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Sample records for requirements specification srs

  1. Surveillance Analysis Computer System (SACS) software requirements specification (SRS)

    SciTech Connect

    Glasscock, J.A.; Flanagan, M.J.

    1995-09-01

    This document is the primary document establishing requirements for the Surveillance Analysis Computer System (SACS) Database, an Impact Level 3Q system. The purpose is to provide the customer and the performing organization with the requirements for the SACS Project.

  2. Surveillance Analysis Computer System (SACS): Software requirements specification (SRS). Revision 2

    SciTech Connect

    Glasscock, J.A.

    1995-03-08

    This document is the primary document establishing requirements for the Surveillance Analysis Computer System (SACS) database, an Impact Level 3Q system. SACS stores information on tank temperatures, surface levels, and interstitial liquid levels. This information is retrieved by the customer through a PC-based interface and is then available to a number of other software tools. The software requirements specification (SRS) describes the system requirements for the SACS Project, and follows the Standard Engineering Practices (WHC-CM-6-1), Software Practices (WHC-CM-3-10) and Quality Assurance (WHC-CM-4-2, QR 19.0) policies.

  3. Recognition of SUMO-modified PCNA requires tandem receptor motifs in Srs2

    SciTech Connect

    Armstrong, Anthony A.; Mohideen, Firaz; Lima, Christopher D.

    2013-04-08

    Ubiquitin (Ub) and ubiquitin-like (Ubl) modifiers such as SUMO (also known as Smt3 in Saccharomyces cerevisiae) mediate signal transduction through post-translational modification of substrate proteins in pathways that control differentiation, apoptosis and the cell cycle, and responses to stress such as the DNA damage response. In yeast, the proliferating cell nuclear antigen PCNA (also known as Pol30) is modified by ubiquitin in response to DNA damage and by SUMO during S phase. Whereas Ub-PCNA can signal for recruitment of translesion DNA polymerases, SUMO-PCNA signals for recruitment of the anti-recombinogenic DNA helicase Srs2. It remains unclear how receptors such as Srs2 specifically recognize substrates after conjugation to Ub and Ubls. Here we show, through structural, biochemical and functional studies, that the Srs2 carboxy-terminal domain harbors tandem receptor motifs that interact independently with PCNA and SUMO and that both motifs are required to recognize SUMO-PCNA specifically. The mechanism presented is pertinent to understanding how other receptors specifically recognize Ub- and Ubl-modified substrates to facilitate signal transduction.

  4. Business System Planning Project System Requirements Specification

    SciTech Connect

    NELSON, R.E.

    2000-09-08

    The purpose of the Business Systems Planning Project System Requirements Specification (SRS) is to provide the outline and contents of the requirements for the CH2M HILL Hanford Group, Inc. (CHG) integrated business and technical information systems. The SRS will translate proposed objectives into the statement of the functions that are to be performed and data and information flows that they require. The requirements gathering methodology will use (1) facilitated group requirement sessions; (2) individual interviews; (3) surveys; and (4) document reviews. The requirements will be verified and validated through coordination of the technical requirement team and CHG Managers. The SRS document used the content and format specified in Lockheed Martin Services, Inc. Organization Standard Software Practices in conjunction with the Institute of Electrical and Electronics Engineers Standard 8340-1984 for Systems Requirements Documents.

  5. Specifics of processing SRS lidar signals in GHz frequency range

    NASA Astrophysics Data System (ADS)

    Grishkanich, A. S.; Elizarov, V. V.; Kascheev, S. V.; Zhevlakov, A. P.; Sidorov, I. S.

    2015-12-01

    One of the highly effective methods of operative remote environmental monitoring on land and water surfaces is laser sensing. It knew that the Raman scattering cross section is very small (10-25-10-27), so in some cases radiation back into captivity to the target could be a few tens of photons. For high-speed sensing, speed of processing and ease of use lidar units required for the use of appropriate hardware and software systems used for the decision of tasks of collecting, processing, storing, organizing large amounts of data.

  6. Pro-recombination Role of Srs2 Protein Requires SUMO (Small Ubiquitin-like Modifier) but Is Independent of PCNA (Proliferating Cell Nuclear Antigen) Interaction.

    PubMed

    Kolesar, Peter; Altmannova, Veronika; Silva, Sonia; Lisby, Michael; Krejci, Lumir

    2016-04-01

    Srs2 plays many roles in DNA repair, the proper regulation and coordination of which is essential. Post-translational modification by small ubiquitin-like modifier (SUMO) is one such possible mechanism. Here, we investigate the role of SUMO in Srs2 regulation and show that the SUMO-interacting motif (SIM) of Srs2 is important for the interaction with several recombination factors. Lack of SIM, but not proliferating cell nuclear antigen (PCNA)-interacting motif (PIM), leads to increased cell death under circumstances requiring homologous recombination for DNA repair. Simultaneous mutation of SIM in asrs2ΔPIMstrain leads to a decrease in recombination, indicating a pro-recombination role of SUMO. Thus SIM has an ambivalent function in Srs2 regulation; it not only mediates interaction with SUMO-PCNA to promote the anti-recombination function but it also plays a PCNA-independent pro-recombination role, probably by stimulating the formation of recombination complexes. The fact that deletion of PIM suppresses the phenotypes of Srs2 lacking SIM suggests that proper balance between the anti-recombination PCNA-bound and pro-recombination pools of Srs2 is crucial. Notably, sumoylation of Srs2 itself specifically stimulates recombination at the rDNA locus.

  7. Investigating the clinical usefulness of the Social Responsiveness Scale (SRS) in a tertiary level, autism spectrum disorder specific assessment clinic.

    PubMed

    Aldridge, Fiona J; Gibbs, Vicki M; Schmidhofer, Katherine; Williams, Megan

    2012-02-01

    The Social Responsiveness Scale (SRS; Constantino and Gruber in Social Responsiveness Scale (SRS). Western Psychological Services, Los Angeles, 2005) is a commonly used screening tool for identifying children with possible autism spectrum disorder (ASD). This study investigated the relationship between SRS scores and eventual diagnostic outcome for children referred to a tertiary level, autism specific assessment service. Forty eight children (mean age = 8.10; 92% male) underwent a comprehensive ASD assessment. Parent and teacher SRS scores were subsequently compared with diagnostic outcome. Sensitivity was high (91% for parent report; 84% for teacher report), however specificity was much lower (8% for parent report; 41% for teacher report). Results demonstrate a need for caution when interpreting SRS results based on current cut-off scores, particularly in children with previously identified social developmental problems. PMID:21516433

  8. Highly specific label-free molecular imaging with spectrally tailored excitation-stimulated Raman scattering (STE-SRS) microscopy

    NASA Astrophysics Data System (ADS)

    Freudiger, Christian W.; Min, Wei; Holtom, Gary R.; Xu, Bingwei; Dantus, Marcos; Sunney Xie, X.

    2011-02-01

    Label-free microscopy that has chemical contrast and high acquisition speeds up to video rates has recently been made possible using stimulated Raman scattering (SRS) microscopy. SRS imaging offers high sensitivity, but the spectral specificity of the original narrowband implementation is limited, making it difficult to distinguish chemical species with overlapping Raman bands. Here, we present a highly specific imaging method that allows mapping of a particular chemical species in the presence of interfering species, based on tailored multiplex excitation of its vibrational spectrum. This is implemented by spectral modulation of a broadband pump beam at a high frequency (>1 MHz), allowing detection of the SRS signal of the narrowband Stokes beam with high sensitivity. Using the scheme, we demonstrate quantification of cholesterol in the presence of lipids, and real-time three-dimensional spectral imaging of protein, stearic acid and oleic acid in live Caenorhabditis elegans.

  9. Software Requirements Specification Verifiable Fuel Cycle Simulation (VISION) Model

    SciTech Connect

    D. E. Shropshire; W. H. West

    2005-11-01

    The purpose of this Software Requirements Specification (SRS) is to define the top-level requirements for a Verifiable Fuel Cycle Simulation Model (VISION) of the Advanced Fuel Cycle (AFC). This simulation model is intended to serve a broad systems analysis and study tool applicable to work conducted as part of the AFCI (including costs estimates) and Generation IV reactor development studies.

  10. Why SRS Matters - Introduction

    SciTech Connect

    Hunt, Paul

    2015-01-21

    A video series presenting an overview of the Savannah River Site's (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode provides an introduction to the SRS mission and operations.

  11. Solid Waste Information and Tracking System (SWITS) Software Requirements Specification

    SciTech Connect

    MAY, D.L.

    2000-03-22

    This document is the primary document establishing requirements for the Solid Waste Information and Tracking System (SWITS) as it is converted to a client-server architecture. The purpose is to provide the customer and the performing organizations with the requirements for the SWITS in the new environment. This Software Requirement Specification (SRS) describes the system requirements for the SWITS Project, and follows the PHMC Engineering Requirements, HNF-PRO-1819, and Computer Software Qualify Assurance Requirements, HNF-PRO-309, policies. This SRS includes sections on general description, specific requirements, references, appendices, and index. The SWITS system defined in this document stores information about the solid waste inventory on the Hanford site. Waste is tracked as it is generated, analyzed, shipped, stored, and treated. In addition to inventory reports a number of reports for regulatory agencies are produced.

  12. Investigating the Clinical Usefulness of the Social Responsiveness Scale (SRS) in a Tertiary Level, Autism Spectrum Disorder Specific Assessment Clinic

    ERIC Educational Resources Information Center

    Aldridge, Fiona J.; Gibbs, Vicki M.; Schmidhofer, Katherine; Williams, Megan

    2012-01-01

    The Social Responsiveness Scale (SRS; Constantino and Gruber in Social Responsiveness Scale (SRS). Western Psychological Services, Los Angeles, 2005) is a commonly used screening tool for identifying children with possible autism spectrum disorder (ASD). This study investigated the relationship between SRS scores and eventual diagnostic outcome…

  13. The influence of Savannah River discharge and changing SRS cooling water requirements on the potential entrainment of ichthyoplankton at the SRS Savannah River intakes

    SciTech Connect

    Paller, M.H.

    1992-08-01

    Entrainment (i.e., withdrawal of fish larvae and eggs in cooling water) at the SRS Savannah River intakes is greatest when periods of high river water usage coincide with low river dischargeduring the spawning season. American shad and striped bass are the two species of greatest concern because of their recreational and/or commercial importance and because they produce drifting eggs and larvae vulnerable to entrainment. In the mid-reaches of the Savannah River, American shad and striped bass spawn primarily during April and May. An analysis of Savannah River discharge during April and May 1973--1989 indicated the potential for entrainment of 4--18% of the American shad and striped bass larvae and eggs that drifted past the SRS. This analysis assumed the concurrent operation of L-, K-, and P-Reactors. Additional scenarios investigated were: (1) shutting down L- and P-Reactors, and operating K-Reactor with a recycle cooling tower; and (2) shutting down L- and P-Reactors, eliminating minimum flows to Steel Creek, and operating K-Reactor with a recycle cooling tower. The former scenario reduced potential entrainment to 0.7--3.3%, and the latter scenario reduced potential entrainment to 0.20.8%. Thus, the currently favored scenario of operating K-Reactor with a cooling tower and not operating L- and P-Reactors represents a significant lessening of the impact of SRS operations.

  14. Master Software Requirements Specification

    NASA Technical Reports Server (NTRS)

    Hu, Chaumin

    2003-01-01

    A basic function of a computational grid such as the NASA Information Power Grid (IPG) is to allow users to execute applications on remote computer systems. The Globus Resource Allocation Manager (GRAM) provides this functionality in the IPG and many other grids at this time. While the functionality provided by GRAM clients is adequate, GRAM does not support useful features such as staging several sets of files, running more than one executable in a single job submission, and maintaining historical information about execution operations. This specification is intended to provide the environmental and software functional requirements for the IPG Job Manager V2.0 being developed by AMTI for NASA.

  15. RMACS software requirements specification

    SciTech Connect

    Gneiting, B.C.

    1996-10-01

    This document defines the essential user (or functional) requirements of the Requirements Management and Assured Compliance System (RMACS), which is used by the Tank Waste Remediation System program (TWRS). RMACS provides a computer-based environment that TWRS management and systems engineers can use to identify, define, and document requirements. The intent of the system is to manage information supporting definition of the TWRS technical baseline using a structured systems engineering process. RMACS has the capability to effectively manage a complete set of complex requirements and relationships in a manner that satisfactorily assures compliance to the program requirements over the TWRS life-cycle.

  16. High-Level software requirements specification for the TWRS controlled baseline database system

    SciTech Connect

    Spencer, S.G.

    1998-09-23

    This Software Requirements Specification (SRS) is an as-built document that presents the Tank Waste Remediation System (TWRS) Controlled Baseline Database (TCBD) in its current state. It was originally known as the Performance Measurement Control System (PMCS). Conversion to the new system name has not occurred within the current production system. Therefore, for simplicity, all references to TCBD are equivalent to PMCS references. This SRS will reference the PMCS designator from this point forward to capture the as-built SRS. This SRS is written at a high-level and is intended to provide the design basis for the PMCS. The PMCS was first released as the electronic data repository for cost, schedule, and technical administrative baseline information for the TAAS Program. During its initial development, the PMCS was accepted by the customer, TARS Business Management, with no formal documentation to capture the initial requirements.

  17. Requirements management system browser software requirements specification

    SciTech Connect

    Frank, D.D.

    1996-10-01

    The purpose of this document is to define the essential user requirements for the Requirements Management System Browser (RMSB) application. This includes specifications for the Graphical User Interface (GUI) and the supporting database structures. The RMSB application is needed to provide an easy to use PC-based interface to browse system engineering data stored and managed in a UNIX software application. The system engineering data include functions, requirements, and architectures that make up the Tank Waste Remediation System (TWRS) technical baseline. This document also covers the requirements for a software application titled ``RMSB Data Loader (RMSB- DL)``, referred to as the ``Parser.`` The Parser is needed to read and parse a data file and load the data structure supporting the Browser.

  18. Software requirements specification for the GIS-T/ISTEA pooled fund study phase C linear referencing engine

    SciTech Connect

    Amai, W.; Espinoza, J. Jr.; Fletcher, D.R.

    1997-06-01

    This Software Requirements Specification (SRS) describes the features to be provided by the software for the GIS-T/ISTEA Pooled Fund Study Phase C Linear Referencing Engine project. This document conforms to the recommendations of IEEE Standard 830-1984, IEEE Guide to Software Requirements Specification (Institute of Electrical and Electronics Engineers, Inc., 1984). The software specified in this SRS is a proof-of-concept implementation of the Linear Referencing Engine as described in the GIS-T/ISTEA pooled Fund Study Phase B Summary, specifically Sheet 13 of the Phase B object model. The software allows an operator to convert between two linear referencing methods and a datum network.

  19. Flight Guidance System Requirements Specification

    NASA Technical Reports Server (NTRS)

    Miller, Steven P.; Tribble, Alan C.; Carlson, Timothy M.; Danielson, Eric J.

    2003-01-01

    This report describes a requirements specification written in the RSML-e language for the mode logic of a Flight Guidance System of a typical regional jet aircraft. This model was created as one of the first steps in a five-year project sponsored by the NASA Langley Research Center, Rockwell Collins Inc., and the Critical Systems Research Group of the University of Minnesota to develop new methods and tools to improve the safety of avionics designs. This model will be used to demonstrate the application of a variety of methods and techniques, including safety analysis of system and subsystem requirements, verification of key properties using theorem provers and model checkers, identification of potential sources mode confusion in system designs, partitioning of applications based on the criticality of system hazards, and autogeneration of avionics quality code. While this model is representative of the mode logic of a typical regional jet aircraft, it does not describe an actual or planned product. Several aspects of a full Flight Guidance System, such as recovery from failed sensors, have been omitted, and no claims are made regarding the accuracy or completeness of this specification.

  20. Cockpit display requirements and specifications

    NASA Astrophysics Data System (ADS)

    Hopper, Darrel G.

    1993-12-01

    Flight instrument design has begun to include a new electronic technology for the display head: active matrix liquid crystal display (AMLCD). This is a significant design transition and applies across the board to complete cockpit modernization programs, individual instrument replacement projects, and new systems. AMLCD-based instruments are expected to have a substantially higher mean time between failure compared to both electromechanical and CRT- based instruments. Thus, the new technology will pay for itself. Furthermore, AMLCDs are truly sunlight-readable whereas CRT displays are not; it is mission critical that a pilot be able to see an instrument with the sun shining directly in the eye or onto the display. AMLCDs can also provide larger display areas enabling formats which increase situational awareness. As this is a new technology for the military, an industrial base for militarized AMLCDs must be created based on present research capabilities. The requirements for AMLCDs in DOD programs have been analyzed. Projects to build infrastructure and capacity are described. Applications include not only cockpits, but also digital map/GPS integrated displays for tank commanders and field laptop computers. We have the opportunity with this new technology to establish a common critical item product function specification for sunlight-readable, color and grayscale capable, flat panel displays for military applications. the Wright Laboratory is leading the development of such functional specification for U.S. military aircraft.

  1. Why SRS Matters - E Area

    SciTech Connect

    Howell, Steve; Mooneyhan, Verne; Tempel, Kevin; Bullington, Michele

    2015-03-09

    A video series presenting an overview of the Savannah River Site's (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode features E Area's mission and operations.

  2. Why SRS Matters - L Area

    SciTech Connect

    Hunt, Paul

    2015-01-28

    A video series presenting an overview of the Savannah River Site's (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode features L Area's mission and operations.

  3. Why SRS Matters - F Area

    SciTech Connect

    Howell, Steve; Tadlock, Bill; Beeler, Dewitt; Gardner, Curt

    2015-02-17

    A video series presenting an overview of the Savannah River Site's (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode features F Area's mission and operations.

  4. Why SRS Matters - H Canyon

    SciTech Connect

    Hunt, Paul; Lewczyk, Mike; Swain, Mike

    2015-02-17

    A video series presenting an overview of the Savannah River Site's (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode features H Canyon's mission and operations.

  5. Why SRS Matters - K Area

    SciTech Connect

    Hunt, Paul; Lawson, Janice

    2015-02-04

    A video series presenting an overview of the Savannah River Site (SRS) mission and operations. Each episode features a specific area/operation and how it contributes to help make the world safer. This episode features K Area's mission and operations.

  6. Srs2 and Mus81–Mms4 Prevent Accumulation of Toxic Inter-Homolog Recombination Intermediates

    PubMed Central

    Keyamura, Kenji; Arai, Kota

    2016-01-01

    Homologous recombination is an evolutionally conserved mechanism that promotes genome stability through the faithful repair of double-strand breaks and single-strand gaps in DNA, and the recovery of stalled or collapsed replication forks. Saccharomyces cerevisiae ATP-dependent DNA helicase Srs2 (a member of the highly conserved UvrD family of helicases) has multiple roles in regulating homologous recombination. A mutation (srs2K41A) resulting in a helicase-dead mutant of Srs2 was found to be lethal in diploid, but not in haploid, cells. In diploid cells, Srs2K41A caused the accumulation of inter-homolog joint molecule intermediates, increased the levels of spontaneous Rad52 foci, and induced gross chromosomal rearrangements. Srs2K41A lethality and accumulation of joint molecules were suppressed by inactivating Rad51 or deleting the Rad51-interaction domain of Srs2, whereas phosphorylation and sumoylation of Srs2 and its interaction with sumoylated proliferating cell nuclear antigen (PCNA) were not required for lethality. The structure-specific complex of crossover junction endonucleases Mus81 and Mms4 was also required for viability of diploid, but not haploid, SRS2 deletion mutants (srs2Δ), and diploid srs2Δ mus81Δ mutants accumulated joint molecule intermediates. Our data suggest that Srs2 and Mus81–Mms4 have critical roles in preventing the formation of (or in resolving) toxic inter-homolog joint molecules, which could otherwise interfere with chromosome segregation and lead to genetic instability. PMID:27390022

  7. 29 CFR 1918.52 - Specific requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 7 2013-07-01 2013-07-01 false Specific requirements. 1918.52 Section 1918.52 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Vessel's Cargo Handling Gear § 1918.52 Specific requirements. (a) Preventers. (1)...

  8. Requirements Specification Language (RSL) and supporting tools

    NASA Technical Reports Server (NTRS)

    Frincke, Deborah; Wolber, Dave; Fisher, Gene; Cohen, Gerald C.

    1992-01-01

    This document describes a general purpose Requirement Specification Language (RSL). RSL is a hybrid of features found in several popular requirement specification languages. The purpose of RSL is to describe precisely the external structure of a system comprised of hardware, software, and human processing elements. To overcome the deficiencies of informal specification languages, RSL includes facilities for mathematical specification. Two RSL interface tools are described. The Browser view contains a complete document with all details of the objects and operations. The Dataflow view is a specialized, operation-centered depiction of a specification that shows how specified operations relate in terms of inputs and outputs.

  9. Structured representation for requirements and specifications

    NASA Technical Reports Server (NTRS)

    Cohen, Gerald C.; Fisher, Gene; Frincke, Deborah; Wolber, Dave

    1991-01-01

    This document was generated in support of NASA contract NAS1-18586, Design and Validation of Digital Flight Control Systems suitable for Fly-By-Wire Applications, Task Assignment 2. Task 2 is associated with a formal representation of requirements and specifications. In particular, this document contains results associated with the development of a Wide-Spectrum Requirements Specification Language (WSRSL) that can be used to express system requirements and specifications in both stylized and formal forms. Included with this development are prototype tools to support the specification language. In addition a preliminary requirements specification methodology based on the WSRSL has been developed. Lastly, the methodology has been applied to an Advanced Subsonic Civil Transport Flight Control System.

  10. 14 CFR 221.40 - Specific requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) ECONOMIC REGULATIONS TARIFFS Contents of Tariff § 221.40 Specific requirements. (a) In addition to the... such service by manufacturer model designation and a description of the seating configuration...

  11. Technical requirements specification for tank waste retrieval

    SciTech Connect

    Lamberd, D.L.

    1996-09-26

    This document provides the technical requirements specification for the retrieval of waste from the underground storage tanks at the Hanford Site. All activities covered by this scope are conducted in support of the Tank Waste Remediation System (TWRS) mission.

  12. Testing of SRS and RFETS Nylon Bag Material

    SciTech Connect

    Laurinat, J.E.

    1998-11-03

    This report compares the effects of radiation and heating on nylon bagout materials used at the Savannah River Site (SRS) and the Rocky Flats Environmental Technology Site (RFETS). Recently, to simplify the processing of sand, slag, and crucible (SS and C), FB-Line has replaced the low-density polyethylene (LDPE) and polyvinyl chloride (PVC) bags normally used to package cans of plutonium-bearing material with nylon bags. LDPE and PVC are not soluble in the nitric acid dissolver solution used in F-Canyon, so cans bagged using these materials had to be repackaged before they were added to the dissolver. Because nylon dissolves in nitric acid, cans bagged in nylon can be charged to the F-Canyon dissolvers without repackaging, thereby reducing handling requirements and personnel exposure. As part of a program to process RFETS SS and C at SRS, RFETS has also begun to use a nylon bagout material. The RFETS bag materials is made from a copolymer of nylon 6 and nylon 6.9, while the SRS material is made from a nylon 6 monomer. In addition, the SRS nylon has an anti-static agent added. The RFETS nylon is slightly softer than the SRS nylon, but does not appear to be as resistant to flex cracks initiated by contact with sharp corners of the inner can containing the SS and C.2 FB-Line Operations has asked for measurement of the effects of radiation and heating on these materials. Specifically, they have requested a comparison of the material properties of the plastics before and after irradiation, a measurement of the amount of outgassing when the plastics are heated, and a calculation of the amount of radiolytic gas generation. Testing was performed on samples taken from material that is currently used in FB-Line (color coded orange) and at RFETS. The requested tests are the same tests previously performed on the original and replacement nylon and LDPE bag materials.3,4,5. To evaluate the effect of irradiation on material properties, tensile stresses and elongations to break

  13. 30 CFR 22.7 - Specific requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... APPROVAL OF MINING PRODUCTS PORTABLE METHANE DETECTORS § 22.7 Specific requirements. (a) Design. In the... shall be of such design that it will not produce sparks that will ignite an explosive mixture of methane and air. (5) Detectors of the flame type. Methane detectors of the flame type shall be subject to...

  14. 30 CFR 22.7 - Specific requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... APPROVAL OF MINING PRODUCTS PORTABLE METHANE DETECTORS § 22.7 Specific requirements. (a) Design. In the... shall be of such design that it will not produce sparks that will ignite an explosive mixture of methane and air. (5) Detectors of the flame type. Methane detectors of the flame type shall be subject to...

  15. Enterprise.SRS = Business for Success at SRS

    ScienceCinema

    None

    2016-07-12

    Goals and accomplishments of SRS. The debut of enterprise.srs, a strategic vision that will refocus site talents and efforts on developing future missions by broadening its impact in existing and new areas of national service. An expansion of people and facility in 3 areas: National Security, Clean Energy, and Environmental Stewardship.

  16. Enterprise.SRS = Business for Success at SRS

    SciTech Connect

    2011-01-01

    Goals and accomplishments of SRS. The debut of enterprise.srs, a strategic vision that will refocus site talents and efforts on developing future missions by broadening its impact in existing and new areas of national service. An expansion of people and facility in 3 areas: National Security, Clean Energy, and Environmental Stewardship.

  17. Handbook of methods for risk-based analysis of Technical Specification requirements

    SciTech Connect

    Samanta, P.K.; Vesely, W.E.

    1993-12-31

    Technical Specifications (TS) requirements for nuclear power plants define the Limiting Conditions for Operation (LCOs) and Surveillance Requirements (SRs) to assure safety during operation. In general, these requirements were based on deterministic analysis and engineering judgments. Experiences with plant operation indicate that some elements of the requirements are unnecessarily restrictive, while others may not be conducive to safety. Improvements in these requirements are facilitated by the availability of plant specific Probabilistic Safety Assessments (PSAs). The use of risk and reliability-based methods to improve TS requirements has gained wide interest because these methods can: quantitatively evaluate the risk impact and justify changes based on objective risk arguments. Provide a defensible basis for these requirements for regulatory applications. The United States Nuclear Regulatory Commission (USNRC) Office of Research is sponsoring research to develop systematic risk-based methods to improve various aspects of TS requirements. The handbook of methods, which is being prepared, summarizes such risk-based methods. The scope of the handbook includes reliability and risk-based methods for evaluating allowed outage times (AOTs), action statements requiring shutdown where shutdown risk may be substantial, surveillance test intervals (STIs), defenses against common-cause failures, managing plant configurations, and scheduling maintenances. For each topic, the handbook summarizes methods of analysis and data needs, outlines the insights to be gained, lists additional references, and presents examples of evaluations.

  18. SRS ECOLOGY ENVIRONMENTAL INFORMATION DOCUMENT

    SciTech Connect

    Wike, L; Doug Martin, D; Eric Nelson, E; Nancy Halverson, N; John Mayer, J; Michael Paller, M; Rodney Riley, R; Michael Serrato, M

    2006-03-01

    The SRS Ecology Environmental Information Document (EEID) provides a source of information on the ecology of Savannah River Site (SRS). The SRS is a U.S. Department of Energy (DOE)--owned property on the upper Atlantic Coastal Plain of South Carolina, centered approximately 40 kilometers (25 miles) southeast of Augusta, Georgia. The entire site was designated a National Environmental Research Park in 1972 by the Atomic Energy Commission, the predecessor of DOE. This document summarizes and synthesizes ecological research and monitoring conducted on the three main types of ecosystems found at SRS: terrestrial, wetland and aquatic. It also summarizes the available information on the threatened and endangered species found on the Savannah River Site. SRS is located along the Savannah River and encompasses an area of 80,267 hectares (310 square miles) in three South Carolina counties. It contains diverse habitats, flora, and fauna. Habitats include upland terrestrial areas, wetlands, streams, reservoirs, and the adjacent Savannah River. These diverse habitats support a variety of plants and animals, including many commercially or recreationally valuable species and several rare, threatened, or endangered species. Soils are the basic terrestrial resource, influencing the development of terrestrial biological communities. Many different soils exist on the SRS, from hydric to well-drained, and from sand to clay. In general, SRS soils are predominantly well-drained loamy sands.

  19. SRS: Site ranking system for hazardous chemical and radioactive waste

    SciTech Connect

    Rechard, R.P.; Chu, M.S.Y.; Brown, S.L.

    1988-05-01

    This report describes the rationale and presents instructions for a site ranking system (SRS). SRS ranks hazardous chemical and radioactive waste sites by scoring important and readily available factors that influence risk to human health. Using SRS, sites can be ranked for purposes of detailed site investigations. SRS evaluates the relative risk as a combination of potentially exposed population, chemical toxicity, and potential exposure of release from a waste site; hence, SRS uses the same concepts found in a detailed assessment of health risk. Basing SRS on the concepts of risk assessment tends to reduce the distortion of results found in other ranking schemes. More importantly, a clear logic helps ensure the successful application of the ranking procedure and increases its versatility when modifications are necessary for unique situations. Although one can rank sites using a detailed risk assessment, it is potentially costly because of data and resources required. SRS is an efficient approach to provide an order-of-magnitude ranking, requiring only readily available data (often only descriptive) and hand calculations. Worksheets are included to make the system easier to understand and use. 88 refs., 19 figs., 58 tabs.

  20. Integrated Bioinformatic and Targeted Deletion Analyses of the SRS Gene Superfamily Identify SRS29C as a Negative Regulator of Toxoplasma Virulence

    PubMed Central

    Wasmuth, James D.; Pszenny, Viviana; Haile, Simon; Jansen, Emily M.; Gast, Alexandra T.; Sher, Alan; Boyle, Jon P.; Boulanger, Martin J.; Parkinson, John; Grigg, Michael E.

    2012-01-01

    ABSTRACT The Toxoplasma gondii SRS gene superfamily is structurally related to SRS29B (formerly SAG1), a surface adhesin that binds host cells and stimulates host immunity. Comparative genomic analyses of three Toxoplasma strains identified 182 SRS genes distributed across 14 chromosomes at 57 genomic loci. Eight distinct SRS subfamilies were resolved. A core 69 functional gene orthologs were identified, and strain-specific expansions and pseudogenization were common. Gene expression profiling demonstrated differential expression of SRS genes in a developmental-stage- and strain-specific fashion and identified nine SRS genes as priority targets for gene deletion among the tissue-encysting coccidia. A Δsag1 ∆sag2A mutant was significantly attenuated in murine acute virulence and showed upregulated SRS29C (formerly SRS2) expression. Transgenic overexpression of SRS29C in the virulent RH parent was similarly attenuated. Together, these findings reveal SRS29C to be an important regulator of acute virulence in mice and demonstrate the power of integrated genomic analysis to guide experimental investigations. PMID:23149485

  1. Roadmap to the SRS computing architecture

    SciTech Connect

    Johnson, A.

    1994-07-05

    This document outlines the major steps that must be taken by the Savannah River Site (SRS) to migrate the SRS information technology (IT) environment to the new architecture described in the Savannah River Site Computing Architecture. This document proposes an IT environment that is {open_quotes}...standards-based, data-driven, and workstation-oriented, with larger systems being utilized for the delivery of needed information to users in a client-server relationship.{close_quotes} Achieving this vision will require many substantial changes in the computing applications, systems, and supporting infrastructure at the site. This document consists of a set of roadmaps which provide explanations of the necessary changes for IT at the site and describes the milestones that must be completed to finish the migration.

  2. General specifications covering requirements of aeronautic instruments

    NASA Technical Reports Server (NTRS)

    1917-01-01

    Report includes specifications for the use and production of instruments used in the navigation and operation of aircraft. Specifications are included for the following instruments: barometer or altimeter, compass, air speed meter, inclinometer, drift meter, tachometer, oil gauge, oil pressure gauge, gasoline gauge, gasoline flow indicator, distance indicator, barograph, angle of attack indicator, radiator temperature indicator, gasoline feed system pressure indicator, sextant, airplane director.

  3. MAXDOSE-SR: A routine release atmospheric dose model used at SRS

    SciTech Connect

    Simpkins, A.A.

    2000-02-09

    MAXDOSE-SR is a PC version of the dosimetry code MAXIGASP, which was used to calculate doses to the maximally exposed offsite individual for routine atmospheric releases of radioactive material at the Savannah River Site (SRS). Complete code description, verification of models, and user's manual have been included in this report. Minimal input is required to run the program, and site specific parameters are used when possible.

  4. SRS SLUDGE BATCH QUALIFICATION AND PROCESSING; HISTORICAL PERSPECTIVE AND LESSONS LEARNED

    SciTech Connect

    Cercy, M.; Peeler, D.; Stone, M.

    2013-09-25

    This report provides a historical overview and lessons learned associated with the SRS sludge batch (SB) qualification and processing programs. The report covers the framework of the requirements for waste form acceptance, the DWPF Glass Product Control Program (GPCP), waste feed acceptance, examples of how the program complies with the specifications, an overview of the Startup Program, and a summary of continuous improvements and lessons learned. The report includes a bibliography of previous reports and briefings on the topic.

  5. IMCS reflight certification requirements and design specifications

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The requirements for reflight certification are established. Software requirements encompass the software programs that are resident in the PCC, DEP, PDSS, EC, or any related GSE. A design approach for the reflight software packages is recommended. These designs will be of sufficient detail to permit the implementation of reflight software. The PDSS/IMC Reflight Certification system provides the tools and mechanisms for the user to perform the reflight certification test procedures, test data capture, test data display, and test data analysis. The system as defined will be structured to permit maximum automation of reflight certification procedures and test data analysis.

  6. Light duty utility arm software requirements specification

    SciTech Connect

    Kiebel, G.R.

    1995-12-18

    This document defines the software requirements for the integrated control and data acquisition system of the Light Duty Utility Arm (LDUA) System. It is intended to be used to guide the design of the application software, to be a basis for assessing the application software design, and to establish what is to be tested in the finished application software product.

  7. 30 CFR 22.7 - Specific requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... battery-type detectors is due chiefly to possible burning of the user by electrolyte that has spilled from the battery. MSHA, therefore, requires that: (1) Spilling of electrolyte. The battery shall be so designed and constructed that when properly filled it will not spill electrolyte under actual...

  8. 30 CFR 22.7 - Specific requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... battery-type detectors is due chiefly to possible burning of the user by electrolyte that has spilled from the battery. MSHA, therefore, requires that: (1) Spilling of electrolyte. The battery shall be so designed and constructed that when properly filled it will not spill electrolyte under actual...

  9. PDSS/IMC requirements and functional specifications

    NASA Technical Reports Server (NTRS)

    1983-01-01

    The system (software and hardware) requirements for the Payload Development Support System (PDSS)/Image Motion Compensator (IMC) are provided. The PDSS/IMC system provides the capability for performing Image Motion Compensator Electronics (IMCE) flight software test, checkout, and verification and provides the capability for monitoring the IMC flight computer system during qualification testing for fault detection and fault isolation.

  10. Hitchhiker: Customer Accommodations and Requirements Specifications (CARS)

    NASA Technical Reports Server (NTRS)

    1992-01-01

    In 1984, NASA Headquarters established projects at the Goddard Space Flight Center (GSFC) and the Marshall Space Flight Center (MSFC) to develop quick-reaction carrier systems for low-cost 'flight of opportunity' or secondary payloads on the Space Transportation System (STS). One of these projects is the Hitchhiker (HH) Program. GSFC has developed a family of carrier equipment known as the Shuttle Payload of Opportunity Carrier (SPOC) system for mounting small payloads such as HH to the side of the Orbiter payload bay. The side-mounted HHs are referred to as Hitchhiker-G (HH-G). MSFC developed a cross-bay 'bridge-type' carrier structure called the Hitchhiker-M (HH-M). In 1987, responsibility for the HH-M carrier was transferred to and is now managed by the HH Project Office at the GSFC. The HH-M carrier now uses the same interchangeable SPOC avionics unit and the same electrical interfaces and services developed for HH-G. National Aeronautics and Space Administration (NASA) has created this document to acquaint potential HH system customers with the facilities NASA provides and the requirements which customers must satisfy to use these facilities. This publication defines interface items required for integrating customer equipment with the HH carrier system. Those items such as mounting equipment and electrical inputs and outputs; configuration, environmental, command, telemetry, and operational constraints are described as well as weight, power, and communications. The purpose of this publication is to help the customer understand essential integration documentation requirements and to prepare a Customer Payload Requirements (CPR) document.

  11. Engineering Specifications derived from Science Requirements

    NASA Technical Reports Server (NTRS)

    Stahl, H. Philip; Arnold, William; Bevan, Ryan M.; Smith, W. Scott; Kirk, Charles S.; Postman, Marc

    2013-01-01

    Advanced Mirror Technology Development (AMTD) is a multi-year effort to systematically mature to TRL-6 the critical technologies needed to produce 4-m or larger flight-qualified UVOIR mirrors by 2018 so that a viable mission can be considered by the 2020 Decadal Review. This technology must enable missions capable of both general astrophysics & ultra-high contrast observations of exoplanets. To accomplish our objective, we use a science-driven systems engineering approach. We mature technologies required to enable the highest priority science AND result in a high-performance low-cost low-risk system.

  12. 46 CFR 63.20-1 - Specific control system requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 2 2014-10-01 2014-10-01 false Specific control system requirements. 63.20-1 Section 63.20-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC AUXILIARY BOILERS Additional Control System Requirements § 63.20-1 Specific control system requirements. In addition to the requirements found...

  13. SRS ecology: Environmental information document

    SciTech Connect

    Wike, L.D.; Shipley, R.W.; Bowers, J.A.

    1993-09-01

    The purpose of this Document is to provide a source of ecological information based on the exiting knowledge gained from research conducted at the Savannah River Site. This document provides a summary and synthesis of ecological research in the three main ecosystem types found at SRS and information on the threatened and endangered species residing there.

  14. Structuring Formal Requirements Specifications for Reuse and Product Families

    NASA Technical Reports Server (NTRS)

    Heimdahl, Mats P. E.

    2001-01-01

    In this project we have investigated how formal specifications should be structured to allow for requirements reuse, product family engineering, and ease of requirements change, The contributions of this work include (1) a requirements specification methodology specifically targeted for critical avionics applications, (2) guidelines for how to structure state-based specifications to facilitate ease of change and reuse, and (3) examples from the avionics domain demonstrating the proposed approach.

  15. RADIOIODINE GEOCHEMISTRY IN THE SRS SUBSURFACE ENVIRONMENT

    SciTech Connect

    Kaplan, D.; Emerson, H.; Powell, B.; Roberts, K.; Zhang, S.; Xu, C.; Schwer, K.; Li, H.; Ho, Y.; Denham, M.; Yeager, C.; Santschi, P.

    2013-05-16

    Iodine-129 is one of the key risk drivers for several Savannah River Site (SRS) performance assessments (PA), including that for the Low-Level Waste Disposal Facility in E-Area. In an effort to reduce the uncertainty associated with the conceptual model and the input values used in PA, several studies have recently been conducted dealing with radioiodine geochemistry at the SRS. The objective of this report was to review these recent studies and evaluate their implications on SRS PA calculations. For the first time, these studies measured iodine speciation in SRS groundwater and provided technical justification for assuming the presence of more strongly sorbing species (iodate and organo-iodine), and measured greater iodine sediment sorption when experiments included these newly identified species; specifically they measured greater sorption coefficients (K{sub d} values: the concentration ratio of iodine on the solid phase divided by the concentration in the aqueous phase). Based on these recent studies, new best estimates were proposed for future PA calculations. The new K{sub d} values are greater than previous recommended values. These proposed K{sub d} values reflect a better understanding of iodine geochemistry in the SRS subsurface environment, which permits reducing the associated conservatism included in the original estimates to account for uncertainty. Among the key contributing discoveries supporting the contention that the K{sub d} values should be increased are that: 1) not only iodide (I{sup -}), but also the more strongly sorbing iodate (IO{sub 3}{sup -}) species exists in SRS groundwater (average total iodine = 15% iodide, 42% iodate, and 43% organoiodine), 2) when iodine was added as iodate, the measured K{sub d} values were 2 to 6 times greater than when the iodine was added as iodide, and perhaps most importantly, 3) higher desorption (10 to 20 mL/g) than (ad)sorption (all previous studies) K{sub d} values were measured. The implications of this

  16. 49 CFR 178.35 - General requirements for specification cylinders.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS... perform the following: (1) Inspect all material and reject any not meeting applicable requirements. For... and cold break. (2) Verify the material of construction meets the requirements of the...

  17. 49 CFR 178.35 - General requirements for specification cylinders.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS... perform the following: (1) Inspect all material and reject any not meeting applicable requirements. For... and cold break. (2) Verify the material of construction meets the requirements of the...

  18. RADIOLYTIC HYDROGEN GENERATION INSAVANNAH RIVER SITE (SRS) HIGH LEVEL WASTETANKS COMPARISON OF SRS AND HANFORDMODELING PREDICTIONS

    SciTech Connect

    Crawford, C; Ned Bibler, N

    2009-04-15

    the waste. The difference in the two models has led to the questions of how different are the results predicted by the two models and which model predicts the more conservative (larger) G values. More conservative G values would predict higher H{sub 2} generation rates that would require higher ventilation rates in the SRS tanks. This report compares predictions based on the two models at various nitrate and nitrite concentrations in the SRS HLW tanks for both beta/gamma and for alpha radiation. It also compares predicted G values with those determined by actually measuring the H{sub 2} production from four SRS HLW tanks (Tanks 32H, 35H, 39H, and 42H). Lastly, the H{sub 2} generation rates predicted by the two models are compared for the 47 active SRS high level waste tanks using the most recent tank nitrate and nitrite concentrations and the beta/gamma and alpha heat loads for each tank. The predictions of the models for total H{sub 2} generation rates from the 47 active SRS waste were, for the most part, similar. For example, the predictions for both models applied to 25 tanks agreed within {+-}10% of each other. For the remaining 22 tanks, the SRS prediction was more conservative for 9 tanks (maximum 29% higher) and the Hanford prediction was more conservative for 13 tanks (maximum 19% higher). When comparing G values predicted by the equations presuming only alpha radiation or only beta/gamma was present the results were somewhat different. The results of predictions for alpha radiation, at the 47 current nitrate and nitrite concentrations in the SRS tanks indicated that all the SRS predictions were higher (up to 30%) than the Hanford predictions and thus more conservative. For beta/gamma radiation the predictions for both models agreed to {+-}10% for 18 of the combinations, the Hanford model predicted higher values (11 up to 17%) for 25 of the concentrations considered, and the SRS model predicted higher G values for the remaining two combinations (12 and 17

  19. 49 CFR 173.62 - Specific packaging requirements for explosives.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Federal Register citations affecting § 173.62, see the List of CFR Sections Affected, which appears in the... 49 Transportation 2 2014-10-01 2014-10-01 false Specific packaging requirements for explosives... Class 1 § 173.62 Specific packaging requirements for explosives. (a) Except as provided in § 173.7...

  20. 49 CFR 173.62 - Specific packaging requirements for explosives.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... List of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and at... 49 Transportation 2 2013-10-01 2013-10-01 false Specific packaging requirements for explosives... Class 1 § 173.62 Specific packaging requirements for explosives. (a) Except as provided in § 173.7...

  1. 49 CFR 173.62 - Specific packaging requirements for explosives.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... citations affecting § 173.62, see the List of CFR Sections Affected, which appears in the Finding Aids... 49 Transportation 2 2011-10-01 2011-10-01 false Specific packaging requirements for explosives... Class 1 § 173.62 Specific packaging requirements for explosives. (a) Except as provided in § 173.7...

  2. 49 CFR 173.62 - Specific packaging requirements for explosives.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... citations affecting § 173.62, see the List of CFR Sections Affected, which appears in the Finding Aids... 49 Transportation 2 2012-10-01 2012-10-01 false Specific packaging requirements for explosives... Class 1 § 173.62 Specific packaging requirements for explosives. (a) Except as provided in § 173.7...

