements of protein abundance, mobilities and interactions are needed, but current methods have limited capabilities to study dynamic parameters. Here we describe a microscope based on light-sheet illumination that allows massively parallel fluorescence correlation spectroscopy (FCS) measurements and use it to visualize the diffusion and interactions of proteins in mammalian cells and in isolated fly tissue. Imaging the mobility of heterochromatin protein HP1α (ref. 4) in cell nuclei we could provide high-resolution diffusion maps that reveal euchromatin areas with heterochromatin-like HP1α-chromatin interactions. We expect that FCS imaging will become a useful method for the precise characterization of cellular reaction-diffusion processes.

High-resolution, time-resolved MRA provides superior definition of lower-extremity arterial segments compared to 2D time-of-flight imaging.

PubMed

Thornton, F J; Du, J; Suleiman, S A; Dieter, R; Tefera, G; Pillai, K R; Korosec, F R; Mistretta, C A; Grist, T M

2006-08-01

To evaluate a novel time-resolved contrast-enhanced (CE) projection reconstruction (PR) magnetic resonance angiography (MRA) method for identifying potential bypass graft target vessels in patients with Class II-IV peripheral vascular disease. Twenty patients (M:F = 15:5, mean age = 58 years, range = 48-83 years), were recruited from routine MRA referrals. All imaging was performed on a 1.5 T MRI system with fast gradients (Signa LX; GE Healthcare, Waukesha, WI). Images were acquired with a novel technique that combined undersampled PR with a time-resolved acquisition to yield an MRA method with high temporal and spatial resolution. The method is called PR hyper time-resolved imaging of contrast kinetics (PR-hyperTRICKS). Quantitative and qualitative analyses were used to compare two-dimensional (2D) time-of-flight (TOF) and PR-hyperTRICKS in 13 arterial segments per lower extremity. Statistical analysis was performed with the Wilcoxon signed-rank test. Fifteen percent (77/517) of the vessels were scored as missing or nondiagnostic with 2D TOF, but were scored as diagnostic with PR-hyperTRICKS. Image quality was superior with PR-hyperTRICKS vs. 2D TOF (on a four-point scale, mean rank = 3.3 +/- 1.2 vs. 2.9 +/- 1.2, P < 0.0001). PR-hyperTRICKS produced images with high contrast-to-noise ratios (CNR) and high spatial and temporal resolution. 2D TOF images were of inferior quality due to moderate spatial resolution, inferior CNR, greater flow-related artifacts, and absence of temporal resolution. PR-hyperTRICKS provides superior preoperative assessment of lower limb ischemia compared to 2D TOF.

Spatially resolved frequency domain phosphorescence lifetime-based oxygen sensing for photodynamic therapy

NASA Astrophysics Data System (ADS)

Lai, Benjamin; Gurari, Mark; Wee, Wallace; Lilge, Lothar

2008-06-01

Photodynamic Therapy (PDT) is a minimally invasive treatment that uses a photosensitive drug into convert triplet state oxygen (3O2) to singlet oxygen (1O2) to destroy malignant tissue. A fiber-optic system based on frequency domain detection of phosphorescence quenching by 3O2 is described which optically measures the distribution of 3O2 in the treatment volume during PDT to permit adjustments of treatment parameters to improve outcome. A specially designed fiber optic probe containing phosphorescent sensors embedded along its length permit spatially resolved measurements. Each sensor is composed of a phosphorescent metalloporphyrin compound that emits a characteristic spectrum. Four candidate sensors with high absorption at the excitation wavelength of 405nm and emission in the 650nm to 700nm region are considered. The dependence of phosphorescence lifetime (τ) on 3O2 concentration is described by the linearized Stern-Volmer relationship as being inversely proportional. Determination of τ, and hence 3O2 concentration, is accomplished in the frequency domain by means of phase-modulation detection of the phosphorescence signal due to an amplitude modulated excitation. The τ's of each sensor are recovered by performing global non-linear least squares fit on the measured phase and modulation index over a range of frequencies and wavelengths. With the τ of each sensor known, the oxygen concentration at each sensor's location can be determined with the Stern-Volmer relationship.

The PyCASSO database: spatially resolved stellar population properties for CALIFA galaxies

NASA Astrophysics Data System (ADS)

de Amorim, A. L.; García-Benito, R.; Cid Fernandes, R.; Cortijo-Ferrero, C.; González Delgado, R. M.; Lacerda, E. A. D.; López Fernández, R.; Pérez, E.; Vale Asari, N.

2017-11-01

The Calar Alto Legacy Integral Field Area (CALIFA) survey, a pioneer in integral field spectroscopy legacy projects, has fostered many studies exploring the information encoded on the spatially resolved data on gaseous and stellar features in the optical range of galaxies. We describe a value-added catalogue of stellar population properties for CALIFA galaxies analysed with the spectral synthesis code starlight and processed with the pycasso platform. Our public database (http://pycasso.ufsc.br/, mirror at http://pycasso.iaa.es/) comprises 445 galaxies from the CALIFA Data Release 3 with COMBO data. The catalogue provides maps for the stellar mass surface density, mean stellar ages and metallicities, stellar dust attenuation, star formation rates, and kinematics. Example applications both for individual galaxies and for statistical studies are presented to illustrate the power of this data set. We revisit and update a few of our own results on mass density radial profiles and on the local mass-metallicity relation. We also show how to employ the catalogue for new investigations, and show a pseudo Schmidt-Kennicutt relation entirely made with information extracted from the stellar continuum. Combinations to other databases are also illustrated. Among other results, we find a very good agreement between star formation rate surface densities derived from the stellar continuum and the H α emission. This public catalogue joins the scientific community's effort towards transparency and reproducibility, and will be useful for researchers focusing on (or complementing their studies with) stellar properties of CALIFA galaxies.

Self-Assembled Tb3+ Complex Probe for Quantitative Analysis of ATP during Its Enzymatic Hydrolysis via Time-Resolved Luminescence in Vitro and in Vivo.

PubMed

Jung, Sung Ho; Kim, Ka Young; Lee, Ji Ha; Moon, Cheol Joo; Han, Noh Soo; Park, Su-Jin; Kang, Dongmin; Song, Jae Kyu; Lee, Shim Sung; Choi, Myong Yong; Jaworski, Justyn; Jung, Jong Hwa

2017-01-11

To more accurately assess the pathways of biological systems, a probe is needed that may respond selectively to adenosine triphosphate (ATP) for both in vitro and in vivo detection modes. We have developed a luminescence probe that can provide real-time information on the extent of ATP, ADP, and AMP by virtue of the luminescence and luminescence lifetime observed from a supramolecular polymer based on a C 3 symmetrical terpyridine complex with Tb 3+ (S1-Tb). The probe shows remarkable selective luminescence enhancement in the presence of ATP compared to other phosphate-displaying nucleotides including adenosine diphosphate (ADP), adenosine monophosphate (AMP), guanosine triphosphate (GTP), thymidine triphosphate (TTP), H 2 PO 4 - (Pi), and pyrophosphate (PPi). In addition, the time-resolved luminescence lifetime and luminescence spectrum of S1-Tb could facilitate the quantitative measurement of the exact amount of ATP and similarly ADP and AMP within living cells. The time-resolved luminescence lifetime of S1-Tb could also be used to quantitatively monitor the amount of ATP, ADP, and AMP in vitro following the enzymatic hydrolysis of ATP. The long luminescence lifetime, which was observed into the millisecond range, makes this S1-Tb-based probe particularly attractive for monitoring biological ATP levels in vivo, because any short lifetime background fluorescence arising from the complex molecular environment may be easily eliminated.

Spatially resolved bimodal spectroscopy for classification/evaluation of mouse skin inflammatory and pre-cancerous stages

NASA Astrophysics Data System (ADS)

Díaz-Ayil, Gilberto; Amouroux, Marine; Clanché, Fabien; Granjon, Yves; Blondel, Walter C. P. M.

2009-07-01

Spatially-resolved bimodal spectroscopy (multiple AutoFluorescence AF excitation and Diffuse Reflectance DR), was used in vivo to discriminate various healthy and precancerous skin stages in a pre-clinical model (UV-irradiated mouse): Compensatory Hyperplasia CH, Atypical Hyperplasia AH and Dysplasia D. A specific data preprocessing scheme was applied to intensity spectra (filtering, spectral correction and intensity normalization), and several sets of spectral characteristics were automatically extracted and selected based on their discrimination power, statistically tested for every pair-wise comparison of histological classes. Data reduction with Principal Components Analysis (PCA) was performed and 3 classification methods were implemented (k-NN, LDA and SVM), in order to compare diagnostic performance of each method. Diagnostic performance was studied and assessed in terms of Sensibility (Se) and Specificity (Sp) as a function of the selected features, of the combinations of 3 different inter-fibres distances and of the numbers of principal components, such that: Se and Sp ~ 100% when discriminating CH vs. others; Sp ~ 100% and Se > 95% when discriminating Healthy vs. AH or D; Sp ~ 74% and Se ~ 63% for AH vs. D.

Experimental observation of spatially resolved photo-luminescence intensity distribution in dual mode upconverting nanorod bundles

PubMed Central

Kumar, Pawan; Singh, Satbir; Singh, V. N.; Singh, Nidhi; Gupta, R. K.; Gupta, Bipin Kumar

2017-01-01

A novel method for demonstration of photoluminescence intensity distribution in upconverting nanorod bundles using confocal microscopy is reported. Herein, a strategy for the synthesis of highly luminescent dual mode upconverting/downshift Y1.94O3:Ho3+0.02/Yb3+0.04 nanorod bundles by a facile hydrothermal route has been introduced. These luminescent nanorod bundles exhibit strong green emission at 549 nm upon excitations at 449 nm and 980 nm with quantum efficiencies of ~6.3% and ~1.1%, respectively. The TEM/HRTEM results confirm that these bundles are composed of several individual nanorods with diameter of ~100 nm and length in the range of 1–3 μm. Furthermore, two dimensional spatially resolved photoluminescence intensity distribution study has been carried out using confocal photoluminescence microscope throughout the nanorod bundles. This study provides a new direction for the potential use of such emerging dual mode nanorod bundles as photon sources for next generation flat panel optical display devices, bio-medical applications, luminescent security ink and enhanced energy harvesting in photovoltaic applications. PMID:28211891

The Balloon Experimental Twin Telescope for Infrared Interferometry (BETTII): Spatially Resolved Spectroscopy in the Far-Infrared

NASA Technical Reports Server (NTRS)

Rinehart, Stephen

2009-01-01

Astronomical studies at infrared wavelengths have dramatically improved our understanding of the universe, and observations with Spitzer, the upcoming Herschel mission, and SOFIA will continue to provide exciting new discoveries. The relatively low angular resolution of these missions, however, is insufficient to resolve the physical scale on which mid-to far-infrared emission arises, resulting in source and structure ambiguities that limit our ability to answer key science questions. Interferometry enables high angular resolution at these wavelengths - a powerful tool for scientific discovery. We will build the Balloon Experimental Twin Telescope for Infrared Interferometry (BETTII), an eight-meter baseline Michelson stellar interferometer to fly on a high-altitude balloon. BETTII's spectral-spatial capability, provided by an instrument using double-Fourier techniques, will address key questions about the nature of disks in young star clusters and active galactic nuclei and the envelopes of evolved stars. BETTII will also lay the technological groundwork for future space interferometers and for suborbital programs optimized for studying extrasolar planets.

Compact Starburst Galaxies with Fast Outflows: Spatially Resolved Stellar Mass Profiles

NASA Astrophysics Data System (ADS)

Gottlieb, Sophia; Diamond-Stanic, Aleksandar; Lipscomb, Charles; Ohene, Senyo; Rines, Josh; Moustakas, John; Sell, Paul; Tremonti, Christy; Coil, Alison; Rudnick, Gregory; Hickox, Ryan C.; Geach, James; Kepley, Amanda

2018-01-01

Powerful galactic winds driven by stellar feedback and black hole accretion are thought to play an important role in regulating star formation in galaxies. In particular, strong stellar feedback from supernovae, stellar winds, radiation pressure, and cosmic rays is required by simulations of star-forming galaxies to prevent the vast majority of baryons from cooling and collapsing to form stars. However, it remains unclear whether these stellar processes play a significant role in expelling gas and shutting down star formation in massive progenitors of quiescent galaxies. What are the limits of stellar feedback? We present multi-band photometry with HST/WFC3 (F475W, F814W, F160W) for a dozen compact starburst galaxies at z~0.6 with half-light radii that suggest incredibly large central escape velocities. These massive galaxies are driving fast (>1000 km/s) outflows that have been previously attributed to stellar feedback associated with the compact (r~100 pc) starburst. But how compact is the stellar mass? In the context of the stellar feedback hypothesis, it is unclear whether these fast outflows are being driven at velocities comparable to the escape velocity of an incredibly dense stellar system (as predicted by some models of radiation-pressure winds) or at velocities that exceed the central escape velocity by large factor. Our spatially resolved measurements with HST show that the stellar mass is more extended than the light, and this requires that the physical mechanism responsible for driving the winds must be able to launch gas at velocities that are factors of 5-10 beyond the central escape velocity.

Comparison of two spatially-resolved fossil fuel CO2 emissions inventories at the urban scale in four US cities

NASA Astrophysics Data System (ADS)

Liang, J.; Gurney, K. R.; O'Keeffe, D.; Patarasuk, R.; Hutchins, M.; Rao, P.

2017-12-01

Spatially-resolved fossil fuel CO2 (FFCO2) emissions are used not only in complex atmospheric modeling systems as prior scenarios to simulate concentrations of CO2 in the atmosphere, but to improve understanding of relationships with socioeconomic factors in support of sustainability policymaking. We present a comparison of ODIAC, a top-down global gridded FFCO2 emissions dataset, and Hesita, a bottom-up FFCO2 emissions dataset, in four US cities, including Los Angles, Indianapolis, Salt Lake City and Baltimore City. ODIAC was developed by downscaling national total emissions to 1km-by-1km grid cells using satellite nightlight imagery as proxy. Hesita was built from the ground up by allocating sector-specific county-level emissions to urban-level spatial surrogates including facility locations, road maps, building footprints/parcels, railroad maps and shipping lanes. The differences in methodology and data sources could lead to large discrepancies in FFCO2 estimates at the urban scale, and these discrepancies need to be taken into account in conducting atmospheric modeling or socioeconomic analysis. This comparison work is aimed at quantifying the statistical and spatial difference between the two FFCO2 inventories. An analysis of the difference in total emissions, spatial distribution and statistical distribution resulted in the following findings: (1) ODIAC agrees well with Hestia in total FFCO2 emissions estimates across the four cities with a difference from 3%-20%; (2) Small-scale areal and linear spatial features such as roads and buildings are either entirely missing or not very well represented in ODIAC, since nightlight imagery might not be able to capture these information. This might further lead to underestimated on-road FFCO2 emissions in ODIAC; (3) The statistical distribution of ODIAC is more concentrated around the mean with much less samples in the lower range. These phenomena could result from the nightlight halo and saturation effects; (4) The

Spatially and chemically resolved source apportionment analysis: Case study of high particulate matter event

NASA Astrophysics Data System (ADS)

Kim, Byeong-Uk; Bae, Changhan; Kim, Hyun Cheol; Kim, Eunhye; Kim, Soontae

2017-08-01

This article presents the results of a detailed source apportionment study of the high particulate matter (PM) event in the Seoul Metropolitan Area (SMA), South Korea, during late February 2014. Using the Comprehensive Air Quality Model with Extensions with its Particulate Source Apportionment Technology (CAMx-PSAT), we defined 10 source regions, including five in China, for spatially and chemically resolved analyses. During the event, the spatially averaged PM10 concentration at all PM10 monitors in the SMA was 129 μg/m3, while the PM10 and PM2.5 concentrations at the BulGwang Supersite were 143 μg/m3 and 123 μg/m3, respectively. CAMx-PSAT showed reasonably good PM model performance in both China and the SMA. For February 23-27, CAMx-PSAT estimated that Chinese contributions to the SMA PM10 and PM2.5 were 84.3 μg/m3 and 80.0 μg/m3, respectively, or 64% and 70% of the respective totals, while South Korea's respective domestic contributions were 36.5 μg/m3 and 23.3 μg/m3. We observed that the spatiotemporal pattern of PM constituent concentrations and contributions did not necessarily follow that of total PM10 and PM2.5 concentrations. For example, Beijing-Tianjin-Hebei produced high nitrate concentrations, but the two most-contributing regions to PM in the SMA were the Near Beijing area and South Korea. In addition, we noticed that the relative contributions from each region changed over time. We found that most ammonium mass that neutralized Chinese sulfate mass in the SMA came from South Korean sources, indicating that secondary inorganic aerosol in the SMA, especially ammonium sulfates, during this event resulted from different major precursors originating from different regions.

Probing microscopic material properties inside simulated membranes through spatially resolved three-dimensional local pressure fields and surface tensions

PubMed Central

Kasson, Peter M.; Hess, Berk; Lindahl, Erik

2013-01-01

Cellular lipid membranes are spatially inhomogeneous soft materials. Materials properties such as pressure and surface tension thus show important microscopic-scale variation that is critical to many biological functions. We present a means to calculate pressure and surface tension in a 3D-resolved manner within molecular-dynamics simulations and show how such measurements can yield important insight. We also present the first corrections to local virial and pressure fields to account for the constraints typically used in lipid simulations that otherwise cause problems in highly oriented systems such as bilayers. Based on simulations of an asymmetric bacterial ion channel in a POPC bilayer, we demonstrate how 3D-resolved pressure can probe for both short-range and long-range effects from the protein on the membrane environment. We also show how surface tension is a sensitive metric for inter-leaflet equilibrium and can be used to detect even subtle imbalances between bilayer leaflets in a membrane-protein simulation. Since surface tension is known to modulate the function of many proteins, this effect is an important consideration for predictions of ion channel function. We outline a strategy by which our local pressure measurements, which we make available within a version of the GROMACS simulation package, may be used to design optimally equilibrated membrane-protein simulations. PMID:23318532

Quantitative evaluation of the risk induced by dominant geomorphological processes on different land uses, based on GIS spatial analysis models

NASA Astrophysics Data System (ADS)

Ştefan, Bilaşco; Sanda, Roşca; Ioan, Fodorean; Iuliu, Vescan; Sorin, Filip; Dănuţ, Petrea

2017-12-01

Maramureş Land is mostly characterized by agricultural and forestry land use due to its specific configuration of topography and its specific pedoclimatic conditions. Taking into consideration the trend of the last century from the perspective of land management, a decrease in the surface of agricultural lands to the advantage of built-up and grass lands, as well as an accelerated decrease in the forest cover due to uncontrolled and irrational forest exploitation, has become obvious. The field analysis performed on the territory of Maramureş Land has highlighted a high frequency of two geomorphologic processes — landslides and soil erosion — which have a major negative impact on land use due to their rate of occurrence. The main aim of the present study is the GIS modeling of the two geomorphologic processes, determining a state of vulnerability (the USLE model for soil erosion and a quantitative model based on the morphometric characteristics of the territory, derived from the HG. 447/2003) and their integration in a complex model of cumulated vulnerability identification. The modeling of the risk exposure was performed using a quantitative approach based on models and equations of spatial analysis, which were developed with modeled raster data structures and primary vector data, through a matrix highlighting the correspondence between vulnerability and land use classes. The quantitative analysis of the risk was performed by taking into consideration the exposure classes as modeled databases and the land price as a primary alphanumeric database using spatial analysis techniques for each class by means of the attribute table. The spatial results highlight the territories with a high risk to present geomorphologic processes that have a high degree of occurrence and represent a useful tool in the process of spatial planning.

Quantitative evaluation of the risk induced by dominant geomorphological processes on different land uses, based on GIS spatial analysis models

NASA Astrophysics Data System (ADS)

Ştefan, Bilaşco; Sanda, Roşca; Ioan, Fodorean; Iuliu, Vescan; Sorin, Filip; Dănuţ, Petrea

2018-06-01

Maramureş Land is mostly characterized by agricultural and forestry land use due to its specific configuration of topography and its specific pedoclimatic conditions. Taking into consideration the trend of the last century from the perspective of land management, a decrease in the surface of agricultural lands to the advantage of built-up and grass lands, as well as an accelerated decrease in the forest cover due to uncontrolled and irrational forest exploitation, has become obvious. The field analysis performed on the territory of Maramureş Land has highlighted a high frequency of two geomorphologic processes — landslides and soil erosion — which have a major negative impact on land use due to their rate of occurrence. The main aim of the present study is the GIS modeling of the two geomorphologic processes, determining a state of vulnerability (the USLE model for soil erosion and a quantitative model based on the morphometric characteristics of the territory, derived from the HG. 447/2003) and their integration in a complex model of cumulated vulnerability identification. The modeling of the risk exposure was performed using a quantitative approach based on models and equations of spatial analysis, which were developed with modeled raster data structures and primary vector data, through a matrix highlighting the correspondence between vulnerability and land use classes. The quantitative analysis of the risk was performed by taking into consideration the exposure classes as modeled databases and the land price as a primary alphanumeric database using spatial analysis techniques for each class by means of the attribute table. The spatial results highlight the territories with a high risk to present geomorphologic processes that have a high degree of occurrence and represent a useful tool in the process of spatial planning.

Stochastic optical reconstruction microscopy-based relative localization analysis (STORM-RLA) for quantitative nanoscale assessment of spatial protein organization.

PubMed

Veeraraghavan, Rengasayee; Gourdie, Robert G

2016-11-07

The spatial association between proteins is crucial to understanding how they function in biological systems. Colocalization analysis of fluorescence microscopy images is widely used to assess this. However, colocalization analysis performed on two-dimensional images with diffraction-limited resolution merely indicates that the proteins are within 200-300 nm of each other in the xy-plane and within 500-700 nm of each other along the z-axis. Here we demonstrate a novel three-dimensional quantitative analysis applicable to single-molecule positional data: stochastic optical reconstruction microscopy-based relative localization analysis (STORM-RLA). This method offers significant advantages: 1) STORM imaging affords 20-nm resolution in the xy-plane and <50 nm along the z-axis; 2) STORM-RLA provides a quantitative assessment of the frequency and degree of overlap between clusters of colabeled proteins; and 3) STORM-RLA also calculates the precise distances between both overlapping and nonoverlapping clusters in three dimensions. Thus STORM-RLA represents a significant advance in the high-throughput quantitative assessment of the spatial organization of proteins. © 2016 Veeraraghavan and Gourdie. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

Using broadband spatially resolved NIRS to assess muscle oxygenation during altered running protocols

NASA Astrophysics Data System (ADS)

Koukourakis, Georg; Vafiadou, Maria; Steimers, André; Geraskin, Dmitri; Neary, Patrick; Kohl-Bareis, Matthias

2009-07-01

We used spatially resolved near-infrared spectroscopy (SRS-NIRS) to assess calf and thigh muscle oxygenation during running on a motor-driven treadmill. Two protocols were used: An incremental speed protocol (velocity = 6 - 12 km/h, ▵v = 2 km/h) was performed in 3 minute stages, while a pacing paradigm modulated step frequency alternatively (2.3 Hz [SLow]; 3.3 Hz [SHigh]) during a constant velocity for 2 minutes each. A SRS-NIRS broadband system (600 - 1000 nm) was used to measure total haemoglobin concentration and oxygen saturation (SO2). An accelerometer was placed on the hip joints to measure limb acceleration through the experiment. The data showed that the calf (SO2 58 to 42%) desaturated to a significantly lower level than the thigh (61 to 54%). During the pacing protocol, SO2 was significantly different between the SLow vs. SHigh trials. Additionally, physiological data as measured by spirometry were different between the SLow vs. SHigh pacing trials (VO2 (2563+/- 586 vs. 2503 +/- 605 mL/min). Significant differences in VO2 at the same workload (speed) indicate alterations in mechanical efficiency. These data suggest that SRS broadband NIRS can be used to discern small changes in muscle oxygenation, making this device useful for metabolic exercise studies in addition to spirometry and movement monitoring by accelerometers.

Spatially-resolved velocities of thermally-produced spray droplets using a velocity-divided Abel inversion of photographed streaks

NASA Astrophysics Data System (ADS)

Kawaguchi, Y.; Kobayashi, N.; Yamagata, Y.; Miyazaki, F.; Yamasaki, M.; Muraoka, K.

2017-10-01

Droplet velocities of thermal spray are known to have profound effects on important coating qualities, such as adhesive strength, porosity, and hardness, for various applications. For obtaining the droplet velocities, therefore, the TOF (time-of-flight) technique has been widely used, which relies on observations of emitted radiation from the droplets, where all droplets along the line-of-sight contribute to signals. Because droplets at and near the flow axis mostly contribute coating layers, it has been hoped to get spatially resolved velocities. For this purpose, a velocity-divided Abel inversion was devised from CMOS photographic data. From this result, it has turned out that the central velocity is about 25% higher than that obtained from the TOF technique for the case studied (at the position 150 mm downstream of the plasma spray gun, where substrates for spray coatings are usually placed). Further implications of the obtained results are discussed.

Recent Advances in 3D Time-Resolved Contrast-Enhanced MR Angiography

PubMed Central

Riederer, Stephen J.; Haider, Clifton R.; Borisch, Eric A.; Weavers, Paul T.; Young, Phillip M.

2015-01-01

Contrast-enhanced MR angiography (CE-MRA) was first introduced for clinical studies approximately 20 years ago. Early work provided 3 to 4 mm spatial resolution with acquisition times in the 30 sec range. Since that time there has been continuing effort to provide improved spatial resolution with reduced acquisition time, allowing high resolution three-dimensional (3D) time-resolved studies. The purpose of this work is to describe how this has been accomplished. Specific technical enablers have been: improved gradients allowing reduced repetition times, improved k-space sampling and reconstruction methods, parallel acquisition particularly in two directions, and improved and higher count receiver coil arrays. These have collectively made high resolution time-resolved studies readily available for many anatomic regions. Depending on the application, approximate 1 mm isotropic resolution is now possible with frame times of several seconds. Clinical applications of time-resolved CE-MRA are briefly reviewed. PMID:26032598

A Spatially Resolved Study of Cold Dust, Molecular Gas, H II Regions, and Stars in the z = 2.12 Submillimeter Galaxy ALESS67.1

NASA Astrophysics Data System (ADS)

Chen, Chian-Chou; Hodge, J. A.; Smail, Ian; Swinbank, A. M.; Walter, Fabian; Simpson, J. M.; Calistro Rivera, Gabriela; Bertoldi, F.; Brandt, W. N.; Chapman, S. C.; da Cunha, Elisabete; Dannerbauer, H.; De Breuck, C.; Harrison, C. M.; Ivison, R. J.; Karim, A.; Knudsen, K. K.; Wardlow, J. L.; Weiß, A.; van der Werf, P. P.

2017-09-01

We present detailed studies of a z = 2.12 submillimeter galaxy, ALESS67.1, using sub-arcsecond resolution ALMA, adaptive optics-aided VLT/SINFONI, and Hubble Space Telescope (HST)/CANDELS data to investigate the kinematics and spatial distributions of dust emission (870 μm continuum), 12CO(J = 3–2), strong optical emission lines, and visible stars. Dynamical modeling of the optical emission lines suggests that ALESS67.1 is not a pure rotating disk but a merger, consistent with the apparent tidal features revealed in the HST imaging. Our sub-arcsecond resolution data set allows us to measure half-light radii for all the tracers, and we find a factor of 4–6 smaller sizes in dust continuum compared to all the other tracers, including 12CO; also, ultraviolet (UV) and Hα emission are significantly offset from the dust continuum. The spatial mismatch between the UV continuum and the cold dust and gas reservoir supports the explanation that geometrical effects are responsible for the offset of the dusty galaxy on the IRX–β diagram. Using a dynamical method we derive an {α }CO}=1.8+/- 1.0, consistent with other submillimeter galaxies (SMGs) that also have resolved CO and dust measurements. Assuming a single {α }CO} value we also derive resolved gas and star formation rate surface densities, and find that the core region of the galaxy (≲ 5 kpc) follows the trend of mergers on the Schmidt–Kennicutt relationship, whereas the outskirts (≳ 5 kpc) lie on the locus of normal star-forming galaxies, suggesting different star formation efficiencies within one galaxy. Our results caution against using single size or morphology for different tracers of the star formation activity and gas content of galaxies, and therefore argue the need to use spatially resolved, multi-wavelength observations to interpret the properties of SMGs, and perhaps even for z> 1 galaxies in general.

Spatially resolved mapping of electrical conductivity across individual domain (grain) boundaries in graphene.

PubMed

Clark, Kendal W; Zhang, X-G; Vlassiouk, Ivan V; He, Guowei; Feenstra, Randall M; Li, An-Ping

2013-09-24

All large-scale graphene films contain extended topological defects dividing graphene into domains or grains. Here, we spatially map electronic transport near specific domain and grain boundaries in both epitaxial graphene grown on SiC and CVD graphene on Cu subsequently transferred to a SiO2 substrate, with one-to-one correspondence to boundary structures. Boundaries coinciding with the substrate step on SiC exhibit a significant potential barrier for electron transport of epitaxial graphene due to the reduced charge transfer from the substrate near the step edge. Moreover, monolayer-bilayer boundaries exhibit a high resistance that can change depending on the height of substrate step coinciding at the boundary. In CVD graphene, the resistance of a grain boundary changes with the width of the disordered transition region between adjacent grains. A quantitative modeling of boundary resistance reveals the increased electron Fermi wave vector within the boundary region, possibly due to boundary induced charge density variation. Understanding how resistance change with domain (grain) boundary structure in graphene is a crucial first step for controlled engineering of defects in large-scale graphene films.

Nanoscale nuclear architecture for cancer diagnosis by spatial-domain low-coherence quantitative phase microscopy

NASA Astrophysics Data System (ADS)

Wang, Pin; Bista, Rajan K.; Khalbuss, Walid E.; Qiu, Wei; Staton, Kevin D.; Zhang, Lin; Brentnall, Teresa A.; Brand, Randall E.; Liu, Yang

2011-03-01

Alterations in nuclear architecture are the hallmark diagnostic characteristic of cancer cells. In this work, we show that the nuclear architectural characteristics quantified by spatial-domain low-coherence quantitative phase microscopy (SL-QPM), is more sensitive for the identification of cancer cells than conventional cytopathology. We demonstrated the importance of nuclear architectural characteristics in both an animal model of intestinal carcinogenesis - APC/Min mouse model and human cytology specimens with colorectal cancer by identifying cancer from cytologically noncancerous appearing cells. The determination of nanoscale nuclear architecture using this simple and practical optical instrument is a significant advance towards cancer diagnosis.

Quantifying Feedback from Narrow Line Region Outflows in Nearby Active Galaxies. I. Spatially Resolved Mass Outflow Rates for the Seyfert 2 Galaxy Markarian 573

NASA Astrophysics Data System (ADS)

Revalski, M.; Crenshaw, D. M.; Kraemer, S. B.; Fischer, T. C.; Schmitt, H. R.; Machuca, C.

2018-03-01

We present the first spatially resolved mass outflow rate measurements ({\\dot{M}}out}) of the optical emission line gas in the narrow line region (NLR) of a Seyfert 2 galaxy, Markarian 573. Using long slit spectra and [O III] imaging from the Hubble Space Telescope and Apache Point Observatory in conjunction with emission line diagnostics and Cloudy photoionization models, we find a peak outflow rate of {\\dot{M}}out}≈ 3.4 +/- 0.5 {M}ȯ {yr}}-1 at a distance of 210 pc from the central supermassive black hole (SMBH). The outflow extends to distances of 600 pc from the nucleus with a total mass and kinetic energy of M ≈ 2.2 × 106 M ⊙ and E ≈ 5.1 × 1054 erg, revealing the outflows to be more energetic than those in the lower luminosity Seyfert 1 galaxy NGC 4151. The peak outflow rate is an order of magnitude larger than the mass accretion and nuclear outflow rates, indicating local in situ acceleration of the circumnuclear NLR gas. We compare these results to global techniques that quantify an average outflow rate across the NLR, and find the latter are subject to larger uncertainties. These results indicate that spatially resolved observations are critical for probing AGN feedback on scales where circumnuclear star formation occurs.

Variability in Spatially and Temporally Resolved Emissions and Hydrocarbon Source Fingerprints for Oil and Gas Sources in Shale Gas Production Regions.

PubMed

Allen, David T; Cardoso-Saldaña, Felipe J; Kimura, Yosuke

2017-10-17

A gridded inventory for emissions of methane, ethane, propane, and butanes from oil and gas sources in the Barnett Shale production region has been developed. This inventory extends previous spatially resolved inventories of emissions by characterizing the overall variability in emission magnitudes and the composition of emissions at an hourly time resolution. The inventory is divided into continuous and intermittent emission sources. Sources are defined as continuous if hourly averaged emissions are greater than zero in every hour; otherwise, they are classified as intermittent. In the Barnett Shale, intermittent sources accounted for 14-30% of the mean emissions for methane and 10-34% for ethane, leading to spatial and temporal variability in the location of hourly emissions. The combined variability due to intermittent sources and variability in emission factors can lead to wide confidence intervals in the magnitude and composition of time and location-specific emission inventories; therefore, including temporal and spatial variability in emission inventories is important when reconciling inventories and observations. Comparisons of individual aircraft measurement flights conducted in the Barnett Shale region versus the estimated emission rates for each flight from the emission inventory indicate agreement within the expected variability of the emission inventory for all flights for methane and for all but one flight for ethane.

Quantitative assessment of neural outgrowth using spatial light interference microscopy

NASA Astrophysics Data System (ADS)

Lee, Young Jae; Cintora, Pati; Arikkath, Jyothi; Akinsola, Olaoluwa; Kandel, Mikhail; Popescu, Gabriel; Best-Popescu, Catherine

2017-06-01

Optimal growth as well as branching of axons and dendrites is critical for the nervous system function. Neuritic length, arborization, and growth rate determine the innervation properties of neurons and define each cell's computational capability. Thus, to investigate the nervous system function, we need to develop methods and instrumentation techniques capable of quantifying various aspects of neural network formation: neuron process extension, retraction, stability, and branching. During the last three decades, fluorescence microscopy has yielded enormous advances in our understanding of neurobiology. While fluorescent markers provide valuable specificity to imaging, photobleaching, and photoxicity often limit the duration of the investigation. Here, we used spatial light interference microscopy (SLIM) to measure quantitatively neurite outgrowth as a function of cell confluence. Because it is label-free and nondestructive, SLIM allows for long-term investigation over many hours. We found that neurons exhibit a higher growth rate of neurite length in low-confluence versus medium- and high-confluence conditions. We believe this methodology will aid investigators in performing unbiased, nondestructive analysis of morphometric neuronal parameters.

Continuous Flow Liquid Microjunction Surface Sampling Probe Connected On-line with HPLC/MS for Spatially Resolved Analysis of Small Molecules and Proteins

DOE Office of Scientific and Technical Information (OSTI.GOV)

Van Berkel, Gary J; Kertesz, Vilmos

RATIONALE: A continuous flow liquid microjunction surface sampling probe extracts soluble material from surfaces for direct ionization and detection by MS. Demonstrated here is the on-line coupling of such a probe with HPLC/MS enabling extraction, separation and detection of small molecules and proteins from surfaces in a spatially resolved (~0.5 mm diameter spots) manner. Methods: A continuous flow liquid microjunction surface sampling probe was connected to a 6-port, 2-position valve for extract collection and injection to an HPLC column. A QTRAP 5500 hybrid triple quadrupole linear ion trap equipped with a Turbo V ion source operated in positive ESI modemore » was used for all experiments. System operation was tested with extraction, separation and detection of propranolol and associated metabolites from drug dosed tissues and proteins from dried sheep blood spots on paper. Results: Confirmed in the tissue were the parent drug and two different hydroxypropranolol glucuronides. The mass spectrometric response for these compounds from different locations in the liver showed an increase with increasing extraction time (5, 20 and 40 s extractions). For on-line separation and detection/identification of extracted proteins from dried sheep blood spots, two major protein peaks dominated the chromatogram and could be correlated with the expected masses for the hemoglobin and chains. Conclusions: Spatially resolved sampling, separation, and detection of small molecules and proteins from surfaces can be accomplished using a continuous flow liquid microjunction surface sampling probe coupled on-line with HPLC/MS detection.« less

Nanoscale nuclear architecture for cancer diagnosis beyond pathology via spatial-domain low-coherence quantitative phase microscopy

NASA Astrophysics Data System (ADS)

Wang, Pin; Bista, Rajan K.; Khalbuss, Walid E.; Qiu, Wei; Uttam, Shikhar; Staton, Kevin; Zhang, Lin; Brentnall, Teresa A.; Brand, Randall E.; Liu, Yang

2010-11-01

Definitive diagnosis of malignancy is often challenging due to limited availability of human cell or tissue samples and morphological similarity with certain benign conditions. Our recently developed novel technology-spatial-domain low-coherence quantitative phase microscopy (SL-QPM)-overcomes the technical difficulties and enables us to obtain quantitative information about cell nuclear architectural characteristics with nanoscale sensitivity. We explore its ability to improve the identification of malignancy, especially in cytopathologically non-cancerous-appearing cells. We perform proof-of-concept experiments with an animal model of colorectal carcinogenesis-APCMin mouse model and human cytology specimens of colorectal cancer. We show the ability of in situ nanoscale nuclear architectural characteristics in identifying cancerous cells, especially in those labeled as ``indeterminate or normal'' by expert cytopathologists. Our approach is based on the quantitative analysis of the cell nucleus on the original cytology slides without additional processing, which can be readily applied in a conventional clinical setting. Our simple and practical optical microscopy technique may lead to the development of novel methods for early detection of cancer.

