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Sample records for real time mass

  1. Single-protein nanomechanical mass spectrometry in real time

    PubMed Central

    Hanay, M.S.; Kelber, S.; Naik, A.K.; Chi, D.; Hentz, S.; Bullard, E.C.; Colinet, E.; Duraffourg, L.; Roukes, M.L.

    2012-01-01

    Nanoelectromechanical systems (NEMS) resonators can detect mass with exceptional sensitivity. Previously, mass spectra from several hundred adsorption events were assembled in NEMS-based mass spectrometry using statistical analysis. Here, we report the first realization of single-molecule NEMS-based mass spectrometry in real time. As each molecule in the sample adsorbs upon the NEMS resonator, its mass and the position-of-adsorption are determined by continuously tracking two driven vibrational modes of the device. We demonstrate the potential of multimode NEMS-based mass spectrometry by analyzing IgM antibody complexes in real-time. NEMS-MS is a unique and promising new form of mass spectrometry: it can resolve neutral species, provides resolving power that increases markedly for very large masses, and allows acquisition of spectra, molecule-by-molecule, in real-time. PMID:22922541

  2. Real-time PCR and PCR-tandem Mass Spectrometry for Biodetection

    DTIC Science & Technology

    2005-10-01

    Real - time PCR and PCR- tandem mass spectrometry for biodetection Alvin Fox, University of South Carolina, School of Medicine Report Documentation...TYPE N/A 3. DATES COVERED - 4. TITLE AND SUBTITLE Real - time PCR and PCRtandem mass spectrometry for biodetection 5a. CONTRACT NUMBER 5b...interspace region Bacillus subtilis W23 standard Blank Barn dust House dust Cycle Real - time PCR (16s rRNA) - environmental samples Real - time

  3. Mass spectrometer for real-time metabolism monitoring during anesthesia

    NASA Astrophysics Data System (ADS)

    Elizarov, A. Yu.; Levshankov, A. I.

    2012-06-01

    Mass-spectrometric monitoring of metabolism (CO2/O2) in the inspiration-expiration regime is used to estimate the anesthetic protection of the patient against surgical stimulation during combined anesthesia. A correlation between the anesthetic protection of the patient and the metabolic rate is demonstrated, and the periodic variation of the metabolic rate with time is found. The sevoflurane metabolism products and intravenous analgesic fentanyl are found in the blowing air of the patient during anesthesia.

  4. In vivo Real-Time Mass Spectrometry for Guided Surgery Application

    NASA Astrophysics Data System (ADS)

    Fatou, Benoit; Saudemont, Philippe; Leblanc, Eric; Vinatier, Denis; Mesdag, Violette; Wisztorski, Maxence; Focsa, Cristian; Salzet, Michel; Ziskind, Michael; Fournier, Isabelle

    2016-05-01

    Here we describe a new instrument (SpiderMass) designed for in vivo and real-time analysis. In this instrument ion production is performed remotely from the MS instrument and the generated ions are transported in real-time to the MS analyzer. Ion production is promoted by Resonant Infrared Laser Ablation (RIR-LA) based on the highly effective excitation of O-H bonds in water molecules naturally present in most biological samples. The retrieved molecular patterns are specific to the cell phenotypes and benign versus cancer regions of patient biopsies can be easily differentiated. We also demonstrate by analysis of human skin that SpiderMass can be used under in vivo conditions with minimal damage and pain. Furthermore SpiderMass can also be used for real-time drug metabolism and pharmacokinetic (DMPK) analysis or food safety topics. SpiderMass is thus the first MS based system designed for in vivo real-time analysis under minimally invasive conditions.

  5. In vivo Real-Time Mass Spectrometry for Guided Surgery Application

    PubMed Central

    Fatou, Benoit; Saudemont, Philippe; Leblanc, Eric; Vinatier, Denis; Mesdag, Violette; Wisztorski, Maxence; Focsa, Cristian; Salzet, Michel; Ziskind, Michael; Fournier, Isabelle

    2016-01-01

    Here we describe a new instrument (SpiderMass) designed for in vivo and real-time analysis. In this instrument ion production is performed remotely from the MS instrument and the generated ions are transported in real-time to the MS analyzer. Ion production is promoted by Resonant Infrared Laser Ablation (RIR-LA) based on the highly effective excitation of O-H bonds in water molecules naturally present in most biological samples. The retrieved molecular patterns are specific to the cell phenotypes and benign versus cancer regions of patient biopsies can be easily differentiated. We also demonstrate by analysis of human skin that SpiderMass can be used under in vivo conditions with minimal damage and pain. Furthermore SpiderMass can also be used for real-time drug metabolism and pharmacokinetic (DMPK) analysis or food safety topics. SpiderMass is thus the first MS based system designed for in vivo real-time analysis under minimally invasive conditions. PMID:27189490

  6. Real time monitoring of accelerated chemical reactions by ultrasonication-assisted spray ionization mass spectrometry.

    PubMed

    Lin, Shu-Hsuan; Lo, Ta-Ju; Kuo, Fang-Yin; Chen, Yu-Chie

    2014-01-01

    Ultrasonication has been used to accelerate chemical reactions. It would be ideal if ultrasonication-assisted chemical reactions could be monitored by suitable detection tools such as mass spectrometry in real time. It would be helpful to clarify reaction intermediates/products and to have a better understanding of reaction mechanism. In this work, we developed a system for ultrasonication-assisted spray ionization mass spectrometry (UASI-MS) with an ~1.7 MHz ultrasonic transducer to monitor chemical reactions in real time. We demonstrated that simply depositing a sample solution on the MHz-based ultrasonic transducer, which was placed in front of the orifice of a mass spectrometer, the analyte signals can be readily detected by the mass spectrometer. Singly and multiply charged ions from small and large molecules, respectively, can be observed in the UASI mass spectra. Furthermore, the ultrasonic transducer used in the UASI setup accelerates the chemical reactions while being monitored via UASI-MS. The feasibility of using this approach for real-time acceleration/monitoring of chemical reactions was demonstrated. The reactions of Girard T reagent and hydroxylamine with steroids were used as the model reactions. Upon the deposition of reactant solutions on the ultrasonic transducer, the intermediate/product ions are readily generated and instantaneously monitored using MS within 1 s. Additionally, we also showed the possibility of using this reactive UASI-MS approach to assist the confirmation of trace steroids from complex urine samples by monitoring the generation of the product ions.

  7. Portable Dew Point Mass Spectrometry System for Real-Time Gas and Moisture Analysis

    NASA Technical Reports Server (NTRS)

    Arkin, C.; Gillespie, Stacey; Ratzel, Christopher

    2010-01-01

    A portable instrument incorporates both mass spectrometry and dew point measurement to provide real-time, quantitative gas measurements of helium, nitrogen, oxygen, argon, and carbon dioxide, along with real-time, quantitative moisture analysis. The Portable Dew Point Mass Spectrometry (PDP-MS) system comprises a single quadrupole mass spectrometer and a high vacuum system consisting of a turbopump and a diaphragm-backing pump. A capacitive membrane dew point sensor was placed upstream of the MS, but still within the pressure-flow control pneumatic region. Pressure-flow control was achieved with an upstream precision metering valve, a capacitance diaphragm gauge, and a downstream mass flow controller. User configurable LabVIEW software was developed to provide real-time concentration data for the MS, dew point monitor, and sample delivery system pressure control, pressure and flow monitoring, and recording. The system has been designed to include in situ, NIST-traceable calibration. Certain sample tubing retains sufficient water that even if the sample is dry, the sample tube will desorb water to an amount resulting in moisture concentration errors up to 500 ppm for as long as 10 minutes. It was determined that Bev-A-Line IV was the best sample line to use. As a result of this issue, it is prudent to add a high-level humidity sensor to PDP-MS so such events can be prevented in the future.

  8. Real-time Sample Analysis using Sampling Probe and Miniature Mass Spectrometer

    PubMed Central

    Chen, Chien-Hsun; Lin, Ziqing; Tian, Ran; Shi, Riyi; Cooks, R. Graham; Ouyang, Zheng

    2016-01-01

    A miniature mass spectrometry system with a sampling probe has been developed for real-time analysis of chemicals from sample surfaces. The sampling probe is 1.5m in length and is comprised of one channel for introducing the spray and the other channel for transferring the charged species back to the Mini MS. This system provides a solution to the problem of real-time mass spectrometry analysis of a three-dimensional object in the field and is successful with compounds including those in inks, agrochemicals, explosives, and animal tissues. This system can be implemented in the form of a backpack MS with a sampling probe for forensic analysis or in the form of a compact MS with an intra-surgical probe for tissue analysis. PMID:26237577

  9. Real-time measurement of sodium chloride in individual aerosol particles by mass spectrometry

    NASA Technical Reports Server (NTRS)

    Sinha, M. P.; Friedlander, S. K.

    1985-01-01

    The method of particle analysis by mass spectrometry has been applied to the quantitative measurement of sodium chloride in individual particles on a real-time basis. Particles of known masses are individually introduced, in the form of a beam, into a miniature Knudsen cell oven (1600 K). The oven is fabricated from rhenium metal sheet (0.018 mm thick) and is situated in the ion source of a quadrupole mass spectrometer. A particle once inside the oven is trapped and completely volatilized; this overcomes the problem of partial volatilization due to particles bouncing from the filament surface. Individual particles are thermally volatilized and ionized inside the rhenium oven, and produce discrete sodium ion pulses whose intensities are measured with the quadrupole mass spectrometer. An ion pulse width of several milliseconds (4-12 ms) is found for particles in the mass range 1.3 x 10 to the -13th to 5.4 x 10 to the -11th g. The sodium ion intensity is found to be proportional to the particle mass to the 0.86-power. The intensity distribution for monodisperse aerosol particles possesses a geometric standard deviation of 1.09, showing that the method can be used for the determination of the mass distribution function with good resolution in a polydisperse aerosol.

  10. Analysis of writing inks on paper using direct analysis in real time mass spectrometry.

    PubMed

    Jones, Roger W; McClelland, John F

    2013-09-10

    Ink analysis is central to questioned document examination. We applied direct analysis in real time mass spectrometry (DART MS) to ballpoint, gel, and fluid writing ink analysis. DART MS acquires the mass spectrum of an ink while it is still on a document without altering the appearance of the document. Spectra were acquired from ink on a variety of papers, and the spectrum of the blank paper could be subtracted out to produce a cleanly isolated ink spectrum in most cases. Only certain heavy or heavily processed papers interfered. The time since an ink is written on paper has a large effect on its spectrum. DART spectra change radically during the first few months after an ink is written as the more volatile components evaporate, but the spectra stabilize after that. A library-search study involving 166 well-aged inks assessed the ability to identify inks from their DART spectra. The aggregate success rate was 92%.

  11. In Vivo and Real-time Monitoring of Secondary Metabolites of Living Organisms by Mass Spectrometry

    NASA Astrophysics Data System (ADS)

    Hu, Bin; Wang, Lei; Ye, Wen-Cai; Yao, Zhong-Ping

    2013-07-01

    Secondary metabolites are compounds that are important for the survival and propagation of animals and plants. Our current understanding on the roles and secretion mechanism of secondary metabolites is limited by the existing techniques that typically cannot provide transient and dynamic information about the metabolic processes. In this manuscript, by detecting venoms secreted by living scorpion and toad upon attack and variation of alkaloids in living Catharanthus roseus upon stimulation, which represent three different sampling methods for living organisms, we demonstrated that in vivo and real-time monitoring of secondary metabolites released from living animals and plants could be readily achieved by using field-induced direct ionization mass spectrometry.

  12. Ricin Activity Assay by Direct Analysis in Real Time Mass Spectrometry Detection of Adenine Release

    DTIC Science & Technology

    2010-02-01

    direct analysis in real time mass spectrometry. The release of adenine from the inhomo- geneous substrate herring sperm DNA by ricin was determined to...chain catalyzes cleavage at adenosine 4324 (in rat RNA) of 28S rRNA to release adenine.10 This action inhibits protein synthesis, leading to cell...death. In addition to RNA, herring sperm DNA (hsDNA) is a substrate for ricin.11 We chose to employ hsDNA for this assay because it is relatively stable

  13. Letter: High-mass capabilities of positive-ion and negative-ion direct analysis in real time mass spectrometry.

    PubMed

    Gross, Jürgen H

    2016-01-01

    Of the ionic liquid 1-butyl-3-methylimidazolium (C(+)) tricyanomethide (A(-)) high-mass cluster ions of both positive ([C(n)A(n-1)](+)) and negative ([C(n-1)A(n)](-)) charge were generated and detected by direct analysis in real time (DART) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS). After optimization of the settings of the DART ionization source and of the mass analyzer ions of m/z values unprecedented in DART-MS were detected. Thus, the upper m/z limits of positive-ion and negative-ion DART- MS were substantially expanded. Negative-ion DART-MS delivered cluster ions up to [C(15)A(16)](-), m/z 3527 (nominal mass of monoisotopic ion), while positive-ion DART-MS even yielded ions up to [C(30)A(29)](+), m/z 6784. The identification of the cluster ions is supported by their accurate mass and exact mass differences corresponding to CA between adjacent cluster ion peaks.

  14. Real-Time Cellular Exometabolome Analysis with a Microfluidic-Mass Spectrometry Platform

    PubMed Central

    Marasco, Christina C.; Enders, Jeffrey R.; Seale, Kevin T.; McLean, John A.; Wikswo, John P.

    2015-01-01

    To address the challenges of tracking the multitude of signaling molecules and metabolites that is the basis of biological complexity, we describe a strategy to expand the analytical techniques for dynamic systems biology. Using microfluidics, online desalting, and mass spectrometry technologies, we constructed and validated a platform well suited for sampling the cellular microenvironment with high temporal resolution. Our platform achieves success in: automated cellular stimulation and microenvironment control; reduced non-specific adsorption to polydimethylsiloxane due to surface passivation; real-time online sample collection; near real-time sample preparation for salt removal; and real-time online mass spectrometry. When compared against the benchmark of “in-culture” experiments combined with ultraperformance liquid chromatography-electrospray ionization-ion mobility-mass spectrometry (UPLC-ESI-IM-MS), our platform alleviates the volume challenge issues caused by dilution of autocrine and paracrine signaling and dramatically reduces sample preparation and data collection time, while reducing undesirable external influence from various manual methods of manipulating cells and media (e.g., cell centrifugation). To validate this system biologically, we focused on cellular responses of Jurkat T cells to microenvironmental stimuli. Application of these stimuli, in conjunction with the cell’s metabolic processes, results in changes in consumption of nutrients and secretion of biomolecules (collectively, the exometabolome), which enable communication with other cells or tissues and elimination of waste. Naïve and experienced T-cell metabolism of cocaine is used as an exemplary system to confirm the platform’s capability, highlight its potential for metabolite discovery applications, and explore immunological memory of T-cell drug exposure. Our platform proved capable of detecting metabolomic variations between naïve and experienced Jurkat T cells and

  15. Globally referenced real time monitoring of mass movements using monoscopic time-lapse photography.

    NASA Astrophysics Data System (ADS)

    Kenner, Robert; Phillips, Marcia; Buchroithner, Manfred

    2016-04-01

    The creep movement of a rock glacier was monitored in daily resolution using images of an automatic in-situ time-lapse camera (AC). Displacements were calculated between the images in 2D image coordinates using the imaging velocimetry algorithm of Roesgen and Totaro, 1995. To georeference and scale these displacements, a creep velocity field captured once by a terrestrial laser scan (TLS) repeat measurement was used. The laser scan point cloud and the creep velocity vector field were projected in image coordinates of the AC to obtain a georeferencing mask, a scale mask and an azimuth mask for the 2D displacements calculated between two images. The scale mask was obtained by comparing the TLS derived displacement vectors with those of the AC, referring to a common measurement period. The automatic procedure includes the following work steps: 1. Offsets between two images are identified and corrected based on image parts representing unchanged terrain. 2. 2D displacements are calculated between all chronological image sequences. 3. Faulty displacement vectors are eliminated based on a predefined threshold for spatial direction differences. 4. The remaining displacements are georeferenced, scaled and attributed with individual displacement directions (azimuths) in global coordinates. 5. In addition to the displacement values, displacement velocities and accelerations are calculated using the date of the images. 6. For chronologically successive displacement vector fields, the spatial mean of the relative velocity is defined and expressed as a percentage of the first displacement velocity in the series. The time series of the relative velocities is expressed in chart form. 7. The spatial resolution of all georeferenced output data sets is homogenized, as they were influenced by the central projection of the photos. The described procedure proved to be a reliable, low cost method to monitor mass wasting processes. Even under difficult conditions, like thin snow coverage

  16. Real-time Ensemble Forecasting of Coronal Mass Ejections using the WSA-ENLIL+Cone Model

    NASA Astrophysics Data System (ADS)

    Mays, M. L.; Taktakishvili, A.; Pulkkinen, A. A.; MacNeice, P. J.; Rastaetter, L.; Kuznetsova, M. M.; Odstrcil, D.

    2013-12-01

    Ensemble forecasting of coronal mass ejections (CMEs) provides significant information in that it provides an estimation of the spread or uncertainty in CME arrival time predictions due to uncertainties in determining CME input parameters. Ensemble modeling of CME propagation in the heliosphere is performed by forecasters at the Space Weather Research Center (SWRC) using the WSA-ENLIL cone model available at the Community Coordinated Modeling Center (CCMC). SWRC is an in-house research-based operations team at the CCMC which provides interplanetary space weather forecasting for NASA's robotic missions and performs real-time model validation. A distribution of n (routinely n=48) CME input parameters are generated using the CCMC Stereo CME Analysis Tool (StereoCAT) which employs geometrical triangulation techniques. These input parameters are used to perform n different simulations yielding an ensemble of solar wind parameters at various locations of interest (satellites or planets), including a probability distribution of CME shock arrival times (for hits), and geomagnetic storm strength (for Earth-directed hits). Ensemble simulations have been performed experimentally in real-time at the CCMC since January 2013. We present the results of ensemble simulations for a total of 15 CME events, 10 of which were performed in real-time. The observed CME arrival was within the range of ensemble arrival time predictions for 5 out of the 12 ensemble runs containing hits. The average arrival time prediction was computed for each of the twelve ensembles predicting hits and using the actual arrival time an average absolute error of 8.20 hours was found for all twelve ensembles, which is comparable to current forecasting errors. Some considerations for the accuracy of ensemble CME arrival time predictions include the importance of the initial distribution of CME input parameters, particularly the mean and spread. When the observed arrivals are not within the predicted range, this

  17. Real-time monitoring of enzymatic DNA hydrolysis by electrospray ionization mass spectrometry.

    PubMed

    van den Heuvel, Robert H H; Gato, Sara; Versluis, Cees; Gerbaux, Pascal; Kleanthous, Colin; Heck, Albert J R

    2005-06-13

    A fast and direct method for the monitoring of enzymatic DNA hydrolysis was developed using electrospray ionization mass spectrometry. We incorporated the use of a robotic chip-based electrospray ionization source for increased reproducibility and throughput. The mass spectrometry method allows the detection of DNA fragments and intact non-covalent protein-DNA complexes in a single experiment. We used the method to monitor in real-time single-stranded (ss) DNA hydrolysis by colicin E9 DNase and to characterize transient non-covalent E9 DNase-DNA complexes present during the hydrolysis reaction. The mass spectra showed that E9 DNase interacts with ssDNA in the absence of a divalent metal ion, but is strictly dependent on Ni2+ or Co2+ for ssDNA hydrolysis. We demonstrated that the sequence selectivity of E9 DNase is dependent on the ratio protein:ssDNA or the ssDNA concentration and that only 3'-hydroxy and 5'-phosphate termini are produced. It was also shown that the homologous E7 DNase is reactive with Zn2+ as transition metal ion and that this DNase displays a different sequence selectivity. The method described is of general use to analyze the reactivity and specificity of nucleases.

  18. Quality by design study of the direct analysis in real time mass spectrometry response.

    PubMed

    Wang, Lu; Chen, Teng; Zeng, Shanshan; Qu, Haibin

    2014-02-01

    A mass spectrometry method has been developed using the Quality by Design (QbD) principle. Direct analysis in real time mass spectrometry (DART-MS) was adopted to analyze a pharmaceutical preparation. A fishbone diagram for DART-MS and the Plackett-Burman design were utilized to evaluate the impact of a number of factors on the method performance. Multivariate regression and Pareto ranking analysis indicated that the temperature, determined distance, and sampler speed were statistically significant (P < 0.05). Furthermore, the Box-Behnken design combined with response surface analysis was then employed to study the relationships between these three factors and the quality of the DART-MS analysis. The analytical design space of DART-MS was thus constructed and its robustness was validated. In this presented approach, method performance was mathematically described as a composite desirability function of the critical quality attributes (CQAs). Two terms of method validation, including analytical repeatability and method robustness, were carried out at an operating work point. Finally, the validated method was successfully applied to the pharmaceutical quality assurance in different manufacturing batches. These results revealed that the QbD concept was practical in DART-MS method development. Meanwhile, the determined quality was controlled by the analytical design space. This presented strategy provided a tutorial to the development of a robust QbD-compliant mass spectrometry method for industrial quality control.

  19. Quality by Design Study of the Direct Analysis in Real Time Mass Spectrometry Response

    NASA Astrophysics Data System (ADS)

    Wang, Lu; Chen, Teng; Zeng, Shanshan; Qu, Haibin

    2013-12-01

    A mass spectrometry method has been developed using the Quality by Design (QbD) principle. Direct analysis in real time mass spectrometry (DART-MS) was adopted to analyze a pharmaceutical preparation. A fishbone diagram for DART-MS and the Plackett-Burman design were utilized to evaluate the impact of a number of factors on the method performance. Multivariate regression and Pareto ranking analysis indicated that the temperature, determined distance, and sampler speed were statistically significant (P < 0.05). Furthermore, the Box-Behnken design combined with response surface analysis was then employed to study the relationships between these three factors and the quality of the DART-MS analysis. The analytical design space of DART-MS was thus constructed and its robustness was validated. In this presented approach, method performance was mathematically described as a composite desirability function of the critical quality attributes (CQAs). Two terms of method validation, including analytical repeatability and method robustness, were carried out at an operating work point. Finally, the validated method was successfully applied to the pharmaceutical quality assurance in different manufacturing batches. These results revealed that the QbD concept was practical in DART-MS method development. Meanwhile, the determined quality was controlled by the analytical design space. This presented strategy provided a tutorial to the development of a robust QbD-compliant mass spectrometry method for industrial quality control.

  20. Real-Time Broad Spectrum Characterization of Hazardous Mixed Waste by Membrane Introduction Mass Spectrometry

    SciTech Connect

    Wilkerson Jr., Charles W.

    2000-12-31

    The goal of this project was to expand the range of chemical species that may be detected by membrane introduction mass spectrometry (MIMS) in environmental, and specifically in Mixed Waste, monitoring and characterization applications. Membrane introduction mass spectrometry (MIMS) functions as a near real-time monitor: there is little to no sample preparation and t analysis time is seconds to minutes. MIMS can be implemented as a flow injection technique, where samples, standards, and method blanks can be sequentially analyzed in a continuous fashion. The membrane acts as an interface between the sample (air or water) and the vacuum of the mass spectrometer. Transport of the analyte through the membrane occurs by the process of pervaporation. This process is described by adsorption to the outer surface of the membrane, diffusion through the membrane, and desorption from the inner membrane surface into a helium gas flow or into vacuum. The driving force for this work is the need for a rapid, sensitive, and broadly applicable tool for characterizing organic and metal-containing contaminants in a variety of DOE (and other) waste streams. In all characterization scenarios, a balance must be struck between evaluation of the hazards and their extent at a waste site, and the resources available for the overall mitigation of that risk. In the case of chemically, physically, and geometrically homogeneous waste, the situation is aided by the ability to reasonably assume that any sample collected is representative of the overall site constituents. However, few real environmental challenges are homogeneous. As a result, detailed sampling plans must be prepared, and chemical analyses must be performed on a number of samples in order to identify areas of contamination and assess further options. For many years, the chemical analysis part of this process has been accomplished by delivering the samples to a (typically) physically remote laboratory, where very detailed, and

  1. Characterization of individual fine and ultrafine particles with a real-time single particle mass spectrometer

    NASA Astrophysics Data System (ADS)

    Reinard, Melissa S.

    2008-10-01

    Designed to analyze aerosols in ambient settings, the Real-Time Single Particle Mass Spectrometer (RSMS) provides a highly time-resolved measurement of the physical and chemical properties of individual fine (<2.5 mum dia.) and ultrafine (<0.1 mum dia.) particles. Understanding aerosols within this size range is crucial as these particles greatly impact both human health and the environment. Data collected by RSMS can be used identify particle sources and atmospheric processes. RSMS was deployed to Wilmington, DE during 2005-2006 as part of E-DATAS (Enhanced Delaware Air Toxics Assessment Study), a collaboration with the Delaware Department of Natural Resources (DNREC), the Environmental Protection Agency (EPA) and Duke University. Mass spectra acquired by RSMS were compared to a Scanning Mobility Particle Sizer (SMPS) to give a quantitative estimate of the chemical composition of PM1.0 (particulate matter <1.0 mum dia.) impacting the city. A method to collect and analyze particles directly from emission stacks of industrial facilities was developed to help identify sources of PM. Single particle mass spectrometry, in general, has remained primarily a qualitative technique due to several instrumental limitations which affect the data. First, the shot-to-shot variation in the laser pulse caused inconsistencies between the mass spectra of particles with the same composition. To determine whether this variation was systematic or random, the covariance between ions was calculated for laboratory generated aerosols. Second, RSMS was found to be highly sensitive to specific chemical species such as ammonium nitrate and transition/alkali metals. When these compounds are present in a particle they dominate the mass spectra and dwarf other the signal from other components to the baseline. To explore this bias, data collected by RSMS in Wilmington, DE was compared to data also collected in Wilmington by the quantitative NanoAerosol Mass Spectrometer (NAMS). Finally, a light

  2. Demonstration of real-time monitoring of a photolithographic exposure process using chemical ionization mass spectrometry

    SciTech Connect

    Mowry, C.D.

    1998-02-01

    Silicon wafers are coated with photoresist and exposed to ultraviolet (UV) light in a laboratory to simulate typical conditions expected in an actual semiconductor manufacturing process tool. Air is drawn through the exposure chamber and analyzed using chemical ionization mass spectrometry (CI/MS). Species that evaporate or outgas from the wafer are thus detected. The purpose of such analyses is to determine the potential of CI/MS as a real-time process monitoring tool. Results demonstrate that CI/MS can remotely detect the products evolved before, during, and after wafer UV exposure; and that the quantity and type of products vary with the photoresist coated on the wafer. Such monitoring could provide semiconductor manufacturers benefits in quality control and process analysis. Tool and photoresist manufacturers could also realize benefits from this measurement technique with respect to new tool, method, or photoresist development. The benefits realized can lead to improved device yields and reduced product and development costs.

  3. Real-time detection of airborne viruses on a mass-sensitive device

    PubMed Central

    Lee, Joonhyung; Jang, Jaesung; Akin, Demir; Savran, Cagri A.; Bashir, Rashid

    2008-01-01

    We present real-time detection of airborne Vaccinia viruses using quartz crystal microbalance (QCM) in an integrated manner. Vaccinia viruses were aerosolized and neutralized using an electrospray aerosol generator, transported into the QCM chamber, and captured by a QCM crystal. The capture of the viruses on the QCM crystal resulted in frequency shifts proportional to the number of viruses. The capture rate varied linearly with the concentration of initial virus suspensions (8.5×108–8.5×1010 particles∕ml) at flow rates of 2.0 and 1.1 l∕min. This work demonstrates the general potential of mass sensitive detection of nanoscale biological entities in air. PMID:19529841

  4. Profiling of Piper betle Linn. cultivars by direct analysis in real time mass spectrometric technique.

    PubMed

    Bajpai, Vikas; Sharma, Deepty; Kumar, Brijesh; Madhusudanan, K P

    2010-12-01

    Piper betle Linn. is a traditional plant associated with the Asian and southeast Asian cultures. Its use is also recorded in folk medicines in these regions. Several of its medicinal properties have recently been proven. Phytochemical analysis showed the presence of mainly terpenes and phenols in betel leaves. These constituents vary in the different cultivars of Piper betle. In this paper we have attempted to profile eight locally available betel cultivars using the recently developed mass spectral ionization technique of direct analysis in real time (DART). Principal component analysis has also been employed to analyze the DART MS data of these betel cultivars. The results show that the cultivars of Piper betle could be differentiated using DART MS data.

  5. Advanced Automation for Ion Trap Mass Spectrometry-New Opportunities for Real-Time Autonomous Analysis

    NASA Technical Reports Server (NTRS)

    Palmer, Peter T.; Wong, C. M.; Salmonson, J. D.; Yost, R. A.; Griffin, T. P.; Yates, N. A.; Lawless, James G. (Technical Monitor)

    1994-01-01

    The utility of MS/MS for both target compound analysis and the structure elucidation of unknowns has been described in a number of references. A broader acceptance of this technique has not yet been realized as it requires large, complex, and costly instrumentation which has not been competitive with more conventional techniques. Recent advancements in ion trap mass spectrometry promise to change this situation. Although the ion trap's small size, sensitivity, and ability to perform multiple stages of mass spectrometry have made it eminently suitable for on-line, real-time monitoring applications, advance automation techniques are required to make these capabilities more accessible to non-experts. Towards this end we have developed custom software for the design and implementation of MS/MS experiments. This software allows the user to take full advantage of the ion trap's versatility with respect to ionization techniques, scan proxies, and ion accumulation/ejection methods. Additionally, expert system software has been developed for autonomous target compound analysis. This software has been linked to ion trap control software and a commercial data system to bring all of the steps in the analysis cycle under control of the expert system. These software development efforts and their utilization for a number of trace analysis applications will be described.

  6. Direct analysis in real time mass spectrometry (DART-MS) of "bath salt" cathinone drug mixtures.

    PubMed

    Lesiak, Ashton D; Musah, Rabi A; Cody, Robert B; Domin, Marek A; Dane, A John; Shepard, Jason R E

    2013-06-21

    Rapid and versatile direct analysis in real time mass spectrometry (DART-MS) methods were developed for detection and characterization of synthetic cathinone designer drugs, also known as "bath salts". The speed and efficiency associated with DART-MS testing of such highly unpredictable samples demonstrate the technique as an attractive alternative to conventional GC-MS and LC-MS methods. A series of isobaric and closely related synthetic cathinones, alone and in mixtures, were differentiated using high mass accuracy and in-source collision induced dissociation (CID). Crime laboratories have observed a dramatic rise in the use of these substances, which has caused sample testing backlogs, particularly since the myriad of structurally related compounds are challenging to efficiently differentiate. This challenge is compounded by the perpetual emergence of new structural variants as soon as older generation derivatives become scheduled. Because of the numerous chemical substances that fall into these categories, along with the varying composition and complexity of mixtures of these drugs, DART-MS CID has the potential to dramatically streamline sample analysis, minimize the number of sample preparation steps, and enable rapid characterization of emerging structural analogs.

  7. Direct analysis in real time-Mass spectrometry (DART-MS) in forensic and security applications.

    PubMed

    Pavlovich, Matthew J; Musselman, Brian; Hall, Adam B

    2016-06-06

    Over the last decade, direct analysis in real time (DART) has emerged as a viable method for fast, easy, and reliable "ambient ionization" for forensic analysis. The ability of DART to generate ions from chemicals that might be present at the scene of a criminal activity, whether they are in the gas, liquid, or solid phase, with limited sample preparation has made the technology a useful analytical tool in numerous forensic applications. This review paper summarizes many of those applications, ranging from the analysis of trace evidence to security applications, with a focus on providing the forensic scientist with a resource for developing their own applications. The most common uses for DART in forensics are in studying seized drugs, drugs of abuse and their metabolites, bulk and detonated explosives, toxic chemicals, chemical warfare agents, inks and dyes, and commercial plant and animal products that have been adulterated for economic gain. This review is meant to complement recent reviews that have described the fundamentals of the ionization mechanism and the general use of DART. We describe a wide range of forensic applications beyond the field of analyzing drugs of abuse, which dominates the literature, including common experimental and data analysis methods. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 9999: XX-XX, 2016.

  8. MALDI mass spectrometry imaging analysis of pituitary adenomas for near-real-time tumor delineation

    PubMed Central

    Calligaris, David; Feldman, Daniel R.; Norton, Isaiah; Olubiyi, Olutayo; Changelian, Armen N.; Machaidze, Revaz; Vestal, Matthew L.; Laws, Edward R.; Dunn, Ian F.; Santagata, Sandro; Agar, Nathalie Y. R.

    2015-01-01

    We present a proof of concept study designed to support the clinical development of mass spectrometry imaging (MSI) for the detection of pituitary tumors during surgery. We analyzed by matrix-assisted laser desorption/ionization (MALDI) MSI six nonpathological (NP) human pituitary glands and 45 hormone secreting and nonsecreting (NS) human pituitary adenomas. We show that the distribution of pituitary hormones such as prolactin (PRL), growth hormone (GH), adrenocorticotropic hormone (ACTH), and thyroid stimulating hormone (TSH) in both normal and tumor tissues can be assessed by using this approach. The presence of most of the pituitary hormones was confirmed by using MS/MS and pseudo-MS/MS methods, and subtyping of pituitary adenomas was performed by using principal component analysis (PCA) and support vector machine (SVM). Our proof of concept study demonstrates that MALDI MSI could be used to directly detect excessive hormonal production from functional pituitary adenomas and generally classify pituitary adenomas by using statistical and machine learning analyses. The tissue characterization can be completed in fewer than 30 min and could therefore be applied for the near-real-time detection and delineation of pituitary tumors for intraoperative surgical decision-making. PMID:26216958

  9. Monitoring Enzymatic Reactions in Real Time Using Venturi Easy Ambient Sonic-Spray Ionization Mass Spectrometry

    PubMed Central

    2016-01-01

    We developed a technique to monitor spatially confined surface reactions with mass spectrometry under ambient conditions, without the need for voltage or organic solvents. Fused-silica capillaries immersed in an aqueous solution, positioned in close proximity to each other and the functionalized surface, created a laminar flow junction with a resulting reaction volume of ∼5 pL. The setup was operated with a syringe pump, delivering reagents to the surface through a fused-silica capillary. The other fused-silica capillary was connected to a Venturi easy ambient sonic-spray ionization source, sampling the resulting analytes at a slightly higher flow rate compared to the feeding capillary. The combined effects of the inflow and outflow maintains a chemical microenvironment, where the rate of advective transport overcomes diffusion. We show proof-of-concept where acetylcholinesterase was immobilized on an organosiloxane polymer through electrostatic interactions. The hydrolysis of acetylcholine by acetylcholinesterase into choline was monitored in real-time for a range of acetylcholine concentrations, fused-silica capillary geometries, and operating flow rates. Higher reaction rates and conversion yields were observed with increasing acetylcholine concentrations, as would be expected. PMID:27249533

  10. Monitoring Enzymatic Reactions in Real Time Using Venturi Easy Ambient Sonic-Spray Ionization Mass Spectrometry.

    PubMed

    Jansson, Erik T; Dulay, Maria T; Zare, Richard N

    2016-06-21

    We developed a technique to monitor spatially confined surface reactions with mass spectrometry under ambient conditions, without the need for voltage or organic solvents. Fused-silica capillaries immersed in an aqueous solution, positioned in close proximity to each other and the functionalized surface, created a laminar flow junction with a resulting reaction volume of ∼5 pL. The setup was operated with a syringe pump, delivering reagents to the surface through a fused-silica capillary. The other fused-silica capillary was connected to a Venturi easy ambient sonic-spray ionization source, sampling the resulting analytes at a slightly higher flow rate compared to the feeding capillary. The combined effects of the inflow and outflow maintains a chemical microenvironment, where the rate of advective transport overcomes diffusion. We show proof-of-concept where acetylcholinesterase was immobilized on an organosiloxane polymer through electrostatic interactions. The hydrolysis of acetylcholine by acetylcholinesterase into choline was monitored in real-time for a range of acetylcholine concentrations, fused-silica capillary geometries, and operating flow rates. Higher reaction rates and conversion yields were observed with increasing acetylcholine concentrations, as would be expected.

  11. Characterizing and classifying water-based lubricants using direct analysis in real time(®)-time of flight mass spectrometry.

    PubMed

    Maric, Mark; Bridge, Candice

    2016-09-01

    Lubricant analysis is a relatively recent addition to the examination protocol in sexual assault cases by the forensic science community. Currently, lubricants cannot be unequivocally identified, although their presence can be determined based on the detection of a few chemical components, i.e. polydimethylsiloxane, polyethylene glycol, glycerol or nonoxynol-9. Confirmation of their presence typically requires that an authentic reference sample be submitted and compared to the unknown sample to determine if they potentially came from the same source. In this study, 33 individual personal water-based lubricants were characterized by direct analysis in real time-time of flight mass spectroscopy (DART-TOFMS). The resultant mass spectral data were evaluated using well-established multivariate statistical techniques, such as principal component and linear discriminant analysis. Statistical analysis revealed six different groupings within the data that could be correlated to sub-categories of water-based lubricants that contain additives in the form of anesthetics, sensation enhancers and flavorings. This variability in the personal lubricant sources can be utilized to aid in identifying the specific type of lubricant when only a questioned sample is available.

  12. Determination of left ventricular volume, ejection fraction, and myocardial mass by real-time three-dimensional echocardiography

    NASA Technical Reports Server (NTRS)

    Qin, J. X.; Shiota, T.; Thomas, J. D.

    2000-01-01

    Reconstructed three-dimensional (3-D) echocardiography is an accurate and reproducible method of assessing left ventricular (LV) functions. However, it has limitations for clinical study due to the requirement of complex computer and echocardiographic analysis systems, electrocardiographic/respiratory gating, and prolonged imaging times. Real-time 3-D echocardiography has a major advantage of conveniently visualizing the entire cardiac anatomy in three dimensions and of potentially accurately quantifying LV volumes, ejection fractions, and myocardial mass in patients even in the presence of an LV aneurysm. Although the image quality of the current real-time 3-D echocardiographic methods is not optimal, its widespread clinical application is possible because of the convenient and fast image acquisition. We review real-time 3-D echocardiographic image acquisition and quantitative analysis for the evaluation of LV function and LV mass.

  13. Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

    PubMed Central

    Lesiak, Ashton D.; Musah, Rabi A.

    2016-01-01

    We demonstrate that direct analysis in real time-high resolution mass spectrometry can be used to produce mass spectral profiles of botanical material, and that these chemical fingerprints can be used for plant species identification. The mass spectral data can be acquired rapidly and in a high throughput manner without the need for sample extraction, derivatization or pH adjustment steps. The use of this technique bypasses challenges presented by more conventional techniques including lengthy chromatography analysis times and resource intensive methods. The high throughput capabilities of the direct analysis in real time-high resolution mass spectrometry protocol, coupled with multivariate statistical analysis processing of the data, provide not only class characterization of plants, but also yield species and varietal information. Here, the technique is demonstrated with two psychoactive plant products, Mitragyna speciosa (Kratom) and Datura (Jimsonweed), which were subjected to direct analysis in real time-high resolution mass spectrometry followed by statistical analysis processing of the mass spectral data. The application of these tools in tandem enabled the plant materials to be rapidly identified at the level of variety and species. PMID:27768072

  14. Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry.

    PubMed

    Lesiak, Ashton D; Musah, Rabi A

    2016-10-02

    We demonstrate that direct analysis in real time-high resolution mass spectrometry can be used to produce mass spectral profiles of botanical material, and that these chemical fingerprints can be used for plant species identification. The mass spectral data can be acquired rapidly and in a high throughput manner without the need for sample extraction, derivatization or pH adjustment steps. The use of this technique bypasses challenges presented by more conventional techniques including lengthy chromatography analysis times and resource intensive methods. The high throughput capabilities of the direct analysis in real time-high resolution mass spectrometry protocol, coupled with multivariate statistical analysis processing of the data, provide not only class characterization of plants, but also yield species and varietal information. Here, the technique is demonstrated with two psychoactive plant products, Mitragyna speciosa (Kratom) and Datura (Jimsonweed), which were subjected to direct analysis in real time-high resolution mass spectrometry followed by statistical analysis processing of the mass spectral data. The application of these tools in tandem enabled the plant materials to be rapidly identified at the level of variety and species.

  15. Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry.

    PubMed

    Bandura, Dmitry R; Baranov, Vladimir I; Ornatsky, Olga I; Antonov, Alexei; Kinach, Robert; Lou, Xudong; Pavlov, Serguei; Vorobiev, Sergey; Dick, John E; Tanner, Scott D

    2009-08-15

    A novel instrument for real time analysis of individual biological cells or other microparticles is described. The instrument is based on inductively coupled plasma time-of-flight mass spectrometry and comprises a three-aperture plasma-vacuum interface, a dc quadrupole turning optics for decoupling ions from neutral components, an rf quadrupole ion guide discriminating against low-mass dominant plasma ions, a point-to-parallel focusing dc quadrupole doublet, an orthogonal acceleration reflectron analyzer, a discrete dynode fast ion detector, and an 8-bit 1 GHz digitizer. A high spectrum generation frequency of 76.8 kHz provides capability for collecting multiple spectra from each particle-induced transient ion cloud, typically of 200-300 micros duration. It is shown that the transients can be resolved and characterized individually at a peak frequency of 1100 particles per second. Design considerations and optimization data are presented. The figures of merit of the instrument are measured under standard inductively coupled plasma (ICP) operating conditions (<3% cerium oxide ratio). At mass resolution (full width at half-maximum) M/DeltaM > 900 for m/z = 159, the sensitivity with a standard sample introduction system of >1.4 x 10(8) ion counts per second per mg L(-1) of Tb and an abundance sensitivity of (6 x 10(-4))-(1.4 x 10(-3)) (trailing and leading masses, respectively) are shown. The mass range (m/z = 125-215) and abundance sensitivity are sufficient for elemental immunoassay with up to 60 distinct available elemental tags. When <15 elemental tags are used, a higher sensitivity mode at lower resolution (M/DeltaM > 500) can be used, which provides >2.4 x 10(8) cps per mg L(-1) of Tb, at (1.5 x 10(-3))-(5.0 x 10(-3)) abundance sensitivity. The real-time simultaneous detection of multiple isotopes from individual 1.8 microm polystyrene beads labeled with lanthanides is shown. A real time single cell 20 antigen expression assay of model cell lines and leukemia

  16. Effect of Body Mass Index on Intrafraction Prostate Displacement Monitored by Real-Time Electromagnetic Tracking

    SciTech Connect

    Butler, Wayne M.; Morris, Mallory N.; Merrick, Gregory S.; Kurko, Brian S.; Murray, Brian C.

    2012-10-01

    Purpose: To evaluate, using real-time monitoring of implanted radiofrequency transponders, the intrafraction prostate displacement of patients as a function of body mass index (BMI). Methods and Materials: The motions of Beacon radiofrequency transponders (Calypso Medical Technologies, Seattle, WA) implanted in the prostate glands of 66 men were monitored throughout the course of intensity modulated radiation therapy. Data were acquired at 10 Hz from setup to the end of treatment, but only the 1.7 million data points with a 'beam on' tag were used in the analysis. There were 21 obese patients, with BMI {>=}30 and 45 nonobese patients in the study. Results: Mean displacements were least in the left-right lateral direction (0.56 {+-} 0.24 mm) and approximately twice that magnitude in the superior-inferior and anterior-posterior directions. The net vector displacement was larger still, 1.95 {+-} 0.47 mm. Stratified by BMI cohort, the mean displacements per patient in the 3 Cartesian axes as well as the net vector for patients with BMI {>=}30 were slightly less (<0.2 mm) but not significantly different than the corresponding values for patients with lower BMIs. As a surrogate for the magnitude of oscillatory noise, the standard deviation for displacements in all measured planes showed no significant differences in the prostate positional variability between the lower and higher BMI groups. Histograms of prostate displacements showed a lower frequency of large displacements in obese patients, and there were no significant differences in short-term and long-term velocity distributions. Conclusions: After patients were positioned accurately using implanted radiofrequency transponders, the intrafractional displacements in the lateral, superior-inferior, and anterior-posterior directions as well as the net vector displacements were smaller, but not significantly so, for obese men than for those with lower BMI.

  17. Determination of Dicyandiamide in Powdered Milk Using Direct Analysis in Real Time Quadrupole Time-of-Flight Tandem Mass Spectrometry

    NASA Astrophysics Data System (ADS)

    Zhang, Liya; Yong, Wei; Liu, Jiahui; Wang, Sai; Chen, Qilong; Guo, Tianyang; Zhang, Jichuan; Tan, Tianwei; Su, Haijia; Dong, Yiyang

    2015-08-01

    The direct analysis in real time (DART) ionization source coupled with quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) system has the capability to desorb analytes directly from samples without sample cleanup or chromatographic separation. In this work, a method based on DART/Q-TOF MS/MS has been developed for rapid identification of dicyandiamide (DCD) present in powdered milk. Simple sample extraction procedure employing acetonitrile-water (80:20, v/v) mixture was followed by direct, high-throughput determination of sample extracts spread on a steel mesh of the transmission module by mass spectrometry under ambient conditions. The method has been evaluated for both qualitative and quantitative analysis of DCD in powdered milk. Variables including experimental apparatus, DART gas heater temperature, sample presentation speed, and vacuum pressure were investigated. The quantitative method was validated with respect to linearity, sensitivity, repeatability, precision, and accuracy by using external standards. After optimization of these parameters, a limit of detection (LOD) of 100 μg kg-1 was obtained for DCD with a linear working range from 100 to 10000 μg kg-1 and a satisfactory correlation coefficient (R2) of 0.9997. Good recovery (80.08%-106.47%) and repeatability (RSD = 3.0%-5.4%) were achieved for DCD. The DART/Q-TOF MS/MS-based method provides a rapid, efficient, and powerful scheme to analyze DCD in powdered milk with limited sample preparation, thus reducing time and complexity of quality control.

  18. Determination of Dicyandiamide in Powdered Milk Using Direct Analysis in Real Time Quadrupole Time-of-Flight Tandem Mass Spectrometry.

    PubMed

    Zhang, Liya; Yong, Wei; Liu, Jiahui; Wang, Sai; Chen, Qilong; Guo, Tianyang; Zhang, Jichuan; Tan, Tianwei; Su, Haijia; Dong, Yiyang

    2015-08-01

    The direct analysis in real time (DART) ionization source coupled with quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) system has the capability to desorb analytes directly from samples without sample cleanup or chromatographic separation. In this work, a method based on DART/Q-TOF MS/MS has been developed for rapid identification of dicyandiamide (DCD) present in powdered milk. Simple sample extraction procedure employing acetonitrile-water (80:20, v/v) mixture was followed by direct, high-throughput determination of sample extracts spread on a steel mesh of the transmission module by mass spectrometry under ambient conditions. The method has been evaluated for both qualitative and quantitative analysis of DCD in powdered milk. Variables including experimental apparatus, DART gas heater temperature, sample presentation speed, and vacuum pressure were investigated. The quantitative method was validated with respect to linearity, sensitivity, repeatability, precision, and accuracy by using external standards. After optimization of these parameters, a limit of detection (LOD) of 100 μg kg(-1) was obtained for DCD with a linear working range from 100 to 10000 μg kg(-1) and a satisfactory correlation coefficient (R(2)) of 0.9997. Good recovery (80.08%-106.47%) and repeatability (RSD = 3.0%-5.4%) were achieved for DCD. The DART/Q-TOF MS/MS-based method provides a rapid, efficient, and powerful scheme to analyze DCD in powdered milk with limited sample preparation, thus reducing time and complexity of quality control.

  19. Determination of the aflatoxin AFB1 from corn by direct analysis in real time-mass spectrometry (DART-MS)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Direct analysis in real time (DART) ionization coupled to a high resolution mass spectrometer (MS) was used for screening of aflatoxins from a variety of surfaces and the rapid quantitative analysis of a common form of aflatoxin, AFB1, extracted from corn. Sample preparation procedure and instrument...

  20. Analysis of the aflatoxin AFB1 from corn by direct analysis in real time - mass spectrometry (DART-MS)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Direct analysis in real time (DART) ionization coupled to a high resolution mass spectrometer (MS) was used for screening of aflatoxins from a variety of surfaces and the rapid quantitative analysis of aflatoxins extracted from corn. Sample preparation procedure and instrument parameter settings wer...

  1. Determination of T-2 and HT-2 toxins from maize by direct analysis in real time mass spectrometry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Direct analysis in real time (DART) ionization coupled to mass spectrometry (MS) was used for the rapid quantitative analysis of T-2 toxin, and the related HT-2 toxin, extracted from corn. Sample preparation procedures and instrument parameters were optimized to obtain sensitive and accurate determi...

  2. Detection of illicit drugs on surfaces using direct analysis in real time (DART) time-of-flight mass spectrometry.

    PubMed

    Grange, Andrew H; Sovocool, G Wayne

    2011-05-15

    Methamphetamine (meth) from meth syntheses or habitual meth smoking deposited on household surfaces poses human health hazards. The U.S. State Departments of Health require decontamination of sites where meth was synthesized (meth labs) before they are sold. National Institute for Occupational Safety and Health (NIOSH) methods for meth analysis require wipe sampling, extraction, clean-up, solvent exchange, derivatization, and/or mass spectral analysis using selected ion monitoring. Rapid and inexpensive analyses could screen for drug-contamination within structures with greater spatial resolution, provide real-time analyses during decontamination, and provide thorough documentation of successful clean ups. Herein an autosampler/open-air ion source time-of-flight mass spectrometric technique is described that required only direct sampling using cotton-swab wipes. Each wipe sample collection required 2 min and data acquisition required only 13 s per sample. Optimum collision-induced dissociation voltages, desorption gas temperatures, and wipe sample solvents were determined for 11 drugs. Peaks were observed in analyte-ion traces for 0.025 µg/100 cm(2) of meth and seven other drugs. This level is half the detection limit of NIOSH methods and one-fourth of the lowest U.S. state decontamination limit for meth. Dynamic ranges of 100 in concentration were demonstrated for eight drugs, which is sufficient for a screening technique. The volatilities of 11 drugs deposited on glass were determined. The pick up of the drugs by solvent-soaked cotton-swab wipes from glass relative to acrylic latex paint was also compared.

  3. News at Nine: The value of near-real time data for reaching mass media

    NASA Astrophysics Data System (ADS)

    Allen, J.; Ward, K.; Simmon, R. B.; Carlowicz, M. J.; Scott, M.; Przyborski, P. D.; Voiland, A. P.

    2012-12-01

    NASA's Earth Observatory (EO) is an online publication featuring NASA Earth science news and images. Since its inception in 1999, the EO team has relied heavily on near-real time satellite data to publish imagery of breaking news events, such as volcanoes, floods, fires, and dust storms. Major news outlets (Associated Press, The Weather Channel, CNN, etc.) have regularly republished Earth Observatory imagery in their coverage of events. Because of the nature of modern 24-hour news cycle, media almost always want near-real time coverage; providing it depends heavily on rapid data turnaround, user-friendly data systems, and fast data access. We will discuss how we use near-real time data and provide examples of how data systems have been transformed in the past 13 years. We will offer some thoughts on best practices (from the view of a user) in expedited data systems and the positive effect of those practices on public awareness of our content.. Finally, we will share how we work with science teams to see the potential stories in their data and the value of providing the data in a timely fashionAcquired October 9, 2010, this natural-color image shows the toxic sludge spill from an alumina plant in southern Hungary.

  4. Combining Heat and Mass Flux Methods for Estimating Real-Time Evaporation from a Water Surface

    NASA Astrophysics Data System (ADS)

    Mathis, T. J.; Schladow, G.; Hook, S. J.

    2015-12-01

    Quantifying the heat and mass fluxes associated with evaporation from lakes and reservoirs is achallenge for hydrologists and water managers. This is in large part due to a lack of comprehensivemeasurement data for most systems, which is itself related to the inherent difficulties associated withmeasuring turbulent quantities. An alternative to direct measurement is to develop better models for theevaporative flux, based on the mean terms (as opposed to the turbulent terms) that drive evaporation.Algorithms for the evaporative heat and mass flux must reflect changes in heat storage in the system aswell as the other components of a mass balance (inflow, outflow, and precipitation). The energy budget basedapproach requires records of all the other energy fluxes across the air-water interface to separateout the latent heat component. Other approaches utilize the similarity between atmospheric velocity,temperature and humidity profiles. This study seeks to combine these approaches to build and calibrateheat flux models that can be used to accurately recreate a long-term record of mass storage changefrom a sub-set of meteorological data, lake surface temperature data, and hydrologic observations. Highfrequency lake level data are used to check that the mass balance is in fact achieved. Good agreement isshown between the heat flux methods and the mass balance results through comparison with a three-yearrecord of lake level. The results demonstrate that a combination of mass and heat flux approaches canbe used to generate accurate values of evaporation on daily or even sub-daily time-scales.

  5. Direct Analysis in Real Time (DART) of an Organothiophosphate at Ultrahigh Resolution by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Tandem Mass Spectrometry

    PubMed Central

    Prokai, Laszlo; Stevens, Stanley M.

    2016-01-01

    Direct analysis in real time (DART) is a recently developed ambient ionization technique for mass spectrometry to enable rapid and sensitive analyses with little or no sample preparation. After swab-based field sampling, the organothiophosphate malathion was analyzed using DART-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Mass resolution was documented to be over 800,000 in full-scan MS mode and over 1,000,000 for an MS/MS product ion produced by collision-induced dissociation of the protonated analyte. Mass measurement accuracy below 1 ppm was obtained for all DART-generated ions that belonged to the test compound in the mass spectra acquired using only external mass calibration. This high mass measurement accuracy, achievable at present only through FTMS, was required for unequivocal identification of the corresponding molecular formulae. PMID:26784186

  6. Direct Analysis in Real Time (DART) of an Organothiophosphate at Ultrahigh Resolution by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Tandem Mass Spectrometry.

    PubMed

    Prokai, Laszlo; Stevens, Stanley M

    2016-01-16

    Direct analysis in real time (DART) is a recently developed ambient ionization technique for mass spectrometry to enable rapid and sensitive analyses with little or no sample preparation. After swab-based field sampling, the organothiophosphate malathion was analyzed using DART-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Mass resolution was documented to be over 800,000 in full-scan MS mode and over 1,000,000 for an MS/MS product ion produced by collision-induced dissociation of the protonated analyte. Mass measurement accuracy below 1 ppm was obtained for all DART-generated ions that belonged to the test compound in the mass spectra acquired using only external mass calibration. This high mass measurement accuracy, achievable at present only through FTMS, was required for unequivocal identification of the corresponding molecular formulae.

  7. Determination of the presence or absence of sulfur materials in drywall using direct analysis in real time in conjunction with an accurate-mass time-of-flight mass spectrometer.

    PubMed

    Curtis, Matthew E; Jones, Patrick R; Sparkman, O David; Cody, Robert B

    2009-11-01

    Based on the concern about the presence of sulfur materials being in drywall (wallboard), a quick and reliable test to confirm the presence or absence of these materials using direct analysis in real time (DART) mass spectrometry in conjunction with an accurate-mass time-of-flight (TOF) mass spectrometer has been developed and is described here.

  8. Direct analysis in real time mass spectrometry, a process analytical technology tool for real-time process monitoring in botanical drug manufacturing.

    PubMed

    Wang, Lu; Zeng, Shanshan; Chen, Teng; Qu, Haibin

    2014-03-01

    A promising process analytical technology (PAT) tool has been introduced for batch processes monitoring. Direct analysis in real time mass spectrometry (DART-MS), a means of rapid fingerprint analysis, was applied to a percolation process with multi-constituent substances for an anti-cancer botanical preparation. Fifteen batches were carried out, including ten normal operations and five abnormal batches with artificial variations. The obtained multivariate data were analyzed by a multi-way partial least squares (MPLS) model. Control trajectories were derived from eight normal batches, and the qualification was tested by R(2) and Q(2). Accuracy and diagnosis capability of the batch model were then validated by the remaining batches. Assisted with high performance liquid chromatography (HPLC) determination, process faults were explained by corresponding variable contributions. Furthermore, a batch level model was developed to compare and assess the model performance. The present study has demonstrated that DART-MS is very promising in process monitoring in botanical manufacturing. Compared with general PAT tools, DART-MS offers a particular account on effective compositions and can be potentially used to improve batch quality and process consistency of samples in complex matrices.

  9. REAL TIME, ON-LINE CHARACTERIZATION OF DIESEL GENERATOR AIR TOXIC EMISSIONS BY RESONANCE ENHANCED MULTI-PHOTON IONIZATION TIME OF FLIGHT MASS SPECTROMETRY

    EPA Science Inventory

    The laser based resonance, enhanced multi-photon ionization time-of-flight mass spectrometry (REMPI-TOFMS) technique has been applied to the exhaust gas stream of a diesel generator to measure, in real time, concentration levels of aromatic air toxics. Volatile organic compounds ...

  10. Real-Time Mass Spectrometry Monitoring of Oak Wood Toasting: Elucidating Aroma Development Relevant to Oak-aged Wine Quality

    NASA Astrophysics Data System (ADS)

    Farrell, Ross R.; Wellinger, Marco; Gloess, Alexia N.; Nichols, David S.; Breadmore, Michael C.; Shellie, Robert A.; Yeretzian, Chahan

    2015-11-01

    We introduce a real-time method to monitor the evolution of oak aromas during the oak toasting process. French and American oak wood boards were toasted in an oven at three different temperatures, while the process-gas was continuously transferred to the inlet of a proton-transfer-reaction time-of-flight mass spectrometer for online monitoring. Oak wood aroma compounds important for their sensory contribution to oak-aged wine were tentatively identified based on soft ionization and molecular mass. The time-intensity profiles revealed toasting process dynamics illustrating in real-time how different compounds evolve from the oak wood during toasting. Sufficient sensitivity was achieved to observe spikes in volatile concentrations related to cracking phenomena on the oak wood surface. The polysaccharide-derived compounds exhibited similar profiles; whilst for lignin-derived compounds eugenol formation differed from that of vanillin and guaiacol at lower toasting temperatures. Significant generation of oak lactone from precursors was evident at 225 oC. Statistical processing of the real-time aroma data showed similarities and differences between individual oak boards and oak wood sourced from the different origins. This study enriches our understanding of the oak toasting process and demonstrates a new analytical approach for research on wood volatiles.

  11. Real-Time Mass Spectrometry Monitoring of Oak Wood Toasting: Elucidating Aroma Development Relevant to Oak-aged Wine Quality

    PubMed Central

    Farrell, Ross R.; Wellinger, Marco; Gloess, Alexia N.; Nichols, David S.; Breadmore, Michael C.; Shellie, Robert A.; Yeretzian, Chahan

    2015-01-01

    We introduce a real-time method to monitor the evolution of oak aromas during the oak toasting process. French and American oak wood boards were toasted in an oven at three different temperatures, while the process-gas was continuously transferred to the inlet of a proton-transfer-reaction time-of-flight mass spectrometer for online monitoring. Oak wood aroma compounds important for their sensory contribution to oak-aged wine were tentatively identified based on soft ionization and molecular mass. The time-intensity profiles revealed toasting process dynamics illustrating in real-time how different compounds evolve from the oak wood during toasting. Sufficient sensitivity was achieved to observe spikes in volatile concentrations related to cracking phenomena on the oak wood surface. The polysaccharide-derived compounds exhibited similar profiles; whilst for lignin-derived compounds eugenol formation differed from that of vanillin and guaiacol at lower toasting temperatures. Significant generation of oak lactone from precursors was evident at 225 oC. Statistical processing of the real-time aroma data showed similarities and differences between individual oak boards and oak wood sourced from the different origins. This study enriches our understanding of the oak toasting process and demonstrates a new analytical approach for research on wood volatiles. PMID:26610612

  12. Real-Time Mass Spectrometry Monitoring of Oak Wood Toasting: Elucidating Aroma Development Relevant to Oak-aged Wine Quality.

    PubMed

    Farrell, Ross R; Wellinger, Marco; Gloess, Alexia N; Nichols, David S; Breadmore, Michael C; Shellie, Robert A; Yeretzian, Chahan

    2015-11-27

    We introduce a real-time method to monitor the evolution of oak aromas during the oak toasting process. French and American oak wood boards were toasted in an oven at three different temperatures, while the process-gas was continuously transferred to the inlet of a proton-transfer-reaction time-of-flight mass spectrometer for online monitoring. Oak wood aroma compounds important for their sensory contribution to oak-aged wine were tentatively identified based on soft ionization and molecular mass. The time-intensity profiles revealed toasting process dynamics illustrating in real-time how different compounds evolve from the oak wood during toasting. Sufficient sensitivity was achieved to observe spikes in volatile concentrations related to cracking phenomena on the oak wood surface. The polysaccharide-derived compounds exhibited similar profiles; whilst for lignin-derived compounds eugenol formation differed from that of vanillin and guaiacol at lower toasting temperatures. Significant generation of oak lactone from precursors was evident at 225 (o)C. Statistical processing of the real-time aroma data showed similarities and differences between individual oak boards and oak wood sourced from the different origins. This study enriches our understanding of the oak toasting process and demonstrates a new analytical approach for research on wood volatiles.

  13. Real time mass flux measurements of gas-solid suspensions at low velocities

    SciTech Connect

    Saunders, J H; Chao, B T; Soo, S L

    1981-01-01

    In previous work, measurement of the particulate mass flux was made based upon a novel electrostatic technique. A small conducting wire sensor was inserted in the flow and as each particle hit the sensor an individual pulse of current was identified. Through suitable electronic circuitry, the number of pulses in a given time were counted. This was a direct measure of the number of particle-probe collisions which was related to local particle mass flow. The technique is currently limited to monodisperse suspensions. A primary advantage of the impact counter system is that the output does not depend upon the magnitude of the actual charge transfer. As long as the pulses are sufficiently above the noise level, variations in charge transfer will not affect the measurement. For the current work, the technique was applied to vertical gas-solid flow where the fluid velocity was slightly above the particle terminal velocity. Under these conditions a sufficient signal to noise ratio was not found. The Cheng-Soo charge transfer theory indicated that the low particle-sensor impact velocity was responsible. The probe system was then modified by extracting a particulate sample isokinetically and accelerating the particles to a sufficient velocity by an area reduction in the sampling tube. With this technique the signal to noise ratio was about 12 to 1. Mass flux results are shown to compare favorably with filter collection and weighing.

  14. Detection of nicotine as an indicator of tobacco smoke by direct analysis in real time (DART) tandem mass spectrometry

    NASA Astrophysics Data System (ADS)

    Kuki, Ákos; Nagy, Lajos; Nagy, Tibor; Zsuga, Miklós; Kéki, Sándor

    2015-01-01

    The residual tobacco smoke contamination (thirdhand smoke, THS) on the clothes of a smoker was examined by direct analysis in real time (DART) mass spectrometry. DART-MS enabled sensitive and selective analysis of nicotine as the indicator of tobacco smoke pollution. Tandem mass spectrometric (MS/MS) experiments were also performed to confirm the identification of nicotine. Transferred thirdhand smoke originated from the fingers of a smoker onto other objects was also detected by DART mass spectrometry. DART-MS/MS was utilized for monitoring the secondhand tobacco smoke (SHS) in the air of the laboratory using nicotine as an indicator. To the best of our knowledge, this is the first report on the application of DART-MS and DART-MS/MS to the detection of thirdhand smoke and to the monitoring of secondhand smoke.

  15. Sensitivity "Hot Spots" in the Direct Analysis in Real Time Mass Spectrometry of Nerve Agent Simulants

    NASA Astrophysics Data System (ADS)

    Harris, Glenn A.; Falcone, Caitlin E.; Fernández, Facundo M.

    2012-01-01

    Presented here are findings describing the spatial-dependence of sensitivity and ion suppression effects observed with direct analysis in real time (DART). Continuous liquid infusion of dimethyl methyl phosphonate (DMMP) revealed that ion yield "hot spots" did not always correspond with the highest temperature regions within the ionization space. For instance, at lower concentrations (50 and 100 μM), the highest sensitivities were in the middle of the ionization region at 200 °C where there was a shorter ion transport distance, and the heat available to thermally desorb neutrals was moderate. Conversely, at higher DMMP concentrations (500 μM), the highest ion yield was directly in front of the DART source at 200 °C where it was exposed to the highest temperature for thermal desorption. In matching experiments, differential analyte volatility was observed to play a smaller role in relative ion suppression than differences in proton affinity and the relative sampling positions of analytes. At equimolar concentrations sampled at the same position, suppression was as high as 26× between isoquinoline (proton affinity 952 kJ mol-1, boiling point 242 °C) and p-anisidine (proton affinity 900 kJ mol-1, boiling point 243 °C). This effect was exacerbated when sampling positions of the two analytes differed, reaching levels of relative suppression as high as 4543.0× ± 1406.0. To mitigate this level of relative ion suppression, sampling positions and molar ratios of the analytes were modified to create conditions in which ion suppression was negligible.

  16. Acoustic Emission and Velocity Measurements using a Modular Borehole Prototype Tool to Provide Real Time Rock Mass Characterization.

    NASA Astrophysics Data System (ADS)

    Collins, D. S.; Pettitt, W. S.; Young, R. P.

    2003-04-01

    Permanent changes to rock mass properties can occur due to the application of excavation or thermal induced stresses. This project involves the design of hardware and software for the long term monitoring of a rock volume, and the real time analysis and interpretation of induced microcracks and their properties. A set of borehole sondes have been designed with each sonde containing up to 6 sensor modules. Each piezoelectric sensor is dual mode allowing it to either transmit an ultrasonic pulse through a rock mass, or receive ultrasonic waveform data. Good coupling of the sensors with the borehole wall is achieved through a motorized clamping mechanism. The borehole sondes are connected to a surface interface box and digital acquisition system and controlled by a laptop computer. The system allows acoustic emission (AE) data to be recorded at all times using programmable trigger logic. The AE data is processed in real time for 3D source location and magnitude, with further analysis such as mechanism type available offline. Additionally the system allows velocity surveys to be automatically performed at pre-defined times. A modelling component of the project, using a 3D dynamic finite difference code, is investigating the effect that different microcrack distributions have on velocity waveform data in terms of time and frequency amplitude. The modelling codes will be validated using data recorded from laboratory tests on rocks with known crack fabrics, and then used in insitu experimental tests. This modelling information will be used to help interpret, in real time, microcrack characteristics such as crack density, size, and fluid content. The technology has applications in a number of branches of geotechnical and civil engineering including radioactive waste storage, mining, dams, bridges, and oil reservoir monitoring.

  17. Validation of Real-time Modeling of Coronal Mass Ejections Using the WSA-ENLIL+Cone Heliospheric Model

    NASA Astrophysics Data System (ADS)

    Romano, M.; Mays, M. L.; Taktakishvili, A.; MacNeice, P. J.; Zheng, Y.; Pulkkinen, A. A.; Kuznetsova, M. M.; Odstrcil, D.