  3. 29 CFR 1926.651 - Specific excavation requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 8 2014-07-01 2014-07-01 false Specific excavation requirements. 1926.651 Section 1926.651..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR CONSTRUCTION Excavations § 1926.651 Specific excavation requirements. (a) Surface encumbrances. All surface encumbrances that are located so...

  4. From Goal-Oriented Requirements to Event-B Specifications

    NASA Technical Reports Server (NTRS)

    Aziz, Benjamin; Arenas, Alvaro E.; Bicarregui, Juan; Ponsard, Christophe; Massonet, Philippe

    2009-01-01

    In goal-oriented requirements engineering methodologies, goals are structured into refinement trees from high-level system-wide goals down to fine-grained requirements assigned to specific software/ hardware/human agents that can realise them. Functional goals assigned to software agents need to be operationalised into specification of services that the agent should provide to realise those requirements. In this paper, we propose an approach for operationalising requirements into specifications expressed in the Event-B formalism. Our approach has the benefit of aiding software designers by bridging the gap between declarative requirements and operational system specifications in a rigorous manner, enabling powerful correctness proofs and allowing further refinements down to the implementation level. Our solution is based on verifying that a consistent Event-B machine exhibits properties corresponding to requirements.

  5. Electrical Bonding: A Survey of Requirement, Methods, and Specifications

    NASA Technical Reports Server (NTRS)

    Evans, R. W.

    1998-01-01

    This document provides information helpful to engineers imposing electrical bonding requirements, reviewing waiver requests, or modifying specifications on various space programs. Electrical bonding specifications and some of the processes used in the United States have been reviewed. This document discusses the specifications, the types of bonds, the intent of each, and the basic requirements where possible. Additional topics discussed are resistance versus impedance, bond straps, corrosion, finishes, and special applications.

  6. SRS tank closure. Innovative technology summary report

    SciTech Connect

    Not Available

    1999-08-01

    High-level waste (HLW) tank closure technology is designed to stabilize any remaining radionuclides and hazardous constituents left in a tank after bulk waste removal. Two Savannah River Site (SRS) HLW tanks were closed after cleansing and then filling each tank with three layers of grout. The first layer consists of a chemically reducing grout. The fill material has chemical properties that retard the movement of some radionuclides and chemical constituents. A layer of controlled low-strength material (CLSM), a self-leveling fill material, is placed on top of the reducing grout. CLSM provides sufficient strength to support the overbearing weight. The final layer is a free-flowing, strong grout similar to normal concrete. After the main tank cavity is filled, risers are filled with grout, and all waste transfer piping connected to the tank is isolated. The tank ventilation system is dismantled, and the remaining systems are isolated. Equipment that remains with the tank is filled with grout. The tank and ancillary systems are left in a state requiring only limited surveillance. Administrative procedures are in place to control land use and access. DOE eventually plans to remove all of its HLW storage tanks from service. These tanks are located at SRS, Hanford, and Idaho National Engineering and Environmental Laboratory. Low-activity waste storage tanks at Oak Ridge Reservation are also scheduled for closure.

  7. Methodology for Estimating Ingestion Dose for Emergency Response at SRS

    SciTech Connect

    Simpkins, A.A.

    2003-07-21

    At the Savannah River Site (SRS), emergency response computer models are used to estimate dose following releases of radioactive materials to the environment. Downwind air and ground concentrations and their associated doses from inhalation and ground shine pathways are estimated. The emergency response model (PUFF-PLUME) uses real-time data to track either instantaneous (puff) or continuous (plume) releases. A site-specific ingestion dose model was developed for use with PUFF-PLUME that includes the following ingestion dose pathways pertinent to the surrounding SRS area: milk, beef, water, and fish. The model is simplistic and can be used with existing code output.

  8. Technical Review of SRS Dose Reconstrruction Methods Used By CDC

    SciTech Connect

    Simpkins, Ali, A

    2005-07-20

    At the request of the Centers for Disease Control and Prevention (CDC), a subcontractor Advanced Technologies and Laboratories International, Inc.(ATL) issued a draft report estimating offsite dose as a result of Savannah River Site operations for the period 1954-1992 in support of Phase III of the SRS Dose Reconstruction Project. The doses reported by ATL differed than those previously estimated by Savannah River Site SRS dose modelers for a variety of reasons, but primarily because (1) ATL used different source terms, (2) ATL considered trespasser/poacher scenarios and (3) ATL did not consistently use site-specific parameters or correct usage parameters. The receptors with the highest dose from atmospheric and liquid pathways were within about a factor of four greater than dose values previously reported by SRS. A complete set of technical comments have also been included.

  9. UML activity diagrams in requirements specification of logic controllers

    NASA Astrophysics Data System (ADS)

    Grobelna, Iwona; Grobelny, Michał

    2015-12-01

    Logic controller specification can be prepared using various techniques. One of them is the wide understandable and user-friendly UML language and its activity diagrams. Using formal methods during the design phase increases the assurance that implemented system meets the project requirements. In the approach we use the model checking technique to formally verify a specification against user-defined behavioral requirements. The properties are usually defined as temporal logic formulas. In the paper we propose to use UML activity diagrams in requirements definition and then to formalize them as temporal logic formulas. As a result, UML activity diagrams can be used both for logic controller specification and for requirements definition, what simplifies the specification and verification process.

  10. 47 CFR 76.75 - Specific EEO program requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Equal Employment Opportunity Requirements § 76.75 Specific EEO... necessary. Nothing in this section shall be interpreted to require a multichannel video programming...) In addition to using such recruitment sources, a multichannel video programming...

  11. 47 CFR 76.75 - Specific EEO program requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Equal Employment Opportunity Requirements § 76.75 Specific EEO... necessary. Nothing in this section shall be interpreted to require a multichannel video programming...) In addition to using such recruitment sources, a multichannel video programming...

  12. 47 CFR 76.75 - Specific EEO program requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Equal Employment Opportunity Requirements § 76.75 Specific EEO... necessary. Nothing in this section shall be interpreted to require a multichannel video programming...) In addition to using such recruitment sources, a multichannel video programming...

  13. A Framework for RFID Survivability Requirement Analysis and Specification

    NASA Astrophysics Data System (ADS)

    Zuo, Yanjun; Pimple, Malvika; Lande, Suhas

    Many industries are becoming dependent on Radio Frequency Identification (RFID) technology for inventory management and asset tracking. The data collected about tagged objects though RFID is used in various high level business operations. The RFID system should hence be highly available, reliable, and dependable and secure. In addition, this system should be able to resist attacks and perform recovery in case of security incidents. Together these requirements give rise to the notion of a survivable RFID system. The main goal of this paper is to analyze and specify the requirements for an RFID system to become survivable. These requirements, if utilized, can assist the system in resisting against devastating attacks and recovering quickly from damages. This paper proposes the techniques and approaches for RFID survivability requirements analysis and specification. From the perspective of system acquisition and engineering, survivability requirement is the important first step in survivability specification, compliance formulation, and proof verification.

  14. SRS Waste Characterization System Database

    SciTech Connect

    Hester, J.R.

    2000-03-02

    Information relating to the contents of Savannah River Site high level waste tanks is available from numerous and scattered sources. The Waste Characterization System (WCS) database consolidates this information and provides for the orderly weighing of contradictory information. WCS is a computerized system that tracks the inventory of selected chemicals and radionuclides in high level waste tanks. WCS resides on an SRS local network server. WCS inventories cover approximately ninety non-radioactive chemicals and forty radionuclides and are based on concentration estimates from sample analyses, process histories, composition studies, and theoretical relationships. The intent of WCS is to consolidate waste characterization information, which now exists in multiple locations, and to estimate the most likely values of waste constituent inventories, which are based on the best-estimate compositions.

  15. Phase matching of four-wave interactions of SRS components in birefringent SRS-active crystals

    NASA Astrophysics Data System (ADS)

    Smetanin, Sergei N.; Basiev, Tasoltan T.

    2012-03-01

    A new method has been proposed for achieving wave vector matching in four-wave interactions of frequency components upon SRS in birefringent SRS-active crystals. The method ensures anti-Stokes wave generation and enables a substantial reduction in higher order Stokes SRS generation thresholds. Phase matching directions in BaWO4 SRS-active negative uniaxial crystals and SrWO4 SRS-active positive uniaxial crystals have been found in the wavelength range 0.4 — 0.7 μm.

  16. Commercial Submersible Mixing Pump For SRS Tank Waste Removal - 15223

    SciTech Connect

    Hubbard, Mike; Herbert, James E.; Scheele, Patrick W.

    2015-01-12

    The Savannah River Site Tank Farms have 45 active underground waste tanks used to store and process nuclear waste materials. There are 4 different tank types, ranging in capacity from 2839 m3 to 4921 m3 (750,000 to 1,300,000 gallons). Eighteen of the tanks are older style and do not meet all current federal standards for secondary containment. The older style tanks are the initial focus of waste removal efforts for tank closure and are referred to as closure tanks. Of the original 51 underground waste tanks, six of the original 24 older style tanks have completed waste removal and are filled with grout. The insoluble waste fraction that resides within most waste tanks at SRS requires vigorous agitation to suspend the solids within the waste liquid in order to transfer this material for eventual processing into glass filled canisters at the Defense Waste Processing Facility (DWPF). SRS suspends the solid waste by use of recirculating mixing pumps. Older style tanks generally have limited riser openings which will not support larger mixing pumps, since the riser access is typically 58.4 cm (23 inches) in diameter. Agitation for these tanks has been provided by four long shafted standard slurry pumps (SLP) powered by an above tank 112KW (150 HP) electric motor. The pump shaft is lubricated and cooled in a pressurized water column that is sealed from the surrounding waste in the tank. Closure of four waste tanks has been accomplished utilizing long shafted pump technology combined with heel removal using multiple technologies. Newer style waste tanks at SRS have larger riser openings, allowing the processing of waste solids to be accomplished with four large diameter SLPs equipped with 224KW (300 HP) motors. These tanks are used to process the waste from closure tanks for DWPF. In addition to the SLPs, a 224KW (300 HP) submersible mixer pump (SMP) has also been developed and deployed within older style tanks. The SMPs are product cooled and

  17. Independent verification and validation of large software requirement specification databases

    SciTech Connect

    Twitchell, K.E.

    1992-04-01

    To enhance quality, an independent verification and validation (IV V) review is conducted as software requirements are defined. Requirements are inspected for consistency and completeness. IV V strives to detect defects early in the software development life cycle and to prevent problems before they occur. The IV V review process of a massive software requirements specification, the Reserve Component Automation System (RCAS) Functional Description (FD) is explored. Analysis of the RCAS FD error history determined that there are no predictors of errors. The size of the FD mandates electronic analysis of the databases. Software which successfully performs automated consistency and completeness checks is discussed. The process of verifying the quality of analysis software is described. The use of intuitive ad hoc techniques, in addition to the automatic analysis of the databases, is required because of the varying content of the requirements databases. The ad hoc investigation process is discussed. Case studies are provided to illustrate how the process works. This thesis demonstrates that it is possible to perform an IV V review on a massive software requirements specification. Automatic analysis enables inspecting for completeness and consistency. The work with the RCAS FD clearly indicates that the IV V review process is not static; it must continually grow, adapt, and change as conditions warrant. The ad hoc investigation process provides this required flexibility This process also analyzes errors discovered by manual review and automatic processing. The analysis results in the development of new algorithms and the addition of new programs to the automatic inspection software.

  18. Precise time and time interval users, requirements and specifications

    NASA Technical Reports Server (NTRS)

    Bowser, J. R.

    1982-01-01

    The functional areas of application of Precise Time and Time Interval (PTTI) were considered and expanded. A comprehensive overview of the PTTI requirements and applications would provide an opportunity for individuals working in a specific functional area. Mutual problems, requirements, applications or successes shared by those in other functional areas were studied. Based upon the results of a two year study a compendium of PTTI requirements, applications and the means of meeting the requirements among Department of Defense components, other government agencies and major commercial users was compiled and is presented. It was found that the planning process for PTTI support for new acquisitions or new programs was less than a well defined, coordinated process. The processes are described in general terms and a generic model for requirements determination and subsequent coordination which may enhance the planning process and introduce cost benefits to the program is also presented.

  19. 49 CFR 178.35 - General requirements for specification cylinders.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...) Inspections and analyses. Chemical analyses and tests required by this subchapter must be made within the... cylinders made by the billet-piercing process, billets must be inspected and shown to be free from pipe... specification by— (i) Making a chemical analysis of each heat of material; (ii) Obtaining a certified...

  20. 19 CFR 134.42 - Specific method may be required.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Specific method may be required. 134.42 Section 134.42 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY COUNTRY OF ORIGIN MARKING Method and Location of Marking Imported Articles §...

  1. 24 CFR 1003.302 - Project specific threshold requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Project specific threshold requirements. 1003.302 Section 1003.302 Housing and Urban Development Regulations Relating to Housing and Urban... URBAN DEVELOPMENT COMMUNITY DEVELOPMENT BLOCK GRANTS FOR INDIAN TRIBES AND ALASKA NATIVE VILLAGES...

  2. 40 CFR 86.1506 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Gas-Fueled Diesel-Cycle Heavy-Duty Engines, New Otto-Cycle Light-Duty Trucks, and New Methanol-Fueled... trucks. Equipment required and specifications are as follows: (1) Exhaust emission tests. All engines and... PROGRAMS (CONTINUED) CONTROL OF EMISSIONS FROM NEW AND IN-USE HIGHWAY VEHICLES AND ENGINES...

  3. 18 CFR 12.35 - Specific inspection requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Specific inspection requirements. 12.35 Section 12.35 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT SAFETY OF WATER POWER PROJECTS...

  4. 18 CFR 12.35 - Specific inspection requirements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Specific inspection requirements. 12.35 Section 12.35 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT SAFETY OF WATER POWER PROJECTS...

  5. 18 CFR 12.35 - Specific inspection requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Specific inspection requirements. 12.35 Section 12.35 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT SAFETY OF WATER POWER PROJECTS...

  6. 18 CFR 12.35 - Specific inspection requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Specific inspection requirements. 12.35 Section 12.35 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT SAFETY OF WATER POWER PROJECTS...

  7. 18 CFR 12.35 - Specific inspection requirements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Specific inspection requirements. 12.35 Section 12.35 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT SAFETY OF WATER POWER PROJECTS...

  8. Information Requirements Specification II: Brainstorming Collective Decision-Making Technique.

    ERIC Educational Resources Information Center

    Telem, Moshe

    1988-01-01

    Information requirements specification (IRS) constitutes an Achilles heel in the system life cycle of management information systems. This article establishes a systematic overall IRS technique applicable to organizations of all types and sizes. The technique's integration of brainstorming and theory Z principles creates an effective, stimulating,…

  9. Draft Geologic Disposal Requirements Basis for STAD Specification

    SciTech Connect

    Ilgen, Anastasia G.; Bryan, Charles R.; Hardin, Ernest

    2015-03-25

    This document provides the basis for requirements in the current version of Performance Specification for Standardized Transportation, Aging, and Disposal Canister Systems, (FCRD-NFST-2014-0000579) that are driven by storage and geologic disposal considerations. Performance requirements for the Standardized Transportation, Aging, and Disposal (STAD) canister are given in Section 3.1 of that report. Here, the requirements are reviewed and the rationale for each provided. Note that, while FCRD-NFST-2014-0000579 provides performance specifications for other components of the STAD storage system (e.g. storage overpack, transfer and transportation casks, and others), these have no impact on the canister performance during disposal, and are not discussed here.

  10. Update on requirements and specifications for ignition experiments on NIF

    NASA Astrophysics Data System (ADS)

    Haan, Steven

    2012-10-01

    The National Ignition Campaign (NIC) on the National Ignition Facility plans to use an indirectly driven spherical implosion to assemble and ignite a mass of DT fuel. Requirements describing the specifics of the ignition implosion and the corresponding expected performance were established several years prior. These requirements include laser features, target fabrication and characterization, and data obtained from pre-ignition experiments. Since those requirements were originally set, the NIC has conducted a variety of experiments using surrogate targets, meant to define various aspects of the future ignition experiment. Results from this experimental campaign, as well as progress in understanding the laser performance and issues raised by target fabrication, have motivated updates to the target designs and pre-established requirements. A summary of these updates, and their implications for ignition campaign planning, will be presented.

  11. Software requirements: Guidance and control software development specification

    NASA Technical Reports Server (NTRS)

    Withers, B. Edward; Rich, Don C.; Lowman, Douglas S.; Buckland, R. C.

    1990-01-01

    The software requirements for an implementation of Guidance and Control Software (GCS) are specified. The purpose of the GCS is to provide guidance and engine control to a planetary landing vehicle during its terminal descent onto a planetary surface and to communicate sensory information about that vehicle and its descent to some receiving device. The specification was developed using the structured analysis for real time system specification methodology by Hatley and Pirbhai and was based on a simulation program used to study the probability of success of the 1976 Viking Lander missions to Mars. Three versions of GCS are being generated for use in software error studies.

  12. Rationale for windshield glass system specification requirements for shuttle orbiter

    NASA Technical Reports Server (NTRS)

    Hayashida, K.; King, G. L.; Tesinsiky, J.; Wittenburg, D. R.

    1972-01-01

    A preliminary procurement specification for the space shuttle orbiter windshield pane, and some of the design considerations and rationale leading to its development are presented. The windshield designer is given the necessary methods and procedures for assuring glass pane structural integrity by proof test. These methods and procedures are fully developed for annealed and thermally tempered aluminosilicate, borosilicate, and soda lime glass and for annealed fused silica. Application of the method to chemically tempered glass is considered. Other considerations are vision requirements, protection against bird impact, hail, frost, rain, and meteoroids. The functional requirements of the windshield system during landing, ferrying, boost, space flight, and entry are included.

  13. Independent verification and validation of large software requirement specification databases

    SciTech Connect

    Twitchell, K.E.

    1992-04-01

    To enhance quality, an independent verification and validation (IV&V) review is conducted as software requirements are defined. Requirements are inspected for consistency and completeness. IV&V strives to detect defects early in the software development life cycle and to prevent problems before they occur. The IV&V review process of a massive software requirements specification, the Reserve Component Automation System (RCAS) Functional Description (FD) is explored. Analysis of the RCAS FD error history determined that there are no predictors of errors. The size of the FD mandates electronic analysis of the databases. Software which successfully performs automated consistency and completeness checks is discussed. The process of verifying the quality of analysis software is described. The use of intuitive ad hoc techniques, in addition to the automatic analysis of the databases, is required because of the varying content of the requirements databases. The ad hoc investigation process is discussed. Case studies are provided to illustrate how the process works. This thesis demonstrates that it is possible to perform an IV&V review on a massive software requirements specification. Automatic analysis enables inspecting for completeness and consistency. The work with the RCAS FD clearly indicates that the IV&V review process is not static; it must continually grow, adapt, and change as conditions warrant. The ad hoc investigation process provides this required flexibility This process also analyzes errors discovered by manual review and automatic processing. The analysis results in the development of new algorithms and the addition of new programs to the automatic inspection software.

  14. Analyzing Tabular and State-Transition Requirements Specifications in PVS

    NASA Technical Reports Server (NTRS)

    Owre, Sam; Rushby, John; Shankar, Natarajan

    1997-01-01

    We describe PVS's capabilities for representing tabular specifications of the kind advocated by Parnas and others, and show how PVS's Type Correctness Conditions (TCCs) are used to ensure certain well-formedness properties. We then show how these and other capabilities of PVS can be used to represent the AND/OR tables of Leveson and the Decision Tables of Sherry, and we demonstrate how PVS's TCCs can expose and help isolate errors in the latter. We extend this approach to represent the mode transition tables of the Software Cost Reduction (SCR) method in an attractive manner. We show how PVS can check these tables for well-formedness, and how PVS's model checking capabilities can be used to verify invariants and reachability properties of SCR requirements specifications, and inclusion relations between the behaviors of different specifications. These examples demonstrate how several capabilities of the PVS language and verification system can be used in combination to provide customized support for specific methodologies for documenting and analyzing requirements. Because they use only the standard capabilities of PVS, users can adapt and extend these customizations to suit their own needs. Those developing dedicated tools for individual methodologies may find these constructions in PVS helpful for prototyping purposes, or as a useful adjunct to a dedicated tool when the capabilities of a full theorem prover are required. The examples also illustrate the power and utility of an integrated general-purpose system such as PVS. For example, there was no need to adapt or extend the PVS model checker to make it work with SCR specifications described using the PVS TABLE construct: the model checker is applicable to any transition relation, independently of the PVS language constructs used in its definition.

  15. Requirements specification for nickel cadmium battery expert system

    NASA Technical Reports Server (NTRS)

    1986-01-01

    The requirements for performance, design, test, and qualification of a computer program identified as NICBES, Nickel Cadmium Battery Expert System, is established. The specific spacecraft power system configuration selected was the Hubble Space Telescope (HST) Electrical Power System (EPS) Testbed. Power for the HST comes from a system of 13 Solar Panel Arrays (SPAs) linked to 6 Nickel Cadmium Batteries which are connected to 3 Busses. An expert system, NICBES, will be developed at Martin Marietta Aerospace to recognize a testbed anomaly, identify the malfunctioning component and recommend a course of action. Besides fault diagnosis, NICBES will be able to evaluate battery status, give advice on battery status and provide decision support for the operator. These requirements are detailed.

  16. Requirements specification for automated fall and injury risk assessment.

    PubMed

    Currie, Leanne M; Mellino, Lourdes V; Cimino, James J; Li, Jianhua; Bakken, Suzanne

    2006-01-01

    Fall and injury prevention continues to be a challenge in the acute care environment. Identification of patients at risk can guide preventive care for these individuals. The following study employed usability engineering methods via a series of focus groups, to specify functional and design requirements for an automated Fall-Injury Risk Assessment Instrument. Focus groups were held with interdisciplinary decision makers and end-users to identify functional and design specifications for the automated instrument. The results were mapped to usability heuristics, which were used to guide design decisions. The main elements identified were data completeness, workflow processes, resource access, and cognitive burden. The main usability factors identified were efficiency of user, match with real world, error prevention, recognition not recall and minimalist design. Focus groups are a useful methodology to specify requirements for healthcare applications. Outcomes evaluation of the automated instrument is in process. PMID:17102234

  17. Incubator Display Software Cost Reduction Toolset Software Requirements Specification

    NASA Technical Reports Server (NTRS)

    Moran, Susanne; Jeffords, Ralph

    2005-01-01

    The Incubator Display Software Requirements Specification was initially developed by Intrinsyx Technologies Corporation (Intrinsyx) under subcontract to Lockheed Martin, Contract Number NAS2-02090, for the National Aeronautics and Space Administration (NASA) Ames Research Center (ARC) Space Station Biological Research Project (SSBRP). The Incubator Display is a User Payload Application (UPA) used to control an Incubator subrack payload for the SSBRP. The Incubator Display functions on-orbit as part of the subrack payload laptop, on the ground as part of the Communication and Data System (CDS) ground control system, and also as part of the crew training environment.

  18. Energy storage specification requirements for hybrid-electric vehicle

    SciTech Connect

    Burke, A.F.

    1993-09-01

    A study has been made of energy storage unit requirements for hybrid-electric vehicles. The drivelines for these vehicles included both primary energy storage units and/or pulse power units. The primary energy storage units were sized to provide ``primary energy`` ranges up to 60 km. The total power capability of the drivelines were such that the vehicles had 0 to 100 km/h acceleration times of 10 to 12 s. The power density requirements for primary energy storage devices to be used in hybrid vehicles are much higher than that for devices to be used in electric vehicles. The energy density and power density requirements for pulse-power devices for hybrid vehicles, are not much different than those in an electric vehicle. The cycle life requirements for primary energy-storage units for hybrid vehicles are about double that for electric vehicles, because of the reduced size of the storage units in the hybrid vehicles. The cycle life for pulse-power devices for hybrid vehicles is about the same as for electric vehicles having battery load leveling. Because of the need for additional components in the hybrid driveline, the cost of the energy storage units in hybrid vehicles should be much less (at least a factor of two) than those in electric vehicles. There are no presently available energy storage units that meet all the specifications for hybrid vehicle applications, but ultracapacitors and bipolar lead-acid batteries are under development that have the potential for meeting them. If flywheel systems having a mechanical system energy density of 40 to 50 W{center_dot}h/kg and an electrical system power density of 2 to 3 kw/kg can be developed, they would have the potential of meeting specifications for primary storage and pulse power units.

  19. ANALYSIS OF SPECIAL WASTE CONFIGURATIONS AT THE SRS WASTE MANAGEMENT FACILITIES

    SciTech Connect

    Casella, V; Raymond Dewberry, R

    2007-05-14

    Job Control Waste (JCW) at the Savannah River Site (SRS) Solid Waste Management Facilities (SWMF) may be disposed of in special containers, and the analysis of these containers requires developing specific analysis methodologies. A method has been developed for the routine assay of prohibited items (liquids, etc.) contained in a 30-gallon drum that is then placed into a 55-gallon drum. Method development consisted of system calibration with a NIST standard at various drum-to-detector distances, method verification with a liquid sample containing a known amount of Pu-238, and modeling the inner container using Ortec Isotopic software. Using this method for measurement of the known standard in the drum-in-drum configuration produced excellent agreement (within 15%) with the known value. Savannah River Site Solid Waste Management also requested analysis of waste contained in large black boxes (commonly 18-feet x 12-feet x 7-feet) stored at the SWMF. These boxes are frequently stored in high background areas and background radiation must be considered for each analysis. A detection limit of less than 150 fissile-gram-equivalents (FGE) of TRU waste is required for the black-box analyses. There is usually excellent agreement for the measurements at different distances and measurement uncertainties of about 50% are obtained at distances of at least twenty feet from the box. This paper discusses the experimental setup, analysis and data evaluation for drum-in-drum and black box waste configurations at SRS.

  20. SRS Computer Animation and Drive Train System

    NASA Technical Reports Server (NTRS)

    Arthun, Daniel; Schachner, Christian

    2001-01-01

    The spinning rocket simulator (SRS) is an ongoing project at Oral Roberts University. The goal of the SRS is to gather crucial data concerning a spinning rocket under thrust for the purpose of analysis and correction of the coning motion experienced by this type of spacecraft maneuver. The computer animation simulates a virtual, scale model of the component of the SRS that represents the spacecraft itself. This component is known as the (VSM), or virtual spacecraft model. During actual physical simulation, this component of the SRS will experience a coning. The goal of the animation is to cone the VSM within that range to accurately represent the motion of the actual simulator. The drive system of the SRS is the apparatus that turns the actual simulator. It consists of a drive motor, motor mount and chain to power the simulator into motion. The motor mount is adjustable and rigid for high torque application. A digital stepper motor controller actuates the main drive motor for linear acceleration. The chain transfers power from the motor to the simulator via sprockets on both ends.

  1. Savannah River Site (SRS) environmental overview

    SciTech Connect

    O'Rear, M.G. ); Steele, J.L.; Kitchen, B.G. )

    1990-01-01

    The environmental surveillance activities at and in the vicinity of the Savannah River Site (SRS) (formerly the Savannah River Plant (SRP)) comprise one of the most comprehensive and extensive environmental monitoring programs in the United States. This overview contains monitoring data from routine and nonroutine radiological and nonradiological environmental surveillance activities, summaries of environmental protection programs in progress, a summary of National Environmental Policy Act (NEPA) activities, and a listing of environmental permits (Appendix A) issued by regulatory agencies. This overview provides information about the impact of SRS operations on the public and the environment. The SRS occupies a large area of approximately 300 square miles along the Savannah River, principally in Aiken and Barnwell counties of South Carolina. SRS's primary function is the production of tritium, plutonium, and other special nuclear materials for national defense, for other governmental uses, and for some civilian purposes. From August 1950 to March 31, 1989, SRS was operated for the Department of Energy (DOE) by E. I. du Pont de Nemours Co. On April 1, 1989 the Westinghouse Savannah River Company assumed responsibility as the prime contractor for the Savannah River Site.

  2. Seismic Hazard Characterization at the DOE Savannah River Site (SRS): Status report

    SciTech Connect

    Savy, J.B.

    1994-06-24

    The purpose of the Seismic Hazard Characterization project for the Savannah River Site (SRS-SHC) is to develop estimates of the seismic hazard for several locations within the SRS. Given the differences in the geology and geotechnical characteristics at each location, the estimates of the seismic hazard are to allow for the specific local conditions at each site. Characterization of seismic hazard is a critical factor for the design of new facilities as well as for the review and potential retrofit of existing facilities at SRS. The scope of the SRS seismic hazard characterization reported in this document is limited to the Probabilistic Seismic Hazard Analysis (PSHA). The goal of the project is to provide seismic hazard estimates based on a state-of-the-art method which is consistent with developments and findings of several ongoing studies which are deemed to bring improvements in the state of the seismic hazard analyses.

  3. Examining Reuse in LaSRS++-Based Projects

    NASA Technical Reports Server (NTRS)

    Madden, Michael M.

    2001-01-01

    NASA Langley Research Center (LaRC) developed the Langley Standard Real-Time Simulation in C++ (LaSRS++) to consolidate all software development for its simulation facilities under one common framework. A common framework promised a decrease in the total development effort for a new simulation by encouraging software reuse. To judge the success of LaSRS++ in this regard, reuse metrics were extracted from 11 aircraft models. Three methods that employ static analysis of the code were used to identify the reusable components. For the method that provides the best estimate, reuse levels fall between 66% and 95% indicating a high degree of reuse. Additional metrics provide insight into the extent of the foundation that LaSRS++ provides to new simulation projects. When creating variants of an aircraft, LaRC developers use object-oriented design to manage the aircraft as a reusable resource. Variants modify the aircraft for a research project or embody an alternate configuration of the aircraft. The variants inherit from the aircraft model. The variants use polymorphism to extend or redefine aircraft behaviors to meet the research requirements or to match the alternate configuration. Reuse level metrics were extracted from 10 variants. Reuse levels of aircraft by variants were 60% - 99%.

  4. Caviton collapse of SRS by SBS

    SciTech Connect

    Aldrich, C.; Bezzerides, B.; DuBois, D.F.; Rose, H.

    1984-01-01

    Particle simulations are performed to show that the ion waves resulting from Stimulated Brillouin Scatter (SBS) can trigger the collapse of the plasma waves excited by Stimulated Raman Scatter (SRS). We discuss the effect of this collapse mechanism on the hot-electron spectrum produced by the electrostatic waves. A fluid model for the coupling of the ion fluctuations to the plasma waves is formulated and we discuss the necessary condition on the SBS to induce collapse of the plasma waves produced by SRS.

  5. SRS browser: a visual interface to the sequence retrieval system

    NASA Astrophysics Data System (ADS)

    Mane, Ketan K.; Börner, Katy

    2006-01-01

    This paper presents a novel approach to the visual exploration and navigation of complex association networks of biological data sets, e.g., published papers, gene or protein information. The generic approach was implemented in the SRS Browser as an alternative visual interface to the highly used Sequence Retrieval System (SRS) [1]. SRS supports keyword-based search of about 400 biomedical databases. While the SRS presents search results as rank-ordered lists of matching entities, the SRS Browser displays entities and their relations for interactive exploration. A formal usability study was conducted to examine the SRS Browser interface's capabilities to support knowledge discovery and management.

  6. Groundwater Treatment at SRS: An Innovative Approach

    SciTech Connect

    Jorque, M.A.; Golshir, G.H.; Davis, B.

    1998-03-01

    The SRS is located in southwestern South Carolina, occupying an almost circular area of approximately 800 km2 within Aiken, Barnwell, and Allendale counties. The site lies approximately 36 km southeast of Augusta, Georgia, and is bounded by the Savannah River along its southwestern border. Prior to the establishment of the SRS in 1952, the area was largely a rural agricultural community. As part of the defense complex, the SRS produced special nuclear materials for the national defense.From 1955 until 1988, unlined earthen basins were used to dispose of wastewater from the SRS separations facilities located in the F and H areas. Approximately 300 million liters of wastewater was transported annually from the process area through underground piping to the basins. The wastewater was allowed to evaporate and to seep into the underlying formations. There were three basins in the F-Area covering a total of about 3 hectares; while the H-Area was served by four basins covering about 6 hectares. The seepage basins closure was started in 1989 and SCDHEC certified the closures as completed in 1991.Groundwater monitoring conducted in accordance with the provisions of the RCRA Permits determined that the underlying hydrogeologic units were contaminated by tritium, radioactive metals (primarily Cesium 137, Strontium 90, and Uranium 235), nitrate and heavy metals, some of which are defined as hazardous by RCRA. Under the terms and conditions of the RCRA Post- Closure Permits, it was necessary to remediate the contaminated groundwater plumes.

  7. Groundwater Treatment at SRS: An Innovative Approach

    SciTech Connect

    Jorque, M.A.; Golshir, G.H.; Davis, B.

    1998-03-01

    The SRS is located in southwestern South Carolina, occupying an almost circular area of approximately 800 km{sub 2} within Aiken, Barnwell, and Allendale counties. The site lies approximately 36 km southeast of Augusta, Georgia, and is bounded by the Savannah River along its southwestern border. Prior to the establishment of the SRS in 1952, the area was largely a rural agricultural community. As part of the defense complex, the SRS produced special nuclear materials for the national defense. From 1955 until 1988, unlined earthen basins were used to dispose of wastewater from the SRS separations facilities located in the F and H areas. Approximately 300 million liters of wastewater was transported annually from the process area through underground piping to the basins. The wastewater was allowed to evaporate and to seep into the underlying formations. There were three basins in the F-Area covering a total of about 3 hectares; while the H-Area was served by four basins covering about 6 hectares. The seepage basins closure was started in 1989 and SCDHEC certified the closures as completed in 1991. Groundwater monitoring conducted in accordance with the provisions of the RCRA Permits determined that the underlying hydrogeologic units were contaminated by tritium, radioactive metals (primarily Cesium{sup 137}, Strontium{sup 90}, and Uranium{sup 235}), nitrate and heavy metals, some of which are defined as hazardous by RCRA. Under the terms and conditions of the RCRA Post-Closure Permits, it was necessary to remediate the contaminated groundwater plumes.

  8. SRS Geology/Hydrogeology Environmental Information Document

    SciTech Connect

    Denham, M.E.

    1999-08-31

    The purpose of the Savannah River Site Geology and Hydrogeology Environmental Information Document (EID) is to provide geologic and hydrogeologic information to serve as a baseline to evaluate potential environmental impacts. This EID is based on a summary of knowledge accumulated from research conducted at the Savannah River Site (SRS) and surrounding areas.

  9. Brief Report: the Social Responsiveness Scale for Adults (SRS-A): initial results in a German cohort.

    PubMed

    Bölte, Sven

    2012-09-01

    The Social Responsiveness Scale (SRS) is a tool for quantitative autism assessment in children and adolescents. The SRS-A addresses social responsiveness in adulthood. Reliability and validity using the German adaptation of the SRS-A was examined in 20 adults with Autism Spectrum Disorder (ASD), 62 with other mental disorders (CLIN) and 163 typically developing (TD) participants. Cronbach's alpha ranged from .71 (TD) to .89 (ASD). A SRS-A total score of 67 had a sensitivity of .85, and a specificity of .83 for ASD versus CLIN/TD. Correlations with established autism scales (ADOS, AQ, SCQ) were moderate to high (r = .25-.83). Results provide adequate preliminary support for the application of the SRS-A. PMID:22183423

  10. TRANSIENT HEAT TRANSFER MODEL FOR SRS WASTE TANK OPERATIONS

    SciTech Connect

    Lee, S; Richard Dimenna, R

    2007-03-27

    A transient heat balance model was developed to assess the impact of a Submersible Mixer Pump (SMP) on waste temperature during the process of waste mixing and removal for the Type-I Savannah River Site (SRS) tanks. The model results will be mainly used to determine the SMP design impacts on the waste tank temperature during operations and to develop a specification for a new SMP design to replace existing long-shaft mixer pumps used during waste removal. The model will also be used to provide input to the operation planning. This planning will be used as input to pump run duration in order to maintain temperature requirements within the tank during SMP operation. The analysis model took a parametric approach. A series of the modeling analyses was performed to examine how submersible mixer pumps affect tank temperature during waste removal operation in the Type-I tank. The model domain included radioactive decay heat load, two SMP's, and one Submersible Transfer Pump (STP) as heat source terms. The present model was benchmarked against the test data obtained by the tank measurement to examine the quantitative thermal response of the tank and to establish the reference conditions of the operating variables under no SMP operation. The results showed that the model predictions agreed with the test data of the waste temperatures within about 10%. Transient modeling calculations for two potential scenarios of sludge mixing and removal operations have been made to estimate transient waste temperatures within a Type-I waste tank. When two 200-HP submersible mixers and 12 active cooling coils are continuously operated in 100-in tank level and 40 C initial temperature for 40 days since the initiation of mixing operation, waste temperature rises about 9 C in 48 hours at a maximum. Sensitivity studies for the key operating variables were performed. The sensitivity results showed that the chromate cooling coil system provided the primary cooling mechanism to remove process

  11. ENHANCED UNCERTAINTY ANALYSIS FOR SRS COMPOSITE ANALYSIS

    SciTech Connect

    Smith, F.; Phifer, M.

    2011-06-30

    sand and clay), (b) Dose Parameters (34 parameters), (c) Material Properties (20 parameters), (d) Surface Water Flows (6 parameters), and (e) Vadose and Aquifer Flow (4 parameters). Results provided an assessment of which group of parameters is most significant in the dose uncertainty. It was found that K{sub d} and the vadose/aquifer flow parameters, both of which impact transport timing, had the greatest impact on dose uncertainty. Dose parameters had an intermediate level of impact while material properties and surface water flows had little impact on dose uncertainty. Results of the importance analysis are discussed further in Section 7 of this report. The objectives of this work were to address comments received during the CA review on the uncertainty analysis and to demonstrate an improved methodology for CA uncertainty calculations as part of CA maintenance. This report partially addresses the LFRG Review Team issue of producing an enhanced CA sensitivity and uncertainty analysis. This is described in Table 1-1 which provides specific responses to pertinent CA maintenance items extracted from Section 11 of the SRS CA (2009). As noted above, the original uncertainty analysis looked at each POA separately and only included the effects from at most five sources giving the highest peak doses at each POA. Only 17 of the 152 CA sources were used in the original uncertainty analysis and the simulation time was reduced from 10,000 to 2,000 years. A major constraint on the original uncertainty analysis was the limitation of only being able to use at most four distributed processes. This work expanded the analysis to 10,000 years using 39 of the CA sources, included cumulative dose effects at downstream POAs, with more realizations (1,000) and finer time steps. This was accomplished by using the GoldSim DP-Plus module and the 36 processors available on a new windows cluster. The last part of the work looked at the contribution to overall uncertainty from the main

  12. Groundwater Monitoring Optimization of Post Closure Waste Sites at SRS - 13184

    SciTech Connect

    Ross, Jeff; O'Quinn, Sadika; Adams, Karen; Prater, Phil

    2013-07-01

    Groundwater monitoring at the Savannah River Site (SRS) is required at dozens of waste sites and includes sampling at over 1,000 monitoring wells. The expected longevity of groundwater contamination and associated groundwater monitoring and reporting constitutes a significant long-term cost that represents an increasing proportion of the environmental management budget as surface waste units are closed. Therefore, a comprehensive evaluation of the monitoring program for eighteen regulated waste units was conducted to identify areas where monitoring could be optimized. The units evaluated varied considerably in the scope of monitoring; ranging from two wells to hundreds of wells. In order to systematically evaluate such disparate monitoring networks, SRS developed a decision-logic analysis using flow sheets to address potential areas of optimization. Five areas were identified for evaluation, including: (1) Comparison of current monitoring to regulatory requirements, (2) Spatial distribution, (3) Temporal sampling, (4) Analyte requirements, and (5) Reporting frequency and content. Optimization recommendations were made for fifteen of the eighteen groundwater units. The spatial evaluation resulted in recommendations to suspend sampling in 79 wells and add sampling at 16 wells. The temporal evaluation resulted in recommendations to reduce the number of well visits per year by 504. Analyte reductions were recommended at three groundwater units, with increases at three other units. Reporting frequency reductions were recommended for five units. Approximately $700,000 (direct dollars) of potential annualized cost savings were identified for these groundwater units, provided all recommendations are approved. The largest area of savings was associated with reducing the reporting frequency. The optimization approach has been presented to the EPA and South Carolina Department of Environmental Control (SCHDEC), with unit-specific recommendations approved for all five units

  13. 29 CFR 1926.651 - Specific excavation requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) or more in depth so as to require no more than 25 feet (7.62 m) of lateral travel for employees. (d... by lifting or digging equipment. Employees shall be required to stand away from any vehicle being... the cabs of vehicles being loaded or unloaded when the vehicles are equipped, in accordance...

  14. Repackaging SRS Black Box TRU Waste

    SciTech Connect

    Swale, D. J.; Stone, K.A.; Milner, T. N.

    2006-01-09

    Historically, large items of TRU Waste, which were too large to be packaged in drums for disposal have been packaged in various sizes of custom made plywood boxes at the Savannah River Site (SRS), for many years. These boxes were subsequently packaged into large steel ''Black Boxes'' for storage at SRS, pending availability of Characterization and Certification capability, to facilitate disposal of larger items of TRU Waste. There are approximately 107 Black Boxes in inventory at SRS, each measuring some 18' x 12' x 7', and weighing up to 45,000 lbs. These Black Boxes have been stored since the early 1980s. The project to repackage this waste into Standard Large Boxes (SLBs), Standard Waste Boxes (SWB) and Ten Drum Overpacks (TDOP), for subsequent characterization and WIPP disposal, commenced in FY04. To date, 10 Black Boxes have been repackaged, resulting in 40 SLB-2's, and 37 B25 overpack boxes, these B25's will be overpacked in SLB-2's prior to shipping to WIPP. This paper will describe experience to date from this project.

  15. 49 CFR 179.101-1 - Individual specification requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... than 1/2 inch. 4 Tank cars not equipped with a thermal protection or an insulation system used for the... are as follows: DOT specification Insulation Bursting pressure (psig) Minimum plate thickness...

  16. 46 CFR 63.20-1 - Specific control system requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... emergency safety trip control operation, the air flow to the boiler must not automatically increase. For... addition to the requirements found in ASME CSD-1 (incorporated by reference; see 46 CFR 63.05-1),...

  17. SWEPP assay system version 2.0 software requirements specification

    SciTech Connect

    Matthews, S.D.; East, L.V.; Marwil, E.S.; Ferguson, J.J.

    1996-06-01

    The INEL Stored Waste Examination Pilot Plant (SWEPP) operations staff use nondestructive analysis methods to characterize the radiological contents of contact-handled radioactive waste containers. Containers of waste from Rocky Flats Environmental Technology Site and other DOE sites are currently stored at SWEPP. Before these containers can be shipped to WIPP, SWEPP must verify compliance with storage, shipping, and disposal requirements. One part of the SWEPP program measures neutron emissions from the containers and estimates the mass of Pu and other transuranic isotopes present. The code NEUT2 was originally used to perform data acquisition and reduction; the SWEPP Assay System (SAS) code replaced NEUT2 in early 1994. This document specifies the requirements for the SAS software as installed at INEL and was written to comply with RWMC (INEL Radioactive Waste Management Complex) quality requirements.

  18. 49 CFR 178.35 - General requirements for specification cylinders.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... specification by— (i) Making a chemical analysis of each heat of material; (ii) Obtaining a certified chemical analysis from the material manufacturer for each heat of material (a ladle analysis is acceptable); or (iii) If an analysis is not provided for each heat of material by the material manufacturer, by making...

  19. 49 CFR 179.101-1 - Individual specification requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS SPECIFICATIONS... tensile strength is used, the thickness of plates shall be not less than 5/8 inch, and when steel of 81,000 p.s.i. minimum tensile strength is used, the minimum thickness of plate shall be not less than...

