Quantitative Classification of Rice (Oryza sativa L.) Root Length and Diameter Using Image Analysis.

PubMed

Gu, Dongxiang; Zhen, Fengxian; Hannaway, David B; Zhu, Yan; Liu, Leilei; Cao, Weixing; Tang, Liang

2017-01-01

Quantitative study of root morphological characteristics of plants is helpful for understanding the relationships between their morphology and function. However, few studies and little detailed and accurate information of root characteristics were reported in fine-rooted plants like rice (Oryza sativa L.). The aims of this study were to quantitatively classify fine lateral roots (FLRs), thick lateral roots (TLRs), and nodal roots (NRs) and analyze their dynamics of mean diameter (MD), lengths and surface area percentage with growth stages in rice plant. Pot experiments were carried out during three years with three rice cultivars, three nitrogen (N) rates and three water regimes. In cultivar experiment, among the three cultivars, root length of 'Yangdao 6' was longest, while the MD of its FLR was the smallest, and the mean diameters for TLR and NR were the largest, the surface area percentage (SAP) of TLRs (SAPT) was the highest, indicating that Yangdao 6 has better nitrogen and water uptake ability. High N rate increased the length of different types of roots and increased the MD of lateral roots, decreased the SAP of FLRs (SAPF) and TLRs, but increased the SAP of NRs (SAPN). Moderate decrease of water supply increased root length and diameter, water stress increased the SAPF and SAPT, but decreased SAPN. The quantitative results indicate that rice plant tends to increase lateral roots to get more surface area for nitrogen and water uptake when available assimilates are limiting under nitrogen and water stress environments.

Quantitative Classification of Rice (Oryza sativa L.) Root Length and Diameter Using Image Analysis

PubMed Central

Gu, Dongxiang; Zhen, Fengxian; Hannaway, David B.; Zhu, Yan; Liu, Leilei; Cao, Weixing; Tang, Liang

2017-01-01

Quantitative study of root morphological characteristics of plants is helpful for understanding the relationships between their morphology and function. However, few studies and little detailed and accurate information of root characteristics were reported in fine-rooted plants like rice (Oryza sativa L.). The aims of this study were to quantitatively classify fine lateral roots (FLRs), thick lateral roots (TLRs), and nodal roots (NRs) and analyze their dynamics of mean diameter (MD), lengths and surface area percentage with growth stages in rice plant. Pot experiments were carried out during three years with three rice cultivars, three nitrogen (N) rates and three water regimes. In cultivar experiment, among the three cultivars, root length of ‘Yangdao 6’ was longest, while the MD of its FLR was the smallest, and the mean diameters for TLR and NR were the largest, the surface area percentage (SAP) of TLRs (SAPT) was the highest, indicating that Yangdao 6 has better nitrogen and water uptake ability. High N rate increased the length of different types of roots and increased the MD of lateral roots, decreased the SAP of FLRs (SAPF) and TLRs, but increased the SAP of NRs (SAPN). Moderate decrease of water supply increased root length and diameter, water stress increased the SAPF and SAPT, but decreased SAPN. The quantitative results indicate that rice plant tends to increase lateral roots to get more surface area for nitrogen and water uptake when available assimilates are limiting under nitrogen and water stress environments. PMID:28103264

Micro-computed tomography evaluation of the preparation of long oval root canals in mandibular molars with the self-adjusting file.

PubMed

Paqué, Frank; Peters, Ove A

2011-04-01

The aim of this study was to assess the shaping potential of a novel nickel-titanium instrument, the self-adjusting file (SAF), in long oval root canals in distal roots in mandibular molars. Twenty mandibular molars with long oval distal root canals were selected and scanned preoperatively and postoperatively by using micro-computed tomography at an original resolution of 20 μm. Canals were shaped with the SAF, three-dimensionally reconstructed, and evaluated for volume, surface area, canal transportation, and prepared surface. Data were statistically contrasted by using paired t tests and regression analysis. Preoperatively, canal volume was 7.73 ± 2.13 mm(3), and canal area was 42.83 ± 8.14 mm(2). Volumes and surface areas increased significantly (P < .001) by 4.84 ± 1.73 mm(3) and 3.34 ± 1.73 mm(2), respectively, and no gross preparation errors were detected. Unprepared canal surface varied between individual canals, and mean unprepared surface was 23.5% ± 8.9%. Prepared areas were significantly larger compared with rotary canal preparation done in a previous study. Canal transportation scores were higher in the coronal root canal third (106 ± 50 μm) compared with the apical third (81 ± 49 μm). In vitro, preparation of long oval-shaped root canals in mandibular molars with the SAF was effective and safe. Moreover, shapes generated with the SAF were more complete compared with rotary canal preparation. Copyright © 2011 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Effect of finishing instrumentation using NiTi hand files on volume, surface area and uninstrumented surfaces in C-shaped root canal systems.

PubMed

Amoroso-Silva, P; Alcalde, M P; Hungaro Duarte, M A; De-Deus, G; Ordinola-Zapata, R; Freire, L G; Cavenago, B C; De Moraes, I G

2017-06-01

To assess the effect of 90°-oscillatory instrumentation with hand files on several morphological parameters (volume, surface area and uninstrumented surface) in C-shaped root canals after instrumentation using a single-file reciprocation system (Reciproc; VDW, Munich, Germany) and a Self-Adjusting File System (SAF; ReDent Nova, Ra'anana, Israel). Twenty mandibular second molars with C-shaped canals and C1 canal configurations were divided into two groups (n = 10) and instrumented with Reciproc and SAF instruments. A size 30 NiTi hand K-file attached to a 90°-oscillatory motion handpiece was used as final instrumentation in both groups. The specimens were scanned using micro-computed tomography after all procedures. Volume, surface area increase and uninstrumented root canal surface were analysed using CTAn software (Bruker-microCT, Kontich, Belgium). Also, the uninstrumented root canal surface was calculated for each canal third. All values were compared between groups using the Mann-Whitney test and within groups using the Wilcoxon's signed-rank test. Instrumentation with Reciproc significantly increased canal volume compared with instrumentation with SAF. Additionally, the canal volumes were significantly increased after 90°-oscillatory instrumentation (between and within group comparison; (P < 0.05)). Regarding the increase in surface area after all instrumentation protocols, statistical analysis only revealed significant differences in the within groups comparison (P < 0.05). Reciproc and SAF instrumentation yielded an uninstrumented root canal surface of 28% and 34%, respectively, which was not significantly different (P > 0.05). Final oscillatory instrumentation significantly reduced the uninstrumented root canal surface from 28% to 9% (Reciproc) and from 34% to 15% (SAF; P < 0.05). The apical and middle thirds exhibited larger uninstrumented root canal surfaces after the first instrumentation that was significantly reduced after oscillatory instrumentation (P < 0.05). The Reciproc and SAF system were associated with similar morphological parameters after instrumentation of mandibular second molars with C-shaped canals except for a higher canal volume increase in the Reciproc group compared to the SAF. Furthermore, the final use of 90°-oscillatory instrumentation using NiTi hand files significantly decreased the uninstrumented canal walls that remained after Reciproc and SAF instrumentation. © 2016 International Endodontic Journal. Published by John Wiley & Sons Ltd.

Mandibular molar root morphology in Neanderthals and Late Pleistocene and recent Homo sapiens.

PubMed

Kupczik, Kornelius; Hublin, Jean-Jacques

2010-11-01

Neanderthals have a distinctive suite of dental features, including large anterior crown and root dimensions and molars with enlarged pulp cavities. Yet, there is little known about variation in molar root morphology in Neanderthals and other recent and fossil members of Homo. Here, we provide the first comprehensive metric analysis of permanent mandibular molar root morphology in Middle and Late Pleistocene Homo neanderthalensis, and Late Pleistocene (Aterian) and recent Homo sapiens. We specifically address the question of whether root form can be used to distinguish between these groups and assess whether any variation in root form can be related to differences in tooth function. We apply a microtomographic imaging approach to visualise and quantify the external and internal dental morphologies of both isolated molars and molars embedded in the mandible (n=127). Univariate and multivariate analyses reveal both similarities (root length and pulp volume) and differences (occurrence of pyramidal roots and dental tissue volume proportion) in molar root morphology among penecontemporaneous Neanderthals and Aterian H. sapiens. In contrast, the molars of recent H. sapiens are markedly smaller than both Pleistocene H. sapiens and Neanderthals, but share with the former the dentine volume reduction and a smaller root-to-crown volume compared with Neanderthals. Furthermore, we found the first molar to have the largest average root surface area in recent H. sapiens and Neanderthals, although in the latter the difference between M(1) and M(2) is small. In contrast, Aterian H. sapiens root surface areas peak at M(2). Since root surface area is linked to masticatory function, this suggests a distinct occlusal loading regime in Neanderthals compared with both recent and Pleistocene H. sapiens. Copyright © 2010 Elsevier Ltd. All rights reserved.

Physical properties of root cementum: Part 18. The extent of root resorption after the application of light and heavy controlled rotational orthodontic forces for 4 weeks: a microcomputed tomography study.

PubMed

Wu, Andy T J; Turk, Tamer; Colak, Canan; Elekdağ-Turk, Selma; Jones, Allan S; Petocz, Peter; Darendeliler, M Ali

2011-05-01

The aim of this prospective randomized clinical trial was to quantitatively measure and compare the locations, dimensions, and volume of root resorption craters in human premolars after the application of controlled light and heavy rotational orthodontic forces over a 28-day (4-week) period. Fifteen patients requiring bilateral extraction of maxillary first premolars as part of their orthodontic treatment were recruited for this study. Each patient received a heavy (225 g) rotational force on 1 premolar and a light (25 g) rotational force on the contralateral premolar. Orthodontic rotational forces were applied over 28 days with buccal and palatal cantilever springs; 0.016-inch beta-titanium molybdenum alloys were used to apply the light force and 0.018-inch stainless steel was used for the heavy force. After the 28-day experimental period, the upper first premolars were extracted under stringent protocols to prevent root surface damage. The samples were then scanned using a microcomputed tomography (micro-CT) scan x-ray system (SkyScan 1072, Skyscan, Aartselaar, Belgium), and analyzed using convex hull algorithm (CHULL2D; University of Sydney, Sydney, Australia) software to obtain direct volumetric measurements. The mean volume of resorption craters was 0.42 in the light force group and 0.51 in the heavy force group (P = 0.013). When separated at the root level, the difference in volume of root resorption craters between the 2 groups was significantly different only at the midlevel (P = 0.001). Root resorption craters were consistently detected at the boundaries between the buccal and distal surfaces and the mesial and lingual surfaces. The result supports our hypothesis that positive areas develop significantly more root resorption craters at all 3 levels, as compared with minimal areas (paired t test <0.001). Heavy rotational forces caused more root resorption than light rotational forces and compression areas (buccal-distal and lingual-mesial surfaces in this study) showed significantly higher root resorption than other areas at all levels of the root. Copyright © 2011 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved.

Extracting Metrics for Three-dimensional Root Systems: Volume and Surface Analysis from In-soil X-ray Computed Tomography Data.

PubMed

Suresh, Niraj; Stephens, Sean A; Adams, Lexor; Beck, Anthon N; McKinney, Adriana L; Varga, Tamas

2016-04-26

Plant roots play a critical role in plant-soil-microbe interactions that occur in the rhizosphere, as well as processes with important implications to climate change and crop management. Quantitative size information on roots in their native environment is invaluable for studying root growth and environmental processes involving plants. X-ray computed tomography (XCT) has been demonstrated to be an effective tool for in situ root scanning and analysis. We aimed to develop a costless and efficient tool that approximates the surface and volume of the root regardless of its shape from three-dimensional (3D) tomography data. The root structure of a Prairie dropseed (Sporobolus heterolepis) specimen was imaged using XCT. The root was reconstructed, and the primary root structure was extracted from the data using a combination of licensed and open-source software. An isosurface polygonal mesh was then created for ease of analysis. We have developed the standalone application imeshJ, generated in MATLAB(1), to calculate root volume and surface area from the mesh. The outputs of imeshJ are surface area (in mm(2)) and the volume (in mm(3)). The process, utilizing a unique combination of tools from imaging to quantitative root analysis, is described. A combination of XCT and open-source software proved to be a powerful combination to noninvasively image plant root samples, segment root data, and extract quantitative information from the 3D data. This methodology of processing 3D data should be applicable to other material/sample systems where there is connectivity between components of similar X-ray attenuation and difficulties arise with segmentation.

[Phytoremediation of Petroleum Contaminated Soils with Iris pseudacorus L. and the Metabolic Analysis in Roots].

PubMed

Wang, Ya-nan; Cheng, Li-juan; Zhou, Qi-xing

2016-04-15

In this study, we performed a greenhouse pot-culture experiment to investigate the potential of a wild ornamental plant Iris pseudacorus L. in remediating petroleum contaminated soils from the Dagang Oilfield in Tianjin, China. The results suggested that Iris pseudacorus L. had great resistance to ≤ 40,000 mg · kg(⁻¹ of total petroleum hydrocarbons (TPHs). The removal rate of TPHs with concentrations of 10,000 mg · kg⁻¹, 20,000 mg · kg⁻¹ and 40,000 mg · kg⁻¹ in soils by Iris pseudacorus L. was 42.1%, 33.1% 31.2%, respectively, much higher than those in the corresponding controls (31.8%, 21.3% 11.9%, respectively) (P < 0.05). The root specific surface area of Iris pseudacorus L. was determined by the root scanner. The results suggested that TPHs with concentrations of 10,000 mg · kg⁻¹, 20,000 mg · kg⁻¹ and 40,000 mg · kg⁻¹ in soils increased the root specific surface area comparing with the controls. Additionally, the metabolic analysis showed that root metabolism changed to different degrees under the stress of TPHs, and the levels or species of metabolites had a significant change (P < 0.001). Furthermore, the results showed that 5 of 11 metabolites (VIP value > 1.2) with the root specific surface area from the PLS-DA model analysis, including ethanedioic acid, lactic acid, 2-butenedioic acid, phosphate and propanedioic acid, were positively correlated with the root specific surface area, but the others, gluconic acid, uridine, butanoic acid, maltose, 9,12-octadecadienoic acid, phenylalanine, were negatively correlated with it. In conclusion, using Iris pseudacorus L. to remediate petroleum contaminated soils is feasible, and the metabolic analysis in roots is useful to better understand the metabolic response of plants exposure to petroleum contaminated soils, and then reveals its remediated mechanisms.

A comparison of thermocouple and infrared thermographic analysis of temperature rise on the root surface during the continuous wave of condensation technique.

PubMed

Mc Cullagh, J J; Setchell, D J; Gulabivala, K; Hussey, D L; Biagioni, P; Lamey, P J; Bailey, G

2000-07-01

This study was designed to use two methods of temperature measurement to analyse and quantify the in vitro root surface temperature changes during the initial stage of the continuous wave technique of obturation of 17 single-rooted premolar teeth with standard canal preparations. A model was designed to allow simultaneous temperature measurement with both thermocouples and an infrared thermal imaging system. Two thermocouples were placed on the root surface, one coronally and the other near the root apex. A series of thermal images were recorded by an infrared thermal imaging camera during the downpack procedure. The mean temperature rises on the root surface, as measured by the two thermocouples, averaged 13.9 degrees C over the period of study, whilst the infrared thermal imaging system measured an average rise of 28.4 degrees C at the same sites. Temperatures at the more apical point were higher than those measured coronally. After the first wave of condensation, the second activation of the plugger in the canal prior to its removal always resulted in a secondary rise in temperature. The thermal imaging system detected areas of greater temperature change distant from the two selected thermocouple sites. The continuous wave technique of obturation may result in high temperatures on the external root surface. Infrared thermography is a useful device for mapping patterns of temperature change over a large area.

Simulating root-induced rhizosphere deformation and its effect on water flow

NASA Astrophysics Data System (ADS)

Aravena, J. E.; Ruiz, S.; Mandava, A.; Regentova, E. E.; Ghezzehei, T.; Berli, M.; Tyler, S. W.

2011-12-01

Soil structure in the rhizosphere is influenced by root activities, such as mucilage production, microbial activity and root growth. Root growth alters soil structure by moving and deforming soil aggregates, affecting water and nutrient flow from the bulk soil to the root surface. In this study, we utilized synchrotron X-ray micro-tomography (XMT) and finite element analysis to quantify the effect of root-induced compaction on water flow through the rhizosphere to the root surface. In a first step, finite element meshes of structured soil around the root were created by processing rhizosphere XMT images. Then, soil deformation by root expansion was simulated using COMSOL Multiphysics° (Version 4.2) considering the soil an elasto-plastic porous material. Finally, fluid flow simulations were carried out on the deformed mesh to quantify the effect of root-induced compaction on water flow to the root surface. We found a 31% increase in water flow from the bulk soil to the root due to a 56% increase in root diameter. Simulations also show that the increase of root-soil contact area was the dominating factor with respect to the calculated increase in water flow. Increase of inter-aggregate contacts in size and number were observed within a couple of root diameters away from the root surface. But their influence on water flow was, in this case, rather limited compared to the immediate soil-root contact.

The effect of root surface conditioning on smear layer removal in periodontal regeneration (a scanning electron microscopic study)

NASA Astrophysics Data System (ADS)

Fidyawati, D.; Soeroso, Y.; Masulili, S. L. C.

2017-08-01

The role of root surface conditioning treatment on smear layer removal of human teeth is affected by periodontitis in periodontal regeneration. The objective of this study is to analyze the smear layer on root surface conditioned with 2.1% minocycline HCl ointment (Periocline), and 24% EDTA gel (Prefgel). A total of 10 human teeth indicated for extraction due to chronic periodontitis were collected and root planed. The teeth were sectioned in thirds of the cervical area, providing 30 samples that were divided into three groups - minocycline ointment treatment, 24% EDTA gel treatment, and saline as a control. The samples were examined by scanning electron microscope. No significant differences in levels of smear layer were observed between the minocycline group and the EDTA group (p=0.759). However, there were significant differences in the level of smear layer after root surface treatment in the minocycline and EDTA groups, compared with the control group (p=0.00). There was a relationship between root surface conditioning treatment and smear layer levels following root planing.

Comparison of automatic and visual methods used for image segmentation in Endodontics: a microCT study.

PubMed

Queiroz, Polyane Mazucatto; Rovaris, Karla; Santaella, Gustavo Machado; Haiter-Neto, Francisco; Freitas, Deborah Queiroz

2017-01-01

To calculate root canal volume and surface area in microCT images, an image segmentation by selecting threshold values is required, which can be determined by visual or automatic methods. Visual determination is influenced by the operator's visual acuity, while the automatic method is done entirely by computer algorithms. To compare between visual and automatic segmentation, and to determine the influence of the operator's visual acuity on the reproducibility of root canal volume and area measurements. Images from 31 extracted human anterior teeth were scanned with a μCT scanner. Three experienced examiners performed visual image segmentation, and threshold values were recorded. Automatic segmentation was done using the "Automatic Threshold Tool" available in the dedicated software provided by the scanner's manufacturer. Volume and area measurements were performed using the threshold values determined both visually and automatically. The paired Student's t-test showed no significant difference between visual and automatic segmentation methods regarding root canal volume measurements (p=0.93) and root canal surface (p=0.79). Although visual and automatic segmentation methods can be used to determine the threshold and calculate root canal volume and surface, the automatic method may be the most suitable for ensuring the reproducibility of threshold determination.

Quantitative evaluation of root canal surface roughness after filing with adaptive reciprocating and continuous rotary instruments.

PubMed

Sakhaei Manesh, Vahid; Giacomin, Paul; Stoll, Richard

2017-06-01

Obtaining clean and smooth root canal walls is the ideal clinical outcome of the cleaning and shaping stage in root canal treatment. This study compares the surface roughness of root canal surfaces instrumented with a NiTi filing system with either adaptive reciprocating (AR) or continuous rotation (CR). Root canal cleaning and shaping was carried out on the mesial canals of 24 extracted first molars roots with either AR or CR. Roots were split in half and the surface roughness of their canals was evaluated in 12 three dimensional roughness reconstructions using a scanning electron microscope. Rz (nm) values were calculated in three areas of each reconstruction and analyzed (α = 0.05). Mann-Whitney tests showed that surface roughness was significantly higher overall in the AR group (Rz = 967 ± 250 nm) compared with the CR group (Rz = 739 ± 239 nm; p = 0.044). The roughness values generally increased from apical towards the coronal third in both groups. A less aggressive finishing file or a continuous rotary system to end the cleaning and shaping stage may be beneficial to reduce roughness of the root canal surface. © 2017 Wiley Periodicals, Inc.

Waterlogging-induced changes in root architecture of germplasm accessions of the tropical forage grass Brachiaria humidicola.

PubMed

Cardoso, Juan Andrés; Jiménez, Juan de la Cruz; Rao, Idupulapati M

2014-04-08

Waterlogging is one of the major factors limiting the productivity of pastures in the humid tropics. Brachiaria humidicola is a forage grass commonly used in zones prone to temporary waterlogging. Brachiaria humidicola accessions adapt to waterlogging by increasing aerenchyma in nodal roots above constitutive levels to improve oxygenation of root tissues. In some accessions, waterlogging reduces the number of lateral roots developed from main root axes. Waterlogging-induced reduction of lateral roots could be of adaptive value as lateral roots consume oxygen supplied from above ground via their parent root. However, a reduction in lateral root development could also be detrimental by decreasing the surface area for nutrient and water absorption. To examine the impact of waterlogging on lateral root development, an outdoor study was conducted to test differences in vertical root distribution (in terms of dry mass and length) and the proportion of lateral roots to the total root system (sum of nodal and lateral roots) down the soil profile under drained or waterlogged soil conditions. Plant material consisted of 12 B. humidicola accessions from the gene bank of the International Center for Tropical Agriculture, Colombia. Rooting depth was restricted by 21 days of waterlogging and confined to the first 30 cm below the soil surface. Although waterlogging reduced the overall proportion of lateral roots, its proportion significantly increased in the top 10 cm of the soil. This suggests that soil flooding increases lateral root proliferation of B. humidicola in the upper soil layers. This may compensate for the reduction of root surface area brought about by the restriction of root growth at depths below 30 cm. Further work is needed to test the relative efficiency of nodal and lateral roots for nutrient and water uptake under waterlogged soil conditions. Published by Oxford University Press on behalf of the Annals of Botany Company.

Nitrogen metabolism of two contrasting poplar species during acclimation to limiting nitrogen availability

PubMed Central

Luo, Zhi-Bin

2013-01-01

To investigate N metabolism of two contrasting Populus species in acclimation to low N availability, saplings of slow-growing species (Populus popularis, Pp) and a fast-growing species (Populus alba × Populus glandulosa, Pg) were exposed to 10, 100, or 1000 μM NH4NO3. Despite greater root biomass and fine root surface area in Pp, lower net influxes of NH4 + and NO3 – at the root surface were detected in Pp compared to those in Pg, corresponding well to lower NH4 + and NO3 – content and total N concentration in Pp roots. Meanwhile, higher stable N isotope composition (δ15N) in roots and stronger responsiveness of transcriptional regulation of 18 genes involved in N metabolism were found in roots and leaves of Pp compared to those of Pg. These results indicate that the N metabolism of Pp is more sensitive to decreasing N availability than that of Pg. In both species, low N treatments decreased net influxes of NH4 + and NO3 –, root NH4 + and foliar NO3 – content, root NR activities, total N concentration in roots and leaves, and transcript levels of most ammonium (AMTs) and nitrate (NRTs) transporter genes in leaves and genes involved in N assimilation in roots and leaves. Low N availability increased fine root surface area, foliar starch concentration, δ15N in roots and leaves, and transcript abundance of several AMTs (e.g. AMT1;2) and NRTs (e.g. NRT1;2 and NRT2;4B) in roots of both species. These data indicate that poplar species slow down processes of N acquisition and assimilation in acclimation to limiting N supply. PMID:23963674

Numerical Prediction of the Influence of Process Parameters on Large Area Diamond Deposition by DC Arcjet with ARC Roots Rotating and Operating at Gas Recycling Mode

NASA Astrophysics Data System (ADS)

Lu, F. X.; Huang, T. B.; Tang, W. Z.; Song, J. H.; Tong, Y. M.