    2013-12-01

    Modeling coronal mass ejections (CMEs) is of great interest to the space weather research and forecasting communities. We present recent validation work of real-time CME arrival time predictions at different satellites using the WSA-ENLIL+Cone three-dimensional MHD heliospheric model available at the Community Coordinated Modeling Center (CCMC) and performed by the Space Weather Research Center (SWRC). SWRC is an in-house research-based operations team at the CCMC which provides interplanetary space weather forecasting for NASA's robotic missions and performs real-time model validation. The quality of model operation is evaluated by comparing its output to a measurable parameter of interest such as the CME arrival time and geomagnetic storm strength. The Kp index is calculated from the relation given in Newell et al. (2007), using solar wind parameters predicted by the WSA-ENLIL+Cone model at Earth. The CME arrival time error is defined as the difference between the predicted arrival time and the observed in-situ CME shock arrival time at the ACE, STEREO A, or STEREO B spacecraft. This study includes all real-time WSA-ENLIL+Cone model simulations performed between June 2011-2013 (over 400 runs) at the CCMC/SWRC. We report hit, miss, false alarm, and correct rejection statistics for all three spacecraft. For hits we show the average absolute CME arrival time error, and the dependence of this error on CME input parameters such as speed, width, and direction. We also present the predicted geomagnetic storm strength (using the Kp index) error for Earth-directed CMEs.

  18. Real-Time Quantitative Analysis of H2, He, O2, and Ar by Quadrupole Ion Trap Mass Spectrometry

    NASA Technical Reports Server (NTRS)

    Ottens, Andrew K.; Harrison, W. W.; Griffin, Timothy P.; Helms, William R.; Voska, N. (Technical Monitor)

    2002-01-01

    The use of a quadrupole ion trap mass spectrometer for quantitative analysis of hydrogen and helium as well as other permanent gases is demonstrated. The customized instrument utilizes the mass selective instability mode of mass analysis as with commercial instruments; however, this instrument operates at a greater RF trapping frequency and without a buffer gas. With these differences, a useable mass range from 2 to over 50 Da is achieved, as required by NASA for monitoring the Space Shuttle during a launch countdown. The performance of the ion trap is evaluated using part-per-million concentrations of hydrogen, helium, oxygen and argon mixed into a nitrogen gas stream. Relative accuracy and precision when quantitating the four analytes were better than the NASA-required minimum of 10% error and 5% deviation, respectively. Limits of detection were below the NASA requirement of 25-ppm hydrogen and 100-ppm helium; those for oxygen and argon were slightly higher than the requirement. The instrument provided adequate performance at fast data recording rates, demonstrating the utility of an ion trap mass spectrometer as a real-time quantitative monitoring device for permanent gas analysis.

  19. Polydimethylsiloxane extraction from silicone rubber into baked goods detected by direct analysis in real time mass spectrometry.

    PubMed

    Gross, Jürgen H

    2015-01-01

    Flexible baking molds and other household utensils are made of polydimethylsiloxane (PDMS), also known as silicone rubber. PDMS is prone to release oligomers upon elongated contact with fats, e.g., in the process of baking dough. Positive-ion direct analysis in real time (DART) mass spectrometry (MS) provides an efficient tool for the analysis of PDMS up to m/z 3000. Here, DART ionization is employed in combination with Fourier transform ion cyclotron resonance MS to detect PDMS released into muffins when baked in silicone rubber baking molds. Intensive signals caused by PDMS do occur in the m/z 700-1500 range of DART mass spectra obtained from the crusty surface of muffins after the use of such silicone rubber molds. In addition, triacylglyceroles (TAGs) present as natural ingredients of the analyzed muffins were detected as [TAG+NH(4)](+) ions.

  20. Real-time analysis of self-assembled nucleobases by Venturi easy ambient sonic-spray ionization mass spectrometry.

    PubMed

    Na, Na; Shi, Ruixia; Long, Zi; Lu, Xin; Jiang, Fubin; Ouyang, Jin

    2014-10-01

    In this study, the real-time analysis of self-assembled nucleobases was employed by Venturi easy ambient sonic-spray ionization mass spectrometry (V-EASI-MS). With the analysis of three nucleobases including 6-methyluracil (6MU), uracil (U) and thymine (T) as examples, different orders of clusters centered with different metal ions were recorded in both positive and negative modes. Compared with the results obtained by traditional electrospray ionization mass spectrometry (ESI-MS) under the same condition, more clusters with high orders, such as [6MU7+Na](+), [6MU15+2NH4](2+), [6MU10+Na](+), [T7+Na](+), and [T15+2NH4](2+) were detected by V-EASI-MS, which demonstrated the soft ionization ability of V-EASI for studying the non-covalent interaction in a self-assembly process. Furthermore, with the injection of K(+) to the system by a syringe pumping, the real-time monitoring of the formation of nucleobases clusters was achieved by the direct extraction of samples from the system under the Venturi effect. Therefore, the effect of cations on the formation of clusters during self-assembly of nucleobases was demonstrated, which was in accordance with the reports. Free of high voltage, heating or radiation during the ionization, this technique is much soft and suitable for obtaining the real-time information of the self-assembly system, which also makes it quite convenient for extraction samples from the reaction system. This "easy and soft" ionization technique has provided a potential pathway for monitoring and controlling the self-assembly processes.

  1. Evaluation of a commercial mass spectrometer for its potential to measure auto exhaust constituents in real time

    SciTech Connect

    Dearth, M.A.

    1999-06-01

    A newly available commercial instrument, the V and F Instruments (Austria) AirSense 500 mass spectrometer, was evaluated for its ability to measure various hydrocarbons in auto exhaust, in real time. The measurements obtained were compared to off-line high-resolution gas chromatography and other measurement systems during an evaluation period that extended for more than 7 months and involved various Ford vehicle testing facilities. The device was found to provide real-time (1 Hz or faster) concentration data for 10 hydrocarbon exhaust constituents with a precision and accuracy comparable to current off-line gas chromatographic measurement methods. The AirSense 500 was carefully evaluated for its measurement of ethene and other olefins and benzene and other aromatic compounds. The mass spectral measurements agreed, on average within {+-}17% of the reported values for the same vehicle tests as measured by off-line GC/FID, when comparing the slope of the linear regression line resulting from direct comparison of both techniques. Linear correlations between the two techniques averaged an r{sup 2} of 0.85 for measurements mostly in the 0.01--0.2 ppm range. The instrument was also shown to be useful for the measurement of H{sub 2}S in dilute vehicle exhaust, at levels down to 20 ppb. In comparison to current methods of H{sub 2}S measurement, the AirSense 500 was found to be more robust to potentially interfering compounds.

  2. Early, Real-Time Medical Diagnosis of Botulism by Endopeptidase-Mass Spectrometry.

    PubMed

    Rosen, Osnat; Feldberg, Liron; Gura, Sigalit; Brosh-Nissimov, Tal; Guri, Alex; Zimhony, Oren; Shapiro, Eli; Beth-Din, Adi; Stein, Dana; Ozeri, Eyal; Barnea, Ada; Turgeman, Amram; Ben David, Alon; Schwartz, Arieh; Elhanany, Eytan; Diamant, Eran; Yitzhaki, Shmuel; Zichel, Ran

    2015-12-15

    Botulinum toxin was detected in patient serum using Endopeptidase-mass-spectrometry assay, although all conventional tests provided negative results. Antitoxin was administered, resulting in patient improvement. Implementing this highly sensitive and rapid assay will improve preparedness for foodborne botulism and deliberate exposure.

  3. Real-time monitoring of trace-level VOCs by an ultrasensitive compact lamp-based VUV photoionization mass spectrometer

    NASA Astrophysics Data System (ADS)

    Sun, W. Q.; Shu, J. N.; Zhang, P.; Li, Z.; Li, N. N.; Liang, M.; Yang, B.

    2015-06-01

    In this study, we report on the development of a compact lamp-based vacuum ultraviolet (VUV) photoionization mass spectrometer (PIMS; hereafter referred to as VUV-PIMS) in our laboratory; it is composed of a radio frequency-powered VUV lamp, a VUV photoionizer, an ion-immigration region, and a reflection time-of-flight mass spectrometer. By utilizing the novel photoionizer consisting of a photoionization cavity and a VUV light baffle, extremely low background noise was obtained. An ultrasensitive detection limit (2σ) of 3 pptv was achieved for benzene after an acquisition time of 10 s. To examine its potential for application in real-time sample monitoring, the developed VUV-PIMS was employed for the continuous measurement of urban air for six days in Beijing, China. Strong signals of trace-level volatile organic compounds such as benzene and its alkylated derivatives were observed in the mass spectra. These initial experimental results reveal that the instrument can be used for the online monitoring of trace-level species in the atmosphere.

  4. Real Time Network Assessment

    DTIC Science & Technology

    2013-07-12

    Demonstrate a simple system Conduct a feasibility assessment of data storage, maintenance, and integration requirements Test a web-based data feed...Real Time Network Assessment Prototype We demonstrated the feasibility of linking near real time network analytics to mashups and web- based...combining similar concepts into single node) Stemmers Thesauri application Network position Statistical common patterns Pronoun identification

  5. Real-time monitoring of volatile organic compounds using chemical ionization mass spectrometry

    DOEpatents

    Mowry, Curtis Dale; Thornberg, Steven Michael

    1999-01-01

    A system for on-line quantitative monitoring of volatile organic compounds (VOCs) includes pressure reduction means for carrying a gaseous sample from a first location to a measuring input location maintained at a low pressure, the system utilizing active feedback to keep both the vapor flow and pressure to a chemical ionization mode mass spectrometer constant. A multiple input manifold for VOC and gas distribution permits a combination of calibration gases or samples to be applied to the spectrometer.

  6. Determination of T-2 and HT-2 toxins from maize by direct analysis in real time - mass spectrometry (DART-MS)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ambient desorption ionization techniques, such as laser desorption with electrospray ionization assistance (ELDI), direct analysis in real time (DART) and desorption electrospray ionization (DESI) have been developed as alternatives to traditional mass spectrometric-based methods. Such techniques al...

  7. Direct Analysis in Real Time-Mass Spectrometry for the Rapid Detection of Metabolites of Aconite Alkaloids in Intestinal Bacteria

    NASA Astrophysics Data System (ADS)

    Li, Xue; Hou, Guangyue; Xing, Junpeng; Song, Fengrui; Liu, Zhiqiang; Liu, Shuying

    2014-12-01

    In the present work, direct analysis of real time ionization combined with multi-stage tandem mass spectrometry (DART-MSn) was used to investigate the metabolic profile of aconite alkaloids in rat intestinal bacteria. A total of 36 metabolites from three aconite alkaloids were identified by using DART-MSn, and the feasibility of quantitative analysis of these analytes was examined. Key parameters of the DART ion source, such as helium gas temperature and pressure, the source-to-MS distance, and the speed of the autosampler, were optimized to achieve high sensitivity, enhance reproducibility, and reduce the occurrence of fragmentation. The instrument analysis time for one sample can be less than 10 s for this method. Compared with ESI-MS and UPLC-MS, the DART-MS is more efficient for directly detecting metabolic samples, and has the advantage of being a simple, high-speed, high-throughput method.

  8. Rapid process development of chromatographic process using direct analysis in real time mass spectrometry as a process analytical technology tool.

    PubMed

    Yan, Binjun; Chen, Teng; Xu, Zhilin; Qu, Haibin

    2014-06-01

    The concept of quality by design (QbD) is widely applied in the process development of pharmaceuticals. However, the additional cost and time have caused some resistance about QbD implementation. To show a possible solution, this work proposed a rapid process development method, which used direct analysis in real time mass spectrometry (DART-MS) as a process analytical technology (PAT) tool for studying the chromatographic process of Ginkgo biloba L., as an example. The breakthrough curves were fast determined by DART-MS at-line. A high correlation coefficient of 0.9520 was found between the concentrations of ginkgolide A determined by DART-MS and HPLC. Based on the PAT tool, the impacts of process parameters on the adsorption capacity were discovered rapidly, which showed a decreased adsorption capacity with the increase of the flow rate. This work has shown the feasibility and advantages of integrating PAT into QbD implementation for rapid process development.

  9. Real-time mass spectrometric study of the methanol crossover in a direct methanol fuel cell

    SciTech Connect

    Wang, J.T.; Wasmus, S.; Savinell, R.F.

    1996-04-01

    The products of methanol crossover through the acid-doped polybenzimidazole polymer electrolyte membrane (PBI PEM) to the cathode of a prototype direct methanol fuel cell (DMFC) were analyzed using multipurpose electrochemical mass spectrometry (MPEMS) coupled to the cathode exhaust gas outlet. It was found that the methanol crossing over reacts almost quantitatively to CO{sub 2} at the cathode with the platinum of the cathode acting as a heterogeneous catalyst. The cathode open-circuit potential is inversely proportional to the amount of CO{sub 2} formed. A poisoning effect on the oxygen reduction also was found. Methods for the estimation of the methanol crossover rate at operating fuel cells are suggested.

  10. Rapid determination of melamine and cyanuric acid in milk powder using direct analysis in real time-time-of-flight mass spectrometry.

    PubMed

    Vaclavik, Lukas; Rosmus, Jan; Popping, Bert; Hajslova, Jana

    2010-06-18

    The use of fast semi-automated method employing direct analysis in real time (DART) ion source coupled to time-of-flight mass spectrometry (TOFMS) for analysis of melamine (MEL) and cyanuric acid (CYA) in milk powder and milk based products has been demonstrated in this study. Simple sample extraction procedure employing methanol-5% aqueous formic acid mixture, which enabled disruption of melamine-cyanurate complex, was followed by direct, high-throughput (30s per run) examination of sample extracts spread on a glass rod by mass spectrometry under ambient conditions, without any prior chromatographic separation. After optimization of instrument parameter settings, limits of detection (LODs) 170 and 450microgkg(-1) were achieved for MEL and CYA, respectively. In the final phase of study, the possibility of minimizing spectral interference, thus improving method performance characteristics through the use of ultrahigh resolving power offered by Orbitrap based mass analyzer is demonstrated.

  11. Direct Analysis in Real Time Mass Spectrometry for the Nondestructive Investigation of Conservation Treatments of Cultural Heritage.

    PubMed

    Manfredi, Marcello; Robotti, Elisa; Bearman, Greg; France, Fenella; Barberis, Elettra; Shor, Pnina; Marengo, Emilio

    2016-01-01

    Today the long-term conservation of cultural heritage is a big challenge: often the artworks were subjected to unknown interventions, which eventually were found to be harmful. The noninvasive investigation of the conservation treatments to which they were subjected to is a crucial step in order to undertake the best conservation strategies. We describe here the preliminary results on a quick and direct method for the nondestructive identification of the various interventions of parchment by means of direct analysis in real time (DART) ionization and high-resolution time-of-flight mass spectrometry and chemometrics. The method has been developed for the noninvasive analysis of the Dead Sea Scrolls, one of the most important archaeological discoveries of the 20th century. In this study castor oil and glycerol parchment treatments, prepared on new parchment specimens, were investigated in order to evaluate two different types of operations. The method was able to identify both treatments. In order to investigate the effect of the ion source temperature on the mass spectra, the DART-MS analysis was also carried out at several temperatures. Due to the high sensitivity, simplicity, and no sample preparation requirement, the proposed analytical methodology could help conservators in the challenging analysis of unknown treatments in cultural heritage.

  12. Direct Analysis in Real Time Mass Spectrometry for the Nondestructive Investigation of Conservation Treatments of Cultural Heritage

    PubMed Central

    Bearman, Greg; France, Fenella; Barberis, Elettra; Shor, Pnina; Marengo, Emilio

    2016-01-01

    Today the long-term conservation of cultural heritage is a big challenge: often the artworks were subjected to unknown interventions, which eventually were found to be harmful. The noninvasive investigation of the conservation treatments to which they were subjected to is a crucial step in order to undertake the best conservation strategies. We describe here the preliminary results on a quick and direct method for the nondestructive identification of the various interventions of parchment by means of direct analysis in real time (DART) ionization and high-resolution time-of-flight mass spectrometry and chemometrics. The method has been developed for the noninvasive analysis of the Dead Sea Scrolls, one of the most important archaeological discoveries of the 20th century. In this study castor oil and glycerol parchment treatments, prepared on new parchment specimens, were investigated in order to evaluate two different types of operations. The method was able to identify both treatments. In order to investigate the effect of the ion source temperature on the mass spectra, the DART-MS analysis was also carried out at several temperatures. Due to the high sensitivity, simplicity, and no sample preparation requirement, the proposed analytical methodology could help conservators in the challenging analysis of unknown treatments in cultural heritage. PMID:27957383

  13. Trace analysis of energetic materials via direct analyte-probed nanoextraction coupled to direct analysis in real time mass spectrometry.

    PubMed

    Clemons, Kristina; Dake, Jeffrey; Sisco, Edward; Verbeck, Guido F

    2013-09-10

    Direct analysis in real time mass spectrometry (DART-MS) has proven to be a useful forensic tool for the trace analysis of energetic materials. While other techniques for detecting trace amounts of explosives involve extraction, derivatization, solvent exchange, or sample clean-up, DART-MS requires none of these. Typical DART-MS analyses directly from a solid sample or from a swab have been quite successful; however, these methods may not always be an optimal sampling technique in a forensic setting. For example, if the sample were only located in an area which included a latent fingerprint of interest, direct DART-MS analysis or the use of a swab would almost certainly destroy the print. To avoid ruining such potentially invaluable evidence, another method has been developed which will leave the fingerprint virtually untouched. Direct analyte-probed nanoextraction coupled to nanospray ionization-mass spectrometry (DAPNe-NSI-MS) has demonstrated excellent sensitivity and repeatability in forensic analyses of trace amounts of illicit drugs from various types of surfaces. This technique employs a nanomanipulator in conjunction with bright-field microscopy to extract single particles from a surface of interest and has provided a limit of detection of 300 attograms for caffeine. Combining DAPNe with DART-MS provides another level of flexibility in forensic analysis, and has proven to be a sufficient detection method for trinitrotoluene (TNT), RDX, and 1-methylaminoanthraquinone (MAAQ).

  14. An improved method for the determination of 5-hydroxymethylfurfural in Shenfu injection by direct analysis in real time-quadrupole time-of-flight mass spectrometry.

    PubMed

    Gao, Wen; Qi, Lian-Wen; Liu, Charles C; Wang, Rui; Li, Ping; Yang, Hua

    2016-07-01

    The emergence of direct analysis in real time (DART) ion source provides the great possibility for rapid analysis of hazardous substance in drugs. DART mass spectrometry (DART-MS) enabled the conducting of a fast and non-contact analysis of various samples, including solid or liquid ones, without complex sample preparation or chromatographic separation. In this study, a modified DART-quadrupole time-of-flight mass spectrometry (DART-QTOF-MS) method was developed for identification and determination of 5-hydroxymethylfurfural (5-HMF) in Shenfu (SF) injection. The quantitative transfer of sample solution was introduced to the glass tips of DIP-it sampler at a fixed volume, which significantly increases the repeatability and accuracy of analytical results. The protonated ion of dibutyl phthalate in the atmosphere was used as the reference mass for TOF-MS recalibration during the data acquisition for constant high accuracy mass measurements. Finally, the developed DART-MS method was used to determine 5-HMF in seven batches of SF injection, and the contents of 5-HMF were not higher than 100 µg/mL. The results obtained were further confirmed by an ultra-high performance liquid chromatography combined with triple quadrupole mass spectrometer (UHPLC-QQQ-MS). The overall results demonstrated that the DART-QTOF-MS method could be applied as an alternative technique for rapid monitoring 5-HMF in herbal medicine injection. Copyright © 2015 John Wiley & Sons, Ltd.

  15. Purification of pharmaceutical preparations using thin-layer chromatography to obtain mass spectra with Direct Analysis in Real Time and accurate mass spectrometry.

    PubMed

    Wood, Jessica L; Steiner, Robert R

    2011-06-01

    Forensic analysis of pharmaceutical preparations requires a comparative analysis with a standard of the suspected drug in order to identify the active ingredient. Purchasing analytical standards can be expensive or unattainable from the drug manufacturers. Direct Analysis in Real Time (DART™) is a novel, ambient ionization technique, typically coupled with a JEOL AccuTOF™ (accurate mass) mass spectrometer. While a fast and easy technique to perform, a drawback of using DART™ is the lack of component separation of mixtures prior to ionization. Various in-house pharmaceutical preparations were purified using thin-layer chromatography (TLC) and mass spectra were subsequently obtained using the AccuTOF™- DART™ technique. Utilizing TLC prior to sample introduction provides a simple, low-cost solution to acquiring mass spectra of the purified preparation. Each spectrum was compared against an in-house molecular formula list to confirm the accurate mass elemental compositions. Spectra of purified ingredients of known pharmaceuticals were added to an in-house library for use as comparators for casework samples. Resolving isomers from one another can be accomplished using collision-induced dissociation after ionization. Challenges arose when the pharmaceutical preparation required an optimized TLC solvent to achieve proper separation and purity of the standard. Purified spectra were obtained for 91 preparations and included in an in-house drug standard library. Primary standards would only need to be purchased when pharmaceutical preparations not previously encountered are submitted for comparative analysis. TLC prior to DART™ analysis demonstrates a time efficient and cost saving technique for the forensic drug analysis community. Copyright © 2011 John Wiley & Sons, Ltd.

  16. Real-time radiography

    SciTech Connect

    Bossi, R.H.; Oien, C.T.

    1981-02-26

    Real-time radiography is used for imaging both dynamic events and static objects. Fluorescent screens play an important role in converting radiation to light, which is then observed directly or intensified and detected. The radiographic parameters for real-time radiography are similar to conventional film radiography with special emphasis on statistics and magnification. Direct-viewing fluoroscopy uses the human eye as a detector of fluorescent screen light or the light from an intensifier. Remote-viewing systems replace the human observer with a television camera. The remote-viewing systems have many advantages over the direct-viewing conditions such as safety, image enhancement, and the capability to produce permanent records. This report reviews real-time imaging system parameters and components.

  17. Real time obscuration monitoring

    NASA Astrophysics Data System (ADS)

    Agricola, Koos

    2016-09-01

    Recently a real time particle deposition monitoring system is developed. After discussions with optical system engineers a new feature has been added. This enables the real time monitoring of obscuration of exposed optical components by counting the deposited particles and sizing the obscuration area of each particle. This way the Particle Obscuration Rate (POR) can be determined. The POR can be used to determine the risk of product contamination during exposure. The particle size distribution gives information on the type of potential particle sources. The deposition moments will indicate when these sources were present.

  18. Analysis of silicones released from household items and baby articles by direct analysis in real time-mass spectrometry.

    PubMed

    Gross, Jürgen H

    2015-03-01

    Direct analysis in real time-mass spectrometry (DART-MS) enables screening of articles of daily use made of polydimethylsiloxanes (PDMS), commonly known as silicone rubber, to assess their tendency to release low molecular weight silicone oligomers. DART-MS analyses were performed on a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. Flexible silicone baking molds, a watch band, and a dough scraper, as baby articles different brands of pacifiers, nipples, and a teething ring have been examined. While somewhat arbitrarily chosen, the set can be regarded as representative of household items, baby articles, and other objects made of silicone rubber. For comparison, two brands of silicone septa and as blanks a glass slide and a latex pacifier were included. Differences between the objects were mainly observed in terms of molecular weight distribution and occasional release of other compounds in addition to PDMS. Other than that, all objects made of silicone rubber released significant amounts of PDMS during DART analysis. To provide a coarse quantification, a calibration based on silicone oil was established, which delivered PDMS losses from 20 μg to >100 μg during the 16-s period per measurement. Also, the extraction of baking molds in rapeseed oil demonstrated a PDMS release at the level of 1 μg mg(-1). These findings indicate a potential health hazard from frequent or long-term use of such items. This work does not intend to blame certain brands of such articles. Nonetheless, a higher level of awareness of this source of daily silicone intake is suggested.

  19. Analysis of Silicones Released from Household Items and Baby Articles by Direct Analysis in Real Time-Mass Spectrometry

    NASA Astrophysics Data System (ADS)

    Gross, Jürgen H.

    2015-03-01

    Direct analysis in real time-mass spectrometry (DART-MS) enables screening of articles of daily use made of polydimethylsiloxanes (PDMS), commonly known as silicone rubber, to assess their tendency to release low molecular weight silicone oligomers. DART-MS analyses were performed on a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. Flexible silicone baking molds, a watch band, and a dough scraper, as baby articles different brands of pacifiers, nipples, and a teething ring have been examined. While somewhat arbitrarily chosen, the set can be regarded as representative of household items, baby articles, and other objects made of silicone rubber. For comparison, two brands of silicone septa and as blanks a glass slide and a latex pacifier were included. Differences between the objects were mainly observed in terms of molecular weight distribution and occasional release of other compounds in addition to PDMS. Other than that, all objects made of silicone rubber released significant amounts of PDMS during DART analysis. To provide a coarse quantification, a calibration based on silicone oil was established, which delivered PDMS losses from 20 μg to >100 μg during the 16-s period per measurement. Also, the extraction of baking molds in rapeseed oil demonstrated a PDMS release at the level of 1 μg mg-1. These findings indicate a potential health hazard from frequent or long-term use of such items. This work does not intend to blame certain brands of such articles. Nonetheless, a higher level of awareness of this source of daily silicone intake is suggested.

  20. Plant seed species identification from chemical fingerprints: a high-throughput application of direct analysis in real time mass spectrometry.

    PubMed

    Lesiak, Ashton D; Cody, Robert B; Dane, A John; Musah, Rabi A

    2015-09-01

    Plant species identification based on the morphological features of plant parts is a well-established science in botany. However, species identification from seeds has largely been unexplored, despite the fact that the seeds contain all of the genetic information that distinguishes one plant from another. Using seeds of genus Datura plants, we show here that the mass spectrum-derived chemical fingerprints for seeds of the same species are similar. On the other hand, seeds from different species within the same genus display distinct chemical signatures, even though they may contain similar characteristic biomarkers. The intraspecies chemical signature similarities on the one hand, and interspecies fingerprint differences on the other, can be processed by multivariate statistical analysis methods to enable rapid species-level identification and differentiation. The chemical fingerprints can be acquired rapidly and in a high-throughput manner by direct analysis in real time mass spectrometry (DART-MS) analysis of the seeds in their native form, without use of a solvent extract. Importantly, knowledge of the identity of the detected molecules is not required for species level identification. However, confirmation of the presence within the seeds of various characteristic tropane and other alkaloids, including atropine, scopolamine, scopoline, tropine, tropinone, and tyramine, was accomplished by comparison of the in-source collision-induced dissociation (CID) fragmentation patterns of authentic standards, to the fragmentation patterns observed in the seeds when analyzed under similar in-source CID conditions. The advantages, applications, and implications of the chemometric processing of DART-MS derived seed chemical signatures for species level identification and differentiation are discussed.

  1. Determination of the aflatoxin AFB1 from corn by direct analysis in real time-mass spectrometry (DART-MS).

    PubMed

    Busman, Mark; Liu, Jihong; Zhong, Hongjian; Bobell, John R; Maragos, Chris M

    2014-01-01

    Direct analysis in real time (DART) ionisation coupled to a high-resolution mass spectrometer (MS) was used for screening of aflatoxins from a variety of surfaces and the rapid quantitative analysis of a common form of aflatoxin, AFB1, extracted from corn. Sample preparation procedure and instrument parameter settings were optimised to obtain sensitive and accurate determination of aflatoxin AFB1. 84:16 acetonitrile water extracts of corn were analysed by DART-MS. The lowest calibration level (LCL) for aflatoxin AFB1 was 4 μg kg⁻¹. Quantitative analysis was performed with the use of matrix-matched standards employing the ¹³C-labelled internal standard for AFB1. DART-MS of spiked corn extracts gave linear response in the range 4-1000 μg kg⁻¹. Good recoveries (94-110%) and repeatabilities (RSD = 0.7-6.9%) were obtained at spiking levels of 20 and 100 μg kg⁻¹ with the use of an isotope dilution technique. Trueness of data obtained for AFB1 in maize by DART-MS was demonstrated by analysis of corn certified reference materials.

  2. Monolith dip-it: a bifunctional device for improving the sensitivity of direct analysis in real time mass spectrometry.

    PubMed

    Li, Xianjiang; Li, Ze; Wang, Xin; Nie, Honggang; Zhang, Yiding; Bai, Yu; Liu, Huwei

    2016-08-02

    A bifunctional monolith dip-it was fabricated and applied for improving the sensitivity of direct analysis in real time mass spectrometry (DART-MS). This monolith dip-it device was prepared by in situ polymerization of poly(BMA-EDMA-MAA) monolith in the glass capillary of dip-it. As a solid-phase microextraction (SPME) device, it showed strong affinity to four Sudan dyes through hydrophilic interaction and hydrogen bond interaction. As a sample loading device, it could be directly analyzed by DART-MS without organic solvent elution or laser desorption. As a result, this device is environmentally friendly, and used for fast analysis. Under optimized conditions, the limits of detection for four analytes were 5-10 ng mL(-1) and the linear ranges covered more than two orders of magnitude. Finally, the developed method has been applied for the analysis of chili powder and the recoveries for spiked analytes were in the range of 83.2% to 115.1% demonstrating that this device is an efficient sampler for DART-MS analysis and the proposed method could find more applications in different areas like food analysis.

  3. Rapid determination of 1-deoxynojirimycin in Morus alba L. leaves by direct analysis in real time (DART) mass spectrometry.

    PubMed

    Xu, Bin; Zhang, Dong-Yang; Liu, Ze-Yu; Zhang, Ying; Liu, Li; Li, Long; Liu, Charles C; Wu, Guo-Hua

    2015-10-10

    A new method based on a Direct Analysis in Real Time (DART) ionization source coupled with triple quadrupole tandem mass spectrometry has been developed for rapid qualitative and quantitative analyses of 1-deoxynojirimycin (DNJ) in mulberry leaves. Two ions produced from DNJ, [M+H](+) (m/z 164) and [M-2H+H](+) (m/z 162), are observed using DART-MS in the positive ion mode. The peak areas of the two selected ions monitoring (SIM) signals of ([M+H](+) (m/z 164) and [M-2H+H](+) (m/z 162)) are integrated to determine the peak area for quantitative analyses. A reasonable linear regression equation is obtained in the range of 1.01 to 40.50 μg/mL, with a linear coefficient (R(2)) of 0.996. The limits of detection (LOD) and quantification (LOQ) of the method are 0.25 and 0.80 μg/mL, respectively. The range of recovery is shown to be 87.73-95.61%. The results derived from the developed DART-MS method are in good agreement with those from the conventional HPLC-FLD method. By contrast, DART-MS in SIM mode is a simple, rapid and high-throughput approach for the determination of the DNJ content in mulberry leaves. The present method is advantageous for the rapid screening of mulberry leaves containing high DNJ contents.

  4. Real-time mass spectroscopy analysis of Li-ion battery electrolyte degradation under abusive thermal conditions

    NASA Astrophysics Data System (ADS)

    Gaulupeau, B.; Delobel, B.; Cahen, S.; Fontana, S.; Hérold, C.

    2017-02-01

    The lithium-ion batteries are widely used in rechargeable electronic devices. The current challenges are to improve the capacity and safety of these systems in view of their development to a larger scale, such as for their application in electric and hybrid vehicles. Lithium-ion batteries use organic solvents because of the wide operating voltage. The corresponding electrolytes are usually based on combinations of linear, cyclic alkyl carbonates and a lithium salt such as LiPF6. It has been reported that in abusive thermal conditions, a catalytic effect of the cathode materials lead to the formation fluoro-organics compounds. In order to understand the degradation phenomenon, the study at 240 °C of the interaction between positive electrode materials (LiCoO2, LiNi1/3Mn1/3Co1/3O2, LiMn2O4 and LiFePO4) and electrolyte in dry and wet conditions has been realized by an original method which consists in analyzing by mass spectrometry in real time the volatile molecules produced. The evolution of specific gases channels coupled to the NMR reveal the formation of rarely discussed species such as 2-fluoroethanol and 1,4-dioxane. Furthermore, it appears that the presence of water or other protic impurities greatly influence their formation.

  5. Argon direct analysis in real time mass spectrometry in conjunction with makeup solvents: a method for analysis of labile compounds.

    PubMed

    Yang, Hongmei; Wan, Debin; Song, Fengrui; Liu, Zhiqiang; Liu, Shuying

    2013-02-05

    Helium direct analysis in real time (He-DART) mass spectrometry (MS) analysis of labile compounds usually tends to be challenging because of the occurrence of prominent fragmentation, which obscures the assigning of an ion to an independent species or merely a fragment in a mixture. In the present work, argon DART (Ar-DART) MS in conjunction with makeup solvents has been demonstrated to analyze a variety of labile compounds including nucleosides, alkaloids, glucose, and other small molecules. The results presented here confirm that Ar-DART can generate significantly less energetic ions than conventional He-DART and is able to produce the intact molecular ions with little or no fragmentation in both positive and negative ion modes. Adding a makeup solvent (absolute ethyl alcohol, methanol, fluorobenzene, or acetone) to the argon gas stream at the exit of the DART ion source can result in 1-2 orders of magnitude increase in detection signals. The sensitivity attainable by Ar-DART was found to be comparable to that by He-DART. The investigation of influence of solvents improves our understanding of the fundamental desorption and ionization processes in DART. The practical application of this rapid and high throughput method is demonstrated by the successful analysis of a natural product (Crude Kusnezoff Monkshood) extract, demonstrating the great potential in mixture research.

  6. Improvement in ionization efficiency of direct analysis in real time-mass spectrometry (DART-MS) by corona discharge.

    PubMed

    Sekimoto, Kanako; Sakakura, Motoshi; Kawamukai, Takatomo; Hike, Hiroshi; Shiota, Teruhisa; Usui, Fumihiko; Bando, Yasuhiko; Takayama, Mitsuo

    2016-08-02

    Herein it is shown that a combination of direct analysis in real time (DART) with a corona discharge system consisting of only a needle electrode easily improves DART ionization efficiency. Positive and negative DC corona discharges led to a formation of abundant excited helium atoms as well as the reactant ions H3O(+)(H2O)n and O2˙(-) in the DART analyte ionization area. These phenomena resulted in an increase in the absolute intensities of (de)protonated analytes by a factor of 2-20 over conventional DART. The other analyte ions detected in this corona-DART system (i.e., molecular ions, fragment ions, oxygenated (de)protonated analytes, dehydrogenated deprotonated analytes, and negative ion adducts) were quite similar to those obtained from DART alone. This indicates a lack of side reactions due to the corona discharge. The change in the relative intensities of individual analyte-related ions due to the combination of a corona discharge system with DART suggests that there is no effect of the abundant excited helium in the analyte ionization area on the fragmentation processes or enhancement of oxidation due to hydroxyl radicals HO˙. Furthermore, it was found that the corona-DART combination can be applied to the highly sensitive analysis of n-alkanes, in which the alkanes are ionized as positive ions via hydride abstraction and oxidation, independent of the type of alkane or the mass spectrometer used.

  7. Real-time cosmology

    NASA Astrophysics Data System (ADS)

    Quercellini, Claudia; Amendola, Luca; Balbi, Amedeo; Cabella, Paolo; Quartin, Miguel

    2012-12-01

    In recent years, improved astrometric and spectroscopic techniques have opened the possibility of measuring the temporal change of radial and transverse position of sources in the sky over relatively short time intervals. This has made at least conceivable to establish a novel research domain, which we dub “real-time cosmology”. We review for the first time most of the work already done in this field, analysing the theoretical framework as well as some foreseeable observational strategies and their capability to constrain models. We first focus on real-time measurements of the overall redshift drift and angular separation shift in distant sources, which allows the observer to trace the background cosmic expansion and large scale anisotropy, respectively. We then examine the possibility of employing the same kind of observations to probe peculiar and proper accelerations in clustered systems, and therefore their gravitational potential. The last two sections are devoted to the future change of the cosmic microwave background on “short” time scales, as well as to the temporal shift of the temperature anisotropy power spectrum and maps. We conclude revisiting in this context the usefulness of upcoming experiments (like CODEX and Gaia) for real-time observations.

  8. In cleanroom, sub-ppb real-time monitoring of volatile organic compounds using proton-transfer reaction/time of flight/mass spectrometry

    NASA Astrophysics Data System (ADS)

    Hayeck, Nathalie; Maillot, Philippe; Vitrani, Thomas; Pic, Nicolas; Wortham, Henri; Gligorovski, Sasho; Temime-Roussel, Brice; Mizzi, Aurélie; Poulet, Irène

    2014-04-01

    Refractory compounds such as Trimethylsilanol (TMS) and other organic compounds such as propylene glycol methyl ether acetate (PGMEA) used in the photolithography area of microelectronic cleanrooms have irreversible dramatic impact on optical lenses used on photolithography tools. There is a need for real-time, continuous measurements of organic contaminants in representative cleanroom environment especially in lithography zone. Such information is essential to properly evaluate the impact of organic contamination on optical lenses. In this study, a Proton-Transfer Reaction-Time-of-Flight Mass spectrometer (PTR-TOF-MS) was applied for real-time and continuous monitoring of fugitive organic contamination induced by the fabrication process. Three types of measurements were carried out using the PTR-TOF-MS in order to detect the volatile organic compounds (VOCs) next to the tools in the photolithography area and at the upstream and downstream of chemical filters used to purge the air in the cleanroom environment. A validation and verification of the results obtained with PTR-TOF-MS was performed by comparing these results with those obtained with an off-line technique that is Automated Thermal Desorber - Gas Chromatography - Mass Spectrometry (ATD-GC-MS) used as a reference analytical method. The emerged results from the PTR-TOF-MS analysis exhibited the temporal variation of the VOCs levels in the cleanroom environment during the fabrication process. While comparing the results emerging from the two techniques, a good agreement was found between the results obtained with PTR-TOF-MS and those obtained with ATD-GC-MS for the PGMEA, toluene and xylene. Regarding TMS, a significant difference was observed ascribed to the technical performance of both instruments.

  9. Real time Faraday spectrometer

    DOEpatents

    Smith, Jr., Tommy E.; Struve, Kenneth W.; Colella, Nicholas J.

    1991-01-01

    This invention uses a dipole magnet to bend the path of a charged particle beam. As the deflected particles exit the magnet, they are spatially dispersed in the bend-plane of the magnet according to their respective momenta and pass to a plurality of chambers having Faraday probes positioned therein. Both the current and energy distribution of the particles is then determined by the non-intersecting Faraday probes located along the chambers. The Faraday probes are magnetically isolated from each other by thin metal walls of the chambers, effectively providing real time current-versus-energy particle measurements.

  10. [Real time 3D echocardiography

    NASA Technical Reports Server (NTRS)

    Bauer, F.; Shiota, T.; Thomas, J. D.

    2001-01-01

    Three-dimensional representation of the heart is an old concern. Usually, 3D reconstruction of the cardiac mass is made by successive acquisition of 2D sections, the spatial localisation and orientation of which require complex guiding systems. More recently, the concept of volumetric acquisition has been introduced. A matricial emitter-receiver probe complex with parallel data processing provides instantaneous of a pyramidal 64 degrees x 64 degrees volume. The image is restituted in real time and is composed of 3 planes (planes B and C) which can be displaced in all spatial directions at any time during acquisition. The flexibility of this system of acquisition allows volume and mass measurement with greater accuracy and reproducibility, limiting inter-observer variability. Free navigation of the planes of investigation allows reconstruction for qualitative and quantitative analysis of valvular heart disease and other pathologies. Although real time 3D echocardiography is ready for clinical usage, some improvements are still necessary to improve its conviviality. Then real time 3D echocardiography could be the essential tool for understanding, diagnosis and management of patients.

  11. Rapidly differentiating grape seeds from different sources based on characteristic fingerprints using direct analysis in real time coupled with time-of-flight mass spectrometry combined with chemometrics.

    PubMed

    Song, Yuqiao; Liao, Jie; Dong, Junxing; Chen, Li

    2015-09-01

    The seeds of grapevine (Vitis vinifera) are a byproduct of wine production. To examine the potential value of grape seeds, grape seeds from seven sources were subjected to fingerprinting using direct analysis in real time coupled with time-of-flight mass spectrometry combined with chemometrics. Firstly, we listed all reported components (56 components) from grape seeds and calculated the precise m/z values of the deprotonated ions [M-H](-) . Secondly, the experimental conditions were systematically optimized based on the peak areas of total ion chromatograms of the samples. Thirdly, the seven grape seed samples were examined using the optimized method. Information about 20 grape seed components was utilized to represent characteristic fingerprints. Finally, hierarchical clustering analysis and principal component analysis were performed to analyze the data. Grape seeds from seven different sources were classified into two clusters; hierarchical clustering analysis and principal component analysis yielded similar results. The results of this study lay the foundation for appropriate utilization and exploitation of grape seed samples. Due to the absence of complicated sample preparation methods and chromatographic separation, the method developed in this study represents one of the simplest and least time-consuming methods for grape seed fingerprinting.

  12. Soft Ionization of Saturated Hydrocarbons, Alcohols and Nonpolar Compounds by Negative-Ion Direct Analysis in Real-Time Mass Spectrometry

    NASA Astrophysics Data System (ADS)

    Cody, Robert B.; Dane, A. John

    2013-03-01

    Large polarizable n-alkanes (approximately C18 and larger), alcohols, and other nonpolar compounds can be detected as negative ions when sample solutions are injected directly into the sampling orifice of the atmospheric pressure interface of the time-of-flight mass spectrometer with the direct analysis in real time (DART) ion source operating in negative-ion mode. The mass spectra are dominated by peaks corresponding to [M + O2]‾•. No fragmentation is observed, making this a very soft ionization technique for samples that are otherwise difficult to analyze by DART. Detection limits for cholesterol were determined to be in the low nanogram range.

  13. Real-Time 3D Fluoroscopy-Guided Large Core Needle Biopsy of Renal Masses: A Critical Early Evaluation According to the IDEAL Recommendations

    SciTech Connect

    Kroeze, Stephanie G. C.; Huisman, Merel; Verkooijen, Helena M.; Diest, Paul J. van; Ruud Bosch, J. L. H.; Bosch, Maurice A. A. J. van den

    2012-06-15

    Introduction: Three-dimensional (3D) real-time fluoroscopy cone beam CT is a promising new technique for image-guided biopsy of solid tumors. We evaluated the technical feasibility, diagnostic accuracy, and complications of this technique for guidance of large-core needle biopsy in patients with suspicious renal masses. Methods: Thirteen patients with 13 suspicious renal masses underwent large-core needle biopsy under 3D real-time fluoroscopy cone beam CT guidance. Imaging acquisition and subsequent 3D reconstruction was done by a mobile flat-panel detector (FD) C-arm system to plan the needle path. Large-core needle biopsies were taken by the interventional radiologist. Technical success, accuracy, and safety were evaluated according to the Innovation, Development, Exploration, Assessment, Long-term study (IDEAL) recommendations. Results: Median tumor size was 2.6 (range, 1.0-14.0) cm. In ten (77%) patients, the histological diagnosis corresponded to the imaging findings: five were malignancies, five benign lesions. Technical feasibility was 77% (10/13); in three patients biopsy results were inconclusive. The lesion size of these three patients was <2.5 cm. One patient developed a minor complication. Median follow-up was 16.0 (range, 6.4-19.8) months. Conclusions: 3D real-time fluoroscopy cone beam CT-guided biopsy of renal masses is feasible and safe. However, these first results suggest that diagnostic accuracy may be limited in patients with renal masses <2.5 cm.

  14. Quantitative real-time monitoring of chemical reactions by autosampling flow injection analysis coupled with atmospheric pressure chemical ionization mass spectrometry.

    PubMed

    Zhu, Zhenqian; Bartmess, John E; McNally, Mary Ellen; Hoffman, Ron M; Cook, Kelsey D; Song, Liguo

    2012-09-04

    Although qualitative and/or semiquantitative real-time monitoring of chemical reactions have been reported with a few mass spectrometric approaches, to our knowledge, no quantitative mass spectrometric approach has been reported so far to have a calibration valid up to molar concentrations as required by process control. This is mostly due to the absence of a practical solution that could well address the sample overloading issue. In this study, a novel autosampling flow injection analysis coupled with an atmospheric pressure chemical ionization mass spectrometry (FIA/APCI-MS) system, consisting of a 1 μL automatic internal sample injector, a postinjection splitter with 1:10 splitting ratio, and a detached APCI source connected to the mass spectrometer using a 4.5 in. long, 0.042 in. inner diameter (ID) stainless-steel capillary, was thus introduced. Using this system together with an optional FIA solvent modifier, e.g., 0.05% (v/v) isopropylamine, a linear quantitative calibration up to molar concentration has been achieved with 3.4-7.2% relative standard deviations (RSDs) for 4 replicates. As a result, quantitative real-time monitoring of a model reaction was successfully performed at the 1.63 M level. It is expected that this novel autosampling FIA/APCI-MS system can be used in quantitative real-time monitoring of a wide range of reactions under diverse reaction conditions.

  15. Combined use of direct analysis in real-time/Orbitrap mass spectrometry and micro-Raman spectroscopy for the comprehensive characterization of real explosive samples.

    PubMed

    Bridoux, Maxime C; Schwarzenberg, Adrián; Schramm, Sébastien; Cole, Richard B

    2016-08-01

    Direct Analysis in Real Time (DART™) high-resolution Orbitrap™ mass spectrometry (HRMS) in combination with Raman microscopy was used for the detailed molecular level characterization of explosives including not only the charge but also the complex matrix of binders, plasticizers, polymers, and other possible organic additives. A total of 15 defused military weapons including grenades, mines, rockets, submunitions, and mortars were examined. Swabs and wipes were used to collect trace (residual) amounts of explosives and their organic constituents from the defused military weapons and micrometer-size explosive particles were transferred using a vacuum suction-impact collection device (vacuum impactor) from wipe and swap samples to an impaction plate made of carbon. The particles deposited on the carbon plate were then characterized using micro-Raman spectroscopy followed by DART-HRMS providing fingerprint signatures of orthogonal nature. The optical microscope of the micro-Raman spectrometer was first used to localize and characterize the explosive charge on the impaction plate which was then targeted for identification by DART-HRMS analysis in both the negative and positive modes. Raman spectra of the explosives TNT, RDX and PETN were acquired from micrometer size particles and characterized by the presence of their characteristic Raman bands obtained directly at the surface of the impaction plate nondestructively without further sample preparation. Negative mode DART-HRMS confirmed the types of charges contained in the weapons (mainly TNT, RDX, HMX, and PETN; either as individual components or as mixtures). These energetic compounds were mainly detected as deprotonated species [M-H](-), or as adduct [M + (35)Cl](-), [M + (37)Cl](-), or [M + NO3](-) anions. Chloride adducts were promoted in the heated DART reagent gas by adding chloroform vapors to the helium stream using an "in-house" delivery method. When the polarity was switched to positive mode

  16. Barrow real-time sea ice mass balance data: ingestion, processing, dissemination and archival of multi-sensor data

    NASA Astrophysics Data System (ADS)

    Grimes, J.; Mahoney, A. R.; Heinrichs, T. A.; Eicken, H.

    2012-12-01

    Sensor data can be highly variable in nature and also varied depending on the physical quantity being observed, sensor hardware and sampling parameters. The sea ice mass balance site (MBS) operated in Barrow by the University of Alaska Fairbanks (http://seaice.alaska.edu/gi/observatories/barrow_sealevel) is a multisensor platform consisting of a thermistor string, air and water temperature sensors, acoustic altimeters above and below the ice and a humidity sensor. Each sensor has a unique specification and configuration. The data from multiple sensors are combined to generate sea ice data products. For example, ice thickness is calculated from the positions of the upper and lower ice surfaces, which are determined using data from downward-looking and upward-looking acoustic altimeters above and below the ice, respectively. As a data clearinghouse, the Geographic Information Network of Alaska (GINA) processes real time data from many sources, including the Barrow MBS. Doing so requires a system that is easy to use, yet also offers the flexibility to handle data from multisensor observing platforms. In the case of the Barrow MBS, the metadata system needs to accommodate the addition of new and retirement of old sensors from year to year as well as instrument configuration changes caused by, for example, spring melt or inquisitive polar bears. We also require ease of use for both administrators and end users. Here we present the data and processing steps of using sensor data system powered by the NoSQL storage engine, MongoDB. The system has been developed to ingest, process, disseminate and archive data from the Barrow MBS. Storing sensor data in a generalized format, from many different sources, is a challenging task, especially for traditional SQL databases with a set schema. MongoDB is a NoSQL (not only SQL) database that does not require a fixed schema. There are several advantages using this model over the traditional relational database management system (RDBMS

  17. Real time polarimetric dehazing.

    PubMed

    Mudge, Jason; Virgen, Miguel

    2013-03-20

    Remote sensing is a rich topic due to its utility in gathering detailed accurate information from locations that are not economically feasible traveling destinations or are physically inaccessible. However, poor visibility over long path lengths is problematic for a variety of reasons. Haze induced by light scatter is one cause for poor visibility and is the focus of this article. Image haze comes about as a result of light scattering off particles and into the imaging path causing a haziness to appear on the image. Image processing using polarimetric information of light scatter can be used to mitigate image haze. An imaging polarimeter which provides the Stokes values in real time combined with a "dehazing" algorithm can automate image haze removal for instant applications. Example uses are to improve visual display providing on-the-spot detection or imbedding in an active control loop to improve viewing and tracking while on a moving platform. In addition, removing haze in this manner allows the trade space for a system operational waveband to be opened up to bands which are object matched and not necessarily restricted by scatter effects.

  18. Analysis of carbohydrates in Fusarium verticillioides using size-exclusion HPLC – DRI and direct analysis in real time ionization – time-of-flight – mass spectrometry (DART-MS)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Direct analysis in real time ionization – time-of-flight – mass spectrometry (DART-MS) and size-exclusion HPLC – DRI are used, respectively, to qualitatively and quantitatively determine the carbohydrates extracted from the corn rot fungus Fusarium verticillioides. In situ permethylation in the DART...

  19. Rapid Discrimination of Closely Related Seed Herbs (Cumin, Caraway, and Fennel) by Direct Analysis in Real Time Mass Spectrometry (DART-MS).

    PubMed

    Antal, Borbála; Kuki, Ákos; Nagy, Lajos; Nagy, Tibor; Zsuga, Miklós; M-Hamvas, Márta; Vasas, Gábor; Kéki, Sándor

    2016-01-01

    Direct analysis in real time mass spectrometry (DART-MS) was applied as a rapid method for the discrimination of the spices and traditional medicines cumin (Cuminum cyminum L.), caraway (Carum carvi L.), and fennel (Foeniculum vulgare Mill.). The seeds of these plants were analyzed without sample preparation by DART ion source coupled with quadrupole time-of-flight (QTOF) tandem mass spectrometry. The relatively clean DART spectra showed characteristic patterns, fingerprints, for each herb. It was found that a marker compound can be assigned to each species that can identify unambiguously these plants. Principal component analysis has also been used to analyze the DART-MS data of these seed herbs. Crispanone, carvone, and fenchone are the dominant compounds in the positive DART spectra of cumin, caraway, and fennel, respectively. Crispanone was first time identified as a constituent of cumin. Furthermore, the collision-induced dissociation (CID) behavior of the [M+NH4](+) ion of crispanone was also described.

  20. A Field-Portable Membrane Introduction Mass Spectrometer for Real-time Quantitation and Spatial Mapping of Atmospheric and Aqueous Contaminants

    NASA Astrophysics Data System (ADS)

    Bell, Ryan J.; Davey, Nicholas G.; Martinsen, Morten; Collin-Hansen, Christian; Krogh, Erik T.; Gill, Christopher G.

    2015-02-01

    Environmental concentrations of volatile and semivolatile organic compounds (VOC/SVOCs) can vary dramatically in time and space under the influence of environmental conditions. In an industrial setting, multiple point and diffuse sources can contribute to fugitive emissions. Assessments and monitoring programs using periodic grab sampling provide limited information, often with delay times of days or weeks. We report the development and use of a novel, portable membrane introduction mass spectrometry (MIMS) system capable of resolving and quantifying VOC and SVOCs with high spatial and temporal resolution, in the field, in real-time. An electron impact ionization cylindrical ion trap mass spectrometer modified with a capillary hollow fiber polydimethylsiloxane membrane interface was used for continuous air and water sampling. Tandem mass spectrometry and selected ion monitoring scans performed in series allowed for the quantitation of target analytes, and full scan mode was used to survey for unexpected analytes. Predeployment and in-field external calibrations were combined with a continuously infused internal standard to enable real-time quantitation and monitor instrument performance. The system was operated in a moving vehicle with internet-linked data processing and storage. Software development to integrate MIMS and relevant meta-data for visualization and geospatial presentation in Google Earth is presented. Continuous quantitation enables the capture of transient events that may be missed or under-represented by traditional grab sampling strategies. Real-time geospatial maps of chemical concentration enable adaptive sampling and in-field decision support. Sample datasets presented in this work were collected in Northern Alberta in 2010-2012.

  1. Thin-layer chromatography/direct analysis in real time time-of-flight mass spectrometry and isotope dilution to analyze organophosphorus insecticides in fatty foods.

    PubMed

    Kiguchi, Osamu; Oka, Kazuko; Tamada, Masafumi; Kobayashi, Takashi; Onodera, Jun

    2014-11-28

    To assess food safety emergencies caused by highly hazardous chemical-tainted foods, simultaneous analysis of organophosphorus insecticides in fatty foods such as precooked foods was conducted using thin-layer chromatography/direct analysis in real time time-of-flight mass spectrometry (TLC/DART-TOFMS) and isotope dilution technique. Polar (methamidophos and acephate) and nonpolar organophosphorus insecticides (fenitrothion, diazinon, and EPN) were studied. Experiments to ascertain chromatographic patterns using TLC/DART-TOFMS reveal that it was more useful than GC/MS or GC/MS/MS for the simultaneous analyses of polar and nonpolar pesticides, while obviating the addition of a protective agent for tailing effects of polar pesticides. Lower helium gas temperature (260°C) for DART-TOFMS was suitable for the simultaneous analysis of target pesticides. Linearities were achieved respectively at a lower standard concentration range (0.05-5 μg) for diazinon and EPN and at a higher standard concentration range (2.5-25 μg) for methamidophos, acephate, and fenitrothion. Their respective coefficients of determination were ≥ 0.9989 and ≥ 0.9959. A few higher repeatabilities (RSDs) for diazinon and EPN were found (>20%), although isotope dilution technique was used. Application to the HPTLC plate without an automatic TLC sampler might be inferred as a cause of their higher RSDs. Detection limits were estimated in the higher picogram range for diazinon and EPN, and in the lower nanogram range for methamidophos, acephate, and fenitrothion. Aside from methamidophos, recovery results (n=3) obtained using a highly insecticide-tainted fatty food (dumpling) and raw food (grapefruit) samples (10mg/kg) using TLC/DART-TOFMS with both complex and simpler cleanups were not as susceptible to matrix effects (95-121%; RSD, 1.3-14%) as those using GC/MS/MS (102-117%; RSD, 0.4-8.5%), although dumpling samples using GC/MS were remarkably susceptible to matrix effects. The coupled method of

  2. Quantitative real-time monitoring of multi-elements in airborne particulates by direct introduction into an inductively coupled plasma mass spectrometer

    NASA Astrophysics Data System (ADS)

    Suzuki, Yoshinari; Sato, Hikaru; Hiyoshi, Katsuhiro; Furuta, Naoki

    2012-10-01

    A new calibration system for real-time determination of trace elements in airborne particulates was developed. Airborne particulates were directly introduced into an inductively coupled plasma mass spectrometer, and the concentrations of 15 trace elements were determined by means of an external calibration method. External standard solutions were nebulized by an ultrasonic nebulizer (USN) coupled with a desolvation system, and the resulting aerosol was introduced into the plasma. The efficiency of sample introduction via the USN was calculated by two methods: (1) the introduction of a Cr standard solution via the USN was compared with introduction of a Cr(CO)6 standard gas via a standard gas generator and (2) the aerosol generated by the USN was trapped on filters and then analyzed. The Cr introduction efficiencies obtained by the two methods were the same, and the introduction efficiencies of the other elements were equal to the introduction efficiency of Cr. Our results indicated that our calibration method for introduction efficiency worked well for the 15 elements (Ti, V, Cr, Mn, Co, Ni, Cu, Zn, As, Mo, Sn, Sb, Ba, Tl and Pb). The real-time data and the filter-collection data agreed well for elements with low-melting oxides (V, Co, As, Mo, Sb, Tl, and Pb). In contrast, the real-time data were smaller than the filter-collection data for elements with high-melting oxides (Ti, Cr, Mn, Ni, Cu, Zn, Sn, and Ba). This result implies that the oxides of these 8 elements were not completely fused, vaporized, atomized, and ionized in the initial radiation zone of the inductively coupled plasma. However, quantitative real-time monitoring can be realized after correction for the element recoveries which can be calculated from the ratio of real-time data/filter-collection data.

  3. Real time analysis under EDS

    NASA Astrophysics Data System (ADS)

    Schneberk, D.

    1985-07-01

    The analysis component of the Enrichment Diagnostic System (EDS) developed for the Atomic Vapor Laser Isotope Separation Program (AVLIS) at Lawrence Livermore National Laboratory (LLNL) is described. Four different types of analysis are performed on data acquired through EDS: (1) absorption spectroscopy on laser-generated spectral lines, (2) mass spectrometer analysis, (3) general purpose waveform analysis, and (4) separation performance calculations. The information produced from this data includes: measures of particle density and velocity, partial pressures of residual gases, and overall measures of isotope enrichment. The analysis component supports a variety of real-time modeling tasks, a means for broadcasting data to other nodes, and a great degree of flexibility for tailoring computations to the exact needs of the process. A particular data base structure and program flow is common to all types of analysis. Key elements of the analysis component are: (1) a fast access data base which can configure all types of analysis, (2) a selected set of analysis routines, (3) a general purpose data manipulation and graphics package for the results of real time analysis.

  4. Real time analysis under EDS

    SciTech Connect

    Schneberk, D.

    1985-07-01

    This paper describes the analysis component of the Enrichment Diagnostic System (EDS) developed for the Atomic Vapor Laser Isotope Separation Program (AVLIS) at Lawrence Livermore National Laboratory (LLNL). Four different types of analysis are performed on data acquired through EDS: (1) absorption spectroscopy on laser-generated spectral lines, (2) mass spectrometer analysis, (3) general purpose waveform analysis, and (4) separation performance calculations. The information produced from this data includes: measures of particle density and velocity, partial pressures of residual gases, and overall measures of isotope enrichment. The analysis component supports a variety of real-time modeling tasks, a means for broadcasting data to other nodes, and a great degree of flexibility for tailoring computations to the exact needs of the process. A particular data base structure and program flow is common to all types of analysis. Key elements of the analysis component are: (1) a fast access data base which can configure all types of analysis, (2) a selected set of analysis routines, (3) a general purpose data manipulation and graphics package for the results of real time analysis. Each of these components are described with an emphasis upon how each contributes to overall system capability. 3 figs.

  5. Detection of polydimethylsiloxanes transferred from silicone-coated parchment paper to baked goods using direct analysis in real time mass spectrometry.

    PubMed

    Jakob, Andreas; Crawford, Elizabeth A; Gross, Jürgen H

    2016-04-01

    The non-stick properties of parchment papers are achieved by polydimethylsiloxane (PDMS) coatings. During baking, PDMS can thus be extracted from the silicone-coated parchment into the baked goods. Positive-ion direct analysis in real time (DART) mass spectrometry (MS) is highly efficient for the analysis of PDMS. A DART-SVP source was coupled to a quadrupole-time-of-flight mass spectrometer to detect PDMS on the contact surface of baked goods after use of silicone-coated parchment papers. DART spectra from the bottom surface of baked cookies and pizzas exhibited signals because of PDMS ions of the general formula [(C2H6SiO)n  + NH4 ](+) in the m/z 800-1900 range.

  6. Fast analysis of glycosides based on HKUST-1-coated monolith solid-phase microextraction and direct analysis in real-time mass spectrometry.

    PubMed

    Li, Xianjiang; Wang, Xin; Ma, Wen; Ai, Wanpeng; Bai, Yu; Ding, Li; Liu, Huwei

    2017-04-01

    Glycosides are a kind of highly important natural aromatic precursors in tobacco leaves. In this study, a novel HKUST-1-coated monolith dip-it sampler was designed for the fast and sensitive analysis of trace glycosides using direct analysis in real-time mass spectrometry. This device was prepared in two steps: in situ polymerization of monolith in a glass capillary of dip-it and layer-by-layer growth of HKUST-1 on the surface of monolith. Sufficient extraction was realized by immersing the tip to solution and in situ desorption was carried out by plasma direct analysis in real time. Compared with traditional solid-phase microextraction protocols, sample desorption was not needed anymore, and only extraction conditions were needed to be optimized in this method, including the gas temperature of direct analysis in real time, extraction time, and CH3 COONH4 additive concentration. This method enabled the simultaneous detection of six kinds of glycosides with the limits of detection of 0.02-0.05 μg/mL and the linear ranges covering two orders of magnitude with the limits of quantitation of 0.05-0.1 μg/mL. Moreover, the developed method was applied for the glycosides analysis of three tobacco samples, which only took about 2 s for every sample.

  7. Intact Endogenous Metabolite Analysis of Mice Liver by Probe Electrospray Ionization/Triple Quadrupole Tandem Mass Spectrometry and Its Preliminary Application to in Vivo Real-Time Analysis.

    PubMed

    Zaitsu, Kei; Hayashi, Yumi; Murata, Tasuku; Ohara, Tomomi; Nakagiri, Kenta; Kusano, Maiko; Nakajima, Hiroki; Nakajima, Tamie; Ishikawa, Tetsuya; Tsuchihashi, Hitoshi; Ishii, Akira

    2016-04-05

    Probe electrospray ionization (PESI) is a recently developed ionization technique that enables the direct detection of endogenous compounds like metabolites without sample preparation. In this study, we have demonstrated the first combination use of PESI with triple quadrupole tandem mass spectrometry (MS/MS), which was then applied to intact endogenous metabolite analysis of mice liver, achieving detection of 26 metabolites including amino acids, organic acids, and sugars. To investigate its practicality, metabolic profiles of control and CCl4-induced acute hepatic injury mouse model were measured by the developed method. Results showed clear separation of the two groups in score plots of principal component analysis and identified taurine as the primary contributor to group separation. The results were further validated by the established gas chromatography/MS/MS method, demonstrating the present method's usefulness. In addition, we preliminarily applied the method to real-time analysis of an intact liver of a living mouse. We successfully achieved monitoring of the real-time changes of two tricarboxylic acid cycle intermediates, α-ketoglutaric acid and fumaric acid, in the liver immediately after pyruvic acid injection via a cannulated tube to the portal vein. The present method achieved an intact analysis of metabolites in liver without sample preparation, and it also demonstrates future possibility to establish in vivo real-time metabolome analysis of living animals by PESI/MS/MS.

  8. Continuous determination of fine particulate matter mass in the Salt Lake City Environmental Monitoring project: a comparison of real-time and conventional TEOM monitor results.

    PubMed

    Long, Russell W; Eatough, Norman L; Eatough, Delbert J; Meyer, Michael B; Wilson, William E

    2005-12-01

    Fine particulate matter (PM2.5) mass was determined on a continuous basis at the Salt Lake City Environmental Protection Agency Environmental Monitoring for Public Awareness and Community Tracking monitoring site in Salt Lake City, UT, using three different monitoring techniques. Hourly averaged PM2.5 mass data were collected during two sampling periods (summer 2000 and winter 2002) using a real-time total ambient mass sampler (RAMS), sample equilibration system (SES)-tapered element oscillating microbalance (TEOM), and conventional TEOM monitor. This paper compares the results obtained from the various monitoring systems, which differ in their treatment of semivolatile material (SVM; particle-bound water, semivolatile ammonium nitrate, and semivolatile organic compounds). PM2.5 mass results obtained by the RAMS were consistently higher than those obtained by the SES-TEOM and conventional TEOM monitors because of the RAMS ability to measure semivolatile ammonium nitrate and semivolatile organic material but not particle-bound water. The SES-TEOM monitoring system was able to account for an average of 28% of the SVM, whereas the conventional TEOM monitor loses essentially all of the SVM from the single filter during sampling. Occasional mass readings by the various TEOM monitors that are higher than RAMS results may reflect particle-bound water, which, under some conditions, is measured by the TEOM but not the RAMS.

  9. Rapid fingerprinting of sterols and related compounds in vegetable and animal oils and phytosterol enriched- margarines by transmission mode direct analysis in real time mass spectrometry.

    PubMed

    Alberici, Rosana M; Fernandes, Gabriel D; Porcari, Andréia M; Eberlin, Marcos N; Barrera-Arellano, Daniel; Fernández, Facundo M

    2016-11-15

    Plant-derived sterols, often referred to as phytosterols, are important constituents of plant membranes where they assist in maintaining phospholipid bilayer stability. Consumption of phytosterols has been suggested to positively affect human health by reducing cholesterol levels in blood via inhibition of its absorption in the small intestine, thus protecting against heart attack and stroke. Sterols are challenging analytes for mass spectrometry, since their low polarity makes them difficult to ionize by both electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI), typically requiring derivatization steps to overcome their low ionization efficiencies. We present a fast and reliable method to characterize the composition of phytosterols in vegetable oils and enriched margarines. The method requires no derivatization steps or sample extraction procedures thanks to the use of transmission mode direct analysis in real time mass spectrometry (TM-DART-MS).

  10. Coupling of nanoflow liquid chromatography to matrix-assisted laser desorption/ionization mass spectrometry: real-time liquid chromatography run mapping on a MALDI plate.

    PubMed

    Nägele, Edgar; Vollmer, Martin

    2004-01-01

    The major obstacle in the use of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) instruments in the analysis of complex proteome samples is the lack of a direct coupling of a highly resolving separation technique with the mass spectrometer itself. To overcome this drawback, a spotting device for capillary and nanoflow liquid chromatography (LC) with a special liquid deposition principle for lowest volumes was developed. The instrument is able to perform MALDI spotting in real time in order to deposit the LC run on the MALDI plate, and therefore couples the high resolution power of nano-RP-HPLC separation directly with MALDI-MS. This work describes the development and optimization of a method for spotting with online matrix addition, and illustrates its use in the analysis of a complex proteome sample.

