Science.gov

Sample records for reactant kit optimization

  1. Optimizing Medical Kits for Spaceflight

    NASA Technical Reports Server (NTRS)

    Keenan, A. B,; Foy, Millennia; Myers, G.

    2014-01-01

    The Integrated Medical Model (IMM) is a probabilistic model that estimates medical event occurrences and mission outcomes for different mission profiles. IMM simulation outcomes describing the impact of medical events on the mission may be used to optimize the allocation of resources in medical kits. Efficient allocation of medical resources, subject to certain mass and volume constraints, is crucial to ensuring the best outcomes of in-flight medical events. We implement a new approach to this medical kit optimization problem. METHODS We frame medical kit optimization as a modified knapsack problem and implement an algorithm utilizing a dynamic programming technique. Using this algorithm, optimized medical kits were generated for 3 different mission scenarios with the goal of minimizing the probability of evacuation and maximizing the Crew Health Index (CHI) for each mission subject to mass and volume constraints. Simulation outcomes using these kits were also compared to outcomes using kits optimized..RESULTS The optimized medical kits generated by the algorithm described here resulted in predicted mission outcomes more closely approached the unlimited-resource scenario for Crew Health Index (CHI) than the implementation in under all optimization priorities. Furthermore, the approach described here improves upon in reducing evacuation when the optimization priority is minimizing the probability of evacuation. CONCLUSIONS This algorithm provides an efficient, effective means to objectively allocate medical resources for spaceflight missions using the Integrated Medical Model.

  2. Optimizing Medical Kits for Space Flight

    NASA Technical Reports Server (NTRS)

    Minard, Charles G.; FreiredeCarvalho, Mary H.; Iyengar, M. Sriram

    2010-01-01

    The Integrated Medical Model (IMM) uses Monte Carlo methodologies to predict the occurrence of medical events, their mitigation, and the resources required during space flight. The model includes two modules that utilize output from a single model simulation to identify an optimized medical kit for a specified mission scenario. This poster describes two flexible optimization routines built into SAS 9.1. The first routine utilizes a systematic process of elimination to maximize (or minimize) outcomes subject to attribute constraints. The second routine uses a search and mutate approach to minimize medical kit attributes given a set of outcome constraints. There are currently 273 unique resources identified that are used to treat at least one of 83 medical conditions currently in the model.

  3. Mass and Volume Optimization of Space Flight Medical Kits

    NASA Technical Reports Server (NTRS)

    Keenan, A. B.; Foy, Millennia Hope; Myers, Jerry

    2014-01-01

    Resource allocation is a critical aspect of space mission planning. All resources, including medical resources, are subject to a number of mission constraints such a maximum mass and volume. However, unlike many resources, there is often limited understanding in how to optimize medical resources for a mission. The Integrated Medical Model (IMM) is a probabilistic model that estimates medical event occurrences and mission outcomes for different mission profiles. IMM simulates outcomes and describes the impact of medical events in terms of lost crew time, medical resource usage, and the potential for medically required evacuation. Previously published work describes an approach that uses the IMM to generate optimized medical kits that maximize benefit to the crew subject to mass and volume constraints. We improve upon the results obtained previously and extend our approach to minimize mass and volume while meeting some benefit threshold. METHODS We frame the medical kit optimization problem as a modified knapsack problem and implement an algorithm utilizing dynamic programming. Using this algorithm, optimized medical kits were generated for 3 mission scenarios with the goal of minimizing the medical kit mass and volume for a specified likelihood of evacuation or Crew Health Index (CHI) threshold. The algorithm was expanded to generate medical kits that maximize likelihood of evacuation or CHI subject to mass and volume constraints. RESULTS AND CONCLUSIONS In maximizing benefit to crew health subject to certain constraints, our algorithm generates medical kits that more closely resemble the unlimited-resource scenario than previous approaches which leverage medical risk information generated by the IMM. Our work here demonstrates that this algorithm provides an efficient and effective means to objectively allocate medical resources for spaceflight missions and provides an effective means of addressing tradeoffs in medical resource allocations and crew mission success

  4. Optimizing direct amplification of forensic commercial kits for STR determination.

    PubMed

    Caputo, M; Bobillo, M C; Sala, A; Corach, D

    2017-04-01

    Direct DNA amplification in forensic genotyping reduces analytical time when large sample sets are being analyzed. The amplification success depends mainly upon two factors: on one hand, the PCR chemistry and, on the other, the type of solid substrate where the samples are deposited. We developed a workflow strategy aiming to optimize times and cost when starting from blood samples spotted onto diverse absorbent substrates. A set of 770 blood samples spotted onto Blood cards, Whatman(®) 3 MM paper, FTA™ Classic cards, and Whatman(®) Grade 1 was analyzed by a unified working strategy including a low-cost pre-treatment, a PCR amplification volume scale-down, and the use of the 3500 Genetic Analyzer as the analytical platform. Samples were analyzed using three different commercial multiplex STR direct amplification kits. The efficiency of the strategy was evidenced by a higher percentage of high-quality profiles obtained (over 94%), a reduced number of re-injections (average 3.2%), and a reduced amplification failure rate (lower than 5%). Average peak height ratio among different commercial kits was 0.91, and the intra-locus balance showed values ranging from 0.92 to 0.94. A comparison with previously reported results was performed demonstrating the efficiency of the proposed modifications. The protocol described herein showed high performance, producing optimal quality profiles, and being both time and cost effective.

  5. DAKOTA Design Analysis Kit for Optimization and Terascale

    SciTech Connect

    Adams, Brian M.; Dalbey, Keith R.; Eldred, Michael S.; Gay, David M.; Swiler, Laura P.; Bohnhoff, William J.; Eddy, John P.; Haskell, Karen

    2010-02-24

    The DAKOTA (Design Analysis Kit for Optimization and Terascale Applications) toolkit provides a flexible and extensible interface between simulation codes (computational models) and iterative analysis methods. By employing object-oriented design to implement abstractions of the key components required for iterative systems analyses, the DAKOTA toolkit provides a flexible and extensible problem-solving environment for design and analysis of computational models on high performance computers.A user provides a set of DAKOTA commands in an input file and launches DAKOTA. DAKOTA invokes instances of the computational models, collects their results, and performs systems analyses. DAKOTA contains algorithms for optimization with gradient and nongradient-based methods; uncertainty quantification with sampling, reliability, polynomial chaos, stochastic collocation, and epistemic methods; parameter estimation with nonlinear least squares methods; and sensitivity/variance analysis with design of experiments and parameter study methods. These capabilities may be used on their own or as components within advanced strategies such as hybrid optimization, surrogate-based optimization, mixed integer nonlinear programming, or optimization under uncertainty. Services for parallel computing, simulation interfacing, approximation modeling, fault tolerance, restart, and graphics are also included.

  6. DAKOTA Design Analysis Kit for Optimization and Terascale

    SciTech Connect

    Adams, Brian M.; Dalbey, Keith R.; Eldred, Michael S.; Gay, David M.; Swiler, Laura P.; Bohnhoff, William J.; Eddy, John P.; Haskell, Karen

    2010-02-24

    The DAKOTA (Design Analysis Kit for Optimization and Terascale Applications) toolkit provides a flexible and extensible interface between simulation codes (computational models) and iterative analysis methods. By employing object-oriented design to implement abstractions of the key components required for iterative systems analyses, the DAKOTA toolkit provides a flexible and extensible problem-solving environment for design and analysis of computational models on high performance computers.A user provides a set of DAKOTA commands in an input file and launches DAKOTA. DAKOTA invokes instances of the computational models, collects their results, and performs systems analyses. DAKOTA contains algorithms for optimization with gradient and nongradient-based methods; uncertainty quantification with sampling, reliability, polynomial chaos, stochastic collocation, and epistemic methods; parameter estimation with nonlinear least squares methods; and sensitivity/variance analysis with design of experiments and parameter study methods. These capabilities may be used on their own or as components within advanced strategies such as hybrid optimization, surrogate-based optimization, mixed integer nonlinear programming, or optimization under uncertainty. Services for parallel computing, simulation interfacing, approximation modeling, fault tolerance, restart, and graphics are also included.

  7. Development and Optimization of a Homemade ELISA Kit for Detection of Antibodies Against Haemophilus influenzae Type b

    PubMed Central

    Mousavi, Seyed Fazlolah; Fatemi, Sara; Siadat, Seyed Davar; Zahraei, Seyed Mohsen; Nikanpour, Elnaz; Malekan, Mohamad Ali; Khabiri, Ali Reza; Janani, Ali Reza

    2016-01-01

    Background: Haemophilus influenzae type b (Hib) infection has high morbidity and mortality rate, especially in children under 5 years of age. Enzyme-linked immunosorbent assay (ELISA) technique is the most used method to detect antibodies against H. influenzae. Available commercial ELISA kits are expensive and not always readily available, particularly for epidemiological studies. Objectives: This study was performed to develop and optimize a homemade ELISA kit for the detection of Hib anti-polyribosylribitol phosphate (PRP) antibodies in children. Materials and Methods: To develop and optimize an indirect ELISA method, pure PRP was prepared. The PRP was coupled to bovine serum albumin, using sodium periodate. Then optimal conditions for ELISA, including coating antigen concentration and peroxidase labeled conjugate concentrations, incubation temperature and incubation time, were determined. To confirm the efficacy of optimized kit, 83 serum samples from non-vaccinated children, aged less than 6 years were collected and analyzed, using homemade and commercial ELISA. Results: The optimal conditions were considered to perform ELISA. Comparison between results obtained from optimized ELISA kit and commercial ELISA kit showed a good agreement. Conclusions: Taking into account these data, we elaborated a homemade ELISA kit that is an efficacious and cost-effective substitute for commercial kit, in disease control and diagnosis. PMID:27540453

  8. Optimization Routine for Generating Medical Kits for Spaceflight Using the Integrated Medical Model

    NASA Technical Reports Server (NTRS)

    Graham, Kimberli; Myers, Jerry; Goodenow, Deb

    2017-01-01

    The Integrated Medical Model (IMM) is a MATLAB model that provides probabilistic assessment of the medical risk associated with human spaceflight missions.Different simulations or profiles can be run in which input conditions regarding both mission characteristics and crew characteristics may vary. For each simulation, the IMM records the total medical events that occur and “treats” each event with resources drawn from import scripts. IMM outputs include Total Medical Events (TME), Crew Health Index (CHI), probability of Evacuation (pEVAC), and probability of Loss of Crew Life (pLOCL).The Crew Health Index is determined by the amount of quality time lost (QTL). Previously, an optimization code was implemented in order to efficiently generate medical kits. The kits were optimized to have the greatest benefit possible, given amass and/or volume constraint. A 6-crew, 14-day lunar mission was chosen for the simulation and run through the IMM for 100,000 trials. A built-in MATLAB solver, mixed-integer linear programming, was used for the optimization routine. Kits were generated in 10% increments ranging from 10%-100% of the benefit constraints. Conditions wheremass alone was minimized, volume alone was minimized, and where mass and volume were minimizedjointly were tested.

  9. Evaluation of two high-abundance protein depletion kits and optimization of downstream isoelectric focusing.

    PubMed

    Qiu, Fanghua; Hou, Tieying; Huang, Dehong; Xue, Zhifeng; Liang, Dongyan; Li, Qiuming; Lin, Weimiao

    2015-11-01

    Disease biomarkers for diagnostic and prognostic purposes are most likely within an extremely low concentration range and are thus masked by the presence of high‑abundance proteins. Therefore, removing high‑abundance proteins is the main challenge for identifying disease biomarkers. In addition, the solution obtained from high‑abundance protein depletion kits contains a rich array of compounds, which interfere with isoelectric focusing (IEF). In the present study, the effect of two commercial kits was evaluated and the downstream IEF protocol was optimized. High‑resolution results could be obtained according to the following conditions: The ProteoPrep Blue Albumin and IgG Depletion kit depleted albumin and IgG; immobilized pH gradient strips (typically 18 cm) were rehydrated with sample buffer containing 250 µg serum proteins at 30 v for 6 h, 60 v for 6 h, 200 v for 2 h, 500 v for 2 h, 1,000 v for 2 h, 5,000 v for 2 h, 10,000 v for 2 h and then focusing at 10,000 v up to 110 k vhs. In addition, the protein spots identified by matrix‑assisted laser desorption ionization time‑of‑flight mass spectrometry demonstrated that all proteins had a low abundance. The present study not only provides a definite and effective method for removing high‑abundance proteins, but also provides a proper protocol (protocol C) for downstream IEF. The present study includes a comprehensive investigation of serum proteomics, which paves the way for serum protein research.

  10. Transport Studies Enabling Efficiency Optimization of Cost-Competitive Fuel Cell Stacks (aka AURORA: Areal Use and Reactant Optimization at Rated Amperage)

    SciTech Connect

    Conti, Amedeo; Dross, Robert

    2013-12-06

    Hydrogen fuel cells are recognized as one of the most viable solutions for mobility in the 21st century; however, there are technical challenges that must be addressed before the technology can become available for mass production. One of the most demanding aspects is the costs of present-day fuel cells which are prohibitively high for the majority of envisioned markets. The fuel cell community recognizes two major drivers to an effective cost reduction: (1) decreasing the noble metals content, and (2) increasing the power density in order to reduce the number of cells needed to achieve a specified power level. To date, the majority of development work aimed at increasing the value metric (i.e. W/mg-Pt) has focused on the reduction of precious metal loadings, and this important work continues. Efforts to increase power density have been limited by two main factors: (1) performance limitations associated with mass transport barriers, and (2) the historical prioritization of efficiency over cost. This program is driven by commercialization imperatives, and challenges both of these factors. The premise of this Program, supported by proprietary cost modeling by Nuvera, is that DOE 2015 cost targets can be met by simultaneously exceeding DOE 2015 targets for Platinum loadings (using materials with less than 0.2 mg-Pt/cm2) and MEA power density (operating at higher than 1.0 Watt/cm2). The approach of this program is to combine Nuvera’s stack technology, which has demonstrated the ability to operate stably at high current densities (> 1.5 A/cm2), with low Platinum loading MEAs developed by Johnson Matthey in order to maximize Pt specific power density and reduce stack cost. A predictive performance model developed by PSU/UTK is central to the program allowing the team to study the physics and optimize materials/conditions specific to low Pt loading electrodes and ultra-high current density and operation.

  11. Optimization of the RNeasy Mini Kit to obtain high-quality total RNA from sessile cells of Staphylococcus aureus.

    PubMed

    Beltrame, C O; Côrtes, M F; Bandeira, P T; Figueiredo, A M S

    2015-12-01

    Biofilm formed by Staphylococcus aureus is considered an important virulence trait in the pathogenesis of infections associated with implantable medical devices. Gene expression analyses are important strategies for determining the mechanisms involved in production and regulation of biofilm. Obtaining intact RNA preparations is the first and most critical step for these studies. In this article, we describe an optimized protocol for obtaining total RNA from sessile cells of S. aureus using the RNeasy Mini Kit. This method essentially consists of a few steps, as follows: 1) addition of acetone-ethanol to sessile cells, 2) lysis with lysostaphin at 37°C/10 min, 3) vigorous mixing, 4) three cycles of freezing and thawing, and 5) purification of the lysate in the RNeasy column. This simple pre-kit procedure yields high-quality total RNA from planktonic and sessile cells of S. aureus.

  12. Optimization of pH values to formulate the bireagent kit for serum uric acid assay.

    PubMed

    Huang, Ya; Chen, Yuanxiang; Yang, Xiaolan; Zhao, Hua; Hu, Xiaolei; Pu, Jun; Liao, Juan; Long, Gaobo; Liao, Fei

    2015-01-01

    A new formulation of the bireagent kit for serum uric acid assay was developed based on the effects of pH on enzyme stability. At 4 °C, half-lives of uricases from Bacillus fastidious and Arthrobacter globiforms were longer than 15 months at pH 9.2, but became shorter at pH below 8.0; half-lives of ascorbate oxidase and peroxidase were comparable at pH 6.5 and 7.0, but became much shorter at pH higher than 7.4. In the new formulation of the bireagent kit, Reagent A contained peroxidase, 4-aminoantipyrine, and ascorbate oxidase in 50 mM phosphate buffer at pH 6.5; Reagent B contained B. fastidious or A. globiforms uricase in 50 mM sodium borate buffer at pH 9.2; Reagents A and B were mixed at 4:1 to produce a final pH from 7.2 to 7.6 for developing a stable color. The new bireagent kit consumed smaller quantities of three enzymes for the same shelf life. With the new bireagent kit, there were linear responses of absorbance at 546 nm to uric acid up to 34 mM in reaction mixtures and a good correlation of uric acid levels in clinical sera with those by a commercial kit, but stronger resistance to ascorbate. Therefore, the new formulation was advantageous. © 2014 International Union of Biochemistry and Molecular Biology, Inc.

  13. An ontology to enable optimized task partitioning in human-robot collaboration for warehouse kitting operations

    NASA Astrophysics Data System (ADS)

    Banerjee, Ashis Gopal; Barnes, Andrew; Kaipa, Krishnanand N.; Liu, Jiashun; Shriyam, Shaurya; Shah, Nadir; Gupta, Satyandra K.

    2015-06-01

    Collaborative teams of human operators and mobile ground robots are becoming popular in manufacturing plants to assist humans with a lot of the repetitive tasks such as the packing of related objects into different units, an operation known as kitting. In this paper, we present an ontology to provide a unified representation of all kitting-related tasks, which are decomposed into atomic actions that are either computational involving sensing, perception, planning, and control, or physical involving actuation and manipulation. The ontology is then used in a stochastic integer linear program for optimum partitioning of the atomic tasks between the robots and humans. Preliminary experiments on a single robot, single human case yield promising results where the kitting operations are completed with lower durations and manipulation failure rates using human-robot partnership versus just the human or only the robot. This success is achieved by the robot seeking human assistance for visual perception tasks while performing the other tasks primarily on its own.

  14. Survival Kit - Food Kit

    NASA Image and Video Library

    1962-10-01

    S62-08742 (1962) --- Food kit used by Mercury astronauts. Some is dehydrated and needs water, other packets are ready to eat. Size is measured relative to a ruler. Included are packets of mushroom soup, orange-grapefruit juice, cocoa beverage, pineapple juice, chicken with gravy, pears, strawberries, beef and vegetables and other assorted food containers. Photo credit: NASA

  15. Survival Kit - Food Kit

    NASA Image and Video Library

    1962-10-01

    S62-08743 (1962) --- Food kit used by Mercury astronauts. Some is dehydrated and needs water, other packets are ready to eat. Size is measured relative to a ruler. Included are packets of mushroom soup, orange-grapefruit juice, cocoa beverage, pineapple juice, chicken with gravy, pears, strawberries, beef and vegetables and other assorted food containers. Photo credit: NASA

  16. Network Kits.

    ERIC Educational Resources Information Center

    Falk, Howard

    1999-01-01

    Describes interconnection methods, speed, and comparative equipment costs of networking starter kits. These kits supply network-connection devices that plug into or connect to each computer that is part of a network; they may also provide interconnection cables and installation software needed to set up a network. Reviews 20 kits that use a…

  17. Optimizing reserve expansion for disjunct populations of San Joaquin kit fox

    Treesearch

    Robert G. Haight; Brian Cypher; Patrick A. Kelly; Scott Phillips; Katherine Ralls; Hugh P. Possingham

    2004-01-01

    Expanding habitat protection is a common strategy for species conservation. We present a model to optimize the expansion of reserves for disjunct populations of an endangered species. The objective is to maximize the expected number of surviving populations subject to budget and habitat constraints. The model accounts for benefits of reserve expansion in terms of...

  18. Monitoring of MOCVD reactants by UV absorption

    SciTech Connect

    Baucom, K.C.; Killeen, K.P.; Moffat, H.K.

    1995-07-01

    In this paper, we describe how UV absorption measurements can be used to measure the flow rates of metal organic chemical vapor deposition (MOCVD) reactants. This method utilizes the calculation of UV extinction coefficients by measuring the total pressure and absorbance in the neat reactant system. The development of this quantitative reactant flow rate monitor allows for the direct measurement of the efficiency of a reactant bubbler. We demonstrate bubbler efficiency results for TMGa, and then explain some discrepancies found in the TMAl system due to the monomer to dimer equilibrium. Also, the UV absorption spectra of metal organic and hydride MOCVD reactants over the wavelength range 185 to 400 nm are reported.

  19. Design Analysis Kit for Optimization and Terascale Applications 6.0

    SciTech Connect

    2015-10-19

    Sandia's Dakota software (available at http://dakota.sandia.gov) supports science and engineering transformation through advanced exploration of simulations. Specifically it manages and analyzes ensembles of simulations to provide broader and deeper perspective for analysts and decision makers. This enables them to: (1) enhance understanding of risk, (2) improve products, and (3) assess simulation credibility. In its simplest mode, Dakota can automate typical parameter variation studies through a generic interface to a computational model. However, Dakota also delivers advanced parametric analysis techniques enabling design exploration, optimization, model calibration, risk analysis, and quantification of margins and uncertainty with such models. It directly supports verification and validation activities. The algorithms implemented in Dakota aim to address challenges in performing these analyses with complex science and engineering models from desktop to high performance computers.

  20. Design Analysis Kit for Optimization and Terascale Applications 6.0

    SciTech Connect

    2015-10-19

    Sandia's Dakota software (available at http://dakota.sandia.gov) supports science and engineering transformation through advanced exploration of simulations. Specifically it manages and analyzes ensembles of simulations to provide broader and deeper perspective for analysts and decision makers. This enables them to: (1) enhance understanding of risk, (2) improve products, and (3) assess simulation credibility. In its simplest mode, Dakota can automate typical parameter variation studies through a generic interface to a computational model. However, Dakota also delivers advanced parametric analysis techniques enabling design exploration, optimization, model calibration, risk analysis, and quantification of margins and uncertainty with such models. It directly supports verification and validation activities. The algorithms implemented in Dakota aim to address challenges in performing these analyses with complex science and engineering models from desktop to high performance computers.

  1. Optimization of 384-well Luminex Immunoassays on the FlexMAP 3D System for Routine Analyte Quantitation from Commercial Kits

    PubMed Central

    Pergande, Melissa; Pithadia, Ravi; Borgia, Jeffrey A.; Fhied, Cristina

    2014-01-01

    INTRODUCTION: The Luminex immunobead platform offers the benefits of ELISA-based assays, but also permits higher throughput, increased flexibility, reduced sample volume, and lower cost when evaluation of multiple analytes is necessary. The FlexMap 3D system is a compact, high bead throughput instrument which can read up to 500 different bead regions from a single sample in a 96 or 384-well plate format. Commercially-available kits for the measurement of protein analytes are currently only available in the 96-well plate format. The objective of this study was to adapt and validate the commonly used 96-well plate protocols to the 384-well plate format with equivalent or improved test performance characteristics. METHODS: A laboratory-developed 96-well plate assay protocol for Vimentin autoantibody quantitation was translated and optimized for a 384-well plate format. Assay sensitivity was evaluated by comparing the results from two commercially available kits (Human IGF Binding Proteins 1-7 and Human IGF I/II), first employing the traditional 96-well plate format and subsequently using the newly optimized 384-well plate format. RESULTS: Conversion from the 96 to 384-well plate format for our Vimentin autoantibody assay revealed comparable sensitivity, while consuming only about a quarter of the reagents and specimen. Results from the IGF-I assay revealed approximately equivalent sensitivity (±2.4%), whereas IGF-II showed a significant increase in sensitivity (17.7%), both when the 96 and 384-well plate formats were ran concomitantly. No significant alterations in assay range, recovery or precision were otherwise noted. CONCLUSION: In this study, we have demonstrated that the conversion of a 96 to 384-well plate format was developed and validated successfully. When employing our optimized 384-well plate format, we found that nearly 4 times as many samples can be ran additionally with one commercially available kit, saving in not only cost but also sample size.

  2. Solid Reactants for Fluorine Gas Generators

    DTIC Science & Technology

    1976-03-15

    VOBrF 4 . The two interhalogen derivatives, KDrF 6 and KCIF 4 , were carried through mall-scale grain testing (10 to 20 g). Test data shoved the KBrF 6...Ig 3N2 and KCIF4 /AIN reactant systems to be most amenable to ignition and combustion in the scale tested . Studies of these two reactant systemd mere...systems mere difficult to achieve on the scale tested . The most promising candidate based on the grains tested consisted of a LiMnF 5/M formulation. An

  3. Preparation of clinical-scale (177) Lu-Rituximab: Optimization of protocols for conjugation, radiolabeling and freeze-dried kit formulation.

    PubMed

    Guleria, Mohini; Das, Tapas; Kumar, Chandan; Amirdhanayagam, Jeyachitra; Sarma, Haladhar D; Banerjee, Sharmila

    2017-02-08

    Rituximab is a monoclonal chimeric antibody which has been approved by US FDA for immunotherapy of Non-Hodgkins' lymphoma (NHL). Bexxar and Zevalin are the two other approved radiolabeled antibodies for radioimmunotherapy of NHL; however the fact that they are of murine origin reduces their treatment efficacy. To circumvent this, efforts have been made to radiolabel Rituximab with various therapeutic radioisotopes. In the present study, an effort has been made to optimize the conjugation (BFCA and antibody) and radiolabeling procedures for the preparation of clinical-scale (177) Lu-labeled Rituximab. An attempt was also made to prepare the freeze-dried Rituximab kit for the easy and convenient clinical translation of the agent. Clinical-scale (177) Lu-Rituximab (40 mCi, 1.48 GBq) was prepared with >95% radiochemical purity using the kit. Biological evaluation of (177) Lu-Rituximab was carried out by in-vitro cell binding studies in Raji cell lines, which showed satisfactory binding at 4 and 37 °C. Pharmacokinetic behaviour of the agent, evaluated by biodistribution studies in normal Swiss mice, revealed high blood and liver uptake at the initial time points; although it exhibited slow and gradual clearance with time. The study indicates that clinical-scale (177) Lu-Rituximab could be conveniently formulated using the methodology described in the present article.

  4. Compact mixed-reactant fuel cells

    NASA Astrophysics Data System (ADS)

    Priestnall, Michael A.; Kotzeva, Vega P.; Fish, Deborah J.; Nilsson, Eva M.

    The compact mixed-reactant (CMR) fuel cell is an important new "platform" approach to the design and operation of all types of fuel cell stacks. Amongst several other advantages, CMR has the potential to reduce polymer electrolyte membrane (PEM) stack component costs by around a third and to raise volumetric power densities by an order of magnitude. Mixed-reactant fuel cells, in which the fuel and oxidant within a cell are allowed to mix, rely upon the selectivity of anode and cathode electrocatalysts to separate the electrochemical oxidation of fuel and reduction of oxidant. A comprehensive review of the 50-year history of mixed-reactant literature has demonstrated that such systems can perform as well as and, in some circumstances, much better than conventional fuel cells. The significant innovation that Generics has introduced to this field is to combine the concept of mixed-reactant fuel cells with that of a fully porous membrane electrode assembly (MEA) structure. Passing a fuel-oxidant mixture through a stack of porous cells allows the conventional bipolar flow-field plates required in many fuel cell designs to be eliminated. In a conventional PEM stack, for example, the bipolar carbon flow-field plates may block up to half of the active cell area and account for up to 90% of the volume of the stack and of the order of one-third of the materials costs. In addition to all the advantages of mixed-reactant systems, the "flow-through" mode, embodied in Generics' CMR approach, significantly enhances mass-transport of reactants to the electrodes and can reduce reactant pressure drops across the stack. Redesigning fuel cells to operate in a CMR mode with selective electrodes offers the attractive prospect of much reduced stack costs and significantly higher stack power densities for all types of fuel cell. Initial modeling and proof of principle experiments using an alkaline system have confirmed the validity of the CMR approach and the potential for substantial

  5. Protein crystallography prescreen kit

    DOEpatents

    Segelke, Brent W.; Krupka, Heike I.; Rupp, Bernhard

    2007-10-02

    A kit for prescreening protein concentration for crystallization includes a multiplicity of vials, a multiplicity of pre-selected reagents, and a multiplicity of sample plates. The reagents and a corresponding multiplicity of samples of the protein in solutions of varying concentrations are placed on sample plates. The sample plates containing the reagents and samples are incubated. After incubation the sample plates are examined to determine which of the sample concentrations are too low and which the sample concentrations are too high. The sample concentrations that are optimal for protein crystallization are selected and used.

  6. Protein crystallography prescreen kit

    DOEpatents

    Segelke, Brent W.; Krupka, Heike I.; Rupp, Bernhard

    2005-07-12

    A kit for prescreening protein concentration for crystallization includes a multiplicity of vials, a multiplicity of pre-selected reagents, and a multiplicity of sample plates. The reagents and a corresponding multiplicity of samples of the protein in solutions of varying concentrations are placed on sample plates. The sample plates containing the reagents and samples are incubated. After incubation the sample plates are examined to determine which of the sample concentrations are too low and which the sample concentrations are too high. The sample concentrations that are optimal for protein crystallization are selected and used.

  7. An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments

    PubMed Central

    Heyer, Erin E.; Ozadam, Hakan; Ricci, Emiliano P.; Cenik, Can; Moore, Melissa J.

    2015-01-01

    Deep sequencing of strand-specific cDNA libraries is now a ubiquitous tool for identifying and quantifying RNAs in diverse sample types. The accuracy of conclusions drawn from these analyses depends on precise and quantitative conversion of the RNA sample into a DNA library suitable for sequencing. Here, we describe an optimized method of preparing strand-specific RNA deep sequencing libraries from small RNAs and variably sized RNA fragments obtained from ribonucleoprotein particle footprinting experiments or fragmentation of long RNAs. Our approach works across a wide range of input amounts (400 pg to 200 ng), is easy to follow and produces a library in 2–3 days at relatively low reagent cost, all while giving the user complete control over every step. Because all enzymatic reactions were optimized and driven to apparent completion, sequence diversity and species abundance in the input sample are well preserved. PMID:25505164

  8. Exhaust gas treatment by a reactant

    SciTech Connect

    Skala, S.F.

    1982-07-13

    In a system and process which forms reaction products particulates in an exhaust gas, a substantial portion of the reaction products are separated in a concentrated form by a first stage for delivery to a collector and reaction products remaining in the exhaust gas are separated by a final stage which incorporates the reaction products in one reactant. The reactant incorporating the reaction products subsequently reacts and repeatedly passes through the stages of separation thereby collecting substantially all of the reaction products in concentrated form by the first stage and providing a clean exhaust gas after the final stage without adding substantially to system weight or complexity. In the preferred embodiment of a vehicle system having an internal combustion engine based on a reaction of NaK with water in the presence of air, NaK hydroxide particulate reaction products are separated from a nitrogen exhaust gas in a first stage impingement separator, are accumulated in a final stage filter, and are dissolved by the water which subsequently reacts with nak.

  9. Kits in Motion

    ERIC Educational Resources Information Center

    Gee, Maureen

    1975-01-01

    Discusses three kits developed by museums in British Columbia for use in rural classrooms. The science kit on marine biology consists of modules which included specimens, books, audiovisual materials and student activities. (BR)

  10. Kits in Motion

    ERIC Educational Resources Information Center

    Gee, Maureen

    1975-01-01

    Discusses three kits developed by museums in British Columbia for use in rural classrooms. The science kit on marine biology consists of modules which included specimens, books, audiovisual materials and student activities. (BR)

  11. GridKit

    SciTech Connect

    Peles, Slaven

    2016-11-06

    GridKit is a software development kit for interfacing power systems and power grid application software with high performance computing (HPC) libraries developed at National Labs and academia. It is also intended as interoperability layer between different numerical libraries. GridKit is not a standalone application, but comes with a suite of test examples illustrating possible usage.

  12. Levitation Kits Demonstrate Superconductivity.

    ERIC Educational Resources Information Center

    Worthy, Ward

    1987-01-01

    Describes the "Project 1-2-3" levitation kit used to demonstrate superconductivity. Summarizes the materials included in the kit. Discusses the effect demonstrated and gives details on how to obtain kits. Gives an overview of the documentation that is included. (CW)

  13. Levitation Kits Demonstrate Superconductivity.

    ERIC Educational Resources Information Center

    Worthy, Ward

    1987-01-01

    Describes the "Project 1-2-3" levitation kit used to demonstrate superconductivity. Summarizes the materials included in the kit. Discusses the effect demonstrated and gives details on how to obtain kits. Gives an overview of the documentation that is included. (CW)

  14. Acceleration of disproportionation of aromatic alcohols through self-emulsification of reactants in water.

    PubMed

    Zhang, Binbin; Song, Jinliang; Liu, Huizhen; Han, Buxing; Jiang, Tao; Fan, Honglei; Zhang, Zhaofu; Wu, Tianbin

    2012-12-01

    Exploration of new and effective routes to conduct organic reactions in water using the special properties of water/organics is of great importance. In this work, we performed the disproportionation of various aromatic alcohols in water and in different organic solvents. It was demonstrated that the disproportionation reactions of the alcohols were accelerated more effectively in water than organic-solvent-based or solvent-free reactions. A series of control experiments were conducted to study the mechanism of the accelerated reaction rate in water. It was shown that the reactants could emulsify the reactant/water systems at the reaction conditions owing to their amphiphilic nature. The regularly orientated reactant molecules at the water/reactant droplet interface improved the contact probability of the reactive groups and the Pd nanocatalysts, which is one of the main reasons for the enhanced reaction rate in water. Controlling the self-emulsification of amphiphilic reactant/water systems has great application potential for optimizing the rate and/or selectivity of many organic reactions.

  15. [Leptin--a new acute phase reactant].

    PubMed

    Maruna, P; Gürlich, R; Frasko, R

    2001-07-01

    Leptin, 16 kDa protein and the product of the Ob gene was discovered six years ago and characterized as a fat cell hormone which maintains via specific receptors in the hypothalamus by a feedback mechanism the energy balance and body weight. Research conducted during the last three years detected further properties of this protein which include it, in addition to its basic role in mammalian metabolism, also among acute phase reactants. Leptin is as to its structure, pattern of the receptor and the postreceptor transduction mechanism close to the group of cytokines of interleukin 6, in particular the granulocytic colony stimulating factor. During infectious and non-infectious inflammatory reactions leptin synthesis is stimulated by a combination of inflammation promoting cytokines. The mechanism of induction is not known so far but the majority of investigations proves an indirect stimulating effect of the tumour necrosis factor and interleukin 1, depending on non-specified cytokines of the second stage. Investigations of cell lines and animal experiments provided evidence of some local and systemic effects of leptin which may play a physiological part in the acute phase reaction. Leptin mediates at least partly the anorectic effect of the tumour necrosis factor and interleukin 1 during inflammation. Synergically with bacterial antigens it activates macrophages, enhances their phagocytic capacity and stimulates secretion of pro- and anti-inflammatory factors from macrophages. Leptin stimulates neovascularization, it has a stimulating effect on precursors of white blood cells and potentiates the effect of erythropoietin on red blood cells. It is obviously an essential factor for T-lymphocyte proliferation and development. The authors discuss also the role of leptin in the modulation of the hypothalamo-pituito-adrenal stress axis. Leptin is a factor of the inflammatory mediator network, probably essential for an adequate course of the inflammatory defence reaction.

  16. Selectivity and mixed reactant fuel cells

    NASA Astrophysics Data System (ADS)

    Riess, Ilan

    2015-01-01

    Mixed reactant fuel cells (MR-FCs), are aimed at using a uniform mixture of fuel and oxygen applied to both the anode and the cathode. This allows redesign of fuel cells with a significantly simpler construction, having potentially a higher power density, better fuel utilization and be less expensive. The challenge in realizing MR-FCs is finding selective electrodes that can enhance oxygen reduction at the cathode, fuel oxidation at the anode while inhibiting the chemical reaction between the fuel and oxygen in the gas mixture. This task is in particular challenging in solid oxide fuel cells (SOFCs), as they operate at elevated temperatures, where many reactions are easily activated and selectivity is difficult to achieve. As a result no true MR-FC of the SOFC type were reported while some were found for low temperature fuel cells (FCs). The so-called single-chamber-SOFC are not true MR-FCs as they do not contain two selective electrodes, as required. We shall discuss potential ways to search for and develop selective anodes and cathodes for SOFC type MR-FCs. We first consider material properties which should contribute to that goal. This refers to electronic properties of the bulk, band banding under adsorbed specie, point defects in the bulk and on the surface. We then proceed to show how cell design, in particular electrode structure, can contribute to selectivity. Finally operation conditions are considered and it is shown that they also can contribute to selectivity. The operation condition considered are gas mixture composition, gas mixture residence time in the hot zone, hence gas flow rate, current density and temperature. The topics discussed hold for all FC types but are crucial for the SOFC type because of the difficulty to achieve selectivity at elevated temperatures. It is suggested that a concerted effort taking advantage of all those options should allow development of a true SOFC type MR-FC.

  17. A miniaturized kit for ozone biomonitoring

    SciTech Connect

    Lorenzini, G.

    1994-07-01

    A new miniaturized kit based on very young supersensitive tobacco Bel-W3 plantlets, which can be easily used to detect phytotoxic levels of ozone in ambient air in large scale surveys, is described. It has been developed in laboratory as well as field studies. The optimal sampling time is 5-7 d. The advantages of the kit are its user-friendliness, low cost, and reliability. The kit may be integrated by a passive sampling tube set and may be also proposed for educational programs. 6 refs., 2 figs.

  18. Creation of learning kits

    NASA Technical Reports Server (NTRS)

    Stow, D. A.; Estes, J. E.; Mertz, F. C.

    1981-01-01

    A learning kit is an essential part of any remote sensing workshop, course, or in-house training program to provide the "hands-on" experience of working with remotely sensed imagery. This is the objective of laboratory and field exercises as well as the reason behind the production of imagery/map kits. The way in which these learning kits (containing conventional remotely sensed and collateral data products) are put together is described and some concerns that influence the creation of learning kits are discussed. These include budgetary constraints, number of imagery types, and number of collateral data types.

  19. Creation of learning kits

    NASA Technical Reports Server (NTRS)

    Stow, D. A.; Estes, J. E.; Mertz, F. C.

    1981-01-01

    A learning kit is an essential part of any remote sensing workshop, course, or in-house training program to provide the "hands-on" experience of working with remotely sensed imagery. This is the objective of laboratory and field exercises as well as the reason behind the production of imagery/map kits. The way in which these learning kits (containing conventional remotely sensed and collateral data products) are put together is described and some concerns that influence the creation of learning kits are discussed. These include budgetary constraints, number of imagery types, and number of collateral data types.

  20. Community Consultation Kit.

    ERIC Educational Resources Information Center

    Boulder Area Growth Study Commission, CO.

    This kit, designed for leaders and participants, provides a model for organizing and taking part in Community Consultation Groups. The kit was designed to be used in connection with community concerns about growth in Boulder, Colorado. These groups build upon a previous survey to assist the Commission in determining specific growth concerns in the…

  1. The Indonesia Kit. A Study Kit.

    ERIC Educational Resources Information Center

    Briere, Elaine; Gage, Susan

    This document is designed for Canadians interested in the South Pacific island chain nation of Indonesia. The kit includes information, photographs, and illustrations concerning Indonesia, West Papua (Irian Jaya), and East Timor. There are discussions of Indonesia's environment, its transmigration program, development refugees, and ties with…

  2. Segregation of Fischer-Tropsch reactants on cobalt nanoparticle surfaces.

    PubMed

    Lewis, E A; Le, D; Jewell, A D; Murphy, C J; Rahman, T S; Sykes, E C H

    2014-06-21

    Using scanning tunnelling microscopy, we have visualized the segregation of carbon monoxide and hydrogen, the two reactants in Fischer-Tropsch synthesis, on cobalt nanoparticles at catalytically relevant coverages. Density functional theory was used to interrogate the relevant energetics.

  3. Acute Phase Reactants as Novel Predictors of Cardiovascular Disease

    PubMed Central

    Ahmed, M. S.; Jadhav, A. B.; Hassan, A.; Meng, Qing H.

    2012-01-01

    Acute phase reaction is a systemic response which usually follows a physiological condition that takes place in the beginning of an inflammatory process. This physiological change usually lasts 1-2 days. However, the systemic acute phase response usually lasts longer. The aim of this systemic response is to restore homeostasis. These events are accompanied by upregulation of some proteins (positive acute phase reactants) and downregulation of others (negative acute phase reactants) during inflammatory reactions. Cardiovascular diseases are accompanied by the elevation of several positive acute phase reactants such as C-reactive protein (CRP), serum amyloid A (SAA), fibrinogen, white blood cell count, secretory nonpancreatic phospholipase 2-II (sPLA2-II), ferritin, and ceruloplasmin. Cardiovascular disease is also accompanied by the reduction of negative acute phase reactants such as albumin, transferrin, transthyretin, retinol-binding protein, antithrombin, and transcortin. In this paper, we will be discussing the biological activity and diagnostic and prognostic values of acute phase reactants with cardiovascular importance. The potential therapeutic targets of these reactants will be also discussed. PMID:24049653

  4. Burn Wise Awareness Kit

    EPA Pesticide Factsheets

    Health and safety outreach materials in the form of an awareness kit. Designed specifically for state, local, and tribal air agencies working to reduce wood smoke pollution, it includes best burn tips, social media m

  5. Cryogenic reactant storage for lunar base regenerative fuel cells

    NASA Technical Reports Server (NTRS)

    Kohout, Lisa L.

    1989-01-01

    There are major advantages to be gained by integrating a cryogenic reactant storage system with a hydrogen-oxygen regenerative fuel cell (RFC) to provide on-site electrical power during the lunar night. Although applicable to any power system using hydrogen-oxygen RFC's for energy storage, cryogenic reactant storage offers a significant benefit whenever the sun/shade cycle and energy storage period approach hundreds of hours. For solar power installations on the moon, cryogenic reactant storage reduces overall specific mass and meteoroid vulnerability of the system. In addition, it offers synergistic benefits to on-site users, such as availability of primary fuel cell reactants for surface rover vehicles and cryogenic propellants for OTV's. The integration involves processing and storing the RFC reactant streams as cryogenic liquids rather than pressurized gases, so that reactant containment (tankage per unit mass of reactants) can be greatly reduced. Hydrogen-oxygen alkaline RFC's, GaAs photovoltaic (PV) arrays, and space cryogenic processing/refrigeration technologies are assumed to be available for the conceptual system design. Advantages are demonstrated by comparing the characteristics of two power system concepts: a conventional lunar surface PV/RFC power system using pressurized gas storage in SOA filament wound pressure vessels and, that same system with gas liquefaction and storage replacing the pressurized storage. Comparisons are made at 20 and 250 kWe. Although cryogenic storage adds a processing plant (drying and liquefaction) to the system plus 30 percent more solar array to provide processing power, the approximate order of magnitude reduction in tankage mass, confirmed by this analysis, results in a reduction in overall total system mass of approximately 50 percent.

  6. Cryogenic reactant storage for lunar base regenerative fuel cells

    NASA Astrophysics Data System (ADS)

    Kohout, Lisa L.

    1989-02-01

    There are major advantages to be gained by integrating a cryogenic reactant storage system with a hydrogen-oxygen regenerative fuel cell (RFC) to provide on-site electrical power during the lunar night. Although applicable to any power system using hydrogen-oxygen RFC's for energy storage, cryogenic reactant storage offers a significant benefit whenever the sun/shade cycle and energy storage period approach hundreds of hours. For solar power installations on the moon, cryogenic reactant storage reduces overall specific mass and meteoroid vulnerability of the system. In addition, it offers synergistic benefits to on-site users, such as availability of primary fuel cell reactants for surface rover vehicles and cryogenic propellants for OTV's. The integration involves processing and storing the RFC reactant streams as cryogenic liquids rather than pressurized gases, so that reactant containment (tankage per unit mass of reactants) can be greatly reduced. Hydrogen-oxygen alkaline RFC's, GaAs photovoltaic (PV) arrays, and space cryogenic processing/refrigeration technologies are assumed to be available for the conceptual system design. Advantages are demonstrated by comparing the characteristics of two power system concepts: a conventional lunar surface PV/RFC power system using pressurized gas storage in SOA filament wound pressure vessels and, that same system with gas liquefaction and storage replacing the pressurized storage. Comparisons are made at 20 and 250 kWe. Although cryogenic storage adds a processing plant (drying and liquefaction) to the system plus 30 percent more solar array to provide processing power, the approximate order of magnitude reduction in tankage mass, confirmed by this analysis, results in a reduction in overall total system mass of approximately 50 percent.

  7. Cryogenic reactant storage for lunar base regenerative fuel cells

    NASA Astrophysics Data System (ADS)

    Kohout, Lisa L.

    There are major advantages to be gained by integrating a cryogenic reactant storage system with a hydrogen-oxygen regenerative fuel cell (RFC) to provide on-site electrical power during the lunar night. Although applicable to any power system using hydrogen-oxygen RFC's for energy storage, cryogenic reactant storage offers a significant benefit whenever the sun/shade cycle and energy storage period approach hundreds of hours. For solar power installations on the moon, cryogenic reactant storage reduces overall specific mass and meteoroid vulnerability of the system. In addition, it offers synergistic benefits to on-site users, such as availability of primary fuel cell reactants for surface rover vehicles and cryogenic propellants for OTV's. The integration involves processing and storing the RFC reactant streams as cryogenic liquids rather than pressurized gases, so that reactant containment (tankage per unit mass of reactants) can be greatly reduced. Hydrogen-oxygen alkaline RFC's, GaAs photovoltaic (PV) arrays, and space cryogenic processing/refrigeration technologies are assumed to be available for the conceptual system design. Advantages are demonstrated by comparing the characteristics of two power system concepts: a conventional lunar surface PV/RFC power system using pressurized gas storage in SOA filament wound pressure vessels and, that same system with gas liquefaction and storage replacing the pressurized storage. Comparisons are made at 20 and 250 kWe. Although cryogenic storage adds a processing plant (drying and liquefaction) to the system plus 30 percent more solar array to provide processing power, the approximate order of magnitude reduction in tankage mass, confirmed by this analysis, results in a reduction in overall total system mass of approximately 50 percent.

  8. Cryogenic reactant storage for lunar base regenerative fuel cells

    NASA Technical Reports Server (NTRS)

    Kohout, Lisa L.

    1989-01-01

    There are major advantages to be gained by integrating a cryogenic reactant storage system with a hydrogen-oxygen regenerative fuel cell (RFC) to provide on-site electrical power during the lunar night. Although applicable to any power system using hydrogen-oxygen RFC's for energy storage, cryogenic reactant storage offers a significant benefit whenever the sun/shade cycle and energy storage period approach hundreds of hours. For solar power installations on the moon, cryogenic reactant storage reduces overall specific mass and meteoroid vulnerability of the system. In addition, it offers synergistic benefits to on-site users, such as availability of primary fuel cell reactants for surface rover vehicles and cryogenic propellants for OTV's. The integration involves processing and storing the RFC reactant streams as cryogenic liquids rather than pressurized gases, so that reactant containment (tankage per unit mass of reactants) can be greatly reduced. Hydrogen-oxygen alkaline RFC's, GaAs photovoltaic (PV) arrays, and space cryogenic processing/refrigeration technologies are assumed to be available for the conceptual system design. Advantages are demonstrated by comparing the characteristics of two power system concepts: a conventional lunar surface PV/RFC power system using pressurized gas storage in SOA filament wound pressure vessels and, that same system with gas liquefaction and storage replacing the pressurized storage. Comparisons are made at 20 and 250 kWe. Although cryogenic storage adds a processing plant (drying and liquefaction) to the system plus 30 percent more solar array to provide processing power, the approximate order of magnitude reduction in tankage mass, confirmed by this analysis, results in a reduction in overall total system mass of approximately 50 percent.