  20. 40 CFR 35.3550 - Specific capitalization grant agreement requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... subpart, the general grant regulations at 40 CFR part 31, and specific conditions of the grant. A State... public water systems receiving assistance will use accounting, audit, and fiscal procedures conforming to... Board or, in the case of privately-owned systems, the Financial Accounting Standards Board....

  1. 40 CFR 35.3550 - Specific capitalization grant agreement requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... subpart, the general grant regulations at 40 CFR part 31, and specific conditions of the grant. A State... public water systems receiving assistance will use accounting, audit, and fiscal procedures conforming to... Board or, in the case of privately-owned systems, the Financial Accounting Standards Board....

  2. 40 CFR 35.3550 - Specific capitalization grant agreement requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... subpart, the general grant regulations at 40 CFR part 31, and specific conditions of the grant. A State... public water systems receiving assistance will use accounting, audit, and fiscal procedures conforming to... Board or, in the case of privately-owned systems, the Financial Accounting Standards Board....

  3. 49 CFR 178.35 - General requirements for specification cylinders.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... specification by— (i) Making a chemical analysis of each heat of material; (ii) Obtaining a certified chemical analysis from the material manufacturer for each heat of material (a ladle analysis is acceptable); or (iii) If an analysis is not provided for each heat of material by the material manufacturer, by making...

  4. 7 CFR 1753.6 - Standards, specifications, and general requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... construction financed with loan funds must meet the standards and specifications established by RUS. 7 CFR part... funds to finance nonstandard construction materials or equipment only if approved by RUS in writing prior to purchase or commencement of construction. (c) Only new materials and equipment may be...

  5. 46 CFR 63.20-1 - Specific control system requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... this condition, postpurge must be accomplished manually. (b) Combustion control system. A low fire interlock must ensure low fire start when variable firing rates are used. (c) Water level controls and low... addition to the requirements found in ASME CSD-1 (incorporated by reference; see 46 CFR 63.05-1),...

  6. 46 CFR 63.20-1 - Specific control system requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... emergency safety trip control operation, the air flow to the boiler must not automatically increase. For....20-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC... addition to the requirements found in ASME CSD-1 (incorporated by reference; see 46 CFR 63.05-1),...

  7. 46 CFR 63.20-1 - Specific control system requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... emergency safety trip control operation, the air flow to the boiler must not automatically increase. For....20-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC... addition to the requirements found in ASME CSD-1 (incorporated by reference; see 46 CFR 63.05-1),...

  8. 40 CFR 86.1406 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) Emission Regulations for New Gasoline-Fueled Otto-Cycle Light-Duty Vehicles and New Gasoline-Fueled Otto-Cycle Light-Duty Trucks; Certification Short Test Procedures § 86.1406 Equipment required and..., the dynamometer must be adjusted to the power absorption unit and inertia weight settings as...

  9. 40 CFR 86.1406 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...) Emission Regulations for New Gasoline-Fueled Otto-Cycle Light-Duty Vehicles and New Gasoline-Fueled Otto-Cycle Light-Duty Trucks; Certification Short Test Procedures § 86.1406 Equipment required and..., the dynamometer must be adjusted to the power absorption unit and inertia weight settings as...

  10. 40 CFR 86.1406 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) Emission Regulations for New Gasoline-Fueled Otto-Cycle Light-Duty Vehicles and New Gasoline-Fueled Otto-Cycle Light-Duty Trucks; Certification Short Test Procedures § 86.1406 Equipment required and..., the dynamometer must be adjusted to the power absorption unit and inertia weight settings as...

  11. 29 CFR 1926.651 - Specific excavation requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... excavation. (g) Hazardous atmospheres—(1) Testing and controls. In addition to the requirements set forth in subparts D and E of this part (29 CFR 1926.50-1926.107) to prevent exposure to harmful levels of... apply: (i) Where oxygen deficiency (atmospheres containing less than 19.5 percent oxygen) or a...

  12. 40 CFR 86.1406 - Equipment required and specifications; overview.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) Emission Regulations for New Gasoline-Fueled Otto-Cycle Light-Duty Vehicles and New Gasoline-Fueled Otto-Cycle Light-Duty Trucks; Certification Short Test Procedures § 86.1406 Equipment required and... adjusted to the lowest available inertia weight setting and must meet the load speed...

  13. 47 CFR 76.75 - Specific EEO program requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... opportunity notices, where appropriate; (2) Placing a notice in bold type on the employment application... maintenance of web sites that provide counseling on the process of searching for multichannel video... vacancies; (4) Documentation necessary to demonstrate performance of the initiatives required by...

  14. 47 CFR 76.75 - Specific EEO program requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... opportunity notices, where appropriate; (2) Placing a notice in bold type on the employment application... maintenance of web sites that provide counseling on the process of searching for multichannel video... vacancies; (4) Documentation necessary to demonstrate performance of the initiatives required by...

  15. 49 CFR 173.62 - Specific packaging requirements for explosives.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... explosive substance or article if an equivalent level of safety is shown to be maintained subject to the... packagings are not required if drums are used as the outer packaging. 4. At least one of the packagings must... be so constructed that the risk of explosion, by reason of increased internal pressure from......

  16. JET MIXING ANALYSIS FOR SRS HIGH-LEVEL WASTE RECOVERY

    SciTech Connect

    Lee, S.

    2011-07-05

    The process of recovering the waste in storage tanks at the Savannah River Site (SRS) typically requires mixing the contents of the tank to ensure uniformity of the discharge stream. Mixing is accomplished with one to four slurry pumps located within the tank liquid. The slurry pump may be fixed in position or they may rotate depending on the specific mixing requirements. The high-level waste in Tank 48 contains insoluble solids in the form of potassium tetraphenyl borate compounds (KTPB), monosodium titanate (MST), and sludge. Tank 48 is equipped with 4 slurry pumps, which are intended to suspend the insoluble solids prior to transfer of the waste to the Fluidized Bed Steam Reformer (FBSR) process. The FBSR process is being designed for a normal feed of 3.05 wt% insoluble solids. A chemical characterization study has shown the insoluble solids concentration is approximately 3.05 wt% when well-mixed. The project is requesting a Computational Fluid Dynamics (CFD) mixing study from SRNL to determine the solids behavior with 2, 3, and 4 slurry pumps in operation and an estimate of the insoluble solids concentration at the suction of the transfer pump to the FBSR process. The impact of cooling coils is not considered in the current work. The work consists of two principal objectives by taking a CFD approach: (1) To estimate insoluble solids concentration transferred from Tank 48 to the Waste Feed Tank in the FBSR process and (2) To assess the impact of different combinations of four slurry pumps on insoluble solids suspension and mixing in Tank 48. For this work, several different combinations of a maximum of four pumps are considered to determine the resulting flow patterns and local flow velocities which are thought to be associated with sludge particle mixing. Two different elevations of pump nozzles are used for an assessment of the flow patterns on the tank mixing. Pump design and operating parameters used for the analysis are summarized in Table 1. The baseline

  17. Review of seismicity and ground motion studies related to development of seismic design at SRS

    SciTech Connect

    Stephenson, D.E.; Acree, J.R.

    1992-08-01

    The NRC response spectra developed in Reg. Guide 1.60 is being used in the studies related to restarting of the existing Savannah River Site (SRS) reactors. Because it envelopes all the other site specific spectra which have been developed for SRS, it provides significant conservatism in the design and analysis of the reactor systems for ground motions of this value or with these probability levels. This spectral shape is also the shape used for the design of the recently licensed Vogtle Nuclear Station, located south of the Savannah River from the SRS. This report provides a summary of the data base used to develop the design basis earthquake. This includes the seismicity, rates of occurrence, magnitudes, and attenuation relationships. A summary is provided for the studies performed and methodologies used to establish the design basis earthquake for SRS. The ground motion response spectra developed from the various studies are also summarized. The seismic hazard and PGA`s developed for other critical facilities in the region are discussed, and the SRS seismic instrumentation is presented. The programs for resolving outstanding issues are discussed and conclusions are presented.

  18. Summary of the SRS Severe Accident Analysis Program, 1987--1992

    SciTech Connect

    Long, T.A.; Hyder, M.L.; Britt, T.E.; Allison, D.K.; Chow, S.; Graves, R.D.; DeWald, A.B. Jr.; Monson, P.R. Jr.; Wooten, L.A.

    1992-11-01

    The Severe Accident Analysis Program (SAAP) is a program of experimental and analytical studies aimed at characterizing severe accidents that might occur in the Savannah River Site Production Reactors. The goals of the Severe Accident Analysis Program are: To develop an understanding of severe accidents in SRS reactors that is adequate to support safety documentation for these reactors, including the Safety Analysis Report (SAR), the Probabilistic Risk Assessment (PRA), and other studies evaluating the safety of reactor operation; To provide tools and bases for the evaluation of existing or proposed safety related equipment in the SRS reactors; To provide bases for the development of accident management procedures for the SRS reactors; To develop and maintain on the site a sufficient body of knowledge, including documents, computer codes, and cognizant engineers and scientists, that can be used to authoritatively resolve questions or issues related to reactor accidents. The Severe Accident Analysis Program was instituted in 1987 and has already produced a substantial amount of information, and specialized calculational tools. Products of the Severe Accident Analysis Program (listed in Section 9 of this report) have been used in the development of the Safety Analysis Report (SAR) and the Probabilistic Risk Assessment (PRA), and in the development of technical specifications for the SRS reactors. A staff of about seven people is currently involved directly in the program and in providing input on severe accidents to other SRS activities.

  19. Dismantlement and decontamination of a plutonium-238 facility at SRS

    SciTech Connect

    Smith, R.H. Jr.; Hootman, H.E.

    1994-01-01

    There has been very little, documented decontamination and decommissioning (D&D) experience on which to project cleanup costs and schedules for plutonium facilities at SRS and other DOE sites. A portion of the HB-Line, a plutonium-238 processing facility at SRS, has been undergoing D&D intermittently since 1984. Although this cleanup effort was not originally intended to quantify results, some key data have been project has demonstrated effective methods of accumulated, and the performing D&D work, and has demonstrated cleanup equipment and techniques under conditions of high contamination. Plutonium facilities where D&D is already underway provide an opportunity for` timely field testing of characterization, size reduction, and decontamination techniques. Some data are presented here; however, more specific tests and data may be obtained during the remainder of this project. This project has been recommended as a candidate test facility for a DOE planned ``Integrated D&D Demonstration`` managed by EM-50 to develop and demonstrate technology for D&D and surplus facilities deactivation. Both the remainder of this project and the Integrated D&D Demonstration Program can benefit from a joint effort, and the, overall costs should be reduced.

  20. Thermodynamic Modeling of the SRS Evaporators: Part II. The 3H System

    SciTech Connect

    Jantzen, C.M.

    2001-10-02

    Accumulations of two solid phases have formed scale deposits in the Savannah River Site 2H Evaporator system since late 1996. The aluminosilicate scale deposits caused the evaporator pot to become inoperable in October 1999. Accumulations of the diuranate phase have caused criticality concerns in the SRS 2H Evaporator. In order to ensure that similar deposits are not and will not form in the SRS 3H Evaporator, thermodynamically derived activity diagrams specific to the feeds processed from Tanks 30 and 32 are evaluated in this report.

  1. Pilot study risk assessment for selected problems at the Savannah River Site (SRS)

    SciTech Connect

    Hamilton, L.D.; Holtzman, S.; Meinhold, A.; Morris, S.C.; Pardi, R.; Sun, C. ); Daniels, J.I.; Layton, D.; McKone, T.E.; Straume, T.; Anspaugh, L. )

    1993-03-01

    An assessment of the health risks was made for releases of tritium and [sup 137]Cs from the Savannah River Site (SRS) at water-receptor locations downriver. Although reactor operations were shut down at the SRS in 1989, liquid wastes continue to be released to the Savannah River either by direct discharges into onsite surface waters or by groundwater transport into surface waters from waste facilities. Existing state mandates will cause the liquid waste streams from future operations to go directly into surface waters. Two drinking water processing plants take water from the river approximately 129 km downriver from the SRS. Potential incremental risks of cancer fatality to individuals and each population were analyzed for either no further reactor operations or resumption of operation of one specific reactor.

  2. Pilot study risk assessment for selected problems at the Savannah River Site (SRS)

    SciTech Connect

    Hamilton, L.D.; Holtzman, S.; Meinhold, A.; Morris, S.C.; Pardi, R.; Sun, C.; Daniels, J.I.; Layton, D.; McKone, T.E.; Straume, T.; Anspaugh, L.

    1993-03-01

    An assessment of the health risks was made for releases of tritium and {sup 137}Cs from the Savannah River Site (SRS) at water-receptor locations downriver. Although reactor operations were shut down at the SRS in 1989, liquid wastes continue to be released to the Savannah River either by direct discharges into onsite surface waters or by groundwater transport into surface waters from waste facilities. Existing state mandates will cause the liquid waste streams from future operations to go directly into surface waters. Two drinking water processing plants take water from the river approximately 129 km downriver from the SRS. Potential incremental risks of cancer fatality to individuals and each population were analyzed for either no further reactor operations or resumption of operation of one specific reactor.

  3. Input to the PRAST computer code used in the SRS probabilistic risk assessment

    SciTech Connect

    Kearnaghan, D.P.

    1992-10-15

    The PRAST (Production Reactor Algorithm for Source Terms) computer code was developed by Westinghouse Savannah River Company and Science Application International Corporation for the quantification of source terms for the SRS Savannah River Site (SRS) Reactor Probabilistic Risk Assessment. PRAST requires as input a set of release fractions, decontamination factors, transfer fractions and source term characteristics that accurately reflect the conditions that are evaluated by PRAST. This document links the analyses which form the basis for the PRAST input parameters. In addition, it gives the distribution of the input parameters that are uncertain and considered to be important to the evaluation of the source terms to the environment.

  4. End User Acceptance - Requirements or Specifications, Certification, Testing

    NASA Technical Reports Server (NTRS)

    Jeevarajan, Judith

    2013-01-01

    NASA follows top level safety requirement of two-failure tolerance (t hree levels of controls or design for minimum risk) to all catastroph ic hazards in the design of safe li-ion batteries for space use. ? R igorous development testing at appropriate levels to credible offnominal conditions and review of test data. ? Implement robust design con trols based on test results and test again to confirm safety at the a ppropriate levels. ? Stringent testing of all (100%) flight batteries (from button cells to large batteries).

  5. Software requirements specification document for the AREST code development

    SciTech Connect

    Engel, D.W.; McGrail, B.P.; Whitney, P.D.; Gray, W.J.; Williford, R.E.; White, M.D.; Eslinger, P.W.; Altenhofen, M.K.

    1993-11-01

    The Analysis of the Repository Source Term (AREST) computer code was selected in 1992 by the U.S. Department of Energy. The AREST code will be used to analyze the performance of an underground high level nuclear waste repository. The AREST code is being modified by the Pacific Northwest Laboratory (PNL) in order to evaluate the engineered barrier and waste package designs, model regulatory compliance, analyze sensitivities, and support total systems performance assessment modeling. The current version of the AREST code was developed to be a very useful tool for analyzing model uncertainties and sensitivities to input parameters. The code has also been used successfully in supplying source-terms that were used in a total systems performance assessment. The current version, however, has been found to be inadequate for the comparison and selection of a design for the waste package. This is due to the assumptions and simplifications made in the selection of the process and system models. Thus, the new version of the AREST code will be designed to focus on the details of the individual processes and implementation of more realistic models. This document describes the requirements of the new models that will be implemented. Included in this document is a section describing the near-field environmental conditions for this waste package modeling, description of the new process models that will be implemented, and a description of the computer requirements for the new version of the AREST code.

  6. PAMS photo image retrieval prototype system requirements specification

    SciTech Connect

    Conner, M.L.

    1996-04-30

    This project is part of the Photo Audiovisual Management System (PAMS). The project was initially identified in 1989 and has since been has been worked on under various names such as Image Retrieval and Viewing System, Photo Image Retrieval Subsystem and Image Processing and Compression System. This document builds upon the information collected and the analysis performed in the earlier phases of this project. The PAMS Photo Imaging subsystem will provide the means of capturing low resolution digital images from Photography`s negative files and associating the digital images with a record in the PAMS photo database. The digital images and key photo identification information will be accessible to HAN users to assist in locating and identifying specific photographs. After identifying desired photographs, users may request photo prints or high resolution digital images directly from Photography. The digital images captured by this project are for identification purposes only and are not intended to be of sufficient quality for subsequent use.

  7. HSCT4.0 Application: Software Requirements Specification

    NASA Technical Reports Server (NTRS)

    Salas, A. O.; Walsh, J. L.; Mason, B. H.; Weston, R. P.; Townsend, J. C.; Samareh, J. A.; Green, L. L.

    2001-01-01

    The software requirements for the High Performance Computing and Communication Program High Speed Civil Transport application project, referred to as HSCT4.0, are described. The objective of the HSCT4.0 application project is to demonstrate the application of high-performance computing techniques to the problem of multidisciplinary design optimization of a supersonic transport configuration, using high-fidelity analysis simulations. Descriptions of the various functions (and the relationships among them) that make up the multidisciplinary application as well as the constraints on the software design arc provided. This document serves to establish an agreement between the suppliers and the customer as to what the HSCT4.0 application should do and provides to the software developers the information necessary to design and implement the system.

  8. Specification of advanced safety modeling requirements (Rev. 0).

    SciTech Connect

    Fanning, T. H.; Tautges, T. J.

    2008-06-30

    The U.S. Department of Energy's Global Nuclear Energy Partnership has lead to renewed interest in liquid-metal-cooled fast reactors for the purpose of closing the nuclear fuel cycle and making more efficient use of future repository capacity. However, the U.S. has not designed or constructed a fast reactor in nearly 30 years. Accurate, high-fidelity, whole-plant dynamics safety simulations will play a crucial role by providing confidence that component and system designs will satisfy established design limits and safety margins under a wide variety of operational, design basis, and beyond design basis transient conditions. Current modeling capabilities for fast reactor safety analyses have resulted from several hundred person-years of code development effort supported by experimental validation. The broad spectrum of mechanistic and phenomenological models that have been developed represent an enormous amount of institutional knowledge that needs to be maintained. Complicating this, the existing code architectures for safety modeling evolved from programming practices of the 1970s. This has lead to monolithic applications with interdependent data models which require significant knowledge of the complexities of the entire code in order for each component to be maintained. In order to develop an advanced fast reactor safety modeling capability, the limitations of the existing code architecture must be overcome while preserving the capabilities that already exist. To accomplish this, a set of advanced safety modeling requirements is defined, based on modern programming practices, that focuses on modular development within a flexible coupling framework. An approach for integrating the existing capabilities of the SAS4A/SASSYS-1 fast reactor safety analysis code into the SHARP framework is provided in order to preserve existing capabilities while providing a smooth transition to advanced modeling capabilities. In doing this, the advanced fast reactor safety models will

  9. Development of SRS.php, a Simple Object Access Protocol-based library for data acquisition from integrated biological databases.

    PubMed

    Barbosa-Silva, A; Pafilis, E; Ortega, J M; Schneider, R

    2007-12-11

    Data integration has become an important task for biological database providers. The current model for data exchange among different sources simplifies the manner that distinct information is accessed by users. The evolution of data representation from HTML to XML enabled programs, instead of humans, to interact with biological databases. We present here SRS.php, a PHP library that can interact with the data integration Sequence Retrieval System (SRS). The library has been written using SOAP definitions, and permits the programmatic communication through webservices with the SRS. The interactions are possible by invoking the methods described in WSDL by exchanging XML messages. The current functions available in the library have been built to access specific data stored in any of the 90 different databases (such as UNIPROT, KEGG and GO) using the same query syntax format. The inclusion of the described functions in the source of scripts written in PHP enables them as webservice clients to the SRS server. The functions permit one to query the whole content of any SRS database, to list specific records in these databases, to get specific fields from the records, and to link any record among any pair of linked databases. The case study presented exemplifies the library usage to retrieve information regarding registries of a Plant Defense Mechanisms database. The Plant Defense Mechanisms database is currently being developed, and the proposal of SRS.php library usage is to enable the data acquisition for the further warehousing tasks related to its setup and maintenance.

  10. Robust Entrainment of Circadian Oscillators Requires Specific Phase Response Curves

    PubMed Central

    Pfeuty, Benjamin; Thommen, Quentin; Lefranc, Marc

    2011-01-01

    The circadian clocks keeping time in many living organisms rely on self-sustained biochemical oscillations entrained by external cues, such as light, to the 24-h cycle induced by Earth's rotation. However, environmental cues are unreliable due to the variability of habitats, weather conditions, or cue-sensing mechanisms among individuals. A tempting hypothesis is that circadian clocks have evolved so as to be robust to fluctuations in the signal that entrains them. To support this hypothesis, we analyze the synchronization behavior of weakly and periodically forced oscillators in terms of their phase response curve (PRC), which measures phase changes induced by a perturbation applied at different times of the cycle. We establish a general relationship between the robustness of key entrainment properties, such as stability and oscillator phase, on the one hand, and the shape of the PRC as characterized by a specific curvature or the existence of a dead zone, on the other hand. The criteria obtained are applied to computational models of circadian clocks and account for the disparate robustness properties of various forcing schemes. Finally, the analysis of PRCs measured experimentally in several organisms strongly suggests a case of convergent evolution toward an optimal strategy for maintaining a clock that is accurate and robust to environmental fluctuations. PMID:21641300

  11. Solar powered biohydrogen production requires specific localization of the hydrogenase

    DOE PAGES

    Burroughs, Nigel J.; Boehm, Marko; Eckert, Carrie; Mastroianni, Giulia; Spence, Edward M.; Yu, Jianfeng; Nixon, Peter J.; Appel, Jens; Mullineaux, Conrad W.; Bryan, Samantha J.

    2014-09-04

    Cyanobacteria contain a bidirectional [NiFe] hydrogenase which transiently produces hydrogen upon exposure of anoxic cells to light, potentially acting as a “valve” releasing excess electrons from the electron transport chain. However, its interaction with the photosynthetic electron transport chain remains unclear. By GFP-tagging the HoxF diaphorase subunit we show that the hydrogenase is thylakoid associated, comprising a population dispersed uniformly through the thylakoids and a subpopulation localized to discrete puncta in the distal thylakoid. Thylakoid localisation of both the HoxH and HoxY hydrogenase subunits is confirmed by immunogold electron microscopy. The diaphorase HoxE subunit is essential for recruitment to themore » dispersed thylakoid population, potentially anchoring the hydrogenase to the membrane, but aggregation to puncta occurs through a distinct HoxE-independent mechanism. Membrane association does not require NDH-1. Localization is dynamic on a scale of minutes, with anoxia and high light inducing a significant redistribution between these populations in favour of puncta. Lastly, since HoxE is essential for access to its electron donor, electron supply to the hydrogenase depends on a physiologically controlled localization, potentially offering a new avenue to enhance photosynthetic hydrogen production by exploiting localization/aggregation signals.« less

  12. Solar powered biohydrogen production requires specific localization of the hydrogenase

    SciTech Connect

    Burroughs, Nigel J.; Boehm, Marko; Eckert, Carrie; Mastroianni, Giulia; Spence, Edward M.; Yu, Jianfeng; Nixon, Peter J.; Appel, Jens; Mullineaux, Conrad W.; Bryan, Samantha J.

    2014-09-04

    Cyanobacteria contain a bidirectional [NiFe] hydrogenase which transiently produces hydrogen upon exposure of anoxic cells to light, potentially acting as a “valve” releasing excess electrons from the electron transport chain. However, its interaction with the photosynthetic electron transport chain remains unclear. By GFP-tagging the HoxF diaphorase subunit we show that the hydrogenase is thylakoid associated, comprising a population dispersed uniformly through the thylakoids and a subpopulation localized to discrete puncta in the distal thylakoid. Thylakoid localisation of both the HoxH and HoxY hydrogenase subunits is confirmed by immunogold electron microscopy. The diaphorase HoxE subunit is essential for recruitment to the dispersed thylakoid population, potentially anchoring the hydrogenase to the membrane, but aggregation to puncta occurs through a distinct HoxE-independent mechanism. Membrane association does not require NDH-1. Localization is dynamic on a scale of minutes, with anoxia and high light inducing a significant redistribution between these populations in favour of puncta. Lastly, since HoxE is essential for access to its electron donor, electron supply to the hydrogenase depends on a physiologically controlled localization, potentially offering a new avenue to enhance photosynthetic hydrogen production by exploiting localization/aggregation signals.

  13. SRS baseline hydrogeologic investigation: Summary report

    SciTech Connect

    Bledsoe, H.W.; Aadland, R.K. ); Sargent, K.A. . Dept. of Geology)

    1990-11-01

    Work on the Savannah River Site (SRS) Baseline Hydrogeologic Investigation began in 1983 when it was determined that the knowledge of the plant hydrogeologic systems needed to be expanded and improved in response to changing stratigraphic and hydrostratigraphic terminology and increased involvement by regulatory agencies (Bledsoe, 1984). Additionally, site-wide data were needed to determine flow paths, gradients, and velocities associated with the different aquifers underlying the plant site. The program was divided into three phases in order to allow the results of one phase to be evaluated and necessary changes and improvements incorporated into the following phases. This report summarizes the results of all three phases and includes modified graphic logs, lithologic descriptions of the different geologic formations, profiles of each cluster site, hydrostratigraphic cross sections, hydrographs of selected wells within each cluster for the first full year of uninterrupted water level measurements, potentiometric maps developed from data collected from all clusters, completion diagrams for each well, and a summary of laboratory tests. Additionally, the proposed new classification of hydrostratigraphic units at SRS (Aadland and Bledsoe, 1990) has been incorporated.

  14. SRS stainless steel beneficial reuse program

    SciTech Connect

    Boettinger, W.L.

    1997-02-01

    The US Department of Energy`s (DOE) Savannah River Site (SRS) has thousands of tons of stainless steel radioactive scrap metal (RSNI). Much of the metal is volumetrically contaminated. There is no {open_quotes}de minimis{close_quotes} free release level for volumetric material, and therefore no way to recycle the metal into the normal commercial market. If declared waste, the metal would qualify as low level radioactive waste (LLW) and ultimately be dispositioned through shallow land buried at a cost of millions of dollars. The metal however could be recycled in a {open_quotes}controlled release{close_quote} manner, in the form of containers to hold other types of radioactive waste. This form of recycle is generally referred to as {open_quotes}Beneficial Reuse{close_quotes}. Beneficial reuse reduces the amount of disposal space needed and reduces the need for virgin containers which would themselves become contaminated. Stainless steel is particularly suited for long term storage because of its resistance to corrosion. To assess the practicality of stainless steel RSM recycle the SRS Benficial Reuse Program began a demonstration in 1994, funded by the DOE Office of Science and Technology. This paper discusses the experiences gained in this program.

  15. The O-Methyltransferase SrsB Catalyzes the Decarboxylative Methylation of Alkylresorcylic Acid during Phenolic Lipid Biosynthesis by Streptomyces griseus

    PubMed Central

    Nakano, Chiaki; Funa, Nobutaka; Horinouchi, Sueharu

    2012-01-01

    Streptomyces griseus contains the srs operon, which is required for phenolic lipid biosynthesis. The operon consists of srsA, srsB, and srsC, which encode a type III polyketide synthase, an O-methyltransferase, and a flavoprotein hydroxylase, respectively. We previously reported that the recombinant SrsA protein synthesized 3-(13′-methyltetradecyl)-4-methylresorcinol, using iso-C16 fatty acyl-coenzyme A (CoA) as a starter substrate and malonyl-CoA and methylmalonyl-CoA as extender substrates. An in vitro SrsA reaction using [13C3]malonyl-CoA confirmed that the order of extender substrate condensation was methylmalonyl-CoA, followed by two extensions with malonyl-CoA. Furthermore, SrsA was revealed to produce an alkylresorcylic acid as its direct product rather than an alkylresorcinol. The functional SrsB protein was produced in the membrane fraction in Streptomyces lividans and used for the in vitro SrsB reaction. When the SrsA reaction was coupled, SrsB produced alkylresorcinol methyl ether in the presence of S-adenosyl-l-methionine (SAM). SrsB was incapable of catalyzing the O-methylation of alkylresorcinol, indicating that alkylresorcylic acid was the substrate of SrsB and that SrsB catalyzed the conversion of alkylresorcylic acid to alkylresorcinol methyl ether, namely, by both the O-methylation of the hydroxyl group (C-6) and the decarboxylation of the neighboring carboxyl group (C-1). O-methylated alkylresorcylic acid was not detected in the in vitro SrsAB reaction, although it was presumably stable, indicating that O-methylation did not precede decarboxylation. We therefore postulated that O-methylation was coupled with decarboxylation and proposed that SrsB catalyzed the feasible SAM-dependent decarboxylative methylation of alkylresorcylic acid. To the best of our knowledge, this is the first report of a methyltransferase that catalyzes decarboxylative methylation. PMID:22247507

  16. Assessing autistic traits in a Taiwan preschool population: cross-cultural validation of the Social Responsiveness Scale (SRS).

    PubMed

    Wang, Jessica; Lee, Li-Ching; Chen, Ying-Sheue; Hsu, Ju-Wei

    2012-11-01

    The cross-cultural validity of the Mandarin-adaptation of the social responsiveness scale (SRS) was examined in a sample of N = 307 participants in Taiwan, 140 typically developing and 167 with clinically-diagnosed developmental disorders. This scale is an autism assessment tool that provides a quantitative rather than categorical measure of social impairment in the general population. SRS total and subscale scores distinguished significantly between autism spectrum disorder and other developmental disorders (p < 0.01). Total SRS scores and sensitivity and specificity of the scale for diagnosing developmental disorders in the Taiwan study were similar to those observed in Western studies. These findings support the cross-cultural validity of the SRS scale for detecting autistic traits and for distinguishing between autism and other neuropsychiatric conditions. PMID:22407579

  17. Development of a Rotary Microfilter for SRS HLW Applications

    SciTech Connect

    MICHAEL, POIRIER

    2004-11-24

    The processing rate of Savannah River Site high level waste decontamination processes are limited by the flow rate of the solid-liquid separation. The baseline process, using a 0.1 micron cross flow filter, produces 0.02 gpm/ft2 of filtrate under expected operating conditions. Savannah River National Laboratory personnel identified the rotary microfilter as a technology that could significantly increase filter flux, with throughput improvements of as much as 10X for that specific operation. With funding from the Department of Energy Office of Cleanup Technologies, SRNL personnel are evaluating and developing the rotary microfilter for radioactive service at SRS. This work includes pilot-scale and actual waste testing to evaluate system reliability, the impact of radiation on system components, the filter flux for a variety of waste streams, and relative performance for alternative filter media.

  18. 40 CFR 166.32 - Reporting and recordkeeping requirements for specific, quarantine, and public health exemptions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... requirements for specific, quarantine, and public health exemptions. 166.32 Section 166.32 Protection of... AGENCIES FOR USE OF PESTICIDES UNDER EMERGENCY CONDITIONS Specific, Quarantine, and Public Health Exemptions § 166.32 Reporting and recordkeeping requirements for specific, quarantine, and public...

  19. 40 CFR 166.32 - Reporting and recordkeeping requirements for specific, quarantine, and public health exemptions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requirements for specific, quarantine, and public health exemptions. 166.32 Section 166.32 Protection of... AGENCIES FOR USE OF PESTICIDES UNDER EMERGENCY CONDITIONS Specific, Quarantine, and Public Health Exemptions § 166.32 Reporting and recordkeeping requirements for specific, quarantine, and public...

  20. 29 CFR 1926.452 - Additional requirements applicable to specific types of scaffolds.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... scaffolds. 1926.452 Section 1926.452 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND... Scaffolds § 1926.452 Additional requirements applicable to specific types of scaffolds. In addition to the applicable requirements of § 1926.451, the following requirements apply to the specific types of...

  1. 29 CFR 1926.452 - Additional requirements applicable to specific types of scaffolds.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... scaffolds. 1926.452 Section 1926.452 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND... Scaffolds § 1926.452 Additional requirements applicable to specific types of scaffolds. In addition to the applicable requirements of § 1926.451, the following requirements apply to the specific types of...

  2. 29 CFR 1926.452 - Additional requirements applicable to specific types of scaffolds.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... scaffolds. 1926.452 Section 1926.452 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND... Scaffolds § 1926.452 Additional requirements applicable to specific types of scaffolds. In addition to the applicable requirements of § 1926.451, the following requirements apply to the specific types of...

  3. 29 CFR 1926.452 - Additional requirements applicable to specific types of scaffolds.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... scaffolds. 1926.452 Section 1926.452 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND... Scaffolds § 1926.452 Additional requirements applicable to specific types of scaffolds. In addition to the applicable requirements of § 1926.451, the following requirements apply to the specific types of...

  4. 29 CFR 1926.452 - Additional requirements applicable to specific types of scaffolds.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... scaffolds. 1926.452 Section 1926.452 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND... Scaffolds § 1926.452 Additional requirements applicable to specific types of scaffolds. In addition to the applicable requirements of § 1926.451, the following requirements apply to the specific types of...

  5. Reference manual for a Requirements Specification Language (RSL), version 2.0

    NASA Technical Reports Server (NTRS)

    Fisher, Gene L.; Cohen, Gerald C.

    1993-01-01

    This report is a Reference Manual for a general-purpose Requirements Specification Language, RSL. The purpose of RSL is to specify precisely the external structure of a mechanized system and to define requirements that the system must meet. A system can be comprised of a mixture of hardware, software, and human processing elements. RSL is a hybrid of features found in several popular requirements specification languages and includes constructs for formal mathematical specification.

  6. Significance of Soft Zone Sediments at the SRS

    SciTech Connect

    Aadland, R.K.

    2000-02-03

    The purpose of this report is to provide information on the origin, extent and stability of ''soft zones'' in the carbonate bearing strata at the Savannah River Site (SRS). As part of this study, a comprehensive historical compendium of how soft zones have been addressed during the past 47 years at SRS is reviewed.

  7. Functions and Requirements and Specifications for Replacement of the Computer Automated Surveillance System (CASS)

    SciTech Connect

    SCAIEF, C.C.

    1999-12-16

    This functions, requirements and specifications document defines the baseline requirements and criteria for the design, purchase, fabrication, construction, installation, and operation of the system to replace the Computer Automated Surveillance System (CASS) alarm monitoring.

  8. 24 CFR 200.936 - Supplementary specific procedural requirements under HUD building products certification program...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... requirements under HUD building products certification program for solid fuel type room heaters and fireplace... Supplementary specific procedural requirements under HUD building products certification program for solid fuel... fireplace stoves certified under the HUD Building Products Certification Program shall be...

  9. 24 CFR 200.936 - Supplementary specific procedural requirements under HUD building products certification program...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... requirements under HUD building products certification program for solid fuel type room heaters and fireplace... Supplementary specific procedural requirements under HUD building products certification program for solid fuel... fireplace stoves certified under the HUD Building Products Certification Program shall be...

  10. 24 CFR 200.936 - Supplementary specific procedural requirements under HUD building products certification program...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... requirements under HUD building products certification program for solid fuel type room heaters and fireplace... Supplementary specific procedural requirements under HUD building products certification program for solid fuel... fireplace stoves certified under the HUD Building Products Certification Program shall be...

  11. 24 CFR 200.936 - Supplementary specific procedural requirements under HUD building products certification program...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... requirements under HUD building products certification program for solid fuel type room heaters and fireplace... Supplementary specific procedural requirements under HUD building products certification program for solid fuel... fireplace stoves certified under the HUD Building Products Certification Program shall be...

  12. Nuclear criticality safety evaluation of SRS 9971 shipping package. [SRS (Savannah River Site)

    SciTech Connect

    Vescovi, P.J.

    1993-02-01

    This evaluation is requested to revise the criticality evaluation used to generate Chapter 6 (Criticality Evaluation) of the Safety Analysis Report for Packaging (SARP) for shipment Of UO[sub 3] product from the Uranium Solidification Facility (USF) in the SRS 9971 shipping package. The pertinent document requesting this evaluation is included as Attachment I. The results of the evaluation are given in Attachment II which is written as Chapter 6 of a NRC format SARP.

  13. American College of Radiology (ACR) and American Society for Radiation Oncology (ASTRO) Practice Guideline for the Performance of Stereotactic Radiosurgery (SRS)

    PubMed Central

    Seung, Steven K.; Larson, David A.; Galvin, James M.; Mehta, Minesh P.; Potters, Louis; Schultz, Christopher J.; Yajnik, Santosh V.; Hartford, Alan C.; Rosenthal, Seth A.

    2014-01-01

    American College of Radiology and American Society for Radiation Oncology Practice Guideline for the Performance of Stereotactic Radiosurgery (SRS). SRS is a safe and efficacious treatment option of a variety of benign and malignant disorders involving intracranial structures and selected extracranial lesions. SRS involves a high dose of ionizing radiation with a high degree of precision and spatial accuracy. A quality SRS program requires a multidisciplinary team involved in the patient management. Organization, appropriate staffing, and careful adherence to detail and to established SRS standards is important to ensure operational efficiency and to improve the likelihood of procedural success. A collaborative effort of the American College of Radiology and American Society for Therapeutic Radiation Oncology has produced a practice guideline for SRS. The guideline defines the qualifications and responsibilities of all the involved personnel, including the radiation oncologist, neurosurgeon, and qualified medical physicist. Quality assurance is essential for safe and accurate delivery of treatment with SRS. Quality assurance issues for the treatment unit, stereotactic accessories, medical imaging, and treatment-planning system are presented and discussed. Adherence to these practice guidelines can be part of ensuring quality and patient safety in a successful SRS program. PMID:23681017

  14. American College of Radiology (ACR) and American Society for Radiation Oncology (ASTRO) Practice Guideline for the Performance of Stereotactic Radiosurgery (SRS).

    PubMed

    Seung, Steven K; Larson, David A; Galvin, James M; Mehta, Minesh P; Potters, Louis; Schultz, Christopher J; Yajnik, Santosh V; Hartford, Alan C; Rosenthal, Seth A

    2013-06-01

    American College of Radiology and American Society for Radiation Oncology Practice Guideline for the Performance of Stereotactic Radiosurgery (SRS). SRS is a safe and efficacious treatment option of a variety of benign and malignant disorders involving intracranial structures and selected extracranial lesions. SRS involves a high dose of ionizing radiation with a high degree of precision and spatial accuracy. A quality SRS program requires a multidisciplinary team involved in the patient management. Organization, appropriate staffing, and careful adherence to detail and to established SRS standards is important to ensure operational efficiency and to improve the likelihood of procedural success. A collaborative effort of the American College of Radiology and American Society for Therapeutic Radiation Oncology has produced a practice guideline for SRS. The guideline defines the qualifications and responsibilities of all the involved personnel, including the radiation oncologist, neurosurgeon, and qualified medical physicist. Quality assurance is essential for safe and accurate delivery of treatment with SRS. Quality assurance issues for the treatment unit, stereotactic accessories, medical imaging, and treatment-planning system are presented and discussed. Adherence to these practice guidelines can be part of ensuring quality and patient safety in a successful SRS program.

  15. 10 CFR 810.12 - Information required in an application for specific authorization.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Information required in an application for specific authorization. 810.12 Section 810.12 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.12 Information required in an application for specific authorization. Each application...

  16. 10 CFR 810.12 - Information required in an application for specific authorization.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Information required in an application for specific authorization. 810.12 Section 810.12 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.12 Information required in an application for specific authorization. Each application...

  17. 10 CFR 810.12 - Information required in an application for specific authorization.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Information required in an application for specific authorization. 810.12 Section 810.12 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.12 Information required in an application for specific authorization. Each application...

  18. 10 CFR 810.12 - Information required in an application for specific authorization.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Information required in an application for specific authorization. 810.12 Section 810.12 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.12 Information required in an application for specific authorization. Each application...

  19. 10 CFR 810.12 - Information required in an application for specific authorization.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Information required in an application for specific authorization. 810.12 Section 810.12 Energy DEPARTMENT OF ENERGY ASSISTANCE TO FOREIGN ATOMIC ENERGY ACTIVITIES § 810.12 Information required in an application for specific authorization. Each application...

  20. 46 CFR 91.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Plans and specifications required for new construction. 91.55-5 Section 91.55-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CARGO AND MISCELLANEOUS VESSELS INSPECTION AND CERTIFICATION Plan Approval § 91.55-5 Plans and specifications required for new construction. (a) General....

  1. 40 CFR 270.16 - Specific part B information requirements for tank systems.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Permit Application § 270.16 Specific part B information requirements for tank systems. Except as... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Specific part B information requirements for tank systems. 270.16 Section 270.16 Protection of Environment ENVIRONMENTAL PROTECTION...

  2. 46 CFR 62.35-50 - Tabulated monitoring and safety control requirements for specific systems.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... (CONTINUED) MARINE ENGINEERING VITAL SYSTEM AUTOMATION Requirements for Specific Types of Automated Vital... instrumentation, alarms, and safety controls required for specific types of systems are listed in Table 62.35-50... casing and uptakes Fire Burner flame Status Failure Burner auto trip (3) Burner seating...

  3. 46 CFR 62.35-50 - Tabulated monitoring and safety control requirements for specific systems.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... (CONTINUED) MARINE ENGINEERING VITAL SYSTEM AUTOMATION Requirements for Specific Types of Automated Vital... instrumentation, alarms, and safety controls required for specific types of systems are listed in Table 62.35-50... casing and uptakes Fire Burner flame Status Failure Burner auto trip (3) Burner seating...

  4. 78 FR 47015 - Software Requirement Specifications for Digital Computer Software Used in Safety Systems of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-02

    ... issued with a temporary identification as Draft Regulatory Guide, DG-1209 on August 22, 2012 (77 FR 50726... COMMISSION Software Requirement Specifications for Digital Computer Software Used in Safety Systems of... 1 of RG 1.172, ``Software Requirement Specifications for Digital Computer Software used in...

  5. 77 FR 50726 - Software Requirement Specifications for Digital Computer Software and Complex Electronics Used in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-22

    ... COMMISSION Software Requirement Specifications for Digital Computer Software and Complex Electronics Used in... Digital Computer Software and Complex Electronics used in Safety Systems of Nuclear Power Plants.'' The DG... Requirement Specifications for Digital Computer Software and Complex Electronics used in Safety Systems...

  6. Tools reference manual for a Requirements Specification Language (RSL), version 2.0

    NASA Technical Reports Server (NTRS)

    Fisher, Gene L.; Cohen, Gerald C.

    1993-01-01

    This report describes a general-purpose Requirements Specification Language, RSL. The purpose of RSL is to specify precisely the external structure of a mechanized system and to define requirements that the system must meet. A system can be comprised of a mixture of hardware, software, and human processing elements. RSL is a hybrid of features found in several popular requirements specification languages, such as SADT (Structured Analysis and Design Technique), PSL (Problem Statement Language), and RMF (Requirements Modeling Framework). While languages such as these have useful features for structuring a specification, they generally lack formality. To overcome the deficiencies of informal requirements languages, RSL has constructs for formal mathematical specification. These constructs are similar to those found in formal specification languages such as EHDM (Enhanced Hierarchical Development Methodology), Larch, and OBJ3.

  7. 49 CFR 179.301 - Individual specification requirements for multi-unit tank car tanks.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...-unit tank car tanks. 179.301 Section 179.301 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Multi-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.301 Individual specification requirements for multi-unit tank car tanks. (a) In addition...

  8. 49 CFR 179.101 - Individual specification requirements applicable to pressure tank car tanks.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... to pressure tank car tanks. 179.101 Section 179.101 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.101 Individual specification requirements applicable to pressure tank...

  9. 49 CFR 179.301 - Individual specification requirements for multi-unit tank car tanks.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...-unit tank car tanks. 179.301 Section 179.301 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Multi-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.301 Individual specification requirements for multi-unit tank car tanks. (a) In addition...

