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Sample records for rock mechanics activities

  1. Rock mechanics research awards

    NASA Astrophysics Data System (ADS)

    Wagner, John E.

    The U.S. National Committee for Rock Mechanics, at its June 1983 annual meeting, adopted three actions to enhance the competition and public awareness of its annual awards program for rock mechanics papers. It will issue a call for nominations of outstanding papers; it will request participating societies to announce the names of award winners and the titles of papers, and it will publish an abstract of the winning papers in the proceedings of the annual U.S. Rock Mechanics Symposium in the year following the awards.The competition is open to papers, by U.S residents or students in a U.S. school, published in an English language publication normally available in the United States. The following authors and papers are the 1983 award winners:

  2. Mechanism of lead immobilization by oxalic acid-activated phosphate rocks.

    PubMed

    Jiang, Guanjie; Liu, Yonghong; Huang, Li; Fu, Qingling; Deng, Youjun; Hu, Hongqing

    2012-01-01

    Lead (Pb) chemical fixation is an important environmental aspect for human health. Phosphate rocks (PRs) were utilized as an adsorbent to remove Pb from aqueous solution. Raw PRs and oxalic acid-activated PRs (APRs) were used to investigate the effect of chemical modification on the Pb-binding capacity in the pH range 2.0-5.0. The Pb adsorption rate of all treatments above pH 3.0 reached 90%. The Pb binding on PRs and APRs was pH-independent, except at pH 2.0 in activated treatments. The X-ray diffraction analysis confirmed that the raw PRs formed cerussite after reacting with the Pb solution, whereas the APRs formed pyromorphite. The Fourier Transform Infrared spectroscopy analysis indicated that carbonate (CO3(2-)) in raw PRs and phosphate (PO4(3)) groups in APRs played an important role in the Pb-binding process. After adsorption, anomalous block-shaped particles were observed by scanning electron microscopy with energy dispersive spectroscopy. The X-ray photoelectron spectroscopy data further indicated that both chemical and physical reactions occurred during the adsorption process according to the binding energy. Because of lower solubility of pyromorphite compared to cerussite, the APRs are more effective in immobilizing Pb than that of PRs.

  3. Ultra-fine grinding and mechanical activation of mine waste rock using a high-speed stirred mill for mineral carbonation

    NASA Astrophysics Data System (ADS)

    Li, Jia-jie; Hitch, Michael

    2015-10-01

    CO2 sequestration by mineral carbonation can permanently store CO2 and mitigate climate change. However, the cost and reaction rate of mineral carbonation must be balanced to be viable for industrial applications. In this study, it was attempted to reduce the carbonation costs by using mine waste rock as a feed stock and to enhance the reaction rate using wet mechanical activation as a pre-treatment method. Slurry rheological properties, particle size distribution, specific surface area, crystallinity, and CO2 sequestration reaction efficiency of the initial and mechanically activated mine waste rock and olivine were characterized. The results show that serpentine acts as a catalyst, increasing the slurry yield stress, assisting new surface formation, and hindering the size reduction and structure amorphization. Mechanically activated mine waste rock exhibits a higher carbonation conversion than olivine with equal specific milling energy input. The use of a high-speed stirred mill may render the mineral carbonation suitable for mining industrial practice.

  4. Rock mechanics for hard rock nuclear waste repositories

    SciTech Connect

    Heuze, F.E.

    1981-09-01

    The mined geologic burial of high level nuclear waste is now the favored option for disposal. The US National Waste Terminal Storage Program designed to achieve this disposal includes an extensive rock mechanics component related to the design of the wastes repositories. The plan currently considers five candidate rock types. This paper deals with the three hard rocks among them: basalt, granite, and tuff. Their behavior is governed by geological discontinuities. Salt and shale, which exhibit behavior closer to that of a continuum, are not considered here. This paper discusses both the generic rock mechanics R and D, which are required for repository design, as well as examples of projects related to hard rock waste storage. The examples include programs in basalt (Hanford/Washington), in granitic rocks (Climax/Nevada Test Site, Idaho Springs/Colorado, Pinawa/Canada, Oracle/Arizona, and Stripa/Sweden), and in tuff (Nevada Test Site).

  5. Rock mechanics contributions from defense programs

    SciTech Connect

    Heuze, F.E.

    1992-02-01

    An attempt is made at illustrating the many contributions to rock mechanics from US defense programs, over the past 30-plus years. Large advances have been achieved in the technology-base area covering instrumentation, material properties, physical modeling, constitutive relations and numerical simulations. In the applications field, much progress has been made in understanding and being able to predict rock mass behavior related to underground explosions, cratering, projectile penetration, and defense nuclear waste storage. All these activities stand on their own merit as benefits to national security. But their impact is even broader, because they have found widespread applications in the non-defense sector; to name a few: the prediction of the response of underground structures to major earthquakes, the physics of the earth`s interior at great depths, instrumentation for monitoring mine blasting, thermo-mechanical instrumentation useful for civilian nuclear waste repositories, dynamic properties of earthquake faults, and transient large-strain numerical modeling of geological processes, such as diapirism. There is not pretense that this summary is exhaustive. It is meant to highlight success stories representative of DOE and DOD geotechnical activities, and to point to remaining challenges.

  6. Phosphine from rocks: mechanically driven phosphate reduction?

    PubMed

    Glindemann, Dietmar; Edwards, Marc; Morgenstern, Peter

    2005-11-01

    Natural rock and mineral samples released trace amounts of phosphine during dissolution in mineral acid. An order of magnitude more phosphine (average 1982 ng PH3 kg rock and maximum 6673 ng PH3/kg rock) is released from pulverized rock samples (basalt, gneiss, granite, clay, quartzitic pebbles, or marble). Phosphine was correlated to hardness and mechanical pulverization energy of the rocks. The yield of PH3 ranged from 0 to 0.01% of the total P content of the dissolved rock. Strong circumstantial evidence was gathered for reduction of phosphate in the rock via mechanochemical or "tribochemical" weathering at quartz and calcite/marble inclusions. Artificial reproduction of this mechanism by rubbing quartz rods coated with apatite-phosphate to the point of visible triboluminescence, led to detection of more than 70 000 ng/kg PH3 in the apatite. This reaction pathway may be considered a mechano-chemical analogue of phosphate reduction from lightning or electrical discharges and may contribute to phosphine production via tectonic forces and processing of rocks.

  7. Mechanism of Rock Burst Occurrence in Specially Thick Coal Seam with Rock Parting

    NASA Astrophysics Data System (ADS)

    Wang, Jian-chao; Jiang, Fu-xing; Meng, Xiang-jun; Wang, Xu-you; Zhu, Si-tao; Feng, Yu

    2016-05-01

    Specially thick coal seam with complex construction, such as rock parting and alternative soft and hard coal, is called specially thick coal seam with rock parting (STCSRP), which easily leads to rock burst during mining. Based on the stress distribution of rock parting zone, this study investigated the mechanism, engineering discriminant conditions, prevention methods, and risk evaluation method of rock burst occurrence in STCSRP through setting up a mechanical model. The main conclusions of this study are as follows. (1) When the mining face moves closer to the rock parting zone, the original non-uniform stress of the rock parting zone and the advancing stress of the mining face are combined to intensify gradually the shearing action of coal near the mining face. When the shearing action reaches a certain degree, rock burst easily occurs near the mining face. (2) Rock burst occurrence in STCSRP is positively associated with mining depth, advancing stress concentration factor of the mining face, thickness of rock parting, bursting liability of coal, thickness ratio of rock parting to coal seam, and difference of elastic modulus between rock parting and coal, whereas negatively associated with shear strength. (3) Technologies of large-diameter drilling, coal seam water injection, and deep hole blasting can reduce advancing stress concentration factor, thickness of rock parting, and difference of elastic modulus between rock parting and coal to lower the risk of rock burst in STCSRP. (4) The research result was applied to evaluate and control the risk of rock burst occurrence in STCSRP.

  8. Laboratory rock mechanics testing manual. Public draft

    SciTech Connect

    Shuri, F S; Cooper, J D; Hamill, M L

    1981-10-01

    Standardized laboratory rock mechanics testing procedures have been prepared for use in the National Terminal Waste Storage Program. The procedures emphasize equipment performance specifications, documentation and reporting, and Quality Assurance acceptance criteria. Sufficient theoretical background is included to allow the user to perform the necessary data reduction. These procedures incorporate existing standards when possible, otherwise they represent the current state-of-the-art. Maximum flexibility in equipment design has been incorporated to allow use of this manual by existing groups and to encourage future improvements.

  9. Physical vs. Mathematical Models in Rock Mechanics

    NASA Astrophysics Data System (ADS)

    Morozov, I. B.; Deng, W.

    2013-12-01

    One of the less noted challenges in understanding the mechanical behavior of rocks at both in situ and lab conditions is the character of theoretical approaches being used. Currently, the emphasis is made on spatial averaging theories (homogenization and numerical models of microstructure), empirical models for temporal behavior (material memory, compliance functions and complex moduli), and mathematical transforms (Laplace and Fourier) used to infer the Q-factors and 'relaxation mechanisms'. In geophysical applications, we have to rely on such approaches for very broad spatial and temporal scales which are not available in experiments. However, the above models often make insufficient use of physics and utilize, for example, the simplified 'correspondence principle' instead of the laws of viscosity and friction. As a result, the commonly-used time- and frequency dependent (visco)elastic moduli represent apparent properties related to the measurement procedures and not necessarily to material properties. Predictions made from such models may therefore be inaccurate or incorrect when extrapolated beyond the lab scales. To overcome the above challenge, we need to utilize the methods of micro- and macroscopic mechanics and thermodynamics known in theoretical physics. This description is rigorous and accurate, uses only partial differential equations, and allows straightforward numerical implementations. One important observation from the physical approach is that the analysis should always be done for the specific geometry and parameters of the experiment. Here, we illustrate these methods on axial deformations of a cylindrical rock sample in the lab. A uniform, isotropic elastic rock with a thermoelastic effect is considered in four types of experiments: 1) axial extension with free transverse boundary, 2) pure axial extension with constrained transverse boundary, 3) pure bulk expansion, and 4) axial loading harmonically varying with time. In each of these cases, an

  10. High-pressure mechanical instability in rocks

    USGS Publications Warehouse

    Byerlee, J.D.; Brace, W.F.

    1969-01-01

    At a confining pressure of a few kilobars, deformation of many sedimentary rocks, altered mafic rocks, porous volcanic rocks, and sand is ductile, in that instabilities leading to audible elastic shocks are absent. At pressures of 7 to 10 kilobars, however, unstable faulting and stick-slip in certain of these rocks was observed. This high pressure-low temperature instability might be responsible for earthquakes in deeply buried sedimentary or volcanic sequences.

  11. High-pressure mechanical instability in rocks.

    PubMed

    Byerlee, J D; Brace, W F

    1969-05-01

    At a confining pressure of a few kilobars, deformation of many sedimentary rocks, altered mafic rocks, porous volcanic rocks, and sand is ductile, in that instabilities leading to audible elastic shocks are absent. At pressures of 7 to 10 kilobars, however, unstable faulting and stick-slip in certain of these rocks was observed. This high pressure-low temperature instability might be responsible for earthquakes in deeply buried sedimentary or volcanic sequences.

  12. Panel discussion on rock mechanics issues in repository design

    SciTech Connect

    Bieniawski, Z.T.; Kim, K.S.; Nataraja, M.

    1996-04-01

    The panel discussion was introduced by Mr. Z.T.(Richard) Bieniawski and then continued with five additional speakers. The topics covered in the discussion included rock mechanics pertaining to the design of underground facilities for the disposal of radioactive wastes and the safety of such facilities. The speakers included: Mr. Kun-Soo Kim who is a specialist in the area of rock mechanics testing during the Basalt Waste Isolation Project; Dr. Mysore Nataraja who is the senior project manager with the NRC; Dr. Michael Voegele who is the project manager for Science Applications International Corporation (SAIC) on the Yucca Mountain Project; Dr. Edward Cording who is a member of the Nuclear Waste Technical Review Board; and Dr. Hemendra Kalia who is employed by Los Alamos National Laboratory and coordinates various activities of testing programs at the Yucca Mountain Site.

  13. Influence of sedimentary environments on mechanical properties of clastic rocks

    NASA Astrophysics Data System (ADS)

    Meng, Zhaoping; Zhang, Jincai; Peng, Suping

    2006-10-01

    The sedimentary environments are the intrinsic factor controlling the mechanical properties of clastic rocks. Examining the relationship between rock sedimentary environments and rock mechanical properties gives a better understanding of rock deformation and failure mechanisms. In this study, more than 55 samples in coal measures were taken from seven different lithologic formations in eastern China. Using the optical microscope the sedimentary characteristics, such as components of clastic rocks and sizes of clastic grains were quantitatively tested and analyzed. The corresponding mechanical parameters were tested using the servo-controlled testing system. Different lithologic attributes in the sedimentary rocks sampled different stress-strain behaviors and failure characteristics under different confining pressures, mainly due to different compositions and textures. Results demonstrate that clastic rocks have the linear best-fit for Mohr-Coulomb failure criterion. The elastic moduli in clastic rocks are highly dependent upon confining pressures, unlike hard rocks. The envelope lines of the mechanical properties versus the contents of quartz, detritus of the grain diameter of more than 0.03 mm, and grain size in clastic rocks are given. The compressive strength or elastic modulus and the grain diameter have a non-monotonic relation and demonstrate the “grain-diameter softening” effect.

  14. The Weathering of Rocks: Three Activities.

    ERIC Educational Resources Information Center

    McLure, John W.

    1991-01-01

    Integrates science and social studies in several activities that study weathering caused by the freezing and thawing of rocks, wind erosion, and the effects of weathering on tombstones. Cites the possibility of these activities leading to an interdisciplinary exploration of pollution, customs, and populations. (MCO)

  15. Mechanical and acoustic properties of weakly cemented granular rocks

    SciTech Connect

    Nakagawa, S.; Myer, L.R.

    2001-05-09

    This paper presents the results of laboratory measurements on the mechanical and acoustic properties of weakly cemented granular rock. Artificial rock samples were fabricated by cementing sand and glass beads with sodium silicate binder. During uniaxial compression tests, the rock samples showed stress-strain behavior which was more similar to that of soils than competent rocks, exhibiting large permanent deformations with frictional slip. The mechanical behavior of the samples approached that of competent rocks as the amount of binder was increased. For very weak samples, acoustic waves propagating in these rocks showed very low velocities of less than 1000 m/sec for compressional waves. A borehole made within this weakly cemented rock exhibited a unique mode of failure that is called ''anti-KI mode fracture'' in this paper. The effect of cementation, grain type, and boundary conditions on this mode of failure was also examined experimentally.

  16. Rock mechanics. Superplastic nanofibrous slip zones control seismogenic fault friction.

    PubMed

    Verberne, Berend A; Plümper, Oliver; de Winter, D A Matthijs; Spiers, Christopher J

    2014-12-12

    Understanding the internal mechanisms controlling fault friction is crucial for understanding seismogenic slip on active faults. Displacement in such fault zones is frequently localized on highly reflective (mirrorlike) slip surfaces, coated with thin films of nanogranular fault rock. We show that mirror-slip surfaces developed in experimentally simulated calcite faults consist of aligned nanogranular chains or fibers that are ductile at room conditions. These microstructures and associated frictional data suggest a fault-slip mechanism resembling classical Ashby-Verrall superplasticity, capable of producing unstable fault slip. Diffusive mass transfer in nanocrystalline calcite gouge is shown to be fast enough for this mechanism to control seismogenesis in limestone terrains. With nanogranular fault surfaces becoming increasingly recognized in crustal faults, the proposed mechanism may be generally relevant to crustal seismogenesis.

  17. Mechanical stimulation of cyclic tensile strain induces reduction of pluripotent related gene expressions via activation of Rho/ROCK and subsequent decreasing of AKT phosphorylation in human induced pluripotent stem cells

    SciTech Connect

    Teramura, Takeshi; Takehara, Toshiyuki; Onodera, Yuta; Nakagawa, Koichi; Hamanishi, Chiaki; Fukuda, Kanji

    2012-01-13

    Highlights: Black-Right-Pointing-Pointer Mechanical stimulation is an important factor for regulation of stem cell fate. Black-Right-Pointing-Pointer Cyclic stretch to human induced pluripotent stem cells activated small GTPase Rho. Black-Right-Pointing-Pointer Rho-kinase activation attenuated pluripotency via inhibition of AKT activation. Black-Right-Pointing-Pointer This reaction could be reproduced only by transfection of dominant active Rho. Black-Right-Pointing-Pointer Rho/ROCK are important molecules in mechanotransduction and control of stemness. -- Abstract: Mechanical stimulation has been shown to regulate the proliferation and differentiation of stem cells. However, the effects of the mechanical stress on the stemness or related molecular mechanisms have not been well determined. Pluripotent stem cells such as embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are used as good materials for cell transplantation therapy and research of mammalian development, since they can self-renew infinitely and differentiate into various cell lineages. Here we demonstrated that the mechanical stimulation to human iPS cells altered alignment of actin fibers and expressions of the pluripotent related genes Nanog, POU5f1 and Sox2. In the mechanically stimulated iPS cells, small GTPase Rho was activated and interestingly, AKT phosphorylation was decreased. Inhibition of Rho-associated kinase ROCK recovered the AKT phosphorylation and the gene expressions. These results clearly suggested that the Rho/ROCK is a potent primary effector of mechanical stress in the pluripotent stem cells and it participates to pluripotency-related signaling cascades as an upper stream regulator.

  18. Significance of grain sliding mechanisms for ductile deformation of rocks

    NASA Astrophysics Data System (ADS)

    Dimanov, A.; Bourcier, M.; Gaye, A.; Héripré, E.; Bornert, M.; Raphanel, J.; Ludwig, W.

    2013-12-01

    Ductile shear zones at depth present polyphase and heterogeneous rocks and multi-scale strain localization patterns. Most strain concentrates in ultramylonitic layers, which exhibit microstructural signatures of several concomitant deformation mechanisms. The latter are either active in volume (dislocation creep), or in the vicinity and along interfaces (grain sliding and solution mass transfer). Because their chronology of appearance and interactions are unclear, inference of the overall rheology seems illusory. We have therefore characterized over a decade the rheology of synthetic lower crustal materials with different compositions and fluid contents, and for various microstructures. Non-Newtonian flow clearly related to dominant dislocation creep. Conversely, Newtonian behavior involved grain sliding mechanisms, but crystal plasticity could be identified as well. In order to clarify the respective roles of these mechanisms we underwent a multi-scale investigation of the ductile deformation of rock analog synthetic halite with controlled microstructures. The mechanical tests were combined with in-situ optical microscopy, scanning electron microscopy and X ray computed tomography, allowing for digital image correlation (DIC) techniques and retrieval of full strain field. Crystal plasticity dominated, as evidenced by physical slip lines and DIC computed slip bands. Crystal orientation mapping allowed to identify strongly active easy glide {110} <110> systems. But, all other slip systems were observed as well, and especially near interfaces, where their activity is necessary to accommodate for the plastic strain incompatibilities between neighboring grains. We also evidenced grain boundary sliding (GBS), which clearly occurred as a secondary, but necessary, accommodation mechanism. The DIC technique allowed the quantification of the relative contribution of each mechanism. The amount of GBS clearly increased with decreasing grain size. Finite element (FE) modeling

  19. Rock Mechanical Properties from Logs Petrophysics : Concepts and Results

    NASA Astrophysics Data System (ADS)

    Gaillot, Philippe; Crawford, Brian; Alramahi, Bashar; Karner, Steve

    2010-05-01

    The objective of the "geomechanics from logs" (GML) research project is to develop model-driven predictive software for determining rock mechanical properties (specifically rock strength, compressibility and fracability) from other, more easily measured, rock properties (e.g. lithology, porosity, clay volume, velocity) routinely derived from nuclear, resistivity and acoustic logging tools. To this end, geomechanics from logs seeks to increase fundamental understanding of the primary geologic controls on rock mechanical properties and to translate this new insight into novel predictive tools. In detail, GML predictors rely on (i) the generation of relational rock mechanical properties databases incorporating QC'd core-based laboratory measurements (both in-house and high-precision published data); (ii) the use of established rock physics models (e.g. friable sand, contact cement models) to investigate theoretical relationships between geologic processes, reservoir environment, rock microstructure and elastic, bulk and transport petrophysical attributes/properties; (iii) the subdivision of database rocks into generic lithotypes (e.g. sand, shaly sand, sandy shale, shale) with common petrophysical attributes/properties; (iv) the use of multivariate statistics to generate lithotype-dependent empirical predictive relationships between mechanical properties and log-derived petrophysical attributes/properties; (v) the estimation of uncertainties associated with predictive function parameters; (vi) the application and validation of mechanical properties predictive tools to well-documented case studies (e.g. sand strength for perforation stability, rock compressibility for reservoir simulation) to test overall performance and quantify uncertainty in predictions. This paper presents the results of various rock strength, rock compressibility and rock fracability case studies conducted in wells of different stratigraphic age and depositional environment. Overall, GML (i

  20. A rock-/ice mechanical model for the destabilisation of permafrost rocks

    NASA Astrophysics Data System (ADS)

    Krautblatter, Michael; Funk, Daniel

    2010-05-01

    The destabilisation of permafrost rocks is commonly attributed to changes in ice-mechanical properties (Davies et al. 2001). The effect of low temperatures on intact rock strength and its mechanical relevance for shear strength and brittle fracture propagation has not been considered yet. But this effect is significant since compressive and tensile strength are reduced by up to 50% and more when rock thaws (Mellor, 1973). Here we show, that the reduction of the shear resistance of rock-rock contacts in joints plays a key role for the onset of larger instabilities in thawing permafrost rocks. Based on a Mohr-Coulomb assumption, we defined a failure criterion of an ice-filled rock cleft, with cohesive rock bridges, contact of rough fracture surfaces, ductile creep of ice and with a representation of rock-ice "failure" mechanisms along the surface and inside the ice body. The synoptic models are based on the principle of superposition, i.e. that shear stress "absorbed" by one component reduces the amount of shear stress applied to the other components. Failure along existing sliding planes can be explained by the impact of temperature on shear stress uptake by creep deformation of ice, the propensity of failure along rock-ice fractures and reduced total friction along rough rock-rock contacts. This model may account for the rapid response of rockslides to warming (reaction time). In the long term, brittle fracture propagation is initialised. Warming reduces the shear stress uptake by total friction and decreases the critical fracture toughness along rock bridges. The latter model accounts for slow subcritical destabilisation of whole rock slopes over decades to millennia, subsequent to the warming impulse (relaxation time). To test the importance of reduced friction, we conducted shearing tests on homogeneous fine-grained limestone specimen taken from a permafrost site (Zugspitze, Germany). In a temperature-controlled shearing box, we repeatedly tested mechanical

  1. GENERIC THERMO-MECHANICAL MODEL FOR JOINTED ROCK MASSES

    SciTech Connect

    Vorobiev, O

    2007-03-08

    A new nonlinear thermo-mechanical model for heavily jointed rock masses is presented. The model uses correlation functions between the porosity and the basic rock properties such as elastic moduli, tensile and compressive strength. The model assumes that the media is isotropic and is characterized by two variable parameters: insipient porosity and in-situ-to-intact modulus ratio.

  2. Analyzing failure modes of rock mass based on statistical mechanics of rock mass

    NASA Astrophysics Data System (ADS)

    Bao, H.; Wu, F.

    2015-12-01

    Joints influence mechanical properties of rock mass. Based on the strength criterion of statistical mechanics of rock mass, we analyzed the four different failure modes of rock mass with a group of joints by combining with Mohr-Coulomb strength criterion. We also deduced an expression of the critical confining pressure for explaining the transformation from structure control to stress control of rock mass strength. On this basis, rock mass with a group of joints were divided into four classes according to the relations between rock mass and joints parameters. Then, the possible failure modes and their corresponding conditions were discussed. At last, the strength characteristics of diorite with a group of joints were analyzed. The results showed that the diorite belonged to class rock mass and performed significant anisotropy in compressive strength. At the condition of vertical pressure, the rock start failing after joints at the critical confining pressure of 9.12MPa. However, with the confining pressure increasing, the anisotropy of strength became weak, and the strength of diorite would convert from structure control to stress control under some particular loading directions.

  3. Rock Mechanics Models and Measurements Challenges from Industry. Proceedings

    SciTech Connect

    Laubach, S.E.; Nelson, P.P.

    1994-01-01

    Increased mutual dependence of the economies of Canada, the United States and Mexico has now been recognized formally by agreements between the respective national governments. Noting the basic economic role of rock mechanics in the resource recovery and construction industries, it is appropriate that the First North American Rock Mechanics Symposium should confirm mutual interest in rock mechanics research and engineering practice in the neighboring countries. Different government and industrial emphases in the NAFTA countries lead to complementary strengths in their research and engineering programs. The First NARM Symposium is the first opportunity to explore thoroughly, within the scope of a single meeting, rock mechanics research in progress and engineering achievements in the three countries. Individual papers abstracted separately.

  4. Mitochondrial fragmentation in excitotoxicity requires ROCK activation.

    PubMed

    Martorell-Riera, Alejandro; Segarra-Mondejar, Marc; Reina, Manuel; Martínez-Estrada, Ofelia M; Soriano, Francesc X

    2015-01-01

    Mitochondria morphology constantly changes through fission and fusion processes that regulate mitochondrial function, and it therefore plays a prominent role in cellular homeostasis. Cell death progression is associated with mitochondrial fission. Fission is mediated by the mainly cytoplasmic Drp1, which is activated by different post-translational modifications and recruited to mitochondria to perform its function. Our research and other studies have shown that in the early moments of excitotoxic insult Drp1 must be nitrosylated to mediate mitochondrial fragmentation in neurons. Nonetheless, mitochondrial fission is a multistep process in which filamentous actin assembly/disassembly and myosin-mediated mitochondrial constriction play prominent roles. Here we establish that in addition to nitric oxide production, excitotoxicity-induced mitochondrial fragmentation also requires activation of the actomyosin regulator ROCK. Although ROCK1 has been shown to phosphorylate and activate Drp1, experiments using phosphor-mutant forms of Drp1 in primary cortical neurons indicate that in excitotoxic conditions, ROCK does not act directly on Drp1 to mediate fission, but may act on the actomyosin complex. Thus, these data indicate that a wider range of signaling pathways than those that target Drp1 are amenable to be inhibited to prevent mitochondrial fragmentation as therapeutic option. PMID:25789413

  5. Mitochondrial fragmentation in excitotoxicity requires ROCK activation.

    PubMed

    Martorell-Riera, Alejandro; Segarra-Mondejar, Marc; Reina, Manuel; Martínez-Estrada, Ofelia M; Soriano, Francesc X

    2015-01-01

    Mitochondria morphology constantly changes through fission and fusion processes that regulate mitochondrial function, and it therefore plays a prominent role in cellular homeostasis. Cell death progression is associated with mitochondrial fission. Fission is mediated by the mainly cytoplasmic Drp1, which is activated by different post-translational modifications and recruited to mitochondria to perform its function. Our research and other studies have shown that in the early moments of excitotoxic insult Drp1 must be nitrosylated to mediate mitochondrial fragmentation in neurons. Nonetheless, mitochondrial fission is a multistep process in which filamentous actin assembly/disassembly and myosin-mediated mitochondrial constriction play prominent roles. Here we establish that in addition to nitric oxide production, excitotoxicity-induced mitochondrial fragmentation also requires activation of the actomyosin regulator ROCK. Although ROCK1 has been shown to phosphorylate and activate Drp1, experiments using phosphor-mutant forms of Drp1 in primary cortical neurons indicate that in excitotoxic conditions, ROCK does not act directly on Drp1 to mediate fission, but may act on the actomyosin complex. Thus, these data indicate that a wider range of signaling pathways than those that target Drp1 are amenable to be inhibited to prevent mitochondrial fragmentation as therapeutic option.

  6. Mechanical Properties of Shock-Damaged Rocks

    NASA Technical Reports Server (NTRS)

    He, Hongliang; Ahrens, T. J.

    1994-01-01

    Stress-strain tests were performed both on shock-damaged gabbro and limestone. The effective Young's modulus decreases with increasing initial damage parameter value, and an apparent work-softening process occurs prior to failure. To further characterize shock-induced microcracks, the longitudinal elastic wave velocity behavior of shock-damaged gabbro in the direction of compression up to failure was measured using an acoustic transmission technique under uniaxial loading. A dramatic increase in velocity was observed for the static compressive stress range of 0-50 MPa. Above that stress range, the velocity behavior of lightly damaged (D(sub 0) less than 0.1) gabbro is almost equal to unshocked gabbro. The failure strength of heavily-damaged (D(sub 0) greater than 0.1) gabbro is approx. 100-150 MPa, much lower than that of lightly damaged and unshocked gabbros (approx. 230-260 MPa). Following Nur's theory, the crack shape distribution was analyzed. The shock-induced cracks in gabbro appear to be largely thin penny-shaped cracks with c/a values below 5 x 10(exp -4). Moreover, the applicability of Ashby and Sammis's theory relating failure strength and damage parameter of shock-damaged rocks was examined and was found to yield a good estimate of the relation of shock-induced deficit in elastic modulus with the deficit in compressive strength.

  7. Reconstruction of Sedimentary Rock Based on MechanicalProperties

    SciTech Connect

    Jin, Guodong; Patzek, Tad W.; Silin, Dmitry B.

    2004-05-04

    We describe a general, physics-based approach to numericalreconstruction of the geometrical structure and mechanical properties ofnatural sedimentary rock in 3D. Our procedure consists of three mainsteps: sedimentation, compaction, and diagenesis, followed by theverification of rock mechanical properties. The dynamic geologicprocesses of grain sedimentation and compaction are simulated by solvinga dimensionless form of Newton's equations of motion for an ensemble ofgrains. The diagenetic rock transformation is modeled using a cementationalgorithm, which accounts for the effect of rock grain size on therelative rate of cement overgrowth. Our emphasis is on unconsolidatedsand and sandstone. The main input parameters are the grain sizedistribution, the final rock porosity, the type and amount of cement andclay minerals, and grain mechanical properties: the inter-grain frictioncoefficient, the cement strength, and the grain stiffness moduli. We usea simulated 2D Fontainebleau sandstone to obtain the grain mechanicalproperties. This Fontainebleau sandstone is also used to study theinitiation, growth, and coalescence of micro-cracks under increasingvertical stress. The box fractal dimension of the micro-crackdistribution, and its variation with the applied stress areestimated.

  8. A Preliminary Study of 3D Printing on Rock Mechanics

    NASA Astrophysics Data System (ADS)

    Jiang, Chao; Zhao, Gao-Feng

    2015-05-01

    3D printing is an innovative manufacturing technology that enables the printing of objects through the accumulation of successive layers. This study explores the potential application of this 3D printing technology for rock mechanics. Polylactic acid (PLA) was used as the printing material, and the specimens were constructed with a "3D Touch" printer that employs fused deposition modelling (FDM) technology. Unconfined compressive strength (UCS) tests and direct tensile strength (DTS) tests were performed to determine the Young's modulus ( E) and Poisson's ratio ( υ) for these specimens. The experimental results revealed that the PLA specimens exhibited elastic to brittle behaviour in the DTS tests and exhibited elastic to plastic behaviour in the UCS tests. The influence of structural changes in the mechanical response of the printed specimen was investigated; the results indicated that the mechanical response is highly influenced by the input structures, e.g., granular structure, and lattice structure. Unfortunately, our study has demonstrated that the FDM 3D printing with PLA is unsuitable for the direct simulation of rock. However, the ability for 3D printing on manufactured rock remains appealing for researchers of rock mechanics. Additional studies should focus on the development of an appropriate substitution for the printing material (brittle and stiff) and modification of the printing technology (to print 3D grains with arbitrary shapes).

  9. Mechanical and hydraulic properties of rocks related to induced seismicity

    USGS Publications Warehouse

    Witherspoon, P.A.; Gale, J.E.

    1977-01-01

    Witherspoon, P.A. and Gale, J.E., 1977. Mechanical and hydraulic properties of rocks related to induced seismicity. Eng. Geol., 11(1): 23-55. The mechanical and hydraulic properties of fractured rocks are considered with regard to the role they play in induced seismicity. In many cases, the mechanical properties of fractures determine the stability of a rock mass. The problems of sampling and testing these rock discontinuities and interpreting their non-linear behavior are reviewed. Stick slip has been proposed as the failure mechanism in earthquake events. Because of the complex interactions that are inherent in the mechanical behavior of fractured rocks, there seems to be no simple way to combine the deformation characteristics of several sets of fractures when there are significant perturbations of existing conditions. Thus, the more important fractures must be treated as individual components in the rock mass. In considering the hydraulic properties, it has been customary to treat a fracture as a parallel-plate conduit and a number of mathematical models of fracture systems have adopted this approach. Non-steady flow in fractured systems has usually been based on a two-porosity model, which assumes the primary (intergranular) porosity contributes only to storage and the secondary (fracture) porosity contributes only to the overall conductivity. Using such a model, it has been found that the time required to achieve quasi-steady state flow in a fractured reservoir is one or two orders of magnitude greater than it is in a homogeneous system. In essentially all of this work, the assumption has generally been made that the fractures are rigid. However, it is clear from a review of the mechanical and hydraulic properties that not only are fractures easily deformed but they constitute the main flow paths in many rock masses. This means that one must consider the interaction of mechanical and hydraulic effects. A considerable amount of laboratory and field data is now

  10. Increased anticancer activity of the thymidylate synthase inhibitor BGC9331 combined with the topoisomerase I inhibitor SN-38 in human colorectal and breast cancer cells: induction of apoptosis and ROCK cleavage through caspase-3-dependent and -independent mechanisms.

    PubMed

    Coudray, Anne-Marie; Louvet, Christophe; Kornprobst, Michel; Raymond, Eric; André, Thierry; Tournigand, Christophe; Faivre, Sandrine; De Gramont, Aimery; Larsen, Annette K; Gespach, Christian

    2005-08-01

    The folate analogue BGC9331 is a new thymidylate synthase (TS) inhibitor showing a broad spectrum of cyto-toxic activity against several human solid tumors, including colorectal cancer. In this study, we investigated the anticancer activity of BGC9331 either alone or combined with 5-fluorouracil (5-FU), MTA (multi-target antifolate), oxali-platin and SN-38, the active metabolite of the topoisomerase I inhibitor CPT-11. The antiproliferative activity of each drug and BGC9331-based combinations was investigated in the HT-29 human colorectal cancer cell line and its HT-29/5-FU counterparts selected for resistance to 5-FU. BGC9331 combined with MTA or SN-38 induced synergistic responses in HT-29 cells. Treatment of HT-29 cells with either BGC9331 or SN-38 increased caspase-3 activity and the percentage of apoptotic cells from 3 to 13%. Both drugs also augmented the proteolytic cleavage of the Rho-kinase ROCK-1 that was attenuated by the caspase-3 pathway inhibitor z-DEVD-fmk. BGC9331 combined with SN-38 further increased the percentage of apoptotic cells to 25%, and inhibited cell cycle progression and cell proliferation by 65%. This was accompanied by proteolytic activation of ROCK-1, through both caspase-3-dependent and -independent mechanisms, as shown in caspase-3-deficient MCF-7 breast cancer cells. These encouraging results warrant further preclinical investigations and clinical trials on the use of BGC9331 combined with SN-38/CPT-11 in treatment of patients with advanced colorectal or gastric cancers.

  11. A mechanism for high wall-rock velocities in rockbursts

    USGS Publications Warehouse

    McGarr, A.

    1997-01-01

    Considerable evidence has been reported for wall-rock velocities during rockbursts in deep gold mines that are substantially greater than ground velocities associated with the primary seismic events. Whereas varied evidence suggests that slip across a fault at the source of an event generates nearby particle velocities of, at most, several m/s, numerous observations, in nearby damaged tunnels, for instance, imply wall-rock velocities of the order of 10 m/s and greater. The common observation of slab buckling or breakouts in the sidewalls of damaged excavations suggests that slab flexure may be the mechanism for causing high rock ejection velocities. Following its formation, a sidewall slab buckles, causing the flexure to increase until the stress generated by flexure reaches the limit 5 that can be supported by the sidewall rock. I assume here that S is the uniaxial compressive strength. Once the flexural stress exceeds S, presumably due to the additional load imposed by a nearby seismic event, the slab fractures and unflexes violently. The peak wall-rock velocity v thereby generated is given by v=(3 + 1-??2/2)1 2 S/?????E for rock of density ??, Young's modulus E, and Poisson's ratio ??. Typical values of these rock properties for the deep gold mines of South Africa yield v= 26 m/s and for especially strong quartzites encountered in these same mines, v> 50m/s. Even though this slab buckling process leads to remarkably high ejection velocities and violent damage in excavations, the energy released during this failure is only a tiny fraction of that released in the primary seismic event, typically of magnitude 2 or greater.

  12. Lithophysal Rock Mass Mechanical Properties of the Repository Host Horizon

    SciTech Connect

    D. Rigby

    2004-11-10

    The purpose of this calculation is to develop estimates of key mechanical properties for the lithophysal rock masses of the Topopah Spring Tuff (Tpt) within the repository host horizon, including their uncertainties and spatial variability. The mechanical properties to be characterized include an elastic parameter, Young's modulus, and a strength parameter, uniaxial compressive strength. Since lithophysal porosity is used as a surrogate property to develop the distributions of the mechanical properties, an estimate of the distribution of lithophysal porosity is also developed. The resulting characterizations of rock parameters are important for supporting the subsurface design, developing the preclosure safety analysis, and assessing the postclosure performance of the repository (e.g., drift degradation and modeling of rockfall impacts on engineered barrier system components).

  13. Two functional polymorphisms of ROCK2 enhance arterial stiffening through inhibiting its activity and expression.

    PubMed

    Liao, Yi-Chu; Liu, Ping-Yen; Lin, Hsiu-Fen; Lin, Wen-Yi; Liao, James K; Juo, Suh-Hang H

    2015-02-01

    Derangement of Rho-associated kinases (ROCKs) has been related to coronary artery disease and stroke. ROCK2, rather than ROCK1, plays a predominant role in vascular contractility. The present study aims to test (1) the associations between ROCK2 single nucleotide polymorphisms (SNPs) and arterial stiffness, and (2) the molecular mechanism accounting for their effects. Stiffness parameters including beta (β), elasticity modulus (Ep) and pulse wave velocity (PWV) were obtained by carotid ultrasonography. Seven tagging SNPs of ROCK2 were initially genotyped in 856 subjects and significant SNPs were replicated in another group of 527 subjects. Two SNPs in complete linkage disequilibrium were found to be significantly associated with arterial stiffness. The major alleles of rs978906 (A allele) and rs9808232 (C allele) were associated with stiffer arteries. SNP rs978906 was predicted to influence microRNA(miR)-1183 binding to ROCK2, while rs9808232 causes amino acid substitution. To determine their functional impact, plasmid constructs carrying different alleles of the significant SNPs were created. Compared to rs978906G-allele constructs, cells transfected with rs978906A-allele constructs had higher baseline luciferase activities and were less responsive to miR-1183 changes. Oxidized-low density lipoprotein (Ox-LDL) suppressed miR-1183 levels and increased ROCK2 protein amounts. For rs9808232, cells transfected with C-allele constructs had significantly higher ROCK activities than those with A-allele constructs. Leukocyte ROCK activities were further measured in 52 healthy subjects. The average ROCK activity was highest in human subjects with CC genotype at rs9808232, followed by those with AC and lowest in AA. Taken together, the present study showed that two functional SNPs of ROCK2 increase susceptibility of arterial stiffness in the Chinese population. Non-synonymous SNP rs9808232 influences ROCK2 activity, while 3' UTR SNP rs978906 affects the ROCK2 protein

  14. Two functional polymorphisms of ROCK2 enhance arterial stiffening through inhibiting its activity and expression

    PubMed Central

    Liao, Yi-Chu; Liu, Ping-Yen; Lin, Hsiu-Fen; Lin, Wen-Yi; Liao, James K.; Juo, Suh-Hang H.

    2016-01-01

    Derangement of Rho-associated kinases (ROCKs) has been related to coronary artery disease and stroke. ROCK2, rather than ROCK1, plays a predominant role in vascular contractility. The present study aims to test (1) the associations between ROCK2 single nucleotide polymorphisms (SNPs) and arterial stiffness, and (2) the molecular mechanism accounting for their effects. Stiffness parameters including beta (β), elasticity modulus (Ep) and pulse wave velocity (PWV) were obtained by carotid ultrasonography. Seven tagging SNPs of ROCK2 were initially genotyped in 856 subjects and significant SNPs were replicated in another group of 527 subjects. Two SNPs in complete linkage disequilibrium were found to be significantly associated with arterial stiffness. The major alleles of rs978906 (A allele) and rs9808232 (C allele) were associated with stiffer arteries. SNP rs978906 was predicted to influence microRNA(miR)-1183 binding to ROCK2, while rs9808232 causes amino acid substitution. To determine their functional impact, plasmid constructs carrying different alleles of the significant SNPs were created. Compared to rs978906G-allele constructs, cells transfected with rs978906A-allele constructs had higher baseline luciferase activities and were less responsive to miR-1183 changes. Oxidized-low density lipoprotein (Ox-LDL) suppressed miR-1183 levels and increased ROCK2 protein amounts. For rs9808232, cells transfected with C-allele constructs had significantly higher ROCK activities than those with A-allele constructs. Leukocyte ROCK activities were further measured in 52 healthy subjects. The average ROCK activity was highest in human subjects with CC genotype at rs9808232, followed by those with AC and lowest in AA. Taken together, the present study showed that two functional SNPs of ROCK2 increase susceptibility of arterial stiffness in the Chinese population. Non-synonymous SNP rs9808232 influences ROCK2 activity, while 3' UTR SNP rs978906 affects the ROCK2 protein

  15. Some observations on the mechanism of aircraft wing rock

    NASA Technical Reports Server (NTRS)

    Hwang, C.; Pi, W. S.

    1979-01-01

    A scale model of the Northrop F-5A was tested in NASA Ames Research Center Eleven-Foot Transonic Tunnel to simulate the wing rock oscillations in a transonic maneuver. For this purpose, a flexible model support device was designed and fabricated, which allowed the model to oscillate in roll at the scaled wing rock frequency. Two tunnel entries were performed to acquire the pressure (steady state and fluctuating) and response data when the model was held fixed and when it was excited by flow to oscillate in roll. Based on these data, a limit cycle mechanism was identified, which supplied energy to the aircraft model and caused the Dutch roll type oscillations, commonly called wing rock. The major origin of the fluctuating pressures that contributed to the limit cycle was traced to the wing surface leading edge stall and the subsequent lift recovery. For typical wing rock oscillations, the energy balance between the pressure work input and the energy consumed by the model's aerodynamic and mechanical damping was formulated and numerical data presented.

  16. Some observations on the mechanism of aircraft wing rock

    NASA Technical Reports Server (NTRS)

    Hwang, C.; Pi, W. S.

    1978-01-01

    A pressure scale model of Northrop F-5A was tested in NASA Ames Research Center Eleven-Foot Transonic Tunnel to simulate the wing rock oscillations in a transonic maneuver. For this purpose, a flexible model support device was designed and fabricated which allowed the model to oscillate in roll at the scaled wing rock frequency. Two tunnel entries were performed to acquire the pressure (steady state and fluctuating) and response data when the model was held fixed and when it was excited by flow to oscillate in roll. Based on these data, a limit cycle mechanism was identified which supplied energy to the aircraft model and caused the Dutch roll type oscillations, commonly called wing rock. The major origin of the fluctuating pressures which contributed to the limit cycle was traced to the wing surface leading edge stall and the subsequent lift recovery. For typical wing rock oscillations, the energy balance between the pressure work input and the energy consumed by the model aerodynamic and mechanical damping was formulated and numerical data presented.

  17. Effect of Rapid Thermal Cooling on Mechanical Rock Properties

    NASA Astrophysics Data System (ADS)

    Kim, Kwangmin; Kemeny, John; Nickerson, Mark

    2014-11-01

    Laboratory tests have been conducted to investigate the effects of rapid thermal cooling on various rock specimens including igneous, sedimentary, and metamorphic rocks. At first, various types of thermal loading were conducted: heating up to 100, 200, and 300 °C, followed by rapid cooling with a fan. In addition, multiple cyclic thermal cooling (10, 15 and 20 cycles) with a maximum temperature of only 100 °C was conducted. Experiments included edge notched disc (END) tests to determine the Mode I fracture toughness, Brazilian disc tests to determine tensile strength, seismic tests to determine P-wave velocity, and porosity tests leading to meaningful results. Even though only small changes of temperature (rapid cooling from 100 °C to room temperature) were applied, the results showed that crack growth occurred in some rock types (granite, diabase with ore veins, and KVS) while crack healing occurred in other rock types (diabase without ore veins, quartzite, and skarn). To better understand the results, 3D transient thermo-mechanical analysis was conducted using the ANSYS program. The results indicated that there was a thin region near the outside of the specimen where large tensile stresses occur and microcracking would be expected, and that there was a large area in the middle of the specimen where lower magnitude compressive stresses occur and crack closure would be expected. It was found that the more heterogeneous and more coarse-grained rock types are more likely to exhibit crack growth, while less heterogeneous and more fine-grained rocks are more likely to exhibit crack healing.

  18. Neutron Activation Analysis for the Demonstration of Amphibolite Rock-Weathering Activity of a Yeast

    PubMed Central

    Rades-Rohkohl, E.; Hirsch, P.; Fränzle, O.

    1979-01-01

    Neutron activation analysis was employed in a survey of weathering abilities of rock surface microorganisms. A yeast isolated from an amphibolite of a megalithic grave was found actively to concentrate, in media and in or on cells, iron and other elements when grown in the presence of ground rock. This was demonstrated by comparing a spectrum of neutron-activated amphibolite powder (particle size, 50 to 100 μm) with the spectra of neutron-activated, lyophilized yeast cells which had grown with or without amphibolite powder added to different media. The most active yeast (IFAM 1171) did not only solubilize Fe from the rock powder, but significant amounts of Co, Eu, Yb, Ca, Ba, Sc, Lu, Cr, Th, and U were also mobilized. The latter two elements occurred as natural radioactive isotopes in this amphibolite. When the yeast cells were grown with neutron-activated amphibolite, the cells contained the same elements. Furthermore, the growth medium contained Fe, Co, and Eu which had been solubilized from the amphibolite. This indicates the presence, in this yeast strain, of active rockweathering abilities as well as of uptake mechanisms for solubilized rock components. PMID:16345472

  19. The variation of the mechanical properties of rock on spatial scales from the laboratory to outcrop

    NASA Astrophysics Data System (ADS)

    Gage, J.; Wang, H. F.; Fratta, D.; Maclaughlin, M.; Turner, A. L.; GEOX^TM

    2011-12-01

    We have installed a dense array of Fiber Bragg Grating (FBG) strain and temperature sensors on the 4100'-level (1250 m) at the site of the former Homestake gold mine in Lead, SD. The sensor installation site is composed of the Precambrian Poorman formation that contains deformed and metamorphosed Precambrian sediments that is anisotropic including a well-developed foliation, quartz veins, and several joint sets. We have installed nine Micron Optics Inc. OS3600 tube gages. Four of these gages are mounted on the surface of the rock mass and attached to rock bolts that extend 2 m into the rock mass. The other five OS3600 sensors are embedded in drill holes into the rock mass. Additionally, we have developed a new method for measuring in situ strain and temperature in intact rock masses. Fiber optically instrumented rock strain and temperature strips (FROSTS) are 2 m-long strips of 304 stainless steel specially designed to measure temperature and both shortening and elongation in an intact rock mass. FROSTS have FBG strain and temperature sensors mounted on them at 30 cm interval and are grouted into a drill hole in a rock mass. In May 2011, we performed an active loading experiment that consisted of using two hydraulic rams to apply over 200 kN of force to the rock mass. Elastic strain was measured with the fiber optic sensor array. A one-dimensional Boussinesq solution calculates a Young's Modulus of 6.25 GPa for the rock mass. The laboratory-determined values for Young's Modulus in the Poorman formation vary between 49.6 and 94.5 GPa. The difference between the laboratory and field values can be attributed to the closing of fractures and microcracks in the rock mass making the rock mass more compliant than the smaller specimens used for the laboratory experiments. The results of the active loading experiment have implications for the up-scaling of rock mechanical properties between the laboratory and field scales.

  20. Integrating rock mechanics issues with repository design through design process principles and methodology

    SciTech Connect

    Bieniawski, Z.T.

    1996-04-01

    A good designer needs not only knowledge for designing (technical know-how that is used to generate alternative design solutions) but also must have knowledge about designing (appropriate principles and systematic methodology to follow). Concepts such as {open_quotes}design for manufacture{close_quotes} or {open_quotes}concurrent engineering{close_quotes} are widely used in the industry. In the field of rock engineering, only limited attention has been paid to the design process because design of structures in rock masses presents unique challenges to the designers as a result of the uncertainties inherent in characterization of geologic media. However, a stage has now been reached where we are be able to sufficiently characterize rock masses for engineering purposes and identify the rock mechanics issues involved but are still lacking engineering design principles and methodology to maximize our design performance. This paper discusses the principles and methodology of the engineering design process directed to integrating site characterization activities with design, construction and performance of an underground repository. Using the latest information from the Yucca Mountain Project on geology, rock mechanics and starter tunnel design, the current lack of integration is pointed out and it is shown how rock mechanics issues can be effectively interwoven with repository design through a systematic design process methodology leading to improved repository performance. In essence, the design process is seen as the use of design principles within an integrating design methodology, leading to innovative problem solving. In particular, a new concept of {open_quotes}Design for Constructibility and Performance{close_quotes} is introduced. This is discussed with respect to ten rock mechanics issues identified for repository design and performance.

  1. The Usability of Noise Level from Rock Cutting for the Prediction of Physico-Mechanical Properties of Rocks

    NASA Astrophysics Data System (ADS)

    Delibalta, M. S.; Kahraman, S.; Comakli, R.

    2015-11-01

    Because the indirect tests are easier and cheaper than the direct tests, the prediction of rock properties from the indirect testing methods is important especially for the preliminary investigations. In this study, the predictability of the physico-mechanical rock properties from the noise level measured during cutting rock with diamond saw was investigated. Noise measurement test, uniaxial compressive strength (UCS) test, Brazilian tensile strength (BTS) test, point load strength (Is) test, density test, and porosity test were carried out on 54 different rock types in the laboratory. The results were statistically analyzed to derive estimation equations. Strong correlations between the noise level and the mechanical rock properties were found. The relations follow power functions. Increasing rock strength increases the noise level. Density and porosity also correlated strongly with the noise level. The relations follow linear functions. Increasing density increases the noise level while increasing porosity decreases the noise level. The developed equations are valid for the rocks with a compressive strength below 150 MPa. Concluding remark is that the physico-mechanical rock properties can reliably be estimated from the noise level measured during cutting the rock with diamond saw.

  2. The Rho GTPase effector ROCK regulates cyclin A, cyclin D1, and p27Kip1 levels by distinct mechanisms.

    PubMed

    Croft, Daniel R; Olson, Michael F

    2006-06-01

    The members of the Rho GTPase family are well known for their regulation of actin cytoskeletal structures. In addition, they influence progression through the cell cycle. The RhoA and RhoC proteins regulate numerous effector proteins, with a central and vital signaling role mediated by the ROCK I and ROCK II serine/threonine kinases. The requirement for ROCK function in the proliferation of numerous cell types has been revealed by studies utilizing ROCK-selective inhibitors such as Y-27632. However, the mechanisms by which ROCK signaling promotes cell cycle progression have not been thoroughly characterized. Using a conditionally activated ROCK-estrogen receptor fusion protein, we found that ROCK activation is sufficient to stimulate G1/S cell cycle progression in NIH 3T3 mouse fibroblasts. Further analysis revealed that ROCK acts via independent pathways to alter the levels of cell cycle regulatory proteins: cyclin D1 and p21(Cip1) elevation via Ras and the mitogen-activated protein kinase pathway, increased cyclin A via LIM kinase 2, and reduction of p27(Kip1) protein levels. Therefore, the influence of ROCK on cell cycle regulatory proteins occurs by multiple independent mechanisms.

  3. MECHANICAL DEGRADATION OF EMPLACEMENT DRIFTS AT YUCCA MOUNTAIN - A CASE STUDY IN ROCK MECHANICS, PART 1: NONLITHOPHYSAL ROCK, PART 2: LITHOPHYSAL ROCK

    SciTech Connect

    M. Lin, D. Kicker, B. Damjanac, M. Board, and M. Karakouzian

    2006-02-27

    This paper outlines rock mechanics investigations associated with mechanical degradation of planned emplacement drifts at Yucca Mountain, which is the designated site for a US high-level nuclear waste repository. The factors leading to drift degradation include stresses from the overburden, stresses induced by the heat released from the emplaced waste, stresses due to seismically related ground motions, and time-dependent strength degradation. The welded tuff emplacement horizon consists of two groups of rock with distinct engineering properties: nonlithophysal units and lithophysal units, based on the relative proportion of lithophysal cavities. Part I of the paper concentrates on the generally hard, strong, and fractured nonlithophysal rock. The degradation behavior of the tunnels in the nonlithophysal rock is controlled by the occurrence of keyblocks. A statistically equivalent fracture model was generated based on extensive underground fracture mapping data from the Exploratory Studies Facility at Yucca Mountain. Three-dimensional distinct block analyses, generated with the fracture patterns randomly selected from the fracture model, were developed with the consideration of in situ, thermal, seismic loads. In this study, field data, laboratory data, and numerical analyses are well integrated to provide a solution for the unique problem of modeling drift degradation throughout the regulatory period for repository performance.

  4. ACOUSTICAL IMAGING AND MECHANICAL PROPERTIES OF SOFT ROCK AND MARINE SEDIMENTS

    SciTech Connect

    Thurman E. Scott, Jr.; Younane Abousleiman

    2004-04-01

    The research during this project has concentrated on developing a correlation between rock deformation mechanisms and their acoustic velocity signature. This has included investigating: (1) the acoustic signature of drained and undrained unconsolidated sands, (2) the acoustic emission signature of deforming high porosity rocks (in comparison to their low porosity high strength counterparts), (3) the effects of deformation on anisotropic elastic and poroelastic moduli, and (4) the acoustic tomographic imaging of damage development in rocks. Each of these four areas involve triaxial experimental testing of weak porous rocks or unconsolidated sand and involves measuring acoustic properties. The research is directed at determining the seismic velocity signature of damaged rocks so that 3-D or 4-D seismic imaging can be utilized to image rock damage. These four areas of study are described in the report: (1) Triaxial compression experiments have been conducted on unconsolidated Oil Creek sand at high confining pressures. (2) Initial experiments on measuring the acoustic emission activity from deforming high porosity Danian chalk were accomplished and these indicate that the AE activity was of a very low amplitude. (3) A series of triaxial compression experiments were conducted to investigate the effects of induced stress on the anisotropy developed in dynamic elastic and poroelastic parameters in rocks. (4) Tomographic acoustic imaging was utilized to image the internal damage in a deforming porous limestone sample. Results indicate that the deformation damage in rocks induced during laboratory experimentation can be imaged tomographically in the laboratory. By extension the results also indicate that 4-D seismic imaging of a reservoir may become a powerful tool for imaging reservoir deformation (including imaging compaction and subsidence) and for imaging zones where drilling operation may encounter hazardous shallow water flows.

  5. Compaction bands in porous rocks: localization analysis using breakage mechanics

    NASA Astrophysics Data System (ADS)

    Das, Arghya; Nguyen, Giang; Einav, Itai

    2010-05-01

    It has been observed in fields and laboratory studies that compaction bands are formed within porous rocks and crushable granular materials (Mollema and Antonellini, 1996; Wong et al., 2001). These localization zones are oriented at high angles to the compressive maximum principal stress direction. Grain crushing and pore collapse are the integral parts of the compaction band formation; the lower porosity and increased tortuosity within such bands tend to reduce their permeability compared to the outer rock mass. Compaction bands may thereafter act as flow barriers, which can hamper the extraction or injection of fluid into the rocks. The study of compaction bands is therefore not only interesting from a geological viewpoint but has great economic importance to the extraction of oil or natural gas in the industry. In this paper, we study the formation of pure compaction bands (i.e. purely perpendicular to the principal stress direction) or shear-enhanced compaction bands (i.e. with angles close to the perpendicular) in high-porosity rocks using both numerical and analytical methods. A model based on the breakage mechanics theory (Einav, 2007a, b) is employed for the present analysis. The main aspect of this theory is that it enables to take into account the effect that changes in grain size distribution has on the constitutive stress-strain behaviour of granular materials at the microscopic level due to grain crushing. This microscopic phenomenon of grain crushing is explicitly linked with a macroscopic internal variable, called Breakage, so that the evolving grain size distribution can be continuously monitored at macro scale during the process of deformation. Through the inclusion of an appropriate parameter the model is also able to capture the effects of pore collapse on the macroscopic response. Its possession of few physically identifiable parameters is another important feature which minimises the effort of their recalibration, since those become less

  6. Probing Mechanical Properties of Rock with InSAR

    NASA Astrophysics Data System (ADS)

    Jónsson, S.

    2012-04-01

    Interferometric Synthetic Aperture Radar (InSAR) observations from satellites have revolutionized our crustal deformation measurement capabilities with its high spatial resolution, global coverage, and low cost. The high spatial resolution (typically 5-20 m) allows us to map many small-scale surface deformation phenomena in great detail. These include surface faulting, fissuring, fault creep, and other strain localization phenomena. Another advantage of the small-scale deformation mapping is that it can provide information about mechanical properties of near-surface rocks. Several studies have already been published on using InSAR to probe material properties of rock. Strain localizations at fault zones have been observed in co-seismic deformation fields near to large earthquakes and interpreted as expressions of weak fault zone materials that are a factor of two more compliant than the surrounding unbroken rock [Fialko et al., 2002]. Peltzer et al. [1999] argued that asymmetries in coseismic deformation patterns observed by InSAR showed evidence for non-linear elasticity, i.e. that the elastic moduli of shallow crustal material are different for compression and extension, due to small-scale cracks in the medium. This interpretation was later disputed by Funning et al. [2007], who provided an alternative explanation for observed deformation pattern based on along-strike variations in fault geometry and slip. In addition, observations and modeling of poro-elastic rebound after earthquakes have provided information about the difference in undrained and drained Poisson's ratio values of the near-surface rocks [Peltzer et al., 1996; Jónsson et al., 2003]. More recently we have used InSAR observations to put bounds on the tensional bulk strength of surface rocks. A dyke intrusion that took place in western Saudi Arabia in 2009 caused many moderate-sized earthquakes and extensive surface faulting. InSAR data of the area show that large-scale (40 km x 40 km) east

  7. Mechanisms of rock slope failure in conglomerates with variable lithification

    NASA Astrophysics Data System (ADS)

    Bundschuh, M.; Thuro, K.

    2012-04-01

    In conglomerates with variable lithification very special failure mechanisms my occur. On January 25th, 2010 at the village of Stein at the river Traun, at about 8 p.m. a 432 m3 large and 1,026 t heavy block was released from a conglomerate face obviously without warning, destroying the family home below. Only two of four inhabitants could be saved out of the debris by means of a spectacular rescue operation through the local fire brigade. After this event the question arised, if the rock fall could have been foreseen or if such spontaneous incidents are abrupt and unpredictable. In this paper the conducted studies to reconstruct the processes leading to this event will be presented. These investigations included field mapping, geodetic survey, laserscanning of the rupture face, mineralogical analysis of sinter crust thin sections, inventory of the block dimensions and reconstruction of the collapse kinematics, analysis of the weather data prior to the event and a 2D finite element calculation (Phase2, rocscience) using the geometry of the overhanging conglomerate strata. In this case, it seems like there was no clear triggering event prior to the wall collapse. Instead, it could be proved by engineering geology mapping, mineralogical analysis of the sinter crusts and numerical modelling, that the back scarp connected with a set of discontinuities started to propagate several years ago already. Also supported by early photographs of the cracks in the brick walls of the endangered house in 1993 and 2006 together with eye wittnesses, it could be shown, that the fracture propagation started tens of years beforehand and the rock topple - rock fall took place after the last rock bond bridges finally were sheared through. As a result of all field data and the numerical modeling, the causes of the event can be stated as: • caving in the rock mass of up to 9 m depth at the foot oft he wall; • the low strength values of the conglomerates; and • vertical joint sets

  8. Rocks.

    ERIC Educational Resources Information Center

    Lee, Alice

    This science unit is designed for limited- and non-English speaking students in a Chinese bilingual education program. The unit covers rock material, classification, characteristics of types of rocks, and rock cycles. It is written in Chinese and simple English. At the end of the unit there is a list of main terms in both English and Chinese, and…

  9. Numerical method to determine mechanical parameters of engineering design in rock masses.

    PubMed

    Xue, Ting-He; Xiang, Yi-Qiang; Guo, Fa-Zhong

    2004-07-01

    This paper proposes a new continuity model for engineering in rock masses and a new schematic method for reporting the engineering of rock continuity. This method can be used to evaluate the mechanics of every kind of medium; and is a new way to determine the mechanical parameters used in engineering design in rock masses. In the numerical simulation, the experimental parameters of intact rock were combined with the structural properties of field rock. The experimental results for orthogonally-jointed rock are given. The results included the curves of the stress-strain relationship of some rock masses, the curve of the relationship between the dimension Delta and the uniaxial pressure-resistant strength sc of these rock masses, and pictures of the destructive procedure of some rock masses in uniaxial or triaxial tests, etc. Application of the method to engineering design in rock masses showed the potential of its application to engineering practice.

  10. [Synthesis of anabiosis autoinducers in non-spore-forming bacteria as a mechanism regulating their activity in soil and subsoil sedimentary rocks].

    PubMed

    Muliukin, A L; Demkina, E V; Kozlova, A N; Soina, V S; El'-Registan, G I

    2001-01-01

    Non-spore-forming bacteria of the genera Arthrobacter and Micrococcus, isolated from permafrost subsoil, were found to produce greater amounts of the d1 extracellular factor than closely related collection strains isolated from soil. The effect of this factor, responsible for cell transition to anabiosis, was not species-specific. Thus, the d1 crude preparation isolated from the culture liquid of the permafrost isolate Arthrobacter globiformis 245 produced an effect on the collection strain Arthrobacter globiformis B-1112 and also on Micrococcus luteus and Bacillus cereus. The crude d1 preparation from the permafrost isolate of Arthrobacter differed from the chemical analogue of this factor, 4n-hexylresorcinol, in the level of the induced cell response, which may have resulted from different cell sensitivity to various homologs of alkylhydroxybenzenes contained in the d1 preparation. Thus, additional evidence was obtained indicating that autoregulation of bacterial growth and development is implemented at the level of intercellular interactions in microbial communities. Abundant production of the d1 anabiosis-inducing factors by bacteria isolated from permafrost subsoil is probably a result of special antistress mechanisms responsible for the survival of these bacteria under extreme conditions of natural deep cooling.

  11. Nondestructive Methods to Characterize Rock Mechanical Properties at Low-Temperature: Applications for Asteroid Capture Technologies

    NASA Astrophysics Data System (ADS)

    Savage, Kara A.

    Recent government initiatives and commercial activities have targeted asteroids for in situ material characterization, manipulation, and possible resource extraction. Most of these activities and missions have proposed significant robotic components, given the risks and costs associated with manned missions. To successfully execute these robotic activities, detailed mechanical characteristics of the target space bodies must be known prior to contact, in order to appropriately plan and direct the autonomous robotic protocols. Unfortunately, current estimates of asteroid mechanical properties are based on limited direct information, and significant uncertainty remains specifically concerning internal structures, strengths, and elastic properties of asteroids. One proposed method to elucidate this information is through in situ, nondestructive testing of asteroid material immediately after contact, but prior to any manipulation or resource extraction activities. While numerous nondestructive rock characterization techniques have been widely deployed for terrestrial applications, these methods must be adapted to account for unique properties of asteroid material and environmental conditions of space. For example, asteroid surface temperatures may range from -100°C to 30°C due to diurnal cycling, and these low temperatures are especially noteworthy due to their deleterious influence on non-destructive testing. As a result, this thesis investigates the effect of low temperature on the mechanical characteristics and nondestructive technique responses of rock material. Initially, a novel method to produce low temperature rock samples was developed. Dry ice and methanol cooling baths of specific formulations were used to decrease rock to temperatures ranging from -60°C to 0°C. At these temperatures, shale, chalk, and limestone rock samples were exposed to several nondestructive and conventional mechanical tests, including Schmidt hammer, ultrasonic pulse velocity, point

  12. Site study plan for routine laboratory rock mechanics, Deaf Smith County Site, Texas: Revision 1

    SciTech Connect

    Not Available

    1987-12-01

    This Site Study Plan for Routine Laboratory Rock Mechanics describes routine laboratory testing to be conducted on rock samples collected as part of the characterization of the Deaf Smith County site, Texas. This study plan describes the early laboratory testing. Additional testing may be required and the type and scope of testing will be dependent upon the results of the early testing. This study provides for measurements of index, hydrological, mechanical, and chemical properties with tests which are standardized and used widely in geotechnical investigations. Another Site Study Plan for Nonroutine Laboratory Rock Mechanics describes laboratory testing of samples from the site to determine mechanical, thermomechanical, and thermal properties by less widely used methods, many of which have been developed specifically for characterization of the site. Data from laboratory tests will be used for characterization of rock strata, design of shafts and underground facilities, and modeling of repository behavior in support of resolution of both preclosure and postclosure issues. A tentative testing schedule and milestone log are given. A quality assurance program will be utilized to assure that activities affecting quality are performed correctly and that appropriate documentation is maintained. 18 refs., 8 figs., 3 tabs.

  13. Towards a mechanical failure model for degrading permafrost rock slopes representing changes in rock toughness and infill

    NASA Astrophysics Data System (ADS)

    Mamot, Philipp; Krautblatter, Michael; Scandroglio, Riccardo

    2016-04-01

    The climate-induced degradation of permafrost in mountain areas can reduce the stability of rock slopes. An increasing number of rockfalls and rockslides originate from permafrost-affected rock faces. Discontinuity patterns and their geometrical and mechanical properties play a decisive role in controlling rock slope stability. Under thawing conditions the shear resistance of rock reduces due to lower friction along rock-rock contacts, decreasing fracture toughness of rock-ice contacts, diminishing fracture toughness of cohesive rock bridges and altered creep or fracture of the ice itself. Compressive strength is reduced by 20 to 50 % and tensile strength decreases by 15 to 70 % when intact saturated rock thaws (KRAUTBLATTER ET AL. 2013). Elevated water pressures in fractures can lead to reduced effective normal stresses and thus to lower shear strengths of fractures. However, the impact of degrading permafrost on the mechanical properties of intact or fractured rock still remains poorly understood. In this study, we develop a new approach for modeling the influence of degrading permafrost on the stability of high mountain rock slopes. Hereby, we focus on the effect of rock- and ice-mechanical changes along striking discontinuities onto the whole rock slope. We aim at contributing to a better rock-ice mechanical process understanding of degrading permafrost rocks. For parametrisation and subsequent calibration of our model, we chose a test site (2885 m a.s.l.) close by the Zugspitze summit in Germany. It reveals i) a potential rockslide at the south face involving 10E4m³ of rock and ii) permafrost occurrence due to ice-filled caves and fractures. Here we combine kinematic, geotechnical and thermal monitoring in the field with rock-mechanical laboratory tests and a 2D numerical failure modeling. Up to date, the following results underline the potential effects of thawing rock and fracture infill on the stability of steep rock slopes in theory and praxis: i. ERT and

  14. Deep-seated slowly moving rock slides in foliated metamorphic rock masses: New findings about kinematical and hydro-mechanical processes

    NASA Astrophysics Data System (ADS)

    Zangerl, Christian; Strauhal, Thomas; Holzmann, Michael

    2013-04-01

    Deep-seated slowly moving rock slides are characterised by deformation along one or several shear zones where most of the measured total slope displacement localizes. Many of these rock slides move downwards at mean annual rates of some centimetres or even less and do not show any evidence for non-reversible acceleration in the past or in the future. Whereas some of these rock slides are currently inactive (dormant) or have even reached a stabilised final state others show a temporally variable deformation behaviour characterized by low base activities superimposed by acceleration phases. The trigger for these phases can be manifold and include heavy rainfall, snow melt, water level fluctuations of reservoirs, changes in the slope's equilibrium state due to antecedent slow creeping processes, variations in the material behaviour within the shear/sliding zone, erosion along the foot of the slope, etc. In order to improve the understanding of the activity behaviour and trigger factors, to increase the quality of slope stability analyses and to assess the hazard potential detailed information about the rock slide geometry and kinematics are essential. Given that subsurface investigations such as boreholes and investigation adits on large-scale rock slides are costly, most published studies are related to investigations in the surroundings of infrastructures and human settlements. Within this study new field mapping, deformation monitoring, geophysical exploration and in-situ subsurface investigation data are presented which are obtained on case studies in paragneissic rock masses of the Austroalpine Ötztal-Stubai complex (Tyrol, Austria). The new investigations contribute to develop geometrical rock slide models, to study the internal deformation characteristics of the rock slide mass and to develop kinematical deformation models. In addition, results show that all case studies are characterised by slope deformation mechanisms due to shear/slide processes along dm to

  15. Mechanical twinning as stress indicator in fault rocks

    NASA Astrophysics Data System (ADS)

    Wenk, H.

    2011-12-01

    At low stresses and elevated temperatures rocks deform by dislocation movements and diffusion. At very high stresses they undergo brittle failure. For many minerals there is an intermediate regime where mechanical twinning occurs. This has been studied extensively in calcite (Turner, Griggs and Heard, GSA Mem. 1954) and also documented for quartz (Tullis, Science, 1972). In this study we use twinning microstructures to characterize rocks that were subjected to seismic stresses, specifically pseudotachylites and samples from the San Andreas Fault Observatory at Depth. For calcite in SAFOD samples, dislocation densities derived from TEM images as well as twin densities measured by optical microscopy, indicate stresses between 50 and 200 MPa. Similar residual stress magnitudes were obtained from preserved lattice distortion determined by synchrotron X-ray microdiffraction. Also quartz shows characteristic twin microstructures. Orientation maps with SEM-EBSD reveal that quartz associated with pseudotachylite veins is profusely twinned, similar to structures observed in quartz subjected to meteorite impacts. It suggests that local dynamic seismic stresses during earthquakes are responsible for Dauphiné twinning. Thus microstructures in minerals add information to constrain macroscopic conditions during faulting.

  16. Double-Edge Sword of Sustained ROCK Activation in Prion Diseases through Neuritogenesis Defects and Prion Accumulation

    PubMed Central

    Alleaume-Butaux, Aurélie; Nicot, Simon; Pietri, Mathéa; Baudry, Anne; Dakowski, Caroline; Tixador, Philippe; Ardila-Osorio, Hector; Haeberlé, Anne-Marie; Bailly, Yannick; Peyrin, Jean-Michel; Launay, Jean-Marie; Kellermann, Odile; Schneider, Benoit

    2015-01-01

    In prion diseases, synapse dysfunction, axon retraction and loss of neuronal polarity precede neuronal death. The mechanisms driving such polarization defects, however, remain unclear. Here, we examined the contribution of RhoA-associated coiled-coil containing kinases (ROCK), key players in neuritogenesis, to prion diseases. We found that overactivation of ROCK signaling occurred in neuronal stem cells infected by pathogenic prions (PrPSc) and impaired the sprouting of neurites. In reconstructed networks of mature neurons, PrPSc-induced ROCK overactivation provoked synapse disconnection and dendrite/axon degeneration. This overactivation of ROCK also disturbed overall neurotransmitter-associated functions. Importantly, we demonstrated that beyond its impact on neuronal polarity ROCK overactivity favored the production of PrPSc through a ROCK-dependent control of 3-phosphoinositide-dependent kinase 1 (PDK1) activity. In non-infectious conditions, ROCK and PDK1 associated within a complex and ROCK phosphorylated PDK1, conferring basal activity to PDK1. In prion-infected neurons, exacerbated ROCK activity increased the pool of PDK1 molecules physically interacting with and phosphorylated by ROCK. ROCK-induced PDK1 overstimulation then canceled the neuroprotective α-cleavage of normal cellular prion protein PrPC by TACE α-secretase, which physiologically precludes PrPSc production. In prion-infected cells, inhibition of ROCK rescued neurite sprouting, preserved neuronal architecture, restored neuronal functions and reduced the amount of PrPSc. In mice challenged with prions, inhibition of ROCK also lowered brain PrPSc accumulation, reduced motor impairment and extended survival. We conclude that ROCK overactivation exerts a double detrimental effect in prion diseases by altering neuronal polarity and triggering PrPSc accumulation. Eventually ROCK emerges as therapeutic target to combat prion diseases. PMID:26241960

  17. Double-Edge Sword of Sustained ROCK Activation in Prion Diseases through Neuritogenesis Defects and Prion Accumulation.

    PubMed

    Alleaume-Butaux, Aurélie; Nicot, Simon; Pietri, Mathéa; Baudry, Anne; Dakowski, Caroline; Tixador, Philippe; Ardila-Osorio, Hector; Haeberlé, Anne-Marie; Bailly, Yannick; Peyrin, Jean-Michel; Launay, Jean-Marie; Kellermann, Odile; Schneider, Benoit

    2015-08-01

    In prion diseases, synapse dysfunction, axon retraction and loss of neuronal polarity precede neuronal death. The mechanisms driving such polarization defects, however, remain unclear. Here, we examined the contribution of RhoA-associated coiled-coil containing kinases (ROCK), key players in neuritogenesis, to prion diseases. We found that overactivation of ROCK signaling occurred in neuronal stem cells infected by pathogenic prions (PrPSc) and impaired the sprouting of neurites. In reconstructed networks of mature neurons, PrPSc-induced ROCK overactivation provoked synapse disconnection and dendrite/axon degeneration. This overactivation of ROCK also disturbed overall neurotransmitter-associated functions. Importantly, we demonstrated that beyond its impact on neuronal polarity ROCK overactivity favored the production of PrPSc through a ROCK-dependent control of 3-phosphoinositide-dependent kinase 1 (PDK1) activity. In non-infectious conditions, ROCK and PDK1 associated within a complex and ROCK phosphorylated PDK1, conferring basal activity to PDK1. In prion-infected neurons, exacerbated ROCK activity increased the pool of PDK1 molecules physically interacting with and phosphorylated by ROCK. ROCK-induced PDK1 overstimulation then canceled the neuroprotective α-cleavage of normal cellular prion protein PrPC by TACE α-secretase, which physiologically precludes PrPSc production. In prion-infected cells, inhibition of ROCK rescued neurite sprouting, preserved neuronal architecture, restored neuronal functions and reduced the amount of PrPSc. In mice challenged with prions, inhibition of ROCK also lowered brain PrPSc accumulation, reduced motor impairment and extended survival. We conclude that ROCK overactivation exerts a double detrimental effect in prion diseases by altering neuronal polarity and triggering PrPSc accumulation. Eventually ROCK emerges as therapeutic target to combat prion diseases.

  18. Double-Edge Sword of Sustained ROCK Activation in Prion Diseases through Neuritogenesis Defects and Prion Accumulation.

    PubMed

    Alleaume-Butaux, Aurélie; Nicot, Simon; Pietri, Mathéa; Baudry, Anne; Dakowski, Caroline; Tixador, Philippe; Ardila-Osorio, Hector; Haeberlé, Anne-Marie; Bailly, Yannick; Peyrin, Jean-Michel; Launay, Jean-Marie; Kellermann, Odile; Schneider, Benoit

    2015-08-01

    In prion diseases, synapse dysfunction, axon retraction and loss of neuronal polarity precede neuronal death. The mechanisms driving such polarization defects, however, remain unclear. Here, we examined the contribution of RhoA-associated coiled-coil containing kinases (ROCK), key players in neuritogenesis, to prion diseases. We found that overactivation of ROCK signaling occurred in neuronal stem cells infected by pathogenic prions (PrPSc) and impaired the sprouting of neurites. In reconstructed networks of mature neurons, PrPSc-induced ROCK overactivation provoked synapse disconnection and dendrite/axon degeneration. This overactivation of ROCK also disturbed overall neurotransmitter-associated functions. Importantly, we demonstrated that beyond its impact on neuronal polarity ROCK overactivity favored the production of PrPSc through a ROCK-dependent control of 3-phosphoinositide-dependent kinase 1 (PDK1) activity. In non-infectious conditions, ROCK and PDK1 associated within a complex and ROCK phosphorylated PDK1, conferring basal activity to PDK1. In prion-infected neurons, exacerbated ROCK activity increased the pool of PDK1 molecules physically interacting with and phosphorylated by ROCK. ROCK-induced PDK1 overstimulation then canceled the neuroprotective α-cleavage of normal cellular prion protein PrPC by TACE α-secretase, which physiologically precludes PrPSc production. In prion-infected cells, inhibition of ROCK rescued neurite sprouting, preserved neuronal architecture, restored neuronal functions and reduced the amount of PrPSc. In mice challenged with prions, inhibition of ROCK also lowered brain PrPSc accumulation, reduced motor impairment and extended survival. We conclude that ROCK overactivation exerts a double detrimental effect in prion diseases by altering neuronal polarity and triggering PrPSc accumulation. Eventually ROCK emerges as therapeutic target to combat prion diseases. PMID:26241960

  19. APPLICATIONS OF BOREHOLE-ACOUSTIC METHODS IN ROCK MECHANICS.

    USGS Publications Warehouse

    Paillet, Frederick L.