  9. Membrane reactor/separator: A design for bimolecular reactant addition

    SciTech Connect

    Tonkovich, A.L.Y.; Secker, R.B.; Reed, E.L.

    1995-04-01

    A membrane reactor (MBR) is used to investigate the effect of selective reactant addition on series-parallel reaction networks, such as the oxidative dehydrogenation of ethane to ethylene. Ethylene is favored in an oxygen-lean environment, while excess oxygen favors the formation of combustion products. Control of the reactant ratio (ethane to oxygen) is crucial to both the overall selectivity and the hydrocarbon conversion. Traditional reactor designs co-feed the biomolecular reactants at the top of the reactor at some preset feed ratio. The MBR uses a tube (porous alumina membrane) and shell configuration. One reactant is fed at the top of the MBR, which leads to high selectivities; as the oxygen is consumed, it is replenished via downstream permeation to improve the ethane conversion. The MBR and a plug flow reactor (PFR) are evaluated at 600{degrees}C, with identical space velocities, and using a magnesium oxide catalyst doped with samarium oxide. At low to moderate reactant feed ratios, the ethylene yield in the MBR exceeds the PFR by a factor of three, under some conditions. At higher feed ratios, the performance of the PFR nears or exceeds the performance of the MBR.

  10. Fuel Cell Systems for First Lunar Outpost: Reactant Storage Options

    NASA Technical Reports Server (NTRS)

    Nelson, P. A.

    1996-01-01

    A Lunar Surface Power Working Group was formed to review candidate systems for providing power to the first lunar outpost habitat. The working group concluded that the most attractive candidate included a photovoltaic unit, a fuel cell, a regenerator to recycle the reactants, and storage of oxygen and hydrogen gases. Most of the volume (97 percent) and weight (64 percent) are taken up by the reactants and their storage tank. The large volume is difficult to accommodate, and therefore, the working group explored ways of reducing the volume. An alternative approach to providing separate high pressure storage tanks is to use two of the descent stage propellant storage tanks, which would have to be wrapped with graphite fibers to increase their pressure capability. This saves 90 percent of the volume required for storage of fuel cell reactants. Another approach is to use the descent storage propellant tanks for storage of the fuel cell reactants as cryogenic liquids, but his requires a gas liquefication system, increases the solar array by 40 percent, and increases the heat rejection rate by 170 percent compared with storage of reactants as high pressure gases. For a high power system (greater than 20 kW) the larger energy storage requirements would probably favor the cryogenic storage option.

  11. Fuel cell systems for first lunar outpost -- Reactant storage options

    SciTech Connect

    Nelson, P.A.

    1995-06-01

    A Lunar Surface Power Working Group was formed to review candidate systems for providing power to the First Lunar Outpost habitat. The working group met for five days in the fall of 1992 and concluded that the most attractive candidate included a photovoltaic unit, a fuel cell, a regenerator to recycle the reactants, and storage of oxygen and hydrogen gases. Most of the volume (97%) and weight (64%) are taken up by the reactants and their storage tanks. The large volume is difficult to accommodate, and therefore, the working group explored ways of reducing the volume. An alternative approach to providing separate high pressure storage tanks is to use two of the descent stage propellant storage tanks, which would have to be wrapped with graphite fibers to increase their pressure capability. This saves 90% of the volume required for storage of fuel cell reactants. Another approach is to use the descent storage propellant tanks for storage of the fuel cell reactants as cryogenic liquids, but this requires a gas liquefaction system, increases the solar array by 40%, and increases the heat rejection rate by 170% compared with storage of reactants as high pressure gases. For a high power system (>20 kW) the larger energy storage requirement would probably favor the cryogenic storage option.

  12. Method for reacting nongaseous material with a gaseous reactant

    DOEpatents

    Lumpkin, Robert E.; Duraiswamy, Kandaswamy

    1979-03-27

    This invention relates to a new and novel method and apparatus for reacting nongaseous material with a gaseous reactant comprising introducing a first stream containing a nongaseous material into a reaction zone; simultaneously introducing a second stream containing a gaseous reactant into the reaction zone such that the gaseous reactant immediately contacts and reacts with the first stream thereby producing a gaseous product; forming a spiralling vortex within the reaction zone to cause substantial separation of gases, including the gaseous product, from the nongaseous material; forming and removing a third stream from the reaction zone containing the gaseous product which is substantially free of the nongaseous material before a major portion of the gaseous product can react with the nongaseous material; and forming and removing a fourth stream containing the nongaseous material from the reaction zone.

  13. Supply of reactants for Redox bulk energy storage systems

    NASA Technical Reports Server (NTRS)

    Gahn, R. F.

    1978-01-01

    World resources, reserves, production, and costs of reactant materials, iron, chromium, titanium and bromine for proposed redox cell bulk energy storage systems are reviewed. Supplying required materials for multimegawatt hour systems appears to be feasible even at current production levels. Iron and chromium ores are the most abundant and lowest cost of four reactants. Chromium is not a domestic reserve, but redox system installations would represent a small fraction of U.S. imports. Vast quantities of bromine are available, but present production is low and therefore cost is high. Titanium is currently available at reasonable cost, with ample reserves available for the next fifty years.

  14. One more health benefit of blood donation: reduces acute-phase reactants, oxidants and increases antioxidant capacity.

    PubMed

    Yunce, Muharrem; Erdamar, Husamettin; Bayram, Nezihe Asli; Gok, Sumeyye

    2016-11-01

    One of the most important problems in finding blood donors is the inadequacy of volunteer number. To overcome this problem, one of the solutions we suggest is innovating new health benefits of blood donation. The aim of the present study is to investigate the effects of blood donation on oxidative status markers and acute-phase reactants. A total of 96 healthy volunteers were recruited into the study. Blood samples were withdrawn 5 min before and 24 h after the blood donation. Serum nitric oxide, malondialdehyde levels, and activity of superoxide dismutase and myeloperoxidase were measured spectrophotometrically. Serum levels of high-sensitive C-reactive protein and pentraxin-3 as acute-phase reactants were measured by enzyme-linked immunosorbent assay kits. We found statistically significant lower pentraxin-3 and high-sensitive C-reactive protein levels and higher superoxide dismutase activity and nitric oxide level 24 h after blood donation in serum of blood donor when compared with before blood donation. These findings suggest that blood donation affected oxidative status and acute-phase reactants in donors. Blood donation removes oxidants and decreases oxidative stress by elevating antioxidant enzyme such as superoxide dismutase. This is one more health benefit or reason why we should donate blood. Further large-scale studies should evaluate this mechanism and compare the same effect of wet cupping therapy.

  15. Conductive polymer layers to limit transfer of fuel reactants to catalysts of fuel cells to reduce reactant crossover

    SciTech Connect

    Stanis, Ronald J.; Lambert, Timothy N.

    2016-12-06

    An apparatus of an aspect includes a fuel cell catalyst layer. The fuel cell catalyst layer is operable to catalyze a reaction involving a fuel reactant. A fuel cell gas diffusion layer is coupled with the fuel cell catalyst layer. The fuel cell gas diffusion layer includes a porous electrically conductive material. The porous electrically conductive material is operable to allow the fuel reactant to transfer through the fuel cell gas diffusion layer to reach the fuel cell catalyst layer. The porous electrically conductive material is also operable to conduct electrons associated with the reaction through the fuel cell gas diffusion layer. An electrically conductive polymer material is coupled with the fuel cell gas diffusion layer. The electrically conductive polymer material is operable to limit transfer of the fuel reactant to the fuel cell catalyst layer.

  16. Wet in situ transesterification of microalgae using ethyl acetate as a co-solvent and reactant.

    PubMed

    Park, Jeongseok; Kim, Bora; Chang, Yong Keun; Lee, Jae W

    2017-04-01

    This study addresses wet in situ transesterification of microalgae for the production of biodiesel by introducing ethyl acetate as both reactant and co-solvent. Ethyl acetate and acid catalyst are mixed with wet microalgae in one pot and the mixture is heated for simultaneous lipid extraction and transesterification. As a single reactant and co-solvent, ethyl acetate can provide higher FAEE yield and more saccharification of carbohydrates than the case of binary ethanol and chloroform as a reactant and a co-solvent. The optimal yield was 97.8wt% at 114°C and 4.06M catalyst with 6.67mlEtOAC/g dried algae based on experimental results and response surface methodology (RSM). This wet in situ transesterification of microalgae using ethyl acetate doesn't require an additional co-solvent and it also promises more economic benefit as combining extraction and transesterification in a single process. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Travel Medical Kit.

    PubMed

    Terry, Anne C; Haulman, N Jean

    2016-03-01

    "The traveler's medical kit is an essential tool for both the novice and expert traveler. It is designed to treat travel-related illness and injury and to ensure preexisting medical conditions are managed appropriately. Travelers are at increased risk for common gastrointestinal issues during travel. Respiratory illnesses make up approximately 8% of the ailments present in returned international travelers. Approximately 12% of travelers experience a travel-related skin condition. First aid treatment for minor injuries is essential to all travel medical kits. The complexity ranges from a small, simple case for the urban traveler to a larger, extensive case for wilderness travel."

  18. Reactant pressure differential control for fuel cell gases

    NASA Technical Reports Server (NTRS)

    Grasso, A. P. (Inventor)

    1985-01-01

    A pair of spool valves are described which are balanced between pressures of reactant gases supplied to a fuel cell power plant. The pressure differences are controlled between the gases so as to maintain those pressures substantially in the proportions necessary for operation of the fuel cell.

  19. Optimization of retroviral gene transduction of mobilized primitive hematopoietic progenitors by using thrombopoietin, Flt3, and Kit ligands and RetroNectin culture.

    PubMed

    Murray, L; Luens, K; Tushinski, R; Jin, L; Burton, M; Chen, J; Forestell, S; Hill, B

    1999-07-20

    We have investigated the ability of several cytokine combinations to improve retrovirus-mediated transduction of human primitive hematopoietic progenitors (PHPs) from mobilized peripheral blood (MPB). Retroviral infection of CD34+ cells was performed by culture on fibronectin fragment CH-296 (RetroNectin, RN), using the truncated human nerve growth factor receptor (NGFR) as the transgene reporter. Transgene expression among progeny of PHPs was assayed by FACS analysis after long-term stromal culture (LTC). Transgene delivery to PHPs was assessed by PCR of individual stromal culture-derived methylcellulose colonies (LTC-CFCs). Compared with interleukin 3 (IL-3), IL-6, and leukemia inhibitory factor (LIF), the combination of thrombopoietin (TPO), Flt3 ligand (FL), and Kit ligand (KL) effected a 73-fold increase in NGFR expression among CD34+ cells (to 14%) and a 14-fold increase in NGFR expression among total cells (to 10%) after LTC. In addition, a 2.4-fold increase in neo gene marking of LTC-CFCs was observed. A preclinical study comparing the effect of high-speed centrifugation ("spinoculation") or culture on RN during exposure to retroviral particles in teflon cell culture bags showed no difference in the efficiency of transduction of PHPs between these two methods.

  20. User Authentication. SPEC Kit.

    ERIC Educational Resources Information Center

    Plum, Terry, Comp.; Bleiler, Richard, Comp.

    2001-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit presents the results of a survey of Association of Research Libraries (ARL) member libraries designed to examine the systems research libraries use to authenticate and authorize the users of their online networked information resources. A total of 52 of 121 ARL member libraries responded to…

  1. World Disarmament Kit.

    ERIC Educational Resources Information Center

    Woito, Robert, Ed.

    This kit presents a comprehensive introduction for students to arms control and disarmament issues. Included are copies of published and unpublished articles for each topic. Section I provides a self-survey to enable students to assess their own attitudes, values, and knowledge. The survey poses questions for which students select one of several…

  2. User Authentication. SPEC Kit.

    ERIC Educational Resources Information Center

    Plum, Terry, Comp.; Bleiler, Richard, Comp.

    2001-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit presents the results of a survey of Association of Research Libraries (ARL) member libraries designed to examine the systems research libraries use to authenticate and authorize the users of their online networked information resources. A total of 52 of 121 ARL member libraries responded to…

  3. Ohio EPA Teachers Kit.

    ERIC Educational Resources Information Center

    Ohio State Environmental Protection Agency, Columbus.

    In an effort to provide teachers in Ohio with assistance in environmental education, the Ohio Environmental Protection Agency (EPA) has produced this teachers kit. It is designed to describe what the Ohio EPA is doing to protect Ohio's air, land, and water. The background information provides an historical account of some of the events that have…

  4. Balloons and Science Kit.

    ERIC Educational Resources Information Center

    Balloon Council, Washington, DC.

    This document provides background information on balloons including: (1) the history of balloons; (2) balloon manufacturing; (3) biodegradability; (4) the fate of latex balloons; and (5) the effect of balloons on the rainforest and sea mammals. Also included as part of this instructional kit are four fun experiments that allow students to…

  5. Personalized Thematic Kits

    ERIC Educational Resources Information Center

    Bontrager, Sharon

    2010-01-01

    Teaching Spanish at the K-5 level is a passion of mine, and the author would like to share some of the practical applications that she finds most rewarding and effective. She has found enthusiastic response to the creation of detailed language learning kits that are rooted in storytelling, but expanded to include home-made board games,…

  6. [Conference Time Kit.

    ERIC Educational Resources Information Center

    National School Public Relations Association, Washington, DC.

    This multimedia kit, for use with and by teachers from kindergarten through the upper elementary grades, consists of four components: 1) a filmstrip for teachers; 2) the 1970 edition of a handbook, "Conference Time for Teachers and Parents"; 3) a filmstrip for parents; 4) a supporting parent information leaflet "How To Confer Successfully with…

  7. Theme Kits Made Easy.

    ERIC Educational Resources Information Center

    Eslinger, Leslie Silk

    Recognizing the long-lasting impact of young childrens learning through themes as well as the amount of teacher time spent in preparing for this type of teaching, this kit is designed to help teachers avoid the shortcomings of theme-based teaching, while capitalizing on the benefits of this approach. The book is presented in two sections. The…

  8. Theme Kits Made Easy.

    ERIC Educational Resources Information Center

    Eslinger, Leslie Silk

    Recognizing the long-lasting impact of young childrens learning through themes as well as the amount of teacher time spent in preparing for this type of teaching, this kit is designed to help teachers avoid the shortcomings of theme-based teaching, while capitalizing on the benefits of this approach. The book is presented in two sections. The…

  9. Projectable Basic Electronics Kit.

    ERIC Educational Resources Information Center

    H'ng, John; And Others

    1982-01-01

    Outlines advantages derived from constructing and using a Projectable Basic Electronics Kit and provides: (1) list of components; (2) diagrams of 10 finished components (resistor; capacitor; diode; switch; bulb; transistor; meter; variable capacitor; coil; connecting terminal); and (3) diode and transistor activities. (JN)

  10. Leisure Counseling. A Kit.

    ERIC Educational Resources Information Center

    Epperson, Arlin; And Others

    This set of materials intended for use in the development of programs in leisure services and a vocational counseling contains information about a Leisure Counseling Media Kit, with directions for ordering a slide-tape program. Order forms and additional information about leisure counseling supplies are also included. A brief pamphlet describes…

  11. Projectable Basic Electronics Kit.

    ERIC Educational Resources Information Center

    H'ng, John; And Others

    1982-01-01

    Outlines advantages derived from constructing and using a Projectable Basic Electronics Kit and provides: (1) list of components; (2) diagrams of 10 finished components (resistor; capacitor; diode; switch; bulb; transistor; meter; variable capacitor; coil; connecting terminal); and (3) diode and transistor activities. (JN)

  12. Early Childhood Kits.

    ERIC Educational Resources Information Center

    National Center on Educational Media and Materials for the Handicapped, Columbus, OH.

    Selected from the National Instructional Materials Information System (NIMIS)--a computer based on-line interactive retrieval system on special education materials, the bibliography covers 80 kits for developing skills at the early childhood level. Entries are presented in order of NIMIS accession number and include the following information:…

  13. Core Competencies. SPEC Kit.

    ERIC Educational Resources Information Center

    McNeil, Beth, Comp.

    2002-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit presents the results of a survey of Association of Research Libraries (ARL) member libraries designed to investigate the status of core competencies (i.e., the skills, knowledge, abilities, and attributes that employees across an organization are expected to have to contribute successfully…

  14. Vietnamese Culture Kit.

    ERIC Educational Resources Information Center

    Nguyen, Liem Thanh

    This booklet provides a brief description of the cultural background of the Vietnamese, the geography of the country of Vietnam, the history of the Vietnamese people, their language, beliefs, systems of values, religions, customs, feasts, and holidays. The kit is designed to provide American sponsors and teachers with meaningful information about…

  15. Voter Education Training Kit.

    ERIC Educational Resources Information Center

    Multi-District Inst. for Political Education, Pitman, NJ.

    Guides and resources in this kit are prepared for a six week to two month secondary voter education course. The objectives are to prepare and motivate eligible students to register and vote in the presidential election, to participate in the presidential election campaigning, and to increase their overall knowledge concerning the presidential…

  16. The ESL Starter Kit.

    ERIC Educational Resources Information Center

    Virginia Commonwealth Univ., Richmond. Virginia Adult Education and Literacy Resource Center.

    The kit is intended for teachers beginning to teach English as a Second Language (ESL). The first part offers some ideas for testing, registering, and placing students according to their needs and goals. A sample registration form, placement test, list of commercially-available tests, and sample needs assessments are included here. The second…

  17. Ohio EPA Teachers Kit.

    ERIC Educational Resources Information Center

    Ohio State Environmental Protection Agency, Columbus.

    In an effort to provide teachers in Ohio with assistance in environmental education, the Ohio Environmental Protection Agency (EPA) has produced this teachers kit. It is designed to describe what the Ohio EPA is doing to protect Ohio's air, land, and water. The background information provides an historical account of some of the events that have…

  18. Chat Reference. SPEC Kit.

    ERIC Educational Resources Information Center

    Ronan, Jana, Comp.; Turner, Carol, Comp.

    2002-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit presents the results of a survey of Association of Research Libraries (ARL) member libraries designed to gather data on chat reference service. A total of 66 of 124 ARL member libraries responded to the survey. A copy of the questionnaire with tabulated results is presented. Representative…

  19. [Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique].

    PubMed

    Li, Cheng-Tao; Guo, Hong; Lin, Yuan; Liu, Yan; Que, Ting-Zhi; Li, Li

    2008-04-01

    To explore the appropriate amount of template DNA for Sinofiler Kit. The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay. It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit. Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.

  20. Combustion flame-plasma hybrid reactor systems, and chemical reactant sources

    DOEpatents

    Kong, Peter C

    2013-11-26

    Combustion flame-plasma hybrid reactor systems, chemical reactant sources, and related methods are disclosed. In one embodiment, a combustion flame-plasma hybrid reactor system comprising a reaction chamber, a combustion torch positioned to direct a flame into the reaction chamber, and one or more reactant feed assemblies configured to electrically energize at least one electrically conductive solid reactant structure to form a plasma and feed each electrically conductive solid reactant structure into the plasma to form at least one product is disclosed. In an additional embodiment, a chemical reactant source for a combustion flame-plasma hybrid reactor comprising an elongated electrically conductive reactant structure consisting essentially of at least one chemical reactant is disclosed. In further embodiments, methods of forming a chemical reactant source and methods of chemically converting at least one reactant into at least one product are disclosed.

  1. Automatic transmission adapter kit

    SciTech Connect

    Stich, R.L.; Neal, W.D.

    1987-02-10

    This patent describes, in a four-wheel-drive vehicle apparatus having a power train including an automatic transmission and a transfer case, an automatic transmission adapter kit for installation of a replacement automatic transmission of shorter length than an original automatic transmission in the four-wheel-drive vehicle. The adapter kit comprises: an extension housing interposed between the replacement automatic transmission and the transfer case; an output shaft, having a first end which engages the replacement automatic transmission and a second end which engages the transfer case; first sealing means for sealing between the extension housing and the replacement automatic transmission; second sealing means for sealing between the extension housing and the transfer case; and fastening means for connecting the extension housing between the replacement automatic transmission and the transfer case.

  2. Space processing applications payload equipment study. Volume 2D: SPA supplemental power and heat rejection kit

    NASA Technical Reports Server (NTRS)

    Hammel, R. L. (Editor); Smith, A. G. (Editor)

    1974-01-01

    The design and application of a supplementary power and heat rejection kit for the Spacelab are discussed. Two subsystems of electric power and thermal control were analyzed to define the requirements for the power and heat rejection kit (PHRK). Twelve exemplary experiments were defined and power timelines were developed. From these timeline, the experiment requirements for sustained power, peak power, and energy were determined. The electrical power subsystem of the PHRK will consist of two fuel cells, oxygen and hydrogen reactant tank assemblies, water storage tanks, plumbing, cabling, and inverters to convert the nominal 28 volt dc fuel cell output to ac power.

  3. 49 CFR 173.161 - Chemical kits and first aid kits.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 2 2013-10-01 2013-10-01 false Chemical kits and first aid kits. 173.161 Section... Class 7 § 173.161 Chemical kits and first aid kits. (a) Applicability. Chemical kits and first aid kits...) The kits may only contain hazardous materials for which packaging exceptions are provided in column...

  4. 49 CFR 173.161 - Chemical kits and first aid kits.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 2 2011-10-01 2011-10-01 false Chemical kits and first aid kits. 173.161 Section... Class 7 § 173.161 Chemical kits and first aid kits. (a) Applicability. Chemical kits and first aid kits...) The kits may only contain hazardous materials for which packaging exceptions are provided in column...

  5. 49 CFR 173.161 - Chemical kits and first aid kits.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 2 2014-10-01 2014-10-01 false Chemical kits and first aid kits. 173.161 Section... Class 7 § 173.161 Chemical kits and first aid kits. (a) Applicability. Chemical kits and first aid kits...) The kits may only contain hazardous materials for which packaging exceptions are provided in column...

  6. 49 CFR 173.161 - Chemical kits and first aid kits.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 2 2012-10-01 2012-10-01 false Chemical kits and first aid kits. 173.161 Section... Class 7 § 173.161 Chemical kits and first aid kits. (a) Applicability. Chemical kits and first aid kits...) The kits may only contain hazardous materials for which packaging exceptions are provided in column...

  7. Molecular defects in mastocytosis: KIT and beyond KIT.

    PubMed

    Bibi, Siham; Langenfeld, Florent; Jeanningros, Sylvie; Brenet, Fabienne; Soucie, Erinn; Hermine, Olivier; Damaj, Gandhi; Dubreuil, Patrice; Arock, Michel

    2014-05-01

    In all variants of mastocytosis, activating KIT mutations are frequently found. In adults, neoplastic mast cells (MCs) cells show the KIT mutation D816V, whereas in children, MCs invading the skin are frequently positive for non-KIT D816V mutations. The clinical course and prognosis of the disease vary among patients with systemic mastocytosis (SM). Additional KIT-independent molecular defects might cause progression. Additional oncogenic lesions have recently been identified in advanced SM. In advanced SM the presence of additional genetic lesions or altered signaling worsening the prognosis might lead to the use of alternative therapies such as combined antisignaling targeted treatments or stem cell transplantation.

  8. Telescience Resource Kit

    NASA Technical Reports Server (NTRS)

    Schneider, Michelle; Lippincott, Jeff; Chubb, Steve; Whitaker, Jimmy; Rice, Jim; Gillis, Robert; Sims, Chris; Sellers, Donna; Bailey, Darrell (Technical Monitor)

    2002-01-01

    The Telescience Resource Kit (TReK) is a PC based ground control system. It can be used by a single individual or in a group environment to monitor and control spacecraft systems and payloads. Capabilities include data receipt, data processing, data storage, data management, and data transmission. Commercial-Off-The-Shelf (COTS) hardware and software have been employed to reduce development costs, operations and maintenance costs, and to effectively take advantage of new commercial products as they become available. The TReK system is currently being used to monitor and control payloads aboard the International Space Station. It is located at sites around the world.

  9. Telescience Resource Kit

    NASA Technical Reports Server (NTRS)

    Schneider, Michelle; Lippincott, Jeff; Chubb, Steve; Whitaker, Jimmy; Rice, Jim; Gillis, Robert; Sims, Chris; Sellers, Donna; Bailey, Darrell (Technical Monitor)

    2002-01-01

    The Telescience Resource Kit (TReK) is a PC based ground control system. It can be used by a single individual or in a group environment to monitor and control spacecraft systems and payloads. Capabilities include data receipt, data processing, data storage, data management, and data transmission. Commercial-Off-The-Shelf (COTS) hardware and software have been employed to reduce development costs, operations and maintenance costs, and to effectively take advantage of new commercial products as they become available. The TReK system is currently being used to monitor and control payloads aboard the International Space Station. It is located at sites around the world.

  10. KIT — EDRN Public Portal

    Cancer.gov

    SCF-sR, also known as KIT, is the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. Human KIT is a tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

  11. Addition-type polyimides from solutions of monomeric reactants.

    NASA Technical Reports Server (NTRS)

    Delvigs, P.; Serafini, T. T.; Lightsey, G. R.

    1972-01-01

    The monomeric reactants approach was used to fabricate addition-type polyimide/graphite fiber composites with improved mechanical properties and thermal stability characteristics over those of composites derived from addition-type amide acid prepolymers. A screening study of 24 different monomer combinations was performed. The results of a more extensive investigation of a selected number of monomer combinations showed that the combination providing the best thermomechanical properties was 5-norbornene-2,3-dicarboxylic acid monomethyl ester/4,4'-methylenedianiline/3,3'4,4'-benzophenonetetracarboxylic acid dimethyl ester at a molar ratio of 2/3.09/2.09.

  12. Electrochemical devices utilizing molten alkali metal electrode-reactant

    DOEpatents

    Hitchcock, D.C.; Mailhe, C.C.; De Jonghe, L.C.

    1985-07-10

    Electrochemical cells are provided with a reactive metal to reduce the oxide of the alkali metal electrode-reactant. Cells employing a molten alkali metal electrode, e.g., sodium, in contact with a ceramic electrolyte, which is a conductor of the ions of the alkali metal forming the electrode, exhibit a lower resistance when a reactive metal, e.g., vanadium, is allowed to react with and reduce the alkali metal oxide. Such cells exhibit less degradation of the electrolyte and of the glass seals often used to joining the electrolyte to the other components of the cell under cycling conditions.

  13. Structure, bonding, and reactivity of reactant complexes and key intermediates.

    PubMed

    Soriano, Elena; Marco-Contelles, José

    2011-01-01

    Complexes of Pt and Au (gold(III) and cationic gold(I)) have shown an exceptional ability to promote a variety of organic transformations of unsaturated precursors due to their peculiar Lewis acid properties: the alkynophilic character of these soft metals and the π-acid activation of unsaturated groups promotes the intra- or intermolecular attack of a nucleophile. In this chapter we summarize the computational data reported on the structure, bonding, and reactivity of the reactant π-complexes and also on the key intermediate species.

  14. Addition-type polyimides from solutions of monomeric reactants

    NASA Technical Reports Server (NTRS)

    Delvigs, P.; Serafini, T. T.; Lightsey, G. R.

    1972-01-01

    The monomeric reactants approach was used to fabricate addition-type polyimide/graphite fiber composites with improved mechanical properties and thermal stability characteristics over those of composites derived from addition-type amide acid prepolymers. A screening study of 24 different monomer combinations was performed. The results of a more extensive investigation of a selected number of monomer combinations showed that the combination providing the best thermomechanical properties was 5-norbornene-2,3-dicarboxylic acid monomethyl ester/4,4'-methylenedianiline/3,3'4,4'-benzophenone tetracarboxylic acid dimethyl ester at a molar ratio of 2/3.09/2.09.

  15. Addition-type polyimides from solutions of monomeric reactants

    NASA Technical Reports Server (NTRS)

    Delvigs, P.; Serafini, T. T.; Lightsey, G. R.

    1972-01-01

    The monomeric reactants approach was used to fabricate addition-type polyimide/graphite fiber composites with improved mechanical properties and thermal stability characteristics over those of composites derived from addition-type amide acid prepolymers. A screening study of 24 different monomer combinations was performed. The results of a more extensive investigation of a selected number of monomer combinations showed that the combination providing the best thermomechanical properties was 5-norbornene-2.3-dicarboxylic acid monomethyl ester/4,4(')-methylenedianiline/3,3(')4,4(')-benzophenonetetracarboxylic acid dimethyl ester at a molar ratio of 2/3.09/2.09.

  16. Electrochemical cell utilizing molten alkali metal electrode-reactant

    DOEpatents

    Virkar, Anil V.; Miller, Gerald R.

    1983-11-04

    An improved electrochemical cell comprising an additive-modified molten alkali metal electrode-reactant and/or electrolyte is disclosed. Various electrochemical cells employing a molten alkali metal, e.g., sodium, electrode in contact with a cationically conductive ceramic membrane experience a lower resistance and a lower temperature coefficient of resistance whenever small amounts of selenium are present at the interface of the electrolyte and the molten alkali metal. Further, cells having small amounts of selenium present at the electrolyte-molten metal interface exhibit less degradation of the electrolyte under long term cycling conditions.

  17. Electrochemical devices utilizing molten alkali metal electrode-reactant

    DOEpatents

    Hitchcock, David C.; Mailhe, Catherine C.; De Jonghe, Lutgard C.

    1986-01-01

    Electrochemical cells are provided with a reactive metal to reduce the oxide of the alkali metal electrode-reactant. Cells employing a molten alkali metal electrode, e.g., sodium, in contact with a ceramic electrolyte, which is a conductor of the ions of the alkali metal forming the electrode, exhibit a lower resistance when a reactive metal, e.g., vanadium, is allowed to react with and reduce the alkali metal oxide. Such cells exhibit less degradation of the electrolyte and of the glass seals often used to joining the electrolyte to the other components of the cell under cycling conditions.

  18. Optics learning through affordable kit

    SciTech Connect

    P, Anusha N E-mail: chitrashaji@gmail.com Shaji, Chitra E-mail: chitrashaji@gmail.com Sharan, Alok E-mail: chitrashaji@gmail.com

    2014-10-15

    An affordable kit which helps to understand some of the optical phenomena qualitatively and quantitatively is presented in this paper. It supplements optics taught in classes. The kit consists of equipments which are available in the market at nominal cost such as laser pointer, lenses, glass plates, razor blades, coins, ball bearing etc. Experiments which come under wave optics (interference and diffraction) and ray optics (reflection and refraction) are explained using this kit.

  19. Education Payload Operation - Kit D

    NASA Technical Reports Server (NTRS)

    Keil, Matthew

    2009-01-01

    Education Payload Operation - Kit D (EPO-Kit D) includes education items that will be used to support the live International Space Station (ISS) education downlinks and Education Payload Operation (EPO) demonstrations onboard the ISS. The main objective of EPO-Kit D supports the National Aeronautics and Space Administration (NASA) goal of attracting students to study and seek careers in science, technology, engineering, and mathematics.

  20. A Hands-On Activity Incorporating the Threefold Representation on Limiting Reactant

    ERIC Educational Resources Information Center

    Gonza´lez-Sa´nchez, Ange´lica M.; Ortiz-Nieves, Edgardo L.; Medina, Zuleikra

    2014-01-01

    Many students share the common belief that the limiting reactant in a chemical reaction is the reactant in the smallest quantity of material. To help students overcome this difficulty a hands-on activity for the limiting reactant concept was developed. The activity incorporates the three levels of representation (macroscopic, submicroscopic, and…

  1. A Hands-On Activity Incorporating the Threefold Representation on Limiting Reactant

    ERIC Educational Resources Information Center

    Gonza´lez-Sa´nchez, Ange´lica M.; Ortiz-Nieves, Edgardo L.; Medina, Zuleikra

    2014-01-01

    Many students share the common belief that the limiting reactant in a chemical reaction is the reactant in the smallest quantity of material. To help students overcome this difficulty a hands-on activity for the limiting reactant concept was developed. The activity incorporates the three levels of representation (macroscopic, submicroscopic, and…

  2. Effect of thermal expansion on the stability of two-reactant flames

    NASA Technical Reports Server (NTRS)

    Jackson, T. L.

    1986-01-01

    The full problem of flame stability for the two-reactant model, which takes into account thermal expansion effects for all disturbance wave lengths, is examined. It is found that the stability problem for the class of two-reactant flames is equivalent to the stability problem for the class of one-reactant flames with an appropriate interpretation of Lewis numbers.

  3. Reactant Carrier Microfoam Technology for In-Situ Remediation of Radionuclide and Metallic Contaminants in Deep Vadose Zone

    SciTech Connect

    Mattigod, Shas V.; Zhong, Lirong; Jansik, Danielle P.; Foote, Martin; Hart, Andrea T.; Wellman, Dawn M.

    2010-07-01

    The U.S. Department of Energy (DOE) is currently developing advanced remedial technologies for addressing metal and radionuclide (Cr, Tc, and U) contamination in deep vadose zone environments. One of the transformational technology alternatives being considered by the DOE Office of Environmental Management, is the use of Reactant Carrier Microfoams (RCM) as a minimally invasive method for delivery and emplacement of reagents for in-situ immobilization of contaminants. Penetration of low permeability zones deep within the subsurface for Enhance Oil Recovery (EOR) has been well-established. Use of surfactant foams have also been explored for mobilizing DNAPL from sediments. So far, the concept of using RCM for immobilizing labile metal and long-lived radionuclide contaminants in the deep vadose zone has not been explored. We, at the Pacific Northwest National Laboratory (PNNL), conducted studies to develop stable foams as a means to deliver reductive and/or precipitating reactants to the deep subsurface. To test the feasibility of this approach, we developed a preliminary foam formulation consisting of a mixture of an anionic and a nonionic surfactant with a reactant consisting of a 9:1 blend of tripoly- and orthophosphate. The MSE Technology Applications, Inc (MSE) in collaboration with PNNL, conducted a scale-up test to evaluate the efficacy of this reactant carrier foam for in-situ immobilization of U containing sediment zones in a heterogenous sediment matrix. The data indicated that successful immobilization of U contamination is feasible using specifically tailored reactant carrier foam injection technology. Studies are continuing for developing more robust optimized RCM for highly mobile contaminants such as Cr (VI), Tc (VII) in the deep vadose zone.

  4. Modeling reaction fronts of separated condensed phase reactants

    NASA Astrophysics Data System (ADS)

    Koundinyan, Sushilkumar; Stewart, D. Scott; Matalon, Moshe

    2017-01-01

    We present a Gibbs free energy approach to modeling reaction fronts in condensed phase reactive materials. The current interest is in chemical reactions of condensed phase reactants that are initially separated. In energetic materials such reactions are observed to occur extremely fast and at relatively sharp fronts. The condensed phase combustion process differs in several aspects from classical gaseous combustion due to the disparity between the characteristic thermal conductivity length and the mass diffusion lengths and a volume, temperature, stress, mass fraction equation of state that principally depends only on the component reference volumes and the current mixture composition. To retain a simple planar configuration, we consider the two reactants, in solid phase, are in motion towards each other characterized by counter-flow geometry. We apply the model to a simplified Titanium-Boron system and present the analysis of reaction zone length for various strain rates. The numerical results are validated with asymptotic approximations at the Burke-Schumann (complete combustion) limit.

  5. Modeling reaction fronts of separated condensed phase reactants

    NASA Astrophysics Data System (ADS)

    Koundinyan, Sushilkumar; Matalon, Moshe; Stewart, D. Scott; Bdzil, John

    2015-06-01

    We present a Gibbs free energy approach to modeling reaction fronts in condensed phase reactive materials. The current interest is in chemical reactions of condensed phase reactants that are initially separated. In energetic materials such reactions are observed to occur extremely fast and at relatively sharp fronts. The solid-to-solid combustion process differs in several aspects from classical gaseous combustion due to the disparity between the characteristic thermal conductivity length and the mass diffusion lengths and a volume, temperature, stress, mass fraction equation of state that principally depends only on the component reference volumes and the current mixture composition. To retain a simple planar configuration, we consider the two reactants, in solid phase, are in motion towards each other characterized by counter-flow geometry. We apply the model to a simplified Titanium-Boron system and present the analysis of reaction zone length for various strain rates. The numerical results are validated with asymptotic approximations at the Burke-Schumann limit. Supported by HDTRA1-10-1-0020 (DTRA), AF Sub MO C00039417-1 (AFOSR/TRE).

  6. Method of preparing electrodes with porous current collector structures and solid reactants for secondary electrochemical cells

    DOEpatents

    Gay, Eddie C.; Martino, Fredric J.

    1976-01-01

    Particulate electrode reactants, for instance transition metal sulfides for the positive electrodes and lithium alloys for the negative electrodes, are vibratorily compacted into porous, electrically conductive structures. Structures of high porosity support sufficient reactant material to provide high cell capacity per unit weight while serving as an electrical current collector to improve the utilization of reactant materials. Pore sizes of the structure and particle sizes of the reactant material are selected to permit uniform vibratory loading of the substrate without settling of the reactant material during cycling.

  7. Method of synthesizing a plurality of reactants and producing thin films of electro-optically active transition metal oxides

    DOEpatents

    Tracy, C.E.; Benson, D.K.; Ruth, M.R.

    1985-08-16

    A method of synthesizing a plurality of reactants by inducing a reaction by plasma deposition among the reactants. The plasma reaction is effective for consolidating the reactants and producing thin films of electro-optically active transition metal oxides.

  8. Workshop Training Kits. Volume II.

    ERIC Educational Resources Information Center

    Ward, Ted; And Others

    Presented in the second of a two volume series are six workshop training kits for development of teacher skills to be used with learning disabled (LD) children. The first section of each kit contains a leader's guide which gives activity, objectives, teacher prerequisites, time required, materials needed, step-by-step procedures, a discussion…

  9. Organization Charts. SPEC Kit 1.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This kit, containing organization charts for 15 member libraries of the Association of Research Libraries (ARL), is a revised and updated version of a 1973 kit. In addition to charts of the total organization, many institutions have supplied charts for specific departments such as circulation, acquisitions, catalog, business, and technical…

  10. Improve Quality: Use Tool Kits.

    ERIC Educational Resources Information Center

    Gartner, Sue

    2001-01-01

    Addresses issues of defining quality in both business and community service. Describes the use of a regulatory tool kit containing rules and regulations a child care center must follow to ensure children's health, safety, and well-being. Specific tool kit types described include regulatory, government funded, rating scale, and NAEYC. (SD)

  11. International Literacy Day Tool Kit.

    ERIC Educational Resources Information Center

    2002

    This tool kit suggests various International Literacy Day activities to raise awareness of the issues of adult literacy and language learning, to connect local literacy programs with national programs, and to help achieve the National Literacy Summit goal by 2010. The kit is intended for individuals, programs, and organizations that want to call…

  12. Planning Systems. SPEC Kit 13.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This kit on planning systems updates a 1974 Management Studies Office Systems and Procedures Exchange Center (SPEC) kit in which developments in planning activities among Association of Research Libraries (ARL) were reviewed. At the time of the original study, in 1972, planning techniques and systems were a subject of much interest because rising…

  13. First Follow Nature, Kit II.

    ERIC Educational Resources Information Center

    1971

    Developing pupils' awareness of their environment, learning to distinguish between what is pleasant and unpleasant, and examining acts of man to determine which are destructive and which are in harmony with nature are the purposes of Scholastic's Earth Corps Environmental Study Kits for Grades 1-6. This kit explores in depth the reasons some…

  14. Look Around You, Kit I.

    ERIC Educational Resources Information Center

    1971

    Developing pupils' awareness of their environment, learning to distinguish between what is pleasant and unpleasant, and examining acts of man to determine which are destructive and which are in harmony with nature are the purposes of Scholastic's Earth Corps Environmental Study Kits for grades 1-6. This kit is designed to help the child develop…

  15. Affirmative Action. SPEC Kit 4.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This Systems and Procedures Exchange Center (SPEC) kit on affirmative action programs contains planning documents and policy statements on affirmative action and equal employment opportunity from universities and Association of Research Libraries (ARL) member libraries. The kit comprises: (1) "Affirmative Action Plan for the University Library"…

  16. Improve Quality: Use Tool Kits.

    ERIC Educational Resources Information Center

    Gartner, Sue

    2001-01-01

    Addresses issues of defining quality in both business and community service. Describes the use of a regulatory tool kit containing rules and regulations a child care center must follow to ensure children's health, safety, and well-being. Specific tool kit types described include regulatory, government funded, rating scale, and NAEYC. (SD)

  17. Calculating fusion neutron energy spectra from arbitrary reactant distributions

    NASA Astrophysics Data System (ADS)

    Eriksson, J.; Conroy, S.; Andersson Sundén, E.; Hellesen, C.

    2016-02-01

    The Directional Relativistic Spectrum Simulator (DRESS) code can perform Monte-Carlo calculations of reaction product spectra from arbitrary reactant distributions, using fully relativistic kinematics. The code is set up to calculate energy spectra from neutrons and alpha particles produced in the D(d, n)3He and T(d, n)4He fusion reactions, but any two-body reaction can be simulated by including the corresponding cross section. The code has been thoroughly tested. The kinematics calculations have been benchmarked against the kinematics module of the ROOT Data Analysis Framework. Calculated neutron energy spectra have been validated against tabulated fusion reactivities and against an exact analytical expression for the thermonuclear fusion neutron spectrum, with good agreement. The DRESS code will be used as the core of a detailed synthetic diagnostic framework for neutron measurements at the JET and MAST tokamaks.

  18. Space shuttle electrical power generation and reactant supply system

    NASA Technical Reports Server (NTRS)

    Simon, W. E.

    1985-01-01

    The design philosophy and development experience of fuel cell power generation and cryogenic reactant supply systems are reviewed, beginning with the state of technology at the conclusion of the Apollo Program. Technology advancements span a period of 10 years from initial definition phase to the most recent space transportation system (STS) flights. The development program encompassed prototype, verification, and qualification hardware, as well as post-STS-1 design improvements. Focus is on the problems encountered, the scientific and engineering approaches employed to meet the technological challenges, and the results obtained. Major technology barriers are discussed, and the evolving technology development paths are traced from their conceptual beginnings to the fully man-rated systems which are now an integral part of the shuttle vehicle.

  19. A Java commodity grid kit.

    SciTech Connect

    von Laszewski, G.; Foster, I.; Gawor, J.; Lane, P.; Mathematics and Computer Science

    2001-07-01

    In this paper we report on the features of the Java Commodity Grid Kit. The Java CoG Kit provides middleware for accessing Grid functionality from the Java framework. Java CoG Kit middleware is general enough to design a variety of advanced Grid applications with quite different user requirements. Access to the Grid is established via Globus protocols, allowing the Java CoG Kit to communicate also with the C Globus reference implementation. Thus, the Java CoG Kit provides Grid developers with the ability to utilize the Grid, as well as numerous additional libraries and frameworks developed by the Java community to enable network, Internet, enterprise, and peer-to peer computing. A variety of projects have successfully used the client libraries of the Java CoG Kit to access Grids driven by the C Globus software. In this paper we also report on the efforts to develop server side Java CoG Kit components. As part of this research we have implemented a prototype pure Java resource management system that enables one to run Globus jobs on platforms on which a Java virtual machine is supported, including Windows NT machines.

  20. Influence of the reactant carbon-hydrogen-oxygen composition on the key products of the direct gasification of dewatered sewage sludge in supercritical water.

    PubMed

    Gong, Miao; Zhu, Wei; Fan, Yujie; Zhang, Huiwen; Su, Ying

    2016-05-01

    The supercritical water gasification of ten different types of dewatered sewage sludges was investigated to understand the relationship between sludge properties and gasification products. Experiments were performed in a high-pressure autoclave at 400°C for 60 min. Results showed that gasification of sewage sludge in supercritical water consists mainly of a gasification reaction, a carbonization reaction and a persistent organic pollutants synthesis reaction. Changes in the reactant C/H/O composition have significant effects on the key gasification products. Total gas production increased with increasing C/H2O of the reactant. The char/coke content increased with increasing C/H ratio of the reactant. A decrease in the C/O ratio of the reactant led to a reduction in polycyclic aromatic hydrocarbon formation. This means that we can adjust the reactant C/H/O composition by adding carbon-, hydrogen-, and oxygen-containing substances such as coal, algae and H2O2 to optimize hydrogen production and to inhibit an undesired by-product formation. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Application Kit for Federal Assistance

    EPA Pesticide Factsheets

    The Federal Grant & Cooperative Agreement Act of 1977 requires Federal agencies to use a contract to acquire property or services that directly benefit the Federal government.This letter to the applicant explains the Application Kit for Federal Assistance.

  2. Build an Emergency Preparedness Kit

    MedlinePlus

    ... tire traction -Red or brightly- colored cloth -NOAA weather radio For more information on building emergency kits, ... and a flashlight with extra batteries. A NOAA weather radio warns the public of severe weather and ...

  3. Energy Management Curriculum Starter Kit

    SciTech Connect

    Turner, W.C.

    1987-02-01

    The Energy Management Curriculum Starter Kit was designed to help engineering educators develop and teach energy management courses. Montana State University and Oklahoma State University courses are embodied in the model curriculum given. The curricula offered at many other universities throughout the United States are also presented. The kit was designed specifically to train engineering students to be good energy managers. Courses at both the undergraduate and postgraduate level are presented.

  4. Microfoams as Reactant Transport Media for In-Situ Immobilization of Radionuclide and Metallic Contaminants in Deep Vadose Zone

    NASA Astrophysics Data System (ADS)

    Wellman, D. M.; Zhong, L.; Mattigod, S.; Jansik, D.

    2009-12-01

    The U.S. Department of Energy (DOE) is currently addressing issues related to remediation of Cr, U and Tc contamination in the deep vadose zone at the Hanford Site in Washington State. One of the transformational technology alternatives being considered by the DOE Office of Environmental Management, is the use of Reactant Carrier Microfoams (RCM) for in-situ immobilization of contaminants. Foam injection technology for Enhance Oil Recovery (EOR) has well-established pedigree. Use of surfactant foams have also been explored for mobilizing DNAPL from sediments. However, the novel concept of using RCM for in situ immobilization contaminants in the deep vadose zone has not been explored, therefore, presents many daunting challenges for successful implementation. Scienists at Pacific Northwest National Laboratory (PNNL), leveraged previous EMSP-funded studies on microfoams conducted at LBNL with the goal to formulate robust stable microfoams for delivering reductive and/or precipitating reactants to the deep subsurface. Following an extensive literature review, a protocol was deisnged to select appropriate surfactant blends, and tested three different methods of foam generation namely, Venturi foam generato , high-speed gas entrainment and porous plate method. The resulting RCMs were characterized as to their quality, stability, bubble size distribution, surface tension and viscosity. The foam stabilities as a function of reactant (polyphosphate and polysulfides) concentrations and entrained polyatomic gases were also examined. Based on these experiments, optimal carrier foam compositions were identified for each Hanford deep vadose zone Contaminant of Concern (COC) namely U(VI) and Cr(VI). Finally, MSE Technology Applications, Inc (MSE) in collaboration with PNNL, conducted a series of scale-up reactant carrier foam injection tests to evaluate the efficacy of this technology for potential deep vadose zone remediation.