  11. Real Time Baseball Database

    NASA Astrophysics Data System (ADS)

    Fukue, Yasuhiro

    The author describes the system outline, features and operations of "Nikkan Sports Realtime Basaball Database" which was developed and operated by Nikkan Sports Shimbun, K. K. The system enables to input numerical data of professional baseball games as they proceed simultaneously, and execute data updating at realtime, just-in-time. Other than serving as supporting tool for prepareing newspapers it is also available for broadcasting media, general users through NTT dial Q2 and others.

  12. Real-time detection of common microbial volatile organic compounds from medically important fungi by Selected Ion Flow Tube-Mass Spectrometry (SIFT-MS).

    PubMed

    Scotter, Jennifer M; Langford, Vaughan S; Wilson, Paul F; McEwan, Murray J; Chambers, Stephen T

    2005-11-01

    We describe a new method, Selected Ion Flow Tube-Mass Spectrometry (SIFT-MS) for the rapid and sensitive real-time detection and quantification of volatile organic compounds from medically important fungi, grown on a range of laboratory media. SIFT-MS utilises the chemical ionisation reactions of mass-selected ions to characterise volatile organic compounds (VOCs) that are produced as metabolites from fungi. This technique has the distinct advantage over others in that it readily detects low molecular weight, reactive volatiles, and allows for real-time, quantitative monitoring. The fungi examined in this study were Aspergillus flavus, Aspergillus fumigatus, Candida albicans, Mucor racemosus, Fusarium solani, and Cryptococcus neoformans grown on or in malt extract agar, Columbia agar, Sabouraud's dextrose agar, blood agar, and brain-heart infusion broth. Common metabolites (ethanol, methanol, acetone, acetaldehyde, methanethiol, and crotonaldehyde) were detected and quantified. We found the fingerprint of volatiles, in terms of presence and quantity of volatiles to be strongly dependent on the culture medium, both in terms of variety and quantity of volatiles produced, but may form the basis for species specific identification of medically important fungi.

  13. Real-time flutter identification

    NASA Technical Reports Server (NTRS)

    Roy, R.; Walker, R.

    1985-01-01

    The techniques and a FORTRAN 77 MOdal Parameter IDentification (MOPID) computer program developed for identification of the frequencies and damping ratios of multiple flutter modes in real time are documented. Physically meaningful model parameterization was combined with state of the art recursive identification techniques and applied to the problem of real time flutter mode monitoring. The performance of the algorithm in terms of convergence speed and parameter estimation error is demonstrated for several simulated data cases, and the results of actual flight data analysis from two different vehicles are presented. It is indicated that the algorithm is capable of real time monitoring of aircraft flutter characteristics with a high degree of reliability.

  14. Real-time vision systems

    SciTech Connect

    Johnson, R.; Hernandez, J.E.; Lu, Shin-yee

    1994-11-15

    Many industrial and defence applications require an ability to make instantaneous decisions based on sensor input of a time varying process. Such systems are referred to as `real-time systems` because they process and act on data as it occurs in time. When a vision sensor is used in a real-time system, the processing demands can be quite substantial, with typical data rates of 10-20 million samples per second. A real-time Machine Vision Laboratory (MVL) was established in FY94 to extend our years of experience in developing computer vision algorithms to include the development and implementation of real-time vision systems. The laboratory is equipped with a variety of hardware components, including Datacube image acquisition and processing boards, a Sun workstation, and several different types of CCD cameras, including monochrome and color area cameras and analog and digital line-scan cameras. The equipment is reconfigurable for prototyping different applications. This facility has been used to support several programs at LLNL, including O Division`s Peacemaker and Deadeye Projects as well as the CRADA with the U.S. Textile Industry, CAFE (Computer Aided Fabric Inspection). To date, we have successfully demonstrated several real-time applications: bullet tracking, stereo tracking and ranging, and web inspection. This work has been documented in the ongoing development of a real-time software library.

  15. Real-Time Analysis of Individual Airborne Microparticles Using Laser Ablation Mass Spectroscopy and Genetically Trained Neural Networks

    SciTech Connect

    Parker, E.P.; Rosenthal, S.E.; Trahan, M.W.; Wagner, J.S.

    1999-01-22

    We are developing a method for analysis of airborne microparticles based on laser ablation of individual molecules in an ion trap mass spectrometer. Airborne particles enter the spectrometer through a differentially-pumped inlet, are detected by light scattered from two CW laser beams, and sampled by a pulsed excimer laser as they pass through the center of the ion trap electrodes. After the laser pulse, the stored ions are separated by conventional ion trap methods. The mass spectra are then analyzed using genetically-trained neural networks (NNs). A number of mass spectra are averaged to obtain training cases which contain a recognizable spectral signature. Averaged spectra for a bacteria and a non-bacteria are shown to the NNs, the response evaluated, and the weights of the connections between neurodes adjusted by a Genetic Algorithm (GA) such that the output from the NN ranges from 0 for non-bacteria to 1 for bacteria. This process is iterated until the population of the GA converges or satisfies predetermined stopping criteria. Using this type of bipolar training we have obtained generalizing NNs able to distinguish five new bacteria from five new non-bacteria, none of which were used in training the NN.

  16. Direct analysis in real time - high resolution mass spectrometry (DART-HRMS): a high throughput strategy for identification and quantification of anabolic steroid esters.

    PubMed

    Doué, Mickael; Dervilly-Pinel, Gaud; Pouponneau, Karinne; Monteau, Fabrice; Le Bizec, Bruno

    2015-07-01

    High throughput screening is essential for doping, forensic, and food safety laboratories. While hyphenated chromatography-mass spectrometry (MS) remains the approach of choice, recent ambient MS techniques, such as direct analysis in real time (DART), offer more rapid and more versatile strategies and thus gain in popularity. In this study, the potential of DART hyphenated with Orbitrap-MS for fast identification and quantification of 21 anabolic steroid esters has been evaluated. Direct analysis in high resolution scan mode allowed steroid esters screening by accurate mass measurement (Resolution = 60 000 and mass error < 3 ppm). Steroid esters identification was further supported by collision-induced dissociation (CID) experiments through the generation of two additional ions. Moreover, the use of labelled internal standards allowed quantitative data to be recovered based on isotopic dilution approach. Linearity (R(2)  > 0.99), dynamic range (from 1 to 1000 ng mL(-1) ), bias (<10%), sensitivity (1 ng mL(-1) ), repeatability and reproducibility (RSD < 20%) were evaluated as similar to those obtained with hyphenated chromatography-mass spectrometry techniques. This innovative high throughput approach was successfully applied for the characterization of oily commercial preparations, and thus fits the needs of the competent authorities in the fight against forbidden or counterfeited substances.

  17. Real-time software receiver

    NASA Technical Reports Server (NTRS)

    Ledvina, Brent M. (Inventor); Psiaki, Mark L. (Inventor); Powell, Steven P. (Inventor); Kintner, Jr., Paul M. (Inventor)

    2006-01-01

    A real-time software receiver that executes on a general purpose processor. The software receiver includes data acquisition and correlator modules that perform, in place of hardware correlation, baseband mixing and PRN code correlation using bit-wise parallelism.

  18. Real-time software receiver

    NASA Technical Reports Server (NTRS)

    Ledvina, Brent M. (Inventor); Psiaki, Mark L. (Inventor); Powell, Steven P. (Inventor); Kintner, Jr., Paul M. (Inventor)

    2007-01-01

    A real-time software receiver that executes on a general purpose processor. The software receiver includes data acquisition and correlator modules that perform, in place of hardware correlation, baseband mixing and PRN code correlation using bit-wise parallelism.

  19. Real Time Data System (RTDS)

    NASA Technical Reports Server (NTRS)

    Muratore, John F.

    1991-01-01

    Lessons learned from operational real time expert systems are examined. The basic system architecture is discussed. An expert system is any software that performs tasks to a standard that would normally require a human expert. An expert system implies knowledge contained in data rather than code. And an expert system implies the use of heuristics as well as algorithms. The 15 top lessons learned by the operation of a real time data system are presented.

  20. REFIR - The operational FutureVolc multi-parameter system providing a best estimate of mass eruption rate during ongoing eruptions in near real-time

    NASA Astrophysics Data System (ADS)

    Dürig, Tobias

    2016-04-01

    Volcanic ash injected into the atmosphere poses a serious threat for aviation. Forecasting the concentration of ash promptly requires detailed knowledge of eruption source parameters. However, monitoring an ongoing eruption and quantifying the mass flux in real-time is a considerable challenge. Due to the large uncertainties affecting present-day models, best estimates are often obtained by the application of integrated approaches. One example for this strategy is represented by the EU supersite project "FutureVolc" which aims to monitor eruptions of volcanoes in Iceland. A quasi-autonomous multi-parameter system, denoted "REFIR", has been developed. REFIR makes use of streaming data provided by a multitude of sensors, e.g. by C- and X-band radars, web-cam based plume height tracking systems, imaging ultra-violet and infrared cameras and electric field sensors. These observations are used with plume models that also consider the current local wind and other atmospheric conditions, and a best estimate of source parameters, including the mass eruption rate, is provided in near real-time (within a time interval of 5 minutes) as soon as an eruption has started. Since neither the time nor the location of the next Icelandic eruption is known the system has been developed with a guiding principle of maximum flexibility, and it can effortlessly be implemented elsewhere needing minimum adoption to local conditions. Moreover, it is designed to be easily upgraded, which allows future extension of the existing monitoring network, learning from new events, and incorporating new technologies and model improvements. Data-flow, features and integrated models within REFIR will be presented and strategies for implementing potential future research developments on ash plume dynamics will be discussed.

  1. An architecture for real time data acquisition and online signal processing for high throughput tandem mass spectrometry

    SciTech Connect

    Shah, Anuj R.; Jaitly, Navdeep; Zuljevic, Nino; Monroe, Matthew E.; Liyu, Andrei V.; Polpitiya, Ashoka D.; Adkins, Joshua N.; Belov, Mikhail E.; Anderson, Gordon A.; Smith, Richard D.; Gorton, Ian

    2010-12-09

    Independent, greedy collection of data events using simple heuristics results in massive over-sampling of the prominent data features in large-scale studies over what should be achievable through “intelligent,” online acquisition of such data. As a result, data generated are more aptly described as a collection of a large number of small experiments rather than a true large-scale experiment. Nevertheless, achieving “intelligent,” online control requires tight interplay between state-of-the-art, data-intensive computing infrastructure developments and analytical algorithms. In this paper, we propose a Software Architecture for Mass spectrometry-based Proteomics coupled with Liquid chromatography Experiments (SAMPLE) to develop an “intelligent” online control and analysis system to significantly enhance the information content from each sensor (in this case, a mass spectrometer). Using online analysis of data events as they are collected and decision theory to optimize the collection of events during an experiment, we aim to maximize the information content generated during an experiment by the use of pre-existing knowledge to optimize the dynamic collection of events.

  2. Enzymatic Hydrolysis of Polyester Thin Films: Real-Time Analysis of Film Mass Changes and Dissipation Dynamics.

    PubMed

    Zumstein, Michael Thomas; Kohler, Hans-Peter E; McNeill, Kristopher; Sander, Michael

    2016-01-05

    Cleavage of ester bonds by extracellular microbial hydrolases is considered a key step during the breakdown of biodegradable polyester materials in natural and engineered systems. Here we present a novel analytical approach for simultaneous detection of changes in the masses and rigidities of polyester thin films during enzymatic hydrolysis using a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D). In experiments with poly(butylene succinate) (PBS) and the lipase of Rhizopus oryzae (RoL), we detected complete hydrolysis of PBS thin films at pH 5 and 40 °C that proceeded through soft and water-rich film intermediates. Increasing the temperature from 20 to 40 °C resulted in a larger increase of the enzymatic hydrolysis rate of PBS than of nonpolymeric dibutyl adipate. This finding was ascribed to elevated accessibility of ester bonds to the catalytic site of RoL due to increasing polyester chain mobility. When the pH of the solution was changed from 5 to 7, initial hydrolysis rates were little affected, while a softer film intermediate that lead to incomplete film hydrolysis was formed. Hydrolysis dynamics of PBS, poly(butylene adipate), poly(lactic acid), and poly(ethylene terephthalate) in assays with RoL showed distinct differences that we attribute to differences in the polyester structure.

  3. Real-time quadrupole mass spectrometer analysis of gas in boreholefluid samples acquired using the U-Tube sampling methodology

    SciTech Connect

    Freifeld, Barry M.; Trautz, Robert C.

    2006-01-11

    Sampling of fluids in deep boreholes is challenging becauseof the necessity to minimize external contamination and maintain sampleintegrity during recovery. The U-tube sampling methodology was developedto collect large volume, multiphase samples at in situ pressures. As apermanent or semi-permanent installation, the U-tube can be used forrapidly acquiring multiple samples or it may be installed for long-termmonitoring applications. The U-tube was first deployed in Liberty County,TX to monitor crosswell CO2 injection as part of the Frio CO2sequestration experiment. Analysis of gases (dissolved or separate phase)was performed in the field using a quadrupole mass spectrometer, whichserved as the basis for determining the arrival of the CO2 plume. Thepresence of oxygen and argon in elevated concentrations, along withreduced methane concentration, indicate sample alteration caused by theintroduction of surface fluids during borehole completion. Despiteproducing the well to eliminate non-native fluids, measurementsdemonstrate that contamination persists until the immiscible CO2injection swept formation fluid into the observationwellbore.

  4. Proton transfer reaction mass spectrometry for the sensitive and rapid real-time detection of solid high explosives in air and water.

    PubMed

    Jürschik, S; Sulzer, P; Petersson, F; Mayhew, C A; Jordan, A; Agarwal, B; Haidacher, S; Seehauser, H; Becker, K; Märk, T D

    2010-12-01

    Relying on recent developments in proton transfer reaction mass spectrometry (PTR-MS), we demonstrate here the capability of detecting solid explosives in air and in water in real time. Two different proton transfer reaction mass spectrometers have been used in this study. One is the PTR-TOF 8000, which has an enhanced mass resolution (m/Δm up to 8,000) and high sensitivity (~50 cps/ppbv). The second is the high-sensitivity PTR-MS, which has an improved limit of detection of about several hundreds of parts per quadrillion by volume and is coupled with a direct aqueous injection device. These instruments have been successfully used to identify and monitor the solid explosive 2,4,6-trinitrotoluene (TNT) by analysing on the one hand the headspace above small quantities of samples at room temperature and from trace quantities not visible to the naked eye placed on surfaces (also demonstrating the usefulness of a simple pre-concentration and thermal desorption technique) and by analysing on the other hand trace compounds in water down to a level of about 100 pptw. The ability to identify even minute amounts of threat compounds, such as explosives, particularly within a complex chemical environment, is vital to the fight against crime and terrorism and is of paramount importance for the appraisal of the fate and harmful effects of TNT at marine ammunition dumping sites and the detection of buried antipersonnel and antitank landmines.

  5. (Un)targeted Scanning of Locks of Hair for Drugs of Abuse by Direct Analysis in Real Time-High-Resolution Mass Spectrometry.

    PubMed

    Duvivier, Wilco F; van Putten, Marc R; van Beek, Teris A; Nielen, Michel W F

    2016-02-16

    Forensic hair evidence can be used to obtain retrospective timelines of drug use by analysis of hair segments. However, this is a laborious and time-consuming process, and mass spectrometric (MS) imaging techniques, which show great potential for single-hair targeted analysis, are less useful due to differences in hair growth rate between individual hairs. As an alternative, a fast untargeted analysis method was developed that uses direct analysis in real time-high-resolution mass spectrometry (DART-HRMS) to longitudinally scan intact locks of hair without extensive sample preparation or segmentation. The hair scan method was validated for cocaine against an accredited liquid chromatography/tandem mass spectrometry (LC/MS/MS) method. The detection limit for cocaine in hair was found to comply with the cutoff value of 0.5 ng/mg recommended by the Society of Hair Testing; that is, the DART hair scan method is amenable to forensic cases. Under DART conditions, no significant thermal degradation of cocaine occurred. The standard DART spot size of 5.1 ± 1.1 mm could be improved to 3.3 ± 1.0 mm, corresponding to approximately 10 days of hair growth, by using a high spatial resolution exit cone. By use of data-dependent product ion scans, multiple drugs of abuse could be detected in a single drug user hair scan with confirmation of identity by both exact mass and MS/HRMS fragmentation patterns. Furthermore, full-scan high-resolution data were retrospectively interrogated versus a list of more than 100 compounds and revealed additional hits and temporal profiles in good correlation with reported drug use.

  6. Dependable Real-Time Systems

    DTIC Science & Technology

    1991-09-30

    and F. Wang, "On thle Competitiveness of On-Line Real-Time Task Sc~eduling," to appear. Proc. Icai - Time Systemns Symposium, Dec 1991. 6. Biyabaiii, S...Stankovic, and K. Ramrnamritham, "System Support for lRal-’Vi111C Al: A Spring Project Perspective," Workshop on Real-Time .A1, ICAI ., August 198!). 29...Informatics, Computer S,,iety ,f India , t,, aptpear. 41 . Shilh, C. and J. A. Stankovic, "Distributed Deadlock Detection in Ada IRuntinv En vi- ronments," TRI

  7. Towards monitoring real-time cellular response using an integrated microfluidics-matrix assisted laser desorption ionisation/nanoelectrospray ionisation-ion mobility-mass spectrometry platform.

    PubMed

    Enders, J R; Marasco, C C; Kole, A; Nguyen, B; Sevugarajan, S; Seale, K T; Wikswo, J P; McLean, J A

    2010-11-01

    The combination of microfluidic cell trapping devices with ion mobility-mass spectrometry offers the potential for elucidating in real time the dynamic responses of small populations of cells to paracrine signals, changes in metabolite levels and delivery of drugs and toxins. Preliminary experiments examining peptides in methanol and recording the interactions of yeast and Jurkat cells with their superfusate have identified instrumental set-up and control parameters and online desalting procedures. Numerous initial experiments demonstrate and validate this new instrumental platform. Future outlooks and potential applications are addressed, specifically how this instrumentation may be used for fully automated systems biology studies of the significantly interdependent, dynamic internal workings of cellular metabolic and signalling pathways.

  8. Transmission-mode direct analysis in real time and desorption electrospray ionization mass spectrometry of insecticide-treated bednets for malaria control.

    PubMed

    Pérez, José J; Harris, Glenn A; Chipuk, Joseph E; Brodbelt, Jennifer S; Green, Michael D; Hampton, Christina Y; Fernández, Facundo M

    2010-04-01

    Transmission-mode direct analysis in real time (TM-DART) is presented as an alternative sampling strategy to traditional methods of sample introduction for DART MS analysis. A custom-designed sample holder was fabricated to rapidly and reproducibly position insecticide-treated nets normal to the ionizing metastable gas stream, enabling transmission of desorbed analyte ions through the holder cavity and into the MS. Introduction of the sample at this fixed geometry eliminates the need for optimizing sample position and allows spectra based on factors such as metastable gas temperature and flow to be systematically evaluated. The results presented here, supported by computational fluid dynamic simulations, demonstrate the effects of these factors on the resulting mass spectra and the potential of this sampling strategy to be used for qualitative and quantitative analyses. Transmission-mode desorption electrospray ionization (TM-DESI) experiments on similar insecticide-treated nets were performed for comparison purposes.

  9. HEVC real-time decoding

    NASA Astrophysics Data System (ADS)

    Bross, Benjamin; Alvarez-Mesa, Mauricio; George, Valeri; Chi, Chi Ching; Mayer, Tobias; Juurlink, Ben; Schierl, Thomas

    2013-09-01

    The new High Efficiency Video Coding Standard (HEVC) was finalized in January 2013. Compared to its predecessor H.264 / MPEG4-AVC, this new international standard is able to reduce the bitrate by 50% for the same subjective video quality. This paper investigates decoder optimizations that are needed to achieve HEVC real-time software decoding on a mobile processor. It is shown that HEVC real-time decoding up to high definition video is feasible using instruction extensions of the processor while decoding 4K ultra high definition video in real-time requires additional parallel processing. For parallel processing, a picture-level parallel approach has been chosen because it is generic and does not require bitstreams with special indication.

  10. A Minimal Fragmentation Approach to Real Time Aerosol Mass Spectrometry: A New Tool for Detailed Laboratory Studies of Organic Aerosol Aging

    NASA Astrophysics Data System (ADS)

    Campuzano-Jost, P.; Hanna, S.; Simpson, E.; Robb, D.; Blades, M. W.; Hepburn, J. W.; Bertram, A. K.

    2005-12-01

    have used almost exclusively a single photon energy (10.5 eV) to ionize all molecules. We have constructed a new real time aerosol mass spectrometer, based on the designs of Su et al. (2004) & Sykes et al. (2002), which incorporates a new, high-powered, fully tunable VUV source (115-190 nm, 6.5-11 eV ionization energy). This enables us to ionize quantitatively with little to no fragmentation almost all atmospherically relevant organic compounds. Coupled with an ion trap where the primary ions can be stored for further structural analysis, this instrument promises to be both universal and sensitive enough to allow for detailed studies of SOA formation as well as the aging of mixed aerosols. References: Allan, J.D., J.L. Jimenez, et al. (2003)."Quantitative sampling using an Aerodyne aerosol mass spectrometer: 1.Techniques of data interpretation and error analysis" J.Geophys.Res. 108(D9) Nash, D.G., X.F. Liu, et al. (2005)."Aerosol particle mass spectrometry with low photon energy laser ionization" Inter.J.Mass Spec. 241(2-3):89 Oktem,B., M.P. Tolocka, et al. (2004)."On-line analysis of organic components in fine and ultrafine particles by photoionization aerosol mass spectrometry" Anal.Chem. 76(2):253 Su, Y.X., M.F. Sipin, et al. (2004)."Development and characterization of an aerosol time-of-flight mass spectrometer with increased detection efficiency" Anal.Chem. 76(3):712 Sykes, D.C., E. Woods, et al. (2002)."Thermal vaporization-vacuum ultraviolet laser ionization time-of-flight mass spectrometry of single aerosol particles" Anal.Chem. 74(9):2048

  11. Rapid control of Chinese star anise fruits and teas for neurotoxic anisatin by Direct Analysis in Real Time high resolution mass spectrometry.

    PubMed

    Shen, Yao; van Beek, Teris A; Claassen, Frank W; Zuilhof, Han; Chen, Bo; Nielen, Michel W F

    2012-10-12

    After ingestion, products containing Chinese star anise (Illicium verum) contaminated or adulterated with Japanese star anise (Illicium anisatum) or other Illicium species, can cause epilepsy, hallucinations, and nausea due to the rare neurotoxic sesquiterpene dilactone anisatin that is present in Japanese star anise. Thus a rapid, simple and unambiguous method for distinguishing between the morphologically similar Chinese star anise and toxic Japanese star anise is important for food safety issues. Direct Analysis in Real Time (DART) ambient ionisation coupled with orbitrap high resolution mass spectrometry allowed the recording of mass spectra of anisatin in solid star anise fruits in seconds without any prior sample pretreatment. Spectra could be obtained in both positive ([M+NH(4)](+) at m/z 346.1496, C(15)H(24)NO(8)) and negative mode ([M-H](-) at m/z 327.1074, C(15)H(19)O(8)) and gave the same outcome provided a mass resolution of at least 27,000 is available. The anisatin signal was typically >1000 times larger in Japanese star anise than in Chinese star anise thus allowing an unequivocal qualitative determination. Herbal teas containing star anise fragments too small to be visually recognised, could be analysed by preparing a tea in 6 min and subsequently sampling ∼2 μL of tea on a glass rod. None of the 8 investigated retail teas contained significant quantities of anisatin. Spiking a complex herbal tea containing Chinese star anise with an equally concentrated tea prepared from Japanese star anise provided a linear calibration curve (R(2) ≥ 0.995) after normalising on a native constituent of Chinese star anise (standard addition method). This showed that adulteration down to 1% (w/w) is still measurable. Compared with existing PCR, TLC, GC-MS and HPLC-ESI-MS/MS procedures, the proposed DART-HRMS procedure is faster and simpler and moreover measures the actual biotoxin.

  12. Direct peel monitoring of xenobiotics in fruit by direct analysis in real time coupled to a linear quadrupole ion trap-orbitrap mass spectrometer.

    PubMed

    Farré, Marinella; Picó, Yolanda; Barceló, Damià

    2013-03-05

    Study of xenobiotics present in fruit peel by exposing it (without any pretreatment) to direct analysis in real time coupled to a high-resolution orbitrap mass spectrometer (DART-HRMS) is reported for the first time. Variables such as DART gas heater temperature and pressure, source-to-MS distance, and sample velocity are investigated. The analysis of one sample by DART-MS lasts ca. 1 min, and the benefits of both high-resolution and tandem mass spectrometry to elucidate nontarget or unknown compounds are combined. Identification of postharvest fungicides, antioxidants, and sugars in fruit peel is performed in the positive ion mode. A possible elemental formula is suggested for marker components. The lowest imazalil concentration that could be detected by this system is 1 ng (equivalent to a concentration of ca. 300 μg kg(-1)), which is well below the maximum residue limit. For oranges and apples, direct peel exposition demonstrated good interday precision (within 20% for any concentration) and proper linearity (R(2) ≥ 0.99), with a dynamic range from 1 to 2500 ng for apple. A comparison of the results obtained using the direct peel screening DART-based method is made with those obtained by DART analysis of solvent extracts, as well as those obtained analyzing these extracts by ultrahigh-performance liquid chromatography orbitrap mass spectrometry (UHPLC-Orbitrap). The results are in good agreement. Thus, the proposed method proves to be quantitatively accurate with indisputable identification specificity. As an independent method, the approach of direct scanning of peel is of high interest and of potential future within food analysis to guarantee safety, quality, and authenticity.

  13. Enhanced aerodynamic reach of vapor and aerosol sampling for real-time mass spectrometric detection using Venturi-assisted entrainment and ionization.

    PubMed

    Forbes, Thomas P; Staymates, Matthew

    2017-03-08

    Venturi-assisted ENTrainment and Ionization (VENTI) was developed, demonstrating efficient entrainment, collection, and transport of remotely sampled vapors, aerosols, and dust particulate for real-time mass spectrometry (MS) detection. Integrating the Venturi and Coandă effects at multiple locations generated flow and analyte transport from non-proximate locations and more importantly enhanced the aerodynamic reach at the point of collection. Transport through remote sampling probes up to 2.5 m in length was achieved with residence times on the order of 10(-2) s to 10(-1) s and Reynolds numbers on the order of 10(3) to 10(4). The Venturi-assisted entrainment successfully enhanced vapor collection and detection by greater than an order of magnitude at 20 cm stand-off (limit of simple suction). This enhancement is imperative, as simple suction restricts sampling to the immediate vicinity, requiring close proximity to the vapor source. In addition, the overall aerodynamic reach distance was increased by approximately 3-fold over simple suction under the investigated conditions. Enhanced aerodynamic reach was corroborated and observed with laser-light sheet flow visualization and schlieren imaging. Coupled with atmospheric pressure chemical ionization (APCI), the detection of a range of volatile chemical vapors; explosive vapors; explosive, narcotic, and mustard gas surrogate (methyl salicylate) aerosols; and explosive dust particulate was demonstrated. Continuous real-time Venturi-assisted monitoring of a large room (approximately 90 m(2) area, 570 m(3) volume) was demonstrated for a 60-min period without the remote sampling probe, exhibiting detection of chemical vapors and methyl salicylate at approximately 3 m stand-off distances within 2 min of exposure.

  14. Profiling and classification of French propolis by combined multivariate data analysis of planar chromatograms and scanning direct analysis in real time mass spectra.

    PubMed

    Chasset, Thibaut; Häbe, Tim T; Ristivojevic, Petar; Morlock, Gertrud E

    2016-09-23

    Quality control of propolis is challenging, as it is a complex natural mixture of compounds, and thus, very difficult to analyze and standardize. Shown on the example of 30 French propolis samples, a strategy for an improved quality control was demonstrated in which high-performance thin-layer chromatography (HPTLC) fingerprints were evaluated in combination with selected mass signals obtained by desorption-based scanning mass spectrometry (MS). The French propolis sample extracts were separated by a newly developed reversed phase (RP)-HPTLC method. The fingerprints obtained by two different detection modes, i.e. after (1) derivatization and fluorescence detection (FLD) at UV 366nm and (2) scanning direct analysis in real time (DART)-MS, were analyzed by multivariate data analysis. Thus, RP-HPTLC-FLD and RP-HPTLC-DART-MS fingerprints were explored and the best classification was obtained using both methods in combination with pattern recognition techniques, such as principal component analysis. All investigated French propolis samples were divided in two types and characteristic patterns were observed. Phenolic compounds such as caffeic acid, p-coumaric acid, chrysin, pinobanksin, pinobanksin-3-acetate, galangin, kaempferol, tectochrysin and pinocembrin were identified as characteristic marker compounds of French propolis samples. This study expanded the research on the European poplar type of propolis and confirmed the presence of two botanically different types of propolis, known as the blue and orange types.

  15. Prediction of acrylamide formation in biscuits based on fingerprint data generated by ambient ionization mass spectrometry employing direct analysis in real time (DART) ion source.

    PubMed

    Vaclavik, Lukas; Capuano, Edoardo; Gökmen, Vural; Hajslova, Jana

    2015-04-15

    The objective of this study is the evaluation of the potential of high-throughput direct analysis in real time-high resolution mass spectrometry (DART-HRMS) fingerprinting and multivariate regression analysis in prediction of the extent of acrylamide formation in biscuit samples prepared by various recipes and baking conditions. Information-rich mass spectral fingerprints were obtained by analysis of biscuit extracts for preparation of which aqueous methanol was used. The principal component analysis (PCA) of the acquired data revealed an apparent clustering of samples according to the extent of heat-treatment applied during the baking of the biscuits. The regression model for prediction of acrylamide in biscuits was obtained by partial least square regression (PLSR) analysis of the data matrix representing combined positive and negative ionization mode fingerprints. The model provided a least root mean square error of cross validation (RMSECV) equal to an acrylamide concentration of 5.4 μg kg(-1) and standard error of prediction (SEP) of 14.8 μg kg(-1). The results obtained indicate that this strategy can be used to accurately predict the amounts of acrylamide formed during baking of biscuits. Such rapid estimation of acrylamide concentration can become a useful tool in evaluation of the effectivity of processes aiming at mitigation of this food processing contaminant. However, the robustness this approach with respect to variability in the chemical composition of ingredients used for preparation of biscuits should be tested further.

  16. Sources and atmospheric processing of winter aerosols in Seoul, Korea: insights from real-time measurements using a high-resolution aerosol mass spectrometer

    NASA Astrophysics Data System (ADS)

    Kim, Hwajin; Zhang, Qi; Bae, Gwi-Nam; Kim, Jin Young; Bok Lee, Seung

    2017-02-01

    Highly time-resolved chemical characterization of nonrefractory submicrometer particulate matter (NR-PM1) was conducted in Seoul, the capital and largest metropolis of Korea, using an Aerodyne high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS). The measurements were performed during winter, when elevated particulate matter (PM) pollution events are often observed. This is the first time that detailed real-time aerosol measurement results have been reported from Seoul, Korea, and they reveal valuable insights into the sources and atmospheric processes that contribute to PM pollution in this region. The average concentration of submicron aerosol (PM1 = NR-PM1+ black carbon (BC)) was 27.5 µg m-3, and the total mass was dominated by organics (44 %), followed by nitrate (24 %) and sulfate (10 %). The average atomic ratios of oxygen to carbon (O / C), hydrogen to carbon (H / C), and nitrogen to carbon (N / C) of organic aerosols (OA) were 0.37, 1.79, and 0.018, respectively, which result in an average organic mass-to-carbon (OM / OC) ratio of 1.67. The concentrations (2.6-90.7 µg m-3) and composition of PM1 varied dynamically during the measurement period due to the influences of different meteorological conditions, emission sources, and air mass origins. Five distinct sources of OA were identified via positive matrix factorization (PMF) analysis of the HR-ToF-AMS data: vehicle emissions represented by a hydrocarbon-like OA factor (HOA, O / C = 0.06), cooking activities represented by a cooking OA factor (COA, O / C = 0.14), wood combustion represented by a biomass burning OA factor (BBOA, O / C = 0.34), and secondary organic aerosol (SOA) represented by a semivolatile oxygenated OA factor (SV-OOA, O / C = 0.56) and a low-volatility oxygenated OA factor (LV-OOA, O / C = 0.68). On average, primary OA (POA = HOA + COA + BBOA) accounted for 59 % the OA mass, whereas SV-OOA and LV-OOA contributed 15 and 26 %, respectively. Our results indicate that air

  17. Real Time Sonic Boom Display

    NASA Technical Reports Server (NTRS)

    Haering, Ed

    2014-01-01

    This presentation will provide general information about sonic boom mitigation technology to the public in order to supply information to potential partners and licensees. The technology is a combination of flight data, atmospheric data and terrain information implemented into a control room real time display for flight planning. This research is currently being performed and as such, any results and conclusions are ongoing.

  18. Real-time monitoring of fragrance release from cotton towels by low thermal mass gas chromatography using a longitudinally modulating cryogenic system for headspace sampling and injection.

    PubMed

    Haefliger, Olivier P; Jeckelmann, Nicolas; Ouali, Lahoussine; León, Géraldine

    2010-01-15

    An innovative headspace sampling and injection system for gas chromatography was designed using a longitudinally modulating cryogenic system mounted around the sampling loop of a two-position loop injector. The setup was hyphenated to a fast low thermal mass gas chromatograph, allowing transient concentrations of semivolatile analytes to be monitored in real time with a time resolution of 4.5 min. The performance of the instrument, and in particular its cryotrapping efficiency, was characterized using a mixture of long-chain alkanes, methyl esters, ethyl esters, and alcohols of different volatilities. The device was found to be ideally suited to the analysis of semivolatile compounds with boiling points ranging between 190 and 320 degrees C, which are typical for a majority of perfumery raw materials. The new instrument was successfully used to monitor the release of eight odorant compounds from cotton towels to which fabric softener had been applied that alternatively contained the fragrance in free form or in microencapsulated form. The analytical results, unprecedented in their level of precision and time resolution for such an application, evidenced the major impact of microencapsulation technology on the kinetics of fragrance release during the drying of the towels and on the triggering of additional fragrance release by applying mechanical stress to the fabric to rupture the microcapsule walls.

  19. Doping-assisted low-pressure photoionization mass spectrometry for the real-time detection of lung cancer-related volatile organic compounds.

    PubMed

    Li, Zhen; Xu, Ce; Shu, Jinian; Yang, Bo; Zou, Yao

    2017-04-01

    Real-time detection of lung cancer-related volatile organic compounds (VOCs) is a promising, non-intrusive technique for lung cancer (LC) prescreening. In this study, a novel method was designed to enhance the detection selectivity and sensitivity of LC-related polar VOCs by dichloromethane (CH2Cl2) doping-assisted low-pressure photoionization mass spectrometry (LPPI-MS). Compared with conventional LPPI-MS, CH2Cl2 doping-assisted LPPI-MS boosted the peak intensities of n-propanol, n-pentanal, acetone, and butyl acetate in nitrogen specifically by 53, 18, 16, and 43 times, respectively. The signal intensities of their daughter ions were inhibited or reduced. At relative humidity (RH) of 20%, the sensitivities of n-propanol, n-pentanal, acetone, and butyl acetate detection ranged from 116 to 452 counts/ppbv with a detection time of 10s and R(2) >0.99 for the linear calibration curves. The method was also applicable under higher RH levels of 50% and 90%. Breath samples obtained from 10 volunteers and spiked samples were investigated. Eight-fold enhancements in the signal intensities of polar VOCs were observed in the normal and spiked samples. These preliminary results demonstrate the efficacy of the dichloromethane doping-assisted LPPI technique for the detection of LC-related polar VOCs. Further studies are indispensible to illustrating the detailed mechanism and applying the technique to breath diagnosis.

  20. Cost Analysis of Implementing Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Plus Real-Time Antimicrobial Stewardship Intervention for Bloodstream Infections.

    PubMed

    Patel, Twisha S; Kaakeh, Rola; Nagel, Jerod L; Newton, Duane W; Stevenson, James G

    2017-01-01

    Studies evaluating rapid diagnostic testing plus stewardship intervention have consistently demonstrated improved clinical outcomes for patients with bloodstream infections. However, the cost of implementing new rapid diagnostic testing can be significant, and such testing usually does not generate additional revenue. There are minimal data evaluating the impact of adding matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for rapid organism identification and dedicating pharmacy stewardship personnel time on the total hospital costs. A cost analysis was performed utilizing patient data generated from the hospital cost accounting system and included additional costs of MALDI-TOF equipment, supplies and personnel, and dedicated pharmacist time for blood culture review and of making interventions to antimicrobial therapy. The cost analysis was performed from a hospital perspective for 3-month blocks before and after implementation of MALDI-TOF plus stewardship intervention. A total of 480 patients with bloodstream infections were included in the analysis: 247 in the preintervention group and 233 in the intervention group. Thirty-day mortality was significantly improved in the intervention group (12% versus 21%, P < 0.01), and the mean length of stay was reduced, although the difference was not statistically significant (13.0 ± 16.5 days versus 14.2 ± 16.7 days, P = 0.44). The total hospital cost per bloodstream infection was lower in the intervention group ($42,580 versus $45,019). Intensive care unit cost per bloodstream infection accounted for the largest share of the total costs in each group and was also lower in the intervention group ($10,833 versus $13,727). Implementing MALDI-TOF plus stewardship review and intervention decreased mortality for patients with bloodstream infections. Despite the additional costs of implementing MALDI-TOF and of dedicating pharmacy stewardship personnel time to interventions, the total

  1. Real Time Data Analysis Techniques

    NASA Astrophysics Data System (ADS)

    Silberberg, George G.

    1983-03-01

    By the early 1970s, classical photo-optical range instrumentation technology (as a means of gathering weapons' system performance data) had become a costly and inefficient process. Film costs were increasing due to soaring silver prices. Time required to process, read, and produce optical data was becoming unacceptable as a means of supporting weapon system development programs. NWC investigated the feasibility of utilizing Closed Circuit Television (CCTV) technology as an alternative solution for providing optical data. In 1978 a program entitled Metric Video (measurements from video images) was formulated at the Naval Weapons Center, China Lake, California. The purpose of this program was to provide timely data, to reduce the number of operating personnel, and to lower data acquisition costs. Some of the task elements for this program included a near real-time vector miss-distance system, a weapons scoring system, a velocity measuring system, a time-space position system, and a system to replace film cameras for gathering real-time engineering sequential data. These task elements and the development of special hardware and techniques to achieve real-time data will be discussed briefly in this paper.

  2. Multiple heart-cutting two dimensional liquid chromatography mass spectrometry: Towards real time determination of related impurities of bio-pharmaceuticals in salt based separation methods.

    PubMed

    Petersson, Patrik; Haselmann, Kim; Buckenmaier, Stephan

    2016-10-14

    Many of the chromatographic methods used in industry to determine related impurities in bio pharmaceuticals employ salt containing mobile phases. "Salty" mobile phases often provide superior chromatographic performance but are not compatible with mass spectrometry (MS) detection. Peak tracking necessary for method development is therefore often based on peak areas and the chemist's experience/intuition. In addition, MS characterization of impurities usually is done by offline fraction collection, which apart from being time consuming often suffers from poor recovery or the degradation of impurities collected. The recent development of multiple heart-cutting (MHC) two-dimensional liquid chromatography (2D-LC) provides a way to address these problems. This study shows how MHC 2D-LC-MS can be used to obtain almost real time MS data for bovine insulin related impurities present at low level (<0.03%). High quality MS spectra were obtained even for a first dimension using a mobile phase containing high concentrations of sodium, sulphate and phosphate. Thereby MHC 2D-LC-MS offers a possibility to eliminate the guesswork currently associated with peak tracking during method development. Furthermore, in contrast to current characterization methods involving fraction collection, solvent reduction/exchange etc., MS determination is done directly, which markedly shortens the workflow (from days to hours) and reduces the risk for poor recovery and degradation.

  3. Direct analysis in real time-mass spectrometry (DART-MS) for rapid qualitative screening of toxic glycols in glycerin-containing products.

    PubMed

    Self, Randy L

    2013-06-01

    In 2007, the United States Food and Drug Administration released guidance recommending testing of glycerin used in regulated consumer products, such as cough syrup preparations, toothpaste, and other pharmaceutical and food products, for the toxic compounds ethylene glycol and diethylene glycol. Regulatory laboratories routinely test glycerin, and products containing glycerin or related compounds for these toxic glycols, using an official gas chromatographic method, to ensure the safety of these products. The current work describes a companion technique to compliment this GC-FID method utilizing Orbitrap mass spectrometry with direct analysis in real time ionization to rapidly screen these samples qualitatively, with results in as little as five seconds, with no sample preparation required. This allows the more time and resource intensive method to be reserved for those rare cases when these compounds are detected, potentially greatly improving laboratory efficiency. The technique was evaluated for qualitative sensitivity and repeatability, and compared against the GC-FID method. The method appears to perform well against these metrics.

  4. Characterization of volatile and semi-volatile compounds in green and fermented leaves of Bergenia crassifolia L. by gas chromatography-mass spectrometry and ID-CUBE direct analysis in real time-high resolution mass spectrometry.

    PubMed

    Chernetsova, Elena S; Shikov, Alexander N; Crawford, Elizabeth A; Grashorn, Sebastian; Laakso, I; Pozharitskaya, Olga N; Makarov, Valery G; Hiltunen, Raimo; Galambosi, Bertalan; Morlock, Gertrud E

    2014-01-01

    Chemical compositions of volatile and semi-volatile components in green and fermented leaves of Bergenia crassifolia L. were studied. Leaf components were identified using gas chromatography with low resolution mass spectrometry and direct analysis in real time (DART) high resolution mass spectrometry with an ID-CUBE ion source. Phytol, nerolidol, geraniol, linalool, alpha-bisabolol, alpha-bisabololoxide B, alpha-cadinol, delta-cadinene, alpha-terpineol and several other marker compounds of special interest were defined, for which the process of fermentation significantly changed their content in the leaves. Low resolution El GC-MS and ID-CUBE DART-HRMS were found to be complementary methods, as they provide different information, helpful to increase the confidence of identification.

  5. Interactive real time flow simulations

    NASA Technical Reports Server (NTRS)

    Sadrehaghighi, I.; Tiwari, S. N.

    1990-01-01

    An interactive real time flow simulation technique is developed for an unsteady channel flow. A finite-volume algorithm in conjunction with a Runge-Kutta time stepping scheme was developed for two-dimensional Euler equations. A global time step was used to accelerate convergence of steady-state calculations. A raster image generation routine was developed for high speed image transmission which allows the user to have direct interaction with the solution development. In addition to theory and results, the hardware and software requirements are discussed.

  6. Radiation damping in real time.

    PubMed

    Mendes, A C; Takakura, F I

    2001-11-01

    We study the nonequilibrium dynamics of a charge interacting with its own radiation, which originates the radiation damping. The real-time equation of motion for the charge and the associated Langevin equation is found in classical limit. The equation of motion for the charge allows one to obtain the frequency-dependent coefficient of friction. In the lowest order we find that although the coefficient of static friction vanishes, there is dynamical dissipation represented by a non-Markovian dissipative kernel.

  7. Real-time flutter analysis

    NASA Technical Reports Server (NTRS)

    Walker, R.; Gupta, N.

    1984-01-01

    The important algorithm issues necessary to achieve a real time flutter monitoring system; namely, the guidelines for choosing appropriate model forms, reduction of the parameter convergence transient, handling multiple modes, the effect of over parameterization, and estimate accuracy predictions, both online and for experiment design are addressed. An approach for efficiently computing continuous-time flutter parameter Cramer-Rao estimate error bounds were developed. This enables a convincing comparison of theoretical and simulation results, as well as offline studies in preparation for a flight test. Theoretical predictions, simulation and flight test results from the NASA Drones for Aerodynamic and Structural Test (DAST) Program are compared.

  8. Rapid identification of additives in poly(vinyl chloride) lid gaskets by direct analysis in real time ionisation and single-quadrupole mass spectrometry.

    PubMed

    Rothenbacher, Thorsten; Schwack, Wolfgang

    2010-01-01

    Gaskets for lids of glass jars usually consist of poly(vinyl chloride) (PVC) containing plasticisers and additional additives, which may migrate into packed foodstuffs. To conform to legal regulations, any such migration has to be determined analytically, which is a big challenge due to the huge chemical variety of additives in use. Therefore, a rapid screening method by means of direct analysis in real time mass spectrometry (DART-MS), using a single-quadrupole mass spectrometer, was developed. On introducing a plastisol sample into the DART interface, protonated molecules and ammonium adducts were obtained as the typical ionisation products of any additives present, and cleavages of ester bonds as typical fragmentation processes. Generally, additives present in the 1% range could be directly and easily identified if ion suppressive effects deriving from specific molecules did not occur. These effects could be avoided by analysing toluene extracts of plastisol samples, and this also improved the sensitivity. Using this method, it was possible to identify phthalates, fatty acid amides, tributyl O-acetylcitrate, dibutyl sebacate, bis(2-ethylhexyl) adipate, 1,2-diisononyl 1,2-cyclohexanedicarboxylate, and even more complex additives like acetylated mono- and diacylglycerides, epoxidised soybean oil, and polyadipates, with a limit of detection of < or = 1% in PVC plastisols. Only in the case of epoxidised linseed oil were levels of > or = 5% required for identification. The detection of azodicarbonamide, used as a foaming agent within the manufacturing process, was possible in principle, but was not highly reproducible due to the very low concentrations in plastisols.

  9. Proton-transfer-reaction mass spectrometry as a new tool for real time analysis of root-secreted volatile organic compounds in Arabidopsis.

    PubMed

    Steeghs, Marco; Bais, Harsh Pal; de Gouw, Joost; Goldan, Paul; Kuster, William; Northway, Megan; Fall, Ray; Vivanco, Jorge M

    2004-05-01

    Plant roots release about 5% to 20% of all photosynthetically-fixed carbon, and as a result create a carbon-rich environment for numerous rhizosphere organisms, including plant pathogens and symbiotic microbes. Although some characterization of root exudates has been achieved, especially of secondary metabolites and proteins, much less is known about volatile organic compounds (VOCs) released by roots. In this communication, we describe a novel approach to exploring these rhizosphere VOCs and their induction by biotic stresses. The VOC formation of Arabidopsis roots was analyzed using proton-transfer-reaction mass spectrometry (PTR-MS), a new technology that allows rapid and real time analysis of most biogenic VOCs without preconcentration or chromatography. Our studies revealed that the major VOCs released and identified by both PTR-MS and gas chromatography-mass spectrometry were either simple metabolites, ethanol, acetaldehyde, acetic acid, ethyl acetate, 2-butanone, 2,3,-butanedione, and acetone, or the monoterpene, 1,8-cineole. Some VOCs were found to be produced constitutively regardless of the treatment; other VOCs were induced specifically as a result of different compatible and noncompatible interactions between microbes and insects and Arabidopsis roots. Compatible interactions of Pseudomonas syringae DC3000 and Diuraphis noxia with Arabidopsis roots resulted in the rapid release of 1,8-cineole, a monoterpene that has not been previously reported in Arabidopsis. Mechanical injuries to Arabidopsis roots did not produce 1,8-cineole nor any C6 wound-VOCs; compatible interactions between Arabidopsis roots and Diuraphis noxia did not produce any wound compounds. This suggests that Arabidopsis roots respond to wounding differently from above-ground plant organs. Trials with incompatible interactions did not reveal a set of compounds that was significantly different compared to the noninfected roots. The PTR-MS method may open the way for functional root VOC

  10. Proton-Transfer-Reaction Mass Spectrometry as a New Tool for Real Time Analysis of Root-Secreted Volatile Organic Compounds in Arabidopsis1

    PubMed Central

    Steeghs, Marco; Bais, Harsh Pal; de Gouw, Joost; Goldan, Paul; Kuster, William; Northway, Megan; Fall, Ray; Vivanco, Jorge M.

    2004-01-01

    Plant roots release about 5% to 20% of all photosynthetically-fixed carbon, and as a result create a carbon-rich environment for numerous rhizosphere organisms, including plant pathogens and symbiotic microbes. Although some characterization of root exudates has been achieved, especially of secondary metabolites and proteins, much less is known about volatile organic compounds (VOCs) released by roots. In this communication, we describe a novel approach to exploring these rhizosphere VOCs and their induction by biotic stresses. The VOC formation of Arabidopsis roots was analyzed using proton-transfer-reaction mass spectrometry (PTR-MS), a new technology that allows rapid and real time analysis of most biogenic VOCs without preconcentration or chromatography. Our studies revealed that the major VOCs released and identified by both PTR-MS and gas chromatography-mass spectrometry were either simple metabolites, ethanol, acetaldehyde, acetic acid, ethyl acetate, 2-butanone, 2,3,-butanedione, and acetone, or the monoterpene, 1,8-cineole. Some VOCs were found to be produced constitutively regardless of the treatment; other VOCs were induced specifically as a result of different compatible and noncompatible interactions between microbes and insects and Arabidopsis roots. Compatible interactions of Pseudomonas syringae DC3000 and Diuraphis noxia with Arabidopsis roots resulted in the rapid release of 1,8-cineole, a monoterpene that has not been previously reported in Arabidopsis. Mechanical injuries to Arabidopsis roots did not produce 1,8-cineole nor any C6 wound-VOCs; compatible interactions between Arabidopsis roots and Diuraphis noxia did not produce any wound compounds. This suggests that Arabidopsis roots respond to wounding differently from above-ground plant organs. Trials with incompatible interactions did not reveal a set of compounds that was significantly different compared to the noninfected roots. The PTR-MS method may open the way for functional root VOC

  11. Assessing direct analysis in real-time-mass spectrometry (DART-MS) for the rapid identification of additives in food packaging.

    PubMed

    Ackerman, L K; Noonan, G O; Begley, T H

    2009-12-01

    The ambient ionization technique direct analysis in real time (DART) was characterized and evaluated for the screening of food packaging for the presence of packaging additives using a benchtop mass spectrometer (MS). Approximate optimum conditions were determined for 13 common food-packaging additives, including plasticizers, anti-oxidants, colorants, grease-proofers, and ultraviolet light stabilizers. Method sensitivity and linearity were evaluated using solutions and characterized polymer samples. Additionally, the response of a model additive (di-ethyl-hexyl-phthalate) was examined across a range of sample positions, DART, and MS conditions (temperature, voltage and helium flow). Under optimal conditions, molecular ion (M+H+) was the major ion for most additives. Additive responses were highly sensitive to sample and DART source orientation, as well as to DART flow rates, temperatures, and MS inlet voltages, respectively. DART-MS response was neither consistently linear nor quantitative in this setting, and sensitivity varied by additive. All additives studied were rapidly identified in multiple food-packaging materials by DART-MS/MS, suggesting this technique can be used to screen food packaging rapidly. However, method sensitivity and quantitation requires further study and improvement.

  12. Rapid screening and quantification of residual pesticides and illegal adulterants in red wine by direct analysis in real time mass spectrometry.

    PubMed

    Guo, Tianyang; Fang, Pingping; Jiang, Juanjuan; Zhang, Feng; Yong, Wei; Liu, Jiahui; Dong, Yiyang

    2016-11-04

    A rapid method to screen and quantify multi-class analytic targets in red wine has been developed by direct analysis in real time (DART) coupled with triple quadruple tandem mass spectrometry (QqQ-MS). A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) procedure was used for increasing analytical speed and reducing matrix effect, and the multiple reaction monitoring (MRM) in DART-MS/MS ensured accurate analysis. One bottle of wine containing 50 pesticides and 12 adulterants, i.e., preservatives, antioxidant, sweeteners, and azo dyes, could be totally determined less than 12min. This method exhibited proper linearity (R(2)≥0.99) in the range of 1-1000ng/mL for pesticides and 10-5000ng/mL for adulterants. The limits of detection (LODs) were obtained in a 0.5-50ng/mL range for pesticides and 5-50ng/mL range for adulterants, and the limits of quantification (LOQs) were in a 1-100ng/mL range for pesticides and 10-250ng/mL range for adulterants. Three spiked levels for each analyte in wine were evaluated, and the recoveries were in a scope of 75-120%. The results demonstrated DART-MS/MS was a rapid and simple method, and could be applied to rapid analyze residual pesticides and illegal adulterants in a large quantities of red wine.

  13. Using non-targeted direct analysis in real time-mass spectrometry (DART-MS) to discriminate seeds based on endogenous or exogenous chemicals.

    PubMed

    Subbaraj, Arvind K; Barrett, Brent A; Wakelin, Steve A; Fraser, Karl

    2015-10-01

    Forage seeds are a highly traded agricultural commodity, and therefore, quality control and assurance is high priority. In this study, we have used direct analysis in real time-mass spectrometry (DART-MS) as a tool to discriminate forage seeds based on their non-targeted chemical profiles. In the first experiment, two lots of perennial ryegrass (Lolium perenne L.) seed were discriminated based on exogenous residues of N-(3, 4-dichlorophenyl)-N,N-dimethylurea (Diuron(TM)), a herbicide. In a separate experiment, washed and unwashed seeds of the forage legumes white clover (Trifolium repens L.) and alfalfa (Medicago sativa L.) were discriminated based on the presence or absence of oxylipins, a class of endogenous antimicrobial compounds. Unwashed seeds confer toxicity towards symbiotic, nitrogen-fixing rhizobia which are routinely coated on legume seeds before planting, resulting in reduced rhizobial count. This is the first report of automatic introduction of intact seeds in the DART ion source and detecting oxylipins using DART-MS. Apart from providing scope to investigate legume-rhizobia symbiosis further in the context of oxylipins, the results presented here will enable future studies aimed at classification of seeds based on chemicals bound to the seed coat, thereby offering an efficient screening device for industry.

  14. Direct analysis in real time mass spectrometry combined with single-drop liquid-liquid-liquid microextraction for the rapid analysis of multiple phytohormones in fruit juice.

    PubMed

    Bai, Yu; Zhang, Jialing; Bai, Yu; Liu, Huwei

    2012-06-01

    A rapid, simple, and efficient method for the fast determination of multiple phytohormones was developed in this work, based on single-drop liquid-liquid-liquid microextraction (SD-LLLME) combined with direct analysis in real-time mass spectrometry (DART-MS). Six phytohormones--indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA), and gibberellin A(3) (GA(3))--were analyzed simultaneously using this method, and the conditions employed for DART-MS and SD-LLLME were optimized systematically. Satisfactory results were obtained in terms of linearity (R (2) values for all phytohormones were 0.991-0.996), sensitivity (limits of detection were 0.65-72 ng/mL), and repeatability (RSD values were 6.9-14%). In addition, the proposed method was applied to determine the endogenous phytohormones in three kinds of fruit juice. Different concentrations of phytohormones were detected with satisfactory recoveries, and the whole analytical procedure took no more than 30 min. Therefore, this combination of SD-LLLME and DART-MS was shown to be a suitable and effective approach for the fast analysis of targets present at trace level concentrations in complex matrices.

  15. On-line coupling of macroporous resin column chromatography with direct analysis in real time mass spectrometry utilizing a surface flowing mode sample holder.

    PubMed

    Zeng, Shanshan; Wang, Lu; Chen, Teng; Qu, Haibin

    2014-02-06

    A surface flowing mode sample holder was designed as an alternative sampling strategy for direct analysis in real time mass spectrometry (DART-MS). With the sample holder, the on-line coupling of macroporous resin column chromatography with DART-MS was explored and the new system was employed to monitor the column chromatography elution process of Panax notoginseng. The effluent from macroporous resin column was first diluted and mixed with a derivatization reagent on-line, and the mixture was then directly transferred into the ionization region of DART-MS by the sample holder. Notoginsenosides were methylated and ionized in a metastable helium gas stream, and was introduced into MS for detection. The on-line system showed reasonable repeatability with a relative standard deviation of 12.3% for the peak area. Three notoginsenosides, i.e. notoginsenoside R1, ginsenoside Rb1 and ginsenoside Rg1, were simultaneously determined during the eluting process. The alteration of the chemical composition in the effluent was accurately identified in 9 min, agreeing well with the off-line analysis. The presented technique is more convenient compared to the traditional UPLC method. These results suggest that the surface flowing mode DART-MS has a good potential for the on-line process monitoring in the pharmaceutical industry.

  16. A method for rapid sampling and characterization of smokeless powder using sorbent-coated wire mesh and direct analysis in real time - mass spectrometry (DART-MS).

    PubMed

    Li, Frederick; Tice, Joseph; Musselman, Brian D; Hall, Adam B

    2016-09-01

    Improvised explosive devices (IEDs) are often used by terrorists and criminals to create public panic and destruction, necessitating rapid investigative information. However, backlogs in many forensic laboratories resulting in part from time-consuming GC-MS and LC-MS techniques prevent prompt analytical information. Direct analysis in real time - mass spectrometry (DART-MS) is a promising analytical technique that can address this challenge in the forensic science community by permitting rapid trace analysis of energetic materials. Therefore, we have designed a qualitative analytical approach that utilizes novel sorbent-coated wire mesh and dynamic headspace concentration to permit the generation of information rich chemical attribute signatures (CAS) for trace energetic materials in smokeless powder with DART-MS. Sorbent-coated wire mesh improves the overall efficiency of capturing trace energetic materials in comparison to swabbing or vacuuming. Hodgdon Lil' Gun smokeless powder was used to optimize the dynamic headspace parameters. This method was compared to traditional GC-MS methods and validated using the NIST RM 8107 smokeless powder reference standard. Additives and energetic materials, notably nitroglycerin, were rapidly and efficiently captured by the Carbopack X wire mesh, followed by detection and identification using DART-MS. This approach has demonstrated the capability of generating comparable results with significantly reduced analysis time in comparison to GC-MS. All targeted components that can be detected by GC-MS were detected by DART-MS in less than a minute. Furthermore, DART-MS offers the advantage of detecting targeted analytes that are not amenable to GC-MS. The speed and efficiency associated with both the sample collection technique and DART-MS demonstrate an attractive and viable potential alternative to conventional techniques.

  17. Real-time pulmonary graphics.

    PubMed

    Mammel, Mark C; Donn, Steven M

    2015-06-01

    Real-time pulmonary graphics now enable clinicians to view lung mechanics and patient-ventilator interactions on a breath-to-breath basis. Displays of pressure, volume, and flow waveforms, pressure-volume and flow-volume loops, and trend screens enable clinicians to customize ventilator settings based on the underlying pathophysiology and responses of the individual patient. This article reviews the basic concepts of pulmonary graphics and demonstrates how they contribute to our understanding of respiratory physiology and the management of neonatal respiratory failure.

  18. Real-time streamflow conditions

    USGS Publications Warehouse

    Graczyk, David J.; Gebert, Warren A.

    1996-01-01

    Would you like to know streamflow conditions before you go fishing in Wisconsin or in more distant locations? Real-time streamflow data throughout Wisconsin and the United States are available on the Internet from the U.S. Geological Survey. You can see if the stream you are interested in fishing is high due to recent rain or low because of an extended dry spell. Flow conditions at more than 100 stream-gaging stations located throughout Wisconsin can be viewed by accessing the Wisconsin District Home Page at: http://wwwdwimdn.er.usgs.gov

  19. Real time infrared aerosol analyzer

    DOEpatents

    Johnson, Stanley A.; Reedy, Gerald T.; Kumar, Romesh

    1990-01-01

    Apparatus for analyzing aerosols in essentially real time includes a virtual impactor which separates coarse particles from fine and ultrafine particles in an aerosol sample. The coarse and ultrafine particles are captured in PTFE filters, and the fine particles impact onto an internal light reflection element. The composition and quantity of the particles on the PTFE filter and on the internal reflection element are measured by alternately passing infrared light through the filter and the internal light reflection element, and analyzing the light through infrared spectrophotometry to identify the particles in the sample.

  20. Real-time analysis keratometer

    NASA Technical Reports Server (NTRS)

    Adachi, Iwao P. (Inventor); Adachi, Yoshifumi (Inventor); Frazer, Robert E. (Inventor)

    1987-01-01

    A computer assisted keratometer in which a fiducial line pattern reticle illuminated by CW or pulsed laser light is projected on a corneal surface through lenses, a prismoidal beamsplitter quarterwave plate, and objective optics. The reticle surface is curved as a conjugate of an ideal corneal curvature. The fiducial image reflected from the cornea undergoes a polarization shift through the quarterwave plate and beamsplitter whereby the projected and reflected beams are separated and directed orthogonally. The reflected beam fiducial pattern forms a moire pattern with a replica of the first recticle. This moire pattern contains transverse aberration due to differences in curvature between the cornea and the ideal corneal curvature. The moire pattern is analyzed in real time by computer which displays either the CW moire pattern or a pulsed mode analysis of the transverse aberration of the cornea under observation, in real time. With the eye focused on a plurality of fixation points in succession, a survey of the entire corneal topography is made and a contour map or three dimensional plot of the cornea can be made as a computer readout in addition to corneal radius and refractive power analysis.

  1. Real-time analysis of ambient organic aerosols using aerosol flowing atmospheric-pressure afterglow mass spectrometry (AeroFAPA-MS)

    NASA Astrophysics Data System (ADS)

    Brüggemann, Martin; Karu, Einar; Stelzer, Torsten; Hoffmann, Thorsten

    2015-04-01

    Organic aerosol accounts for a major fraction of atmospheric aerosols and has implications on the earth's climate and human health. However, due to the chemical complexity its measurement remains a major challenge for analytical instrumentation.1 Here, we present the development, characterization and application of a new soft ionization technique that allows mass spectrometric real-time detection of organic compounds in ambient aerosols. The aerosol flowing atmospheric-pressure afterglow (AeroFAPA) ion source utilizes a helium glow discharge plasma to produce excited helium species and primary reagent ions. Ionization of the analytes occurs in the afterglow region after thermal desorption and results mainly in intact molecular ions, facilitating the interpretation of the acquired mass spectra. In the past, similar approaches were used to detect pesticides, explosives or illicit drugs on a variety of surfaces.2,3 In contrast, the AeroFAPA source operates 'online' and allows the detection of organic compounds in aerosols without a prior precipitation or sampling step. To our knowledge, this is the first application of an atmospheric-pressure glow discharge ionization technique to ambient aerosol samples. We illustrate that changes in aerosol composition and concentration are detected on the time scale of seconds and in the ng-m-3 range. Additionally, the successful application of AeroFAPA-MS during a field study in a mixed forest region in Central Europe is presented. Several oxidation products of monoterpenes were clearly identified using the possibility to perform tandem MS experiments. The acquired data are in agreement with previous studies and demonstrate that AeroFAPA-MS is a suitable tool for organic aerosol analysis. Furthermore, these results reveal the potential of this technique to enable new insights into aerosol formation, growth and transformation in the atmosphere. References: 1) IPCC, 2013: Summary for Policymakers. In: Climate Change 2013: The

  2. Real-time flood forecasting

    USGS Publications Warehouse

    Lai, C.; Tsay, T.-K.; Chien, C.-H.; Wu, I.-L.

    2009-01-01

    Researchers at the Hydroinformatic Research and Development Team (HIRDT) of the National Taiwan University undertook a project to create a real time flood forecasting model, with an aim to predict the current in the Tamsui River Basin. The model was designed based on deterministic approach with mathematic modeling of complex phenomenon, and specific parameter values operated to produce a discrete result. The project also devised a rainfall-stage model that relates the rate of rainfall upland directly to the change of the state of river, and is further related to another typhoon-rainfall model. The geographic information system (GIS) data, based on precise contour model of the terrain, estimate the regions that were perilous to flooding. The HIRDT, in response to the project's progress, also devoted their application of a deterministic model to unsteady flow of thermodynamics to help predict river authorities issue timely warnings and take other emergency measures.

  3. Office Chromatography: Precise printing of sample solutions on miniaturized thin-layer phases and utilization for scanning Direct Analysis in Real Time mass spectrometry.

    PubMed

    Häbe, Tim T; Morlock, Gertrud E

    2015-09-25

    Office Chromatography combines achievements in office technologies with miniaturized planar chromatography. In the life sciences, printing of materials became an accepted technique, whereas in separation science, the use of printers for chromatography is at its infancy. A bubble-jet printer was modified for exact application on miniaturized plates. Technical modifications included the removal of all unnecessary parts and the improvement of the positioning system, purge unit and sample supply system. Evaluation was performed via a slide scanner and image evaluation software. Printing of a food dye mixture solution (n=5) led to a calculated mean deposition volume of 13±1nL/mm(2) per print-cycle. A mean determination coefficient (R(2); n=5) of 0.9990 was obtained for application of increasing volumes, executed via increasing band widths of 50-200μm (corresponding to 2-8nL). Using larger band widths and multiple print jobs, deposition volumes of up to the microliter scale represented an alternative to cost-intensive standard equipment. After print, separation, detection and digital evaluation of five food dyes, mean R(2) (n=5) were obtained between 0.9977 and 0.9995. The accuracy of printing was proven by mean recovery rates of 101-105% with repeatabilities of 3-7% (%RSD, n=5). The transfer to nanostructured ultrathin-layer plates proved the synergetic potential of these fields of research. First, this modified printer was suited for printing of finely graduated scales of three preservatives for determination of the spatial resolution of scanning Direct Analysis in Real Time mass spectrometry.

  4. Determination of the aflatoxin M1 (AFM1) from milk by direct analysis in real time - mass spectrometry (DART-MS)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Certain fungi that grow on crops can produce aflatoxins, which are highly carcinogenic. One of these, aflatoxin B1 can be metabolized by mammals to aflatoxin M1, a form that retains potent carcinogenicity and which can be excreted into milk. Direct analysis in real time (DART) ionization coupled to ...

  5. Autonomous Real Time Requirements Tracing

    NASA Technical Reports Server (NTRS)

    Plattsmier, George I.; Stetson, Howard K.