  10. 49 CFR 179.101 - Individual specification requirements applicable to pressure tank car tanks.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... to pressure tank car tanks. 179.101 Section 179.101 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.101 Individual specification requirements applicable to pressure tank...

  11. 49 CFR 179.201 - Individual specification requirements applicable to non-pressure tank car tanks.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... to non-pressure tank car tanks. 179.201 Section 179.201 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.201 Individual specification requirements applicable to non-pressure tank car tanks....

  12. 49 CFR 179.101 - Individual specification requirements applicable to pressure tank car tanks.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... to pressure tank car tanks. 179.101 Section 179.101 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.101 Individual specification requirements applicable to pressure tank...

  13. 49 CFR 179.201 - Individual specification requirements applicable to non-pressure tank car tanks.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... to non-pressure tank car tanks. 179.201 Section 179.201 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.201 Individual specification requirements applicable to non-pressure tank car tanks....

  14. 49 CFR 179.301 - Individual specification requirements for multi-unit tank car tanks.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...-unit tank car tanks. 179.301 Section 179.301 Transportation Other Regulations Relating to... MATERIALS REGULATIONS SPECIFICATIONS FOR TANK CARS Specifications for Multi-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.301 Individual specification requirements for multi-unit tank car tanks. (a)...

  15. 49 CFR 179.201 - Individual specification requirements applicable to non-pressure tank car tanks.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... to non-pressure tank car tanks. 179.201 Section 179.201 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.201 Individual specification requirements applicable to non-pressure tank car tanks....

  16. 49 CFR 179.101 - Individual specification requirements applicable to pressure tank car tanks.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... to pressure tank car tanks. 179.101 Section 179.101 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.101 Individual specification requirements applicable to pressure tank...

  17. 49 CFR 179.301 - Individual specification requirements for multi-unit tank car tanks.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...-unit tank car tanks. 179.301 Section 179.301 Transportation Other Regulations Relating to... (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Multi-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.301 Individual specification requirements for multi-unit tank car tanks. (a) In addition...

  18. TRANSFORMING THE SRS ENVIRONMENTAL BUSINESS: COMMUNICATION AND APPLIED PROJECT MANAGEMENT PRINCIPLES

    SciTech Connect

    Saldivar, E.

    2010-01-20

    A process for communicating information relating to core business functions that also encourages improving internal communications has been established at SRS. This process continues to grow and strengthen as the multiple Contractors, Regulators and DOE-SR relationships mature. A number of management communication tools have been initiated, retooled, rebooted or continued with enhancements to ensure appropriate information is communicated to all levels with environmental responsibility at SRS. The types of information that are the focus of this improved process are feedback from the customer and from informational exchange forums (i.e., Challenge Opportunity and Resolution (COR), SRS Regulatory Integration Team (SRIT), Environmental Quality Management Division (EQMD), Senior Environmental Managers Council (SEMC), etc.). These forums, SRS environmental functions centralization, and the creation of a Regulatory Integration process allows for cross-functional decision making, problem solving and information sharing that involves the field organizations, Environmental Compliance Authorities (ECA), Subject Matter Experts (SME), DOE and the Regulators. Numerous examples of effective decision-making and problem solving will be shared. Lessons Learned involving inadequate communications and the resulting impacts on the environment, customer satisfaction, and relationships will also be discussed. Additionally, the focus on improved communications also includes maintaining awareness of business activities. The tools being utilized to facilitate the continuing improvement of internal communications include weekly staff meetings for all individuals within the organization, quarterly ECA and SME meeting, quarterly Regulatory Integration & Environmental Services (RI&ES) All-Hands meetings hosted by the Director, bi-weekly EQMD and EQMD Lite meetings with the customer, bi-annual SRIT meetings, and COR meetings on an as need basis. In addition, an existing Required Reading Program

  19. CEMENTITIOUS GROUT FOR CLOSING SRS HIGH LEVEL WASTE TANKS - #12315

    SciTech Connect

    Langton, C.; Burns, H.; Stefanko, D.

    2012-01-10

    In 1997, the first two United States Department of Energy (US DOE) high level waste tanks (Tanks 17-F and 20-F: Type IV, single shell tanks) were taken out of service (permanently closed) at the Savannah River Site (SRS). In 2012, the DOE plans to remove from service two additional Savannah River Site (SRS) Type IV high-level waste tanks, Tanks 18-F and 19-F. These tanks were constructed in the late 1950's and received low-heat waste and do not contain cooling coils. Operational closure of Tanks 18-F and 19-F is intended to be consistent with the applicable requirements of the Resource Conservation and Recovery Act (RCRA) and the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) and will be performed in accordance with South Carolina Department of Health and Environmental Control (SCDHEC). The closure will physically stabilize two 4.92E+04 cubic meter (1.3 E+06 gallon) carbon steel tanks and isolate and stabilize any residual contaminants left in the tanks. The closure will also fill, physically stabilize and isolate ancillary equipment abandoned in the tanks. A Performance Assessment (PA) has been developed to assess the long-term fate and transport of residual contamination in the environment resulting from the operational closure of the F-Area Tank Farm (FTF) waste tanks. Next generation flowable, zero-bleed cementitious grouts were designed, tested, and specified for closing Tanks 18-F and 19-F and for filling the abandoned equipment. Fill requirements were developed for both the tank and equipment grouts. All grout formulations were required to be alkaline with a pH of 12.4 and chemically reduction potential (Eh) of -200 to -400 to stabilize selected potential contaminants of concern. This was achieved by including Portland cement and Grade 100 slag in the mixes, respectively. Ingredients and proportions of cementitious reagents were selected and adjusted, respectively, to support the mass placement strategy developed by closure

  20. U.S. Space Station Freedom Supplemental Reboost System (SRS) design and operational impacts

    NASA Astrophysics Data System (ADS)

    Winters, Brian A.; Winters, Donna M.

    1992-07-01

    Current designs of the U.S. Space Station Freedom Propulsion System require the capability to perform reboost, attitude control, and other orbit adjustment maneuvers as part of normal operations. In order to reduce the quantity of hydrazine consumed by the Primary Propulsion System, the propulsion system includes the capability to propulsively dispose waste gases that are generated by various station systems and experiments. The impulses generated by the Supplemental Reboost System (SRS) are used to offset orbital decay due to atmospheric drag and reduce propellant requirements. This paper presents the designs of the SRS including schematics of the waste gas collection architecture, various system and component characteristics, and methods of system operation. Estimates of waste gas quantities, propellant savings, and operational altitude impacts are provided.

  1. 75 FR 32158 - Information Collection; SRS Publications Evaluation Card

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-07

    ...; ] DEPARTMENT OF AGRICULTURE Forest Service Information Collection; SRS Publications Evaluation Card AGENCY: Forest Service, USDA. ACTION: Notice; request for comment. SUMMARY: In accordance with the Paperwork Reduction Act of 1995, the Forest Service is seeking comments from all interested individuals...

  2. A fast shutdown system for SRS (Savannah River Site) reactors

    SciTech Connect

    Baumann, N.P.

    1990-01-01

    Power has been sharply reduced at Savannah River Site (SRS) reactors in large part to ensure that no bulk boiling occurs during hypothesized loss of coolant accidents. A fast shutdown system is essential to regain much of this lost power. Computations and experiments indicate that a He-3 injection system will serve this function. Instrumented tests of a full system are planned for early 1991 for one of the SRS reactors. 4 refs., 7 figs., 1 tab.

  3. 41 CFR 51-6.6 - Request for waiver of specification requirement.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... central nonprofit agencies are encouraged to recommend changes to specification requirements or request waivers where there are opportunities to provide equal or improved products at a lower cost to...

  4. Software requirements specification for the program analysis and control system risk management module

    SciTech Connect

    SCHAEFER, J.C.

    1999-06-02

    TWR Program Analysis and Control System Risk Module is used to facilitate specific data processes surrounding the Risk Management program of the Tank Waste Retrieval environment. This document contains the Risk Management system requirements of the database system.

  5. 24 CFR 200.952 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... under the HUD building product standards and certification program for particleboard interior stair... Supplementary specific requirements under the HUD building product standards and certification program for... certification program. In the case of interior particleboard stair treads, testing and inspection shall...

  6. 24 CFR 200.952 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... under the HUD building product standards and certification program for particleboard interior stair... Supplementary specific requirements under the HUD building product standards and certification program for... certification program. In the case of interior particleboard stair treads, testing and inspection shall...

  7. 24 CFR 200.952 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... under the HUD building product standards and certification program for particleboard interior stair... Supplementary specific requirements under the HUD building product standards and certification program for... certification program. In the case of interior particleboard stair treads, testing and inspection shall...

  8. 24 CFR 200.952 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... under the HUD building product standards and certification program for particleboard interior stair... Supplementary specific requirements under the HUD building product standards and certification program for... certification program. In the case of interior particleboard stair treads, testing and inspection shall...

  9. 24 CFR 200.952 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... under the HUD building product standards and certification program for particleboard interior stair... Supplementary specific requirements under the HUD building product standards and certification program for... certification program. In the case of interior particleboard stair treads, testing and inspection shall...

  10. AMTD: Update of Engineering Specifications Derived from Science Requirements for Future UVOIR Space Telescopes

    NASA Technical Reports Server (NTRS)

    Stahl, H. Philip

    2014-01-01

    AMTD is using a Science Driven Systems Engineering approach to develop Engineering Specifications based on Science Measurement Requirements and Implementation Constraints. Science requirements meet the needs of both Exoplanet and General Astrophysics science. Engineering Specifications are guiding our effort to mature to TRL-6 the critical technologies needed to produce 4-m or larger flight-qualified UVOIR mirrors by 2018 so that a viable mission can be considered by the 2020 Decadal Review.

  11. Cementitious Grout for Closing SRS High Level Waste Tanks - 12315

    SciTech Connect

    Langton, C.A.; Stefanko, D.B.; Burns, H.H.; Waymer, J.; Mhyre, W.B.; Herbert, J.E.; Jolly, J.C. Jr.

    2012-07-01

    In 1997, the first two United States Department of Energy (US DOE) high level waste tanks (Tanks 17-F and 20-F: Type IV, single shell tanks) were taken out of service (permanently closed) at the Savannah River Site (SRS). In 2012, the DOE plans to remove from service two additional Savannah River Site (SRS) Type IV high-level waste tanks, Tanks 18-F and 19-F. These tanks were constructed in the late 1950's and received low-heat waste and do not contain cooling coils. Operational closure of Tanks 18-F and 19-F is intended to be consistent with the applicable requirements of the Resource Conservation and Recovery Act (RCRA) and the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) and will be performed in accordance with South Carolina Department of Health and Environmental Control (SCDHEC). The closure will physically stabilize two 4.92E+04 cubic meter (1.3 E+06 gallon) carbon steel tanks and isolate and stabilize any residual contaminants left in the tanks. Ancillary equipment abandoned in the tanks will also be filled to the extent practical. A Performance Assessment (PA) has been developed to assess the long-term fate and transport of residual contamination in the environment resulting from the operational closure of the F-Area Tank Farm (FTF) waste tanks. Next generation flowable, zero-bleed cementitious grouts were designed, tested, and specified for closing Tanks 18-F and 19-F and for filling the abandoned equipment. Fill requirements were developed for both the tank and equipment grouts. All grout formulations were required to be alkaline with a pH of 12.4 and to be chemically reducing with a reduction potential (Eh) of -200 to -400. Grouts with this chemistry stabilize potential contaminants of concern. This was achieved by including Portland cement and Grade 100 slag in the mixes, respectively. Ingredients and proportions of cementitious reagents were selected and adjusted to support the mass placement strategy developed by

  12. 24 CFR 200.955 - Supplementary specific requirements under the HUD building product standard and certification...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... under the HUD building product standard and certification program for fenestration products (windows and... Supplementary specific requirements under the HUD building product standard and certification program for... testing for a product shall be described in the specific building product certification program. In...

  13. 24 CFR 200.955 - Supplementary specific requirements under the HUD building product standard and certification...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... under the HUD building product standard and certification program for fenestration products (windows and... Supplementary specific requirements under the HUD building product standard and certification program for... testing for a product shall be described in the specific building product certification program. In...

  14. 24 CFR 200.955 - Supplementary specific requirements under the HUD building product standard and certification...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... under the HUD building product standard and certification program for fenestration products (windows and... Supplementary specific requirements under the HUD building product standard and certification program for... testing for a product shall be described in the specific building product certification program. In...

  15. 24 CFR 200.955 - Supplementary specific requirements under the HUD building product standard and certification...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... under the HUD building product standard and certification program for fenestration products (windows and... Supplementary specific requirements under the HUD building product standard and certification program for... testing for a product shall be described in the specific building product certification program. In...

  16. 24 CFR 200.955 - Supplementary specific requirements under the HUD building product standard and certification...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... under the HUD building product standard and certification program for fenestration products (windows and... Supplementary specific requirements under the HUD building product standard and certification program for... testing for a product shall be described in the specific building product certification program. In...

  17. 24 CFR 200.936 - Supplementary specific procedural requirements under HUD building products certification program...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... requirements under HUD building products certification program for solid fuel type room heaters and fireplace... Supplementary specific procedural requirements under HUD building products certification program for solid fuel type room heaters and fireplace stoves. (a) Applicable standards. Solid fuel type room heaters...

  18. 10 CFR 72.236 - Specific requirements for spent fuel storage cask approval and fabrication.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 2 2011-01-01 2011-01-01 false Specific requirements for spent fuel storage cask approval and fabrication. 72.236 Section 72.236 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND...

  19. 10 CFR 72.236 - Specific requirements for spent fuel storage cask approval and fabrication.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 2 2012-01-01 2012-01-01 false Specific requirements for spent fuel storage cask approval and fabrication. 72.236 Section 72.236 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND...

  20. 10 CFR 72.236 - Specific requirements for spent fuel storage cask approval and fabrication.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 2 2013-01-01 2013-01-01 false Specific requirements for spent fuel storage cask approval and fabrication. 72.236 Section 72.236 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND...

  1. 10 CFR 72.236 - Specific requirements for spent fuel storage cask approval and fabrication.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 2 2014-01-01 2014-01-01 false Specific requirements for spent fuel storage cask approval and fabrication. 72.236 Section 72.236 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND...

  2. 40 CFR 270.18 - Specific part B information requirements for waste piles.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requirements for waste piles. 270.18 Section 270.18 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Permit Application § 270.18 Specific part B information requirements for waste piles. Except as otherwise provided in § 264.1, owners and operators of facilities that store or treat hazardous waste in waste...

  3. 40 CFR 270.21 - Specific part B information requirements for landfills.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... requirements for landfills. 270.21 Section 270.21 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Permit Application § 270.21 Specific part B information requirements for landfills. Except as otherwise provided in § 264.1, owners and operators of facilities that dispose of hazardous waste in landfills...

  4. 40 CFR 270.21 - Specific part B information requirements for landfills.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... requirements for landfills. 270.21 Section 270.21 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Permit Application § 270.21 Specific part B information requirements for landfills. Except as otherwise provided in § 264.1, owners and operators of facilities that dispose of hazardous waste in landfills...

  5. 40 CFR 270.21 - Specific part B information requirements for landfills.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requirements for landfills. 270.21 Section 270.21 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Permit Application § 270.21 Specific part B information requirements for landfills. Except as otherwise provided in § 264.1, owners and operators of facilities that dispose of hazardous waste in landfills...

  6. 40 CFR 270.21 - Specific part B information requirements for landfills.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... requirements for landfills. 270.21 Section 270.21 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Permit Application § 270.21 Specific part B information requirements for landfills. Except as otherwise provided in § 264.1, owners and operators of facilities that dispose of hazardous waste in landfills...

  7. Treated effluent disposal system process control computer software requirements and specification

    SciTech Connect

    Graf, F.A. Jr.

    1994-06-03

    The software requirements for the monitor and control system that will be associated with the effluent collection pipeline system known as the 200 Area Treated Effluent Disposal System is covered. The control logic for the two pump stations and specific requirements for the graphic displays are detailed.

  8. Repeat Courses of Stereotactic Radiosurgery (SRS), Deferring Whole-Brain Irradiation, for New Brain Metastases After Initial SRS

    SciTech Connect

    Shultz, David B.; Modlin, Leslie A.; Jayachandran, Priya; Von Eyben, Rie; Gibbs, Iris C.; Choi, Clara Y.H.; Chang, Steven D.; Harsh, Griffith R.; Li, Gordon; Adler, John R.; Hancock, Steven L.; Soltys, Scott G.

    2015-08-01

    Purpose: To report the outcomes of repeat stereotactic radiosurgery (SRS), deferring whole-brain radiation therapy (WBRT), for distant intracranial recurrences and identify factors associated with prolonged overall survival (OS). Patients and Methods: We retrospectively identified 652 metastases in 95 patients treated with 2 or more courses of SRS for brain metastases, deferring WBRT. Cox regression analyzed factors predictive for OS. Results: Patients had a median of 2 metastases (range, 1-14) treated per course, with a median of 2 courses (range, 2-14) of SRS per patient. With a median follow-up after first SRS of 15 months (range, 3-98 months), the median OS from the time of the first and second course of SRS was 18 (95% confidence interval [CI] 15-24) and 11 months (95% CI 6-17), respectively. On multivariate analysis, histology, graded prognostic assessment score, aggregate tumor volume (but not number of metastases), and performance status correlated with OS. The 1-year cumulative incidence, with death as a competing risk, of local failure was 5% (95% CI 4-8%). Eighteen (24%) of 75 deaths were from neurologic causes. Nineteen patients (20%) eventually received WBRT. Adverse radiation events developed in 2% of SRS sites. Conclusion: Multiple courses of SRS, deferring WBRT, for distant brain metastases after initial SRS, seem to be a safe and effective approach. The graded prognostic assessment score, updated at each course, and aggregate tumor volume may help select patients in whom the deferral of WBRT might be most beneficial.

  9. ALTERNATE APPROACH TO HAZARD CATEGORIZATION FOR SALTSTONE FACILITY AT SRS

    SciTech Connect

    Roy, B.

    2009-04-28

    The Saltstone Facility at Savannah River Site (SRS) was originally segmented into two segments: the Saltstone Production Facility (SPF) and the Saltstone Disposal Facility (SDF). Based on the inventory of radionuclides available for release the SPF and SDF were categorized as Nonreactor Hazard Category (HC)-3. The hazard categorization recognized the SDF will contain contributions of radionuclides which would exceed the HC-2 Threshold Quantity (TQ) in the form of grout. However it was determined not to impact the facility hazard categorization based on the grout being in a solid, monolithic form which was not easily dispersible. But, the impact of a quantity of unset grout expected to be present at the vault following operation of the process was not addressed. A Potential Inadequacy in Safety Analysis (PISA) was later issued based on the hazard categorization determination for the facility not addressing unset grout. This initiated a re-evaluation of the accident scenarios within the hazards analysis. During this re-evaluation, the segmentation of the facility was challenged based on the potential interaction between facility segments; specifically, the leachate return line and the grout transfer line, which were considered separate segments, are located in close proximity at one point. such that for certain events (NPH as well as External Vehicle Impact) both could be damaged simultaneously and spill contents on the ground that could commingle. This would violate the guideline for segmentation. Therefore, the Hazard Categorization (HC) was reevaluated based on the facility being a single segment and including the additional unset grout as part of total inventory. This total inventory far exceeded the limit for HC-2 TQ and made the facility's initial categorization as HC-2. However, alternative analysis methodology based on credible release fractions allowed in DOE-STD-1027-92 (Ref.1) showed that the Saltstone facility could still be categorized as Hazard Category

  10. Treatment of SRS Tank 48H Simulants Using Fenton's Reagent

    SciTech Connect

    Taylor, PA

    2003-11-18

    High-level-waste Tank 48H at the Savannah River Site (SRS) contains about 50,000 lb of tetraphenylborate (TPB), which must be destroyed to return the tank to active service. Laboratory-scale tests were conducted to evaluate the use of Fenton's Reagent (hydrogen peroxide and a metal catalyst) to treat simulants of the Tank 48H waste. Samples of the treated slurry and the off-gas were analyzed to determine the reaction products. Process parameters developed earlier by AEA Technology were used for these tests; namely (for 500 mL of waste simulant), reduce pH to 7.5 with nitric acid, heat to boiling, add hydrogen peroxide at 1 mL/min for 1 h, reduce pH to 3.5, and add the remaining peroxide at 2 mL/min. These parameters were developed to minimize the formation of tarry materials during the early part of the reaction and to minimize the concentration of total organic carbon in the final treated slurry. The treated samples contained low concentrations of total organic carbon (TOC) and no detectable TPB. Tests using a mixture of iron and copper salts as the Fenton's catalyst had a lower TOC concentration in the final treated slurry than did tests that used a copper-only catalyst. TPB is known to hydrolyze to benzene, particularly at high temperature and low pH, and copper is known to increase the rate of hydrolysis. Significant amounts of benzene were present in the off-gas from the tests, especially during the early portion of the treatment, indicating that the hydrolysis reaction was occurring in parallel with the oxidation of the TPB by Fenton's reagent. For the reaction conditions used in these tests, approximately equal fractions of the TPB were converted to benzene and carbon dioxide. Minimizing the formation of benzene is important to SRS personnel; however, this consideration was not addressed in the AEA-recommended parameters, since they did not analyze for benzene in the off-gas. Smaller amounts of carbon monoxide and other organics were also produced. One test

  11. ENVIRONMENTAL ASSESSMENT FOR THE NPDES STORM WATER COMPLIANCE ALTERNATIVES AT THE SRS

    SciTech Connect

    Shedrow, C

    2006-11-01

    The U.S. Department of Energy (DOE) prepared this environmental assessment (EA) to evaluate the potential environmental impacts associated with proposed and alternative actions to achieve water quality permit compliance at 38 storm water outfalls located at the Savannah River Site (SRS) (Figure 1-1). Effluent monitoring data indicates that some of these outfalls may not presently comply with new National Pollutant Discharge Elimination System (NPDES) Storm Water General Permit effluent standards that became effective July 1, 2005 (SCR000000). The NPDES permit requires that best management practices (BMPs) be implemented and maintained, as necessary, to ensure that storm water discharges at SRS do not cause or contribute to the contravention of applicable state water quality standards (WQS).

  12. Application of PSA to storage of Pu at SRS

    SciTech Connect

    Lux, C.R.

    1995-12-01

    Pu is stored in a wide variety of physical forms and containers at the Savannah River Site (SRS). Probabilistic Safety Analysis (PSA) techniques are used to determine the risk associated with each of these storage modes and assist in identification of the controls necessary to minimize the risk. One storage method involves solids in exposed drum storage where the drums are vulnerable to external events, natural phenomena, and release of material due to weathering of the containers. Another storage method may involve liquids being processed inside the canyon facilities where the greatest risks are not from external events but from process upsets. PSA techniques have been particularly useful in the evaluation of criticality situations concerning Pu processing and storage. The applications include ``normal`` operating situations, problems following a seismic event, and the identification of potential problems during the decontamination and decommissioning of a facility. In this paper I would like to discuss two specific examples of the use of PSA techniques. The first involves the analysis of potential accidents in a Pu receipt and storage facility. The second example involves processing solutions that have the potential for experiencing an uncontrolled ``red oil`` reaction.

  13. Automation of Protein Crystallography Facilities at the SRS

    NASA Astrophysics Data System (ADS)

    Nave, C.; Buffey, S. G.; Bailey, M. W.; Kinder, S. H.; Ackroyd, K.; Duke, E. H. M.

    2004-05-01

    The main use of synchrotron radiation facilities by protein crystallographers is to collect the best possible diffraction data for reasonably well defined problems. The process is therefore susceptible to automation. Significant effort throughout the world is therefore being given to automate SR protein crystallography facilities so scientists can obtain high throughput, even if they are not expert in all the techniques. Sample changers and automatic goniometers are being developed at various synchrotron sites and are also becoming available commercially. Tracking the various samples through the process is a significant issue. At the SRS we are taking a modular approach to automation, with a mixture of commercial products and in-house developments. Effort is required to integrate the various components but this approach gives the advantage that incremental upgrades can be made as new developments occur. A description of both the hardware and control software is given together with initial experience of the system. The developments are placed in the broader context of structure determination of proteins.

  14. Benefits of a Unified LaSRS++ Simulation for NAS-Wide and High-Fidelity Modeling

    NASA Technical Reports Server (NTRS)

    Glaab, Patricia; Madden, Michael

    2014-01-01

    The LaSRS++ high-fidelity vehicle simulation was extended in 2012 to support a NAS-wide simulation mode. Since the initial proof-of-concept, the LaSRS++ NAS-wide simulation is maturing into a research-ready tool. A primary benefit of this new capability is the consolidation of the two modeling paradigms under a single framework to save cost, facilitate iterative concept testing between the two tools, and to promote communication and model sharing between user communities at Langley. Specific benefits of each type of modeling are discussed along with the expected benefits of the unified framework. Current capability details of the LaSRS++ NAS-wide simulations are provided, including the visualization tool, live data interface, trajectory generators, terminal routing for arrivals and departures, maneuvering, re-routing, navigation, winds, and turbulence. The plan for future development is also described.

  15. PILOT SCALE TESTING OF MONOSODIUM TITANATE MIXING FOR THE SRS SMALL COLUMN ION EXCHANGE PROCESS - 11224

    SciTech Connect

    Poirier, M.; Restivo, M.; Williams, M.; Herman, D.; Steeper, T.

    2011-01-25

    The Small Column Ion Exchange (SCIX) process is being developed to remove cesium, strontium, and select actinides from Savannah River Site (SRS) Liquid Waste using an existing waste tank (i.e., Tank 41H) to house the process. Savannah River National Laboratory (SRNL) is conducting pilot-scale mixing tests to determine the pump requirements for suspending monosodium titanate (MST), crystalline silicotitanate (CST), and simulated sludge. The purpose of this pilot scale testing is to determine the requirements for the pumps to suspend the MST particles so that they can contact the strontium and actinides in the liquid and be removed from the tank. The pilot-scale tank is a 1/10.85 linear scaled model of SRS Tank 41H. The tank diameter, tank liquid level, pump nozzle diameter, pump elevation, and cooling coil diameter are all 1/10.85 of their dimensions in Tank 41H. The pump locations correspond to the proposed locations in Tank 41H by the SCIX program (Risers B5 and B2 for two pump configurations and Risers B5, B3, and B1 for three pump configurations). The conclusions from this work follow: (i) Neither two standard slurry pumps nor two quad volute slurry pumps will provide sufficient power to initially suspend MST in an SRS waste tank. (ii) Two Submersible Mixer Pumps (SMPs) will provide sufficient power to initially suspend MST in an SRS waste tank. However, the testing shows the required pump discharge velocity is close to the maximum discharge velocity of the pump (within 12%). (iii) Three SMPs will provide sufficient power to initially suspend MST in an SRS waste tank. The testing shows the required pump discharge velocity is 66% of the maximum discharge velocity of the pump. (iv) Three SMPs are needed to resuspend MST that has settled in a waste tank at nominal 45 C for four weeks. The testing shows the required pump discharge velocity is 77% of the maximum discharge velocity of the pump. Two SMPs are not sufficient to resuspend MST that settled under these

  16. SRS BEDROCK PROBABILISTIC SEISMIC HAZARD ANALYSIS (PSHA) DESIGN BASIS JUSTIFICATION (U)

    SciTech Connect

    , R

    2005-12-14

    This represents an assessment of the available Savannah River Site (SRS) hard-rock probabilistic seismic hazard assessments (PSHAs), including PSHAs recently completed, for incorporation in the SRS seismic hazard update. The prior assessment of the SRS seismic design basis (WSRC, 1997) incorporated the results from two PSHAs that were published in 1988 and 1993. Because of the vintage of these studies, an assessment is necessary to establish the value of these PSHAs considering more recently collected data affecting seismic hazards and the availability of more recent PSHAs. This task is consistent with the Department of Energy (DOE) order, DOE O 420.1B and DOE guidance document DOE G 420.1-2. Following DOE guidance, the National Map Hazard was reviewed and incorporated in this assessment. In addition to the National Map hazard, alternative ground motion attenuation models (GMAMs) are used with the National Map source model to produce alternate hazard assessments for the SRS. These hazard assessments are the basis for the updated hard-rock hazard recommendation made in this report. The development and comparison of hazard based on the National Map models and PSHAs completed using alternate GMAMs provides increased confidence in this hazard recommendation. The alternate GMAMs are the EPRI (2004), USGS (2002) and a regional specific model (Silva et al., 2004). Weights of 0.6, 0.3 and 0.1 are recommended for EPRI (2004), USGS (2002) and Silva et al. (2004) respectively. This weighting gives cluster weights of .39, .29, .15, .17 for the 1-corner, 2-corner, hybrid, and Greens-function models, respectively. This assessment is judged to be conservative as compared to WSRC (1997) and incorporates the range of prevailing expert opinion pertinent to the development of seismic hazard at the SRS. The corresponding SRS hard-rock uniform hazard spectra are greater than the design spectra developed in WSRC (1997) that were based on the LLNL (1993) and EPRI (1988) PSHAs. The

  17. An introduction to requirements capture using PVS: Specification of a simple autopilot

    NASA Technical Reports Server (NTRS)

    Butler, Ricky W.

    1996-01-01

    This paper presents an introduction to capturing software requirements in the PVS formal language. The object of study is a simplified digital autopilot that was motivated in part by the mode control panel of NASA Langley's Boeing 737 research aircraft. The paper first presents the requirements for this autopilot in English and then steps the reader through a translation of these requirements into formal mathematics. Along the way deficiencies in the English specification are noted and repaired. Once completed, the formal PVS requirement is analyzed using the PVS theorem prover and shown to maintain an invariant over its state space.

  18. Procedures for evaluating technical specifications

    SciTech Connect

    Boccio, J.L.; Robinson, R.C.; Samanta, P.K.; Vesely, W.E.

    1987-01-01

    The Procedures for Evaluating Technical Specifications (PETS) Program being conducted by Brookhaven National Laboratory for the Office of Research, US Nuclear Regulatory Commission (NRC), is developing various risk-based approaches for modifying Technical Specifications (TS). This paper highlights the various risk-based issues being addressed by the program and presents examples that exemplify the use of PRA models for modifying TS, primarily elements of the Limiting Condition of Operation (LCOs) and Surveillance Requirements (SRs). PETS approaches to TS modification using more detailed analysis are presented in a companion paper.

  19. Towards the formal specification of the requirements and design of a processor interface unit: HOL listings

    NASA Technical Reports Server (NTRS)

    Fura, David A.; Windley, Phillip J.; Cohen, Gerald C.

    1993-01-01

    This technical report contains the HOL listings of the specification of the design and major portions of the requirements for a commercially developed processor interface unit (or PIU). The PIU is an interface chip performing memory interface, bus interface, and additional support services for a commercial microprocessor within a fault-tolerant computer system. This system, the Fault-Tolerant Embedded Processor (FTEP), is targeted towards applications in avionics and space requiring extremely high levels of mission reliability, extended maintenance-free operation, or both. This report contains the actual HOL listings of the PIU specification as it currently exists. Section two of this report contains general-purpose HOL theories that support the PIU specification. These theories include definitions for the hardware components used in the PIU, our implementation of bit words, and our implementation of temporal logic. Section three contains the HOL listings for the PIU design specification. Aside from the PIU internal bus (I-Bus), this specification is complete. Section four contains the HOL listings for a major portion of the PIU requirements specification. Specifically, it contains most of the definition for the PIU behavior associated with memory accesses initiated by the local processor.

  20. A Methodology for Writing High Quality Requirement Specifications and for Evaluating Existing Ones

    NASA Technical Reports Server (NTRS)

    Rosenberg, Linda; Hammer, Theodore

    1999-01-01

    Requirements development and management have always been critical in the implementation of software systems-engineers are unable to build what analysts can not define. It is generally accepted that the earlier in the life cycle potential risks are identified the easier it is to eliminate or manage the conditions that introduce that risk. Problems that are not found until testing are approximately 14 times more costly to fix than if the problem was found in the requirement phase. The requirements specification, as the first tangible representation of the capability to be produced, establishes the basis for all of the project's engineering management and assurance functions. If the quality of the requirements specification is poor it can give rise to risks in all areas of the project. Recently, automated tools have become available to support requirements management. The use of these tools not only provides support in the definition and tracing of requirements, but it also opens the door to effective use of metrics in characterizing and assessing the quality of the requirement specifications.

  1. A Methodology for Writing High Quality Requirements Specification and Evaluating Existing Ones

    NASA Technical Reports Server (NTRS)

    Rosenberg, Linda; Hammer, Theodore

    1999-01-01

    Requirements development and management have always been critical in the implementation of software systems; engineers are unable to build what analysts can't define. It is generally accepted that the earlier in the life cycle potential risks are identified the easier it is to eliminate or manage the conditions that introduce that risk. Problems that are not found until testing are approximately 14 times more costly to fix than if the problem was found in the requirement phase. The requirements specification, as the first tangible representation of the capability to be produced, establishes the basis for all of the project's engineering management and assurance functions. If the quality of the requirements specification is poor it can give rise to risks in all areas of the project. Recently, automated tools have become available to support requirements management. The use of these tools not only provides support in the definition and tracing of requirements, but it also opens the door to effective use of metrics in characterizing and assessing the quality of the requirement specifications.

  2. Shedding new light on lipid functions with CARS and SRS microscopy.

    PubMed

    Yu, Yong; Ramachandran, Prasanna V; Wang, Meng C

    2014-08-01

    Modern optical microscopy has granted biomedical scientists unprecedented access to the inner workings of a cell, and revolutionized our understanding of the molecular mechanisms underlying physiological and disease states. In spite of these advances, however, visualization of certain classes of molecules (e.g. lipids) at the sub-cellular level has remained elusive. Recently developed chemical imaging modalities - Coherent Anti-Stokes Raman Scattering (CARS) microscopy and Stimulated Raman Scattering (SRS) microscopy - have helped bridge this gap. By selectively imaging the vibration of a specific chemical group, these non-invasive techniques allow high-resolution imaging of individual molecules in vivo, and circumvent the need for potentially perturbative extrinsic labels. These tools have already been applied to the study of fat metabolism, helping uncover novel regulators of lipid storage. Here we review the underlying principle of CARS and SRS microscopy, and discuss the advantages and caveats of each technique. We also review recent applications of these tools in the study of lipids as well as other biomolecules, and conclude with a brief guide for interested researchers to build and use CARS/SRS systems for their own research. This article is part of a Special Issue entitled Tools to study lipid functions.

  3. Assessment of SRS radiological liquid and airborne contaminants and pathways

    SciTech Connect

    Jannik, G.T.

    1997-04-01

    This report compiles and documents the radiological critical-contaminant/critical-pathway analysis performed for SRS. The analysis covers radiological releases to the atmosphere and to surface water, which are the principal media that carry contaminants off site. During routine operations at SRS, limited amounts of radionuclides are released to the environment through atmospheric and/or liquid pathways. These releases potentially result in exposure to offsite people. Though the groundwater beneath an estimated 5 to 10 percent of SRS has been contaminated by radionuclides, there is no evidence that groundwater contaminated with these constituents has migrated offsite (Arnett, 1996). Therefore, with the notable exception of radiological source terms originating from shallow surface water migration into site streams, onsite groundwater was not considered as a potential exposure pathway to offsite people.

  4. Temperature coefficients of reactivity for the SRS Mark 22 assembly

    SciTech Connect

    George, D.L.; Frost, R.L. )

    1992-01-01

    The nuclear reactors at the Savannah River Site (SRS) are unique in design and operation. In contrast to commercial power reactors, the SRS reactors were designed for isotope production rather than power generation. The SRS reactors are cooled and moderated by heavy water at near-atmospheric pressure, and the fuel assemblies consist of concentric annualar tubes rather than the solid pins typically found in power reactors. These and other factors make the neutronic behavior of SRS reactors unique. Temperature coefficients of reactivity are a measure of the change in core reactivity resulting from a change in the temperature of the reactor components. These coefficients are used in safety analyses and for prediction of reactivity changes with control rod moves during reactor operations. This paper presents the results of an investigation of temperature coefficients for the Mark 22 assembly currently charged to the K Reactor. The Mark 22 assembly is the tritium-producing assembly currently in use at SRS. This assembly contains two concentric fuel tubes of enriched uranium-aluminum alloy located between two concentric target tubes of lithium-aluminum alloy. There are three active coolant channels (cooling both sides of each fuel tube) and two low-flow dead spaces at the center and outside of the assembly. Heavy water flows down the three coolant channels in the Mark 22 assemblies and then jets out into the moderator space (the heavy water region bewteen assemblies). Six regional temperature coefficients are calculated at SRS: fuel, target, coolant, dead space, moderator upflow, and moderator downflow. The first four coefficients correspond to regions of the assembly and are calculated using the GLASS infinite lattice transport code. The two moderator coefficients correspond to reactor core regions and are calculated using the GRIMHX three-dimensional finite lattice diffusion theory code. Assembly coefficients calculated by GLASS have been experimentally verified.

  5. SWS: accessing SRS sites contents through Web Services

    PubMed Central

    Romano, Paolo; Marra, Domenico

    2008-01-01

    Background Web Services and Workflow Management Systems can support creation and deployment of network systems, able to automate data analysis and retrieval processes in biomedical research. Web Services have been implemented at bioinformatics centres and workflow systems have been proposed for biological data analysis. New databanks are often developed by taking into account these technologies, but many existing databases do not allow a programmatic access. Only a fraction of available databanks can thus be queried through programmatic interfaces. SRS is a well know indexing and search engine for biomedical databanks offering public access to many databanks and analysis tools. Unfortunately, these data are not easily and efficiently accessible through Web Services. Results We have developed ‘SRS by WS’ (SWS), a tool that makes information available in SRS sites accessible through Web Services. Information on known sites is maintained in a database, srsdb. SWS consists in a suite of WS that can query both srsdb, for information on sites and databases, and SRS sites. SWS returns results in a text-only format and can be accessed through a WSDL compliant client. SWS enables interoperability between workflow systems and SRS implementations, by also managing access to alternative sites, in order to cope with network and maintenance problems, and selecting the most up-to-date among available systems. Conclusions Development and implementation of Web Services, allowing to make a programmatic access to an exhaustive set of biomedical databases can significantly improve automation of in-silico analysis. SWS supports this activity by making biological databanks that are managed in public SRS sites available through a programmatic interface. PMID:18387203

  6. 41 CFR 301-2.5 - What travel arrangements require specific authorization or prior approval?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 4 2010-07-01 2010-07-01 false What travel arrangements require specific authorization or prior approval? 301-2.5 Section 301-2.5 Public Contracts and Property Management Federal Travel Regulation System TEMPORARY DUTY (TDY) TRAVEL ALLOWANCES INTRODUCTION...

  7. 7 CFR 1724.54 - Requirements for RUS approval of plans and specifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 11 2012-01-01 2012-01-01 false Requirements for RUS approval of plans and specifications. 1724.54 Section 1724.54 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE ELECTRIC ENGINEERING, ARCHITECTURAL SERVICES AND DESIGN POLICIES AND PROCEDURES Electric...

  8. 14 CFR 380.32 - Specific requirements for operator-participant contracts.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Specific requirements for operator-participant contracts. 380.32 Section 380.32 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF... will furnish details of the insurance to participants who check the space provided for this purpose...

  9. A Skills Map: The General and Specific Competencies Required in Different Occupations. Progress Report.

    ERIC Educational Resources Information Center

    Gottfredson, Linda S.

    To aid in assessing the employability of individuals and of various groups of individuals in different types of occupations, the general abilities and specific skills required of workers in different occupations were studied. Data on a comprehensive set of occupations were obtained from the Dictionary of Occupational Titles (DOT) job ratings and…

  10. 49 CFR 171.23 - Requirements for specific materials and packagings transported under the ICAO Technical...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., may be filled with a gas in the United States and offered for transportation and transported for... transported under the ICAO Technical Instructions, IMDG Code, Transport Canada TDG Regulations, or the IAEA... International Transport Standards and Regulations § 171.23 Requirements for specific materials and...

  11. 7 CFR 1724.54 - Requirements for RUS approval of plans and specifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE ELECTRIC ENGINEERING, ARCHITECTURAL SERVICES AND DESIGN POLICIES AND PROCEDURES Electric System Design § 1724.54 Requirements for RUS approval of plans... specifications for transmission construction projects which are not based on RUS approved line design data or...

  12. 40 CFR 60.3019 - What site-specific documentation is required?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... procedures. (8) The waste management plan required under §§ 60.3010 through 60.3012. (9) Procedures for... Times for Other Solid Waste Incineration Units That Commenced Construction On or Before December 9, 2004 Model Rule-Operator Training and Qualification § 60.3019 What site-specific documentation is...

  13. 10 CFR 40.32 - General requirements for issuance of specific licenses.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false General requirements for issuance of specific licenses. 40.32 Section 40.32 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC LICENSING OF SOURCE MATERIAL License... applicant is qualified by reason of training and experience to use the source material for the...

  14. 49 CFR 171.23 - Requirements for specific materials and packagings transported under the ICAO Technical...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    .... Chemical oxygen generators must be approved, classed, described, packaged, and transported in accordance... transported under the ICAO Technical Instructions, IMDG Code, Transport Canada TDG Regulations, or the IAEA... International Transport Standards and Regulations § 171.23 Requirements for specific materials and...

  15. 7 CFR 1724.54 - Requirements for RUS approval of plans and specifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE ELECTRIC ENGINEERING, ARCHITECTURAL SERVICES AND DESIGN POLICIES AND PROCEDURES Electric System Design § 1724.54 Requirements for RUS approval of plans and specifications. The provisions of this section apply only to RUS financed electric...

  16. 24 CFR 200.942 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... requirements under HUD building product standards and certification program for carpet and carpet with attached... for carpet and carpet with attached cushion. (a) Applicable standards. (1) Carpet and carpet with... 1983)—Standard Specification for Rubber Cellular Cushion Used for Carpet or Rug Underlay; (vii)...

  17. 24 CFR 200.942 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... requirements under HUD building product standards and certification program for carpet and carpet with attached... for carpet and carpet with attached cushion. (a) Applicable standards. (1) Carpet and carpet with... 1983)—Standard Specification for Rubber Cellular Cushion Used for Carpet or Rug Underlay; (vii)...

  18. 24 CFR 200.942 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... requirements under HUD building product standards and certification program for carpet and carpet with attached... for carpet and carpet with attached cushion. (a) Applicable standards. (1) Carpet and carpet with... 1983)—Standard Specification for Rubber Cellular Cushion Used for Carpet or Rug Underlay; (vii)...

  19. 24 CFR 200.942 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... requirements under HUD building product standards and certification program for carpet and carpet with attached... for carpet and carpet with attached cushion. (a) Applicable standards. (1) Carpet and carpet with... 1983)—Standard Specification for Rubber Cellular Cushion Used for Carpet or Rug Underlay; (vii)...

  20. 49 CFR 173.472 - Requirements for exporting DOT Specification Type B and fissile packages.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Authority Certificate applying to that package design to the national competent authority of each country... B and fissile packages. 173.472 Section 173.472 Transportation Other Regulations Relating to...) Materials § 173.472 Requirements for exporting DOT Specification Type B and fissile packages. (a)...

  1. 46 CFR 127.110 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Plans and specifications required for new construction... for new construction. Each applicant for approval of plans and for an original Certificate of...) Ballast systems. (v) Fluid-driven power and control systems. (vi) Through-hull penetrations and...

  2. 46 CFR 127.110 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Plans and specifications required for new construction... for new construction. Each applicant for approval of plans and for an original Certificate of...) Ballast systems. (v) Fluid-driven power and control systems. (vi) Through-hull penetrations and...