  5. Very high frequency plasma reactant for atomic layer deposition

    NASA Astrophysics Data System (ADS)

    Oh, Il-Kwon; Yoo, Gilsang; Yoon, Chang Mo; Kim, Tae Hyung; Yeom, Geun Young; Kim, Kangsik; Lee, Zonghoon; Jung, Hanearl; Lee, Chang Wan; Kim, Hyungjun; Lee, Han-Bo-Ram

    2016-11-01

    Although plasma-enhanced atomic layer deposition (PE-ALD) results in several benefits in the formation of high-k dielectrics, including a low processing temperature and improved film properties compared to conventional thermal ALD, energetic radicals and ions in the plasma cause damage to layer stacks, leading to the deterioration of electrical properties. In this study, the growth characteristics and film properties of PE-ALD Al2O3 were investigated using a very-high-frequency (VHF) plasma reactant. Because VHF plasma features a lower electron temperature and higher plasma density than conventional radio frequency (RF) plasma, it has a larger number of less energetic reaction species, such as radicals and ions. VHF PE-ALD Al2O3 shows superior physical and electrical properties over RF PE-ALD Al2O3, including high growth per cycle, excellent conformality, low roughness, high dielectric constant, low leakage current, and low interface trap density. In addition, interlayer-free Al2O3 on Si was achieved in VHF PE-ALD via a significant reduction in plasma damage. VHF PE-ALD will be an essential process to realize nanoscale devices that require precise control of interfaces and electrical properties.

  6. The adaptor 3BP2 is required for KIT receptor expression and human mast cell survival

    PubMed Central

    Ainsua-Enrich, Erola; Serrano-Candelas, Eva; Álvarez-Errico, Damiana; Picado, César; Sayós, Joan; Rivera, Juan; Martín, Margarita

    2015-01-01

    3BP2 is a cytoplasmic adaptor protein that acts as a positive regulator in mast cell FcεRI-dependent signaling. The KIT receptor whose ligand is the stem cell factor (SCF) is necessary for mast cell development, proliferation and survival as well as for optimal IgE-dependent signal. Activating mutations in KIT have been associated with several diseases including mastocytosis. In the present work, we found that 3BP2 silencing impairs KIT signaling pathways, thus affecting PI3K and MAP kinase pathways in human mast cells from HMC-1, LAD2 (human mast cell lines) and CD34+-derived mast cells. Unexpectedly, silencing of 3BP2 reduces KIT expression in normal human mast cells as well as in HMC-1 cells where KIT is mutated, thus increasing cellular apoptosis and caspase 3/7 activity. 3BP2 silencing reduces KIT transcription expression levels. Interestingly, 3BP2 silencing decreased MITF expression, a transcription factor involved in KIT expression. Reconstitution of 3BP2 in knockdown cells leads to reversal of KIT expression as well as survival phenotype. Accordingly MITF reconstitution enhances KIT expression levels in 3BP2 silenced cells. Moreover, downregulation of KIT expression by miRNA221 overexpression or the proteasome inhibitor bortezomib also reduced 3BP2 and MITF expression. Furthermore, KIT tyrosine activity inhibition reduced 3BP2 and MITF expression, demonstrating again a tight and reciprocal relationship between these molecules. Taken together, our results show that 3BP2 regulates human mast cell survival and participates in KIT-mediated signal transduction by directly controlling KIT receptor expression, suggesting its potential as a therapeutic target in mast cell-mediated inflammatory diseases and deregulated KIT disorders. PMID:25810396

  7. Identification of a novel subgroup of melanomas with KIT/cyclin-dependent kinase-4 overexpression.

    PubMed

    Smalley, Keiran S M; Contractor, Rooha; Nguyen, Thiennga K; Xiao, Min; Edwards, Robin; Muthusamy, Viswanathan; King, Alastair J; Flaherty, Keith T; Bosenberg, Marcus; Herlyn, Meenhard; Nathanson, Katherine L

    2008-07-15

    Although many melanomas harbor either activating mutations in BRAF or NRAS, there remains a substantial, yet little known, group of tumors without either mutation. Here, we used a genomic strategy to define a novel group of melanoma cell lines with co-overexpression of cyclin-dependent kinase 4 (CDK4) and KIT. Although this subgroup lacked any known KIT mutations, they had high phospho-KIT receptor expression, indicating receptor activity. Quantitative PCR confirmed the existence of a similar KIT/CDK4 subgroup in human melanoma samples. Pharmacologic studies showed the KIT/CDK4-overexpressing subgroup to be resistant to BRAF inhibitors but sensitive to imatinib in both in vitro and in vivo melanoma models. Mechanistically, imatinib treatment led to increased apoptosis and G(1) phase cell cycle arrest associated with the inhibition of phospho-ERK and increased expression of p27(KIP). Other melanoma cell lines, which retained some KIT expression but lacked phospho-KIT, were not sensitive to imatinib, suggesting that KIT expression alone is not predictive of response. We suggest that co-overexpression of KIT/CDK4 is a potential mechanism of oncogenic transformation in some BRAF/NRAS wild-type melanomas. This group of melanomas may be a subpopulation for which imatinib or other KIT inhibitors may constitute optimal therapy.

  8. General theory of multistage geminate reactions of isolated pairs of reactants. I. Kinetic equations

    NASA Astrophysics Data System (ADS)

    Doktorov, Alexander B.; Kipriyanov, Alexey A.

    2014-05-01

    General matrix approach to the consideration of multistage geminate reactions of isolated pairs of reactants depending on reactant mobility is formulated on the basis of the concept of "effective" particles. Various elementary reactions (stages of multistage reaction including physicochemical processes of internal quantum state changes) proceeding with the participation of isolated pairs of reactants (or isolated reactants) are taken into account. Investigation has been made in terms of kinetic approach implying the derivation of general (matrix) kinetic equations for local and mean probabilities of finding any of the reaction species in the sample under study (or for local and mean concentrations). The recipes for the calculation of kinetic coefficients of the equations for mean quantities in terms of relative coordinates of reactants have been formulated in the general case of inhomogeneous reacting systems. Important specific case of homogeneous reacting systems is considered.

  9. General theory of multistage geminate reactions of isolated pairs of reactants. I. Kinetic equations

    SciTech Connect

    Doktorov, Alexander B.; Kipriyanov, Alexey A.

    2014-05-14

    General matrix approach to the consideration of multistage geminate reactions of isolated pairs of reactants depending on reactant mobility is formulated on the basis of the concept of “effective” particles. Various elementary reactions (stages of multistage reaction including physicochemical processes of internal quantum state changes) proceeding with the participation of isolated pairs of reactants (or isolated reactants) are taken into account. Investigation has been made in terms of kinetic approach implying the derivation of general (matrix) kinetic equations for local and mean probabilities of finding any of the reaction species in the sample under study (or for local and mean concentrations). The recipes for the calculation of kinetic coefficients of the equations for mean quantities in terms of relative coordinates of reactants have been formulated in the general case of inhomogeneous reacting systems. Important specific case of homogeneous reacting systems is considered.

  10. Solid phase microextraction field kit

    DOEpatents

    Nunes, Peter J.; Andresen, Brian D.

    2005-08-16

    A field kit for the collection, isolation and concentration of trace amounts of high explosives (HE), biological weapons (BW) and chemical weapons (CW) residues in air, soil, vegetation, swipe, and liquid samples. The field kit includes a number of Solid Phase Microextraction (SPME) fiber and syringe assemblies in a hermetically sealed transportation container or tubes which includes a sampling port, a number of extra SPME fiber and syringe assemblies, the fiber and syringe assemblies including a protective cap for the fiber, and an extractor for the protective cap, along with other items including spare parts, protective glove, and an instruction manual, all located in an airtight container.

  11. Comparison of DNA extraction kits for detection of Burkholderia pseudomallei in spiked human whole blood using real-time PCR.

    PubMed

    Podnecky, Nicole L; Elrod, Mindy G; Newton, Bruce R; Dauphin, Leslie A; Shi, Jianrong; Chawalchitiporn, Sutthinan; Baggett, Henry C; Hoffmaster, Alex R; Gee, Jay E

    2013-01-01

    Burkholderia pseudomallei, the etiologic agent of melioidosis, is endemic in northern Australia and Southeast Asia and can cause severe septicemia that may lead to death in 20% to 50% of cases. Rapid detection of B. pseudomallei infection is crucial for timely treatment of septic patients. This study evaluated seven commercially available DNA extraction kits to determine the relative recovery of B. pseudomallei DNA from spiked EDTA-containing human whole blood. The evaluation included three manual kits: the QIAamp DNA Mini kit, the QIAamp DNA Blood Mini kit, and the High Pure PCR Template Preparation kit; and four automated systems: the MagNAPure LC using the DNA Isolation Kit I, the MagNAPure Compact using the Nucleic Acid Isolation Kit I, and the QIAcube using the QIAamp DNA Mini kit and the QIAamp DNA Blood Mini kit. Detection of B. pseudomallei DNA extracted by each kit was performed using the B. pseudomallei specific type III secretion real-time PCR (TTS1) assay. Crossing threshold (C T ) values were used to compare the limit of detection and reproducibility of each kit. This study also compared the DNA concentrations and DNA purity yielded for each kit. The following kits consistently yielded DNA that produced a detectable signal from blood spiked with 5.5×10(4) colony forming units per mL: the High Pure PCR Template Preparation, QIAamp DNA Mini, MagNA Pure Compact, and the QIAcube running the QIAamp DNA Mini and QIAamp DNA Blood Mini kits. The High Pure PCR Template Preparation kit yielded the lowest limit of detection with spiked blood, but when this kit was used with blood from patients with confirmed cases of melioidosis, the bacteria was not reliably detected indicating blood may not be an optimal specimen.

  12. Comparison of DNA Extraction Kits for Detection of Burkholderia pseudomallei in Spiked Human Whole Blood Using Real-Time PCR

    PubMed Central

    Podnecky, Nicole L.; Elrod, Mindy G.; Newton, Bruce R.; Dauphin, Leslie A.; Shi, Jianrong; Chawalchitiporn, Sutthinan; Baggett, Henry C.; Hoffmaster, Alex R.; Gee, Jay E.

    2013-01-01

    Burkholderia pseudomallei, the etiologic agent of melioidosis, is endemic in northern Australia and Southeast Asia and can cause severe septicemia that may lead to death in 20% to 50% of cases. Rapid detection of B. pseudomallei infection is crucial for timely treatment of septic patients. This study evaluated seven commercially available DNA extraction kits to determine the relative recovery of B. pseudomallei DNA from spiked EDTA-containing human whole blood. The evaluation included three manual kits: the QIAamp DNA Mini kit, the QIAamp DNA Blood Mini kit, and the High Pure PCR Template Preparation kit; and four automated systems: the MagNAPure LC using the DNA Isolation Kit I, the MagNAPure Compact using the Nucleic Acid Isolation Kit I, and the QIAcube using the QIAamp DNA Mini kit and the QIAamp DNA Blood Mini kit. Detection of B. pseudomallei DNA extracted by each kit was performed using the B. pseudomallei specific type III secretion real-time PCR (TTS1) assay. Crossing threshold (CT) values were used to compare the limit of detection and reproducibility of each kit. This study also compared the DNA concentrations and DNA purity yielded for each kit. The following kits consistently yielded DNA that produced a detectable signal from blood spiked with 5.5×104 colony forming units per mL: the High Pure PCR Template Preparation, QIAamp DNA Mini, MagNA Pure Compact, and the QIAcube running the QIAamp DNA Mini and QIAamp DNA Blood Mini kits. The High Pure PCR Template Preparation kit yielded the lowest limit of detection with spiked blood, but when this kit was used with blood from patients with confirmed cases of melioidosis, the bacteria was not reliably detected indicating blood may not be an optimal specimen. PMID:23460920

  13. Instructional Support Services. SPEC Kit.

    ERIC Educational Resources Information Center

    Snyder, Carolyn A., Comp.; Logue, Susan, Comp.; Carter, Howard, Comp.; Soltys, Mickey, Comp.

    2001-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit presents the results of a survey of Association of Research Libraries (ARL) member libraries. The survey was designed to gather information about the changes that have taken place in the last five years in the services libraries offer to support instruction on campus and in the library, as…

  14. Extended Library Hours. SPEC Kit.

    ERIC Educational Resources Information Center

    Steele, Patricia Ann, Comp.; Walters, Carolyn, Comp.

    2001-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit presents the results of a survey of Association of Research Libraries (ARL) member libraries designed to provide a description of how they are responding to demands for greater hours of access and service. Survey responses indicate what hours of access and service libraries are providing and…

  15. Natural Gas Energy Educational Kit.

    ERIC Educational Resources Information Center

    American Gas Association, Arlington, VA. Educational Services.

    Prepared by energy experts and educators to introduce middle school and high school students to natural gas and its role in our society, this kit is designed to be incorporated into existing science and social studies curricula. The materials and activities focus on the origin, discovery, production, delivery, and use of natural gas. The role of…

  16. Sharing the Earth, Kit II.

    ERIC Educational Resources Information Center

    1971

    Introducing the pupil to the science of ecology is the purpose of Scholastic's Earth Corps Ecology/Conservation Study Kits for grades 3-6. Simple terms are used to show how all living things are inter-related to their environment, to demonstrate the intricate and delicate balance of nature, and to point out how man's interference with nature's…

  17. Human Conflict: A Resource Kit.

    ERIC Educational Resources Information Center

    J.B. Speed Art Museum, Louisville, KY.

    Conflict, in one form or another, eventually makes its way into the daily life of every human being. It is not just a modern problem but has existed as long as humanity itself. This classroom resource kit is designed to be used by educators wishing to teach skills of conflict avoidance, management, and resolution in their classrooms. The kit…

  18. Small Business Incubator Resource Kit.

    ERIC Educational Resources Information Center

    Small Business Administration, Washington, DC.

    This kit consists of a set of resources to assist those interested in the start-up and management (incubation) of a new business. A guide to starting and managing a small business incubator (SBI) is provided. Included in the guide are the following: a discussion of the role and characteristics of the SBI concept; guidelines for carrying out the…

  19. User Surveys. SPEC Kit 148.

    ERIC Educational Resources Information Center

    Engelbrecht, Pamela Noyes

    Based on responses to a survey of Association of Research Libraries (ARL) members in March 1988, this Systems and Procedures Exchange Center (SPEC) flyer and kit are designed to assist administrators of large academic libraries in the selection of useful methods of conducting user surveys for particular library concerns. The flyer provides a brief…

  20. Organization Charts. SPEC Kit 129.

    ERIC Educational Resources Information Center

    Hoadley, Irene B.

    This kit is based on a review of the organization charts of 86 member libraries of the Association of Research Libraries (ARL) gathered in spring 1986, compared with two earlier sets of charts published in 1973 and 1977, and contains charts of 61 of the libraries. Nearly one-fourth of the charts studied are under revision, indicating that changes…

  1. Natural Gas Energy Educational Kit.

    ERIC Educational Resources Information Center

    American Gas Association, Arlington, VA. Educational Services.

    Prepared by energy experts and educators to introduce middle school and high school students to natural gas and its role in our society, this kit is designed to be incorporated into existing science and social studies curricula. The materials and activities focus on the origin, discovery, production, delivery, and use of natural gas. The role of…

  2. Sharing the Earth, Kit II.

    ERIC Educational Resources Information Center

    1971

    Introducing the pupil to the science of ecology is the purpose of Scholastic's Earth Corps Ecology/Conservation Study Kits for grades 3-6. Simple terms are used to show how all living things are inter-related to their environment, to demonstrate the intricate and delicate balance of nature, and to point out how man's interference with nature's…

  3. Adult College Completion Tool Kit

    ERIC Educational Resources Information Center

    Tolbert, Michelle

    2012-01-01

    This "Adult College Completion Tool Kit" is designed to connect state administrators and local practitioners to the strategies, resources, and technical assistance tools resulting from the Department's work. States can use this information to identify and implement state adult education leadership priorities, supported by federal "Adult Education…

  4. Johnny Horizon Environmental Test Kit.

    ERIC Educational Resources Information Center

    Bentley, Richard; Bentley, William

    Derived from tests presently used by state and federal agencies involved with pollution detection, this Environmental Test Kit contains materials and instructions for ten experiments. Each experiment is designed to test a different aspect of air and water, to find out whether or not the air and water in the tester's immediate area has been…

  5. Work and Family Resource Kit.

    ERIC Educational Resources Information Center

    Women's Bureau (DOL), Washington, DC.

    This kit is designed to help employers understand the range of family needs emerging in the workplace and the numerous options for a company response. An introduction discusses the need for child care services, dependent care problems, and how employers respond and benefit. Sections address the following: selecting the right option in relation to…

  6. Collection Assessment. SPEC Kit 41.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This Association of Research Libraries (ARL) kit on collection assessment contains the following documents: (1) "Guidelines for the Evaluation of Library Collections--Draft Copy" (Collection Development Committee, Resource Section, Resources and Technical Services Division, American Library Association); (2) "Guidelines for Collection Assessment"…

  7. Developing the Advising "Tool Kit."

    ERIC Educational Resources Information Center

    Kelly, James J.

    1988-01-01

    Developing an inventory consisting of each and every item that contributes to a total advising program is suggested. The point to be made by developing a campus "Advising Tool Kit" such as Penn State's is that advising and advisors are becoming increasingly integral to the entire educational mission of colleges and universities. (MLW)

  8. Architectural Environment: A Resource Kit.

    ERIC Educational Resources Information Center

    J.B. Speed Art Museum, Louisville, KY.

    There are many ways to approach the investigation of architecture. One can look at structural form, climate and topography, the aesthetics of style and decoration, building function, historical factors, cultural meanings, or technology and techniques associated with construction. This resource kit touches upon a few of these approaches, ranging…

  9. Approval Plans. SPEC Kit 141.

    ERIC Educational Resources Information Center

    Howard, Clinton

    This SPEC kit is based on a June 1987 survey of approval plan use by 94 members of the Association of Research Libraries. It reports on the current practices of these libraries, the perceived advantages and disadvantages of using approval plans, and the effect of increased library materials costs and library automation on such plans. It is noted…

  10. Skills Training. SPEC Kit 40.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    A 1977 Association of Research Libraries (ARL) survey investigated how libraries were training personnel for routine tasks. Of the 73 responding libraries, more than 75% (58) reported that manuals and procedural guides, along with on-the-job training, were the main methods of instruction for technical skills. Accordingly, this kit on skills…

  11. Active Parenting Now: Program Kit.

    ERIC Educational Resources Information Center

    Popkin, Michael H.

    Based largely on the theories of Alfred Adler and Rudolf Dreikurs, this parent education curriculum is a video-based interactive learning experience that teaches a comprehensive model of parenting to parents of children ages 5 to 12 years. The kit provides parents with the skills needed to help their children develop courage, responsibility, and…

  12. Higher Education Salary Evaluation Kit.

    ERIC Educational Resources Information Center

    Scott, Elizabeth L.

    The kit is the result of a study undertaken to provide a method for flagging women and minority faculty members whose salaries appear to be low compared to the salaries of white males in the same faculty who have the same attributes and experience. The recommended method also provides an estimate of what the woman's or minority individual's salary…

  13. Architectural Environment: A Resource Kit.

    ERIC Educational Resources Information Center

    J.B. Speed Art Museum, Louisville, KY.

    There are many ways to approach the investigation of architecture. One can look at structural form, climate and topography, the aesthetics of style and decoration, building function, historical factors, cultural meanings, or technology and techniques associated with construction. This resource kit touches upon a few of these approaches, ranging…

  14. Collection Assessment. SPEC Kit 41.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This Association of Research Libraries (ARL) kit on collection assessment contains the following documents: (1) "Guidelines for the Evaluation of Library Collections--Draft Copy" (Collection Development Committee, Resource Section, Resources and Technical Services Division, American Library Association); (2) "Guidelines for Collection Assessment"…

  15. Performance Review. SPEC Kit 14.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This kit contains a collection of representative documents on performance review procedures in academic and research libraries. Multiple documents from the Pennsylvania State University Libraries include a management guide to performance evaluation, a memo on performance evaluation from the librarian, and forms and instructions for use of both the…

  16. Skills Training. SPEC Kit 40.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    A 1977 Association of Research Libraries (ARL) survey investigated how libraries were training personnel for routine tasks. Of the 73 responding libraries, more than 75% (58) reported that manuals and procedural guides, along with on-the-job training, were the main methods of instruction for technical skills. Accordingly, this kit on skills…

  17. Care Bears Environmental Awareness Kit.

    ERIC Educational Resources Information Center

    American Greetings Corp., Cleveland, OH.

    Studies show that the three most frequently cited sources of environmental information are family, school, and the media. This kit provides parents with an opportunity to increase a child's environmental awareness through activities which focus on the environment in a way children ages four to nine can understand. A workbook uses the popular Care…

  18. Johnny Horizon Environmental Test Kit.

    ERIC Educational Resources Information Center

    Bentley, Richard; Bentley, William

    Derived from tests presently used by state and federal agencies involved with pollution detection, this Environmental Test Kit contains materials and instructions for ten experiments. Each experiment is designed to test a different aspect of air and water, to find out whether or not the air and water in the tester's immediate area has been…

  19. Active Parenting Now: Program Kit.

    ERIC Educational Resources Information Center

    Popkin, Michael H.

    Based largely on the theories of Alfred Adler and Rudolf Dreikurs, this parent education curriculum is a video-based interactive learning experience that teaches a comprehensive model of parenting to parents of children ages 5 to 12 years. The kit provides parents with the skills needed to help their children develop courage, responsibility, and…

  20. Cubic Unit Cell Construction Kit.

    ERIC Educational Resources Information Center

    Mattson, Bruce

    2000-01-01

    Presents instructions for building a simple interactive unit-cell construction kit that allows for the construction of simple, body-centered, and face-centered cubic lattices. The lit is built from inexpensive and readily available materials and can be built in any number of sizes. (WRM)

  1. Evaluation of America's Career Kit.

    ERIC Educational Resources Information Center

    Office of Inspector General (DOL), Washington, DC.

    The Employment and Training Administration's (ETA's) development and implementation of America's Career Kit (ACK), which is an online career development resource for individuals needing job search assistance, career guidance, salary data, and training and educational resources, was evaluated. The evaluation was designed to determine whether ACK…

  2. The effects of CO2 on the negative reactant ions of IMS

    NASA Technical Reports Server (NTRS)

    Spangler, Glenn E.

    1995-01-01

    In the presence of CO2, the negative reactant ions of ion mobility spectrometry (IMS) are ion clusters of CO4(-) and CO3(-). Methyl salicylate is ionized by the CO4(-)(H2O(n))(N2(m)) reactant ions, but not by the CO3(-)(H2O(n))(N2(m)) reactant ions. While the CO4(-) ions are formed by direct association, the CO3(-) ions require additional energy to be formed. The additional energy is provided by either excited neutral gas molecules in a metastable state or UV (ultraviolet) radiation.

  3. Supersaturation control in aragonite synthesis using sparingly soluble calcium sulfate as reactants.

    PubMed

    Hu, Zeshan; Deng, Yulin

    2003-10-15

    Sparingly soluble calcium salts were studied as reactants in the synthesis of needle-like precipitated calcium carbonate (PCC). The morphology and aspect ratio of the PCC particles were characterized with SEM. Polymorphs and crystal size were characterized using X-ray diffraction. The counterions of the sparingly soluble salts influenced the growth kinetics of PCC as well as the polymorphism and morphology of product particles. Either chrysanthemum-like or needle-like aragonite can be synthesized from calcium sulfate and sodium carbonate depending on the supersaturation and synthesis conditions. Low concentration and slow addition rate of sodium carbonate solution were favorable to the formation of aragonite. Addition of sodium sulfate to the reaction system (calcium chloride and sodium carbonate) promoted the formation of aragonite and decreased the crystal size of aragonite due to the decrease of supersaturation and adsorption of sulfate ion. Too much added sodium sulfate, however, did not further increase the aragonite fraction. An optimal temperature for the formation of aragonite was found to be ca. 60 degrees C. Slow dissolution kinetics of sparingly dissoluble salt also is very important for controlling PCC polymorphism and morphology.

  4. Plasma-free atomic layer deposition of Ru thin films using H{sub 2} molecules as a nonoxidizing reactant

    SciTech Connect

    Lee, Seung-Joon; Kim, Soo-Hyun; Saito, Masayuki; Suzuki, Kazuharu; Nabeya, Shunichi; Lee, Jeongyeop; Kim, Sangdeok; Yeom, Seungjin; Lee, Do-Joong

    2016-05-15

    The ruthenium (Ru) thin films were grown by atomic layer deposition (ALD) using a sequential supply of dicarbonyl-bis(5-methyl-2,4-hexanediketonato) Ru(II) (C{sub 16}H{sub 22}O{sub 6}Ru) and H{sub 2} as a reactant at a substrate temperature of 250 °C. Deposition was possible using H{sub 2} molecules without a plasma by increasing the chamber pressure to above 10 Torr. Specifically, high-quality Ru films with a low resistivity of ∼40 μΩ cm and few amount of oxygen (∼1.2 at. %) were obtained under a chamber pressure of 300 Torr though the oxygen was contained in the precursor. Under the optimized conditions, self-limited film growth with regard to the precursor and reactant pulsing times was confirmed under elevated chamber pressures. The ALD-Ru process proposed in this study showed one of the highest growth rates of 0.12 nm/cycle on a thermally grown SiO{sub 2} substrate, as well as a very low number of incubation cycles (approximately 12 cycles). Cross-sectional view transmission electron microscopy showed that no interfacial oxide had formed during the deposition of the ALD-Ru films on a W surface using H{sub 2} molecules, whereas ∼7 nm thick interfacial oxide was formed when O{sub 2} molecules were used as a reactant. The step coverage of the ALD-Ru film onto very small-sized trenches (aspect ratio: ∼4.5 and the top opening size of 25 nm) and holes (aspect ratio: ∼40 and top opening size of 40 nm) was excellent (∼100%).

  5. Basic Teaching Kit on Consumer Advertising.

    ERIC Educational Resources Information Center

    Proctor and Gamble Co., Cincinnati, OH.

    This advertising kit was developed by Procter and Gamble in response to requests from teachers and consumer educators who asked for materials from business about business. The kit is not intended to cover the entire field of advertising. Rather, it centers on advertising as it is known and practiced by Procter and Gamble. The purpose of the kit is…

  6. 47 CFR 15.25 - Kits.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 1 2012-10-01 2012-10-01 false Kits. 15.25 Section 15.25 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL RADIO FREQUENCY DEVICES General § 15.25 Kits. A TV interface device, including a cable system terminal device, which is marketed as a kit shall comply with...

  7. 47 CFR 15.25 - Kits.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 1 2013-10-01 2013-10-01 false Kits. 15.25 Section 15.25 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL RADIO FREQUENCY DEVICES General § 15.25 Kits. A TV interface device, including a cable system terminal device, which is marketed as a kit shall comply with...

  8. 47 CFR 15.25 - Kits.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Kits. 15.25 Section 15.25 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL RADIO FREQUENCY DEVICES General § 15.25 Kits. A TV interface device, including a cable system terminal device, which is marketed as a kit shall comply with...

  9. 47 CFR 15.25 - Kits.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 1 2014-10-01 2014-10-01 false Kits. 15.25 Section 15.25 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL RADIO FREQUENCY DEVICES General § 15.25 Kits. A TV interface device, including a cable system terminal device, which is marketed as a kit shall comply with...

  10. 47 CFR 15.25 - Kits.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 1 2011-10-01 2011-10-01 false Kits. 15.25 Section 15.25 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL RADIO FREQUENCY DEVICES General § 15.25 Kits. A TV interface device, including a cable system terminal device, which is marketed as a kit shall comply with...

  11. Migration Oz, An Investigative Resource Kit.

    ERIC Educational Resources Information Center

    History Teachers' Association of Victoria (Australia).

    This resource kit examines immigration and population issues in Australia. Many organizations with an active interest in immigration policy are represented in the kit, giving students the opportunity to evaluate alternative positions and views. The kit reflects the Commonwealth Government's multicultural policies and the goals that underlie them,…

  12. Basic Teaching Kit on Consumer Advertising.

    ERIC Educational Resources Information Center

    Proctor and Gamble Co., Cincinnati, OH.

    This advertising kit was developed by Procter and Gamble in response to requests from teachers and consumer educators who asked for materials from business about business. The kit is not intended to cover the entire field of advertising. Rather, it centers on advertising as it is known and practiced by Procter and Gamble. The purpose of the kit is…

  13. 21 CFR 876.5210 - Enema kit.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Enema kit. 876.5210 Section 876.5210 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES GASTROENTEROLOGY-UROLOGY DEVICES Therapeutic Devices § 876.5210 Enema kit. (a) Identification. An enema kit is a...

  14. Measuring D(d,p)T fusion reactant energy spectra with Doppler shifted fusion products

    SciTech Connect

    Boris, D. R.; Kulcinski, G. L.; Santarius, J. F.; Donovan, D. C.; Piefer, G. R.

    2010-06-15

    Deuterium fusion reactant energy spectra have been measured using a diagnostic that records the Doppler shift imparted to charged particle fusion products of the D(d,p)T reaction by the center-of-mass velocity of the deuterium reactants. This diagnostic, known as the fusion ion Doppler shift diagnostic (FIDO) measures fast deuterium energy spectra in the inertial electrostatic confinement (IEC) experiment at the University of Wisconsin-Madison {l_brace}Santarius et al. [Fusion Sci. Technol. 47, 1238 (2005)]{r_brace}, a device to confine high energy light ions in a spherically symmetric, electrostatic potential well. This article details the first measurements of the fusion reactant energy spectra in an IEC device as well as the design and principles of operation of the FIDO diagnostic. Scaling of reactant energy spectra with a variety of experimental parameters have been explored.

  15. Effects of reactant rotational excitation on reactivity: Perspectives from the sudden limit

    SciTech Connect

    Jiang, Bin; Li, Jun; Guo, Hua

    2014-01-21

    Excitation of reactant rotational degrees of freedom is known to influence reactivity in bimolecular reactions. In this work, this effect is examined for several prototypical activated atom-diatom and atom-triatom reactions through exact quantum scattering calculations on accurate ab initio potential energy surfaces. To rationalize these mode-specific effects, the recently proposed sudden vector overlap model is extended to include rotational motions of reactants. Specifically, the enhancement of reactivity is attributed to their coupling with the reaction coordinate at the transition state, as quantified by the alignment between the corresponding normal mode vectors. In addition, a Franck-Condon model is introduced to predict the effect of reactant rotational excitation for reactions in which the reactant rotations are decoupled from the reaction coordinate.

  16. Comparison of three DNA extraction kits to establish maximum yield and quality of coral-associated microbial DNA

    USGS Publications Warehouse

    Baker, Erin J.; Kellogg, Christina A.

    2014-01-01

    Coral microbiology is an expanding field, yet there is no standard DNA extraction protocol. Although many researchers depend on commercial extraction kits, no specific kit has been optimized for use with coral samples. Both soil and plant DNA extraction kits from MO BIO Laboratories, Inc., have been used by many research groups for this purpose. MO BIO recently replaced their PowerPlant® kit with an improved PowerPlantPro kit, but it was unclear how these changes would affect the kit’s use with coral samples. In order to determine which kit produced the best results, we conducted a comparison between the original PowerPlant kit, the new PowerPlantPro kit, and an alternative kit, PowerSoil, using samples from several different coral genera. The PowerPlantPro kit had the highest DNA yields, but the lack of 16S rRNA gene amplification in many samples suggests that much of the yield may be coral DNA rather than microbial DNA. The most consistent positive amplifications came from the PowerSoil kit.

  17. Reactant ion chemistry for detection of TNT, RDX, and PETN using an ion mobility spectrometer

    SciTech Connect

    Klassen, S.E.; Rodacy, P.; Silva, R.

    1997-09-01

    This report describes the responses of three energetic materials (TNT, RDX, and PETN) to varying reactant ion chemistries and IMS cell temperatures. The following reactant ion chemistries were evaluated; air-dry; air-wet; methylene chloride-dry; methylene chloride-wet; methylene bromide-dry; nitrogen dioxide-wet; sulfur dioxide-wet. The temperature was varied between 160 - 220{degrees}C.

  18. Method of synthesizing a plurality of reactants and producing thin films of electro-optically active transition metal oxides

    DOEpatents

    Tracy, C. Edwin; Benson, David K.; Ruth, Marta R.

    1987-01-01

    A method of synthesizing electro-optically active reaction products from a plurality of reactants by inducing a reaction by plasma deposition among the reactants. The plasma reaction is effective for consolidating the reactants and producing thin films of electro-optically active transition metal oxides.

  19. The Quality Lighting Teaching Kit: enlightening our future

    NASA Astrophysics Data System (ADS)

    Walker, Constance E.; Pompea, Stephen M.

    2016-09-01

    The U.S. National Optical Astronomy Observatory's Education and Public Outreach group has produced a Quality Lighting Teaching (QLT) Kit, as an outcome of the International Year of Light 2015. The kits are designed around problem-based learning scenarios. The kit's six activities allow students to address real lighting problems that relate to wildlife, sky glow, aging eyes, energy consumption, safety, and light trespass. The activities are optimized for 11-14 year olds but can be expanded to younger and older. Most of the activities can be done within in a few minutes with the exception of the Energy Activity. The activities can be done during class or afterschool and as stations (that the students rotate through) or as stand-alones (one at a time). All aspects of the program are as ready-for-use. Everything you need for the six activities is included in the kit. Tutorial videos (on the program's webpage) have been created on how to do the activities. They can be found on the webpage, www.noao.edu/education/qltkit.php. Fourteen Google+ Hangouts on Air have been offered, addressing questions on the activities and logistics. Assessments (in the form of pre- and post-surveys for the students and as post-surveys for the instructors) provide learning outcomes and improvements. Eighty-nine out of 100 kits have been distributed to SPIE, OSA, CIE, IDA and the IAU in 31 countries. The QLT Kit is a stepping-stone to bring awareness to the (younger) public on how quality lighting locally can redress issues like light pollution globally.

  20. StochKit2: software for discrete stochastic simulation of biochemical systems with events.

    PubMed

    Sanft, Kevin R; Wu, Sheng; Roh, Min; Fu, Jin; Lim, Rone Kwei; Petzold, Linda R

    2011-09-01

    StochKit2 is the first major upgrade of the popular StochKit stochastic simulation software package. StochKit2 provides highly efficient implementations of several variants of Gillespie's stochastic simulation algorithm (SSA), and tau-leaping with automatic step size selection. StochKit2 features include automatic selection of the optimal SSA method based on model properties, event handling, and automatic parallelism on multicore architectures. The underlying structure of the code has been completely updated to provide a flexible framework for extending its functionality. StochKit2 runs on Linux/Unix, Mac OS X and Windows. It is freely available under GPL version 3 and can be downloaded from http://sourceforge.net/projects/stochkit/. petzold@engineering.ucsb.edu.

  1. The FOT tool kit concept

    NASA Technical Reports Server (NTRS)

    Fatig, Michael

    1993-01-01

    Flight operations and the preparation for it has become increasingly complex as mission complexities increase. Further, the mission model dictates that a significant increase in flight operations activities is upon us. Finally, there is a need for process improvement and economy in the operations arena. It is therefore time that we recognize flight operations as a complex process requiring a defined, structured, and life cycle approach vitally linked to space segment, ground segment, and science operations processes. With this recognition, an FOT Tool Kit consisting of six major components designed to provide tools to guide flight operations activities throughout the mission life cycle was developed. The major components of the FOT Tool Kit and the concepts behind the flight operations life cycle process as developed at NASA's GSFC for GSFC-based missions are addressed. The Tool Kit is therefore intended to increase productivity, quality, cost, and schedule performance of the flight operations tasks through the use of documented, structured methodologies; knowledge of past lessons learned and upcoming new technology; and through reuse and sharing of key products and special application programs made possible through the development of standardized key products and special program directories.

  2. Differential Use of Elementary Science Kits

    NASA Astrophysics Data System (ADS)

    Jones, Gail; Robertson, Laura; Gardner, Grant E.; Dotger, Sharon; Blanchard, Margaret R.

    2012-10-01

    The use of kits in elementary science classes is a growing trend in some countries. Kits provide materials and inquiry lessons in a ready-to-teach format for teachers to use in their science instruction. This study examined elementary teachers' instructional strategies, classroom practices, and assessment types in relation to the frequency of science kit use. A total of 503 elementary teachers from an urban school district received professional development, implemented kits in their classrooms for a year, and then completed a survey about science kit use and teaching practices. Despite similarities in demographic characteristics (gender, ethnicity, certification/educational level), there were significant differences in teachers' use of inquiry-based teaching and assessment practices by kit use. Teachers who reported using kits the most often were significantly more likely to report that their students designed and implemented laboratory investigations as well recorded, represented, and analyzed data. In addition, the high kit users indicated that they were more likely to use student groups, require students to use evidence to support claims, and use alternative assessments of student work including portfolios, notebooks, and long-term projects than those teachers who used kits less frequently. Those teachers who reported using kits the least often were significantly more likely to report having students practice for standardized tests. The role of kits in promoting reform-based teaching practices is discussed.

  3. Genetic interaction between Kit and Scl

    PubMed Central

    Lacombe, Julie; Krosl, Gorazd; Tremblay, Mathieu; Gerby, Bastien; Martin, Richard; Aplan, Peter D.; Lemieux, Sebastien

    2013-01-01

    SCL/TAL1, a tissue-specific transcription factor of the basic helix-loop-helix family, and c-Kit, a tyrosine kinase receptor, control hematopoietic stem cell survival and quiescence. Here we report that SCL levels are limiting for the clonal expansion of Kit+ multipotent and erythroid progenitors. In addition, increased SCL expression specifically enhances the sensitivity of these progenitors to steel factor (KIT ligand) without affecting interleukin-3 response, whereas a DNA-binding mutant antagonizes KIT function and induces apoptosis in progenitors. Furthermore, a twofold increase in SCL levels in mice bearing a hypomorphic Kit allele (W41/41) corrects their hematocrits and deficiencies in erythroid progenitor numbers. At the molecular level, we found that SCL and c-Kit signaling control a common gene expression signature, of which 19 genes are associated with apoptosis. Half of those were decreased in purified megakaryocyte/erythroid progenitors (MEPs) from W41/41 mice and rescued by the SCL transgene. We conclude that Scl operates downstream of Kit to support the survival of MEPs. Finally, higher SCL expression upregulates Kit in normal bone marrow cells and increases chimerism after bone marrow transplantation, indicating that Scl is also upstream of Kit. We conclude that Scl and Kit establish a positive feedback loop in multipotent and MEPs. PMID:23836559

  4. Molecular alterations of KIT oncogene in gliomas.

    PubMed

    Gomes, Ana L; Reis-Filho, Jorge S; Lopes, José M; Martinho, Olga; Lambros, Maryou B K; Martins, Albino; Schmitt, Fernando; Pardal, Fernando; Reis, Rui M

    2007-01-01

    Gliomas are the most common and devastating primary brain tumours. Despite therapeutic advances, the majority of gliomas do not respond either to chemo or radiotherapy. KIT, a class III receptor tyrosine kinase (RTK), is frequently involved in tumourigenic processes. Currently, KIT constitutes an attractive therapeutic target. In the present study we assessed the frequency of KIT overexpression in gliomas and investigated the genetic mechanisms underlying KIT overexpression. KIT (CD117) immunohistochemistry was performed in a series of 179 gliomas of various grades. KIT activating gene mutations (exons 9, 11, 13 and 17) and gene amplification analysis, as defined by chromogenic in situ hybridization (CISH) and quantitative real-time PCR (qRT-PCR) were performed in CD117 positive cases. Tumour cell immunopositivity was detected in 15.6% (28/179) of cases, namely in 25% (1/4) of pilocytic astrocytomas, 25% (5/20) of diffuse astrocytomas, 20% (1/5) of anaplastic astrocytomas, 19.5% (15/77) of glioblastomas and one third (3/9) of anaplastic oligoastrocytomas. Only 5.7% (2/35) of anaplastic oligodendrogliomas showed CD117 immunoreactivity. No association was found between tumour CD117 overexpression and patient survival. In addition, we also observed CD117 overexpression in endothelial cells, which varied from 0-22.2% of cases, being more frequent in high-grade lesions. No KIT activating mutations were identified. Interestingly, CISH and/or qRT-PCR analysis revealed the presence of KIT gene amplification in 6 glioblastomas and 2 anaplastic oligoastrocytomas, corresponding to 33% (8/24) of CD117 positive cases. In conclusion, our results demonstrate that KIT gene amplification rather than gene mutation is a common genetic mechanism underlying KIT expression in subset of malignant gliomas. Further studies are warranted to determine whether glioma patients exhibiting KIT overexpression and KIT gene amplification may benefit from therapy with anti-KIT RTK inhibitors.

  5. Quantifying the transport of subcloud layer reactants by shallow cumulus clouds over the Amazon

    NASA Astrophysics Data System (ADS)

    Ouwersloot, H. G.; Vilà-Guerau de Arellano, J.; van Stratum, B. J. H.; Krol, M. C.; Lelieveld, J.

    2013-12-01

    We investigate the vertical transport of atmospheric chemical reactants from the subcloud layer to the cumulus cloud layer driven by shallow convection over the Amazon during the dry season. The dynamical and chemical assumptions needed for mesoscale and global chemistry transport model parametrizations are systematically analyzed using a Large Eddy Simulation model. We quantify the mass flux transport contribution to the temporal evolution of reactants. Isoprene, a key atmospheric compound over the tropical rain forest, decreases by 8.5% h-1 on average and 15% h-1 at maximum due to mass‒flux‒induced removal. We apply mass flux parametrizations for the transport of chemical reactants and obtain satisfactory agreement with numerically resolved transport, except for some reactants like O3, NO, and NO2. The latter is caused by the local partitioning of reactants, influenced by UV radiation extinction by clouds and small‒scale variability of ambient atmospheric compounds. By considering the longer‒lived NOx (NO + NO2), the transport is well represented by the parametrization. Finally, by considering heterogeneous surface exchange conditions, it is demonstrated that the parametrizations are sensitive to boundary conditions due to changes in the boundary layer dynamics.

  6. Selecting patients for KIT inhibition in melanoma.

    PubMed

    Carvajal, Richard D; Hamid, Omid; Antonescu, Cristina R

    2014-01-01

    For many years, melanoma has been regarded as a single disease in terms of therapeutic considerations. The more recent identification of multiple molecular mechanisms underlying the development, progression, and prognosis of melanoma has led to a new paradigm for the management of this disease, has created new therapeutic opportunities, and has led to improved clinical outcomes. Such advances, however, are dependent upon methods that can reproducibly identify key molecular alterations within an individual tumor, define clinically relevant genetic subgroups of disease, and permit improved patient selection for targeted therapies.Melanomas harboring genetic alterations of KIT have been demonstrated to constitute one such molecular subgroup of disease. In this chapter, we will discuss the biology of KIT in melanoma, review the rationale for and clinical data regarding KIT inhibition in melanomas harboring activating alterations of KIT, propose guidelines for the selection of patients for KIT inhibitor therapy, and, finally, present laboratory methods for KIT assessment in melanoma.

  7. Aflatoxin plate kit. Performance Tested Method 081003.

    PubMed

    Trombley, Arthur; Fan, Titan; LaBudde, Robert

    2011-01-01

    The level of total aflatoxin contamination was analyzed in naturally contaminated and spiked samples of corn and peanut using the Aflatoxin Plate Kit. This kit is an enzyme-linked immunosorbent assay (ELISA) suitable for rapid testing of grains and peanuts. The assay was evaluated for ruggedness and linearity of the standard curve. The test kit results were then statistically evaluated for accuracy, precision, and correlation to a validated HPLC method (AOAC 994.08). The results were verified by an independent laboratory.

  8. Prehospital Cricothyrotomy Kits Used in Combat.

    PubMed

    Schauer, Steven G; April, Michael D; Cunningham, Cord W; Long, Adrianna N; Carter, Robert

    2017-01-01

    Surgical cricothyrotomy remains the only definitive airway management modality for the tactical setting recommended by Tactical Combat Casualty Care guidelines. Some units have fielded commercial cricothyrotomy kits to assist Combat Medics with surgical cricothyrotomy. To our knowledge, no previous publications report data on the use of these kits in combat settings. This series reports the the use of two kits in four patients in the prehospital combat setting. Using the Department of Defense Trauma Registry and the Prehospital Trauma Registry, we identified four cases of patients who underwent prehospital cricothyrotomy with the use of commercial kits. In the first two cases, a Medic successfully used a North American Rescue CricKit (NARCK) to obtain a surgical airway in a Servicemember with multiple amputations from an improvised explosive device explosion. In case 3, the Medic unsuccessfully used an H&H Medical kit to attempt placement of a surgical airway in a Servicemember shot in the head by small arms fire. A second attempt to place a surgical airway using a NARCK was successful. In case 4, a Soldier sustained a gunshot wound to the chest. A Medic described fluid in the airway precluding bag-valve-mask ventilation; the Medic attempted to place a surgical airway with the H&H kit without success. Four cases of prehospital surgical airway cannulation on the battlefield demonstrated three successful uses of prehospital cricothyrotomy kits. Further research should focus on determining which kits may be most useful in the combat setting. 2017.

  9. Genetic interaction between Kit and Scl.

    PubMed

    Lacombe, Julie; Krosl, Gorazd; Tremblay, Mathieu; Gerby, Bastien; Martin, Richard; Aplan, Peter D; Lemieux, Sebastien; Hoang, Trang

    2013-08-15

    SCL/TAL1, a tissue-specific transcription factor of the basic helix-loop-helix family, and c-Kit, a tyrosine kinase receptor, control hematopoietic stem cell survival and quiescence. Here we report that SCL levels are limiting for the clonal expansion of Kit⁺ multipotent and erythroid progenitors. In addition, increased SCL expression specifically enhances the sensitivity of these progenitors to steel factor (KIT ligand) without affecting interleukin-3 response, whereas a DNA-binding mutant antagonizes KIT function and induces apoptosis in progenitors. Furthermore, a twofold increase in SCL levels in mice bearing a hypomorphic Kit allele (W41/41) corrects their hematocrits and deficiencies in erythroid progenitor numbers. At the molecular level, we found that SCL and c-Kit signaling control a common gene expression signature, of which 19 genes are associated with apoptosis. Half of those were decreased in purified megakaryocyte/erythroid progenitors (MEPs) from W41/41 mice and rescued by the SCL transgene. We conclude that Scl operates downstream of Kit to support the survival of MEPs. Finally, higher SCL expression upregulates Kit in normal bone marrow cells and increases chimerism after bone marrow transplantation, indicating that Scl is also upstream of Kit. We conclude that Scl and Kit establish a positive feedback loop in multipotent and MEPs.

  10. Control of the reactant ion chemistry for the analysis of explosives by ion mobility spectroscopy

    SciTech Connect

    Rodacy, P.J.; Ingersoll, D.

    1995-07-01

    Changes in the reactant ion composition in the ion mobility spectrometer (IMS) can result in a change in the ionization processes occurring in the ionization region, ultimately leading to an altered instrumental response for the analyte, and exacerbating the problem of qualitative and quantitative analysis. Some species are very susceptible to changes in reactant ions, while other species are relatively unaffected. These types of behavior are observed for two common explosives, namely, hexahydro-1,3,5-trinitrol,3,5-triazine (RDX) and 1,3,5-trinitrotoluene (TNT), respectively. To control the reactant ion composition, and hence the gas phase chemistry, it is necessary to control the composition of gases present in the ionization region of the IMS. A series of modifications are described for the PCP Phemto-Chem 100 IMS that afford the requisite control. The effectiveness of these modifications for analysis of RDX and TNT are described and contrasted with that observed for the unmodified system.