Spatially Resolved MaNGA Observations of the Host Galaxy of Superluminous Supernova 2017egm

NASA Astrophysics Data System (ADS)

Chen, Ting-Wan; Schady, Patricia; Xiao, Lin; Eldridge, J. J.; Schweyer, Tassilo; Lee, Chien-Hsiu; Yu, Po-Chieh; Smartt, Stephen J.; Inserra, Cosimo

2017-11-01

Superluminous supernovae (SLSNe) are found predominantly in dwarf galaxies, indicating that their progenitors have a low metallicity. However, the most nearby SLSN to date, SN 2017egm, occurred in the spiral galaxy NGC 3191, which has a relatively high stellar mass and correspondingly high metallicity. In this Letter, we present detailed analysis of the nearby environment of SN 2017egm using MaNGA IFU data, which provides spectral data on kiloparsec scales. From the velocity map we find no evidence that SN 2017egm occurred within some intervening satellite galaxy, and at the SN position most metallicity diagnostics yield a solar and above solar metallicity (12+{log}({{O}}/{{H}})∼ 8.8{--}9.1). Additionally, we measure a small Hα equivalent width (EW) at the SN position of just 34 Å, which is one of the lowest EWs measured at any SLSN or gamma-ray burst position, and indicative of the progenitor star being comparatively old. We also compare the observed properties of NGC 3191 with other SLSN host galaxies. The solar-metallicity environment at the position of SN 2017egm presents a challenge to our theoretical understanding, and our spatially resolved spectral analysis provides further constraints on the progenitors of SLSNe.

Spatially resolving the outer atmosphere of the M giant BK Virginis in the CO first overtone lines with VLTI/AMBER

NASA Astrophysics Data System (ADS)

Ohnaka, K.; Hofmann, K.-H.; Schertl, D.; Weigelt, G.; Malbet, F.; Massi, F.; Meilland, A.; Stee, Ph.

2012-01-01

Context. The mass-loss mechanism in normal K-M giant stars with small variability amplitudes is not yet understood, although the majority among red giant stars are precisely of this type. Aims: We present high-spatial and high-spectral resolution observations of the M7 giant BK Vir with AMBER at the Very Large Telescope Interferometer (VLTI). Our aim is to probe the physical properties of the outer atmosphere by spatially resolving the star in the individual CO first overtone lines. Methods: BK Vir was observed between 2.26 and 2.31 μm using the 16-32-48 m telescope configuration with an angular resolution of 9.8 mas and a spectral resolution of 12 000. Results: The uniform-disk diameters observed in the CO first overtone lines are 12 - 31% larger than those measured in the continuum. We also detected asymmetry in the CO line-forming region, which manifests itself as non-zero/non-π differential and closure phases. The data taken 1.5 months apart show possible time variation on a spatial scale of 30 mas (corresponding to 3 × stellar diameter) at the CO band head. Comparison of the observed data with the MARCS photospheric model shows that whereas the observed CO line spectrum can be satisfactorily reproduced by the model, the angular sizes observed in the CO lines are much larger than predicted by the model. Our model with two additional CO layers above the MARCS photosphere reproduces the observed spectrum and interferometric data in the CO lines simultaneously. This model suggests that the inner CO layer at ~1.2 R⋆ is very dense and warm with a CO column density of ~1022 cm-2 and temperatures of 1900 - 2100 K, while the outer CO layer at 2.5-3.0 R⋆ is characterized by column densities of 1019-1020 cm-2 and temperatures of 1500 - 2100 K. Conclusions: Our AMBER observations of BK Vir have spatially resolved the extended molecular outer atmosphere of a normal M giant in the individual CO lines for the first time. The temperatures derived for the CO layers are

Spatially resolved penetration depth measurements and vortex manipulation in the ferromagnetic superconductor ErNi 2 B 2 C

DOE PAGES

Wulferding, Dirk; Yang, Ilkyu; Yang, Jinho; ...

2015-07-31

We present a local probe study of the magnetic superconductor ErNi 2B 2C, using magnetic force microscopy at sub-Kelvin temperatures. ErNi 2B 2C is an ideal system to explore the effects of concomitant superconductivity and ferromagnetism. At 500 mK, far below the transition to a weakly ferromagnetic state, we directly observe a structured magnetic background on the micrometer scale. We determine spatially resolved absolute values of the magnetic penetration depth λ and study its temperature dependence as the system undergoes magnetic phase transitions from paramagnetic to antiferromagnetic, and to weak ferromagnetic, all within the superconducting regime. We estimate the absolutemore » pinning force of Abrikosov vortices, which shows a position dependence and temperature dependence as well, and discuss the possibility of the purported spontaneous vortex formation.« less

Spatially-resolved carbon flow through a hypersaline phototrophic microbial mat

NASA Astrophysics Data System (ADS)

Moran, J.; Lindemann, S. R.; Cory, A. B.; Courtney, S.; Cole, J. K.; Fredrickson, J.

2013-12-01

analysis (via elemental analysis IRMS and gas bench IRMS) we are employing laser ablation IRMS (LA-IRMS) to provide a spatially-resolved accounting of label uptake through the mat cross section. This technique permits isotope analysis at the 50 μm scale, and can provide multiple isotope analyses within each mat strata. By coupling LA-IRMS analysis with laminar sectioning of the mat and amplicon sequencing of the rrnA gene, we seek to establish linkages between phylogeny and function over the course of a diel cycle with highlighted emphasis on evidence of carbon transfer between mat laminae and the phylotypes that inhabit them. We are also using a series of carbon accumulation microcosms to quantify net carbon fixation over the seasonal cycle. These microcosms are deployed at multiple depths to provide an accounting of carbon cycling under the specific geochemical conditions experienced at variable depth. Coupling the data from these individual microcosms to our bathymetric survey of Hot Lake permits us to estimate total mat carbon fixation, and therefore to begin to assess the impact of the mat on the greater lake carbon cycle.

Spatially Resolved Metal Gas Clouds

NASA Astrophysics Data System (ADS)

Péroux, C.; Rahmani, H.; Arrigoni Battaia, F.; Augustin, R.

2018-05-01

We now have mounting evidences that the circumgalactic medium (CGM) of galaxies is polluted with metals processed through stars. The fate of these metals is however still an open question and several findings indicate that they remain poorly mixed. A powerful tool to study the low-density gas of the CGM is offered by absorption lines in quasar spectra, although the information retrieved is limited to 1D along the sightline. We report the serendipitous discovery of two close-by bright zgal=1.148 extended galaxies with a fortuitous intervening zabs=1.067 foreground absorber. MUSE IFU observations spatially probes kpc-scales in absorption in the plane of the sky over a total area spanning ˜30 kpc-2. We identify two [O II] emitters at zabs down to 21 kpc with SFR˜2 M⊙/yr. We measure small fractional variations (<30%) in the equivalent widths of Fe II and Mg II cold gas absorbers on coherence scales of 8kpc but stronger variation on larger scales (25kpc). We compute the corresponding cloud gas mass <2 × 109M⊙. Our results indicate a good efficiency of the metal mixing on kpc-scales in the CGM of a typical z˜1 galaxy. This study show-cases new prospects for mapping the distribution and sizes of metal clouds observed in absorption against extended background sources with 3D spectroscopy.

A radially accessible tubular in situ X-ray cell for spatially resolved operando scattering and spectroscopic studies of electrochemical energy storage devices

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Hao; Allan, Phoebe K.; Borkiewicz, Olaf J.

2016-09-16

A tubularoperandoelectrochemical cell has been developed to allow spatially resolved X-ray scattering and spectroscopic measurements of individual cell components, or regions thereof, during device operation. These measurements are enabled by the tubular cell geometry, wherein the X-ray-transparent tube walls allow radial access for the incident and scattered/transmitted X-ray beam; by probing different depths within the electrode stack, the transformation of different components or regions can be resolved. The cell is compatible with a variety of synchrotron-based scattering, absorption and imaging methodologies. The reliability of the electrochemical cell and the quality of the resulting X-ray scattering and spectroscopic data are demonstratedmore » for two types of energy storage: the evolution of the distribution of the state of charge of an Li-ion battery electrode during cycling is documented using X-ray powder diffraction, and the redistribution of ions between two porous carbon electrodes in an electrochemical double-layer capacitor is documented using X-ray absorption near-edge spectroscopy.« less

A Spatially Resolved Investigation on the Influence of AGN and Star Formation in a Lensed Main-Sequence Galaxy at z = 2.39

NASA Astrophysics Data System (ADS)

Fischer, Travis; Rigby, Jane; Gladders, Michael; Sharon, Keren q.; Barrientos, L. Felipe; Bayliss, Matt; Dahle, Håkon; Florian, Michael; Johnson, Traci Lin; Wuyts, Eva

2018-01-01

We present rest-frame optical SINFONI integral field spectroscopy and rest-frame UV HST imaging of a lensed galaxy hosting an active galactic nucleus (AGN) at z = 2.39. Galactic wind feedback is widely acknowledged to play a critical role in the evolution of galaxies, however, the physical mechanisms involved and the relative importance of AGN and star formation as the main feedback drivers remain poorly understood. AGN-driven feedback has been evident in very luminous but rare quasars and radio galaxies, but observational evidence remains lacking for less extreme, “normal” star-forming galaxies. We report, for the first time at high redshift, spatially resolved velocity profiles and geometries of an AGN-driven outflow in a normal star-forming galaxy and spatial extents and morphologies of Lyα emission and stellar UV continuum. Analyzing these measurements in tandem, we determine the physical conditions, geometry, and excitation sources of the interstellar medium in a star-forming, AGN-hosting galaxy at cosmic noon.

Resolving the Quantitative-Qualitative Dilemma: A Critical Realist Approach

ERIC Educational Resources Information Center

Scott, David

2007-01-01

The philosophical issues underpinning the quantitative-qualitative divide in educational research are examined. Three types of argument which support a resolution are considered: pragmatism, false duality and warranty through triangulation. In addition a number of proposed strategies--alignment, sequencing, translation and triangulation--are…

Spatially Resolved Carbon Isotope and Elemental Analyses of the Root-Rhizosphere-Soil System to Understand Below-ground Nutrient Interactions

NASA Astrophysics Data System (ADS)

Denis, E. H.; Ilhardt, P.; Tucker, A. E.; Huggett, N. L.; Rosnow, J. J.; Krogstad, E. J.; Moran, J.

2017-12-01

, soil, and specific types of mineral grains within soil. Integrating spatially resolved analysis of photosynthate distribution with local geochemical microenvironments may reveal key properties of nutrient exchange hotspots that help direct overall plant health and productivity.

Energy dissipation mechanism revealed by spatially resolved Raman thermometry of graphene/hexagonal boron nitride heterostructure devices

NASA Astrophysics Data System (ADS)

Kim, Daehee; Kim, Hanul; Yun, Wan Soo; Watanabe, Kenji; Taniguchi, Takashi; Rho, Heesuk; Bae, Myung-Ho

2018-04-01

Understanding the energy transport by charge carriers and phonons in two-dimensional (2D) van der Waals heterostructures is essential for the development of future energy-efficient 2D nanoelectronics. Here, we performed in situ spatially resolved Raman thermometry on an electrically biased graphene channel and its hBN substrate to study the energy dissipation mechanism in graphene/hBN heterostructures. By comparing the temperature profile along the biased graphene channel with that along the hBN substrate, we found that the thermal boundary resistance between the graphene and hBN was in the range of (1-2) ~ × 10-7 m2 K W-1 from ~100 °C to the onset of graphene break-down at ~600 °C in air. Consideration of an electro-thermal transport model together with the Raman thermometry conducted in air showed that a doping effect occurred under a strong electric field played a crucial role in the energy dissipation of the graphene/hBN device up to T ~ 600 °C.

The MUSE Hubble Ultra Deep Field Survey. V. Spatially resolved stellar kinematics of galaxies at redshift 0.2 ≲ z ≲ 0.8

NASA Astrophysics Data System (ADS)

Guérou, Adrien; Krajnović, Davor; Epinat, Benoit; Contini, Thierry; Emsellem, Eric; Bouché, Nicolas; Bacon, Roland; Michel-Dansac, Leo; Richard, Johan; Weilbacher, Peter M.; Schaye, Joop; Marino, Raffaella Anna; den Brok, Mark; Erroz-Ferrer, Santiago

2017-11-01

We present spatially resolved stellar kinematic maps, for the first time, for a sample of 17 intermediate redshift galaxies (0.2 ≲ z ≲ 0.8). We used deep MUSE/VLT integral field spectroscopic observations in the Hubble Deep Field South (HDFS) and Hubble Ultra Deep Field (HUDF), resulting from ≈30 h integration time per field, each covering 1' × 1' field of view, with ≈ 0.̋65 spatial resolution. We selected all galaxies brighter than 25 mag in the I band and for which the stellar continuum is detected over an area that is at least two times larger than the spatial resolution. The resulting sample contains mostly late-type disk, main-sequence star-forming galaxies with 108.5 M⊙ ≲ M∗ ≲ 1010.5 M⊙. Using a full-spectrum fitting technique, we derive two-dimensional maps of the stellar and gas kinematics, including the radial velocity V and velocity dispersion σ. We find that most galaxies in the sample are consistent with having rotating stellar disks with roughly constant velocity dispersions and that the second order velocity moments Vrms = √V2+σ2 of the gas and stars, a scaling proxy for the galaxy gravitational potential, compare well to each other. These spatially resolved observations of the stellar kinematics of intermediate redshift galaxies suggest that the regular stellar kinematics of disk galaxies that is observed in the local Universe was already in place 4-7 Gyr ago and that their gas kinematics traces the gravitational potential of the galaxy, thus is not dominated by shocks and turbulent motions. Finally, we build dynamical axisymmetric Jeans models constrained by the derived stellar kinematics for two specific galaxies and derive their dynamical masses. These are in good agreement (within 25%) with those derived from simple exponential disk models based on the gas kinematics. The obtained mass-to-light ratios hint towards dark matter dominated systems within a few effective radii. Based on observations made with ESO telescopes at

The History of the M31 Disk from Resolved Stellar Populations as Seen by PHAT

NASA Astrophysics Data System (ADS)

Lewis, A. R.; Dalcanton, J. J.; Dolphin, A. E.; Weisz, D. R.; Williams, B. F.; PHAT Collaboration

2014-03-01

The Panchromatic Hubble Andromeda Treasury (PHAT) is an HST multi-cycle treasury program that is mapping the resolved stellar populations of ˜1/3 of M31 from the UV through the near-IR. These data provide color and luminosity information for more than 150 million stars in the M31 disk. We use stellar evolution models to fit the luminous main sequence to derive spatially-resolved recent star formation histories (SFHs) over large areas of M31 with 50-100 pc resolution. These include individual star-forming regions as well as quiescent portions of the disk. We use the gridded SFHs to create movies of star formation activity to study the evolution of individual star-forming events across the disk. Outside of the star-forming regions, we use our resolved stellar photometry to derive the full SFHs of larger regions. These allow us to probe spatial and temporal trends in age and metallicity across a large radial baseline, providing constraints on the global formation and evolution of the disk over a Hubble time. M31 is the only large disk galaxy that is close enough to obtain the photometry necessary for this type of spatially-resolved SFH mapping.

Quantitation of spatially-localized proteins in tissue samples using MALDI-MRM imaging.

PubMed

Clemis, Elizabeth J; Smith, Derek S; Camenzind, Alexander G; Danell, Ryan M; Parker, Carol E; Borchers, Christoph H

2012-04-17

MALDI imaging allows the creation of a "molecular image" of a tissue slice. This image is reconstructed from the ion abundances in spectra obtained while rastering the laser over the tissue. These images can then be correlated with tissue histology to detect potential biomarkers of, for example, aberrant cell types. MALDI, however, is known to have problems with ion suppression, making it difficult to correlate measured ion abundance with concentration. It would be advantageous to have a method which could provide more accurate protein concentration measurements, particularly for screening applications or for precise comparisons between samples. In this paper, we report the development of a novel MALDI imaging method for the localization and accurate quantitation of proteins in tissues. This method involves optimization of in situ tryptic digestion, followed by reproducible and uniform deposition of an isotopically labeled standard peptide from a target protein onto the tissue, using an aerosol-generating device. Data is acquired by MALDI multiple reaction monitoring (MRM) mass spectrometry (MS), and accurate peptide quantitation is determined from the ratio of MRM transitions for the endogenous unlabeled proteolytic peptides to the corresponding transitions from the applied isotopically labeled standard peptides. In a parallel experiment, the quantity of the labeled peptide applied to the tissue was determined using a standard curve generated from MALDI time-of-flight (TOF) MS data. This external calibration curve was then used to determine the quantity of endogenous peptide in a given area. All standard curves generate by this method had coefficients of determination greater than 0.97. These proof-of-concept experiments using MALDI MRM-based imaging show the feasibility for the precise and accurate quantitation of tissue protein concentrations over 2 orders of magnitude, while maintaining the spatial localization information for the proteins.

Quantitative study of energy-transfer mechanism in Eu,O-codoped GaN by time-resolved photoluminescence spectroscopy

NASA Astrophysics Data System (ADS)

Inaba, Tomohiro; Kojima, Takanori; Yamashita, Genki; Matsubara, Eiichi; Mitchell, Brandon; Miyagawa, Reina; Eryu, Osamu; Tatebayashi, Jun; Ashida, Masaaki; Fujiwara, Yasufumi

2018-04-01

In order to investigate the excitation processes in Eu,O-codoped GaN (GaN:Eu,O), the time-resolved photoluminescence signal including the rising part is analyzed. A rate equation is developed based upon a model for the excitation processes in GaN:Eu to fit the experimental data. The non-radiative recombination rate of the trap state in the GaN host, the energy transfer rate between the Eu3+ ions and the GaN host, the radiative transition probability of Eu3+ ion, as well as the ratio of the number of luminescent sites (OMVPE 4α and OMVPE 4β), are simultaneously determined. It is revealed and quantified that radiative transition probability of the Eu ion is the bottleneck for the enhancement of light output from GaN:Eu. We also evaluate the effect of the growth conditions on the luminescent efficiency of GaN:Eu quantitatively, and find the correlation between emission intensity of GaN:Eu and the fitting parameters introduced in our model.

Sub-nanosecond time-resolved near-field scanning magneto-optical microscope.

PubMed

Rudge, J; Xu, H; Kolthammer, J; Hong, Y K; Choi, B C

2015-02-01

We report on the development of a new magnetic microscope, time-resolved near-field scanning magneto-optical microscope, which combines a near-field scanning optical microscope and magneto-optical contrast. By taking advantage of the high temporal resolution of time-resolved Kerr microscope and the sub-wavelength spatial resolution of a near-field microscope, we achieved a temporal resolution of ∼50 ps and a spatial resolution of <100 nm. In order to demonstrate the spatiotemporal magnetic imaging capability of this microscope, the magnetic field pulse induced gyrotropic vortex dynamics occurring in 1 μm diameter, 20 nm thick CoFeB circular disks has been investigated. The microscope provides sub-wavelength resolution magnetic images of the gyrotropic motion of the vortex core at a resonance frequency of ∼240 MHz.

Spectrally And Temporally Resolved Low-Light Level Video Microscopy

NASA Astrophysics Data System (ADS)

Wampler, John E.; Furukawa, Ruth; Fechheimer, Marcus

1989-12-01

The IDG law-light video microscope system was designed to aid studies of localization of subcellular luminescence sources and stimulus/response coupling in single living cells using luminescent probes. Much of the motivation for design of this instrument system came from the pioneering efforts of Dr. Reynolds (Reynolds, Q. Rev. Biophys. 5, 295-347; Reynolds and Taylor, Bioscience 30, 586-592) who showed the value of intensified video camera systems for detection and localizion of fluorescence and bioluminescence signals from biological tissues. Our instrument system has essentially two roles, 1) localization and quantitation of very weak bioluminescence signals and 2) quantitation of intracellular environmental characteristics such as pH and calcium ion concentrations using fluorescent and bioluminescent probes. The instrument system exhibits over one million fold operating range allowing visualization and enhancement of quantum limited images with quantum limited response, spectral analysis of fluorescence signals, and transmitted light imaging. The computer control of the system implements rapid switching between light regimes, spatially resolved spectral scanning, and digital data processing for spectral shape analysis and for detailed analysis of the statistical distribution of single cell measurements. The system design and software algorithms used by the system are summarized. These design criteria are illustrated with examples taken from studies of bioluminescence, applications of bioluminescence to study developmental processes and gene expression in single living cells, and applications of fluorescent probes to study stimulus/response coupling in living cells.

Time-resolved Sensing of Meso-scale Shock Compression with Multilayer Photonic Crystal Structures

NASA Astrophysics Data System (ADS)

Scripka, David; Lee, Gyuhyon; Summers, Christopher J.; Thadhani, Naresh

2017-06-01

Multilayer Photonic Crystal structures can provide spatially and temporally resolved data needed to validate theoretical and computational models relevant for understanding shock compression in heterogeneous materials. Two classes of 1-D photonic crystal multilayer structures were studied: optical microcavities (OMC) and distributed Bragg reflectors (DBR). These 0.5 to 5 micron thick structures were composed of SiO2, Al2O3, Ag, and PMMA layers fabricated primarily via e-beam evaporation. The multilayers have unique spectral signatures inherently linked to their time-resolved physical states. By observing shock-induced changes in these signatures, an optically-based pressure sensor was developed. Results to date indicate that both OMCs and DBRs exhibit nanosecond-resolved spectral shifts of several to 10s of nanometers under laser-driven shock compression loads of 0-10 GPa, with the magnitude of the shift strongly correlating to the shock load magnitude. Additionally, spatially and temporally resolved spectral shifts under heterogeneous laser-driven shock compression created by partial beam blocking have been successfully demonstrated. These results illustrate the potential for multilayer structures to serve as meso-scale sensors, capturing temporal and spatial pressure profile evolutions in shock-compressed heterogeneous materials, and revealing meso-scale pressure distributions across a shocked surface. Supported by DTRA Grant HDTRA1-12-1-005 and DoD, AFOSR, National Defense Science and Eng. Graduate Fellowship, 32 CFR 168a.

Compact all-fiber quartz-enhanced photoacoustic spectroscopy sensor with a 30.72 kHz quartz tuning fork and spatially resolved trace gas detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ma, Yufei, E-mail: mayufei@hit.edu.cn; Post-doctoral Mobile Station of Power Engineering and Engineering Thermophysics, Harbin Institute of Technology, Harbin 150001; He, Ying

An ultra compact all-fiber quartz-enhanced photoacoustic spectroscopy (QEPAS) sensor using quartz tuning fork (QTF) with a low resonance frequency of 30.72 kHz was demonstrated. Such a sensor architecture has the advantages of easier optical alignment, lower insertion loss, lower cost, and more compact compared with a conventional QEPAS sensor using discrete optical components for laser delivery and coupling to the QTF. A fiber beam splitter and three QTFs were employed to perform multi-point detection and demonstrated the potential of spatially resolved measurements.

Wide-field high spatial frequency domain imaging of tissue microstructure

NASA Astrophysics Data System (ADS)

Lin, Weihao; Zeng, Bixin; Cao, Zili; Zhu, Danfeng; Xu, M.

2018-02-01

Wide-field tissue imaging is usually not capable of resolving tissue microstructure. We present High Spatial Frequency Domain Imaging (HSFDI) - a noncontact imaging modality that spatially maps the tissue microscopic scattering structures over a large field of view. Based on an analytical reflectance model of sub-diffusive light from forward-peaked highly scattering media, HSFDI quantifies the spatially-resolved parameters of the light scattering phase function from the reflectance of structured light modulated at high spatial frequencies. We have demonstrated with ex vivo cancerous tissue to validate the robustness of HSFDI in significant contrast and differentiation of the microstructutral parameters between different types and disease states of tissue.

Spatially resolved spectroscopy of non-thermal X-rays in RX J1713.7-3946 with Chandra

NASA Astrophysics Data System (ADS)

Okuno, Tomoyuki; Tanaka, Takaaki; Uchida, Hiroyuki; Matsumura, Hideaki; Tsuru, Takeshi Go

2018-06-01

The young shell-type supernova remnant (SNR) RX J1713.7-3946 has been studied as a suitable target to test the SNR paradigm for the origin of Galactic cosmic rays. We present a spatially resolved spectroscopy of the non-thermal X-ray emission in RX J1713.7-3946 with Chandra. In order to obtain X-ray properties of the filamentary structures and their surrounding regions, we divide the southeastern (SE), southwestern (SW), and northwestern (NW) parts of the SNR into subregions on the typical order of several 10" and extract spectra from each subregion. Their photon indices are significantly different among the subregions, with a range of 1.8 < Γ < 3. In the SE part, the clear filaments are harder (Γ ˜ 2.0) than the surrounding regions. This is a common feature often observed in young SNRs and naturally interpreted as a consequence of synchrotron cooling. On the other hand, the bright filamentary regions do not necessarily coincide with the hardest regions in the SW and NW parts. We also find the SW filamentary region is relatively rather soft (Γ ˜ 2.7). In addition, we find that hard regions with photon indices of 2.0-2.2 exist around the bright emission although the hard regions lie in the downstream region and the bright emission does not appear to be the blast wave shock front. Both the aforementioned characteristic regions in SW are located close to peaks of the interstellar gas. We discuss possible origins of the spatial variation of the photon indices, paying particular attention to the shock-cloud interactions.

Visualizing spatial distribution of alectinib in murine brain using quantitative mass spectrometry imaging.

PubMed

Aikawa, Hiroaki; Hayashi, Mitsuhiro; Ryu, Shoraku; Yamashita, Makiko; Ohtsuka, Naoto; Nishidate, Masanobu; Fujiwara, Yasuhiro; Hamada, Akinobu

2016-03-30

In the development of anticancer drugs, drug concentration measurements in the target tissue have been thought to be crucial for predicting drug efficacy and safety. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is commonly used for determination of average drug concentrations; however, complete loss of spatial information in the target tissue occurs. Mass spectrometry imaging (MSI) has been recently applied as an innovative tool for detection of molecular distribution of pharmacological agents in heterogeneous targets. This study examined the intra-brain transitivity of alectinib, a novel anaplastic lymphoma kinase inhibitor, using a combination of matrix-assisted laser desorption ionization-MSI and LC-MS/MS techniques. We first analyzed the pharmacokinetic profiles in FVB mice and then examined the effect of the multidrug resistance protein-1 (MDR1) using Mdr1a/b knockout mice including quantitative distribution of alectinib in the brain. While no differences were observed between the mice for the plasma alectinib concentrations, diffuse alectinib distributions were found in the brain of the Mdr1a/b knockout versus FVB mice. These results indicate the potential for using quantitative MSI for clarifying drug distribution in the brain on a microscopic level, in addition to suggesting a possible use in designing studies for anticancer drug development and translational research.

Visualizing spatial distribution of alectinib in murine brain using quantitative mass spectrometry imaging

PubMed Central

Aikawa, Hiroaki; Hayashi, Mitsuhiro; Ryu, Shoraku; Yamashita, Makiko; Ohtsuka, Naoto; Nishidate, Masanobu; Fujiwara, Yasuhiro; Hamada, Akinobu

2016-01-01

In the development of anticancer drugs, drug concentration measurements in the target tissue have been thought to be crucial for predicting drug efficacy and safety. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is commonly used for determination of average drug concentrations; however, complete loss of spatial information in the target tissue occurs. Mass spectrometry imaging (MSI) has been recently applied as an innovative tool for detection of molecular distribution of pharmacological agents in heterogeneous targets. This study examined the intra-brain transitivity of alectinib, a novel anaplastic lymphoma kinase inhibitor, using a combination of matrix-assisted laser desorption ionization–MSI and LC-MS/MS techniques. We first analyzed the pharmacokinetic profiles in FVB mice and then examined the effect of the multidrug resistance protein-1 (MDR1) using Mdr1a/b knockout mice including quantitative distribution of alectinib in the brain. While no differences were observed between the mice for the plasma alectinib concentrations, diffuse alectinib distributions were found in the brain of the Mdr1a/b knockout versus FVB mice. These results indicate the potential for using quantitative MSI for clarifying drug distribution in the brain on a microscopic level, in addition to suggesting a possible use in designing studies for anticancer drug development and translational research. PMID:27026287

The quantitative modelling of human spatial habitability

NASA Technical Reports Server (NTRS)

Wise, James A.

1988-01-01

A theoretical model for evaluating human spatial habitability (HuSH) in the proposed U.S. Space Station is developed. Optimizing the fitness of the space station environment for human occupancy will help reduce environmental stress due to long-term isolation and confinement in its small habitable volume. The development of tools that operationalize the behavioral bases of spatial volume for visual kinesthetic, and social logic considerations is suggested. This report further calls for systematic scientific investigations of how much real and how much perceived volume people need in order to function normally and with minimal stress in space-based settings. The theoretical model presented in this report can be applied to any size or shape interior, at any scale of consideration, for the Space Station as a whole to an individual enclosure or work station. Using as a point of departure the Isovist model developed by Dr. Michael Benedikt of the U. of Texas, the report suggests that spatial habitability can become as amenable to careful assessment as engineering and life support concerns.

Spectral and spatial resolving of photoelectric property of femtosecond laser drilled holes of GaSb(1-x)Bi(x).

PubMed

Pan, C B; Zha, F X; Song, Y X; Shao, J; Dai, Y; Chen, X R; Ye, J Y; Wang, S M

2015-07-15

Femtosecond laser drilled holes of GaSbBi were characterized by the joint measurements of photoconductivity (PC) spectroscopy and laser-beam-induced current (LBIC) mapping. The excitation light in PC was focused down to 60 μm presenting the spectral information of local electronic property of individual holes. A redshift of energy band edge of about 6-8 meV was observed by the PC measurement when the excitation light irradiated on the laser drilled holes. The spatial resolving of photoelectric property was achieved by the LBIC mapping which shows "pseudo-holes" with much larger dimensions than the geometric sizes of the holes. The reduced LBIC current with the pseudo-holes is associated with the redshift effect indicating that the electronic property of the rim areas of the holes is modified by the femtosecond laser drilling.

Quantitative protein localization signatures reveal an association between spatial and functional divergences of proteins.

PubMed

Loo, Lit-Hsin; Laksameethanasan, Danai; Tung, Yi-Ling

2014-03-01

Protein subcellular localization is a major determinant of protein function. However, this important protein feature is often described in terms of discrete and qualitative categories of subcellular compartments, and therefore it has limited applications in quantitative protein function analyses. Here, we present Protein Localization Analysis and Search Tools (PLAST), an automated analysis framework for constructing and comparing quantitative signatures of protein subcellular localization patterns based on microscopy images. PLAST produces human-interpretable protein localization maps that quantitatively describe the similarities in the localization patterns of proteins and major subcellular compartments, without requiring manual assignment or supervised learning of these compartments. Using the budding yeast Saccharomyces cerevisiae as a model system, we show that PLAST is more accurate than existing, qualitative protein localization annotations in identifying known co-localized proteins. Furthermore, we demonstrate that PLAST can reveal protein localization-function relationships that are not obvious from these annotations. First, we identified proteins that have similar localization patterns and participate in closely-related biological processes, but do not necessarily form stable complexes with each other or localize at the same organelles. Second, we found an association between spatial and functional divergences of proteins during evolution. Surprisingly, as proteins with common ancestors evolve, they tend to develop more diverged subcellular localization patterns, but still occupy similar numbers of compartments. This suggests that divergence of protein localization might be more frequently due to the development of more specific localization patterns over ancestral compartments than the occupation of new compartments. PLAST enables systematic and quantitative analyses of protein localization-function relationships, and will be useful to elucidate protein

Quantitative Protein Localization Signatures Reveal an Association between Spatial and Functional Divergences of Proteins

PubMed Central

Loo, Lit-Hsin; Laksameethanasan, Danai; Tung, Yi-Ling

2014-01-01

Protein subcellular localization is a major determinant of protein function. However, this important protein feature is often described in terms of discrete and qualitative categories of subcellular compartments, and therefore it has limited applications in quantitative protein function analyses. Here, we present Protein Localization Analysis and Search Tools (PLAST), an automated analysis framework for constructing and comparing quantitative signatures of protein subcellular localization patterns based on microscopy images. PLAST produces human-interpretable protein localization maps that quantitatively describe the similarities in the localization patterns of proteins and major subcellular compartments, without requiring manual assignment or supervised learning of these compartments. Using the budding yeast Saccharomyces cerevisiae as a model system, we show that PLAST is more accurate than existing, qualitative protein localization annotations in identifying known co-localized proteins. Furthermore, we demonstrate that PLAST can reveal protein localization-function relationships that are not obvious from these annotations. First, we identified proteins that have similar localization patterns and participate in closely-related biological processes, but do not necessarily form stable complexes with each other or localize at the same organelles. Second, we found an association between spatial and functional divergences of proteins during evolution. Surprisingly, as proteins with common ancestors evolve, they tend to develop more diverged subcellular localization patterns, but still occupy similar numbers of compartments. This suggests that divergence of protein localization might be more frequently due to the development of more specific localization patterns over ancestral compartments than the occupation of new compartments. PLAST enables systematic and quantitative analyses of protein localization-function relationships, and will be useful to elucidate protein

SDSS-IV MaNGA: The Spatially Resolved Stellar Initial Mass Function in ˜400 Early-Type Galaxies

NASA Astrophysics Data System (ADS)

Parikh, Taniya; Thomas, Daniel; Maraston, Claudia; Westfall, Kyle B.; Goddard, Daniel; Lian, Jianhui; Meneses-Goytia, Sofia; Jones, Amy; Vaughan, Sam; Andrews, Brett H.; Bershady, Matthew; Bizyaev, Dmitry; Brinkmann, Jonathan; Brownstein, Joel R.; Bundy, Kevin; Drory, Niv; Emsellem, Eric; Law, David R.; Newman, Jeffrey A.; Roman-Lopes, Alexandre; Wake, David; Yan, Renbin; Zheng, Zheng

2018-03-01

MaNGA provides the opportunity to make precise spatially resolved measurements of the IMF slope in galaxies owing to its unique combination of spatial resolution, wavelength coverage and sample size. We derive radial gradients in age, element abundances and IMF slope analysing optical and near-infrared absorption features from stacked spectra out to the half-light radius of 366 early-type galaxies with masses 9.9 - 10.8 log M/M⊙. We find flat gradients in age and [α/Fe] ratio, as well as negative gradients in metallicity, consistent with the literature. We further derive significant negative gradients in the [Na/Fe] ratio with galaxy centres being well enhanced in Na abundance by up to 0.5 dex. Finally, we find a gradient in IMF slope with a bottom-heavy IMF in the centre (typical mass excess factor of 1.5) and a Milky Way-type IMF at the half-light radius. This pattern is mass-dependent with the lowest mass galaxies in our sample featuring only a shallow gradient around a Milky Way IMF. Our results imply the local IMF-σ relation within galaxies to be even steeper than the global relation and hint towards the local metallicity being the dominating factor behind the IMF variations. We also employ different stellar population models in our analysis and show that a radial IMF gradient is found independently of the stellar population model used. A similar analysis of the Wing-Ford band provides inconsistent results and further evidence of the difficulty in measuring and modelling this particular feature.

Solid-state selective (13)C excitation and spin diffusion NMR to resolve spatial dimensions in plant cell walls.

PubMed

Foston, Marcus; Katahira, Rui; Gjersing, Erica; Davis, Mark F; Ragauskas, Arthur J

2012-02-15

The average spatial dimensions between major biopolymers within the plant cell wall can be resolved using a solid-state NMR technique referred to as a (13)C cross-polarization (CP) SELDOM (selectively by destruction of magnetization) with a mixing time delay for spin diffusion. Selective excitation of specific aromatic lignin carbons indicates that lignin is in close proximity to hemicellulose followed by amorphous and finally crystalline cellulose. (13)C spin diffusion time constants (T(SD)) were extracted using a two-site spin diffusion theory developed for (13)C nuclei under magic angle spinning (MAS) conditions. These time constants were then used to calculate an average lower-limit spin diffusion length between chemical groups within the plant cell wall. The results on untreated (13)C enriched corn stover stem reveal that the lignin carbons are, on average, located at distances ∼0.7-2.0 nm from the carbons in hemicellulose and cellulose, whereas the pretreated material had larger separations.