A computer model have been set up for simulation of the flow and temperature field, and the radial distribution of atomic hydrogen and active carbonaceous species over a large area substrate surface for a new type dc arc plasma torch with rotating arc roots and operating at gas recycling mode A gas recycling radio of 90% was assumed. In numerical calculation of plasma chemistry, the Thermal-Calc program and a powerful thermodynamic database were employed. Numerical calculations to the computer model were performed using boundary conditions close to the experimental setup for large area diamond films deposition. The results showed that the flow and temperature field over substrate surface of Φ60-100mm were smooth and uniform. Calculations were also made with plasma of the same geometry but no arc roots rotation. It was clearly demonstrated that the design of rotating arc roots was advantageous for high quality uniform deposition of large area diamond films. Theoretical predictions on growth rate and film quality as well as their radial uniformity, and the influence of process parameters on large area diamond deposition were discussed in detail based on the spatial distribution of atomic hydrogen and the carbonaceous species in the plasma over the substrate surface obtained from thermodynamic calculations of plasma chemistry, and were compared with experimental observations.

Phytotoxic substances in runoff from forested catchment areas

NASA Astrophysics Data System (ADS)

Grimvall, Anders; Bengtsson, Maj-Britt; Borén, Hans; Wahlström, Dan

Runoff from different catchment areas in southern Sweden was tested in a root bioassay based on solution cultures of cucumber seedlings. Water samples from agricultural catchment areas produced no signs at all or only weak signs of inhibited root growth, whereas several water samples from catchment areas dominated by mires or coniferous forests produced visible root injuries. The most severe root injuries (very short roots, discolouration, swelling of root tips and lack of root hairs) were caused by samples from a catchment area without local emissions and dominated by old stands of spruce. Fractionation by ultrafiltration showed that the phytotoxic effect of these samples could be attributed to organic matter with a nominal molecular-weight exceeding 1000 or to substances associated with organic macromolecules. Experiments aimed at concentrating phytotoxic compounds from surface water indicated that the observed growth inhibition was caused by strongly hydrophilic substances. Previous reports on phytotoxic, organic substances of natural origin have emphasized interaction between plants growing close together. The presence of phytotoxic substances in runoff indicates that there is also a large-scale dispersion of such compounds.

Rice Genotype Differences in Tolerance of Zinc-Deficient Soils: Evidence for the Importance of Root-Induced Changes in the Rhizosphere

PubMed Central

Mori, Asako; Kirk, Guy J. D.; Lee, Jae-Sung; Morete, Mark J.; Nanda, Amrit K.; Johnson-Beebout, Sarah E.; Wissuwa, Matthias

2016-01-01

Zinc (Zn) deficiency is a major constraint to rice production and Zn is also often deficient in humans with rice-based diets. Efforts to breed more Zn-efficient rice are constrained by poor understanding of the mechanisms of tolerance to deficiency. Here we assess the contributions of root growth and root Zn uptake efficiency, and we seek to explain the results in terms of specific mechanisms. We made a field experiment in a highly Zn-deficient rice soil in the Philippines with deficiency-tolerant and -sensitive genotypes, and measured growth, Zn uptake and root development. We also measured the effect of planting density. Tolerant genotypes produced more crown roots per plant and had greater uptake rates per unit root surface area; the latter was at least as important as root number to overall tolerance. Tolerant and sensitive genotypes took up more Zn per plant at greater planting densities. The greater uptake per unit root surface area, and the planting density effect can only be explained by root-induced changes in the rhizosphere, either solubilizing Zn, or neutralizing a toxin that impedes Zn uptake (possibly HCO3− or Fe2+), or both. Traits for these and crown root number are potential breeding targets. PMID:26793198

Rice Genotype Differences in Tolerance of Zinc-Deficient Soils: Evidence for the Importance of Root-Induced Changes in the Rhizosphere.

PubMed

Mori, Asako; Kirk, Guy J D; Lee, Jae-Sung; Morete, Mark J; Nanda, Amrit K; Johnson-Beebout, Sarah E; Wissuwa, Matthias

2015-01-01

Zinc (Zn) deficiency is a major constraint to rice production and Zn is also often deficient in humans with rice-based diets. Efforts to breed more Zn-efficient rice are constrained by poor understanding of the mechanisms of tolerance to deficiency. Here we assess the contributions of root growth and root Zn uptake efficiency, and we seek to explain the results in terms of specific mechanisms. We made a field experiment in a highly Zn-deficient rice soil in the Philippines with deficiency-tolerant and -sensitive genotypes, and measured growth, Zn uptake and root development. We also measured the effect of planting density. Tolerant genotypes produced more crown roots per plant and had greater uptake rates per unit root surface area; the latter was at least as important as root number to overall tolerance. Tolerant and sensitive genotypes took up more Zn per plant at greater planting densities. The greater uptake per unit root surface area, and the planting density effect can only be explained by root-induced changes in the rhizosphere, either solubilizing Zn, or neutralizing a toxin that impedes Zn uptake (possibly [Formula: see text] or Fe(2+)), or both. Traits for these and crown root number are potential breeding targets.

Effect of EDTA, sodium hypochlorite, and chlorhexidine gluconate with or without surface modifiers on dentin microhardness.

PubMed

Aslantas, Eda E; Buzoglu, Hatice Dogan; Altundasar, Emre; Serper, Ahmet

2014-06-01

This study aimed to evaluate the effects of root canal irrigants on the microhardness of root canal dentin in the presence and absence of surface-modifying agents. Forty-eight root halves were prepared by longitudinal splitting of the distal roots of 24 freshly extracted mandibular human third molars and embedded in autopolymerizing acrylic resin, leaving the dentin surface exposed. After polishing, the microhardness values of the untreated dentin surfaces were recorded by using Vickers tester at the mid-root level. The root halves were randomly assigned to 6 groups composed of 8 samples each and treated for 5 minutes with one of the following irrigants: 17% EDTA, REDTA, 2% chlorhexidine gluconate (CHX), 2% CHX with surface modifiers (CHX-Plus), 6% NaOCl, or 6% NaOCl with surface modifiers (Chlor-XTRA). After surface treatment, dentin microhardness values were recorded at close proximity to the initial indentation areas. Experimental data were statistically analyzed by using the t test and one-way analysis of variance, followed by Tukey honestly significant difference test at α = 0.05. EDTA, REDTA, NaOCl, and Chlor-XTRA significantly decreased the microhardness of root dentin compared with intact controls (P < .05). The addition of surface modifiers to the irrigants did not affect the microhardness of the samples. Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Molecular Analysis of SCARECROW Genes Expressed in White Lupin Cluster Roots

USDA-ARS?s Scientific Manuscript database

The Scarecrow (SCR) transcription factor plays a crucial role in root cell radial patterning and is required for maintenance of the quiescent center and differentiation of the endodermis. In response to phosphorus (P) deficiency, white lupin (Lupinus albus L.) root surface area increases some 50- to...

Micro-CT analyses of apical enlargement and molar root canal complexity.

PubMed

Markvart, M; Darvann, T A; Larsen, P; Dalstra, M; Kreiborg, S; Bjørndal, L

2012-03-01

To compare the effectiveness of two rotary hybrid instrumentation techniques with focus on apical enlargement in molar teeth and to quantify and visualize spatial details of instrumentation efficacy in root canals of different complexity. Maxillary and mandibular molar teeth were scanned using X-ray microcomputed tomography. Root canals were prepared using either a GT/Profile protocol or a RaCe/NiTi protocol. Variables used for evaluation were the following: distance between root canal surfaces before and after preparation (distance after preparation, DAP), percentage of root canal area remaining unprepared and increase in canal volume after preparation. Root canals were classified according to size and complexity, and consequences of unprepared portions of narrow root canals and intraradicular connections/isthmuses were included in the analyses. One- and two-way anova were used in the statistical analyses. No difference was found between the two techniques: DAP(apical-third) (P = 0.590), area unprepared(apical-third) (P = 0.126) and volume increase(apical-third) (P = 0.821). Unprepared root canal area became larger in relation to root canal size and complexity, irrespective of the technique used. Percentage of root canal area remaining unprepared was significantly lower in small root canals and complex systems compared to large root canals. The isthmus area per se contributed with a mean of 17.6%, and with a mean of 25.7%, when a narrow root canal remained unprepared. The addition of isthmuses did not significantly alter the ratio of instrumented to unprepared areas at total root canal level. Distal and palatal root canals had the highest level of unprepared area irrespective of the two instrumentation techniques examined. © 2011 International Endodontic Journal.

Incidence and severity of root resorption in orthodontically moved premolars in dogs.

PubMed

Maltha, J C; van Leeuwen, E J; Dijkman, G E H M; Kuijpers-Jagtman, A M

2004-05-01

To study treatment-related factors for external root resorption during orthodontic tooth movement. An experimental animal study. Department of Orthodontics and Oral Biology, University Medical Centre Nijmegen, The Netherlands. Twenty-four young adult beagle dogs. Mandibular premolars were bodily moved with continuous or intermittent controlled orthodontic forces of 10, 25, 50, 100, or 200 cN according to standardized protocols. At different points in time histomorphometry was performed to determine the severity of root resorption. Prevalence of root resorptions, defined as microscopically visible resorption lacunae in the dentin. Severity of resorption was defined by the length, relative length, depth, and surface area of each resorption area. The incidence of root resorption increased with the duration of force application. After 14-17 weeks of force application root resorption was found at 94% of the root surfaces at pressure sides. The effect of force magnitude on the severity of root resorption was not statistically significant. The severity of root resorption was highly related to the force regimen. Continuous forces caused significantly more severe root resorption than intermittent forces. A strong correlation (0.60 < r < 0.68) was found between the amount of tooth movement and the severity of root resorption. Root resorption increases with the duration of force application. The more teeth are displaced, the more root resorption will occur. Intermittent forces cause less severe root resorption than continuous forces, and force magnitude is probably not decisive for root resorption.

Development of partial rock veneers by root throw in a subalpine setting

USGS Publications Warehouse

Osterkamp, W.R.; Toy, T.J.; Lenart, M.T.

2006-01-01

Rock veneers stabilize hillslope surfaces, occur especially in areas of immature soil, and form through a variety of process sets that includes root throw. Near Westcliffe, Colorado, USA, data were collected from a 20 ?? 500 m transect on the east slope of the Sangre de Cristo Mountains. Ages of pit/mound complexes with rock fragments exposed at the surface by root throw ranged from recent (freshly toppled tree) to unknown (complete tree decay). Calculations based on dimensions of the pit/mound complexes, estimated time of free topppling, sizes of exposed rock fragments, and percentage rock covers at pit/mound complexes, as well as within the transect area, indicate that recent rates of root throw have resulted in only partial rock veneering since late Pleistocene deglaciation. Weathering of rock fragments prevent development of an extensive rock veneer and causes a balance, achieved within an estimated 700 years, between the rates of rock-fragment exposure by root throw and clast disintegration by chemical reduction. The estimated rate of rock-fragment reduction accounts for part of the fluvial sediment yields observed for forested subalpine areas of western North America. Copyright ?? 2005 John Wiley & Sons, Ltd.

Efficacy of a new filler-containing root coating material for dentin remineralization.

PubMed

Okuyama, Katsushi; Kadowaki, Yoshitaka; Matsuda, Yasuhiro; Hashimoto, Naoki; Oki, Saiko; Yamamoto, Hiroko; Tamaki, Yukimichi; Sano, Hidehiko

2016-08-01

To evaluate a new root coating material containing surface pre-reacted glass-ionomer (S-PRG) filler for remineralization of demineralized dentin. The dentin was exposed on root surfaces of human third molars and demineralized by immersion in demineralization solution for 4 days. The demineralized dentin surface was divided into three areas. The center area was left untreated. The area on one side of the center area was coated with protective wax. The area on the other side was coated with one of four test materials: fluoride-containing S-PRG filler (PRG Barrier Coat: PR), fluoride-containing bonding agent (Bond Force: BF), fluoride-containing glass-ionomer cement as a positive control (Fuji IX EXTRA: EX), or non-fluoride-containing bonding agent as a negative control (Clearfil MegaBond: MB). The samples were stored in remineralization solution for 7 days, and then cut into two slices. The mineral changes, defined as variation in mineral loss between wax-coated area and the central untreated area, were measured in one slice by transversal microradiography. The fluoride concentration was measured in the other slice by µ-particle-induced gamma/X-ray emission. Seven thin specimens (0.25-mm thickness) of each test material were used to determine fluoride ion release from the materials over 21 days. The mineral changes were greatest for EX, followed by PR, with no difference between BF and MB (P> 0.05). Regarding the fluoride concentrations in dentin, there was no difference between EX and PR (P> 0.05). MB had the lowest value (P< 0.01). Fluoride release from EX was largest, followed by PR, with BF showing low fluoride release (P< 0.05). MB had no fluoride release. A new coating material with S-PRG filler can be applied in a thin layer on root dentin, which could be especially useful for hard-to-access lesions. This material remineralized demineralized root dentin and had fluoride diffusion characteristics similar to those of glass-ionomer cement in vitro.

Comparative evaluation of root canal preparations of maxillary first molars with self-adjusting file, reciproc single file, and revo-s rotary file: A micro-computed tomography study.

PubMed

Ahmetoglu, Fuat; Keles, Ali; Simsek, Neslihan; Ocak, M Sinan; Yologlu, Saim

2015-01-01

This study was aimed to use micro-computed tomography (μ-CT) to evaluate the canal shaping properties of three nickel-titanium instruments, Self-Adjusting File (SAF), Reciproc, and Revo-S rotary file, in maxillary first molars. Thirty maxillary molars were scanned preoperatively by using micro-computed tomography (μ-CT) scans at 13,68 μm resolution. The teeth were randomly assigned to three groups (n = 10). The root canals were shaped with SAF, Reciproc, and Revo-S, respectively. The shaped root canals were rescanned. Changes in canal volumes and surface areas were compared with preoperative values. The data were analyzed using Kruskal-Wallis and Conover's post hoc tests, with p < .05 denoting a statistically significant difference. Preoperatively canal volumes and surface area were statistically similar among the three groups (p > .05). There were statistically significant differences in all measures comparing preoperative and postoperative canal models (p = 0.0001). These differences occurred after instrumentation among the three experimental groups showed no statistically significant difference for volume (p > .05). Surface area showed the similar activity in buccal canals in each of the three techniques whereas no statistically significant difference was detected among surface area, the SAF, and the Revo-S in the palatal (P) canal. Each of three shaping system showed the similar volume activity in all canals, but SAF and Revo-S provided more effectively root planning in comparison with Reciproc in P canal. © Wiley Periodicals, Inc.

Ultramorphology of the root surface subsequent to hand-ultrasonic simultaneous instrumentation during non-surgical periodontal treatments. An in vitro study

PubMed Central

D. ASPRIELLO, Simone; PIEMONTESE, Matteo; LEVRINI, Luca; SAURO, Salvatore

2011-01-01

Objective The purpose of this study was to investigate the ultramorphology of the root surfaces induced by mechanical instrumentation performed using conventional curettes or piezoelectric scalers when used single-handedly or with a combined technique. Material and Methods Thirty single-rooted teeth were selected and divided into 3 groups: Group A, instrumentation with curettes; Group B instrumentation with titanium nitride coated periodontal tip mounted in a piezoelectric handpiece; Group C, combined technique with curette/ultrasonic piezoelectric instrumentation. The specimens were processed and analyzed using confocal and scanning electron microscopy. Differences between the different groups of instrumentation were determined using Pearson’s χ 2 with significance predetermined at α=0.001. Results Periodontal scaling and root planing performed with curettes, ultrasonic or combined instrumentation induced several morphological changes on the root surface. The curettes produced a compact and thick multilayered smear layer, while the morphology of the root surfaces after ultrasonic scaler treatment appeared irregular with few grooves and a thin smear layer. The combination of curette/ultrasonic instrumentation showed exposed root dentin tubules with a surface morphology characterized by the presence of very few grooves and slender remnants of smear layer which only partially covered the root dentin. In some cases, it was also possible to observe areas with exposed collagen fibrils. Conclusion The curette-ultrasonic simultaneous instrumentation may combine the beneficial effects of each instrument in a single technique creating a root surface relatively free from the physical barrier of smear layer and dentin tubules orifices partial occlusion. PMID:21437474

[Effects of arbuscular mycorrhizal fungi on root system morphology and sucrose and glucose contents of Poncirus trifoliata].

PubMed

Zou, Ying-Ning; Wu, Qiang-Sheng; Li, Yan; Huang, Yong-Ming

2014-04-01

The effects of inoculation with Glomus mosseae, G. versiforme, and their mixture on plant growth, root system morphology, and sucrose and glucose contents of trifoliate orange (Poncirus trifoliata L.) were studied by pot culture. The results showed that all the inoculated treatments significantly increased the plant height, stem diameter, leaf number, and shoot and root biomass. In addition, the mycorrhizal treatments significantly increased the number of 1st, 2nd, and 3rd lateral roots. Inoculation with arbuscular mycorrhizal fungi significantly increased the root projected area, surface area, volume, and total root length (mainly 0-1 cm root length), but decreased the root average diameter. Meanwhile, G. versiforme showed the best effects. Mycorrhizal inoculation significantly increased the leaf sucrose and root glucose contents, but decreased the leaf glucose and root sucrose contents. Owing to the 'mycorrhizal carbon pool' in roots, inoculation with arbuscular mycorrhizal fungi resulted in high glucose content and low sucrose content of roots, which would facilitate the root growth and development, thereby the establishment of better root system morphology of host plants.

Effects of erbium, chromium:YSGG laser irradiation on root surface: morphological and atomic analytical studies.

PubMed

Kimura, Y; Yu, D G; Kinoshita, J; Hossain, M; Yokoyama, K; Murakami, Y; Nomura, K; Takamura, R; Matsumoto, K

2001-04-01

The purpose of this study was to investigate the morphological and atomic changes on the root surface by stereoscopy, field emission-scanning electron microscopy (FE-SEM), and energy dispersive X-ray spectroscopy (SEM-EDX) after erbium, chromium:yttrium, scandium, gallium, garnet (Er,Cr:YSGG) laser irradiation in vitro. There have been few reports on morphological and atomic analytical study on root surface by Er,Cr:YSGG laser irradiation. Eighteen extracted human premolar and molar teeth were irradiated on root surfaces at a vertical position with water-air spray by an Er,Cr:YSGG laser at the parameter of 5.0 W and 20 Hz for 5 sec while moving. The samples were then morphologically observed by stereoscopy and FE-SEM and examined atomic-analytically by SEM-EDX. Craters having rough but clean surfaces and no melting or carbonization were observed in the samples. An atomic analytical examination showed that the calcium ratio to phosphorus showed no significant changes between the control and irradiated areas (p > 0.01). These results showed that the Er,Cr:YSGG laser has a good cutting effect on root surface and causes no burning or melting after laser irradiation.

The effect of a manual instrumentation technique on five types of premolar root canal geometry assessed by microcomputed tomography and three-dimensional reconstruction

PubMed Central

2011-01-01

Background Together with diagnosis and treatment planning, a good knowledge of the root canal system and its frequent variations is a necessity for successful root canal therapy. The selection of instrumentation techniques for variants in internal anatomy of teeth has significant effects on the shaping ability and cleaning effectiveness. The aim of this study was to reveal the differences made by including variations in the internal anatomy of premolars into the study protocol for investigation of a single instrumentation technique (hand ProTaper instruments) assessed by microcomputed tomography and three-dimensional reconstruction. Methods Five single-root premolars, whose root canal systems were classified into one of five types, were scanned with micro-CT before and after preparation with a hand ProTaper instrument. Instrumentation characteristics were measured quantitatively in 3-D using a customized application framework based on MeVisLab. Numeric values were obtained for canal surface area, volume, volume changes, percentage of untouched surface, dentin wall thickness, and the thickness of dentin removed. Preparation errors were also evaluated using a color-coded reconstruction. Results Canal volumes and surface areas were increased after instrumentation. Prepared canals of all five types were straightened, with transportation toward the inner aspects of S-shaped or multiple curves. However, a ledge was formed at the apical third curve of the type II canal system and a wide range in the percentage of unchanged canal surfaces (27.4-83.0%) was recorded. The dentin walls were more than 0.3 mm thick except in a 1 mm zone from the apical surface and the hazardous area of the type II canal system after preparation with an F3 instrument. Conclusions The 3-D color-coded images showed different morphological changes in the five types of root canal systems shaped with the same hand instrumentation technique. Premolars are among the most complex teeth for root canal treatment and instrumentation techniques for the root canal systems of premolars should be selected individually depending on the 3-D canal configuration of each tooth. Further study is needed to demonstrate the differences made by including variations in the internal anatomy of teeth into the study protocol of clinical RCT for identifying the best preparation technique. PMID:21676233

How does vineyard management intensity affect inter-row plant diversity and associated root parameters

NASA Astrophysics Data System (ADS)

Winter, Silvia; Labuda, Thomas; Probus, Sandra; Penke, Nicole; Himmelbauer, Margarita; Loiskandl, Willibald; Strauss, Peter; Bauer, Thomas; Popescu, Daniela; Comsa, Maria; Bunea, Claudiu-Ioan; Zaller, Johann G.; Kriechbaum, Monika

2017-04-01

Vineyard management has changed dramatically in the last 50 years. In many wine-growing regions, vineyard inter-rows are kept clean of vegetation by frequent tillage or use of herbicides to establish bare soil systems. In the last thirty years, policy-makers and several winegrowers have realized that temporary or permanent vegetation cover between the vine rows may increase ecosystem services like soil erosion mitigation, soil fertility and biodiversity conservation. The inter-row area of a vineyard can host a diverse flora providing habitat and food resources for pollinating insects and natural enemies of pests. The goal of this study was to analyze the influence of different soil management intensities on plant diversity and root parameters in the vineyard inter-rows. We investigated 15 vineyards in Romania and 14 in Austria to study the effects of three different management intensities on plant diversity, above and below-ground plant biomass, total root length and surface area of roots. Management intensity ranged from bare soil inter-rows to alternative soil tillage every second year to permanent vegetation cover for more than five years. In each vineyard inter-row, six soil samples (7 cm diameter and 10 cm height) of the upper soil layer were extracted for root analyses. Root were separated from the soil, stained and finally scanned and analyzed with the WinRHIZO software. Finally, roots were dried at 70°C to obtain dry matter of the root samples. Vegetation cover and vascular plant diversity was recorded in four 1 m2 plots within each vineyard inter-row two times a year. The most intensive bare soil management regime in Romania significantly reduced root biomass, total root length and surface area in comparison to the alternative and permanent vegetation cover management. Plant biodiversity was also reduced by intensive management, but differences were not significant. While alternative tillage every second year showed the highest values of plant species diversity and functional richness, total root length, surface area and root biomass always showed the highest value in the vineyards with permanent vegetation cover. In Austria, the difference between temporary and permanent vegetation cover was much less pronounced than in Romania. The overall synthesis of these results combined with additional biodiversity datasets and soil parameters gathered within the transdisciplinary BiodivERsA project VineDivers will be used to draft management and policy recommendations for various stakeholder groups engaged in viticulture.

MicroCT analysis of a retrieved root restored with a bonded fiber-reinforced composite dowel: a pilot study.

PubMed

Lorenzoni, Fabio Cesar; Bonfante, Estevam A; Bonfante, Gerson; Martins, Leandro M; Witek, Lukasz; Silva, Nelson R F A

2013-08-01

This evaluation aimed to (1) validate micro-computed tomography (microCT) findings using scanning electron microscopy (SEM) imaging, and (2) quantify the volume of voids and the bonded surface area resulting from fiber-reinforced composite (FRC) dowel cementation technique using microCT scanning technology/3D reconstructing software. A fiberglass dowel was cemented in a condemned maxillary lateral incisor prior to its extraction. A microCT scan was performed of the extracted tooth creating a large volume of data in DICOM format. This set of images was imported to image-processing software to inspect the internal architecture of structures. The outer surface and the spatial relationship of dentin, FRC dowel, cement layer, and voids were reconstructed. Three-dimensional spatial architecture of structures and volumetric analysis revealed that 9.89% of the resin cement was composed of voids and that the bonded area between root dentin and cement was 60.63% larger than that between cement and FRC dowel. SEM imaging demonstrated the presence of voids similarly observed using microCT technology (aim 1). MicroCT technology was able to nondestructively measure the volume of voids within the cement layer and the bonded surface area at the root/cement/FRC interfaces (aim 2). The interfaces at the root dentin/cement/dowel represent a timely and relevant topic where several efforts have been conducted in the past few years to understand their inherent features. MicroCT technology combined with 3D reconstruction allows for not only inspecting the internal arrangement rendered by fiberglass adhesively bonded to root dentin, but also estimating the volume of voids and contacted bond area between the dentin and cement layer. © 2013 by the American College of Prosthodontists.