    1985-01-01

    Acoustic-logging methods using a considerable range of wavelengths and frequencies have proven very useful in the in situ characterization of deeply buried crystalline rocks. Seismic velocities are useful in investigating the moduli of unfractured rock, and in producing a continuous record of rock quality for comparison with discontinuous intervals of core. The considerable range of frequencies makes the investigation of scale effects possible in both fractured and unfractured rock. Several specific methods for the characterization of in situ permeability have been developed and verified in the field.

  20. Geometric Effect of Asperities on Shear Mechanism of Rock Joints

    NASA Astrophysics Data System (ADS)

    Fathi, Ali; Moradian, Zabihallah; Rivard, Patrice; Ballivy, Gérard; Boyd, Andrew J.

    2016-03-01

    Three-dimensional tracking of changes of asperities is one of the most important ways to illustrate shear mechanism of rock joints during testing. In this paper, the changes of the role of asperities during different stages of shearing are described by using a new methodology for the characterization of the asperities. The basis of the proposed method is the examination of the three-dimensional roughness of joint surfaces scanned before and after shear testing. By defining a concept named `tiny window', the geometric model of the joint surfaces is reconstructed. Tiny windows are expressed as a function of the x and y coordinates, the height (z coordinate), and the angle of a small area of the surface. Constant normal load (CNL) direct shear tests were conducted on replica joints and, by using the proposed method, the distribution and size of contact and damaged areas were identified. Image analysis of the surfaces was used to verify the results of the proposed method. The results indicated that the proposed method is suitable for determining the size and distribution of the contact and damaged areas at any shearing stage. The geometric properties of the tiny windows in the pre-peak, peak, post-peak softening, and residual shearing stages were investigated based on their angle and height. It was found that tiny windows that face the shear direction, especially the steepest ones, have a primary role in shearing. However, due to degradation of asperities at higher normal stresses and shear displacements, some of the tiny windows that do not initially face the shear direction also come in contact. It was also observed that tiny windows with different heights participate in the shearing process, not just the highest ones. Total contact area of the joint surfaces was considered as summation of just-in-contact areas and damaged areas. The results of the proposed method indicated that considering differences between just-in-contact areas and damaged areas provide useful

  1. Workshop on rock mechanics issues in repository design and performance assessment

    SciTech Connect

    1996-04-01

    The Center for Nuclear Waste Regulatory Analyses organized and hosted a workshop on ``Rock Mechanics Issues in Repository Design and Performance Assessment`` on behalf its sponsor the U.S. Nuclear Regulatory Commission (NRC). This workshop was held on September 19- 20, 1994 at the Holiday Inn Crowne Plaza, Rockville, Maryland. The objectives of the workshop were to stimulate exchange of technical information among parties actively investigating rock mechanics issues relevant to the proposed high-level waste repository at Yucca Mountain and identify/confirm rock mechanics issues important to repository design and performance assessment The workshop contained three technical sessions and two panel discussions. The participants included technical and research staffs representing the NRC and the Department of Energy and their contractors, as well as researchers from the academic, commercial, and international technical communities. These proceedings include most of the technical papers presented in the technical sessions and the transcripts for the two panel discussions. Selected papers have been indexed separately for inclusion the Energy Science and Technology Database.

  2. Mechanical Behaviour of Reservoir Rock Under Brine Saturation

    NASA Astrophysics Data System (ADS)

    Shukla, Richa; Ranjith, P. G.; Choi, S. K.; Haque, A.; Yellishetty, Mohan; Hong, Li

    2013-01-01

    Acoustic emissions (AE) and stress-strain curve analysis are well accepted ways of analysing crack propagation and monitoring the various failure stages (such as crack closure, crack initiation level during rock failure under compression) of rocks and rock-like materials. This paper presents details and results of experimental investigations conducted for characterizing the brittle failure processes induced in a rock due to monocyclic uniaxial compression on loading of two types of sandstone core samples saturated in NaCl brines of varying concentration (0, 2, 5, 10 and 15 % NaCl by weight). The two types of sandstone samples were saturated under vacuum for more than 45 days with the respective pore fluid to allow them to interact with the rocks. It was observed that the uniaxial compressive strength and stress-strain behaviour of the rock specimens changed with increasing NaCl concentration in the saturating fluid. The acoustic emission patterns also varied considerably for increasing ionic strength of the saturating brines. These observations can be attributed to the deposition of NaCl crystals in the rock's pore spaces as well some minor geo-chemical interactions between the rock minerals and the brine. The AE pattern variations could also be partly related to the higher conductivity of the ionic strength of the high-NaCl concentration brine as it is able to transfer more acoustic energy from the cracks to the AE sensors.

  3. Strain localisation in mechanically Layered Rocks, insights from numerical modelling

    NASA Astrophysics Data System (ADS)

    Le Pourhiet, L.; Huet, B.; Agard, P.; Labrousse, L.; Jolivet, L.; Yao, K.

    2012-09-01

    Small scale deformation in stratified rocks displays a large diversity of micro-structures, from the microscopic scale to the scale of orogens. We have designed a series of fully dynamic numerical simulations aimed at assessing which parameters control this structural diversity and which underlying mechanisms lead to strain localisation. The influence of stratification orientation on the occurrence and mode of strain localisation is tested by varying the initial dip of inherited layering versus the large scale imposed simple shear. The detailed study of the models indicates that (1) the results are length-scale independent, (2) the new shear zones are always compatible with the kinematics imposed at the boundary (3) micro-structures formed encompass the full diversity of micro-structures observed in the field and chiefly depend on the direction of the initial anisotropy versus shear direction, (4) depending on the orientation of the anisotropy, the layers may deform along subtractive or additive shear bands, (5) the deformation in anisotropic media results in non-lithostatic pressure values that are on the order of the deviatoric stress in the strong layers and (6) the introduction of brittle rheology is necessary to form localised shear bands in the ductile regime.

  4. Event triggered data acquisition in the Rock Mechanics Laboratory

    SciTech Connect

    Hardy, R.D.

    1993-03-01

    Increasing complexity of experiments coupled with limitations of the previously used computers required improvements in both hardware and software in the Rock Mechanics Laboratories. Increasing numbers of input channels and the need for better graphics could no longer be supplied by DATAVG, an existing software package for data acquisition and display written by D. J. Holcomb in 1983. After researching the market and trying several alternatives, no commercial program was found which met our needs. The previous version of DATAVG had the basic features needed but was tied to obsolete hardware. Memory limitations on the previously used PDP-11 made it impractical to upgrade the software further. With the advances in IBM compatible computers it is now desirable to use them as data recording platforms. With this information in mind, it was decided to write a new version of DATAVG which would take advantage of newer hardware. The new version had to support multiple graphic display windows and increased channel counts. It also had to be easier to use.

  5. Core-log integration for rock mechanics using borehole breakouts and rock strength experiments: Recent results from plate subduction margins

    NASA Astrophysics Data System (ADS)

    Saito, S.; Lin, W.

    2014-12-01

    Core-log integration has been applied for rock mechanics studies in scientific ocean drilling since 2007 in plate subduction margins such as Nankai Trough, Costa Rica margin, and Japan Trench. State of stress in subduction wedge is essential for controlling dynamics of plate boundary fault. One of the common methods to estimate stress state is analysis of borehole breakouts (drilling induced borehole wall compressive failures) recorded in borehole image logs to determine the maximum horizontal principal stress orientation. Borehole breakouts can also yield possible range of stress magnitude based on a rock compressive strength criterion. In this study, we constrained the stress magnitudes based on two different rock failure criteria, the Mohr-Coulomb (MC) criteria and the modified Wiebols-Cook (mWC) criteria. As the MC criterion is the same as that under unconfined compression state, only one rock parameter, unconfined compressive strength (UCS) is needed to constrain stress magnitudes. The mWC criterion needs the UCS, Poisson's ratio and internal frictional coefficient determined by triaxial compression experiments to take the intermediate principal stress effects on rock strength into consideration. We conducted various strength experiments on samples taken during IODP Expeditions 334/344 (Costa Rica Seismogenesis Project) to evaluate reliable method to estimate stress magnitudes. Our results show that the effects of the intermediate principal stress on the rock compressive failure occurred on a borehole wall is not negligible.

  6. Microstructures and flow mechanisms in regional metamorphic rocks of Japan

    NASA Astrophysics Data System (ADS)

    Toriumi, Mitsuhiro; Teruya, Jun; Masui, Megumi; Kuwahara, Hidesato

    1986-09-01

    A number of microstructural features indicate a difference in the dominant deformation mechanism between the higher temperature Ryoke and the lower temperature Sambagawa and Shimanto metamorphic belts of Japan. The microstructures of metacherts containing deformed radiolaria are divided into two types: in both the Sambagawa and Shimanto belts the quartz grains are tabular while in the Ryoke belt they are equiaxed. TEM studies of these metacherts revealed that the tabular grains contain abundant subboundaries consisting of large numbers of network dislocations and bowe-out dislocations, while the equiaxed grains contain no subboundaries and have low densities of dislocations which are not bowed-out. There is a corresponding difference in the textures (lattice preferred orientation of quartz): the Ryoke metacherts display randomly distributed c-axes of quartz, while the Sambagawa and Shimanto metacherts show conspicuous crossed girdle patterns with some asymmetry. There is a third difference between these regions: in the metacherts of the Ryoke metamorphic belt, the strain magnitudes determined from deformed radiolaria increase with increasing volume fraction of mica in the same metamorphic P and T conditions, while in the Sambagawa and the Shimanto metamorphic cherts the strain magnitudes decrease with increasing the mica fraction. These microstructures, textures, and rheological behaviours of quartz-mica rocks suggest a change of deformation mechanism between the lower temperature Sambagawa and Shimanto, and the higher temperature Ryoke metamorphic belts. Since random fabrics of c-axes of quartz are inconsistent with lattice rotation due to dislocation glide, the Ryoke metacherts may have deformed by pressure-solution.

  7. Geophysical analysis of rock glacier internal structure and implications for deformation mechanics

    NASA Astrophysics Data System (ADS)

    Florentine, C. E.; Skidmore, M. L.; Speece, M. A.; Link, C. A.; Locke, W. W.; Carr, C. G.; Shaw, C. A.

    2011-12-01

    An analysis of the internal composition and structure on the active portion of the Lone Peak Rock Glacier (LPRG), Madison range, southwest Montana revealed links between internal structure and surface topography. Seismic refraction surveys performed along transverse and longitudinal profiles corroborate borehole and excavation data by demonstrating a consistent and distinct transition from unconsolidated (unfrozen) surface debris (2-3 m thick) to a consolidated (frozen) subsurface material. Refraction velocities for the seismic survey transects were relatively consistent along their length with 400 m s-1 for the upper layer detected, and 3500 m s-1 for the lower layer detected at a depth of 2-3 m. This second velocity of 3500 m s-1 is consistent with other observed refraction velocities for ice. Ground penetrating radar (GPR) data along similar longitudinal and transverse profiles identified up-slope dipping structures to a depth of ~10 m, consistent with layering of materials with contrasting radar properties within the ice-rock unit. The GPR data is interpreted as a sequence of alternating debris-poor and debris-rich layers which dip upslope toward the rock-glacier headwall along the longitudinal profile, and which show correspondence with transverse ridges at the surface. The presence of fault bounded blocks (i.e. structural horses) detected in the longitudinal GPR data suggests passive roof duplex thrust faulting, in which the roof sequence - unconsolidated (unfrozen) debris - has not been displaced toward the foreland (down glacier), but has been underthrust by the duplex. Transverse ridges commonly characterize rock glacier surfaces in a range of locations worldwide. Approximately one third of 383 rock glaciers inventoried in southwest Montana demonstrate pronounced transverse ridges. It has previously been suggested that transverse ridges are the product of thrusting, which is caused by compressive flow in rock glaciers. Thrusting however has not been

  8. U.S. National Committee for Rock Mechanics; and Conceptual model of fluid infiltration in fractured media. Project summary, July 28, 1997--July 27, 1998

    SciTech Connect

    1998-09-01

    The title describes the two tasks summarized in this report. The remainder of the report contains information on meetings held or to be held on the subjects. The US National Committee for Rock Mechanics (USNC/RM) provides for US participation in international activities in rock mechanics, principally through adherence to the International Society for Rock Mechanics (ISRM). It also keeps the US rock mechanics community informed about new programs directed toward major areas of national concern in which rock mechanics problems represent critical or limiting factors, such as energy resources, excavation, underground storage and waste disposal, and reactor siting. The committee also guides or produces advisory studies and reports on problem areas in rock mechanics. A new panel under the auspices of the US National Committee for Rock Mechanics has been appointed to conduct a study on Conceptual Models of Fluid Infiltration in Fractured Media. The study has health and environmental applications related to the underground flow of pollutants through fractured rock in and around mines and waste repositories. Support of the study has been received from the US Nuclear Regulatory Commission and the Department of Energy`s Yucca Mountain Project Office. The new study builds on the success of a recent USNC/RM report entitled Rock Fractures and Fluid Flow: Contemporary Understanding and Applications (National Academy Press, 1996, 551 pp.). A summary of the new study is provided.

  9. Load transfer mechanisms in rock sockets and anchors. Final report

    SciTech Connect

    Pease, K.A.; Kulhawy, F.H.

    1984-11-01

    This study presents a comprehensive analysis of rock socket and anchor behavior, which includes the failure mode, capacity, and deformations. The methods of analysis were obtained from a combination of original concepts and summaries of preexisting methods. The original concepts are based on both theoretical considerations and observations of actual socket behavior. The data used for both analytical and verification purposes were obtained from published sources. The results show that rock sockets and anchors can fail by any one of four modes, including: tensile failure of the tendons, pullout of the tendons from the grout, grout-rock interface slip, and rock mass uplift. Only the latter two are geotechnical problems investigated in detail.

  10. Statistical mechanics of fragmentation processes of ice and rock bodies

    NASA Astrophysics Data System (ADS)

    Bashkirov, A. G.; Vityazev, A. V.

    1996-09-01

    It is a well-known experimental fact that impact fragmentation, specifically of ice and rock bodies, causes a two-step ("knee"-shaped) power distribution of fragment masses with exponent values within the limits -4 and -1.5 (here and henceforth the differential distribution is borne in mind). A new theoretical approach is proposed to determine the exponent values, a minimal fracture mass, and properties of the knee. As a basis for construction of non-equilibrium statistical mechanics of condensed matter fragmentation the maximum-entropy variational principle is used. In contrast to the usual approach founded on the Boltzmann entropy the more general Tsallis entropy allowing stationary solutions not only in the exponential Boltzmann-Gibbs form but in the form of the power (fractal) law distribution as well is invoked. Relying on the analysis of a lot of published experiments a parameter β is introduced to describe an inhomogeneous distribution of the impact energy over the target. It varies from 0 (for an utterly inhomogeneous distribution of the impact energy) to 1 (for a homogeneous distribution). The lower limit of fragment masses is defined as a characteristic fragment mass for which the energy of fragment formation is minimal. This mass value depends crucially on the value of β. It is shown that for β≪1 only small fragments can be formed, and the maximal permitted fragment (of mass m1) is the upper boundary of the first stage of the fracture process and the point where the knee takes place. The second stage may be realized after a homogeneous redistribution of the remainder of the impact energy over the remainder of the target (when β→1). Here, the formation of great fragments is permitted only and the smallest of them (of mass m2) determines a lower boundary of the second stage. Different forms of the knee can be observed depending on relations between m1 and m2.

  11. Mechanical Behavior of the Near-field Host Rock Surrounding Excavations

    SciTech Connect

    Kelkar, Sharad M.; Stauffer, Philip H.; Robinson, Bruce Alan

    2015-01-09

    This report is being prepared under the FY14 activity FT-14LA0818069, Mechanical and Hydrological Behavior of the Near-Field Host Rock Surrounding Excavations, and fulfills the Los Alamos National Laboratory deliverable M4FT-14LA08180610, which in PICS:NE is titled “Draft report, Test Plan for Mechanical and Hydrological Behavior of the Near-field Host Rock Surrounding Excavations.” Since the report is an intermediate deliverable intended as input to the eventual test plan for this test, rather than being an actual test plan, the activity title is used as the title of this document to avoid confusion as to the contents in the report. This report summarizes efforts to simulate mechanical processes occurring within a hypothetical high-level waste (HLW) repository in bedded salt. The report summarizes work completed since the last project deliverable, “Coupled model for heat and water transport in a high level waste repository in salt “, a Level 2 milestone submitted to DOE in September 2013 (Stauffer et al., 2013).

  12. Is rock slope instability in high-mountain systems driven by topo-climatic, paraglacial or rock mechanical factors? - A question of scale!

    NASA Astrophysics Data System (ADS)

    Messenzehl, Karoline; Dikau, Richard

    2016-04-01

    Due to the emergent and (often non-linear) complex nature of mountain systems the key small-scale system properties responsible for rock slope instability contrast to those being dominant at larger spatial scales. This geomorphic system behaviour has major epistemological consequences for the study of rockfalls and associated form-process-relationships. As each scale requires its own scientific explanation, we cannot simply upscale bedrock-scale findings and, in turn, we cannot downscale the valley-scale knowledge to smaller phenomena. Here, we present a multi-scale study from the Turtmann Valley (Swiss Alps), that addresses rock slope properties at three different geomorphic levels: (i) regional valley scale, (ii) the hillslope scale and (iii) the bedrock scale. Using this hierarchical approach, we aim to understand the key properties of high-mountain systems responsible for rockfall initiation with respect to the resulting form-process-relationship at each scale. (i) At the valley scale (110 km2) rock slope instability was evaluated using a GIS-based modelling approach. Topo-climatic parameters, i.e. the permafrost distribution and the time since deglaciation after LGM were found to be the key variables causative for the regional-scale bedrock erosion and the storage of 62.3 - 65.3 x 106 m3 rockfall sediments in the hanging valleys (Messenzehl et al. 2015). (ii) At the hillslope scale (0.03 km2) geotechnical scanline surveys of 16 rock slopes and one-year rock temperature data of 25 ibuttons reveal that the local rockfall activity and the resulting deposition of individual talus slope landforms is mainly controlled by the specific rock mass strength with respect to the slope aspect, than being a paraglacial reaction. Permafrost might be only of secondary importance for the present-day rock mechanical state as geophysical surveys disprove the existence of frozen bedrock below 2600 m asl. (Messenzehl & Draebing 2015). (iii) At the bedrock scale (0.01 mm - 10 m) the

  13. Determination of indium in standard rocks by neutron activation analysis.

    PubMed

    Johansen, O; Steinnes, E

    1966-08-01

    A rapid neutron activation method for the determination of indium in rocks, based on 54 min (116m)In, is described. The method has been applied to a series of geochemical standards including granite G-1 and diabase W-1. The precision is better than +/- 5% for samples containing more than 5 x 10(-10)g indium. Good agreement with previously published values for G-1 and W-1 has been obtained.

  14. Determination of indium in standard rocks by neutron activation analysis.

    PubMed

    Johansen, O; Steinnes, E

    1966-08-01

    A rapid neutron activation method for the determination of indium in rocks, based on 54 min (116m)In, is described. The method has been applied to a series of geochemical standards including granite G-1 and diabase W-1. The precision is better than +/- 5% for samples containing more than 5 x 10(-10)g indium. Good agreement with previously published values for G-1 and W-1 has been obtained. PMID:18959988

  15. Mechanically Activated Ion Channels

    PubMed Central

    Ranade, Sanjeev S.; Syeda, Ruhma; Patapoutian, Ardem

    2015-01-01

    Mechanotransduction, the conversion of physical forces into biochemical signals, is an essential component of numerous physiological processes including not only conscious senses of touch and hearing, but also unconscious senses such as blood pressure regulation. Mechanically activated (MA) ion channels have been proposed as sensors of physical force, but the identity of these channels and an understanding of how mechanical force is transduced has remained elusive. A number of recent studies on previously known ion channels along with the identification of novel MA ion channels have greatly transformed our understanding of touch and hearing in both vertebrates and invertebrates. Here, we present an updated review of eukaryotic ion channel families that have been implicated in mechanotransduction processes and evaluate the qualifications of the candidate genes according to specified criteria. We then discuss the proposed gating models for MA ion channels and highlight recent structural studies of mechanosensitive potassium channels. PMID:26402601

  16. Experimental Researches on Hydro-Mechanical Properties of Altered Rock Under Confining Pressures

    NASA Astrophysics Data System (ADS)

    Wang, H. L.; Xu, W. Y.; Shao, J. F.

    2014-03-01

    Altered rock, as the abutment materials of Xiaowan Hydropower Station in China, is a kind of geological defective rock mass. It is loosely structured and its strength is low, with some development of pores and cavities. Research on the hydro-mechanical coupling of the altered rock are of important significance to hydropower projects. In this study, the advanced fully automatic triaxial fluid flow-rheological test servo system is employed to study the hydro-mechanical coupling characteristics of the altered rock, and the water pressures and confining pressures in the laboratory tests are set to simulate the conditions of excavation and impoundment of Xiaowan Hydropower Station. Based on the test results, the stress-strain laws of the rock specimens under the effect of complete hydro-mechanical coupling, as well as the lateral strain and volumetric strain characteristics, are studied. The fluid flow laws of the rock specimens and the effects of the confining pressures on the fluid flow are analyzed. The fluid flow failure characteristic under the effect of the complete hydro-mechanical coupling is discussed. The research achievements show that with the change of the stress states, the permeability of the rock also changes, and the permeability evolution shows the phase characteristic during the process of stress and strain. The impacts of the confining pressures on the strength and deformation and permeability of the altered rock are obvious. The failure behaviours of the rock specimens under the effect of coupling relates to the confining pressures, including two kinds of splitting failure and shear failure. The fluid flow failure characteristic of the rock specimens depend upon the initiation, growth and coalesce of micro-cracks, heterogeneity, confining pressures and properties of the rock.

  17. Rock mass mechanical property estimations for the Yucca Mountain Site Characterization Project; Yucca Mountain Site Characterization Project

    SciTech Connect

    Lin, M.; Hardy, M.P.; Bauer, S.J.

    1993-06-01

    Rock mass mechanical properties are important in the design of drifts and ramps. These properties are used in evaluations of the impacts of thermomechanical loading of potential host rock within the Yucca Mountain Site Characterization Project. Representative intact rock and joint mechanical properties were selected for welded and nonwelded tuffs from the currently available data sources. Rock mass qualities were then estimated using both the Norwegian Geotechnical Institute (Q) and Geomechanics Rating (RMR) systems. Rock mass mechanical properties were developed based on estimates of rock mass quality, the current knowledge of intact properties, and fracture/joint characteristics. Empirical relationships developed to correlate the rock mass quality indices and the rock mass mechanical properties were then used to estimate the range of rock mass mechanical properties.

  18. The three-dimension model for the rock-breaking mechanism of disc cutter and analysis of rock-breaking forces

    NASA Astrophysics Data System (ADS)

    Zhang, Zhao-Huang; Sun, Fei

    2012-06-01

    To study the rock deformation with three-dimensional model under rolling forces of disc cutter, by carrying out the circular-grooving test with disc cutter rolling around on the rock, the rock mechanical behavior under rolling disc cutter is studied, the mechanical model of disc cutter rolling around the groove is established, and the theory of single-point and double-angle variables is proposed. Based on this theory, the physics equations and geometric equations of rock mechanical behavior under disc cutters of tunnel boring machine (TBM) are studied, and then the balance equations of interactive forces between disc cutter and rock are established. Accordingly, formulas about normal force, rolling force and side force of a disc cutter are derived, and their validity is studied by tests. Therefore, a new method and theory is proposed to study rock-breaking mechanism of disc cutters.

  19. Heterogeneities of mechanical properties in potential geothermal reservoir rocks of the North German Basin

    NASA Astrophysics Data System (ADS)

    Reyer, D.; Philipp, S. L.

    2012-04-01

    Heterogeneous rock properties in terms of layering and complex infrastructure of fault zones are typical phenomena in sedimentary basins such as the North German Basin. To be able to model reservoir stimulation in layered stratifications and to better adapt the drilling strategy to the rock mechanical conditions it is important to have knowledge about the effects of heterogeneous rock properties on fracture propagation and fault zone infrastructure for typical sedimentary reservoir rocks in the North German Basin. Therefore we aim at quantifying these properties by performing structural geological field studies in outcrop analogues combined with laboratory analyses. The field studies in Rotliegend sandstones (Lower Permian), the sandstones of the Middle Bunter (Lower Triassic) and the sandstones of the Upper Keuper (Upper Triassic) focus on 1) host rock fracture systems and 2) fault zone infrastructure. We analyse quantitatively the dimension, geometry, persistence and connectivity of fracture systems separately for host rocks and fault damage zones. The results show that in rocks with distinctive layering (sandstones and shales) natural fractures are often restricted to individual layers, that is, they are stratabound. The probability of fracture arrest seems to depend on the stiffness contrast between the two layers and on the thickness of the softer layer. The field studies are complemented by systematic sampling to obtain mechanical property variations caused by the layering. For the samples we measure the parameters Young's modulus, compressive and tensile strengths, elastic strain energy, density and porosity. The results show that the mechanical properties vary considerably and many samples are clearly anisotropic. That is, samples taken perpendicular to layering commonly have higher strengths but lower stiffnesses than those taken parallel to layering. We combine the results of laboratory analyses and field measurements to specify the mechanical

  20. Mechanical Characteristics of rocks cored from Hanging Wall of Chelungpu Fault

    NASA Astrophysics Data System (ADS)

    Lu, C. Y.; Chen, C. W.; Hu, J. C.; Tsai, L. S.; Lin, M. L.; Jeng, F. S.

    2005-12-01

    Representatives mechanical parameters of the strata involved in fault movements are essentially needed when conducting numerical simulation of fault movements. To study the mechanical characteristic of the rocks, to conduct the subsequent numberical analyses, and to interpret the Chelungpu fault movements, this research systematically studied the mechanical properties of the rocks cored from the TCDP program. Accordingly, the samples sampled from the cores are subjected to a series of mechanical experiments, including stress-path controlled pure shear tests to study their physical properties as well as their strengths and deformability with elastic and plastic strained being distinguished. Meanwhile, temperature factor are also conducted so as to study its influence on the rocks. It was found that the rocks, with a depth ranging from 450 to 1300m, mainly comprise of silt stone, fossil-riched sandstone and sandstone, with a porosities of 2%, 6% and 15% and uniaxial compressive strengths of 67~73 MPa, 61~65 MPa and 8~11 MPa, respectively. Accordingly, we can find that the strength and deformation of rocks relate to their porosities. According to the pure shear path test results, it was also found that the behavior of the last two types rocks, including the non-linear elastic deformation, plastic strain locking and the elastic strain, was coupled with shear stress. As to the temperature , its influence on the rocks was found to be not significant. On the other hand, the experimental results indicate that the Drucker-Prager failure criteria can describe the failure envelope line of these three type rocks. The strength of these rocks is independent with stress-path. Finally, the constitutive parameters of these sandstones were obtained, which enables realistic prediction the deformational behavior of the rocks in the near future.

  1. Rock mechanics issues and research needs in the disposal of wastes in hydraulic fractures

    SciTech Connect

    Doe, T.W.; McClain, W.C.

    1984-07-01

    The proposed rock mechanics studies outlined in this document are designed to answer the basic questions concerning hydraulic fracturing for waste disposal. These questions are: (1) how can containment be assured for Oak Ridge or other sites; and (2) what is the capacity of a site. The suggested rock mechanics program consists of four major tasks: (1) numerical modeling, (2) laboratory testing, (3) field testing, and (4) monitoring. These tasks are described.

  2. Event triggered data acquisition in the Rock Mechanics Laboratory upgrades and revisions

    SciTech Connect

    Hardy, R.D.

    1997-06-01

    This paper describes updates and revisions to the data acquisition computer program DATAVG which has served as the basic data collection system for the Sandia National Laboratories Geomechanics Department, Rock Mechanics Laboratory since late 1992. DATAVG was first described in Event Triggered Data Acquisition in the Rock Mechanics Laboratory, [Hardy, 1993]. DATAVG has been modified to incorporate numerous user-requested enhancements and a few bug fixes. In this paper these changes to DATAVG are described.

  3. Correlating P-wave Velocity with the Physico-Mechanical Properties of Different Rocks

    NASA Astrophysics Data System (ADS)

    Khandelwal, Manoj

    2013-04-01

    In mining and civil engineering projects, physico-mechanical properties of the rock affect both the project design and the construction operation. Determination of various physico-mechanical properties of rocks is expensive and time consuming, and sometimes it is very difficult to get cores to perform direct tests to evaluate the rock mass. The purpose of this work is to investigate the relationships between the different physico-mechanical properties of the various rock types with the P-wave velocity. Measurement of P-wave velocity is relatively cheap, non-destructive and easy to carry out. In this study, representative rock mass samples of igneous, sedimentary, and metamorphic rocks were collected from the different locations of India to obtain an empirical relation between P-wave velocity and uniaxial compressive strength, tensile strength, punch shear, density, slake durability index, Young's modulus, Poisson's ratio, impact strength index and Schmidt hammer rebound number. A very strong correlation was found between the P-wave velocity and different physico-mechanical properties of various rock types with very high coefficients of determination. To check the sensitivity of the empirical equations, Students t test was also performed, which confirmed the validity of the proposed correlations.

  4. The mechanism and dynamics of rock fracture upon mechanical impact and electric discharge

    NASA Astrophysics Data System (ADS)

    Vettegren, V. I.; Kuksenko, V. S.; Shcherbakov, I. P.

    2016-09-01

    The mechanism and dynamics of the deformation and fracture of quartz, granite, and marble samples under the striker blow on their surface and electric discharge inside them are studied by the fractoluminescence (FL), electromagnetic (EME), and acoustic emission (AE) methods with 10-ns resolution. The impact excites a forced deformation wave with a velocity within 0.8 to 2 km/s depending on the mineral. The atomic bonds rupture and microcracks are formed at the nodes of the wave, which leads to the emergence of the FL flashes and disruption of the time dependences of EME. Based on the intensity of the flashes, the dimensions of microcracks are estimated to vary from 2 to 70 µm depending on the mineral. In turn, the emergence of microcracks initiates additional deformation waves.The discharge inside the studied samples excites a pressure shock wave which transforms into the tension wave after reflection from the surface. According to the analysis of FL spectra, this leads to the breakdown of the rocks into positively charged ions and electrons. The shock wave velocity in granites is measured at 4.8 km/s, which is close to the velocity of the longitudinal acoustic vibrations ~5 km/s. The microcracks in the rock have not enough time to form with this loading velocity. It is supposed that the shock wave stretches the deformed interatomic bonds at the dislocation nuclei in the crystal lattices of the minerals up to their breakdown into positively charged ions.

  5. Hydro-mechanically coupled modelling of deep-seated rock slides in the surroundings of reservoirs

    NASA Astrophysics Data System (ADS)

    Lechner, Heidrun; Preh, Alexander; Zangerl, Christian

    2016-04-01

    In order to enhance the understanding of the behaviour of deep-seated rock slides in the surroundings of large dam reservoirs, this study concentrates on failure mechanisms, deformation processes and the ability of self-stabilisation of rock slides influenced by reservoirs. Particular focus is put on internal rock mass deformations, progressive topographical slope changes due to reservoir impoundment and shear displacements along the basal shear zone in relation to its shear strength properties. In this study, a two-dimensional numerical rock slide model is designed by means of the Universal Distinct Element Code UDEC and investigated concerning different groundwater flow scenarios. These include: (i) a completely drained rock slide model, (ii) a model with fully saturated rock mass below an inclined groundwater table and (iii) a saturated groundwater model with a reservoir at the slope toe. Slope displacements initiate when the shear strength properties of the basal shear zone are at or below the critical parameters for the limit-equilibrium state and continue until a numerical equilibrium is reached due to deformation- and displacement-based geometrical changes. The study focuses on the influence of a reservoir at the toe of a rock slide and tries to evaluate the degree of displacement which is needed for a re-stabilisation in relation to the geometrical characteristics of the rock slide. Besides, challenges and limitations of applied distinct element methods to simulate large strain and displacements of deep-seated rock slides are discussed. The ongoing study will help to understand the deformation behaviour of deep-seated pre-existing rock slides in fractured rock mass during initial impounding and will be part of a hazard assessment for large reservoirs.

  6. Deformation mechanisms in a coal mine roadway in extremely swelling soft rock.

    PubMed

    Li, Qinghai; Shi, Weiping; Yang, Renshu

    2016-01-01

    The problem of roadway support in swelling soft rock was one of the challenging problems during mining. For most geological conditions, combinations of two or more supporting approaches could meet the requirements of most roadways; however, in extremely swelling soft rock, combined approaches even could not control large deformations. The purpose of this work was to probe the roadway deformation mechanisms in extremely swelling soft rock. Based on the main return air-way in a coal mine, deformation monitoring and geomechanical analysis were conducted, as well as plastic zone mechanical model was analysed. Results indicated that this soft rock was potentially very swelling. When the ground stress acted alone, the support strength needed in situ was not too large and combined supporting approaches could meet this requirement; however, when this potential released, the roadway would undergo permanent deformation. When the loose zone reached 3 m within surrounding rock, remote stress p ∞ and supporting stress P presented a linear relationship. Namely, the greater the swelling stress, the more difficult it would be in roadway supporting. So in this extremely swelling soft rock, a better way to control roadway deformation was to control the releasing of surrounding rock's swelling potential.

  7. Assessment of rock wool as support material for on-site sanitation: hydrodynamic and mechanical characterization.

    PubMed

    Wanko, Adrien; Laurent, Julien; Bois, Paul; Mosé, Robert; Wagner-Kocher, Christiane; Bahlouli, Nadia; Tiffay, Serge; Braun, Bouke; Provo kluit, Pieter-Willem

    2016-01-01

    This study proposes mechanical and hydrodynamic characterization of rock wool used as support material in compact filter. A double-pronged approach, based on experimental simulation of various physical states of this material was done. First of all a scanning electron microscopy observation allows to highlight the fibrous network structure, the fibres sizing distribution and the atomic absorption spectrum. The material was essentially lacunar with 97 ± 2% of void space. Static compression tests on variably saturated rock wool samples provide the fact that the strain/stress behaviours depend on both the sample conditioning and the saturation level. Results showed that water exerts plastifying effect on mechanical behaviour of rock wool. The load-displacement curves and drainage evolution under different water saturation levels allowed exhibiting hydraulic retention capacities under stress. Finally, several tracer experiments on rock wool column considering continuous and batch feeding flow regime allowed: (i) to determine the flow model for each test case and the implications for water dynamic in rock wool medium, (ii) to assess the rock wool double porosity and discuss its advantages for wastewater treatment, (iii) to analyse the benefits effect for water treatment when the high level of rock wool hydric retention was associated with the plug-flow effect, and (iv) to discuss the practical contributions for compact filter conception and management.

  8. Deformation mechanisms in a coal mine roadway in extremely swelling soft rock.

    PubMed

    Li, Qinghai; Shi, Weiping; Yang, Renshu

    2016-01-01

    The problem of roadway support in swelling soft rock was one of the challenging problems during mining. For most geological conditions, combinations of two or more supporting approaches could meet the requirements of most roadways; however, in extremely swelling soft rock, combined approaches even could not control large deformations. The purpose of this work was to probe the roadway deformation mechanisms in extremely swelling soft rock. Based on the main return air-way in a coal mine, deformation monitoring and geomechanical analysis were conducted, as well as plastic zone mechanical model was analysed. Results indicated that this soft rock was potentially very swelling. When the ground stress acted alone, the support strength needed in situ was not too large and combined supporting approaches could meet this requirement; however, when this potential released, the roadway would undergo permanent deformation. When the loose zone reached 3 m within surrounding rock, remote stress p ∞ and supporting stress P presented a linear relationship. Namely, the greater the swelling stress, the more difficult it would be in roadway supporting. So in this extremely swelling soft rock, a better way to control roadway deformation was to control the releasing of surrounding rock's swelling potential. PMID:27547684

  9. Influence of Water Content on the Mechanical Properties of an Argillaceous Swelling Rock

    NASA Astrophysics Data System (ADS)

    Vergara, Maximiliano R.; Triantafyllidis, Theodoros

    2016-07-01

    This work presents the results of an experimental investigation aimed to study the effect of water on the mechanical properties of a partially saturated argillaceous swelling rock. The mineralogical composition of the rock, in particular the portion of swelling clays, was determined with X-ray diffraction. The water retention curve was estimated using a dew-point potential meter and the swelling behavior of the studied rock was examined by unconfined and oedometric swelling tests. The influence of water on the rock mechanical properties was assessed by means of triaxial tests. The experimental results indicate a strong decrease of strength and stiffness with increasing saturation or decreasing suction. This occurs only within a certain range of saturation. Degradation of the rock properties can be expected for small increments in the water content within this range. At low suction and close to the air-entry value, the stiffness remained constant. As the rock desaturates, the strength and stiffness increase approaching constant values. For suction greater than about 76 MPa, low increase of strength and stiffness was observed. The specimens in the swelling tests reached a saturation degree of 70 % which corresponds to a decrease of strength and stiffness of approximately 80 %. Rock swelling occurring simultaneously with reduction of strength and stiffness, increases deformations and it is an important issue for the stability of excavations.

  10. ACOUSTICAL IMAGING AND MECHANICAL PROPERTIES OF SOFT ROCK AND MARINE SEDIMENTS

    SciTech Connect

    Thurman E. Scott, Jr., Ph.D.; Younane Abousleiman, Ph.D.; Musharraf Zaman, Ph.D., P.E.

    2001-01-31

    During this phase of the project the research team concentrated on acquisition of acoustic emission data from the high porosity rock samples. The initial experiments indicated that the acoustic emission activity from high porosity Danian chalk were of a very low amplitude. Even though the sample underwent yielding and significant plastic deformation the sample did not generate significant AE activity. This was somewhat surprising. These initial results call into question the validity of attempting to locate AE activity in this weak rock type. As a result the testing program was slightly altered to include measuring the acoustic emission activity from many of the rock types listed in the research program. The preliminary experimental results indicate that AE activity in the sandstones is much higher than in the carbonate rocks (i.e., the chalks and limestones). This observation may be particularly important for planning microseismic imaging of reservoir rocks in the field environment. The preliminary results suggest that microseismic imaging of reservoir rock from acoustic emission activity generated from matrix deformation (during compaction and subsidence) would be extremely difficult to accomplish.

  11. The migration law of overlay rock and coal in deeply inclined coal seam with fully mechanized top coal caving.

    PubMed

    Liu, Jian; Chen, Shan-Le; Wang, Hua-Jun; Li, Yu-Cheng; Geng, Xiaowei

    2015-07-01

    In a mine area, some environment geotechnics problems always occure, induced by mined-out region such as the subsidence and cracks at ground level, deformation and destruction of buildings, landslides destruction of water resources and the ecological environment. In order to research the migration of surrounding rock and coal in steeply inclined super high seams which used fully mechanized top coal caving, a working face of a certain mine was made as an example, analyzed the migration law of the overlay rock and coal under different caving ratio of fully mechanized top coal caving with numerical simulation analysis. The results suggest that the laws of overlay rock deformation caused by deeply inclined coal seam were different from horizontal coal seam. On the inclined direction, with an increase of dip angle and caving ratio, the vertical displacement of overlay rock and coal became greater, the asymmetric phenomenon of vertical displacement became obvious. On the trend direction, active region and transition region in goaf became smaller along with the increase of mining and caving ratio. On the contrary, the stable region area became greater. Therefore, there was an essential difference between the mechanism of surface movement deformation with deeply inclined coal seam and that with horizontal coal seam. PMID:26387357

  12. The migration law of overlay rock and coal in deeply inclined coal seam with fully mechanized top coal caving.

    PubMed

    Liu, Jian; Chen, Shan-Le; Wang, Hua-Jun; Li, Yu-Cheng; Geng, Xiaowei

    2015-07-01

    In a mine area, some environment geotechnics problems always occure, induced by mined-out region such as the subsidence and cracks at ground level, deformation and destruction of buildings, landslides destruction of water resources and the ecological environment. In order to research the migration of surrounding rock and coal in steeply inclined super high seams which used fully mechanized top coal caving, a working face of a certain mine was made as an example, analyzed the migration law of the overlay rock and coal under different caving ratio of fully mechanized top coal caving with numerical simulation analysis. The results suggest that the laws of overlay rock deformation caused by deeply inclined coal seam were different from horizontal coal seam. On the inclined direction, with an increase of dip angle and caving ratio, the vertical displacement of overlay rock and coal became greater, the asymmetric phenomenon of vertical displacement became obvious. On the trend direction, active region and transition region in goaf became smaller along with the increase of mining and caving ratio. On the contrary, the stable region area became greater. Therefore, there was an essential difference between the mechanism of surface movement deformation with deeply inclined coal seam and that with horizontal coal seam.

  13. Interactive evolution concept for analyzing a rock salt cavern under cyclic thermo-mechanical loading

    NASA Astrophysics Data System (ADS)

    König, Diethard; Mahmoudi, Elham; Khaledi, Kavan; von Blumenthal, Achim; Schanz, Tom

    2016-04-01

    The excess electricity produced by renewable energy sources available during off-peak periods of consumption can be used e.g. to produce and compress hydrogen or to compress air. Afterwards the pressurized gas is stored in the rock salt cavities. During this process, thermo-mechanical cyclic loading is applied to the rock salt surrounding the cavern. Compared to the operation of conventional storage caverns in rock salt the frequencies of filling and discharging cycles and therefore the thermo-mechanical loading cycles are much higher, e.g. daily or weekly compared to seasonally or yearly. The stress strain behavior of rock salt as well as the deformation behavior and the stability of caverns in rock salt under such loading conditions are unknown. To overcome this, existing experimental studies have to be supplemented by exploring the behavior of rock salt under combined thermo-mechanical cyclic loading. Existing constitutive relations have to be extended to cover degradation of rock salt under thermo-mechanical cyclic loading. At least the complex system of a cavern in rock salt under these loading conditions has to be analyzed by numerical modeling taking into account the uncertainties due to limited access in large depth to investigate material composition and properties. An interactive evolution concept is presented to link the different components of such a study - experimental modeling, constitutive modeling and numerical modeling. A triaxial experimental setup is designed to characterize the cyclic thermo-mechanical behavior of rock salt. The imposed boundary conditions in the experimental setup are assumed to be similar to the stress state obtained from a full-scale numerical simulation. The computational model relies primarily on the governing constitutive model for predicting the behavior of rock salt cavity. Hence, a sophisticated elasto-viscoplastic creep constitutive model is developed to take into account the dilatancy and damage progress, as well as

  14. Sulfate-dependent Anaerobic Oxidation of Methane as a Generation Mechanism for Calcite Cap Rock in Gulf Coast Salt Domes

    NASA Astrophysics Data System (ADS)

    Caesar, K. H.; Kyle, R.; Lyons, T. W.; Loyd, S. J.

    2015-12-01

    Gulf Coast salt domes, specifically their calcite cap rocks, have been widely recognized for their association with significant reserves of crude oil and natural gas. However, the specific microbial reactions that facilitate the precipitation of these cap rocks are still largely unknown. Insight into the mineralization mechanism(s) can be obtained from the specific geochemical signatures recorded in these structures. Gulf Coast cap rocks contain carbonate and sulfur minerals that exhibit variable carbon (d13C) and sulfur isotope (δ34S) signatures. Calcite d13C values are isotopically depleted and show a large range of values from -1 to -52‰, reflecting a mixture of various carbon sources including a substantial methane component. These depleted carbon isotope compositions combined with the presence of abundant sulfide minerals in cap rocks have led to interpretations that invoke microbial sulfate reduction as an important carbonate mineral-yielding process in salt dome environments. Sulfur isotope data from carbonate-associated sulfate (CAS: trace sulfate incorporated within the carbonate mineral crystal lattice) provide a more direct proxy for aqueous sulfate in salt dome systems and may provide a means to directly fingerprint ancient sulfate reduction. We find CAS sulfur isotope compositions (δ34SCAS) significantly greater than those of the precursor Jurassic sulfate-salt deposits (which exhibit δ34S values of ~ +15‰). This implies that cap rock carbonate generation occurred via microbial sulfate reduction under closed-system conditions. The co-occurrence of depleted carbonate d13C values (< ~30‰) and the enriched δ34SCAS values are evidence for sulfate-dependent anaerobic oxidation of methane (AOM). AOM, which has been shown to yield extensive seafloor carbonate authigenesis, is also potentially partly responsible for the carbonate minerals of the Gulf Coast calcite cap rocks through concomitant production of alkalinity. Collectively, these data shed

  15. Critical review of the state-of-the-art of fracture mechanics with emphasis on layered rocks

    SciTech Connect

    Kuruppu, M.D.; Cheng, K.P.; Edl, J.N. Jr.

    1983-07-01

    Results are presented of a literature survey of over 70 pertinent publications and critical reviews of fracture mechanics emphasizing the fracture behavior of layered rocks. Historical perspective, fracture mechanisms, linear and nonlinear fracture mechanics, energy theories, ductile and brittle fractures, process regions, specific work of fracture, J-integrals, failure theories, static and dynamic fractures, rock fracture mechanics, fracture toughness of layered rocks (e.g., oil shale), experimental and numerical methods are reviewed and discussed. Innovative and promising methods tailored for the fracture mechanics of layered rocks are recommended.

  16. Earthquake-induced collapse mechanism of two types of dangerous rock masses

    NASA Astrophysics Data System (ADS)

    Wang, Wei; Yuan, Wei; Wang, Qizhi; Xue, Kang

    2016-06-01

    As the economy of China develops, an increasing number of key traffic projects have been undertaken in the west of China, where there are high, steep rock slopes. The collapse of dangerous rock masses, especially following a strong earthquake, is one of common geological disasters known in rock slope engineering. Therefore, it is important to study the collapse mechanism of dangerous rock masses induced by an earthquake and the analysis approach of its stability. This study provides a simple and convenient method to determine the collapse mechanisms of two types of dangerous rock masses (i.e. cantilever and upright) associated with the definition and calculation of the safety factor, which is based on the flexure theory of a constant-section beam by combining with the maximum tensile-stress criterion to depict the process of crack propagation caused by seismic waves. The calculation results show that there are critical crack depths in each form of the dangerous rock masses. Once the accumulated depth of the crack growth during an earthquake exceeds the critical depth, the collapse will occur. It is also demonstrated that the crack extension amount of each step is not a constant value, and is closely associated with the current accumulated crack depth. The greater the cumulative crack depth, the more easily the crack propagates. Finally, the validity and applicability of the proposed method are verified through two actual engineering examples.

  17. A Real Two-Phase Mechanical Model for Rock-Ice Avalanches

    NASA Astrophysics Data System (ADS)

    Pudasaini, S. P.; Krautblatter, M.

    2012-04-01

    Rock-ice avalanches in high mountain permafrost environments are a hazardous and poorly understood process. Their hazard potential derives from the large volume, high velocities, the potential entrainment of large amounts of rock-debris, ice, snow and water during the flow, high impact pressures, and unpredictable flow paths and deposition patterns. In contrast to the usual single-phase model of rock avalanches, the solid phase (ice) in rock-ice avalanches can transform to fluid (water or slurry) during the course of the debris-avalanche and fundamentally alter the multiple mechanical processes. We postulate that a real two-phase debris flow model could much better address the dynamic interaction of solid (rock and ice) and fluid (water, snow, slurry and fine particles) rather than a simple single-phase Voellmy- or Coulomb-type model. For this, we enhance the general two-phase debris flow model proposed by Pudasaini (2011) by additionally introducing two new mechanical aspects typical for the rock-ice avalanches: (a) the dynamic strength weakening including the internal fluidization and basal lubrication, as well as (b) the internal mass and momentum exchanges between the phases. In these models, the effective basal and internal friction angles are variable and are described in terms of evolving effective solid volume fraction (rock and ice), friction factors, volume fraction of the ice, true friction coefficients and the lubrication and fluidization factors. These factors are functions of several physical parameters and mechanical and dynamical variables, including the volume fractions of the solid, shear-rate and the normal stresses. Rock-ice avalanches are a unique scenario in geophysical mass flows, where phase exchange and material strength weakening occurs and can dominate the flow dynamics. Here, we present an innovative approach to model and simulate these two special aspects. Additionally, in the model, the inertial terms include the hydraulic pressure

  18. Influence of Water Content on Mechanical Properties of Rock in Both Saturation and Drying Processes

    NASA Astrophysics Data System (ADS)

    Zhou, Zilong; Cai, Xin; Cao, Wenzhuo; Li, Xibing; Xiong, Cheng

    2016-08-01

    Water content has a pronounced influence on the properties of rock materials, which is responsible for many rock engineering hazards, such as landslides and karst collapse. Meanwhile, water injection is also used for the prevention of some engineering disasters like rock-bursts. To comprehensively investigate the effect of water content on mechanical properties of rocks, laboratory tests were carried out on sandstone specimens with different water contents in both saturation and drying processes. The Nuclear Magnetic Resonance technique was applied to study the water distribution in specimens with variation of water contents. The servo-controlled rock mechanics testing machine and Split Hopkinson Pressure Bar technique were used to conduct both compressive and tensile tests on sandstone specimens with different water contents. From the laboratory tests, reductions of the compressive and tensile strength of sandstone under static and dynamic states in different saturation processes were observed. In the drying process, all of the saturated specimens could basically regain their mechanical properties and recover its strength as in the dry state. However, for partially saturated specimens in the saturation and drying processes, the tensile strength of specimens with the same water content was different, which could be related to different water distributions in specimens.

  19. Design, synthesis, and biological evaluation of novel, highly active soft ROCK inhibitors.

    PubMed

    Boland, Sandro; Bourin, Arnaud; Alen, Jo; Geraets, Jacques; Schroeders, Pieter; Castermans, Karolien; Kindt, Nele; Boumans, Nicki; Panitti, Laura; Fransen, Silke; Vanormelingen, Jessica; Stassen, Jean Marie; Leysen, Dirk; Defert, Olivier

    2015-05-28

    ROCK1 and ROCK2 play important roles in numerous cellular functions, including smooth muscle cell contraction, cell proliferation, adhesion, and migration. Consequently, ROCK inhibitors are of interest for treating multiple indications including cardiovascular diseases, inflammatory and autoimmune diseases, lung diseases, and eye diseases. However, systemic inhibition of ROCK is expected to result in significant side effects. Strategies allowing reduced systemic exposure are therefore of interest. In a continuing effort toward identification of ROCK inhibitors, we here report the design, synthesis, and evaluation of novel soft ROCK inhibitors displaying an ester function allowing their rapid inactivation in the systemic circulation. Those compounds display subnanomolar activity against ROCK and strong differences of functional activity between parent compounds and expected metabolites. The binding mode of a representative compound was determined experimentally in a single-crystal X-ray diffraction study. Enzymes responsible for inactivation of these compounds once they enter systemic circulation are also discussed.

  20. ROCK activity affects IL-1-induced signaling possibly through MKK4 and p38 MAPK in Caco-2 cells.

    PubMed

    Banerjee, Sayantan; McGee, Dennis W

    2016-09-01

    Elevated levels of interleukin-1 (IL-1) accompany inflammatory bowel disease. IL-1-stimulated intestinal epithelial cells can secrete potent chemokines like CXCL8 to exacerbate inflammation. Previously, we found that inhibiting the Rho-associated kinase (ROCK) could inhibit IL-1- or TNF-α-induced CXCL8 secretion by the Caco-2 colonic epithelial cell line. This ROCK inhibition did not affect IκBα phosphorylation and degradation, but suppressed the phosphorylation of c-Jun N-terminal kinase (JNK). Therefore, ROCK must play an important role in epithelial cell CXCL8 responses through an effect on the JNK signaling pathway. Here, we extend these studies by showing that inhibiting ROCK suppressed the IL-1-induced phosphorylation of MKK4, a known activator of JNK, but not MKK7. Yet, ROCK inhibition had no significant effect on the IL-1-induced phosphorylation of extracellular-signal-regulated kinase (ERK) 1/2. Inhibiting ROCK also suppressed the phosphorylation of p38 MAPK after IL-1 stimulation, but this inhibition had no significant effect on the stability of CXCL8 messenger RNA (mRNA) after IL-1 stimulation. These results suggest that ROCK may be important in IL-1-induced signaling through MKK4 to JNK and the activation of p38 MAPK. Finally, inhibiting ROCK in IL-1 and TNF-α co-stimulated Caco-2 cells also resulted in a significant suppression of CXCL8 secretion and mRNA levels suggesting that inhibiting ROCK may be a mechanism to inhibit the overall response of epithelial cells to both cytokines. These studies indicate a novel signaling event, which could provide a target for suppressing intestinal epithelial cells (IEC) chemokine responses involved in mucosal inflammation.

  1. ROCK activity affects IL-1-induced signaling possibly through MKK4 and p38 MAPK in Caco-2 cells.

    PubMed

    Banerjee, Sayantan; McGee, Dennis W

    2016-09-01

    Elevated levels of interleukin-1 (IL-1) accompany inflammatory bowel disease. IL-1-stimulated intestinal epithelial cells can secrete potent chemokines like CXCL8 to exacerbate inflammation. Previously, we found that inhibiting the Rho-associated kinase (ROCK) could inhibit IL-1- or TNF-α-induced CXCL8 secretion by the Caco-2 colonic epithelial cell line. This ROCK inhibition did not affect IκBα phosphorylation and degradation, but suppressed the phosphorylation of c-Jun N-terminal kinase (JNK). Therefore, ROCK must play an important role in epithelial cell CXCL8 responses through an effect on the JNK signaling pathway. Here, we extend these studies by showing that inhibiting ROCK suppressed the IL-1-induced phosphorylation of MKK4, a known activator of JNK, but not MKK7. Yet, ROCK inhibition had no significant effect on the IL-1-induced phosphorylation of extracellular-signal-regulated kinase (ERK) 1/2. Inhibiting ROCK also suppressed the phosphorylation of p38 MAPK after IL-1 stimulation, but this inhibition had no significant effect on the stability of CXCL8 messenger RNA (mRNA) after IL-1 stimulation. These results suggest that ROCK may be important in IL-1-induced signaling through MKK4 to JNK and the activation of p38 MAPK. Finally, inhibiting ROCK in IL-1 and TNF-α co-stimulated Caco-2 cells also resulted in a significant suppression of CXCL8 secretion and mRNA levels suggesting that inhibiting ROCK may be a mechanism to inhibit the overall response of epithelial cells to both cytokines. These studies indicate a novel signaling event, which could provide a target for suppressing intestinal epithelial cells (IEC) chemokine responses involved in mucosal inflammation. PMID:27173611

  2. A probabilistic approach to rock mechanical property characterization for nuclear waste repository design

    SciTech Connect

    Kim, Kunsoo; Gao, Hang

    1996-04-01

    A probabilistic approach is proposed for the characterization of host rock mechanical properties at the Yucca Mountain site. This approach helps define the probability distribution of rock properties by utilizing extreme value statistics and Monte Carlo simulation. We analyze mechanical property data of tuff obtained by the NNWSI Project to assess the utility of the methodology. The analysis indicates that laboratory measured strength and deformation data of Calico Hills and Bullfrog tuffs follow an extremal. probability distribution (the third type asymptotic distribution of the smallest values). Monte Carlo simulation is carried out to estimate rock mass deformation moduli using a one-dimensional tuff model proposed by Zimmermann and Finley. We suggest that the results of these analyses be incorporated into the repository design.

  3. Experimental Investigation of the Mechanical Behavior of Bedded Rocks and Its Implication for High Sidewall Caverns

    NASA Astrophysics Data System (ADS)

    Zhou, Yang-Yi; Feng, Xia-Ting; Xu, Ding-Ping; Fan, Qi-Xiang

    2016-09-01

    The stability of high sidewalls of large-span underground powerhouses will be a major issue when the cavern axis forms a small angle to the steeply inclined rock strata. A synthetic test scheme composed of four experiments was performed on two rocks with clear bedding features, aiming at better understanding the otherwise confusing deformation behavior and failure patterns of bedded rocks. Bedding orientations with respect to stress direction impose significant effect on the mechanical behavior of bedded rocks. Excessive tensile strain is observed in the direction perpendicular to bedding or across material interface in uniaxial test. Under low confinement in true triaxial test, the σ 2 angle mainly influences the deformation and fracture propagation but not strength. Deformation dependence of bedded rocks on two stress paths is thoroughly investigated. Confining pressure unloading leads to pronounced volumetric dilation accompanied by moduli drop. Samples with large bedding angle exhibit more obvious lateral dilation. Post-peak degradation of deformation parameters is confirmed by cyclic test. Fractures entirely or partly along bedding occurred under different stress states depend not only on the bonding strength between beds but on the anisotropic deformation field. Based on these observations, it is deduced that the possible reasons for the failure of steeply dipping rock mass after excavation are a combination of (1) the pervasive bedding planes, (2) the more pronounced deformation normal to bedding, and (3) the excavation-induced unloading of confinement.

  4. Effect of Particle Shape on Mechanical Behaviors of Rocks: A Numerical Study Using Clumped Particle Model

    PubMed Central

    Rong, Guan; Liu, Guang; Zhou, Chuang-bing

    2013-01-01

    Since rocks are aggregates of mineral particles, the effect of mineral microstructure on macroscopic mechanical behaviors of rocks is inneglectable. Rock samples of four different particle shapes are established in this study based on clumped particle model, and a sphericity index is used to quantify particle shape. Model parameters for simulation in PFC are obtained by triaxial compression test of quartz sandstone, and simulation of triaxial compression test is then conducted on four rock samples with different particle shapes. It is seen from the results that stress thresholds of rock samples such as crack initiation stress, crack damage stress, and peak stress decrease with the increasing of the sphericity index. The increase of sphericity leads to a drop of elastic modulus and a rise in Poisson ratio, while the decreasing sphericity usually results in the increase of cohesion and internal friction angle. Based on volume change of rock samples during simulation of triaxial compression test, variation of dilation angle with plastic strain is also studied. PMID:23997677

  5. Subcritical crack growth and mechanical weathering: a new consideration of how moisture influences rock erosion rates.

    NASA Astrophysics Data System (ADS)

    Eppes, Martha-Cary; Keanini, Russell; Hancock, Gregory S.

    2016-04-01

    The contributions of moisture to the mechanical aspects of rock weathering and regolith production are poorly quantified. In particular, geomorphologists have largely overlooked the role of subcritical crack growth processes in physical weathering and the fact that moisture strongly influences the rates of those processes. This influence is irrespective of the function that moisture plays in stress loading mechanisms like freezing or hydration. Here we present a simple numerical model that explores the efficacy of subcritical crack growth in granite rock subaerially exposed under a range of moisture conditions. Because most weathering-related stress loading for rocks found at, or near, Earth's surface (hereafter surface rocks) is cyclic, we modeled crack growth using a novel combination of Paris' Law and Charles' Law. This combination allowed us to apply existing empirically-derived data for the stress corrosion index of Charles' Law to fatigue cracking. For stress, we focused on the relatively straightforward case of intergranular stresses that arise during solar-induced thermal cycling by conductive heat transfer, making the assumption that such stresses represent a universal minimum weathering stress experienced by all surface rocks. Because all other tensile weathering-related stresses would be additive in the context of crack growth, however, our model can be adapted to include other stress loading mechanisms. We validated our calculations using recently published thermal-stress-induced cracking rates. Our results demonstrate that 1) weathering-induced stresses as modeled herein, and as published by others, are sufficient to propagate fractures subcritically over long timescales with or without the presence of water 2) fracture propagation rates increase exponentially with respect to moisture, specifically relative humidity 3) fracture propagation rates driven by thermal cycling are strongly dependent on the magnitude of diurnal temperature ranges and the

  6. Mechanical defradation of Emplacement Drifts at Yucca Mountain- A Modeling Case Study. Part I: Nonlithophysal Rock

    SciTech Connect

    M. Lin; D. Kicker; B. Damjanac; M. Board; M. Karakouzian

    2006-07-05

    This paper outlines rock mechanics investigations associated with mechanical degradation of planned emplacement drifts at Yucca Mountain, which is the designated site for the proposed U.S. high-level nuclear waste repository. The factors leading to drift degradation include stresses from the overburden, stresses induced by the heat released from the emplaced waste, stresses due to seismically related ground motions, and time-dependent strength degradation. The welded tuff emplacement horizon consists of two groups of rock with distinct engineering properties: nonlithophysal units and lithophysal units, based on the relative proportion of lithophysal cavities. The term 'lithophysal' refers to hollow, bubble like cavities in volcanic rock that are surrounded by a porous rim formed by fine-grained alkali feldspar, quartz, and other minerals. Lithophysae are typically a few centimeters to a few decimeters in diameter. Part I of the paper concentrates on the generally hard, strong, and fractured nonlithophysal rock. The degradation behavior of the tunnels in the nonlithophysal rock is controlled by the occurrence of keyblocks. A statistically equivalent fracture model was generated based on extensive underground fracture mapping data from the Exploratory Studies Facility at Yucca Mountain. Three-dimensional distinct block analyses, generated with the fracture patterns randomly selected from the fracture model, were developed with the consideration of in situ, thermal, and seismic loads. In this study, field data, laboratory data, and numerical analyses are well integrated to provide a solution for the unique problem of modeling drift degradation.

  7. Albite [yields] jadeite + quartz transformation in rock: Mechanism and kinetics

    SciTech Connect

    Bohlen, S.R.; Kirby, S.H. ); Hacker, B.R.

    1992-01-01

    Recent work on the calcite [yields] aragonite transformation using fully dense marble revealed significant differences from earlier experiments on powders and single-crystals. The reaction rate is retarded by a factor of > 1,000 and reaction mechanisms and resultant textures are considerably more complex. Stimulated by this, the authors conducted a study of the albite [yields] jadeite + quartz/coesite transformation in a fully dense albitite. Again the results are in marked contrast with previous powder-based studies of this archetypal metamorphic reaction. Solid cores of albitite were held at temperatures of 500-1,200 C and at pressure oversteps of 500 MPa into the jadeite + quartz stability field for 1--8 days in piston-cylinder apparatus. Samples that were dried in vacuum transformed appreciably only at temperatures in excess of 1,000 C. At all grain boundaries there is subequal transformation to micron-scale intergrowths of jadeite + quartz. Samples that were vacuum-impregnated with 1 wt% water contain jadeite + quartz to temperatures as low as 600 C. In contrast to the dried samples, transformation is much less homogeneous. The jadeite + quartz intergrowths do not form rows of subparallel crystals on grain boundaries, but rather are flower-shaped clusters that radiate outward from single nucleation sites at 3-grain edges and 4-grain corners. Compared to powders, pressure oversteps a factor of 10 greater are necessary to induce equivalent reaction in albitite. The sluggishness of this reaction has important implications for the evolution of the lower continental crust and subducting oceanic crust in terms of their (1) seismic velocity profiles, (2) petrological evolution, and (3) buoyancy forces, stresses and vertical crustal movements connected with densification and dilatational reactions.

  8. Thermo-mechanical pressurization of experimental faults in cohesive rocks during seismic slip

    NASA Astrophysics Data System (ADS)

    Violay, M.; Di Toro, G.; Nielsen, S.; Spagnuolo, E.; Burg, J. P.

    2015-11-01

    Earthquakes occur because fault friction weakens with increasing slip and slip rates. Since the slipping zones of faults are often fluid-saturated, thermo-mechanical pressurization of pore fluids has been invoked as a mechanism responsible for frictional dynamic weakening, but experimental evidence is lacking. We performed friction experiments (normal stress 25 MPa, maximal slip-rate ∼3 ms-1) on cohesive basalt and marble under (1) room-humidity and (2) immersed in liquid water (drained and undrained) conditions. In both rock types and independently of the presence of fluids, up to 80% of frictional weakening was measured in the first 5 cm of slip. Modest pressurization-related weakening appears only at later stages of slip. Thermo-mechanical pressurization weakening of cohesive rocks can be negligible during earthquakes due to the triggering of more efficient fault lubrication mechanisms (flash heating, frictional melting, etc.).

  9. REDBACK: an Open-Source Highly Scalable Simulation Tool for Rock Mechanics with Dissipative Feedbacks

    NASA Astrophysics Data System (ADS)

    Poulet, T.; Veveakis, M.; Paesold, M.; Regenauer-Lieb, K.

    2014-12-01

    Multiphysics modelling has become an indispensable tool for geoscientists to simulate the complex behaviours observed in their various fields of study where multiple processes are involved, including thermal, hydraulic, mechanical and chemical (THMC) laws. This modelling activity involves simulations that are computationally expensive and its soaring uptake is tightly linked to the increasing availability of supercomputing power and easy access to powerful nonlinear solvers such as PETSc (http://www.mcs.anl.gov/petsc/). The Multiphysics Object-Oriented Simulation Environment (MOOSE) is a finite-element, multiphysics framework (http://mooseframework.org) that can harness such computational power and allow scientists to develop easily some tightly-coupled fully implicit multiphysics simulations that run automatically in parallel on large clusters. This open-source framework provides a powerful tool to collaborate on numerical modelling activities and we are contributing to its development with REDBACK (https://github.com/pou036/redback), a module for Rock mEchanics with Dissipative feedBACKs. REDBACK builds on the tensor mechanics finite strain implementation available in MOOSE to provide a THMC simulator where the energetic formulation highlights the importance of all dissipative terms in the coupled system of equations. We show first applications of fully coupled dehydration reactions triggering episodic fluid transfer through shear zones (Alevizos et al, 2014). The dimensionless approach used allows focusing on the critical underlying variables which are driving the resulting behaviours observed and this tool is specifically designed to study material instabilities underpinning geological features like faulting, folding, boudinage, shearing, fracturing, etc. REDBACK provides a collaborative and educational tool which captures the physical and mathematical understanding of such material instabilities and provides an easy way to apply this knowledge to realistic

  10. Mechanically Active Electrospun Materials

    NASA Astrophysics Data System (ADS)

    Robertson, Jaimee M.

    Electrospinning, a technique used to fabricate small diameter polymer fibers, has been employed to develop unique, active materials falling under two categories: (1) shape memory elastomeric composites (SMECs) and (2) water responsive fiber mats. (1) Previous work has characterized in detail the properties and behavior of traditional SMECs with isotropic fibers embedded in an elastomer matrix. The current work has two goals: (i) characterize laminated anisotropic SMECs and (ii) develop a fabrication process that is scalable for commercial SMEC manufacturing. The former ((i)) requires electrospinning aligned polymer fibers. The aligned fibers are similarly embedded in an elastomer matrix and stacked at various fiber orientations. The resulting laminated composite has a unique response to tensile deformation: after stretching and releasing, the composite curls. This curling response was characterized based on fiber orientation. The latter goal ((ii)) required use of a dual-electrospinning process to simultaneously electrospin two polymers. This fabrication approach incorporated only industrially relevant processing techniques, enabling the possibility of commercial application of a shape memory rubber. Furthermore, the approach had the added benefit of increased control over composition and material properties. (2) The strong elongational forces experienced by polymer chains during the electrospinning process induce molecular alignment along the length of electrospun fibers. Such orientation is maintained in the fibers as the polymer vitrifies. Consequently, residual stress is stored in electrospun fiber mats and can be recovered by heating through the polymer's glass transition temperature. Alternatively, the glass transition temperature can be depressed by introducing a plasticizing agent. Poly(vinyl acetate) (PVAc) is plasticized by water, and its glass transition temperature is lowered below room temperature. Therefore, the residual stress can be relaxed at room

  11. Quantifying the impact of lithology upon the mechanical properties of rock

    NASA Astrophysics Data System (ADS)

    Weatherley, Dion

    2013-04-01

    The physical characteristics of rock, its lithology, undoubtedly influences its deformation under natural or engineering loads. Mineral texture, micro-damage, joints, bedding planes, inclusions, unconformities and faults are all postulated to alter the mechanical response of rock on different scales and under different stressing conditions. Whilst laboratory studies have elucidated some aspects of the relationship between lithology and mechanical properties, these small-scale results are difficult to extrapolate to lithospheric scales. To augment laboratory-derived knowledge, physics-based numerical modelling is a promising avenue [3]. Bonded particle models implemented using the Discrete Element Method (DEM [1]) are a practical numerical laboratory to investigate the interplay between lithology and the mechanical response of rock specimens [4]. Numerical rock specimens are represented as an assembly of indivisible spherical particles connected to nearest neighbours via brittle-elastic beams which impart forces and moments upon one-another as particles move relative to each other. By applying boundary forces and solving Newton's Laws for each particle, elastic deformation and brittle failure may be simulated [2]. Each beam interaction is defined by four model parameters: Young's modulus, Poisson's ratio, cohesive strength and internal friction angle. Beam interactions in different subvolumes of the specimen are assigned different parameters to model different rock types or mineral assemblages. Micro-cracks, joints, unconformities and faults are geometrically incorporated by fitting particles to either side of triangulated surfaces [5]. The utility of this modelling approach is verified by reproducing analytical results from fracture mechanics (Griffith crack propagation and wing-crack formation) and results of controlled laboratory investigations. To quantify the impact of particular lithologic structures on mechanical response, a range of control experiments are

  12. Geostatistical Evaluation of the Mechanical Properties of Rock Mass for TBM Tunnelling by Seismic Reflection Method

    NASA Astrophysics Data System (ADS)

    Aoki, K.; Mito, Y.; Yamamoto, T.; Shirasagi, S.

    2007-12-01

    The evaluation of the rock mass mechanical properties by the seismic reflection method and TBM driving is proposed for TBM tunnelling. The relationship between the reflection number derived from the three-dimensional seismic reflection method and the rock strength index ( RSI) derived from TBM driving data is examined, and the methodology of conversion from the reflection number to the RSI is proposed. Furthermore a geostatistical prediction methodology to provide a three-dimensional geotechnical profile ahead of the tunnel face is proposed. The performance of this prediction method is verified by actual field data.

  13. Hydromechanical coupling in fractured rock masses: mechanisms and processes of selected case studies

    NASA Astrophysics Data System (ADS)

    Zangerl, Christian

    2015-04-01

    in the range of millimetres to a very few centimetres and can be linked to annual groundwater fluctuations. Due to pore pressure variations HM coupling can influence seepage forces and effective stresses in the rock mass. Effective stress changes can adversely affect the stability and deformation behaviour of deep-seated rock slides by influencing the shear strength or the time dependent (viscous) material behaviour of the basal shear zone. The shear strength of active shear zones is often reasonably well described by Coulomb's law. In Coulomb's law the operative normal stresses to the shear surface/zone are effective stresses and hence pore pressures which should be taken into account reduces the shear strength. According to the time dependent material behaviour a few effective stress based viscous models exists which are able to consider pore pressures. For slowly moving rock slides HM coupling could be highly relevant when low-permeability clayey-silty shear zones (fault gouges) are existing. An important parameters therefore is the hydraulic diffusivity, which is controlled by the permeability and fluid-pore compressibility of the shear zone, and by fluid viscosity. Thus time dependent pore pressure diffusion in the shear zone can either control the stability condition or the viscous behaviour (creep) of the rock slide. Numerous cases studies show that HM coupling can effect deformability, shear strength and time dependent behaviour of fractured rock masses. A process-based consideration can be important to avoid unexpected impacts on infrastructures and to understand complex rock mass as well rock slide behaviour.

  14. Proposed healing and consolidation mechanisms of rock salt revealed by ESEM.

    PubMed

    Hwang, C L; Wang, M L; Miao, S

    1993-08-01

    The grain boundary healing behavior of crushed rock salt was mainly studied by employing the environmental scanning electron microscope (ESEM) to study the consolidation mechanism of rock salt backfill. Dedicated miniature round rock salt specimens were prepared for observation of the water trapping effect by using a cold stage in the ESEM to reach saturation conditions. Comparable high pressure pellets were prepared for measuring the crystal growth. Consolidation tests using materials made at different pressures and containing different moisture levels were conducted in order to construct the proposed mechanism. Direct observation of specimens in the ESEM resulted in viewing water trapped on the surface and the formation of a water meniscus between two particles. The concentration of brine at the grain boundary was observed as contributing to the amount of recrystallization. From aforementioned observations, a schematic drawing of the dissolution and recrystallization process may be redrawn. The amount of water therefore has a great effect on the consolidation of rock salt and is possibly due to the sliding, rotation, or crushing of the contact zone of the granular material. From such a study, tentative healing and consolidation mechanisms can be deduced.

  15. Rock-Mechanics Research. A Survey of United States Research to 1965, with a Partial Survey of Canadian Universities.

    ERIC Educational Resources Information Center

    National Academy of Sciences - National Research Council, Washington, DC.

    The results of a survey, conducted by the Committee on Rock Mechanics, to determine the status of training and research in rock mechanics in presented in this publication. In 1964 and 1965 information was gathered by questionnaires sent to industries, selected federal agencies, and universities in both the United States and Canada. Results are…

  16. A two-phase mechanical model for rock-ice avalanches

    NASA Astrophysics Data System (ADS)

    Pudasaini, Shiva P.; Krautblatter, Michael

    2014-10-01

    Rock-ice avalanche events are among the most hazardous natural disasters in the last century. In contrast to rock avalanches, the solid phase (ice) can transform to fluid during the course of the rock-ice avalanche and fundamentally alter mechanical processes. A real two-phase debris flow model could better address the dynamic interaction of solid (rock and ice) and fluid (water, snow, slurry, and fine particles) than presently used single-phase Voellmy- or Coulomb-type models. We present a two-phase model capable of performing dynamic strength weakening due to internal fluidization and basal lubrication and internal mass and momentum exchanges between the phases. Effective basal and internal friction angles are variable and correspond to evolving effective solid volume fraction, friction factors, volume fraction of the ice, true friction coefficients, and lubrication and fluidization factors. Benchmark numerical simulations demonstrate that the two-phase model can explain dynamically changing frictional properties of rock-ice avalanches that occur internally and along the flow path. The interphase mass and momentum exchanges are capable of demonstrating the mechanics of frontal surge head and multiple other surges in the debris body. This is an observed phenomenon in a real two-phase debris flow, but newly simulated here by applying the two-phase mass flow model. Mass and momentum exchanges between the phases and the associated internal and basal strength weakening control the exceptional long runout distances, provide a more realistic simulation especially during the critical initial and propagation stages of avalanche, and explain the exceptionally high and dynamically changing mobility of rock-ice avalanches.

  17. Pitted rock surfaces on Mars: A mechanism of formation by transient melting of snow and ice

    NASA Astrophysics Data System (ADS)

    Head, James W.; Kreslavsky, Mikhail A.; Marchant, David R.

    2011-09-01

    Pits in rocks on the surface of Mars have been observed at several locations. Similar pits are observed in rocks in the Mars-like hyperarid, hypothermal stable upland zone of the Antarctic Dry Valleys; these form by very localized chemical weathering due to transient melting of small amounts of snow on dark dolerite boulders preferentially heated above the melting point of water by sunlight. We examine the conditions under which a similar process might explain the pitted rocks seen on the surface of Mars (rock surface temperatures above the melting point; atmospheric pressure exceeding the triple point pressure of H2O; an available source of solid water to melt). We find that on Mars today each of these conditions is met locally and regionally, but that they do not occur together in such a way as to meet the stringent requirements for this process to operate. In the geological past, however, conditions favoring this process are highly likely to have been met. For example, increases in atmospheric water vapor content (due, for example, to the loss of the south perennial polar CO2 cap) could favor the deposition of snow, which if collected on rocks heated to above the melting temperature during favorable conditions (e.g., perihelion), could cause melting and the type of locally enhanced chemical weathering that can cause pits. Even when these conditions are met, however, the variation in heating of different rock facets under Martian conditions means that different parts of the rock may weather at different times, consistent with the very low weathering rates observed on Mars. Furthermore, as is the case in the stable upland zone of the Antarctic Dry Valleys, pit formation by transient melting of small amounts of snow readily occurs in the absence of subsurface active layer cryoturbation.