Diagnostic performance of semi-quantitative and quantitative stress CMR perfusion analysis: a meta-analysis.

PubMed

van Dijk, R; van Assen, M; Vliegenthart, R; de Bock, G H; van der Harst, P; Oudkerk, M

2017-11-27

Stress cardiovascular magnetic resonance (CMR) perfusion imaging is a promising modality for the evaluation of coronary artery disease (CAD) due to high spatial resolution and absence of radiation. Semi-quantitative and quantitative analysis of CMR perfusion are based on signal-intensity curves produced during the first-pass of gadolinium contrast. Multiple semi-quantitative and quantitative parameters have been introduced. Diagnostic performance of these parameters varies extensively among studies and standardized protocols are lacking. This study aims to determine the diagnostic accuracy of semi- quantitative and quantitative CMR perfusion parameters, compared to multiple reference standards. Pubmed, WebOfScience, and Embase were systematically searched using predefined criteria (3272 articles). A check for duplicates was performed (1967 articles). Eligibility and relevance of the articles was determined by two reviewers using pre-defined criteria. The primary data extraction was performed independently by two researchers with the use of a predefined template. Differences in extracted data were resolved by discussion between the two researchers. The quality of the included studies was assessed using the 'Quality Assessment of Diagnostic Accuracy Studies Tool' (QUADAS-2). True positives, false positives, true negatives, and false negatives were subtracted/calculated from the articles. The principal summary measures used to assess diagnostic accuracy were sensitivity, specificity, andarea under the receiver operating curve (AUC). Data was pooled according to analysis territory, reference standard and perfusion parameter. Twenty-two articles were eligible based on the predefined study eligibility criteria. The pooled diagnostic accuracy for segment-, territory- and patient-based analyses showed good diagnostic performance with sensitivity of 0.88, 0.82, and 0.83, specificity of 0.72, 0.83, and 0.76 and AUC of 0.90, 0.84, and 0.87, respectively. In per territory

HST Spatially Resolved Spectra of the Accretion Disc and Gas Stream of the Nova-Like Variable UX Ursae Majoris

NASA Technical Reports Server (NTRS)

Baptista, Raymundo; Horne, Keith; Wade, Richard A.; Hubeny, Ivan; Long, Knox S.; Rutten, Rene G. M.

1998-01-01

Time-resolved eclipse spectroscopy of the nova-like variable UX UMa obtained with the Hubble Space Telescope/Faint Object Spectrograph (HST/FOS) on 1994 August and November is analysed with eclipse mapping techniques to produce spatially resolved spectra of its accretion disk and gas stream as a function of distance from the disk centre. The inner accretion disk is characterized by a blue continuum filled with absorption bands and lines, which cross over to emission with increasing disk radius, similar to that reported at optical wavelengths. The comparison of spatially resolved spectra at different azimuths reveals a significant asymmetry in the disk emission at ultraviolet (UV) wavelengths, with the disk side closest to the secondary star showing pronounced absorption by an 'iron curtain' and a Balmer jump in absorption. These results suggest the existence of an absorbing ring of cold gas whose density and/or vertical scale increase with disk radius. The spectrum of the infalling gas stream is noticeably different from the disc spectrum at the same radius suggesting that gas overflows through the impact point at the disk rim and continues along the stream trajectory, producing distinct emission down to 0.1 R(sub LI). The spectrum of the uneclipsed light shows prominent emission lines of Lyalpha, N v lambda1241, SiIV Lambda 1400, C IV Lambda 1550, HeII Lambda 1640, and MgII Lambda 2800, and a UV continuum rising towards longer wavelengths. The Balmer jump appears clearly in emission indicating that the uneclipsed light has an important contribution from optically thin gas. The lines and optically thin continuum emission are most probably emitted in a vertically extended disk chromosphere + wind. The radial temperature profiles of the continuum maps are well described by a steady-state disc model in the inner and intermediate disk regions (R greater than or equal to 0.3R(sub LI) ). There is evidence of an increase in the mass accretion rate from August to November

Determination of atomic positions from time resolved high resolution transmission electron microscopy images.

PubMed

Hussaini, Zahra; Lin, Pin Ann; Natarajan, Bharath; Zhu, Wenhui; Sharma, Renu

2018-03-01

For many reaction processes, such as catalysis, phase transformations, nanomaterial synthesis etc., nanoscale observations at high spatial (sub-nanometer) and temporal (millisecond) resolution are required to characterize and comprehend the underlying factors that favor one reaction over another. The combination of such spatial and temporal resolution (up to 600 µs), while rich in information, produces a large number of snapshots, each of which must be analyzed to obtain the structural (and thereby chemical) information. Here we present a methodology for automated quantitative measurement of real-time atomic position fluctuations in a nanoparticle. We leverage a combination of several image processing algorithms to precisely identify the positions of the atomic columns in each image. A geometric model is then used to measure the time-evolution of distances and angles between neighboring atomic columns to identify different phases and quantify local structural fluctuations. We apply this technique to determine the atomic-level fluctuations in the relative fractions of metal and metal-carbide phases in a cobalt catalyst nanoparticle during single-walled carbon nanotube (SWCNT) growth. These measurements provided a means to obtain the number of carbon atoms incorporated into and released from the catalyst particle, thereby helping resolve carbon reaction pathways during SWCNT growth. Further we demonstrate the use of this technique to measure the reaction kinetics of iron oxide reduction. Apart from reducing the data analysis time, the statistical approach allows us to measure atomic distances with sub-pixel resolution. We show that this method can be applied universally to measure atomic positions with a precision of 0.01 nm from any set of atomic-resolution video images. With the advent of high time-resolution direct detection cameras, we anticipate such methods will be essential in addressing the metrology problem of quantifying large datasets of time-resolved

A Spatially Resolved Plerionic X-Ray Nebula around PSR B0540-69.

PubMed

Gotthelf; Wang

2000-04-01

We present a high-resolution Chandra X-ray observation of PSR B0540-69, the Crab-like 50 ms pulsar in the Large Magellanic Cloud. We use phase-resolved imaging to decompose the extended X-ray emission, as expected of a synchrotron nebula, from the pointlike emission of the pulsar. The image of the pulsed X-ray emission shows a well-defined point-spread function of the observation, while the resolved nebula has a morphology and size remarkably similar to the Crab nebula, including evidence for a jetlike feature from PSR B0540-69. The patchy outer shell, which most likely represents the expanding blast wave of the supernova, is reminiscent of that seen in radio. Based on morphology, size, and energetics, there can be little doubt that SNR B0540-69 is an analogous system to the Crab but located in our neighboring galaxy.

Quantitative spatial frequency fluorescence imaging in the sub-diffusive domain for image-guided glioma resection

PubMed Central

Sibai, Mira; Veilleux, Israel; Elliott, Jonathan T.; Leblond, Frederic; Wilson, Brian C.

2015-01-01

Intraoperative 5- aminolevulinic acid induced-Protoporphyrin IX (PpIX) fluorescence guidance enables maximum safe resection of glioblastomas by providing surgeons with real-time tumor optical contrast. However, visual assessment of PpIX fluorescence is subjective and limited by the distorting effects of light attenuation and tissue autofluorescence. We have previously shown that non-invasive point measurements of absolute PpIX concentration identifies residual tumor that is otherwise non-detectable. Here, we extend this approach to wide-field quantitative fluorescence imaging by implementing spatial frequency domain imaging to recover tissue optical properties across the field-of-view in phantoms and ex vivo tissue. PMID:26713206

Spatially Resolved Quantification of the Surface Reactivity of Solid Catalysts.

PubMed

Huang, Bing; Xiao, Li; Lu, Juntao; Zhuang, Lin

2016-05-17

A new property is reported that accurately quantifies and spatially describes the chemical reactivity of solid surfaces. The core idea is to create a reactivity weight function peaking at the Fermi level, thereby determining a weighted summation of the density of states of a solid surface. When such a weight function is defined as the derivative of the Fermi-Dirac distribution function at a certain non-zero temperature, the resulting property is the finite-temperature chemical softness, termed Fermi softness (SF ), which turns out to be an accurate descriptor of the surface reactivity. The spatial image of SF maps the reactive domain of a heterogeneous surface and even portrays morphological details of the reactive sites. SF analyses reveal that the reactive zones on a Pt3 Y(111) surface are the platinum sites rather than the seemingly active yttrium sites, and the reactivity of the S-dimer edge of MoS2 is spatially anisotropic. Our finding is of fundamental and technological significance to heterogeneous catalysis and industrial processes demanding rational design of solid catalysts. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Estimates of spatially and temporally resolved constrained black carbon emission over the Indian region using a strategic integrated modelling approach

NASA Astrophysics Data System (ADS)

Verma, S.; Reddy, D. Manigopal; Ghosh, S.; Kumar, D. Bharath; Chowdhury, A. Kundu

2017-10-01

We estimated the latest spatially and temporally resolved gridded constrained black carbon (BC) emissions over the Indian region using a strategic integrated modelling approach. This was done extracting information on initial bottom-up emissions and atmospheric BC concentration from a general circulation model (GCM) simulation in conjunction with the receptor modelling approach. Monthly BC emission (83-364 Gg) obtained from the present study exhibited a spatial and temporal variability with this being the highest (lowest) during February (July). Monthly BC emission flux was considerably high (> 100 kg km- 2) over the entire Indo-Gangetic plain (IGP), east and the west coast during winter months. This was relatively higher over the central and western India than over the IGP during summer months. Annual BC emission rate was 2534 Gg y- 1 with that over the IGP and central India respectively comprising 50% and 40% of the total annual BC emissions over India. A high relative increase was observed in modified BC emissions (more than five times the initial emissions) over the most part of the IGP, east coast, central/northwestern India. The relative predominance of monthly BC emission flux over a region (as depicted from z-score distribution maps) was inferred being consistent with the prevalence of region- and season-specific anthropogenic activity.

Final Technical Report - Consolidating Biomass Pretreatment with Saccharification by Resolving the Spatial Control Mechanisms of Fungi

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schilling, Jonathan

Consolidated bioprocessing (CBP) of lignocellulose combines enzymatic sugar release (saccharification) with fermentation, but pretreatments remain separate and costly. In nature, lignocellulose-degrading brown rot fungi consolidate pretreatment and saccharification, likely using spatial gradients to partition these incompatible reactions. With the field of biocatalysis maturing, reaction partitioning is increasingly reproducible for commercial use. Therefore, my goal was to resolve the reaction partitioning mechanisms of brown rot fungi so that they can be applied to bioconversion of lignocellulosic feedstocks. Brown rot fungi consolidate oxidative pretreatments with saccharification and are a focus for biomass refining because 1) they attain >99% sugar yield without destroyingmore » lignin, 2) they use a simplified cellulase suite that lacks exoglucanase, and 3) their non-enzymatic pretreatment is facilitative and may be accelerated. Specifically, I hypothesized that during brown rot, oxidative pretreatments occur ahead of enzymatic saccharification, spatially, and the fungus partitions these reactions using gradients in pH, lignin reactivity, and plant cell wall porosity. In fact, we found three key results during these experiments for this work: 1) Brown rot fungi have an inducible cellulase system, unlike previous descriptions of a constitutive mechanism. 2) The induction of cellulases is delayed until there is repression of oxidatively-linked genes, allowing the brown rot fungi to coordinate two incompatible reactions (oxidative pretreatment with enzymatic saccharification, to release wood sugars) in the same pieces of wood. 3) This transition is mediated by the same wood sugar, cellobiose, released by the oxidative pretreatment step. Collectively, these findings have been published in excellent journal outlets and have been presented at conferences around the United States, and they offer clear targets for gene discovery en route to making biofuels and

Direct observation of single layer graphene oxide reduction through spatially resolved, single sheet absorption/emission microscopy.

PubMed

Sokolov, Denis A; Morozov, Yurii V; McDonald, Matthew P; Vietmeyer, Felix; Hodak, Jose H; Kuno, Masaru

2014-06-11

Laser reduction of graphene oxide (GO) offers unique opportunities for the rapid, nonchemical production of graphene. By tuning relevant reduction parameters, the band gap and conductivity of reduced GO can be precisely controlled. In situ monitoring of single layer GO reduction is therefore essential. In this report, we show the direct observation of laser-induced, single layer GO reduction through correlated changes to its absorption and emission. Absorption/emission movies illustrate the initial stages of single layer GO reduction, its transition to reduced-GO (rGO) as well as its subsequent decomposition upon prolonged laser illumination. These studies reveal GO's photoreduction life cycle and through it native GO/rGO absorption coefficients, their intrasheet distributions as well as their spatial heterogeneities. Extracted absorption coefficients for unreduced GO are α405 nm ≈ 6.5 ± 1.1 × 10(4) cm(-1), α520 nm ≈ 2.1 ± 0.4 × 10(4) cm(-1), and α640 nm ≈ 1.1 ± 0.3 × 10(4) cm(-1) while corresponding rGO α-values are α405 nm ≈ 21.6 ± 0.6 × 10(4) cm(-1), α520 nm ≈ 16.9 ± 0.4 × 10(4) cm(-1), and α640 nm ≈ 14.5 ± 0.4 × 10(4) cm(-1). More importantly, the correlated absorption/emission imaging provides us with unprecedented insight into GO's underlying photoreduction mechanism, given our ability to spatially resolve its kinetics and to connect local rate constants to activation energies. On a broader level, the developed absorption imaging is general and can be applied toward investigating the optical properties of other two-dimensional materials, especially those that are nonemissive and are invisible to current single molecule optical techniques.

A Quantitative Visual Mapping and Visualization Approach for Deep Ocean Floor Research

NASA Astrophysics Data System (ADS)

Hansteen, T. H.; Kwasnitschka, T.

2013-12-01

Geological fieldwork on the sea floor is still impaired by our inability to resolve features on a sub-meter scale resolution in a quantifiable reference frame and over an area large enough to reveal the context of local observations. In order to overcome these issues, we have developed an integrated workflow of visual mapping techniques leading to georeferenced data sets which we examine using state-of-the-art visualization technology to recreate an effective working style of field geology. We demonstrate a microbathymetrical workflow, which is based on photogrammetric reconstruction of ROV imagery referenced to the acoustic vehicle track. The advantage over established acoustical systems lies in the true three-dimensionality of the data as opposed to the perspective projection from above produced by downward looking mapping methods. A full color texture mosaic derived from the imagery allows studies at resolutions beyond the resolved geometry (usually one order of magnitude below the image resolution) while color gives additional clues, which can only be partly resolved in acoustic backscatter. The creation of a three-dimensional model changes the working style from the temporal domain of a video recording back to the spatial domain of a map. We examine these datasets using a custom developed immersive virtual visualization environment. The ARENA (Artificial Research Environment for Networked Analysis) features a (lower) hemispherical screen at a diameter of six meters, accommodating up to four scientists at once thus providing the ability to browse data interactively among a group of researchers. This environment facilitates (1) the development of spatial understanding analogue to on-land outcrop studies, (2) quantitative observations of seafloor morphology and physical parameters of its deposits, (3) more effective formulation and communication of working hypotheses.

Spatially resolved nuclear spin relaxation, electron spin relaxation and light absorption in swift heavy ion irradiated LiF crystals.

PubMed

Stork, H; Dinse, K-P; Ditter, M; Fujara, F; Masierak, W; Neumann, R; Schuster, B; Schwartz, K; Trautmann, C

2010-05-12

Spatially resolved (19)F and (7)Li spin-lattice relaxation rates are measured for LiF single crystals after irradiation with two kinds of swift heavy ions ((12)C of 133 MeV and (208)Pb of 1.78 GeV incident energy). Like in earlier studies on (130)Xe and (238)U irradiated LiF crystals, we found a strong enhancement of the nuclear spin-lattice relaxation rate within the ion penetration depth and a slight--but still significant--enhancement beyond. By evaluating the nuclear relaxation rate enhancement within the ion range after irradiation with different projectiles, a universal relationship between the spin-lattice relaxation rate and the dose is deduced. The results of accompanying X-band electron paramagnetic resonance relaxation measurements and optical absorption spectroscopy are included in a physical interpretation of this relationship. Also the reason for the enhanced relaxation rate beyond the ion range is further discussed.

Solvent and solute ingress into hydrogels resolved by a combination of imaging techniques

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wagner, D.; Burbach, J.; Egelhaaf, S. U.

2016-05-28

Using simultaneous neutron, fluorescence, and optical brightfield transmission imaging, the diffusion of solvent, fluorescent dyes, and macromolecules into a crosslinked polyacrylamide hydrogel was investigated. This novel combination of different imaging techniques enables us to distinguish the movements of the solvent and fluorescent molecules. Additionally, the swelling or deswelling of the hydrogels can be monitored. From the sequence of images, dye and solvent concentrations were extracted spatially and temporally resolved. Diffusion equations and different boundary conditions, represented by different models, were used to quantitatively analyze the temporal evolution of these concentration profiles and to determine the diffusion coefficients of solvent andmore » solutes. Solute size and network properties were varied and their effect was investigated. Increasing the crosslinking ratio or partially drying the hydrogel was found to hinder solute diffusion due to the reduced pore size. By contrast, solvent diffusion seemed to be slightly faster if the hydrogel was only partially swollen and hence solvent uptake enhanced.« less

Covariance J-resolved spectroscopy: Theory and application in vivo.

PubMed

Iqbal, Zohaib; Verma, Gaurav; Kumar, Anand; Thomas, M Albert

2017-08-01

Magnetic resonance spectroscopy (MRS) is a powerful tool capable of investigating the metabolic status of several tissues in vivo. In particular, single-voxel-based 1 H spectroscopy provides invaluable biochemical information from a volume of interest (VOI) and has therefore been used in a variety of studies. Unfortunately, typical one-dimensional MRS data suffer from severe signal overlap and thus important metabolites are difficult to distinguish. One method that is used to disentangle overlapping resonances is the two-dimensional J-resolved spectroscopy (JPRESS) experiment. Due to the long acquisition duration of the JPRESS experiment, a limited number of points are acquired in the indirect dimension, leading to poor spectral resolution along this dimension. Poor spectral resolution is problematic because proper peak assignment may be hindered, which is why the zero-filling method is often used to improve resolution as a post-processing step. However, zero-filling leads to spectral artifacts, which may affect visualization and quantitation of spectra. A novel method utilizing a covariance transformation, called covariance J-resolved spectroscopy (CovJ), was developed in order to improve spectral resolution along the indirect dimension (F 1 ). Comparison of simulated data demonstrates that peak structures remain qualitatively similar between JPRESS and the novel method along the diagonal region (F 1 = 0 Hz), whereas differences arise in the cross-peak (F 1 ≠0 Hz) regions. In addition, quantitative results of in vivo JPRESS data acquired on a 3T scanner show significant correlations (r 2 >0.86, p<0.001) when comparing the metabolite concentrations between the two methods. Finally, a quantitation algorithm, 'COVariance Spectral Evaluation of 1 H Acquisitions using Representative prior knowledge' (Cov-SEHAR), was developed in order to quantify γ-aminobutyric acid and glutamate from the CovJ spectra. These preliminary findings indicate that the CovJ method may

Quantifying the correlation between spatially defined oxygen gradients and cell fate in an engineered three-dimensional culture model.

PubMed

Ardakani, Amir G; Cheema, Umber; Brown, Robert A; Shipley, Rebecca J

2014-09-06

A challenge in three-dimensional tissue culture remains the lack of quantitative information linking nutrient delivery and cellular distribution. Both in vivo and in vitro, oxygen is delivered by diffusion from its source (blood vessel or the construct margins). The oxygen level at a defined distance from its source depends critically on the balance of diffusion and cellular metabolism. Cells may respond to this oxygen environment through proliferation, death and chemotaxis, resulting in spatially resolved gradients in cellular density. This study extracts novel spatially resolved and simultaneous data on tissue oxygenation, cellular proliferation, viability and chemotaxis in three-dimensional spiralled, cellular collagen constructs. Oxygen concentration gradients drove preferential cellular proliferation rates and viability in the higher oxygen zones and induced chemotaxis along the spiral of the collagen construct; an oxygen gradient of 1.03 mmHg mm(-1) in the spiral direction induced a mean migratory speed of 1015 μm day(-1). Although this movement was modest, it was effective in balancing the system to a stable cell density distribution, and provided insights into the natural cell mechanism for adapting cell number and activity to a prevailing oxygen regime.

Quantitative Measurements of Nitric Oxide Concentration in High-Pressure, Swirl-Stabilized Spray Flames

NASA Technical Reports Server (NTRS)

Cooper, Clayton S.; Laurendeau, Normand M.; Hicks, Yolanda R. (Technical Monitor)

2000-01-01

Lean direct-injection (LDI) spray flames offer the possibility of reducing NO(sub x) emissions from gas turbines by rapid mixing of the liquid fuel and air so as to drive the flame structure toward partially-premixed conditions. We consider the technical approaches required to utilize laser-induced fluorescence methods for quantitatively measuring NO concentrations in high-pressure LDI spray flames. In the progression from atmospheric to high-pressure measurements, the LIF method requires a shift from the saturated to the linear regime of fluorescence measurements. As such, we discuss quantitative, spatially resolved laser-saturated fluorescence (LSF), linear laser-induced fluorescence (LIF), and planar laser-induced fluorescence (PLIF) measurements of NO concentration in LDI spray flames. Spatially-resolved LIF measurements of NO concentration (ppm) are reported for preheated, LDI spray flames at pressures of two to five atmospheres. The spray is produced by a hollow-cone, pressure-atomized nozzle supplied with liquid heptane. NO is excited via the Q(sub 2)(26.5) transition of the gamma(0,0) band. Detection is performed in a two nanometer region centered on the gamma(0,1) band. A complete scheme is developed by which quantitative NO concentrations in high-pressure LDI spray flames can be measured by applying linear LIF. NO is doped into the reactants and convected through the flame with no apparent destruction, thus allowing a NO fluorescence calibration to be taken inside the flame environment. The in-situ calibration scheme is validated by comparisons to a reference flame. Quantitative NO profiles are presented and analyzed so as to better understand the operation of lean-direct injectors for gas turbine combustors. Moreover, parametric studies are provided for variations in pressure, air-preheat temperature, and equivalence ratio. Similar parametric studies are performed for lean, premixed-prevaporized flames to permit comparisons to those for LDI flames

Resolving z ~2 galaxy using adaptive coadded source plane reconstruction

NASA Astrophysics Data System (ADS)

Sharma, Soniya; Richard, Johan; Kewley, Lisa; Yuan, Tiantian

2018-06-01

Natural magnification provided by gravitational lensing coupled with Integral field spectrographic observations (IFS) and adaptive optics (AO) imaging techniques have become the frontier of spatially resolved studies of high redshift galaxies (z>1). Mass models of gravitational lenses hold the key for understanding the spatially resolved source–plane (unlensed) physical properties of the background lensed galaxies. Lensing mass models very sensitively control the accuracy and precision of source-plane reconstructions of the observed lensed arcs. Effective source-plane resolution defined by image-plane (observed) point spread function (PSF) makes it challenging to recover the unlensed (source-plane) surface brightness distribution.We conduct a detailed study to recover the source-plane physical properties of z=2 lensed galaxy using spatially resolved observations from two different multiple images of the lensed target. To deal with PSF’s from two data sets on different multiple images of the galaxy, we employ a forward (Source to Image) approach to merge these independent observations. Using our novel technique, we are able to present a detailed analysis of the source-plane dynamics at scales much better than previously attainable through traditional image inversion methods. Moreover, our technique is adapted to magnification, thus allowing us to achieve higher resolution in highly magnified regions of the source. We find that this lensed system is highly evident of a minor merger. In my talk, I present this case study of z=2 lensed galaxy and also discuss the applications of our algorithm to study plethora of lensed systems, which will be available through future telescopes like JWST and GMT.

The Spatially Resolved H(alpha)-Emitting Wind Structure of P Cygni

DTIC Science & Technology

2010-06-01

using radio and optical interferometry, as well as direct imaging with adaptive optics (AO). Radio interferometric observations detect the nebula around...to structures in the nebula of P Cyg that are more than an order of magnitude smaller. Therefore, optical interferometry provides a unique window of...resolved the outer Hα-emitting region of the extended envelope, but detected signatures of clumping. Although, the angular scales sampled with a 1.52 m

Lateral Asymmetry and Spatial Difference of Iron Deposition in the Substantia Nigra of Patients with Parkinson Disease Measured with Quantitative Susceptibility Mapping.

PubMed

Azuma, M; Hirai, T; Yamada, K; Yamashita, S; Ando, Y; Tateishi, M; Iryo, Y; Yoneda, T; Kitajima, M; Wang, Y; Yamashita, Y

2016-05-01

Quantitative susceptibility mapping is useful for assessing iron deposition in the substantia nigra of patients with Parkinson disease. We aimed to determine whether quantitative susceptibility mapping is useful for assessing the lateral asymmetry and spatial difference in iron deposits in the substantia nigra of patients with Parkinson disease. Our study population comprised 24 patients with Parkinson disease and 24 age- and sex-matched healthy controls. They underwent 3T MR imaging by using a 3D multiecho gradient-echo sequence. On reconstructed quantitative susceptibility mapping, we measured the susceptibility values in the anterior, middle, and posterior parts of the substantia nigra, the whole substantia nigra, and other deep gray matter structures in both hemibrains. To identify the more and less affected hemibrains in patients with Parkinson disease, we assessed the severity of movement symptoms for each hemibrain by using the Unified Parkinson's Disease Rating Scale. In the posterior substantia nigra of patients with Parkinson disease, the mean susceptibility value was significantly higher in the more than the less affected hemibrain substantia nigra (P < .05). This value was significantly higher in both the more and less affected hemibrains of patients with Parkinson disease than in controls (P < .05). Asymmetry of the mean susceptibility values was significantly greater for patients than controls (P < .05). Receiver operating characteristic analysis showed that quantitative susceptibility mapping of the posterior substantia nigra in the more affected hemibrain provided the highest power for discriminating patients with Parkinson disease from the controls. Quantitative susceptibility mapping is useful for assessing the lateral asymmetry and spatial difference of iron deposition in the substantia nigra of patients with Parkinson disease. © 2016 by American Journal of Neuroradiology.

Spatially resolved speckle-correlometry of sol-gel transition

NASA Astrophysics Data System (ADS)

Isaeva, A. A.; Isaeva, E. A.; Pantyukov, A. V.; Zimnyakov, D. A.

2018-04-01

Sol-gel transition was studied using the speckle correlometry method with a localized light source and spatial filtering of backscattered radiation. Water solutions of technical or food gelatin with added TiO2 nanoparticles were used as studied objects. Structural transformation of "sol-gel" system was studied at various temperatures from 25°C to 50°C using analysis of the correlation and structure functions of speckle intensity fluctuations. The characteristic temperatures of "sol - gel" transition were evaluated for studied systems. Obtained results can be used for various applications in biomedicine and food industry.

Time-resolved measurements of supersonic fuel sprays using synchrotron X-rays.

PubMed

Powell, C F; Yue, Y; Poola, R; Wang, J

2000-11-01

A time-resolved radiographic technique has been developed for probing the fuel distribution close to the nozzle of a high-pressure single-hole diesel injector. The measurement was made using X-ray absorption of monochromatic synchrotron-generated radiation, allowing quantitative determination of the fuel distribution in this optically impenetrable region with a time resolution of better than 1 micros. These quantitative measurements constitute the most detailed near-nozzle study of a fuel spray to date.

Phase-resolved acoustic radiation force optical coherence elastography

NASA Astrophysics Data System (ADS)

Qi, Wenjuan; Chen, Ruimin; Chou, Lidek; Liu, Gangjun; Zhang, Jun; Zhou, Qifa; Chen, Zhongping

2012-11-01

Many diseases involve changes in the biomechanical properties of tissue, and there is a close correlation between tissue elasticity and pathology. We report on the development of a phase-resolved acoustic radiation force optical coherence elastography method (ARF-OCE) to evaluate the elastic properties of tissue. This method utilizes chirped acoustic radiation force to produce excitation along the sample's axial direction, and it uses phase-resolved optical coherence tomography (OCT) to measure the vibration of the sample. Under 500-Hz square wave modulated ARF signal excitation, phase change maps of tissue mimicking phantoms are generated by the ARF-OCE method, and the resulting Young's modulus ratio is correlated with a standard compression test. The results verify that this technique could efficiently measure sample elastic properties accurately and quantitatively. Furthermore, a three-dimensional ARF-OCE image of the human atherosclerotic coronary artery is obtained. The result indicates that our dynamic phase-resolved ARF-OCE method can delineate tissues with different mechanical properties.

Spatially resolved wavefront aberrations of ophthalmic progressive-power lenses in normal viewing conditions.

PubMed

Villegas, Eloy A; Artal, Pablo

2003-02-01

To measure the wavefront aberration at different locations in progressive-power lenses (PPL's) isolated and in situ (PPL's plus eye). A Hartmann-Shack wavefront sensor was used to measure progressive-power lenses and human eyes either independently or in combination. In each selected zone, the lens was placed and tilted accordingly to simulate natural viewing conditions. We measured 21 relevant locations across an isolated PPL (plano lens of power addition of 2 D). In six of the locations, the wavefront aberration of the eye plus PPL were obtained in two ways: (1) by direct measurement of the system and (2) by adding the individual wavefront aberrations of the eye and the lens for each appropriate zone. In every case, we obtained the wavefront aberration as Zernike polynomials expansions, the root mean square error, the point-spread function, and the Strehl ratio. Along the corridor of the PPL, third-order coma and trefoil, and astigmatism were the dominant aberrations. In areas of the PPL outside the corridor, astigmatism increased, whereas other aberrations remained similar to the lens center. Small differences were found between the direct and calculated methods used to obtain the wavefront aberration of the eye with the lens, and the possible sources of errors were discussed. In some lenses zones, the aberrations of the lens may be compensated by the particular aberrations of the eye, yielding improved optical performance over that present in the lens alone. We designed and built a wavefront sensor to perform spatially resolved aberration measurements in ophthalmic lenses, in particular in PPL's, either isolated or in combination with the eye. The aberrations appearing in the PPL were compared with those in normal aged eyes.

Super-resolved refocusing with a plenoptic camera

NASA Astrophysics Data System (ADS)

Zhou, Zhiliang; Yuan, Yan; Bin, Xiangli; Qian, Lulu

2011-03-01

This paper presents an approach to enhance the resolution of refocused images by super resolution methods. In plenoptic imaging, we demonstrate that the raw sensor image can be divided to a number of low-resolution angular images with sub-pixel shifts between each other. The sub-pixel shift, which defines the super-resolving ability, is mathematically derived by considering the plenoptic camera as equivalent camera arrays. We implement simulation to demonstrate the imaging process of a plenoptic camera. A high-resolution image is then reconstructed using maximum a posteriori (MAP) super resolution algorithms. Without other degradation effects in simulation, the super resolved image achieves a resolution as high as predicted by the proposed model. We also build an experimental setup to acquire light fields. With traditional refocusing methods, the image is rendered at a rather low resolution. In contrast, we implement the super-resolved refocusing methods and recover an image with more spatial details. To evaluate the performance of the proposed method, we finally compare the reconstructed images using image quality metrics like peak signal to noise ratio (PSNR).

Toward reliable retrieval of functional information of papillary dermis using spatially resolved diffuse reflectance spectroscopy

PubMed Central

Chen, Yu-Wen; Guo, Jun-Yen; Tzeng, Shih-Yu; Chou, Ting-Chun; Lin, Ming-Jen; Huang, Lynn Ling-Huei; Yang, Chao-Chun; Hsu, Chao-Kai; Tseng, Sheng-Hao

2016-01-01

Spatially resolved diffuse reflectance spectroscopy (SRDRS) has been employed to quantify tissue optical properties and its interrogation volume is majorly controlled by the source-to-detector separations (SDSs). To noninvasively quantify properties of dermis, a SRDRS setup that includes SDS shorter than 1 mm is required. It will be demonstrated in this study that Monte Carlo simulations employing the Henyey-Greenstein phase function cannot always precisely predict experimentally measured diffuse reflectance at such short SDSs, and we speculated this could be caused by the non-negligible backward light scattering at short SDSs that cannot be properly modeled by the Henyey-Greenstein phase function. To accurately recover the optical properties and functional information of dermis using SRDRS, we proposed the use of the modified two-layer (MTL) geometry. Monte Carlo simulations and phantom experiment results revealed that the MTL probing geometry was capable of faithfully recovering the optical properties of upper dermis. The capability of the MTL geometry in probing the upper dermis properties was further verified through a swine study, and it was found that the measurement results were reasonably linked to histological findings. Finally, the MTL probe was utilized to study psoriatic lesions. Our results showed that the MTL probe was sensitive to the physiological condition of tissue volumes within the papillary dermis and could be used in studying the physiology of psoriasis. PMID:26977361

Toward reliable retrieval of functional information of papillary dermis using spatially resolved diffuse reflectance spectroscopy.

PubMed

Chen, Yu-Wen; Guo, Jun-Yen; Tzeng, Shih-Yu; Chou, Ting-Chun; Lin, Ming-Jen; Huang, Lynn Ling-Huei; Yang, Chao-Chun; Hsu, Chao-Kai; Tseng, Sheng-Hao

2016-02-01

Spatially resolved diffuse reflectance spectroscopy (SRDRS) has been employed to quantify tissue optical properties and its interrogation volume is majorly controlled by the source-to-detector separations (SDSs). To noninvasively quantify properties of dermis, a SRDRS setup that includes SDS shorter than 1 mm is required. It will be demonstrated in this study that Monte Carlo simulations employing the Henyey-Greenstein phase function cannot always precisely predict experimentally measured diffuse reflectance at such short SDSs, and we speculated this could be caused by the non-negligible backward light scattering at short SDSs that cannot be properly modeled by the Henyey-Greenstein phase function. To accurately recover the optical properties and functional information of dermis using SRDRS, we proposed the use of the modified two-layer (MTL) geometry. Monte Carlo simulations and phantom experiment results revealed that the MTL probing geometry was capable of faithfully recovering the optical properties of upper dermis. The capability of the MTL geometry in probing the upper dermis properties was further verified through a swine study, and it was found that the measurement results were reasonably linked to histological findings. Finally, the MTL probe was utilized to study psoriatic lesions. Our results showed that the MTL probe was sensitive to the physiological condition of tissue volumes within the papillary dermis and could be used in studying the physiology of psoriasis.

The importance of spatial heterogeneity and self-restraint on mutualism stability - a quantitative review

NASA Astrophysics Data System (ADS)

Wang, Rui-Wu; Dunn, Derek W.; Luo, Jun; He, Jun-Zhou; Shi, Lei

2015-10-01

Understanding the factors that enable mutualisms to evolve and to subsequently remain stable over time, is essential to fully understand patterns of global biodiversity and for evidence based conservation policy. Theoretically, spatial heterogeneity of mutualists, through increased likelihood of fidelity between cooperative partners in structured populations, and ‘self-restraint’ of symbionts, due to selection against high levels of virulence leading to short-term host overexploitation, will result in either a positive correlation between the reproductive success of both mutualists prior to the total exploitation of any host resource or no correlation after any host resource has been fully exploited. A quantitative review by meta-analysis on the results of 96 studies from 35 papers, showed no evidence of a significant fitness correlation between mutualists across a range of systems that captured much taxonomic diversity. However, when the data were split according to four categories of host: 1) cnidarian corals, 2) woody plants, 3) herbaceous plants, and 4) insects, a significantly positive effect in corals was revealed. The trends for the remaining three categories did not significantly differ to zero. Our results suggest that stability in mutualisms requires alternative processes, or mechanisms in addition to, spatial heterogeneity of hosts and/or ‘self-restraint’ of symbionts.