  11. Fuel cell systems for First Lunar Outpost-reactant storage option

    NASA Technical Reports Server (NTRS)

    Nelson, P. A.

    1995-01-01

    The office of Space, DOE, appointed a Lunar Surface Power Working Group to review candidate systems for the First Lunar Outpost habitat. The working group met for a total of five days in the fall of 1992 and concluded that the candidate involving a photovoltaic unit, a fuel cell, a regenerator to recycle the reactants, and storage of oxygen and hydrogen gases was the most attractive for this application. Most of the volume (97 percent) and weight (63 percent) are taken up by the reactants and their storage tanks. Therefore, in my work for the Group, and in this report, I have concentrated on finding ways to reduce these volumes and weights. Three options were considered: (1) the baseline case considered in the preliminary system design, that of separate high pressure (200 bar) storage tanks; (2) the use of two of the descent storage propellant tanks wrapped with graphite fibers to increase the pressure capability; and (3) the use of cryogenic storage of reactants in the propellant tanks. The first option results in high storage tank mass and volume. The second option saves 90 percent of the volume by making use of the propellant tanks, but it has little if any weight advantages; the weight saved by not providing extra tanks for reactant storage is nearly entirely added back by the weight of the additional material (graphite fibers) to strengthen the propellant tanks. Use of the descent storage propellant tanks for storage of the fuel cell reactants as cryogenic liquids requires a gas liquefaction system. The weight of this system is expected to be less than that of the storage tanks but it would require development and testing to prove its reliability. The solar array would have to be 40 percent larger and the heat projection range would be 170 percent larger than for storage of reactants as high pressure gases. For a high power system (greater than 20 kW) the larger energy storage requirement would probably favor the cryogenic storage option.

  12. Enhanced bactericidal action of acidified sodium chlorite caused by the saturation of reactants.

    PubMed

    Kim, N H; Park, T H; Rhee, M S

    2014-06-01

    Factors affecting the antibacterial action of acidified sodium chlorite (ASC), a widely used disinfectant, have not been determined. This study investigated the significant factors suggesting efficient production method to maximize bactericidal action of ASC. The effects of (i) preparation procedures (total three methods); (ii) initial concentrations of reactants: sodium chlorite (SC) and citric acid (CTA) (up to maximum solubility of each reactant) and (iii) final pH values (3·0 and 2·5) to the bactericidal action of ASC were investigated with a fixed final concentration of SC (10 ppm) using various foodborne pathogens (Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium and Staphylococcus aureus). The antimicrobial compounds produced and the bactericidal effects depended on the preparation procedure and the initial concentrations of the reactants. The ASC prepared by premixing highly concentrated reactants (in particular > 40%) followed by dilution (dilution after reaction, DAR) was more effective in inactivating foodborne pathogens, and it produced higher antimicrobial compound (Cl(2) and ClO(2)) yields than the other procedures. A 5-min treatment with ASC, produced using the other procedures, resulted in a reduction of < 3·5 log CFU ml(-1) (Gram positive = 0·18-0·78; Gram negative = 0·03-3·49 log CFU ml(-1)), whereas ASC produced with the DAR procedure using the saturated reactants completely inactivated all of the test pathogens within 5 min without recovery (initial concentration = 6·94-7·08 log CFU ml(-1)). The ASC production with the DAR procedure using the saturated reactants maximizes both the antimicrobial compound yields and bactericidal effects of the ASC solutions. This study will contribute to increase the efficiency of ASC treatments for disinfections reducing the effective SC concentrations for industrial use. © 2014 The Society for Applied Microbiology.

  13. Reaction front dynamics of A + B --> C with initially separated reactants

    NASA Astrophysics Data System (ADS)

    Araujo, Mariela; Larralde, Hernan; Havlin, Shlomo; Stanely, H. E.

    1992-12-01

    We study the reaction front for the diffusion-reaction system A + B → C with initially separated reactants. We present analytical results for the form of the front and the exponents that characterize its width and height asymptotically, which appear to hold in d ⩾ 2. We also present analytical and numerical results for the form of the reaction front for the 1D case in which one of the reactants is static, and find the width exponent α = {1}/{4}, which is larger than the mean field exponent (α = {1}/{6}) but smaller than the value for the case in which both species diffuse (α ⋍ 0.3).

  14. Role of inlet reactant mixedness on the thermodynamic performance of a rotating detonation engine

    NASA Astrophysics Data System (ADS)

    Nordeen, C. A.; Schwer, D.; Schauer, F.; Hoke, J.; Barber, T.; Cetegen, B. M.

    2016-07-01

    Rotating detonation engines have the potential to achieve the high propulsive efficiencies of detonation cycles in a simple and effective annular geometry. A two-dimensional Euler simulation is modified to include mixing factors to simulate the imperfect mixing of injected reactant streams. Contrary to expectations, mixing is shown to have a minimal impact on performance. Oblique detonation waves are shown to increase local stream thermal efficiency, which compensates for other losses in the flow stream. The degree of reactant mixing is, however, a factor in controlling the stability and existence of rotating detonations.

  15. Reactant conversion in homogeneous turbulence - Mathematical modeling, computational validations, and practical applications

    NASA Technical Reports Server (NTRS)

    Madnia, C. K.; Frankel, S. H.; Givi, P.

    1992-01-01

    The presently obtained closed-form analytical expressions, which predict the limiting rate of mean reactant conversion in homogeneous turbulent flows under the influence of a binary reaction, are derived via the single-point pdf method based on amplitude mapping closure. With this model, the maximum rate of the mean reactant's decay can be conveniently expressed in terms of definite integrals of the parabolic cylinder functions. The results obtained are shown to be in good agreement with data generated by direct numerical simulations.

  16. Theoretical treatment of reversible energy transfer reactions of metastable reactants: Modification of the integral encounter theory

    NASA Astrophysics Data System (ADS)

    Ivanov, Konstantin L.

    2005-08-01

    Corrections to the results of the integral encounter theory (IET) of reversible energy transfer reaction provided by two competing approaches of the modified encounter theory (MET) and the many-particle kernel-theory (MPK-theory) are considered in detail and compared with each other. The present treatment establishes that the MET is more preferable for improving the IET at higher reactant densities. Predictions of the MET and MPK-theory for the quantum yields of luminescence and quenching constants are compared with each other, reversible transfer kinetics from metastable reactant is studied in the framework of the MET.

  17. Direct qPCR quantification using the Quantifiler(®) Trio DNA quantification kit.

    PubMed

    Liu, Jason Yingjie

    2014-11-01

    The effectiveness of a direct quantification assay is essential to the adoption of the combined direct quantification/direct STR workflow. In this paper, the feasibility of using the Quantifiler(®) Trio DNA quantification kit for the direct quantification of forensic casework samples was investigated. Both low-level touch DNA samples and blood samples were collected on PE swabs and quantified directly. The increased sensitivity of the Quantifiler(®) Trio kit enabled the detection of less than 10pg of DNA in unprocessed touch samples and also minimizes the stochastic effect experienced by different targets in the same sample. The DNA quantity information obtained from a direct quantification assay using the Quantifiler(®) Trio kit can also be used to accurately estimate the optimal input DNA quantity for a direct STR amplification reaction. The correlation between the direct quantification results (Quantifiler(®) Trio kit) and the direct STR results (GlobalFiler™ PCR amplification kit(*)) for low-level touch DNA samples indicates that direct quantification using the Quantifiler(®) Trio DNA quantification kit is more reliable than the Quantifiler(®) Duo DNA quantification kit for predicting the STR results of unprocessed touch DNA samples containing less than 10pg of DNA.

  18. The Project Manager's Tool Kit

    NASA Technical Reports Server (NTRS)

    Cameron, W. Scott

    2003-01-01

    Project managers are rarely described as being funny. Moreover, a good sense of humor rarely seems to be one of the deciding factors in choosing someone to be a project manager, or something that pops up as a major discussion point at an annual performance review. Perhaps this is because people think you aren't serious about your work if you laugh. I disagree with this assessment, but that's not really my point. As I talk to people either pursuing a career in project management, or broadening their assignment to include project management, I encourage them to consider what tools they need to be successful. I suggest that they consider any strength they have to be part of their Project Management (PM) Tool Kit, and being funny could be one of the tools they need.

  19. Building Use Policies. SPEC Kit 144.

    ERIC Educational Resources Information Center

    Coyle, Patrick

    This Systems and Procedures Exchange Center (SPEC) flyer/kit addresses some of the needs of Association of Research Libraries (ARL) members regarding library building use and the aesthetics and well-being of the materials and people in the building. The kit draws upon documents gathered as part of a 1986 Quick-SPEC survey that dealt with food and…

  20. Special tool kit aids heavily garmented workers

    NASA Technical Reports Server (NTRS)

    Holmes, A. E.

    1966-01-01

    Triangular aluminum tool kit, filled with polyurethane is constructed to receive various tools and hold them in a snug but quick-release fit as an aid to heavily gloved workers. The kit is designed to allow mounting within easily accessable reach and to provide protection of the tools during storage.

  1. Anatomy of a Minicourse. Audiovisual Kit.

    ERIC Educational Resources Information Center

    Meyer, Rex

    This kit explains the use of minicourses at Macquarie University in New South Wales, Australia, which were designed for the inservice training of teachers. The kit contains the script of the audio commentary, a cassette tape, color slides, and a booklet entitled "Anatomy of the Minicourse." The tape briefly describes the minicourses in general and…

  2. CARE Kit: Combining Activities with Real Experiences.

    ERIC Educational Resources Information Center

    Eastern Illinois Univ., Charleston.

    Materials presented in this kit form a package containing eighty-six vocational development oriented activities designed primarily for individuals and small groups of underachieving junior high or high school students and is intended to supplement regular classroom activities. Included in the kit are the following: (1) a teacher's guide containing…

  3. Habitats NatureScope[R] Kit.

    ERIC Educational Resources Information Center

    Emerson, Eva

    This kit introduces the basic concepts of habitat through challenging and engaging interdisciplinary, hands-on activities. The material is designed for K-8 educators working in traditional and non- traditional classrooms with the goals of increasing awareness of the ways habitats work and why they are important. This kit consists of four…

  4. Kid's PACK: Population Awareness Campaign Kit.

    ERIC Educational Resources Information Center

    Zero Population Growth, Inc., Washington, DC.

    This fun and educational kit is designed specifically for elementary students. The "Kid's PACK" (Population Awareness Campaign Kit) entertains and informs children on the environment and human population growth through stories, games, and concrete ideas for making a difference. In three booklets, the "Kid's PACK" offers…

  5. Blood collection kit for Space Lab 1

    NASA Image and Video Library

    1981-02-02

    S81-26158 (Feb 1981) --- A close-up view of a training version of a STS-40/SLS-1 blood kit. Blood samples from crewmembers are critical to a number of Space Life Sciences-1 (SLS-1) investigations. One day's collection equipment, color coded for each crewmember, is neatly organized in the kit.

  6. Features of the Java commodity grid kit.

    SciTech Connect

    von Laszewski, G.; Gawor, J.; Lane, P.; Rehn, N.; Russell, M.; Mathematics and Computer Science

    2002-11-01

    In this paper we report on the features of the Java Commodity Grid Kit (Java CoG Kit). The Java CoG Kit provides middleware for accessing Grid functionality from the Java framework. Java CoG Kit middleware is general enough to design a variety of advanced Grid applications with quite different user requirements. Access to the Grid is established via Globus Toolkit protocols, allowing the Java CoG Kit to also communicate with the services distributed as part of the C Globus Toolkit reference implementation. Thus, the Java CoG Kit provides Grid developers with the ability to utilize the Grid, as well as numerous additional libraries and frameworks developed by the Java community to enable network, Internet, enterprise and peer-to-peer computing. A variety of projects have successfully used the client libraries of the Java CoG Kit to access Grids driven by the C Globus Toolkit software. In this paper we also report on the efforts to develop serverside Java CoG Kit components. As part of this research we have implemented a prototype pure Java resource management system that enables one to run Grid jobs on platforms on which a Java virtual machine is supported, including Windows NT machines.

  7. A Prototype Grammar Kit in Prolog.

    ERIC Educational Resources Information Center

    Kahn, Kenneth M.

    1984-01-01

    Presents a prototype of a computerized grammar kit written in PROLOG and designed for children interested in exploring language. PROLOG's advantages for building parsers, generators, translators, and question-answering systems are discussed, and a scenario of a child working on a grammar project using the kit and implementation issues are…

  8. Affirmative Action Programs. SPEC Kit 67.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This Systems and Procedures Exchange Center (SPEC) kit on affirmative action programs, which replaces an earlier SPEC kit on affirmative action, contains planning documents from Association of Research Libraries (ARL) member institutions and policy statements on affirmative action and equal employment opportunity from several universities. The kit…

  9. Culture Kits for the Elementary Classroom.

    ERIC Educational Resources Information Center

    Hickey, M. Gail

    1997-01-01

    Outlines an instructional unit where students construct culture kits illustrating a specific culture. Culture kits are constructed out of realia and other material including maps, travel brochures, photographs, newspapers, souvenirs, and other items. Discusses collecting these items and possible multicultural applications. (MJP)

  10. Chronocoulometry: A Convenient, Rapid and Reliable Technique for Detection and Determination of Adsorbed Reactants.

    ERIC Educational Resources Information Center

    Anson, Fred C.; Osteryoung, Robert A.

    1983-01-01

    Measurement of the time dependence of the flow of charge constitutes the procedure known as chronocoulometry. Although the technique is useful in a variety of electrochemical measurements, the discussion focuses on the evaluation of reactant adsorption, the application for which the technique was originally devised. (JN)

  11. Teachers' Misconceptions about the Effects of Addition of More Reactants or Products on Chemical Equilibrium

    ERIC Educational Resources Information Center

    Cheung, Derek; Ma, Hong-jia; Yang, Jie

    2009-01-01

    The importance of research on misconceptions about chemical equilibrium is well recognized by educators, but in the past, researchers' interest has centered on student misconceptions and has neglected teacher misconceptions. Focusing on the effects of adding more reactants or products on chemical equilibrium, this article discusses the various…

  12. Solid oxide fuel cell systems with hot zones having improved reactant distribution

    DOEpatents

    Poshusta, Joseph C.; Booten, Charles W.; Martin, Jerry L.

    2012-11-06

    A Solid Oxide Fuel Cell (SOFC) system having a hot zone with a center cathode air feed tube for improved reactant distribution, a CPOX reactor attached at the anode feed end of the hot zone with a tail gas combustor at the opposing end for more uniform heat distribution, and a counter-flow heat exchanger for efficient heat retention.

  13. Solid oxide fuel cell systems with hot zones having improved reactant distribution

    DOEpatents

    Poshusta, Joseph C; Booten, Charles W; Martin, Jerry L

    2013-12-24

    A Solid Oxide Fuel Cell (SOFC) system having a hot zone with a center cathode air feed tube for improved reactant distribution, a CPOX reactor attached at the anode feed end of the hot zone with a tail gas combustor at the opposing end for more uniform heat distribution, and a counter-flow heat exchanger for efficient heat retention.

  14. Solid oxide fuel cell systems with hot zones having improved reactant distribution

    DOEpatents

    Poshusta, Joseph C.; Booten, Charles W.; Martin, Jerry L.

    2016-05-17

    A Solid Oxide Fuel Cell (SOFC) system having a hot zone with a center cathode air feed tube for improved reactant distribution, a CPOX reactor attached at the anode feed end of the hot zone with a tail gas combustor at the opposing end for more uniform heat distribution, and a counter-flow heat exchanger for efficient heat retention.

  15. Teachers' Misconceptions about the Effects of Addition of More Reactants or Products on Chemical Equilibrium

    ERIC Educational Resources Information Center

    Cheung, Derek; Ma, Hong-jia; Yang, Jie

    2009-01-01

    The importance of research on misconceptions about chemical equilibrium is well recognized by educators, but in the past, researchers' interest has centered on student misconceptions and has neglected teacher misconceptions. Focusing on the effects of adding more reactants or products on chemical equilibrium, this article discusses the various…

  16. Reduction of titanium dioxide to metallic titanium conducted under the autogenic pressure of the reactants.

    PubMed

    Eshed, Michal; Irzh, Alexander; Gedanken, Aharon

    2009-08-03

    We report on a reaction to convert titanium dioxide to titanium. The reduction reaction was done under the autogenic pressure of the reactants at 750 degrees C for 5 h. The MgO, a by-product, was removed by acids to obtain pure metallic titanium.

  17. Isolation of a lipopolysaccharide-binding acute phase reactant from rabbit serum.

    PubMed

    Tobias, P S; Soldau, K; Ulevitch, R J

    1986-09-01

    This report describes the purification of an acute phase reactant from acute phase rabbit serum, which endows normal serum with the properties of acute phase serum, insofar as LPS is concerned. The acute phase reactant is referred to as LPS-binding protein, or LBP. LBP was purified approximately 2,000-fold by chromatography of acute phase serum on Bio-Rex 70 and Mono-Q resins. The resulting preparation consisted of two glycoproteins having molecular weights of 60,500 and 58,000; the two were obtained in a variable ratio, usually near 10:1, respectively. After separation by SDS-PAGE, the N-terminal 36 amino acid sequences of the two proteins were identical. From the N-terminal sequence, as well as other properties of LBP, LBP appears to be unrelated to any known acute phase reactants. The direct interaction of LPS and LBP was inferred from two types of evidence: first, immunoprecipitation of [3H]LPS from APRS by anti-LBP sera; and second, by the 125I-labeling of LBP when APRS-containing 125I-labeled 2-(p-azidosalicylamido)ethyl 1,3'-dithiopropionyl-LPS was photolysed. The data presented here support the concept that the 60-kD glycoprotein we have termed LBP is a newly recognized acute phase reactant that may modulate the biochemical and biologic properties of LPS in vivo.

  18. Genericity of confined chemical garden patterns with regard to changes in the reactants.

    PubMed

    Haudin, Florence; Brasiliense, V; Cartwright, Julyan H E; Brau, Fabian; De Wit, A

    2015-05-21

    The growth of chemical gardens is studied experimentally in a horizontal confined geometry when a solution of metallic salt is injected into an alkaline solution at a fixed flow rate. Various precipitate patterns are observed-spirals, flowers, worms or filaments-depending on the reactant concentrations. In order to determine the relative importance of the chemical nature of the reactants and physical processes in the pattern selection, we compare the structures obtained by performing the same experiment using different pairs of reactants of varying concentrations with cations of calcium, cobalt, copper, and nickel, and anions of silicate and carbonate. We show that although the transition zones between different patterns are not sharply defined, the morphological phase diagrams are similar in the various cases. We deduce that the nature of the chemical reactants is not a key factor for the pattern selection in the confined chemical gardens studied here and that the observed morphologies are generic patterns for precipitates possessing a given level of cohesiveness when grown under certain flow conditions.

  19. Chronocoulometry: A Convenient, Rapid and Reliable Technique for Detection and Determination of Adsorbed Reactants.

    ERIC Educational Resources Information Center

    Anson, Fred C.; Osteryoung, Robert A.

    1983-01-01

    Measurement of the time dependence of the flow of charge constitutes the procedure known as chronocoulometry. Although the technique is useful in a variety of electrochemical measurements, the discussion focuses on the evaluation of reactant adsorption, the application for which the technique was originally devised. (JN)

  20. Evaluation of RealStar Reverse Transcription–Polymerase Chain Reaction Kits for Filovirus Detection in the Laboratory and Field

    PubMed Central

    Rieger, Toni; Kerber, Romy; El Halas, Hussein; Pallasch, Elisa; Duraffour, Sophie; Günther, Stephan; Ölschläger, Stephan

    2016-01-01

    Background. Diagnosis of Ebola virus (EBOV) disease (EVD) requires laboratory testing. Methods. The RealStar Filovirus Screen reverse transcription–polymerase chain reaction (RT-PCR) kit and the derived RealStar Zaire Ebolavirus RT-PCR kit were validated using in vitro transcripts, supernatant of infected cell cultures, and clinical specimens from patients with EVD. Results. The Filovirus Screen kit detected EBOV, Sudan virus, Taï Forest virus, Bundibugyo virus, Reston virus, and Marburg virus and differentiated between the genera Ebolavirus and Marburgvirus. The amount of filovirus RNA that could be detected with a probability of 95% ranged from 11 to 67 RNA copies/reaction on a LightCycler 480 II. The Zaire Ebolavirus kit is based on the Filovirus Screen kit but was optimized for detection of EBOV. It has an improved signal-to-noise ratio at low EBOV RNA concentrations and is somewhat more sensitive than the Filovirus kit. Both kits show significantly lower analytical sensitivity on a SmartCycler II. Clinical evaluation revealed that the SmartCycler II, compared with other real-time PCR platforms, decreases the clinical sensitivity of the Filovirus Screen kit to diagnose EVD at an early stage. Conclusions. The Filovirus Screen kit detects all human-pathogenic filoviruses with good analytical sensitivity if performed on an appropriate real-time PCR platform. High analytical sensitivity is important for early diagnosis of EVD. PMID:27549586

  1. Novel Adsorbent-Reactants for Treatment of Ash and Scrubber Pond Effluents

    SciTech Connect

    Bill Batchelor; Dong Suk Han; Eun Jung Kim

    2010-01-31

    The overall goal of this project was to evaluate the ability of novel adsorbent/reactants to remove specific toxic target chemicals from ash and scrubber pond effluents while producing stable residuals for ultimate disposal. The target chemicals studied were arsenic (As(III) and As(V)), mercury (Hg(II)) and selenium (Se(IV) and Se(VI)). The adsorbent/reactants that were evaluated are iron sulfide (FeS) and pyrite (FeS{sub 2}). Procedures for measuring concentrations of target compounds and characterizing the surfaces of adsorbent-reactants were developed. Effects of contact time, pH (7, 8, 9, 10) and sulfate concentration (0, 1, 10 mM) on removal of all target compounds on both adsorbent-reactants were determined. Stability tests were conducted to evaluate the extent to which target compounds were released from the adsorbent-reactants when pH changed. Surface characterization was conducted with x-ray photoelectron spectroscopy (XPS) to identify reactions occurring on the surface between the target compounds and surface iron and sulfur. Results indicated that target compounds could be removed by FeS{sub 2} and FeS and that removal was affected by time, pH and surface reactions. Stability of residuals was generally good and appeared to be affected by the extent of surface reactions. Synthesized pyrite and mackinawite appear to have the required characteristics for removing the target compounds from wastewaters from ash ponds and scrubber ponds and producing stable residuals.

  2. 100G Deployment@(DE-KIT)

    NASA Astrophysics Data System (ADS)

    Hoeft, Bruno; Petzold, Andreas

    2015-12-01

    The Steinbuch Centre for Computing (SCC) at Karlsruhe Institute of Technology (KIT) has been involved fairly early in 100GE network technology. Initiated by DFN1 (the German NREN), a first 100GE wide area network testbed over a distance of approx. 450 km was deployed between the national research organizations KIT and FZ-Jülich in 2010. Three years later in 2013. KIT joined the Caltech SuperComputing 2013 (SC132) 100GE "show floor" initiative using the transatlantic ANA-100GE link to transfer LHC data from a storage at DE-KIT (GridKa) in Europe to hard disks at the show floor of SC13 in Denver (USA). The network infrastructure of KIT as well as of the German Tier-1 installation DE-KIT (GridKa). however. is still based on 10Gbps. As highlighted in the contribution "Status and Trends in Networking at LHC Tier1 Facilities" to CHEP 2012. proactive investment is required at the Tier-1 sites. Bandwidth requirements will grow beyond current capacity and the required upgrades are expected in 2015. In close cooperation with DFN. KIT drives the upgrade from 10GE to 100GE. The process is divided into several phases. due to upgrade costs and differing requirements in different parts of the network infrastructure. The requirements of the different phases as well as the planned topology will be described. Some of the obstacles we discovered during the deployment will be discussed and solutions or workarounds presented.

  3. Optimism

    PubMed Central

    Carver, Charles S.; Scheier, Michael F.; Segerstrom, Suzanne C.

    2010-01-01

    Optimism is an individual difference variable that reflects the extent to which people hold generalized favorable expectancies for their future. Higher levels of optimism have been related prospectively to better subjective well-being in times of adversity or difficulty (i.e., controlling for previous well-being). Consistent with such findings, optimism has been linked to higher levels of engagement coping and lower levels of avoidance, or disengagement, coping. There is evidence that optimism is associated with taking proactive steps to protect one's health, whereas pessimism is associated with health-damaging behaviors. Consistent with such findings, optimism is also related to indicators of better physical health. The energetic, task-focused approach that optimists take to goals also relates to benefits in the socioeconomic world. Some evidence suggests that optimism relates to more persistence in educational efforts and to higher later income. Optimists also appear to fare better than pessimists in relationships. Although there are instances in which optimism fails to convey an advantage, and instances in which it may convey a disadvantage, those instances are relatively rare. In sum, the behavioral patterns of optimists appear to provide models of living for others to learn from. PMID:20170998

  4. The SCRAM tool-kit

    NASA Technical Reports Server (NTRS)

    Tamir, David; Flanigan, Lee A.; Weeks, Jack L.; Siewert, Thomas A.; Kimbrough, Andrew G.; Mcclure, Sidney R.

    1994-01-01

    This paper proposes a new series of on-orbit capabilities to support the near-term Hubble Space Telescope, Extended Duration Orbiter, Long Duration Orbiter, Space Station Freedom, other orbital platforms, and even the future manned Lunar/Mars missions. These proposed capabilities form a toolkit termed Space Construction, Repair, and Maintenance (SCRAM). SCRAM addresses both intra-Vehicular Activity (IVA) and Extra-Vehicular Activity (EVA) needs. SCRAM provides a variety of tools which enable welding, brazing, cutting, coating, heating, and cleaning, as well as corresponding nondestructive examination. Near-term IVA-SCRAM applications include repair and modification to fluid lines, structure, and laboratory equipment inside a shirt-sleeve environment (i.e. inside Spacelab or Space Station). Near-term EVA-SCRAM applications include construction of fluid lines and structural members, repair of punctures by orbital debris, refurbishment of surfaces eroded by contaminants. The SCRAM tool-kit also promises future EVA applications involving mass production tasks automated by robotics and artificial intelligence, for construction of large truss, aerobrake, and nuclear reactor shadow shields structures. The leading candidate tool processes for SCRAM, currently undergoing research and development, include Electron Beam, Gas Tungsten Arc, Plasma Arc, and Laser Beam. A series of strategic space flight experiments would make SCRAM available to help conquer the space frontier.

  5. Telescience Resource Kit Software Lifecycle

    NASA Technical Reports Server (NTRS)

    Griner, Carolyn S.; Schneider, Michelle

    1998-01-01

    The challenge of a global operations capability led to the Telescience Resource Kit (TReK) project, an in-house software development project of the Mission Operations Laboratory (MOL) at NASA's Marshall Space Flight Center (MSFC). The TReK system is being developed as an inexpensive comprehensive personal computer- (PC-) based ground support system that can be used by payload users from their home sites to interact with their payloads on board the International Space Station (ISS). The TReK project is currently using a combination of the spiral lifecycle model and the incremental lifecycle model. As with any software development project, there are four activities that can be very time consuming: Software design and development, project documentation, testing, and umbrella activities, such as quality assurance and configuration management. In order to produce a quality product, it is critical that each of these activities receive the appropriate amount of attention. For TReK, the challenge was to lay out a lifecycle and project plan that provides full support for these activities, is flexible, provides a way to deal with changing risks, can accommodate unknowns, and can respond to changes in the environment quickly. This paper will provide an overview of the TReK lifecycle, a description of the project's environment, and a general overview of project activities.

  6. On the mechanism of effective chemical reactions with turbulent mixing of reactants and finite rate of molecular reactions

    NASA Astrophysics Data System (ADS)

    Vorotilin, V. P.

    2017-01-01

    A generalization of the theory of chemical transformation processes under turbulent mixing of reactants and arbitrary values of the rate of molecular reactions is presented that was previously developed for the variant of an instantaneous reaction [13]. The use of the features of instantaneous reactions when considering the general case, namely, the introduction of the concept of effective reaction for the reactant volumes and writing a closing conservation equation for these volumes, became possible due to the partition of the whole amount of reactants into "active" and "passive" classes; the reactants of the first class are not mixed and react by the mechanism of instantaneous reactions, while the reactants of the second class approach each other only through molecular diffusion, and therefore their contribution to the reaction process can be neglected. The physical mechanism of reaction for the limit regime of an ideal mixing reactor (IMR) is revealed and described. Although formally the reaction rate in this regime depends on the concentration of passive fractions of the reactants, according to the theory presented, the true (hidden) mechanism of the reaction is associated only with the reaction of the active fractions of the reactants with vanishingly small concentration in the volume of the reactor. It is shown that the rate constant of fast chemical reactions can be evaluated when the mixing intensity of reactants is much less than that needed to reach the mixing conditions in an IMR.

  7. Analysis of KIT expression and KIT exon 11 mutations in canine oral malignant melanomas.

    PubMed

    Murakami, A; Mori, T; Sakai, H; Murakami, M; Yanai, T; Hoshino, Y; Maruo, K

    2011-09-01

    KIT, a transmembrane receptor tyrosine kinase, is one of the specific targets for anti-cancer therapy. In humans, its expression and mutations have been identified in malignant melanomas and therapies using molecular-targeted agents have been promising in these tumours. As human malignant melanoma, canine malignant melanoma is a fatal disease with metastases and the poor response has been observed with all standard protocols. In our study, KIT expression and exon 11 mutations in dogs with histologically confirmed malignant oral melanomas were evaluated. Although 20 of 39 cases were positive for KIT protein, there was no significant difference between KIT expression and overall survival. Moreover, polymerase chain reaction amplification and sequencing of KIT exon 11 in 17 samples did not detect any mutations and proved disappointing. For several reasons, however, KIT expression and mutations of various exons including exon 11 should be investigated in more cases.

  8. Ziff-Gulari-Barshad model with CO desorption under oscillating reactant pressure

    NASA Astrophysics Data System (ADS)

    Sinha, I.; Mukherjee, A. K.

    2010-08-01

    We study the Ziff-Gulari-Barshad (ZGB) model subjected to periodic variation of reactant pressure, without desorption as well as at different desorption rates. Keeping the time period of oscillation fixed at a small value, Monte Carlo simulations are carried out at different amplitudes. With increase in the amplitude of oscillation, a transition occurs from the reactive state to the (partially) poisoned state. The reactant pressure oscillation is periodic cosine and symmetric about the ZGB discontinuous phase transition. Even for the model with no desorption, the numerical data for the fourth-order cumulant and the finite size scaling analysis suggest that the system does not undergo any phase transition but only a smooth crossover from the reactive to the poisoned state.

  9. The sensitized photocatalysis of a mixed reactant system of a 4-chlorophenol and 4-nitrophenol

    SciTech Connect

    Dieckmann, M.S.; Gray, K.A.; Kamat, P.V.

    1994-12-31

    Sensitized photocatalysis has been demonstrated with colored pollutants such as nitrophenols. With visible light excitation of an adsorbed nitrophenolic compound, charge injection into the conduction band of the semiconductor occurs and subsequent transformation of nitrophenols to a variety of products is possible. These phenomena have been observed in powder systems of TiO{sub 2} but have not been clearly detailed in aqueous systems. This work reports the results of aqueous TiO{sub 2} slurry experiments in which the degradation of 4-nitrophenol and 4-chlorophenol is studied in single reactant and mixed reactant systems. The primary objectives of this work are to establish the reaction pathway occurring in sensitized photocatalysis and to determine if sensitization can induce/drive the degradation of non-colored pollutants. The influence of organic concentration, oxygen and light energy are discussed and byproduct data are presented.

  10. Reaction front for A+B-->C diffusion-reaction systems with initially separated reactants

    NASA Astrophysics Data System (ADS)

    Larralde, Hernan; Araujo, Mariela; Havlin, Shlomo; Stanley, H. Eugene

    1992-07-01

    We study both analytically and numerically the A+B-->C reaction-diffusion system for the case of initially separated reactants. We find the functional forms of the reaction front and also of the concentration profiles of the reactants. The profile of the production rate R(x,t) at site x and time t scales as t-2/3(x/t1/6)3/4exp[-2/3 (λx/t1/6)3/2] for the spatial range t1/6<

  11. Acute-phase reactants in periodontal disease: current concepts and future implications.

    PubMed

    Archana, Vilasan; Ambili, Ranjith; Nisha, Krishnavilasam Jayakumary; Seba, Abraham; Preeja, Chandran

    2015-05-01

    Periodontal disease has been linked to adverse cardiovascular events by unknown mechanisms. C-reactive protein is a systemic marker released during the acute phase of an inflammatory response and is a prognostic marker for cardiovascular disease, with elevated serum levels being reported during periodontal disease. Studies also reported elevated levels of various other acute-phase reactants in periodontal disease. It has been reported extensively in the literature that treatment of periodontal infections can significantly lower serum levels of C-reactive protein. Therefore, an understanding of the relationship between acute-phase response and the progression of periodontal disease and other systemic health complications would have a profound effect on the periodontal treatment strategies. In view of this fact, the present review highlights an overview of acute-phase reactants and their role in periodontal disease. © 2014 Wiley Publishing Asia Pty Ltd.

  12. Analysis of the H + D2 reaction mechanism through consideration of the intrinsic reactant polarisation.

    PubMed

    Aldegunde, J; Alvariño, J M; Kendrick, B K; Sáez Rábanos, V; de Miranda, M P; Aoiz, F J

    2006-11-14

    The effect of reactant polarisation on the dynamics of the title reaction at collision energies up to 1.6 eV is analysed in depth. The analysis takes advantage of two novel theoretical concepts: intrinsic reaction properties and stereodynamical portraits. Exact quantum methods are used to determine the polarisation moments that quantify the intrinsic reactant polarisation at various levels of detail, including or not product state and/or scattering angle resolution. The data is then examined with the aid of stereodynamical portraits, which facilitate the rationalisation of the stereochemical effects that are relevant for the reaction dynamics. This allows for detailed characterisations of the so-called direct and delayed reaction mechanisms.

  13. How reactants polarization can be used to change and unravel chemical reactivity.

    PubMed

    Aldegunde, Jesús; de Miranda, Marcelo P; Haigh, James M; Kendrick, Brian K; Saez-Rabanos, V; Aoiz, F Javier

    2005-07-21

    This article presents theoretical methods for the description of the directional effect of reactant rotation on the reactivity of atom-diatom systems and suggests an experiment that could be used to test theoretical predictions. The theory can be used in conjunction with both quantum reactive scattering and quasiclassical trajectory calculations, and is stated in general terms, which allows it to deal with arbitrary reactant polarizations. The illustrative results obtained for the benchmark H + D2 reaction are also presented and show that under experimentally achievable conditions one can largely control reactive cross sections and product state distributions, while at the same time gaining valuable and at times surprising information on the reaction mechanism.

  14. Estimating the effective rate of fast chemical reactions with turbulent mixing of reactants

    SciTech Connect

    Vorotilin, V. P. Yanovskii, Yu. G.

    2015-07-15

    On the basis of representation of a turbulent fluid as an aggregation of independent turbulent particles (vortexes), we derive relations for the effective rate of chemical reactions and obtain a closed system of equations describing reactions with turbulent mixing of reactants. A variant of instantaneous reactions is considered that explains the proposed approach simply. In particular, the turbulent mixing events according to this approach are uniquely related to the acts of chemical interaction, which makes it possible to exclude from consideration the mixing of inert impurities–the most difficult point of the theory formulated using classical notions. The obtained system of equations is closed without introducing arbitrarily adopted correlations, by naturally introducing the concept of effective reaction and writing the equations of conservation for both the concentrations of reactants and their volumes.

  15. Teaching illumination engineering using light pollution education kits

    NASA Astrophysics Data System (ADS)

    Walker, Constance E.; Pompea, Stephen M.; Sparks, Robert T.; Dokter, Erin

    2010-08-01

    One-third of outdoor lighting escapes unused into space, causing light pollution. Light pollution is a growing concern on many fronts: energy conservation, cost, safety, health, effects on wildlife, and our ability to view the stars. How we use illumination engineering to optimize where, when, and how light is used is of significant importance. We will discuss how NOAO's light pollution education kits are used to teach illumination engineering. In particular we will address topics associated with achieving sufficient ground coverage, color rendition, types of outdoor lighting, glare and sky glow, assessment of city lights, and task-oriented lighting.

  16. Village Green Project and Air Sensor Kits

    EPA Science Inventory

    This is a presentation for the OAQPS Teachers Workshop. Will provide a background overview on the Village Green Project and our air sensor kit for outreach, then have the teachers try putting it together.

  17. Kits for Less Than Fifty Cents

    ERIC Educational Resources Information Center

    Abruscato, Joseph; Varney, Douglas

    1975-01-01

    Describes the use of teacher-made discovery kits for use in science activities. Suggestions are provided to help teachers scrounge surplus materials from stores, small manufacturers, doctors and other potential suppliers of free materials. (PEB)

  18. Development of a forensic evidence protection kit

    NASA Astrophysics Data System (ADS)

    Acton, Brian; Kelly, Roy

    1999-02-01

    A kit has been developed for the preservation of vital forensic evidence on a suspect following a serious assault, murder or other offense where contamination may occur. This also includes the handling of firearms, explosives and/or drugs.

  19. Village Green Project and Air Sensor Kits

    EPA Science Inventory

    This is a presentation for the OAQPS Teachers Workshop. Will provide a background overview on the Village Green Project and our air sensor kit for outreach, then have the teachers try putting it together.

  20. The Research and Development of a Soluble Reactants and Products Secondary Battery System

    NASA Technical Reports Server (NTRS)

    Liu, C. C.

    1975-01-01

    A redox battery system which employs an aqueous dectrolyte is developed. Results are presented of the following experimental studies (1) measurement of the essential physical and chemical properties of the reactants and products; (2) evaluation of commerically available anion membranes as the cell separator, (3) determination of the composition and degradation mechanism of the anion membrane, and/or developing an anion membrane separator; and (4) evaluation of the performance of prototype secondary battery systems.

  1. Fluctuations and patterns in nanoscale surface reaction systems: Influence of reactant phase separation during CO oxidation

    NASA Astrophysics Data System (ADS)

    Liu, Da-Jiang; Evans, J. W.

    2007-02-01

    A realistic atomistic model is used to assess spatiotemporal behavior in nanoscale CO oxidation systems at higher pressures than for traditional ultrahigh vacuum studies. The strong influence of adspecies interactions in this regime of high reactant coverages leads to phase separation between oxygen-rich and CO-rich reactive states. Time-series studies reveal fluctuation-induced transitions between these states, as well as transitions between reactive and inactive states. In addition, we observe flickering spatial patterns with sharp boundaries.

  2. LEM-CF Premixed Tool Kit

    SciTech Connect

    2015-01-19

    The purpose of LEM-CF Premixed Tool Kit is to process premixed flame simulation data from the LEM-CF solver (https://fileshare.craft-tech.com/clusters/view/lem-cf) into a large-eddy simulation (LES) subgrid model database. These databases may be used with a user-defined-function (UDF) that is included in the Tool Kit. The subgrid model UDF may be used with the ANSYS FLUENT flow solver or other commercial flow solvers.

  3. The Topology ToolKit.

    PubMed

    Tierny, Julien; Favelier, Guillaume; Levine, Joshua A; Gueunet, Charles; Michaux, Michael

    2017-08-29

    This system paper presents the Topology ToolKit (TTK), a software platform designed for the topological analysis of scalar data in scientific visualization. While topological data analysis has gained in popularity over the last two decades, it has not yet been widely adopted as a standard data analysis tool for end users or developers. TTK aims at addressing this problem by providing a unified, generic, efficient, and robust implementation of key algorithms for the topological analysis of scalar data, including: critical points, integral lines, persistence diagrams, persistence curves, merge trees, contour trees, Morse-Smale complexes, fiber surfaces, continuous scatterplots, Jacobi sets, Reeb spaces, and more. TTK is easily accessible to end users due to a tight integration with ParaView. It is also easily accessible to developers through a variety of bindings (Python, VTK/C++) for fast prototyping or through direct, dependency-free, C++, to ease integration into pre-existing complex systems. While developing TTK, we faced several algorithmic and software engineering challenges, which we document in this paper. In particular, we present an algorithm for the construction of a discrete gradient that complies to the critical points extracted in the piecewise-linear setting. This algorithm guarantees a combinatorial consistency across the topological abstractions supported by TTK, and importantly, a unified implementation of topological data simplification for multi-scale exploration and analysis. We also present a cached triangulation data structure, that supports time efficient and generic traversals, which self-adjusts its memory usage on demand for input simplicial meshes and which implicitly emulates a triangulation for regular grids with no memory overhead. Finally, we describe an original software architecture, which guarantees memory efficient and direct accesses to TTK features, while still allowing for researchers powerful and easy bindings and extensions

  4. An efficient empirical model for microwave-induced average temperature of liquid cylindrical reactants.

    PubMed

    Yang, Chuqiao; Yakovlev, Vadim V

    2013-01-01

    Microwave-assisted chemical reactions have become very popular in preparative chemistry due to many advantages such as accelerated reaction rate, higher chemical yield and lower energy use. In dedicated equipment, however, the microwave units operate as "black boxes" keeping the role of the thermal effects in microwave-assisted chemical processes somewhat obscure. To address this issue, in this paper, we propose a simple mathematical model for computing microwave-induced temperature in a three-media cylindrical structure representing a core element of a typical microwave reactor with the reactant assumed to be stirred by convection flows. The model determines the average temperature of the reactant for the known absorbed microwave power and heating time. To illustrate its functionality, the model is used to compute time-temperature characteristics of water, ethanol, and methanol heated in the batch reactor MiniFlow 200SS. The curve calculated for water appears to be in an excellent agreement with an experiment. This confirms the hypothesis on temperature homogenization in liquid reactants in batch reactors due to convection and suggests that modeling can be helpful in clarifying and quantifying the details of microwave-assisted chemical processes.

  5. C-KIT signaling in cancer treatment.

    PubMed

    Stankov, Karmen; Popovic, Stevan; Mikov, Momir

    2014-01-01

    Tumor progression is strongly associated with the activity of receptor tyrosine kinases (RTKs) and their intracellular signal transduction pathways, which regulate several cell functions including proliferation, apoptosis, motility, adhesion and angiogenesis. Detailed structural and functional studies of RTKs, including the stem cell factor receptor c-KIT, revealed the complexity of these receptor systems and contributed to development of targeted clinical approaches with relevance in both prognosis and therapy. C-KIT signaling network has been the subject of intense research and pharmaceutical strategies to identify novel target-based approaches for cancer treatment. Evidence that c-KIT signaling promotes cell proliferation and survival, along with the frequency in which this pathway is aberrantly activated in cancer, support the current efforts to identify approaches for its efficient inhibition. C-KIT mutations are associatied with several human malignancies, such as gastrointestinal stromal tumors, acute myeloid leukemia, mast cell leukemia, and melanoma. Novel therapies are developed that target some of the identified genetic defects. It is therefore anticipated that newly-identified genetic markers will acquire a predictive value, that is, the ability to predict differential efficacy of a therapy. This review describes the evolving understanding of c-KIT/SCF axis and their downstream signaling in cancer, and the strategies for c-KIT-directed targeted cancer therapy.

  6. Orbital Maneuvering Vehicle (OMV) remote servicing kit

    NASA Technical Reports Server (NTRS)

    Brown, Norman S.

    1988-01-01

    With the design and development of the Orbital Maneuvering Vehicle (OMV) progressing toward an early 1990 initial operating capability (IOC), a new era in remote space operations will evolve. The logical progression to OMV front end kits would make available in situ satellite servicing, repair, and consummables resupply to the satellite community. Several conceptual design study efforts are defining representative kits (propellant tanks, debris recovery, module servicers); additional focus must also be placed on an efficient combination module servicer and consummables resupply kit. A remote servicer kit of this type would be designed to perform many of the early maintenance/resupply tasks in both nominal and high inclination orbits. The kit would have the capability to exchange Orbital Replacement Units (ORUs), exchange propellant tanks, and/or connect fluid transfer umbilicals. Necessary transportation system functions/support could be provided by interfaces with the OMV, Shuttle (STS), or Expendable Launch Vehicle (ELV). Specific remote servicer kit designs, as well as ground and flight demonstrations of servicer technology are necessary to prepare for the potential overwhelming need. Ground test plans should adhere to the component/system/breadboard test philosophy to assure maximum capability of one-g testing. The flight demonstration(s) would most likely be a short duration, Shuttle-bay experiment to validate servicer components requiring a micro-g environment.

  7. Orbital Maneuvering Vehicle (OMV) remote servicing kit

    NASA Technical Reports Server (NTRS)

    Brown, Norman S.

    1988-01-01

    With the design and development of the Orbital Maneuvering Vehicle (OMV) progressing toward an early 1990 initial operating capability (IOC), a new era in remote space operations will evolve. The logical progression to OMV front end kits would make available in situ satellite servicing, repair, and consummables resupply to the satellite community. Several conceptual design study efforts are defining representative kits (propellant tanks, debris recovery, module servicers); additional focus must also be placed on an efficient combination module servicer and consummables resupply kit. A remote servicer kit of this type would be designed to perform many of the early maintenance/resupply tasks in both nominal and high inclination orbits. The kit would have the capability to exchange Orbital Replacement Units (ORUs), exchange propellant tanks, and/or connect fluid transfer umbilicals. Necessary transportation system functions/support could be provided by interfaces with the OMV, Shuttle (STS), or Expendable Launch Vehicle (ELV). Specific remote servicer kit designs, as well as ground and flight demonstrations of servicer technology are necessary to prepare for the potential overwhelming need. Ground test plans should adhere to the component/system/breadboard test philosophy to assure maximum capability of one-g testing. The flight demonstration(s) would most likely be a short duration, Shuttle-bay experiment to validate servicer components requiring a micro-g environment.

  8. Niche anchorage and signaling through membrane-bound Kit-ligand/c-kit receptor are kinase independent and imatinib insensitive.

    PubMed

    Tabone-Eglinger, Séverine; Calderin-Sollet, Zuleika; Pinon, Perrine; Aebischer, Nicole; Wehrle-Haller, Monique; Jacquier, Marie-Claude; Boettiger, David; Wehrle-Haller, Bernhard

    2014-10-01

    Kit ligand (KitL) and its tyrosine kinase receptor c-kit are critical for germ cells, melanocytes, mastocytes, and hematopoietic stem cells. Alternative splicing of KitL generates membrane-bound KitL (mb-KitL) or soluble KitL, providing survival or cell migration, respectively. Here we analyzed whether c-kit can function both as an adhesion and signaling receptor to mb-KitL presented by the environmental niche. At contacts between fibroblasts and MC/9 mast cells, mb-KitL, and c-kit formed ligand/receptor clusters that formed stable complexes, which resisted dissociation by c-kit blocking mAbs and provided cell anchorage under physiological shear stresses. Clusters recruited tyrosine-phosphorylated proteins and induced spatially restricted F-actin polymerization. Mutational analysis of c-kit demonstrated kinase-independent mb-KitL/c-kit clustering, anchorage, F-actin polymerization, and Tyr567-dependent cluster phosphorylation. Kinase inhibition of c-kit by imatinib reduced cluster coalescence, but allowed cluster phosphorylation and F-actin polymerization, which required PI3K recruitment and a newly identified juxtamembrane residue. Synergies between integrin and c-kit-mediated spreading and adhesion of MC/9 cells were studied in vitro on immobilized-KitL/fibronectin surfaces. While c-kit blocking antibodies prevented spreading, imatinib blocked spreading induced by soluble- but not immobilized KitL. Thus, "mechanical" activation of c-kit provides signaling, niche-anchorage, and synergies with integrin-mediated adhesion, which is independent of kinase function and resistant to c-kit kinase inhibitors.-

  9. 21 CFR 868.1100 - Arterial blood sampling kit.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Arterial blood sampling kit. 868.1100 Section 868...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Diagnostic Devices § 868.1100 Arterial blood sampling kit. (a) Identification. An arterial blood sampling kit is a device, in kit form, used to obtain arterial blood...