  18. ROCK1 in AgRP neurons regulates energy expenditure and locomotor activity in male mice.

    PubMed

    Huang, Hu; Lee, Seung Hwan; Ye, Chianping; Lima, Ines S; Oh, Byung-Chul; Lowell, Bradford B; Zabolotny, Janice M; Kim, Young-Bum

    2013-10-01

    Normal leptin signaling is essential for the maintenance of body weight homeostasis. Proopiomelanocortin- and agouti-related peptide (AgRP)-producing neurons play critical roles in regulating energy metabolism. Our recent work demonstrates that deletion of Rho-kinase 1 (ROCK1) in the AgRP neurons of mice increased body weight and adiposity. Here, we report that selective loss of ROCK1 in AgRP neurons caused a significant decrease in energy expenditure and locomotor activity of mice. These effects were independent of any change in food intake. Furthermore, AgRP neuron-specific ROCK1-deficient mice displayed central leptin resistance, as evidenced by impaired Signal Transducer and Activator of Transcription 3 activation in response to leptin administration. Leptin's ability to hyperpolarize and decrease firing rate of AgRP neurons was also abolished in the absence of ROCK1. Moreover, diet-induced and genetic forms of obesity resulted in reduced ROCK1 activity in murine arcuate nucleus. Of note, high-fat diet also impaired leptin-stimulated ROCK1 activity in arcuate nucleus, suggesting that a defect in hypothalamic ROCK1 activity may contribute to the pathogenesis of central leptin resistance in obesity. Together, these data demonstrate that ROCK1 activation in hypothalamic AgRP neurons is required for the homeostatic regulation of energy expenditure and adiposity. These results further support previous work identifying ROCK1 as a key regulator of energy balance and suggest that targeting ROCK1 in the hypothalamus may lead to development of antiobesity therapeutics. PMID:23885017

  19. Analytical and Numerical Study of the Mechanics of Rockbolt Reinforcement around Tunnels in Rock Masses

    NASA Astrophysics Data System (ADS)

    Carranza-Torres, C.

    2009-04-01

    This paper addresses the problem of quantifying the mechanical contribution of rockbolts installed systematically around tunnels excavated in rock masses. The mechanical contribution referred to here is that of increased stress confinement and decreased tunnel convergences as compared with corresponding stresses and displacements obtained for non-reinforced tunnels. The problem is treated analytically first by presenting a closed-form solution for stress and displacement distributions around a circular tunnel excavated in elastic material and reinforced by grouted or anchored rockbolts. The analytical solution assumes that rockbolts are regularly spaced around the tunnel and that axi-symmetry conditions of geometry and loading apply. The results obtained with the closed-form solution are shown to be equivalent to the results of the same problem solved with traditional numerical methods. Based on the analytical and numerical results and by introducing dimensionless ratios that allow to quantify the increase of radial stresses and the decrease of radial displacements in the reinforced region of the tunnel, the paper shows that reinforcement can have a significant mechanical effect (i.e., increasing the confinement and decreasing the convergences) in tunnels excavated in rock masses of poor to very poor quality. The paper analyzes then the mechanical contribution of rockbolt reinforcement when the rock mass is assumed to behave elasto-plastically. For this case, it is shown that rockbolt reinforcement can also have a critical effect in controlling the extent of the plastic failure zone and the convergences of the tunnel.

  20. Sensitivity Analysis of Mechanical Parameters of Different Rock Layers to the Stability of Coal Roadway in Soft Rock Strata

    PubMed Central

    Zhao, Zeng-hui; Wang, Wei-ming; Gao, Xin; Yan, Ji-xing

    2013-01-01

    According to the geological characteristics of Xinjiang Ili mine in western area of China, a physical model of interstratified strata composed of soft rock and hard coal seam was established. Selecting the tunnel position, deformation modulus, and strength parameters of each layer as influencing factors, the sensitivity coefficient of roadway deformation to each parameter was firstly analyzed based on a Mohr-Columb strain softening model and nonlinear elastic-plastic finite element analysis. Then the effect laws of influencing factors which showed high sensitivity were further discussed. Finally, a regression model for the relationship between roadway displacements and multifactors was obtained by equivalent linear regression under multiple factors. The results show that the roadway deformation is highly sensitive to the depth of coal seam under the floor which should be considered in the layout of coal roadway; deformation modulus and strength of coal seam and floor have a great influence on the global stability of tunnel; on the contrary, roadway deformation is not sensitive to the mechanical parameters of soft roof; roadway deformation under random combinations of multi-factors can be deduced by the regression model. These conclusions provide theoretical significance to the arrangement and stability maintenance of coal roadway. PMID:24459447

  1. Rock Mechanics and Enhanced Geothermal Systems: A DOE-sponsored Workshop to Explore Research Needs

    SciTech Connect

    Francois Heuze; Peter Smeallie; Derek Elsworth; Joel L. Renner

    2003-10-01

    This workshop on rock mechanics and enhanced geothermal systems (EGS) was held in Cambridge, Mass., on June 20-21 2003, before the Soil and Rock America 2003 International Conference at MIT. Its purpose was to bring together experts in the field of rock mechanics and geothermal systems to encourage innovative thinking, explore new ideas, and identify research needs in the areas of rock mechanics and rock engineering applied to enhanced geothermal systems. The agenda is shown in Appendix A. The workshop included experts in the fields of rock mechanics and engineering, geological engineering, geophysics, drilling, the geothermal energy production from industry, universities and government agencies, and laboratories. The list of participants is shown is Appendix B. The first day consisted of formal presentations. These are summarized in Chapter 1 of the report. By the end of the first day, two broad topic areas were defined: reservoir characterization and reservoir performance. Working groups were formed for each topic. They met and reported in plenary on the second day. The working group summaries are described in Chapter 2. The final session of the workshop was devoted to reaching consensus recommendations. These recommendations are given in Chapter 3. That objective was achieved. All the working group recommendations were considered and, in order to arrive at a practical research agenda usable by the workshop sponsors, workshop recommendations were reduced to a total of seven topics. These topics were divided in three priority groups, as follows. First-priority research topics (2): {sm_bullet} Define the pre-existing and time-dependent geometry and physical characteristics of the reservoir and its fracture network. That includes the identification of hydraulically controlling fractures. {sm_bullet} Characterize the physical and chemical processes affecting the reservoir geophysical parameters and influencing the transport properties of fractures. Incorporate those

  2. Expectations of Rock Music Consumption for Entertainment and Information Relative to the Active Involvement of the User.

    ERIC Educational Resources Information Center

    Rouner, Donna; Noyes, Amy

    Before examining potentially negative effects of rock music on adolescents, it is necessary to demonstrate links between adolescent motivations for consuming rock music and active involvement relative to that use and also to consider how much rock listeners rely on rock music as a source for information about values, beliefs, and social…

  3. Whole-rock uranium analysis by fission track activation

    NASA Technical Reports Server (NTRS)

    Weiss, J. R.; Haines, E. L.

    1974-01-01

    We report a whole-rock uranium method in which the polished sample and track detector are separated in a vacuum chamber. Irradiation with thermal neutrons induces uranium fission in the sample, and the detector records the integrated fission track density. Detection efficiency and geometric factors are calculated and compared with calibration experiments.

  4. Active Auditory Mechanics in Insects

    NASA Astrophysics Data System (ADS)

    Robert, D.; Göpfert, M. C.

    2003-02-01

    Evidence is presented that hearing in some insects is an active process. Audition in mosquitoes is used for mate-detection and is supported by antennal receivers, whose sound-induced vibrations are transduced by Johnston's organs. Each of these sensory organs contains ca. 15,000 sensory neurons. As shown by mechanical analysis, a physiologically vulnerable mechanism is at work that nonlinearly enhances the sensitivity and frequency selectivity of antennal hearing. This process of amplification correlates with the electrical activity of the auditory mechanoreceptor units in Johnston's organ.

  5. ACOUSTICAL IMAGING AND MECHANICAL PROPERTIES OF SOFT ROCK AND MARINE SEDIMENTS

    SciTech Connect

    Thurman E. Scott, Jr., Ph.D.; Younane Abousleiman, Ph.D.; Musharraf Zaman, Ph.D., P.E.

    2001-07-01

    Mechanically weak formations, such as chalks, high porosity sandstones, and marine sediments, pose significant problems for oil and gas operators. Problems such as compaction, subsidence, and loss of permeability can affect reservoir production operations. For example, the unexpected subsidence of the Ekofisk chalk in the North Sea required over one billion dollars to re-engineer production facilities to account for losses created during that compaction (Sulak 1991). Another problem in weak formations is that of shallow water flows (SWF). Deep water drilling operations sometimes encounter cases where the marine sediments, at shallow depths just below the seafloor, begin to uncontrollably flow up and around the drill pipe. SWF problems created a loss of $150 million for the Ursa development project in the U.S. Gulf Coast SWF (Furlow 1998a,b; 1999a,b). The goal of this project is to provide a database on both the rock mechanical properties and the geophysical properties of weak rocks and sediments. These could be used by oil and gas companies to detect, evaluate, and alleviate potential production and drilling problems. The results will be useful in, for example, pre-drill detection of events such as SWF's by allowing a correlation of seismic data (such as hazard surveys) to rock mechanical properties. The data sets could also be useful for 4-D monitoring of the compaction and subsidence of an existing reservoir and imaging the zones of damage. During the second quarter of the project the research team has: (1) completed acoustic sensor construction, (2) conducted reconnaissance tests to map the deformational behaviors of the various rocks, (3) developed a sample assembly for the measurement of dynamic elastic and poroelastic parameters during triaxial testing, and (4) conducted a detailed review of the scientific literature and compiled a bibliography of that review. During the first quarter of the project the research team acquired several rock types for testing

  6. Mechanical properties of carboniferous rocks in the Upper Silesian Coal Basin under uniaxial and triaxial compression tests

    SciTech Connect

    Bukowska, M.

    2005-04-01

    Many years' studies of geological properties of rocks from the Upper Silesian Coal Basin have resulted in acquisition of a substantial data base of mechanical parameters of rocks over the total strain range. It is found that the post-peak rock properties are closely related with the peak strength and the pre-peak properties. The relationship between the uniaxial ultimate strength, elastic modulus, and drop modulus are determined.

  7. Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism.

    PubMed

    Riveline, D; Zamir, E; Balaban, N Q; Schwarz, U S; Ishizaki, T; Narumiya, S; Kam, Z; Geiger, B; Bershadsky, A D

    2001-06-11

    The transition of cell-matrix adhesions from the initial punctate focal complexes into the mature elongated form, known as focal contacts, requires GTPase Rho activity. In particular, activation of myosin II-driven contractility by a Rho target known as Rho-associated kinase (ROCK) was shown to be essential for focal contact formation. To dissect the mechanism of Rho-dependent induction of focal contacts and to elucidate the role of cell contractility, we applied mechanical force to vinculin-containing dot-like adhesions at the cell edge using a micropipette. Local centripetal pulling led to local assembly and elongation of these structures and to their development into streak-like focal contacts, as revealed by the dynamics of green fluorescent protein-tagged vinculin or paxillin and interference reflection microscopy. Inhibition of Rho activity by C3 transferase suppressed this force-induced focal contact formation. However, constitutively active mutants of another Rho target, the formin homology protein mDia1 (Watanabe, N., T. Kato, A. Fujita, T. Ishizaki, and S. Narumiya. 1999. Nat. Cell Biol. 1:136-143), were sufficient to restore force-induced focal contact formation in C3 transferase-treated cells. Force-induced formation of the focal contacts still occurred in cells subjected to myosin II and ROCK inhibition. Thus, as long as mDia1 is active, external tension force bypasses the requirement for ROCK-mediated myosin II contractility in the induction of focal contacts. Our experiments show that integrin-containing focal complexes behave as individual mechanosensors exhibiting directional assembly in response to local force. PMID:11402062

  8. The microstructural character and evolution of fault rocks from SAFOD and potential weakening mechanisms along the San Andreas Fault

    NASA Astrophysics Data System (ADS)

    van Diggelen, E.; Holdsworth, R. E.; de Bresser, J. H.; Spiers, C.; Smith, S. A.; Walker, R. J.; Bowen, L.

    2010-12-01

    The San Andreas Fault (SAF) forms the boundary between two geological terranes; the Salinian block (SB, Pacific plate) and the Great Valley block (GVB, North American plate). The SB contains arkosic sandstones, the GVB consists mostly of claystones and siltstones. The SAFOD borehole provides an extensive set of samples across the SAF and permits direct study of fault zone processes at 2-3 km depth. In order to determine the fault rock properties and deformation mechanisms in the SAF, in particular in two actively creeping fault segments, we have visually assessed the SAFOD phase 3 core material and we have performed detailed optical and electron microscopy, including chemical analyses using EDX. We compared the natural microstructures with microstructures developed in simulated fault gouges deformed in laboratory experiments. The rocks in Core interval 1 (SB) are mildly deformed and show evidence of cataclasis, pressure solution and reaction of feldspar to form phyllosilicates. Most of Core interval 3 (GVB) is also only mildly deformed, similar to Core interval 1. Sedimentary features are still visible, together with limited evidence for cataclasis, pressure solution and reaction of feldspar to phyllosilicates. The rocks in Core interval 2 (GVB) show ample evidence for micro-folding, foliation development, development of anastomosing shear bands, gouge formation, veining, and reworking of earlier microstructures. In addition, evidence is widespread for cataclasis, pressure solution and reaction of feldspar to form phyllosilicates. The SB and GVB host rocks are cut by numerous minor faults and small calcite-filled veins. Thin foliated gouges contain fine-grained, Fe-rich smectitic phyllosilicates. The development of interconnected networks of these phyllosilicates following cataclasis is prevalent in the inactive gouges. The actively creeping zones in Core intervals 2 and 3 consist mostly of Mg-rich smectitic phyllosilicates and show a strong, wavy foliation, lens

  9. Analysis on the Rock-Cutter Interaction Mechanism During the TBM Tunneling Process

    NASA Astrophysics Data System (ADS)

    Yang, Haiqing; Wang, He; Zhou, Xiaoping

    2016-03-01

    The accurate prediction of rock cutting forces of disc cutters is crucial for tunnel boring machine (TBM) design and construction. Disc cutter wear, which affects TBM penetration performance, has frequently been found at TBM sites. By considering the operating path and wear of the disc cutter, a new model is proposed for evaluating the cutting force and wear of the disc cutter in the tunneling process. The circular path adopted herein, which is the actual running path of the TBM disc cutter, shows that the lateral force of the disc cutter is asymmetric. The lateral forces on the sides of the disc cutter are clearly different. However, traditional solutions are obtained by assuming a linear path, where the later forces are viewed as equal. To simulate the interaction between the rock and disc cutter, a simple brittle damage model for rock mass is introduced here. Based on the explicit dynamic finite element method, the cutting force acting on the rock generated by a single disc cutter is simulated. It is shown that the lateral cutting force of the disc cutter strongly affects the wear extent of disc cutter. The wear mechanism is thus underestimated by the classical model, which was obtained by linear cutting tests. The simulation results are discussed and compared with other models, and these simulation results agree well with the results of present ones.

  10. A Negative Regulatory Mechanism Involving 14-3-3ζ Limits Signaling Downstream of ROCK to Regulate Tissue Stiffness in Epidermal Homeostasis.

    PubMed

    Kular, Jasreen; Scheer, Kaitlin G; Pyne, Natasha T; Allam, Amr H; Pollard, Anthony N; Magenau, Astrid; Wright, Rebecca L; Kolesnikoff, Natasha; Moretti, Paul A; Wullkopf, Lena; Stomski, Frank C; Cowin, Allison J; Woodcock, Joanna M; Grimbaldeston, Michele A; Pitson, Stuart M; Timpson, Paul; Ramshaw, Hayley S; Lopez, Angel F; Samuel, Michael S

    2015-12-21

    ROCK signaling causes epidermal hyper-proliferation by increasing ECM production, elevating dermal stiffness, and enhancing Fak-mediated mechano-transduction signaling. Elevated dermal stiffness in turn causes ROCK activation, establishing mechano-reciprocity, a positive feedback loop that can promote tumors. We have identified a negative feedback mechanism that limits excessive ROCK signaling during wound healing and is lost in squamous cell carcinomas (SCCs). Signal flux through ROCK was selectively tuned down by increased levels of 14-3-3ζ, which interacted with Mypt1, a ROCK signaling antagonist. In 14-3-3ζ(-/-) mice, unrestrained ROCK signaling at wound margins elevated ECM production and reduced ECM remodeling, increasing dermal stiffness and causing rapid wound healing. Conversely, 14-3-3ζ deficiency enhanced cutaneous SCC size. Significantly, inhibiting 14-3-3ζ with a novel pharmacological agent accelerated wound healing 2-fold. Patient samples of chronic non-healing wounds overexpressed 14-3-3ζ, while cutaneous SCCs had reduced 14-3-3ζ. These results reveal a novel 14-3-3ζ-dependent mechanism that negatively regulates mechano-reciprocity, suggesting new therapeutic opportunities.

  11. A Negative Regulatory Mechanism Involving 14-3-3ζ Limits Signaling Downstream of ROCK to Regulate Tissue Stiffness in Epidermal Homeostasis.

    PubMed

    Kular, Jasreen; Scheer, Kaitlin G; Pyne, Natasha T; Allam, Amr H; Pollard, Anthony N; Magenau, Astrid; Wright, Rebecca L; Kolesnikoff, Natasha; Moretti, Paul A; Wullkopf, Lena; Stomski, Frank C; Cowin, Allison J; Woodcock, Joanna M; Grimbaldeston, Michele A; Pitson, Stuart M; Timpson, Paul; Ramshaw, Hayley S; Lopez, Angel F; Samuel, Michael S

    2015-12-21

    ROCK signaling causes epidermal hyper-proliferation by increasing ECM production, elevating dermal stiffness, and enhancing Fak-mediated mechano-transduction signaling. Elevated dermal stiffness in turn causes ROCK activation, establishing mechano-reciprocity, a positive feedback loop that can promote tumors. We have identified a negative feedback mechanism that limits excessive ROCK signaling during wound healing and is lost in squamous cell carcinomas (SCCs). Signal flux through ROCK was selectively tuned down by increased levels of 14-3-3ζ, which interacted with Mypt1, a ROCK signaling antagonist. In 14-3-3ζ(-/-) mice, unrestrained ROCK signaling at wound margins elevated ECM production and reduced ECM remodeling, increasing dermal stiffness and causing rapid wound healing. Conversely, 14-3-3ζ deficiency enhanced cutaneous SCC size. Significantly, inhibiting 14-3-3ζ with a novel pharmacological agent accelerated wound healing 2-fold. Patient samples of chronic non-healing wounds overexpressed 14-3-3ζ, while cutaneous SCCs had reduced 14-3-3ζ. These results reveal a novel 14-3-3ζ-dependent mechanism that negatively regulates mechano-reciprocity, suggesting new therapeutic opportunities. PMID:26702834

  12. Hitherto unknown shear rupture mechanism as a source of instability in intact hard rocks at highly confined compression

    NASA Astrophysics Data System (ADS)

    Tarasov, Boris G.

    2014-05-01

    Today, frictional shear resistance along pre-existing faults is considered to be the lower limit on rock shear strength for confined conditions corresponding to the seismogenic layer. This paper introduces a recently identified shear rupture mechanism providing a paradoxical feature of hard rocks - the possibility of shear rupture propagation through the highly confined intact rock mass at shear stress levels significantly less than frictional strength. In the new mechanism, the rock failure associated with consecutive creation of small slabs (known as ‘domino-blocks') from the intact rock in the rupture tip is driven by a fan-shaped domino structure representing the rupture head. The fan-head combines such unique features as: extremely low shear resistance, self-sustaining stress intensification, and self-unbalancing conditions. Due to this the failure process caused by the mechanism is very dynamic and violent. This makes it impossible to directly observe and study the mechanism and can explain why the mechanism has not been detected before. This paper provides physical motivation for the mechanism, based upon side effects accompanying the failure process. Physical and mathematical models of the mechanism presented in the paper explain unique and paradoxical features of the mechanism. The new shear rupture mechanism allows a novel point of view for understanding the nature of spontaneous failure processes in hard rocks including earthquakes.

  13. ACOUSTICAL IMAGING AND MECHANICAL PROPERTIES OF SOFT ROCK AND MARINE SEDIMENTS

    SciTech Connect

    Thurman E. Scott, Jr., Ph.D.; Younane Abousleiman, Ph.D.; Musharraf Zaman, Ph.D., P.E.

    2002-11-18

    During the seven quarter of the project the research team analyzed some of the acoustic velocity data and rock deformation data. The goal is to create a series of ''deformation-velocity maps'' which can outline the types of rock deformational mechanisms which can occur at high pressures and then associate those with specific compressional or shear wave velocity signatures. During this quarter, we began to analyze both the acoustical and deformational properties of the various rock types. Some of the preliminary velocity data from the Danian chalk will be presented in this report. This rock type was selected for the initial efforts as it will be used in the tomographic imaging study outlined in Task 10. This is one of the more important rock types in the study as the Danian chalk is thought to represent an excellent analog to the Ekofisk chalk that has caused so many problems in the North Sea. Some of the preliminary acoustic velocity data obtained during this phase of the project indicates that during pore collapse and compaction of this chalk, the acoustic velocities can change by as much as 200 m/s. Theoretically, this significant velocity change should be detectable during repeated successive 3-D seismic images. In addition, research continues with an analysis of the unconsolidated sand samples at high confining pressures obtained in Task 9. The analysis of the results indicate that sands with 10% volume of fines can undergo liquefaction at lower stress conditions than sand samples which do not have fines added. This liquefaction and/or sand flow is similar to ''shallow water'' flows observed during drilling in the offshore Gulf of Mexico.

  14. A brief guide to synchrotron radiation-based microtomography in (structural) geology and rock mechanics

    NASA Astrophysics Data System (ADS)

    Fusseis, F.; Xiao, X.; Schrank, C.; De Carlo, F.

    2014-08-01

    This contribution outlines Synchrotron-based X-ray micro-tomography and its potential use in structural geology and rock mechanics. The paper complements several recent reviews of X-ray microtomography. We summarize the general approach to data acquisition, post-processing as well as analysis and thereby aim to provide an entry point for the interested reader. The paper includes tables listing relevant beamlines, a list of all available imaging techniques, and available free and commercial software packages for data visualization and quantification. We highlight potential applications in a review of relevant literature including time-resolved experiments and digital rock physics. The paper concludes with a report on ongoing developments and upgrades at synchrotron facilities to frame the future possibilities for imaging sub-second processes in centimetre-sized samples.

  15. Determination of basic physical and mechanical properties of basaltic rocks from P-wave velocity

    NASA Astrophysics Data System (ADS)

    Karakuş, Askeri; Akatay, Mahmut

    2013-12-01

    Physical and mechanical properties of basaltic rocks used as main building material in historical buildings in Diyarbakir show great diversity depending on the place of origin. Especially, earthquake studies as well as restoration jobs and civil engineers and architects who work on building dynamics need to know basic material properties of basaltic rocks that are the main building material. In this study, the basalt samples obtained from 18 different locations of the Diyarbakir area were tested in order to estimate the main material properties of basalts used in historical buildings without collecting samples from them. Subsequently, statistical relationships between the nondestructive P-wave velocity and other properties of basalts were investigated. Consequently, highly correlated models (R2 = 0.717-0.890) were obtained between P-wave velocity and density, porosity, uniaxial compressive strength, Brazilian tensile strength, modulus of elasticity and Poisson's ratio.

  16. Estimating changes in rock permeability due to thermal-mechanical effects

    SciTech Connect

    Wang, H.F.; Blair, S.C.; Berge, P.A.

    1997-10-01

    This paper presents results of a modeling study of changes in fracture permeability due to thermal-mechanical effects associated with the potential geological repository at Yucca Mountain. a methodology for estimating changes in permeability is developed and applied to the Drift Scale Test (DST) now being conducted in the Exploratory Studies Facility (ESF) at Yucca Mountain. Temperature, stress, and displacement of rock in the heated zone are presented along with predicted zones where slip on fractures may occur. The zones of predicted fracture slip are used as a basis for predicting where permeability may be changed. this new procedure goes beyond previous models that relate stress to strain or displacement, and provides information about rock response that is needed for design of future tests at Yucca Mountain. Our results also contribute to the understanding of coupled processes in the near-field environment of a repository.

  17. Low-Dose Endothelial Monocyte-Activating Polypeptide-II Increases Blood-Tumor Barrier Permeability by Activating the RhoA/ROCK/PI3K Signaling Pathway.

    PubMed

    Li, Zhen; Liu, Xiao-Bai; Liu, Yun-Hui; Xue, Yi-Xue; Liu, Jing; Teng, Hao; Xi, Zhuo; Yao, Yi-Long

    2016-06-01

    Previous studies have demonstrated that low-dose endothelial monocyte-activating polypeptide-II (EMAP-II) can increase blood-tumor barrier (BTB) permeability via both paracellular and transcellular pathways. In addition, we revealed that the RhoA/Rho kinase (ROCK) signaling pathway is involved in EMAP-II-induced BTB opening. This study further investigated the exact mechanisms by which the RhoA/ROCK signaling pathway affects EMAP-II-induced BTB hyperpermeability. In an in vitro BTB model, low-dose EMAP-II significantly activated phosphatidylinositol-3-kinase (PI3K) in rat brain microvascular endothelial cells (RBMECs) at 0.75 h. Pretreatment with RhoA inhibitor C3 exoenzyme or ROCK inhibitor Y-27632 completely blocked EMAP-II-induced activation of PI3K. PKC-α/β inhibitor GÖ6976 pretreatment caused no change in EMAP-II-induced activation of PI3K. Besides, pretreatment with LY294002, a specific inhibitor of PI3K, did not affect EMAP-II-induced activation of PKC-α/β. Furthermore, LY294002 pretreatment significantly diminished EMAP-II-induced changes in BTB permeability, phosphorylation of myosin light chain and cofilin, expression and distribution of tight junction-associated protein ZO-1, and actin cytoskeleton arrangement in RBMECs. In summary, this study demonstrates that low-dose EMAP-II can increase BTB permeability by activating the RhoA/ROCK/PI3K signaling pathway.

  18. ACOUSTICAL IMAGING AND MECHANICAL PROPERTIES OF SOFT ROCK AND MARINE SEDIMENTS

    SciTech Connect

    Thurman E. Scott, Jr., Ph.D.; Younane Abousleiman, Ph.D.; Musharraf Zaman, Ph.D., P.E.

    2002-11-18

    During the sixth quarter of this research project the research team developed a method and the experimental procedures for acquiring the data needed for ultrasonic tomography of rock core samples under triaxial stress conditions as outlined in Task 10. Traditional triaxial compression experiments, where compressional and shear wave velocities are measured, provide little or no information about the internal spatial distribution of mechanical damage within the sample. The velocities measured between platen-to-platen or sensor-to-sensor reflects an averaging of all the velocities occurring along that particular raypath across the boundaries of the rock. The research team is attempting to develop and refine a laboratory equivalent of seismic tomography for use on rock samples deformed under triaxial stress conditions. Seismic tomography, utilized for example in crosswell tomography, allows an imaging of the velocities within a discrete zone within the rock. Ultrasonic or acoustic tomography is essentially the extension of that field technology applied to rock samples deforming in the laboratory at high pressures. This report outlines the technical steps and procedures for developing this technology for use on weak, soft chalk samples. Laboratory tests indicate that the chalk samples exhibit major changes in compressional and shear wave velocities during compaction. Since chalk is the rock type responsible for the severe subsidence and compaction in the North Sea it was selected for the first efforts at tomographic imaging of soft rocks. Field evidence from the North Sea suggests that compaction, which has resulted in over 30 feet of subsidence to date, is heterogeneously distributed within the reservoir. The research team will attempt to image this very process in chalk samples. The initial tomographic studies (Scott et al., 1994a,b; 1998) were accomplished on well cemented, competent rocks such as Berea sandstone. The extension of the technology to weaker samples is

  19. ACOUSTICAL IMAGING AND MECHANICAL PROPERTIES OF SOFT ROCK AND MARINE SEDIMENTS

    SciTech Connect

    Thurman E. Scott, Jr., Ph.D.; Musharraf Zaman, Ph.D.; Younane Abousleiman, Ph.D.

    2001-04-01

    The oil and gas industry has encountered significant problems in the production of oil and gas from weak rocks (such as chalks and limestones) and from unconsolidated sand formations. Problems include subsidence, compaction, sand production, and catastrophic shallow water sand flows during deep water drilling. Together these cost the petroleum industry hundreds of millions of dollars annually. The goals of this first quarterly report is to document the progress on the project to provide data on the acoustic imaging and mechanical properties of soft rock and marine sediments. The project is intended to determine the geophysical (acoustic velocities) rock properties of weak, poorly cemented rocks and unconsolidated sands. In some cases these weak formations can create problems for reservoir engineers. For example, it cost Phillips Petroleum 1 billion dollars to repair of offshore production facilities damaged during the unexpected subsidence and compaction of the Ekofisk Field in the North Sea (Sulak 1991). Another example is the problem of shallow water flows (SWF) occurring in sands just below the seafloor encountered during deep water drilling operations. In these cases the unconsolidated sands uncontrollably flow up around the annulus of the borehole resulting in loss of the drill casing. The $150 million dollar loss of the Ursa development project in the U.S. Gulf Coast resulted from an uncontrolled SWF (Furlow 1998a,b; 1999a,b). The first three tasks outlined in the work plan are: (1) obtain rock samples, (2) construct new acoustic platens, (3) calibrate and test the equipment. These have been completed as scheduled. Rock Mechanics Institute researchers at the University of Oklahoma have obtained eight different types of samples for the experimental program. These include: (a) Danian Chalk, (b) Cordoba Cream Limestone, (c) Indiana Limestone, (d) Ekofisk Chalk, (e) Oil Creek Sandstone, (f) unconsolidated Oil Creek sand, and (g) unconsolidated Brazos river sand

  20. Strain localisation in mechanically layered rocks beneath detachment zones: insights from numerical modelling

    NASA Astrophysics Data System (ADS)

    Le Pourhiet, L.; Huet, B.; Labrousse, L.; Yao, K.; Agard, P.; Jolivet, L.

    2013-04-01

    We have designed a series of fully dynamic numerical simulations aimed at assessing how the orientation of mechanical layering in rocks controls the orientation of shear bands and the depth of penetration of strain in the footwall of detachment zones. Two parametric studies are presented. In the first one, the influence of stratification orientation on the occurrence and mode of strain localisation is tested by varying initial dip of inherited layering in the footwall with regard to the orientation of simple shear applied at the rigid boundary simulating a rigid hanging wall, all scaling and rheological parameter kept constant. It appears that when Mohr-Coulomb plasticity is being used, shear bands are found to localise only when the layering is being stretched. This corresponds to early deformational stages for inital layering dipping in the same direction as the shear is applied, and to later stages for intial layering dipping towards the opposite direction of shear. In all the cases, localisation of the strain after only γ=1 requires plastic yielding to be activated in the strong layer. The second parametric study shows that results are length-scale independent and that orientation of shear bands is not sensitive to the viscosity contrast or the strain rate. However, decreasing or increasing strain rate is shown to reduce the capacity of the shear zone to localise strain. In the later case, the strain pattern resembles a mylonitic band but the rheology is shown to be effectively linear. Based on the results, a conceptual model for strain localisation under detachment faults is presented. In the early stages, strain localisation occurs at slow rates by viscous shear instabilities but as the layered media is exhumed, the temperature drops and the strong layers start yielding plastically, forming shear bands and localising strain at the top of the shear zone. Once strain localisation has occured, the deformation in the shear band becomes extremely penetrative but

  1. E. coli RS2GFP Retention Mechanisms in Laboratory-Scale Fractured Rocks: A Statistical Model

    NASA Astrophysics Data System (ADS)

    Rodrigues, S. N.; Qu, J.; Dickson, S. E.

    2011-12-01

    With billions of gallons of groundwater being withdrawn every day in the US and Canada, it is imperative to understand the mechanisms which jeopardize this resource and the health of those who rely on it. Porous media aquifers have typically been considered to provide significant filtration of particulate matter (e.g. microorganisms), while the fractures in fractured rock aquifers and aquitards are considered to act as contaminant highways allowing a large fraction of pathogens to travel deep into an aquifer relatively quickly. Recent research results indicate that fractured rocks filter out more particulates than typically believed. The goal of the research presented here is to quantify the number of E. coli RS2GFP retained in a single, saturated, laboratory-scale fracture, and to relate the retention of E. coli RS2GFP to the aperture field characteristics and groundwater flow rate. To achieve this goal, physical experiments were conducted at the laboratory-scale to quantify the retention of E. coli RS2GFP through several single, saturated, dolomitic limestone fractures under a range of flow rates. These fractures were also cast with a transparent epoxy in order to visualize the transport mechanisms in the various different aperture fields. The E. coli RS2GFP is tagged with a green-fluorescent protein (GFP) that is used to obtain visualization data when excited by ultraviolet light. A series of experiments was conducted, each of which involved the release of a known number of E. coli RS2GFP at the upstream end of the fracture and measuring the effluent concentration profile. These experiments were conducted using both the natural rock and transparent cast of several different aperture fields, under a range of flow rates. The effects of different aperture field characteristics and flow rates on the retention of E. coli RS2GFP will be determined by conducting a statistical analysis of the retention data under different experimental conditions. The images captured

  2. Morphologic Interpretation of Rock Failure Mechanisms Under Uniaxial Compression Based on 3D Multiscale High-resolution Numerical Modeling

    NASA Astrophysics Data System (ADS)

    Li, Gen; Liang, Zheng-Zhao; Tang, Chun-An

    2015-11-01

    Multiscale continuous lab oratory observation of the progressive failure process has become a powerful means to reveal the complex failure mechanism of rock. Correspondingly, the representative volume element (RVE)-based models, which are capable of micro/meso- to macro-scale simulations, have been proposed, for instance, the rock failure process analysis (RFPA) program. Limited by the computational bottleneck due to the RVE size, multiscale high-resolution modeling of rock failure process can hardly be implemented, especially for three-dimensional (3D) problems. In this paper, the self-developed parallel RFPA3D code is employed to investigate the failure mechanisms and various fracture morphology of laboratory-scale rectangular prism rock specimens under unconfined uniaxial compression. The specimens consist of either heterogeneous rock with low strength or relatively homogeneous rock with high strength. The numerical simulations, such as the macroscopic fracture pattern and stress-strain responses, can reproduce the well-known phenomena of physical experiments. In particular, the 3D multiscale continuum modeling is carried out to gain new insight into the morphologic interpretation of brittle failure mechanisms, which is calibrated and validated by comparing the actual laboratory experiments and field evidence. The advantages of 3D multiscale high-resolution modeling are demonstrated by comparing the failure modes against 2D numerical predictions by other models. The parallel RVE-based modeling tool in this paper can provide an alternative way to investigate the complicated failure mechanisms of rock.

  3. An Approach for Modeling Rock Discontinuous Mechanical Behavior Under Multiphase Fluid Flow Conditions

    NASA Astrophysics Data System (ADS)

    Pan, Peng-Zhi; Rutqvist, Jonny; Feng, Xia-Ting; Yan, Fei

    2014-03-01

    In this paper, the two computer codes TOUGH2 and RDCA (for "rock discontinuous cellular automaton") are integrated for coupled hydromechanical analysis of multiphase fluid flow and discontinuous mechanical behavior in heterogeneous rock. TOUGH2 is a well-established code for geohydrological analysis involving multiphase, multicomponent fluid flow and heat transport; RDCA is a numerical model developed for simulating the nonlinear and discontinuous geomechanical behavior of rock. The RDCA incorporates the discontinuity of a fracture independently of the mesh, such that the fracture can be arbitrarily located within an element, while the fluid pressure calculated by TOUGH2 can be conveniently applied to fracture surfaces. We verify and demonstrate the coupled TOUGH-RDCA simulator by modeling a number of simulation examples related to coupled multiphase flow and geomechanical processes associated with the deep geological storage of carbon dioxide—including modeling of ground surface uplift, stress-dependent permeability, and the coupled multiphase flow and geomechanical behavior of fractures intersecting the caprock.

  4. Digital Rock Physics: Mechanical Properties of Carbonate Core Plug at Different Resolutions

    NASA Astrophysics Data System (ADS)

    Jouini, M. S.; Faisal, T. F.; Islam, A.; Chevalier, S.; Jouiad, M.; Sassi, M.

    2014-12-01

    Digital Rock Physics (DRP) is a novel technology that could be used to generate accurate, fast and cost effective special core analysis (SCAL) properties to support reservoir characterization and simulation tools. For this work, Micro-CT images at different resolutions have been used to run simulations to determine elastic properties like bulk, shear, Young's Modulus and Poisson's ratio of a dry carbonate core plug from Abu Dhabi reservoirs. Pre processing and segmentation of raw images is performed in FEI 3D visualization and analysis tool Avizo. Carbonates are characterized by a very complex pore-space structure and so a high degree of heterogeneity. Abaqus that is based on Finite Element Method is used to run 2D and 3D elastic simulations. Results will be compared by simulating the same core-plug in an alternative segmentation and FEM modeling environment used previously by Jouini & Vega et al. 2012 [1]. Acoustic wave propagation experiments at different confining pressures are performed in the laboratory Triaxial machine to determine the dynamic Young's modulus and Poisson's ratio for the same core plug. Expeirmental results are compared with numerical results. [1] Jouini, M.S. and Vega, S. 2012. Simulation of carbonate rocks elastic properties using 3D X-Ray computed tomography images based on Discrete Element Method and Finite Element Method. 46th US Rock Mechanics / Geomechanics Symposium, Chicago, Il, USA, 24-27 June 2012.

  5. Multi-element analysis of emeralds and associated rocks by k(o) neutron activation analysis

    PubMed

    Acharya; Mondal; Burte; Nair; Reddy; Reddy; Reddy; Manohar

    2000-12-01

    Multi-element analysis was carried out in natural emeralds, their associated rocks and one sample of beryl obtained from Rajasthan, India. The concentrations of 21 elements were assayed by Instrumental Neutron Activation Analysis using the k0 method (k0 INAA method) and high-resolution gamma ray spectrometry. The data reveal the segregation of some elements from associated (trapped and host) rocks to the mineral beryl forming the gemstones. A reference rock standard of the US Geological Survey (USGS BCR-1) was also analysed as a control of the method.

  6. Multi-element analysis of emeralds and associated rocks by k(o) neutron activation analysis

    PubMed

    Acharya; Mondal; Burte; Nair; Reddy; Reddy; Reddy; Manohar

    2000-12-01

    Multi-element analysis was carried out in natural emeralds, their associated rocks and one sample of beryl obtained from Rajasthan, India. The concentrations of 21 elements were assayed by Instrumental Neutron Activation Analysis using the k0 method (k0 INAA method) and high-resolution gamma ray spectrometry. The data reveal the segregation of some elements from associated (trapped and host) rocks to the mineral beryl forming the gemstones. A reference rock standard of the US Geological Survey (USGS BCR-1) was also analysed as a control of the method. PMID:11077961

  7. Numerical Investigation of Seismically Induced Rock Mass Fatigue as a Mechanism Contributing to the Progressive Failure of Deep-Seated Landslides

    NASA Astrophysics Data System (ADS)

    Gischig, Valentin; Preisig, Giona; Eberhardt, Erik

    2016-06-01

    The importance of earthquakes in triggering catastrophic failure of deep-seated landslides has long been recognized and is well documented in the literature. However, seismic waves do not only act as a trigger mechanism. They also contribute to the progressive failure of large rock slopes as a fatigue process that is highly efficient in deforming and damaging rock slopes. Given the typically long recurrence time and unpredictability of earthquakes, field-based investigations of co-seismic rock slope deformations are difficult. We present here a conceptual numerical study that demonstrates how repeated earthquake activity over time can destabilize a relatively strong rock slope by creating and propagating new fractures until the rock mass is sufficiently weakened to initiate catastrophic failure. Our results further show that the damage and displacement induced by a certain earthquake strongly depends on pre-existing damage. In fact, the damage history of the slope influences the earthquake-induced displacement as much as earthquake ground motion characteristics such as the peak ground acceleration. Because seismically induced fatigue is: (1) characterized by low repeat frequency, (2) represents a large amplitude damage event, and (3) weakens the entire rock mass, it differs from other fatigue processes. Hydro-mechanical cycles, for instance, occur at higher repeat frequencies (i.e., annual cycles), lower amplitude, and only affect limited parts of the rock mass. Thus, we also compare seismically induced fatigue to seasonal hydro-mechanical fatigue. While earthquakes can progressively weaken even a strong, competent rock mass, hydro-mechanical fatigue requires a higher degree of pre-existing damage to be effective. We conclude that displacement rates induced by hydro-mechanical cycling are indicative of the degree of pre-existing damage in the rock mass. Another indicator of pre-existing damage is the seismic amplification pattern of a slope; frequency

  8. Young Scientists Explore Rocks & Minerals. Book 11--Intermediate Level. A Good Apple Activity Book.

    ERIC Educational Resources Information Center

    DeBruin, Jerry

    Designed to develop creativity in young learners, this book contains interdisciplinary activities which focus on the theme of rocks and minerals. Activity pages are provided that can serve as front and back covers of a student booklet and the suggested activities can be duplicated for insertion between the covers resulting in a booklet for each…

  9. Ancient microbial activity recorded in fracture fillings from granitic rocks (Äspö Hard Rock Laboratory, Sweden).

    PubMed

    Heim, C; Lausmaa, J; Sjövall, P; Toporski, J; Dieing, T; Simon, K; Hansen, B T; Kronz, A; Arp, G; Reitner, J; Thiel, V

    2012-07-01

    Fracture minerals within the 1.8-Ga-old Äspö Diorite (Sweden) were investigated for fossil traces of subterranean microbial activity. To track the potential organic and inorganic biosignatures, an approach combining complementary analytical techniques of high lateral resolution was applied to drill core material obtained at -450 m depth in the Äspö Hard Rock Laboratory. This approach included polarization microscopy, time-of-flight secondary ion mass spectrometry (ToF-SIMS), confocal Raman microscopy, electron microprobe (EMP) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The fracture mineral succession, consisting of fluorite and low-temperature calcite, showed a thin (20-100 μm), dark amorphous layer lining the boundary between the two phases. Microscopic investigations of the amorphous layer revealed corrosion marks and, in places, branched tubular structures within the fluorite. Geochemical analysis showed significant accumulations of Si, Al, Mg, Fe and the light rare earth elements (REE) in the amorphous layer. In the same area, ToF-SIMS imaging revealed abundant, partly functionalized organic moieties, for example, C(x)H(y)⁺, C(x)H(y)N⁺, C(x)H(y)O⁺. The presence of such functionalized organic compounds was corroborated by Raman imaging showing bands characteristic of C-C, C-N and C-O bonds. According to its organic nature and the abundance of relatively unstable N- and O- heterocompounds, the organic-rich amorphous layer is interpreted to represent the remains of a microbial biofilm that established much later than the initial cooling of the Precambrian host rock. Indeed, δ¹³C, δ¹⁸O and ⁸⁷Sr/⁸⁶Sr isotope data of the fracture minerals and the host rock point to an association with a fracture reactivation event in the most recent geological past.

  10. Ancient microbial activity recorded in fracture fillings from granitic rocks (Äspö Hard Rock Laboratory, Sweden).

    PubMed

    Heim, C; Lausmaa, J; Sjövall, P; Toporski, J; Dieing, T; Simon, K; Hansen, B T; Kronz, A; Arp, G; Reitner, J; Thiel, V

    2012-07-01

    Fracture minerals within the 1.8-Ga-old Äspö Diorite (Sweden) were investigated for fossil traces of subterranean microbial activity. To track the potential organic and inorganic biosignatures, an approach combining complementary analytical techniques of high lateral resolution was applied to drill core material obtained at -450 m depth in the Äspö Hard Rock Laboratory. This approach included polarization microscopy, time-of-flight secondary ion mass spectrometry (ToF-SIMS), confocal Raman microscopy, electron microprobe (EMP) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The fracture mineral succession, consisting of fluorite and low-temperature calcite, showed a thin (20-100 μm), dark amorphous layer lining the boundary between the two phases. Microscopic investigations of the amorphous layer revealed corrosion marks and, in places, branched tubular structures within the fluorite. Geochemical analysis showed significant accumulations of Si, Al, Mg, Fe and the light rare earth elements (REE) in the amorphous layer. In the same area, ToF-SIMS imaging revealed abundant, partly functionalized organic moieties, for example, C(x)H(y)⁺, C(x)H(y)N⁺, C(x)H(y)O⁺. The presence of such functionalized organic compounds was corroborated by Raman imaging showing bands characteristic of C-C, C-N and C-O bonds. According to its organic nature and the abundance of relatively unstable N- and O- heterocompounds, the organic-rich amorphous layer is interpreted to represent the remains of a microbial biofilm that established much later than the initial cooling of the Precambrian host rock. Indeed, δ¹³C, δ¹⁸O and ⁸⁷Sr/⁸⁶Sr isotope data of the fracture minerals and the host rock point to an association with a fracture reactivation event in the most recent geological past. PMID:22506979

  11. Rock Finding

    ERIC Educational Resources Information Center

    Rommel-Esham, Katie; Constable, Susan D.

    2006-01-01

    In this article, the authors discuss a literature-based activity that helps students discover the importance of making detailed observations. In an inspiring children's classic book, "Everybody Needs a Rock" by Byrd Baylor (1974), the author invites readers to go "rock finding," laying out 10 rules for finding a "perfect" rock. In this way, the…

  12. Mechanisms for folding of high-grade rocks in extensional tectonic settings

    NASA Astrophysics Data System (ADS)

    Harris, Lyal B.; Koyi, Hemin A.; Fossen, Haakon

    2002-11-01

    This review of structures developed in extensional high-grade terrains, combined with results of centrifuge analogue modelling, illustrates the range of fold styles and mechanisms for folding of amphibolite to granulite facies rocks during rifting or the collapse of a thrust-thickened orogen. Several extensional fold mechanisms (such as folding within detachment shear zones) are similar to those in contractional settings. The metamorphic P- T- t path, and not fold style or mode of formation, is therefore required to determine the tectonic setting in which some folds developed. Other mechanisms such as rollover above and folding between listric normal shear zones, and folding due to isostatic adjustments during crustal thinning, are unique to extensional tectonic settings. Several mechanisms for folding during crustal extension produce structures that could easily be misinterpreted as implying regional contraction and hence lead to errors in their tectonic interpretation. It is shown that isoclinal recumbent folds refolded by open, upright folds may develop during regional extension in the deep crust. Folds with a thrust sense of asymmetry can develop due to high shear strains within an extensional detachment, or from enhanced back-rotation of layers between normal shear zones. During back-rotation folding, layers rotated into the shortening field undergo further buckle folding, and all may rotate towards orthogonality to the maximum shortening direction. This mechanism explains the presence of many transposed folds, folds with axial planar pegmatites and folds with opposite vergence in extensional terrains. Examples of folds in high-grade rocks interpreted as forming during regional extension included in this paper are from the Grenville Province of Canada, Norwegian Caledonides, Albany Mobile Belt and Leeuwin Complex of Western Australia, Ruby Mountains in the Basin and Range Province of Nevada, the Atâ Sund area of Greenland, the Napier Complex of Enderby Land

  13. Abnormal Activation of RhoA/ROCK-I Signaling in Junctional Zone Smooth Muscle Cells of Patients With Adenomyosis.

    PubMed

    Wang, S; Duan, H; Zhang, Y; Sun, F Q

    2016-03-01

    Adenomyosis (ADS) is a common estrogen-dependent gynecological disease with unknown etiology. The RhoA/Rho-kinase (ROCK) signaling pathway is involved in various cellular functions, including migration, proliferation, and smooth muscle contraction. Here we examined the potential role of this pathway in junctional zone (JZ) contraction in women with and without ADS. We demonstrated that in the normal JZ, RhoA and ROCK-I messenger RNA (mRNA) and protein expression was significantly higher in the proliferative phase of the menstrual cycle than in the secretory phase. Expression of RhoA and ROCK-I in the JZ from women with ADS was significantly higher than in the control women and showed no significant differences across the menstrual cycle. Treatment of JZ smooth muscle cells (JZSMCs) with estrogen at 0, 1, 10, or 100 nmol/L for 24 hours resulted in increased expression of RhoA, ROCK-I, and myosin light-chain (MLC) phosphorylation (p-MLC) in a dose-dependent manner. In parallel to its effects on p-MLC, estrogen-mediated, dose-dependent contraction responses in JZSMCs. Estrogen-mediated contraction in the ADS group was significantly higher than in the controls and also showed no significant differences across the menstrual cycle. These effects were suppressed in the presence of ICI 182780 or Y27632, supporting an estrogen receptor-dependent and RhoA activation-dependent mechanism. Our results indicate that the level of RhoA and ROCK-I increases in patients with ADS and the cyclic change is lost. Estrogen may affect uterine JZ contraction of ADS by enhancing RhoA/ ROCK-I signaling. PMID:26335177

  14. Abnormal Activation of RhoA/ROCK-I Signaling in Junctional Zone Smooth Muscle Cells of Patients With Adenomyosis.

    PubMed

    Wang, S; Duan, H; Zhang, Y; Sun, F Q

    2016-03-01

    Adenomyosis (ADS) is a common estrogen-dependent gynecological disease with unknown etiology. The RhoA/Rho-kinase (ROCK) signaling pathway is involved in various cellular functions, including migration, proliferation, and smooth muscle contraction. Here we examined the potential role of this pathway in junctional zone (JZ) contraction in women with and without ADS. We demonstrated that in the normal JZ, RhoA and ROCK-I messenger RNA (mRNA) and protein expression was significantly higher in the proliferative phase of the menstrual cycle than in the secretory phase. Expression of RhoA and ROCK-I in the JZ from women with ADS was significantly higher than in the control women and showed no significant differences across the menstrual cycle. Treatment of JZ smooth muscle cells (JZSMCs) with estrogen at 0, 1, 10, or 100 nmol/L for 24 hours resulted in increased expression of RhoA, ROCK-I, and myosin light-chain (MLC) phosphorylation (p-MLC) in a dose-dependent manner. In parallel to its effects on p-MLC, estrogen-mediated, dose-dependent contraction responses in JZSMCs. Estrogen-mediated contraction in the ADS group was significantly higher than in the controls and also showed no significant differences across the menstrual cycle. These effects were suppressed in the presence of ICI 182780 or Y27632, supporting an estrogen receptor-dependent and RhoA activation-dependent mechanism. Our results indicate that the level of RhoA and ROCK-I increases in patients with ADS and the cyclic change is lost. Estrogen may affect uterine JZ contraction of ADS by enhancing RhoA/ ROCK-I signaling.

  15. Fundamental Research on Percussion Drilling: Improved rock mechanics analysis, advanced simulation technology, and full-scale laboratory investigations

    SciTech Connect

    Michael S. Bruno

    2005-12-31

    This report summarizes the research efforts on the DOE supported research project Percussion Drilling (DE-FC26-03NT41999), which is to significantly advance the fundamental understandings of the physical mechanisms involved in combined percussion and rotary drilling, and thereby facilitate more efficient and lower cost drilling and exploration of hard-rock reservoirs. The project has been divided into multiple tasks: literature reviews, analytical and numerical modeling, full scale laboratory testing and model validation, and final report delivery. Literature reviews document the history, pros and cons, and rock failure physics of percussion drilling in oil and gas industries. Based on the current understandings, a conceptual drilling model is proposed for modeling efforts. Both analytical and numerical approaches are deployed to investigate drilling processes such as drillbit penetration with compression, rotation and percussion, rock response with stress propagation, damage accumulation and failure, and debris transportation inside the annulus after disintegrated from rock. For rock mechanics modeling, a dynamic numerical tool has been developed to describe rock damage and failure, including rock crushing by compressive bit load, rock fracturing by both shearing and tensile forces, and rock weakening by repetitive compression-tension loading. Besides multiple failure criteria, the tool also includes a damping algorithm to dissipate oscillation energy and a fatigue/damage algorithm to update rock properties during each impact. From the model, Rate of Penetration (ROP) and rock failure history can be estimated. For cuttings transport in annulus, a 3D numerical particle flowing model has been developed with aid of analytical approaches. The tool can simulate cuttings movement at particle scale under laminar or turbulent fluid flow conditions and evaluate the efficiency of cutting removal. To calibrate the modeling efforts, a series of full-scale fluid hammer

  16. An experimental study on fracture mechanical behavior of rock-like materials containing two unparallel fissures under uniaxial compression

    NASA Astrophysics Data System (ADS)

    Huang, Yan-Hua; Yang, Sheng-Qi; Tian, Wen-Ling; Zeng, Wei; Yu, Li-Yuan

    2016-06-01

    Strength and deformability characteristics of rock with pre-existing fissures are governed by cracking behavior. To further research the effects of pre-existing fissures on the mechanical properties and crack coalescence process, a series of uniaxial compression tests were carried out for rock-like material with two unparallel fissures. In the present study, cement, quartz sand, and water were used to fabricate a kind of brittle rock-like material cylindrical model specimen. The mechanical properties of rock-like material specimen used in this research were all in good agreement with the brittle rock materials. Two unparallel fissures (a horizontal fissure and an inclined fissure) were created by inserting steel during molding the model specimen. Then all the pre-fissured rock-like specimens were tested under uniaxial compression by a rock mechanics servo-controlled testing system. The peak strength and Young's modulus of pre-fissured specimen all first decreased and then increased when the fissure angle increased from 0° to 75°. In order to investigate the crack initiation, propagation and coalescence process, photographic monitoring was adopted to capture images during the entire deformation process. Moreover, acoustic emission (AE) monitoring technique was also used to obtain the AE evolution characteristic of pre-fissured specimen. The relationship between axial stress, AE events, and the crack coalescence process was set up: when a new crack was initiated or a crack coalescence occurred, the corresponding axial stress dropped in the axial stress-time curve and a big AE event could be observed simultaneously. Finally, the mechanism of crack propagation under microscopic observation was discussed. These experimental results are expected to increase the understanding of the strength failure behavior and the cracking mechanism of rock containing unparallel fissures.

  17. Reading the Rocks. A Fall Activity Packet for Fifth Grade.

    ERIC Educational Resources Information Center

    Jackson Community Coll., MI. Dahlem Environmental Education Center.

    This instructional packet is one of 14 school environmental education programs developed for use in the classroom and at the Dahlem Environmental Education Center (DEEC) of the Jackson Community College (Michigan). Provided in the packet are pre-trip activities, field trip activities, and post-trip activities which focus on various geological…

  18. Determination of seven noble metals in standard rock samples by means of thermal neutron activation analysis.

    PubMed

    Ayabe, M; Hirao, Y; Kimura, K

    1980-10-01

    Ruthenium, silver, rhenium, osmium, iridium, platinum and gold in standard rock samples from Geological Survey of Japan (JB-1, JB-2, JG-1 and JA-1) were determined by thermal neutron activation method with a systematic chemical separation. U.S. Geological Survey standard rocks (BCR-1 and G-2) were also analyzed to obtain an information about the accuracy and precision on the method. Generally agreement of our results with previous values for BCR-1 and G-2 was excellent. The noble metal contents in the igneous rocks from Japanese region were approximately comparable to those in the corresponding rocks from the continental region, and showed a tendency to decrease with increasing silica contents, with weak interrelations to other major elements.

  19. Numerical investigation of the hydro-mechanical contribution to seismic attenuation in damaged rocks

    NASA Astrophysics Data System (ADS)

    Pollmann, Nele; Jänicke, Ralf; Renner, Jörg; Steeb, Holger

    2016-04-01

    The investigation of hydro-mechanical processes, in particular the modeling of seismic waves in fractured porous media, is essential for the physical interpretation of data obtained from seismic exploration. Here, we specifically investigate attenuation processes in fluid-saturated porous rock containing fracture networks to identify effective hydro-mechanical properties by numerical simulation. The main purpose of this work is the characterization of the overall hydro-mechanical properties by computational homogenization. We determine an effective Skempton coefficient by investigating the fluid pressure and the solid displacement of the skeleton saturated by compressible fluids. Fracture networks are stochastically generated to mimic geological in-situ situations. The fractures are approximated as ellipses with aspect ratios up to 1/100, i.e. they constitute thin and long hydraulic conduits with high permeabilities. Simulations are designed on the material scale with and without conservation of fluid mass in the control volume. Using computational homogenization approaches, we define an effective Skempton coefficient. A range of fracture networks with different characteristic properties is studied for different varieties of fractures. On the material scale we find strongly heterogeneous pressure propagation in the fracture network and the surrounding rock, respectively. The pressure diffusion is much faster in the fracture network than in the matrix, rendering the macroscopic hydro-mechanical behavior strongly time dependent. The effective Skempton coefficient converges to an ensemble-specific instantaneous value and to 1 for long-time studies. The ultimate objective of our study is to evaluate whether constraints on the structure of fracture networks can be deduced from observations of attenuation and its frequency dependence.

  20. The Boulby Geoscience Project Underground Research Laboratory: Initial Results of a Rock Mechanics Laboratory Testing Programme

    NASA Astrophysics Data System (ADS)

    Brain, M. J.; Petley, D. N.; Rosser, N.; Lim, M.; Sapsford, M.; Barlow, J.; Norman, E.; Williams, A.; Pybus, D.

    2009-12-01

    The Boulby Mine, which is situated on the northeast coast of England, is a major source of potash, primarily for use as a fertiliser, with a secondary product of rock salt (halite), used in highway deicing. The deposits are part of the Zechstein formation and are found at depths of between c.1100 and 1135 m below sea level. The evaporite sequence also contains a range of further lithologies, including anhydrite, dolomite and a mixed evaporate deposit. From a scientific perspective the dry, uncontaminated nature of the deposits, the range of lithologies present and the high stress conditions at the mine provide a unique opportunity to observe rock deformation in situ in varying geological and stress environments. To this end the Boulby Geoscience Project was established to examine the feasibility of developing an underground research laboratory at the mine. Information regarding the mechanical properties of the strata at the Boulby Mine is required to develop our understanding of the strength and deformation behaviour of the rock over differing timescales in response to variations in the magnitude and duration of applied stresses. As such data are currently limited, we have developed a laboratory testing programme that examines the behaviour of the deposits during the application of differential compressive stresses. We present the initial results of this testing programme here. Experiments have been carried out using a high pressure Virtual Infinite Strain (VIS) triaxial apparatus (250 kN maximum axial load; 64 MPa maximum cell pressure) manufactured by GDS Instruments. Conventional compression tests under uniaxial and triaxial conditions have been undertaken to determine the effects of axial stress application rate, axial strain rate and confining pressure on behaviour and failure mechanisms. The experimental programme also includes advanced testing into time-dependent creep behaviour under constant deviatoric stress; the effects of variations in temperature and

  1. Assessment of rock mechanical properties and seismic slope stability in variably weathered layered basalts

    NASA Astrophysics Data System (ADS)

    Greenwood, William; Clark, Marin; Zekkos, Dimitrios; Von Voigtlander, Jennifer; Bateman, Julie; Lowe, Katherine; Hirose, Mitsuhito; Anderson, Suzanne; Anderson, Robert; Lynch, Jerome

    2016-04-01

    A field and laboratory experimental study was conducted to assess the influence of weathering on the mechanical properties of basalts in the region of the Kohala volcano on the island of Hawaii. Through the systematic characterization of the weathering profiles developed in different precipitation regimes, we aim to explain the regional pattern of stability of slopes in layered basalts that were observed during the 2006 Mw 6.7 Kiholo Bay earthquake. While deeper weathering profiles on the wet side of the island might be expected to promote more and larger landslides, the distribution of landslides during the Kiholo Bay earthquake did not follow this anticipated trend. Landslide frequency (defined as number of landslides divided by total area) was similar on the steepest slopes (> 50-60) for both the dry and the wet side of the study area suggesting relatively strong ground materials irrespective of weathering. The study location is ideally suited to investigate the role of precipitation, and more broadly of climate, on the mechanical properties of the local rock units because the presence of the Kohala volcano produces a significant precipitation gradient on what are essentially identical basaltic flows. Mean annual precipitation (MAP) varies by more than an order of magnitude, from 200 mm/year on the western side of the volcano to 4000 mm/year in the eastern side. We will present results of measured shear wave velocities using a seismic surface wave methodology. These results were paired with laboratory testing on selected basalt specimens that document the sample-scale shear wave velocity and unconfined compressive strength of the basaltic rocks. Shear wave velocity and unconfined strength of the rocks are correlated and are both significantly lower in weathered rocks near the ground surface than at depth. This weathering-related reduction in shear wave velocity extends to greater depths in areas of high precipitation compared to areas of lower precipitation

  2. Shrinkage Cracking: A mechanism for self-sustaining carbon mineralization reactions in olivine rocks

    NASA Astrophysics Data System (ADS)

    Zhu, W.; Fusseis, F.; Lisabeth, H. P.; Xing, T.; Xiao, X.; De Andrade, V. J. D.; Karato, S. I.

    2015-12-01

    The hydration and carbonation of olivine results in an up to ~44% increase in solid molar volume, which may choke off of fluid supply and passivate reactive surfaces, thus preventing further carbonation reactions. The carbonation of olivine has ben studied extensively in the laboratory. To date, observations from these experimental studies indicate that carbonation reaction rates generally decrease with time and the extent of carbonation is limited in olivine rocks. Field studies, however, show that 100% hydration and carbonation occur naturally in ultramafic rocks. The disagreement between the laboratory results under controlled conditions and the field observations underlines the lack of understanding of the mechanisms responsible for the self-sustaining carbonation interaction in nature. We developed a state-of-the-art pressurized hydrothermal cell that is transparent to X-rays to characterize the real-time evolution of pore geometry during fluid-rock interaction using in-situ synchrotron-based X-ray microtomography. Through a time series of high-resolution 3-dimensional images, we document the microstructural evolution of a porous olivine aggregate reacting with a sodium bicarbonate solution at elevated pressure and temperature conditions. We observed porosity increases, near constant rate of crystal growth, and pervasive reaction-induced fractures. Based on the nanometer scale tomography data, we propose that shrinkage cracking is the mechanism responsible for producing new reactive surface and keep the carbonation reaction self-sustaining in our experiment. Shrinkage cracks are commonly observed in drying mud ponds, cooling lava flows and ice wedge fields. Stretching of a contracting surface bonded to a substrate of nearly constant dimensions leads to a stress buildup in the surface layer. When the stress exceeds the tensile strength, polygonal cracks develop in the surface layer. In our experiments, the stretching mismatch between the surface and interior of

  3. The effect of long-term fluid-rock interactions on the mechanical properties of reservoir rock - a case study of the Werkendam natural CO2 analogue field

    NASA Astrophysics Data System (ADS)

    Hangx, Suzanne; Bertier, Pieter; Bakker, Elisenda; Nover, Georg; Busch, Andreas

    2015-04-01

    Geological storage of CO2 is one of the most promising technologies to rapidly reduce anthropogenic emissions of carbon dioxide. During long-term geological storage of CO2, fluid-rock interactions, induced by the formation of carbonic acid, may affect the mineralogical composition of the reservoir rock. Commonly expected reactions include the dissolution of carbonate and/or sulphate cements, as well as the reaction of primary minerals (feldspars, clays, micas) to form new, secondary phases. In order to ensure storage integrity, it is important to understand the effect of such fluid-rock interactions on the mechanical behaviour of a CO2 storage complex. However, most of these reactions are very slow, which limits the ability to study coupled chemical-mechanical processes in the lab. A possible way to circumvent long reaction times is to investigate natural CO2 analogue fields, which experienced CO2-exposure for thousands of years. In this study, we looked at the Dutch Werkendam natural CO2 field and its unreacted counterpart (Röt Fringe Sandstone, Werkendam, the Netherlands). We focussed on CO2-induced mineralogical and porosity-permeability changes, and their effect on mechanical behaviour of intact rock. Overall, CO2-exposure did not lead to drastic mineralogical changes, though markedly different porosity-permeability relationships were found for the unreacted and exposed material. The limited extent of reaction was in part the result of bitumen coatings protecting specific mineral phases from reaction. In local, mm-sized zones displaying significant anhydrite dissolution, enhanced porosity was observed. For most of the reservoir the long-term mechanical behaviour after CO2-exposure could be described by the behaviour of the unreacted sandstone, while these more 'porous' zones were significantly weaker. Simple stress path calculations predict that reservoir failure due to depletion and injection is unlikely.

  4. Determination of the Geotechnical Characteristics of Hornfelsic Rocks with a Particular Emphasis on the Correlation Between Physical and Mechanical Properties

    NASA Astrophysics Data System (ADS)

    Fereidooni, Davood

    2016-07-01

    Geotechnical characteristics and relationships between various physical and mechanical properties were assessed for eight types of hornfelsic rock collected from southern and southwestern parts of the city of Hamedan in western Iran. Rock samples were subjected to mineralogical, physical, index, and mechanical laboratory tests and found to contain quartz, feldspar, biotite, muscovite, garnet, sillimanite, kyanite, staurolite, graphite, and other fine-grained cryptocrystalline matrix materials. Samples had a porphyroblastic texture, and the mineral contents and physical properties influenced various rock characteristics. Some rock characteristics were affected by mineral content, while others were affected by porosity. Dry unit weight, primary and secondary wave velocities, and slake-durability index were noteworthy characteristics affected by mineral content, while porosity had the greatest influence on water absorption, Schmidt hardness, point load index, Brazilian tensile strength, and uniaxial compressive strength. Empirical equations describing the relationships between different rock parameters are proposed for determining the essential characteristics of rock, such as secondary wave velocity, slake-durability index, point load index, Brazilian tensile strength, and uniaxial compressive strength. On the basis of these properties, the studied rocks were classified as being strong or very strong.

  5. Mechanical Behavior of Low Porosity Carbonate Rock: From Brittle Creep to Ductile Creep.

    NASA Astrophysics Data System (ADS)

    Nicolas, A.; Fortin, J.; Gueguen, Y.

    2014-12-01

    Mechanical compaction and associated porosity reduction play an important role in the diagenesis of porous rocks. They may also affect reservoir rocks during hydrocarbon production, as the pore pressure field is modified. This inelastic compaction can lead to subsidence, cause casing failure, trigger earthquake, or change the fluid transport properties. In addition, inelastic deformation can be time - dependent. In particular, brittle creep phenomena have been deeply investigated since the 90s, especially in sandstones. However knowledge of carbonates behavior is still insufficient. In this study, we focus on the mechanical behavior of a 14.7% porosity white Tavel (France) carbonate rock (>98% calcite). The samples were deformed in a triaxial cell at effective confining pressures ranging from 0 MPa to 85 MPa at room temperature and 70°C. Experiments were carried under dry and water saturated conditions in order to explore the role played by the pore fluids. Two types of experiments have been carried out: (1) a first series in order to investigate the rupture envelopes, and (2) a second series with creep experiments. During the experiments, elastic wave velocities (P and S) were measured to infer crack density evolution. Permeability was also measured during creep experiments. Our results show two different mechanical behaviors: (1) brittle behavior is observed at low confining pressures, whereas (2) ductile behavior is observed at higher confining pressures. During creep experiments, these two behaviors have a different signature in term of elastic wave velocities and permeability changes, due to two different mechanisms: development of micro-cracks at low confining pressures and competition between cracks and microplasticity at high confining pressure. The attached figure is a summary of 20 triaxial experiments performed on Tavel limestone under different conditions. Stress states C',C* and C*' and brittle strength are shown in the P-Q space: (a) 20°C and dry

  6. Active and passive seismic methods for characterization and monitoring of unstable rock masses: field surveys, laboratory tests and modeling.

    NASA Astrophysics Data System (ADS)

    Colombero, Chiara; Baillet, Laurent; Comina, Cesare; Jongmans, Denis; Vinciguerra, Sergio

    2016-04-01

    Appropriate characterization and monitoring of potentially unstable rock masses may provide a better knowledge of the active processes and help to forecast the evolution to failure. Among the available geophysical methods, active seismic surveys are often suitable to infer the internal structure and the fracturing conditions of the unstable body. For monitoring purposes, although remote-sensing techniques and in-situ geotechnical measurements are successfully tested on landslides, they may not be suitable to early forecast sudden rapid rockslides. Passive seismic monitoring can help for this purpose. Detection, classification and localization of microseismic events within the prone-to-fall rock mass can provide information about the incipient failure of internal rock bridges. Acceleration to failure can be detected from an increasing microseismic event rate. The latter can be compared with meteorological data to understand the external factors controlling stability. On the other hand, seismic noise recorded on prone-to-fall rock slopes shows that the temporal variations in spectral content and correlation of ambient vibrations can be related to both reversible and irreversible changes within the rock mass. We present the results of the active and passive seismic data acquired at the potentially unstable granitic cliff of Madonna del Sasso (NW Italy). Down-hole tests, surface refraction and cross-hole tomography were carried out for the characterization of the fracturing state of the site. Field surveys were implemented with laboratory determination of physico-mechanical properties on rock samples and measurements of the ultrasonic pulse velocity. This multi-scale approach led to a lithological interpretation of the seismic velocity field obtained at the site and to a systematic correlation of the measured velocities with physical properties (density and porosity) and macroscopic features of the granitic cliff (fracturing, weathering and anisotropy). Continuous

  7. Deformation Mechanisms of Antigorite Serpentinite at Subduction Zone Conditions Determined from Experimentally and Naturally Deformed Rocks

    NASA Astrophysics Data System (ADS)

    Auzende, A. L.; Escartin, J.; Walte, N.; Guillot, S.; Hirth, G.; Frost, D. J.

    2014-12-01

    The rheology of serpentinite, and particularly that of antigorite-bearing rocks, is of prime importance for understanding subduction zone proceses, including decoupling between the downwelling slab and the overriding plate, exhumation of high-pressure rocks, fluids pathways and, more generally, mantle wedge dynamics. We present results from deformation-DIA experiments on antigorite serpentinite performed under conditions relevant of subduction zones (1-3.5 GPa ; 400-650°C). We complemented our study with a sample deformed in a Griggs-type apparatus at 1 GPa and 400°C (Chernak and Hirth, EPSL, 2010), and with natural samples from Cuba and the Alps deformed under blueschist/eclogitic conditions. Our observations on experimental samples of antigorite deformed within its stability field show that deformation is dominated by cataclastic flow; we can only document a minor contribution of plastic deformation. In naturally deformed samples, deformation-related plastic structures largely dominate strain accommodation, but we also document a minor contribution of brittle deformation. When dehydration occurs in experiments, plasticity increases, and is coupled to local embrittlement attributed to hydraulic fracturating due to the migration of dehydration fluids. Our results thus show that semibrittle deformation operates within and above the stability field of antigorite. We also document that the corrugated structure of antigorite has a control on the strain accommodation mechanisms under subduction conditions, with preferred inter and intra-cracking along (001) and gliding along both a and b. Deformation dominated by brittle processes, as observed in experiments, may occur during deformation at elevated (seismic?) strain rates, while plastic deformation, as observed in naturally deformed rocks, may correspond instead to low strain rates instead (aseismic creep?). We also discuss the role of antigorite rheology and mode of deformation on fluid transport.

  8. Nature and the Sign of Stress-Activated Electronic Charge Carriers in Rocks

    NASA Astrophysics Data System (ADS)

    Freund, F.

    2013-12-01

    When rocks are subjected to increasing uniaxial stress, highly mobile charge carriers become activated, which have the remarkable ability to flow out of the stressed rock volume, spreading into and through adjacent less stressed or unstressed rocks. Two basic questions arise: (i) What is the nature - and the sign - of these charge carriers and (ii) How do they exist in the rocks before being activated by stress? A large body of evidence is available that points to a positive sign, consistent with defect electrons, which (in semiconductor parlance) are called 'holes'. Holes that reside in the oxygen anion sublattice, associated with O 2sp-type energy states at the upper edge of the valence band, are called 'positive holes'. The positive holes exist in rocks in form of dormant, electrically inactive peroxy defects. Ever so slight deformation, leading to grain-grain sliding, can break the peroxy bonds and activate positive holes. Electrons that are co-activated by the same process cannot flow out of the stressed rock volume into the unstressed rocks. This leads to charge separation and, hence, to a potential difference similar to a battery voltage. In order to experimentally confirm the positive sign of the positive hole charge carriers flowing out of a given stressed rock volume, certain rules have to be obeyed. If not, unreliable results may be obtained, even negative outflow currents, seemingly inconsistent with the postulated positive sign of the positive hole charge carriers. Such errors can be avoided by taking into account that the charge outflow occurs in response to a 'battery potential', V, rapidly evolving between stressed and unstressed or less stressed parts of the rock. The current I given by Ohm's Law, I=V/R, is then limited by the internal resistance R. A second complicating factor arises from the fact that, as the positive holes are stress-activated and begin to flow out, they simultaneously recombine, returning to the dormant state. The ensuing

  9. A huge deep-seated ancient rock landslide: recognition, mechanism and stability

    NASA Astrophysics Data System (ADS)

    Tang, M. G.; Xu, Q.; Li, Y. S.; Huang, R. Q.; Zheng, G.

    2015-11-01

    The identification of deep-seated landslides is a difficult problem and its failure mechanism is a research hotspot. This paper mainly discusses a very attractive huge deep-seated ancient landslide, it is a very good case to go further research. About 15 years ago a large-scale abnormal geomorphy and geological phenomenon, containing a discontinuous stratum in output and color, was found in the new city of Fengjie, Three Gorges Project Reservoir, China. Two hypotheses for the interpretation of the abnormal phenomenon are a fault graben or a large-scale landslide. From then on continue collecting and analyzing relevant information, field investigation and test, now the results show that the fault graben, consisting of normal faults, could not have been formed under the north-south compressive structure stress of the local region. Meanwhile, a lot of unique geological features, interesting sliding trails and marks of the ancient landslide are discovered and identified in field and experiments. The deformation process and failure mechanism of the ancient landslide are clearly reappeared by a large centrifuge model experiment. Its failure mechanism can be analyzed as "creep-crack-cut". The experiment strongly confirms that it is a huge deep-seated ancient rock landslide. And the failure precursor and key factors of rock slope are discussed. At last, the stability analysis shows that the landslide as a whole is stable and the secondary landslides at the front are basically stable. The results provide a technical support for decision making of the land use planning and construction of the new city, Fengjie.

  10. Mechanical Assessment of the Drep Shield Subject to Vibratory Motion and Dynamic and Static Rock Loading

    SciTech Connect

    R.C. Quittmeyer

    2005-11-16

    The purpose of the drip shield (DS) is to divert water that may seep into emplacement drifts from contacting the waste packages, and to protect the waste packages from impact or static loading from rockfall. The objective of this document is to summarize, into one location, the results of a series of supporting engineering calculations that were developed to study the effect of static and dynamic loads on the mechanical performance of the DS. The potential DS loads are a result of: (1) Potential earthquake vibratory ground motion, and resulting interaction of the DS, waste package and pallet, and drift invert; (2) Dynamic impacts of rockfall resulting from emplacement drift damage as a result of earthquake vibratory motion; and (3) Static load of the caved rock rubble that may come to rest on the DS as a result of vibratory motion or from time-dependent yielding of the rock mass surrounding the emplacement drift. The potential mechanical failure mechanisms that may result from these loads include: (1) Overturning and/or separation of the interlocking DS segments; (2) Loss of structural integrity and stability of the DS, including excessive deformation or buckling; and (3) Localized damage to the top and side-wall plates of the DS. The scope of this document is limited to summarizing results presented in the supporting calculations in the areas of analysis of the potential for DS collapse, and determination of the damaged surface area of the DS plates. New calculations are presented to determine whether or not separation of DSs occur under vibratory motion.

  11. Mechanical behavior of low porosity carbonate rock: from brittle creep to ductile creep

    NASA Astrophysics Data System (ADS)

    Nicolas, A.; Fortin, J.; Gueguen, Y.

