Science.gov

Sample records for quimioterapia es independiente

  1. Complicaciones orales de la quimioterapia y la radioterapia (PDQ®)—Versión para pacientes

    Cancer.gov

    Resumen de información revisada por expertos acerca de las complicaciones orales, como la mucositis y la disfunción de la glándula salival, que se presentan en pacientes de cáncer tratados con quimioterapia y radioterapia dirigida a la cabeza y el cuello.

  2. Neuropatía periférica inducida por quimioterapia

    Cancer.gov

    Artículo sobre un efecto secundario de la quimioterapia que causa dolor y malestar en las manos y los pies. También incluye información sobre los esfuerzos para mejorar las opciones de detección, tratamiento y prevención.

  3. [Analysis of the continuity, circulation and productivity of the Revista Española de Quimioterapia].

    PubMed

    Gimeno Sieres, E

    2007-06-01

    The primary objective of this study was to compare some of the bibliometric indicators of the continuity, circulation and productivity of the Revista Espanola de Quimioterapia up to 2003 with other spanish journals of Pharmacy and Pharmacology. This was done by reviewing periodicals directories, such as the ISSN (International Standard Serial Number/Número Internacional Normalizado de Publicaciones Seriadas) and ULRICH'S (Periodicals Directory), as well as the CDU (Classification Universal Decimal), national and international databases including IME (Indice Médico Español), ICYT (Indice Espanol de Ciencia y Tecnologia), IPA (International Pharmaceutical Abstracts), SCI Expanded (Science Citation Index Expanded), MEDLINE (Index Medicus), EMBASE (Excerpta Medica), BIOSIS PREVIEWS, ANALYTICAL ABSTRACTS, FSTA (Food Science and Technology Abstracts), SCIFINDER SCHOLAR and CHEMISTRY CITATION INDEX. According to the results, the Revista Española de Quimioterapia, in publication for 15 years, is widely distributed and has a good rating among other scientific journals of the same discipline. PMID:17893754

  4. [Analysis of the continuity, circulation and productivity of the Revista Española de Quimioterapia].

    PubMed

    Gimeno Sieres, E

    2007-06-01

    The primary objective of this study was to compare some of the bibliometric indicators of the continuity, circulation and productivity of the Revista Espanola de Quimioterapia up to 2003 with other spanish journals of Pharmacy and Pharmacology. This was done by reviewing periodicals directories, such as the ISSN (International Standard Serial Number/Número Internacional Normalizado de Publicaciones Seriadas) and ULRICH'S (Periodicals Directory), as well as the CDU (Classification Universal Decimal), national and international databases including IME (Indice Médico Español), ICYT (Indice Espanol de Ciencia y Tecnologia), IPA (International Pharmaceutical Abstracts), SCI Expanded (Science Citation Index Expanded), MEDLINE (Index Medicus), EMBASE (Excerpta Medica), BIOSIS PREVIEWS, ANALYTICAL ABSTRACTS, FSTA (Food Science and Technology Abstracts), SCIFINDER SCHOLAR and CHEMISTRY CITATION INDEX. According to the results, the Revista Española de Quimioterapia, in publication for 15 years, is widely distributed and has a good rating among other scientific journals of the same discipline.

  5. Complicaciones orales de la quimioterapia y la radioterapia (PDQ®)—Versión para profesionales de salud

    Cancer.gov

    Resumen de información revisada por expertos acerca de las complicaciones orales, como la mucositis y la disfunción de la glándula salival, que se presentan en pacientes de cáncer tratados con quimioterapia y radioterapia a la cabeza y el cuello.

  6. Combinación de radioterapia con quimioterapia mejora la supervivencia con raro cáncer cerebral

    Cancer.gov

    Los resultados de dos estudios clínicos de seguimiento a largo plazo confirman que ciertos pacientes viven substancialmente más si se les trata con una combinación de quimioterapia y radioterapia en comparación con radioterapia solamente.

  7. Añadir quimioterapia después de la radioterapia mejora la supervivencia de adultos con un tipo de tu

    Cancer.gov

    Adultos con gliomas de grado bajo, una forma de tumor cerebral, que recibieron tratamiento con quimioterapia después de la radioterapia vivieron más tiempo que pacientes que recibieron solo radioterapia, según los resultados de seguimiento a largo plazo d

  8. ES Review, Fall 2006

    ERIC Educational Resources Information Center

    Education Sector, 2006

    2006-01-01

    "ES Review" brings together in one publication recent Education Sector work that reflects both the reach of its policy projects and its commitment to translating the complexities of education policymaking for a wide range of audiences. Articles in this first edition of "ES Review" are: (1) Community College Confidential (Robert Margolis); (2)…

  9. ES H action plan

    SciTech Connect

    Not Available

    1991-01-01

    This document contains planned actions to correct the deficiencies identified in the Pre-Tiger Team Self-Assessment (PTTSA), January 1991, of Sandia National Laboratories (SNL -- Albuquerque, New Mexico; Tonopah, Nevada; and Kauai, Hawaii). The Self-Assessment was conducted by a Self-Assessment Working Group consisting of 19 department managers, with support from Environment, Safety, and Health (ES H) professionals, from October through December 1990. Findings from other past audits, dating back to 1985, were reviewed and compared with the PTTSA findings to determine if additional findings, key findings, or root causes were warranted. The resulting ES H Action Plan and individual planned actions were prepared by the ES H Action Plan Project Group with assistance from the Program owners/authors during February and March 1991. The plan was reviewed by SNL Management in April 1991. This document serves as a planning instrument for the Laboratories to aid in the scoping and sizing of activities related to ES H compliance for the coming five years. It will be modified as required to ensure a workload/funding balance and to address the findings resulting from the Tiger Team assessment at SNL, Albuquerque. The process of producing this document has served well to prepare SNL, Albuquerque, for the coming task of producing the required post-Tiger Team action plan document. 8 tabs.

  10. Determining the half-lives of /sup 253/Es, /sup 254/Es, /sup 254m/Es, /sup 255/Es, /sup 257/Es, /sup 256/Fm

    SciTech Connect

    Popov, Yu.S.; Timofeev, G.A.; Mishenev, V.B.; Kovantsev, V.N.; Elesin, A.A.

    1988-03-01

    Semiconductor alpha, gamma, and x-ray spectrometry has been used to identify einsteinium and fermium isotopes having mass numbers 253-257 in californium targets irradiated in the central channel of the high-flux SM-2 reactor. Half-life measurements have been made for /sup 253/Es, /sup 254/Es, /sup 254m/Es, /sup 255/Es, /sup 257/Es, /sup 256/Fm. The measurements are compared with published data.

  11. ES '70 REPORT NUMBER 2.

    ERIC Educational Resources Information Center

    1967

    IN A JULY 1967 MEETING, THE EDUCATIONAL SYSTEM FOR THE SEVENTIES (ES '70) EXECUTIVE COMMITTEE (1) REVIEWED THE PLANS FOR THE SUMMER WORKSHOP FOR LOCAL ES '70 PROGRAM COORDINATORS TO BE HELD IN AUGUST 1967, (2) REVIEWED THE LIST OF 15 PARTICIPATING SCHOOL SYSTEMS--BLOOMFIELD HILLS, MICHIGAN, MINEOLA, NEW YORK, SAN MATEO, CALIFORNIA, MONROE,…

  12. ES Review: Selections from 2007

    ERIC Educational Resources Information Center

    Smiles, Robin, Ed.

    2008-01-01

    The second edition of the "ES Review" brings together, in one setting, abridged versions of research reports, outside articles and op-eds, book reviews, and other Education Sector publications. The 2007 edition features: (1) K-12 Accountability (Laboratories of Reform: Virtual High Schools and Innovation in Public Education (Bill Tucker); Labeled:…

  13. ES '70 NEWS. VOLUME 1, NO. 2.

    ERIC Educational Resources Information Center

    Shelly (E.F.) and Co., New York, NY.

    SPECIFICS OF IMPLEMENTING THE OBJECTIVES OF THE EDUCATIONAL SYSTEM FOR THE SEVENTIES (ES '70) PROGRAM TO RELATE EDUCATION TO THE NEEDS OF AMERICAN YOUTH, CLARIFYING THE STRUCTURE OF THE ES '70 NETWORK, AND CHARTING THE BROAD DIRECTION OF THE LONG-RANGE ACTIVITIES OF THE PARTICIPANTS WERE THE PRIMARY SUBJECTS FOR REVIEW AT THE SECOND ANNUAL MEETING…

  14. Identification, characterization, and chromosomal localization of the human homolog (hES) of ES/130

    SciTech Connect

    Basson, C.T.; Morton, C.C.; MacRae, C.A.

    1996-08-01

    The chicken extracellular matrix glycoprotein ES/130 is necessary for epithelial-mesenchymal transformation in the developing hear and is also expressed in noncardiac chicken tissues such as limb and notochord. We have identified hES, the human homology of chicken ES/130. Fluorescence in situ hybridization analysis (FISH) localizes hES to human chromosome 20p11.2-p12. FISH analyses of individuals with 20p12 deletions and affected by Alagille syndrome exclude hES as a candidate gene for this disorder. Reverse transcriptase-polymerase chain reaction studies reveal that hES is expressed in both fetal and adult human tissues and that hES expression in the left ventricle is increased in the failing adult heart. Further studies will evaluate how hES mutations may relate to congenital human cardiac and skeletal anomalies as well as cardiac remodeling in the adult. 16 refs., 2 figs.

  15. Fission barriers for /sup 255/Es, /sup 256/Es, and /sup 255/Fm

    SciTech Connect

    Britt, H.C.; Cheifetz, E.; Hoffman, D.C.; Wilhelmy, J.B.; Dupzyk, R.J.; Lougheed, R.W.

    1980-02-01

    Fission coincidence data are presented for (d,pf), (t,pf), and (/sup 3/He,df) reactions on a /sup 254/Es target. A possible resonance is observed in /sup 255/Es. Estimates for the height of the first peak of the fission barrier for /sup 255/Es, /sup 256/Es, and /sup 255/Fm are presented. The possibility of additional structure in the potential energy surface in the vicinity of the first peak of the fission barrier is discussed.

  16. Sporadic Layer es and Siesmic Activity

    NASA Astrophysics Data System (ADS)

    Alimov, Obid; Blokhin, Alexandr; Kalashnikova, Tatyana

    2016-07-01

    To determine the influence of seismogenic disturbances on the calm state of the iono-sphere and assess the impact of turbulence development in sporadic-E during earthquake prepa-ration period we calculated the variation in the range of semitransparency ∆fES = f0ES - fbES. The study was based primarily on the ionograms obtained by vertical sounding of the ionosphere at Dushanbe at nighttime station from 15 to 29 August 1986. In this time period four successive earthquakes took place, which serves the purpose of this study of the impact of seis-mogenic processes on the intensity of the continuous generation of ionospheric turbulence. Analysis of the results obtained for seismic-ionospheric effects of 1986 earthquakes at station Dushanbe has shown that disturbance of ionospheric parameters during earthquake prepa-ration period displays a pronounced maximum with a duration of t = 1-6 hours. Ionospheric effects associated with the processes of earthquake preparation emerge quite predictably, which verifies seismogenic disturbances in the ionosphere. During the preparation of strong earthquakes, ionograms of vertical sounding produced at station Dushanbe - near the epicenter area - often shown the phenomenon of spreading traces of sporadic Es. It is assumed that the duration of manifestation of seismic ionospheric precursors in Du-shanbe τ = 1 - 6 hours may be associated with deformation processes in the Earth's crust and var-ious faults, as well as dissimilar properties of the environment of the epicentral area. It has been shown that for earthquakes with 4.5 ≤ M ≤ 5.5 1-2 days prior to the event iono-spheric perturbations in the parameters of the sporadic layer Es and an increase in the value of the range of semitransparency Es - ΔfEs were observed, which could lead to turbulence at altitudes of 100-130 km.

  17. ES Review: Selections from 2008 & 2009

    ERIC Educational Resources Information Center

    Smiles, Robin, Ed.

    2009-01-01

    This third edition of the "ES Review" brings together, in one setting, some of the best work from 2008-09. It features: (1) K-12 Accountability (Measuring Skills for the 21st Century (Elena Silva); Beyond the Bubble: Technology and the Future of Student Assessment (Bill Tucker); Testing the Limits (Bill Tucker); Changing the Game: The Federal Role…

  18. ES Review: Selections from 2009 and 2010

    ERIC Educational Resources Information Center

    Smiles, Robin, Ed.

    2010-01-01

    This fourth edition of the "ES Review" brings together, in one setting, some of the best work from 2009-10. It features: (1) Teacher Quality (Teachers at Work: Improving Teacher Quality Through School Design (Elena Silva); Understanding Teachers Contracts (Andrew J. Rotherham); How Teachers Unions Lost the Media (Richard Whitmire and Andrew J.…

  19. Graphs for Isotopes of 99-Es (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides a graphic representation of nucleon separation energies and residual interaction parameters for isotopes of the chemical element 99-Es (Einsteinium, atomic number Z = 99).

  20. PRC2 complexes with JARID2, MTF2, and esPRC2p48 in ES cells to modulate ES cell pluripotency and somatic cell reprogramming.

    PubMed

    Zhang, Zhuo; Jones, Amanda; Sun, Chiao-Wang; Li, Chao; Chang, Chia-Wei; Joo, Heui-Yun; Dai, Qian; Mysliwiec, Matthew R; Wu, Li-Chen; Guo, Yahong; Yang, Wei; Liu, Kaimao; Pawlik, Kevin M; Erdjument-Bromage, Hediye; Tempst, Paul; Lee, Youngsook; Min, Jinrong; Townes, Tim M; Wang, Hengbin

    2011-02-01

    Polycomb repressive complex two (PRC2) has been implicated in embryonic stem (ES) cell pluripotency; however, the mechanistic roles of this complex are unclear. It was assumed that ES cells contain PRC2 with the same subunit composition as that identified in HeLa cells and Drosophila embryos. Here, we report that PRC2 in mouse ES cells contains at least three additional subunits: JARID2, MTF2, and a novel protein denoted esPRC2p48. JARID2, MTF2, and esPRC2p48 are highly expressed in mouse ES cells compared to differentiated cells. Importantly, knockdowns of JARID2, MTF2, or esPRC2p48 alter the level of PRC2-mediated H3K27 methylation and result in the expression of differentiation-associated genes in ES cells. Interestingly, expression of JARID2, MTF2, and esPRC2p48 together, but not individually, enhances Oct4/Sox2/Klf4-mediated reprogramming of mouse embryonic fibroblasts (MEFs) into induced pluripotent stem cells, whereas knockdown or knockout of JARID2, MTF2, or esPRC2p48 significantly inhibits reprogramming. JARID2, MTF2, and esPRC2p48 modulate H3K27 methylation and facilitate repression of lineage-associated gene expression when transduced into MEFs, and synergistically stimulate the histone methyltransferase activity of PRC2 in vitro. Therefore, these studies identify JARID2, MTF2, and esPRC2p48 as important regulatory subunits of PRC2 in ES cells and reveal critical functions of these subunits in modulating PRC2's activity and gene expression both in ES cells and during somatic cell reprogramming.

  1. [Fourcroy and the "Lycĕes"].

    PubMed

    Viel, Claude

    2011-04-01

    In XVIIIth century on Encyclopaedists influence, the study of Sciences arouse a real passion. Many courses are proposed. Two private institutions are opened which are called "Lycées": "Lycée de la rue de Valois" and "Lycée des Arts". Fourcroy and Lavoisier have been influential members of these two. Problems come to "Lycée de la rue de Valois", lead to the "purge ballot" and to Lavoisier's eviction. In France, it is the "Terror" and Lavoisier few months after was guillotined as "Fermier general". The historic events exonerate Fourcroy, member of the Convention, to the accusation that he has no made steps to save Lavoisier. This important historic event is a part of this study. As we show in this study "Lycées" are been small universities and have contributed to maintenance bonds between French scientists at the Revolution. The "Musée de Paris" and the "Société Philomathique de Paris" are also evoked.

  2. Geometrical parameters of E+S pairs

    NASA Technical Reports Server (NTRS)

    Rampazzo, Roberto; Sulentic, Jack W.

    1990-01-01

    Local environmental conditions (i.e., density and angular momentum properties of protogalactic clouds) are thought to be factors affecting the ultimate morphology of a galaxy. The existence of significant numbers of mixed morphology (E/SO+S) pairs of galaxies would represent a direct challenge to this idea unless all early-type components are formed by mergers. The authors wished to isolate candidate E+S pairs for detailed study. The authors have observed 22 pairs of mixed morphology galaxies (containing at least one early-type component) selected from a catalog of Sulentic (1988: unpublished) based upon the ESO sky survey. The observed sample and relevant morphological and interaction characteristics are summarized in tabular form. The authors report the relevant geometrical properties of the galaxies in another table. They list the maximum values measured for the ellipticity and the a(4)/a shape parameter together with the total measured twisting along the profile beyond the seeing disk (they set an inner limit of 3 arcsed). An asterisk indicates objects in which a(4)/a is neither predominantly boxy nor disky. They found a large number of true mixed pairs with 13/22 E+S pairs in the present sample. The remaining objects include 5 disk pairs (composed of SO and S members) and 3 early-type pairs comprising E and SO members. They estimate that between 25 and 50 percent of the pairs in any complete sample will be of the E+S type. This suggests that 100 to 200 such pairs exist on the sky brighter than m sub pg = 16.0. They found no global evidence for a difference between E members of this sample and those in more general samples (e.g., Bender et al. 1989). In particular, they found that about 30 percent of the early-type galaxies cannot be classified either predominantly boxy or disky because the a(4)/a profile shows both of these features at a comparable level or does not show any significant trend. Isophotal twisting is observed with a range and distribution

  3. Polyethylene glycol-electrolyte solution (PEG-ES)

    MedlinePlus

    Polyethylene glycol-electrolyte solution (PEG-ES) is used to empty the colon (large intestine, bowel) before a ... Polyethylene glycol-electrolyte solution (PEG-ES) comes as a powder to mix with water and take by ...

  4. PHOEBE: PHysics Of Eclipsing BinariEs

    NASA Astrophysics Data System (ADS)

    Prsa, Andrej; Matijevic, Gal; Latkovic, Olivera; Vilardell, Francesc; Wils, Patrick

    2011-06-01

    PHOEBE (PHysics Of Eclipsing BinariEs) is a modeling package for eclipsing binary stars, built on top of the widely used WD program (Wilson & Devinney 1971). This introductory paper overviews most important scientific extensions (incorporating observational spectra of eclipsing binaries into the solution-seeking process, extracting individual temperatures from observed color indices, main-sequence constraining and proper treatment of the reddening), numerical innovations (suggested improvements to WD's Differential Corrections method, the new Nelder & Mead's downhill Simplex method) and technical aspects (back-end scripter structure, graphical user interface). While PHOEBE retains 100% WD compatibility, its add-ons are a powerful way to enhance WD by encompassing even more physics and solution reliability.

  5. Abnormal fb Es enhancements in equatorial Es layers during magnetic storms of solar cycle 23

    NASA Astrophysics Data System (ADS)

    Resende, L. C. A.; Denardini, C. M.; Batista, I. S.

    2013-09-01

    We have analyzed the behavior of blanketing frequency of the Es layer (fb Es) occurring at an equatorial station covering the days before, during and subsequent to 24 intense and very intense magnetic storms (Dst≤-100 nT) that occurred during the solar cycle 23. The fb Es was measured by digital ionosonde over São Luís, Brazil (2.33° S, 44.2° W, dip: -4.5°). Our analysis shows that there are significant changes in the fb Es, mainly during the recovery phase of magnetic storms, characterized by occurrence of peaks that exceed the ambient background values. Also, these peaks are associated to other types of sporadic E layer than the Esq (a non-blanketing layer detected due the plasma irregularities in the equatorial electrojet), which in turn means competing mechanisms. The results are discussed in terms of the statistics of the abnormal enhancement taking into account the phase of the magnetic storm.

  6. CERES ERBE-like Monthly Regional Averages (ES-9) in HDF (CER_ES9_TRMM-PFM_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Regional Averages (ES-9) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-9 is also produced for combinations of scanner instruments. All instantaneous shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-8 product for a month are sorted by 2.5-degree spatial regions, by day number, and by the local hour of observation. The mean of the instantaneous fluxes for a given region-day-hour bin is determined and recorded on the ES-9 along with other flux statistics and scene information. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The ES-9 also contains hourly average fluxes for the month and an overall monthly average for each region. These average fluxes are given for both clear-sky and total-sky scenes. The following CERES ES9 data sets are currently available: CER_ES9_FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition2 CER_ES9_PFM+FM1_Edition1 CER_ES9_PFM+FM2_Edition1 CER_ES9_PFM+FM1_Edition2 CER_ES9_PFM+FM2_Edition2 CER_ES9_TRMM-PFM_Edition1 CER_ES9_TRMM-PFM_Edition2 CER_ES9_Terra-FM1_Edition1 CER_ES9_Terra-FM2_Edition1 CER_ES9_FM1+FM2_Edition2 CER_ES9_Terra-FM1_Edition2 CER_ES9_Terra-FM2_Edition2 CER_ES9_Aqua-FM3_Edition1 CER_ES9_Aqua-FM4_Edition1 CER_ES9_FM1+FM2+FM3+FM4_Edition1 CER_ES9_Aqua-FM3_Edition2 CER_ES9_Aqua-FM4_Edition2 CER_ES9_FM1+FM3_Edition2 CER_ES9_FM1+FM4_Edition2 CER_ES9_Aqua-FM3_Edition1-CV CER_ES9_Aqua-FM4_Edition1-CV CER_ES9_Terra-FM1_Edition1-CV CER_ES9_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2000-03-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost

  7. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES4_TRMM-PFM_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2000-03-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal

  8. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_TRMM-PFM_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2000-03-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  9. Earth System Documentation (ES-DOC) Preparation for CMIP6

    NASA Astrophysics Data System (ADS)

    Denvil, S.; Murphy, S.; Greenslade, M. A.; Lawrence, B.; Guilyardi, E.; Pascoe, C.; Treshanksy, A.; Elkington, M.; Hibling, E.; Hassell, D.

    2015-12-01

    During the course of 2015 the Earth System Documentation (ES-DOC) project began its preparations for CMIP6 (Coupled Model Inter-comparison Project 6) by further extending the ES-DOC tooling ecosystem in support of Earth System Model (ESM) documentation creation, search, viewing & comparison. The ES-DOC online questionnaire, the ES-DOC desktop notebook, and the ES-DOC python toolkit will serve as multiple complementary pathways to generating CMIP6 documentation. It is envisaged that institutes will leverage these tools at different points of the CMIP6 lifecycle. Institutes will be particularly interested to know that the documentation burden will be either streamlined or completely automated.As all the tools are tightly integrated with the ES-DOC web-service, institutes can be confident that the latency between documentation creation & publishing will be reduced to a minimum. Published documents will be viewable with the online ES-DOC Viewer (accessible via citable URL's). Model inter-comparison scenarios will be supported using the ES-DOC online Comparator tool. The Comparator is being extended to:• Support comparison of both Model descriptions & Simulation runs;• Greatly streamline the effort involved in compiling official tables.The entire ES-DOC ecosystem is open source and built upon open standards such as the Common Information Model (CIM) (versions 1 and 2).

  10. An empirical model of the midlatitude Es layer. IV

    NASA Astrophysics Data System (ADS)

    Ovezgeldyev, O. G.; Mikhailova, G. V.

    It is noted that when the frequency parameters of the sporadic E exceed the critical values of the regular layers of the ionosphere, Es permits radio transmissions at higher frequencies. To emphasize the importance of Es, it is also pointed out that in shielding the overlying region of the ionosphere, Es is often responsible for breaks in transmissions at shortwave frequencies through the regular layers of the ionosphere and for multipath errors. Attention is given here to the dependence on longitude and latitude of the expansion coefficients developed in previous studies of Es. It is pointed out that the horizontal component of the geomagnetic field, together with dynamic and photochemical factors, figures prominently in the makeup of the midlatitude Es. For this reason, the dependence on latitude of the expansion coefficients is, in general, complex. The dependence on longitude is thought to be much less pronounced, arising principally from the lack of coincidence of the geographic and geomagnetic coordinates.

  11. REAR AND SIDE OF PACIFIC COAST GARRISON MONUMENT, SECTION ES ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    REAR AND SIDE OF PACIFIC COAST GARRISON MONUMENT, SECTION ES (EAST SIDE), WITH ADMINISTRATION BUILDING AT RIGHT IN BACKGROUND. VIEW TO NORTH. - San Francisco National Cemetery, 1 Lincoln Boulevard, San Francisco, San Francisco County, CA

  12. Home Performance with Energy Star (HPwES) Program Report

    SciTech Connect

    none,

    2013-01-01

    This report summarizes the Department’s review of comments received on the HPwES v2 proposal and presents a multi-year action plan to both address Department goals and incorporate industry feedback.

  13. Final Technical Report: Electronic Structure Workshop (ES13)

    SciTech Connect

    Zhang, Shiwei

    2015-02-26

    The 25th Annual Workshop on Recent Developments in Electronic Structure Methods (ES2013) was successfully held at the College of William & Mary in Williamsburg VA on June 11-14, 2013. The workshop website is at http://es13.wm.edu/ , which contains updated information on the workshop and a permanent archive of the scientific contents. DOE's continued support has been instrumental to the success of the workshop.

  14. The Earth System Documentation (ES-DOC) Software Process

    NASA Astrophysics Data System (ADS)

    Greenslade, M. A.; Murphy, S.; Treshansky, A.; DeLuca, C.; Guilyardi, E.; Denvil, S.

    2013-12-01

    Earth System Documentation (ES-DOC) is an international project supplying high-quality tools & services in support of earth system documentation creation, analysis and dissemination. It is nurturing a sustainable standards based documentation eco-system that aims to become an integral part of the next generation of exa-scale dataset archives. ES-DOC leverages open source software, and applies a software development methodology that places end-user narratives at the heart of all it does. ES-DOC has initially focused upon nurturing the Earth System Model (ESM) documentation eco-system and currently supporting the following projects: * Coupled Model Inter-comparison Project Phase 5 (CMIP5); * Dynamical Core Model Inter-comparison Project (DCMIP); * National Climate Predictions and Projections Platforms Quantitative Evaluation of Downscaling Workshop. This talk will demonstrate that ES-DOC implements a relatively mature software development process. Taking a pragmatic Agile process as inspiration, ES-DOC: * Iteratively develops and releases working software; * Captures user requirements via a narrative based approach; * Uses online collaboration tools (e.g. Earth System CoG) to manage progress; * Prototypes applications to validate their feasibility; * Leverages meta-programming techniques where appropriate; * Automates testing whenever sensibly feasible; * Streamlines complex deployments to a single command; * Extensively leverages GitHub and Pivotal Tracker; * Enforces strict separation of the UI from underlying API's; * Conducts code reviews.

  15. [And Sarah Félix created "L 'Eau des Fées" and "La Parfumerie des Fées"].

    PubMed

    Raynal, Cécile

    2015-12-01

    century, the parisian perfumery of Sarah Felix was famous especially for her cosmetic "L'Eau des Fées" ("Water of the Fairies"), a dyeing supposed to give back to the old hair the color of their youth. Sarah Felix, sister of the famous actress Rachel, exercised several jobs before becoming perfumer. At first, she was actress, then she tried ... oyster culture in Normandy ! Abandoning these activities, Sarah Felix became really a businesswoman in Paris. She dedicated herself to perfumery, in particular to "L'Eau des Fées", from 1866. To launch its product, she was associated to competency characters (engineer, pharmacist, physician) and benefited from the protection of the imperial family. A lawsuit between the associates and the 1870 war hindered the company's early days, but "L'Eau des Fées" provided with quality pledges, imposed itself definitely. The "Parfumerie des Fées" completed the range of its cosmetics with creams, powders, etc. Sarah Félix paid attention to present "L'Eau des Fées" as a dye devoid of toxicity. Was it really the case and could il be sold nowadays? These two issues are discussed in the last part. PMID:26827551

  16. [And Sarah Félix created "L 'Eau des Fées" and "La Parfumerie des Fées"].

    PubMed

    Raynal, Cécile

    2015-12-01

    century, the parisian perfumery of Sarah Felix was famous especially for her cosmetic "L'Eau des Fées" ("Water of the Fairies"), a dyeing supposed to give back to the old hair the color of their youth. Sarah Felix, sister of the famous actress Rachel, exercised several jobs before becoming perfumer. At first, she was actress, then she tried ... oyster culture in Normandy ! Abandoning these activities, Sarah Felix became really a businesswoman in Paris. She dedicated herself to perfumery, in particular to "L'Eau des Fées", from 1866. To launch its product, she was associated to competency characters (engineer, pharmacist, physician) and benefited from the protection of the imperial family. A lawsuit between the associates and the 1870 war hindered the company's early days, but "L'Eau des Fées" provided with quality pledges, imposed itself definitely. The "Parfumerie des Fées" completed the range of its cosmetics with creams, powders, etc. Sarah Félix paid attention to present "L'Eau des Fées" as a dye devoid of toxicity. Was it really the case and could il be sold nowadays? These two issues are discussed in the last part.

  17. Certification testing for the ES-2 shipping package

    SciTech Connect

    Feldman, M.R.; Byington, G.A.; Handy, K.D.; Shappert, L.B.; Handy, K.D.; Oaks, R.E. Jr.; Stumpfl, E.

    1998-02-01

    The ES-2 is a multiconfiguration, Type B fissile material shipping package, designed by the Y-12 Nuclear Packaging Systems. It is unique in that a castable refractory material performs primary impact absorption and thermal insulation duties. This material, unlike the insulation often used in fissile material packages, such as Celotex and various foams, is fireproof at temperatures associated with Type B package testing (800 C). The ES-2 is designed to permit the use of three different containment vessels which can result in as many as six different configurations. Eight prototype units were manufactured and successfully tested to US Federal Regulatory Requirements.

  18. Electron-capture delayed fission in {sup 246}Es

    SciTech Connect

    Shaughnessy, D.A.; Gregorich, K.E.; Hendricks, M.B.; Lane, M.R.

    1997-12-31

    We have extended our systematic study of electron-capture delayed fission (ECDF) in neutron-deficient isotopes to {sup 246}Es. The {sup 246}Es was produced at the 88 Inch Cyclotron at Lawrence Berkeley National Laboratory via the {sup 249}Cf(p,xn){sup 250-x}Es reaction with 37 MeV protons. There were 19 {sup 249}Cf targets used simultaneously in our light ion multiple (LIM) target system. Alpha particles and fission fragments were detected in our rotating wheel system. In some experiments, TTA extractions were performed to remove interfering activities. The chemically separated samples were positioned between a solid-state particle detector and two x-rays detectors. This configuration enabled us to look for fissions in coincidence with K x-rays following electron-capture. Our measured production cross section of 13 {+-} 5 {mu}b for {sup 246}Es was much lower than the cross section predicted by a neutron evaporation code. The probability of delayed fission was determined from the number of x-ray/fission coincidences measured.

  19. Stereotyping in "Damals war es Friedrich" and "Brandstiftung."

    ERIC Educational Resources Information Center

    Moffit, Gisela

    2000-01-01

    Discusses the use of young adult books in teaching high school and college-level German, particularly "Damals war es Friedrich" and "Brandstiftung." The didactic intentions of these books are to inform younger adults about the Nazi periods of the past and the Neo-nazi resurgence of the present, encourage them to confront the issues, and to take a…

  20. 1ES 1927+654: a bare Seyfert 2

    NASA Astrophysics Data System (ADS)

    Gallo, L. C.; MacMackin, C.; Vasudevan, R.; Cackett, E. M.; Fabian, A. C.; Panessa, F.

    2013-07-01

    1ES 1927+654 is an active galactic nucleus (AGN) that appears to defy the unification model. It exhibits a type-2 optical spectrum, but possesses little X-ray obscuration. XMM-Newton and Suzaku observations obtained in 2011 are used to study the X-ray properties of 1ES 1927+654. The spectral energy distribution derived from simultaneous optical-to-X-ray data obtained with XMM-Newton shows that the AGN has a typical Eddington ratio (L/LEdd = 0.014 - 0.11). The X-ray spectrum and rapid variability are consistent with originating from a corona surrounding a standard accretion disc. Partial covering models can describe the X-ray data; however, the narrow Fe Kα emission line predicted from standard photoelectric absorption is not detected. Ionized partial covering also favours a high-velocity outflow (v ≈ 0.3c), which requires the kinetic luminosity of the wind to be ≳30 per cent of the bolometric luminosity of the AGN. Such values are not unusual, but for 1ES 1927+654 it requires that the wind is launched very close to the black hole (˜10 rg). Blurred reflection models also work well at describing the spectral and timing properties of 1ES 1927+654 if the AGN is viewed nearly edge-on, implying that an inner accretion disc must be present. The high inclination is intriguing as it suggests 1ES 1927+654 could be orientated like a Seyfert 2, in agreement with its optical classification, but viewed through a tenuous torus.

  1. Purification and Characterization of AsES Protein

    PubMed Central

    Chalfoun, Nadia R.; Grellet-Bournonville, Carlos F.; Martínez-Zamora, Martín G.; Díaz-Perales, Araceli; Castagnaro, Atilio P.; Díaz-Ricci, Juan C.

    2013-01-01

    In this work, the purification and characterization of an extracellular elicitor protein, designated AsES, produced by an avirulent isolate of the strawberry pathogen Acremonium strictum, are reported. The defense eliciting activity present in culture filtrates was recovered and purified by ultrafiltration (cutoff, 30 kDa), anionic exchange (Q-Sepharose, pH 7.5), and hydrophobic interaction (phenyl-Sepharose) chromatographies. Two-dimensional SDS-PAGE of the purified active fraction revealed a single spot of 34 kDa and pI 8.8. HPLC (C2/C18) and MS/MS analysis confirmed purification to homogeneity. Foliar spray with AsES provided a total systemic protection against anthracnose disease in strawberry, accompanied by the expression of defense-related genes (i.e. PR1 and Chi2-1). Accumulation of reactive oxygen species (e.g. H2O2 and O2˙̄) and callose was also observed in Arabidopsis. By using degenerate primers designed from the partial amino acid sequences and rapid amplification reactions of cDNA ends, the complete AsES-coding cDNA of 1167 nucleotides was obtained. The deduced amino acid sequence showed significant identity with fungal serine proteinases of the subtilisin family, indicating that AsES is synthesized as a larger precursor containing a 15-residue secretory signal peptide and a 90-residue peptidase inhibitor I9 domain in addition to the 283-residue mature protein. AsES exhibited proteolytic activity in vitro, and its resistance eliciting activity was eliminated when inhibited with PMSF, suggesting that its proteolytic activity is required to induce the defense response. This is, to our knowledge, the first report of a fungal subtilisin that shows eliciting activity in plants. This finding could contribute to develop disease biocontrol strategies in plants by activating its innate immunity. PMID:23530047

  2. CERES ERBE-like Monthly Regional Averages (ES-9) in HDF ( CER_ES9_Terra-FM2_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Regional Averages (ES-9) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-9 is also produced for combinations of scanner instruments. All instantaneous shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-8 product for a month are sorted by 2.5-degree spatial regions, by day number, and by the local hour of observation. The mean of the instantaneous fluxes for a given region-day-hour bin is determined and recorded on the ES-9 along with other flux statistics and scene information. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The ES-9 also contains hourly average fluxes for the month and an overall monthly average for each region. These average fluxes are given for both clear-sky and total-sky scenes. The following CERES ES9 data sets are currently available: CER_ES9_FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition2 CER_ES9_PFM+FM1_Edition1 CER_ES9_PFM+FM2_Edition1 CER_ES9_PFM+FM1_Edition2 CER_ES9_PFM+FM2_Edition2 CER_ES9_TRMM-PFM_Edition1 CER_ES9_TRMM-PFM_Edition2 CER_ES9_Terra-FM1_Edition1 CER_ES9_Terra-FM2_Edition1 CER_ES9_FM1+FM2_Edition2 CER_ES9_Terra-FM1_Edition2 CER_ES9_Terra-FM2_Edition2 CER_ES9_Aqua-FM3_Edition1 CER_ES9_Aqua-FM4_Edition1 CER_ES9_FM1+FM2+FM3+FM4_Edition1 CER_ES9_Aqua-FM3_Edition2 CER_ES9_Aqua-FM4_Edition2 CER_ES9_FM1+FM3_Edition2 CER_ES9_FM1+FM4_Edition2 CER_ES9_Aqua-FM3_Edition1-CV CER_ES9_Aqua-FM4_Edition1-CV CER_ES9_Terra-FM1_Edition1-CV CER_ES9_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2006-10-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost

  3. CERES ERBE-like Monthly Regional Averages (ES-9) in HDF (CERES:CER_ES9_PFM+FM1_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Regional Averages (ES-9) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-9 is also produced for combinations of scanner instruments. All instantaneous shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-8 product for a month are sorted by 2.5-degree spatial regions, by day number, and by the local hour of observation. The mean of the instantaneous fluxes for a given region-day-hour bin is determined and recorded on the ES-9 along with other flux statistics and scene information. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The ES-9 also contains hourly average fluxes for the month and an overall monthly average for each region. These average fluxes are given for both clear-sky and total-sky scenes. The following CERES ES9 data sets are currently available: CER_ES9_FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition2 CER_ES9_PFM+FM1_Edition1 CER_ES9_PFM+FM2_Edition1 CER_ES9_PFM+FM1_Edition2 CER_ES9_PFM+FM2_Edition2 CER_ES9_TRMM-PFM_Edition1 CER_ES9_TRMM-PFM_Edition2 CER_ES9_Terra-FM1_Edition1 CER_ES9_Terra-FM2_Edition1 CER_ES9_FM1+FM2_Edition2 CER_ES9_Terra-FM1_Edition2 CER_ES9_Terra-FM2_Edition2 CER_ES9_Aqua-FM3_Edition1 CER_ES9_Aqua-FM4_Edition1 CER_ES9_FM1+FM2+FM3+FM4_Edition1 CER_ES9_Aqua-FM3_Edition2 CER_ES9_Aqua-FM4_Edition2 CER_ES9_FM1+FM3_Edition2 CER_ES9_FM1+FM4_Edition2 CER_ES9_Aqua-FM3_Edition1-CV CER_ES9_Aqua-FM4_Edition1-CV CER_ES9_Terra-FM1_Edition1-CV CER_ES9_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2000-03-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost

  4. CERES ERBE-like Monthly Regional Averages (ES-9) in HDF (CER_ES9_PFM+FM1+FM2_Edition1)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Regional Averages (ES-9) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-9 is also produced for combinations of scanner instruments. All instantaneous shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-8 product for a month are sorted by 2.5-degree spatial regions, by day number, and by the local hour of observation. The mean of the instantaneous fluxes for a given region-day-hour bin is determined and recorded on the ES-9 along with other flux statistics and scene information. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The ES-9 also contains hourly average fluxes for the month and an overall monthly average for each region. These average fluxes are given for both clear-sky and total-sky scenes. The following CERES ES9 data sets are currently available: CER_ES9_FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition2 CER_ES9_PFM+FM1_Edition1 CER_ES9_PFM+FM2_Edition1 CER_ES9_PFM+FM1_Edition2 CER_ES9_PFM+FM2_Edition2 CER_ES9_TRMM-PFM_Edition1 CER_ES9_TRMM-PFM_Edition2 CER_ES9_Terra-FM1_Edition1 CER_ES9_Terra-FM2_Edition1 CER_ES9_FM1+FM2_Edition2 CER_ES9_Terra-FM1_Edition2 CER_ES9_Terra-FM2_Edition2 CER_ES9_Aqua-FM3_Edition1 CER_ES9_Aqua-FM4_Edition1 CER_ES9_FM1+FM2+FM3+FM4_Edition1 CER_ES9_Aqua-FM3_Edition2 CER_ES9_Aqua-FM4_Edition2 CER_ES9_FM1+FM3_Edition2 CER_ES9_FM1+FM4_Edition2 CER_ES9_Aqua-FM3_Edition1-CV CER_ES9_Aqua-FM4_Edition1-CV CER_ES9_Terra-FM1_Edition1-CV CER_ES9_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2000-03-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost

  5. CERES ERBE-like Monthly Regional Averages (ES-9) in HDF (CER_ES9_FM1+FM3_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Regional Averages (ES-9) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-9 is also produced for combinations of scanner instruments. All instantaneous shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-8 product for a month are sorted by 2.5-degree spatial regions, by day number, and by the local hour of observation. The mean of the instantaneous fluxes for a given region-day-hour bin is determined and recorded on the ES-9 along with other flux statistics and scene information. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The ES-9 also contains hourly average fluxes for the month and an overall monthly average for each region. These average fluxes are given for both clear-sky and total-sky scenes. The following CERES ES9 data sets are currently available: CER_ES9_FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition2 CER_ES9_PFM+FM1_Edition1 CER_ES9_PFM+FM2_Edition1 CER_ES9_PFM+FM1_Edition2 CER_ES9_PFM+FM2_Edition2 CER_ES9_TRMM-PFM_Edition1 CER_ES9_TRMM-PFM_Edition2 CER_ES9_Terra-FM1_Edition1 CER_ES9_Terra-FM2_Edition1 CER_ES9_FM1+FM2_Edition2 CER_ES9_Terra-FM1_Edition2 CER_ES9_Terra-FM2_Edition2 CER_ES9_Aqua-FM3_Edition1 CER_ES9_Aqua-FM4_Edition1 CER_ES9_FM1+FM2+FM3+FM4_Edition1 CER_ES9_Aqua-FM3_Edition2 CER_ES9_Aqua-FM4_Edition2 CER_ES9_FM1+FM3_Edition2 CER_ES9_FM1+FM4_Edition2 CER_ES9_Aqua-FM3_Edition1-CV CER_ES9_Aqua-FM4_Edition1-CV CER_ES9_Terra-FM1_Edition1-CV CER_ES9_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2005-12-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost

  6. CERES ERBE-like Monthly Regional Averages (ES-9) in HDF (CER_ES9_FM1+FM2_Edition1)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Regional Averages (ES-9) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-9 is also produced for combinations of scanner instruments. All instantaneous shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-8 product for a month are sorted by 2.5-degree spatial regions, by day number, and by the local hour of observation. The mean of the instantaneous fluxes for a given region-day-hour bin is determined and recorded on the ES-9 along with other flux statistics and scene information. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The ES-9 also contains hourly average fluxes for the month and an overall monthly average for each region. These average fluxes are given for both clear-sky and total-sky scenes. The following CERES ES9 data sets are currently available: CER_ES9_FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition2 CER_ES9_PFM+FM1_Edition1 CER_ES9_PFM+FM2_Edition1 CER_ES9_PFM+FM1_Edition2 CER_ES9_PFM+FM2_Edition2 CER_ES9_TRMM-PFM_Edition1 CER_ES9_TRMM-PFM_Edition2 CER_ES9_Terra-FM1_Edition1 CER_ES9_Terra-FM2_Edition1 CER_ES9_FM1+FM2_Edition2 CER_ES9_Terra-FM1_Edition2 CER_ES9_Terra-FM2_Edition2 CER_ES9_Aqua-FM3_Edition1 CER_ES9_Aqua-FM4_Edition1 CER_ES9_FM1+FM2+FM3+FM4_Edition1 CER_ES9_Aqua-FM3_Edition2 CER_ES9_Aqua-FM4_Edition2 CER_ES9_FM1+FM3_Edition2 CER_ES9_FM1+FM4_Edition2 CER_ES9_Aqua-FM3_Edition1-CV CER_ES9_Aqua-FM4_Edition1-CV CER_ES9_Terra-FM1_Edition1-CV CER_ES9_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2003-12-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost

  7. CERES ERBE-like Monthly Regional Averages (ES-9) in HDF (CER_ES9_FM1+FM4_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Regional Averages (ES-9) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-9 is also produced for combinations of scanner instruments. All instantaneous shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-8 product for a month are sorted by 2.5-degree spatial regions, by day number, and by the local hour of observation. The mean of the instantaneous fluxes for a given region-day-hour bin is determined and recorded on the ES-9 along with other flux statistics and scene information. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The ES-9 also contains hourly average fluxes for the month and an overall monthly average for each region. These average fluxes are given for both clear-sky and total-sky scenes. The following CERES ES9 data sets are currently available: CER_ES9_FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition2 CER_ES9_PFM+FM1_Edition1 CER_ES9_PFM+FM2_Edition1 CER_ES9_PFM+FM1_Edition2 CER_ES9_PFM+FM2_Edition2 CER_ES9_TRMM-PFM_Edition1 CER_ES9_TRMM-PFM_Edition2 CER_ES9_Terra-FM1_Edition1 CER_ES9_Terra-FM2_Edition1 CER_ES9_FM1+FM2_Edition2 CER_ES9_Terra-FM1_Edition2 CER_ES9_Terra-FM2_Edition2 CER_ES9_Aqua-FM3_Edition1 CER_ES9_Aqua-FM4_Edition1 CER_ES9_FM1+FM2+FM3+FM4_Edition1 CER_ES9_Aqua-FM3_Edition2 CER_ES9_Aqua-FM4_Edition2 CER_ES9_FM1+FM3_Edition2 CER_ES9_FM1+FM4_Edition2 CER_ES9_Aqua-FM3_Edition1-CV CER_ES9_Aqua-FM4_Edition1-CV CER_ES9_Terra-FM1_Edition1-CV CER_ES9_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2005-03-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost

  8. CERES ERBE-like Monthly Regional Averages (ES-9) in HDF (CER_ES9_Terra-FM1_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Regional Averages (ES-9) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-9 is also produced for combinations of scanner instruments. All instantaneous shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-8 product for a month are sorted by 2.5-degree spatial regions, by day number, and by the local hour of observation. The mean of the instantaneous fluxes for a given region-day-hour bin is determined and recorded on the ES-9 along with other flux statistics and scene information. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The ES-9 also contains hourly average fluxes for the month and an overall monthly average for each region. These average fluxes are given for both clear-sky and total-sky scenes. The following CERES ES9 data sets are currently available: CER_ES9_FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition1 CER_ES9_PFM+FM1+FM2_Edition2 CER_ES9_PFM+FM1_Edition1 CER_ES9_PFM+FM2_Edition1 CER_ES9_PFM+FM1_Edition2 CER_ES9_PFM+FM2_Edition2 CER_ES9_TRMM-PFM_Edition1 CER_ES9_TRMM-PFM_Edition2 CER_ES9_Terra-FM1_Edition1 CER_ES9_Terra-FM2_Edition1 CER_ES9_FM1+FM2_Edition2 CER_ES9_Terra-FM1_Edition2 CER_ES9_Terra-FM2_Edition2 CER_ES9_Aqua-FM3_Edition1 CER_ES9_Aqua-FM4_Edition1 CER_ES9_FM1+FM2+FM3+FM4_Edition1 CER_ES9_Aqua-FM3_Edition2 CER_ES9_Aqua-FM4_Edition2 CER_ES9_FM1+FM3_Edition2 CER_ES9_FM1+FM4_Edition2 CER_ES9_Aqua-FM3_Edition1-CV CER_ES9_Aqua-FM4_Edition1-CV CER_ES9_Terra-FM1_Edition1-CV CER_ES9_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2005-12-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost

  9. Cloning of ES cells and mice by nuclear transfer.

    PubMed

    Wakayama, Sayaka; Kishigami, Satoshi; Wakayama, Teruhiko

    2009-01-01

    We have been able to develop a stable nuclear transfer (NT) method in the mouse, in which donor nuclei are directly injected into the oocyte using a piezo-actuated micromanipulator. Although the piezo unit is a complex tool, once mastered it is of great help not only in NT experiments, but also in almost all other forms of micromanipulation. Using this technique, embryonic stem (ntES) cell lines established from somatic cell nuclei can be generated relatively easily from a variety of mouse genotypes and cell types. Such ntES cells can be used not only for experimental models of human therapeutic cloning but also as a means of preserving mouse genomes instead of preserving germ cells. Here, we describe our most recent protocols for mouse cloning.

  10. Coherent structures in the Es layer and neutral middle atmosphere

    NASA Astrophysics Data System (ADS)

    Mošna, Zbyšek; Knížová, Petra Koucká; Potužníková, Kateřina

    2015-12-01

    The present paper shows results from the summer campaign performed during geomagnetically quiet period from June 1 to August 31, 2009. Within time-series of stratospheric and mesospheric temperatures at pressure levels 10-0.1 hPa, mesospheric winds measured in Collm, Germany, and the sporadic E-layer parameters foEs and hEs measured at the Pruhonice station we detected specific coherent wave-bursts in planetary wave domain. Permanent wave-like activity is observed in all analyzed data sets. However, the number of wave-like structures persistent in large range of height from the stratosphere to lower ionosphere is limited. The only coherent modes that are detected on consequent levels of the atmosphere are those corresponding to eigenmodes of planetary waves.

  11. Neutron capture and fission in /sup 254g/ Es

    SciTech Connect

    Halperin, J.; Bigelow, J.E.; O'Kelley, G.D.; Oliver, J.H.; Wiggins, J.T.

    1985-07-01

    Integral neutron capture and neutron fission cross sections have been measured for the 276-day /sup 254g/ Es. Thermal cross sections and resonance integrals were evaluated using a cadmium filter technique. Capture cross sections were determined from alpha-particle spectrum measurements following neutron irradiations with cobalt flux monitors. Fission cross sections were measured using fission track detection techniques with STTU monitors. The fission cross-section values compared favorably with an absorption cross-section determination from a burnout experiment of SVTEs-SVUEs. The integral neutron capture and fission cross sections determined for /sup 254g/ Es are: sigma /sub c/ /sup th/ = 28.3 + or - 2.5 and I /sub c/ = 18.2 + or - 1.5 b, and sigma /sub F/ /sup th/ = 1970 + or - 200 and I /sub F/ = 1200 + or - 250 b.

  12. Electron-capture delayed fission properties of 244Es

    SciTech Connect

    Shaughnessy, Dawn A.; Gregorich, Kenneth E.; Adams, Jeb L.; Lane, Michael R.; Laue, Carola A.; Lee, Diana M.; McGrath, Christopher A.; Ninov, Victor; Patin, Joshua B.; Strellis, Dan A.; Sylwester, Eric R.; Wilk, Philip A.; Hoffman, Darleane C.

    2001-03-16

    Electron-capture delayed fission was observed in {sup 244}Es produced via the {sup 237}Np({sup 12}C,5n){sup 244}Es reaction at 81 MeV (on target) with a production cross section of 0.31{+-}0.12 {micro}b. The mass-yield distribution of the fission fragments is highly asymmetric. The average preneutron-emission total kinetic energy of the fragments was measured to be 186{+-}19 MeV. Based on the ratio of the number of fission events to the measured number of {alpha} decays from the electron-capture daughter {sup 244}Cf (100% {alpha} branch), the probability of delayed fission was determined to be (1.2{+-}0.4) x 10{sup -4}. This value for the delayed fission probability fits the experimentally observed trend of increasing delayed fission probability with increasing Q value for electron-capture.

  13. Generation of Gene Knockout Mice by ES Cell Microinjection

    PubMed Central

    Longenecker, Glenn; Kulkarni, Ashok B

    2009-01-01

    This unit lists and describes protocols used in the production of chimeric mice leading to the generation of gene knockout mice. These protocols include the collection of blastocyst embryos, ES cell injection, and uterine transfer of injected blastocysts. Support protocols in the superovulation of blastocyst donor mice, generation of pseudopregnant recipients, fabrication of glass pipettes, and generation of germline mice are also included. Practical tips and solutions are mentioned to help troubleshoot problems that may occur. PMID:19731226

  14. Complete genome sequence of hyperthermophilic archaeon Thermococcus sp. ES1.

    PubMed

    Jung, Jong-Hyun; Kim, You-Tae; Jeon, Eun-Jung; Seo, Dong-Ho; Hensley, Sarah A; Holden, James F; Lee, Ju-Hoon; Park, Cheon-Seok

    2014-03-20

    Thermococcus sp. strain ES1 is an anaerobic, hyperthermophilic archaeon from a hydrothermal vent that catabolizes sugars and peptides and produces H2S from S°, H2, acetate and CO2 as its primary metabolites. We present the complete genome sequence of this strain (1,957,742bp) with a focus on its substrate utilization and metabolite production capabilities. The sequence will contribute to the development of heterotrophic archaea for bioenergy production and biogeochemical modeling in hydrothermal environments. PMID:24472758

  15. Electron-capture delayed fission properties of {sup 242}Es

    SciTech Connect

    Shaughnessy, D. A.; Adams, J. L.

    2000-04-01

    Electron-capture delayed fission of {sup 242}Es produced via the {sup 233}U({sup 14}N,5n){sup 242}Es reaction at 87 MeV (on target) was observed to decay with a half-life of 11{+-}3 s, consistent with the reported {alpha}-decay half-life of {sup 242}Es of 16{sub -4}{sup +6} s. The mass-yield distribution of the fission fragments is highly asymmetric. The average pre-neutron emission total kinetic energy of the fragments was measured to be 183{+-}18 MeV. Based on the ratio of the measured number of fission events to the measured number of {alpha} decays from the electron-capture daughter {sup 242}Cf (100% {alpha} branch), the probability of delayed fission was determined to be 0.006{+-}0.002. This value for the delayed fission probability fits the experimental trend of increasing delayed fission probability with increasing Q value for electron capture. (c) 2000 The American Physical Society.

  16. [History of the Journées Dermatologiques de Paris].

    PubMed

    Tilles, G

    2013-12-01

    Founded in 1801 at the Hôpital Saint-Louis, Paris, under Jean-Louis Alibert, the French School of Dermatology was initially structured around the French Society of Dermatology (1889) and the organization of two world congresses (Paris 1889, 1900). After World War I, the creation of dermatological societies in the provinces infused French dermatology with new energy. In 1922, the first congress of the French-speaking dermatologists further contributed to the public profile of dermatologists in France. The "Journées de Mars" were initiated in 1961 at the Hôpital Saint-Louis, and in 1975 they went on to become the "Journées dermatologiques de Paris". Pr. Jean Civatte played a key role in their creation and in their organization for 30 years. After 1979, since actual patients could no longer be presented, the organizers of the congress had to change the content of the meeting from clinical presentations to post-graduate teaching and clinical research. From its origins in the form of meetings of French dermatologists in an intimate setting at the Hôpital Saint-Louis, the "Journées dermatologiques de Paris" grew within the ensuing decades into a major scientific event of the French-speaking dermatological community, bringing together more than 4000 participants in December each year.

  17. APOSTLE: 11 TRANSIT OBSERVATIONS OF TrES-3b

    SciTech Connect

    Kundurthy, P.; Becker, A. C.; Agol, E.; Barnes, R.; Williams, B.

    2013-02-10

    The Apache Point Survey of Transit Lightcurves of Exoplanets (APOSTLE) observed 11 transits of TrES-3b over two years in order to constrain system parameters and look for transit timing and depth variations. We describe an updated analysis protocol for APOSTLE data, including the reduction pipeline, transit model, and Markov Chain Monte Carlo analyzer. Our estimates of the system parameters for TrES-3b are consistent with previous estimates to within the 2{sigma} confidence level. We improved the errors (by 10%-30%) on system parameters such as the orbital inclination (i {sub orb}), impact parameter (b), and stellar density ({rho}{sub *}) compared to previous measurements. The near-grazing nature of the system, and incomplete sampling of some transits, limited our ability to place reliable uncertainties on individual transit depths and hence we do not report strong evidence for variability. Our analysis of the transit timing data shows no evidence for transit timing variations and our timing measurements are able to rule out super-Earth and gas giant companions in low-order mean motion resonance with TrES-3b.

  18. Solar Energy Grid Integration Systems -- Energy Storage (SEGIS-ES).

    SciTech Connect

    Hanley, Charles J.; Ton, Dan T.; Boyes, John D.; Peek, Georgianne Huff

    2008-07-01

    This paper describes the concept for augmenting the SEGIS Program (an industry-led effort to greatly enhance the utility of distributed PV systems) with energy storage in residential and small commercial applications (SEGIS-ES). The goal of SEGIS-ES is to develop electrical energy storage components and systems specifically designed and optimized for grid-tied PV applications. This report describes the scope of the proposed SEGIS-ES Program and why it will be necessary to integrate energy storage with PV systems as PV-generated energy becomes more prevalent on the nation's utility grid. It also discusses the applications for which energy storage is most suited and for which it will provide the greatest economic and operational benefits to customers and utilities. Included is a detailed summary of the various storage technologies available, comparisons of their relative costs and development status, and a summary of key R&D needs for PV-storage systems. The report concludes with highlights of areas where further PV-specific R&D is needed and offers recommendations about how to proceed with their development.

  19. CERES ERBE-like Monthly Geographical Averages (ES-4) in HDF (CER_ES4_Terra-FM1_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2006-09-30] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal

  20. CERES ERBE-like Monthly Geographical Averages (ES-4) in HDF (CER_ES4_Aqua-FM3_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2006-10-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal

  1. CERES ERBE-like Monthly Geographical Averages (ES-4) in HDF (CER_ES4_PFM+FM2_Edition1)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2000-03-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal

  2. CERES ERBE-like Monthly Geographical Averages (ES-4) in HDF (CER_ES4_Terra-FM2_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2005-12-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal

  3. CERES ERBE-like Monthly Geographical Averages (ES-4) in HDF (CER_ES4_Terra-FM1_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2005-12-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal

  4. CERES ERBE-like Monthly Geographical Averages (ES-4) in HDF (CER_ES4_FM1+FM2+FM3+FM4_Edition1)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2003-12-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal

  5. CERES ERBE-like Monthly Geographical Averages (ES-4) in HDF (CER_ES4_Aqua-FM3_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2005-12-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal

  6. CERES ERBE-like Monthly Geographical Averages (ES-4) in HDF (CER_ES4_PFM+FM1_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2000-03-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal

  7. CERES ERBE-like Monthly Geographical Averages (ES-4) in HDF (CER_ES4_Terra-FM2_Edition1)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2005-10-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal

  8. CHRONIC ENTERITIS IN PATIENTS UNDERGOING PELVIC RADIOTHERAPY: PREVALENCE, RISK FACTORS AND ASSOCIATED COMPLICATIONS.

    PubMed

    Hernández-Moreno, Ana; Vidal-Casariego, Alfonso; Calleja-Fernández, Alicia; Kyriakos, Georgios; Villar-Taibo, Rocío; Urioste-Fondo, Ana; Cano-Rodríguez, Isidoro; Ballesteros-Pomar, María D

    2015-11-01

    Introducción: la radiacion de los tumores de la cavidad pelvica puede provocar mucositis a nivel intestinal. El objetivo de nuestro estudio fue determinar la prevalencia, los factores de riesgo y las complicaciones de la enteritis radica cronica en los pacientes tratados con radioterapia pelvica. Pacientes y métodos: estudio transversal sobre 150 pacientes tratados con radioterapia pelvica durante el ano 2008 debido a un cancer de prostata, cervix, endometrio o recto. Se interrogo a los pacientes sobre la presencia de sintomas sugestivos de enteritis y sobre cambios en el peso habitual y modificaciones en su dieta. Los parametros considerados como posibles factores de riesgo de enteritis cronica (sexo, edad, tratamiento antitumoral, enteritis aguda previa y tipo de tumor) se analizaron con metodos univariantes y multivariantes. Resultados: el estudio incluyo finalmente a 100 pacientes, el 84% varones, con una mediana de edad de 72,3 anos. Se encontro una prevalencia de enteritis radica cronica del 20%, en la mayoria de grado 1 (45%). Por otra parte, el 10% referian una perdida de peso ≥ 5 kg, el 3% habian requerido hospitalizacion debido a diarrea incoercible o a obstruccion intestinal, y el 11% habian modificado su patron de alimentacion habitual, reduciendo principalmente el consumo de verduras, legumbres y dulces. Se encontro asociacion entre la enteritis radica cronica y el sexo masculino, la edad, la enteritis radica aguda previa y la quimioterapia, pero solo esta resulto asociarse de forma independiente con el desarrollo de enteritis radica cronica despues del analisis multivariante (OR = 3,59 [95% CI 1,20–10,73]). Conclusión: la enteritis cronica es una entidad frecuente en los pacientes tratados con radioterapia pelvica, sobre todo cuando se asocia con quimioterapia. La tasa de complicaciones por esta patologia es baja, pero un numero importante de pacientes realiza modificaciones en su dieta habitual para aliviar o evitar la sintomatologia derivada

  9. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Aqua-FM3_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-10-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  10. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Terra-FM2_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-10-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  11. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Terra-FM1_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-09-30] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  12. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Terra-FM2_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-01-01] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  13. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Terra-FM1_Edition1)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2005-11-01] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  14. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Aqua-FM4_Edition2)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2005-03-29] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  15. Lignes directrices simplifiées sur les lipides

    PubMed Central

    Allan, G. Michael; Lindblad, Adrienne J.; Comeau, Ann; Coppola, John; Hudson, Brianne; Mannarino, Marco; McMinis, Cindy; Padwal, Raj; Schelstraete, Christine; Zarnke, Kelly; Garrison, Scott; Cotton, Candra; Korownyk, Christina; McCormack, James; Nickel, Sharon; Kolber, Michael R.

    2015-01-01

    Résumé Objectif Produire un guide de pratique clinique comportant une approche simplifiée à la prévention primaire des maladies cardiovasculaires (MCV), en insistant sur l’estimation du risque de MCV et la prise en charge des profils lipidiques à l’intention des cliniciens de soins primaires et leurs équipes; nous avons recherché la contribution de professionnels des soins primaires qui n’avaient que peu ou pas de conflits d’intérêts et nous nous sommes concentrés sur les données probantes de la plus haute qualité accessibles. Méthodes Neuf professionnels de la santé (4 médecins de famille, 2 internistes, 1 infirmière praticienne, 1 infirmière autorisée et 1 pharmacienne) et 1 membre non votant (pharmacienne gestionnaire de projet) formaient le comité principal appelé le Lipid Pathway Committee (le comité). La sélection des membres s’est fondée sur la profession, le milieu de pratique et son emplacement. Les membres ont divulgué tous leurs conflits d’intérêts potentiels ou réels. Le processus d’élaboration des lignes directrices était itératif et s’appuyait sur des affichages en ligne, une révision détaillée des données probantes, des réunions par téléphone et en ligne. Le comité a cerné 12 questions prioritaires à répondre. Le groupe de révision des données probantes a répondu à ces questions. À la suite d’un examen des réponses, les principales recommandations ont été formulées par consensus du comité. Nous avons produit une ébauche des lignes directrices qui a ensuite été peaufinée, distribuée à un groupe de cliniciens (médecins de famille, autres spécialistes, pharmaciens, infirmières et infirmières praticiennes) et à des patients pour obtenir de la rétroaction, la réviser en conséquence et le comité l’a ensuite finalisée. Recommandations Des recommandations sont présentées concernant le dépistage et les analyses, les évaluations du risque, le suivi, de même que le r

  16. Atomic Mass and Nuclear Binding Energy for Es-281 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-281 (Einsteinium, atomic number Z = 99, mass number A = 281).

  17. Atomic Mass and Nuclear Binding Energy for Es-322 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-322 (Einsteinium, atomic number Z = 99, mass number A = 322).

  18. Atomic Mass and Nuclear Binding Energy for Es-272 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-272 (Einsteinium, atomic number Z = 99, mass number A = 272).

  19. Atomic Mass and Nuclear Binding Energy for Es-248 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-248 (Einsteinium, atomic number Z = 99, mass number A = 248).

  20. Atomic Mass and Nuclear Binding Energy for Es-241 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-241 (Einsteinium, atomic number Z = 99, mass number A = 241).

  1. Atomic Mass and Nuclear Binding Energy for Es-328 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-328 (Einsteinium, atomic number Z = 99, mass number A = 328).

  2. Atomic Mass and Nuclear Binding Energy for Es-273 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-273 (Einsteinium, atomic number Z = 99, mass number A = 273).

  3. Atomic Mass and Nuclear Binding Energy for Es-289 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-289 (Einsteinium, atomic number Z = 99, mass number A = 289).

  4. Atomic Mass and Nuclear Binding Energy for Es-253 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-253 (Einsteinium, atomic number Z = 99, mass number A = 253).

  5. Atomic Mass and Nuclear Binding Energy for Es-307 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-307 (Einsteinium, atomic number Z = 99, mass number A = 307).

  6. Atomic Mass and Nuclear Binding Energy for Es-312 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-312 (Einsteinium, atomic number Z = 99, mass number A = 312).

  7. Atomic Mass and Nuclear Binding Energy for Es-246 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-246 (Einsteinium, atomic number Z = 99, mass number A = 246).

  8. Atomic Mass and Nuclear Binding Energy for Es-279 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-279 (Einsteinium, atomic number Z = 99, mass number A = 279).

  9. Atomic Mass and Nuclear Binding Energy for Es-303 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-303 (Einsteinium, atomic number Z = 99, mass number A = 303).

  10. Atomic Mass and Nuclear Binding Energy for Es-316 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-316 (Einsteinium, atomic number Z = 99, mass number A = 316).

  11. Atomic Mass and Nuclear Binding Energy for Es-282 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-282 (Einsteinium, atomic number Z = 99, mass number A = 282).

  12. Atomic Mass and Nuclear Binding Energy for Es-284 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-284 (Einsteinium, atomic number Z = 99, mass number A = 284).

  13. Atomic Mass and Nuclear Binding Energy for Es-298 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-298 (Einsteinium, atomic number Z = 99, mass number A = 298).

  14. Atomic Mass and Nuclear Binding Energy for Es-262 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-262 (Einsteinium, atomic number Z = 99, mass number A = 262).

  15. Atomic Mass and Nuclear Binding Energy for Es-254 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-254 (Einsteinium, atomic number Z = 99, mass number A = 254).

  16. Atomic Mass and Nuclear Binding Energy for Es-266 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-266 (Einsteinium, atomic number Z = 99, mass number A = 266).

  17. Atomic Mass and Nuclear Binding Energy for Es-315 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-315 (Einsteinium, atomic number Z = 99, mass number A = 315).

  18. Atomic Mass and Nuclear Binding Energy for Es-286 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-286 (Einsteinium, atomic number Z = 99, mass number A = 286).

  19. Atomic Mass and Nuclear Binding Energy for Es-305 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-305 (Einsteinium, atomic number Z = 99, mass number A = 305).

  20. Atomic Mass and Nuclear Binding Energy for Es-304 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-304 (Einsteinium, atomic number Z = 99, mass number A = 304).

  1. Atomic Mass and Nuclear Binding Energy for Es-278 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-278 (Einsteinium, atomic number Z = 99, mass number A = 278).

  2. Atomic Mass and Nuclear Binding Energy for Es-325 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-325 (Einsteinium, atomic number Z = 99, mass number A = 325).

  3. Atomic Mass and Nuclear Binding Energy for Es-288 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-288 (Einsteinium, atomic number Z = 99, mass number A = 288).

  4. Atomic Mass and Nuclear Binding Energy for Es-314 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-314 (Einsteinium, atomic number Z = 99, mass number A = 314).

  5. Atomic Mass and Nuclear Binding Energy for Es-320 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-320 (Einsteinium, atomic number Z = 99, mass number A = 320).

  6. Atomic Mass and Nuclear Binding Energy for Es-297 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-297 (Einsteinium, atomic number Z = 99, mass number A = 297).

  7. Atomic Mass and Nuclear Binding Energy for Es-267 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-267 (Einsteinium, atomic number Z = 99, mass number A = 267).

  8. Atomic Mass and Nuclear Binding Energy for Es-268 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-268 (Einsteinium, atomic number Z = 99, mass number A = 268).

  9. Atomic Mass and Nuclear Binding Energy for Es-292 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-292 (Einsteinium, atomic number Z = 99, mass number A = 292).

  10. Atomic Mass and Nuclear Binding Energy for Es-274 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-274 (Einsteinium, atomic number Z = 99, mass number A = 274).

  11. Atomic Mass and Nuclear Binding Energy for Es-306 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-306 (Einsteinium, atomic number Z = 99, mass number A = 306).

  12. Atomic Mass and Nuclear Binding Energy for Es-255 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-255 (Einsteinium, atomic number Z = 99, mass number A = 255).

  13. Atomic Mass and Nuclear Binding Energy for Es-287 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-287 (Einsteinium, atomic number Z = 99, mass number A = 287).