  10. 21 CFR 868.1100 - Arterial blood sampling kit.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Arterial blood sampling kit. 868.1100 Section 868...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Diagnostic Devices § 868.1100 Arterial blood sampling kit. (a) Identification. An arterial blood sampling kit is a device, in kit form, used to obtain arterial blood...

  11. 21 CFR 868.1100 - Arterial blood sampling kit.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Arterial blood sampling kit. 868.1100 Section 868...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Diagnostic Devices § 868.1100 Arterial blood sampling kit. (a) Identification. An arterial blood sampling kit is a device, in kit form, used to obtain arterial blood...

  12. 21 CFR 868.1100 - Arterial blood sampling kit.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Arterial blood sampling kit. 868.1100 Section 868...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Diagnostic Devices § 868.1100 Arterial blood sampling kit. (a) Identification. An arterial blood sampling kit is a device, in kit form, used to obtain arterial blood...

  13. 21 CFR 870.1350 - Catheter balloon repair kit.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Catheter balloon repair kit. 870.1350 Section 870... repair kit. (a) Identification. A catheter balloon repair kit is a device used to repair or replace the balloon of a balloon catheter. The kit contains the materials, such as glue and balloons, necessary...

  14. 49 CFR 173.165 - Polyester resin kits.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 2 2013-10-01 2013-10-01 false Polyester resin kits. 173.165 Section 173.165... Polyester resin kits. (a) Polyester resin kits consisting of a base material component (Class 3, Packing..., according to the criteria for Class 3, applied to the base material. Additionally, polyester resin kits...

  15. 49 CFR 173.165 - Polyester resin kits.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 2 2011-10-01 2011-10-01 false Polyester resin kits. 173.165 Section 173.165... Polyester resin kits. (a) Except for transportation by aircraft, polyester resin kits consisting of a base... resin kits consisting of a base material component (Class 3, Packing Group II or III) and an...

  16. 21 CFR 870.1350 - Catheter balloon repair kit.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Catheter balloon repair kit. 870.1350 Section 870... repair kit. (a) Identification. A catheter balloon repair kit is a device used to repair or replace the balloon of a balloon catheter. The kit contains the materials, such as glue and balloons, necessary...

  17. 21 CFR 870.1350 - Catheter balloon repair kit.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Catheter balloon repair kit. 870.1350 Section 870... repair kit. (a) Identification. A catheter balloon repair kit is a device used to repair or replace the balloon of a balloon catheter. The kit contains the materials, such as glue and balloons, necessary...

  18. 21 CFR 870.1350 - Catheter balloon repair kit.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Catheter balloon repair kit. 870.1350 Section 870... repair kit. (a) Identification. A catheter balloon repair kit is a device used to repair or replace the balloon of a balloon catheter. The kit contains the materials, such as glue and balloons, necessary...

  19. 49 CFR 173.165 - Polyester resin kits.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 2 2014-10-01 2014-10-01 false Polyester resin kits. 173.165 Section 173.165... Polyester resin kits. (a) Polyester resin kits consisting of a base material component (Class 3, Packing..., according to the criteria for Class 3, applied to the base material. Additionally, polyester resin kits...

  20. 21 CFR 870.1350 - Catheter balloon repair kit.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Catheter balloon repair kit. 870.1350 Section 870... repair kit. (a) Identification. A catheter balloon repair kit is a device used to repair or replace the balloon of a balloon catheter. The kit contains the materials, such as glue and balloons, necessary...

  1. 21 CFR 868.1100 - Arterial blood sampling kit.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Arterial blood sampling kit. 868.1100 Section 868...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Diagnostic Devices § 868.1100 Arterial blood sampling kit. (a) Identification. An arterial blood sampling kit is a device, in kit form, used to obtain arterial blood...

  2. 46 CFR 184.710 - First-aid kits.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false First-aid kits. 184.710 Section 184.710 Shipping COAST... CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Miscellaneous § 184.710 First-aid kits. A vessel must carry either a first-aid kit approved under approval series 160.041 or a kit with equivalent...

  3. 46 CFR 184.710 - First-aid kits.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false First-aid kits. 184.710 Section 184.710 Shipping COAST... CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Miscellaneous § 184.710 First-aid kits. A vessel must carry either a first-aid kit approved under approval series 160.041 or a kit with equivalent...

  4. 46 CFR 184.710 - First-aid kits.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false First-aid kits. 184.710 Section 184.710 Shipping COAST... CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Miscellaneous § 184.710 First-aid kits. A vessel must carry either a first-aid kit approved under approval series 160.041 or a kit with equivalent...

  5. 46 CFR 184.710 - First-aid kits.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false First-aid kits. 184.710 Section 184.710 Shipping COAST... CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Miscellaneous § 184.710 First-aid kits. A vessel must carry either a first-aid kit approved under approval series 160.041 or a kit with equivalent...

  6. 46 CFR 184.710 - First-aid kits.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false First-aid kits. 184.710 Section 184.710 Shipping COAST... CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Miscellaneous § 184.710 First-aid kits. A vessel must carry either a first-aid kit approved under approval series 160.041 or a kit with equivalent...

  7. 19 CFR 122.132 - Sealing of aircraft liquor kits.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Sealing of aircraft liquor kits. 122.132 Section... OF THE TREASURY AIR COMMERCE REGULATIONS Aircraft Liquor Kits § 122.132 Sealing of aircraft liquor kits. (a) Sealing required. Aircraft liquor kits shall be sealed on board the aircraft by crewmembers...

  8. Entropy and chemical change. 1: Characterization of product (and reactant) energy distributions in reactive molecular collisions: Information and enthropy deficiency

    NASA Technical Reports Server (NTRS)

    Bernstein, R. B.; Levine, R. D.

    1972-01-01

    Optimal means of characterizing the distribution of product energy states resulting from reactive collisions of molecules with restricted distributions of initial states are considered, along with those for characterizing the particular reactant state distribution which yields a given set of product states at a specified total energy. It is suggested to represent the energy-dependence of global-type results in the form of square-faced bar plots, and of data for specific-type experiments as triangular-faced prismatic plots. The essential parameters defining the internal state distribution are isolated, and the information content of such a distribution is put on a quantitative basis. The relationship between the information content, the surprisal, and the entropy of the continuous distribution is established. The concept of an entropy deficiency, which characterizes the specificity of product state formation, is suggested as a useful measure of the deviance from statistical behavior. The degradation of information by experimental averaging is considered, leading to bounds on the entropy deficiency.

  9. The Fluid Dynamics Demo Kit: Part I

    NASA Astrophysics Data System (ADS)

    Flack, Karen; Underhill, Patrick; Prestridge, Kathy

    2012-11-01

    The goal of this project is to develop a fluid dynamics demonstration/experiment kit that can be used by professors and graduate students at high school outreach events. The demonstrations in the kit will be easy to use and true crowd pleasers in order to inspire understanding and pique curiosity about the physics of flow. The kits will be inexpensive, containing readily available materials so that teachers can duplicate the demonstrations and experiments. The kits will be left with the teachers as a gift from the American Physics Society. The experiments and demonstrations cover the concepts of conservation of mass, momentum, and energy, Bernoulli's equation, frictional losses and the ideal gas law. For each experiment, the teachers will receive presentation material, access to instructional videos, plus a worksheet that can be used in a high school physics classroom. This kit has been developed through the efforts of the APS-DFD Mentoring and Outreach Committee and has received funding from the APS-DFD. Work funded by the APS-DFD.

  10. Control of Oocyte Reawakening by Kit

    PubMed Central

    Castrillon, Diego H.

    2016-01-01

    In mammals, females are born with finite numbers of oocytes stockpiled as primordial follicles. Oocytes are “reawakened” via an ovarian-intrinsic process that initiates their growth. The forkhead transcription factor Foxo3 controls reawakening downstream of PI3K-AKT signaling. However, the identity of the presumptive upstream cell surface receptor controlling the PI3K-AKT-Foxo3 axis has been questioned. Here we show that the receptor tyrosine kinase Kit controls reawakening. Oocyte-specific expression of a novel constitutively-active KitD818V allele resulted in female sterility and ovarian failure due to global oocyte reawakening. To confirm this result, we engineered a novel loss-of-function allele, KitL. Kit inactivation within oocytes also led to premature ovarian failure, albeit via a contrasting phenotype. Despite normal initial complements of primordial follicles, oocytes remained dormant with arrested oocyte maturation. Foxo3 protein localization in the nucleus versus cytoplasm explained both mutant phenotypes. These genetic studies provide formal genetic proof that Kit controls oocyte reawakening, focusing future investigations into the causes of primary ovarian insufficiency and ovarian aging. PMID:27500836

  11. Reactant conversion in homogeneous turbulence: Mathematical modeling, computational validations and practical applications

    NASA Technical Reports Server (NTRS)

    Madnia, C. K.; Frankel, S. H.; Givi, P.

    1992-01-01

    Closed form analytical expressions are obtained for predicting the limited rate of reactant conversion in a binary reaction of the type F + rO yields (1 + r) Product in unpremixed homogeneous turbulence. These relations are obtained by means of a single point Probability Density Function (PDF) method based on the Amplitude Mapping Closure. It is demonstrated that with this model, the maximum rate of the reactants' decay can be conveniently expressed in terms of definite integrals of the Parabolic Cylinder Functions. For the cases with complete initial segregation, it is shown that the results agree very closely with those predicted by employing a Beta density of the first kind for an appropriately defined Shvab-Zeldovich scalar variable. With this assumption, the final results can also be expressed in terms of closed form analytical expressions which are based on the Incomplete Beta Functions. With both models, the dependence of the results on the stoichiometric coefficient and the equivalence ratio can be expressed in an explicit manner. For a stoichiometric mixture, the analytical results simplify significantly. In the mapping closure, these results are expressed in terms of simple trigonometric functions. For the Beta density model, they are in the form of Gamma Functions. In all the cases considered, the results are shown to agree well with data generated by Direct Numerical Simulations (DNS). Due to the simplicity of these expressions and because of nice mathematical features of the Parabolic Cylinder and the Incomplete Beta Functions, these models are recommended for estimating the limiting rate of reactant conversion in homogeneous reacting flows. These results also provide useful insights in assessing the extent of validity of turbulence closures in the modeling of unpremixed reacting flows. Some discussions are provided on the extension of the model for treating more complicated reacting systems including realistic kinetics schemes and multi-scalar mixing

  12. Single cell performance studies on the Fe/Cr Redox Energy Storage System using mixed reactant solutions at elevated temperature

    NASA Technical Reports Server (NTRS)

    Gahn, R. F.; Hagedorn, N. H.; Ling, J. S.

    1983-01-01

    Experimental studies in a 14.5 sq cm single cell system using mixed reactant solutions at 65 C are described. Systems were tested under isothermal conditions i.e., reactants and the cell were at the same temperature. Charging and discharging performance were evaluted by measuring watt-hour and coulombic efficiencies, voltage-current relationships, hydrogen evolution and membrane resistivity. Watt-hour efficiencies ranged from 86% at 43 ma/sq cm to 75% at 129 ma/sq cm with corresponding coulombic efficiencies of 92% and 97%, respectively. Hydrogen evolution was less than 1% of the charge coulombic capacity during charge-discharge cycling. Bismuth and bismuth-lead catalyzed chromium electrodes maintained reversible performance and low hydrogen evolution under normal and adverse cycling conditions. Reblending of the anode and cathode solutions was successfully demonstrated to compensate for osmotic volume changes. Improved performance was obtained with mixed reactant systems in comparison to the unmixed reactant systems.

  13. Reaction-Front Dynamics in a + B → C with Initially-Separated Reactants

    NASA Astrophysics Data System (ADS)

    Havlin, S.; Araujo, M.; Larralde, H.; Shehter, A.; Stanley, H. E.

    We review recent developments in the study of the diffusion reaction system of the type A + B → C in which the reactants are initially separated. We consider the case where the A and B particles are initially placed uniformly in Euclidean space at x > 0 and x < 0 respectively. We find that whereas for d ≥ 2 a single scaling exponent characterizes the width of the reaction zone, a multiscaling approach is needed to describe the one-dimensional system. We also present analytical and numerical results for the reaction rate on fractals and percolation systems.

  14. Diffusion-controlled reaction, A+B-->C, with initially separated reactants

    NASA Astrophysics Data System (ADS)

    Havlin, S.; Araujo, M.; Larralde, H.; Stanley, H. E.; Trunfio, P.

    1992-12-01

    We review recent developments in the study of diffusion-reaction systems of the type A+B→C in which reactants are initially separated. We consider two initial boundary conditions: (i) the A and B particles are initially placed uniformly in Euclidean space at x>O and x<0 respectively, and (ii) the A particles are diffusing and inserted at a given site and the B particles are static and distributed uniformly in space. We present analytical and numerical results for both systems. We consider d = 1, 2, 3 dimensional systems as well as fractal lattices.

  15. Field Test Kit for Gun Residue Detection

    SciTech Connect

    WALKER, PAMELA K.; RODACY, PHILIP J.

    2002-01-01

    One of the major needs of the law enforcement field is a product that quickly, accurately, and inexpensively identifies whether a person has recently fired a gun--even if the suspect has attempted to wash the traces of gunpowder off. The Field Test Kit for Gunshot Residue Identification based on Sandia National Laboratories technology works with a wide variety of handguns and other weaponry using gunpowder. There are several organic chemicals in small arms propellants such as nitrocellulose, nitroglycerine, dinitrotoluene, and nitrites left behind after the firing of a gun that result from the incomplete combustion of the gunpowder. Sandia has developed a colorimetric shooter identification kit for in situ detection of gunshot residue (GSR) from a suspect. The test kit is the first of its kind and is small, inexpensive, and easily transported by individual law enforcement personnel requiring minimal training for effective use. It will provide immediate information identifying gunshot residue.

  16. Shuttle Kit Freezer Refrigeration Unit Conceptual Design

    NASA Technical Reports Server (NTRS)

    Copeland, R. J.

    1975-01-01

    The refrigerated food/medical sample storage compartment as a kit to the space shuttle orbiter is examined. To maintain the -10 F in the freezer kit, an active refrigeration unit is required, and an air cooled Stirling Cycle refrigerator was selected. The freezer kit contains two subsystems, the refrigeration unit, and the storage volume. The freezer must provide two basic capabilities in one unit. One requirement is to store 215 lbs of food which is consumed in a 30-day period by 7 people. The other requirement is to store 128.3 lbs of medical samples consisting of both urine and feces. The unit can be mounted on the lower deck of the shuttle cabin, and will occupy four standard payload module compartments on the forward bulkhead. The freezer contains four storage compartments.

  17. Model Analysis ToolKit

    SciTech Connect

    Harp, Dylan R.

    2015-05-15

    MATK provides basic functionality to facilitate model analysis within the Python computational environment. Model analysis setup within MATK includes: - define parameters - define observations - define model (python function) - define samplesets (sets of parameter combinations) Currently supported functionality includes: - forward model runs - Latin-Hypercube sampling of parameters - multi-dimensional parameter studies - parallel execution of parameter samples - model calibration using internal Levenberg-Marquardt algorithm - model calibration using lmfit package - model calibration using levmar package - Markov Chain Monte Carlo using pymc package MATK facilitates model analysis using: - scipy - calibration (scipy.optimize) - rpy2 - Python interface to R

  18. Low Cost, Advanced, Integrated Microcontroller Training Kit

    NASA Astrophysics Data System (ADS)

    Somantri, Y.; Fushshilat, I.

    2017-03-01

    This paper describes the design of an AVR microcontroller training kit with a low cost and the additional feature of an integrated downloader. The main components of this device include: Microcontroller, terminal, I/O keypad, push button, LED, seven segment display, LCD, motor stepper, and sensors. The device configuration results in low cost and ease of use; this device is suitable for laboratories with limited funding. The device can also be used as a training kit for the teaching and learning of microcontrollers.

  19. FES kinase participates in KIT-ligand induced chemotaxis

    SciTech Connect

    Voisset, Edwige; Lopez, Sophie; Chaix, Amandine; Vita, Marina; George, Coralie; Dubreuil, Patrice; De Sepulveda, Paulo

    2010-02-26

    FES is a cytoplasmic tyrosine kinase activated by several membrane receptors, originally identified as a viral oncogene product. We have recently identified FES as a crucial effector of oncogenic KIT mutant receptor. However, FES implication in wild-type KIT receptor function was not addressed. We report here that FES interacts with KIT and is phosphorylated following activation by its ligand SCF. Unlike in the context of oncogenic KIT mutant, FES is not involved in wild-type KIT proliferation signal, or in cell adhesion. Instead, FES is required for SCF-induced chemotaxis. In conclusion, FES kinase is a mediator of wild-type KIT signalling implicated in cell migration.

  20. FUELS IN SOIL TEST KIT: FIELD USE OF DIESEL DOG SOIL TEST KITS

    SciTech Connect

    Unknown

    2001-05-31

    Western Research Institute (WRI) is commercializing Diesel Dog Portable Soil Test Kits for performing analysis of fuel-contaminated soils in the field. The technology consists of a method developed by WRI (U.S. Patents 5,561,065 and 5,976,883) and hardware developed by WRI that allows the method to be performed in the field (patent pending). The method is very simple and does not require the use of highly toxic reagents. The aromatic components in a soil extract are measured by absorption at 254 nm with a field-portable photometer. WRI added significant value to the technology by taking the method through the American Society for Testing and Materials (ASTM) approval and validation processes. The method is designated ASTM Method D-5831-96, Standard Test Method for Screening Fuels in Soils. This ASTM designation allows the method to be used for federal compliance activities. In FY 99, twenty-five preproduction kits were successfully constructed in cooperation with CF Electronics, Inc., of Laramie, Wyoming. The kit components work well and the kits are fully operational. In the calendar year 2000, kits were provided to the following entities who agreed to participate as FY 99 and FY 00 JSR (Jointly Sponsored Research) cosponsors and use the kits as opportunities arose for field site work: Wyoming Department of Environmental Quality (DEQ) (3 units), F.E. Warren Air Force Base, Gradient Corporation, The Johnson Company (2 units), IT Corporation (2 units), TRC Environmental Corporation, Stone Environmental, ENSR, Action Environmental, Laco Associates, Barenco, Brown and Caldwell, Dames and Moore Lebron LLP, Phillips Petroleum, GeoSyntek, and the State of New Mexico. By early 2001, ten kits had been returned to WRI following the six-month evaluation period. On return, the components of all ten kits were fully functional. The kits were upgraded with circuit modifications, new polyethylene foam inserts, and updated instruction manuals.

  1. Replacing Chlorine with Hydrogen Chloride as a Possible Reactant for Synthesis of Titanium Carbide Derived Carbon Powders for High-Technology Devices

    NASA Astrophysics Data System (ADS)

    Tallo, Indrek; Thomberg, Thomas; Jänes, Alar; Lust, Enn

    2013-12-01

    Micro- and mesoporous carbide-derived carbons were synthesized from titanium carbide (TiC) powder via gas phase reaction by using different reactants (Cl2 and HCl) within the temperature range from 700 to 1100 °C. Analysis of XRD results show that TiC-derived carbons (TiC-CDC) consist mainly of graphitic crystallites. The first-order Raman spectra showed the graphite-like absorption peaks at ~1577 cm1 and the disorder-induced peaks at ~1338 cm-1. The energy-related properties of supercapacitors based on 1 M (C2H5)3CH3NBF4 in acetonitrile and carbide-derived carbons (TiC-CDC (Cl2) and TiC-CDC (HCl)) as electrode materials were also investigated using cyclic voltammetry, electrochemical impedance spectroscopy, galvanostatic charge/discharge and constant power methods. The Ragone plots for carbide-derived carbons prepared by using different reactants (Cl2, HCl) are quite similar and at high power loads TiC-CDC (Cl2) material synthesized at 900 °C, i.e. materials with optimal porous structure, deliver higher power at constant energy.

  2. Platinum- and membrane-free swiss-roll mixed-reactant alkaline fuel cell.

    PubMed

    Aziznia, Amin; Oloman, Colin W; Gyenge, Előd L

    2013-05-01

    Eliminating the expensive and failure-prone proton exchange membrane (PEM) together with the platinum-based anode and cathode catalysts would significantly reduce the high capital and operating costs of low-temperature (<373 K) fuel cells. We recently introduced the Swiss-roll mixed-reactant fuel cell (SR-MRFC) concept for borohydride-oxygen alkaline fuel cells. We now present advances in anode electrocatalysis for borohydride electrooxidation through the development of osmium nanoparticulate catalysts supported on porous monolithic carbon fiber materials (referred to as an osmium 3D anode). The borohydride-oxygen SR-MRFC operates at 323 K and near atmospheric pressure, generating a peak power density of 1880 W m(-2) in a single-cell configuration by using an osmium-based anode (with an osmium loading of 0.32 mg cm(-2)) and a manganese dioxide gas-diffusion cathode. To the best of our knowledge, 1880 W m(-2) is the highest power density ever reported for a mixed-reactant fuel cell operating under similar conditions. Furthermore, the performance matches the highest reported power densities for conventional dual chamber PEM direct borohydride fuel cells.

  3. Unsteady Diffusion Flames: Ignition, Travel, and Burnout (SUBCORE Project: Simplified Unsteady Burning of Contained Reactants)

    NASA Technical Reports Server (NTRS)

    Fendell, Francis; Rungaldier, Harald

    1999-01-01

    An experimental apparatus for the examination of a planar, virtually strain-rate-free diffusion flame in microgravity has been designed and fabricated. Such a diffusion flame is characterized by relatively large spatial scale and high symmetry (to facilitate probing), and by relatively long fluid-residence time (to facilitate investigation of rates associated with sooting phenomena). Within the squat rectangular apparatus, with impervious, noncatalytic isothermal walls of stainless steel, a thin metallic splitter plate subdivides the contents into half-volumes. One half-volume initially contains fuel vapor diluted with an inert gas, and the other, oxidizer diluted with another inert gas-so that the two domains have equal pressure, density, and temperature. As the separator is removed, by translation in its own plane, through a tightly fitting slit in one side wall, a line ignitor in the opposite side wall initiates a triple-flame propagation across the narrow layer of combustible mixture formed near midheight in the chamber. The planar diffusion flame so emplaced is quickly disrupted in earth gravity. In microgravity, the planar flame persists, and travels ultimately into the half-volume containing the stoichiometrically deficient reactant; the flame eventually becomes extinguished owing to reactant depletion and heat loss to the walls.

  4. Operating proton exchange membrane fuel cells without external humidification of the reactant gases

    SciTech Connect

    Buechi, F.N.; Srinivasan, S.

    1997-08-01

    Operation of proton exchange membrane fuel cells (PEMFC) without external humidification of the reactant gases is advantageous for the PEMFC system, because it eliminates the need for a gas-humidification subsystem. The gas-humidification subsystem is a burden in the fuel cell system with respect to weight, complexity, cost, and parasitic power. A model for the operation of PEMFC with internal humidification of the gases is presented and the range of operating conditions for a PEMFC using dry H{sub 2}/air was investigated. The model predicts that dry air, entering at the cathode, can be fully internally humidified by the water produced by the electrochemical reaction at temperatures up to 70 C. This model was experimentally verified for cell temperatures up to 60 C by long-term operation of a PEMFC with dry gases for up to 1,800 h. The current densities, obtained at 0.6 V, were 20 to 40% lower than those measured when both gases were humidified. The water distribution in the cell, while operating with dry gases, was investigated by measuring the amount of product water on the anode and cathode sides. It was found that the back-diffusion of product water to the anode is the dominant process for water management in the cell over a wide range of operating conditions. The dominating water back-diffusion also allows internal humidification of the hydrogen reactant and prevents drying out of the anode.

  5. Fibrinogen-like protein 1, a hepatocyte derived protein is an acute phase reactant

    SciTech Connect

    Liu Zhilin; Ukomadu, Chinweike

    2008-01-25

    Fibrinogen-like protein 1 (FGL1) is a hepatocyte derived protein that is upregulated in regenerating rodent livers following partial hepatectomy. It has been implicated as a mitogen for liver cell proliferation. In this study, we show that recombinant human IL-6 induces FGL1 expression in Hep G2 cells in a pattern similar to those of acute phase reactants. Following induction of acute inflammation in rats by subcutaneous injection of turpentine oil, serum FGL1 levels are also enhanced. Although, a recent report suggests that FGL1 associates almost exclusively with the fibrin matrix, we report here that approximately 20% of the total plasma FGL1 remains free. The enhancement of FGL1 levels in vitro by IL-6 and its induction after turpentine oil injection suggest that it is an acute phase reactant. Its presence in bound and free forms in the blood also implies biological roles that extend beyond the proposed autocrine effect it has on hepatocytes during regeneration.

  6. High energy density proton exchange membrane fuel cell with dry reactant gases

    SciTech Connect

    Srinivasan, S.; Gamburzev, S.; Velev, O.A.

    1996-12-31

    Proton exchange membrane fuel cells (PEMFC) require careful control of humidity levels in the cell stack to achieve a high and stable level of performance. External humidification of the reactant gases, as in the state-of-the-art PEMFCs, increases the complexity, the weight, and the volume of the fuel cell power plant. A method for the operation of PEMFCs without external humidification (i.e., self-humidified PEMFCs) was first developed and tested by Dhar at BCS Technology. A project is underway in our Center to develop a PEMFC cell stack, which can work without external humidification and attain a performance level of a current density of 0.7 A/cm{sup 2} at a cell potential of 0.7 V, with hydrogen/air as reactants at 1 atm pressure. In this paper, the results of our efforts to design and develop a PEMFC stack requiring no external humidification will be presented. This paper focuses on determining the effects of type of electrodes, the methods of their preparation, as well as that of the membrane and electrode assembly (MEA), platinum loading and types of electrocatalyst on the performance of the PEMFC will be illustrated.

  7. Mechanisms for negative reactant ion formation in an atmospheric pressure corona discharge

    SciTech Connect

    Ewing, Robert G.; Waltman, Melanie J.

    2009-06-02

    In an effort to better understand the formation of negative reactant ions in air produced by an atmospheric pressure corona discharge source, the neutral vapors generated by the corona were introduced in varying amounts into the ionization region of an ion mobility spectrometer/mass spectrometer containing a 63Ni ionization source. With no discharge gas the predominant ions were O2- , however, upon the introduction of low levels of discharge gas the NO2- ion quickly became the dominant species. As the amount of discharge gas increased the appearance of CO3- was observed followed by the appearance of NO3-. At very high levels, NO3- species became effectively the only ion present and appeared as two peaks in the IMS spectrum, NO3- and the NO3-•HNO3 adduct, with separate mobilities. Since explosive compounds typically ionize in the presence of negative reactant ions, the ionization of an explosive, RDX, was examined in order to investigate the ionization properties with these three primary ions. It was found that RDX forms a strong adduct with both NO2- and NO3- with reduced mobility values of 1.49 and 1.44 cm2V-1s-1, respectively. No adduct was observed for RDX with CO3- although this adduct has been observed with a corona discharge mass spectrometer. It is believed that this adduct, although formed, does not have a sufficiently long lifetime (greater than 10 ms) to be observed in an ion mobility spectrometer.

  8. Understanding the electromagnetic interaction of metal organic framework reactants in aqueous solution at microwave frequencies.

    PubMed

    Laybourn, Andrea; Katrib, Juliano; Palade, Paula A; Easun, Timothy L; Champness, Neil R; Schröder, Martin; Kingman, Samuel W

    2016-02-21

    Preparation of metal organic frameworks (MOFs) via microwave heating is becoming increasingly popular due to reduced reaction times and enhanced control of MOF particle size. However, there is little understanding about the detailed interaction of the electric field portion of the wave with reactants during the synthesis of MOFs. In order to overcome this lack of fundamental understanding, information about the dielectric properties of the reactants is required. In this work the dielectric constants (ε') and loss factors (ε'') of benzene-1,4-dicarboxylic acid (H2BDC; also known as terephthalic acid) and a number of M(III) (M = metal) salts dissolved in deionized water were measured as a function of frequency, temperature and concentration and with varying anions and cations. Dielectric data confirm the aqueous M(III) salts to be strong microwave absorbers, particularly at 915 MHz. M(III) salts with mono-anionic ligands (for example chlorides and nitrates) exhibit higher losses than di-anionic salts (sulfates) demonstrating that the former are heated more effectively in an applied microwave field. Of the M(III) salts containing either singly- or doubly-charged anions, those containing Fe(III) have the highest loss indicating that they will heat more efficiently than other M(III) salts such as Cr(III) and Al(III). Interestingly, H2BDC exhibits little interaction with the electric field at microwave frequencies.

  9. Acute phase reactants predict the risk of amputation in diabetic foot infection.

    PubMed

    Akinci, Baris; Yener, Serkan; Yesil, Sena; Yapar, Nur; Kucukyavas, Yasin; Bayraktar, Firat

    2011-01-01

    prediction of amputation would aid clinicians in the management of diabetic foot infections. We aimed to assess the predictive value of baseline and post-treatment levels of acute phase reactants in the outcome of patients with diabetic foot infections. we collected data prospectively during minimum follow-up of 6 months in patients with infected diabetic foot ulcers hospitalized in Dokuz Eylul University Hospital between January 1, 2003, and January 1, 2008. After excluding patients who did not attend the hospital for follow-up visits regularly (n = 36), we analyzed data from 165 foot ulcer episodes. limb ischemia and osteomyelitis were much more frequent in patients who underwent amputation. Wagner grade, which assesses ulcer depth and the presence of osteomyelitis or gangrene, was higher in patients who needed amputation. Ulcer size was slightly larger in the amputation group. Baseline and post-treatment C-reactive protein levels, erythrocyte sedimentation rates, white blood cell counts, and platelet counts were significantly elevated in patients who underwent amputation. Albumin levels were significantly suppressed in the amputation group. Univariate analysis showed that a 1-SD increase in baseline and post-treatment C-reactive protein levels, erythrocyte sedimentation rates, and white blood cell counts and a 1-SD decrease in post-treatment albumin levels were significantly associated with increased risk of amputation. Post-treatment C-reactive protein level was strongly associated with amputation risk. circulating levels of acute phase reactants were associated with amputation risk in diabetic foot infections.

  10. Kit system in the zebrafish ovary: evidence for functional divergence of two isoforms of kit (kita and kitb) and kit ligand (kitlga and kitlgb) during folliculogenesis.

    PubMed

    Yao, Kai; Ge, Wei

    2010-06-01

    The Kit system, including Kit ligand and its receptor Kit, has critical roles in mammalian reproduction, especially in the ovary. Unlike mammalian species, two copies of genes are present in the zebrafish genome for both ligand (kitlga and kitlgb) and receptor (kita and kitb). Phylogenetic and chromosome synteny analyses suggest that these duplicated genes were likely derived from the third round of genome duplication in vertebrate evolution. All four Kit system members were expressed in the zebrafish ovary, suggesting potential roles for the system in ovarian development and function. As the first step toward understanding the Kit system in the zebrafish ovary, especially the differential roles of different forms of ligand and receptor, the present study was undertaken to analyze the temporal expression profiles of the Kit system during folliculogenesis. All members of the Kit system exhibited significant and distinct variation in expression, particularly in the periovulatory period. During the transition from the primary growth to secondary growth, kitlga showed a dramatic increase, suggesting a role for Kit signaling in follicle recruitment. During final oocyte maturation, kitlga expression significantly decreased after a transitional surge before germinal vesicle breakdown, whereas all other members exhibited an increased expression, with kitlgb and kita reaching the peak expression levels in the ovulated eggs. Our results provide strong evidence that the Kit system is involved in regulating zebrafish ovarian function and that different isoforms of Kit and Kit ligand may have differential roles in folliculogenesis. This provides an example for neofunctionalization of duplicated genes in vertebrates.

  11. PCR inhibitor removal using the NucleoSpin® DNA Clean-Up XS kit.

    PubMed

    Faber, Korie L; Person, Eric C; Hudlow, William R

    2013-01-01

    Forensic evidence samples are collected from an unlimited variety of substrates, which may contain compounds known to inhibit the polymerase chain reaction (PCR). These PCR inhibitors are co-extracted with the DNA sample and can negatively affect the DNA typing results, which can range from partial to complete inhibition of the short tandem repeat (STR) PCR. One potential solution is to remove the PCR inhibitors from the extracts prior to the STR PCR with the NucleoSpin(®) DNA Clean-Up XS kit. The kit contains a NucleoSpin(®) XS silica column that has a special funnel design of thrust rings along with a very small silica membrane, which allows for sample elution in a small volume that is appropriate for use with current STR typing kits. The NucleoSpin(®) DNA Clean-Up XS kit was optimized for the best possible DNA recovery and then evaluated for its ability to remove eight commonly encountered PCR inhibitors including: bile salt, collagen, hematin, humic acid, indigo, melanin, tannic acid and urea. Each of these PCR inhibitors was effectively removed by the NucleoSpin(®) DNA Clean-Up XS kit as demonstrated by generating more complete STR profiles from the cleaned up inhibitor samples than from the raw inhibitor samples. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  12. The receptor tyrosine kinase c-Kit controls IL-33 receptor signaling in mast cells.

    PubMed

    Drube, Sebastian; Heink, Sylvia; Walter, Sabine; Löhn, Tobias; Grusser, Mandy; Gerbaulet, Alexander; Berod, Luciana; Schons, Julia; Dudeck, Anne; Freitag, Jenny; Grotha, Stefan; Reich, Daniela; Rudeschko, Olga; Norgauer, Johannes; Hartmann, Karin; Roers, Axel; Kamradt, Thomas

    2010-05-13

    Members of the Toll/interleukin-1 receptor (TIR) family are of importance for host defense and inflammation. Here we report that the TIR-family member interleukin-33R (IL-33R) cross-activates the receptor tyrosine kinase c-Kit in human and murine mast cells. The IL-33R-induced activation of signal transducer and activator of transcription 3 (STAT3), extracellular signal-regulated kinase 1/2 (Erk1/2), protein kinase B (PKB), and Jun NH(2)-terminal kinase 1 (JNK1) depends on c-Kit and is required to elicit optimal effector functions. Costimulation with the c-Kit ligand stem cell factor (SCF) is necessary for IL-33-induced cytokine production in primary mast cells. The structural basis for this cross-activation is the complex formation between c-Kit, IL-33R, and IL-1R accessory protein (IL-1RAcP). We found that c-Kit and IL-1RAcP interact constitutively and that IL-33R joins this complex upon ligand binding. Our findings support a model in which signals from seemingly disparate receptors are integrated for full cellular responses.

  13. Preservation of Library Materials. SPEC Kit 35.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This Association of Research Libraries (ARL) kit on preservation of library materials contains: (1) descriptions of preservation programs and objectives from Boston University, the University of Michigan, University of Wisconsin, and University of California at Los Angeles; (2) a description of the Library of Congress' National Preservation…

  14. Lead Paint Test Kits Workshop: Summary Report

    EPA Science Inventory

    The U.S. Environmental Protection Agency's (EPA) Office of Research and Development (ORD) designed and conducted the Lead Paint Test Kits Workshop on October 19 and 20, 2006, at the Environmental Protection Agency's Research Triangle Park, NC campus. The workshop was conducted as...

  15. 21 CFR 876.5210 - Enema kit.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Enema kit. 876.5210 Section 876.5210 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES... device intended to instill water or other fluids into the colon through a nozzle inserted into the rectum...

  16. Test kit testing certifies soil screening accuracy

    SciTech Connect

    Neal, L.K.

    1994-08-01

    With quality controls and validation, site managers can use immunoassay with confidence. It is not enough to know which hazardous substances have contaminated a site. A manager must have an idea about how much is present and where. Traditionally, gas chromatography and mass spectrometry answered these questions, but today immunoassay soil test kits often provide more immediate results at a lower cost.

  17. Earth Is My Home, Kit I.

    ERIC Educational Resources Information Center

    1971

    Introducing the pupil to the science of ecology is the purpose of Scholastic's Earth Corps Ecology/Conservation Study Kits for grades 3-6. Simple terms are used to show how all living things are inter-related to their environment, to demonstrate the intricate and delicate balance of nature, and to point out how man's interference with nature's…

  18. Managing Corporate Annual Reports. SPEC Kit 258.

    ERIC Educational Resources Information Center

    O'Connor, Lisa, Comp.

    2000-01-01

    The purpose of the survey for this SPEC (Systems and Procedures Exchange Center) Kit was to assess the current print corporate annual report collection practices of ARL (Association of Research Libraries) libraries, describe the effects of these collections, and recommend best practices for preserving these significant historical documents. The…

  19. Networked Information Resources. SPEC Kit 253.

    ERIC Educational Resources Information Center

    Bleiler, Richard, Comp.; Plum, Terry, Comp.

    1999-01-01

    This SPEC Kit, published six times per year, examines how Association of Research Libraries (ARL) libraries have structured themselves to identify networked information resources in the market, to evaluate them for purchase, to make purchasing decisions, to publicize them, and to assess their continued utility. In the summer of 1999, the survey…

  20. Staffing the Library Website. SPEC Kit.

    ERIC Educational Resources Information Center

    Ragsdale, Kate, Comp.

    2001-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit presents the results of a survey of Association of Research Libraries (ARL) member libraries designed to gather information about who has responsibility for the development, management, and maintenance of library World Wide Web sites and to determine which combination of human resources works…

  1. Managing Corporate Annual Reports. SPEC Kit 258.

    ERIC Educational Resources Information Center

    O'Connor, Lisa, Comp.

    2000-01-01

    The purpose of the survey for this SPEC (Systems and Procedures Exchange Center) Kit was to assess the current print corporate annual report collection practices of ARL (Association of Research Libraries) libraries, describe the effects of these collections, and recommend best practices for preserving these significant historical documents. The…

  2. You Be the Chemist [Multimedia Kit].

    ERIC Educational Resources Information Center

    National Association of Chemical Distributors, Arlington, VA. Educational Foundation.

    This multimedia kit includes a teacher's manual, video, and activity packet. The unique interactive course uses safe, controlled dynamic experiments to teach kids about chemistry, the proper handling of chemicals, and responsible product stewardship. Students are asked to hypothesize about chemical substances, collect and analyze data, and share…

  3. You Be the Chemist [Multimedia Kit].

    ERIC Educational Resources Information Center

    National Association of Chemical Distributors, Arlington, VA. Educational Foundation.

    This multimedia kit includes a teacher's manual, video, and activity packet. The unique interactive course uses safe, controlled dynamic experiments to teach kids about chemistry, the proper handling of chemicals, and responsible product stewardship. Students are asked to hypothesize about chemical substances, collect and analyze data, and share…

  4. Beyond the Science Kit: Inquiry in Action.

    ERIC Educational Resources Information Center

    Saul, Wendy, Ed.; Reardon, Jeanne, Ed.

    The essays in this book are about values that are being used to drive science instruction in remarkable ways. The essays are divided into three sections. The first section contains two essays about science kits and determines the problem that the rest of the book addresses. The essays in the second section offer a glimpse of what five teachers see…

  5. Networked Information Resources. SPEC Kit 253.

    ERIC Educational Resources Information Center

    Bleiler, Richard, Comp.; Plum, Terry, Comp.

    1999-01-01

    This SPEC Kit, published six times per year, examines how Association of Research Libraries (ARL) libraries have structured themselves to identify networked information resources in the market, to evaluate them for purchase, to make purchasing decisions, to publicize them, and to assess their continued utility. In the summer of 1999, the survey…

  6. Countdown to Six Billion Teaching Kit.

    ERIC Educational Resources Information Center

    Zero Population Growth, Inc., Washington, DC.

    This teaching kit features six activities focused on helping students understand the significance of the world population reaching six billion for our society and our environment. Featured activities include: (1) History of the World: Part Six Billion; (2) A Woman's Place; (3) Baby-O-Matic; (4) Earth: The Apple of Our Eye; (5) Needs vs. Wants; and…

  7. Press kit kicks off new branding.

    PubMed

    Rees, Tom

    2004-01-01

    A smartly produced press kit resulted in unprecedented news coverage when Denver's Porter Adventist Hospital recently unveiled plans for an extensive 80 million dollars redevelopment. A news conference was held to announce this plan, along with the opening of the hospital's new emergency department. The overall effort is part of the new branding strategy of the 75-year-old hospital.

  8. Telecommunications in ARL Libraries. SPEC Kit 98.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This 11-document kit introduces the current and planned uses of telecommunications facilities in the computerized information systems of several research libraries, public libraries, and library groups contacted in 1983. The first two documents are excerpts from reports on networks: "Telecommunications: An Overview for OCLC," and…

  9. Reclassification Survey Results. SPEC Kit 16.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This kit presents the results of a survey conducted by the Association of Research Libraries (ARL) in 1974 to investigate and document various experiences of academic and research libraries in reclassifying portions of collections or entire collections and the relationship between libraries' particular classification schemes on effective…

  10. Library Systems Office Organization. SPEC Kit.

    ERIC Educational Resources Information Center

    Muir, Scott P., Comp.; Lim, Adriene, Comp.

    2002-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit presents the results of a survey of Association of Research Libraries (ARL) member libraries designed to investigate the changes in research library systems operations since 1994 and to identify future trends. A total of 70 of 124 ARL member libraries responded to the survey. A copy of the…

  11. Earth Is My Home, Kit I.

    ERIC Educational Resources Information Center

    1971

    Introducing the pupil to the science of ecology is the purpose of Scholastic's Earth Corps Ecology/Conservation Study Kits for grades 3-6. Simple terms are used to show how all living things are inter-related to their environment, to demonstrate the intricate and delicate balance of nature, and to point out how man's interference with nature's…

  12. A Tool Kit for New Chairs

    ERIC Educational Resources Information Center

    Munro, Stuart

    2006-01-01

    Many chairs come to their position without the benefit of previous experience in the role of leading an academic department. The American Association of Chairs of Departments of Psychiatry (AACDP) identified the need for a manual designed to help a new chair get started. The "tool kit" that emerged contains sections that describe the AACDP,…

  13. SunWise[R] Meteorologist Tool Kit

    ERIC Educational Resources Information Center

    US Environmental Protection Agency, 2007

    2007-01-01

    The SunWise Program is designed to help meteorologists raise sun safety awareness by addressing the science of the sun, the risk of overexposure to its ultraviolet (UV) radiation, and what students and their families can do to protect themselves from overexposure. This Tool Kit has been designed for use all over the United States and its…

  14. Teacher to Teacher: Take-Home Kits.

    ERIC Educational Resources Information Center

    Franklin, Joyce; Krebill, Joyce

    1993-01-01

    Describes a kit containing mathematical enrichment activities and activity evaluation sheets that kindergarten students can complete at home. Thirteen sample activities involving weather, pattern recognition, counting, money, estimation, observation skills, weight, temperature, measurement, and time are included. Suggestions for utilization, care,…

  15. Teen PACK: Population Awareness Campaign Kit.

    ERIC Educational Resources Information Center

    Zero Population Growth, Inc., Washington, DC.

    This packet of instructional materials is designed to teach teenagers about the effects of overpopulation on the world and on the individual. Information is presented in three related booklets. The first of the three parts of the "Teen Population Awareness Campaign Kit," illustrates overpopulation through profiles of teens living in…

  16. Post-Tenure Review. SPEC Kit 261.

    ERIC Educational Resources Information Center

    Hook, Sara Anne, Comp.; Lees, N. Doug, Comp.; Powers, Gerald, Comp.

    2000-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit reports results of a survey of ARL (Association of Research Libraries) that examined post-tenure review of librarians throughout higher education in North America. The purpose of the survey was to identify which institutions apply post-tenure or post-continuing appointment review to library…

  17. Electronic Reference Service. SPEC Kit 251.

    ERIC Educational Resources Information Center

    Goetsch, Lori, Comp.

    1999-01-01

    The goals of this SPEC Kit were to report on the extent to which ARL (Association of Research Libraries) libraries provide electronic reference services and to offer a snapshot of the types of users reached, questions received, policies established, data-gathering techniques utilized, and innovations implemented. The first section of the kit…

  18. Library Systems Office Organization. SPEC Kit.

    ERIC Educational Resources Information Center

    Muir, Scott P., Comp.; Lim, Adriene, Comp.

    2002-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit presents the results of a survey of Association of Research Libraries (ARL) member libraries designed to investigate the changes in research library systems operations since 1994 and to identify future trends. A total of 70 of 124 ARL member libraries responded to the survey. A copy of the…

  19. Haitian Component Bibliography. Migrant Heritage Studies Kit.

    ERIC Educational Resources Information Center

    Roark-Calnek, Sue, Comp.

    This 587-item annotated bibliography, designed as a supplement to the Haitian Component of the Migrant Heritage Studies Kit, provides access to additional information, including audiovisual materials, on resources on Haiti and Haitian immigrants, published between 1877 and 1984. Part I is a "General Bibliography" which includes 313…

  20. Defense Acquisition University Program Managers Tool Kit

    DTIC Science & Technology

    2011-01-01

    International Standards Organization ( ISO ) registration of a supplier’s quality system since there have been instances where ISO 9001 -registered supplier...58 Quality Management Systems (Defense Acquisition Guidebook...facilities and equipment. DAU PROGRAM MANAGERS TOOL KIT 59 Quality Management Systems (see Defense Acquisition Guidebook) • The PM should allow

  1. Managerial and Technical Specialists. SPEC Kit 20.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This kit is compiled from documentation received as a result of a 1975 Association of Research Libraries (ARL) survey on the use of specialists in member libraries; updated materials gathered in 1978 are included. Survey data from 64 ARL members indicate wide utilization of various managerial and technical specialists. Of these, personnel…

  2. Thermal protection system flight repair kit

    NASA Technical Reports Server (NTRS)

    1979-01-01

    A thermal protection system (TPS) flight repair kit required for use on a flight of the Space Transportation System is defined. A means of making TPS repairs in orbit by the crew via extravehicular activity is discussed. A cure in place ablator, a precured ablator (large area application), and packaging design (containers for mixing and dispensing) for the TPS are investigated.