    2014-01-01

    One of the more challenging aspects of software development is the ability to verify and validate the functional software requirements dictated by the Software Requirements Specification (SRS) and the Software Detail Design (SDD). Insuring the software has achieved the intended requirements is the responsibility of the Software Quality team and the Software Test team. The utilization of Timeliner-TLX(sup TM) Auto-Procedures for relocating ground operations positions to ISS automated on-board operations has begun the transition that would be required for manned deep space missions with minimal crew requirements. This transition also moves the auto-procedures from the procedure realm into the flight software arena and as such the operational requirements and testing will be more structured and rigorous. The autoprocedures would be required to meet NASA software standards as specified in the Software Safety Standard (NASASTD- 8719), the Software Engineering Requirements (NPR 7150), the Software Assurance Standard (NASA-STD-8739) and also the Human Rating Requirements (NPR-8705). The Autonomous Fluid Transfer System (AFTS) test-bed utilizes the Timeliner-TLX(sup TM) Language for development of autonomous command and control software. The Timeliner- TLX(sup TM) system has the unique feature of providing the current line of the statement in execution during real-time execution of the software. The feature of execution line number internal reporting unlocks the capability of monitoring the execution autonomously by use of a companion Timeliner-TLX(sup TM) sequence as the line number reporting is embedded inside the Timeliner-TLX(sup TM) execution engine. This negates I/O processing of this type data as the line number status of executing sequences is built-in as a function reference. This paper will outline the design and capabilities of the AFTS Autonomous Requirements Tracker, which traces and logs SRS requirements as they are being met during real-time execution of the

  6. Autonomous Real Time Requirements Tracing

    NASA Technical Reports Server (NTRS)

    Plattsmier, George; Stetson, Howard

    2014-01-01

    One of the more challenging aspects of software development is the ability to verify and validate the functional software requirements dictated by the Software Requirements Specification (SRS) and the Software Detail Design (SDD). Insuring the software has achieved the intended requirements is the responsibility of the Software Quality team and the Software Test team. The utilization of Timeliner-TLX(sup TM) Auto- Procedures for relocating ground operations positions to ISS automated on-board operations has begun the transition that would be required for manned deep space missions with minimal crew requirements. This transition also moves the auto-procedures from the procedure realm into the flight software arena and as such the operational requirements and testing will be more structured and rigorous. The autoprocedures would be required to meet NASA software standards as specified in the Software Safety Standard (NASASTD- 8719), the Software Engineering Requirements (NPR 7150), the Software Assurance Standard (NASA-STD-8739) and also the Human Rating Requirements (NPR-8705). The Autonomous Fluid Transfer System (AFTS) test-bed utilizes the Timeliner-TLX(sup TM) Language for development of autonomous command and control software. The Timeliner-TLX(sup TM) system has the unique feature of providing the current line of the statement in execution during real-time execution of the software. The feature of execution line number internal reporting unlocks the capability of monitoring the execution autonomously by use of a companion Timeliner-TLX(sup TM) sequence as the line number reporting is embedded inside the Timeliner-TLX(sup TM) execution engine. This negates I/O processing of this type data as the line number status of executing sequences is built-in as a function reference. This paper will outline the design and capabilities of the AFTS Autonomous Requirements Tracker, which traces and logs SRS requirements as they are being met during real-time execution of the

  7. Real-time optical tweezing

    NASA Astrophysics Data System (ADS)

    Rahman, Shah Mohammed Tamzidur

    In this thesis a new approach called ‘space-time-wavelength mapping’ has been developed for real-time electronic control of optical tweezers. The proposed technique enables precise control of optical signals in space, time, and frequency through time-domain dispersion and diffractive optics, which in turn enables generation of controlled radiation forces acting on small particles. In this study we show that 150 fs ultrafast optical pulses can be dispersed in time and space to achieve a 20 μm x 2 μm focused elliptical beam. The force field at the focal plane of the beam is dependent on local intensity gradients along the plane. The spatial intensity profile can be electronically controlled by assigning local power levels to each wavelength using time-domain RF modulation of dispersed pulses, and sending each wavelength, and hence the assigned power level, to a specific location in space through diffractive optics. We show that by choosing the appropriate RF waveform, one is able to create force fields for cell stretching and compression as well as multiple force hot-spots (of >200 pN force per pulse) for attractive and repulsive forces. A detailed theoretical model and simulation results from a proposed experimental setup are presented. This approach is significantly more advantageous in terms of flexibility and control, compared to conventional optical tweezers that require mechanical steering or holographic optical tweezers that produce undesired ‘ghost traps’. In addition, it is shown how the technique can also be extended to create tunable 2D force field distributions using a virtually-imaged phased-array (VIPA).

  8. Clinical virology in real time.

    PubMed

    Niesters, Hubert G M

    2002-12-01

    The ability to detect nucleic acids has had and still has a major impact on diagnostics in clinical virology. Both quantitative and qualitative techniques, whether signal or target amplification based systems, are currently used routinely in most if not all virology laboratories. Technological improvements, from automated sample isolation to real time amplification technology, have given the ability to develop and introduce systems for most viruses of clinical interest, and to obtain clinical relevant information needed for optimal antiviral treatment options. Both polymerase chain reaction (PCR) and nucleic acid sequence-based amplification (NASBA) can currently be used together with real time detection to generate results in a short turn-around time and to determine whether variants relevant for antiviral resistance are present. These new technologies enable the introduction of an individual patient disease management concept. Within our clinical setting, we have introduced this e.g. for quantitative detection of Epstein-Barr Virus (EBV) in T-dell depleted allogeneic stem cell transplant patients. This enabled us to develop models for pre-emptive anti B-cell immunotherapy for EBV reactivation, thereby effectively reducing not the incidence of EBV-lymphoproliferative disease but the virus related mortality. Furthermore, additional clinically relevant viruses can now easily be detected simultaneously. It also becomes more feasible to introduce molecular testing for those viruses that can easily be detected using classical virological methods, like culture techniques or antigen detection. Prospective studies are needed to evaluate the clinical importance of the additional positive samples detected. It should however be made clear that a complete exchange of technologies is unlikely to occur, and that some complementary technologies should stay operational enabling the discovery of new viruses. The implementation of these molecular diagnostic technologies furthermore

  9. Rapid screening of 35 new psychoactive substances by ion mobility spectrometry (IMS) and direct analysis in real time (DART) coupled to quadrupole time-of-flight mass spectrometry (QTOF-MS).

    PubMed

    Gwak, Seongshin; Almirall, Jose R

    2015-10-01

    The recent propagation of new psychoactive substances (NPS) has led to the development of new techniques for the rapid characterization of controlled substances in this category. A commercial bench-top ion mobility spectrometer (IMS) with a (63) Ni ionization source and a direct analysis in real time (DART) coupled to quadrupole time-of-flight (QTOF) were used for the rapid characterization of 35 NPS. The advantages of these techniques are fast response, ease of operation, and minimal sample preparation. The characteristic reduced mobilities of each substance are reported as are the mass spectra of the 35 compounds. The acquired product ion scan mass spectra were also compared to a library database constructed by QTOF with a electrospray ionization (ESI) source and showed a consistent relative abundance for each peak over time. A total of four seized drug samples provided by the local forensic laboratory were analyzed in order to demonstrate the utility of this approach. The results of this study suggest that both IMS and DART-QTOF are promising alternatives for the rapid screening and characterization of these new psychoactive substances.

  10. Holin triggering in real time.

    PubMed

    White, Rebecca; Chiba, Shinobu; Pang, Ting; Dewey, Jill S; Savva, Christos G; Holzenburg, Andreas; Pogliano, Kit; Young, Ry

    2011-01-11

    During λ infections, the holin S105 accumulates harmlessly in the membrane until, at an allele-specific time, suddenly triggering to form irregular holes of unprecedented size (>300 nm), releasing the endolysin from the cytoplasm, resulting in lysis within seconds. Here we used a functional S105-GFP chimera and real-time deconvolution fluorescence microscopy to show that the S105-GFP fusion accumulated in a uniformly distributed fashion, until suddenly, within 1 min, it formed aggregates, or rafts, at the time of lethal triggering. Moreover, the isogenic fusion to a nonlethal S105 mutant remained uniformly distributed, whereas a fusion to an early-lysing mutant showed early triggering and early raft formation. Protein accumulation rates of the WT, early, and nonlethal alleles were identical. Fluorescence recovery after photobleaching (FRAP) revealed that the nonlethal mutant and untriggered WT hybrids were highly mobile in the membrane, whereas the WT raft was essentially immobile. Finally, an antiholin allele, S105(ΔTMD1)-mcherryfp, in the product of which the S105 sequence deleted for the first transmembrane domain was fused to mCherryFP. This hybrid retained full antiholin activity, in that it blocked lethal hole formation by the S105-GFP fusion, accumulated uniformly throughout the host membrane and prevented the S105-GFP protein from forming rafts. These findings suggest that phage lysis occurs when the holin reaches a critical concentration and nucleates to form rafts, analogous to the initiation of purple membrane formation after the induction of bacteriorhodopsin in halobacteria. This model for holin function may be relevant for processes in mammalian cells, including the release of nonenveloped viruses and apoptosis.

  11. Real-Time Data Display

    NASA Technical Reports Server (NTRS)

    Pedings, Marc

    2007-01-01

    RT-Display is a MATLAB-based data acquisition environment designed to use a variety of commercial off-the-shelf (COTS) hardware to digitize analog signals to a standard data format usable by other post-acquisition data analysis tools. This software presents the acquired data in real time using a variety of signal-processing algorithms. The acquired data is stored in a standard Operator Interactive Signal Processing Software (OISPS) data-formatted file. RT-Display is primarily configured to use the Agilent VXI (or equivalent) data acquisition boards used in such systems as MIDDAS (Multi-channel Integrated Dynamic Data Acquisition System). The software is generalized and deployable in almost any testing environment, without limitations or proprietary configuration for a specific test program or project. With the Agilent hardware configured and in place, users can start the program and, in one step, immediately begin digitizing multiple channels of data. Once the acquisition is completed, data is converted into a common binary format that also can be translated to specific formats used by external analysis software, such as OISPS and PC-Signal (product of AI Signal Research Inc.). RT-Display at the time of this reporting was certified on Agilent hardware capable of acquisition up to 196,608 samples per second. Data signals are presented to the user on-screen simultaneously for 16 channels. Each channel can be viewed individually, with a maximum capability of 160 signal channels (depending on hardware configuration). Current signal presentations include: time data, fast Fourier transforms (FFT), and power spectral density plots (PSD). Additional processing algorithms can be easily incorporated into this environment.

  12. Combining two-dimensional diffusion-ordered nuclear magnetic resonance spectroscopy, imaging desorption electrospray ionization mass spectrometry, and direct analysis in real-time mass spectrometry for the integral investigation of counterfeit pharmaceuticals.

    PubMed

    Nyadong, Leonard; Harris, Glenn A; Balayssac, Stéphane; Galhena, Asiri S; Malet-Martino, Myriam; Martino, Robert; Parry, R Mitchell; Wang, May Dongmei; Fernández, Facundo M; Gilard, Véronique

    2009-06-15

    During the past decade, there has been a marked increase in the number of reported cases involving counterfeit medicines in developing and developed countries. Particularly, artesunate-based antimalarial drugs have been targeted, because of their high demand and cost. Counterfeit antimalarials can cause death and can contribute to the growing problem of drug resistance, particularly in southeast Asia. In this study, the complementarity of two-dimensional diffusion-ordered (1)H nuclear magnetic resonance spectroscopy (2D DOSY (1)H NMR) with direct analysis in real-time mass spectrometry (DART MS) and desorption electrospray ionization mass spectrometry (DESI MS) was assessed for pharmaceutical forensic purposes. Fourteen different artesunate tablets, representative of what can be purchased from informal sources in southeast Asia, were investigated with these techniques. The expected active pharmaceutical ingredient was detected in only five formulations via both nuclear magnetic resonance (NMR) and mass spectrometry (MS) methods. Common organic excipients such as sucrose, lactose, stearate, dextrin, and starch were also detected. The graphical representation of DOSY (1)H NMR results proved very useful for establishing similarities among groups of samples, enabling counterfeit drug "chemotyping". In addition to bulk- and surface-average analyses, spatially resolved information on the surface composition of counterfeit and genuine antimalarial formulations was obtained using DESI MS that was performed in the imaging mode, which enabled one to visualize the homogeneity of both genuine and counterfeit drug samples. Overall, this study suggests that 2D DOSY (1)H NMR, combined with ambient MS, comprises a powerful suite of instrumental analysis methodologies for the integral characterization of counterfeit antimalarials.

  13. Real-Time Nonlinear Optical Information Processing.

    DTIC Science & Technology

    1979-06-01

    operations aree presented. One approach realizes the halftone method of nonlinear optical processing in real time by replacing the conventional...photographic recording medium with a real-time image transducer. In the second approach halftoning is eliminated and the real-time device is used directly

  14. Mobile real time radiography system

    SciTech Connect

    Vigil, J.; Taggart, D.; Betts, S.

    1997-11-01

    A 450-keV Mobile Real Time Radiography (RTR) System was delivered to Los Alamos National Laboratory (LANL) in January 1996. It was purchased to inspect containers of radioactive waste produced at (LANL). Since its delivery it has been used to radiograph more than 600 drums of radioactive waste at various LANL sites. It has the capability of inspecting waste containers of various sizes from <1-gal. buckets up to standard waste boxes (SWB, dimensions 54.5 in. x 71 in. x 37 in.). It has three independent x-ray acquisition formats. The primary system used is a 12- in. image intensifier, the second is a 36-in. linear diode array (LDA) and the last is an open system. It is fully self contained with on board generator, HVAC, and a fire suppression system. It is on a 53-ft long x 8-ft. wide x 14-ft. high trailer that can be moved over any highway requiring only an easily obtainable overweight permit because it weights {approximately}38 tons. It was built to conform to industry standards for a cabinet system which does not require an exclusion zone. The fact that this unit is mobile has allowed us to operate where the waste is stored, rather than having to move the waste to a fixed facility.

  15. Towards depth profiling of organic aerosols in real time using aerosol flowing atmospheric-pressure afterglow mass spectrometry (AeroFAPA-MS)

    NASA Astrophysics Data System (ADS)

    Brüggemann, Martin; Hoffmann, Thorsten

    2014-05-01

    Organic aerosol accounts for a substantial fraction of tropospheric aerosol and has implications on the earth's climate and human health. However, the characterization of its chemical composition and transformations remain a major challenge and is still connected to large uncertainties (IPCC, 2013). Recent measurements revealed that organic aerosol particles may reside in an amorphous or semi-solid phase state which impedes the diffusion within the particles (Virtanen et al., 2010; Shiraiwa et al., 2011). This means that reaction products which are formed on the surface of a particle, e.g. by OH, NO3 or ozone chemistry, cannot diffuse into the particle's core and remain at the surface. Eventually, this leads to particles with a core/shell structure. In the particles' cores the initial compounds are preserved whereas the shells contain mainly the oxidation products. By analyzing the particles' cores and shells separately, thus, it is possible to obtain valuable information on the formation and evolution of the aerosols' particle and gas phase. Here we present the development of the aerosol flowing atmospheric-pressure afterglow (AeroFAPA) technique which allows the mass spectrometric analysis of organic aerosols in real time. The AeroFAPA is an ion source based on a helium glow discharge at atmospheric pressure. The plasma produces excited helium species and primary reagent ions which are transferred into the afterglow region where the ionization of the analytes takes place. Due to temperatures of only 80 ° C to 150 ° C and ambient pressure in the afterglow region, the ionization is very soft and almost no fragmentation of organic molecules is observed. Thus, the obtained mass spectra are easy to interpret and no extensive data analysis procedure is necessary. Additionally, first results of a combination of the AeroFAPA-MS with a scanning mobility particle sizer (SMPS) suggest that it is not only possible to analyze the entire particle phase but rather that a

  16. Students Collecting Real time Data

    NASA Astrophysics Data System (ADS)

    Miller, P.

    2006-05-01

    Students Collecting Real-Time Data The Hawaiian Islands Humpback Whale National Marine Sanctuary has created opportunities for middle and high school students to become Student Researchers and to be involved in real-time marine data collection. It is important that we expose students to different fields of science and encourage them to enter scientific fields of study. The Humpback Whale Sanctuary has an education visitor center in Kihei, Maui. Located right on the beach, the site has become a living classroom facility. There is a traditional Hawaiian fishpond fronting the property. The fishpond wall is being restored, using traditional methods. The site has the incredible opportunity of incorporating Hawaiian cultural practices with scientific studies. The Sanctuary offers opportunities for students to get involved in monitoring and data collection studies. Invasive Seaweed Study: Students are collecting data on invasive seaweed for the University of Hawaii. They pull a large net through the shallow waters. Seaweed is sorted, identified and weighed. The invasive seaweeds are removed. The data is recorded and sent to UH. Remote controlled monitoring boats: The sanctuary has 6 boogie board sized remote controlled boats used to monitor reefs. Boats have a camera with lights on the underside. The boats have water quality monitoring devices and GPS units. The video from the underwater camera is transmitted via a wireless transmission. Students are able to monitor the fish, limu and invertebrate populations on the reef and collect water quality data via television monitors or computers. The boat can also pull a small plankton tow net. Data is being compiled into data bases. Artificial Reef Modules: The Sanctuary has a scientific permit from the state to build and deploy artificial reef modules. High school students are designing and building modules. These are deployed out in the Fishpond fronting the Sanctuary site and students are monitoring them on a weekly basis

  17. Real-time analysis of aromatics in combustion engine exhaust by resonance-enhanced multiphoton ionisation time-of-flight mass spectrometry (REMPI-TOF-MS): a robust tool for chassis dynamometer testing.

    PubMed

    Adam, T W; Clairotte, M; Streibel, T; Elsasser, M; Pommeres, A; Manfredi, U; Carriero, M; Martini, G; Sklorz, M; Krasenbrink, A; Astorga, C; Zimmermann, R

    2012-07-01

    Resonance-enhanced multiphoton ionisation time-of-flight mass spectrometry (REMPI-TOF-MS) is a robust method for real-time analysis of monocyclic and polycyclic aromatic hydrocarbons in complex emissions. A mobile system has been developed which enables direct analysis on site. In this paper, we utilize a multicomponent calibration scheme based on the analytes' photo-ionisation cross-sections relative to a calibrated species. This allows semi-quantification of a great number of components by only calibrating one compound of choice, here toluene. The cross-sections were determined by injecting nebulised solutions of aromatic compounds into the TOF-MS ion source with the help of a HPLC pump. Then, REMPI-TOF-MS was implemented at various chassis dynamometers and test cells and the exhaust of the following vehicles and engines investigated: a compression ignition light-duty (LD) passenger car, a compression ignition LD van, two spark ignition LD passenger cars, 2 two-stroke mopeds, and a two-stroke engine of a string gas trimmer. The quantitative time profiles of benzene are shown. The results indicate that two-stroke engines are a significant source for toxic and cancerogenic compounds. Air pollution and health effects caused by gardening equipment might still be underestimated.

  18. Research in Distributed Real-Time Systems

    NASA Technical Reports Server (NTRS)

    Mukkamala, R.

    1997-01-01

    This document summarizes the progress we have made on our study of issues concerning the schedulability of real-time systems. Our study has produced several results in the scalability issues of distributed real-time systems. In particular, we have used our techniques to resolve schedulability issues in distributed systems with end-to-end requirements. During the next year (1997-98), we propose to extend the current work to address the modeling and workload characterization issues in distributed real-time systems. In particular, we propose to investigate the effect of different workload models and component models on the design and the subsequent performance of distributed real-time systems.

  19. Real-time analysis of organic compounds in ship engine aerosol emissions using resonance-enhanced multiphoton ionisation and proton transfer mass spectrometry.

    PubMed

    Radischat, Christian; Sippula, Olli; Stengel, Benjamin; Klingbeil, Sophie; Sklorz, Martin; Rabe, Rom; Streibel, Thorsten; Harndorf, Horst; Zimmermann, Ralf

    2015-08-01

    Organic combustion aerosols from a marine medium-speed diesel engine, capable to run on distillate (diesel fuel) and residual fuels (heavy fuel oil), were investigated under various operating conditions and engine parameters. The online chemical characterisation of the organic components was conducted using a resonance-enhanced multiphoton ionisation time-of-flight mass spectrometer (REMPI TOF MS) and a proton transfer reaction-quadrupole mass spectrometer (PTR-QMS). Oxygenated species, alkenes and aromatic hydrocarbons were characterised. Especially the aromatic hydrocarbons and their alkylated derivatives were very prominent in the exhaust of both fuels. Emission factors of known health-hazardous compounds (e.g. mono- and poly-aromatic hydrocarbons) were calculated and found in higher amounts for heavy fuel oil (HFO) at typical engine loadings. Lower engine loads lead in general to increasing emissions for both fuels for almost every compound, e.g. naphthalene emissions varied for diesel fuel exhaust between 0.7 mg/kWh (75 % engine load, late start of injection (SOI)) and 11.8 mg/kWh (10 % engine load, late SOI) and for HFO exhaust between 3.3 and 60.5 mg/kWh, respectively. Both used mass spectrometric techniques showed that they are particularly suitable methods for online monitoring of combustion compounds and very helpful for the characterisation of health-relevant substances. Graphical abstract Three-dimensional REMPI data of organic species in diesel fuel and heavy fuel oil exhaust.

  20. Development of "Laser Ablation Direct Analysis in Real Time Imaging" Mass Spectrometry: Application to Spatial Distribution Mapping of Metabolites Along the Biosynthetic Cascade Leading to Synthesis of Atropine and Scopolamine in Plant Tissue.

    PubMed

    Fowble, Kristen L; Teramoto, Kanae; Cody, Robert B; Edwards, David; Guarrera, Donna; Musah, Rabi A

    2017-03-21

    Methods for the accomplishment of small-molecule imaging by mass spectrometry are challenged by the need for sample pretreatment steps, such as cryo-sectioning, dehydration, chemical fixation, or application of a matrix or solvent, that must be performed to obtain interpretable spatial distribution data. Furthermore, these steps along with requirements of the mass analyzer such as high vacuum, can severely limit the range of sample types that can be analyzed by this powerful method. Here, we report the development of a laser ablation-direct analysis in real time imaging mass spectrometry approach which couples a 213 nm Nd:YAG solid state UV laser to a direct analysis in a real time ion source and high-resolution time-of-flight mass spectrometer. This platform enables facile determination of the spatial distribution of small-molecules spanning a range of polarities in a diversity of sample types and requires no matrix, vacuum, solvent, or complicated sample pretreatment steps. It furnishes high-resolution data, can be performed under ambient conditions on samples in their native form, and results in little to no fragmentation of analytes. We demonstrate its application through determination of the spatial distribution of molecules involved in the biosynthetic cascade leading to formation of the clinically relevant alkaloids atropine and scopolamine in Datura leichhardtii seed tissue.

  1. In-line real time air monitor

    DOEpatents

    Wise, Marcus B.; Thompson, Cyril V.

    1998-01-01

    An in-line gas monitor capable of accurate gas composition analysis in a continuous real time manner even under strong applied vacuum conditions operates by mixing an air sample with helium forming a sample gas in two complementary sample loops embedded in a manifold which includes two pairs of 3-way solenoid valves. The sample gas is then analyzed in an ion trap mass spectrometer on a continuous basis. Two valve drivers actuate the two pairs of 3-way valves in a reciprocating fashion, so that there is always flow through the in-line gas monitor via one or the other of the sample loops. The duty cycle for the two pairs of 3-way valves is varied by tuning the two valve drivers to a duty cycle typically between 0.2 to 0.7 seconds.

  2. In-line real time air monitor

    DOEpatents

    Wise, M.B.; Thompson, C.V.

    1998-07-14

    An in-line gas monitor capable of accurate gas composition analysis in a continuous real time manner even under strong applied vacuum conditions operates by mixing an air sample with helium forming a sample gas in two complementary sample loops embedded in a manifold which includes two pairs of 3-way solenoid valves. The sample gas is then analyzed in an ion trap mass spectrometer on a continuous basis. Two valve drivers actuate the two pairs of 3-way valves in a reciprocating fashion, so that there is always flow through the in-line gas monitor via one or the other of the sample loops. The duty cycle for the two pairs of 3-way valves is varied by tuning the two valve drivers to a duty cycle typically between 0.2 to 0.7 seconds. 3 figs.

  3. Volatile Organic Compound emissions from soil: using Proton-Transfer-Reaction Time-of-Flight Mass Spectrometry (PTR-TOF-MS) for the real time observation of microbial processes

    NASA Astrophysics Data System (ADS)

    Veres, P. R.; Behrendt, T.; Klapthor, A.; Meixner, F. X.; Williams, J.

    2014-08-01

    In this study we report on the emissions of volatile organic compounds (VOC) and nitric oxide (NO) from two contrasting soils (equatorial rainforest and arid cotton field) analyzed in a laboratory based dynamic chamber system. The effect of soil moisture and soil temperature on VOC and NO emission was examined in laboratory incubation experiments by measuring as a pre-saturated soil dried out. Our results suggest that real time monitoring of VOC emissions from soil using a proton-transfer-reaction time-of-flight mass spectrometer (PTR-TOF-MS) instrument can be used to improve our understanding of the release mechanisms of trace gases (e.g. NO, N2O) that are involved in the nitrogen cycle. Moreover, we report on the release rate of various VOC species, many of which exhibit a temperature dependent response indicative of biological production, namely a temperature amplification factor (Q10) ∼ 2-3. Contrary to the conventional modeling of NO emissions from soils, that the release of NO from the overall community across the range of soil water content can be modeled as an optimum function, we suggest that VOC measurements indicate there exist multiple distinct contributing microbial guilds releasing NO. These microbial guilds could likely be individually identified with the observed VOC profiles. Using a cotton field soil sample from a Sache oasis (Taklimakan desert, Xinijang, P. R. China), we identify five VOC emission groups with varying degrees of NO co-emission. An equatorial rainforest soil (Suriname) was shown to emit a variety of VOC including acetaldehyde, acetone, DMS, formaldehyde, and isoprene that vary strongly and individually as a function of temperature and soil moisture content. PTR-TOF-MS with high time resolution, sensitivity, and molecular specificity is an ideal tool for the real time analysis of VOC and NO emitting processes in soil systems. These experiments can be used as a template for future experiments to more completely and specifically

  4. Real time programming environment for Windows

    SciTech Connect

    LaBelle, D.R.

    1998-04-01

    This document provides a description of the Real Time Programming Environment (RTProE). RTProE tools allow a programmer to create soft real time projects under general, multi-purpose operating systems. The basic features necessary for real time applications are provided by RTProE, leaving the programmer free to concentrate efforts on his specific project. The current version supports Microsoft Windows{trademark} 95 and NT. The tasks of real time synchronization and communication with other programs are handled by RTProE. RTProE includes a generic method for connecting a graphical user interface (GUI) to allow real time control and interaction with the programmer`s product. Topics covered in this paper include real time performance issues, portability, details of shared memory management, code scheduling, application control, Operating System specific concerns and the use of Computer Aided Software Engineering (CASE) tools. The development of RTProE is an important step in the expansion of the real time programming community. The financial costs associated with using the system are minimal. All source code for RTProE has been made publicly available. Any person with access to a personal computer, Windows 95 or NT, and C or FORTRAN compilers can quickly enter the world of real time modeling and simulation.

  5. [Real-time analysis of polyvinyl chloride thermal decomposition/combustion products with single photon ionization/photoelectron ionization online mass spectrometer].

    PubMed

    Chen, Wen-Dong; Hou, Ke-Yong; Chen, Ping; Li, Fang-Long; Zhao, Wu-Duo; Cui, Hua-Peng; Hua, Lei; Xie, Yuan-Yuan; Li, Hai-Yang

    2013-01-01

    With the features of a broad range of ionizable compounds, reduced fragments and simple mass spectrum, a homemade magnetic field enhanced photoelectron ionization (MEPEI) source combined with single photon ionization (SPI) for time-of-flight mass spectrometer was built and applied to analyze thermal decomposition/combustion products of polyvinyl chloride (PVC). The combined ion source can be switched very fast between SPI mode and SPI-MEPEI mode for detecting different targeted compounds, and only adjusting the voltage of the electrode in the ionization region to trigger the switch. Among the PVC thermal decomposition/combustion products, HCl and CO2, which ionization energies (12.74 eV, 13.77 eV respectively) were higher than the energy of photon (10.60 eV), were ionized by MEPEI, while alkenes, dichloroethylene, benzene and its homologs, monochlorobenzene, styrene, indane, naphthalene and its homologs were ionized by SPI and MEPEI simultaneously. Spectra of interested products as a function of temperatures indicated that products are formed via two main mechanisms: (1) dechlorination and intramolecular cyclization can lead to the formation of HCl, benzene and naphthalene at 250-370 degrees C; (2) intermolecular crosslinking leads to the formation of alkyl aromatics such as toluene and xylene/ethylbenzene at 380-510 degrees C. The experimental results show that the combined ion source of SPI/ SPI-MEPEI for TOF-MS has broad application prospects in the online analysis field.

  6. Investigation of Volatiles Emitted from Freshly Cut Onions (Allium cepa L.) by Real Time Proton-Transfer Reaction-Mass Spectrometry (PTR-MS)

    PubMed Central

    Løkke, Mette Marie; Edelenbos, Merete; Larsen, Erik; Feilberg, Anders

    2012-01-01

    Volatile organic compounds (VOCs) in cut onions (Allium cepa L.) were continuously measured by PTR-MS during the first 120 min after cutting. The headspace composition changed rapidly due to the very reactive volatile sulfurous compounds emitted from onion tissue after cell disruption. Mass spectral signals corresponding to propanethial S-oxide (the lachrymatory factor) and breakdown products of this compound dominated 0–10 min after cutting. Subsequently, propanethiol and dipropyl disulfide predominantly appeared, together with traces of thiosulfinates. The concentrations of these compounds reached a maximum at 60 min after cutting. Propanethiol was present in highest concentrations and had an odor activity value 20 times higher than dipropyl disulfide. Thus, propanethiol is suggested to be the main source of the characteristic onion odor. Monitoring the rapid changes of VOCs in the headspace of cut onion necessitates a high time resolution, and PTR-MS is demonstrated to be a very suitable method for monitoring the headspace of freshly cut onions directly after cutting without extraction or pre-concentration. PMID:23443367

  7. Investigation of volatiles emitted from freshly cut onions (Allium cepa L.) by real time proton-transfer reaction-mass spectrometry (PTR-MS).

    PubMed

    Løkke, Mette Marie; Edelenbos, Merete; Larsen, Erik; Feilberg, Anders

    2012-11-22

    Volatile organic compounds (VOCs) in cut onions (Allium cepa L.) were continuously measured by PTR-MS during the first 120 min after cutting. The headspace composition changed rapidly due to the very reactive volatile sulfurous compounds emitted from onion tissue after cell disruption. Mass spectral signals corresponding to propanethial S-oxide (the lachrymatory factor) and breakdown products of this compound dominated 0-10 min after cutting. Subsequently, propanethiol and dipropyl disulfide predominantly appeared, together with traces of thiosulfinates. The concentrations of these compounds reached a maximum at 60 min after cutting. Propanethiol was present in highest concentrations and had an odor activity value 20 times higher than dipropyl disulfide. Thus, propanethiol is suggested to be the main source of the characteristic onion odor. Monitoring the rapid changes of VOCs in the headspace of cut onion necessitates a high time resolution, and PTR-MS is demonstrated to be a very suitable method for monitoring the headspace of freshly cut onions directly after cutting without extraction or pre-concentration.

  8. Real-time analysis of sulfur-containing volatiles in Brassica plants infested with root-feeding Delia radicum larvae using proton-transfer reaction mass spectrometry

    PubMed Central

    van Dam, Nicole M.; Samudrala, Devasena; Harren, Frans J. M.; Cristescu, Simona M

    2012-01-01

    Background and aims Plants damaged by herbivores emit a variety of volatile organic compounds (VOCs). Here we used proton-transfer reaction mass spectrometry (PTR-MS) as a sensitive detection method for online analysis of herbivore-induced VOCs. Previously, it was found that Brassica nigra plants emit several sulfur-containing VOCs when attacked by cabbage root fly (Delia radicum) larvae with m/z 60 as a marker for the formation of allylisothiocyanate from the glucosinolate sinigrin. We tested the hypothesis that m/z 60 emission occurs only in plants with sinigrin in their roots. Additionally, we tested the hypothesis that methanethiol, dimethylsulfide and dimethyldisulfide are only emitted after larval infestation. Methodology Proton-transfer reaction mass spectrometry was used to track sulfur-containing VOCs from six different species of Brassica over time. The roots were either artificially damaged or infested with cabbage root fly larvae. Glucosinolate profiles of the roots were analysed using high-pressure liquid chromatography and compared with VOC emissions. Principal results Brassica nigra, B. juncea and B. napus primarily emitted m/z 60 directly after artificial damage or root fly infestation. Sulfide and methanethiol emissions from B. nigra and B. juncea also increased after larval damage but much later (6–12 h after damage). Brassica rapa, B. oleracea and B. carinata principally emitted methanethiol after artificial and after larval damage. Brassica oleracea and B. carinata showed some increase in m/z 60 emission after larval damage. Comparison with root glucosinolate profiles revealed that sinigrin cannot be the only precursor for m/z 60. Conclusions The principal compound emitted after root damage is determined by the plant species, and not by damage type or root glucosinolate composition. Once determined, the principal compounds may be used as markers for identifying damaged or infested plants. Further analyses of plant enzymes involved in the

  9. Real-Time Tomography Mooring

    DTIC Science & Technology

    1992-06-01

    ma i t ain an accu racy of 20 jisec. far bet-ter than the I iiise accuracy (at b~est ) of’ thle tomograph11ic signal arrival t~ime deterininuat oion...surface buoy. The main supply consists of four stacks of ninety alkaline D-cells providing 360 amrlp hours of power at 12V to the IBC and associated...electronics. The second supply provides 90 amllp hours at 12V to the back-up PTT. The third supply is a rechargeable battery made up of lead-acid

  10. The ALMA Real Time Control System

    NASA Astrophysics Data System (ADS)

    Kern, Jeffrey S.; Juerges, Thomas A.; Marson, Ralph G.

    2009-01-01

    The Atacama Large Millimeter Array (ALMA) is a revolutionary millimeter and submillimeter array being developed on the Atacama plateau of northern Chile. An international partnership lead by NRAO, ESO, and NAOJ this powerful and flexible telescope will provide unprecedented observations of this relatively unexplored frequency range. The control subsystem for the Atacama Large Millimeter Array must coordinate the monitor and control of at least sixty six antennas (in four different styles), two correlators, and all of the ancillary equipment (samplers, local oscillators, front ends, etc.). This equipment will be spread over tens of kilometers and operated remotely. Operation of the array requires a robust, scalable, and maintainable real time control system. The real time control system is responsible for monitoring and control of any devices where there are fixed deadlines. Examples in the ALMA context are antenna pointing and fringe tracking. Traditionally the real time portion of a large software system is an intricate and error prone portion of the software. As a result the real time portion is very expensive in terms of effort expended both during construction and during maintenance phases of a project. The ALMA real time control system uses a Linux based real time operating system to interact with the hardware and the CORBA based ALMA Common Software to communicate in the distributed computing environment. Mixing the requirements of real time computing and the non-deterministic CORBA middleware has produced an interesting design. We discuss the architecture, design, and implementation of the ALMA real time control system. Highlight some lessons learned along the way, and justify our assertion that this should be the last large scale real time control system in radio astronomy.

  11. Real-time monitoring of landslides

    USGS Publications Warehouse

    Reid, Mark E.; LaHusen, Richard G.; Baum, Rex L.; Kean, Jason W.; Schulz, William H.; Highland, Lynn M.

    2012-01-01

    Landslides cause fatalities and property damage throughout the Nation. To reduce the impact from hazardous landslides, the U.S. Geological Survey develops and uses real-time and near-real-time landslide monitoring systems. Monitoring can detect when hillslopes are primed for sliding and can provide early indications of rapid, catastrophic movement. Continuous information from up-to-the-minute or real-time monitoring provides prompt notification of landslide activity, advances our understanding of landslide behavior, and enables more effective engineering and planning efforts.

  12. Achieving real-time performance in FIESTA

    NASA Technical Reports Server (NTRS)

    Wilkinson, William; Happell, Nadine; Miksell, Steve; Quillin, Robert; Carlisle, Candace

    1988-01-01

    The Fault Isolation Expert System for TDRSS Applications (FIESTA) is targeted for operation in a real-time online environment. Initial stages of the prototype development concentrated on acquisition and representation of the knowledge necessary to isolate faults in the TDRSS Network. Recent efforts focused on achieving real-time performance including: a discussion of the meaning of FIESTA real-time requirements, determination of performance levels (benchmarking) and techniques for optimization. Optimization techniques presented include redesign of critical relations, filtering of redundant data and optimization of patterns used in rules. Results are summarized.

  13. Real-time medical applications and telecommunications.

    PubMed

    Stravs, M

    1999-01-01

    Telecommunications play an important role in telemedicine. Many forms of telecommunication services based on different telecommunication technologies are developed for various needs. The paper deals with complex real-time applications which demand high telecommunication requirements. At the beginning, medical applications are categorised and real-time applications qualified as multimedia applications. Requirements for multimedia elements are listed separately. Later on, short introduction of related telecommunication protocols is given. Real-time medical applications can show their ability in case of guaranteed quality of services delivered by telecommunication network as it is explained in the end.

  14. Real time sensor for therapeutic radiation delivery

    DOEpatents

    Bliss, M.; Craig, R.A.; Reeder, P.L.

    1998-01-06

    The invention is a real time sensor for therapeutic radiation. A probe is placed in or near the patient that senses in real time the dose at the location of the probe. The strength of the dose is determined by either an insertion or an exit probe. The location is determined by a series of vertical and horizontal sensing elements that gives the operator a real time read out dose location relative to placement of the patient. The increased accuracy prevents serious tissue damage to the patient by preventing overdose or delivery of a dose to a wrong location within the body. 14 figs.

  15. Real time sensor for therapeutic radiation delivery

    DOEpatents

    Bliss, Mary; Craig, Richard A.; Reeder, Paul L.

    1998-01-01

    The invention is a real time sensor for therapeutic radiation. A probe is placed in or near the patient that senses in real time the dose at the location of the probe. The strength of the dose is determined by either an insertion or an exit probe. The location is determined by a series of vertical and horizontal sensing elements that gives the operator a real time read out dose location relative to placement of the patient. The increased accuracy prevents serious tissue damage to the patient by preventing overdose or delivery of a dose to a wrong location within the body.

  16. Spatial localisation of curcumin and rapid screening of the chemical compositions of turmeric rhizomes (Curcuma longa Linn.) using Direct Analysis in Real Time-Mass Spectrometry (DART-MS).

    PubMed

    Rahman, A F M Motiur; Angawi, Rihab F; Kadi, Adnan A

    2015-04-15

    Curcumin is a potent antioxidant agent having versatile biological activities is present in turmeric rhizomes (Curcuma longa Linn.). Powder of turmeric rhizomes is consumes as curry spicy worldwide, especially in Asia. In this study, we demonstrate that, bioactive curcumin and its analog demethoxycurcumin are chiefly concentrated in the pith rather than the other parts of the turmeric rhizomes and it was discovered using modern atmospheric ionisation source 'Direct Analysis in Real Time' (DART) connected with an Ion Trap Mass Spectrometry. In addition, all the major components present in turmeric rhizomes were detected in positive and/or in negative ion mode using DART.

  17. Quantitation of aflatoxins from corn and other food related materials by direct analysis in real time - mass spectrometry (DART-MS)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ambient ionization coupled to mass spectrometry continues to be applied to new analytical problems, facilitating the rapid and convenient analysis of a variety of analytes. Recently, demonstrations of ambient ionization mass spectrometry applied to quantitative analysis of mycotoxins have been shown...

  18. Characterization of a real-time tracer for isoprene epoxydiols-derived secondary organic aerosol (IEPOX-SOA) from aerosol mass spectrometer measurements

    NASA Astrophysics Data System (ADS)

    Hu, W. W.; Campuzano-Jost, P.; Palm, B. B.; Day, D. A.; Ortega, A. M.; Hayes, P. L.; Krechmer, J. E.; Chen, Q.; Kuwata, M.; Liu, Y. J.; de Sá, S. S.; McKinney, K.; Martin, S. T.; Hu, M.; Budisulistiorini, S. H.; Riva, M.; Surratt, J. D.; St. Clair, J. M.; Isaacman-Van Wertz, G.; Yee, L. D.; Goldstein, A. H.; Carbone, S.; Brito, J.; Artaxo, P.; de Gouw, J. A.; Koss, A.; Wisthaler, A.; Mikoviny, T.; Karl, T.; Kaser, L.; Jud, W.; Hansel, A.; Docherty, K. S.; Alexander, M. L.; Robinson, N. H.; Coe, H.; Allan, J. D.; Canagaratna, M. R.; Paulot, F.; Jimenez, J. L.

    2015-10-01

    Substantial amounts of secondary organic aerosol (SOA) can be formed from isoprene epoxydiols (IEPOX), which are oxidation products of isoprene mainly under low-NO conditions. Total IEPOX-SOA, which may include SOA formed from other parallel isoprene oxidation pathways, was quantified by applying positive matrix factorization (PMF) to aerosol mass spectrometer (AMS) measurements. The IEPOX-SOA fractions of organic aerosol (OA) in multiple field studies across several continents are summarized here and show consistent patterns with the concentration of gas-phase IEPOX simulated by the GEOS-Chem chemical transport model. During the Southern Oxidant and Aerosol Study (SOAS), 78 % of PMF-resolved IEPOX-SOA is accounted by the measured IEPOX-SOA molecular tracers (2-methyltetrols, C5-Triols, and IEPOX-derived organosulfate and its dimers), making it the highest level of molecular identification of an ambient SOA component to our knowledge. An enhanced signal at C5H6O+ (m/z 82) is found in PMF-resolved IEPOX-SOA spectra. To investigate the suitability of this ion as a tracer for IEPOX-SOA, we examine fC5H6O (fC5H6O= C5H6O+/OA) across multiple field, chamber, and source data sets. A background of ~ 1.7 ± 0.1 ‰ (‰ = parts per thousand) is observed in studies strongly influenced by urban, biomass-burning, and other anthropogenic primary organic aerosol (POA). Higher background values of 3.1 ± 0.6 ‰ are found in studies strongly influenced by monoterpene emissions. The average laboratory monoterpene SOA value (5.5 ± 2.0 ‰) is 4 times lower than the average for IEPOX-SOA (22 ± 7 ‰), which leaves some room to separate both contributions to OA. Locations strongly influenced by isoprene emissions under low-NO levels had higher fC5H6O (~ 6.5 ± 2.2 ‰ on average) than other sites, consistent with the expected IEPOX-SOA formation in those studies. fC5H6O in IEPOX-SOA is always elevated (12-40 ‰) but varies substantially between locations, which is shown to reflect

  19. Characterization of a real-time tracer for isoprene epoxydiols-derived secondary organic aerosol (IEPOX-SOA) from aerosol mass spectrometer measurements

    DOE PAGES

    Hu, W. W.; Campuzano-Jost, P.; Palm, B. B.; ...

    2015-10-23

    Substantial amounts of secondary organic aerosol (SOA) can be formed from isoprene epoxydiols (IEPOX), which are oxidation products of isoprene mainly under low-NO conditions. Total IEPOX-SOA, which may include SOA formed from other parallel isoprene oxidation pathways, was quantified by applying positive matrix factorization (PMF) to aerosol mass spectrometer (AMS) measurements. The IEPOX-SOA fractions of organic aerosol (OA) in multiple field studies across several continents are summarized here and show consistent patterns with the concentration of gas-phase IEPOX simulated by the GEOS-Chem chemical transport model. During the Southern Oxidant and Aerosol Study (SOAS), 78 % of PMF-resolved IEPOX-SOA is accountedmore » by the measured IEPOX-SOA molecular tracers (2-methyltetrols, C5-Triols, and IEPOX-derived organosulfate and its dimers), making it the highest level of molecular identification of an ambient SOA component to our knowledge. An enhanced signal at C5H6O+ (m/z 82) is found in PMF-resolved IEPOX-SOA spectra. To investigate the suitability of this ion as a tracer for IEPOX-SOA, we examine fC5H6O (fC5H6O= C5H6O+/OA) across multiple field, chamber, and source data sets. A background of ~ 1.7 ± 0.1 ‰ (‰ = parts per thousand) is observed in studies strongly influenced by urban, biomass-burning, and other anthropogenic primary organic aerosol (POA). Higher background values of 3.1 ± 0.6 ‰ are found in studies strongly influenced by monoterpene emissions. The average laboratory monoterpene SOA value (5.5 ± 2.0 ‰) is 4 times lower than the average for IEPOX-SOA (22 ± 7 ‰), which leaves some room to separate both contributions to OA. Locations strongly influenced by isoprene emissions under low-NO levels had higher fC5H6O (~ 6.5 ± 2.2 ‰ on average) than other sites, consistent with the expected IEPOX-SOA formation in those studies. fC5H6O in IEPOX-SOA is always elevated (12–40 ‰) but varies substantially between locations, which is shown

  20. Analysis of real-time vibration data

    USGS Publications Warehouse

    Safak, E.

    2005-01-01

    In recent years, a few structures have been instrumented to provide continuous vibration data in real time, recording not only large-amplitude motions generated by extreme loads, but also small-amplitude motions generated by ambient loads. The main objective in continuous recording is to track any changes in structural characteristics, and to detect damage after an extreme event, such as an earthquake or explosion. The Fourier-based spectral analysis methods have been the primary tool to analyze vibration data from structures. In general, such methods do not work well for real-time data, because real-time data are mainly composed of ambient vibrations with very low amplitudes and signal-to-noise ratios. The long duration, linearity, and the stationarity of ambient data, however, allow us to utilize statistical signal processing tools, which can compensate for the adverse effects of low amplitudes and high noise. The analysis of real-time data requires tools and techniques that can be applied in real-time; i.e., data are processed and analyzed while being acquired. This paper presents some of the basic tools and techniques for processing and analyzing real-time vibration data. The topics discussed include utilization of running time windows, tracking mean and mean-square values, filtering, system identification, and damage detection.

  1. Real-time scheduling using minimum search

    NASA Technical Reports Server (NTRS)

    Tadepalli, Prasad; Joshi, Varad

    1992-01-01

    In this paper we consider a simple model of real-time scheduling. We present a real-time scheduling system called RTS which is based on Korf's Minimin algorithm. Experimental results show that the schedule quality initially improves with the amount of look-ahead search and tapers off quickly. So it sppears that reasonably good schedules can be produced with a relatively shallow search.

  2. The LAA real-time benchmarks

    SciTech Connect

    Block, R.K.; Krischer, W.; Lone, S.

    1989-04-01

    In the context of the LAA detector development program a subgroup Real Time Data Processing has tackled the problem of intelligent triggering. The main goal of this group is to show how fast digital devices, implemented as custom-made or commercial processors, can execute some basic algorithms, and how they can be embedded in the data flow between detector readout components and fully programmable commercial processors, which are expected to be the final data processing filter in real time.

  3. Real-Time Ada Problem Study

    DTIC Science & Technology

    1989-03-24

    define this set of problems. The authors were chosen because of their proven expertise in real-time development with Ada. They could enrich the results of...for Real-Time Embedded Systems". LabTek Corporation, the author , had proven expertise in embedded system design utilizing Motorola MC680XO- based...processors. The second report is entitledSoftware Enineering Problems Using Ada in Computers Integral to Weapons Systems. Its author , Sonicraft, had

  4. REAL TIME SYSTEM OPERATIONS 2006-2007

    SciTech Connect

    Eto, Joseph H.; Parashar, Manu; Lewis, Nancy Jo

    2008-08-15

    The Real Time System Operations (RTSO) 2006-2007 project focused on two parallel technical tasks: (1) Real-Time Applications of Phasors for Monitoring, Alarming and Control; and (2) Real-Time Voltage Security Assessment (RTVSA) Prototype Tool. The overall goal of the phasor applications project was to accelerate adoption and foster greater use of new, more accurate, time-synchronized phasor measurements by conducting research and prototyping applications on California ISO's phasor platform - Real-Time Dynamics Monitoring System (RTDMS) -- that provide previously unavailable information on the dynamic stability of the grid. Feasibility assessment studies were conducted on potential application of this technology for small-signal stability monitoring, validating/improving existing stability nomograms, conducting frequency response analysis, and obtaining real-time sensitivity information on key metrics to assess grid stress. Based on study findings, prototype applications for real-time visualization and alarming, small-signal stability monitoring, measurement based sensitivity analysis and frequency response assessment were developed, factory- and field-tested at the California ISO and at BPA. The goal of the RTVSA project was to provide California ISO with a prototype voltage security assessment tool that runs in real time within California ISO?s new reliability and congestion management system. CERTS conducted a technical assessment of appropriate algorithms, developed a prototype incorporating state-of-art algorithms (such as the continuation power flow, direct method, boundary orbiting method, and hyperplanes) into a framework most suitable for an operations environment. Based on study findings, a functional specification was prepared, which the California ISO has since used to procure a production-quality tool that is now a part of a suite of advanced computational tools that is used by California ISO for reliability and congestion management.

  5. Effect of secondary organic aerosol coating thickness on the real-time detection and characterization of biomass-burning soot by two particle mass spectrometers

    NASA Astrophysics Data System (ADS)

    Ahern, Adam T.; Subramanian, Ramachandran; Saliba, Georges; Lipsky, Eric M.; Donahue, Neil M.; Sullivan, Ryan C.

    2016-12-01

    Biomass burning is a large source of light-absorbing refractory black carbon (rBC) particles with a wide range of morphologies and sizes. The net radiative forcing from these particles is strongly dependent on the amount and composition of non-light-absorbing material internally mixed with the rBC and on the morphology of the mixed particles. Understanding how the mixing state and morphology of biomass-burning aerosol evolves in the atmosphere is critical for constraining the influence of these particles on radiative forcing and climate. We investigated the response of two commercial laser-based particle mass spectrometers, the vacuum ultraviolet (VUV) ablation LAAPTOF and the IR vaporization SP-AMS, to monodisperse biomass-burning particles as we sequentially coated the particles with secondary organic aerosol (SOA) from α-pinene ozonolysis. We studied three mobility-selected soot core sizes, each with a number of successively thicker coatings of SOA applied. Using IR laser vaporization, the SP-AMS had different changes in sensitivity to rBC compared to potassium as a function of applied SOA coatings. We show that this is due to different effective beam widths for the IR laser vaporization region of potassium versus black carbon. The SP-AMS's sensitivity to black carbon (BC) mass was not observed to plateau following successive SOA coatings, despite achieving high OA : BC mass ratios greater than 9. We also measured the ion fragmentation pattern of biomass-burning rBC and found it changed only slightly with increasing SOA mass. The average organic matter ion signal measured by the LAAPTOF demonstrated a positive correlation with the condensed SOA mass on individual particles, despite the inhomogeneity of the particle core compositions. This demonstrates that the LAAPTOF can obtain quantitative mass measurements of aged soot-particle composition from realistic biomass-burning particles with complex morphologies and composition.

  6. Effect of secondary organic aerosol coating thickness on the real-time detection and characterization of biomass-burning soot by two particle mass spectrometers

    DOE PAGES

    Ahern, Adam T.; Subramanian, Ramachandran; Saliba, Georges; ...

    2016-12-22

    Biomass burning is a large source of light-absorbing refractory black carbon (rBC) particles with a wide range of morphologies and sizes. The net radiative forcing from these particles is strongly dependent on the amount and composition of non-light-absorbing material internally mixed with the rBC and on the morphology of the mixed particles. Understanding how the mixing state and morphology of biomass-burning aerosol evolves in the atmosphere is critical for constraining the influence of these particles on radiative forcing and climate. We investigated the response of two commercial laser-based particle mass spectrometers, the vacuum ultraviolet (VUV) ablation LAAPTOF and the IRmore » vaporization SP-AMS, to monodisperse biomass-burning particles as we sequentially coated the particles with secondary organic aerosol (SOA) from α-pinene ozonolysis. We studied three mobility-selected soot core sizes, each with a number of successively thicker coatings of SOA applied. Using IR laser vaporization, the SP-AMS had different changes in sensitivity to rBC compared to potassium as a function of applied SOA coatings. We show that this is due to different effective beam widths for the IR laser vaporization region of potassium versus black carbon. The SP-AMS's sensitivity to black carbon (BC) mass was not observed to plateau following successive SOA coatings, despite achieving high OA : BC mass ratios greater than 9. We also measured the ion fragmentation pattern of biomass-burning rBC and found it changed only slightly with increasing SOA mass. The average organic matter ion signal measured by the LAAPTOF demonstrated a positive correlation with the condensed SOA mass on individual particles, despite the inhomogeneity of the particle core compositions. This demonstrates that the LAAPTOF can obtain quantitative mass measurements of aged soot-particle composition from realistic biomass-burning particles with complex morphologies and composition.« less

  7. Real-time, high frequency QRS electrocardiograph

    NASA Technical Reports Server (NTRS)

    Schlegel, Todd T. (Inventor); DePalma, Jude L. (Inventor); Moradi, Saeed (Inventor)

    2006-01-01

    Real time cardiac electrical data are received from a patient, manipulated to determine various useful aspects of the ECG signal, and displayed in real time in a useful form on a computer screen or monitor. The monitor displays the high frequency data from the QRS complex in units of microvolts, juxtaposed with a display of conventional ECG data in units of millivolts or microvolts. The high frequency data are analyzed for their root mean square (RMS) voltage values and the discrete RMS values and related parameters are displayed in real time. The high frequency data from the QRS complex are analyzed with imbedded algorithms to determine the presence or absence of reduced amplitude zones, referred to herein as RAZs. RAZs are displayed as go, no-go signals on the computer monitor. The RMS and related values of the high frequency components are displayed as time varying signals, and the presence or absence of RAZs may be similarly displayed over time.

  8. Characterization of real-time computers

    NASA Technical Reports Server (NTRS)

    Shin, K. G.; Krishna, C. M.

    1984-01-01

    A real-time system consists of a computer controller and controlled processes. Despite the synergistic relationship between these two components, they have been traditionally designed and analyzed independently of and separately from each other; namely, computer controllers by computer scientists/engineers and controlled processes by control scientists. As a remedy for this problem, in this report real-time computers are characterized by performance measures based on computer controller response time that are: (1) congruent to the real-time applications, (2) able to offer an objective comparison of rival computer systems, and (3) experimentally measurable/determinable. These measures, unlike others, provide the real-time computer controller with a natural link to controlled processes. In order to demonstrate their utility and power, these measures are first determined for example controlled processes on the basis of control performance functionals. They are then used for two important real-time multiprocessor design applications - the number-power tradeoff and fault-masking and synchronization.

  9. Time of flight mass spectrometer

    DOEpatents

    Ulbricht, Jr., William H.

    1984-01-01

    A time-of-flight mass spectrometer is described in which ions are desorbed from a sample by nuclear fission fragments, such that desorption occurs at the surface of the sample impinged upon by the fission fragments. This configuration allows for the sample to be of any thickness, and eliminates the need for complicated sample preparation.

  10. Real-Time Gauge/Gravity Duality

    SciTech Connect

    Skenderis, Kostas; Rees, Balt C. van

    2008-08-22

    We present a general prescription for the holographic computation of real-time n-point functions in nontrivial states. In quantum field theory such real-time computations involve a choice of a time contour in the complex time plane. The holographic prescription amounts to 'filling in' this contour with bulk solutions: real segments of the contour are filled in with Lorentzian solutions while imaginary segments are filled in with Riemannian solutions and appropriate matching conditions are imposed at the corners of the contour. We illustrate the general discussion by computing the 2-point function of a scalar operator using this prescription and by showing that this leads to an unambiguous answer with the correct i{epsilon} insertions.

  11. Imaging of living cells in real time

    NASA Astrophysics Data System (ADS)

    Tychinsky, Vladimir P.; Koufal, Georgy E.; Perevedentseva, Elena V.; Vyshenskaia, Tatiana V.; Nikandrov, Serguei L.

    1996-12-01

    Parameters of intrinsic cell motility is one of the cell activity characteristics which can be measured in real-time. For evaluation of certain organelles velocity we propose to use high sensitivity of computer-aided phase microscope airyscan to local phase changes connected with refractive index. This method is based on periodical scanning of cell profile in direction perpendicular to organelles movement. Analysis of the obtained 2-dimensional time-coordinate matrix allows us to define organelle velocity in quasi-real time and areas of cell activity. The experiments with onion cells confirm the method applicability for cell activity investigation.

  12. Personnel real time dosimetry in interventional radiology.

    PubMed

    Servoli, L; Bissi, L; Fabiani, S; Magalotti, D; Placidi, P; Scorzoni, A; Calandra, A; Cicioni, R; Chiocchini, S; Dipilato, A C; Forini, N; Paolucci, M; Di Lorenzo, R; Cappotto, F P; Scarpignato, M; Maselli, A; Pentiricci, A

    2016-12-01

    Interventional radiology and hemodynamic procedures have rapidly grown in number in the past decade, increasing the importance of personnel dosimetry not only for patients but also for medical staff. The optimization of the absorbed dose during operations is one of the goals that fostered the development of real-time dosimetric systems. Indeed, introducing proper procedure optimization, like correlating dose rate measurements with medical staff position inside the operating room, the absorbed dose could be reduced. Real-time dose measurements would greatly facilitate this task through real-time monitoring and automatic data recording. Besides real-time dose monitoring could allow automatic data recording. In this work, we will describe the calibration and validation of a wireless real-time prototype dosimeter based on a new sensor device (CMOS imager). The validation measurement campaign in clinical conditions has demonstrated the prototype capability of measuring dose-rates with a frequency in the range of few Hz, and an uncertainty smaller than 10%.

  13. Real-time Enhanced Vision System

    NASA Technical Reports Server (NTRS)

    Hines, Glenn D.; Rahman, Zia-Ur; Jobson, Daniel J.; Woodell, Glenn A.; Harrah, Steven D.

    2005-01-01

    Flying in poor visibility conditions, such as rain, snow, fog or haze, is inherently dangerous. However these conditions can occur at nearly any location, so inevitably pilots must successfully navigate through them. At NASA Langley Research Center (LaRC), under support of the Aviation Safety and Security Program Office and the Systems Engineering Directorate, we are developing an Enhanced Vision System (EVS) that combines image enhancement and synthetic vision elements to assist pilots flying through adverse weather conditions. This system uses a combination of forward-looking infrared and visible sensors for data acquisition. A core function of the system is to enhance and fuse the sensor data in order to increase the information content and quality of the captured imagery. These operations must be performed in real-time for the pilot to use while flying. For image enhancement, we are using the LaRC patented Retinex algorithm since it performs exceptionally well for improving low-contrast range imagery typically seen during poor visibility conditions. In general, real-time operation of the Retinex requires specialized hardware. To date, we have successfully implemented a single-sensor real-time version of the Retinex on several different Digital Signal Processor (DSP) platforms. In this paper we give an overview of the EVS and its performance requirements for real-time enhancement and fusion and we discuss our current real-time Retinex implementations on DSPs.

  14. Visualization of Real-Time Data

    NASA Technical Reports Server (NTRS)

    Stansifer, Ryan; Engrand, Peter

    1996-01-01

    In this project we explored various approaches to presenting real-time data from the numerous systems monitored on the space shuttle to computer users. We examined the approach that several projects at the Kennedy Space Center (KSC) used to accomplish this. We undertook to build a prototype system to demonstrate that the Internet and the Java programming language could be used to present the real-time data conveniently. Several Java programs were developed that presented real-time data in different forms including one form that emulated the display screens of the PC GOAL system which is familiar to many at KSC. Also, we developed several communications programs to supply the data continuously. Furthermore, a framework was created using the World Wide Web (WWW) to organize the collection and presentation of the real-time data. We believe our demonstration project shows the great flexibility of the approach. We had no particular use of the data in mind, instead we wanted the most general and the least complex framework possible. People who wish to view data need only know how to use a WWW browser and the address (the URL). People wanting to build WWW documents containing real-time data need only know the values of a few parameters, they do not need to program in Java or any other language. These are stunning advantages over more monolithic systems.

  15. Feedback as Real-Time Constructions

    ERIC Educational Resources Information Center

    Keiding, Tina Bering; Qvortrup, Ane

    2014-01-01

    This article offers a re-description of feedback and the significance of time in feedback constructions based on systems theory. It describes feedback as internal, real-time constructions in a learning system. From this perspective, feedback is neither immediate nor delayed, but occurs in the very moment it takes place. This article argues for a…

  16. Making Real-Time Data "Real" for General Interest Users

    NASA Astrophysics Data System (ADS)

    Hotaling, L.

    2003-04-01

    Helping educators realize the benefits of integrating technology into curricula to effectively engage student learning and improve student achievement, particularly in science and mathematics, is the core mission of the Center for Improved Engineering and Science Education (CIESE). To achieve our mission, we focus on projects utilizing real-time data available from the Internet, and collaborative projects utilizing the Internet's potential to reach peers and experts around the world. As a member of the Mid-Atlantic Center for Ocean Sciences Education Excellence (COSEE), the Center for Improved Engineering and Science Education (CIESE), is committed to delivering relevant ocean science education to diverse audiences, including K-12 teachers, students, coastal managers, families and tourists. The highest priority of the Mid-Atlantic COSEE is to involve scientists and educators in the translation of data and information from the coastal observatories into instructional materials and products usable by educators and the public. A combination of three regional observing systems, the New Jersey Shelf Observing System (NJSOS), Chesapeake Bay Observing System (CBOS), and the York River observing system will provide the scientific backbone for an integrated program of science and education that improves user access to, and understanding of, modern ocean science and how it affects our daily lives. At present, the Mid-Atlantic COSEE offers three projects that enable users to apply and validate scientific concepts to real world situations. (1) The Gulf Stream Voyage is an online multidisciplinary project that utilizes both real-time data and primary source materials to help guide students to discover the science and history of the Gulf Stream current. (2) C.O.O.L. Classroom is an online project that utilizes concepts and real-time data collected through the NJSOS. The C.O.O.L. Classroom is based on the concept of the Rutgers-IMCS Coastal Ocean Observation Laboratory, a real

  17. Real-Time Visualization of Tissue Ischemia

    NASA Technical Reports Server (NTRS)

    Bearman, Gregory H. (Inventor); Chrien, Thomas D. (Inventor); Eastwood, Michael L. (Inventor)

    2000-01-01

    A real-time display of tissue ischemia which comprises three CCD video cameras, each with a narrow bandwidth filter at the correct wavelength is discussed. The cameras simultaneously view an area of tissue suspected of having ischemic areas through beamsplitters. The output from each camera is adjusted to give the correct signal intensity for combining with, the others into an image for display. If necessary a digital signal processor (DSP) can implement algorithms for image enhancement prior to display. Current DSP engines are fast enough to give real-time display. Measurement at three, wavelengths, combined into a real-time Red-Green-Blue (RGB) video display with a digital signal processing (DSP) board to implement image algorithms, provides direct visualization of ischemic areas.

  18. Real-time photo-magnetic imaging

    PubMed Central

    Nouizi, Farouk; Erkol, Hakan; Luk, Alex; Unlu, Mehmet B.; Gulsen, Gultekin

    2016-01-01

    We previously introduced a new high resolution diffuse optical imaging modality termed, photo-magnetic imaging (PMI). PMI irradiates the object under investigation with near-infrared light and monitors the variations of temperature using magnetic resonance thermometry (MRT). In this paper, we present a real-time PMI image reconstruction algorithm that uses analytic methods to solve the forward problem and assemble the Jacobian matrix much faster. The new algorithm is validated using real MRT measured temperature maps. In fact, it accelerates the reconstruction process by more than 250 times compared to a single iteration of the FEM-based algorithm, which opens the possibility for the real-time PMI. PMID:27867701

  19. Network protocols for real-time applications

    NASA Technical Reports Server (NTRS)

    Johnson, Marjory J.

    1987-01-01

    The Fiber Distributed Data Interface (FDDI) and the SAE AE-9B High Speed Ring Bus (HSRB) are emerging standards for high-performance token ring local area networks. FDDI was designed to be a general-purpose high-performance network. HSRB was designed specifically for military real-time applications. A workshop was conducted at NASA Ames Research Center in January, 1987 to compare and contrast these protocols with respect to their ability to support real-time applications. This report summarizes workshop presentations and includes an independent comparison of the two protocols. A conclusion reached at the workshop was that current protocols for the upper layers of the Open Systems Interconnection (OSI) network model are inadequate for real-time applications.

  20. Continuous, real time microwave plasma element sensor

    DOEpatents

    Woskov, Paul P.; Smatlak, Donna L.; Cohn, Daniel R.; Wittle, J. Kenneth; Titus, Charles H.; Surma, Jeffrey E.

    1995-01-01

    Microwave-induced plasma for continuous, real time trace element monitoring under harsh and variable conditions. The sensor includes a source of high power microwave energy and a shorted waveguide made of a microwave conductive, refractory material communicating with the source of the microwave energy to generate a plasma. The high power waveguide is constructed to be robust in a hot, hostile environment. It includes an aperture for the passage of gases to be analyzed and a spectrometer is connected to receive light from the plasma. Provision is made for real time in situ calibration. The spectrometer disperses the light, which is then analyzed by a computer. The sensor is capable of making continuous, real time quantitative measurements of desired elements, such as the heavy metals lead and mercury.

  1. Continuous, real time microwave plasma element sensor

    DOEpatents

    Woskov, P.P.; Smatlak, D.L.; Cohn, D.R.; Wittle, J.K.; Titus, C.H.; Surma, J.E.

    1995-12-26

    Microwave-induced plasma is described for continuous, real time trace element monitoring under harsh and variable conditions. The sensor includes a source of high power microwave energy and a shorted waveguide made of a microwave conductive, refractory material communicating with the source of the microwave energy to generate a plasma. The high power waveguide is constructed to be robust in a hot, hostile environment. It includes an aperture for the passage of gases to be analyzed and a spectrometer is connected to receive light from the plasma. Provision is made for real time in situ calibration. The spectrometer disperses the light, which is then analyzed by a computer. The sensor is capable of making continuous, real time quantitative measurements of desired elements, such as the heavy metals lead and mercury. 3 figs.

  2. Mobile waste inspection real time radiography system

    SciTech Connect

    Vigil, J.; Taggart, D.; Betts, S.; Rael, C.; Martinez, F.; Mendez, J.

    1995-10-01

    The 450-KeV Mobile Real Time Radiography System was designed and purchased to inspect containers of radioactive waste produced at Los Alamos National Laboratory (LANL). The Mobile Real Time Radiography System has the capability of inspecting waste containers of various sizes from 5-gal. buckets to standard waste boxes (SWB, dimensions 54.5 in. x 71 in. x 37 in.). The fact that this unit is mobile makes it an attractive alternative to the costly road closures associated with moving waste from the waste generator to storage or disposal facilities.

  3. Axial Tomography from Digitized Real Time Radiography

    DOE R&D Accomplishments Database

    Zolnay, A. S.; McDonald, W. M.; Doupont, P. A.; McKinney, R. L.; Lee, M. M.

    1985-01-18

    Axial tomography from digitized real time radiographs provides a useful tool for industrial radiography and tomography. The components of this system are: x-ray source, image intensifier, video camera, video line extractor and digitizer, data storage and reconstruction computers. With this system it is possible to view a two dimensional x-ray image in real time at each angle of rotation and select the tomography plane of interest by choosing which video line to digitize. The digitization of a video line requires less than a second making data acquisition relatively short. Further improvements on this system are planned and initial results are reported.

  4. Software Package For Real-Time Graphics

    NASA Technical Reports Server (NTRS)

    Malone, Jacqueline C.; Moore, Archie L.