Mixed nano/micro-sized calcium phosphate composite and EDTA root surface etching improve availability of graft material in intrabony defects: an in vivo scanning electron microscopy evaluation.

PubMed

Gamal, Ahmed Y; Iacono, Vincent J

2013-12-01

The use of nanoparticles of graft materials may lead to breakthrough applications for periodontal regeneration. However, due to their small particle size, nanoparticles may be eliminated from periodontal defects by phagocytosis. In an attempt to improve nanoparticle retention in periodontal defects, the present in vivo study uses scanning electron microscopy (SEM) to evaluate the potential of micrograft particles of β-tricalcium phosphate (β-TCP) to enhance the binding and retention of nanoparticles of hydroxyapatite (nHA) on EDTA-treated and non-treated root surfaces in periodontal defects after 14 days of healing. Sixty patients having at least two hopeless periodontally affected teeth designated for extraction were randomly divided into four treatment groups (15 patients per group). Patients in group 1 had selected periodontal intrabony defects grafted with nHA of particle size 10 to 100 nm. Patients in group 2 were treated in a similar manner but had the affected roots etched for 2 minutes with a neutral 24% EDTA gel before grafting of the associated vertical defects with nHA. Patients in group 3 had the selected intrabony defects grafted with a composite graft consisting of equal volumes of nHA and β-TCP (particle size 63 to 150 nm). Patients in group 4 were treated as in group 3 but the affected roots were etched with neutral 24% EDTA as in group 2. For each of the four groups, one tooth was extracted immediately, and the second tooth was extracted after 14 days of healing for SEM evaluation. Fourteen days after surgery, all group 1 samples were devoid of any nanoparticles adherent to the root surfaces. Group 2 showed root surface areas 44.7% covered by a single layer of clot-blended grafted particles 14 days following graft application. After 14 days, group 3 samples appeared to retain fibrin strands devoid of grafted particles. Immediately extracted root samples of group 4 had adherent graft particles that covered a considerable area of the root surfaces (88.6%). Grafted particles appeared to cover all samples in a multilayered pattern. After 14 days, the group 4 extracted samples showed multilayered fibrin-covered nano/micro-sized graft particles adherent to the root surfaces (78.5%). The use of a composite graft consisting of nHA and microsized β-TCP after root surface treatment with 24% EDTA may be a suitable method to improve nHA retention in periodontal defects with subsequent graft bioreactivity.

A scanning electron microscopy study of diseased root surfaces conditioned with EDTA gel plus Cetavlon after scaling and root planing.

PubMed

Martins Júnior, Walter; De Rossi, Andiara; Samih Georges Abi Rached, Ricardo; Rossi, Marcos Antonio

2011-01-01

In the present investigation, a scanning electron microscopy analysis was performed to evaluate the effects of the topical application of ethylenediaminetetraacetic acid (EDTA) gel associated with Cetavlon (EDTAC) in removing the smear layer and exposing collagen fibers following root surface instrumentation. Twenty-eight teeth from adult humans, single rooted and scheduled for extraction due to periodontal reasons, were selected. Each tooth was submitted to manual (scaling and root planing) instrumentation alone or combined with ultrasonic instruments, with or without etching using a 24% EDTAC gel. Following extraction, specimens were processed and examined under a scanning electron microscope. A comparative morphological semi-quantitative analysis was performed; the intensity of the smear layer and the decalcification of cementum and dentinal surfaces were graded in 12 sets using an arbitrary scale ranging from 1 (area covered by a smear layer) to 4 (no smear layer). Root debridement with hand instruments alone or combined with ultrasonic instruments resulted in a similar smear layer covering the root surfaces. The smear layer was successfully removed from the surfaces treated with EDTAC, which exhibited numerous exposed dentinal tubules and collagen fibers. This study supports the hypothesis that manual instrumentation alone or instrumentation combined with ultrasonic instrumentation is unable to remove the smear layer, whereas the subsequent topical application of EDTAC gel effectively removes the smear layer, uncovers dentinal openings and exposes collagen fibers.

Does apical root resection in endodontic microsurgery jeopardize the prosthodontic prognosis?

PubMed Central

Cho, Sin-Yeon

2013-01-01

Apical surgery cuts off the apical root and the crown-to-root ratio becomes unfavorable. Crown-to-root ratio has been applied to periodontally compromised teeth. Apical root resection is a different matter from periodontal bone loss. The purpose of this paper is to review the validity of crown-to-root ratio in the apically resected teeth. Most roots have conical shape and the root surface area of coronal part is wider than apical part of the same length. Therefore loss of alveolar bone support from apical resection is much less than its linear length.The maximum stress from mastication concentrates on the cervical area and the minimum stress was found on the apical 1/3 area. Therefore apical root resection is not so harmful as periodontal bone loss. Osteotomy for apical resection reduces longitudinal width of the buccal bone and increases the risk of endo-perio communication which leads to failure. Endodontic microsurgery is able to realize 0 degree or shallow bevel and precise length of root resection, and minimize the longitudinal width of osteotomy. The crown-to-root ratio is not valid in evaluating the prosthodontic prognosis of the apically resected teeth. Accurate execution of endodontic microsurgery to preserve the buccal bone is essential to avoid endo-perio communication. PMID:23741707

Root cementum modulates periodontal regeneration in Class III furcation defects treated by the guided tissue regeneration technique: a histometric study in dogs.

PubMed

Gonçalves, Patricia F; Gurgel, Bruno C V; Pimentel, Suzana P; Sallum, Enilson A; Sallum, Antonio W; Casati, Márcio Z; Nociti, Francisco H

2006-06-01

Because the possibility of root cementum preservation as an alternative approach for the treatment of periodontal disease has been demonstrated, this study aimed to histometrically evaluate the effect of root cementum on periodontal regeneration. Bilateral Class III furcation defects were created in dogs, and each dog was randomly assigned to receive one of the following treatments: control (group A): scaling and root planing with the removal of root cementum; or test (group B): removal of soft microbial deposits by polishing the root surface with rubber cups and polishing paste, aiming at maximum cementum preservation. Guided tissue regeneration (GTR) was applied to both groups. Four months after treatment, a superior length of new cementum (3.59 +/- 1.67 mm versus 6.20 +/- 2.26 mm; P = 0.008) and new bone (1.86 +/- 1.76 mm versus 4.62 +/- 3.01 mm; P = 0.002) and less soft tissue along the root surface (2.77 +/- 0.79 mm versus 1.10 +/- 1.48 mm; P = 0.020) was observed for group B. Additionally, group B presented a larger area of new bone (P = 0.004) and a smaller area of soft tissue (P = 0.008). Within the limits of this study, root cementum may modulate the healing pattern obtained by guided tissue regeneration in Class III furcation defects.

Assessing the applicability of the earth impedance method for in situ studies of tree root systems

PubMed Central

Urban, Josef; Bequet, Raphael; Mainiero, Raphael

2011-01-01

Several electrical methods have been introduced as non-invasive techniques to overcome the limited accessibility to root systems. Among them, the earth impedance method (EIM) represents the most recent development. Applying an electrical field between a cormus and the rooted soil, the EIM measures the absorptive root surface area (ARSA) from grounding resistance patterns. Allometric relationships suggested that this method was a valuable tool. Crucial assumptions for the applicability of the EIM, however, have not been tested experimentally. Focusing on tree root systems, the present study assesses the applicability of the EIM. Six hypotheses, deduced from the EIM approach, were tested in several experiments and the results were compared with conventional methods. None of the hypotheses could be verified and the results allow two major conclusions. First, in terms of an analogue electrical circuit, a tree-root–soil continuum appears as a serial circuit with xylem and soil resistance being the dominant components. Allometric variation in contact resistance, with the latter being the proxy for root surface area, are thus overruled by the spatial and seasonal variation of soil and xylem resistances. Second, in a tree-root–soil continuum, distal roots conduct only a negligible portion of the electric charge. Most of charge carriers leave the root system in the proximal parts of the root–soil interface. PMID:21273337

Connective tissue graft vs. emdogain: A new approach to compare the outcomes.

PubMed

Sayar, Ferena; Akhundi, Nasrin; Gholami, Sanaz

2013-01-01

The aim of this clinical trial study was to clinically evaluate the use of enamel matrix protein derivative combined with the coronally positioned flap to treat gingival recession compared to the subepithelial connective tissue graft by a new method to obtain denuded root surface area. Thirteen patients, each with two or more similar bilateral Miller class I or II gingival recession (40 recessions) were randomly assigned to the test (enamel matrix protein derivative + coronally positioned flap) or control group (subepithelial connective tissue graft). Recession depth, width, probing depth, keratinized gingival, and plaque index were recorded at baseline and at one, three, and six months after treatment. A stent was used to measure the denuded root surface area at each examination session. Results were analyzed using Kolmogorov-Smirnov, Wilcoxon, Friedman, paired-sample t test. The average percentages of root coverage for control and test groups were 63.3% and 55%, respectively. Both groups showed significant keratinized gingival increase (P < 0.05). Recession depth decreased significantly in both groups. Root surface area was improved significantly from baseline with no significant difference between the two study groups (P > 0.05). The results of Friedman test were significant for clinical indices (P < 0.05), except for probing depth in control group (P = 0.166). Enamel matrix protein derivative showed the same results as subepithelial connective tissue graft with relatively easy procedure to perform and low patient morbidity.

Role of plant-rock interactions in the N cycle of oligotrophic environments

NASA Astrophysics Data System (ADS)

Gaddis, E. E.; Zaharescu, D. G.; Dontsova, K.; Chorover, J.; Galey, M.; Huxman, T. E.

2013-12-01

The vital role of nitrogen--an abundant, but inaccessible building block for growth--in plants is well known. At the same time, plants and microorganisms are driving forces for accumulation of available N in the soils as they form. A deep understanding of N cycle initiation, progression, and link to ecological systems and their development is therefore necessary. A mesocosm experiment was set up with the goal of exploring the role of interactions between four rock types and biota on N fate in oligotrophic environments. Basalt, rhyolite, granite, and schist were used with 6 treatments: abiotic control; microbes only; grass and microbes; pine and microbes; grass, microbes, and mycorrhizal fungi; and pine, microbes, and mycorrhizal fungi. Pinus ponderosa and Buchloe dactyloides were seeded on the different rock media and maintained with purified air and water but no nutrient additions for 8 month. Throughout the experiment leachate solution was collected and its chemical composition characterized, including organic and inorganic C and N. In addition, plant roots were scanned and their images analyzed to quantify their morphological features. Root parameters included measurements of length, surface area, diameter, volume, the number of tips, forks and links, altitude, and overall plant biomass. Over the 8 month period, there was sustained vegetation growth on all rocks without N addition. A high C:N ratio was seen across all substrates, indicating N deficiency. A strong relationship was observed between total N removal in soil leachate and a number of plant parameters, including plant biomass, total surface area of the roots, sum of the root tips, and total root volume. These relationships were the strongest in basalt, where the pines had higher root surface area than grasses and this was accompanied by higher total N in leachate. There was also a positive correlation between total N removal and the total biomass, total N and the sum of the root tips, and total N and the sum of the root volume. This work shows the strong root-rock interactions effect on N that is characteristic of oligotrophic environments. Significant differences in total N between rock types

Micro-Computed Tomography Analysis of the Root Canal Morphology of Palatal Roots of Maxillary First Molars.

PubMed

Marceliano-Alves, Marília; Alves, Flávio Rodrigues Ferreira; Mendes, Daniel de Melo; Provenzano, José Claudio

2016-02-01

A thorough knowledge of root canal anatomy is critical for successful root canal treatments. This study evaluated the internal anatomy of the palatal roots of maxillary first molars with micro-computed tomography (microCT). The palatal roots of extracted maxillary first molars (n = 169) were scanned with microCT to determine several anatomic parameters, including main canal classification, lateral canal occurrence and location, degree of curvature, main foramen position, apical constriction presence, diameters 1 and 2 mm from the apex and 1 mm from the foramen, minor dentin thickness in those regions, canal volume, surface area, and convexity. All canals were classified as Vertucci type I. The cross sections were oval in 61% of the canals. Lateral canals were found in 25% of the samples. The main foramen did not coincide with the root apex in 95% of the cases. Only 8% of the canals were classified as straight. Apical constriction was identified in 38% of the roots. The minor and major canal diameters and minor dentin thickness were decreased near the apex. The minor dentin thickness 1 mm from the foramen was 0.82 mm. The palatal canals exhibited a volume of 6.91 mm(3) and surface area of 55.31 mm(2) and were rod-shaped. The root canals of the palatal roots were classified as type I. However, some factors need to be considered during the treatment of these roots, including the frequent ocurrence of moderate/severe curvatures, oval-shaped cross-sections, and lateral canals, noncoincidence of the apical foramen with the root apex, and absence of apical constriction in most cases. Copyright © 2016 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Impact of adhesive surface and volume of luting resin on fracture resistance of root filled teeth.

PubMed

Krastl, G; Gugger, J; Deyhle, H; Zitzmann, N U; Weiger, R; Müller, B

2011-05-01

To investigate the correlation between geometric parameters of severely compromised root filled (RCT) pre-molar teeth with irregular root canals and their fracture resistance. The null hypothesis tested was that the fracture resistance of root filled teeth is not influenced by: (i) the adhesive surface of the post-space preparation (A(PS) ), (ii) the coronal tooth surface (A(A) ), (iii) the amount of resin cement (V(C) ) and (iv) the Young's modulus of the specimens. A total of 48 noncarious human pre-molar teeth with irregular root canals were decoronated, root filled and adhesively restored with post-retained direct composite crowns. After thermomechanical loading (1,200,000×, 5-50° C), static load was applied until failure. The geometric parameters of the tooth were evaluated by microcomputed tomography (μCT) using impressions taken after post-space preparation. Linear regression analyses were performed to correlate the geometric parameters of the specimens with their fracture resistance. The amount of resin cement (V(C) ) comprised up to 88% of the entire post-space (mean 67%) and had no impact on the maximal load (P = 0.88). The latter was significantly influenced by post-space preparation (P = 0.003). Amongst the geometric parameters tested, the surface area in the root canal had the greatest impact on fracture resistance of root filled pre-molars restored with posts and composite crowns, whilst the fit of the post was less important. © 2011 International Endodontic Journal.

The paradoxical distribution of a shallow-rooted keystone species away from surface water, near the water-limited edge of its range in the Sonoran Desert: Seed-seedling conflicts

NASA Astrophysics Data System (ADS)

Drezner, Taly Dawn

2013-02-01

Species distributions reflect limiting factors, particularly near the margins of their range where density and abundance decrease as environmental factors decrease or increase to non-optimal conditions. I test whether the keystone saguaro cactus (Carnegiea gigantea), a shallow-rooted species, is indeed distributed disproportionately in areas of concentrated drainage (runnels) in a water-limited population. Carnegiea and a common nurse were sampled at a marginal site in and out of areas with concentrated surface water and chi-square analysis was used to determine the pattern of distribution. In this study I found that, surprisingly, near the hot, water-limited edge of their range, C. gigantea are found significantly less often in areas where more water would be available to them. For example, while only 20% of nurses were on interfluves, half of Carnegiea protégé were there. One possible explanation is that the subsequent redistribution of seeds by water away from preferred microsites may be important in shaping the final pattern of successful establishment. The shallow-rooted Carnegiea relies entirely on surface water for its moisture; it is thus paradoxical that the surface water so fundamentally essential to its survival throughout its life appears to hinder its establishment in precisely those sites where the greatest surface water would be available, particularly near the water-limited edge of its range.

An evolutionary attractor model for sapwood cross section in relation to leaf area.

PubMed

Westoby, Mark; Cornwell, William K; Falster, Daniel S

2012-06-21

Sapwood cross-sectional area per unit leaf area (SA:LA) is an influential trait that plants coordinate with physical environment and with other traits. We develop theory for SA:LA and also for root surface area per leaf area (RA:LA) on the premise that plants maximizing the surplus of revenue over costs should have competitive advantage. SA:LA is predicted to increase in water-relations environments that reduce photosynthetic revenue, including low soil water potential, high water vapor pressure deficit (VPD), and low atmospheric CO(2). Because sapwood has costs, SA:LA adjustment does not completely offset difficult water relations. Where sapwood costs are large, as in tall plants, optimal SA:LA may actually decline with (say) high VPD. Large soil-to-root resistance caps the benefits that can be obtained from increasing SA:LA. Where a plant can adjust water-absorbing surface area of root per leaf area (RA:LA) as well as SA:LA, optimal RA:SA is not affected by VPD, CO(2) or plant height. If selection favours increased height more so than increased revenue-minus-cost, then height is predicted to rise substantially under improved water-relations environments such as high-CO(2) atmospheres. Evolutionary-attractor theory for SA:LA and RA:LA complements models that take whole-plant conductivity per leaf area as a parameter. Copyright © 2012 Elsevier Ltd. All rights reserved.

Calibration of the Root Zone Water Quality Model and Application of Data Assimilation Techniques to Estimate Profile Soil Moisture

USDA-ARS?s Scientific Manuscript database

Estimation of soil moisture has received considerable attention in the areas of hydrology, agriculture, meteorology and environmental studies because of its role in the partitioning water and energy at the land surface. In this study, the USDA, Agricultural Research Service, Root Zone Water Quality ...

Aquatic Plants and Wastewater Treatment (an Overview)

NASA Technical Reports Server (NTRS)

Wolverton, B. C.

1986-01-01

The technology for using water hyacinth to upgrade domestic sewage effluent from lagoons and other wastewater treatment facilities to secondary and advanced secondary standards has been sufficiently developed to be used where the climate is warm year round. The technology of using emergent plants such as bulrush combined with duckweed is also sufficiently developed to make this a viable wastewater treatment alternative. This system is suited for both temperate and semi-tropical areas found throughout most of the U.S. The newest technology in artificial marsh wastewater treatment involves the use of emergent plant roots in conjunction with high surface area rock filters. Smaller land areas are required for these systems because of the increased concentration of microorganisms associated with the rock and plant root surfaces. Approximately 75 percent less land area is required for the plant-rock system than is required for a strict artificial wetland to achieve the same level of treatment.

Micropore surface area of alkali-soluble plant macromolecules (humic acids) drives their decomposition rates in soil.

PubMed

Papa, Gabriella; Spagnol, Manuela; Tambone, Fulvia; Pilu, Roberto; Scaglia, Barbara; Adani, Fabrizio

2010-02-01

Previous studies suggested that micropore surface area (MSA) of alkali-soluble bio-macromolecules of aerial plant residues of maize constitutes an important factor that explains their humification in soil, that is, preservation against biological degradation. On the other hand, root plant residue contributes to the soil humus balance, as well. Following the experimental design used in a previous paper published in this journal, this study shows that the biochemical recalcitrance of the alkali-soluble acid-insoluble fraction of the root plant material, contributed to the root maize humification of both Wild-type maize plants and its corresponding mutant brown midrib (bm3), this latter characterized by reduced lignin content. Humic acids (HAs) existed in root (root-HAs) were less degraded in soil than corresponding HAs existed in shoot (shoot-HAs): shoot-HAs bm3 (48%)>shoot-HAs Wild-type (37%)>root-HAs Wild-type (33%)>root-HAs bm3 (22%) (degradability shown in parenthesis). These differences were related to the MSA of HAs, that is, root-HAs having a higher MSA than shoot-HAs: shoot-HAs bm3 (41.43+/-1.2m(2)g(-1))

Drought resistance of Ailanthus altissima: root hydraulics and water relations.

PubMed

Trifilò, P; Raimondo, F; Nardini, A; Lo Gullo, M A; Salleo, S

2004-01-01

Drought resistance of Ailanthus altissima (Mill.) Swingle is a major factor underlying the impressively wide expansion of this species in Europe and North America. We studied the specific mechanism used by A. altissima to withstand drought by subjecting potted seedlings to four irrigation regimes. At the end of the 13-week treatment period, soil water potential was -0.05 MPa for well-watered control seedlings (W) and -0.4, -0.8 and -1.7 MPa for drought-stressed seedlings (S) in irrigation regimes S1, S2 and S3, respectively. Root and shoot biomass production did not differ significantly among the four groups. A progressively marked stomatal closure was observed in drought-stressed seedlings, leading to homeostasis of leaf water potential, which was maintained well above the turgor loss point. Root and shoot hydraulics were measured with a high-pressure flow meter. When scaled by leaf surface area, shoot hydraulic conductance did not differ among the treated seedlings, whereas root hydraulic conductance decreased by about 20% in S1 and S2 seedlings and by about 70% in S3 seedlings, with respect to the well-watered control value. Similar differences were observed when root hydraulic conductance was scaled by root surface area, suggesting that roots had become less permeable to water. Anatomical observations of root cross sections revealed that S3 seedlings had shrunken cortical cells and a multilayer endodermal-like tissue that probably impaired soil-to-root stele water transport. We conclude that A. altissima seedlings are able to withstand drought by employing a highly effective water-saving mechanism that involves reduced water loss by leaves and reduced root hydraulic conductance. This water-saving mechanism helps explain how A. altissima successfully competes with native vegetation.

The relationship between root hydraulics and scion vigour across Vitis rootstocks: what role do root aquaporins play?

PubMed Central

McElrone, A. J.

2012-01-01

Vitis vinifera scions are commonly grafted onto rootstocks of other grape species to influence scion vigour and provide resistance to soil-borne pests and abiotic stress; however, the mechanisms by which rootstocks affect scion physiology remain unknown. This study characterized the hydraulic physiology of Vitis rootstocks that vary in vigour classification by investigating aquaporin (VvPIP) gene expression, fine-root hydraulic conductivity (Lp r), % aquaporin contribution to Lp r, scion transpiration, and the size of root systems. Expression of several VvPIP genes was consistently greater in higher-vigour rootstocks under favourable growing conditions in a variety of media and in root tips compared to mature fine roots. Similar to VvPIP expression patterns, fine-root Lp r and % aquaporin contribution to Lp r determined under both osmotic (Lp r Osm) and hydrostatic (Lp r Hyd) pressure gradients were consistently greater in high-vigour rootstocks. Interestingly, the % aquaporin contribution was nearly identical for Lp r Osm and Lp r Hyd even though a hydrostatic gradient would induce a predominant flow across the apoplastic pathway. In common scion greenhouse experiments, leaf area-specific transpiration (E) and total leaf area increased with rootstock vigour and were positively correlated with fine-root Lp r. These results suggest that increased canopy water demands for scion grafted onto high-vigour rootstocks are matched by adjustments in root-system hydraulic conductivity through the combination of fine-root Lp r and increased root surface area. PMID:23136166

Quantitative imaging of radial oxygen loss from Valisneria spiralis roots with a fluorescent planar optode.