  3. 41 CFR 51-6.6 - Request for waiver of specification requirement.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Government. (b) A nonprofit agency shall not request a waiver of a specification requirement except when it... to furnish the item. (e) The central nonprofit agency request for waiver shall be transmitted in... Committee, annotated to include a statement concerning the impact on the cost of producing the item if...

  4. 41 CFR 51-6.6 - Request for waiver of specification requirement.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Government. (b) A nonprofit agency shall not request a waiver of a specification requirement except when it... to furnish the item. (e) The central nonprofit agency request for waiver shall be transmitted in... Committee, annotated to include a statement concerning the impact on the cost of producing the item if...

  5. 24 CFR 200.954 - Supplementary specific requirements under the HUD building product standard and certification...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... under the HUD building product standard and certification program for construction adhesives for wood... product certification program. In the case of construction adhesives for field glued wood floor systems... § 200.954 Supplementary specific requirements under the HUD building product standard and...

  6. 24 CFR 200.954 - Supplementary specific requirements under the HUD building product standard and certification...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... under the HUD building product standard and certification program for construction adhesives for wood... product certification program. In the case of construction adhesives for field glued wood floor systems... § 200.954 Supplementary specific requirements under the HUD building product standard and...

  7. 24 CFR 200.943 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... under the HUD building product standards and certification program for the grademarking of lumber. 200... Supplementary specific requirements under the HUD building product standards and certification program for the... reference in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. It is available from the U.S. Department...

  8. 24 CFR 200.954 - Supplementary specific requirements under the HUD building product standard and certification...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... under the HUD building product standard and certification program for construction adhesives for wood... product certification program. In the case of construction adhesives for field glued wood floor systems... § 200.954 Supplementary specific requirements under the HUD building product standard and...

  9. 24 CFR 200.943 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... under the HUD building product standards and certification program for the grademarking of lumber. 200... Supplementary specific requirements under the HUD building product standards and certification program for the... reference in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. It is available from the U.S. Department...

  10. 24 CFR 200.943 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... under the HUD building product standards and certification program for the grademarking of lumber. 200... Supplementary specific requirements under the HUD building product standards and certification program for the... reference in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. It is available from the U.S. Department...

  11. 24 CFR 200.943 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... under the HUD building product standards and certification program for the grademarking of lumber. 200... Supplementary specific requirements under the HUD building product standards and certification program for the... reference in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. It is available from the U.S. Department...

  12. 24 CFR 200.943 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... under the HUD building product standards and certification program for the grademarking of lumber. 200... Supplementary specific requirements under the HUD building product standards and certification program for the... reference in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. It is available from the U.S. Department...

  13. 14 CFR 380.32 - Specific requirements for operator-participant contracts.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Specific requirements for operator-participant contracts. 380.32 Section 380.32 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF... will furnish details of the insurance to participants who check the space provided for this purpose...

  14. 24 CFR 200.937 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Supplementary specific procedural requirements under HUD building product standards and certification program for plastic bathtub units, plastic shower receptors and stalls, plastic lavatories, plastic water closet bowls and tanks. 200.937 Section 200.937 Housing and Urban...

  15. 40 CFR 270.16 - Specific part B information requirements for tank systems.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Specific part B information requirements for tank systems. 270.16 Section 270.16 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... system; (e) A description of materials and equipment used to provide external corrosion protection,...

  16. 40 CFR 270.16 - Specific part B information requirements for tank systems.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 27 2011-07-01 2011-07-01 false Specific part B information requirements for tank systems. 270.16 Section 270.16 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... system; (e) A description of materials and equipment used to provide external corrosion protection,...

  17. 40 CFR 270.16 - Specific part B information requirements for tank systems.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Specific part B information requirements for tank systems. 270.16 Section 270.16 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... system; (e) A description of materials and equipment used to provide external corrosion protection,...

  18. 40 CFR 270.17 - Specific part B information requirements for surface impoundments.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Specific part B information requirements for surface impoundments. 270.17 Section 270.17 Protection of Environment ENVIRONMENTAL PROTECTION... an engineering report describing how the surface impoundment is designed and is or will...

  19. 40 CFR 270.17 - Specific part B information requirements for surface impoundments.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Specific part B information requirements for surface impoundments. 270.17 Section 270.17 Protection of Environment ENVIRONMENTAL PROTECTION... an engineering report describing how the surface impoundment is designed and is or will...

  20. International Low Impact Docking System (iLIDS) Project Technical Requirements Specification, Revision F

    NASA Technical Reports Server (NTRS)

    Lewis, James L.

    2011-01-01

    The NASA Docking System (NDS) is NASA's implementation for the emerging International Docking System Standard (IDSS) using low impact docking technology. The NASA Docking System Project (NDSP) is the International Space Station (ISS) Program's project to produce the NDS, Common Docking Adapter (CDA) and Docking Hub. The NDS design evolved from the Low Impact Docking System (LIDS). The acronym international Low Impact Docking System (iLIDS) is also used to describe this system as well as the Government Furnished Equipment (GFE) project designing the NDS for the NDSP. NDS and iLIDS may be used interchangeability. This document will use the acronym iLIDS. Some of the heritage documentation and implementations (e.g., software command names, requirement identification (ID), figures, etc.) used on NDS will continue to use the LIDS acronym. This specification defines the technical requirements for the iLIDS GFE delivered to the NDSP by the iLIDS project. This document contains requirements for two iLIDS configurations, SEZ29101800-301 and SEZ29101800-302. Requirements with the statement, iLIDS shall, are for all configurations. Examples of requirements that are unique to a single configuration may be identified as iLIDS (-301) shall or iLIDS (-302) shall. Furthermore, to allow a requirement to encompass all configurations with an exception, the requirement may be designated as iLIDS (excluding -302) shall. Verification requirements for the iLIDS project are identified in the Verification Matrix (VM) provided in the iLIDS Verification and Validation Document, JSC-63966. The following definitions differentiate between requirements and other statements: Shall: This is the only verb used for the binding requirements. Should/May: These verbs are used for stating non-mandatory goals. Will: This verb is used for stating facts or declaration of purpose. A Definition of Terms table is provided in Appendix B to define those terms with specific tailored uses in this document.

  1. NAIP proteins are required for cytosolic detection of specific bacterial ligands in vivo.

    PubMed

    Rauch, Isabella; Tenthorey, Jeannette L; Nichols, Randilea D; Al Moussawi, Khatoun; Kang, James J; Kang, Chulho; Kazmierczak, Barbara I; Vance, Russell E

    2016-05-01

    NLRs (nucleotide-binding domain [NBD] leucine-rich repeat [LRR]-containing proteins) exhibit diverse functions in innate and adaptive immunity. NAIPs (NLR family, apoptosis inhibitory proteins) are NLRs that appear to function as cytosolic immunoreceptors for specific bacterial proteins, including flagellin and the inner rod and needle proteins of bacterial type III secretion systems (T3SSs). Despite strong biochemical evidence implicating NAIPs in specific detection of bacterial ligands, genetic evidence has been lacking. Here we report the use of CRISPR/Cas9 to generate Naip1(-/-) and Naip2(-/-) mice, as well as Naip1-6(Δ/Δ) mice lacking all functional Naip genes. By challenging Naip1(-/-) or Naip2(-/-) mice with specific bacterial ligands in vivo, we demonstrate that Naip1 is uniquely required to detect T3SS needle protein and Naip2 is uniquely required to detect T3SS inner rod protein, but neither Naip1 nor Naip2 is required for detection of flagellin. Previously generated Naip5(-/-) mice retain some residual responsiveness to flagellin in vivo, whereas Naip1-6(Δ/Δ) mice fail to respond to cytosolic flagellin, consistent with previous biochemical data implicating NAIP6 in flagellin detection. Our results provide genetic evidence that specific NAIP proteins function to detect specific bacterial proteins in vivo. PMID:27045008

  2. Towards the formal specification of the requirements and design of a processor interface unit

    NASA Technical Reports Server (NTRS)

    Fura, David A.; Windley, Phillip J.; Cohen, Gerald C.

    1993-01-01

    Work to formally specify the requirements and design of a Processor Interface Unit (PIU), a single-chip subsystem providing memory interface, bus interface, and additional support services for a commercial microprocessor within a fault-tolerant computer system, is described. This system, the Fault-Tolerant Embedded Processor (FTEP), is targeted towards applications in avionics and space requiring extremely high levels of mission reliability, extended maintenance free operation, or both. The approaches that were developed for modeling the PIU requirements and for composition of the PIU subcomponents at high levels of abstraction are described. These approaches were used to specify and verify a nontrivial subset of the PIU behavior. The PIU specification in Higher Order Logic (HOL) is documented in a companion NASA contractor report entitled 'Towards the Formal Specification of the Requirements and Design of a Processor Interfacs Unit - HOL Listings.' The subsequent verification approach and HOL listings are documented in NASA contractor report entitled 'Towards the Formal Verification of the Requirements and Design of a Processor Interface Unit' and NASA contractor report entitled 'Towards the Formal Verification of the Requirements and Design of a Processor Interface Unit - HOL Listings.'

  3. A discussion of higher order software concepts as they apply to functional requirements and specifications. [space shuttles and guidance

    NASA Technical Reports Server (NTRS)

    Hamilton, M.

    1973-01-01

    The entry guidance software functional requirements (requirements design phase), its architectural requirements (specifications design phase), and the entry guidance software verified code are discussed. It was found that the proper integration of designs at both the requirements and specifications levels are of high priority consideration.

  4. NASA specification for manufacturing and performance requirements of NASA standard aerospace nickel-cadmium cells

    NASA Technical Reports Server (NTRS)

    1988-01-01

    On November 25, 1985, the NASA Chief Engineer established a NASA-wide policy to maintain and to require the use of the NASA standard for aerospace nickel-cadmium cells and batteries. The Associate Administrator for Safety, Reliability, Maintainability, and Quality Assurance stated on December 29, 1986, the intent to retain the NASA standard cell usage policy established by the Office of the Chief Engineer. The current NASA policy is also to incorporate technological advances as they are tested and proven for spaceflight applications. This policy will be implemented by modifying the existing standard cells or by developing new NASA standards and their specifications in accordance with the NASA's Aerospace Battery Systems Program Plan. This NASA Specification for Manufacturing and Performance Requirements of NASA Standard Aerospace Nickel-Cadmium Cells is prepared to provide requirements for the NASA standard nickel-cadmium cell. It is an interim specification pending resolution of the separator material availability. This specification has evolved from over 15 years of nickel-cadmium cell experience by NASA. Consequently, considerable experience has been collected and cell performance has been well characterized from many years of ground testing and from in-flight operations in both geosynchronous (GEO) and low earth orbit (LEO) applications. NASA has developed and successfully used two standard flight qualified cell designs.

  5. Apical-basal polarity proteins are required cell-type specifically to direct photoreceptor morphogenesis.

    PubMed

    Hwa, Jennifer J; Clandinin, Thomas R

    2012-12-18

    Insect photoreceptor function is dependent on precise placement of the rhabdomeres, elaborated apical domains specialized for capturing light, within each facet of a compound eye. In Diptera, an asymmetric arrangement of rhabdomeres, combined with a particular pattern of axonal connections, enhances light sensitivity through the principle of neural superposition. To achieve the necessary retinal geometry, different photoreceptors (R cells) have distinct shapes. The Crumbs and Bazooka complexes play critical roles in directing rhabdomere development, but whether they might direct cell-type-specific apical architectures is unknown. We demonstrate that while mutations in Bazooka complex members cause pleiotropic morphogenesis defects in all R cell subtypes, Crumbs (Crb) and Stardust (Sdt) function cell autonomously to direct early stages in rhabdomere assembly in specific subsets of R cells. This requirement is reflected in the cell-type-specific expression of Crb protein and demonstrates that Sdt and Crb can act independently to similar effect. These two genes are also required for zonula adherens (ZA) assembly but display an unusual pattern of cellular redundancy for this function, as each gene is required in only one of two adjoining cells. Our results provide a direct link between fate specification and morphogenetic patterning and suggest a model for ZA assembly.

  6. The RNA Export Factor, Nxt1, Is Required for Tissue Specific Transcriptional Regulation

    PubMed Central

    Jiang, Jianqiao; White-Cooper, Helen

    2013-01-01

    The highly conserved, Nxf/Nxt (TAP/p15) RNA nuclear export pathway is important for export of most mRNAs from the nucleus, by interacting with mRNAs and promoting their passage through nuclear pores. Nxt1 is essential for viability; using a partial loss of function allele, we reveal a role for this gene in tissue specific transcription. We show that many Drosophila melanogaster testis-specific mRNAs require Nxt1 for their accumulation. The transcripts that require Nxt1 also depend on a testis-specific transcription complex, tMAC. We show that loss of Nxt1 leads to reduced transcription of tMAC targets. A reporter transcript from a tMAC-dependent promoter is under-expressed in Nxt1 mutants, however the same transcript accumulates in mutants if driven by a tMAC-independent promoter. Thus, in Drosophila primary spermatocytes, the transcription factor used to activate expression of a transcript, rather than the RNA sequence itself or the core transcription machinery, determines whether this expression requires Nxt1. We additionally find that transcripts from intron-less genes are more sensitive to loss of Nxt1 function than those from intron-containing genes and propose a mechanism in which transcript processing feeds back to increase activity of a tissue specific transcription complex. PMID:23754955

  7. SRS Software Verification Pre Operational and Startup Test

    SciTech Connect

    HILL, L.F.

    2000-10-18

    This document defines testing for the software used to control the Sodium Removal System (SRS). The testing is conducted off-line from the. physical plant by using a simulator built-in to the software. This provides verification of proper software operation prior to performing the operational acceptance tests with the actual plant hardware.

  8. Risk-Dominant Scenarios from Several SRS Facilities

    SciTech Connect

    Blanchard, A.

    1999-03-15

    This report and associated spreadsheets describe the SRS safety analysis provided for four selected transuranic storage and stabilization facilities. For each of the four sets of analysis, the bounding events in each frequency category are identified, key inputs and assumptions are stated, and final doses tabulated.

  9. Performance requirements of real-time continuous media computing: specification techniques and hardware/software implications

    NASA Astrophysics Data System (ADS)

    Krishnaswamy, Venkatesh; Subrahmanyam, P. A.

    1996-01-01

    Today's typical multimedia computer consists of a fast CPU and multimedia peripherals such as attached sound and video support hardware. In such a machine, the low-level audio and video handling may be performed by these peripherals while any significant processing on these media requires the intimate involvement of the CPU, main memory system and the operating system. Given that these components are not typically designed to meet the real-time requirements of continuous media, there are a number of constraints on the nature of multimedia applications that can be executed. To get around these constraints, demanding multimedia applications may typically be offloaded to attached peripheral compute engines which are customized to execute the applications. This approach requires dedicating resources to specific applications and suffers from the problem that such applications are not flexible integrated with the main system. We posit that new software and hardware technologies are needed to truly integrate real-time multimedia processing capabilities within a multimedia computer. The design of these technologies must be based on a thorough understanding of the timing and real-time computing requirements of various types of continuous media. Formal mechanisms are needed to express and analyze these requirements in order for multimedia operating systems to efficiently and correctly schedule the use of computing and communication resources. Indeed such mechanisms may be used to guide the specification and design of hardware and software processing component. In this paper, we present a framework for specifying the timing properties and computational requirements of continuous media. We discuss the usefulness of this framework in the context of some common audio and video data formats. We also discuss the impact of this framework on the design of resource scheduling mechanisms. Finally we offer some insights on designing audio/video processing engines based on real

  10. Benchmark specifications and data requirements for initial modeling of the China experimental fast reactor.

    SciTech Connect

    Fanning, T. H.; Nuclear Engineering Division

    2010-06-04

    A specification is proposed for an initial transient benchmark analysis of the China Experimental Fast Reactor design based on the analysis capabilities of the SAS4A/SASSYS-1 code. For the initial benchmark, a single-channel protected transient overpower accident is defined. Reactivity feedback coefficients will not be required and simplified material properties are recommended. This report also describes the data required for developing the modeling input. This data includes assembly geometry, reactor power distributions, kinetics and decay heat data, and material properties. Comparisons of benchmark results will take place at a future SAS4A/SASSYS-1 training meeting planned to occur at Argonne National Laboratory. Future benchmark specifications will be planned to expand upon this initial model to include more complex reactivity feedback models, material properties, additional assembly geometry, and primary and intermediate coolant systems.

  11. Testing Requirements to Manage Data Exchange Specifications in Enterprise Integration - A Schema Design Quality Focus.

    SciTech Connect

    Kulvatunyou, Boonserm; Ivezic, Nenad; Buhwan, Jeong

    2004-07-01

    In this paper, we describe the requirements to test W3C XML Schema usage when defining message schemas for data exchange in any large and evolving enterprise integration project. We then decompose the XML Schema testing into four (4) aspects including the message schema conformance to the XML Schema specification grammar, the message schema conformance to the XML Schema specification semantics, the message schema conformance to design quality testing, and canonical semantics testing of the message schema. We describe these four testing aspects in some detail and point to other related efforts. We further focus to provide some technical details for the message schema design quality testing. As a future work, we describe the requirements for canonical semantics testing and potential solution approaches. Finally, we describe an implementation architecture for the message schema design quality testing.

  12. STATS SRS v11.0

    SciTech Connect

    Piscotty, M A; Nazario, O L

    2007-06-20

    The objective of this project is the delivery of an application that will provide a unified, web-based system for collecting, verifying and analyzing the achievements for Laboratory employees. The application will enable individual Directorates to manage and report achievement record data for their employees using an LLNL standard web browser. In addition, cross directorate data reporting and analysis will be available for such organizations as LSTO and programmatic directorates. This system is intended to store reference data and metadata for employee achievements. Abstracts and entire publications will not be stored in this system.Directorates are expected to use this system at all levels of management in preparing for Annual Self-Assessments, peer reviews, LDRD reviews, work force reviews, performance appraisals, and requests from sponsors. This document represents the primary deliverable for the Requirements Definition stage of system development. As part of a successful Requirements Definition, this document provides the development staff, the project sponsor, and the user community with a clear understanding of the product's operational, data, and other requirements. With this understanding, the development staff will take the opportunity to refine estimates regarding the cost, schedule, and deliverables reflected in it.

  13. 46 CFR 62.35-50 - Tabulated monitoring and safety control requirements for specific systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... reference; see 46 CFR 62.05-1) Part 4-9-4, tables 7A and 8. 2 See ABS Steel Vessel Rules Part 4-9-4, tables...) of this chapter. 8 See ABS Steel Vessel Rules Part 4-9-4, Table 8; and 46 CFR 58.10-15(f). 9 See ABS... (CONTINUED) MARINE ENGINEERING VITAL SYSTEM AUTOMATION Requirements for Specific Types of Automated...

  14. Gamma Ray Observatory (GRO) dynamics simulator requirements and mathematical specifications, revision 1

    NASA Technical Reports Server (NTRS)

    Harman, R.; Blejer, D.

    1990-01-01

    The requirements and mathematical specifications for the Gamma Ray Observatory (GRO) Dynamics Simulator are presented. The complete simulator system, which consists of the profie subsystem, simulation control and input/output subsystem, truth model subsystem, onboard computer model subsystem, and postprocessor, is described. The simulator will be used to evaluate and test the attitude determination and control models to be used on board GRO under conditions that simulate the expected in-flight environment.

  15. Soil Surface Seismic Hazard and Design Basis Guideline for PC 1 and 2 SRS Facilities

    SciTech Connect

    Lee, R.C.

    2000-04-26

    This report describes the development of Savannah River Site (SRS) soil seismic hazard for oscillator frequencies of 1, 2, 5, and 10 Hz. This soil surface hazard supersedes all previous soil hazard analyses conducted for the SRS.

  16. Specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation

    PubMed Central

    Baker, Charli D.; Basu Ball, Writoban; Pryce, Erin N.; Gohil, Vishal M.

    2016-01-01

    Mitochondrial membrane phospholipid composition affects mitochondrial function by influencing the assembly of the mitochondrial respiratory chain (MRC) complexes into supercomplexes. For example, the loss of cardiolipin (CL), a signature non–bilayer-forming phospholipid of mitochondria, results in disruption of MRC supercomplexes. However, the functions of the most abundant mitochondrial phospholipids, bilayer-forming phosphatidylcholine (PC) and non–bilayer-forming phosphatidylethanolamine (PE), are not clearly defined. Using yeast mutants of PE and PC biosynthetic pathways, we show a specific requirement for mitochondrial PE in MRC complex III and IV activities but not for their formation, whereas loss of PC does not affect MRC function or formation. Unlike CL, mitochondrial PE or PC is not required for MRC supercomplex formation, emphasizing the specific requirement of CL in supercomplex assembly. Of interest, PE biosynthesized in the endoplasmic reticulum (ER) can functionally substitute for the lack of mitochondrial PE biosynthesis, suggesting the existence of PE transport pathway from ER to mitochondria. To understand the mechanism of PE transport, we disrupted ER–mitochondrial contact sites formed by the ERMES complex and found that, although not essential for PE transport, ERMES facilitates the efficient rescue of mitochondrial PE deficiency. Our work highlights specific roles of non–bilayer-forming phospholipids in MRC function and formation. PMID:27226479

  17. Entry of Bluetongue Virus Capsid Requires the Late Endosome-specific Lipid Lysobisphosphatidic Acid*

    PubMed Central

    Patel, Avnish; Mohl, Bjorn-Patrick; Roy, Polly

    2016-01-01

    The entry of viruses into host cells is one of the key processes of infection. The mechanisms of cellular entry for enveloped virus have been well studied. The fusion proteins as well as the facilitating cellular lipid factors involved in the viral fusion entry process have been well characterized. The process of non-enveloped virus cell entry, in comparison, remains poorly defined, particularly for large complex capsid viruses of the family Reoviridae, which comprises a range of mammalian pathogens. These viruses enter cells without the aid of a limiting membrane and thus cannot fuse with host cell membranes to enter cells. Instead, these viruses are believed to penetrate membranes of the host cell during endocytosis. However, the molecular mechanism of this process is largely undefined. Here we show, utilizing an in vitro liposome penetration assay and cell biology, that bluetongue virus (BTV), an archetypal member of the Reoviridae, utilizes the late endosome-specific lipid lysobisphosphatidic acid for productive membrane penetration and viral entry. Further, we provide preliminary evidence that lipid lysobisphosphatidic acid facilitates pore expansion during membrane penetration, suggesting a mechanism for lipid factor requirement of BTV. This finding indicates that despite the lack of a membrane envelope, the entry process of BTV is similar in specific lipid requirements to enveloped viruses that enter cells through the late endosome. These results are the first, to our knowledge, to demonstrate that a large non-enveloped virus of the Reoviridae has specific lipid requirements for membrane penetration and host cell entry. PMID:27036941

  18. Requirement for additional treatment for dogs with atopic dermatitis undergoing allergen-specific immunotherapy.

    PubMed

    Colombo, S; Hill, P B; Shaw, D J; Thoday, K L

    2007-06-23

    Allergen-specific immunotherapy (ASIT) is one of the main treatments for atopic dermatitis in dogs, but it often requires additional treatments such as antibacterial and antifungal therapy for secondary bacterial and yeast infections, or antipruritic drugs to control the clinical signs or treat the adverse effects of the immunotherapy. Twenty-seven dogs enrolled in a study of ASIT were clinically assessed four times over a period of nine months; their requirement for treatment for secondary bacterial and yeast infections, for the administration of glucocorticoids as additional antipruritic therapy, and for the treatment of any adverse effects of the ASIT were evaluated. Twenty (74 per cent) of the dogs were treated for superficial bacterial pyoderma, 18 (66.6 per cent) required treatment for Malassezia species dermatitis on one or more occasions, eight (29.6 per cent) required treatment for otitis externa due to Malassezia species or bacteria, and eight required glucocorticoids to control their clinical signs. Five (18.5 per cent) of the dogs experienced adverse effects due to the ASIT and two required treatment with antihistamines (H1 receptor antagonists) in order to continue with the ASIT. PMID:17586789

  19. Glia-derived neurons are required for sex-specific learning in C. elegans

    PubMed Central

    Sammut, Michele; Cook, Steven J.; Nguyen, Ken C.Q.; Felton, Terry; Hall, David H.; Emmons, Scott W.

    2015-01-01

    Sex differences in behaviour extend to cognitive-like processes such as learning but the underlying dimorphisms in neural circuit development and organization that generate these behavioural differences are largely unknown. Here we define at the single-cell level, from development, through neural circuit connectivity, to function, the neural basis of a sex-specific learning in the nematode C. elegans. We show that sexual conditioning, a form of associative learning, requires a pair of male-specific interneurons whose progenitors are fully differentiated glia. These neurons are born during sexual maturation and incorporated into pre-exisiting sex-shared circuits to couple chemotactic responses to reproductive priorities. Our findings reveal a general role for glia as neural progenitors across metazoan taxa and demonstrate that the addition of sex-specific neuron types to brain circuits during sexual maturation is an important mechanism for the generation of sexually dimorphic plasticity in learning. PMID:26469050

  20. AMTD: Update of Engineering Specifications Derived from Science Requirements for Future UVOIR Space Telescopes

    NASA Technical Reports Server (NTRS)

    Stahl, H. Philip; Postman, Marc; Mosier, Gary; Smith, W. Scott; Blaurock, Carl; Ha, Kong; Stark, Christopher C.

    2014-01-01

    The Advance Mirror Technology Development (AMTD) project is in Phase 2 of a multiyear effort, initiated in FY12, to mature by at least a half TRL step six critical technologies required to enable 4 meter or larger UVOIR space telescope primary mirror assemblies for both general astrophysics and ultra-high contrast observations of exoplanets. AMTD uses a science-driven systems engineering approach. We mature technologies required to enable the highest priority science AND provide a high-performance low-cost low-risk system. To give the science community options, we are pursuing multiple technology paths. A key task is deriving engineering specifications for advanced normal-incidence monolithic and segmented mirror systems needed to enable both general astrophysics and ultra-high contrast observations of exoplanets missions as a function of potential launch vehicles and their mass and volume constraints. A key finding of this effort is that the science requires an 8 meter or larger aperture telescope

  1. Enterprise SRS: Leveraging Ongoing Operations to Advance National Programs - 13108

    SciTech Connect

    Marra, J.E.; Murray, A.M.; McGuire, P.W.; Wheeler, V.B.

    2013-07-01

    The SRS is re-purposing its vast array of assets to solve future national issues regarding environmental stewardship, national security, and clean energy. The vehicle for this transformation is Enterprise SRS which presents a new, strategic view of SRS as a united endeavor for 'all things nuclear' as opposed to a group of distinct and separate entities with individual missions and organizations. Key among the Enterprise SRS strategic initiatives is the integration of research into facilities in conjunction with ongoing missions to provide researchers from other national laboratories, academic institutions, and commercial entities the opportunity to demonstrate their technologies in a relevant environment and scale prior to deployment. To manage that integration of research demonstrations into site facilities, The DOE Savannah River Operations Office, Savannah River Nuclear Solutions, and the Savannah River National Laboratory (SRNL) have established the Center for Applied Nuclear Materials Processing and Engineering Research (CANMPER). The key objective of this initiative is to bridge the gap between promising transformational nuclear materials management advancements and large-scale deployment of the technology by leveraging SRS assets (e.g. facilities, staff, and property) for those critical engineering-scale demonstrations necessary to assure the successful deployment of new technologies. CANMPER will coordinate the demonstration of R and D technologies and serve as the interface between the engineering-scale demonstration and the R and D programs, essentially providing cradle-to-grave support to the R and D team during the demonstration. While the initial focus of CANMPER will be on the effective use of SRS assets for these demonstrations, CANMPER also will work with research teams to identify opportunities to perform R and D demonstrations at other facilities. Unique to this approach is the fact that these SRS assets will continue to accomplish DOE's critical

  2. Stereotactic radiosurgery (SRS) in the modern management of patients with brain metastases

    PubMed Central

    Soliman, Hany; Das, Sunit; Larson, David A.; Sahgal, Arjun

    2016-01-01

    Stereotactic radiosurgery (SRS) is an established non-invasive ablative therapy for brain metastases. Early clinical trials with SRS proved that tumor control rates are superior to whole brain radiotherapy (WBRT) alone. As a result, WBRT plus SRS was widely adopted for patients with a limited number of brain metastases (“limited number” customarily means 1-4). Subsequent trials focused on answering whether WBRT upfront was necessary at all. Based on current randomized controlled trials (RCTs) and meta-analyses comparing SRS alone to SRS plus WBRT, adjuvant WBRT results in better intracranial control; however, at the expense of neurocognitive functioning and quality of life. These adverse effects of WBRT may also negatively impact on survival in younger patients. Based on the results of these studies, treatment has shifted to SRS alone in patients with a limited number of metastases. Additionally, RCTs are evaluating the role of SRS alone in patients with >4 brain metastases. New developments in SRS include fractionated SRS for large tumors and the integration of SRS with targeted systemic therapies that cross the blood brain barrier and/or stimulate an immune response. We present in this review the current high level evidence and rationale supporting SRS as the standard of care for patients with limited brain metastases, and emerging applications of SRS. PMID:26848525

  3. Screening Leishmania donovani complex-specific genes required for visceral disease.

    PubMed

    Zhang, Wen-Wei; Matlashewski, Greg

    2015-01-01

    Leishmania protozoan parasites are the causing agent of leishmaniasis. Depending on the infecting species, Leishmania infection can causes a wide variety of diseases such as self-healing cutaneous lesions by L. major and fatal visceral leishmaniasis by L. donovani and L. infantum. Comparison of the visceral disease causing L. infantum genome with cutaneous disease causing L. major and L. braziliensis genomes has identified 25 L. infantum (L. donovani complex) species-specific genes that are absent or pseudogenes in L. major and L. braziliensis. To investigate whether these L. donovani complex species-specific genes are involved in visceral infection, we cloned these genes from L. donovani and introduced them into L. major and then determined whether the transgenic L. major had an increased ability to survive in liver and spleen of BALB/c mice. Several of these L. donovani complex specific genes were found to significantly increase L. major survival in visceral organs in BALB/c mice including the A2 and Ld2834 genes, while down regulation of these genes in L. donovani by either antisense RNA or gene knockout dramatically reduced L. donovani virulence in BALB/c mice. This demonstrated that L. donovani complex species-specific genes play important roles in visceral infection. In this chapter, we describe procedures to screen L. donovani complex specific genes required for visceral infection by cross species transgenic expression, gene deletion targeting and measuring infection levels in mice. PMID:25388124

  4. The NIMA Kinase Is Required To Execute Stage-Specific Mitotic Functions after Initiation of Mitosis

    PubMed Central

    Govindaraghavan, Meera; Lad, Alisha A.

    2014-01-01

    The G2-M transition in Aspergillus nidulans requires the NIMA kinase, the founding member of the Nek kinase family. Inactivation of NIMA results in a late G2 arrest, while overexpression of NIMA is sufficient to promote mitotic events independently of cell cycle phase. Endogenously tagged NIMA-GFP has dynamic mitotic localizations appearing first at the spindle pole body and then at nuclear pore complexes before transitioning to within nuclei and the mitotic spindle and back at the spindle pole bodies at mitotic exit, suggesting that it functions sequentially at these locations. Since NIMA is indispensable for mitotic entry, it has been difficult to determine the requirement of NIMA for subaspects of mitosis. We show here that when NIMA is partially inactivated, although mitosis can be initiated, a proportion of cells fail to successfully generate two daughter nuclei. We further define the mitotic defects to show that normal NIMA function is required for the formation of a bipolar spindle, nuclear pore complex disassembly, completion of chromatin segregation, and the normal structural rearrangements of the nuclear envelope required to generate two nuclei from one. In the remaining population of cells that enter mitosis with inadequate NIMA, two daughter nuclei are generated in a manner dependent on the spindle assembly checkpoint, indicating highly penetrant defects in mitotic progression without sufficient NIMA activity. This study shows that NIMA is required not only for mitotic entry but also sequentially for successful completion of stage-specific mitotic events. PMID:24186954

  5. Evaluation of Background Mercury Concentrations in the SRS Groundwater System

    SciTech Connect

    Looney, B.B.

    1999-03-03

    Mercury analyses associated with the A-01 Outfall have highlighted the importance of developing an understanding of mercury in the Savannah River Site groundwater system and associated surface water streams. This activity is critical based upon the fact that the EPA Ambient Water Quality Criteria (AWQC) for this constituent is 0.012mg/L, a level that is well below conventional detection limits of 0.1 to 0.2 mg/L. A first step in this process is obtained by utilizing the existing investment in groundwater mercury concentrations (20,242 records) maintained in the SRS geographical information management system (GIMS) database. Careful use of these data provides a technically defensible initial estimate for total recoverable mercury in background and contaminated SRS wells.

  6. Nuclear criticality safety evaluation of SRS 9971 shipping package

    SciTech Connect

    Vescovi, P.J.

    1993-02-01

    This evaluation is requested to revise the criticality evaluation used to generate Chapter 6 (Criticality Evaluation) of the Safety Analysis Report for Packaging (SARP) for shipment Of UO{sub 3} product from the Uranium Solidification Facility (USF) in the SRS 9971 shipping package. The pertinent document requesting this evaluation is included as Attachment I. The results of the evaluation are given in Attachment II which is written as Chapter 6 of a NRC format SARP.

  7. Hybrid Microwave Treatment of SRS TRU and Mixed Wastes

    SciTech Connect

    Wicks, G.G.

    1999-11-18

    A new process, using hybrid microwave energy, has been developed as part of the Strategic Research and Development program and successfully applied to treatment of a wide variety of non-radioactive materials, representative of SRS transuranic (TRU) and mixed wastes. Over 35 simulated (non-radioactive) TRU and mixed waste materials were processed individually, as well as in mixed batches, using hybrid microwave energy, a new technology now being patented by Westinghouse Savannah River Company (WSRC).

  8. Specifications of the octupole magnets required for the ATF2 ultra-low ß* lattice

    SciTech Connect

    Marin, E.; Modena, M.; Tauchi, T.; Terunuma, N.; Tomas, R.; White, G.R.; /SLAC

    2014-05-28

    The Accelerator Test Facility 2 (ATF2) aims to test the novel chromaticity correction for higher chromaticity lattices as the one of CLIC. To this end the ATF2 ultra-low ß* lattice is designed to vertically focus the beam at the focal point or usually referred to as interaction point (IP), down to 23 nm. However when the measured multipole components of the ATF2 magnets are considered in the simulations, the evaluated spot sizes at the IP are well above the design value. The designed spot size is effectively recovered by inserting a pair of octupole magnets. In this note we address the technical specifications required for these octupole magnets.

  9. THERMAL MODELING ANALYSIS OF SRS 70 TON CASK

    SciTech Connect

    Lee, S.; Jordan, J.; Hensel, S.

    2011-03-08

    The primary objective of this work was to perform the thermal calculations to evaluate the Material Test Reactor (MTR) fuel assembly temperatures inside the SRS 70-Ton Cask loaded with various bundle powers. MTR fuel consists of HFBR, MURR, MIT, and NIST. The MURR fuel was used to develop a bounding case since it is the fuel with the highest heat load. The results will be provided for technical input for the SRS 70 Ton Cask Onsite Safety Assessment. The calculation results show that for the SRS 70 ton dry cask with 2750 watts total heat source with a maximum bundle heat of 670 watts and 9 bundles of MURR bounding fuel, the highest fuel assembly temperatures are below about 263 C. Maximum top surface temperature of the plastic cover is about 112 C, much lower than its melting temperature 260 C. For 12 bundles of MURR bounding fuel with 2750 watts total heat and a maximum fuel bundle of 482 watts, the highest fuel assembly temperatures are bounded by the 9 bundle case. The component temperatures of the cask were calculated by a three-dimensional computational fluid dynamics approach. The modeling calculations were performed by considering daily-averaged solar heat flux.

  10. Health effects of SRS non-radiological air emissions

    SciTech Connect

    Stewart, J.

    1997-06-16

    This report examines the potential health effects of non radiological emissions to the air resulting from operations at the Savannah River Site (SRS). The scope of this study was limited to the 55 air contaminants for which the US Environmental Protection Agency (EPA) has quantified risk by determining unit risk factors (excess cancer risks) and/or reference concentrations (deleterious non cancer risks). Potential health impacts have been assessed in relation to the maximally exposed individual. This is a hypothetical person who resides for a lifetime at the SRS boundary. The most recent (1994) quality assured SRS emissions data available were used. Estimated maximum site boundary concentrations of the air contaminants were calculated using air dispersion modeling and 24-hour and annual averaging times. For the emissions studied, the excess cancer risk was found to be less than the generally accepted risk level of 1 in 100,000 and, in most cases, was less than 1 in 1,000,000. Deleterious non cancer effects were also found to be very unlikely.

  11. 10 CFR 33.13 - Requirements for the issuance of a Type A specific license of broad scope.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Requirements for the issuance of a Type A specific license of broad scope. 33.13 Section 33.13 Energy NUCLEAR REGULATORY COMMISSION SPECIFIC DOMESTIC LICENSES OF BROAD SCOPE FOR BYPRODUCT MATERIAL Specific Licenses of Broad Scope § 33.13 Requirements for the issuance of a Type A specific license of...

  12. 10 CFR 33.13 - Requirements for the issuance of a Type A specific license of broad scope.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Requirements for the issuance of a Type A specific license of broad scope. 33.13 Section 33.13 Energy NUCLEAR REGULATORY COMMISSION SPECIFIC DOMESTIC LICENSES OF BROAD SCOPE FOR BYPRODUCT MATERIAL Specific Licenses of Broad Scope § 33.13 Requirements for the issuance of a Type A specific license of...

  13. 10 CFR 33.13 - Requirements for the issuance of a Type A specific license of broad scope.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Requirements for the issuance of a Type A specific license of broad scope. 33.13 Section 33.13 Energy NUCLEAR REGULATORY COMMISSION SPECIFIC DOMESTIC LICENSES OF BROAD SCOPE FOR BYPRODUCT MATERIAL Specific Licenses of Broad Scope § 33.13 Requirements for the issuance of a Type A specific license of...

  14. The efficacy of pneumococcal capsular polysaccharide-specific antibodies to serotype 3 Streptococcus pneumoniae requires macrophages.

    PubMed

    Fabrizio, Kevin; Manix, Catherine; Tian, Haijun; van Rooijen, Nico; Pirofski, Liise-anne

    2010-11-01

    The efficacy of antibody immunity against Streptococcus pneumoniae stems from the ability of opsonic, serotype (ST)-specific antibodies to pneumococcal capsular polysaccharide (PPS) to facilitate killing of the homologous ST by host phagocytes. However, PPS-specific antibodies have been identified that are protective in mice, but do not promote opsonic killing in vitro, raising the question of how they mediate protection in vivo. To probe this question, we investigated the dependence of antibody efficacy against lethal systemic (intraperitoneal, i.p.) infection with Streptococcus pneumoniae serotype 3 (ST3) on macrophages and neutrophils for the following PPS3-specific monoclonal antibodies (MAbs) in survival experiments in mice using a non-opsonic human IgM (A7), a non-opsonic mouse IgG1 (1E2) and an opsonic mouse IgG1 (5F6). The survival of A7- and PPS3-specific and isotype control MAb-treated neutrophil-depleted and neutrophil-sufficient and macrophage-depleted and macrophage-sufficient mice were determined after i.p. challenge with ST3 strains 6303 and WU2. Neutrophils were dispensable for A7 and the mouse MAbs to mediate protection in this model, but macrophages were required for the efficacy of A7 and optimal mouse MAb-mediated protection. For A7-treated mice, macrophage-depleted mice had higher blood CFU, cytokines and peripheral neutrophil levels than macrophage-sufficient mice, and macrophage-sufficient mice had lower tissue bacterial burdens than control MAb-treated mice. These findings demonstrate that macrophages contribute to opsonic and non-opsonic PPS3-specific MAb-mediated protection against ST3 infection by enhancing bacterial clearance and suggest that neutrophils do not compensate for the absence of macrophages in the model used in this study.

  15. Neurotransmitter Transporter-Like: A Male Germline-specific SLC6 Transporter Required for Drosophila Spermiogenesis

    PubMed Central

    Chatterjee, Nabanita; Rollins, Janet; Mahowald, Anthony P.; Bazinet, Christopher

    2011-01-01

    The SLC6 class of membrane transporters, known primarily as neurotransmitter transporters, is increasingly appreciated for its roles in nutritional uptake of amino acids and other developmentally specific functions. A Drosophila SLC6 gene, Neurotransmitter transporter-like (Ntl), is expressed only in the male germline. Mobilization of a transposon inserted near the 3′ end of the Ntl coding region yields male-sterile mutants defining a single complementation group. Germline transformation with Ntl cDNAs under control of male germline-specific control elements restores Ntl/Ntl homozygotes to normal fertility, indicating that Ntl is required only in the germ cells. In mutant males, sperm morphogenesis appears normal, with elongated, individualized and coiled spermiogenic cysts accumulating at the base of the testes. However, no sperm are transferred to the seminal vesicle. The level of polyglycylation of Ntl mutant sperm tubulin appears to be significantly lower than that of wild type controls. Glycine transporters are the most closely related SLC6 transporters to Ntl, suggesting that Ntl functions as a glycine transporter in developing sperm, where augmentation of the cytosolic pool of glycine may be required for the polyglycylation of the massive amounts of tubulin in the fly's giant sperm. The male-sterile phenotype of Ntl mutants may provide a powerful genetic system for studying the function of an SLC6 transporter family in a model organism. PMID:21298005

  16. An Automated Method for Identifying Inconsistencies within Diagrammatic Software Requirements Specifications

    NASA Technical Reports Server (NTRS)

    Zhang, Zhong

    1997-01-01

    The development of large-scale, composite software in a geographically distributed environment is an evolutionary process. Often, in such evolving systems, striving for consistency is complicated by many factors, because development participants have various locations, skills, responsibilities, roles, opinions, languages, terminology and different degrees of abstraction they employ. This naturally leads to many partial specifications or viewpoints. These multiple views on the system being developed usually overlap. From another aspect, these multiple views give rise to the potential for inconsistency. Existing CASE tools do not efficiently manage inconsistencies in distributed development environment for a large-scale project. Based on the ViewPoints framework the WHERE (Web-Based Hypertext Environment for requirements Evolution) toolkit aims to tackle inconsistency management issues within geographically distributed software development projects. Consequently, WHERE project helps make more robust software and support software assurance process. The long term goal of WHERE tools aims to the inconsistency analysis and management in requirements specifications. A framework based on Graph Grammar theory and TCMJAVA toolkit is proposed to detect inconsistencies among viewpoints. This systematic approach uses three basic operations (UNION, DIFFERENCE, INTERSECTION) to study the static behaviors of graphic and tabular notations. From these operations, subgraphs Query, Selection, Merge, Replacement operations can be derived. This approach uses graph PRODUCTIONS (rewriting rules) to study the dynamic transformations of graphs. We discuss the feasibility of implementation these operations. Also, We present the process of porting original TCM (Toolkit for Conceptual Modeling) project from C++ to Java programming language in this thesis. A scenario based on NASA International Space Station Specification is discussed to show the applicability of our approach. Finally

  17. NPRA Q and A - 1; 1990 Fuel specifications will require process changes

    SciTech Connect

    Yuan, D.C. ); Soumagne, G.; Siegrist, M.R. )

    1990-02-26

    This paper discusses the alteration of specifications for gasoline and diesel fuels in the 1990:0090s. Higher-octane gasoline with lower vapor pressure, and diesel fuel with lower sulfur and aromatics, will require process changes to produce. These altered fuel specifications prompted several questions at the most recent National Petroleum Refiners Association annual question and answer session on refining and petrochemical technology held in New Orleans, October 4-6, 1989. At this meeting, moderated by Herbert W. Bruch, NPRA's technical director, engineers and managers from refineries and petrochemical plants around the world gather to discuss their operating experiences in the form of questions and answers that cover a wide range of topics. A panel of selected technical representatives of operating companies and suppliers first answer previously submitted questions, then the audience is invited to add its contributions. This excerpt from the transcript of the meeting examines operating conditions and catalysts that increase motor octane number in FCC units, the effects on processes of lower vapor pressure gasoline specifications, and processing routes to low sulfur and aromatic diesel fuels.