    1991-01-01

    Software package for master graphics interactive console (MAGIC) at Western Aeronautical Test Range (WATR) of NASA Ames Research Center provides general-purpose graphical display system for real-time and post-real-time analysis of data. Written in C language and intended for use on workstation of interactive raster imaging system (IRIS) equipped with level-V Unix operating system. Enables flight researchers to create their own displays on basis of individual requirements. Applicable to monitoring of complicated processes in chemical industry.

  5. Machine vision for real time orbital operations

    NASA Technical Reports Server (NTRS)

    Vinz, Frank L.

    1988-01-01

    Machine vision for automation and robotic operation of Space Station era systems has the potential for increasing the efficiency of orbital servicing, repair, assembly and docking tasks. A machine vision research project is described in which a TV camera is used for inputing visual data to a computer so that image processing may be achieved for real time control of these orbital operations. A technique has resulted from this research which reduces computer memory requirements and greatly increases typical computational speed such that it has the potential for development into a real time orbital machine vision system. This technique is called AI BOSS (Analysis of Images by Box Scan and Syntax).

  6. Automated real-time software development

    NASA Technical Reports Server (NTRS)

    Jones, Denise R.; Walker, Carrie K.; Turkovich, John J.

    1993-01-01

    A Computer-Aided Software Engineering (CASE) system has been developed at the Charles Stark Draper Laboratory (CSDL) under the direction of the NASA Langley Research Center. The CSDL CASE tool provides an automated method of generating source code and hard copy documentation from functional application engineering specifications. The goal is to significantly reduce the cost of developing and maintaining real-time scientific and engineering software while increasing system reliability. This paper describes CSDL CASE and discusses demonstrations that used the tool to automatically generate real-time application code.

  7. Real-Time, Interactive Sonic Boom Display

    NASA Technical Reports Server (NTRS)

    Haering, Jr., Edward A. (Inventor); Plotkin, Kenneth J. (Inventor)

    2012-01-01

    The present invention is an improved real-time, interactive sonic boom display for aircraft. By using physical properties obtained via various sensors and databases, the invention determines, in real-time, sonic boom impacts locations and intensities for aircraft traveling at supersonic speeds. The information is provided to a pilot via a display that lists a selectable set of maneuvers available to the pilot to mitigate sonic boom issues. Upon selection of a maneuver, the information as to the result of the maneuver is displayed and the pilot may proceed with making the maneuver, or provide new data to the system in order to calculate a different maneuver.

  8. Real-Time Occupancy Change Analyzer

    SciTech Connect

    2005-03-30

    The Real-Time Occupancy Change Analyzer (ROCA) produces an occupancy grid map of an environment around the robot, scans the environment to generate a current obstacle map relative to a current robot position, and converts the current obstacle map to a current occupancy grid map. Changes in the occupancy grid can be reported in real time to support a number of tracking capabilities. The benefit of ROCA is that rather than only providing a vector to the detected change, it provides the actual x,y position of the change.

  9. Axial tomography from digitized real time radiography

    SciTech Connect

    Zolnay, A.S.; McDonald, W.M.; Doupont, P.A.; McKinney, R.L.; Lee, M.M.

    1985-01-18

    Axial tomography from digitized real time radiographs provides a useful tool for industrial radiography and tomography. The components of this system are: x-ray source, image intensifier, video camera, video line extractor and digitizer, data storage and reconstruction computers. With this system it is possible to view a two dimensional x-ray image in real time at each angle of rotation and select the tomography plane of interest by choosing which video line to digitize. The digitization of a video line requires less than a second making data acquisition relatively short. Further improvements on this system are planned and initial results are reported.

  10. Complete chemical analysis of aerosol particles in real-time

    SciTech Connect

    Yang, Mo; Reilly, P.T.A.; Gieray, R.A.; Whitten, W.B.; Ramsey, J.M.

    1996-12-31

    Real-time mass spectrometry of individual aerosol particles using an ion trap mass spectrometer is described. The microparticles are sampled directly from the air by a particle inlet system into the vacuum chamber. An incoming particle is detected as it passes through two CW laser beams and a pulsed laser is triggered to intercept the particle for laser ablation ionization at the center of the ion trap. The produced ions are analyzed by the ion trap mass spectrometer. Ions of interest are selected and dissociated through collision with buffer gas atoms for further fragmentation analysis. Real-time chemical analyses of inorganic, organic, and bacterial aerosol articles have been demonstrated. It has been confirmed that the velocity and the size of the incoming particles highly correlate to each other. The performance of the inlet system, particle detection, and preliminary results are discussed.

  11. OPAD-EDIFIS Real-Time Processing

    NASA Technical Reports Server (NTRS)

    Katsinis, Constantine

    1997-01-01

    The Optical Plume Anomaly Detection (OPAD) detects engine hardware degradation of flight vehicles through identification and quantification of elemental species found in the plume by analyzing the plume emission spectra in a real-time mode. Real-time performance of OPAD relies on extensive software which must report metal amounts in the plume faster than once every 0.5 sec. OPAD software previously written by NASA scientists performed most necessary functions at speeds which were far below what is needed for real-time operation. The research presented in this report improved the execution speed of the software by optimizing the code without changing the algorithms and converting it into a parallelized form which is executed in a shared-memory multiprocessor system. The resulting code was subjected to extensive timing analysis. The report also provides suggestions for further performance improvement by (1) identifying areas of algorithm optimization, (2) recommending commercially available multiprocessor architectures and operating systems to support real-time execution and (3) presenting an initial study of fault-tolerance requirements.

  12. Steerable real-time sonographically guided needle biopsy.

    PubMed

    Buonocore, E; Skipper, G J

    1981-02-01

    A method for dynamic real-time ultrasonic guidance for percutaneous needle biopsy has been successful in obtaining cytologic and histologic specimens from abdominal masses. The system depends on a real-time ultrasonic transducer that has been rigidly attached to a laterally placed steerable needle holder. Using simple trigonometric functions, a chart has been derived that gives the exact angulation and needle length to produce quick, reliable, guided needle placements. Examples of successful renal, hepatobiliary, and retroperitoneal biopsies are presented. Advantages of this technique include speed, accuracy, low cost, three-dimensional format, and the omission of contrast media and radiation.

  13. Method for analysis of psychopharmaceuticals in real industrial wastewater and groundwater with suspended organic particulate matter using solid phase extraction disks extraction and ultra-high performance liquid chromatography/time-of-flight mass spectrometry.

    PubMed

    Křesinová, Zdena; Linhartová, Lucie; Petrů, Klára; Krejčová, Lucie; Šrédlová, Kamila; Lhotský, Ondřej; Kameník, Zdeněk; Cajthaml, Tomáš

    2016-04-01

    A rapid and reliable analytical method was developed for the quantitative determination of psychopharmaceuticals, their precursors and by-products in real contaminated samples from a pharmaceutical company in Olomouc (Czech Republic), based on SPE disk extraction and detection by ultra performance liquid chromatography, combined with time-of-flight mass spectrometry. The target compounds were quantified in the real whole-water samples (water including suspended particles), both in the presence of suspended particulate matter (SPM) and high concentrations of other organic pollutants. A total of nine compounds were analyzed which consisted of three commonly used antidepressants (tricyclic antidepressants and antipsychotics), one antitussive agent and five by-products or precursors. At first, the SPE disk method was developed for the extraction of water samples (dissolved analytes, recovery 84-104%) and pressurised liquid extraction technique was verified for solid matrices (sludge samples, recovery 81-95%). In order to evaluate the SPE disk technique for whole water samples containing SPM, non contaminated groundwater samples were also loaded with different amounts (100 and 300mgL(-1)) of real contaminated sludge originating from the same locality. The recoveries from the whole-water samples obtained by SPE disk method ranged between 67 and 119% after the addition of the most contaminated sludge. The final method was applied to several real groundwater (whole-water) samples from the industrial area and high concentrations (up to 10(3)μgL(-1)) of the target compounds were detected. The results of this study document and indicate the feasibility of the SPE disk method for analysis of groundwater.

  14. Real-time analysis of telemetry data

    NASA Technical Reports Server (NTRS)

    Kao, Simon A.; Laffey, Thomas J.; Schmidt, James L.; Read, Jackson Y.; Dunham, Larry L.

    1987-01-01

    This paper descibes a knowledge-based system for performing real-time monitoring and analysis of telemetry data from the NASA Hubble Space Telescope (HST). In order to handle asynchronous inputs and perform in real time the system consists of three or more separate processes, which run concurrently and communicate via a message passing scheme. The data management process gathers, compresses, and scales the incoming telemetry data befoe sending it to the other tasks. The inferencing process uses the incoming data to perform a real-time analysis of the state and health of the Space Telescope. The I/O process receives telemetry monitors from the data management process, updates its graphical displays in real time, and acts as the interface to the console operator. The three processes may run on the same or different computers. This system is currently under development and is being used to monitor testcases produced by the Bass Telemetry System in the Hardware/Software Integration Facility at Lockheed Missile and Space Co. in Sunnyvale, California.

  15. The Power of Real-Time PCR

    ERIC Educational Resources Information Center

    Valasek, Mark A.; Repa, Joyce J.

    2005-01-01

    In recent years, real-time polymerase chain reaction (PCR) has emerged as a robust and widely used methodology for biological investigation because it can detect and quantify very small amounts of specific nucleic acid sequences. As a research tool, a major application of this technology is the rapid and accurate assessment of changes in gene…

  16. Real Time Grid Reliability Management 2005

    SciTech Connect

    Eto, Joe; Eto, Joe; Lesieutre, Bernard; Lewis, Nancy Jo; Parashar, Manu

    2008-07-07

    The increased need to manage California?s electricity grid in real time is a result of the ongoing transition from a system operated by vertically-integrated utilities serving native loads to one operated by an independent system operator supporting competitive energy markets. During this transition period, the traditional approach to reliability management -- construction of new transmission lines -- has not been pursued due to unresolved issues related to the financing and recovery of transmission project costs. In the absence of investments in new transmission infrastructure, the best strategy for managing reliability is to equip system operators with better real-time information about actual operating margins so that they can better understand and manage the risk of operating closer to the edge. A companion strategy is to address known deficiencies in offline modeling tools that are needed to ground the use of improved real-time tools. This project: (1) developed and conducted first-ever demonstrations of two prototype real-time software tools for voltage security assessment and phasor monitoring; and (2) prepared a scoping study on improving load and generator response models. Additional funding through two separate subsequent work authorizations has already been provided to build upon the work initiated in this project.

  17. Real-time distributed multimedia systems

    SciTech Connect

    Rahurkar, S.S.; Bourbakis, N.G.

    1996-12-31

    This paper presents a survey on distributed multimedia systems and discusses real-time issues. In particular, different subsystems are reviewed that impact on multimedia networking, the networking for multimedia, the networked multimedia systems, and the leading edge research and developments efforts and issues in networking.

  18. Real-time optoacoustic monitoring during thermotherapy

    NASA Astrophysics Data System (ADS)

    Esenaliev, Rinat O.; Larina, Irina V.; Larin, Kirill V.; Motamedi, Massoud

    2000-05-01

    Optoacoustic monitoring of tissue optical properties and speed of sound in real time can provide fast and accurate feedback information during thermotherapy performed with various heating or cooling agents. Amplitude and temporal characteristics of optoacoustic pressure waves are dependent on tissue properties. Detection and measurement of the optoacoustic waves may be used to monitor the extent of tissue hyperthermia, coagulation, or freezing with high resolution and contrast. We studied real-time optoacoustic monitoring of thermal coagulation induced by conductive heating and laser radiation and cryoablation with liquid nitrogen. Q-switched Nd:YAG laser pulses were used as probing radiation to induce optoacoustic waves in tissues. Dramatic changes in optoacoustic signal parameters were detected during tissue freezing and coagulation due to sharp changes in tissue properties. The dimensions of thermally- induced lesions were measured in real time with the optoacoustic technique. Our studies demonstrated that the laser optoacoustic technique is capable of real-time monitoring of tissue coagulation and freezing front with submillimeter spatial resolution. This may allow accurate thermal ablation or cryotherapy of malignant and benign lesions with minimal damage to normal tissues.

  19. REAL TIME CONTROL OF URBAN DRAINAGE NETWORKS

    EPA Science Inventory

    Real-time control (RTC) is a custom-designed, computer-assisted management technology for a specific sewerage network to meet the operational objectives of its collection/conveyance system. RTC can operate in several modes, including a mode that is activated during a wet weather ...

  20. ALMA Correlator Real-Time Data Processor

    NASA Astrophysics Data System (ADS)

    Pisano, J.; Amestica, R.; Perez, J.

    2005-10-01

    The design of a real-time Linux application utilizing Real-Time Application Interface (RTAI) to process real-time data from the radio astronomy correlator for the Atacama Large Millimeter Array (ALMA) is described. The correlator is a custom-built digital signal processor which computes the cross-correlation function of two digitized signal streams. ALMA will have 64 antennas with 2080 signal streams each with a sample rate of 4 giga-samples per second. The correlator's aggregate data output will be 1 gigabyte per second. The software is defined by hard deadlines with high input and processing data rates, while requiring interfaces to non real-time external computers. The designed computer system - the Correlator Data Processor or CDP, consists of a cluster of 17 SMP computers, 16 of which are compute nodes plus a master controller node all running real-time Linux kernels. Each compute node uses an RTAI kernel module to interface to a 32-bit parallel interface which accepts raw data at 64 megabytes per second in 1 megabyte chunks every 16 milliseconds. These data are transferred to tasks running on multiple CPUs in hard real-time using RTAI's LXRT facility to perform quantization corrections, data windowing, FFTs, and phase corrections for a processing rate of approximately 1 GFLOPS. Highly accurate timing signals are distributed to all seventeen computer nodes in order to synchronize them to other time-dependent devices in the observatory array. RTAI kernel tasks interface to the timing signals providing sub-millisecond timing resolution. The CDP interfaces, via the master node, to other computer systems on an external intra-net for command and control, data storage, and further data (image) processing. The master node accesses these external systems utilizing ALMA Common Software (ACS), a CORBA-based client-server software infrastructure providing logging, monitoring, data delivery, and intra-computer function invocation. The software is being developed in tandem

  1. Characterization and Quantification of Intact 26S Proteasome Proteins by Real-Time Measurement of Intrinsic Fluorescence Prior to Top-down Mass Spectrometry

    PubMed Central

    Russell, Jason D.; Scalf, Mark; Book, Adam J.; Ladror, Daniel T.; Vierstra, Richard D.; Smith, Lloyd M.; Coon, Joshua J.

    2013-01-01

    Quantification of gas-phase intact protein ions by mass spectrometry (MS) is impeded by highly-variable ionization, ion transmission, and ion detection efficiencies. Therefore, quantification of proteins using MS-associated techniques is almost exclusively done after proteolysis where peptides serve as proxies for estimating protein abundance. Advances in instrumentation, protein separations, and informatics have made large-scale sequencing of intact proteins using top-down proteomics accessible to the proteomics community; yet quantification of proteins using a top-down workflow has largely been unaddressed. Here we describe a label-free approach to determine the abundance of intact proteins separated by nanoflow liquid chromatography prior to MS analysis by using solution-phase measurements of ultraviolet light-induced intrinsic fluorescence (UV-IF). UV-IF is measured directly at the electrospray interface just prior to the capillary exit where proteins containing at least one tryptophan residue are readily detected. UV-IF quantification was demonstrated using commercially available protein standards and provided more accurate and precise protein quantification than MS ion current. We evaluated the parallel use of UV-IF and top-down tandem MS for quantification and identification of protein subunits and associated proteins from an affinity-purified 26S proteasome sample from Arabidopsis thaliana. We identified 26 unique proteins and quantified 13 tryptophan-containing species. Our analyses discovered previously unidentified N-terminal processing of the β6 (PBF1) and β7 (PBG1) subunit - such processing of PBG1 may generate a heretofore unknown additional protease active site upon cleavage. In addition, our approach permitted the unambiguous identification and quantification both isoforms of the proteasome-associated protein DSS1. PMID:23536786

  2. Real Time Radiation Monitoring Using Nanotechnology

    NASA Technical Reports Server (NTRS)

    Li, Jing (Inventor); Wilkins, Richard T. (Inventor); Hanratty, James J. (Inventor); Lu, Yijiang (Inventor)

    2016-01-01

    System and method for monitoring receipt and estimating flux value, in real time, of incident radiation, using two or more nanostructures (NSs) and associated terminals to provide closed electrical paths and to measure one or more electrical property change values .DELTA.EPV, associated with irradiated NSs, during a sequence of irradiation time intervals. Effects of irradiation, without healing and with healing, of the NSs, are separately modeled for first order and second order healing. Change values.DELTA.EPV are related to flux, to cumulative dose received by NSs, and to radiation and healing effectivity parameters and/or.mu., associated with the NS material and to the flux. Flux and/or dose are estimated in real time, based on EPV change values, using measured .DELTA.EPV values. Threshold dose for specified changes of biological origin (usually undesired) can be estimated. Effects of time-dependent radiation flux are analyzed in pre-healing and healing regimes.

  3. Real Time Radiation Exposure And Health Risks

    NASA Technical Reports Server (NTRS)

    Hu, Shaowen; Barzilla, Janet E.; Semones, Edward J.

    2015-01-01

    Radiation from solar particle events (SPEs) poses a serious threat to future manned missions outside of low Earth orbit (LEO). Accurate characterization of the radiation environment in the inner heliosphere and timely monitoring the health risks to crew are essential steps to ensure the safety of future Mars missions. In this project we plan to develop an approach that can use the particle data from multiple satellites and perform near real-time simulations of radiation exposure and health risks for various exposure scenarios. Time-course profiles of dose rates will be calculated with HZETRN and PDOSE from the energy spectrum and compositions of the particles archived from satellites, and will be validated from recent radiation exposure measurements in space. Real-time estimation of radiation risks will be investigated using ARRBOD. This cross discipline integrated approach can improve risk mitigation by providing critical information for risk assessment and medical guidance to crew during SPEs.

  4. Mass sociogenic illness--real and imaginary.

    PubMed

    Doyle, C R; Akhtar, J; Mrvos, R; Krenzelok, E P

    2004-04-01

    Mass sociogenic illness is the occurrence of a group of nonspecific physical symptoms for which no organic cause can be determined and is often transmitted by 'line of sight'. The fear of bioterrorism can also lead to panic and produce cases of mass sociogenic illness, in which people develop symptoms in response to an imaginary threat. Poison centers are faced with resolving the dilemma of sociogenic vs poison related symptoms. We report 2 situations of mass sociogenic illnesses involving school age children where multiple victims exhibited similar symptoms prompted by the presence or suggestion of fumes. Symptoms resolved spontaneously. When clusters of unexplained illness occur, a sociogenic etiology should be considered in the differential diagnosis. As fears about bioterrorism increase, the frequency of such incidents and the anxiety generated may increase.

  5. Nonlinear Real-Time Optical Signal Processing.

    DTIC Science & Technology

    1981-06-30

    bandwidth and space-bandwidth products. Real-time homonorphic and loga- rithmic filtering by halftone nonlinear processing has been achieved. A...Page ABSTRACT 1 1. RESEARCH OBJECTIVES AND PROGRESS 3 I-- 1.1 Introduction and Project overview 3 1.2 Halftone Processing 9 1.3 Direct Nonlinear...time homomorphic and logarithmic filtering by halftone nonlinear processing has been achieved. A detailed analysis of degradation due to the finite gamma

  6. Applications of real-time holography

    NASA Astrophysics Data System (ADS)

    Feinberg, J.

    1985-01-01

    Holographic principles are discussed, with a description of the steps of hologram production and reconstruction and the materials used in storage of the images. Real-time holography applications, including photolithography, double-exposure interferometry, time-averaged holography, parallel optical processing, optical phase conjugation, beam steering, and moving holograms, are described in detail. In addition, current areas of active research and some experimental applications are discussed.

  7. Real Time RF Simulator (RTS) and control

    SciTech Connect

    Cancelo, G.; Armiento, C.; Treptow, K.; Vignoni, A.; Zmuda, T.; /Fermilab

    2008-10-01

    The multi-cavity RTS allows LLRF algorithm development and lab testing prior to commissioning with real cavities and cryomodules. The RTS is a valuable tool since it models the functions, errors and disturbances of real RF systems. The advantage of a RTS over an off-line simulator is that it can be implemented on the actual LLRF hardware, on the same FPGA and processor, and run at the same speed of the LLRF control loop. Additionally the RTS can be shared by collaborators who do not have access to RF systems or when the systems are not available to LLRF engineers. The RTS simulator incorporates hardware, firmware and software errors and limitations of a real implementation, which would be hard to identify and time consuming to model in off-line simulations.

  8. Real Time Pricing and the Real Live Firm

    SciTech Connect

    Moezzi, Mithra; Goldman, Charles; Sezgen, Osman; Bharvirkar, Ranjit; Hopper, Nicole

    2004-05-26

    Energy economists have long argued the benefits of real time pricing (RTP) of electricity. Their basis for modeling customers response to short-term fluctuations in electricity prices are based on theories of rational firm behavior, where management strives to minimize operating costs and optimize profit, and labor, capital and energy are potential substitutes in the firm's production function. How well do private firms and public sector institutions operating conditions, knowledge structures, decision-making practices, and external relationships comport with these assumptions and how might this impact price response? We discuss these issues on the basis of interviews with 29 large (over 2 MW) industrial, commercial, and institutional customers in the Niagara Mohawk Power Corporation service territory that have faced day-ahead electricity market prices since 1998. We look at stories interviewees told about why and how they respond to RTP, why some customers report that they can't, and why even if they can, they don't. Some firms respond as theorized, and we describe their load curtailment strategies. About half of our interviewees reported that they were unable to either shift or forego electricity consumption even when prices are high ($0.50/kWh). Reasons customers gave for why they weren't price-responsive include implicit value placed on reliability, pricing structures, lack of flexibility in adjusting production inputs, just-in-time practices, perceived barriers to onsite generation, and insufficient time. We draw these observations into a framework that could help refine economic theory of dynamic pricing by providing real-world descriptions of how firms behave and why.

  9. Identification of Drugs in Parenteral Pharmaceutical Preparations from a Quality Assurance and a Diversion Program by Direct Analysis in Real-Time AccuTOFTM-Mass Spectrometry (DART-MS).

    PubMed

    Poklis, Justin L; Mohs, Amanda J; Wolf, Carl E; Poklis, Alphonse; Peace, Michelle R

    2016-10-01

    In healthcare settings drug diversion and impairment of physicians are major concerns requiring a rapid and efficient method for surveillance and detection. A Direct Analysis in Real Time ion source coupled to a JEOL AccuTOF(TM) time-of-flight mass spectrometer (DART-MS) method was developed to screen parenteral pharmaceutical formulations for potential drug diversion. Parenteral pharmaceutical formulations are also known as injectable formulations and are used with intravenous, subcutaneous, intramuscular and intra-articular administration. A library was created using the mass spectra data collected by a DART-MS operated in switching mode at 20, 60 and 90 V settings. This library contained 17 commonly encountered drugs in parenteral pharmaceutical formulations that included the surgical analgesic: fentanyl, hydromorphone and morphine; anesthetic: baclofen, bupivacaine, ketamine, midazolam, ropivacaine and succinylcholine; and a mixture of other drug classes: caffeine, clonidine, dexamethasone, ephedrine, heparin, methadone, oxytocin and phenylephrine. Randomly selected 200 de-identified parenteral pharmaceutical formulations containing one or more drugs were submitted for analysis to the FIRM Toxicology Laboratory at Virginia Commonwealth University Health and were screened using the DART-MS. The drug contents of the de-identified formulations were previously confirmed by a published high performance liquid chromatography (HPLC) method. The drugs in the formulations were rapidly and successfully identified using the generated library. The DART-MS and HPLC results were in complete agreement for all 200 parenteral pharmaceutical formulations.

  10. Real Time Correction of Aircraft Flight Fonfiguration

    NASA Technical Reports Server (NTRS)

    Schipper, John F. (Inventor)

    2009-01-01

    Method and system for monitoring and analyzing, in real time, variation with time of an aircraft flight parameter. A time-dependent recovery band, defined by first and second recovery band boundaries that are spaced apart at at least one time point, is constructed for a selected flight parameter and for a selected time recovery time interval length .DELTA.t(FP;rec). A flight parameter, having a value FP(t=t.sub.p) at a time t=t.sub.p, is likely to be able to recover to a reference flight parameter value FP(t';ref), lying in a band of reference flight parameter values FP(t';ref;CB), within a time interval given by t.sub.p.ltoreq.t'.ltoreq.t.sub.p.DELTA.t(FP;rec), if (or only if) the flight parameter value lies between the first and second recovery band boundary traces.

  11. Real time lobster posture estimation for behavior research

    NASA Astrophysics Data System (ADS)

    Yan, Sheng; Alfredsen, Jo Arve

    2017-02-01

    In animal behavior research, the main task of observing the behavior of an animal is usually done manually. The measurement of the trajectory of an animal and its real-time posture description is often omitted due to the lack of automatic computer vision tools. Even though there are many publications for pose estimation, few are efficient enough to apply in real-time or can be used without the machine learning algorithm to train a classifier from mass samples. In this paper, we propose a novel strategy for the real-time lobster posture estimation to overcome those difficulties. In our proposed algorithm, we use the Gaussian mixture model (GMM) for lobster segmentation. Then the posture estimation is based on the distance transform and skeleton calculated from the segmentation. We tested the algorithm on a serials lobster videos in different size and lighting conditions. The results show that our proposed algorithm is efficient and robust under various conditions.

  12. Real time gamma-ray signature identifier

    DOEpatents

    Rowland, Mark [Alamo, CA; Gosnell, Tom B [Moraga, CA; Ham, Cheryl [Livermore, CA; Perkins, Dwight [Livermore, CA; Wong, James [Dublin, CA

    2012-05-15

    A real time gamma-ray signature/source identification method and system using principal components analysis (PCA) for transforming and substantially reducing one or more comprehensive spectral libraries of nuclear materials types and configurations into a corresponding concise representation/signature(s) representing and indexing each individual predetermined spectrum in principal component (PC) space, wherein an unknown gamma-ray signature may be compared against the representative signature to find a match or at least characterize the unknown signature from among all the entries in the library with a single regression or simple projection into the PC space, so as to substantially reduce processing time and computing resources and enable real-time characterization and/or identification.

  13. Real time processor for array speckle interferometry

    NASA Technical Reports Server (NTRS)

    Chin, Gordon; Florez, Jose; Borelli, Renan; Fong, Wai; Miko, Joseph; Trujillo, Carlos

    1989-01-01

    The authors are constructing a real-time processor to acquire image frames, perform array flat-fielding, execute a 64 x 64 element two-dimensional complex FFT (fast Fourier transform) and average the power spectrum, all within the 25 ms coherence time for speckles at near-IR (infrared) wavelength. The processor will be a compact unit controlled by a PC with real-time display and data storage capability. This will provide the ability to optimize observations and obtain results on the telescope rather than waiting several weeks before the data can be analyzed and viewed with offline methods. The image acquisition and processing, design criteria, and processor architecture are described.

  14. Real-time measurements of jet aircraft engine exhaust.

    PubMed

    Rogers, Fred; Arnott, Pat; Zielinska, Barbara; Sagebiel, John; Kelly, Kerry E; Wagner, David; Lighty, JoAnn S; Sarofim, Adel F

    2005-05-01

    Particulate-phase exhaust properties from two different types of ground-based jet aircraft engines--high-thrust and turboshaft--were studied with real-time instruments on a portable pallet and additional time-integrated sampling devices. The real-time instruments successfully characterized rapidly changing particulate mass, light absorption, and polycyclic aromatic hydrocarbon (PAH) content. The integrated measurements included particulate-size distributions, PAH, and carbon concentrations for an entire test run (i.e., "run-integrated" measurements). In all cases, the particle-size distributions showed single modes peaking at 20-40nm diameter. Measurements of exhaust from high-thrust F404 engines showed relatively low-light absorption compared with exhaust from a turboshaft engine. Particulate-phase PAH measurements generally varied in phase with both net particulate mass and with light-absorbing particulate concentrations. Unexplained response behavior sometimes occurred with the real-time PAH analyzer, although on average the real-time and integrated PAH methods agreed within the same order of magnitude found in earlier investigations.

  15. Real-Time Seismology in Portugal

    NASA Astrophysics Data System (ADS)

    Custodio, S.; Marreiros, C.; Carvalho, S.; Vales, D.; Lima, V.; Carrilho, F.

    2012-12-01

    Portugal is located next to the plate boundary between Eurasia (Iberia) and Africa (Nubia). The country has been repeatedly affected by some of the largest earthquakes, both onshore and offshore, in the historical European record, including the largest historical European earthquake, the great Lisbon earthquake of 1755 (~M8.5). The Portuguese territory has suffered directly the consequences of strong ground shaking (collapse of buildings, etc) and also some of the most destructive consequences of earthquakes (e.g. tsunamis, fires, etc). However, the rate of tectonic deformation in the Portuguese territory is low (the Eurasian-African plates converge at a rate of ~ 5 mm/yr), which results in long recurrence intervals between earthquakes. This low to moderate rate of seismic activity has two major negative effects: 1) it is difficult to study the regional seismo-tectonics with traditional passive methods; 2) the population is little aware of earthquake risk and unprepared to react in case of disaster. In this scenario, real-time seismology is key to monitoring earthquake crisis in real-time, providing early warnings about potentially destructive events, and assisting in the channeling of recovery efforts in case of disaster. In this paper we will present the real-time algorithms implemented at Instituto de Meteorologia (IM), the institution responsible for seismic monitoring in Portugal. In particular, we will focus on the following aspects: 1) Data collection and real-time transmission to the headquarters. Broadband seismological stations are owned and operated by five different institutions. The last years have witnessed an effort for integration, and presently most data arrives at IM lab in real-time. 2) Earthquake location and local magnitude determination. Data is automatically analyzed in order to obtain a first earthquake hypocenter and ML. While this process is mostly automatic, it still requires the revision by an operator, who is available 24h. 3

  16. Real-time video image processing

    NASA Astrophysics Data System (ADS)

    Smedley, Kirk G.; Yool, Stephen R.

    1990-11-01

    Lockheed has designed and implemented a prototype real-time Video Enhancement Workbench (VEW) using commercial offtheshelf hardware and custom software. The hardware components include a Sun workstation Aspex PIPE image processor time base corrector VCR video camera and realtime disk subsystem. A cornprehensive set of image processing functions can be invoked by the analyst at any time during processing enabling interactive enhancement and exploitation of video sequences. Processed images can be transmitted and stored within the system in digital or video form. VEW also provides image output to a laser printer and to Interleaf technical publishing software.

  17. Real-time remote scientific model validation

    NASA Technical Reports Server (NTRS)

    Frainier, Richard; Groleau, Nicolas

    1994-01-01

    This paper describes flight results from the use of a CLIPS-based validation facility to compare analyzed data from a space life sciences (SLS) experiment to an investigator's preflight model. The comparison, performed in real-time, either confirms or refutes the model and its predictions. This result then becomes the basis for continuing or modifying the investigator's experiment protocol. Typically, neither the astronaut crew in Spacelab nor the ground-based investigator team are able to react to their experiment data in real time. This facility, part of a larger science advisor system called Principal Investigator in a Box, was flown on the space shuttle in October, 1993. The software system aided the conduct of a human vestibular physiology experiment and was able to outperform humans in the tasks of data integrity assurance, data analysis, and scientific model validation. Of twelve preflight hypotheses associated with investigator's model, seven were confirmed and five were rejected or compromised.

  18. AMON: Transition to real-time operations

    NASA Astrophysics Data System (ADS)

    Cowen, D. F.; Keivani, A.; Tešić, G.

    2016-04-01

    The Astrophysical Multimessenger Observatory Network (AMON) will link the world's leading high-energy neutrino, cosmic-ray, gamma-ray and gravitational wave observatories by performing real-time coincidence searches for multimessenger sources from observatories' subthreshold data streams. The resulting coincidences will be distributed to interested parties in the form of electronic alerts for real-time follow-up observation. We will present the science case, design elements, current and projected partner observatories, status of the AMON project, and an initial AMON-enabled analysis. The prototype of the AMON server has been online since August 2014 and processing archival data. Currently, we are deploying new high-uptime servers and will be ready to start issuing alerts as early as winter 2015/16.

  19. System Equivalent for Real Time Digital Simulator

    NASA Astrophysics Data System (ADS)

    Lin, Xi

    2011-07-01

    The purpose of this research is to develop a method of making system equivalents for the Real Time Digital Simulator (RTDS), which should enhance its capability of simulating large power systems. The proposed equivalent combines a Frequency Dependent Network Equivalent (FDNE) for the high frequency electromagnetic transients and a Transient Stability Analysis (TSA) type simulation block for the electromechanical transients. The frequency dependent characteristic for FDNE is obtained by curve-fitting frequency domain admittance characteristics using the Vector Fitting method. An approach for approximating the frequency dependent characteristic of large power networks from readily available typical power-flow data is also introduced. A new scheme of incorporating TSA solution in RTDS is proposed. This report shows how the TSA algorithm can be adapted to a real time platform. The validity of this method is confirmed with examples, including the study of a multi in-feed HVDC system based network.

  20. Distributed Real-Time Computing with Harness

    SciTech Connect

    Di Saverio, Emanuele; Cesati, Marco; Di Biagio, Christian; Pennella, Guido; Engelmann, Christian

    2007-01-01

    Modern parallel and distributed computing solutions are often built onto a ''middleware'' software layer providing a higher and common level of service between computational nodes. Harness is an adaptable, plugin-based middleware framework for parallel and distributed computing. This paper reports recent research and development results of using Harness for real-time distributed computing applications in the context of an industrial environment with the needs to perform several safety critical tasks. The presented work exploits the modular architecture of Harness in conjunction with a lightweight threaded implementation to resolve several real-time issues by adding three new Harness plug-ins to provide a prioritized lightweight execution environment, low latency communication facilities, and local timestamped event logging.

  1. Real-time imaging of quantum entanglement.

    PubMed

    Fickler, Robert; Krenn, Mario; Lapkiewicz, Radek; Ramelow, Sven; Zeilinger, Anton

    2013-01-01

    Quantum Entanglement is widely regarded as one of the most prominent features of quantum mechanics and quantum information science. Although, photonic entanglement is routinely studied in many experiments nowadays, its signature has been out of the grasp for real-time imaging. Here we show that modern technology, namely triggered intensified charge coupled device (ICCD) cameras are fast and sensitive enough to image in real-time the effect of the measurement of one photon on its entangled partner. To quantitatively verify the non-classicality of the measurements we determine the detected photon number and error margin from the registered intensity image within a certain region. Additionally, the use of the ICCD camera allows us to demonstrate the high flexibility of the setup in creating any desired spatial-mode entanglement, which suggests as well that visual imaging in quantum optics not only provides a better intuitive understanding of entanglement but will improve applications of quantum science.

  2. Neural Network Retinal Model Real Time Implementation

    DTIC Science & Technology

    1992-09-02

    addresses the specific needs of vision processing. The goal of this SBIR Phase I project has been to take a significant neural network vision...application and to map it onto dedicated hardware for real time implementation. The neural network was already demonstrated using software simulation on a...general purpose computer. During Phase 1, HNC took a neural network model of the retina and, using HNC’s Vision Processor (ViP) prototype hardware

  3. Real-Time Ocean Modeling Systems

    DTIC Science & Technology

    2013-10-22

    2002 2. REPORT TYPE 3. DATES COVERED (From - To) Journal Article 4 . TITLE AND SUBTITLE Real-time 16iebaf Modeling Systems \\&&»A 5a...Director NCST E.O. Hartwig, 7000 Public Affairs (Unclassified/ Unlimited Only), Code 7n30 4 Division, Code Author, Code HQ-NRL 5511/6 (Rev. 12-93...according to the routing in Section 4 . 1. NRL Reports Submit the diskette (if available), manuscript, typed double-spaced, complete with tables

  4. Real-Time X-Ray Inspection

    NASA Technical Reports Server (NTRS)

    Bulthuis, Ronald V.

    1988-01-01

    X-ray imaging instrument adapted to continuous scanning. Modern version of fluoroscope enables rapid x-ray inspection of parts. Developed for detection of buckling in insulated ducts. Uses radiation from radioactive gadolinium or thallium source. Instrument weighs only 6 1/2 lb. Quickly scanned by hand along duct surface, providing real-time image. Based on Lixiscope, developed at Goddard Space Flight Center.

  5. Nonlinear Real-Time Optical Signal Processing.

    DTIC Science & Technology

    1984-10-01

    DTIC ELECTE I B IIMAGE PROCESSING INSTITUTE 84 11 26 107 UNCLASSIFIED SECURITY CLASSIFICATION OF THIS PAGE (When Dota Entered), REPORT DOCUMENTATION...30, 1984 N NONLINEAR REAL-TIME OPTICAL SIGNAL PROCESSING i E~ A.A. Sawchuk, Principal Investigator T.C. Strand and A.R. Tanguay. Jr. October 1, 1984...RDepartment of Electrical Engineering Image Processing institute University of Southern California University Park-MC 0272 Los Angeles, California

  6. Software Analyzes Complex Systems in Real Time

    NASA Technical Reports Server (NTRS)

    2008-01-01

    Expert system software programs, also known as knowledge-based systems, are computer programs that emulate the knowledge and analytical skills of one or more human experts, related to a specific subject. SHINE (Spacecraft Health Inference Engine) is one such program, a software inference engine (expert system) designed by NASA for the purpose of monitoring, analyzing, and diagnosing both real-time and non-real-time systems. It was developed to meet many of the Agency s demanding and rigorous artificial intelligence goals for current and future needs. NASA developed the sophisticated and reusable software based on the experience and requirements of its Jet Propulsion Laboratory s (JPL) Artificial Intelligence Research Group in developing expert systems for space flight operations specifically, the diagnosis of spacecraft health. It was designed to be efficient enough to operate in demanding real time and in limited hardware environments, and to be utilized by non-expert systems applications written in conventional programming languages. The technology is currently used in several ongoing NASA applications, including the Mars Exploration Rovers and the Spacecraft Health Automatic Reasoning Pilot (SHARP) program for the diagnosis of telecommunication anomalies during the Neptune Voyager Encounter. It is also finding applications outside of the Space Agency.

  7. Real-time Interactive Tree Animation.

    PubMed

    Quigley, Ed; Yu, Yue; Huang, Jingwei; Lin, Winnie; Fedkiw, Ronald

    2017-01-30

    We present a novel method for posing and animating botanical tree models interactively in real time. Unlike other state of the art methods which tend to produce trees that are overly flexible, bending and deforming as if they were underwater plants, our approach allows for arbitrarily high stiffness while still maintaining real-time frame rates without spurious artifacts, even on quite large trees with over ten thousand branches. This is accomplished by using an articulated rigid body model with as-stiff-as-desired rotational springs in conjunction with our newly proposed simulation technique, which is motivated both by position based dynamics and the typical O(N) algorithms for articulated rigid bodies. The efficiency of our algorithm allows us to pose and animate trees with millions of branches or alternatively simulate a small forest comprised of many highly detailed trees. Even using only a single CPU core, we can simulate ten thousand branches in real time while still maintaining quite crisp user interactivity. This has allowed us to incorporate our framework into a commodity game engine to run interactively even on a low-budget tablet. We show that our method is amenable to the incorporation of a large variety of desirable effects such as wind, leaves, fictitious forces, collisions, fracture, etc.

  8. Real-time monitoring system for microfluidics

    NASA Astrophysics Data System (ADS)

    Sapuppo, F.; Cantelli, G.; Fortuna, L.; Arena, P.; Bucolo, M.

    2007-05-01

    A new non-invasive real-time system for the monitoring and control of microfluidodynamic phenomena is proposed. The general purpose design of such system is suitable for in vitro and in vivo experimental setup and therefore for microfluidic application in the biomedical field such as lab-on-chip and for research studies in the field of microcirculation. The system consists of an ad hoc optical setup for image magnification providing images suitable for image acquisition and processing. The optic system was designed and developed using discrete opto-mechanic components mounted on a breadboard in order to provide an optic path accessible at any point where the information needs to be acquired. The optic sensing, acquisition, and processing were performed using an integrated vision system based on the Cellular Nonlinear Networks (CNNs) analogic technology called Focal Plane Processor (FPP, Eye-RIS, Anafocus) and inserted in the optic path. Ad hoc algorithms were implemented for the real-time analysis and extraction of fluido-dynamic parameters in micro-channels. They were tested on images recorded during in vivo microcirculation experiments on hamsters and then they were applied on images optically acquired and processed in real-time during in vitro experiments on a continuous microfluidic device (serpentine mixer, ThinXXS) with a two-phase fluid.

  9. Real-time speckle photography: a breakthrough

    NASA Astrophysics Data System (ADS)

    Petrov, Valery

    1996-12-01

    Speckle photography for small displacements can be carried out rather easily. It is a well established method. Unfortunately problems arise when specklegrams must be obtained in real time. Silver halide media infer lengthy multi-stage photoprocessing of specklegrams. Real time speckle photography utilizing non-silver media can be implemented but the techniques involved are rather complicated. Extremely simple and inexpensive approaches to speckle photography are introduced here. They combine positive features of speckle photography and momental holography. This permits the user to produce quasi real time specklegrams within a few seconds. High quality speckle photographs were obtained with different laser sources on high resolution silver halide media: Russian PFG-03, PFG-03 C (color), Agfa-Gevaert 8E 75 HD films and plates. Very good specklegrams were obtained also in lighted environment. Hybrid holospecklegrams i.e. holograms and speckle photographs of the same object were obtained simultaneously on the same media. Such holospecklegrams were also produced within fa few seconds. Quite unexpectedly good specklegrams were recorded even in water. Photographs of momentally produced specklegrams are given.

  10. Real-time preprocessing of holographic information

    NASA Astrophysics Data System (ADS)

    Schilling, Bradley W.; Poon, Ting-Chung

    1995-11-01

    Optical scanning holography (OSH) is a holographic recording technique that uses active optical heterodyne scanning to generate holographic information pertaining to an object. The holographic information manifests itself as an electrical signal suitable for real-time image reconstruction using a spatial light modulator. The electrical signal that carries the holographic information can also be digitized for computer storage and processing, allowing the image reconstruction to be performed numerically. In previous experiments with this technique, holographic information has been recorded using the interference pattern of a plane wave and a spherical wave of different temporal frequencies to scan an object. However, the proper manipulation of the pupil functions in the recording stage can result in real-time processing of the holographic edge extraction technique as an important example of real-time preprocessing of holographic information that utilizes alternate pupils in the OSH recording stage. We investigate the theory of holographic preprocessing using a spatial frequency-domain analysis based on the recording system's optical transfer function. The theory is reinforced through computer simulation.

  11. Real-time optical image processing techniques

    NASA Technical Reports Server (NTRS)

    Liu, Hua-Kuang

    1988-01-01

    Nonlinear real-time optical processing on spatial pulse frequency modulation has been pursued through the analysis, design, and fabrication of pulse frequency modulated halftone screens and the modification of micro-channel spatial light modulators (MSLMs). Micro-channel spatial light modulators are modified via the Fabry-Perot method to achieve the high gamma operation required for non-linear operation. Real-time nonlinear processing was performed using the halftone screen and MSLM. The experiments showed the effectiveness of the thresholding and also showed the needs of higher SBP for image processing. The Hughes LCLV has been characterized and found to yield high gamma (about 1.7) when operated in low frequency and low bias mode. Cascading of two LCLVs should also provide enough gamma for nonlinear processing. In this case, the SBP of the LCLV is sufficient but the uniformity of the LCLV needs improvement. These include image correlation, computer generation of holograms, pseudo-color image encoding for image enhancement, and associative-retrieval in neural processing. The discovery of the only known optical method for dynamic range compression of an input image in real-time by using GaAs photorefractive crystals is reported. Finally, a new architecture for non-linear multiple sensory, neural processing has been suggested.

  12. Machine learning for real time remote detection

    NASA Astrophysics Data System (ADS)

    Labbé, Benjamin; Fournier, Jérôme; Henaff, Gilles; Bascle, Bénédicte; Canu, Stéphane

    2010-10-01

    Infrared systems are key to providing enhanced capability to military forces such as automatic control of threats and prevention from air, naval and ground attacks. Key requirements for such a system to produce operational benefits are real-time processing as well as high efficiency in terms of detection and false alarm rate. These are serious issues since the system must deal with a large number of objects and categories to be recognized (small vehicles, armored vehicles, planes, buildings, etc.). Statistical learning based algorithms are promising candidates to meet these requirements when using selected discriminant features and real-time implementation. This paper proposes a new decision architecture benefiting from recent advances in machine learning by using an effective method for level set estimation. While building decision function, the proposed approach performs variable selection based on a discriminative criterion. Moreover, the use of level set makes it possible to manage rejection of unknown or ambiguous objects thus preserving the false alarm rate. Experimental evidences reported on real world infrared images demonstrate the validity of our approach.

  13. Acting to gain information: Real-time reasoning meets real-time perception

    NASA Technical Reports Server (NTRS)

    Rosenschein, Stan

    1994-01-01

    Recent advances in intelligent reactive systems suggest new approaches to the problem of deriving task-relevant information from perceptual systems in real time. The author will describe work in progress aimed at coupling intelligent control mechanisms to real-time perception systems, with special emphasis on frame rate visual measurement systems. A model for integrated reasoning and perception will be discussed, and recent progress in applying these ideas to problems of sensor utilization for efficient recognition and tracking will be described.

  14. Exploring Earthquakes in Real-Time

    NASA Astrophysics Data System (ADS)

    Bravo, T. K.; Kafka, A. L.; Coleman, B.; Taber, J. J.

    2013-12-01

    Earthquakes capture the attention of students and inspire them to explore the Earth. Adding the ability to view and explore recordings of significant and newsworthy earthquakes in real-time makes the subject even more compelling. To address this opportunity, the Incorporated Research Institutions for Seismology (IRIS), in collaboration with Moravian College, developed ';jAmaSeis', a cross-platform application that enables students to access real-time earthquake waveform data. Students can watch as the seismic waves are recorded on their computer, and can be among the first to analyze the data from an earthquake. jAmaSeis facilitates student centered investigations of seismological concepts using either a low-cost educational seismograph or streamed data from other educational seismographs or from any seismic station that sends data to the IRIS Data Management System. After an earthquake, students can analyze the seismograms to determine characteristics of earthquakes such as time of occurrence, distance from the epicenter to the station, magnitude, and location. The software has been designed to provide graphical clues to guide students in the analysis and assist in their interpretations. Since jAmaSeis can simultaneously record up to three stations from anywhere on the planet, there are numerous opportunities for student driven investigations. For example, students can explore differences in the seismograms from different distances from an earthquake and compare waveforms from different azimuthal directions. Students can simultaneously monitor seismicity at a tectonic plate boundary and in the middle of the plate regardless of their school location. This can help students discover for themselves the ideas underlying seismic wave propagation, regional earthquake hazards, magnitude-frequency relationships, and the details of plate tectonics. The real-time nature of the data keeps the investigations dynamic, and offers students countless opportunities to explore.

  15. Real-Time Flight Envelope Monitoring System

    NASA Technical Reports Server (NTRS)

    Kerho, Michael; Bragg, Michael B.; Ansell, Phillip J.

    2012-01-01

    The objective of this effort was to show that real-time aircraft control-surface hinge-moment information could be used to provide a robust and reliable prediction of vehicle performance and control authority degradation. For a given airfoil section with a control surface -- be it a wing with an aileron, rudder, or elevator -- the control-surface hinge moment is sensitive to the aerodynamic characteristics of the section. As a result, changes in the aerodynamics of the section due to angle-of-attack or environmental effects such as icing, heavy rain, surface contaminants, bird strikes, or battle damage will affect the control surface hinge moment. These changes include both the magnitude of the hinge moment and its sign in a time-averaged sense, and the variation of the hinge moment with time. The current program attempts to take the real-time hinge moment information from the aircraft control surfaces and develop a system to predict aircraft envelope boundaries across a range of conditions, alerting the flight crew to reductions in aircraft controllability and flight boundaries.

  16. Real-time design with peer tasks

    NASA Technical Reports Server (NTRS)

    Goforth, Andre; Howes, Norman R.; Wood, Jonathan D.; Barnes, Michael J.

    1995-01-01

    We introduce a real-time design methodology for large scale, distributed, parallel architecture, real-time systems (LDPARTS), as an alternative to those methods using rate or dead-line monotonic analysis. In our method the fundamental units of prioritization, work items, are domain specific objects with timing requirements (deadlines) found in user's specification. A work item consists of a collection of tasks of equal priority. Current scheduling theories are applied with artifact deadlines introduced by the designer whereas our method schedules work items to meet user's specification deadlines (sometimes called end-to-end deadlines). Our method supports these scheduling properties. Work item scheduling is based on domain specific importance instead of task level urgency and still meets as many user specification deadlines as can be met by scheduling tasks with respect to urgency. Second, the minimum (closest) on-line deadline that can be guaranteed for a work item of highest importance, scheduled at run time, is approximately the inverse of the throughput, measured in work items per second. Third, throughput is not degraded during overload and instead of resorting to task shedding during overload, the designer can specify which work items to shed. We prove these properties in a mathematical model.

  17. Solid-phase extraction with the metal-organic framework MIL-101(Cr) combined with direct analysis in real time mass spectrometry for the fast analysis of triazine herbicides.

    PubMed

    Li, Xianjiang; Xing, Jiawei; Chang, Cuilan; Wang, Xin; Bai, Yu; Yan, Xiuping; Liu, Huwei

    2014-06-01

    MIL-101(Cr) is an excellent metal-organic framework with high surface area and nanoscale cavities, making it promising in solid-phase extraction. Herein, we used MIL-101(Cr) as a solid-phase extraction packing material combined with fast detection of direct analysis in real time mass spectrometry (DART-MS) for the analysis of triazine herbicides. After systematic optimization of the operation parameters, including the gas temperature of DART, the moving speed of the 1D platform, solvent for desorption, amount of MIL-101(Cr) extraction time, eluent volume and salt concentration, this method can realize the simultaneous detection of five kinds of triazine herbicides. The limits of detection were 0.1∼0.2 ng/mL and the linear ranges covered more than two orders of magnitude with the quantitation limits of 0.5∼1 ng/mL. Moreover, the developed method has been applied for the analysis of lake water samples and the recoveries for spiked analytes were in the range of 85∼110%. These results showed that solid-phase extraction with metal-organic frameworks is an efficient sample preparation approach for DART-MS analysis and could find more applications in environmental analysis.

  18. Online coupling of in-tube solid-phase microextraction with direct analysis in real time mass spectrometry for rapid determination of triazine herbicides in water using carbon-nanotubes-incorporated polymer monolith.

    PubMed

    Wang, Xin; Li, Xianjiang; Li, Ze; Zhang, Yiding; Bai, Yu; Liu, Huwei

    2014-05-20

    Online coupling of in-tube solid phase microextraction (IT-SPME) with direct analysis in real time mass spectrometry (DART-MS) was realized for the first time and applied in the analysis of triazine herbicides in lake water and orange juice. We incorporated single-wall carbon nanotubes (SWNTs) into a polymer monolith containing methacrylic acid (MAA) and ethylene dimethacrylate (EDMA) to form a novel poly(methacrylic acid-co-ethylene dimethacrylate-co-single wall carbon nanotubes) (poly(MAA-EDMA-SWNT)) monolith, which was then used in IT-SPME for enrichment of six triazine herbicides from water samples. With the online combination of IT-SPME with DART-MS, the analytes desorbed from the monolith were directly ionized by DART and transferred into MS for detection, thus rapid determination was achieved. Compared with regular DART-MS method, this online IT-SPME-DART-MS method was more sensitive and reproducible, because of the IT-SPME procedures and the isotope-labeled internal standard used in the experiment. Six triazine herbicides were determined simultaneously using this method with good linearity (R(2) > 0.998). The limit of quantification (signal-to-noise ratio of S/N = 10) of the six herbicides were only 0.06-0.46 ng/mL. The proposed method has been applied to determine triazine herbicides in lake water and orange juice, showing satisfactory recovery (85%-106%) and reproducibility (relative standard deviation of RSD = 3.1%-10.9%).

  19. Near real-time traffic routing

    NASA Technical Reports Server (NTRS)

    Yang, Chaowei (Inventor); Cao, Ying (Inventor); Xie, Jibo (Inventor); Zhou, Bin (Inventor)

    2012-01-01

    A near real-time physical transportation network routing system comprising: a traffic simulation computing grid and a dynamic traffic routing service computing grid. The traffic simulator produces traffic network travel time predictions for a physical transportation network using a traffic simulation model and common input data. The physical transportation network is divided into a multiple sections. Each section has a primary zone and a buffer zone. The traffic simulation computing grid includes multiple of traffic simulation computing nodes. The common input data includes static network characteristics, an origin-destination data table, dynamic traffic information data and historical traffic data. The dynamic traffic routing service computing grid includes multiple dynamic traffic routing computing nodes and generates traffic route(s) using the traffic network travel time predictions.

  20. A Flexible Real-Time Architecture

    SciTech Connect

    WICKSTROM,GREGORY L.

    2000-08-17

    Assuring hard real-time characteristics of I/O associated with embedded software is often a difficult task. Input-Output related statements are often intermixed with the computational code, resulting in I/O timing that is dependent on the execution path and computational load. One way to mitigate this problem is through the use of interrupts. However, the non-determinism that is introduced by interrupt driven I/O may be so difficult to analyze that it is prohibited in some high consequence systems. This paper describes a balanced hardware/software solution to obtain consistent interrupt-free I/O timing, and results in software that is much more amenable to analysis.

  1. Real Time Simulation of Power Grid Disruptions

    SciTech Connect

    Chinthavali, Supriya; Dimitrovski, Aleksandar D; Fernandez, Steven J; Groer, Christopher S; Nutaro, James J; Olama, Mohammed M; Omitaomu, Olufemi A; Shankar, Mallikarjun; Spafford, Kyle L; Vacaliuc, Bogdan

    2012-11-01

    DOE-OE and DOE-SC workshops (Reference 1-3) identified the key power grid problem that requires insight addressable by the next generation of exascale computing is coupling of real-time data streams (1-2 TB per hour) as the streams are ingested to dynamic models. These models would then identify predicted disruptions in time (2-4 seconds) to trigger the smart grid s self healing functions. This project attempted to establish the feasibility of this approach and defined the scientific issues, and demonstrated example solutions to important smart grid simulation problems. These objectives were accomplished by 1) using the existing frequency recorders on the national grid to establish a representative and scalable real-time data stream; 2) invoking ORNL signature identification algorithms; 3) modeling dynamically a representative region of the Eastern interconnect using an institutional cluster, measuring the scalability and computational benchmarks for a national capability; and 4) constructing a prototype simulation for the system s concept of smart grid deployment. The delivered ORNL enduring capability included: 1) data processing and simulation metrics to design a national capability justifying exascale applications; 2) Software and intellectual property built around the example solutions; 3) demonstrated dynamic models to design few second self-healing.

  2. Real-time applications of neural nets

    SciTech Connect

    Spencer, J.E.

    1989-05-01

    Producing, accelerating and colliding very high power, low emittance beams for long periods is a formidable problem in real-time control. As energy has grown exponentially in time so has the complexity of the machines and their control systems. Similar growth rates have occurred in many areas, e.g., improved integrated circuits have been paid for with comparable increases in complexity. However, in this case, reliability, capability and cost have improved due to reduced size, high production and increased integration which allow various kinds of feedback. In contrast, most large complex systems (LCS) are perceived to lack such possibilities because only one copy is made. Neural nets, as a metaphor for LCS, suggest ways to circumvent such limitations. It is argued that they are logically equivalent to multi-loop feedback/forward control of faulty systems. While complimentary to AI, they mesh nicely with characteristics desired for real-time systems. Such issues are considered, examples given and possibilities discussed. 21 refs., 6 figs.

  3. Real-time applications of neural nets

    SciTech Connect

    Spencer, J.E. )

    1989-10-01

    Producing, accelerating and colliding very high power, low emittance beams for long periods is a formidable problem in real-time control. As energy has grown exponentially in time so has the complexity of the machines and their control systems. Similar growth rates have occurred in many areas e.g. improved integrated circuits have been paid for with comparable increases in complexity. However, in this case, reliability, capability and cost have improved due to reduced size, high production and increased integration which allow various kinds of feedback. In contrast, most large complex systems (LCS) are perceived to lack such possibilities because only one copy is made. Neural nets, as a metaphor for LCS, suggest ways to circumvent such limitations. It is argued that they are logically equivalent to multi-loop feedback/forward control of faulty systems. While complimentary to AI, they mesh nicely with characteristics desired for real-time systems. In this paper, such issues are considered, examples given and possibilities discussed.

  4. Real-time PCR in microfluidic devices

    NASA Astrophysics Data System (ADS)

    Becker, Holger; Hlawatsch, Nadine; Klemm, Richard; Moche, Christian; Hansen-Hagge, Thomas; Gärtner, Claudia

    2014-03-01

    A central method in a standard biochemical laboratory is represented by the polymerase chain reaction (PCR), therefore many attempts have been performed so far to implement this technique in lab-on-a-chip (LOC) devices. PCR is an ideal candidate for miniaturization because of a reduction of assay time and decreased costs for expensive bio-chemicals. In case of the "classical" PCR, detection is done by identification of DNA fragments electrophoretically separated in agarose gels. This method is meanwhile frequently replaced by the so-called Real-Time-PCR because here the exponential increase of amplificates can be observed directly by measurement of DNA interacting fluorescent dyes. Two main methods for on-chip PCRs are available: traditional "batch" PCR in chambers on a chip using thermal cycling, requiring about 30 minutes for a typical PCR protocol and continuous-flow PCR, where the liquid is guided over stationary temperature zones. In the latter case, the PCR protocol can be as fast as 5 minutes. In the presented work, a proof of concept is demonstrated for a real-time-detection of PCR products in microfluidic systems.

  5. Systems Analyze Water Quality in Real Time

    NASA Technical Reports Server (NTRS)

    2010-01-01

    A water analyzer developed under Small Business Innovation Research (SBIR) contracts with Kennedy Space Center now monitors treatment processes at water and wastewater facilities around the world. Originally designed to provide real-time detection of nutrient levels in hydroponic solutions for growing plants in space, the ChemScan analyzer, produced by ASA Analytics Inc., of Waukesha, Wisconsin, utilizes spectrometry and chemometric algorithms to automatically analyze multiple parameters in the water treatment process with little need for maintenance, calibration, or operator intervention. The company has experienced a compound annual growth rate of 40 percent over its 15-year history as a direct result of the technology's success.

  6. Real-Time Ada Demonstration Project

    DTIC Science & Technology

    1989-05-31

    CENER OR OFTAREENGINEERING ADVANCED SOFTWARE TECHNOLOGY Subject: Final Report - Real-Time Ada Demonstration Proj e-t- --.-. SEP 0 1989 D SEA)~ CIN...C02 0921I 6))00 I 31 MAY 1989 *:i ’C O~ 0"ed ~ 842 190 ?’ 45 DEMONSTRATION PROJECT FINAL REPORT PREPARED FOR: U.S. Army HQ CECOM Center for Software ...Engineering Advanced Software Technology Fort Monmouth, NJ 07703-5000 Accession For NTIS G1A&I DTIC TAB PREPARED BY: unannou:1r2d E LabTek Corporation

  7. Real-time teleteaching in medical physics.

    PubMed

    Woo, M; Ng, Kh

    2008-01-01

    Medical physics is a relatively small professional community, usually with a scarcity of expertise that could greatly benefit students entering the field. However, the reach of the profession can span great geographical distances, making the training of students a difficult task. In addition to the requirement of training new students, the evolving field of medical physics, with its many emerging advanced techniques and technologies, could benefit greatly from ongoing continuing education as well as consultation with experts.Many continuing education courses and workshops are constantly being offered, including many web-based study courses and virtual libraries. However, one mode of education and communication that has not been widely used is the real-time interactive process. Video-based conferencing systems do exist, but these usually require a substantial amount of effort and cost to set up.The authors have been working on promoting the ever-expanding capability of the Internet to facilitate the education of medical physics to students entering the field. A pilot project has been carried out for six years and reported previously. The project is a collaboration between the Department of Medical Physics at the Toronto Odette Cancer Centre in Canada and the Department of Biomedical Imaging at the University of Malaya in Malaysia. Since 2001, medical physics graduate students at the University of Malaya have been taught by lecturers from Toronto every year, using the Internet as the main tool of communication.The pilot study explored the different methods that can be used to provide real-time interactive remote education, and delivered traditional classroom lectures as well as hands-on workshops.Another similar project was started in 2007 to offer real-time teaching to a class of medical physics students at Wuhan University in Hubei, China. There are new challenges as well as new opportunities associated with this project. By building an inventory of tools and

  8. Real-time failure control (SAFD)

    NASA Technical Reports Server (NTRS)

    Panossian, Hagop V.; Kemp, Victoria R.; Eckerling, Sherry J.

    1990-01-01

    The Real Time Failure Control program involves development of a failure detection algorithm, referred as System for Failure and Anomaly Detection (SAFD), for the Space Shuttle Main Engine (SSME). This failure detection approach is signal-based and it entails monitoring SSME measurement signals based on predetermined and computed mean values and standard deviations. Twenty four engine measurements are included in the algorithm and provisions are made to add more parameters if needed. Six major sections of research are presented: (1) SAFD algorithm development; (2) SAFD simulations; (3) Digital Transient Model failure simulation; (4) closed-loop simulation; (5) SAFD current limitations; and (6) enhancements planned for.

  9. Low cost real time interactive analysis system

    NASA Technical Reports Server (NTRS)

    Stetina, F.

    1988-01-01

    Efforts continue to develop a low cost real time interactive analysis system for the reception of satellite data. A multi-purpose ingest hardware software frame formatter was demonstrated for GOES and TIROS data and work is proceeding on extending the capability to receive GMS data. A similar system was proposed as an archival and analysis system for use with INSAT data and studies are underway to modify the system to receive the planned SeaWiFS (ocean color) data. This system was proposed as the core of a number of international programs in support of U.S. AID activities. Systems delivered or nearing final testing are listed.

  10. Real-Time Surface Traffic Adviser

    NASA Technical Reports Server (NTRS)

    Glass, Brian J. (Inventor); Spirkovska, Liljana (Inventor); McDermott, William J. (Inventor); Reisman, Ronald J. (Inventor); Gibson, James (Inventor); Iverson, David L. (Inventor)

    2001-01-01

    A real-time data management system which uses data generated at different rates by multiple heterogeneous incompatible data sources are presented. In one embodiment, the invention is as an airport surface traffic data management system (traffic adviser) that electronically interconnects air traffic control, airline, and airport operations user communities to facilitate information sharing and improve taxi queuing. The system uses an expert system to fuse dam from a variety of airline, airport operations, ramp control, and air traffic control sources, in order to establish, predict, and update reference data values for every aircraft surface operation.

  11. Real-Time Reed-Solomon Decoder

    NASA Technical Reports Server (NTRS)

    Maki, Gary K.; Cameron, Kelly B.; Owsley, Patrick A.

    1994-01-01

    Generic Reed-Solomon decoder fast enough to correct errors in real time in practical applications designed to be implemented in fewer and smaller very-large-scale integrated, VLSI, circuit chips. Configured to operate in pipelined manner. One outstanding aspect of decoder design is that Euclid multiplier and divider modules contain Galoisfield multipliers configured as combinational-logic cells. Operates at speeds greater than older multipliers. Cellular configuration highly regular and requires little interconnection area, making it ideal for implementation in extraordinarily dense VLSI circuitry. Flight electronics single chip version of this technology implemented and available.

  12. Terrestrial Real-Time Volcano Monitoring

    NASA Astrophysics Data System (ADS)

    Franke, M.

    2013-12-01

    As volcano monitoring involves more and different sensors from seismic to GPS receivers, from video and thermal cameras to multi-parameter probes measuring temperature, ph values and humidity in the ground and the air, it becomes important to design real-time networks that integrate and leverage the multitude of available parameters. In order to do so some simple principles need to be observed: a) a common time base for all measurements, b) a packetized general data communication protocol for acquisition and distribution, c) an open and well documented interface to the data permitting standard and emerging innovative processing, and d) an intuitive visualization platform for scientists and civil defense personnel. Although mentioned as simple principles, the list above does not necessarily lead to obvious solutions or integrated systems, which is, however, required to take advantage of the available data. Only once the different data streams are put into context to each other in terms of time and location can a broader view be obtained and additional information extracted. The presentation is a summary of currently available technologies and how they can achieve the goal of an integrated real-time volcano monitoring system. A common time base are standard for seismic and GPS networks. In different projects we extended this to video feeds and time-lapse photography. Other probes have been integrated with vault interface enclosures (VIE) as used in the Transportable Array (TA) of the USArray. The VIE can accommodate the sensors employed in volcano monitoring. The TA has shown that Antelope is a versatile and robust middleware. It provides the required packetized general communication protocol that is independent from the actual physical communication link leaving the network design to adopt appropriate and possible hybrid solutions. This applies for the data acquisition and the data/information dissemination providing both a much needed collaboration platform, as

  13. Reconfigurable real-time distributed processing network

    NASA Astrophysics Data System (ADS)

    Page, S. F.; Seely, R. D.; Hickman, D.

    2011-06-01

    This paper describes a novel real-time image and signal processing network, RONINTM, which facilitates the rapid design and deployment of systems providing advanced geospatial surveillance and situational awareness capability. RONINTM is a distributed software architecture consisting of multiple agents or nodes, which can be configured to implement a variety of state-of-the-art computer vision and signal processing algorithms. The nodes operate in an asynchronous fashion and can run on a variety of hardware platforms, thus providing a great deal of scalability and flexibility. Complex algorithmic configuration chains can be assembled using an intuitive graphical interface in a plug-and- play manner. RONINTM has been successfully exploited for a number of applications, ranging from remote event detection to complex multiple-camera real-time 3D object reconstruction. This paper describes the motivation behind the creation of the network, the core design features, and presents details of an example application. Finally, the on-going development of the network is discussed, which is focussed on dynamic network reconfiguration. This allows to the network to automatically adapt itself to node or communications failure by intelligently re-routing network communications and through adaptive resource management.