PubMed

Han, Chao; Ren, Jinghua; Tang, Hao; Xu, Di; Xie, Xianchuan

2016-11-01

Oxygen (O2) availability within the sediment-root interface is critical to the survival of macrophytes in O2-deficient sediment; however, our knowledge of the fine-scale impact of macrophyte roots upon the spatiotemporal dynamics of O2 is relatively limited. In this study, a non-invasive imaging technology was utilized to map O2 micro-distribution around Vallisneria spiralis. Long-term imaging results gathered during a 36day-period revealed an abundance of O2 spatiotemporal patterns ranging from 0 to 250μmolL(-1). The root-induced O2 leakage and consequent oxygenated area were stronger in the vicinity of the basal root compared to that found in the root tip. The O2 images revealed V. spiralis exhibited radial O2 loss (ROL) along the entire root, and the O2 distribution along the root length showed a high degree of small-scale spatial heterogeneity decreasing from 80% at the basal root surface to 10% at the root tip. The oxygenated zone area around the roots increased as O2 levels increased with root growth and irradiance intensities ranging from 0 to 216μmol photons m(-2)s(-1). A weak ROL measuring <20% air saturation around the basal root surface was maintained in darkness, which was presumably attributed to the O2 supply from overlying water via plant aerenchyma. The estimated total O2 release to the rhizosphere of V. spiralis was determined to range from 8.80±7.32 to 30.34±17.71nmolm(-2)s(-1), which is much higher than many other macrophyte species. This O2 release may be an important contribution to the high-capacity of V. spiralis for quickly colonizing anaerobic sediment. Copyright © 2016 Elsevier B.V. All rights reserved.

Ancestral QTL Alleles from Wild Emmer Wheat Enhance Root Development under Drought in Modern Wheat.

PubMed

Merchuk-Ovnat, Lianne; Fahima, Tzion; Ephrath, Jhonathan E; Krugman, Tamar; Saranga, Yehoshua

2017-01-01

A near-isogenic line (NIL-7A-B-2), introgressed with a quantitative trait locus (QTL) on chromosome 7AS from wild emmer wheat ( Triticum turgidum ssp. dicoccoides ) into the background of bread wheat ( T. aestivum L.) cv. BarNir, was recently developed and studied in our lab. NIL-7A-B-2 exhibited better productivity and photosynthetic capacity than its recurrent parent across a range of environments. Here we tested the hypothesis that root-system modifications play a major role in NIL-7A-B-2's agronomical superiority. Root-system architecture (dry matter and projected surface area) and shoot parameters of NIL-7A-B-2 and 'BarNir' were evaluated at 40, 62, and 82 days after planting (DAP) in a sand-tube experiment, and root tip number was assessed in a 'cigar-roll' seedling experiment, both under well-watered and water-limited (WL) treatments. At 82 DAP, under WL treatment, NIL-7A-B-2 presented greater investment in deep roots (depth 40-100 cm) than 'BarNir,' with the most pronounced effect recorded in the 60-80 cm soil depth (60 and 40% increase for root dry matter and surface area, respectively). NIL-7A-B-2 had significantly higher root-tip numbers (∼48%) per plant than 'BarNir' under both treatments. These results suggest that the introgression of 7AS QTL from wild emmer wheat induced a deeper root system under progressive water stress, which may enhance abiotic stress resistance and productivity of domesticated wheat.

Echocardiographic assessment of the aortic root dilatation in adult patients after tetralogy of Fallot repair.

PubMed

Cruz, Cristina; Pinho, Teresa; Lebreiro, Ana; Silva Cardoso, José; Maciel, Maria Júlia

2013-06-01

Transthoracic echocardiography is an important tool after tetralogy of Fallot repair, of which aortic root dilatation is a recognized complication. In this study we aimed to assess its prevalence and potential predictors. We consecutively assessed adult patients by transthoracic echocardiography after tetralogy of Fallot repair, and divided them into two groups based on the maximum internal aortic diameter at the sinuses of Valsalva in parasternal long-axis view: group 1 with aortic root dilatation (≥38 mm) and group 2 without dilatation (<38 mm). A total of 53 patients were included, mean age 32±10 years, with a mean time since surgery of 23±7 years. An aortopulmonary shunt had been performed prior to complete repair in 25 patients, and a transannular patch was used in 19 patients. Aortic root measurement was possible in all patients. Aortic root dilatation was identified in eight patients (15%), all male. Male gender (p=0.001), body surface area (1.93±0.10 vs. 1.70±0.20 m(2), p=0.03) and increased left ventricular end-diastolic diameter (p=0.005) were predictors of aortic root dilatation. None of the surgical variables studied were predictors of aortic root dilatation. The prevalence of aortic root dilatation in this cohort was low and male gender was a predictor of its occurrence. The type of repair and time to surgery did not influence its occurrence. Quantification of aortic root diameter is possible by transthoracic echocardiography; we suggest indexing it to body surface area in clinical practice. Copyright © 2012 Sociedade Portuguesa de Cardiologia. Published by Elsevier España. All rights reserved.

Duplicate and Conquer: Multiple Homologs of PHOSPHORUS-STARVATION TOLERANCE1 Enhance Phosphorus Acquisition and Sorghum Performance on Low-Phosphorus Soils1[C][W][OPEN

PubMed Central

Hufnagel, Barbara; de Sousa, Sylvia M.; Assis, Lidianne; Guimaraes, Claudia T.; Leiser, Willmar; Azevedo, Gabriel C.; Negri, Barbara; Larson, Brandon G.; Shaff, Jon E.; Pastina, Maria Marta; Barros, Beatriz A.; Weltzien, Eva; Rattunde, Henry Frederick W.; Viana, Joao H.; Clark, Randy T.; Falcão, Alexandre; Gazaffi, Rodrigo; Garcia, Antonio Augusto F.; Schaffert, Robert E.; Kochian, Leon V.; Magalhaes, Jurandir V.

2014-01-01

Low soil phosphorus (P) availability is a major constraint for crop production in tropical regions. The rice (Oryza sativa) protein kinase, PHOSPHORUS-STARVATION TOLERANCE1 (OsPSTOL1), was previously shown to enhance P acquisition and grain yield in rice under P deficiency. We investigated the role of homologs of OsPSTOL1 in sorghum (Sorghum bicolor) performance under low P. Association mapping was undertaken in two sorghum association panels phenotyped for P uptake, root system morphology and architecture in hydroponics and grain yield and biomass accumulation under low-P conditions, in Brazil and/or in Mali. Root length and root surface area were positively correlated with grain yield under low P in the soil, emphasizing the importance of P acquisition efficiency in sorghum adaptation to low-P availability. SbPSTOL1 alleles reducing root diameter were associated with enhanced P uptake under low P in hydroponics, whereas Sb03g006765 and Sb03g0031680 alleles increasing root surface area also increased grain yield in a low-P soil. SbPSTOL1 genes colocalized with quantitative trait loci for traits underlying root morphology and dry weight accumulation under low P via linkage mapping. Consistent allelic effects for enhanced sorghum performance under low P between association panels, including enhanced grain yield under low P in the soil in Brazil, point toward a relatively stable role for Sb03g006765 across genetic backgrounds and environmental conditions. This study indicates that multiple SbPSTOL1 genes have a more general role in the root system, not only enhancing root morphology traits but also changing root system architecture, which leads to grain yield gain under low-P availability in the soil. PMID:25189534

Fine root dynamics in lodgepole pine and white spruce stands along productivity gradients in reclaimed oil sands sites.

PubMed

Jamro, Ghulam Murtaza; Chang, Scott X; Naeth, M Anne; Duan, Min; House, Jason

2015-10-01

Open-pit mining activities in the oil sands region of Alberta, Canada, create disturbed lands that, by law, must be reclaimed to a land capability equivalent to that existed before the disturbance. Re-establishment of forest cover will be affected by the production and turnover rate of fine roots. However, the relationship between fine root dynamics and tree growth has not been studied in reclaimed oil sands sites. Fine root properties (root length density, mean surface area, total root biomass, and rates of root production, turnover, and decomposition) were assessed from May to October 2011 and 2012 using sequential coring and ingrowth core methods in lodgepole pine (Pinus contorta Dougl.) and white spruce (Picea glauca (Moench.) Voss) stands. The pine and spruce stands were planted on peat mineral soil mix placed over tailings sand and overburden substrates, respectively, in reclaimed oil sands sites in Alberta. We selected stands that form a productivity gradient (low, medium, and high productivities) of each tree species based on differences in tree height and diameter at breast height (DBH) increments. In lodgepole pine stands, fine root length density and fine root production, and turnover rates were in the order of high > medium > low productivity sites and were positively correlated with tree height and DBH and negatively correlated with soil salinity (P < 0.05). In white spruce stands, fine root surface area was the only parameter that increased along the productivity gradient and was negatively correlated with soil compaction. In conclusion, fine root dynamics along the stand productivity gradients were closely linked to stand productivity and were affected by limiting soil properties related to the specific substrate used for reconstructing the reclaimed soil. Understanding the impact of soil properties on fine root dynamics and overall stand productivity will help improve land reclamation outcomes.

Immunohistochemical study of bone sialoprotein and osteopontin in healthy and diseased root surfaces.

PubMed

Lao, Martin; Marino, Victor; Bartold, P Mark

2006-10-01

Periodontal disease is marked by inflammation and damage to tooth-supporting tissues. In particular, damage occurs to factors present in cementum that are thought to have the ability to influence the regeneration of surrounding tissues. Bone sialoprotein and osteopontin are major non-collagenous proteins in mineralized connective tissues associated with precementoblast chemo-attraction, adhesion to the root surface, and cell differentiation. The purpose of this investigation was to determine whether the expression and distribution of bone sialoprotein and osteopontin on root surfaces affected by periodontitis are altered compared to healthy, non-diseased root surfaces. Thirty healthy and 30 periodontitis-affected teeth were collected. Following fixation and demineralization, specimens were embedded in paraffin, sectioned, and exposed to antibodies against bone sialoprotein and osteopontin. Stained sections were assessed using light microscopy. Bone sialoprotein was not detected in the exposed cementum (absence of overlying periodontal ligament) of diseased teeth. In most areas where the periodontal ligament was intact, bone sialoprotein was detected for healthy and diseased teeth. For teeth reactive for bone sialoprotein, the matrix of the cementum just below the periodontal ligament was moderately stained. A similar immunoreactivity pattern for osteopontin was observed. The absence of bone sialoprotein and osteopontin staining along exposed cementum surfaces may be due to structural and compositional changes in matrix components associated with periodontal disease. This may influence the ability for regeneration and new connective tissue attachment onto previously diseased root surfaces.

Effectiveness of DIAGNOdent in Detecting Root Caries Without Dental Scaling Among Community-dwelling Elderly.

PubMed

Zhang, Wen; McGrath, Colman; Lo, Edward C M

The purpose of this clinical research was to analyze the effectiveness of DIAGNOdent in detecting root caries without dental scaling. The status of 750 exposed, unfilled root surfaces was assessed by visual-tactile examination and DIAGNOdent before and after root scaling. The sensitivity and specificity of different cut-off DIAGNOdent values in diagnosing root caries with reference to visual-tactile criteria were evaluated on those root surfaces without visible plaque/calculus. The DIAGNOdent values from sound and carious root surfaces were compared using the nonparametric Mann-Whitney U-test. The level of statistical significance was set at 0.05. On root surfaces without plaque/calculus, significantly different (p < 0.05) DIAGNOdent readings were obtained from sound root surfaces (12.2 ± 11.1), active carious root surfaces (37.6 ± 31.7) and inactive carious root surfaces (20.9 ± 10.5) before scaling. On root surfaces with visible plaque, DIAGNOdent readings obtained from active carious root surfaces (29.6 ± 20.8) and inactive carious root surfaces (27.0 ± 7.2) were not statistically significantly different (p > 0.05). Furthermore, on root surfaces with visible calculus, all DIAGNOdent readings obtained from sound root surfaces were > 50, which might be misinterpreted as carious. After scaling, the DIAGNOdent readings obtained from sound root surfaces (8.1 ± 11.3), active carious root surfaces (37.9 ± 31.9) and inactive carious root surfaces (24.9 ± 11.5) presented significant differences (p < 0.05). A cut-off value between 10 and 15 yielded the highest combined sensitivity and specificity in detecting root caries on root surfaces without visible plaque/calculus before scaling, but the combined sensitivity and specificity are both around 70%. These findings suggest that on exposed, unfilled root surfaces without visible plaque/calculus, DIAGNOdent can be used as an adjunct to the visual-tactile criteria in detecting root-surface status without pre-treatment by dental scaling.

Adaptive shoot and root responses collectively enhance growth at optimum temperature and limited phosphorus supply of three herbaceous legume species.

PubMed

Suriyagoda, Lalith D B; Ryan, Megan H; Renton, Michael; Lambers, Hans

2012-10-01

Studies on the effects of sub- and/or supraoptimal temperatures on growth and phosphorus (P) nutrition of perennial herbaceous species at growth-limiting P availability are few, and the impacts of temperature on rhizosphere carboxylate dynamics are not known for any species. The effect of three day/night temperature regimes (low, 20/13 °C; medium, 27/20 °C; and high, 32/25 °C) on growth and P nutrition of Cullen cinereum, Kennedia nigricans and Lotus australis was determined. The highest temperature was optimal for growth of C. cinereum, while the lowest temperature was optimal for K. nigricans and L. australis. At optimum temperatures, the relative growth rate (RGR), root length, root length per leaf area, total P content, P productivity and water-use efficiency were higher for all species, and rhizosphere carboxylate content was higher for K. nigricans and L. australis. Cullen cinereum, with a slower RGR, had long (higher root length per leaf area) and thin roots to enhance P uptake by exploring a greater volume of soil at its optimum temperature, while K. nigricans and L. australis, with faster RGRs, had only long roots (higher root length per leaf area) as a morphological adaptation, but had a higher content of carboxylates in their rhizospheres at the optimum temperature. Irrespective of the species, the amount of P taken up by a plant was mainly determined by root length, rather than by P uptake rate per unit root surface area. Phosphorus productivity was correlated with RGR and plant biomass. All three species exhibited adaptive shoot and root traits to enhance growth at their optimum temperatures at growth-limiting P supply. The species with a slower RGR (i.e. C. cinereum) showed only morphological root adaptations, while K. nigricans and L. australis, with faster RGRs, had both morphological and physiological (i.e. root carboxylate dynamics) root adaptations.

In vitro and clinical evaluation of optical coherence tomography for the detection of subgingival calculus and root cementum.

PubMed

Tsubokawa, Masaki; Aoki, Akira; Kakizaki, Sho; Taniguchi, Yoichi; Ejiri, Kenichiro; Mizutani, Koji; Koshy, Geena; Akizuki, Tatsuya; Oda, Shigeru; Sumi, Yasunori; Izumi, Yuichi

2018-05-24

This study evaluated the effectiveness of swept-source optical coherence tomography (ss-OCT) for detecting calculus and root cementum during periodontal therapy. Optical coherence tomography (OCT) images were taken before and after removal of subgingival calculus from extracted teeth and compared with non-decalcified histological sections. Porcine gingival sheets of various thicknesses were applied to the root surfaces of extracted teeth with calculus and OCT images were taken. OCT images were also taken before and after scaling and root planing (SRP) in human patients. In vitro, calculus was clearly detected as a white-gray amorphous structure on the root surface, which disappeared after removal. Cementum was identified as a thin, dark-gray layer. The calculus could not be clearly observed when soft tissues were present on the root surface. Clinically, supragingival calculus and cementum could be detected clearly with OCT, and subgingival calculus in the buccal cervical area of the anterior and premolar teeth was identified, which disappeared after SRP. Digital processing of the original OCT images was useful for clarifying the calculus. In conclusion, ss-OCT showed potential as a periodontal diagnostic tool for detecting cementum and subgingival calculus, although the practical applications of subgingival imaging remain limited.

Sulphate reduction and nitrogen fixation rates associated with roots, rhizomes and sediments from Zostera noltii and Spartina maritima meadows.

PubMed

Nielsen, L B; Finster, K; Welsh, D T; Donelly, A; Herbert, R A; de Wit, R; Lomstein, B A

2001-01-01

Sulphate reduction rates (SRR) and nitrogen fixation rates (NFR) associated with isolated roots, rhizomes and sediment from the rhizosphere of the marine macrophytes Zostera noltii and Spartina maritima, and the presence and distribution of Bacteria on the roots and rhizomes, were investigated. Between 1% and 3% of the surface area of the roots and rhizomes of both macrophytes were colonized by Bacteria. Bacteria on the surfaces of S. maritima roots and rhizomes were evenly distributed, while the distribution of Bacteria on Z. noltii roots and rhizomes was patchy. Root- and rhizome-associated SRR and NFR were always higher than rates in the bulk sediment. In particular, nitrogen fixation associated with the roots and rhizomes was 41-650-fold higher than in the bulk sediment. Despite the fact that sulphate reduction was elevated on roots and rhizomes compared with bulk sediment, the contribution of plant-associated sulphate reduction to overall sulphate reduction was small (< or =11%). In contrast, nitrogen fixation associated with the roots and rhizomes accounted for 31% and 91% of the nitrogen fixed in the rhizosphere of Z. noltii and S. maritima respectively. In addition, plant-associated nitrogen fixation could supply 37-1,613% of the nitrogen needed by the sulphate-reducing community. Sucrose stimulated nitrogen fixation and sulphate reduction significantly in the root and rhizome compartments of both macrophytes, but not in the bulk sediment.

Management of gingival recession by the use of an acellular dermal graft material: a 12-case series.

PubMed

Santos, A; Goumenos, G; Pascual, A

2005-11-01

Different soft tissue defects can be treated by a variety of surgical procedures. Most of these techniques require the palatal area as a donor site. Recently, an acellular dermal graft has become available that can substitute for palatal donor tissue. This study describes the surgical technique for gingival augmentation and root coverage and the results of 12 clinical cases. A comparison between the three most popular mucogingival procedures for root coverage is also presented. The results of the 12 patients and the 26 denuded surfaces have shown that we can obtain a mean root coverage of 74% with the acellular dermal graft. Thirteen out of the 26 denuded surfaces had complete root coverage. The average increase in keratinized tissue was 1.19 mm. It seems that the long-term results of the cases are stable. The proposed technique of root coverage with an acellular dermal graft can be a good alternative to soft tissue grafts for root coverage, and it should be part of our periodontal plastic surgery armamentarium.

A method for simulating transient ground-water recharge in deep water-table settings in central Florida by using a simple water-balance/transfer-function model

USGS Publications Warehouse

O'Reilly, Andrew M.

2004-01-01

A relatively simple method is needed that provides estimates of transient ground-water recharge in deep water-table settings that can be incorporated into other hydrologic models. Deep water-table settings are areas where the water table is below the reach of plant roots and virtually all water that is not lost to surface runoff, evaporation at land surface, or evapotranspiration in the root zone eventually becomes ground-water recharge. Areas in central Florida with a deep water table generally are high recharge areas; consequently, simulation of recharge in these areas is of particular interest to water-resource managers. Yet the complexities of meteorological variations and unsaturated flow processes make it difficult to estimate short-term recharge rates, thereby confounding calibration and predictive use of transient hydrologic models. A simple water-balance/transfer-function (WBTF) model was developed for simulating transient ground-water recharge in deep water-table settings. The WBTF model represents a one-dimensional column from the top of the vegetative canopy to the water table and consists of two components: (1) a water-balance module that simulates the water storage capacity of the vegetative canopy and root zone; and (2) a transfer-function module that simulates the traveltime of water as it percolates from the bottom of the root zone to the water table. Data requirements include two time series for the period of interest?precipitation (or precipitation minus surface runoff, if surface runoff is not negligible) and evapotranspiration?and values for five parameters that represent water storage capacity or soil-drainage characteristics. A limiting assumption of the WBTF model is that the percolation of water below the root zone is a linear process. That is, percolating water is assumed to have the same traveltime characteristics, experiencing the same delay and attenuation, as it moves through the unsaturated zone. This assumption is more accurate if the moisture content, and consequently the unsaturated hydraulic conductivity, below the root zone does not vary substantially with time. Results of the WBTF model were compared to those of the U.S. Geological Survey variably saturated flow model, VS2DT, and to field-based estimates of recharge to demonstrate the applicability of the WBTF model for a range of conditions relevant to deep water-table settings in central Florida. The WBTF model reproduced independently obtained estimates of recharge reasonably well for different soil types and water-table depths.

The Role of Smoking and Gingival Crevicular Fluid Markers on Coronally Advanced Flap Outcomes

PubMed Central

Kaval, Başak; Renaud, Diane E.; Scott, David A.; Buduneli, Nurcan

2015-01-01

Background This study evaluates possible effects of smoking on the following: 1) biochemical content in gingival crevicular fluid (GCF) samples from sites of gingival recession and saliva; and 2) clinical outcomes of coronally advanced flap (CAF) for root coverage. Methods Eighteen defects in 15 patients were included in each of the smoker and non-smoker groups. Baseline cotinine, basic fibroblast growth factor, vascular endothelial growth factor, platelet-derived growth factor, interleukin (IL)-8, IL-10, IL-12, tumor necrosis factor-α, matrix metalloproteinase (MMP)-8, MMP-9, and plasminogen activator inhibitor-1 levels were determined in GCF and saliva samples. CAF with microsurgery technique was applied. Plaque index, papilla bleeding index, recession depth (RD), recession width (RW), and root surface area were evaluated at baseline and postoperative months 1, 3, and 6. Probing depth, clinical attachment level (CAL), and keratinized gingival width (KGW) was recorded at baseline and month 6. Percentage of root coverage and complete root coverage were calculated at postoperative months 1, 3, and 6. Results All biochemical parameters were similar in the two groups apart from the definite difference in salivary cotinine concentrations (P = 0.000). Compared with the baseline values, RD, RW, CAL, and root surface area decreased, and KGW increased, with no significant difference between the study groups. CAL gain, percentage of root coverage, and complete root-coverage rates were similar in the study groups. Conclusion Similar baseline biochemical data and comparably high success rates of root coverage with CAF in systemically and periodontally healthy smokers versus non-smokers suggest lack of adverse effects of smoking on clinical outcomes. PMID:23725027

Intrapulpal temperatures during pulsed Nd:YAG laser treatment of dentin, in vitro.

PubMed

White, J M; Fagan, M C; Goodis, H E

1994-03-01

Lasers are being used for soft tissue removal, caries removal, and treatment of root surface sensitivity. One concern for laser safety is that the heat produced at the irradiated root surface may diffuse to the pulp causing irreversible pulpal damage. To test this heat diffusion, copper-constantan thermocouples were inserted into the radicular pulp canals of extracted teeth. Simulating direct exposure which might occur during gingival excision, superficial caries removal, and modification of the dentin surface for treatment of root surface sensitivity, a 2 mm2 area of the external root surface was uniformly irradiated with a pulsed Nd:YAG laser using a 320 microns diameter fiber optic contact probe. Power was varied from 0.3 to 3.0 W with frequencies of 10 and 20 Hz. Temperature changes during cavity preparations using a high speed handpiece with air coolant were also recorded. Repeated measures ANOVA (P < or = 0.05) indicated that intrapulpal temperatures increased as a function of power, frequency, and time. Intrapulpal temperatures decreased as remaining dentin thickness (0.2 to 2.0 mm) increased for each laser parameter. Irradiation of dentin using a Nd:YAG pulsed laser, within the treatment times, powers, and frequencies with adequate remaining dentin thickness, as outlined in this paper, should not cause devitalizing intrapulpal temperature rises.

Identifying the optimal spatially and temporally invariant root distribution for a semiarid environment

NASA Astrophysics Data System (ADS)

Sivandran, Gajan; Bras, Rafael L.

2012-12-01

In semiarid regions, the rooting strategies employed by vegetation can be critical to its survival. Arid regions are characterized by high variability in the arrival of rainfall, and species found in these areas have adapted mechanisms to ensure the capture of this scarce resource. Vegetation roots have strong control over this partitioning, and assuming a static root profile, predetermine the manner in which this partitioning is undertaken.A coupled, dynamic vegetation and hydrologic model, tRIBS + VEGGIE, was used to explore the role of vertical root distribution on hydrologic fluxes. Point-scale simulations were carried out using two spatially and temporally invariant rooting schemes: uniform: a one-parameter model and logistic: a two-parameter model. The simulations were forced with a stochastic climate generator calibrated to weather stations and rain gauges in the semiarid Walnut Gulch Experimental Watershed (WGEW) in Arizona. A series of simulations were undertaken exploring the parameter space of both rooting schemes and the optimal root distribution for the simulation, which was defined as the root distribution with the maximum mean transpiration over a 100-yr period, and this was identified. This optimal root profile was determined for five generic soil textures and two plant-functional types (PFTs) to illustrate the role of soil texture on the partitioning of moisture at the land surface. The simulation results illustrate the strong control soil texture has on the partitioning of rainfall and consequently the depth of the optimal rooting profile. High-conductivity soils resulted in the deepest optimal rooting profile with land surface moisture fluxes dominated by transpiration. As we move toward the lower conductivity end of the soil spectrum, a shallowing of the optimal rooting profile is observed and evaporation gradually becomes the dominate flux from the land surface. This study offers a methodology through which local plant, soil, and climate can be accounted for in the parameterization of rooting profiles in semiarid regions.