  3. Computerized Online Bibliographic Searching. SPEC Kit #154.

    ERIC Educational Resources Information Center

    Hocker, Susan

    For this kit, 106 Association of Research Libraries (ARL) academic libraries were surveyed concerning: (1) current administration/organization; (2) evaluation; (3) patron relations; (4) services; and (5) the impact of online searching on collections. Responses were received from 83 libraries, many of which contributed sample materials. Analyses of…

  4. Learning Disabilities In-Service Training Kit.

    ERIC Educational Resources Information Center

    Simpson, Bickley

    Included in the training kit for teachers in the area of learning disabilities are materials developed by Project Lighthouse for experimental field usage to test the materials. The problem of educating children with learning disabilities is summarized, as is Piaget's model of logical activity. The major divisions of the text then deal with the…

  5. Lead Paint Test Kits Workshop: Summary Report

    EPA Science Inventory

    The U.S. Environmental Protection Agency's (EPA) Office of Research and Development (ORD) designed and conducted the Lead Paint Test Kits Workshop on October 19 and 20, 2006, at the Environmental Protection Agency's Research Triangle Park, NC campus. The workshop was conducted as...

  6. Career and Occupational Development Kit. Instruction Manual.

    ERIC Educational Resources Information Center

    Education Commission of the States, Denver, CO. National Assessment of Educational Progress.

    This manual is part of a kit consisting of four documents which bring together different types of items that measure a number of career and occupational development (COD) objectives developed by the National Assessment of Educational Progress (NAEP). (NAEP--which completed a national survey measuring the achievement of knowledge, skills,…

  7. Library Photocopy Operations. SPEC Kit 209.

    ERIC Educational Resources Information Center

    Almony, Robert A., Jr., Comp.; O'Brien, Francis, Comp.

    The kit and flyer examine library photocopy operations, including services, personnel, equipment, and financial management practices by member institutions of the Association of Research Libraries (ARL). To find out about these operations, ARL surveyed its 112 members, and received 93 replies. Forty-nine academic libraries (58%) described their…

  8. Career and Occupational Development Kit. Instruction Manual.

    ERIC Educational Resources Information Center

    Education Commission of the States, Denver, CO. National Assessment of Educational Progress.

    This manual is part of a kit consisting of four documents which bring together different types of items that measure a number of career and occupational development (COD) objectives developed by the National Assessment of Educational Progress (NAEP). (NAEP--which completed a national survey measuring the achievement of knowledge, skills,…

  9. Preservation of Library Materials. SPEC Kit 35.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This Association of Research Libraries (ARL) kit on preservation of library materials contains: (1) descriptions of preservation programs and objectives from Boston University, the University of Michigan, University of Wisconsin, and University of California at Los Angeles; (2) a description of the Library of Congress' National Preservation…

  10. Beyond the Science Kit: Inquiry in Action.

    ERIC Educational Resources Information Center

    Saul, Wendy, Ed.; Reardon, Jeanne, Ed.

    The essays in this book are about values that are being used to drive science instruction in remarkable ways. The essays are divided into three sections. The first section contains two essays about science kits and determines the problem that the rest of the book addresses. The essays in the second section offer a glimpse of what five teachers see…

  11. Lifeprints ESL for Adults: Literacy. [Multimedia Kit].

    ERIC Educational Resources Information Center

    Cunningham Florez, MaryAnn

    Lifeprints is a four-level program that helps adult English- as-a-Second-Language (ESL) students gain the English language skills they need to participate effectively at work and in their communities. This multimedia kit contains the first level in the series. Level one is divided into six units: "Welcome to English Class"; "Personal Information";…

  12. Reclassification Survey Results. SPEC Kit 16.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This kit presents the results of a survey conducted by the Association of Research Libraries (ARL) in 1974 to investigate and document various experiences of academic and research libraries in reclassifying portions of collections or entire collections and the relationship between libraries' particular classification schemes on effective…

  13. Managerial and Technical Specialists. SPEC Kit 20.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This kit is compiled from documentation received as a result of a 1975 Association of Research Libraries (ARL) survey on the use of specialists in member libraries; updated materials gathered in 1978 are included. Survey data from 64 ARL members indicate wide utilization of various managerial and technical specialists. Of these, personnel…

  14. Managing Printing Services. SPEC Kit 254.

    ERIC Educational Resources Information Center

    Blixrud, Julia C., Comp.

    2000-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit reports results of a survey that examined how Association of Research Libraries (ARL) members currently provide printing services to their users and how the costs of those services are covered. Of the 122 members surveyed, 62 responded. Included in the responses was information on main, law,…

  15. Gifts & Exchange Functions. SPEC Kit 28.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This collection contains data from a 1976 survey on organization, staffing, and operation of the gifts and exchange functions of member libraries of the Association of Research Libraries (ARL) and 26 primary source documents. Source documents in the kit include: (1) annual reports from Columbia University, University of North Carolina, and Ohio…

  16. The Interview Process. SPEC Kit 260.

    ERIC Educational Resources Information Center

    Frank, Heidi, Comp.; Nicholson, Shawn, Comp.; Dickson, Laura, Comp.; Miller, Terri Tickle, Comp.

    2000-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit reports results of a survey of ARL (Association of Research Libraries) that examined the nature and structure of the interview process at large research and academic libraries in the United States and Canada. By determining the nature and structure of the interview, it is hoped that candidates…

  17. Library Development and Fundraising. SPEC Kit 193.

    ERIC Educational Resources Information Center

    Claassen, Lynda Corey, Comp.

    This kit developed by the Systems and Procedures Exchange Center (SPEC) of the Association of Research Libraries (ARL) presents results of a survey of development and fundraising activities in libraries that are members of ARL, as well as some sample documents used in connection with development and fundraising. Eighty-seven institutions…

  18. Reference Service Statistics & Assessment. SPEC Kit.

    ERIC Educational Resources Information Center

    Novotny, Eric, Comp.

    2002-01-01

    This SPEC (Systems and Procedures Exchange Center) Kit presents the results of a survey of Association of Research Libraries (ARL) member libraries designed to document how ARL member libraries are collecting, using, and modifying their data on reference service transactions. A total of 74 of 124 ARL member libraries responded to the survey. A…

  19. 49 CFR 173.161 - Chemical kits and first aid kits.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... so there will be no possibility of the mixture of contents causing dangerous evolution of heat or gas... (Carriage by highway) of this subchapter except § 177.817 (Shipping papers). Kits that meet the definition...

  20. Test/QA Plan for Verification of Microcystin Test Kits

    EPA Science Inventory

    Microcystin test kits are used to quantitatively measure total microcystin in recreational waters. These test kits are based on enzyme-linked immunosorbent assays (ELISA) with antibodies that bind specifically to microcystins or phosphate activity inhibition where the phosphatas...

  1. 21 CFR 872.3570 - OTC denture repair kit.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...) MEDICAL DEVICES DENTAL DEVICES Prosthetic Devices § 872.3570 OTC denture repair kit. (a) Identification. An OTC denture repair kit is a device consisting of a material, such as a resin monomer system of...

  2. Test/QA Plan for Verification of Microcystin Test Kits

    EPA Science Inventory

    Microcystin test kits are used to quantitatively measure total microcystin in recreational waters. These test kits are based on enzyme-linked immunosorbent assays (ELISA) with antibodies that bind specifically to microcystins or phosphate activity inhibition where the phosphatas...

  3. Synthesis, Characterization and Application of Cobalt Incorporated Mesoporous KIT-6 for the Visible Light Assisted Degradation of Methylene Blue.

    PubMed

    Sanjini, N S; Winston, B; Velmathi, S

    2015-09-01

    Highly ordered mesoporous three dimensional la3d cobalt doped KIT-6 (Co-KIT-6) material was synthesized by the simple impregnation method and characterized by various physicochemical techniques such as X-ray diffraction, N2 adsorption-desorption isotherm, diffuse reflectance spectroscopy, Fourier transform infra-red spectroscopy and scanning electron microscopy. Larger pore diameter tuned with a narrow size distribution was observed for prepared Co-KIT-6. A series of Co-KIT-6 with different weight percentages solutions were prepared and the photocatalytic efficacy of the samples for degradation of methylene blue was studied under UV and visible light using UV-visible spectrophotometer. It was found that the catalytic activity depends on the amount of cobalt incorporated into the material. The complete degradation of dye was observed within 50 min. Different amount of catalyst and reaction time was considered for the optimization studies.

  4. C-kit overexpression correlates with KIT gene copy numbers increases in phyllodes tumors of the breast.

    PubMed

    Liu, Junjun; Liu, Xiaozhen; Feng, Xiaolong; Liu, Jian; Lv, Shuhua; Zhang, Wei; Niu, Yun

    2015-01-01

    We determined c-kit expression in the stroma and epithelia of benign, borderline, and malignant phyllodes tumors (PTs), respectively, as well as the relationship between c-kit expression in stromal elements and KIT gene copy number variations (CNVs). To assess c-kit expression and KIT CNVs, 348 PT cases were studied: 120 (34.4 %) benign cases, 115 (33.1 %) borderline cases, and 113 (32.5 %) malignant cases. All of these cases were evaluated for c-kit (CD117) expression using immunohistochemistry. Forty-two cases (29 c-kit-positive in the stromal cells cases and 13 negative cases) were investigated for KIT gene CNVs via genomic polymerase chain reaction (PCR). The overall rate of c-kit positivity in the stroma was 46.8 %, as well as 24.2, 53.1, and 64.6 %, respectively, in PTs of three different grades. However, in the majority of cases, the epithelia were c-kit positive (98.2 %), and the positivity was 100, 99.1, and 95 % in PTs of three different grades, respectively. There was a significant change in the expression of c-kit in the stroma and epithelia according to grade (P < 0.001, P = 0.014). From the genomic PCR results, we can confirm that c-kit positivity in the stroma is directly correlated with KIT gene copy numbers increases (P = 0.003, P = 0.041). We demonstrated that c-kit expression in the stroma of PTs is positively associated with malignancy. c-Kit epithelial positivity was inversely correlated with PTs malignancy. c-Kit overexpression in the stroma was related to KIT gene copy numbers increases.

  5. L576P KIT mutation in anal melanomas correlates with KIT protein expression and is sensitive to specific kinase inhibition.

    PubMed

    Antonescu, Cristina R; Busam, Klaus J; Francone, Todd D; Wong, Grace C; Guo, Tianhua; Agaram, Narasimhan P; Besmer, Peter; Jungbluth, Achim; Gimbel, Mark; Chen, Chin-Tung; Veach, Darren; Clarkson, Bayard D; Paty, Philip B; Weiser, Martin R

    2007-07-15

    Activating mutations in either BRAF or NRAS are seen in a significant number of malignant melanomas, but their incidence appears to be dependent to ultraviolet light exposure. Thus, BRAF mutations have the highest incidence in non-chronic sun damaged (CSD), and are uncommon in acral, mucosal and CSD melanomas. More recently, activating KIT mutations have been described in rare cases of metastatic melanoma, without further reference to their clinical phenotypes. This finding is intriguing since KIT expression is downregulated in most melanomas progressing to more aggressive lesions. In this study, we investigated a group of anal melanomas for the presence of BRAF, NRAS, KIT and PDGFRA mutations. A heterozygous KIT exon 11 L576P substitution was identified in 3 of 20 cases tested. The 3 KIT mutation-carrying tumors were strongly immunopositive for KIT protein. No KIT mutations were identified in tumors with less than 4+ KIT immunostaining. NRAS mutation was identified in one tumor. No BRAF or PDGFRA mutations were identified in either KIT positive or negative anal melanomas. In vitro drug testing of stable transformant Ba/F3 KIT(L576P) mutant cells showed sensitivity for dasatinib (previously known as BMS-354825), a dual SRC/ABL kinase inhibitor, and imatinib. However, compared to an imatinib-sensitive KIT mutant, dasatinib was potent at lower doses than imatinib in the KIT(L576P) mutant. These results suggest that a subset of anal melanomas show activating KIT mutations, which are susceptible for therapy with specific kinase inhibitors.

  6. c-kit+AT2R+ Bone Marrow Mononuclear Cell Subset Is a Superior Subset for Cardiac Protection after Myocardial Infarction

    PubMed Central

    Du, Mingjun; Zhang, Wentian; Wang, Chenxi; Lian, Feng; Xue, Song

    2016-01-01

    Although the bone marrow mononuclear cell (BMMNC) is known as an ideal cell type for cell-based therapy for MI treatment, the effective subpopulation still remains unknown. Our study aimed at identifying the optimal subset of BMMNCs suited for cardiac regeneration. In this study, we observed that MI led to (i) a significant increase of the c-kit+AT2R+ BMMNC subpopulation in mice and (ii) a modest increase of AT2R+ BMMNCs in humans. c-kit+AT2R+ and c-kit+AT2R− BMMNC subpopulations were obtained from mice after MI. Then, we cocultured cardiac H9C2 cells with c-kit+AT2R+, c-kit+AT2R−, and unfractionated BMMNCs; finally, we found that the c-kit+AT2R+ subset is superior to the c-kit+AT2R− subset in improving cardiomyocyte protection in vitro. Of note, c-kit+AT2R+ BMMNCs showed a more robust migration capacity than c-kit+AT2R− and unfractionated BMMNCs in vitro and in vivo. Additionally, compared to c-kit+AT2R− and unfractionated BMMNCs, intravenous transplantation of c-kit+AT2R+ BMMNC resulted in smaller infarct size and lower levels of inflammatory reactions in heart tissue, leading to a higher global heart function improvement. In conclusion, our results indicate that the c-kit+AT2R+ BMMNC subpopulation exerts a protective effect against MI and shows promising therapeutic possibilities with regard to the treatment of ischemic heart disease. PMID:27429622

  7. Concentration Field of Reactants and Products Species in a Reacting Vortex Ring

    NASA Technical Reports Server (NTRS)

    Chen, Shin-Juh; Dahm, Werner J. A.; Silver, Joel A.; Tryggvason, Gretar; Brooker, J. (Technical Monitor)

    2001-01-01

    The proposed paper will present experimental and numerical results on the concentration fields of both reactants and products species in a reacting vortex ring that is generated from the interaction between a diffusion flame and a laminar vortex ring. Flame-vortex interactions are canonical configurations used to study the underlying processes occurring in complicated turbulent reacting flows. This type of configuration contains many of the fundamental aspects of the coupling between fluid dynamics and combustion that could be investigated with more controllable conditions than are possible under direct investigations of turbulent flames. The current configuration has been studied experimentally by Chen and Dahm and Chen et al. under microgravity conditions, and by Park and Shin, and You et al. under normal gravity conditions. This configuration is similar to that used in the analyses of Karagozian and Manda of their 2-D vortex pair in which both fuel and entrained oxidizer are present. The vortex ring used in this study is generated by issuing methane into an air environment through the exit of an axisymmetric nozzle. The experiments were conducted under microgravity conditions in order to remove the undesirable effects of buoyancy that can affect both the flame structure and ring dynamics resulting in possibly asymmetric and nonrepeatable interactions. The experimental technique of diode laser wavelength modulation spectroscopy (WMS) is used to measure concentration fields of reactants, CH4 and O2, products, H2O, CO2, OH, and temperature fields which can be inferred from either line pairs of O2 or OH lines. This technique has been investigated previously by Silver and Bomse et al. This is the first time that the technique has been applied to reacting vortex rings under microgravity conditions. The effect of ring circulation and fuel volume on the species concentration fields will be investigated. The experimental results will be compared to the current numerical

  8. Convenient Preparation of [(68)Ga]DKFZ-PSMA-11 Using a Robust Single-Vial Kit and Demonstration of Its Clinical Efficacy.

    PubMed

    Satpati, Drishty; Shinto, Ajit; Kamaleshwaran, K K; Sane, Surekha; Banerjee, Sharmila

    2016-06-01

    [(68)Ga]DKFZ-PSMA-11 has proved to be an important diagnostic radiotracer for targeting prostate-specific membrane antigen (PSMA) overexpression in both recurrent prostate cancer (PC) and relevant metastatic sites. However, the widespread, routine clinical use of such a potential radiopharmaceutical demands availability of a ready-to-use kit formulation to enable convenient radiopharmaceutical preparation. Herein, we report the development of a freeze-dried kit vial for the formulation of [(68)Ga]DKFZ-PSMA-11 and its clinical use in patients using a "shake-bake-inject" methodology. The freeze-dried kit vial was developed after optimization of ligand content (PSMA-11) and pH conditions. The kit was formulated using (68)Ga from two different commercially available generators. Positron emission tomography/X-ray computed tomography (PET/CT) images of PC patients were obtained using the kit-formulated radiotracer. [(68)Ga]DKFZ-PSMA-11 was prepared in >98 % radiochemical yield and purity using the freeze-dried kit vials. Kits were optimized for the preparation of four patient doses. The clinical utility was evaluated in patients with histologically confirmed prostate cancer, and the images were of good quality as well as conforming to tumor marker and clinical expectations. The development of a simple and ready-to-use freeze-dried DKFZ-PSMA-11 kit for the preparation of Ga-68-based radiotracers constitutes a major step towards the expedition of the widespread and economical screening of PC patients.

  9. 46 CFR 121.710 - First-aid kits.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false First-aid kits. 121.710 Section 121.710 Shipping COAST... SYSTEMS AND EQUIPMENT Miscellaneous § 121.710 First-aid kits. A vessel must carry either a first-aid kit... kits, the contents must be stowed in a suitable, watertight container that is marked “First-Aid Kit”....

  10. 46 CFR 121.710 - First-aid kits.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false First-aid kits. 121.710 Section 121.710 Shipping COAST... SYSTEMS AND EQUIPMENT Miscellaneous § 121.710 First-aid kits. A vessel must carry either a first-aid kit... kits, the contents must be stowed in a suitable, watertight container that is marked “First-Aid Kit”....

  11. 46 CFR 121.710 - First-aid kits.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false First-aid kits. 121.710 Section 121.710 Shipping COAST... SYSTEMS AND EQUIPMENT Miscellaneous § 121.710 First-aid kits. A vessel must carry either a first-aid kit... kits, the contents must be stowed in a suitable, watertight container that is marked “First-Aid Kit”....

  12. 46 CFR 121.710 - First-aid kits.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false First-aid kits. 121.710 Section 121.710 Shipping COAST... SYSTEMS AND EQUIPMENT Miscellaneous § 121.710 First-aid kits. A vessel must carry either a first-aid kit... kits, the contents must be stowed in a suitable, watertight container that is marked “First-Aid Kit”....

  13. 46 CFR 121.710 - First-aid kits.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false First-aid kits. 121.710 Section 121.710 Shipping COAST... SYSTEMS AND EQUIPMENT Miscellaneous § 121.710 First-aid kits. A vessel must carry either a first-aid kit... kits, the contents must be stowed in a suitable, watertight container that is marked “First-Aid Kit”....

  14. 21 CFR 880.5760 - Chemical cold pack snakebite kit.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Chemical cold pack snakebite kit. 880.5760 Section... Therapeutic Devices § 880.5760 Chemical cold pack snakebite kit. (a) Identification. A chemical cold pack snakebit kit is a device consisting of a chemical cold pack and tourniquet used for first-aid treatment...

  15. 40 CFR 745.88 - Recognized test kits.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... criteria in paragraph (c) of this section. (b) No other test kits will be recognized until they are tested... response criteria in paragraph (c)(1) of this section. The recognition of kits that meet only this criteria... in paragraph (c) of this section. (4) After September 1, 2010, test kits must meet both of...

  16. 21 CFR 880.5760 - Chemical cold pack snakebite kit.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Chemical cold pack snakebite kit. 880.5760 Section... Therapeutic Devices § 880.5760 Chemical cold pack snakebite kit. (a) Identification. A chemical cold pack snakebit kit is a device consisting of a chemical cold pack and tourniquet used for first-aid treatment...

  17. 21 CFR 880.5760 - Chemical cold pack snakebite kit.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Chemical cold pack snakebite kit. 880.5760 Section... Therapeutic Devices § 880.5760 Chemical cold pack snakebite kit. (a) Identification. A chemical cold pack snakebit kit is a device consisting of a chemical cold pack and tourniquet used for first-aid treatment...

  18. 40 CFR 745.88 - Recognized test kits.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... criteria in paragraph (c) of this section. (b) No other test kits will be recognized until they are tested... response criteria in paragraph (c)(1) of this section. The recognition of kits that meet only this criteria... in paragraph (c) of this section. (4) After September 1, 2010, test kits must meet both of...

  19. 19 CFR 122.132 - Sealing of aircraft liquor kits.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... protection. (c) Seals to be used. Aircraft liquor kits shall be sealed with serially numbered, Customs... 19 Customs Duties 1 2011-04-01 2011-04-01 false Sealing of aircraft liquor kits. 122.132 Section... OF THE TREASURY AIR COMMERCE REGULATIONS Aircraft Liquor Kits § 122.132 Sealing of aircraft...

  20. 32 CFR 105.12 - SAFE Kit collection and preservation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 1 2013-07-01 2013-07-01 false SAFE Kit collection and preservation. 105.12..., MILITARY AND CIVILIAN SEXUAL ASSAULT PREVENTION AND RESPONSE PROGRAM PROCEDURES § 105.12 SAFE Kit... ability to elect a SAFE Kit in addition to the general medical management related to sexual...

  1. 19 CFR 122.132 - Sealing of aircraft liquor kits.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... protection. (c) Seals to be used. Aircraft liquor kits shall be sealed with serially numbered, Customs... 19 Customs Duties 1 2014-04-01 2014-04-01 false Sealing of aircraft liquor kits. 122.132 Section... OF THE TREASURY AIR COMMERCE REGULATIONS Aircraft Liquor Kits § 122.132 Sealing of aircraft...

  2. 19 CFR 122.132 - Sealing of aircraft liquor kits.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... protection. (c) Seals to be used. Aircraft liquor kits shall be sealed with serially numbered, Customs... 19 Customs Duties 1 2012-04-01 2012-04-01 false Sealing of aircraft liquor kits. 122.132 Section... OF THE TREASURY AIR COMMERCE REGULATIONS Aircraft Liquor Kits § 122.132 Sealing of aircraft...

  3. 21 CFR 880.5760 - Chemical cold pack snakebite kit.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Chemical cold pack snakebite kit. 880.5760 Section... Therapeutic Devices § 880.5760 Chemical cold pack snakebite kit. (a) Identification. A chemical cold pack snakebit kit is a device consisting of a chemical cold pack and tourniquet used for first-aid treatment...

  4. 21 CFR 880.5760 - Chemical cold pack snakebite kit.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Chemical cold pack snakebite kit. 880.5760 Section... Therapeutic Devices § 880.5760 Chemical cold pack snakebite kit. (a) Identification. A chemical cold pack snakebit kit is a device consisting of a chemical cold pack and tourniquet used for first-aid treatment...

  5. 40 CFR 745.88 - Recognized test kits.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... criteria in paragraph (c) of this section. (b) No other test kits will be recognized until they are tested... response criteria in paragraph (c)(1) of this section. The recognition of kits that meet only this criteria... in paragraph (c) of this section. (4) After September 1, 2010, test kits must meet both of...

  6. 19 CFR 122.132 - Sealing of aircraft liquor kits.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... protection. (c) Seals to be used. Aircraft liquor kits shall be sealed with serially numbered, Customs... 19 Customs Duties 1 2013-04-01 2013-04-01 false Sealing of aircraft liquor kits. 122.132 Section... OF THE TREASURY AIR COMMERCE REGULATIONS Aircraft Liquor Kits § 122.132 Sealing of aircraft...

  7. Working with Self-Injurious Adolescents Using the Safe Kit

    ERIC Educational Resources Information Center

    Moyer, Michael

    2008-01-01

    This article offers a guide for using the Safe Kit when working with clients who self-injure. The Safe Kit can be used as a supplement to more traditional approaches to counseling and offers clients alternatives to self-injury when they need alternatives the most. The Safe Kit works under the assumption that individuals differ in the meaning they…

  8. 21 CFR 878.3925 - Plastic surgery kit and accessories.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Plastic surgery kit and accessories. 878.3925... (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Prosthetic Devices § 878.3925 Plastic surgery kit and accessories. (a) Identification. A plastic surgery kit and accessories is a device intended to...

  9. 21 CFR 878.3925 - Plastic surgery kit and accessories.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Plastic surgery kit and accessories. 878.3925... (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Prosthetic Devices § 878.3925 Plastic surgery kit and accessories. (a) Identification. A plastic surgery kit and accessories is a device intended to...

  10. 21 CFR 878.3925 - Plastic surgery kit and accessories.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Plastic surgery kit and accessories. 878.3925... (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Prosthetic Devices § 878.3925 Plastic surgery kit and accessories. (a) Identification. A plastic surgery kit and accessories is a device intended to...

  11. 33 CFR 144.01-30 - First-aid kit.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false First-aid kit. 144.01-30 Section...) OUTER CONTINENTAL SHELF ACTIVITIES LIFESAVING APPLIANCES Manned Platforms § 144.01-30 First-aid kit. On each manned platform a first-aid kit approved by the Commandant or the U.S. Bureau of Mines shall...

  12. 46 CFR 169.725 - First aid kit.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false First aid kit. 169.725 Section 169.725 Shipping COAST... Control, Miscellaneous Systems, and Equipment § 169.725 First aid kit. Each vessel must carry an approved first aid kit, constructed and fitted in accordance with subpart 160.041 of this chapter....

  13. 46 CFR 169.725 - First aid kit.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false First aid kit. 169.725 Section 169.725 Shipping COAST... Control, Miscellaneous Systems, and Equipment § 169.725 First aid kit. Each vessel must carry an approved first aid kit, constructed and fitted in accordance with subpart 160.041 of this chapter....

  14. 46 CFR 108.707 - First aid kit.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false First aid kit. 108.707 Section 108.707 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Miscellaneous Equipment § 108.707 First aid kit. Each unit must have a first-aid kit approved by the Mine...

  15. 33 CFR 144.01-30 - First-aid kit.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false First-aid kit. 144.01-30 Section...) OUTER CONTINENTAL SHELF ACTIVITIES LIFESAVING APPLIANCES Manned Platforms § 144.01-30 First-aid kit. On each manned platform a first-aid kit approved by the Commandant or the U.S. Bureau of Mines shall...

  16. 33 CFR 144.01-30 - First-aid kit.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false First-aid kit. 144.01-30 Section...) OUTER CONTINENTAL SHELF ACTIVITIES LIFESAVING APPLIANCES Manned Platforms § 144.01-30 First-aid kit. On each manned platform a first-aid kit approved by the Commandant or the U.S. Bureau of Mines shall...

  17. 46 CFR 108.707 - First aid kit.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false First aid kit. 108.707 Section 108.707 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Miscellaneous Equipment § 108.707 First aid kit. Each unit must have a first-aid kit approved by the Mine...

  18. 46 CFR 169.725 - First aid kit.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false First aid kit. 169.725 Section 169.725 Shipping COAST... Control, Miscellaneous Systems, and Equipment § 169.725 First aid kit. Each vessel must carry an approved first aid kit, constructed and fitted in accordance with subpart 160.041 of this chapter....

  19. 46 CFR 108.707 - First aid kit.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false First aid kit. 108.707 Section 108.707 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Miscellaneous Equipment § 108.707 First aid kit. Each unit must have a first-aid kit approved by the Mine...

  20. 46 CFR 169.725 - First aid kit.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false First aid kit. 169.725 Section 169.725 Shipping COAST... Control, Miscellaneous Systems, and Equipment § 169.725 First aid kit. Each vessel must carry an approved first aid kit, constructed and fitted in accordance with subpart 160.041 of this chapter....

  1. 46 CFR 169.725 - First aid kit.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false First aid kit. 169.725 Section 169.725 Shipping COAST... Control, Miscellaneous Systems, and Equipment § 169.725 First aid kit. Each vessel must carry an approved first aid kit, constructed and fitted in accordance with subpart 160.041 of this chapter....

  2. 33 CFR 144.01-30 - First-aid kit.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false First-aid kit. 144.01-30 Section...) OUTER CONTINENTAL SHELF ACTIVITIES LIFESAVING APPLIANCES Manned Platforms § 144.01-30 First-aid kit. On each manned platform a first-aid kit approved by the Commandant or the U.S. Bureau of Mines shall...

  3. 21 CFR 878.3925 - Plastic surgery kit and accessories.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Plastic surgery kit and accessories. 878.3925... (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Prosthetic Devices § 878.3925 Plastic surgery kit and accessories. (a) Identification. A plastic surgery kit and accessories is a device intended to...

  4. 21 CFR 878.3925 - Plastic surgery kit and accessories.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Plastic surgery kit and accessories. 878.3925... (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Prosthetic Devices § 878.3925 Plastic surgery kit and accessories. (a) Identification. A plastic surgery kit and accessories is a device intended to...

  5. 21 CFR 880.5740 - Suction snakebite kit.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Suction snakebite kit. 880.5740 Section 880.5740... Devices § 880.5740 Suction snakebite kit. (a) Identification. A suction snakebite kit is a device consisting of a knife, suction device, and tourniquet used for first-aid treatment of snakebites by removing...

  6. YourSELF. Middle School Nutrition Education Kit [Multimedia].

    ERIC Educational Resources Information Center

    Department of Agriculture, Washington, DC.

    This multimedia kit provides information and materials for teaching nutrition to middle school students (grades 7 and 8). The kit supports schools' efforts to make school meals healthier and more appealing to students. The materials provide information about the relationships between food, nutrition, growth, and health. The kit speaks directly to…

  7. 21 CFR 864.2260 - Chromosome culture kit.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Chromosome culture kit. 864.2260 Section 864.2260...) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Cell And Tissue Culture Products § 864.2260 Chromosome culture kit. (a) Identification. A chromosome culture kit is a device containing the necessary...

  8. 21 CFR 864.2260 - Chromosome culture kit.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Chromosome culture kit. 864.2260 Section 864.2260...) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Cell And Tissue Culture Products § 864.2260 Chromosome culture kit. (a) Identification. A chromosome culture kit is a device containing the necessary...

  9. 21 CFR 868.5140 - Anesthesia conduction kit.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Anesthesia conduction kit. 868.5140 Section 868...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Therapeutic Devices § 868.5140 Anesthesia conduction kit. (a) Identification. An anesthesia conduction kit is a device used to administer to a patient conduction, regional, or...

  10. 21 CFR 868.5140 - Anesthesia conduction kit.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Anesthesia conduction kit. 868.5140 Section 868...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Therapeutic Devices § 868.5140 Anesthesia conduction kit. (a) Identification. An anesthesia conduction kit is a device used to administer to a patient conduction, regional, or...

  11. 21 CFR 868.5140 - Anesthesia conduction kit.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Anesthesia conduction kit. 868.5140 Section 868...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Therapeutic Devices § 868.5140 Anesthesia conduction kit. (a) Identification. An anesthesia conduction kit is a device used to administer to a patient conduction, regional, or...

  12. 21 CFR 868.5140 - Anesthesia conduction kit.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Anesthesia conduction kit. 868.5140 Section 868...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Therapeutic Devices § 868.5140 Anesthesia conduction kit. (a) Identification. An anesthesia conduction kit is a device used to administer to a patient conduction, regional, or...

  13. 21 CFR 868.5140 - Anesthesia conduction kit.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Anesthesia conduction kit. 868.5140 Section 868...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Therapeutic Devices § 868.5140 Anesthesia conduction kit. (a) Identification. An anesthesia conduction kit is a device used to administer to a patient conduction, regional, or...

  14. Home Pregnancy Test Kits: How Readable Are the Instructions?

    ERIC Educational Resources Information Center

    Holcomb, Carol Ann

    At the conclusion of their study on home pregnancy test kits, Valinas and Perlman (1982) suggested that the instructions accompanying the kits be revised to make them easier to read. A study was undertaken to determine the readability of the printed instructions accompanying five home pregnancy test kits (Daisy II, Answer, Acu-Test, Predictor, and…

  15. 46 CFR 108.707 - First aid kit.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false First aid kit. 108.707 Section 108.707 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Miscellaneous Equipment § 108.707 First aid kit. Each unit must have a first-aid kit approved by the Mine...

  16. 46 CFR 108.707 - First aid kit.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false First aid kit. 108.707 Section 108.707 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Miscellaneous Equipment § 108.707 First aid kit. Each unit must have a first-aid kit approved by the Mine...

  17. Comparison of the purity and vitality of natural killer cells with different isolation kits.

    PubMed

    Wang, Guangchuan; Yu, Guang; Wang, Dongmei; Guo, Shengnan; Shan, Fengping

    2017-05-01

    Natural killer (NK) cells are innate lymphocytes that aid in the protection of the host from infectious diseases and cancer. In vitro studies of NK cells have provided a foundation for developing clinical adoptive NK-cell transferred immunotherapy against human tumors. To elucidate the functions and mechanisms of NK cell populations, it is important to develop an optimal, highly reproducible and reliable isolation method. The present comparative study was performed with four different NK cell isolation kits of magnetic bead labeling made by Miltenyi and Stemcell companies, including positive selection kits [cluster of differentiation (CD)-49b, using the monoclonal antibody DX5) MicroBeads] and negative selection kits. In addition, the viability of NK cells isinterleukin-2 (IL-2)-dependent in vitro and thus the concentration of IL-2 is critical for maintaining longer cell viability of NK cells. NK cell purity and viability after culturing, for 24, 48 or 72 h, with or without IL-2 (0, 100, 300 or 500 U/ml) was investigated in the present study. Purity of NK cells varied depending on the purification kit used, despite the same method being applied. Furthermore, more granulocytes were present in purified NK cells using Miltenyi sorting kits, particularly when using the negative selection kit. The main disadvantage of DX5-positive selection using the Stemcell and Miltenyi kits was that a high percentage of CD3ε(+) cells were mixed into the isolated NK cells. Additionally, a significant difference of NK cell purity (P=0.003) was observed while purification was performed using different surface markers. As a consequence, the use of the positive selection kit was modified and subsequently a significantly higher purity (P=0.002) and yield (P=0.004) of NK cells was obtained. Moreover, the purity of NK cells and viability with or without a range of concentrations of IL-2 was compared. Results indicated that with a higher IL-2 concentration, the NK cell purity and viability

  18. Preparation and characterization of electrodes for the NASA Redox storage system. [Iron and chromium chloride reactants

    SciTech Connect

    Reid, M.A.; Gahn, R.F.; Ling, J.S.; Charleston, J.

    1980-01-01

    The current status of electrodes for the NASA Redox energy storage system based on iron and chromium chloride reactants is discussed. Difficulties in making well-behaved electrodes for the chromium side of the flow cell have been largely overcome. The physical properties of several lots of felt were determined. Refined procedures were developed for evaluating electrode performance in lab-scale cells. Experimental procedures for evaluating electrodes by cyclic voltammetry are described which minimize the IR losses due to the high internal resistance in the felt (distributed resistance). Improved methods for preparing electrodes were discovered which reduced the coevolution of hydrogen at the chromium electrode and eliminated the drop in voltage on discharge occasionally seen with previous electrodes. Single cells of 0.33 ft/sup 2/ area with improved membranes and electrodes are operating at over 80 percent voltage efficiency and coulombic efficiencies of over 98 percent at current densities of 16 to 20 amp/ft/sup 2/.

  19. Power Reactant Storage Assembly (PRSA) (Space Shuttle). PRSA hydrogen and oxygen DVT tank refurbishment

    NASA Technical Reports Server (NTRS)

    1993-01-01

    The Power Reactant Storage Assembly (PRSA) liquid hydrogen Development Verification Test (H2 DVT) tank assembly (Beech Aircraft Corporation P/N 15548-0116-1, S/N 07399000SHT0001) and liquid oxygen (O2) DVT tank assembly (Beech Aircraft Corporation P/N 15548-0115-1, S/N 07399000SXT0001) were refurbished by Ball Electro-Optics and Cryogenics Division to provide NASA JSC, Propulsion and Power Division, the capability of performing engineering tests. The refurbishments incorporated the latest flight configuration hardware and avionics changes necessary to make the tanks function like flight articles. This final report summarizes these refurbishment activities. Also included are up-to-date records of the pressure time and cycle histories.

  20. Crosslinking-property relationships in PMR polyimide composites. I. [polymerization of monomer reactants

    NASA Technical Reports Server (NTRS)

    Pater, R. H.; Whitley, K.; Morgan, C.; Chang, A.

    1991-01-01

    The effect of the crosslink density of the matrix on physical and mechanical properties of a graphite-fiber-reinforced PMR (for polymerization of monomer reactants) polyimide composites during isothermal aging was investigated in experiments where unidirectional composite specimens of Celion 6000/PMR-P1 were isothermally exposed at 288 C in air for various time periods up to 5000 hrs. It was found that, as the crosslink density increased, the glass transition temperature, density, and elevated-temperature interlaminar shear strength of a composite increased, while the initial moisture absorption and the coefficient of thermal expansion decreased. However, after reaching the highest possible matrix crosslink density, several of the composite properties began to deteriorate rapidly.

  1. Power Reactant Storage Assembly (PRSA) (Space Shuttle). PRSA hydrogen and oxygen DVT tank refurbishment

    NASA Astrophysics Data System (ADS)

    1993-07-01

    The Power Reactant Storage Assembly (PRSA) liquid hydrogen Development Verification Test (H2 DVT) tank assembly (Beech Aircraft Corporation P/N 15548-0116-1, S/N 07399000SHT0001) and liquid oxygen (O2) DVT tank assembly (Beech Aircraft Corporation P/N 15548-0115-1, S/N 07399000SXT0001) were refurbished by Ball Electro-Optics and Cryogenics Division to provide NASA JSC, Propulsion and Power Division, the capability of performing engineering tests. The refurbishments incorporated the latest flight configuration hardware and avionics changes necessary to make the tanks function like flight articles. This final report summarizes these refurbishment activities. Also included are up-to-date records of the pressure time and cycle histories.

  2. Isochoric Burn, an Internally Consistent Method for the Reactant to Product Transformation in Reactive Flow

    SciTech Connect

    Reaugh, J E; Lee, E L

    2002-07-01

    Mixture rules for partially reacted explosives differ amongst various models. For instance, JWL++ uses a partial pressure addition to compute an average zonal pressure, Ignition and Growth requires pressure equilibration and thermal equilibration of temperature dependent JWL EOSs, CHEETAH In Line RF also assumes temperature and pressure equilibration. It has been suggested in the past that a more realistic equilibration scheme should comprise isentropic pressure equilibration of the separate reacted and unreacted phases. This turns out not to be a proper path for equilibration. Rather, we find that the only internally consistent method is the evaluation of the equilibrium pressure that satisfies the particular conditions of reactant and product resulting from deflagration in a fixed volume.

  3. Controlling an electron-transfer reaction at a metal surface by manipulating reactant motion and orientation.

    PubMed

    Bartels, Nils; Krüger, Bastian C; Auerbach, Daniel J; Wodtke, Alec M; Schäfer, Tim

    2014-12-08

    The loss or gain of vibrational energy in collisions of an NO molecule with the surface of a gold single crystal proceeds by electron transfer. With the advent of new optical pumping and orientation methods, we can now control all molecular degrees of freedom important to this electron-transfer-mediated process, providing the most detailed look yet into the inner workings of an electron-transfer reaction and showing how to control its outcome. We find the probability of electron transfer increases with increasing translational and vibrational energy as well as with proper orientation of the reactant. However, as the vibrational energy increases, translational excitation becomes unimportant and proper orientation becomes less critical. One can understand the interplay of all three control parameters from simple model potentials. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Validity of the Michaelis-Menten equation--steady-state or reactant stationary assumption: that is the question.

    PubMed

    Schnell, Santiago

    2014-01-01

    The Michaelis-Menten equation is generally used to estimate the kinetic parameters, V and K(M), when the steady-state assumption is valid. Following a brief overview of the derivation of the Michaelis-Menten equation for the single-enzyme, single-substrate reaction, a critical review of the criteria for validity of the steady-state assumption is presented. The application of the steady-state assumption makes the implicit assumption that there is an initial transient during which the substrate concentration remains approximately constant, equal to the initial substrate concentration, while the enzyme-substrate complex concentration builds up. This implicit assumption is known as the reactant stationary assumption. This review presents evidence showing that the reactant stationary assumption is distinct from and independent of the steady-state assumption. Contrary to the widely believed notion that the Michaelis-Menten equation can always be applied under the steady-state assumption, the reactant stationary assumption is truly the necessary condition for validity of the Michaelis-Menten equation to estimate kinetic parameters. Therefore, the application of the Michaelis-Menten equation only leads to accurate estimation of kinetic parameters when it is used under experimental conditions meeting the reactant stationary assumption. The criterion for validity of the reactant stationary assumption does not require the restrictive condition of choosing a substrate concentration that is much higher than the enzyme concentration in initial rate experiments.

  5. Kit Tests for Rapid Detection of Viable Bacteria and Viruses.

    DTIC Science & Technology

    1980-10-01

    Kit test Bacteria detection Kit bacteria Rapid detection Virus detection Viruses p-Iodonitrotetrazoiiu Viable bacteria Field kit Nucleic acid 21L...AD-A091 796 GEOMET TECHNOLOGIES INC GAITHERSBURG MD F/6 15/2 KIT TESTS FOR RAPID DETECTION OF VIABLE BACTERIA AND VIRUSES . IU) OCT V0 R N MOYER DAAKI...34 ®,,LEYE V TŘ CHEMICAL SYSTEMS LABORATORY CONTRACTOR REPORTo ARCSL-CR-80064 KIT TESTS FOR RAPID DETECTION OF VIABLE BACTERIA AND VIRUSES Final Report by R.H

  6. Effect of reactant solvent medium on structural and electrical properties of h-MoO{sub 3} nanocrystals

    SciTech Connect

    Veerathangam, K.; Chithambararaj, A.; Bose, A. Chandra

    2015-06-24

    The present work demonstrates the successful synthesis of hexagonal molybdenum oxide (h-MoO{sub 3}) nanocrystals by chemical precipitation method using two different reactant solvent medium such as water and ethanol. The influence of reactant solvent medium on structural, morphological and electrical properties is studied. X-ray diffraction (XRD) confirms the crystal phase of the materials and exhibits single phase h-MoO{sub 3}. Scanning electron microscope (SEM) images show that the particles are one dimensional hexagonal rods with different particle size distributions, influenced by the reactant solvent medium. The impedance results show grain and grain boundary conduction in h-MoO{sub 3}. The resultant grain and grain boundary resistance and capacitance values are measured by equivalent circuit model and the results are discussed.

  7. Sample rotating turntable kit for infrared spectrometers

    DOEpatents

    Eckels, Joel Del; Klunder, Gregory L.

    2008-03-04

    An infrared spectrometer sample rotating turntable kit has a rotatable sample cup containing the sample. The infrared spectrometer has an infrared spectrometer probe for analyzing the sample and the rotatable sample cup is adapted to receive the infrared spectrometer probe. A reflectance standard is located in the rotatable sample cup. A sleeve is positioned proximate the sample cup and adapted to receive the probe. A rotator rotates the rotatable sample cup. A battery is connected to the rotator.

  8. QCT and QM calculations of the Cl(2P) + NH3 reaction: influence of the reactant well on the dynamics.

    PubMed

    Monge-Palacios, M; Yang, M; Espinosa-García, J

    2012-04-14

    A detailed dynamics study, using both quasi-classical trajectory (QCT) and reduced-dimensional quantum mechanical (QM) calculations, was carried out to understand the reactivity and mechanism of the Cl((2)P) + NH(3)→ HCl + NH(2) gas-phase reaction, which evolves through deep wells in the entry and exit channels. The calculations were performed on an analytical potential energy surface recently developed by our group, PES-2010 [M. Monge-Palacios, C. Rangel, J. C. Corchado and J. Espinosa-Garcia, Int. J. Quantum. Chem., 2011], together with a simplified model surface, mod-PES, in which the reactant well is removed to analyze its influence. The main finding was that the QCT and QM methods show a change of the reaction probability with collision energy, suggesting a change of the atomic-level mechanism of reaction with energy. This change disappeared when the mod-PES was used, showing that the behaviour at low energies is a direct consequence of the existence of the reactant well. Analysis of the trajectories showed that different mechanisms operate depending on the collision energy. Thus, while at high energies (E(coll) > 5 kcal mol(-1)) practically all trajectories are direct, at low energies (E(coll) < 3 kcal mol(-1)) the trajectories are indirect, i.e., with the mediation of a trapping complex in the entry and/or the exit wells. The reactant complex allows repeated encounters between the reactants, increasing the reaction probability at low energies. The differential cross section results reinforce this change of mechanism, showing also the influence of the reactant well on this reaction. Thus, the PES-2010 surface yields a forward-backward symmetry in the scattering, while when the reactant well is removed with the mod-PES the shape is more isotropic.

  9. Shuttle orbiter TPS flight repair kit development

    NASA Technical Reports Server (NTRS)

    1979-01-01

    The design and application of a TPS repair kit is presented. The repair kit is designed for on orbit use by a crew member working in the manned maneuvering unit (MMU). The kit includes the necessary equipment and materials to accomplish the repair tasks which include the following: HRSI emittance coating repair, damaged tile repair, missing tile repair, and multiple tile repair. Two types of repair materials required to do the small area repair and the large area repair are described. The materials area cure in place, silicone base ablator for small damaged areas and precured ablator tile for repair of larger damaged areas is examined. The cure in place ablator is also used as an adhesive to bond the precured tiles in place. An applicator for the cure in place ablator, designed to contain a two-part silicon compound, mix the two components at correct ratio, and dispense the materials at rates compatible with mission timelines established for the EVA is described.

  10. A liquid-gas phase mixed-reactant fuel cell with a RuSeW cathode electrocatalyst

    NASA Astrophysics Data System (ADS)

    Cheng, H.; Yuan, W.; Scott, K.

    Some data in mixed-reactant fuel cells (MRFC) at Newcastle using formic acid, methanol and ethanol are reported. The importance of using a fuel-tolerant selective cathode catalyst has been identified. The influence of fuel and oxidant conditions and feeding patterns has been evaluated. The cell performance using air, oxygen and hydrogen peroxide is reported. The highest peak power density of 16 mW cm -2 was obtained with formic acid. The MRFC gave power densities approximately half those of a conventional, un-mixed-reactant fuel cell.

  11. The Insight ToolKit image registration framework

    PubMed Central

    Avants, Brian B.; Tustison, Nicholas J.; Stauffer, Michael; Song, Gang; Wu, Baohua; Gee, James C.

    2014-01-01

    Publicly available scientific resources help establish evaluation standards, provide a platform for teaching and improve reproducibility. Version 4 of the Insight ToolKit (ITK4) seeks to establish new standards in publicly available image registration methodology. ITK4 makes several advances in comparison to previous versions of ITK. ITK4 supports both multivariate images and objective functions; it also unifies high-dimensional (deformation field) and low-dimensional (affine) transformations with metrics that are reusable across transform types and with composite transforms that allow arbitrary series of geometric mappings to be chained together seamlessly. Metrics and optimizers take advantage of multi-core resources, when available. Furthermore, ITK4 reduces the parameter optimization burden via principled heuristics that automatically set scaling across disparate parameter types (rotations vs. translations). A related approach also constrains steps sizes for gradient-based optimizers. The result is that tuning for different metrics and/or image pairs is rarely necessary allowing the researcher to more easily focus on design/comparison of registration strategies. In total, the ITK4 contribution is intended as a structure to support reproducible research practices, will provide a more extensive foundation against which to evaluate new work in image registration and also enable application level programmers a broad suite of tools on which to build. Finally, we contextualize this work with a reference registration evaluation study with application to pediatric brain labeling.1 PMID:24817849

  12. A survival Kit for pancreatic beta cells: stem cell factor and c-Kit receptor tyrosine kinase.

    PubMed

    Feng, Zhi-Chao; Riopel, Matthew; Popell, Alex; Wang, Rennian

    2015-04-01

    The interactions between c-Kit and its ligand, stem cell factor (SCF), play an important role in haematopoiesis, pigmentation and gametogenesis. c-Kit is also found in the pancreas, and recent studies have revealed that c-Kit marks a subpopulation of highly proliferative pancreatic endocrine cells that may harbour islet precursors. c-Kit governs and maintains pancreatic endocrine cell maturation and function via multiple signalling pathways. In this review we address the importance of c-Kit signalling within the pancreas, including its profound role in islet morphogenesis, islet vascularisation, and beta cell survival and function. We also discuss the impact of c-Kit signalling in pancreatic disease and the use of c-Kit as a potential target for the development of cell-based and novel drug therapies in the treatment of diabetes.