  14. Atomic Mass and Nuclear Binding Energy for Es-250 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-250 (Einsteinium, atomic number Z = 99, mass number A = 250).

  15. Atomic Mass and Nuclear Binding Energy for Es-263 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-263 (Einsteinium, atomic number Z = 99, mass number A = 263).

  16. Atomic Mass and Nuclear Binding Energy for Es-285 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-285 (Einsteinium, atomic number Z = 99, mass number A = 285).

  17. Atomic Mass and Nuclear Binding Energy for Es-256 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-256 (Einsteinium, atomic number Z = 99, mass number A = 256).

  18. Atomic Mass and Nuclear Binding Energy for Es-317 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-317 (Einsteinium, atomic number Z = 99, mass number A = 317).

  19. Atomic Mass and Nuclear Binding Energy for Es-269 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-269 (Einsteinium, atomic number Z = 99, mass number A = 269).

  20. Atomic Mass and Nuclear Binding Energy for Es-310 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-310 (Einsteinium, atomic number Z = 99, mass number A = 310).

  1. Atomic Mass and Nuclear Binding Energy for Es-280 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-280 (Einsteinium, atomic number Z = 99, mass number A = 280).

  2. Atomic Mass and Nuclear Binding Energy for Es-291 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-291 (Einsteinium, atomic number Z = 99, mass number A = 291).

  3. Atomic Mass and Nuclear Binding Energy for Es-309 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-309 (Einsteinium, atomic number Z = 99, mass number A = 309).

  4. Atomic Mass and Nuclear Binding Energy for Es-283 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-283 (Einsteinium, atomic number Z = 99, mass number A = 283).

  5. Atomic Mass and Nuclear Binding Energy for Es-243 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-243 (Einsteinium, atomic number Z = 99, mass number A = 243).

  6. Atomic Mass and Nuclear Binding Energy for Es-311 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-311 (Einsteinium, atomic number Z = 99, mass number A = 311).

  7. Atomic Mass and Nuclear Binding Energy for Es-275 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-275 (Einsteinium, atomic number Z = 99, mass number A = 275).

  8. Atomic Mass and Nuclear Binding Energy for Es-265 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-265 (Einsteinium, atomic number Z = 99, mass number A = 265).

  9. Atomic Mass and Nuclear Binding Energy for Es-247 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-247 (Einsteinium, atomic number Z = 99, mass number A = 247).

  10. Atomic Mass and Nuclear Binding Energy for Es-276 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-276 (Einsteinium, atomic number Z = 99, mass number A = 276).

  11. Atomic Mass and Nuclear Binding Energy for Es-321 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-321 (Einsteinium, atomic number Z = 99, mass number A = 321).

  12. Atomic Mass and Nuclear Binding Energy for Es-295 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-295 (Einsteinium, atomic number Z = 99, mass number A = 295).

  13. Atomic Mass and Nuclear Binding Energy for Es-271 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-271 (Einsteinium, atomic number Z = 99, mass number A = 271).

  14. Atomic Mass and Nuclear Binding Energy for Es-313 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-313 (Einsteinium, atomic number Z = 99, mass number A = 313).

  15. Atomic Mass and Nuclear Binding Energy for Es-308 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-308 (Einsteinium, atomic number Z = 99, mass number A = 308).

  16. Atomic Mass and Nuclear Binding Energy for Es-323 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-323 (Einsteinium, atomic number Z = 99, mass number A = 323).

  17. Atomic Mass and Nuclear Binding Energy for Es-329 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-329 (Einsteinium, atomic number Z = 99, mass number A = 329).

  18. Atomic Mass and Nuclear Binding Energy for Es-327 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-327 (Einsteinium, atomic number Z = 99, mass number A = 327).

  19. Atomic Mass and Nuclear Binding Energy for Es-296 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-296 (Einsteinium, atomic number Z = 99, mass number A = 296).

  20. Atomic Mass and Nuclear Binding Energy for Es-302 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-302 (Einsteinium, atomic number Z = 99, mass number A = 302).

  1. Atomic Mass and Nuclear Binding Energy for Es-324 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-324 (Einsteinium, atomic number Z = 99, mass number A = 324).

  2. Atomic Mass and Nuclear Binding Energy for Es-260 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-260 (Einsteinium, atomic number Z = 99, mass number A = 260).

  3. Atomic Mass and Nuclear Binding Energy for Es-259 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-259 (Einsteinium, atomic number Z = 99, mass number A = 259).

  4. Atomic Mass and Nuclear Binding Energy for Es-244 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-244 (Einsteinium, atomic number Z = 99, mass number A = 244).

  5. Atomic Mass and Nuclear Binding Energy for Es-242 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-242 (Einsteinium, atomic number Z = 99, mass number A = 242).

  6. Atomic Mass and Nuclear Binding Energy for Es-270 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-270 (Einsteinium, atomic number Z = 99, mass number A = 270).

  7. Atomic Mass and Nuclear Binding Energy for Es-251 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-251 (Einsteinium, atomic number Z = 99, mass number A = 251).

  8. Atomic Mass and Nuclear Binding Energy for Es-299 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-299 (Einsteinium, atomic number Z = 99, mass number A = 299).

  9. Atomic Mass and Nuclear Binding Energy for Es-252 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-252 (Einsteinium, atomic number Z = 99, mass number A = 252).

  10. Atomic Mass and Nuclear Binding Energy for Es-290 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-290 (Einsteinium, atomic number Z = 99, mass number A = 290).

  11. Atomic Mass and Nuclear Binding Energy for Es-293 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-293 (Einsteinium, atomic number Z = 99, mass number A = 293).

  12. Atomic Mass and Nuclear Binding Energy for Es-330 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-330 (Einsteinium, atomic number Z = 99, mass number A = 330).

  13. Atomic Mass and Nuclear Binding Energy for Es-300 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-300 (Einsteinium, atomic number Z = 99, mass number A = 300).

  14. Atomic Mass and Nuclear Binding Energy for Es-326 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-326 (Einsteinium, atomic number Z = 99, mass number A = 326).

  15. Atomic Mass and Nuclear Binding Energy for Es-245 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-245 (Einsteinium, atomic number Z = 99, mass number A = 245).

  16. Atomic Mass and Nuclear Binding Energy for Es-249 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-249 (Einsteinium, atomic number Z = 99, mass number A = 249).

  17. Atomic Mass and Nuclear Binding Energy for Es-264 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-264 (Einsteinium, atomic number Z = 99, mass number A = 264).

  18. Atomic Mass and Nuclear Binding Energy for Es-301 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-301 (Einsteinium, atomic number Z = 99, mass number A = 301).

  19. Atomic Mass and Nuclear Binding Energy for Es-294 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-294 (Einsteinium, atomic number Z = 99, mass number A = 294).

  20. Atomic Mass and Nuclear Binding Energy for Es-258 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-258 (Einsteinium, atomic number Z = 99, mass number A = 258).

  1. Atomic Mass and Nuclear Binding Energy for Es-319 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-319 (Einsteinium, atomic number Z = 99, mass number A = 319).

  2. Atomic Mass and Nuclear Binding Energy for Es-261 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-261 (Einsteinium, atomic number Z = 99, mass number A = 261).

  3. Atomic Mass and Nuclear Binding Energy for Es-331 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-331 (Einsteinium, atomic number Z = 99, mass number A = 331).

  4. Atomic Mass and Nuclear Binding Energy for Es-332 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-332 (Einsteinium, atomic number Z = 99, mass number A = 332).

  5. Atomic Mass and Nuclear Binding Energy for Es-318 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-318 (Einsteinium, atomic number Z = 99, mass number A = 318).

  6. Atomic Mass and Nuclear Binding Energy for Es-277 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-277 (Einsteinium, atomic number Z = 99, mass number A = 277).

  7. Atomic Mass and Nuclear Binding Energy for Es-257 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-257 (Einsteinium, atomic number Z = 99, mass number A = 257).

  8. Maladies reliées aux loisirs aquatiques

    PubMed Central

    Sanborn, Margaret; Takaro, Tim

    2013-01-01

    Résumé Objectif Passer en revue les facteurs de risque, la prise en charge et la prévention des maladies reliées aux loisirs aquatiques en pratique familiale. Sources des données Des articles originaux et de synthèse entre janvier 1998 et février 2012 ont été identifiés à l’aide de PubMed et des expressions de recherche en anglais water-related illness, recreational water illness et swimmer illness. Message principal Il y a un risque de 3 % à 8 % de maladies gastrointestinales (MGI) après la baignade. Les groupes à risque élevé de MGI sont les enfants de moins de 5 ans, surtout s’ils n’ont pas été vaccinés contre le rotavirus, les personnes âgées et les patients immunodéficients. Les enfants sont à plus grand risque parce qu’ils avalent plus d’eau quand ils nagent, restent dans l’eau plus longtemps et jouent dans l’eau peu profonde et le sable qui sont plus contaminés. Les adeptes des sports dans lesquels le contact avec l’eau est abondant comme le triathlon et le surf cerf-volant sont aussi à risque élevé et même ceux qui s’adonnent à des activités impliquant un contact partiel avec l’eau comme la navigation de plaisance et la pêche ont un risque de 40 % à 50 % fois plus grand de MGI par rapport à ceux qui ne pratiquent pas de sports aquatiques. Il y a lieu de faire une culture des selles quand on soupçonne une maladie reliée aux loisirs aquatiques et l’échelle clinique de la déshydratation est utile pour l’évaluation des besoins de traitement chez les enfants affectés. Conclusion Les maladies reliées aux loisirs aquatiques est la principale cause de MGI durant la saison des baignades. La reconnaissance que la baignade est une source importante de maladies peut aider à prévenir les cas récurrents et secondaires. On recommande fortement le vaccin contre le rotavirus chez les enfants qui se baignent souvent.

  9. GroES in the asymmetric GroEL14–GroES7 complex exchanges via an associative mechanism

    PubMed Central

    Horowitz, Paul M.; Lorimer, George H.; Ybarra, Jesse

    1999-01-01

    The interaction of the chaperonin GroEL14 with its cochaperonin GroES7 is dynamic, involving stable, asymmetric 1:1 complexes (GroES7⋅GroEL7–GroEL7) and transient, metastable symmetric 2:1 complexes [GroES7⋅GroEL7–GroEL7⋅GroES7]. The transient formation of a 2:1 complex permits exchange of free GroES7 for GroES7 bound in the stable 1:1 complex. Electrophoresis in the presence of ADP was used to resolve free GroEL14 from the GroES7–GroEL14 complex. Titration of GroEL14 with radiolabeled GroES7 to molar ratios of 32:1 demonstrated a 1:1 limiting stoichiometry in a stable complex. No stable 2:1 complex was detected. Preincubation of the asymmetric GroES7⋅GroEL7–GroEL7 complex with excess unlabeled GroES7 in the presence of ADP demonstrated GroES7 exchange. The rates of GroES7 exchange were proportional to the concentration of unlabeled free GroES7. This concentration dependence points to an associative mechanism in which exchange of GroES7 occurs by way of a transient 2:1 complex and excludes a dissociative mechanism in which exchange occurs by way of free GroEL14. Exchange of radiolabeled ADP from 1:1 complexes was much slower than the exchange of GroES7. In agreement with recent structural studies, this indicates that conformational changes in GroEL14 following the dissociation of GroES7 must precede ADP release. These results explain how the GroEL14 cavity can become reversibly accessible to proteins under in vivo conditions that favor 2:1 complexes. PMID:10077571

  10. ES&H development activities for the W89 warhead

    SciTech Connect

    Pretzel, C.W.

    1995-08-01

    Environmental, Safety and Health (ES&H) issues became an important design consideration during the development of the W89 warhead for the SRAM 11 (Short-Range Attack Missile) missile. An action plan was developed to handle these issues at all the production agencies and at both the system and the component level. The main thrust was in the area of solvent substitution, in particular for solder flux removal. The cleaner d-limonene followed by an isopropyl alcohol rinse was selected for applications were the traditional cleaners were 1,1,1 trichloroethane or trichloroethylene. Compatibility testing rather than efficacy testing dominated the development effort. In addition to other solvent substitution applications, organic materials that were free of toluene diisocynate (TDI), and methylene dianiline (MDA) were explored for use in the W89.

  11. es11_Rappe_final_report_DOE.pdf

    SciTech Connect

    Rappe, Andrew M

    2011-08-31

    ES11: the 23rd Annual Workshop on Electronic Structure Methods was held from June 6-9, 2011 at the University of Pennsylvania. The local organizing committee (see Section II) led by PI Andrew M. Rappe supervised the organization of the conference, before, during, and after the meeting itself. The national organizing committee set the technical program of talks, and provided support and advice in various ways. The conference was well-attended (see Section III). An important feature of this conference was a series of panel discussions (see Section IV) to discuss the field of electronic structure and to set new directions. The technical program was of extraordinarily high quality (see Section V). The host institution, the University of Pennsylvania, provided a supportive environment for this meeting (see Section VI).

  12. Urban agriculture and Anopheles habitats in Dar es Salaam, Tanzania.

    PubMed

    Dongus, Stefan; Nyika, Dickson; Kannady, Khadija; Mtasiwa, Deo; Mshinda, Hassan; Gosoniu, Laura; Drescher, Axel W; Fillinger, Ulrike; Tanner, Marcel; Killeen, Gerry F; Castro, Marcia C

    2009-05-01

    A cross-sectional survey of agricultural areas, combined with routinely monitored mosquito larval information, was conducted in urban Dar es Salaam, Tanzania, to investigate how agricultural and geographical features may influence the presence of Anopheles larvae. Data were integrated into a geographical information systems framework, and predictors of the presence of Anopheles larvae in farming areas were assessed using multivariate logistic regression with independent random effects. It was found that more than 5% of the study area (total size 16.8 km2) was used for farming in backyard gardens and larger open spaces. The proportion of habitats containing Anopheles larvae was 1.7 times higher in agricultural areas compared to other areas (95% confidence interval = 1.56-1.92). Significant geographic predictors of the presence of Anopheles larvae in gardens included location in lowland areas, proximity to river, and relatively impermeable soils. Agriculture-related predictors comprised specific seedbed types, mid-sized gardens, irrigation by wells, as well as cultivation of sugar cane or leafy vegetables. Negative predictors included small garden size, irrigation by tap water, rainfed production and cultivation of leguminous crops or fruit trees. Although there was an increased chance of finding Anopheles larvae in agricultural sites, it was found that breeding sites originated by urban agriculture account for less than a fifth of all breeding sites of malaria vectors in Dar es Salaam. It is suggested that strategies comprising an integrated malaria control effort in malaria-endemic African cities include participatory involvement of farmers by planting shade trees near larval habitats.

  13. 75 FR 51876 - Proposed Collection; Comment Request for Form 1041-ES

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-23

    ... Internal Revenue Service Proposed Collection; Comment Request for Form 1041-ES AGENCY: Internal Revenue...(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 1041-ES, Estimated Income Tax...: Estimated Income Tax for Estates and Trusts. OMB Number: 1545-0971. ] Form Number: Form 1041-ES....

  14. 78 FR 58607 - Proposed Collection; Comment Request for Form 1041-ES

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-24

    ... Internal Revenue Service Proposed Collection; Comment Request for Form 1041-ES AGENCY: Internal Revenue...(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 1041-ES, Estimated Income Tax... Income Tax for Estates and Trusts. OMB Number: 1545-0971. Form Number: Form 1041-ES. Abstract:...

  15. MicroRNA Expression Characterizes Oligometastasis(es)

    PubMed Central

    Lussier, Yves A.; Darga, Thomas E.; Malik, Renuka; Fan, Hanli; Perakis, Samantha; Filippo, Matthew; Corbin, Kimberly; Lee, Younghee; Posner, Mitchell C.; Chmura, Steven J.; Hellman, Samuel; Weichselbaum, Ralph R.

    2011-01-01

    Background Cancer staging and treatment presumes a division into localized or metastatic disease. We proposed an intermediate state defined by ≤5 cumulative metastasis(es), termed oligometastases. In contrast to widespread polymetastases, oligometastatic patients may benefit from metastasis-directed local treatments. However, many patients who initially present with oligometastases progress to polymetastases. Predictors of progression could improve patient selection for metastasis-directed therapy. Methods Here, we identified patterns of microRNA expression of tumor samples from oligometastatic patients treated with high-dose radiotherapy. Results Patients who failed to develop polymetastases are characterized by unique prioritized features of a microRNA classifier that includes the microRNA-200 family. We created an oligometastatic-polymetastatic xenograft model in which the patient-derived microRNAs discriminated between the two metastatic outcomes. MicroRNA-200c enhancement in an oligometastatic cell line resulted in polymetastatic progression. Conclusions These results demonstrate a biological basis for oligometastases and a potential for using microRNA expression to identify patients most likely to remain oligometastatic after metastasis-directed treatment. PMID:22174856

  16. Capteurs à ondes élastiques guidées

    NASA Astrophysics Data System (ADS)

    Royer, D.; Dieulesaint, E.; Legras, O.

    1992-01-01

    The sensors described here have been studied in our laboratory. The industrial development of some of them is now in progress. They exploit the properties of elastic waves propagating, according to a chosen mode, in a plate or in a cylinder. The sensors comprising a plate are a graphic tablet, a map coordinate resolver, a touch screen and a liquid presence detector. The sensors comprising a cylinder are a liquid presence detector and a liquid level sensor. Before describing these devices, we comment on the dispersion curves and express the attenuation factor for guided waves in presence of a liquid. Les capteurs présentés dont certains sont en cours de développement industriel ont été étudiés dans notre laboratoire. Ils exploitent les propriétés d'ondes élastiques se propageant, suivant un mode choisi, dans une plaque ou un cylindre. Les capteurs comprenant une plaque sont une tablette de saisie de graphiques, un coordinomètre, un écran tactile et un détecteur de la présence d'un liquide, les capteurs comprenant un cylindre ou un tube sont un détecteur de présence et un jaugeur du niveau d'un liquide. Avant de les décrire, nous commentons les courbes de dispersion et calculons le coefficient d'atténuation de ces ondes guidées en présence d'un liquide.

  17. Homicide of children in Dar es Salaam, Tanzania, 2005

    PubMed Central

    Outwater, Anne; Mgaya, Edward; Campbell, Jacquelyn C.; Becker, Stan; Kinabo, Linna; Menick, Daniel Mbassa

    2014-01-01

    Background Although data are sparse, it has been estimated that the highest rates of homicide death amongst children are in Africa. Little information is available on ages 0 -< 15 years. No reliable quantitative surveillance analysis of neonaticide (killed at less than one week) has been done. Methods A Violent Death Survey following WHO/CDC Guidelines was completed in Dar es Salaam region, Tanzania (DSM) (population 2.845 million) in 2005. Qualitative and quantitative data were gathered and analyzed using mixed methods techniques. Results The overall age adjusted rate of discarded and killed children in DSM was 2.05. The rate of neonaticide was 27.7 per 100,000) while the rate of homicide incidence for children > one day was Discussion The overall estimated homicide rate for Africa of children under age 15 was 4.53 per 100,000, whereas. The estimated global rate is 1.7 per 100,000 closer to DSM‘s rate. The results in DSM show that broad age groupings such as ” <1 year” or “0–4 years” or “0 – <15 years” may mask a high incidence of neonaticide and an otherwise low incidence of murdered children. The print media provided good in-depth coverage for a few cases but it is not known if the reported cases are representative. Conclusion Eighty percent of homicides of children in DSM are neonaticides. Since it is believed that the forces behind neonaticide are fundamentally different than homicides of older children, it is suggested that data of future surveys be parsed to include neonates, until the phenomenon is more clearly understood and addressed. Further understanding of the mother and father of the deceased is needed. Continued surveillance data collection is important to expand the sample size. PMID:22066333

  18. ES and H-compatible lubrication for duplex bearings

    SciTech Connect

    Steinhoff, R.G.

    1997-10-01

    Two ES and H-compatible lubricants (environment, safety, and health) for duplex bearing applications and one hybrid material duplex bearing were evaluated and compared against duplex bearings with trichlorotrifluoroethane (Freon) deposition of low molecular weight polytetrafluoroethylene (PTFE) bearing lubricant extracted from Vydax{trademark}. Vydax is a product manufactured by DuPont consisting of various molecular weights of PTFE suspended in trichlorotrifluoroethane (Freon), which is an ozone-depleting solvent. Vydax has been used as a bearing lubricant in strong link mechanisms since 1974. Hybrid duplex bearings with silicon nitride balls and molded glass-nylon-Teflon retainers, duplex bearings lubricated with sputtered MoS{sub 2} on races and retainers, and duplex bearings lubricated with electrophoretic deposited MoS{sub 2} were evaluated. Bearings with electrophoretic deposited MoS{sub 2} performed as well as bearings with Freon deposition of PTFE from Freon-based Vydax. Hybrid bearings with silicon nitride balls performed worse than bearings lubricated with Vydax, but their performance would still be acceptable for most applications. Bearings lubricated with sputtered MoS{sub 2} on the races and retainers had varying amounts of film on the bearings. This affected the performance of the bearings. Bearings with a uniform coating performed to acceptable levels, but bearings with no visible MoS{sub 2} on the races and retainers did not perform as well as bearings with the other coatings. Unless process controls are incorporated in the sputtering process or the bearings are screened, they do not appear to be acceptable for duplex bearing applications.

  19. Generation of Hprt-disrupted rat through mouse←rat ES chimeras

    PubMed Central

    Isotani, Ayako; Yamagata, Kazuo; Okabe, Masaru; Ikawa, Masahito

    2016-01-01

    We established rat embryonic stem (ES) cell lines from a double transgenic rat line which harbours CAG-GFP for ubiquitous expression of GFP in somatic cells and Acr3-EGFP for expression in sperm (green body and green sperm: GBGS rat). By injecting the GBGS rat ES cells into mouse blastocysts and transplanting them into pseudopregnant mice, rat spermatozoa were produced in mouse←rat ES chimeras. Rat spermatozoa from the chimeric testis were able to fertilize eggs by testicular sperm extraction combined with intracytoplasmic sperm injection (TESE-ICSI). In the present paper, we disrupted rat hypoxanthine-guanine phosphoribosyl transferase (Hprt) gene in ES cells and produced a Hprt-disrupted rat line using the mouse←rat ES chimera system. The mouse←rat ES chimera system demonstrated the dual advantages of space conservation and a clear indication of germ line transmission in knockout rat production. PMID:27062982

  20. Base de données des étoiles doubles et multiples.

    NASA Astrophysics Data System (ADS)

    Oblak, E.; Chareton, M.; Dumon, R.; Kurpinska-Winiarska, M.; Kundera, T.

    Les auteurs réalisent à Besançon une base de données de tous les types d'étoiles doubles et multiples. Le traitement des données concerne l'acquisition de nouvelles données, l'examen de celles déjà disponibles, et le développement d'un outil informatique de gestion et d'interrogation à distance de la base.

  1. Hyperfine field of einsteinium in iron and nuclear magnetic moment of Es254

    NASA Astrophysics Data System (ADS)

    Severijns, N.; Belyaev, A. A.; Erzinkyan, A. L.; Eversheim, P.-D.; Filimonov, V. T.; Golovko, V. V.; Gurevich, G. M.; Herzog, P.; Kraev, I. S.; Lukhanin, A. A.; Noga, V. I.; Parfenova, V. P.; Phalet, T.; Rusakov, A. V.; Tandecki, M.; Toporov, Yu. G.; Tramm, C.; Traykov, E.; Gorp, S. Van; Vyachin, V. N.; Wauters, F.; Zákoucký, D.; Zotov, E.

    2009-06-01

    The angular distributions of γ rays and α particles from oriented Bk250, Es253,254, and Fm255 nuclei were investigated to extract hyperfine interaction information for these actinide impurities in an iron host lattice. The hyperfine field of einsteinium in iron was found to be |Bhf(EsFe̲|)=396(32) T. With this value the magnetic moment of Es254 was then determined as |μ|=4.35(41)μN.

  2. Interpretation of ES, CS, and IOS approximations within a translational-internal coupling scheme. IV. ES and IOS molecule-molecule cross sections

    NASA Astrophysics Data System (ADS)

    Snider, R. F.; Parvatiyar, M. G.

    1981-05-01

    Properties of energy sudden and infinite order sudden translational-internal reduced S matrices are given for general molecule-molecule collisions. Formal similarities with the distorted wave Born approximation are discussed. Structural simplifications of energy dependent and kinetic cross sections associated with making the ES approximation are described. Conceptual difficulties associated with applying the ES and IOS approximations to kinetic processes dominated by energetically inelastic collisions are pointed out.

  3. Low-latitude Es capable of controlling the onset of equatorial spread F

    NASA Astrophysics Data System (ADS)

    Joshi, L. M.; Patra, A. K.; Rao, S. V. B.

    2013-03-01

    investigate their role on the occurrence of equatorial spread F (ESF), the low-latitude E region in the evening hours has been characterized in terms of sporadic E (Es) activity based on ionosonde observations from Sriharikota, a low-latitude station in India. While prior to the sunset, either Chapman type E layer, or Es, or a combination of the two was observed, after sunset, when observed, the E layer was exclusively Es. Es, however, could be either blanketing or nonblanketing type. Observations revealed that while the occurrence of ESF had a broad relationship with the type of Es, a detailed investigation suggested that (1) ESF occurred (did not occur) irrespective of Es type if the virtual height of the F layer base (h'F) was greater than ~360 km (less than ~290 km), and (2) ESF occurrence depended strongly on the type of Es if 290 km < h'F < 360 km. Model computation shows that low-latitude Es can significantly influence the growth of the Rayleigh-Taylor instability for the cases corresponding to category 2 by increasing the total field-line integrated Pedersen conductivity, consistent with the observations. The results presented in this paper highlight under what circumstances the low-latitude Es would play a critical role in the occurrence of ESF, which can be used to understand the day-to-day variability of ESF.

  4. Comparing the accuracy of ES-BC, EIS-GS, and ES Oxi on body composition, autonomic nervous system activity, and cardiac output to standardized assessments

    PubMed Central

    Lewis, John E; Tannenbaum, Stacey L; Gao, Jinrun; Melillo, Angelica B; Long, Evan G; Alonso, Yaima; Konefal, Janet; Woolger, Judi M; Leonard, Susanna; Singh, Prabjot K; Chen, Lawrence; Tiozzo, Eduard

    2011-01-01

    Background and purpose The Electro Sensor Complex (ESC) is software that combines three devices using bioelectrical impedance, galvanic skin response, and spectrophotometry: (1) ES-BC (Electro Sensor-Body Composition; LD Technology, Miami, FL) to assess body composition, (2) EIS-GS (Electro Interstitial Scan-Galvanic Skin; LD Technology) to predict autonomic nervous system activity, and (3) ES Oxi (Electro Sensor Oxi; LD Technology) to assess cardiac output. The objective of this study was to compare each to a standardized assessment: ES-BC to dual-energy X-ray absorptiometry (DXA), EIS-GS to heart rate variability, and ES Oxi to BioZ Dx Diagnostic System (BioZ Dx; SonoSite Inc, Bothell, WA). Patients and methods The study was conducted in two waves. Fifty subjects were assessed for body composition and autonomic nervous system activity. Fifty-one subjects were assessed for cardiac output. Results We found adequate relative and absolute agreement between ES-BC and DXA for fat mass (r = 0.97, P < 0.001) with ES-BC overestimating fat mass by 0.1 kg and for body fat percentage (r = 0.92, P < 0.001) with overestimation of fat percentage by 0.4%. For autonomic nervous system activity, we found marginal relative agreement between EIS-GS and heart rate variability by using EIS-GS as the predictor in a linear regression equation (adjusted R2 = 0.56, P = 0.03). For cardiac output, adequate relative and absolute agreement was found between ES Oxi and BioZ Dx at baseline (r = 0.60, P < 0.001), after the first exercise stage (r = 0.79, P < 0.001), and after the second exercise stage (r = 0.86, P < 0.001). Absolute agreement was found at baseline and after both bouts of exercise; ES Oxi overestimated baseline and stage 1 exercise cardiac output by 0.3 L/minute and 0.1 L/minute, respectively, but exactly estimated stage 2 exercise cardiac output. Conclusion ES-BC and ES Oxi accurately assessed body composition and cardiac output compared to standardized instruments, whereas EIS

  5. The second anti-lipopolysaccharide factor (EsALF-2) with antimicrobial activity from Eriocheir sinensis.

    PubMed

    Zhang, Ying; Wang, Lingling; Wang, Leilei; Yang, Jialong; Gai, Yunchao; Qiu, Limei; Song, Linsheng

    2010-09-01

    The anti-lipopolysaccharide factor (ALF) is a small basic protein that can bind and neutralize lipopolysaccharide (LPS), mediating degranulation and activation of an intracellular coagulation cascade. In the present study, cDNA of the second Eriocheir sinensis ALF (designated as EsALF-2) was cloned and the full-length cDNA of EsALF-2 was of 724bp, consisting of an open reading frame (ORF) of 363bp encoding a polypeptide of 120 amino acids. The deduced amino acid of EsALF-2 shared 82% similarity with EsALF-1 from E. sinensis and about 53-65% similarity with ALFs from other crustaceans. The potential tertiary structures of EsALF-1 and EsALF-2 contained two highly conserved-cysteine residues to define the LPS binding site, but the N-terminal of EsALF-1 formed a single additional alpha-helix compared to EsALF-2, implying that EsALF-1 and EsALF-2 might represent different biological functions in E. sinensis. The mRNA transcript of EsALF-2 was detected in all examined tissues of healthy crabs, including haemocytes, hepatopancreas, gill, muscle, heart and gonad, which suggested that EsALF-2 could be a multifunctional molecule for the host immune defense responses and thereby provided systemic protection against pathogens. The mRNA expression of EsALF-2 was up-regulated after Listonella anguillarum and Pichia pastoris challenge and the recombinant protein of EsALF-2 showed antimicrobial activity against L. anguillarum and P. pastoris, indicating that EsALF-2 was involved in the immune defense responses in Chinese mitten crab against L. anguillarum and P. pastoris. These results together indicated that there were abundant and diverse ALFs in E. sinensis with various biological functions and these ALFs would provide candidate promising therapeutic or prophylactic agents in health management and diseases control of crab aquaculture. PMID:20416335

  6. Daytime dependence of disturbances of ionospheric Es-layers connected to earthquakes

    NASA Astrophysics Data System (ADS)

    Liperovskaya, E. V.; Liperovsky, A. V.; Meister, C.-V.; Silina, A. S.

    2012-04-01

    In the present work variations of the semi-transparency of the sporadic E-layer of the ionosphere due to seismic activities are studied. The semi-transparency Q is determined by the blanketing frequency fbEs and the characteristic frequency foEs, Q = (foEs - fbEs)/fbEs. At low values of the blanketing frequency fbEs, the critical frequency foEs does not describe the maximum ionisation density of the Es-layer, as the critical frequencies of regular ionospheric layers (e.g. foF2) do, but it describes the occurrence of small-scall (tenths of meters) inhomogeneities of the ionisation density along the vertical in the layer. The maximum ionisation density of the sporadic layer is proportional to the square of fbEs. In the case of vertical ionospheric sounding, the sporadic layer becomes transparent for signals with frequencies larger than fbEs. Investigations showed that about three days before an earthquake an increase of the semi-transparency interval is observed during sunset and sunrise. In the present work, analogous results are found for data of the vertical sounding stations "Tokyo" and "Petropavlovsk-Kamchatsky". Using the method of superposition of epoches, more than 50 earthquakes with magnitudes M > 5, depths h < 40 km, and distances between the station and the epicenter R < 300 km are considered in case of the vertical sounding station "Tokyo". More than 20 earthquakes with such parameters were analysed in case of the station "Petropavlovsk-Kamchatsky". Days with strong geomagnetic activity were excluded from the analysis. According to the station "Petropavlovsk-Kamchatsky" about 1-3 days before earthquakes, an increase of Es-spread is observed a few hours before midnight. This increase is a sign of large-scale inhomogeneities in the sporadic layers.

  7. Functional characterization of an archaeal GroEL/GroES chaperonin system: significance of substrate encapsulation.

    PubMed

    Figueiredo, Luis; Klunker, Daniel; Ang, Debbie; Naylor, Dean J; Kerner, Michael J; Georgopoulos, Costa; Hartl, F Ulrich; Hayer-Hartl, Manajit

    2004-01-01

    In all three kingdoms of life chaperonins assist the folding of a range of newly synthesized proteins. As shown recently, Archaea of the genus Methanosarcina contain both group I (GroEL/GroES) and group II (thermosome) chaperonins in the cytosol. Here we report on a detailed functional analysis of the archaeal GroEL/GroES system of Methanosarcina mazei (Mm) in comparison to its bacterial counterpart from Escherichia coli (Ec). We find that the groESgroEL operon of M. mazei is unable to functionally replace groESgroEL in E. coli. However, the MmGroES protein can largely complement a mutant EcGroES protein in vivo. The ATPase rate of MmGroEL is very low and the dissociation of MmGroES from MmGroEL is 15 times slower than for the EcGroEL/GroES system. This slow ATPase cycle results in a prolonged enclosure time for model substrate proteins, such as rhodanese, in the MmGroEL:GroES folding cage before their release into the medium. Interestingly, optimal functionality of MmGroEL/GroES and its ability to encapsulate larger proteins, such as malate dehydrogenase, requires the presence of ammonium sulfate in vitro. In the absence of ammonium sulfate, malate dehydrogenase fails to be encapsulated by GroES and rather cycles on and off the GroEL trans ring in a non-productive reaction. These results indicate that the archaeal GroEL/GroES system has preserved the basic encapsulation mechanism of bacterial GroEL and suggest that it has adjusted the length of its reaction cycle to the slower growth rates of Archaea. Additionally, the release of only the folded protein from the GroEL/GroES cage may prevent adverse interactions of the GroEL substrates with the thermosome, which is not normally located within the same compartment. PMID:14576149

  8. Accumulation of /sup 254g/Es in the neutron irradiation of californium

    SciTech Connect

    Adaev, V.A.; Klinov, A.V.; Mamelin, A.V.; Toporov, Yu.G.

    1987-06-01

    Preliminary calculations have shown that the ratio of the rates of formation and burnup of /sup 254g/Es can be shifted toward the rate of formation, provided that the californium-containing targets are irradiated with neutrons having a very hard spectrum, (the hardness of the neutron spectrum is defined as the ratio of the flux density of ipithermal neutrons per unit mean logarithmic energy decrement to the flux density of the thermal neutrons). For the purpose of experimentally verifying this conclusion, we irradiated two test samples provided with metal cadmium shields and containing a mixture of californium isotopes as the initial material (approx. 70% of the total was /sup 252/Cf nuclei). After target irradiation, the targets were subjected to a radiochemical treatment and the concentration of the products accumulated was analyzed. It follows from a comparison of experimental and calculated data that the published data on the neutron cross section of the einsteinium isotopes render excessive /sup 254g/Es yields and too small /sup 253/Es yields. Therefore a series of calculations was made for the various values of the resonance integral of the /sup 253/Es absorption and of the partial resonances of the radiative capture integrals of /sup 253/Es in the reaction leading to the formation of /sup 254g/Es and /sup 254m/Es. The calculations have shown that the /sup 253/Es yield in the targets can be satisfactorily predicted with a resonance integral I/sub a/ = 3500 b of absorption

  9. An in situ hybridization-based screen for heterogeneously expressed genes in mouse ES cells.

    PubMed

    Carter, Mark G; Stagg, Carole A; Falco, Geppino; Yoshikawa, Toshiyuki; Bassey, Uwem C; Aiba, Kazuhiro; Sharova, Lioudmila V; Shaik, Nabeebi; Ko, Minoru S H

    2008-02-01

    We previously reported that Zscan4 showed heterogeneous expression patterns in mouse embryonic stem (ES) cells. To identify genes that show similar expression patterns, we carried out high-throughput in situ hybridization assays on ES cell cultures for 244 genes. Most of the genes are involved in transcriptional regulation, and were selected using microarray-based comparisons of gene expression profiles in ES and embryonal carcinoma (EC) cells versus differentiated cell types. Pou5f1 (Oct4, Oct3/4) and Krt8 (EndoA) were used as controls. Hybridization signals were detected on ES cell colonies for 147 genes (60%). The majority (136 genes) of them showed relatively homogeneous expression in ES cell colonies. However, we found that two genes unequivocally showed Zscan4-like spotted expression pattern (spot-in-colony pattern; Whsc2 and Rhox9). We also found that nine genes showed relatively heterogeneous expression pattern (mosaic-in-colony pattern: Zfp42/Rex1, Rest, Atf4, Pa2g4, E2f2, Nanog, Dppa3/Pgc7/Stella, Esrrb, and Fscn1). Among these genes, Zfp42/Rex1 showed unequivocally heterogeneous expression in individual ES cells prepared by the CytoSpin. These results show the presence of different types or states of cells within ES cell cultures otherwise thought to be undifferentiated and homogeneous, suggesting a previously unappreciated complexity in ES cell cultures.

  10. ES1 is a mitochondrial enlarging factor contributing to form mega-mitochondria in zebrafish cones.

    PubMed

    Masuda, Takamasa; Wada, Yasutaka; Kawamura, Satoru

    2016-03-01

    Total mass of mitochondria increases during cell proliferation and differentiation through mitochondrial biogenesis, which includes mitochondrial proliferation and growth. During the mitochondrial growth, individual mitochondria have been considered to be enlarged independently of mitochondrial fusion. However, molecular basis for this enlarging process has been poorly understood. Cone photoreceptor cells in the retina possess large mitochondria, so-called mega-mitochondria that have been considered to arise via the enlarging process. Here we show that ES1 is a novel mitochondria-enlarging factor contributing to form mega-mitochondria in cones. ES1 is specifically expressed in cones and localized to mitochondria including mega-mitochondria. Knockdown of ES1 markedly reduced the mitochondrial size in cones. In contrast, ectopic expression of ES1 in rods significantly increased both the size of individual mitochondria and the total mass of the mitochondrial cluster without changing the number of them. RNA-seq analysis showed that ERRα and its downstream mitochondrial genes were significantly up-regulated in the ES1-expressing rods, suggesting facilitation of mitochondrial enlargement via ERRα-dependent processes. Furthermore, higher energy state was detected in the ES1-expressing rods, indicating that the enlarged mitochondria by ES1 are capable of producing high energy. ES1 is the mitochondrial protein that is first found to promote enlargement of individual mitochondria.

  11. ES1 is a mitochondrial enlarging factor contributing to form mega-mitochondria in zebrafish cones

    PubMed Central

    Masuda, Takamasa; Wada, Yasutaka; Kawamura, Satoru

    2016-01-01

    Total mass of mitochondria increases during cell proliferation and differentiation through mitochondrial biogenesis, which includes mitochondrial proliferation and growth. During the mitochondrial growth, individual mitochondria have been considered to be enlarged independently of mitochondrial fusion. However, molecular basis for this enlarging process has been poorly understood. Cone photoreceptor cells in the retina possess large mitochondria, so-called mega-mitochondria that have been considered to arise via the enlarging process. Here we show that ES1 is a novel mitochondria-enlarging factor contributing to form mega-mitochondria in cones. ES1 is specifically expressed in cones and localized to mitochondria including mega-mitochondria. Knockdown of ES1 markedly reduced the mitochondrial size in cones. In contrast, ectopic expression of ES1 in rods significantly increased both the size of individual mitochondria and the total mass of the mitochondrial cluster without changing the number of them. RNA-seq analysis showed that ERRα and its downstream mitochondrial genes were significantly up-regulated in the ES1-expressing rods, suggesting facilitation of mitochondrial enlargement via ERRα-dependent processes. Furthermore, higher energy state was detected in the ES1-expressing rods, indicating that the enlarged mitochondria by ES1 are capable of producing high energy. ES1 is the mitochondrial protein that is first found to promote enlargement of individual mitochondria. PMID:26926452

  12. Variability of sporadic E-layer semi transparency (foEs-fbEs)with magnitude and distance from earthquake epicenters to vertical sounding stations

    NASA Astrophysics Data System (ADS)

    Liperovskaya, E. V.; Pokhotelov, O. A.; Hobara, Y.; Parrot, M.

    Variations of the Es-layer semi transparency co-efficient were analyzed for more than 100 earthquakes with magnitudes M > 4 and depths h < 100 km. Data of mid latitude vertical sounding stations (Kokubunji, Akita, and Yam-agawa) have been used for several decades before and after earthquake occurrences. The semi-transparency coefficient of Es-layer X = (foEs - fbEs)/fbEs can characterize, for thin layers, the presence of small scale plasma turbulence. It is shown that the turbulence level decreases by ~ 10% during three days before earthquakes probably due to the heating of the atmosphere. On the contrary, the turbulence level increases by the same value from one to three days after the shocks. For earthquakes with magnitudes M > 5 the effect exists at distances up to 300 km from the epicenters. The effect could also exist for weak (M ~ 4) and shallow (depth < 50 km) earthquakes at a distance smaller than 200 km from the epicenters.

  13. A Hard X-ray View on Two Distant VHE Blazars: 1ES 1101-232 and 1ES 1553+113

    SciTech Connect

    Reimer, A.; Costamente, L.; Madejski, G.; Reimer, O.; Dorner, D.; /Wurzburg U.

    2008-05-02

    TeV-blazars are known as prominent non-thermal emitters across the entire electromagnetic spectrum with their photon power peaking in the X-ray and TeV-band. If distant, absorption of -ray photons by the extragalactic background light (EBL) alters the intrinsic TeV spectral shape, thereby affecting the overall interpretation. Suzaku observations for two of the more distant TeV-blazars known to date, 1ES 1101-232 and 1ES 1553+113, were carried out in May and July 2006, respectively, including a quasi-simultaneous coverage with the state of the art Cherenkov telescope facilities. We report on the resulting data sets with emphasis on the X-ray band, and set into context to their historical behavior. During our campaign, we did not detect any significant X-ray or {gamma}-ray variability. 1ES 1101-232 was found in a quiescent state with the lowest X-ray flux ever measured. The combined XIS and HXD PIN data for 1ES 1101-232 and 1ES 1553+113 clearly indicate spectral curvature up to the highest hard X-ray data point ({approx} 30 keV), manifesting as softening with increasing energy. We describe this spectral shape by either a broken power law or a log-parabolic fit with equal statistical goodness of fits. The combined 1ES 1553+113 very high energy spectrum (90-500 GeV) did not show any significant changes with respect to earlier observations. The resulting contemporaneous broadband spectral energy distributions of both TeV-blazars are discussed in view of implications for intrinsic blazar parameter values, taking into account the {gamma}-ray absorption in the EBL.

  14. Enhanced thermotolerance and temperature-induced changes in protein composition in the hyperthermophilic archaeon ES4.

    PubMed Central

    Holden, J F; Baross, J A

    1993-01-01

    The hyperthermophilic archaeon ES4, a heterotrophic sulfur reducer isolated from a deep-sea hydrothermal vent, is capable of protecting itself from thermal stress at temperatures above its optimum for growth. The thermotolerance of ES4 was determined by exposing log-phase cells to various lethal high temperatures. When ES4 was shifted from 95 to 102 degrees C, it displayed recovery from an exponential rate of death, followed by transient thermotolerance. When ES4 was shifted directly from 95 to either 105 or 108 degrees C, only exponential death occurred. However, a shift from 95 to 105 degrees C with an intermediate incubation at 102 degrees C also gave ES4 transient thermotolerance to 105 degrees C. The protein composition of ES4 was examined at temperatures ranging from 75 to 102 degrees C by one-dimensional electrophoresis. Two proteins with molecular masses of approximately 90 and 150 kDa significantly decreased in abundance with increasing growth temperature, while a 98-kDa protein, present at very low levels at normal growth temperatures (76 to 99 degrees C), was more abundant at higher temperatures. The enhanced tolerance to hyperthermal conditions after a mild hyperthermal exposure and the increased abundance of the 98-kDa protein at above-optimal temperatures imply that ES4 is capable of a heat shock-like response previously unseen in hyperthermophilic archaea. Images PMID:8491704

  15. The novel elicitor AsES triggers a defense response against Botrytis cinerea in Arabidopsis thaliana.

    PubMed

    Hael-Conrad, V; Abou-Mansour, E; Díaz-Ricci, J-C; Métraux, J-P; Serrano, M

    2015-12-01

    AsES (Acremonium strictum Elicitor and Subtilisin) is a novel extracellular elicitor protein produced by the avirulent isolate SS71 of the opportunist strawberry fungal pathogen A. strictum. Here we describe the activity of AsES in the plant-pathogen system Arabidopsis thaliana-Botrytis cinerea. We show that AsES renders A. thaliana plants resistant to the necrotrophic pathogen B. cinerea, both locally and systemically and the defense response observed is dose-dependent. Systemic, but not local resistance is dependent on the length of exposure to AsES. The germination of the spores in vitro was not inhibited by AsES, implying that protection to B. cinerea is due to the induction of the plant defenses. These results were further supported by the findings that AsES differentially affects mutants impaired in the response to salicylic acid, jasmonic acid and ethylene, suggesting that AsES triggers the defense response through these three signaling pathways.

  16. The German Environmental Survey 1990/1992 (GerES II): a representative population study.

    PubMed

    Seifert, B; Becker, K; Hoffmann, K; Krause, C; Schulz, C

    2000-01-01

    The German Environmental Survey (GerES) is a large-scale population study which has repeatedly been carried out in Germany. GerES I was conducted in 1985/1986 followed by GerES IIa in 1990/1991 (West Germany) and GerES IIb in 1991/1992 (East Germany). GerES III is currently run in both parts of Germany. The main goal of the surveys is to analyse and document the extent, distribution and determinants of the exposure to environmental pollutants of the German general population. Field work is conducted using a combination of several tools, including questionnaires, interviews, human biomonitoring, and indoor and outdoor environmental samplings. This paper describes the design of GerES II, and gives a general outline of the field work and the analytical procedures used. In GerES II, about 4000 adults were representatively selected from the German population with regard to age, gender and community size. Approximately 700 children were also included. Arsenic, cadmium, copper, chromium, lead, and mercury were determined in blood, morning urine and/or scalp hair. In addition, samples were taken and analysed to characterise exposure in the domestic environment (indoor air, house dust, drinking water). The contribution to exposure of food was also studied. Exposure to volatile organic compounds (VOCs) was determined by personal sampling.

  17. The Carbohydrate-linked Phosphorylcholine of the Parasitic Nematode Product ES-62 Modulates Complement Activation*

    PubMed Central

    Ahmed, Umul Kulthum; Maller, N. Claire; Iqbal, Asif J.; Al-Riyami, Lamyaa; Harnett, William; Raynes, John G.

    2016-01-01

    Parasitic nematodes manufacture various carbohydrate-linked phosphorylcholine (PCh)-containing molecules, including ES-62, a protein with an N-linked glycan terminally substituted with PCh. The PCh component is biologically important because it is required for immunomodulatory effects. We showed that most ES-62 was bound to a single protein, C-reactive protein (CRP), in normal human serum, displaying a calcium-dependent, high-avidity interaction and ability to form large complexes. Unexpectedly, CRP binding to ES-62 failed to efficiently activate complement as far as the C3 convertase stage in comparison with PCh-BSA and PCh-containing Streptococcus pneumoniae cell wall polysaccharide. C1q capture assays demonstrated an ES-62-CRP-C1q interaction in serum. The three ligands all activated C1 and generated C4b to similar extents. However, a C2a active site was not generated following ES-62 binding to CRP, demonstrating that C2 cleavage was far less efficient for ES-62-containing complexes. We proposed that failure of C2 cleavage was due to the flexible nature of carbohydrate-bound PCh and that reduced proximity of the C1 complex was the reason that C2 was poorly cleaved. This was confirmed using synthetic analogues that were similar to ES-62 only in respect of having a flexible PCh. Furthermore, ES-62 was shown to deplete early complement components, such as the rate-limiting C4, following CRP interaction and thereby inhibit classical pathway activation. Thus, flexible PCh-glycan represents a novel mechanism for subversion of complement activation. These data illustrate the importance of the rate-limiting C4/C2 stage of complement activation and reveal a new addition to the repertoire of ES-62 immunomodulatory mechanisms with possible therapeutic applications. PMID:27044740

  18. Hyperfine field of einsteinium in iron and nuclear magnetic moment of {sup 254}Es

    SciTech Connect

    Severijns, N.; Kraev, I. S.; Phalet, T.; Tandecki, M.; Traykov, E.; Gorp, S. Van; Wauters, F.; Belyaev, A. A.; Lukhanin, A. A.; Noga, V. I.; Erzinkyan, A. L.; Parfenova, V. P.; Eversheim, P.-D.; Herzog, P.; Tramm, C.; Filimonov, V. T.; Toporov, Yu. G.; Zotov, E.; Golovko, V. V.; Gurevich, G. M.

    2009-06-15

    The angular distributions of {gamma} rays and {alpha} particles from oriented {sup 250}Bk, {sup 253,254}Es, and {sup 255}Fm nuclei were investigated to extract hyperfine interaction information for these actinide impurities in an iron host lattice. The hyperfine field of einsteinium in iron was found to be |B{sub hf}(EsFe{sub lowbar|})=396(32) T. With this value the magnetic moment of {sup 254}Es was then determined as |{mu}|=4.35(41) {mu}{sub N}.

  19. Circuit for connecting the Videoton-340 with the ES-1030 as an operator console

    NASA Technical Reports Server (NTRS)

    Uskov, V. A.; PRYADIN

    1979-01-01

    A system of connection of the Videoton-340 text display to the standard ES-7070 unit, for use as an operator console, partially replacing and supplementing the ES-7070 electric typewriter, is described. The interactions, including the specific instructions, among the Videoton-340, the Consul-260.1 electric typewriter and the ES-7070, which is the means of user access to the unified system of computers, are presented. Users at the Institute of Space Research note the reliability, high information output rate, noiselessness and convenience of the keyboard of the system.

  20. A murine-ES like state facilitates transgenesis and homologous recombination in human pluripotent stem cells

    PubMed Central

    Buecker, Christa; Chen, Hsu-Hsin; Polo, Jose; Daheron, Laurence; Bu, Lei; Barakat, Tahsin Stefan; Okwieka, Patricia; Porter, Andrew; Gribnau, Joost; Hochedlinger, Konrad; Geijsen, Niels

    2010-01-01

    Murine embryonic stem cells have been shown to exist in two functionally distinct pluripotent states, embryonic stem cells (ES cell)- and epiblast stem cells (EpiSCs), which are defined by the culture growth factor conditions. Human ES cells appear to exist in an epiblast-like state, which in comparison to their murine counterparts, is relatively difficult to propagate and manipulate. As a result, gene targeting is difficult and to-date only a handful of human knock-in or knock-out cell lines exist. We explored whether an alternative stem cell state exists for human stem cells as well, and demonstrate that manipulation of the growth factor milieu allows the derivation of a novel human stem cell type that displays morphological, molecular and functional properties of murine ES cells and facilitates gene targeting. As such, the murine ES-like state provides a powerful tool for the generation of recombinant human pluripotent stem cell lines. PMID:20569691

  1. Destructive Testing of an ES-3100 Shipping Container at the Savannah River National Laboratory

    SciTech Connect

    Loftin, B.; Abramczyk, G.

    2015-06-09

    Destructive testing of an ES-3100 Shipping Container was completed by the Packaging Technology and Pressurized Systems organization within the Savannah River National Laboratory in order to qualify the ES-3100 as a candidate storage and transport package for applications at various facilities at the Savannah River Site. The testing consisted of the detonation of three explosive charges at separate locations on a single ES-3100. The locations for the placement were chosen based the design of the ES-3100 as well as the most likely places for the package to incur damage as a result of the detonation. The testing was completed at an offsite location, which raised challenges as well as allowed for development of new partnerships for this testing and for potential future testing. The results of the testing, the methods used to complete the testing, and similar, potential future work will be discussed.

  2. BootES: an R package for bootstrap confidence intervals on effect sizes.

    PubMed

    Kirby, Kris N; Gerlanc, Daniel

    2013-12-01

    Bootstrap Effect Sizes (bootES; Gerlanc & Kirby, 2012) is a free, open-source software package for R (R Development Core Team, 2012), which is a language and environment for statistical computing. BootES computes both unstandardized and standardized effect sizes (such as Cohen's d, Hedges's g, and Pearson's r) and makes easily available for the first time the computation of their bootstrap confidence intervals (CIs). In this article, we illustrate how to use bootES to find effect sizes for contrasts in between-subjects, within-subjects, and mixed factorial designs and to find bootstrap CIs for correlations and differences between correlations. An appendix gives a brief introduction to R that will allow readers to use bootES without having prior knowledge of R.

  3. Discovery of the long hunted blazar 1ES 0033+595 by the MAGIC telescopes

    NASA Astrophysics Data System (ADS)

    Uellenbeck, M.; Lombardi, S.; Mankuzhiyil, N.; Palatiello, M.; Persic, M.; Tronconi, V.; Buson, S.; MAGIC Collaboration; Fermi-LAT Collaboration

    2012-12-01

    We report the discovery of Very High Energy (VHE, E>100 GeV) emission from 1ES 0033+595 with the MAGIC telescopes. 1ES 0033+595 is a high-frequency peaked BL Lac object with still unknown redshift. Together with the simultaneous Fermi LAT results we present for the first time the reconstructed inverse-compton peak of the SED of this new VHE γ-ray emitter.

  4. Intravenous Administration of Human ES-derived Neural Precursor Cells Attenuates Cuprizone-induced CNS Demyelination

    PubMed Central

    Crocker, Stephen J.; Bajpai, Ruchi; Moore, Craig S.; Frausto, Ricardo F.; Brown, Graham D.; Pagarigan, Roberto R.; Whitton, J. Lindsay; Terskikh, Alexey V.

    2011-01-01

    Aims Previous studies have demonstrated the therapeutic potential for human embryonic stem cell-derived neural precursor cells (hES-NPCs) in autoimmune and genetic animal models of demyelinating diseases. Herein, we tested whether intravenous (i.v) administration of hES-NPCs would impact central nervous system (CNS) demyelination in a cuprizone model of demyelination. Methods C57Bl/6 mice were fed cuprizone (0.2%) for two weeks and then separated into two groups that either received an i.v. injection of hES-NPCs or i.v. administration of media without these cells. After an additional two weeks of dietary cuprizone treatment, CNS tissues were analyzed for detection of transplanted cells and differences in myelination in the region of the corpus callosum (CC). Results Cuprizone-induced demyelination in the CC was significantly reduced in mice treated with hES-NPCs compared with cuprizone-treated controls that did not receive stem cells. hES-NPCs were identified within the brain tissues of treated mice and revealed migration of transplanted cells into the CNS. A limited number of human cells were found to express the mature oligodendrocyte marker, O1, or the astrocyte marker, GFAP. Reduced apoptosis and attenuated microglial and astrocytic responses were also observed in the CC of hES-NPC-treated mice. Conclusions These findings indicated that systemically-administered hES-NPCs migrated from circulation into a demyelinated lesion within the CNS and effectively reduced demyelination. Observed reductions in astrocyte and microglial responses, and (c) the benefit of hES-NPC treatment in this model of myelin injury was not obviously accountable to tissue replacement by exogenously administered cells. PMID:21276029

  5. Regulation of ES cell differentiation by functional and conformational modulation of p53.

    PubMed Central

    Sabapathy, K; Klemm, M; Jaenisch, R; Wagner, E F

    1997-01-01

    Embryonic stem (ES) cell lines were used to examine the role of p53 during in vitro differentiation. Undifferentiated ES cells express high levels of p53 exclusively in the wild-type conformation, immunoprecipitable by monoclonal antibody PAb246, and p53 was found to be functionally active as determined by its ability to bind DNA specifically and to activate transcription of target genes. Differentiation in vitro resulted in a decrease in the levels of p53 and in a shift in its conformational status to the mutant form, detectable by monoclonal antibody PAb240, with a concomitant loss of functional activity. The presence of functional p53 in the undifferentiated ES cells renders them hypersensitive to UV irradiation, whereas the differentiated cells were resistant to UV treatment. ES cells lacking p53 exhibit enhanced proliferation in both the undifferentiated and differentiated state, and apoptosis accompanying differentiation was found to be reduced. Furthermore, wild-type ES cells undergoing apoptosis expressed functional p53. Expression of the temperature-sensitive p53val135 mutant in wild-type ES cells resulted in a reduction of apoptosis accompanying differentiation when it adopted a mutant conformation at 39 degrees C. These data demonstrate that functional inactivation of p53 allows differentiating cells to escape from apoptosis, and suggest that the conformational switch could regulate the inactivation process. PMID:9321401

  6. Chaperonin GroEL-GroES Functions as both Alternating and Non-Alternating Engines.

    PubMed

    Yamamoto, Daisuke; Ando, Toshio

    2016-07-31

    A double ring-shaped GroEL consisting of 14 ATPase subunits assists protein folding, together with co-chaperonin GroES. The dynamic GroEL-GroES interaction is actively involved in the chaperonin reaction. Therefore, revealing this dynamic interaction is a key to understanding the operation principle of GroEL. Nevertheless, how this interaction proceeds in the reaction cycle has long been controversial. Here, we directly imaged GroEL-GroES interaction in the presence of disulfide-reduced α-lactalbumin as a substrate protein using high-speed atomic force microscopy. This real-time imaging revealed the occurrence of primary, symmetric GroEL:GroES2 and secondary, asymmetric GroEL:GroES1 complexes. Remarkably, the reaction was observed to often branch into main and side pathways. In the main pathway, alternate binding and release of GroES occurs at the two rings, indicating tight cooperation between the two rings. In the side pathway, however, this cooperation is disrupted, resulting in the interruption of alternating rhythm. From various properties observed for both pathways, we provide mechanistic insight into the alternate and non-alternate operations of the two-engine system.

  7. Suppression of pervasive noncoding transcription in embryonic stem cells by esBAF

    PubMed Central

    Hainer, Sarah J.; Gu, Weifeng; Carone, Benjamin R.; Landry, Benjamin D.; Rando, Oliver J.; Mello, Craig C.

    2015-01-01

    Approximately 75% of the human genome is transcribed, the majority of which does not encode protein. However, many noncoding RNAs (ncRNAs) are rapidly degraded after transcription, and relatively few have established functions, questioning the significance of this observation. Here we show that esBAF, a SWI/SNF family nucleosome remodeling factor, suppresses transcription of ncRNAs from ∼57,000 nucleosome-depleted regions (NDRs) throughout the genome of mouse embryonic stem cells (ESCs). We show that esBAF functions to both keep NDRs nucleosome-free and promote elevated nucleosome occupancy adjacent to NDRs. Reduction of adjacent nucleosome occupancy upon esBAF depletion is strongly correlated with ncRNA expression, suggesting that flanking nucleosomes form a barrier to pervasive transcription. Upon forcing nucleosome occupancy near two NDRs using a nucleosome-positioning sequence, we found that esBAF is no longer required to silence transcription. Therefore, esBAF’s function to enforce nucleosome occupancy adjacent to NDRs, and not its function to maintain NDRs in a nucleosome-free state, is necessary for silencing transcription over ncDNA. Finally, we show that the ability of a strongly positioned nucleosome to repress ncRNA depends on its translational positioning. These data reveal a novel role for esBAF in suppressing pervasive transcription from open chromatin regions in ESCs. PMID:25691467

  8. Studies of electronic configurations in the emission spectra of lanthanides and actinides: application to the interpretation of Es I and Es II, predictions for Fm I

    SciTech Connect

    Wyart, Jean-Francois . E-mail: jean-francois.wyart@lac.u-psud.fr; Blaise, Jean; Worden, Earl F.

    2005-02-15

    The interpretation of the spectra of free atoms and gaseous ions in the 4f{sup N} and 5f{sup N} periods became less active after critical compilations of energy levels appeared. However, several spectra are still under study and the application of the Racah-Slater and HFR methods to extended sets of configurations leads to revisions and additions. In doubly charged ions of lanthanides, the treatment of configuration interaction by means of effective parameters and by extension of the basis of states are both important. Concerning actinides, calculations of several observables (Lande factors and isotope shifts in Pu I, hyperfine constants, transition probabilities) prove the quality of eigenfunctions. The classification of Es I and Es II has been extended and radial parameters for fine and hyperfine structures have been derived. Level predictions for the next element fermium are supported by parameter extrapolations.

  9. The University of Arizona Astronomy Club Observations of Transiting Extrasolar Planets TrES-3b and TrES-4b

    NASA Astrophysics Data System (ADS)

    Turner, Jake; Hardegree-Ullman, K.; Smart, B.; Walker-LaFollette, A.; Cunningham, K.; Hardegree-Ullman, E. E.; Crawford, B.; Mueting, J.; Carleton, T.; Schwarz, K.; Robertson, A.; Guvenen, B.; Towner, A.; Austin, C.; Henz, T.; Keys, D.; Johnson, K.

    2011-05-01

    Using the Steward Observatory 61" Kuiper Telescope, The University of Arizona Astronomy Club observed extrasolar planets TrES-3b and TrES-4b. We observed the planets with the Harris-B, V, and R filters as they transited their parent stars during the months of May-July 2009. The main goal of this project was to get undergraduates involved with a research astronomy project and allow them to gain experience beyond what they would receive in the classroom. Many of the team members were introduced to astronomical observing techniques and data reduction using IRAF. Part of the project involved determining the optimum number of flat-field and bias frames required for image calibrations. With our results, we have been able to confirm and refine previously published values for the planets' orbital inclination, mass, radius, and density.

  10. Identification of Bacteria in Pasteurized Zucchini Purées Stored at Different Temperatures and Comparison with Those Found in Other Pasteurized Vegetable Purées

    PubMed Central

    Guinebretiere, Marie-Hélène; Berge, Odile; Normand, Philippe; Morris, Cindy; Carlin, Frédéric; Nguyen-The, Christophe

    2001-01-01

    One hundred nineteen isolates from a commercial zucchini purée stored at 4, 10, and 20 to 25°C were fingerprinted using repetitive sequence-based PCR (REP-PCR) and classified into 35 REP types. One representative isolate of each REP type was subsequently identified by API50CHB/20E profile and partial rrs gene sequence analysis. Nine REP types were misidentified by the API system. Strains were misidentified as being in the Bacillus circulans (group 2) API taxon or in taxa with a low number of positive API characters such as Brevibacillus brevis. A phylogenetic analysis pointed to one new species of Bacillus and three new species of Paenibacillus among the misidentified REP types. Bacterial components in zucchini purée were compared phenotypically with those obtained in previous work on broccoli, carrot, leek, potato, and split pea purées, based on simple matching coefficient and unweighted pair group method with averages cluster analysis. Out of 254 strains, 69 strains previously identified as B. circulans (group 2) or B. circulans/B. macerans/B. polymyxa were assigned to a new Paenibacillus taxon phylogenetically related to P. azotofixans. Storage conditions at 4°C favored the development of “B. macroides/B. maroccanus” and Paenibacillus spp. in zucchini purées and Paenibacillus spp. in other purées. Storage conditions at 20 to 25°C favored the development of B. subtilis group (B. licheniformis and B. subtilis) and B. cereus group strains. At 10°C, Paenibacillus spp. were always present at high frequencies, whereas the occurrence of B. macroides/B. maroccanus (in zucchini purées), B. cereus, and B. pumilus varied with the experiment. PMID:11571151

  11. Targeted Vezf1 null mutation impairs vascular structure formation during ES cell differentiation

    PubMed Central

    Zou, Zhongmin; Ocaya, Pauline A.; Sun, Huiquin; Kuhnert, Frank; Stuhlmann, Heidi

    2010-01-01

    Objective Vezf1 is an early zinc finger transcription factor that is essential for normal vascular development and functions in a dose-dependent manner. Here, we investigated the role of Vezf1 during processes of endothelial cell differentiation and maturation by studying mutant Vezf1 ES cells using the in vitro embryoid body differentiation model and the in vivo teratocarcinoma model. Methods and Results Vezf1−/− ES cell-derived embryoid bodies failed to form a well-organized vascular network and showed dramatic vascular sprouting defects. Our results indicate that the retinol pathway is an important mediator of Vezf1 function, and that loss of Vezf1 results in reduced retinol/Vitamin A signaling and aberrant extracellular matrix formation. Unexpectedly, we also uncovered defects during in vitro differentiation of Vezf1−/− ES cells along hematopoietic cell lineages. Vezf1−/− ES cell-derived teratocarcinomas were able to spontaneously differentiate into cell types of all three germ layers. However, histological and immunohistochemical examination of these tumors showed decreased cell proliferation, delayed differentiation, and large foci of cells with extensive deposition of extracellular matrix. Embryoid bodies and teratocarcinomas derived from heterozygous ES cells displayed an intermediate phenotype. Conclusion Together, these results suggest that Vezf1 is involved in early differentiation processes of the vasculature by regulating cell differentiation, proliferation, and ECM distribution and deposition. PMID:20431070

  12. Coupling between parameters of Es layer and planetary waves during SSW 2008, 2010

    NASA Astrophysics Data System (ADS)

    Korenkova, N. A.; Leschenko, V. S.; Cherniak, Iu. V.; Korenkov, Yu. N.

    2015-11-01

    We report the features of the critical frequency foEs and probability of the sporadic E layer occurrence P(Es) estimated at the Kaliningrad ionosonde station (54°N, 20°E) during the sudden stratosphere warming (SSW) in winter during the years 2008 and 2010. Additionally we use the neutral atmosphere (temperature, wind circulation data at 10 hPa) data for the same temporal interval. Considered periods were characterized by the low solar and geomagnetic activities. We investigate the indirect relation of the neutral temperature in the stratosphere with the long-term variability in ionospheric Es layer parameters during SSW. We found a rather good correlation between the Es layer parameters and the stratospheric temperature increase during the SSW events. We used the continuous wavelet transforms in order to demonstrate the relationship between planetary wave (PW) activity and temporal variability of the sporadic E-layer parameters during SSW 2008 and SSW 2010. We detect a significant wave-like oscillation with periods covering a planetary oscillation in both Es layer parameters. Generally, the oscillations were observed in two bands that corresponded to the periods of 8-10 days before SSW and 12-16 and more days' band during SSW.

  13. Effects of nuclear transfer procedures on ES cell cloning efficiency in the mouse.

    PubMed

    Yabuuchi, Akiko; Yasuda, Yoshiko; Kato, Yoko; Tsunoda, Yukio

    2004-04-01

    Enucleated oocytes receiving mouse embryonic stem (ES) cells develop into fertile young. The developmental potential to young is low, however, and the rate of postnatal death is high. We examined the effect of various nuclear transfer procedures on the in vitro and in vivo developmental potential of nuclear-transferred oocytes. The potential of oocytes receiving ES cells at M phase to develop into blastocysts after fusion by Sendai virus was high compared with that after direct injection (67% vs. 30%). The developmental potential of oocytes receiving ES cells at the M phase is higher than that of oocytes receiving ES cells at the G(1) phase (30-67% vs. 2-5%). Developmental ability to live young was low in all groups (0-4%). Different activation protocols affected the potential to develop into blastocysts to a different extent (27-62%), but did not affect the potential to develop into live young (0-3%). The present study demonstrated that the various conditions examined did not affect the potential of nuclear-transferred oocytes receiving ES cells to develop into live young or the incidence of postnatal death.

  14. Dct::lacZ ES cells: a novel cellular model to study melanocyte determination and differentiation.

    PubMed

    Pla, Patrick; Solov'eva, Olga; Moore, Robert; Alberti, Christophe; Kunisada, Takahiro; Larue, Lionel

    2004-04-01

    Embryonic stem (ES) cells differentiate into various cell lineages in vitro. A procedure was previously designed to promote the differentiation of ES cells towards the melanocyte lineage and to obtain large and reproducible amounts of melanocytes. To elucidate the main events that lead to the development of melanocytes in vitro, we used transgenic Dct::lacZ mouse blastocysts to establish ES cell lines expressing the lacZ reporter gene under the control of the Dct promoter. Dct, a melanoblast marker, is expressed just after melanoblast determination in vivo. We evaluated the importance of recruitment, proliferation and differentiation during melanocyte ontogeny after the in vitro differentiation of Dct::lacZ ES cells into melanocytes. We showed that bFGF and cholera toxin induce precocious melanoblast determination, associated with early melanocyte differentiation. Edn3 induced melanoblast proliferation and long-term melanoblast recruitment, but not precocious determination. The lack of basic Fibroblast Growth Factor (bFGF) and cholera toxin can be partially compensated by Edn3. Thus, Dct::lacZ ES cells can be used as a model to study determination, proliferation and differentiation in the melanocyte lineage in vitro.