    2013-12-01

    Mechanical compaction and associated porosity reduction play an important role in the diagenesis of porous rocks. They may also affect reservoir rocks during hydrocarbon production, as the pore pressure field is modified. This inelastic compaction can lead to subsidence, cause casing failure, trigger earthquake, or change the fluid transport properties. In addition, inelastic deformation can be time - dependent. In particular, brittle creep phenomena have been deeply investigated since the 90s, especially in sandstones. However knowledge of carbonates behavior is still insufficient. In this experimental study, we focus on the mechanical behavior of a low porosity (9%) white Tavel (France) carbonate rock (>98% calcite) at P-Q conditions beyond the elastic domain. It has been shown that in sandstones composed of quartz, cracks are developing under these conditions. However, in carbonates, calcite minerals can meanwhile also exhibit microplasticity. The samples were deformed in the triaxial cell of the Ecole Normale Superieure de Paris at effective confining pressures ranging from 35 MPa to 85 MPa and room temperature. Experiments were carried on dry and water saturated samples to explore the role played by the pore fluids. Time dependency was investigated by a creep steps methodology: at each step, differential stress was increased rapidly and kept constant for at least 24h. During these steps elastic wave velocities (P and S) and permeability were measured continuously. Our results show two different creep behaviors: (1) brittle creep is observed at low confining pressures, whereas (2) ductile creep is observed at higher confining pressures. These two creep behaviors have a different signature in term of elastic wave velocities and permeability changes. Indeed, in the brittle domain, the primary creep is associated with a decrease of elastic wave velocities and an increase of permeability, and no secondary creep is observed. In the ductile domain, the primary creep

  12. Analysis of propagation mechanisms of stimulation-induced fractures in rocks

    NASA Astrophysics Data System (ADS)

    Krause, Michael; Renner, Joerg

    2016-04-01

    Effectivity of geothermal energy production depends crucially on the heat exchange between the penetrated hot rock and the circulating water. Hydraulic stimulation of rocks at depth intends to create a network of fractures that constitutes a large area for exchange. Two endmembers of stimulation products are typically considered, tensile hydro-fractures that propagate in direction of the largest principal stress and pre-existing faults that are sheared when fluid pressure reduces the effective normal stress acting on them. The understanding of the propagation mechanisms of fractures under in-situ conditions is still incomplete despite intensive research over the last decades. Wing-cracking has been suggested as a mechanism of fracture extension from pre-existent faults with finite length that are induced to shear. The initiation and extension of the wings is believed to be in tensile mode. Open questions concern the variability of the nominal material property controlling tensile fracture initiation and extension, the mode I facture toughness KIC, with in-situ conditions, e.g., its mean-stress dependence. We investigated the fracture-propagation mechanism in different rocks (sandstones and granites) under varying conditions mimicking those representative for geothermal systems. To determine KIC-values we performed 3-point bending experiments. We varied the confining pressure, the piston velocity, and the position of the chevron notch relative to the loading configuration. Additional triaxial experiments at a range of confining pressures were performed to study wing crack propagation from artificial flaws whose geometrical characteristics, i.e., length, width, and orientation relative to the axial load are varied. We monitored acoustic emissions to constrain the spacio-temporal evolution of the fracturing. We found a significant effect of the length of the artificial flaw and the confining pressure on wing-crack initiation but did not observe a systematic dependence

  13. Nature of extracellular signal that triggers RhoA/ROCK activation for the basal internal anal sphincter tone in humans

    PubMed Central

    Singh, Jagmohan; Kumar, Sumit; Phillips, Benjamin

    2015-01-01

    The extracellular signal that triggers activation of rho-associated kinase (RhoA/ROCK), the major molecular determinant of basal internal anal sphincter (IAS) smooth muscle tone, is not known. Using human IAS tissues, we identified the presence of the biosynthetic machineries for angiotensin II (ANG II), thromboxane A2 (TXA2), and prostaglandin F2α (PGF2α). These end products of the renin-angiotensin system (RAS) (ANG II) and arachidonic acid (TXA2 and PGF2α) pathways and their effects in human IAS vs. rectal smooth muscle (RSM) were studied. A multipronged approach utilizing immunocytochemistry, Western blot analyses, and force measurements was implemented. Additionally, in a systematic analysis of the effects of respective inhibitors along different steps of biosynthesis and those of antagonists, their end products were evaluated either individually or in combination. To further describe the molecular mechanism for the IAS tone via these pathways, we monitored RhoA/ROCK activation and its signal transduction cascade. Data showed characteristically higher expression of biosynthetic machineries of RAS and AA pathways in the IAS compared with the RSM. Additionally, specific inhibition of the arachidonic acid (AA) pathway caused ∼80% decrease in the IAS tone, whereas that of RAS lead to ∼20% decrease. Signal transduction studies revealed that the end products of both AA and RAS pathways cause increase in the IAS tone via activation of RhoA/ROCK. Both AA and RAS (via the release of their end products TXA2, PGF2α, and ANG II, respectively), provide extracellular signals which activate RhoA/ROCK for the maintenance of the basal tone in human IAS. PMID:25882611

  14. Nature of extracellular signal that triggers RhoA/ROCK activation for the basal internal anal sphincter tone in humans.

    PubMed

    Rattan, Satish; Singh, Jagmohan; Kumar, Sumit; Phillips, Benjamin

    2015-06-01

    The extracellular signal that triggers activation of rho-associated kinase (RhoA/ROCK), the major molecular determinant of basal internal anal sphincter (IAS) smooth muscle tone, is not known. Using human IAS tissues, we identified the presence of the biosynthetic machineries for angiotensin II (ANG II), thromboxane A2 (TXA2), and prostaglandin F2α (PGF2α). These end products of the renin-angiotensin system (RAS) (ANG II) and arachidonic acid (TXA2 and PGF2α) pathways and their effects in human IAS vs. rectal smooth muscle (RSM) were studied. A multipronged approach utilizing immunocytochemistry, Western blot analyses, and force measurements was implemented. Additionally, in a systematic analysis of the effects of respective inhibitors along different steps of biosynthesis and those of antagonists, their end products were evaluated either individually or in combination. To further describe the molecular mechanism for the IAS tone via these pathways, we monitored RhoA/ROCK activation and its signal transduction cascade. Data showed characteristically higher expression of biosynthetic machineries of RAS and AA pathways in the IAS compared with the RSM. Additionally, specific inhibition of the arachidonic acid (AA) pathway caused ~80% decrease in the IAS tone, whereas that of RAS lead to ~20% decrease. Signal transduction studies revealed that the end products of both AA and RAS pathways cause increase in the IAS tone via activation of RhoA/ROCK. Both AA and RAS (via the release of their end products TXA2, PGF2α, and ANG II, respectively), provide extracellular signals which activate RhoA/ROCK for the maintenance of the basal tone in human IAS.

  15. Analysis of the rock mechanics properties of volcanic tuff units from Yucca Mountain, Nevada Test Site

    SciTech Connect

    Price, R. H.

    1983-08-01

    Over two hundred fifty mechanical experiments have been run on samples of tuff from Yucca Mountain, Nevada Test Site. Cores from the Topopah Spring, Calico Hills, Bullfrog and Tram tuff units were deformed to collect data for an initial evaluation of mechanical (elastic and strength) properties of the potential horizons for emplacement of commercial nuclear wastes. The experimental conditions ranged in sample saturation from room dry to fully saturated, confining pressure from 0.1 to 20 MPa, pore pressure from 0.1 to 5 MPa, temperature from 23 to 200{sup 0}C, and strain rate from 10{sup -7} to 10{sup -2} s{sup -1}. These test data have been analyzed for variations in elastic and strength properties with changes in test conditions, and to study the effects of bulk-rock characteristics on mechanical properties. In addition to the site-specific data on Yucca Mountain tuff, mechanical test results on silicic tuff from Rainier Mesa, Nevada Test Site, are also discussed. These data both overlap and augment the Yucca Mountain tuff data, allowing more definitive conclusions to be reached, as well as providing data at some test conditions not covered by the site-specific tests.

  16. Dyke propagation and tensile fracturing at high temperature and pressure, insights from experimental rock mechanics.

    NASA Astrophysics Data System (ADS)

    Bakker, Richard; Benson, Philip; Vinciguerra, Sergio

    2014-05-01

    It is well known that magma ascends trough the crust by the process of dyking. To enable dyke emplacement, basement rocks typically fail in a mode 1 fracture, which acts as conduits for magma transport. An overpressure of the ascending magma will further open/widen the fracture and permit the fracture to propagate. In order to further understand the emplacement and arrest of dykes in the subsurface, analogue and numerical studies have been conducted. However, a number of assumptions regarding rock mechanical behaviour frequently has to be made as such data are very hard to directly measure at the pressure/temperature conditions of interest: high temperatures at relatively shallow depths. Such data are key to simulating the magma intrusion dynamics through the lithologies that underlie the volcanic edifice. Here we present a new laboratory setup, which allows us to investigate the tensile fracturing properties under both temperature and confining pressure, and the emplacement of molten material within the newly formed fracture. We have modified a traditional tri-axial test assembly setup to be able to use a Paterson type High Pressure, High Temperature deformation apparatus. Sample setup consists of cylindrical rock samples with a 22 mm diameter and a 8 mm bore at their centre, filled with a material chosen as such that it's in a liquid state at the experimental temperature and solid at room temperature to enable post-experiment analysis. The top and lower parts of the rock sample are fitted with plugs, sealing in the melt. The assembly is then placed between ceramic pistons to ensure there are no thermal gradients across the sample. The assembly is jacketed to ensure the confining medium (Ar) cannot enter the assembly. A piston is driven into the sample such that the inner conduit materials pressure is slowly increased. At some point a sufficient pressure difference between the inner and outer surfaces causes the sample to deform and fail in the tensile regime

  17. The geology and mechanics of formation of the Fort Rock Dome, Yavapai County, Arizona

    USGS Publications Warehouse

    Fuis, Gary S.

    1996-01-01

    The Fort Rock Dome, a craterlike structure in northern Arizona, is the erosional product of a circular domal uplift associated with a Precambrian shear zone exposed within the crater and with Tertiary volcanism. A section of Precambrian to Quaternary rocks is described, and two Tertiary units, the Crater Pasture Formation and the Fort Rock Creek Rhyodacite, are named. A mathematical model of the doming process is developed that is consistent with the history of the Fort Rock Dome.

  18. An experimental study of the mechanism of failure of rocks under borehole jack loading

    NASA Technical Reports Server (NTRS)

    Van, T. K.; Goodman, R. E.

    1971-01-01

    Laboratory and field tests with an experimental jack and an NX-borehole jack are reported. The following conclusions were made: Under borehole jack loading, a circular opening in a brittle solid fails by tensile fracturing when the bearing plate width is not too small. Two proposed contact stress distributions can explain the mechanism of tensile fracturing. The contact stress distribution factor is a material property which can be determined experimentally. The borehole tensile strength is larger than the rupture flexural strength. Knowing the magnitude and orientation of the in situ stress field, borehole jack test results can be used to determine the borehole tensile strength. Knowing the orientation of the in situ stress field and the flexural strength of the rock substance, the magnitude of the in situ stress components can be calculated. The detection of very small cracks is essential for the accurate determination of the failure loads which are used in the calculation of strengths and stress components.

  19. Fundamental Study on Applicability of Powder-Based 3D Printer for Physical Modeling in Rock Mechanics

    NASA Astrophysics Data System (ADS)

    Fereshtenejad, Sayedalireza; Song, Jae-Joon

    2016-06-01

    Applications of 3D printing technology become more widespread in many research fields because of its rapid development and valuable capabilities. In rock mechanics and mining engineering, this technology has the potential to become a useful tool that might help implement a number of research studies previously considered impractical. Most commercial 3D printers cannot print prototypes with mechanical properties that match precisely those of natural rock samples. Therefore, some additional enhancements are required for 3D printers to be effectively utilized for rock mechanics applications. In this study, we printed and studied specimens using a powder-based commercial ZPrinter® 450 with ZP® 150 powder and Zb® 63 binder used as raw materials. The specimens printed by this 3D printer exhibited relatively low strength and ductile behavior, implying that it needs further improvements. Hence, we focused on several ways to determine the best combination of printing options and post-processing including the effects of the printing direction, printing layer thickness, binder saturation level, and heating process on the uniaxial compressive strength (UCS) and stress-strain behavior of the printed samples. The suggested procedures have demonstrated their effectiveness by obtaining the printed samples that behave similarly to the natural rocks with low UCS. Although our optimization methods were particularly successful, further improvements are required to expand 3D printer application in the area of rock mechanics.

  20. Deformation mechanism of basic rock during long-term compression: Area of HLW repository design, Chelyabinsk District, Russia

    SciTech Connect

    Petrov, V.A.; Zviagintsev, L.I.; Poluektov, V.V.

    1996-08-01

    A combination of ultrasound, mechanical and petrographic results for long-term experimental compression of greenschist facies porphyritic andesite tuffs indicate a deformation mechanism that depends upon the mineral composition, textural-structural features of the rocks and the orientation of compression relative to the rock textures. Three dry samples of rock were investigated. Coaxial compression of a massive sample for 816 hours and a foliated sample for 1,176 hours (pressure orthogonal to foliation) is characterized by solidification when the rocks are temporarily metastable. Compressive strength of the first sample is 850 kg/cm{sup 2} and of the second one, 800 kg/cm{sup 2}. Experimentally, the rock behavior changes from a plastic to a brittle regime of deformation. In contrast, compression of the foliated sample parallel to foliation causes disintegration along the foliation within 480 hours without solidification. The rock is liable to brittle deformation and its compressive strength is 500 kg/cm{sup 2}. These results may have implications for characterization of near-field processes in connection with numerous subhorizontal zones of schistosity within the strata that are targeted for underground disposal of high-level wastes (HLW) in the Mayak radiochemical complex area.

  1. Mechanical study of the Chartreuse Fold-and-Thrust Belt: relationships between fluids overpressure and decollement within the Toarcian source-rock

    NASA Astrophysics Data System (ADS)

    Berthelon, Josselin; Sassi, William; Burov, Evgueni

    2016-04-01

    the decollement layer. In turn, with the FLAMAR geo-mechanical models it is shown that for key mechanical parameters within the Chartreuse mechanical stratigraphy (such as friction coefficient, cohesion and viscosity properties), the mechanical boundary conditions to activate, localize and propagate shear thrust in the toarcian source-rock can be found to discuss on the hydro-mechanics of the structural evolution: the very weak mechanical properties that must be attributed to the source-rock to promote the formation of a decollement tend to justify the hypothesis of high fluids pressures in it. In FLAMAR, the evolution of the toarcian source-rock mechanical properties, calibrated on the temperature of kerogen-to-gas transformation, can be introduced to allow its activation as a decollement at a burial threshold. However, without hydro-mechanical coupling, it is not possible to predict where the overpressured regions that localised these changes are positioned. As such, this work also highlights the need for a fully-coupled hydro-mechanical model to further investigate the relationship between fluids and deformations in FTB and accretionary prisms. Burov, E., Francois, T., Yamato, P., & Wolf, S. (2014). Mechanisms of continental subduction and exhumation of HP and UHP rocks. Gondwana Research, 25(2), 464-493. Faille, I., Thibaut, M., Cacas, M.-C., Havé, P., Willien, F., Wolf, S., Agelas, L., Pegaz-Fiornet, S., 2014. Modeling Fluid Flow in Faulted Basins. Oil Gas Sci. Technol. - Rev. d'IFP Energies Nouv. 69, 529-553.

  2. Porosity, mechanical strength and permeability variations associated with the presence of stylolites in carbonate rocks

    NASA Astrophysics Data System (ADS)

    Rolland, A.; Baud, P.; Heap, M. J.; Meredith, P. G.; Reuschlé, T.

    2011-12-01

    Stylolites are serrated planar features that form as a result of pressure-dissolution (i.e., due to the dissolution of calcite in stressed zones). They usually form orientated perpendicular to the maximum principal stress during their development (weight of the overburden or maximum tectonic stress). They typically form clay-enriched seams; and can sometimes reach a few hundred metres in length. The pores surrounding the stylolites are also often filled with precipitation material. Stylolites are ubiquitous features in carbonate rocks (and are also found in sandstones). Hence, they could potentially play an important role in modifying the transport and mechanical properties of their host rock. In this study, we conducted systematic porosity and permeability measurements on stylolite rich cores from limestone formations surrounding the Andra Underground Research Laboratory (URL) at Bure in the south of the Meuse district, France. Eight different limestones from the Dogger and Oxfordian ages were selected for study. The rocks are essentially composed of pure calcite and their average porosities range between 2 and 18%. Porosity measurements (performed by the water saturation technique) revealed a systematic increase of the porosity in the area approaching the stylolites, with respect to the stylolite free material. This was also visible on X-ray Computed Tomography (CT) images performed at resolutions between 4 to 40 microns. These measurements were made for two typical examples from the Dogger and Oxfordian formations. A suite of permeability measurements (using both gas and water) were performed under different hydrostatic conditions on samples specially prepared to contain either: (1) no stylolites, (2) stylolites parallel to the imposed flow and, (3) stylolites perpendicular to the imposed flow. Our new data showed that the presence of stylolites was associated, in all cases, with a moderate increase in permeability relative to stylolite-free material. A weak

  3. THERMO-HYDRO-MECHANICAL MODELING OF WORKING FLUID INJECTION AND THERMAL ENERGY EXTRACTION IN EGS FRACTURES AND ROCK MATRIX

    SciTech Connect

    Robert Podgorney; Chuan Lu; Hai Huang

    2012-01-01

    Development of enhanced geothermal systems (EGS) will require creation of a reservoir of sufficient volume to enable commercial-scale heat transfer from the reservoir rocks to the working fluid. A key assumption associated with reservoir creation/stimulation is that sufficient rock volumes can be hydraulically fractured via both tensile and shear failure, and more importantly by reactivation of naturally existing fractures (by shearing), to create the reservoir. The advancement of EGS greatly depends on our understanding of the dynamics of the intimately coupled rock-fracture-fluid-heat system and our ability to reliably predict how reservoirs behave under stimulation and production. Reliable performance predictions of EGS reservoirs require accurate and robust modeling for strongly coupled thermal-hydrological-mechanical (THM) processes. Conventionally, these types of problems have been solved using operator-splitting methods, usually by coupling a subsurface flow and heat transport simulators with a solid mechanics simulator via input files. An alternative approach is to solve the system of nonlinear partial differential equations that govern multiphase fluid flow, heat transport, and rock mechanics simultaneously, using a fully coupled, fully implicit solution procedure, in which all solution variables (pressure, enthalpy, and rock displacement fields) are solved simultaneously. This paper describes numerical simulations used to investigate the poro- and thermal- elastic effects of working fluid injection and thermal energy extraction on the properties of the fractures and rock matrix of a hypothetical EGS reservoir, using a novel simulation software FALCON (Podgorney et al., 2011), a finite element based simulator solving fully coupled multiphase fluid flow, heat transport, rock deformation, and fracturing using a global implicit approach. Investigations are also conducted on how these poro- and thermal-elastic effects are related to fracture permeability

  4. Earthquake lights and the stress-activation of positive hole charge carriers in rocks

    USGS Publications Warehouse

    St-Laurent, F.; Derr, J.S.; Freund, F.T.

    2006-01-01

    Earthquake-related luminous phenomena (also known as earthquake lights) may arise from (1) the stress-activation of positive hole (p-hole) charge carriers in igneous rocks and (2) the accumulation of high charge carrier concentrations at asperities in the crust where the stress rates increase very rapidly as an earthquake approaches. It is proposed that, when a critical charge carrier concentration is reached, the p-holes form a degenerated solid state plasma that can break out of the confined rock volume and propagate as a rapidly expanding charge cloud. Upon reaching the surface the charge cloud causes dielectric breakdown at the air-rock interface, i.e. corona discharges, accompanied by the emission of light and high frequency electromagnetic radiation. ?? 2006 Elsevier Ltd. All rights reserved.

  5. Fault damage zones in mechanically layered rocks: The effects of planar anisotropy

    NASA Astrophysics Data System (ADS)

    Misra, Santanu; Ellis, Susan; Mandal, Nibir

    2015-08-01

    This study shows how inherited strength anisotropy influences damage localization at both the tip and wall regions of a fault or fracture. We performed analogue and numerical compression experiments on transversely isotropic models with single and multiple cuts of finite length, simulating the propagation of preexisting faults and cracks in layered rock. The stress-strain curves from the analogue experiments show a change in bulk yield behavior with fault inclination and anisotropy orientation with respect to the stress direction. Earlier isotropic models demonstrated a brittle (wing fracturing) to ductile (shear-zone formation) transition as the fault angle (α) to the principal compression direction increased. The experiments with anisotropic models show patterns of damage localization change dramatically with the orientation of transversely isotropic planes (θ, measured with respect to principal extension direction). Under layer-normal (θ = 0°) and layer-parallel compression (θ = 90°), preexisting faults undergo significant reactivation when 0 < α < 90°, and fault slip eventually leads to mechanical instabilities within the anisotropic layering, causing damage zones in the tip regions. For layer-normal (θ = 0°) compression, the damage processes involve intense extensional shear localization, whereas for layer-parallel compression, contractional shear localization and tensile opening result in characteristic internal shear-band structures. In contrast, for 0 < θ < 90°, the faults undergo little or no reactivation, irrespective of α. In this case, bulk compression leads to an interlayer slip-mediated global deformation. Obliquely anisotropic models thus produce weak or no fault damage zones. We also show that the fault-parallel principal damage localized at the tips can be coupled with transversely oriented, antithetic secondary damage in the wall regions. However, secondary damage develops predominantly when θ = 90°. Field examples of fault damage

  6. A damage mechanics approach for quantifying stress changes due to brittle failure of porous rocks

    NASA Astrophysics Data System (ADS)

    Jacquey, Antoine B.; Cacace, Mauro; Blöcher, Guido; Milsch, Harald; Scheck-Wenderoth, Magdalena

    2016-04-01

    Natural fault zones or man-made injection or production of fluid impact the regional stress distribution in Earth's crust and can be responsible for localized stress discontinuities. Understanding the processes controlling fracturing of the porous rocks and mechanical behaviour of fault zones is therefore of interest for several applications including geothermal energy production. In this contribution, we will present a thermodynamically consistent visco-poroelastic damage model which can deal with the multi-scale and multi-physics nature of the physical processes controlling the deformation of porous rocks during and after brittle failure. Deformation of a porous medium is crucially influenced by the changes in the effective stress. Considering a strain-formulated yield cap and the compaction-dilation transition, three different regimes can be identified: quasi-elastic deformation, cataclastic compaction with microcracking (damage accumulation) and macroscopic brittle failure with dilation. The governing equations for deformation, damage accumulation/healing and fluid flow have been implemented in a fully-coupled finite-element-method based framework (MOOSE). The MOOSE framework provides a powerful and flexible platform to solve multiphysics problems implicitly and in a tightly coupled manner on unstructured meshes which is of interest for such non-linear context. To illustrate the model, simulation of a compaction experiment of a sandstone leading to shear failure will be presented which allows to quantify the stress drop accompanying the failure. Finally, we will demonstrate that this approach can also be used at the field scale to simulate hydraulic fracturing and assess the resulting changes in the stress field.

  7. Active cell mechanics: Measurement and theory.

    PubMed

    Ahmed, Wylie W; Fodor, Étienne; Betz, Timo

    2015-11-01

    Living cells are active mechanical systems that are able to generate forces. Their structure and shape are primarily determined by biopolymer filaments and molecular motors that form the cytoskeleton. Active force generation requires constant consumption of energy to maintain the nonequilibrium activity to drive organization and transport processes necessary for their function. To understand this activity it is necessary to develop new approaches to probe the underlying physical processes. Active cell mechanics incorporates active molecular-scale force generation into the traditional framework of mechanics of materials. This review highlights recent experimental and theoretical developments towards understanding active cell mechanics. We focus primarily on intracellular mechanical measurements and theoretical advances utilizing the Langevin framework. These developing approaches allow a quantitative understanding of nonequilibrium mechanical activity in living cells. This article is part of a Special Issue entitled: Mechanobiology.

  8. Activities report in fluid mechanics

    NASA Astrophysics Data System (ADS)

    1986-10-01

    The research conducted at the Lille Institute of Fluid Mechanics (IMFL) concerns four areas: flight mechanics, structural mechanics, aerodynamics and applied fluid mechanics. Within these four areas, these topics are discussed: characterization of the unsteady pressures on an airfoil in turbulence; adaptation of the Kalman-Rauch filtering-smoothing method to instrumented free spin tests; vulnerability of aircraft fuel tanks; water surface impact; influence of an oscillating spoiler on the surrounding aerodynamic field; gunfiring similarity theory and rules; flow around a cylinder at low Reynolds number by holographic velocimetry and laser Doppler velocimetry; compressible turbulent flow computation; and the wake of wind turbine towers are discussed.

  9. Faculty Activity to Reach Consensus and Develop the SF-ROCKS Outreach Program

    NASA Astrophysics Data System (ADS)

    Grove, K.; White, L.

    2003-12-01

    The Geosciences Department at San Francisco State University has prided itself on the excellent relationships among its faculty and students and on its proven ability to train students for careers in industry and academia. Yet, like many Geoscience departments, it recognized a need to generate higher enrollments in the undergraduate majors programs and to increase collaborations among departmental disciplines (in our case, geology, meteorology, and oceanography). To address these concerns, the department created a new outreach program that involves a majority of the faculty and that aims to increase the number of students (particularly those from underrepresented groups) who pursue a career in Geosciences at SFSU and who appreciate the role of the geosciences in their daily lives. The outreach idea was generated at a retreat of departmental faculty in January 2001. The department chair (Grove) used a classroom teaching technique to have faculty brainstorm ideas about increasing student enrollments and to reach consensus about actions to be taken. The faculty was divided into 4 groups of 3 members. Each group member spent 10 minutes brainstorming ideas and writing each idea on a post-it note. Group members then convened for 15 minutes to cluster their post-it note ideas into affinity groups. Each group subsequently had 10-15 minutes to present their ideas to the larger group, who then proceeded to decide on action items. From this activity came a clear consensus about the need for more outreach activities, and the faculty decided to submit a request for funding to a newly created NSF Geosciences program (OEDG---Opportunities for Enhancing Diversity in the Geosciences). Our proposal was successful and we received a 5-year grant to fund SF-ROCKS (Reaching out to Communities and Kids with Science in San Francisco), a program now in its second year and directed by the current department chair (White). The multi-layered program involves faculty and students from SFSU and

  10. In vivo activation of ROCK1 by insulin is impaired in skeletal muscle of humans with type 2 diabetes.

    PubMed

    Chun, Kwang-Hoon; Choi, Kang-Duk; Lee, Dae-Ho; Jung, Yoonshin; Henry, Robert R; Ciaraldi, Theodore P; Kim, Young-Bum

    2011-03-01

    To determine whether serine/threonine ROCK1 is activated by insulin in vivo in humans and whether impaired activation of ROCK1 could play a role in the pathogenesis of insulin resistance, we measured the activity of ROCK1 and the protein content of the Rho family in vastus lateralis muscle of lean, obese nondiabetic, and obese type 2 diabetic subjects. Biopsies were taken after an overnight fast and after a 3-h hyperinsulinemic euglycemic clamp. Insulin-stimulated GDR was reduced 38% in obese nondiabetic subjects compared with lean, 62% in obese diabetic subjects compared with lean, and 39% in obese diabetic compared with obese nondiabetic subjects (all comparisons P < 0.001). Insulin-stimulated IRS-1 tyrosine phosphorylation is impaired 41-48% in diabetic subjects compared with lean or obese subjects. Basal activity of ROCK1 was similar in all groups. Insulin increased ROCK1 activity 2.1-fold in lean and 1.7-fold in obese nondiabetic subjects in muscle. However, ROCK1 activity did not increase in response to insulin in muscle of obese type 2 diabetic subjects without change in ROCK1 protein levels. Importantly, insulin-stimulated ROCK1 activity was positively correlated with insulin-mediated GDR in lean subjects (P < 0.01) but not in obese or type 2 diabetic subjects. Moreover, RhoE GTPase that inhibits the catalytic activity of ROCK1 by binding to the kinase domain of the enzyme is notably increased in obese type 2 diabetic subjects, accounting for defective ROCK1 activity. Thus, these data suggest that ROCK1 may play an important role in the pathogenesis of resistance to insulin action on glucose disposal in muscle of obese type 2 diabetic subjects.

  11. HDR (Hot Dry Rock) technology transfer activities in the Clear Lake Area, California

    SciTech Connect

    Burns, K.; Potter, R.

    1990-01-01

    A large Hot Dry Rock resource has been recognized in northern California. It underlies the region extending NE of The Geysers to N of the City of Clearlake. The long-range productive potential is thousands of megawatts. The geothermal resource is heterogeneous. There are two mechanisms of heat flow occurring together. One is fluid transport, up natural zones of permeability, to outflows as surface springs. The other is conductive heat flow through impermeable rock. The temperature isotherms are thought to be nearly level surfaces, for example, the 300{degree}C isotherm is at about 8000 ft depth, with spikes'' or ridges'' occurring around narrow zones of fluid flow. While there is accessible heat at shallow depth in the naturally permeable rocks, the really substantial resource is in the impermeable rock. This is the HDR resource. The potential reservoir rocks are Franciscan greywackes and greenstones. Recorded drilling problems appear to be mainly due to intersection with serpentinites or to the effects of stimulation, so are potentially avoidable. Greywacke is favoured as a reservoir rock, and is expected to fail by brittle fracture. The water shortages in Northern California appear to be surmountable. Leakoff rates are expected to be low. Sewerage water may be available for fill and makeup. There is a possibility of combining HDR heat power production with sewerage disposal. To establish the first HDR producer in Northern California offers challenges in technology transfer. Two significant challenges will be creation of dispersed permeability in a greywacke reservoir, and pressure management in the vicinity of naturally permeable zones. A successful demonstration of HDR production technology will improve the long-term prospects for the geothermal power industry in California. 29 refs., 20 figs., 4 tabs.

  12. The mechanisms and characteristics of a complex rock-debris avalanche at the Nigeria-Cameroon border, West Africa

    NASA Astrophysics Data System (ADS)

    Igwe, Ogbonnaya; Mode, Ayonma Wilfred; Nnebedum, Okechukwu; Okonkwo, Ikenna; Oha, Ifeanyi

    2015-04-01

    We describe a rock-debris avalanche which occurred on steep, symmetrical ridges resulting from fracture-controlled erosion in the valley. The fractures were partially filled with clayey materials, probably derived from the weathering of feldspar. Major fault lines trending N-S were located less than 7 km from the landslide location. Exposed sections revealed that the basal rock units were migmatites and gneisses, while the upper section consisted of porphyritic granites. A failure of the residual clay-rich soil, composed of visible crystals of feldspar and mica, is thought to have triggered a long chain of events that led to the development of a rock-debris avalanche, which diverted the course of the rivers in the valley. The area was characterised by a shallow water table in the dry season (2 to 3 m) and this might have facilitated the formation of a slip surface at the regolith-rock interface. Field observations and laboratory analysis showed that the regolith probably failed first because of high pore pressure build-up and rapid reduction in shear resistance; this then triggered the failure of the fractured rock units. The slope movement was perpendicular to the foliation of the gneissic rocks, which probably contributed to landslide mobility. Of interest was that changing saturation level at constant relative density of about 32% resulted in either complete or limited liquefaction, indicating that the mechanism of failure depended on the moisture content of the regolith.

  13. Stress-dependent voltage offsets from polymer insulators used in rock mechanics and material testing

    NASA Astrophysics Data System (ADS)

    Carlson, G. G.; Dahlgren, R.; Vanderbilt, V. C.; Johnston, M. J.; Dunson, C.; Gray, A.; Freund, F.

    2013-12-01

    Dielectric insulators are used in a variety of laboratory settings when performing experiments in rock mechanics, petrology, and electromagnetic studies of rocks in the fields of geophysics, material science, and civil engineering. These components may be used to electrically isolate geological samples from the experimental equipment, to perform a mechanical compliance function between brittle samples and the loading equipment, to match ultrasonic transducers, or perform other functions. In many experimental configurations the insulators bear the full brunt of force applied to the sample but do not need to withstand high voltages, therefore the insulators are often thin sheets of mechanically tough polymers. From an instrument perspective, transduction from various types of mechanical perturbation has been qualitatively compared for a number of polymers [1, 2] and these error sources are readily apparent during high-impedance measurements if not mitigated. However even when following best practices, a force-dependent voltage signal still remains and its behavior is explored in this presentation. In this experiment two thin sheets (0.25 mm) of high-density polyethylene (HDPE) were set up in a stack, held alternately between three aluminum bars; this stack was placed on the platen of a 60T capacity hydraulic testing machine. The surface area, A, over which the force is applied to the PE sheets in this sandwich is roughly 40 square cm, each sheet forming a parallel-plate capacitor having roughly 320 pF [3], assuming the relative dielectric permittivity of PE is ~2.3. The outer two aluminum bars were connected to the LO input of the electrometer and the central aluminum bar was connected to the HI input of a Keithley model 617 electrometer. Once the stack is mechanically well-seated with no air gaps, the voltage offset is observed to be a linear function of the baseline voltage for a given change in applied force. For a periodically applied force of 66.7 kN the voltage

  14. Stress-Dependent Voltage Offsets From Polymer Insulators Used in Rock Mechanics and Material Testing

    NASA Technical Reports Server (NTRS)

    Carlson, G. G.; Dahlgren, Robert; Gray, Amber; Vanderbilt, V. C.; Freund, F.; Johnston, M. J.; Dunson, C.

    2013-01-01

    Dielectric insulators are used in a variety of laboratory settings when performing experiments in rock mechanics, petrology, and electromagnetic studies of rocks in the fields of geophysics,material science, and civil engineering. These components may be used to electrically isolate geological samples from the experimental equipment, to perform a mechanical compliance function between brittle samples and the loading equipment, to match ultrasonic transducers, or perform other functions. In manyexperimental configurations the insulators bear the full brunt of force applied to the sample but do not need to withstand high voltages, therefore the insulators are often thin sheets of mechanically tough polymers. From an instrument perspective, transduction from various types of mechanical perturbation has beenqualitatively compared for a number of polymers [1, 2] and these error sources are readily apparent duringhigh-impedance measurements if not mitigated. However even when following best practices, a force dependent voltage signal still remains and its behavior is explored in this presentation. In this experimenttwo thin sheets (0.25 mm) of high-density polyethylene (HDPE) were set up in a stack, held alternatelybetween three aluminum bars; this stack was placed on the platen of a 60T capacity hydraulic testingmachine. The surface area, A, over which the force is applied to the PE sheets in this sandwich is roughly 40 square cm, each sheet forming a parallel-plate capacitor having roughly 320 pF [3], assuming therelative dielectric permittivity of PE is approximately 2.3. The outer two aluminum bars were connected to the LO input ofthe electrometer and the central aluminum bar was connected to the HI input of a Keithley model 617 electrometer. Once the stack is mechanically well-seated with no air gaps, the voltage offset is observed tobe a linear function of the baseline voltage for a given change in applied force. For a periodically appliedforce of 66.7 kN the

  15. ROCK I-mediated activation of NF-κB by RhoB

    PubMed Central

    Rodriguez, Pedro L.; Sahay, Sutapa; Olabisi, Oyenike O.; Whitehead, Ian P.

    2007-01-01

    RhoB is a short-lived protein whose expression is increased by a variety of extra-cellular stimuli including UV irradiation, epidermal growth factor (EGF) and transforming growth factor β (TGF-β). Whereas most Rho proteins are modified by the covalent attachment of a geranylgeranyl group, RhoB is unique in that it can exist in either a geranylgeranylated (RhoB-GG) or a farnesylated (RhoB-F) form. Although each form is proposed to have different cellular functions, the signaling events that underlie these differences are poorly understood. Here we show that RhoB can activate NF-κB signaling in multiple cell types. Whereas RhoB-F is a potent activator of NF-κB, much weaker activation is observed for RhoB-GG, RhoA, and RhoC. NF-κB activation by RhoB is not associated with increased nuclear translocation of RelA/p65, but rather, by modification of the RelA/p65 transactivation domain. Activation of NF-κB by RhoB is dependent upon ROCK I but not PRK I. Thus, ROCK I cooperates with RhoB to activate NF-κB, and suppression of ROCK I activity by genetic or pharmacological inhibitors blocks NF-κB activation. Suppression of RhoB activity by dominant-inhibitory mutants, or siRNA, blocks NF-κB activation by Bcr, and TSG101, but not by TNFα or oncogenic Ras. Collectively, these observations suggest the existence of an endosome-associated pathway for NF-κB activation that is preferentially regulated by the farnesylated form of RhoB. PMID:17728102

  16. Deformation at Stromboli volcano (Italy) revealed by rock mechanics and structural geology

    NASA Astrophysics Data System (ADS)

    Tibaldi, A.; Corazzato, C.; Apuani, T.; Cancelli, A.

    2003-01-01

    We approach the reconstruction of the recent structural evolution of Stromboli volcano (Italy) and the analysis of the interplay between tectonics, gravity and volcanic deformation. By tying together structural, lithostratigraphic and rock mechanics data, we establish that since 100 ka BP, the edifice has faulted and jointed mainly along NE-striking planes. Faults mostly dip to the NW with normal displacement. Taking also into account the presence of a NW-trending regional least principal stress and of tectonic earthquake hypocenters inside the cone, we suggest that this fracturing can be related to the transmission of tectonic forces from the basement to the cone. Dyking concentrated along a main NE-trending weakness zone (NEZ) across the volcano summit, resembling a volcanic rift, whose geometry is governed by the tectonic field. In the past 13 ka, Stromboli experienced a reorganisation of the strain field, which was linked with the development of four sector collapses affecting the NW flank, alternating with growth phases. The tectonic strain field interplayed with dyking and fracturing related to unbuttressing along the collapse shoulders. We propose that tectonics control the geometry of dykes inside the cone and that these, in turn, contribute to destabilise the cone flanks.

  17. A numerical model of hydro-thermo-mechanical coupling in a fractured rock mass

    SciTech Connect

    Bower, K.M.

    1996-06-01

    Coupled hydro-thermo-mechanical codes with the ability to model fractured materials are used for predicting groundwater flow behavior in fractured aquifers containing thermal sources. The potential applications of such a code include the analysis of groundwater behavior within a geothermal reservoir. The capability of modeling hydro-thermo systems with a dual porosity, fracture flow model has been previously developed in the finite element code, FEHM. FEHM has been modified to include stress coupling with the dual porosity feature. FEHM has been further developed to implicitly couple the dependence of fracture hydraulic conductivity on effective stress within two dimensional, saturated aquifers containing fracture systems. The cubic law for flow between parallel plates was used to model fracture permeability. The Bartin-Bandis relationship was used to determine the fracture aperture within the cubic law. The code used a Newton Raphson iteration to implicitly solve for six unknowns at each node. Results from a model of heat flow from a reservoir to the moving fluid in a single fracture compared well with analytic results. Results of a model showing the increase in fracture flow due to a single fracture opening under fluid pressure compared well with analytic results. A hot dry rock, geothermal reservoir was modeled with realistic time steps indicating that the modified FEHM code does successfully model coupled flow problems with no convergence problems.

  18. The Alpha-Proton-X-ray Spectrometer deployment mechanism: an anthropomorphic approach to sensor placement on Martian rocks and soil

    NASA Astrophysics Data System (ADS)

    Blomquist, Richard S.

    1995-05-01

    On July 4,1997, the Mars Pathfinder spacecraft lands on Mars and starts conducting technological and scientific experiments. One experiment, the Alpha-Proton-X-ray Spectrometer, uses a sensor head placed against rocks and soil to determine their composition. To guarantee proper placement, a deployment mechanism mounted on the Mars Rover aligns the sensor head to within 20 deg of the rock and soil surfaces. In carrying out its task, the mechanism mimics the action of a human hand and arm. Consisting of a flexible wrist, a parallel link arm, a brush dc motor actuator, and a revolutionary non-pyrotechnic fail-safe release device, the mechanism correctly positions the sensor head on rocks as high as 0.29 m and on targets whose surfaces are tilted as much as 45 deg from the nominal orientation of the sensor head face. The mechanism weighs less than 0.5 kg, can withstand 100 g's, and requires less than 2.8 N x m of actuation torque. The fail-safe coupler utilizes Cerrobend, a metal alloy that melts at 60 C, to fuse the actuator and the rest of the mechanism together. A film heater wrapped around the coupler melts the metal, and Negator springs drive the mechanism into its stowed position. The fail-safe actuates using 6.75 Watts for 5 minutes in the event of an actuator failure.

  19. The Alpha-Proton-X-ray Spectrometer deployment mechanism: An anthropomorphic approach to sensor placement on Martian rocks and soil

    NASA Technical Reports Server (NTRS)

    Blomquist, Richard S.

    1995-01-01

    On July 4,1997, the Mars Pathfinder spacecraft lands on Mars and starts conducting technological and scientific experiments. One experiment, the Alpha-Proton-X-ray Spectrometer, uses a sensor head placed against rocks and soil to determine their composition. To guarantee proper placement, a deployment mechanism mounted on the Mars Rover aligns the sensor head to within 20 deg of the rock and soil surfaces. In carrying out its task, the mechanism mimics the action of a human hand and arm. Consisting of a flexible wrist, a parallel link arm, a brush dc motor actuator, and a revolutionary non-pyrotechnic fail-safe release device, the mechanism correctly positions the sensor head on rocks as high as 0.29 m and on targets whose surfaces are tilted as much as 45 deg from the nominal orientation of the sensor head face. The mechanism weighs less than 0.5 kg, can withstand 100 g's, and requires less than 2.8 N x m of actuation torque. The fail-safe coupler utilizes Cerrobend, a metal alloy that melts at 60 C, to fuse the actuator and the rest of the mechanism together. A film heater wrapped around the coupler melts the metal, and Negator springs drive the mechanism into its stowed position. The fail-safe actuates using 6.75 Watts for 5 minutes in the event of an actuator failure.

  20. Dating previously balanced rocks in seismically active parts of California and Nevada

    USGS Publications Warehouse

    Bell, J.W.; Brune, J.N.; Liu, T.; Zreda, M.; Yount, J.C.

    1998-01-01

    Precariously balanced boulders that could be knocked down by strong earthquake ground motion are found in some seismically active areas of southern California and Nevada. In this study we used two independent surface-exposure dating techniques - rock-varnish microlamination and cosmogenic 36Cl dating methodologies - to estimate minimum- and maximum-limiting ages, respectively, of the precarious boulders and by inference the elapsed time since the sites were shaken down. The results of the exposure dating indicate that all of the precarious rocks are >10.5 ka and that some may be significantly older. At Victorville and Jacumba, California, these results show that the precarious rocks have not been knocked down for at least 10.5 k.y., a conclusion in apparent conflict with some commonly used probabilistic seismic hazard maps. At Yucca Mountain, Nevada, the ages of the precarious rocks are >10.5 to >27.0 ka, providing an independent measure of the minimum time elapsed since faulting occurred on the Solitario Canyon fault.

  1. How stress and temperature conditions affect rock-fluid chemistry and mechanical deformation

    NASA Astrophysics Data System (ADS)

    Nermoen, Anders; Korsnes, Reidar; Aursjø, Olav; Madland, Merete; Kjørslevik, Trygve Alexander; Østensen, Geir

    2016-02-01

    We report the results from a series of chalk flow-through-compaction experiments performed at three effective stresses (0.5 MPa, 3.5 MPa and 12.3 MPa) and two temperatures (92° and and 130°). The results show that both stress and temperature are important to both chemical alteration and mechanical deformation. The experiments were conducted on cores drilled from the same block of outcrop chalks from the Obourg quarry within the Saint Vast formation (Mons, Belgium). The pore pressure was kept at 0.7 MPa for all experiments with a continuous flow of 0.219 M MgCl2 brine at a constant flow rate; 1 original pore volume (PV) per day. The experiments have been performed in tri-axial cells with independent control of the external stress (hydraulic pressure in the confining oil), pore pressure, temperature, and the injected flow rate. Each experiment consists of two phases; a loading phase where stress-strain dependencies are investigated (approx. 2 days), and a creep phase that lasts for more than 150-160 days. During creep, the axial deformation was logged, and the effluent samples were collected for ion chromatography analyses. Any difference between the injected and produced water chemistry gives insight into the rock-fluid interactions that occur during flow through of the core. The observed effluent concentration shows a reduction in Mg2+, while the Ca2+ concentration is increased. This, together with SEM-EDS analysis, indicates that magnesium-bearing mineral phases are precipitated leading to dissolution of calcite, an observation . This is in-line with other flow-through experiments reported earlier. The observed dissolution and precipitation are sensitive to the effective stress and test temperature. Typically. H, higher stress and temperature lead to increased concentration differences of Mg2+ and Ca2+ concentration changes.. The observed strain can be partitioned additively into a mechanical and chemical driven component.

  2. Computational method for thermoviscoelasticity with application to rock mechanics. [Ph. D. Thesis

    SciTech Connect

    Lee, S.C.

    1984-01-01

    Large-scale numerical computations associated with rock mechanics problems have required efficient and economical models for predicting temperature, stress, failure, and deformed structural configuration under various loading conditions. To meet this requirement, the complex dependence of the properties of geological materials on the time and temperature is modified to yield a reduced time scale as a function of time and temperature under the thermorheologically simple material (TSM) postulate. The thermorheologically linear concept is adopted in the finite element formulation by uncoupling thermal and mechanical responses. The thermal responses, based on transient heat conduction or convective-diffusion, are formulated by using the two-point recurrence scheme and the upwinding scheme, respectively. An incremental solution procedure with the implicit time stepping scheme is proposed for the solution of the thermoviscoelastic response. The proposed thermoviscoelastic solution algorithm is based on the uniaxial creep experimental data and the corresponding temperature shift functions, and is intended to minimize computational efforts by allowing large time step size with stable solutions. A thermoelastic fracture formulation is also presented by introducing the degenerate quadratic isoparametric singular element for the thermally-induced line crack problems. The stress intensity factors are computed by use of the displacement method. Efficiency of the presented formulation and solution algorithm is initially demonstrated by comparison with other available solutions for a variety of problems. Subsequent field applications are made to simulate the post-burn and post-repose phases of an underground coal conversion (UCC) experiment and in-situ nuclear waste disposal management problems. 137 references, 48 figures, 6 tables.

  3. Effect of chemical environment and rock composition on fracture mechanics properties of reservoir lithologies in context of CO2 sequestration

    NASA Astrophysics Data System (ADS)

    Major, J. R.; Eichhubl, P.; Callahan, O. A.

    2015-12-01

    The coupled chemical and mechanical response of reservoir and seal rocks to injection of CO2 have major implications on the short and long term security of sequestered carbon. Many current numerical models evaluating behavior of reservoirs and seals during and after CO2 injection in the subsurface consider chemistry and mechanics separately and use only simple mechanical stability criteria while ignoring time-dependent failure parameters. CO2 injection irreversibly alters the subsurface chemical environment which can then affect geomechanical properties on a range of time scales by altering rock mineralogy and cements through dissolution, remobilization, and precipitation. It has also been documented that geomechanical parameters such as fracture toughness (KIC) and subcritical index (SCI) are sensitive to chemical environment. Double torsion fracture mechanics testing of reservoir lithologies under controlled environmental conditions relevant to CO2 sequestration show that chemical environment can measurably affect KIC and SCI. This coupled chemical-mechanical behavior is also influenced by rock composition, grains, amount and types of cement, and fabric. Fracture mechanics testing of the Aztec Sandstone, a largely silica-cemented, subarkose sandstone demonstrate it is less sensitive to chemical environment than Entrada Sandstone, a silty, clay-rich sandstone. The presence of de-ionized water lowers KIC by approximately 20% and SCI 30% in the Aztec Sandstone relative to tests performed in air, whereas the Entrada Sandstone shows reductions on the order of 70% and 90%, respectively. These results indicate that rock composition influences the chemical-mechanical response to deformation, and that the relative chemical reactivity of target reservoirs should be recognized in context of CO2 sequestration. In general, inert grains and cements such as quartz will be less sensitive to the changing subsurface environment than carbonates and clays.

  4. Rock mechanics observations pertinent to the rheology of the continental lithosphere and the localization of strain along shear zones

    USGS Publications Warehouse

    Kirby, S.H.

    1985-01-01

    Emphasized in this paper are the deformation processes and rheologies of rocks at high temperatures and high effective pressures, conditions that are presumably appropriate to the lower crust and upper mantle in continental collision zones. Much recent progress has been made in understanding the flexure of the oceanic lithosphere using rock-mechanics-based yield criteria for the inelastic deformations at the top and base. At mid-plate depths, stresses are likely to be supported elastically because bending strains and elastic stresses are low. The collisional tectonic regime, however, is far more complex because very large permanent strains are sustained at mid-plate depths and this requires us to include the broad transition between brittle and ductile flow. Moreover, important changes in the ductile flow mechanisms occur at the intermediate temperatures found at mid-plate depths. Two specific contributions of laboratory rock rheology research are considered in this paper. First, the high-temperature steady-state flow mechanisms and rheology of mafic and ultramafic rocks are reviewed with special emphasis on olivine and crystalline rocks. Rock strength decreases very markedly with increases in temperature and it is the onset of flow by high temperature ductile mechanisms that defines the base of the lithosphere. The thickness of the continental lithosphere can therefore be defined by the depth to a particular isotherm Tc above which (at geologic strain rates) the high-temperature ductile strength falls below some arbitrary strength isobar (e.g., 100 MPa). For olivine Tc is about 700??-800??C but for other crustal silicates, Tc may be as low as 400??-600??C, suggesting that substantial decoupling may take place within thick continental crust and that strength may increase with depth at the Moho, as suggested by a number of workers on independent grounds. Put another way, the Moho is a rheological discontinuity. A second class of laboratory observations pertains to

  5. Role of PKC and RhoA/ROCK pathways in the spontaneous phasic activity in the rectal smooth muscle.

    PubMed

    Singh, Jagmohan; Rattan, Satish

    2013-04-15

    The role of PKC and RhoA/ROCK pathways in the phasic activities in the rectal smooth muscles (RSM) in the basal state is not known. We examined this issue by determining the effects of PKC inhibitors (calphostin C and Gö-6850) and a ROCK inhibitor (Y-27632) on the slow-rate (~3/min) and fast-rate (~25/min) phasic activities. We also examined the corresponding signal transduction cascades and the PKC and ROCK enzymatic activities in the RSM in the basal state. PKC inhibition with calphostin C and Gö-6850 (10(-5) M) caused a significant decrease (~25%) in slow-rate (but not fast-rate) phasic activity (monitored by frequency and amplitude of contractions) of the RSM. Conversely, ROCK inhibition with Y-27632 (10(-5) M) caused a significant decrease not only in slow-rate, but also fast-rate, phasic activity caused by ROCK inhibition in the RSM. Western blot analysis revealed that the PKC inhibition-induced decrease in RSM phasic activity was associated with decreases in PKCα translocation, phosphorylated (Thr(38)) PKC-potentiated inhibitor (CPI-17), and phosphorylated (Thr(18)/Ser(19)) 20-kDa myosin regulatory light chain. Conversely, decreases in the phasic activity in the RSM by ROCK inhibition were accompanied by the additional decrease in phosphorylated (Thr(696)) myosin phosphatase target subunit 1. Data show that while PKC and RhoA/ROCK pathways play a significant role in slow-rate high-amplitude spontaneous phasic activity, only the RhoA/ROCK pathway primarily mediates fast-rate low-amplitude phasic activity, in the RSM. Such knowledge is important in the understanding of the pathophysiology of large intestinal motility disorders. Relative contributions of the PKC vs. the RhoA/ROCK pathway in the phasic activity remain to be determined.

  6. ROCK activity regulates functional tight junction assembly during blastocyst formation in porcine parthenogenetic embryos

    PubMed Central

    Kwon, Jeongwoo

    2016-01-01

    The Rho-associated coiled-coil-containing protein serine/threonine kinases 1 and 2 (ROCK1 and ROCK2) are Rho subfamily GTPase downstream effectors that regulate cell migration, intercellular adhesion, cell polarity, and cell proliferation by stimulating actin cytoskeleton reorganization. Inhibition of ROCK proteins affects specification of the trophectoderm (TE) and inner cell mass (ICM) lineages, compaction, and blastocyst cavitation. However, the molecules involved in blastocyst formation are not known. Here, we examined developmental competence and levels of adherens/tight junction (AJ/TJ) constituent proteins, such as CXADR, OCLN, TJP1, and CDH1, as well as expression of their respective mRNAs, after treating porcine parthenogenetic four-cell embryos with Y-27632, a specific inhibitor of ROCK, at concentrations of 0, 10, 20, 100 µM for 24 h. Following this treatment, the blastocyst development rates were 39.1, 20.7, 10.0, and 0% respectively. In embryos treated with 20 µM treatment, expression levels of CXADR, OCLN, TJP1, and CDH1 mRNA and protein molecules were significantly reduced (P < 0.05). FITC-dextran uptake assay revealed that the treatment caused an increase in TE TJ permeability. Interestingly, the majority of the four-cell and morula embryos treated with 20 µM Y-27643 for 24 h showed defective compaction and cavitation. Taken together, our results indicate that ROCK activity may differentially affect assembly of AJ/TJs as well as regulate expression of genes encoding junctional proteins. PMID:27077008

  7. Coupled hydro-mechanical processes in crytalline rock and ininduratedand plastic clays: A comparative discussion

    SciTech Connect

    Tsang, Chin-Fu; Blumling, Peter; Bernier, Frederic

    2006-02-15

    This paper provides a comparative discussion of coupledhydromechanical processes in three different geological formations:crystalline rock, plastic clay, and indurated clay. First, the importantprocesses and associated property characteristics in the three rock typesare discussed. Then, one particular hydromechanical coupling is broughtup for detailed consideration, that of pore pressure changes in nearbyrock during tunnel excavation. Three field experiments in the three rocktypes are presented and their results are discussed. It is shown that themain physical processes are common to all three rock types, but with verydifferent time constants. The different issues raised by these cases arepointed out, and the transferable lessons learned are identified. Suchcross fertilization and simultaneous understanding of coupled processesin three very different rock types help to greatly enhance confidence inthe state of science in this field.

  8. Systematic study of the effects of mass and time scaling techniques applied in numerical rock mechanics simulations

    NASA Astrophysics Data System (ADS)

    Heinze, Thomas; Jansen, Gunnar; Galvan, Boris; Miller, Stephen A.

    2016-08-01

    Numerical modeling is a well established tool in rock mechanics studies investigating a wide range of problems. Implicit methods for solving linear equations have the advantage of being unconditionally stable, while explicit methods, although limited by the time step, are often used because of their limited memory demand, their scalability in parallel computing, and simple implementation of complex boundary conditions. In numerical modeling of explicit elastoplastic dynamics where the time step is limited by the material density, mass scaling techniques can be used to overcome this limit and significantly reduce computation time. While often used, the effect of mass and time scaling and how it may influence the numerical results is rarely-mentioned in publications, and choosing the right scaling technique is typically performed by trial and error. To our knowledge, no systematic studies have addressed how mass scaling might affect the numerical results. In this paper, we present results from an extensive and systematic study of the influence of mass and time scaling on the behavior of a variety of rock-mechanical models. We employ a finite difference scheme to model uniaxial and biaxial compression experiments using different mass and time scaling factors, and with physical models of increasing complexity up to a cohesion-weakening frictional-strengthening model (CWFS). We also introduce a normalized energy ratio to assist analyzing mass scaling effects. We find the tested models to be less sensitive to time scaling than to mass scaling, so mass scaling has higher potential for decreasing computational costs. However, we also demonstrate that mass scaling may lead to quantitatively wrong results, so care must be taken in interpreting stress values when mass scaling is used in complicated rock mechanics simulations. Mass scaling significantly influences the stress-strain response of numerical rocks because mass scaling acts as an artificial hardening agent on rock

  9. Origin and accumulation mechanisms of petroleum in the Carboniferous volcanic rocks of the Kebai Fault zone, Western Junggar Basin, China

    NASA Astrophysics Data System (ADS)

    Chen, Zhonghong; Zha, Ming; Liu, Keyu; Zhang, Yueqian; Yang, Disheng; Tang, Yong; Wu, Kongyou; Chen, Yong

    2016-09-01

    The Kebai Fault zone of the West Junggar Basin in northwestern China is a unique region to gain insights on the formation of large-scale petroleum reservoirs in volcanic rocks of the western Central Asian Orogenic Belt. Carboniferous volcanic rocks are widespread in the Kebai Fault zone and consist of basalt, basaltic andesite, andesite, tuff, volcanic breccia, sandy conglomerate and metamorphic rocks. The volcanic oil reservoirs are characterized by multiple sources and multi-stage charge and filling history, characteristic of a complex petroleum system. Geochemical analysis of the reservoir oil, hydrocarbon inclusions and source rocks associated with these volcanic rocks was conducted to better constrain the oil source, the petroleum filling history, and the dominant mechanisms controlling the petroleum accumulation. Reservoir oil geochemistry indicates that the oil contained in the Carboniferous volcanic rocks of the Kebai Fault zone is a mixture. The oil is primarily derived from the source rock of the Permian Fengcheng Formation (P1f), and secondarily from the Permian Lower Wuerhe Formation (P2w). Compared with the P2w source rock, P1f exhibits lower values of C19 TT/C23 TT, C19+20TT/ΣTT, Ts/(Ts + Tm) and ααα-20R sterane C27/C28 ratios but higher values of TT C23/C21, HHI, gammacerane/αβ C30 hopane, hopane (20S) C34/C33, C29ββ/(ββ + αα), and C29 20S/(20S + 20R) ratios. Three major stages of oil charge occurred in the Carboniferous, in the Middle Triassic, Late Triassic to Early Jurassic, and in the Middle Jurassic to Late Jurassic periods, respectively. Most of the oil charged during the first stage was lost, while moderately and highly mature oils were generated and accumulated during the second and third stages. Oil migration and accumulation in the large-scale stratigraphic reservoir was primarily controlled by the top Carboniferous unconformity with better porosity and high oil enrichment developed near the unconformity. Secondary dissolution

  10. Protein kinase C activation disrupts epithelial apical junctions via ROCK-II dependent stimulation of actomyosin contractility

    PubMed Central

    Ivanov, Andrei I; Samarin, Stanislav N; Bachar, Moshe; Parkos, Charles A; Nusrat, Asma

    2009-01-01

    Background Disruption of epithelial cell-cell adhesions represents an early and important stage in tumor metastasis. This process can be modeled in vitro by exposing cells to chemical tumor promoters, phorbol esters and octylindolactam-V (OI-V), known to activate protein kinase C (PKC). However, molecular events mediating PKC-dependent disruption of epithelial cell-cell contact remain poorly understood. In the present study we investigate mechanisms by which PKC activation induces disassembly of tight junctions (TJs) and adherens junctions (AJs) in a model pancreatic epithelium. Results Exposure of HPAF-II human pancreatic adenocarcinoma cell monolayers to either OI-V or 12-O-tetradecanoylphorbol-13-acetate caused rapid disruption and internalization of AJs and TJs. Activity of classical PKC isoenzymes was responsible for the loss of cell-cell contacts which was accompanied by cell rounding, phosphorylation and relocalization of the F-actin motor nonmuscle myosin (NM) II. The OI-V-induced disruption of AJs and TJs was prevented by either pharmacological inhibition of NM II with blebbistatin or by siRNA-mediated downregulation of NM IIA. Furthermore, AJ/TJ disassembly was attenuated by inhibition of Rho-associated kinase (ROCK) II, but was insensitive to blockage of MLCK, calmodulin, ERK1/2, caspases and RhoA GTPase. Conclusion Our data suggest that stimulation of PKC disrupts epithelial apical junctions via ROCK-II dependent activation of NM II, which increases contractility of perijunctional actin filaments. This mechanism is likely to be important for cancer cell dissociation and tumor metastasis. PMID:19422706

  11. Coseismic landslides reveal near-surface rock strength in a high-relief tectonically active setting

    USGS Publications Warehouse

    Gallen, Sean F; Clark, Marin K; Godt, Jonathan W.

    2014-01-01

    We present quantitative estimates of near-surface rock strength relevant to landscape evolution and landslide hazard assessment for 15 geologic map units of the Longmen Shan, China. Strength estimates are derived from a novel method that inverts earthquake peak ground acceleration models and coseismic landslide inventories to obtain material proper- ties and landslide thickness. Aggregate rock strength is determined by prescribing a friction angle of 30° and solving for effective cohesion. Effective cohesion ranges are from 70 kPa to 107 kPa for 15 geologic map units, and are approximately an order of magnitude less than typical laboratory measurements, probably because laboratory tests on hand-sized specimens do not incorporate the effects of heterogeneity and fracturing that likely control near-surface strength at the hillslope scale. We find that strength among the geologic map units studied varies by less than a factor of two. However, increased weakening of units with proximity to the range front, where precipitation and active fault density are the greatest, suggests that cli- matic and tectonic factors overwhelm lithologic differences in rock strength in this high-relief tectonically active setting.

  12. The influence of fluids on the long-term deformation and strength of a porous rock: hydro-mechanical effect versus physiochemical effects

    NASA Astrophysics Data System (ADS)

    Grgic, D.

    2011-12-01

    influence. Both acoustic (statistical analysis of number and energy of Acoustic Emissions) and mechanical (strength, deformation) properties were investigated during these laboratory tests. It resulted from this study that AE activity and dilatant inelastic volumetric strain increase strongly and Young's modulus and b-value of the Gutenberg-Richter law (i.e., the relative number of large-amplitude events increases) decrease strongly with the increase in water and ethanol saturation and as the rock approaches failure. This indicates that micro-cracking plays an important role in the creep process. In addition, the long-term strength increases and the deformation and acoustic activity decrease as the chemical influence of the interstitial fluid decreases, i.e., the rock is weaker in the presence of liquid water. It was shown that fluids accelerate static fatigue of porous rocks through hydro-mechanical coupling and physiochemical mechanisms (mainly subcritical stress corrosion cracking), the latter being the dominant fluid effect.

  13. Dissolution of bedded rock salt: A seismic profile across the active eastern margin of the Hutchinson Salt Member, central Kansas

    USGS Publications Warehouse

    Anderson, N.L.; Hopkins, J.; Martinez, A.; Knapp, R.W.; Macfarlane, P.A.; Watney, W.L.; Black, R.

    1994-01-01

    Since late Tertiary, bedded rock salt of the Permian Hutchinson Salt Member has been dissolved more-or-less continuously along its active eastern margin in central Kansas as a result of sustained contact with unconfined, undersaturated groundwater. The associated westward migration of the eastern margin has resulted in surface subsidence and the contemporaneous sedimentation of predominantly valley-filling Quarternary alluvium. In places, these alluvium deposits extend more than 25 km to the east of the present-day edge of the main body of contiguous rock salt. The margin could have receded this distance during the past several million years. From an environmental perspective, the continued leaching of the Hutchinson Salt is a major concern. This predominantly natural dissolution occurs in a broad zone across the central part of the State and adversely affects groundwater and surface-water quality as nonpoint source pollution. Significant surface subsidence occurs as well. Most of these subsidence features have formed gradually; others developed in a more catastrophic manner. The latter in particular pose real threats to roadways, railways, and buried oil and gas pipelines. In an effort to further clarify the process of natural salt dissolution in central Kansas and with the long-term goal of mitigating the adverse environmental affects of such leaching, the Kansas Geological Survey acquired a 4-km seismic profile across the eastern margin of the Hutchinson Salt in the Punkin Center area of central Kansas. The interpretation of these seismic data (and supporting surficial and borehole geologic control) is consistent with several hypotheses regarding the process and mechanisms of dissolution. More specifically these data support the theses that: 1. (1) Dissolution along the active eastern margin of the Hutchinson Salt Member was initiated during late Tertiary. Leaching has resulted in the steady westward migration of the eastern margin, surface subsidence, and the

  14. Dissolution of bedded rock salt: A seismic profile across the active eastern margin of the Hutchinson Salt Member, central Kansas

    NASA Astrophysics Data System (ADS)

    Anderson, Neil L.; Hopkins, John; Martinez, Alex; Knapp, Ralph W.; Macfarlane, P. Allan; Watney, W. Lynn; Black, Ross

    1994-06-01

    Since late Tertiary, bedded rock salt of the Permian Hutchinson Salt Member has been dissolved more-or-less continuously along its active eastern margin in central Kansas as a result of sustained contact with unconfined, undersaturated groundwater. The associated westward migration of the eastern margin has resulted in surface subsidence and the contemporaneous sedimentation of predominantly valley-filling Quarternary alluvium. In places, these alluvium deposits extend more than 25 km to the east of the present-day edge of the main body of contiguous rock salt. The margin could have receded this distance during the past several million years. From an environmental perspective, the continued leaching of the Hutchinson Salt is a major concern. This predominantly natural dissolution occurs in a broad zone across the central part of the State and adversely affects groundwater and surface-water quality as nonpoint source pollution. Significant surface subsidence occurs as well. Most of these subsidence features have formed gradually; others developed in a more catastrophic manner. The latter in particular pose real threats to roadways, railways, and buried oil and gas pipelines. In an effort to further clarify the process of natural salt dissolution in central Kansas and with the long-term goal of mitigating the adverse environmental affects of such leaching, the Kansas Geological Survey acquired a 4-km seismic profile across the eastern margin of the Hutchinson Salt in the Punkin Center area of central Kansas. The interpretation of these seismic data (and supporting surficial and borehole geologic control) is consistent with several hypotheses regarding the process and mechanisms of dissolution. More specifically these data support the theses that: (1) Dissolution along the active eastern margin of the Hutchinson Salt Member was initiated during late Tertiary. Leaching has resulted in the steady westward migration of the eastern margin, surface subsidence, and the

  15. Leaching of boron, arsenic and selenium from sedimentary rocks: II. pH dependence, speciation and mechanisms of release.

    PubMed

    Tabelin, Carlito Baltazar; Hashimoto, Ayaka; Igarashi, Toshifumi; Yoneda, Tetsuro

    2014-03-01

    Sedimentary rocks excavated in Japan from road- and railway-tunnel projects contain relatively low concentrations of hazardous trace elements like boron (B), arsenic (As) and selenium (Se). However, these seemingly harmless waste rocks often produced leachates with concentrations of hazardous trace elements that exceeded the environmental standards. In this study, the leaching behaviors and release mechanisms of B, As and Se were evaluated using batch leaching experiments, sequential extraction and geochemical modeling calculations. The results showed that B was mostly partitioned with the residual/crystalline phase that is relatively stable under normal environmental conditions. In contrast, the majority of As and Se were associated with the exchangeable and organics/sulfides phases that are unstable under oxidizing conditions. Dissolution of water-soluble phases controlled the leaching of B, As and Se from these rocks in the short term, but pyrite oxidation, calcite dissolution and adsorption/desorption reactions became more important in the long term. The mobilities of these trace elements were also strongly influenced by the pH of the rock-water system. Although the leaching of Se only increased in the acidic region, those of B and As were enhanced under both acidic and alkaline conditions. Under strongly acidic conditions, the primarily release mechanism of B, As and Se was the dissolution of mineral phases that incorporated and/or adsorbed these elements. Lower concentrations of these trace elements in the circumneutral pH range could be attributed to their strong adsorption onto minerals like Al-/Fe-oxyhydroxides and clays, which are inherently present and/or precipitated in the rock-water system. The leaching of As and B increased under strongly alkaline conditions because of enhanced desorption and pyrite oxidation while that of Se remained minimal due to its adsorption onto Fe-oxyhydroxides and co-precipitation with calcite.

  16. Leaching of boron, arsenic and selenium from sedimentary rocks: II. pH dependence, speciation and mechanisms of release.

    PubMed

    Tabelin, Carlito Baltazar; Hashimoto, Ayaka; Igarashi, Toshifumi; Yoneda, Tetsuro

    2014-03-01

    Sedimentary rocks excavated in Japan from road- and railway-tunnel projects contain relatively low concentrations of hazardous trace elements like boron (B), arsenic (As) and selenium (Se). However, these seemingly harmless waste rocks often produced leachates with concentrations of hazardous trace elements that exceeded the environmental standards. In this study, the leaching behaviors and release mechanisms of B, As and Se were evaluated using batch leaching experiments, sequential extraction and geochemical modeling calculations. The results showed that B was mostly partitioned with the residual/crystalline phase that is relatively stable under normal environmental conditions. In contrast, the majority of As and Se were associated with the exchangeable and organics/sulfides phases that are unstable under oxidizing conditions. Dissolution of water-soluble phases controlled the leaching of B, As and Se from these rocks in the short term, but pyrite oxidation, calcite dissolution and adsorption/desorption reactions became more important in the long term. The mobilities of these trace elements were also strongly influenced by the pH of the rock-water system. Although the leaching of Se only increased in the acidic region, those of B and As were enhanced under both acidic and alkaline conditions. Under strongly acidic conditions, the primarily release mechanism of B, As and Se was the dissolution of mineral phases that incorporated and/or adsorbed these elements. Lower concentrations of these trace elements in the circumneutral pH range could be attributed to their strong adsorption onto minerals like Al-/Fe-oxyhydroxides and clays, which are inherently present and/or precipitated in the rock-water system. The leaching of As and B increased under strongly alkaline conditions because of enhanced desorption and pyrite oxidation while that of Se remained minimal due to its adsorption onto Fe-oxyhydroxides and co-precipitation with calcite. PMID:24370699

  17. Wing Rock Motion and its Flow Mechanism over a Chined-Body Configuration

    NASA Astrophysics Data System (ADS)

    Wang, Yankui; Li, Qian; Shi, Wei

    2015-11-01

    Wing rock motion is one kind of uncommanded oscillation around the body axis over the most of the aircraft at enough high angle of attack and has a strong threat to the flight safety. The purpose of this paper is to investigate the wing rock motion over a typical body-wing configuration with a chined fuselage at fixed angle of attack firstly and four kinds of wing rock motion are revealed based on the flow phenomena, namely non-oscillation, lateral deflection, limit-cycle oscillation and irregular oscillation. Simultaneously, some special relationship between the wing rock motion and the flow over the chined body configuration are discussed. In addition, the evolution of wing rock motion and its corresponding flows when the model undergoes pitching up are also given out. All the experiments have been conducted in a low-speed wind tunnel at a Reynolds number of 1.87*10E5 and angle of attack from 0deg to 65deg. National Natural Science Foundation of China(11472028) and Open fund from State Key Laboratory of Aerodynamics.

  18. Applicability of failure criteria and empirical relations of mechanical rock properties from outcrop analogue samples for wellbore stability analyses

    NASA Astrophysics Data System (ADS)

    Reyer, D.; Philipp, S. L.

    2013-12-01

    Knowledge of failure criteria, Young's modulus and uniaxial and tensile strengths, are important to avoid borehole instabilities and adapt the drilling plan on rock mechanical conditions. By this means, a considerable reduction of the total drilling costs can be achieved. This is desirable to enlarge the profit margin of geothermal projects which is rather small compared with hydrocarbon projects. Because core material is rare we aim at predicting in situ rock properties from outcrop analogue samples which are easy and cheap to provide. The comparability of properties determined from analogue samples with samples from depths is analysed by performing conventional triaxial tests, uniaxial compressive strength tests and Brazilian tests of both quarry and equivalent core samples. Equivalent means that the quarry sample is of the same stratigraphic age and of comparable sedimentary facies and composition as the associated core sample. We determined the parameters uniaxial compressive strength (UCS), Young's modulus, and tensile strength for 35 rock samples from quarries and 14 equivalent core samples from the North German Basin. A subgroup of these samples, consisting of one volcanic rock sample, three sandstone and three carbonate samples, was used for triaxial tests. In all cases, comparability of core samples with quarry samples is evaluated using thin section analyses. For UCS versus Young's modulus and tensile strengths, linear- and non-linear regression analyses were performed. We repeat regression separately for clastic rock samples or carbonate rock samples only as well as for quarry samples or core samples only. Empirical relations have high statistical significance and properties of core samples lie within 90% prediction bands of developed regression functions of quarry samples. With triaxial tests we determined linearized Mohr-Coulomb failure criteria, expressed in both principal stresses and shear and normal stresses, for quarry samples. Comparison with

  19. From Sand to Rock: a teaching activity to introduce beach dynamics.

    NASA Astrophysics Data System (ADS)

    Gravina, Teresita

    2015-04-01

    The Italian coastline is about 7,500 km long; approximately 53% of the coastlines are low or deltaic coastlines, while 3,240 km were mainly composed of sand or gravel beaches. Most of the Italian coastal environment suffers from intense and growing urbanization, tourism and industry pressure, which could partly explain that 42% of Italian beaches experience erosion. Terracina is situated Lazio (Central Italy), a region strongly impacted by coastal erosion, and for this reason we organized a teaching activity, carried out with fourth year high school classes, in order to help students to understand sand beach dynamics, acquisition of geology issues and land conservation and preservation skills. We decided to focus our activity on the mineralogical composition of beach sand in order to relate beach formations with the geological evolution of the territory. Sand beach minerals were used as tracers in order to support students to understand dynamics that influence beach formations. In addition to mineral characteristic recognition, this activity allows us to introduce the beach balance concept and the phenomena that regulate sediment balance, in order to allow students to consider beaches as a resource which needs to be preserved. Sand mineralogical composition data is treated in a worksheet to elaborate simple statistical analysis in order to recognize the mineral composition of Terracina beach sand's rock sources. This exercise allows students to find relationships between regional geology and beach sand's composition. Finally, statistical evidence could be compared with geological maps of the area in order to find the probable provenance of sand's rock source and rocks recognition thanks to related morphologies. Our main purpose was to help students to understand that beaches are dynamic systems subject to anthropogenic pressure and for this reason they needed to be preserved. Proposed teaching activities involve topics related to students' living territory and to

  20. Assessment of rock mechanical behavior considering stress dependent stiffness of the cracked domain within crack tensor-based approach

    NASA Astrophysics Data System (ADS)

    Takemura, T.; Panaghi, K.; Golshani, A.; Takahashi, M.; Sato, M.

    2015-12-01

    The analyses dedicated to media with prevalent discontinuities such as rocks has mostly been limited to inevitable simplifications to make engineering judgments on the material behavior feasible. Such assumptions, though favorable in numerical simulations, usually lead to overestimations in aseismic design of earthen structures. One of the forbidding tasks in modeling rock behavior is taking the stress dependency of stiffness into consideration which implies more complicated formulations. Although the theoretical relationship for such computations has already been proposed by scholars, there still remains some gaps in the real-world application of the aforementioned. The crack tensor-based formulation in describing stress-strain behavior of cracked rock is a case in point in which the fourth-rank crack tensor effect is usually ignored due to adopting equal normal and shear stiffnesses for the medium. Once the stiffnesses were distinguished in different values, the accompanying condition imposed by the formulation requires computation of fourth rank tensor which has not been obtained in a practical manner so far. In the present study, we aim to acquire the values via experimental measurements and implement the results to further improve the accuracy of the formulation used in characterizing mechanical behavior of rock samples.

  1. [Molecular mechanisms regulating the activity of macrophages].

    PubMed

    Onoprienko, L V

    2011-01-01

    This article reviews modern concepts of the most common types of macrophage activation: classical, alternative, and type II. Molecular mechanisms of induction and regulation of these three types of activation are discussed. Any population of macrophages was shown to change its properties depending on its microenvironment and concrete biological situation (the "functional plasticity of macrophages"). Many intermediate states of macrophages were described along with the most pronounced and well-known activation types (classical activation, alternative activation, and type II activation). These intermediate states are characterized by a variety of combinations of their biological properties, including elements of the three afore mentioned types of activation. Macrophage activity is regulated by a complex network of interrelated cascade mechanisms.

  2. Bibliography for acid-rock drainage and selected acid-mine drainage issues related to acid-rock drainage from transportation activities

    USGS Publications Warehouse

    Bradley, Michael W.; Worland, Scott C.

    2015-01-01

    Acid-rock drainage occurs through the interaction of rainfall on pyrite-bearing formations. When pyrite (FeS2) is exposed to oxygen and water in mine workings or roadcuts, the mineral decomposes and sulfur may react to form sulfuric acid, which often results in environmental problems and potential damage to the transportation infrastructure. The accelerated oxidation of pyrite and other sulfidic minerals generates low pH water with potentially high concentrations of trace metals. Much attention has been given to contamination arising from acid mine drainage, but studies related to acid-rock drainage from road construction are relatively limited. The U.S. Geological Survey, in cooperation with the Tennessee Department of Transportation, is conducting an investigation to evaluate the occurrence and processes controlling acid-rock drainage and contaminant transport from roadcuts in Tennessee. The basic components of acid-rock drainage resulting from transportation activities are described and a bibliography, organized by relevant categories (remediation, geochemical, microbial, biological impact, and secondary mineralization) is presented.