High sensitive and high temporal and spatial resolved image of reactive species in atmospheric pressure surface discharge reactor by laser induced fluorescence

NASA Astrophysics Data System (ADS)

Gao, Liang; Feng, Chun-Lei; Wang, Zhi-Wei; Ding, Hongbin

2017-05-01

The current paucity of spatial and temporal characterization of reactive oxygen and nitrogen species (RONS) concentration has been a major hurdle to the advancement and clinical translation of low temperature atmospheric plasmas. In this study, an advanced laser induced fluorescence (LIF) system has been developed to be an effective antibacterial surface discharge reactor for the diagnosis of RONS, where the highest spatial and temporal resolution of the LIF system has been achieved to ˜100 μm scale and ˜20 ns scale, respectively. Measurements on an oxidative OH radical have been carried out as typical RONS for the benchmark of the whole LIF system, where absolute number density calibration has been performed on the basis of the laser Rayleigh scattering method. Requirements for pixel resolved spatial distribution and outer plasma region detection become challenging tasks due to the low RONS concentration (˜ppb level) and strong interference, especially the discharge induced emission and pulsed laser induced stray light. In order to design the highly sensitive LIF system, a self-developed fluorescence telescope, the optimization of high precision synchronization among a tunable pulsed laser, a surface discharge generator, intensified Charge Coupled Device (iCCD) camera, and an oscilloscope have been performed. Moreover, an image BOXCAR approach has been developed to remarkably improve the sensitivity of the whole LIF system by optimizing spatial and temporal gating functions via both hardware and software, which has been integrated into our automatic control and data acquisition system on the LabVIEW platform. In addition, a reciprocation averaging measurement has been applied to verify the accuracy of the whole LIF detecting system, indicating the relative standard deviation of ˜3%.

Early Development of Spatial-Numeric Associations: Evidence from Spatial and Quantitative Performance of Preschoolers

ERIC Educational Resources Information Center

Opfer, John E.; Thompson, Clarissa A.; Furlong, Ellen E.

2010-01-01

Numeric magnitudes often bias adults' spatial performance. Partly because the direction of this bias (left-to-right versus right-to-left) is culture-specific, it has been assumed that the orientation of spatial-numeric associations is a late development, tied to reading practice or schooling. Challenging this assumption, we found that preschoolers…

Spatial-spectral characterization of focused spatially chirped broadband laser beams.

PubMed

Greco, Michael J; Block, Erica; Meier, Amanda K; Beaman, Alex; Cooper, Samuel; Iliev, Marin; Squier, Jeff A; Durfee, Charles G

2015-11-20

Proper alignment is critical to obtain the desired performance from focused spatially chirped beams, for example in simultaneous spatial and temporal focusing (SSTF). We present a simple technique for inspecting the beam paths and focusing conditions for the spectral components of a broadband beam. We spectrally resolve the light transmitted past a knife edge as it was scanned across the beam at several axial positions. The measurement yields information about spot size, M2, and the propagation paths of different frequency components. We also present calculations to illustrate the effects of defocus aberration on SSTF beams.

Spatially quantitative seafloor habitat mapping: Example from the northern South Carolina inner continental shelf

USGS Publications Warehouse

Ojeda, G.Y.; Gayes, P.T.; Van Dolah, R. F.; Schwab, W.C.

2004-01-01

Naturally occurring hard bottom areas provide the geological substrate that can support diverse assemblages of sessile benthic organisms, which in turn, attract many reef-dwelling fish species. Alternatively, defining the location and extent of bottom sand bodies is relevant for potential nourishment projects as well as to ensure that transient sediment does not affect reef habitats, particularly in sediment-starved continental margins. Furthermore, defining sediment transport pathways documents the effects these mobile bedforms have on proximal reef habitats. Thematic mapping of these substrates is therefore crucial in safeguarding critical habitats and offshore resources of coastal nations. This study presents the results of a spatially quantitative mapping approach based on classification of sidescan-sonar imagery. By using bottom video for image-to-ground control, digital image textural features for pattern recognition, and an artificial neural network for rapid, quantitative, multivariable decision-making, this approach resulted in recognition rates of hard bottom as high as 87%. The recognition of sand bottom was less successful (31%). This approach was applied to a large (686 km2), high-quality, 2-m resolution sidescan-sonar mosaic of the northern South Carolina inner continental shelf. Results of this analysis indicate that both surficial sand and hard bottoms of variable extent are present over the study area. In total, 59% of the imaged area was covered by hard bottom, while 41% was covered by sand. Qualitative spatial correlation between bottom type and bathymetry appears possible from comparison of our interpretive map and available bathymetry. Hard bottom areas tend to be located on flat, low-lying areas, and sandy bottoms tend to reside on areas of positive relief. Published bio-erosion rates were used to calculate the potential sediment input from the mapped hard bottom areas rendering sediment volumes that may be as high as 0.8 million m3/yr for

Estimation of the absorption coefficients of two-layered media by a simple method using spatially and time-resolved reflectances

NASA Astrophysics Data System (ADS)

Shimada, M.; Sato, C.; Hoshi, Y.; Yamada, Y.

2009-08-01

Our newly developed method using spatially and time-resolved reflectances can easily estimate the absorption coefficients of each layer in a two-layered medium if the thickness of the upper layer and the reduced scattering coefficients of the two layers are known a priori. We experimentally validated this method using phantoms and examined its possibility of estimating the absorption coefficients of the tissues in human heads. In the case of a homogeneous plastic phantom (polyacetal block), the absorption coefficient estimated by our method agreed well with that obtained by a conventional method. Also, in the case of two-layered phantoms, our method successfully estimated the absorption coefficients of the two layers. Furthermore, the absorption coefficients of the extracerebral and cerebral tissue inside human foreheads were estimated under the assumption that the human heads were two-layered media. It was found that the absorption coefficients of the cerebral tissues were larger than those of the extracerebral tissues.

Passive Standoff Super Resolution Imaging using Spatial-Spectral Multiplexing

DTIC Science & Technology

2017-08-14

94 5.0 Four -Dimensional Object-Space Data Reconstruction Using Spatial...103 5.3 Four -dimensional scene reconstruction using SSM...transitioning to systems based on spectrally resolved longitudinal spatial coherence interferometry. This document also includes research related to four

Spatially Resolved MR-Compatible Doppler Ultrasound: Proof of Concept for Triggering of Diagnostic Quality Cardiovascular MRI for Function and Flow Quantification at 3T.

PubMed

Crowe, Lindsey Alexandra; Manasseh, Gibran; Chmielewski, Aneta; Hachulla, Anne-Lise; Speicher, Daniel; Greiser, Andreas; Muller, Hajo; de Perrot, Thomas; Vallee, Jean-Paul; Salomir, Rares

2018-02-01

We demonstrate the use of a magnetic-resonance (MR)-compatible ultrasound (US) imaging probe using spatially resolved Doppler for diagnostic quality cardiovascular MR imaging (MRI) as an initial step toward hybrid US/MR fetal imaging. A newly developed technology for a dedicated MR-compatible phased array ultrasound-imaging probe acquired pulsed color Doppler carotid images, which were converted in near-real time to a trigger signal for cardiac cine and flow quantification MRI. Ultrasound and MR data acquired simultaneously were interference free. Conventional electrocardiogram (ECG) and the proposed spatially resolved Doppler triggering were compared in 10 healthy volunteers. A synthetic "false-triggered" image was retrospectively processed using metric optimized gating (MOG). Images were scored by expert readers, and sharpness, cardiac function and aortic flow were quantified. Four-dimensional (4-D) flow (two volunteers) showed feasibility of Doppler triggering over a long acquisition time. Imaging modalities were compatible. US probe positioning was stable and comfortable. Image quality scores and quantified sharpness were statistically equal for Doppler- and ECG-triggering (p ). ECG-, Doppler-triggered, and MOG ejection fractions were equivalent (p ), with false-triggered values significantly lower (p < 0.0005). Aortic flow showed no difference between ECG- and Doppler-triggered and MOG (p > 0.05). 4-D flow quantification gave consistent results between ECG and Doppler triggering. We report interference-free pulsed color Doppler ultrasound during MR data acquisition. Cardiovascular MRI of diagnostic quality was successfully obtained with pulsed color Doppler triggering. The hardware platform could further enable advanced free-breathing cardiac imaging. Doppler ultrasound triggering is applicable where ECG is compromised due to pathology or interference at higher magnetic fields, and where direct ECG is impossible, i.e., fetal imaging.

Early development of spatial-numeric associations: evidence from spatial and quantitative performance of preschoolers.

PubMed

Opfer, John E; Thompson, Clarissa A; Furlong, Ellen E

2010-09-01

Numeric magnitudes often bias adults' spatial performance. Partly because the direction of this bias (left-to-right versus right-to-left) is culture-specific, it has been assumed that the orientation of spatial-numeric associations is a late development, tied to reading practice or schooling. Challenging this assumption, we found that preschoolers expected numbers to be ordered from left-to-right when they searched for objects in numbered containers, when they counted, and (to a lesser extent) when they added and subtracted. Further, preschoolers who lacked these biases demonstrated more immature, logarithmic representations of numeric value than preschoolers who exhibited the directional bias, suggesting that spatial-numeric associations aid magnitude representations for symbols denoting increasingly large numbers.

A multiscale computational model of spatially resolved calcium cycling in cardiac myocytes: from detailed cleft dynamics to the whole cell concentration profiles

PubMed Central

Vierheller, Janine; Neubert, Wilhelm; Falcke, Martin; Gilbert, Stephen H.; Chamakuri, Nagaiah

2015-01-01

Mathematical modeling of excitation-contraction coupling (ECC) in ventricular cardiac myocytes is a multiscale problem, and it is therefore difficult to develop spatially detailed simulation tools. ECC involves gradients on the length scale of 100 nm in dyadic spaces and concentration profiles along the 100 μm of the whole cell, as well as the sub-millisecond time scale of local concentration changes and the change of lumenal Ca2+ content within tens of seconds. Our concept for a multiscale mathematical model of Ca2+ -induced Ca2+ release (CICR) and whole cardiomyocyte electrophysiology incorporates stochastic simulation of individual LC- and RyR-channels, spatially detailed concentration dynamics in dyadic clefts, rabbit membrane potential dynamics, and a system of partial differential equations for myoplasmic and lumenal free Ca2+ and Ca2+-binding molecules in the bulk of the cell. We developed a novel computational approach to resolve the concentration gradients from dyadic space to cell level by using a quasistatic approximation within the dyad and finite element methods for integrating the partial differential equations. We show whole cell Ca2+-concentration profiles using three previously published RyR-channel Markov schemes. PMID:26441674

Spatially Resolved Spectroscopy of the SNR IC443

NASA Astrophysics Data System (ADS)

Gorenstein, P.

1998-07-01

investigators examined the spatial structure of the thermal component and analyzed the GIS spectra with a non-equilibrium plasma model, and found no systematic variation of the interstellar absorption across the remnant. Evidence for shock acceleration of cosmic rays to high energies (10 TeV) was found by Keohane. X-ray imaging spectroscopy with ASCA reveals two regions of particularly hard emission: an unresolved source embedded in an extended emission region, and a ridge of emission coincident with the southeastern rim. Both features are located on part of the radio shell where the shock wave is interacting with molecular gas, and together they account for a majority of the emission at 7 keV. Though we would not have noticed it a priori, the unresolved feature is coincident with one resolved by the ROSAT HRI. The ASCA measurements were combined with higher energy data from the XTE and GRO missions and with radio and TeV gamma-ray data to produce a nonthermal multiwavelength spectrum for IC 443 which was fit with a cosmic ray interaction model. This model calculates the cynchrotron, bremsstrahlung, invers Compton, and neutral pion decay emission produced by locally accelerated cosmic ray interacting with ambient matter, soft photon fields, and magnetic fields.

Spatially Resolved Spectroscopy of the SNR IC443

NASA Technical Reports Server (NTRS)

Gorenstein, P.

1998-01-01

investigators examined the spatial structure of the thermal component and analyzed the GIS spectra with a non-equilibrium plasma model, and found no systematic variation of the interstellar absorption across the remnant. Evidence for shock acceleration of cosmic rays to high energies (10 TeV) was found by Keohane. X-ray imaging spectroscopy with ASCA reveals two regions of particularly hard emission: an unresolved source embedded in an extended emission region, and a ridge of emission coincident with the southeastern rim. Both features are located on part of the radio shell where the shock wave is interacting with molecular gas, and together they account for a majority of the emission at 7 keV. Though we would not have noticed it a priori, the unresolved feature is coincident with one resolved by the ROSAT HRI. The ASCA measurements were combined with higher energy data from the XTE and GRO missions and with radio and TeV gamma-ray data to produce a nonthermal multiwavelength spectrum for IC 443 which was fit with a cosmic ray interaction model. This model calculates the cynchrotron, bremsstrahlung, invers Compton, and neutral pion decay emission produced by locally accelerated cosmic ray interacting with ambient matter, soft photon fields, and magnetic fields.

User-based representation of time-resolved multimodal public transportation networks.

PubMed

Alessandretti, Laura; Karsai, Márton; Gauvin, Laetitia

2016-07-01

Multimodal transportation systems, with several coexisting services like bus, tram and metro, can be represented as time-resolved multilayer networks where the different transportation modes connecting the same set of nodes are associated with distinct network layers. Their quantitative description became possible recently due to openly accessible datasets describing the geo-localized transportation dynamics of large urban areas. Advancements call for novel analytics, which combines earlier established methods and exploits the inherent complexity of the data. Here, we provide a novel user-based representation of public transportation systems, which combines representations, accounting for the presence of multiple lines and reducing the effect of spatial embeddedness, while considering the total travel time, its variability across the schedule, and taking into account the number of transfers necessary. After the adjustment of earlier techniques to the novel representation framework, we analyse the public transportation systems of several French municipal areas and identify hidden patterns of privileged connections. Furthermore, we study their efficiency as compared to the commuting flow. The proposed representation could help to enhance resilience of local transportation systems to provide better design policies for future developments.

User-based representation of time-resolved multimodal public transportation networks

PubMed Central

Alessandretti, Laura; Gauvin, Laetitia

2016-01-01

Multimodal transportation systems, with several coexisting services like bus, tram and metro, can be represented as time-resolved multilayer networks where the different transportation modes connecting the same set of nodes are associated with distinct network layers. Their quantitative description became possible recently due to openly accessible datasets describing the geo-localized transportation dynamics of large urban areas. Advancements call for novel analytics, which combines earlier established methods and exploits the inherent complexity of the data. Here, we provide a novel user-based representation of public transportation systems, which combines representations, accounting for the presence of multiple lines and reducing the effect of spatial embeddedness, while considering the total travel time, its variability across the schedule, and taking into account the number of transfers necessary. After the adjustment of earlier techniques to the novel representation framework, we analyse the public transportation systems of several French municipal areas and identify hidden patterns of privileged connections. Furthermore, we study their efficiency as compared to the commuting flow. The proposed representation could help to enhance resilience of local transportation systems to provide better design policies for future developments. PMID:27493773

The spatially resolved characterisation of Egyptian blue, Han blue and Han purple by photo-induced luminescence digital imaging.

PubMed

Verri, G

2009-06-01

The photo-induced luminescence properties of Egyptian blue, Han blue and Han purple were investigated by means of near-infrared digital imaging. These pigments emit infrared radiation when excited in the visible range. The emission can be recorded by means of a modified commercial digital camera equipped with suitable glass filters. A variety of visible light sources were investigated to test their ability to excite luminescence in the pigments. Light-emitting diodes, which do not emit stray infrared radiation, proved an excellent source for the excitation of luminescence in all three compounds. In general, the use of visible radiation emitters with low emission in the infrared range allowed the presence of the pigments to be determined and their distribution to be spatially resolved. This qualitative imaging technique can be easily applied in situ for a rapid characterisation of materials. The results were compared to those for Egyptian green and for historical and modern blue pigments. Examples of the application of the technique on polychrome works of art are presented.

Spatially resolved resonant tunneling on single atoms in silicon.

PubMed

Voisin, B; Salfi, J; Bocquel, J; Rahman, R; Rogge, S

2015-04-22

The ability to control single dopants in solid-state devices has opened the way towards reliable quantum computation schemes. In this perspective it is essential to understand the impact of interfaces and electric fields, inherent to address coherent electronic manipulation, on the dopants atomic scale properties. This requires both fine energetic and spatial resolution of the energy spectrum and wave-function, respectively. Here we present an experiment fulfilling both conditions: we perform transport on single donors in silicon close to a vacuum interface using a scanning tunneling microscope (STM) in the single electron tunneling regime. The spatial degrees of freedom of the STM tip provide a versatility allowing a unique understanding of electrostatics. We obtain the absolute energy scale from the thermal broadening of the resonant peaks, allowing us to deduce the charging energies of the donors. Finally we use a rate equations model to derive the current in presence of an excited state, highlighting the benefits of the highly tunable vacuum tunnel rates which should be exploited in further experiments. This work provides a general framework to investigate dopant-based systems at the atomic scale.

Spatially resolved medium resolution spectroscopy of an interacting E+A (post-starburst) system with the Subaru Telescope

NASA Astrophysics Data System (ADS)

Goto, Tomotsugu; Yagi, Masafumi; Yamauchi, Chisato

2008-12-01

We have performed spatially resolved medium resolution long-slit spectroscopy of a nearby E+A (post-starburst) galaxy system, SDSSJ161330.18+510335.5, with the FOCAS spectrograph mounted on the Subaru Telescope. This E+A galaxy has an obvious companion galaxy 14kpc in front with the velocity difference of 61.8kms-1. Both galaxies have obviously disturbed morphology. Thus, this E+A system provides us with a perfect opportunity to investigate the relation between the post-starburst phenomena and galaxy-galaxy interaction. We have found that the Hδ equivalent width (EW) of the E+A galaxy is greater than 7Å galaxy wide (8.5kpc) with no significant spatial variation. The E+A galaxy has a weak [OIII] emission (EW ~ 1Å) offset by ~2.6kpc from the peak of the Balmer absorption lines. We detected a rotational velocity in the companion galaxy of >175kms-1. The progenitor of the companion may have been a rotationally supported, but yet passive S0 galaxy. We did not detect significant rotation on the E+A galaxy. A metallicity estimate based on the r - H colour suggests Z = 0.008 and 0.02, for the E+A and the companion galaxies, respectively. Assuming these metallicity estimates, the age of the E+A galaxy after quenching the star formation is estimated to be 100-500Myr, with its centre having a slightly younger stellar population. The companion galaxy is estimated to have an older stellar population of >2Gyr of age with no significant spatial variation. These findings are inconsistent with a simple picture where the dynamical interaction creates infall of the gas reservoir that causes the central starburst/post-starburst. Instead, our results present an important example where the galaxy-galaxy interaction can trigger a galaxy-wide post-starburst phenomenon. Based on data collected at the Subaru Telescope, which is operated by the National Astronomical Observatory of Japan. E-mail: tomo@ifa.hawaii.edu ‡ Japan Society for the Promotion of Science (JSPS) SPD Fellow. �

Spatially Resolved Chemical Imaging for Biosignature Analysis: Terrestrial and Extraterrestrial Examples

NASA Astrophysics Data System (ADS)

Bhartia, R.; Wanger, G.; Orphan, V. J.; Fries, M.; Rowe, A. R.; Nealson, K. H.; Abbey, W. J.; DeFlores, L. P.; Beegle, L. W.

2014-12-01

Detection of in situ biosignatures on terrestrial and planetary missions is becoming increasingly more important. Missions that target the Earth's deep biosphere, Mars, moons of Jupiter (including Europa), moons of Saturn (Titan and Enceladus), and small bodies such as asteroids or comets require methods that enable detection of materials for both in-situ analysis that preserve context and as a means to select high priority sample for return to Earth. In situ instrumentation for biosignature detection spans a wide range of analytical and spectroscopic methods that capitalize on amino acid distribution, chirality, lipid composition, isotopic fractionation, or textures that persist in the environment. Many of the existing analytical instruments are bulk analysis methods and while highly sensitive, these require sample acquisition and sample processing. However, by combining with triaging spectroscopic methods, biosignatures can be targeted on a surface and preserve spatial context (including mineralogy, textures, and organic distribution). To provide spatially correlated chemical analysis at multiple spatial scales (meters to microns) we have employed a dual spectroscopic approach that capitalizes on high sensitivity deep UV native fluorescence detection and high specificity deep UV Raman analysis.. Recently selected as a payload on the Mars 2020 mission, SHERLOC incorporates these optical methods for potential biosignatures detection on Mars. We present data from both Earth analogs that operate as our only examples known biosignatures and meteorite samples that provide an example of abiotic organic formation, and demonstrate how provenance effects the spatial distribution and composition of organics.

Resolving the Massive Binary Wind Interaction Of Eta Carinae with HST/STIS

NASA Technical Reports Server (NTRS)

Gull, Theodore; Nielsen, K.; Corcoran, M.; Hillier, J.; Madura, T.; Hamaguchi, K.; Kober, G.; Owocki, S.; Russell, C.; Okazaki, A.;

A direct electron detector for time-resolved MeV electron microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vecchione, T.; Denes, P.; Jobe, R. K.

The introduction of direct electron detectors enabled the structural biology revolution of cryogenic electron microscopy. Direct electron detectors are now expected to have a similarly dramatic impact on time-resolved MeV electron microscopy, particularly by enabling both spatial and temporal jitter correction. Here we report on the commissioning of a direct electron detector for time-resolved MeV electron microscopy. The direct electron detector demonstrated MeV single electron sensitivity and is capable of recording megapixel images at 180 Hz. The detector has a 15-bit dynamic range, better than 30-μmμm spatial resolution and less than 20 analogue-to-digital converter count RMS pixel noise. The uniquemore » capabilities of the direct electron detector and the data analysis required to take advantage of these capabilities are presented. The technical challenges associated with generating and processing large amounts of data are also discussed.« less

Influence of hydrostatic pressure on dynamics and spatial distribution of protein partial molar volume: time-resolved surficial Kirkwood-Buff approach.

PubMed

Yu, Isseki; Tasaki, Tomohiro; Nakada, Kyoko; Nagaoka, Masataka

2010-09-30

The influence of hydrostatic pressure on the partial molar volume (PMV) of the protein apomyoglobin (AMb) was investigated by all-atom molecular dynamics (MD) simulations. Using the time-resolved Kirkwood-Buff (KB) approach, the dynamic behavior of the PMV was identified. The simulated time average value of the PMV and its reduction by 3000 bar pressurization correlated with experimental data. In addition, with the aid of the surficial KB integral method, we obtained the spatial distributions of the components of PMV to elucidate the detailed mechanism of the PMV reduction. New R-dependent PMV profiles identified the regions that increase or decrease the PMV under the high pressure condition. The results indicate that besides the hydration in the vicinity of the protein surface, the outer space of the first hydration layer also significantly influences the total PMV change. These results provide a direct and detailed picture of pressure induced PMV reduction.

Quantitative time-resolved chemoproteomics reveals that stable O-GlcNAc regulates box C/D snoRNP biogenesis

PubMed Central

Qin, Wei; Lv, Pinou; Fan, Xinqi; Quan, Baiyi; Zhu, Yuntao; Qin, Ke; Chen, Ying; Wang, Chu

2017-01-01

O-linked GlcNAcylation (O-GlcNAcylation), a ubiquitous posttranslational modification on intracellular proteins, is dynamically regulated in cells. To analyze the turnover dynamics of O-GlcNAcylated proteins, we developed a quantitative time-resolved O-linked GlcNAc proteomics (qTOP) strategy based on metabolic pulse-chase labeling with an O-GlcNAc chemical reporter and stable isotope labeling with amino acids in cell culture (SILAC). Applying qTOP, we quantified the turnover rates of 533 O-GlcNAcylated proteins in NIH 3T3 cells and discovered that about 14% exhibited minimal removal of O-GlcNAc or degradation of protein backbones. The stability of those hyperstable O-GlcNAcylated proteins was more sensitive to O-GlcNAcylation inhibition compared with the more dynamic populations. Among the hyperstable population were three core proteins of box C/D small nucleolar ribonucleoprotein complexes (snoRNPs): fibrillarin (FBL), nucleolar protein 5A (NOP56), and nucleolar protein 5 (NOP58). We showed that O-GlcNAcylation stabilized these proteins and was essential for snoRNP assembly. Blocking O-GlcNAcylation on FBL altered the 2′-O-methylation of rRNAs and impaired cancer cell proliferation and tumor formation in vivo. PMID:28760965

Spatially resolved spectrophotometry of Comet P/Stephan-Oterma

NASA Technical Reports Server (NTRS)

Cochran, A. L.; Barker, E. S.

1985-01-01

Observations of Comet P/Stephan-Oterma were made with an Intensified Dissector Scanner spectrograph on the McDonald Observatory 2.7-m telescope during the period from July 1980 to February 1981. These spectra cover a range of heliocentric distances from 2.3 AU preperihelion to 1.8 AU postperihelion. A small aperture was used to map the spatial distributions of the gases in the coma. Column densities of the observed cometary emissions (CN, C3, CH, and C2) were calculated, and it is shown that Stephan-Oterma appeared nearly spherically symmetric. These date are used by Cochran (1985) to constrain chemical models of Stephan-Oterma.

Daylight time-resolved photographs of lightning.

PubMed

Qrville, R E; Lala, G G; Idone, V P

1978-07-07

Lightning dart leaders and return strokes have been recorded in daylight with both good spatial resolution and good time resolution as part of the Thunder-storm Research International Program. The resulting time-resolved photographs are apparently equivalent to the best data obtained earlier only at night. Average two-dimensional return stroke velocities in four subsequent strokes between the ground and a height of 1400 meters were approximately 1.3 x 10(8) meters per second. The estimated systematic error is 10 to 15 percent.

Quantitative Analysis of Hepatitis C NS5A Viral Protein Dynamics on the ER Surface.

PubMed

Knodel, Markus M; Nägel, Arne; Reiter, Sebastian; Vogel, Andreas; Targett-Adams, Paul; McLauchlan, John; Herrmann, Eva; Wittum, Gabriel

2018-01-08

Exploring biophysical properties of virus-encoded components and their requirement for virus replication is an exciting new area of interdisciplinary virological research. To date, spatial resolution has only rarely been analyzed in computational/biophysical descriptions of virus replication dynamics. However, it is widely acknowledged that intracellular spatial dependence is a crucial component of virus life cycles. The hepatitis C virus-encoded NS5A protein is an endoplasmatic reticulum (ER)-anchored viral protein and an essential component of the virus replication machinery. Therefore, we simulate NS5A dynamics on realistic reconstructed, curved ER surfaces by means of surface partial differential equations (sPDE) upon unstructured grids. We match the in silico NS5A diffusion constant such that the NS5A sPDE simulation data reproduce experimental NS5A fluorescence recovery after photobleaching (FRAP) time series data. This parameter estimation yields the NS5A diffusion constant. Such parameters are needed for spatial models of HCV dynamics, which we are developing in parallel but remain qualitative at this stage. Thus, our present study likely provides the first quantitative biophysical description of the movement of a viral component. Our spatio-temporal resolved ansatz paves new ways for understanding intricate spatial-defined processes central to specfic aspects of virus life cycles.

Quantitative Analysis of Hepatitis C NS5A Viral Protein Dynamics on the ER Surface

PubMed Central

Nägel, Arne; Reiter, Sebastian; Vogel, Andreas; McLauchlan, John; Herrmann, Eva; Wittum, Gabriel

2018-01-01

Exploring biophysical properties of virus-encoded components and their requirement for virus replication is an exciting new area of interdisciplinary virological research. To date, spatial resolution has only rarely been analyzed in computational/biophysical descriptions of virus replication dynamics. However, it is widely acknowledged that intracellular spatial dependence is a crucial component of virus life cycles. The hepatitis C virus-encoded NS5A protein is an endoplasmatic reticulum (ER)-anchored viral protein and an essential component of the virus replication machinery. Therefore, we simulate NS5A dynamics on realistic reconstructed, curved ER surfaces by means of surface partial differential equations (sPDE) upon unstructured grids. We match the in silico NS5A diffusion constant such that the NS5A sPDE simulation data reproduce experimental NS5A fluorescence recovery after photobleaching (FRAP) time series data. This parameter estimation yields the NS5A diffusion constant. Such parameters are needed for spatial models of HCV dynamics, which we are developing in parallel but remain qualitative at this stage. Thus, our present study likely provides the first quantitative biophysical description of the movement of a viral component. Our spatio-temporal resolved ansatz paves new ways for understanding intricate spatial-defined processes central to specfic aspects of virus life cycles. PMID:29316722

Optical computed tomography for spatially isotropic four-dimensional imaging of live single cells

PubMed Central

Kelbauskas, Laimonas; Shetty, Rishabh; Cao, Bin; Wang, Kuo-Chen; Smith, Dean; Wang, Hong; Chao, Shi-Hui; Gangaraju, Sandhya; Ashcroft, Brian; Kritzer, Margaret; Glenn, Honor; Johnson, Roger H.; Meldrum, Deirdre R.

2017-01-01

Quantitative three-dimensional (3D) computed tomography (CT) imaging of living single cells enables orientation-independent morphometric analysis of the intricacies of cellular physiology. Since its invention, x-ray CT has become indispensable in the clinic for diagnostic and prognostic purposes due to its quantitative absorption-based imaging in true 3D that allows objects of interest to be viewed and measured from any orientation. However, x-ray CT has not been useful at the level of single cells because there is insufficient contrast to form an image. Recently, optical CT has been developed successfully for fixed cells, but this technology called Cell-CT is incompatible with live-cell imaging due to the use of stains, such as hematoxylin, that are not compatible with cell viability. We present a novel development of optical CT for quantitative, multispectral functional 4D (three spatial + one spectral dimension) imaging of living single cells. The method applied to immune system cells offers truly isotropic 3D spatial resolution and enables time-resolved imaging studies of cells suspended in aqueous medium. Using live-cell optical CT, we found a heterogeneous response to mitochondrial fission inhibition in mouse macrophages and differential basal remodeling of small (0.1 to 1 fl) and large (1 to 20 fl) nuclear and mitochondrial structures on a 20- to 30-s time scale in human myelogenous leukemia cells. Because of its robust 3D measurement capabilities, live-cell optical CT represents a powerful new tool in the biomedical research field. PMID:29226240

SDSS-IV MaNGA - the spatially resolved transition from star formation to quiescence

NASA Astrophysics Data System (ADS)

Belfiore, Francesco; Maiolino, Roberto; Maraston, Claudia; Emsellem, Eric; Bershady, Matthew A.; Masters, Karen L.; Bizyaev, Dmitry; Boquien, Médéric; Brownstein, Joel R.; Bundy, Kevin; Diamond-Stanic, Aleksandar M.; Drory, Niv; Heckman, Timothy M.; Law, David R.; Malanushenko, Olena; Oravetz, Audrey; Pan, Kaike; Roman-Lopes, Alexandre; Thomas, Daniel; Weijmans, Anne-Marie; Westfall, Kyle B.; Yan, Renbin

2017-04-01

Using spatially resolved spectroscopy from SDSS-IV MaNGA we have demonstrated that low ionization emission-line regions (LIERs) in local galaxies result from photoionization by hot evolved stars, not active galactic nuclei, hence tracing galactic region hosting old stellar population where, despite the presence of ionized gas, star formation is no longer occurring. LIERs are ubiquitous in both quiescent galaxies and in the central regions of galaxies where star formation takes place at larger radii. We refer to these two classes of galaxies as extended LIER (eLIER) and central LIER (cLIER) galaxies, respectively. cLIERs are late-type galaxies primarily spread across the green valley, in the transition region between the star formation main sequence and quiescent galaxies. These galaxies display regular disc rotation in both stars and gas, although featuring a higher central stellar velocity dispersion than star-forming galaxies of the same mass. cLIERs are consistent with being slowly quenched inside-out; the transformation is associated with massive bulges, pointing towards the importance of bulge growth via secular evolution. eLIERs are morphologically early types and are indistinguishable from passive galaxies devoid of line emission in terms of their stellar populations, morphology and central stellar velocity dispersion. Ionized gas in eLIERs shows both disturbed and disc-like kinematics. When a large-scale flow/rotation is observed in the gas, it is often misaligned relative to the stellar component. These features indicate that eLIERs are passive galaxies harbouring a residual cold gas component, acquired mostly via external accretion. Importantly, quiescent galaxies devoid of line emission reside in denser environments and have significantly higher satellite fraction than eLIERs. Environmental effects thus represent the likely cause for the existence of line-less galaxies on the red sequence.

In situ probing of doping- and stress-mediated phase transitions in a single-crystalline VO2 nanobeam by spatially resolved Raman spectroscopy

NASA Astrophysics Data System (ADS)

Chang, Sung-Jin; Park, Jong Bae; Lee, Gaehang; Kim, Hae Jin; Lee, Jin-Bae; Bae, Tae-Sung; Han, Young-Kyu; Park, Tae Jung; Huh, Yun Suk; Hong, Woong-Ki

2014-06-01

We demonstrate an experimental in situ observation of the temperature-dependent evolution of doping- and stress-mediated structural phase transitions in an individual single-crystalline VO2 nanobeam on a Au-coated substrate under exposure to hydrogen gas using spatially resolved Raman spectroscopy. The nucleation temperature of the rutile R structural phase in the VO2 nanobeam upon heating under hydrogen gas was lower than that under air. The spatial structural phase evolution behavior along the length of the VO2 nanobeam under hydrogen gas upon heating was much more inhomogeneous than that along the length of the same nanobeam under air. The triclinic T phase of the VO2 nanobeam upon heating under hydrogen gas transformed to the R phase and this R phase was stabilized even at room temperature in air after sample cooling. In particular, after the VO2 nanobeam with the R phase was annealed at approximately 250 °C in air, it exhibited the monoclinic M1 phase (not the T phase) at room temperature during heating and cooling cycles. These results were attributed to the interplay between hydrogen doping and stress associated with nanobeam-substrate interactions. Our study has important implications for engineering metal-insulator transition properties and developing functional devices based on VO2 nanostructures through doping and stress.We demonstrate an experimental in situ observation of the temperature-dependent evolution of doping- and stress-mediated structural phase transitions in an individual single-crystalline VO2 nanobeam on a Au-coated substrate under exposure to hydrogen gas using spatially resolved Raman spectroscopy. The nucleation temperature of the rutile R structural phase in the VO2 nanobeam upon heating under hydrogen gas was lower than that under air. The spatial structural phase evolution behavior along the length of the VO2 nanobeam under hydrogen gas upon heating was much more inhomogeneous than that along the length of the same nanobeam under

Mesoscopic structural phase progression in photo-excited VO 2 revealed by time-resolved x-ray diffraction microscopy

DOE PAGES

Zhu, Yi; Cai, Zhonghou; Chen, Pice; ...

2016-02-26

Dynamical phase separation during a solid-solid phase transition poses a challenge for understanding the fundamental processes in correlated materials. Critical information underlying a phase transition, such as localized phase competition, is difficult to reveal by measurements that are spatially averaged over many phase seperated regions. The ability to simultanousely track the spatial and temporal evolution of such systems is essential to understanding mesoscopic processes during a phase transition. Using state-of- the-art time-resolved hard x-ray diffraction microscopy, we directly visualize the structural phase progression in a VO 2 film upon photoexcitation. Following a homogenous in-plane optical excitation, the phase transformation ismore » initiated at discrete sites and completed by the growth of one lattice structure into the other, instead of a simultaneous isotropic lattice symmetry change. The time-dependent x-ray diffraction spatial maps show that the in-plane phase progression in laser-superheated VO 2 is via a displacive lattice transformation as a result of relaxation from an excited monoclinic phase into a rutile phase. The speed of the phase front progression is quantitatively measured, which is faster than the process driven by in-plane thermal diffusion but slower than the sound speed in VO 2. Lastly, the direct visualization of localized structural changes in the time domain opens a new avenue to study mesoscopic processes in driven systems.« less

Mesoscopic structural phase progression in photo-excited VO2 revealed by time-resolved x-ray diffraction microscopy

NASA Astrophysics Data System (ADS)

Zhu, Yi; Cai, Zhonghou; Chen, Pice; Zhang, Qingteng; Highland, Matthew J.; Jung, Il Woong; Walko, Donald A.; Dufresne, Eric M.; Jeong, Jaewoo; Samant, Mahesh G.; Parkin, Stuart S. P.; Freeland, John W.; Evans, Paul G.; Wen, Haidan

2016-02-01

Dynamical phase separation during a solid-solid phase transition poses a challenge for understanding the fundamental processes in correlated materials. Critical information underlying a phase transition, such as localized phase competition, is difficult to reveal by measurements that are spatially averaged over many phase separated regions. The ability to simultaneously track the spatial and temporal evolution of such systems is essential to understanding mesoscopic processes during a phase transition. Using state-of-the-art time-resolved hard x-ray diffraction microscopy, we directly visualize the structural phase progression in a VO2 film upon photoexcitation. Following a homogenous in-plane optical excitation, the phase transformation is initiated at discrete sites and completed by the growth of one lattice structure into the other, instead of a simultaneous isotropic lattice symmetry change. The time-dependent x-ray diffraction spatial maps show that the in-plane phase progression in laser-superheated VO2 is via a displacive lattice transformation as a result of relaxation from an excited monoclinic phase into a rutile phase. The speed of the phase front progression is quantitatively measured, and is faster than the process driven by in-plane thermal diffusion but slower than the sound speed in VO2. The direct visualization of localized structural changes in the time domain opens a new avenue to study mesoscopic processes in driven systems.