  15. Bacteriophage-encoded cochaperonins can substitute for Escherichia coli’s essential GroES protein

    PubMed Central

    Keppel, France; Rychner, Monique; Georgopoulos, Costa

    2002-01-01

    The Escherichia coli chaperonin machine is composed of two members, GroEL and GroES. The GroEL chaperonin can bind 10–15% of E. coli’s unfolded proteins in one of its central cavities and help them fold in cooperation with the GroES cochaperonin. Both proteins are absolutely essential for bacterial growth. Several large, lytic bacteriophages, such as T4 and RB49, use the host-encoded GroEL in conjunction with their own bacteriophage-encoded cochaperonin for the correct assembly of their major capsid protein, suggesting a cochaperonin specificity for the in vivo folding of certain substrates. Here, we demonstrate that, when the cochaperonin of either bacteriophage T4 (Gp31) or RB49 (CocO) is expressed in E. coli, the otherwise essential groES gene can be deleted. Thus, it appears that, despite very little sequence identity with groES, the bacteriophage-encoded Gp31 and CocO proteins are capable of replacing GroES in the folding of E. coli’s essential, housekeeping proteins. PMID:12189177

  16. Environment, Safety, and Health (ES&H) self-assessment guide

    SciTech Connect

    Reese, R.T.; Golden, N.L.; Romero, J.R.; Yesner, S.

    1997-06-01

    This document has been prepared as a guide for conducting self-assessments of ES&H functional programs and organizational (line) implementation of these programs. This guide is intended for use by individuals and/or teams involved in or familiar with ES&H programs and line operations (e.g., the {open_quotes}self{close_quotes}in self-assessment). Essential elements of the self-assessment process are described including: schedule and priorities, scope and approach, assessment criteria (e.g., performance objectives and measures), information gathering and analysis techniques, and documentation of planning efforts and results. The appendices in this guide include: (1) an assessment prioritization process, (2) generic performance objectives for line implementation and for ES&H functional programs, (3) sources for ES&H assessment information, (4) systemic factors (developed for SNL`s root cause analysis program), (5) Lockheed Martin audit questions for management systems, compliance and validation, and specific areas and concerns, (6) DOE facility representatives checklist, and (7) assessment tools and resources developed at SNL and other DOE/Lockheed Martin sites. This document is a product of the efforts associated with the SNL ES&H Oversight Pilot Project conducted from June 1995 to January 1997. This Pilot was part of the overall initiative by DOE to reduce burdensome agency oversight by placing greater reliance on contractor self-assessment.

  17. Solar Eclipse Effects of 22 July 2009 on Es-layer

    NASA Astrophysics Data System (ADS)

    Chen, G.; Zhao, Z.; Yang, G.

    2013-12-01

    The Sporadic-E frequently emerging in mid-latitude during summer is a very special layer in the ionosphere and its formation mechanism is different to other layers. The total solar eclipse of 22 July 2009 provided a very unique opportunity to study the relationship of Es and solar radiation. During the solar eclipse day and the days before and after, the vertical incidence ionosonde was located in Wuhan to record the ionograms for this event. Two oblique incidence high-frequency radio systems were used to record the waves from Wuhan to Suzhou and from Wuhan to Huaian. The enhancement of Es during the eclipse period was observed in the vertical and oblique incidence ionograms. The quasi-periodic fluctuations in the critical frequency and Doppler frequency shift curves indicated the possible existence of the gravity waves, which may be responsible for the Es enhancement. However, we find that the enhancement occurred earlier than the appearance of gravity waves and consider that there may be other mechanisms which contribute to the observed enhancement in the ionosphere. A hypothesis is put forward that the cooling effect of the lunar shadow induced powerful airflow flowing from the northern and southern limits of the shadow towards its center, which accelerated the irregularities in Es to produce the large-scale Doppler shift in the reflected waves and form the meridional windshear. Both the windshear and the gravity waves may affect the Es layer and increase the electron concentration. Many observed phenomena are in accordance with this.

  18. Establishment of key grid-connected performance index system for integrated PV-ES system

    NASA Astrophysics Data System (ADS)

    Li, Q.; Yuan, X. D.; Qi, Q.; Liu, H. M.

    2016-08-01

    In order to further promote integrated optimization operation of distributed new energy/ energy storage/ active load, this paper studies the integrated photovoltaic-energy storage (PV-ES) system which is connected with the distribution network, and analyzes typical structure and configuration selection for integrated PV-ES generation system. By combining practical grid- connected characteristics requirements and technology standard specification of photovoltaic generation system, this paper takes full account of energy storage system, and then proposes several new grid-connected performance indexes such as paralleled current sharing characteristic, parallel response consistency, adjusting characteristic, virtual moment of inertia characteristic, on- grid/off-grid switch characteristic, and so on. A comprehensive and feasible grid-connected performance index system is then established to support grid-connected performance testing on integrated PV-ES system.

  19. Folding of malate dehydrogenase inside the GroEL-GroES cavity.

    PubMed

    Chen, J; Walter, S; Horwich, A L; Smith, D L

    2001-08-01

    The chaperonin GroEL binds nonnative substrate protein in the hydrophobic central cavity of an open ring. ATP and GroES binding to the same ring converts this cavity into an encapsulated, hydrophilic chamber that mediates productive folding. A 'rack' mechanism of initial protein unfolding proposes that, upon GroES and ATP binding, the polypeptide is stretched between the binding sites on the twisting apical domains of GroEL before complete release into the chamber. Here, the structure of malate dehydrogenase (MDH) subunit during folding is monitored by deuterium exchange, peptic fragment production and mass spectrometry. When bound to GroEL, MDH exhibits a core of partially protected secondary structure that is only modestly deprotected upon ATP and GroES binding. Moreover, deprotection is broadly distributed throughout MDH, suggesting that it results from breaking hydrogen bonds between MDH and the cavity wall or global destabilization, as opposed to forced mechanical unfolding.

  20. Proposed production of a large (approx. 40. mu. g) sample of /sup 254/Es

    SciTech Connect

    Bigelow, J.E.; Alexander, C.W.; King, L.J.

    1985-01-01

    A recent workshop sponsored by the National Research Council has made it clear that the key to further substantial progress in heavy element research is the expanded use of 276-day /sup 254/Es as target material. Einsteinium-254 has the greatest mass and charge of any nuclide that can be produced in the required multimicrogram quantities in the foreseeable future. Four major laboratories (Lawrence Berkeley Laboratory, Lawrence Livermore National Laboratory, Los Alamos National Laboratory, and Oak Ridge National Laboratory (ORNL)) active in transplutonium research have collaborated to propose a major new thrust in transplutonium research that will require an order of magnitude more /sup 254/Es than is normally available. This project, called LEAP (an acronym for Large Einsteinium Activation Program) has goals of determining the inorganic chemistry and nuclear chemistry and physics of the transeinsteinium elements through atomic number 109, plus a search for superheavy elements. LEAP is based on using approx.40-..mu..g of /sup 254/Es as a target for heavy-ion accelerators. The Tranuranium Processing Plant (TRU) of the Chemical Technology Division of ORNL has been given the task of determining the feasibility of producing a 40 ..mu..g sample of /sup 254/Es and, if later requested, of actually producing the sample. This task, which has been under way for several years, is directed toward three areas of investigation: (1) experimental determination of the neutron cross sections of certain transplutonium isotopes important to the production of /sup 254/Es; (2) selection of a /sup 254/Es production scheme; and (3) development of the necessary hardware, followed by an actual test irradiation. 12 refs., 9 figs., 2 tabs.

  1. The Lawrence Berkeley National Laboratory ES&H self-assessmentprogram

    SciTech Connect

    Chernowski, John G.

    2003-02-11

    Lawrence Berkeley National Laboratory (Berkeley Lab) is a multiprogram national research facility operated by the University of California for the U.S. Department of Energy (DOE). DOE environment, safety, and health (ES&H) policy requires that all Berkeley Lab work be performed safely, with minimal adverse impact on the public and the environment. To facilitate safe and responsible work, Berkeley Lab divisions, directorates, and select departments must develop and implement Integrated Safety Management (ISM) plans. Berkeley Lab operates a formal internal ES&H self-assessment process to evaluate ES&H programs and policies and assure that ISM is implemented at all levels of activities and operations. ISM requires that: (1) work is defined, (2) hazards are identified, (3) controls are developed and implemented, (4) work is performed as authorized, and (5) feedback and improvement are continuous. These five ISM core functions are sustained by applying the seven guiding principles of ISM. These are: (1) line management responsibility and accountability for ES&H, (2) clear ES&H roles and responsibilities, (3) competency commensurate with responsibilities, (4) an ongoing balance between safety on the one hand and research and operational priorities on the other, (5) identification of standards and requirements, (6) hazard controls tailored to the work, and (7) operations authorization. Self-assessment at Berkeley Lab is a continuous process of information gathering and evaluation. The goals of the self-assessment program are: (a) Ensure that work activities and operations are done safely and in a manner that maximizes public and environmental protection. (b) Ensure that the five core functions and seven guiding principles of integrated safety management are employed effectively in work planning and performance. (c) Meet regulatory requirements for DOE oversight, self-assessment, and an integrated safety management system. (d) Meet contractual requirements for ES

  2. Identification and quantification of components in extracts of Uncaria tomentosa by HPLC-ES/MS.

    PubMed

    Montoro, P; Carbone, V; Quiroz, J de Dioz Zuniga; De Simone, F; Pizza, C

    2004-01-01

    The two main classes of secondary metabolites, alkaloids and quinovic acid glycosides, of Uncaria tomentosa (Willd.) DC. (Rubiaceae), a Peruvian plant commonly known as 'uña de gato', have been analysed. Separation of the alkaloidal fraction was achieved using a solid phase extraction method based on cationic exchange, and an analytical method employing HPLC-ES/MS has been developed. Quantitative data for commercial wild bark, cultivated bark and leaves are reported. The analysis of quinovic acid glycosides was performed directly on the crude extract using both a fast analytical method based on flow injection ES/MS, and a more complete analytical technique using HPLC-MS.

  3. El fenotipo de las mucinas en el esófago de Barrett

    PubMed Central

    Torrado, Julio; Piazuelo, María Blanca; Ruiz, Irune; Izarzugaza, María Isabel; Camargo, María Constanza; Delgado, Alberto; Abdirad, Afshin; Correa, Pelayo

    2011-01-01

    Antecedentes El esófago de Barrett es una reconocida lesión precursora de adenocarcinoma esofágico. Aunque generalmente asociada al reflujo gastroesofágico, los mecanismos patogénicos de la enfermedad no son bien conocidos. El objetivo del presente estudio es explorar la historia natural e identificar marcadores de progreso del proceso precanceroso. Material y métodos Se utilizaron cortes histológicos de 67 especímenes de esófago correspondientes a 14 pacientes con esófago de Barrett, a los que se siguió entre 1 – 9 años. Se clasificaron las lesiones en: esófago de Barrett sin displasia, indefinido para displasia o con displasia. Se evaluó la expresión de diferentes mucinas en las células caliciformes y en las columnares usando técnicas de histoquímica e inmunohistoquímica. Resultados En todos los casos se comprobó la presencia de metaplasia intestinal incompleta. Las células columnares dentro del epitelio metaplásico contenían mucinas neutras. A mayor severidad de la lesión se encontró significativamente menor expresión de sialomucinas en las células columnares (p de tendencia igual a 0,03). En sujetos con lesiones indefinidas para displasia se observó un mayor contenido de sulfomucinas en las células caliciformes (p=0,034) y de MUC2 en las células columnares (p=0,029) que en sujetos con esófago de Barrett sin displasia. Se observó expresión de la mucina intestinal MUC2 y de la mucina gástrica MUC5AC en todas las muestras. MUC6, una mucina de las glándulas profundas gástricas, se presentó ocasionalmente. Conclusión La evaluación de los perfiles de mucinas en el esófago de Barrett sugiere una transición gradual del fenotipo del epitelio metaplásico a medida que la lesión avanza en el tiempo. PMID:21804831

  4. Identification and quantification of components in extracts of Uncaria tomentosa by HPLC-ES/MS.

    PubMed

    Montoro, P; Carbone, V; Quiroz, J de Dioz Zuniga; De Simone, F; Pizza, C

    2004-01-01

    The two main classes of secondary metabolites, alkaloids and quinovic acid glycosides, of Uncaria tomentosa (Willd.) DC. (Rubiaceae), a Peruvian plant commonly known as 'uña de gato', have been analysed. Separation of the alkaloidal fraction was achieved using a solid phase extraction method based on cationic exchange, and an analytical method employing HPLC-ES/MS has been developed. Quantitative data for commercial wild bark, cultivated bark and leaves are reported. The analysis of quinovic acid glycosides was performed directly on the crude extract using both a fast analytical method based on flow injection ES/MS, and a more complete analytical technique using HPLC-MS. PMID:14979528

  5. Transfer cross sections from reactions with /sup 254/Es as a target

    SciTech Connect

    Schadel, M.; Bruechle, W.; Bruegger, M.; Gaggeler, H.; Moody, K.J.; Schardt, D.; Suemmerer, K.; Hulet, E.K.; Dougan, A.D.; Dougan, R.J.

    1986-04-01

    We report radiochemically determined cross sections for the heaviest known actinides produced in transfer reactions of /sup 16,18/O and /sup 22/Ne with /sup 254/Es as a target. A comparison with data for similar transfers from /sup 248/Cm targets is made. Transfer cross sections are extrapolated for the production of the unknown, neutron-rich isotopes of elements 101 through 105, and the unique potential of /sup 254/Es as a target to make these exotic nuclei accessible is demonstrated.

  6. The Nature of the Enigmatic 10-Minute Accreting Binary System ES CET

    NASA Technical Reports Server (NTRS)

    Steeghs, Daniel

    2005-01-01

    ES Cet is one of the most compact binary systems known with an orbital period of only 10.3 minutes. Our allocated observations with the XMM-Newton X-ray satellite were performed in January and July 2004, with the data being delivered to the PI in August 2004. Preliminary results were presented by the PI in September 2004 and January 2005. We have also secured supporting optical observations of ES Ceti using the Magellan telescopes (November 2004). The team is currently performing a thorough and final analysis of the X-ray, UV and optical data sets with the latest XMM pipeline software and our own analysis packages.

  7. Department of Energy Plutonium ES&H Vulnerability Assessment Savannah River Site interim compensatory measures

    SciTech Connect

    Bickford, W.E.

    1994-09-15

    The Savannah River Site (SRS) has recently completed a self-assessment of potential vulnerabilities associated with plutonium and other transuranic materials stored at the site. An independent Working Group Assessment Team (WGAT) appointed by DOE/ES&H also performed an independent assessment, and reviewed and validated the site self-assessment. The purpose of this report is to provide a status of interim compensatory measures at SRS to address hazards in advance of any corrective actions. ES&H has requested this status for all vulnerabilities ranked medium or higher with respect to potential consequences to workers, environment, and the public.

  8. Pharmacokinetics of PEGylated recombinant human endostatin (M2ES) in rats

    PubMed Central

    Li, Zuo-gang; Jia, Lin; Guo, Li-fang; Yu, Min; Sun, Xu; Nie, Wen; Fu, Yan; Rao, Chun-ming; Wang, Jun-zhi; Luo, Yong-zhang

    2015-01-01

    Aim: M2ES is PEGylated recombinant human endostatin. In this study we investigated the pharmacokinetics, tissue distribution, and excretion of M2ES in rats. Methods: 125I-radiolabeled M2ES was administered to rats by intravenous bolus injection at 3 mg/kg. The pharmacokinetics, tissue distribution and excretion of M2ES were investigated using the trichloroacetic acid (TCA) precipitation method. Results: The serum M2ES concentration-time curve after a single intravenous dose of 3 mg/kg in rats was fitted with a non-compartment model. The pharmacokinetic parameters were evaluated as follows: Cmax=28.3 μg·equ/mL, t1/2=71.5 h, AUC(0–∞)=174.6 μg·equ·h/mL, Cl=17.2 mL·h−1·kg−1, MRT=57.6 h, and Vss=989.8 mL/kg for the total radioactivity; Cmax=30.3 μg·equ/mL, t1/2=60.1 h, AUC(0–∞)=146.2 μg·equ·h/mL, Cl=20.6 mL·h−1·kg−1, MRT=47.4 h, and Vss=974.6 mL/kg for the TCA precipitate radioactivity. M2ES was rapidly and widely distributed in various tissues and showed substantial deposition in kidney, adrenal gland, lung, spleen, bladder and liver. The radioactivity recovered in the urine and feces by 432 h post-dose was 71.3% and 8.3%, respectively. Only 0.98% of radioactivity was excreted in the bile by 24 h post-dose. Conclusion: PEG modification substantially prolongs the circulation time of recombinant human endostatin and effectively improves its pharmacokinetic behavior. M2ES is extensively distributed in most tissues of rats, including kidney, adrenal gland, lung, spleen, bladder and liver. Urinary excretion was the major elimination route for M2ES. PMID:26027657

  9. [Expression of retinoic acid receptor gamma gene in ES cells and its effect on their differentiation and apoptosis].

    PubMed

    Du, Z W; Tsung, H C; Yao, Z

    1998-06-01

    We have constructed pSG5-RAR gamma-neo plasmid containing mouse retinoic acid receptor gamma (RAR gamma) gene and neo gene, and introduced it into embryonic stem ES-5 cells, by calcium phosphate mediated transfection. Some G418-resistant clones were isolated and from RNA dot blot analysis of these clones, a clone overexpressing RAR gamma gene was established, designated as ES-gamma cell line. Northern blot hydridization and Southern blot hydridization analysis of ES-gamma cells (Fig 3, 4) demonstrated that ES-gamma cells overexpressed exogenous RAR gamma mRNA and the exogenous RAR gamma cDNA integrated into the genome of ES cells. ES-gamma cells retained undifferentiated morphology and positive alkaline phosphatase activity (Plate I, Fig. 1, 2), so it resembled ES-5 cells in terms of stem cell characteristics. When ES-gamma cells were subcutaneously inoculated into nude mouse and differentiated in vivo, tumorous nodules containing various tissue structures were obtained, demonstrating their pluripotent properties just like parent ES-5 cells. Contrasting with ES-5 cells, the histological features of tumors showed no cartilage tissues, but abundant muscle tissues and keratinized cyst like structures constituted by stratified squamous epithelia (Plate I, Fig. 3). Differentiating in vitro by hanging drop culture methods, ES-gamma cells differentiated mostly into fibroblast-like cells, (Plate II, Fig. 1-5). The above results indicated that overexpression of RAR gamma gene changed the cell type of ES cells differentiating in vivo and in vitro. During the differentiation of ES-5 cells induced by RA, a large number of cells rounded up, detached from the dish and tended to die. We suspected that this phenomenon may be apoptosis. The ultrastructure appearance of the dying cells displayed typical apoptotic changes including chromatin condensation and nuclear fragmentation (Plate I, Fig. 4, 5). Detection of DNA fragments using agarose gel electrophoresis showed characteristic

  10. Planetary transit observations at the University Observatory Jena: TrES-2

    NASA Astrophysics Data System (ADS)

    Raetz, St.; Mugrauer, M.; Schmidt, T. O. B.; Roell, T.; Eisenbeiss, T.; Hohle, M. M.; Koeltzsch, A.; Vaňko, M.; Ginski, Ch.; Marka, C.; Moualla, M.; Tetzlaff, N.; Seifahrt, A.; Broeg, Ch.; Koppenhoefer, J.; Raetz, M.; Neuhäuser, R.

    2009-05-01

    We report on observations of several transit events of the transiting planet TrES-2 obtained with the Cassegrain-Teleskop-Kamera at the University Observatory Jena. Between March 2007 and November 2008 ten different transits and almost a complete orbital period were observed. Overall, in 40 nights of observation 4291 exposures (in total 71.52 h of observation) of the TrES-2 parent star were taken. With the transit timings for TrES-2 from the 34 events published by the TrES-network, the Transit Light Curve project and the Exoplanet Transit Database plus our own ten transits, we find that the orbital period is P=(2.470614± 0.000001) d, a slight change by ˜ 0.6 s compared to the previously published period. We present new ephemeris for this transiting planet. Furthermore, we found a second dip after the transit which could either be due to a blended variable star or occultation of a second star or even an additional object in the system. Our observations will be useful for future investigations of timing variations caused by additional perturbing planets and/or stellar spots and/or moons. Based on observations obtained with telescopes of the University Observatory Jena, which is operated by the Astrophysical Institute of the Friedrich-Schiller-University Jena and the 80cm telescope of the Wendelstein Observatory of the Ludwig-Maximilians-University Munich.

  11. Enterprise Systems (ES) Software in Business School Curriculum--Evaluation of Design and Delivery

    ERIC Educational Resources Information Center

    Seethamraju, Ravi

    2007-01-01

    Considering the increasing importance of enterprise systems in business, and their pedagogical value in demonstrating business process orientation and concepts of integration, several universities have incorporated popular enterprise system (ES) software products such as SAP R/3 into their business school curricula. This paper describes an attempt…

  12. BMP4 and FGF strongly induce differentiation of mouse ES cells into oral ectoderm.

    PubMed

    Ochiai, Hiroshi; Suga, Hidetaka; Yamada, Tomiko; Sakakibara, Mayu; Kasai, Takatoshi; Ozone, Chikafumi; Ogawa, Koichiro; Goto, Motomitsu; Banno, Ryoichi; Tsunekawa, Shin; Sugimura, Yoshihisa; Arima, Hiroshi; Oiso, Yutaka

    2015-09-01

    During embryonic development, oral ectoderm differentiates into the adenohypophysis, dental epithelia, salivary glands, and nasal pit. Few reports exist concerning the induction of oral ectoderm from embryonic stem (ES) cells. Generally, any lot differences in fetal bovine serum (FBS) and serum replacer may affect the induction of ES cell-differentiation. Using a previously established culture strategy for differentiation, the proportion of cell aggregates containing Pitx1+ oral ectoderm varied widely between 9-36% when several different lots of FBS or serum replacer were used. We therefore tried to enhance the differentiation method. We found that bone morphogenetic protein (BMP) 4 and fibroblast growth factor (FGF) treatments improved oral ectoderm induction. Such treatment also improved the differentiation of oral ectoderm into the adenohypophysis. Furthermore, increased BMP4 treatment induced dental epithelium and mesenchyme. Such differentiation suggests that the Pitx1+ layer displays similar properties to oral ectoderm, as found in vivo. Differentiation of ES cells into oral ectoderm using different lots of FBS and serum replacer increased 78-90% after treatment with BMP4 and FGF. In summary, we have established a robust strategy for the induction of oral ectoderm differentiation from mouse ES cells. PMID:26209816

  13. Draft Genome Sequence of Se(IV)-Reducing Bacterium Pseudomonas migulae ES3-33

    PubMed Central

    Li, Xuanji; Kot, Witold; Wang, Dan; Zheng, Shixue; Wang, Gejiao; Hansen, Lars H.

    2015-01-01

    Pseudomonas migulae ES3-33 is a Gram-negative strain that strongly reduces Se(IV) and was isolated from a selenium mining area in Enshi, southwest China. Here we present the draft genome of this strain containing potential genes involved in selenite reduction and a large number of genes encoding resistances to copper and antibiotics. PMID:25953191

  14. How Children Living in Poor Areas of Dar Es Salaam, Tanzania Perceive Their Own Multiple Intelligences

    ERIC Educational Resources Information Center

    Dixon, Pauline; Humble, Steve; Chan, David W.

    2016-01-01

    This study was carried out with 1,857 poor children from 17 schools, living in low-income areas of Dar Es Salaam, Tanzania. All children took the "Student Multiple Intelligences Profile" (SMIP) questionnaire as part of a bigger project that gathered data around concepts and beliefs of talent. This paper sets out two aims, first to…

  15. Distance Learning Methodologies. TRANSCOM Regulating and Command & Control Evacuation System (TRAC2ES).

    ERIC Educational Resources Information Center

    Bloomquist, Carroll R.

    The TRANSCOM (Transportation Command) Regulating Command and Control Evacuation System (TRAC2ES), which applies state-of-the-art technology to manage global medical regulating (matching patients to clinical availability) and medical evacuation processes, will be installed at all Department of Defense medical locations globally. A combination of…

  16. Child Labour in Urban Agriculture: The Case of Dar es Salaam, Tanzania.

    ERIC Educational Resources Information Center

    Mlozi, Malongo R. S.

    1995-01-01

    Urban agriculture in Dar es Salaam was found to use child labor of both children with parents of higher and lower socioeconomic status (SES). Discusses policy implications and calls for the education of parents of lower SES not to expect an economic contribution from their children's labor, and the education of children about their rights. (LZ)

  17. Single-molecule observation of protein folding in symmetric GroEL-(GroES)2 complexes.

    PubMed

    Takei, Yodai; Iizuka, Ryo; Ueno, Taro; Funatsu, Takashi

    2012-11-30

    The chaperonin, GroEL, is an essential molecular chaperone that mediates protein folding together with its cofactor, GroES, in Escherichia coli. It is widely believed that the two rings of GroEL alternate between the folding active state coupled to GroES binding during the reaction cycle. In other words, an asymmetric GroEL-GroES complex (the bullet-shaped complex) is formed throughout the cycle, whereas a symmetric GroEL-(GroES)(2) complex (the football-shaped complex) is not formed. We have recently shown that the football-shaped complex coexists with the bullet-shaped complex during the reaction cycle. However, how protein folding proceeds in the football-shaped complex remains poorly understood. Here, we used GFP as a substrate to visualize protein folding in the football-shaped complex by single-molecule fluorescence techniques. We directly showed that GFP folding occurs in both rings of the football-shaped complex. Remarkably, the folding was a sequential two-step reaction, and the kinetics were in excellent agreement with those in the bullet-shaped complex. These results demonstrate that the same reactions take place independently in both rings of the football-shaped complex to facilitate protein folding. PMID:23048033

  18. AGILE confirmation of enhanced gamma-ray activity from the Blazar 1ES 1959+650

    NASA Astrophysics Data System (ADS)

    Lucarelli, F.; Pittori, C.; Verrecchia, F.; Bulgarelli, A.; Fioretti, V.; Zoli, A.; Piano, G.; Munar-Adrover, P.; Tavani, M.; Donnarumma, I.; Vercellone, S.; Striani, E.; Cardillo, M.; Gianotti, F.; Trifoglio, M.; Giuliani, A.; Mereghetti, S.; Caraveo, P.; Perotti, F.; Chen, A.; Argan, A.; Costa, E.; Del Monte, E.; Evangelista, Y.; Feroci, M.; Lazzarotto, F.; Lapshov, I.; Pacciani, L.; Soffitta, P.; Sabatini, S.; Vittorini, V.; Pucella, G.; Rapisarda, M.; Di Cocco, G.; Fuschino, F.; Galli, M.; Labanti, C.; Marisaldi, M.; Pellizzoni, A.; Pilia, M.; Trois, A.; Barbiellini, G.; Vallazza, E.; Longo, F.; Morselli, A.; Picozza, P.; Prest, M.; Lipari, P.; Zanello, D.; Cattaneo, P. W.; Rappoldi, A.; Colafrancesco, S.; Parmiggiani, N.; Ferrari, A.; Antonelli, A.; Giommi, P.; Salotti, L.; Valentini, G.; D'Amico, F.

    2016-06-01

    Following ATel #9148, reporting multi-wavelength activity from the BL Lac type blazar 1ES 1959+650, AGILE also detects increased gamma-ray emission above 100 MeV from a position compatible with this BL Lac source.

  19. Integrating ICT into Teaching and Learning at the University of Dar es Salaam

    ERIC Educational Resources Information Center

    Mtebe, Joel S.; Dachi, Hilary; Raphael, Christina

    2011-01-01

    Since 1985, Tanzania has been undergoing significant political and economic changes from a centralized to a more market-oriented and globally connected economy. The University of Dar es Salaam (UDSM) has responded to these changes by reviewing its legal status, vision, and functions, particularly those related to research, teaching, and public…

  20. Instantánea del cáncer de esófago

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer de esófago; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  1. A Conceptual Critique of the EA:es Comparison in the Comprehensive Rorschach System.

    ERIC Educational Resources Information Center

    Kleiger, James H.; Exner, John E., Jr.

    1992-01-01

    The EA:es index (part of the Comprehensive Rorschach System) is discussed, demonstrating how conceptual difficulties and abstract jargon can result in misleading and contradictory inferences. The comments of J. E. Exner, Jr., and the response of J. H. Kleiger concern the importance of theory in Rorschach interpretation. (SLD)

  2. Students' Experiences and Challenges of Blended Learning at the University of Dar Es Salaam, Tanzania

    ERIC Educational Resources Information Center

    Mtebe, Joel S.; Raphael, Christina

    2013-01-01

    Recent developments in Information and Communication Technologies (ICTs), especially eLearning, have heightened the need for University of Dar es Salaam (UDSM) to supplement on-campus face-to-face delivery as well as meeting increased students' enrolments through blended distance learning. Since 2008, the University has been offering three…

  3. BMP4 and FGF strongly induce differentiation of mouse ES cells into oral ectoderm.

    PubMed

    Ochiai, Hiroshi; Suga, Hidetaka; Yamada, Tomiko; Sakakibara, Mayu; Kasai, Takatoshi; Ozone, Chikafumi; Ogawa, Koichiro; Goto, Motomitsu; Banno, Ryoichi; Tsunekawa, Shin; Sugimura, Yoshihisa; Arima, Hiroshi; Oiso, Yutaka

    2015-09-01

    During embryonic development, oral ectoderm differentiates into the adenohypophysis, dental epithelia, salivary glands, and nasal pit. Few reports exist concerning the induction of oral ectoderm from embryonic stem (ES) cells. Generally, any lot differences in fetal bovine serum (FBS) and serum replacer may affect the induction of ES cell-differentiation. Using a previously established culture strategy for differentiation, the proportion of cell aggregates containing Pitx1+ oral ectoderm varied widely between 9-36% when several different lots of FBS or serum replacer were used. We therefore tried to enhance the differentiation method. We found that bone morphogenetic protein (BMP) 4 and fibroblast growth factor (FGF) treatments improved oral ectoderm induction. Such treatment also improved the differentiation of oral ectoderm into the adenohypophysis. Furthermore, increased BMP4 treatment induced dental epithelium and mesenchyme. Such differentiation suggests that the Pitx1+ layer displays similar properties to oral ectoderm, as found in vivo. Differentiation of ES cells into oral ectoderm using different lots of FBS and serum replacer increased 78-90% after treatment with BMP4 and FGF. In summary, we have established a robust strategy for the induction of oral ectoderm differentiation from mouse ES cells.

  4. 75 FR 4759 - Polychlorinated Biphenyls: Manufacturing (Import) Exemption for Veolia ES Technical Solutions, LLC

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-29

    ..., LLC, which was published in the Federal Register on March 6, 2008 (73 FR 12053). In addition to... Federal Register notice for the hearing (73 FR 28786, May 19, 2008). In addition to all of the pre... Veolia ES Technical Solutions, LLC AGENCY: Environmental Protection Agency (EPA). ACTION: Withdrawal...

  5. The NCTM Process Standards and the Five "Es" of Science: Connecting Math and Science

    ERIC Educational Resources Information Center

    Bosse, Michael J.; Lee, Tammy D.; Swinson, Michael; Faulconer, Johna

    2010-01-01

    This study investigates defining characteristics among the process standards of the Principles and Standards for School Mathematics and the 5 "Es" from the National Science Education Standards and the Inquiry and the National Science Education Standards. These characteristics are used to demonstrate similarities and differences between the…

  6. Clinical, Virologic, and Epidemiologic Characteristics of Dengue Outbreak, Dar es Salaam, Tanzania, 2014

    PubMed Central

    Mboera, Leonard E.G.; De Nardo, Pasquale; Oriyo, Ndekya M.; Meschi, Silvia; Rumisha, Susan F.; Colavita, Francesca; Mhina, Athanas; Carletti, Fabrizio; Mwakapeje, Elibariki; Capobianchi, Maria Rosaria; Castilletti, Concetta; Di Caro, Antonino; Nicastri, Emanuele; Malecela, Mwelecele N.; Ippolito, Giuseppe

    2016-01-01

    We investigated a dengue outbreak in Dar es Salaam, Tanzania, in 2014, that was caused by dengue virus (DENV) serotype 2. DENV infection was present in 101 (20.9%) of 483 patients. Patient age and location of residence were associated with infection. Seven (4.0%) of 176 patients were co-infected with malaria and DENV. PMID:27088845

  7. 29ièmes Journées Franco-Belges de Pharmacochimie: Meeting Report

    PubMed Central

    2015-01-01

    The “Journées Franco-Belges de Pharmacochimie” is a recognized two-day annual meeting on Medicinal Chemistry that is renowned for the advanced science presented, conviviality, and outstanding opportunities for senior and young scientists to exchange knowledge. Abstracts of plenary lectures, oral communications, and posters presented during the meeting are collected in this report. PMID:26593925

  8. EphB4 Forward-Signaling Regulates Cardiac Progenitor Development in Mouse ES Cells

    PubMed Central

    Liu, Yanfeng; Hoyle, Dixie L.; Shen, Wei-Feng; Wu, Li-Qun; Wang, Zack Z.

    2015-01-01

    Eph receptor (Eph)-ephrin signaling plays an important role in organ development and tissue regeneration. Bidirectional signaling of EphB4– ephrinB2 regulates cardiovascular development. To assess the role of EphB4–ephrinB2 signaling in cardiac lineage development, we utilized two GFP reporter systems in embryonic stem (ES) cells, in which the GFP transgenes were expressed in Nkx2.5+ cardiac progenitor cells and in α-MHC+ cardiomyocytes, respectively. We found that both EphB4 and ephrinB2 were expressed in Nkx2.5-GFP+ cardiac progenitor cells, but not in α-MHC-GFP+ cardiomyocytes during cardiac lineage differentiation of ES cells. An antagonist of EphB4, TNYL-RAW peptides, that block the binding of EphB4 and ephrinB2, impaired cardiac lineage development in ES cells. Inhibition of EphB4–ephrinB2 signaling at different time points during ES cell differentiation demonstrated that the interaction of EphB4 and ephrinB2 was required for the early stage of cardiac lineage development. Forced expression of human full-length EphB4 or intracellular domain-truncated EphB4 in EphB4-null ES cells was established to investigate the role of EphB4-forward signaling in ES cells. Interestingly, while full-length EphB4 was able to restore the cardiac lineage development in EphB4-null ES cells, the truncated EphB4 that lacks the intracellular domain of tyrosine kinase and PDZ motif failed to rescue the defect of cardiomyocyte development, suggesting that EphB4 intracellular domain is essential for the development of cardiomyocytes. Our study provides evidence that receptor-kinase-dependent EphB4-forward signaling plays a crucial role in the development of cardiac progenitor cells. PMID:25359705

  9. Modelling present and future African climate using CMIP5scenarios in HadGEM2-ES

    NASA Astrophysics Data System (ADS)

    Shimizu, M. H.; Diallo, M.; Dike, V. N.

    2014-12-01

    The present precipitation and temperature patterns and expected future changes (2073-2098) in Africa are investigated using the Hadley Centre Global Environmental Model 2-Earth System (HadGEM2-ES) under the fifth phase of the Coupled Model Intercomparison Project (CMIP5) protocols for historical and future emission scenarios simulations.In a CMIP5 multimodel analysis, the annual cycles of temperature and precipitation simulated by HadGEM2-ES were very close to the multimodel ensemble mean. HadGEM2-ES temperature simulation compares well with the National Center for Atmospheric Research (NCAR) reanalysis over the 1979-2004 periods, except for a summer overestimation in Central Africa, and a winter underestimation in tropical West Africa. The precipitation simulation compared well with the Global Precipitation Climatology Project (GPCP) data from 1979 to 2004 over the entire Africa, except in the Intertropical Convergence Zone (ITCZ), where the model fails to capture adequately the transition phase of the monsoon circulation. The dry regimes over Northern Africa as well as the wetter regime occurring over Central Africa, which is mainly regulated by the ITCZ displacement, and during the austral summer of Southern Africa, are also fairly reproduced by the HadGEM2-ES model. The model projects for the end of the 21st century a rainy South Africa, a change of the flood/drought cycle in the Tropics and a warming over the whole continent, varying from 3 to 7 ∘ C. HadGEM2-ES performance for Nigeria shows good reproduction of precipitation seasonal cycles for some locations, outside the ITCZ. However, the comparison with in situ measurement in Ilorin and Lagos shows the model is not being able to reproduce the precipitation annual cycle. Future projections for Nigeria exhibit warming everywhere and an enhancement of precipitation, especially in the northern part of the country.

  10. Cloning mice and ES cells by nuclear transfer from somatic stem cells and fully differentiated cells.

    PubMed

    Wang, Zhongde

    2011-01-01

    Cloning animals by nuclear transfer (NT) has been successful in several mammalian species. In addition to cloning live animals (reproductive cloning), this technique has also been used in several species to establish cloned embryonic stem (ntES) cell lines from somatic cells. It is the latter application of this technique that has been heralded as being the potential means to produce isogenic embryonic stem cells from patients for cell therapy (therapeutic cloning). These two types of cloning differ only in the steps after cloned embryos are produced: for reproductive cloning the cloned embryos are transferred to surrogate mothers to allow them to develop to full term and for therapeutic cloning the cloned embryos are used to derive ntES cells. In this chapter, a detailed NT protocol in mouse by using somatic stem cells (neuron and skin stem cells) and fully differentiated somatic cells (cumulus cells and fibroblast cells) as nuclear donors is described.

  11. Flight Test Results of VDL-3, 1090ES, and UAT Datalinks for Weather Information Communication

    NASA Technical Reports Server (NTRS)

    Griner, James

    2006-01-01

    This presentation describes final test results for the Weather Information Communications (WINCOMM) program at the NASA Glenn Research Center on flight testing of the 1090 Extended Squitter (1090ES), VDL Mode 3, and Universal Access Transceiver (UAT) data links as a medium for weather data exchange. It presents an architectural description of the use of 1090ES to meet the program objectives of sending turbulence information, the use of VDL Mode 3 to send graphical weather images, and the use of UAT for transmitting weather sensor data. This presentation provides a high level definition of the changes made to both avionics and ground-based receivers as well as the ground infrastructure used to support flight testing and future implementation. Summary of results from flight tests of these datalinks will also be presented.

  12. [Practical features of the pre-analytical phase of the BIOAMBIENT.ES biomonitoring study].

    PubMed

    Esteban, Marta; Ruiz-Moraga, Montserrat; Pérez-Gómez, Beatriz; Castaño, Argelia

    2013-01-01

    The fieldwork of BIOAMBIENT.ES was developed from March 2009 to July 2010. BIOAMBIENT.ES is a human biomonitoring study of environmental pollutants performed in Spain at the national level. This article aims to show the tasks performed before starting the fieldwork to ensure the quality of the samples and consequently the quality of the results. A total of 1,936 whole blood, serum and first-morning urine samples and 604 hair samples were collected from workers who attended the annual occupational health examination in 38 centers in the Peninsula, Ceuta and the Canary Islands. Before the fieldwork was started, the optimal sampling material and sample shipment was identified and fieldworkers were trained in their tasks. Due to the planning and organization of the pre-analytical phase, only 1% of the collected samples had to be rejected due to problem with spills, conservation, etc. In addition, the analyses conducted showed no pre-analytical interferences.

  13. Actinide cross sections from the reaction of sup 13 C with sup 254 Es sup g

    SciTech Connect

    Moody, K.J.; Lougheed, R.W.; Dougan, R.J.; Hulet, E.K.; Wild, J.F.; Summerer, K.; Hahn, R.L.; Aarle, J.v.; Bethune, G.R. )

    1990-01-01

    We have measured cross sections for the formation of actinide transfer products in the reaction of 72-MeV {sup 13}C projectiles with {sup 254}Es{sup {ital g}} targets. The pattern of nuclide yields is similar to those observed in the reactions of heavier ions with {sup 254}Es{sup {ital g}}. We have constructed the primary element yields from these results and show that the total cross section for transfer reactions is 58 mb. The total reaction cross section is about 300 mb. Lawrencium isotopes are formed with larger cross sections than are consistent with the trends of the transfer-product distributions; we explain this in terms of massive transfer, and model the lawrencium yields with an evaporation code.

  14. Analysis of the (1, lambda)-ES on the parabolic ridge.

    PubMed

    Oyman, A I; Beyer, H G; Schwefel, H P

    2000-01-01

    The progress rate of the (1,+ lambda)-ES (Evolution Strategy) is analyzed on the parabolic ridge test function. A different progress behavior is observed for the (1, lambda)-ES than for the sphere model test function. The characteristics of the progress rate picture for the plus strategy differs little from the one obtained for the sphere model, but this strategy has drastically worse progress rate values than those obtained for the comma strategy. The dynamics of the distance to the progress axis is also investigated. A theoretical formula is derived to estimate the change in this distance over generations. This formula is used to derive the expected value of the problem-specific distance to the ridge axis. The correctness of the formulae is supported by simulation results.

  15. Analysis of the (mu/mu, lambda)-ES on the parabolic ridge.

    PubMed

    Oyman, A I; Beyer, H G

    2000-01-01

    The progress behavior of evolution strategies (ES) using recombination is analyzed in this paper on the parabolic ridge. This test function represents landscapes far from the optimum. The ES algorithms with intermediate and dominant recombination are considered in the analysis. The derivations are presented for intermediate recombination. Thereafter, the formulae for dominant recombination are obtained using the so-called surrogate mutation model. In the analysis, the formulae are derived for the progress rate psi and for the stationary distance R(infinity) to the ridge axis. As a result, it will be shown that the progress rate psi can increase if recombination is applied. Simulations are used to show the appropriateness of the formulae derived.

  16. Optical and Near-UV Observations of the Transiting Extrasolar Planet TrES-4b

    NASA Astrophysics Data System (ADS)

    Smith, Carter-Thaxton; Turner, J.; Carleton, T.; Crawford, B.; Guvenen, B.; Hardegree-Ullman, K.; Small, L.; Towner, A. P.; Walker-LaFollette, A.; Henz, T.

    2013-01-01

    Using the Steward Observatory 61” Kuiper Telescope, The University of Arizona Astronomy Club conducted photometric observations of the transiting extrasolar planet TrES-4b as part of the Exoplanet Observation Project. Observations were made in the Bessell U, Harris B, and Harris R filters. Initial observations were made in 2009, with follow up observations in 2011. Basic data reduction and photometry was done using IRAF and determination of transit parameters was done using Transit Analysis Package (TAP) and JKTEBOP transit modeling code. We present an updated planetary mass, radius, density, surface gravity, Safronov number, equilibrium temperature, orbital distance, and orbital inclination for TrES-4b. In addition, we also searched for asymmetries between the near-UV and optical light curves. This project, started in spring 2009, has introduced many undergraduate students to research and given them valuable experience with data reduction and observation techniques.

  17. A strong X-ray Flare in 1ES 1959+650

    NASA Astrophysics Data System (ADS)

    Kapanadze, Bidzina

    2016-06-01

    The nearby TeV-detected HBL object 1ES 1959+650 (z=0.047) has been observed by Swift today which revealed a strong X-ray flare in the source. Namely, the observation-binned 0.3-10 keV count rate is 16.49+/-0.15 cts/s that is by a factor 2.45 larger compared to weighted mean count rate from all Swift-XRT pointings to this source, and by 90% larger than the rate recorded during the previous observation (performed on June 4). Note that the higher brightness states were observed only three times in the past (in 2015 September - December; see Kapanadze B. et al. 2016, "A recent strong X-ray flaring activity of 1ES 1959+650 with possibly less efficient stochastic acceleration", MNRASL, in press).

  18. Ultratraces of carotenes in tomato purées: HPLC-TLS study

    NASA Astrophysics Data System (ADS)

    Luterotti, S.; Marković, K.; Franko, M.; Bicanic, D.; Vahčić, N.; Doka, O.

    2003-01-01

    The present study was designed to provide information about (i) the profile of carotene pigments and (ii) trace quantities of lycopene and β-carotene left in tomato purées. The ultrasensitive method comprising HPLC and thermal lens spectrometric (TLS) detection enabled us to detect as low as 0.3 and 1.1 ng ml-1 lycopene and β-carotene in purée extracts, respectively. Total concentration of β-carotene and lycopene (varying from 3 to 170 ng g-1) in the examined tomato purées may serve as an indicator of the carotene-specific antioxidative capacity of these products. Although conventional spectrophotometry can be used to rapidly assess the quality of products derived from tomatoes, a highly sensitive and selective method such as HPLC-TLS is needed for reliable analyses of samples such as, for example, those subjected to inappropriate storage and/or handling.

  19. Furan formation during storage and reheating of sterilised vegetable purées.

    PubMed

    Palmers, Stijn; Grauwet, Tara; Buvé, Carolien; Van de Vondel, Lore; Kebede, Biniam T; Hendrickx, Marc E; Van Loey, Ann

    2015-01-01

    To this day, research for furan mitigation has mostly targeted the levels of food production and handling of prepared foods by the consumer. However, part of the furan concentrations found in commercially available food products might originate from chemical deterioration reactions during storage. A range of individual vegetable purées was stored at two different temperatures to investigate the effects of storage on the furan concentrations of shelf-stable, vegetable-based foods. After 5 months of storage at 35°C (temperature-abuse conditions), a general increase in furan concentrations was observed. The furan formation during storage could be reduced by storing the vegetable purées at a refrigerated temperature of 4°C, at which the furan concentrations remained approximately constant for at least 5 months. Following storage, the vegetable purées were briefly reheated to 90°C to simulate the effect of the final preparation step before consumption. Contrary to storage, furan concentrations decreased as a result of evaporative losses. Both refrigerated storage and the reheating step prior to consumption showed the potential of mitigation measures for furan formation in vegetable-based foods (e.g. canned vegetables, ready-to-eat soups, sauces or baby foods). Next to furan, the vegetable purées were analysed for 2- and 3-methylfuran. Tomato was very susceptible to the formation of both alkylated derivatives of furan, as opposed to the other vegetables in this study. Methylfuran concentrations rapidly decreased during storage, which was contrary to the results observed for furan. PMID:25522980

  20. Cáncer de esófago—Versión para profesionales de salud

    Cancer.gov

    Información del Instituto Nacional del Cáncer sobre el tratamiento, la prevención y los exámenes de detección del cáncer de esófago, así como referencias a estudios clínicos, investigación, estadísticas y otros temas relacionados con este tipo de cáncer.

  1. Cáncer de esófago—Versión para pacientes

    Cancer.gov

    Información del Instituto Nacional del Cáncer sobre el tratamiento, la prevención y los exámenes de detección del cáncer de esófago, así como referencias a estudios clínicos, investigación, estadísticas y otros temas relacionados con este tipo de cáncer.

  2. Further increase of gamma-ray emission from the HBL 1ES 1959+650

    NASA Astrophysics Data System (ADS)

    Biland, A.; Dorner, D.; Mirzoyan, R.; Mukherjee, R.; Buson, S.; Kapanazde, B.

    2016-06-01

    FACT, MAGIC, VERITAS and Fermi-LAT collaborations report the measurement of a further increase of the gamma-ray flux together with bright X-ray emission seen by Swift-XRT from a position consistent with the high-energy peaked BL Lac type object 1ES 1959+650 (z=0.047, Schachter et al. 1993, ApJ, 412, 541).

  3. Detection of Planetary Emission from the Exoplanet TrES-2 Using Spitzer/IRAC

    NASA Technical Reports Server (NTRS)

    Donovan, Francis T.; Charbonneau, David; Harrington, Joseph; Madhusudhan, N.; Seager, Sara; Deming, Drake; Knutson, Heather A.

    2010-01-01

    We present here the results of our observations of TrES-2 using the Infrared Array Camera on Spitzer. We monitored this transiting system during two secondary eclipses, when the planetary emission is blocked by the star. The resulting decrease in flux is 0.127% +/- 0.021%, 0.230% +/- 0.024%, 0.199% +/- 0.054%, and 0.359% +/- 0.060% at 3.6 microns, 4.5 microns, 5.8 microns, and 8.0 microns, respectively. We show that three of these flux contrasts are well fit by a blackbody spectrum with T(sub eff) = 1500 K, as well as by a more detailed model spectrum of a planetary atmosphere. The observed planet-to-star flux ratios in all four lRAC channels can be explained by models with and without a thermal inversion in the atmosphere of TrES-2, although with different atmospheric chemistry. Based on the assumption of thermochemical equilibrium, the chemical composition of the inversion model seems more plausible, making it a more favorable scenario. TrES-2 also falls in the category of highly irradiated planets which have been theoretically predicted to exhibit thermal inversions. However, more observations at infrared and visible wavelengths would be needed to confirm a thermal inversion in this system. Furthermore, we find that the times of the secondary eclipses are consistent with previously published times of transit and the expectation from a circular orbit. This implies that TrES-2 most likely has a circular orbit, and thus does not obtain additional thermal energy from tidal dissipation of a non-zero orbital eccentricity, a proposed explanation for the large radius of this planet. Key words: eclipses - infrared: stars - planetary systems - stars: individual (OSC 03549-02811) - techniques: photometric

  4. DETECTION OF PLANETARY EMISSION FROM THE EXOPLANET TrES-2 USING SPITZER/IRAC

    SciTech Connect

    O'Donovan, Francis T.; Charbonneau, David; Knutson, Heather A.; Harrington, Joseph; Madhusudhan, N.; Seager, Sara; Deming, Drake

    2010-02-20

    We present here the results of our observations of TrES-2 using the Infrared Array Camera on Spitzer. We monitored this transiting system during two secondary eclipses, when the planetary emission is blocked by the star. The resulting decrease in flux is 0.127% +- 0.021%, 0.230% +- 0.024%, 0.199% +- 0.054%, and 0.359% +- 0.060% at 3.6 {mu}m, 4.5 {mu}m, 5.8 {mu}m, and 8.0 {mu}m, respectively. We show that three of these flux contrasts are well fit by a blackbody spectrum with T{sub eff} = 1500 K, as well as by a more detailed model spectrum of a planetary atmosphere. The observed planet-to-star flux ratios in all four IRAC channels can be explained by models with and without a thermal inversion in the atmosphere of TrES-2, although with different atmospheric chemistry. Based on the assumption of thermochemical equilibrium, the chemical composition of the inversion model seems more plausible, making it a more favorable scenario. TrES-2 also falls in the category of highly irradiated planets which have been theoretically predicted to exhibit thermal inversions. However, more observations at infrared and visible wavelengths would be needed to confirm a thermal inversion in this system. Furthermore, we find that the times of the secondary eclipses are consistent with previously published times of transit and the expectation from a circular orbit. This implies that TrES-2 most likely has a circular orbit, and thus does not obtain additional thermal energy from tidal dissipation of a non-zero orbital eccentricity, a proposed explanation for the large radius of this planet.

  5. La grossesse chez les hémodialysées chroniques

    PubMed Central

    Doukkali, Bouchra; Bahadi, Abdelaali; Rafik, Hicham; Kabbaj, Driss; Benyahia, Mohamed

    2015-01-01

    La survenue d'une grossesse en hémodialyse chronique (HDC) est rare, mais depuis la description du premier cas par Confortini en 1971, plusieurs observations ont été rapportées. L'hémodialyse a considérablement amélioré la fertilité de ces patientes. Nous rapportons l'expérience de douze grossesses survenues entre 1999 et 2014, chez douze patientes d’âge médian 34 ans (22-44), en hémodialyse (HD) depuis 40 mois (3-72), l’âge gestationnel moyen de diagnostic est de 16 semaines d'aménorrhée, la grossesse était compliquée dans 50% des cas par un hydramnios. Le terme moyen est de 35 semaine d'aménorrhée (SA) et l'accouchement a été réalisé dans 90% des grossesses par voie basse. Le poids moyen des nouveau-nés est de 1800g. De telles grossesses sont à haut risque du fait de la fréquence des complications. Elles devraient être contrôlées par les équipes multidisciplinaires, et la consultation prénatal ne devrait pas être négligée. L'objectif de ce travail est de rapporter notre expérience concernant la survenue d'une grossesse chez les patientes dialysées et de la confronter aux données de la littérature. PMID:26113944

  6. Furan formation during storage and reheating of sterilised vegetable purées.

    PubMed

    Palmers, Stijn; Grauwet, Tara; Buvé, Carolien; Van de Vondel, Lore; Kebede, Biniam T; Hendrickx, Marc E; Van Loey, Ann

    2015-01-01

    To this day, research for furan mitigation has mostly targeted the levels of food production and handling of prepared foods by the consumer. However, part of the furan concentrations found in commercially available food products might originate from chemical deterioration reactions during storage. A range of individual vegetable purées was stored at two different temperatures to investigate the effects of storage on the furan concentrations of shelf-stable, vegetable-based foods. After 5 months of storage at 35°C (temperature-abuse conditions), a general increase in furan concentrations was observed. The furan formation during storage could be reduced by storing the vegetable purées at a refrigerated temperature of 4°C, at which the furan concentrations remained approximately constant for at least 5 months. Following storage, the vegetable purées were briefly reheated to 90°C to simulate the effect of the final preparation step before consumption. Contrary to storage, furan concentrations decreased as a result of evaporative losses. Both refrigerated storage and the reheating step prior to consumption showed the potential of mitigation measures for furan formation in vegetable-based foods (e.g. canned vegetables, ready-to-eat soups, sauces or baby foods). Next to furan, the vegetable purées were analysed for 2- and 3-methylfuran. Tomato was very susceptible to the formation of both alkylated derivatives of furan, as opposed to the other vegetables in this study. Methylfuran concentrations rapidly decreased during storage, which was contrary to the results observed for furan.

  7. Coupling GSM/ALE with ES-FEM-T3 for fluid-deformable structure interactions

    NASA Astrophysics Data System (ADS)

    Wang, S.; Khoo, B. C.; Liu, G. R.; Xu, G. X.; Chen, L.

    2014-11-01

    In light of the effectiveness of the edge-based smoothed finite element method (ES-FEM-T3) and arbitrary Lagrangian-Eulerian gradient smoothing method (GSM/ALE) in, respectively, solving the pure solid and fluid flow problems using three-node triangular elements, they are coupled together in the present study to solve the more challenging fluid-deformable structure interaction (FSI) problems based on the weak coupling algorithm. Specifically, the fluid flow is tracked over the moving mesh with the well developed GSM/ALE and the transient response of the solid part is solved by the newly developed explicit ES-FEM-T3. The solutions from these two parts are “linked” together by the carefully formulated FSI coupling conditions on the FSI interface. Detailed procedures are summarized to illustrate the implementations of the GSM/ALE with ES-FEM-T3 in an FSI analysis. Three benchmarks are employed to validate the proposed coupled smoothed method in solving both transient and steady-state FSI problems. The mesh sensitivity analysis is further carried out showing that the results of an FSI system appear more sensitive to the change in the solid mesh as compared to the fluid mesh, thus suggesting a more refined mesh for the solid part. Another significant finding is that the present method can still produce reliable results even on the extremely distorted mesh near the FSI interface. The successful coupling GSM/ALE with ES-FEM-T3 for solving FSI problems serves as a good start for further implementing the family of smoothed methods in solving more complex cross-area problems.

  8. Multiple Mechanisms of Uranium Immobilization by Cellulomonas sp. strain ES6

    SciTech Connect

    Sivaswamy, Vaideeswaran; Brent Peyton; Viamajala, Sridhar; Robin Gerlach; William Apel; Rajesh Sani; Alice Dohnalkova; Thomas Borch

    2011-02-01

    Removal of hexavalent uranium (U(VI)) from aqueous solution was studied using a Gram-positive facultative anaerobe, Cellulomonas sp. strain ES6, under anaerobic, non growth conditions in bicarbonate and PIPES buffers. Inorganic phosphate was released by cells during the experiments providing ligands for formation of insoluble U(VI) phosphates. Phosphate release was most probably the result of anaerobic hydrolysis of intracellular polyphosphates accumulated by ES6 during aerobic growth. Microbial reduction of U(VI) to U(IV) was also observed. However, the relative magnitudes of U(VI) removal by abiotic (phosphate-based) precipitation and microbial reduction depended on the buffer chemistry. In bicarbonate buffer, X-ray absorption near edge structure (XANES) analysis showed U precipitates containing nearly equal fractions of U(IV) and U(VI), whereas in PIPES buffer, U precipitates consisted primarily of U(VI). Mass balance calculations for U and P corroborate these observations. High-resolution transmission electron microscopy (HR42TEM) and energy dispersive X-ray spectroscopy (EDS) showed both extracellular and intracellular accumulation of U solids. The U(VI)-phosphate precipitates, confirmed by EDS as containing U and P in equimolar concentrations, had nanometer sized lath structure. When anthraquinone-2,6-disulfonate (AQDS), a known electron shuttle, was added to the experimental reactors, U reduction became the dominant removal mechanism, in contrast to primarily phosphate-mediated precipitation observed in the absence of AQDS. Uranium immobilization by abiotic precipitation or microbial reduction has been extensively reported; however, present work suggests that strain ES6 can remove U(VI) from solution simultaneously through precipitation with phosphate ligands and microbial reduction, depending on the environmental conditions. Cellulomonadaceae are environmentally relevant subsurface bacteria and here, for the first time, t 52 he presence of multiple U

  9. [High-intensity chemotherapy versus palliative chemotherapy in patients over 60 years with acute myeloid leukemia].

    PubMed

    Medrano-Contreras, Jesús; Talavera-Piña, Juan Osvaldo; Guerrero-Rivera, Susana; Gutiérrez-Espíndola, Guillermo Rodolfo; Gómez-Cortés, Cynthia; Pérez-Rocha, Juan Fernando; Terreros-Muñoz, Eduardo; Meillón-García, Luis Antonio

    2016-01-01

    Introducción: el tratamiento con quimioterapia intensa (QTI) en pacientes con leucemia mieloblástica (LMA) mayores de 60 años es controversial. En el presente estudio se evaluó la remisión completa y la supervivencia global de pacientes con LMA mayores de 60 años, tratados con QTI o quimioterapia paliativa. Métodos: los pacientes con adecuada función orgánica y ECOG ≤ 2 recibieron QTI a base de citarabina por cinco o siete días más un antracíclico por tres días y terapia de soporte. En caso de lograr remisión completa de la leucemia recibieron uno o dos ciclos de consolidación con citarabina. El tratamiento paliativo consistió en medidas de soporte o quimioterapia oral o intravenosa en dosis bajas. Resultados: del grupo de QTI siete pacientes alcanzaron remisión completa, comparados con uno del grupo de quimioterapia paliativa. La supervivencia global fue de 13.25 meses para los pacientes con QTI y de 3.35 meses para los pacientes de quimioterapia paliativa (p < 0.05). Conclusión: es posible que los pacientes con LMA mayores de 60 años de edad se beneficien de recibir QTI, comparada con la quimioterapia paliativa.

  10. Study of the decay of /sup 254/Es and /sup 250/Bk

    SciTech Connect

    Popov, Yu.S.; Kovantsev, V.N.; Elesin, A.A.; Timofeev, G.A.

    1988-09-01

    The emission spectra of /sup 254/Es and /sup 250/Bk have been studied by the methods of semiconductor alpha, gamma, and x-ray spectrometry. The results of the determination of the intensities of the gamma rays of /sup 254/Es at 63, 85, 290, and 385 keV have been presented: 1.6 /plus minus/ 0.4, 0.16 /plus minus/ 0.7, 0.3 /plus minus/ 0.1, and 0.4 /plus minus/ 0.1%, respectively. The ratio of the intensities of the gamma rays of /sup 250/Bk at 988.96 keV and at 1028.58 and 1031.76 keV has been calculated and found to be equal to 1.113 /plus minus/ 0.0037. The intensities of the x-rays of /sup 250/Bk of the L/sub 3/, L/sub /alpha//, L/sub /beta//, and L/sub /gamma// series form a 6:87:100:21 ratio. The half periods have been determined: /sup 254/Es, 266 /plus minus/ 4 days; /sup 250/Bk, 186.2 /plus minus/ 1.1 min.

  11. Forecasting VaR and ES of stock index portfolio: A Vine copula method

    NASA Astrophysics Data System (ADS)

    Zhang, Bangzheng; Wei, Yu; Yu, Jiang; Lai, Xiaodong; Peng, Zhenfeng

    2014-12-01

    Risk measurement has both theoretical and practical significance in risk management. Using daily sample of 10 international stock indices, firstly this paper models the internal structures among different stock markets with C-Vine, D-Vine and R-Vine copula models. Secondly, the Value-at-Risk (VaR) and Expected Shortfall (ES) of the international stock markets portfolio are forecasted using Monte Carlo method based on the estimated dependence of different Vine copulas. Finally, the accuracy of VaR and ES measurements obtained from different statistical models are evaluated by UC, IND, CC and Posterior analysis. The empirical results show that the VaR forecasts at the quantile levels of 0.9, 0.95, 0.975 and 0.99 with three kinds of Vine copula models are sufficiently accurate. Several traditional methods, such as historical simulation, mean-variance and DCC-GARCH models, fail to pass the CC backtesting. The Vine copula methods can accurately forecast the ES of the portfolio on the base of VaR measurement, and D-Vine copula model is superior to other Vine copulas.

  12. Assessment of human thermal perception in the hot-humid climate of Dar es Salaam, Tanzania

    NASA Astrophysics Data System (ADS)

    Ndetto, Emmanuel L.; Matzarakis, Andreas

    2016-06-01

    Dar es Salaam, Tanzania, is a typical African city along the Indian Ocean coast, and therefore an important urban area to examine human thermal perception in the hot-humid tropical climate. Earlier research on human bioclimate at Dar es Salaam indicated that heat stress prevails during the hot season from October to March, peaking between December and February, particularly the early afternoons. In order to assess the human thermal perception and adaptation, two popular places, one at an urban park and another at a beach environment, were selected and questionnaire surveys were conducted in August-September 2013 and January 2014, concurrently with local micro-meteorological measurements at survey locations. The thermal conditions were quantified in terms of the thermal index of the physiologically equivalent temperature (PET) using the micro-scale climate model RayMan. The thermal comfort range of human thermal comfort and the local thermal adaptive capacity were determined in respect to the thermal index by binning thermal sensation votes. The thermal comfort range was found to be well above that in temperate climates at about 23-31 °C of PET. The study could significantly contribute to urban planning in Dar es Salaam and other coastal cities in the tropics.

  13. The HadGEM2-ES implementation of CMIP5 centennial simulations

    SciTech Connect

    Jones, C. D.; Hughes, JK; Bellouin, N; Hardimann, SC; Jones, GS; Knight, J; Liddicoat, S; O'Connor, FM; Andres, Robert Joseph; Bell, C; Boo, K-O; Bozzo, A; Butchart, N; Cadule, P; Corbin, KD; Doutriaux-Boucher, M; Friedlingstein, P; Gornall, J; Gray, L; Halloran, PR; Hurtt, G; Ingram, WJ; Lamarque, J-F; Law, RM; Meinshausen, M; Osprey, S; Palin, E J; Parsons Chini, L; Raddatz, T; Sanderson, M G; Sellar, A A; Schurer, A; Valdes, P; Wood, N; Woodward, S; Yoshioka, M; Zerroukat, M

    2011-01-01

    The scientific understanding of the Earth s climate system, including the central question of how the climate system is likely to respond to human-induced perturbations, is comprehensively captured in GCMs and Earth System Models (ESM). Diagnosing the simulated climate response, and comparing responses across different models, is crucially dependent on transparent assumptions of how the GCM/ESM has been driven especially because the implementation can involve subjective decisions and may differ between modelling groups performing the same experiment. This paper outlines the climate forcings and setup of the Met Office Hadley Centre ESM, HadGEM2-ES for the CMIP5 set of centennial experiments. We document the prescribed greenhouse gas concentrations, aerosol precursors, stratospheric and tropospheric ozone assumptions, as well as implementation of land-use change and natural forcings for the HadGEM2-ES historical and future experiments following the Representative Concentration Pathways. In addition, we provide details of how HadGEM2-ES ensemble members were initialised from the control run and how the palaeoclimate and AMIP experiments, as well as the emissiondriven RCP experiments were performed.

  14. Arsenic speciation in chinese seaweeds using HPLC-ICP-MS and HPLC-ES-MS.

    PubMed

    Van Hulle, Marijn; Zhang, Chao; Zhang, Xinrong; Cornelis, Rita

    2002-05-01

    Three common Chinese edible seaweeds, one brown (Laminaria japonica) and two red (Porphyra crispata and Eucheuma denticulatum), were examined for their total arsenic content. The As species were extracted with yields of 76.4, 69.8 and 25.0%, respectively. Anion-exchange and cation-exchange high-performance liquid chromatography (HPLC) in combination with inductively coupled plasma mass spectrometry (ICP-MS) were used for the separation of the different arsenic species in two of the three seaweed extracts (Laminaria and Porphyra). The main arsenic species in the algal extracts are arseno sugars, although it has been shown that the Laminaria seaweed contains significant amounts of dimethylarsinic acid (DMA). HPLC was coupled with electrospray mass spectrometry (ES-MS) for structural confirmation of the arsenic species. The mass spectrometer settings for the arseno sugars were optimised using standards. The conclusions drawn on the basis of HPLC-ICP-MS were confirmed by the HPLC-ES-MS data. The HPLC-ES-MS method is capable of determining both arseno sugars and DMA in the seaweeds. The unknown compounds seen in the HPLC-ICP-MS chromatogram of Laminaria could not be ascribed to trimethylarsenic oxide or tetramethylarsonium ion. PMID:12081041

  15. Deployment and Operation of the ES-3100 Type B Shipping Container

    SciTech Connect

    Arbital, J. G.; Tousley, D. R.: Miller, D. B.

    2006-07-27

    The U.S. Department of Energy (DOE) National Nuclear Security Administration (NNSA) is shipping, for disposition purposes, bulk quantities of fissile materials, primarily highly enriched uranium (HEU). The U.S. Department of Transportation (DOT) specification 6M container has been the workhorse for NNSA and many other shippers of radioactive material since the 1980s. However, the 6M does not conform to the packaging requirements in the Code of Federal Regulations (10 CFR 71) and, for that reason, is being phased out for use in the DOE secure transportation system by the end of 2006. BWXT Y-12 developed and licensed the ES-3100 container to replace the DOT 6M. The ES-3100 was certified by the Nuclear Regulatory Commission (NRC) in April 2006. The process of deploying the new package began in June 2005 and is planned to be completed in July 2006. The package will be fully operational and completely replace the DOT 6M at the Y-12 National Security Complex (Y-12) by October 2006. This paper reviews the deployment process and the mock loading station that was installed at National Transportation Research Center (NTRC) of Oak Ridge National Laboratory. Specialized equipment, tools, and instrumentation that support the handling and loading operations of the ES-3100 are described in detail. Loading options for other user sites are explored in preparation for deployment of this new state-of-the-art shipping container throughout the DOE complex and the private sector.