  18. Sensitivity requirements of assisted BDS and GPS in specification 3GPP TS 36.171

    NASA Astrophysics Data System (ADS)

    Jin, Xiaoxi; Chen, Xin; Ying, Rendong; Yang, Genke

    2016-01-01

    With the needs of growing location-based service, a more high-performance satellite positioning technology - assisted global navigation satellite system (A-GNSS assisted-GNSS) becomes a new hotspot in area of navigation and positioning. Now, 3GPP has already provided supports for GPS, Galileo, GLONASS and QZSS, SBAS, so standardization work of introduction BDS into 3GPP organization is very imperative. In this paper, we first analysis the performance of GPS L1 C/A with assistant information, then by taking into account the difference between BDS and GPS, including the unique nature of GEO/NGEO satellites' navigation message data length and format, we design the sensitivity requirements of BDS B1 following A-GPS. The results between A-GPS and A-BDS of typical sensitivity test cases are shown in this paper, which show that the suggested sensitivity requirements satisfy the minimum performance requirements under technical specification of 3GPP TS 36.171.

  19. Topoisomerase IIβ is required for lamina-specific targeting of retinal ganglion cell axons and dendrites

    PubMed Central

    Nevin, Linda M.; Xiao, Tong; Staub, Wendy; Baier, Herwig

    2011-01-01

    The specific partnering of synaptically connected neurons is central to nervous system function. Proper wiring requires the interchange of signals between a postmitotic neuron and its environment, a distinct pattern of transcription in the nucleus, and deployment of guidance and adhesion cues to the cell surface. To identify genes involved in neurite targeting by retinal ganglion cells (GCs), their presynaptic partners in the retina, and their postsynaptic targets in the optic tectum, we undertook a forward genetic screen for mutations disrupting visual responses in zebrafish. This rapid primary screen was subsequently refined by immunohistochemical labeling of retinal and tectal neurites to detect patterning errors. From this unbiased screen, the notorious (noto) mutant exhibited the most specific phenotypes: intact retinal and tectal differentiation but multiple neurite targeting defects in the retinal inner plexiform layer (IPL) and tectal neuropil. Positional cloning and morpholino phenocopy revealed that the mutation disrupts Topoisomerase IIβ (Top2b), a broadly distributed nuclear protein involved in chromatin modifications during postmitotic differentiation. Top2b-DNA interactions are known to regulate transcription of developmentally important genes, including axon guidance factors and cell adhesion molecules, but a specific role in local synaptic targeting has not been previously described. The neurite targeting defects among GC axons are largely restricted to crossovers between sublaminae of a specific layer, SFGS, and were shown by mosaic analysis to be autonomous to the GC axons. The noto mutant provides the first example of the importance of an epigenetic regulator, Top2b, in the intricate series of events that lead to a properly wired visual system. PMID:21610027

  20. Deletion of antigen-specific immature thymocytes by dendritic cells requires LFA-1/ICAM interactions.

    PubMed

    Carlow, D A; van Oers, N S; Teh, S J; Teh, H S

    1992-03-15

    An in vitro assay was used for assessing the participation of various cell surface molecules and the efficacy of various cell types in the deletion of Ag-specific immature thymocytes. Thymocytes from mice expressing a transgenic TCR specific for the male Ag presented by the H-2Db class I MHC molecule were used as a target for deletion. In H-2d transgenic mice, cells bearing the transgenic TCR are not subjected to thymic selection as a consequence of the absence of the restricting H-2Db molecule but, nevertheless, express this TCR on the vast majority of immature CD4+8+ thymocytes. In this report we show that CD4+8+ thymocytes from H-2d TCR-transgenic mice are preferentially killed upon in vitro culture with male APC; DC were particularly effective in mediating in vitro deletion when compared with either B cells or T cells. Deletion of CD4+8+ thymocytes by DC was H-2b restricted and could be inhibited by mAb to either LFA-1 alpha or CD8. Partial inhibition was observed with mAb to ICAM-1, whereas mAb to CD4 and LFA-1 beta were without effect. These results are the first direct evidence of LFA-1 involvement in negative selection and provide further direct support for the participation of CD8/class I MHC interactions in this process. Like the requirements for deletion, activation of mature male-specific CD4-8+ T cells from female H-2b TCR-transgenic mice was also largely dependent on Ag presentation by DC and required both LFA-1/ICAM and CD8/class I MHC interactions; these results support the view that activation and deletion may represent maturation stage-dependent consequences of T cells encountering the same APC. Finally, our results also support the hypothesis that negative selection (deletion) does not require previous positive selection because deletion was observed under conditions where positive selection had not occurred.

  1. 47 CFR 95.1121 - Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 5 2013-10-01 2013-10-01 false Specific requirements for wireless medical... SERVICES Wireless Medical Telemetry Service (WMTS) General Provisions § 95.1121 Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427-1432 MHz bands. Due to the...

  2. 47 CFR 95.1121 - Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 5 2014-10-01 2014-10-01 false Specific requirements for wireless medical... SERVICES Wireless Medical Telemetry Service (WMTS) General Provisions § 95.1121 Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427-1432 MHz bands. Due to the...

  3. 47 CFR 95.1121 - Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 5 2011-10-01 2011-10-01 false Specific requirements for wireless medical... SERVICES Wireless Medical Telemetry Service (WMTS) General Provisions § 95.1121 Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427-1432 MHz bands. Due to the...

  4. 47 CFR 95.1121 - Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Specific requirements for wireless medical... SERVICES Wireless Medical Telemetry Service (WMTS) General Provisions § 95.1121 Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427-1432 MHz bands. Due to the...

  5. 47 CFR 95.1121 - Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 5 2012-10-01 2012-10-01 false Specific requirements for wireless medical... SERVICES Wireless Medical Telemetry Service (WMTS) General Provisions § 95.1121 Specific requirements for wireless medical telemetry devices operating in the 1395-1400 and 1427-1432 MHz bands. Due to the...

  6. 50 CFR Appendix F to Part 622 - Specifications for Sea Turtle Mitigation Gear and Sea Turtle Handling and Release Requirements

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Specifications for Sea Turtle Mitigation Gear and Sea Turtle Handling and Release Requirements F Appendix F to Part 622 Wildlife and Fisheries...—Specifications for Sea Turtle Mitigation Gear and Sea Turtle Handling and Release Requirements A. Sea...

  7. 50 CFR Appendix F to Part 622 - Specifications for Sea Turtle Mitigation Gear and Sea Turtle Handling and Release Requirements

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Specifications for Sea Turtle Mitigation Gear and Sea Turtle Handling and Release Requirements F Appendix F to Part 622 Wildlife and Fisheries... 622—Specifications for Sea Turtle Mitigation Gear and Sea Turtle Handling and Release Requirements...

  8. Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene Expression in Caenorhabditis

    PubMed Central

    Félix, Marie-Anne

    2016-01-01

    Patterning of C. elegans vulval cell fates relies on inductive signaling. In this induction event, a single cell, the gonadal anchor cell, secretes LIN-3/EGF and induces three out of six competent precursor cells to acquire a vulval fate. We previously showed that this developmental system is robust to a four-fold variation in lin-3/EGF genetic dose. Here using single-molecule FISH, we find that the mean level of expression of lin-3 in the anchor cell is remarkably conserved. No change in lin-3 expression level could be detected among C. elegans wild isolates and only a low level of change—less than 30%—in the Caenorhabditis genus and in Oscheius tipulae. In C. elegans, lin-3 expression in the anchor cell is known to require three transcription factor binding sites, specifically two E-boxes and a nuclear-hormone-receptor (NHR) binding site. Mutation of any of these three elements in C. elegans results in a dramatic decrease in lin-3 expression. Yet only a single E-box is found in the Drosophilae supergroup of Caenorhabditis species, including C. angaria, while the NHR-binding site likely only evolved at the base of the Elegans group. We find that a transgene from C. angaria bearing a single E-box is sufficient for normal expression in C. elegans. Even a short 58 bp cis-regulatory fragment from C. angaria with this single E-box is able to replace the three transcription factor binding sites at the endogenous C. elegans lin-3 locus, resulting in the wild-type expression level. Thus, regulatory evolution occurring in cis within a 58 bp lin-3 fragment, results in a strict requirement for the NHR binding site and a second E-box in C. elegans. This single-cell, single-molecule, quantitative and functional evo-devo study demonstrates that conserved expression levels can hide extensive change in cis-regulatory site requirements and highlights the evolution of new cis-regulatory elements required for cell-specific gene expression. PMID:27588814

  9. Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene Expression in Caenorhabditis.

    PubMed

    Barkoulas, Michalis; Vargas Velazquez, Amhed M; Peluffo, Alexandre E; Félix, Marie-Anne

    2016-09-01

    Patterning of C. elegans vulval cell fates relies on inductive signaling. In this induction event, a single cell, the gonadal anchor cell, secretes LIN-3/EGF and induces three out of six competent precursor cells to acquire a vulval fate. We previously showed that this developmental system is robust to a four-fold variation in lin-3/EGF genetic dose. Here using single-molecule FISH, we find that the mean level of expression of lin-3 in the anchor cell is remarkably conserved. No change in lin-3 expression level could be detected among C. elegans wild isolates and only a low level of change-less than 30%-in the Caenorhabditis genus and in Oscheius tipulae. In C. elegans, lin-3 expression in the anchor cell is known to require three transcription factor binding sites, specifically two E-boxes and a nuclear-hormone-receptor (NHR) binding site. Mutation of any of these three elements in C. elegans results in a dramatic decrease in lin-3 expression. Yet only a single E-box is found in the Drosophilae supergroup of Caenorhabditis species, including C. angaria, while the NHR-binding site likely only evolved at the base of the Elegans group. We find that a transgene from C. angaria bearing a single E-box is sufficient for normal expression in C. elegans. Even a short 58 bp cis-regulatory fragment from C. angaria with this single E-box is able to replace the three transcription factor binding sites at the endogenous C. elegans lin-3 locus, resulting in the wild-type expression level. Thus, regulatory evolution occurring in cis within a 58 bp lin-3 fragment, results in a strict requirement for the NHR binding site and a second E-box in C. elegans. This single-cell, single-molecule, quantitative and functional evo-devo study demonstrates that conserved expression levels can hide extensive change in cis-regulatory site requirements and highlights the evolution of new cis-regulatory elements required for cell-specific gene expression. PMID:27588814

  10. SU-E-T-72: Commissioning of a Standardized SRS Cone Set: Determination of the Bolus Gap Factors in a Passively Scattered Proton Beam

    SciTech Connect

    Simpson, R; Gordon, I; Ghebremedhin, A; Wroe, A; Schulte, R; Bush, D; Slater, J; Patyal, B

    2014-06-01

    Purpose: To determine the proton output factors for an SRS cone set using standardized apertures and varied range compensators (bolus blanks); specifically, to determine the best method for modeling the bolus gap factor (BGF) and eliminate the need for patient specific calibrations. Methods: A Standard Imaging A-16 chamber was placed in a Plastic Water phantom to measure the change in dose/MU with different treatment combinations for a proton SRS cone, using standardized apertures and range compensators. Measurements were made with all apertures in the SRS cone set, with four different range compensator thicknesses and five different air gaps between the end of the SRS cone and the surface of the phantom. The chamber was located at iso-center and maintained at a constant depth at the center of modulation for all measurements. Each aperture was placed in the cone to measure the change in MU needed to maintain constant dose at the chamber, as the air gap was increased with different thicknesses of bolus. Results: The dose/MU varied significantly with decreasing aperture size, increasing bolus thickness, or increasing air gap. The measured data was fitted with the lowest order polynomials that accurately described the data, to create a model for determining the change in output for any potential combination of devices used to treat a patient. For a given standardized aperture, the BGF could be described by its constituent factors: the bolus thickness factor (BTF) and the nozzle extension factor (NEF). Conclusion: The methods used to model the dose at the calibration point could be used to accurately predict the change in output for SRS proton beams due to the BGF, eliminating the need for patient specific calibrations. This method for modeling SRS treatments could also be applied to model other treatments using passively scattered proton beams.

  11. The RED domain of Paired is specifically required for Drosophila accessory gland maturation

    PubMed Central

    Li, Li; Li, Ping; Xue, Lei

    2015-01-01

    The evolutionarily conserved paired domain consists of the N-terminal PAI and the C-terminal RED domains, each containing a helix–turn–helix motif capable of binding DNA. Despite its conserved sequence, the physiological functions of the RED domain remain elusive. Here, we constructed a prd transgene expressing a truncated Paired (Prd) protein without the RED domain, and examined its rescue ability in prd mutants. We found that the RED domain is specifically required for the expression of Acp26Aa and sex peptide in male accessory glands, and the induction of female post-mating response. Our data thus identified an important physiological function for the evolutionarily conserved RED domain. PMID:25694546

  12. The RED domain of Paired is specifically required for Drosophila accessory gland maturation.

    PubMed

    Li, Li; Li, Ping; Xue, Lei

    2015-02-01

    The evolutionarily conserved paired domain consists of the N-terminal PAI and the C-terminal RED domains, each containing a helix-turn-helix motif capable of binding DNA. Despite its conserved sequence, the physiological functions of the RED domain remain elusive. Here, we constructed a prd transgene expressing a truncated Paired (Prd) protein without the RED domain, and examined its rescue ability in prd mutants. We found that the RED domain is specifically required for the expression of Acp26Aa and sex peptide in male accessory glands, and the induction of female post-mating response. Our data thus identified an important physiological function for the evolutionarily conserved RED domain.

  13. The Specific Requirements for CR1 Retrotransposition Explain the Scarcity of Retrogenes in Birds.

    PubMed

    Suh, Alexander

    2015-08-01

    Chicken repeat 1 (CR1) retroposons are the most abundant superfamily of transposable elements in the genomes of birds, crocodilians, and turtles. However, CR1 mobilization remains poorly understood. In this article, I document that the diverse CR1 lineages of land vertebrates share a highly conserved hairpin structure and an octamer microsatellite motif at their very 3' ends. Together with the presence of these same motifs in the tails of CR1-mobilized short interspersed elements, this suggests that the minimum requirement for CR1 transcript recognition and retrotransposition is a complex >50-nt structure. Such a highly specific recognition sequence readily explains why CR1-dominated genomes generally contain very few retrogenes. Conversely, the mammalian richness in retrogenes results from CR1 extinction in their early evolution and subsequent establishment of L1 dominance. PMID:26223967

  14. Removal of damaged proteins during ES cell fate specification requires the proteasome activator PA28

    PubMed Central

    Hernebring, Malin; Fredriksson, Åsa; Liljevald, Maria; Cvijovic, Marija; Norrman, Karin; Wiseman, John; Semb, Henrik; Nyström, Thomas

    2013-01-01

    In embryonic stem cells, removal of oxidatively damaged proteins is triggered upon the first signs of cell fate specification but the underlying mechanism is not known. Here, we report that this phase of differentiation encompasses an unexpected induction of genes encoding the proteasome activator PA28αβ (11S), subunits of the immunoproteasome (20Si), and the 20Si regulator TNFα. This induction is accompanied by assembly of mature PA28-20S(i) proteasomes and elevated proteasome activity. Inhibiting accumulation of PA28α using miRNA counteracted the removal of damaged proteins demonstrating that PA28αβ has a hitherto unidentified role required for resetting the levels of protein damage at the transition from self-renewal to cell differentiation. PMID:23459332

  15. Rapid amyloid fibril formation by a winter flounder antifreeze protein requires specific interaction with ice.

    PubMed

    Dubé, André; Leggiadro, Cindy; Ewart, Kathryn Vanya

    2016-05-01

    A typically α-helical antifreeze protein (wflAFP-6) from winter flounder, Pseudopleuronectes americanus, forms amyloid fibrils during freezing. In this study, the effects of distinct components of the freezing process were examined. Freezing of wflAFP-6 in the presence of template ice was shown to be necessary for rapid conversion to an amyloid conformation. Neither subfreezing temperature nor phase change was sufficient. Thus, specific interaction with the ice surface was essential. The ice-induced formation of amyloid appeared to be unique to this helical antifreeze, it required high concentrations of protein and it occurred over a range of pH values. These results define a method for rapid formation of amyloid by wflAFP-6 on demand under physiological conditions. PMID:27086686

  16. Category-specific organization in the human brain does not require visual experience.

    PubMed

    Mahon, Bradford Z; Anzellotti, Stefano; Schwarzbach, Jens; Zampini, Massimiliano; Caramazza, Alfonso

    2009-08-13

    Distinct regions within the ventral visual pathway show neural specialization for nonliving and living stimuli (e.g., tools, houses versus animals, faces). The causes of these category preferences are widely debated. Using functional magnetic resonance imaging, we find that the same regions of the ventral stream that show category preferences for nonliving stimuli and animals in sighted adults show the same category preferences in adults who are blind since birth. Both blind and sighted participants had larger blood oxygen-level dependent (BOLD) responses in the medial fusiform gyrus for nonliving stimuli compared to animal stimuli and differential BOLD responses in lateral occipital cortex for animal stimuli compared to nonliving stimuli. These findings demonstrate that the medial-to-lateral bias by conceptual domain in the ventral visual pathway does not require visual experience in order to develop and suggest the operation of innately determined domain-specific constraints on the organization of object knowledge. PMID:19679078

  17. Phenotype classification of single cells using SRS microscopy, RNA sequencing, and microfluidics (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Streets, Aaron M.; Cao, Chen; Zhang, Xiannian; Huang, Yanyi

    2016-03-01

    Phenotype classification of single cells reveals biological variation that is masked in ensemble measurement. This heterogeneity is found in gene and protein expression as well as in cell morphology. Many techniques are available to probe phenotypic heterogeneity at the single cell level, for example quantitative imaging and single-cell RNA sequencing, but it is difficult to perform multiple assays on the same single cell. In order to directly track correlation between morphology and gene expression at the single cell level, we developed a microfluidic platform for quantitative coherent Raman imaging and immediate RNA sequencing (RNA-Seq) of single cells. With this device we actively sort and trap cells for analysis with stimulated Raman scattering microscopy (SRS). The cells are then processed in parallel pipelines for lysis, and preparation of cDNA for high-throughput transcriptome sequencing. SRS microscopy offers three-dimensional imaging with chemical specificity for quantitative analysis of protein and lipid distribution in single cells. Meanwhile, the microfluidic platform facilitates single-cell manipulation, minimizes contamination, and furthermore, provides improved RNA-Seq detection sensitivity and measurement precision, which is necessary for differentiating biological variability from technical noise. By combining coherent Raman microscopy with RNA sequencing, we can better understand the relationship between cellular morphology and gene expression at the single-cell level.

  18. Performance Modeling Applied to the Treatment and Disposal of a Mixed Waste at the SRS

    SciTech Connect

    Pickett, J.B.; Jantzen, C.M.; Cook, J.R.; Whited, A.R.; Field, R.A.

    1997-05-01

    Performance modeling for Low Level Mixed Waste disposal was conducted using the measured leach rates from a number of vitrified waste formulations. The objective of the study was to determine if the improved durability of a vitrified mixed waste would allow trench disposal at the Savannah River Site (SRS). Leaching data were compiled from twenty-nine diverse reference glasses, encompassing a wide range of exposed glass surface area to leachant volume ratios (SA/V), and various leachant solutions; all of which had been leached at 90 degrees Celsius, using the MCC-1 or PCT procedures (ASTM Procedures C1220-92 and C1285-94, respectively). The normalized leach rates were scaled to the ambient disposal temperature of 25 degrees Celsius, and compared to the allowable leach rate of uranium - which would meet the performance assessment requirements. The results indicated that a glass of above average durability (vs. the reference glasses) would meet the uranium leaching concentration for direct SRS trench disposal.

  19. Rapid Separation Methods to Characterize Actinides and Metallic Impurities in Plutonium Scrap Materials at SRS

    SciTech Connect

    Maxwell, S.L. III; Jones, V.D.

    1998-07-01

    The Nuclear Materials Stabilization and Storage Division at SRS plans to stabilize selected plutonium scrap residue materials for long term storage by dissolution processing and plans to stabilize other plutonium vault materials via high-temperature furnace processing. To support these nuclear material stabilization activities, the SRS Analytical Laboratories Department (ALD) will provide characterization of materials required prior to the dissolution or the high-firing of these materials. Lab renovations to install new analytical instrumentation are underway to support these activities that include glove boxes with simulated-process dissolution and high- pressure microwave dissolution capability. Inductively-coupled plasma atomic emission spectrometry (ICP-AES), inductively- coupled mass spectrometry (ICP-MS) and thermal-ionization mass spectrometry (TIMS) will be used to measure actinide isotopics and metallic impurities. New high-speed actinide separation methods have been developed that will be applied to isotopic characterization of nuclear materials by TIMS and ICP-MS to eliminate isobaric interferences between Pu-238 /U- 238 and Pu-241/Am-241. TEVA Resin, UTEVA Resin, and TRU Resin columns will be used with vacuum-assisted flow rates to minimize TIMS and ICP-MS sample turnaround times. For metallic impurity analysis, rapid column removal methods using UTEVA Resin, AGMP-1 anion resin and AG MP-50 cation resin have also been developed to remove plutonium and uranium matrix interferences prior to ICP-AES and ICP- MS measurements.

  20. Environmental Molecular Sciences Laboratory Operations System: Version 4.0 - system requirements specification

    SciTech Connect

    Kashporenko, D.

    1996-07-01

    This document is intended to provide an operations standard for the Environmental Molecular Sciences Laboratory OPerations System (EMSL OPS). It is directed toward three primary audiences: (1) Environmental Molecular Sciences Laboratory (EMSL) facility and operations personnel; (2) laboratory line managers and staff; and (3) researchers, equipment operators, and laboratory users. It is also a statement of system requirements for software developers of EMSL OPS. The need for a finely tuned, superior research environment as provided by the US Department of Energy`s (DOE) Environmental Molecular Sciences Laboratory has never been greater. The abrupt end of the Cold War and the realignment of national priorities caused major US and competing overseas laboratories to reposition themselves in a highly competitive research marketplace. For a new laboratory such as the EMSL, this means coming into existence in a rapidly changing external environment. For any major laboratory, these changes create funding uncertainties and increasing global competition along with concomitant demands for higher standards of research product quality and innovation. While more laboratories are chasing fewer funding dollars, research ideas and proposals, especially for molecular-level research in the materials and biological sciences, are burgeoning. In such an economically constrained atmosphere, reduced costs, improved productivity, and strategic research project portfolio building become essential to establish and maintain any distinct competitive advantage. For EMSL, this environment and these demands require clear operational objectives, specific goals, and a well-crafted strategy. Specific goals will evolve and change with the evolution of the nature and definition of DOE`s environmental research needs. Hence, EMSL OPS is designed to facilitate migration of these changes with ease into every pertinent job function, creating a facile {open_quotes}learning organization.{close_quotes}

  1. Sex specific retinoic acid signaling is required for the initiation of urogenital sinus bud development.

    PubMed

    Bryant, Sarah L; Francis, Jeffrey C; Lokody, Isabel B; Wang, Hong; Risbridger, Gail P; Loveland, Kate L; Swain, Amanda

    2014-11-15

    The mammalian urogenital sinus (UGS) develops in a sex specific manner, giving rise to the prostate in the male and the sinus vagina in the embryonic female. Androgens, produced by the embryonic testis, have been shown to be crucial to this process. In this study we show that retinoic acid signaling is required for the initial stages of bud development from the male UGS. Enzymes involved in retinoic acid synthesis are expressed in the UGS mesenchyme in a sex specific manner and addition of ligand to female tissue is able to induce prostate-like bud formation in the absence of androgens, albeit at reduced potency. Functional studies in mouse organ cultures that faithfully reproduce the initiation of prostate development indicate that one of the roles of retinoic acid signaling in the male is to inhibit the expression of Inhba, which encodes the βA subunit of Activin, in the UGS mesenchyme. Through in vivo genetic analysis and culture studies we show that inhibition of Activin signaling in the female UGS leads to a similar phenotype to that of retinoic acid treatment, namely bud formation in the absence of androgens. Our data also reveals that both androgens and retinoic acid have extra independent roles to that of repressing Activin signaling in the development of the prostate during fetal stages. This study identifies a novel role for retinoic acid as a mesenchymal factor that acts together with androgens to determine the position and initiation of bud development in the male UGS epithelia. PMID:25261715

  2. A Symmetrical, Planar SOFC Design for NASA's High Specific Power Density Requirements

    NASA Technical Reports Server (NTRS)

    Cable, Thomas L.; Sofie, Stephen W.

    2007-01-01

    Solid oxide fuel cell (SOFC) systems for aircraft applications require an order of magnitude increase in specific power density (1.0 kW/kg) and long life. While significant research is underway to develop anode supported cells which operate at temperatures in the range of 650-800 C, concerns about Cr-contamination from the metal interconnect may drive the operating temperature down further, to 750 C and lower. Higher temperatures, 900-1000 C, are more favorable for SOFC stacks to achieve specific power densities of 1.0 kW/kg. Since metal interconnects are not practical at these high temperatures and can account for up to 75% of the weight of the stack, NASA is pursuing a design that uses a thin, LaCrO3-based ceramic interconnect that incorporates gas channels into the electrodes. The bi-electrode supported cell (BSC) uses porous YSZ scaffolds, on either side of a 10-20 microns electrolyte. The porous support regions are fabricated with graded porosity using the freeze-tape casting process which can be tailored for fuel and air flow. Removing gas channels from the interconnect simplifies the stack design and allows the ceramic interconnect to be kept thin, on the order of 50 -100 microns. The YSZ electrode scaffolds are infiltrated with active electrode materials following the high temperature sintering step. The NASA-BSC is symmetrical and CTE matched, providing balanced stresses and favorable mechanical properties for vibration and thermal cycling.

  3. Root hair-specific EXPANSIN A7 is required for root hair elongation in Arabidopsis.

    PubMed

    Lin, Changfa; Choi, Hee-Seung; Cho, Hyung-Taeg

    2011-04-01

    Expansins are non-hydrolytic cell wall-loosening proteins that are involved in the cell wall modifications that underlie many plant developmental processes. Root hair growth requires the accumulation of cell wall materials and dynamic cell wall modification at the tip region. Although several lines of indirect evidence support the idea that expansin-mediated wall modification occurs during root hair growth, the involvement of these proteins remains to be demonstrated in vivo. In this study, we used RNA interference (RNAi) to examine the biological function of Arabidopsis thaliana EXPANSIN A7 (AtEXPA7), which is expressed specifically in the root hair cell. The root hairspecific AtEXPA7 promoter was used to drive RNAi expression, which targeted two independent regions in the AtEXPA7 transcript. Quantitative reverse transcriptase-PCR analyses were used to examine AtEXPA7 transcript levels. In four independent RNAi transformant lines, RNAi expression reduced AtEXPA7 transcript levels by 25-58% compared to controls. Accordingly, the root hairs of RNAi transformant lines were 25-48% shorter than control plants and exhibited a broader range of lengths than the controls. Our results provide in vivo evidence that expansins are required for root hair tip growth.

  4. Zic2 is an enhancer-binding factor required for embryonic stem cell specification

    PubMed Central

    Luo, Zhuojuan; Gao, Xin; Lin, Chengqi; Smith, Edwin; Marshall, Stacy; Swanson, Selene K.; Florens, Laurence; Washburn, Michael P.; Shilatifard, Ali

    2016-01-01

    SUMMARY The Zinc finger protein of the cerebellum 2 (Zic2) is one of the vertebrate homologs of the Drosophila pair-rule gene odd-paired (opa). Our molecular and biochemical studies demonstrate that Zic2 preferentially binds to transcriptional enhancers and is required for the regulation of gene expression in embryonic stem cells. Detailed genome-wide and molecular studies reveal that Zic2 can function with Mbd3/NuRD in regulating the chromatin state and transcriptional output of genes linked to differentiation. Zic2 is required for proper differentiation of ES cells, similar to what has been previously reported for Mbd3/NuRD. Our study identifies Zic2 as a key factor in the execution of transcriptional fine-tuning with Mbd3/NuRD in ES cells through interactions with enhancers. Our study also points to the role of the Zic family of proteins as enhancer-specific binding factors functioning in development. PMID:25699711

  5. Specific factors are required for kinase-dependent endocytosis of insulin receptors.

    PubMed Central

    Welsh, J B; Worthylake, R; Wiley, H S; Gill, G N

    1994-01-01

    Mouse B82 cells that support high affinity saturable endocytosis of epidermal growth factor receptors (EGFR) exhibited only low rates of nonsaturable internalization of insulin receptors (InsR). To investigate the defect in endocytosis of InsR in B82 cells, we examined the role of sequence motifs and tyrosine kinase, the two receptor components shown to be required for efficient saturable endocytosis of InsR in Rat 1 cells. Placement of residues encoded by exon 16 of the InsR onto an EGFR truncated to residue 958 restored EGF-induced internalization of this mutant receptor indicating that the sequence codes in exon 16 are recognized by B82 cells. To determine whether the kinase function could be provided in trans, a B82 cell expressing both receptors was established. EGF-activated EGFR kinase was not able to restore insulin-dependent rapid endocytosis to InsR. However, fusion of untransfected Rat1 cells with InsR-expressing B82 cells enabled rapid endocytosis of InsR, indicating that the internalization defect can be complemented. These results indicate that, although internalization codes can function in the context of other receptors, activation of tyrosine kinase receptors requires an additional specific component. Images PMID:7919536

  6. Pdx-1 is required for activation in vivo from a duodenum-specific enhancer.

    PubMed

    Dusing, M R; Florence, E A; Wiginton, D A

    2001-04-27

    The purine metabolic gene adenosine deaminase (ADA) is expressed along a defined spatiotemporal pattern in the developing mammalian small intestine, where high-level expression is limited to the villous epithelium of the duodenum. This activation is observed in rodents as the intestine completes the final maturation resulting in adult crypt-villus structures at 2-3 weeks postpartum. A regulatory module responsible for this pattern of expression has been identified in the second intron of the human ADA gene. Of the multiple duodenal proteins that can interact with this small duodenal enhancer region, the studies contained in this work describe the identification of five of these proteins as the dispersed homeobox protein PDX-1. This transcription factor exhibits a profile of expression in the small intestine similar to that observed for ADA, making it an ideal candidate factor for the duodenum-specific ADA enhancer. Loss of PDX-1 binding, via a PDX-1 mutated enhancer transgenic construction, resulted in complete loss of high-level activation in the duodenum, demonstrating the absolute requirement for this factor in vivo. However, co-transfection experiments suggest that other proteins that bind the enhancer are also required for enhancer function because PDX-1 alone was incapable of significant transactivation.

  7. Specific Requirements of Physiotherapists on the Practical Use of Software in the Therapeutical Process.

    PubMed

    Messer-Misak, Karin; Egger, Rudolf

    2016-01-01

    The current healthcare system requires more effective management. New media and technology are supposed to support the demands of the current healthcare system. By the example of physiotherapy, the primary objective of this study was to define the specific requirements of therapists on the practical use of software which cover the administration, documentation and evaluation of the entire therapy process, including a database with pictures/videos about exercises which can be adapted individually by the therapists. Another objective was to show what conditions for a successful implementation of advanced applications during the entire treatment process have to be fulfilled. The approach of mixed-methods designs was chosen. In the first part a two-stage qualitative study was carried out, followed by a quantitative survey. The results show that the use of the software regarding the therapy-related part is dependent on how adaptable the software is to the special needs of the therapists, that the whole treatment process is mapped on the software and that an additional training during the professional practice must be implemented in order to deploy the use of the software successfully in the therapeutic process. PMID:27139399

  8. Engineering Specification for Large-aperture UVO Space Telescopes Derived from Science Requirements

    NASA Technical Reports Server (NTRS)

    Stahl, H. Philip; Postman, Marc; Smith, W. Scott

    2013-01-01

    The Advance Mirror Technology Development (AMTD) project is a three year effort initiated in FY12 to mature by at least a half TRL step six critical technologies required to enable 4 to 8 meter UVOIR space telescope primary mirror assemblies for both general astrophysics and ultra-high contrast observations of exoplanets. AMTD uses a science-driven systems engineering approach. We mature technologies required to enable the highest priority science AND result in a high-performance low-cost low-risk system. To provide the science community with options, we are pursuing multiple technology paths. We have assembled an outstanding team from academia, industry, and government with extensive expertise in astrophysics and exoplanet characterization, and in the design/manufacture of monolithic and segmented space telescopes. A key accomplishment is deriving engineering specifications for advanced normal-incidence monolithic and segmented mirror systems needed to enable both general astrophysics and ultra-high contrast observations of exoplanets missions as a function of potential launch vehicles and their mass and volume constraints.

  9. Differential requirements for Gli2 and Gli3 in the regional specification of the mouse hypothalamus

    PubMed Central

    Haddad-Tóvolli, Roberta; Paul, Fabian A.; Zhang, Yuanfeng; Zhou, Xunlei; Theil, Thomas; Puelles, Luis; Blaess, Sandra; Alvarez-Bolado, Gonzalo

    2015-01-01

    Secreted protein Sonic hedgehog (Shh) ventralizes the neural tube by modulating the crucial balance between activating and repressing functions (GliA, GliR) of transcription factors Gli2 and Gli3. This balance—the Shh-Gli code—is species- and context-dependent and has been elucidated for the mouse spinal cord. The hypothalamus, a forebrain region regulating vital functions like homeostasis and hormone secretion, shows dynamic and intricate Shh expression as well as complex regional differentiation. Here we asked if particular combinations of Gli2 and Gli3 and of GliA and GliR functions contribute to the variety of hypothalamic regions, i.e., we wanted to approach the question of a possible hypothalamic version of the Shh-Gli code. Based on mouse mutant analysis, we show that: (1) hypothalamic regional heterogeneity is based in part on differentially stringent requirements for Gli2 or Gli3; (2) another source of diversity are differential requirements for Shh of neural vs. non-neural origin; (3) the medial progenitor domain known to depend on Gli2 for its development generates several essential hypothalamic nuclei plus the pituitary and median eminence; (4) the suppression of Gli3R by neural and non-neural Shh is essential for hypothalamic specification. Finally, we have mapped our results on a recent model which considers the hypothalamus as a transverse region with alar and basal portions. Our data confirm the model and are explained by it. PMID:25859185

  10. 10 CFR 33.15 - Requirements for the issuance of a Type C specific license of broad scope.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Requirements for the issuance of a Type C specific license... issuance of a Type C specific license of broad scope. An application for a Type C specific license of broad... bachelor level, or equivalent training and experience, in the physical or biological sciences or...

  11. Functional requirements analysis and human machine interface specifications for handheld metal detector wands

    SciTech Connect

    Hoang, V.; Hartney, C.; Banks, W.

    1994-11-01

    Functional Requirements Analysis (FRA) and Human-Machine-Interface Design Specifications (HMIDs) are critical elements in the development of effective security systems. Handheld metal detector wands are currently used by security personnel to detect metal weapons and munitions that might be smuggled onboard an aircraft by terrorists or individuals who intend to do harm to passengers, aircraft, or other air carrier-related targets. The FAA has requested that Lawrence Livermore National Laboratory (LLNL) assist in developing functional requirements for handheld metal detector devices (wands) used at airports. This effort is focused on both defining and assuring adequate functional and human interface designs that are an integral part of airport security operations. In addition to developing functional requirements, LLNL was also requested to examine and review wanding procedures currently used by the airports and air carriers and provide comments, recommendations, and suggestions for enhanced security based upon this review. The phrase ``Human-Machine-Interface`` (HMI) is frequently used to describe the characteristics of a system that allows the human to interact and control the machine or system. Equipment used by checkpoint security Pre-Board Screeners (PBS`s) during rapid search of passengers must be designed to fit a broad range of anthropometric differences in height, hand size, grip strength, upper body strength, visual. acuity, auditory acuity, and other related human variables. In essence, if there is a high degree of compatibility between the end-user and the equipment, there will be a direct enhancement of total system performance and system operability. Thus, this document may also be used as, a guideline to enhance ergonomic compatibility between the PBS`s and the equipment they use.

  12. 40 CFR 180.1301 - Escherichia coli O157:H7 specific bacteriophages; temporary exemption from the requirement of a...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... bacteriophages; temporary exemption from the requirement of a tolerance. 180.1301 Section 180.1301 Protection of... bacteriophages; temporary exemption from the requirement of a tolerance. A temporary exemption from the requirement of a tolerance is established for residues of lytic bacteriophages that are specific...

  13. Vitrification of actinide solutions in SRS separations facilities

    SciTech Connect

    Minichan, R.L.; Ramsey, W.G.

    1995-09-01

    The actinide vitrification system being developed at SRS provides the capability to convert specialized or unique forms of nuclear material into a stable solid glass product that can be safely shipped, stored or reprocessed according to the DOE complex mission. This project is an application of technology developed through funds from the Office of Technology Development (OTD). This technology is ideally suited for vitrifying relatively small quantities of fissile or special nuclear material since it is designed to be critically safe. Successful demonstration of this system to safely vitrify radioactive material could open up numerous opportunities for transferring this technology to applications throughout the DOE complex.

  14. Lead pyrovanadate single crystal as a new SRS material

    SciTech Connect

    Basiev, Tasoltan T; Voronko, Yu K; Maslov, Vladislav A; Sobol, A A; Shukshin, V E

    2011-02-28

    Lead pyrovanadate Pb{sub 2}V{sub 2}O{sub 7} single crystals of optical quality suitable for laser experiments are obtained. Vibrational modes are identified based on the analysis of the polarised Raman spectra of the single crystals. The main parameters (width at half maximum, peak and integral intensities) of the spectral lines most promising for SRS conversion in this material are estimated. These parameters are compared with the corresponding parameters of the most frequently used lines of known Raman materials: yttrium and gadolinium vanadates, potassium and lead tungstates, and lead molybdate. (active media)

  15. SRS facility impacts on Crackerneck Wildlife Management Area

    SciTech Connect

    Blanchard, A.

    2000-01-11

    Savannah River site (SRS) facilities that contain hazardous materials have completed the Emergency Preparedness Hazards Assessment (EPHA) process in accordance with Emergency Management Program Procedure (EMPP) 6Q-001. The EPHA determines the consequences of releases from these facilities and identifies events that exceed Protective Action Criteria (PAC) at defined receptor locations for areas of interest. One such area of interest is the Crackerneck wildlife Management Area (WMA). As such, facilities with releases that have the potential to exceed PAC at the Crackerneck WMA have been identified.

  16. Systems, methods and apparatus for implementation of formal specifications derived from informal requirements

    NASA Technical Reports Server (NTRS)

    Hinchey, Michael G. (Inventor); Rash, James L. (Inventor); Erickson, John D. (Inventor); Gracinin, Denis (Inventor); Rouff, Christopher A. (Inventor)

    2010-01-01

    Systems, methods and apparatus are provided through which in some embodiments an informal specification is translated without human intervention into a formal specification. In some embodiments the formal specification is a process-based specification. In some embodiments, the formal specification is translated into a high-level computer programming language which is further compiled into a set of executable computer instructions.

  17. Enterprise SRS: leveraging ongoing operations to advance nuclear fuel cycles research and development programs

    SciTech Connect

    Murray, A.M.; Marra, J.E.; Wilmarth, W.R.; McGuire, P.W.; Wheeler, V.B.

    2013-07-01

    The Savannah River Site (SRS) is re-purposing its vast array of assets (including H Canyon - a nuclear chemical separation plant) to solve issues regarding advanced nuclear fuel cycle technologies, nuclear materials processing, packaging, storage and disposition. The vehicle for this transformation is Enterprise SRS which presents a new, radical view of SRS as a united endeavor for 'all things nuclear' as opposed to a group of distinct and separate entities with individual missions and organizations. Key among the Enterprise SRS strategic initiatives is the integration of research into SRS facilities but also in other facilities in conjunction with on-going missions to provide researchers from other national laboratories, academic institutions, and commercial entities the opportunity to demonstrate their technologies in a relevant environment and scale prior to deployment. To manage that integration of research demonstrations into site facilities, a center for applied nuclear materials processing and engineering research has been established in SRS.

  18. SRS MOX fuel lead assemblies data report for the surplus plutonium disposition environmental impact statement

    SciTech Connect

    O`Connor, D.G.; Fisher, S.E.; Holdaway, R.

    1998-08-01

    The purpose of this document is to support the US Department of Energy (DOE) Fissile Materials Disposition Program`s preparation of the draft surplus plutonium disposition environmental impact statement. This is one of several responses to data call requests for background information on activities associated with the operation of the lead assembly (LA) mixed-oxide (MOX) fuel fabrication facility. DOE-MD requested that the DOE Site Operations Offices nominate DOE sites that meet established minimum requirements that could produce MOX LAs. Six initial site combinations were proposed: (1) Argonne National Laboratory-West (ANL-W) with support from Idaho National Engineering and Environmental Laboratory (INEEL), (2) Hanford, (3) Los Alamos National Laboratory (LANL) with support from Pantex, (4) Lawrence Livermore National Laboratory (LLNL), (5) Oak Ridge Reservation (ORR), and (6) Savannah River Site(SRS). After further analysis by the sites and DOE-MD, five site combinations were established as possible candidates for producing MOX LAs: (1) ANL-W with support from INEEL, (2) Hanford, (3) LANL, (4) LLNL, and (5) SRS. SRS has proposed an LA MOX fuel fabrication approach that would be done entirely inside an S and S Category 1 area. An alternate approach would allow fabrication of fuel pellets and assembly of fuel rods in an S and S Category 2 or 3 facility with storage of bulk PuO{sub 2} and assembly, storage, and shipping of fuel bundles in an S and S Category 1 facility. The total Category 1 approach, which is the recommended option, would be done in the 221-H Canyon Building. A facility that was never in service will be removed from one area, and a hardened wall will be constructed in another area to accommodate execution of the LA fuel fabrication. The non-Category 1 approach would require removal of process equipment in the FB-Line metal production and packaging glove boxes, which requires work in a contamination area. The Immobilization Hot Demonstration Program

  19. Phagocytosis of virulent Porphyromonas gingivalis by human polymorphonuclear leukocytes requires specific immunoglobulin G.