Mesoscopic structural phase progression in photo-excited VO2 revealed by time-resolved x-ray diffraction microscopy.

PubMed

Zhu, Yi; Cai, Zhonghou; Chen, Pice; Zhang, Qingteng; Highland, Matthew J; Jung, Il Woong; Walko, Donald A; Dufresne, Eric M; Jeong, Jaewoo; Samant, Mahesh G; Parkin, Stuart S P; Freeland, John W; Evans, Paul G; Wen, Haidan

2016-02-26

Dynamical phase separation during a solid-solid phase transition poses a challenge for understanding the fundamental processes in correlated materials. Critical information underlying a phase transition, such as localized phase competition, is difficult to reveal by measurements that are spatially averaged over many phase separated regions. The ability to simultaneously track the spatial and temporal evolution of such systems is essential to understanding mesoscopic processes during a phase transition. Using state-of-the-art time-resolved hard x-ray diffraction microscopy, we directly visualize the structural phase progression in a VO2 film upon photoexcitation. Following a homogenous in-plane optical excitation, the phase transformation is initiated at discrete sites and completed by the growth of one lattice structure into the other, instead of a simultaneous isotropic lattice symmetry change. The time-dependent x-ray diffraction spatial maps show that the in-plane phase progression in laser-superheated VO2 is via a displacive lattice transformation as a result of relaxation from an excited monoclinic phase into a rutile phase. The speed of the phase front progression is quantitatively measured, and is faster than the process driven by in-plane thermal diffusion but slower than the sound speed in VO2. The direct visualization of localized structural changes in the time domain opens a new avenue to study mesoscopic processes in driven systems.

Measurement of fluorophore concentrations and fluorescence quantum yield in tissue-simulating phantoms using three diffusion models of steady-state spatially resolved fluorescence.

PubMed

Diamond, Kevin R; Farrell, Thomas J; Patterson, Michael S

2003-12-21

Steady-state diffusion theory models of fluorescence in tissue have been investigated for recovering fluorophore concentrations and fluorescence quantum yield. Spatially resolved fluorescence, excitation and emission reflectance Carlo simulations, and measured using a multi-fibre probe on tissue-simulating phantoms containing either aluminium phthalocyanine tetrasulfonate (AlPcS4), Photofrin meso-tetra-(4-sulfonatophenyl)-porphine dihydrochloride The accuracy of the fluorophore concentration and fluorescence quantum yield recovered by three different models of spatially resolved fluorescence were compared. The models were based on: (a) weighted difference of the excitation and emission reflectance, (b) fluorescence due to a point excitation source or (c) fluorescence due to a pencil beam excitation source. When literature values for the fluorescence quantum yield were used for each of the fluorophores, the fluorophore absorption coefficient (and hence concentration) at the excitation wavelength (mu(a,x,f)) was recovered with a root-mean-square accuracy of 11.4% using the point source model of fluorescence and 8.0% using the more complicated pencil beam excitation model. The accuracy was calculated over a broad range of optical properties and fluorophore concentrations. The weighted difference of reflectance model performed poorly, with a root-mean-square error in concentration of about 50%. Monte Carlo simulations suggest that there are some situations where the weighted difference of reflectance is as accurate as the other two models, although this was not confirmed experimentally. Estimates of the fluorescence quantum yield in multiple scattering media were also made by determining mu(a,x,f) independently from the fitted absorption spectrum and applying the various diffusion theory models. The fluorescence quantum yields for AlPcS4 and TPPS4 were calculated to be 0.59 +/- 0.03 and 0.121 +/- 0.001 respectively using the point source model, and 0.63 +/- 0.03 and 0

A spatially resolved fuel-based inventory of Utah and Colorado oil and natural gas emissions

NASA Astrophysics Data System (ADS)

Gorchov Negron, A.; McDonald, B. C.; De Gouw, J. A.; Frost, G. J.

2015-12-01

A fuel-based approach is presented for estimating emissions from US oil and natural gas production that utilizes state-level fuel surveys of oil and gas engine activity, well-level production data, and emission factors for oil and gas equipment. Emissions of carbon dioxide (CO2) and nitrogen oxides (NOx) are mapped on a 4 km x 4 km horizontal grid for 2013-14 in Utah and Colorado. Emission sources include combustion from exploration (e.g., drilling), production (e.g., heaters, dehydrators, and compressor engines), and natural gas processing plants, which comprise a large fraction of the local combustion activity in oil and gas basins. Fuel-based emission factors of NOx are from the U.S. Environmental Protection Agency, and applied to spatially-resolved maps of CO2 emissions. Preliminary NOx emissions from this study are estimated for the Uintah Basin, Utah, to be ~5300 metric tons of NO2-equivalent in 2013. Our result compares well with an observations-based top-down emissions estimate of NOx derived from a previous study, ~4200 metric tons of NO2-equivalent. By contrast, the 2011 National Emissions Inventory estimates oil and gas emissions of NOx to be ~3 times higher than our study in the Uintah Basin. We intend to expand our fuel-based approach to map combustion-related emissions in other U.S. oil and natural gas basins and compare with additional observational datasets.

Spatially resolved SO2 flux emissions from Mt Etna

PubMed Central

Bitetto, M.; Delle Donne, D.; Tamburello, G.; Battaglia, A.; Coltelli, M.; Patanè, D.; Prestifilippo, M.; Sciotto, M.; Aiuppa, A.

2016-01-01

Abstract We report on a systematic record of SO2 flux emissions from individual vents of Etna volcano (Sicily), which we obtained using a permanent UV camera network. Observations were carried out in summer 2014, a period encompassing two eruptive episodes of the New South East Crater (NSEC) and a fissure‐fed eruption in the upper Valle del Bove. We demonstrate that our vent‐resolved SO2 flux time series allow capturing shifts in activity from one vent to another and contribute to our understanding of Etna's shallow plumbing system structure. We find that the fissure eruption contributed ~50,000 t of SO2 or ~30% of the SO2 emitted by the volcano during the 5 July to 10 August eruptive interval. Activity from this eruptive vent gradually vanished on 10 August, marking a switch of degassing toward the NSEC. Onset of degassing at the NSEC was a precursory to explosive paroxysmal activity on 11–15 August. PMID:27773952

Spatially resolved SO2 flux emissions from Mt Etna.

PubMed

D'Aleo, R; Bitetto, M; Delle Donne, D; Tamburello, G; Battaglia, A; Coltelli, M; Patanè, D; Prestifilippo, M; Sciotto, M; Aiuppa, A

2016-07-28

We report on a systematic record of SO 2 flux emissions from individual vents of Etna volcano (Sicily), which we obtained using a permanent UV camera network. Observations were carried out in summer 2014, a period encompassing two eruptive episodes of the New South East Crater (NSEC) and a fissure-fed eruption in the upper Valle del Bove. We demonstrate that our vent-resolved SO 2 flux time series allow capturing shifts in activity from one vent to another and contribute to our understanding of Etna's shallow plumbing system structure. We find that the fissure eruption contributed ~50,000 t of SO 2 or ~30% of the SO 2 emitted by the volcano during the 5 July to 10 August eruptive interval. Activity from this eruptive vent gradually vanished on 10 August, marking a switch of degassing toward the NSEC. Onset of degassing at the NSEC was a precursory to explosive paroxysmal activity on 11-15 August.

Quantitative Imaging of Gut Microbiota Spatial Organization

PubMed Central

Earle, Kristen A.; Billings, Gabriel; Sigal, Michael; Lichtman, Joshua S.; Hansson, Gunnar C.; Elias, Joshua E.; Amieva, Manuel R.; Huang, Kerwyn Casey; Sonnenburg, Justin L.

2015-01-01

Summary Genomic technologies have significantly advanced our understanding of the composition and diversity of host-associated microbial populations. However, their spatial organization and functional interactions relative to the host have been more challenging to study. Here we present a pipeline for the assessment of intestinal microbiota localization within immunofluorescence images of fixed gut cross-sections that includes a flexible software package, BacSpace, for high-throughput quantification of microbial organization. Applying this pipeline to gnotobiotic and human microbiota-colonized mice, we demonstrate that elimination of microbiota accessible carbohydrates (MACs) from the diet results in thinner mucus in the distal colon, increased proximity of microbes to the epithelium, and heightened expression of the inflammatory marker REG3β. Measurements of microbe-microbe proximity reveal that a MAC-deficient diet alters monophyletic spatial clustering. Furthermore, we quantify the invasion of Helicobacter pylori into the glands of the mouse stomach relative to host mitotic progenitor cells, illustrating the generalizability of this approach. PMID:26439864

Spatially resolved organic analysis of the Allende meteorite

NASA Technical Reports Server (NTRS)

Zenobi, Renato; Philippoz, Jean-Michel; Zare, Richard N.; Buseck, Peter R.

1989-01-01

The distribution of polycyclic aromatic hydrocarbons (PAHs) in the Allende meteorite has been probed with two-step laser desorption/laser multiphoton ionization mass spectrometry. This method allows direct in situ analysis with a spatial resolution of 1 sq mm or better of selected organic molecules. Spectra from freshly fractured interior surfaces of the meteorite show that PAH concentrations are locally high compared to the average concentrations found by wet chemical analysis of pulverized samples. The data suggest that the PAHs are primarily associated with the fine-grained matrix, where the organic polymer occurs. In addition, highly substituted PAH skeletons were observed. Interiors of individual chondrules were devoid of PAHs at the detection limit (about 0.05 ppm).

A direct electron detector for time-resolved MeV electron microscopy

DOE PAGES

Vecchione, T.; Denes, P.; Jobe, R. K.; ...

2017-03-15

The introduction of direct electron detectors enabled the structural biology revolution of cryogenic electron microscopy. Direct electron detectors are now expected to have a similarly dramatic impact on time-resolved MeV electron microscopy, particularly by enabling both spatial and temporal jitter correction. Here in this paper, we report on the commissioning of a direct electron detector for time-resolved MeV electron microscopy. The direct electron detector demonstrated MeV single electron sensitivity and is capable of recording megapixel images at 180 Hz. The detector has a 15-bit dynamic range, better than 30-μm spatial resolution and less than 20 analogue-to-digital converter count RMS pixelmore » noise. The unique capabilities of the direct electron detector and the data analysis required to take advantage of these capabilities are presented. The technical challenges associated with generating and processing large amounts of data are also discussed.« less

A direct electron detector for time-resolved MeV electron microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vecchione, T.; Denes, P.; Jobe, R. K.

The introduction of direct electron detectors enabled the structural biology revolution of cryogenic electron microscopy. Direct electron detectors are now expected to have a similarly dramatic impact on time-resolved MeV electron microscopy, particularly by enabling both spatial and temporal jitter correction. Here in this paper, we report on the commissioning of a direct electron detector for time-resolved MeV electron microscopy. The direct electron detector demonstrated MeV single electron sensitivity and is capable of recording megapixel images at 180 Hz. The detector has a 15-bit dynamic range, better than 30-μm spatial resolution and less than 20 analogue-to-digital converter count RMS pixelmore » noise. The unique capabilities of the direct electron detector and the data analysis required to take advantage of these capabilities are presented. The technical challenges associated with generating and processing large amounts of data are also discussed.« less

Exotic singularities and spatially curved loop quantum cosmology

DOE Office of Scientific and Technical Information (OSTI.GOV)

Singh, Parampreet; Perimeter Institute for Theoretical Physics, 31 Caroline Street North, Waterloo, Ontario N2L 2Y5; Vidotto, Francesca

2011-03-15

We investigate the occurrence of various exotic spacelike singularities in the past and the future evolution of k={+-}1 Friedmann-Robertson-Walker model and loop quantum cosmology using a sufficiently general phenomenological model for the equation of state. We highlight the nontrivial role played by the intrinsic curvature for these singularities and the new physics which emerges at the Planck scale. We show that quantum gravity effects generically resolve all strong curvature singularities including big rip and big freeze singularities. The weak singularities, which include sudden and big brake singularities, are ignored by quantum gravity when spatial curvature is negative, as was previouslymore » found for the spatially flat model. Interestingly, for the spatially closed model there exist cases where weak singularities may be resolved when they occur in the past evolution. The spatially closed model exhibits another novel feature. For a particular class of equation of state, this model also exhibits an additional physical branch in loop quantum cosmology, a baby universe separated from the parent branch. Our analysis generalizes previous results obtained on the resolution of strong curvature singularities in flat models to isotropic spacetimes with nonzero spatial curvature.« less

Studies of electronic structure of ZnO grain boundary and its proximity by using spatially resolved electron energy loss spectroscopy

NASA Astrophysics Data System (ADS)

Ong, H. C.; Dai, J. Y.; Du, G. T.

2002-07-01

The low electron energy loss and complex dielectric functions of an arbitrary grain boundary and its proximity in ZnO thin films have been studied by using the spatially resolved electron energy loss spectroscopy. The critical point parameters have been determined by fitting the dielectric functions simultaneously with analytical line shape model. Gradual changes have been observed in the dielectric functions spectra. The critical points are found to redshift and then blueshift when the electron beam scanned across the grain boundary, which suggest the distinctive electronic structure not only of the grain boundary but also of the depletion region. In addition, comparison has been made between the experiment and the recent theoretical studies to account for the interband transitions that occur in the grain boundaries. Several features predicted by the theory are qualitatively found to be consistent with our results. The presence of dangling bonds instead of bond distortion is attributed to be the major cause of defects in the grain boundaries of ZnO.

Tunable All Reflective Spatial Heterodyne Spectroscopy, A Technique For High Resolving Power Observation OI Defused Emission Line Sources

NASA Astrophysics Data System (ADS)

Hosseini, Seyedeh Sona

The solar system presents a challenge to spectroscopic observers, because it is an astrophysically low energy environment populated with often angularly extended targets (e.g, interplanetary medium, comets, planetary upper atmospheres, and planet and satellite near space environments). Spectroscopy is a proven tool for determining compositional and other properties of remote objects. Narrow band imaging and low resolving spectroscopic measurements provide information about composition, photochemical evolution, energy distribution and density. The extension to high resolving power provides further access to temperature, velocity, isotopic ratios, separation of blended sources, and opacity effects. The drawback of high-resolution spectroscopy comes from the instrumental limitations of lower throughput, the necessity of small entrance apertures, sensitivity, field of view, and large physical instrumental size. These limitations quickly become definitive for faint and/or extended targets and for spacecraft encounters. An emerging technique with promise for the study of faint, extended sources at high resolving power is the all-reflective form of the Spatial Heterodyne Spectrometer (SHS). SHS instruments are compact and naturally possess both high etendue and high resolving power. To achieve similar spectral grasp, grating spectrometers require big telescopes. SHS is a common-path beam Fourier transform interferometer that produces Fizeau fringe pattern for all other wavelengths except the tuned wavelength. Compared to similar Fourier transform Spectrometers (FTS), SHS has considerably relaxed optical tolerances that make it easier to use in the visible and UV spectral ranges. The large etendue of SHS instruments makes them ideal for observations of extended, low surface brightness, isolated emission line sources, while their intrinsically high spectral resolution enables the study of the dynamical and spectral characteristics described above. SHS also combines very

Interplay of point defects, extended defects, and carrier localization in the efficiency droop of InGaN quantum wells light-emitting diodes investigated using spatially resolved electroluminescence and photoluminescence

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lin, Yue, E-mail: yuelin@fjirsm.ac.cn; Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002; Department of Electronic Science and Fujian Engineering Research Center for Solid-State Lighting, Xiamen University, Xiamen, Fujian 361005

2014-01-14

We perform both spatially resolved electroluminescence (SREL) as a function of injection current and spatially resolved photoluminescence (SRPL) as a function of excitation power on InGaN quantum well blue light-emitting diodes to investigate the underlying physics for the phenomenon of the external quantum efficiency (EQE) droop. SREL allows us to study two most commonly observed but distinctly different droop behaviors on a single device, minimizing the ambiguity trying to compare independently fabricated devices. Two representative devices are studied: one with macroscopic scale material non-uniformity, the other being macroscopically uniform, but both with microscopic scale fluctuations. We suggest that the EQE–currentmore » curve reflects the interplay of three effects: nonradiative recombination through point defects, carrier localization due to either In composition or well width fluctuation, and nonradiative recombination of the extended defects, which is common to various optoelectronic devices. By comparing SREL and SRPL, two very different excitation/detection modes, we show that individual singular sites exhibiting either particularly strong or weak emission in SRPL do not usually play any significant and direct role in the EQE droop. We introduce a two-level model that can capture the basic physical processes that dictate the EQE–current dependence and describe the whole operating range of the device from 0.01 to 100 A/cm{sup 2}.« less

Spatially Resolved Genomic, Stable Isotopic, and Lipid Analyses of a Modern Freshwater Microbialite from Cuatro Ciénegas, Mexico

PubMed Central

Nitti, Anthony; Daniels, Camille A.; Siefert, Janet; Souza, Valeria; Hollander, David

2012-01-01

Abstract Microbialites are biologically mediated carbonate deposits found in diverse environments worldwide. To explore the organisms and processes involved in microbialite formation, this study integrated genomic, lipid, and both organic and inorganic stable isotopic analyses to examine five discrete depth horizons spanning the surface 25 mm of a modern freshwater microbialite from Cuatro Ciénegas, Mexico. Distinct bacterial communities and geochemical signatures were observed in each microbialite layer. Photoautotrophic organisms accounted for approximately 65% of the sequences in the surface community and produced biomass with distinctive lipid biomarker and isotopic (δ13C) signatures. This photoautotrophic biomass was efficiently degraded in the deeper layers by heterotrophic organisms, primarily sulfate-reducing proteobacteria. Two spatially distinct zones of carbonate precipitation were observed within the microbialite, with the first zone corresponding to the phototroph-dominated portion of the microbialite and the second zone associated with the presence of sulfate-reducing heterotrophs. The coupling of photoautotrophic production, heterotrophic decomposition, and remineralization of organic matter led to the incorporation of a characteristic biogenic signature into the inorganic CaCO3 matrix. Overall, spatially resolved multidisciplinary analyses of the microbialite enabled correlations to be made between the distribution of specific organisms, precipitation of carbonate, and preservation of unique lipid and isotopic geochemical signatures. These findings are critical for understanding the formation of modern microbialites and have implications for the interpretation of ancient microbialite records. Key Words: Microbial ecology—Microbe-mineral interactions—Microbial mats—Stromatolites—Genomics. Astrobiology 12, 685–698. PMID:22882001

Multiple spatially resolved reflection spectroscopy for in vivo determination of carotenoids in human skin and blood

NASA Astrophysics Data System (ADS)

Darvin, Maxim E.; Magnussen, Björn; Lademann, Juergen; Köcher, Wolfgang

2016-09-01

Non-invasive measurement of carotenoid antioxidants in human skin is one of the important tasks to investigate the skin physiology in vivo. Resonance Raman spectroscopy and reflection spectroscopy are the most frequently used non-invasive techniques in dermatology and skin physiology. In the present study, an improved method based on multiple spatially resolved reflection spectroscopy (MSRRS) was introduced. The results obtained were compared with those obtained using the ‘gold standard’ resonance Raman spectroscopy method and showed strong correlations for the total carotenoid concentration (R  =  0.83) as well as for lycopene (R  =  0.80). The measurement stability was confirmed to be better than 10% within the total temperature range from 5 °C to  +  30 °C and pressure contact between the skin and the MSRRS sensor from 800 Pa to 18 000 Pa. In addition, blood samples taken from the subjects were analyzed for carotenoid concentrations. The MSRRS sensor was calibrated on the blood carotenoid concentrations resulting in being able to predict with a correlation of R  =  0.79. On the basis of blood carotenoids it could be demonstrated that the MSRRS cutaneous measurements are not influenced by Fitzpatrick skin types I-VI. The MSRRS sensor is commercially available under the brand name biozoom.

Spatially resolved, in-situ monitoring of crack growth via the coupling current in aluminum alloy 5083

NASA Astrophysics Data System (ADS)

Williams, Krystaufeux D.

The work discussed in this dissertation is an experimental validation of a body of research that was created to model stress corrosion cracking phenomenon for 304 stainless steels in boiling water reactors. This coupled environment fracture model (CEFM) incorporates the natural laws of the conservation of charge and the differential aeration hypothesis to predict the amount of stress corrosion crack growth as a function of many external environmental variables, including potential, stress intensity, solution conductivity, oxidizer concentrations, and various other environmental parameters. Out of this approach came the concept of the coupling current; a local corrosion current that flows from within cracks, crevices, pits, etc... of a metal or alloy to the external surface. Because of the deterministic approach taken in the mentioned research, the coupling current analysis and CEFM model can be applied to the specific problem of SCC in aluminum alloy 5083 (the alloy of interest for this dissertation that is highly sought after today because of its corrosion resistance and high strength to weight ratio). This dissertation research is specifically devoted to the experimental verification of the coupling current, which results from a coupling between the crack's internal and external environments, by spatially resolving them using the scanning vibrating probe (SVP) as a tool. Hence, through the use of a unique fracture mechanics setup, simultaneous mechanical and local electrochemical data may be obtained, in situ..

Resolved Star Formation in Galaxies Using Slitless Spectroscopy

NASA Astrophysics Data System (ADS)

Pirzkal, Norbert; Finkelstein, Steven L.; Larson, Rebecca L.; Malhotra, Sangeeta; Rhoads, James E.; Ryan, Russell E.; Tilvi, Vithal; FIGS Team

2018-06-01

The ability to spatially resolve individual star-formation regions in distant galaxies and simultaneously extract their physical properties via emission lines is a critical step forward in studying the evolution of galaxies. While efficient, deep slitless spectroscopic observations offer a blurry view of the summed properties of galaxies. We present our studies of resolved star formation over a wide range of redshifts, including high redshift Ly-a sources. The unique capabilities of the WFC3 IR Grism and our two-dimensional emission line method (EM2D) allows us to accurately identify the specific spatial origin of emission lines in galaxies, thus creating a spatial map of star-formation sites in any given galaxy. This method requires the use of multiple position angles on the sky to accurately derive both the location and the observed wavelengths of these emission lines. This has the added benefit of producing better defined redshifts for these sources. Building on our success in applying the EM2D method towards galaxies with [OII]. [OIII], and Ha emission lines, we have also applied EM2D to high redshift (z>6) Ly-a emitting galaxies. We are also able to produce accurate 2D emission line maps (MAP2D) of the Ly-a emission in WFC3 IR grism observations, looking for evidence that a significant amount of resonant scattering is taking place in high redshift galaxies such as in a newly identified z=7.5 Faint Infrared Galaxy Survey (FIGS) Ly-a galaxy.

Tissue oxygenation and haemodynamics measurement with spatially resolved NIRS

NASA Astrophysics Data System (ADS)

Zhang, Y.; Scopesi, F.; Serra, G.; Sun, J. W.; Rolfe, P.

2010-08-01

We describe the use of Near Infrared Spectroscopy (NIRS) for the non-invasive investigation of changes in haemodynamics and oxygenation of human peripheral tissues. The goal was to measure spatial variations of tissue NIRS oxygenation variables, namely deoxy-haemoglobin (HHb), oxy-haemoglobin (HbO2), total haemoglobin (HbT), and thereby to evaluate the responses of the peripheral circulation to imposed physiological challenges. We present a skinfat- muscle heterogeneous tissue model with varying fat thickness up to 15mm and a Monte Carlo simulation of photon transport within this model. The mean partial path length and the mean photon visit depth in the muscle layer were derived for different source-detector spacing. We constructed NIRS instrumentation comprising of light-emitting diodes (LED) as light sources at four wavelengths, 735nm, 760nm, 810nm and 850nm and sensitive photodiodes (PD) as the detectors. Source-detector spacing was varied to perform measurements at different depths within forearm tissue. Changes in chromophore concentration in response to venous and arterial occlusion were calculated using the modified Lambert-Beer Law. Studies in fat and thin volunteers indicated greater sensitivity in the thinner subjects for the tissue oxygenation measurement in the muscle layer. These results were consistent with those found using Monte Carlo simulation. Overall, the results of this investigation demonstrate the usefulness of the NIRS instrument for deriving spatial information from biological tissues.

Noncontact quantitative biomechanical characterization of cardiac muscle using shear wave imaging optical coherence tomography

PubMed Central

Wang, Shang; Lopez, Andrew L.; Morikawa, Yuka; Tao, Ge; Li, Jiasong; Larina, Irina V.; Martin, James F.; Larin, Kirill V.

2014-01-01

We report on a quantitative optical elastographic method based on shear wave imaging optical coherence tomography (SWI-OCT) for biomechanical characterization of cardiac muscle through noncontact elasticity measurement. The SWI-OCT system employs a focused air-puff device for localized loading of the cardiac muscle and utilizes phase-sensitive OCT to monitor the induced tissue deformation. Phase information from the optical interferometry is used to reconstruct 2-D depth-resolved shear wave propagation inside the muscle tissue. Cross-correlation of the displacement profiles at various spatial locations in the propagation direction is applied to measure the group velocity of the shear waves, based on which the Young’s modulus of tissue is quantified. The quantitative feature and measurement accuracy of this method is demonstrated from the experiments on tissue-mimicking phantoms with the verification using uniaxial compression test. The experiments are performed on ex vivo cardiac muscle tissue from mice with normal and genetically altered myocardium. Our results indicate this optical elastographic technique is useful as a noncontact tool to assist the cardiac muscle studies. PMID:25071943

Spectral mechanisms of spatially induced blackness: data and quantitative model.

PubMed

Shinomori, K; Schefrin, B E; Werner, J S

1997-02-01

Spectral efficiency functions and tests of additivity were obtained with three observers to identify possible chromatic contributions to spatially induced blackness. Stimuli consisted of a series of monochromatic (400-700 nm; 10-nm steps), 52-arcmin circular test lights surrounded by broadband (x = 0.31, y = 0.37), 63-138-arcmin annuli of fixed retinal illuminance. The stimuli were imaged on the fovea in Maxwellian view as 500-ms flashes with 10-s interstimulus intervals. Observers decreased the intensity of the test center until it was first perceived as completely black. Action spectra determined for two surround levels [2.5 and 3.5 log trolands] had three sensitivity peaks (at approximately 440, 540, and 600 nm), However, when monochromatic surrounds were adjusted to induce blackness in a broadband center, action spectra were unimodal and identical to functions obtained by heterochromatic flicker photometry. Tests of additivity revealed that when blackness is induced by broadband surround into a bichromatic center, there is an additivity failure of the cancellation type. This additivity failure indicates that blackness induction is influenced, in part, by signals from opponent-chromatic pathways. A quantitative model is presented to account for these data. This model assumes that blackness induction is determined by the ratio of responses to the stimulus center and the annulus, and while signals form the annulus are based only on achromatic information, responses from the center are based on both chromatic and achromatic properties of the stimulus.

Combining qualitative and quantitative spatial and temporal information in a hierarchical structure: Approximate reasoning for plan execution monitoring

NASA Technical Reports Server (NTRS)

Hoebel, Louis J.

1993-01-01

The problem of plan generation (PG) and the problem of plan execution monitoring (PEM), including updating, queries, and resource-bounded replanning, have different reasoning and representation requirements. PEM requires the integration of qualitative and quantitative information. PEM is the receiving of data about the world in which a plan or agent is executing. The problem is to quickly determine the relevance of the data, the consistency of the data with respect to the expected effects, and if execution should continue. Only spatial and temporal aspects of the plan are addressed for relevance in this work. Current temporal reasoning systems are deficient in computational aspects or expressiveness. This work presents a hybrid qualitative and quantitative system that is fully expressive in its assertion language while offering certain computational efficiencies. In order to proceed, methods incorporating approximate reasoning using hierarchies, notions of locality, constraint expansion, and absolute parameters need be used and are shown to be useful for the anytime nature of PEM.

Time-resolved measurement of global synchronization in the dust acoustic wave

NASA Astrophysics Data System (ADS)

Williams, J. D.

2014-10-01

A spatially and temporally resolved measurement of the synchronization of the naturally occurring dust acoustic wave to an external drive and the relaxation from the driven wave mode back to the naturally occuring wave mode is presented. This measurement provides a time-resolved measurement of the synchronization of the self-excited dust acoustic wave with an external drive and the return to the self-excited mode. It is observed that the wave synchronizes to the external drive in a distinct time-dependent fashion, while there is an immediate loss of synchronization when the external modulation is discontinued.

Operando observations of solid-state electrochemical reactions in Li-ion batteries by spatially resolved TEM EELS and electron holography.

PubMed

Yamamoto, Kazuo; Iriyama, Yasutoshi; Hirayama, Tsukasa

2017-02-08

All-solid-state Li-ion batteries having incombustible solid electrolytes are promising energy storage devices because they have significant advantages in terms of safety, lifetime and energy density. Electrochemical reactions, namely, Li-ion insertion/extraction reactions, commonly occur around the nanometer-scale interfaces between the electrodes and solid electrolytes. Thus, transmission electron microscopy (TEM) is an appropriate technique to directly observe such reactions, providing important information for understanding the fundamental solid-state electrochemistry and improving battery performance. In this review, we introduce two types of TEM techniques for operando observations of battery reactions, spatially resolved electron energy-loss spectroscopy in a TEM mode for direct detection of the Li concentration profiles and electron holography for observing the electric potential changes due to Li-ion insertion/extraction reactions. We visually show how Li-ion insertion/extractions affect the crystal structures, electronic structures, and local electric potential during the charge-discharge processes in these batteries. © The Author 2016. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Conflict resolved: On the role of spatial attention in reading and color naming tasks.

PubMed

Robidoux, Serje; Besner, Derek

2015-12-01

The debate about whether or not visual word recognition requires spatial attention has been marked by a conflict: the results from different tasks yield different conclusions. Experiments in which the primary task is reading based show no evidence that unattended words are processed, whereas when the primary task is color identification, supposedly unattended words do affect processing. However, the color stimuli used to date does not appear to demand as much spatial attention as explicit word reading tasks. We first identify a color stimulus that requires as much spatial attention to identify as does a word. We then demonstrate that when spatial attention is appropriately captured, distractor words in unattended locations do not affect color identification. We conclude that there is no word identification without spatial attention.

Accounting for spatial effects in land use regression for urban air pollution modeling.

PubMed

Bertazzon, Stefania; Johnson, Markey; Eccles, Kristin; Kaplan, Gilaad G

2015-01-01

In order to accurately assess air pollution risks, health studies require spatially resolved pollution concentrations. Land-use regression (LUR) models estimate ambient concentrations at a fine spatial scale. However, spatial effects such as spatial non-stationarity and spatial autocorrelation can reduce the accuracy of LUR estimates by increasing regression errors and uncertainty; and statistical methods for resolving these effects--e.g., spatially autoregressive (SAR) and geographically weighted regression (GWR) models--may be difficult to apply simultaneously. We used an alternate approach to address spatial non-stationarity and spatial autocorrelation in LUR models for nitrogen dioxide. Traditional models were re-specified to include a variable capturing wind speed and direction, and re-fit as GWR models. Mean R(2) values for the resulting GWR-wind models (summer: 0.86, winter: 0.73) showed a 10-20% improvement over traditional LUR models. GWR-wind models effectively addressed both spatial effects and produced meaningful predictive models. These results suggest a useful method for improving spatially explicit models. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

Composite x-ray pinholes for time-resolved microphotography of laser compressed targets.

PubMed

Attwood, D T; Weinstein, B W; Wuerker, R F

1977-05-01

Composite x-ray pinholes having dichroic properties are presented. These pinholes permit both x-ray imaging and visible alignment with micron accuracy by presenting different apparent apertures in these widely disparate regions of the spectrum. Their use is mandatory in certain applications in which the x-ray detection consists of a limited number of resolvable elements whose use one wishes to maximize. Mating the pinhole camera with an x-ray streaking camera is described, along with experiments which spatially and temporally resolve the implosion of laser irradiated targets.

Resolvent-based modeling of passive scalar dynamics in wall-bounded turbulence

NASA Astrophysics Data System (ADS)

Dawson, Scott; Saxton-Fox, Theresa; McKeon, Beverley

2017-11-01

The resolvent formulation of the Navier-Stokes equations expresses the system state as the output of a linear (resolvent) operator acting upon a nonlinear forcing. Previous studies have demonstrated that a low-rank approximation of this linear operator predicts many known features of incompressible wall-bounded turbulence. In this work, this resolvent model for wall-bounded turbulence is extended to include a passive scalar field. This formulation allows for a number of additional simplifications that reduce model complexity. Firstly, it is shown that the effect of changing scalar diffusivity can be approximated through a transformation of spatial wavenumbers and temporal frequencies. Secondly, passive scalar dynamics may be studied through the low-rank approximation of a passive scalar resolvent operator, which is decoupled from velocity response modes. Thirdly, this passive scalar resolvent operator is amenable to approximation by semi-analytic methods. We investigate the extent to which this resulting hierarchy of models can describe and predict passive scalar dynamics and statistics in wall-bounded turbulence. The support of AFOSR under Grant Numbers FA9550-16-1-0232 and FA9550-16-1-0361 is gratefully acknowledged.

Continuous wave dye-laser technique for simultaneous, spatially resolved measurements of temperature, pressure, and velocity of NO in an underexpanded free jet

NASA Technical Reports Server (NTRS)

Di Rosa, Michael D.; Chang, Albert Y.; Hanson, Ronald K.

1993-01-01

Gas dynamic quantities within an underexpanded nitrogen free jet, seeded with 0.5 percent NO, were measured nonintrusively by using an intracavity-doubled, rapid-tuning, CW ring dye laser. The UV beam passed obliquely through the jet axis, and its frequency repetitively scanned across adjacent rotational lines in the NO gamma band near 225 nm at a rate of 4 kHz. Spatially resolved excitation scans were obtained by monitoring the induced broadband fluoresence. Modeling the Doppler-shifted excitation scans with Voigt profiles permitted simultaneous determinations of NO velocity, rotational temperature, and pressure. Zero Doppler shift was referenced to an absorption trace obtained across a static cell and recorded concurrently with the excitation scan. Typically, the measured and predicted axial distributions agreed within 10 percent. At high Mach numbers there was evidence of rotational freezing of NO.

The Sun at high spatial resolution: The physics of small spatial structures in a magnetized medium

NASA Technical Reports Server (NTRS)

Rosner, R. T.

1986-01-01

An attempt is made to provide a perspective on the problem of spatial structuring on scales smaller than can presently be directly and regularly observed from the ground or with which current space-based instrumentation can be anticipated. There is abundant evidence from both observations and theory that such spatial structuring of the solar outer atmosphere is ubiquitous not only on the observed scales, but also on spatial scales down to (at least) the subarcsecond range. This is not to say that the results to be obtained from observations on these small scales can be anticipated: quite the opposite. What is clear instead is that many of the classic problems of coronal and chromospheric activity - involving the basic dissipative nature of magnetized plasmas - will be seen from a novel perspective at these scales, and that there are reasons for believing that dynamical processes of importance to activity on presently-resolved scales will themselves begin to be resolved on the sub-arcsecond level. Since the Sun is the only astrophysical laboratory for which there is any hope of studying these processes in any detail, this observatioinal opportunity is an exciting prospect for any student of magnetic activity in astrophysics.

Radar-based Quantitative Precipitation Forecasting using Spatial-scale Decomposition Method for Urban Flood Management

NASA Astrophysics Data System (ADS)

Yoon, S.; Lee, B.; Nakakita, E.; Lee, G.

2016-12-01

Recent climate changes and abnormal weather phenomena have resulted in increased occurrences of localized torrential rainfall. Urban areas in Korea have suffered from localized heavy rainfall, including the notable Seoul flood disaster in 2010 and 2011. The urban hydrological environment has changed in relation to precipitation, such as reduced concentration time, a decreased storage rate, and increased peak discharge. These changes have altered and accelerated the severity of damage to urban areas. In order to prevent such urban flash flood damages, we have to secure the lead time for evacuation through the improvement of radar-based quantitative precipitation forecasting (QPF). The purpose of this research is to improve the QPF products using spatial-scale decomposition method for considering the life time of storm and to assess the accuracy between traditional QPF method and proposed method in terms of urban flood management. The layout of this research is as below. First, this research applies the image filtering to separate the spatial-scale of rainfall field. Second, the separated small and large-scale rainfall fields are extrapolated by each different forecasting method. Third, forecasted rainfall fields are combined at each lead time. Finally, results of this method are evaluated and compared with the results of uniform advection model for urban flood modeling. It is expected that urban flood information using improved QPF will help to reduce casualties and property damage caused by urban flooding through this research.