  13. Surface chemistry of aromatic reactants on Pt- and Mo-modified Pt catalysts

    DOE PAGES

    Robinson, Allison M.; Mark, Lesli; Rasmussen, Mathew J.; ...

    2016-11-01

    Supported catalysts containing an oxophilic metal such as Mo and a noble metal such as Pt have shown promising activity and selectivity for deoxygenation of biomass-derived compounds. Here, we report that PtMo catalysts also promote hydrogenolysis of the model compound benzyl alcohol, while decarbonylation is most prevalent over unmodified Pt. A combination of single crystal surface science studies, density functional theory (DFT) calculations, and vapor phase upgrading experiments using supported catalysts was carried out to better understand the mechanism by which Mo promotes deoxygenation. Molybdenum was deposited in submonolayer quantities on a Pt(111) surface and reduced at high temperature. Temperature-programmedmore » desorption (TPD) experiments using benzyl alcohol as a reactant showed greatly enhanced yields of the deoxygenation product toluene at moderate Mo coverages. To understand how the interaction of the aromatic group with the surface influenced this reactivity, we investigated the adsorption of toluene as a probe molecule. We found that the addition of Mo to Pt(111) resulted in a significant decrease in toluene decomposition. DFT calculations indicated that this decrease was consistent with decreased aromatic adsorption strengths that accompany incorporation of Mo into the Pt subsurface. The weaker aromatic-surface interaction on Pt/Mo surfaces led to a tilted adsorption geometry for benzyl alcohol, which presumably promotes hydrogenolysis to produce toluene instead of decarbonylation to produce benzene and CO. Alumina-supported Pt and PtMo catalysts were also tested for benzyl alcohol deoxygenation. PtMo catalysts had a higher rate of toluene production and lower rates of benzene and benzaldehyde production. Additionally, when benzaldehyde was used as the reactant to measure decarbonylation activity the mass-normalized rate of benzene production was 2.5 times higher on Pt than PtMo. Altogether, the results of TPD, DFT, and supported catalyst

  14. Surface chemistry of aromatic reactants on Pt- and Mo-modified Pt catalysts

    SciTech Connect

    Robinson, Allison M.; Mark, Lesli; Rasmussen, Mathew J.; Hensley, Jesse E.; Medlin, J. Will

    2016-11-01

    Supported catalysts containing an oxophilic metal such as Mo and a noble metal such as Pt have shown promising activity and selectivity for deoxygenation of biomass-derived compounds. Here, we report that PtMo catalysts also promote hydrogenolysis of the model compound benzyl alcohol, while decarbonylation is most prevalent over unmodified Pt. A combination of single crystal surface science studies, density functional theory (DFT) calculations, and vapor phase upgrading experiments using supported catalysts was carried out to better understand the mechanism by which Mo promotes deoxygenation. Molybdenum was deposited in submonolayer quantities on a Pt(111) surface and reduced at high temperature. Temperature-programmed desorption (TPD) experiments using benzyl alcohol as a reactant showed greatly enhanced yields of the deoxygenation product toluene at moderate Mo coverages. To understand how the interaction of the aromatic group with the surface influenced this reactivity, we investigated the adsorption of toluene as a probe molecule. We found that the addition of Mo to Pt(111) resulted in a significant decrease in toluene decomposition. DFT calculations indicated that this decrease was consistent with decreased aromatic adsorption strengths that accompany incorporation of Mo into the Pt subsurface. The weaker aromatic-surface interaction on Pt/Mo surfaces led to a tilted adsorption geometry for benzyl alcohol, which presumably promotes hydrogenolysis to produce toluene instead of decarbonylation to produce benzene and CO. Alumina-supported Pt and PtMo catalysts were also tested for benzyl alcohol deoxygenation. PtMo catalysts had a higher rate of toluene production and lower rates of benzene and benzaldehyde production. Additionally, when benzaldehyde was used as the reactant to measure decarbonylation activity the mass-normalized rate of benzene production was 2.5 times higher on Pt than PtMo. Altogether, the results of TPD, DFT, and supported catalyst

  15. ISRU Reactant, Fuel Cell Based Power Plant for Robotic and Human Mobile Exploration Applications

    NASA Astrophysics Data System (ADS)

    Baird, Russell S.; Sanders, Gerald; Simon, Thomas; McCurdy, Kerri

    2003-01-01

    Three basic power generation system concepts are generally considered for lander, rover, and Extra-Vehicular Activity (EVA) assistant applications for robotic and human Moon and Mars exploration missions. The most common power system considered is the solar array and battery system. While relatively simple and successful, solar array/battery systems have some serious limitations for mobile applications. For typical rover applications, these limitations include relatively low total energy storage capabilities, daylight only operating times (6 to 8 hours on Mars), relatively short operating lives depending on the operating environment, and rover/lander size and surface use constraints. Radioisotope power systems are being reconsidered for long-range science missions. Unfortunately, the high cost, political controversy, and launch difficulties that are associated with nuclear-based power systems suggests that the use of radioisotope powered landers, rovers, and EVA assistants will be limited. The third power system concept now being considered are fuel cell based systems. Fuel cell power systems overcome many of the performance and surface exploration limitations of solar array/battery power systems and the prohibitive cost and other difficulties associated with nuclear power systems for mobile applications. In an effort to better understand the capabilities and limitations of fuel cell power systems for Moon and Mars exploration applications. NASA is investigating the use of In-Situ Resource Utilization (ISRU) produced reactant, fuel cell based power plants to power robotic outpost rovers, science equipment, and future human spacecraft, surface-excursion rovers, and EVA assistant rovers. This paper will briefly compare the capabilities and limitations of fuel cell power systems relative to solar array/battery and nuclear systems, discuss the unique and enhanced missions that fuel cell power systems enable, and discuss the common technology and system attributes

  16. ISRU Reactant, Fuel Cell Based Power Plant for Robotic and Human Mobile Exploration Applications

    NASA Technical Reports Server (NTRS)

    Baird, Russell S.; Sanders, Gerald; Simon, Thomas; McCurdy, Kerri

    2003-01-01

    Three basic power generation system concepts are generally considered for lander, rover, and Extra-Vehicular Activity (EVA) assistant applications for robotic and human Moon and Mars exploration missions. The most common power system considered is the solar array and battery system. While relatively simple and successful, solar array/battery systems have some serious limitations for mobile applications. For typical rover applications, these limitations include relatively low total energy storage capabilities, daylight only operating times (6 to 8 hours on Mars), relatively short operating lives depending on the operating environment, and rover/lander size and surface use constraints. Radioisotope power systems are being reconsidered for long-range science missions. Unfortunately, the high cost, political controversy, and launch difficulties that are associated with nuclear-based power systems suggests that the use of radioisotope powered landers, rovers, and EVA assistants will be limited. The third power system concept now being considered are fuel cell based systems. Fuel cell power systems overcome many of the performance and surface exploration limitations of solar array/battery power systems and the prohibitive cost and other difficulties associated with nuclear power systems for mobile applications. In an effort to better understand the capabilities and limitations of fuel cell power systems for Moon and Mars exploration applications, NASA is investigating the use of in-Situ Resource Utilization (ISRU) produced reactant, fuel cell based power plants to power robotic outpost rovers, science equipment, and future human spacecraft, surface-excursion rovers, and EVA assistant rovers. This paper will briefly compare the capabilities and limitations of fuel cell power systems relative to solar array/battery and nuclear systems, discuss the unique and enhanced missions that fuel cell power systems enable, and discuss the common technology and system attributes

  17. Optimising the diagnostic strategy for onychomycosis from sample collection to FUNGAL identification evaluation of a diagnostic kit for real-time PCR.

    PubMed

    Petinataud, Dimitri; Berger, Sibel; Ferdynus, Cyril; Debourgogne, Anne; Contet-Audonneau, Nelly; Machouart, Marie

    2016-05-01

    Onychomycosis is a common nail disorder mainly due to dermatophytes for which the conventional diagnosis requires direct microscopic observation and culture of a biological sample. Nevertheless, antifungal treatments are commonly prescribed without a mycological examination having been performed, partly because of the slow growth of dermatophytes. Therefore, molecular biology has been applied to this pathology, to support a quick and accurate distinction between onychomycosis and other nail damage. Commercial kits are now available from several companies for improving traditional microbiological diagnosis. In this paper, we present the first evaluation of the real-time PCR kit marketed by Bio Evolution for the diagnosis of dermatophytosis. Secondly, we compare the efficacy of the kit on optimal and non-optimal samples. This study was conducted on 180 nails samples, processed by conventional methods and retrospectively analysed using this kit. According to our results, this molecular kit has shown high specificity and sensitivity in detecting dermatophytes, regardless of sample quality. On the other hand, and as expected, optimal samples allowed the identification of a higher number of dermatophytes by conventional mycological diagnosis, compared to non-optimal samples. Finally, we have suggested several strategies for the practical use of such a kit in a medical laboratory for quick pathogen detection.

  18. Megakaryocytes compensate for Kit insufficiency in murine arthritis

    PubMed Central

    Cunin, Pierre; Penke, Loka R.; Thon, Jonathan N.; Monach, Paul A.; Jones, Tatiana; Chang, Margaret H.; Chen, Mary M.; Melki, Imene; Gurish, Michael F.; Italiano, Joseph E.; Boilard, Eric; Nigrovic, Peter A.

    2017-01-01

    The growth factor receptor Kit is involved in hematopoietic and nonhematopoietic development. Mice bearing Kit defects lack mast cells; however, strains bearing different Kit alleles exhibit diverse phenotypes. Herein, we investigated factors underlying differential sensitivity to IgG-mediated arthritis in 2 mast cell–deficient murine lines: KitWsh/Wsh, which develops robust arthritis, and KitW/Wv, which does not. Reciprocal bone marrow transplantation between KitW/Wv and KitWsh/Wsh mice revealed that arthritis resistance reflects a hematopoietic defect in addition to mast cell deficiency. In KitW/Wv mice, restoration of susceptibility to IgG-mediated arthritis was neutrophil independent but required IL-1 and the platelet/megakaryocyte markers NF-E2 and glycoprotein VI. In KitW/Wv mice, platelets were present in numbers similar to those in WT animals and functionally intact, and transfer of WT platelets did not restore arthritis susceptibility. These data implicated a platelet-independent role for the megakaryocyte, a Kit-dependent lineage that is selectively deficient in KitW/Wv mice. Megakaryocytes secreted IL-1 directly and as a component of circulating microparticles, which activated synovial fibroblasts in an IL-1–dependent manner. Transfer of WT but not IL-1–deficient megakaryocytes restored arthritis susceptibility to KitW/Wv mice. These findings identify functional redundancy among Kit-dependent hematopoietic lineages and establish an unanticipated capacity of megakaryocytes to mediate IL-1–driven systemic inflammatory disease. PMID:28375155

  19. Experimental and numerical analysis of parallel reactant flow and transverse mixing with mineral precipitation in homogeneous and heterogeneous porous media

    SciTech Connect

    Fox, Don T.; Guo, Luanjing; Fujita, Yoshiko; Huang, Hai; Redden, George

    2015-12-17

    Formation of mineral precipitates in the mixing interface between two reactant solutions flowing in parallel in porous media is governed by reactant mixing by diffusion and dispersion and is coupled to changes in porosity/permeability due to precipitation. The spatial and temporal distribution of mixing-dependent precipitation of barium sulfate in porous media was investigated with side-by-side injection of barium chloride and sodium sulfate solutions in thin rectangular flow cells packed with quartz sand. The results for homogeneous sand beds were compared to beds with higher or lower permeability inclusions positioned in the path of the mixing zone. In the homogeneous and high permeability inclusion experiments, BaSO4 precipitate (barite) formed in a narrow deposit along the length and in the center of the solution–solution mixing zone even though dispersion was enhanced within, and downstream of, the high permeability inclusion. In the low permeability inclusion experiment, the deflected BaSO4 precipitation zone broadened around one side and downstream of the inclusion and was observed to migrate laterally toward the sulfate solution. A continuum-scale fully coupled reactive transport model that simultaneously solves the nonlinear governing equations for fluid flow, transport of reactants and geochemical reactions was used to simulate the experiments and provide insight into mechanisms underlying the experimental observations. Lastly, migration of the precipitation zone in the low permeability inclusion experiment could be explained by the coupling effects among fluid flow, reactant transport and localized mineral precipitation reaction.

  20. Single cell performance studies on the FE/CR Redox Energy Storage System using mixed reactant solutions at elevated temperature

    NASA Technical Reports Server (NTRS)

    Gahn, R. F.; Hagedorn, N. H.; Ling, J. S.

    1983-01-01

    Experimental studies in a 14.5 sq cm single cell system using mixed reactant solutions at 65 C are described. Systems were tested under isothermal conditions, i.e., reactants and the cell were at the same temperature. Charging and discharging performance were evaluated by measuring watt-hour and coulombic efficiencies, voltage-current relationships, hydrogen evolution and membrane resistivity. Watt-hour efficiencies ranged from 86 percent at 43 ma/sq cm to 75 percent at 129 ma/sq cm with corresponding coulombic efficiencies of 92 percent and 97 percent, respectively. Hydrogen evolution was less than 1 percent of the charge coulumbic capacity during charge-discharge cycling. Bismuth amd bismuth-lead catalyzed chromium electrodes maintained reversible performance and low hydrogen evolution under normal and adverse cycling conditions. Reblending of the anode and cathode solutions was successfully demonstrated to compensate for osmotic volume changes. Improved performance was obtained with mixed reactant systems in comparison to the unmixed reactant systems. Previously announced in STAR as N83-25042

  1. The Double Solid Reactant Method for modeling the release of trace elements from dissolving solid phases: I. Outline and limitations

    NASA Astrophysics Data System (ADS)

    Accornero, Marina; Marini, Luigi

    2008-10-01

    A Double Solid Reactant Method was elaborated from a suggestion of Marini (Geological sequestration of carbon dioxide: Thermodynamics, kinetics, and reaction path modeling. Developments in Geochemistry, Elsevier, Amsterdam, 2007) to simulate the release of trace elements during the progressive dissolution of solid phases. The method is based on the definition, for each dissolving solid, of both an entity whose thermodynamic and kinetic properties are known (either a pure mineral or a solid mixture) and a special reactant, that is, a material of known stoichiometry and unknown thermodynamic and kinetic properties. The special reactant is utilised to take into account the concentrations of trace elements in the dissolving solid phase. In this communication, the influence of several trace elements on the Δ G f o, Δ G r o and log K of the minerals considered by Lelli et al. (Environ Geol, 2007) and Accornero and Marini (Geobasi, 2007a; Proceedings of IMWA symposium, Cagliari, 27 31 May 2007b) was evaluated assuming ideal mixing in the solid state. These effects were found to be negligible for albite and the leucite latitic glass, limited for muscovites and chlorites, and slightly more important for apatites. These influences become progressively higher with increasing concentration of trace elements in these minerals. Based on these deviations in thermodynamic parameters, special reactants should not include oxide components with molar fractions higher than 0.003.

  2. Preprocedural serum levels of acute-phase reactants and prognosis after percutaneous coronary intervention.

    PubMed

    Rahel, Braim M; Visseren, Frank L J; Suttorp, Maarten-Jan; Plokker, Thijs H W; Kelder, Johannes C; de Jongh, Bartelt M; Bouter, K Paul; Diepersloot, Rob J A

    2003-10-15

    In this study we evaluate the value of baseline concentrations of acute-phase reactants on prognosis after percutaneous coronary intervention (PCI). Blood samples were drawn immediately before PCI to measure baseline concentrations of C-reactive protein (CRP), interleukin-6 (IL-6), lipoprotein(a) (Lp(a)), and fibrinogen. Follow-up data were collected at 8 months. Repeat PCI, CABG, myocardial infarction, and death were recorded as major adverse clinical events (MACE). Furthermore the recurrence of angina pectoris was noted. The study included 600 consecutive patients after a successful PCI. Sixty-four percent of the patients were stented. The mean age was 61.6 years and 68.9% were male. CRP levels were significantly higher in patients who were to have repeat angina as compared with those who were not (P=0.0322). IL-6 levels were not correlated with angina or MACE. Lp(a) and fibrinogen concentrations were both significantly related to MACE (P=0.0337 and P=0.0253, respectively). Our study clearly supports the role of inflammation in restenosis after PCI as measured in statistically higher levels of Lp(a) and fibrinogen in patients with MACE and CRP in patients with repeat angina.

  3. THE INFLUENCE OF BENZENE AS A TRACE REACTANT IN TITAN AEROSOL ANALOGS

    SciTech Connect

    Trainer, Melissa G.; Sebree, Joshua A.; Heidi Yoon, Y.; Tolbert, Margaret A.

    2013-03-20

    Benzene has been detected in Titan's atmosphere by Cassini instruments, with concentrations ranging from sub-ppb in the stratosphere to ppm in the ionosphere. Sustained levels of benzene in the haze formation region could signify that it is an important reactant in the formation of Titan's organic aerosol. To date, there have not been laboratory investigations to assess the influence of benzene on aerosol properties. We report a laboratory study on the chemical composition of organic aerosol formed from C{sub 6}H{sub 6}/CH{sub 4}/N{sub 2} via far ultraviolet irradiation (120-200 nm). The compositional results are compared to those from aerosol generated by a more ''traditional Titan'' mixture of CH{sub 4}/N{sub 2}. Our results show that even a trace amount of C{sub 6}H{sub 6} (10 ppm) has significant impact on the chemical composition and production rates of organic aerosol. There are several pathways by which photolyzed benzene may react to form larger molecules, both with and without the presence of CH{sub 4}, but many of these reaction mechanisms are only beginning to be explored for the conditions at Titan. Continued work investigating the influence of benzene in aerosol growth will advance understanding of this previously unstudied reaction system.

  4. Melatonin: reducing molecular pathology and dysfunction due to free radicals and associated reactants.

    PubMed

    Reiter, Russel J; Tan, Duan-Xian; Allegra, Mario

    2002-04-01

    Endogenously produced metabolites of ground state oxygen are highly reactive and destructive to intracellular and extracellular molecules. The resulting damage, referred to as oxidative stress, leads to molecular and cellular dysfunction. The destruction of essential macromolecules by oxygen-based reactants is the basis of some diseases and is believed to be involved in the processes of aging. Free radical scavengers and antioxidants neutralize and/or metabolically remove reactive species from cells before they carry out their destructive activities. Melatonin is a highly ubiquitous direct free radical scavenger and indirect antioxidant. This brief report summarizes the interactions of melatonin with reactive species and identifies the resulting products. The paper also defines the melatonin antioxidant cascade wherein not only melatonin but at least one of the products, i.e., N(1)-acetyl-N(2)-formyl-5-methoxykynuramine, formed as a result of melatonin scavenging hydrogen peroxide is also a potent scavenger. The review summarizes the data which shows that melatonin is not only a pharmacologically useful free radical scavenger but that it functions in this capacity at physiological concentrations as well. Finally, this report identifies high oxidative stress situations in humans where melatonin has proven effective in reducing the severity of the disease state. In the last decade there have been hundreds of publications documenting melatonin's protective actions against a vast array of conditions, e.g., ischemia/reperfusion injury, toxin exposure, lipopolysaccharide exposure, etc., where free radical damage is a component of the condition.

  5. Charge sensitivity approach to mutual polarization of reactants: molecular mechanics perspective.

    PubMed

    Stachowicz, Anna; Rogalski, Marek; Korchowiec, Jacek

    2013-10-01

    Charge sensitivity analysis (CSA) in force-field atoms resolution was applied to describe the mutual polarization of reactants as well as charge-transfer (CT) effects. An inclusion complex of β-cyclodextrin with salicylic acid was used as a model system. Three CSA models were taken into account and verified on a Born-Oppenheimer molecular dynamics (BOMD) trajectory. The models differed in terms of the equilibrium conditions imposed on the system. It was demonstrated that mutual polarization is an important source of stabilization, in contrast to the results obtained from static charge calculations. The energy lowering induced by CT was small and comparable to the CT stabilization that occurs in hydrogen-bonded systems. All models correctly described the main topological features of the BOMD energy surface. CSA in force-field atoms resolution qualitatively reproduced the charge reorganization accompanying hydrogen-bond formation. It was shown that CSA parameters are very sensitive to the bond formation process, which suggests that they could be applied in reactive force fields as detectors of newly formed chemical bonds.

  6. Transport proteins and acute phase reactant proteins in children with sickle cell anemia.

    PubMed Central

    Warrier, R. P.; Kuvibidila, S.; Gordon, L.; Humbert, J.

    1994-01-01

    Transport proteins, acute-phase reactant proteins (APRP), hematology, and anthropometry were studied in 34 sickle cell disease (SCD) children (20 boys, 14 girls) and 27 controls without growth deficits (13 boys, 14 girls) [corrected]. The age range was 1/2 to 16 1/2 years. Weight deficits (< 80%) by Waterlow's classification were observed in 41% of SCD boys and 25% of SCD girls, and height deficits (< 90%) were observed in 25% SCD boys and 25% girls. Mean white blood cell counts were significantly higher (P < .001) and hematocrit and hemoglobin (Hb) lower (P < .005) in SCD children than in controls. Although both groups had similar mean levels of albumin, transferrin, and APRP, SCD children had significantly lower mean levels of retinol-binding protein (RBP) (P < .001) and retinol-prealbumin (P < .001). Retinol-binding protein levels were abnormal in 18 (53%) SCD children and in only 23% controls (chi 2 = 14.06; P < 0.005); transferrin levels were abnormal in 20% of SCD children and in none of the controls. Children with SC and SF Hb phenotype had normal mean levels of RBP, whereas those with S beta thal and SS phenotype had levels below normal. Growth-retarded children by weight and height had reduced mean levels of RBP and prealbumin compared with growth-normal SCD children. The implication of primary protein-energy malnutrition on growth retardation in SCD children is under study. PMID:7512147

  7. Oxidative Status and Acute Phase Reactants in Patients with Environmental Asbestos Exposure and Mesothelioma

    PubMed Central

    Sezgi, Cengizhan; Taylan, Mahsuk; Selimoglu Sen, Hadice; Evliyaoğlu, Osman; Kaya, Halide; Abakay, Ozlem; Abakay, Abdurrahman; Tanrıkulu, Abdullah Cetin; Senyiğit, Abdurrahman

    2014-01-01

    Background and Objectives. The aim of this study was to investigate inflammatory indicators and oxidative status in patients with asbestos exposure with and without mesothelioma and to compare results with data from healthy subjects. Methods. Eighty people with exposure to environmental asbestos and without any disease, 46 mesothelioma patients, and a control group of 50 people without exposure to environmental asbestos were enrolled in this prospective study. Serum total oxidant level (TOL), total antioxidant capacity (TAC), and oxidative stress index (OSI), CRP, transferrin, ceruloplasmin, α-1 antitrypsin, ferritin, and copper levels were measured. Results. Mesothelioma group exhibited higher TOL, OSI, α1-antitrypsin, ferritin and copper levels as compared to the other groups (P < 0.001, P = 0.007, P < 0.0001, P < 0.001, and P < 0.001, resp.). Transferrin was lower in the mesothelioma group than in the other two groups (P < 0.001). The asbestos group had higher TOL, TAC, α1-antitrypsin, and transferrin levels (P < 0.001, P < 0.001, P < 0.001, and P < 0.001, resp.), as well as lower OSI and ferritin levels as compared to the control group (P < 0.001 and P < 0.001). Conclusions. We believe that elevated acute phase reactants and oxidative stress markers (TOL and OSI) in the mesothelioma group can be used as predictive markers for the development of asbestos-related malignancy. PMID:24592197

  8. A continuous flow microfluidic calorimeter: 3-D numerical modeling with aqueous reactants

    PubMed Central

    Sen, Mehmet A.; Kowalski, Gregory J.; Fiering, Jason; Larson, Dale

    2015-01-01

    A computational analysis of the reacting flow field, species diffusion and heat transfer processes with thermal boundary layer effects in a microchannel reactor with a coflow configuration was performed. Two parallel adjacent streams of aqueous reactants flow along a wide, shallow, enclosed channel in contact with a substrate, which is affixed to a temperature controlled plate. The Fluent computational fluid dynamics package solved the Navier–Stokes, mass transport and energy equations. The energy model, including the enthalpy of reaction as a nonuniform heat source, was validated by calculating the energy balance at several control volumes in the microchannel. Analysis reveals that the temperature is nearly uniform across the channel thickness, in the direction normal to the substrate surface; hence, measurements made by sensors at or near the surface are representative of the average temperature. Additionally, modeling the channel with a glass substrate and a silicone cover shows that heat transfer is predominantly due to the glass substrate. Finally, using the numerical results, we suggest that a microcalorimeter could be based on this configuration, and that temperature sensors such as optical nanohole array sensors could have sufficient spatial resolution to determine enthalpy of reaction. PMID:25937678

  9. [Relationship between acute phase reactant proteins (APRP) and fatigue during post-operative convalescence].

    PubMed

    Córdova Martínez, A; Del Villar Sordo, V

    2002-09-01

    Surgery originates an inflammatory state which triggers an acute phase response (APR). In this study, variations in the acute phase reactant proteins (APRP) and their relationship with fatigue were assessed. A total of 58 candidate patients for elective abdominal surgery participated in this study. In the preoperative period (PRE) and in the post-operative period (9 days [9-P], 27 days [27-P] and 45 days [45-P]), variations in serum APRP and fatigue were analyzed. In the post-operative period (9-P), a significant decrease in PT, ALB, PAB, TRF, and an increase in CER were observed. In 27-P, significant decreases were observed in PAB and PBR, with increased PT and CER, and without significant variations in ALB ad TRF. In the 45-P day, a concomitant increase in PT and CER was observed. Dynamometric parameters also decreased significantly in the post-operative period (9-P). In conclusion, our results demonstrate the existence of changes in serum proteins and in APRP after an elective abdominal surgical intervention, which are associated with fatigue, evaluated both by subjective and objective (dynamometry) means and the use of a mathematical model that considers weight, proteins and APRP.

  10. Slow reactant-water exchange and high catalytic performance of water-tolerant Lewis acids.

    PubMed

    Koito, Yusuke; Nakajima, Kiyotaka; Kobayashi, Hisayoshi; Hasegawa, Ryota; Kitano, Masaaki; Hara, Michikazu

    2014-06-23

    (31)P nuclear magnetic resonance (NMR) spectroscopic measurement with trimethylphosphine oxide (TMPO) was applied to evaluate the Lewis acid catalysis of various metal triflates in water. The original (31)P NMR chemical shift and line width of TMPO is changed by the direct interaction of TMPO molecules with the Lewis acid sites of metal triflates. [Sc(OTf)3] and [In(OTf)3] had larger changes in (31)P chemical shift and line width by formation of the Lewis acid-TMPO complex than other metal triflates. It originates from the strong interaction between the Lewis acid and TMPO, which results in higher stability of [Sc(OTf)3TMPO] and [In(OTf)3TMPO] complexes than other metal triflate-TMPO complexes. The catalytic activities of [Sc(OTf)3] and [In(OTf)3] for Lewis acid-catalyzed reactions with carbonyl compounds in water were far superior to the other metal triflates, which indicates that the high stability of metal triflate-carbonyl compound complexes cause high catalytic performance for these reactions. Density functional theory (DFT) calculation suggests that low LUMO levels of [Sc(OTf)3] and [In(OTf)3] would be responsible for the formation of stable coordination intermediate with nucleophilic reactant in water.

  11. [Effects of balneotherapy on the reactants of acute inflammation phase in ankylosing spondylitis].

    PubMed

    2009-01-01

    Ankylosing spondylitis (AS) is a chronic inflammatory disease that affects sacroiliac joints, spinal column and peripheral joints. Beside medication therapy, physical and balneotherapy play an important role in its complex treatment. The aim of the research was to establish serum concentrations of C-reactive protein (CRP), alpha 1-acid glycoprotein (alpha 1-AGP), ceruloplasmine (CP) and erythrocyte sedimentation rate (SE) before and after the balneotherapy in ankylosing spondylitis. The research included 50 AS patients according to the revised New York criteria, of mean age 43 years, who were treated for 14 days on the average at the Clinic for Rheumatology of the Institute "Niska Banja". All the patients received medications and balneotherapy (radioactive oligomineral baths, peloid, massage, kinesitherapy); the serum concentrations of CRP, al-AGP, CP and SE were measured before and after balneotherapy. Serum proteins were determined using original Nor Partigen plates Boehringer. Erythrocyte sedimentation rate was measured by Westergreen method. Balneotherapy was applied individually, intensively or mildly, depending on the AS stage and activity phase. After dosed balneotherapy, a significant decrease in the concentrations of CP (p < 0.05), alpha1-AGP (p < 0.01) and CRP (p < 0.05) was registered in the serums of AS patients. ESR was not significantly reduced. The research proved that alpha 1-acid glycoprotein, ceruloplasmine and C-reactive protein represent more sensitive inflammation markers as compared to erythrocyte sedimentation rate. The identification of acute phase reactants is important in the evaluation of dosed balneotherapy efficiency in the treatment of ankylosing spondylitis.

  12. A continuous flow microfluidic calorimeter: 3-D numerical modeling with aqueous reactants.

    PubMed

    Sen, Mehmet A; Kowalski, Gregory J; Fiering, Jason; Larson, Dale

    2015-03-10

    A computational analysis of the reacting flow field, species diffusion and heat transfer processes with thermal boundary layer effects in a microchannel reactor with a coflow configuration was performed. Two parallel adjacent streams of aqueous reactants flow along a wide, shallow, enclosed channel in contact with a substrate, which is affixed to a temperature controlled plate. The Fluent computational fluid dynamics package solved the Navier-Stokes, mass transport and energy equations. The energy model, including the enthalpy of reaction as a nonuniform heat source, was validated by calculating the energy balance at several control volumes in the microchannel. Analysis reveals that the temperature is nearly uniform across the channel thickness, in the direction normal to the substrate surface; hence, measurements made by sensors at or near the surface are representative of the average temperature. Additionally, modeling the channel with a glass substrate and a silicone cover shows that heat transfer is predominantly due to the glass substrate. Finally, using the numerical results, we suggest that a microcalorimeter could be based on this configuration, and that temperature sensors such as optical nanohole array sensors could have sufficient spatial resolution to determine enthalpy of reaction.

  13. Optimizing medical resources for spaceflight using the integrated medical model.

    PubMed

    Minard, Charles G; de Carvalho, Mary Freire; Iyengar, M Sriram

    2011-09-01

    Efficient allocation of medical resources for spaceflight is important for crew health. The Integrated Medical Model (IMM) was developed to estimate medical event occurrences, mitigation, and resource requirements. An optimization module was created for IMM that uses a systematic process of elimination and preservation to maximize crew health outcomes subject to resource constraints. A maximum medical kit is identified and resources are eliminated according to their relative impact on outcomes of interest. Additional steps allow opportunities for resources to be added back into the medical kit if possible. The effectiveness of the module is demonstrated under six alternative mission profiles by optimizing the medical kit to maximize the expected Crew Health Index (CHI), and comparisons are made with minimum and maximum kits. The optimum and maximum kits had similar expected CHI, but CHI was more variable for the optimum kit. The maximum kit resulted in the best outcomes, but required at least 13.7 times the mass of the optimum kit and 26.6 times the volume. The largest difference in mean CHI between the optimum and maximum kits occurred for four crewmembers on a 180-d mission (91.1% vs. 95.4%). The optimization module may be used as an objective tool to assist with the efficient allocation of medical resources for spaceflight. The module provides a flexible algorithm that may be used in conjunction with the IMM model to assist in medical kit requirements and design.

  14. Food kit used by Mercury astronauts

    NASA Technical Reports Server (NTRS)

    1962-01-01

    Food kit used by Mercury astronauts. Some is dehydrated and needs water, other packets are ready to eat. Size is measured relative to a ruler. Included are packets of mushroom soup, orange-grapefruit juice, cocoa beverage, pineapple juice, chicken with gravy, pears, strawberries, beef and vegetables and other assorted food containers (08742-3); mechanism for connecting water dispensor to dehydrated food containers to facilitate rehydration (08744); Group packets of ready to eat space food, with size being measured by a ruler (8745).

  15. The Fluid Dynamics Demo Kit: Part II

    NASA Astrophysics Data System (ADS)

    Underhill, Patrick; Fix, Hannah; Haines, Timothy; Prestridge, Kathy; Flack, Karen

    2012-11-01

    This talk will focus on the current contents and efforts building a fluid dynamics demonstration/experiment kit through the APS-DFD Mentoring and Outreach Committee and with funding from the APS DFD. The current experiments include predicting how far the water from a water gun will go, predicting how long a Heron's fountain will run, measuring the viscosity of corn syrup by measuring terminal velocity, and measuring the flow rate through a siphon. We will discuss the testing and development of these experiments and the results of field testing. Ideas for future experiments will also be discussed.

  16. Food kit used by Mercury astronauts

    NASA Technical Reports Server (NTRS)

    1962-01-01

    Food kit used by Mercury astronauts. Some is dehydrated and needs water, other packets are ready to eat. Size is measured relative to a ruler. Included are packets of mushroom soup, orange-grapefruit juice, cocoa beverage, pineapple juice, chicken with gravy, pears, strawberries, beef and vegetables and other assorted food containers (08742-3); mechanism for connecting water dispensor to dehydrated food containers to facilitate rehydration (08744); Group packets of ready to eat space food, with size being measured by a ruler (8745).

  17. Raman monitoring of a catalytic system at work: Influence of the reactant on the sensitivity to laser-induced heating

    NASA Astrophysics Data System (ADS)

    Schnee, Josefine; Gaigneaux, Eric M.

    2017-02-01

    Characterizing catalysts under working conditions is crucial to understand and to optimize their behavior and performance. However, when Raman spectroscopy is used, attention has to be paid to laser-induced artefacts. While laser irradiation is often claimed to lead to a temperature gradient between the integral catalyst bed and the sampling point, neither the circumstances when such effect appears, nor if it systematically occurs or not, are really explored in details. The present paper shows that the sensitivity of a catalyst to laser-induced heating largely depends on the gas composition under which the analysis is done, in particular that it depends whether the catalyst has adsorbed reactant molecules or not. These aspects are here addressed via the Raman in situ exploration of H3PW12O40. This heteropolyacid is a widely used acid catalyst due to its very high Brönsted acidity, approaching the superacid region. In particular, we have investigated the impact of laser irradiation in the Raman monitoring of solid H3PW12O40 at work under a flow of methanol in nitrogen at 50 °C. When 1 single spectrum of H3PW12O40 was measured after 3 h of exposure to methanol, the characteristic Csbnd H vibration bands of adsorbed methanol appeared. However, when spectra were measured continuously throughout the experiment, the same Csbnd H vibration bands were observed only during the first hour, then they disappeared and the characteristic bands of polyaromatic molecules appeared. Under continuous laser irradiation, adsorbed methanol was thus converted into polyaromatic coke as resulting from a laser-induced heating. However, the spectra collected under pure nitrogen show that the laser does not heat the catalyst in the absence of methanol. UV-Vis revealed the reason of the laser-induced heating in the presence of methanol, and the subsequent formation of coke. Actually the catalyst gets reduced by the adsorbed methanol, what darkens the catalyst bed. Such a darkening renders

  18. Raman monitoring of a catalytic system at work: Influence of the reactant on the sensitivity to laser-induced heating.

    PubMed

    Schnee, Josefine; Gaigneaux, Eric M

    2017-02-15

    Characterizing catalysts under working conditions is crucial to understand and to optimize their behavior and performance. However, when Raman spectroscopy is used, attention has to be paid to laser-induced artefacts. While laser irradiation is often claimed to lead to a temperature gradient between the integral catalyst bed and the sampling point, neither the circumstances when such effect appears, nor if it systematically occurs or not, are really explored in details. The present paper shows that the sensitivity of a catalyst to laser-induced heating largely depends on the gas composition under which the analysis is done, in particular that it depends whether the catalyst has adsorbed reactant molecules or not. These aspects are here addressed via the Raman in situ exploration of H3PW12O40. This heteropolyacid is a widely used acid catalyst due to its very high Brönsted acidity, approaching the superacid region. In particular, we have investigated the impact of laser irradiation in the Raman monitoring of solid H3PW12O40 at work under a flow of methanol in nitrogen at 50°C. When 1 single spectrum of H3PW12O40 was measured after 3h of exposure to methanol, the characteristic CH vibration bands of adsorbed methanol appeared. However, when spectra were measured continuously throughout the experiment, the same CH vibration bands were observed only during the first hour, then they disappeared and the characteristic bands of polyaromatic molecules appeared. Under continuous laser irradiation, adsorbed methanol was thus converted into polyaromatic coke as resulting from a laser-induced heating. However, the spectra collected under pure nitrogen show that the laser does not heat the catalyst in the absence of methanol. UV-Vis revealed the reason of the laser-induced heating in the presence of methanol, and the subsequent formation of coke. Actually the catalyst gets reduced by the adsorbed methanol, what darkens the catalyst bed. Such a darkening renders the catalyst

  19. Comparison of presumptive blood test kits including hexagon OBTI.

    PubMed

    Johnston, Emma; Ames, Carole E; Dagnall, Kathryn E; Foster, John; Daniel, Barbara E

    2008-05-01

    Four presumptive blood tests, Hexagon OBTI, Hemastix(R), Leucomalachite green (LMG), and Kastle-Meyer (KM) were compared for their sensitivity in the identification of dried bloodstains. Stains of varying blood dilutions were subjected to each presumptive test and the results compared. The Hexagon OBTI buffer volume was also reduced to ascertain whether this increased the sensitivity of the kit. The study found that Hemastix(R) was the most sensitive test for trace blood detection. Only with the reduced buffer volume was the Hexagon OBTI kit as sensitive as the LMG and KM tests. However, the Hexagon OBTI kit has the advantage of being a primate specific blood detection kit. This study also investigated whether the OBTI buffer within the kit could be utilized for DNA profiling after presumptive testing. The results show that DNA profiles can be obtained from the Hexagon OBTI kit buffer directly.

  20. Quadruple wild-type (WT) GIST: defining the subset of GIST that lacks abnormalities of KIT, PDGFRA, SDH, or RAS signaling pathways.

    PubMed

    Pantaleo, Maria A; Nannini, Margherita; Corless, Christopher L; Heinrich, Michael C

    2015-01-01

    A subset of GISTs lack mutations in the KIT/PDGFRA or RAS pathways and yet retain an intact succinate dehydrogensase (SDH) complex. We propose that these KIT/PDGFRA/SDH/RAS-P WT GIST tumors be designated as quadruple wild-type (WT) GIST. Further molecular and clinicophatological characterization of quadruple WT GIST will help to determine their prognosis as well as assist in the optimization of medical management, including clinical test of novel therapies.

  1. To Kit or Not to Kit? Evaluating and Implementing Science Materials and Resources

    ERIC Educational Resources Information Center

    Schiller, Ellen; Melin, Jacque; Bair, Mary

    2016-01-01

    With the release of the "Next Generation Science Standards," many schools are reexamining the science materials they are using. Textbook companies and kit developers are eager to meet the demand for "NGSS"-aligned teaching materials. Teacher may have been asked to serve on a science curriculum committee, or to evaluate current…

  2. Selling Addiction: A Workshop Kit on Tobacco and Alcohol Advertising. A Media Literacy Workshop Kit.

    ERIC Educational Resources Information Center

    Barnes, Mary Ellen; And Others

    This kit consists of: (1) a leader's guide; (2) an 18-minute videotape containing three 6-minute discussion starter segments analyzing typical commercials and advertising techniques; (3) a special issue of "Media Values" magazine on the theme "Fatal Attraction: The Selling of Addiction"; (4) an 8-page booklet "Awareness to Action: Media Literacy…

  3. Selling Addiction: A Workshop Kit on Tobacco and Alcohol Advertising. A Media Literacy Workshop Kit.

    ERIC Educational Resources Information Center

    Barnes, Mary Ellen; And Others

    This kit consists of: (1) a leader's guide; (2) an 18-minute videotape containing three 6-minute discussion starter segments analyzing typical commercials and advertising techniques; (3) a special issue of "Media Values" magazine on the theme "Fatal Attraction: The Selling of Addiction"; (4) an 8-page booklet "Awareness to Action: Media Literacy…

  4. To Kit or Not to Kit? Evaluating and Implementing Science Materials and Resources

    ERIC Educational Resources Information Center

    Schiller, Ellen; Melin, Jacque; Bair, Mary

    2016-01-01

    With the release of the "Next Generation Science Standards," many schools are reexamining the science materials they are using. Textbook companies and kit developers are eager to meet the demand for "NGSS"-aligned teaching materials. Teacher may have been asked to serve on a science curriculum committee, or to evaluate current…

  5. Android: Call C Functions with the Native Development Kit (NDK)

    DTIC Science & Technology

    2016-09-01

    ARL-TN-0782 ● SEP 2016 US Army Research Laboratory Android: Call C Functions with the Native Development Kit (NDK) by Hao Q...from a Java application. 15. SUBJECT TERMS Android, NDK, Native Development Kit , C callable, Java Native Interface, JNI, Java, C / C ++ 16. SECURITY...Development Kit (NDK) and the Java Native Interface (JNI) provides such a mechanism. NDK is a toolset that allows users to build C / C ++ functions

  6. Data Center Energy Efficiency Measurement Assessment Kit Guide and Specification

    SciTech Connect

    2012-10-26

    A portable and temporary wireless mesh assessment kit can be used to speed up and reduce the costs of a data center energy use assessment and overcome the issues with respect to shutdowns. The assessment kit is comprised of temperature, relative humidity, and pressure sensors. Also included are power meters that can be installed on computer room air conditioners (CRACs) without intrusive interruption of data center operations. The assessment kit produces data required for a detailed energy assessment of the data center.

  7. FUELS IN SOIL TEST KIT: FIELD USE OF DIESEL DOG SOIL TEST KITS

    SciTech Connect

    Susan S. Sorini; John F. Schabron; Joseph F. Rovani, Jr.

    2002-09-30

    Western Research Institute (WRI) has developed a new commercial product ready for technology transfer, the Diesel Dog{reg_sign} Portable Soil Test Kit, for performing analysis of fuel-contaminated soils in the field. The technology consists of a method developed by WRI (U.S. Patents 5,561,065 and 5,976,883) and hardware developed by WRI that allows the method to be performed in the field (patent pending). The method is very simple and does not require the use of highly toxic reagents. The aromatic components in a soil extract are measured by absorption at 254 nm with a field-portable photometer. WRI added significant value to the technology by taking the method through the American Society for Testing and Materials (ASTM) approval and validation processes. The method is designated as ASTM Method D 5831-96, Standard Test Method for Screening Fuels in Soils. This ASTM designation allows the method to be used for federal compliance activities. In June 2001, the Diesel Dog technology won an American Chemical Society Regional Industrial Innovations Award. To gain field experience with the new technology, Diesel Dog kits have been used for a variety of site evaluation and cleanup activities. Information gained from these activities has led to improvements in hardware configurations and additional insight into correlating Diesel Dog results with results from laboratory methods. The Wyoming Department of Environmental Quality (DEQ) used Diesel Dog Soil Test Kits to guide cleanups at a variety of sites throughout the state. ENSR, of Acton, Massachusetts, used a Diesel Dog Portable Soil Test Kit to evaluate sites in the Virgin Islands and Georgia. ChemTrack and the U.S. Army Corps of Engineers successfully used a test kit to guide excavation at an abandoned FAA fuel-contaminated site near Fairbanks, Alaska. Barenco, Inc. is using a Diesel Dog Portable Soil Test Kit for site evaluations in Canada. A small spill of diesel fuel was cleaned up in Laramie, Wyoming using a Diesel

  8. Does the presence of bacteria effect basaltic glass dissolution rates? 1: Dead Pseudomonas reactants

    NASA Astrophysics Data System (ADS)

    Stockmann, Gabrielle J.; Shirokova, Liudmila S.; Pokrovsky, Oleg S.; Oelkers, Eric H.; Benezeth, Pascale

    2010-05-01

    Basaltic glass and crystalline basalt formations in Iceland have been suggested for industrial CO2 storage due to their porous and permeable properties and high reactivity. Acid CO2-saturated waters in contact with basaltic glass will lead to rapid dissolution of the glass and release of divalent cations, (Ca2+, Mg2+, Fe2+) that can react to form stable carbonates and thereby trap the CO2. However, the basalt formations in Iceland not only contains glass and mineral assemblages, but also host microbiological communities that either by their presence or by active involvement in chemical reactions could affect the amount of basaltic glass being dissolved and CO2 being trapped. Samples of natural bacteria communities from the CO2 storage grounds in Iceland were collected, separated, and purified using agar plate technique and cultured under laboratory conditions in nutrient broth-rich media. Heterotrophic aerobic Gram-negative strain of Pseudomonas reactants was selected for a series of flow-through experiments aimed at evaluation of basaltic glass dissolution rate in the presense of increasing amounts of dead bacteria and their lysis products. The experiments were carried out using mixed-flow reactors at pH 4, 6, 8 and 10 at 25 °C. Each of the four reactors contained 1 gram of basaltic glass of the size fraction 45-125 μm. This glass was dissolved in ~ 0.01 M buffer solutions (acetate, MES, bicarbonate and carbonate+bicarbonate mixture) of the desired pH. All experiments ran 2 months, keeping the flowrate and temperature stable and only changing the concentration of dead bacteria in the inlet solutions (from 0 to 430 mg/L). Experiments were performed in sterile conditions, and bacterial growth was prevented by adding NaN3 to the inlet solutions. Routine culturing of bacteria on the agar plates confirmed the sterility of experiments. Samples of outlet solutions were analyzed for major cations and trace elements by ICP-MS. Results demonstrate a slight decrease in the

  9. The Virtual Physiological Human ToolKit.

    PubMed

    Cooper, Jonathan; Cervenansky, Frederic; De Fabritiis, Gianni; Fenner, John; Friboulet, Denis; Giorgino, Toni; Manos, Steven; Martelli, Yves; Villà-Freixa, Jordi; Zasada, Stefan; Lloyd, Sharon; McCormack, Keith; Coveney, Peter V

    2010-08-28

    The Virtual Physiological Human (VPH) is a major European e-Science initiative intended to support the development of patient-specific computer models and their application in personalized and predictive healthcare. The VPH Network of Excellence (VPH-NoE) project is tasked with facilitating interaction between the various VPH projects and addressing issues of common concern. A key deliverable is the 'VPH ToolKit'--a collection of tools, methodologies and services to support and enable VPH research, integrating and extending existing work across Europe towards greater interoperability and sustainability. Owing to the diverse nature of the field, a single monolithic 'toolkit' is incapable of addressing the needs of the VPH. Rather, the VPH ToolKit should be considered more as a 'toolbox' of relevant technologies, interacting around a common set of standards. The latter apply to the information used by tools, including any data and the VPH models themselves, and also to the naming and categorizing of entities and concepts involved. Furthermore, the technologies and methodologies available need to be widely disseminated, and relevant tools and services easily found by researchers. The VPH-NoE has thus created an online resource for the VPH community to meet this need. It consists of a database of tools, methods and services for VPH research, with a Web front-end. This has facilities for searching the database, for adding or updating entries, and for providing user feedback on entries. Anyone is welcome to contribute.