Evaluation of Teeth Development in Unilateral Cleft Lip and Palate Patients in Mixed Dentition by Using Medical Image Control Systems.

PubMed

Gezgin, O; Botsali, M S

2018-02-01

The aim of this study was to evaluate the crown and root development in patients with cleft lip and palate using medical software programmes. In our study, 25 patients with mixed dentition unilateral cleft lip and palate were examined with cone-beam computed tomography (CBCT). The tomography images obtained as high resolution medical images on the computer control system (MIMICS 15.0, Materialise, Leuven, Belgium and SOLIDWORKS 2014 Premium, Concord, Massachusetts) were converted to three-dimensional volumetric images. These three-dimensional images of the cleft on the sides of the teeth in the crown and root growth were measured by mesiodistal length and crown/root rate with volume and area. These measurements were compared with a control group of healthy individuals. There were no statistically significant differences in the volume, surface area and MD size, crown/root ratio of central incisor, canine, first premolar and second premolar teeth within defect, and healthy teeth. However, it was found that there was a significant difference between the volume, surface area and MD size, and crown/root ratio of the lateral teeth in each group. In particular, among patients with cleft lip and palate, on obtaining a solid model of the tooth structure by using these programs, tooth development can be examined in more detail, diagnosis can be made more reliable, as well as in treatment planning. We believe that these programs can be used to resolve certain limitations such as a lack of an application to be used in routine dental treatment and in particular the need to do more study.

Mercury net methylation in five tropical flood plain regions of Brazil: high in the root zone of floating macrophyte mats but low in surface sediments and flooded soils.

PubMed

Guimarães, J R; Meili, M; Hylander, L D; de Castro e Silva, E; Roulet, M; Mauro, J B; de Lemos, R

2000-10-16

In aquatic systems, bottom sediments have often been considered as the main methylmercury (MeHg) production site. In tropical floodplain areas, however, floating meadows and flooded forests extend over large areas and can be important Hg methylating sites. We present here a cross-system comparison of the Hg net methylation capacity in surface sediments, flooded soils and roots of floating aquatic macrophytes, assayed by in situ incubation with 203Hg and extraction of formed Me203 Hg by acid leaching and toluene. The presence of mono-MeHg was confirmed by thin layer chromatography and other techniques. Study areas included floodplain lakes in the Amazon basin (Tapajós, Negro and Amazon rivers), the Pantanal floodplain (Paraguay river basin), freshwater coastal lagoons in Rio de Janeiro and oxbow lakes in the Mogi-Guaçú river, São Paulo state. Different Hg levels were added in assays performed in 1994-1998, but great care was taken to standardise all other test parameters, to allow data comparisons. Net MeHg production was one order of magnitude higher (mean 13.8%, range 0.28-35) in the living or decomposing roots of floating or rooted macrophyte mats (Eichhornia azurea, E. crassipes, Paspalum sp., Eleocharis sellowiana, Salvinia sp., S. rotundifolia and Scirpus cubensis) than in the surface layer of underlying lake sediments (mean 0.6%, range 0.022-2.5). Methylation in flooded soils presented a wide range and was in some cases similar to the one found in macrophyte roots but usually much lower. In a Tapajós floodplain lake, natural concentrations of MeHg in soil and sediment cores taken along a lake-forest transect agreed well with data on net methylation potentials in the same samples. E. azurea, E. crassipes and Salvinia presented the highest methylation potentials, up to 113 times higher than in sediments. Methylation in E. azurea from six lakes of the Paraguay and Cuiabá rivers, high Pantanal, was determined in the 1998 dry and wet seasons and ranged from 1.8 to 35%. Methylation was lower in washed roots than in untreated roots of E. azurea and methylation in solids isolated from the roots, was higher than in sediments but lower than in untreated roots. This indicates that the methylation in roots zones occurs mainly in the root-associated solids. Floating meadows are sites of intense production of biomass and of highly bioavailable MeHg and appear to be an essential link of the MeHg cycle in tropical aquatic systems.

Asymmetrical development of root endodermis and exodermis in reaction to abiotic stresses

PubMed Central

Líška, Denis; Martinka, Michal; Kohanová, Jana; Lux, Alexander

2016-01-01

Background and Aims In the present study, we show that development of endodermis and exodermis is sensitively regulated by water accessibility. As cadmium (Cd) is known to induce xeromorphic effects in plants, maize roots were exposed also to Cd to understand the developmental process of suberin lamella deposition in response to a local Cd source. Methods In a first experiment, maize roots were cultivated in vitro and unilaterally exposed to water-containing medium from one side and to air from the other. In a second experiment, the roots were placed between two agar medium layers with a strip of Cd-containing medium attached locally and unilaterally to the root surface. Key Results The development of suberin lamella (the second stage of exodermal and endodermal development) started asymmetrically, preferentially closer to the root tip on the side exposed to the air. In the root contact with Cd in a spatially limited area exposed to one side of the root, suberin lamella was preferentially developed in the contact region and additionally along the whole length of the root basipetally from the contact area. However, the development was unilateral and asymmetrical, facing the treated side. The same pattern occurred irrespective of the distance of Cd application from the root apex. Conclusions These developmental characteristics indicate a sensitive response of root endodermis and exodermis in the protection of vascular tissues against abiotic stresses. PMID:27112163

Ammonium and Nitrate Uptake by the Floating Plant Landoltia punctata

PubMed Central

Fang, Yun Ying; Babourina, Olga; Rengel, Zed; Yang, Xiao E.; Pu, Pei Min

2007-01-01

Background and Aims Plants from the family Lemnaceae are widely used in ecological engineering projects to purify wastewater and eutrophic water bodies. However, the biology of nutrient uptake mechanisms in plants of this family is still poorly understood. There is controversy over whether Lemnaceae roots are involved in nutrient uptake. No information is available on nitrogen (N) preferences and capacity of Landoltia punctata (dotted duckweed), one of the best prospective species in Lemnaceae for phytomelioration and biomass production. The aim of this study was to assess L. punctata plants for their ability to take up NH4+ and NO3− by both roots and fronds. Methods NO3− and NH4+ fluxes were estimated by a non-invasive ion-selective microelectrode technique. This technique allows direct measurements of ion fluxes across the root or frond surface of an intact plant. Key Results Landoltia punctata plants took up NH4+ and NO3− by both fronds and roots. Spatial distribution of NH4+ and NO3− fluxes demonstrated that, although ion fluxes at the most distal parts of the root were uneven, the mature part of the root was involved in N uptake. Despite the absolute flux values for NH4+ and NO3− being lower in roots than at the frond surface, the overall capacity of roots to take up ions was similar to that of fronds because the surface area of roots was larger. L. punctata plants preferred to take up NH4+ over NO3− when both N sources were available. Conclusions Landoltia punctata plants take up nitrogen by both roots and fronds. When both sources of N are available, plants prefer to take up NH4+, but will take up NO3− when it is the only N source. PMID:17204539

Characterization of a rice variety with high hydraulic conductance and identification of the chromosome region responsible using chromosome segment substitution lines

PubMed Central

Adachi, Shunsuke; Tsuru, Yukiko; Kondo, Motohiko; Yamamoto, Toshio; Arai-Sanoh, Yumiko; Ando, Tsuyu; Ookawa, Taiichiro; Yano, Masahiro; Hirasawa, Tadashi

2010-01-01

Background and Aims The rate of photosynthesis in paddy rice often decreases at noon on sunny days because of water stress, even under submerged conditions. Maintenance of higher rates of photosynthesis during the day might improve both yield and dry matter production in paddy rice. A high-yielding indica variety, ‘Habataki’, maintains a high rate of leaf photosynthesis during the daytime because of the higher hydraulic conductance from roots to leaves than in the standard japonica variety ‘Sasanishiki’. This research was conducted to characterize the trait responsible for the higher hydraulic conductance in ‘Habataki’ and identified a chromosome region for the high hydraulic conductance. Methods Hydraulic conductance to passive water transport and to osmotic water transport was determined for plants under intense transpiration and for plants without transpiration, respectively. The varietal difference in hydraulic conductance was examined with respect to root surface area and hydraulic conductivity (hydraulic conductance per root surface area, Lp). To identify the chromosome region responsible for higher hydraulic conductance, chromosome segment substitution lines (CSSLs) derived from a cross between ‘Sasanishiki’ and ‘Habataki’ were used. Key Results The significantly higher hydraulic conductance resulted from the larger root surface area not from Lp in ‘Habataki’. A chromosome region associated with the elevated hydraulic conductance was detected between RM3916 and RM2431 on the long arm of chromosome 4. The CSSL, in which this region was substituted with the ‘Habataki’ chromosome segment in the ‘Sasanishiki’ background, had a larger root mass than ‘Sasanishiki’. Conclusions The trait for increasing plant hydraulic conductance and, therefore, maintaining the higher rate of leaf photosynthesis under the conditions of intense transpiration in ‘Habataki’ was identified, and it was estimated that there is at least one chromosome region for the trait located on chromosome 4. PMID:20810742

Reference Values of Aortic Root in Male and Female White Elite Athletes According to Sport.

PubMed

Boraita, Araceli; Heras, Maria-Eugenia; Morales, Francisco; Marina-Breysse, Manuel; Canda, Alicia; Rabadan, Manuel; Barriopedro, Maria-Isabel; Varela, Amai; de la Rosa, Alejandro; Tuñón, José

2016-10-01

There is limited information regarding the aortic root upper physiological limits in all planes in elite athletes according to static and dynamic cardiovascular demands and sex. A cross-sectional study was performed in 3281 healthy elite athletes (2039 men and 1242 women) aged 23.1±5.7 years, with body surface area of 1.9±0.2 m 2 and 8.9±4.9 years and 19.2±9.6 hours/week of training. Maximum end-diastolic aortic root diameters were measured in the parasternal long axis by 2-dimensional echocardiography. Age, left ventricular mass, and body surface area were the main predictors of aortic dimensions. Raw values were greater in males than in females (P<0.0001) at all aortic root levels. Dimensions corrected by body surface area were higher in men than in women at the aortic annulus (13.1±1.7 versus 12.9±1.7 mm/m 2 ; P=0.007), without significant differences at the sinus of Valsalva (16.3±1.9 versus 16.3±1.9 mm/m 2 ; P=0.797), and were smaller in men at the sinotubular junction (13.6±1.8 versus 13.8±1.8 mm/m 2 ; P=0.008) and the proximal ascending aorta (13.8±1.9 versus 14.1±1.9 mm/m 2 ; P=0.001). Only 1.8% of men and 1.5% of women had values >40 mm and 34 mm, respectively. Raw and corrected aortic measures at all levels were significantly greater in sports, with a high dynamic component in both sexes, except for corrected values of the sinotubular junction in women. Aortic root dimensions in healthy elite athletes are within the established limits for the general population. This study describes the normal dimensions for healthy elite athletes classified according to sex and dynamic and static components of their sports. © 2016 American Heart Association, Inc.

Analysis of the relationship between rusty root incidences and soil properties in Panax ginseng

NASA Astrophysics Data System (ADS)

Wang, Q. X.; Xu, C. L.; Sun, H.; Ma, L.; Li, L.; Zhang, D. D.; Zhang, Y. Y.

2016-08-01

Rusty root is a serious problem in ginseng cultivation that limits the production and quality of ginseng worldwide. The Changbai Mountains are the most famous area for ginseng cultivation in China. To clarify the relationship between rusty root and soil characteristics, physico-chemical properties and enzymatic activities of soil collected from five different fields in the Changbai Mountains were analyzed and a controlled experiment carried out by increasing the concentration of Fe (II). Soil bulk density, moisture, total iron (Fe) and total manganese (Mn) concentrations and polyphenol oxidase (PPO) activity were significantly higher in rusty root than healthy root groups (two-sample test, P<0.05 or P<0.01), respectively. Pearson test showed that there was a significant positive correlation between rusty root index and pH, N, Fe, Mn, Al, Zn and Ca of soil samples collected from fields (P<0.05 or P<0.01), and a significant positive correlation also occurred between rusty root index and Fe (II) added to soil in Fe (II) inducing rusty root (P<0.01). Physiological factors may be very important roles giving rise to ginseng rusty root. Fe (III) reduction and Fe (II) oxidation could be important in increasing the incidence of rusty root. Soil moisture and bulk density of non-rhizosphere soil not attached to the root surface, and pH, N and PPO content of rhizosphere soils attached to the root surface were heavily involved in the reduction, oxidation and sequestration of metal ions.

Clinical attachment level gain and bone regeneration around a glass ionomer restoration on root surface wall of periodontal pocket

PubMed Central

Biniraj, K. R.; Sagir, Mohammed; Sunil, M. M.; Janardhanan, Mahija

2012-01-01

A case describing perio-restorative management of an accidental trauma in the mid portion of root on an upper left canine tooth following an ostectomy surgery is presented here. The traumatized root area was undergoing fast resorption and a chronic periodontal abscess had developed in relation to the lesion. The article illustrates the clinical and radiographic photo series of a periodontal flap surgery done to gain access into a subgingival region for the placement of Glass ionomer restoration on the root and its periodic follow up. The clinical condition of the area suggests 8 mm clinical attachment gain over the restoration and the review radiographs at definite intervals up to 18 months revealed evidence of consistent bone regeneration around the restoration. The article also highlights the various other possibilities, where this restorative material can be effectively used in conjunction with periodontal surgical procedures. PMID:23162344

[The mechanism of root hair development and molecular regulation in plants].

PubMed

Wang, Yue-Ping; Li, Ying-Hui; Guan, Rong-Xia; Liu, Zhang-Xiong; Chen, Xiong-Ting; Chang, Ru-Zhen; Qiu, Li-Juan

2007-04-01

The formation of the root epidermis in Arabidopsis thaliana provides a simple model to study mechanisms underlying patterning in plants. Root hair increases the root surface area and effectively increases the root diameter, so root hair is thought to aid plants in nutrient uptake, anchorage and microbe interactions. The determination of root hair development has two types, lateral inhibition with feedback and position-dependent pattern of cell differentiation. The initiation and development of root hair in Arabidopsis provide a simple and efficacious model for the study of cell fate determination in plants. Molecular genetic studies identify a suite of putative transcription factors which regulate the epidermal cell pattern. The homeodomain protein GLABRA2 (GL2), R2R3 MYB-type transcription factor WEREWOLF (WER) and WD-repeat protein TRANSPARENTT TESTA GLABRA (TTG) are required for specification of non-hair cell type. The CAPRICE (CPC) and TRYPTICHON (TRY) are involved in specifying the hair cell fate.

The response of mulberry trees after seedling hardening to summer drought in the hydro-fluctuation belt of Three Gorges Reservoir Areas.

PubMed

Huang, Xiaohui; Liu, Yun; Li, Jiaxing; Xiong, Xingzheng; Chen, Yang; Yin, Xiaohua; Feng, Dalan

2013-10-01

Interest has developed in the potential of mulberry (Morus alba), a woody perennial, for revegetating the hydro-fluctuation belt of the Three Gorges Reservoir due to its resistance to water-logging stress. To be useful, the trees must also be able to withstand dry conditions in summer when temperatures can be very high and droughts become severe quickly. Here, we report a study in which mulberry seedlings were grown in a greenhouse under a variety of simulated soil water conditions reflecting potential summer scenarios in the hydro-fluctuation belt of the Three Gorges Reservoir Area. We compared the responses of two pretreatment groups of mulberry seedlings to different levels of drought stress. The pretreatment groups differed with respect to drought hardening: the daily-managed (DM) group had relative soil moisture held constant in the range 70-80 %, while the drought-hardened (DH) group had relative soil moisture held constant at 40-50 %. Following the month-long pretreatment of seedlings, the two groups of young trees (DM and DH) were then respectively subjected to three levels of drought stress for a month: normal watering, moderate drought stress, and severe drought stress. A series of measurements comparing the physiological status of the plants in the two groups were then made, and the following results were obtained: (1) As drought stress increased, the heights, base diameters, root surface areas, photosynthetic rates (Pn), stomatal conductances (Gs), and transpiration rates (Tr) of the mulberry trees in both groups (DM and DH) decreased significantly, while the specific root area and abscisic acid (ABA) contents had increasing trends. Root activity and instantaneous water use efficiency of mulberry trees in both groups (DM and DH) were all raised under drought stress conditions than under normal watering, but the root/shoot ratio and leaf water potential were lowered. (2) At the same level of soil water content, the heights, base diameters, root/shoot ratios, root surface areas, specific root areas, photosynthetic rates (Pn), stomatal conductances (Gs), and transpiration rates (Tr) of the young mulberry trees in the DH were all significantly higher than those of the control group (DM). Leaf water potential, instantaneous water use efficiency, and abscisic acid content of DH were all significantly lower than DM. Under different degrees of drought stress, the growth of mulberry trees will be inhibited, but the trees can respond to the stress by increasing the root absorptive area and enhancing capacity for water retention. Mulberry trees demonstrate strong resistance to drought stress, and furthermore drought resistance can be improved by drought hardening during the seedling stage.

Can the KTP laser change the cementum surface of healthy and diseased teeth providing an acceptable root surface for fibroblast attachment?

NASA Astrophysics Data System (ADS)

Mailhot, Jason M.; Garnick, Jerry J.

1996-04-01

The purpose of our research is to determine the effects of KTP laser on root cementum and fibroblast attachment. Initial work has been completed in testing the effect of different energy levels on root surfaces. From these studies optimal energy levels were determined. In subsequent studies the working distance and exposure time required to obtain significant fibroblast attachment to healthy cementum surfaces were investigated. Results showed that lased cemental surfaces exhibited changes in surface topography which ranged from a melted surface to an apparent slight fusion of the surface of the covering smear layer. When the optimal energy level was used, fibroblasts demonstrate attachment on the specimens, resulting in the presence of a monolayer of cells on the control surfaces as well as on the surfaces lased with this energy level. The present study investigates the treatment of pathological root surfaces and calculus with a KTP laser utilizing these optimal parameters determine previously. Thirty single rooted teeth with advanced periodontal disease and ten healthy teeth were obtained, crowns were sectioned and roots split longitudinally. Forty test specimens were assigned into 1 of 4 groups; pathologic root--not lased, pathologic root--lased, root planed root and health root planed root. Human gingival fibroblasts were seeded on specimens and cultured for 24 hours. Specimens were processed for SEM. The findings suggest that with the KTP laser using a predetermined energy level applied to pathological root surfaces, the lased surfaces provided an unacceptable surface for fibroblast attachment. However, the procedural control using healthy root planed surfaces did demonstrate fibroblast attachment.

Atenolol versus Losartan in Children and Young Adults with Marfan's Syndrome

PubMed Central

Lacro, R.V.; Dietz, H.C.; Sleeper, L.A.; Yetman, A.T.; Bradley, T.J.; Colan, S.D.; Pearson, G.D.; Tierney, E.S. Selamet; Levine, J.C.; Atz, A.M.; Benson, D.W.; Braverman, A.C.; Chen, S.; De Backer, J.; Gelb, B.D.; Grossfeld, P.D.; Klein, G.L.; Lai, W.W.; Liou, A.; Loeys, B.L.; Markham, L.W.; Olson, A.K.; Paridon, S.M.; Pemberton, V.L.; Pierpont, M.E.; Pyeritz, R.E.; Radojewski, E.; Roman, M.J.; Sharkey, A.M.; Stylianou, M.P.; Wechsler, S. Burns; Young, L.T.; Mahony, L.

2015-01-01

BACKGROUND Aortic-root dissection is the leading cause of death in Marfan's syndrome. Studies suggest that with regard to slowing aortic-root enlargement, losartan may be more effective than beta-blockers, the current standard therapy in most centers. METHODS We conducted a randomized trial comparing losartan with atenolol in children and young adults with Marfan's syndrome. The primary outcome was the rate of aortic-root enlargement, expressed as the change in the maximum aortic-root-diameter z score indexed to body-surface area (hereafter, aortic-root z score) over a 3-year period. Secondary outcomes included the rate of change in the absolute diameter of the aortic root; the rate of change in aortic regurgitation; the time to aortic dissection, aortic-root surgery, or death; somatic growth; and the incidence of adverse events. RESULTS From January 2007 through February 2011, a total of 21 clinical centers enrolled 608 participants, 6 months to 25 years of age (mean [±SD] age, 11.5±6.5 years in the atenolol group and 11.0±6.2 years in the losartan group), who had an aortic-root z score greater than 3.0. The baseline-adjusted rate of change (±SE) in the aortic-root z score did not differ significantly between the atenolol group and the losartan group (−0.139±0.013 and −0.107±0.013 standard-deviation units per year, respectively; P = 0.08). Both slopes were significantly less than zero, indicating a decrease in the degree of aortic-root dilatation relative to body-surface area with either treatment. The 3-year rates of aortic-root surgery, aortic dissection, death, and a composite of these events did not differ significantly between the two treatment groups. CONCLUSIONS Among children and young adults with Marfan's syndrome who were randomly assigned to losartan or atenolol, we found no significant difference in the rate of aortic-root dilatation between the two treatment groups over a 3-year period. (Funded by the National Heart, Lung, and Blood Institute and others; ClinicalTrials.gov number, NCT00429364.) PMID:25405392

Atenolol versus losartan in children and young adults with Marfan's syndrome.

PubMed

Lacro, Ronald V; Dietz, Harry C; Sleeper, Lynn A; Yetman, Anji T; Bradley, Timothy J; Colan, Steven D; Pearson, Gail D; Selamet Tierney, E Seda; Levine, Jami C; Atz, Andrew M; Benson, D Woodrow; Braverman, Alan C; Chen, Shan; De Backer, Julie; Gelb, Bruce D; Grossfeld, Paul D; Klein, Gloria L; Lai, Wyman W; Liou, Aimee; Loeys, Bart L; Markham, Larry W; Olson, Aaron K; Paridon, Stephen M; Pemberton, Victoria L; Pierpont, Mary Ella; Pyeritz, Reed E; Radojewski, Elizabeth; Roman, Mary J; Sharkey, Angela M; Stylianou, Mario P; Wechsler, Stephanie Burns; Young, Luciana T; Mahony, Lynn

2014-11-27

Aortic-root dissection is the leading cause of death in Marfan's syndrome. Studies suggest that with regard to slowing aortic-root enlargement, losartan may be more effective than beta-blockers, the current standard therapy in most centers. We conducted a randomized trial comparing losartan with atenolol in children and young adults with Marfan's syndrome. The primary outcome was the rate of aortic-root enlargement, expressed as the change in the maximum aortic-root-diameter z score indexed to body-surface area (hereafter, aortic-root z score) over a 3-year period. Secondary outcomes included the rate of change in the absolute diameter of the aortic root; the rate of change in aortic regurgitation; the time to aortic dissection, aortic-root surgery, or death; somatic growth; and the incidence of adverse events. From January 2007 through February 2011, a total of 21 clinical centers enrolled 608 participants, 6 months to 25 years of age (mean [±SD] age, 11.5±6.5 years in the atenolol group and 11.0±6.2 years in the losartan group), who had an aortic-root z score greater than 3.0. The baseline-adjusted rate of change in the mean (±SE) aortic-root z score did not differ significantly between the atenolol group and the losartan group (-0.139±0.013 and -0.107±0.013 standard-deviation units per year, respectively; P=0.08). Both slopes were significantly less than zero, indicating a decrease in the aortic-root diameter relative to body-surface area with either treatment. The 3-year rates of aortic-root surgery, aortic dissection, death, and a composite of these events did not differ significantly between the two treatment groups. Among children and young adults with Marfan's syndrome who were randomly assigned to losartan or atenolol, we found no significant difference in the rate of aortic-root dilatation between the two treatment groups over a 3-year period. (Funded by the National Heart, Lung, and Blood Institute and others; ClinicalTrials.gov number, NCT00429364.).