  3. RhoA activation promotes transendothelial migration of monocytes via ROCK.

    PubMed

    Honing, Henk; van den Berg, Timo K; van der Pol, Susanne M A; Dijkstra, Christine D; van der Kammen, Rob A; Collard, John G; de Vries, Helga E

    2004-03-01

    Monocyte infiltration into inflamed tissue requires the initial arrest of the cells on the endothelium followed by firm adhesion and their subsequent migration. Migration of monocytes and other leukocytes is believed to involve a coordinated remodeling of the actin cytoskeleton. The small GTPases RhoA, Rac1, and Cdc42 are critical regulators of actin reorganization. In this study, we have investigated the role of Rho-like GTPases RhoA, Rac1, and Cdc42 in the adhesion and migration of monocytes across brain endothelial cells by expressing their constitutively active or dominant-negative constructs in NR8383 rat monocytic cells. Monocytes expressing the active form of Cdc42 show a reduced migration, whereas Rac1 expression did not affect adhesion or migration. In contrast, expression of the active form of RhoA in monocytes leads to a dramatic increase in their adhesion and migration across endothelial cells. The effect of RhoA was found to be mediated by its down-stream effector Rho kinase (ROCK), as pretreatment with the selective ROCK inhibitor Y-27632 prevented this enhanced adhesion and migration. These results demonstrate that RhoA activation in monocytes is sufficient to enhance adhesion and migration across monolayers of endothelial cells. PMID:14634067

  4. RhoA activation promotes transendothelial migration of monocytes via ROCK.

    PubMed

    Honing, Henk; van den Berg, Timo K; van der Pol, Susanne M A; Dijkstra, Christine D; van der Kammen, Rob A; Collard, John G; de Vries, Helga E

    2004-03-01

    Monocyte infiltration into inflamed tissue requires the initial arrest of the cells on the endothelium followed by firm adhesion and their subsequent migration. Migration of monocytes and other leukocytes is believed to involve a coordinated remodeling of the actin cytoskeleton. The small GTPases RhoA, Rac1, and Cdc42 are critical regulators of actin reorganization. In this study, we have investigated the role of Rho-like GTPases RhoA, Rac1, and Cdc42 in the adhesion and migration of monocytes across brain endothelial cells by expressing their constitutively active or dominant-negative constructs in NR8383 rat monocytic cells. Monocytes expressing the active form of Cdc42 show a reduced migration, whereas Rac1 expression did not affect adhesion or migration. In contrast, expression of the active form of RhoA in monocytes leads to a dramatic increase in their adhesion and migration across endothelial cells. The effect of RhoA was found to be mediated by its down-stream effector Rho kinase (ROCK), as pretreatment with the selective ROCK inhibitor Y-27632 prevented this enhanced adhesion and migration. These results demonstrate that RhoA activation in monocytes is sufficient to enhance adhesion and migration across monolayers of endothelial cells.

  5. Mechanism for the activation of glutamate receptors

    Cancer.gov

    Scientists at the NIH have used a technique called cryo-electron microscopy to determine a molecular mechanism for the activation and desensitization of ionotropic glutamate receptors, a prominent class of neurotransmitter receptors in the brain and spina

  6. ACOUSTICAL IMAGING AND MECHANICAL PROPERTIES OF SOFT ROCK AND MARINE SEDIMENTS

    SciTech Connect

    Thurman E. Scott, Jr., Ph.D.; Younane Abousleiman, Ph.D.; Musharraf Zaman, Ph.D., P.E.

    2002-04-30

    } and {alpha}{sub h}, using the equations of Abousleiman et al. (1996). A series of experiments have been conducted, on an initially inherently isotropic Berea sandstone rock sample, to dynamically determine these anisotropic Biot's parameters during deformational pathway experiments. Data acquired during hydrostatic, triaxial, and uniaxial strain pathway experiments indicates that Biot's effective stress parameter changes significantly if the applied stresses are not hydrostatic. Variations, as large as 20% between the axial (vertical) and lateral (horizontal) Biot's effective stress parameters, were observed in some experiments. A series of triaxial compression experiments have been conducted on unconsolidated sand (Oil Creek sand) to determine the pressure/stress conditions which would be favorable for liquefaction. Liquefaction of geopressured sands is thought to be one of the major causative mechanisms of damaging shallow water flows. The experiments were developed to determine if: (1) liquefaction could be made to occur in this particular sand at high confining pressures, and (2) the state of liquefication had the same nature at high pressure conditions typical of shallow water flows as it does in low confining pressure soil mechanics tests. A series of undrained triaxial experiments were successfully used to document that the Oil Creek sand could undergo liquefaction. The nature (i.e., the shape of the deformational pathway in mean pressure/shear stress space) was very similar to those observed in soil mechanics experiments. The undrained triaxial experiments also indicated that this sand would strain soften at relatively high confining pressures--a necessary precursor to liquefaction. These experiments serve as a starting point for a series of acoustic experiments to determine the signature of compressional and shear wave properties as the sand packs approach the state of liquefaction (and shallow water flows).

  7. Anti-cancer effect of ursolic acid activates apoptosis through ROCK/PTEN mediated mitochondrial translocation of cofilin-1 in prostate cancer

    PubMed Central

    Gai, Wen-Tao; Yu, Da-Peng; Wang, Xin-Sheng; Wang, Pei-Tao

    2016-01-01

    Ursolic acid is a type of pentacyclic triterpene compound with multiple pharmacological activities including cancer resistance, protection from liver injury, antisepsis, anti-inflammation and antiviral activity. The present study aimed to investigate the anticancer effect of ursolic acid. Ursolic acid activates cell apoptosis and its pro-apoptotic mechanism remains to be fully elucidated. Cell Counting kit-8 assays, flow cytometric analysis and analysis of caspase-3 and caspase-9 activity were used to estimate the anticancer effect of ursolic acid on DU145 prostate cancer cells. The protein expression of cytochrome c, rho-associated protein kinase (ROCK), phosphatase and tensin homolog (PTEN) and cofilin-1 were examined using western blot analysis. In the present study, ursolic acid significantly suppressed cell growth and induced apoptosis, as well as increasing caspase-3 and caspase-9 activities of DU145 cells. Furthermore, cytoplasmic and mitochondrial cytochrome c protein expression was significantly activated and suppressed, respectively, by ursolic acid. Ursolic acid significantly suppressed the ROCK/PTEN signaling pathway and inhibited cofilin-1 protein expression in DU145 cells. The results of the present study indicate that the anticancer effect of ursolic acid activates cell apoptosis through ROCK/PTEN mediated mitochondrial translocation of cofilin-1 in prostate cancer.

  8. Anti-cancer effect of ursolic acid activates apoptosis through ROCK/PTEN mediated mitochondrial translocation of cofilin-1 in prostate cancer

    PubMed Central

    Gai, Wen-Tao; Yu, Da-Peng; Wang, Xin-Sheng; Wang, Pei-Tao

    2016-01-01

    Ursolic acid is a type of pentacyclic triterpene compound with multiple pharmacological activities including cancer resistance, protection from liver injury, antisepsis, anti-inflammation and antiviral activity. The present study aimed to investigate the anticancer effect of ursolic acid. Ursolic acid activates cell apoptosis and its pro-apoptotic mechanism remains to be fully elucidated. Cell Counting kit-8 assays, flow cytometric analysis and analysis of caspase-3 and caspase-9 activity were used to estimate the anticancer effect of ursolic acid on DU145 prostate cancer cells. The protein expression of cytochrome c, rho-associated protein kinase (ROCK), phosphatase and tensin homolog (PTEN) and cofilin-1 were examined using western blot analysis. In the present study, ursolic acid significantly suppressed cell growth and induced apoptosis, as well as increasing caspase-3 and caspase-9 activities of DU145 cells. Furthermore, cytoplasmic and mitochondrial cytochrome c protein expression was significantly activated and suppressed, respectively, by ursolic acid. Ursolic acid significantly suppressed the ROCK/PTEN signaling pathway and inhibited cofilin-1 protein expression in DU145 cells. The results of the present study indicate that the anticancer effect of ursolic acid activates cell apoptosis through ROCK/PTEN mediated mitochondrial translocation of cofilin-1 in prostate cancer. PMID:27698874

  9. Nanoscale Properties of Rocks and Subduction Zone Rheology: Inferences for the Mechanisms of Deep Earthquakes

    NASA Astrophysics Data System (ADS)

    Riedel, M. R.

    2007-12-01

    Grain boundaries are the key for the understanding of mineral reaction kinetics. More generally, nanometer scale processes involved in breaking and establishing bonds at reaction sites determine how and at which rate bulk rock properties change in response to external tectonic forcing and possibly feed back into various geodynamic processes. A particular problem is the effects of grain-boundary energy on the kinetics of the olivine-spinel phase transformation in subducting slabs. Slab rheology is affected in many ways by this (metastable) mineral phase change. Sluggish kinetics due to metastable hindrance is likely to cause particular difficulties, because of possible strong non-linear feedback loops between strain-rate and change of creep properties during transformation. In order to get these nanoscale properties included into thermo-mechanical models, reliable kinetic data is required. The measurement of grain-boundary energies is, however, a rather difficult problem. Conventional methods of grain boundary surface tension measurement include (a) equilibrium angles at triple junction (b) rotating ball method (c) thermal groove method, and others (Gottstein & Shvindlerman, 1999). Here I suggest a new method that allows for the derivation of grain-boundary energies for an isochemical phase transformation based on experimental (in-situ) kinetic data in combination with a corresponding dynamic scaling law (Riedel and Karato, 1997). The application of this method to the olivine-spinel phase transformation in subducting slabs provides a solution to the extrapolation problem of measured kinetic data: Any kinetic phase boundary measured at the laboratory time scale can be "scaled" to the correct critical isotherm at subduction zones, under experimentelly "forbidden" conditions (Liou et al., 2000). Consequences for the metastability hypothesis that relates deep seismicity with olivine metastability are derived and discussed. References: Gottstein G, Shvindlerman LS (1999

  10. Heterogeneity in friction strength of an active fault by incorporation of fragments of the surrounding host rock

    NASA Astrophysics Data System (ADS)

    Kato, Naoki; Hirono, Tetsuro

    2016-07-01

    To understand the correlation between the mesoscale structure and the frictional strength of an active fault, we performed a field investigation of the Atera fault at Tase, central Japan, and made laboratory-based determinations of its mineral assemblages and friction coefficients. The fault zone contains a light gray fault gouge, a brown fault gouge, and a black fault breccia. Samples of the two gouges contained large amounts of clay minerals such as smectite and had low friction coefficients of approximately 0.2-0.4 under the condition of 0.01 m s-1 slip velocity and 0.5-2.5 MP confining pressure, whereas the breccia contained large amounts of angular quartz and feldspar and had a friction coefficient of 0.7 under the same condition. Because the fault breccia closely resembles the granitic rock of the hangingwall in composition, texture, and friction coefficient, we interpret the breccia as having originated from this protolith. If the mechanical incorporation of wall rocks of high friction coefficient into fault zones is widespread at the mesoscale, it causes the heterogeneity in friction strength of fault zones and might contribute to the evolution of fault-zone architectures.

  11. Conical Euler simulation and active suppression of delta wing rocking motion

    NASA Technical Reports Server (NTRS)

    Lee, Elizabeth M.; Batina, John T.

    1990-01-01

    A conical Euler code was developed to study unsteady vortex-dominated flows about rolling highly-swept delta wings, undergoing either forced or free-to-roll motions including active roll suppression. The flow solver of the code involves a multistage Runge-Kutta time-stepping scheme which uses a finite volume spatial discretization of the Euler equations on an unstructured grid of triangles. The code allows for the additional analysis of the free-to-roll case, by including the rigid-body equation of motion for its simultaneous time integration with the governing flow equations. Results are presented for a 75 deg swept sharp leading edge delta wing at a freestream Mach number of 1.2 and at alpha equal to 10 and 30 deg angle of attack. A forced harmonic analysis indicates that the rolling moment coefficient provides: (1) a positive damping at the lower angle of attack equal to 10 deg, which is verified in a free-to-roll calculation; (2) a negative damping at the higher angle of attack equal to 30 deg at the small roll amplitudes. A free-to-roll calculation for the latter case produces an initially divergent response, but as the amplitude of motion grows with time, the response transitions to a wing-rock type of limit cycle oscillation. The wing rocking motion may be actively suppressed, however, through the use of a rate-feedback control law and antisymmetrically deflected leading edge flaps. The descriptions of the conical Euler flow solver and the free-to-roll analysis are presented. Results are also presented which give insight into the flow physics associated with unsteady vortical flows about forced and free-to-roll delta wings, including the active roll suppression of this wing-rock phenomenon.

  12. SDF-1α stiffens myeloma bone marrow mesenchymal stromal cells through the activation of RhoA-ROCK-Myosin II

    PubMed Central

    Choi, Dong Soon; Stark, Daniel J.; Raphael, Robert M.; Wen, Jianguo; Su, Jing; Zhou, Xiaobo; Chang, Chung-Che; Zu, Youli

    2015-01-01

    Multiple myeloma (MM) is a B lymphocyte malignancy that remains incurable despite extensive research efforts. This is due, in part, to frequent disease recurrences associated with the persistence of myeloma cancer stem cells (mCSCs). Bone marrow mesenchymal stromal cells (BMSCs) play critical roles in supporting mCSCs through genetic or biochemical alterations. Previously, we identified mechanical distinctions between BMSCs isolated from MM patients (mBMSCs) and those present in the BM of healthy individuals (nBMSCs). These properties of mBMSC contributed to their ability to preferentially support mCSCs. To further illustrate mechanisms underlying the differences between mBMSCs and nBMSCs, here we report that (i) mBMSCs express an abnormal, constitutively high level of phosphorylated Myosin II, which leads to stiffer membrane mechanics, (ii) mBMSCs are more sensitive to SDF-1α-induced activation of MYL2 through the G(i./o)-PI3K-RhoA-ROCK-Myosin II signaling pathway, affecting Young’s modulus in BMSCs and (iii) activated Myosin II confers increased cell contractile potential, leading to enhanced collagen matrix remodeling and promoting the cell–cell interaction between mCSCs and mBMSCs. Together, our findings suggest that interfering with SDF-1α signaling may serve as a new therapeutic approach for eliminating mCSCs by disrupting their interaction with mBMSCs. PMID:25137150

  13. Observations, models, and mechanisms of failure of surface rocks surrounding planetary surface loads

    NASA Technical Reports Server (NTRS)

    Schultz, R. A.; Zuber, M. T.

    1994-01-01

    Geophysical models of flexural stresses in an elastic lithosphere due to an axisymmetric surface load typically predict a transition with increased distance from the center of the load of radial thrust faults to strike-slip faults to concentric normal faults. These model predictions are in conflict with the absence of annular zones of strike-slip faults around prominent loads such as lunar maria, Martian volcanoes, and the Martian Tharsis rise. We suggest that this paradox arises from difficulties in relating failure criteria for brittle rocks to the stress models. Indications that model stresses are inappropriate for use in fault-type prediction include (1) tensile principal stresses larger than realistic values of rock tensile strength, and/or (2) stress differences significantly larger than those allowed by rock-strength criteria. Predictions of surface faulting that are consistent with observations can be obtained instead by using tensile and shear failure criteria, along with calculated stress differences and trajectories, with model stress states not greatly in excess of the maximum allowed by rock fracture criteria.

  14. Mechanical Behavior of Brittle Rock-Like Specimens with Pre-existing Fissures Under Uniaxial Loading: Experimental Studies and Particle Mechanics Approach

    NASA Astrophysics Data System (ADS)

    Cao, Ri-hong; Cao, Ping; Lin, Hang; Pu, Cheng-zhi; Ou, Ke

    2016-03-01

    Joints and fissures with similar orientation or characteristics are common in natural rocks; the inclination and density of the fissures affect the mechanical properties and failure mechanism of the rock mass. However, the strength, crack coalescence pattern, and failure mode of rock specimens containing multi-fissures have not been studied comprehensively. In this paper, combining similar material testing and discrete element numerical method (PFC2D), the peak strength and failure characteristics of rock-like materials with multi-fissures are explored. Rock-like specimens were made of cement and sand and pre-existing fissures created by inserting steel shims into cement mortar paste and removing them during curing. The peak strength of multi-fissure specimens depends on the fissure angle α (which is measured counterclockwise from horizontal) and fissure number ( N f). Under uniaxial compressional loading, the peak strength increased with increasing α. The material strength was lowest for α = 25°, and highest for α = 90°. The influence of N f on the peak strength depended on α. For α = 25° and 45°, N f had a strong effect on the peak strength, while for higher α values, especially for the 90° sample, there were no obvious changes in peak strength with different N f. Under uniaxial compression, the coalescence modes between the fissures can be classified into three categories: S-mode, T-mode, and M-mode. Moreover, the failure mode can be classified into four categories: mixed failure, shear failure, stepped path failure, and intact failure. The failure mode of the specimen depends on α and N f. The peak strength and failure modes in the numerically simulated and experimental results are in good agreement.

  15. Rho-associated kinase (ROCK) inhibition reverses low cell activity on hydrophobic surfaces

    SciTech Connect

    Tian, Yu Shun; Kim, Hyun Jung; Kim, Hyun-Man

    2009-08-28

    Hydrophobic polymers do not offer an adequate scaffold surface for cells to attach, migrate, proliferate, and differentiate. Thus, hydrophobic scaffolds for tissue engineering have traditionally been physicochemically modified to enhance cellular activity. However, modifying the surface by chemical or physical treatment requires supplementary engineering procedures. In the present study, regulation of a cell signal transduction pathway reversed the low cellular activity on a hydrophobic surface without surface modification. Inhibition of Rho-associated kinase (ROCK) by Y-27632 markedly enhanced adhesion, migration, and proliferation of osteoblastic cells cultured on a hydrophobic polystyrene surface. ROCK inhibition regulated cell-cycle-related molecules on the hydrophobic surface. This inhibition also decreased expression of the inhibitors of cyclin-dependent kinases such as p21{sup cip1} and p27{sup kip1} and increased expression of cyclin A and D. These results indicate that defective cellular activity on the hydrophobic surface can be reversed by the control of a cell signal transduction pathway without physicochemical surface modification.

  16. Rho-associated kinase (ROCK) inhibition reverses low cell activity on hydrophobic surfaces.

    PubMed

    Tian, Yu Shun; Kim, Hyun Jung; Kim, Hyun-Man

    2009-08-28

    Hydrophobic polymers do not offer an adequate scaffold surface for cells to attach, migrate, proliferate, and differentiate. Thus, hydrophobic scaffolds for tissue engineering have traditionally been physicochemically modified to enhance cellular activity. However, modifying the surface by chemical or physical treatment requires supplementary engineering procedures. In the present study, regulation of a cell signal transduction pathway reversed the low cellular activity on a hydrophobic surface without surface modification. Inhibition of Rho-associated kinase (ROCK) by Y-27632 markedly enhanced adhesion, migration, and proliferation of osteoblastic cells cultured on a hydrophobic polystyrene surface. ROCK inhibition regulated cell-cycle-related molecules on the hydrophobic surface. This inhibition also decreased expression of the inhibitors of cyclin-dependent kinases such as p21(cip1) and p27(kip1) and increased expression of cyclin A and D. These results indicate that defective cellular activity on the hydrophobic surface can be reversed by the control of a cell signal transduction pathway without physicochemical surface modification.

  17. Mechanisms of Nutrient Acquisition by Rock Eating Microbes Revealed by Proteomics

    NASA Astrophysics Data System (ADS)

    Bryce, C. C.; Martin, S.; LeBihan, T.; Cockell, C.

    2013-12-01

    In nutrient poor terrestrial environments such as fresh lava flows, bioessential elements contained within surrounding rocks can be an important source of nutrients for the microbial community. The role of microbes in the alteration of rock surfaces, driven by this nutrient requirement, is widely accepted and is known to play an important role in CO2 drawdown as well as influencing nutrient flux to the biosphere. There is, however, limited knowledge of the biological processes which facilitate the uptake of bioessential elements from rocks. Using a technique known as 'shotgun' proteomics we have investigated the cellular processes involved in the uptake of iron, calcium and magnesium from fresh basalt in the heavy metal resistant bacterium Cupriavidus metallidurans CH34. Quantitative proteomics allows us to obtain a detailed snapshot of the protein complement of cells. By comparing cultures grown under normal growth conditions to cultures grown with basalt as an alternative iron, calcium or magnesium source, we can highlight proteins which are differentially expressed and therefore important for life in a rocky environment. We observe that the use of rock-bound nutrients induces a complex metabolic response in C.metallidurans which is distinct from the effects observed in the presence of rocks in normal growth medium. This is evidenced, for example, by the upregulation of a number of proteins involved in alternative energy-producing processes such as chemolithotrophy, sulphur oxidation and hydrogen oxidation compared to control cultures. This work has implications for the understanding of how microbes forge a life for themselves from the Earth's crust and highlights the importance of the field of proteomics for the study of life in terrestrial environments.

  18. Mechanical Aqueous Alteration Dominates Textures of Gale Crater Rocks: Mars Hand Lens Imager (MAHLI) Results

    NASA Astrophysics Data System (ADS)

    Aileen Yingst, R.; Minitti, Michelle; Edgett, Kenneth; McBride, Marie; Stack, Kathryn

    2015-04-01

    The Mars Hand Lens Imager (MAHLI) acquired sub-mm/pixel scale color images of over 70 individual rocks and outcrops during Curiosity's first year on Mars, permitting the study of textures down to the distinction between silt and very fine sand. We group imaged rock textures into classes based on their grain size, sorting, matrix characteristics, and abundance of pores. Because the recent campaign at Pahrump Hills acquired many more MAHLI images than elsewhere along the rover traverse [6], textural analysis there is more detailed and thus types observed there are sub-divided. Mudstones: These rocks contain framework grains smaller than the highest resolution MAHLI images (16 μm/pixel), and thus are interpreted to consist of grains that are silt-sized or smaller. Some rocks contain nodules, sulfate veins, and Mg-enriched erosionally-resistant ridges. The Pahrump Hills region contains mudstones of at least four different sub-textures: recessive massive, recessive parallel-laminated, resistant laminated-to-massive, and resistant cross-stratified. Recessive mudstones are slope-forming; parallel-laminated recessive mudstones display mm-scale parallel (and in some cases rhythmic) lamination that extends laterally for many meters, and are interbedded with recessive massive mudstones. Coarse cm- to mm-scale laminae appear within resistant mudstones though some portions are more massive; laminae tend to be traceable for cm to meters. Well-sorted sandstones: Rocks in this class are made of gray, fine-to-medium sand and exhibit little to no porosity. Two examples of this class show fine lineations with sub-mm spacing. Aillik, a target in the Shaler outcrop, shows abundant cross-lamination. The Pahrump Hills region contains a sub-texture of well-sorted, very fine to fine-grained cross-stratified sandstone at the dune and ripple-scale. Poorly-sorted sandstones. This class is subdivided into two sub-classes: rounded, coarse-to-very coarse sand grains of variable colors and

  19. Potential fluid mechanic pathways of platelet activation

    PubMed Central

    Shadden, Shawn C.; Hendabadi, Sahar

    2012-01-01

    Platelet activation is a precursor for blood clotting, which plays leading roles in many vascular complications and causes of death. Platelets can be activated by chemical or mechanical stimuli. Mechanically, platelet activation has been shown to be a function of elevated shear stress and exposure time. These contributions can be combined by considering the cumulative stress or strain on a platelet as it is transported. Here we develop a framework for computing a hemodynamic-based activation potential that is derived from a Lagrangian integral of strain rate magnitude. We demonstrate that such a measure is generally maximized along, and near to, distinguished material surfaces in the flow. The connections between activation potential and these structures are illustrated through stenotic flow computations. We uncover two distinct structures that may explain observed thrombus formation at the apex and downstream of stenoses. More broadly, these findings suggest fundamental relationships may exist between potential fluid mechanic pathways for mechanical platelet activation and the mechanisms governing their transport. PMID:22782543

  20. The Cretaceous-Tertiary extinction: A lethal mechanism involving anhydrite target rocks

    USGS Publications Warehouse

    Brett, R.

    1992-01-01

    The Chicxulub Crater, Yucatan, Mexico, is a leading contender as the site for the impact event that caused the Cretaceous-Tertiary (K-T) extinctions. A considerable thickness of anhydrite (CaSO4) forms part of the target rock. High temperatures resulting from impact would drive SO2 off from the anhydrite. Hundreds of billions of tonnes of sulfuric acid aerosol would thus enter the stratosphere and cause considerable cooling of the Earth's surface, decrease photosynthesis by orders of magnitude, deplete the ozone layer, and permit increased UV radiation to reach the Earth's surface. Finally, the aerosol would fall back to Earth as acid rain and devastate land and some lacustrine biota and near-surface marine creatures. The presence of anhydrite in the Chicxulub target rock may thus help explain the many extinctions observed at the K-T boundary. ?? 1992.

  1. Dissecting Oceanic Detachment Faults: Fault Zone Geometry, Deformation Mechanisms, and Nature of Fluid-Rock Interactions

    NASA Astrophysics Data System (ADS)

    Bonnemains, D.; Escartin, J.; Verlaguet, A.; Andreani, M.; Mevel, C.

    2015-12-01

    To understand the extreme strain localization at long-lived oceanic detachment faults rooting deeply below the axis, we present results of geological investigations at the 13°19'N detachment along the Mid-Atlantic Ridge, conducted during the ODEMAR cruise (Nov-Dec13, NO Pourquoi Pas?) with ROV Victor6000 (IFREMER). During this cruise we investigated and sampled the corrugated fault to understand its geometry, nature of deformation, and links to fluid flow. We identified and explored 7 fault outcrops on the flanks of microbathymetric striations subparallel to extension. These outcrops expose extensive fault planes, with the most prominent ones extending 40-90m laterally, and up to 10 m vertically. These fault surfaces systematically show subhorizontal striations subparallel to extension, and define slabs of fault-rock that are flat and also striated at sample scale. Visual observations show a complex detachment fault zone, with anastomosing fault planes at outcrop scale (1-10 m), with a highly heterogeneous distribution of deformation. We observe heterogeneity in fault-rock nature at outcrop scale. In situ samples from striated faults are primarily basalt breccias with prior green-schist facies alteration, and a few ultramafic fault-rocks that show a complex deformation history, with early schistose textures, brittlely reworked as clasts within the fault. The basalt breccias show variable silicification and associated sulfides, recording important fluid-rock interactions during exhumation. To understand the link between fluid and deformation during exhumation, we will present microstructural observation of deformation textures, composition, and distribution and origin of quartz and sulfides, as well as constraints on the temperature of silicifying fluids from fluid inclusions in quartz. These results allow us to characterize in detail the detachment fault zone geometry, and investigate the timing of silicification relative to deformation.

  2. Influence of mechanical rock properties and fracture healing rate on crustal fluid flow dynamics

    NASA Astrophysics Data System (ADS)

    Sachau, Till; Bons, Paul; Gomez-Rivas, Enrique; Koehn, Daniel; de Riese, Tamara

    2016-04-01

    Fluid flow in the Earth's crust is very slow over extended periods of time, during which it occurs within the connected pore space of rocks. If the fluid production rate exceeds a certain threshold, matrix permeability alone is insufficient to drain the fluid volume and fluid pressure builds up, thereby reducing the effective stress supported by the rock matrix. Hydraulic fractures form once the effective pressure exceeds the tensile strength of the rock matrix and act subsequently as highly effective fluid conduits. Once local fluid pressure is sufficiently low again, flow ceases and fractures begin to heal. Since fluid flow is controlled by the alternation of fracture permeability and matrix permeability, the flow rate in the system is strongly discontinuous and occurs in intermittent pulses. Resulting hydraulic fracture networks are largely self-organized: opening and subsequent healing of hydraulic fractures depends on the local fluid pressure and on the time-span between fluid pulses. We simulate this process with a computer model and describe the resulting dynamics statistically. Special interest is given to a) the spatially and temporally discontinuous formation and closure of fractures and fracture networks and b) the total flow rate over time. The computer model consists of a crustal-scale dual-porosity setup. Control parameters are the pressure- and time-dependent fracture healing rate, and the strength and the permeability of the intact rock. Statistical analysis involves determination of the multifractal properties and of the power spectral density of the temporal development of the total drainage rate and hydraulic fractures. References Bons, P. D. (2001). The formation of large quartz veins by rapid ascent of fluids in mobile hydrofractures. Tectonophysics, 336, 1-17. Miller, S. a., & Nur, A. (2000). Permeability as a toggle switch in fluid-controlled crustal processes. Earth and Planetary Science Letters, 183(1-2), 133-146. Sachau, T., Bons, P. D

  3. On Discrimination of Thermal Versus Mechanical Effects of Shock on Rock Magnetic Properties of Spherically Shocked up to ˜10-140 GPa Basalt and Diabase

    NASA Astrophysics Data System (ADS)

    Bezaeva, N. S.; Swanson-Hysell, N. L.; Tikoo, S. M.; Kars, M.; Egli, R.

    2016-08-01

    We present a new experimental method of discrimination in shock-recovery experiments between thermal and mechanical effects of shock on rock magnetic properties of spherically shocked (Ti) magnetite-bearing basalt and diabase.

  4. On Discrimination of Thermal Versus Mechanical Effects of Shock on Rock Magnetic Properties of Spherically Shocked up to ˜10–140 GPa Basalt and Diabase

    NASA Astrophysics Data System (ADS)

    Bezaeva, N. S.; Swanson-Hysell, N. L.; Tikoo, S. M.; Kars, M.; Egli, R.

    2016-08-01

    We present a new experimental method of discrimination in shock-recovery experiments between thermal and mechanical effects of shock on rock magnetic properties of spherically shocked (Ti) magnetite-bearing basalt and diabase.

  5. Spironolactone Lowers Portal Hypertension by Inhibiting Liver Fibrosis, ROCK-2 Activity and Activating NO/PKG Pathway in the Bile-Duct-Ligated Rat

    PubMed Central

    Ji, Hong-Li; Pan, Chun-Qiu; Huang, Shan; Yu, Chang-Hui; Xiao, Li-Ming; Cui, Kai; Ni, Shu-Yuan; Zhang, Zhen-Shu; Li, Xu

    2012-01-01

    Objective Aldosterone, one of the main peptides in renin angiotensin aldosterone system (RAAS), has been suggested to mediate liver fibrosis and portal hypertension. Spironolactone, an aldosterone antagonist, has beneficial effect on hyperdynamic circulation in clinical practice. However, the mechanisms remain unclear. The present study aimed to investigate the role of spionolactone on liver cirrhosis and portal hypertension. Methods Liver cirrhosis was induced by bile duct ligation (BDL). Spironolactone was administered orally (20 mg/kg/d) after bile duct ligation was performed. Liver fibrosis was assessed by histology, Masson's trichrome staining, and the measurement of hydroxyproline and type I collagen content. The activation of HSC was determined by analysis of alpha smooth muscle actin (α-SMA) expression. Protein expressions and protein phosphorylation were determined by immunohistochemical staining and Western blot analysis, Messenger RNA levels by quantitative real time polymerase chain reaction (Q-PCR). Portal pressure and intrahepatic resistance were examined in vivo. Results Treatment with spironolactone significantly lowered portal pressure. This was associated with attenuation of liver fibrosis, intrahepatic resistance and inhibition of HSC activation. In BDL rat liver, spironolactone suppressed up-regulation of proinflammatory cytokines (TNFα and IL-6). Additionally, spironolactone significantly decreased ROCK-2 activity without affecting expression of RhoA and Ras. Moreover, spironolactone markedly increased the levels of endothelial nitric oxide synthase (eNOS), phosphorylated eNOS and the activity of NO effector- protein kinase G (PKG) in the liver. Conclusion Spironolactone lowers portal hypertension by improvement of liver fibrosis and inhibition of intrahepatic vasoconstriction via down-regulating ROCK-2 activity and activating NO/PKG pathway. Thus, early spironolactone therapy might be the optional therapy in cirrhosis and portal hypertension

  6. Rocks and Minerals.

    ERIC Educational Resources Information Center

    Naturescope, 1987

    1987-01-01

    Provides background information on rocks and minerals, including the unique characteristics of each. Teaching activities on rock-hunting and identification, mineral configurations, mystery minerals, and growing crystals are provided. Reproducible worksheets are included for two of the activities. (TW)

  7. Experimental Investigation on the Influence of High Pressure and High Temperature on the Mechanical Properties of Deep Reservoir Rocks

    NASA Astrophysics Data System (ADS)

    Zhang, Peng; Mishra, Brijes; Heasley, Keith A.

    2015-11-01

    Deep and ultra-deep resources extraction has resulted in the challenge of drilling into high-pressure, high-temperature (HPHT) environments. Drilling challenges at such extreme conditions prompted NETL to develop a specialized ultra-deep drilling simulator (UDS) for investigating drill behavior in such conditions. Using the UDS apparatus, complex laboratory tests were performed on Carthage marble (Warsaw limestone) and Crab Orchard sandstone, which represent the rocks in the basins of the Tuscaloosa trend in southern Louisiana and the Arbuckle play in Oklahoma and North Texas. Additionally, numerical models of the UDS were developed for performing parametric analyses that would be impossible with the UDS alone. Subsequently, it was found that the input properties for these two rock types at such extreme pressure and temperature conditions were unavailable. Therefore, a suite of unconfined compressive strength, indirect tensile strength, and triaxial compression tests ( σ 1 > σ 2 = σ 3) were performed on Carthage marble and Crab Orchard sandstone for investigating their behavior in HPHT environments. The HPHT experiments were performed at confining pressures ranging from atmospheric to 200 MPa, and with temperatures ranging from 25 to 180 °C. The influences of confining pressure and temperature on the mechanical properties of two rocks were investigated.

  8. Feldspathic Rock Spectral Detections on Mars: Geologic Context, Possible Formation Mechanisms, and the TES/Themis Perspective

    NASA Astrophysics Data System (ADS)

    Rogers, D.; Nekvasil, H.

    2014-12-01

    Spectral detections from VNIR imaging spectrometers OMEGA and CRISM suggest feldspar-bearing rocks with <5% mafic minerals in restricted locations on Mars. The detections have been interpreted as anorthositic, or alternatively, felsic lithologies such as granite. The detections occur in a variety of contexts, including crater central peaks, walls, and floors, intercrater plains of Noachis Terra, and the Nili patera caldera floor. Here we focus on the Noachis Terra feldspathic rock detections, and present constraints from geologic context and complementary thermal infrared measurements. We also examine mechanisms for forming feldspar-rich lavas from crystal fractionation at the base of thick Martian crust. Noachis Terra exposures exhibit high thermal inertias and deep spectral contrast, consistent with competent, non-porous rock. They commonly overlie olivine basaltic bedrock and are ~20-25 m thick. THEMIS spectra from these units are inconsistent with quartz abundances > 5%, ruling out felsic compositions. THEMIS spectra are consistent with both anorthositic and basaltic lithologies; laboratory spectra of these lithologies are indistinguishable at THEMIS resolution. TES spectra do not match library anorthosites, with ~20-30% modeled pyroxene and ~5-10% olivine. Strong contribution from basaltic sediment to the TES spectra is unlikely given the deeper spectral contrast associated with the feldspathic units than underlying olivine basaltic bedrock. Future work will include spectral comparison with other low silica, feldspathic rocks to determine if there is an analog material that is consistent with both the VNIR and TIR observations. The geologic context of the Noachis units suggests volcanic, rather than plutonic origins, although shallow sills or subglacial eruptive units are possible. Previous experimental and modeling work by Nekvasil showed that feldspar-rich (up to 75 wt%), low-silica lavas may be produced from known Martian basalt by shallow crystallization

  9. How much does a very active rock slope contribute to the sediment budget of an alpine glacier?

    NASA Astrophysics Data System (ADS)

    Baewert, Henning; Vehling, Lucas; Glira, Philipp; Stocker-Waldhuber, Martin; Morche, David

    2014-05-01

    The ongoing glacier retreat since the mid of the 19th century has significant influence on rock slope stability in alpine high mountain areas. Due to oversteepening by glacial erosion, cold climate weathering processes and debuttressing as a consequence of stress redistribution, rock slopes adjacent to shrinking glaciers generally show an enhanced geotechnical activity. Regarding the glacier sediment budget, the rockfall material deposited on a glacier is particular important, because the debris material can be transported directly and without any intermediate storage. Therefore, gravitational mass movements contribute in a substantial way to the sediment budget of a glacier, especially as rockfall material can easily reach en- or subglacial areas through crevasses and thus affect the subglacial sediment transport and glacial erosion. Here we present the first results regarding the geotechnical rock slope activity of "Schwarze Wand". The "Schwarze Wand" is located at 2400 - 2800 m.a.s.l., right above the tongue of the Gepatschferner, which is one of the largest glaciers in Tyrol (Austria) and contemporarily affected by a high retreat rate. The rock mass consists of strong foliated paragneisses which are dissected by large joint sets. These joint sets provide sliding planes, which favor slope failures. To monitor the rock slope activity at the "Schwarze Wand", multitemporal terrestrial laser scans were carried out in 2012 and 2013 to detect and quantify mass movements. Additional, high resoluted multitemporal airborne laser scan data (10 points/m²) are available to trace larger scale rock slope deformations. The investigations are conducted by the DFG- joint research project PROSA (High-resolution measurements of morphodynamics in rapidly changing PROglacial Systems of the Alps). Our LiDAR data as well as field observations are showing enhanced rock fall activity at the scarp in the last years which is assumed to be the consequence of an activation of a larger deep

  10. Characterization of the Monument Hill fault system and implications for the active tectonics of the Red Rock Valley, Southwestern Montana

    NASA Astrophysics Data System (ADS)

    Regalla, Christine A.; Anastasio, David J.; Pazzaglia, Frank J.

    2007-08-01

    New geologic mapping, morphologic fault scarp modeling, and geomorphic metrics in the Red Rock Valley, southwestern Montana, help characterize the Quaternary history of the virtually unstudied Monument Hill fault and tectonics of the youthful and seismically active Red Rock graben. Two generations of Pleistocene surface ruptures are preserved along the Monument Hill fault. Similarity in rupture ages along multiple strands, determined from offset alluvial surfaces and morphologic modeling, suggest earthquake clusters at 22-32 ka and possibly >160 ka. Quaternary activity along the Monument Hill fault is also reflected in elongate drainage basins and channel profiles with anomalously steep reaches coincident with mapped faults. An anticlinal accommodation zone at Kidd accommodates a change in fault polarity between the en echelon Monument Hill and Red Rock faults and a northward decrease in extension within the Red Rock graben. The unique rupture histories of the Monument Hill and Red Rock faults, however, suggest the systems are not seismogenically linked and that the accommodation zone serves as a rupture barrier. The geometry, interconnectivity, and kinematics of faults in the Red Rock Valley may represent a snapshot of the early stages of extension applicable to the evolution of other Northern Basin and Range grabens.

  11. Mechanics of light-activated network polymers

    NASA Astrophysics Data System (ADS)

    Long, Kevin Nicholas

    Mechanically responsive, environmentally activated polymers can undergo large, complex deformation in response to external stimuli such as thermal, luminous, and chemical changes to the environment. Light as a stimulus provides unique application potential because it allows for remote, rapid, and isothermal activation of the material with precise spatial control via existing optical technologies. While certain systems have received considerable attention, the state of the art of most light-activated polymers is limited to basic characterization and demonstrations. To make such materials available to the engineering and scientific communities, physically based theoretical and computational tools are required to guide experimental and design efforts that capitalize on their complex photo-mechanical couplings. The central objective of this thesis is to develop a multi-physics constitutive modeling framework to simulate the continuum scale, photo mechanical behavior of light-activated polymers and implement it into a finite element analysis setting. This framework is independent of specific underlying photo-stimulation mechanisms and is discussed in the context of photo-activated shape memory polymers and network rearranging polymers. Next, the framework is applied to the light-activated network rearranging polymer system, which is relaxed of stress upon irradiation with UV light, and a suite of characterization and application oriented experiments are carried out to calibrate and validate the model's predictive capabilities. The calibrated model is used to investigate several applications such as photo-activated stress relaxation of notched specimens, bending actuation, creep, the buckling of equi-biaxially deformed and irradiated films, and photomechanically formed 1D channels and ridges. Modeling creep involves additional complexity through simultaneous deformation and irradiation, and so the model framework is extended to cover such scenarios. Experiments, finite

  12. Studies on deformation/pore pressure coupling processes at Japanese URLs and the development of ultra-high resolution FBG strain sensors for rock mechanics (Invited)

    NASA Astrophysics Data System (ADS)

    Tokunaga, T.; Matsui, H.; Zuyuan, H.; Kashiwai, Y.

    2009-12-01

    Generic, purpose-build underground research laboratories have been under construction in Japan. The objective for the construction is to conduct integrated studies on geology, geophysics, hydrogeology, rock mechanics, hydro-geochemistry, etc., to better understand the behavior and long-term stability of subsurface environments and subsurface openings. The Japan Atomic Energy Agency is responsible for the construction and selected two sites, i.e., Mizunami in central Japan and Horonobe in northern tip of Hokkaido, each representing granitic/crystalline rock environment and sedimentary formations, respectively. The construction of the Mizunami URL site began in July 2003, and the shafts reached to 400 m below ground level as of August 2009. The URL is situated in granitic rock masses including highly altered, fractured and faulted zones which are typical in Japan. The Main Shaft is situated at the fault which strikes to NNW while the Ventilation Shaft at the intact granitic rock. Sub-stages, which connect two shafts were constructed every 100 m depth interval, and several boreholes were drilled and are planned to be drilled from the shafts and the galleries for sampling rocks, groundwater, and for continuous monitoring of pore pressures. During the construction, we observed transient pore pressure responses caused by the excavation and dewatering/flooding of the shafts and boreholes. Here, we present one example of the spatio-temporal pore pressure responses caused by the rapid discharge of groundwater of which amount was 0.5 tons per minute from one pilot borehole. Pore pressure responses showed two distinct patterns, one continuous decline and the other transient increase and subsequent decline. The former pattern was observed at the locations where pore pressure transducers were set in the granitic rocks and were situated in the same block with discharge points with respect to the fault with NNW strike, while the latter in sedimentary formations and in the granite

  13. A study of the depth of weathering and its relationship to the mechanical properties of near-surface rocks in the Mojave Desert

    USGS Publications Warehouse

    Stierman, D.J.; Healy, J.H.

    1985-01-01

    Weathered granite extends 70 m deep at Hi Vista in the arid central Mojave Desert of southern California. The low strength of this granite is due to the alteration of biotite and chlorite montmorillonite. Deep weathering probably occurs in most granites, although we cannot rule out some anomalous mechanisms at Hi Vista. Geophysical instruments set in these slightly altered rocks are limited by the unstable behavior of the rocks. Thus, tectonic signals from instruments placed in shallow boreholes give vague results. Geophysical measurements of these weathered rocks resemble measurements of granitic rocks near major faults. The rheology of the rocks in which instruments are placed limits the useful sensitivity of the instruments. ?? 1985 Birkha??user Verlag.

  14. Importance of neutron energy distribution in borehole activation analysis in relatively dry, low-porosity rocks

    USGS Publications Warehouse

    Senftle, F.E.; Moxham, R.M.; Tanner, A.B.; Philbin, P.W.; Boynton, G.R.; Wager, R.E.

    1977-01-01

    To evaluate the importance of variations in the neutron energy distribution in borehole activation analysis, capture gamma-ray measurements were made in relatively dry, low-porosity gabbro of the Duluth Complex. Although sections of over a meter of solid rock were encountered in the borehole, there was significant fracturing with interstitial water leading to a substantial variation of water with depth in the borehole. The linear-correlation coefficients calculated for the peak intensities of several elements compared to the chemical core analyses were generally poor throughout the depth investigated. The data suggest and arguments are given which indicate that the variation of the thermal-to-intermediate-to-fast neutron flux density as a function of borehole depth is a serious source of error and is a major cause of the changes observed in the capture gamma-ray peak intensities. These variations in neutron energy may also cause a shift in the observed capture gamma-ray energy.

  15. Molecular mechanisms regulating NLRP3 inflammasome activation

    PubMed Central

    Jo, Eun-Kyeong; Kim, Jin Kyung; Shin, Dong-Min; Sasakawa, Chihiro

    2016-01-01

    Inflammasomes are multi-protein signaling complexes that trigger the activation of inflammatory caspases and the maturation of interleukin-1β. Among various inflammasome complexes, the NLRP3 inflammasome is best characterized and has been linked with various human autoinflammatory and autoimmune diseases. Thus, the NLRP3 inflammasome may be a promising target for anti-inflammatory therapies. In this review, we summarize the current understanding of the mechanisms by which the NLRP3 inflammasome is activated in the cytosol. We also describe the binding partners of NLRP3 inflammasome complexes activating or inhibiting the inflammasome assembly. Our knowledge of the mechanisms regulating NLRP3 inflammasome signaling and how these influence inflammatory responses offers further insight into potential therapeutic strategies to treat inflammatory diseases associated with dysregulation of the NLRP3 inflammasome. PMID:26549800

  16. Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro

    PubMed Central

    Lv, Zhimei; Hu, Mengsi; Ren, Xiaoxu; Fan, Minghua; Zhen, Junhui; Chen, Liqun; Lin, Jiangong; Ding, Nannan; Wang, Qun; Wang, Rong

    2016-01-01

    Fyn, a member of the Src family of tyrosine kinases, is a key regulator in cytoskeletal remodeling in a variety of cell types. Recent studies have demonstrated that Fyn is responsible for nephrin tyrosine phosphorylation, which will result in polymerization of actin filaments and podocyte damage. Thus detailed involvement of Fyn in podocytes is to be elucidated. In this study, we investigated the potential role of Fyn/ROCK signaling and its interactions with paxillin. Our results presented that high glucose led to filamentous actin (F-actin) rearrangement in podocytes, accompanied by paxillin phosphorylation and increased cell motility, during which Fyn and ROCK were markedly activated. Gene knockdown of Fyn by siRNA showed a reversal effect on high glucose-induced podocyte damage and ROCK activation; however, inhibition of ROCK had no significant effects on Fyn phosphorylation. These observations demonstrate that in vitro Fyn mediates high glucose-induced actin cytoskeleton remodeling of podocytes via promoting ROCK activation and paxillin phosphorylation. PMID:26881253

  17. Cell-body rocking is a dominant mechanism for flagellar synchronization in a swimming alga

    PubMed Central

    Geyer, Veikko F.; Jülicher, Frank; Howard, Jonathon; Friedrich, Benjamin M.

    2013-01-01

    The unicellular green alga Chlamydomonas swims with two flagella that can synchronize their beat. Synchronized beating is required to swim both fast and straight. A long-standing hypothesis proposes that synchronization of flagella results from hydrodynamic coupling, but the details are not understood. Here, we present realistic hydrodynamic computations and high-speed tracking experiments of swimming cells that show how a perturbation from the synchronized state causes rotational motion of the cell body. This rotation feeds back on the flagellar dynamics via hydrodynamic friction forces and rapidly restores the synchronized state in our theory. We calculate that this “cell-body rocking” provides the dominant contribution to synchronization in swimming cells, whereas direct hydrodynamic interactions between the flagella contribute negligibly. We experimentally confirmed the two-way coupling between flagellar beating and cell-body rocking predicted by our theory. PMID:24145440

  18. Quasistatic Shock Waves: A Mechanism for Nonuniform Compaction in Porous Rock

    SciTech Connect

    OLSSON,WILLIAM A.

    2000-09-08

    Recent studies have observed compaction zones pass through porous rock under axisymmetric compression. An initially thin, compacted layer appears at the yield point of the stress-strain curve and then grows by thickening in the direction of maximum compression at constant stress. Strain localization theory has been applied to compaction to explain the formation of these features. This paper describes the growth of the compaction zones, that is, the propagation of their boundaries, in terms of shock wave analysis. The ratio of the applied shortening rate to the velocity of the boundary is related to the porosity change across the boundary. Certain features of the stress-strain curve are explained by the model.

  19. Feldspathic rocks on Mars: Compositional constraints from infrared spectroscopy and possible formation mechanisms

    NASA Astrophysics Data System (ADS)

    Rogers, A. Deanne; Nekvasil, Hanna

    2015-04-01

    Rare feldspar-dominated surfaces on Mars were previously reported based on near-infrared (NIR) spectral data and were interpreted to consist of anorthosite or felsic rocks. Using thermal infrared (TIR) data over the feldspar detections with the largest areal extent in Nili Patera and Noachis Terra, we rule out felsic interpretations. Basaltic or anorthositic compositions are consistent with TIR measurements, but the geologic contexts for these regions do not support a plutonic origin. Laboratory NIR spectral measurements demonstrate that large plagioclase crystals (>~840 µm) can be detected in mixtures with as much as 50 vol % mafics, which is higher than the previously stated requirement of no more than 15% mafics. Thus, anorthositic or felsic interpretations need not be invoked for all NIR-based feldspar detections. Plagioclase-enriched basaltic eruptive products can be formed from Martian basalts through partial crystallization at the base of a thick crust, followed by low-pressure crystallization of the residual liquids.

  20. Laboratory determination of mechanical properties of rocks from the Parcperdue geopressured/geothermal site

    SciTech Connect

    Sinha, K.P.; Borschel, T.F.; Holland, M.T.; Schatz, J.F.; Bebout, D.G.; Bachman, A.L.

    1981-01-01

    The deformational behavior and fluid flow characteristics of rock samples obtained from DOW/DOE L.R. Sweezy No. 1 Test Well at the Parcperdue Geopressured/Geothermal Site have been investigated in the laboratory. Elastic moduli, compressibility, uniaxial compaction coefficient, strength, creep parameters, permeability, acoustic velocites (all at reservoir conditions) and changes in these quantities induced by simulated reservoir production have been obtained from tests on several sandstone and shale samples from different depths. Tests consisting of several hydrostatic and triaxial loading phases and pore pressure reduction were designed to provide measurements to be used for calculating several of the above mentioned parameters in a single test. Pore volume changes were measured during some phases of the tests.

  1. Comets: mechanisms of x-ray activity

    NASA Astrophysics Data System (ADS)

    Ibadov, Subhon

    2016-07-01

    Basic mechanisms of X-ray activity of comets are considered, including D-D mechanism corresponding to generation of X-rays due to production of hot short-living plasma clumps at high-velocity collisions between cometary and interplanetary dust particles as well as M-M one corresponding to production of X-rays due to recombination of multicharge ions of solar wind plasma via charge exchange process at their collisions with molecules/atoms of the cometary atmospheres. Peculiarities of the variation of the comet X-ray spectrum and X-ray luminosity with variation of its heliocentric distance are revealed.

  2. Caspase-3 dependent nitrergic neuronal apoptosis following cavernous nerve injury is mediated via RhoA and ROCK activation in major pelvic ganglion

    PubMed Central

    Hannan, Johanna L.; Matsui, Hotaka; Sopko, Nikolai A.; Liu, Xiaopu; Weyne, Emmanuel; Albersen, Maarten; Watson, Joseph W.; Hoke, Ahmet; Burnett, Arthur L.; Bivalacqua, Trinity J.

    2016-01-01

    Axonal injury due to prostatectomy leads to Wallerian degeneration of the cavernous nerve (CN) and erectile dysfunction (ED). Return of potency is dependent on axonal regeneration and reinnervation of the penis. Following CN injury (CNI), RhoA and Rho-associated protein kinase (ROCK) increase in penile endothelial and smooth muscle cells. Previous studies indicate that nerve regeneration is hampered by activation of RhoA/ROCK pathway. We evaluated the role of RhoA/ROCK pathway in CN regulation following CNI using a validated rat model. CNI upregulated gene and protein expression of RhoA/ROCK and caspase-3 mediated apoptosis in the major pelvic ganglion (MPG). ROCK inhibitor (ROCK-I) prevented upregulation of RhoA/ROCK pathway as well as activation of caspase-3 in the MPG. Following CNI, there was decrease in the dimer to monomer ratio of neuronal nitric oxide synthase (nNOS) protein and lowered NOS activity in the MPG, which were prevented by ROCK-I. CNI lowered intracavernous pressure and impaired non-adrenergic non-cholinergic-mediated relaxation in the penis, consistent with ED. ROCK-I maintained the intracavernous pressure and non-adrenergic non-cholinergic-mediated relaxation in the penis following CNI. These results suggest that activation of RhoA/ROCK pathway mediates caspase-3 dependent apoptosis of nitrergic neurons in the MPG following CNI and that ROCK-I can prevent post-prostatectomy ED. PMID:27388816

  3. Caspase-3 dependent nitrergic neuronal apoptosis following cavernous nerve injury is mediated via RhoA and ROCK activation in major pelvic ganglion.

    PubMed

    Hannan, Johanna L; Matsui, Hotaka; Sopko, Nikolai A; Liu, Xiaopu; Weyne, Emmanuel; Albersen, Maarten; Watson, Joseph W; Hoke, Ahmet; Burnett, Arthur L; Bivalacqua, Trinity J

    2016-01-01

    Axonal injury due to prostatectomy leads to Wallerian degeneration of the cavernous nerve (CN) and erectile dysfunction (ED). Return of potency is dependent on axonal regeneration and reinnervation of the penis. Following CN injury (CNI), RhoA and Rho-associated protein kinase (ROCK) increase in penile endothelial and smooth muscle cells. Previous studies indicate that nerve regeneration is hampered by activation of RhoA/ROCK pathway. We evaluated the role of RhoA/ROCK pathway in CN regulation following CNI using a validated rat model. CNI upregulated gene and protein expression of RhoA/ROCK and caspase-3 mediated apoptosis in the major pelvic ganglion (MPG). ROCK inhibitor (ROCK-I) prevented upregulation of RhoA/ROCK pathway as well as activation of caspase-3 in the MPG. Following CNI, there was decrease in the dimer to monomer ratio of neuronal nitric oxide synthase (nNOS) protein and lowered NOS activity in the MPG, which were prevented by ROCK-I. CNI lowered intracavernous pressure and impaired non-adrenergic non-cholinergic-mediated relaxation in the penis, consistent with ED. ROCK-I maintained the intracavernous pressure and non-adrenergic non-cholinergic-mediated relaxation in the penis following CNI. These results suggest that activation of RhoA/ROCK pathway mediates caspase-3 dependent apoptosis of nitrergic neurons in the MPG following CNI and that ROCK-I can prevent post-prostatectomy ED.

  4. Sinomenine alleviates high glucose-induced renal glomerular endothelial hyperpermeability by inhibiting the activation of RhoA/ROCK signaling pathway.

    PubMed

    Yin, Qingqiao; Xia, Yuanyu; Wang, Guan

    2016-09-01

    As an early sign of diabetic cardiovascular disease, endothelial dysfunction may contribute to progressive diabetic nephropathy (DN). Endothelial hyperpermeability induced by hyperglycemia (HG) is a central pathogenesis for DN. Sinomenine (SIN) has strong anti-inflammatory and renal protective effects, following an unknown protective mechanism against HG-induced hyperpermeability. We herein explored the role of SIN in vitro in an HG-induced barrier dysfunction model in human renal glomerular endothelial cells (HRGECs). The cells were exposed to SIN and/or HG for 24 h, the permeability of which was significantly increased by HG. Moreover, junction protein occludin in the cell-cell junction area and its total expression in HRGECs were significantly decreased by HG. However, the dysfunction of tight junction and hyperpermeability of HRGECs were significantly reversed by SIN. Furthermore, SIN prevented HG-increased reactive oxygen species (ROS) by activating nuclear factor-E2-related factor 2 (Nrf2). Interestingly, activation of RhoA/ROCK induced by HG was reversed by SIN or ROCK inhibitor. HG-induced hyperpermeability was prevented by SIN. High ROS level, tight junction dysfunction and RhoA/ROCK activation were significantly attenuated with knockdown of Nrf2. Mediated by activation of Nrf2, SIN managed to significantly prevent HG-disrupted renal endothelial barrier function by suppressing the RhoA/ROCK signaling pathway through reducing ROS. We successfully identified a novel pathway via which SIN exerted antioxidative and renal protective functions, and provided a molecular basis for potential SIN applications in treating DN vascular disorders.

  5. Sinomenine alleviates high glucose-induced renal glomerular endothelial hyperpermeability by inhibiting the activation of RhoA/ROCK signaling pathway.

    PubMed

    Yin, Qingqiao; Xia, Yuanyu; Wang, Guan

    2016-09-01

    As an early sign of diabetic cardiovascular disease, endothelial dysfunction may contribute to progressive diabetic nephropathy (DN). Endothelial hyperpermeability induced by hyperglycemia (HG) is a central pathogenesis for DN. Sinomenine (SIN) has strong anti-inflammatory and renal protective effects, following an unknown protective mechanism against HG-induced hyperpermeability. We herein explored the role of SIN in vitro in an HG-induced barrier dysfunction model in human renal glomerular endothelial cells (HRGECs). The cells were exposed to SIN and/or HG for 24 h, the permeability of which was significantly increased by HG. Moreover, junction protein occludin in the cell-cell junction area and its total expression in HRGECs were significantly decreased by HG. However, the dysfunction of tight junction and hyperpermeability of HRGECs were significantly reversed by SIN. Furthermore, SIN prevented HG-increased reactive oxygen species (ROS) by activating nuclear factor-E2-related factor 2 (Nrf2). Interestingly, activation of RhoA/ROCK induced by HG was reversed by SIN or ROCK inhibitor. HG-induced hyperpermeability was prevented by SIN. High ROS level, tight junction dysfunction and RhoA/ROCK activation were significantly attenuated with knockdown of Nrf2. Mediated by activation of Nrf2, SIN managed to significantly prevent HG-disrupted renal endothelial barrier function by suppressing the RhoA/ROCK signaling pathway through reducing ROS. We successfully identified a novel pathway via which SIN exerted antioxidative and renal protective functions, and provided a molecular basis for potential SIN applications in treating DN vascular disorders. PMID:27378427

  6. Effects of fluid-rock interactions on faulting within active fault zones - evidence from fault rock samples retrieved from international drilling projects

    NASA Astrophysics Data System (ADS)

    Janssen, C.; Wirth, R.; Kienast, M.; Yabe, Y.; Sulem, J.; Dresen, G. H.

    2015-12-01

    Chemical and mechanical effects of fluids influence the fault mechanical behavior. We analyzed fresh fault rocks from several scientific drilling projects to study the effects of fluids on fault strength. For example, in drill core samples on a rupture plane of an Mw 2.2 earthquake in a deep gold mine in South Africa the main shock occurred on a preexisting plane of weakness that was formed by fluid-rock interaction (magnesiohornblende was intensively altered to chlinochlore). The plane acted as conduit for hydrothermal fluids at some time in the past. The chemical influence of fluids on mineralogical alteration and geomechanical processes in fault core samples from SAFOD (San Andreas Fault Observatory at Depth) is visible in pronounced dissolution-precipitation processes (stylolites, solution seams) as well as in the formation of new phases. Detrital quartz and feldspar grains are partially dissolved and replaced by authigenic illite-smectite (I-S) mixed-layer clay minerals. Transmission Electron Microscopy (TEM) imaging of these grains reveals that the alteration processes and healing were initiated within pores and small intra-grain fissures. Newly formed phyllosilicates growing into open pore spaces likely reduced the fluid permeability. The mechanical influence of fluids is indicated by TEM observations, which document open pores that formed in-situ in the gouge material during or after deformation. Pores were possibly filled with formation water and/or hydrothermal fluids suggesting elevated fluid pressure preventing pore collapse. Fluid-driven healing of fractures in samples from SAFOD and the DGLab Gulf of Corinth project is visible in cementation. Cathodoluminescence microscopy (CL) reveals different generations of calcite veins. Differences in CL-colors suggest repeated infiltration of fluids with different chemical composition from varying sources (formation and meteoric water).

  7. Exhumation of UHP/LT rocks due to the local reduction of the interplate pressure: Thermo-mechanical physical modelling

    NASA Astrophysics Data System (ADS)

    Boutelier, David A.; Chemenda, Alexander I.

    2008-07-01

    Spatial distribution of UHP/LT terrains suggests that their exhumation is essentially a three-dimensional process that occurs only locally in specific sites along mountain belts. On the other hand, the continental subduction resulting in the formation of UHP/LT rocks takes place along the whole belt. The previously performed by the authors 2-D thermo-mechanical laboratory modelling of continental subduction has shown that exhumation of deeply subducted continental crust is possible only when the effective interplate pressure pn is lower than the lithostatic pressure (low compression subduction regime). At the same time, this modelling showed that for the deeply subducted continental crust to be preserved at low temperature at great depth, the continental subduction should be accompanied by the subduction of the fore-arc block or the arc plate. The latter process occurs only when pn is high (high compression regime). To reconcile both processes we suggest that within the background of a generally high compression regime the interplate pressure can be locally reduced in some specific situations which would then allow the local exhumation of UHP/LT material. Using physical modelling technique we investigate one of such situations that occurs when the frontal part of the overriding plate undergoes (subduction induced or not) extension parallel to the plate boundary with activation or formation of a strike-slip transform fault oblique to the plate boundary (to the interplate zone). The displacement along this fault results in a local reduction of the interplate pressure at the intersection of the fault with the interplate zone. This pressure reduction permits the rise of the deeply subducted low-density continental crust and sediments submitted to UHP/LT conditions under buoyancy force. A 10 km-thick slice of crust detaches at ~ 150 km-depth and moves up along the interplate zone with a starting rate of ca. 3 cm/yr. The ascent rate reduces when the unit reaches crustal

  8. Design, Synthesis, and Structure-Activity Relationships of Pyridine-Based Rho Kinase (ROCK) Inhibitors.

    PubMed

    Green, Jeremy; Cao, Jingrong; Bandarage, Upul K; Gao, Huai; Court, John; Marhefka, Craig; Jacobs, Marc; Taslimi, Paul; Newsome, David; Nakayama, Tomoko; Shah, Sundeep; Rodems, Steve

    2015-06-25

    The Rho kinases (ROCK1 and ROCK2) are highly homologous serine/threonine kinases that act on substrates associated with cellular motility, morphology, and contraction and are of therapeutic interest in diseases associated with cellular migration and contraction, such as hypertension, glaucoma, and erectile dysfunction. Beginning with compound 4, an inhibitor of ROCK1 identified through high-throughput screening, systematic exploration of SAR, and application of structure-based design, led to potent and selective ROCK inhibitors. Compound 37 represents significant improvements in inhibition potency, kinase selectivity, and CYP inhibition and possesses pharmacokinetics suitable for in vivo experimentation. PMID:26039570

  9. A coupled thermo-poro-mechanical finite element analysis of fractured porous rocks using a cohesive interface element

    NASA Astrophysics Data System (ADS)

    Wang, W.; Regueiro, R. A.

    2014-12-01

    The coupling between multiphase flow, heat transfer, and poromechanics in fractured geomaterials has aroused great interest in the areas of geomechanics, geoenvironmental engineering, and petroleum engineering. Relevant applications include nuclear waste repositories, geological sequestration of CO2, geothermal systems, and exploitation of shale gas reservoirs. The paper presents a fully coupled thermo-poro-mechanical (TPM) cohesive interface element (CIE) model, which can represent fluid and heat flow along and across the fracture, and shear/normal deformation of the fracture surfaces. The proposed model is then applied to analyze two popular geological engineering problems using the finite element method (FEM) with a small strain formulation. The first application is the fracturing process in organic-rich shale due to heating. In the finite element analysis, multiple horizontal microcracks parallel to the bedding plane are assumed to preexist in the porous source rock, and are represented by coupled TPM cohesive interface elements. The porous bulk rock is assumed to be homogeneous, isotropic (for the time being, with transverse isotropy a natural extension), and linearly elastic. The excess pore fluid pressure, which mainly causes the development of the fractures, is actually induced by the rapid decomposition of organic matter during heating according to the literature. However, the involved complex chemical reaction process is beyond the scope of the paper, and is therefore substituted by a fluid injection process within the cracks under room temperature (25C) and high temperature (400C) in the paper. We investigate the fracture propagation due to pore fluid pressure increase and the development of fracture-induced permeability. The second application is a nuclear waste repository in a partially saturated fractured rock. Multiphase transport of moisture and heat, thermally-induced stress, as well as the change of fracture apertures are investigated due to short

  10. Phosphatase Activities of Endolithic Communities in Rocks of the Antarctic Dry Valleys.

    PubMed

    Banerjee; Whitton; Wynn-Williams

    2000-01-01

    Phosphorus is scarce in Beacon Sandstone of the McMurdo Dry Valleys, Antarctica, and any input from precipitation is minimal. In endolithic microbial communities recycling of P by the action of phosphatases may therefore be important. The phosphatase activities of three different types of endolithic communities in the McMurdo Dry Valley, Antarctica, were studied in the laboratory. The dominant phototrophs were Chroococcidiopsis, mixed Gloeocapsa and Trebouxia, and Trebouxia. Bacteria were also visually conspicuous in the latter two communities, and the Trebouxia in both cases formed a lichenized association with fungal hyphae. In each case marked phosphomonoesterase (PMEase) activity was found in assays with 4-methylumbelliferyl phosphate (MUP) or p-nitrophenyl phosphate as substrate, and phosphodiesterase activity with bis-p-nitrophenyl phosphate as substrate. The pH optimum of PMEase (assayed at 0.5 pH intervals) of the Chroococcidiopsis, Gloeocapsa-Trebouxia, and Trebouxia communities was 9.5, 5.5, and 8.0, respectively. These values are similar for aqueous extracts of the respective rocks (pH 9.2, 6.2, 7.5). All three communities showed significantly higher PMEase activity at 5 degrees than 1 degrees C, and the first two also showed much higher activity at 5 degrees than 10 degrees C. All three communities also showed slightly lower activity in the light (7 µmol photon m(-2) s(-1)) than the dark; this was found with all substrates and substrate concentrations. Prior exposure of a moistened sample to light for 2 h led to a reduction in activity even when the subsequent assay was done in the dark. The rate of PMEase activity (using 100 µM MUP) in the Gloeocapsa-Trebouxia and Trebouxia communities was approximately linear with time up to 24 h, whereas the Chroococcidiopsis community showed a marked decrease after 6 h. At least part of this was due to retention of the 4-methylumbelliferone (MU) hydrolysis product. In spite of the assays being conducted on a whole

  11. Phosphatase Activities of Endolithic Communities in Rocks of the Antarctic Dry Valleys.

    PubMed

    Banerjee; Whitton; Wynn-Williams

    2000-01-01

    Phosphorus is scarce in Beacon Sandstone of the McMurdo Dry Valleys, Antarctica, and any input from precipitation is minimal. In endolithic microbial communities recycling of P by the action of phosphatases may therefore be important. The phosphatase activities of three different types of endolithic communities in the McMurdo Dry Valley, Antarctica, were studied in the laboratory. The dominant phototrophs were Chroococcidiopsis, mixed Gloeocapsa and Trebouxia, and Trebouxia. Bacteria were also visually conspicuous in the latter two communities, and the Trebouxia in both cases formed a lichenized association with fungal hyphae. In each case marked phosphomonoesterase (PMEase) activity was found in assays with 4-methylumbelliferyl phosphate (MUP) or p-nitrophenyl phosphate as substrate, and phosphodiesterase activity with bis-p-nitrophenyl phosphate as substrate. The pH optimum of PMEase (assayed at 0.5 pH intervals) of the Chroococcidiopsis, Gloeocapsa-Trebouxia, and Trebouxia communities was 9.5, 5.5, and 8.0, respectively. These values are similar for aqueous extracts of the respective rocks (pH 9.2, 6.2, 7.5). All three communities showed significantly higher PMEase activity at 5 degrees than 1 degrees C, and the first two also showed much higher activity at 5 degrees than 10 degrees C. All three communities also showed slightly lower activity in the light (7 µmol photon m(-2) s(-1)) than the dark; this was found with all substrates and substrate concentrations. Prior exposure of a moistened sample to light for 2 h led to a reduction in activity even when the subsequent assay was done in the dark. The rate of PMEase activity (using 100 µM MUP) in the Gloeocapsa-Trebouxia and Trebouxia communities was approximately linear with time up to 24 h, whereas the Chroococcidiopsis community showed a marked decrease after 6 h. At least part of this was due to retention of the 4-methylumbelliferone (MU) hydrolysis product. In spite of the assays being conducted on a whole

  12. Rocks in Our Pockets

    ERIC Educational Resources Information Center

    Plummer, Donna; Kuhlman, Wilma

    2005-01-01

    To introduce students to rocks and their characteristics, teacher can begin rock units with the activities described in this article. Students need the ability to make simple observations using their senses and simple tools.

  13. Reaction zone between pre-UHP titanite and host rock: insights into fluid-rock interaction and deformation mechanisms during exhumation of deeply subducted continental crust (Dabie Shan UHP unit, China)

    NASA Astrophysics Data System (ADS)

    Wawrzenitz, N.; Romer, R. L.; Grasemann, B.; Morales, L. F. G.

    2012-04-01

    Exhumed crustal UHP rocks may occur as relict blocks in strongly metasomatized matrix rocks. Due to variations in competence between the mm to km sized blocks and their ductile matrix, the largely undeformed blocks may preserve the pre-subduction and the prograde history, whereas the matrix rocks have been ductilely deformed to high magnitudes and record successive stages of deformation. The reaction zones between blocks and matrix, however, provide insights into the fluid-rock interaction, deformation and the deformation mechanisms active during the exhumation of deeply subducted continental crust in the subduction channel. We investigate a titanite megacryst (3 cm in diameter) in a calc-silicate marble from the UHP unit in the Dabie Shan, China. The core of the titanite megacryst grew prograde during subduction. Its U-Pb system remained closed and yields a maximum age for UHP metamorphism. Sr and Nd isotope compositions in the core demonstrate that the titanite megacryst precipitated from a homogeneous fluid source. During metamorphism in the subduction zone, infiltration of external fluids resulted first in Sr-loss from the marbles and then introduction of Sr with unusually low 87Sr/86S values (Romer et al., 2003), leading to the contrasting 87Sr/86Sr values in the titanite megacryst and the hosting UHP marbles (Wawrzenitz et al., 2006). Related to deformation in the calc-silicate marble matrix, the rim of the titanite megacryst has been replaced during the following dissolution-precipitation reactions: (i) Pseudomorphic replacement of the old titanite megacryst by coupled dissolution-reprecipitation. Fluid migrated into the old grain producing a sharp boundary of the replacement front. (ii) New small titanite grains grew with their long axes parallel to the foliation of the marble matrix, reflecting the activation of dissolution precipitation creep. In the matrix, the foliation is defined by the orientation of the basal planes of phengitic white mica. The new

  14. Topological mechanics: from metamaterials to active matter

    NASA Astrophysics Data System (ADS)

    Vitelli, Vincenzo

    2015-03-01

    Mechanical metamaterials are artificial structures with unusual properties, such as negative Poisson ratio, bistability or tunable acoustic response, which originate in the geometry of their unit cell. At the heart of such unusual behavior is often a mechanism: a motion that does not significantly stretch or compress the links between constituent elements. When activated by motors or external fields, these soft motions become the building blocks of robots and smart materials. In this talk, we discuss topological mechanisms that possess two key properties: (i) their existence cannot be traced to a local imbalance between degrees of freedom and constraints (ii) they are robust against a wide range of structural deformations or changes in material parameters. The continuum elasticity of these mechanical structures is captured by non-linear field theories with a topological boundary term similar to topological insulators and quantum Hall systems. We present several applications of these concepts to the design and experimental realization of 2D and 3D topological structures based on linkages, origami, buckling meta-materials and lastly active media that break time-reversal symmetry.

  15. Mechanically activated artificial cell by using microfluidics

    PubMed Central

    Ho, Kenneth K. Y.; Lee, Lap Man; Liu, Allen P.

    2016-01-01

    All living organisms sense mechanical forces. Engineering mechanosensitive artificial cell through bottom-up in vitro reconstitution offers a way to understand how mixtures of macromolecules assemble and organize into a complex system that responds to forces. We use stable double emulsion droplets (aqueous/oil/aqueous) to prototype mechanosensitive artificial cells. In order to demonstrate mechanosensation in artificial cells, we develop a novel microfluidic device that is capable of trapping double emulsions into designated chambers, followed by compression and aspiration in a parallel manner. The microfluidic device is fabricated using multilayer soft lithography technology, and consists of a control layer and a deformable flow channel. Deflections of the PDMS membrane above the main microfluidic flow channels and trapping chamber array are independently regulated pneumatically by two sets of integrated microfluidic valves. We successfully compress and aspirate the double emulsions, which result in transient increase and permanent decrease in oil thickness, respectively. Finally, we demonstrate the influx of calcium ions as a response of our mechanically activated artificial cell through thinning of oil. The development of a microfluidic device to mechanically activate artificial cells creates new opportunities in force-activated synthetic biology. PMID:27610921

  16. Mechanically activated artificial cell by using microfluidics.

    PubMed

    Ho, Kenneth K Y; Lee, Lap Man; Liu, Allen P

    2016-01-01

    All living organisms sense mechanical forces. Engineering mechanosensitive artificial cell through bottom-up in vitro reconstitution offers a way to understand how mixtures of macromolecules assemble and organize into a complex system that responds to forces. We use stable double emulsion droplets (aqueous/oil/aqueous) to prototype mechanosensitive artificial cells. In order to demonstrate mechanosensation in artificial cells, we develop a novel microfluidic device that is capable of trapping double emulsions into designated chambers, followed by compression and aspiration in a parallel manner. The microfluidic device is fabricated using multilayer soft lithography technology, and consists of a control layer and a deformable flow channel. Deflections of the PDMS membrane above the main microfluidic flow channels and trapping chamber array are independently regulated pneumatically by two sets of integrated microfluidic valves. We successfully compress and aspirate the double emulsions, which result in transient increase and permanent decrease in oil thickness, respectively. Finally, we demonstrate the influx of calcium ions as a response of our mechanically activated artificial cell through thinning of oil. The development of a microfluidic device to mechanically activate artificial cells creates new opportunities in force-activated synthetic biology. PMID:27610921

  17. Mechanically activated artificial cell by using microfluidics.

    PubMed

    Ho, Kenneth K Y; Lee, Lap Man; Liu, Allen P

    2016-01-01

    All living organisms sense mechanical forces. Engineering mechanosensitive artificial cell through bottom-up in vitro reconstitution offers a way to understand how mixtures of macromolecules assemble and organize into a complex system that responds to forces. We use stable double emulsion droplets (aqueous/oil/aqueous) to prototype mechanosensitive artificial cells. In order to demonstrate mechanosensation in artificial cells, we develop a novel microfluidic device that is capable of trapping double emulsions into designated chambers, followed by compression and aspiration in a parallel manner. The microfluidic device is fabricated using multilayer soft lithography technology, and consists of a control layer and a deformable flow channel. Deflections of the PDMS membrane above the main microfluidic flow channels and trapping chamber array are independently regulated pneumatically by two sets of integrated microfluidic valves. We successfully compress and aspirate the double emulsions, which result in transient increase and permanent decrease in oil thickness, respectively. Finally, we demonstrate the influx of calcium ions as a response of our mechanically activated artificial cell through thinning of oil. The development of a microfluidic device to mechanically activate artificial cells creates new opportunities in force-activated synthetic biology.

  18. Partial reactivation of a huge deep-seated ancient rock slide: recognition, formation mechanism, and stability

    NASA Astrophysics Data System (ADS)

    Tang, Minggao; Xu, Qiang; Li, Yusheng; Huang, Runqiu; Rengers, Niek; Zhu, Xing

    2016-08-01

    About 18 years ago, a large-scale discontinuous layer in properties and colour was found in the new Fengjie town at the shore of the Three Gorges Reservoir area in China. There are many resettled residents and buildings on the sloping area, the safety of which is potentially affected by this layer, so it has become the focus of attention. Before this study started there were two viewpoints regarding the origin of this layer. One was that is was from a huge ancient slide and the other was that is was from a fault graben. In order to find out how it was formed and to be able to carry out a stability analysis of the slope the authors have carried out a research program, including geological field investigations and mapping, a deep drilling hole, a geotechnical centrifuge model test, and a simulation analysis. The results of the research led to the conclusion that the layer is the sliding plane of a huge deep-seated ancient rock slide, which we called the Sanmashan landslide. An important argument for the conclusion is the recognition of a regional compressive tectonic stress field in this area, which cannot lead to the formation of a fault graben because it needs a tensional tectonic stress field. Moreover, numerous unique geological features, sliding marks, and other relics of the ancient slide have been discovered in the field. The formation process of the ancient slide could be repeated in a large geotechnical centrifuge model test. The test shows that a deformation and failure process of "creep-crack-cut" has occurred. The type of the ancient slide can be classified as a "successive rotational rock slide". Finally, the role of seepage in the stability of the Sanmashan landslide has been analysed. Our final conclusions are that, during rainfall and filling-drawdown cycles in the Three Gorges Reservoir, the Sanmashan landslide as a whole is dormant and stable and the secondary landslides in the toe area of the slope are presently stable but can be reactivated. This

  19. Flow mechanism of self-induced reversed limit-cycle wing rock for a chined forebody configuration

    NASA Astrophysics Data System (ADS)

    Shi, Wei; Deng, Xueying; Wang, Yankui; Li, Qian

    2015-11-01

    The wing rock phenomenon reduces the maneuverability and affects the flight safety of modern advanced fighters, such as the F-35, which have chined forebodies. Understanding the flow mechanism is critical to suppressing this phenomenon. In this study, experiments were conducted to reveal the motion and flow behavior over a chined forebody configuration. The tests were performed in a wind tunnel at an angle of attack of 50∘ with a Reynolds number of 1.87 × 105. Reversed limit-cycle oscillation was discovered in the free-to-roll tests. The unstable rolling moment around zero roll angle in the static case suggests that the model tends to be driven away from zero roll angle. Thus, the model cannot maintain its equilibrium at zero roll angle during free-to-roll motion. The unstable rolling moment is generated by the wing vortex structure above the upward wing, which is induced by the forebody asymmetric vortices. During wing rock, the wing vortex structure appears above the upward wing at a large roll angle after crossing zero roll angle owing to a time lag in the forebody vortex position, which is conducive to the motion. The forebody asymmetric vortices are thus the key to induce and maintain the motion.