  16. Sociocultural factors that reduce risks of homicide in Dar es Salaam: a case control study

    PubMed Central

    Kibusi, Stephen Matthew; Ohnishi, Mayumi; Outwater, Anne; Seino, Kaoruko; Kizuki, Masashi; Takano, Takehito

    2013-01-01

    Objectives This study was performed to examine the potential contributions of sociocultural activities to reduce risks of death by homicide. Methods This study was designed as a case control study. Relatives of 90 adult homicide victims in Dar es Salaam Region, Tanzania, in 2005 were interviewed. As controls, 211 participants matched for sex and 5-year age group were randomly selected from the same region and interviewed regarding the same contents. Results Bivariate analysis revealed significant differences between victims and controls regarding educational status, occupation, family structure, frequent heavy drinking, hard drug use and religious attendance. Conditional logistic regression analysis indicated that the following factors were significantly related to not becoming victims of homicide: being in employment (unskilled labour: OR=0.04, skilled labour: OR=0.07, others: OR=0.04), higher educational status (OR=0.02), residence in Dar es Salaam after becoming an adult (compared with those who have resided in Dar es Salaam since birth: OR=3.95), living with another person (OR=0.07), not drinking alcohol frequently (OR=0.15) and frequent religious service attendance (OR=0.12). Conclusions Frequent religious service attendance, living in the same place for a long time and living with another person were shown to be factors that contribute to preventing death by homicide, regardless of place of residence and neighbourhood environment. Existing non-structural community resources and social cohesive networks strengthen individual and community resilience against violence. PMID:23322260

  17. VizieR Online Data Catalog: TrES-4b RV and Ic curves (Sozzetti+, 2015)

    NASA Astrophysics Data System (ADS)

    Sozzetti, A.; Bonomo, A. S.; Biazzo, K.; Mancini, L.; Damasso, M.; Desidera, S.; Gratton, R.; Lanza, A. F.; Poretti, E.; Rainer, M.; Malavolta, L.; Affer, L.; Barbieri, M.; Bedin, L. R.; Boccato, C.; Bonavita, M.; Borsa, F.; Ciceri, S.; Claudi, R. U.; Gandolfi, D.; Giacobbe, P.; Henning, T.; Knapic, C.; Latham, D. W.; Lodato, G.; Maggio, A.; Maldonado, J.; Marzari, F.; Martinez Fiorenzano, A. F.; Micela, G.; Molinari, E.; Mordasini, C.; Nascimbeni, V.; Pagano, I.; Pedani, M.; Pepe, F.; Piotto, G.; Santos, N.; Scandariato, G.; Shkolnik, E.; Southworth, J.

    2015-06-01

    The TrES-4 system was observed with HARPS-N on 17 individual epochs between March 2013 and July 2014. We carried out Ic-band precision photometric observations of two complete transit events of TrES-4 b with the CAHA 1.23-m on UT 2013 July 6 and UT 2014 June 30. (2 data files).

  18. 75 FR 8326 - AES ES Westover, LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-24

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission AES ES Westover, LLC; Supplemental Notice That Initial Market- Based Rate... notice in the above-referenced proceeding of AES ES Westover, LLC's application for market-based...

  19. Building trust and confidence in laboratory ES and H policy and practices

    SciTech Connect

    Graf, J.

    2000-08-01

    This report describes a successful pilot event among LANL employees that can see as a model for employee involvement and community input. The conference was designed to begin building trust and confidence in Laboratory policy and practices in the area of Environment, Safety, and Health (ES and H). It represents a concrete step toward fostering better relationships among Lab employees and creating a new, innovative approach to communication that can also be used to build trust in the larger community. Based on the proven methods of the National Issues Forums and the Jefferson Center Citizen Jury Process, this conference enabled management to learn more about the thoughts and advice of LANL employees, During the course of the day, a random sample of Lab employees representing the LANL workforce learned about issues of health, safety and the environment, and some of the options available to increase trustworthiness in these areas. These Employee Advisors then discussed the options at some length and presented recommendations to senior Lab managers in the role of Decision Makers. At the end of the day, the participants offered their reflections and discussed what they learned during the conference, and Decision Makers responded to what they heard. The most common view expressed by the Employee Advisors was that a bottom-up approach was necessary to develop more relevant ES and H policies. They were unanimous in their desire for more employee inclusion into the decision making process. All Employee Advisors were in support of a Lab wide survey to determine employee concerns about ES and H issues. After listening to the deliberation, the Decision Makers responded with several commitments. The most significant was the pledge to meet with Employee Advisors by the end of February to discuss the status of their recommendations on ES and H policy and practices. The ensuing follow-up meeting explored employee concerns in greater depth resulting in forward-looking action steps

  20. Spectroscopy and dynamics of 5f states of Es{sup 3+} in LaF{sub 3}

    SciTech Connect

    Beitz, J.V.; Williams, C.W.; Liu, G.K.

    1997-11-01

    Using time- and wavelength-resolved laser-induced fluorescence methods, the 5f state spectroscopy and photodynamics of {sup 253}Es{sup 3+} in LaF{sub 3} have been investigated. Based on an effective operator Hamiltonian model and approximating the metal ion site symmetry as C{sub 2V}, a set of crystal field parameters has been obtained that fit the 56 assigned levels associated with the 7 states of Es{sup 3+} that were observed. The {sup 5}F{sub 5} emitting state of Es{sup 3+} exhibited a decay rate that approached the expected purely radiative decay of the state. This suggests that the shorter lifetime previously found for this state of Es{sup 3+} in LaCl{sub 3} arose from radiation damage induced by alpha decay of {sup 253}Es.

  1. Modelling the horizontal structure of mid-latitude E(s) from its refraction effects on F-region echoes

    NASA Astrophysics Data System (ADS)

    Barnes, R. I.

    1991-02-01

    The observation of the refraction of F-region echoes presently reported implies that significant horizontal structure exists within some E(s) clouds. A modeling of the data collected with the Bribie Island HF radar indicates that wind shear variations can account for examples in which irregularities either pass through, or pass with, existing layers of E(s) which create the cloudy, nonblanketing variety of E(s). The blanketing variety of E(s) seems to have little or no horizontal structure, and most likely drifts with the same velocity as the irregularities which produce nonblanketing E(s). Irregularities with strong horizontal gradients act as diverging lenses, leading to an overestimation of cloud size via simple occulting geometry.

  2. A Spitzer five-band analysis of the Jupiter-sized planet TrES-1

    SciTech Connect

    Cubillos, Patricio; Harrington, Joseph; Foster, Andrew S. D.; Lust, Nate B.; Hardy, Ryan A.; Bowman, M. Oliver; Madhusudhan, Nikku

    2014-12-10

    With an equilibrium temperature of 1200 K, TrES-1 is one of the coolest hot Jupiters observed by Spitzer. It was also the first planet discovered by any transit survey and one of the first exoplanets from which thermal emission was directly observed. We analyzed all Spitzer eclipse and transit data for TrES-1 and obtained its eclipse depths and brightness temperatures in the 3.6 μm (0.083% ± 0.024%, 1270 ± 110 K), 4.5 μm (0.094% ± 0.024%, 1126 ± 90 K), 5.8 μm (0.162% ± 0.042%, 1205 ± 130 K), 8.0 μm (0.213% ± 0.042%, 1190 ± 130 K), and 16 μm (0.33% ± 0.12%, 1270 ± 310 K) bands. The eclipse depths can be explained, within 1σ errors, by a standard atmospheric model with solar abundance composition in chemical equilibrium, with or without a thermal inversion. The combined analysis of the transit, eclipse, and radial-velocity ephemerides gives an eccentricity of e=0.033{sub −0.031}{sup +0.015}, consistent with a circular orbit. Since TrES-1's eclipses have low signal-to-noise ratios, we implemented optimal photometry and differential-evolution Markov Chain Monte Carlo (MCMC) algorithms in our Photometry for Orbits, Eclipses, and Transits pipeline. Benefits include higher photometric precision and ∼10 times faster MCMC convergence, with better exploration of the phase space and no manual parameter tuning.

  3. Numerical simulation of groundwater flow in Dar es Salaam Coastal Plain (Tanzania)

    NASA Astrophysics Data System (ADS)

    Luciani, Giulia; Sappa, Giuseppe; Cella, Antonella

    2016-04-01

    They are presented the results of a groundwater modeling study on the Coastal Aquifer of Dar es Salaam (Tanzania). Dar es Salaam is one of the fastest-growing coastal cities in Sub-Saharan Africa, with with more than 4 million of inhabitants and a population growth rate of about 8 per cent per year. The city faces periodic water shortages, due to the lack of an adequate water supply network. These two factors have determined, in the last ten years, an increasing demand of groundwater exploitation, carried on by quite a number of private wells, which have been drilled to satisfy human demand. A steady-state three dimensional groundwater model has been set up by the MODFLOW code, and calibrated with the UCODE code for inverse modeling. The aim of the model was to carry out a characterization of groundwater flow system in the Dar es Salaam Coastal Plain. The inputs applied to the model included net recharge rate, calculated from time series of precipitation data (1961-2012), estimations of average groundwater extraction, and estimations of groundwater recharge, coming from zones, outside the area under study. Parametrization of the hydraulic conductivities was realized referring to the main geological features of the study area, based on available literature data and information. Boundary conditions were assigned based on hydrogeological boundaries. The conceptual model was defined in subsequent steps, which added some hydrogeological features and excluded other ones. Calibration was performed with UCODE 2014, using 76 measures of hydraulic head, taken in 2012 referred to the same season. Data were weighted on the basis of the expected errors. Sensitivity analysis of data was performed during calibration, and permitted to identify which parameters were possible to be estimated, and which data could support parameters estimation. Calibration was evaluated based on statistical index, maps of error distribution and test of independence of residuals. Further model

  4. Residents’ perceptions of institutional performance in water supply in Dar es Salaam

    NASA Astrophysics Data System (ADS)

    Mwakalila, Shadrack

    This paper addresses the performance of institutions in water supply systems for improving social and economic benefits of people living in Dar es Salaam city. The methods employed in field data and information collection included interviews, questionnaire, focus group discussions and participatory observation. Kinondoni and Ilala Districts were used as case study. The study revealed that, the main water sources in the study areas are boreholes, shallow wells, rain water and water vendors. Other minor sources are piped water and natural water sources, such as rivers and streams. The supply of piped water by Dar es Salaam Water Sewerage and Sanitation Company (DAWASA/DAWASCO) meets only 45% of the total water demands. Individuals own and sell water from boreholes, shallow wells, piped water connected to their individual houses and natural wells located in their individual plots. The price of one 20 l bucket of water from a water vendor depends on the availability of water and the distance walked from the water source to the customer. Majority of the respondents (77.5%) indicated that individual water delivery systems provide sufficient water as compared to five years ago in the study areas. Few of the respondents (6.3%) said individual water delivery systems have no capacity to provide sufficient water while 16.3% indicate that individual water delivery systems provide moderate water supply but are important in supplementing other water providers in the study areas. The study reveals that a majority of the local population are satisfied with the capacity of individual water delivery systems in providing water for household uses. This paper recommends some improvements to be done to water supply systems in the Dar es Salaam city.

  5. T-Cell Recognition of Mycobacterial GroES Peptides in Thai Leprosy Patients and Contacts

    PubMed Central

    Chua-Intra, Boosbun; Peerapakorn, Somchai; Davey, Nick; Jurcevic, Stipo; Busson, Marc; Vordermeier, H. Martin; Pirayavaraporn, Charoon; Ivanyi, Juraj

    1998-01-01

    We report here the mapping of T-cell-stimulatory determinants of the GroES 10-kDa heat shock protein homologues from Mycobacterium leprae and Mycobacterium tuberculosis, which are known as major immunogens in mycobacterial infections. Peripheral blood mononuclear cells (PBMC) from treated tuberculoid leprosy or lepromatous leprosy patients and from healthy household or hospital staff contacts of the patients were cultured with 20 16-mer peptides covering the entire sequences of both M. leprae and M. tuberculosis GroES. The total number of recognized peptides was found to be the largest in family contacts, while responder frequencies to the individual tested peptides varied (5 to 80%) with specificity between the patient and contact groups. Proliferative responses to some peptides showed positive or negative associations of low statistical significance with DR and DQ alleles, though responses to most GroES peptides were genetically permissive. Notably, the sequence of the 25–40 peptide of M. leprae, but not that of M. tuberculosis, was more frequently stimulatory in tuberculoid leprosy patients than in either group of sensitized healthy contacts. This peptide bound to a number of HLA-DR molecules, of which HLA-DRB5*0101 had the strongest affinity. The epitope core binding to this allele was localized to the 29-to-37 sequence, and its key residue was localized to the M. leprae-specific glutamic acid at position 32. This epitope may be of interest for the development of a blood test- or skin test-based diagnostic reagent for tuberculoid leprosy, subject to further clinical evaluation in untreated patients. PMID:9746595

  6. Study of optical microvariability in the blazar 1ES1011+496

    NASA Astrophysics Data System (ADS)

    Sosa, M. S.; von Essen, C.; Cellone, S. A.; Andruchow, I.; Schmitt, J. H. M. M.

    We carried out a study of photometric variability of the blazar 1ES1011+496 using the 1.20 m Oskar Lühning telescope located at Ham- burger Sternwarte Institute, Germany. This object has been detected at hight energies ( 200 GeV), so it is of interest to characterize its behavior in the optical range. We obtained the light curves in B, V and R bands through dif- ferential photometry, with a time resolution of 15 minutes over 8 nights. We did not detect inter-night variability, but we detected a marginally sig- nificant variability in temporal scales of a few days.

  7. Multiwavelength Observations of the Very High Energy Blazar 1ES 2344+514

    NASA Astrophysics Data System (ADS)

    Acciari, V. A.; Aliu, E.; Arlen, T.; Aune, T.; Beilicke, M.; Benbow, W.; Boltuch, D.; Bugaev, V.; Cannon, A.; Ciupik, L.; Cogan, P.; Colin, P.; Dickherber, R.; Falcone, A.; Fegan, S. J.; Finley, J. P.; Fortin, P.; Fortson, L. F.; Furniss, A.; Gall, D.; Gillanders, G. H.; Grube, J.; Guenette, R.; Gyuk, G.; Hanna, D.; Holder, J.; Horan, D.; Hui, C. M.; Humensky, T. B.; Imran, A.; Kaaret, P.; Karlsson, N.; Kertzman, M.; Kieda, D.; Kildea, J.; Konopelko, A.; Krawczynski, H.; Krennrich, F.; Lang, M. J.; LeBohec, S.; Maier, G.; Moriarty, P.; Mukherjee, R.; Ong, R. A.; Otte, A. N.; Pandel, D.; Perkins, J. S.; Pichel, A.; Pohl, M.; Quinn, J.; Ragan, K.; Reynolds, P. T.; Rose, H. J.; Schroedter, M.; Sembroski, G. H.; Smith, A. W.; Steele, D.; Swordy, S. P.; Theiling, M.; Toner, J. A.; Varlotta, A.; Vassiliev, V. V.; Vincent, S.; Wagner, R.; Wakely, S. P.; Ward, J. E.; Weekes, T. C.; Weinstein, A.; Weisgarber, T.; Williams, D. A.; Wissel, S.; Wood, M.; Zitzer, B.

    2011-09-01

    Multiwavelength observations of the high-frequency-peaked blazar 1ES 2344+514 were performed from 2007 October to 2008 January. The campaign represents the first contemporaneous data on the object at very high energy (VHE, E >100 GeV) γ-ray, X-ray, and UV energies. Observations with VERITAS in VHE γ-rays yield a strong detection of 20σ with 633 excess events in a total exposure of 18.1 hr live time. A strong VHE γ-ray flare on 2007 December 7 is measured at F(>300 GeV) = (6.76 ± 0.62) × 10-11 photons cm-2 s-1, corresponding to 48% of the Crab Nebula flux. Excluding this flaring episode, nightly variability at lower fluxes is observed with a time-averaged mean of F(>300 GeV) = (1.06 ± 0.09) × 10-11 photons cm-2 s-1 (7.6% of the Crab Nebula flux). The differential photon spectrum between 390 GeV and 8.3 TeV for the time-averaged observations excluding 2007 December 7 is well described by a power law with a photon index of Γ = 2.78 ± 0.09stat ± 0.15syst. On the flaring night of 2007 December 7 the measured VHE γ-ray photon index was Γ = 2.43 ± 0.22stat ± 0.15syst. Over the full period of VERITAS observations contemporaneous X-ray and UV data were taken with Swift and RXTE. The measured 2-10 keV flux ranged by a factor of ~7 during the campaign. On 2007 December 8 the highest ever observed X-ray flux from 1ES 2344+514 was measured by Swift X-ray Telescope at a flux of F(2-10 keV) = (6.28 ± 0.31) × 10-11 erg cm-2 s-1. Evidence for a correlation between the X-ray flux and VHE γ-ray flux on nightly timescales is indicated with a Pearson correlation coefficient of r = 0.60 ± 0.11. Contemporaneous spectral energy distributions (SEDs) of 1ES 2344+514 are presented for two distinct flux states. A one-zone synchrotron self-Compton (SSC) model describes both SEDs using parameters consistent with previous SSC modeling of 1ES 2344+514 from non-contemporaneous observations.

  8. Multiple Mechanisms of Uranium Immobilization by Cellulomonas sp. Strain ES6

    SciTech Connect

    Sivaswamy, Vaideeswaran; Boyanov, Maxim I.; Peyton, Brent M.; Viamajala, Sridhar; Gerlach, Robin; Apel, William; Sani, Rajesh K.; Dohnalkova, Alice; Kemner, Kenneth M.; Borch, Thomas

    2011-02-24

    Removal of hexavalent uranium (U(VI)) from aqueous solution was studied using a Gram-positive facultative anaerobe, Cellulomonas sp. strain ES6, under anaerobic, non-growth conditions in bicarbonate and PIPES buffers. Inorganic phosphate was released by cells during the experiments providing ligands for formation of insoluble U(VI) phosphates. Phosphate release was most probably the result of anaerobic hydrolysis of intracellular polyphosphates accumulated by ES6 during aerobic growth. Microbial reduction of U(VI) to U(IV) was also observed. However, the relative magnitudes of U(VI) removal by abiotic (phosphate-based) precipitation and microbial reduction depended on the buffer chemistry. In bicarbonate buffer, X-ray absorption fine structure (XAFS) spectroscopy showed that U in the solid phase was present primarily as a non-uraninite U(IV) phase, whereas in PIPES buffer, U precipitates consisted primarily of U(VI)-phosphate. In both bicarbonate and PIPES buffer, net release of cellular phosphate was measured to be lower than that observed in U-free controls suggesting simultaneous precipitation of U and PO3-4 . In PIPES, U(VI) phosphates formed a significant portion of U precipitates and mass balance estimates of U and P along with XAFS data corroborate this hypothesis. High-resolution transmission electron microscopy (HRTEM) and energy dispersive X-ray spectroscopy (EDS) of samples from PIPES treatments indeed showed both extracellular and intracellular accumulation of U solids with nanometer sized lath structures that contained U and P. In bicarbonate, however, more phosphate was removed than required to stoichiometrically balance the U(VI)/U(IV) fraction determined by XAFS, suggesting that U(IV) precipitated together with phosphate in this system. When anthraquinone-2,6-disulfonate (AQDS), a known electron shuttle, was added to the experimental reactors, the dominant removal mechanism in both buffers was reduction to a non-uraninite U(IV) phase. Uranium

  9. Multiple mechanisms of uranium immobilization by Cellulomonas sp. strain ES6.

    PubMed

    Sivaswamy, Vaideeswaran; Boyanov, Maxim I; Peyton, Brent M; Viamajala, Sridhar; Gerlach, Robin; Apel, William A; Sani, Rajesh K; Dohnalkova, Alice; Kemner, Kenneth M; Borch, Thomas

    2011-02-01

    Removal of hexavalent uranium (U(VI)) from aqueous solution was studied using a Gram-positive facultative anaerobe, Cellulomonas sp. strain ES6, under anaerobic, non-growth conditions in bicarbonate and PIPES buffers. Inorganic phosphate was released by cells during the experiments providing ligands for formation of insoluble U(VI) phosphates. Phosphate release was most probably the result of anaerobic hydrolysis of intracellular polyphosphates accumulated by ES6 during aerobic growth. Microbial reduction of U(VI) to U(IV) was also observed. However, the relative magnitudes of U(VI) removal by abiotic (phosphate-based) precipitation and microbial reduction depended on the buffer chemistry. In bicarbonate buffer, X-ray absorption fine structure (XAFS) spectroscopy showed that U in the solid phase was present primarily as a non-uraninite U(IV) phase, whereas in PIPES buffer, U precipitates consisted primarily of U(VI)-phosphate. In both bicarbonate and PIPES buffer, net release of cellular phosphate was measured to be lower than that observed in U-free controls suggesting simultaneous precipitation of U and PO₄³⁻. In PIPES, U(VI) phosphates formed a significant portion of U precipitates and mass balance estimates of U and P along with XAFS data corroborate this hypothesis. High-resolution transmission electron microscopy (HR-TEM) and energy dispersive X-ray spectroscopy (EDS) of samples from PIPES treatments indeed showed both extracellular and intracellular accumulation of U solids with nanometer sized lath structures that contained U and P. In bicarbonate, however, more phosphate was removed than required to stoichiometrically balance the U(VI)/U(IV) fraction determined by XAFS, suggesting that U(IV) precipitated together with phosphate in this system. When anthraquinone-2,6-disulfonate (AQDS), a known electron shuttle, was added to the experimental reactors, the dominant removal mechanism in both buffers was reduction to a non-uraninite U(IV) phase. Uranium

  10. Department of Energy Nanoscale Science Research Centers: Approach to Nanomaterial ES&H

    SciTech Connect

    None, None

    2008-05-12

    The following non-mandatory guidance is intended for the Nanoscale Science Research Centers (NSRCs) funded by the Basic Energy Sciences program office under the U.S. Department of Energy's Office of Science. It describes practices thought appropriate to the management of environmental, safety and health (ES&H) concerns associated with laboratory-scale operations involving the design, synthesis, or characterization of engineered nanomaterials, In general, it is intended to apply to precursors, intermediates, and wastes used during, or resulting from synthesizing such nanomaterials. In general, it is not intended to apply to materials for which an occupational exposure limit has been established.

  11. The Long-term Light Variation of BL Lac Object 1ES 1959+650

    NASA Astrophysics Data System (ADS)

    Yuan, Yu-hai; Liu, Fu-qing

    2014-07-01

    According to the data of optical observations of the Tuorla Observatory in Finland, using the power spectrum method, DCF (Discrete Correlation Function) method, and Jurkevich method, respectively, we analyzed the periodicity of the long-term light variation of the BL Lac object 1ES 1959+650, and obtained its light period to be P =1.4±0.3 yr. Assuming that the origin of the periodicity is concerned with the accretion disk, we obtained the region where the instability of this source occurs being R =9.65 Rg, here Rg represents the Schwarzschild radius.

  12. Les tumeurs conjonctives cutanées: à propos de 121 cas

    PubMed Central

    Hazmiri, Fatima Ezzahra; Fakhri, Anas; Rais, Hanane; Akhdari, Nadia; Amal, Said; Belaabidia, Badia

    2014-01-01

    Les tumeurs conjonctives cutanées sont des tumeurs dermiques et/ou hypodermiques relativement fréquentes. Elles sont dominées par les tumeurs bénignes. A travers une série de 121 cas, nous avons étudié le profil épidémiologique, anatomopathologique et évolutif de ces tumeurs. C'est une étude rétrospective réalisée au service d'anatomie pathologique du CHU Mohammed VI de Marrakech entre 2004 et 2012. Il s'agit de 121 patients. La moyenne d’âge était de 36 ans (1-80ans). Le sex-ratio H/F était de 1,12. La tumeur avait un aspect nodulaire dans 90% des cas. Le membre inférieur était la localisation la plus fréquente (30,5%). L’étude anatomopathologique a porté sur un matériel biopsique dans 100% des cas. Soixante-neuf pour cent de ces tumeurs étaient bénignes. Elles étaient représentées essentiellement par les tumeurs vasculaires, suivies par les tumeurs fibreuses et fibro-histiocytaires. Trente et un pour cent des tumeurs étaient malignes. Il s'agissait essentiellement de tumeurs fibreuses et fibro-histiocytaires, suivies de tumeurs vasculaires. L’étude immunohistochimique était réalisée dans 2cas. Le traitement chirurgical était entrepris dans 73% des cas. L’évolution était précisée dans 19% des cas avec une évolution favorable dans 13% des cas. Un cas de décès et 2 cas de récidive étaient notés. Les tumeurs conjonctives cutanées bénignes sont de bon pronostic, mais posent un problème majeur de nosologie et de classification. D'autre part, la prise en charge diagnostique et thérapeutique ainsi que l’évaluation pronostique des sarcomes cutanés restent difficiles. PMID:25379113

  13. CHANDRA Detection of the AM CVn Binary ES Cet (KUV 01584-0939)

    NASA Technical Reports Server (NTRS)

    Strohmayer, Tod E.

    2004-01-01

    We report on Chandra ACE observations of the ultracompact AM CVn binary ES Cet. This object has a 10.3 minute binary period and is the most compact of the confirmed AM CVn systems. We have, for the first time, unambiguously detected the X-ray counterpart to ES Cet. In a 20 ksec ACIS-S image a point-like X-ray source is found within 1 sec. of the catalogued optical position. The mean countrate in ACIS-S is 0.013/s, and there is no strong evidence for variability. We folded the X-ray data using the optical ephemeris of Warner & Woudt, but did not detect any significant modulation. If an approx. = 100% modulation similar to those seen in the ultracompact candidates V407 Vu1 and Rx J0806.3+1527 were present then we would have detected it. The upper limit (3(sigma)) to any modulation at the putative orbital period is approx. 40% (rms). We extract the first X-ray spectrum from ES Cet, and find that it is not well described by simple continuum models. We find suggestive evidence for discrete spectral components at approx. 470 and 890 eV, that can be modelled as gaussian emission lines. In comparison with recent X-ray detections of nitrogen and neon in another AM CVn system (GP Com), it appears possible that these features may represent emission lines from these same elements; however, deeper spectroscopy will be required to confirm this. Our best spectral model includes a black body continuum with kT = 0.8 keV along with the gaussian lines. The 0.2 - 5 keV X-ray flux was approx. 7 x 10(exp -14) ergs/sq cm s. The luminosity implied by this flux for any reasonable distance is much smaller than that expected for a mass accretion rate as high as m = 10(exp -8) solar mass/yr, suggesting that the bulk of the accretion luminosity is below 100 eV and not seen with Chandra. We discuss the implications of our results for the nature of ES Cet.

  14. Les étoiles invitées au secours des astrophysiciens

    NASA Astrophysics Data System (ADS)

    Bonnet-Bidaud, J. M.

    2007-04-01

    Le message des étoiles invitées. Comment connaitre précisément la date d'explosion d'une supernova dont on observe les vestiges ? Notamment en consultant les registres astronomiques tenus par les Anciens ! Il y a 2000 ans déjà, les astronomes chinois notaient rigoureusement chaque apparition d'une "étoile invitée" dans le ciel. Une mine d'or pour les chercheurs d'aujourd'hui.

  15. A Double WAP Domain-Containing Protein Es-DWD1 from Eriocheir sinensis Exhibits Antimicrobial and Proteinase Inhibitory Activities

    PubMed Central

    Guo, Xiao-Nv; Yu, Ai-Qing; Wu, Min-Hao; Tan, Shang-Jian; Zhu, You-Ting; Li, Wei-Wei; Wang, Qun

    2013-01-01

    Whey acidic proteins (WAP) belong to a large gene family of antibacterial peptides, which are critical in the host immune response against microbial invasion. The common feature of these proteins is a single WAP domain maintained by at least one four-disulfide core (4-DSC) structure rich in cysteine residues. In this study, a double WAP domain (DWD)-containing protein, Es-DWD1, was first cloned from the Chinese mitten crab (Eriocheirsinensis). The full-length Es-DWD1cDNA was 1193 bp, including a 411 bp open reading frame (ORF) encoding 136 amino acids with a signal peptide of 22 amino acids in the N-terminus. A comparison with other reported invertebrate and vertebrate sequences revealed the presence of WAP domains characteristic of WAP superfamilies. As determined by quantitative real-time RT-PCR, Es-DWD1 transcripts were ubiquitously expressed in all tissues, but it was up-regulated in hemocytes post-challenge with pathogen-associated molecular patterns (PAMPs). The mature recombinant Es-DWD1 (rEs-DWD1) protein exhibited different binding activities to bacteria and fungus. Moreover, rEs-DWD1 could exert agglutination activities against Bacillus subtilis and Pichiapastoris and demonstrated inhibitory activities against the growth of Staphylococcus aureus, Aeromonas hydrophila and P. pastoris. Furthermore, rEs-DWD1 showed a specific protease inhibitory activity in B. subtilis. Coating of rEs-DWD1 onto agarose beads enhanced encapsulation of the beads by crab hemocytes. Collectively, the results suggest that Es-DWD1 is a double WAP domain containing protein with antimicrobial and proteinase inhibitory activities, which play significant roles in the immunity of crustaceans. PMID:23967346

  16. [A case report of Ewing's sarcoma with a peripheral primitive neuroectodermal tumor (ES/pPNET) in the abdominal cavity].

    PubMed

    Takahashi, Hiroki; Chida, Nobuyuki; Kimura, Kenji; Yamao, Yoko; Shiotuka, Kaori; Ritsuno, Hideaki; Awabuchi, Satoshi; Akoshima, Hiromichi; Sugimura, Mikako; Noguchi, Kenji; Tanabe, Nobukazu; Iwabuchi, Masahiro; Mano, Yutaka; Tadokoro, Keiichi; Suzuki, Hiroyoshi

    2009-10-01

    In this report, we present a rare case of Ewing's sarcoma with a peripheral primitive neuroectodermal tumor (ES/pPNET) arising from the abdominal cavity in a 20-year-old woman. The patient complained of upper abdominal pain. Radiological imaging showed a 15-cm mass penetrating to the proxymal jejunum in the upper abdominal cavity and peritoneal disseminations. Immunohistochemical studies revealed that the tumor was ES/pPNET. Although the patient underwent radiation therapy, she died of the disease two months after diagnosis. ES/pPNET in the abdominal cavity is extremely rare and our case showed aggressive behavior and an unfortunate outcome.

  17. The 69 kDa Escherichia coli maltodextrin glucosidase does not get encapsulated underneath GroES and folds through trans mechanism during GroEL/GroES-assisted folding.

    PubMed

    Paul, Subhankar; Singh, Chanpreet; Mishra, Saroj; Chaudhuri, Tapan K

    2007-09-01

    Escherichia coli chaperonin GroEL and GroES assist in folding of a wide variety of substrate proteins in the molecular mass range of approximately 50 kDa, using cis mechanism, but limited information is available on how they assist in folding of larger proteins. Considering that the central cavity of GroEL can accommodate a non-native protein of approximately 60 kDa, it is important to study the GroEL-GroES-assisted folding of substrate proteins that are large enough for cis encapsulation. In this study, we have reported the mechanism of GroEL/GroES-assisted in vivo and in vitro folding of a 69 kDa monomeric E. coli protein maltodextrin glucosidase (MalZ). Coexpression of GroEL and GroES in E. coli causes a 2-fold enhancement of exogenous MalZ activity in vivo. In vitro, GroEL and GroES in the presence of ATP give rise to a 7-fold enhancement in MalZ refolding. Neither GroEL nor single ring GroEL (SR1) in the presence or absence of ATP could enhance the in vitro folding of MalZ. GroES could not encapsulate GroEL-bound MalZ. All these experimental findings suggested that GroEL/GroES-assisted folding of MalZ followed trans mechanism, whereas denatured MalZ and GroES bound to the opposite rings of a GroEL molecule.

  18. Planetary transit observations at the University Observatory Jena: XO-1b and TrES-1

    NASA Astrophysics Data System (ADS)

    Raetz, St.; Mugrauer, M.; Schmidt, T. O. B.; Roell, T.; Eisenbeiss, T.; Hohle, M. M.; Tetzlaff, N.; Vaňko, M.; Seifahrt, A.; Broeg, Ch.; Koppenhoefer, J.; Neuhäuser, R.

    2009-05-01

    We report on observations of transit events of the transiting planets XO-1b and TrES-1 with a 25 cm telescope of the University Observatory Jena. With the transit timings for XO-1b from all 50 available XO, SuperWASP, Transit Light Curve (TLC)-Project- and Exoplanet Transit Database (ETD)-data, including our own I-band photometry obtained in March 2007, we find that the orbital period is P=(3.941501± 0.000001) d, a slight change by ˜3 s compared to the previously published period. We present new ephemeris for this transiting planet. Furthermore, we present new R-band photometry of two transits of TrES-1. With the help of all available transit times from literature this allows us to refine the estimate of the orbital period: P=(3.0300722± 0.0000002) d. Our observations will be useful for future investigations of timing variations caused by additional perturbing planets and/or stellar spots and/or moons. Based on observations obtained with telescopes of the University Observatory Jena, which is operated by the Astrophysical Institute of the Friedrich-Schiller-University Jena.

  19. Genome-wide nucleosome specificity and function of chromatin remodellers in ES cells

    PubMed Central

    de Dieuleveult, Maud; Yen, Kuangyu; Hmitou, Isabelle; Depaux, Arnaud; Boussouar, Fayçal; Dargham, Daria Bou; Jounier, Sylvie; Humbertclaude, Hélène; Ribierre, Florence; Baulard, Céline; Farrell, Nina P.; Park, Bongsoo; Keime, Céline; Carrière, Lucie; Berlivet, Soizick; Gut, Marta; Gut, Ivo; Werner, Michel; Deleuze, Jean-François; Olaso, Robert; Aude, Jean-Christophe; Chantalat, Sophie; Pugh, B. Franklin; Gérard, Matthieu

    2015-01-01

    Summary ATP-dependent chromatin remodellers allow access to DNA for transcription factors and the general transcription machinery, but whether mammalian chromatin remodellers1–3 target specific nucleosomes to regulate transcription is unclear. Here, we present genome-wide remodeller-nucleosome interaction profiles for Chd1, Chd2, Chd4, Chd6, Chd8, Chd9, Brg1 and Ep400 in mouse embryonic stem (ES) cells. These remodellers bind one or both full nucleosomes that flank MNase-defined nucleosome-free promoter regions (NFRs), where they separate divergent transcription. Surprisingly, large CpG-rich NFRs that extend downstream of annotated transcriptional start sites (TSSs) are nevertheless chromatinized with non-nucleosomal or subnucleosomal histone variants (H3.3 and H2A.Z) and modifications (H3K4me3 and H3K27ac). RNA polymerase (pol) II therefore navigates hundreds of bp of altered chromatin in the sense direction before encountering an MNase-resistant nucleosome at the 3′ end of the NFR. Transcriptome analysis upon remodeller depletion reveals reciprocal mechanisms of transcriptional regulation by remodellers. Whereas at active genes individual remodellers play either positive or negative roles via altering nucleosome stability, at polycomb-enriched bivalent genes the same remodellers act in an opposite manner. These findings indicate that remodellers target specific nucleosomes at the edge of NFRs, where they regulate ES cell transcriptional programs. PMID:26814966

  20. ANALYSIS OF KEPLER'S SHORT-CADENCE PHOTOMETRY FOR TrES-2b

    SciTech Connect

    Kipping, David; Bakos, Gaspar

    2011-05-20

    We present an analysis of 18 short-cadence (SC) transit light curves of TrES-2b using quarter 0 (Q0) and quarter 1 (Q1) from the Kepler Mission. The photometry is of unprecedented precision, 237 ppm minute{sup -1}, allowing for the most accurate determination of the transit parameters yet obtained for this system. Global fits of the transit photometry, radial velocities, and known transit times are used to obtain a self-consistent set of refined parameters for this system, including updated stellar and planetary parameters. Special attention is paid to fitting for limb darkening and eccentricity. We place an upper limit on the occultation depth to be <72.9 ppm to 3{sigma} confidence, indicating TrES-2b has the lowest determined geometric albedo for an exoplanet, of A{sub g} < 0.146. We also produce a transit timing analysis using Kepler's SC data and demonstrate exceptional timing precision at the level of a few seconds for each transit event. With 18 fully sampled transits at such high precision, we are able to produce stringent constraints on the presence of perturbing planets, Trojans, and extrasolar moons. We introduce the novel use of control data to identify phasing effects. We also exclude the previously proposed hypotheses of short-period transit time variation and additional transits but find that the hypothesis of long-term inclination change is neither supported nor refuted by our analysis.

  1. Genome-wide nucleosome specificity and function of chromatin remodellers in ES cells.

    PubMed

    de Dieuleveult, Maud; Yen, Kuangyu; Hmitou, Isabelle; Depaux, Arnaud; Boussouar, Fayçal; Bou Dargham, Daria; Jounier, Sylvie; Humbertclaude, Hélène; Ribierre, Florence; Baulard, Céline; Farrell, Nina P; Park, Bongsoo; Keime, Céline; Carrière, Lucie; Berlivet, Soizick; Gut, Marta; Gut, Ivo; Werner, Michel; Deleuze, Jean-François; Olaso, Robert; Aude, Jean-Christophe; Chantalat, Sophie; Pugh, B Franklin; Gérard, Matthieu

    2016-02-01

    ATP-dependent chromatin remodellers allow access to DNA for transcription factors and the general transcription machinery, but whether mammalian chromatin remodellers target specific nucleosomes to regulate transcription is unclear. Here we present genome-wide remodeller-nucleosome interaction profiles for the chromatin remodellers Chd1, Chd2, Chd4, Chd6, Chd8, Chd9, Brg1 and Ep400 in mouse embryonic stem (ES) cells. These remodellers bind one or both full nucleosomes that flank micrococcal nuclease (MNase)-defined nucleosome-free promoter regions (NFRs), where they separate divergent transcription. Surprisingly, large CpG-rich NFRs that extend downstream of annotated transcriptional start sites are nevertheless bound by non-nucleosomal or subnucleosomal histone variants (H3.3 and H2A.Z) and marked by H3K4me3 and H3K27ac modifications. RNA polymerase II therefore navigates hundreds of base pairs of altered chromatin in the sense direction before encountering an MNase-resistant nucleosome at the 3' end of the NFR. Transcriptome analysis after remodeller depletion reveals reciprocal mechanisms of transcriptional regulation by remodellers. Whereas at active genes individual remodellers have either positive or negative roles via altering nucleosome stability, at polycomb-enriched bivalent genes the same remodellers act in an opposite manner. These findings indicate that remodellers target specific nucleosomes at the edge of NFRs, where they regulate ES cell transcriptional programs.

  2. Discovery of very high energy gamma rays from 1ES 1440+122

    NASA Astrophysics Data System (ADS)

    Archambault, S.; Archer, A.; Barnacka, A.; Behera, B.; Beilicke, M.; Benbow, W.; Berger, K.; Bird, R.; Böttcher, M.; Buckley, J. H.; Bugaev, V.; Cardenzana, J. V.; Cerruti, M.; Chen, X.; Christiansen, J. L.; Ciupik, L.; Collins-Hughes, E.; Connolly, M. P.; Cui, W.; Dickinson, H. J.; Dumm, J.; Eisch, J. D.; Errando, M.; Falcone, A.; Federici, S.; Feng, Q.; Finley, J. P.; Fleischhack, H.; Fortson, L.; Furniss, A.; Gillanders, G. H.; Godambe, S.; Griffin, S.; Griffiths, S. T.; Grube, J.; Gyuk, G.; Håkansson, N.; Hanna, D.; Holder, J.; Hughes, G.; Johnson, C. A.; Kaaret, P.; Kar, P.; Kertzman, M.; Khassen, Y.; Kieda, D.; Krawczynski, H.; Kumar, S.; Lang, M. J.; Madhavan, A. S.; Maier, G.; McArthur, S.; McCann, A.; Meagher, K.; Millis, J.; Moriarty, P.; Nelson, T.; Nieto, D.; de Bhróithe, A. O'Faoláin; Ong, R. A.; Otte, A. N.; Park, N.; Perkins, J. S.; Pohl, M.; Popkow, A.; Prokoph, H.; Pueschel, E.; Quinn, J.; Ragan, K.; Rajotte, J.; Reyes, L. C.; Reynolds, P. T.; Richards, G. T.; Roache, E.; Sembroski, G. H.; Shahinyan, K.; Smith, A. W.; Staszak, D.; Sweeney, K.; Telezhinsky, I.; Tucci, J. V.; Tyler, J.; Varlotta, A.; Vassiliev, V. V.; Wakely, S. P.; Welsing, R.; Wilhelm, A.; Williams, D. A.; Zitzer, B.

    2016-09-01

    The BL Lacertae object 1ES 1440+122 was observed in the energy range from 85 GeV to 30 TeV by the VERITAS array of imaging atmospheric Cherenkov telescopes. The observations, taken between 2008 May and 2010 June and totalling 53 h, resulted in the discovery of γ-ray emission from the blazar, which has a redshift z = 0.163. 1ES 1440+122 is detected at a statistical significance of 5.5 standard deviations above the background with an integral flux of (2.8 ± 0.7stat ± 0.8sys) × 10-12 cm-2 s-1 (1.2 per cent of the Crab Nebula's flux) above 200 GeV. The measured spectrum is described well by a power law from 0.2 to 1.3 TeV with a photon index of 3.1 ± 0.4stat ± 0.2sys. Quasi-simultaneous multiwavelength data from the Fermi Large Area Telescope (0.3-300 GeV) and the Swift X-ray Telescope (0.2-10 keV) are additionally used to model the properties of the emission region. A synchrotron self-Compton model produces a good representation of the multiwavelength data. Adding an external-Compton or a hadronic component also adequately describes the data.

  3. VERITAS and multiwavelength observations of the BL Lacertae object 1ES 1741+196

    NASA Astrophysics Data System (ADS)

    Abeysekara, A. U.; Archambault, S.; Archer, A.; Benbow, W.; Bird, R.; Biteau, J.; Buchovecky, M.; Buckley, J. H.; Bugaev, V.; Byrum, K.; Cardenzana, J. V.; Cerruti, M.; Chen, X.; Christiansen, J. L.; Ciupik, L.; Connolly, M. P.; Cui, W.; Dickinson, H. J.; Dumm, J.; Eisch, J. D.; Errando, M.; Falcone, A.; Feng, Q.; Finley, J. P.; Fleischhack, H.; Flinders, A.; Fortin, P.; Fortson, L.; Furniss, A.; Gillanders, G. H.; Griffin, S.; Grube, J.; Gyuk, G.; Huetten, M.; Hanna, D.; Holder, J.; Humensky, T. B.; Johnson, C. A.; Kaaret, P.; Kar, P.; Kelley-Hoskins, N.; Kertzman, M.; Kieda, D.; Krause, M.; Krennrich, F.; Lang, M. J.; Maier, G.; McArthur, S.; McCann, A.; Meagher, K.; Moriarty, P.; Mukherjee, R.; Nieto, D.; O'Brien, S.; O'Faoláin de Bhróithe, A.; Ong, R. A.; Otte, A. N.; Park, N.; Pelassa, V.; Petrashyk, A.; Petry, D.; Pohl, M.; Popkow, A.; Pueschel, E.; Quinn, J.; Ragan, K.; Ratliff, G.; Reyes, L. C.; Reynolds, P. T.; Reynolds, K.; Richards, G. T.; Roache, E.; Rulten, C.; Santander, M.; Sembroski, G. H.; Shahinyan, K.; Smith, A. W.; Staszak, D.; Telezhinsky, I.; Tucci, J. V.; Tyler, J.; Vincent, S.; Wakely, S. P.; Weiner, O. M.; Weinstein, A.; Wilhelm, A.; Williams, D. A.; Zitzer, B.

    2016-07-01

    We present results from multiwavelength observations of the BL Lacertae object 1ES 1741 + 196, including results in the very high energy γ-ray regime using the Very Energetic Radiation Imaging Telescope Array System (VERITAS). The VERITAS time-averaged spectrum, measured above 180 GeV, is well modelled by a power law with a spectral index of 2.7 ± 0.7stat ± 0.2syst. The integral flux above 180 GeV is (3.9 ± 0.8stat ± 1.0syst) × 10-8 m-2 s-1, corresponding to 1.6 per cent of the Crab nebula flux on average. The multiwavelength spectral energy distribution of the source suggests that 1ES 1741+196 is an extreme-high-frequency-peaked BL Lacertae object. The observations analysed in this paper extend over a period of six years, during which time no strong flares were observed in any band. This analysis is therefore one of the few characterizations of a blazar in a non-flaring state.

  4. The use of social media among adolescents in Dar es Salaam and Mtwara, Tanzania.

    PubMed

    Pfeiffer, Constanze; Kleeb, Matthis; Mbelwa, Alice; Ahorlu, Collins

    2014-05-01

    Social media form part of the rapid worldwide digital development that is re-shaping the life of many young people. While the use of social media by youths is increasingly researched in the North, studies about youth in the South are missing. It therefore remains unclear how social media can be included in interventions that aim at informing young people in many countries of the global South about sexual and reproductive health. This paper presents findings of a mixed-methods study of young people's user behaviour on the internet and specifically of social media as a platform for sexual health promotion in Tanzania. The study used questionnaires with 60 adolescents and in-depth interviews with eight students aged 15 to 19 years in Dar es Salaam, and in Mtwara, Southern Tanzania. Findings show that youth in Dar es Salaam and Mtwara access the internet mainly through mobile phones. Facebook is by far the most popular internet site. Adolescents highlighted their interest in reproductive and sexual health messages and updates being delivered through humorous posts, links and clips, as well as by youth role models like music stars and actors that are entertaining and reflect up-to-date trends of modern youth culture.

  5. Detection and characterization of side population in Ewing's sarcoma SK-ES-1 cells in vitro

    SciTech Connect

    Yang, Min; Zhang, Rui; Yan, Ming; Ye, Zhengxu; Liang, Wei; Luo, Zhuojing

    2010-01-01

    Dye exclusion is a valuable technique to isolate cancer stem cells (CSCs) based on an ability of stem cell to efflux fluorescent DNA-binding dye, especially for tumors without unique surface markers. It has been proven that side population (SP) cells that exclude Hoechst 33342 dye are enriched with stem-like cells in several cancer cell lines. In this study, we isolated and characterized SP cells from human Ewing's sarcoma cell line SK-ES-1 in vitro. SP cells were detected in SK-ES-1 and comprised 1.2% of total cell population. Only SP cells had the capacity to regenerate both SP and non-SP cells. The proliferation rates were similar between SP and non-SP cells. However, the clonogenicity and invasiveness of SP cells were significantly higher than that of non-SP cells. Further characterization of this SP phenotype presented other properties. SP cells exhibited increased multi-drug resistance and the ATP binding cassette protein (ABC) transporters were up-regulated in SP population. These findings suggest that SP cells derived from Ewing's sarcoma play the critical role in tumor metastasis and recurrence and might be an ideal target for clinical therapy.

  6. Expresiones de afecto de madres bilingües, Bilingual mothers' expressions of affect

    PubMed Central

    Shiro, Martha

    2015-01-01

    En la interacción de 10 madres bilingües con sus hijos de 30 meses se analiza la expresión de afectividad en L1 y en L2. Se identificaron las expresiones de emoción, volición y actitud epistémica en 30 interacciones espontáneas: i. 10 madres (español L1) hablando en L1; ii. 10 madres (español L1) hablando en inglés L2; y iii. 10 madres (inglés L1) hablando en L1. Los resultados sugieren que los usos de L2 difieren de los de L1 (inglés o español): la expresión de volición y actitud epistémica se asemeja al inglés L1, mientras que la expresión de las emociones se acerca al español L1. Estos hallazgos permiten explicar los usos del lenguaje expresivo de los bilingües y ayudan a determinar sus efectos en el desarrollo del lenguaje PMID:25844004

  7. The use of social media among adolescents in Dar es Salaam and Mtwara, Tanzania.

    PubMed

    Pfeiffer, Constanze; Kleeb, Matthis; Mbelwa, Alice; Ahorlu, Collins

    2014-05-01

    Social media form part of the rapid worldwide digital development that is re-shaping the life of many young people. While the use of social media by youths is increasingly researched in the North, studies about youth in the South are missing. It therefore remains unclear how social media can be included in interventions that aim at informing young people in many countries of the global South about sexual and reproductive health. This paper presents findings of a mixed-methods study of young people's user behaviour on the internet and specifically of social media as a platform for sexual health promotion in Tanzania. The study used questionnaires with 60 adolescents and in-depth interviews with eight students aged 15 to 19 years in Dar es Salaam, and in Mtwara, Southern Tanzania. Findings show that youth in Dar es Salaam and Mtwara access the internet mainly through mobile phones. Facebook is by far the most popular internet site. Adolescents highlighted their interest in reproductive and sexual health messages and updates being delivered through humorous posts, links and clips, as well as by youth role models like music stars and actors that are entertaining and reflect up-to-date trends of modern youth culture. PMID:24908469

  8. A resource of vectors and ES cells for targeted deletion of microRNAs in mice.

    PubMed

    Prosser, Haydn M; Koike-Yusa, Hiroko; Cooper, James D; Law, Frances C; Bradley, Allan

    2011-08-07

    The 21-23 nucleotide, single-stranded RNAs classified as microRNAs (miRNA) perform fundamental roles in diverse cellular and developmental processes. In contrast to the situation for protein-coding genes, no public resource of miRNA mouse mutant alleles exists. Here we describe a collection of 428 miRNA targeting vectors covering 476 of the miRNA genes annotated in the miRBase registry. Using these vectors, we generated a library of highly germline-transmissible C57BL/6N mouse embryonic stem (ES) cell clones harboring targeted deletions for 392 miRNA genes. For most of these targeted clones, chimerism and germline transmission can be scored through a coat color marker. The targeted alleles have been designed to be adaptable research tools that can be efficiently altered by recombinase-mediated cassette exchange to create reporter, conditional and other allelic variants. This miRNA knockout (mirKO) resource can be searched electronically and is available from ES cell repositories for distribution to the scientific community.

  9. Mouse ES-cell-based functional assay to evaluate mutations in BRCA2

    PubMed Central

    Kuznetsov, Sergey G.; Liu, Pentao; Sharan, Shyam K.

    2009-01-01

    Individuals with mutations in breast cancer susceptibility genes, BRCA1 and BRCA2 have up to 80% risk of developing breast cancer by the age of 70. Sequencing based genetic tests are now available to identify mutation carriers in effort to reduce mortality through prevention and early diagnosis. However, lack of a suitable functional assay hinders risk assessment of more than 1900 BRCA1 and BRCA2 variants in the Breast Cancer Information Core database that do not clearly disrupt the gene product. We have established a simple, versatile and reliable assay to test for functional significance of mutations in BRCA2 using mouse embryonic stem cells (ES-cells) and bacterial artificial chromosomes (BACs) and have used it to classify 17 sequence variants. The assay is based on the ability of human BRCA2 to complement the loss of endogenous Brca2 in mouse ES-cells. This technique may also serve as a paradigm for functional analysis of mutations found in other human disease genes. PMID:18607349

  10. Casualty Risk Assessment Controlled Re-Entry of EPS - Ariane 5ES - ATV Mission

    NASA Astrophysics Data System (ADS)

    Arnal, M.-H.; Laine, N.; Aussilhou, C.

    2012-01-01

    To fulfil its mission of compliance check to the French Space Operations Act, CNES has developed ELECTRA© tool in order to estimate casualty risk induced by a space activity (like rocket launch, controlled or un-controlled re-entry on Earth of a space object). This article describes the application of such a tool for the EPS controlled re-entry during the second Ariane 5E/S flight (Johannes Kepler mission has been launched in February 2011). EPS is the Ariane 5E/S upper composite which is de-orbited from a 260 km circular orbit after its main mission (release of the Automated Transfer Vehicle - ATV). After a brief description of the launcher, the ATV-mission and a description of all the failure cases taken into account in the mission design (which leads to "back-up scenarios" into the flight software program), the article will describe the steps which lead to the casualty risk assessment (in case of failure) with ELECTRA©. In particular, the presence on board of two propulsive means of de-orbiting (main engine of EPS, and 4 ACS longitudinal nozzles in case of main engine failure or exhaustion) leads to a low remaining casualty risk.

  11. ES-3100: A New Generation Shipping Container for Bulk Highly Enriched Uranium and Other Fissile Materials

    SciTech Connect

    Arbital, J.G.; Byington, G.A.; Tousley, D.R.

    2004-07-01

    The U.S. Department of Energy (DOE) National Nuclear Security Administration (NNSA) is shipping bulk quantities of surplus fissile materials, primarily highly enriched uranium (HEU), over the next 15 to 20 years for disposition purposes. The U.S. Department of Transportation (DOT) specification 6M container is the package of choice for most of these shipments. However, the 6M does not conform to the Type B packaging requirements in the ''Code of Federal Regulations'' (10CFR71) and, for that reason, is being phased out for use in the secure transportation system of DOE. BWXT Y-12 is currently developing a package to replace the DOT 6M container for HEU disposition shipping campaigns. The new package is based on state-of-the-art, proven, and patented insulation technologies that have been successfully applied in the design of other packages. The new package, designated the ES-3100, will have a 50% greater capacity for HEU than the 6M and will be easier to use. Engineering analysis on the new package includes detailed dynamic impact finite element analysis (FEA). This analysis gives the ES-3100 a high probability of complying with regulatory requirements.

  12. Basic analysis of climate and urban bioclimate of Dar es Salaam, Tanzania

    NASA Astrophysics Data System (ADS)

    Ndetto, Emmanuel L.; Matzarakis, Andreas

    2013-10-01

    Better understanding of urban microclimate and bioclimate of any city is imperative today when the world is constrained by both urbanisation and global climate change. Urbanisation generally triggers changes in land cover and hence influencing the urban local climate. Dar es Salaam city in Tanzania is one of the fast growing cities. Assessment of its urban climate and the human biometeorological conditions was done using the easily available synoptic meteorological data covering the period 2001-2011. In particular, the physiologically equivalent temperature (PET) was calculated using the RayMan software and results reveal that the afternoon period from December to February (DJF season) is relatively the most thermal stressful period to human beings in Dar es Salaam where PET values of above 35 °C were found. Additionally, the diurnal cycle of the individual meteorological elements that influence the PET index were analysed and found that air temperature of 30-35 °C dominate the afternoon period from 12:00 to 15:00 hours local standard time at about 60 % of occurrence. The current results, though considered as preliminary to the ongoing urban climate study in the city, provide an insight on how urban climate research is of significant importance in providing useful climatic information for ensuring quality of life and wellbeing of city dwellers.

  13. COST-ES0601: Advances in homogenisation methods of climate series: an integrated approach (HOME)

    NASA Astrophysics Data System (ADS)

    Mestre, O.; Auer, I.; Venema, V.; Stepanek, P.; Szentimrey, T.; Grimvall, A.; Aguilar, E.

    2009-04-01

    The COST Action ES0601: Advances in homogenisation methods of climate series: an integrated approach is nearing the end of its second year of life. The action is intended to provide the best possible tools for the homogenization of time series to the climate research community. The involved scientists have done remarkable progress since COST Action ES0601 was launched (see www.homogenisation.org). HOME has started with a literature review and a survey to the research community to identify those climatic elements and homogenisation techniques to be considered during the action. This allowed the preparation of the benchmark monthly dataset to be used during the remaining time of the action. This monthly benchmark contains real temperature and precipitation data (with real inhomogeneities), as well as synthetic and surrogate networks, including artificially produced missing values, outliers, local trends and break inhomogeneities which are inserted at the usual rate, size and distribution found in actual networks. The location of the outliers and change points is undisclosed to the HOME scientists, who are, at present, applying different homogenisation approaches and uploading the results, to analyse the performances of their techniques. Everyone who works on the homogenization of climate data is cordially invited to join this exercise. HOME is also working on the production of a daily benchmark dataset, to reproduce the experiment described above, but in a lower temporal resolution, and on the preparation of freely available homogenization tools, including the best performing approaches.

  14. Excretory/secretory antigens (ES) from in-vitro cultures of Taenia crassiceps cysticerci, and use of an anti-ES monoclonal antibody for antigen detection in samples of cerebrospinal fluid from patients with neurocysticercosis.

    PubMed

    Espíndola, N M; Vaz, A J; Pardini, A X; Fernandes, I

    2002-06-01

    Antigens were obtained from cysticerci of the ORF strain of Taenia crassiceps, by culture of cysts in protein-free hybridoma medium (PFHM). Budding of new vesicles was observed after 24-48 h. Excretory/secretory (ES) antigens (peptides of <20 kDa) were recovered in the medium after culture for 48 h. SDS-PAGE analysis of vesicular-fluid (VF) antigens (obtained by rupturing T. crassiceps cysticerci in PFHM) and the ES antigens indicated partial homology between the two preparations. ES peptides of 18- and 14-kDa were recognized by polyclonal antibodies produced in rabbits immunized either with the VF antigens or with a total-antigen preparation of T. solium cysticerci. Antibodies present in samples of serum or cerebrospinal fluid (CSF) from patients with neurocysticercosis also reacted with ES peptides. An anti-ES monoclonal antibody detected antigens in the CSF from 10 patients with neurocysticercosis, showing the antigenic homology of the ES antigens with those of T. solium cysticerci in human infections.

  15. Historique en grandes enjambées de la thermodynamique de l'équilibre

    NASA Astrophysics Data System (ADS)

    Hertz, J.

    2004-12-01

    La Thermodynamique, une science totalement nouvelle au XIXème siècle, a germé en France en contrepoint des idées du siècle des Lumières, dans le milieu particulier des anciens élèves de l’Ecole Polytechnique, officiers supérieurs formés pour l’armée républicaine ou napoléonienne, mais qui ne trouvaient plus leur place dans l’armée de la Restauration. Ils se convertissaient en ingénieurs civils des métiers industriels en pleine expansion, comme le développement de la machine à vapeur ou des chemins de fer. La plupart d’entre eux, plutôt libre-penseurs, adhéraient aux idées scientistes du « positivisme », véhiculées dans les Loges de la Franc-Maçonnerie du Grand Orient de France et plus particulièrement dans les cercles Saint-Simoniens, premiers adeptes du socialisme industriel. C’est ainsi que naquit en 1824, dans le cerveau subtil mais brouillon de Sadi Carnot toute la vision illuminée de cette science nouvelle, incompréhensible pour ses contemporains. Elle ressuscita en 1834 sous la plume d’un Emile Clapeyron qui avait pris conscience de l’immensité de l’œuvre de Sadi Carnot. Mais le rappel de Clapeyron demeura également sans écho pendant dix années. Le réveil de la Thermodynamique se fera désormais hors de France par des hommes de grande pratique religieuse et généralement protestants. C’est ainsi que William Thomson en Ecosse et Rudolph Clausius, venu de Prusse, achevèrent l’œuvre de leurs deux prédécesseurs et que la Thermodynamique mécano-thermique fut définitivement établie en 1864. La thermodynamique chimique peut être attribuée à un seul génie mathématicien, Josiah Willard Gibbs qui travaillait tout seul au Yale College de New-Haven, dans le Connecticut, et rédigea sa nouvelle théorie entre 1875 et 1878. Enfin l’interprétation statistique du second principe sera l’œuvre en 1877 d’un Autrichien, Ludwig Boltzmann, homme génial mais fragile qui eut le temps d’insuffler ses idées

  16. Modeling Urban Growth Spatial Dynamics: Case studies of Addis Ababa and Dar es Salaam

    NASA Astrophysics Data System (ADS)

    Buchta, Katja; Abo El Wafa, Hany; Printz, Andreas; Pauleit, Stephan

    2013-04-01

    Rapid urbanization, and consequently, the dramatic spatial expansion of mostly informal urban areas increases the vulnerability of African cities to the effects of climate change such as sea level rise, more frequent flooding, droughts and heat waves. The EU FP 7 funded project CLUVA (Climate Change and Urban Vulnerability in Africa, www.cluva.eu) aims to develop strategies for minimizing the risks of natural hazards caused by climate change and to improve the coping capacity of African cities. Green infrastructure may play a particular role in climate change adaptation by providing ecosystem services for flood protection, stormwater retention, heat island moderation and provision of food and fuel wood. In this context, a major challenge is to gain a better understanding of the spatial and temporal dynamics of the cities and how these impact on green infrastructure and hence their vulnerability. Urban growth scenarios for two African cities, namely Addis Ababa, Ethiopia and Dar es Salaam, Tanzania, were developed based on a characterization of their urban morphology. A population growth driven - GIS based - disaggregation modeling approach was applied. Major impact factors influencing the urban dynamics were identified both from literature and interviews with local experts. Location based factors including proximity to road infrastructure and accessibility, and environmental factors including slope, surface and flood risk areas showed a particular impact on urban growth patterns. In Addis Ababa and Dar es Salaam, population density scenarios were modeled comparing two housing development strategies. Results showed that a densification scenario significantly decreases the loss of agricultural and green areas such as forests, bushland and sports grounds. In Dar es Salaam, the scenario of planned new settlements with a population density of max. 350 persons per hectare would lead until 2025 to a loss of agricultural land (-10.1%) and green areas (-6.6%). On the other

  17. Wnt/ß-catenin signalling and the dynamics of fate decisions in early mouse embryos and embryonic stem (ES) cells.

    PubMed

    Muñoz-Descalzo, Silvia; Hadjantonakis, Anna-Katerina; Arias, Alfonso Martinez

    2015-12-01

    Wnt/ß-catenin signalling is a widespread cell signalling pathway with multiple roles during vertebrate development. In mouse embryonic stem (mES) cells, there is a dual role for ß-catenin: it promotes differentiation when activated as part of the Wnt/ß-catenin signalling pathway, and promotes stable pluripotency independently of signalling. Although mES cells resemble the preimplantation epiblast progenitors, the first requirement for Wnt/ß-catenin signalling during mouse development has been reported at implantation [1,2]. The relationship between ß-catenin and pluripotency and that of mES cells with epiblast progenitors suggests that ß-catenin might have a functional role during preimplantation development. Here we summarize the expression and function of Wnt/ß-catenin signalling elements during the early stages of mouse development and consider the reasons why the requirement in ES cells do not reflect the embryo.

  18. The immunomodulatory parasitic worm product ES-62 reduces lupus-associated accelerated atherosclerosis in a mouse model

    PubMed Central

    Aprahamian, Tamar R.; Zhong, Xuemei; Amir, Shahzada; Binder, Christoph J.; Chiang, Lo-Ku; Al-Riyami, Lamyaa; Gharakhanian, Raffi; Harnett, Margaret M.; Harnett, William; Rifkin, Ian R.

    2015-01-01

    ES-62 is an anti-inflammatory phosphorylcholine-containing glycoprotein secreted by the filarial nematode Acanthocheilonema viteae. Accelerated atherosclerosis frequently occurs in systemic lupus erythematosus (SLE), resulting in substantial cardiovascular morbidity and mortality. We examined the effects of ES-62 in the gld.apoE−/− mouse model of this condition. Treatment with ES-62 did not substantially modulate renal pathology but caused decreased anti-nuclear autoantibody levels. Moreover, a striking 60% reduction in aortic atherosclerotic lesions was observed, with an associated decrease in macrophages and fibrosis. We believe that these latter findings constitute the first example of a defined parasitic worm product with therapeutic potential in atherosclerosis: ES-62-based drugs may represent a novel approach to control accelerated atherosclerosis in SLE. PMID:25666929

  19. Transient conformational remodeling of folding proteins by GroES-individually and in concert with GroEL.

    PubMed

    Moparthi, Satish Babu; Sjölander, Daniel; Villebeck, Laila; Jonsson, Bengt-Harald; Hammarström, Per; Carlsson, Uno

    2013-01-01

    The commonly accepted dogma of the bacterial GroE chaperonin system entails protein folding mediated by cycles of several ATP-dependent sequential steps where GroEL interacts with the folding client protein. In contrast, we herein report GroES-mediated dynamic remodeling (expansion and compression) of two different protein substrates during folding: the endogenous substrate MreB and carbonic anhydrase (HCAII), a well-characterized protein folding model. GroES was also found to influence GroEL binding induced unfolding and compression of the client protein underlining the synergistic activity of both chaperonins, even in the absence of ATP. This previously unidentified activity by GroES should have important implications for understanding the chaperonin mechanism and cellular stress response. Our findings necessitate a revision of the GroEL/ES mechanism. PMID:24386013

  20. Study on glyco-modification of endostatin-derived synthetic peptide endostatin2 (ES2) by soluble chitooligosaccharide.

    PubMed

    Yu, Yang; Sun, Feng; Zhang, Congcong; Wang, Zhendong; Liu, Jinfeng; Tan, Haining

    2016-12-10

    Soluble O-(2-hydroxyl)propyl-3-trimethyl ammonium chitooligosaccharide chloride (HTCOSC) was covalently conjugated to the 11-amino-acid peptide derived from amino terminus of endostatin (endostatin2, ES2, IVRRADRAAVP) to overcome its poor stability, low cell affinity and instable activity. Nuclear magnetic resonance spectroscopy and mass spectrometry was used to study the structure and molecular weight information. The anti-angiogenic activities were evaluated using cell counting Kit-8 assay, flow cytometry assay, wounding migration assay, transwell migration assay, chicken chorioallantoic membrane (CAM) assay and zebra fish angiogenesis assay. In contrast with ES2, the novel carbohydrate-polymer HTCOSC-ES2 displayed improved heat stability, higher cell affinity, better inhibition on endothelial cell proliferation, tube formation, 2-dimensional and 3-dimensional migration in vitro. According to the evaluation in CAM and zebra fish, HTCOSC-ES2 also displayed better anti-angiogenic activity than ES2 in vivo. These results indicate that HTCOSC has good properties as potential candidate for protein/peptide modifier and HTCOSC-ES2 has good potential in angiogenesis related diseases treatment. PMID:27577911

  1. Nuées ardentes of 22 November 1994 at Merapi volcano, Java, Indonesia

    USGS Publications Warehouse

    Abdurachman, E.K.; Bourdier, J.-L.; Voight, B.

    2000-01-01

    Nuées ardentes associated with dome collapse on 22 November 1994, at Merapi volcano traveled to the south–southwest as far as 6.5 km, and collectively accumulated roughly 2.5–3 million cubic meters of deposits. The damaged area comprises 9.5 km2 and is covered by two nuée ardente facies, a conventional “Merapi-type”, valley-fill block-and-ash flow facies and a pyroclastic surge facies. The proximal deposits reflect the accumulation of dozens of nuées ardentes, with many subsidiary flow units. The distal deposits are more simply organized, as only a few individual events reached to distances >3.5 km. The stratigraphic relationships north of Turgo hill indicate that the surge deposits are a facies of particularly mobile nuées ardentes that also deposited channeled block-and-ash flow facies. They further suggest that the surge facies beyond the channel margins correlate laterally with a finer-grained sublayer locally developed at the base of the block-and-ash flow facies. Eyewitness reports suggest that the emplacement of the block-and-ash flow facies in the distal part of the Boyong river may have followed, by a short time interval, the destruction and deposition of the surge facies at Turgo village. The stratigraphy is in accord with the eyewitness reports. The surge facies was emplaced by a dilute surge current, detached from the same dome-collapse nuée ardente that, as a separate flow unit, subsequently emplaced the distal block-and-ash deposit in the Boyong valley. The detachment occurred at higher elevations, likely at or above the slope break at about 2000 m elevation. This flow separation enabled the surge current to shortcut over the landscape and to emplace its deposit even as the block-and-ash flow continued its tortuous southward movement in the Boyong channel. Dome-collapse nuée ardente activity formed the bulk of the eruption, which was accompanied by virtually no significant vertical summit explosive activity.

  2. Study of delayed fission of the isotopes of Bk, Es, and Md

    SciTech Connect

    Gangrskii, Y.P.; Miller, M.B.; Mikhailov, L.V.; Kharisov, I.F.

    1980-02-01

    We have measured the probabilities of delayed fission in electron capture for the nuclei /sup 240,242/Bk, /sup 244,246,248/Es, and /sup 248,250/Md. The data are analyzed by means of analytical expressions obtained in the work which explicitly relate the probability of delayed fission (in electron capture or ..beta../sup -/ decay) to the parameters of a two-humped fission barrier. As a result of the analysis the fission barriers are evaluated for the corresponding daughter nuclei: the isotopes of Cm, Cf, and Fm. According to the estimates the height of the fission barrier for the group of nuclei investigated is close to the value 6 MeV and does not decrease appreciably with increase of the Z of the nucleus or with removal from the ..beta..-stability band.

  3. Urban mosquitoes, situational publics, and the pursuit of interspecies separation in Dar es Salaam

    PubMed Central

    KELLY, ANN H.; LEZAUN, JAVIER

    2014-01-01

    Recent work in anthropology points to the recognition of multispecies entanglements as the grounds for a more ethical politics. In this article, we examine efforts to control mosquitoes in Dar es Salaam, Tanzania, as an example of the laborious tasks of disentanglement that characterize public health interventions. The mosquito surveillance and larval elimination practices of an urban malaria control program offer an opportunity to observe how efforts to create distance between species relate to the physical and civic textures of the city. Seen in the particular context of the contemporary African metropolis, the work of public health appears less a matter of control than a commitment to constant urban maintenance and political mobilization. PMID:25429167

  4. ?`Es necesario calcular detalladamente funciones de partición atómicas?

    NASA Astrophysics Data System (ADS)

    Milone, L. A.; Merlo, D. C.