Plant-Sediment Interactions in Salt Marshes - An Optode Imaging Study of O2, pH, and CO 2 Gradients in the Rhizosphere.

PubMed

Koop-Jakobsen, Ketil; Mueller, Peter; Meier, Robert J; Liebsch, Gregor; Jensen, Kai

2018-01-01

In many wetland plants, belowground transport of O 2 via aerenchyma tissue and subsequent O 2 loss across root surfaces generates small oxic root zones at depth in the rhizosphere with important consequences for carbon and nutrient cycling. This study demonstrates how roots of the intertidal salt-marsh plant Spartina anglica affect not only O 2 , but also pH and CO 2 dynamics, resulting in distinct gradients of O 2 , pH, and CO 2 in the rhizosphere. A novel planar optode system (VisiSens TD ® , PreSens GmbH) was used for taking high-resolution 2D-images of the O 2 , pH, and CO 2 distribution around roots during alternating light-dark cycles. Belowground sediment oxygenation was detected in the immediate vicinity of the roots, resulting in oxic root zones with a 1.7 mm radius from the root surface. CO 2 accumulated around the roots, reaching a concentration up to threefold higher than the background concentration, and generally affected a larger area within a radius of 12.6 mm from the root surface. This contributed to a lowering of pH by 0.6 units around the roots. The O 2 , pH, and CO 2 distribution was recorded on the same individual roots over diurnal light cycles in order to investigate the interlinkage between sediment oxygenation and CO 2 and pH patterns. In the rhizosphere, oxic root zones showed higher oxygen concentrations during illumination of the aboveground biomass. In darkness, intraspecific differences were observed, where some plants maintained oxic root zones in darkness, while others did not. However, the temporal variation in sediment oxygenation was not reflected in the temporal variations of pH and CO 2 around the roots, which were unaffected by changing light conditions at all times. This demonstrates that plant-mediated sediment oxygenation fueling microbial decomposition and chemical oxidation has limited impact on the dynamics of pH and CO 2 in S. anglica rhizospheres, which may in turn be controlled by other processes such as root respiration and root exudation.

Periodontal ligament hydrostatic pressure with areas of root resorption after application of a continuous torque moment.

PubMed

Hohmann, Ansgar; Wolfram, Uwe; Geiger, Martin; Boryor, Andrew; Sander, Christian; Faltin, Rolf; Faltin, Kurt; Sander, Franz Guenter

2007-07-01

To evaluate the risk of root resorption, individual finite element models (FEMs) of extracted human maxillary first premolars were created, and the distribution of the hydrostatic pressure in the periodontal ligament (PDL) of these models was simulated. A continuous lingual torque of 3 Nmm and 6 Nmm respectively was applied in vivo to the aforementioned teeth. After extraction, FEMs of these double-rooted teeth were created based on high-resolution microcomputed tomographics (micro CT, voxel size: 35 microns). This high volumetric resolution made the recognition of very small resorption lacunae possible. Scanning electron micrographs of the root surfaces were created as well. This enabled the investigation of advantages and disadvantages of the different imaging techniques from the viewpoint of the examination of root resorption. Using the FEMs, the same loading conditions as applied in vivo were simulated. The results of clinical examination and simulations were compared using the identical roots of the teeth. The regions that showed increased hydrostatic pressure (>0.0047 MPa) correlated well with the locations of root resorption for each tooth. Increased torque resulted in increased high-pressure areas and increased magnitudes of hydrostatic pressure, correlating with the experiments. If hydrostatic pressure exceeds typical human capillary blood pressure in the PDL, the risk of root resorption increases.

Mind the Roots: Phenotyping Below-Ground Crop Diversity and Its Influence on Final Yield

NASA Astrophysics Data System (ADS)

Nieters, C.; Guadagno, C. R.; Lemli, S.; Hosseini, A.; Ewers, B. E.

2017-12-01

Changes in global climate patterns and water regimes are having profound impacts on worldwide crop production. An ever-growing population paired with increasing temperatures and unpredictable periods of severe drought call for accurate modeling of future crop yield. Although novel approaches are being developed in high-throughput, above-ground image phenotyping, the below-ground plant system is still poorly phenotyped. Collection of plant root morphology and hydraulics are needed to inform mathematical models to reliably estimate yields of crops grown in sub-optimal conditions. We used Brassica rapa to inform our model as it is a globally cultivated crop with several functionally diverse cultivars. Specifically, we use 7 different accessions from oilseed (R500 and Yellow Sarson), leafy type (Pac choi and Chinese cabbage), a vegetable turnip, and two Wisconsin Fast Plants (Imb211 and Fast Plant self-compatible), which have shorter life cycles and potentially large differences in allocation to roots. Bi-weekly, we harvested above and below-ground biomass to compare the varieties in terms of carbon allocation throughout their life cycle. Using WinRhizo software, we analyzed root system length and surface area to compare and contrast root morphology among cultivars. Our results confirm that root structural characteristics are crucial to explain plant water use and carbon allocation. The root:shoot ratio reveals a significant (p < 0.01) difference among crop accession. To validate the procedure across different varieties and life stages we also compared surface area results from the image-based technology to dry biomass finding a strong linear relationship (R2= 0.85). To assess the influence of a diverse above-ground morphology on the root system we also measured above-ground anatomical and physiological traits such as gas exchange, chlorophyll content, and chlorophyll a fluorescence. A thorough analysis of the root system will clarify carbon dynamics and hydraulics at the whole-plant level, improving final yield predictions.

[Root anatomical structure and hydraulic traits of three typical shrubs on the sandy lands of northern Shaanxi Province, China].

PubMed

Ai, Shao-shui; Li, Yang-yang; Chen, Jia-cun; Chen, Wei-yue

2015-11-01

Root xylem anatomical structure and hydraulic traits of three typical shrubs, i.e., Salix psammophila, Caragana korshinskii and Hippophae rhamnoides, within two soil layers (0-20 cm and 30-50 cm) were compared. The results showed that S. psammophila had a higher leaf water potential than C. korshinskii and H. rhamnoides, the average maximum and minimum lumen diameter (d(max) and d(min), respectively), the average lumen area of vessels (Alum) and the ratio of lumen area of all vessels to xylem area (Aves/Axyl) in S. psammophila roots were also significantly higher than those in C. korshinskii and H. rhamnoides, and the root vessel density (VD) in S. psammophila was the same as that in H. rhamnoides but significantly higher than that in C. korshinskii. Root hydraulic conductivity in S. psammophila was 5 times of C. korshinskii and 2.8 times of H. hamnoides. The vulnerability index in S. psammophila roots was similar to that in C. korshinskii but higher than that in H. hamnoides. S. psammophila belonged to a water-spending species, whereas both C. korshinskii and H. rhamnoides were water-saving species, and C. korshinskii was more drought-resistant than H. rhamnoides. There was no difference of d(max), d(min) and Alum between roots in two soil layers, but roots within in the 30-50 cm soil layer had larger VD and Aves/Axyl. The root specific hydraulic conductivity within the 30-50 cm soil layer was significantly higher than within the surface soil layer, whereas the vulnerability index within the 30-50 cm soil layer was smaller, indicating roots in deep soil layers had higher hydraulic transport efficiency and lower hydraulic vulnerability.

Root nodulation in the wetland tree Pterocarpus officinalis along coastal and montane systems of Northeast of Puerto Rico

Treesearch

Rachel Pérez; Tamara Heartsill Scalley

2008-01-01

In Puerto Rico, brackish water wetlands were dominated by Pterocarpus officinalis previous to extensive deforestation due to agriculture. Today remnant wetlands are limited to small areas that are threatened by rise in sea level. We examined the root nodules of P. officinalis in montane and coastal sites and at 0, 10, 20 cm from the surface to determine if site...

Results of 30-year-old plantations on surface mines in the Central States

Treesearch

W. Clark Ashby; Clay A. Kolar; Nelson F. Rogers

1980-01-01

Twenty-eight tree species have grown successfully on surface-mined lands in the Central States. Variability in species performance can be related to geographic area, type of rooting medium, and associated species. Many planted stands have been vigorously invaded by volunteer trees, as well as by other plants and animals.

Healing of experimental apical periodontitis after apicoectomy using different sealing materials on the resected root end.

PubMed

Otani, Kaori; Sugaya, Tsutomu; Tomita, Mahito; Hasegawa, Yukiko; Miyaji, Hirofumi; Tenkumo, Taichi; Tanaka, Saori; Motoki, Youji; Takanawa, Yasuhiro; Kawanami, Masamitsu

2011-01-01

This study evaluated apical periodontal healing after root-end sealing using 4-META/MMA-TBB resin (SB), and root-end filling using reinforced zinc oxide eugenol cement (EBA) or mineral trioxide aggregate (MTA) when root canal infection persisted. Apical periodontitis was induced in mandibular premolars of beagles by contaminating the root canals with dental plaque. After 1 month, in the SB group, SB was applied to the resected surface following apicoectomy. In the EBA and MTA groups, a root-end cavity was prepared and filled with EBA or MTA. In the control group, the root-end was not filled. Fourteen weeks after surgery, histological and radiographic analyses in a beagle model were performed. The bone defect area in the SB, EBA and MTA groups was significantly smaller than that in the control group. The result indicated that root-end sealing using SB and root-end filling using EBA or MTA are significantly better than control.

Lithium chloride attenuates root resorption during orthodontic tooth movement in rats.

PubMed

Wang, Yu; Gao, Shang; Jiang, Huan; Lin, Peng; Bao, Xingfu; Zhang, Zhimin; Hu, Min

2014-02-01

Root resorption is a common side effect of orthodontic treatment. In the current study, lithium chloride (LiCl), a Wnt signaling activator, was examined to determine its effect on root resorption. In total, 10 Sprague Dawley rats were randomly allocated into the experimental group (EG) and control group (CG). Each group consisted of five subjects. By using closed nickel-titanium coil springs, a 50-g force was applied between the upper incisors and the maxillary right first molars in order to mimic orthodontic biomechanics in the EG and CG for 14 days. During the 14 days, the EG rats were gavage-fed 200 mg/kg LiCl every 48 h. Next, digital radiographs were captured using a micro-computational tomography scanner. The movement of the maxillary first molars and the root resorption area ratio were measured electronically on the digital radiographs. The outcomes were analyzed using ANOVA. Following 14 days of experimental force application, all rats had spaces of varying sizes between the first and second right maxillary molars. The average distance measured in the CG was slightly higher than in the EG, however, the difference was not found to be statistically significant (P=0.224). Root resorption craters were observed in the groups following the experiment. Rough cementum areas were observed on the mesial surface of the distobuccal and distopalatal roots. The mean root resorption area ratio of CG was significantly greater than EG (P<0.05). Results of the present study indicate that LiCl can attenuate orthodontically induce root resorption during orthodontic tooth movement. The effect of LiCl on tooth movement is insignificant.

Analysis of Loss of Ignition of Root Zone of Bioretention Units at the Edison Environmental Center

EPA Science Inventory

Six bioretention units (widths 7.1 m) at USEPA’s Edison Environmental Center were constructed to evaluate sizing of surface area to watershed area. Three sizes were tested in duplicate with changes in aspect ratio of length from inlet (northern) wall by doubling successive ...

Reducing Strength Prevailing at Root Surface of Plants Promotes Reduction of Ag+ and Generation of Ag0/Ag2O Nanoparticles Exogenously in Aqueous Phase

PubMed Central

Pardha-Saradhi, Peddisetty; Yamal, Gupta; Peddisetty, Tanuj; Sharmila, Peddisetty; Nagar, Shilpi; Singh, Jyoti; Nagarajan, Rajamani; Rao, Kottapalli S.

2014-01-01

Potential of root system of plants from wide range of families to effectively reduce membrane impermeable ferricyanide to ferrocyanide and blue coloured 2,6-dichlorophenol indophenol (DCPIP) to colourless DCPIPH2 both under non-sterile and sterile conditions, revealed prevalence of immense reducing strength at root surface. As generation of silver nanoparticles (NPs) from Ag+ involves reduction, present investigations were carried to evaluate if reducing strength prevailing at surface of root system can be exploited for reduction of Ag+ and exogenous generation of silver-NPs. Root system of intact plants of 16 species from 11 diverse families of angiosperms turned clear colorless AgNO3 solutions, turbid brown. Absorption spectra of these turbid brown solutions showed silver-NPs specific surface plasmon resonance peak. Transmission electron microscope coupled with energy dispersive X-ray confirmed the presence of distinct NPs in the range of 5–50 nm containing Ag. Selected area electron diffraction and powder X-ray diffraction patterns of the silver NPs showed Bragg reflections, characteristic of crystalline face-centered cubic structure of Ag0 and cubic structure of Ag2O. Root system of intact plants raised under sterile conditions also generated Ag0/Ag2O-NPs under strict sterile conditions in a manner similar to that recorded under non-sterile conditions. This revealed the inbuilt potential of root system to generate Ag0/Ag2O-NPs independent of any microorganism. Roots of intact plants reduced triphenyltetrazolium to triphenylformazon and impermeable ferricyanide to ferrocyanide, suggesting involvement of plasma membrane bound dehydrogenases in reduction of Ag+ and formation of Ag0/Ag2O-NPs. Root enzyme extract reduced triphenyltetrazolium to triphenylformazon and Ag+ to Ag0 in presence of NADH, clearly establishing potential of dehydrogenases to reduce Ag+ to Ag0, which generate Ag0/Ag2O-NPs. Findings presented in this manuscript put forth a novel, simple, economically viable and green protocol for synthesis of silver-NPs under ambient conditions in aqueous phase, using root system of intact plants. PMID:25184239

Mercury uptake and phytotoxicity in terrestrial plants grown naturally in the Gumuskoy (Kutahya) mining area, Turkey.

PubMed

Sasmaz, Merve; Akgül, Bunyamin; Yıldırım, Derya; Sasmaz, Ahmet

2016-01-01

This study investigated mercury (Hg) uptake and transport from the soil to different plant parts by documenting the distribution and accumulation of Hg in the roots and shoots of 12 terrestrial plant species, all of which grow naturally in surface soils of the Gumuskoy Pb-Ag mining area. Plant samples and their associated soils were collected and analyzed for Hg content by ICP-MS. Mean Hg values in the soils, roots, and shoots of all plants were 6.914, 460, and 206 µg kg(-1), respectively and lower than 1. The mean enrichment factors for the roots (ECR) and shoots (ECS) of these plants were 0.06 and 0.09, respectively and lower than 1. These results show that the roots of the studied plants prevented Hg from reaching the aerial parts of the plants. The mean translocation factor (TLF) was 1.29 and higher than 1. The mean TLF values indicated that all 12 plant species had the ability to transfer Hg from the roots to the shoots but that transfer was more efficient in plants with higher ECR and ECS. Therefore, these plants could be useful for the biomonitoring of environmental pollution and for rehabilitating areas contaminated by Hg.

Changing spatial patterns of evapotranspiration and deep drainage in response to the interactions among impervious surface arrangement, soil characteristics, and weather on a residential parcel.

NASA Astrophysics Data System (ADS)

Voter, C. B.; Steven, L. I.

2015-12-01

The introduction impervious surfaces in urban areas is a key driver of hydrologic change. It is now well understood that the amount of "effective" impervious area directly connected to the storm sewer network is a better indicator of hydrologic behavior than the total amount of impervious area. Most studies in urban hydrology have focused on the relationship between impervious connectivity and stormwater runoff or other surface water flows, with the result that the effect on subsurface flow is not as well understood. In the field, we observe differences in soil moisture availability that are dependent on proximity to impervious features and significant from a root water uptake perspective, which indicates that parcel-scale subsurface and plant water fluxes may also be sensitive to fine-scaled heterogeneity in impervious surface arrangement and connectivity. We use ParFlow with CLM, a watershed model with fully integrated variably-saturated subsurface flow, overland flow, and land-surface processes, to explore the extent to which soil moisture, evapotranspiration, and deep drainage vary under various impervious surface arrangement and soil condition scenarios, as well as under a range of precipitation regimes. We investigate the effect of several impervious surface and soil characteristics, including general lot layout, downspout disconnect, and direction of driveway/sidewalk slope, and soil compaction. We show that that some impervious connectivity schemes transfer more water from impervious areas to pervious ones and promote localized recharge by developing well-defined, fast-moving wetting fronts that are able to penetrate the root zone. Enhanced infiltration is translated more directly to recharge in normal to wet years but partitioned more often to transpiration in dry years, leading to a nonlinear relationship among precipitation, runoff and recharge.

Effects of clodronate on early alveolar bone remodeling and root resorption related to orthodontic forces: a histomorphometric analysis.

PubMed

Choi, Josefina; Baek, Seung-Hak; Lee, Jae-Il; Chang, Young-Il

2010-11-01

The objective of this study was to evaluate the short-term effects of clodronate, a first-generation bisphosphonate, on early alveolar bone remodeling and root resorption related to orthodontic tooth movement. The samples consisted of 54 sex-matched Wistar rats (weight, 180-230 g) allocated to the 2.5 mmol/L clodronate, 10 mmol/L clodronate, and control groups (n = 18 for each group). After application of a nickel-titanium closed-coil spring (force, 60 g) between the maxillary central incisor and first molar, 2.5 mmol/L of clodronate, 10 mmol/L of clodronate, or saline solution was injected into the subperiosteum adjacent to the maxillary first molar every third day. All animals received tetracycline, calcein, and alizarin red by intraperitoneal injection at 1, 6, and 14 days, respectively. The amounts of tooth movement were measured at 3, 6, 9, 12, and 15 days. The animals were killed at 4, 7, and 17 days. Histomorphometric analyses of bone mineral appositional rate, labeled surface, percentage of root resorption area, and number of root resorption lacunae of the mesiobuccal root of the maxillary first molar at 4, 7, and 17 days were done. One-way analysis of variance (ANOVA) with the post-hoc test were done for statistical analyses. Rats in the 10 mmol/L clodronate group had significant decreases of tooth movement (12 and 15 days, P <0.05) and percentages of root resorption area and numbers of root resorption lacunae (7 day, P <0.05), and increases of labeled surface and mineral appositional rates (17 day, P <0.05) over those of the 2.5 mmol/L clodronate and control groups. Although clodronate might decrease root resorption related to orthodontic tooth movement, patients should be informed about a possible decrease in the amount of tooth movement and a prolonged period of orthodontic treatment. Copyright © 2010 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved.

Incorporating dynamic root growth enhances the performance of Noah-MP at two contrasting winter wheat field sites

NASA Astrophysics Data System (ADS)

Gayler, Sebastian; Wöhling, Thomas; Ingwersen, Joachim; Wizemann, Hans-Dieter; Warrach-Sagi, Kirsten; Attinger, Sabine; Streck, Thilo; Wulmeyer, Volker

2014-05-01

Interactions between the soil, the vegetation, and the atmospheric boundary layer require close attention when predicting water fluxes in the hydrogeosystem, agricultural systems, weather and climate. However, land-surface schemes used in large scale models continue to show deficits in consistently simulating fluxes of water and energy from the subsurface through vegetation layers to the atmosphere. In this study, the multi-physics version of the Noah land-surface model (Noah-MP) was used to identify the processes, which are most crucial for a simultaneous simulation of water and heat fluxes between land-surface and the lower atmosphere. Comprehensive field data sets of latent and sensible heat fluxes, ground heat flux, soil moisture, and leaf area index from two contrasting field sites in South-West Germany are used to assess the accuracy of simulations. It is shown that an adequate representation of vegetation-related processes is the most important control for a consistent simulation of energy and water fluxes in the soil-plant-atmosphere system. In particular, using a newly implemented sub-module to simulate root growth dynamics has enhanced the performance of Noah-MP at both field sites. We conclude that further advances in the representation of leaf area dynamics and root/soil moisture interactions are the most promising starting points for improving the simulation of feedbacks between the sub-soil, land-surface and atmosphere in fully-coupled hydrological and atmospheric models.

[Effects of neighbor competition on growth, fine root morphology and distribution of Schima superba and Cunninghamia lanceolata in different nutrient environments].

PubMed

Yao, Jia Bao; Chu, Xiu Li; Zhou, Zhi Chun; Tong, Jian She; Wang, Hui; Yu, Jia Zhong

2017-05-18

Taking Schima superba and Cunninghamia lanceolata as test materials, a pot experiment was conducted to simulate the heterogeneous and homogeneous forest soil nutrient environments, and design three planting modes including single plant, two-strain pure plant and two-strain mixed ones to reason the promotion in mixed S. superba and C. lanceolata plantation and the competitive advantage of S. superba. Results showed that compared with the homogeneous nutrient environment, both S. superba and C. lanceolata had the higher seedling height and dry matter accumulation, when mixed in the heterogeneous nutrient environment, S. superba displayed the obviously competitive advantage, which related to its root plasticity. The fine root of S. superba mixed in each diameter class showed a lot of hyperplasia, and the root total length, surface area and volume of which were 80%-180% higher than that of C. lanceolata. S. superba took the advantage of the compensatory growth strategy of vertical direction in fine roots, namely, they still multiplied to gain greater competitive advantage in low nutrient patches, besides occupying eutrophic surface. The different soil colonization and niche differentiation in fine root of S. superba and C. lanceolata alleviated the strong competition for nutrients of the roots of the two species, and improved the mixed-plantation production. Pure plantation of S. superba harvested the lower yield, which due to the root self-recognition inhibited the growth of root system. Fine roots staggered and evenly distributed on the space might be a reason for stable structure of pure S. superba plantation. So, it was recommended that block surface layer soil preparation and fertilization are used to improve the soil nutrient distribution, and the mixed plantation is constructed to promote the growth of S. superba and C. lanceolata, at the same time, the stand density is regulated to promote tree growth for the pure artificial S. superba plantation which had already been built.

[Effects of ridge-cultivation and plastic film mulching on root distribution and yield of spring maize in hilly area of central Sichuan basin, China.

PubMed

Zha, Li; Xie, Meng Lin; Zhu, Min; Dou, Pan; Cheng, Qiu Bo; Wang, Xing Long; Yuan, Ji Chao; Kong, Fan Lei

2016-03-01

A field experiment was conducted to study the effects of planting pattern (ridge culture, flatten culture, furrow culture) and film mulching on the distribution of spring maize root system and their influence on the yield of spring maize in the hilly area of central Sichuan basin. The results showed that ridge and film mulching had great influence on root morphology and root distribution of maize. The root length, root surface area and root volume of film mulching was 42.3%, 50.0%, 57.4% higher than those of no film mulching at jointing stage. The film mulching significantly increased the dry mass of root in vertical and horizontal distribution, and increased the root allocation ratio in deeper soil layer (20-40 cm) and the allocation ratio of wide row (0-20 cm) in horizontal direction. The effects of planting pattern on root growth and root distribution differed by film mulching. With film mulching, the ridge culture significantly increased the root dry mass in each soil layer and enlarged the distribution percentage of wide row (20-40 cm) in horizontal direction, as well as the dry mass of root in horizontal distribution and the root allocation ratio of wide row. The root mass under film mulching was in the order of ridge culture>flatten culture>furrow culture. Without film mulching, the furrow culture significantly increased root dry mass of narrow row (0-40 cm), and the root mass under no film mulching was in the order of furrow culture > ridge culture >flatten culture. As for the spike characteristics and maize yield, the filming mulching mea-sures reduced the corn bald length while increased the spike length, grain number, 1000-grain mass and yield. The yield under film mulching was in the order of ridge culture>flatten culture> furrow culture, while it was furrow culture > flatten culture > ridge culture under no film mulching. The reason for yield increase under ridge culture with film mulching was that it increased root weight especially in deep soil, and promoted the root allocation ratio in deeper soil and wide row (20-40 cm) in horizontal direction. The ridge-furrow culture without film mulching was helpful to root growth and increased the maize yield.

Degenerative alterations of the cementum-periodontal ligament complex and early tooth loss in a young patient with periodontal disease.