  20. Between a rock and a soft place: recent progress in understanding matrix mechanics.

    PubMed

    Moraes, Christopher

    2015-07-01

    The mechanical properties of a cell's surrounding environment play a critical role in modulating cell function, particularly in disease progression. Although simple model systems to study these phenomena have been developed over the last 15 years, several studies indicate that simple models may not adequately capture the complexity surrounding the interplay between matrix mechanics and stiffness. Here, we highlight recent advances in novel technologies, material design strategies, and bioanalytical approaches that have shed new light on the complex interplay between materials, mechanics and biological function.

  1. Warm Eocene climate enhanced petroleum generation from Cretaceous source rocks: A potential climate feedback mechanism?

    NASA Astrophysics Data System (ADS)

    Kroeger, K. F.; Funnell, R. H.

    2012-02-01

    Earth surface temperatures, including in the deep sea increased by 5-10°C from the late Paleocene ca. 58 Myr ago to the Early Eocene Climatic Optimum (EECO) centered at about 51 Myr ago. A large (˜2.5‰) drop in δ13C of carbonate spans much of this interval. This suggests a long-term increase in the net flux of 13C-depleted carbon to the ocean and atmosphere that is difficult to explain by changes in surficial carbon cycling alone. We reveal a relationship between surface temperature increase and increased petroleum generation in sedimentary basins operating on 100 kyr to Myr time scales. We propose that early Eocene warming has led to a synchronization of periods of maximum petroleum generation and enhanced generation in otherwise unproductive basins through extension of the volume of source rock within the oil and gas window across hundreds of sedimentary basins globally. Modelling the thermal evolution of four sedimentary basins in the southwest Pacific predicted an up to 50% increase in petroleum generation that would have significantly increased leakage of light hydrocarbons and oil degeneration products into the atmosphere. Extrapolating our modelling results to hundreds of sedimentary basins worldwide suggests that globally increased leakage could have caused a climate feedback effect, driving or enhancing early Eocene climate warming.

  2. Real-time Observations of Rock Cracking and Weather Provide Insights into Thermal Stress-Related Processes of Mechanical Weathering.

    NASA Astrophysics Data System (ADS)

    Eppes, M. C.; Magi, B. I.; Keanini, R.

    2015-12-01

    The environmental conditions (weather and/or climate) that limit or drive mechanical weathering via thermal stress are poorly understood. Here we examine acoustic emission (AE) records of rock cracking in boulders sitting on the ground in humid-temperate (~1 year of data) and semi-arid (~3 years) locations. We compare on-site average ambient daily temperature for days in which cracking occurs to the average temperatures for those dates derived from local climate records. The temperatures characterizing days on which cracking occurs is similar for both stations (range = -10 C to +30 C); where 21% and 73% of cracking occurs on hot days (> 20C) in the humid and semi-arid climates respectively while 17% and 0.1% occurs on very cold days (-8C to -3C). When days during which cracking occurs are compared to climate averages, 81% (NC) and 51% (NM) of all cracking occurs on days with absolute temperature anomalies >1, regardless of the temperature. The proportion of cracking that occurs on anomalously hot or cold days rises to 92% and 77% when the data is normalized to account for uneven sampling of the days with extreme temperatures. In order to determine to what extent this trend holds true in a more complex setting, we examined an existing 100+ year record of rock falls from Yosemite Valley. Preliminary results, although more equivocal, are consistent with the boulder cracking AE data. We examine the AE datasets in the context of our previous numerical modeling of insolation-driven thermal stress in rock and hypothesize that there is an increased potential for fracture on days with extreme temperatures because 1) thermal-stress is dependent on temperature variance from far-field and/or average rock temperatures and 2) that days with climatologically extreme air temperatures result in maximums in such variance. An implication of our results is that environments with extreme weather variability may have higher thermal breakdown rates, including certain locations today and

  3. Semi-active control of the rocking motion of monolithic art objects

    NASA Astrophysics Data System (ADS)

    Ceravolo, Rosario; Pecorelli, Marica Leonarda; Zanotti Fragonara, Luca

    2016-07-01

    The seismic behaviour of many art objects and obelisks can be analysed in the context of the seismic response of rigid blocks. Starting from the pioneering works by Housner, a large number of analytical studies of the rigid block dynamics were proposed. In fact, despite its apparent simplicity, the motion of a rigid block involves a number of complex dynamic phenomena such as impacts, sliding, uplift and geometric nonlinearities. While most of the current strategies to avoid toppling consist in preventing rocking motion, in this paper a novel semi-active on-off control strategy for protecting monolithic art objects was investigated. The control procedure under study follows a feedback-feedforward scheme that is realised by switching the stiffness of the anchorages located at the two lower corner of the block between two values. Overturning spectra have been calculated in order to clarify the benefits of applying a semi-active control instead of a passive control strategy. In accordance with similar studies, the numerical investigation took into account the dynamic response of blocks with different slenderness and size subject to one-sine pulse excitation.

  4. Streaming potential modeling in fractured rock: Insights into the identification of hydraulically active fractures

    NASA Astrophysics Data System (ADS)

    Roubinet, D.; Linde, N.; Jougnot, D.; Irving, J.

    2016-05-01

    Numerous field experiments suggest that the self-potential (SP) geophysical method may allow for the detection of hydraulically active fractures and provide information about fracture properties. However, a lack of suitable numerical tools for modeling streaming potentials in fractured media prevents quantitative interpretation and limits our understanding of how the SP method can be used in this regard. To address this issue, we present a highly efficient two-dimensional discrete-dual-porosity approach for solving the fluid flow and associated self-potential problems in fractured rock. Our approach is specifically designed for complex fracture networks that cannot be investigated using standard numerical methods. We then simulate SP signals associated with pumping conditions for a number of examples to show that (i) accounting for matrix fluid flow is essential for accurate SP modeling and (ii) the sensitivity of SP to hydraulically active fractures is intimately linked with fracture-matrix fluid interactions. This implies that fractures associated with strong SP amplitudes are likely to be hydraulically conductive, attracting fluid flow from the surrounding matrix.

  5. Dormancy activation mechanism of tracheal stem cells

    PubMed Central

    Li, Xin; Xu, Jing-xian; Jia, Xin-Shan; Li, Wen-ya; Han, Yi-chen; Wang, En-hua; Li, Fang

    2016-01-01

    Accurate markers and molecular mechanisms of stem cell dormancy and activation are poorly understood. In this study, the anti-cancer drug, 5-fluorouracil, was used to selectively kill proliferating cells of human bronchial epithelial (HBE) cell line. This method can enrich and purify stem cell population. The dormant versus active status of stem cells was determined by phosphorylation of RNAp II Ser2. The surviving stem cells were cultured to form stem cell spheres expressing stem cell markers and transplanted into nude mice to form a teratoma. The results demonstrated the properties of stem cells and potential for multi-directional differentiation. Bisulfite sequencing polymerase chain reaction showed that demethylation of the Sox2 promoter by 5-FU resulted in Sox2 expression in the dormant stem cells. This study shows that the dormancy and activation of HBE stem cells is closely related to epigenetic modification. PMID:27009861

  6. Mechanism of FGF receptor dimerization and activation

    PubMed Central

    Sarabipour, Sarvenaz; Hristova, Kalina

    2016-01-01

    Fibroblast growth factors (fgfs) are widely believed to activate their receptors by mediating receptor dimerization. Here we show, however, that the FGF receptors form dimers in the absence of ligand, and that these unliganded dimers are phosphorylated. We further show that ligand binding triggers structural changes in the FGFR dimers, which increase FGFR phosphorylation. The observed effects due to the ligands fgf1 and fgf2 are very different. The fgf2-bound dimer structure ensures the smallest separation between the transmembrane (TM) domains and the highest possible phosphorylation, a conclusion that is supported by a strong correlation between TM helix separation in the dimer and kinase phosphorylation. The pathogenic A391E mutation in FGFR3 TM domain emulates the action of fgf2, trapping the FGFR3 dimer in its most active state. This study establishes the existence of multiple active ligand-bound states, and uncovers a novel molecular mechanism through which FGFR-linked pathologies can arise. PMID:26725515

  7. Seismic response of rock joints and jointed rock mass

    SciTech Connect

    Ghosh, A.; Hsiung, S.M.; Chowdhury, A.H.

    1996-06-01

    Long-term stability of emplacement drifts and potential near-field fluid flow resulting from coupled effects are among the concerns for safe disposal of high-level nuclear waste (HLW). A number of factors can induce drift instability or change the near-field flow patterns. Repetitive seismic loads from earthquakes and thermal loads generated by the decay of emplaced waste are two significant factors. One of two key technical uncertainties (KTU) that can potentially pose a high risk of noncompliance with the performance objectives of 10 CFR Part 60 is the prediction of thermal-mechanical (including repetitive seismic load) effects on stability of emplacement drifts and the engineered barrier system. The second KTU of concern is the prediction of thermal-mechanical-hydrological (including repetitive seismic load) effects on the host rock surrounding the engineered barrier system. The Rock Mechanics research project being conducted at the Center for Nuclear Waste Regulatory Analyses (CNWRA) is intended to address certain specific technical issues associated with these two KTUs. This research project has two major components: (i) seismic response of rock joints and a jointed rock mass and (ii) coupled thermal-mechanical-hydrological (TMH) response of a jointed rock mass surrounding the engineered barrier system (EBS). This final report summarizes the research activities concerned with the repetitive seismic load aspect of both these KTUs.

  8. A Novel Transcription Mechanism Activated by Ethanol

    PubMed Central

    Lin, Xinghua; Yang, Hong; Zhang, Hongfeng; Zhou, LiChun; Guo, ZhongMao

    2013-01-01

    Solute carrier family 7, member 11 (Slc7a11) is a plasma membrane cystine/glutamate exchanger that provides intracellular cystine to produce glutathione, a major cellular antioxidant. Oxidative and endoplasmic reticulum stresses up-regulate Slc7a11 expression by activation of nuclear factor erythroid 2-related factor 2 and transcription factor 4. This study examined the effect of ethanol on Slc7a11 expression and the underlying mechanism involved. Treatment of mouse hepatic stellate cells with ethanol significantly increased Slc7a11 mRNA and protein levels. Deletion of a 20-bp DNA sequence between −2044 to −2024 upstream of the transcription start site significantly increased basal activity and completely abolished the ethanol-induced activity of the Slc7a11 promoter. This deletion did not affect Slc7a11 promoter activity induced by oxidative or endoplasmic reticulum stress. DNA sequence analysis revealed a binding motif for octamer-binding transcription factor 1 (OCT-1) in the deleted fragment. Mutation of this OCT-1 binding motif resulted in a similar effect as the deletion experiment, i.e. it increased the basal promoter activity and abolished the response to ethanol. Ethanol exposure significantly inhibited OCT-1 binding to the Slc7a11 promoter region, although it did not alter OCT-1 mRNA and protein levels. OCT-1 reportedly functions as either a transcriptional enhancer or repressor, depending on the target genes. Results from this study suggest that OCT-1 functions as a repressor on the Slc7a11 promoter and that ethanol inhibits OCT-1 binding to the Slc7a11 promoter, thereby increasing Slc7a11 expression. Taken together, inhibition of the DNA binding activity of transcriptional repressor OCT-1 is a mechanism by which ethanol up-regulates Slc711 expression. PMID:23592778

  9. Preliminary assessment of active rock slope instabilities in the high Himalaya of Bhutan

    NASA Astrophysics Data System (ADS)

    Dini, Benedetta; Manconi, Andrea; Leith, Kerry; Loew, Simon

    2016-04-01

    The small kingdom of Bhutan, nested between India and Tibet (between 88° and 92° east and 26° and 28° north), is characterised by markedly different landscapes and climatic zones. V-shaped, forest-covered valleys in the south, affected by the monsoonal rains, give gradually way to steep, barren slopes of U-shaped valleys in the drier north, host of the highest peaks, a large number of glaciers and glacial lakes. A transition zone of vegetated, elevated plateaus collects the towns in which most of the population lives. Landslides in the high Himalaya of Bhutan have not been extensively studied despite the primary and secondary hazards related to them. The regulations and restrictions to travel to and within Bhutan imposed by the government, as well as the extremely rugged terrain hinder the accessibility to remote slopes and valleys, both of which have resulted in lack of data and investigations. In this work, we aim at producing an inventory of large rock slope instabilities (> 1 million m3) across the high Himalaya of Bhutan, identifying types of failure, assessing the activity and analysing the distribution of landslides in combination with predisposing and preparatory factors, such as lithology, tectonic structures, hypsometry, deglaciation, fluvial erosive power and climate. At this stage, we rely on the information retrieved through satellite remote sensing data, i.e. medium and high resolution DEMs, optical images and space borne Synthetic Aperture Radar (SAR) data. An initial inventory was compiled based on the identification of geomorphological features associated with slope instabilities using the available Google Earth images. Moreover, we assessed the SAR data coverage and the expected geometrical distortions by assuming different sensors (ERS, Envisat, and ALOS Palsar-1). As we are mainly interested in detecting the surface deformation related to large unstable slopes by applying Differential SAR, we also computed the percentage of potentially

  10. An insight to the eruption mechanism of volcanic rocks in North Tibet: Project TITAN

    NASA Astrophysics Data System (ADS)

    He, R.; Gao, R.; Li, Q.

    2013-12-01

    Since Cenozoic continent-continental collision between India and Asia [Yin and Harrison, 2000], many surface features happened within the plateau were prominent. As one of them, high-to-ultra potassic lavas of Neogene, even younger, are widely distributed in NW Tibet, as shown in Figure1. Obvious petrological and geochemical characteristics of those lavas [Turner et al., 1993; Deng et al., 1996; Hacker et al., 2000; Ding et al., 2003; Williams et al., 2004; Chung et al., 2005; Luo et al., 2006; Guo et al., 2006] were presumably associated with extensional collapse of the lithospheric mantle [Deng et al., 1998; Li et al., 2000; Ding et al., 2003; Williams et al., 2004; Chung et al., 2005; Luo et al., 2006; Guo et al., 2006], which started at about 4Ma [Yin et al., 1999] and still goes on [Lin et al., 2002]. (1) What kinetics happened within the lithosphere beneath the lava region since Late Neogene? (2) What kind of mechanism leads to the lava eruption, it is only the result of far-long effect of northward subduction of the India plate, or directly related to the south margin of the Asian lithosphere, or bilaterally affected by India and Asia plate, or other factors? (3) Why did the strong volcanic activities only wrecked the North Qiangtang terrane, Jinshajiang suture and Hoh Xil terrane, instead of going northward across Altyn Tagh Fault and East Kunlun? As is known, to reveal the scientific secret concealed in lavas within NW Tibet geophysical observations are very necessary and urgent. NW Tibet including North Qiangtang and Hoh Xil is a very tough region for human beings. The project for geophysical observatory in the region is named as TITAN. TITAN has currently made some great progress, such as 103 broadband Seismic observation stations were deployed along longitude 88.5E in recent 5 years, as shown in Figure 1. Some primary results were got, such as (1) P-velocity structure from teleseismic tomography around Shuanghu in central Qiangtang (Figure 2) showed

  11. Microfabrics and 3D grain shape of Gorleben rock salt: Constraints on deformation mechanisms and paleodifferential stress

    NASA Astrophysics Data System (ADS)

    Thiemeyer, Nicolas; Zulauf, Gernold; Mertineit, Michael; Linckens, Jolien; Pusch, Maximilian; Hammer, Jörg

    2016-04-01

    The Permian Knäuel- and Streifensalz formations (z2HS1 and z2HS2) are main constituents of the Gorleben salt dome (Northern Germany) and show different amounts and distributions of anhydrite. The reconstruction of 3D halite grain shape ellipsoids reveals small grain size (3.4 ± 0.6 mm) and heterogeneous grain shapes in both formations, the latter attributed to the polyphase deformation of the rock salt during diapirism. The halite microfabrics of both formations indicate that strain-induced grain boundary migration was active during deformation. Crystal plastic deformation of halite is further documented by lattice bending, subgrain formation and minor subgrain rotation. Evidence for pressure solution of halite has not been found, but cannot be excluded because of the small grain size, the lack of LPO and the low differential stress (1.1-1.3 MPa) as deduced from subgrain-size piezometry. Anhydrite has been deformed in the brittle-ductile regime by solution precipitation creep, minor dislocation creep and brittle boudinage. No continuous anhydrite layers are preserved, and halite has acted as a sealing matrix embedding the disrupted anhydrite fragments prohibiting any potential migration pathways for fluids. Thus, anhydrite should not have a negative effect on the barrier properties of the Gorleben rock salts investigated in this study.

  12. Activities of the Institute for Mechanical Engineering

    NASA Astrophysics Data System (ADS)

    The Institute of Mechanical Engineering (IME) is part of Canada's National Research Council. Its mission is to undertake, support, promote, and disseminate research and development in the mechanical engineering aspects of three vital sectors of the Canadian economy: transportation, resource industries, and manufacturing. The IME achieves its mission by performing research and development in its own facilities; by developing, providing, and transferring expertise and knowledge; by making its research facilities available to collaborators and clients; and by participating in international liaison and collaborative research activities. Six research programs are conducted in the IME: Advanced Manufacturing Technology; Coastal Zone Engineering; Cold Regions Engineering; Combustion and Fluids Engineering; Ground Transportation Technology; and Machinery and Engine Technology. The rationale and major research thrusts of each program are described, and specific achievements in 1991-92 are reviewed. Lists of technical reports and papers presented by IME personnel are also included.

  13. Failure of cap-rock seals as determined from mechanical stratigraphy, stress history, and tensile-failure analysis of exhumed analogs

    DOE PAGESBeta

    Petrie, E. S.; Evans, J. P.; Bauer, S. J.

    2014-11-01

    In this study, the sedimentologic and tectonic histories of clastic cap rocks and their inherent mechanical properties control the nature of permeable fractures within them. The migration of fluid through mm- to cm-scale fracture networks can result in focused fluid flow allowing hydrocarbon production from unconventional reservoirs or compromising the seal integrity of fluid traps. To understand the nature and distribution of subsurface fluid-flow pathways through fracture networks in cap-rock seals we examine four exhumed Paleozoic and Mesozoic seal analogs in Utah. We combine these outcrop analyses with subsidence analysis, paleoloading histories, and rock-strength testing data in modified Mohr–Coulomb–Griffith analysesmore » to evaluate the effects of differential stress and rock type on fracture mode.« less

  14. Failure of cap-rock seals as determined from mechanical stratigraphy, stress history, and tensile-failure analysis of exhumed analogs

    SciTech Connect

    Petrie, E. S.; Evans, J. P.; Bauer, S. J.

    2014-11-01

    In this study, the sedimentologic and tectonic histories of clastic cap rocks and their inherent mechanical properties control the nature of permeable fractures within them. The migration of fluid through mm- to cm-scale fracture networks can result in focused fluid flow allowing hydrocarbon production from unconventional reservoirs or compromising the seal integrity of fluid traps. To understand the nature and distribution of subsurface fluid-flow pathways through fracture networks in cap-rock seals we examine four exhumed Paleozoic and Mesozoic seal analogs in Utah. We combine these outcrop analyses with subsidence analysis, paleoloading histories, and rock-strength testing data in modified Mohr–Coulomb–Griffith analyses to evaluate the effects of differential stress and rock type on fracture mode.

  15. [Mechanism of the contraceptive action of the triterpene glycosides from the rock jasmine Androsace septentrionalis L].

    PubMed

    Mats, M N; Savchenko, O N

    1986-01-01

    The sum of triterpene glycosides of Androsace septentrionalis L. (a plant of genus Primula) was shown to change the concentrations of lutropin and follitropin in the hypophysis and plasma and the ratio of these hormones in the blood plasma in the active phase of the cycle. This leads to the change of the ovarian hormonal activity in the direction of predominance of the estrogenic action and an increase of the uterine contractile activity that can be of certain significance in manifestation of the contraceptive effect.

  16. Electrical Conductivity of Rocks and Dominant Charge Carriers. Part 1; Thermally Activated Positive Holes

    NASA Technical Reports Server (NTRS)

    Freund, Friedemann T.; Freund, Minoru M.

    2012-01-01

    The prevailing view in the geophysics community is that the electrical conductivity structure of the Earth's continental crust over the 5-35 km depth range can best be understood by assuming the presence of intergranular fluids and/or of intragranular carbon films. Based on single crystal studies of melt-grown MgO, magma-derived sanidine and anorthosite feldspars and upper mantle olivine, we present evidence for the presence of electronic charge carriers, which derive from peroxy defects that are introduced during cooling, under non-equilibrium conditions, through a redox conversion of pairs of solute hydroxyl arising from dissolution of H2O.The peroxy defects become thermally activated in a 2-step process, leading to the release of defect electrons in the oxygen anion sublattice. Known as positive holes and symbolized by h(dot), these electronic charge carriers are highly mobile. Chemically equivalent to O(-) in a matrix of O(2-) they are highly oxidizing. Being metastable they can exist in the matrix of minerals, which crystallized in highly reduced environments. The h(dot) are highly mobile. They appear to control the electrical conductivity of crustal rocks in much of the 5-35 km depth range.

  17. ROCK1 and ROCK2 are required for non-small cell lung cancer anchorage-independent growth and invasion.

    PubMed

    Vigil, Dominico; Kim, Tai Young; Plachco, Ana; Garton, Andrew J; Castaldo, Linda; Pachter, Jonathan A; Dong, Hanqing; Chen, Xin; Tokar, Brianna; Campbell, Sharon L; Der, Channing J

    2012-10-15

    Evidence is emerging that the closely related ROCK1 and ROCK2 serine/threonine kinases support the invasive and metastatic growth of a spectrum of human cancer types. Therefore, inhibitors of ROCK are under preclinical development. However, a key step in their development involves the identification of genetic biomarkers that will predict ROCK inhibitor antitumor activity. One identified mechanism for ROCK activation in cancer involves the loss of function of the DLC1 tumor suppressor gene, which encodes a GTPase activating protein (RhoGAP) for the RhoA and RhoC small GTPases. DLC-1 loss may lead to hyperactivation of RhoA/C and its downstream effectors, the ROCK kinases. We therefore determined whether loss of DLC-1 protein expression identifies non-small cell lung carcinoma (NSCLC) cell lines whose growth and invasion phenotypes are sensitive to ROCK inhibition. We identified and characterized a novel small molecule pharmacologic inhibitor of ROCK and additionally applied genetic approaches to impair ROCK1 and/or ROCK2 activity, and we determined that although NSCLC anchorage-dependent growth was ROCK-independent, both anchorage-independent growth and Matrigel invasion were ROCK-dependent. However, loss of DLC-1 expression did not correlate with ROCK activation or with OXA-06 sensitivity. Unexpectedly, suppression of ROCK1 or ROCK2 expression alone was sufficient to impair anchorage-independent growth, supporting their nonoverlapping roles in oncogenesis. Mechanistically, the block in anchorage-independent growth was associated with accumulation of cells in the G(0)-G(1) phase of the cell cycle, but not increased anoikis. We conclude that ROCK may be a useful therapeutic target for NSCLC.

  18. Mechanism and active variety of allelochemicals

    USGS Publications Warehouse

    Peng, S.-L.; Wen, J.; Guo, Q.-F.

    2004-01-01

    This article summarizes allelochemicals' active variety, its potential causes and function mechanisms. Allelochemicals' activity varies with temperature, photoperiod, water and soils during natural processes, with its initial concentration, compound structure and mixed degree during functional processes, with plant accessions, tissues and maturity within-species, and with research techniques and operation processes. The prospective developmental aspects of allelopathy studies in the future are discussed. Future research should focus on: (1) to identify and purify allelochemicals more effectively, especially for agriculture, (2) the functions of allelopathy at the molecular structure level, (3) using allelopathy to explain plant species interactions, (4) allelopathy as a driving force of succession, and (5) the significance of allelopathy in the evolutionary processes.

  19. Critical Chemical-Mechanical Couplings that Define Permeability Modifications in Pressure-Sensitive Rock Fractures

    SciTech Connect

    Derek Elsworth; Abraham Grader; Susan Brantley

    2007-04-25

    This work examined and quantified processes controlling changes in the transport characteristics of natural fractures, subjected to coupled thermal-mechanical-chemical (TMC) effects. Specifically, it examined the effects of mineral dissolution and precipitation mediated by mechanical effects, using laboratory through-flow experiments concurrently imaged by X-ray CT. These were conducted on natural and artificial fractures in cores using water as the permeant. Fluid and mineral mass balances are recorded and are correlated with in-sample saturation, porosity and fracture aperture maps, acquired in real-time by X-ray CT-imaging at a maximum spatial resolution of 15-50 microns per pixel. Post-test, the samples were resin-impregnated, thin-sectioned, and examined by microscopy to define the characteristics of dissolution and precipitation. The test-concurrent X-ray imaging, mass balances, and measurements of permeability, together with the post-test microscopy, were used to define dissolution/precipitation processes, and to constrain process-based models. These models define and quantify key processes of pressure solution, free-face dissolution, and shear-dilation, and the influence of temperature, stress level, and chemistry on the rate of dissolution, its distribution in space and time, and its influence on the mechanical and transport properties of the fracture.

  20. Mouse macrophage polarity and ROCK1 activity depend on RhoA and non-apoptotic Caspase 3.

    PubMed

    Liu, Yianzhu; Minze, Laurie J; Mumma, Lindsay; Li, Xian C; Ghobrial, Rafik M; Kloc, Malgorzata

    2016-02-15

    The macrophages have different subtypes with different functions in immune response and disease. It has been generally accepted that M1 macrophages are responsible for stimulation of immune system and inflammation while M2 macrophages play a role in tissue repair. Irrespective of the type, macrophage functions depend on actin cytoskeleton, which is under the control of small GTPase RhoA pathway and its downstream effector ROCK1. We generated RhoA-deleted macrophages and compared the effect of RhoA deletion on M0, M1 and M2 macrophage phenotype. Our studies showed that, unexpectedly, the RhoA deletion did not eliminate macrophage ROCK1 expression and increased ROCK1 activity. The RhoA deletion effect on macrophage phenotype, structure and polarity was different for each subtype. Moreover, our study indicates that the up-regulation of ROCK1 activity in RhoA-deleted macrophages and macrophage phenotype/polarity are dependent on non-apoptotic Caspase-3 and are sensitive to Caspase-3 inhibition. These novel findings will revise/complement our understanding of RhoA pathway regulation of cell structure and polarity. PMID:26875770

  1. Gusev crater: direction of active winds derived from the Mars Exploration Rover Rock Abrasion Tool

    NASA Astrophysics Data System (ADS)

    Greeley, R.; Gorevan, S.; Thompson, S. D.; Whelley, P.; Squyres, S.; Arvidson, R.

    2004-05-01

    The Mars Exploration Rovers (MERs) are not instrumented to measure winds directly, but might be able to give insight into wind directions using other techniques. The Rock Abrasion Tool (RAT) on the Instrument Deployment Device (IDD) on the Mars rover, Spirit, was used to remove dust and cut into a basaltic rock named Adirondack in Gusev crater on Sol 34 of mission operations. The rock abrasion operation occurred between about 1223 hr and 1518 hr in the afternoon (local solar time) and left a cavity 2.68 mm deep. An image taken after the abrasion operation showed that the rock cuttings were asymmetrically distributed around the cavity and over the rock in a direction suggesting that the cuttings were transported away from the cavity by winds. The distribution pattern (and the inferred wind) is being compared with results from wind tunnel simulations conducted prior to the mission to assess the wind-flow patterns as a function of rock, rover, and IDD positions with respect to the wind. The wind direction inferred from the RAT cuttings are also being compared with wind directions suggested by aeolian bedforms and albedo patterns seen from MER and from orbit, and with directions predicted by a model of the atmosphere for winds at mid-day in Gusev crater.

  2. Mechanisms for trapping and mobilization of residual fluids during capillary-dominated three-phase flow in porous rock

    NASA Astrophysics Data System (ADS)

    Helland, J. O.; Jettestuen, E.

    2016-07-01

    We use a multiphase level set approach to simulate capillary-controlled motions of isolated fluid ganglia surrounded by two other continuous fluids (i.e., double displacements) during three-phase flow on 3-D porous rock geometries. Double displacements and three-phase snap-off mechanisms are closely related. Water snap-off on gas/oil interfaces can initiate double displacements that mobilize isolated oil ganglia in water-wet rock, but it can also terminate ongoing double displacements and trap oil in water. The multiphase level set approach allows for calculating the evolution of disconnected-phase pressure during the motion. In the events of pore filling by double displacement of oil ganglia, and water snap-off on gas/oil interfaces, we find that the local gas/oil capillary pressure drops, while local oil/water capillary pressure increases, by a similar magnitude as observed for the capillary pressure drops during single-pore filling events in dynamic pore-scale experiments of two-phase drainage. We also find that oil ganglia decrease their surface area, and achieve a more compact shape, when the gas/oil interfacial area decreases at the expense of increased oil/water interfacial area during double displacement. By comparison with similar two-phase gas/water simulations, we find that the level of the gas/water capillary pressure curves, including hysteresis loops, are smaller when a mobile, disconnected oil is present, which suggests double displacement of oil is more favorable than direct gas/water displacement. We also present cases in which phase trapping occurred in the three-phase simulations, but not in the corresponding two-phase simulations, supporting the view that more trapping is possible in three-phase flow.

  3. Effect of suction on the mechanical behaviour of iron ore rock

    NASA Astrophysics Data System (ADS)

    Grgic, Dragan; Giot, Richard; Homand, Françoise; Giraud, Albert

    2005-07-01

    The effect of suction on the behaviour of iron ore has been studied from both physical and mechanical points of view. The porosity and the suction phenomena have been analysed using different experimental techniques. Uniaxial compressive tests on partially saturated samples have shown that the suction is responsible for strength and cohesion improvement. Considering the theory of partially saturated porous soils of Coussy and Dangla (Mécanique des sols non saturés (2002 edn). Hermès Science: 2002; 390), we have proposed a constitutive law for partially saturated iron ore. The real increase in the apparent cohesion due to the capillary attraction forces is overestimated if the yield function is written in terms of effective stresses. The effect of the capillary cohesion has been modelled with a function in the expression of the apparent cohesion of the yield function. The effect of suction on the mechanical behaviour has been represented in the effective stresses space and in the total stresses space like the Alonso model (Géotechnique 1990; 40:405-430).

  4. Mechanical Models of Bed-Perpendicular Fractures in Layered Rocks Subjected to Extensional Strain

    NASA Astrophysics Data System (ADS)

    Sanz, P.; Pollard, D. D.; Borja, R. I.

    2010-12-01

    Natural fractures (joints) enhance permeability and therefore are important for the economical production of low-permeability hydrocarbon reservoirs and aquifers. In this work we investigate the formation of bed-perpendicular joints during extension in a stiff brittle layer surrounded by thick softer layers. The quasi-static finite element models consist of three elasto-plastic layers with frictional bedding interfaces and the middle layer contains layer-perpendicular fractures that can accommodate opening at the bedding surface accompanied by interface sliding. The upper and lower boundaries are subject to normal tractions appropriate for the depth of burial. Lateral boundaries are displaced horizontally to represent the extensional tectonic regime. We use an interface model that captures the most important mechanical features during sliding of bedding interfaces and opening of joints: unilateral contact, elastic and plastic relative deformation, tensile strength, cohesion, frictional sliding, and non-associative plastic flow. The constitutive law extends the Coulomb slip criterion to the tensile regime to capture opening of fractures in a quasi-brittle manner. The finite element implementation employs a penalty scheme to impose the contact constraints along the interfaces. The numerical simulations show the effects of mechanical properties of layers and interfaces in the development and spacing of bed-perpendicular joints. We evaluate the concepts of fracture saturation and sequential infilling, and the relationship between joint spacing and layer thickness in the context of the new modeling capabilities.

  5. Time-dependent Deformation in Porous Rocks Driven by Chemo-mechanical Coupling

    NASA Astrophysics Data System (ADS)

    Meredith, P. G.; Brantut, N.; Heap, M. J.; Baud, P.

    2015-12-01

    We report results from triaxial deformation of porous sandstone and limestone conducted to determine the time-dependency of deformation. Experiments were run on water-saturated samples under constant differential stress (creep) conditions. In sandstone, the deformation is entirely brittle for all levels of stress and for all resulting strain rates. The strain rate during creep is very stress sensitive, with a change of only 20 MPa in differential stress producing three orders of magnitude change in strain rate. Failure occurs by localized shear faulting after an extended period of dilatant microcracking, as evidenced by the output of acoustic emissions. By contrast, the behaviour of limestone is more complex. At low effective pressure, the creep behavior is brittle and characterised by the same features as observed for sandstone; a decelerating phase of creep, followed by an inflection and then an accelerating creep phase leading to macroscopic failure. Similarly, only a small amount of inelastic strain is accommodated before failure, and P wave speeds measured throughout deformation decrease continuously, indicating a continuous increase in dilatant crack damage. At higher effective pressure, brittle creep still occurs, but the details of the time-dependent deformation behavior are quite different. First, the total amount of accumulated creep strain increases dramatically with decreasing strain rate, and no localized failure occurs even at these higher strains. Second, the rate of decrease in P wave speeds during deformation decreases with decreasing strain rate; indicating that less damage is accumulated per unit strain when the strain rate is lower. Third, complementary strain rate stepping experiments indicate that the deformation becomes more compactant at lower strain rates. Taken together, these observations suggest that rate-dependent compactive deformation mechanisms compete with dilatant subcritical crack growth during creep in limestone under low

  6. From rock fracture to plate tectonics. Evidence of non extensive statistical mechanics in Earth physics, A review

    NASA Astrophysics Data System (ADS)

    Vallianatos, F.

    2012-04-01

    The non-extensive statistical mechanics pioneered by the Tsallis group offers a consistent theoretical framework, based on a generalization of entropy, to analyze the behavior of systems with fractal or multi-fractal distribution of their elements. Such systems where long-range interactions or intermittency are important, lead to power law behavior. The question of whether earth systems are described by non-extensive statistical physics, even at the phenomenological level (i.e., without specifying any underlying model), represents a challenge. This is the problem we review here. Our aim is not to present a precise model, but rather to emphasize in simple arguments of physical plausibility. Examples supporting the non-additive behavior of earth system, from rocks fracture (e.g., acoustic emissions) to geodynamic (e.g., plate tectonics, global seismicity) scale are presented. Acknowledgments. This work was partly supported by the THALES Program of the Ministry of Education of Greece and the European Union in the framework of the project entitled "Integrated understanding of Seismicity, using innovative Methodologies of Fracture mechanics along with Earthquake and non extensive statistical physics - Application to the geodynamic system of the Hellenic Arc. SEISMO FEAR HELLARC".

  7. Site study plan for non-routine laboratory rock mechanics, Deaf Smith County Site, Texas: Revision 1

    SciTech Connect

    Not Available

    1987-12-01

    This Site Study Plan describes the non-routine rock mechanics and thermal properties laboratory testing program planned for the characterization of site-specific geologic materials for the Deaf Smith County site, Texas. The study design provides for measurements of index, mechanical, thermomechanical, thermal and special properties for the host salt, and where appropriate, for nonhost lithologies. The types of tests which will be conducted are constant stress (creep) tests, constant strain (stress relaxation) tests, constant strain-rate tests, constant stress-rate tests, cyclic loading tests, hollow cylinder tests, uniaxial and triaxial compression tests, direct tension tests, indirect (triaxial) shear tests, thermal property determinations (conductivity, specific heat, expansivity, and diffusivity), fracture healing tests, thermal decrepitation tests, moisture content determinations, and petrographic and micromechanics analyses. Tests will be conducted at confining pressures up to 30 MPa and temperatures up to 300/degree/C. These data are used to construct mathematical models for the phenomenology of salt deformation. The models are then used in finite-element codes to predict repository response. A tentative testing schedule and milestone log are given. The duration of the testing program is expected to be approximately 5 years. 44 refs., 13 figs., 13 tabs.

  8. Quasi-static rock mechanics data for rocksalt from three Strategic Petroleum Reserve domes

    SciTech Connect

    Price, R.H.; Wawersik, W.R.; Hannum, D.W.; Zirzow, J.A.

    1981-12-01

    Triaxial compression and extension experiments have been run on rocksalt samples from three Strategic Petroleum Reserve (SPR) domes. Seventeen quasi-static tests were loaded at mean stress rates of .66 to 1.04 psi/sec (4.5 to 7.2 kPa/sec), confining pressures of 14.5 to 2000 psi (0.1 to 13.8 MPa) and temperatures of 22 to 100/sup 0/C. Eleven of the test specimens were from Bryan Mound, Texas, and three each were from Bayou Choctaw, Louisiana, and West Hackberry, Louisiana. In general, the resulting mechanical data from the three domes are similar, and they are consistent with previously published data. Ultimate sample strengths are directly related to confining pressure (least principal stress) and indirectly related to temperature, while ductility increases with both pressure and temperature.

  9. Deformation mechanisms of antigorite serpentinite at subduction zone conditions determined from experimentally and naturally deformed rocks

    NASA Astrophysics Data System (ADS)

    Auzende, Anne-Line; Escartin, Javier; Walte, Nicolas P.; Guillot, Stéphane; Hirth, Greg; Frost, Daniel J.

    2015-02-01

    We performed deformation-DIA experiments on antigorite serpentinite at pressures of 1-3.5 GPa and temperatures of between 400 and 650 °C, bracketing the stability of antigorite under subduction zone conditions. For each set of pressure-temperature (P-T) conditions, we conducted two runs at strain rates of 5 ×10-5 and 1 ×10-4 s-1. We complemented our study with a sample deformed in a Griggs-type apparatus at 1 GPa and 400 °C (Chernak and Hirth, 2010), and with natural samples from Cuba and the Alps deformed under blueschist/eclogitic conditions. Optical and transmission electron microscopies were used for microstructural characterization and determination of deformation mechanisms. Our observations on experimentally deformed antigorite prior to breakdown show that deformation is dominated by cataclastic flow with observable but minor contribution of plastic deformation (microkinking and (001) gliding mainly expressed by stacking disorder mainly). In contrast, in naturally deformed samples, plastic deformation structures are dominant (stacking disorder, kinking, pressure solution), with minor but also perceptible contribution of brittle deformation. When dehydration occurs in experiments, plasticity increases and is coupled to local embrittlement that we attribute to antigorite dehydration. In dehydrating samples collected in the Alps, embrittlement is also observed suggesting that dehydration may contribute to intermediate-depth seismicity. Our results thus show that semibrittle deformation operates within and above the stability field of antigorite. However, the plastic deformation recorded by naturally deformed samples was likely acquired at low strain rates. We also document that the corrugated structure of antigorite controls the strain accommodation mechanisms under subduction conditions, with preferred inter- and intra-grain cracking along (001) and gliding along both a and b. We also show that antigorite rheology in subduction zones is partly controlled

  10. Lithological Controls on 3D Fold Geometry in Mechanically Layered Rocks

    NASA Astrophysics Data System (ADS)

    Pearce, M. A.; Jones, R. R.; Rock, G.

    2010-12-01

    Folding and thrusting are key processes in accommodating shortening in evolving orogens. In the outer parts of mountain belts, the combination of tectonism and sedimentation often leads to viable petroleum systems where folds trap migrating hydrocarbons. A key aspect of the success of these traps is the 3D fold geometry, which must prevent up-plunge hydrocarbon escape. Fold shapes in a multilayered system are governed by the fold mechanism, and the rheology of the layers. In sedimentary environments where laterally heterogeneous thicknesses of interbedded carbonates, clastics, and evaporates may be present, predicting the sub-surface geometry of structures is challenging. We present field data combined with satellite and seismic interpretation from the Zagros fold and thrust belt of SE Turkey where Neogene shortening has affected an Ordovician to Miocene aged sedimentary pile comprising shales, sandstones, carbonates and minor evaporites. Miocene carbonates capping the sequence allow 3D fold geometries to be ascertained at surface with a high degree of confidence, whilst the underlying ~1km of shales and mudstones allows folds to develop that depart substantially from standard “text-book” geometries. Beneath these incompetent units a further 1.7km of carbonates and clastics overlie Ordovician shales up to 1.5km thick. In relatively low strain sections asymmetric, angular, kink-like folds form long wavelength structures and thrust faults rarely reach the surface. With increasing strain, the wavelength/amplitude ratio decreases and thrusts cut through the fold limbs. Folds are interpreted to detach above a thick sequence of Ordovician shales. They originate by buckling of the competent units within the two weaker horizons, with initial perturbations probably provided by sedimentary heterogeneities. As the folds amplify, thrusts form in the more competent units above the shale. These then propagate upwards with fold amplification dominated by fault-tip

  11. Immobilization and phytotoxicity of Pb in contaminated soil amended with γ-polyglutamic acid, phosphate rock, and γ-polyglutamic acid-activated phosphate rock.

    PubMed

    Zhu, Jun; Cai, Zhijian; Su, Xiaojuan; Fu, Qingling; Liu, Yonghong; Huang, Qiaoyun; Violante, Antonio; Hu, Hongqing

    2015-02-01

    Pot experiments were conducted to investigate the effects of γ-polyglutamic acid (γ-PGA), phosphate rock (PR), and γ-PGA-activated PR (γ-PGA-PR) on the immobilization and phytotoxicity of Pb in a contaminated soil. The proportion of residual Pb (Re-Pb) in soil was reduced by the addition of γ-PGA but was increased by the application of PR and γ-PGA-PR. The addition of γ-PGA in soil improved the accumulation of Pb in pak choi and decreased the growth of pak choi, suggesting the intensification of Pb phytotoxicity to pak choi. However, opposite effects of PR and γ-PGA-PR on the phytotoxicity of Pb to pak choi in soil were observed. Moreover, in the examined range, γ-PGA-PR activated by a higher amount of γ-PGA resulted in a greater proportion of Re-Pb in soil and weaker phytotoxicity of Pb to pak choi. The predominance of γ-PGA-PR in relieving the phytotoxicity of Pb was ascribed mainly to the increase of soil pH and available phosphate after the amendment, which could facilitate the precipitation of Pb in soil and provide pak choi with more phosphorus nutrient.

  12. Fault-related clay authigenesis along the Moab Fault: Implications for calculations of fault rock composition and mechanical and hydrologic fault zone properties

    USGS Publications Warehouse

    Solum, J.G.; Davatzes, N.C.; Lockner, D.A.

    2010-01-01

    The presence of clays in fault rocks influences both the mechanical and hydrologic properties of clay-bearing faults, and therefore it is critical to understand the origin of clays in fault rocks and their distributions is of great importance for defining fundamental properties of faults in the shallow crust. Field mapping shows that layers of clay gouge and shale smear are common along the Moab Fault, from exposures with throws ranging from 10 to ???1000 m. Elemental analyses of four locations along the Moab Fault show that fault rocks are enriched in clays at R191 and Bartlett Wash, but that this clay enrichment occurred at different times and was associated with different fluids. Fault rocks at Corral and Courthouse Canyons show little difference in elemental composition from adjacent protolith, suggesting that formation of fault rocks at those locations is governed by mechanical processes. Friction tests show that these authigenic clays result in fault zone weakening, and potentially influence the style of failure along the fault (seismogenic vs. aseismic) and potentially influence the amount of fluid loss associated with coseismic dilation. Scanning electron microscopy shows that authigenesis promotes that continuity of slip surfaces, thereby enhancing seal capacity. The occurrence of the authigenesis, and its influence on the sealing properties of faults, highlights the importance of determining the processes that control this phenomenon. ?? 2010 Elsevier Ltd.

  13. Fault-related clay authigenesis along the Moab Fault: Implications for calculations of fault rock composition and mechanical and hydrologic fault zone properties

    NASA Astrophysics Data System (ADS)

    Solum, John G.; Davatzes, Nicholas C.; Lockner, David A.

    2010-12-01

    The presence of clays in fault rocks influences both the mechanical and hydrologic properties of clay-bearing faults, and therefore it is critical to understand the origin of clays in fault rocks and their distributions is of great importance for defining fundamental properties of faults in the shallow crust. Field mapping shows that layers of clay gouge and shale smear are common along the Moab Fault, from exposures with throws ranging from 10 to ˜1000 m. Elemental analyses of four locations along the Moab Fault show that fault rocks are enriched in clays at R191 and Bartlett Wash, but that this clay enrichment occurred at different times and was associated with different fluids. Fault rocks at Corral and Courthouse Canyons show little difference in elemental composition from adjacent protolith, suggesting that formation of fault rocks at those locations is governed by mechanical processes. Friction tests show that these authigenic clays result in fault zone weakening, and potentially influence the style of failure along the fault (seismogenic vs. aseismic) and potentially influence the amount of fluid loss associated with coseismic dilation. Scanning electron microscopy shows that authigenesis promotes that continuity of slip surfaces, thereby enhancing seal capacity. The occurrence of the authigenesis, and its influence on the sealing properties of faults, highlights the importance of determining the processes that control this phenomenon.

  14. Deformation and softening mechanism in naturally deformed rocks at the brittle-ductile transition zone in upper crust: pervasive micro-faulting accommodated by pressure solution of quartz

    NASA Astrophysics Data System (ADS)

    Takeshita, T.; El-Fakharani, A.

    2011-12-01

    Although the conventional two-mechanism strength profile of upper crustal rheology has been applied for a long time to geodynamical problems, the high differential stresses as much as a few hundreds of MPa have never been reported from geophysical observations such as those inferred from heat flow along active faults and stress drop during earthquakes. These facts suggest that there must be some softening mechanisms around the brittle-ductile transition zone in upper crust, where inland earthquakes most frequently occur. In order to unravel softening mechanisms in these regions, we have been examining natural microstructures in exhumed metamorphic rocks, which experienced pervasive deformation at brittle-ductile conditions (T=c. 300 oC). The Sambagawa metamorphic rocks experienced localized deformation under brittle-ductile transition conditions at D2 phase during exhumation. At outcrop scales, low-angle normal faults were pervasively developed with a dominant top-to-the-NNW movement recorded in quartz slickenfibre. In quartz schist deformed at D2 phase, shear bands coated by phengite were pervasively developed. We have extensively studied quartz c-axis fabrics and microstructures in the micro-faulted quartz schist. In quartz lenses surrounded by D2 shear bands, quartz microstructures and c-axis fabrics formed at D1 phase indicative of dislocation creep were well preserved. However, in the matrix (i.e. domains outside the lenses), quartz c-axis fabrics became weakened, and in some cases, became completely random. We have analyzed the degree of undulation of recrystallized quartz grain boundaries using the index called normalized perimeter of grains to that of the equivalent ellipse obtained with the NIH image, and compared the degree among different quartz schist samples and domains in the same sample. As a result, it has been found out that there is a nice positive correlation between the degree of grain boundary undulation and c-axis fabric intensity in quartz

  15. Training and Research on Probabilistic Hydro-Thermo-Mechanical Modeling of Carbon Dioxide Geological Sequestration in Fractured Porous Rocks

    SciTech Connect

    Gutierrez, Marte

    2013-05-31

    Colorado School of Mines conducted research and training in the development and validation of an advanced CO{sub 2} GS (Geological Sequestration) probabilistic simulation and risk assessment model. CO{sub 2} GS simulation and risk assessment is used to develop advanced numerical simulation models of the subsurface to forecast CO2 behavior and transport; optimize site operational practices; ensure site safety; and refine site monitoring, verification, and accounting efforts. As simulation models are refined with new data, the uncertainty surrounding the identified risks decrease, thereby providing more accurate risk assessment. The models considered the full coupling of multiple physical processes (geomechanical and fluid flow) and describe the effects of stochastic hydro-mechanical (H-M) parameters on the modeling of CO{sub 2} flow and transport in fractured porous rocks. Graduate students were involved in the development and validation of the model that can be used to predict the fate, movement, and storage of CO{sub 2} in subsurface formations, and to evaluate the risk of potential leakage to the atmosphere and underground aquifers. The main major contributions from the project include the development of: 1) an improved procedure to rigorously couple the simulations of hydro-thermomechanical (H-M) processes involved in CO{sub 2} GS; 2) models for the hydro-mechanical behavior of fractured porous rocks with random fracture patterns; and 3) probabilistic methods to account for the effects of stochastic fluid flow and geomechanical properties on flow, transport, storage and leakage associated with CO{sub 2} GS. The research project provided the means to educate and train graduate students in the science and technology of CO{sub 2} GS, with a focus on geologic storage. Specifically, the training included the investigation of an advanced CO{sub 2} GS simulation and risk assessment model that can be used to predict the fate, movement, and storage of CO{sub 2} in

  16. Active airborne infrared laser system for identification of surface rock and minerals

    NASA Technical Reports Server (NTRS)

    Kahle, A. B.; Shumate, M. S.; Nash, D. B.

    1984-01-01

    Emissivity and reflectivity in the thermal infrared spectral region (8-13 microns) may be used to discriminate among rocks and minerals. Although considerable success has been achieved in remote sensing classification of rock types based on emissivity measurements made with NASA's Thermal Infreared Multispectral Scanner (TIMS), classification based on reflectivity offers several advantages: much narrower bandwidths are used, higher signal to noise ratios are possible, and measurements are little affected by surface temperature. As a demonstration, an airborne CO2 laser instrument was flown along the margin of Death Valley, California. Measurements of spectral reflectance collected with this device were compared with emissivity measurements made with the TIMS. Data from either instrument provided the means for recognizing boundaries between geologic units including different rock types and fan surfaces of different ages.

  17. Talking Rocks.

    ERIC Educational Resources Information Center

    Rice, Dale; Corley, Brenda

    1987-01-01

    Discusses some of the ways that rocks can be used to enhance children's creativity and their interest in science. Suggests the creation of a dramatic production involving rocks. Includes basic information on sedimentary, igneous, and metamorphic rocks. (TW)

  18. Using earthquake-triggered landslides as a hillslope-scale shear strength test: Insights into rock strength properties at geomorphically relevant spatial scales in high-relief, tectonically active settings

    NASA Astrophysics Data System (ADS)

    Gallen, Sean; Clark, Marin; Godt, Jonathan; Lowe, Katherine

    2016-04-01

    obtained using typical laboratory shear strength measurements on intact rock samples. Furthermore, the near-surface material strength is similar between the study areas despite differences in tectonic, climatic, and lithologic conditions. Variations in near-surface strength within each setting appear to be more strongly associated with factors contributing to the weakening rock through chemical or physical weathering, such as mean annual precipitation and distance to active faults (a proxy for rock shattering intensity), rather than intrinsic lithologic properties. We hypothesize that the shattering of rock through long-term permanent strain accumulation and by repeated earthquakes is an important mechanism that can explain low rock strength values among the different study sites and the spatial pattern of rock strength within each location. These findings emphasize the potential role of factors other than lithology in controlling the spatial distribution of near-surface rock strength in high-relief, tectonically active settings, which has important implications for understanding the evolution of landscapes, interpreting tectonic and climatic signals from topography, critical zone processes, and natural hazard assessment.

  19. Microbial populations and activities in the rhizoplane of rock-weathering desert plants. I. Root colonization and weathering of igneous rocks.

    PubMed

    Puente, M E; Bashan, Y; Li, C Y; Lebsky, V K

    2004-09-01

    Dense layers of bacteria and fungi in the rhizoplane of three species of cactus (Pachycereus pringlei, Stenocereus thurberi, Opuntia cholla) and a wild fig tree (Ficus palmeri) growing in rocks devoid of soil were revealed by bright-field and fluorescence microscopy and field emission scanning electron microscopy. These desert plants are responsible for rock weathering in an ancient lava flow at La Purisima-San Isidro and in sedimentary rock in the Sierra de La Paz, both in Baja California Sur, Mexico. The dominant bacterial groups colonizing the rhizoplane were fluorescent pseudomonads and bacilli. Seven of these bacterial species were identified by the 16S rRNA molecular method. Unidentified fungal and actimomycete species were also present. Some of the root-colonizing microorganisms fixed in vitro N(2), produced volatile and non-volatile organic acids that subsequently reduced the pH of the rock medium in which the bacteria grew, and significantly dissolved insoluble phosphates, extrusive igneous rock, marble, and limestone. The bacteria were able to release significant amounts of useful minerals, such as P, K, Mg, Mn, Fe, Cu, and Zn from the rocks and were thermo-tolerant, halo-tolerant, and drought-tolerant. The microbial community survived in the rhizoplane of cacti during the annual 10-month dry season. This study indicates that rhizoplane bacteria on cacti roots in rock may be involved in chemical weathering in hot, subtropical deserts.

  20. Microbial populations and activities in the rhizoplane of rock-weathering desert plants. I. Root colonization and weathering of igneous rocks.

    PubMed

    Puente, M E; Bashan, Y; Li, C Y; Lebsky, V K

    2004-09-01

    Dense layers of bacteria and fungi in the rhizoplane of three species of cactus (Pachycereus pringlei, Stenocereus thurberi, Opuntia cholla) and a wild fig tree (Ficus palmeri) growing in rocks devoid of soil were revealed by bright-field and fluorescence microscopy and field emission scanning electron microscopy. These desert plants are responsible for rock weathering in an ancient lava flow at La Purisima-San Isidro and in sedimentary rock in the Sierra de La Paz, both in Baja California Sur, Mexico. The dominant bacterial groups colonizing the rhizoplane were fluorescent pseudomonads and bacilli. Seven of these bacterial species were identified by the 16S rRNA molecular method. Unidentified fungal and actimomycete species were also present. Some of the root-colonizing microorganisms fixed in vitro N(2), produced volatile and non-volatile organic acids that subsequently reduced the pH of the rock medium in which the bacteria grew, and significantly dissolved insoluble phosphates, extrusive igneous rock, marble, and limestone. The bacteria were able to release significant amounts of useful minerals, such as P, K, Mg, Mn, Fe, Cu, and Zn from the rocks and were thermo-tolerant, halo-tolerant, and drought-tolerant. The microbial community survived in the rhizoplane of cacti during the annual 10-month dry season. This study indicates that rhizoplane bacteria on cacti roots in rock may be involved in chemical weathering in hot, subtropical deserts. PMID:15375735

  1. Assessment of Hydro-Mechanical Behavior of a Granite Rock Mass for a Pilot Underground Crude Oil Storage Facility in China

    NASA Astrophysics Data System (ADS)

    Wang, Zhechao; Li, Shucai; Qiao, Liping

    2015-11-01

    The hydro-mechanical behavior of a pilot underground crude oil storage facility in a granite host rock in China was analyzed using the finite element method (FEM). Characterization of hydro-mechanical behavior of the rock mass was performed using laboratory test, field monitoring, back analysis of field measurements and permeability tests. FEM numerical analyses were used to assess the hydro-mechanical behavior of the granite to study several design and construction issues. The containment properties of the storage facility were investigated without and with the water curtain system. Results showed that the stored oil would leak into rock mass if a water curtain system is not provided, whereas the containment property of the facility will be maintained when a water curtain system is in place. On the influence of cavern excavation sequence, it was indicated that the excavation of the caverns from left to right is a better choice than right to left for the containment property of the facility. On the influence of permeable condition, it was found that the extent of plastic zones, horizontal convergence and crown settlement under permeable condition are lower than those under impermeable condition due to the different stress paths in the rock mass experienced during excavation.

  2. 76 FR 28460 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Rock Burst...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-17

    ...; Rock Burst Control Plan--Pertains to Underground Metal and Nonmetal Mines ACTION: Notice. SUMMARY: The Department of Labor (DOL) is submitting the Mine Safety and Health Administration (MSHA) sponsored... Mines,'' to the Office of Management and Budget (OMB) for review and approval for continued use...

  3. Thermal-chemical-mechanical feedback during fluid-rock interactions: Implications for chemical transport and scales of equilibria in the crust

    SciTech Connect

    Dutrow, Barbara

    2008-08-13

    Our research evaluates the hypothesis that feedback amongst thermal-chemical-mechanical processes operative in fluid-rock systems alters the fluid flow dynamics of the system which, in turn, affects chemical transport and temporal and spatial scales of equilibria, thus impacting the resultant mineral textural development of rocks. Our methods include computational experimentation and detailed analyses of fluid-infiltrated rocks from well-characterized terranes. This work focuses on metamorphic rocks and hydrothermal systems where minerals and their textures are utilized to evaluate pressure (P), temperature (T), and time (t) paths in the evolution of mountain belts and ore deposits, and to interpret tectonic events and the timing of these events. Our work on coupled processes also extends to other areas where subsurface flow and transport in porous media have consequences such as oil and gas movement, geothermal system development, transport of contaminants, nuclear waste disposal, and other systems rich in fluid-rock reactions. Fluid-rock systems are widespread in the geologic record. Correctly deciphering the products resulting from such systems is important to interpreting a number of geologic phenomena. These systems are characterized by complex interactions involving time-dependent, non-linear processes in heterogeneous materials. While many of these interactions have been studied in isolation, they are more appropriately analyzed in the context of a system with feedback. When one process impacts another process, time and space scales as well as the overall outcome of the interaction can be dramatically altered. Our goals to test this hypothesis are: to develop and incorporate algorithms into our 3D heat and mass transport code to allow the effects of feedback to be investigated numerically, to analyze fluid infiltrated rocks from a variety of terranes at differing P-T conditions, to identify subtle features of the infiltration of fluids and/or feedback, and

  4. Mechanisms of Specificity for Hox Factor Activity

    PubMed Central

    Zandvakili, Arya; Gebelein, Brian

    2016-01-01

    Metazoans encode clusters of paralogous Hox genes that are critical for proper development of the body plan. However, there are a number of unresolved issues regarding how paralogous Hox factors achieve specificity to control distinct cell fates. First, how do Hox paralogs, which have very similar DNA binding preferences in vitro, drive different transcriptional programs in vivo? Second, the number of potential Hox binding sites within the genome is vast compared to the number of sites bound. Hence, what determines where in the genome Hox factors bind? Third, what determines whether a Hox factor will activate or repress a specific target gene? Here, we review the current evidence that is beginning to shed light onto these questions. In particular, we highlight how cooperative interactions with other transcription factors (especially PBC and HMP proteins) and the sequences of cis-regulatory modules provide a basis for the mechanisms of Hox specificity. We conclude by integrating a number of the concepts described throughout the review in a case study of a highly interrogated Drosophila cis-regulatory module named “The Distal-less Conserved Regulatory Element” (DCRE). PMID:27583210

  5. The influence of rigid second phases on the mechanical evolution of calcite rocks, with implications for strain localization

    NASA Astrophysics Data System (ADS)

    Austin, N.; Evans, B.; Dresen, G.; Rybaki, E.

    2007-12-01

    Interactions between rheologically distinct phases may strongly influence both the microstructure and mechanical properties of shear zones, thus playing a key role in the development or inhibition of localized deformation. However, experimental, theoretical, and field observations have produced conflicting suggestions that either A) rigid phases may pin the grain-size of a dominant weak phase, driving deformation into the diffusion creep regime, and promoting weakening, or B) rigid phases may be load supporting, thus increasing the strength of the aggregate. To investigate the influence of chemically inert, rigid second phases on the strength, microstructural evolution, and dominant deformation mechanism of calcite rocks, we have performed conventional triaxial experiments (MIT), and high strain torsion experiments (GFZ-Potsdam) on synthetic calcite aggregates with no second phases added, 1% (by vol.) 4 μm carbon spheres, 10% (by vol.) 4 μm carbon spheres, and 10% (by vol.) 5 μm carbon splinters. Glassy carbon spheres and splinters were chosen as they are chemically inert under the experimental conditions, are mechanically strong compared to the calcite, and can be obtained with a narrow grain size distribution. Prior to deformation, samples were hot isostatically pressed (HIPed) at 1023K and 300 MPa, to control the starting grain-size. The presence of carbon spheres or splinters has a dramatic influence on the grain-size after HIPing: samples with 10% spheres or splinters have an initial grain-size that is a factor of three finer than pure samples after 2.25 hrs while samples with 1% spheres are ~1.5 times finer than pure samples, again after 2.25 hrs. Deformation experiments were performed at 1023K, at a confining pressure of 300 MPa (trixial) or 400 MPa (torsion), at constant equivalent strain rates between 5e-6 s-1 and 1e-3 s-1. In both triaxial compression and torsion experiments, samples containing 10% splinters are stronger than pure samples and those

  6. Inhibition of RHO-ROCK signaling enhances ICM and suppresses TE characteristics through activation of Hippo signaling in the mouse blastocyst

    PubMed Central

    Kono, Kanako; Tamashiro, Dana Ann A.; Alarcon, Vernadeth B.

    2014-01-01

    Specification of the trophectoderm (TE) and inner cell mass (ICM) lineages in the mouse blastocyst correlates with cell position, as TE derives from outer cells whereas ICM from inner cells. Differences in position are reflected by cell polarization and Hippo signaling. Only in outer cells, the apical-basal cell polarity is established, and Hippo signaling is inhibited in such a manner that LATS1 and 2 (LATS1/2) kinases are prevented from phosphorylating YAP, a key transcriptional co-activator of the TE-specifying gene Cdx2. However, the molecular mechanisms that regulate these events are not fully understood. Here, we showed that inhibition of RHO-ROCK signaling enhances ICM and suppresses TE characteristics through activation of Hippo signaling and disruption of apical-basal polarity. Embryos treated with ROCK inhibitor Y-27632 exhibited elevated expression of ICM marker NANOG and reduced expression of CDX2 at the blastocyst stage. Y-27632-treated embryos failed to accumulate YAP in the nucleus, although it was rescued by concomitant inhibition of LATS1/2. Segregation between apical and basal polarity regulators, namely PARD6B, PRKCZ, SCRIB, and LLGL1, was dampened by Y-27632 treatment, whereas some of the polarization events at the late 8-cell stage such as compaction and apical localization of p-ERM and tyrosinated tubulin occurred normally. Similar abnormalities of Hippo signaling and apical-basal polarization were also observed in embryos that were treated with RHO GTPases inhibitor. These results suggest that RHO-ROCK signaling plays an essential role in regulating Hippo signaling and cell polarization to enable proper specification of the ICM and TE lineages. PMID:24997360

  7. Displaced rocks, strong motion, and the mechanics of shallow faulting associated with the 1999 Hector Mine, California, earthquake

    USGS Publications Warehouse

    Michael, A.J.; Ross, S.L.; Stenner, H.D.

    2002-01-01

    The paucity of strong-motion stations near the 1999 Hector Mine earthquake makes it impossible to make instrumental studies of key questions about near-fault strong-motion patterns associated with this event. However, observations of displaced rocks allow a qualitative investigation of these problems. By observing the slope of the desert surface and the frictional coefficient between these rocks and the desert surface, we estimate the minimum horizontal acceleration needed to displace the rocks. Combining this information with observations of how many rocks were displaced in different areas near the fault, we infer the level of shaking. Given current empirical shaking attenuation relationships, the number of rocks that moved is slightly lower than expected; this implies that slightly lower than expected shaking occurred during the Hector Mine earthquake. Perhaps more importantly, stretches of the fault with 4 m of total displacement at the surface displaced few nearby rocks on 15?? slopes, suggesting that the horizontal accelerations were below 0.2g within meters of the fault scarp. This low level of shaking suggests that the shallow parts of this rupture did not produce strong accelerations. Finally, we did not observe an increased incidence of displaced rocks along the fault zone itself. This suggests that, despite observations of fault-zone-trapped waves generated by aftershocks of the Hector Mine earthquake, such waves were not an important factor in controlling peak ground acceleration during the mainshock.

  8. Determination of trace halogens in rock samples by radiochemical neutron activation analysis coupled with the k0-standardization method.

    PubMed

    Ozaki, Hiromasa; Ebihara, Mitsuru

    2007-02-01

    Radiochemical neutron activation analysis coupled with the k0-standardization method (k0-RNAA method) was applied to silicate rock samples for the simultaneous determination of trace halogens (Cl, Br and I). Analytical results obtained by the k0-RNAA method for geological standard rocks and meteorite samples agreed with those determined by the conventional comparison method conducted in the same set of experiments, suggesting that the k0-RNAA method is as reliable as the conventional method. Our data for these samples are in good agreement with their literature values except for rare cases. Detection limits calculated under the present experimental condition are sufficiently low for Cl and Br but not for I for typical geologic and meteoritic samples. The k0-RNAA method coupled with longer neutron-irradiation is expected to yield satisfactorily low detection limits for halogens including I in these samples.

  9. Impact of solid second phases on deformation mechanisms of naturally deformed salt rocks (Kuh-e-Namak, Dashti, Iran) and rheological stratification of the Hormuz Salt Formation

    NASA Astrophysics Data System (ADS)

    Závada, P.; Desbois, G.; Urai, J. L.; Schulmann, K.; Rahmati, M.; Lexa, O.; Wollenberg, U.

    2015-05-01

    Viscosity contrasts displayed in flow structures of a mountain namakier (Kuh-e-Namak - Dashti), between 'weak' second phase bearing rock salt and 'strong' pure rock salt types are studied for deformation mechanisms using detailed quantitative microstructural study. While the solid inclusions rich ("dirty") rock salts contain disaggregated siltstone and dolomite interlayers, "clean" salts reveal microscopic hematite and remnants of abundant fluid inclusions in non-recrystallized cores of porphyroclasts. Although the flow in both, the recrystallized "dirty" and "clean" salt types is accommodated by combined mechanisms of pressure-solution creep (PS), grain boundary sliding (GBS), transgranular microcracking and dislocation creep accommodated grain boundary migration (GBM), their viscosity contrasts observed in the field outcrops are explained by: 1) enhanced ductility of "dirty" salts due to increased diffusion rates along the solid inclusion-halite contacts than along halite-halite contacts, and 2) slow rates of intergranular diffusion due to dissolved iron and inhibited dislocation creep due to hematite inclusions for "clean" salt types Rheological contrasts inferred by microstructural analysis between both salt rock classes apply in general for the "dirty" salt forming Lower Hormuz and the "clean" salt forming the Upper Hormuz of the Hormuz Formation and imply strain rate gradients or decoupling along horizons of mobilized salt types of different composition and microstructure.

  10. Prolonged magmatic activity on Mars inferred from the detection of felsic rocks

    USGS Publications Warehouse

    Wray, James J.; Hansen, Sarah T.; Dufek, Josef; Swayze, Scott L.; Murchie, Scott L.; Seelos, Frank P.; Skok, John R.; Irwin, Rossman P.; Ghiorso, Mark S.

    2013-01-01

    Rocks dominated by the silicate minerals quartz and feldspar are abundant in Earth’s upper continental crust. Yet felsic rocks have not been widely identified on Mars, a planet that seems to lack plate tectonics and the associated magmatic processes that can produce evolved siliceous melts on Earth. If Mars once had a feldspar-rich crust that crystallized from an early magma ocean such as that on the Moon, erosion, sedimentation and volcanism have erased any clear surface evidence for widespread felsic materials. Here we report near-infrared spectral evidence from the Compact Reconnaissance Imaging Spectrometer for Mars onboard the Mars Reconnaissance Orbiter for felsic rocks in three geographically disparate locations on Mars. Spectral characteristics resemble those of feldspar-rich lunar anorthosites, but are accompanied by secondary alteration products (clay minerals). Thermodynamic phase equilibrium calculations demonstrate that fractional crystallization of magma compositionally similar to volcanic flows near one of the detection sites can yield residual melts with compositions consistent with our observations. In addition to an origin by significant magma evolution, the presence of felsic materials could also be explained by feldspar enrichment by fluvial weathering processes. Our finding of felsic materials in several locations on Mars suggests that similar observations by the Curiosity rover in Gale crater may be more widely applicable across the planet.

  11. Dynamics of Fragmentation: Developing a non-Equilibrium Mechanism for Impact-loading tests on Rock Materials

    NASA Astrophysics Data System (ADS)

    Ghaffari, H.; Griffith, W. A.; Barber, T. J.

    2015-12-01

    Formation of fragments as the result of dynamic processes associated with impulsive loads has been the subject of numerous studies ranging from shaped-charge jet break up and rock blasting to bolide impacts, and, more recently, earthquake rupture. The dynamic strength of solids is varies as a strong function of loading rate, and, in completely failed solids, the characteristic size of fragments is related to the loading dynamics. In this study, we present some novel results using fragmentation of an "order" parameter in an isotropic body, while we use a non-equilibrium thermodynamic formulation to infer characteristics of the fragments. The order parameter is related to general rigidity of the system and is investigated in 3D space including amplitude and phase modes. To this end, we use the idea of the formation of topological defects in the course of rapid pressure changes and show that a power-law scaling describes transient strength versus inverse of the stress-ramp time. Furthermore, we illustrate that the coefficient of this power-law is deeply connected to relaxation (healing) time of the body. In addition, we show that dynamic polarization patterns of the dynamic cracks are analogous to the transition from ferrimagnets to paramagnets, providing insight to the dynamics of microscopic-scale catastrophic failure. This connection helps us to use the Kibble-Zurek mechanism (KZM) to infer the size of fragments from loading rate when considering a linear loading ramp. The idea behind the KZM is to compare the relaxation time (or healing time of the system in equilibrium) with the timescale of change of the control parameter (ɛ). In addition, we discuss a case where inherent defects are present prior to the impulse load and discuss the effect of impurities on the scaling coefficients. To support our approach, we use the results of fast-loading experiments on Westerly Granite supported by recording multiple acoustic emissions.

  12. Basal sliding in ice streams as seen through the lens of rock mechanics: an experimental study of ice-on-rock friction

    NASA Astrophysics Data System (ADS)

    McCarthy, C.; Savage, H. M.; Nettles, M.

    2015-12-01

    An understanding of the controls on ice stream flow is critical for improved predictions of sea level rise and glacier response to climate change. Basal sliding is one aspect of ice stream motion that has received relatively little attention. Although it is difficult and costly to measure direct motion at the base of a glacier, laboratory experiments can be used to recreate the physics of ice sliding over bedrock. Using a new, custom-built, servo-controlled biaxial loading apparatus, we are measuring the friction of polycrystalline ice samples sliding on rock in a double direct shear configuration. Temperature is maintained with an insulated cryostat that uses liquid cooling blocks and a programmable circulating bath. We will share results from a series of velocity stepping and slide-hold-slide experiments designed to measure key properties of rate- and state-dependent frictional behavior. The experimental conditions for the study are as follows: temperatures ranging from -20ºC to the pressure melting point; normal stresses of 20 - 200 kPa, velocities from 10-6 to 10-3 m s-1; and ambient pressure. Ice sample microstructure (grain size, porosity, purity) and surface roughness are carefully controlled and characterized before and after experiments to identify microstructural sources for macroscopic behavior. Careful monitoring of temperature at the sliding interface will elucidate the role of frictional heating/melting on both sliding behavior and microstructure evolution. By measuring rate-state friction parameters, we will explore the transition between stable sliding and stick-slip motion of glaciers and ice streams. These results can be directly compared to the differing sliding styles observed for ice streams feeding into the Ross Ice Shelf to infer characteristics of the bed interface and the bulk glacier. The values obtained from this study will provide better constraints for next generation modeling of glacier and ice-stream response to external forcing.

  13. Climax Granite, Nevada Test Site, as a host for a rock mechanics test facility related to the geologic disposal of high level nuclear wastes

    SciTech Connect

    Heuze, F.E.

    1981-02-01

    This document discusses the potential of the Climax pluton, at the Nevada Test Site, as the host for a granite mechanics test facility related to the geologic disposal of high-level nuclear waste. The Climax granitic pluton has been the site of three nuclear weapons effects tests: Hard Hat, Tiny Tot, and Piledriver. Geologic exploration and mapping of the granite body were performed at the occasion of these tests. Currently, it is the site Spent Fuel Test (SFT-C) conducted in the vicinity of and at the same depth as that of the Piledriver drifts. Significant exploration, mapping, and rock mechanics work have been performed and continue at this Piledriver level - the 1400 (ft) level - in the context of SFT-C. Based on our technical discussions, and on the review of the significant geological and rock mechanics work already achieved in the Climax pluton, based also on the ongoing work and the existing access and support, it is concluded that the Climax site offers great opportunities for a rock mechanics test facility. It is not claimed, however, that Climax is the only possible site or the best possible site, since no case has been made for another granite test facility in the United States. 12 figures, 3 tables.

  14. Thermal, Mechanical and Thermo-Mechanical Assessment of the Rock Mass Surrounding SKB's Prototype Repository at Äspö HRL

    NASA Astrophysics Data System (ADS)

    Lönnqvist, Margareta; Hökmark, Harald

    2016-04-01

    The Prototype Repository (PR) was a field test of six, electrically heated, full-scale waste containers resembling the key component of a KBS-3 nuclear waste repository. The design and heat load was similar to the proposed repository at Forsmark, Sweden. In this paper, the thermal, mechanical and thermo-mechanical response of the PR host rock to excavation and to the subsequent heating is assessed. The assessment is carried out using three-dimensional models (numerical and analytical) in combination with monitoring data and visual observations from the excavations. Certain measurements and observations agree well with results from the models. These include temperature measurements during the heating phase. Additional measurements include patterns of low-magnitude acoustic emission events around the deposition holes tracked during the excavation. The spatial distribution of these events coincide with regions of modelled high compressive stresses. Models with a simple fracture network, consisting of planar disks with laboratory-scale properties, appear to give upper bound estimates of the stress disturbances caused by a real fracture network. The magnitude of the modelled stresses around the deposition hole is typically below the spalling strength. The lack of any significant or systematic occurrence of spalling in the deposition hole walls supports the modelling results. Several instruments installed at different positions to monitor stress change, strain and deformation malfunctioned during the nearly 8-year-long monitoring period. Despite this, there is ample evidence to support the overall conclusion that the modelling results and observations are in sufficient agreement to strengthen the confidence in the modelling approach.

  15. The transition from hydrostatic to greater than hydrostatic fluid pressure in presently active continental hydrothermal systems in crystalline rock

    SciTech Connect

    Fournier, R.O. )

    1991-05-01

    Fluid flow at hydrostatic pressure (P{sub h}) is relatively common through fractures in silicic and in mafic crystalline rocks where temperatures are less than about 350-370C. In contrast, pore-fluid pressure (P{sub f}) > P{sub h} has been encountered at the bottom of 3 geothermal exploration wells that attained temperatures >370C (at Larderello, Italy, at Nesjavellir, Iceland, and at The Geysers, California). Chemical sealing by deposition of minerals in veins appears to have allowed the development of the high P{sub f} encountered in the above wells. The upper limit for the magnitude of P{sub f} that can be attained is controlled by either the onset of shear fracturing (where differential stress is relatively high) that reopens clogged veins, or the hydraulic opening of new or old fractures (at relatively low values of differential stress). The brittle-plastic transition for silicic rocks can occur at temperatures as high as 370-400C in tectonically active regions. In regions where high-temperature geothermal systems develop and persist, it appears that either strain rates commonly are in the range 10{sup {minus}12} to 10{sup {minus}13}, or that silicic rocks in the shallow crust generally behave rheologically more like wet quartz diorite than wet Westerly granite.

  16. Rheological and geodynamic controls on the mechanisms of subduction, HP/UHP exhumation and PT conditions within crustal rocks during continental collision: insights from numerical models

    NASA Astrophysics Data System (ADS)

    Burov, Evgueni

    2014-05-01

    Mechanisms of continental convergence are so versatile that it is impossible to elucidate them from conventional set of observations. Additional discriminatory data are needed such as those derived from petrology data, since burial/exhumation dynamics inferred from metamorphic P-T-t paths potentially provides independent constraints on the collision mechanism. While subduction of crustal rocks is increasingly accepted as common phenomenon inherent to convergent processes involving continental plates and micro-continents, the conditions of their formation and mechanisms of their exhumation in the form of high- and ultra-high-pressure (HP/UHP) units remain a subject of controversy. In particular, deep burial and exhumation of continental crust occur in various settings, including subduction of micro-continental terrains carried down with the subducting oceanic lithosphere and transition between the oceanic and continental subduction. Geodynamic inferences from P-T data can be made only after providing a consistent approach to decryption of both pressure and temperature in terms of depth or at least in terms of characteristic geodynamic conditions. Thermo-mechanical thermodynamically coupled numerical models of continental collision provide some elements of solution to this problem through testing various geodynamic scenarios within relatively unconstrained framework which allows for account of non-lithostatic pressure variations and for deviations of temperature from commonly inferred thermal models. We here explore several possible scenarios of subduction and exhumation of continental crust, and their relation to PT conditions and mechanisms of HP/UHP exhumation inferred from conceptual and thermo-mechanical numerical models accounting for thermo-rheological complexity and diversity of the continental lithosphere. Numerical experiments suggest that in most cases both exhumation and continental subduction are transient processes, so that long-lasting (> 10-15 Myr

  17. Workshop on hydrology of crystalline basement rocks

    SciTech Connect

    Davis, S.N.