  10. Phase II Study of Nilotinib in Melanoma Harboring KIT Alterations Following Progression to Prior KIT Inhibition

    PubMed Central

    Carvajal, Richard D.; Lawrence, Donald P.; Weber, Jeffrey S.; Gajewski, Thomas F.; Gonzalez, Rene; Lutzky, Jose; O’Day, Steven J.; Hamid, Omid; Wolchok, Jedd D.; Chapman, Paul B.; Sullivan, Ryan J.; Teitcher, Jerrold B.; Ramaiya, Nikhil; Giobbie-Hurder, Anita; Antonescu, Cristina R.; Heinrich, Michael C.; Bastian, Boris C.; Corless, Christopher L.; Fletcher, Jonathan A.; Hodi, F. Stephen

    2016-01-01

    Purpose Although durable responses can be achieved with tyrosine kinase inhibitors such as imatinib in melanomas harboring KIT mutations, the efficacy of alternative inhibitors after progression to imatinib and the activity of these agents on brain metastases is unknown. Experimental Design We conducted a phase II study of nilotinib 400 mg BID in two cohorts of patients with melanomas harboring KIT mutations or amplification: A) those refractory or intolerant to a prior KIT inhibitor; and B) those with brain metastases. The primary endpoint was 4-month disease control rate. Secondary endpoints included response rate, time-to-progression and overall survival. A Simon two-stage and a single-stage design was planned to assess for the primary endpoint in Cohorts A and B, respectively. Results Twenty patients were enrolled and 19 treated (11-Cohort A; 8-Cohort B). Three patients on Cohort A (27%; 95% CI, 8% – 56%) and 1 on Cohort B (12.5%; 90% CI, 0.6% – 47%) achieved the primary endpoint. Two partial responses were observed in Cohort A (18.2%, 90% CI, 3% – 47%); none were observed in Cohort B. The median time-to-progression and overall survival was 3·3 (90% CI, 2.1 – 3.9 months) and 9.1 months (90% CI, 4.3 – 14.2 months), respectively, in all treated patients. Conclusion Nilotinib may achieve disease control in patients with melanoma harboring KIT alterations and whose disease progressed after imatinib therapy. The efficacy of this agent in KIT altered melanoma with brain metastasis is limited. PMID:25695690

  11. Phase II Study of Nilotinib in Melanoma Harboring KIT Alterations Following Progression to Prior KIT Inhibition.

    PubMed

    Carvajal, Richard D; Lawrence, Donald P; Weber, Jeffrey S; Gajewski, Thomas F; Gonzalez, Rene; Lutzky, Jose; O'Day, Steven J; Hamid, Omid; Wolchok, Jedd D; Chapman, Paul B; Sullivan, Ryan J; Teitcher, Jerrold B; Ramaiya, Nikhil; Giobbie-Hurder, Anita; Antonescu, Cristina R; Heinrich, Michael C; Bastian, Boris C; Corless, Christopher L; Fletcher, Jonathan A; Hodi, F Stephen

    2015-05-15

    Although durable responses can be achieved with tyrosine kinase inhibitors such as imatinib in melanomas harboring KIT mutations, the efficacy of alternative inhibitors after progression to imatinib and the activity of these agents on brain metastases are unknown. We conducted a phase II study of nilotinib 400 mg twice a day in two cohorts of patients with melanomas harboring KIT mutations or amplification: (A) those refractory or intolerant to a prior KIT inhibitor; and (B) those with brain metastases. The primary endpoint was 4-month disease control rate. Secondary endpoints included response rate, time-to-progression (TTP), and overall survival (OS). A Simon two-stage and a single-stage design was planned to assess for the primary endpoint in cohorts A and B, respectively. Twenty patients were enrolled and 19 treated (11 in cohort A; 8 in cohort B). Three patients on cohort A [27%; 95% confidence interval (CI), 8%-56%] and 1 on cohort B (12.5%; 90% CI, 0.6%-47%) achieved the primary endpoint. Two partial responses were observed in cohort A (18.2%; 90% CI, 3%-47%); none were observed in cohort B. The median TTP and OS was 3.3 (90% CI, 2.1-3.9 months) and 9.1 months (90% CI, 4.3-14.2 months), respectively, in all treated patients. Nilotinib may achieve disease control in patients with melanoma harboring KIT alterations and whose disease progressed after imatinib therapy. The efficacy of this agent in KIT-altered melanoma with brain metastasis is limited. ©2015 American Association for Cancer Research.

  12. c-Kit signaling determines neointimal hyperplasia in arteriovenous fistulae.

    PubMed

    Skartsis, Nikolaos; Martinez, Laisel; Duque, Juan Camilo; Tabbara, Marwan; Velazquez, Omaida C; Asif, Arif; Andreopoulos, Fotios; Salman, Loay H; Vazquez-Padron, Roberto I

    2014-11-01

    Stenosis of arteriovenous (A-V) fistulae secondary to neointimal hyperplasia (NIH) compromises dialysis delivery, which worsens patients' quality of life and increases medical costs associated with the maintenance of vascular accesses. In the present study, we evaluated the role of the receptor tyrosine kinase c-Kit in A-V fistula neointima formation. Initially, c-Kit was found in the neointima and adventitia of human brachiobasilic fistulae, whereas it was barely detectable in control veins harvested at the time of access creation. Using the rat A-V fistula model to study venous vascular remodeling, we analyzed the spatial and temporal pattern of c-Kit expression in the fistula wall. Interestingly, c-Kit immunoreactivity increased with time after anastomosis, which concurred with the accumulation of cells in the venous intima. In addition, c-Kit expression in A-V fistulae was positively altered by chronic kidney failure conditions. Both blockade of c-Kit with imatinib mesylate (Gleevec) and inhibition of stem cell factor production with a specific short hairpin RNA prevented NIH in the outflow vein of experimental fistulae. In agreement with these data, impaired c-Kit activity compromised the development of NIH in A-V fistulae created in c-KitW/Wv mutant mice. These results suggest that targeting of the c-Kit signaling pathway may be an effective approach to prevent postoperative NIH in A-V fistulae.

  13. c-Kit signaling determines neointimal hyperplasia in arteriovenous fistulae

    PubMed Central

    Skartsis, Nikolaos; Martinez, Laisel; Duque, Juan Camilo; Tabbara, Marwan; Velazquez, Omaida C.; Asif, Arif; Andreopoulos, Fotios; Salman, Loay H.

    2014-01-01

    Stenosis of arteriovenous (A-V) fistulae secondary to neointimal hyperplasia (NIH) compromises dialysis delivery, which worsens patients' quality of life and increases medical costs associated with the maintenance of vascular accesses. In the present study, we evaluated the role of the receptor tyrosine kinase c-Kit in A-V fistula neointima formation. Initially, c-Kit was found in the neointima and adventitia of human brachiobasilic fistulae, whereas it was barely detectable in control veins harvested at the time of access creation. Using the rat A-V fistula model to study venous vascular remodeling, we analyzed the spatial and temporal pattern of c-Kit expression in the fistula wall. Interestingly, c-Kit immunoreactivity increased with time after anastomosis, which concurred with the accumulation of cells in the venous intima. In addition, c-Kit expression in A-V fistulae was positively altered by chronic kidney failure conditions. Both blockade of c-Kit with imatinib mesylate (Gleevec) and inhibition of stem cell factor production with a specific short hairpin RNA prevented NIH in the outflow vein of experimental fistulae. In agreement with these data, impaired c-Kit activity compromised the development of NIH in A-V fistulae created in c-KitW/Wv mutant mice. These results suggest that targeting of the c-Kit signaling pathway may be an effective approach to prevent postoperative NIH in A-V fistulae. PMID:25186298

  14. The effect of the reactant internal excitation on the dynamics of the C(+) + H2 reaction.

    PubMed

    Herráez-Aguilar, D; Jambrina, P G; Menéndez, M; Aldegunde, J; Warmbier, R; Aoiz, F J

    2014-12-07

    We have performed a dynamical study of the endothermic and barrierless C(+) + H2((1)Σg(+)) → CH(+)((1)Σg(+)) + H reaction for different initial rotational states of the H2(v = 0) and H2(v = 1) manifolds. The calculations have been carried out using quasiclassical trajectories and the Gaussian binning methodology on a recent potential energy surface [R. Warmbier and R. Schneider, Phys. Chem. Chem. Phys., 2011, 13, 10285]. Both state-selected integral cross sections as a function of the collision energy and rate coefficients, kv,j(T), have been determined. We show that rotational excitation of the reactants is as effective as vibrational excitation when it comes to increasing the reactivity, and that both types of excitation could contribute to explain the unexpectedly high abundance of CH(+) in the interstellar media. Such an increase in reactivity takes place by suppressing the reaction threshold when the internal energy is sufficient to overcome the endothermicity. Whenever this is the case, the excitation functions at collision energies Ecoll ≤ 0.1 eV display a ∝E(-1/2)coll dependence. However, the absolute values of the state selected kv=1(T) are one order of magnitude below the Langevin model predictions. The disagreement between the approximately derived experimental rate coefficients for v = 1 and those calculated by this and previous theoretical treatments is due to the neglect of the effect of the rotational excitation in the derivation of the former. In spite of the deep well present in the potential energy surface, the reaction does not show a statistical behaviour.

  15. Cord Blood Acute Phase Reactants Predict Early Onset Neonatal Sepsis in Preterm Infants.

    PubMed

    Mithal, Leena B; Palac, Hannah L; Yogev, Ram; Ernst, Linda M; Mestan, Karen K

    2017-01-01

    Early onset sepsis (EOS) is a major cause of morbidity and mortality in preterm infants, yet diagnosis remains inadequate resulting in missed cases or prolonged empiric antibiotics with adverse consequences. Evaluation of acute phase reactant (APR) biomarkers in umbilical cord blood at birth may improve EOS detection in preterm infants with intrauterine infection. In this nested case-control study, infants (29.7 weeks gestation, IQR: 27.7-32.2) were identified from a longitudinal cohort with archived cord blood and placental histopathology. Patients were categorized using culture, laboratory, clinical, and antibiotic treatment data into sepsis groups: confirmed sepsis (cEOS, n = 12); presumed sepsis (PS, n = 30); and no sepsis (controls, n = 30). Nine APRs were measured in duplicate from cord blood using commercially available multiplex immunoassays (Bio-Plex Pro™). In addition, placental histopathologic data were linked to biomarker results. cEOS organisms were Escherichia coli, Streptococcus agalactiae, Proteus mirabilis, Haemophilus influenzae and Listeria monocytogenes. C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin (Hp), serum amyloid P and ferritin were significantly elevated in cEOS compared to controls (p<0.01). SAA, CRP, and Hp were elevated in cEOS but not in PS (p<0.01) and had AUCs of 99%, 96%, and 95% respectively in predicting cEOS. Regression analysis revealed robust associations of SAA, CRP, and Hp with EOS after adjustment for covariates. Procalcitonin, fibrinogen, α-2-macroglobulin and tissue plasminogen activator were not significantly different across groups. Placental acute inflammation was associated with APR elevation and was present in all cEOS, 9 PS, and 17 control infants. This study shows that certain APRs are elevated in cord blood of premature infants with EOS of intrauterine origin. SAA, CRP, and Hp at birth have potential diagnostic utility for risk stratification and identification of infants with EOS.

  16. Cord Blood Acute Phase Reactants Predict Early Onset Neonatal Sepsis in Preterm Infants

    PubMed Central

    Palac, Hannah L.; Yogev, Ram; Ernst, Linda M.; Mestan, Karen K.

    2017-01-01

    Background Early onset sepsis (EOS) is a major cause of morbidity and mortality in preterm infants, yet diagnosis remains inadequate resulting in missed cases or prolonged empiric antibiotics with adverse consequences. Evaluation of acute phase reactant (APR) biomarkers in umbilical cord blood at birth may improve EOS detection in preterm infants with intrauterine infection. Methods In this nested case-control study, infants (29.7 weeks gestation, IQR: 27.7–32.2) were identified from a longitudinal cohort with archived cord blood and placental histopathology. Patients were categorized using culture, laboratory, clinical, and antibiotic treatment data into sepsis groups: confirmed sepsis (cEOS, n = 12); presumed sepsis (PS, n = 30); and no sepsis (controls, n = 30). Nine APRs were measured in duplicate from cord blood using commercially available multiplex immunoassays (Bio-Plex Pro™). In addition, placental histopathologic data were linked to biomarker results. Results cEOS organisms were Escherichia coli, Streptococcus agalactiae, Proteus mirabilis, Haemophilus influenzae and Listeria monocytogenes. C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin (Hp), serum amyloid P and ferritin were significantly elevated in cEOS compared to controls (p<0.01). SAA, CRP, and Hp were elevated in cEOS but not in PS (p<0.01) and had AUCs of 99%, 96%, and 95% respectively in predicting cEOS. Regression analysis revealed robust associations of SAA, CRP, and Hp with EOS after adjustment for covariates. Procalcitonin, fibrinogen, α-2-macroglobulin and tissue plasminogen activator were not significantly different across groups. Placental acute inflammation was associated with APR elevation and was present in all cEOS, 9 PS, and 17 control infants. Conclusion This study shows that certain APRs are elevated in cord blood of premature infants with EOS of intrauterine origin. SAA, CRP, and Hp at birth have potential diagnostic utility for risk stratification and

  17. [Application of the QIAamp DNA Investigator Kit and Prepfiler Forensic DNA Extraction Kit in genomic DNA extraction from skeletal remains].

    PubMed

    Ludwikowska-Pawłowska, Małgorzata; Jacewicz, Renata; Jedrzejczyk, Maciej; Prośniak, Adam; Berent, Jarosław

    2009-01-01

    The report presents an application of the QIAamp DNA Investigator Kit and PrepFiler Forensic DNA Extraction Kit in genomic DNA extraction from post-mortem highly degraded skeletal remains. The analysis included 25 bone samples collected on autopsy. DNA extraction was performed in accordance with the QIAamp DNA Investigator Kit and PrepFiler Forensic DNA Extraction Kit manufacturer's isolation protocols. Amplification was performed on a Biometra termocycler using the AmpFISTR Identifiler PCR Amplification Kit according to the manufacturer's protocol. Typing of PCR products was carried out on an ABI Prism 377 DNA sequencer. The recommended parameters for GeneScan analysis and Genotyper software were followed. The authors demonstrated that the QIAamp DNA Investigator Kit was more effective, convenient and statistically significantly better method which may be employed in DNA extraction from bone specimens.

  18. Wafer edge protection kit for MEMS and TSV Si-etching

    NASA Astrophysics Data System (ADS)

    Wieland, Robert; Nguyen, K.; Seidelmann, U.; Scholz, M.; Schrag, G.

    2015-05-01

    A new process kit for a SPTS Pegasus DRIE Si-Etch tool has been developed and tested for several different process regimes, e.g. bulk-Si cavity etching and TSV (through-Silicon-Via) etching with high aspect ratios <10:1, using the socalled Bosch process. Additionally, Si-etch back (recess etching) with a single step process has been tested as well. The especially developed "edge protection kit", consisting of Al2O3 material and optionally of PEEK material, covers the edge of a wafer, preventing it from being etched or even being etched away. However, placing such a part on top of the cathode, results in changes of the electric field distribution and the gas flow behavior compared to the standard process kit supplied by SPTS. The consequences may be altered Si-etch rates combined with changes of the tilt and side wall taper of the etched structures, mainly near the outside regions of the wafer. To this end, extensive investigations on the mask and bulk-Si etch rates, the tilt and taper angle of various MEMS test structures and their respective uniformity over the wafer surface have been performed. Additionally, simulations applying Comsol Multiphysics have been carried out to visualize the potential impact of the new process kit on the electrical field distribution. A simplex-optimization was carried out, varying the platen power and source power, in order to improve the tilt and to maintain the proper taper angle. One major advantage of the new process kit design compared to the original one is the reduction of movable parts to a minimum.

  19. c-Kit expression in desmoid fibromatosis. Comparative immunohistochemical evaluation of two commercial antibodies.

    PubMed

    Lucas, David R; al-Abbadi, Mousa; Tabaczka, Pamela; Hamre, Merlin R; Weaver, Donald W; Mott, Michael J

    2003-03-01

    To determine the frequency of c-Kit staining in desmoids and optimize an assay for clinical use, we stained 19 desmoids from various sites at various dilutions with 2 commonly used rabbit polyclonal, anti-c-Kit antibodies (A4502, DAKO, Carpinteria, CA; C-19, Santa Cruz Biotechnology, Santa Cruz, CA), with and without heat-induced epitope retrieval (HIER) in citrate buffer. Approdpriate external and internal control samples were evaluated for each test condition. At dilutions of 1:50 both antibodies stained substantial numbers of desmoids: with/without HIER, A4502, 89%/63%; C-19, 37%/74%. The staining was cytoplasmic without cell membrane accentuation. However, background stromal staining and nonspecific staining of endothelium and smooth and striated muscle were problematic with both antibodies at 1:50. At higher dilutions, C-19 stained no desmoid; however, diminished staining of external and internal control samples made it unreliable. A4502 similarly stained many fewer desmoids at higher dilutions. However, it retained strong staining of both external and internal control samples and showed much less nonspecific staining. Best results were achieved at 1:250 without HIER; only weak focal staining was present in 1 desmoid. With a simple immunohistochemical method optimized for clinical use, desmoid can be regarded as a c-Kit-negative tumor.

  20. EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.

    PubMed

    Moore, Simon J; Lai, Hung-En; Kelwick, Richard J R; Chee, Soo Mei; Bell, David J; Polizzi, Karen Marie; Freemont, Paul S

    2016-10-21

    Golden Gate cloning is a prominent DNA assembly tool in synthetic biology for the assembly of plasmid constructs often used in combinatorial pathway optimization, with a number of assembly kits developed specifically for yeast and plant-based expression. However, its use for synthetic biology in commonly used bacterial systems such as Escherichia coli has surprisingly been overlooked. Here, we introduce EcoFlex a simplified modular package of DNA parts for a variety of applications in E. coli, cell-free protein synthesis, protein purification and hierarchical assembly of transcription units based on the MoClo assembly standard. The kit features a library of constitutive promoters, T7 expression, RBS strength variants, synthetic terminators, protein purification tags and fluorescence proteins. We validate EcoFlex by assembling a 68-part containing (20 genes) plasmid (31 kb), characterize in vivo and in vitro library parts, and perform combinatorial pathway assembly, using pooled libraries of either fluorescent proteins or the biosynthetic genes for the antimicrobial pigment violacein as a proof-of-concept. To minimize pathway screening, we also introduce a secondary module design site to simplify MoClo pathway optimization. In summary, EcoFlex provides a standardized and multifunctional kit for a variety of applications in E. coli synthetic biology.

  1. Developmental validation of a forensic rapid DNA-STR kit: Expressmarker 16.

    PubMed

    Zhou, Huaigu; Wu, Dan; Chen, Ronghua; Xu, Yan; Xia, Zifang; Guo, Yulin; Zhang, Fan; Zheng, Weiguo

    2014-07-01

    DNA-STR analysis is widely used in the forensic science field and has important requirements on the analysis time to obtain faster inspections. The developed forensic STR kit, referred to as Expressmarker 16 (EX16), could shorten the amplification time to a minimum of 35min. It enables 16 STR loci to be co-detected, including 13 CODIS loci, D2S1338, D6S1043 and Amelogenin loci. The kit is validated by a series of tests formed by DNA mixtures, stutter ratios, optimized PCR protocols based on annealing temperature research, species specificities, inhibitors, sensitivity, and parallel tests according to FBI QAS (2009/2011) (QAS, 2009; SWGDAM, 2010). The results demonstrated that EX16 was a useful tool for rapid criminal investigation.

  2. Structure-based de novo design and identification of D816V mutant-selective c-KIT inhibitors.

    PubMed

    Park, Hwangseo; Lee, Soyoung; Lee, Suhyun; Hong, Sungwoo

    2014-07-14

    To identify potent and selective inhibitors of D816V, the most common gain-of-function c-KIT mutant, we carried out structure-based de novo design using 7-azaindole as the core and the scoring function improved by implementing an accurate solvation free energy term. This approach led to the identification of new c-KIT inhibitors specific for the D816V mutant. The 3-(3,4-dimethoxyphenyl)-7-azaindole scaffold was optimized and represents a lead structure for the design of the potent and specific inhibitors of the D816V mutant. The results of molecular dynamics simulations indicate that hydrogen bonding interactions between the 7-azadindole moiety and the backbone groups of Cys673 are the most significant determinant for the potency and selectivity of c-KIT inhibitors.

  3. Experimental and numerical analysis of parallel reactant flow and transverse mixing with mineral precipitation in homogeneous and heterogeneous porous media

    DOE PAGES

    Fox, Don T.; Guo, Luanjing; Fujita, Yoshiko; ...

    2015-12-17

    Formation of mineral precipitates in the mixing interface between two reactant solutions flowing in parallel in porous media is governed by reactant mixing by diffusion and dispersion and is coupled to changes in porosity/permeability due to precipitation. The spatial and temporal distribution of mixing-dependent precipitation of barium sulfate in porous media was investigated with side-by-side injection of barium chloride and sodium sulfate solutions in thin rectangular flow cells packed with quartz sand. The results for homogeneous sand beds were compared to beds with higher or lower permeability inclusions positioned in the path of the mixing zone. In the homogeneous andmore » high permeability inclusion experiments, BaSO4 precipitate (barite) formed in a narrow deposit along the length and in the center of the solution–solution mixing zone even though dispersion was enhanced within, and downstream of, the high permeability inclusion. In the low permeability inclusion experiment, the deflected BaSO4 precipitation zone broadened around one side and downstream of the inclusion and was observed to migrate laterally toward the sulfate solution. A continuum-scale fully coupled reactive transport model that simultaneously solves the nonlinear governing equations for fluid flow, transport of reactants and geochemical reactions was used to simulate the experiments and provide insight into mechanisms underlying the experimental observations. Lastly, migration of the precipitation zone in the low permeability inclusion experiment could be explained by the coupling effects among fluid flow, reactant transport and localized mineral precipitation reaction.« less

  4. Detailed high-resolution three-dimensional simulations of OMEGA separated reactants inertial confinement fusion experiments

    NASA Astrophysics Data System (ADS)

    Haines, Brian M.; Grim, Gary P.; Fincke, James R.; Shah, Rahul C.; Forrest, Chad J.; Silverstein, Kevin; Marshall, Frederic J.; Boswell, Melissa; Fowler, Malcolm M.; Gore, Robert A.; Hayes-Sterbenz, Anna C.; Jungman, Gerard; Klein, Andreas; Rundberg, Robert S.; Steinkamp, Michael J.; Wilhelmy, Jerry B.

    2016-07-01

    We present results from the comparison of high-resolution three-dimensional (3D) simulations with data from the implosions of inertial confinement fusion capsules with separated reactants performed on the OMEGA laser facility. Each capsule, referred to as a "CD Mixcap," is filled with tritium and has a polystyrene (CH) shell with a deuterated polystyrene (CD) layer whose burial depth is varied. In these implosions, fusion reactions between deuterium and tritium ions can occur only in the presence of atomic mix between the gas fill and shell material. The simulations feature accurate models for all known experimental asymmetries and do not employ any adjustable parameters to improve agreement with experimental data. Simulations are performed with the RAGE radiation-hydrodynamics code using an Implicit Large Eddy Simulation (ILES) strategy for the hydrodynamics. We obtain good agreement with the experimental data, including the DT/TT neutron yield ratios used to diagnose mix, for all burial depths of the deuterated shell layer. Additionally, simulations demonstrate good agreement with converged simulations employing explicit models for plasma diffusion and viscosity, suggesting that the implicit sub-grid model used in ILES is sufficient to model these processes in these experiments. In our simulations, mixing is driven by short-wavelength asymmetries and longer-wavelength features are responsible for developing flows that transport mixed material towards the center of the hot spot. Mix material transported by this process is responsible for most of the mix (DT) yield even for the capsule with a CD layer adjacent to the tritium fuel. Consistent with our previous results, mix does not play a significant role in TT neutron yield degradation; instead, this is dominated by the displacement of fuel from the center of the implosion due to the development of turbulent instabilities seeded by long-wavelength asymmetries. Through these processes, the long

  5. Soft X-Ray Photochemistry of Condensed Mixed Reactants: Thin Film Growth and Ion Desorption

    NASA Astrophysics Data System (ADS)

    Moore, Jerry Franklin, Jr.

    reactants will be discussed.

  6. Detailed high-resolution three-dimensional simulations of OMEGA separated reactants inertial confinement fusion experiments

    SciTech Connect

    Haines, Brian M.; Grim, Gary P.; Fincke, James R.; Shah, Rahul C.; Forrest, Chad J.; Silverstein, Kevin; Marshall, Frederic J.; Boswell, Melissa; Fowler, Malcolm M.; Gore, Robert A.; Hayes-Sterbenz, Anna C.; Jungman, Gerard; Klein, Andreas; Rundberg, Robert S.; Steinkamp, Michael J.; Wilhelmy, Jerry B.

    2016-07-01

    We present results from the comparison of high-resolution three-dimensional (3D) simulations with data from the implosions of inertial confinement fusion capsules with separated reactants performed on the OMEGA laser facility. Each capsule, referred to as a “CD Mixcap,” is filled with tritium and has a polystyrene (CH) shell with a deuterated polystyrene (CD) layer whose burial depth is varied. In these implosions, fusion reactions between deuterium and tritium ions can occur only in the presence of atomic mix between the gas fill and shell material. The simulations feature accurate models for all known experimental asymmetries and do not employ any adjustable parameters to improve agreement with experimental data. Simulations are performed with the RAGE radiation-hydrodynamics code using an Implicit Large Eddy Simulation (ILES) strategy for the hydrodynamics. We obtain good agreement with the experimental data, including the DT/TT neutron yield ratios used to diagnose mix, for all burial depths of the deuterated shell layer. Additionally, simulations demonstrate good agreement with converged simulations employing explicit models for plasma diffusion and viscosity, suggesting that the implicit sub-grid model used in ILES is sufficient to model these processes in these experiments. In our simulations, mixing is driven by short-wavelength asymmetries and longer-wavelength features are responsible for developing flows that transport mixed material towards the center of the hot spot. Mix material transported by this process is responsible for most of the mix (DT) yield even for the capsule with a CD layer adjacent to the tritium fuel. Consistent with our previous results, mix does not play a significant role in TT neutron yield degradation; instead, this is dominated by the displacement of fuel from the center of the implosion due to the development of turbulent instabilities seeded by long-wavelength asymmetries. Through these processes, the long

  7. Detailed high-resolution three-dimensional simulations of OMEGA separated reactants inertial confinement fusion experiments

    DOE PAGES

    Haines, Brian Michael; Grim, Gary P.; Fincke, James R.; ...

    2016-07-29

    Here, we present results from the comparison of high-resolution three-dimensional (3D) simulations with data from the implosions of inertial confinement fusion capsules with separated reactants performed on the OMEGA laser facility. Each capsule, referred to as a “CD Mixcap,” is filled with tritium and has a polystyrene (CH) shell with a deuterated polystyrene (CD) layer whose burial depth is varied. In these implosions, fusion reactions between deuterium and tritium ions can occur only in the presence of atomic mix between the gas fill and shell material. The simulations feature accurate models for all known experimental asymmetries and do not employmore » any adjustable parameters to improve agreement with experimental data. Simulations are performed with the RAGE radiation-hydrodynamics code using an Implicit Large Eddy Simulation (ILES) strategy for the hydrodynamics. We obtain good agreement with the experimental data, including the DT/TT neutron yield ratios used to diagnose mix, for all burial depths of the deuterated shell layer. Additionally, simulations demonstrate good agreement with converged simulations employing explicit models for plasma diffusion and viscosity, suggesting that the implicit sub-grid model used in ILES is sufficient to model these processes in these experiments. In our simulations, mixing is driven by short-wavelength asymmetries and longer-wavelength features are responsible for developing flows that transport mixed material towards the center of the hot spot. Mix material transported by this process is responsible for most of the mix (DT) yield even for the capsule with a CD layer adjacent to the tritium fuel. Consistent with our previous results, mix does not play a significant role in TT neutron yield degradation; instead, this is dominated by the displacement of fuel from the center of the implosion due to the development of turbulent instabilities seeded by long-wavelength asymmetries. Through these processes, the long

  8. Detailed high-resolution three-dimensional simulations of OMEGA separated reactants inertial confinement fusion experiments

    SciTech Connect

    Haines, Brian Michael; Grim, Gary P.; Fincke, James R.; Shah, Rahul C.; Forrest, Chad J.; Silverstein, Kevin; Marshall, Frederic J.; Boswell, Melissa; Fowler, Malcolm M.; Gore, Robert A.; Hayes-Sterbenz, Anna Catherine; Jungman, Gerard; Klein, Andreas; Rundberg, Robert S.; Steinkamp, Michael James; Wilhelmy, Jerry B.

    2016-07-29

    Here, we present results from the comparison of high-resolution three-dimensional (3D) simulations with data from the implosions of inertial confinement fusion capsules with separated reactants performed on the OMEGA laser facility. Each capsule, referred to as a “CD Mixcap,” is filled with tritium and has a polystyrene (CH) shell with a deuterated polystyrene (CD) layer whose burial depth is varied. In these implosions, fusion reactions between deuterium and tritium ions can occur only in the presence of atomic mix between the gas fill and shell material. The simulations feature accurate models for all known experimental asymmetries and do not employ any adjustable parameters to improve agreement with experimental data. Simulations are performed with the RAGE radiation-hydrodynamics code using an Implicit Large Eddy Simulation (ILES) strategy for the hydrodynamics. We obtain good agreement with the experimental data, including the DT/TT neutron yield ratios used to diagnose mix, for all burial depths of the deuterated shell layer. Additionally, simulations demonstrate good agreement with converged simulations employing explicit models for plasma diffusion and viscosity, suggesting that the implicit sub-grid model used in ILES is sufficient to model these processes in these experiments. In our simulations, mixing is driven by short-wavelength asymmetries and longer-wavelength features are responsible for developing flows that transport mixed material towards the center of the hot spot. Mix material transported by this process is responsible for most of the mix (DT) yield even for the capsule with a CD layer adjacent to the tritium fuel. Consistent with our previous results, mix does not play a significant role in TT neutron yield degradation; instead, this is dominated by the displacement of fuel from the center of the implosion due to the development of turbulent instabilities seeded by long-wavelength asymmetries. Through these processes, the long

  9. Detailed high-resolution three-dimensional simulations of OMEGA separated reactants inertial confinement fusion experiments

    SciTech Connect

    Haines, Brian Michael; Grim, Gary P.; Fincke, James R.; Shah, Rahul C.; Forrest, Chad J.; Silverstein, Kevin; Marshall, Frederic J.; Boswell, Melissa; Fowler, Malcolm M.; Gore, Robert A.; Hayes-Sterbenz, Anna Catherine; Jungman, Gerard; Klein, Andreas; Rundberg, Robert S.; Steinkamp, Michael James; Wilhelmy, Jerry B.

    2016-07-29

    Here, we present results from the comparison of high-resolution three-dimensional (3D) simulations with data from the implosions of inertial confinement fusion capsules with separated reactants performed on the OMEGA laser facility. Each capsule, referred to as a “CD Mixcap,” is filled with tritium and has a polystyrene (CH) shell with a deuterated polystyrene (CD) layer whose burial depth is varied. In these implosions, fusion reactions between deuterium and tritium ions can occur only in the presence of atomic mix between the gas fill and shell material. The simulations feature accurate models for all known experimental asymmetries and do not employ any adjustable parameters to improve agreement with experimental data. Simulations are performed with the RAGE radiation-hydrodynamics code using an Implicit Large Eddy Simulation (ILES) strategy for the hydrodynamics. We obtain good agreement with the experimental data, including the DT/TT neutron yield ratios used to diagnose mix, for all burial depths of the deuterated shell layer. Additionally, simulations demonstrate good agreement with converged simulations employing explicit models for plasma diffusion and viscosity, suggesting that the implicit sub-grid model used in ILES is sufficient to model these processes in these experiments. In our simulations, mixing is driven by short-wavelength asymmetries and longer-wavelength features are responsible for developing flows that transport mixed material towards the center of the hot spot. Mix material transported by this process is responsible for most of the mix (DT) yield even for the capsule with a CD layer adjacent to the tritium fuel. Consistent with our previous results, mix does not play a significant role in TT neutron yield degradation; instead, this is dominated by the displacement of fuel from the center of the implosion due to the development of turbulent instabilities seeded by long-wavelength asymmetries. Through these processes, the long

  10. Acute-phase reactants in acute myocardial infarction: impact on 5-year prognosis.

    PubMed

    Mariotti, R; Musumeci, G; De Carlo, M; Biadi, O; Caravelli, P; Limbruno, U; Mariani, M

    2001-04-01

    Acute-phase reactants have recently been shown to have a short-term and possibly long-term prognostic value in acute coronary syndromes. The aim of the present study was to retrospectively verify whether serum levels of inflammation markers can predict the occurrence of early and late cardiac events after myocardial infarction. We reevaluated 58 consecutive patients (43 men and 15 women, mean age 66 +/- 12 years) admitted to our Center during 1993 with a first myocardial infarction. Patients with non-cardiac causes of inflammation were excluded, as well as patients with a left ventricular ejection fraction <40%. From the first blood sample obtained at admission, we evaluated C-reactive protein (CRP) and alpha1-acid glycoprotein (alpha1-AGP) serum levels, the erythrocyte sedimentation rate (ESR), fibrinogen levels, and the white blood cell (WBC) count. We also evaluated the highest level of serum cardiac markers. Follow-up data were collected for 55 patients in June 1999. Five in-hospital and 13 delayed cardiac deaths occurred. The mean follow-up of current survivors was 5.9 +/- 0.4 years. Patients in whom cardiac death occurred had significantly higher CRP (7.4 +/- 4.1 vs 3.0 +/- 2.4 mg/dl, p < 0.001) and alpha1-AGP levels (160 +/- 38 vs 113 +/- 24 mg/dl, p < 0.001), ESR (63 +/- 30 vs 37 +/- 25 mm/hour, p < 0.001), and WBC count (13,727 +/- 3,853 vs 10,936 +/- 3,358/mm3, p = 0.004). At multivariate analysis, higher alpha1-AGP (p < 0.001) and CRP serum levels (p = 0.02) were independent predictors of cardiac death. Patients in whom cardiac events occurred during follow-up showed higher CRP (5.7 +/- 3.7 vs 1.6 +/- 1.5 mg/dl, p < 0.001) and alpha1-AGP levels (140 +/- 36 vs 101 +/- 23 mg/dl, p < 0.001) and ESR (50 +/- 30 vs 34 +/- 26 mm/hour, p = 0.06). Higher alpha1-AGP (p < 0.001) and CRP serum levels (p = 0.03) were independent predictors of the occurrence of cardiac events. The present study shows that CRP and alpha1-AGP have an independent prognostic value in

  11. Detailed high-resolution three-dimensional simulations of OMEGA separated reactants inertial confinement fusion experiments

    SciTech Connect

    Haines, Brian M. Fincke, James R.; Shah, Rahul C.; Boswell, Melissa; Fowler, Malcolm M.; Gore, Robert A.; Hayes-Sterbenz, Anna C.; Jungman, Gerard; Klein, Andreas; Rundberg, Robert S.; Steinkamp, Michael J.; Wilhelmy, Jerry B.; Grim, Gary P.; Forrest, Chad J.; Silverstein, Kevin; Marshall, Frederic J.

    2016-07-15

    We present results from the comparison of high-resolution three-dimensional (3D) simulations with data from the implosions of inertial confinement fusion capsules with separated reactants performed on the OMEGA laser facility. Each capsule, referred to as a “CD Mixcap,” is filled with tritium and has a polystyrene (CH) shell with a deuterated polystyrene (CD) layer whose burial depth is varied. In these implosions, fusion reactions between deuterium and tritium ions can occur only in the presence of atomic mix between the gas fill and shell material. The simulations feature accurate models for all known experimental asymmetries and do not employ any adjustable parameters to improve agreement with experimental data. Simulations are performed with the RAGE radiation-hydrodynamics code using an Implicit Large Eddy Simulation (ILES) strategy for the hydrodynamics. We obtain good agreement with the experimental data, including the DT/TT neutron yield ratios used to diagnose mix, for all burial depths of the deuterated shell layer. Additionally, simulations demonstrate good agreement with converged simulations employing explicit models for plasma diffusion and viscosity, suggesting that the implicit sub-grid model used in ILES is sufficient to model these processes in these experiments. In our simulations, mixing is driven by short-wavelength asymmetries and longer-wavelength features are responsible for developing flows that transport mixed material towards the center of the hot spot. Mix material transported by this process is responsible for most of the mix (DT) yield even for the capsule with a CD layer adjacent to the tritium fuel. Consistent with our previous results, mix does not play a significant role in TT neutron yield degradation; instead, this is dominated by the displacement of fuel from the center of the implosion due to the development of turbulent instabilities seeded by long-wavelength asymmetries. Through these processes, the long

  12. 21 CFR 872.3570 - OTC denture repair kit.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false OTC denture repair kit. 872.3570 Section 872.3570 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES DENTAL DEVICES Prosthetic Devices § 872.3570 OTC denture repair kit. (a)...

  13. 21 CFR 872.3570 - OTC denture repair kit.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false OTC denture repair kit. 872.3570 Section 872.3570 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES DENTAL DEVICES Prosthetic Devices § 872.3570 OTC denture repair kit. (a)...

  14. 21 CFR 872.3570 - OTC denture repair kit.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false OTC denture repair kit. 872.3570 Section 872.3570 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES DENTAL DEVICES Prosthetic Devices § 872.3570 OTC denture repair kit. (a)...

  15. 21 CFR 872.3570 - OTC denture repair kit.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false OTC denture repair kit. 872.3570 Section 872.3570 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES DENTAL DEVICES Prosthetic Devices § 872.3570 OTC denture repair kit. (a)...

  16. EPIE Educational Product Report: Early Learning Kits -- 25 Evaluations.

    ERIC Educational Resources Information Center

    EPIE Educational Product Report, 1975

    1975-01-01

    This report presents evaluations of 25 early learning materials kits, designed for use with young children in nursery school, kindergarten, and day care centers. Each evaluation includes: a physical description of the kit (with cost information), the target audience, content and objectives, methods of use, conditions of use, rationale, research…

  17. Indoor Air Quality Tools for Schools Action Kit. Second Edition.

    ERIC Educational Resources Information Center

    Environmental Protection Agency, Washington, DC.

    This kit contains materials to assist a school indoor air quality (IAQ) coordinator in conducting a school IAQ program. The kit contains the following: IAQ coordinator's guide; IAQ coordinator forms; IAQ backgrounder; teacher's classroom checklist; administrative staff checklist; health officer/school nurse checklist; ventilation checklist and…

  18. Energy Education Incentives: Evaluating the Impact of Consumer Energy Kits

    ERIC Educational Resources Information Center

    Kirby, Sarah D.; Guin, Autumn; Langham, Laura

    2015-01-01

    Measuring the energy and environmental impact of residential energy education efforts is difficult. The E-Conservation residential energy management program uses consumer energy kits to document the impact of energy-efficient improvements. The consumer energy kit provides an incentive for individuals attending energy education workshop, helps…

  19. TQAP for Verification of Qualitative Lead Test Kits

    EPA Science Inventory

    There are lead-based paint test kits available to help home owners and contractors identify lead-based paint hazards before any Renovation, Repair, and Painting (RRP) activities take place so that proper health and safety meaures can be enacted. However, many of these test kits ...

  20. 14 CFR 1214.604 - Personal Preference Kit (PPK).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Personal Preference Kit (PPK). 1214.604... Aboard Space Shuttle Flights § 1214.604 Personal Preference Kit (PPK). (a) Purpose. The PPK enables... only by the Associate Administrator for Space Flight. (c) Approval of Contents. At least 60 days...

  1. 14 CFR 1214.603 - Official Flight Kit (OFK).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Official Flight Kit (OFK). 1214.603 Section... Aboard Space Shuttle Flights § 1214.603 Official Flight Kit (OFK). (a) Purpose. The OFK on a particular... addition to § 1214.602(c) of this part, U.S. national flags will not be flown as mementos except by...

  2. 14 CFR 1214.604 - Personal Preference Kit (PPK).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Personal Preference Kit (PPK). 1214.604... Aboard Space Shuttle Flights § 1214.604 Personal Preference Kit (PPK). (a) Purpose. The PPK enables... only by the Associate Administrator for Space Flight. (c) Approval of Contents. At least 60 days...

  3. 14 CFR § 1214.604 - Personal Preference Kit (PPK).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 5 2014-01-01 2014-01-01 false Personal Preference Kit (PPK). § 1214.604... Mementos Aboard Space Shuttle Flights § 1214.604 Personal Preference Kit (PPK). (a) Purpose. The PPK... only by the Associate Administrator for Space Flight. (c) Approval of Contents. At least 60 days...

  4. 14 CFR 1214.603 - Official Flight Kit (OFK).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Official Flight Kit (OFK). 1214.603 Section... Aboard Space Shuttle Flights § 1214.603 Official Flight Kit (OFK). (a) Purpose. The OFK on a particular... addition to § 1214.602(c) of this part, U.S. national flags will not be flown as mementos except by...

  5. 10 CFR 429.33 - Ceiling fan light kits.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 3 2013-01-01 2013-01-01 false Ceiling fan light kits. 429.33 Section 429.33 Energy... COMMERCIAL AND INDUSTRIAL EQUIPMENT Certification § 429.33 Ceiling fan light kits. (a) Sampling plan for selection of units for testing. (1) The requirements of § 429.11 are applicable to ceiling fan light...

  6. 10 CFR 429.33 - Ceiling fan light kits.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 3 2012-01-01 2012-01-01 false Ceiling fan light kits. 429.33 Section 429.33 Energy... COMMERCIAL AND INDUSTRIAL EQUIPMENT Certification § 429.33 Ceiling fan light kits. (a) Sampling plan for selection of units for testing. (1) The requirements of § 429.11 are applicable to ceiling fan light...

  7. 10 CFR 429.33 - Ceiling fan light kits.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 3 2014-01-01 2014-01-01 false Ceiling fan light kits. 429.33 Section 429.33 Energy... COMMERCIAL AND INDUSTRIAL EQUIPMENT Certification § 429.33 Ceiling fan light kits. (a) Sampling plan for selection of units for testing. (1) The requirements of § 429.11 are applicable to ceiling fan light...

  8. Reengaging Disconnected Youth. Action Kit for Municipal Leaders. Issue #7

    ERIC Educational Resources Information Center

    Johnson, Alicia

    2005-01-01

    This action kit highlights steps city officials can take to help young people who are not connected to school, work, or caring adults. The kit recommends steps that cities can take to promote educational achievement, develop stronger workforce connections, support youth in transition from the foster care and juvenile justice systems, and build a…

  9. Computer-Based Assessment of Cognition: The ETS Factor Kit.

    ERIC Educational Resources Information Center

    Ekstrom, Ruth B.; Bejar, Isaac I.

    The history of the Educational Testing Service (ETS) Factor Kits is summarized. The original ETS Factor Kit was developed in 1954 and contained 51 items, three each for each of 15 factors and six for a 16th factor. The next edition was developed in 1963 and included adaptations (clones) of the defining tests instead of the exact copies. These…

  10. Organization Charts in ARL Libraries. SPEC Kit #170.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This kit is based on a review of the organization charts of 71 member libraries of the Association of Research Libraries (ARL) gathered in January 1991, compared with an earlier SPEC Kit published in 1986, and contains charts of 29 of the libraries. A summary of the chart analyses presents information about the titles of library directors,…

  11. 33 CFR 144.01-30 - First-aid kit.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false First-aid kit. 144.01-30 Section 144.01-30 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES LIFESAVING APPLIANCES Manned Platforms § 144.01-30 First-aid kit....

  12. School-to-Work Jump-Start Equity Kit.

    ERIC Educational Resources Information Center

    Education Development Center, Inc., Newton, MA. Women's Educational Equity Act Dissemination Center.

    This starter kit is a resource for state and local school-to-work (STW) directors, educators, parents, students, business and community, and economic development organizations serving all students through STW. The kit begins with four articles: "STW and Gender Equity: Opportunity for or Barrier to Economic Parity?" (Katherine Hanson, Joyce…

  13. Take Two People--A Genetics Teaching Kit.

    ERIC Educational Resources Information Center

    Dixon, Rosy

    1982-01-01

    "Take Two People" is a kit of model genes which can be made up in packs to represent people or gametes. The kit is simple to make and highly adaptable. It has been useful and effective in teaching genetics to students of low ability and little motivation. (Author/JN)

  14. 14 CFR 1214.603 - Official Flight Kit (OFK).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Official Flight Kit (OFK). 1214.603 Section 1214.603 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT Mementos Aboard Space Shuttle Flights § 1214.603 Official Flight Kit (OFK). (a) Purpose. The OFK on a...

  15. 14 CFR 1214.603 - Official Flight Kit (OFK).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Official Flight Kit (OFK). 1214.603 Section 1214.603 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT Mementos Aboard Space Shuttle Flights § 1214.603 Official Flight Kit (OFK). (a) Purpose. The OFK on a...

  16. On-Line Bibliographic Search Services. SPEC Kit 62.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This Systems and Procedures Exchange Center (SPEC) kit examines the current state of online services based upon the experiences of a select number of Association of Research Libraries (ARL) member libraries in the United States and Canada. Many of the kit documents were obtained during a survey of eight private institutions conducted for Duke…

  17. Neutral Buoyancy Simulator - Fluid line repair kit development

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Marshall's Neutral Buoyancy Simulator (NBS) is used to simulate the gravitational fields and buoyancy effects outer space has on astronauts and their ability to perform tasks in this environment. In this example, a diver performs a temporary fluid line repair task using a repair kit developed by Marshall engineers. The analysis will determine the value of this repair kit and its feasibility.

  18. EPIE Educational Product Report: Early Learning Kits -- 25 Evaluations.

    ERIC Educational Resources Information Center

    EPIE Educational Product Report, 1975

    1975-01-01

    This report presents evaluations of 25 early learning materials kits, designed for use with young children in nursery school, kindergarten, and day care centers. Each evaluation includes: a physical description of the kit (with cost information), the target audience, content and objectives, methods of use, conditions of use, rationale, research…

  19. A Survival Kit for Teaching English to Refugees. TECHNIQUES.

    ERIC Educational Resources Information Center

    Overbeck, Carla

    1984-01-01

    Adult basic education and English as a second language teachers, as well as volunteer tutors, can help new refugees acquire functional English through the use of a survival kit. The Literacy Volunteers of America's guide to teaching conversational English recommends these items for a survival kit: a written copy of student's name, address, and…

  20. TQAP for Verification of Qualitative Lead Test Kits

    EPA Science Inventory

    There are lead-based paint test kits available to help home owners and contractors identify lead-based paint hazards before any Renovation, Repair, and Painting (RRP) activities take place so that proper health and safety meaures can be enacted. However, many of these test kits ...