PubMed

Petruţiu, S A; Buiga, Petronela; Roman, Alexandra; Danciu, Theodora; Mihu, Carmen Mihaela; Mihu, D

2012-01-01

Premature exfoliation of primary or permanent teeth in children or adolescents is extremely rare and it can be a manifestation of an underlying systemic disease. This study aims to present the histological aspects associated with early tooth loss in a case of periodontal disease developed without local inflammation and with minimal periodontal pockets and attachment loss. The maxillary left second premolar was extracted together with a gingival collar attached to the root surface. The histological analysis recorded the resorption of the cementum in multiple areas of the entire root surface with the connective tissue of the desmodontium invading the lacunae defects. The connective tissue rich in cells occupied the periodontal ligamentar space and the resorptive areas. No inflammation was obvious in the periodontal ligament connective tissue. This report may warn clinicians about the possibility of the association of cemental abnormalities with early tooth loss.

Effects of arbuscular mycorrhizal fungi on seedling growth and development of two wetland plants, Bidens frondosa L., and Eclipta prostrata (L.) L., grown under three levels of water availability.

PubMed

Stevens, Kevin J; Wall, Christopher B; Janssen, Joel A

2011-05-01

To identify the importance of arbuscular mycorrhizal fungi (AMF) colonizing wetland seedlings following flooding, we assessed the effects of AMF on seedling establishment of two pioneer species, Bidens frondosa and Eclipta prostrata grown under three levels of water availability and ask: (1) Do inoculated seedlings differ in growth and development from non-inoculated plants? (2) Are the effects of inoculation and degree of colonization dependent on water availability? (3) Do plant responses to inoculation differ between two closely related species? Inoculation had no detectable effects on shoot height, or plant biomass but did affect biomass partitioning and root morphology in a species-specific manner. Shoot/root ratios were significantly lower in non-inoculated E. prostrata plants compared with inoculated plants (0.381 ± 0.066 vs. 0.683 ± 0.132). Root length and surface area were greater in non-inoculated E. prostrata (259.55 ± 33.78 cm vs. 194.64 ± 27.45 cm and 54.91 ± 7.628 cm(2) vs. 46.26 ± 6.8 cm(2), respectively). Inoculation had no detectable effect on B. frondosa root length, volume, or surface area. AMF associations formed at all levels of water availability. Hyphal, arbuscular, and vesicular colonization levels were greater in dry compared with intermediate and flooded treatments. Measures of mycorrhizal responsiveness were significantly depressed in E. prostrata compared with B. frondosa for total fresh weight (-0.3 ± 0.18 g vs. 0.06 ± 0.06 g), root length (-0.78 ± 0.28 cm vs.-0.11 ± 0.07 cm), root volume (-0.49 ± 0.22 cm(3) vs. 0.06 ± 0.07 cm(3)), and surface area (-0.59 ± 0.23 cm(2) vs.-0.03 ± 0.08 cm(2)). Given the disparity in species response to AMF inoculation, events that alter AMF prevalence in wetlands could significantly alter plant community structure by directly affecting seedling growth and development.

Physical properties of root cementum: Part I. A new method for 3-dimensional evaluation.

PubMed

Malek, S; Darendeliler, M A; Swain, M V

2001-08-01

Cementum is a nonuniform connective tissue that covers the roots of human teeth. Investigation of the physical properties of cementum may help in understanding or evaluating any possible connection to root resorption. A variety of engineering tests are available to investigate these properties. However, the thickness of the cementum layer varies, and this limits the applicability of these techniques in determining the physical properties of cementum. Hardness testing with Knoop and Vickers indentations overcame some of these limitations, but they prohibited the retrieval and retesting of the sample and therefore the testing was restricted to one area or section of the tooth. Another limiting factor with the existing techniques was the risk of artifacts related to the embedding material such as acrylic. A new method to investigate the physical properties of human premolar cementum was developed to obtain a 3-dimensional map of these properties with the Ultra Micro Indentation System (UMIS-2000; Commonwealth Scientific and Industrial Research Organization, Campbell, Australia). UMIS-2000 is a nano-indentation instrument for investigation of the properties of the near-surface region of materials. Premolars were harvested from orthodontic patients requiring extractions and then mounted on a newly designed surveyor that allowed sample retrieval and 3-dimensional rotation. This novel method enabled the quantitative testing of root surface cementum, on all 4 root surfaces, extending from the apex to the cementoenamel junction at 60 different sites.

Comparison of progressive addition lenses by direct measurement of surface shape.

PubMed

Huang, Ching-Yao; Raasch, Thomas W; Yi, Allen Y; Bullimore, Mark A

2013-06-01

To compare the optical properties of five state-of-the-art progressive addition lenses (PALs) by direct physical measurement of surface shape. Five contemporary freeform PALs (Varilux Comfort Enhanced, Varilux Physio Enhanced, Hoya Lifestyle, Shamir Autograph, and Zeiss Individual) with plano distance power and a +2.00-diopter add were measured with a coordinate measuring machine. The front and back surface heights were physically measured, and the optical properties of each surface, and their combination, were calculated with custom MATLAB routines. Surface shape was described as the sum of Zernike polynomials. Progressive addition lenses were represented as contour plots of spherical equivalent power, cylindrical power, and higher order aberrations (HOAs). Maximum power rate, minimum 1.00-DC corridor width, percentage of lens area with less than 1.00 DC, and root mean square of HOAs were also compared. Comfort Enhanced and Physio Enhanced have freeform front surfaces, Shamir Autograph and Zeiss Individual have freeform back surfaces, and Hoya Lifestyle has freeform properties on both surfaces. However, the overall optical properties are similar, regardless of the lens design. The maximum power rate is between 0.08 and 0.12 diopters per millimeter and the minimum corridor width is between 8 and 11 mm. For a 40-mm lens diameter, the percentage of lens area with less than 1.00 DC is between 64 and 76%. The third-order Zernike terms are the dominant high-order terms in HOAs (78 to 93% of overall shape variance). Higher order aberrations are higher along the corridor area and around the near zone. The maximum root mean square of HOAs based on a 4.5-mm pupil size around the corridor area is between 0.05 and 0.06 µm. This nonoptical method using a coordinate measuring machine can be used to evaluate a PAL by surface height measurements, with the optical properties directly related to its front and back surface designs.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Suresh, Niraj; Stephens, Sean A.; Adams, Lexor

Plant roots play a critical role in plant-soil-microbe interactions that occur in the rhizosphere, as well as processes with important implications to climate change and forest management. Quantitative size information on roots in their native environment is invaluable for studying root growth and environmental processes involving the plant. X ray computed tomography (XCT) has been demonstrated to be an effective tool for in situ root scanning and analysis. Our group at the Environmental Molecular Sciences Laboratory (EMSL) has developed an XCT-based tool to image and quantitatively analyze plant root structures in their native soil environment. XCT data collected on amore » Prairie dropseed (Sporobolus heterolepis) specimen was used to visualize its root structure. A combination of open-source software RooTrak and DDV were employed to segment the root from the soil, and calculate its isosurface, respectively. Our own computer script named 3DRoot-SV was developed and used to calculate root volume and surface area from a triangular mesh. The process utilizing a unique combination of tools, from imaging to quantitative root analysis, including the 3DRoot-SV computer script, is described.« less

Rapid Phenotyping of Root Systems of Brachypodium Plants Using X-ray Computed Tomography: a Comparative Study of Soil Types and Segmentation Tools

NASA Astrophysics Data System (ADS)

Varga, T.; McKinney, A. L.; Bingham, E.; Handakumbura, P. P.; Jansson, C.

2017-12-01

Plant roots play a critical role in plant-soil-microbe interactions that occur in the rhizosphere, as well as in processes with important implications to farming and thus human food supply. X-ray computed tomography (XCT) has been proven to be an effective tool for non-invasive root imaging and analysis. Selected Brachypodium distachyon phenotypes were grown in both natural and artificial soil mixes. The specimens were imaged by XCT, and the root architectures were extracted from the data using three different software-based methods; RooTrak, ImageJ-based WEKA segmentation, and the segmentation feature in VG Studio MAX. The 3D root image was successfully segmented at 30 µm resolution by all three methods. In this presentation, ease of segmentation and the accuracy of the extracted quantitative information (root volume and surface area) will be compared between soil types and segmentation methods. The best route to easy and accurate segmentation and root analysis will be highlighted.

Dynamic root distributions in ecohydrological modeling: A case study at Walnut Gulch Experimental Watershed

NASA Astrophysics Data System (ADS)

Sivandran, Gajan; Bras, Rafael L.

2013-06-01

Arid regions are characterized by high variability in the arrival of rainfall, and species found in these areas have adapted mechanisms to ensure the capture of this scarce resource. In particular, the rooting strategies employed by vegetation can be critical to their survival. However, land surface models currently prescribe rooting profiles as a function of only the plant functional type of interest with no consideration for the soil texture or rainfall regime of the region being modeled. Additionally, these models do not incorporate the ability of vegetation to dynamically alter their rooting strategies in response to transient changes in environmental forcings or competition from other plant species and therefore tend to underestimate the resilience of these ecosystems. To address the simplicity of the current representation of roots in land surface models, a new dynamic rooting scheme was incorporated into the framework of the distributed ecohydrological model tRIBS+VEGGIE. The new scheme optimizes the allocation of carbon to the root zone to reduce the perceived stress of the vegetation, so that root profiles evolve based upon local climate and soil conditions. The ability of the new scheme to capture the complex dynamics of natural systems was evaluated by comparisons to hourly timescale energy flux, soil moisture, and vegetation growth observations from the Walnut Gulch Experimental Watershed, Arizona. Robust agreement was found between the model and observations, providing confidence that the improved model is able to capture the multidirectional interactions between climate, soil, and vegetation at this site.

Isolation and characterization of N2 -fixing bacteria from giant reed and switchgrass for plant growth promotion and nutrient uptake.

PubMed

Xu, Jia; Kloepper, Joseph W; Huang, Ping; McInroy, John A; Hu, Chia H

2018-05-01

The aims of this study were to isolate and characterize N 2 -fixing bacteria from giant reed and switchgrass and evaluate their plant growth promotion and nutrient uptake potential for use as biofertilizers. A total of 190 bacteria were obtained from rhizosphere soil and inside stems and roots of giant reed and switchgrass. All the isolates were confirmed to have nitrogenase activity, 96.9% produced auxin, and 85% produced siderophores. Then the top six strains, including Sphingomonas trueperi NNA-14, Sphingomonas trueperi NNA-19, Sphingomonas trueperi NNA-17, Sphingomonas trueperi NNA-20, Psychrobacillus psychrodurans NP-3, and Enterobacter oryzae NXU-38, based on nitrogenase activity, were inoculated on maize and wheat seeds in greenhouse tests to assess their potential benefits to plants. All the selected strains promoted plant growth by increasing at least one plant growth parameter or increasing the nutrient concentration of maize or wheat plants. NNA-14 outperformed others in promoting early growth and nutrient uptake by maize. Specifically, NNA-14 significantly increased root length, surface area, and fine roots of maize by 14%, 12%, and 17%, respectively, and enhanced N, Ca, S, B, Cu, and Zn in maize. NNA-19 and NXU-38 outperformed others in promoting both early growth and nutrient uptake by wheat. Specifically, NNA-19 significantly increased root dry weight and number of root tips of wheat by 25% and 96%, respectively, and enhanced Ca in wheat. NXU-38 significantly increased root length, surface area, and fine roots of wheat by 21%, 13%, and 26%, respectively, and enhanced levels of Ca and Mg in wheat. It is concluded that switchgrass and giant reed are colonized by N 2 -fixing bacteria that have the potential to contribute to plant growth and nutrient uptake by agricultural crops. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KSC-04pd1286

NASA Image and Video Library

2004-06-10

KENNEDY SPACE CENTER, FLA. - A young wild pig roots in the grass for food in an area near the NASA News Center at KSC. Feral pigs were introduced to Florida in the 1500s and are now found statewide in wooded areas close to water. The pigs have flourished in the environs around KSC, which shares a border with the Merritt Island National Wildlife Refuge, without many predators other than panthers and humans. Pigs are omnivores, foraging on the ground and rooting just beneath the surface, which damages the groundcover. Wild pigs eat almost anything that has nutritional value, including tubers, roots, shoots, acorns, fruits, berries, earthworms, amphibians, reptiles and rodents. Appearance is similar to domestic hogs, but leaner, with a longer, narrower head and a coarser, denser coat. Females may have two litters per year. The piglets are weaned in a few weeks but remain with the mother for several months.

KSC-04PD-1286

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. A young wild pig roots in the grass for food in an area near the NASA News Center at KSC. Feral pigs were introduced to Florida in the 1500s and are now found statewide in wooded areas close to water. The pigs have flourished in the environs around KSC, which shares a border with the Merritt Island National Wildlife Refuge, without many predators other than panthers and humans. Pigs are omnivores, foraging on the ground and rooting just beneath the surface, which damages the groundcover. Wild pigs eat almost anything that has nutritional value, including tubers, roots, shoots, acorns, fruits, berries, earthworms, amphibians, reptiles and rodents. Appearance is similar to domestic hogs, but leaner, with a longer, narrower head and a coarser, denser coat. Females may have two litters per year. The piglets are weaned in a few weeks but remain with the mother for several months.

Miniature endoscopic optical coherence tomography for calculus detection.

PubMed

Kao, Meng-Chun; Lin, Chun-Li; Kung, Che-Yen; Huang, Yi-Fung; Kuo, Wen-Chuan

2015-08-20

The effective treatment of periodontitis involves the detection and removal of subgingival dental calculus. However, subgingival calculus is more difficult to detect than supragingival calculus because it is firmly attached to root surfaces within periodontal pockets. To achieve a smooth root surface, clinicians often remove excessive amounts of root structure because of decreased visibility. In addition, enamel pearl, a rare type of ectopic enamel formation on the root surface, can easily be confused with dental calculus in the subgingival environment. In this study, we developed a fiber-probe swept-source optical coherence tomography (SSOCT) technique and combined it with the quantitative measurement of an optical parameter [standard deviation (SD) of the optical coherence tomography (OCT) intensity] to differentiate subgingival calculus from sound enamel, including enamel pearl. Two-dimensional circumferential images were constructed by rotating the miniprobe (0.9 mm diameter) while acquiring image lines, and the adjacent lines in each rotation were stacked to generate a three-dimensional volume. In OCT images, compared to sound enamel and enamel pearls, dental calculus showed significant differences (P<0.001) in SD values. Finally, the receiver operating characteristic curve had a high capacity (area under the curve=0.934) for discriminating between healthy regions (including enamel pearl) and dental calculus.

Simulating land surface energy fluxes using a microscopic root water uptake approach in a northern temperate forest

NASA Astrophysics Data System (ADS)

He, L.; Ivanov, V. Y.; Schneider, C.

2012-12-01

The predictive accuracy of current land surface models has been limited by uncertainties in modeling transpiration and its sensitivity to the plant-available water in the root zone. Models usually distribute vegetation transpiration demand as sink terms in one-dimensional soil-water accounting model, according to the vertical root density profile. During water-limited situations, the sink terms are constrained using a heuristic "Feddes-type" water stress function. This approach significantly simplifies the actual three-dimensional physical process of root water uptake and may predict an early onset of water-limited transpiration. Recently, a microscopic root water uptake approach was proposed to simulate the three-dimensional radial moisture fluxes from the soil to roots, and water flux transfer processes along the root systems. During dry conditions, this approach permits the compensation of decreased root water uptake in water-stressed regions by increasing uptake density in moister regions. This effect cannot be captured by the Feddes heuristic function. This study "loosely" incorporates the microscopic root water uptake approach based on aRoot model into an ecohydrological model tRIBS+VEGGIE. The ecohydrological model provides boundary conditions for the microscopic root water uptake model (e.g., potential transpiration, soil evaporation, and precipitation influx), and the latter computes the actual transpiration and profiles of sink terms. Based on the departure of the actual latent heat flux from the potential value, the other energy budget components are adjusted. The study is conducted for a northern temperate mixed forest near the University of Michigan Biological Station. Observational evidence for this site suggests little-to-no control of transpiration by soil moisture yet the commonly used Feddes-type approach implies severe water limitation on transpiration during dry episodes. The study addresses two species: oak and aspen. The effects of differences in root architecture on actual transpiration are explored. The energy components simulated with the microscopic modeling approach are tested against observational data. Through the improved spatiotemporal representation of small-scale root water uptake process, the microscopic modeling framework leads to a better agreement with the observational data than the Feddes-type approach. During dry periods, relatively high transpiration is sustained, as water uptake regions shift from densely to sparsely rooted layers, or from drier to moister soil areas. Implications and approaches for incorporating microscopic modeling methodologies within large-scale land-surface parameterizations are discussed.

Minimizing the area required for time constants in integrated circuits

NASA Technical Reports Server (NTRS)

Lyons, J. C.

1972-01-01

When a medium- or large-scale integrated circuit is designed, efforts are usually made to avoid the use of resistor-capacitor time constant generators. The capacitor needed for this circuit usually takes up more surface area on the chip than several resistors and transistors. When the use of this network is unavoidable, the designer usually makes an effort to see that the choice of resistor and capacitor combinations is such that a minimum amount of surface area is consumed. The optimum ratio of resistance to capacitance that will result in this minimum area is equal to the ratio of resistance to capacitance which may be obtained from a unit of surface area for the particular process being used. The minimum area required is a function of the square root of the reciprocal of the products of the resistance and capacitance per unit area. This minimum occurs when the area required by the resistor is equal to the area required by the capacitor.

Responses of root physiological characteristics and yield of sweet potato to humic acid urea fertilizer

PubMed Central

Kou, Meng; Tang, Zhonghou; Zhang, Aijun; Li, Hongmin; Wei, Meng

2017-01-01

Humic acid (HA), not only promote the growth of crop roots, they can be combined with nitrogen (N) to increase fertilizer use efficiency and yield. However, the effects of HA urea fertilizer (HA-N) on root growth and yield of sweet potato has not been widely investigated. Xushu 28 was used as the experimental crop to investigate the effects of HA-N on root morphology, active oxygen metabolism and yield under field conditions. Results showed that nitrogen application alone was not beneficial for root growth and storage root formation during the early growth stage. HA-N significantly increased the dry weight of the root system, promoted differentiation from adventitious root to storage root, and increased the overall root activity, total root length, root diameter, root surface area, as well as root volume. HA-N thus increased the activity of superoxide dismutase (SOD), peroxidase (POD), and Catalase (CAT) as well as increasing the soluble protein content of roots and decreasing the malondialdehyde (MDA) content. HA-N significantly increased both the number of storage roots per plant increased by 14.01%, and the average fresh weight per storage root increased by 13.7%, while the yield was also obviously increased by 29.56%. In this study, HA-N increased yield through a synergistic increase of biological yield and harvest index. PMID:29253886

Correlating Humidity-Dependent Ionically Conductive Surface Area with Transport Phenomena in Proton-Exchange Membranes

DOE Office of Scientific and Technical Information (OSTI.GOV)

He, Qinggang; Kusoglu, Ahmet; Lucas, Ivan T.

2011-08-01

The objective of this effort was to correlate the local surface ionic conductance of a Nafion? 212 proton-exchange membrane with its bulk and interfacial transport properties as a function of water content. Both macroscopic and microscopic proton conductivities were investigated at different relative humidity levels, using electrochemical impedance spectroscopy and current-sensing atomic force microscopy (CSAFM). We were able to identify small ion-conducting domains that grew with humidity at the surface of the membrane. Numerical analysis of the surface ionic conductance images recorded at various relative humidity levels helped determine the fractional area of ion-conducting active sites. A simple square-root relationshipmore » between the fractional conducting area and observed interfacial mass-transport resistance was established. Furthermore, the relationship between the bulk ionic conductivity and surface ionic conductance pattern of the Nafion? membrane was examined.« less

Identification of qSOR1, a major rice QTL involved in soil-surface rooting in paddy fields.

PubMed

Uga, Yusaku; Hanzawa, Eiko; Nagai, Shinsei; Sasaki, Kazuhiro; Yano, Masahiro; Sato, Tadashi

2012-01-01

Specific Indonesian lowland rice (Oryza sativa L.) cultivars elongate thick primary roots on the soil surface of paddy fields. To clarify the genetic factors controlling soil-surface rooting, we performed quantitative trait locus (QTL) analyses using 124 recombinant inbred lines (RILs) derived from a cross between Gemdjah Beton, an Indonesian lowland rice cultivar with soil-surface roots, and Sasanishiki, a Japanese lowland rice cultivar without soil-surface roots. These cultivars and the RILs were tested for soil-surface rooting in a paddy field. We identified four regions of chromosomes 3, 4, 6, and 7 that were associated with soil-surface rooting in the field. Among them, one major QTL was located on the long arm of chromosome 7. This QTL explained 32.5-53.6% of the total phenotypic variance across three field evaluations. To perform fine mapping of this QTL, we measured the basal root growth angle of crown roots at the seedling stage in seven BC(2)F(3) recombinant lines grown in small cups in a greenhouse. The QTL was mapped between markers RM21941 and RM21976, which delimit an 812-kb interval in the reference cultivar Nipponbare. We have designated this QTL qSOR1 (quantitative trait locus for SOIL SURFACE ROOTING 1).

Synergistic Effects of a Calcium Phosphate/Fibronectin Coating on the Adhesion of Periodontal Ligament Stem Cells Onto Decellularized Dental Root Surfaces.

PubMed

Lee, Jung-Seok; Kim, Hyun-Suk; Park, So-Yon; Kim, Tae-Wan; Jung, Jae-Suk; Lee, Jong-Bin; Kim, Chang-Sung

2015-01-01

This study aimed to enhance the attachment of periodontal ligament stem cells (PDLSCs) onto the decellularized dental root surface using surface coating with fibronectin and/or calcium phosphate (CaP) and to evaluate the activity of PDLSCs attached to a coated dental root surface following tooth replantation. PDLSCs were isolated from five dogs, and the other dental roots were used as a scaffold for carrying PDLSCs and then assigned to one of four groups according to whether their surface was coated with CaP, fibronectin, CaP/fibronectin, or left uncoated (control). Fibronectin increased the adhesion of PDLSCs onto dental root surfaces compared to both the control and CaP-coated groups, and simultaneous surface coating with CaP and fibronectin significantly accelerated and increased PDLSC adhesion compared to the fibronectin-only group. On in vivo tooth replantation, functionally oriented periodontal new attachment was observed on the CaP/fibronectin-coated dental roots to which autologous PDLSCs had adhered, while in the control condition, dental root replantation was associated only with root resorption and ankylosis along the entire root length. CaP and fibronectin synergistically enhanced the attachment of PDLSCs onto dental root surfaces, and autologous PDLSCs could produce de novo periodontal new attachment in an experimental in vivo model.

Bioremediation of phenolic compounds from water with plant root surface peroxidases

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adler, P.R.; Arora, R.; El Ghaouth, A.