Policy applications of a highly resolved spatial and temporal onroad carbon dioxide emissions data product for the U.S.: Analyses and their implications for mitigation

NASA Astrophysics Data System (ADS)

Mendoza Lebrun, Daniel

Onroad CO2 emissions were analyzed as part of overall GHG emissions, but those studies have suffered from one or more of these five shortcomings: 1) the spatial resolution was coarse, usually encompassing a region, or the entire U.S.; 2) the temporal resolution was coarse (annual or monthly); 3) the study region was limited, usually a metropolitan planning organization (MPO) or state; 4) fuel sales were used as a proxy to quantify fuel consumption instead of focusing on travel; 5) the spatial heterogeneity of fleet and road network composition was not considered and instead national averages are used. Normalized vehicle-type state-level spatial biases range from 2.6% to 8.1%, while the road type classification biases range from -6.3% to 16.8%. These biases are found to cause errors in reduction estimates as large as ±60%, corresponding to ±0.2 MtC, for a national-average emissions mitigation strategy focused on a 10% emissions reduction from a single vehicle class. Temporal analysis shows distinct emissions seasonality that is particularly visible in the northernmost latitudes, demonstrating peak-to-peak deviations from the annual mean of up to 50%. The hourly structure shows peak-to-peak deviation from a weekly average of up to 200% for heavy-duty (HD) vehicles and 140% for light-duty (LD) vehicles. The present study focuses on reduction of travel and fuel economy improvements by putting forth several mitigation scenarios aimed at reducing VMT and increasing vehicle fuel efficiency. It was found that the most effective independent reduction strategies are those that increase fuel efficiency by extending standards proposed by the corporate average fuel economy (CAFE) or reduction of fuel consumption due to price increases. These two strategies show cumulative emissions reductions of approximately 11% and 12%, respectively, from a business as usual (BAU) approach over the 2000-2050 period. The U.S. onroad transportation sector is long overdue a comprehensive study

Timing the formation and assembly of early-type galaxies via spatially resolved stellar populations analysis

NASA Astrophysics Data System (ADS)

Martín-Navarro, Ignacio; Vazdekis, Alexandre; Falcón-Barroso, Jesús; La Barbera, Francesco; Yıldırım, Akın; van de Ven, Glenn

2018-04-01

To investigate star formation and assembly processes of massive galaxies, we present here a spatially resolved stellar population analysis of a sample of 45 elliptical galaxies (Es) selected from the Calar Alto Legacy Integral Field Area survey. We find rather flat age and [Mg/Fe] radial gradients, weakly dependent on the effective velocity dispersion of the galaxy within half-light radius. However, our analysis shows that metallicity gradients become steeper with increasing galaxy velocity dispersion. In addition, we have homogeneously compared the stellar population gradients of our sample of Es to a sample of nearby relic galaxies, i.e. local remnants of the high-z population of red nuggets. This comparison indicates that, first, the cores of present-day massive galaxies were likely formed in gas-rich, rapid star formation events at high redshift (z ≳ 2). This led to radial metallicity variations steeper than observed in the local Universe, and positive [Mg/Fe] gradients. Secondly, our analysis also suggests that a later sequence of minor dry mergers, populating the outskirts of early-type galaxies (ETGs), flattened the pristine [Mg/Fe] and metallicity gradients. Finally, we find a tight age-[Mg/Fe] relation, supporting that the duration of the star formation is the main driver of the [Mg/Fe] enhancement in massive ETGs. However, the star formation time-scale alone is not able to fully explain our [Mg/Fe] measurements. Interestingly, our results match the expected effect that a variable stellar initial mass function would have on the [Mg/Fe] ratio.

Haze and cloud distribution in Uranus' atmosphere based on high-contrast spatially resolved polarization measurements

NASA Astrophysics Data System (ADS)

Kostogryz, Nadiia; Berdyugina, Svetlana; Gisler, Daniel; Berkefeld, Thomas

2017-04-01

In planetary atmospheres, main sources of opacity are molecular absorption and scattering on molecules, hazes and aerosols. Hence, light reflected from a planetary atmosphere can be linearly polarized. Polarization study of inner solar system planets and exoplanets is a powerful method to characterize their atmospheres, because of a wide range of observable phase angles. For outer solar system planets, observable phase angles are very limited. For instance, Uranus can only be observed up to 3.2 degrees away from conjunctions, and its disk-integrated polarization is close to zero due to the back-scattering geometry. However, resolving the disk of Uranus and measuring the center-to-limb polarization can help constraining the vertical atmospheric structure and the nature of scattering aerosols and particles. In October 2016, we carried out polarization measurements of Uranus in narrow-band filters centered at methane bands and the adjacent continuum using the GREGOR Planet Polarimeter (GPP). The GPP is a high-precision polarimeter and is mounted at the 1.5-m GREGOR solar telescope, which is suitable for observing at night. In order to reach a high spatial resolution, the instrument uses an adaptive-optics system of the telescope. To interpret our measurements, we solve the polarized radiative transfer problem taking into account different scattering and absorption opacities. We calculate the center-to-limb variation of polarization of Uranus' disk in the continuum spectrum and in methane bands. By varying the vertical distribution of haze and cloud layers, we derive the vertical structure of the best-fit Uranus atmosphere.

Spatially resolved observation of crystal-face-dependent catalysis by single turnover counting

NASA Astrophysics Data System (ADS)

Roeffaers, Maarten B. J.; Sels, Bert F.; Uji-I, Hiroshi; de Schryver, Frans C.; Jacobs, Pierre A.; de Vos, Dirk E.; Hofkens, Johan

2006-02-01

Catalytic processes on surfaces have long been studied by probing model reactions on single-crystal metal surfaces under high vacuum conditions. Yet the vast majority of industrial heterogeneous catalysis occurs at ambient or elevated pressures using complex materials with crystal faces, edges and defects differing in their catalytic activity. Clearly, if new or improved catalysts are to be rationally designed, we require quantitative correlations between surface features and catalytic activity-ideally obtained under realistic reaction conditions. Transmission electron microscopy and scanning tunnelling microscopy have allowed in situ characterization of catalyst surfaces with atomic resolution, but are limited by the need for low-pressure conditions and conductive surfaces, respectively. Sum frequency generation spectroscopy can identify vibrations of adsorbed reactants and products in both gaseous and condensed phases, but so far lacks sensitivity down to the single molecule level. Here we adapt real-time monitoring of the chemical transformation of individual organic molecules by fluorescence microscopy to monitor reactions catalysed by crystals of a layered double hydroxide immersed in reagent solution. By using a wide field microscope, we are able to map the spatial distribution of catalytic activity over the entire crystal by counting single turnover events. We find that ester hydrolysis proceeds on the lateral {1010} crystal faces, while transesterification occurs on the entire outer crystal surface. Because the method operates at ambient temperature and pressure and in a condensed phase, it can be applied to the growing number of liquid-phase industrial organic transformations to localize catalytic activity on and in inorganic solids. An exciting opportunity is the use of probe molecules with different size and functionality, which should provide insight into shape-selective or structure-sensitive catalysis and thus help with the rational design of new or

Spatially unresolved SED fitting can underestimate galaxy masses: a solution to the missing mass problem

NASA Astrophysics Data System (ADS)

Sorba, Robert; Sawicki, Marcin

2018-05-01

We perform spatially resolved, pixel-by-pixel Spectral Energy Distribution (SED) fitting on galaxies up to z ˜ 2.5 in the Hubble eXtreme Deep Field (XDF). Comparing stellar mass estimates from spatially resolved and spatially unresolved photometry we find that unresolved masses can be systematically underestimated by factors of up to 5. The ratio of the unresolved to resolved mass measurement depends on the galaxy's specific star formation rate (sSFR): at low sSFRs the bias is small, but above sSFR ˜ 10-9.5 yr-1 the discrepancy increases rapidly such that galaxies with sSFRs ˜ 10-8 yr-1 have unresolved mass estimates of only one-half to one-fifth of the resolved value. This result indicates that stellar masses estimated from spatially unresolved data sets need to be systematically corrected, in some cases by large amounts, and we provide an analytic prescription for applying this correction. We show that correcting stellar mass measurements for this bias changes the normalization and slope of the star-forming main sequence and reduces its intrinsic width; most dramatically, correcting for the mass bias increases the stellar mass density of the Universe at high redshift and can resolve the long-standing discrepancy between the directly measured cosmic SFR density at z ≳ 1 and that inferred from stellar mass densities (`the missing mass problem').

Depth resolved hyperspectral imaging spectrometer based on structured light illumination and Fourier transform interferometry

PubMed Central

Choi, Heejin; Wadduwage, Dushan; Matsudaira, Paul T.; So, Peter T.C.

2014-01-01

A depth resolved hyperspectral imaging spectrometer can provide depth resolved imaging both in the spatial and the spectral domain. Images acquired through a standard imaging Fourier transform spectrometer do not have the depth-resolution. By post processing the spectral cubes (x, y, λ) obtained through a Sagnac interferometer under uniform illumination and structured illumination, spectrally resolved images with depth resolution can be recovered using structured light illumination algorithms such as the HiLo method. The proposed scheme is validated with in vitro specimens including fluorescent solution and fluorescent beads with known spectra. The system is further demonstrated in quantifying spectra from 3D resolved features in biological specimens. The system has demonstrated depth resolution of 1.8 μm and spectral resolution of 7 nm respectively. PMID:25360367

Quantitative evaluation of spatial scale of carrier trapping at grain boundary by GHz-microwave dielectric loss spectroscopy

NASA Astrophysics Data System (ADS)

Choi, W.; Tsutsui, Y.; Miyakai, T.; Sakurai, T.; Seki, S.

2017-11-01

Charge carrier mobility is an important primary parameter for the electronic conductive materials, and the intrinsic limit of the mobility has been hardly access by conventional direct-current evaluation methods. In the present study, intra-grain hole mobility of pentacene thin films was estimated quantitatively using microwave-based dielectric loss spectroscopy (time-resolved microwave conductivity measurement) in alternating current mode of charge carrier local motion. Metal-insulator-semiconductor devices were prepared with different insulating polymers or substrate temperature upon vacuum deposition of the pentacene layer, which afforded totally four different grain-size conditions of pentacene layers. Under the condition where the local motion was determined by interfacial traps at the pentacene grain boundaries (grain-grain interfaces), the observed hole mobilities were plotted against the grain sizes, giving an excellent correlation fit successfully by a parabolic function representative of the boarder length. Consequently, the intra-grain mobility and trap-release time of holes were estimated as 15 cm2 V-1 s-1 and 9.4 ps.

Qualitative Research? Quantitative Research? What's the Problem? Resolving the Dilemma via a Postconstructivist Approach.

ERIC Educational Resources Information Center

Shank, Gary

It is argued that the debate between qualitative and quantitative research for educational researchers is actually an argument between constructivism and positivism. Positivism has been the basis for most quantitative research in education. Two different things are actually meant when constructivism is discussed (constructivism and…

Energy-resolved neutron imaging for inertial confinement fusion

NASA Astrophysics Data System (ADS)

Moran, M. J.; Haan, S. W.; Hatchett, S. P.; Izumi, N.; Koch, J. A.; Lerche, R. A.; Phillips, T. W.

2003-03-01

The success of the National Ignition Facility program will depend on diagnostic measurements which study the performance of inertial confinement fusion (ICF) experiments. Neutron yield, fusion-burn time history, and images are examples of important diagnostics. Neutron and x-ray images will record the geometries of compressed targets during the fusion-burn process. Such images provide a critical test of the accuracy of numerical modeling of ICF experiments. They also can provide valuable information in cases where experiments produce unexpected results. Although x-ray and neutron images provide similar data, they do have significant differences. X-ray images represent the distribution of high-temperature regions where fusion occurs, while neutron images directly reveal the spatial distribution of fusion-neutron emission. X-ray imaging has the advantage of a relatively straightforward path to the imaging system design. Neutron imaging, by using energy-resolved detection, offers the intriguing advantage of being able to provide independent images of burning and nonburning regions of the nuclear fuel. The usefulness of energy-resolved neutron imaging depends on both the information content of the data and on the quality of the data that can be recorded. The information content will relate to the characteristic neutron spectra that are associated with emission from different regions of the source. Numerical modeling of ICF fusion burn will be required to interpret the corresponding energy-dependent images. The exercise will be useful only if the images can be recorded with sufficient definition to reveal the spatial and energy-dependent features of interest. Several options are being evaluated with respect to the feasibility of providing the desired simultaneous spatial and energy resolution.

Concept of proton radiography using energy resolved dose measurement.

PubMed

Bentefour, El H; Schnuerer, Roland; Lu, Hsiao-Ming

2016-08-21

Energy resolved dosimetry offers a potential path to single detector based proton imaging using scanned proton beams. This is because energy resolved dose functions encrypt the radiological depth at which the measurements are made. When a set of predetermined proton beams 'proton imaging field' are used to deliver a well determined dose distribution in a specific volume, then, at any given depth x of this volume, the behavior of the dose against the energies of the proton imaging field is unique and characterizes the depth x. This concept applies directly to proton therapy scanning delivery methods (pencil beam scanning and uniform scanning) and it can be extended to the proton therapy passive delivery methods (single and double scattering) if the delivery of the irradiation is time-controlled with a known time-energy relationship. To derive the water equivalent path length (WEPL) from the energy resolved dose measurement, one may proceed in two different ways. A first method is by matching the measured energy resolved dose function to a pre-established calibration database of the behavior of the energy resolved dose in water, measured over the entire range of radiological depths with at least 1 mm spatial resolution. This calibration database can also be made specific to the patient if computed using the patient x-CT data. A second method to determine the WEPL is by using the empirical relationships between the WEPL and the integral dose or the depth at 80% of the proximal fall off of the energy resolved dose functions in water. In this note, we establish the evidence of the fundamental relationship between the energy resolved dose and the WEPL at the depth of the measurement. Then, we illustrate this relationship with experimental data and discuss its imaging dynamic range for 230 MeV protons.

Spatially-resolved HPGe Gamma-ray Spectroscopy of Swipe Samples

DOE Office of Scientific and Technical Information (OSTI.GOV)

McDonald, Benjamin S.; VanDevender, Brent A.; Wood, Lynn S.

Measurement of swipe samples is a critical element of the National Technical Nuclear Forensics (NTNF) mission. A unique, portable, germanium gamma imager (GeGI-s) from PHDS Co may provide complementary information to current techniques for swipe sample screening. The GeGI-s is a modified version of the commercial GeGI-4, a planar HPGe detector, capable of several million counts per second across the whole detector. The GeGI-s detector is a prototype of a commercial off-the-shelf high rate GeGI. The high rate capability allows high-activity samples be placed directly on the face of the detector. Utilizing the high energy resolution and pixelization of themore » detector, the GeGI-s can generate isotope specific spatial maps of the materials on the swipe sample. To prove this technology is viable for such mapping, the GeGI-s detector response to spatially distributed events must be well characterized. The detection efficiency as a function of location has been characterized to understand the non-uniformities presented as a collimated photon beam was rastered vertically and horizontally across the face of the detector. The detection efficiency as a function of location has been characterized to understand the non-uniformities presented as a collimated photon beam was rastered vertically and horizontally across the face of the detector. The response was found to be primarily uniform and symmetric, however two causes of non-uniformity were found. Both of these causes can ultimately be corrected for in off-line data analysis.« less

The Spatial Distribution of Resolved Young Stars in Blue Compact Dwarf Galaxies

NASA Astrophysics Data System (ADS)

Murphy, K.; Crone, M. M.

2002-12-01

We present the first results from a survey of the distribution of resolved young stars in Blue Compact Dwarf Galaxies. In order to identify the dominant physical processes driving star formation in these puzzling galaxies, we use a multi-scale cluster-finding algorithm to quantify the characteristic scales and properties of star-forming regions, from sizes smaller than 10 pc up to the size of each entire galaxy. This project was partially funded by the Lubin Chair at Skidmore College.

Highly Accurate Quantitative Analysis Of Enantiomeric Mixtures from Spatially Frequency Encoded 1H NMR Spectra.

PubMed

Plainchont, Bertrand; Pitoux, Daisy; Cyrille, Mathieu; Giraud, Nicolas

2018-02-06

We propose an original concept to measure accurately enantiomeric excesses on proton NMR spectra, which combines high-resolution techniques based on a spatial encoding of the sample, with the use of optically active weakly orienting solvents. We show that it is possible to simulate accurately dipolar edited spectra of enantiomers dissolved in a chiral liquid crystalline phase, and to use these simulations to calibrate integrations that can be measured on experimental data, in order to perform a quantitative chiral analysis. This approach is demonstrated on a chemical intermediate for which optical purity is an essential criterion. We find that there is a very good correlation between the experimental and calculated integration ratios extracted from G-SERF spectra, which paves the way to a general method of determination of enantiomeric excesses based on the observation of 1 H nuclei.

Spatially resolved texture and microstructure evolution of additively manufactured and gas gun deformed 304L stainless steel investigated by neutron diffraction and electron backscatter diffraction

DOE PAGES

Takajo, Shigehiro; Brown, Donald William; Clausen, Bjorn; ...

2018-04-30

In this study, we report the characterization of a 304L stainless steel cylindrical projectile produced by additive manufacturing. The projectile was compressively deformed using a Taylor Anvil Gas Gun, leading to a huge strain gradient along the axis of the deformed cylinder. Spatially resolved neutron diffraction measurements on the HIgh Pressure Preferred Orientation time-of-flight diffractometer (HIPPO) and Spectrometer for Materials Research at Temperature and Stress diffractometer (SMARTS) beamlines at the Los Alamos Neutron Science CEnter (LANSCE) with Rietveld and single-peak analysis were used to quantitatively evaluate the volume fractions of the α, γ, and ε phases as well as residualmore » strain and texture. The texture of the γ phase is consistent with uniaxial compression, while the α texture can be explained by the Kurdjumov–Sachs relationship from the γ texture after deformation. This indicates that the material first deformed in the γ phase and subsequently transformed at larger strains. The ε phase was only found in volumes close to the undeformed material with a texture connected to the γ texture by the Shoji–Nishiyama orientation relationship. This allows us to conclude that the ε phase occurs as an intermediate phase at lower strain, and is superseded by the α phase when strain increases further. We found a proportionality between the root-mean-squared microstrain of the γ phase, dominated by the dislocation density, with the α volume fraction, consistent with strain-induced martensite α formation. In conclusion, knowledge of the sample volume with the ε phase from the neutron diffraction analysis allowed us to identify the ε phase by electron back scatter diffraction analysis, complementing the neutron diffraction analysis with characterization on the grain level.« less

Spatially resolved texture and microstructure evolution of additively manufactured and gas gun deformed 304L stainless steel investigated by neutron diffraction and electron backscatter diffraction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Takajo, Shigehiro; Brown, Donald William; Clausen, Bjorn

In this study, we report the characterization of a 304L stainless steel cylindrical projectile produced by additive manufacturing. The projectile was compressively deformed using a Taylor Anvil Gas Gun, leading to a huge strain gradient along the axis of the deformed cylinder. Spatially resolved neutron diffraction measurements on the HIgh Pressure Preferred Orientation time-of-flight diffractometer (HIPPO) and Spectrometer for Materials Research at Temperature and Stress diffractometer (SMARTS) beamlines at the Los Alamos Neutron Science CEnter (LANSCE) with Rietveld and single-peak analysis were used to quantitatively evaluate the volume fractions of the α, γ, and ε phases as well as residualmore » strain and texture. The texture of the γ phase is consistent with uniaxial compression, while the α texture can be explained by the Kurdjumov–Sachs relationship from the γ texture after deformation. This indicates that the material first deformed in the γ phase and subsequently transformed at larger strains. The ε phase was only found in volumes close to the undeformed material with a texture connected to the γ texture by the Shoji–Nishiyama orientation relationship. This allows us to conclude that the ε phase occurs as an intermediate phase at lower strain, and is superseded by the α phase when strain increases further. We found a proportionality between the root-mean-squared microstrain of the γ phase, dominated by the dislocation density, with the α volume fraction, consistent with strain-induced martensite α formation. In conclusion, knowledge of the sample volume with the ε phase from the neutron diffraction analysis allowed us to identify the ε phase by electron back scatter diffraction analysis, complementing the neutron diffraction analysis with characterization on the grain level.« less

Probing Minor-merger-driven Star Formation In Early-type Galaxies Using Spatially-resolved Spectro-photometric Studies

NASA Astrophysics Data System (ADS)

Kaviraj, Sugata; Crockett, M.; Silk, J.; O'Connell, R. W.; Whitmore, B.; Windhorst, R.; Cappellari, M.; Bureau, M.; Davies, R.

2012-01-01

Recent studies that leverage the rest-frame ultraviolet (UV) spectrum have revealed widespread recent star formation in early-type galaxies (ETGs), traditionally considered to be old, passively-evolving systems. This recent star formation builds 20% of the ETG stellar mass after z 1, driven by repeated minor mergers between ETGs and small, gas-rich satellites. We demonstrate how spatially-resolved studies, using a combination of high-resolution UV-optical imaging and integral-field spectroscopy (IFS), is a powerful tool to quantify the assembly history of individual ETGs and elucidate the poorly-understood minor-merger process. Using a combination of WFC3 UV-optical (2500-8200 angstroms) imaging and IFS from the SAURON project of the ETG NGC 4150, we show that this galaxy experienced a merger with mass ratio 1:15 around 0.9 Gyr ago, which formed 3% of its stellar mass and a young kinematically-decoupled core. A UV-optical analysis of its globular cluster system shows that the bulk of the stars locked up in these clusters likely formed 6-7 Gyrs in the past. We introduce a new HST-WFC3 programme, approved in Cycle 19, which will leverage similar UV-optical imaging of a representative sample of nearby ETGs from SAURON to study the recent star formation and its drivers in unprecedented detail and put definitive constraints on minor-merger-driven star formation in massive galaxies at late epochs.

Spatially resolved analysis of plutonium isotopic signatures in environmental particle samples by laser ablation-MC-ICP-MS.

PubMed

Konegger-Kappel, Stefanie; Prohaska, Thomas

2016-01-01

Laser ablation-multi-collector-inductively coupled plasma mass spectrometry (LA-MC-ICP-MS) was optimized and investigated with respect to its performance for determining spatially resolved Pu isotopic signatures within radioactive fuel particle clusters. Fuel particles had been emitted from the Chernobyl nuclear power plant (ChNPP) where the 1986 accident occurred and were deposited in the surrounding soil, where weathering processes caused their transformation into radioactive clusters, so-called micro-samples. The size of the investigated micro-samples, which showed surface alpha activities below 40 mBq, ranged from about 200 to 1000 μm. Direct single static point ablations allowed to identify variations of Pu isotopic signatures not only between distinct fuel particle clusters but also within individual clusters. The resolution was limited to 100 to 120 μm as a result of the applied laser ablation spot sizes and the resolving power of the nuclear track radiography methodology that was applied for particle pre-selection. The determined (242)Pu/(239)Pu and (240)Pu/(239)Pu isotope ratios showed a variation from low to high Pu isotope ratios, ranging from 0.007(2) to 0.047(8) for (242)Pu/(239)Pu and from 0.183(13) to 0.577(40) for (240)Pu/(239)Pu. In contrast to other studies, the applied methodology allowed for the first time to display the Pu isotopic distribution in the Chernobyl fallout, which reflects the differences in the spent fuel composition over the reactor core. The measured Pu isotopic signatures are in good agreement with the expected Pu isotopic composition distribution that is typical for a RBMK-1000 reactor, indicating that the analyzed samples are originating from the ill-fated Chernobyl reactor. The average Pu isotope ratios [(240)Pu/(239)Pu = 0.388(86), (242)Pu/(239)Pu = 0.028(11)] that were calculated from all investigated samples (n = 48) correspond well to previously published results of Pu analyses in contaminated samples from

The quantitative evaluation of cholinergic markers in spatial memory improvement induced by nicotine-bucladesine combination in rats.

PubMed

Azami, Kian; Etminani, Maryam; Tabrizian, Kaveh; Salar, Fatemeh; Belaran, Maryam; Hosseini, Asieh; Hosseini-Sharifabad, Ali; Sharifzadeh, Mohammad

2010-06-25

We previously showed that post-training intra-hippocampal infusion of nicotine-bucladesine combination enhanced spatial memory retention in the Morris water maze. Here we investigated the role of cholinergic markers in nicotine-bucladesine combination-induced memory improvement. We assessed the expression of choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) in CA1 region of the hippocampus and medial septal area (MSA) of the brain. Post-training bilateral infusion of a low concentration of either nicotine or bucladesine into the CA1 region of the hippocampus did not affect spatial memory significantly. Quantitative immunostaining analysis of optical density in CA1 regions and evaluation of immunopositive neurons in medial septal area of brain sections from all combination groups revealed a significant increase (P<0.001) in the ChAT and VAChT immunoreactivity. The maximum increase was observed with combination of 10-microM/side bucladesine and 0.5 microg/side nicotine and in a concentration dependent manner. Also, increase in the optical density and amount of ChAT and VAChT immunostaining correlated with the decrease in escape latency and traveled distance in rats treated with nicotine and low dose of bucladesine. Taken together, these results suggest that significant increases of ChAT and VAChT protein expressions in the CA1 region and medial septal area are the possible mechanisms of spatial memory improvement induced by nicotine-bucladesine combination. (c) 2010 Elsevier B.V. All rights reserved.

Non-Destructive Study of Bulk Crystallinity and Elemental Composition of Natural Gold Single Crystal Samples by Energy-Resolved Neutron Imaging

PubMed Central

Tremsin, Anton S.; Rakovan, John; Shinohara, Takenao; Kockelmann, Winfried; Losko, Adrian S.; Vogel, Sven C.

2017-01-01

Energy-resolved neutron imaging enables non-destructive analyses of bulk structure and elemental composition, which can be resolved with high spatial resolution at bright pulsed spallation neutron sources due to recent developments and improvements of neutron counting detectors. This technique, suitable for many applications, is demonstrated here with a specific study of ~5–10 mm thick natural gold samples. Through the analysis of neutron absorption resonances the spatial distribution of palladium (with average elemental concentration of ~0.4 atom% and ~5 atom%) is mapped within the gold samples. At the same time, the analysis of coherent neutron scattering in the thermal and cold energy regimes reveals which samples have a single-crystalline bulk structure through the entire sample volume. A spatially resolved analysis is possible because neutron transmission spectra are measured simultaneously on each detector pixel in the epithermal, thermal and cold energy ranges. With a pixel size of 55 μm and a detector-area of 512 by 512 pixels, a total of 262,144 neutron transmission spectra are measured concurrently. The results of our experiments indicate that high resolution energy-resolved neutron imaging is a very attractive analytical technique in cases where other conventional non-destructive methods are ineffective due to sample opacity. PMID:28102285

Validating crash locations for quantitative spatial analysis: a GIS-based approach.

PubMed

Loo, Becky P Y

2006-09-01

In this paper, the spatial variables of the crash database in Hong Kong from 1993 to 2004 are validated. The proposed spatial data validation system makes use of three databases (the crash, road network and district board databases) and relies on GIS to carry out most of the validation steps so that the human resource required for manually checking the accuracy of the spatial data can be enormously reduced. With the GIS-based spatial data validation system, it was found that about 65-80% of the police crash records from 1993 to 2004 had correct road names and district board information. In 2004, the police crash database contained about 12.7% mistakes for road names and 9.7% mistakes for district boards. The situation was broadly comparable to the United Kingdom. However, the results also suggest that safety researchers should carefully validate spatial data in the crash database before scientific analysis.

A mid-IR interferometric survey with MIDI/VLTI: resolving the second-generation protoplanetary disks around post-AGB binaries

NASA Astrophysics Data System (ADS)

Hillen, M.; Van Winckel, H.; Menu, J.; Manick, R.; Debosscher, J.; Min, M.; de Wit, W.-J.; Verhoelst, T.; Kamath, D.; Waters, L. B. F. M.

2017-03-01

Aims: We present a mid-IR interferometric survey of the circumstellar environment of a specific class of post-asymptotic giant branch (post-AGB) binaries. For this class the presence of a compact dusty disk has been postulated on the basis of various spatially unresolved measurements. The aim is to determine the angular extent of the N-band emission directly and to resolve the compact circumstellar structures. Methods: Our interferometric survey was performed with the MIDI instrument on the VLTI. In total 19 different systems were observed using variable baseline configurations. Combining all the visibilities at a single wavelength at 10.7 μm, we fitted two parametric models to the data: a uniform disk and a ring model mimicking a temperature gradient. We compared our observables of the whole sample, with synthetic data computed from a grid of radiative transfer models of passively irradiated disks in hydrostatic equilibrium. These models are computed with a Monte Carlo code that has been widely applied to describe the structure of protoplanetary disks around young stellar objects (YSO). Results: The spatially resolved observations show that the majority of our targets cluster closely together in the distance-independent size-colour diagram, and have extremely compact N-band emission regions. The typical uniform disk diameter of the N-band emission region is 40 mas, which corresponds to a typical brightness temperature of 400-600 K. The resolved objects display very similar characteristics in the interferometric observables and in the spectral energy distributions. Therefore, the physical properties of the disks around our targets must be similar. Our results are discussed in the light of recently published sample studies of YSOs to compare quantitatively the secondary discs around post-AGB stars to the ones around YSOs. Conclusions: Our high-angular-resolution survey further confirms the disk nature of the circumstellar structures present around wide post

Quantitative dispersion microscopy

PubMed Central

Fu, Dan; Choi, Wonshik; Sung, Yongjin; Yaqoob, Zahid; Dasari, Ramachandra R.; Feld, Michael

2010-01-01

Refractive index dispersion is an intrinsic optical property and a useful source of contrast in biological imaging studies. In this report, we present the first dispersion phase imaging of living eukaryotic cells. We have developed quantitative dispersion microscopy based on the principle of quantitative phase microscopy. The dual-wavelength quantitative phase microscope makes phase measurements at 310 nm and 400 nm wavelengths to quantify dispersion (refractive index increment ratio) of live cells. The measured dispersion of living HeLa cells is found to be around 1.088, which agrees well with that measured directly for protein solutions using total internal reflection. This technique, together with the dry mass and morphology measurements provided by quantitative phase microscopy, could prove to be a useful tool for distinguishing different types of biomaterials and studying spatial inhomogeneities of biological samples. PMID:21113234

Quantitative X-ray Differential Interference Contrast Microscopy

NASA Astrophysics Data System (ADS)

Nakamura, Takashi

Full-field soft x-ray microscopes are widely used in many fields of sciences. Advances in nanofabrication technology enabled short wavelength focusing elements with significantly improved spatial resolution. In the soft x-ray spectral region, samples as small as 12 nm can be resolved using micro zone-plates as the objective lens. In addition to conventional x-ray microscopy in which x-ray absorption difference provides the image contrast, phase contrast mechanisms such as differential phase contrast (DIC) and Zernike phase contrast have also been demonstrated These phase contrast imaging mechanisms are especially attractive at the x-ray wavelengths where phase contrast of most materials is typically 10 times stronger than the absorption contrast. With recent progresses in plasma-based x- ray sources and increasing accessibility to synchrotron user facilities, x-ray microscopes are quickly becoming standard measurement equipment in the laboratory. To further the usefulness of x-ray DIC microscopy this thesis explicitly addresses three known issues with this imaging modality by introducing new techniques and devices First, as opposed to its visible-light counterpart, no quantitative phase imaging technique exists for x-ray DIC microscopy. To address this issue, two nanoscale x-ray quantitative phase imaging techniques, using exclusive OR (XOR) patterns and zone-plate doublets, respectively, are proposed. Unlike existing x-ray quantitative phase imaging techniques such as Talbot interferometry and ptychography, no dedicated experimental setups or stringent illumination coherence are needed for quantitative phase retrieval. Second, to the best of our knowledge, no quantitative performance characterization of DIC microscopy exists to date. Therefore the imaging system's response to sample's spatial frequency is not known In order to gain in-depth understanding of this imaging modality, performance of x-ray DIC microscopy is quantified using modulation transfer function

Fiber optic sensors for sub-centimeter spatially resolved measurements: Review and biomedical applications

NASA Astrophysics Data System (ADS)

Tosi, Daniele; Schena, Emiliano; Molardi, Carlo; Korganbayev, Sanzhar

2018-07-01

One of the current frontier of optical fiber sensors, and a unique asset of this sensing technology is the possibility to use a whole optical fiber, or optical fiber device, as a sensor. This solution allows shifting the whole sensing paradigm, from the measurement of a single physical parameter (such as temperature, strain, vibrations, pressure) to the measurement of a spatial distribution, or profiling, of a physical parameter along the fiber length. In the recent years, several technologies are achieving this task with unprecedentedly narrow spatial resolution, ranging from the sub-millimeter to the centimeter-level. In this work, we review the main fiber optic sensing technologies that achieve a narrow spatial resolution: Fiber Bragg Grating (FBG) dense arrays, chirped FBG (CFBG) sensors, optical frequency domain reflectometry (OFDR) based on either Rayleigh scattering or reflective elements, and microwave photonics (MWP). In the second part of the work, we present the impact of spatially dense fiber optic sensors in biomedical applications, where they find the main impact, presenting the key results obtained in thermo-therapies monitoring, high-resolution diagnostic, catheters monitoring, smart textiles, and other emerging applicative fields.

Fast time-resolved aerosol collector: proof of concept

NASA Astrophysics Data System (ADS)

Yu, X.-Y.; Cowin, J. P.; Iedema, M. J.; Ali, H.

2010-10-01

Atmospheric particles can be collected in the field on substrates for subsequent laboratory analysis via chemically sensitive single particle methods such as scanning electron microscopy with energy dispersive x-ray analysis. With moving substrates time resolution of seconds to minutes can be achieved. In this paper, we demonstrate how to increase the time resolution when collecting particles on a substrate to a few milliseconds to provide real-time information. Our fast time-resolved aerosol collector ("Fast-TRAC") microscopically observes the particle collection on a substrate and records an on-line video. Particle arrivals are resolved to within a single frame (4-17 ms in this setup), and the spatial locations are matched to the subsequent single particle analysis. This approach also provides in-situ information on particle size and number concentration. Applications are expected in airborne studies of cloud microstructure, pollution plumes, and surface long-term monitoring.

Fast time-resolved aerosol collector: proof of concept

NASA Astrophysics Data System (ADS)

Yu, X.-Y.; Cowin, J. P.; Iedema, M. J.; Ali, H.

2010-06-01

Atmospheric particles can be collected in the field on substrates for subsequent laboratory analysis via chemically sensitive single particle methods such as scanning electron microscopy with energy dispersive x-ray analysis. With moving substrates time resolution of seconds to minutes can be achieved. In this paper, we demonstrate how to increase the time resolution when collecting particles on a substrate to a few milliseconds to provide real-time information. Our fast time-resolved aerosol collector ("Fast-TRAC") microscopically observes the particle collection on a substrate and records an on-line video. Particle arrivals are resolved to within a single frame (4-17 ms in this setup), and the spatial locations are matched to the subsequent single particle analysis. This approach also provides in-situ information on particle size and number concentration. Applications are expected in airborne studies of cloud microstructure, pollution plumes, and surface long-term monitoring.

Continuous-flow liquid microjunction surface sampling probe connected on-line with high-performance liquid chromatography/mass spectrometry for spatially resolved analysis of small molecules and proteins.

PubMed

Van Berkel, Gary J; Kertesz, Vilmos

2013-06-30

A continuous-flow liquid microjunction surface sampling probe extracts soluble material from surfaces for direct ionization and detection by mass spectrometry. Demonstrated here is the on-line coupling of such a probe with high-performance liquid chromatography/mass spectrometry (HPLC/MS) enabling extraction, separation and detection of small molecules and proteins from surfaces in a spatially resolved (~0.5 mm diameter spots) manner. A continuous-flow liquid microjunction surface sampling probe was connected to a six-port, two-position valve for extract collection and injection to an HPLC column. A QTRAP® 5500 hybrid triple quadrupole linear ion trap equipped with a Turbo V™ ion source operated in positive electrospray ionization (ESI) mode was used for all experiments. The system operation was tested with the extraction, separation and detection of propranolol and associated metabolites from drug dosed tissues, caffeine from a coffee bean, cocaine from paper currency, and proteins from dried sheep blood spots on paper. Confirmed in the tissue were the parent drug and two different hydroxypropranolol glucuronides. The mass spectrometric response for these compounds from different locations in the liver showed an increase with increasing extraction time (5, 20 and 40 s). For on-line separation and detection/identification of extracted proteins from dried sheep blood spots, two major protein peaks dominated the chromatogram and could be correlated with the expected masses for the hemoglobin α and β chains. Spatially resolved sampling, separation, and detection of small molecules and proteins from surfaces can be accomplished using a continuous-flow liquid microjunction surface sampling probe coupled on-line with HPLC/MS detection. Published in 2013. This article is a U.S. Government work and is in the public domain in the USA.

Optical filter highlighting spectral features part II: quantitative measurements of cosmetic foundation and assessment of their spatial distributions under realistic facial conditions.