    1981-08-01

    This workshop covered the following subjects: measurements in relatively shallow boreholes; measurement and interpretation of data from deep boreholes; hydrologic properties of crystalline rocks as interpreted by geophysics and field geology; rock mechanics related to hydrology of crystalline rocks; the possible contributions of modeling to the understanding of the hydrology of crystalline rocks; and geochemical interpretations of the hydrology of crystalline rocks. (MHR)

  18. The mechanism of copper activation of sphalerite

    NASA Astrophysics Data System (ADS)

    Gerson, Andrea R.; Lange, Angela G.; Prince, Kathryn E.; Smart, Roger St. C.

    1999-01-01

    On the basis of recent SIMS and XAFS measurements in conjunction with already published XPS results, a mechanism for the adsorption/absorption of Cu onto sphalerite is proposed. Under conditions of high pH and high nominal surface coverage of the sphalerite by the Cu, Cu(OH) 2 colloidal particles are observed on the sphalerite surfaces using SIMS. Under other conditions, SIMS measurements have indicated that adsorption of the Cu is essentially uniform over the sphalerite surface and is not related to low coordination sites on the surface of the sphalerite. Depth profiling of sphalerite surfaces with Cu adsorbed under conditions that do not result in Cu(OH) 2 colloidal particles show that the Cu adsorbed/absorbed on the sphalerite surface is largely in the first few atomic layers. XAFS analysis of Cu activated sphalerite has indicated that the Cu occupies a distorted trigonal planar geometry, coordinated to three S atoms, in both surface and bulk sites. In addition Cu(1s), absorption edges in XAFS show that both bulk and surface adsorbed copper have an oxidation state less than +1 with the surface Cu being slightly more oxidised than the bulk absorbed Cu. On the basis of the combined XPS, SIMS, XAFS and solution studies, a model is proposed that, on surface adsorption of Cu, the surface Zn(II) atoms are replaced by Cu(II) atoms which are then reduced in situ to Cu(I). This reduction is accompanied by the oxidation of the three neighbouring S atoms to an oxidation state of approximately -1.5. On bulk absorption of Cu atoms into the sphalerite lattice a distorted trigonal planar configuration is achieved through the breakage of a formerly tetrahedral Zn-S bond. The breakage of this bond results in a 3-fold coordinated Cu plus one S 3-fold coordinated to Zn atoms. The breakage of this bond leads to a greater reduction of the Cu than on surface absorption and also oxidation of the 3-fold coordinated S atom to an approximately -0.5 oxidation state. This model does not

  19. The Rock Physics Handbook

    NASA Astrophysics Data System (ADS)

    Mavko, Gary; Mukerji, Tapan; Dvorkin, Jack

    2003-10-01

    The Rock Physics Handbook conveniently brings together the theoretical and empirical relations that form the foundations of rock physics, with particular emphasis on seismic properties. It also includes commonly used models and relations for electrical and dielectric rock properties. Seventy-six articles concisely summarize a wide range of topics, including wave propagation, AVO-AVOZ, effective media, poroelasticity, pore fluid flow and diffusion. The book contains overviews of dispersion mechanisms, fluid substitution, and Vp-Vs relations. Useful empirical results on reservoir rocks and sediments, granular media, tables of mineral data, and an atlas of reservoir rock properties complete the text. This distillation of an otherwise scattered and eclectic mass of knowledge is presented in a form that can be immediately applied to solve real problems. Geophysics professionals, researchers and students as well as petroleum engineers, well log analysts, and environmental geoscientists will value The Rock Physics Handbook as a unique resource.

  20. The RhoE/ROCK/ARHGAP25 signaling pathway controls cell invasion by inhibition of Rac activity.

    PubMed

    Thuault, Sylvie; Comunale, Franck; Hasna, Jessy; Fortier, Mathieu; Planchon, Damien; Elarouci, Nabila; De Reynies, Aurélien; Bodin, Stéphane; Blangy, Anne; Gauthier-Rouvière, Cécile

    2016-09-01

    Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma of skeletal muscle origin in children and adolescents. Among RMS subtypes, alveolar rhabdomyosarcoma (ARMS), which is characterized by the presence of the PAX3-FOXO1A or PAX7-FOXO1A chimeric oncogenic transcription factor, is associated with poor prognosis and a strong risk of metastasis compared with the embryonal subtype (ERMS). To identify molecular pathways involved in ARMS aggressiveness, we first characterized the migratory behavior of cell lines derived from ARMS and ERMS biopsies using a three-dimensional spheroid cell invasion assay. ARMS cells were more invasive than ERMS cells and adopted an ellipsoidal morphology to efficiently invade the extracellular matrix. Moreover, the invasive potential of ARMS cells depended on ROCK activity, which is regulated by the GTPase RhoE. Specifically, RhoE expression was low in ARMS biopsies, and its overexpression in ARMS cells reduced their invasion potential. Conversely, ARHGAP25, a GTPase-activating protein for Rac, was up-regulated in ARMS biopsies. Moreover, we found that ARHGAP25 inhibits Rac activity downstream of ROCKII and is required for ARMS cell invasion. Our results indicate that the RhoE/ROCK/ARHGAP25 signaling pathway promotes ARMS invasive potential and identify these proteins as potential therapeutic targets for ARMS treatment. PMID:27413008

  1. Dynamic Assessment of Fibroblast Mechanical Activity during Rac-induced Cell Spreading in 3-D Culture

    PubMed Central

    Petroll, W. Matthew; Ma, Lisha; Kim, Areum; Ly, Linda; Vishwanath, Mridula

    2009-01-01

    The goal of this study was to determine the morphological and sub-cellular mechanical effects of Rac activation on fibroblasts within 3-D collagen matrices. Corneal fibroblasts were plated at low density inside 100 μm thick fibrillar collagen matrices and cultured for 1 to 2 days in serum-free media. Time-lapse imaging was then performed using Nomarski DIC. After an acclimation period, perfusion was switched to media containing PDGF. In some experiments, Y-27632 or blebbistatin were used to inhibit Rho-kinase (ROCK) or myosin II, respectively. PDGF activated Rac and induced cell spreading, which resulted in an increase in cell length, cell area, and the number of pseudopodial processes. Tractional forces were generated by extending pseudopodia, as indicated by centripetal displacement and realignment of collagen fibrils. Interestingly, the pattern of pseudopodial extension and local collagen fibril realignment was highly dependent upon the initial orientation of fibrils at the leading edge. Following ROCK or myosin II inhibition, significant ECM relaxation was observed, but small displacements of collagen fibrils continued to be detected at the tips of pseudopodia. Taken together, the data suggests that during Rac-induced cell spreading within 3-D matrices, there is a shift in the distribution of forces from the center to the periphery of corneal fibroblasts. ROCK mediates the generation of large myosin II-based tractional forces during cell spreading within 3-D collagen matrices, however residual forces can be generated at the tips of extending pseudopodia that are both ROCK and myosin II-independent. PMID:18452153

  2. Active recovery of the finger flexors enhances intermittent handgrip performance in rock climbers.

    PubMed

    Baláš, Jiří; Michailov, Michail; Giles, David; Kodejška, Jan; Panáčková, Michaela; Fryer, Simon

    2016-10-01

    This study aimed to (1) evaluate the effect of hand shaking during recovery phases of intermittent testing on the time-force characteristics of performance and muscle oxygenation, and (2) assess inter-individual variability in the time to achieve the target force during intermittent testing in rock climbers. Twenty-two participants undertook three finger flexor endurance tests at 60% of their maximal voluntary contraction until failure. Performances of a sustained contraction and two intermittent contractions, each with different recovery strategies, were analysed by time-force parameters and near-infrared spectroscopy. Recovery with shaking of the forearm beside the body led to a significantly greater intermittent test time (↑ 22%, P < .05), force-time integral (↑ 28%, P < .05) and faster muscle re-oxygenation (↑ 32%, P < .05), when compared to the hand over hold condition. Further, the ratio of intermittent to continuous test time distinguished specific aerobic muscular adaptations among sport climbers (2.02), boulderers (1.74) and lower grade climbers (1.25). Lower grade climbers and boulderers produced shorter duration contractions due to the slower development of target force during the intermittent test, indicating worse kinaesthetic differentiation. Both the type of recovery and climbing discipline determined muscle re-oxygenation and intermittent performance in rock climbers. PMID:27491378

  3. Geochemical and geochronological constraints on the origin and evolution of rocks in the active Woodlark Rift of Papua New Guinea

    NASA Astrophysics Data System (ADS)

    Zirakparvar, Nasser Alexander

    Tectonically active regions provide important natural laboratories to glean information that is applicable to developing a better understanding of the geologic record. One such area of the World is Papua New Guinea, much of which is situated in an active and transient plate boundary zone. The focus of this PhD research is to develop a better understanding of rocks in the active Woodlark Rift, situated in Papua New Guinea's southernmost reaches. In this region, rifting and lithospheric rupture is occurring within a former subduction complex where there is a history of continental subduction and (U)HP metamorphism. The lithostratigraphic units exposed in the Woodlark Rift provide an opportunity to better understand the records of plate boundary processes at many scales from micron-sized domains within individual minerals to regional geological relationships. This thesis is composed of three chapters that are independent of one another but are all related to the overall goal of developing a better understanding of the record of plate boundary processes in the rocks currently exposed in the Woodlark Rift. The first chapter, published in its entirety in Earth and Planetary Science Letters (2011 v. 309, p. 56 - 66), is entitled 'Lu-Hf garnet geochronology applied to plate boundary zones: Insights from the (U)HP terrane exhumed within the Woodlark Rift'. This chapter focuses on the use of the Lu-Hf isotopic system to date garnets in the Woodlark Rift. Major findings of this study are that some of the rocks in the Woodlark Rift preserve a Lu-Hf garnet isotopic record of initial metamorphism and continental subduction occurring in the Late Mesozoic, whereas others only preserve a record of tectonic processes related to lithospheric rupture during the initiation of rifting in the Late Cenozoic. The second chapter is entitled 'Geochemical and geochronological constraints on the origin of rocks in the active Woodlark Rift of Papua New Guinea: Recognizing the dispersed

  4. Intracellular mechanisms of lymphoid cell activation.

    PubMed

    Fresa, K; Hameed, M; Cohen, S

    1989-01-01

    Activation of lymphocytes for proliferation is associated with the appearance of an intracellular factor (ADR) that can induce DNA synthesis in isolated quiescent nuclei. ADR plays a role in the sequence of intracellular events leading to activation for IL-2-mediated proliferation. Because of the nature of the defining assay, the locus of ADR action appears to be near the terminal end of the transduction pathway. Interestingly, although lymphocytes from aged individuals respond poorly to proliferative stimuli, they appear to produce normal to above-normal levels of ADR. In contrast, their nuclei are only poorly responsive to stimulation by ADR. Preparations rich in ADR activity have proteolytic activity as well. In addition, aprotinin, as well as a variety of other protease inhibitors, suppresses ADR-induced DNA synthesis in a dose-dependent manner. ADR activity can be removed from active extracts by absorption with aprotinin-conjugated agarose beads, and can be removed from the beads by elution at pH 5.0. This latter suggests that ADR itself is a protease. However, its endogenous substrate is not yet known. We have also detected an inhibitor of ADR activity in the cytoplasm of resting lymphocytes. This is a heat-stable protein of approximately 60,000 Da. In addition to suppressing the interaction of ADR with quiescent nuclei, the inhibitor can suppress DNA synthetic activity of replicative nuclei isolated from mitogen-activated lymphocytes. Interestingly, these preparations had little or no activity on replicative nuclei derived from several neoplastic cell lines. The resistance of tumor cell nuclei to spontaneously occurring cytoplasmic inhibitory factors such as the one described here may provide one explanation for the loss of growth control in neoplastic cells. PMID:2642767

  5. Insights into Mechanism of Glucokinase Activation

    PubMed Central

    Liu, Shenping; Ammirati, Mark J.; Song, Xi; Knafels, John D.; Zhang, Jeff; Greasley, Samantha E.; Pfefferkorn, Jeffrey A.; Qiu, Xiayang

    2012-01-01

    Human glucokinase (GK) is a principal regulating sensor of plasma glucose levels. Mutations that inactivate GK are linked to diabetes, and mutations that activate it are associated with hypoglycemia. Unique kinetic properties equip GK for its regulatory role: although it has weak basal affinity for glucose, positive cooperativity in its binding of glucose causes a rapid increase in catalytic activity when plasma glucose concentrations rise above euglycemic levels. In clinical trials, small molecule GK activators (GKAs) have been efficacious in lowering plasma glucose and enhancing glucose-stimulated insulin secretion, but they carry a risk of overly activating GK and causing hypoglycemia. The theoretical models proposed to date attribute the positive cooperativity of GK to the existence of distinct protein conformations that interconvert slowly and exhibit different affinities for glucose. Here we report the respective crystal structures of the catalytic complex of GK and of a GK-glucose complex in a wide open conformation. To assess conformations of GK in solution, we also carried out small angle x-ray scattering experiments. The results showed that glucose dose-dependently converts GK from an apo conformation to an active open conformation. Compared with wild type GK, activating mutants required notably lower concentrations of glucose to be converted to the active open conformation. GKAs decreased the level of glucose required for GK activation, and different compounds demonstrated distinct activation profiles. These results lead us to propose a modified mnemonic model to explain cooperativity in GK. Our findings may offer new approaches for designing GKAs with reduced hypoglycemic risk. PMID:22298776

  6. Universal allosteric mechanism for Gα activation by GPCRs

    PubMed Central

    Flock, Tilman; Venkatakrishnan, A. J.; Kayikci, Melis; Tate, Christopher G.; Veprintsev, Dmitry B.; Babu, M. Madan

    2016-01-01

    G protein-coupled receptors (GPCRs) allosterically activate heterotrimeric G proteins and trigger GDP release. Given that there are ~800 human GPCRs and 16 different Gα proteins, does a universal allosteric mechanism govern Gα activation? Here we show that different GPCRs interact and activate Gα proteins through a highly conserved mechanism. Comparison of Gα with the small G protein Ras reveals how the evolution of short segments that can undergo disorder-order transitions decouple regions important for allosteric activation from receptor binding specificity. This might explain how the GPCR-Gα system diversified rapidly, whilst conserving the allosteric activation mechanism. PMID:26147082

  7. Universal allosteric mechanism for Gα activation by GPCRs.

    PubMed

    Flock, Tilman; Ravarani, Charles N J; Sun, Dawei; Venkatakrishnan, A J; Kayikci, Melis; Tate, Christopher G; Veprintsev, Dmitry B; Babu, M Madan

    2015-08-13

    G protein-coupled receptors (GPCRs) allosterically activate heterotrimeric G proteins and trigger GDP release. Given that there are ∼800 human GPCRs and 16 different Gα genes, this raises the question of whether a universal allosteric mechanism governs Gα activation. Here we show that different GPCRs interact with and activate Gα proteins through a highly conserved mechanism. Comparison of Gα with the small G protein Ras reveals how the evolution of short segments that undergo disorder-to-order transitions can decouple regions important for allosteric activation from receptor binding specificity. This might explain how the GPCR-Gα system diversified rapidly, while conserving the allosteric activation mechanism. PMID:26147082

  8. Rock Cycle Roulette.

    ERIC Educational Resources Information Center

    Schmidt, Stan M.; Palmer, Courtney

    2000-01-01

    Introduces an activity on the rock cycle. Sets 11 stages representing the transitions of an earth material in the rock cycle. Builds six-sided die for each station, and students move to the stations depending on the rolling side of the die. Evaluates students by discussing several questions in the classroom. Provides instructional information for…

  9. Collecting Rocks.

    ERIC Educational Resources Information Center

    Barker, Rachel M.

    One of a series of general interest publications on science topics, the booklet provides those interested in rock collecting with a nontechnical introduction to the subject. Following a section examining the nature and formation of igneous, sedimentary, and metamorphic rocks, the booklet gives suggestions for starting a rock collection and using…

  10. Rock Art

    ERIC Educational Resources Information Center

    Henn, Cynthia A.

    2004-01-01

    There are many interpretations for the symbols that are seen in rock art, but no decoding key has ever been discovered. This article describes one classroom's experiences with a lesson on rock art--making their rock art and developing their own personal symbols. This lesson allowed for creativity, while giving an opportunity for integration…

  11. Activation Mechanisms in Ion-Implanted Gallium -

    NASA Astrophysics Data System (ADS)

    Morris, Neil

    Available from UMI in association with The British Library. Rapid Thermal Annealing has been used to study the electrical activation of a range of donor and acceptor species in ion-implanted GaAs. By varying the time and temperature of the post implant anneal, it was found that the activation processes for most implants can be characterised in terms of two distinct regions. The first of these occurs at short annealing times, where the electrical activity is seen to follow a time-dependent behaviour. At longer annealing times, however, a time-independent saturation value is reached, this value being dependent on the annealing temperature. By analysing the data from Be, Mg, S and Se implants in GaAs, a comprehensive model has been evolved for the time and temperature dependence of the sheet electrical properties. Application of this model to each of the ions studied suggests that the activation processes may be dominated by the extent to which ions form impurity-vacancy complexes. An analysis of the time-dependent regime also shows that, at short annealing times, the mobile species is more likely to be the substrate atoms (or vacancies) rather than the implanted impurities. In the time-dependent region, the values of diffusion energy were found to be between 2.3 to 3.0 eV for all ions, these values corresponding to a diffusion of Ga or As vacancies (or atoms). In the saturation region, activation energies of 0.3 to 0.4 eV and 1.0 to 1.2 eV were obtained for the activation processes of interstitial or complexed impurities respectively.

  12. Geochemistry of Phosphorus and Nitrogen in Volcanic Rocks Altered by Submarine Hydrothermal Activities at the Suiyo Seamount in Japan

    NASA Astrophysics Data System (ADS)

    Noda, M.; Kakegawa, T.; Naraoka, H.; Marumo, K.; Urabe, T.

    2002-12-01

    Phosphorus and nitrogen are essential major elements for all microorganisms. In order to understand the ecological conditions of subvent microorganisms and thermophilic microorganisms on ocean floor, it is necessary to understand the behavior of bio-essential elements not only in hydrothermal fluids but also in the subvent environment. Nine sites of hydrothermal discharging area were drilled in the Suiyo volcanic caldera, Izu-Ogasawara (Bonin) island-arc, western Pacific. Approximately 2 to 10 m deep drill core samples were recovered in the last two years. Chemical compositions and hydrothermal mineral assemblages in the drilled core samples were determined by XRF, ICP-MS, and XRD. Morphology of phosphorous-bearing minerals and their chemistry were examined by electron microprobe. Nitrogen isotopes were measured by the EA-IRMS system. Primary igneous-rock texture (such as euhedral plagioclase phenocryst) is found in the less altered rocks. They often associated with montmorillonite. Highly altered rocks are divided into two groups. First group is characterized by extensive (up to 90%) replacement of primary igneous mineral assemblage by chlorite, mica and sulfide. Second group is cemented with large amounts of sulfates with sulfide (mainly pyrite). It is found in a few drill core sections that hydrothermal hydrous silicate minerals change with depth from montmorillonite to chlorite and mica through mixed layer of chlorite/montmorillonite. This may suggest the more extensive and higher temperature alteration in deeper zones in a certain area. Electron microprobe analyses and bulk chemical composition indicate that the depletion of phosphorous in altered rocks (below 0.1 wt%) but enrichment of phosphorous in sulfide zones. This suggests that phosphorous was easily dissolved from igneous rocks by hydrothermal process, but readily precipitated with sulfides. The reason for co-precipitation of phosphates with sulfides is not certain, but such co-precipitation mechanism

  13. Joint Commission on rock properties

    NASA Astrophysics Data System (ADS)

    A joint commission on Rock Properties for Petroleum Engineers (RPPE) has been established by the International Society of Rock Mechanics and the Society of Petroleum Engineers to set up data banks on the properties of sedimentary rocks encountered during drilling. Computer-based data banks of complete rock properties will be organized for sandstones (GRESA), shales (ARSHA) and carbonates (CARCA). The commission hopes to access data sources from members of the commission, private companies and the public domain.

  14. AMPK activators: mechanisms of action and physiological activities.

    PubMed

    Kim, Joungmok; Yang, Goowon; Kim, Yeji; Kim, Jin; Ha, Joohun

    2016-01-01

    AMP-activated protein kinase (AMPK) is a central regulator of energy homeostasis, which coordinates metabolic pathways and thus balances nutrient supply with energy demand. Because of the favorable physiological outcomes of AMPK activation on metabolism, AMPK has been considered to be an important therapeutic target for controlling human diseases including metabolic syndrome and cancer. Thus, activators of AMPK may have potential as novel therapeutics for these diseases. In this review, we provide a comprehensive summary of both indirect and direct AMPK activators and their modes of action in relation to the structure of AMPK. We discuss the functional differences among isoform-specific AMPK complexes and their significance regarding the development of novel AMPK activators and the potential for combining different AMPK activators in the treatment of human disease. PMID:27034026

  15. AMPK activators: mechanisms of action and physiological activities

    PubMed Central

    Kim, Joungmok; Yang, Goowon; Kim, Yeji; Kim, Jin; Ha, Joohun

    2016-01-01

    AMP-activated protein kinase (AMPK) is a central regulator of energy homeostasis, which coordinates metabolic pathways and thus balances nutrient supply with energy demand. Because of the favorable physiological outcomes of AMPK activation on metabolism, AMPK has been considered to be an important therapeutic target for controlling human diseases including metabolic syndrome and cancer. Thus, activators of AMPK may have potential as novel therapeutics for these diseases. In this review, we provide a comprehensive summary of both indirect and direct AMPK activators and their modes of action in relation to the structure of AMPK. We discuss the functional differences among isoform-specific AMPK complexes and their significance regarding the development of novel AMPK activators and the potential for combining different AMPK activators in the treatment of human disease. PMID:27034026

  16. Rho-associated protein kinase 1 (ROCK1) is increased in Alzheimer's disease and ROCK1 depletion reduces amyloid-β levels in brain.

    PubMed

    Henderson, Benjamin W; Gentry, Erik G; Rush, Travis; Troncoso, Juan C; Thambisetty, Madhav; Montine, Thomas J; Herskowitz, Jeremy H

    2016-08-01

    Alzheimer's disease (AD) is the leading cause of dementia and mitigating amyloid-β (Aβ) levels may serve as a rational therapeutic avenue to slow AD progression. Pharmacologic inhibition of the Rho-associated protein kinases (ROCK1 and ROCK2) is proposed to curb Aβ levels, and mechanisms that underlie ROCK2's effects on Aβ production are defined. How ROCK1 affects Aβ generation remains a critical barrier. Here, we report that ROCK1 protein levels were elevated in mild cognitive impairment due to AD (MCI) and AD brains compared to controls. Aβ42 oligomers marginally increased ROCK1 and ROCK2 protein levels in neurons but strongly induced phosphorylation of Lim kinase 1 (LIMK1), suggesting that Aβ42 activates ROCKs. RNAi depletion of ROCK1 or ROCK2 suppressed endogenous Aβ40 production in neurons, and Aβ40 levels were reduced in brains of ROCK1 heterozygous knock-out mice compared to wild-type littermate controls. ROCK1 knockdown decreased amyloid precursor protein (APP), and treatment with bafilomycin accumulated APP levels in neurons depleted of ROCK1. These observations suggest that reduction of ROCK1 diminishes Aβ levels by enhancing APP protein degradation. Collectively, these findings support the hypothesis that both ROCK1 and ROCK2 are therapeutic targets to combat Aβ production in AD. Mitigating amyloid-β (Aβ) levels is a rational strategy for Alzheimer's disease (AD) treatment, however, therapeutic targets with clinically available drugs are lacking. We hypothesize that Aβ accumulation in mild cognitive impairment because of AD (MCI) and AD activates the RhoA/ROCK pathway which in turn fuels production of Aβ. Escalation of this cycle over the course of many years may contribute to the buildup of amyloid pathology in MCI and/or AD. PMID:27246255

  17. Rho-associated protein kinase 1 (ROCK1) is increased in Alzheimer's disease and ROCK1 depletion reduces amyloid-β levels in brain.

    PubMed

    Henderson, Benjamin W; Gentry, Erik G; Rush, Travis; Troncoso, Juan C; Thambisetty, Madhav; Montine, Thomas J; Herskowitz, Jeremy H

    2016-08-01

    Alzheimer's disease (AD) is the leading cause of dementia and mitigating amyloid-β (Aβ) levels may serve as a rational therapeutic avenue to slow AD progression. Pharmacologic inhibition of the Rho-associated protein kinases (ROCK1 and ROCK2) is proposed to curb Aβ levels, and mechanisms that underlie ROCK2's effects on Aβ production are defined. How ROCK1 affects Aβ generation remains a critical barrier. Here, we report that ROCK1 protein levels were elevated in mild cognitive impairment due to AD (MCI) and AD brains compared to controls. Aβ42 oligomers marginally increased ROCK1 and ROCK2 protein levels in neurons but strongly induced phosphorylation of Lim kinase 1 (LIMK1), suggesting that Aβ42 activates ROCKs. RNAi depletion of ROCK1 or ROCK2 suppressed endogenous Aβ40 production in neurons, and Aβ40 levels were reduced in brains of ROCK1 heterozygous knock-out mice compared to wild-type littermate controls. ROCK1 knockdown decreased amyloid precursor protein (APP), and treatment with bafilomycin accumulated APP levels in neurons depleted of ROCK1. These observations suggest that reduction of ROCK1 diminishes Aβ levels by enhancing APP protein degradation. Collectively, these findings support the hypothesis that both ROCK1 and ROCK2 are therapeutic targets to combat Aβ production in AD. Mitigating amyloid-β (Aβ) levels is a rational strategy for Alzheimer's disease (AD) treatment, however, therapeutic targets with clinically available drugs are lacking. We hypothesize that Aβ accumulation in mild cognitive impairment because of AD (MCI) and AD activates the RhoA/ROCK pathway which in turn fuels production of Aβ. Escalation of this cycle over the course of many years may contribute to the buildup of amyloid pathology in MCI and/or AD.

  18. [Molecular mechanism at the presynaptic active zone].

    PubMed

    Ohtsuka, Toshihisa

    2011-07-01

    Our higher brain functions such as learning and memory, emotion, and consciousness depend on the precise regulation of complicated neural networks in the brain. Neurons communicate with each other through the synapse, which comprise 3 regions: the presynapse, synaptic cleft, and postsynapse. The active zone (AZ) beneath the presynaptic membrane is the principal site for Ca2+ -dependent neurotransmitter release: AZ is involved in determining the site for docking and synaptic vesicle fusion. Presently, the full molecular composition of AZ is unclear, but it is known to contain several AZ-specific proteins, including cytomatrix of the active zone-associated protein (CAST)/ERC2, ELKS, RIM1, Munc13-1, Piccolo/Aczonin, and Bassoon. CAST and ELKS are novel active zone proteins that directly bind to Rab3-interacting molecules (RIMs), Bassoon, and Piccolo, and are thought to play a role in neurotransmitter release by binding these to AZ proteins. In this review, current advances in studies on AZ structure and function have been summarized, and the focus is mainly on protein-protein interactions among the AZ proteins.

  19. The effect of ROCK-1 activity change on the adhesive and invasive ability of Y79 retinoblastoma cells

    PubMed Central

    2014-01-01

    Background Retinoblastoma (Rb) is the most common intraocular tumor in childhood worldwide. It is a deadly pediatric eye cancer. The main cause of death in Rb patients is intracranial and systemic metastasis. ROCK is the main downstream effector of Ras-homologous (Rho) family of GTPases which are involved in many cellular functions, such as cell proliferation, invasion and metastasis. Overexpression of ROCK promotes invasion and metastasis of many solid tumors. However, the effect of ROCK in Rb is largely unknown. Methods ROCK-1 and ROCK-2 mRNA expression in Y79 cell lines were examined by RT-PCR. Protein expression in the Y79 cell line were examined by western blot analyses. ROCK-1 and ROCK-2 siRNA were transfected into Y79 cells with Lipofectamine 2000. Cell proliferation was evaluated by CCK-8 assay after exposure to ROCK inhibitor (Y-27632). We examined the effect of ROCK inhibitors (Y-27632, ROCK-1 and ROCK-2 siRNA) on Y79 cell adhesive capacity by cell adhesion assay. Cell invasion assay through matrigel was used to study the effect of ROCK inhibitors on Y79 cell invasive capacity. Results The expression of mRNA of ROCK-1 was more than that of ROCK-2 in the Y79 cell line. The protein expression levels of ROCK-1 and ROCK-2 were downregulated in the cells transfected with siRNA. Y-27632 treatment didn’t lead to any changes of Y79 cells proliferation. Adhesive ability of Y79 cells was enhanced following Y-27632 or ROCK-1 siRNA treatment. The invasive capacity of Y79 cells showed an inverse relationship with increasing Y-27632 concentration. Invasiveness of Y79 cells also decreased in Y79 cells transfected with ROCK-1 siRNA. However, there was no change in adhesive ability or invasive capacity in Y79 cells transfected with siRNA against ROCK-2. Conclusions The findings of this study demonstrate that ROCK-1 protein plays a key role in regulating metastasis and invasion of Y79 cells, suggesting that the ROCK-1 dependent pathway may be a potential target for

  20. Developing a Virtual Rock Deformation Laboratory

    NASA Astrophysics Data System (ADS)

    Zhu, W.; Ougier-simonin, A.; Lisabeth, H. P.; Banker, J. S.

    2012-12-01

    Experimental rock physics plays an important role in advancing earthquake research. Despite its importance in geophysics, reservoir engineering, waste deposits and energy resources, most geology departments in U.S. universities don't have rock deformation facilities. A virtual deformation laboratory can serve as an efficient tool to help geology students naturally and internationally learn about rock deformation. Working with computer science engineers, we built a virtual deformation laboratory that aims at fostering user interaction to facilitate classroom and outreach teaching and learning. The virtual lab is built to center around a triaxial deformation apparatus in which laboratory measurements of mechanical and transport properties such as stress, axial and radial strains, acoustic emission activities, wave velocities, and permeability are demonstrated. A student user can create her avatar to enter the virtual lab. In the virtual lab, the avatar can browse and choose among various rock samples, determine the testing conditions (pressure, temperature, strain rate, loading paths), then operate the virtual deformation machine to observe how deformation changes physical properties of rocks. Actual experimental results on the mechanical, frictional, sonic, acoustic and transport properties of different rocks at different conditions are compiled. The data acquisition system in the virtual lab is linked to the complied experimental data. Structural and microstructural images of deformed rocks are up-loaded and linked to different deformation tests. The integration of the microstructural image and the deformation data allows the student to visualize how forces reshape the structure of the rock and change the physical properties. The virtual lab is built using the Game Engine. The geological background, outstanding questions related to the geological environment, and physical and mechanical concepts associated with the problem will be illustrated on the web portal. In

  1. Investigations of Near-Field Thermal-Hydrologic-Mechanical-Chemical Models for Radioactive Waste Disposal in Clay/Shale Rock

    SciTech Connect

    Liu, H.H.; Li, L.; Zheng, L.; Houseworth, J.E.; Rutqvist, J.

    2011-06-20

    Clay/shale has been considered as potential host rock for geological disposal of high-level radioactive waste throughout the world, because of its low permeability, low diffusion coefficient, high retention capacity for radionuclides, and capability to self-seal fractures. For example, Callovo-Oxfordian argillites at the Bure site, France (Fouche et al., 2004), Toarcian argillites at the Tournemire site, France (Patriarche et al., 2004), Opalinus Clay at the Mont Terri site, Switzerland (Meier et al., 2000), and Boom clay at the Mol site, Belgium (Barnichon and Volckaert, 2003) have all been under intensive scientific investigation (at both field and laboratory scales) for understanding a variety of rock properties and their relationships to flow and transport processes associated with geological disposal of radioactive waste. Figure 1-1 presents the distribution of clay/shale formations within the USA.

  2. Mechanism for Clastogenic Activity of Naphthalene

    SciTech Connect

    Buchholz, Bruce A.

    2015-09-29

    Naphthalene incubations form DNA adducts in vitro in a dose dependent manner in both mouse and rat tissues. Rodent tissue incubations with naphthalene indicate that naphthalene forms as many DNA adducts as Benzo(a)pyrene, a known DNA binding carcinogen. The mouse airway has the greatest number of DNA adducts, corresponding to the higher metabolic activation of naphthalene in this location. Both rat tissues, the rat olfactory (tumor target) and the airways (non-tumor target), have similar levels of NA-DNA adducts, indicating that short term measures of initial adduct formation do not directly correlate with sites of tumor formation in the NTP bioassays.

  3. Mechanism of antibacterial activity of copper nanoparticles

    NASA Astrophysics Data System (ADS)

    Chatterjee, Arijit Kumar; Chakraborty, Ruchira; Basu, Tarakdas

    2014-04-01

    In a previous communication, we reported a new method of synthesis of stable metallic copper nanoparticles (Cu-NPs), which had high potency for bacterial cell filamentation and cell killing. The present study deals with the mechanism of filament formation and antibacterial roles of Cu-NPs in E. coli cells. Our results demonstrate that NP-mediated dissipation of cell membrane potential was the probable reason for the formation of cell filaments. On the other hand, Cu-NPs were found to cause multiple toxic effects such as generation of reactive oxygen species, lipid peroxidation, protein oxidation and DNA degradation in E. coli cells. In vitro interaction between plasmid pUC19 DNA and Cu-NPs showed that the degradation of DNA was highly inhibited in the presence of the divalent metal ion chelator EDTA, which indicated a positive role of Cu2+ ions in the degradation process. Moreover, the fast destabilization, i.e. the reduction in size, of NPs in the presence of EDTA led us to propose that the nascent Cu ions liberated from the NP surface were responsible for higher reactivity of the Cu-NPs than the equivalent amount of its precursor CuCl2; the nascent ions were generated from the oxidation of metallic NPs when they were in the vicinity of agents, namely cells, biomolecules or medium components, to be reduced simultaneously.

  4. Mechanism of antibacterial activity of copper nanoparticles.

    PubMed

    Chatterjee, Arijit Kumar; Chakraborty, Ruchira; Basu, Tarakdas

    2014-04-01

    In a previous communication, we reported a new method of synthesis of stable metallic copper nanoparticles (Cu-NPs), which had high potency for bacterial cell filamentation and cell killing. The present study deals with the mechanism of filament formation and antibacterial roles of Cu-NPs in E. coli cells. Our results demonstrate that NP-mediated dissipation of cell membrane potential was the probable reason for the formation of cell filaments. On the other hand, Cu-NPs were found to cause multiple toxic effects such as generation of reactive oxygen species, lipid peroxidation, protein oxidation and DNA degradation in E. coli cells. In vitro interaction between plasmid pUC19 DNA and Cu-NPs showed that the degradation of DNA was highly inhibited in the presence of the divalent metal ion chelator EDTA, which indicated a positive role of Cu(2+) ions in the degradation process. Moreover, the fast destabilization, i.e. the reduction in size, of NPs in the presence of EDTA led us to propose that the nascent Cu ions liberated from the NP surface were responsible for higher reactivity of the Cu-NPs than the equivalent amount of its precursor CuCl2; the nascent ions were generated from the oxidation of metallic NPs when they were in the vicinity of agents, namely cells, biomolecules or medium components, to be reduced simultaneously. PMID:24584282

  5. Crustal Seismic Anisotropy Produced by Rock Fabric Terranes in the Taiwan Central Range Deformational Orogen: Integrative Study Combining Rock Physics, Structural Geology, and Passive/Active-Source Seismology

    NASA Astrophysics Data System (ADS)

    Okaya, D. A.; Ross, Z.; Christensen, N. I.; Wu, F. T.; Byrne, T. B.

    2014-12-01

    The island of Taiwan is currently under construction due to the collision of the northwestern corner of the Philippine Sea plate and the embedded Luzon island arc with the larger continental Eurasian plate. This collision is responsible for the current growth of the Central Range that dominates the eastern half of the island. An international collaboration involving several USA and Taiwan universities and academic institutions was formed to study how the orogen evolves through time and to understand the role of a colliding island arc in mountain building. The project, Taiwan Integrated Geodynamics Research (TAIGER), was funded by NSF-Continental Dynamics and Taiwan National Science Council. The Central Range grows at one of the most rapid rates of uplift in the world, exposing metamorphic rocks that were once at least 10 km deep. The range offers unique opportunities for studies of crustal seismic anisotropy for two major reasons: (1) its geological makeup is conducive for producing crustal seismic anisotropy; that is, the rocks are highly foliated; and (2) a seismological data volume of significant breadth offers extensive coverage of sources and recording stations throughout the region. We carried out a crustal shear wave splitting study by data mining 3300 local earthquakes collected in the TAIGER 2009 sea-land experiment. We used an automated P and S wave arrival time picking method (Ross and Ben-Zion, 2014) applied to over 100,000 event-station pairs. These data were analyzed for shear-wave splitting using the MFAST automated package (Savage et al., 2010), producing 3300 quality shear wave split measurements. The splitting results were then station-averaged. The results show NNE to NE orientation trends that are consistent with regional cleavage strikes. Average crustal shear wave split time is 0.244 sec. These measurements are consistent with rock physics measurements of Central Range slate and metamorphic acoustic velocities. The splits exhibit orientations

  6. Focus on the Rock.

    ERIC Educational Resources Information Center

    Shewell, John

    1994-01-01

    Describes historical accounts of the manipulation and importance of the Earth and its mineral resources. A foldout, "Out of the Rock," provides a collection of activities and information that helps make integration of the aforementioned concepts easy. (ZWH)

  7. Rock Bites into 'Bounce'

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This panoramic camera image from the Mars Exploration Rover Opportunity features the 6.44 millimeter (0.25 inch) deep hole ground into the rock dubbed 'Bounce' by the rover's rock abrasion tool. The tool took 2 hours and 15 minutes to grind the hole on sol 66 of the rover's journey. A combination of limited solar power and the rock's jagged texture led the rock abrasion tool team to set very aggressive grinding parameters to ensure that the end result was a full circle, suitable for a thorough read from the rover's spectrometers.

    Bounce's markedly different appearance (when compared to the rocks that were previously examined in the Eagle Crater outcrop) made it a natural target for rover research. In order to achieve an ideal position from which to grind into the rock, Opportunity moved in very close with its right wheel next to Bounce. In this image, the panoramic camera on the rover's mast is looking down, catching the tip of the solar panel which partially blocks the full circle ground by the rock abrasion tool.

    The outer ring consists of the cuttings from the rock, pushed out by the brushes on the grinding instrument. The dark impression at the top of the outer circle was caused by the instrument's contact mechanism which serves to stabilize it while grinding.

  8. Short and long term release mechanisms of arsenic, selenium and boron from a tunnel-excavated sedimentary rock under in situ conditions.

    PubMed

    Tamoto, Shuichi; Tabelin, Carlito Baltazar; Igarashi, Toshifumi; Ito, Mayumi; Hiroyoshi, Naoki

    2015-01-01

    Sedimentary rocks of marine origin excavated from tunnel construction projects usually contain background levels of hazardous trace elements, but when exposed to the environment, they generate leachates with concentrations of arsenic (As), selenium (Se) and boron (B) exceeding the WHO guideline for drinking water. In this study, the leaching of As, Se and B was evaluated under in situ conditions at various flow patterns, particle size distributions and column thicknesses. The results showed that these trace elements were leached out of the rock via short and long term mechanisms. In the short term, all three elements were rapidly and simultaneously released due to the dissolution of soluble evaporite salts formed from entrapped sea water of the Cretaceous. After their rapid release, however, these trace elements behaved differently as a result of their contrasting adsorption affinities onto minerals like clays and Fe-oxyhydroxides, which were further influenced by the pH, presence of coexisting ions and speciation of the trace elements. Selenium was quickly and easily transported out of the columns because it was mostly present as the very mobile selenate ion (Se[VI]). In comparison, the migration of As and B was hindered by adsorption reactions onto mineral phases of the rock. Boron was initially the least mobile among the three because of its preferential adsorption onto clay minerals that was further enhanced by the slightly alkaline pH and high concentrations of Ca(2+) and Na(+). However, it was gradually re-mobilized in the latter part of the experiments because it was only weakly adsorbed via outer sphere complexation reactions. In the long term, the rock continued to release substantial amounts of As, Se and B via pyrite oxidation and adsorption/desorption reactions, which were regulated by the temperature and rainfall intensity/frequency on site.

  9. Hydrogeologic controls imposed by mechanical stratigraphy in layered rocks of the Chateauguay River Basin, a U.S.-Canada transborder aquifer

    USGS Publications Warehouse

    Morin, Roger H.; Godin, Rejean; Nastev, Miroslav; Rouleau, Alain

    2007-01-01

    [1] The Châteauguay River Basin delineates a transborder watershed with roughly half of its surface area located in northern New York State and half in southern Québec Province, Canada. As part of a multidisciplinary study designed to characterize the hydrogeologic properties of this basin, geophysical logs were obtained in 12 wells strategically located to penetrate the four major sedimentary rock formations that constitute the regional aquifers. The layered rocks were classified according to their elastic properties into three primary units: soft sandstone, hard sandstone, and dolostone. Downhole measurements were analyzed to identify fracture patterns associated with each unit and to evaluate their role in controlling groundwater flow. Fracture networks are composed of orthogonal sets of laterally extensive, subhorizontal bedding plane partings and bed-delimited, subvertical joints with spacings that are consistent with rock mechanics principles and stress models. The vertical distribution of transmissive zones is confined to a few select bedding plane fractures, with soft sandstone having the fewest (one per 70-m depth) and hard sandstone the most (five per 70-m depth). Bed-normal permeability is examined using a probabilistic model that considers the lengths of flow paths winding along joints and bedding plane fractures. Soft sandstone has the smallest bed-normal permeability primarily because of its wide, geomechanically undersaturated joint spacing. Results indicate that the three formations have similar values of bulk transmissivity, within roughly an order of magnitude, but that each rock unit has its own unique system of groundwater flow paths that constitute that transmissivity.

  10. Short and long term release mechanisms of arsenic, selenium and boron from a tunnel-excavated sedimentary rock under in situ conditions

    NASA Astrophysics Data System (ADS)

    Tamoto, Shuichi; Tabelin, Carlito Baltazar; Igarashi, Toshifumi; Ito, Mayumi; Hiroyoshi, Naoki

    2015-04-01

    Sedimentary rocks of marine origin excavated from tunnel construction projects usually contain background levels of hazardous trace elements, but when exposed to the environment, they generate leachates with concentrations of arsenic (As), selenium (Se) and boron (B) exceeding the WHO guideline for drinking water. In this study, the leaching of As, Se and B was evaluated under in situ conditions at various flow patterns, particle size distributions and column thicknesses. The results showed that these trace elements were leached out of the rock via short and long term mechanisms. In the short term, all three elements were rapidly and simultaneously released due to the dissolution of soluble evaporite salts formed from entrapped sea water of the Cretaceous. After their rapid release, however, these trace elements behaved differently as a result of their contrasting adsorption affinities onto minerals like clays and Fe-oxyhydroxides, which were further influenced by the pH, presence of coexisting ions and speciation of the trace elements. Selenium was quickly and easily transported out of the columns because it was mostly present as the very mobile selenate ion (Se[VI]). In comparison, the migration of As and B was hindered by adsorption reactions onto mineral phases of the rock. Boron was initially the least mobile among the three because of its preferential adsorption onto clay minerals that was further enhanced by the slightly alkaline pH and high concentrations of Ca2 + and Na+. However, it was gradually re-mobilized in the latter part of the experiments because it was only weakly adsorbed via outer sphere complexation reactions. In the long term, the rock continued to release substantial amounts of As, Se and B via pyrite oxidation and adsorption/desorption reactions, which were regulated by the temperature and rainfall intensity/frequency on site.

  11. Short and long term release mechanisms of arsenic, selenium and boron from a tunnel-excavated sedimentary rock under in situ conditions.

    PubMed

    Tamoto, Shuichi; Tabelin, Carlito Baltazar; Igarashi, Toshifumi; Ito, Mayumi; Hiroyoshi, Naoki

    2015-01-01

    Sedimentary rocks of marine origin excavated from tunnel construction projects usually contain background levels of hazardous trace elements, but when exposed to the environment, they generate leachates with concentrations of arsenic (As), selenium (Se) and boron (B) exceeding the WHO guideline for drinking water. In this study, the leaching of As, Se and B was evaluated under in situ conditions at various flow patterns, particle size distributions and column thicknesses. The results showed that these trace elements were leached out of the rock via short and long term mechanisms. In the short term, all three elements were rapidly and simultaneously released due to the dissolution of soluble evaporite salts formed from entrapped sea water of the Cretaceous. After their rapid release, however, these trace elements behaved differently as a result of their contrasting adsorption affinities onto minerals like clays and Fe-oxyhydroxides, which were further influenced by the pH, presence of coexisting ions and speciation of the trace elements. Selenium was quickly and easily transported out of the columns because it was mostly present as the very mobile selenate ion (Se[VI]). In comparison, the migration of As and B was hindered by adsorption reactions onto mineral phases of the rock. Boron was initially the least mobile among the three because of its preferential adsorption onto clay minerals that was further enhanced by the slightly alkaline pH and high concentrations of Ca(2+) and Na(+). However, it was gradually re-mobilized in the latter part of the experiments because it was only weakly adsorbed via outer sphere complexation reactions. In the long term, the rock continued to release substantial amounts of As, Se and B via pyrite oxidation and adsorption/desorption reactions, which were regulated by the temperature and rainfall intensity/frequency on site. PMID:25747140

  12. RATDAMPER - A Numerical Model for Coupling Mechanical and Hydrological Properties within the Disturbed Rock Zone at the Waste Isolation Pilot Plant

    SciTech Connect

    RATH,JONATHAN S.; PFEIFLE,T.W.; HUNSCHE,U.

    2000-11-27

    A numerical model for predicting damage and permeability in the disturbed rock zone (DRZ) has been developed. The semi-empirical model predicts damage based on a function of stress tensor invariant. For a wide class of problems hydrologic/mechanical coupling is necessary for proper analysis. The RATDAMPER model incorporates dilatant volumetric strain and permeability. The RATDAMPER model has been implemented in a weakly coupled code, which combines a finite element structural code and a finite difference multi-phase fluid flow code. Using the development of inelastic volumetric strain, a value of permeability can be assigned. This flexibility allows empirical permeability functional relationships to be evaluated.

  13. Transient fluvial incision and spatial distribution of active rock uplift in the Uspallata-Calingasta-Iglesia Valley, Central Argentina.

    NASA Astrophysics Data System (ADS)

    Val, P.; Hoke, G. D.

    2014-12-01

    In the southern portion of the Pampean flat-slab subduction zone, a narrow, elongate intermontane depression separates the Precordillera fold-and-thrust belt from the Frontal and Principal Cordilleras of the high Andes. This depression, the Uspallata-Callingasta-Iglesia Valley, is divided into three catchments based on drainage divides between three large, transverse rivers. These catchments have their headwaters in the western Precordillera, Frontal Cordillera and Principal Cordillera ranges. Deformation is thought to have ceased near the core of the range (Frontal and Principal Cordilleras) and propagated to the east during the Miocene to present. In this study, river profiles and knickpoint topographic data are assessed to determine the distribution of active rock uplift and amount of fluvial incision in the Calingasta and Iglesia valleys. Slope-area data extracted from a digital elevation model consistently show slope-break knickpoints separating distinct sections of river profiles, which suggests relative bedrock uplift. Catchment-wide, normalized steepness indices (ksn) - using a concavity index of 0.45 (common in steady state profiles in the study area) - for both basins revealed consistently high ksn signals (>300) in the main stems and tributaries draining the Frontal Cordillera and the western Precordillera. The highest ksn values are situated in an elevation range of 2500 to 4000 m, west of the fold and thrust belt in the Principal and Frontal Cordilleras. Slope-break knickpoints are not clustered in elevation and there is no obvious correlation between upstream drainage area at knickpoints and respective distance from the tributaries' mouths, which suggests that they are not part of an upstream migrating wave of knickpoints. The data presented here indicate active rock uplift in the eastern edge of the Frontal Cordillera, which was previously considered inactive.

  14. Fault Rock Variation as a Function of Host Rock Lithology

    NASA Astrophysics Data System (ADS)

    Fagereng, A.; Diener, J.

    2013-12-01

    Fault rocks contain an integrated record of the slip history of a fault, and thereby reflect the deformation processes associated with fault slip. Within the Aus Granulite Terrane, Namibia, a number of Jurassic to Cretaceous age strike-slip faults cross-cut Precambrian high grade metamorphic rocks. These strike-slip faults were active at subgreenschist conditions and occur in a variety of host rock lithologies. Where the host rock contains significant amounts of hydrous minerals, representing granulites that have undergone retrogressive metamorphism, the fault rock is dominated by hydrothermal breccias. In anhydrous, foliated rocks interlayered with minor layers containing hydrous phyllosilicates, the fault rock is a cataclasite partially cemented by jasper and quartz. Where the host rock is an isotropic granitic rock the fault rock is predominantly a fine grained black fault rock. Cataclasites and breccias show evidence for multiple deformation events, whereas the fine grained black fault rocks appear to only record a single slip increment. The strike-slip faults observed all formed in the same general orientation and at a similar time, and it is unlikely that regional stress, strain rate, pressure and temperature varied between the different faults. We therefore conclude that the type of fault rock here depended on the host rock lithology, and that lithology alone accounts for why some faults developed a hydrothermal breccia, some cataclasite, and some a fine grained black fault rock. Consequently, based on the assumption that fault rocks reflect specific slip styles, lithology was also the main control on different fault slip styles in this area at the time of strike-slip fault activity. Whereas fine grained black fault rock is inferred to represent high stress events, hydrothermal breccia is rather related to events involving fluid pressure in excess of the least stress. Jasper-bearing cataclasites may represent faults that experienced dynamic weakening as seen

  15. Gamma Band Activity in the RAS-intracellular mechanisms

    PubMed Central

    Garcia-Rill, E.; Kezunovic, N.; D’Onofrio, S.; Luster, B.; Hyde, J.; Bisagno, V.; Urbano, F.J.

    2014-01-01

    Gamma band activity participates in sensory perception, problem solving, and memory. This review considers recent evidence showing that cells in the reticular activating system (RAS) exhibit gamma band activity, and describes the intrinsic membrane properties behind such manifestation. Specifically, we discuss how cells in the mesopontine pedunculopontine nucleus (PPN), intralaminar parafascicular nucleus (Pf), and pontine Subcoeruleus nucleus dorsalis (SubCD) all fire in the gamma band range when maximally activated, but no higher. The mechanisms involve high threshold, voltage-dependent P/Q-type calcium channels or sodium-dependent subthreshold oscillations. Rather than participating in the temporal binding of sensory events as in the cortex, gamma band activity in the RAS may participate in the processes of preconscious awareness, and provide the essential stream of information for the formulation of many of our actions. We address three necessary next steps resulting from these discoveries, an intracellular mechanism responsible for maintaining gamma band activity based on persistent G-protein activation, separate intracellular pathways that differentiate between gamma band activity during waking vs during REM sleep, and an intracellular mechanism responsible for the dysregulation in gamma band activity in schizophrenia. These findings open several promising research avenues that have not been thoroughly explored. What are the effects of sleep or REM sleep deprivation on these RAS mechanisms? Are these mechanisms involved in memory processing during waking and/or during REM sleep? Does gamma band processing differ during waking vs REM sleep after sleep or REM sleep deprivation? PMID:24309750

  16. Science Rocks!

    ERIC Educational Resources Information Center

    Prestwich, Dorothy; Sumrall, Joseph; Chessin, Debby A.

    2010-01-01

    It all began one Monday morning. Raymond could not wait to come to large group. In his hand, he held a chunk of white granite he had found. "Look at my beautiful rock!" he cried. The rock was passed around and examined by each student. "I wonder how rocks are made?" wondered one student. "Where do they come from?" asked another. At this moment, a…

  17. Rock flows

    NASA Technical Reports Server (NTRS)

    Matveyev, S. N.

    1986-01-01

    Rock flows are defined as forms of spontaneous mass movements, commonly found in mountainous countries, which have been studied very little. The article considers formations known as rock rivers, rock flows, boulder flows, boulder stria, gravel flows, rock seas, and rubble seas. It describes their genesis as seen from their morphological characteristics and presents a classification of these forms. This classification is based on the difference in the genesis of the rubbly matter and characterizes these forms of mass movement according to their source, drainage, and deposit areas.

  18. Geldanamycin anisimycins activate Rho and stimulate Rho- and ROCK-dependent actin stress fiber formation.

    PubMed

    Amiri, Anahita; Noei, Farahnaz; Feroz, Tahir; Lee, Jonathan M

    2007-09-01

    Heat shock protein 90 (Hsp90) is a member of the heat shock family of molecular chaperones that regulate protein conformation and activity. Hsp90 regulates multiple cell signaling pathways by controlling the abundance and activity of several important protein kinases and cell cycle-related proteins. In this report, we show that inhibition of Hsp90 by geldanamycin or its derivative, 17-allylamino-17-desmethoxygeldamycin, leads to activation of the Rho GTPase and a dramatic increase in actin stress fiber formation in human tumor cell lines. Inactivation of Rho prevents geldanamycin-induced actin reorganization. Hsp90 inactivation does not alter the appearance of filopodia or lamellipodia and tubulin architecture is not visibly perturbed. Our observations suggest that Hsp90 has an important and specific role in regulating Rho activity and Rho-dependent actin cytoskeleton remodeling.

  19. Mechanical Activation of Construction Binder Materials by Various Mills

    NASA Astrophysics Data System (ADS)

    Fediuk, R. S.

    2016-04-01

    The paper deals with the mechanical grinding down to the nano powder of construction materials. During mechanical activation a composite binder active molecules cement minerals occur in the destruction of the molecular defects in the areas of packaging and breaking metastable phase decompensation intermolecular forces. The process is accompanied by a change in the kinetics of hardening of portland cement. Mechanical processes during grinding mineral materials cause, along with the increase in their surface energy, increase the Gibbs energy of powders and, respectively, their chemical activity, which also contributes to the high adhesion strength when contacting them with binders. Thus, the set of measures for mechanical activation makes better use of the weight of components filled with cement systems and adjust their properties. At relatively low cost is possible to provide a spectacular and, importantly, easily repeatable results in a production environment.

  20. Integrated Experimental and Modeling Studies of Mineral Carbonation as a Mechanism for Permanent Carbon Sequestration in Mafic/Ultramafic Rocks

    SciTech Connect

    Wang, Zhengrong; Qiu, Lin; Zhang, Shuang; Bolton, Edward; Bercovici, David; Ague, Jay; Karato, Shun-Ichiro; Oristaglio, Michael; Zhu, Wen-Iu; Lisabeth, Harry; Johnson, Kevin

    2014-09-30

    A program of laboratory experiments, modeling and fieldwork was carried out at Yale University, University of Maryland, and University of Hawai‘i, under a DOE Award (DE-FE0004375) to study mineral carbonation as a practical method of geologic carbon sequestration. Mineral carbonation, also called carbon mineralization, is the conversion of (fluid) carbon dioxide into (solid) carbonate minerals in rocks, by way of naturally occurring chemical reactions. Mafic and ultramafic rocks, such as volcanic basalt, are natural candidates for carbonation, because the magnesium and iron silicate minerals in these rocks react with brines of dissolved carbon dioxide to form carbonate minerals. By trapping carbon dioxide (CO2) underground as a constituent of solid rock, carbonation of natural basalt formations would be a secure method of sequestering CO2 captured at power plants in efforts to mitigate climate change. Geochemical laboratory experiments at Yale, carried out in a batch reactor at 200°C and 150 bar (15 MPa), studied carbonation of the olivine mineral forsterite (Mg2SiO4) reacting with CO2 brines in the form of sodium bicarbonate (NaHCO3) solutions. The main carbonation product in these reactions is the carbonate mineral magnesite (MgCO3). A series of 32 runs varied the reaction time, the reactive surface area of olivine grains and powders, the concentration of the reacting fluid, and the starting ratio of fluid to olivine mass. These experiments were the first to study the rate of olivine carbonation under passive conditions approaching equilibrium. The results show that, in a simple batch reaction, olivine carbonation is fastest during the first 24 hours and then slows significantly and even reverses. A natural measure of the extent of carbonation is a quantity called the carbonation fraction, which compares the amount of carbon removed from solution, during a run, to the maximum amount

  1. Activation Mechanism of the Bacteroides fragilis Cysteine Peptidase, Fragipain.

    PubMed

    Herrou, Julien; Choi, Vivian M; Bubeck Wardenburg, Juliane; Crosson, Sean

    2016-07-26

    Enterotoxigenic Bacteroides fragilis produces a secreted metalloprotease known as B. fragilis toxin (BFT), which contributes to anaerobic sepsis, colitis, and colonic malignancy in mouse models of disease. A C11 family cysteine protease, fragipain (Fpn), directly activates BFT in the B. fragilis cell by removing the BFT prodomain. Fpn is itself a proenzyme and is autoactivated upon cleavage at an arginine residue in its activation loop. We have defined the proteolytic active site of Fpn, demonstrated that Fpn autoactivation can occur by an in trans loop cleavage mechanism, and characterized structural features of the Fpn activation loop that control peptidase activity against several substrates, including BFT. An arginine residue at the autocleavage site determines the fast activation kinetics of Fpn relative to the homologous C11 protease, PmC11, which is cleaved at lysine. Arginine to alanine substitution at the cleavage site ablated peptidase activity, as did partial truncation of the Fpn activation loop. However, complete truncation of the activation loop yielded an uncleaved, pro form of Fpn that was active as a peptidase against both Fpn and BFT substrates. Thus, Fpn can be transformed into an active peptidase in the absence of activation loop cleavage. This study provides insight into the mechanism of fragipain activation and, more generally, defines the role of the C11 activation loop in the control of peptidase activity and substrate specificity.

  2. Activity concentration of natural radionuclides and radon and thoron exhalation rates in rocks used as decorative wall coverings in Japan.

    PubMed

    Iwaoka, Kazuki; Hosoda, Masahiro; Tabe, Hiroyuki; Ishikawa, Tetsuo; Tokonami, Shinji; Yonehara, Hidenori

    2013-01-01

    In Japan, many dwellings have decorative wall coverings made from granite, andesite, tuff, gabbro, and marble. However, information regarding activity concentrations and radon (Rn) and thoron (Rn) exhalation rates for such rocks is very scarce. Therefore, samples of the granite, andesite, tuff, and marble that are used as wall coverings in Japan were collected from mining companies, and their activity concentrations and Rn and Rn exhalation rates were measured. Dose estimations for inhabitants living in houses built with these materials were also carried out. The activity concentration of natural radionuclides in all the materials was lower than the critical values described by the International Atomic Energy Agency (IAEA) (10,000 Bq kg for K and 1,000 Bq kg for all other radionuclides of natural origin). The maximum values of Rn and Rn mass exhalation rates for the granite samples were 0.12 and 430 mBq kg s, and those for the area exhalation rates were 1.8 and 6300 mBq m s, respectively; these values are higher than those for other samples. The maximum value of effective doses to inhabitants was 0.68 mSv y, which is lower than the intervention exemption level (1 mSv y) given in the International Commission on Radiological Protection (ICRP) Publication 82.

  3. Activity concentration of natural radionuclides and radon and thoron exhalation rates in rocks used as decorative wall coverings in Japan.

    PubMed

    Iwaoka, Kazuki; Hosoda, Masahiro; Tabe, Hiroyuki; Ishikawa, Tetsuo; Tokonami, Shinji; Yonehara, Hidenori

    2013-01-01

    In Japan, many dwellings have decorative wall coverings made from granite, andesite, tuff, gabbro, and marble. However, information regarding activity concentrations and radon (Rn) and thoron (Rn) exhalation rates for such rocks is very scarce. Therefore, samples of the granite, andesite, tuff, and marble that are used as wall coverings in Japan were collected from mining companies, and their activity concentrations and Rn and Rn exhalation rates were measured. Dose estimations for inhabitants living in houses built with these materials were also carried out. The activity concentration of natural radionuclides in all the materials was lower than the critical values described by the International Atomic Energy Agency (IAEA) (10,000 Bq kg for K and 1,000 Bq kg for all other radionuclides of natural origin). The maximum values of Rn and Rn mass exhalation rates for the granite samples were 0.12 and 430 mBq kg s, and those for the area exhalation rates were 1.8 and 6300 mBq m s, respectively; these values are higher than those for other samples. The maximum value of effective doses to inhabitants was 0.68 mSv y, which is lower than the intervention exemption level (1 mSv y) given in the International Commission on Radiological Protection (ICRP) Publication 82. PMID:23192085

  4. {delta}-ALAD activity variations in red blood cells in response to lead accumulation in rock doves (Columba livia)

    SciTech Connect

    Gonzalez, M.; Tejedor, M.C.

    1992-10-01

    The enzyme {delta}-aminolevulinic acid dehydratase ({delta}-ALAD, E.C. 4.2.1.24), catalyses the second step of the haeme biosynthetic pathway and is required to maintain the haemoglobin and cytochrome content in red cells. {delta}-ALAD is not only found in bone marrow cells, the major site of haeme synthesis, but also in circulating erythrocytes and other tissues. An inverse correlation was found between {delta}-ALAD activity in red blood cells and lead concentration in the blood. The degree of {delta}-ALAD inhibition in erythrocytes has been widely accepted as a standard bioassay to detect acute and chronic lead exposure in humans and in avians. The value of this parameter as an indicator for environmental lead has been often reported in doves and Scanlon. In lead-treated rats, an increase in {delta}-ALAD activity in bone marrow cells and in blood samples was shown by radioimmunoassay at 5 and 9 days after the treatment. Similarly, the amount of {delta}-ALAD seems to be more sensitive to lead in avian species than in mammals, the usefulness of blood {delta}-ALAD activity as an index of lead exposure has already been questioned by Hutton in the pigeon and by Jaffe et al. in humans. The present investigation studied the toxic effects of lead on rock dove red blood cell {delta}-ALAD activity in two situations: in doves treated with lead acetate in the laboratory and in doves exposed to the environment of Alcala de Henares. The final lead blood concentrations were lower in the environmental than in the laboratory doves. {delta}-ALAD activity in bone marrow cells and the relationships between lead accumulation and enzyme activity in red cells, are examined. 20 refs., 5 figs., 1 tab.

  5. Determination of thorium concentrations and activity ratios in silicate rocks by alpha spectrometry.

    PubMed

    dos Santos, R N; Marques, L S; Nicolai, S H A; Ribeiro, F B

    2004-01-01

    A detailed radiochemical procedure for alpha spectrometry measurements of thorium concentrations and of 230Th/232Th activity ratios in silicates is presented. The Th behaviour, during each step of the chemical process, was investigated by using a 234Th tracer, which is a gamma-ray emitter. The described chemical processing provides relatively high thorium yields, which varied between 56% and 88%, in the analysis of GB-1 (granite) and BB-1 (basalt) Brazilian geological standards. Also, the application of the established radiochemical method allowed a determination of both Th concentrations and activity ratios with high reproducibility, on the order of 2%. The estimation of the concentration result accuracy is also about 2%, which was calculated by using published data obtained from neutron activation analysis as reference values.

  6. Long Live Rock! Exploring Active Microbial Populations in North Pond Subsurface Basalt

    NASA Astrophysics Data System (ADS)

    Mills, H. J.; Lehne, J.

    2014-12-01

    Microbial life should be considered as an active source for subsurface alterations of crustal material. Over the past several decades, microbial populations have been qualitatively and quantitatively characterized in marine sediments from the near shore to gyre centers, from the surface to two kilometers below the surface. Recent exploration of the underlying basement has revealed bacterial populations within the basalt. Initial cultivation-based and in situ analysis of subsurface basalt has produced some structural identification of populations that have the potential to alter the crust. Within this study, we have advanced this understanding by characterizing the metabolically active fraction of these populations. A 16S rRNA gene transcript approach was conducted using high throughput sequencing on RNA extracted from breccia, glass basalts and ultramafic basalts of the western flank of the Mid-Atlantic Ridge. Previous research has shown that the fluid within the basement is oxic. As expected, populations associated with aerobic metabolism were detected. In addition, iron-utilizing populations were observed to be metabolically active within the basalt samples characterized. Future characterization will reveal overlap between previous studies to determine the total versus metabolically active populations.

  7. 'Tetl' Rock

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This image, taken by the panoramic camera on NASA's Mars Exploration Rover Spirit during the rover's trek through the 'Columbia Hills' at 'Gusev Crater,' shows the horizontally layered rock dubbed 'Tetl.' Scientists hope to investigate this rock in more detail, aiming to determine whether the rock's layering is volcanic or sedimentary in origin. If for some reason this particular rock is not favorably positioned for grinding and examination by the toolbox of instruments on the rover's robotic arm, Spirit will be within short reach of another similar rock, dubbed 'Coba.' Spirit took this image on its 264th martian day, or sol (Sept. 29, 2004). This is a false-color composite image generated from the panoramic camera's 750-, 530-, and 430-nanometer filters.

  8. Rocks of the Columbia Hills

    USGS Publications Warehouse

    Squyres, S. W.; Arvidson, R. E.; Blaney, D.L.; Clark, B. C.; Crumpler, L.; Farrand, W. H.; Gorevan, S.; Herkenhoff, K. E.; Hurowitz, J.; Kusack, A.; McSween, H.Y.; Ming, D. W.; Morris, R.V.; Ruff, S.W.; Wang, A.; Yen, A.

    2006-01-01

    The Mars Exploration Rover Spirit has identified five distinct rock types in the Columbia Hills of Gusev crater. Clovis Class rock is a poorly sorted clastic rock that has undergone substantial aqueous alteration. We interpret it to be aqueously altered ejecta deposits formed by impacts into basaltic materials. Wishstone Class rock is also a poorly sorted clastic rock that has a distinctive chemical composition that is high in Ti and P and low in Cr. Wishstone Class rock may be pyroclastic or impact in origin. Peace Class rock is a sedimentary material composed of ultramafic sand grains cemented by significant quantities of Mg- and Ca-sulfates. Peace Class rock may have formed when water briefly saturated the ultramafic sands and evaporated to allow precipitation of the sulfates. Watchtower Class rocks are similar chemically to Wishstone Class rocks and have undergone widely varying degrees of near-isochemical aqueous alteration. They may also be ejecta deposits, formed by impacts into Wishstone-rich materials and altered by small amounts of water. Backstay Class rocks are basalt/trachybasalt lavas that were emplaced in the Columbia Hills after the other rock classes were, either as impact ejecta or by localized volcanic activity. The geologic record preserved in the rocks of the Columbia Hills reveals a period very early in Martian history in which volcanic materials were widespread, impact was a dominant process, and water was commonly present. Copyright 2006 by the American Geophysical Union.

  9. Anthocyanidins inhibit activator protein 1 activity and cell transformation: structure-activity relationship and molecular mechanisms.

    PubMed

    Hou, De-Xing; Kai, Keiko; Li, Jian-Jian; Lin, Shigang; Terahara, Norihiko; Wakamatsu, Mika; Fujii, Makoto; Young, Mattew R; Colburn, Nancy

    2004-01-01

    Anthocyanins are the chemical components that give the intense color to many fruits and vegetables, such as blueberries, red cabbages and purple sweet potatoes. Extensive studies have indicated that anthocyanins have strong antioxidant activities. To investigate the mechanism of anthocyanidins as an anticancer food source, six kinds of anthocyanidins representing the aglycons of most anthocyanins, were used to examine their effects on tumor promotion in mouse JB6 cells, a validated model for screening cancer chemopreventive agents and elucidating the molecular mechanisms. Of the six anthocyanins tested, only those with an ortho-dihydroxyphenyl structure on the B-ring suppressed 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation and activator protein-1 transactivation, suggesting that the ortho-dihydroxyphenyl may contribute to the inhibitory action. Delphinidin, but not peonidin, blocked the phosphorylation of protein kinases in the extracellular signal-regulated protein kinase (ERK) pathway at early times and the c-Jun N-terminal kinase (JNK) signaling pathway at later times. p38 kinase was not inhibited by delphinidin. Furthermore, two mitogen-activated protein kinase (MAPK) specific inhibitors (SP600125 for JNK and UO126 for ERK) could specifically block the activation of JNK and ERK and cell transformation. Those results demonstrate that anthocyanidins contribute to the inhibition of tumorigenesis by blocking activation of the MAPK pathway. These findings provide the first molecular basis for the anticarcinogenic action of anthocyanidins. PMID:14514663

  10. Anthocyanidins inhibit activator protein 1 activity and cell transformation: structure-activity relationship and molecular mechanisms.

    PubMed

    Hou, De-Xing; Kai, Keiko; Li, Jian-Jian; Lin, Shigang; Terahara, Norihiko; Wakamatsu, Mika; Fujii, Makoto; Young, Mattew R; Colburn, Nancy

    2004-01-01

    Anthocyanins are the chemical components that give the intense color to many fruits and vegetables, such as blueberries, red cabbages and purple sweet potatoes. Extensive studies have indicated that anthocyanins have strong antioxidant activities. To investigate the mechanism of anthocyanidins as an anticancer food source, six kinds of anthocyanidins representing the aglycons of most anthocyanins, were used to examine their effects on tumor promotion in mouse JB6 cells, a validated model for screening cancer chemopreventive agents and elucidating the molecular mechanisms. Of the six anthocyanins tested, only those with an ortho-dihydroxyphenyl structure on the B-ring suppressed 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation and activator protein-1 transactivation, suggesting that the ortho-dihydroxyphenyl may contribute to the inhibitory action. Delphinidin, but not peonidin, blocked the phosphorylation of protein kinases in the extracellular signal-regulated protein kinase (ERK) pathway at early times and the c-Jun N-terminal kinase (JNK) signaling pathway at later times. p38 kinase was not inhibited by delphinidin. Furthermore, two mitogen-activated protein kinase (MAPK) specific inhibitors (SP600125 for JNK and UO126 for ERK) could specifically block the activation of JNK and ERK and cell transformation. Those results demonstrate that anthocyanidins contribute to the inhibition of tumorigenesis by blocking activation of the MAPK pathway. These findings provide the first molecular basis for the anticarcinogenic action of anthocyanidins.

  11. Tractor Mechanics: Learning Activity Packages 1-19.

    ERIC Educational Resources Information Center

    Clemson Univ., SC. Vocational Education Media Center.

    Learning activity packages are presented for teaching tractor mechanics. The first of two sections deals with miscellaneous tasks and contains learning activity packages on cleaning the tractor and receiving new tractor parts. Section 2 is concerned with maintaining and servicing the electrical system, and it includes the following learning…

  12. Calcineurin/NFAT Activation-Dependence of Leptin Synthesis and Vascular Growth in Response to Mechanical Stretch

    PubMed Central

    Soudani, Nadia; Ghantous, Crystal M.; Farhat, Zein; Shebaby, Wassim N.; Zibara, Kazem; Zeidan, Asad

    2016-01-01

    Background and Aims: Hypertension and obesity are important risk factors of cardiovascular disease. They are both associated with high leptin levels and have been shown to promote vascular hypertrophy, through the RhoA/ROCK and ERK1/2 phosphorylation. Calcineurin/NFAT activation also induces vascular hypertrophy by upregulating various genes. This study aimed to decipher whether a crosstalk exists between the RhoA/ROCK pathway, Ca2+/calcineurin/NFAT pathway, and ERK1/2 phosphorylation in the process of mechanical stretch-induced vascular smooth muscle cell (VSMC) hypertrophy and leptin synthesis. Methods and Results: Rat portal vein (RPV) organ culture was used to investigate the effect of mechanical stretch and exogenous leptin (3.1 nM) on VSMC hypertrophy and leptin synthesis. Results showed that stretching the RPV significantly upregulated leptin secretion, mRNA, and protein expression, which were inhibited by the calcium channel blocker nifedipine (10 μM), the selective calcineurin inhibitor FK506 (1 nM), and the ERK1/2 inhibitor PD98059 (1 μM). The transcription inhibitor actinomycin D (0.1 μM) and the translation inhibitor cycloheximide (1 mM) significantly decreased stretch-induced leptin protein expression. Mechanical stretch or leptin caused an increase in wet weight changes and protein synthesis, considered as hypertrophic markers, while they were inhibited by FK506 (0.1 nM; 1 nM). In addition, stretch or exogenous leptin significantly increased calcineurin activity and MCIP1 expression whereas leptin induced NFAT nuclear translocation in VSMCs. Moreover, in response to stretch or exogenous leptin, the Rho inhibitor C3 exoenzyme (30 ng/mL), the ROCK inhibitor Y-27632 (10 μM), and the actin depolymerization agents Latrunculin B (50 nM) and cytochalasin D (1 μM) reduced calcineurin activation and NFAT nuclear translocation. ERK1/2 phosphorylation was inhibited by FK506 and C3. Conclusions: Mechanical stretch-induced VSMC hypertrophy and leptin

  13. Changes in14c activity over time during vacuum distillation of carbon from rock pore water

    USGS Publications Warehouse

    Davidson, G.R.; Yang, I.C.

    1999-01-01

    The radiocarbon activity of carbon collected by vacuum distillation from a single partially saturated tuff began to decline after approximately 60% of the water and carbon had been extracted. Disproportionate changes in 14C activity and ??13C during distillation rule out simple isotopic fractionation as a causative explanation. Additional phenomena such as matrix diffusion and ion exclusion in micropores may play a role in altering the isotopic value of extracted carbon, but neither can fully account for the observed changes. The most plausible explanation is that distillation recovers carbon from an adsorbed phase that is depleted in 14C relative to DIC in the bulk pore water. ?? 1999 by the Arizona Board of Regents on behalf of the University of Arizona.

  14. Alkali-aggregate reactivity of typical siliceious glass and carbonate rocks in alkali-activated fly ash based geopolymers

    NASA Astrophysics Data System (ADS)

    Lu, Duyou; Liu, Yongdao; Zheng, Yanzeng; Xu, Zhongzi; Shen, Xiaodong

    2013-08-01

    For exploring the behaviour of alkali-aggregate reactivity (AAR) in alkali-activated geopolymeric materials and assessing the procedures for testing AAR in geopolymers, the expansion behaviour of fly ash based geopolymer mortars with pure silica glass and typical carbonate rocks were studied respectively by curing at various conditions, i.e. 23°C and 38°C with relative humidity over 95%, immersed in 1M NaOH solution at 80°C. Results show that, at various curing conditions, neither harmful ASR nor harmful ACR was observed in geopolymers with the criteria specified for OPC system. However, with the change of curing conditions, the geopolymer binder and reactive aggregates may experience different reaction processes leading to quite different dimensional changes, especially with additional alkalis and elevated temperatures. It suggests that high temperature with additional alkali for accelerating AAR in traditional OPC system may not appropriate for assessing the alkali-aggregate reactivity behaviour in geopolymers designed for normal conditions. On the other hand, it is hopeful to control the dimensional change of geopolymer mortar or concrete by selecting the type of aggregates and the appropriate curing conditions, thus changing the harmful AAR in OPC into beneficial AAR in geopolymers and other alkali-activated cementitious systems.

  15. Structural style, deformation mechanisms and paleodifferential stress along an exposed crustal section: constraints on the rheology of quartzofeldspathic rocks at supra- and infrastructural levels (Bohemian Massif)

    NASA Astrophysics Data System (ADS)

    Zulauf, G.