    PubMed Central

    Cutler, C W; Kalmar, J R; Arnold, R R

    1991-01-01

    No studies to date clearly define the interactions between Porphyromonas gingivalis and human peripheral blood polymorphonuclear leukocytes (PMN), nor has a protective role for antibody to P. gingivalis been defined. Using a fluorochrome phagocytosis microassay, we investigated PMN phagocytosis and killing of P. gingivalis as a function of P. gingivalis-specific antibody. Sera from a nonimmune rabbit and a healthy human subject were not opsonic for virulent P. gingivalis A7436, W83, and HG405; phagocytosis of these strains (but not 33277) required opsonization with hyperimmune antiserum (RaPg). Diluting RaPg with a constant complement source decreased proportionally the number of P. gingivalis A7436 cells phagocytosed per phagocytic PMN. Enriching for the immunoglobulin G fraction of RAPg A7436 enriched for opsonic activity toward A7436. An opsonic evaluation of 18 serum samples from adult periodontitis patients revealed that only 3 adult periodontitis sera of 17 with elevated immunoglobulin G to P. gingivalis A7436 were opsonic for A7436 and, moreover, that the serum sample with the highest enzyme-linked immunosorbent assay titer was most opsonic (patient 1). However, the opsonic activity of serum from patient 1 was qualitatively and not just quantitatively different from that of the nonopsonic human sera (but was less effective opsonin than RaPg). Strain variability was observed in resistance of P. gingivalis to phagocytosis, and opsonization was strain specific for some, but not all, strains tested. An evaluation of killing of A7436 revealed that serum killing and extracellular killing of P. gingivalis were less effective alone when compared with intracellular PMN killing alone. PMID:2037370

  20. The rapidly evolving centromere-specific histone has stringent functional requirements in Arabidopsis thaliana.

    PubMed

    Ravi, Maruthachalam; Kwong, Pak N; Menorca, Ron M G; Valencia, Joel T; Ramahi, Joseph S; Stewart, Jodi L; Tran, Robert K; Sundaresan, Venkatesan; Comai, Luca; Chan, Simon W-L

    2010-10-01

    Centromeres control chromosome inheritance in eukaryotes, yet their DNA structure and primary sequence are hypervariable. Most animals and plants have megabases of tandem repeats at their centromeres, unlike yeast with unique centromere sequences. Centromere function requires the centromere-specific histone CENH3 (CENP-A in human), which replaces histone H3 in centromeric nucleosomes. CENH3 evolves rapidly, particularly in its N-terminal tail domain. A portion of the CENH3 histone-fold domain, the CENP-A targeting domain (CATD), has been previously shown to confer kinetochore localization and centromere function when swapped into human H3. Furthermore, CENP-A in human cells can be functionally replaced by CENH3 from distantly related organisms including Saccharomyces cerevisiae. We have used cenh3-1 (a null mutant in Arabidopsis thaliana) to replace endogenous CENH3 with GFP-tagged variants. A H3.3 tail domain-CENH3 histone-fold domain chimera rescued viability of cenh3-1, but CENH3's lacking a tail domain were nonfunctional. In contrast to human results, H3 containing the A. thaliana CATD cannot complement cenh3-1. GFP-CENH3 from the sister species A. arenosa functionally replaces A. thaliana CENH3. GFP-CENH3 from the close relative Brassica rapa was targeted to centromeres, but did not complement cenh3-1, indicating that kinetochore localization and centromere function can be uncoupled. We conclude that CENH3 function in A. thaliana, an organism with large tandem repeat centromeres, has stringent requirements for functional complementation in mitosis.

  1. The regulation of glial-specific splicing of Neurexin IV requires HOW and Cdk12 activity.

    PubMed

    Rodrigues, Floriano; Thuma, Leila; Klämbt, Christian

    2012-05-01

    The differentiation of the blood-brain barrier (BBB) is an essential process in the development of a complex nervous system and depends on alternative splicing. In the fly BBB, glial cells establish intensive septate junctions that require the cell-adhesion molecule Neurexin IV. Alternative splicing generates two different Neurexin IV isoforms: Neurexin IV(exon3), which is found in cells that form septate junctions, and Neurexin IV(exon4), which is found in neurons that form no septate junctions. Here, we show that the formation of the BBB depends on the RNA-binding protein HOW (Held out wings), which triggers glial specific splicing of Neurexin IV(exon3). Using a set of splice reporters, we show that one HOW-binding site is needed to include one of the two mutually exclusive exons 3 and 4, whereas binding at the three further motifs is needed to exclude exon 4. The differential splicing is controlled by nuclear access of HOW and can be induced in neurons following expression of nuclear HOW. Using a novel in vivo two-color splicing detector, we then screened for genes required for full HOW activity. This approach identified Cyclin-dependent kinase 12 (Cdk12) and the splicesosomal component Prp40 as major determinants in regulating HOW-dependent splicing of Neurexin IV. Thus, in addition to the control of nuclear localization of HOW, the phosphorylation of the C-terminal domain of the RNA polymerase II by Cdk12 provides an elegant mechanism in regulating timed splicing of newly synthesized mRNA molecules.

  2. Conversion of the SRS to a higher brilliance lattice

    SciTech Connect

    Thompson, D. J.; Suller, V. P.

    1989-07-01

    The SRS at Daresbury has been upgraded by the addition of quadrupoles which reduce the emittance by a factor of 15. In order to do this without disturbing the beamlines, all 16 straight sections were rebuilt. Additional sextupole and octupole magnets were included, as was additional pumping capacity. Completely new beam monitor and vertical steering systems have been incorporated. The SRS was shut down on 1 October 1986 and, after recommissioning, regular scheduled operation for users at the rate of nearly 6000 h per year has been in effect again since June 1987. Scheduled operation is at 2 GeV with the 5T wiggler operational. The gain in brightness is as expected. Maximum beam current at 2 GeV is now 280 mA. Beam lifetime is 20 h at 200 mA. Diffraction patterns of small angle scattering from muscle obtained with an electronic area detector show that with the improved brilliance, diffraction lines corresponding to spacings exceeding 15 000 A can be clearly resolved. Work is about to start on the provision of a second superconducting wiggler with 6T field feeding five new experimental stations.

  3. Alternative Ultrafiltration Membrane Testing for the SRS Baseline Process

    SciTech Connect

    N. R. Mann; R. S. Herbst; T. G. Garn; M. R. Poirier; S. D. Fink

    2004-06-01

    The ability to more rapidly process high-level waste sludge and supernate, without sacrificing cost savings, continues to be a crucial challenge facing the Savannah River Site (SRS). There has, to date, not been any extensive investigation of alternative filter technologies for the SRS baseline process. To address this problem, a focused investigation into alternative, state-of-the art filtration technologies to facilitate the strontium and actinide removal process, which can be cost effectively implemented in existing facilities and current equipment designs, was completed. Filter technologies manufactured by Mott (0.1 µm and 0.5 µm) Graver (0.07 µm), Pall (0.1 µm and 0.8 µm) and GKN (0.1 µm) were evaluated. Membranes had a nominal inside diameter of 3/8 inches and an active membrane length of 2 feet. The investigation was performed in two phases. The first phase of testing evaluated the consistency or variability in flux through the different membranes using water and a standard 5.0 wt% strontium carbonate slurry. The second phase of testing evaluated the achievable permeate flux and clarity through the various membranes using the SRS average salt supernate simulant at solids loadings of 0.06, 0.29 and 4.5 wt%. Membrane variation data indicate that membranes having an asymmetric ceramic coating (Pall 0.1 µm and Graver 0.07 µm), typically displayed the lowest variability with water. Membranes without a ceramic asymmetric coating (Mott 0.5 µm and GKN 0.1 µm) displayed the highest variability. This is most likely associated with the experimental uncertainties in measuring large volumes of permeate in a short amount of time and to the impact of impurities in the water. In general, variability ranging from 4-56% was observed when using water for all membranes. In the case of variation testing using strontium carbonate, variability decreased to 3-12%. In addition, membrane structure or composition had little effect on the variability. Data obtained from SRS

  4. A lophotrochozoan-specific nuclear hormone receptor is required for reproductive system development in the planarian.

    PubMed

    Tharp, Marla E; Collins, James J; Newmark, Phillip A

    2014-12-01

    Germ cells of sexually reproducing organisms receive an array of cues from somatic tissues that instruct developmental processes. Although the nature of these signals differs amongst organisms, the importance of germline-soma interactions is a common theme. Recently, peptide hormones from the nervous system have been shown to regulate germ cell development in the planarian Schmidtea mediterranea; thus, we sought to investigate a second class of hormones with a conserved role in reproduction, the lipophilic hormones. In order to study these signals, we identified a set of putative lipophilic hormone receptors, known as nuclear hormone receptors, and analyzed their functions in reproductive development. We found one gene, nhr-1, belonging to a small class of functionally uncharacterized lophotrochozoan-specific receptors, to be essential for the development of differentiated germ cells. Upon nhr-1 knockdown, germ cells in the testes and ovaries fail to mature, and remain as undifferentiated germline stem cells. Further analysis revealed that nhr-1 mRNA is expressed in the accessory reproductive organs and is required for their development, suggesting that this transcription factor functions cell non-autonomously in regulating germ cell development. Our studies identify a role for nuclear hormone receptors in planarian reproductive maturation and reinforce the significance of germline-soma interactions in sexual reproduction across metazoans.

  5. Requirement for interleukin-1 to drive brain inflammation reveals tissue-specific mechanisms of innate immunity.

    PubMed

    Giles, James A; Greenhalgh, Andrew D; Davies, Claire L; Denes, Adam; Shaw, Tovah; Coutts, Graham; Rothwell, Nancy J; McColl, Barry W; Allan, Stuart M

    2015-02-01

    The immune system is implicated in a wide range of disorders affecting the brain and is, therefore, an attractive target for therapy. Interleukin-1 (IL-1) is a potent regulator of the innate immune system important for host defense but is also associated with injury and disease in the brain. Here, we show that IL-1 is a key mediator driving an innate immune response to inflammatory challenge in the mouse brain but is dispensable in extracerebral tissues including the lung and peritoneum. We also demonstrate that IL-1α is an important ligand contributing to the CNS dependence on IL-1 and that IL-1 derived from the CNS compartment (most likely microglia) is the major source driving this effect. These data reveal previously unknown tissue-specific requirements for IL-1 in driving innate immunity and suggest that IL-1-mediated inflammation in the brain could be selectively targeted without compromising systemic innate immune responses that are important for resistance to infection. This property could be exploited to mitigate injury- and disease-associated inflammation in the brain without increasing susceptibility to systemic infection, an important complication in several neurological disorders.

  6. Specific zinc-finger architecture required for HIV-1 nucleocapsid protein's nucleic acid chaperone function

    PubMed Central

    Williams, Mark C.; Gorelick, Robert J.; Musier-Forsyth, Karin

    2002-01-01

    The nucleocapsid protein (NC) of HIV type 1 (HIV-1) is a nucleic acid chaperone that facilitates the rearrangement of nucleic acid secondary structure during reverse transcription. HIV-1 NC contains two CCHC-type zinc binding domains. Here, we use optical tweezers to stretch single λ-DNA molecules through the helix-to-coil transition in the presence of wild-type and several mutant forms of HIV-1 NC with altered zinc-finger domains. Although all forms of NC lowered the cooperativity of the DNA helix–coil transition, subtle changes in the zinc-finger structures reduced NC's effect on the transition. The change in cooperativity of the DNA helix–coil transition correlates strongly with in vitro nucleic acid chaperone activity measurements and in vivo HIV-1 replication studies using the same NC mutants. Moreover, Moloney murine leukemia virus NC, which contains a single zinc finger, had little effect on transition cooperativity. These results suggest that a specific two-zinc-finger architecture is required to destabilize nucleic acids for optimal chaperone activity during reverse transcription in complex retroviruses such as HIV-1. PMID:12084921

  7. Stage-specific requirement for cyclin D1 in glial progenitor cells of the cerebral cortex.

    PubMed

    Nobs, Lionel; Baranek, Constanze; Nestel, Sigrun; Kulik, Akos; Kapfhammer, Josef; Nitsch, Cordula; Atanasoski, Suzana

    2014-05-01

    Despite the vast abundance of glial progenitor cells in the mouse brain parenchyma, little is known about the molecular mechanisms driving their proliferation in the adult. Here we unravel a critical role of the G1 cell cycle regulator cyclin D1 in controlling cell division of glial cells in the cortical grey matter. We detect cyclin D1 expression in Olig2-immunopositive (Olig2+) oligodendrocyte progenitor cells, as well as in Iba1+ microglia and S100β+ astrocytes in cortices of 3-month-old mice. Analysis of cyclin D1-deficient mice reveals a cell and stage-specific molecular control of cell cycle progression in the various glial lineages. While proliferation of fast dividing Olig2+ cells at early postnatal stages becomes gradually dependent on cyclin D1, this particular G1 regulator is strictly required for the slow divisions of Olig2+/NG2+ oligodendrocyte progenitors in the adult cerebral cortex. Further, we find that the population of mature oligodendrocytes is markedly reduced in the absence of cyclin D1, leading to a significant decrease in the number of myelinated axons in both the prefrontal cortex and the corpus callosum of 8-month-old mutant mice. In contrast, the pool of Iba1+ cells is diminished already at postnatal day 3 in the absence of cyclin D1, while the number of S100β+ astrocytes remains unchanged in the mutant.

  8. Label-free chemical imaging of live Euglena gracilis by high-speed SRS spectral microscopy (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Wakisaka, Yoshifumi; Suzuki, Yuta; Tokunaga, Kyoya; Hirose, Misa; Domon, Ryota; Akaho, Rina; Kuroshima, Mai; Tsumura, Norimichi; Shimobaba, Tomoyoshi; Iwata, Osamu; Suzuki, Kengo; Nakashima, Ayaka; Goda, Keisuke; Ozeki, Yasuyuki

    2016-03-01

    Microbes, especially microalgae, have recently been of great interest for developing novel biofuels, drugs, and biomaterials. Imaging-based screening of live cells can provide high selectivity and is attractive for efficient bio-production from microalgae. Although conventional cellular screening techniques use cell labeling, labeling of microbes is still under development and can interfere with their cellular functions. Furthermore, since live microbes move and change their shapes rapidly, a high-speed imaging technique is required to suppress motion artifacts. Stimulated Raman scattering (SRS) microscopy allows for label-free and high-speed spectral imaging, which helps us visualize chemical components inside biological cells and tissues. Here we demonstrate high-speed SRS imaging, with temporal resolution of 0.14 seconds, of intracellular distributions of lipid, polysaccharide, and chlorophyll concentrations in rapidly moving Euglena gracilis, a unicellular phytoflagellate. Furthermore, we show that our method allows us to analyze the amount of chemical components inside each living cell. Our results indicate that SRS imaging may be applied to label-free screening of living microbes based on chemical information.

  9. Long-term outcome of stereotactic radiosurgery (Srs) in patients with acoustic neuromas

    SciTech Connect

    Combs, Stephanie E. . E-mail: Stephanie.Combs@med.uni-heidelberg.de; Thilmann, Christoph; Debus, Juergen; Schulz-Ertner, Daniela

    2006-04-01

    Purpose: To evaluate the effectiveness and long-term outcome of stereotactic radiosurgery (SRS) for acoustic neuromas (AN). Patients and Methods: Between 1990 and 2001, we treated 26 patients with 27 AN with SRS. Two patients suffered from neurofibromatosis type 2. Before SRS, a subtotal or total resection had been performed in 3 and in 5 patients, respectively. For SRS, a median single dose of 13 Gy/80% isodose was applied. Results: The overall actuarial 5-year and 10-year tumor control probability in all patients was 91%. Two patients developed tumor progression after SRS at 36 and 48 months. Nineteen patients (73%) were at risk of treatment-related facial nerve toxicity; of these, 1 patient developed a complete facial nerve palsy after SRS (5%). A total of 93% of the lesions treated were at risk of radiation-induced trigeminal neuralgia. Two patients (8%) developed mild dysesthesia of the trigeminal nerve after SRS. The hearing preservation rate in patients with useful hearing before SRS was 55% at 9 years. Conclusion:: Stereotactic radiosurgery results in good local control rates of AN and the risk of cranial nerve toxicities is acceptable. As toxicity is lower with fractionated stereotactic radiotherapy, SRS should be reserved for smaller lesions.

  10. 49 CFR 179.221 - Individual specification requirements applicable to tank car tanks consisting of an inner...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Individual specification requirements applicable to tank car tanks consisting of an inner container supported within an outer shell. 179.221 Section... within an outer shell....

  11. 10 CFR 110.32 - Information required in an application for a specific license/NRC Form 7.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 2 2011-01-01 2011-01-01 false Information required in an application for a specific license/NRC Form 7. 110.32 Section 110.32 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) EXPORT AND IMPORT OF NUCLEAR EQUIPMENT AND MATERIAL Licenses § 110.32 Information required in an application for a specific license/NRC Form 7. (a) Name...

  12. rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco.

    PubMed

    Cui, X Y; Chen, Z Y; Wu, L; Liu, X Q; Dong, Y Y; Wang, F W; Li, H Y

    2015-07-03

    The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13- and 7.40-fold higher in leaves than in stems and roots, respectively. Moreover, GUS activity was stimulated by light. In conclusion, spatial and light regulation of the soybean rbcS promoter was observed in N. tabacum, thus illustrating a leaf-specific and light-induced promoter.

  13. rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco.

    PubMed

    Cui, X Y; Chen, Z Y; Wu, L; Liu, X Q; Dong, Y Y; Wang, F W; Li, H Y

    2015-01-01

    The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13- and 7.40-fold higher in leaves than in stems and roots, respectively. Moreover, GUS activity was stimulated by light. In conclusion, spatial and light regulation of the soybean rbcS promoter was observed in N. tabacum, thus illustrating a leaf-specific and light-induced promoter. PMID:26214418

  14. Cytoplasmic nanojunctions between lysosomes and sarcoplasmic reticulum are required for specific calcium signaling

    PubMed Central

    Fameli, Nicola; Ogunbayo, Oluseye A.

    2014-01-01

    Herein we demonstrate how nanojunctions between lysosomes and sarcoplasmic reticulum (L-SR junctions) serve to couple lysosomal activation to regenerative, ryanodine receptor-mediated cellular Ca 2+ waves. In pulmonary artery smooth muscle cells (PASMCs) it has been proposed that nicotinic acid adenine dinucleotide phosphate (NAADP) triggers increases in cytoplasmic Ca 2+ via L-SR junctions, in a manner that requires initial Ca 2+ release from lysosomes and subsequent Ca 2+-induced Ca 2+ release (CICR) via ryanodine receptor (RyR) subtype 3 on the SR membrane proximal to lysosomes. L-SR junction membrane separation has been estimated to be < 400 nm and thus beyond the resolution of light microscopy, which has restricted detailed investigations of the junctional coupling process. The present study utilizes standard and tomographic transmission electron microscopy to provide a thorough ultrastructural characterization of the L-SR junctions in PASMCs. We show that L-SR nanojunctions are prominent features within these cells and estimate that the junctional membrane separation and extension are about 15 nm and 300 nm, respectively. Furthermore, we develop a quantitative model of the L-SR junction using these measurements, prior kinetic and specific Ca 2+ signal information as input data. Simulations of NAADP-dependent junctional Ca 2+ transients demonstrate that the magnitude of these signals can breach the threshold for CICR via RyR3. By correlation analysis of live cell Ca 2+ signals and simulated Ca 2+ transients within L-SR junctions, we estimate that “trigger zones” comprising 60–100 junctions are required to confer a signal of similar magnitude. This is compatible with the 110 lysosomes/cell estimated from our ultrastructural observations. Most importantly, our model shows that increasing the L-SR junctional width above 50 nm lowers the magnitude of junctional [Ca 2+] such that there is a failure to breach the threshold for CICR via RyR3. L-SR junctions are

  15. 49 CFR 179.201 - Individual specification requirements applicable to non-pressure tank car tanks.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... to non-pressure tank car tanks. 179.201 Section 179.201 Transportation Other Regulations Relating to... MATERIALS REGULATIONS SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes... car tanks....

  16. 49 CFR 178.345 - General design and construction requirements applicable to Specification DOT 406 (§ 178.346), DOT...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... applicable to Specification DOT 406 (§ 178.346), DOT 407 (§ 178.347), and DOT 412 (§ 178.348) cargo tank....345 General design and construction requirements applicable to Specification DOT 406 (§ 178.346), DOT 407 (§ 178.347), and DOT 412 (§ 178.348) cargo tank motor vehicles....

  17. Permissivity of the biphenyl-specific aerobic bacterial metabolic pathway towards analogues with various steric requirements.

    PubMed

    Overwin, Heike; Standfuß-Gabisch, Christine; González, Myriam; Méndez, Valentina; Seeger, Michael; Reichelt, Joachim; Wray, Victor; Hofer, Bernd

    2015-09-01

    It has repeatedly been shown that aryl-hydroxylating dioxygenases do not possess a very high substrate specificity. To gain more insight into this phenomenon, we examined two powerful biphenyl dioxygenases, the well-known wild-type enzyme from Burkholderia xenovorans LB400 (BphA-LB400) and a hybrid enzyme, based on a dioxygenase from Pseudomonas sp. B4-Magdeburg (BphA-B4h), for their abilities to dioxygenate a selection of eight biphenyl analogues in which the second aromatic ring was replaced by aliphatic as well as aliphatic/aromatic moieties, reflecting a variety of steric requirements. Interestingly, both enzymes were able to catalyse transformation of almost all of these compounds. While the products formed were identical, major differences were observed in transformation rates. In most cases, BphA-B4h proved to be a significantly more powerful catalyst than BphA-LB400. NMR characterization of the reaction products showed that the metabolite obtained from biphenylene underwent angular dioxygenation, whereas all other compounds were subject to lateral dioxygenation at ortho and meta carbons. Subsequent growth studies revealed that both dioxygenase source strains were able to utilize several of the biphenyl analogues as sole sources of carbon and energy. Therefore, prototype BphBCD enzymes of the biphenyl degradative pathway were examined for their ability to further catabolize the lateral dioxygenation products. All of the ortho- and meta-hydroxylated compounds were converted to acids, showing that this pathway is quite permissive, enabling catalysis of the turnover of a fairly wide variety of metabolites. PMID:26297047

  18. Permissivity of the biphenyl-specific aerobic bacterial metabolic pathway towards analogues with various steric requirements.

    PubMed

    Overwin, Heike; Standfuß-Gabisch, Christine; González, Myriam; Méndez, Valentina; Seeger, Michael; Reichelt, Joachim; Wray, Victor; Hofer, Bernd

    2015-09-01

    It has repeatedly been shown that aryl-hydroxylating dioxygenases do not possess a very high substrate specificity. To gain more insight into this phenomenon, we examined two powerful biphenyl dioxygenases, the well-known wild-type enzyme from Burkholderia xenovorans LB400 (BphA-LB400) and a hybrid enzyme, based on a dioxygenase from Pseudomonas sp. B4-Magdeburg (BphA-B4h), for their abilities to dioxygenate a selection of eight biphenyl analogues in which the second aromatic ring was replaced by aliphatic as well as aliphatic/aromatic moieties, reflecting a variety of steric requirements. Interestingly, both enzymes were able to catalyse transformation of almost all of these compounds. While the products formed were identical, major differences were observed in transformation rates. In most cases, BphA-B4h proved to be a significantly more powerful catalyst than BphA-LB400. NMR characterization of the reaction products showed that the metabolite obtained from biphenylene underwent angular dioxygenation, whereas all other compounds were subject to lateral dioxygenation at ortho and meta carbons. Subsequent growth studies revealed that both dioxygenase source strains were able to utilize several of the biphenyl analogues as sole sources of carbon and energy. Therefore, prototype BphBCD enzymes of the biphenyl degradative pathway were examined for their ability to further catabolize the lateral dioxygenation products. All of the ortho- and meta-hydroxylated compounds were converted to acids, showing that this pathway is quite permissive, enabling catalysis of the turnover of a fairly wide variety of metabolites.

  19. TESTING OF ENHANCED CHEMICAL CLEANING OF SRS ACTUAL WASTE TANK 5F AND TANK 12H SLUDGES

    SciTech Connect

    Martino, C.; King, W.

    2011-08-22

    Forty three of the High Level Waste (HLW) tanks at the Savannah River Site (SRS) have internal structures that hinder removal of the last approximately five thousand gallons of waste sludge solely by mechanical means. Chemical cleaning can be utilized to dissolve the sludge heel with oxalic acid (OA) and pump the material to a separate waste tank in preparation for final disposition. This dissolved sludge material is pH adjusted downstream of the dissolution process, precipitating the sludge components along with sodium oxalate solids. The large quantities of sodium oxalate and other metal oxalates formed impact downstream processes by requiring additional washing during sludge batch preparation and increase the amount of material that must be processed in the tank farm evaporator systems and the Saltstone Processing Facility. Enhanced Chemical Cleaning (ECC) was identified as a potential method for greatly reducing the impact of oxalate additions to the SRS Tank Farms without adding additional components to the waste that would extend processing or increase waste form volumes. In support of Savannah River Site (SRS) tank closure efforts, the Savannah River National Laboratory (SRNL) conducted Real Waste Testing (RWT) to evaluate an alternative to the baseline 8 wt. % OA chemical cleaning technology for tank sludge heel removal. The baseline OA technology results in the addition of significant volumes of oxalate salts to the SRS tank farm and there is insufficient space to accommodate the neutralized streams resulting from the treatment of the multiple remaining waste tanks requiring closure. ECC is a promising alternative to bulk OA cleaning, which utilizes a more dilute OA (nominally 2 wt. % at a pH of around 2) and an oxalate destruction technology. The technology is being adapted by AREVA from their decontamination technology for Nuclear Power Plant secondary side scale removal. This report contains results from the SRNL small scale testing of the ECC process

  20. 40 CFR 152.95 - Citation of all studies in the Agency's files pertinent to a specific data requirement.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Citation of all studies in the Agency's files pertinent to a specific data requirement. 152.95 Section 152.95 Protection of Environment... PROCEDURES Satisfaction of Data Requirements and Protection of Data Submitters' Rights § 152.95 Citation...

  1. Thirty-year solid waste generation forecast for facilities at SRS

    SciTech Connect

    Not Available

    1994-07-01

    The information supplied by this 30-year solid waste forecast has been compiled as a source document to the Waste Management Environmental Impact Statement (WMEIS). The WMEIS will help to select a sitewide strategic approach to managing present and future Savannah River Site (SRS) waste generated from ongoing operations, environmental restoration (ER) activities, transition from nuclear production to other missions, and decontamination and decommissioning (D&D) programs. The EIS will support project-level decisions on the operation of specific treatment, storage, and disposal facilities within the near term (10 years or less). In addition, the EIS will provide a baseline for analysis of future waste management activities and a basis for the evaluation of the specific waste management alternatives. This 30-year solid waste forecast will be used as the initial basis for the EIS decision-making process. The Site generates and manages many types and categories of waste. With a few exceptions, waste types are divided into two broad groups-high-level waste and solid waste. High-level waste consists primarily of liquid radioactive waste, which is addressed in a separate forecast and is not discussed further in this document. The waste types discussed in this solid waste forecast are sanitary waste, hazardous waste, low-level mixed waste, low-level radioactive waste, and transuranic waste. As activities at SRS change from primarily production to primarily decontamination and decommissioning and environmental restoration, the volume of each waste s being managed will change significantly. This report acknowledges the changes in Site Missions when developing the 30-year solid waste forecast.

  2. Requirements Analysis Study for Master Pump Shutdown System Project Development Specification [SEC 1 and 2

    SciTech Connect

    BEVINS, R.R.

    2000-03-24

    This document has been updated during the definitive design portion of the first phase of the W-314 Project to capture additional software requirements and is planned to be updated during the second phase of the W-314 Project to cover the second phase of the Project's scope. The objective is to provide requirement traceability by recording the analysis/basis for the functional descriptions of the master pump shutdown system. This document identifies the sources of the requirements and/or how these were derived. Each requirement is validated either by quoting the source or an analysis process involving the required functionality, performance characteristics, operations input or engineering judgment.

  3. Clemson final report: High temperature formulations for SRS soils

    SciTech Connect

    Schumacher, R.F.

    1997-03-11

    This study was undertaken to demonstrate the application of a DC arc melter to in-situ vitrification of SRS soils. The melter that was available at the DOE/Industrial Vitrification Laboratory at Clemson University was equipped with opposing solid electrodes. To simulate field conditions, two hollow electrode configurations were evaluated which allowed fluxes to be injected into the melter while the soils were being vitrified. the first 4 runs utilized pre-blended flux (two runs) and attempted flux injection (two runs). These runs were terminated prematurely due to offgas sampling problems and melt freezing. The remaining four runs utilized a different electrode geometry, and the runs were not interrupted to change out the offgas sampling apparatus. These runs were conducted successfully.

  4. APPLICATION OF NONSPHERICAL FISSILE CONFIGURATION IN WASTE CONTAINERS AT SRS

    SciTech Connect

    Eghbali, D; Michelle Abney, M

    2007-01-03

    Transuranic (TRU) solid waste that has been generated as a result of the production of nuclear material for the United States defense program at the Savannah River Site (SRS) has been stored in more than 30,000 55-gallon drums and carbon steel boxes since 1953. Nearly two thirds of those containers have been processed and shipped to the Waste Isolation Pilot Plant. Among the containers assayed so far, the results indicate several drums with fissile inventories significantly higher (600-1000 fissile grams equivalent (FGE) {sup 239}Pu) than their original assigned values. While part of this discrepancy can be attributed to the past limited assay capabilities, human errors are believed to be the primary contributor. This paper summarizes the application of nonspherical fissile material configuration in waste containers, resulting in less restrictive mass and spacing limits, increased storage capacity, and several administrative controls for handling and storage of waste containers being modified without compromising safety.

  5. Experimental Determination and Thermodynamic Modeling of Electrical Conductivity of SRS Waste Tank Supernate

    SciTech Connect

    Pike, J.; Reboul, S.

    2015-06-01

    SRS High Level Waste Tank Farm personnel rely on conductivity probes for detection of incipient overflow conditions in waste tanks. Minimal information is available concerning the sensitivity that must be achieved such that that liquid detection is assured. Overly sensitive electronics results in numerous nuisance alarms for these safety-related instruments. In order to determine the minimum sensitivity required of the probe, Tank Farm Engineering personnel need adequate conductivity data to improve the existing designs. Little or no measurements of liquid waste conductivity exist; however, the liquid phase of the waste consists of inorganic electrolytes for which the conductivity may be calculated. Savannah River Remediation (SRR) Tank Farm Facility Engineering requested SRNL to determine the conductivity of the supernate resident in SRS waste Tank 40 experimentally as well as computationally. In addition, SRNL was requested to develop a correlation, if possible, that would be generally applicable to liquid waste resident in SRS waste tanks. A waste sample from Tank 40 was analyzed for composition and electrical conductivity as shown in Table 4-6, Table 4-7, and Table 4-9. The conductivity for undiluted Tank 40 sample was 0.087 S/cm. The accuracy of OLI Analyzer™ was determined using available literature data. Overall, 95% of computed estimates of electrical conductivity are within ±15% of literature values for component concentrations from 0 to 15 M and temperatures from 0 to 125 °C. Though the computational results are generally in good agreement with the measured data, a small portion of literature data deviates as much as ±76%. A simplified model was created that can be used readily to estimate electrical conductivity of waste solution in computer spreadsheets. The variability of this simplified approach deviates up to 140% from measured values. Generally, this model can be applied to estimate the conductivity within a factor of two. The comparison of the

  6. Colloid and ionic tracer migration within SRS sediments: Final summary

    SciTech Connect

    Strom, R.N.; Seaman, J.C.; Bertsch, P.M.; Miller, W.P.

    1996-04-09

    The generation of a stable colloidal suspension in geologic materials has a number of environmental implications. Mobile colloids may act as vectors for the transport of adsorbed contaminants through soils and within aquifers and can cause serious problems related to well monitoring and formation permeability in an injections well system. Colloid-facilitated transport has been implicated in the migration of contaminants from seepage basins on the Department of Energy`s Savannah River Site (SRS) at a rate greater than was predicted in two- phase transport models. From 1955 to 1988, seepage basins overlying the water-table aquifer received acidic wastes containing high levels of Na+ and nitric acid, as well as trace radionuclides and metals from the nuclear materials processing facilities. Numerical simulations predicted that metal contaminants would not reach the water table, but measurable quantities of these contaminants have been detected in monitoring wells down gradient from the basins. Lack of agreement between predicted and observed contaminant migration in this and other studies has been attributed to both local non equilibrium situation, preferential flow paths within the geologic material, and to transport of the contaminant in association with a mobile solid phase, i.e. dispersed colloids. Additionally, the association of contaminants with a mobile colloidal phase has important ramifications for groundwater sampling on SRS intended to evaluate the potential environmental hazards of a given contaminant. As part of the F- and H-Area reclamation project, the Department of Energy has proposed the capture and treatment of the contaminant plume followed by reinjection of the treated water into the water table and upper confined aquifers. (Abstract Truncated)

  7. Requirements Analysis Study for Master Pump Shutdown System Project Development Specification [SEC 1 and 2

    SciTech Connect

    BEVINS, R.R.

    2000-09-20

    This study is a requirements document that presents analysis for the functional description for the master pump shutdown system. This document identifies the sources of the requirements and/or how these were derived. Each requirement is validated either by quoting the source or an analysis process involving the required functionality, performance characteristics, operations input or engineering judgment. The requirements in this study apply to the first phase of the W314 Project. This document has been updated during the definitive design portion of the first phase of the W314 Project to capture additional software requirements and is planned to be updated during the second phase of the W314 Project to cover the second phase of the project's scope.

  8. Some Specific CASL Requirements for Advanced Multiphase Flow Simulation of Light Water Reactors

    SciTech Connect

    R. A. Berry

    2010-11-01

    Because of the diversity of physical phenomena occuring in boiling, flashing, and bubble collapse, and of the length and time scales of LWR systems, it is imperative that the models have the following features: • Both vapor and liquid phases (and noncondensible phases, if present) must be treated as compressible. • Models must be mathematically and numerically well-posed. • The models methodology must be multi-scale. A fundamental derivation of the multiphase governing equation system, that should be used as a basis for advanced multiphase modeling in LWR coolant systems, is given in the Appendix using the ensemble averaging method. The remainder of this work focuses specifically on the compressible, well-posed, and multi-scale requirements of advanced simulation methods for these LWR coolant systems, because without these are the most fundamental aspects, without which widespread advancement cannot be claimed. Because of the expense of developing multiple special-purpose codes and the inherent inability to couple information from the multiple, separate length- and time-scales, efforts within CASL should be focused toward development of a multi-scale approaches to solve those multiphase flow problems relevant to LWR design and safety analysis. Efforts should be aimed at developing well-designed unified physical/mathematical and high-resolution numerical models for compressible, all-speed multiphase flows spanning: (1) Well-posed general mixture level (true multiphase) models for fast transient situations and safety analysis, (2) DNS (Direct Numerical Simulation)-like models to resolve interface level phenmena like flashing and boiling flows, and critical heat flux determination (necessarily including conjugate heat transfer), and (3) Multi-scale methods to resolve both (1) and (2) automatically, depending upon specified mesh resolution, and to couple different flow models (single-phase, multiphase with several velocities and pressures, multiphase with single

  9. Justification for Continued Operation of the SRS Saltstone Facility (Z-Area)

    SciTech Connect

    Wagner, W.A.

    1999-01-20

    Saltstone Production and Disposal Facilities (Z-Area) are a part of the Defense Waste Processing Facilities (DWPF). Z-Area facilities are just one segment of an integrated waste management and disposal system located at the Savannah River Site (SRS). The bases for the Justification of Continuing Operations (JCO) of the Saltstone Production and Disposal Facilities (Z-Area) at SRS are provided.

  10. 49 CFR 179.101 - Individual specification requirements applicable to pressure tank car tanks.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... to pressure tank car tanks. 179.101 Section 179.101 Transportation Other Regulations Relating to... MATERIALS REGULATIONS SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT... tank car tanks. Editorial Note: At 66 FR 45186, Aug. 28, 2001, an amendment published amending a...

  11. 49 CFR 173.472 - Requirements for exporting DOT Specification Type B and fissile packages.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of the quality assurance program required by 10 CFR part 71, subpart H, or 49 CFR 173.474 and 173.475... the package identification marking indicated in the U.S. Competent Authority Certificate. (e) Before... must be in triplicate and must include a description of the quality assurance program required by...

  12. 24 CFR 200.937 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... requirements under HUD building product standards and certification program for plastic bathtub units, plastic shower receptors and stalls, plastic lavatories, plastic water closet bowls and tanks. 200.937 Section... procedural requirements under HUD building product standards and certification program for plastic...

  13. 24 CFR 200.937 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... requirements under HUD building product standards and certification program for plastic bathtub units, plastic shower receptors and stalls, plastic lavatories, plastic water closet bowls and tanks. 200.937 Section... procedural requirements under HUD building product standards and certification program for plastic...

  14. 24 CFR 200.937 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... requirements under HUD building product standards and certification program for plastic bathtub units, plastic shower receptors and stalls, plastic lavatories, plastic water closet bowls and tanks. 200.937 Section... procedural requirements under HUD building product standards and certification program for plastic...

  15. 24 CFR 200.937 - Supplementary specific procedural requirements under HUD building product standards and...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... requirements under HUD building product standards and certification program for plastic bathtub units, plastic shower receptors and stalls, plastic lavatories, plastic water closet bowls and tanks. 200.937 Section... procedural requirements under HUD building product standards and certification program for plastic...

  16. 40 CFR 63.1103 - Source category-specific applicability, definitions, and requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... occur prior to the polymer reactor. (3) Requirements. Table 1 of this section specifies the acetal... point by meeting the applicable requirements specified in table 1 of this section. Table 1 to § 63.1103... Enclosure” in 40 CFR 52.741, appendix B. (iii) The enclosure may have permanent or temporary openings...

  17. 40 CFR 270.23 - Specific part B information requirements for miscellaneous units.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., and closed to comply with the requirements of §§ 264.601 and 264.602; and (3) For disposal units, a... dispose of hazardous waste in miscellaneous units must provide the following additional information: (a) A... requirements for miscellaneous units. 270.23 Section 270.23 Protection of Environment ENVIRONMENTAL...

  18. 40 CFR 62.14620 - What site-specific documentation is required?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... POLLUTANTS Federal Plan Requirements for Commercial and Industrial Solid Waste Incineration Units That...) Procedures for receiving, handling, and charging waste. (3) Incinerator startup, shutdown, and malfunction... limits. (7) Reporting and recordkeeping procedures. (8) The waste management plan required under §§...

  19. 40 CFR 62.14620 - What site-specific documentation is required?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... POLLUTANTS Federal Plan Requirements for Commercial and Industrial Solid Waste Incineration Units That...) Procedures for receiving, handling, and charging waste. (3) Incinerator startup, shutdown, and malfunction... limits. (7) Reporting and recordkeeping procedures. (8) The waste management plan required under §§...

  20. 49 CFR 171.23 - Requirements for specific materials and packagings transported under the ICAO Technical...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., may be filled with a gas in the United States and offered for transportation and transported for... Administrator; (ii) In addition to other requirements of this subchapter, the maximum filling density, service pressure, and pressure relief drive for each cylinder conform to the requirements of this part for the...

  1. 10 CFR 72.236 - Specific requirements for spent fuel storage cask approval and fabrication.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR... spent fuel (i.e., intact assembly or consolidated fuel rods), the inerting atmosphere requirements. (b... removal of the stored spent fuel from a reactor site, transportation, and ultimate disposition by...

  2. Rbfox proteins regulate tissue-specific alternative splicing of Mef2D required for muscle differentiation.

    PubMed

    Runfola, Valeria; Sebastian, Soji; Dilworth, F Jeffrey; Gabellini, Davide

    2015-02-15

    Among the Mef2 family of transcription factors, Mef2D is unique in that it undergoes tissue-specific splicing to generate an isoform that is essential for muscle differentiation. However, the mechanisms mediating this muscle-specific processing of Mef2D remain unknown. Using bioinformatics, we identified Rbfox proteins as putative modulators of Mef2D muscle-specific splicing. Accordingly, we found direct and specific Rbfox1 and Rbfox2 binding to Mef2D pre-mRNA in vivo. Gain- and loss-of-function experiments demonstrated that Rbfox1 and Rbfox2 cooperate in promoting Mef2D splicing and subsequent myogenesis. Thus, our findings reveal a new role for Rbfox proteins in regulating myogenesis through activation of essential muscle-specific splicing events.

  3. Evaluation of specific requirements for unfiltered surface-water supplies. Draft report

    SciTech Connect

    Not Available

    1988-09-13

    The Amendments to the Safe Drinking Water Act passed in June 1986 (PL 99-339) require that the United States Environmental Protection Agency (USEPA) promulgate a National Primary Drinking Water Regulation (NPDWR) by December 1987, specifying criteria under which filtration (including pretreatment, coagulation and sedimentation as appropriate) would be required as a treatment technique for public-water systems supplied by surface-water sources. The proposed Surface Water Treatment Rule (SWTR), developed to comply with this requirement, includes conditions for which filtration would be required as a treatment technique for systems utilizing a surface water source, as well as conditions for which disinfection alone would be the only required treatment. The purpose of the report is to evaluate the criteria presented in the proposed SWTR (52 Federal Register 42 178, November 3, 1987) and the draft Guidance Manual (October 8, 1987) which pertain to avoiding filtration.

  4. Upgrade of the TOTEM DAQ using the Scalable Readout System (SRS)

    NASA Astrophysics Data System (ADS)

    Quinto, M.; Cafagna, F.; Fiergolski, A.; Radicioni, E.

    2013-11-01

    The main goals of the TOTEM Experiment at the LHC are the measurements of the elastic and total p-p cross sections and the studies of the diffractive dissociation processes. At LHC, collisions are produced at a rate of 40 MHz, imposing strong requirements for the Data Acquisition Systems (DAQ) in terms of trigger rate and data throughput. The TOTEM DAQ adopts a modular approach that, in standalone mode, is based on VME bus system. The VME based Front End Driver (FED) modules, host mezzanines that receive data through optical fibres directly from the detectors. After data checks and formatting are applied in the mezzanine, data is retransmitted to the VME interface and to another mezzanine card plugged in the FED module. The VME bus maximum bandwidth limits the maximum first level trigger (L1A) to 1 kHz rate. In order to get rid of the VME bottleneck and improve scalability and the overall capabilities of the DAQ, a new system was designed and constructed based on the Scalable Readout System (SRS), developed in the framework of the RD51 Collaboration. The project aims to increase the efficiency of the actual readout system providing higher bandwidth, and increasing data filtering, implementing a second-level trigger event selection based on hardware pattern recognition algorithms. This goal is to be achieved preserving the maximum back compatibility with the LHC Timing, Trigger and Control (TTC) system as well as with the CMS DAQ. The obtained results and the perspectives of the project are reported. In particular, we describe the system architecture and the new Opto-FEC adapter card developed to connect the SRS with the FED mezzanine modules. A first test bench was built and validated during the last TOTEM data taking period (February 2013). Readout of a set of 3 TOTEM Roman Pot silicon detectors was carried out to verify performance in the real LHC environment. In addition, the test allowed a check of data consistency and quality.

  5. ACTUAL-WASTE TESTING OF ULTRAVIOLET LIGHT TO AUGMENT THE ENHANCED CHEMICAL CLEANING OF SRS SLUDGE

    SciTech Connect

    Martino, C.; King, W.; Ketusky, E.

    2012-07-10

    In support of Savannah River Site (SRS) tank closure efforts, the Savannah River National Laboratory (SRNL) conducted Real Waste Testing (RWT) to evaluate Enhanced Chemical Cleaning (ECC), an alternative to the baseline 8 wt% oxalic acid (OA) chemical cleaning technology for tank sludge heel removal. ECC utilizes a more dilute OA solution (2 wt%) and an oxalate destruction technology using ozonolysis with or without the application of ultraviolet (UV) light. SRNL conducted tests of the ECC process using actual SRS waste material from Tanks 5F and 12H. The previous phase of testing involved testing of all phases of the ECC process (sludge dissolution, OA decomposition, product evaporation, and deposition tank storage) but did not involve the use of UV light in OA decomposition. The new phase of testing documented in this report focused on the use of UV light to assist OA decomposition, but involved only the OA decomposition and deposition tank portions of the process. Compared with the previous testing at analogous conditions without UV light, OA decomposition with the use of UV light generally reduced time required to reach the target of <100 mg/L oxalate. This effect was the most pronounced during the initial part of the decomposition batches, when pH was <4. For the later stages of each OA decomposition batch, the increase in OA decomposition rate with use of the UV light appeared to be minimal. Testing of the deposition tank storage of the ECC product resulted in analogous soluble concentrations regardless of the use or non-use of UV light in the ECC reactor.

  6. 40 CFR 270.17 - Specific part B information requirements for surface impoundments.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... overtopping; and (7) Structural integrity of dikes; (c) A description of how each surface impoundment... certification by a qualified engineer which attests to the structural integrity of each dike, as required...

  7. 10 CFR 73.23 - Protection of Safeguards Information-Modified Handling: Specific requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... CFR 73.59 are exempt from the criminal history records check and background check requirements in... access Safeguards Information by gaining access to retained memory or network connectivity). (f)...

  8. Experimental development based on mapping rule between requirements analysis model and web framework specific design model.