  14. REal-time COsmic Ray Database (RECORD)

    NASA Astrophysics Data System (ADS)

    Usoskin, I.; Kozlov, Valery; Ksenofontov, Leonid, Kudela, Karel; Starodubtsev, Sergei; Turpanov, Alexey; Yanke, Victor

    2003-07-01

    In this paper we present a first distributed REal-time COsmic Ray Database (RECORD). The aim of the project is to develop a unified database with data from different neutron monitors collected together, in unified format and to provide a user with several commonly used data access methods. The database contains not only original cosmic ray data but also auxiliary data necessary for scientific data analysis. Currently the database includes Lomn.Stit, Moscow, Oulu; Tixie Bay, Yakutsk stations. The main database server is located in IKFIA SB RAS (Yakutsk) but there will be several mirrors of the database. The database and all its mirrors are up dated on the nearly real-time (1 hour) basis. The data access software includes WWW-interface, Perl scripts and C library, which may be linked to a user program. Most of frequently used functions are implemented to make it operable to users without SQL language knowledge. A draft of the data representation standard is suggested, based on common practice of neutron monitor community. The database engine is freely distributed open-sourced PostgreSQL server coupled with a set of replication to ols developed at Bio engineering division of the IRCCS E.Medea, Italy.

  15. Real-time animation of complex hairstyles.

    PubMed

    Volino, Pascal; Magnenat-Thalmann, Nadia

    2006-01-01

    True real-time animation of complex hairstyles on animated characters is the goal of this work, and the challenge is to build a mechanical model of the hairstyle which is sufficiently fast for real-time performance while preserving the particular behavior of the hair medium and maintaining sufficient versatility for simulating any kind of complex hairstyles. Rather than building a complex mechanical model directly related to the structure of the hair strands, we take advantage of a volume free-form deformation scheme. We detail the construction of an efficient lattice mechanical deformation model which represents the volume behavior of the hair strands. The lattice is deformed as a particle system using state-of-the-art numerical methods, and animates the hairs using quadratic B-Spline interpolation. The hairstyle reacts to the body skin through collisions with a metaball-based approximation. The model is highly scalable and allows hairstyles of any complexity to be simulated in any rendering context with the appropriate trade off between accuracy and computation speed, fitting the need of Level-of-Detail optimization schemes.

  16. An efficient real time superresolution ASIC system

    NASA Astrophysics Data System (ADS)

    Reddy, Dikpal; Yue, Zhanfeng; Topiwala, Pankaj

    2008-04-01

    Superresolution of images is an important step in many applications like target recognition where the input images are often grainy and of low quality due to bandwidth constraints. In this paper, we present a real-time superresolution application implemented in ASIC/FPGA hardware, and capable of 30 fps of superresolution by 16X in total pixels. Consecutive frames from the video sequence are grouped and the registered values between them are used to fill the pixels in the higher resolution image. The registration between consecutive frames is evaluated using the algorithm proposed by Schaum et al. The pixels are filled by averaging a fixed number of frames associated with the smallest error distances. The number of frames (the number of nearest neighbors) is a user defined parameter whereas the weights in the averaging process are decided by inverting the corresponding smallest error distances. Wiener filter is used to post process the image. Different input parameters, such as size of input image, enlarging factor and the number of nearest neighbors, can be tuned conveniently by the user. We use a maximum word size of 32 bits to implement the algorithm in Matlab Simulink as well as the hardware, which gives us a fine balance between the number of bits and performance. The algorithm performs with real time speed with very impressive superresolution results.

  17. Real-time Raman sensing without spectrometer

    NASA Astrophysics Data System (ADS)

    Kim, Min Ju; Kim, Sungho; Yang, Timothy K.; Kumar, Dinesh; Bae, Sung Chul

    2015-03-01

    Raman spectroscopy has been a powerful tool in various fields of science and technology ranging from analytical chemistry to biomedical imaging. In spite of unique features, Raman spectroscopy has also some limitations. Among them are weak Raman signal compared to strong fluorescence and relatively complicated setup with expensive and bulky spectrometer. In order to increase the sensitivity of Raman technique, many clever attempts have been made and some of them were very successful including CARS, SRS, and so on. However, these still requires expensive and more complicated setup. In this work, we have attempted to build a real-time compact Raman sensor without spectrometer. Conventional spectrometer was replaced with a narrow-band optical filter and alternatively modulated two lasers with slightly different wavelengths. At one laser, Raman signal from a target molecule was transmitted through the optical filter. At the other laser, this signal was blocked by the optical filter and could not be detected by photon detector. The alternative modulation of two lasers will modulate the Raman signal from a target molecule at the same modulation frequency. This modulated weak Raman signal was amplified by a lock-in amplifier. The advantages of this setup include compactness, low cost, real-time monitoring, and so on. We have tested the sensitivity of this setup and we found that it doesn't have enough sensitivity to detect single molecule-level, but it is still good enough to monitor the change of major chemical composition in the sample.

  18. Real-time sensor data validation

    NASA Technical Reports Server (NTRS)

    Bickmore, Timothy W.

    1994-01-01

    This report describes the status of an on-going effort to develop software capable of detecting sensor failures on rocket engines in real time. This software could be used in a rocket engine controller to prevent the erroneous shutdown of an engine due to sensor failures which would otherwise be interpreted as engine failures by the control software. The approach taken combines analytical redundancy with Bayesian belief networks to provide a solution which has well defined real-time characteristics and well-defined error rates. Analytical redundancy is a technique in which a sensor's value is predicted by using values from other sensors and known or empirically derived mathematical relations. A set of sensors and a set of relations among them form a network of cross-checks which can be used to periodically validate all of the sensors in the network. Bayesian belief networks provide a method of determining if each of the sensors in the network is valid, given the results of the cross-checks. This approach has been successfully demonstrated on the Technology Test Bed Engine at the NASA Marshall Space Flight Center. Current efforts are focused on extending the system to provide a validation capability for 100 sensors on the Space Shuttle Main Engine.

  19. Real-Time and Near Real-Time Data for Space Weather Applications and Services

    NASA Astrophysics Data System (ADS)

    Singer, H. J.; Balch, C. C.; Biesecker, D. A.; Matsuo, T.; Onsager, T. G.

    2015-12-01

    Space weather can be defined as conditions in the vicinity of Earth and in the interplanetary environment that are caused primarily by solar processes and influenced by conditions on Earth and its atmosphere. Examples of space weather are the conditions that result from geomagnetic storms, solar particle events, and bursts of intense solar flare radiation. These conditions can have impacts on modern-day technologies such as GPS or electric power grids and on human activities such as astronauts living on the International Space Station or explorers traveling to the moon or Mars. While the ultimate space weather goal is accurate prediction of future space weather conditions, for many applications and services, we rely on real-time and near-real time observations and model results for the specification of current conditions. In this presentation, we will describe the space weather system and the need for real-time and near-real time data that drive the system, characterize conditions in the space environment, and are used by models for assimilation and validation. Currently available data will be assessed and a vision for future needs will be given. The challenges for establishing real-time data requirements, as well as acquiring, processing, and disseminating the data will be described, including national and international collaborations. In addition to describing how the data are used for official government products, we will also give examples of how these data are used by both the public and private sector for new applications that serve the public.

  20. Real-time forecasts of dengue epidemics

    NASA Astrophysics Data System (ADS)

    Yamana, T. K.; Shaman, J. L.

    2015-12-01

    Dengue is a mosquito-borne viral disease prevalent in the tropics and subtropics, with an estimated 2.5 billion people at risk of transmission. In many areas with endemic dengue, disease transmission is seasonal but prone to high inter-annual variability with occasional severe epidemics. Predicting and preparing for periods of higher than average transmission is a significant public health challenge. Here we present a model of dengue transmission and a framework for optimizing model simulations with real-time observational data of dengue cases and environmental variables in order to generate ensemble-based forecasts of the timing and severity of disease outbreaks. The model-inference system is validated using synthetic data and dengue outbreak records. Retrospective forecasts are generated for a number of locations and the accuracy of these forecasts is quantified.

  1. Identifying financial crises in real time

    NASA Astrophysics Data System (ADS)

    da Fonseca, Eder Lucio; Ferreira, Fernando F.; Muruganandam, Paulsamy; Cerdeira, Hilda A.

    2013-03-01

    Following the thermodynamic formulation of a multifractal measure that was shown to enable the detection of large fluctuations at an early stage, here we propose a new index which permits us to distinguish events like financial crises in real time. We calculate the partition function from which we can obtain thermodynamic quantities analogous to the free energy and specific heat. The index is defined as the normalized energy variation and it can be used to study the behavior of stochastic time series, such as financial market daily data. Famous financial market crashes-Black Thursday (1929), Black Monday (1987) and the subprime crisis (2008)-are identified with clear and robust results. The method is also applied to the market fluctuations of 2011. From these results it appears as if the apparent crisis of 2011 is of a different nature to the other three. We also show that the analysis has forecasting capabilities.

  2. Near Real Time Quantitative Gas Analysis Techniques

    NASA Astrophysics Data System (ADS)

    Herget, William F.; Tromp, Marianne L.; Anderson, Charles R.

    1985-12-01

    A Fourier transform infrared (FT-IR) - based system has been developed and is undergoing evaluation for near real time multicomponent quantitative analysis of undiluted gaseous automotive exhaust emissions. The total system includes: (1) a gas conditioning system (GCS) for tracer gas injection, gas mixing, and temperature stabilization; and (2) an exhaust gas analyzer (EGA) consisting of a sample cell, an FT-IR system, and a computerized data processing system. Tests have shown that the system can monitor about 20 individual species (concentrations down to the 1-20 ppm range) with a time resolution of one second. Tests have been conducted on a chassis dynamometer system utilizing different autos, different fuels, and different driving cycles. Results were compared with those obtained using a standard constant volume sampling (CVS) system.

  3. Towards real time speckle controlled retinal photocoagulation

    NASA Astrophysics Data System (ADS)

    Bliedtner, Katharina; Seifert, Eric; Stockmann, Leoni; Effe, Lisa; Brinkmann, Ralf

    2016-03-01

    Photocoagulation is a laser treatment widely used for the therapy of several retinal diseases. Intra- and inter-individual variations of the ocular transmission, light scattering and the retinal absorption makes it impossible to achieve a uniform effective exposure and hence a uniform damage throughout the therapy. A real-time monitoring and control of the induced damage is highly requested. Here, an approach to realize a real time optical feedback using dynamic speckle analysis is presented. A 532 nm continuous wave Nd:YAG laser is used for coagulation. During coagulation, speckle dynamics are monitored by a coherent object illumination using a 633nm HeNe laser and analyzed by a CMOS camera with a frame rate up to 1 kHz. It is obvious that a control system needs to determine whether the desired damage is achieved to shut down the system in a fraction of the exposure time. Here we use a fast and simple adaption of the generalized difference algorithm to analyze the speckle movements. This algorithm runs on a FPGA and is able to calculate a feedback value which is correlated to the thermal and coagulation induced tissue motion and thus the achieved damage. For different spot sizes (50-200 μm) and different exposure times (50-500 ms) the algorithm shows the ability to discriminate between different categories of retinal pigment epithelial damage ex-vivo in enucleated porcine eyes. Furthermore in-vivo experiments in rabbits show the ability of the system to determine tissue changes in living tissue during coagulation.

  4. Real-time gas and particle-phase organic acids measurement at a forest site using chemical ionization high-resolution time-of-flight mass spectrometry during BEACHON-RoMBAS

    NASA Astrophysics Data System (ADS)

    Yatavelli, L. R.; Stark, H.; Kimmel, J.; Cubison, M.; Day, D. A.; Jayne, J.; Thornton, J. A.; Worsnop, D. R.; Jimenez, J. L.

    2011-12-01

    We present measurement of organic acids in gas and aerosol particles conducted in a ponderosa pine forest during July and August 2011 as part of the Bio-hydro-atmosphere interactions of Energy, Aerosols, Carbon, H2O, Organics & Nitrogen - Rocky Mountain Biogenic Aerosol Study (BEACHON-RoMBAS; http://tinyurl.com/BEACHON-RoMBAS). The measurement technique is based on chemical ionization, high-resolution time-of-flight mass spectrometry and utilizes a Micro-Orifice Volatilization Impactor [MOVI-CI-HR-ToFMS; Yatavelli et al., AS&T, 2010] to collect sub-micron aerosol particles while simultaneously measuring the gas-phase composition. The collected particles are subsequently analyzed by temperature-programmed thermal desorption. The reagent ion chosen for this campaign is the acetate anion (CH3C(O)O-, m/z 59), which reacts selectively via proton transfer with compounds that are stronger gas-phase acids than acetic acid [Veres et al., IJMS, 2008]. Preliminary results show substantial particle-phase concentrations of biogenic oxidation products such as hydroxy-glutaric acid, pinic acid, pinonic acid, and hydroxy-pinonic acid along with numerous lower and higher molecular weight organic acids. Correlations of the organic acid concentrations with meteorological, gas and aerosol parameters measured by other instrumentation are investigated in order to understand the formation, transformation, and partitioning of gas and particle-phase organic acids in a forested environment dominated by terpenes.

  5. Single photon ionization (SPI) via incoherent VUV-excimer light: robust and compact time-of-flight mass spectrometer for on-line, real-time process gas analysis.

    PubMed

    Mühlberger, F; Wieser, J; Ulrich, A; Zimmermann, R

    2002-08-01

    Fast on-line detection of organic compounds from complex mixtures, such as industrial process gas streams, require selective and sensitive analytical methods. One feasible approach for this purpose is the use of mass spectrometry (MS) with a selective and soft (fragment-free) ionization technique, such as chemical ionization (CI) or photo ionization (PI). Single photon ionization (SPI) with vacuum ultraviolet (VUV) light is a particularly sof tionization technique, well-suited for detection of both aromatic and aliphatic species. Problematic, however, is the generation of the VUV light. In general, the vacuum ultraviolet (VUV) light sources for SPI-MS are based either on lasers (e.g., 118-nm radiation generated by frequency-tripling of the third harmonic of a Nd:YAG laser) or on conventional VUV lamps, such as deuterium lamps. Althoughthe laser-based techniques are very sophisticated and expensive, the conventional lamps have serious drawbacks regarding their optical parameters, such as low-output power, low spectral power density, and broad emission bands. In this work, a novel excimer VUV light source, in which an electron beam is used to form rare gas excimer species, is used. The excimer VUV light sourceproduces brilliant and intense VUV light. The novel VUV light source was coupled to a compact and mobile time-of-flight mass spectrometer (TOFMS). A special interface design, including optical (VUV optics) as well as electronic measures (e.g., pulsed ion extraction) was realized. The use of the excimer VUV lamp for SPI will allow the realization of very compact, rugged, and sensitive SPI-TOFMS devices, which preferably will be adapted for process analytical application or monitoring issues (e.g., chemical warfare detection). The excimer VUV-lamp technology delivers VUV light with a good beam quality and high-output power at low costs. Furthermore, it allows changing the emitted wavelength as well as the bandwidth of the excimer VUV lamp in t he 100-200-nm region

  6. Real-time visualization of joint cavitation.

    PubMed

    Kawchuk, Gregory N; Fryer, Jerome; Jaremko, Jacob L; Zeng, Hongbo; Rowe, Lindsay; Thompson, Richard

    2015-01-01

    Cracking sounds emitted from human synovial joints have been attributed historically to the sudden collapse of a cavitation bubble formed as articular surfaces are separated. Unfortunately, bubble collapse as the source of joint cracking is inconsistent with many physical phenomena that define the joint cracking phenomenon. Here we present direct evidence from real-time magnetic resonance imaging that the mechanism of joint cracking is related to cavity formation rather than bubble collapse. In this study, ten metacarpophalangeal joints were studied by inserting the finger of interest into a flexible tube tightened around a length of cable used to provide long-axis traction. Before and after traction, static 3D T1-weighted magnetic resonance images were acquired. During traction, rapid cine magnetic resonance images were obtained from the joint midline at a rate of 3.2 frames per second until the cracking event occurred. As traction forces increased, real-time cine magnetic resonance imaging demonstrated rapid cavity inception at the time of joint separation and sound production after which the resulting cavity remained visible. Our results offer direct experimental evidence that joint cracking is associated with cavity inception rather than collapse of a pre-existing bubble. These observations are consistent with tribonucleation, a known process where opposing surfaces resist separation until a critical point where they then separate rapidly creating sustained gas cavities. Observed previously in vitro, this is the first in-vivo macroscopic demonstration of tribonucleation and as such, provides a new theoretical framework to investigate health outcomes associated with joint cracking.

  7. A real-time prediction of UTC

    NASA Astrophysics Data System (ADS)

    Thomas, Claudine; Allan, David W.

    1994-05-01

    The reference time scale for all scientific and technologic applications on the Earth, the Universal Coordinated Time (UTC), must be as stable, reliable, and accurate as possible. With this in view the BIPM and before it the BIH, have always calculated and then disseminated UTC with a delay of about 80 days. There are three fundamental reasons for doing this: (1) It takes some weeks for data, gathered from some 200 clocks spread world-wide, to be collected and for errors to be eliminated; (2) changes in clock rates can only be measured with high precision well after the fact; and (3) the measurement noise originating in time links, in particular using Loran-C, is smoothed out only when averaging over an extended period. Until mid-1992, the ultimate stability of UTC was reached at averaging times of about 100 days and corresponded to an Allan deviation sigma(sub y)(tau) of about 1,5x10(exp -14) then compared to the best primary clock in the world, the PTB CS2. For several years now, a predicted UTC has been computed by the USNO through an extrapolation of the values as published in deferred time by the BIPM. This is made available through the USNO Series 4, through the USNO Automated Data Service, and through GPS signals. Due to the instability of UTC, the poor predictability of the available clocks, and the intentional SA degradation of GPS signals, the real-time access to this extrapolated UTC has represented the true deferred-time UTC only to within several hundreds of nanoseconds.

  8. A real-time prediction of UTC

    NASA Technical Reports Server (NTRS)

    Thomas, Claudine; Allan, David W.

    1994-01-01

    The reference time scale for all scientific and technologic applications on the Earth, the Universal Coordinated Time (UTC), must be as stable, reliable, and accurate as possible. With this in view the BIPM and before it the BIH, have always calculated and then disseminated UTC with a delay of about 80 days. There are three fundamental reasons for doing this: (1) It takes some weeks for data, gathered from some 200 clocks spread world-wide, to be collected and for errors to be eliminated; (2) changes in clock rates can only be measured with high precision well after the fact; and (3) the measurement noise originating in time links, in particular using Loran-C, is smoothed out only when averaging over an extended period. Until mid-1992, the ultimate stability of UTC was reached at averaging times of about 100 days and corresponded to an Allan deviation sigma(sub y)(tau) of about 1,5x10(exp -14) then compared to the best primary clock in the world, the PTB CS2. For several years now, a predicted UTC has been computed by the USNO through an extrapolation of the values as published in deferred time by the BIPM. This is made available through the USNO Series 4, through the USNO Automated Data Service, and through GPS signals. Due to the instability of UTC, the poor predictability of the available clocks, and the intentional SA degradation of GPS signals, the real-time access to this extrapolated UTC has represented the true deferred-time UTC only to within several hundreds of nanoseconds.

  9. Real-time value-driven diagnosis

    NASA Technical Reports Server (NTRS)

    Dambrosio, Bruce

    1995-01-01

    Diagnosis is often thought of as an isolated task in theoretical reasoning (reasoning with the goal of updating our beliefs about the world). We present a decision-theoretic interpretation of diagnosis as a task in practical reasoning (reasoning with the goal of acting in the world), and sketch components of our approach to this task. These components include an abstract problem description, a decision-theoretic model of the basic task, a set of inference methods suitable for evaluating the decision representation in real-time, and a control architecture to provide the needed continuing coordination between the agent and its environment. A principal contribution of this work is the representation and inference methods we have developed, which extend previously available probabilistic inference methods and narrow, somewhat, the gap between probabilistic and logical models of diagnosis.

  10. Real time speech formant analyzer and display

    DOEpatents

    Holland, George E.; Struve, Walter S.; Homer, John F.

    1987-01-01

    A speech analyzer for interpretation of sound includes a sound input which converts the sound into a signal representing the sound. The signal is passed through a plurality of frequency pass filters to derive a plurality of frequency formants. These formants are converted to voltage signals by frequency-to-voltage converters and then are prepared for visual display in continuous real time. Parameters from the inputted sound are also derived and displayed. The display may then be interpreted by the user. The preferred embodiment includes a microprocessor which is interfaced with a television set for displaying of the sound formants. The microprocessor software enables the sound analyzer to present a variety of display modes for interpretive and therapeutic used by the user.

  11. REAL TIME DATA FOR REMEDIATION ACTIVITIES [11505

    SciTech Connect

    BROCK CT

    2011-01-13

    Health physicists from the CH2M HILL Plateau Remediation Company collaborated with Berkeley Nucleonics Corporation to modify the SAM 940 isotope identifier instrument to be used for nuclear waste remediation. These modifications coupled with existing capabilities of the SAM 940 have proven to be invaluable during remediation activities, reducing disposal costs by allowing swift remediation of targeted areas that have been identified as having isotopes of concern (IOC), and eliminating multiple visits to sites by declaring an excavation site clear of IOCs before demobilizing from the site. These advantages are enabled by accumulating spectral data for specific isotopes that is nearly 100 percent free of false positives, which are filtered out in 'real time.'

  12. Cerebral Autoregulation Real-Time Monitoring

    PubMed Central

    Tsalach, Adi; Ratner, Eliahu; Lokshin, Stas; Silman, Zmira; Breskin, Ilan; Budin, Nahum; Kamar, Moshe

    2016-01-01

    Cerebral autoregulation is a mechanism which maintains constant cerebral blood flow (CBF) despite changes in mean arterial pressure (MAP). Assessing whether this mechanism is intact or impaired and determining its boundaries is important in many clinical settings, where primary or secondary injuries to the brain may occur. Herein we describe the development of a new ultrasound tagged near infra red light monitor which tracks CBF trends, in parallel, it continuously measures blood pressure and correlates them to produce a real time autoregulation index. Its performance is validated in both in-vitro experiment and a pre-clinical case study. Results suggest that using such a tool, autoregulation boundaries as well as its impairment or functioning can be identified and assessed. It may therefore assist in individualized MAP management to ensure adequate organ perfusion and reduce the risk of postoperative complications, and might play an important role in patient care. PMID:27571474

  13. Real-time, face recognition technology

    SciTech Connect

    Brady, S.

    1995-11-01

    The Institute for Scientific Computing Research (ISCR) at Lawrence Livermore National Laboratory recently developed the real-time, face recognition technology KEN. KEN uses novel imaging devices such as silicon retinas developed at Caltech or off-the-shelf CCD cameras to acquire images of a face and to compare them to a database of known faces in a robust fashion. The KEN-Online project makes that recognition technology accessible through the World Wide Web (WWW), an internet service that has recently seen explosive growth. A WWW client can submit face images, add them to the database of known faces and submit other pictures that the system tries to recognize. KEN-Online serves to evaluate the recognition technology and grow a large face database. KEN-Online includes the use of public domain tools such as mSQL for its name-database and perl scripts to assist the uploading of images.

  14. Optimal, real-time control--colliders

    SciTech Connect

    Spencer, J.E.

    1991-05-01

    With reasonable definitions, optimal control is possible for both classical and quantal systems with new approaches called PISC(Parallel) and NISC(Neural) from analogy with RISC (Reduced Instruction Set Computing). If control equals interaction, observation and comparison to some figure of merit with interaction via external fields, then optimization comes from varying these fields to give design or operating goals. Structural stability can then give us tolerance and design constraints. But simulations use simplified models, are not in real-time and assume fixed or stationary conditions, so optimal control goes far beyond convergence rates of algorithms. It is inseparable from design and this has many implications for colliders. 12 refs., 3 figs.

  15. Real-time color holographic interferometry

    NASA Astrophysics Data System (ADS)

    Desse, Jean-Michel; Albe, Felix; Tribillon, Jean-Louis

    2002-09-01

    A new optical technique based on real-time color holographic interferometry has been developed for analyzing unsteady aerodynamic wakes in fluid mechanics or for measuring displacements and deformations in solid mechanics. The technique's feasibility is demonstrated here. It uses three coherent wavelengths produced simultaneously by a cw laser (mixed argon and krypton). Holograms are recorded on single-layer panchromatic silver halide (Slavich PFG 03C) plates. Results show the optical setup can be adjusted to obtain a uniform background color. The interference fringe pattern visualized is large and colored and exhibits a single central white fringe, which makes the zero order of the interferogram easy to identify. An application in a subsonic wind tunnel is presented, in which the unsteady wake past a cylinder is recorded at high rate.

  16. Real-time color holographic interferometry.

    PubMed

    Desse, Jean-Michel; Albe, Félix; Tribillon, Jean-Louis

    2002-09-01

    A new optical technique based on real-time color holographic interferometry has been developed for analyzing unsteady aerodynamic wakes in fluid mechanics or for measuring displacements and deformations in solid mechanics. The technique's feasibility is demonstrated here. It uses three coherent wavelengths produced simultaneously by a cw laser (mixed argon and krypton). Holograms are recorded on single-layer panchromatic silver halide (Slavich PFG 03C) plates. Results show the optical setup can be adjusted to obtain a uniform background color. The interference fringe pattern visualized is large and colored and exhibits a single central white fringe, which makes the zero order of the interferogram easy to identify. An application in a subsonic wind tunnel is presented, in which the unsteady wake past a cylinder is recorded at high rate.

  17. Real time speech formant analyzer and display

    DOEpatents

    Holland, G.E.; Struve, W.S.; Homer, J.F.

    1987-02-03

    A speech analyzer for interpretation of sound includes a sound input which converts the sound into a signal representing the sound. The signal is passed through a plurality of frequency pass filters to derive a plurality of frequency formants. These formants are converted to voltage signals by frequency-to-voltage converters and then are prepared for visual display in continuous real time. Parameters from the inputted sound are also derived and displayed. The display may then be interpreted by the user. The preferred embodiment includes a microprocessor which is interfaced with a television set for displaying of the sound formants. The microprocessor software enables the sound analyzer to present a variety of display modes for interpretive and therapeutic used by the user. 19 figs.

  18. A Measure of Real-Time Intelligence

    NASA Astrophysics Data System (ADS)

    Gavane, Vaibhav

    2013-03-01

    We propose a new measure of intelligence for general reinforcement learning agents, based on the notion that an agent's environment can change at any step of execution of the agent. That is, an agent is considered to be interacting with its environment in real-time. In this sense, the resulting intelligence measure is more general than the universal intelligence measure (Legg and Hutter, 2007) and the anytime universal intelligence test (Hernández-Orallo and Dowe, 2010). A major advantage of the measure is that an agent's computational complexity is factored into the measure in a natural manner. We show that there exist agents with intelligence arbitrarily close to the theoretical maximum, and that the intelligence of agents depends on their parallel processing capability. We thus believe that the measure can provide a better evaluation of agents and guidance for building practical agents with high intelligence.

  19. Near real-time stereo vision system

    NASA Technical Reports Server (NTRS)

    Anderson, Charles H. (Inventor); Matthies, Larry H. (Inventor)

    1993-01-01

    The apparatus for a near real-time stereo vision system for use with a robotic vehicle is described. The system is comprised of two cameras mounted on three-axis rotation platforms, image-processing boards, a CPU, and specialized stereo vision algorithms. Bandpass-filtered image pyramids are computed, stereo matching is performed by least-squares correlation, and confidence ranges are estimated by means of Bayes' theorem. In particular, Laplacian image pyramids are built and disparity maps are produced from the 60 x 64 level of the pyramids at rates of up to 2 seconds per image pair. The first autonomous cross-country robotic traverses (of up to 100 meters) have been achieved using the stereo vision system of the present invention with all computing done onboard the vehicle. The overall approach disclosed herein provides a unifying paradigm for practical domain-independent stereo ranging.

  20. Real-time data flow and product generating for GNSS

    NASA Technical Reports Server (NTRS)

    Muellerschoen, Ronald J.; Caissy, Mark

    2004-01-01

    The last IGS workshop with the theme 'Towards Real-Time' resulted in the design of a prototype for real-time data and sharing within the IGS. A prototype real-time network is being established that will serve as a test bed for real-time activities within the IGS. We review the developments of the prototype and discuss some of the existing methods and related products of real-time GNSS systems. Recommendations are made concerning real-time data distribution and product generation.

  1. A tool for modeling concurrent real-time computation

    NASA Technical Reports Server (NTRS)

    Sharma, D. D.; Huang, Shie-Rei; Bhatt, Rahul; Sridharan, N. S.

    1990-01-01

    Real-time computation is a significant area of research in general, and in AI in particular. The complexity of practical real-time problems demands use of knowledge-based problem solving techniques while satisfying real-time performance constraints. Since the demands of a complex real-time problem cannot be predicted (owing to the dynamic nature of the environment) powerful dynamic resource control techniques are needed to monitor and control the performance. A real-time computation model for a real-time tool, an implementation of the QP-Net simulator on a Symbolics machine, and an implementation on a Butterfly multiprocessor machine are briefly described.

  2. Otolaryngology consultations by real-time telemedicine.

    PubMed Central

    Ullah, R.; Gilliland, D.; Adams, D.

    2002-01-01

    We aimed to assess the value of real-time telemedicine using low cost videoconferencing equipment for otorhinolaryngology consultations. A general practitioner, using low cost videoconferencing equipment, presented patients to an otorhinolaryngologist. After history taking and clinical examination, investigations were requested if required and a diagnosis and management plan formulated. The patients were then seen, by the same otorhinolaryngologist, for a conventional face-to-face consultation. Differences in the history, clinical examination and investigation requests were reported. The accuracy of diagnosis and correlation of management plans between the two consultations were analysed. Forty-three patients were admitted to the study but one, a young child, refused examination either by tele-link or the conventional approach and had to be excluded. There were thus 42 patients with 55 diagnoses included in the trial, 26 (62%) females and 16 (38%) males. Age range was 5 months to 70 years. There was no difficulty with any of the patients in obtaining an accurate history and ordering investigations, if required, via the telelink. Clinical examination during the tele-link consultation was inadequate for eight out of the first 20 patients, resulting in a wrong diagnosis in three patients and a missed diagnosis in five patients. All of the next 22 patients had a correct diagnosis and management plan. Comparison of data from the two types of consultation showed that a correct diagnosis and management plan was made in 34 patients. Low cost real-time telemedicine is a useful technique, providing reliable otorhinolaryngology consultations in a general practice setting. However initial difficulties due to inexperience in using the equipment need to be overcome. PMID:12137160

  3. Public Science with Real-Time Experiments

    NASA Astrophysics Data System (ADS)

    Lenardic, A.

    2013-12-01

    One of the best ways for professional scientists to engage in public outreach is to get outside of the university and/or lab walls and go out into the public. That is, go to public spaces to do some science experiments with the public - this includes students of all ages that constitute that public. Technological advance in portable measurement gear now allow one to do real, or near real, time experiments in outdoor, public spaces. We have been running a meta-experiment of this sort, aimed at the public display of science, for about a year now in Houston TX at the Lee and Joe Jamail Skatepark. The project goes under the title of Sk8Lab Houston and has introduced students of all ages to the power of scientific experimentation. We bring a portable science pack with us to the park. The pack has a range of wireless measurement gear that allow experiments to be done on the spot. Some of the experiments are designed by us but many are designed on by whoever suggests them to us that day. Over time the Sk8Lab scientists have built up a level of "trust" with the people who frequent the park (no one feels like we are gonna grade them at the park and they know that the learning is not on some regimented clock). This has broken down some learning walls and allowed for a more informal mode of exploration and a more genuine mode of experimentation (as compared to what often happens in class labs when students feel like they are just being forced to reproduce some known result). We will describe some of the test case experiments we have run and also discuss some of the trials, tribulations, and happy successes (many unplanned) along the way.

  4. A high sensitivity real-time NVR monitor. [Nonvolatile Residue

    NASA Technical Reports Server (NTRS)

    Bowers, William D.; Chuan, R. L.

    1992-01-01

    The use of a temperature-controlled 200-MHz SAW resonator piezoelectric mass microbalance to monitor the mass of nonvolatile residue (NVR) deposited on its surface in real time is reported. The fundamental frequency of this device is mainly dependent on the configuration of the transducers and not on the thickness of the substrate. Therefore, higher operating frequencies can be achieved without reducing the thickness of the crystal. The real-time instrument was integrated onto a conventional stainless steel NVR plate and operated flawlessly over a 14-d period at Kennedy Space Center and successfully measured less than 1 ng/sq cm d NVR contamination. Contamination episodes detected by the instrument were correlated with scheduled activities on the test stand. Under the assumption of a baseline noise level of +/- 2 Hz, the absolute mass lower limit of detection would be 0.065 ng/sq cm. This would enable the detection of a daily NVR deposition rate of less than 0.1 ng/sq cm d.

  5. Approaching near real-time biosensing: microfluidic microsphere based biosensor for real-time analyte detection.

    PubMed

    Cohen, Noa; Sabhachandani, Pooja; Golberg, Alexander; Konry, Tania

    2015-04-15

    In this study we describe a simple lab-on-a-chip (LOC) biosensor approach utilizing well mixed microfluidic device and a microsphere-based assay capable of performing near real-time diagnostics of clinically relevant analytes such cytokines and antibodies. We were able to overcome the adsorption kinetics reaction rate-limiting mechanism, which is diffusion-controlled in standard immunoassays, by introducing the microsphere-based assay into well-mixed yet simple microfluidic device with turbulent flow profiles in the reaction regions. The integrated microsphere-based LOC device performs dynamic detection of the analyte in minimal amount of biological specimen by continuously sampling micro-liter volumes of sample per minute to detect dynamic changes in target analyte concentration. Furthermore we developed a mathematical model for the well-mixed reaction to describe the near real time detection mechanism observed in the developed LOC method. To demonstrate the specificity and sensitivity of the developed real time monitoring LOC approach, we applied the device for clinically relevant analytes: Tumor Necrosis Factor (TNF)-α cytokine and its clinically used inhibitor, anti-TNF-α antibody. Based on the reported results herein, the developed LOC device provides continuous sensitive and specific near real-time monitoring method for analytes such as cytokines and antibodies, reduces reagent volumes by nearly three orders of magnitude as well as eliminates the washing steps required by standard immunoassays.

  6. Clinical experience with real-time ultrasound

    NASA Astrophysics Data System (ADS)

    Chimiak, William J.; Wolfman, Neil T.; Covitz, Wesley

    1995-05-01

    After testing the extended multimedia interface (EMMI) product which is an asynchronous transmission mode (ATM) user to network interface (UNI) of AT&T at the Society for Computer Applications in Radiology conference in Winston-Salem, the Department of Radiology together with AT&T are implementing a tele-ultrasound system to combine real- time ultrasound with the static imaging features of more traditional digital ultrasound systems. Our current ultrasound system archives digital images to an optical disk system. Static images are sent using our digital radiology systems. This could be transferring images from one digital imaging and communications (DICOM)-compliant machine to another, or the current image transfer methodologies. The prototype of a live ultrasound system using the EMMI demonstrated the feasibility of doing live ultrasound. We now are developing the scenarios using a mix of the two methodologies. Utilizing EMMI technology, radiologists at the BGSM review at a workstation both static images and real-time scanning done by a technologist on patients at a remote site in order to render on-line primary diagnosis. Our goal is to test the feasibility of operating an ultrasound laboratory at a remote site utilizing a trained technologist without the necessity of having a full-time radiologist at that site. Initial plans are for a radiologist to review an initial set of static images on a patient taken by the technologist. If further scanning is required, the EMMI is used to transmit real-time imaging and audio using the audio input of a standard microphone system and the National Television Standards Committee (NTSC) output of the ultrasound equipment from the remote site to the radiologist in the department review station. The EMMI digitally encodes this data and places it in an ATM format. This ATM data stream goes to the GCNS2000 and then to the other EMMI where the ATM data stream is decoded into the live studies and voice communication which are then

  7. Capability of a Mobile Monitoring System to Provide Real-Time Data Broadcasting and Near Real-Time Source Attribution

    NASA Astrophysics Data System (ADS)

    Erickson, M.; Olaguer, J.; Wijesinghe, A.; Colvin, J.; Neish, B.; Williams, J.

    2014-12-01

    It is becoming increasingly important to understand the emissions and health effects of industrial facilities. Many areas have no or limited sustained monitoring capabilities, making it difficult to quantify the major pollution sources affecting human health, especially in fence line communities. Developments in real-time monitoring and micro-scale modeling offer unique ways to tackle these complex issues. This presentation will demonstrate the capability of coupling real-time observations with micro-scale modeling to provide real-time information and near real-time source attribution. The Houston Advanced Research Center constructed the Mobile Acquisition of Real-time Concentrations (MARC) laboratory. MARC consists of a Ford E-350 passenger van outfitted with a Proton Transfer Reaction Mass Spectrometer (PTR-MS) and meteorological equipment. This allows for the fast measurement of various VOCs important to air quality. The data recorded from the van is uploaded to an off-site database and the information is broadcast to a website in real-time. This provides for off-site monitoring of MARC's observations, which allows off-site personnel to provide immediate input to the MARC operators on how to best achieve project objectives. The information stored in the database can also be used to provide near real-time source attribution. An inverse model has been used to ascertain the amount, location, and timing of emissions based on MARC measurements in the vicinity of industrial sites. The inverse model is based on a 3D micro-scale Eulerian forward and adjoint air quality model known as the HARC model. The HARC model uses output from the Quick Urban and Industrial Complex (QUIC) wind model and requires a 3D digital model of the monitored facility based on lidar or industrial permit data. MARC is one of the instrument platforms deployed during the 2014 Benzene and other Toxics Exposure Study (BEE-TEX) in Houston, TX. The main goal of the study is to quantify and explain the

  8. Real-Time Principal-Component Analysis

    NASA Technical Reports Server (NTRS)

    Duong, Vu; Duong, Tuan

    2005-01-01

    A recently written computer program implements dominant-element-based gradient descent and dynamic initial learning rate (DOGEDYN), which was described in Method of Real-Time Principal-Component Analysis (NPO-40034) NASA Tech Briefs, Vol. 29, No. 1 (January 2005), page 59. To recapitulate: DOGEDYN is a method of sequential principal-component analysis (PCA) suitable for such applications as data compression and extraction of features from sets of data. In DOGEDYN, input data are represented as a sequence of vectors acquired at sampling times. The learning algorithm in DOGEDYN involves sequential extraction of principal vectors by means of a gradient descent in which only the dominant element is used at each iteration. Each iteration includes updating of elements of a weight matrix by amounts proportional to a dynamic initial learning rate chosen to increase the rate of convergence by compensating for the energy lost through the previous extraction of principal components. In comparison with a prior method of gradient-descent-based sequential PCA, DOGEDYN involves less computation and offers a greater rate of learning convergence. The sequential DOGEDYN computations require less memory than would parallel computations for the same purpose. The DOGEDYN software can be executed on a personal computer.

  9. Real-time optoacoustic monitoring of stroke

    NASA Astrophysics Data System (ADS)

    Kneipp, Moritz; Turner, Jake; Hambauer, Sebastian; Krieg, Sandro M.; Lehmberg, Jens; Lindauer, Ute; Razansky, Daniel

    2014-03-01

    Characterizing disease progression and identifying possible therapeutic interventions in stroke is greatly aided by the use of longitudinal function imaging studies. In this study, we investigate the applicability of real-time multispectral optoacoustic tomography (MSOT) as a tool for non-invasive monitoring of the progression of stroke in the whole brain. The middle cerebral artery occlusion (MCAO) method was used to induce stroke. Mice were imaged under isoflurane anesthesia preoperatively and at several time points during and after the 60-minute occlusion. The animals were sacrificed after 24 hours and their excised brains frozen at -80°C for sectioning. The cryosection were stained using H&E staining to identify the ischemic lesion. Major vessels are readily identifiable in the whole mouse head in the in vivo optoacoustic scans. During ischemia, a reduction in cerebral blood volume is detectable in the cortex. Post ischemia, spectral unmixing of the optoacoustic signals shows an asymmetry of the deoxygenated hemoglobin in the hemisphere affected by MCAO. This hypoxic area was mainly located around the boundary of the ischemic lesion and was therefore identified as the ischemic penumbra. Non-invasive functional MSOT imaging is able to visualize the hypoxic penumbra in brains affected by stroke. Stopping the spread of the infarct area and revitalizing the penumbra is central in stroke research, this new imaging technique may therefore prove to be a valuable tool in the monitoring and developing new treatments.

  10. Ames Lab 101: Real-Time 3D Imaging

    SciTech Connect

    Zhang, Song

    2010-01-01

    Ames Laboratory scientist Song Zhang explains his real-time 3-D imaging technology. The technique can be used to create high-resolution, real-time, precise, 3-D images for use in healthcare, security, and entertainment applications.

  11. Ames Lab 101: Real-Time 3D Imaging

    ScienceCinema

    Zhang, Song

    2016-07-12

    Ames Laboratory scientist Song Zhang explains his real-time 3-D imaging technology. The technique can be used to create high-resolution, real-time, precise, 3-D images for use in healthcare, security, and entertainment applications.

  12. CRANS - CONFIGURABLE REAL-TIME ANALYSIS SYSTEM

    NASA Technical Reports Server (NTRS)

    Mccluney, K.

    1994-01-01

    In a real-time environment, the results of changes or failures in a complex, interconnected system need evaluation quickly. Tabulations showing the effects of changes and/or failures of a given item in the system are generally only useful for a single input, and only with regard to that item. Subsequent changes become harder to evaluate as combinations of failures produce a cascade effect. When confronted by multiple indicated failures in the system, it becomes necessary to determine a single cause. In this case, failure tables are not very helpful. CRANS, the Configurable Real-time ANalysis System, can interpret a logic tree, constructed by the user, describing a complex system and determine the effects of changes and failures in it. Items in the tree are related to each other by Boolean operators. The user is then able to change the state of these items (ON/OFF FAILED/UNFAILED). The program then evaluates the logic tree based on these changes and determines any resultant changes to other items in the tree. CRANS can also search for a common cause for multiple item failures, and allow the user to explore the logic tree from within the program. A "help" mode and a reference check provide the user with a means of exploring an item's underlying logic from within the program. A commonality check determines single point failures for an item or group of items. Output is in the form of a user-defined matrix or matrices of colored boxes, each box representing an item or set of items from the logic tree. Input is via mouse selection of the matrix boxes, using the mouse buttons to toggle the state of the item. CRANS is written in C-language and requires the MIT X Window System, Version 11 Revision 4 or Revision 5. It requires 78K of RAM for execution and a three button mouse. It has been successfully implemented on Sun4 workstations running SunOS, HP9000 workstations running HP-UX, and DECstations running ULTRIX. No executable is provided on the distribution medium; however

  13. 76 FR 42536 - Real-Time System Management Information Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-19

    ... Federal Highway Administration 23 CFR Part 511 RIN 2125-AF19 Real-Time System Management Information... available and share traffic and travel conditions information via real-time information programs as required... additional comments relating to the costs and benefits of the Real-Time System Management Information...

  14. Real-time support for high performance aircraft operation

    NASA Technical Reports Server (NTRS)

    Vidal, Jacques J.

    1989-01-01

    The feasibility of real-time processing schemes using artificial neural networks (ANNs) is investigated. A rationale for digital neural nets is presented and a general processor architecture for control applications is illustrated. Research results on ANN structures for real-time applications are given. Research results on ANN algorithms for real-time control are also shown.

  15. 17 CFR 38.157 - Real-time market monitoring.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 17 Commodity and Securities Exchanges 1 2014-04-01 2014-04-01 false Real-time market monitoring... DESIGNATED CONTRACT MARKETS Compliance With Rules § 38.157 Real-time market monitoring. A designated contract market must conduct real-time market monitoring of all trading activity on its electronic...

  16. 17 CFR 38.157 - Real-time market monitoring.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 17 Commodity and Securities Exchanges 1 2013-04-01 2013-04-01 false Real-time market monitoring... DESIGNATED CONTRACT MARKETS Compliance With Rules § 38.157 Real-time market monitoring. A designated contract market must conduct real-time market monitoring of all trading activity on its electronic...

  17. Real-time adaptive radiometric compensation.

    PubMed

    Grundhöfer, Anselm; Bimber, Oliver

    2008-01-01

    Recent radiometric compensation techniques make it possible to project images onto colored and textured surfaces. This is realized with projector-camera systems by scanning the projection surface on a per-pixel basis. Using the captured information, a compensation image is calculated that neutralizes geometric distortions and color blending caused by the underlying surface. As a result, the brightness and the contrast of the input image is reduced compared to a conventional projection onto a white canvas. If the input image is not manipulated in its intensities, the compensation image can contain values that are outside the dynamic range of the projector. These will lead to clipping errors and to visible artifacts on the surface. In this article, we present an innovative algorithm that dynamically adjusts the content of the input images before radiometric compensation is carried out. This reduces the perceived visual artifacts while simultaneously preserving a maximum of luminance and contrast. The algorithm is implemented entirely on the GPU and is the first of its kind to run in real-time.

  18. Real-Time Accumulative Computation Motion Detectors

    PubMed Central

    Fernández-Caballero, Antonio; López, María Teresa; Castillo, José Carlos; Maldonado-Bascón, Saturnino

    2009-01-01

    The neurally inspired accumulative computation (AC) method and its application to motion detection have been introduced in the past years. This paper revisits the fact that many researchers have explored the relationship between neural networks and finite state machines. Indeed, finite state machines constitute the best characterized computational model, whereas artificial neural networks have become a very successful tool for modeling and problem solving. The article shows how to reach real-time performance after using a model described as a finite state machine. This paper introduces two steps towards that direction: (a) A simplification of the general AC method is performed by formally transforming it into a finite state machine. (b) A hardware implementation in FPGA of such a designed AC module, as well as an 8-AC motion detector, providing promising performance results. We also offer two case studies of the use of AC motion detectors in surveillance applications, namely infrared-based people segmentation and color-based people tracking, respectively. PMID:22303161

  19. Real-time virtual Doppler ultrasound

    NASA Astrophysics Data System (ADS)

    Khoshniat, Mahdieh; Thorne, Meghan L.; Poepping, Tamie L.; Holdsworth, David W.; Steinman, David A.

    2004-04-01

    Doppler ultrasound (DUS) is widely used to diagnose and plan treatments for vascular diseases, but the relationship between complex blood flow dynamics and the observed DUS signal is not completely understood. In this paper, we demonstrate that Doppler ultrasound can be realistically simulated in a real-time manner via the coupling of a known, previously computed velocity field with a simple model of the ultrasound physics. In the present case a 3D computational fluid dynamics (CFD) model of physiologically pulsatile flow a stenosed carotid bifurcation was interrogated using a sample volume of known geometry and power distribution. Velocity vectors at points within the sample volume were interpolated using a fast geometric search algorithm and, using the specified US probe characteristics and orientation, converted into Doppler shifts for subsequent display as a Doppler spectrogram or color DUS image. The important effect of the intrinsic spectral broadening was simulated by convolving the velocity at each point within the sample volume by a triangle function whose width was proportional to velocity. A spherical sample volume with a Gaussian power distribution was found to be adequate for producing realistic Doppler spectrogram in regions of uniform, jet, and recirculation flow. Fewer than 1000 points seeded uniformly within a radius comprising more than 99% of the total power were required, allowing spectra to be generated from high resolution CFD data at 100Hz frame rates on an inexpensive desktop workstation.

  20. High sensitivity real-time NVR monitor

    NASA Technical Reports Server (NTRS)

    Bowers, William D. (Inventor); Chuan, Raymond L. (Inventor)

    1997-01-01

    A real time non-volatile residue (NVR) monitor, which utilizes surface acoustic wave (SAW) resonators to detect molecular contamination in a given environment. The SAW resonators operate at a resonant frequency of approximately 200 MHz-2,000 MHz which enables the NVR monitor to detect molecular contamination on the order of 10.sup.-11 g-cm.sup.-2 to 10.sup.-13 g-cm.sup.2. The NVR monitor utilizes active temperature control of (SAW) resonators to achieve a stable resonant frequency. The temperature control system of the NVR monitor is able to directly heat and cool the SAW resonators utilizing a thermoelectric element to maintain the resonators at a present temperature independent of the environmental conditions. In order to enable the direct heating and cooling of the SAW resonators, the SAW resonators are operatively mounted to a heat sink. In one embodiment, the heat sink is located in between the SAW resonators and an electronic circuit board which contains at least a portion of the SAW control electronics. The electrical leads of the SAW resonators are connected through the heat sink to the circuit board via an electronic path which prevents inaccurate frequency measurement.

  1. Recommendations for Real-Time Speech MRI

    PubMed Central

    Lingala, Sajan Goud; Sutton, Brad P.; Miquel, Marc E.; Nayak, Krishna S.

    2016-01-01

    Real-time magnetic resonance imaging (RT-MRI) is being increasingly used for speech and vocal production research studies. Several imaging protocols have emerged based on advances in RT-MRI acquisition, reconstruction, and audio-processing methods. This review summarizes the state-of-the-art, discusses technical considerations, and provides specific guidance for new groups entering this field. We provide recommendations for performing RT-MRI of the upper airway. This is a consensus statement stemming from the ISMRM-endorsed Speech MRI summit held in Los Angeles, February 2014. A major unmet need identified at the summit was the need for consensus on protocols that can be easily adapted by researchers equipped with conventional MRI systems. To this end, we provide a discussion of tradeoffs in RT-MRI in terms of acquisition requirements, a priori assumptions, artifacts, computational load, and performance for different speech tasks. We provide four recommended protocols and identify appropriate acquisition and reconstruction tools. We list pointers to open-source software that facilitate implementation. We conclude by discussing current open challenges in the methodological aspects of RT-MRI of speech. PMID:26174802

  2. Real-time DIRCM system modeling

    NASA Astrophysics Data System (ADS)

    Petersson, Mikael

    2004-12-01

    Directed infrared countermeasures (DIRCM) play an increasingly important role in electronic warfare to counteract threats posed by infrared seekers. The usefulness and performance of such countermeasures depend, for example, on atmospheric conditions (attenuation and turbulence) and platform vibrations, causing pointing and tracking errors for the laser beam and reducing the power transferred to the seeker aperture. These problems make it interesting to simulate the performance of a DIRCM system in order to understand how easy or difficult it is to counteract an approaching threat and evaluate limiting factors in various situations. This paper describes a DIRCM model that has been developed, including atmospheric effects such as attenuation and turbulence as well as closed loop tracking algorithms, where the retro reflex of the laser is used for the pointing control of the beam. The DIRCM model is part of a large simulation framework (EWSim), which also incorporates several descriptions of different seekers (e.g. reticle, rosette, centroid, nutating cross) and models of robot dynamics. Effects of a jamming laser on a specific threat can be readily verified by simulations within this framework. The duel between missile and countermeasure is simulated in near real-time and visualized graphically in 3D. A typical simulation with a reticle seeker jammed by a modulated laser is included in the paper.

  3. Near Real Time Ship Detection Experiments

    NASA Astrophysics Data System (ADS)

    Brusch, S.; Lehner, S.; Schwarz, E.; Fritz, T.

    2010-04-01

    A new Near Real Time (NRT) ship detection processor SAINT (SAR AIS Integrated Toolbox) was developed in the framework of the ESA project MARISS. Data are received at DLRs ground segment DLR-BN (Neustrelitz, Germany). Results of the ship detection are available on ftp server within 30 min after the acquisition started. The detectability of ships on Synthetic Aperture Radar (SAR) ERS-2, ENVISAT ASAR and TerraSAR-X (TS-X) images is validated by coastal (live) AIS and space AIS. The monitoring areas chosen for surveillance are the North-, Baltic Sea, and Cape Town. The detectability in respect to environmental parameters like wind field, sea state, currents and changing coastlines due to tidal effects is investigated. In the South Atlantic a tracking experiment of the German research vessel Polarstern has been performed. Issues of piracy in particular in respect to ships hijacked at the Somali coast are discussed. Some examples using high resolution images from TerraSAR-X are given.

  4. Real-Time 3D Visualization

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Butler Hine, former director of the Intelligent Mechanism Group (IMG) at Ames Research Center, and five others partnered to start Fourth Planet, Inc., a visualization company that specializes in the intuitive visual representation of dynamic, real-time data over the Internet and Intranet. Over a five-year period, the then NASA researchers performed ten robotic field missions in harsh climes to mimic the end- to-end operations of automated vehicles trekking across another world under control from Earth. The core software technology for these missions was the Virtual Environment Vehicle Interface (VEVI). Fourth Planet has released VEVI4, the fourth generation of the VEVI software, and NetVision. VEVI4 is a cutting-edge computer graphics simulation and remote control applications tool. The NetVision package allows large companies to view and analyze in virtual 3D space such things as the health or performance of their computer network or locate a trouble spot on an electric power grid. Other products are forthcoming. Fourth Planet is currently part of the NASA/Ames Technology Commercialization Center, a business incubator for start-up companies.

  5. Real-time {sup 90}Sr Counter

    SciTech Connect

    Kaneko, Naomi; Kawai, Hideyuki; Kodama, Satoshi; Kobayashi, Atsushi; Tabata, Makoto; Ito, Hiroshi; Han, Soorim

    2015-07-01

    Radioisotopes have been emitted around Japan due to a nuclear accident at the Fukushima Daiichi nuclear power station in March 2011. A problem is the contaminated water including the atomic nucleus which relatively has a long half- life time and soluble such as {sup 90}Sr, {sup 137}Cs. Internal exposures by {sup 90}Sr are more dangerous than {sup 137}Cs's because Sr has effective half-life time of 18 years and property of accumulation in a born. We have developed real-time {sup 90}Sr counter which is sensitive beta-ray of maximum kinematic energy of 2.28 MeV from {sup 90}Sr and insensitive of beta-ray of maximum kinematic energy of 1.17 MeV and gamma-ray from {sup 90}Sr by Cherenkov detection. This counter composes of Cerenkov counter, trigger scintillation counter and veto counter. Silica aerogel for Cherenkov counter can obtain refractive index between 1.017 and 1.049 easily. And wavelength shifting fiber (WLSF) is used as a light guide for extending effective area and producing lower cost. A mechanism of the identification of {sup 90}Sr is explained in following. In case of {sup 90}Sr, when the trigger counter reacts on the beta-ray from {sup 90}Sr, aerogel emits the Cherenkov light and WLSF reacts and read the Cherenkov light. On the other hand, in case of {sup 137}Cs, the trigger counter reacts on the beta-ray, aerogel stops the beta- ray and Cherenkov light is not emitted. Therefore, aerogel has a function as a radiator and shielding material. the gamma-ray is not reacted on the lower density detector. Cosmic rays would be also reacted by the veto counter. A prototype counter whose the effective area is 30 cm x 10 cm was obtained (2.0±1.2){sup 3} of mis-identification as {sup 137}Cs/{sup 90}Sr. Detection limit in the surface contamination inspection depends on measurement time and effective area mainly. The sensitivity of wide range, 10{sup -2} - 10{sup 4} Bq/cm{sup 2}, is obtained by adjustment of detection level in circuit of this counter. A lower

  6. Apparatus Characterizes Transient Voltages in Real Time

    NASA Technical Reports Server (NTRS)

    Medelius, Pedro

    2005-01-01

    is received, a volatile memory is filled with data for a total time of 200 ms. After the data are transferred to nonvolatile memory, the recorder rearms itself within 400 ms to enable recording of subsequent transients. Unfortunately, the recorded data must be retrieved through a serial communication link. Depending on the amount of data recorded, the memory can be filled before retrieval is completed. Although large amounts of data are recorded and retrieved, only a small part of the information (the selected parameters) is usually required. The present transient-voltage recorder provides the required information, without incurring the overhead associated with the recording, storage, and retrieval of complete transient-waveform data. In operation, this apparatus processes transient voltage waveforms in real time to extract and record the selected parameters. An analog-to-digital converter that operates at a speed of as much as 100 mega-samples per second is used to sample a transient waveform. A real-time comparator and peak detector are implemented by use of fast field-programmable gate arrays.

  7. Benchmarking real-time HEVC streaming

    NASA Astrophysics Data System (ADS)

    Nightingale, James; Wang, Qi; Grecos, Christos

    2012-06-01

    Work towards the standardisation of High Efficiency Video Coding (HEVC), the next generation video coding scheme, is currently gaining pace. HEVC offers the prospect of a 50% improvement in compression over the current H.264 Advanced Video Coding standard (H.264/AVC). Thus far, work on HEVC has concentrated on improvements to the coding efficiency and has not yet addressed transmission in networks other than to mandate byte stream compliance with Annex B of H.264/AVC. For practical networked HEVC applications a number of essential building blocks have yet to be defined. In this work, we design and prototype a real-time HEVC streaming system and empirically evaluate its performance, in particular we consider the robustness of the current Test Model under Consideration (TMuC HM4.0) for HEVC to packet loss caused by a reduction in available bandwidth both in terms of decoder resilience and degradation in perceptual video quality. A NAL unit packetisation and streaming framework for HEVC encoded video streams is designed, implemented and empirically tested in a number of streaming environments including wired, wireless, single path and multiple path network scenarios. As a first step the HEVC decoder's error resilience is tested under a comprehensive set of packet loss conditions and a simple error concealment method for HEVC is implemented. Similarly to H.264 encoded streams, the size and distribution of NAL units within an HEVC stream and the nature of the NAL unit dependencies influences the packetisation and streaming strategies which may be employed for such streams. The relationships between HEVC encoding mode and the quality of the received video are shown under a wide range of bandwidth constraints. HEVC streaming is evaluated in both single and multipath network configuration scenarios. Through the use of extensive experimentation, we establish a comprehensive set of benchmarks for HEVC streaming in loss prone network environments. We show the visual quality

  8. Near real time detection of hazardous airborne substances.

    PubMed

    Leppert, J; Horner, G; Rietz, F; Ringer, J; Schulze Lammers, P; Boeker, P

    2012-11-15

    A fast near real-time monitoring system for hazardous airborne substances, such as chemical warfare agents (CWA) is presented and limits of detection (LOD) for five CW simulants are determined. A tandem thermal desorber (TTD) continuously collects and pre-concentrates air. The pre-concentrated samples are then separated in a fast gas chromatographic (GC) run of 6.9min. and detected by a time-of-flight mass spectrometer (TOFMS). The GC-TOFMS signals are evaluated using chemometric methods for deconvolution and target identification. The high toxicity of nerve agents requires extremely low detection limits; for some as low as 100 ng/m(3) (10 ppt). The combination of TTD, TOFMS and chemometric data evaluation methods enables the system to fulfill this requirement. Calibration measurements for five different CWA simulants show lower limits of detection in the range of 10 ng/m(3)-60 ng/m(3) (1-11 ppt). In addition, the ability to detect trace concentrations of real CWA is demonstrated with a measurement of 30 pg Sarin on column. Several other real CWA measurements are shown, like sulfur mustard in diesel, lewisite under humid conditions and VX. As part of this work the influence of stationary film thickness on peak tailing of organophosphates is investigated for peak shape optimization.

  9. Real-time volumetric scintillation dosimetry

    NASA Astrophysics Data System (ADS)

    Beddar, S.

    2015-01-01

    The goal of this brief review is to review the current status of real-time 3D scintillation dosimetry and what has been done so far in this area. The basic concept is to use a large volume of a scintillator material (liquid or solid) to measure or image the dose distributions from external radiation therapy (RT) beams in three dimensions. In this configuration, the scintillator material fulfills the dual role of being the detector and the phantom material in which the measurements are being performed. In this case, dose perturbations caused by the introduction of a detector within a phantom will not be at issue. All the detector configurations that have been conceived to date used a Charge-Coupled Device (CCD) camera to measure the light produced within the scintillator. In order to accurately measure the scintillation light, one must correct for various optical artefacts that arise as the light propagates from the scintillating centers through the optical chain to the CCD chip. Quenching, defined in its simplest form as a nonlinear response to high-linear energy transfer (LET) charged particles, is one of the disadvantages when such systems are used to measure the absorbed dose from high-LET particles such protons. However, correction methods that restore the linear dose response through the whole proton range have been proven to be effective for both liquid and plastic scintillators. Volumetric scintillation dosimetry has the potential to provide fast, high-resolution and accurate 3D imaging of RT dose distributions. Further research is warranted to optimize the necessary image reconstruction methods and optical corrections needed to achieve its full potential.

  10. Real-Time Feature Tracking Using Homography

    NASA Technical Reports Server (NTRS)

    Clouse, Daniel S.; Cheng, Yang; Ansar, Adnan I.; Trotz, David C.; Padgett, Curtis W.

    2010-01-01

    This software finds feature point correspondences in sequences of images. It is designed for feature matching in aerial imagery. Feature matching is a fundamental step in a number of important image processing operations: calibrating the cameras in a camera array, stabilizing images in aerial movies, geo-registration of images, and generating high-fidelity surface maps from aerial movies. The method uses a Shi-Tomasi corner detector and normalized cross-correlation. This process is likely to result in the production of some mismatches. The feature set is cleaned up using the assumption that there is a large planar patch visible in both images. At high altitude, this assumption is often reasonable. A mathematical transformation, called an homography, is developed that allows us to predict the position in image 2 of any point on the plane in image 1. Any feature pair that is inconsistent with the homography is thrown out. The output of the process is a set of feature pairs, and the homography. The algorithms in this innovation are well known, but the new implementation improves the process in several ways. It runs in real-time at 2 Hz on 64-megapixel imagery. The new Shi-Tomasi corner detector tries to produce the requested number of features by automatically adjusting the minimum distance between found features. The homography-finding code now uses an implementation of the RANSAC algorithm that adjusts the number of iterations automatically to achieve a pre-set probability of missing a set of inliers. The new interface allows the caller to pass in a set of predetermined points in one of the images. This allows the ability to track the same set of points through multiple frames.

  11. Variational optical flow computation in real time.

    PubMed

    Bruhn, Andrés; Weickert, Joachim; Feddern, Christian; Kohlberger, Timo; Schnörr, Christoph

    2005-05-01

    This paper investigates the usefulness of bidirectional multigrid methods for variational optical flow computations. Although these numerical schemes are among the fastest methods for solving equation systems, they are rarely applied in the field of computer vision. We demonstrate how to employ those numerical methods for the treatment of variational optical flow formulations and show that the efficiency of this approach even allows for real-time performance on standard PCs. As a representative for variational optic flow methods, we consider the recently introduced combined local-global method. It can be considered as a noise-robust generalization of the Horn and Schunck technique. We present a decoupled, as well as a coupled, version of the classical Gauss-Seidel solver, and we develop several multgrid implementations based on a discretization coarse grid approximation. In contrast, with standard bidirectional multigrid algorithms, we take advantage of intergrid transfer operators that allow for nondyadic grid hierarchies. As a consequence, no restrictions concerning the image size or the number of traversed levels have to be imposed. In the experimental section, we juxtapose the developed multigrid schemes and demonstrate their superior performance when compared to unidirectional multgrid methods and nonhierachical solvers. For the well-known 316 x 252 Yosemite sequence, we succeeded in computing the complete set of dense flow fields in three quarters of a second on a 3.06-GHz Pentium4 PC. This corresponds to a frame rate of 18 flow fields per second which outperforms the widely-used Gauss-Seidel method by almost three orders of magnitude.

  12. Real time PV manufacturing diagnostic system

    SciTech Connect

    Kochergin, Vladimir; Crawford, Michael A.

    2015-09-01

    The main obstacle Photovoltaic (PV) industry is facing at present is the higher cost of PV energy compared to that of fossil energy. While solar cell efficiencies continue to make incremental gains these improvements are so far insufficient to drive PV costs down to match that of fossil energy. Improved in-line diagnostics however, has the potential to significantly increase the productivity and reduce cost by improving the yield of the process. On this Phase I/Phase II SBIR project MicroXact developed and demonstrated at CIGS pilot manufacturing line a high-throughput in-line PV manufacturing diagnostic system, which was verified to provide fast and accurate data on the spatial uniformity of thickness, an composition of the thin films comprising the solar cell as the solar cell is processed reel-to-reel. In Phase II project MicroXact developed a stand-alone system prototype and demonstrated the following technical characteristics: 1) ability of real time defect/composition inconsistency detection over 60cm wide web at web speeds up to 3m/minute; 2) Better than 1mm spatial resolution on 60cm wide web; 3) an average better than 20nm spectral resolution resulting in more than sufficient sensitivity to composition imperfections (copper-rich and copper-poor regions were detected). The system was verified to be high vacuum compatible. Phase II results completely validated both technical and economic feasibility of the proposed concept. MicroXact’s solution is an enabling technique for in-line PV manufacturing diagnostics to increase the productivity of PV manufacturing lines and reduce the cost of solar energy, thus reducing the US dependency on foreign oil while simultaneously reducing emission of greenhouse gasses.

  13. Real-Time Wireless Data Acquisition System

    NASA Technical Reports Server (NTRS)

    Valencia, Emilio J.; Perotti, Jose; Lucena, Angel; Mata, Carlos

    2007-01-01

    Current and future aerospace requirements demand the creation of a new breed of sensing devices, with emphasis on reduced weight, power consumption, and physical size. This new generation of sensors must possess a high degree of intelligence to provide critical data efficiently and in real-time. Intelligence will include self-calibration, self-health assessment, and pre-processing of raw data at the sensor level. Most of these features are already incorporated in the Wireless Sensors Network (SensorNet(TradeMark)), developed by the Instrumentation Group at Kennedy Space Center (KSC). A system based on the SensorNet(TradeMark) architecture consists of data collection point(s) called Central Stations (CS) and intelligent sensors called Remote Stations (RS) where one or more CSs can be accommodated depending on the specific application. The CS's major function is to establish communications with the Remote Stations and to poll each RS for data and health information. The CS also collects, stores and distributes these data to the appropriate systems requiring the information. The system has the ability to perform point-to-point, multi-point and relay mode communications with an autonomous self-diagnosis of each communications link. Upon detection of a communication failure, the system automatically reconfigures to establish new communication paths. These communication paths are automatically and autonomously selected as the best paths by the system based on the existing operating environment. The data acquisition system currently under development at KSC consists of the SensorNet(TradeMark) wireless sensors as the remote stations and the central station called the Radio Frequency Health Node (RFHN). The RFF1N is the central station which remotely communicates with the SensorNet(TradeMark) sensors to control them and to receive data. The system's salient feature is the ability to provide deterministic sensor data with accurate time stamps for both time critical and non-time

  14. Real time tests for long lead-time forecasting of the magnetic field vectors within CMEs

    NASA Astrophysics Data System (ADS)

    Savani, Neel; Vourlidas, Angelos; Pulkkinen, Antti; Wold, Alexandra M.

    2016-07-01

    The direction of magnetic vectors within coronal mass ejections, CMEs, has significant importance for forecasting terrestrial behavior. We have developed a technique to estimate the time-varying magnetic field at Earth for periods within CMEs (Savani et al 2015, 2016). This technique reduces the complex dynamics in order to create a reliable prediction methodology to operate everyday under robust conditions. In this presentation, we focus on the results and skill scores of the forecasting technique calculated from 40 historical CME events from the pre-STEREO mission. Since these results provided substantial improvements in the long lead-time Kp index forecasts, we have now begun testing under real-time conditions. We will also show the preliminary results of our methodology under these real-time conditions within the CCMC hosted at NASA Goddard Space Flight Center.