    Basándonos en extensos y precisos cómputos de funciones de partición realizados por nosotros para distintos átomos, se muestra que en el cálculo u obtención de ciertas magnitudes (notablemente la presión electrónica, la abundancia de un elemento deducida a partir de un estado fuertemente ionizado, etc.) el error porcentual que se comete es pequeño (inferior a 1 %) si se adopta, como valor de la función de partición, el peso estadístico del término correspondiente al estado fundamental del átomo. Esta notable simplificación acelera el cálculo, por ejemplo, de un modelo de atmósfera estelar, sin disminuir la precisión de los resultados.

  5. Outreach with Team eS Through Science Festivals and Interactive Art Installations

    NASA Astrophysics Data System (ADS)

    Yoho, Amanda; Starkman, Glenn

    2014-03-01

    The Team eS project aims to acclimate (pre)teens to scientific concepts subtly, with fun, accessible, and engaging art and activities hosted at public community festivals, online at a dedicated website, and using social media. Our festivals will be centered around an interactive art installation inspired by a scientific concept. We hope to provide a positive experience inspired by science that these teens can reflect upon when encountering similar concepts in the future, especially in settings like a classroom where fear and anxiety can cloud interest or performance. We want to empower teens to not feel lost or out of the loop - we want to remove the fear of facing science.

  6. Understanding Household Behavioral Risk Factors for Diarrheal Disease in Dar es Salaam: A Photovoice Community Assessment

    PubMed Central

    Badowski, Natalie; Castro, Cynthia M.; Montgomery, Maggie; Pickering, Amy J.; Mamuya, Simon; Davis, Jennifer

    2011-01-01

    Whereas Tanzania has seen considerable improvements in water and sanitation infrastructure over the past 20 years, the country still faces high rates of childhood morbidity from diarrheal diseases. This study utilized a qualitative, cross-sectional, modified Photovoice method to capture daily activities of Dar es Salaam mothers. A total of 127 photographs from 13 households were examined, and 13 interviews were conducted with household mothers. The photographs and interviews revealed insufficient hand washing procedures, unsafe disposal of wastewater, uncovered household drinking water containers, a lack of water treatment prior to consumption, and inappropriate toilets for use by small children. The interviews revealed that mothers were aware and knowledgeable of the risks of certain household practices and understood safer alternatives, yet were restricted by the perceived impracticality and financial constraints to make changes. The results draw attention to the real economic and behavioral challenges faced in reducing the spread of disease. PMID:21969836

  7. Climate change induced risk analysis of Dar es Salaam city (Tanzania)

    NASA Astrophysics Data System (ADS)

    Topa, Maria Elena; Herslund, Lise; Cavan, Gina; Printz, Andreas; Simonis, Ingo; Bucchignani, Edoardo; Jean-Baptiste, Nathalie; Hellevik, Siri; Johns, Regina; Kibassa, Deusdedit; Kweka, Clara; Magina, Fredrick; Mangula, Alpha; Mbuya, Elinorata; Uhinga, Guido; Kassenga, Gabriel; Kyessi, Alphonce; Shemdoe, Riziki; Kombe, Wilbard

    2013-04-01

    CLUVA (CLimate change and Urban Vulnerability in Africa; http://www.cluva.eu/) is a 3 years project, funded by the European Commission in 2010. The main objective of CLUVA is to develop context-centered methods and knowledge to be applied to African cities to assess vulnerabilities and increase knowledge on managing climate related risks. The project estimates the impacts of climate changes in the next 40 years at urban scale and downscales IPCC climate projections to evaluate specific threats to selected African test cities. These are mainly from floods, sea-level rise, droughts, heat waves, and desertification. The project evaluates and links: social vulnerability; urban green structures and ecosystem services; urban-rural interfaces; vulnerability of urban built environment and lifelines; and related institutional and governance dimensions of adaptation. The multi-scale and multi-disciplinary qualitative, quantitative and probabilistic approach of CLUVA is currently being applied to selected African test cities (Addis Ababa - Ethiopia; Dar es Salaam - Tanzania; Douala - Cameroun; Ouagadougou - Burkina Faso; St. Louis - Senegal). In particular, the poster will present preliminary findings for the Dar es Salaam case study. Dar es Salaam, which is Tanzania's largest coastal city, is exposed to floods, coastal erosion, droughts and heat waves, and highly vulnerable to impacts as a result of ineffective urban planning (about 70% unplanned settlements), poverty and lack of basic infrastructure (e.g. lack of or poor quality storm water drainage systems). Climate change could exacerbate the current situation increasing hazard-exposure alongside the impacts of development pressures which act to increase urban vulnerability for example because of informal (unregulated) urbanization. The CLUVA research team - composed of climate and environmental scientists, risk management experts, urban planners and social scientists from both European and African institutions - has

  8. POSSIBLE TRANSIT TIMING VARIATIONS OF THE TrES-3 PLANETARY SYSTEM

    SciTech Connect

    Jiang, Ing-Guey; Wu, Yu-Ting; Chien, Ping; Lin, Yi-Ling; Chen, Hong-Yu; Hu, Juei-Hwa; Yeh, Li-Chin; Thakur, Parijat; Sun Zhao; Ji Jianghui

    2013-03-15

    Five newly observed transit light curves of the TrES-3 planetary system are presented. Together with other light-curve data from the literature, 23 transit light curves in total, which cover an overall timescale of 911 epochs, have been analyzed through a standard procedure. From these observational data, the system's orbital parameters are determined and possible transit timing variations (TTVs) are investigated. Given that a null TTV produces a fit with reduced {chi}{sup 2} = 1.52, our results agree with previous work, that TTVs might not exist in these data. However, a one-frequency oscillating TTV model, giving a fit with a reduced {chi}{sup 2} = 0.93, does possess a statistically higher probability. It is thus concluded that future observations and dynamical simulations for this planetary system will be very important.

  9. ES12; The 24th Annual Workshop on Recent Developments in Electronic Structure Theory

    SciTech Connect

    Holzwarth, Natalie; Thonhauser, Timo; Salam, Akbar

    2012-06-29

    ES12: The 24th Annual Workshop on Recent Developments in Electronic Structure Theory was held June 5-8, 2012 at Wake Forest University in Winston-Salem, NC 27109. The program consisted of 24 oral presentations, 70 posters, and 2 panel discussions. The attendance of the Workshop was comparable to or larger than previous workshops and participation was impressively diverse. The 136 participants came from all over the world and included undergraduate students, graduate students, postdoctoral researchers, and senior scientists. The general assessment of the Workshop was extremely positive in terms of the high level of scientific presentations and discussions, and in terms of the schedule, accommodations, and affordability of the meeting.

  10. Ethanol and Methanol Can Improve Huperzine A Production from Endophytic Colletotrichum gloeosporioides ES026

    PubMed Central

    Zhao, Xin-Mei; Wang, Zhang-Qian; Shu, Shao-Hua; Wang, Wen-Juan; Xu, Hai-Jie; Ahn, Young-Joon; Wang, Mo; Hu, Xuebo

    2013-01-01

    Huperzine A (HupA) is a plant alkaloid that is of great interest as a therapeutic candidate for the treatment of Alzheimer's disease. However, the current production of HupA from plants in large quantity is unsustainable because the plant resource is scarce and the content of HupA in plants is extremely low. Surprisingly, this compound was recently found to be produced by various endophytic fungi, which are much more controllable than the plants due to simpler genetics and ease of manipulation. However, it might be due to the innate properties of endophytic symbiosis, that production of this chemical in large quantity from endophytes has not yet been put into practice. Endophytic Colletotrichum gloeosporioides ES026 was previously isolated from a HupA producing plant and the fungi also proved to produce HupA. In this study, various fermentation conditions were tried to optimize the production of HupA from C. gloeosporioides ES026. Optimization of these parameters resulted in a 25.58% increase in HupA yield. Potato extracts supplemented with glucose or sucrose but not maltose facilitated HupA producing from the fungi. A final concentration of 0.5–2% ethanol stimulated the growth of fungi while methanol with the same treatment slightly inhibited the growth. However, both methanol and ethanol greatly increased the HupA production with the highest yield of HupA (51.89% increment) coming from ethanol treatment. Further analysis showed that both ethanol and methanol were strong inducers of HupA production, while ethanol was partially used as a carbon source during fermentation. It was noticed that the color of that ethanol treated mycelia gradually became dark while methanol treated ones stayed grey during fermentation. The present study sheds light on the importance of optimizing the fermentation process, which, combined with effective inducers, maximizes production of chemicals of important economic interest from endophytic fungi. PMID:23613930

  11. Extended Light in E/S0 Galaxies and Implications for Disk Rebirth

    NASA Astrophysics Data System (ADS)

    Moffett, Amanda J.; Kannappan, S. J.; Laine, S.; Wei, L. H.; Baker, A. J.; Impey, C. D.

    2010-01-01

    The recent discovery of extended ultraviolet (XUV) disks around a large fraction of late-type galaxies provides evidence for unexpectedly large-scale disk building at recent epochs. Combining GALEX UV observations with deep optical and Spitzer IR imaging, we search for XUV disks in a sample of nearby low-to-intermediate mass E/S0 galaxies to explore evidence for disk rebuilding after mergers. Preliminary visual classification yields ten XUV-disk candidates from the full sample of 30, intriguingly similar to the 30% frequency for late-type galaxies. These XUV candidates occur at a wide range of masses and on both the red and blue sequences in color vs. stellar mass, indicating a possible association with processes like gas accretion and/or galaxy interactions that would affect the galaxy population broadly. We go on to apply the quantitative Type 1 and Type 2 XUV-disk definitions to this sample and identify multiple Type 1 XUVs, i.e., galaxies with UV structure beyond the expected star formation threshold. We also find several galaxies that come close to satisfying the Type 2 definition, but that definition proves problematic to apply to this sample: the NUV-derived star formation threshold radii for our E/S0s often lie inside the 80% Ks-band light (K80) radii, violating an implicit assumption of the Type 2 definition, or lie outside but not as far as the definition requires. Nonetheless, we identify otherwise Type 2-like galaxies ("modified Type 2 XUVs") that have higher star formation rates and bluer FUV - NUV colors than Type 1 XUVs in the sample. We propose that Type 1 XUVs may reflect early or inefficient stages of star formation, while modified Type 2 XUVs perhaps reflect inside-out disk regrowth.

  12. Extended Light in E/S0 Galaxies and Implications for Disk Rebirth

    NASA Astrophysics Data System (ADS)

    Moffett, A. J.; Kannappan, S. J.; Laine, S.; Wei, L. H.; Baker, A. J.; Impey, C. D.

    2010-06-01

    The recent discovery of extended ultraviolet (XUV) disks around a large fraction of late-type galaxies provides evidence for unexpectedly large-scale disk building at recent epochs. Combining GALEX UV observations with deep optical and Spitzer IR imaging, we search for XUV disks in a sample of nearby low-to-intermediate mass E/S0 galaxies to explore evidence for disk rebuilding after mergers. Preliminary visual classification yields ten XUV-disk candidates from the full sample of 30, intriguingly similar to the ˜30% frequency for late-type galaxies. These XUV candidates occur at a wide range of masses and on both the red and blue sequences in color vs. stellar mass, indicating a possible association with processes like gas accretion and/or galaxy interactions that would affect the galaxy population broadly. We go on to apply the quantitative Type 1 and Type 2 XUV-disk definitions to a nine-galaxy subsample analyzed in detail. For this subsample, six of the nine are Type 1 XUVs, i.e., galaxies with UV structure beyond the expected star formation threshold. The other three come close to satisfying the Type 2 definition, but that definition proves problematic to apply to this sample: the NUV-derived star formation threshold radii for our E/S0s often lie inside the 80% Ks-band light (K80) radii, violating an implicit assumption of the Type 2 definition, or lie outside but not as far as the definition requires. Nonetheless, the three otherwise Type 2-like galaxies ("modified Type 2 XUVs") have higher star formation rates and bluer FUV - NUV colors than the Type 1 XUVs in the sample. We propose that Type 1 XUVs may reflect early or inefficient stages of star formation, while modified Type 2 XUVs perhaps reflect inside-out disk regrowth.

  13. Last glacial maximum constraints on the Earth System model HadGEM2-ES

    NASA Astrophysics Data System (ADS)

    Hopcroft, Peter O.; Valdes, Paul J.

    2015-09-01

    We investigate the response of the atmospheric and land surface components of the CMIP5/AR5 Earth System model HadGEM2-ES to pre-industrial (PI: AD 1860) and last glacial maximum (LGM: 21 kyr) boundary conditions. HadGEM2-ES comprises atmosphere, ocean and sea-ice components which are interactively coupled to representations of the carbon cycle, aerosols including mineral dust and tropospheric chemistry. In this study, we focus on the atmosphere-only model HadGEM2-A coupled to terrestrial carbon cycle and aerosol models. This configuration is forced with monthly sea surface temperature and sea-ice fields from equivalent coupled simulations with an older version of the Hadley Centre model, HadCM3. HadGEM2-A simulates extreme cooling over northern continents and nearly complete die back of vegetation in Asia, giving a poor representation of the LGM environment compared with reconstructions of surface temperatures and biome distributions. The model also performs significantly worse for the LGM in comparison with its precursor AR4 model HadCM3M2. Detailed analysis shows that the major factor behind the vegetation die off in HadGEM2-A is a subtle change to the temperature dependence of leaf mortality within the phenology model of HadGEM2. This impacts on both snow-vegetation albedo and vegetation dynamics. A new set of parameters is tested for both the pre-industrial and LGM, showing much improved coverage of vegetation in both time periods, including an improved representation of the needle-leaf forest coverage in Siberia for the pre-industrial. The new parameters and the resulting changes in global vegetation distribution strongly impact the simulated loading of mineral dust, an important aerosol for the LGM. The climate response in an abrupt 4× pre-industrial CO2 simulation is also analysed and shows modest regional impacts on surface temperatures across the Boreal zone.

  14. Multiwavelength observations of the blazar 1ES 1011+496 in Spring 2008

    NASA Astrophysics Data System (ADS)

    Ahnen, M. L.; Ansoldi, S.; Antonelli, L. A.; Antoranz, P.; Babic, A.; Banerjee, B.; Bangale, P.; de Almeida, U. Barres; Barrio, J. A.; González, J. Becerra; Bednarek, W.; Bernardini, E.; Biasuzzi, B.; Biland, A.; Blanch, O.; Bonnefoy, S.; Bonnoli, G.; Borracci, F.; Bretz, T.; Carmona, E.; Carosi, A.; Chatterjee, A.; Clavero, R.; Colin, P.; Colombo, E.; Contreras, J. L.; Cortina, J.; Covino, S.; Da Vela, P.; Dazzi, F.; De Angelis, A.; De Caneva, G.; De Lotto, B.; de Oña Wilhelmi, E.; Mendez, C. Delgado; Pierro, F. Di; Prester, D. Dominis; Dorner, D.; Doro, M.; Einecke, S.; Elsaesser, D.; Fernández-Barral, A.; Fidalgo, D.; Fonseca, M. V.; Font, L.; Frantzen, K.; Fruck, C.; Galindo, D.; López, R. J. García; Garczarczyk, M.; Terrats, D. Garrido; Gaug, M.; Giammaria, P.; (Eisenacher), D. Glawion; Godinović, N.; Muñoz, A. González; Guberman, D.; Hanabata, Y.; Hayashida, M.; Herrera, J.; Hose, J.; Hrupec, D.; Hughes, G.; Idec, W.; Kodani, K.; Konno, Y.; Kubo, H.; Kushida, J.; Barbera, A. La; Lelas, D.; Lindfors, E.; Lombardi, S.; Longo, F.; López, M.; López-Coto, R.; López-Oramas, A.; Lorenz, E.; Majumdar, P.; Makariev, M.; Mallot, K.; Maneva, G.; Manganaro, M.; Mannheim, K.; Maraschi, L.; Marcote, B.; Mariotti, M.; Martínez, M.; Mazin, D.; Menzel, U.; Miranda, J. M.; Mirzoyan, R.; Moralejo, A.; Nakajima, D.; Neustroev, V.; Niedzwiecki, A.; Rosillo, M. Nievas; Nilsson, K.; Nishijima, K.; Noda, K.; Orito, R.; Overkemping, A.; Paiano, S.; Palacio, J.; Palatiello, M.; Paneque, D.; Paoletti, R.; Paredes, J. M.; Paredes-Fortuny, X.; Persic, M.; Poutanen, J.; Moroni, P. G. Prada; Prandini, E.; Puljak, I.; Reinthal, R.; Rhode, W.; Ribó, M.; Rico, J.; Garcia, J. Rodriguez; Rügamer, S.; Saito, T.; Satalecka, K.; Scapin, V.; Schultz, C.; Schweizer, T.; Shore, S. N.; Sillanpää, A.; Sitarek, J.; Snidaric, I.; Sobczynska, D.; Stamerra, A.; Steinbring, T.; Strzys, M.; Takalo, L.; Takami, H.; Tavecchio, F.; Temnikov, P.; Terzić, T.; Tescaro, D.; Teshima, M.; Thaele, J.; Torres, D. F.; Toyama, T.; Treves, A.; Verguilov, V.; Vovk, I.; Ward, J. E.; Will, M.; Wu, M. H.; Zanin, R.; Lucarelli, F.; Pittori, C.; Vercellone, S.; Berdyugin, A.; Carini, M. T.; Lähteenmäki, A.; Pasanen, M.; Pease, A.; Sainio, J.; Tornikoski, M.; Walters, R.

    2016-07-01

    The BL Lac object 1ES 1011+496 was discovered at very high energy (VHE, E > 100GeV) γ-rays by Major Atmospheric Gamma-ray Imaging Cherenkov (MAGIC) in Spring 2007. Before that the source was little studied in different wavelengths. Therefore, a multiwavelength (MWL) campaign was organized in Spring 2008. Along MAGIC, the MWL campaign included the Metsähovi Radio Observatory, Bell and Kungliga Vetenskapsakademien (KVA) optical telescopes and the Swift and AGILE satellites. MAGIC observations span from 2008 March to May for a total of 27.9 h, of which 19.4 h remained after quality cuts. The light curve showed no significant variability yielding an integral flux above 200 GeV of (1.3 ± 0.3) × 10-11 photons cm-2 s-1. The differential VHE spectrum could be described with a power-law function with a spectral index of 3.3 ± 0.4. Both results were similar to those obtained during the discovery. Swift X-ray Telescope observations revealed an X-ray flare, characterized by a harder-when-brighter trend, as is typical for high synchrotron peak BL Lac objects (HBL). Strong optical variability was found during the campaign, but no conclusion on the connection between the optical and VHE γ-ray bands could be drawn. The contemporaneous spectral energy distribution shows a synchrotron-dominated source, unlike concluded in previous work based on non-simultaneous data, and is well described by a standard one-zone synchrotron self-Compton model. We also performed a study on the source classification. While the optical and X-ray data taken during our campaign show typical characteristics of an HBL, we suggest, based on archival data, that 1ES 1011+496 is actually a borderline case between intermediate and high synchrotron peak frequency BL Lac objects.

  15. Six-year optical monitoring of the BL Lacertae object 1ES 0806+52.4

    SciTech Connect

    Man, Zhongyi; Zhang, Xiaoyuan; Wu, Jianghua; Zhou, Xu; Yuan, Qirong

    2014-12-01

    We present the results of the first systematic long-term multicolor optical monitoring of the BL Lacertae object 1ES 0806+52.4. The monitoring was performed in multiple passbands with a 60/90 cm Schmidt telescope from 2005 December to 2011 February. The overall brightness of this object decreased from 2005 December to 2008 December but was regained after that. A sharp outburst probably occurred around the end of our monitoring program. Overlapping the long-term trend are some short-term small-amplitude oscillations. No intranight variability was found in the object, which is in accordance with the historical observations before 2005. By investigating the color behavior, we found a strong bluer-when-brighter chromatism for the long-term variability of 1ES 0806+52.4. The total amplitudes at the c, i, and o bands are 1.18, 1.12, and 1.02 mag, respectively. The amplitudes tend to increase toward shorter wavelengths, which may be a major cause of the bluer-when-brighter chromatism. Such bluer-when-brighter chromatisms are also found in other blazars, such as S5 0716+714, OJ 287. The hard-X-ray data collected from the Swift/BAT archive was correlated with our optical data. No positive result was found, the reason for which may be that the hard-X-ray flux is a combination of the synchrotron and inverse Compton emission, but with different timescales and cadences under the leptonic synchrotron self-Compton model.

  16. Human Embryonic Stem Cell-Derived Mesenchymal Stroma Cells (hES-MSCs) Engraft In Vivo and Support Hematopoiesis without Suppressing Immune Function: Implications for Off-The Shelf ES-MSC Therapies

    PubMed Central

    Li, Ou; Tormin, Ariane; Sundberg, Berit; Hyllner, Johan; Le Blanc, Katarina; Scheding, Stefan

    2013-01-01

    Mesenchymal stroma cells (MSCs) have a high potential for novel cell therapy approaches in clinical transplantation. Commonly used bone marrow-derived MSCs (BM-MSCs), however, have a restricted proliferative capacity and cultures are difficult to standardize. Recently developed human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) might represent an alternative and unlimited source of hMSCs. We therefore compared human ES-cell-derived MSCs (hES-MP002.5 cells) to normal human bone marrow-derived MSCs (BM-MSCs). hES-MP002.5 cells had lower yet reasonable CFU-F capacity compared with BM-MSC (8±3 versus 29±13 CFU-F per 100 cells). Both cell types showed similar immunophenotypic properties, i.e. cells were positive for CD105, CD73, CD166, HLA-ABC, CD44, CD146, CD90, and negative for CD45, CD34, CD14, CD31, CD117, CD19, CD 271, SSEA-4 and HLA-DR. hES-MP002.5 cells, like BM-MSCs, could be differentiated into adipocytes, osteoblasts and chondrocytes in vitro. Neither hES-MP002.5 cells nor BM-MSCs homed to the bone marrow of immune-deficient NSG mice following intravenous transplantation, whereas intra-femoral transplantation into NSG mice resulted in engraftment for both cell types. In vitro long-term culture-initiating cell assays and in vivo co-transplantation experiments with cord blood CD34+ hematopoietic cells demonstrated furthermore that hES-MP002.5 cells, like BM-MSCs, possess potent stroma support function. In contrast to BM-MSCs, however, hES-MP002.5 cells showed no or only little activity in mixed lymphocyte cultures and phytohemagglutinin (PHA) lymphocyte stimulation assays. In summary, ES-cell derived MSCs might be an attractive unlimited source for stroma transplantation approaches without suppressing immune function. PMID:23383153

  17. MOR209/ES414, a Novel Bispecific Antibody Targeting PSMA for the Treatment of Metastatic Castration-Resistant Prostate Cancer.

    PubMed

    Hernandez-Hoyos, Gabriela; Sewell, Toddy; Bader, Robert; Bannink, Jeannette; Chenault, Ruth A; Daugherty, Mollie; Dasovich, Maria; Fang, Hang; Gottschalk, Rebecca; Kumer, John; Miller, Robert E; Ravikumar, Padma; Wiens, Jennifer; Algate, Paul A; Bienvenue, David; McMahan, Catherine J; Natarajan, Sateesh K; Gross, Jane A; Blankenship, John W

    2016-09-01

    Treatment of metastatic, castration-resistant prostate cancer (mCRPC) remains a highly unmet medical need and current therapies ultimately result in disease progression. Immunotherapy is a rapidly growing approach for treatment of cancer but has shown limited success to date in the treatment of mCRPC. We have developed a novel humanized bispecific antibody, MOR209/ES414, built on the ADAPTIR (modular protein technology) platform, to redirect T-cell cytotoxicity toward prostate cancer cells by specifically targeting T cells through CD3ε to prostate cancer cells expressing PSMA (prostate-specific membrane antigen). In vitro cross-linking of T cells with PSMA-expressing tumor cells by MOR209/ES414 triggered potent target-dependent tumor lysis and induction of target-dependent T-cell activation and proliferation. This activity occurred at low picomolar concentrations of MOR209/ES414 and was effective at low T-effector to tumor target cell ratios. In addition, cytotoxic activity was equivalent over a wide range of PSMA expression on target cells, suggesting that as few as 3,700 PSMA receptors per cell are sufficient for tumor lysis. In addition to high sensitivity and in vitro activity, MOR209/ES414 induced limited production of cytokines compared with other bispecific antibody formats. Pharmacokinetic analysis of MOR209/ES414 demonstrated a serum elimination half-life in NOD/SCID γ (NSG) mice of 4 days. Administration of MOR209/ES414 in murine xenograft models of human prostate cancer significantly inhibited tumor growth, prolonged survival, and decreased serum prostate-specific antigen levels only in the presence of adoptively transferred human T cells. On the basis of these preclinical findings, MOR209/ES414 warrants further investigation as a potential therapeutic for the treatment of CRPC. Mol Cancer Ther; 15(9); 2155-65. ©2016 AACR. PMID:27406985

  18. On the role of groES in the chaperonin-assisted folding reaction. Three case studies.

    PubMed

    Schmidt, M; Buchner, J; Todd, M J; Lorimer, G H; Viitanen, P V

    1994-04-01

    The mechanism by which correctly folded proteins are recovered from stable complexes with groEL is not well understood. Certain target proteins require ATP and groES, while others seemingly dispense with the cochaperonin. Here, we examine the chaperonin-assisted folding of ribulose-1,5-bisphosphate carboxylase, malate dehydrogenase, and citrate synthase, three proteins that are believed to require both chaperonin components for successful reactivation. Surprisingly, in all cases, the need for groES depended on the folding environment. Under "non-permissive" conditions, where unassisted spontaneous folding could not occur, reactivation to the native state required the complete chaperonin system (e.g. groEL, groES, and MgATP). However, under "permissive" conditions where spontaneous folding could occur groES was no longer mandatory. Instead, upon the addition of ATP alone, all three target proteins could be released from groEL, in a form that was capable of reaching the native state. In the permissive setting, groES merely accelerated the rate of the ATP-dependent release process. The results suggest that the incompletely folded protein species that are released from groEL, in the absence of groES, are not necessarily committed to the native state. Similar to the unassisted folding reaction, they still partition between productive and unproductive folding pathways in an environment-dependent manner. It follows that the mechanistic contribution of the co-chaperonin, groES, and its physiological significance in cellular protein folding, could be entirely missed in a permissive in vitro environment.

  19. THE RELATIONSHIP BETWEEN MOLECULAR GAS AND STAR FORMATION IN LOW-MASS E/S0 GALAXIES

    SciTech Connect

    Wei, Lisa H.; Vogel, Stuart N.; Kannappan, Sheila J.; Stark, David V.; Baker, Andrew J.; Laine, Seppo

    2010-12-10

    We consider the relationship between molecular gas and star formation surface densities in 19 morphologically defined E/S0s with stellar mass {approx}<4 x 10{sup 10} M{sub sun}, paying particular attention to those found on the blue sequence in color versus stellar mass parameter space, where spiral galaxies typically reside. While some blue-sequence E/S0s must be young major-merger remnants, many low-mass blue-sequence E/S0s appear much less disturbed and may be experiencing the milder starbursts associated with inner-disk building as spirals (re)grow. For a sample of eight E/S0s (four blue, two mid, and two red sequence) whose CARMA CO(1-0), Spitzer MIPS 24 {mu}m, and GALEX FUV emission distributions are spatially resolved on a 750 pc scale, we find roughly linear relationships between molecular gas and star formation surface densities within all galaxies, with power-law indices N = 0.6-1.9 (median 1.2). Adding 11 more blue-sequence E/S0s whose CO(1-0) emission is not as well resolved, we find that most of our E/S0s have global 1-8 kpc aperture-averaged molecular gas surface densities overlapping the range spanned by the disks and centers of spiral galaxies. While many of our E/S0s fall on the same Schmidt-Kennicutt relation as local spirals, {approx}80% (predominantly on the blue sequence) are offset toward apparently higher molecular gas star formation efficiency (i.e., shorter molecular gas depletion time). Possible interpretations of the elevated efficiencies include bursty star formation similar to that in local dwarf galaxies, H{sub 2} depletion in advanced starbursts, or simply a failure of the CO(1-0) emission to trace all of the molecular gas.

  20. GroEL/ES chaperonin modulates the mechanism and accelerates the rate of TIM-barrel domain folding.

    PubMed

    Georgescauld, Florian; Popova, Kristina; Gupta, Amit J; Bracher, Andreas; Engen, John R; Hayer-Hartl, Manajit; Hartl, F Ulrich

    2014-05-01

    The GroEL/ES chaperonin system functions as a protein folding cage. Many obligate substrates of GroEL share the (βα)8 TIM-barrel fold, but how the chaperonin promotes folding of these proteins is not known. Here, we analyzed the folding of DapA at peptide resolution using hydrogen/deuterium exchange and mass spectrometry. During spontaneous folding, all elements of the DapA TIM barrel acquire structure simultaneously in a process associated with a long search time. In contrast, GroEL/ES accelerates folding more than 30-fold by catalyzing segmental structure formation in the TIM barrel. Segmental structure formation is also observed during the fast spontaneous folding of a structural homolog of DapA from a bacterium that lacks GroEL/ES. Thus, chaperonin independence correlates with folding properties otherwise enforced by protein confinement in the GroEL/ES cage. We suggest that folding catalysis by GroEL/ES is required by a set of proteins to reach native state at a biologically relevant timescale, avoiding aggregation or degradation.

  1. Hanford Site Environment Safety and Health (ES and H) FY 1999 and FY 2000 Execution Commitment Summary

    SciTech Connect

    REEP, I.E.

    1999-12-01

    All sites in the U.S. Department of Energy (DOE) Complex prepare this report annually for the DOE Office of Environment, Safety and Health (EH). The purpose of this report is to provide a summary of the previous and current year's Environment, Safety and Health (ES&H) execution commitments and the S&H resources that support these activities. The fiscal year (FY) 1999 and 2000 information (Sieracki 1999) and data contained in the ''Hanford Site Environment, Safety and Health Fiscal Year 2001 Budget-Risk Management Summary'' (RL 1999) were the basis for preparing this report. Fiscal year 2000 finding of Office of Environmental Management (EM) and Office of Nuclear Energy, Science and Technology (NE) activities is based on the President's budget of $1,065.1 million and $28.0 million, plus $2.7 million carryover finding, respectively, as of October 31, 1999. Any funding changes as a result of the Congressional appropriation process will be reflected in the Fiscal Year 2002 ES&H Budget-Risk Management Summary to be issued in May 2000. This report provides the end-of-year status of FY 1999 ES&H execution commitments, including actual S&H expenditures, and describes planned FY 2000 ES&H execution commitments and the S&H resources needed to support those activities. This requirement is included in the ES&H ''Guidance for FY200l Budget Formulations and Execution'' (DOE 1999).

  2. Co-existence of intact stemness and priming of neural differentiation programs in mES cells lacking Trim71.

    PubMed

    Mitschka, Sibylle; Ulas, Thomas; Goller, Tobias; Schneider, Karin; Egert, Angela; Mertens, Jérôme; Brüstle, Oliver; Schorle, Hubert; Beyer, Marc; Klee, Kathrin; Xue, Jia; Günther, Patrick; Bassler, Kevin; Schultze, Joachim L; Kolanus, Waldemar

    2015-06-09

    Regulatory networks for differentiation and pluripotency in embryonic stem (ES) cells have long been suggested to be mutually exclusive. However, with the identification of many new components of these networks ranging from epigenetic, transcriptional, and translational to even post-translational mechanisms, the cellular states of pluripotency and early differentiation might not be strictly bi-modal, but differentiating stem cells appear to go through phases of simultaneous expression of stemness and differentiation genes. Translational regulators such as RNA binding proteins (RBPs) and micro RNAs (miRNAs) might be prime candidates for guiding a cell from pluripotency to differentiation. Using Trim71, one of two members of the Tripartite motif (Trim) protein family with RNA binding activity expressed in murine ES cells, we demonstrate that Trim71 is not involved in regulatory networks of pluripotency but regulates neural differentiation. Loss of Trim71 in mES cells leaves stemness and self-maintenance of these cells intact, but many genes required for neural development are up-regulated at the same time. Concordantly, Trim71(-/-) mES show increased neural marker expression following treatment with retinoic acid. Our findings strongly suggest that Trim71 keeps priming steps of differentiation in check, which do not pre-require a loss of the pluripotency network in ES cells.

  3. Co-existence of intact stemness and priming of neural differentiation programs in mES cells lacking Trim71

    PubMed Central

    Mitschka, Sibylle; Ulas, Thomas; Goller, Tobias; Schneider, Karin; Egert, Angela; Mertens, Jérôme; Brüstle, Oliver; Schorle, Hubert; Beyer, Marc; Klee, Kathrin; Xue, Jia; Günther, Patrick; Bassler, Kevin; Schultze, Joachim L.; Kolanus, Waldemar

    2015-01-01

    Regulatory networks for differentiation and pluripotency in embryonic stem (ES) cells have long been suggested to be mutually exclusive. However, with the identification of many new components of these networks ranging from epigenetic, transcriptional, and translational to even post-translational mechanisms, the cellular states of pluripotency and early differentiation might not be strictly bi-modal, but differentiating stem cells appear to go through phases of simultaneous expression of stemness and differentiation genes. Translational regulators such as RNA binding proteins (RBPs) and micro RNAs (miRNAs) might be prime candidates for guiding a cell from pluripotency to differentiation. Using Trim71, one of two members of the Tripartite motif (Trim) protein family with RNA binding activity expressed in murine ES cells, we demonstrate that Trim71 is not involved in regulatory networks of pluripotency but regulates neural differentiation. Loss of Trim71 in mES cells leaves stemness and self-maintenance of these cells intact, but many genes required for neural development are up-regulated at the same time. Concordantly, Trim71−/− mES show increased neural marker expression following treatment with retinoic acid. Our findings strongly suggest that Trim71 keeps priming steps of differentiation in check, which do not pre-require a loss of the pluripotency network in ES cells. PMID:26057209

  4. DISCOVERY OF VARIABILITY IN THE VERY HIGH ENERGY {gamma}-RAY EMISSION OF 1ES 1218+304 WITH VERITAS

    SciTech Connect

    Acciari, V. A.; Benbow, W.; Aliu, E.; Beilicke, M.; Bugaev, V.; Dickherber, R.; Boltuch, D.; Boettcher, M.; Bradbury, S. M.; Byrum, K.; Cesarini, A.; Ciupik, L.; Fortson, L.; Cogan, P.; Cui, W.; Finley, J. P.; Duke, C.; Falcone, A.; Finnegan, G.; Furniss, A.

    2010-02-01

    We present results from an intensive VERITAS monitoring campaign of the high-frequency peaked BL Lac object 1ES 1218+304 in 2008/2009. Although 1ES 1218+304 was detected previously by MAGIC and VERITAS at a persistent level of {approx}6% of the Crab Nebula flux, the new VERITAS data reveal a prominent flare reaching {approx}20% of the Crab. While very high energy (VHE) flares are quite common in many nearby blazars, the case of 1ES 1218+304 (redshift z = 0.182) is particularly interesting since it belongs to a group of blazars that exhibit unusually hard VHE spectra considering their redshifts. When correcting the measured spectra for absorption by the extragalactic background light, 1ES 1218+304 and a number of other blazars are found to have differential photon indices {gamma}{<=} 1.5. The difficulty in modeling these hard spectral energy distributions in blazar jets has led to a range of theoretical {gamma}-ray emission scenarios, one of which is strongly constrained by these new VERITAS observations. We consider the implications of the observed light curve of 1ES 1218+304, which shows day scale flux variations, for shock acceleration scenarios in relativistic jets, and in particular for the viability of kiloparsec-scale jet emission scenarios.

  5. Solar cycle dependent characteristics of the equatorial blanketing Es layers and associated irregularities

    NASA Astrophysics Data System (ADS)

    Devasia, C. V.; Sreeja, V.; Ravindran, S.

    2006-11-01

    The occurrence of blanketing type Es (Esb) layers and associated E-region irregularities over the magnetic equatorial location of Trivandrum (8.5° N; 77° E; dip ~0.5°) during the summer solstitial months of May, June, July and August has been investigated in detail for the period 1986-2000 to bring out the variabilities in their characteristics with the solar cycle changes. The study has been made using the ionosonde and magnetometer data of Trivandrum from 1986-2000 along with the available data from the 54.95 MHz VHF backscatter radar at Trivandrum for the period 1995-2000. The appearance of blanketing Es layers during these months is observed to be mostly in association with the occurrence of afternoon Counter Electrojet (CEJ) events. The physical process leading to the occurrence of a CEJ event is mainly controlled by the nature of the prevailing electro dynamical/neutral dynamical conditions before the event. Hence it is natural that the Esb layer characteristics like the frequency of occurrence, onset time, intensity, nature of gradients in its top and bottom sides etc are also affected by the nature of the background electro dynamical /neutral dynamical processes which in turn are strongly controlled by the solar activity changes. The occurrence of Esb layers during the solstitial months is found to show very strong solar activity dependence with the occurrence frequency being very large during the solar minimum years and very low during solar maximum years. The intensity of the VHF radar backscattered signals from the Esb irregularities is observed to be controlled by the relative roles of the direction and magnitude of the prevailing vertical polarization electric field and the vertical electron density gradient of the prevailing Esb layer depending on the phase of the solar cycle. The gradient of the Esb layer shows a more dominant role in the generation of gradient instabilities during solar minimum periods while it is the electric field that has a

  6. A Single-Ring Mitochondrial Chaperonin (Hsp60-Hsp10) Can Substitute for GroEL-GroES In Vivo

    PubMed Central

    Nielsen, Kåre L.; McLennan, Neil; Masters, Millicent; Cowan, Nicholas J.

    1999-01-01

    Chaperonins participate in the facilitated folding of a variety of proteins in vivo. To see whether the same spectrum of target proteins can be productively folded by the double-ring prokaryotic chaperonin GroEL-GroES and its single-ring human mitochondrial homolog, Hsp60-Hsp10, we expressed the latter in an Escherichia coli strain engineered so that the groE operon is under strict regulatory control. We found that expression of Hsp60-Hsp10 restores viability to cells that no longer express GroEL-GroES, formally demonstrating that Hsp60-Hsp10 can carry out all essential in vivo functions of GroEL-GroES. PMID:10482535

  7. Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES

    PubMed Central

    Chi, Haixia; Wang, Xiaoqiang; Li, Jiqiang; Ren, Hao; Huang, Fang

    2015-01-01

    The in vitro folding of newly translated human CC chemokine receptor type 5 (CCR5), which belongs to the physiologically important family of G protein-coupled receptors (GPCRs), has been studied in a cell-free system supplemented with the surfactant Brij-35. The freshly synthesized CCR5 can spontaneously fold into its biologically active state but only slowly and inefficiently. However, on addition of the GroEL-GroES molecular chaperone system, the folding of the nascent CCR5 was significantly enhanced, as was the structural stability and functional expression of the soluble form of CCR5. The chaperonin GroEL was partially effective on its own, but for maximum efficiency both the GroEL and its GroES lid were necessary. These results are direct evidence for chaperone-assisted membrane protein folding and therefore demonstrate that GroEL-GroES may be implicated in the folding of membrane proteins. PMID:26585937

  8. The sequence of the groES and groEL genes from the mouse pneumonitis agent of Chlamydia trachomatis.

    PubMed

    Ho, Y; Zhang, Y X

    1994-04-01

    The co-transcribed structural genes, groES and groEL, of the groE stress response operon from Chlamydia trachomatis mouse pneumonitis (MoPn), were cloned and sequenced. The calculated molecular masses of the encoded heat-shock proteins (Hsp), a small Hsp (GroES) and Hsp60 (GroEL), are 11,089 and 58,367 Da, respectively. By comparison with other known chlamydial groES and groEL sequences, there is 89 and 94% nucleotide (nt) identity with C. trachomatis human strains (serovars A and L2), 77 and 82% with C. psittaci strain GPIC, and 75 and 80% with C. pneumoniae isolate AR-39. At the amino-acid level, the MoPn Hsp60 shows a 99% identity with those from C. trachomatis human strains. In a mouse model, MoPn Hsp60 could prove useful in deciphering the pathogenesis of human chlamydial diseases.

  9. Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES

    NASA Astrophysics Data System (ADS)

    Chi, Haixia; Wang, Xiaoqiang; Li, Jiqiang; Ren, Hao; Huang, Fang

    2015-11-01

    The in vitro folding of newly translated human CC chemokine receptor type 5 (CCR5), which belongs to the physiologically important family of G protein-coupled receptors (GPCRs), has been studied in a cell-free system supplemented with the surfactant Brij-35. The freshly synthesized CCR5 can spontaneously fold into its biologically active state but only slowly and inefficiently. However, on addition of the GroEL-GroES molecular chaperone system, the folding of the nascent CCR5 was significantly enhanced, as was the structural stability and functional expression of the soluble form of CCR5. The chaperonin GroEL was partially effective on its own, but for maximum efficiency both the GroEL and its GroES lid were necessary. These results are direct evidence for chaperone-assisted membrane protein folding and therefore demonstrate that GroEL-GroES may be implicated in the folding of membrane proteins.

  10. Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES.

    PubMed

    Chi, Haixia; Wang, Xiaoqiang; Li, Jiqiang; Ren, Hao; Huang, Fang

    2015-11-20

    The in vitro folding of newly translated human CC chemokine receptor type 5 (CCR5), which belongs to the physiologically important family of G protein-coupled receptors (GPCRs), has been studied in a cell-free system supplemented with the surfactant Brij-35. The freshly synthesized CCR5 can spontaneously fold into its biologically active state but only slowly and inefficiently. However, on addition of the GroEL-GroES molecular chaperone system, the folding of the nascent CCR5 was significantly enhanced, as was the structural stability and functional expression of the soluble form of CCR5. The chaperonin GroEL was partially effective on its own, but for maximum efficiency both the GroEL and its GroES lid were necessary. These results are direct evidence for chaperone-assisted membrane protein folding and therefore demonstrate that GroEL-GroES may be implicated in the folding of membrane proteins.

  11. Symmetric GroEL:GroES2 complexes are the protein-folding functional form of the chaperonin nanomachine

    PubMed Central

    Yang, Dong; Ye, Xiang; Lorimer, George H.

    2013-01-01

    Using calibrated FRET, we show that the simultaneous occupancy of both rings of GroEL by ATP and GroES occurs, leading to the rapid formation of symmetric GroEL:GroES2 “football” particles regardless of the presence or absence of substrate protein (SP). In the absence of SP, these symmetric particles revert to asymmetric GroEL:GroES1 “bullet” particles. The breakage of GroES symmetry requires the stochastic hydrolysis of ATP and the breakage of nucleotide symmetry. These asymmetric particles are both persistent and dynamic; they turnover via the asymmetric cycle. When challenged with SP, however, they revert to symmetric particles within a second. In the presence of SP, the symmetric particles are also persistent and dynamic. They turn over via the symmetric cycle. Under these conditions, the stochastic hydrolysis of ATP and the breakage of nucleotide symmetry also occur within the ensemble of particles. However, on account of SP-catalyzed ADP/ATP exchange, GroES symmetry is rapidly restored. The residence time of both GroES and SP on functional GroEL is reduced to ∼1 s, enabling many more iterations than was previously believed possible, consistent with the iterative annealing mechanism. This result is inconsistent with currently accepted models. Using a foldable SP, we show that as the SP folds to the native state and the population of unfolded SP declines, the population of symmetric particles reverts to asymmetric particles in parallel, a result that is consistent with the former being the folding functional form. PMID:24167279

  12. Algunas mujeres con cáncer de seno pueden abstenerse de quimioterapia

    Cancer.gov

    Resumen de resultados del estudio TAILORx indica que mujeres con cáncer de seno receptor de hormonas en estadio inicial tienen un riesgo bajo de recurrencia según una prueba de expresión de 21 genes.

  13. An ES-Like Pluripotent State in FGF-Dependent Murine iPS cells

    PubMed Central

    Ungaro, Federica; Prigione, Alessandro; Chen, Hsu-Hsin; Welling, Maaike; Eijpe, Maureen; Mostoslavsky, Gustavo; Tesar, Paul; Adjaye, James; Geijsen, Niels; Broccoli, Vania

    2010-01-01

    Recent data demonstrates that stem cells can exist in two morphologically, molecularly and functionally distinct pluripotent states; a naïve LIF-dependent pluripotent state which is represented by murine embryonic stem cells (mESCs) and an FGF-dependent primed pluripotent state represented by murine and rat epiblast stem cells (EpiSCs). We find that derivation of induced pluripotent stem cells (iPSCs) under EpiSC culture conditions yields FGF-dependent iPSCs from hereon called FGF-iPSCs) which, unexpectedly, display naïve ES-like/ICM properties. FGF-iPSCs display X-chromosome activation, multi-lineage differentiation, teratoma competence and chimera contribution in vivo. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions. Characterization of the key molecular signalling pathways revealed FGF-iPSCs to depend on the Activin/Nodal and FGF pathways, while signalling through the JAK-STAT pathway is not required for FGF-iPS cell maintenance. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions. PMID:21209851

  14. Multiwavelength Observations and Modeling of 1ES 1959+650 in a Low Flux State

    NASA Astrophysics Data System (ADS)

    Aliu, E.; Archambault, S.; Arlen, T.; Aune, T.; Beilicke, M.; Benbow, W.; Bird, R.; Böttcher, M.; Bouvier, A.; Bugaev, V.; Byrum, K.; Cesarini, A.; Ciupik, L.; Collins-Hughes, E.; Connolly, M. P.; Cui, W.; Dickherber, R.; Duke, C.; Dumm, J.; Errando, M.; Falcone, A.; Federici, S.; Feng, Q.; Finley, J. P.; Finnegan, G.; Fortson, L.; Furniss, A.; Galante, N.; Gall, D.; Gillanders, G. H.; Griffin, S.; Grube, J.; Gyuk, G.; Hanna, D.; Holder, J.; Hughes, G.; Humensky, T. B.; Kaaret, P.; Kertzman, M.; Khassen, Y.; Kieda, D.; Krawczynski, H.; Krennrich, F.; Lang, M. J.; Madhavan, A. S.; Maier, G.; Majumdar, P.; McArthur, S.; McCann, A.; Moriarty, P.; Mukherjee, R.; Nelson, T.; O'Faoláin de Bhróithe, A.; Ong, R. A.; Orr, M.; Otte, A. N.; Park, N.; Perkins, J. S.; Pichel, A.; Pohl, M.; Popkow, A.; Prokoph, H.; Quinn, J.; Ragan, K.; Reyes, L. C.; Reynolds, P. T.; Roache, E.; Saxon, D. B.; Schroedter, M.; Sembroski, G. H.; Skole, C.; Smith, A. W.; Staszak, D.; Telezhinsky, I.; Theiling, M.; Tyler, J.; Varlotta, A.; Vassiliev, V. V.; Wakely, S. P.; Weekes, T. C.; Weinstein, A.; Welsing, R.; Williams, D. A.; Zitzer, B.

    2013-09-01

    We report on the VERITAS observations of the high-frequency peaked BL Lac object 1ES 1959+650 in the period 2007-2011. This source is detected at TeV energies by VERITAS at 16.4 standard deviation (σ) significance in 7.6 hr of observation in a low flux state. A multiwavelength spectral energy distribution (SED) is constructed from contemporaneous data from VERITAS, Fermi-LAT, RXTE PCA, and Swift UVOT. Swift XRT data is not included in the SED due to a lack of simultaneous observations with VERITAS. In contrast to the orphan γ-ray flare exhibited by this source in 2002, the X-ray flux of the source is found to vary by an order of magnitude, while other energy regimes exhibit less variable emission. A quasi-equilibrium synchrotron self-Compton model with an additional external radiation field is used to describe three SEDs corresponding to the lowest, highest, and average X-ray states. The variation in the X-ray spectrum is modeled by changing the electron injection spectral index, with minor adjustments of the kinetic luminosity in electrons. This scenario produces small-scale flux variability of the order of <~ 2 in the high energy (E > 1 MeV) and very high energy (E > 100 GeV) γ-ray regimes, which is corroborated by the Fermi-LAT, VERITAS, and Whipple 10 m telescope light curves.

  15. CHANG-ES. VII. Magnetic Outflows from the Virgo Cluster Galaxy NGC 4388

    NASA Astrophysics Data System (ADS)

    Damas-Segovia, A.; Beck, R.; Vollmer, B.; Wiegert, T.; Krause, M.; Irwin, J.; Weżgowiec, M.; Li, J.; Dettmar, R.-J.; English, J.; Wang, Q. D.

    2016-06-01

    We investigate the effects of ram pressure on the ordered magnetic field of a galaxy hosting a radio halo and strong nuclear outflows. New radio images in total and polarized intensity of the edge-on Virgo galaxy NGC 4388 were obtained within the CHANG-ES EVLA project. The unprecedented noise level reached allows us to detect striking new features of the ordered magnetic field. The nuclear outflow extends far into the halo to about 5 kpc from the center and is spatially correlated with the {{H}}α and X-ray emission. For the first time, the southern outflow is detected. Above and below both spiral arms we find extended blobs of polarized emission with an ordered field oriented perpendicular to the disk. The synchrotron lifetime of the cosmic-ray electrons (CREs) in these regions yields a mean outflow velocity of 270+/- 70 {km} {{{s}}}-1, in agreement with a galactic wind scenario. The observed symmetry of the polarized halo features in NGC 4388 excludes a compression of the halo gas by the ram pressure of the intracluster medium (ICM). The assumption of equilibrium between the halo pressure and the ICM ram pressure yields an estimate of the ICM density that is consistent with both the ICM density derived from X-ray observations and the recent Planck Sunyaev-Zel’dovich measurements. The detection of a faint radio halo around cluster galaxies could thus be used for an estimate of ICM ram pressure.

  16. The Herschel view of HII regions in M 33 (HERM33ES)

    NASA Astrophysics Data System (ADS)

    Verley, S.; Relaño, M.; Kramer, C.; Xilouris, E. M.; Boquien, M.; Calzetti, D.; Combes, F.; Buchbender, C.; Braine, J.; Quintana-Lacaci, G.; Tabatabaei, F. S.; Lord, S.; Israel, F.; Stacey, G.; van der Werf, P.

    2010-12-01

    Within the framework of the HERM33ES Key Project (Kramer et al. 2010), using the high resolution and sensitivity of the Herschel photometric data, we study the compact emission in the Local Group spiral galaxy M 33. We present a catalogue of 159 compact emission sources in M 33 identified by SExtractor in the 250 μm SPIRE band which is the one that provides the best spatial resolution. We measure fluxes at 24 μm and Hα for those 159 extracted sources. We find a very strong Pearson correlation coefficient with the MIPS 24 μm emission (r24 = 0.94) and a rather strong correlation with the Hα emission, although with more scatter (rHα = 0.83). Due to the very strong link between the 250 μm compact emission and the 24 μm and Hα emissions, by recovering the star formation rate from standard recipes for H II regions, we are able to provide star formation rate calibrations based on the 250 μm compact emission alone. Finally, the morphological study of a set of three Hα shells shows that there is a displacement between far-ultraviolet and the SPIRE bands, while the Hα structure is in general much more coincident with the cool dust.

  17. 2D/3D registration with the CMA-ES method

    NASA Astrophysics Data System (ADS)

    Gong, Ren Hui; Abolmaesumi, Purang

    2008-03-01

    In this paper, we propose a new method for 2D/3D registration and report its experimental results. The method employs the Covariance Matrix Adaptation Evolution Strategy (CMA-ES) algorithm to search for an optimal transformation that aligns the 2D and 3D data. The similarity calculation is based on Digitally Reconstructed Radiographs (DRRs), which are dynamically generated from the 3D data using a hardware-accelerated technique - Adaptive Slice Geometry Texture Mapping (ASGTM). Three bone phantoms of different sizes and shapes were used to test our method: a long femur, a large pelvis, and a small scaphoid. A collection of experiments were performed to register CT to fluoroscope and DRRs of these phantoms using the proposed method and two prior work, i.e. our previously proposed Unscented Kalman Filter (UKF) based method and a commonly used simplex-based method. The experimental results showed that: 1) with slightly more computation overhead, the proposed method was significantly more robust to local minima than the simplex-based method; 2) while as robust as the UKF-based method in terms of capture range, the new method was not sensitive to the initial values of its exposed control parameters, and has also no special requirement about the cost function; 3) the proposed method was fast and consistently achieved the best accuracies in all compared methods.

  18. Evaluating the genomic and sequence integrity of human ES cell lines; comparison to normal genomes.

    PubMed

    Funk, Walter D; Labat, Ivan; Sampathkumar, Janani; Gourraud, Pierre-Antoine; Oksenberg, Jorge R; Rosler, Elen; Steiger, Daniel; Sheibani, Nadia; Caillier, Stacy; Stache-Crain, Birgit; Johnson, Julie A; Meisner, Lorraine; Lacher, Markus D; Chapman, Karen B; Park, Myung Jin; Shin, Kyoung-Jin; Drmanac, Rade; West, Michael D

    2012-03-01

    Copy number variation (CNV) is a common chromosomal alteration that can occur during in vitro cultivation of human cells and can be accompanied by the accumulation of mutations in coding region sequences. We describe here a systematic application of current molecular technologies to provide a detailed understanding of genomic and sequence profiles of human embryonic stem cell (hESC) lines that were derived under GMP-compliant conditions. We first examined the overall chromosomal integrity using cytogenetic techniques to determine chromosome count, and to detect the presence of cytogenetically aberrant cells in the culture (mosaicism). Assays of copy number variation, using both microarray and sequence-based analyses, provide a detailed view genomic variation in these lines and shows that in early passage cultures of these lines, the size range and distribution of CNVs are entirely consistent with those seen in the genomes of normal individuals. Similarly, genome sequencing shows variation within these lines that is completely within the range seen in normal genomes. Important gene classes, such as tumor suppressors and genetic disease genes, do not display overtly disruptive mutations that could affect the overall safety of cell-based therapeutics. Complete sequence also allows the analysis of important transplantation antigens, such as ABO and HLA types. The combined application of cytogenetic and molecular technologies provides a detailed understanding of genomic and sequence profiles of GMP produced ES lines for potential use as therapeutic agents. PMID:22265736

  19. Prevalence of helmet use among motorcycle users in Dar Es Salaam, Tanzania

    PubMed Central

    Kauky, Cosmas George; Kishimba, Rogath Saika; Urio, Loveness John; Abade, Ahmed Mohammed; Mghamba, Janneth Maridadi

    2015-01-01

    Introduction The purpose of this study was to determine prevalence of helmet use among motorcyclists as one of the preventive measures for road traffic injuries. Methods A cross sectional observational survey was conducted in the 3 Districts (Kinondoni, Ilala and Temeke) that make Dar es Salaam. Tanzania. A standardized line-listing form and checklist were used to record the drivers and passengers use of helmet as observed by study investigators. Data for helmet use was collected on one weekday and one weekend day. Time for observation was during the rush hour in the morning, noon and evening. Then data were entered into Epi Info 3.5.1 analysis Results A total of 7,678 motorcycle drivers and 4,328 passengers observed in this study. Drivers were almost male (98.8%) and 73.2% of all passengers were males. The prevalence use of helmet use among motorcyclist's riders was 82.1% and among passengers was 22.5%. Proportion of helmet use in drivers and passengers observed were relatively similar during weekday and weekend day and time of observation. Conclusion This study showed the relative high helmet use among motorcyclist riders though very low in passengers. This study recommends increased community awareness on helmet use among passengers and enforcement and revival of road safety laws of passengers and motorcyclists on helmet use. PMID:26309470

  20. The therapeutic potential of the filarial nematode-derived immunodulator, ES-62 in inflammatory disease.

    PubMed

    Harnett, M M; Melendez, A J; Harnett, W

    2010-03-01

    The dramatic recent rise in the incidence of allergic or autoimmune inflammatory diseases in the West has been proposed to reflect the lack of appropriate priming of the immune response by infectious agents such as parasitic worms during childhood. Consistent with this, there is increasing evidence supporting an inverse relationship between worm infection and T helper type 1/17 (Th1/17)-based inflammatory disorders such as rheumatoid arthritis, inflammatory bowel disease, type 1 diabetes and multiple sclerosis. Perhaps more surprisingly, given that such worms often induce strong Th2-type immune responses, there also appears to be an inverse correlation between parasite load and atopy. These findings therefore suggest that the co-evolution of helminths with hosts, which has resulted in the ability of worms to modulate inflammatory responses to promote parasite survival, has also produced the benefit of protecting the host from pathological lesions arising from aggressive proinflammatory responses to infection or, indeed, aberrant inflammatory responses underlying autoimmune and allergic disorders. By focusing upon the properties of the filarial nematode-derived immunomodulatory molecule, ES-62, in this review we shall discuss the potential of exploiting the immunomodulatory products of parasitic worms to identify and develop novel therapeutics for inflammation. PMID:19968663

  1. Heredity, development and evolution: the unmodern synthesis of E.S. Russell.

    PubMed

    Esposito, Maurizio

    2013-09-01

    In 1930, while R.A. Fisher, J.B.S. Haldane, E.B. Ford and S.G. Wright were laying the foundations of what a decade later J.S. Huxley dubbed "Modern Synthesis", E.S. Russell published a groundbreaking work, The Interpretation of Development and Heredity. In this book Russell not only condemned Mendelian genetics and neo-Darwinism, but also proposed an alternative synthesis unifying heredity, development, and evolution. The book did not represent the work of a mind operating in isolation. Rather, it was a synthetic work connecting ideas and doctrines of many influential scientists working in Europe and the USA. Through the analysis of archival documents and rarely or never mentioned sources, this article provides an unconventional picture of Russell's theoretical biology. It will be shown that Russell was an international celebrity; he was at the centre of a large network of scholars who shared his ideas and insights. He was one of several biologists arguing for a different synthesis; a synthesis perhaps less visible, less institutionalised, and less 'modern', nevertheless with its influential advocates and international support. Finally, this study shows that Russell's synthesis was not rooted in the classic pantheon of towering figures in the history of biology, i.e. Darwin, Wallace, and Mendel, but was based on the teachings of Kant, Goethe, Cuvier, von Baer, and Müller. PMID:23408008

  2. Pit latrine emptying behavior and demand for sanitation services in Dar Es Salaam, Tanzania.

    PubMed

    Jenkins, Marion W; Cumming, Oliver; Cairncross, Sandy

    2015-02-27

    Pit latrines are the main form of sanitation in unplanned areas in many rapidly growing developing cities. Understanding demand for pit latrine fecal sludge management (FSM) services in these communities is important for designing demand-responsive sanitation services and policies to improve public health. We examine latrine emptying knowledge, attitudes, behavior, trends and rates of safe/unsafe emptying, and measure demand for a new hygienic latrine emptying service in unplanned communities in Dar Es Salaam (Dar), Tanzania, using data from a cross-sectional survey at 662 residential properties in 35 unplanned sub-wards across Dar, where 97% had pit latrines. A picture emerges of expensive and poor FSM service options for latrine owners, resulting in widespread fecal sludge exposure that is likely to increase unless addressed. Households delay emptying as long as possible, use full pits beyond what is safe, face high costs even for unhygienic emptying, and resort to unsafe practices like 'flooding out'. We measured strong interest in and willingness to pay (WTP) for the new pit emptying service at 96% of residences; 57% were WTP≥U.S. $17 to remove ≥200 L of sludge. Emerging policy recommendations for safe FSM in unplanned urban communities in Dar and elsewhere are discussed.

  3. THE BROADBAND INFRARED EMISSION SPECTRUM OF THE EXOPLANET TrES-3

    SciTech Connect

    Fressin, Francois; Knutson, Heather A.; Charbonneau, David; O'Donovan, Francis T.; Burrows, Adam; Spiegel, David; Deming, Drake; Mandushev, Georgi

    2010-03-01

    We use the Spitzer Space Telescope to estimate the dayside thermal emission of the exoplanet TrES-3 integrated in the 3.6, 4.5, 5.8, and 8.0 {mu}m bandpasses of the Infrared Array Camera (IRAC) instrument. We observe two secondary eclipses and find relative eclipse depths of 0.00346 +- 0.00035, 0.00372 +- 0.00054, 0.00449 +- 0.00097, and 0.00475 +- 0.00046, respectively, in the four IRAC bandpasses. We combine our results with the earlier K-band measurement of De Mooij et al., and compare them with models of the planetary emission. We find that the planet does not require the presence of an inversion layer in the high atmosphere. This is the first very strongly irradiated planet that does not have a temperature inversion, which indicates that stellar or planetary characteristics other than temperature have an important impact on temperature inversion. De Mooij and Snellen also detected a possible slight offset in the timing of the secondary eclipse in the K band. However, based on our four Spitzer channels, we place a 3sigma upper limit of |ecos(omega)| < 0.0056, where e is the planet's orbital eccentricity and omega is the longitude of the periastron. This result strongly indicates that the orbit is circular, as expected from tidal circularization theory.

  4. Pit Latrine Emptying Behavior and Demand for Sanitation Services in Dar Es Salaam, Tanzania

    PubMed Central

    Jenkins, Marion W.; Cumming, Oliver; Cairncross, Sandy

    2015-01-01

    Pit latrines are the main form of sanitation in unplanned areas in many rapidly growing developing cities. Understanding demand for pit latrine fecal sludge management (FSM) services in these communities is important for designing demand-responsive sanitation services and policies to improve public health. We examine latrine emptying knowledge, attitudes, behavior, trends and rates of safe/unsafe emptying, and measure demand for a new hygienic latrine emptying service in unplanned communities in Dar Es Salaam (Dar), Tanzania, using data from a cross-sectional survey at 662 residential properties in 35 unplanned sub-wards across Dar, where 97% had pit latrines. A picture emerges of expensive and poor FSM service options for latrine owners, resulting in widespread fecal sludge exposure that is likely to increase unless addressed. Households delay emptying as long as possible, use full pits beyond what is safe, face high costs even for unhygienic emptying, and resort to unsafe practices like ‘flooding out’. We measured strong interest in and willingness to pay (WTP) for the new pit emptying service at 96% of residences; 57% were WTP ≥U.S. $17 to remove ≥200 L of sludge. Emerging policy recommendations for safe FSM in unplanned urban communities in Dar and elsewhere are discussed. PMID:25734790

  5. The health-related microbiological quality of bottled drinking water sold in Dar es Salaam, Tanzania.

    PubMed

    Kassenga, Gabriel R

    2007-03-01

    The consumption of bottled and plastic-bagged drinking water in Tanzania has increased largely because of the deteriorating quality of tap water. It is uncertain whether these water products are safe for drinking. In this study, the microbiological quality of bottled and plastic-bagged drinking water sold in Dar es Salaam, Tanzania, was investigated. One hundred and thirty samples representing 13 brands of bottled water collected from shops, supermarkets and street vendors were analysed for total coliform and faecal coliform organisms as well as heterotrophic bacteria. These were compared with 61 samples of tap water. Heterotrophic bacteria were detected in 92% of the bottled water samples analysed. Total and faecal coliform bacteria were present in 4.6% and 3.6%, respectively, of samples analysed with a tendency for higher contamination rates in plastic-bagged drinking water. Microbiological quality of tap water was found to be worse compared with bottled water, with 49.2% and 26.2% of sampling points showing the presence of total coliform and faecal coliform organisms, respectively. The results suggest caution and vigilance to avert outbreaks of waterborne diseases from these types of drinking water.

  6. Heredity, development and evolution: the unmodern synthesis of E.S. Russell.

    PubMed

    Esposito, Maurizio

    2013-09-01

    In 1930, while R.A. Fisher, J.B.S. Haldane, E.B. Ford and S.G. Wright were laying the foundations of what a decade later J.S. Huxley dubbed "Modern Synthesis", E.S. Russell published a groundbreaking work, The Interpretation of Development and Heredity. In this book Russell not only condemned Mendelian genetics and neo-Darwinism, but also proposed an alternative synthesis unifying heredity, development, and evolution. The book did not represent the work of a mind operating in isolation. Rather, it was a synthetic work connecting ideas and doctrines of many influential scientists working in Europe and the USA. Through the analysis of archival documents and rarely or never mentioned sources, this article provides an unconventional picture of Russell's theoretical biology. It will be shown that Russell was an international celebrity; he was at the centre of a large network of scholars who shared his ideas and insights. He was one of several biologists arguing for a different synthesis; a synthesis perhaps less visible, less institutionalised, and less 'modern', nevertheless with its influential advocates and international support. Finally, this study shows that Russell's synthesis was not rooted in the classic pantheon of towering figures in the history of biology, i.e. Darwin, Wallace, and Mendel, but was based on the teachings of Kant, Goethe, Cuvier, von Baer, and Müller.

  7. Retroviral Infection of hES Cells Produces Random-like Integration Patterns

    PubMed Central

    Lim, Kwang-il

    2012-01-01

    Retroviral integration provides us with a powerful tool to realize prolonged gene expressions that are often critical to gene therapy. However, the perturbation of gene regulations in host cells by viral genome integration can lead to detrimental effects, yielding cancer. The oncogenic potential of retroviruses is linked to the preference of retroviruses to integrate into genomic regions that are enriched in gene regulatory elements. To better navigate the double-edged sword of retroviral integration we need to understand how retroviruses select their favored genomic loci during infections. In this study I showed that in addition to host proteins that tether retroviral pre-integration complexes to specific genomic regions, the epigenetic architecture of host genome might strongly affect retroviral integration patterns. Specifically, retroviruses showed their characteristic integration preference in differentiated somatic cells. In contrast, retroviral infections of hES cells, which are known to display decondensed chromatin, produced random-like integration patterns lacking of strong preference for regulatory-element-rich genomic regions. Better identification of the cellular and viral factors that determine retroviral integration patterns will facilitate the design of retroviral vectors for safer use in gene therapy. PMID:22526396

  8. Medicalization and morality in a weak state: health, hygiene and water in Dar Es Salaam, Tanzania.

    PubMed

    Obrist, Brigit

    2004-04-01

    Inspired by Foucault, many studies have examined the medicalization of everyday life in Western societies. This paper reconsiders potentials and limitations of this concept in an African city. Grounded in ethnographic research in Dar es Salaam, Tanzania, it concentrates on cleanliness, health and water in a lower middle-class neighbourhood. The findings show that women are familiar with professional health development discourses emphasizing cleanliness as a high value linked to bodily and domestic health. These discourses have been diffused in schools, clinics and other institutions during the colonial and socialist period. Women not only refer to these discourses, they try to reproduce them in daily practice and even demand them. This coercive yet voluntary nature of institutionalized discourses points to 'paradoxes of medicalization' also found in Western societies. It acquires, however, different meanings in a weak state like contemporary Tanzania which hardly manages to institutionalize medicalization through professional practice. Under such conditions, women who choose to follow health development discourses suffer a heavier practical, intellectual and emotional burden than those who are less committed. This may at least partly explain why many women assume a pragmatic stance towards the medicalization of everyday life.

  9. Sox2 regulatory region 2 sequence works as a DNA nuclear targeting sequence enhancing the efficiency of an exogenous gene expression in ES cells

    SciTech Connect

    Funabashi, Hisakage; Takatsu, Makoto; Saito, Mikako; Matsuoka, Hideaki

    2010-10-01

    Research highlights: {yields} SV40-DTS worked as a DTS in ES cells as well as other types of cells. {yields} Sox2 regulatory region 2 worked as a DTS in ES cells and thus was termed as SRR2-DTS. {yields} SRR2-DTS was suggested as an ES cell-specific DTS. -- Abstract: In this report, the effects of two DNA nuclear targeting sequence (DTS) candidates on the gene expression efficiency in ES cells were investigated. Reporter plasmids containing the simian virus 40 (SV40) promoter/enhancer sequence (SV40-DTS), a DTS for various types of cells but not being reported yet for ES cells, and the 81 base pairs of Sox2 regulatory region 2 (SRR2) where two transcriptional factors in ES cells, Oct3/4 and Sox2, are bound (SRR2-DTS), were introduced into cytoplasm in living cells by femtoinjection. The gene expression efficiencies of each plasmid in mouse insulinoma cell line MIN6 cells and mouse ES cells were then evaluated. Plasmids including SV40-DTS and SRR2-DTS exhibited higher gene expression efficiency comparing to plasmids without these DTSs, and thus it was concluded that both sequences work as a DTS in ES cells. In addition, it was suggested that SRR2-DTS works as an ES cell-specific DTS. To the best of our knowledge, this is the first report to confirm the function of DTSs in ES cells.