    PubMed

    Okuda, Hirotaka; Ogata, Shinpei; Matsuura, Saeko

    2013-12-01

    Model Driven Development is a promising approach to develop high quality software systems. We have proposed a method of model-driven requirements analysis using Unified Modeling Language (UML). The main feature of our method is to automatically generate a Web user interface prototype from UML requirements analysis model so that we can confirm validity of input/output data for each page and page transition on the system by directly operating the prototype. We proposes a mapping rule in which design information independent of each web application framework implementation is defined based on the requirements analysis model, so as to improve the traceability to the final product from the valid requirements analysis model. This paper discusses the result of applying our method to the development of a Group Work Support System that is currently running in our department.

  9. Waste Receiving and Processing Facility Module 1 Data Management System software requirements specification

    SciTech Connect

    Rosnick, C.K.

    1996-04-19

    This document provides the software requirements for Waste Receiving and Processing (WRAP) Module 1 Data Management System (DMS). The DMS is one of the plant computer systems for the new WRAP 1 facility (Project W-0126). The DMS will collect, store and report data required to certify the low level waste (LLW) and transuranic (TRU) waste items processed at WRAP 1 as acceptable for shipment, storage, or disposal.

  10. Waste Receiving and Processing Facility Module 1 Data Management System Software Requirements Specification

    SciTech Connect

    Brann, E.C. II

    1994-09-09

    This document provides the software requirements for Waste Receiving and Processing (WRAP) Module 1 Data Management System (DMS). The DMS is one of the plant computer systems for the new WRAP 1 facility (Project W-026). The DMS will collect, store and report data required to certify the low level waste (LLW) and transuranic (TRU) waste items processed at WRAP 1 as acceptable for shipment, storage, or disposal.

  11. Reduced cerebral infection of Neospora caninum in BALB/c mice vaccinated with recombinant Brucella abortus RB51 strains expressing N. caninum SRS2 and GRA7 proteins.

    PubMed

    Vemulapalli, Ramesh; Sanakkayala, Neelima; Gulani, Jatinder; Schurig, Gerhardt G; Boyle, Stephen M; Lindsay, David S; Sriranganathan, Nammalwar

    2007-09-30

    Neospora caninum, an obligate intracellular protozoan parasite, is the causative agent of bovine neosporosis, an important disease affecting the reproductive performance of cattle worldwide. Currently there is no effective vaccine available to prevent N. caninum infection in cattle. In this study, we examined the feasibility of developing a live, recombinant N. caninum vaccine using Brucella abortus vaccine strain RB51 as the expression and delivery vector. We generated two recombinant RB51 strains each expressing SRS2 (RB51/SRS2) or GRA7 (RB51/GRA7) antigens of N. caninum. BALB/c mice immunized by single intraperitoneal inoculation of the recombinant RB51 strains developed IgG antibodies specific to the respective N. caninum antigen. In vitro stimulation of splenocytes from the vaccinated mice with specific antigen resulted in the production of interferon-gamma, but not IL-5 or IL-10, suggesting the development of a Th1 type immune response. Upon challenge with N. caninum tachyzoites, mice vaccinated with strain RB51/SRS2, but not RB51/GRA7, showed significant resistance to cerebral infection when compared to the RB51 vaccinated mice, as determined by the tissue parasite load using a real-time quantitative TaqMan assay. Interestingly, mice vaccinated with either strain RB51 or RB51/GRA7 also contained significantly lower parasite burden in their brains compared to those inoculated with saline. Mice vaccinated with strain RB51/SRS2 or RB51/GRA7 were protected to the same extent as the strain RB51 vaccinated mice against challenge with B. abortus virulent strain 2308. These results suggest that a recombinant RB51 strain expressing an appropriate protective antigen(s), such as SRS2 of N. caninum, can confer protection against both neosporosis and brucellosis.

  12. Tethys: The Marine and Hydrokinetic Technology Environmental Impacts Knowledge Management System -- Requirements Specification -- Version 1.0

    SciTech Connect

    Butner, R. Scott; Snowden-Swan, Lesley J.; Ellis, Peter C.

    2010-11-09

    The marine and hydrokinetic (MHK) environmental impacts knowledge management system (KMS), dubbed Tethys after the mythical Greek goddess of the seas, is being developed for the U.S. Department of Energy (DOE) Office of Energy Efficiency and Renewable Energy Wind and Hydropower Technologies Program (WHTP) by Pacific Northwest National Laboratory (PNNL). This requirements specification establishes the essential capabilities required of Tethys and clarifies for WHTP and the Tethys development team the results that must be achieved by the system.

  13. The cytoskeletal regulator Genghis khan is required for columnar target specificity in the Drosophila visual system

    PubMed Central

    Gontang, Allison C.; Hwa, Jennifer J.; Mast, Joshua D.; Schwabe, Tina; Clandinin, Thomas R.

    2011-01-01

    A defining characteristic of neuronal cell type is the growth of axons and dendrites into specific layers and columns of the brain. Although differences in cell surface receptors and adhesion molecules are known to cause differences in synaptic specificity, differences in downstream signaling mechanisms that determine cell type-appropriate targeting patterns are unknown. Using a forward genetic screen in Drosophila, we identify the GTPase effector Genghis khan (Gek) as playing a crucial role in the ability of a subset of photoreceptor (R cell) axons to innervate appropriate target columns. In particular, single-cell mosaic analyses demonstrate that R cell growth cones lacking Gek function grow to the appropriate ganglion, but frequently fail to innervate the correct target column. Further studies reveal that R cell axons lacking the activity of the small GTPase Cdc42 display similar defects, providing evidence that these proteins regulate a common set of processes. Gek is expressed in all R cells, and a detailed structure-function analysis reveals a set of regulatory domains with activities that restrict Gek function to the growth cone. Although Gek does not normally regulate layer-specific targeting, ectopic expression of Gek is sufficient to alter the targeting choices made by another R cell type, the targeting of which is normally Gek independent. Thus, specific regulation of cytoskeletal responses to targeting cues is necessary for cell type-appropriate synaptic specificity. PMID:22007130

  14. The cytoskeletal regulator Genghis khan is required for columnar target specificity in the Drosophila visual system.

    PubMed

    Gontang, Allison C; Hwa, Jennifer J; Mast, Joshua D; Schwabe, Tina; Clandinin, Thomas R

    2011-11-01

    A defining characteristic of neuronal cell type is the growth of axons and dendrites into specific layers and columns of the brain. Although differences in cell surface receptors and adhesion molecules are known to cause differences in synaptic specificity, differences in downstream signaling mechanisms that determine cell type-appropriate targeting patterns are unknown. Using a forward genetic screen in Drosophila, we identify the GTPase effector Genghis khan (Gek) as playing a crucial role in the ability of a subset of photoreceptor (R cell) axons to innervate appropriate target columns. In particular, single-cell mosaic analyses demonstrate that R cell growth cones lacking Gek function grow to the appropriate ganglion, but frequently fail to innervate the correct target column. Further studies reveal that R cell axons lacking the activity of the small GTPase Cdc42 display similar defects, providing evidence that these proteins regulate a common set of processes. Gek is expressed in all R cells, and a detailed structure-function analysis reveals a set of regulatory domains with activities that restrict Gek function to the growth cone. Although Gek does not normally regulate layer-specific targeting, ectopic expression of Gek is sufficient to alter the targeting choices made by another R cell type, the targeting of which is normally Gek independent. Thus, specific regulation of cytoskeletal responses to targeting cues is necessary for cell type-appropriate synaptic specificity.

  15. Software Requirements Specification: Multi-scale Epidemiological and Economic Simulation and Analysis (MESA) Scenario Bank

    SciTech Connect

    Dahlgren, T L; Hazlett, S G; Slone, D M; Smith, S G

    2006-11-08

    This document builds on the discussion notes from September 21, 2006. It provides a summary of the ideas relating to the scenario bank tables and their associated requirements. Two conceptual groupings were identified for the contents requirements of the scenario bank. The first, called ProjectTemplate, shall consist of groups. The second, ProjectArchive, shall consist of groups of . The figure below illustrates the multiplicity of the associations between the different tables, with color coding used to distinguish between current MESA (brown) and USDA (light green) requirements. Scenario bank tables are shown in black with their general contents specified within the box. The metadata associated with each table is expected to include database key information as well as relevant timestamps. Each File is expected to be a file with an arbitrary format.

  16. Feasibility of using the Vero SBRT system for intracranial SRS.

    PubMed

    Burghelea, Manuela; Verellen, Dirk; Gevaert, Thierry; Depuydt, Tom; Poels, Kenneth; Simon, Viorica; De Ridder, Mark

    2014-01-01

    The Vero SBRT system was benchmarked in a planning study against the Novalis SRS system for quality of delivered dose distributions to intracranial lesions and assessing the Vero system's capacity for SRS. A total of 27 patients with one brain lesion treated on the Novalis system, with 3 mm leaf width MLC and C-arm gantry, were replanned for Vero, with a 5 mm leaf width MLC mounted on an O-ring gantry allowing rotations around both the horizontal and vertical axis. The Novalis dynamic conformal arc (DCA) planning included vertex arcs, using 90° couch rotation. These vertex arcs cannot be reproduced with Vero due to the mechanical limitations of the O-ring gantry. Alternative class solutions were investigated for the Vero. Additionally, to distinguish between the effect of MLC leaf width and different beam arrangements on dose distributions, the Vero class solutions were also applied for Novalis. In addition, the added value of noncoplanar IMRT was investigated in this study. Quality of the achieved dose distributions was expressed in the conformity index (CI) and gradient index (GI), and compared using a paired Student's t-test with statistical significance for p-values ≤ 0.05. For lesions larger than 5 cm3, no statistical significant difference in conformity was observed between Vero and Novalis, but for smaller lesions, the dose distributions showed a significantly better conformity for the Novalis (ΔCI = 13.74%, p = 0.0002) mainly due to the smaller MLC leaf width. Using IMRT on Vero reduces this conformity difference to nonsignificant levels. The cutoff for achieving a GI around 3, characterizing a sharp dose falloff outside the target volume was 4 cm3 for Novalis and 7 cm3 for Vero using DCA technique. Using noncoplanar IMRT, this threshold was reduced to 3 cm3 for the Vero system. The smaller MLC and the presence of the vertex fields allow the Novalis system to better conform the dose around the lesion and to obtain steeper dose falloff outside the lesion

  17. MIXING MODELING ANALYSIS FOR SRS SALT WASTE DISPOSITION

    SciTech Connect

    Lee, S.

    2011-01-18

    Nuclear waste at Savannah River Site (SRS) waste tanks consists of three different types of waste forms. They are the lighter salt solutions referred to as supernate, the precipitated salts as salt cake, and heavier fine solids as sludge. The sludge is settled on the tank floor. About half of the residual waste radioactivity is contained in the sludge, which is only about 8 percentage of the total waste volume. Mixing study to be evaluated here for the Salt Disposition Integration (SDI) project focuses on supernate preparations in waste tanks prior to transfer to the Salt Waste Processing Facility (SWPF) feed tank. The methods to mix and blend the contents of the SRS blend tanks were evalutaed to ensure that the contents are properly blended before they are transferred from the blend tank such as Tank 50H to the SWPF feed tank. The work consists of two principal objectives to investigate two different pumps. One objective is to identify a suitable pumping arrangement that will adequately blend/mix two miscible liquids to obtain a uniform composition in the tank with a minimum level of sludge solid particulate in suspension. The other is to estimate the elevation in the tank at which the transfer pump inlet should be located where the solid concentration of the entrained fluid remains below the acceptance criterion (0.09 wt% or 1200 mg/liter) during transfer operation to the SWPF. Tank 50H is a Waste Tank that will be used to prepare batches of salt feed for SWPF. The salt feed must be a homogeneous solution satisfying the acceptance criterion of the solids entrainment during transfer operation. The work described here consists of two modeling areas. They are the mixing modeling analysis during miscible liquid blending operation, and the flow pattern analysis during transfer operation of the blended liquid. The modeling results will provide quantitative design and operation information during the mixing/blending process and the transfer operation of the blended

  18. TECHNICAL BASIS FOR VENTILATION REQUIREMENTS IN TANK FARMS OPERATING SPECIFICATIONS DOCUMENTS

    SciTech Connect

    BERGLIN, E J

    2003-06-23

    This report provides the technical basis for high efficiency particulate air filter (HEPA) for Hanford tank farm ventilation systems (sometimes known as heating, ventilation and air conditioning [HVAC]) to support limits defined in Process Engineering Operating Specification Documents (OSDs). This technical basis included a review of older technical basis and provides clarifications, as necessary, to technical basis limit revisions or justification. This document provides an updated technical basis for tank farm ventilation systems related to Operation Specification Documents (OSDs) for double-shell tanks (DSTs), single-shell tanks (SSTs), double-contained receiver tanks (DCRTs), catch tanks, and various other miscellaneous facilities.

  19. Modified protection against Toxoplasma gondii lethal infection and brain cyst formation by vaccination with SAG2 and SRS1.

    PubMed

    Mishima, M; Xuan, X; Shioda, A; Omata, Y; Fujisaki, K; Nagasawa, H; Mikami, T

    2001-04-01

    Numerous studies have supported the importance of immunity to SAG1, the most predominant antigen of Toxoplasma tachyzoite, in protection against Toxoplasma gondii infection. Nevertheless, vaccination with SAGI provides insufficient protection when compared with that of Toxoplasma lysate (TL). In order to screen the Toxoplasma antigens for immunogenic potential shown by modified protection or induction of specific immune response after infection, recombinant antigens were prepared in Eschericha coli using DNA fragments corresponding to SAG1, SAG2, SAG3, SRS1 and P54 of T. gondii RH strain maintained in our laboratory. Each of the recombinant antigen products or a mixture of the five antigens (Mix) was used to vaccinate mice. Mice then received a lethal dose of T. gondii. Up to 25% of the mice vaccinated with SAG2, SRS1, P54 and Mix survived, whereas there were no survivors in gene 10- (negative control), SAG1- and SAG3- vaccinated groups. In all the survivors, brain cysts were not observed. Conversely, vaccination with TL almost completely protected mice in the acute phase but permitted brain cyst formation and resulted in gradual decrease of survivors to 33% during 4 months of experiments. Western blot analysis on convalescent sera showed an extensive IgG induction to a 30 kDa antigen in TL-vaccinated mice, a 22 kDa in SAG2-vaccinated mice and a 55 kDa in P54-vaccinated mice. The protection modified by boost in specific antibody is suggestive of the immunogenic potential of SAG2, SRS1 and possibly P54 against T. gondii infection.

  20. Recruitment of Mediator Complex by Cell Type and Stage-Specific Factors Required for Tissue-Specific TAF Dependent Gene Activation in an Adult Stem Cell Lineage.

    PubMed

    Lu, Chenggang; Fuller, Margaret T

    2015-12-01

    Onset of terminal differentiation in adult stem cell lineages is commonly marked by robust activation of new transcriptional programs required to make the appropriate differentiated cell type(s). In the Drosophila male germ line stem cell lineage, the switch from proliferating spermatogonia to spermatocyte is accompanied by one of the most dramatic transcriptional changes in the fly, as over 1000 new transcripts turn on in preparation for meiosis and spermatid differentiation. Here we show that function of the coactivator complex Mediator is required for activation of hundreds of new transcripts in the spermatocyte program. Mediator appears to act in a sequential hierarchy, with the testis activating Complex (tMAC), a cell type specific form of the Mip/dREAM general repressor, required to recruit Mediator subunits to the chromatin, and Mediator function required to recruit the testis TAFs (tTAFs), spermatocyte specific homologs of subunits of TFIID. Mediator, tMAC and the tTAFs co-regulate expression of a major set of spermatid differentiation genes. The Mediator subunit Med22 binds the tMAC component Topi when the two are coexpressed in S2 cells, suggesting direct recruitment. Loss of Med22 function in spermatocytes causes meiosis I maturation arrest male infertility, similar to loss of function of the tMAC subunits or the tTAFs. Our results illuminate how cell type specific versions of the Mip/dREAM complex and the general transcription machinery cooperate to drive selective gene activation during differentiation in stem cell lineages. PMID:26624996

  1. 24 CFR 200.940 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... under the HUD building product standards and certification program for sealed insulating glass units... sealed insulating glass units. (a) Applicable standards. (1) All sealed insulating glass units shall be... standard: ASTM E-774-92 Standard Specification for Sealed Insulating Glass Units. (2) This standard...

  2. 24 CFR 200.940 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... under the HUD building product standards and certification program for sealed insulating glass units... sealed insulating glass units. (a) Applicable standards. (1) All sealed insulating glass units shall be... standard: ASTM E-774-92 Standard Specification for Sealed Insulating Glass Units. (2) This standard...

  3. 24 CFR 200.940 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... under the HUD building product standards and certification program for sealed insulating glass units... sealed insulating glass units. (a) Applicable standards. (1) All sealed insulating glass units shall be... standard: ASTM E-774-92 Standard Specification for Sealed Insulating Glass Units. (2) This standard...

  4. 24 CFR 200.940 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... under the HUD building product standards and certification program for sealed insulating glass units... sealed insulating glass units. (a) Applicable standards. (1) All sealed insulating glass units shall be... standard: ASTM E-774-92 Standard Specification for Sealed Insulating Glass Units. (2) This standard...

  5. 24 CFR 200.940 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... under the HUD building product standards and certification program for sealed insulating glass units... sealed insulating glass units. (a) Applicable standards. (1) All sealed insulating glass units shall be... standard: ASTM E-774-92 Standard Specification for Sealed Insulating Glass Units. (2) This standard...

  6. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... a FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as...

  7. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as applicable),...

  8. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... a FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as...

  9. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as applicable),...

  10. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as applicable),...

  11. 49 CFR 178.320 - General requirements applicable to all DOT specification cargo tank motor vehicles.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... below atmospheric pressure. Variable specification cargo tank means a cargo tank that is constructed in... (companion flange), pipe, fitting or other attachment. Inspection pressure means the pressure used to determine leak tightness of the cargo tank when testing with pneumatic pressure. Internal self-closing...

  12. 49 CFR 180.209 - Requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... corroding components; permitted mixtures of these gases (see § 173.301(d) of this subchapter); and permitted mixtures of these gases with up to 30 percent by volume of carbon dioxide, provided the gas has a dew point... gases specifically identified in paragraph (b)(1)(ii) of this section, but currently conforms with...

  13. 49 CFR 180.205 - General requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...) For a specification 3, 3A, 3AA, 3AL, 3AX, 3AXX, 3B, 3BN, or 3T cylinder filled with gases in other... so that the cylinder is free to expand in all directions. (2) The pressure indicating device of...

  14. 49 CFR 180.205 - General requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) For a specification 3, 3A, 3AA, 3AL, 3AX, 3AXX, 3B, 3BN, or 3T cylinder filled with gases in other... so that the cylinder is free to expand in all directions. (2) The pressure indicating device of...

  15. NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae

    SciTech Connect

    Xu, Liuzhong; Ajimura, M.; Padmore, R.; Klein, C.; Kleckner, N.

    1995-12-01

    This report describes the identification of a new meiosis-specific gene of Saccharomyces cerevisiae called NDT80. DNA cloning and molecular analysis revealed that the NDT80 gene maps on the right arm of chromosome 8 and is transcribed during middle meiotic prophase. 82 refs., 6 figs., 3 tabs.

  16. 49 CFR 173.301a - Additional general requirements for shipment of specification cylinders.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... temperature of 55 °C (131 °F) that is greater than permitted. (d) Cylinder pressure at 55 °C (131 °F). The... gases in specification cylinders. (b) Authorized cylinders not marked with a service pressure. For... marked or designated, except as provided in § 173.302a(b). For certain liquefied gases, the pressure...

  17. 49 CFR 173.301a - Additional general requirements for shipment of specification cylinders.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... temperature of 55 °C (131 °F) that is greater than permitted. (d) Cylinder pressure at 55 °C (131 °F). The... gases in specification cylinders. (b) Authorized cylinders not marked with a service pressure. For... marked or designated, except as provided in § 173.302a(b). For certain liquefied gases, the pressure...

  18. 75 FR 34260 - Federal Acquisition Regulation; FAR Case 2005-040, Electronic Subcontracting Reporting System (eSRS)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-16

    ... ensure that the Federal Procurement Data System-Next Generation (FPDS-NG), eSRS, and agency contract... Acquisition Regulation; FAR Case 2005-040, Electronic Subcontracting Reporting System (eSRS) AGENCIES... Electronic Subcontracting Reporting System (eSRS), rather than Standard Form 294 - Subcontract Report...

  19. Brief Report: The Social Responsiveness Scale for Adults (SRS-A)-- Initial Results in a German Cohort

    ERIC Educational Resources Information Center

    Bolte, Sven

    2012-01-01

    The Social Responsiveness Scale (SRS) is a tool for quantitative autism assessment in children and adolescents. The SRS-A addresses social responsiveness in adulthood. Reliability and validity using the German adaptation of the SRS-A was examined in 20 adults with Autism Spectrum Disorder (ASD), 62 with other mental disorders (CLIN) and 163…

  20. 49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... missing or loose bolts, nuts, or elements that may make the tank car unsafe for transportation; (5) An... excess flow valves with threaded seats for tightness; and (7) An inspection of the required markings on... facility measures the thickness of the tank car shell, heads, sumps, protective housing (i.e., domes),...

  1. 49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... missing or loose bolts, nuts, or elements that may make the tank car unsafe for transportation; (5) An... excess flow valves with threaded seats for tightness; and (7) An inspection of the required markings on... facility measures the thickness of the tank car shell, heads, sumps, protective housing (i.e., domes),...

  2. 49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... missing or loose bolts, nuts, or elements that may make the tank car unsafe for transportation; (5) An... excess flow valves with threaded seats for tightness; and (7) An inspection of the required markings on... facility measures the thickness of the tank car shell, heads, sumps, protective housing (i.e., domes),...

  3. 46 CFR 71.65-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The principal details of... control diagram showing location and type of all required fire-screen insulation, including main fire zone and subdivisions, stairway and elevator enclosures, control space enclosures, etc., and type of...

  4. 46 CFR 71.65-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The principal details of... control diagram showing location and type of all required fire-screen insulation, including main fire zone and subdivisions, stairway and elevator enclosures, control space enclosures, etc., and type of...

  5. 46 CFR 71.65-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The principal details of... control diagram showing location and type of all required fire-screen insulation, including main fire zone and subdivisions, stairway and elevator enclosures, control space enclosures, etc., and type of...

  6. 46 CFR 71.65-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The principal details of... control diagram showing location and type of all required fire-screen insulation, including main fire zone and subdivisions, stairway and elevator enclosures, control space enclosures, etc., and type of...

  7. 40 CFR 270.18 - Specific part B information requirements for waste piles.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... complied with or detailed plans and an engineering report describing how the requirements of § 264.90(b)(2) will be met. (c) Detailed plans and an engineering report describing how the waste pile is designed and...(b) of this chapter, submit detailed plans, and engineering and hydrogeological reports,...

  8. 49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... makes the tank car unsafe for transportation; (2) An inspection of the piping, valves, fittings, and... and an inspection of the protective housings for proper securement; (5) An inspection of excess flow valves having threaded seats for tightness; and (6) An inspection of the required markings on the...

  9. 10 CFR 73.23 - Protection of Safeguards Information-Modified Handling: Specific requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... assessment taking into account such factors as trending analyses and the impacts of disclosure on licensees... CFR 73.59 are exempt from the criminal history records check and background check requirements in... National Institute of Standards and Technology (NIST) has validated as conforming to Federal...

  10. 10 CFR 73.23 - Protection of Safeguards Information-Modified Handling: Specific requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... assessment taking into account such factors as trending analyses and the impacts of disclosure on licensees... CFR 73.59 are exempt from the criminal history records check and background check requirements in... National Institute of Standards and Technology (NIST) has validated as conforming to Federal...

  11. 10 CFR 73.23 - Protection of Safeguards Information-Modified Handling: Specific requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... assessment taking into account such factors as trending analyses and the impacts of disclosure on licensees... CFR 73.59 are exempt from the criminal history records check and background check requirements in... National Institute of Standards and Technology (NIST) has validated as conforming to Federal...

  12. 10 CFR 73.23 - Protection of Safeguards Information-Modified Handling: Specific requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... assessment taking into account such factors as trending analyses and the impacts of disclosure on licensees... CFR 73.59 are exempt from the criminal history records check and background check requirements in... National Institute of Standards and Technology (NIST) has validated as conforming to Federal...

  13. 40 CFR 63.1095 - What specific requirements must I comply with?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... from waste at your facility is less than 10 Mg/yr, as determined according to 40 CFR 61.342(a), the... standards specified in 40 CFR 61.348. Comply with the requirements of 40 CFR part 61, subpart FF; with the changes in Table 2 to this subpart, and as specified in paragraphs (a)(1)(i) through (v) of this...

  14. 40 CFR 63.1095 - What specific requirements must I comply with?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... from waste at your facility is less than 10 Mg/yr, as determined according to 40 CFR 61.342(a), the... standards specified in 40 CFR 61.348. Comply with the requirements of 40 CFR part 61, subpart FF; with the changes in Table 2 to this subpart, and as specified in paragraphs (a)(1)(i) through (v) of this...

  15. 40 CFR 63.1095 - What specific requirements must I comply with?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... from waste at your facility is less than 10 Mg/yr, as determined according to 40 CFR 61.342(a), the... standards specified in 40 CFR 61.348. Comply with the requirements of 40 CFR part 61, subpart FF; with the changes in Table 2 to this subpart, and as specified in paragraphs (a)(1)(i) through (v) of this...

  16. 49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... tank car that successfully passes a periodic inspection and test must be marked as prescribed in § 180... regard to any other periodic inspection and test requirements, a tank car must have an appropriate... periodic inspection interval, test technique, and acceptance criteria for the lining or coating. The...

  17. Formal Functional Test Designs: Bridging the Gap Between Test Requirements and Test Specifications

    NASA Technical Reports Server (NTRS)

    Hops, Jonathan

    1993-01-01

    This presentation describes the testing life cycle, the purpose of the test design phase, and test design methods and gives an example application. Also included is a description of Test Representation Language (TRL), a summary of the language, and an example of an application of TRL. A sample test requirement and sample test design are included.

  18. 40 CFR 60.2660 - What site-specific documentation is required?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... recordkeeping procedures. (8) The waste management plan required under §§ 60.2620 through 60.2630. (9... Compliance Times for Commercial and Industrial Solid Waste Incineration Units that Commenced Construction On or Before November 30, 1999 Model Rule-Operator Training and Qualification § 60.2660 What...

  19. 40 CFR 60.2095 - What site-specific documentation is required?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Commercial and Industrial Solid Waste Incineration Units Operator Training and Qualification § 60.2095 What...) Procedures for receiving, handling, and charging waste. (3) Incinerator startup, shutdown, and malfunction... limits. (7) Reporting and recordkeeping procedures. (8) The waste management plan required under §§...

  20. 40 CFR 60.2095 - What site-specific documentation is required?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Commercial and Industrial Solid Waste Incineration Units Operator Training and Qualification § 60.2095 What...) Procedures for receiving, handling, and charging waste. (3) Incinerator startup, shutdown, and malfunction... limits. (7) Reporting and recordkeeping procedures. (8) The waste management plan required under §§...

  1. 40 CFR 60.2660 - What site-specific documentation is required?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Compliance Times for Commercial and Industrial Solid Waste Incineration Units Model Rule-Operator Training... subpart. (2) Procedures for receiving, handling, and charging waste. (3) Incinerator startup, shutdown... operating limits. (7) Reporting and recordkeeping procedures. (8) The waste management plan required...

  2. 49 CFR 171.23 - Requirements for specific materials and packagings transported under the ICAO Technical...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... to the following requirements: (i) The words “Poison-Inhalation Hazard” or “Toxic-Inhalation Hazard... description. The word “Poison” or “Toxic” or the phrase “Poison-Inhalation Hazard” or “Toxic-Inhalation Hazard... Transport Canada TDG Regulations, the package must be labeled or placarded with POISON INHALATION HAZARD...

  3. 24 CFR 200.945 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... under the HUD building product standards and certification program for carpet. 200.945 Section 200.945... requirements under the HUD building product standards and certification program for carpet. (a) Applicable standards. (1) All carpet shall be designed, manufactured, and tested in compliance with the...

  4. 24 CFR 200.945 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... under the HUD building product standards and certification program for carpet. 200.945 Section 200.945... requirements under the HUD building product standards and certification program for carpet. (a) Applicable standards. (1) All carpet shall be designed, manufactured, and tested in compliance with the...

  5. 24 CFR 200.945 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... under the HUD building product standards and certification program for carpet. 200.945 Section 200.945... requirements under the HUD building product standards and certification program for carpet. (a) Applicable standards. (1) All carpet shall be designed, manufactured, and tested in compliance with the...

  6. 24 CFR 200.945 - Supplementary specific requirements under the HUD building product standards and certification...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... under the HUD building product standards and certification program for carpet. 200.945 Section 200.945... requirements under the HUD building product standards and certification program for carpet. (a) Applicable standards. (1) All carpet shall be designed, manufactured, and tested in compliance with the...

  7. 49 CFR 180.209 - Requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... mixtures of these gases (see § 173.301(d) of this subchapter); and permitted mixtures of these gases with up to 30 percent by volume of carbon dioxide, provided the gas has a dew point at or below minus (52... last required requalification, a cylinder has not been used exclusively for the gases...

  8. 49 CFR 180.209 - Requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... mixtures of these gases (see § 173.301(d) of this subchapter); and permitted mixtures of these gases with up to 30 percent by volume of carbon dioxide, provided the gas has a dew point at or below minus (52... last required requalification, a cylinder has not been used exclusively for the gases...

  9. 49 CFR 180.209 - Requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... mixtures of these gases (see § 173.301(d) of this subchapter); and permitted mixtures of these gases with up to 30 percent by volume of carbon dioxide, provided the gas has a dew point at or below minus (52... last required requalification, a cylinder has not been used exclusively for the gases...

  10. 49 CFR 180.209 - Requirements for requalification of specification cylinders.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... mixtures of these gases (see § 173.301(d) of this subchapter); and permitted mixtures of these gases with up to 30 percent by volume of carbon dioxide, provided the gas has a dew point at or below minus (52... last required requalification, a cylinder has not been used exclusively for the gases...

  11. 46 CFR 127.110 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...-transfer systems for fixed independent or portable tanks. (e) Electrical engineering. (1) For each vessel... engineering. (1) Piping diagrams of each Class I systems. (2) Piping diagrams of the following Class II... or more gross tons, the plans required by § 110.25 of this chapter must be submitted. (f)...

  12. 46 CFR 127.110 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...-transfer systems for fixed independent or portable tanks. (e) Electrical engineering. (1) For each vessel... engineering. (1) Piping diagrams of each Class I systems. (2) Piping diagrams of the following Class II... or more gross tons, the plans required by § 110.25 of this chapter must be submitted. (f)...

  13. 46 CFR 189.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... boilers. (9) * Arrangement of ports, doors, and airports in shell plating. (10) * Hatch coamings and covers in weather and watertight decks. (11) * Details of hinged subdivision watertight doors and... stability. Plans required by part 170 of this chapter. (d) Fire control. (1) General arrangement...

  14. 46 CFR 189.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... boilers. (9) * Arrangement of ports, doors, and airports in shell plating. (10) * Hatch coamings and covers in weather and watertight decks. (11) * Details of hinged subdivision watertight doors and... stability. Plans required by part 170 of this chapter. (d) Fire control. (1) General arrangement...

  15. 40 CFR 270.18 - Specific part B information requirements for waste piles.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... complied with; (h) A description of how hazardous waste residues and contaminated materials will be removed... requirements for waste piles. 270.18 Section 270.18 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) EPA ADMINISTERED PERMIT PROGRAMS: THE HAZARDOUS WASTE PERMIT...

  16. 40 CFR 63.1095 - What specific requirements must I comply with?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... from waste at your facility is less than 10 Mg/yr, as determined according to 40 CFR 61.342(a), the... standards specified in 40 CFR 61.348. Comply with the requirements of 40 CFR part 61, subpart FF; with the.... (i) Determine the butadiene concentration of the waste stream according to 40 CFR...

  17. 40 CFR 63.1095 - What specific requirements must I comply with?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... from waste at your facility is less than 10 Mg/yr, as determined according to 40 CFR 61.342(a), the... standards specified in 40 CFR 61.348. Comply with the requirements of 40 CFR part 61, subpart FF; with the.... (i) Determine the butadiene concentration of the waste stream according to 40 CFR...

  18. 46 CFR 91.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) *Stem, Stern Frame, and Rudder. (5) *Structural Deck Plans for Strength Decks. (6) *Pillars and Girders..., and sprinkling systems. (e) Marine engineering. For plans required for marine engineering equipment and systems, see subchapter F (Marine Engineering) of this chapter. (f) Electrical engineering....

  19. 46 CFR 189.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... crewmembers and scientific personnel. (i) Magazines and magazine vans. (1) All plans relating to the arrangement, construction, ventilation, and fire protection system for magazines and magazine vans. (The plans required for magazines and magazine vans to be installed or carried on a vessel after the vessel is...

  20. 33 CFR 155.5035 - Nontank vessel response plan requirements: Specific content.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .../78 (as set forth in 33 CFR 151.26 and 33 CFR part 153); and (ii) Any applicable State; (2... operating exclusively on the inland areas of the United States, the GSA must also separately list the... pollution incident as required 33 CFR 151.26(b)(3)(iii). This information must also clearly specify who...

  1. Specification of requirements for the virtual environment for reactor applications simulation environment

    SciTech Connect

    Hess, S. M.; Pytel, M.

    2012-07-01

    In 2010, the United States Dept. of Energy initiated a research and development effort to develop modern modeling and simulation methods that could utilize high performance computing capabilities to address issues important to nuclear power plant operation, safety and sustainability. To respond to this need, a consortium of national laboratories, academic institutions and industry partners (the Consortium for Advanced Simulation of Light Water Reactors - CASL) was formed to develop an integrated Virtual Environment for Reactor Applications (VERA) modeling and simulation capability. A critical element for the success of the CASL research and development effort was the development of an integrated set of overarching requirements that provides guidance in the planning, development, and management of the VERA modeling and simulation software. These requirements also provide a mechanism from which the needs of a broad array of external CASL stakeholders (e.g. reactor / fuel vendors, plant owner / operators, regulatory personnel, etc.) can be identified and integrated into the VERA development plans. This paper presents an overview of the initial set of requirements contained within the VERA Requirements Document (VRD) that currently is being used to govern development of the VERA software within the CASL program. The complex interdisciplinary nature of these requirements together with a multi-physics coupling approach to realize a core simulator capability pose a challenge to how the VRD should be derived and subsequently revised to accommodate the needs of different stakeholders. Thus, the VRD is viewed as an evolving document that will be updated periodically to reflect the changing needs of identified CASL stakeholders and lessons learned during the progress of the CASL modeling and simulation program. (authors)

  2. Improving the measurement of health-related quality of life in adolescent with idiopathic scoliosis: the SRS-7, a Rasch-developed short form of the SRS-22 questionnaire.

    PubMed

    Caronni, Antonio; Zaina, Fabio; Negrini, Stefano

    2014-04-01

    Scoliosis Research Society-22 (SRS-22) questionnaire was developed to evaluate health-related quality of life (HRQL) in adolescent idiopathic scoliosis (AIS) patients. Rasch analysis (RA) is a statistical procedure which turns questionnaire ordinal scores into interval measures. Measures from Rasch-compatible questionnaires can be used, similar to body temperature or blood pressure, to quantify disease severity progression and treatment efficacy. Purpose of the current work is to present Rasch analysis (RA) of the SRS-22 questionnaire and to develop an SRS-22 Rasch-approved short form. 300 SRS-22 were randomly collected from 2447 consecutive IS adolescents at their first evaluation (229 females; 13.9 ± 1.9 years; 26.9 ± 14.7 Cobb°) in a scoliosis outpatient clinic. RA showed both disordered thresholds and overall misfit of the SRS-22. Sixteen items were re-scored and two misfitting items (6 and 14) removed to obtain a Rasch-compatible questionnaire. Participants HRQL measured too high with the rearranged questionnaire, indicating a severe SRS-22 ceiling effect. RA also highlighted SRS-22 multidimensionality, with pain/function not merging with self-image/mental health items. Item 3 showed differential item functioning (DIF) for both curve and hump amplitude. A 7-item questionnaire (SRS-7) was prepared by selecting single items from the original SRS-22. SRS-7 showed fit to the model, unidimensionality and no DIF. Compared with the SRS-22, the short form scale shows better targeting of the participants' population. RA shows that SRS-22 has poor clinimetric properties; moreover, when used with AIS at first evaluation, SRS-22 is affected by a severe ceiling effect. SRS-7, an SRS-22 7-item short form questionnaire, provides an HRQL interval measure better tailored to these participants.

  3. Hemogenic endothelial cell specification requires c-kit, notch signaling, and p27-mediated cell-cycle control

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Delineating the mechanism or mechanisms that regulate the specification of hemogenic endothelial cells from primordial endothelium is critical for optimizing their derivation from human stem cells for clinical therapies. We previously determined that retinoic acid (RA) is required for hemogenic spec...

  4. Nuclear Technology. Course 26: Nondestructive Examination (NDE) Techniques I. Module 26-7, Radiographic Specifications and Code Requirements.

    ERIC Educational Resources Information Center

    Pelton, Rick; Espy, John

    This seventh in a series of seven modules for a course titled Nondestructive Examination (NDE) Techniques I describes radiographic specifications and code requirements. The module follows a typical format that includes the following sections: (1) introduction, (2) module prerequisites, (3) objectives, (4) notes to instructor/student, (5) subject…

  5. 40 CFR 152.95 - Citation of all studies in the Agency's files pertinent to a specific data requirement.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Citation of all studies in the Agency's files pertinent to a specific data requirement. 152.95 Section 152.95 Protection of Environment... PROCEDURES Procedures To Ensure Protection of Data Submitters' Rights § 152.95 Citation of all studies in...

  6. 40 CFR Table C-1 to Subpart C of... - Test Concentration Ranges, Number of Measurements Required, and Maximum Discrepancy Specifications

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 6 2013-07-01 2013-07-01 false Test Concentration Ranges, Number of Measurements Required, and Maximum Discrepancy Specifications C Table C-1 to Subpart C of Part 53 Protection of... Reference Methods Pt. 53, Subpt. C, Table C-1 Table C-1 to Subpart C of Part 53—Test Concentration...

  7. 40 CFR Table C-1 to Subpart C of... - Test Concentration Ranges, Number of Measurements Required, and Maximum Discrepancy Specifications

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 6 2014-07-01 2014-07-01 false Test Concentration Ranges, Number of Measurements Required, and Maximum Discrepancy Specifications C Table C-1 to Subpart C of Part 53 Protection of... Reference Methods Pt. 53, Subpt. C, Table C-1 Table C-1 to Subpart C of Part 53—Test Concentration...

  8. 40 CFR Table C-1 to Subpart C of... - Test Concentration Ranges, Number of Measurements Required, and Maximum Discrepancy Specifications

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 6 2012-07-01 2012-07-01 false Test Concentration Ranges, Number of Measurements Required, and Maximum Discrepancy Specifications C Table C-1 to Subpart C of Part 53 Protection of... Reference Methods Pt. 53, Subpt. C, Table C-1 Table C-1 to Subpart C of Part 53—Test Concentration...

  9. 40 CFR Table C-1 to Subpart C of... - Test Concentration Ranges, Number of Measurements Required, and Maximum Discrepancy Specifications

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 5 2011-07-01 2011-07-01 false Test Concentration Ranges, Number of Measurements Required, and Maximum Discrepancy Specifications C Table C-1 to Subpart C of Part 53 Protection of... Reference Methods Pt. 53, Subpt. C, Table C-1 Table C-1 to Subpart C of Part 53—Test Concentration...

  10. 40 CFR 152.95 - Citation of all studies in the Agency's files pertinent to a specific data requirement.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Citation of all studies in the Agency's files pertinent to a specific data requirement. 152.95 Section 152.95 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PESTICIDE REGISTRATION AND...

  11. 33 CFR 154.1240 - Specific requirements for animal fats and vegetable oils facilities that could reasonably be...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... fats and vegetable oils facilities that could reasonably be expected to cause substantial harm to the... SECURITY (CONTINUED) POLLUTION FACILITIES TRANSFERRING OIL OR HAZARDOUS MATERIAL IN BULK Response Plans for Animal Fats and Vegetable Oils Facilities § 154.1240 Specific requirements for animal fats and...

  12. 33 CFR 154.1240 - Specific requirements for animal fats and vegetable oils facilities that could reasonably be...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... fats and vegetable oils facilities that could reasonably be expected to cause substantial harm to the... SECURITY (CONTINUED) POLLUTION FACILITIES TRANSFERRING OIL OR HAZARDOUS MATERIAL IN BULK Response Plans for Animal Fats and Vegetable Oils Facilities § 154.1240 Specific requirements for animal fats and...

  13. 33 CFR 154.1240 - Specific requirements for animal fats and vegetable oils facilities that could reasonably be...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... fats and vegetable oils facilities that could reasonably be expected to cause substantial harm to the... SECURITY (CONTINUED) POLLUTION FACILITIES TRANSFERRING OIL OR HAZARDOUS MATERIAL IN BULK Response Plans for Animal Fats and Vegetable Oils Facilities § 154.1240 Specific requirements for animal fats and...

  14. 33 CFR 154.1240 - Specific requirements for animal fats and vegetable oils facilities that could reasonably be...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... fats and vegetable oils facilities that could reasonably be expected to cause substantial harm to the... SECURITY (CONTINUED) POLLUTION FACILITIES TRANSFERRING OIL OR HAZARDOUS MATERIAL IN BULK Response Plans for Animal Fats and Vegetable Oils Facilities § 154.1240 Specific requirements for animal fats and...

  15. 33 CFR 154.1240 - Specific requirements for animal fats and vegetable oils facilities that could reasonably be...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... fats and vegetable oils facilities that could reasonably be expected to cause substantial harm to the... SECURITY (CONTINUED) POLLUTION FACILITIES TRANSFERRING OIL OR HAZARDOUS MATERIAL IN BULK Response Plans for Animal Fats and Vegetable Oils Facilities § 154.1240 Specific requirements for animal fats and...

  16. 10 CFR 33.15 - Requirements for the issuance of a Type C specific license of broad scope.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... OF BROAD SCOPE FOR BYPRODUCT MATERIAL Specific Licenses of Broad Scope § 33.15 Requirements for the... this chapter; and (b) The applicant submits a statement that byproduct material will be used only by... and forms of byproduct material to be used; and (c) The applicant has established...

  17. 10 CFR 33.15 - Requirements for the issuance of a Type C specific license of broad scope.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... OF BROAD SCOPE FOR BYPRODUCT MATERIAL Specific Licenses of Broad Scope § 33.15 Requirements for the... this chapter; and (b) The applicant submits a statement that byproduct material will be used only by... and forms of byproduct material to be used; and (c) The applicant has established...

  18. 49 CFR 179.401 - Individual specification requirements applicable to inner tanks for cryogenic liquid tank car tanks.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Individual specification requirements applicable to inner tanks for cryogenic liquid tank car tanks. 179.401 Section 179.401 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS...

  19. 40 CFR 761.375 - Specific requirements for surfaces coated or covered with dust, dirt, grime, grease, or another...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Specific requirements for surfaces coated or covered with dust, dirt, grime, grease, or another absorbent material. 761.375 Section 761.375 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) TOXIC SUBSTANCES CONTROL...

  20. 40 CFR 761.375 - Specific requirements for surfaces coated or covered with dust, dirt, grime, grease, or another...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Specific requirements for surfaces coated or covered with dust, dirt, grime, grease, or another absorbent material. 761.375 Section 761.375 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) TOXIC SUBSTANCES CONTROL...