  15. Instrumentation development for real time brainwave monitoring.

    SciTech Connect

    Anderson, Lawrence Frederick; Clough, Benjamin W.

    2005-12-01

    The human brain functions through a chemically-induced biological process which operates in a manner similar to electrical systems. The signal resulting from this biochemical process can actually be monitored and read using tools and having patterns similar to those found in electrical and electronics engineering. The primary signature of this electrical activity is the ''brain wave'', which looks remarkably similar to the output of many electrical systems. Likewise, the device currently used in medical arenas to read brain electrical activity is the electroencephalogram (EEG) which is synonymous with a multi-channel oscilloscope reading. Brain wave readings and recordings for medical purposes are traditionally taken in clinical settings such as hospitals, laboratories or diagnostic clinics. The signal is captured via externally applied scalp electrodes using semi-viscous gel to reduce impedance. The signal will be in the 10 to 100 microvolt range. In other instances, where surgeons are attempting to isolate particular types of minute brain signals, the electrodes may actually be temporarily implanted in the brain during a preliminary procedure. The current configurations of equipment required for EEGs involve large recording instruments, many electrodes, wires, and large amounts of hard disk space devoted to storing large files of brain wave data which are then eventually analyzed for patterns of concern. Advances in sensors, signal processing, data storage and microelectronics over the last decade would seem to have paved the way for the realization of devices capable of ''real time'' external monitoring, and possible assessment, of brain activity. A myriad of applications for such a capability are likewise presenting themselves, including the ability to assess brain functioning, level of functioning and malfunctioning. Our plan is to develop the sensors, signal processing, and portable instrumentation package which could capture, analyze, and communicate

  16. Real-time Forensic Disaster Analysis

    NASA Astrophysics Data System (ADS)

    Wenzel, F.; Daniell, J.; Khazai, B.; Mühr, B.; Kunz-Plapp, T.; Markus, M.; Vervaeck, A.

    2012-04-01

    The Center for Disaster Management and Risk Reduction Technology (CEDIM, www.cedim.de) - an interdisciplinary research center founded by the German Research Centre for Geoscience (GFZ) and Karlsruhe Institute of Technology (KIT) - has embarked on a new style of disaster research known as Forensic Disaster Analysis. The notion has been coined by the Integrated Research on Disaster Risk initiative (IRDR, www.irdrinternational.org) launched by ICSU in 2010. It has been defined as an approach to studying natural disasters that aims at uncovering the root causes of disasters through in-depth investigations that go beyond the reconnaissance reports and case studies typically conducted after disasters. In adopting this comprehensive understanding of disasters CEDIM adds a real-time component to the assessment and evaluation process. By comprehensive we mean that most if not all relevant aspects of disasters are considered and jointly analysed. This includes the impact (human, economy, and infrastructure), comparisons with recent historic events, social vulnerability, reconstruction and long-term impacts on livelihood issues. The forensic disaster analysis research mode is thus best characterized as "event-based research" through systematic investigation of critical issues arising after a disaster across various inter-related areas. The forensic approach requires (a) availability of global data bases regarding previous earthquake losses, socio-economic parameters, building stock information, etc.; (b) leveraging platforms such as the EERI clearing house, relief-web, and the many sources of local and international sources where information is organized; and (c) rapid access to critical information (e.g., crowd sourcing techniques) to improve our understanding of the complex dynamics of disasters. The main scientific questions being addressed are: What are critical factors that control loss of life, of infrastructure, and for economy? What are the critical interactions

  17. Real Time Wide Area Radiation Surveillance System

    NASA Astrophysics Data System (ADS)

    Biafore, M.

    2012-04-01

    We present the REWARD project, financed within the FP7 programme, theme SEC-2011.1.5-1 (Development of detection capabilities of difficult to detect radioactive sources and nuclear materials - Capability Project). Within this project, we propose a novel mobile system for real time, wide area radiation surveillance. The system is based on the integration of new miniaturized solid-state radiation sensors: a CdZnTe detector for gamma radiation and a high efficiency neutron detector based on novel silicon technologies. The sensing unit will include a wireless communication interface to send the data remotely to a monitoring base station which also uses a GPS system to calculate the position of the tag. The system will also incorporate middleware and high level software to provide web-service interfaces for the exchange of information, and that will offer top level functionalities as management of users, mobile tags and environment data and alarms, database storage and management and a web-based graphical user interface. Effort will be spent to ensure that the software is modular and re-usable across as many architectural levels as possible. Finally, an expert system will continuously analyze the information from the radiation sensor and correlate it with historical data from the tag location in order to generate an alarm when an abnormal situation is detected. The system will be useful for many different scenarios, including such lost radioactive sources and radioactive contamination. It will be possible to deploy in emergency units and in general in any type of mobile or static equipment. The sensing units will be highly portable thanks to their low size and low energy consumption. The complete system will be scalable in terms of complexity and cost and will offer very high precision on both the measurement and the location of the radiation. The modularity and flexibility of the system will allow for a realistic introduction to the market. Authorities may start with a

  18. PERTS: A Prototyping Environment for Real-Time Systems

    NASA Technical Reports Server (NTRS)

    Liu, Jane W. S.; Lin, Kwei-Jay; Liu, C. L.

    1993-01-01

    PERTS is a prototyping environment for real-time systems. It is being built incrementally and will contain basic building blocks of operating systems for time-critical applications, tools, and performance models for the analysis, evaluation and measurement of real-time systems and a simulation/emulation environment. It is designed to support the use and evaluation of new design approaches, experimentations with alternative system building blocks, and the analysis and performance profiling of prototype real-time systems.

  19. Near Real-Time Processing of Proteomics Data Using Hadoop.

    PubMed

    Hillman, Chris; Ahmad, Yasmeen; Whitehorn, Mark; Cobley, Andy

    2014-03-01

    This article presents a near real-time processing solution using MapReduce and Hadoop. The solution is aimed at some of the data management and processing challenges facing the life sciences community. Research into genes and their product proteins generates huge volumes of data that must be extensively preprocessed before any biological insight can be gained. In order to carry out this processing in a timely manner, we have investigated the use of techniques from the big data field. These are applied specifically to process data resulting from mass spectrometers in the course of proteomic experiments. Here we present methods of handling the raw data in Hadoop, and then we investigate a process for preprocessing the data using Java code and the MapReduce framework to identify 2D and 3D peaks.

  20. Fast Simulation of Tsunamis in Real Time

    NASA Astrophysics Data System (ADS)

    Fryer, G. J.; Wang, D.; Becker, N. C.; Weinstein, S. A.; Walsh, D.

    2011-12-01

    The U.S. Tsunami Warning Centers primarily base their wave height forecasts on precomputed tsunami scenarios, such as the SIFT model (Standby Inundation Forecasting of Tsunamis) developed by NOAA's Center for Tsunami Research. In SIFT, tsunami simulations for about 1600 individual earthquake sources, each 100x50 km, define shallow subduction worldwide. These simulations are stored in a database and combined linearly to make up the tsunami from any great earthquake. Precomputation is necessary because the nonlinear shallow-water wave equations are too time consuming to compute during an event. While such scenario-based models are valuable, they tacitly assume all energy in a tsunami comes from thrust at the décollement. The thrust assumption is often violated (e.g., 1933 Sanriku, 2007 Kurils, 2009 Samoa), while a significant number of tsunamigenic earthquakes are completely unrelated to subduction (e.g., 1812 Santa Barbara, 1939 Accra, 1975 Kalapana). Finally, parts of some subduction zones are so poorly defined that precomputations may be of little value (e.g., 1762 Arakan, 1755 Lisbon). For all such sources, a fast means of estimating tsunami size is essential. At the Pacific Tsunami Warning Center, we have been using our model RIFT (Real-time Inundation Forecasting of Tsunamis) experimentally for two years. RIFT is fast by design: it solves only the linearized form of the equations. At 4 arc-minutes resolution calculations for the entire Pacific take just a few minutes on an 8-processor Linux box. Part of the rationale for developing RIFT was earthquakes of M 7.8 or smaller, which approach the lower limit of the more complex SIFT's abilities. For such events we currently issue a fixed warning to areas within 1,000 km of the source, which typically means a lot of over-warning. With sources defined by W-phase CMTs, exhaustive comparison with runup data shows that we can reduce the warning area significantly. Even before CMTs are available, we routinely run models

  1. The Near Real Time Information System.

    DTIC Science & Technology

    1988-03-01

    process must strive to attain. These goals are as follows: * deployability, 26 1 11 * reliable and efficient systems, * complementary systems, * near real...is maximized. The optimum design of a system is one that is self-deployable or one that is wholly contained in a self-deployable system. Reliable and... reliable and dependable in any environment, on any terrain. Any supporting component of a system must be easily replaceable and not so critical that

  2. Real Time Flux Control in PM Motors

    SciTech Connect

    Otaduy, P.J.

    2005-09-27

    Significant research at the Oak Ridge National Laboratory (ORNL) Power Electronics and Electric Machinery Research Center (PEEMRC) is being conducted to develop ways to increase (1) torque, (2) speed range, and (3) efficiency of traction electric motors for hybrid electric vehicles (HEV) within existing current and voltage bounds. Current is limited by the inverter semiconductor devices' capability and voltage is limited by the stator wire insulation's ability to withstand the maximum back-electromotive force (emf), which occurs at the upper end of the speed range. One research track has been to explore ways to control the path and magnitude of magnetic flux while the motor is operating. The phrase, real time flux control (RTFC), refers to this mode of operation in which system parameters are changed while the motor is operating to improve its performance and speed range. RTFC has potential to meet an increased torque demand by introducing additional flux through the main air gap from an external source. It can augment the speed range by diverting flux away from the main air gap to reduce back-emf at high speeds. Conventional RTFC technology is known as vector control [1]. Vector control decomposes the stator current into two components; one that produces torque and a second that opposes (weakens) the magnetic field generated by the rotor, thereby requiring more overall stator current and reducing the efficiency. Efficiency can be improved by selecting a RTFC method that reduces the back-emf without increasing the average current. This favors methods that use pulse currents or very low currents to achieve field weakening. Foremost in ORNL's effort to develop flux control is the work of J. S. Hsu. Early research [2,3] introduced direct control of air-gap flux in permanent magnet (PM) machines and demonstrated it with a flux-controlled generator. The configuration eliminates the problem of demagnetization because it diverts all the flux from the magnets instead of

  3. Real-Time Polymerase Chain Reaction Assays for Rickettsial Diseases

    DTIC Science & Technology

    2004-06-01

    agents in the blood stream the diseases are also difficult to diagnose by laboratory methods. For that reason we have developed real - time PCR assays to...detect rickettsial disease agents both at the genus and the species level. Real - time PCR assays were developed to identify: 1) pathogenic Rickettsia...calculate the sensitivity of the assays. These real - time PCR assays were found to be capable of detecting rickettsial disease agents quickly and with great sensitivity and specificity.

  4. Extensions to Real-time Hierarchical Mine Detection Algorithm

    DTIC Science & Technology

    2002-09-01

    Extensions to Real-Time Hierarchical Mine Detection Algorithm System Number: Patron Number: Requester: Notes: DSIS Use only: Deliver to: DK...Recherche et developpement pour Ia defense Canada Extensions to Real-Time Hierarchical Mine Detection Algorithm Final Report Sinh Duong and Mabo R. Ito...EXTENSIONS TO REAL-TIME HIERARCHICAL MINE DETECTION ALGORITHM FINAL REPORT by Smh Duong and Mabo R Ito The Univer~ity of Bntl~h Columbia Vancouver

  5. Real-Time Trajectory Generation for Autonomous Nonlinear Flight Systems

    DTIC Science & Technology

    2006-04-01

    Real-Time Trajectory Generation for Autonomous Nonlinear Flight Systems AF02T002 Phase II Final Report Contract No. FA9550-04-C-0032 Principal...3. REPORT TYPE AND DATES COVERED Final Report for 14 April 2004-14 April 2006 Real-Time Trajectory Generation for Autonomous Nonlinear Flight...A 13. ABSTRACT (Maximum 200 Words) Unmanned aerial vehicle and smart munition systems need robust, real-time path generation and

  6. A Real-Time Linux for Multicore Platforms

    DTIC Science & Technology

    2013-12-20

    B . Chattopadhyay , S. Baruah. A lookup-table driven approach to partitioned scheduling, IEEE Real-Time Technology and Applications Symposium...reviewed journals: Number of Papers published in non peer-reviewed journals: ( b ) Papers published in non-peer-reviewed journals (N/ A for none) James H...Herman, B . Brandenburg, A . Mills, J. Anderson. Soft Real-Time on Multiprocessors:Are Analysis-Based Schedulers ReallyWorth It?, 32nd IEEE Real-Time

  7. Real-Time MENTAT programming language and architecture

    NASA Technical Reports Server (NTRS)

    Grimshaw, Andrew S.; Silberman, Ami; Liu, Jane W. S.

    1989-01-01

    Real-time MENTAT, a programming environment designed to simplify the task of programming real-time applications in distributed and parallel environments, is described. It is based on the same data-driven computation model and object-oriented programming paradigm as MENTAT. It provides an easy-to-use mechanism to exploit parallelism, language constructs for the expression and enforcement of timing constraints, and run-time support for scheduling and exciting real-time programs. The real-time MENTAT programming language is an extended C++. The extensions are added to facilitate automatic detection of data flow and generation of data flow graphs, to express the timing constraints of individual granules of computation, and to provide scheduling directives for the runtime system. A high-level view of the real-time MENTAT system architecture and programming language constructs is provided.

  8. Dynamic multiplanar real time ultrasound guided infraclavicular subclavian vein catheterization.

    PubMed

    Zhong, Xin; Hamill, Mark; Collier, Bryan; Bradburn, Eric; Ferrara, John

    2015-06-01

    Ultrasound guided vascular access has been well-characterized as a safe and effective technique for internal jugular and femoral vein catheterization. However, there is limited experience with the use of ultrasound to access the infraclavicular subclavian vein. Multiple ultrasound techniques do exist to identify the subclavian vein, but real time access is limited by vessel identification in a single planar view. To overcome this limitation, a novel technique of ultrasound guided infraclavicular subclavian vein catheterization using a real time multiplanar approach has been developed. The initial experience with this approach is described. A single surgeon used combined oblique, transverse, and longitudinal views along with Doppler color flow images to both define the infraclavicular anatomy and to obtain subclavian vein access in 42 adult patients (20 M/22 F and 22 L/20 R) with a mean body mass index of 29.2 (range = 18.9-55.4). Chest x-ray was obtained to confirm position and to rule out pneumothorax. Subclavian vein cannulation was achieved in 100 per cent of patients; subsequent catheterization was successful in 92.9 per cent. The number of attempts required for cannulation averaged 1.3 (range = 1-5), and decreased after a five patient learning curve. No patient developed a pneumothorax, hematoma, or cannula malposition. Ultrasound guided multiplanar infraclavicular subclavian vein access appears to be a safe and effective adjunct for central line placement.

  9. Real time simulation using position sensing

    NASA Technical Reports Server (NTRS)

    Studor, George F. (Inventor); Womack, Robert W. (Inventor); Hilferty, Michael F. (Inventor); Isbell, William B. (Inventor); Taylor, Jason A. (Inventor); Bacon, Bruce R. (Inventor)

    2000-01-01

    An interactive exercise system including exercise equipment having a resistance system, a speed sensor, a controller that varies the resistance setting of the exercise equipment, and a playback device for playing pre-recorded video and audio. The controller, operating in conjunction with speed information from the speed sensor and terrain information from media table files, dynamically varies the resistance setting of the exercise equipment in order to simulate varying degrees of difficulty while the playback device concurrently plays back the video and audio to create the simulation that the user is exercising in a natural setting such as a real-world exercise course.

  10. A Circuit Model of Real Time Human Body Hydration.

    PubMed

    Asogwa, Clement Ogugua; Teshome, Assefa K; Collins, Stephen F; Lai, Daniel T H

    2016-06-01

    Changes in human body hydration leading to excess fluid losses or overload affects the body fluid's ability to provide the necessary support for healthy living. We propose a time-dependent circuit model of real-time human body hydration, which models the human body tissue as a signal transmission medium. The circuit model predicts the attenuation of a propagating electrical signal. Hydration rates are modeled by a time constant τ, which characterizes the individual specific metabolic function of the body part measured. We define a surrogate human body anthropometric parameter θ by the muscle-fat ratio and comparing it with the body mass index (BMI), we find theoretically, the rate of hydration varying from 1.73 dB/min, for high θ and low τ to 0.05 dB/min for low θ and high τ. We compare these theoretical values with empirical measurements and show that real-time changes in human body hydration can be observed by measuring signal attenuation. We took empirical measurements using a vector network analyzer and obtained different hydration rates for various BMI, ranging from 0.6 dB/min for 22.7 [Formula: see text] down to 0.04 dB/min for 41.2 [Formula: see text]. We conclude that the galvanic coupling circuit model can predict changes in the volume of the body fluid, which are essential in diagnosing and monitoring treatment of body fluid disorder. Individuals with high BMI would have higher time-dependent biological characteristic, lower metabolic rate, and lower rate of hydration.

  11. Casi real-time surface-laid mine detection system

    NASA Astrophysics Data System (ADS)

    Ivanco, Tyler; Achal, Stephen B.; McFee, John E.; Anger, Clifford D.

    2001-10-01

    A ground vehicle-based, real-time, surface mine detection system, utilizing a Compact Airborne Spectrographic Image (casi), efficient mine detection algorithms, and real-time processing systems, was designed and tested. The combined real-time system was capable of 'learning' the in-situ spectra of various mines, thus providing a spectral library for the detection algorithms. The real-time processing of the casi data involved three steps. The first step was the radiometric correction of the raw data. The second step involved the application of the mine detection algorithms to the corrected data, referencing the spectral library. In the final step, the results of the real-time processes were stored and displayed, usually within a few frame times of the data acquisition. To the authors knowledge, this system represents the first hyperspectral imager to detect mines in real-time. This paper describes the generation of the in-situ mine spectral library, the collection of the scene data, the real-time processing of the scene data and the subsequent display and recording of the detection data. The limitation and expansion capabilities of the real-time system are discussed as well as various techniques that were implemented to achieve the goals. Planned future improvements that have been identified to create a more robust and higher performance, yet simpler processing systems are also discussed.

  12. Real-time fault diagnosis for propulsion systems

    NASA Technical Reports Server (NTRS)

    Merrill, Walter C.; Guo, Ten-Huei; Delaat, John C.; Duyar, Ahmet

    1991-01-01

    Current research toward real time fault diagnosis for propulsion systems at NASA-Lewis is described. The research is being applied to both air breathing and rocket propulsion systems. Topics include fault detection methods including neural networks, system modeling, and real time implementations.

  13. Real-time hyperspectral imaging for food safety applications

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Multispectral imaging systems with selected bands can commonly be used for real-time applications of food processing. Recent research has demonstrated several image processing methods including binning, noise removal filter, and appropriate morphological analysis in real-time mode can remove most fa...

  14. REAL-TIME ENVIRONMENTAL MONITORING: APPLICATIONS FOR HOMELAND SECURITY

    EPA Science Inventory

    Real-time monitoring technology developed as part of the EMPACT program has a variety of potential applications. These tools can measure a variety of potential contaminants in the air, water, in buildings, or in the soil. Real-time monitoring technology allows these detection sys...

  15. "Real-Time" Case Studies in Organizational Communication

    ERIC Educational Resources Information Center

    Long, Shawn D.

    2005-01-01

    This article presents an activity that integrates theory and application by examining the multiple communication events affecting a single organization in "real time" over the course of an academic term. The "real-time" case study (RTCS) avails students of the opportunity to examine organizational communication events as they are occurring in…

  16. Recent achievements in real-time computational seismology in Taiwan

    NASA Astrophysics Data System (ADS)

    Lee, S.; Liang, W.; Huang, B.

    2012-12-01

    Real-time computational seismology is currently possible to be achieved which needs highly connection between seismic database and high performance computing. We have developed a real-time moment tensor monitoring system (RMT) by using continuous BATS records and moment tensor inversion (CMT) technique. The real-time online earthquake simulation service is also ready to open for researchers and public earthquake science education (ROS). Combine RMT with ROS, the earthquake report based on computational seismology can provide within 5 minutes after an earthquake occurred (RMT obtains point source information < 120 sec; ROS completes a 3D simulation < 3 minutes). All of these computational results are posted on the internet in real-time now. For more information, welcome to visit real-time computational seismology earthquake report webpage (RCS).

  17. Hardware for a real-time multiprocessor simulator

    NASA Technical Reports Server (NTRS)

    Blech, R. A.; Arpasi, D. J.

    1984-01-01

    The hardware for a real time multiprocessor simulator (RTMPS) developed at the NASA Lewis Research Center is described. The RTMPS is a multiple microprocessor system used to investigate the application of parallel processing concepts to real time simulation. It is designed to provide flexible data exchange paths between processors by using off the shelf microcomputer boards and minimal customized interfacing. A dedicated operator interface allows easy setup of the simulator and quick interpreting of simulation data. Simulations for the RTMPS are coded in a NASA designed real time multiprocessor language (RTMPL). This language is high level and geared to the multiprocessor environment. A real time multiprocessor operating system (RTMPOS) has also been developed that provides a user friendly operator interface. The RTMPS and supporting software are currently operational and are being evaluated at Lewis. The results of this evaluation will be used to specify the design of an optimized parallel processing system for real time simulation of dynamic systems.

  18. Recent advances to obtain real - Time displacements for engineering applications

    USGS Publications Warehouse

    Celebi, M.

    2005-01-01

    This paper presents recent developments and approaches (using GPS technology and real-time double-integration) to obtain displacements and, in turn, drift ratios, in real-time or near real-time to meet the needs of the engineering and user community in seismic monitoring and assessing the functionality and damage condition of structures. Drift ratios computed in near real-time allow technical assessment of the damage condition of a building. Relevant parameters, such as the type of connections and story structural characteristics (including geometry) are used in computing drifts corresponding to several pre-selected threshold stages of damage. Thus, drift ratios determined from real-time monitoring can be compared to pre-computed threshold drift ratios. The approaches described herein can be used for performance evaluation of structures and can be considered as building health-monitoring applications.

  19. A real-time simulator of a turbofan engine

    NASA Technical Reports Server (NTRS)

    Litt, Jonathan S.; Delaat, John C.; Merrill, Walter C.

    1989-01-01

    A real-time digital simulator of a Pratt and Whitney F100 engine has been developed for real-time code verification and for actuator diagnosis during full-scale engine testing. This self-contained unit can operate in an open-loop stand-alone mode or as part of closed-loop control system. It can also be used for control system design and development. Tests conducted in conjunction with the NASA Advanced Detection, Isolation, and Accommodation program show that the simulator is a valuable tool for real-time code verification and as a real-time actuator simulator for actuator fault diagnosis. Although currently a small perturbation model, advances in microprocessor hardware should allow the simulator to evolve into a real-time, full-envelope, full engine simulation.

  20. Real-Time MEG Source Localization Using Regional Clustering.

    PubMed

    Dinh, Christoph; Strohmeier, Daniel; Luessi, Martin; Güllmar, Daniel; Baumgarten, Daniel; Haueisen, Jens; Hämäläinen, Matti S

    2015-11-01

    With its millisecond temporal resolution, Magnetoencephalography (MEG) is well suited for real-time monitoring of brain activity. Real-time feedback allows the adaption of the experiment to the subject's reaction and increases time efficiency by shortening acquisition and off-line analysis. Two formidable challenges exist in real-time analysis: the low signal-to-noise ratio (SNR) and the limited time available for computations. Since the low SNR reduces the number of distinguishable sources, we propose an approach which downsizes the source space based on a cortical atlas and allows to discern the sources in the presence of noise. Each cortical region is represented by a small set of dipoles, which is obtained by a clustering algorithm. Using this approach, we adapted dynamic statistical parametric mapping for real-time source localization. In terms of point spread and crosstalk between regions the proposed clustering technique performs better than selecting spatially evenly distributed dipoles. We conducted real-time source localization on MEG data from an auditory experiment. The results demonstrate that the proposed real-time method localizes sources reliably in the superior temporal gyrus. We conclude that real-time source estimation based on MEG is a feasible, useful addition to the standard on-line processing methods, and enables feedback based on neural activity during the measurements.

  1. Real-Time MEG Source Localization using Regional Clustering

    PubMed Central

    Dinh, Christoph; Strohmeier, Daniel; Luessi, Martin; Güllmar, Daniel; Baumgarten, Daniel; Haueisen, Jens; Hämäläinen, Matti S.

    2015-01-01

    With its millisecond temporal resolution, Magnetoencephalography (MEG) is well suited for real-time monitoring of brain activity. Real-time feedback allows the adaption of the experiment to the subject’s reaction and increases time efficiency by shortening acquisition and offline analysis. Two formidable challenges exist in real-time analysis: the low signal-to-noise ratio (SNR) and the limited time available for computations. Since the low SNR reduces the number of distinguishable sources, we propose an approach which downsizes the source space based on a cortical atlas and allows to discern the sources in the presence of noise. Each cortical region is represented by a small set of dipoles, which is obtained by a clustering algorithm. Using this approach, we adapted dynamic statistical parametric mapping (dSPM) for real-time source localization. In terms of point spread and crosstalk between regions the proposed clustering technique performs better than selecting spatially evenly distributed dipoles. We conducted real-time source localization on MEG data from an auditory experiment. The results demonstrate that the proposed real-time method localizes sources reliably in the superior temporal gyrus. We conclude that real-time source estimation based on MEG is a feasible, useful addition to the standard on-line processing methods, and enables feedback based on neural activity during the measurements. PMID:25782980

  2. Reviewing real-time performance of nuclear reactor safety systems

    SciTech Connect

    Preckshot, G.G.

    1993-08-01

    The purpose of this paper is to recommend regulatory guidance for reviewers examining real-time performance of computer-based safety systems used in nuclear power plants. Three areas of guidance are covered in this report. The first area covers how to determine if, when, and what prototypes should be required of developers to make a convincing demonstration that specific problems have been solved or that performance goals have been met. The second area has recommendations for timing analyses that will prove that the real-time system will meet its safety-imposed deadlines. The third area has description of means for assessing expected or actual real-time performance before, during, and after development is completed. To ensure that the delivered real-time software product meets performance goals, the paper recommends certain types of code-execution and communications scheduling. Technical background is provided in the appendix on methods of timing analysis, scheduling real-time computations, prototyping, real-time software development approaches, modeling and measurement, and real-time operating systems.

  3. Expert systems for real-time monitoring and fault diagnosis

    NASA Technical Reports Server (NTRS)

    Edwards, S. J.; Caglayan, A. K.

    1989-01-01

    Methods for building real-time onboard expert systems were investigated, and the use of expert systems technology was demonstrated in improving the performance of current real-time onboard monitoring and fault diagnosis applications. The potential applications of the proposed research include an expert system environment allowing the integration of expert systems into conventional time-critical application solutions, a grammar for describing the discrete event behavior of monitoring and fault diagnosis systems, and their applications to new real-time hardware fault diagnosis and monitoring systems for aircraft.

  4. Real-Time Geospatial Data Viewer (RETIGO)

    EPA Science Inventory

    This is a web-based method that allows the users to upload their air monitoring data and explore the data on graphical interface. The method is optimized for mobile monitoring data sets, showing the data on a map, on a time series, and referenced to a hypothesized line and/or poi...

  5. Novor: Real-Time Peptide de Novo Sequencing Software

    NASA Astrophysics Data System (ADS)

    Ma, Bin

    2015-11-01

    De novo sequencing software has been widely used in proteomics to sequence new peptides from tandem mass spectrometry data. This study presents a new software tool, Novor, to greatly improve both the speed and accuracy of today's peptide de novo sequencing analyses. To improve the accuracy, Novor's scoring functions are based on two large decision trees built from a peptide spectral library with more than 300,000 spectra with machine learning. Important knowledge about peptide fragmentation is extracted automatically from the library and incorporated into the scoring functions. The decision tree model also enables efficient score calculation and contributes to the speed improvement. To further improve the speed, a two-stage algorithmic approach, namely dynamic programming and refinement, is used. The software program was also carefully optimized. On the testing datasets, Novor sequenced 7%-37% more correct residues than the state-of-the-art de novo sequencing tool, PEAKS, while being an order of magnitude faster. Novor can de novo sequence more than 300 MS/MS spectra per second on a laptop computer. The speed surpasses the acquisition speed of today's mass spectrometer and, therefore, opens a new possibility to de novo sequence in real time while the spectrometer is acquiring the spectral data.

  6. An Automated Real Time Capillary Viscometer

    DTIC Science & Technology

    2007-11-02

    blood viscosity accounts for clinical manifestations of Polycythemia that have been observed in approximately 50 % of newborn infants with...Merlob and S.H. Reisner, "Neonatal Polycythemia . I. Early diagnosis and incidence relating to time of sampling," Pediatrics, vol. 73, pp. 7-10, 1984...10] T.E. Wiswell, J.D. Cornish and R.S. Northam, "Neonatal Polycythemia : frequency of clinical manifestations and other associated findings

  7. Real-time adaptive aircraft scheduling

    NASA Technical Reports Server (NTRS)

    Kolitz, Stephan E.; Terrab, Mostafa

    1990-01-01

    One of the most important functions of any air traffic management system is the assignment of ground-holding times to flights, i.e., the determination of whether and by how much the take-off of a particular aircraft headed for a congested part of the air traffic control (ATC) system should be postponed in order to reduce the likelihood and extent of airborne delays. An analysis is presented for the fundamental case in which flights from many destinations must be scheduled for arrival at a single congested airport; the formulation is also useful in scheduling the landing of airborne flights within the extended terminal area. A set of approaches is described for addressing a deterministic and a probabilistic version of this problem. For the deterministic case, where airport capacities are known and fixed, several models were developed with associated low-order polynomial-time algorithms. For general delay cost functions, these algorithms find an optimal solution. Under a particular natural assumption regarding the delay cost function, an extremely fast (O(n ln n)) algorithm was developed. For the probabilistic case, using an estimated probability distribution of airport capacities, a model was developed with an associated low-order polynomial-time heuristic algorithm with useful properties.

  8. Toward Real Time Uavs' Image Mosaicking

    NASA Astrophysics Data System (ADS)

    Mehrdad, S.; Satari, M.; Safdary, M.; Moallem, P.

    2016-06-01

    Anyone knows that sudden catastrophes can instantly do great damage. Fast and accurate acquisition of catastrophe information is an essential task for minimize life and property damage. Compared with other ways of catastrophe data acquisition, UAV based platforms can optimize time, cost and accuracy of the data acquisition, as a result UAVs' data has become the first choice in such condition. In this paper, a novel and fast strategy is proposed for registering and mosaicking of UAVs' image data. Firstly, imprecise image positions are used to find adjoining frames. Then matching process is done by a novel matching method. With keeping Sift in mind, this fast matching method is introduced, which uses images exposure time geometry, SIFT point detector and rBRIEF descriptor vector in order to match points efficiency, and by efficiency we mean not only time efficiency but also elimination of mismatch points. This method uses each image sequence imprecise attitude in order to use Epipolar geometry to both restricting search space of matching and eliminating mismatch points. In consideration of reaching to images imprecise attitude and positions we calibrated the UAV's sensors. After matching process, RANSAC is used to eliminate mismatched tie points. In order to obtain final mosaic, image histograms are equalized and a weighted average method is used to image composition in overlapping areas. The total RMSE over all matching points is 1.72 m.

  9. Direct Real-Time Detection of Vapors from Explosive Compounds

    SciTech Connect

    Ewing, Robert G.; Clowers, Brian H.; Atkinson, David A.

    2013-10-03

    The real-time detection of vapors from low volatility explosives including PETN, tetryl, RDX and nitroglycerine along with various compositions containing these substances is demonstrated. This was accomplished with an atmospheric flow tube (AFT) using a non-radioactive ionization source and coupled to a mass spectrometer. Direct vapor detection was demonstrated in less than 5 seconds at ambient temperature without sample pre-concentration. The several seconds of residence time of analytes in the AFT provides a significant opportunity for reactant ions to interact with analyte vapors to achieve ionization. This extended reaction time, combined with the selective ionization using the nitrate reactant ions (NO3- and NO3-•HNO3), enables highly sensitive explosives detection. Observed signals from diluted explosive vapors indicate detection limits below 10 ppqv using selected ion monitoring (SIM) of the explosive-nitrate adduct at m/z 349, 378, 284 and 289 for tetryl, PETN, RDX and NG respectively. Also provided is a demonstration of the vapor detection from 10 different energetic formulations, including double base propellants, plastic explosives and commercial blasting explosives using SIM for the NG, PETN and RDX product ions.

  10. Real-time application of the drag based model

    NASA Astrophysics Data System (ADS)

    Žic, Tomislav; Temmer, Manuela; Vršnak, Bojan

    2016-04-01

    The drag-based model (DBM) is an analytical model which is usually used for calculating kinematics of coronal mass ejections (CMEs) in the interplanetary space, prediction of the CME arrival times and impact speeds at arbitrary targets in the heliosphere. The main assumption of the model is that beyond a distance of about 20 solar radii from the Sun, the drag is dominant in the interplanetary space. The previous version of DBM relied on the rough assumption of averaged, unperturbed and constant environmental conditions as well as constant CME properties throughout the entire interplanetary CME propagation. The continuation of our work consists of enhancing the model into a form which uses a time dependent and perturbed environment without constraints on CME properties and distance forecasting. The extension provides the possibility of application in various scenarios, such as automatic least-square fitting on initial CME kinematic data suitable for a real-time forecasting of CME kinematics, or embedding the DBM into pre-calculated interplanetary ambient conditions provided by advanced numerical simulations (for example, codes of ENLIL, EUHFORIA, etc.). A demonstration of the enhanced DBM is available on the web-site: http://www.geof.unizg.hr/~tzic/dbm.html. We acknowledge the support of European Social Fund under the "PoKRet" project.

  11. Faster and cleaner real-time pure shift NMR experiments.

    PubMed

    Mauhart, Johannes; Glanzer, Simon; Sakhaii, Peyman; Bermel, Wolfgang; Zangger, Klaus

    2015-10-01

    Real-time pure shift experiments provide highly resolved proton NMR spectra which do not require any special processing. Although being more sensitive than their pseudo 2D counterparts, their signal intensities per unit time are still far below regular NMR spectra. In addition, scalar coupling evolution during the individual data chunks produces decoupling sidebands. Here we show that faster and cleaner real-time pure shift spectra can be obtained through the implementation of two parameter alterations. Variation of the FID chunk lengths between individual transients significantly suppresses decoupling sidebands for any kind of real-time pure shift spectra and thus allows for example the analysis of minor components in compound mixtures. Shifting the excitation frequency between individual scans of real-time slice-selective pure shift spectra increases their sensitivity obtainable in unit time by allowing faster repetitions of acquisitions.

  12. Space Shuttle Main Engine real time stability analysis

    NASA Technical Reports Server (NTRS)

    Kuo, F. Y.

    1993-01-01

    The Space Shuttle Main Engine (SSME) is a reusable, high performance, liquid rocket engine with variable thrust. The engine control system continuously monitors the engine parameters and issues propellant valve control signals in accordance with the thrust and mixture ratio commands. A real time engine simulation lab was installed at MSFC to verify flight software and to perform engine dynamic analysis. A real time engine model was developed on the AD100 computer system. This model provides sufficient fidelity on the dynamics of major engine components and yet simplified enough to be executed in real time. The hardware-in-the-loop type simulation and analysis becomes necessary as NASA is continuously improving the SSME technology, some with significant changes in the dynamics of the engine. The many issues of interfaces between new components and the engine can be better understood and be resolved prior to the firing of the engine. In this paper, the SSME real time simulation Lab at the MSFC, the SSME real time model, SSME engine and control system stability analysis, both in real time and non-real time is presented.

  13. Vector processing enhancements for real-time image analysis.

    SciTech Connect

    Shoaf, S.; APS Engineering Support Division

    2008-01-01

    A real-time image analysis system was developed for beam imaging diagnostics. An Apple Power Mac G5 with an Active Silicon LFG frame grabber was used to capture video images that were processed and analyzed. Software routines were created to utilize vector-processing hardware to reduce the time to process images as compared to conventional methods. These improvements allow for more advanced image processing diagnostics to be performed in real time.

  14. Black Hole Formation in Real Time

    NASA Astrophysics Data System (ADS)

    Nissanke, Samaya

    2015-08-01

    Gravity plays a fundamental role in the formation, evolution and fate of stars. However, it remains unclear how massive stars, almost always in pairs, end their lives as extreme gravity objects (neutron stars and black holes) and what their eventual fate is. The physics driving these events in strong-field gravity are complex, rich but still remain elusive. Theoretical work in general relativity has long predicted that the formation of black holes through neutron star mergers emit vast amounts of gravitational radiation, through gravitational waves (GWs), and conventional electromagnetic (EM) radiation. Observing GWs and EM radiation from these elusive short-lived mergers remains one of the holy grails of modern astronomy and is only now possible with a suite of new time-domain telescopes and experiments. I will first review the most recent advances in this blossoming field of EM+GW astronomy, which combines three active disciplines: time-domain astronomy, computational astrophysics and general relativity. I will discuss the promises of this new convergence by illustrating the wealth of astrophysical information that a combined EM+GW measurement would immediately bring. I will then outline the main challenges that lie ahead for this new field in pinpointing the sky location of neutron star mergers using GW detectors and optical and radio wide-field synoptic surveys.

  15. PERFORMANCE RESULTS OF JET-REMPI AS A REAL-TIME PCDD/F EMISSION MONITOR

    EPA Science Inventory

    The Jet REMPI monitor was recently tested on a hazardous-waste firing boiler for its ability to determine real time concentrations of polychlorinated dibenzodioxins and dibenzofurans (PCDDs/Fs). Jet REMPI consists of a laser system coupled with a time of flight mass spectrometer ...

  16. Real-time cosmography with redshift derivatives

    NASA Astrophysics Data System (ADS)

    Martins, C. J. A. P.; Martinelli, M.; Calabrese, E.; Ramos, M. P. L. P.

    2016-08-01

    The drift in the redshift of objects passively following the cosmological expansion has long been recognized as a key model-independent probe of cosmology. Here, we study the cosmological relevance of measurements of time or redshift derivatives of this drift, arguing that the combination of first and second redshift derivatives is a powerful test of the Λ CDM cosmological model. In particular, the latter can be obtained numerically from a set of measurements of the drift at different redshifts. We show that, in the low-redshift limit, a measurement of the derivative of the drift can provide a constraint on the jerk parameter, which is j =1 for flat Λ CDM , while generically j ≠1 for other models. We emphasize that such a measurement is well within the reach of the ELT-HIRES and SKA Phase 2 array surveys.

  17. Real time control for NASA robotic gripper

    NASA Technical Reports Server (NTRS)

    Salter, Carole A.; Baras, John S.

    1989-01-01

    Control laws (in some optimal sense) are being developed for the gripper/nut runner end effector. Control laws for the gripper and nut runner portions of the end effector may be developed independently since these two systems are decoupled. A hybrid force/position controller will be used for both the gripper and nut runner. The development of the gripper controller is explained. Sensory data available to the controller is obtained from an array of strain gages as well as a linear potentiometer. Applying well known optimal control theoretic principles, the control which minimizes the transition time between positions is obtained. In addition, a robust force control scheme is developed to contend with the strain gage drift caused by extreme temperature variations encountered in space.

  18. Real time control for NASA robotic gripper

    NASA Technical Reports Server (NTRS)

    Salter, Carole A.; Baras, John S.

    1990-01-01

    The ability to easily manipulate objects in a zero gravity environment will pay a key role in future space activities. Emphasis will be placed on robotic manipulation. This will serve to increase astronaut safety and utility in addition to several other benefits. The aim is to develop control laws for the zero gravity robotic end effectors. A hybrid force/position controller will be used. Sensory data available to the controller are obtained from an array of strain gauges and a linear potentiometer. Applying well known optimal control theoretical principles, the control which minimizes the transition time between positions is obtained. A robust force control scheme is developed which allows the desired holding force to be achieved smoothly without oscillation. In addition, an algorithm is found to determine contact force and contact location.

  19. Real-time dynamic simulation of the Cassini spacecraft using DARTS. Part 2: Parallel/vectorized real-time implementation

    NASA Technical Reports Server (NTRS)

    Fijany, A.; Roberts, J. A.; Jain, A.; Man, G. K.

    1993-01-01

    Part 1 of this paper presented the requirements for the real-time simulation of Cassini spacecraft along with some discussion of the DARTS algorithm. Here, in Part 2 we discuss the development and implementation of parallel/vectorized DARTS algorithm and architecture for real-time simulation. Development of the fast algorithms and architecture for real-time hardware-in-the-loop simulation of spacecraft dynamics is motivated by the fact that it represents a hard real-time problem, in the sense that the correctness of the simulation depends on both the numerical accuracy and the exact timing of the computation. For a given model fidelity, the computation should be computed within a predefined time period. Further reduction in computation time allows increasing the fidelity of the model (i.e., inclusion of more flexible modes) and the integration routine.

  20. Method for Real-Time Model Based Structural Anomaly Detection

    NASA Technical Reports Server (NTRS)

    Smith, Timothy A. (Inventor); Urnes, James M., Sr. (Inventor); Reichenbach, Eric Y. (Inventor)

    2015-01-01

    A system and methods for real-time model based vehicle structural anomaly detection are disclosed. A real-time measurement corresponding to a location on a vehicle structure during an operation of the vehicle is received, and the real-time measurement is compared to expected operation data for the location to provide a modeling error signal. A statistical significance of the modeling error signal to provide an error significance is calculated, and a persistence of the error significance is determined. A structural anomaly is indicated, if the persistence exceeds a persistence threshold value.

  1. Real Time Target Tracking in a Phantom Using Ultrasonic Imaging

    NASA Astrophysics Data System (ADS)

    Xiao, X.; Corner, G.; Huang, Z.

    In this paper we present a real-time ultrasound image guidance method suitable for tracking the motion of tumors. A 2D ultrasound based motion tracking system was evaluated. A robot was used to control the focused ultrasound and position it at the target that has been segmented from a real-time ultrasound video. Tracking accuracy and precision were investigated using a lesion mimicking phantom. Experiments have been conducted and results show sufficient efficiency of the image guidance algorithm. This work could be developed as the foundation for combining the real time ultrasound imaging tracking and MRI thermometry monitoring non-invasive surgery.

  2. Can Real-Time Data Also Be Climate Quality?

    NASA Astrophysics Data System (ADS)

    Brewer, M.; Wentz, F. J.

    2015-12-01

    GMI, AMSR-2 and WindSat herald a new era of highly accurate and timely microwave data products. Traditionally, there has been a large divide between real-time and re-analysis data products. What if these completely separate processing systems could be merged? Through advanced modeling and physically based algorithms, Remote Sensing Systems (RSS) has narrowed the gap between real-time and research-quality. Satellite microwave ocean products have proven useful for a wide array of timely Earth science applications. Through cloud SST capabilities have enormously benefited tropical cyclone forecasting and day to day fisheries management, to name a few. Oceanic wind vectors enhance operational safety of shipping and recreational boating. Atmospheric rivers are of import to many human endeavors, as are cloud cover and knowledge of precipitation events. Some activities benefit from both climate and real-time operational data used in conjunction. RSS has been consistently improving microwave Earth Science Data Records (ESDRs) for several decades, while making near real-time data publicly available for semi-operational use. These data streams have often been produced in 2 stages: near real-time, followed by research quality final files. Over the years, we have seen this time delay shrink from months or weeks to mere hours. As well, we have seen the quality of near real-time data improve to the point where the distinction starts to blur. We continue to work towards better and faster RFI filtering, adaptive algorithms and improved real-time validation statistics for earlier detection of problems. Can it be possible to produce climate quality data in real-time, and what would the advantages be? We will try to answer these questions…

  3. Real-Time Deposition Monitor for Ultrathin Conductive Films

    NASA Technical Reports Server (NTRS)

    Hines, Jacqueline

    2011-01-01

    continuous, real-time monitoring of film deposition. For use with different films, the device would need to be calibrated to provide an understanding of how film thickness is related to film conductivity, as the device is responding primarily to conductivity effects (and not to mass loading effects) in this ultrathin film regime.

  4. Real-time and reliable human detection in clutter scene

    NASA Astrophysics Data System (ADS)

    Tan, Yumei; Luo, Xiaoshu; Xia, Haiying

    2013-10-01

    To solve the problem that traditional HOG approach for human detection can not achieve real-time detection due to its time-consuming detection, an efficient algorithm based on first segmentation then identify method for real-time human detection is proposed to achieve real-time human detection in clutter scene. Firstly, the ViBe algorithm is used to segment all possible human target regions quickly, and more accurate moving objects is obtained by using the YUV color space to eliminate the shadow; secondly, using the body geometry knowledge can help to found the valid human areas by screening the regions of interest; finally, linear support vector machine (SVM) classifier and HOG are applied to train for human body classifier, to achieve accurate positioning of human body's locations. The results of our comparative experiments demonstrated that the approach proposed can obtain high accuracy, good real-time performance and strong robustness.

  5. Real Time & Power Efficient Adaptive - Robust Control

    NASA Astrophysics Data System (ADS)

    Ioan Gliga, Lavinius; Constantin Mihai, Cosmin; Lupu, Ciprian; Popescu, Dumitru

    2017-01-01

    A design procedure for a control system suited for dynamic variable processes is presented in this paper. The proposed adaptive - robust control strategy considers both adaptive control advantages and robust control benefits. It estimates the degradation of the system’s performances due to the dynamic variation in the process and it then utilizes it to determine when the system must be adapted with a redesign of the robust controller. A single integral criterion is used for the identification of the process, and for the design of the control algorithm, which is expressed in direct form, through a cost function defined in the space of the parameters of both the process and the controller. For the minimization of this nonlinear function, an adequate mathematical programming minimization method is used. The theoretical approach presented in this paper was validated for a closed loop control system, simulated in an application developed in C. Because of the reduced number of operations, this method is suitable for implementation on fast processes. Due to its effectiveness, it increases the idle time of the CPU, thereby saving electrical energy.

  6. Nonlinear real-time optical signal processing

    NASA Astrophysics Data System (ADS)

    Sawchuk, A. A.; Jenkins, B. J.

    1986-07-01

    During the period 1 July 1984 - 30 June 1985, the research under Grant AFOSR-84-0181 has concentrated on four major areas. First, work has continued on an experimental sequential optical binary parallel architecture that is constructed from an array of binary optical switching elements (NOR gates) with interconnections done by a computer-generated hologram. We are examining new binary array SLM's, high efficiency, high space-bandwidth product (SBWP) interconnection holograms, and compact reflection versions of the general architecture with the intent of building a larger demonstration system with great capabilities. Next, we have studied improved methods of providing the interconnections in these systems by the use of hybrid digital/analog (facet) holograms. We have examined analytical techniques for mapping circuit diagrams into gate locations and hologram arrays, and optimization procedures to determine the minimum set of necessary space-invariant basis functions and minimum set of space-variant indexing holograms. Another area of study has been the evaluation of devices and materials for high speed optical switching and bistability. Switching energies of 1 to 10 pJ and response times of 10 ns have been experimentally demonstrated at the University of Arizona for devices consisting of an array of Fabry-Perot cavities filled with a nonlinear material. We have begun to use the specifications of these devices and other high speed switching technologies in order to determine better designs and fundamental limits of the binary optical computing architectures under consideration.

  7. Near real-time skin deformation mapping

    NASA Astrophysics Data System (ADS)

    Kacenjar, Steve; Chen, Suzie; Jafri, Madiha; Wall, Brian; Pedersen, Richard; Bezozo, Richard

    2013-02-01

    A novel in vivo approach is described that provides large area mapping of the mechanical properties of the skin in human patients. Such information is important in the understanding of skin health, cosmetic surgery[1], aging, and impacts of sun exposure. Currently, several methods have been developed to estimate the local biomechanical properties of the skin, including the use of a physical biopsy of local areas of the skin (in vitro methods) [2, 3, and 4], and also the use of non-invasive methods (in vivo) [5, 6, and 7]. All such methods examine localized areas of the skin. Our approach examines the local elastic properties via the generation of field displacement maps of the skin created using time-sequence imaging [9] with 2D digital imaging correlation (DIC) [10]. In this approach, large areas of the skin are reviewed rapidly, and skin displacement maps are generated showing the contour maps of skin deformation. These maps are then used to precisely register skin images for purposes of diagnostic comparison. This paper reports on our mapping and registration approach, and demonstrates its ability to accurately measure the skin deformation through a described nulling interpolation process. The result of local translational DIC alignment is compared using this interpolation process. The effectiveness of the approach is reported in terms of residual RMS, image entropy measures, and differential segmented regional errors.

  8. Real time viability detection of bacterial spores

    DOEpatents

    Vanderberg, Laura A.; Herdendorf, Timothy J.; Obiso, Richard J.

    2003-07-29

    This invention relates to a process for detecting the presence of viable bacterial spores in a sample and to a spore detection system, the process including placing a sample in a germination medium for a period of time sufficient for commitment of any present viable bacterial spores to occur, mixing the sample with a solution of a lanthanide capable of forming a fluorescent complex with dipicolinic acid, and, measuring the sample for the presence of dipicolinic acid, and the system including a germination chamber having inlets from a sample chamber, a germinant chamber and a bleach chamber, the germination chamber further including an outlet through a filtering means, the outlet connected to a detection chamber, the detection chamber having an inlet from a fluorescence promoting metal chamber and the detection chamber including a spectral excitation source and a means of measuring emission spectra from a sample, the detection chamber further connected to a waste chamber. A germination reaction mixture useful for promoting commitment of any viable bacterial spores in a sample including a combination of L-alanine, L-asparagine and D-glucose is also described.

  9. Real-time feedback from iterative electronic structure calculations.

    PubMed

    Vaucher, Alain C; Haag, Moritz P; Reiher, Markus

    2016-04-05

    Real-time feedback from iterative electronic structure calculations requires to mediate between the inherently unpredictable execution times of the iterative algorithm used and the necessity to provide data in fixed and short time intervals for real-time rendering. We introduce the concept of a mediator as a component able to deal with infrequent and unpredictable reference data to generate reliable feedback. In the context of real-time quantum chemistry, the mediator takes the form of a surrogate potential that has the same local shape as the first-principles potential and can be evaluated efficiently to deliver atomic forces as real-time feedback. The surrogate potential is updated continuously by electronic structure calculations and guarantees to provide a reliable response to the operator for any molecular structure. To demonstrate the application of iterative electronic structure methods in real-time reactivity exploration, we implement self-consistent semiempirical methods as the data source and apply the surrogate-potential mediator to deliver reliable real-time feedback.

  10. Real-time estimation of dynamic functional connectivity networks.

    PubMed

    Monti, Ricardo Pio; Lorenz, Romy; Braga, Rodrigo M; Anagnostopoulos, Christoforos; Leech, Robert; Montana, Giovanni

    2017-01-01

    Two novel and exciting avenues of neuroscientific research involve the study of task-driven dynamic reconfigurations of functional connectivity networks and the study of functional connectivity in real-time. While the former is a well-established field within neuroscience and has received considerable attention in recent years, the latter remains in its infancy. To date, the vast majority of real-time fMRI studies have focused on a single brain region at a time. This is due in part to the many challenges faced when estimating dynamic functional connectivity networks in real-time. In this work, we propose a novel methodology with which to accurately track changes in time-varying functional connectivity networks in real-time. The proposed method is shown to perform competitively when compared to state-of-the-art offline algorithms using both synthetic as well as real-time fMRI data. The proposed method is applied to motor task data from the Human Connectome Project as well as to data obtained from a visuospatial attention task. We demonstrate that the algorithm is able to accurately estimate task-related changes in network structure in real-time. Hum Brain Mapp 38:202-220, 2017. © 2016 Wiley Periodicals, Inc.

  11. The First Real-Time Tsunami Animation

    NASA Astrophysics Data System (ADS)

    Becker, N. C.; Wang, D.; McCreery, C.; Weinstein, S.; Ward, B.

    2014-12-01

    For the first time a U.S. tsunami warning center created and issued a tsunami forecast model animation while the tsunami was still crossing an ocean. Pacific Tsunami Warning Center (PTWC) scientists had predicted they would have this ability (Becker et al., 2012) with their RIFT forecast model (Wang et al., 2009) by using rapidly-determined W-phase centroid-moment tensor earthquake focal mechanisms as tsunami sources in the RIFT model (Wang et al., 2012). PTWC then acquired its own YouTube channel in 2013 for its outreach efforts that showed animations of historic tsunamis (Becker et al., 2013), but could also be a platform for sharing future tsunami animations. The 8.2 Mw earthquake of 1 April 2014 prompted PTWC to issue official warnings for a dangerous tsunami in Chile, Peru and Ecuador. PTWC ended these warnings five hours later, then issued its new tsunami marine hazard product (i.e., no coastal evacuations) for the State of Hawaii. With the international warning canceled but with a domestic hazard still present PTWC generated a forecast model animation and uploaded it to its YouTube channel six hours before the arrival of the first waves in Hawaii. PTWC also gave copies of this animation to television reporters who in turn passed it on to their national broadcast networks. PTWC then created a version for NOAA's Science on a Sphere system so it could be shown on these exhibits as the tsunami was still crossing the Pacific Ocean. While it is difficult to determine how many people saw this animation since local, national, and international news networks showed it in their broadcasts, PTWC's YouTube channel provides some statistics. As of 1 August 2014 this animation has garnered more than 650,000 views. Previous animations, typically released during significant anniversaries, rarely get more than 10,000 views, and even then only when external websites share them. Clearly there is a high demand for a tsunami graphic that shows both the speed and the severity of a

  12. ARTSN: An Automated Real-Time Spacecraft Navigation System

    NASA Technical Reports Server (NTRS)

    Burkhart, P. Daniel; Pollmeier, Vincent M.

    1996-01-01

    As part of the Deep Space Network (DSN) advanced technology program an effort is underway to design a filter to automate the deep space navigation process.The automated real-time spacecraft navigation (ARTSN) filter task is based on a prototype consisting of a FORTRAN77 package operating on an HP-9000/700 workstation running HP-UX 9.05. This will be converted to C, and maintained as the operational version. The processing tasks required are: (1) read a measurement, (2) integrate the spacecraft state to the current measurement time, (3) compute the observable based on the integrated state, and (4) incorporate the measurement information into the state using an extended Kalman filter. This filter processes radiometric data collected by the DSN. The dynamic (force) models currently include point mass gravitational terms for all planets, the Sun and Moon, solar radiation pressure, finite maneuvers, and attitude maintenance activity modeled quadratically. In addition, observable errors due to troposphere are included. Further data types, force and observable models will be ncluded to enhance the accuracy of the models and the capability of the package. The heart of the ARSTSN is a currently available continuous-discrete extended Kalman filter. Simulated data used to test the implementation at various stages of development and the results from processing actual mission data are presented.

  13. Developing infrared array controller with software real time operating system

    NASA Astrophysics Data System (ADS)

    Sako, Shigeyuki; Miyata, Takashi; Nakamura, Tomohiko; Motohara, Kentaro; Uchimoto, Yuka Katsuno; Onaka, Takashi; Kataza, Hirokazu

    2008-07-01

    Real-time capabilities are required for a controller of a large format array to reduce a dead-time attributed by readout and data transfer. The real-time processing has been achieved by dedicated processors including DSP, CPLD, and FPGA devices. However, the dedicated processors have problems with memory resources, inflexibility, and high cost. Meanwhile, a recent PC has sufficient resources of CPUs and memories to control the infrared array and to process a large amount of frame data in real-time. In this study, we have developed an infrared array controller with a software real-time operating system (RTOS) instead of the dedicated processors. A Linux PC equipped with a RTAI extension and a dual-core CPU is used as a main computer, and one of the CPU cores is allocated to the real-time processing. A digital I/O board with DMA functions is used for an I/O interface. The signal-processing cores are integrated in the OS kernel as a real-time driver module, which is composed of two virtual devices of the clock processor and the frame processor tasks. The array controller with the RTOS realizes complicated operations easily, flexibly, and at a low cost.

  14. Decision graphs: a tool for developing real-time software

    SciTech Connect

    Kozubal, A.J.

    1981-01-01

    The use of decision graphs in the preparation of, in particular, real-time software is briefly described. The usefulness of decision graphs in software design, testing, and maintenance is pointed out. 2 figures. (RWR)

  15. Near-Real-Time and Laboratory Data by State

    EPA Pesticide Factsheets

    RadNet data from individual near-real-time air monitors is available on a clickable map and is also listed by state and city. Sampling locations for drinking water, precipitation and air filters are also listed.

  16. Intelligent data management for real-time spacecraft monitoring

    NASA Technical Reports Server (NTRS)

    Schwuttke, Ursula M.; Gasser, Les; Abramson, Bruce

    1992-01-01

    Real-time AI systems have begun to address the challenge of restructuring problem solving to meet real-time constraints by making key trade-offs that pursue less than optimal strategies with minimal impact on system goals. Several approaches for adapting to dynamic changes in system operating conditions are known. However, simultaneously adapting system decision criteria in a principled way has been difficult. Towards this end, a general technique for dynamically making such trade-offs using a combination of decision theory and domain knowledge has been developed. Multi-attribute utility theory (MAUT), a decision theoretic approach for making one-time decisions is discussed and dynamic trade-off evaluation is described as a knowledge-based extension of MAUT that is suitable for highly dynamic real-time environments, and provides an example of dynamic trade-off evaluation applied to a specific data management trade-off in a real-world spacecraft monitoring application.

  17. Real-time interactive speech technology at Threshold Technology, Incorporated

    NASA Technical Reports Server (NTRS)

    Herscher, Marvin B.

    1977-01-01

    Basic real-time isolated-word recognition techniques are reviewed. Industrial applications of voice technology are described in chronological order of their development. Future research efforts are also discussed.

  18. Challenges of AVHRR Vegetation Data for Real Time Applications

    NASA Technical Reports Server (NTRS)

    Brown, Molly

    2008-01-01

    Remote sensing data has long been used to monitor global ecosystems for floods and droughts and AVHRR data, as one of the first product, has many users interested in receiving the data within hours of acquisition. With the introduction of a new series of sensors in 2000 (the AVHRR/3 series), the quality of the NDVI datasets available for real time environmental monitoring has declined. This paper provides evidence of problems of cloud contamination, calibration and noise in the real time data which are not present in the historical AVHRR NDVIg dataset. These differences introduce significant uncertainty in the use of the real time data, degrading their utility for detecting climate variations in near real time.

  19. Real-Time Engineering Simulation of Lunar Excavation

    SciTech Connect

    Bruce Damer, George Tompkins, Sheldon Freid, Dave Rasmussen, Peter Newman, Brad Blair

    2007-06-12

    DigitalSpace Corporation has been building an open source real-time three-dimensional (3-D) collaborative design engineering and training platform called Digital Spaces (DSS) in support of the Exploration Vision of the National Aeronautics and Space Administration (NASA). Real-time 3-D simulation has reached a level of maturity where it is capable of supporting engineering design and operations using off-the-shelf game chipsets and open source physics and rendering technologies. This paper will illustrate a state-of-the-art real-time engineering simulation utilizing DSS in support of NASA lunar excavation studies. During the project DigitalSpace building driveable 3-D models of lunar excavators and South Polar terrain, and added a soil mechanics physics model as well as a random failure generator to the repertoire of standard mobility platform physics in prior use for real-time engineering and operational analysis at NASA.

  20. Real-time earthquake monitoring: Early warning and rapid response

    NASA Technical Reports Server (NTRS)

    1991-01-01

    A panel was established to investigate the subject of real-time earthquake monitoring (RTEM) and suggest recommendations on the feasibility of using a real-time earthquake warning system to mitigate earthquake damage in regions of the United States. The findings of the investigation and the related recommendations are described in this report. A brief review of existing real-time seismic systems is presented with particular emphasis given to the current California seismic networks. Specific applications of a real-time monitoring system are discussed along with issues related to system deployment and technical feasibility. In addition, several non-technical considerations are addressed including cost-benefit analysis, public perceptions, safety, and liability.

  1. Application of real time PCR for diagnosis of Swine Dysentery.

    PubMed

    Akase, Satoru; Uchitani, Yumi; Sohmura, Yoshiko; Tatsuta, Keikichi; Sadamasu, Kenji; Adachi, Yoshikazu

    2009-03-01

    Evaluation of a genetic diagnostic technique using real time PCR of Swine Dysentery (SD) was performed using nox primers. Culture, ordinary PCR and real time PCR were compared in this experiment. Sixty-seven specimens from pigs with clinical signs of SD brought to a slaughterhouse in Shibaura, Tokyo, were used. B. hyodysenteriae was isolated from 49 of the pigs, was detected by ordinary PCR in 49 of the pigs and was detected by real time PCR in 54 of the pigs. Furthermore, we were able to determine the numbers of B. hyodysenteriae cells in all positive specimens by real time PCR. The rapid diagnostic technique established in this experiment was useful for detection of B. hyodysenteriae because it was more effective than ordinary PCR and culture.

  2. Cluster Computing for Embedded/Real-Time Systems

    NASA Technical Reports Server (NTRS)

    Katz, D.; Kepner, J.

    1999-01-01

    Embedded and real-time systems, like other computing systems, seek to maximize computing power for a given price, and thus can significantly benefit from the advancing capabilities of cluster computing.

  3. The Effects of Real-Time Interactive Multimedia Teleradiology System

    PubMed Central

    Al-Safadi, Lilac

    2016-01-01

    This study describes the design of a real-time interactive multimedia teleradiology system and assesses how the system is used by referring physicians in point-of-care situations and supports or hinders aspects of physician-radiologist interaction. We developed a real-time multimedia teleradiology management system that automates the transfer of images and radiologists' reports and surveyed physicians to triangulate the findings and to verify the realism and results of the experiment. The web-based survey was delivered to 150 physicians from a range of specialties. The survey was completed by 72% of physicians. Data showed a correlation between rich interactivity, satisfaction, and effectiveness. The results of our experiments suggest that real-time multimedia teleradiology systems are valued by referring physicians and may have the potential for enhancing their practice and improving patient care and highlight the critical role of multimedia technologies to provide real-time multimode interactivity in current medical care. PMID:27294118

  4. Real-time water quality monitoring and providing water quality ...

    EPA Pesticide Factsheets

    EPA and the U.S. Geological Survey (USGS) have initiated the “Village Blue” research project to provide real-time water quality monitoring data to the Baltimore community and increase public awareness about local water quality in Baltimore Harbor and the Chesapeake Bay. The Village Blue demonstration project complements work that a number of state and local organizations are doing to make Baltimore Harbor “swimmable and fishable” 2 by 2020. Village Blue is designed to build upon EPA’s “Village Green” project which provides real-time air quality information to communities in six locations across the country. The presentation, “Real-time water quality monitoring and providing water quality information to the Baltimore Community”, summarizes the Village Blue real-time water quality monitoring project being developed for the Baltimore Harbor.

  5. Real-Time Collision Avoidance for Dexterous 7-DOF Arms

    NASA Technical Reports Server (NTRS)

    Bon, Bruce; Seraji, Homayoun

    1996-01-01

    A new approach to real-time collison avoidance for dexterous 7-DOF arms and supportive simulation and experimental results are presented. The collision avoidance problem is formulated and solved as a force control problem.

  6. Prototype real-time baseband signal combiner. [deep space network

    NASA Technical Reports Server (NTRS)

    Howard, L. D.

    1980-01-01

    The design and performance of a prototype real-time baseband signal combiner, used to enhance the received Voyager 2 spacecraft signals during the Jupiter flyby, is described. Hardware delay paths, operating programs, and firmware are discussed.

  7. Hard-real-time resource management for autonomous spacecraft

    NASA Technical Reports Server (NTRS)

    Gat, E.

    2000-01-01

    This paper describes tickets, a computational mechanism for hard-real-time autonomous resource management. Autonomous spacecraftcontrol can be considered abstractly as a computational process whose outputs are spacecraft commands.

  8. Real-time Position Control of Concentric Tube Robots.

    PubMed

    Dupont, Pierre E; Lock, Jesse; Itkowitz, Brandon

    2010-05-03

    A novel approach to constructing robots is based on concentrically combining pre-curved elastic tubes. By rotating and extending the tubes with respect to each other, their curvatures interact elastically to position and orient the robot's tip, as well as to control the robot's shape along its length. Since these robots form slender curves, they are well suited for minimally invasive medical procedures. A substantial challenge to their practical use is the real-time solution of their kinematics that are described by differential equations with split boundary equations. This paper proposes a numerically efficient approach to real-time position control. It is shown that the forward kinematics are smooth functions that can be pre-computed and accurately approximated using Fourier series. The inverse kinematics can be solved in real time using root finding applied to the functional approximation. Experimental demonstration of real-time position control using this approach is also described.

  9. The Effects of Real-Time Interactive Multimedia Teleradiology System.

    PubMed

    Al-Safadi, Lilac

    2016-01-01

    This study describes the design of a real-time interactive multimedia teleradiology system and assesses how the system is used by referring physicians in point-of-care situations and supports or hinders aspects of physician-radiologist interaction. We developed a real-time multimedia teleradiology management system that automates the transfer of images and radiologists' reports and surveyed physicians to triangulate the findings and to verify the realism and results of the experiment. The web-based survey was delivered to 150 physicians from a range of specialties. The survey was completed by 72% of physicians. Data showed a correlation between rich interactivity, satisfaction, and effectiveness. The results of our experiments suggest that real-time multimedia teleradiology systems are valued by referring physicians and may have the potential for enhancing their practice and improving patient care and highlight the critical role of multimedia technologies to provide real-time multimode interactivity in current medical care.

  10. Detailed gas and diesel vehicle emissions: PTR-MS measurements of real-time VOC profiles and comprehensive characterization of primary emissions for IVOC, SVOC, and LVOC by gas chromatography with vacuum ultra-violet ionization mass spectrometry

    NASA Astrophysics Data System (ADS)

    Drozd, G.; Frodin, B.; Zhao, Y.; Franklin, J. P.; Cross, E. S.; Saleh, R.; Saliba, G.; Lambe, A. T.; Sardar, S.; Maldonado, H.; Russell, L. M.; Kroll, J. H.; Robinson, A. L.; Goldstein, A. H.