  10. Investigating Broadband Variability of the TeV Blazar 1ES 1959+650

    NASA Astrophysics Data System (ADS)

    Aliu, E.; Archambault, S.; Arlen, T.; Aune, T.; Barnacka, A.; Beilicke, M.; Benbow, W.; Berger, K.; Bird, R.; Bouvier, A.; Buckley, J. H.; Bugaev, V.; Cerruti, M.; Chen, X.; Ciupik, L.; Collins-Hughes, E.; Connolly, M. P.; Cui, W.; Dumm, J.; Eisch, J. D.; Falcone, A.; Federici, S.; Feng, Q.; Finley, J. P.; Fleischhack, H.; Fortin, P.; Fortson, L.; Furniss, A.; Galante, N.; Gillanders, G. H.; Griffin, S.; Griffiths, S. T.; Grube, J.; Gyuk, G.; Håkansson, N.; Hanna, D.; Holder, J.; Hughes, G.; Hughes, Z.; Humensky, T. B.; Johnson, C. A.; Kaaret, P.; Kar, P.; Kertzman, M.; Khassen, Y.; Kieda, D.; Krawczynski, H.; Krennrich, F.; Lang, M. J.; Madhavan, A. S.; Majumdar, P.; McArthur, S.; McCann, A.; Meagher, K.; Millis, J.; Moriarty, P.; Mukherjee, R.; Nelson, T.; Nieto, D.; O'Faoláin de Bhróithe, A.; Ong, R. A.; Otte, A. N.; Park, N.; Perkins, J. S.; Pohl, M.; Popkow, A.; Prokoph, H.; Quinn, J.; Ragan, K.; Rajotte, J.; Reyes, L. C.; Reynolds, P. T.; Richards, G. T.; Roache, E.; Sadun, A.; Santander, M.; Sembroski, G. H.; Shahinyan, K.; Sheidaei, F.; Smith, A. W.; Staszak, D.; Telezhinsky, I.; Theiling, M.; Tyler, J.; Varlotta, A.; Vassiliev, V. V.; Vincent, S.; Wakely, S. P.; Weekes, T. C.; Weinstein, A.; Welsing, R.; Wilhelm, A.; Williams, D. A.; Zitzer, B.; VERITAS Collaboration; Böttcher, M.; Fumagalli, M.

    2014-12-01

    We summarize broadband observations of the TeV-emitting blazar 1ES 1959+650, including optical R-band observations by the robotic telescopes Super-LOTIS and iTelescope, UV observations by Swift Ultraviolet and Optical Telescope, X-ray observations by the Swift X-ray Telescope, high-energy gamma-ray observations with the Fermi Large Area Telescope, and very-high-energy (VHE) gamma-ray observations by VERITAS above 315 GeV, all taken between 2012 April 17 and 2012 June 1 (MJD 56034 and 56079). The contemporaneous variability of the broadband spectral energy distribution is explored in the context of a simple synchrotron self Compton (SSC) model. In the SSC emission scenario, we find that the parameters required to represent the high state are significantly different than those in the low state. Motivated by possible evidence of gas in the vicinity of the blazar, we also investigate a reflected emission model to describe the observed variability pattern. This model assumes that the non-thermal emission from the jet is reflected by a nearby cloud of gas, allowing the reflected emission to re-enter the blob and produce an elevated gamma-ray state with no simultaneous elevated synchrotron flux. The model applied here, although not required to explain the observed variability pattern, represents one possible scenario which can describe the observations. As applied to an elevated VHE state of 66% of the Crab Nebula flux, observed on a single night during the observation period, the reflected emission scenario does not support a purely leptonic non-thermal emission mechanism. The reflected emission model does, however, predict a reflected photon field with sufficient energy to enable elevated gamma-ray emission via pion production with protons of energies between 10 and 100 TeV.

  11. Intraoperative Schnellschnittuntersuchungen parapylorischer Lymphknoten bei der pyloruserhaltenden Pankreaskopfresektion: Gibt es eine klinische Relevanz?

    PubMed Central

    Riediger, Hartwig; Schulz, Antje; Adam, Ulrich; Krüger, Colin M.

    2014-01-01

    Zusammenfassung Hintergrund Die pyloruserhaltende Pankreaskopfresektion (PPPD) ist als onkologisches Standardverfahren etabliert. Lokal fortgeschrittene Tumoren können eine erweiterte Resektion erforderlich machen. Ebenso soll früheren Arbeiten zufolge bei Tumornachweis in den parapylorischen Lymphknoten (PLK) eine distale Magenresektion im Sinne einer klassischen Whipple-Operation indiziert sein. Entsprechend diesen Empfehlungen haben wir intraoperative Schnellschnittuntersuchungen der PLK in unseren Routineablauf integriert. Im Rahmen dieser Studie haben wir die klinische Relevanz dieses Vorgehens hinterfragt. Methoden Bei 105 onkologischen Patienten im Zeitraum von 2006-2012 bestand die Indikation zur PPPD. In allen Fällen erfolgte eine intraoperative Schnellschnittuntersuchung der PLK. Die Patienten wurden bezüglich Primärtumor, Anzahl der untersuchten Lymphknoten (LK) (gesamt und parapylorisch) sowie Auswirkungen auf das operative Konzept untersucht. Es handelt sich um eine retrospektive Studie, die auf prospektiv erhobenen Daten unserer Pankreasdatenbank basiert. Ergebnisse Die Primärtumoren waren 72 Pankreaskopfkarzinome und 33 extrapankreatische Karzinome (Gallengangskarzinom, Ampullenkarzinom, Duodenalkarzinom). 73 Patienten waren nodalpositiv. Insgesamt wurden 2391 LK untersucht, von denen 325 parapylorisch lokalisiert waren. Die intraoperative Schnellschnittuntersuchung erbrachte lediglich bei 4 Patienten mit Pankreaskopfkarzinom jeweils einen positiven PLK; daraufhin erfolgte eine distale Magenresektion. In keinem der distalen Magenresektate waren Tumorresiduen nachweisbar. Lokale chirurgisch-technische Probleme im Sinne von Durchblutungsstörungen des Magens ergaben sich durch die regionale Lymphadenektomie nicht. PLK waren nur beim Pankreaskarzinom positiv. In der Subgruppe der nodalpositiven Patienten mit Pankreaskopfkarzinom hatten 8% der Patienten einen positiven PLK. Schlussfolgerung Die regionale parapylorische Lymphadenektomie ist beim

  12. Imagerie médicale avec des sources X créées parlaser

    NASA Astrophysics Data System (ADS)

    Dorchies, F.; Chen, L. M.; Ichalalene, Z.; Jiang, Z.; Kieffer, J. C.; Chamberlain, C. C.; Krol, A.

    2003-06-01

    L'interaction d'une impulsion laser femtoseconde avec une cible solide peut induire une émission intense de rayonnement X dans la gamme de quelques dizaines de keV. Ce type de source présente potentiellement plusieurs avantages sur les installations X conventionnelles en termes de performances (taille de la source, ganinie de longueur d'onde accessible) et de coût. Ce papier réunit les différents résultats obtenus dans le cadre du projet d'imagerie médicale de I'INRS à Varennes, Québec, Canada. Ces travaux concernent d'une part l'étude quantitative de la source de rayons X-durs: intensité, spectre et taille de la source. D'autre part. des expériences de faisabilité ont montré la pertinence de leur application à l'imagerie médicale. notamment dans le domaine de l'imagerie à grande résolution spatiale (mammographie) et dans celui de l'imagerie par soustraction d'images A deux longueurs d'onde (angiographie cardiaque). La principale limitation de ces expériences était la puissance moyenne de la source bridée par la faible cadence de l'installation laser. Les lois d'échelle déduites des expériences présentées laissent présager la poursuite de ces travaux dans des conditions cliniques réalistes avec le développement actuel de chaîne laser de hautes cadences (100Hz 1kHz).

  13. Health-care worker engagement in HIV-related quality improvement in Dar es Salaam, Tanzania

    PubMed Central

    Garcia, Maria E.; Li, Michelle S.; Siril, Hellen; Hawkins, Claudia; Kaaya, Sylvia; Ismail, Shabbir; Chalamilla, Guerino; Mdingi, Sarah Geoffrey; Hirschhorn, Lisa R.

    2011-01-01

    Objective To assess health-care worker (HCW) awareness, interest and engagement in quality improvement (QI) in HIV care sites in Tanzania. Design Cross-sectional survey distributed in May 2009. Setting Sixteen urban HIV care sites in Dar es Salaam, Tanzania, 1 year after the introduction of a quality management program. Participants Two hundred seventy-nine HCWs (direct care, clinical support staff and management). Main Outcome Measures HCW perceptions of care delivered, rates of engagement, knowledge and interest in QI. HCW-identified barriers to and facilitators of the delivery of quality HIV care. Results Two hundred seventy-nine (73%) of 382 HCWs responded to the survey. Most (86%) felt able to meet clients’ needs. HCW-identified facilitators of quality included: teamwork (88%), staff communication (79%), positive work environment (75%) and trainings (84%). Perceived barriers included: problems in patients’ lives (73%) and too few staff or too high patient volumes (52%). Many HCWs knew about specific QI activities (52%) or had been asked for input on QI (63%), but fewer (40.5%) had participated in activities and only 20.1% were currently QI team members. Managers were more likely to report QI involvement than direct care or clinical support staff (P < 0.01). No difference in QI involvement was seen based on patient load or site type. Conclusions HCWs can provide important insights into barriers and facilitators of providing quality care and can be effectively engaged in QI activities. HCW participation in efforts to improve services will ensure that HIV/AIDS quality of care is achieved and maintained as countries strive for universal antiretroviral access. PMID:21441571

  14. Microbial Efficacy of Waterless Hand Hygiene in Dar es Salaam, Tanzania

    NASA Astrophysics Data System (ADS)

    Pickering, A.; Boehm, A.; Davis, J.

    2008-12-01

    Millions of people die from diarrheal and respiratory diseases every year due to lack of proper sanitation, hygiene, and access to clean water. The act of handwashing with soap has been found to effectively reduce both diarrheal and respiratory illness, however, handwashing at critical times (i.e. after using the toilet, before preparing food) remains infrequent around the world. This research investigates the potential for alcohol- based hand sanitizer (ABHS) to be an effective and appropriate hand hygiene option in developing countries. A study was conducted to assess the microbiological effectiveness of ABHS, as compared to handwashing with soap and water, in field conditions in Dar es Salaam, Tanzania. A total of 205 participants, including mothers, nurses, students, and teachers, were introduced to ABHS, given a standardized amount (2ml) of product, and instructed on how to use the product correctly. Hand samples were obtained using the hand rinse method before and after the use of ABHS from 152 participants. The other 53 participants were hand sampled before and after handwashing with a non-antimicrobial liquid soap and clean water (prior to using ABHS). Visual inspections of the hands were performed before hand sampling to record the level of dirt on the hands. All hand samples were processed and analyzed by membrane filtration for concentrations of two microbial indicators, enterococci and E. coli. User perceptions of the product and willingness to pay are also documented. The results of this study provide valuable insight on the prospective of promoting ABHS in developing countries and water scarce areas.

  15. COST Action ES1401 TIDES: a European network on TIme DEpendent Seismology

    NASA Astrophysics Data System (ADS)

    Morelli, Andrea

    2016-04-01

    Using the full-length records of seismic events and background ambient noise, today seismology is going beyond still-life snapshots of the interior of the Earth, and look into time-dependent changes of its properties. Data availability has grown dramatically with the expansion of seismographic networks and data centers, so as to enable much more detailed and accurate analyses. COST Action ES1401 TIDES (TIme DEpendent Seismology; http://tides-cost.eu) aims at structuring the EU seismological community to enable development of data-intensive, time-dependent techniques for monitoring Earth active processes (e.g., earthquakes, volcanic eruptions, landslides, glacial earthquakes) as well as oil/gas reservoirs. The main structure of TIDES is organised around working groups on: Workflow integration of data and computing resources; Seismic interferometry and ambient noise; Forward problems and High-performance computing applications; Seismic tomography, full waveform inversion and uncertainties; Applications in the natural environment and industry. TIDES is an open network of European laboratories with complementary skills, and is organising a series of events - workshops and advanced training schools - as well as supporting short-duration scientific stays. The first advanced training school was held in Bertinoro (Italy) on June 2015, with attendance of about 100 participants from 20 European countries, was devoted to how to manage and model seismic data with modern tools. The next school, devoted to ambient noise, will be held in 2016 Portugal: the program will be announced at the time of this conference. TIDES will strengthen Europe's role in a critical field for natural hazards and natural resource management.

  16. Investigating broadband variability of the TeV blazar 1ES 1959+650

    DOE PAGES

    Aliu, E.; Archambault, S.; Arlen, T.; Aune, T.; Barnacka, A.; Beilicke, M.; Benbow, W.; Berger, K.; Bird, R.; Bouvier, A.; et al

    2014-12-03

    We summarize broadband observations of the TeV-emitting blazar 1ES 1959 650, including optical R-band observations by the robotic telescopes Super-LOTIS and iTelescope, UV observations by Swift UVOT, X-ray observations by the Swift X-ray Telescope, high-energy gamma-ray observations with the Fermi Large Area Telescope, and very-high-energy (VHE) gamma-ray observations by VERITAS above 315 GeV, all taken between 2012 April 17 and 2012 June 1 (MJD 56034 and 56079). The contemporaneous variability of the broadband spectral energy distribution is explored in the context of a simple synchrotron self Compton (SSC) model. In the SSC emission scenario, we find that the parameters requiredmore » to represent the high state are significantly different than those in the low state. Motivated by possible evidence of gas in the vicinity of the blazar, we also investigate a reflected emission model to describe the observed variability pattern. This model assumes that the non-thermal emission from the jet is reflected by a nearby cloud of gas, allowing the reflected emission to re-enter the blob and produce an elevated gamma-ray state with no simultaneous elevated synchrotron flux. The model applied here, although not required to explain the observed variability pattern, represents one possible scenario which can describe the observations. As applied to an elevated VHE state of 66% of the Crab Nebula flux, observed on a single night during the observation period, the reflected emission scenario does not support a purely leptonic non-thermal emission mechanism. The reflected model does, however, predict a reflected photon field with sufficient energy to enable elevated gamma-ray emission via pion production with protons of energies between 10 and 100 TeV.« less

  17. Investigating broadband variability of the TeV blazar 1ES 1959+650

    SciTech Connect

    Aliu, E.; Archambault, S.; Arlen, T.; Aune, T.; Barnacka, A.; Beilicke, M.; Benbow, W.; Berger, K.; Bird, R.; Bouvier, A.; Buckley, J. H.; Bugaev, V.; Cerruti, M.; Chen, X.; Ciupik, L.; Collins-Hughes, E.; Connolly, M. P.; Cui, W.; Dumm, J.; Eisch, J. D.; Falcone, A.; Federici, S.; Feng, Q.; Finley, J. P.; Fleischhack, H.; Fortin, P.; Fortson, L.; Furniss, A.; Galante, N.; Gillanders, G. H.; Griffin, S.; Griffiths, S. T.; Grube, J.; Gyuk, G.; Håkansson, N.; Hanna, D.; Holder, J.; Hughes, G.; Hughes, Z.; Humensky, T. B.; Johnson, C. A.; Kaaret, P.; Kar, P.; Kertzman, M.; Khassen, Y.; Kieda, D.; Krawczynski, H.; Krennrich, F.; Lang, M. J.; Madhavan, A. S.; Majumdar, P.; McArthur, S.; McCann, A.; Meagher, K.; Millis, J.; Moriarty, P.; Mukherjee, R.; Nelson, T.; Nieto, D.; de Bhróithe, A. O'Faoláin; Ong, R. A.; Otte, A. N.; Park, N.; Perkins, J. S.; Pohl, M.; Popkow, A.; Prokoph, H.; Quinn, J.; Ragan, K.; Rajotte, J.; Reyes, L. C.; Reynolds, P. T.; Richards, G. T.; Roache, E.; Sadun, A.; Santander, M.; Sembroski, G. H.; Shahinyan, K.; Sheidaei, F.; Smith, A. W.; Staszak, D.; Telezhinsky, I.; Theiling, M.; Tyler, J.; Varlotta, A.; Vassiliev, V. V.; Vincent, S.; Wakely, S. P.; Weekes, T. C.; Weinstein, A.; Welsing, R.; Wilhelm, A.; Williams, D. A.; Zitzer, and B.; Böttcher, M.; Fumagalli, M.

    2014-12-03

    We summarize broadband observations of the TeV-emitting blazar 1ES 1959 650, including optical R-band observations by the robotic telescopes Super-LOTIS and iTelescope, UV observations by Swift UVOT, X-ray observations by the Swift X-ray Telescope, high-energy gamma-ray observations with the Fermi Large Area Telescope, and very-high-energy (VHE) gamma-ray observations by VERITAS above 315 GeV, all taken between 2012 April 17 and 2012 June 1 (MJD 56034 and 56079). The contemporaneous variability of the broadband spectral energy distribution is explored in the context of a simple synchrotron self Compton (SSC) model. In the SSC emission scenario, we find that the parameters required to represent the high state are significantly different than those in the low state. Motivated by possible evidence of gas in the vicinity of the blazar, we also investigate a reflected emission model to describe the observed variability pattern. This model assumes that the non-thermal emission from the jet is reflected by a nearby cloud of gas, allowing the reflected emission to re-enter the blob and produce an elevated gamma-ray state with no simultaneous elevated synchrotron flux. The model applied here, although not required to explain the observed variability pattern, represents one possible scenario which can describe the observations. As applied to an elevated VHE state of 66% of the Crab Nebula flux, observed on a single night during the observation period, the reflected emission scenario does not support a purely leptonic non-thermal emission mechanism. The reflected model does, however, predict a reflected photon field with sufficient energy to enable elevated gamma-ray emission via pion production with protons of energies between 10 and 100 TeV.

  18. Further developments of 8μm pitch MCT pixels at Finmeccanica (formerly Selex ES)

    NASA Astrophysics Data System (ADS)

    Jeckells, David; McEwen, R. Kennedy; Bains, Sudesh; Herbert, Martin

    2016-05-01

    Finmeccanica (formerly Selex ES) introduced high performance mercury cadmium telluride (MCT) infrared detectors on an 8μm pitch in 2015 with their SuperHawk device which builds on standard production processes already used for the manufacture of 24μm, 20μm, 16μm and 12μm pitch devices. The flexibility of the proprietary Finmeccanica designed diode structure, used in conjunction with the mature production Metal Organic Vapour Phase Epitaxy (MOVPE) MCT growth process at Finmeccanica, enables fine control of diode electrical and optical structure including free choice of cut-off wavelength. The mesa pixel design inherently provides major system performance benefits by reducing blurring mechanisms, including optical scattering, inter-pixel cross-talk and carrier diffusion, to negligible levels. The SuperHawk detector has demonstrated unrivalled MTF and NETD performance, even when operating at temperatures in excess of 120K. The SuperHawk Integrated Detector Cooler Assembly (IDCA) benefits from recent dewar developments at Finmeccanica, which have improved thermal efficiencies while maintaining mechanical integrity over a wide range of applications, enabling use of smaller cryo-coolers to reduce system SWAP-C. Performance and qualification results are presented together with example imagery. SuperHawk provides an easy high resolution upgrade for systems currently based on standard definition 16μm and 15μm infrared detector formats. The paper also addresses further work to increase the operating temperature of the established 8μm process, exploiting High Operating Temperature (HOT) MCT at Finmeccanica, as well as options for LWIR variants of the SuperHawk device.

  19. Classification of methods in transcranial Electrical Stimulation (tES) and evolving strategy from historical approaches to contemporary innovations

    PubMed Central

    Guleyupoglu, Berkan; Schestatsky, Pedro; Edwards, Dylan; Fregni, Felipe; Bikson, Marom

    2013-01-01

    Transcranial Electrical Stimulation (tES) encompasses all methods of non-invasive current application to the brain used in research and clinical practice. We present the first comprehensive and technical review, explaining the evolution of tES in both terminology and dosage over the past 100 years of research to present day. Current transcranial Pulsed Current Stimulation (tPCS) approaches such as Cranial Electrotherapy Stimulation (CES) descended from Electrosleep (ES) through Cranial Electro-stimulation Therapy (CET), Transcerebral Electrotherapy (TCET), and NeuroElectric Therapy (NET) while others like Transcutaneous Cranial Electrical Stimulation (TCES) descended from Electroanesthesia (EA) through Limoge, and Interferential Stimulation. Prior to a contemporary resurgence in interest, variations of trans-cranial Direct Current Stimulation were explored intermittently, including Polarizing current, Galvanic Vestibular Stimulation (GVS), and Transcranial Micropolarization. The development of these approaches alongside Electroconvulsive Therapy (ECT) and pharmacological developments are considered. Both the roots and unique features of contemporary approaches such as transcranial Alternating Current Stimulation (tACS) and transcranial Random Noise Stimulation (tRNS) are discussed. Trends and incremental developments in electrode montage and waveform spanning decades are presented leading to the present day. Commercial devices, seminal conferences, and regulatory decisions are noted. We conclude with six rules on how increasing medical and technological sophistication may now be leveraged for broader success and adoption of tES. PMID:23954780

  20. National and Global: A History of Scholars' Experiences with Research at the University of Dar Es Salaam, Tanzania (1961-Present)

    ERIC Educational Resources Information Center

    Jamison, Amy J.

    2010-01-01

    In this dissertation, I draw on research carried out at the University of Dar es Salaam (UDSM), Tanzania in 2008 to examine Tanzanian academics' experience with research throughout the history of this institution. This dissertation is designed as an historical case study and investigates how economic and political changes in Tanzania's…

  1. Intimate Partner Violence and the Association with HIV Risk Behaviors among Young Men in Dar Es Salaam, Tanzania

    ERIC Educational Resources Information Center

    Maman, Suzanne; Yamanis, Thespina; Kouyoumdjian, Fiona; Watt, Melissa; Mbwambo, Jessie

    2010-01-01

    There is growing evidence of the association between gender-based violence and HIV from the perspective and experiences of women. The purpose of this study is to examine these associations from the perspective of young men living in Dar es Salaam, Tanzania. A community-based sample of 951 men were interviewed, of whom 360 had sex in the past 6…

  2. Influence of Carbon Sources and Electron Shuttles on Ferric Iron Reduction by Cellulomonas sp. Strain ES6

    SciTech Connect

    Dr Robin Gerlach; Erin K. Field; Sridhar Viamajala; Brent M. Peyton; William A. Apel; Al B. Cunningham

    2011-09-01

    Microbially reduced iron minerals can reductively transform a variety of contaminants including heavy metals, radionuclides, chlorinated aliphatics, and nitroaromatics. A number of Cellulomonas spp. strains, including strain ES6, isolated from aquifer samples obtained at the U.S. Department of Energy's Hanford site in Washington, have been shown to be capable of reducing Cr(VI), TNT, natural organic matter, and soluble ferric iron [Fe(III)]. This research investigated the ability of Cellulomonas sp. strain ES6 to reduce solid phase and dissolved Fe(III) utilizing different carbon sources and various electron shuttling compounds. Results suggest that Fe(III) reduction by and growth of strain ES6 was dependent upon the type of electron donor, the form of iron present, and the presence of synthetic or natural organic matter, such as anthraquinone-2,6-disulfonate (AQDS) or humic substances. This research suggests that Cellulomonas sp. strain ES6 could play a significant role in metal reduction in the Hanford subsurface and that the choice of carbon source and organic matter addition can allow for independent control of growth and iron reduction activity.

  3. Influence of carbon sources and electron shuttles on ferric iron reduction by Cellulomonas sp. strain ES6.

    PubMed

    Gerlach, Robin; Field, Erin K; Viamajala, Sridhar; Peyton, Brent M; Apel, William A; Cunningham, Al B

    2011-09-01

    Microbially reduced iron minerals can reductively transform a variety of contaminants including heavy metals, radionuclides, chlorinated aliphatics, and nitroaromatics. A number of Cellulomonas spp. strains, including strain ES6, isolated from aquifer samples obtained at the U.S. Department of Energy's Hanford site in Washington, have been shown to be capable of reducing Cr(VI), TNT, natural organic matter, and soluble ferric iron [Fe(III)]. This research investigated the ability of Cellulomonas sp. strain ES6 to reduce solid phase and dissolved Fe(III) utilizing different carbon sources and various electron shuttling compounds. Results suggest that Fe(III) reduction by and growth of strain ES6 was dependent upon the type of electron donor, the form of iron present, and the presence of synthetic or natural organic matter, such as anthraquinone-2,6-disulfonate (AQDS) or humic substances. This research suggests that Cellulomonas sp. strain ES6 could play a significant role in metal reduction in the Hanford subsurface and that the choice of carbon source and organic matter addition can allow for independent control of growth and iron reduction activity.

  4. Detection of Very High Energy Gamma-Ray Emission from 1ES 2037+521 with the MAGIC telescopes

    NASA Astrophysics Data System (ADS)

    Mirzoyan, Razmik

    2016-10-01

    The MAGIC collaboration reports the detection of very high energy (VHE) gamma-ray emission from 1ES 2037+521 (RA 20h39m23.5s, DEC +52d19m50s, J2000.0). The object was observed with the MAGIC telescopes for 11 hours between 2016/09/26 and 2016/09/30.

  5. The TeV HBL blazar 1ES 1011+496 in Gamma-, X-, Optical flaring activity

    NASA Astrophysics Data System (ADS)

    Pacciani, Luigi; Stamerra, Antonio

    2015-03-01

    We detected a gamma-ray flare from the TeV HBL blazar 1ES 1011+496 (z=0.212), triggering on FERMI-LAT data at E > 10 GeV with TS ~50, from 2015-03-12 to 2015-03-16, following the prescription of Pacciani et al.

  6. Numerical simulations of oceanic oxygen cycling in the FAMOUS Earth-System model: FAMOUS-ES, version 1.0

    NASA Astrophysics Data System (ADS)

    Williams, J. H. T.; Totterdell, I. J.; Halloran, P. R.; Valdes, P. J.

    2014-02-01

    Addition and validation of an oxygen cycle to the ocean component of the FAMOUS climate model are described. Surface validation is carried out with respect to HadGEM2-ES where good agreement is found and where discrepancies are mainly attributed to disagreement in surface temperature structure between the models. The agreement between the models at depth (where observations are also used in the comparison) in the Southern Hemisphere is less encouraging than in the Northern Hemisphere. This is attributed to a combination of excessive surface productivity in FAMOUS' equatorial waters (and its concomitant effect on remineralisation at depth) and its reduced overturning circulation compared to HadGEM2-ES. For the entire Atlantic basin FAMOUS has a circulation strength of 12.7 ± 0.4 Sv compared to 15.0 ± 0.9 for HadGEM2-ES. The HadGEM2-ES data used in this paper were obtained from the online database of the fifth Coupled Model Intercomparison Project, CMIP5 (Taylor et al., 2012).

  7. Transit Timing Variations of TrES-2: a combined analysis of ground- and space-based photometry

    NASA Astrophysics Data System (ADS)

    Raetz, St.; Ginski, Ch.; Mugrauer, M.; Berndt, A.; Eisenbeiss, T.; Adam, Ch.; Raetz, M.; Roell, T.; Seeliger, M.; Maciejewski, G.; Marka, C.; Vanko, M.; Bukowiecki, L.; Errmann, R.; Kitze, M.; Ohlert, J.; Pribulla, T.; Schmidt, J. G.; Sebastian, D.; Tetzlaff, N.; Hohle, M. M.; Schmidt, T. O. B.; Neuhäuser, R.

    2013-07-01

    TrES-2 is one of the few exoplanets, which offer the matchless possibility to combine long-term ground-based observations with continuous satellite data. TrES-2 is a target of our "Transit Timing Variations @ YETI" (TTV@YETI) project which is dedicated to detect and characterize signals of transit timing variations. We observed 43 ground-based light curves of 30 individual transit events of TrES-2. We used seven 0.2 - 2.2m telescopes located at five observatories in Germany and Spain. In addition, we analyzed 16 quarters (Q0-Q15) of observational data from NASA's space telescope Kepler including 424 individual transit events. The continuous monitoring of Kepler allows to determine the system parameters of each of the 424 transits and search for possible changes of these quantities. Here we present a first indication of an increasing stellar activity of the TrES-2 host star and provide new limits on possible transit timing variations.

  8. Angular distribution of {alpha} particles from oriented {sup 253,254}Es and {sup 255}Fm nuclei

    SciTech Connect

    Severijns, N.; Golovko, V.V.; Kraev, I.S.; Phalet, T.; Belyaev, A.A.; Lukhanin, A.A.; Noga, V.I.; Erzinkyan, A.L.; Parfenova, V.P.; Eversheim, P.-D.; Herzog, P.; Tramm, C.; Filimonov, V.T.; Toporov, Yu.G.; Zotov, E.; Gurevich, G.M.; Rusakov, A.V.; Vyachin, V.N.; Zakoucky, D.

    2005-04-01

    The anisotropy in the angular distribution of {alpha} particles from oriented {sup 253,254}Es and {sup 255}Fm nuclei, which are among the strongest deformed {alpha} emitters, was measured. Large {alpha} anisotropies have been observed for all three nuclei. The results are compared with calculations based on {alpha}-particle tunneling through a deformed Coulomb barrier.

  9. The Effectiveness of Texas Teacher Certification Programs as Evidenced through Pass Rates on TExES PPR 160 Exam

    ERIC Educational Resources Information Center

    Clouse, Scarlet

    2013-01-01

    The purpose of this study was to investigate the differences between alternative certification pathways, specifically those offered through a university-based, post baccalaureate certification program and a regional education service center certification program. A quantitative research design was implemented and archived scores on the TExES PPR…

  10. Classification of methods in transcranial electrical stimulation (tES) and evolving strategy from historical approaches to contemporary innovations.

    PubMed

    Guleyupoglu, Berkan; Schestatsky, Pedro; Edwards, Dylan; Fregni, Felipe; Bikson, Marom

    2013-10-15

    Transcranial Electrical Stimulation (tES) encompasses all methods of non-invasive current application to the brain used in research and clinical practice. We present the first comprehensive and technical review, explaining the evolution of tES in both terminology and dosage over the past 100 years of research to present day. Current transcranial Pulsed Current Stimulation (tPCS) approaches such as Cranial Electrotherapy Stimulation (CES) descended from Electrosleep (ES) through Cranial Electro-stimulation Therapy (CET), Transcerebral Electrotherapy (TCET), and NeuroElectric Therapy (NET) while others like Transcutaneous Cranial Electrical Stimulation (TCES) descended from Electroanesthesia (EA) through Limoge, and Interferential Stimulation. Prior to a contemporary resurgence in interest, variations of transcranial Direct Current Stimulation were explored intermittently, including Polarizing current, Galvanic Vestibular Stimulation (GVS), and Transcranial Micropolarization. The development of these approaches alongside Electroconvulsive Therapy (ECT) and pharmacological developments are considered. Both the roots and unique features of contemporary approaches such as transcranial Alternating Current Stimulation (tACS) and transcranial Random Noise Stimulation (tRNS) are discussed. Trends and incremental developments in electrode montage and waveform spanning decades are presented leading to the present day. Commercial devices, seminal conferences, and regulatory decisions are noted. We conclude with six rules on how increasing medical and technological sophistication may now be leveraged for broader success and adoption of tES.

  11. 75 FR 5931 - Anthropomorphic Test Devices; Hybrid III Test Dummy, ES-2re Side Impact Crash Test Dummy

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-05

    ..., 2000 (65 FR 19477-78). Docket: For access to the docket to read background documents or comments... published a final rule on June 16, 2008 (73 FR 33903, Docket No. NHTSA-08-0111) that responded to various... adopting the ES-2re into 49 CFR part 572 was published December 14, 2006 (71 FR 75303, Docket No....

  12. 76 FR 31860 - Anthropomorphic Test Devices; Hybrid III Test Dummy, ES-2re Side Impact Crash Test Dummy

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-02

    .... SUPPLEMENTARY INFORMATION: I. Background NHTSA published a final rule on June 16, 2008 (73 FR 33903, Docket No... adopting the ES-2re into 49 CFR part 572 was published December 14, 2006 (71 FR 75303, Docket No. NHTSA-04... notice of proposed rulemaking (NPRM) published February 5, 2010 (75 FR 5931; Docket No....

  13. The GAPS programme with HARPS-N at TNG. VI. The curious case of TrES-4b

    NASA Astrophysics Data System (ADS)

    Sozzetti, A.; Bonomo, A. S.; Biazzo, K.; Mancini, L.; Damasso, M.; Desidera, S.; Gratton, R.; Lanza, A. F.; Poretti, E.; Rainer, M.; Malavolta, L.; Affer, L.; Barbieri, M.; Bedin, L. R.; Boccato, C.; Bonavita, M.; Borsa, F.; Ciceri, S.; Claudi, R. U.; Gandolfi, D.; Giacobbe, P.; Henning, T.; Knapic, C.; Latham, D. W.; Lodato, G.; Maggio, A.; Maldonado, J.; Marzari, F.; Martinez Fiorenzano, A. F.; Micela, G.; Molinari, E.; Mordasini, C.; Nascimbeni, V.; Pagano, I.; Pedani, M.; Pepe, F.; Piotto, G.; Santos, N.; Scandariato, G.; Shkolnik, E.; Southworth, J.

    2015-03-01

    We update the TrES-4 system parameters using high-precision HARPS-N radial-velocity measurements and new photometric light curves. A combined spectroscopic and photometric analysis allows us to determine a spectroscopic orbit with a semi-amplitude K = 51 ± 3 m s-1. The derived mass of TrES-4b is found to be Mp = 0.49 ± 0.04 MJup, significantly lower than previously reported. Combined with the large radius () inferred from our analysis, TrES-4b becomes the transiting hot Jupiter with the second-lowest density known. We discuss several scenarios to explain the puzzling discrepancy in the mass of TrES-4b in the context of the exotic class of highly inflated transiting giant planets. Based on observations made with the Italian Telescopio Nazionale Galileo (TNG) operated on the island of La Palma by the Fundacion Galileo Galilei of the INAF at the Spanish Observatorio del Roque de los Muchachos of the IAC in the frame of the program Global Architecture of Planetary Systems (GAPS), and with the Zeiss 1.23-m telescope at the German-Spanish Astronomical Center at Calar Alto, Spain. Tables 1 and 3 are available in electronic form at http://www.aanda.org

  14. FACT and MAGIC measure an increased gamma-ray flux from the HBL 1ES 1959+650

    NASA Astrophysics Data System (ADS)

    Biland, A.; Mirzoyan, R.

    2016-07-01

    The FACT and MAGIC collaborations report the measurement of an enhanced gamma-ray flux at about 1 TeV from a position consistent with the HBL 1ES 1959+650 (z=0.047, Schachter et al. 1993, ApJ, 412, 541).

  15. ATMOSPHERE AND SPECTRAL MODELS OF THE KEPLER-FIELD PLANETS HAT-P-7b AND TrES-2

    SciTech Connect

    Spiegel, David S.; Burrows, Adam E-mail: burrows@astro.princeton.ed

    2010-10-10

    We develop atmosphere models of two of the three Kepler-field planets that were known prior to the start of the Kepler mission (HAT-P-7b and TrES-2). We find that published Kepler and Spitzer data for HAT-P-7b appear to require an extremely hot upper atmosphere on the dayside, with a strong thermal inversion and little day-night redistribution. The Spitzer data for TrES-2 suggest a mild thermal inversion with moderate day-night redistribution. We examine the effect of nonequilibrium chemistry on TrES-2 model atmospheres and find that methane levels must be adjusted by extreme amounts in order to cause even mild changes in atmospheric structure and emergent spectra. Our best-fit models to the Spitzer data for TrES-2 lead us to predict a low secondary eclipse planet-star flux ratio ({approx}<2 x 10{sup -5}) in the Kepler bandpass, which is consistent with what very recent observations have found. Finally, we consider how the Kepler-band optical flux from a hot exoplanet depends on the strength of a possible extra optical absorber in the upper atmosphere. We find that the optical flux is not monotonic in optical opacity, and the non-monotonicity is greater for brighter, hotter stars.

  16. Measuring Students' Emotions in the Early Years: The Achievement Emotions Questionnaire-Elementary School (AEQ-ES)

    ERIC Educational Resources Information Center

    Lichtenfeld, Stephanie; Pekrun, Reinhard; Stupnisky, Robert H.; Reiss, Kristina; Murayama, Kou

    2012-01-01

    This article reports about the development and validation of a measurement instrument assessing elementary school students' achievement emotions (Achievement Emotions Questionnaire-Elementary School, AEQ-ES). Specifically, the instrument assesses students' enjoyment, anxiety, and boredom pertaining to three types of academic settings (i.e.,…

  17. Provision of Vocational Skills Education to Orphans: Lessons from Orphanage Centres in Dar es Salaam City, Tanzania

    ERIC Educational Resources Information Center

    Meli, Benjamin Mbeba

    2015-01-01

    This paper utilises data from a study that investigated the efficacy of vocational skills training provided to orphans from three orphanages in Temeke District, Dar es Salaam. The three orphanage centres that were studied are Kurasini National Children Home, Saudia and Don Bosco Vocational Centre. The sample comprised of 45 orphans, an official…

  18. 77 FR 42831 - Professional Transportation, Inc.-Asset Acquisition-CUSA ES, LLC and CUSA CSS, LLC

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-20

    ... published in the Federal Register (77 FR 38884-85). The notice contained an omission in the address... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Surface Transportation Board Professional Transportation, Inc.--Asset Acquisition--CUSA ES, LLC and...

  19. Expression of human BRCA1 variants in mouse ES cells allows functional analysis of BRCA1 mutations.

    PubMed

    Chang, Suhwan; Biswas, Kajal; Martin, Betty K; Stauffer, Stacey; Sharan, Shyam K

    2009-10-01

    To date, inheritance of a mutant BRCA1 or BRCA2 gene is the best-established indicator of an increased risk of developing breast cancer. Sequence analysis of these genes is being used to identify BRCA1/2 mutation carriers, though these efforts are hampered by the high frequency of variants of unknown clinical significance (VUSs). Functional evaluation of such variants has been restricted due to lack of a physiologically relevant assay. In this study we developed a functional assay using mouse ES cells to study variants of BRCA1. We introduced BAC clones with human wild-type BRCA1 or variants into Brca1-null ES cells and confirmed that only wild-type and a known neutral variant rescued cell lethality. The same neutral variant was also able to rescue embryogenesis in Brca1-null mice. A test of several BRCT domain mutants revealed all to be deleterious, including a VUS. Furthermore, we used this assay to determine the effects of BRCA1 variants on cell cycle regulation, differentiation, and genomic stability. Importantly, we discovered that ES cells rescued by S1497A BRCA1 exhibited significant hypersensitivity after gamma-irradiation. Our results demonstrate that this ES cell-based assay is a powerful and reliable method for analyzing the functional impact of BRCA1 variants, which we believe could be used to determine which patients may require preventative treatments. PMID:19770520

  20. 76 FR 5185 - National Institute of Environmental Health Sciences (NIEHS); Request for Information (NOT-ES-11...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-28

    ...); Request for Information (NOT-ES-11-006): Interagency Breast Cancer and Environmental Research Coordinating... (NIH). ACTION: Request for information. SUMMARY: The Interagency Breast Cancer and Environmental... advocates for individuals with breast cancer. The IBCERCC is charged with reviewing all research...

  1. A Trainer's Guide to El Comienzo & Esto Es Familiar: Keys to Training Spanish-Speaking Family Day Care Providers.

    ERIC Educational Resources Information Center

    Bellm, Dan

    This set of manuals was developed by the California Child Care Initiative for the training of Spanish-speaking family day care providers. The English-language trainer's guide has been designed to accompany "El Comienzo" ("The Beginner") and "Esto Es Familiar" ("It's a Family Affair"). The Spanish-language "El Comienzo" is an information kit…

  2. VizieR Online Data Catalog: CHANG-ES. IV. VLA D-configuration observations (Wiegert+, 2015)

    NASA Astrophysics Data System (ADS)

    Wiegert, T.; Irwin, J.; Miskolczi, A.; Schmidt, P.; Mora, S. C.; Damas-Segovia, A.; Stein, Y.; English, J.; Rand, R. J.; Santistevan, I.; Walterbos, R.; Krause, M.; Beck, R.; Dettmar, R.-J.; Kepley, A.; Wezgowiec, M.; Wang, Q. D.; Heald, G.; Li, J.; MacGregor, S.; Johnson, M.; Strong, A. W.; Desouza, A.; Porter, T. A.

    2016-04-01

    This is the fourth paper in the series "Continuum Halos in Nearby Galaxies, an EVLA Survey" (CHANG-ES). With CHANG-ES, we have observed 35 nearby edge-on galaxies in the radio continuum in L- and C-bands (centered at approximately 1.5 and 6GHz, respectively), in three array configurations (B, C, D; in the B configuration only L-band was observed) of the Karl G. Jansky Very Large Array (hereafter VLA). We refer to Table1 of Irwin et al. (2012AJ....144...43I; Paper I) for details of the galaxy sample. Two other papers, Irwin et al. (2012AJ....144...44I; Paper II) and Irwin et al. (2013AJ....146..164I; Paper III), present the detailed results of CHANG-ES observations of NGC 4631 and UGC 10288, respectively. In this, the fourth CHANG-ES paper, we present all observations that were carried out in the shortest baseline array configuration, D. The data products (including intensity maps, spectral index maps, and polarization maps) are part of our Data Release 1, located at http://www.queensu.ca/changes. Of the 405hr that were awarded for the entire CHANG-ES project, 65hr were set apart for the D-configuration observations in two frequency bands, L and C. The observations were divided up into 13 scheduling blocks. The bulk of the data were observed during 2011 December. Two scheduling blocks were reobserved in 2013 March. The details of the observations are presented in Table1. (5 data files).

  3. MAGIC detection of short-term variability of the high-peaked BL Lac object 1ES 0806+524

    NASA Astrophysics Data System (ADS)

    Aleksić, J.; Ansoldi, S.; Antonelli, L. A.; Antoranz, P.; Babic, A.; Bangale, P.; Barrio, J. A.; Becerra González, J.; Bednarek, W.; Bernardini, E.; Biasuzzi, B.; Biland, A.; Blanch, O.; Bonnefoy, S.; Bonnoli, G.; Borracci, F.; Bretz, T.; Carmona, E.; Carosi, A.; Colin, P.; Colombo, E.; Contreras, J. L.; Cortina, J.; Covino, S.; Da Vela, P.; Dazzi, F.; De Angelis, A.; De Caneva, G.; De Lotto, B.; de Oña Wilhelmi, E.; Delgado Mendez, C.; Di Pierro, F.; Dominis Prester, D.; Dorner, D.; Doro, M.; Einecke, S.; Eisenacher, D.; Elsaesser, D.; Fernández-Barral, A.; Fidalgo, D.; Fonseca, M. V.; Font, L.; Frantzen, K.; Fruck, C.; Galindo, D.; García López, R. J.; Garczarczyk, M.; Garrido Terrats, D.; Gaug, M.; Godinović, N.; González Muñoz, A.; Gozzini, S. R.; Hadasch, D.; Hanabata, Y.; Hayashida, M.; Herrera, J.; Hose, J.; Hrupec, D.; Idec, W.; Kadenius, V.; Kellermann, H.; Knoetig, M. L.; Kodani, K.; Konno, Y.; Krause, J.; Kubo, H.; Kushida, J.; La Barbera, A.; Lelas, D.; Lewandowska, N.; Lindfors, E.; Lombardi, S.; Longo, F.; López, M.; López-Coto, R.; López-Oramas, A.; Lorenz, E.; Lozano, I.; Makariev, M.; Mallot, K.; Maneva, G.; Mannheim, K.; Maraschi, L.; Marcote, B.; Mariotti, M.; Martínez, M.; Mazin, D.; Menzel, U.; Miranda, J. M.; Mirzoyan, R.; Moralejo, A.; Munar-Adrover, P.; Nakajima, D.; Neustroev, V.; Niedzwiecki, A.; Nievas Rosillo, M.; Nilsson, K.; Nishijima, K.; Noda, K.; Orito, R.; Overkemping, A.; Paiano, S.; Palatiello, M.; Paneque, D.; Paoletti, R.; Paredes, J. M.; Paredes-Fortuny, X.; Persic, M.; Poutanen, J.; Prada Moroni, P. G.; Prandini, E.; Puljak, I.; Reinthal, R.; Rhode, W.; Ribó, M.; Rico, J.; Rodriguez Garcia, J.; Saito, T.; Saito, K.; Satalecka, K.; Scalzotto, V.; Scapin, V.; Schultz, C.; Schweizer, T.; Shore, S. N.; Sillanpää, A.; Sitarek, J.; Snidaric, I.; Sobczynska, D.; Stamerra, A.; Steinbring, T.; Strzys, M.; Takalo, L.; Takami, H.; Tavecchio, F.; Temnikov, P.; Terzić, T.; Tescaro, D.; Teshima, M.; Thaele, J.; Torres, D. F.; Toyama, T.; Treves, A.; Vogler, P.; Will, M.; Zanin, R.; Berger, K.; Buson, S.; D'Ammando, F.; Gasparrini, D.; Hovatta, T.; Max-Moerbeck, W.; Readhead, A.; Richards, J.

    2015-07-01

    The high-frequency-peaked BL Lac (HBL) 1ES 0806+524 (z = 0.138) was discovered in very high energy (VHE) γ-rays in 2008. Until now, the broad-band spectrum of 1ES 0806+524 has been only poorly characterized, in particular at high energies. We analysed multiwavelength observations from γ-rays to radio performed from 2011 January to March, which were triggered by the high activity detected at optical frequencies. These observations constitute the most precise determination of the broad-band emission of 1ES 0806+524 to date. The stereoscopic Major Atmospheric Gamma-Ray Imaging Cherenkov (MAGIC) observations yielded a γ-ray signal above 250 GeV of (3.7 ± 0.7) per cent of the Crab Nebula flux with a statistical significance of 9.9σ. The multiwavelength observations showed significant variability in essentially all energy bands, including a VHE γ-ray flare that lasted less than one night, which provided unprecedented evidence for short-term variability in 1ES 0806+524. The spectrum of this flare is well described by a power law with a photon index of 2.97 ± 0.29 between ˜150 GeV and 1 TeV and an integral flux of (9.3 ± 1.9) per cent of the Crab nebula flux above 250 GeV. The spectrum during the non-flaring VHE activity is compatible with the only available VHE observation performed in 2008 with VERITAS when the source was in a low optical state. The broad-band spectral energy distribution can be described with a one-zone synchrotron self-Compton model with parameters typical for HBLs, indicating that 1ES 0806+524 is not substantially different from the HBLs previously detected.

  4. LSWS linked with the low-latitude Es and its implications for the growth of the R-T instability

    NASA Astrophysics Data System (ADS)

    Joshi, L. M.

    2016-07-01

    A comprehensive investigation of spread F irregularities over the Indian sector has been carried out using VHF radar and ionosonde observations. Two different categories of spread F observations, one where the onset of the range spread F (RSF) was concurrent with the peak h'F (category 1) and another where the RSF onset happened ~90 min after the peak h'F time (category 2), are presented. RSF in category 2 was preceded by the presence of oblique echoes in ionograms, indicating the irregularity genesis westward of Sriharikota. The average peak h'F in category 1 was ~30 km higher than that in category 2 indicating the presence of standing large-scale wave structure (LSWS). Occurrence of the blanketing Es during 19:30 to 20:30 Indian Standard Time in category 1 (category 2) was 0% (>50%). Model computation is also carried out to further substantiate the observational results. Model computation indicates that zonal variation of low-latitude Es can generate zonal modulation in the F layer height rise. It is found that the modulation of the F layer height, linked with the low-latitude Es, assists the equatorial spread F onset by modifying both the growth rate of the collisional Rayleigh-Taylor (R-T) instability and also its efficiency. A predominant presence of low-latitude Es has been observed, but the increase in the F layer height and the R-T instability growth in the evening hours will maximize with complete absence of low-latitude Es. A new mechanism for the generation of LSWS and its implications on R-T instability is discussed.

  5. Richland Operations (DOE-RL) Environmental Safety Health (ES and H) FY 2000 and FY 2001 Execution Commitment Summary

    SciTech Connect

    REEP, I.E.

    2000-12-01

    All sites in the U.S. Department of Energy (DOE) Complex prepare this report annually for the DOE Office of Environment, Safety and Health (EH). The purpose of this report is to provide a summary of the previous and current year's Environment, Safety and Health (ES&H) execution commitments and the Safety and Health (S&H) resources that support these activities. The fiscal year (FY) 2000 and 2001 information and data contained in the Richland Operations Environment, Safefy and Health Fiscal Year 2002 Budget-Risk Management Summary (RL 2000a) were the basis for preparing this report. Fiscal year 2001 activities are based on the President's Amended Congressional Budget Request of $689.6 million for funding Ofice of Environmental Management (EM) $44.0 million for Fast Flux Test Facility standby less $7.0 million in anticipated DOE, Headquarters holdbacks for Office of Nuclear Energy, Science and Technology (NE); and $55.3 million for Safeguards and Security (SAS). Any funding changes as a result of the Congressional appropriation process will be reflected in the Fiscal Year 2003 ES&H Budget-Risk Management Summary to be issued in May 2001. This report provides the end-of-year status of FY 2000 ES&H execution commitments, including actual S&H expenditures, and describes planned FY 2001 ES&H execution commitments and the S&H resources needed to support those activities. This requirement is included in the ES&H guidance contained in the FY 2002 Field Budget Call (DOE 2000).

  6. Purification and characterization of AsES protein: a subtilisin secreted by Acremonium strictum is a novel plant defense elicitor.

    PubMed

    Chalfoun, Nadia R; Grellet-Bournonville, Carlos F; Martínez-Zamora, Martín G; Díaz-Perales, Araceli; Castagnaro, Atilio P; Díaz-Ricci, Juan C

    2013-05-17

    In this work, the purification and characterization of an extracellular elicitor protein, designated AsES, produced by an avirulent isolate of the strawberry pathogen Acremonium strictum, are reported. The defense eliciting activity present in culture filtrates was recovered and purified by ultrafiltration (cutoff, 30 kDa), anionic exchange (Q-Sepharose, pH 7.5), and hydrophobic interaction (phenyl-Sepharose) chromatographies. Two-dimensional SDS-PAGE of the purified active fraction revealed a single spot of 34 kDa and pI 8.8. HPLC (C2/C18) and MS/MS analysis confirmed purification to homogeneity. Foliar spray with AsES provided a total systemic protection against anthracnose disease in strawberry, accompanied by the expression of defense-related genes (i.e. PR1 and Chi2-1). Accumulation of reactive oxygen species (e.g. H2O2 and O2(˙)) and callose was also observed in Arabidopsis. By using degenerate primers designed from the partial amino acid sequences and rapid amplification reactions of cDNA ends, the complete AsES-coding cDNA of 1167 nucleotides was obtained. The deduced amino acid sequence showed significant identity with fungal serine proteinases of the subtilisin family, indicating that AsES is synthesized as a larger precursor containing a 15-residue secretory signal peptide and a 90-residue peptidase inhibitor I9 domain in addition to the 283-residue mature protein. AsES exhibited proteolytic activity in vitro, and its resistance eliciting activity was eliminated when inhibited with PMSF, suggesting that its proteolytic activity is required to induce the defense response. This is, to our knowledge, the first report of a fungal subtilisin that shows eliciting activity in plants. This finding could contribute to develop disease biocontrol strategies in plants by activating its innate immunity. PMID:23530047

  7. Gender differences in diet and nutrition among adults initiating antiretroviral therapy in Dar es Salaam, Tanzania.

    PubMed

    Abioye, Ajibola I; Isanaka, Sheila; Liu, Enju; Mwiru, Ramadhani S; Noor, Ramadhani A; Spiegelman, Donna; Mugusi, Ferdinand; Fawzi, Wafaie

    2015-01-01

    Human immunodeficiency virus (HIV)-infected males have poor treatment outcomes after initiation of antiretroviral therapy (ART) compared to HIV-infected women. Dietary factors might mediate the association between sex and disease progression. However, the gender difference in diet among HIV-infected individuals in sub-Saharan Africa is largely unknown. The objective of this study was to examine differences in dietary intake among HIV-infected men and women. We conducted a cross-sectional analysis of dietary questionnaire data from 2038 adults initiating ART in Dar es Salaam, Tanzania to assess whether nutrient adequacy differed by sex. We dichotomized participants' nutrient intakes by whether recommended dietary allowances (RDAs) were met and estimated the relative risk (RR) of meeting RDAs in males using binomial regression models. We also estimated the mean difference in intake of foods and food groups by gender. We found poorer dietary practices among men compared to women. Males were less likely to meet the RDAs for micronutrients critical for slowing disease progression among HIV patients: niacin (RR = 0.39, 95% confidence interval [CI]: 0.27 to 0.55), riboflavin (RR = 0.81, 95% CI: 0.73 to 0.91), vitamin C (RR = 0.94, 95% CI: 0.89 to 1.00), and zinc (RR = 0.06, 95% CI: 0.01 to 0.24). Intake of thiamine, pantothenate, vitamins B6, B12, and E did not vary by gender. Males were less likely to eat cereals (mean difference [servings per day] = -0.21, 95% CI: -0.44 to 0.001) and vegetables (mean difference = -0.47, 95% CI: -0.86 to -0.07) in their diet, but more likely to have meat (mean difference = 0.14, 95% CI: 0.06 to 0.21). We conclude that male HIV patients have poorer dietary practices than females, and this may contribute to faster progression of the disease in males.

  8. Cool and warm dust emission from M 33 (HerM33es)

    NASA Astrophysics Data System (ADS)

    Xilouris, E. M.; Tabatabaei, F. S.; Boquien, M.; Kramer, C.; Buchbender, C.; Bertoldi, F.; Anderl, S.; Braine, J.; Verley, S.; Relaño, M.; Quintana-Lacaci, G.; Akras, S.; Beck, R.; Calzetti, D.; Combes, F.; Gonzalez, M.; Gratier, P.; Henkel, C.; Israel, F.; Koribalski, B.; Lord, S.; Mookerjea, B.; Rosolowsky, E.; Stacey, G.; Tilanus, R. P. J.; van der Tak, F.; van der Werf, P.

    2012-07-01

    In the framework of the open-time key program "Herschel M 33 extended survey (HerM33es)", we study the far-infrared emission from the nearby spiral galaxy M 33 in order to investigate the physical properties of the dust such as its temperature and luminosity density across the galaxy. Taking advantage of the unique wavelength coverage (100, 160, 250, 350, and 500 μm) of the Herschel Space Observatory and complementing our dataset with Spitzer-IRAC 5.8 and 8 μm and Spitzer-MIPS 24 and 70 μm data, we construct temperature and luminosity density maps by fitting two modified blackbodies of a fixed emissivity index of 1.5. We find that the "cool" dust grains are heated to temperatures of between 11 K and 28 K, with the lowest temperatures being found in the outskirts of the galaxy and the highest ones both at the center and in the bright HII regions. The infrared/submillimeter total luminosity (5-1000 μm) is estimated to be 1.9 × 109 -4.4×108+4.0×108L⊙. Fifty-nine percent of the total infrared/submillimeter luminosity of the galaxy is produced by the "cool" dust grains (~15 K), while the remaining 41% is produced by "warm" dust grains (~55 K). The ratio of the cool-to-warm dust luminosity is close to unity (within the computed uncertainties), throughout the galaxy, with the luminosity of the cool dust being slightly higher at the center than the outer parts of the galaxy. Decomposing the emission of the dust into two components (one emitted by the diffuse disk of the galaxy and one emitted by the spiral arms), we find that the fraction of the emission from the disk in the mid-infrared (24 μm) is 21%, while it gradually rises up to 57% in the submillimeter (500 μm). We find that the bulk of the luminosity comes from the spiral arm network that produces 70% of the total luminosity of the galaxy with the rest coming from the diffuse dust disk. The "cool" dust inside the disk is heated to temperatures in a narrow range between 18 K and 15 K (going from the center

  9. SCIDIP-ES - A science data e-infrastructure for preservation of earth science data

    NASA Astrophysics Data System (ADS)

    Riddick, Andrew; Glaves, Helen; Marelli, Fulvio; Albani, Mirko; Tona, Calogera; Marketakis, Yannis; Tzitzikas, Yannis; Guarino, Raffaele; Giaretta, David; Di Giammatteo, Ugo

    2013-04-01

    The capability for long term preservation of earth science data is a key requirement to support on-going research and collaboration within and between many earth science disciplines. A number of critically important current research directions (e.g. understanding climate change, and ensuring sustainability of natural resources) rely on the preservation of data often collected over several decades in a form in which it can be accessed and used easily. In many branches of the earth sciences the capture of key observational data may be difficult or impossible to repeat. For example, a specific geological exposure or subsurface borehole may be only temporarily available, and deriving earth observation data from a particular satellite mission is clearly often a unique opportunity. At the same time such unrepeatable observations may be a critical input to environmental, economic and political decision making. Another key driver for strategic long term data preservation is that key research challenges (such as those described above) frequently require cross disciplinary research utilising raw and interpreted data from a number of earth science disciplines. Effective data preservation strategies can support this requirement for interoperability, and thereby stimulate scientific innovation. The SCIDIP-ES project (EC FP7 grant agreement no. 283401) seeks to address these and other data preservation challenges by developing a Europe wide e-infrastructure for long term data preservation comprising appropriate software tools and infrastructure services to enable and promote long term preservation of earth science data. Because we define preservation in terms of continued usability of the digitally encoded information, the generic infrastructure services will allow a wide variety of data to be made usable by researchers from many different domains. This approach will enable the cost for long-term usability across disciplines to be shared supporting the creation of strong

  10. Teaching a new dog old tricks: the synergy of ISO 14000, NEPA, and integrated ES{ampersand}H management

    SciTech Connect

    Wilkinson, C.H.

    1997-03-01

    For more than twenty-five years, federal agencies have wrestled with (and even learned from) the planning and decision making processes of the National Environmental Policy Act (NEPA). Accordingly, agencies have developed established processes for environmental planning, impact assessment,and environmental-based decision making. Agencies are now faced with an opportunity to align existing environmental planning systems developed under NEPA with those of ISO 14001, the new international standard for environmental management systems. Through experience gained with NEPA, agencies may have an opportunity to assist the private sector through sharing of lessons learned in identification and mitigation of environmental aspects and impacts. However, agencies should also learn from the private sector how integrated environmental management includes integrating environment, safety, and health (ES&H) considerations in such away as to add direct value to the business. In times of continued and increasing federal agency downsizing, the government can streamline ES&H management planning by integrating ES&H values with business goals. The first synergy of NEPA and ISO 14001 is the identification and assessment of environmental impacts. Under IS0 14001,an organization must identify the `environmental aspects of its activities, products or services`. This is similar to the approach taken in NEPA where agencies must evaluate significant environmental impacts of its actions. The second synergy is the reduction and mitigation of the impacts. IS0 14001 requires a commitment to prevention of pollution and the NEPA process integrates pollution prevention with environmental planning. IS0 14001 requires checking and corrective action to monitor and measure progress toward environmental goals. NEPA applies mitigation measures to avoid or mitigate potential impacts. Because agencies have been conducting NEPA impact assessment for more than twenty-five years, this body of impact assessment

  11. Enhanced expression of EsWAX1 improves drought tolerance with increased accumulation of cuticular wax and ascorbic acid in transgenic Arabidopsis.

    PubMed

    Zhu, Lin; Guo, Jiansheng; Zhu, Jian; Zhou, Cheng

    2014-02-01

    Drought can activate several stress responses in plants, such as stomatal closure, accumulation of cuticular wax and ascorbic acid (AsA), which have been correlated with improvement of drought tolerance. In this study, a novel MYB gene, designed as EsWAX1, was isolated and characterized from Eutrema salsugineum. EsWAX1 contained a full-length open reading frame (ORF) of 1068 bp, which encoding 355 amino acids. Transcript levels of EsWAX1 were quickly inducible by drought stress and ABA treatment, indicating that EsWAX1 may act as a positive regulator in response to drought stress. Ectopic expression of EsWAX1 increased accumulation of cuticular wax via modulating the expression of several wax-related genes, such as CER1, KCS2 and KCR1. Scanning electron microscopy further revealed higher densities of wax crystalline structures on the adaxial surfaces of leaves in transgenic Arabidopsis plants. In addition, the expression of several AsA biosynthetic genes (VTC1, GLDH and MIOX4) was significantly up-regulated in EsWAX1-overexpressing lines and these transgenic plants have approximately 23-27% more total AsA content than WT plants. However, the high-level expression of EsWAX1 severely disrupted plant normal growth and development. To reduce negative effects of EsWAX1 over-expression on plant growth, we generated transgenic Arabidopsis plants expressing EsWAX1 driven by the stress-inducible RD29A promoter. Our data indicated the RD29A::EsWAX1 transgenic plants had greater tolerance to drought stress than wild-type plants. Taken together, the EsWAX1 gene is a potential regulator that may be utilized to improve plant drought tolerance by genetic manipulation.

  12. Inference of gene regulation via miRNAs during ES cell differentiation using MiRaGE method.

    PubMed

    Yoshizawa, Masato; Taguchi, Y-H; Yasuda, Jun

    2011-01-01

    MicroRNA (miRNA) is a critical regulator of cell growth, differentiation, and development. To identify important miRNAs in a biological process, many bioinformatical tools have been developed. We have developed MiRaGE (MiRNA Ranking by Gene Expression) method to infer the regulation of gene expression by miRNAs from changes of gene expression profiles. The method does not require precedent array normalization. We applied the method to elucidate possibly important miRNAs during embryonic stem (ES) cell differentiation to neuronal cells and we infer that certain miRNAs, including miR-200 family, miR-429, miR-302 family, and miR-17-92 cluster members may be important to the maintenance of undifferentiated status in ES cells. PMID:22272132

  13. The development and application of k0-standardization method of neutron activation analysis at Es-Salam research reactor

    NASA Astrophysics Data System (ADS)

    Alghem, L.; Ramdhane, M.; Khaled, S.; Akhal, T.

    2006-01-01

    In recent years the k0-NAA method has been applied and developed at the 15 MW Es-Salam research reactor, which includes: (1) the detection efficiency calibration of γ-spectrometer used in k0-NAA, (2) the determination of reactor neutron spectrum parameters such as α and f factors in the irradiation channel, and (3) the validation of the developed k0-NAA procedure by analysing SRM, namely AIEA-Soil7 and CRM, namely IGGE-GSV4. The analysis results obtained by k0-NAA with 27 elements of Soil-7 standard and 14 elements of GSV-4 standard were compared with certified values. The analysis results showed that the deviations between experimental and certified values were mostly less than 10%. The k0-NAA procedure established at Es-Salam research reactor has been regarded as a reliable standardization method of NAA and as available for practical applications.

  14. Evolution of blue E/S0 galaxies from z ~ 1: merger remnants or disk-rebuilding galaxies?

    NASA Astrophysics Data System (ADS)

    Huertas-Company, M.; Aguerri, J. A. L.; Tresse, L.; Bolzonella, M.; Koekemoer, A. M.; Maier, C.

    2010-06-01

    Context. Studying outliers from the bimodal distribution of galaxies in the color-mass space, such as morphological early-type galaxies residing in the blue cloud (blue E/S0s), can help for better understanding the physical mechanisms that lead galaxy migrations in this space. Aims: In this paper we try to bring new clues to studying the evolution of the properties of a significant sample of blue E/S0 galaxies in the COSMOS field. Methods: We define blue E/S0 galaxies as objects having a clear early-type morphology on the HST/ACS images (according to our automated classification scheme galSVM) but with a blue rest-frame color (defined by using the SED best-fit template on the COSMOS primary photometric catalogs). Combining these two measurements with spectroscopic redshifts from the zCOSMOS 10k release, we isolated 210 IAB < 22 blue early-type galaxies with M_*/M_⊙ > 1010 in three redshift bins (0.2 < z < 0.55, 0.55 < z < 0.8, 0.8 < z < 1.4) and studied the evolution of their properties (number density, SFR, morphology, size). Results: The threshold mass (Mt), defined at z = 0 in previous studies as the mass below which the population of blue early-type galaxies starts to be abundant relative to passive E/S0s, evolves from log (M_*/M_⊙) ~ 10.1 ± 0.35 at z ~ 0.3 to log (M_*/M_⊙) ~ 10.9 ± 0.35 at z ~ 1. Interestingly, it follows the evolution of the crossover mass between the early and late type populations (bimodality mass) indicating that the abundance of blue E/S0 is another measure of the downsizing effect in the build-up of the red sequence. There seems to be a turn-over mass in the nature of blue E/S0 galaxies. Above log (M_*/M_⊙) ~ 10.8 blue E/S0 resemble to merger remnants probably migrating to the red sequence on a time scale of ˜3 Gyr. Below this mass, they seem to be closer to normal late-type galaxies, as if they were the result of minor mergers that triggered the central star formation and built a central bulge component or were (re)building a

  15. Journées 2013 "Systèmes de Référence Spatio-Temporels"

    NASA Astrophysics Data System (ADS)

    Capitaine, Nicole

    2014-12-01

    The Journées 2013 "Systèmes de référence spatio-temporels", with the sub-title "Scientific developments from highly accurate space-time reference systems", were organized from 16 to 18 September 2013 at Paris Observatory and Ecole Normale Supérieure in Paris, France. The scientific programme was composed of the following sessions: S1: Theoretical aspects of reference systems; S2: The next ICRF - Progress and developments; S3: Atomic and pulsar-based time scales - Progress and developments; S4a: Earth rotation - Theory; S4b: Earth rotation - Modelling and observations; S5: Solar system dynamics - Theory, modelling and numerical standards. In addition, there have been presentations and discussions related to the new IAU Division A Working Groups that have been established at the 28th IAU GA in 2012, with WG meetings organized in association with the Journées.

  16. An ES cell system for rapid, spatial and temporal analysis of gene function in vitro and in vivo

    PubMed Central

    Mao, Junhao; Barrow, Jeffery; McMahon, Jill; Vaughan, Joe; McMahon, Andrew P.

    2005-01-01

    We describe a versatile genetic system for rapid analysis of mammalian gene function. In this, loss of reporter activity in a novel embryonic stem (ES) cell line enables rapid identification of targeting to the ubiquitously expressed Rosa26 locus. Subsequent regulation of gene activity is governed by a dual regulatory strategy utilizing two drugs, Tamoxifen and Doxycycline. To illustrate this approach, a dominant allele of Smoothened was introduced into this cell line, enabling regulated activation of Hedgehog signaling. By coupling Cre-loxP dependent activation with tetracycline dependent transcription in a single allele, we established a conditional method to control Smoothened activity and neural progenitor specification in differentiating ES cells in vitro and in chimeric embryos in vivo When crossed to an appropriate Cre driver strain, gene activity can also be temporally regulated within a specific cell lineage. This platform will facilitate rapid analysis of gene function in the mouse. PMID:16221970

  17. Quantitative morphology of E-S0 galaxies IV. Ellipticals and lenticulars as a single population

    NASA Astrophysics Data System (ADS)

    Michard, R.

    1994-08-01

    it can be estimated without detailed modeling, against the maximum ellipticity. The "standard" structure of E-S0 objects includes a spheroidal, nearly oblate component, that cannot be of constant ellipticity: this parameter often increases outwards from its bulge value, then decreases again in the envelope. Disks of various brightness and extent, between dominating and vanishing, are the second essential component. Obviously, their relative importance is, together with the dust content, a physical criterion involved in the morphological separation between E and S0 objects, besides the inclination effect. In the present sample, the disk cannot be detected in nearly half of E classified objects, and is probably vanishing in about 40% of this class, or circa 17% of the total of studied early-type objects. This minority contains mostly boxy E's with non-standard ellipticity profiles, and, as is well known, no significant rotational support. From the convergent results of quantitative morphology and kinematics, the early-type galaxies should be segregated into the majority class of disky fast oblate rotators and a minority class of non-rotating ellipsoids.

  18. DNA stable pentaploid H1 (ES) cells obtained from an octaploid cell induced from tetraploid cells polyploidized using demecolcine.