PubMed

Nishino, Ken; Nakamura, Mutsuko; Matsumoto, Masayuki; Tanno, Osamu; Nakauchi, Shigeki

2011-03-28

We previously proposed a filter that could detect cosmetic foundations with high discrimination accuracy [Opt. Express 19, 6020 (2011)]. This study extends the filter's functionality to the quantification of the amount of foundation and applies the filter for the assessment of spatial distributions of foundation under realistic facial conditions. Human faces that are applied with quantitatively controlled amounts of cosmetic foundations were measured using the filter. A calibration curve between pixel values of the image and the amount of foundation was created. The optical filter was applied to visualize spatial foundation distributions under realistic facial conditions, which clearly indicated areas on the face where foundation remained even after cleansing. Results confirm that the proposed filter could visualize and nondestructively inspect the foundation distributions.

Beyond Conceptual Knowledge: The Impact of Children’s Theory-of-Mind on Dyadic Spatial Tasks

PubMed Central

Viana, Karine M. P.; Zambrana, Imac M.; Karevold, Evalill B.; Pons, Francisco

2016-01-01

Recent studies show that Theory of Mind (ToM) has implications for children’s social competences and psychological well-being. Nevertheless, although it is well documented that children overall take advantage when they have to resolve cognitive problems together with a partner, whether individual difference in ToM is one of the mechanisms that could explain cognitive performances produced in social interaction has received little attention. This study examines to what extent ToM explains children’s spatial performances in a dyadic situation. The sample includes 66 boys and girls between the ages of 5–9 years, who were tested for their ToM and for their competence to resolve a Spatial task involving mental rotation and spatial perspective taking, first individually and then in a dyadic condition. Results showed, in accordance with previous research, that children performed better on the Spatial task when they resolved it with a partner. Specifically, children’s ToM was a better predictor of their spatial performances in the dyadic condition than their age, gender, and spatial performances in the individual setting. The findings are discussed in terms of the relation between having a conceptual understanding of the mind and the practical implications of this knowledge for cognitive performances in social interaction regarding mental rotation and spatial perspective taking. PMID:27812344

Resolving power of diffraction imaging with an objective: a numerical study.

PubMed

Wang, Wenjin; Liu, Jing; Lu, Jun Qing; Ding, Junhua; Hu, Xin-Hua

2017-05-01

Diffraction imaging in the far-field can detect 3D morphological features of an object for its coherent nature. We describe methods for accurate calculation and analysis of diffraction images of scatterers of single and double spheres by an imaging unit based on microscope objective at non-conjugate positions. A quantitative study of the calculated diffraction imaging in spectral domain has been performed to assess the resolving power of diffraction imaging. It has been shown numerically that with coherent illumination of 532 nm in wavelength the imaging unit can resolve single spheres of 2 μm or larger in diameters and double spheres separated by less than 300 nm between their centers.

Dynamic inundation mapping of Hurricane Harvey flooding in the Houston metro area using hyper-resolution modeling and quantitative image reanalysis

NASA Astrophysics Data System (ADS)

Noh, S. J.; Lee, J. H.; Lee, S.; Zhang, Y.; Seo, D. J.

2017-12-01

Hurricane Harvey was one of the most extreme weather events in Texas history and left significant damages in the Houston and adjoining coastal areas. To understand better the relative impact to urban flooding of extreme amount and spatial extent of rainfall, unique geography, land use and storm surge, high-resolution water modeling is necessary such that natural and man-made components are fully resolved. In this presentation, we reconstruct spatiotemporal evolution of inundation during Hurricane Harvey using hyper-resolution modeling and quantitative image reanalysis. The two-dimensional urban flood model used is based on dynamic wave approximation and 10 m-resolution terrain data, and is forced by the radar-based multisensor quantitative precipitation estimates. The model domain includes Buffalo, Brays, Greens and White Oak Bayous in Houston. The model is simulated using hybrid parallel computing. To evaluate dynamic inundation mapping, we combine various qualitative crowdsourced images and video footages with LiDAR-based terrain data.

Spatially Resolved Imaging at 350 Micrometers of Cold Dust in Nearby Elliptical Galaxies

NASA Technical Reports Server (NTRS)

Leeuw, Lerothodi L.; Davidson, Jacqueline; Dowell, C. Darren; Matthews, Henry E.

2008-01-01

Continuum observations at 350 micrometers of seven nearby elliptical galaxies for which CO gas disks have recently been resolved with interferometry mapping are presented. These SHARC II mapping results provide the first clearly resolved far-infrared (FIR)-to-submillimeter continuum emission from cold dust (with temperatures 31 K is approximately greater than T approximately greater than 23 K) of any elliptical galaxy at a distance greater than 40 Mpc. The measured FIR excess shows that the most likely and dominant heating source of this dust is not dilute stellar radiation or cooling flows, but rather star formation that could have been triggered by an accretion or merger event and fueled by dust-rich material that has settled in a dense region cospatial with the central CO gas disks. The dust is detected even in two cluster ellipticals that are deficient in H (sub I), showing that, unlike H (sub I), cold dust and CO in ellipticals can survive in the presence of hot X-ray gas, even in galaxy clusters. No dust cooler than 20 K, either distributed outside the CO disks or cospatial with and heated by the entire dilute stellar optical galaxy (or very extended H (sub I)), is currently evident.

Quantitative secondary electron imaging for work function extraction at atomic level and layer identification of graphene

PubMed Central

Zhou, Yangbo; Fox, Daniel S; Maguire, Pierce; O’Connell, Robert; Masters, Robert; Rodenburg, Cornelia; Wu, Hanchun; Dapor, Maurizio; Chen, Ying; Zhang, Hongzhou

2016-01-01

Two-dimensional (2D) materials usually have a layer-dependent work function, which require fast and accurate detection for the evaluation of their device performance. A detection technique with high throughput and high spatial resolution has not yet been explored. Using a scanning electron microscope, we have developed and implemented a quantitative analytical technique which allows effective extraction of the work function of graphene. This technique uses the secondary electron contrast and has nanometre-resolved layer information. The measurement of few-layer graphene flakes shows the variation of work function between graphene layers with a precision of less than 10 meV. It is expected that this technique will prove extremely useful for researchers in a broad range of fields due to its revolutionary throughput and accuracy. PMID:26878907

RESOLVE: A new algorithm for aperture synthesis imaging of extended emission in radio astronomy

NASA Astrophysics Data System (ADS)

Junklewitz, H.; Bell, M. R.; Selig, M.; Enßlin, T. A.

2016-02-01

We present resolve, a new algorithm for radio aperture synthesis imaging of extended and diffuse emission in total intensity. The algorithm is derived using Bayesian statistical inference techniques, estimating the surface brightness in the sky assuming a priori log-normal statistics. resolve estimates the measured sky brightness in total intensity, and the spatial correlation structure in the sky, which is used to guide the algorithm to an optimal reconstruction of extended and diffuse sources. During this process, the algorithm succeeds in deconvolving the effects of the radio interferometric point spread function. Additionally, resolve provides a map with an uncertainty estimate of the reconstructed surface brightness. Furthermore, with resolve we introduce a new, optimal visibility weighting scheme that can be viewed as an extension to robust weighting. In tests using simulated observations, the algorithm shows improved performance against two standard imaging approaches for extended sources, Multiscale-CLEAN and the Maximum Entropy Method.

Watching proteins function with 150-ps time-resolved X-ray crystallography

NASA Astrophysics Data System (ADS)

Anfinrud, Philip

2007-03-01

We have used time-resolved Laue crystallography to characterize ligand migration pathways and dynamics in wild-type and several mutant forms of myoglobin (Mb), a ligand-binding heme protein found in muscle tissue. In these pump-probe experiments, which were conducted on the ID09B time-resolved beamline at the European Synchrotron and Radiation Facility, a laser pulse photodissociates CO from an MbCO crystal and a suitably delayed X-ray pulse probes its structure via Laue diffraction. Single-site mutations in the vicinity of the heme pocket docking site were found to have a dramatic effect on ligand migration. To visualize this process, time-resolved electron density maps were stitched together into movies that unveil with <2-å spatial resolution and 150-ps time-resolution the correlated protein motions that accompany and/or mediate ligand migration. These studies help to illustrate at an atomic level relationships between protein structure, dynamics, and function.

DNA Meter: Energy Tunable, Quantitative Hybridization Assay

PubMed Central

Braunlin, William; Völker, Jens; Plum, G. Eric; Breslauer, Kenneth J.

2015-01-01

We describe a novel hybridization assay that employs a unique class of energy tunable, bulge loop-containing competitor strands (C*) that hybridize to a probe strand (P). Such initial “pre-binding” of a probe strand modulates its effective “availability” for hybridizing to a target site (T). More generally, the assay described here is based on competitive binding equilibria for a common probe strand (P) between such tunable competitor strands (C*) and a target strand (T). We demonstrate that loop variable, energy tunable families of C*P complexes exhibit enhanced discrimination between targets and mismatched targets, thereby reducing false positives/negatives. We refer to a C*P complex between a C* competitor single strand and the probe strand as a “tuning fork,” since the C* strand exhibits branch points (forks) at the duplex-bulge interfaces within the complex. By varying the loop to create families of such “tuning forks,” one can construct C*P “energy ladders” capable of resolving small differences within the target that may be of biological/functional consequence. The methodology further allows quantification of target strand concentrations, a determination heretofore not readily available by conventional hybridization assays. The dual ability of this tunable assay to discriminate and quantitate targets provides the basis for developing a technology we refer to as a “DNA Meter.” Here we present data that establish proof-of-principle for an in solution version of such a DNA Meter. We envision future applications of this tunable assay that incorporate surface bound/spatially resolved DNA arrays to yield enhanced discrimination and sensitivity. PMID:23529692

Spatially-resolved isotopic study of carbon trapped in ∼3.43 Ga Strelley Pool Formation stromatolites

NASA Astrophysics Data System (ADS)

Flannery, David T.; Allwood, Abigail C.; Summons, Roger E.; Williford, Kenneth H.; Abbey, William; Matys, Emily D.; Ferralis, Nicola

2018-02-01

The large isotopic fractionation of carbon associated with enzymatic carbon assimilation allows evidence for life's antiquity, and potentially the early operation of several extant metabolic pathways, to be derived from the stable carbon isotope record of sedimentary rocks. Earth's organic carbon isotope record extends to the Late Eoarchean-Early Paleoarchean: the age of the oldest known sedimentary rocks. However, complementary inorganic carbon reservoirs are poorly represented in the oldest units, and commonly reported bulk organic carbon isotope measurements do not capture the micro-scale isotopic heterogeneities that are increasingly reported from younger rocks. Here, we investigated the isotopic composition of the oldest paired occurrences of sedimentary carbonate and organic matter, which are preserved as dolomite and kerogen within textural biosignatures of the ∼3.43 Ga Strelley Pool Formation. We targeted least-altered carbonate phases in situ using microsampling techniques guided by non-destructive elemental mapping. Organic carbon isotope values were measured by spatially-resolved bulk analyses, and in situ using secondary ion mass spectrometry to target microscale domains of organic material trapped within inorganic carbon matrixes. Total observed fractionation of 13C ranges from -29 to -45‰. Our data are consistent with studies of younger Archean rocks that host biogenic stromatolites and organic-inorganic carbon pairs showing greater fractionation than expected for Rubisco fixation alone. We conclude that organic matter was fixed and/or remobilized by at least one metabolism in addition to the CBB cycle, possibly by the Wood-Ljungdahl pathway or methanogenesis-methanotrophy, in a shallow-water marine environment during the Paleoarchean.

Characterizing optical properties and spatial heterogeneity of human ovarian tissue using spatial frequency domain imaging

NASA Astrophysics Data System (ADS)

Nandy, Sreyankar; Mostafa, Atahar; Kumavor, Patrick D.; Sanders, Melinda; Brewer, Molly; Zhu, Quing

2016-10-01

A spatial frequency domain imaging (SFDI) system was developed for characterizing ex vivo human ovarian tissue using wide-field absorption and scattering properties and their spatial heterogeneities. Based on the observed differences between absorption and scattering images of different ovarian tissue groups, six parameters were quantitatively extracted. These are the mean absorption and scattering, spatial heterogeneities of both absorption and scattering maps measured by a standard deviation, and a fitting error of a Gaussian model fitted to normalized mean Radon transform of the absorption and scattering maps. A logistic regression model was used for classification of malignant and normal ovarian tissues. A sensitivity of 95%, specificity of 100%, and area under the curve of 0.98 were obtained using six parameters extracted from the SFDI images. The preliminary results demonstrate the diagnostic potential of the SFDI method for quantitative characterization of wide-field optical properties and the spatial distribution heterogeneity of human ovarian tissue. SFDI could be an extremely robust and valuable tool for evaluation of the ovary and detection of neoplastic changes of ovarian cancer.

Spatially-Resolved Influence of Temperature and Salinity on Stock and Recruitment Variability of Commercially Important Fishes in the North Sea

PubMed Central

Akimova, Anna; Núñez-Riboni, Ismael; Kempf, Alexander; Taylor, Marc H.

2016-01-01

Understanding of the processes affecting recruitment of commercially important fish species is one of the major challenges in fisheries science. Towards this aim, we investigated the relation between North Sea hydrography (temperature and salinity) and fish stock variables (recruitment, spawning stock biomass and pre-recruitment survival index) for 9 commercially important fishes using spatially-resolved cross-correlation analysis. We used high-resolution (0.2° × 0.2°) hydrographic data fields matching the maximal temporal extent of the fish population assessments (1948–2013). Our approach allowed for the identification of regions in the North Sea where environmental variables seem to be more influential on the fish stocks, as well as the regions of a lesser or nil influence. Our results confirmed previously demonstrated negative correlations between temperature and recruitment of cod and plaice and identified regions of the strongest correlations (German Bight for plaice and north-western North Sea for cod). We also revealed a positive correlation between herring spawning stock biomass and temperature in the Orkney-Shetland area, as well as a negative correlation between sole pre-recruitment survival index and temperature in the German Bight. A strong positive correlation between sprat stock variables and salinity in the central North Sea was also found. To our knowledge the results concerning correlations between North Sea hydrography and stocks’ dynamics of herring, sole and sprat are novel. The new information about spatial distribution of the correlation provides an additional help to identify mechanisms underlying these correlations. As an illustration of the utility of these results for fishery management, an example is provided that incorporates the identified environmental covariates in stock-recruitment models. PMID:27584155

Spatially resolved estimation of ozone-related mortality in the United States under two representative concentration pathways (RCPs) and their uncertainty

DOE PAGES

Kim, Young-Min; Zhou, Ying; Gao, Yang; ...

2014-11-16

We report that the spatial pattern of the uncertainty in air pollution-related health impacts due to climate change has rarely been studied due to the lack of high-resolution model simulations, especially under the Representative Concentration Pathways (RCPs), the latest greenhouse gas emission pathways. We estimated future tropospheric ozone (O 3) and related excess mortality and evaluated the associated uncertainties in the continental United States under RCPs. Based on dynamically downscaled climate model simulations, we calculated changes in O 3 level at 12 km resolution between the future (2057 and 2059) and base years (2001–2004) under a low-to-medium emission scenario (RCP4.5)more » and a fossil fuel intensive emission scenario (RCP8.5). We then estimated the excess mortality attributable to changes in O 3. Finally, we analyzed the sensitivity of the excess mortality estimates to the input variables and the uncertainty in the excess mortality estimation using Monte Carlo simulations. O 3-related premature deaths in the continental U.S. were estimated to be 1312 deaths/year under RCP8.5 (95 % confidence interval (CI): 427 to 2198) and ₋2118 deaths/year under RCP4.5 (95 % CI: ₋3021 to ₋1216), when allowing for climate change and emissions reduction. The uncertainty of O 3-related excess mortality estimates was mainly caused by RCP emissions pathways. Finally, excess mortality estimates attributable to the combined effect of climate and emission changes on O 3 as well as the associated uncertainties vary substantially in space and so do the most influential input variables. Spatially resolved data is crucial to develop effective community level mitigation and adaptation policy.« less

Spatially resolved estimation of ozone-related mortality in the United States under two representative concentration pathways (RCPs) and their uncertainty

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Young-Min; Zhou, Ying; Gao, Yang

We report that the spatial pattern of the uncertainty in air pollution-related health impacts due to climate change has rarely been studied due to the lack of high-resolution model simulations, especially under the Representative Concentration Pathways (RCPs), the latest greenhouse gas emission pathways. We estimated future tropospheric ozone (O 3) and related excess mortality and evaluated the associated uncertainties in the continental United States under RCPs. Based on dynamically downscaled climate model simulations, we calculated changes in O 3 level at 12 km resolution between the future (2057 and 2059) and base years (2001–2004) under a low-to-medium emission scenario (RCP4.5)more » and a fossil fuel intensive emission scenario (RCP8.5). We then estimated the excess mortality attributable to changes in O 3. Finally, we analyzed the sensitivity of the excess mortality estimates to the input variables and the uncertainty in the excess mortality estimation using Monte Carlo simulations. O 3-related premature deaths in the continental U.S. were estimated to be 1312 deaths/year under RCP8.5 (95 % confidence interval (CI): 427 to 2198) and ₋2118 deaths/year under RCP4.5 (95 % CI: ₋3021 to ₋1216), when allowing for climate change and emissions reduction. The uncertainty of O 3-related excess mortality estimates was mainly caused by RCP emissions pathways. Finally, excess mortality estimates attributable to the combined effect of climate and emission changes on O 3 as well as the associated uncertainties vary substantially in space and so do the most influential input variables. Spatially resolved data is crucial to develop effective community level mitigation and adaptation policy.« less

Quantitative analysis of biological tissues using Fourier transform-second-harmonic generation imaging

NASA Astrophysics Data System (ADS)

Ambekar Ramachandra Rao, Raghu; Mehta, Monal R.; Toussaint, Kimani C., Jr.

2010-02-01

We demonstrate the use of Fourier transform-second-harmonic generation (FT-SHG) imaging of collagen fibers as a means of performing quantitative analysis of obtained images of selected spatial regions in porcine trachea, ear, and cornea. Two quantitative markers, preferred orientation and maximum spatial frequency are proposed for differentiating structural information between various spatial regions of interest in the specimens. The ear shows consistent maximum spatial frequency and orientation as also observed in its real-space image. However, there are observable changes in the orientation and minimum feature size of fibers in the trachea indicating a more random organization. Finally, the analysis is applied to a 3D image stack of the cornea. It is shown that the standard deviation of the orientation is sensitive to the randomness in fiber orientation. Regions with variations in the maximum spatial frequency, but with relatively constant orientation, suggest that maximum spatial frequency is useful as an independent quantitative marker. We emphasize that FT-SHG is a simple, yet powerful, tool for extracting information from images that is not obvious in real space. This technique can be used as a quantitative biomarker to assess the structure of collagen fibers that may change due to damage from disease or physical injury.

Time-resolved hard x-ray spectrometer

NASA Astrophysics Data System (ADS)

Moy, Kenneth; Cuneo, Michael; McKenna, Ian; Keenan, Thomas; Sanford, Thomas; Mock, Ray

2006-08-01

Wired array studies are being conducted at the SNL Z accelerator to maximize the x-ray generation for inertial confinement fusion targets and high energy density physics experiments. An integral component of these studies is the characterization of the time-resolved spectral content of the x-rays. Due to potential spatial anisotropy in the emitted radiation, it is also critical to diagnose the time-evolved spectral content in a space-resolved manner. To accomplish these two measurement goals, we developed an x-ray spectrometer using a set of high-speed detectors (silicon PIN diodes) with a collimated field-of-view that converged on a 1-cm-diameter spot at the pinch axis. Spectral discrimination is achieved by placing high Z absorbers in front of these detectors. We built two spectrometers to permit simultaneous different angular views of the emitted radiation. Spectral data have been acquired from recent Z shots for the radial and axial (polar) views. UNSPEC 1 has been adapted to analyze and unfold the measured data to reconstruct the x-ray spectrum. The unfold operator code, UFO2, is being adapted for a more comprehensive spectral unfolding treatment.

Time-resolved structural studies at synchrotrons and X-ray free electron lasers: opportunities and challenges

PubMed Central

Neutze, Richard; Moffat, Keith

2012-01-01

X-ray free electron lasers (XFELs) are potentially revolutionary X-ray sources because of their very short pulse duration, extreme peak brilliance and high spatial coherence, features that distinguish them from today’s synchrotron sources. We review recent time-resolved Laue diffraction and time-resolved wide angle X-ray scattering (WAXS) studies at synchrotron sources, and initial static studies at XFELs. XFELs have the potential to transform the field of time-resolved structural biology, yet many challenges arise in devising and adapting hardware, experimental design and data analysis strategies to exploit their unusual properties. Despite these challenges, we are confident that XFEL sources are poised to shed new light on ultrafast protein reaction dynamics. PMID:23021004

The emergence of spatial cyberinfrastructure.

PubMed

Wright, Dawn J; Wang, Shaowen

2011-04-05

Cyberinfrastructure integrates advanced computer, information, and communication technologies to empower computation-based and data-driven scientific practice and improve the synthesis and analysis of scientific data in a collaborative and shared fashion. As such, it now represents a paradigm shift in scientific research that has facilitated easy access to computational utilities and streamlined collaboration across distance and disciplines, thereby enabling scientific breakthroughs to be reached more quickly and efficiently. Spatial cyberinfrastructure seeks to resolve longstanding complex problems of handling and analyzing massive and heterogeneous spatial datasets as well as the necessity and benefits of sharing spatial data flexibly and securely. This article provides an overview and potential future directions of spatial cyberinfrastructure. The remaining four articles of the special feature are introduced and situated in the context of providing empirical examples of how spatial cyberinfrastructure is extending and enhancing scientific practice for improved synthesis and analysis of both physical and social science data. The primary focus of the articles is spatial analyses using distributed and high-performance computing, sensor networks, and other advanced information technology capabilities to transform massive spatial datasets into insights and knowledge.

The emergence of spatial cyberinfrastructure

PubMed Central

Wright, Dawn J.; Wang, Shaowen

2011-01-01

Cyberinfrastructure integrates advanced computer, information, and communication technologies to empower computation-based and data-driven scientific practice and improve the synthesis and analysis of scientific data in a collaborative and shared fashion. As such, it now represents a paradigm shift in scientific research that has facilitated easy access to computational utilities and streamlined collaboration across distance and disciplines, thereby enabling scientific breakthroughs to be reached more quickly and efficiently. Spatial cyberinfrastructure seeks to resolve longstanding complex problems of handling and analyzing massive and heterogeneous spatial datasets as well as the necessity and benefits of sharing spatial data flexibly and securely. This article provides an overview and potential future directions of spatial cyberinfrastructure. The remaining four articles of the special feature are introduced and situated in the context of providing empirical examples of how spatial cyberinfrastructure is extending and enhancing scientific practice for improved synthesis and analysis of both physical and social science data. The primary focus of the articles is spatial analyses using distributed and high-performance computing, sensor networks, and other advanced information technology capabilities to transform massive spatial datasets into insights and knowledge. PMID:21467227

Spatially Resolved Patchy Lyα Emission within the Central Kiloparsec of a Strongly Lensed Quasar Host Galaxy at z = 2.8

NASA Astrophysics Data System (ADS)

Bayliss, Matthew B.; Sharon, Keren; Acharyya, Ayan; Gladders, Michael D.; Rigby, Jane R.; Bian, Fuyan; Bordoloi, Rongmon; Runnoe, Jessie; Dahle, Hakon; Kewley, Lisa; Florian, Michael; Johnson, Traci; Paterno-Mahler, Rachel

2017-08-01

We report the detection of extended Lyα emission from the host galaxy of SDSS J2222+2745, a strongly lensed quasar at z = 2.8. Spectroscopic follow-up clearly reveals extended Lyα in emission between two images of the central active galactic nucleus (AGN). We reconstruct the lensed quasar host galaxy in the source plane by applying a strong lens model to HST imaging and resolve spatial scales as small as ˜200 pc. In the source plane, we recover the host galaxy morphology to within a few hundred parsecs of the central AGN and map the extended Lyα emission to its physical origin on one side of the host galaxy at radii ˜0.5-2 kpc from the central AGN. There are clear morphological differences between the Lyα and rest-frame ultraviolet stellar continuum emission from the quasar host galaxy. Furthermore, the relative velocity profiles of quasar Lyα, host galaxy Lyα, and metal lines in outflowing gas reveal differences in the absorbing material affecting the AGN and host galaxy. These data indicate the presence of patchy local intervening gas in front of the central quasar and its host galaxy. This interpretation is consistent with the central luminous quasar being obscured across a substantial fraction of its surrounding solid angle, resulting in strong anisotropy in the exposure of the host galaxy to ionizing radiation from the AGN. This work demonstrates the power of strong-lensing-assisted studies to probe spatial scales that are currently inaccessible by other means.

Spatially Resolved Patchy Ly α Emission within the Central Kiloparsec of a Strongly Lensed Quasar Host Galaxy at z = 2.8

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bayliss, Matthew B.; Bordoloi, Rongmon; Sharon, Keren

We report the detection of extended Ly α emission from the host galaxy of SDSS J2222+2745, a strongly lensed quasar at z = 2.8. Spectroscopic follow-up clearly reveals extended Ly α in emission between two images of the central active galactic nucleus (AGN). We reconstruct the lensed quasar host galaxy in the source plane by applying a strong lens model to HST imaging and resolve spatial scales as small as ∼200 pc. In the source plane, we recover the host galaxy morphology to within a few hundred parsecs of the central AGN and map the extended Ly α emission tomore » its physical origin on one side of the host galaxy at radii ∼0.5–2 kpc from the central AGN. There are clear morphological differences between the Ly α and rest-frame ultraviolet stellar continuum emission from the quasar host galaxy. Furthermore, the relative velocity profiles of quasar Ly α , host galaxy Ly α , and metal lines in outflowing gas reveal differences in the absorbing material affecting the AGN and host galaxy. These data indicate the presence of patchy local intervening gas in front of the central quasar and its host galaxy. This interpretation is consistent with the central luminous quasar being obscured across a substantial fraction of its surrounding solid angle, resulting in strong anisotropy in the exposure of the host galaxy to ionizing radiation from the AGN. This work demonstrates the power of strong-lensing-assisted studies to probe spatial scales that are currently inaccessible by other means.« less

Super-resolved all-refocused image with a plenoptic camera

NASA Astrophysics Data System (ADS)

Wang, Xiang; Li, Lin; Hou, Guangqi

2015-12-01

This paper proposes an approach to produce the super-resolution all-refocused images with the plenoptic camera. The plenoptic camera can be produced by putting a micro-lens array between the lens and the sensor in a conventional camera. This kind of camera captures both the angular and spatial information of the scene in one single shot. A sequence of digital refocused images, which are refocused at different depth, can be produced after processing the 4D light field captured by the plenoptic camera. The number of the pixels in the refocused image is the same as that of the micro-lens in the micro-lens array. Limited number of the micro-lens will result in poor low resolution refocused images. Therefore, not enough details will exist in these images. Such lost details, which are often high frequency information, are important for the in-focus part in the refocused image. We decide to super-resolve these in-focus parts. The result of image segmentation method based on random walks, which works on the depth map produced from the 4D light field data, is used to separate the foreground and background in the refocused image. And focusing evaluation function is employed to determine which refocused image owns the clearest foreground part and which one owns the clearest background part. Subsequently, we employ single image super-resolution method based on sparse signal representation to process the focusing parts in these selected refocused images. Eventually, we can obtain the super-resolved all-focus image through merging the focusing background part and the focusing foreground part in the way of digital signal processing. And more spatial details will be kept in these output images. Our method will enhance the resolution of the refocused image, and just the refocused images owning the clearest foreground and background need to be super-resolved.

A simultaneous multi-slice selective J-resolved experiment for fully resolved scalar coupling information

NASA Astrophysics Data System (ADS)

Zeng, Qing; Lin, Liangjie; Chen, Jinyong; Lin, Yanqin; Barker, Peter B.; Chen, Zhong

2017-09-01

Proton-proton scalar coupling plays an important role in molecular structure elucidation. Many methods have been proposed for revealing scalar coupling networks involving chosen protons. However, determining all JHH values within a fully coupled network remains as a tedious process. Here, we propose a method termed as simultaneous multi-slice selective J-resolved spectroscopy (SMS-SEJRES) for simultaneously measuring JHH values out of all coupling networks in a sample within one experiment. In this work, gradient-encoded selective refocusing, PSYCHE decoupling and echo planar spectroscopic imaging (EPSI) detection module are adopted, resulting in different selective J-edited spectra extracted from different spatial positions. The proposed pulse sequence can facilitate the analysis of molecular structures. Therefore, it will interest scientists who would like to efficiently address the structural analysis of molecules.

In situ, spatially resolved biosignature detection at the microbial scale

NASA Astrophysics Data System (ADS)

Williford, K. H.; Eigenbrode, J. L.; Hallmann, C.; Kitajima, K.; Kozdon, R.; Summons, R. E.; Kudryavstev, A.; Lepot, K.; Schopf, J.; Spicuzza, M.; Sugitani, K.; Ushikubo, T.; van Kranendonk, M.; Valley, J. W.

2013-12-01

Whether life has ever existed beyond Earth is one of the great human questions. The Science Definition Team (SDT) for the proposed NASA Mars 2020 rover mission recently announced a suggested approach for NASA to 'demonstrate significant technical progress towards the future return of scientifically selected, well-documented samples to Earth' in part 'to investigate whether Mars was ever inhabited by microbial life.' The SDT further recommended a per-sample volume of 8 cm3 [1] (e.g., a core with a diameter of 1 cm and length of 10 cm). Such samples would be the first available for scientific inquiry with the potential to definitively answer the fundamental question of astrobiology, and their small volume would necessitate analysis with non- or minimally destructive techniques. Potential biosignatures include 'chemical, isotopic, mineralogical, and morphological features that can be created by life and also appear to be inconsistent with nonbiological processes'[1]. Guidelines for biosignature detection in extraterrestrial samples derive in part from the search for evidence of life in the most ancient sedimentary rocks on Earth, wherein the most compelling case for biogenicity is made when these 'chemical, isotopic, mineralogical, and morphological features' occur in association. Sedimentary rocks deposited on Earth prior to ~3.5 billion years ago (i.e., when persistent surface water [e.g., 2] likely supported habitable environments on Mars) have only very rarely escaped severe alteration by metamorphism and metasomatism. Understanding how these processes have operated on Earth through strategic interrogation of biosignature alteration records in (meta)sedimentary rocks is thus a critical task in the search for extraterrestrial life. Here we present techniques for and results of in situ, spatially resolved, non- or minimally destructive detection of morphological, elemental, molecular, and light stable isotopic biosignatures, as well as records of alteration, in

Geochemistry of Dissolved Organic Matter in a Spatially Highly Resolved Groundwater Petroleum Hydrocarbon Plume Cross-Section.

PubMed

Dvorski, Sabine E-M; Gonsior, Michael; Hertkorn, Norbert; Uhl, Jenny; Müller, Hubert; Griebler, Christian; Schmitt-Kopplin, Philippe

2016-06-07

At numerous groundwater sites worldwide, natural dissolved organic matter (DOM) is quantitatively complemented with petroleum hydrocarbons. To date, research has been focused almost exclusively on the contaminants, but detailed insights of the interaction of contaminant biodegradation, dominant redox processes, and interactions with natural DOM are missing. This study linked on-site high resolution spatial sampling of groundwater with high resolution molecular characterization of DOM and its relation to groundwater geochemistry across a petroleum hydrocarbon plume cross-section. Electrospray- and atmospheric pressure photoionization (ESI, APPI) ultrahigh resolution mass spectrometry (FT-ICR-MS) revealed a strong interaction between DOM and reactive sulfur species linked to microbial sulfate reduction, i.e., the key redox process involved in contaminant biodegradation. Excitation emission matrix (EEM) fluorescence spectroscopy in combination with Parallel Factor Analysis (PARAFAC) modeling attributed DOM samples to specific contamination traits. Nuclear magnetic resonance (NMR) spectroscopy evaluated the aromatic compounds and their degradation products in samples influenced by the petroleum contamination and its biodegradation. Our orthogonal high resolution analytical approach enabled a comprehensive molecular level understanding of the DOM with respect to in situ petroleum hydrocarbon biodegradation and microbial sulfate reduction. The role of natural DOM as potential cosubstrate and detoxification reactant may improve future bioremediation strategies.

Modeling spatially and spectrally resolved observations to diagnose the formation of elliptical galaxies

NASA Astrophysics Data System (ADS)

Snyder, Gregory Frantz

2013-03-01

In extragalactic astronomy, a central challenge is that we cannot directly watch what happens to galaxies before and after they are observed. This dissertation focuses on linking predictions of galaxy time-evolution directly with observations, evaluating how interactions, mergers, and other processes affect the appearance of elliptical galaxies. The primary approach is to combine hydrodynamical simulations of galaxy formation, including all major components, with dust radiative transfer to predict their observational signatures The current paradigm implies that a quiescent elliptical emerges following a formative starburst event. These trigger accretion onto the central supermassive black hole (SMBH), which then radiates as an active galactic nucleus (AGN). However, it is not clear the extent to which SMBH growth is fueled by these events nor how important is their energy input at setting the appearance of the remnant. This thesis presents results drawing from three phases in the formation of a typical elliptical: 1) I evaluate how to disentangle AGN from star formation signatures in mid-infrared spectra during a dust-enshrouded starburst, making testable predictions for robustly tracing SMBH growth with the James Webb Space Telescope; 2) I develop a model for the rate of merger-induced post-starburst galaxies selected from optical spectra, resolving tension between their observed rarity and merger rates from other estimates; and 3) I present results from Hubble Space Telescope imaging of elliptical galaxies in galaxy clusters at 1 < z < 2, the precursors of present-day massive clusters with M ~ 1015 solar masses, demonstrating that their stars formed over an extended period and ruling out the simplest model for their formation history. These results lend support to a stochastic formation history for ellipticals driven by mergers or interactions. However, significant uncertainties remain in how to evaluate the implications of galaxy appearance, in particular their

Discovery of Low-ionization Envelopes in the Planetary Nebula NGC 5189: Spatially-resolved Diagnostics from HST Observations

NASA Astrophysics Data System (ADS)

Danehkar, Ashkbiz; Karovska, Margarita; Maksym, Walter Peter; Montez, Rodolfo

2018-01-01

The planetary nebula NGC 5189 shows one of the most spectacular morphological structures among planetary nebulae with [WR]-type central stars. Using high-angular resolution HST/WFC3 imaging, we discovered inner, low-ionization structures within a region of 0.3 parsec × 0.2 parsec around the central binary system. We used Hα, [O III], and [S II] emission line images to construct line-ratio diagnostic maps, which allowed us to spatially resolve two distinct low-ionization envelopes within the inner, ionized gaseous environment, extending over a distance of 0.15 pc from the central binary. Both the low-ionization envelopes appear to be expanding along a NE to SW symmetric axis. The SW envelope appears smaller than its NE counterpart. Our diagnostic maps show that highly-ionized gas surrounds these low-ionization envelopes, which also include filamentary and clumpy structures. These envelopes could be a result of a powerful outburst from the central interacting binary, when one of the companions (now a [WR] star) was in its AGB evolutionary stage, with a strong mass-loss generating dense circumstellar shells. Dense material ejected from the progenitor AGB star is likely heated up as it propagates along a symmetric axis into the previously expelled low-density material. Our new diagnostic methodology is a powerful tool for high-angular resolution mapping of low-ionization structures in other planetary nebulae with complex structures possibly caused by past outbursts from their progenitors.

High spatial resolution time-resolved magnetic resonance angiography of lower extremity tumors at 3T

PubMed Central

Wu, Gang; Jin, Teng; Li, Ting; Morelli, John; Li, Xiaoming

2016-01-01

Abstract The aim of this study was to compare diagnostic value of high spatial resolution time-resolved magnetic resonance angiography with interleaved stochastic trajectory (TWIST) using Gadobutrol to Computed tomography angiography (CTA) for preoperative evaluation of lower extremity tumors. This prospective study was approved by the institutional review board. Fifty consecutive patients (31 men, 19 women, age range 18–80 years, average age 42.7 years) with lower extremity tumors underwent TWIST magnetic resonance angiography (MRA) and CTA. Digital subtraction angiography was available for 8 patients. Image quality of MRA was compared with CTA by 2 radiologists according to a 4-point Likert scale. Arterial involvement by tumor was compared using kappa test between MRA and CTA. The ability to identify feeding arteries and arterio-venous fistulae (AVF) was compared using Wilcoxon signed rank test and McNemar test, respectively. Image quality of MRA and CTA was rated without a statistically significant difference (3.88 ± 0.37 vs. 3.97 ± 0.16, P = 0.135). Intramodality agreement was high for the identification of arterial invasion (kappa = 0.806 ± 0.073 for Reader 1, kappa = 0.805 ± 0.073 for Reader 2). Readers found AVF in 27 of 50 MRA cases and 14 of 50 CTA cases (P < 0.001). Mean feeding arteries identified with MRA were significantly more than that with CTA (2.08 ± 1.72 vs. 1.62 ± 1.52, P = .02). TWIST MRA is a reliable imaging modality for the assessment of lower extremity tumors. TWIST MRA is comparable to CTA for the identification of AVF and feeding arteries. PMID:27631262

The SAMI Galaxy Survey: spatially resolving the environmental quenching of star formation in GAMA galaxies

NASA Astrophysics Data System (ADS)

Schaefer, A. L.; Croom, S. M.; Allen, J. T.; Brough, S.; Medling, A. M.; Ho, I.-T.; Scott, N.; Richards, S. N.; Pracy, M. B.; Gunawardhana, M. L. P.; Norberg, P.; Alpaslan, M.; Bauer, A. E.; Bekki, K.; Bland-Hawthorn, J.; Bloom, J. V.; Bryant, J. J.; Couch, W. J.; Driver, S. P.; Fogarty, L. M. R.; Foster, C.; Goldstein, G.; Green, A. W.; Hopkins, A. M.; Konstantopoulos, I. S.; Lawrence, J. S.; López-Sánchez, A. R.; Lorente, N. P. F.; Owers, M. S.; Sharp, R.; Sweet, S. M.; Taylor, E. N.; van de Sande, J.; Walcher, C. J.; Wong, O. I.