    2015-12-01

    Over the past fifteen years US vehicle emissions standards have dramatically improved, with the goal of reducing urban air pollution. Recent studies demonstrate secondary organic aerosol (SOA) to be the dominant contributor to urban organic aerosol, but controversy remains regarding the contributions of different vehicle types to SOA. Increased potency for SOA formation from non methane hydrocarbons (NMHC) from newer vehicles that meet tighter emission standards has also been observed. Both speciation and temporal resolution of vehicular emissions are critical for predicting SOA formation. The relative importance of diesel and gasoline emissions to SOA formation depends critically on speciation. Experiments were conducted at the California Air Resources Board Haagen-Smit Laboratory to better understand SOA formation for low, ultra-low, super ultra-low and partial zero emission vehicles (LEV, ULEV, SULEV, PZEV). Exhaust was sampled on filters and adsorbent tubes to measure intermediate-, semi-, and low-volatility NMHC (IVOC, SVOC, LVOC). A proton-transfer-reaction mass spectrometer (PTR-MS) measured volatile organics (VOC) emissions with high time-resolution. Analysis of filters and adsorbent tubes using gas chromatography with vacuum-ultra-violet ionization mass spectrometry provided unprecedented characterization of emissions according to degree of branching, number of cyclic rings, aromaticity, and molecular weight. ULEV vehicles show the composition distributions of primary particulate emissions peak for compounds in the SVOC range. PZEV vehicle emissions peak in the IVOC range. Diesel vehicles have up to ten times higher emissions than gasoline vehicles; their distributions have significant IVOC levels and peak in the SVOC/LVOC range. Our measurements are used to predict potential SOA formation by vehicle standard class and the relative SOA formation for diesel and gasoline vehicles. PTR-MS measurement show VOC emissions after cold start occur almost entirely

  11. Evaluation of Open-Source Hard Real Time Software Packages

    NASA Technical Reports Server (NTRS)

    Mattei, Nicholas S.

    2004-01-01

    Reliable software is, at times, hard to find. No piece of software can be guaranteed to work in every situation that may arise during its use here at Glenn Research Center or in space. The job of the Software Assurance (SA) group in the Risk Management Office is to rigorously test the software in an effort to ensure it matches the contract specifications. In some cases the SA team also researches new alternatives for selected software packages. This testing and research is an integral part of the department of Safety and Mission Assurance. Real Time operation in reference to a computer system is a particular style of handing the timing and manner with which inputs and outputs are handled. A real time system executes these commands and appropriate processing within a defined timing constraint. Within this definition there are two other classifications of real time systems: hard and soft. A soft real time system is one in which if the particular timing constraints are not rigidly met there will be no critical results. On the other hand, a hard real time system is one in which if the timing constraints are not met the results could be catastrophic. An example of a soft real time system is a DVD decoder. If the particular piece of data from the input is not decoded and displayed to the screen at exactly the correct moment nothing critical will become of it, the user may not even notice it. However, a hard real time system is needed to control the timing of fuel injections or steering on the Space Shuttle; a delay of even a fraction of a second could be catastrophic in such a complex system. The current real time system employed by most NASA projects is Wind River's VxWorks operating system. This is a proprietary operating system that can be configured to work with many of NASA s needs and it provides very accurate and reliable hard real time performance. The down side is that since it is a proprietary operating system it is also costly to implement. The prospect of

  12. Real-time flight test data distribution and display

    NASA Technical Reports Server (NTRS)

    Nesel, Michael C.; Hammons, Kevin R.

    1988-01-01

    Enhancements to the real-time processing and display systems of the NASA Western Aeronautical Test Range are described. Display processing has been moved out of the telemetry and radar acquisition processing systems super-minicomputers into user/client interactive graphic workstations. Real-time data is provided to the workstations by way of Ethernet. Future enhancement plans include use of fiber optic cable to replace the Ethernet.

  13. Final report on Arete's real-time processor

    SciTech Connect

    Sanborn, J.; Reder, L.; Tong, K. )

    1990-01-12

    This is Arete Associates' final report on the initial phase of a project to develop a real-time processor for an airborne lidar system. Arete has developed a programmable Real-Time Processor (RTP) and image display and recording system. It is presently integrated with an ocean (water) lidar sensor (OWLS) system onboard one of the Navel Air Development Center's (NADC's) P-3A aircraft. The RTP is a rack mounted, PF/AT based system.

  14. Near real-time stereo matching using geodesic diffusion.

    PubMed

    De-Maeztu, Leonardo; Villanueva, Arantxa; Cabeza, Rafael

    2012-02-01

    Adaptive-weight algorithms currently represent the state of the art in local stereo matching. However, due to their computational requirements, these types of solutions are not suitable for real-time implementation. Here, we present a novel aggregation method inspired by the anisotropic diffusion technique used in image filtering. The proposed aggregation algorithm produces results similar to adaptive-weight solutions while reducing the computational requirements. Moreover, near real-time performance is demonstrated with a GPU implementation of the algorithm.

  15. Real-time shipboard orbit determination using Kalman filtering techniques

    NASA Technical Reports Server (NTRS)

    Brammer, R. F.

    1974-01-01

    The real-time tracking and orbit determination program used on board the NASA tracking ship, the USNS Vanguard, is described in this paper. The computer program uses a variety of filtering algorithms, including an extended Kalman filter, to derive real-time orbit determinations (position-velocity state vectors) from shipboard tracking and navigation data. Results from Apollo missions are given to show that orbital parameters can be estimated quickly and accurately using these methods.

  16. Evaluation Criteria for Real-Time Specification Languages

    DTIC Science & Technology

    1992-02-11

    languages designed to specify the requirements of real-time systems. It is intended for a reader who is beginning a real-time development project and... project staffed with unskilled or inexperienced personnel than one with seasoned veterans; sophisticated support tools may be irrelevant to a project ...motivate the project manager to think about long-term issues and provide a justification framework for choosing a particular language and rejecting

  17. High speed, real-time, camera bandwidth converter

    DOEpatents

    Bower, Dan E; Bloom, David A; Curry, James R

    2014-10-21

    Image data from a CMOS sensor with 10 bit resolution is reformatted in real time to allow the data to stream through communications equipment that is designed to transport data with 8 bit resolution. The incoming image data has 10 bit resolution. The communication equipment can transport image data with 8 bit resolution. Image data with 10 bit resolution is transmitted in real-time, without a frame delay, through the communication equipment by reformatting the image data.

  18. Real-time laser holographic Interferometry for aerodynamics

    NASA Technical Reports Server (NTRS)

    Lee, George

    1987-01-01

    Recent developments in thermoplastic recording holograms and advancements in automated image digitalization and analysis make real-time laser holographic interferometry feasible for two-dimensional flows such as airfoil flows. Typical airfoil measurements would include airfoil presssure distributions, wake and boundary layer profiles, and flow field density contours. This paper addresses some of the problems and requirements of a real-time laser holographic interferometer.

  19. Real-time laser holographic interferometry for aerodynamics

    NASA Technical Reports Server (NTRS)

    Lee, George

    1987-01-01

    Recent developments in thermoplastic recording holograms and advancements in automated image digitalization and analysis make real-time laser holographic interferometry feasible for two-dimensional flows such as airfoil flows. Typical airfoil measurements would include airfoil pressure distributions, wake and boundary layer profiles, and flow field density contours. This paper addresses some of the problems and requirements of a real-time laser holographic interferometer.

  20. Improving Predictability in Embedded Real-Time Systems

    DTIC Science & Technology

    2000-12-01

    Systems CMU/SEI-2000-SR-011 Peter H. Feiler , Software Engineering Institute Bruce Lewis, U.S. Army Aviation and Missile Command Steve Vestal...SUBTITLE Improving Predictability in Embedded Real-Time Systems 5. FUNDING NUMBERS F19628-00-C-0003 6. AUTHOR(S) Peter H. Feiler , Bruce ...Carnegie Metton Software Engineering Institute Improving Predictability in Embedded Real-Time Systems Peter H. Feiler , Software Engineering

  1. Real-Time Optical Correlator Based On GaAs

    NASA Technical Reports Server (NTRS)

    Liu, Tsuen-Hsi; Cheng, Li-Jen

    1992-01-01

    Apparatus performs correlation between input image and reference image in real time by means of degenerate four-wave mixing in photorefractive crystal, which serves as real-time holographic medium. Gallium arsenide chosen to be photorefractive material in this application because at frame rate and level of illumination used in experiments, offers adequate diffraction efficiency. Frame rates as high as 1,000 s to negative 1st power achievable.

  2. Real-time Java for on-board systems

    NASA Astrophysics Data System (ADS)

    Cechticky, V.; Pasetti, A.

    2002-07-01

    The Java language has several attractive features but cannot at present be used in on-board systems primarily because it lacks support for hard real-time operation. This shortcoming is in being addressed: some suppliers are already providing implementations of Java that are RT-compliant; Sun Microsystem has approved a formal specification for a real-time extension of the language; and an independent consortium is working on an alternative specification for real-time Java. It is therefore expected that, within a year or so, standardized commercial implementations of real-time Java will be on the market. Availability of real-time implementations now opens the way to its use on-board. Within this context, this paper has two objectives. Firstly, it discusses the suitability of Java for on-board applications. Secondly, it reports the results of an ESA study to port a software framework for on-board control systems to a commercial real-time version of Java.

  3. Real time magnetic resonance guided endomyocardial local delivery

    PubMed Central

    Corti, R; Badimon, J; Mizsei, G; Macaluso, F; Lee, M; Licato, P; Viles-Gonzalez, J F; Fuster, V; Sherman, W

    2005-01-01

    Objective: To investigate the feasibility of targeting various areas of left ventricle myocardium under real time magnetic resonance (MR) imaging with a customised injection catheter equipped with a miniaturised coil. Design: A needle injection catheter with a mounted resonant solenoid circuit (coil) at its tip was designed and constructed. A 1.5 T MR scanner with customised real time sequence combined with in-room scan running capabilities was used. With this system, various myocardial areas within the left ventricle were targeted and injected with a gadolinium-diethylenetriaminepentaacetic acid (DTPA) and Indian ink mixture. Results: Real time sequencing at 10 frames/s allowed clear visualisation of the moving catheter and its transit through the aorta into the ventricle, as well as targeting of all ventricle wall segments without further image enhancement techniques. All injections were visualised by real time MR imaging and verified by gross pathology. Conclusion: The tracking device allowed real time in vivo visualisation of catheters in the aorta and left ventricle as well as precise targeting of myocardial areas. The use of this real time catheter tracking may enable precise and adequate delivery of agents for tissue regeneration. PMID:15710717

  4. Real-time airborne hyperspectral imaging of land mines

    NASA Astrophysics Data System (ADS)

    Ivanco, Tyler; Achal, Steve; McFee, John E.; Anger, Cliff; Young, Jane

    2007-04-01

    DRDC Suffeld and Itres Research have jointly investigated the use of visible and infrared hyperspectral imaging (HSI) for surface and buried land mine detection since 1989. These studies have demonstrated reliable passive HSI detection of surface-laid mines, based on their reflectance spectra, from airborne and ground-based platforms. Commercial HSI instruments collect and store image data at aircraft speeds, but the data are analysed off- line. This is useful for humanitarian demining, but unacceptable for military countermine operations. We have developed a hardware and software system with algorithms that can process the raw hyperspectral data in real time to detect mines. The custom algorithms perform radiometric correction of the raw data, then classify pixels of the corrected data, referencing a spectral signature library. The classification results are stored and displayed in real time, that is, within a few frame times of the data acquisition. Such real-time mine detection was demonstrated for the first time from a slowly moving land vehicle in March 2000. This paper describes an improved system which can achieve real-time detection of mines from an airborne platform, with its commensurately higher data rates. The system is presently compatible with the Itres family of visible/near infrared, short wave infrared and thermal infrared pushbroom hyperspectral imagers and its broadband thermal infrared pushbroom imager. Experiments to detect mines from an airborne platform in real time were conducted at DRDC Suffield in November 2006. Surface-laid land mines were detected in real time from a slowly moving helicopter with generally good detection rates and low false alarm rates. To the authors' knowledge, this is the first time that land mines have been detected from an airborne platform in real time using hyperspectral imaging.

  5. A portable real-time ultrasonic bone densitometer.

    PubMed

    Kaufman, Jonathan J; Luo, Gangming; Siffert, Robert S

    2007-09-01

    The objectives of this study were to develop a novel ultrasound device to estimate bone mineral density (BMD) at the calcaneus. The device is entirely self-contained, portable (real-time evaluation of the BMD by computing a parameter known as net time delay (NTD). The NTD is defined as the difference between the transit time through the heel of an ultrasound signal and the transit time through a hypothetical object of equal thickness (to the heel) but containing soft tissue only. This parameter is sensitive primarily to the total amount (i.e., the average total thickness) of bone contained in the propagation path, and thus is equivalent to the bone mineral content estimated by dual-energy X-ray absorptiometry (DXA) scanners, and to the (areal) BMD when normalized by transducer area. Computer simulations of ultrasound propagation were used to study the relationship between NTD and BMD. The simulations used micro-computed tomography (micro-CT) images of a set of 10 calcaneal bone cores, which were further processed by morphologic image processing to obtain a set of 30 "samples" with BMDs ranging from 0.25 to 1.83 g/cm2. The NTD and BMD were found to be very highly correlated (r=0.99), demonstrating the high sensitivity of NTD to bone mass. A clinical institutional review board-approved study measured 85 adult women at the heel. BMD was measured at the same time using DXA. A linear regression using NTD produced a linear correlation coefficient of 0.86, which represents a significant improvement over present ultrasound bone densitometers, but not nearly as good as the simulation results. Reasons for this have been identified (viz., errors in distance measurement and lack of coincidence between the DXA and ultrasound regions of interest), and a new device and experimental protocol to deal with these sources of error has been developed and is currently under clinical trials. It is expected that this should improve the correlation

  6. Real-Time Visualization System for Computational Offloading

    DTIC Science & Technology

    2015-01-01

    Real- Time Visualization System for Computational Offloading by Bryan Dawson and David L Doria ARL-TN-0655 January 2015... Time Visualization System for Computational Offloading Bryan Dawson Oak Ridge Institute for Science and Education David L Doria Computational...hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and

  7. Explaining How to Play Real-Time Strategy Games

    NASA Astrophysics Data System (ADS)

    Metoyer, Ronald; Stumpf, Simone; Neumann, Christoph; Dodge, Jonathan; Cao, Jill; Schnabel, Aaron

    Real-time strategy games share many aspects with real situations in domains such as battle planning, air traffic control, and emergency response team management which makes them appealing test-beds for Artificial Intelligence (AI) and machine learning. End user annotations could help to provide supplemental information for learning algorithms, especially when training data is sparse. This paper presents a formative study to uncover how experienced users explain game play in real-time strategy games. We report the results of our analysis of explanations and discuss their characteristics that could support the design of systems for use by experienced real-time strategy game users in specifying or annotating strategy-oriented behavior.

  8. Real time microcontroller implementation of an adaptive myoelectric filter.

    PubMed

    Bagwell, P J; Chappell, P H

    1995-03-01

    This paper describes a real time digital adaptive filter for processing myoelectric signals. The filter time constant is automatically selected by the adaptation algorithm, giving a significant improvement over linear filters for estimating the muscle force and controlling a prosthetic device. Interference from mains sources often produces problems for myoelectric processing, and so 50 Hz and all harmonic frequencies are reduced by an averaging filter and differential process. This makes practical electrode placement and contact less critical and time consuming. An economic real time implementation is essential for a prosthetic controller, and this is achieved using an Intel 80C196KC microcontroller.

  9. Geomagnetic Observatory Data for Real-Time Applications

    NASA Astrophysics Data System (ADS)

    Love, J. J.; Finn, C. A.; Rigler, E. J.; Kelbert, A.; Bedrosian, P.

    2015-12-01

    The global network of magnetic observatories represents a unique collective asset for the scientific community. Historically, magnetic observatories have supported global magnetic-field mapping projects and fundamental research of the Earth's interior and surrounding space environment. More recently, real-time data streams from magnetic observatories have become an important contributor to multi-sensor, operational monitoring of evolving space weather conditions, especially during magnetic storms. In this context, the U.S. Geological Survey (1) provides real-time observatory data to allied space weather monitoring projects, including those of NOAA, the U.S. Air Force, NASA, several international agencies, and private industry, (2) collaborates with Schlumberger to provide real-time geomagnetic data needed for directional drilling for oil and gas in Alaska, (3) develops products for real-time evaluation of hazards for the electric-power grid industry that are associated with the storm-time induction of geoelectric fields in the Earth's conducting lithosphere. In order to implement strategic priorities established by the USGS Natural Hazards Mission Area and the National Science and Technology Council, and with a focus on developing new real-time products, the USGS is (1) leveraging data management protocols already developed by the USGS Earthquake Program, (2) developing algorithms for mapping geomagnetic activity, a collaboration with NASA and NOAA, (3) supporting magnetotelluric surveys and developing Earth conductivity models, a collaboration with Oregon State University and the NSF's EarthScope Program, (4) studying the use of geomagnetic activity maps and Earth conductivity models for real-time estimation of geoelectric fields, (5) initiating geoelectric monitoring at several observatories, (6) validating real-time estimation algorithms against historical geomagnetic and geoelectric data. The success of these long-term projects is subject to funding constraints

  10. Real-time multi-mode neutron multiplicity counter

    DOEpatents

    Rowland, Mark S; Alvarez, Raymond A

    2013-02-26

    Embodiments are directed to a digital data acquisition method that collects data regarding nuclear fission at high rates and performs real-time preprocessing of large volumes of data into directly useable forms for use in a system that performs non-destructive assaying of nuclear material and assemblies for mass and multiplication of special nuclear material (SNM). Pulses from a multi-detector array are fed in parallel to individual inputs that are tied to individual bits in a digital word. Data is collected by loading a word at the individual bit level in parallel, to reduce the latency associated with current shift-register systems. The word is read at regular intervals, all bits simultaneously, with no manipulation. The word is passed to a number of storage locations for subsequent processing, thereby removing the front-end problem of pulse pileup. The word is used simultaneously in several internal processing schemes that assemble the data in a number of more directly useable forms. The detector includes a multi-mode counter that executes a number of different count algorithms in parallel to determine different attributes of the count data.

  11. Two essays on real-time pricing of electric power

    NASA Astrophysics Data System (ADS)

    Gupta, Nainish Kumar

    1997-09-01

    This dissertation contains two essays on a new innovative pricing mechanism in the electric power industry known as Real Time Pricing (RTP). RTP is a method of pricing electric power wherein at least one component of the price is set at or near levels that reflect the marginal costs of providing power during each time-specific interval. These prices vary in accord with time-specific, incremental supplier costs. RTP allows customers to manage their own bills by adjusting their consumption as spot prices and supplier costs vary, which may amount during a single day from 2 cents to 85 cents for one kilowatt hour (kWh) of usage. Using 1995 data the hypothesis that industrial customers shift their usage patterns of electricity in response to real time prices is tested. To measure customer responsiveness to real time electric rates, econometric techniques are applied to estimate demand elasticities.

  12. Real Time Fire Reconnaissance Satellite Monitoring System Failure Model

    NASA Astrophysics Data System (ADS)

    Nino Prieto, Omar Ariosto; Colmenares Guillen, Luis Enrique

    2013-09-01

    In this paper the Real Time Fire Reconnaissance Satellite Monitoring System is presented. This architecture is a legacy of the Detection System for Real-Time Physical Variables which is undergoing a patent process in Mexico. The methodologies for this design are the Structured Analysis for Real Time (SA- RT) [8], and the software is carried out by LACATRE (Langage d'aide à la Conception d'Application multitâche Temps Réel) [9,10] Real Time formal language. The system failures model is analyzed and the proposal is based on the formal language for the design of critical systems and Risk Assessment; AltaRica. This formal architecture uses satellites as input sensors and it was adapted from the original model which is a design pattern for physical variation detection in Real Time. The original design, whose task is to monitor events such as natural disasters and health related applications, or actual sickness monitoring and prevention, as the Real Time Diabetes Monitoring System, among others. Some related work has been presented on the Mexican Space Agency (AEM) Creation and Consultation Forums (2010-2011), and throughout the International Mexican Aerospace Science and Technology Society (SOMECYTA) international congress held in San Luis Potosí, México (2012). This Architecture will allow a Real Time Fire Satellite Monitoring, which will reduce the damage and danger caused by fires which consumes the forests and tropical forests of Mexico. This new proposal, permits having a new system that impacts on disaster prevention, by combining national and international technologies and cooperation for the benefit of humankind.

  13. Miniaturised Time-of-Flight Mass Spectrometer

    NASA Astrophysics Data System (ADS)

    Rohner, U.; Benz, W.; Whitby, J. A.; Wurz, P.; Schulz, R.; Romstedt, J.

    2004-04-01

    Originally intended for the European Space Agency's BepiColombo mission to Mercury, we have built a series of highly miniaturised laser ablation time of flight mass spectrometers (LMS), suitable for in situ measurements of the elemental and isotopic composition of the surface of airless planetary bodies. The instruments will determine ma jor, minor, and trace element abundances in minerals on a spatial scale of 10 m, and will have sufficient dynamic range and mass resolution to perform useful isotopic measurements in favourable cases. Solid material is simultaneously evaporated and ionised by means of laser ablation, requiring intense pulsed laser radiation. Laser ablation was chosen as the sample introduction technique principally because of its high spatial resolution and the lack of any need for sample preparation. Advantages of the technique include simplicity of the resulting design, speed of measurement, and the ability for depth profiling (potentially important for a regolith in which mineral grains are coated with impact produced glass). Time of flight mass spectrometers are simple, robust devices that couple well to a pulsed ion source and we have previous experience of their construction for space flight, e.g. the ROSINA instrument suite for the ROSETTA mission. For BepiColombo, we have built two prototype instruments, one with a design mass of 500 g and a volume comparable to a beer can intended to be deployed on a static lander, and a smaller cigarettebox sized version with a design mass of 250 g, small enough to be integrated in a rover or robotic arm.

  14. Lightweight distributed computing for intraoperative real-time image guidance

    NASA Astrophysics Data System (ADS)

    Suwelack, Stefan; Katic, Darko; Wagner, Simon; Spengler, Patrick; Bodenstedt, Sebastian; Röhl, Sebastian; Dillmann, Rüdiger; Speidel, Stefanie

    2012-02-01

    In order to provide real-time intraoperative guidance, computer assisted surgery (CAS) systems often rely on computationally expensive algorithms. The real-time constraint is especially challenging if several components such as intraoperative image processing, soft tissue registration or context aware visualization are combined in a single system. In this paper, we present a lightweight approach to distribute the workload over several workstations based on the OpenIGTLink protocol. We use XML-based message passing for remote procedure calls and native types for transferring data such as images, meshes or point coordinates. Two different, but typical scenarios are considered in order to evaluate the performance of the new system. First, we analyze a real-time soft tissue registration algorithm based on a finite element (FE) model. Here, we use the proposed approach to distribute the computational workload between a primary workstation that handles sensor data processing and visualization and a dedicated workstation that runs the real-time FE algorithm. We show that the additional overhead that is introduced by the technique is small compared to the total execution time. Furthermore, the approach is used to speed up a context aware augmented reality based navigation system for dental implant surgery. In this scenario, the additional delay for running the computationally expensive reasoning server on a separate workstation is less than a millisecond. The results show that the presented approach is a promising strategy to speed up real-time CAS systems.

  15. Real-time simulation of thermal shadows with EMIT

    NASA Astrophysics Data System (ADS)

    Klein, Andreas; Oberhofer, Stefan; Schätz, Peter; Nischwitz, Alfred; Obermeier, Paul

    2016-05-01

    Modern missile systems use infrared imaging for tracking or target detection algorithms. The development and validation processes of these missile systems need high fidelity simulations capable of stimulating the sensors in real-time with infrared image sequences from a synthetic 3D environment. The Extensible Multispectral Image Generation Toolset (EMIT) is a modular software library developed at MBDA Germany for the generation of physics-based infrared images in real-time. EMIT is able to render radiance images in full 32-bit floating point precision using state of the art computer graphics cards and advanced shader programs. An important functionality of an infrared image generation toolset is the simulation of thermal shadows as these may cause matching errors in tracking algorithms. However, for real-time simulations, such as hardware in the loop simulations (HWIL) of infrared seekers, thermal shadows are often neglected or precomputed as they require a thermal balance calculation in four-dimensions (3D geometry in one-dimensional time up to several hours in the past). In this paper we will show the novel real-time thermal simulation of EMIT. Our thermal simulation is capable of simulating thermal effects in real-time environments, such as thermal shadows resulting from the occlusion of direct and indirect irradiance. We conclude our paper with the practical use of EMIT in a missile HWIL simulation.

  16. Real-Time Tropospheric Delay Estimation using IGS Products

    NASA Astrophysics Data System (ADS)

    Stürze, Andrea; Liu, Sha; Söhne, Wolfgang

    2014-05-01

    The Federal Agency for Cartography and Geodesy (BKG) routinely provides zenith tropospheric delay (ZTD) parameter for the assimilation in numerical weather models since more than 10 years. Up to now the results flowing into the EUREF Permanent Network (EPN) or E-GVAP (EUMETNET EIG GNSS water vapour programme) analysis are based on batch processing of GPS+GLONASS observations in differential network mode. For the recently started COST Action ES1206 about "Advanced Global Navigation Satellite Systems tropospheric products for monitoring severe weather events and climate" (GNSS4SWEC), however, rapid updates in the analysis of the atmospheric state for nowcasting applications require changing the processing strategy towards real-time. In the RTCM SC104 (Radio Technical Commission for Maritime Services, Special Committee 104) a format combining the advantages of Precise Point Positioning (PPP) and Real-Time Kinematic (RTK) is under development. The so-called State Space Representation approach is defining corrections, which will be transferred in real-time to the user e.g. via NTRIP (Network Transport of RTCM via Internet Protocol). Meanwhile messages for precise orbits, satellite clocks and code biases compatible to the basic PPP mode using IGS products are defined. Consequently, the IGS Real-Time Service (RTS) was launched in 2013 in order to extend the well-known precise orbit and clock products by a real-time component. Further messages e.g. with respect to ionosphere or phase biases are foreseen. Depending on the level of refinement, so different accuracies up to the RTK level shall be reachable. In co-operation of BKG and the Technical University of Darmstadt the real-time software GEMon (GREF EUREF Monitoring) is under development. GEMon is able to process GPS and GLONASS observation and RTS product data streams in PPP mode. Furthermore, several state-of-the-art troposphere models, for example based on numerical weather prediction data, are implemented. Hence, it

  17. A real-time VLC to UART protocol conversion system

    NASA Astrophysics Data System (ADS)

    Deng, Jian-zhi; Yao, Meng; Cheng, Xiao-hui; Deng, Zhuo-hong

    2016-07-01

    A real-time visible light communication (VLC) to universal asynchronous receiver/transmitter (UART) conversion system is made up of a transmitter with a light emitting diode (LED) and a receiver with a photodiode (PD), by which a VLC system is connected to traditional communication modes, and the data are transferred by wireless visible light. UART packets are converted to light packets by the modulation of a 10 kHz on-off-keying (OOK) light signal, and the data losses in the transportation are avoided by the protection of a data buffer mechanism. The experimental results reveal that the real-time VLC to UART conversion system can provide a real-time VLC transmission way for two UART devices in not less than 10 m at a baud rate not less than 19 200 Bd with stable ambient lighting at the same time.

  18. Real-Time Parameter Estimation Using Output Error

    NASA Technical Reports Server (NTRS)

    Grauer, Jared A.

    2014-01-01

    Output-error parameter estimation, normally a post- ight batch technique, was applied to real-time dynamic modeling problems. Variations on the traditional algorithm were investigated with the goal of making the method suitable for operation in real time. Im- plementation recommendations are given that are dependent on the modeling problem of interest. Application to ight test data showed that accurate parameter estimates and un- certainties for the short-period dynamics model were available every 2 s using time domain data, or every 3 s using frequency domain data. The data compatibility problem was also solved in real time, providing corrected sensor measurements every 4 s. If uncertainty corrections for colored residuals are omitted, this rate can be increased to every 0.5 s.

  19. Safe Runtime Verification of Real-Time Properties

    NASA Astrophysics Data System (ADS)

    Colombo, Christian; Pace, Gordon J.; Schneider, Gerardo

    Introducing a monitor on a system typically changes the system’s behaviour by slowing the system down and increasing memory consumption. This may possibly result in creating new bugs, or possibly even ‘fixing’ bugs, only to reappear as the monitor is removed. Properties written in a real-time logic, such as duration calculus, can be particularly sensitive to such changes induced through monitoring. The same problem occurs in other scenarios such as when a system is ported to a faster machine. In this paper, we identify a class of real-time properties, in duration calculus, which are monotonic under the slowing down (speeding up) of the underlying system. We apply this approach to the real-time runtime monitoring tool Larva, where we use duration calculus as a monitoring property specification language, so we automatically identify properties which can be shown to be monotonic with respect to system re-timing.

  20. Modeling and Real-Time Simulation of UPFC

    NASA Astrophysics Data System (ADS)

    Kimura, Misao; Takahashi, Choei; Kishibe, Hideto; Miyazaki, Yasuyuki; Noro, Yasuhiro; Iio, Naotaka

    We have developed a digital real time simulator of Power Electronics Controllers, so called FACTS (Flexible AC Transmission Systems) Controllers and/or Custom Power by using MATLABTM/SIMULINKTM and dSPACETM System. This paper describes the modeling and the calculation accuracy of a UPFC (Unified Power Flow Controller) model. Hence the developed simulator operates at a large time step, in order to improve simulation accuracy, a correction processing of the switching delay is implemented into the UPFC model. Calculation accuracy of the real time UPFC model is the same level as EMTDCTM results. We confirm stable operation of the developed UPFC model with connecting a commercial real time digital simulator (RTDSTM).

  1. Coordinated scheduling for dynamic real-time systems

    NASA Technical Reports Server (NTRS)

    Natarajan, Swaminathan; Zhao, Wei

    1994-01-01

    In this project, we addressed issues in coordinated scheduling for dynamic real-time systems. In particular, we concentrated on design and implementation of a new distributed real-time system called R-Shell. The design objective of R-Shell is to provide computing support for space programs that have large, complex, fault-tolerant distributed real-time applications. In R-shell, the approach is based on the concept of scheduling agents, which reside in the application run-time environment, and are customized to provide just those resource management functions which are needed by the specific application. With this approach, we avoid the need for a sophisticated OS which provides a variety of generalized functionality, while still not burdening application programmers with heavy responsibility for resource management. In this report, we discuss the R-Shell approach, summarize the achievement of the project, and describe a preliminary prototype of R-Shell system.

  2. A framework for building real-time expert systems

    NASA Technical Reports Server (NTRS)

    Lee, S. Daniel

    1991-01-01

    The Space Station Freedom is an example of complex systems that require both traditional and artificial intelligence (AI) real-time methodologies. It was mandated that Ada should be used for all new software development projects. The station also requires distributed processing. Catastrophic failures on the station can cause the transmission system to malfunction for a long period of time, during which ground-based expert systems cannot provide any assistance to the crisis situation on the station. This is even more critical for other NASA projects that would have longer transmission delays (e.g., the lunar base, Mars missions, etc.). To address these issues, a distributed agent architecture (DAA) is proposed that can support a variety of paradigms based on both traditional real-time computing and AI. The proposed testbed for DAA is an autonomous power expert (APEX) which is a real-time monitoring and diagnosis expert system for the electrical power distribution system of the space station.

  3. Miniature Time-of-Flight Mass Spectrometer

    NASA Technical Reports Server (NTRS)

    Potember, Richard S.

    1999-01-01

    Major advances must occur to protect astronauts from prolonged periods in near-zero gravity and high radiation associated with extended space travel. The dangers of living in space must be thoroughly understood and methods developed to reverse those effects that cannot be avoided. Six of the seven research teams established by the National Space Biomedical Research Institute (NSBRI) are studying biomedical factors for prolonged space travel to deliver effective countermeasures. To develop effective countermeasures, each of these teams require identification of and quantitation of complex pharmacological, hormonal, and growth factor compounds (biomarkers) in humans and in experimental animals to develop an in-depth knowledge of the physiological changes associated with space travel. At present, identification of each biomarker requires a separate protocol. Many of these procedures are complicated and the identification of each biomarker requires a separate protocol and associated laboratory equipment. To carry all of this equipment and chemicals on a spacecraft would require a complex clinical laboratory; and it would occupy much of the astronauts time. What is needed is a small, efficient, broadband medical diagnostic instrument to rapidly identify important biomarkers for human space exploration. The Miniature Time-Of- Flight Mass Spectrometer Project in the Technology Development Team is developing a small, high resolution, time-of-flight mass spectrometer (TOFMS) to quantitatively measure biomarkers for human space exploration. Virtues of the JHU/APL TOFMS technologies reside in the promise for a small (less than one cubic ft), lightweight (less than 5 kg), low-power (less than 50 watts), rugged device that can be used continuously with advanced signal processing diagnostics. To date, the JHU/APL program has demonstrated mass capability from under 100 to beyond 10,000 atomic mass units (amu) in a very small, low power prototype for biological analysis. Further

  4. Real-time alerts and reminders using information systems.

    PubMed

    Wanderer, Jonathan P; Sandberg, Warren S; Ehrenfeld, Jesse M

    2011-09-01

    Adoption of information systems throughout the hospital environment has enabled the development of real-time physiologic alerts and clinician reminder systems. These clinical tools can be made available through the deployment of anesthesia information management systems (AIMS). Creating usable alert systems requires understanding of technical considerations. Various successful implementations are reviewed, encompassing cost reduction, improved revenue capture, timely antibiotic administration, and postoperative nausea and vomiting prophylaxis. Challenges to the widespread use of real-time alerts and reminders include AIMS adoption rates and the difficulty in choosing appropriate areas and approaches for information systems support.

  5. Handling Flight-Research Data In Real Time

    NASA Technical Reports Server (NTRS)

    Moore, Archie L.

    1988-01-01

    Researchers at widely separated locations able to participate in tests and analyze data immediately. Basic data-handling needs common: Communicates with vehicle, pilot, and test team; Acquires, computes, and displays data; knows exact location of research vehicle at all times. Continuing challenge for designers and operators of ground support facilities to perform tasks in real time and present integrated results to research team in real time. Paper presents several approaches to satisfaction of requirements of representative types of aircraft research programs at NASA Western Aeronautical Test Range of Ames Research Center.

  6. Real-time selective sequencing using nanopore technology

    PubMed Central

    Loose, Matthew; Malla, Sunir; Stout, Michael

    2016-01-01

    The Oxford Nanopore MinION sequences DNA by sensing changes in electrical current flow in real-time as molecules traverse nanopores. Optionally, the voltage across specific nanopores can be reversed, ejecting the DNA molecule. This enables “Read Until”, the selection of specific DNA molecules for sequencing. We use dynamic time warping to match reads to reference, selecting regions of small genomes, individual amplicons, or normalization of the amplicon set. This first demonstration of direct selection of specific DNA molecules in real-time enables many novel future applications. PMID:27454285

  7. Time will show: real time predictions during interpersonal action perception.

    PubMed

    Manera, Valeria; Schouten, Ben; Verfaillie, Karl; Becchio, Cristina

    2013-01-01

    Predictive processes are crucial not only for interpreting the actions of individual agents, but also to predict how, in the context of a social interaction between two agents, the actions of one agent relate to the actions of a second agent. In the present study we investigated whether, in the context of a communicative interaction between two agents, observers can use the actions of one agent to predict when the action of a second agent will take place. Participants observed point-light displays of two agents (A and B) performing separate actions. In the communicative condition, the action performed by agent B responded to a communicative gesture performed by agent A. In the individual condition, agent A's communicative action was substituted with a non-communicative action. For each condition, we manipulated the temporal coupling of the actions of the two agents, by varying the onset of agent A's action. Using a simultaneous masking detection task, we demonstrated that the timing manipulation had a critical effect on the communicative condition, with the visual discrimination of agent B increasing linearly while approaching the original interaction timing. No effect of the timing manipulation was found for the individual condition. Our finding complements and extends previous evidence for interpersonal predictive coding, suggesting that the communicative gestures of one agent can serve not only to predict what the second agent will do, but also when his/her action will take place.

  8. The improved broadband Real-Time Seismic Network in Romania

    NASA Astrophysics Data System (ADS)

    Neagoe, C.; Ionescu, C.

    2009-04-01

    Starting with 2002 the National Institute for Earth Physics (NIEP) has developed its real-time digital seismic network. This network consists of 96 seismic stations of which 48 broad band and short period stations and two seismic arrays are transmitted in real-time. The real time seismic stations are equipped with Quanterra Q330 and K2 digitizers, broadband seismometers (STS2, CMG40T, CMG 3ESP, CMG3T) and strong motions sensors Kinemetrics episensors (+/- 2g). SeedLink and AntelopeTM (installed on MARMOT) program packages are used for real-time (RT) data acquisition and exchange. The communication from digital seismic stations to the National Data Center in Bucharest is assured by 5 providers (GPRS, VPN, satellite communication, radio lease line and internet), which will assure the back-up communications lines. The processing centre runs BRTT's AntelopeTM 4.10 data acquisition and processing software on 2 workstations for real-time processing and post processing. The Antelope Real-Time System is also providing automatic event detection, arrival picking, event location and magnitude calculation. It provides graphical display and reporting within near-real-time after a local or regional event occurred. Also at the data center was implemented a system to collect macroseismic information using the internet on which macro seismic intensity maps are generated. In the near future at the data center will be install Seiscomp 3 data acquisition processing software on a workstation. The software will run in parallel with Antelope software as a back-up. The present network will be expanded in the near future. In the first half of 2009 NIEP will install 8 additional broad band stations in Romanian territory, which also will be transmitted to the data center in real time. The Romanian Seismic Network is permanently exchanging real -time waveform data with IRIS, ORFEUS and different European countries through internet. In Romania, magnitude and location of an earthquake are now

  9. High-fidelity real-time maritime scene rendering

    NASA Astrophysics Data System (ADS)

    Shyu, Hawjye; Taczak, Thomas M.; Cox, Kevin; Gover, Robert; Maraviglia, Carlos; Cahill, Colin

    2011-06-01

    The ability to simulate authentic engagements using real-world hardware is an increasingly important tool. For rendering maritime environments, scene generators must be capable of rendering radiometrically accurate scenes with correct temporal and spatial characteristics. When the simulation is used as input to real-world hardware or human observers, the scene generator must operate in real-time. This paper introduces a novel, real-time scene generation capability for rendering radiometrically accurate scenes of backgrounds and targets in maritime environments. The new model is an optimized and parallelized version of the US Navy CRUISE_Missiles rendering engine. It was designed to accept environmental descriptions and engagement geometry data from external sources, render a scene, transform the radiometric scene using the electro-optical response functions of a sensor under test, and output the resulting signal to real-world hardware. This paper reviews components of the scene rendering algorithm, and details the modifications required to run this code in real-time. A description of the simulation architecture and interfaces to external hardware and models is presented. Performance assessments of the frame rate and radiometric accuracy of the new code are summarized. This work was completed in FY10 under Office of Secretary of Defense (OSD) Central Test and Evaluation Investment Program (CTEIP) funding and will undergo a validation process in FY11.

  10. A multiprocessing architecture for real-time monitoring

    NASA Technical Reports Server (NTRS)

    Schmidt, James L.; Kao, Simon M.; Read, Jackson Y.; Weitzenkamp, Scott M.; Laffey, Thomas J.

    1988-01-01

    A multitasking architecture for performing real-time monitoring and analysis using knowledge-based problem solving techniques is described. To handle asynchronous inputs and perform in real time, the system consists of three or more distributed processes which run concurrently and communicate via a message passing scheme. The Data Management Process acquires, compresses, and routes the incoming sensor data to other processes. The Inference Process consists of a high performance inference engine that performs a real-time analysis on the state and health of the physical system. The I/O Process receives sensor data from the Data Management Process and status messages and recommendations from the Inference Process, updates its graphical displays in real time, and acts as the interface to the console operator. The distributed architecture has been interfaced to an actual spacecraft (NASA's Hubble Space Telescope) and is able to process the incoming telemetry in real-time (i.e., several hundred data changes per second). The system is being used in two locations for different purposes: (1) in Sunnyville, California at the Space Telescope Test Control Center it is used in the preflight testing of the vehicle; and (2) in Greenbelt, Maryland at NASA/Goddard it is being used on an experimental basis in flight operations for health and safety monitoring.

  11. A Practical Approach to Implementing Real-Time Semantics

    NASA Technical Reports Server (NTRS)

    Luettgen, Gerald; Bhat, Girish; Cleaveland, Rance

    1999-01-01

    This paper investigates implementations of process algebras which are suitable for modeling concurrent real-time systems. It suggests an approach for efficiently implementing real-time semantics using dynamic priorities. For this purpose a proces algebra with dynamic priority is defined, whose semantics corresponds one-to-one to traditional real-time semantics. The advantage of the dynamic-priority approach is that it drastically reduces the state-space sizes of the systems in question while preserving all properties of their functional and real-time behavior. The utility of the technique is demonstrated by a case study which deals with the formal modeling and verification of the SCSI-2 bus-protocol. The case study is carried out in the Concurrency Workbench of North Carolina, an automated verification tool in which the process algebra with dynamic priority is implemented. It turns out that the state space of the bus-protocol model is about an order of magnitude smaller than the one resulting from real-time semantics. The accuracy of the model is proved by applying model checking for verifying several mandatory properties of the bus protocol.

  12. Spatio-temporal modeling for real-time ozone forecasting.

    PubMed

    Paci, Lucia; Gelfand, Alan E; Holland, David M

    2013-05-01

    The accurate assessment of exposure to ambient ozone concentrations is important for informing the public and pollution monitoring agencies about ozone levels that may lead to adverse health effects. High-resolution air quality information can offer significant health benefits by leading to improved environmental decisions. A practical challenge facing the U.S. Environmental Protection Agency (USEPA) is to provide real-time forecasting of current 8-hour average ozone exposure over the entire conterminous United States. Such real-time forecasting is now provided as spatial forecast maps of current 8-hour average ozone defined as the average of the previous four hours, current hour, and predictions for the next three hours. Current 8-hour average patterns are updated hourly throughout the day on the EPA-AIRNow web site. The contribution here is to show how we can substantially improve upon current real-time forecasting systems. To enable such forecasting, we introduce a downscaler fusion model based on first differences of real-time monitoring data and numerical model output. The model has a flexible coefficient structure and uses an efficient computational strategy to fit model parameters. Our hybrid computational strategy blends continuous background updated model fitting with real-time predictions. Model validation analyses show that we are achieving very accurate and precise ozone forecasts.

  13. Real-time pair-feeding of animals

    NASA Technical Reports Server (NTRS)

    Leon, H. A.; Connolly, J. P.; Hitchman, M. J.; Humbert, J. E.

    1972-01-01

    Automatic pair-feeding system was developed which immediately dispenses same amount of food to control animal as has been consumed by experimental animal that has free access to food. System consists of: master feeding system; slave feeding station; and control mechanism. Technique performs real time pair-feeding without attendant time lag.

  14. DETECTION OF FECAL ENTEROCOCCI USING A REAL TIME PCR METHOD

    EPA Science Inventory

    In spite of their importance in public health, the detection of fecal enterococci is performed via culturing methods that are time consuming and that are subject to inaccuracies that relate to their culturable status. In order to address these problems, a real time PCR (TaqMan) ...

  15. Building influenza surveillance pyramids in near real time, Australia.

    PubMed

    Dalton, Craig B; Carlson, Sandra J; Butler, Michelle T; Elvidge, Elissa; Durrheim, David N

    2013-11-01

    A timely measure of circulating influenza virus severity has been elusive. Flutracking, the Australian online influenza-like illness surveillance system, was used to construct a surveillance pyramid in near real time for 2011/2012 participants and demonstrated a striking difference between years. Such pyramids will facilitate rapid estimation of attack rates and disease severity.

  16. Toward real-time performance benchmarks for Ada

    NASA Technical Reports Server (NTRS)

    Clapp, Russell M.; Duchesneau, Louis; Volz, Richard A.; Mudge, Trevor N.; Schultze, Timothy

    1986-01-01

    The issue of real-time performance measurements for the Ada programming language through the use of benchmarks is addressed. First, the Ada notion of time is examined and a set of basic measurement techniques are developed. Then a set of Ada language features believed to be important for real-time performance are presented and specific measurement methods discussed. In addition, other important time related features which are not explicitly part of the language but are part of the run-time related features which are not explicitly part of the language but are part of the run-time system are also identified and measurement techniques developed. The measurement techniques are applied to the language and run-time system features and the results are presented.

  17. Distributed simulation using a real-time shared memory network

    NASA Technical Reports Server (NTRS)

    Simon, Donald L.; Mattern, Duane L.; Wong, Edmond; Musgrave, Jeffrey L.

    1993-01-01

    The Advanced Control Technology Branch of the NASA Lewis Research Center performs research in the area of advanced digital controls for aeronautic and space propulsion systems. This work requires the real-time implementation of both control software and complex dynamical models of the propulsion system. We are implementing these systems in a distributed, multi-vendor computer environment. Therefore, a need exists for real-time communication and synchronization between the distributed multi-vendor computers. A shared memory network is a potential solution which offers several advantages over other real-time communication approaches. A candidate shared memory network was tested for basic performance. The shared memory network was then used to implement a distributed simulation of a ramjet engine. The accuracy and execution time of the distributed simulation was measured and compared to the performance of the non-partitioned simulation. The ease of partitioning the simulation, the minimal time required to develop for communication between the processors and the resulting execution time all indicate that the shared memory network is a real-time communication technique worthy of serious consideration.

  18. A new real-time tsunami detection algorithm

    NASA Astrophysics Data System (ADS)

    Chierici, Francesco; Embriaco, Davide; Pignagnoli, Luca

    2017-01-01

    Real-time tsunami detection algorithms play a key role in any Tsunami Early Warning System. We have developed a new algorithm for tsunami detection based on the real-time tide removal and real-time band-pass filtering of seabed pressure recordings. The algorithm greatly increases the tsunami detection probability, shortens the detection delay and enhances detection reliability with respect to the most widely used tsunami detection algorithm, while containing the computational cost. The algorithm is designed to be used also in autonomous early warning systems with a set of input parameters and procedures which can be reconfigured in real time. We have also developed a methodology based on Monte Carlo simulations to test the tsunami detection algorithms. The algorithm performance is estimated by defining and evaluating statistical parameters, namely the detection probability, the detection delay, which are functions of the tsunami amplitude and wavelength, and the occurring rate of false alarms. Pressure data sets acquired by Bottom Pressure Recorders in different locations and environmental conditions have been used in order to consider real working scenarios in the test. We also present an application of the algorithm to the tsunami event which occurred at Haida Gwaii on 28 October 2012 using data recorded by the Bullseye underwater node of Ocean Networks Canada. The algorithm successfully ran for test purpose in year-long missions onboard abyssal observatories, deployed in the Gulf of Cadiz and in the Western Ionian Sea.

  19. Test applications for heterogeneous real-time network testbed

    SciTech Connect

    Mines, R.F.; Knightly, E.W.

    1994-07-01

    This paper investigates several applications for a heterogeneous real-time network testbed. The network is heterogeneous in terms of network devices, technologies, protocols, and algorithms. The network is real-time in that its services can provide per-connection end-to-end performance guarantees. Although different parts of the network use different algorithms, all components have the necessary mechanisms to provide performance guarantees: admission control and priority scheduling. Three applications for this network are described in this paper: a video conferencing tool, a tool for combustion modeling using distributed computing, and an MPEG video archival system. Each has minimum performance requirements that must be provided by the network. By analyzing these applications, we provide insights to the traffic characteristics and performance requirements of practical real-time loads.

  20. Seismic monitoring and real time damage estimation for lifelines

    SciTech Connect

    Takada, Shiro; Ogawa, Yasuo

    1995-12-31

    This paper presents a methodology for estimating the number of damaged locations of lifeline systems based on seismic monitoring ground motions taking consideration of liquefaction phenomena. The liquefaction area and the number of damage to the lifeline facilities can be synthetically indicated by a personal computer program developed for this purpose. The method is based on the real time observed data of strong ground motions. Osaka Gas Company had been developing the real time damage simulation system by planning to set additional tele-meter seismographs when the Hanshin Great Earthquake occurred. Other lifelines such as electricity, telecommunication, water and sewage systems in quake-hit area had not such a real time damage simulation tool based on observed ground motions. On the other hand, these lifelines have their own damage detection system such as pressure, flow, relay signal, etc.

  1. Real-Time Statistical Modeling of Blood Sugar.

    PubMed

    Otoom, Mwaffaq; Alshraideh, Hussam; Almasaeid, Hisham M; López-de-Ipiña, Diego; Bravo, José

    2015-10-01

    Diabetes is considered a chronic disease that incurs various types of cost to the world. One major challenge in the control of Diabetes is the real time determination of the proper insulin dose. In this paper, we develop a prototype for real time blood sugar control, integrated with the cloud. Our system controls blood sugar by observing the blood sugar level and accordingly determining the appropriate insulin dose based on patient's historical data, all in real time and automatically. To determine the appropriate insulin dose, we propose two statistical models for modeling blood sugar profiles, namely ARIMA and Markov-based model. Our experiment used to evaluate the performance of the two models shows that the ARIMA model outperforms the Markov-based model in terms of prediction accuracy.

  2. Real time capable infrared thermography for ASDEX Upgrade

    NASA Astrophysics Data System (ADS)

    Sieglin, B.; Faitsch, M.; Herrmann, A.; Brucker, B.; Eich, T.; Kammerloher, L.; Martinov, S.

    2015-11-01

    Infrared (IR) thermography is widely used in fusion research to study power exhaust and incident heat load onto the plasma facing components. Due to the short pulse duration of today's fusion experiments, IR systems have mostly been designed for off-line data analysis. For future long pulse devices (e.g., Wendelstein 7-X, ITER), a real time evaluation of the target temperature and heat flux is mandatory. This paper shows the development of a real time capable IR system for ASDEX Upgrade. A compact IR camera has been designed incorporating the necessary magnetic and electric shielding for the detector, cooler assembly. The camera communication is based on the Camera Link industry standard. The data acquisition hardware is based on National Instruments hardware, consisting of a PXIe chassis inside and a fibre optical connected industry computer outside the torus hall. Image processing and data evaluation are performed using real time LabVIEW.

  3. The VLBA Correlator---Real-Time in the Distributed ERA

    NASA Astrophysics Data System (ADS)

    Wells, Donald C.

    1993-01-01

    The Correlator is the signal processing engine of the Very Long Baseline Array [VLBA]. Radio signals are recorded on special wideband digital recorders at the 10 VLBA antennas and are shipped to the Array Operations Center in Socorro, New Mexico, where they are played back simultaneously into the Correlator. Real-time software and firmware controls the playback drives to achieve synchronization, compute models of the wavefront delay, control the numerous modules of the Correlator, and record FITS files of the fringe visibilities at the back-end of the Correlator. The Correlator system contains a total of more than 100 programmable computers, which communicate by means of various protocols. The VLBA Correlator's dependence on network protocols is an example of the radical transformation of the real-time world over the past five years: real-time is becoming more like conventional computing.

  4. Real-time structured light intraoral 3D measurement pipeline

    NASA Astrophysics Data System (ADS)

    Gheorghe, Radu; Tchouprakov, Andrei; Sokolov, Roman

    2013-02-01

    Computer aided design and manufacturing (CAD/CAM) is increasingly becoming a standard feature and service provided to patients in dentist offices and denture manufacturing laboratories. Although the quality of the tools and data has slowly improved in the last years, due to various surface measurement challenges, practical, accurate, invivo, real-time 3D high quality data acquisition and processing still needs improving. Advances in GPU computational power have allowed for achieving near real-time 3D intraoral in-vivo scanning of patient's teeth. We explore in this paper, from a real-time perspective, a hardware-software-GPU solution that addresses all the requirements mentioned before. Moreover we exemplify and quantify the hard and soft deadlines required by such a system and illustrate how they are supported in our implementation.

  5. Kalman filtering for real-time navigator processing.

    PubMed

    Spincemaille, Pascal; Nguyen, Thanh D; Prince, Martin R; Wang, Yi

    2008-07-01

    Navigator echoes are used in high-resolution cardiac MRI for tracking physiological motion to suppress motion artifacts. Alternatives to the conventional diaphragm navigator such as the cardiac fat navigator and the k-space center signal (self-navigator) were developed to monitor heart motion directly. These navigator data can be noisy or may contain undesirable frequency components. Real-time filtering of navigator data without delay, as opposed to the previously used retrospective frequency band filtering, is required for effective prospective navigator gating. One of the commonly used real-time filtering techniques is the Kalman filter, which adaptively estimates motion and suppresses measurement noise by using Bayesian statistics and a motion model. The Kalman filter is investigated in this work to filter noise and distinguish cardiac and respiratory components in navigator data. Preliminary imaging data demonstrate the feasibility of real-time Kalman filtering for prospective respiratory self-gating in CINE cardiac MRI.

  6. Towards real-time registration of 4D ultrasound images.

    PubMed

    Foroughi, Pezhman; Abolmaesumi, Purang; Hashtrudi-Zaad, Keyvan

    2006-01-01

    In this paper, we demonstrate a method for fast registration of sequences of 3D liver images, which could be used for the future real-time applications. In our method, every image is elastically registered to a so called fixed ultrasound image exploiting the information from previous registration. A few feature points are automatically selected, and tracked inside the images, while the deformation of other points are extrapolated with respect to the tracked points employing a fast free-form approach. The main intended application of the proposed method is real-time tracking of tumors for radiosurgery. The algorithm is evaluated on both naturally and artificially deformed images. Experimental results show that for around 85 percent accuracy, the process of tracking is completed very close to real time.

  7. Real-time measurement of mental workload: A feasibility study

    NASA Technical Reports Server (NTRS)

    Kramer, Arthur; Humphrey, Darryl; Sirevaag, Erik; Mecklinger, Axel

    1990-01-01

    The primary goal of the study was to explore the utility of event-related brain potentials (ERP) as real-time measures of workload. To this end, subjects performed two different tasks both separately and together. One task required that subjects monitor a bank of constantly changing gauges and detect critical deviations. Difficulty was varied by changing the predictability of the gauges. The second task was mental arithmetic. Difficulty was varied by requiring subjects to perform operations on either two or three columns of numbers. Two conditions that could easily be distinguished on the basis of performance measures were selected for the real-time evaluation of ERPs. A bootstrapping approach was adopted in which one thousand samples of n trials (n = 1, 3, 5 ...65) were classified using several measures of P300 and Slow Wave amplitude. Classification accuracies of 85 percent were achieved with 25 trials. Results are discussed in terms of potential enhancements for real-time recording.

  8. Real-time RNA profiling within a single bacterium.

    PubMed

    Le, Thuc T; Harlepp, Sébastien; Guet, Calin C; Dittmar, Kimberly; Emonet, Thierry; Pan, Tao; Cluzel, Philippe

    2005-06-28

    Characterizing the dynamics of specific RNA levels requires real-time RNA profiling in a single cell. We show that the combination of a synthetic modular genetic system with fluorescence correlation spectroscopy allows us to directly measure in real time the activity of any specific promoter in prokaryotes. Using a simple inducible gene expression system, we found that induced RNA levels within a single bacterium of Escherichia coli exhibited a pulsating profile in response to a steady input of inducer. The genetic deletion of an efflux pump system, a key determinant of antibiotic resistance, altered the pulsating transcriptional dynamics and caused overexpression of induced RNA. In contrast with population measurements, real-time RNA profiling permits identifying relationships between genotypes and transcriptional dynamics that are accessible only at the level of the single cell.

  9. Towards real-time medical diagnostics using hyperspectral imaging technology

    NASA Astrophysics Data System (ADS)

    Bjorgan, Asgeir; Randeberg, Lise L.

    2015-07-01

    Hyperspectral imaging provides non-contact, high resolution spectral images which has a substantial diagnostic potential. This can be used for e.g. diagnosis and early detection of arthritis in finger joints. Processing speed is currently a limitation for clinical use of the technique. A real-time system for analysis and visualization using GPU processing and threaded CPU processing is presented. Images showing blood oxygenation, blood volume fraction and vessel enhanced images are among the data calculated in real-time. This study shows the potential of real-time processing in this context. A combination of the processing modules will be used in detection of arthritic finger joints from hyperspectral reflectance and transmittance data.

  10. Real-time experiment interface for biological control applications.

    PubMed

    Lin, Risa J; Bettencourt, Jonathan; Wha Ite, John; Christini, David J; Butera, Robert J

    2010-01-01

    The Real-time Experiment Interface (RTXI) is a fast and versatile real-time biological experimentation system based on Real-Time Linux. RTXI is open source and free, can be used with an extensive range of experimentation hardware, and can be run on Linux or Windows computers (when using the Live CD). RTXI is currently used extensively for two experiment types: dynamic patch clamp and closed-loop stimulation pattern control in neural and cardiac single cell electrophysiology. RTXI includes standard plug-ins for implementing commonly used electrophysiology protocols with synchronized stimulation, event detection, and online analysis. These and other user-contributed plug-ins can be found on the website (http://www.rtxi.org).

  11. Continuous real-time water information: an important Kansas resource

    USGS Publications Warehouse

    Loving, Brian L.; Putnam, James E.; Turk, Donita M.

    2014-01-01

    Continuous real-time information on streams, lakes, and groundwater is an important Kansas resource that can safeguard lives and property, and ensure adequate water resources for a healthy State economy. The U.S. Geological Survey (USGS) operates approximately 230 water-monitoring stations at Kansas streams, lakes, and groundwater sites. Most of these stations are funded cooperatively in partnerships with local, tribal, State, or other Federal agencies. The USGS real-time water-monitoring network provides long-term, accurate, and objective information that meets the needs of many customers. Whether the customer is a water-management or water-quality agency, an emergency planner, a power or navigational official, a farmer, a canoeist, or a fisherman, all can benefit from the continuous real-time water information gathered by the USGS.

  12. Real-Time Parameter Estimation in the Frequency Domain

    NASA Technical Reports Server (NTRS)

    Morelli, Eugene A.

    2000-01-01

    A method for real-time estimation of parameters in a linear dynamic state-space model was developed and studied. The application is aircraft dynamic model parameter estimation from measured data in flight. Equation error in the frequency domain was used with a recursive Fourier transform for the real-time data analysis. Linear and nonlinear simulation examples and flight test data from the F-18 High Alpha Research Vehicle were used to demonstrate that the technique produces accurate model parameter estimates with appropriate error bounds. Parameter estimates converged in less than one cycle of the dominant dynamic mode, using no a priori information, with control surface inputs measured in flight during ordinary piloted maneuvers. The real-time parameter estimation method has low computational requirements and could be implemented

  13. Real time capable infrared thermography for ASDEX Upgrade

    SciTech Connect

    Sieglin, B. Faitsch, M.; Herrmann, A.; Brucker, B.; Eich, T.; Kammerloher, L.; Martinov, S.

    2015-11-15

    Infrared (IR) thermography is widely used in fusion research to study power exhaust and incident heat load onto the plasma facing components. Due to the short pulse duration of today’s fusion experiments, IR systems have mostly been designed for off-line data analysis. For future long pulse devices (e.g., Wendelstein 7-X, ITER), a real time evaluation of the target temperature and heat flux is mandatory. This paper shows the development of a real time capable IR system for ASDEX Upgrade. A compact IR camera has been designed incorporating the necessary magnetic and electric shielding for the detector, cooler assembly. The camera communication is based on the Camera Link industry standard. The data acquisition hardware is based on National Instruments hardware, consisting of a PXIe chassis inside and a fibre optical connected industry computer outside the torus hall. Image processing and data evaluation are performed using real time LabVIEW.

  14. New real-time strain imaging concepts using diagnostic ultrasound.

    PubMed

    Pesavento, A; Lorenz, A; Siebers, S; Ermert, H

    2000-06-01

    Two real-time strain imaging concepts and systems are presented. Both systems are based on a conventional ultrasound scanner that is connected to a PC with an A/D converter card for real-time data acquisition of rf data. Differential strain between successively acquired rf frames are estimated using phase root seeking. The first concept uses a special real-time implementation of manual elastography. In the second concept, denoted 'vibrography', the static compression is replaced by low-frequency axial vibration of the probe, still operating in quasistatic acquisition mode. The properties of both concepts are discussed with regard to noise and motion artefacts, and it is shown, using simulations and phantom experiments, that both imaging concepts yield the same kind of strain images. Vibrography has the advantage that no manual compression has to be applied, total compression can be very low and some motion artefacts are better suppressed.

  15. New real-time strain imaging concepts using diagnostic ultrasound

    NASA Astrophysics Data System (ADS)

    Pesavento, A.; Lorenz, A.; Siebers, S.; Ermert, H.

    2000-06-01

    Two real-time strain imaging concepts and systems are presented. Both systems are based on a conventional ultrasound scanner that is connected to a PC with an A/D converter card for real-time data acquisition of rf data. Differential strain between successively acquired rf frames are estimated using phase root seeking. The first concept uses a special real-time implementation of manual elastography. In the second concept, denoted `vibrography', the static compression is replaced by low-frequency axial vibration of the probe, still operating in quasistatic acquisition mode. The properties of both concepts are discussed with regard to noise and motion artefacts, and it is shown, using simulations and phantom experiments, that both imaging concepts yield the same kind of strain images. Vibrography has the advantage that no manual compression has to be applied, total compression can be very low and some motion artefacts are better suppressed.

  16. Assay of gliadin by real-time immunopolymerase chain reaction.

    PubMed

    Henterich, Nadine; Osman, Awad A; Méndez, Enrique; Mothes, Thomas

    2003-10-01

    Patients with coeliac disease (gluten-sensitive enteropathy) are intolerant against gliadins from wheat and the respective proteins from related cereals and have to keep a lifelong gluten-free diet. For control of gliadin in gluten-free food sensitive assay techniques are necessary. We developed an immunopolymerase chain reaction (iPCR) assay for gliadin. In this technique immunological detection of gliadin by a monoclonal antibody R5 conjugated with an oligonucleotide is amplified by PCR. For quantification, iPCR was performed as real-time PCR (real-time iPCR) in one step. By means of real-time iPCR, the sensitivity of gliadin analysis was increased more than 30-fold above the level reached by enzyme immunoassay. Real time-iPCR using R5 directly conjugated with oligonucleotide was clearly more sensitive than real time-iPCR applying sequentially biotinylated R5, streptavidin, and biotinylated oligonucleotide. With directly conjugated R5 gliadin was detected at a concentration as low as 0.16 ng/mL corresponding to 16 microg gliadin/100 g food or 0.16 ppm (corresponding to 0.25 g of food extracted in 10 mL of solvent and 25-fold dilution of the extract prior to analysis). This is the first report applying the highly sensitive technique of iPCR for gliadin analysis. Furthermore, this is the first approach to perform real-time iPCR in one step without changing the reaction vessels after enzyme immunoassay for subsequent PCR analysis thus minimizing risks of contamination and loss of sensitivity.

  17. Simultaneous real-time monitoring of multiple cortical systems

    NASA Astrophysics Data System (ADS)

    Gupta, Disha; Hill, N. Jeremy; Brunner, Peter; Gunduz, Aysegul; Ritaccio, Anthony L.; Schalk, Gerwin

    2014-10-01

    Objective. Real-time monitoring of the brain is potentially valuable for performance monitoring, communication, training or rehabilitation. In natural situations, the brain performs a complex mix of various sensory, motor or cognitive functions. Thus, real-time brain monitoring would be most valuable if (a) it could decode information from multiple brain systems simultaneously, and (b) this decoding of each brain system were robust to variations in the activity of other (unrelated) brain systems. Previous studies showed that it is possible to decode some information from different brain systems in retrospect and/or in isolation. In our study, we set out to determine whether it is possible to simultaneously decode important information about a user from different brain systems in real time, and to evaluate the impact of concurrent activity in different brain systems on decoding performance. Approach. We study these questions using electrocorticographic signals recorded in humans. We first document procedures for generating stable decoding models given little training data, and then report their use for offline and for real-time decoding from 12 subjects (six for offline parameter optimization, six for online experimentation). The subjects engage in tasks that involve movement intention, movement execution and auditory functions, separately, and then simultaneously. Main results. Our real-time results demonstrate that our system can identify intention and movement periods in single trials with an accuracy of 80.4% and 86.8%, respectively (where 50% would be expected by chance). Simultaneously, the decoding of the power envelope of an auditory stimulus resulted in an average correlation coefficient of 0.37 between the actual and decoded power envelopes. These decoders were trained separately and executed simultaneously in real time. Significance. This study yielded the first demonstration that it is possible to decode simultaneously the functional activity of multiple

  18. An approach to a real-time distribution system

    NASA Technical Reports Server (NTRS)

    Kittle, Frank P., Jr.; Paddock, Eddie J.; Pocklington, Tony; Wang, Lui

    1990-01-01

    The requirements of a real-time data distribution system are to provide fast, reliable delivery of data from source to destination with little or no impact to the data source. In this particular case, the data sources are inside an operational environment, the Mission Control Center (MCC), and any workstation receiving data directly from the operational computer must conform to the software standards of the MCC. In order to supply data to development workstations outside of the MCC, it is necessary to use gateway computers that prevent unauthorized data transfer back to the operational computers. Many software programs produced on the development workstations are targeted for real-time operation. Therefore, these programs must migrate from the development workstation to the operational workstation. It is yet another requirement for the Data Distribution System to ensure smooth transition of the data interfaces for the application developers. A standard data interface model has already been set up for the operational environment, so the interface between the distribution system and the application software was developed to match that model as closely as possible. The system as a whole therefore allows the rapid development of real-time applications without impacting the data sources. In summary, this approach to a real-time data distribution system provides development users outside of the MCC with an interface to MCC real-time data sources. In addition, the data interface was developed with a flexible and portable software design. This design allows for the smooth transition of new real-time applications to the MCC operational environment.

  19. Terahertz real-time imaging for nondestructive detection

    NASA Astrophysics Data System (ADS)

    Zhang, LiangLiang; Karpowicz, Nick; Zhang, CunLin; Zhao, YueJin; Zhang, XiCheng

    2008-03-01

    We present a real time imaging measurement in the terahertz (THz) frequency region. The dynamic subtraction technique is used to reduce long-term optical background drift. The reflective images of two targets, a Nikon camera's lens cap and a plastic toy gun, are obtained. For the lens cap, the image data were processed to be false color images. For the toy gun, we show that even under an optically opaque canvas bag, a clear terahertz image is obtained. It is shown that terahertz real time imaging can be used to nondestructively detect concealed objects.

  20. Real-time nondestructive imaging with THz waves

    NASA Astrophysics Data System (ADS)

    Zhang, LiangLiang; Karpowicz, Nick; Zhang, CunLin; Zhao, YueJin; Zhang, XiCheng

    2008-03-01

    We present a real-time imaging measurement in the terahertz (THz) frequency region. The dynamic subtraction technique is used to reduce long-term optical background drift. The reflective images of two targets, a Nikon camera's lens cap and a plastic toy gun, are obtained. For the lens cap, the image data were processed to be false-color images. For the toy gun, we show that even under an optically opaque canvas bag, a clear terahertz image is obtained. It is shown that terahertz real-time imaging can be used to nondestructively detect concealed objects.