    PubMed

    Fujikawa-Yamamoto, Kohzaburo; Luo, Xianwen; Miyagoshi, Minoru; Yamagishi, Hiroko

    2010-05-01

    Pentaploid H1 (ES) cells (5H1 cells) were accidentally obtained through one-cell cloning of octaploid H1 (ES) cells (8H1 cells) that were established from tetraploid H1 (ES) cells (4H1 cells) polyploidized using demecolcine. The number of chromosomes of 5H1 cells was 100, unlike the 40 of diploid H1 (ES) cells (2H1 cells), 80 of 4H1, and 160 of 8H1 cells. The durations of G(1), S, and G(2)/M phases of 5H1 cells were 3, 7, and 6 h, respectively, almost the same as those of 2H1, 4H1, and 8H1 cells. The cell volume of 5H1 cells was half of that of 8H1 cells, suggesting that 5H1 cells were created through abnormal cell divisions of 8H1 cells. The morphology of growing 5H1 cells was a spherical cluster similar to that of 2H1 cells and differing from the flagstone-like shape of 4H1 and 8H1 cells. Pentaploid solid tumors were formed from 5H1 cells after interperitoneal injection into the mouse abdomen, and they contained endodermal, mesodermal, and ectodermal cells as well as undifferentiated cells, suggesting both that the DNA content of 5H1 cells was retained during tumor formation and that the 5H1 cells were pluripotent. The DNA content of 5H1 cells was stable in long-term culturing as 2H1 cells, meaning that 5H1 and 2H1 cells shared similarities in DNA structure. The excellent stability of the DNA content of 5H1 cells was explained using a hypothesis for the DNA structure of polyploid cells because the pairing of homologous chromosomes in 5H1 cells is spatially forbidden. PMID:20082301

  19. Neutrino Emission in the Hadronic Synchrotron Mirror Model: The "Orphan" Flare from 1ES 1959+650

    NASA Technical Reports Server (NTRS)

    Reimer, A.; Boettcher, M.; Postnikov, S.

    2005-01-01

    A challenge to standard leptonic synchrotron self-Compton (SSC) models is the so-called orphan TeV flares, i.e., enhanced very high energy (VHE) gamma-ray emission without any contemporaneous X-ray flaring activity, that have recently been observed in TeV blazars (e.g., 1ES 1959+650). In order to explain the orphan TeV flare of 1 ES 1959+650 observed in 2002 June, the so-called hadronic synchrotron mirror model has been developed. Here relativistic protons are proposed to exist in the jet and interact with reflected electron synchrotron radiation of the precursor SSC flare. If the reflector is located in the cloud region, time shifts of several days are possible between the precursor and the orphan flare. The external photons, blue-shifted in the comoving jet frame, are able to excite the Delta(1232) resonance when interacting with protons of Lorentz factors gamma(sub p) approx. 10(exp 3)-10(exp 4). The decay products of this resonance include charged pions, which, on decay, give rise to neutrino production during the orphan flare. In this paper we calculate the expected neutrino emission for the 2002 June 4 orphan TeV flare of 1ES 1959+650. We compare our results with the recent observations of AMANDA-II of a neutrino event in spatial and temporal coincidence with the orphan flare of this blazar. We find that the expected neutrino signal from the hadronic synchrotron mirror model is insufficient to explain the claimed neutrino signal from the direction of 1ES 1959+650.

  20. Can manipulation of differentiation conditions eliminate proliferative cells from a population of ES cell-derived forebrain cells?

    PubMed

    Precious, Sophie V; Kelly, Claire M; Allen, Nicholas D; Rosser, Anne E

    2016-01-01

    There is preliminary evidence that implantation of primary fetal striatal cells provides functional benefit in patients with Huntington's disease, a neurodegenerative condition resulting in loss of medium-sized spiny neurons (MSN) of the striatum. Scarcity of primary fetal tissue means it is important to identify a renewable source of cells from which to derive donor MSNs. Embryonic stem (ES) cells, which predominantly default to telencephalic-like precursors in chemically defined medium (CDM), offer a potentially inexhaustible supply of cells capable of generating the desired neurons. Using an ES cell line, with the forebrain marker FoxG1 tagged to the LacZ reporter, we assessed effects of known developmental factors on the yield of forebrain-like precursor cells in CDM suspension culture. Addition of FGF2, but not DKK1, increased the proportion of FoxG1-expressing cells at day 8 of neural induction. Oct4 was expressed at day 8, but was undetectable by day 16. Differentiation of day 16 precursors generated GABA-expressing neurons, with few DARPP32 positive MSNs. Transplantation of day 8 precursor cells into quinolinic acid-lesioned striata resulted in generation of teratomas. However, transplantation of day 16 precursors yielded grafts expressing neuronal markers including NeuN, calbindin and parvalbumin, but no DARPP32 6 weeks post-transplantation. Manipulation of fate of ES cells requires optimization of both concentration and timing of addition of factors to culture systems to generate the desired phenotypes. Furthermore, we highlight the value of increasing the precursor phase of ES cell suspension culture when directing differentiation toward forebrain fate, so as to dramatically reduce the risk of teratoma formation. PMID:27606335

  1. HESS and Fermi-LAT discovery of γ-rays from the blazar 1ES 1312-423

    NASA Astrophysics Data System (ADS)

    HESS Collaboration; Abramowski, A.; Acero, F.; Aharonian, F.; Akhperjanian, A. G.; Angüner, E.; Anton, G.; Balenderan, S.; Balzer, A.; Barnacka, A.; Becherini, Y.; Becker Tjus, J.; Bernlöhr, K.; Birsin, E.; Bissaldi, E.; Biteau, J.; Boisson, C.; Bolmont, J.; Bordas, P.; Brucker, J.; Brun, F.; Brun, P.; Bulik, T.; Carrigan, S.; Casanova, S.; Cerruti, M.; Chadwick, P. M.; Chalme-Calvet, R.; Chaves, R. C. G.; Cheesebrough, A.; Chrétien, M.; Colafrancesco, S.; Cologna, G.; Conrad, J.; Couturier, C.; Dalton, M.; Daniel, M. K.; Davids, I. D.; Degrange, B.; Deil, C.; deWilt, P.; Dickinson, H. J.; Djannati-Ataï, A.; Domainko, W.; Drury, L. O'C.; Dubus, G.; Dutson, K.; Dyks, J.; Dyrda, M.; Edwards, T.; Egberts, K.; Eger, P.; Espigat, P.; Farnier, C.; Fegan, S.; Feinstein, F.; Fernandes, M. V.; Fernandez, D.; Fiasson, A.; Fontaine, G.; Förster, A.; Füßling, M.; Gajdus, M.; Gallant, Y. A.; Garrigoux, T.; Gast, H.; Giebels, B.; Glicenstein, J. F.; Göring, D.; Grondin, M.-H.; Grudzińska, M.; Häffner, S.; Hague, J. D.; Hahn, J.; Harris, J.; Heinzelmann, G.; Henri, G.; Hermann, G.; Hervet, O.; Hillert, A.; Hinton, J. A.; Hofmann, W.; Hofverberg, P.; Holler, M.; Horns, D.; Jacholkowska, A.; Jahn, C.; Jamrozy, M.; Janiak, M.; Jankowsky, F.; Jung, I.; Kastendieck, M. A.; Katarzyński, K.; Katz, U.; Kaufmann, S.; Khélifi, B.; Kieffer, M.; Klepser, S.; Klochkov, D.; Kluźniak, W.; Kneiske, T.; Kolitzus, D.; Komin, Nu.; Kosack, K.; Krakau, S.; Krayzel, F.; Krüger, P. P.; Laffon, H.; Lamanna, G.; Lefaucheur, J.; Lemoine-Goumard, M.; Lenain, J.-P.; Lennarz, D.; Lohse, T.; Lopatin, A.; Lu, C.-C.; Marandon, V.; Marcowith, A.; Maurin, G.; Maxted, N.; Mayer, M.; McComb, T. J. L.; Medina, M. C.; Méhault, J.; Menzler, U.; Meyer, M.; Moderski, R.; Mohamed, M.; Moulin, E.; Murach, T.; Naumann, C. L.; de Naurois, M.; Nedbal, D.; Niemiec, J.; Nolan, S. J.; Oakes, L.; Ohm, S.; de Oña Wilhelmi, E.; Opitz, B.; Ostrowski, M.; Oya, I.; Panter, M.; Parsons, R. D.; Paz Arribas, M.; Pekeur, N. W.; Pelletier, G.; Perez, J.; Petrucci, P.-O.; Peyaud, B.; Pita, S.; Poon, H.; Pühlhofer, G.; Punch, M.; Quirrenbach, A.; Raab, S.; Raue, M.; Reimer, A.; Reimer, O.; Renaud, M.; de los Reyes, R.; Rieger, F.; Rob, L.; Rosier-Lees, S.; Rowell, G.; Rudak, B.; Rulten, C. B.; Sahakian, V.; Sanchez, D. A.; Santangelo, A.; Schlickeiser, R.; Schüssler, F.; Schulz, A.; Schwanke, U.; Schwarzburg, S.; Schwemmer, S.; Sol, H.; Spengler, G.; Spieß, F.; Stawarz, Ł.; Steenkamp, R.; Stegmann, C.; Stinzing, F.; Stycz, K.; Sushch, I.; Szostek, A.; Tavernet, J.-P.; Terrier, R.; Tluczykont, M.; Trichard, C.; Valerius, K.; van Eldik, C.; Vasileiadis, G.; Venter, C.; Viana, A.; Vincent, P.; Völk, H. J.; Volpe, F.; Vorster, M.; Wagner, S. J.; Wagner, P.; Ward, M.; Weidinger, M.; White, R.; Wierzcholska, A.; Willmann, P.; Wörnlein, A.; Wouters, D.; Zacharias, M.; Zajczyk, A.; Zdziarski, A. A.; Zech, A.; Zechlin, H.-S.; Perkins, J. S.; Ojha, R.; Stevens, J.; Edwards, P. G.; Kadler, M.

    2013-09-01

    A deep observation campaign carried out by the High Energy Stereoscopic System (HESS) on Centaurus A enabled the discovery of γ-rays from the blazar 1ES 1312-423, 2° away from the radio galaxy. With a differential flux at 1 TeV of φ(1 TeV) = (1.9 ± 0.6stat ± 0.4sys) × 10-13 cm-2 s-1 TeV-1 corresponding to 0.5 per cent of the Crab nebula differential flux and a spectral index Γ = 2.9 ± 0.5stat ± 0.2sys, 1ES 1312-423 is one of the faintest sources ever detected in the very high energy (E > 100 GeV) extragalactic sky. A careful analysis using three and a half years of Fermi Large Area Telescope (Fermi-LAT) data allows the discovery at high energies (E > 100 MeV) of a hard spectrum (Γ = 1.4 ± 0.4stat ± 0.2sys) source coincident with 1ES 1312-423. Radio, optical, UV and X-ray observations complete the spectral energy distribution of this blazar, now covering 16 decades in energy. The emission is successfully fitted with a synchrotron self-Compton model for the non-thermal component, combined with a blackbody spectrum for the optical emission from the host galaxy.

  2. beta. -delayed fission from /sup 256/Es/sup m/ and the level scheme of /sup 256/Fm

    SciTech Connect

    Hall, H.L.; Gregorich, K.E.; Henderson, R.A.; Lee, D.M.; Hoffman, D.C.; Bunker, M.E.; Fowler, M.M.; Lysaght, P.; Starner, J.W.; Wilhelmy, J.B.; and others

    1989-05-01

    The 7.6-h isotope /sup 256/Es/sup m/ was produced from a 2.5-..mu..g/cm/sup 2/ target of /sup 254/Es by the (t,p) reaction. The reaction products were separated radiochemically, and the decay properties of /sup 256/Es/sup m/ were determined via ..beta..-..gamma.., ..gamma..-..gamma.., and ..beta..-fission correlation techniques. From these measurements we were able to assign 57 ..gamma.. rays to 26 levels in the daughter /sup 256/Fm. An isomeric level was observed at 1425 keV and assigned a spin and parity of 7/sup -/. This level has a t/sub 1/2/ of (70 +- 5) ns and we observed two ..beta..-delayed fissions with delay times in the proper time range to be associated with fission from this level. This gives a ..beta..-delayed fission probability of 2 x 10/sup -5/ for this level and a partial fission half-life of 0.8/sub -0.7//sup +8.8/ ms at the 95% confidence level.

  3. Roles for Histone Acetylation in Regulation of Telomere Elongation and Two-cell State in Mouse ES Cells.

    PubMed

    Dan, Jiameng; Yang, Jiao; Liu, Yifei; Xiao, Andrew; Liu, Lin

    2015-10-01

    Mammalian telomeres and subtelomeres are marked by heterochromatic epigenetic modifications, including repressive DNA methylation and histone methylation (e.g., H3K9me3 and H4K20me3). Loss of these epigenetic marks results in increased rates of telomere recombination and elongation. Other than these repressive epigenetic marks, telomeric and subtelomeric H3 and H4 are underacetylated. Yet, whether histone acetylation also regulates telomere length has not been directly addressed. We thought to test the effects of histone acetylation levels on telomere length using histone deacetylase (HDAC) inhibitor (sodium butyrate, NaB) that mediates histone hyperacetylation and histone acetyltransferase (HAT) inhibitor (C646) that mediates histone hypoacetylation. We show that histone hyperacetylation dramatically elongates telomeres in wild-type ES cells, and only slightly elongates telomeres in Terc(-/-) ES cells, suggesting that Terc is involved in histone acetylation-induced telomere elongation. In contrast, histone hypoacetylation shortens telomeres in both wild-type and Terc(-/-) ES cells. Additionally, histone hyperacetylation activates 2-cell (2C) specific genes including Zscan4, which is involved in telomere recombination and elongation, whereas histone hypoacetylation represses Zscan4 and 2C genes. These data suggest that histone acetylation levels affect the heterochromatic state at telomeres and subtelomeres, and regulate gene expression at subtelomeres, linking histone acetylation to telomere length maintenance.

  4. Klf6/copeb is required for hepatic outgrowth in zebrafish and for hepatocyte specification in mouse ES cells

    PubMed Central

    Zhao, Xiao; Monson, Christopher; Gao, Chuan; Gouon-Evans, Valerie; Matsumoto, Nobuyuki; Sadler, Kirsten C.; Friedman, Scott L

    2010-01-01

    Krüppel-like factor 6 (Klf6; copeb in zebrafish) is a zinc-finger transcription factor and tumor suppressor gene. Klf6−/− mice have defects in hematopoiesis and angiogenesis and do not form a liver. However, the vascular abnormalities in Klf6−/− mice obfuscate its role in liver development since these two processes are linked in mammals. We utilized zebrafish and mouse ES cells to investigate the role of copeb in endoderm specification and hepatogenesis separate from its function in angiogenesis. During zebrafish development, copeb expression is enriched in digestive organs. Morpholino knockdown of copeb blocks expansion of the liver, pancreas and intestine, but does not affect their specification, differentiation or the vascularization of the liver. Decreased hepatocyte proliferation in copeb morphants is accompanied by upregulation of the cell cycle inhibitor, cdkn1a, a Copeb transcriptional target. A cell autonomous role for Klf6 in endoderm and hepatic development was investigated by manipulating Klf6 expression in mouse ES cells driven to differentiate along the hepatic lineage. Expression of the endoderm markers Hnf3β, Gata4, Sox17, and CxCr4 is not induced in Klf6−/− cells but is upregulated in ES cells over-expressing Klf6. Collectively, these findings indicate that copeb/Klf6 is essential for the development of endoderm-derived organs. PMID:20430021

  5. Protective effect of small molecule analogues of the Acanthocheilonema viteae secreted product ES-62 on oxazolone-induced ear inflammation

    PubMed Central

    Al-Riyami, Lamyaa; Rodgers, David T.; Rzepecka, Justyna; Pineda, Miguel A.; Suckling, Colin J.; Harnett, Margaret M.; Harnett, William

    2015-01-01

    ES-62 is the major secreted protein of the rodent filarial nematode Acanthocheilonema viteae. The molecule contains covalently attached phosphorylcholine (PC) residues, which confer anti-inflammatory properties on ES-62, underpinning the idea that drugs based on this active moiety may have therapeutic potential in human diseases associated with aberrant inflammation. Here we demonstrate that two synthetic small molecule analogues (SMAs) of ES-62 termed SMA 11a and SMA 12b are protective in the oxazolone-induced acute allergic contact dermatitis mouse model of skin inflammation, as measured by a significant reduction in ear inflammation following their administration before oxazolone sensitisation and before oxazolone challenge. Furthermore, it was found that when tested, 12b was effective at reducing ear swelling even when first administered before challenge. Histological analysis of the ears showed elevated cellular infiltration and collagen deposition in oxazolone-treated mice both of which were reduced by treatment with the two SMAs. Likewise, the oxazolone-induced increase in IFNγ mRNA in the ears was reduced but no effect on other cytokines investigated was observed. Finally, no influence on the mast cell populations in the ear was observed. PMID:25836375

  6. nES GEMMA Analysis of Lectins and Their Interactions with Glycoproteins - Separation, Detection, and Sampling of Noncovalent Biospecific Complexes

    NASA Astrophysics Data System (ADS)

    Engel, Nicole Y.; Weiss, Victor U.; Marchetti-Deschmann, Martina; Allmaier, Günter

    2016-09-01

    In order to better understand biological events, lectin-glycoprotein interactions are of interest. The possibility to gather more information than the mere positive or negative response for interactions brought mass spectrometry into the center of many research fields. The presented work shows the potential of a nano-electrospray gas-phase electrophoretic mobility molecular analyzer (nES GEMMA) to detect weak, noncovalent, biospecific interactions besides still unbound glycoproteins and unreacted lectins without prior liquid phase separation. First results for Sambucus nigra agglutinin, concanavalin A, and wheat germ agglutinin and their retained noncovalent interactions with glycoproteins in the gas phase are presented. Electrophoretic mobility diameters (EMDs) were obtained by nES GEMMA for all interaction partners correlating very well with molecular masses determined by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of the individual molecules. Moreover, EMDs measured for the lectin-glycoprotein complexes were in good accordance with theoretically calculated mass values. Special focus was laid on complex formation for different lectin concentrations and binding specificities to evaluate the method with respect to results obtained in the liquid phase. The latter was addressed by capillary electrophoresis on-a-chip (CE-on-a-chip). Of exceptional interest was the fact that the formed complexes could be sampled according to their size onto nitrocellulose membranes after gas-phase separation. Subsequent immunological investigation further proved that the collected complex actually retained its native structure throughout nES GEMMA analysis and sampling.

  7. Proliferation assay of mouse embryonic stem (ES) cells exposed to atmospheric-pressure plasmas at room temperature

    NASA Astrophysics Data System (ADS)

    Miura, Taichi; Ando, Ayumi; Hirano, Kazumi; Ogura, Chika; Kanazawa, Tatsuya; Ikeguchi, Masamichi; Seki, Atsushi; Nishihara, Shoko; Hamaguchi, Satoshi

    2014-11-01

    Proliferation assays of mouse embryonic stem (ES) cells have been performed with cell culture media exposed to atmospheric-pressure plasmas (APPs), which generate reactive species in the media at room temperature. It is found that serum in cell culture media functions as a scavenger of highly reactive species and tends to protect cells in the media against cellular damage. On the other hand, if serum is not present in a cell culture medium when it is exposed to APP, the medium becomes cytotoxic and cannot be detoxified by serum added afterwards. Plasma-induced cytotoxic media hinder proliferation of mouse ES cells and may even cause cell death. It is also shown by nuclear magnetic resonance spectroscopy that organic compounds in cell culture media are in general not significantly modified by plasma exposure. These results indicate that if there is no serum in media when they are exposed to APPs, highly reactive species (such as OH radicals) generated in the media by the APP exposure are immediately converted to less reactive species (such as H2O2), which can no longer readily react with serum that is added to the medium after plasma exposure. This study has clearly shown that it is these less reactive species, rather than highly reactive species, that make the medium cytotoxic to mouse ES cells.

  8. Biofidelity evaluation of WorldSID and ES-2re under side impact conditions with and without airbag.

    PubMed

    Kim, Taewung; Shaw, Greg; Lessley, David; Park, Gwansik; Crandall, Jeff; Svendsen, Andy; Whitcomb, Bryant; Ayyagari, Murthy; Mishra, Prashast; Markusic, Craig

    2016-05-01

    This study evaluated the biofidelity of the WorldSID and the ES-2re under whole-body side impact conditions with and without a side airbag using the biomechanical cadaveric response data generated from 4.3m/s whole-body side impact tests. Impact forces, spinal kinematics, and chest deflections were considered in the biofidelity evaluation. Average responses and response corridors of PMHS were created using a time-alignment technique to reduce variability of the PMHS responses while maintaining the sum of the time shifts to be zero for each response. Biofidelity of the two dummies was compared using a correlation and analysis (CORA) method. The WorldSID demonstrated better biofidelity than the ES-2re in terms of CORA ratings in the conditions with airbag (0.53 vs. 0.46) and without an airbag (0.57 vs. 0.49). Lastly, the kinematic analysis of the two dummies indicated an overly compliant shoulder response of the WorldSID and excessive forward rotation of the ES-2re relative to the PMHS. PMID:26943014

  9. Analysis of variable VHE gamma-ray emission from the hard spectrum blazar 1ES 1218+304

    NASA Astrophysics Data System (ADS)

    Imran, Asif

    This thesis is a study of the very high energy gamma-ray emission from the hard spectrum blazar 1ES 1218+304. The data were collected during the 2008/09 observing season by the VERITAS observatory, an array of four atmospheric Cherenkov telescopes in Southern Arizona. This work describes the development of a set of analysis tools suitable for the extraction of the energy spectra of astrophysical objects. Initially, the tools are applied to the Crab nebula data to optimize and calibrate the analysis. Afterwards, the analysis is applied to the high energy observations of the blazar 1ES 1218+304. We report an intense, day-scale flare observed on January 30, 2009. This marks the first detection of variability in gamma-ray emission from 1ES 1218+304. I also investigate the possibility of detecting a spectral feature in the observed energy spectra of blazar due to extragalactic background light. I demonstrate the presence of a spectral cut-off in the simulated multi-TeV energy spectra of blazars at around 1 TeV. This novel technique has a strong potential to discover the first observable signature of absorption of very high-energy photons due to the extragalactic background light.

  10. SETDB1 modulates PRC2 activity at developmental genes independently of H3K9 trimethylation in mouse ES cells.

    PubMed

    Fei, Qi; Yang, Xiaoqin; Jiang, Hua; Wang, Qian; Yu, Yanyan; Yu, Yiling; Yi, Wei; Zhou, Shaolian; Chen, Taiping; Lu, Chris; Atadja, Peter; Liu, Xiaole Shirley; Li, En; Zhang, Yong; Shou, Jianyong

    2015-09-01

    SETDB1, a histone methyltransferase responsible for methylation of histone H3 lysine 9 (H3K9), is involved in maintenance of embryonic stem (ES) cells and early embryonic development of the mouse. However, how SETDB1 regulates gene expression during development is largely unknown. Here, we characterized genome-wide SETDB1 binding and H3K9 trimethylation (H3K9me3) profiles in mouse ES cells and uncovered two distinct classes of SETDB1 binding sites, termed solo and ensemble peaks. The solo peaks were devoid of H3K9me3 and enriched near developmental regulators while the ensemble peaks were associated with H3K9me3. A subset of the SETDB1 solo peaks, particularly those near neural development-related genes, was found to be associated with Polycomb Repressive Complex 2 (PRC2) as well as PRC2-interacting proteins JARID2 and MTF2. Genetic deletion of Setdb1 reduced EZH2 binding as well as histone 3 lysine 27 (H3K27) trimethylation level at SETDB1 solo peaks and facilitated neural differentiation. Furthermore, we found that H3K27me3 inhibits SETDB1 methyltransferase activity. The currently identified reciprocal action between SETDB1 and PRC2 reveals a novel mechanism underlying ES cell pluripotency and differentiation regulation.

  11. Using EC and ES cell culture to study early development: recent observations on Indian hedgehog and Bmps.

    PubMed

    Grabel, L; Becker, S; Lock, L; Maye, P; Zanders, T

    1998-01-01

    Despite great technological advances in the study of mammalian development in the past two decades, certain problems in early development, such as how the extraembryonic lineages are established, have remained intractable. We suggest that teratocarcinoma (EC) and embryonic stem cells (ES) remain useful in vitro tools for studying some of these problems. We present a continuation of our studies on the role of IHH-based signaling in early development and demonstrate that the IHH N-peptide is expressed in the outer visceral endoderm cells of both the EC and ES-derived embryoid body. We also show that Bmp2 is upregulated and Bmp4 downregulated during the differentiation of F9 EC cells into embryoid bodies, whereas both genes are upregulated when J7 ES cells differentiate into embryoid bodies. We also examine the spatial localization of Ihh, Bmp2, and Bmp4 in day 6.5-7.0 and 7.5-8.0 embryos by in situ hybridization analysis. These data support the EC temporal expression data in that all 3 genes are expressed in visceral endoderm. Bmp4 expression appears to be limited to extraembryonic regions, where mesoderm as well as visceral endoderm are stained. Ihh and Bmp2 are expressed in extraembryonic tissues and the embryo proper. Functional roles for the observed expression patterns are discussed.

  12. The AgroEcoSystem (AgES) response-function model simulates layered soil water dynamics in semi-arid Colorado: sensitivity and calibration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Simulation of vertical soil hydrology is a critical component of simulating even more complex soil water dynamics in space and time, including land-atmosphere and subsurface interactions. The AgroEcoSystem (AgES) model is defined here as a single land unit implementation of the full AgES-W (Watershe...

  13. RraAS2 requires both scaffold domains of RNase ES for high-affinity binding and inhibitory action on the ribonucleolytic activity.

    PubMed

    Heo, Jihune; Kim, Daeyoung; Joo, Minju; Lee, Boeun; Seo, Sojin; Lee, Jaejin; Song, Saemee; Yeom, Ji-Hyun; Ha, Nam-Chul; Lee, Kangseok

    2016-10-01

    RraA is a protein inhibitor of RNase E (Rne), which catalyzes the endoribonucleolytic cleavage of a large proportion of RNAs in Escherichia coli. The antibiotic-producing bacterium Streptomyces coelicolor also contains homologs of RNase E and RraA, designated as RNase ES (Rns), RraAS1, and RraAS2, respectively. Here, we report that RraAS2 requires both scaffold domains of RNase ES for high-affinity binding and inhibitory action on the ribonucleolytic activity. Analyses of the steady-state level of RNase E substrates indicated that coexpression of RraAS2 in E. coli cells overproducing Rns effectively inhibits the ribonucleolytic activity of full-length RNase ES, but its inhibitory effects were moderate or undetectable on other truncated forms of Rns, in which the N- or/and C-terminal scaffold domain was deleted. In addition, RraAS2 more efficiently inhibited the in vitro ribonucleolytic activity of RNase ES than that of a truncated form containing the catalytic domain only. Coimmunoprecipitation and in vivo cross-linking experiments further showed necessity of both scaffold domains of RNase ES for high-affinity binding of RraAS2 to the enzyme, resulting in decreased RNA-binding capacity of RNase ES. Our results indicate that RraAS2 is a protein inhibitor of RNase ES and provide clues to how this inhibitor affects the ribonucleolytic activity of RNase ES. PMID:27687228

  14. A component-based, integrated spatially distributed hydrologic/water quality model: AgroEcoSystem-Watershed (AgES-W) overview and application

    Technology Transfer Automated Retrieval System (TEKTRAN)

    AgroEcoSystem-Watershed (AgES-W) is a modular, Java-based spatially distributed model which implements hydrologic/water quality simulation components. The AgES-W model was previously evaluated for streamflow and recently has been enhanced with the addition of nitrogen (N) and sediment modeling compo...

  15. Le groupe de recherches transfusionnelles d’Afrique francophone: bilan des cinq premières années

    PubMed Central

    Tagny, Claude Tayou; Murphy, Edward L.; Lefrère, Jean-Jacques

    2016-01-01

    Les travaux de recherches sur la sécurité transfusionnelle en Afrique sub-saharienne sont peu nombreux, souvent limités à des initiatives locales avec des conclusions difficilement représentatives de cette région. Le Groupe de recherches transfusionnelles en Afrique sub-saharienne francophone a été créé en mai 2007 avec pour objectif de développer des stratégies globales d’amélioration de la sécurité transfusionnelle mais adaptables à la situation de chaque pays. Les activités du Groupe à ce jour ont porté essentiellement sur l’obtention de données épidémiologiques et de laboratoire sur la transfusion sanguine et à proposer des stratégies de sécurité transfusionnelle dans le domaine des infections transmissibles par la transfusion. Pour mener à bien ces activités de recherche, le Groupe travaille en étroite collaboration avec les Centres nationaux de transfusion sanguine (CNTS), les Centres régionaux de transfusion sanguine (CRTS), les banques de sang hospitalières (BSH) et les postes de collecte de sang. Pour les 5 premières années, quatre priorités de recherche ont été identifiées: (i) des études descriptives sur les caractéristiques des donneurs de sang et des centres de transfusion; (ii) une estimation du risque résiduel post-transfusionnel des principales infections virales transmissibles par la transfusion; (iii) une analyse des stratégies de sélection médicale des donneurs de sang; et (iv) une description des stratégies de dépistage des ITT et une description du système d’assurance qualité externe existant. Durant cette période, sept projets ont été mis en œuvre au niveau national et publiés et cinq études multicentriques ont été réalisées et publiées. La présente étude rapporte les principales observations et recommandations de ces études. PMID:24360798

  16. Mechanisms for GroEL/GroES-mediated folding of a large 86-kDa fusion polypeptide in vitro.

    PubMed

    Huang, Y S; Chuang, D T

    1999-04-01

    Our understanding of mechanisms for GroEL/GroES-assisted protein folding to date has been derived mostly from studies with small proteins. Little is known concerning the interaction of these chaperonins with large multidomain polypeptides during folding. In the present study, we investigated chaperonin-dependent folding of a large 86-kDa fusion polypeptide, in which the mature maltose-binding protein (MBP) sequence was linked to the N terminus of the alpha subunit of the decarboxylase (E1) component of the human mitochondrial branched-chain alpha-ketoacid dehydrogenase complex. The fusion polypeptide, MBP-alpha, when co-expressed with the beta subunit of E1, produced a chimeric protein MBP-E1 with an (MBP-alpha)2beta2 structure, similar to the alpha2 beta2 structure in native E1. Reactivation of MBP-E1 denatured in 8 M urea was absolutely dependent on GroEL/GroES and Mg2+-ATP, and exhibited strikingly slow kinetics with a rate constant of 376 M-1 s-1, analogous to denatured untagged E1. Chaperonin-mediated refolding of the MBP-alpha fusion polypeptide showed that the folding of the MBP moiety was about 7-fold faster than that of the alpha moiety on the same chain with rate constants of 1.9 x 10(-3) s-1 and 2.95 x 10(-4) s-1, respectively. This explained the occurrence of an MBP-alpha. GroEL binary complex that was isolated with amylose resin from the refolding mixture and transformed Escherichia coli lysates. The data support the thesis that distinct functional sequences in a large polypeptide exhibit different folding characteristics on the same GroEL scaffold. Moreover, we show that when the alpha.GroEL complex (molar ratio 1:1) was incubated with GroES, the latter was capable of capping either the very ring that harbored the 48-kDa (His)6-alpha polypeptide (in cis) or the opposite unoccupied cavity (in trans). In contrast, the MBP-alpha.GroEL (1:1) complex was capped by GroES exclusively in the trans configuration. These findings suggest that the productive

  17. Transcranial electric stimulation (tES) and NeuroImaging: the state-of-the-art, new insights and prospects in basic and clinical neuroscience.

    PubMed

    Soekadar, Surjo R; Herring, Jim Don; McGonigle, David

    2016-10-15

    Transcranial electric stimulation (tES) of the brain has attracted an increased interest in recent years. Yet, despite remarkable research efforts to date, the underlying neurobiological mechanisms of tES' effects are still incompletely understood. This Special Issue aims to provide a comprehensive and up-to-date overview of the state-of-the-art in studies combining tES and neuroimaging, while introducing most recent insights and outlining future prospects related to this new and rapidly growing field. The findings reported here combine methodological advancements with insights into the underlying mechanisms of tES itself. At the same time, they also point to the many caveats and specific challenges associated with such studies, which can arise from both technical and biological sources. Besides promising to advance basic neuroscience, combined tES and neuroimaging studies may also substantially change previous conceptions about the methods of action of electric or magnetic stimulation on the brain.

  18. Transcranial electric stimulation (tES) and NeuroImaging: the state-of-the-art, new insights and prospects in basic and clinical neuroscience.

    PubMed

    Soekadar, Surjo R; Herring, Jim Don; McGonigle, David

    2016-10-15

    Transcranial electric stimulation (tES) of the brain has attracted an increased interest in recent years. Yet, despite remarkable research efforts to date, the underlying neurobiological mechanisms of tES' effects are still incompletely understood. This Special Issue aims to provide a comprehensive and up-to-date overview of the state-of-the-art in studies combining tES and neuroimaging, while introducing most recent insights and outlining future prospects related to this new and rapidly growing field. The findings reported here combine methodological advancements with insights into the underlying mechanisms of tES itself. At the same time, they also point to the many caveats and specific challenges associated with such studies, which can arise from both technical and biological sources. Besides promising to advance basic neuroscience, combined tES and neuroimaging studies may also substantially change previous conceptions about the methods of action of electric or magnetic stimulation on the brain. PMID:27633745

  19. Profiling of microRNA in human and mouse ES and iPS cells reveals overlapping but distinct microRNA expression patterns.

    PubMed

    Razak, Siti Razila Abdul; Ueno, Kazuko; Takayama, Naoya; Nariai, Naoki; Nagasaki, Masao; Saito, Rika; Koso, Hideto; Lai, Chen-Yi; Murakami, Miyako; Tsuji, Koichiro; Michiue, Tatsuo; Nakauchi, Hiromitsu; Otsu, Makoto; Watanabe, Sumiko

    2013-01-01

    Using quantitative PCR-based miRNA arrays, we comprehensively analyzed the expression profiles of miRNAs in human and mouse embryonic stem (ES), induced pluripotent stem (iPS), and somatic cells. Immature pluripotent cells were purified using SSEA-1 or SSEA-4 and were used for miRNA profiling. Hierarchical clustering and consensus clustering by nonnegative matrix factorization showed two major clusters, human ES/iPS cells and other cell groups, as previously reported. Principal components analysis (PCA) to identify miRNAs that segregate in these two groups identified miR-187, 299-3p, 499-5p, 628-5p, and 888 as new miRNAs that specifically characterize human ES/iPS cells. Detailed direct comparisons of miRNA expression levels in human ES and iPS cells showed that several miRNAs included in the chromosome 19 miRNA cluster were more strongly expressed in iPS cells than in ES cells. Similar analysis was conducted with mouse ES/iPS cells and somatic cells, and several miRNAs that had not been reported to be expressed in mouse ES/iPS cells were suggested to be ES/iPS cell-specific miRNAs by PCA. Comparison of the average expression levels of miRNAs in ES/iPS cells in humans and mice showed quite similar expression patterns of human/mouse miRNAs. However, several mouse- or human-specific miRNAs are ranked as high expressers. Time course tracing of miRNA levels during embryoid body formation revealed drastic and different patterns of changes in their levels. In summary, our miRNA expression profiling encompassing human and mouse ES and iPS cells gave various perspectives in understanding the miRNA core regulatory networks regulating pluripotent cells characteristics.

  20. Effect of mitotic inducers and retinoic acid blocker on expression of pluripotent genes in ES cells derived from early stage in vitro-produced embryos in buffalo.

    PubMed

    Kumar, Ashok; Kumar, Kuldeep; Singh, Renu; Puri, Gopal; Ranjan, R; Yasotha, T; Singh, R K; Sarkar, M; Bag, Sadhan

    2012-12-01

    So far, it has been difficult to generate embryonic stem (ES) cell from early stage preimplantation embryos of buffalo. These ES cells will be more helpful for efficient embryo cloning and generation of body cells as they are more primitive than inner cell mass (ICM)-derived ES cells. The present study was conducted to find the effect of lipopolysaccharide (LPS), melatonin (N-acetyl-5-methoxytryptamine, a pineal gland product), and citral (3,7-dimethyl-2,6-octadienal and a retinoic acid synthesis blocker) on establishment of primary ES cell colonies, the comparative size of the ES cell colonies, and expression of pluripotent genes during extended period of culture in buffalo. Zona-free eight-cell stage in vitro fertilization (IVF) embryos were cultured in ES cell medium supplemented with none (media I as control), LPS (media II), citral melatonin (media III), or melatonin (media IV). The multiplication of blastomere leading to ES cell colony formation and expression of pluripotent genes were assessed up to day 20 of culture. The primary colony formation, the comparative size of the ES cell colonies, and expression of pluripotent genes in these colonies were better in the medium supplemented with melatonin in all days of culture. Within melatonin supplementation, the colony size was comparatively larger on day 8 and day 12 of culture. Further, with this supplementation, the Oct-4 and Nanog expression was comparatively higher on all days of culture. The results indicated that supplementation of melatonin helped in the formation of better primary ES cell colony as well as in the maintenance of pluripotency. The results also indicated that primary colonies developed on day 8 to day 12 of culture may be better for passaging them for establishment of ES cell line from early stage preimplantation IVF embryos of in buffalo.

  1. Effect of mitotic inducers and retinoic acid blocker on expression of pluripotent genes in ES cells derived from early stage in vitro-produced embryos in buffalo.

    PubMed

    Kumar, Ashok; Kumar, Kuldeep; Singh, Renu; Puri, Gopal; Ranjan, R; Yasotha, T; Singh, R K; Sarkar, M; Bag, Sadhan

    2012-12-01

    So far, it has been difficult to generate embryonic stem (ES) cell from early stage preimplantation embryos of buffalo. These ES cells will be more helpful for efficient embryo cloning and generation of body cells as they are more primitive than inner cell mass (ICM)-derived ES cells. The present study was conducted to find the effect of lipopolysaccharide (LPS), melatonin (N-acetyl-5-methoxytryptamine, a pineal gland product), and citral (3,7-dimethyl-2,6-octadienal and a retinoic acid synthesis blocker) on establishment of primary ES cell colonies, the comparative size of the ES cell colonies, and expression of pluripotent genes during extended period of culture in buffalo. Zona-free eight-cell stage in vitro fertilization (IVF) embryos were cultured in ES cell medium supplemented with none (media I as control), LPS (media II), citral melatonin (media III), or melatonin (media IV). The multiplication of blastomere leading to ES cell colony formation and expression of pluripotent genes were assessed up to day 20 of culture. The primary colony formation, the comparative size of the ES cell colonies, and expression of pluripotent genes in these colonies were better in the medium supplemented with melatonin in all days of culture. Within melatonin supplementation, the colony size was comparatively larger on day 8 and day 12 of culture. Further, with this supplementation, the Oct-4 and Nanog expression was comparatively higher on all days of culture. The results indicated that supplementation of melatonin helped in the formation of better primary ES cell colony as well as in the maintenance of pluripotency. The results also indicated that primary colonies developed on day 8 to day 12 of culture may be better for passaging them for establishment of ES cell line from early stage preimplantation IVF embryos of in buffalo. PMID:23093464

  2. ETS-related transcription factors ETV4 and ETV5 are involved in proliferation and induction of differentiation-associated genes in embryonic stem (ES) cells.

    PubMed

    Akagi, Tadayuki; Kuure, Satu; Uranishi, Kousuke; Koide, Hiroshi; Costantini, Frank; Yokota, Takashi

    2015-09-11

    The pluripotency and self-renewal capacity of embryonic stem (ES) cells is regulated by several transcription factors. Here, we show that the ETS-related transcription factors Etv4 and Etv5 (Etv4/5) are specifically expressed in undifferentiated ES cells, and suppression of Oct3/4 results in down-regulation of Etv4/5. Simultaneous deletion of Etv4 and Etv5 (Etv4/5 double knock-out (dKO)) in ES cells resulted in a flat, epithelial cell-like appearance, whereas the morphology changed into compact colonies in a 2i medium (containing two inhibitors for GSK3 and MEK/ERK). Expression levels of self-renewal marker genes, including Oct3/4 and Nanog, were similar between wild-type and dKO ES cells, whereas proliferation of Etv4/5 dKO ES cells was decreased with overexpression of cyclin-dependent kinase inhibitors (p16/p19, p15, and p57). A differentiation assay revealed that the embryoid bodies derived from Etv4/5 dKO ES cells were smaller than the control, and expression of ectoderm marker genes, including Fgf5, Sox1, and Pax3, was not induced in dKO-derived embryoid bodies. Microarray analysis demonstrated that stem cell-related genes, including Tcf15, Gbx2, Lrh1, Zic3, and Baf60c, were significantly repressed in Etv4/5 dKO ES cells. The artificial expression of Etv4 and/or Etv5 in Etv4/5 dKO ES cells induced re-expression of Tcf15 and Gbx2. These results indicate that Etv4 and Etv5, potentially through regulation of Gbx2 and Tcf15, are involved in the ES cell proliferation and induction of differentiation-associated genes in ES cells.

  3. ETS-related Transcription Factors ETV4 and ETV5 Are Involved in Proliferation and Induction of Differentiation-associated Genes in Embryonic Stem (ES) Cells*

    PubMed Central

    Akagi, Tadayuki; Kuure, Satu; Uranishi, Kousuke; Koide, Hiroshi; Costantini, Frank; Yokota, Takashi

    2015-01-01

    The pluripotency and self-renewal capacity of embryonic stem (ES) cells is regulated by several transcription factors. Here, we show that the ETS-related transcription factors Etv4 and Etv5 (Etv4/5) are specifically expressed in undifferentiated ES cells, and suppression of Oct3/4 results in down-regulation of Etv4/5. Simultaneous deletion of Etv4 and Etv5 (Etv4/5 double knock-out (dKO)) in ES cells resulted in a flat, epithelial cell-like appearance, whereas the morphology changed into compact colonies in a 2i medium (containing two inhibitors for GSK3 and MEK/ERK). Expression levels of self-renewal marker genes, including Oct3/4 and Nanog, were similar between wild-type and dKO ES cells, whereas proliferation of Etv4/5 dKO ES cells was decreased with overexpression of cyclin-dependent kinase inhibitors (p16/p19, p15, and p57). A differentiation assay revealed that the embryoid bodies derived from Etv4/5 dKO ES cells were smaller than the control, and expression of ectoderm marker genes, including Fgf5, Sox1, and Pax3, was not induced in dKO-derived embryoid bodies. Microarray analysis demonstrated that stem cell-related genes, including Tcf15, Gbx2, Lrh1, Zic3, and Baf60c, were significantly repressed in Etv4/5 dKO ES cells. The artificial expression of Etv4 and/or Etv5 in Etv4/5 dKO ES cells induced re-expression of Tcf15 and Gbx2. These results indicate that Etv4 and Etv5, potentially through regulation of Gbx2 and Tcf15, are involved in the ES cell proliferation and induction of differentiation-associated genes in ES cells. PMID:26224636

  4. HD-03/ES: A Herbal Medicine Inhibits Hepatitis B Surface Antigen Secretion in Transfected Human Hepatocarcinoma PLC/PRF/5 Cells.

    PubMed

    Varma, Sandeep R; Sundaram, R; Gopumadhavan, S; Vidyashankar, Satyakumar; Patki, Pralhad S

    2013-01-01

    HD-03/ES is a herbal formulation used for the treatment of hepatitis B. However, the molecular mechanism involved in the antihepatitis B (HBV) activity of this drug has not been studied using in vitro models. The effect of HD-03/ES on hepatitis B surface antigen (HBsAg) secretion and its gene expression was studied in transfected human hepatocarcinoma PLC/PRF/5 cells. The anti-HBV activity was tested based on the inhibition of HBsAg secretion into the culture media, as detected by HBsAg-specific antibody-mediated enzyme assay (ELISA) at concentrations ranging from 125 to 1000  μ g/mL. The effect of HD-03/ES on HBsAg gene expression was analyzed using semiquantitative multiplex RT-PCR by employing specific primers. The results showed that HD-03/ES suppressed HBsAg production with an IC50 of 380  μ g/mL in PLC/PRF/5 cells for a period of 24 h. HD-03/ES downregulated HBsAg gene expression in PLC/PRF/5 cells. In conclusion, HD-03/ES exhibits strong anti-HBV properties by inhibiting the secretion of hepatitis B surface antigen in PLC/PRF/5 cells, and this action is targeted at the transcription level. Thus, HD-03/ES could be beneficial in the treatment of acute and chronic hepatitis B infections.

  5. Germ Cell Nuclear Factor (GCNF) Represses Oct4 Expression and Globally Modulates Gene Expression in Human Embryonic Stem (hES) Cells*

    PubMed Central

    Wang, Hongran; Wang, Xiaohong; Xu, Xueping; Kyba, Michael; Cooney, Austin J.

    2016-01-01

    Oct4 is considered a key transcription factor for pluripotent stem cell self-renewal. It binds to specific regions within target genes to regulate their expression and is downregulated upon induction of differentiation of pluripotent stem cells; however, the mechanisms that regulate the levels of human Oct4 expression remain poorly understood. Here we show that expression of human Oct4 is directly repressed by germ cell nuclear factor (GCNF), an orphan nuclear receptor, in hES cells. Knockdown of GCNF by siRNA resulted in maintenance of Oct4 expression during RA-induced hES cell differentiation. While overexpression of GCNF promoted repression of Oct4 expression in both undifferentiated and differentiated hES cells. The level of Oct4 repression was dependent on the level of GCNF expression in a dose-dependent manner. mRNA microarray analysis demonstrated that overexpression of GCNF globally regulates gene expression in undifferentiated and differentiated hES cells. Within the group of altered genes, GCNF down-regulated 36% of the genes, and up-regulated 64% in undifferentiated hES cells. In addition, GCNF also showed a regulatory gene pattern that is different from RA treatment during hES cell differentiation. These findings increase our understanding of the mechanisms that maintain hES cell pluripotency and regulate gene expression during the differentiation process. PMID:26769970

  6. Germ Cell Nuclear Factor (GCNF) Represses Oct4 Expression and Globally Modulates Gene Expression in Human Embryonic Stem (hES) Cells.

    PubMed

    Wang, Hongran; Wang, Xiaohong; Xu, Xueping; Kyba, Michael; Cooney, Austin J

    2016-04-15

    Oct4 is considered a key transcription factor for pluripotent stem cell self-renewal. It binds to specific regions within target genes to regulate their expression and is downregulated upon induction of differentiation of pluripotent stem cells; however, the mechanisms that regulate the levels of human Oct4 expression remain poorly understood. Here we show that expression of human Oct4 is directly repressed by germ cell nuclear factor (GCNF), an orphan nuclear receptor, in hES cells. Knockdown of GCNF by siRNA resulted in maintenance of Oct4 expression during RA-induced hES cell differentiation. While overexpression of GCNF promoted repression of Oct4 expression in both undifferentiated and differentiated hES cells. The level of Oct4 repression was dependent on the level of GCNF expression in a dose-dependent manner. mRNA microarray analysis demonstrated that overexpression of GCNF globally regulates gene expression in undifferentiated and differentiated hES cells. Within the group of altered genes, GCNF down-regulated 36% of the genes, and up-regulated 64% in undifferentiated hES cells. In addition, GCNF also showed a regulatory gene pattern that is different from RA treatment during hES cell differentiation. These findings increase our understanding of the mechanisms that maintain hES cell pluripotency and regulate gene expression during the differentiation process.

  7. HD-03/ES: A Herbal Medicine Inhibits Hepatitis B Surface Antigen Secretion in Transfected Human Hepatocarcinoma PLC/PRF/5 Cells

    PubMed Central

    Varma, Sandeep R.; Sundaram, R.; Gopumadhavan, S.; Vidyashankar, Satyakumar; Patki, Pralhad S.

    2013-01-01

    HD-03/ES is a herbal formulation used for the treatment of hepatitis B. However, the molecular mechanism involved in the antihepatitis B (HBV) activity of this drug has not been studied using in vitro models. The effect of HD-03/ES on hepatitis B surface antigen (HBsAg) secretion and its gene expression was studied in transfected human hepatocarcinoma PLC/PRF/5 cells. The anti-HBV activity was tested based on the inhibition of HBsAg secretion into the culture media, as detected by HBsAg-specific antibody-mediated enzyme assay (ELISA) at concentrations ranging from 125 to 1000 μg/mL. The effect of HD-03/ES on HBsAg gene expression was analyzed using semiquantitative multiplex RT-PCR by employing specific primers. The results showed that HD-03/ES suppressed HBsAg production with an IC50 of 380 μg/mL in PLC/PRF/5 cells for a period of 24 h. HD-03/ES downregulated HBsAg gene expression in PLC/PRF/5 cells. In conclusion, HD-03/ES exhibits strong anti-HBV properties by inhibiting the secretion of hepatitis B surface antigen in PLC/PRF/5 cells, and this action is targeted at the transcription level. Thus, HD-03/ES could be beneficial in the treatment of acute and chronic hepatitis B infections. PMID:23691296

  8. Window screening, ceilings and closed eaves as sustainable ways to control malaria in Dar es Salaam, Tanzania

    PubMed Central

    Ogoma, Sheila B; Kannady, Khadija; Sikulu, Maggy; Chaki, Prosper P; Govella, Nicodem J; Mukabana, Wolfgang R; Killeen, Gerry F

    2009-01-01

    Background Malaria transmission in Africa occurs predominantly inside houses where the primary vectors prefer to feed. Human preference and investment in blocking of specific entry points for mosquitoes into houses was evaluated and compared with known entry point preferences of the mosquitoes themselves. Methods Cross-sectional household surveys were conducted in urban Dar es Salaam, Tanzania to estimate usage levels of available options for house proofing against mosquito entry, namely window screens, ceilings and blocking of eaves. These surveys also enabled evaluation of household expenditure on screens and ceilings and the motivation behind their installation. Results Over three quarters (82.8%) of the 579 houses surveyed in Dar es Salaam had window screens, while almost half (48.9%) had ceilings. Prevention of mosquito entry was cited as a reason for installation of window screens and ceilings by 91.4% (394/431) and 55.7% (127/228) of respondents, respectively, but prevention of malaria was rarely cited (4.3%, 22/508). The median cost of window screens was between US $ 21-30 while that of ceilings was between US $301-400. The market value of insecticide-treated nets, window screening and ceilings currently in use in the city was estimated as 2, 5 and 42 million US$. More than three quarters of the respondents that lacked them said it was too expensive to install ceilings (82.2%) or window screens (75.5%). Conclusion High coverage and spending on screens and ceilings implies that these techniques are highly acceptable and excellent uptake can be achieved in urban settings like Dar es Salaam. Effective models for promotion and subsidization should be developed and evaluated, particularly for installation of ceilings that prevent entry via the eaves, which are the most important entry point for mosquitoes that cause malaria, a variety of neglected tropical diseases and the nuisance which motivates uptake. PMID:19785779

  9. FGF7 and cell density are required for final differentiation of pancreatic amylase-positive cells from human ES cells.

    PubMed

    Takizawa-Shirasawa, Sakiko; Yoshie, Susumu; Yue, Fengming; Mogi, Akimi; Yokoyama, Tadayuki; Tomotsune, Daihachiro; Sasaki, Katsunori

    2013-12-01

    The major molecular signals of pancreatic exocrine development are largely unknown. We examine the role of fibroblast growth factor 7 (FGF7) in the final induction of pancreatic amylase-containing exocrine cells from induced-pancreatic progenitor cells derived from human embryonic stem (hES) cells. Our protocol consisted in three steps: Step I, differentiation of definitive endoderm (DE) by activin A treatment of hES cell colonies; Step II, differentiation of pancreatic progenitor cells by re-plating of the cells of Step I onto 24-well plates at high density and stimulation with all-trans retinoic acid; Step III, differentiation of pancreatic exocrine cells with a combination of FGF7, glucagon-like peptide 1 and nicotinamide. The expression levels of pancreatic endodermal markers such as Foxa2, Sox17 and gut tube endoderm marker HNF1β were up-regulated in both Step I and II. Moreover, in Step III, the induced cells expressed pancreatic markers such as amylase, carboxypeptidase A and chymotrypsinogen B, which were similar to those in normal human pancreas. From day 8 in Step III, cells immunohistochemically positive for amylase and for carboxypeptidase A, a pancreatic exocrine cell product, were induced by FGF7. Pancreatic progenitor Pdx1-positive cells were localized in proximity to the amylase-positive cells. In the absence of FGF7, few amylase-positive cells were identified. Thus, our three-step culture protocol for human ES cells effectively induces the differentiation of amylase- and carboxypeptidase-A-containing pancreatic exocrine cells.

  10. Hexavalent chromium reduction by Cellulomonas sp. strain ES6: the influence of carbon source, iron minerals, and electron shuttling compounds.

    PubMed

    Field, Erin K; Gerlach, Robin; Viamajala, Sridhar; Jennings, Laura K; Peyton, Brent M; Apel, William A

    2013-06-01

    The reduction of hexavalent chromium, Cr(VI), to trivalent chromium, Cr(III), can be an important aspect of remediation processes at contaminated sites. Cellulomonas species are found at several Cr(VI) contaminated and uncontaminated locations at the Department of Energy site in Hanford, Washington. Members of this genus have demonstrated the ability to effectively reduce Cr(VI) to Cr(III) fermentatively and therefore play a potential role in Cr(VI) remediation at this site. Batch studies were conducted with Cellulomonas sp. strain ES6 to assess the influence of various carbon sources, iron minerals, and electron shuttling compounds on Cr(VI) reduction rates as these chemical species are likely to be present in, or added to, the environment during in situ bioremediation. Results indicated that the type of carbon source as well as the type of electron shuttle present influenced Cr(VI) reduction rates. Molasses stimulated Cr(VI) reduction more effectively than pure sucrose, presumably due to presence of more easily utilizable sugars, electron shuttling compounds or compounds with direct Cr(VI) reduction capabilities. Cr(VI) reduction rates increased with increasing concentration of anthraquinone-2,6-disulfonate (AQDS) regardless of the carbon source. The presence of iron minerals and their concentrations did not significantly influence Cr(VI) reduction rates. However, strain ES6 or AQDS could directly reduce surface-associated Fe(III) to Fe(II), which was capable of reducing Cr(VI) at a near instantaneous rate. These results suggest the rate limiting step in these systems was the transfer of electrons from strain ES6 to the intermediate or terminal electron acceptor whether that was Cr(VI), Fe(III), or AQDS.

  11. Completely ES cell-derived mice produced by tetraploid complementation using inner cell mass (ICM) deficient blastocysts.

    PubMed

    Wen, Duancheng; Saiz, Nestor; Rosenwaks, Zev; Hadjantonakis, Anna-Katerina; Rafii, Shahin

    2014-01-01

    Tetraploid complementation is often used to produce mice from embryonic stem cells (ESCs) by injection of diploid (2n) ESCs into tetraploid (4n) blastocysts (ESC-derived mice). This method has also been adapted to mouse cloning and the derivation of mice from induced pluripotent stem (iPS) cells. However, the underlying mechanism(s) of the tetraploid complementation remains largely unclear. Whether this approach can give rise to completely ES cell-derived mice is an open question, and has not yet been unambiguously proven. Here, we show that mouse tetraploid blastocysts can be classified into two groups, according to the presence or absence of an inner cell mass (ICM). We designate these as type a (presence of ICM at blastocyst stage) or type b (absence of ICM). ESC lines were readily derived from type a blastocysts, suggesting that these embryos retain a pluripotent epiblast compartment; whereas the type b blastocysts possessed very low potential to give rise to ESC lines, suggesting that they had lost the pluripotent epiblast. When the type a blastocysts were used for tetraploid complementation, some of the resulting mice were found to be 2n/4n chimeric; whereas when type b blastocysts were used as hosts, the resulting mice are all completely ES cell-derived, with the newborn pups displaying a high frequency of abdominal hernias. Our results demonstrate that completely ES cell-derived mice can be produced using ICM-deficient 4n blastocysts, and provide evidence that the exclusion of tetraploid cells from the fetus in 2n/4n chimeras can largely be attributed to the formation of ICM-deficient blastocysts.

  12. Impact of Community-Based Larviciding on the Prevalence of Malaria Infection in Dar es Salaam, Tanzania

    PubMed Central

    Maheu-Giroux, Mathieu; Castro, Marcia C.

    2013-01-01

    Background The use of larval source management is not prioritized by contemporary malaria control programs in sub-Saharan Africa despite historical success. Larviciding, in particular, could be effective in urban areas where transmission is focal and accessibility to Anopheles breeding habitats is generally easier than in rural settings. The objective of this study is to assess the effectiveness of a community-based microbial larviciding intervention to reduce the prevalence of malaria infection in Dar es Salaam, United Republic of Tanzania. Methods and Findings Larviciding was implemented in 3 out of 15 targeted wards of Dar es Salaam in 2006 after two years of baseline data collection. This intervention was subsequently scaled up to 9 wards a year later, and to all 15 targeted wards in 2008. Continuous randomized cluster sampling of malaria prevalence and socio-demographic characteristics was carried out during 6 survey rounds (2004–2008), which included both cross-sectional and longitudinal data (N = 64,537). Bayesian random effects logistic regression models were used to quantify the effect of the intervention on malaria prevalence at the individual level. Effect size estimates suggest a significant protective effect of the larviciding intervention. After adjustment for confounders, the odds of individuals living in areas treated with larviciding being infected with malaria were 21% lower (Odds Ratio = 0.79; 95% Credible Intervals: 0.66–0.93) than those who lived in areas not treated. The larviciding intervention was most effective during dry seasons and had synergistic effects with other protective measures such as use of insecticide-treated bed nets and house proofing (i.e., complete ceiling or window screens). Conclusion A large-scale community-based larviciding intervention significantly reduced the prevalence of malaria infection in urban Dar es Salaam. PMID:23977099

  13. Interdependence of domestic malaria prevention measures and mosquito-human interactions in urban Dar es Salaam, Tanzania

    PubMed Central

    Geissbühler, Yvonne; Chaki, Prosper; Emidi, Basiliana; Govella, Nicodemus J; Shirima, Rudolf; Mayagaya, Valeliana; Mtasiwa, Deo; Mshinda, Hassan; Fillinger, Ulrike; Lindsay, Steven W; Kannady, Khadija; de Castro, Marcia Caldas; Tanner, Marcel; Killeen, Gerry F

    2007-01-01

    Background Successful malaria vector control depends on understanding behavioural interactions between mosquitoes and humans, which are highly setting-specific and may have characteristic features in urban environments. Here mosquito biting patterns in Dar es Salaam, Tanzania are examined and the protection against exposure to malaria transmission that is afforded to residents by using an insecticide-treated net (ITN) is estimated. Methods Mosquito biting activity over the course of the night was estimated by human landing catch in 216 houses and 1,064 residents were interviewed to determine usage of protection measures and the proportion of each hour of the night spent sleeping indoors, awake indoors, and outdoors. Results Hourly variations in biting activity by members of the Anopheles gambiae complex were consistent with classical reports but the proportion of these vectors caught outdoors in Dar es Salaam was almost double that of rural Tanzania. Overall, ITNs confer less protection against exophagic vectors in Dar es Salaam than in rural southern Tanzania (59% versus 70%). More alarmingly, a biting activity maximum that precedes 10 pm and much lower levels of ITN protection against exposure (38%) were observed for Anopheles arabiensis, a vector of modest importance locally, but which predominates transmission in large parts of Africa. Conclusion In a situation of changing mosquito and human behaviour, ITNs may confer lower, but still useful, levels of personal protection which can be complemented by communal transmission suppression at high coverage. Mosquito-proofing houses appeared to be the intervention of choice amongst residents and further options for preventing outdoor transmission include larviciding and environmental management. PMID:17880679

  14. Influence of Carbon Sources and Electron Shuttles on Ferric Iron Reduction by Cellulomonas sp. Strain ES6

    SciTech Connect

    Erin K. Field; Robin Gerlach; Sridhar Viamajala; Laura K. Jennings; Alfred B. Cunningham; Brent M. Peyton; William A. Apel

    2011-09-01

    The reduction of hexavalent chromium, Cr(VI), to trivalent chromium, Cr(III), can be an important aspect of remediation processes at Department of Energy (DOE) and other contaminated sites. Cellulomonas species are found at several Cr(VI) contaminated and uncontaminated locations at the DOE site in Hanford, Washington. Members of this genus have demonstrated the ability to effectively reduce Cr(VI) to Cr(III) fermentatively and therefore play a potential role in hexavalent chromium remediation at this site. Batch studies were conducted with Cellulomonas sp. strain ES6 to assess the influence of various carbon sources, iron minerals, and electron shuttling compounds on Cr(VI) reduction. These chemical species are likely to be present in these terrestrial environments during in situ bioremediation. Results indicated that there were a number of interactions between these compounds that influenced Cr(VI) reduction rates. The type of carbon source as well as the type of electron shuttle present influenced Cr(VI) reduction rates. When an electron shuttle, such as anthraquinone-2,6-disulfonate (AQDS), was present in the system, reduction rates increased significantly. Biologically reduced AQDS (AHDS) reduced Cr(VI) almost instantaneously. The presence of iron minerals and their concentrations did not significantly influence Cr(VI) reduction rates. However, strain ES6 or AQDS could directly reduce surface-associated Fe(III) to Fe(II) which was capable of reducing Cr(VI) at a near instantaneous rate. These results suggest the rate limiting step in these systems is the transfer of electrons from strain ES6 to the intermediate or terminal electron acceptor whether that is Cr(VI), Fe(III), or AQDS.

  15. PHOTOMETRICALLY DERIVED MASSES AND RADII OF THE PLANET AND STAR IN THE TrES-2 SYSTEM

    SciTech Connect

    Barclay, Thomas; Huber, Daniel; Rowe, Jason F.; Quintana, Elisa V.; Christiansen, Jessie L.; Jenkins, Jon M.; Mullally, Fergal; Seader, Shaun E.; Tenenbaum, Peter; Thompson, Susan E.; Barentsen, Geert; Bloemen, Steven; Demory, Brice-Olivier; Fulton, Benjamin J.; Shporer, Avi; Ragozzine, Darin

    2012-12-10

    We measure the mass and radius of the star and planet in the TrES-2 system using 2.7 years of observations by the Kepler spacecraft. The light curve shows evidence for ellipsoidal variations and Doppler beaming on a period consistent with the orbital period of the planet with amplitudes of 2.79{sup +0.44}{sub -0.62} and 3.44{sup +0.32}{sub -0.37} parts per million (ppm), respectively, and a difference between the dayside and the nightside planetary flux of 3.41{sup +0.55}{sub -0.82} ppm. We present an asteroseismic analysis of solar-like oscillations on TrES-2A which we use to calculate the stellar mass of 0.94 {+-} 0.05 M{sub Sun} and radius of 0.95 {+-} 0.02 R{sub Sun }. Using these stellar parameters, a transit model fit and the phase-curve variations, we determine the planetary radius of 1.162{sup +0.020}{sub -0.024} R{sub Jup} and derive a mass for TrES-2b from the photometry of 1.44 {+-} 0.21 M{sub Jup}. The ratio of the ellipsoidal variation to the Doppler beaming amplitudes agrees to better than 2{sigma} with theoretical predications, while our measured planet mass and radius agree within 2{sigma} of previously published values based on spectroscopic radial velocity measurements. We measure a geometric albedo of 0.0136{sup +0.0022}{sub -0.0033} and an occultation (secondary eclipse) depth of 6.5{sup +1.7}{sub -1.8} ppm which we combined with the day/night planetary flux ratio to model the atmosphere of TrES-2b. We find that an atmosphere model that contains a temperature inversion is strongly preferred. We hypothesize that the Kepler bandpass probes a significantly greater atmospheric depth on the night side relative to the day side.

  16. Search for superheavy elements using the /sup 48/Ca+/sup 254/Es/sup g/ reaction

    SciTech Connect

    Lougheed, R.W.; Landrum, J.H.; Hulet, E.K.; Wild, J.F.; Dougan, R.J.; Dougan, A.D.; Gaeggeler, H.; Schaedel, M.; Moody, K.J.; Gregorich, K.E.

    1985-11-01

    We measured upper limits for the production of superheavy elements from the compound nucleus reaction /sup 48/Ca+/sup 254/Es. This combination permits the closest approach to the predicted island of stability at the 184 closed-neutron shell of any practical fusion reaction. We used aqueous and gas-phase separations to isolate superheavy element fractions within an hour from the end of the bombardments. In these experiments we did not observe superheavy elements above a limit of 3 x 10/sup -31/ cm/sup 2/ for spontaneous-fission half-lives from fractions of a day to a few months.

  17. Pathologies cutanées vues au laboratoire d'anatomie pathologique à Lomé, Togo

    PubMed Central

    Darre, Tchin; Mouhari-Toure, Abas; Saka, Bayaki; Amouzou, Efoé-ga Yawod Olivier; Dare, Sassil; Landoh, Dadja Essoya; Amegbor, Koffi; Pitché, Palokinam; Napo-Koura, Gado

    2015-01-01

    Introduction Les affections cutanées restent encore un problème de santé publique dans la majorité des pays en développement. Notre étude s'est fixée comme objectif de déterminer les aspects épidémiologiques et histologiques des dermatoses au Togo. Méthodes Il s'agissait d'une étude descriptive et transversale portant sur les cas de dermatoses diagnostiquées de 2002 à 2013 (10 ans) au laboratoire d'anatomie pathologique (LAP) du CHU- Sylvanus Olympio. Tous les cas d'examen portant sur un prélèvement de peau (biopsie, exérèse, pièces opératoires) ont été colligés de 2002 à 2013 à partir des données des registres dudit laboratoire. Résultats Au cours de la période d’étude, 1119 (7,6%) des 14720 prélèvements reçus au LAP étaient des prélèvements de peau, ce qui correspond à une fréquence annuelle de 111,9 prélèvements. L’âge moyen des patients dont les prélèvements de peau appartenaient était de 35,4 ans et le sex-ratio (H/F) de 1,39. Au plan histologique, les lésions cutanées étaient reparties en dermatoses non tumorales (390 cas, 34,8%), dermatoses tumorales et pseudo tumorales (607 cas, 54,2%) et des dermatoses de diagnostic incertain (122 cas, 10,9%). La lèpre (95 cas, 8,5%) et le carcinome épidermoïde (134 cas, 11,9%) étaient les types histologiques les plus fréquents. Conclusion Les dermatoses tumorales et pseudo tumorales font plus l'objet de demande d'examen anatomopathologique au Togo, ce qui s'explique par la hantise de tumeurs malignes devant toute dermatose tumorale. L'amélioration du plateau technique du LAP (immunohistochimie, immunofluorescence directe) permettra d'accroitre ses capacités diagnostiques. PMID:26405477

  18. EXTENDED ULTRAVIOLET DISKS AND ULTRAVIOLET-BRIGHT DISKS IN LOW-MASS E/S0 GALAXIES

    SciTech Connect

    Moffett, Amanda J.; Kannappan, Sheila J.; Baker, Andrew J.; Laine, Seppo

    2012-01-20

    We have identified 15 extended ultraviolet (XUV) disks in a largely field sample of 38 E/S0 galaxies that have stellar masses primarily below {approx}4 Multiplication-Sign 10{sup 10} M{sub Sun} and comparable numbers on the red and blue sequences. We use a new purely quantitative XUV-disk definition designed with reference to the 'Type 1' XUV-disk definition found in the literature, requiring UV extension relative to a UV-defined star formation threshold radius. The 39% {+-} 9% XUV-disk frequency for these E/S0s is roughly twice the {approx}20% reported for late-type galaxies (although differences in XUV-disk criteria complicate the comparison), possibly indicating that XUV disks are preferentially associated with galaxies experiencing weak or inefficient star formation. Consistent with this interpretation, we find that the XUV disks in our sample do not correlate with enhanced outer-disk star formation as traced by blue optical outer-disk colors. However, UV-Bright (UV-B) disk galaxies with blue UV colors outside their optical 50% light radii do display enhanced optical outer-disk star formation as well as enhanced atomic gas content. UV-B disks occur in our E/S0s with a 42{sup +9}{sub -8}% frequency and need not coincide with XUV disks; thus their combined frequency is 61% {+-} 9%. For both XUV and UV-B disks, UV colors typically imply <1 Gyr ages, and most such disks extend beyond the optical R{sub 25} radius. XUV disks occur over the full sample mass range and on both the red and blue sequences, suggesting an association with galaxy interactions or another similarly general evolutionary process. In contrast, UV-B disks favor the blue sequence and may also prefer low masses, perhaps reflecting the onset of cold-mode gas accretion or another mass-dependent evolutionary process. Virtually all blue E/S0s in the gas-rich regime below stellar mass M{sub t} {approx} 5 Multiplication-Sign 10{sup 9} M{sub Sun} (the 'gas-richness threshold mass') display UV-B disks

  19. Métastases cutanées vues au laboratoire d'anatomie pathologique à Lomé, Togo entre 2005 et 2014

    PubMed Central

    Darre, Tchin; Saka, Bayaki; Landoh, Dadja Essoya; Mouhari-Toure, Abas; Amegbor, Koffi; Pitché, Palokinam; Napo-Koura, Gado

    2015-01-01

    Introduction L'objectif de cette étude était de documenter le profil épidémiologique et histologique des métastases cutanées vues au laboratoire d'anatomie pathologique (LAP) de Lomé. Méthodes Il s'agissait d'une étude descriptive et transversale portant sur des cas de métastases cutanées observées au LAP du CHU Sylvanus Olympio entre 2005 et 2014. Résultats Au cours de cette période d’étude, nous avons recensé 32 cas de métastases cutanées représentant 3,2% (1005 cas) de l'ensemble des cas de métastases enregistrées au LAP. L’âge moyen des patients atteints de ces métastases cutanées était de 42,6 ans et le sex-ratio (F/H) de 2,2. Sur le plan macroscopique, ces métastases étaient nodulaires dans 15 cas, bourgeonnantes dans 12 cas, ulcéré dans 3 cas et ulcéro-bourgeonnant dans 2 cas. Les types histologiques étaient représentés par les adénocarcinomes (19 cas, 59,4%), les carcinomes épidermoïdes (8 cas, 25%), la maladie de Paget (3 cas, 9,4%), le carcinome à petites cellules du type neuroendocrine (un cas, 3,1%) et le mélanome (un cas, 3,1%). Selon le degré de différenciation, les métastases cutanées étaient bien différenciées dans 14 cas (56%). Les principales localisations de ces métastases cutanées étaient le thorax (11 cas, 34,4%) suivi de l'abdomen (10 cas, 31,3%). Les cancers primitifs étaient surtout d'origine mammaire (14 cas, 43,7%). Conclusion Cette étude montre que les métastases cutanées sont relativement rares et les tumeurs malignes d'origine mammaire sont les tumeurs primitives prédominantes. Avec un pourcentage élevé des formes peu différenciées et celles indifférenciées, l'amélioration du plateau technique du LAP (immunohistochimie) permettra d'accroitre ses capacités diagnostiques. PMID:27047627

  20. Fission cross section measurements of Cm-247, Cf-250 and Es-254 from 0. 1 eV to 80 keV

    SciTech Connect

    Danon, Y.; Slovacek, R.E.; Block, R.C. . Dept. of Nuclear Engineering and Engineering Physics); Lougheed, R.W.; Hoff, R.W. ); Moore, M.S. )

    1990-01-01

    Fission cross section measurements were made with the RINS system over the neutron energy range from approximately 0.1 eV to 80 keV upon samples of Cm-247, Cf-250 and Es-254. The Cm-247 measurement was undertaken to complete the RINS fission cross section measurement sequence of the curium isotopes, Es-254 was measured because it is a very heavy odd-odd nucleus which might show interesting nuclear structure effects in its fission cross section, and Cf-250 was measured to account for its buildup as a daughter product from the 276-day halflife Es-254. 6 refs., 3 figs.