2017-01-01

We use data from the Sydney-AAO Multi-Object Integral Field Spectrograph Galaxy Survey and the Galaxy And Mass Assembly (GAMA) survey to investigate the spatially resolved signatures of the environmental quenching of star formation in galaxies. Using dust-corrected measurements of the distribution of Hα emission, we measure the radial profiles of star formation in a sample of 201 star-forming galaxies covering three orders of magnitude in stellar mass (M*; 108.1-1010.95 M⊙) and in fifth nearest neighbour local environment density (Σ5; 10-1.3-102.1 Mpc-2). We show that star formation rate gradients in galaxies are steeper in dense (log10(Σ5/Mpc2) > 0.5) environments by 0.58 ± 0.29 dex re^{-1} in galaxies with stellar masses in the range 10^{10} < M_{*}/M_{⊙} < 10^{11} and that this steepening is accompanied by a reduction in the integrated star formation rate. However, for any given stellar mass or environment density, the star formation morphology of galaxies shows large scatter. We also measure the degree to which the star formation is centrally concentrated using the unitless scale-radius ratio (r50,Hα/r50,cont), which compares the extent of ongoing star formation to previous star formation. With this metric, we find that the fraction of galaxies with centrally concentrated star formation increases with environment density, from ˜5 ± 4 per cent in low-density environments (log10(Σ5/Mpc2) < 0.0) to 30 ± 15 per cent in the highest density environments (log10(Σ5/Mpc2) > 1.0). These lines of evidence strongly suggest that with increasing local environment density, the star formation in galaxies is suppressed, and that this starts in their outskirts such that quenching occurs in an outside-in fashion in dense environments and is not instantaneous.

An inverse approach to determining spatially varying arterial compliance using ultrasound imaging

NASA Astrophysics Data System (ADS)

Mcgarry, Matthew; Li, Ronny; Apostolakis, Iason; Nauleau, Pierre; Konofagou, Elisa E.

2016-08-01

The mechanical properties of arteries are implicated in a wide variety of cardiovascular diseases, many of which are expected to involve a strong spatial variation in properties that can be depicted by diagnostic imaging. A pulse wave inverse problem (PWIP) is presented, which can produce spatially resolved estimates of vessel compliance from ultrasound measurements of the vessel wall displacements. The 1D equations governing pulse wave propagation in a flexible tube are parameterized by the spatially varying properties, discrete cosine transform components of the inlet pressure boundary conditions, viscous loss constant and a resistance outlet boundary condition. Gradient descent optimization is used to fit displacements from the model to the measured data by updating the model parameters. Inversion of simulated data showed that the PWIP can accurately recover the correct compliance distribution and inlet pressure under realistic conditions, even under high simulated measurement noise conditions. Silicone phantoms with known compliance contrast were imaged with a clinical ultrasound system. The PWIP produced spatially and quantitatively accurate maps of the phantom compliance compared to independent static property estimates, and the known locations of stiff inclusions (which were as small as 7 mm). The PWIP is necessary for these phantom experiments as the spatiotemporal resolution, measurement noise and compliance contrast does not allow accurate tracking of the pulse wave velocity using traditional approaches (e.g. 50% upstroke markers). Results from simulations indicate reflections generated from material interfaces may negatively affect wave velocity estimates, whereas these reflections are accounted for in the PWIP and do not cause problems.

Reporting of quantitative oxygen mapping in EPR imaging

NASA Astrophysics Data System (ADS)

Subramanian, Sankaran; Devasahayam, Nallathamby; McMillan, Alan; Matsumoto, Shingo; Munasinghe, Jeeva P.; Saito, Keita; Mitchell, James B.; Chandramouli, Gadisetti V. R.; Krishna, Murali C.

2012-01-01

Oxygen maps derived from electron paramagnetic resonance spectral-spatial imaging (EPRI) are based upon the relaxivity of molecular oxygen with paramagnetic spin probes. This technique can be combined with MRI to facilitate mapping of pO 2 values in specific anatomic locations with high precision. The co-registration procedure, which matches the physical and digital dimensions of EPR and MR images, may present the pO 2 map at the higher MRI resolution, exaggerating the spatial resolution of oxygen, making it difficult to precisely distinguish hypoxic regions from normoxic regions. The latter distinction is critical in monitoring the treatment of cancer by radiation and chemotherapy, since it is well-established that hypoxic regions are three or four times more resistant to treatment compared to normoxic regions. The aim of this article is to describe pO 2 maps based on the intrinsic resolution of EPRI. A spectral parameter that affects the intrinsic spatial resolution of EPRI is the full width at half maximum (FWHM) height of the gradient-free EPR absorption line in frequency-encoded imaging. In single point imaging too, the transverse relaxation times (T2∗) limit the resolution since the signal decays by exp(-tp/T2∗) where the delay time after excitation pulse, t p, is related to the resolution. Although the spin densities of two point objects may be resolved at this separation, it is inadequate to evaluate quantitative changes of pO 2 levels since the linewidths are proportionately affected by pO 2. A spatial separation of at least twice this resolution is necessary to correctly identify a change in pO 2 level. In addition, the pO 2 values are blurred by uncertainties arising from spectral dimensions. Blurring due to noise and low resolution modulates the pO 2 levels at the boundaries of hypoxic and normoxic regions resulting in higher apparent pO 2 levels in hypoxic regions. Therefore, specification of intrinsic resolution and pO 2 uncertainties are

Single-sensor system for spatially resolved, continuous, and multiparametric optical mapping of cardiac tissue

PubMed Central

Lee, Peter; Bollensdorff, Christian; Quinn, T. Alexander; Wuskell, Joseph P.; Loew, Leslie M.; Kohl, Peter

2011-01-01

Background Simultaneous optical mapping of multiple electrophysiologically relevant parameters in living myocardium is desirable for integrative exploration of mechanisms underlying heart rhythm generation under normal and pathophysiologic conditions. Current multiparametric methods are technically challenging, usually involving multiple sensors and moving parts, which contributes to high logistic and economic thresholds that prevent easy application of the technique. Objective The purpose of this study was to develop a simple, affordable, and effective method for spatially resolved, continuous, simultaneous, and multiparametric optical mapping of the heart, using a single camera. Methods We present a new method to simultaneously monitor multiple parameters using inexpensive off-the-shelf electronic components and no moving parts. The system comprises a single camera, commercially available optical filters, and light-emitting diodes (LEDs), integrated via microcontroller-based electronics for frame-accurate illumination of the tissue. For proof of principle, we illustrate measurement of four parameters, suitable for ratiometric mapping of membrane potential (di-4-ANBDQPQ) and intracellular free calcium (fura-2), in an isolated Langendorff-perfused rat heart during sinus rhythm and ectopy, induced by local electrical or mechanical stimulation. Results The pilot application demonstrates suitability of this imaging approach for heart rhythm research in the isolated heart. In addition, locally induced excitation, whether stimulated electrically or mechanically, gives rise to similar ventricular propagation patterns. Conclusion Combining an affordable camera with suitable optical filters and microprocessor-controlled LEDs, single-sensor multiparametric optical mapping can be practically implemented in a simple yet powerful configuration and applied to heart rhythm research. The moderate system complexity and component cost is destined to lower the threshold to broader

Quantitative Susceptibility Mapping of Human Brain Reflects Spatial Variation in Tissue Composition

PubMed Central

Li, Wei; Wu, Bing; Liu, Chunlei

2011-01-01

Image phase from gradient echo MRI provides a unique contrast that reflects brain tissue composition variations, such as iron and myelin distribution. Phase imaging is emerging as a powerful tool for the investigation of functional brain anatomy and disease diagnosis. However, the quantitative value of phase is compromised by its nonlocal and orientation dependent properties. There is an increasing need for reliable quantification of magnetic susceptibility, the intrinsic property of tissue. In this study, we developed a novel and accurate susceptibility mapping method that is also phase-wrap insensitive. The proposed susceptibility mapping method utilized two complementary equations: (1) the Fourier relationship of phase and magnetic susceptibility; and (2) the first-order partial derivative of the first equation in the spatial frequency domain. In numerical simulation, this method reconstructed the susceptibility map almost free of streaking artifact. Further, the iterative implementation of this method allowed for high quality reconstruction of susceptibility maps of human brain in vivo. The reconstructed susceptibility map provided excellent contrast of iron-rich deep nuclei and white matter bundles from surrounding tissues. Further, it also revealed anisotropic magnetic susceptibility in brain white matter. Hence, the proposed susceptibility mapping method may provide a powerful tool for the study of brain physiology and pathophysiology. Further elucidation of anisotropic magnetic susceptibility in vivo may allow us to gain more insight into the white matter microarchitectures. PMID:21224002

Automated Spatial Brain Normalization and Hindbrain White Matter Reference Tissue Give Improved [(18)F]-Florbetaben PET Quantitation in Alzheimer's Model Mice.

PubMed

Overhoff, Felix; Brendel, Matthias; Jaworska, Anna; Korzhova, Viktoria; Delker, Andreas; Probst, Federico; Focke, Carola; Gildehaus, Franz-Josef; Carlsen, Janette; Baumann, Karlheinz; Haass, Christian; Bartenstein, Peter; Herms, Jochen; Rominger, Axel

2016-01-01

Preclinical PET studies of β-amyloid (Aβ) accumulation are of growing importance, but comparisons between research sites require standardized and optimized methods for quantitation. Therefore, we aimed to evaluate systematically the (1) impact of an automated algorithm for spatial brain normalization, and (2) intensity scaling methods of different reference regions for Aβ-PET in a large dataset of transgenic mice. PS2APP mice in a 6 week longitudinal setting (N = 37) and another set of PS2APP mice at a histologically assessed narrow range of Aβ burden (N = 40) were investigated by [(18)F]-florbetaben PET. Manual spatial normalization by three readers at different training levels was performed prior to application of an automated brain spatial normalization and inter-reader agreement was assessed by Fleiss Kappa (κ). For this method the impact of templates at different pathology stages was investigated. Four different reference regions on brain uptake normalization were used to calculate frontal cortical standardized uptake value ratios (SUVRCTX∕REF), relative to raw SUVCTX. Results were compared on the basis of longitudinal stability (Cohen's d), and in reference to gold standard histopathological quantitation (Pearson's R). Application of an automated brain spatial normalization resulted in nearly perfect agreement (all κ≥0.99) between different readers, with constant or improved correlation with histology. Templates based on inappropriate pathology stage resulted in up to 2.9% systematic bias for SUVRCTX∕REF. All SUVRCTX∕REF methods performed better than SUVCTX both with regard to longitudinal stability (d≥1.21 vs. d = 0.23) and histological gold standard agreement (R≥0.66 vs. R≥0.31). Voxel-wise analysis suggested a physiologically implausible longitudinal decrease by global mean scaling. The hindbrain white matter reference (R mean = 0.75) was slightly superior to the brainstem (R mean = 0.74) and the cerebellum (R mean = 0.73). Automated

[Non-invasive, spatially resolved determination of tissue properties of the crystalline lens with regard to rheology, refractive index, density and protein concentration by using Brillouin spectroscopy].

PubMed

Reiss, S; Stachs, O; Guthoff, R; Stolz, H

2011-12-01

The confocal Brillouin spectroscopy is an innovative measurement method that allows the non-invasive determination of the rheological properties of materials. Its application in ophthalmology can offer the possibility to determine in-vivo the deformation properties of sections of transparent biological tissue such as the cornea or eye lens with spatial resolution. This seems to be a promising approach concerning current presbyopia research. Due to the spatially resolved detection of the viscoelastic lens properties, a better understanding of the natural aging process of the lens and the influences of different lens opacities on the stiffness is expected. From the obtained spectral data the relative protein levels, the relative refractive index profile and the relative density profile within the lens tissue can be derived in addition. A measurement set-up for confocal Brillouin microscopy based on spectral analysis of spontaneous Brillouin scattering signals by using a high-resolution dispersive device is presented. First in-vitro test results on animal and human lenses are presented and evaluated concerning their rheological significance. These data are compared with known research results. © Georg Thieme Verlag KG Stuttgart · New York.

Spatially resolved ultrasonic attenuation in resistance spot welds: implications for nondestructive testing.

PubMed

Mozurkewich, George; Ghaffari, Bita; Potter, Timothy J

2008-09-01

Spatial variation of ultrasonic attenuation and velocity has been measured in plane parallel specimens extracted from resistance spot welds. In a strong weld, attenuation is larger in the nugget than in the parent material, and the region of increased attenuation is surrounded by a ring of decreased attenuation. In the center of a stick weld, attenuation is even larger than in a strong weld, and the low-attenuation ring is absent. These spatial variations are interpreted in terms of differences in grain size and martensite formation. Measured frequency dependences indicate the presence of an additional attenuation mechanism besides grain scattering. The observed attenuations do not vary as commonly presumed with weld quality, suggesting that the common practice of using ultrasonic attenuation to indicate weld quality is not a reliable methodology.

Spatial reconstruction of single-cell gene expression data.

PubMed

Satija, Rahul; Farrell, Jeffrey A; Gennert, David; Schier, Alexander F; Regev, Aviv

2015-05-01

Spatial localization is a key determinant of cellular fate and behavior, but methods for spatially resolved, transcriptome-wide gene expression profiling across complex tissues are lacking. RNA staining methods assay only a small number of transcripts, whereas single-cell RNA-seq, which measures global gene expression, separates cells from their native spatial context. Here we present Seurat, a computational strategy to infer cellular localization by integrating single-cell RNA-seq data with in situ RNA patterns. We applied Seurat to spatially map 851 single cells from dissociated zebrafish (Danio rerio) embryos and generated a transcriptome-wide map of spatial patterning. We confirmed Seurat's accuracy using several experimental approaches, then used the strategy to identify a set of archetypal expression patterns and spatial markers. Seurat correctly localizes rare subpopulations, accurately mapping both spatially restricted and scattered groups. Seurat will be applicable to mapping cellular localization within complex patterned tissues in diverse systems.

The star formation history of the Hubble sequence: spatially resolved colour distributions of intermediate-redshift galaxies in the Hubble Deep Field

NASA Astrophysics Data System (ADS)

Abraham, R. G.; Ellis, R. S.; Fabian, A. C.; Tanvir, N. R.; Glazebrook, K.

1999-03-01

We analyse the spatially resolved colours of distant galaxies of known redshift in the Hubble Deep Field, using a new technique based on matching resolved four-band colour data to the predictions of evolutionary synthesis models. Given some simplifying assumptions, we demonstrate how our technique is capable of probing the evolutionary history of high-redshift systems, noting the specific advantage of observing galaxies at an epoch closer to the time of their formation. We quantify the relative age, dispersion in age, on-going star formation rate and star formation history of distinct components. We explicitly test for the presence of dust and quantify its effect on our conclusions. To demonstrate the potential of the method, we study the spirals and ellipticals in the near-complete sample of 32 I_814<21.9 mag galaxies with z~0.5 studied by Bouwens, Broadhurst & Silk. The dispersion of the internal colours of a sample of 0.4resolve the current controversy regarding the ages of galactic bulges. Dust and metallicity gradients are ruled out as major contributors to the colour dispersions that we observe in these systems. The median ages of bulge stars are found to be significantly higher than those in galactic discs, and they exhibit markedly different star formation histories. This result is inconsistent with a secular growth of bulges from disc instabilities, but is consistent with gradual disc formation by accretion of gas on to bulges, as predicted by

Influence of cutaneous and muscular circulation on spatially resolved versus standard Beer-Lambert near-infrared spectroscopy.

PubMed

Messere, Alessandro; Roatta, Silvestro

2013-12-01

The potential interference of cutaneous circulation on muscle blood volume and oxygenation monitoring by near-infrared spectroscopy (NIRS) remains an important limitation of this technique. Spatially resolved spectroscopy (SRS) was reported to minimize the contribution of superficial tissue layers in cerebral monitoring but this characteristic has never been documented in muscle tissue monitoring. This study aims to compare SRS with the standard Beer-Lambert (BL) technique in detecting blood volume changes selectively induced in muscle and skin. In 16 healthy subjects, the biceps brachii was investigated during isometric elbow flexion at 70% of the maximum voluntary contractions lasting 10 sec, performed before and after exposure of the upper arm to warm air flow. From probes applied over the muscle belly the following variables were recorded: total hemoglobin index (THI, SRS-based), total hemoglobin concentration (tHb, BL-based), tissue oxygenation index (TOI, SRS-based), and skin blood flow (SBF), using laser Doppler flowmetry. Blood volume indices exhibited similar changes during muscle contraction but only tHb significantly increased during warming (+5.2 ± 0.7 μmol/L·cm, an effect comparable to the increase occurring in postcontraction hyperemia), accompanying a 10-fold increase in SBF. Contraction-induced changes in tHb and THI were not substantially affected by warming, although the tHb tracing was shifted upward by (5.2 ± 3.5 μmol/L·cm, P < 0.01). TOI was not affected by cutaneous warming. In conclusion, SRS appears to effectively reject interference by SBF in both muscle blood volume and oxygenation monitoring. Instead, BL-based parameters should be interpreted with caution, whenever changes in cutaneous perfusion cannot be excluded.

Influence of cutaneous and muscular circulation on spatially resolved versus standard Beer–Lambert near‐infrared spectroscopy

PubMed Central

Messere, Alessandro; Roatta, Silvestro

2013-01-01

Abstract The potential interference of cutaneous circulation on muscle blood volume and oxygenation monitoring by near‐infrared spectroscopy (NIRS) remains an important limitation of this technique. Spatially resolved spectroscopy (SRS) was reported to minimize the contribution of superficial tissue layers in cerebral monitoring but this characteristic has never been documented in muscle tissue monitoring. This study aims to compare SRS with the standard Beer–Lambert (BL) technique in detecting blood volume changes selectively induced in muscle and skin. In 16 healthy subjects, the biceps brachii was investigated during isometric elbow flexion at 70% of the maximum voluntary contractions lasting 10 sec, performed before and after exposure of the upper arm to warm air flow. From probes applied over the muscle belly the following variables were recorded: total hemoglobin index (THI, SRS‐based), total hemoglobin concentration (tHb, BL‐based), tissue oxygenation index (TOI, SRS‐based), and skin blood flow (SBF), using laser Doppler flowmetry. Blood volume indices exhibited similar changes during muscle contraction but only tHb significantly increased during warming (+5.2 ± 0.7 μmol/L·cm, an effect comparable to the increase occurring in postcontraction hyperemia), accompanying a 10‐fold increase in SBF. Contraction‐induced changes in tHb and THI were not substantially affected by warming, although the tHb tracing was shifted upward by (5.2 ± 3.5 μmol/L·cm, P < 0.01). TOI was not affected by cutaneous warming. In conclusion, SRS appears to effectively reject interference by SBF in both muscle blood volume and oxygenation monitoring. Instead, BL‐based parameters should be interpreted with caution, whenever changes in cutaneous perfusion cannot be excluded. PMID:24744858

On Spatially Explicit Models of Epidemic and Endemic Cholera: The Haiti and Lake Kivu Case Studies.

NASA Astrophysics Data System (ADS)

Rinaldo, A.; Bertuzzo, E.; Mari, L.; Finger, F.; Casagrandi, R.; Gatto, M.; Rodriguez-Iturbe, I.

2014-12-01

The first part of the Lecture deals with the predictive ability of mechanistic models for the Haitian cholera epidemic. Predictive models of epidemic cholera need to resolve at suitable aggregation levels spatial data pertaining to local communities, epidemiological records, hydrologic drivers, waterways, patterns of human mobility and proxies of exposure rates. A formal model comparison framework provides a quantitative assessment of the explanatory and predictive abilities of various model settings with different spatial aggregation levels. Intensive computations and objective model comparisons show that parsimonious spatially explicit models accounting for spatial connections have superior explanatory power than spatially disconnected ones for short-to intermediate calibration windows. In general, spatially connected models show better predictive ability than disconnected ones. We suggest limits and validity of the various approaches and discuss the pathway towards the development of case-specific predictive tools in the context of emergency management. The second part deals with approaches suitable to describe patterns of endemic cholera. Cholera outbreaks have been reported in the Democratic Republic of the Congo since the 1970s. Here we employ a spatially explicit, inhomogeneous Markov chain model to describe cholera incidence in eight health zones on the shore of lake Kivu. Remotely sensed datasets of chlorophyll a concentration in the lake, precipitation and indices of global climate anomalies are used as environmental drivers in addition to baseline seasonality. The effect of human mobility is also modelled mechanistically. We test several models on a multi-year dataset of reported cholera cases. Fourteen models, accounting for different environmental drivers, are selected in calibration. Among these, the one accounting for seasonality, El Nino Southern Oscillation, precipitation and human mobility outperforms the others in cross-validation.

Fluorescence hyperspectral imaging (fHSI) using a spectrally resolved detector array

PubMed Central

Luthman, Anna Siri; Dumitru, Sebastian; Quiros‐Gonzalez, Isabel; Joseph, James

2017-01-01

Abstract The ability to resolve multiple fluorescent emissions from different biological targets in video rate applications, such as endoscopy and intraoperative imaging, has traditionally been limited by the use of filter‐based imaging systems. Hyperspectral imaging (HSI) facilitates the detection of both spatial and spectral information in a single data acquisition, however, instrumentation for HSI is typically complex, bulky and expensive. We sought to overcome these limitations using a novel robust and low cost HSI camera based on a spectrally resolved detector array (SRDA). We integrated this HSI camera into a wide‐field reflectance‐based imaging system operating in the near‐infrared range to assess the suitability for in vivo imaging of exogenous fluorescent contrast agents. Using this fluorescence HSI (fHSI) system, we were able to accurately resolve the presence and concentration of at least 7 fluorescent dyes in solution. We also demonstrate high spectral unmixing precision, signal linearity with dye concentration and at depth in tissue mimicking phantoms, and delineate 4 fluorescent dyes in vivo. Our approach, including statistical background removal, could be directly generalised to broader spectral ranges, for example, to resolve tissue reflectance or autofluorescence and in future be tailored to video rate applications requiring snapshot HSI data acquisition. PMID:28485130

Velocity Measurement in Carotid Artery: Quantitative Comparison of Time-Resolved 3D Phase-Contrast MRI and Image-based Computational Fluid Dynamics

PubMed Central

Sarrami-Foroushani, Ali; Nasr Esfahany, Mohsen; Nasiraei Moghaddam, Abbas; Saligheh Rad, Hamidreza; Firouznia, Kavous; Shakiba, Madjid; Ghanaati, Hossein; Wilkinson, Iain David; Frangi, Alejandro Federico

2015-01-01

Background: Understanding hemodynamic environment in vessels is important for realizing the mechanisms leading to vascular pathologies. Objectives: Three-dimensional velocity vector field in carotid bifurcation is visualized using TR 3D phase-contrast magnetic resonance imaging (TR 3D PC MRI) and computational fluid dynamics (CFD). This study aimed to present a qualitative and quantitative comparison of the velocity vector field obtained by each technique. Subjects and Methods: MR imaging was performed on a 30-year old male normal subject. TR 3D PC MRI was performed on a 3 T scanner to measure velocity in carotid bifurcation. 3D anatomical model for CFD was created using images obtained from time-of-flight MR angiography. Velocity vector field in carotid bifurcation was predicted using CFD and PC MRI techniques. A statistical analysis was performed to assess the agreement between the two methods. Results: Although the main flow patterns were the same for the both techniques, CFD showed a greater resolution in mapping the secondary and circulating flows. Overall root mean square (RMS) errors for all the corresponding data points in PC MRI and CFD were 14.27% in peak systole and 12.91% in end diastole relative to maximum velocity measured at each cardiac phase. Bland-Altman plots showed a very good agreement between the two techniques. However, this study was not aimed to validate any of methods, instead, the consistency was assessed to accentuate the similarities and differences between Time-resolved PC MRI and CFD. Conclusion: Both techniques provided quantitatively consistent results of in vivo velocity vector fields in right internal carotid artery (RCA). PC MRI represented a good estimation of main flow patterns inside the vasculature, which seems to be acceptable for clinical use. However, limitations of each technique should be considered while interpreting results. PMID:26793288

CMEIAS color segmentation: an improved computing technology to process color images for quantitative microbial ecology studies at single-cell resolution.

PubMed

Gross, Colin A; Reddy, Chandan K; Dazzo, Frank B

2010-02-01

Quantitative microscopy and digital image analysis are underutilized in microbial ecology largely because of the laborious task to segment foreground object pixels from background, especially in complex color micrographs of environmental samples. In this paper, we describe an improved computing technology developed to alleviate this limitation. The system's uniqueness is its ability to edit digital images accurately when presented with the difficult yet commonplace challenge of removing background pixels whose three-dimensional color space overlaps the range that defines foreground objects. Image segmentation is accomplished by utilizing algorithms that address color and spatial relationships of user-selected foreground object pixels. Performance of the color segmentation algorithm evaluated on 26 complex micrographs at single pixel resolution had an overall pixel classification accuracy of 99+%. Several applications illustrate how this improved computing technology can successfully resolve numerous challenges of complex color segmentation in order to produce images from which quantitative information can be accurately extracted, thereby gain new perspectives on the in situ ecology of microorganisms. Examples include improvements in the quantitative analysis of (1) microbial abundance and phylotype diversity of single cells classified by their discriminating color within heterogeneous communities, (2) cell viability, (3) spatial relationships and intensity of bacterial gene expression involved in cellular communication between individual cells within rhizoplane biofilms, and (4) biofilm ecophysiology based on ribotype-differentiated radioactive substrate utilization. The stand-alone executable file plus user manual and tutorial images for this color segmentation computing application are freely available at http://cme.msu.edu/cmeias/ . This improved computing technology opens new opportunities of imaging applications where discriminating colors really matter most

Selection, Development and Results for The RESOLVE Regolith Volatiles Characterization Analytical System

NASA Technical Reports Server (NTRS)

Lueck, Dale E.; Captain, Janine E.; Gibson, Tracy L.; Peterson, Barbara V.; Berger, Cristina M.; Levine, Lanfang

2008-01-01

The RESOLVE project requires an analytical system to identify and quantitate the volatiles released from a lunar drill core sample as it is crushed and heated to 150 C. The expected gases and their range of concentrations were used to assess Gas Chromatography (GC) and Mass Spectrometry (MS), along with specific analyzers for use on this potential lunar lander. The ability of these systems to accurately quantitate water and hydrogen in an unknown matrix led to the selection of a small MEMS commercial process GC for use in this project. The modification, development and testing of this instrument for the specific needs of the project is covered.

Spatially Resolved Sensitivity of Single-Particle Plasmon Sensors

PubMed Central

2018-01-01

The high sensitivity of localized surface plasmon resonance sensors to the local refractive index allows for the detection of single-molecule binding events. Though binding events of single objects can be detected by their induced plasmon shift, the broad distribution of observed shifts remains poorly understood. Here, we perform a single-particle study wherein single nanospheres bind to a gold nanorod, and relate the observed plasmon shift to the binding location using correlative microscopy. To achieve this we combine atomic force microscopy to determine the binding location, and single-particle spectroscopy to determine the corresponding plasmon shift. As expected, we find a larger plasmon shift for nanospheres binding at the tip of a rod compared to its sides, in good agreement with numerical calculations. However, we also find a broad distribution of shifts even for spheres that were bound at a similar location to the nanorod. Our correlative approach allows us to disentangle effects of nanoparticle dimensions and binding location, and by comparison to numerical calculations we find that the biggest contributor to this observed spread is the dispersion in nanosphere diameter. These experiments provide insight into the spatial sensitivity and signal-heterogeneity of single-particle plasmon sensors and provides a framework for signal interpretation in sensing applications. PMID:29520315

Resolving galaxy cluster gas properties at z ˜ 1 with XMM-Newton and Chandra

NASA Astrophysics Data System (ADS)

Bartalucci, I.; Arnaud, M.; Pratt, G. W.; Démoclès, J.; van der Burg, R. F. J.; Mazzotta, P.

2017-02-01

Massive, high-redshift, galaxy clusters are useful laboratories to test cosmological models and to probe structure formation and evolution, but observations are challenging due to cosmological dimming and angular distance effects. Here we present a pilot X-ray study of the five most massive (M500 > 5 × 1014M⊙), distant (z 1), clusters detected via the Sunyaev-Zel'Dovich effect. We optimally combine XMM-Newton and Chandra X-ray observations by leveraging the throughput of XMM-Newton to obtain spatially-resolved spectroscopy, and the spatial resolution of Chandra to probe the bright inner parts and to detect embedded point sources. Capitalising on the excellent agreement in flux-related measurements, we present a new method to derive the density profiles, which are constrained in the centre by Chandra and in the outskirts by XMM-Newton. We show that the Chandra-XMM-Newton combination is fundamental for morphological analysis at these redshifts, the Chandra resolution being required to remove point source contamination, and the XMM-Newton sensitivity allowing higher significance detection of faint substructures. Measuring the morphology using images from both instruments, we found that the sample is dominated by dynamically disturbed objects. We use the combined Chandra-XMM-Newton density profiles and spatially-resolved temperature profiles to investigate thermodynamic quantities including entropy and pressure. From comparison of the scaled profiles with the local REXCESS sample, we find no significant departure from standard self-similar evolution, within the dispersion, at any radius, except for the entropy beyond 0.7 R500. The baryon mass fraction tends towards the cosmic value, with a weaker dependence on mass than that observed in the local Universe. We make a comparison with the predictions from numerical simulations. The present pilot study demonstrates the utility and feasibility of spatially-resolved analysis of individual objects at high-redshift through

Time-resolved imaging refractometry of microbicidal films using quantitative phase microscopy.

PubMed

Rinehart, Matthew T; Drake, Tyler K; Robles, Francisco E; Rohan, Lisa C; Katz, David; Wax, Adam

2011-12-01

Quantitative phase microscopy is applied to image temporal changes in the refractive index (RI) distributions of solutions created by microbicidal films undergoing hydration. We present a novel method of using an engineered polydimethylsiloxane structure as a static phase reference to facilitate calibration of the absolute RI across the entire field. We present a study of dynamic structural changes in microbicidal films during hydration and subsequent dissolution. With assumptions about the smoothness of the phase changes induced by these films, we calculate absolute changes in the percentage of film in regions across the field of view.

Development of a New Time-Resolved Laser-Induced Fluorescence Technique

NASA Astrophysics Data System (ADS)

Durot, Christopher; Gallimore, Alec

2012-10-01

We are developing a time-resolved laser-induced fluorescence (LIF) technique to interrogate the ion velocity distribution function (VDF) of EP thruster plumes down to the microsecond time scale. Better measurements of dynamic plasma processes will lead to improvements in simulation and prediction of thruster operation and erosion. We present the development of the new technique and results of initial tests. Signal-to-noise ratio (SNR) is often a challenge for LIF studies, and it is only more challenging for time-resolved measurements since a lock-in amplifier cannot be used with a long time constant. The new system uses laser modulation on the order of MHz, which enables the use of electronic filtering and phase-sensitive detection to improve SNR while preserving time-resolved information. Statistical averaging over many cycles to further improve SNR is done in the frequency domain. This technique can have significant advantages, including (1) larger spatial maps enabled by shorter data acquisition time and (2) the ability to average data without creating a phase reference by modifying the thruster operating condition with a periodic cutoff in discharge current, which can modify the ion velocity distribution.

High spatial resolution compressed sensing (HSPARSE) functional MRI.

PubMed

Fang, Zhongnan; Van Le, Nguyen; Choy, ManKin; Lee, Jin Hyung

2016-08-01

To propose a novel compressed sensing (CS) high spatial resolution functional MRI (fMRI) method and demonstrate the advantages and limitations of using CS for high spatial resolution fMRI. A randomly undersampled variable density spiral trajectory enabling an acceleration factor of 5.3 was designed with a balanced steady state free precession sequence to achieve high spatial resolution data acquisition. A modified k-t SPARSE method was then implemented and applied with a strategy to optimize regularization parameters for consistent, high quality CS reconstruction. The proposed method improves spatial resolution by six-fold with 12 to 47% contrast-to-noise ratio (CNR), 33 to 117% F-value improvement and maintains the same temporal resolution. It also achieves high sensitivity of 69 to 99% compared the original ground-truth, small false positive rate of less than 0.05 and low hemodynamic response function distortion across a wide range of CNRs. The proposed method is robust to physiological noise and enables detection of layer-specific activities in vivo, which cannot be resolved using the highest spatial resolution Nyquist acquisition. The proposed method enables high spatial resolution fMRI that can resolve layer-specific brain activity and demonstrates the significant improvement that CS can bring to high spatial resolution fMRI. Magn Reson Med 76:440-455, 2016. © 2015 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. © 2015 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine.

Nanometer-resolved chemical analyses of femtosecond laser-induced periodic surface structures on titanium

NASA Astrophysics Data System (ADS)

Kirner, Sabrina V.; Wirth, Thomas; Sturm, Heinz; Krüger, Jörg; Bonse, Jörn

2017-09-01

The chemical characteristics of two different types of laser-induced periodic surface structures (LIPSS), so-called high and low spatial frequency LIPSS (HSFL and LSFL), formed upon irradiation of titanium surfaces by multiple femtosecond laser pulses in air (30 fs, 790 nm, 1 kHz), are analyzed by various optical and electron beam based surface analytical techniques, including micro-Raman spectroscopy, energy dispersive X-ray analysis, X-ray photoelectron spectroscopy, and Auger electron spectroscopy. The latter method was employed in a high-resolution mode being capable of spatially resolving even the smallest HSFL structures featuring spatial periods below 100 nm. In combination with an ion sputtering technique, depths-resolved chemical information of superficial oxidation processes was obtained, revealing characteristic differences between the two different types of LIPSS. Our results indicate that a few tens of nanometer shallow HSFL are formed on top of a ˜150 nm thick graded superficial oxide layer without sharp interfaces, consisting of amorphous TiO2 and partially crystallized Ti2O3. The larger LSFL structures with periods close to the irradiation wavelength originate from the laser-interaction with metallic titanium. They are covered by a ˜200 nm thick amorphous oxide layer, which consists mainly of TiO2 (at the surface) and other titanium oxide species of lower oxidation states underneath.

Energy-resolved CT imaging with a photon-counting silicon-strip detector

NASA Astrophysics Data System (ADS)

Persson, Mats; Huber, Ben; Karlsson, Staffan; Liu, Xuejin; Chen, Han; Xu, Cheng; Yveborg, Moa; Bornefalk, Hans; Danielsson, Mats

2014-03-01

Photon-counting detectors are promising candidates for use in the next generation of x-ray CT scanners. Among the foreseen benefits are higher spatial resolution, better trade-off between noise and dose, and energy discriminating capabilities. Silicon is an attractive detector material because of its low cost, mature manufacturing process and high hole mobility. However, it is sometimes claimed to be unsuitable for use in computed tomography because of its low absorption efficiency and high fraction of Compton scatter. The purpose of this work is to demonstrate that high-quality energy-resolved CT images can nonetheless be acquired with clinically realistic exposure parameters using a photon-counting silicon-strip detector with eight energy thresholds developed in our group. We use a single detector module, consisting of a linear array of 50 0.5 × 0.4 mm detector elements, to image a phantom in a table-top lab setup. The phantom consists of a plastic cylinder with circular inserts containing water, fat and aqueous solutions of calcium, iodine and gadolinium, in different concentrations. We use basis material decomposition to obtain water, calcium, iodine and gadolinium basis images and demonstrate that these basis images can be used to separate the different materials in the inserts. We also show results showing that the detector has potential for quantitative measurements of substance concentrations.