    2001-03-01

    Investigations of Variscan shortening of quartzofeldspathic rocks have been carried out along an exposed crustal section located in the western part of the Teplá Barrandian unit (Bohemian Massif). The study focuses on determining differential stress, deformation mechanisms and geometry of Cadomian metagreywackes and Cambrian granitoids which show different grade of Variscan Barrovian-type metamorphism ranging from the greenschist-/subgreenschist-facies boundary (semibrittle deformation regime) to the high-grade amphibolite facies. The semibrittle deformation regime ( T=ca. 300°C) is characterized by large-scale thrusting within a folding environment and by similar intensities of cataclasis, diffusive mass transfer, and crystal plasticity of quartz, the latter including low-temperature migration recrystallization. Within Cadomian quartz veins of the greenschist-facies part, dislocation creep of quartz operated at differential stresses ranging from ca. 70 MPa in the lowermost greenschist-facies to ca. 20 MPa in the uppermost greenschist-facies. These values have been derived by paleopiezometry using the grain size of recrystallized quartz. They are interpreted as upper bounds for the bulk stress because of intense diffusive mass transfer within the adjacent metagreywackes. A marking increase in recrystallized grain size of quartz occurs within the lower greenschist-facies, where low-temperature grain boundary migration is replaced by subgrain rotation. Thus, causion is needed when applying the recrystallized grain size paleopiezometer to this crustal level. Pressure solution of quartz and development of discrete crenulation cleavage was common in fine-grained metagreywackes of the lower greenschist-facies, whereas the upper greenschist-facies ( T=ca. 500-570°C) is characterized by increasing intensity of metamorphic reactions, grain coarsening and development of a schistosity. The entire greenschist-facies level forms a suprastructure where viscosity contrasts

  16. Art Rocks with Rock Art!

    ERIC Educational Resources Information Center

    Bickett, Marianne

    2011-01-01

    This article discusses rock art which was the very first "art." Rock art, such as the images created on the stone surfaces of the caves of Lascaux and Altimira, is the true origin of the canvas, paintbrush, and painting media. For there, within caverns deep in the earth, the first artists mixed animal fat, urine, and saliva with powdered minerals…

  17. Microbial populations and activities in the rhizoplane of rock-weathering desert plants. II. Growth promotion of cactus seedlings.

    PubMed

    Puente, M E; Li, C Y; Bashan, Y

    2004-09-01

    Four bacterial species isolated from the rhizoplane of cacti growing in bare lava rocks were assessed for growth promotion of giant cardon cactus seedlings (Pachycereus pringlei). These bacteria fixed N(2), dissolved P, weathered extrusive igneous rock, marble, and limestone, and significantly mobilized useful minerals, such as P, K, Mg, Mn, Fe, Cu, and Zn in rock minerals. Cardon cactus seeds inoculated with these bacteria were able to sprout and grow normally without added nutrients for at least 12 months in pulverized extrusive igneous rock (ancient lava flows) mixed with perlite. Cacti that were not inoculated grew less vigorously and some died. The amount of useful minerals (P, K, Fe, Mg) for plant growth extracted from the pulverized lava, measured after cultivation of inoculated plants, was significant. This study shows that rhizoplane bacteria isolated from rock-growing cacti promote growth of a cactus species, and can help supply essential minerals for a prolonged period of time.

  18. Microbial populations and activities in the rhizoplane of rock-weathering desert plants. II. Growth promotion of cactus seedlings.

    PubMed

    Puente, M E; Li, C Y; Bashan, Y

    2004-09-01

    Four bacterial species isolated from the rhizoplane of cacti growing in bare lava rocks were assessed for growth promotion of giant cardon cactus seedlings (Pachycereus pringlei). These bacteria fixed N(2), dissolved P, weathered extrusive igneous rock, marble, and limestone, and significantly mobilized useful minerals, such as P, K, Mg, Mn, Fe, Cu, and Zn in rock minerals. Cardon cactus seeds inoculated with these bacteria were able to sprout and grow normally without added nutrients for at least 12 months in pulverized extrusive igneous rock (ancient lava flows) mixed with perlite. Cacti that were not inoculated grew less vigorously and some died. The amount of useful minerals (P, K, Fe, Mg) for plant growth extracted from the pulverized lava, measured after cultivation of inoculated plants, was significant. This study shows that rhizoplane bacteria isolated from rock-growing cacti promote growth of a cactus species, and can help supply essential minerals for a prolonged period of time. PMID:15375736

  19. Yucca Mountain Project thermal and mechanical codes first benchmark exercise: Part 3, Jointed rock mass analysis; Yucca Mountain Site Characterization Project

    SciTech Connect

    Costin, L.S.; Bauer, S.J.

    1991-10-01

    Thermal and mechanical models for intact and jointed rock mass behavior are being developed, verified, and validated at Sandia National Laboratories for the Yucca Mountain Site Characterization Project. Benchmarking is an essential part of this effort and is one of the tools used to demonstrate verification of engineering software used to solve thermomechanical problems. This report presents the results of the third (and final) phase of the first thermomechanical benchmark exercise. In the first phase of this exercise, nonlinear heat conduction code were used to solve the thermal portion of the benchmark problem. The results from the thermal analysis were then used as input to the second and third phases of the exercise, which consisted of solving the structural portion of the benchmark problem. In the second phase of the exercise, a linear elastic rock mass model was used. In the third phase of the exercise, two different nonlinear jointed rock mass models were used to solve the thermostructural problem. Both models, the Sandia compliant joint model and the RE/SPEC joint empirical model, explicitly incorporate the effect of the joints on the response of the continuum. Three different structural codes, JAC, SANCHO, and SPECTROM-31, were used with the above models in the third phase of the study. Each model was implemented in two different codes so that direct comparisons of results from each model could be made. The results submitted by the participants showed that the finite element solutions using each model were in reasonable agreement. Some consistent differences between the solutions using the two different models were noted but are not considered important to verification of the codes. 9 refs., 18 figs., 8 tabs.

  20. Terby's Rocks

    NASA Technical Reports Server (NTRS)

    2006-01-01

    27 January 2006 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows some of the light-toned, layered, sedimentary rock outcrops in northern Terby Crater. Terby is located along the north edge of Hellas Planitia. The sedimentary rocks might have been deposited in a greater, Hellas-filling sea -- or not. Today, the rocks are partly covered by dark-toned sediment and debris.

    Location near: 27.2oS, 285.3oW Image width: 3 km (1.9 mi) Illumination from: upper left Season: Southern Summer

  1. Mechanism of phenol adsorption onto electro-activated carbon granules.

    PubMed

    Lounici, H; Aioueche, F; Belhocine, D; Drouiche, M; Pauss, A; Mameri, N

    2004-01-01

    The main purpose of this paper is to determine the mechanisms which govern the adsorption of the phenol onto electro-activated carbon granules. This new activation technique allowed an increase of the performance of the adsorbent. Two models were utilised to understand the improvement in the performance of electroactivated carbon granules. The first, a simple external resistance model based on film resistance, gave acceptable predictions, with an error of less than 15%, between the theoretical results and experimental data independent of the activation potential and phenol initial concentration. The second linear model, based on diffusion phenomena, was more representative in describing the experiment than the first model. It was observed that the electro-activation method did not change the mechanism which governs phenol adsorption onto granular carbon. Indeed, the same mathematical model based on diffusion phenomena made it possible to predict with a very low error (less than 5%) the experimental data obtained for the favourable activation potential, without activation potential and with an unfavourable activation potential. The electro-activation technique makes it possible to increase the number of active sites that improve the performance of the electro-activated granular carbon compared with conventional granular activated carbon.

  2. Application of flexure structures to active and adaptive opto-mechanical mechanisms

    NASA Astrophysics Data System (ADS)

    Zago, Lorenzo; Genequand, Pierre M.; Kjelberg, Ivar; Morschel, Joseph

    1997-03-01

    Active and adaptive structures, also commonly called 'smart' structures, combine in one integrated system various functions such as load carrying and structural function, mechanical (cinematic) functions, sensing, control and actuating. Originally developed for high accuracy opto-mechanical applications, CSEM's technology of flexure structures and flexible mechanisms is particularly suited to solve many structural and mechanical issues found in such active/adaptive mechanisms. The paper illustrates some recent flexure structures developments at CSEM and outlines the comprehensive know-how involved in this technology. This comprises in particular the elaboration of optimal design guidelines, related to the geometry, kinematics and dynamics issues (for instance, the minimization of spurious high frequency effects), the evaluation and predictability of all performance quantities relevant to the utilization of flexure structures in space (reliability, fatigue, static and dynamic modeling, etc.). material issues and manufacturing procedures.

  3. Active vibration control using mechanical and electrical analogies

    NASA Astrophysics Data System (ADS)

    Torres-Perez, A.; Hassan, A.; Kaczmarczyk, S.; Picton, P.

    2016-05-01

    Mechanical-electrical analogous circuit models are widely used in electromechanical system design as they represent the function of a coupled electrical and mechanical system using an equivalent electrical system. This research uses electrical circuits to establish a discussion of simple active vibration control principles using two scenarios: an active vibration isolation system and an active dynamic vibration absorber (DVA) using a voice coil motor (VCM) actuator. Active control laws such as gain scheduling are intuitively explained using circuit analysis techniques. Active vibration control approaches are typically constraint by electrical power requirements. The electrical analogous is a fast approach for specifying power requirements on the experimental test platform which is based on a vibration shaker that provides the based excitation required for the single Degree- of-Freedom (1DoF) vibration model under study.

  4. Cellular and Molecular Mechanisms Underpinning Macrophage Activation during Remyelination

    PubMed Central

    Lloyd, Amy F.; Miron, Veronique E.

    2016-01-01

    Remyelination is an example of central nervous system (CNS) regeneration, whereby myelin is restored around demyelinated axons, re-establishing saltatory conduction and trophic/metabolic support. In progressive multiple sclerosis, remyelination is limited or fails altogether which is considered to contribute to axonal damage/loss and consequent disability. Macrophages have critical roles in both CNS damage and regeneration, such as remyelination. This diverse range in functions reflects the ability of macrophages to acquire tissue microenvironment-specific activation states. This activation is dynamically regulated during efficient regeneration, with a switch from pro-inflammatory to inflammation-resolution/pro-regenerative phenotypes. Although, some molecules and pathways have been implicated in the dynamic activation of macrophages, such as NFκB, the cellular and molecular mechanisms underpinning plasticity of macrophage activation are unclear. Identifying mechanisms regulating macrophage activation to pro-regenerative phenotypes may lead to novel therapeutic strategies to promote remyelination in multiple sclerosis. PMID:27446913

  5. Biological mechanisms of physical activity in preventing cognitive decline.

    PubMed

    Lista, I; Sorrentino, G

    2010-05-01

    In order to guarantee better conditions for competition, the nervous system has developed not only mechanisms controlling muscle effectors, but also retrograde systems that, starting from peripheral structures, may influence brain functions. Under such perspective, physical activity could play an important role in influencing cognitive brain functions including learning and memory. The results of epidemiological studies (cross-sectional, prospective and retrospective) support a positive relationship between cognition and physical activities. Recent meta-analysis confirmed a significant effect of exercise on cognitive functions. However, the biological mechanisms that underlie such beneficial effects are still to be completely elucidated. They include supramolecular mechanisms (e.g. neurogenesis, synaptogenesis, and angiogenesis) which, in turn, are controlled by molecular mechanisms, such as BDNF, IGF-1, hormone and second messengers.

  6. Prolonged rock climbing activity induces structural changes in cerebellum and parietal lobe.

    PubMed

    Di Paola, Margherita; Caltagirone, Carlo; Petrosini, Laura

    2013-10-01

    This article analyzes whether climbing, a motor activity featured by upward movements by using both feet and hands, generation of new strategies of motor control, maintenance of not stable equilibrium and adoption of long-lasting quadrupedal posture, is able to modify specific brain areas. MRI data of 10 word-class mountain climbers (MC) and 10 age-matched controls, with no climbing experience were acquired. Combining region-of-interest analyses and voxel-based morphometry we investigated cerebellar volumes and correlation between cerebellum and whole cerebral gray matter. In comparison to controls, world-class MC showed significantly larger vermian lobules I-V volumes, with no significant difference in other cerebellar vermian lobules or hemispheres. The cerebellar enlargement was associated with an enlargement of right medial posterior parietal area. The specific features of the motor climbing skills perfectly fit with the plastic anatomical changes we found. The enlargement of the vermian lobules I-V seems to be related to highly dexterous hand movements and to eye-hand coordination in the detection of and correction of visuomotor errors. The concomitant enlargement of the parietal area is related to parallel work in predicting sensory consequences of action to make movement corrections. Motor control and sensory-motor prediction of actions make the difference between survive or not at extreme altitude. PMID:22522914

  7. Rock Garden

    NASA Technical Reports Server (NTRS)

    1997-01-01

    This false color composite image of the Rock Garden shows the rocks 'Shark' and 'Half Dome' at upper left and middle, respectively. Between these two large rocks is a smaller rock (about 0.20 m wide, 0.10 m high, and 6.33 m from the Lander) that was observed close-up with the Sojourner rover (see PIA00989).

    Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

  8. Mechanisms of Activation of Voltage-Gated Potassium Channels

    PubMed Central

    Grizel, A. V.; Glukhov, G. S.; Sokolova, O. S.

    2014-01-01

    Voltage-gated potassium ion channels (Kv) play an important role in a variety of cellular processes, including the functioning of excitable cells, regulation of apoptosis, cell growth and differentiation, the release of neurotransmitters and hormones, maintenance of cardiac activity, etc. Failure in the functioning of Kv channels leads to severe genetic disorders and the development of tumors, including malignant ones. Understanding the mechanisms underlying Kv channels functioning is a key factor in determining the cause of the diseases associated with mutations in the channels, and in the search for new drugs. The mechanism of activation of the channels is a topic of ongoing debate, and a consensus on the issue has not yet been reached. This review discusses the key stages in studying the mechanisms of functioning of Kv channels and describes the basic models of their activation known to date. PMID:25558391

  9. CONDENSED MATTER: STRUCTURE, MECHANICAL AND THERMAL PROPERTIES: Synchronization of Local Oscillations in a Spatial Rock-Scissors-Paper Game Model

    NASA Astrophysics Data System (ADS)

    Sun, Rong-Sheng; Hua, Da-Yin

    2009-08-01

    We study a spatial rock-scissors-paper model in a square lattice and a quenched small-world network. The system exhibits a global oscillation in the quenched small-world network, but the oscillation disappears in the square lattice. We find that there is a local oscillation in the square lattice the same as in the quenched small-world network. We define (where di is the density of a kind of species and langledirangle is the average value) as the variance of the oscillation amplitude in a certain local patch. It is found that s decays in a power law with an increase of the local patch size R in the square lattice σ propto R-δ, but it remains constant with an increase of the patch size in the quenched small-world network. We can speculate that in the square lattice, superposition between the local oscillations in different patches leads to global stabilization, while in the quenched small-world network, long-range interactions can synchronize the local oscillations, and their coherence results in the global oscillation.

  10. Processes, mechanisms, parameters, and modeling approaches for partially saturated flow in soil and rock media; Yucca Mountain Site Characterization Project

    SciTech Connect

    Wang, J.S.Y.; Narasimhan, T.N.

    1993-06-01

    This report discusses conceptual models and mathematical equations, analyzes distributions and correlations among hydrological parameters of soils and tuff, introduces new path integration approaches, and outlines scaling procedures to model potential-driven fluid flow in heterogeneous media. To properly model the transition from fracture-dominated flow under saturated conditions to matrix-dominated flow under partially saturated conditions, characteristic curves and permeability functions for fractures and matrix need to be improved and validated. Couplings from two-phase flow, heat transfer, solute transport, and rock deformation to liquid flow are also important. For stochastic modeling of alternating units of welded and nonwelded tuff or formations bounded by fault zones, correlations and constraints on average values of saturated permeability and air entry scaling factor between different units need to be imposed to avoid unlikely combinations of parameters and predictions. Large-scale simulations require efficient and verifiable numerical algorithms. New path integration approaches based on postulates of minimum work and mass conservation to solve flow geometry and potential distribution simultaneously are introduced. This verifiable integral approach, together with fractal scaling procedures to generate statistical realizations with parameter distribution, correlation, and scaling taken into account, can be used to quantify uncertainties and generate the cumulative distribution function for groundwater travel times.

  11. 'Lutefisk' Rock

    NASA Technical Reports Server (NTRS)

    2004-01-01

    [figure removed for brevity, see original site] Figure 1

    NASA's Mars Exploration Rover Spirit used its panoramic camera to take this image of a rock called 'Lutefisk' on the rover's 286th martian day (Oct. 22, 2004). The surface of the rock is studded with rounded granules of apparently more-resistant material up to several millimeters (0.1 inch) or more across. The visible portion of Lutefisk is about 25 centimeters (10 inches) across.

  12. Innovative Application of Mechanical Activation for Rare Earth Elements Recovering: Process Optimization and Mechanism Exploration.

    PubMed

    Tan, Quanyin; Deng, Chao; Li, Jinhui

    2016-01-01

    With the rapidly expanding use of fluorescent lamps (FLs) and increasing interest in conservation and sustainable utilization of critical metals such as rare earth elements (REEs), the recovering of REEs from phosphors in waste FLs is becoming a critical environmental and economic issue. To effectively recycle REEs with metallurgical methods, mechanical activation by ball milling was introduced to pretreat the waste phosphors. This current study put the emphasis on the mechanical activation and leaching processes for REEs, and explored the feasibility of the method from both theoretical and practical standpoints. Results showed physicochemical changes of structural destruction and particle size reduction after mechanical activation, leading to the easy dissolution of REEs in the activated samples. Under optimal conditions, dissolution yields of 89.4%, 93.1% and 94.6% for Tb, Eu and Y, respectively, were achieved from activated waste phosphors using hydrochloric acid as the dissolution agent. The shrinking core model proved to be the most applicable for the leaching procedure, with an apparent activation energy of 10.96 ± 2.79 kJ/mol. This novel process indicates that mechanical activation is an efficient method for recovering REEs from waste phosphors, and it has promising potential for REE recovery with low cost and high efficiency. PMID:26819083

  13. Innovative Application of Mechanical Activation for Rare Earth Elements Recovering: Process Optimization and Mechanism Exploration

    NASA Astrophysics Data System (ADS)

    Tan, Quanyin; Deng, Chao; Li, Jinhui

    2016-01-01

    With the rapidly expanding use of fluorescent lamps (FLs) and increasing interest in conservation and sustainable utilization of critical metals such as rare earth elements (REEs), the recovering of REEs from phosphors in waste FLs is becoming a critical environmental and economic issue. To effectively recycle REEs with metallurgical methods, mechanical activation by ball milling was introduced to pretreat the waste phosphors. This current study put the emphasis on the mechanical activation and leaching processes for REEs, and explored the feasibility of the method from both theoretical and practical standpoints. Results showed physicochemical changes of structural destruction and particle size reduction after mechanical activation, leading to the easy dissolution of REEs in the activated samples. Under optimal conditions, dissolution yields of 89.4%, 93.1% and 94.6% for Tb, Eu and Y, respectively, were achieved from activated waste phosphors using hydrochloric acid as the dissolution agent. The shrinking core model proved to be the most applicable for the leaching procedure, with an apparent activation energy of 10.96 ± 2.79 kJ/mol. This novel process indicates that mechanical activation is an efficient method for recovering REEs from waste phosphors, and it has promising potential for REE recovery with low cost and high efficiency.

  14. Innovative Application of Mechanical Activation for Rare Earth Elements Recovering: Process Optimization and Mechanism Exploration.

    PubMed

    Tan, Quanyin; Deng, Chao; Li, Jinhui

    2016-01-28

    With the rapidly expanding use of fluorescent lamps (FLs) and increasing interest in conservation and sustainable utilization of critical metals such as rare earth elements (REEs), the recovering of REEs from phosphors in waste FLs is becoming a critical environmental and economic issue. To effectively recycle REEs with metallurgical methods, mechanical activation by ball milling was introduced to pretreat the waste phosphors. This current study put the emphasis on the mechanical activation and leaching processes for REEs, and explored the feasibility of the method from both theoretical and practical standpoints. Results showed physicochemical changes of structural destruction and particle size reduction after mechanical activation, leading to the easy dissolution of REEs in the activated samples. Under optimal conditions, dissolution yields of 89.4%, 93.1% and 94.6% for Tb, Eu and Y, respectively, were achieved from activated waste phosphors using hydrochloric acid as the dissolution agent. The shrinking core model proved to be the most applicable for the leaching procedure, with an apparent activation energy of 10.96 ± 2.79 kJ/mol. This novel process indicates that mechanical activation is an efficient method for recovering REEs from waste phosphors, and it has promising potential for REE recovery with low cost and high efficiency.

  15. Innovative Application of Mechanical Activation for Rare Earth Elements Recovering: Process Optimization and Mechanism Exploration

    PubMed Central

    Tan, Quanyin; Deng, Chao; Li, Jinhui

    2016-01-01

    With the rapidly expanding use of fluorescent lamps (FLs) and increasing interest in conservation and sustainable utilization of critical metals such as rare earth elements (REEs), the recovering of REEs from phosphors in waste FLs is becoming a critical environmental and economic issue. To effectively recycle REEs with metallurgical methods, mechanical activation by ball milling was introduced to pretreat the waste phosphors. This current study put the emphasis on the mechanical activation and leaching processes for REEs, and explored the feasibility of the method from both theoretical and practical standpoints. Results showed physicochemical changes of structural destruction and particle size reduction after mechanical activation, leading to the easy dissolution of REEs in the activated samples. Under optimal conditions, dissolution yields of 89.4%, 93.1% and 94.6% for Tb, Eu and Y, respectively, were achieved from activated waste phosphors using hydrochloric acid as the dissolution agent. The shrinking core model proved to be the most applicable for the leaching procedure, with an apparent activation energy of 10.96 ± 2.79 kJ/mol. This novel process indicates that mechanical activation is an efficient method for recovering REEs from waste phosphors, and it has promising potential for REE recovery with low cost and high efficiency. PMID:26819083

  16. Modulation of bone remodeling via mechanically activated ion channels

    NASA Technical Reports Server (NTRS)

    Duncan, Randall L. (Principal Investigator)

    1996-01-01

    A critical factor in the maintenance of bone mass is the physical forces imposed upon the skeleton. Removal of these forces, such as in a weightless environment, results in a rapid loss of bone, whereas application of exogenous mechanical strain has been shown to increase bone formation. Numerous flight and ground-based experiments indicate that the osteoblast is the key bone cell influenced by mechanical stimulation. Aside from early transient fluctuations in response to unloading, osteoclast number and activity seem unaffected by removal of strain. However, bone formation is drastically reduced in weightlessness and osteoblasts respond to mechanical strain with an increase in the activity of a number of second messenger pathways resulting in increased anabolic activity. Unfortunately, the mechanism by which the osteoblast converts physical stimuli into a biochemical message, a process we have termed biochemical coupling, remains elusive. Prior to the application of this grant, we had characterized a mechanosensitive, cation nonselective channel (SA-cat) in osteoblast-like osteosarcoma cells that we proposed is the initial signalling mechanism for mechanotransduction. During the execution of this grant, we have made considerable progress to further characterize this channel as well as to determine its role in the osteoblastic response to mechanical strain. To achieve these goals, we combined electrophysiologic techniques with cellular and molecular biology methods to examine the role of these channels in the normal function of the osteoblast in vitro.

  17. Neutron activation and other analytical data for plutonic rocks from North America and Africa. National Uranium Resource Evaluation

    SciTech Connect

    Price, V.; Fay, W.M.; Cook, J.R.

    1982-09-01

    The objective of this report is to retrieve the elements of an analytical study of granites and associated other plutonic rocks which was begun as a part of the U.S. Department of Energy's National Uranium Resource Evaluation (NURE) program. A discussion of the Savannah River Laboratory (SRL) neutron activation analysis system is given so that a user will understand the linmitations of the data. Enough information is given so that an experienced geochemist can clean up the data set to the extent required by any project. The data are generally good as they are presented. It is intended that the data be read from a magnetic tape written to accompany this report. Microfiche tables of the data follow the text. These tables were prepared from data on the tape, and programs which will read the tape are presented in the section THE DATA TAPE. It is our intent to write a later paper which will include a thoroughly scrubbed data set and a technical discussion of results of the study. 1 figure.

  18. Rheological and geodynamic controls on the mechanisms of subduction and HP/UHP exhumation of crustal rocks during continental collision: Insights from numerical models

    NASA Astrophysics Data System (ADS)

    Burov, Evgene; Francois, Thomas; Agard, Philippe; Le Pourhiet, Laetitia; Meyer, Bertrand; Tirel, Celine; Lebedev, Sergei; Yamato, Philippe; Brun, Jean-Pierre

    2014-09-01

    While subduction of crustal rocks is increasingly accepted as a common scenario inherent to convergent processes involving continental plates and micro-continents, its occurrence in each particular context, as well as its specific mechanisms and conditions is still debated. The presence of ultra-high pressure(UHP) terranes is often interpreted as a strong evidence for continental subduction (subduction of continental crust) since the latter is seen as the most viable mechanism of their burial to UHP depths, yet if one admits nearly lithostatic pressure conditions in the subduction interface (or "channel"). The presumed links of continental subduction to exhumation of high- and ultra-high-pressure (HP/UHP) units also remain a subject of controversy despite the fact that recent physically consistent thermo-mechanical numerical models of convergent processes suggest that subduction can create specific mechanisms for UHP exhumation. We hence review and explore possible scenarios of subduction of continental crust, and their relation to exhumation of HP and UHP rocks as inferred from last generation of thermo-mechanical numerical models accounting for thermo-rheological complexity and structural diversity of the continental lithosphere. The inferences from these models are matched with the petrology data, in particular, with P-T-t paths, allowing for better understanding of subtleties of both subduction and burial/exhumation mechanisms. Numerical models suggest that exhumation and continental subduction are widespread but usually transient processes that last for less than 5-10 Myr, while long-lasting (> 10-15 Myr) subduction can take place only in rare cases of fast convergence of cold strong lithospheres (e.g. India). The models also show that tectonic heritage can play a special role in subduction/exhumation processes. In particular, when thicker continental terrains are embedded in subducting oceanic plate, exhumation of UHP terranes results in the formation of

  19. Small molecules reveal an alternative mechanism of Bax activation

    PubMed Central

    Brahmbhatt, Hetal; Uehling, David; Al-awar, Rima; Leber, Brian; Andrews, David

    2016-01-01

    The pro-apoptotic protein Bax commits a cell to death by permeabilizing the mitochondrial outer membrane (MOM). To obtain small-molecule probes for elucidating the molecular mechanism(s) of Bax activation, we screened for compounds that induced Bax-mediated liposome permeabilization. We identified five structurally different small molecules that promoted both Bax targeting to and oligomerization at membranes. All five compounds initiated Bax oligomerization in the absence of membranes by a mechanism unlike Bax activation by Bcl-2 homology 3 domain (BH3) proteins. Some of the compounds induced Bax/Bak-dependent apoptosis in cells. Activation of Bax by the most active compound was poorly inhibited by the anti-apoptotic protein Bcl-XL and requires a cysteine residue at position 126 of Bax that is not required for activation by BH3 proteins. Our results reveal a novel pathway for Bax activation independent of pro-apoptotic BH3 proteins that may have important implications for the regulation of Bax activity in cells. PMID:26916338

  20. The mechanical and tribological properties of UHMWPE loaded ALN after mechanical activation for joint replacements.

    PubMed

    Gong, Kemeng; Qu, Shuxin; Liu, Yumei; Wang, Jing; Zhang, Yongchao; Jiang, Chongxi; Shen, Ru

    2016-08-01

    Ultra-high molecular weight polyethylene (UHMWPE) loaded with alendronate sodium (ALN) has tremendous potential as an orthopeadic biomaterial for joint replacements. However, poor mechanical and tribological properties of UHMWPE-ALN are still obstacle for further application. The purpose of this study was to investigate the effect and mechanism of mechanical activation on mechanical and tribological properties of 1wt% ALN-loaded UHMWPE (UHMWPE-ALN-ma). In this study, tensile test, small punch test and reciprocating sliding wear test were applied to characterize the mechanical and tribological properties of UHMWPE-ALN-ma. Scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and Fourier transform infrared spectroscopy (FTIR) were employed to characterize UHMWPE-ALN-ma. Tensile test and small punch test showed that Young׳s modulus, tensile strength and work-to-failure (WTF) of UHMWPE-ALN-ma increased significantly compared to those of UHMWPE-ALN. The friction coefficients and wear factors of UHMWPE-ALN-ma both decreased significantly compared to those of UHMWPE-ALN. Mechanical activation obviously reduced type 1 (void) and type 2 (the disconnected and dislocated machining marks) fusion defects of UHMWPE-ALN-ma, which were revealed by SEM images of freeze fracture surfaces after etching and lateral surfaces of specimens after extension to fracture, respectively. It was attributed to peeled-off layers and chain scission of molecular chains of UHMWPE particles after mechanical activation, which were revealed by SEM images and FTIR spectra of UHMWPE-ALN-ma and UHMWPE-ALN, respectively. Moreover, EDS spectra revealed the more homogeneous distribution of ALN in UHMWPE-ALN-ma compared to that of UHMWPE-ALN. The present results showed that mechanical activation was a potential strategy to improve mechanical and tribological properties of UHMWPE-ALN-ma as an orthopeadic biomaterial for joint replacements. PMID:27104932

  1. The mechanical and tribological properties of UHMWPE loaded ALN after mechanical activation for joint replacements.

    PubMed

    Gong, Kemeng; Qu, Shuxin; Liu, Yumei; Wang, Jing; Zhang, Yongchao; Jiang, Chongxi; Shen, Ru

    2016-08-01

    Ultra-high molecular weight polyethylene (UHMWPE) loaded with alendronate sodium (ALN) has tremendous potential as an orthopeadic biomaterial for joint replacements. However, poor mechanical and tribological properties of UHMWPE-ALN are still obstacle for further application. The purpose of this study was to investigate the effect and mechanism of mechanical activation on mechanical and tribological properties of 1wt% ALN-loaded UHMWPE (UHMWPE-ALN-ma). In this study, tensile test, small punch test and reciprocating sliding wear test were applied to characterize the mechanical and tribological properties of UHMWPE-ALN-ma. Scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and Fourier transform infrared spectroscopy (FTIR) were employed to characterize UHMWPE-ALN-ma. Tensile test and small punch test showed that Young׳s modulus, tensile strength and work-to-failure (WTF) of UHMWPE-ALN-ma increased significantly compared to those of UHMWPE-ALN. The friction coefficients and wear factors of UHMWPE-ALN-ma both decreased significantly compared to those of UHMWPE-ALN. Mechanical activation obviously reduced type 1 (void) and type 2 (the disconnected and dislocated machining marks) fusion defects of UHMWPE-ALN-ma, which were revealed by SEM images of freeze fracture surfaces after etching and lateral surfaces of specimens after extension to fracture, respectively. It was attributed to peeled-off layers and chain scission of molecular chains of UHMWPE particles after mechanical activation, which were revealed by SEM images and FTIR spectra of UHMWPE-ALN-ma and UHMWPE-ALN, respectively. Moreover, EDS spectra revealed the more homogeneous distribution of ALN in UHMWPE-ALN-ma compared to that of UHMWPE-ALN. The present results showed that mechanical activation was a potential strategy to improve mechanical and tribological properties of UHMWPE-ALN-ma as an orthopeadic biomaterial for joint replacements.

  2. The Antiviral Activities and Mechanisms of Marine Polysaccharides: An Overview

    PubMed Central

    Wang, Wei; Wang, Shi-Xin; Guan, Hua-Shi

    2012-01-01

    Recently, the studies on the antiviral activities of marine natural products, especially marine polysaccharides, are attracting more and more attention all over the world. Marine-derived polysaccharides and their lower molecular weight oligosaccharide derivatives have been shown to possess a variety of antiviral activities. This paper will review the recent progress in research on the antiviral activities and the mechanisms of these polysaccharides obtained from marine organisms. In particular, it will provide an update on the antiviral actions of the sulfated polysaccharides derived from marine algae including carrageenans, alginates, and fucans, relating to their structure features and the structure–activity relationships. In addition, the recent findings on the different mechanisms of antiviral actions of marine polysaccharides and their potential for therapeutic application will also be summarized in detail. PMID:23235364

  3. Deep evolutionary conservation of an intramolecular protein kinase activation mechanism.

    PubMed

    Han, Jingfen; Miranda-Saavedra, Diego; Luebbering, Nathan; Singh, Aman; Sibbet, Gary; Ferguson, Michael A J; Cleghon, Vaughn

    2012-01-01

    DYRK-family kinases employ an intramolecular mechanism to autophosphorylate a critical tyrosine residue in the activation loop. Once phosphorylated, DYRKs lose tyrosine kinase activity and function as serine/threonine kinases. DYRKs have been characterized in organisms from yeast to human; however, all entities belong to the Unikont supergroup, only one of five eukaryotic supergroups. To assess the evolutionary age and conservation of the DYRK intramolecular kinase-activation mechanism, we surveyed 21 genomes representing four of the five eukaryotic supergroups for the presence of DYRKs. We also analyzed the activation mechanism of the sole DYRK (class 2 DYRK) present in Trypanosoma brucei (TbDYRK2), a member of the excavate supergroup and separated from Drosophila by ∼850 million years. Bioinformatics showed the DYRKs clustering into five known subfamilies, class 1, class 2, Yaks, HIPKs and Prp4s. Only class 2 DYRKs were present in all four supergroups. These diverse class 2 DYRKs also exhibited conservation of N-terminal NAPA regions located outside of the kinase domain, and were shown to have an essential role in activation loop autophosphorylation of Drosophila DmDYRK2. Class 2 TbDYRK2 required the activation loop tyrosine conserved in other DYRKs, the NAPA regions were critical for this autophosphorylation event, and the NAPA-regions of Trypanosoma and human DYRK2 complemented autophosphorylation by the kinase domain of DmDYRK2 in trans. Finally, sequential deletion analysis was used to further define the minimal region required for trans-complementation. Our analysis provides strong evidence that class 2 DYRKs were present in the primordial or root eukaryote, and suggest this subgroup may be the oldest, founding member of the DYRK family. The conservation of activation loop autophosphorylation demonstrates that kinase self-activation mechanisms are also primitive.

  4. Mechanisms of NOD-like receptor-associated inflammasome activation.

    PubMed

    Wen, Haitao; Miao, Edward A; Ting, Jenny P-Y

    2013-09-19

    A major function of a subfamily of NLR (nucleotide-binding domain, leucine-rich repeat containing, or NOD-like receptor) proteins is in inflammasome activation, which has been implicated in a multitude of disease models and human diseases. This work will highlight key progress in understanding the mechanisms that activate the best-studied NLRs (NLRP3, NLRC4, NAIP, and NLRP1) and in uncovering inflammasome NLRs. PMID:24054327

  5. Calc-alkaline mafic rocks of the Black Dyke Formation: Remnants of the final activity of a submerged Permian volcano

    SciTech Connect

    Blein, O.; Lapierre, H.; Pecher, A. ); Schweickert, R.A. . Dept. of Geological Science)

    1993-04-01

    The Permian Black Dyke Fm., which occurs as large tectonic slices within the Luning allochthon in the Excelsior Mountains, NV, forms an E-W trending anticline at Black Dyke Mountain. The 800-m thick stratigraphic succession consists of volcanic and pyroclastic rocks overlain conformably by volcaniclastic sediments. Along the northern limb of the anticline, the rocks consist of mafic porphyritic lavas, breccias, and graded and ungraded pyroclastic beds. The sedimentary unit consists of thick volcaniclastic turbidites overlain by conglomerates, sandstones, and mudstones. Along the southern limb of the anticline, the sequence is replaced by reworked breccia, tuffs, and sandstones. Mafic plutonic rocks occur as xenoliths in the lavas and breccias, and as coeval plugs intruding the section. Gabbros show cumulate or porphyritic textures and are composed of amph, cpx, and zoned plag. Their Ti/V (14.5--15) and Nb/Y (0.25--0.3) ratios fall in the range commonly found in calc-alkaline rocks. Diorite porphyry shows high Al[sub 2]O[sub 3], ZrO[sub 2], and REE abundances indicating that this rock is more fractionated. Basalts and andesites are plag-cpx-opx phyric. They often include glomeroporphyritic clots of cpx with amph coronas. Some rocks exhibit fluidal textures. Both volcanic and plutonic rocks show homogeneous geochemical features and similar crystallization sequences: Fe-Ti oxides---->plag---->opx + cpx----> brown zoned hbl, suggesting that they are cogenetic. Thus, the lower part of the Black Dyke Fm. likely represents the final products formed in a calc-alkaline magma chamber because pyroclastic rocks prevail over lava flows and abundant early crystal cumulates occur as plugs or as inclusions in the lavas and breccias.

  6. An Analytical Solution for Mechanical Responses Induced by Temperature and Air Pressure in a Lined Rock Cavern for Underground Compressed Air Energy Storage

    NASA Astrophysics Data System (ADS)

    Zhou, Shu-Wei; Xia, Cai-Chu; Du, Shi-Gui; Zhang, Ping-Yang; Zhou, Yu

    2015-03-01

    Mechanical responses induced by temperature and air pressure significantly affect the stability and durability of underground compressed air energy storage (CAES) in a lined rock cavern. An analytical solution for evaluating such responses is, thus, proposed in this paper. The lined cavern of interest consists of three layers, namely, a sealing layer, a concrete lining and the host rock. Governing equations for cavern temperature and air pressure, which involve heat transfer between the air and surrounding layers, are established first. Then, Laplace transform and superposition principle are applied to obtain the temperature around the lined cavern and the air pressure during the operational period. Afterwards, a thermo-elastic axisymmetrical model is used to analytically determine the stress and displacement variations induced by temperature and air pressure. The developments of temperature, displacement and stress during a typical operational cycle are discussed on the basis of the proposed approach. The approach is subsequently verified with a coupled compressed air and thermo-mechanical numerical simulation and by a previous study on temperature. Finally, the influence of temperature on total stress and displacement and the impact of the heat transfer coefficient are discussed. This paper shows that the temperature sharply fluctuates only on the sealing layer and the concrete lining. The resulting tensile hoop stresses on the sealing layer and concrete lining are considerably large in comparison with the initial air pressure. Moreover, temperature has a non-negligible effect on the lined cavern for underground compressed air storage. Meanwhile, temperature has a greater effect on hoop and longitudinal stress than on radial stress and displacement. In addition, the heat transfer coefficient affects the cavern stress to a higher degree than the displacement.

  7. Deriving mechanisms and thresholds for cliff retreat in soft-rock cliffs under changing climates: Rapidly retreating cliffs of the Suffolk coast, UK

    NASA Astrophysics Data System (ADS)

    Brooks, S. M.; Spencer, T.; Boreham, S.

    2012-06-01

    Understanding changing thresholds and mechanisms for retreat in soft rock cliffs is important under changing climates. This can be achieved through combining detailed field observation, long-term process and morphological monitoring and numerical modelling. The cliffs of the Suffolk coast, southern North Sea have exhibited long-term (1883-2010) recession rates of 3.5 m a- 1, rising to 4.7 m a- 1 in the period 1993-2010. Annual to biannual ground survey data, and the application of GIS techniques to digitised records of changing shoreline position from historic maps and aerial photography, reveal considerable decadal-scale variations in cliff recession, within which are nested inter-annual fluctuations in rates of retreat. Archival datasets on significant periods of onshore winds and their interaction with high water levels (including the incidence of storm surges) and rainstorm events are used to determine thresholds for cliff base erosion and its propagation upwards through the cliff profile. In addition, the ‘GEO-Slope' dynamic coupled hydrology-stability model is used to establish thresholds for cliff face failures driven by variations in rainfall inputs. Retreat mechanisms are complex, governed by cliff geology, both as a primary control on suction loss and through its interaction with basal marine conditions. The study allows a general model of cliff retreat for soft rock cliffs to be put forward, whereby a resistant basal platform is overlain by more erodible, weakly and moderately cemented sands and gravels. In this model, the varying balance between marine and terrestrial forcing factors are reflected in low (< 4 m a- 1), intermediate (4-7 m a- 1) and high (> 7 m a- 1) modes of cliff retreat.

  8. Multivariable Dynamic Ankle Mechanical Impedance With Active Muscles

    PubMed Central

    Lee, Hyunglae; Krebs, Hermano Igo; Hogan, Neville

    2015-01-01

    Multivariable dynamic ankle mechanical impedance in two coupled degrees-of-freedom (DOFs) was quantified when muscles were active. Measurements were performed at five different target activation levels of tibialis anterior and soleus, from 10% to 30% of maximum voluntary contraction (MVC) with increments of 5% MVC. Interestingly, several ankle behaviors characterized in our previous study of the relaxed ankle were observed with muscles active: ankle mechanical impedance in joint coordinates showed responses largely consistent with a second-order system consisting of inertia, viscosity, and stiffness; stiffness was greater in the sagittal plane than in the frontal plane at all activation conditions for all subjects; and the coupling between dorsiflexion–plantarflexion and inversion–eversion was small—the two DOF measurements were well explained by a strictly diagonal impedance matrix. In general, ankle stiffness increased linearly with muscle activation in all directions in the 2-D space formed by the sagittal and frontal planes, but more in the sagittal than in the frontal plane, resulting in an accentuated “peanut shape.” This characterization of young healthy subjects’ ankle mechanical impedance with active muscles will serve as a baseline to investigate pathophysiological ankle behaviors of biomechanically and/or neurologically impaired patients. PMID:25203497

  9. Predicting the Sources and Formation Mechanisms of Evolved Lunar Crust by Linking K/Ca Ratios of Lunar Granites to Analogous Terrestrial Igneous Rocks

    NASA Technical Reports Server (NTRS)

    Mills, R. D.; Simon, J. I.

    2012-01-01

    Although silicic rocks (i.e. granites and rhyolites) comprise a minor component of the sampled portion of the lunar crust, recent remote sensing studies [e.g., 1-4] indicate that several un-sampled regions of the Moon have significantly higher concentrations of silicic material (also high in [K], [U], and [Th]) than sampled regions. Within these areas are morphological features that are best explained by the existence of chemically evolved volcanic rocks. Observations of silicic domes [e.g., 1-5] suggest that sizable networks of silicic melt were present during crust formation. Isotopic data indicate that silicic melts were generated over a prolonged timespan from 4.3 to 3.9 Ga [e.g., 6-8]. The protracted age range and broad distribution of silicic rocks on the Moon indicate that their petrogenesis was an important mechanism for secondary crust formation. Understanding the origin and evolution of such silicic magmas is critical to determining the composition of the lunar crustal highlands and will help to distinguish between opposing ideas for the Moon's bulk composition and differentiation. The two main hypotheses for generating silicic melts on Earth are fractional crystallization or partial melting. On the Moon silicic melts are thought to have been generated during extreme fractional crystallization involving end-stage silicate liquid immiscibility (SLI) [e.g. 9, 10]. However, SLI cannot account for the production of significant volumes of silicic melt and its wide distribution, as reported by the remote global surveys [1, 2, 3]. In addition, experimental and natural products of SLI show that U and Th, which are abundant in the lunar granites and seen in the remote sensing data of the domes, are preferentially partitioned into the depolymerized ferrobasaltic magma and not the silicic portion [11, 12]. If SLI is not the mechanism that generated silicic magmas on the Moon then alternative processes such as fractional crystallization (only crystal

  10. Effect of Weathering Processes on Mineralogical and Mechanical Properties of Volcanic Rocks Used as Ballast Material for Railway Between Sabuncupinar and Kütahya in Western Turkey

    NASA Astrophysics Data System (ADS)

    Abiddin Erguler, Zeynal; Adıgüzel, Ömer; Derman, Mustafa

    2015-04-01

    Geomaterials used in engineering projects and man-made structures such as railway ballasts, buildings, historical structures, monuments and tombstones naturally weather as a result of various physico-chemical factors. Due to being long-term exposure to the anthroposphere, geomaterials used for these purposes provides important information to the researchers for understanding the effect of weathering processes on their time dependent physical, mineralogical and mechanical changes. Thus, researchers frequently can take advantage of available engineering time of man-made structures to assess weathering properties of the geomaterials used in their construction in terms of time dependent durability and stability of these structures. Considering the fact that railway ballasts produced from natural deposits of limestone, dolomite, granite, basalt etc., supply an important contribution for evaluation weathering processes, a research was carried out to determine the effect of weathering as a function of time on physical, mineralogical and mechanical properties of ballasts used for railway between Kütahya and Sabuncupınar in western Turkey. For this purpose, fresh and weathered rock samples exposed to physical and chemical weathering processes at different times were collected from quarry located in Sabuncupınar and nearby railway. This volcanic rock was previously classified as basalt based on the detailed mineralogical and geochemical analyses performed at the laboratories of the Mineral Research & Exploration General Directorate located in Ankara (Turkey). In-situ characteristics of sampling site were also investigated at different locations of quarry site by line surveying technique to describe the influence of discontinuity conditions on the weathering rate of selected rocks. Several techniques were utilized to determine time dependent deterioration in mineralogical and chemical composition of these samples for understanding their weathering rate. The porosity, water

  11. Drill-back studies examine fractured, heated rock

    SciTech Connect

    Wollenberg, H.A.; Flexser, S.; Myer, L.R.

    1990-01-01

    To investigate the effects of heating on the mineralogical, geochemical, and mechanical properties of rock by high-level radioactive waste, cores are being examined from holes penetrating locations where electric heaters simulated the presence of a waste canister, and from holes penetration natural hydrothermal systems. Results to date indicate the localized mobility and deposition of uranium in an open fracture in heated granitic rock, the mobility of U in a breccia zone in an active hydrothermal system in tuff, and the presence of U in relatively high concentration in fracture-lining material in tuff. Mechanical -- property studies indicate that differences in compressional- and shear-wave parameters between heated and less heated rock can be attributed to differences in the density of microcracks. Emphasis has shifted from initial studies of granitic rock at Stripa, Sweden to current investigations of welded tuff at the Nevada Test Site. 7 refs., 8 figs.

  12. Continental Basaltic Rocks

    NASA Astrophysics Data System (ADS)

    Farmer, G. L.

    2003-12-01

    During the past few decades, geochemical studies of continental basaltic rocks and their petrologic kin have become mainstays of studies of the continental lithosphere. These igneous rocks have taken on such an important role largely because the chemical and isotopic composition of continental basaltic rocks and their mantle (see Chapter 2.05) and crustal xenoliths (see Chapter 3.01) provide the best proxy record available to earth scientists for the chemical and physical evolution of the deep continental lithosphere and underlying mantle, areas that are otherwise resistant to direct study. Keeping this in mind, the primary goal of this chapter is to illustrate how geochemical data can be used both to assess the origin of these rocks and to study the evolution of the continental lithosphere.A complete overview of continental basaltic rocks will not be attempted here, because continental "basalts" come in too wide a range of compositions, and because of the sheer volume of geochemical data available for such rocks worldwide. The scope of the chapter is limited to a discussion of a select group of ultramafic to mafic composition "intraplate" continental igneous rocks consisting primarily of kimberlites, potassic and sodic alkali basalts, and continental flood basalts. Igneous rocks forming at active continental margins, such as convergent or transform plate margins, are important examples of continental magmatism but are not directly discussed here (convergent margin magmas are discussed in Chapters 2.11, 3.11, and 3.18). The geochemistry of intraplate igneous rocks of the ocean basins are covered in Chapters 2.04 and 3.16. Although basaltic magmatism has occurred throughout the Earths history, the majority of the examples presented here are from Mesozoic and Cenozoic volcanic fields due to the more complete preservation of younger continental mafic igneous rocks. While considerable effort has been expended in studying the chemical differentiation of mafic magmas

  13. Differential MSC activation leads to distinct mononuclear leukocyte binding mechanisms

    NASA Astrophysics Data System (ADS)

    Kota, Daniel J.; Dicarlo, Bryan; Hetz, Robert A.; Smith, Philippa; Cox, Charles S.; Olson, Scott D.

    2014-04-01

    Advances in the field of Multipotent Mesenchymal Stromal cell (MSC) biology have demonstrated that MSCs can improve disease outcome when `activated' to exert immunomodulatory effects. However, the precise mechanisms modulating MSC-immune cells interactions remain largely elusive. In here, we activated MSC based on a recent polarization paradigm, in which MSCs can be polarized towards a pro- or anti-inflammatory phenotype depending on the Toll-like receptor stimulated, to dissect the mechanisms through which MSCs physically interact with and modulate leukocytes in this context. Our data show that MSCs activated through the Toll-like receptor (TLR) 4 pathway increased VCAM-1 and ICAM-1 dependent binding of leukocytes. On the other hand, TLR3 stimulation strongly increases leukocytes affinity to MSC comparatively, through the formation of cable-like hyaluronic acid structures. In addition, TLR4 activation elicited secretion of pro-inflammatory mediators by MSCs, whereas TLR3-activated MSCs displayed a milder pro-inflammatory phenotype, similar to inactivated MSCs. However, the differently activated MSCs maintained their ability to suppress leukocyte activation at similar levels in our in vitro model, and this immunomodulatory property was shown here to be partially mediated by prostaglandin. These results reinforce the concept that alternate activation profiles control MSC responses and may impact the therapeutic use of MSCs.

  14. Mechanism of dual specificity kinase activity of DYRK1A.

    PubMed

    Walte, Agnes; Rüben, Katharina; Birner-Gruenberger, Ruth; Preisinger, Christian; Bamberg-Lemper, Simone; Hilz, Nikolaus; Bracher, Franz; Becker, Walter

    2013-09-01

    The function of many protein kinases is controlled by the phosphorylation of a critical tyrosine residue in the activation loop. Dual specificity tyrosine-phosphorylation-regulated kinases (DYRKs) autophosphorylate on this tyrosine residue but phosphorylate substrates on aliphatic amino acids. This study addresses the mechanism of dual specificity kinase activity in DYRK1A and related kinases. Tyrosine autophosphorylation of DYRK1A occurred rapidly during in vitro translation and did not depend on the non-catalytic domains or other proteins. Expression in bacteria as well as in mammalian cells revealed that tyrosine kinase activity of DYRK1A is not restricted to the co-translational autophosphorylation in the activation loop. Moreover, mature DYRK1A was still capable of tyrosine autophosphorylation. Point mutants of DYRK1A and DYRK2 lacking the activation loop tyrosine showed enhanced tyrosine kinase activity. A series of structurally diverse DYRK1A inhibitors was used to pharmacologically distinguish different conformational states of the catalytic domain that are hypothesized to account for the dual specificity kinase activity. All tested compounds inhibited substrate phosphorylation with higher potency than autophosphorylation but none of the tested inhibitors differentially inhibited threonine and tyrosine kinase activity. Finally, the related cyclin-dependent kinase-like kinases (CLKs), which lack the activation loop tyrosine, autophosphorylated on tyrosine both in vitro and in living cells. We propose a model of DYRK autoactivation in which tyrosine autophosphorylation in the activation loop stabilizes a conformation of the catalytic domain with enhanced serine/threonine kinase activity without disabling tyrosine phosphorylation. The mechanism of dual specificity kinase activity probably applies to related serine/threonine kinases that depend on tyrosine autophosphorylation for maturation. PMID:23809146

  15. UNDERSTANDING THE MECHANISMS OF CHANGE IN CHILDREN'S PHYSICAL ACTIVITY PROGRAMS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There is a lack of understanding of why childhood physical activity interventions succeed or fail. A model is proposed that relates program process to mediating variables and outcomes. Using the mdoel to design and evaluate interventions could result in a greater understanding of the mechanisms of c...

  16. Alkali-activated cementitious materials: Mechanisms, microstructure and properties

    NASA Astrophysics Data System (ADS)

    Jiang, Weimin

    The goal of this study was to examine the activation reaction, microstructure, properties, identify the mechanisms of activation, and achieve an enhanced understanding of activation processes occurring during the synthesis of alkali activated cementitious materials (AAC). The discussions classify the following categories. (1) alkali activated slag cement; (2) alkali activated portland-slag cement; (3) alkali activated fly ash-slag cement; (4) alkali activated pozzolana-lime cement; (5) alkali activated pozzolana cement. The activators involved are NaOH, KOH; Nasb2SOsb4;\\ Nasb2COsb3;\\ CaSOsb4, and soluble silicate of sodium and potassium. The effect of alkali activation on the microstructure of these materials were analyzed at the micro-nanometer scale by SEM, EDS, ESEM, and TEM. Also sp{29}Si and sp{27}Al MAS-NMR, IR, Raman, TGA, and DTA were performed to characterize the phase in these systems. Slag, fly ash, silica fume, as well as blended cements containing mixtures of these and other components were characterized. A set of ordinary portland cement paste samples served as a control. This study confirmed that AAC materials have great potential because they could generate very early high strength, greater durability and high performance. Among the benefits to be derived from this research is a better understanding of the factors that control concrete properties when using AAC materials, and by controlling the chemistry and processing to produce desired microstructures and properties, as well as their durability.

  17. Modeling study of the small-scale mantle convection in the subduction zone mantle wedge including the melting mechanism of mantle rocks

    NASA Astrophysics Data System (ADS)

    Yamamoto, M.; Tamura, Y.

    2014-12-01

    It is observed that subduction zone mantle wedge is not uniform even in the direction along the overlying island-arc that is perpendicular to the subducting direction. The hot fingers model is a hypothetical model specifying the three dimensional structural variation within the mangle wedge; it assumes that there is a fingers-like stripe pattern of mechanical and thermodynamical properties within the wedge. Those non-uniformity appears over the arc crust as nonuniform distribution of volcanic eruptions. Indeed, quaternary volcanoes in the NE Japan arc could be grouped into ten volcano clusters striking transverse to the arc. These have an average width of ∼50 km, and are separated by parallel gaps 30-75 km wide. Moreover, the structure of the mantle wedge and arc crust beneath the NE Japan arc and the Izu-Bonin-Mariana arc, respectively, suggest that the third dimension, lying along the strike of the arc, is necessary to understand the actual production of magmas in subduction zones. To explore the physical and mathematical mechanism of formation of the hot-fingers pattern, we develop a model of mantle convection in the mantle wedge. Our model incorporates the melting mechanism of the mantle rocks, which affect temperature and velocity of mantle. Our model produces a spatiotemporal pattern in those variables. The obtained results are compared with the spatiotemporal patterns observed in the NE Japan arc.

  18. Chemical and Microstructural Changes During Development of Mixed Ultramafic-quartzofeldspathic Fault Rock and the Effect on Mechanical Behaviour; Observations from SAFOD Gouge

    NASA Astrophysics Data System (ADS)

    Scott, J.; Toy, V. G.; Mitchell, T. M.; Goldsby, D. L.; Tullis, T. E.

    2011-12-01

    serpentine, albite grains, and a clay with a composition transitional between vermiculite and saponite. As there is no calcium reservoir within this rock, the Ca component to the clays must have been introduced; perhaps in fluids that deposited the carbonate while redistributing Al and Mg+Fe, respectively, from sedimentary and ultramafic clasts. We envisage that talc and clay were able to form during this fluid fluxing by exploiting the mesh-textured serpentine. The resulting aggregate was mechanically less resistant to deformation than intact serpentine and was therefore preferentially sheared into the gouge. Mixing of serpentine into the (primarily quartzo-feldspathic) gouge provided a source of Mg, which contributed to the formation of vermiculite-saponite-type clays. These observations illustrate 1) the importance of changing modal composition and phase arrangement on mechanical strength of a fault rock aggregate, and 2) that the compositional/mineralogical changes important in fault zone weakening can be very effective if mechanical mixing and small scale fluid-assisted dissolution-precipitation can occur together.

  19. Nox family NADPH oxidases: Molecular mechanisms of activation.

    PubMed

    Brandes, Ralf P; Weissmann, Norbert; Schröder, Katrin

    2014-11-01

    NADPH oxidases of the Nox family are important enzymatic sources of reactive oxygen species (ROS). Numerous homologue-specific mechanisms control the activity of this enzyme family involving calcium, free fatty acids, protein-protein interactions, intracellular trafficking, and posttranslational modifications such as phosphorylation, acetylation, or sumoylation. After a brief review on the classic pathways of Nox activation, this article will focus on novel mechanisms of homologue-specific activity control and on cell-specific aspects which govern Nox activity. From these findings of the recent years it must be concluded that the activity control of Nox enzymes is much more complex than anticipated. Moreover, depending on the cellular activity state, Nox enzymes are selectively activated or inactivated. The complex upstream signaling aspects of these events make the development of "intelligent" Nox inhibitors plausible, which selectively attenuate disease-related Nox-mediated ROS formation without altering physiological signaling ROS. This approach might be of relevance for Nox-mediated tissue injury in ischemia-reperfusion and inflammation and also for chronic Nox overactivation as present in cancer initiation and cardiovascular disease.

  20. Active mechanics and geometry of adherent cells and cell colonies

    NASA Astrophysics Data System (ADS)

    Banerjee, Shiladitya

    2014-03-01

    Measurements of traction stresses exerted by adherent cells or cell colonies on elastic substrates have yielded new insight on how the mechanical and geometrical properties of the substrate regulate cellular force distribution, mechanical energy, spreading, morphology or stress ber architecture. We have developed a generic mechanical model of adherent cells as an active contractile gel mechanically coupled to an elastic substrate and to neighboring cells in a tissue. The contractile gel model accurately predicts the distribution of cellular and traction stresses as observed in single cell experiments, and captures the dependence of cell shape, traction stresses and stress ber polarization on the substrate's mechanical and geometrical properties. The model further predicts that the total strain energy of an adherent cell is solely regulated by its spread area, in agreement with recent experiments on micropatterned substrates with controlled geometry. When used to describe the behavior of colonies of adherent epithelial cells, the model demonstrates the crucial role of the mechanical cross-talk between intercellular and extracellular adhesion in regulating traction force distribution. Strong intercellular mechanical coupling organizes traction forces to the colony periphery, whereas weaker intercellular coupling leads to the build up of traction stresses at intercellular junctions. Furthermore, in agreement with experiments on large cohesive keratinocyte colonies, the model predicts a linear scaling of traction forces with the colony size. This scaling suggests the emergence of an effective surface tension as a scale-free material property of the adherent tissue, originating from actomyosin contractility.

  1. Mechanisms of active control in cylindrical fuselage structures

    NASA Technical Reports Server (NTRS)

    Silcox, R. J.; Lester, H. C.; Fuller, C. R.

    1987-01-01

    This paper summarizes ongoing efforts to understand and exploit active control techniques for low frequency noise suppression in aerospace applications. Analytical models are utilized in an effort to understand the mechanisms that govern noise transmission into acoustic spaces enclosed by lightweight structures and to examine the results of experimental implementations of active control schemes. Emphasis is placed on attaining global noise reductions using a minimum number of actuators rather than localized control over many subregions. This program has demonstrated the effect of synchrophasing and interface modal filtering, in limiting the modal density within the acoustic space, and how strong reactive effects may occur in two dimensional geometries. Finally, the performance of active control systems utilizing acoustic and vibration actuators is evaluated. Suppressions of 10 to 30 dB are demonstrated in practice, and performance is discussed in relation to the physical mechanisms and parameters of the system.

  2. Risk Analysis and Prediction of Floor Failure Mechanisms at Longwall Face in Parvadeh-I Coal Mine using Rock Engineering System (RES)

    NASA Astrophysics Data System (ADS)

    Aghababaei, Sajjad; Saeedi, Gholamreza; Jalalifar, Hossein

    2016-05-01

    The floor failure at longwall face decreases productivity and safety, increases operation costs, and causes other serious problems. In Parvadeh-I coal mine, the timber is used to prevent the puncture of powered support base into the floor. In this paper, a rock engineering system (RES)-based model is presented to evaluate the risk of floor failure mechanisms at the longwall face of E 2 and W 1 panels. The presented model is used to determine the most probable floor failure mechanism, effective factors, damaged regions and remedial actions. From the analyzed results, it is found that soft floor failure is dominant in the floor failure mechanism at Parvadeh-I coal mine. The average of vulnerability index (VI) for soft, buckling and compressive floor failure mechanisms was estimated equal to 52, 43 and 30 for both panels, respectively. By determining the critical VI for soft floor failure mechanism equal to 54, the percentage of regions with VIs beyond the critical VI in E 2 and W 1 panels is equal to 65.5 and 30, respectively. The percentage of damaged regions showed that the excess amount of used timber to prevent the puncture of weak floor below the powered support base is equal to 4,180,739 kg. RES outputs and analyzed results showed that setting and yielding load of powered supports, length of face, existent water at face, geometry of powered supports, changing the cutting pattern at longwall face and limiting the panels to damaged regions with supercritical VIs could be considered to control the soft floor failure in this mine. The results of this research could be used as a useful tool to identify the damaged regions prior to mining operation at longwall panel for the same conditions.

  3. Insights into the Thiamine Diphosphate Enzyme Activation Mechanism: Computational Model for Transketolase Using a Quantum Mechanical/Molecular Mechanical Method.

    PubMed

    Nauton, Lionel; Hélaine, Virgil; Théry, Vincent; Hecquet, Laurence

    2016-04-12

    We propose the first computational model for transketolase (TK), a thiamine diphosphate (ThDP)-dependent enzyme, using a quantum mechanical/molecular mechanical method on the basis of crystallographic TK structures from yeast and Escherichia coli, together with experimental kinetic data reported in the literature with wild-type and mutant TK. This model allowed us to define a new route for ThDP activation in the enzyme environment. We evidenced a strong interaction between ThDP and Glu418B of the TK active site, itself stabilized by Glu162A. The crucial point highlighted here is that deprotonation of ThDP C2 is not performed by ThDP N4' as reported in the literature, but by His481B, involving a HOH688A molecule bridge. Thus, ThDP N4' is converted from an amino form to an iminium form, ensuring the stabilization of the C2 carbanion or carbene. Finally, ThDP activation proceeds via an intermolecular process and not by an intramolecular one as reported in the literature. More generally, this proposed ThDP activation mechanism can be applied to some other ThDP-dependent enzymes and used to define the entire TK mechanism with donor and acceptor substrates more accurately.

  4. Structure and Mechanism of the Phosphotyrosyl Phosphatase Activator

    SciTech Connect

    Chao,Y.; Xing, Y.; Chen, Y.; Xu, Y.; Lin, Z.; Li, Z.; Jeffrey, P.; Stock, J.; Shi, Y.

    2006-01-01

    Phosphotyrosyl phosphatase activator (PTPA), also known as PP2A phosphatase activator, is a conserved protein from yeast to human. Here we report the 1.9 {angstrom} crystal structure of human PTPA, which reveals a previously unreported fold consisting of three subdomains: core, lid, and linker. Structural analysis uncovers a highly conserved surface patch, which borders the three subdomains, and an associated deep pocket located between the core and the linker subdomains. The conserved surface patch and the deep pocket are responsible for binding to PP2A and ATP, respectively. PTPA and PP2A A-C dimer together constitute a composite ATPase. PTPA binding to PP2A results in a dramatic alteration of substrate specificity, with enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity. This function of PTPA strictly depends on the composite ATPase activity. These observations reveal significant insights into the function and mechanism of PTPA and have important ramifications for understanding PP2A function.

  5. The character and evolution of fault rocks from the Phase 3 SAFOD core and potential weakening mechanisms along the San Andreas Fault

    NASA Astrophysics Data System (ADS)

    Holdsworth, Robert; van Diggelen, E. W. E.; Spiers, C. J.; de Bresser, H.; Smith, S. A. F.; Bowen, L.

    2010-05-01

    In the region of the SAFOD borehole, the San Andreas Fault (SAF) separates two very different geological terranes referred to here as the Salinian and Great Valley blocks (SB, SVB). Whilst material was not collected from the SB-GVB terrane boundary, the cores preserve a diverse range of fault rocks. Not all of these necessarily formed at the same depth, although the amount of exhumation is likely fairly limited. The distribution of deformation is asymmetric, with a broad (200m wide) intensely deformed region developed in the GVB located NE of the terrane boundary; this includes two narrow zones of active creep that have deformed the borehole casing. Microstructurally, low strain domains (most of Core 1, significant parts of Core 3) preserve clear protolith structures, with highly localised evidence for classic upper crustal cataclastic brittle faulting processes and associated fluid flow. The GVB in particular shows clear geological evidence for both fluid pressure and differential stress cycling (variable modes of hydrofacture associated with faults) during seismicity. There is also evidence in all minor faults for the operation of solution-precipitation creep. High strain domains (much of Core 2, parts of Core 3) are characterised by the development of foliated cataclasites and gouge, with variable new growth of fine-grained, interconnected phyllosilicate networks (predominantly smectite-bearing mixed layer clays). Many of the gouges are characterised by the development of S-C fabrics and asymmetric folds. Reworking and reactivation is widespread manifested by: i) the preservation of one or more earlier generations of gouge preserved as clasts; and ii) by the development of later interconnected, polished and striated slip surfaces at low angles or sub-parallel to the foliation. These are coated with thin smectitic phyllosilicate films and are closely associated with the development of lozenge, arrow-head and triangular mineral veins (mostly calcite) precipitated

  6. Lung epithelial cell-derived extracellular vesicles activate macrophage-mediated inflammatory responses via ROCK1 pathway.

    PubMed

    Moon, H-G; Cao, Y; Yang, J; Lee, J H; Choi, H S; Jin, Y

    2015-12-10

    Despite decades of research, the pathogenesis of acute respiratory distress syndrome (ARDS) remains poorly understood, thus impeding the development of effective treatment. Diffuse alveolar damage (DAD) and lung epithelial cell death are prominent features of ARDS. Lung epithelial cells are the first line of defense after inhaled stimuli, such as in the case of hyperoxia. We hypothesized that lung epithelial cells release 'messenger' or signaling molecules to adjacent or distant macrophages, thereby initiating or propagating inflammatory responses after noxious insult. We found that, after hyperoxia, a large amount of extracellular vesicles (EVs) were generated and released into bronchoalveolar lavage fluid (BALF). These hyperoxia-induced EVs were mainly derived from live lung epithelial cells as the result of hyperoxia-associated endoplasmic reticulum (ER) stress. These EVs were remarkably different from epithelial 'apoptotic bodies', as reflected by the significantly smaller size and differentially expressed protein markers. These EVs fall mainly in the size range of the exosomes and smaller microvesicles (MVs) (50-120 nm). The commonly featured protein markers of apoptotic bodies were not found in these EVs. Treating alveolar macrophages with hyperoxia-induced, epithelial cell-derived EVs led to an increased secretion of pro-inflammatory cytokines and macrophage inflammatory protein 2 (MIP-2). Robustly increased macrophage and neutrophil influx was found in the lung tissue of the mice intranasally treated with hyperoxia-induced EVs. It was determined that EV-encapsulated caspase-3 was largely responsible for the alveolar macrophage activation via the ROCK1 pathway. Caspase-3-deficient EVs induced less cytokine/MIP-2 release, reduced cell counts in BALF, less neutrophil infiltration and less inflammation in lung parenchyma, both in vitro and in vivo. Furthermore, the serum circulating EVs were increased and mainly derived from lung epithelial cells after

  7. A 10,300-year-old permafrost core from the active rock glacier Lazaun, southern Ötztal Alps (South Tyrol, northern Italy)

    NASA Astrophysics Data System (ADS)

    Krainer, Karl; Bressan, David; Dietre, Benjamin; Haas, Jean Nicolas; Hajdas, Irka; Lang, Kathrin; Mair, Volkmar; Nickus, Ulrike; Reidl, Daniel; Thies, Hansjörg; Tonidandel, David

    2015-03-01

    Two cores were drilled on rock glacier Lazaun in the southern Ötztal Alps (N Italy). The average ice content of core Lazaun I is 43 vol.% and of core Lazaun II is 22 vol.%. Radiocarbon dating of plant macrofossil remains of core Lazaun I yielded ages ranging from 8960 cal yr BP at a depth of ca. 23.5 m to 2240 cal yr BP at a depth of 2.8 m, indicating that the ice near the base is approximately 10,300 yr old. The rock glacier was intact since that time and the ice persisted even during warm periods of the Holocene. An ice-free debris layer between 16.8 and 14.7 m separates the rock glacier into two frozen bodies. Inclinometer measurements indicate that both frozen bodies are active and that deformation occurs within a shear horizon at a depth of 20-25 m at the base of the lower frozen body and to a minor extent at a depth of approximately 14 m at the base of the upper frozen body. The ice-free debris layer in the middle of the Lazaun rock glacier indicates a more than five centennial long drought period, which dates to about 4300-3740 cal yr BP.

  8. Novel mechanisms for activated protein C cytoprotective activities involving noncanonical activation of protease-activated receptor 3.

    PubMed

    Burnier, Laurent; Mosnier, Laurent O

    2013-08-01

    The direct cytoprotective activities of activated protein C (APC) on cells convey therapeutic, relevant, beneficial effects in injury and disease models in vivo and require the endothelial protein C receptor (EPCR) and protease activated receptor 1 (PAR1). Thrombin also activates PAR1, but its effects on cells contrast APC's cytoprotective effects. To gain insights into mechanisms for these contrasting cellular effects, protease activated receptor 3 (PAR3) activation by APC and thrombin was studied. APC cleaved PAR3 on transfected and endothelial cells in the presence of EPCR. Remarkably, APC cleaved a synthetic PAR3 N-terminal peptide at Arg41, whereas thrombin cleaved at Lys38. On cells, APC failed to cleave R41Q-PAR3, whereas K38Q-PAR3 was still cleaved by APC but not by thrombin. PAR3 tethered-ligand peptides beginning at amino acid 42, but not those beginning at amino acid 39, conveyed endothelial barrier-protective effects. In vivo, the APC-derived PAR3 tethered-ligand peptide, but not the thrombin-derived PAR3 peptide, blunted vascular endothelial growth factor (VEGF)-induced vascular permeability. These data indicate that PAR3 cleavage by APC at Arg41 can initiate distinctive APC-like cytoprotective effects. These novel insights help explain the differentiation of APC's cytoprotective versus thrombin's proinflammatory effects on cells and suggest a unique contributory role for PAR3 in the complex mechanisms underlying APC cytoprotective effects. PMID:23788139

  9. Class I Microcins: Their Structures, Activities, and Mechanisms of Resistance

    NASA Astrophysics Data System (ADS)

    Severinov, Konstantin; Semenova, Ekaterina; Kazakov, Teymur

    Microcin J25, microcin B17, and microcin C7-C51 are the three known members of class I posttranslationally modified microcins (heavily posttranslationally modified antibacterial peptides produced by Enterobacteriaceae with molecular weights of less than 5 kDa). The three microcins are unrelated to each other; they have structures that are highly atypical for ribosomally synthesized peptides and target essential molecular machines that are validated drug targets. In this chapter, available data on mechanisms of action, structure-activity relationships, and immunity mechanisms for class I microcins and related compounds are discussed.

  10. Application of epithermal neutron activation in multielement analysis of silicate rocks employing both coaxial Ge(Li) and low energy photon detector systems

    USGS Publications Warehouse

    Baedecker, P.A.; Rowe, J.J.; Steinnes, E.

    1977-01-01

    The instrumental activation analysis of silicate rocks using epithermal neutrons has been studied using both high resolution coaxial Ge(Li) detectors and low energy photon detectors, and applied to the determination of 23 elements in eight new U.S.G.S. standard rocks. The analytical use X-ray peaks associated with electron capture or internal conversion processes has been evaluated. Of 28 elements which can be considered to be determinable by instrumental means, the epithermal activation approach is capable of giving improved sensitivity and precision in 16 cases, over the normal INAA procedure. In eleven cases the use of the low energy photon detector is thought to show advantages over convertional coaxial Ge(Li) spectroscopy. ?? 1977 Akade??miai Kiado??.

  11. Multivariable Static Ankle Mechanical Impedance With Active Muscles.

    PubMed

    Lee, Hyunglae; Ho, Patrick; Rastgaar, Mohammad; Krebs, Hermano Igo; Hogan, Neville

    2014-01-01

    This paper reports quantification of multivariable static ankle mechanical impedance when muscles were active. Repetitive measurements using a highly backdrivable therapeutic robot combined with robust function approximation methods enabled reliable characterization of the nonlinear torque-angle relation at the ankle in two coupled degrees of freedom simultaneously, a combination of dorsiflexion-plantarflexion and inversion-eversion, and how it varied with muscle activation. Measurements on 10 young healthy seated subjects quantified the behavior of the human ankle when muscles were active at 10% of maximum voluntary contraction. Stiffness, a linear approximation to static ankle mechanical impedance, was estimated from the continuous vector field. As with previous measurements when muscles were maximally relaxed, we found that ankle stiffness was highly direction-dependent, being weakest in inversion/eversion. Predominantly activating a single muscle or co-contracting antagonistic muscles significantly increased ankle stiffness in all directions but it increased more in the sagittal plane than in the frontal plane, accentuating the relative weakness of the ankle in the inversion-eversion direction. Remarkably, the observed increase was not consistent with simple superposition of muscle-generated stiffness, which may be due to the contribution of unmonitored deep ankle muscles. Implications for the assessment of neuro-mechanical disorders are discussed.

  12. Modeling Transport of Viruses in Fractured Rock

    NASA Astrophysics Data System (ADS)

    Sleep, B. E.; Mondal, P. K.

    2011-12-01

    Fractured rock aquifers are frequently used for water supply for human consumption. In many instances the fractured rock aquifers are vulnerable to contamination by pathogens, including viruses, due to co-location of on-site septic systems, wastewater discharges, biosolids and agricultural activities. Approximately half of the illnesses associated with groundwater consumption in the Unites States have been attributed to viral contamination. A number of these cases have been related to transport of viruses from septic systems to drinking water wells. Despite the potential for rapid transport of viruses through rock fractures to drinking water wells, the understanding of virus transport in fractured rock is limited. In particular, the impacts of virus size, fracture aperture variability and roughness, matrix porosity, groundwater velocity, and geochemical conditions have not been well studied. In this study, a multidimensional model for virus transport in variable aperture fractures is presented. The model is applied to laboratory experiments on transport of virus-sized latex microspheres (0.02 and 0.2 microns) and bacteriophages (MS2 and PR772) in artificially fractured dolomite rocks. In these experiments significant impacts of particle size, fracture characteristics, groundwater velocity, and geochemistry were observed. Given the variability in aperture distribution and associated spatial variation in groundwater flow field, one-dimensional models were not suitable for a comprehensive evaluation of the mechanisms governing the microsphere and bacteriophage transport. Various relationships for virus retention (attachment and detachment) are evaluated to provide insight into the governing processes in virus transport in fractured rock. In addition, the role of virus size, fracture aperture variability, fracture roughness, fracture surface charge, matrix porosity, groundwater velocity, and ionic strength in virus transport are evaluated. Scale-up to the field is

  13. The microstructural character and evolution of fault rocks from the SAFOD core and potential weakening mechanisms along the San Andreas Fault (Invited)

    NASA Astrophysics Data System (ADS)

    Holdsworth, R. E.; van Diggelen, E.; Spiers, C.; de Bresser, J. H.; Smith, S. A.

    2009-12-01

    In the region of the SAFOD borehole, the San Andreas Fault (SAF) separates two very different geological terranes referred to here as the Salinian and Great Valley blocks (SB, GVB). The three sections of core preserve a diverse range of fault rocks and pass through the two currently active, highly localised slipping sections, the so-called ‘10480’ and ‘10830’ fault zones . These coincide with a broader region - perhaps as much as 100m wide - of high strain fault rocks formed at some time in the geological past, but now currently inactive. Both the slipping segments and older high strain zone(s) are developed in the GVB located NE of the terrane boundary. This is likely influenced by the phyllosilicate-rich protolith of the GVB and the large volume of trapped fluid known to exist NE and below the SAF in this region. Microstructurally, lower strain domains (most of Core 1 cutting the SB, significant parts of Core 3 cutting the GVB) preserve clear evidence for classic upper crustal cataclastic brittle faulting processes and associated fluid flow. The GVB in particular shows clear geological evidence for both fluid pressure and differential stress cycling (variable modes of hydrofacture associated with faults) during seismicity. There is also some evidence in all minor faults for the operation of limited amounts of solution-precipitation creep. High strain domains (much of Core 2 cutting the GVB, parts of Core 3 adjacent to the 10830 fault) are characterised by the development of foliated cataclasites and gouge largely due to the new growth of fine-grained phyllosilicate networks (predominantly smectite-bearing mixed layer clays, locally serpentinite, but not talc). The most deformed sections are characterised by the development of shear band fabrics and asymmetric folds. Reworking and reactivation is widespread manifested by: i) the preservation of one or more earlier generations of gouge preserved as clasts; and ii) by the development of later interconnected

  14. Forcing Open TRP channels: mechanical gating as a unifying activation mechanism

    PubMed Central

    Liu, Chao; Montell, Craig

    2015-01-01

    Transient receptor potential (TRP) proteins are cation channels that comprise a superfamily of molecular sensors that enable animals to detect a wide variety of environmental stimuli. This versatility enables vertebrate and invertebrate TRP channels to function in a diversity of senses, ranging from vision to taste, smell, touch, hearing, proprioception and thermosensation. Moreover, many individual TRP channels are activated through a surprising range of sensory stimuli. The multitasking nature of TRP channels raises the question as to whether seemingly disparate activators gate TRPs through common strategies. In this regard, a recent major advance is the discovery that a phospholipase C (PLC)-