  1. The Hadronic Origin of the Hard Gamma-Ray Spectrum from Blazar 1ES 1101-232

    NASA Astrophysics Data System (ADS)

    Cao, Gang; Wang, Jiancheng

    2014-03-01

    The very hard γ-ray spectrum from distant blazars challenges the traditional synchrotron self-Compton (SSC) model, which may indicate that there is a contribution from an additional high-energy component beyond the SSC emission. In this paper, we study the possible origin of the hard γ-ray spectrum from distant blazars. We develop a model to explain the hard γ-ray spectrum from blazar 1ES 1101-232. In the model, the optical and X-ray radiation would come from the synchrotron radiation of primary electrons and secondary pairs and the GeV emission would be produced by the SSC process, however, the hard γ-ray spectrum would originate from the decay of neutral pion produced through proton-photon interactions with the synchrotron radiation photons within the jet. Our model can explain the observed spectral energy distribution of 1ES 1101-232 well, especially the very hard γ-ray spectrum. However, our model requires a very large proton power to efficiently produce the γ-ray through proton-photon interactions.

  2. [Isolation and nuclear transfer of ES-like cells colonies derived from embryos being cloning of bovine somatic].

    PubMed

    Dong, Ya-Juan; Bai, Xue-Jin; Li, Jian-Dong; Suzuki, Tatsuyuki

    2003-02-01

    In this experiment, it was designed to carry out proliferous culture of bovine blastocysts(day 7) derived from embryos cloned through bovine somatic cell nuclear transfer, isolating and passaging of ES cells. The cells of blastocysts, which were planted on feeder layer, formed small colonies within 24 h. The nest-shape colonies occurred after culturing for 2-3 days. After the colonies in the same shape were isolated and passaged 4-5 times, many different size colonies with monolayer of multi-cells appeared. The colonies that had been passaged 4-5 times were planted into 4-wells multi-dishes without feeder layer. The colonies with monolayer of multi-cells appeared after 24 h, spread all over the bottom of the dishes, emerged epidermis-like cells that appeared reticulate after 4-7 days. These cells were used as donor cells to carry out nuclear transfer. The results showed that 80% (40/50) of the reconstructed embryos cleaved, 5% (2/40) and 2.5% (1/40) of them developed to the morulaes and blastocyst stage, respectively. It revealed that ES-like cells derived embryos constructed through somatic cell nuclear transfer have the developmental potentials.

  3. Weather Information Communication (WINCOMM) VDL-3 and 1090ES Final Test Requirements, Test Plans, and Test Results

    NASA Technical Reports Server (NTRS)

    Griner, James H.; Jirberg, Russ; Frantz, Brian; Kachmar, Brian A.

    2006-01-01

    NASA s Aviation Safety Program was created for the purpose of making a significant reduction in the incidents of weather related aviation accidents by improving situational awareness. The objectives of that program are being met in part through advances in weather sensor technology, and in part through advances in the communications technology that are developed for use in the National Airspace System. It is this latter element, i.e., the improvements in aviation communication technologies, that is the focus of the Weather Information Communications project. This report describes the final flight test results completed under the WINCOMM project at the NASA Glenn Research Center of the 1090 Extended Squitter (1090ES) and VDL Mode 3 (VDL-3) data links as a medium for weather data exchange. It presents the use of 1090ES to meet the program objectives of sending broadcast turbulence information and the use of VDL-3 to send graphical weather images. This report provides the test requirements and test plans, which led to flight tests, as well as final results from flight testing. The reports define the changes made to both avionics and ground-based receivers as well as the ground infrastructure to support implementation of the recommended architecture, with a focus on the issues associated with these changes.

  4. Prevalence of Overweight and Obesity among Primary School Children Aged 8–13 Years in Dar es Salaam City, Tanzania

    PubMed Central

    Pangani, Ismail N.; Kiplamai, Festus K.; Kamau, Jane W.; Onywera, Vincent O.

    2016-01-01

    Background. The understanding of obesity as a growing health problem in Africa and Tanzania in particular is hampered by lack of data as well as sociocultural beliefs in which overweight and obesity are revered. This study sought to determine the prevalence of overweight and obesity among primary school children aged 8–13 years in Dar es Salaam, Tanzania. Method. A cross-sectional analytical research design was used to study overweight and obesity in primary schools in Dar es Salaam, Tanzania. The target population was 150,000 children aged 8–13 years. Stratified random sampling was used to select 1781 children. Weight and height were taken and WHO standards for children were used to determine weight status. Results. Findings showed that the prevalence of overweight and obesity was 15.9% and 6.7%, respectively (N = 1781). However, 6.2% of the children were underweight. There were significant differences in mean BMI between children in private and public schools (p = 0.021), between male and female (p < 0.001), and across age groups of 8–10 and 11–13 years (p < 0.001). Conclusion. The prevalence of overweight and obesity among primary school children is significant and requires management and prevention strategies. PMID:27403343

  5. MAGIC discovery of the BL Lac 1ES 1727+502: Multiwavelength observations, spectral behavior and variability

    NASA Astrophysics Data System (ADS)

    De Caneva, G.; Berger, K.; Lindfors, E.; Lombardi, S.; Mankuzhiyil, N.; Paneque, D.; Stamerra, A.; Tavecchio, F.; MAGIC Collaboration; Buson, S.; Fermi-LAT Collaboration

    2012-12-01

    The MAGIC experiment is a system of two Imaging Atmospheric Cherenkov Telescopes located in the Canary Island of La Palma (Northern hemisphere). It has an energy threshold of 50 GeV, the lowest among the currently operating Cherenkov telescopes, which makes it particularly suitable for the observation of extragalactic sources at Very High Energies (VHE, E>100 GeV). MAGIC has detected numerous blazars, which are active galactic nuclei whose jet axis is pointed towards the observer. Here we present one of our latest detections, the BL Lac 1ES 1727+502, located at redshift z=0.055. The source was a promising TeV candidate based on archival data and the observation that leads this detection was not triggered by any high state alert in other wavebands. We complemented our data with multiwavelength observations: optical data from the KVA telescope, UV, optical and X-ray data taken with the instruments on board the Swift satellite and High Energy (HE, 100; MeV < E < 100; GeV) data from the Fermi-LAT (Large Area Telescope). We studied the spectral energy distribution (SED) of 1ES 1727+502 and interpreted it with a one-zone synchrotron self-Compton model obtaining parameters typical for this class of sources.

  6. Prevalence of Overweight and Obesity among Primary School Children Aged 8-13 Years in Dar es Salaam City, Tanzania.

    PubMed

    Pangani, Ismail N; Kiplamai, Festus K; Kamau, Jane W; Onywera, Vincent O

    2016-01-01

    Background. The understanding of obesity as a growing health problem in Africa and Tanzania in particular is hampered by lack of data as well as sociocultural beliefs in which overweight and obesity are revered. This study sought to determine the prevalence of overweight and obesity among primary school children aged 8-13 years in Dar es Salaam, Tanzania. Method. A cross-sectional analytical research design was used to study overweight and obesity in primary schools in Dar es Salaam, Tanzania. The target population was 150,000 children aged 8-13 years. Stratified random sampling was used to select 1781 children. Weight and height were taken and WHO standards for children were used to determine weight status. Results. Findings showed that the prevalence of overweight and obesity was 15.9% and 6.7%, respectively (N = 1781). However, 6.2% of the children were underweight. There were significant differences in mean BMI between children in private and public schools (p = 0.021), between male and female (p < 0.001), and across age groups of 8-10 and 11-13 years (p < 0.001). Conclusion. The prevalence of overweight and obesity among primary school children is significant and requires management and prevention strategies. PMID:27403343

  7. The hadronic origin of the hard gamma-ray spectrum from blazar 1ES 1101-232

    SciTech Connect

    Cao, Gang; Wang, Jiancheng E-mail: jcwang@ynao.ac.cn

    2014-03-10

    The very hard γ-ray spectrum from distant blazars challenges the traditional synchrotron self-Compton (SSC) model, which may indicate that there is a contribution from an additional high-energy component beyond the SSC emission. In this paper, we study the possible origin of the hard γ-ray spectrum from distant blazars. We develop a model to explain the hard γ-ray spectrum from blazar 1ES 1101-232. In the model, the optical and X-ray radiation would come from the synchrotron radiation of primary electrons and secondary pairs and the GeV emission would be produced by the SSC process, however, the hard γ-ray spectrum would originate from the decay of neutral pion produced through proton-photon interactions with the synchrotron radiation photons within the jet. Our model can explain the observed spectral energy distribution of 1ES 1101-232 well, especially the very hard γ-ray spectrum. However, our model requires a very large proton power to efficiently produce the γ-ray through proton-photon interactions.

  8. A Wearable EEG-HEG-HRV Multimodal System With Simultaneous Monitoring of tES for Mental Health Management.

    PubMed

    Ha, Unsoo; Lee, Yongsu; Kim, Hyunki; Roh, Taehwan; Bae, Joonsung; Kim, Changhyeon; Yoo, Hoi-Jun

    2015-12-01

    A multimodal mental management system in the shape of the wearable headband and earplugs is proposed to monitor electroencephalography (EEG), hemoencephalography (HEG) and heart rate variability (HRV) for accurate mental health monitoring. It enables simultaneous transcranial electrical stimulation (tES) together with real-time monitoring. The total weight of the proposed system is less than 200 g. The multi-loop low-noise amplifier (MLLNA) achieves over 130 dB CMRR for EEG sensing and the capacitive correlated-double sampling transimpedance amplifier (CCTIA) has low-noise characteristics for HEG and HRV sensing. Measured three-physiology domains such as neural, vascular and autonomic domain signals are combined with canonical correlation analysis (CCA) and temporal kernel canonical correlation analysis (tkCCA) algorithm to find the neural-vascular-autonomic coupling. It supports highly accurate classification with the 19% maximum improvement with multimodal monitoring. For the multi-channel stimulation functionality, after-effects maximization monitoring and sympathetic nerve disorder monitoring, the stimulator is designed as reconfigurable. The 3.37 × 2.25 mm(2) chip has 2-channel EEG sensor front-end, 2-channel NIRS sensor front-end, NIRS current driver to drive dual-wavelength VCSEL and 6-b DAC current source for tES mode. It dissipates 24 mW with 2 mA stimulation current and 5 mA NIRS driver current.

  9. Bacillus pumilus ES4: candidate plant growth-promoting bacterium to enhance establishment of plants in mine tailings

    PubMed Central

    de-Bashan, Luz E.; Hernandez, Juan-Pablo; Bashan, Yoav; Maier, Raina

    2014-01-01

    Three plant growth-promoting bacteria (PGPB; Bacillus pumilus ES4, B. pumilus RIZO1, and Azospirillum brasilense Cd) were tested for their ability to enhance plant growth and development of the native Sonoran Desert shrub quailbush (Atriplex lentiformis) and for their effect on the native bacterial community in moderately acidic, high-metal content (AHMT) and in neutral, low metal content natural tailings (NLMT) in controlled greenhouse experiments. Inoculation of quailbush with all three PGPB significantly enhanced plant growth parameters, such as germination, root length, dry weight of shoots and roots, and root/shoot ratio in both types of tailings. The effect of inoculation on the indigenous bacterial community by the most successful PGPB Bacillus pumilus ES4 was evaluated by denaturating gradient gel electrophoresis (PCR-DGGE) fingerprinting and root colonization was followed by specific fluorescent in situ hybridization (FISH). Inoculation with this strain significantly changed the bacterial community over a period of 60 days. FISH analysis showed that the preferred site of colonization was the root tips and root elongation area. This study shows that inoculation of native perennial plants with PGPB can be used for developing technologies for phytostabilizing mine tailings. PMID:25009362

  10. Coping with urban growth and development through environmental planning and management (EPM): the sustainable Dar es Salaam project.

    PubMed

    Majani, B B

    1996-03-01

    This article discusses the Urban Management Program (UMP) in Dar es Salaam, Tanzania. The UMP is a joint effort of UNCHS (Habitat), UNDP, and the World Bank. The UMP established the Sustainable Cities Program (SCP) in August 1990. The aim was to provide city officials and their partners in private, public, and popular sectors with improved environmental planning and management capacity. Dar es Salaam has 4 major land formations that constrain management options. About 70% of urban population live in unplanned areas with marginal access to piped water, sanitation, drainage, or basic social services. Improper waste disposal has contributed to water pollution. Under 3% of the city's solid waste is collected. Low lying areas along the coast become flooded, and poor drainage causes continually flooded road systems. SCP began a 4-stage process to identify issues, develop and implement strategy and action plans, and institutionalize the process. An environmental profile was established in 1992. Plans were developed for solid waste management, upgrading unplanned settlements, servicing planned land and city center renewal, and managing open spaces, wastes, and petty trading. These efforts were institutionalized. The efforts are noteworthy for their active participation in plan preparation by key urban managers, multisectoral coordination on environmental issues, involvement of the private sector, establishment of priorities, and detailed action plans. Political support at the highest levels combined with community participation were key to program success. The lessons learned are identified.

  11. Exploring the association between women's access to economic resources and intimate partner violence in Dar es Salaam and Mbeya, Tanzania.

    PubMed

    Vyas, Seema; Jansen, Henrica Afm; Heise, Lori; Mbwambo, Jessie

    2015-12-01

    The relationship between women's access to economic resources, e.g. employment or access to micro-credit, and experience of intimate partner violence is complex. Empirical evidence documents that in some settings women's employment is associated with higher risk of partner violence but in other settings with lower risk. Evidence also shows that these conflicting associations exist not only between countries but also within different country settings. Using two population-based data sets gathered in 2002 in contrasting Tanzania settings-Dar es Salaam and Mbeya-, we used multivariate logistic regression to examine the relationship between women's access to economic resources and partner violence. Two indicators of economic resources were examined: whether women earned money and whether women owned a business either with someone or exclusively. In Dar es Salaam we found evidence of a higher risk association among women who earned money and who owned a business exclusively by themselves and a lower risk association among women who owned a business with someone. We found no relationship between either indicator of economic resources and partner violence in Mbeya. Other factors were similarly associated with partner violence in both settings and the strongest associations found were related to the respondents' partners: refusal to give money; alcohol use and relationships with other women. The findings support the assertion that women's access to economic resources operate differently in different country settings, thus highlighting the need for targeted prevention efforts that are relevant for the context.

  12. METHOTREXATE AND MYOTREXATE INDUCE APOPTOSIS IN HUMAN MYOMA FIBROBLASTS (T hES CELL LINE) VIA MITOCHONDRIAL PATHWAY.

    PubMed

    Kastratović, Tatjana; Arsenijević, Slobodan; Matović, Zoran; Mitrović, Marina; Nikolić, Ivana; Milosavljević, Zoran; Protrka, Zoran; Šorak, Marija; Đurić, Janko

    2015-01-01

    Uterine leiomyomas (fibroids) are the most common benign tumors in women of reproductive age. Although the local application of low doses of methotrexate (MTX) is used as an effective treatment of the myomas, myotrexate could be a promising new drug. This study investigated the cytotoxic and apoptotic effects of both MTX and myotrexate in human fibroblasts derived from the uterine fibroids (T hES cell line). The myotrexate adduct is an aqueous solution of MTX and L-arginine. Cells were treated with a graded concentrations of both MTX and myothrexate (0.1-16 µM) for 24 h. The cytotoxicity was assayed by MTT test, apoptosis was evaluated by Annexin V-FITC assay and their possible role in apoptosis was determined by immnu- flourescence. Both MTX and myotrexate induced apoptosis in T hES cells in a dose dependent manner (p < 0.001). Myotrexate significantly increased the percentage of AnnexinV positive cells, BAX/Bcl-2 ratio and subsequent caspase-3 activation compared to the MTX treated cells (p < 0.05). Both MTX or myotrexate treatment showed a diffuse staining of cytochrome c indicating its release from mitochondria to the cytosol, suggesting that their mechanisms of action most likely involves the mitochondrial apoptotic pathway. PMID:26642654

  13. Exploring the association between women's access to economic resources and intimate partner violence in Dar es Salaam and Mbeya, Tanzania.

    PubMed

    Vyas, Seema; Jansen, Henrica Afm; Heise, Lori; Mbwambo, Jessie

    2015-12-01

    The relationship between women's access to economic resources, e.g. employment or access to micro-credit, and experience of intimate partner violence is complex. Empirical evidence documents that in some settings women's employment is associated with higher risk of partner violence but in other settings with lower risk. Evidence also shows that these conflicting associations exist not only between countries but also within different country settings. Using two population-based data sets gathered in 2002 in contrasting Tanzania settings-Dar es Salaam and Mbeya-, we used multivariate logistic regression to examine the relationship between women's access to economic resources and partner violence. Two indicators of economic resources were examined: whether women earned money and whether women owned a business either with someone or exclusively. In Dar es Salaam we found evidence of a higher risk association among women who earned money and who owned a business exclusively by themselves and a lower risk association among women who owned a business with someone. We found no relationship between either indicator of economic resources and partner violence in Mbeya. Other factors were similarly associated with partner violence in both settings and the strongest associations found were related to the respondents' partners: refusal to give money; alcohol use and relationships with other women. The findings support the assertion that women's access to economic resources operate differently in different country settings, thus highlighting the need for targeted prevention efforts that are relevant for the context. PMID:26494417

  14. Measurement and analysis of the neutron-induced fission cross sections of {sup 247}Cm, {sup 250}Cr and {sup 254}Es

    SciTech Connect

    Danon, Y.; Moore, M.S.; Koehler, P.E.; Lougheed, R.W.; Hoff, R.W.; Hill, N.W.

    1994-05-01

    A series of fission cross section measurements were performed on {sup 247}Cm, {sup 250}Cf and {sup 254}Es. This paper summarizes the most recent results and details the resonance parameter analysis done on {sup 247}Cm.

  15. Revisiting the GroEL-GroES Reaction Cycle via the Symmetric Intermediate Implied by Novel Aspects of the GroEL(D398A) Mutant*♦

    PubMed Central

    Koike-Takeshita, Ayumi; Yoshida, Masasuke; Taguchi, Hideki

    2008-01-01

    The Escherichia coli chaperonin GroEL is a double-ring chaperone that assists in protein folding with the aid of GroES and ATP. It is believed that GroEL alternates the folding-active rings and that the substrate protein (and GroES) can bind to the open trans-ring only after ATP in the cis-ring is hydrolyzed. However, we found that a substrate protein prebound to the trans-ring remained bound during the first ATP cycle, and this substrate was assisted by GroEL-GroES when the second cycle began. Moreover, a slow ATP-hydrolyzing GroEL mutant (D398A) in the ATP-bound form bound a substrate protein and GroES to the trans-ring. The apparent discrepancy with the results from an earlier study (Rye, H. S., Roseman, A. M., Chen, S., Furtak, K., Fenton, W. A., Saibil, H. R., and Horwich, A. L. (1999) Cell 97, 325–338) can be explained by the previously unnoticed fact that the ATP-bound form of the D398A mutant exists as a symmetric 1:2 GroEL-GroES complex (the “football”-shaped complex) and that the substrate protein (and GroES) in the medium is incorporated into the complex only after the slow turnover. In light of these results, the current model of the GroEL-GroES reaction cycle via the asymmetric 1:1 GroEL-GroES complex deserves reexamination. PMID:18567584

  16. c-Yes tyrosine kinase is a potent suppressor of ES cell differentiation and antagonizes the actions of its closest phylogenetic relative, c-Src.

    PubMed

    Zhang, Xiong; Meyn, Malcolm A; Smithgall, Thomas E

    2014-01-17

    Embryonic stem (ES) cells are derived from the inner cell mass of the blastocyst stage embryo and are characterized by self-renewal and pluripotency. Previous work has shown that Src-family tyrosine kinases display dynamic expression and activity changes during ES cell differentiation, suggesting distinct functions in the control of developmental fate. Here we used ES cells to test the hypothesis that c-Src and its closest phylogenetic relative, c-Yes, act in biological opposition despite their strong homology. Unlike c-Src, enforced expression of active c-Yes blocked ES cell differentiation to embryoid bodies by maintaining pluripotency gene expression. To explore the interplay of c-Src and c-Yes in ES cell differentiation, we engineered c-Src and c-Yes mutants that are resistant to A-419259, a potent pyrrolopyrimidine inhibitor of the Src kinase family. Previous studies have shown that A-419259 treatment blocks all Src-family kinase activity in ES cells, preventing differentiation while maintaining pluripotency. Expression of inhibitor-resistant c-Src but not c-Yes rescued the A-419259 differentiation block, resulting in a cell population with properties of both primitive ectoderm and endoderm. Remarkably, when inhibitor-resistant c-Src and c-Yes were expressed together in ES cells, c-Yes activity suppressed c-Src-mediated differentiation. These studies show that even closely related kinases such as c-Src and c-Yes have unique and opposing functions in the same cell type. Selective agonists or inhibitors of c-Src versus c-Yes activity may allow more precise pharmacological manipulation of ES cell fate and have broader applications in other biological systems that express multiple Src family members such as tumor cells.

  17. Differential conformational modulations of MreB folding upon interactions with GroEL/ES and TRiC chaperonin components

    PubMed Central

    Moparthi, Satish Babu; Carlsson, Uno; Vincentelli, Renaud; Jonsson, Bengt-Harald; Hammarström, Per; Wenger, Jérôme

    2016-01-01

    Here, we study and compare the mechanisms of action of the GroEL/GroES and the TRiC chaperonin systems on MreB client protein variants extracted from E. coli. MreB is a homologue to actin in prokaryotes. Single-molecule fluorescence correlation spectroscopy (FCS) and time-resolved fluorescence polarization anisotropy report the binding interaction of folding MreB with GroEL, GroES and TRiC. Fluorescence resonance energy transfer (FRET) measurements on MreB variants quantified molecular distance changes occurring during conformational rearrangements within folding MreB bound to chaperonins. We observed that the MreB structure is rearranged by a binding-induced expansion mechanism in TRiC, GroEL and GroES. These results are quantitatively comparable to the structural rearrangements found during the interaction of β-actin with GroEL and TRiC, indicating that the mechanism of chaperonins is conserved during evolution. The chaperonin-bound MreB is also significantly compacted after addition of AMP-PNP for both the GroEL/ES and TRiC systems. Most importantly, our results showed that GroES may act as an unfoldase by inducing a dramatic initial expansion of MreB (even more than for GroEL) implicating a role for MreB folding, allowing us to suggest a delivery mechanism for GroES to GroEL in prokaryotes. PMID:27328749

  18. Permeable Reactive Biobarriers for In Situ Cr(VI) Reduction: Bench Scale Tests Using Cellulomonas sp. Strain ES6

    SciTech Connect

    Sridhar Viamajala; Brent M. Peyton; Robin Gerlach; Vaideeswaran; William A. Apel; James N. Petersen

    2008-12-01

    Chromate (Cr(VI)) reduction studies were performed in bench scale flow columns using the fermentative subsurface isolate Cellulomonas sp. strain ES6. In these tests, columns packed with either quartz sand or hydrous ferric oxide (HFO)-coated quartz sand, were inoculated with strain ES6 and fed nutrients to stimulate growth before nutrient-free Cr(VI) solutions were injected. Results show that in columns containing quartz sand, a continuous inflow of 2 mg/L Cr(VI) was reduced to below detection limits in the effluent for durations of up to 5.7 residence times after nutrient injection was discontinued proving the ability of strain ES6 to reduce chromate in the absence of an external electron donor. In the HFO-containing columns, Cr(VI) reduction was significantly prolonged and effluent Cr(VI) concentrations remained below detectable levels for periods of up to 66 residence times after nutrient injection was discontinued. Fe was detected in the effluent of the HFO-containing columns throughout the period of Cr(VI) removal indicating that the insoluble Fe(III) bearing solids were being continuously reduced to form soluble Fe(II) resulting in prolonged abiotic Cr(VI) reduction. Thus, growth of Cellulomonas within the soil columns resulted in formation of permeable reactive barriers that could reduce Cr(VI) and Fe(III) for extended periods even in the absence of external electron donors. Other bioremediation systems employing Fe(II)-mediated reactions require a continuous presence of external nutrients to regenerate Fe(II). After depletion of nutrients, contaminant removal within these systems occurs by reaction with surface-associated Fe(II) that can rapidly become inaccessible due to formation of crystalline Fe-minerals or other precipitates. The ability of fermentative organisms like Cellulomonas to reduce metals without continuous nutrient supply in the subsurface offers a viable and economical alternative technology for in situ remediation of Cr

  19. Permeable reactive biobarriers for in situ Cr(VI) reduction: bench scale tests using Cellulomonas sp. strain ES6.

    PubMed

    Viamajala, Sridhar; Peyton, Brent M; Gerlach, Robin; Sivaswamy, Vaideeswaran; Apel, William A; Petersen, James N

    2008-12-15

    Chromate (Cr(VI)) reduction studies were performed in bench scale flow columns using the fermentative subsurface isolate Cellulomonas sp. strain ES6. In these tests, columns packed with either quartz sand or hydrous ferric oxide (HFO)-coated quartz sand, were inoculated with strain ES6 and fed nutrients to stimulate growth before nutrient-free Cr(VI) solutions were injected. Results show that in columns containing quartz sand, a continuous inflow of 2 mg/L Cr(VI) was reduced to below detection limits in the effluent for durations of up to 5.7 residence times after nutrient injection was discontinued proving the ability of strain ES6 to reduce chromate in the absence of an external electron donor. In the HFO-containing columns, Cr(VI) reduction was significantly prolonged and effluent Cr(VI) concentrations remained below detectable levels for periods of up to 66 residence times after nutrient injection was discontinued. Fe was detected in the effluent of the HFO-containing columns throughout the period of Cr(VI) removal indicating that the insoluble Fe(III) bearing solids were being continuously reduced to form soluble Fe(II) resulting in prolonged abiotic Cr(VI) reduction. Thus, growth of Cellulomonas within the soil columns resulted in formation of permeable reactive barriers that could reduce Cr(VI) and Fe(III) for extended periods even in the absence of external electron donors. Other bioremediation systems employing Fe(II)-mediated reactions require a continuous presence of external nutrients to regenerate Fe(II). After depletion of nutrients, contaminant removal within these systems occurs by reaction with surface-associated Fe(II) that can rapidly become inaccessible due to formation of crystalline Fe-minerals or other precipitates. The ability of fermentative organisms like Cellulomonas to reduce metals without continuous nutrient supply in the subsurface offers a viable and economical alternative technology for in situ remediation of Cr

  20. HUBBLE/COS OBSERVATIONS OF THE Ly{alpha} FOREST TOWARD THE BL Lac OBJECT 1ES 1553+113

    SciTech Connect

    Danforth, Charles W.; Keeney, Brian A.; Stocke, John T.; Shull, J. Michael; Yao Yangsen

    2010-09-01

    We present new moderate-resolution, far-ultraviolet spectra from the Hubble Space Telescope/Cosmic Origins Spectrograph (HST/COS) of the BL Lac object 1ES 1553+113 covering the wavelength range 1135 A < {lambda} < 1795 A. The data show a smooth continuum with a wealth of narrow (b < 100 km s{sup -1}) absorption features arising in the interstellar medium and intergalactic medium. These features include 41 Ly{alpha} absorbers at 0 < z{sub abs} < 0.43, 14 of which are detected in multiple Lyman lines and 6 of which show absorption in one or more metal lines. We analyze a metal-rich triplet ({Delta}cz {approx} 1000 km s{sup -1}) of Ly{alpha} absorbers at z{sub abs} {approx} 0.188 in which O VI, N V, and C III absorption is detected. Silicon ions (Si III, Si IV) are not detected to fairly strong upper limits and we use the measured Si III/C III upper limit to derive an abundance limit (C/Si) {>=} 4(C/Si){sub sun} for the strongest component of the absorber complex. Galaxy redshift surveys show a number of massive galaxies at approximately the same redshift as this absorption complex, suggesting that it arises in a large-scale galaxy filament. As one of the brightest extragalactic X-ray and {gamma}-ray sources, 1ES 1553+113 is of great interest to the high-energy astrophysics community. With no intrinsic emission or absorption features, 1ES 1553+113 has no direct redshift determination. We use intervening Ly{alpha} absorbers to place a direct limit on the redshift: z{sub em}>0.395 based on a confirmed Ly{alpha}+O VI absorber and z{sub em}>0.433 based on a single-line detection of Ly{alpha}. The current COS data are only sensitive to Ly{alpha} absorbers at z < 0.47, but we present statistical arguments that z{sub em} {approx}< 0.58 (at a 1{sigma} confidence limit) based on the non-detection of any Ly{beta} absorbers at z>0.4.

  1. The New York Head-A precise standardized volume conductor model for EEG source localization and tES targeting.

    PubMed

    Huang, Yu; Parra, Lucas C; Haufe, Stefan

    2016-10-15

    In source localization of electroencephalograpic (EEG) signals, as well as in targeted transcranial electric current stimulation (tES), a volume conductor model is required to describe the flow of electric currents in the head. Boundary element models (BEM) can be readily computed to represent major tissue compartments, but cannot encode detailed anatomical information within compartments. Finite element models (FEM) can capture more tissue types and intricate anatomical structures, but with the higher precision also comes the need for semi-automated segmentation, and a higher computational cost. In either case, adjusting to the individual human anatomy requires costly magnetic resonance imaging (MRI), and thus head modeling is often based on the anatomy of an 'arbitrary' individual (e.g. Colin27). Additionally, existing reference models for the human head often do not include the cerebro-spinal fluid (CSF), and their field of view excludes portions of the head and neck-two factors that demonstrably affect current-flow patterns. Here we present a highly detailed FEM, which we call ICBM-NY, or "New York Head". It is based on the ICBM152 anatomical template (a non-linear average of the MRI of 152 adult human brains) defined in MNI coordinates, for which we extended the field of view to the neck and performed a detailed segmentation of six tissue types (scalp, skull, CSF, gray matter, white matter, air cavities) at 0.5mm(3) resolution. The model was solved for 231 electrode locations. To evaluate its performance, additional FEMs and BEMs were constructed for four individual subjects. Each of the four individual FEMs (regarded as the 'ground truth') is compared to its BEM counterpart, the ICBM-NY, a BEM of the ICBM anatomy, an 'individualized' BEM of the ICBM anatomy warped to the individual head surface, and FEMs of the other individuals. Performance is measured in terms of EEG source localization and tES targeting errors. Results show that the ICBM-NY outperforms

  2. Insights into the emission of the blazar 1ES 1011+496 through unprecedented broadband observations during 2011 and 2012

    NASA Astrophysics Data System (ADS)

    Aleksić, J.; Ansoldi, S.; Antonelli, L. A.; Antoranz, P.; Arcaro, C.; Babic, A.; Bangale, P.; Barres de Almeida, U.; Barrio, J. A.; Becerra González, J.; Bednarek, W.; Bernardini, E.; Biasuzzi, B.; Biland, A.; Blanch, O.; Bonnefoy, S.; Bonnoli, G.; Borracci, F.; Bretz, T.; Carmona, E.; Carosi, A.; Colin, P.; Colombo, E.; Contreras, J. L.; Cortina, J.; Covino, S.; Da Vela, P.; Dazzi, F.; De Angelis, A.; De Caneva, G.; De Lotto, B.; de Oña Wilhelmi, E.; Delgado Mendez, C.; Di Pierro, F.; Dominis Prester, D.; Dorner, D.; Doro, M.; Einecke, S.; Eisenacher, D.; Elsaesser, D.; Fernández-Barral, A.; Fidalgo, D.; Fonseca, M. V.; Font, L.; Frantzen, K.; Fruck, C.; Galindo, D.; García López, R. J.; Garczarczyk, M.; Garrido Terrats, D.; Gaug, M.; Godinović, N.; González Muñoz, A.; Gozzini, S. R.; Hadasch, D.; Hanabata, Y.; Hayashida, M.; Herrera, J.; Hose, J.; Hrupec, D.; Idec, W.; Kadenius, V.; Kellermann, H.; Knoetig, M. L.; Kodani, K.; Konno, Y.; Krause, J.; Kubo, H.; Kushida, J.; La Barbera, A.; Lelas, D.; Lewandowska, N.; Lindfors, E.; Lombardi, S.; Longo, F.; López, M.; López-Coto, R.; López-Oramas, A.; Lorenz, E.; Lozano, I.; Makariev, M.; Mallot, K.; Maneva, G.; Mannheim, K.; Maraschi, L.; Marcote, B.; Mariotti, M.; Martínez, M.; Mazin, D.; Menzel, U.; Miranda, J. M.; Mirzoyan, R.; Moralejo, A.; Munar-Adrover, P.; Nakajima, D.; Neustroev, V.; Niedzwiecki, A.; Nievas Rosillo, M.; Nilsson, K.; Nishijima, K.; Noda, K.; Orito, R.; Overkemping, A.; Paiano, S.; Palatiello, M.; Paneque, D.; Paoletti, R.; Paredes, J. M.; Paredes-Fortuny, X.; Persic, M.; Poutanen, J.; Prada Moroni, P. G.; Prandini, E.; Puljak, I.; Reinthal, R.; Rhode, W.; Ribó, M.; Rico, J.; Rodriguez Garcia, J.; Saito, T.; Saito, K.; Satalecka, K.; Scalzotto, V.; Scapin, V.; Schweizer, T.; Shore, S. N.; Sillanpää, A.; Sitarek, J.; Snidaric, I.; Sobczynska, D.; Stamerra, A.; Steinbring, T.; Strzys, M.; Takalo, L.; Takami, H.; Tavecchio, F.; Temnikov, P.; Terzić, T.; Tescaro, D.; Teshima, M.; Thaele, J.; Torres, D. F.; Toyama, T.; Treves, A.; Vogler, P.; Will, M.; Zanin, R.; Buson, S.; D'Ammando, F.; Lähteenmäki, A.; Hovatta, T.; Kovalev, Y. Y.; Lister, M. L.; Max-Moerbeck, W.; Mundell, C.; Pushkarev, A. B.; Rastorgueva-Foi, E.; Readhead, A. C. S.; Richards, J. L.; Tammi, J.; Sanchez, D. A.; Tornikoski, M.; Savolainen, T.; Steele, I.

    2016-06-01

    Context. 1ES 1011+496 (z = 0.212) was discovered in very high-energy (VHE, E> 100 GeV) γ rays with MAGIC in 2007. The absence of simultaneous data at lower energies led to an incomplete characterization of the broadband spectral energy distribution (SED). Aims: We study the source properties and the emission mechanisms, probing whether a simple one-zone synchrotron self-Compton (SSC) scenario is able to explain the observed broadband spectrum. Methods: We analyzed data in the range from VHE to radio data from 2011 and 2012 collected by MAGIC, Fermi-LAT, Swift, KVA, OVRO, and Metsähovi in addition to optical polarimetry data and radio maps from the Liverpool Telescope and MOJAVE. Results: The VHE spectrum was fit with a simple power law with a photon index of 3.69 ± 0.22 and a flux above 150 GeV of (1.46 ± 0.16) × 10-11 ph cm-2 s-1. The source 1ES 1011+496 was found to be in a generally quiescent state at all observed wavelengths, showing only moderate variability from radio to X-rays. A low degree of polarization of less than 10% was measured in optical, while some bright features polarized up to 60% were observed in the radio jet. A similar trend in the rotation of the electric vector position angle was found in optical and radio. The radio maps indicated a superluminal motion of 1.8 ± 0.4 c, which is the highest speed statistically significant measured so far in a high-frequency-peaked BL Lac. Conclusions: For the first time, the high-energy bump in the broadband SED of 1ES 1011+496 could be fully characterized from 0.1 GeV to 1 TeV, which permitted a more reliable interpretation within the one-zone SSC scenario. The polarimetry data suggest that at least part of the optical emission has its origin in some of the bright radio features, while the low polarization in optical might be due to the contribution of parts of the radio jet with different orientations of the magnetic field with respect to the optical emission.

  3. E/S0 GALAXIES ON THE BLUE COLOR-STELLAR MASS SEQUENCE AT z = 0: FADING MERGERS OR FUTURE SPIRALS?

    SciTech Connect

    Kannappan, Sheila J.; Guie, Jocelly M.; Baker, Andrew J. E-mail: jocelly@mail.utexas.edu

    2009-08-15

    We identify a population of morphologically defined E/S0 galaxies lying on the locus of late-type galaxies in color-stellar mass space - the 'blue sequence' -at the present epoch. Using three samples (from the Nearby Field Galaxy Survey or NFGS, a merged HyperLeda/Sloan Digital Sky Survey/Two Micron All Sky Survey catalog, and the NYU Value-Added Galaxy Catalog), we analyze blue-sequence E/S0s with stellar masses {approx}>10{sup 8} M {sub sun}, arguing that individual objects may be evolving either up toward the red sequence or down into the blue sequence. Blue-sequence E/S0 galaxies become more common with decreasing stellar mass, comprising {approx}<2% of E/S0s near the 'shutdown mass' M{sub s} {approx} 1-2 x 10{sup 11} M {sub sun}, increasing to {approx}>5% near the 'bimodality mass' M{sub b} {approx} 3 x 10{sup 10} M {sub sun}, and sharply rising to {approx}> 20%-30% below the 'threshold mass' M{sub t} {approx} 4-6 x 10{sup 9} M {sub sun}, down to our completeness analysis limit at {approx}10{sup 9} M {sub sun}. The strong emergence of blue-sequence E/S0s below M{sub t} coincides with a previously reported global increase in mean atomic gas fractions below M{sub t} for galaxies of all types on both sequences, suggesting that the availability of cold gas may be basic to blue-sequence E/S0s' existence. Environmental analysis reveals that many sub-M{sub b} blue-sequence E/S0s reside in low-to-intermediate density environments. Thus, the bulk of the population we analyze appears distinct from the generally lower-mass cluster dE population; S0 morphologies with a range of bulge sizes are typical. In mass-radius and mass-{sigma} scaling relations, blue-sequence E/S0s are more similar to red-sequence E/S0s than to late-type galaxies, but they represent a transitional class. While some of them, especially in the high-mass range from M{sub b} to M{sub s} , resemble major-merger remnants that will likely fade onto the red sequence, most blue-sequence E/S0s below M{sub b

  4. The Risk of Dengue Virus Transmission in Dar es Salaam, Tanzania during an Epidemic Period of 2014

    PubMed Central

    Mboera, Leonard E. G.; Mweya, Clement N.; Rumisha, Susan F.; Tungu, Patrick K.; Stanley, Grades; Makange, Mariam R.; Misinzo, Gerald; De Nardo, Pasquale; Vairo, Francesco; Oriyo, Ndekya M.

    2016-01-01

    Background In 2010, 2012, 2013 and 2014 dengue outbreaks have been reported in Dar es Salaam, Tanzania. However, there is no comprehensive data on the risk of transmission of dengue in the country. The objective of this study was to assess the risk of transmission of dengue in Dar es Salaam during the 2014 epidemic. Methodology/Principal Findings This cross-sectional study was conducted in Dar es Salaam, Tanzania during the dengue outbreak of 2014. The study involved Ilala, Kinondoni and Temeke districts. Adult mosquitoes were collected using carbon dioxide-propane powered Mosquito Magnet Liberty Plus traps. In each household compound, water-holding containers were examined for mosquito larvae and pupae. Dengue virus infection of mosquitoes was determined using real-time reverse transcription polymerase chain reaction (qRT-PCR). Partial amplification and sequencing of dengue virus genome in infected mosquitoes was performed. A total of 1,000 adult mosquitoes were collected. Over half (59.9%) of the adult mosquitoes were collected in Kinondoni. Aedes aegypti accounted for 17.2% of the mosquitoes of which 90.6% were from Kinondoni. Of a total of 796 houses inspected, 38.3% had water-holding containers in their premises. Kinondoni had the largest proportion of water-holding containers (57.7%), followed by Temeke (31.4%) and Ilala (23.4%). The most common breeding containers for the Aedes mosquitoes were discarded plastic containers and tires. High Aedes infestation indices were observed for all districts and sites, with a house index of 18.1% in Ilala, 25.5% in Temeke and 35.3% in Kinondoni. The respective container indices were 77.4%, 65.2% and 80.2%. Of the reared larvae and pupae, 5,250 adult mosquitoes emerged, of which 61.9% were Ae. aegypti. Overall, 27 (8.18) of the 330 pools of Ae. aegypti were positive for dengue virus. On average, the overall maximum likelihood estimate (MLE) indicates pooled infection rate of 8.49 per 1,000 mosquitoes (95%CI = 5.72–12

  5. Oligo- and Polymetastatic Progression in Lung Metastasis(es) Patients Is Associated with Specific MicroRNAs

    PubMed Central

    Lussier, Yves A.; Ganai, Sabha; Khan, Sajid A.; Gnerlich, Jennifer; Darga, Thomas E.; Fan, Hanli; Karpenko, Oleksiy; Paty, Philip B.; Posner, Mitchell C.; Chmura, Steven J.; Hellman, Samuel; Ferguson, Mark K.; Weichselbaum, Ralph R.

    2012-01-01

    Rationale Strategies to stage and treat cancer rely on a presumption of either localized or widespread metastatic disease. An intermediate state of metastasis termed oligometastasis(es) characterized by limited progression has been proposed. Oligometastases are amenable to treatment by surgical resection or radiotherapy. Methods We analyzed microRNA expression patterns from lung metastasis samples of patients with ≤5 initial metastases resected with curative intent. Results Patients were stratified into subgroups based on their rate of metastatic progression. We prioritized microRNAs between patients with the highest and lowest rates of recurrence. We designated these as high rate of progression (HRP) and low rate of progression (LRP); the latter group included patients with no recurrences. The prioritized microRNAs distinguished HRP from LRP and were associated with rate of metastatic progression and survival in an independent validation dataset. Conclusion Oligo- and poly- metastasis are distinct entities at the clinical and molecular level. PMID:23251360

  6. Multiwavelength Observations of the AGN 1ES 0414+009 with VERITAS, Fermi-LAT, Swift-XRT, and MDM

    NASA Astrophysics Data System (ADS)

    Aliu, E.; Archambault, S.; Arlen, T.; Aune, T.; Beilicke, M.; Benbow, W.; Böttcher, M.; Bouvier, A.; Bugaev, V.; Cannon, A.; Cesarini, A.; Ciupik, L.; Collins-Hughes, E.; Connolly, M. P.; Cui, W.; Dickherber, R.; Dumm, J.; Errando, M.; Falcone, A.; Federici, S.; Feng, Q.; Finley, J. P.; Finnegan, G.; Fortson, L.; Furniss, A.; Galante, N.; Gall, D.; Godambe, S.; Griffin, S.; Grube, J.; Gyuk, G.; Hanna, D.; Holder, J.; Huan, H.; Hughes, G.; Hui, C. M.; Imran, A.; Jameil, O.; Kaaret, P.; Karlsson, N.; Kertzman, M.; Kerr, J.; Khassen, Y.; Kieda, D.; Krawczynski, H.; Krennrich, F.; Lang, M. J.; Lee, K.; Madhavan, A. S.; Majumdar, P.; McArthur, S.; McCann, A.; Moriarty, P.; Mukherjee, R.; Nelson, T.; O'Faoláin de Bhróithe, A.; Ong, R. A.; Orr, M.; Otte, A. N.; Park, N.; Perkins, J. S.; Pichel, A.; Pohl, M.; Quinn, J.; Ragan, K.; Reynolds, P. T.; Roache, E.; Ruppel, J.; Saxon, D. B.; Schroedter, M.; Sembroski, G. H.; Şentürk, G. D.; Smith, A. W.; Staszak, D.; Stroh, M.; Telezhinsky, I.; Tešić, G.; Theiling, M.; Thibadeau, S.; Tsurusaki, K.; Varlotta, A.; Vassiliev, V. V.; Vivier, M.; Wakely, S. P.; Ward, J. E.; Weinstein, A.; Welsing, R.; Williams, D. A.; Zitzer, B.

    2012-08-01

    We present observations of the BL Lac object 1ES 0414+009 in the >200 GeV gamma-ray band by the VERITAS array of Cherenkov telescopes. 1ES 0414+009 was observed by VERITAS between 2008 January and 2011 February, resulting in 56.2 hr of good quality pointed observations. These observations resulted in a detection of 822 events from the source corresponding to a statistical significance of 6.4 standard deviations (6.4σ) above the background. The source flux, showing no evidence for variability, is measured as (5.2 ± 1.1stat ± 2.6sys) × 10-12 photons cm-2 s-1 above 200 GeV, equivalent to approximately 2% of the Crab Nebula flux above this energy. The differential photon spectrum from 230 GeV to 850 GeV is well fit by a power law with a photon index of Γ = 3.4 ± 0.5stat ± 0.3sys and a flux normalization of (1.6 ± 0.3stat ± 0.8sys) × 10-11 photons cm-2 s-1 at 300 GeV. We also present multiwavelength results taken in the optical (MDM), x-ray (Swift-XRT), and GeV (Fermi-LAT) bands and use these results to construct a broadband spectral energy distribution (SED). Modeling of this SED indicates that homogenous one-zone leptonic scenarios are not adequate to describe emission from the system, with a lepto-hadronic model providing a better fit to the data.

  7. Two-dimensional Langevin modeling of fission dynamics of the excited compound nuclei 188Pt, 227Pa and 251Es

    NASA Astrophysics Data System (ADS)

    Eslamizadeh, H.

    2016-02-01

    A stochastic approach based on one- and two-dimensional Langevin equations is applied to calculate the pre-scission neutron multiplicity, fission probability, anisotropy of fission fragment angular distribution, fission cross section and the evaporation cross section for the compound nuclei 188Pt, 227Pa and 251Es in an intermediate range of excitation energies. The chaos weighted wall and window friction formula are used in the Langevin equations. The elongation parameter, c, is used as the first dimension and projection of the total spin of the compound nucleus onto the symmetry axis, K, considered as the second dimension in Langevin dynamical calculations. A constant dissipation coefficient of K, γK = 0.077(MeV zs)-1/2, is used in two-dimensional calculations to reproduce the above mentioned experimental data. Comparison of the theoretical results of the pre-scission neutron multiplicity, fission probability, fission cross section and the evaporation cross section with the experimental data shows that the results of two-dimensional calculations are in better agreement with the experimental data. Furthermore, it is shown that the two-dimensional Langevin equations together with a dissipation coefficient of K, γK = 0.077(MeV zs)-1/2, can satisfactorily reproduce the anisotropy of fission fragment angular distribution for the heavy compound nucleus 251Es. However, a larger value of γK = 0.250(MeV zs)-1/2 is needed to reproduce the anisotropy of fission fragment angular distribution for the lighter compound nucleus 227Pa.

  8. Knowledge of diabetes and hypertension among members of teaching staff of higher learning institutions in Dar es Salaam, Tanzania.

    PubMed

    Mbuya, Faida E; Fredrick, Francis; Kundi, Beatus

    2014-04-01

    Diabetes and hypertension are among the most common non-communicable diseases (NCD) that contribute to a large number of adult morbidity and mortality. The objective of this study was to determine knowledge of diabetes and hypertension and the associated risk factors among members of teaching staff of Higher Learning Institutions in Dar es Salaam, Tanzania. A cross sectional community based study was conducted in 10 higher learning institutions including universities. A structured pretested questionnaire was utilized. A total of 139 participants were involved in this study. A total of 139 teaching members of staff from higher learning institutions participated in the study. The majority (74.8%; n=104) of the participants were males. Mean age of the participants was 40.7 ± 12.6. Over half (56.8%; 79/139) of the respondents correctly identified failure of body to use insulin as one of the causes of diabetes. Of the respondents, 43.2% (60/139) were able to identified heredity as cause of hypertension. Increasing age was correctly identified as one of risk factors for diabetes by 38.1% (53/139) and for hypertension by 36.7% (51/139) respondents. Thus knowledge of the causes, signs and symptoms, risk factors and complications was not as high as expected considering the respondents were among the highly educated and professional population. In conclusion, the majority of teaching staff in the higher learning Institutions in Dar es Salaam were aware of the diabetes and hypertension. However the knowledge of the causes, signs and symptoms, risk factors and complications was not as high as expected. It is important that this group of professionals is appropriately informed as regards to diabetes, hypertension and other non-communicable diseases as they may serve as key advocacy group to the community and policy makers in Tanzania.

  9. A TRANSIT TIMING ANALYSIS OF NINE RISE LIGHT CURVES OF THE EXOPLANET SYSTEM TrES-3

    SciTech Connect

    Gibson, N. P.; Pollacco, D.; Simpson, E. K.; Barros, S.; Joshi, Y. C.; Todd, I.; Keenan, F. P.; Skillen, I.; Benn, C.; Christian, D.; Hrudkova, M.; Steele, I. A.

    2009-08-01

    We present nine newly observed transits of TrES-3, taken as part of a transit timing program using the RISE instrument on the Liverpool Telescope. A Markov-Chain Monte Carlo analysis was used to determine the planet-star radius ratio and inclination of the system, which were found to be R{sub p} /R {sub *} = 0.1664{sup +0.0011} {sub -0.0018} and i = 81.73{sup +0.13} {sub -0.04}, respectively, consistent with previous results. The central transit times and uncertainties were also calculated, using a residual-permutation algorithm as an independent check on the errors. A re-analysis of eight previously published TrES-3 light curves was conducted to determine the transit times and uncertainties using consistent techniques. Whilst the transit times were not found to be in agreement with a linear ephemeris, giving {chi}{sup 2} = 35.07 for 15 degrees of freedom, we interpret this to be the result of systematics in the light curves rather than a real transit timing variation. This is because the light curves that show the largest deviation from a constant period either have relatively little out-of-transit coverage or have clear systematics. A new ephemeris was calculated using the transit times and was found to be T{sub c} (0) = 2454632.62610 {+-} 0.00006 HJD and P = 1.3061864 {+-} 0.0000005 days. The transit times were then used to place upper mass limits as a function of the period ratio of a potential perturbing planet, showing that our data are sufficiently sensitive to have probed sub-Earth mass planets in both interior and exterior 2:1 resonances, assuming that the additional planet is in an initially circular orbit.

  10. The long-term Swift observations of the high-energy peaked BL Lacertae source 1ES 1959+650

    NASA Astrophysics Data System (ADS)

    Kapanadze, B.; Romano, P.; Vercellone, S.; Kapanadze, S.; Mdzinarishvili, T.; Kharshiladze, G.

    2016-03-01

    We present the results based on the monitoring of the high-energy peaked BL Lacertae object 1ES 1959+650 by the Swift satellite during 2005-2014. Our timing study shows that the source was highly variable on longer (weeks-to-months) time-scales with the 0.3-10 keV fluxes ranging by a factor of 8. It sometimes showed a significant intra-day variability in the course of ˜1 ks, detected mainly in the epochs of higher brightness states. The flux variability exhibited an erratic character and no signatures of periodic variations are revealed. The X-ray spectra were mainly curved with broad ranges of photon index, curvature parameter, hardness ratio, synchrotron spectral energy distribution (SED) peak location which exhibited a significant variability with the flux at different time-scales. Our study of multi-wavelength cross-correlations shows that the one-zone synchrotron self-Compton scenario was not always valid for 1ES 1959+650. The X-ray flares were sometimes not accompanied with an increasing activity in the γ-ray or lower-energy parts of the spectrum and vice versa. Similar to the prominent `orphan' TeV event in 2002, significant flares in the high-energy and very high energy bands in 2009 May and 2012 May were not accompanied by those in the synchrotron part of the spectrum. Similar to other TeV-detected high-energy peaked BLLs, the stochastic acceleration of the electrons from the magnetic turbulence close to the shock front may be more important for our target compared to other scenarios since it showed mainly broader synchrotron SEDs during the X-ray flares expected when the stochastic mechanism is more efficient.

  11. Analysis of environment, safety, and health (ES{ampersand}H) management systems for Department of Energy (DOE) Defense Programs (DP) facilities

    SciTech Connect

    Neglia, A. V., LLNL

    1998-03-01

    The purpose of this paper is to provide a summary analysis and comparison of various environment, safety, and health (ES&H) management systems required of, or suggested for use by, the Departrnent of Energy Defense Programs` sites. The summary analysis is provided by means of a comparison matrix, a set of Vean diagrams that highlights the focus of the systems, and an `End Gate` filter diagram that integrates the three Vean diagrams. It is intended that this paper will act as a starting point for implementing a particular system or in establishing a comprehensive site-wide integrated ES&H management system. Obviously, the source documents for each system would need to be reviewed to assure proper implementation of a particular system. The matrix compares nine ES&H management systems against a list of elements generated by identifying the unique elements of all the systems. To simplify the matrix, the elements are listed by means of a brief title. An explanation of the matrix elements is provided in Attachment 2 entitled, `Description of System Elements.` The elements are categorized under the Total Quality Management (TQM) `Plan, Do, Check, Act` framework with the added category of `Policy`. (The TQM concept is explained in the `DOE Quality Management implementation Guidelines,` July 1997 (DOE/QM- 0008)). The matrix provides a series of columns and rows to compare the unique elements found in each of the management systems. A `V` is marked if the element is explicitly identified as part of the particular ES&H management system. An `X` is marked if the element is not found in the particular ES&H management system, or if it is considered to be inadequately addressed. A `?` is marked if incorporation of the element is not clear. Attachment I provides additional background information which explains the justification for the marks in the matrix cells. Through the Vean diagrams and the `End Gate` filter in Section 3, the paper attempts to pictorially display the focus of

  12. Overproduction of the Escherichia coli Chaperones GroEL-GroES in Rhodococcus ruber Improves the Activity and Stability of Cell Catalysts Harboring a Nitrile Hydratase.

    PubMed

    Tian, Yuxuan; Chen, Jie; Yu, Huimin; Shen, Zhongyao

    2016-02-01

    Three combinations of molecular chaperones from Escherichia coli (i.e., DnaK-DnaJ-GrpEGroEL- GroES, GroEL-GroES, and DnaK-DnaJ-GrpE) were overproduced in E. coli BL21, and their in vitro stabilizing effects on a nitrile hydratase (NHase) were assessed. The optimal gene combination, E. coli groEL-groES (ecgroEL-ES), was introduced into Rhodococcus ruber TH3. A novel engineered strain, R. ruber TH3G was constructed with the native NHase gene on its chromosome and the heterologous ecgroEL-ES genes in a shuttle plasmid. In R. ruber TH3G, NHase activity was enhanced 37.3% compared with the control, TH3. The in vivo stabilizing effect of ecGroEL-ES on the NHase was assessed using both acrylamide immersion and heat shock experiments. The inactivation behavior of the in vivo NHase after immersion in a solution of dynamically increased concentrations of acrylamide was particularly evident. When the acrylamide concentration was increased to 500 g/l (50%), the remaining NHase activity in TH3G was 38%, but in TH3, activity was reduced to 10%. Reactivation of the in vivo NHases after varying degrees of inactivation was further assessed. The activity of the reactivated NHase was more than 2-fold greater in TH3G than in TH3. The hydration synthesis of acrylamide catalyzed by the in vivo NHase was performed with continuous acrylonitrile feeding. The final concentration of acrylamide was 640 g/l when catalyzed by TH3G, compared with 490 g/l acrylamide by TH3. This study is the first to show that the chaperones ecGroEL-ES work well in Rhodococcus and simultaneously possess protein-folding assistance functions and the ability to stabilize and reactivate the native NHases. PMID:26562693

  13. Lignes directrices sur la prise en charge en soins primaires des céphalées chez l’adulte

    PubMed Central

    Becker, Werner J.; Findlay, Ted; Moga, Carmen; Scott, N. Ann; Harstall, Christa; Taenzer, Paul

    2015-01-01

    Résumé Objectif Accroître l’utilisation d’approches éclairées par des données probantes pour diagnostiquer, investiguer et traiter en soins primaires les céphalées des patients. Qualité des données Une recherche documentaire exhaustive a été effectuée pour trouver des lignes directrices et des révisions systématiques publiées entre janvier 2000 et mai 2011. La grille d’évaluation de la qualité des recommandations pour la pratique clinique AGREE a servi à l’analyse critique des lignes directrices. Les 6 guides de pratique clinique de la plus grande qualité ont servi de base dans le processus d’adaptation. Message principal Un groupe d’élaboration multidisciplinaire de professionnels des soins primaires ont formulé 91 recommandations précises à l’aide d’un processus consensuel. Les recommandations portent sur le diagnostic, l’investigation et la prise en charge de la migraine, de la céphalée de tension, de la céphalée due à une surmédication et de la céphalée vasculaire de Horton. Conclusion Un guide de pratique clinique adapté au contexte des soins de santé au Canada a été produit en suivant un processus d’adaptation de lignes directrices dans le but d’aider les professionnels de toutes les disciplines des soins primaires à dispenser des soins éclairés par des données probantes aux patients souffrant de céphalées.

  14. Une recension des écrits concernant la réalité psychoaffective des femmes ayant une vulvodynie : Difficultés rencontrées et stratégies développées

    PubMed Central

    Cantin-Drouin, Maude; Damant, Dominique; Turcotte, Daniel

    2008-01-01

    HISTORIQUE : Au cours des trois dernières décennies, une attention grandissante a été portée à l’étude du syndrome de vulvodynie, soit un inconfort vulvaire chronique inexpliqué, éprouvé en l’absence de pathologie précisée. En plus d’avoir des répercussions physiques, l’expérience de la vulvodynie comporte une dimension psychosociale. OBJECTIF : Cet article vise à faire une recension des écrits sur la réalité psychoaffective des femmes qui éprouvent une vulvodynie. MÉTHODE : Une recherche documentaire basée sur une exploration systématique de textes présentant des résultats de recherche et recensés dans les principales banques de données en sciences sociales, dont Dissertation Abstracts, Current Contents et PsycINFO, a été réalisée. RÉSULTATS : Cette recension des écrits révèle, malgré la présence de certains résultats divergents, que les femmes ayant une vulvodynie affrontent souvent des difficultés identitaires et psychologiques, qui sont influencées notamment par les normes sociales de sexualité et de féminité. Pour composer avec ces difficultés, les femmes développent différentes stratégies visant à diminuer le stress occasionné par la douleur et à améliorer leur bien-être psychologique. CONCLUSION : Les difficultés relationnelles et psychologiques vécues par les femmes ayant une vulvodynie ne découlent pas seulement de la douleur physique, mais aussi du sens qu’elles lui accordent, souvent influencé par les discours sociaux sur l’hétérosexualité et la féminité. Il importe donc d’intervenir auprès des femmes en tenant compte de l’influence du contexte social tout en poursuivant le développement des connaissances sur les aspects psychosociaux de cette expérience. PMID:18592063

  15. American Hero(es).

    ERIC Educational Resources Information Center

    Valore, Thomas G.

    2002-01-01

    Entering Re-ED (Reeducation of Emotional Disturbed Children) at age 15, Alex, spent three years working and learning together to reach the goals that Alex speaks to in his culminating story. Presents a transcript of a videotaped Petition to Graduate meeting held at the Positive Education Program as an example of the Re-ED philosophy, its…

  16. Elejalde syndrome (ES).

    PubMed

    Mohammadzadeh Shanehsaz, Siavash; Rezazadeh, Azadeh; Dandashli, Anwar

    2015-03-01

    Silvery hair and severe dysfunction of the central nervous system (Neuroectodermal melanolysosomal disease or Elejalde Syndrome) characterize this rare autosomal recessive syndrome. The main clinical features include silver-leaden hair, bronze skin after sun exposure, and neurologic involvement. Large granules of melanin unevenly distributed in the hair shaft are observed. Abnormal melanocytes and melanosomes and abnormal inclusion bodies in fibroblasts may be present. We report a 10-year-old girl with silver-leaden (silvery) hair, bronze skin color on sun-exposed areas, generalized hypopigmentation of covered body parts, and congenital seizures. The child was the elder of two children born of a consanguineous marriage. The younger sibling, a female neonate, had the same clinical presentation. PMID:25780981

  17. The 2 Es

    ERIC Educational Resources Information Center

    Kroog, Heidi; Hess, Kristin King; Ruiz-Primo, Maria Araceli

    2016-01-01

    What are the characteristics of formal formative assessments that are both effective in improving student learning and an efficient use of a teacher's time and efforts? That's the question that the authors explore in this article drawing on a five-year research study. First, formal formative assessment is defined as being planned in advance,…

  18. EsMlp, a muscle-LIM protein gene, is up-regulated during cold exposure in the freeze-avoiding larvae of Epiblema scudderiana.

    PubMed

    Bilgen, T; English, T E; McMullen, D C; Storey, K B

    2001-08-01

    Screening of a cDNA library identified transcripts that were up-regulated by cold (4 or -20 degrees C) exposure in larvae of the freeze-avoiding goldenrod gall moth, Epiblema scudderiana. One clone contained a full-length open reading frame encoding a protein of 94 amino acids. The gene product, with 79.1% of residues identical with the Drosophila LIM protein Mlp60A, was named EsMlp and contained a single LIM domain and consensus sequences characteristic of a LIM protein. Transcript levels rose approx twofold when larvae were shifted from 4 to -20 degrees C and approx threefold over the midwinter months compared with larvae sampled in October or April. EsMlp expression was high in larval head (possibly due to expression in pharyngeal muscles) and body wall but was not detected in fat body. Immunoblotting revealed a three- to fourfold increase in EsMlp protein in midwinter larvae (January-February) compared with November-collected animals and a further rise to eightfold higher than November values in larvae collected in April. Cold up-regulation of EsMlp and the pattern of EsMlp levels in the larvae suggest possible roles for the protein, such as in muscle maintenance over the winter or as a preparative function that could facilitate the rapid resumption of development and metamorphosis when environmental temperatures rise in the spring.

  19. Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors.

    PubMed

    Takayama, Naoya; Nishikii, Hidekazu; Usui, Joichi; Tsukui, Hiroko; Sawaguchi, Akira; Hiroyama, Takashi; Eto, Koji; Nakauchi, Hiromitsu

    2008-06-01

    Human embryonic stem cells (hESCs) could potentially represent an alternative source for blood transfusion therapies and a promising tool for studying the ontogeny of hematopoiesis. When we cultured hESCs on either C3H10T1/2 or OP-9 cells to facilitate hematopoiesis, we found that exogenous administration of vascular endothelial growth factor promoted the emergence of sac-like structures, which we named embryonic stem cell-derived sacs (ES-sacs). These ES-sacs consisted of multiple cysts demarcated by cellular monolayers that retained some of the properties of endothelial cells. The spherical cells inside ES-sacs expressed primarily CD34, along with VE-cadherin, CD31, CD41a, and CD45, and were able to form hematopoietic colonies in semisolid culture and to differentiate into mature megakaryocytes by day 24 in the presence of thrombopoietin. Apparently, ES-sacs provide a suitable environment for hematopoietic progenitors. Relatively large numbers of mature megakaryocytes could be induced from the hematopoietic progenitors within ES-sacs, which were then able to release platelets that displayed integrin alpha IIb beta 3 activation and spreading in response to ADP or thrombin. This novel protocol thus provides a means of generating platelets from hESCs, which could serve as the basis for efficient production of platelets for clinical transfusion and studies of thrombopoiesis.

  20. Urban morphological determinants of temperature regulating ecosystem services in African cities: the case of Dar es Salaam, Tanzania

    NASA Astrophysics Data System (ADS)

    Cavan, Gina; Lindley, Sarah; Kibassa, Deusdedit; Shemdoe, Riziki; Capuano, Paolo; De Paola, Francesco; Renner, Florian; Pauleit, Stephan

    2013-04-01

    Urban green structure provides important regulating ecosystem services, such as temperature and flood regulation, and thus, has the potential to increase the resilience of African cities to climate change. Green structures within urban areas are not only limited to discrete units associated with recreational parks, agricultural areas and open spaces: they also exist within zones which have other primary functions, such as church yards, along transport routes, and within residential areas. Differing characteristics of urban areas can be conceptualised and subsequently mapped through the idea of urban morphology types. Urban morphology types are classifications which combine facets of urban form and function. When mapped, UMT units provide biophysically relevant meso-scale geographical zones which can be used as the basis for understanding climate-related impacts and adaptations. For example, they support the assessment of urban temperature patterns and the temperature regulating services provided by urban green structures. There are some examples of the use of UMTs for assessing regulating ecosystem services in European cities but little similar knowledge is available in an African context. This paper outlines the concept of urban morphology types (UMTs) and how they were applied to African case study cities (Cavan et al., 2012). It then presents the methods used to understand temperature regulating ecosystem services across an example African case study city, including (i) a GIS-based assessment of urban green structures, and (ii) applying an energy balance model to estimate current and future surface temperatures under climate change projections. The assessment is carried out for Dar es Salaam, Tanzania. Existing evidence suggests increases in both mean and extreme temperatures in the city. Historical analysis of the number of hot days per year suggests a rise from a maximum of 47 days per year in the period 1961-87 to 72 days per year in 2003-2011 (Giugni et al