1994-09-01

Peroxidases have been shown to polymerize phenolic compounds, thereby removing them from solution by precipitation. Others have studied the role of root surface associated peroxidases as a defense against fungal root pathogens; however, their use in detoxification of organic pollutants in vivo at the root surface has not been studied. Two plant species, waterhyacinth [Eichhornia crassipes (C. Mart) Solms-Laub.] and tomato (Lycopersicon esculentum L.), were tested for both in vitro and in vivo peroxidase activity on the root surface. In vitro studies indicated that root surface peroxidase activities were 181 and 78 nmol tetraguaiacol formed min{sup -1} g{sup -1} rootmore » fresh wt., for tomato and waterhyacinth, respectively. Light microscope studies revealed that guaiacol was polymerized in vivo at the root surface. Although peroxidase was evenly distributed on tomato roots, it was distributed patchily on waterhyacinth roots. In vitro studies using gas chromatography-mass spectrometry (GC-MS) showed that the efficiency of peroxidase to polymerize phenols vary with phenolic compound. We suggest that plants may be utilized as a source of peroxidases for removal of phenolic compounds that are on the EPA priority pollutant list and that root surface peroxidases may minimize the absorption of phenolic compounds into plants by precipitating them at the root surface. In this study we have identified a new use for root-associated proteins in ecologically engineering plant systems for bioremediation of phenolic compounds in the soil and water environment. 25 refs., 2 figs., 2 tabs.« less

Volatile signalling by sesquiterpenes from ectomycorrhizal fungi reprogrammes root architecture

PubMed Central

Ditengou, Franck A.; Müller, Anna; Rosenkranz, Maaria; Felten, Judith; Lasok, Hanna; van Doorn, Maja Miloradovic; Legué, Valerie; Palme, Klaus; Schnitzler, Jörg-Peter; Polle, Andrea

2015-01-01

The mutualistic association of roots with ectomycorrhizal fungi promotes plant health and is a hallmark of boreal and temperate forests worldwide. In the pre-colonization phase, before direct contact, lateral root (LR) production is massively stimulated, yet little is known about the signals exchanged during this step. Here, we identify sesquiterpenes (SQTs) as biologically active agents emitted by Laccaria bicolor while interacting with Populus or Arabidopsis. We show that inhibition of fungal SQT production by lovastatin strongly reduces LR proliferation and that (–)-thujopsene, a low-abundance SQT, is sufficient to stimulate LR formation in the absence of the fungus. Further, we show that the ectomycorrhizal ascomycote, Cenococcum geophilum, which cannot synthesize SQTs, does not promote LRs. We propose that the LR-promoting SQT signal creates a win-win situation by enhancing the root surface area for plant nutrient uptake and by improving fungal access to plant-derived carbon via root exudates. PMID:25703994

In vitro performance of DIAGNOdent laser fluorescence device for dental calculus detection on human tooth root surfaces.

PubMed

Rams, Thomas E; Alwaqyan, Abdulaziz Y

2017-10-01

This study assessed the reproducibility of a red diode laser device, and its capability to detect dental calculus in vitro on human tooth root surfaces. On each of 50 extracted teeth, a calculus-positive and calculus-free root surface was evaluated by two independent examiners with a low-power indium gallium arsenide phosphide diode laser (DIAGNOdent) fitted with a periodontal probe-like sapphire tip and emitting visible red light at 655 nm wavelength. Laser autofluorescence intensity readings of examined root surfaces were scored on a 0-99 scale, with duplicate assessments performed using the laser probe tip directed both perpendicular and parallel to evaluated tooth root surfaces. Pearson correlation coefficients of untransformed measurements, and kappa analysis of data dichotomized with a >40 autofluorescence intensity threshold, were calculated to assess intra- and inter-examiner reproducibility of the laser device. Mean autofluorescence intensity scores of calculus-positive and calculus-free root surfaces were evaluated with the Student's t -test. Excellent intra- and inter-examiner reproducibility was found for DIAGNOdent laser autofluorescence intensity measurements, with Pearson correlation coefficients above 94%, and kappa values ranging between 0.96 and 1.0, for duplicate readings taken with both laser probe tip orientations. Significantly higher autofluorescence intensity values were measured when the laser probe tip was directed perpendicular, rather than parallel, to tooth root surfaces. However, calculus-positive roots, particularly with calculus in markedly-raised ledges, yielded significantly greater mean DIAGNOdent laser autofluorescence intensity scores than calculus-free surfaces, regardless of probe tip orientation. DIAGNOdent autofluorescence intensity values >40 exhibited a stronger association with calculus (36.6 odds ratio) then measurements of ≥5 (20.1 odds ratio) when the laser probe tip was advanced parallel to root surfaces. Excellent intra- and inter-examiner reproducibility of autofluorescence intensity measurements was obtained with the DIAGNOdent laser fluorescence device on human tooth roots. Calculus-positive root surfaces exhibited significantly greater DIAGNOdent laser autofluorescence than calculus-free tooth roots, even with the laser probe tip directed parallel to root surfaces. These findings provide further in vitro validation of the potential utility of a DIAGNOdent laser fluorescence device for identifying dental calculus on human tooth root surfaces.

Prevalence of feral swine disturbance at important archaeological sites over a large landscape in Florida

NASA Astrophysics Data System (ADS)

Engeman, Richard M.; Meyer, Joseph S.; Allen, John B.

2017-01-01

Feral swine are globally known as one of the most destructive invasive vertebrates, damaging native habitats, native plants and animals, agriculture, infrastructure, spreading diseases. There has been little quantification on their disturbance to archaeological sites across a broad landscape. Over 6 years we inspected 293 significant archaeological sites for swine disturbance across a vast area. We found a 42% prevalence of swine disturbance among all sites, with prevalence not distinguishable among prehistoric sites, historic sites, and sites with both components. The areas of disturbance mapped within three historic homestead sites showed 5-26% of total site surface area rooted. Disturbance was not evident upon re-inspection of one of these sites after 18 months, indicating how evidence of disturbance can be obscured in this environment. Thus, our observed 42% prevalence of disturbance should be considered a minimum for disturbance occurring through time. Artifacts depths were <10 cm of the surface at 85% of the sites and <20 cm of the surface for 90% of the sites. Feral swine rooting commonly exceeds 20 cm in depth, especially in soft sandy substrates typical of Florida, making the great majority of the studied sites highly vulnerable to artifact damage or displacement.

Root Surface Bio-modification with Erbium Lasers- A Myth or a Reality??

PubMed Central

Lavu, Vamsi; Sundaram, Subramoniam; Sabarish, Ram; Rao, Suresh Ranga

2015-01-01

The objective of this literature review was to critically review the evidence available in the literature regarding the expediency of erbium family of lasers for root bio modification as a part of periodontal therapy. The literature search was performed on the Pubmed using MeSH words such as "lasers/therapeutic use, scaling, dental calculus, tooth root/anatomy and histology, ultrasonic therapy". The studies were screened and were grouped as follows: those evaluating a) efficacy for calculus removal with the Erbium family of laser b) root surface changes following Er YAG and Er Cr YSGG application c) comparative studies of the Er YAG, Er Cr YSGG lasers versus conventional methods of root surface modification d) Bio compatibility of root surface following Erbium laser treatment e) Studies on the combined efficacy of laser root modification with conventional methods towards root surface bio-modification f) Studies on effectiveness of root surface bio-modification prior to root coverage procedures. In conclusion, the erbium family has a proven anti-bacterial action, predictable calculus removal, minimal root substance removal, and appears to favor cell attachment. The Erbium family of lasers appears to be a useful adjunct for the management of periodontal disease. PMID:25713635

Tracking microbial colonization patterns associated with micro-environments of rice

NASA Astrophysics Data System (ADS)

Schmidt, Hannes; Eickhorst, Thilo

2015-04-01

The interface between soil and roots (i.e. the rhizosphere) represents a highly dynamic micro-environment for microbial populations. Root-derived compounds are released into the rhizosphere and may attract, stimulate, or inhibit native soil microorganisms. Microbes associated with the rhizosphere, in turn, may have deleterious, neutral, or promoting effects on the plant. Such influences of microbial populations on the plant and vice versa are likely to be greatest in close vicinity to the root surface. It is therefore essential to detect and visualize preferential micro-sites of microbial root colonization to identify potential areas of microbe-plant interaction. We present a single-cell based approach allowing for the localization, quantification, and visualization of native microbial populations in the rhizosphere and on the rhizoplane of soil-grown roots in situ. Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) in combination with confocal laser scanning microscopy was applied to observe colonization densities and patterns of microbial populations associated with wetland rice. Hybridizations with domain- and phylum-specific oligonucleotide probes showed that the growth stage of the rice plant as well as the distance to the root surface had a strong influence on microbial colonization patterns. Three-dimensional visualizations of root-associated microbes revealed micro-sites of preferential colonization. Highest cell numbers of archaea and bacteria were found at flowering stage of rice plant development. Irregular distribution patterns of microbiota observed at early growth stages shifted towards more uniform colonization with plant age. Accordingly, the highest colonization densities shifted from the tip to more mature regions of rice roots. Methanogenic archaea and methanotrophic bacteria were found to be co-localized at basal regions of lateral roots. Beneficial effects of a close association with root surfaces were indicated by proportionally higher numbers of methane-oxidizing bacteria on the rhizoplane compared to the rhizosphere. Such spatial effects could not be observed for methanogenic archaea. As a consequence, the detection and visualization of microbial colonization patterns on a micro-scale via CARD-FISH represents an instrumental approach in revealing potential sites of interaction between microbes and plants in soil micro-environments.

A micro-computed tomographic study of band-shaped root canal isthmuses, having their floor in the apical third of mesial roots of mandibular first molars.

PubMed

Keleş, A; Keskin, C

2018-02-01

To conduct a quantitative and qualitative analysis of the band-shaped isthmus area, the floor of which was in the apical third in the mesial roots of mandibular first molars using micro-computed tomography (micro-CT). Micro-CT images of 269 mesial roots of mandibular first molars were evaluated, and 40 specimens with a band-shaped isthmus, with a floor in the apical third, were selected. The major diameter, minor diameter, roundness, area and perimeter values for the most coronal and apical slices where the isthmus was visible were measured. The distances between these slices were measured as the isthmus length, and the total volume, structure model index and surface area of the isthmus were measured. The distances between the isthmus floor and two apical foramina and the number of root canal orifices were calculated. The dimensions of the isthmus roof and the floor were compared, and the data were analysed using descriptive statistics and Student's t-tests with a significance threshold set at 5%. A total of 15% of the specimens had band-shaped isthmuses with a floor in the apical third. The isthmus roof exhibited significantly greater major and minor diameter values compared to the isthmus floor (P < 0.05). No significant difference was detected between the isthmus roof and the floor with regard to roundness (P > 0.05). Three- and two-dimensional analyses of the mesial roots of mandibular molars revealed that band-shaped isthmuses had complex shapes. © 2017 International Endodontic Journal. Published by John Wiley & Sons Ltd.

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aufrecht, Jayde A.; Ryan, Jennifer M.; Hasim, Sahar

Root hairs increase root surface area for better water uptake and nutrient absorption by the plant. Because they are small in size and often obscured by their natural environment, root hair morphology and function are difficult to study and often excluded from plant research. In recent years, microfluidic platforms have offered a way to visualize root systems at high resolution without disturbing the roots during transfer to an imaging system. The microfluidic platform presented here builds on previous plant-on-a-chip research by incorporating a two-layer device to confine the Arabidopsis thaliana main root to the same optical plane as the rootmore » hairs. This design enables the quantification of root hairs on a cellular and organelle level and also prevents z-axis drifting during the addition of experimental treatments. We describe how to store the devices in a contained and hydrated environment, without the need for fluidic pumps, while maintaining a gnotobiotic environment for the seedling. After the optical imaging experiment, the device may be disassembled and used as a substrate for atomic force or scanning electron microscopy while keeping fine root structures intact.« less

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

DOE PAGES

Aufrecht, Jayde A.; Ryan, Jennifer M.; Hasim, Sahar; ...

2017-08-01

Root hairs increase root surface area for better water uptake and nutrient absorption by the plant. Because they are small in size and often obscured by their natural environment, root hair morphology and function are difficult to study and often excluded from plant research. In recent years, microfluidic platforms have offered a way to visualize root systems at high resolution without disturbing the roots during transfer to an imaging system. The microfluidic platform presented here builds on previous plant-on-a-chip research by incorporating a two-layer device to confine the Arabidopsis thaliana main root to the same optical plane as the rootmore » hairs. This design enables the quantification of root hairs on a cellular and organelle level and also prevents z-axis drifting during the addition of experimental treatments. We describe how to store the devices in a contained and hydrated environment, without the need for fluidic pumps, while maintaining a gnotobiotic environment for the seedling. After the optical imaging experiment, the device may be disassembled and used as a substrate for atomic force or scanning electron microscopy while keeping fine root structures intact.« less

Water uptake by seminal and adventitious roots in relation to whole-plant water flow in barley (Hordeum vulgare L.).

PubMed

Knipfer, Thorsten; Fricke, Wieland

2011-01-01

Prior to an assessment of the role of aquaporins in root water uptake, the main path of water movement in different types of root and driving forces during day and night need to be known. In the present study on hydroponically grown barley (Hordeum vulgare L.) the two main root types of 14- to 17-d-old plants were analysed for hydraulic conductivity in dependence of the main driving force (hydrostatic, osmotic). Seminal roots contributed 92% and adventitious roots 8% to plant water uptake. The lower contribution of adventitious compared with seminal roots was associated with a smaller surface area and number of roots per plant and a lower axial hydraulic conductance, and occurred despite a less-developed endodermis. The radial hydraulic conductivity of the two types of root was similar and depended little on the prevailing driving force, suggesting that water uptake occurred along a pathway that involved crossing of membrane(s). Exudation experiments showed that osmotic forces were sufficient to support night-time transpiration, yet transpiration experiments and cuticle permeance data questioned the significance of osmotic forces. During the day, 90% of water uptake was driven by a tension of about -0.15 MPa.

Study on the potential inhibition of root dentine wear adjacent to fluoride-containing restorations.

PubMed

Turssi, Cecilia Pedroso; Hara, Anderson Takeo; Domiciano, Silvia Jorge; Serra, Mônica Campos

2008-01-01

The purpose of this in vitro study was to determine whether the vicinity of root dentine that had been restored with fluoride-releasing materials was at reduced risk for erosive/abrasive wear compared to root dentine restored with a non-fluoride-containing material. According to a randomized complete block design, standardized cavities prepared on the surface of 150 bovine root dentine slabs were restored with glass-ionomer cement, resin-modified glass ionomer, polyacid-modified resin composite, fluoride-containing or conventional composite. Specimens were coated with two layers of an acid-resistant nail varnish exposing half of the dentine surface and half of the restoration. Subsequently, specimens were either eroded in an acidic drink or left uneroded, then exposed to artificial saliva and abraded in a toothbrushing machine. Wear depth in the vicinity of restorations was quantified by a stylus profilometer, based on the nonabraded areas surrounding the erosion/abrasion region. Two-way ANOVA did not demonstrate significant interaction between restoratives and eroded-uneroded dentine (p=0.5549) nor significant difference among restorative materials (p=0.8639). Tukey's test ascertained that the wear depth was higher for eroded than for uneroded groups. Fluoride-releasing materials seemed to negligibly inhibit wear in the vicinity of restored root dentine subjected to erosive/abrasive challenges.

Comparison of two vertical condensation obturation techniques: Touch 'n Heat modified and System B.

PubMed

Silver, G K; Love, R M; Purton, D G

1999-08-01

The aims of this study were firstly to compare the area of canal occupied by gutta-percha, sealer or voids using the System B heating device with that obtained by a modified vertical condensation technique using the Touch 'n Heat: and secondly to compare the temperature changes at the root canal wall and external root surface during obturation with the above techniques. Forty-five resin blocks, each with a standardized, simulated, prepared main root canal and five lateral canals, were assigned to three equal experimental groups. The canals were obturated using either the System B technique at two different temperature settings, or vertical condensation with a Touch 'n Heat instrument as the heat source. A heat transfer model was used to simultaneously record internal and external root surface temperature elevations during obturation by the three techniques. Data were analysed using unpaired Student's t-test and Mann-Whitney U-test. Both obturation techniques produced root fillings consisting of over 90% gutta-percha at most levels, although the percentages of sealer and voids 2-3 mm from the working length following System B obturation were higher than those found following modified vertical condensation. Modified vertical condensation resulted in more gutta-percha in lateral canals. Obturation was accomplished more quickly using the System B, and temperature elevations produced during obturation with the System B were significantly less (P < 0.001) than with vertical condensation. An elevation of external root surface temperature by more than 10 degrees C occurred during vertical condensation. The results suggest that the System B may produce an acceptable obturation and that the use of a Touch 'n Heat source during vertical condensation may result in damage to the periodontium.

Desirable plant root traits for protecting unstable slopes against landslides

NASA Astrophysics Data System (ADS)

Stokes, A.; Atger, C.; Bengough, G.; Fourcaud, T.; Sidle, R. C.

2009-04-01

A trait is defined as a distinct, quantitative property of organisms, usually measured at the individual level and used comparatively across species. Plant quantitative traits are extremely important for understanding the local ecology of any site. Plant height, architecture, root depth, wood density, leaf size and leaf nitrogen concentration control ecosystem processes and define habitat for other taxa. An engineer conjecturing as to how plant traits may directly influence physical processes occurring on sloping land just needs to consider how e.g. canopy architecture and litter properties influence the partitioning of rainfall among interception loss, infiltration and runoff. Plant traits not only influence abiotic processes occurring at a site, but also the habitat for animals and invertebrates. Depending on the goal of the landslide engineer, the immediate and long-term effects of plant traits in an environment must be considered if a site is to remain viable and ecologically successful. When vegetation is considered in models of slope stability, usually the only root parameters taken into consideration are tensile strength and root area ratio. Root system spatial structure is not considered, although the length, orientation and diameter of roots are recognized as being of importance. Thick roots act like soil nails on slopes, reinforcing soil in the same way that concrete is reinforced with steel rods. The spatial position of these thick roots also has an indirect effect on soil fixation in that the location of thin and fine roots will depend on the arrangement of thick roots. Thin and fine roots act in tension during failure on slopes and if they cross the slip surface, are largely responsible for reinforcing soil on slopes. Therefore, the most important trait to consider initially is rooting depth. To stabilize a slope against a shallow landslide, roots must cross the shear surface. The number and thickness of roots in this zone will therefore largely determine slope stability. Rooting depth is species dependent when soil conditions are not limiting and the number of horizontal lateral roots borne on the vertical roots usually changes with depth. Therefore, the number and orientation of roots that the shear surface intersects will change significantly with rooting depth for the same plant, even for magnitudes of only several cm. Similarly, depending on the geometry of the root system, the angle at which a root crosses the shear surface can also have an influence on its resistance to pullout and breakage. The angle at which a root emerges from the parent root is dependent on root type, depth and species (when soil conditions are not limiting). Due to the physiology of roots, a root branch can be initiated at any point along a parent root, but not necessarily emerge fully from the parent root. These traits, along with others including size, relative growth rate, regeneration strategies, wood structure and strength will be discussed with regard to their influence on slope stability. How each of these traits is influenced by soil conditions and plantation techniques is also of extreme importance to the landslide engineer. The presence of obstacles in the soil, as well as compaction, affects root length and branching pattern. Roots of many species of woody plants on shallow soils also tend to grow along fractures deep into the underlying bedrock which allows roots to locate supplies of nutrient and water rich pockets. Rooting depths of herbaceous species in water-limited environments are highly correlated with infiltration depth, but waterlogged soils can asphyxiate tree roots, resulting in shallow root systems. The need to understand and integrate each of these traits for a species is not easy. Therefore, we suggest a hierarchy whereby traits are considered in order of importance, along with how external factors influence their expression over time.

Scanning electron microscopic study of the lingual papillae in the arctic fox (Alopex lagopus L., 1758).

PubMed

Jackowiak, H; Godynicki, S; Skieresz-Szewczyk, K; Trzcielińska-Lorych, J

2009-10-01

This study aims to show the distribution and the three-dimensional structure of the lingual papillae in the arctic fox. The macro- and microscopic structure of the tongue and its lingual papillae was studied in 11 adult arctic foxes. Two types of mechanical papillae were distinguished on the dorsal surface of the tongue--filiform papillae and conical papillae. The gustatory papillae in the arctic fox are represented by fungiform, vallate and foliate papillae. The keratinized filiform papillae on the anterior part of tongue are composed of one big posterior process accompanied by 10-12 secondary anterior processes. The number of anterior processes of filiform papillae undergo a complete reduction within the area between the posterior part of the body of the tongue and area of the vallate papillae. The conical papillae cover the whole dorsal surface of the root of the tongue, including the lateral parts surrounding the area of the vallate papillae and the posterior part of the root. The size of the conical papillae increases towards the root of the tongue but their density decreases. In the arctic fox, there are three pairs of vallate papillae distributed on the plan of a triangle. The diameter of vallate papillae in each successive pair is bigger. The wall surrounding the body of the vallate papilla and its gustatory trench is composed of six to eight conical papillae joined at various degree. The foliate papillae on both margins of the tongue consist of seven to nine laminae.

Possible Tuff Cones In Isidis Planitia, Mars

NASA Astrophysics Data System (ADS)

Seabrook, A. M.; Rothery, D. A.; Bridges, J. C.; Wright, I. P.

The Beagle 2 lander of the ESA Mars Express mission will touch down on the martian surface in December 2003 to conduct a primarily exobiological mission. The landing site will be within Isidis Planitia, an 1100 km diameter impact basin. Isidis contains many sub-kilometre-sized cones. These can be found singly, in clusters, and in straight or arcuate chains extending many kilometres. In some areas of the basin these cones can occupy over 10% of the surface, with the most densely populated areas being in the older western half of the basin. There are few cones around the basin rim. There is also variation in the erosional state of the cones both across the basin, and within smaller areas, implying a range in time of formation for the cones. We currently favour a tuff cone origin as an explanation for these features. Tuff cones on Earth are rooted volcanic features formed at vents by the interaction between magma or magmatic heat and surface or near-surface water. Lava flows likely to be associated with at least some of the cones if they had a cinder cone (rooted eruptions at vents in a dry environment) origin are absent. This suggests the involvement of suffi- cient volatiles both to explosively fragment the erupting magma, and to cool the ejecta enough to prevent the formation of clastogenic flows. If our tuff cone interpretation is correct, this has implications for the presence, abundance and long-term persistence of sub-surface volatiles (water or carbon dioxide) on Mars. An understanding of the mechanism of formation of the Isidis cones will assist the characterisation of the basin in preparation for the landing of Beagle 2, by providing information about the history of volatiles and volcanism in the basin, and the processes that resulted in the surface we see today.

Comparative Study of Lunar Roughness from Multi - Source Data

NASA Astrophysics Data System (ADS)

Lou, Y.; Kang, Z.

2017-07-01

The lunar terrain can show its collision and volcanic history. The lunar surface roughness can give a deep indication of the effects of lunar surface magma, sedimentation and uplift. This paper aims to get different information from the roughness through different data sources. Besides introducing the classical Root-mean-square height method and Morphological Surface Roughness (MSR) algorithm, this paper takes the area of the Jurassic mountain uplift in the Sinus Iridum and the Plato Crater area as experimental areas. And then make the comparison and contrast of the lunar roughness derived from LRO's DEM and CE-2 DOM. The experimental results show that the roughness obtained by the traditional roughness calculation method reflect the ups and downs of the topography, while the results obtained by morphological surface roughness algorithm show the smoothness of the lunar surface. So, we can first use the surface fluctuation situation derived from RMSH to select the landing area range which ensures the lands are gentle. Then the morphological results determine whether the landing area is suitable for the detector walking and observing. The results obtained at two different scales provide a more complete evaluation system for selecting the landing site of the lunar probe.

Dynamics of organochlorine contaminants in surface water and in Myriophyllum aquaticum plants of the River Xanaes in central Argentina during the annual dry season.

PubMed

Schreiber, René; Harguinteguy, Carlos A; Manetti, Martin D

2013-10-01

The dynamics of organochlorine pesticides (OCPs) and their major metabolites were studied in surface waters and plants of the River Xanaes (province of Córdoba, Argentina) during the annual dry season. The results of the 5-month monitoring study (April to August 2010) showed similar low contamination levels in nonagricultural mountain and agricultural areas in both water and plants. The concentrations of compounds detected in the surface water were <4.5 ng L(-1), whereas concentrations of these substances in Myriophyllum aquaticum plants were <5 μg kg(-1) (dry weight) with the exception of trans-permethrin (17.6 μg kg(-1), dry weight). Because no notable differences in the contamination level between samples from the mountain and the agricultural area were observed, it was assumed that OCPs may not play an important role in today's pesticide use in this area. Furthermore, the concentration-time trends for OCPs in the submerged plants showed a generally similar elimination behaviour independent of compound and sampling site, thus indicating an integral rather then a substance-specific process, such as partitioning between the plant and the ambient water. As known, rooted macrophytes can take up contaminants by way of roots, so sediments may be the principal source. To understand the dynamics of these compounds in the river area more deeply, thus further research should include study of the river sediment.

Preparation of the apical part of the root canal by the Lightspeed and step-back techniques.

PubMed

Portenier, I; Lutz, F; Barbakow, F

1998-03-01

This study measured in vitro the displacement of natural canal centres in 18 human teeth before and after shaping by the step-back or Lightspeed techniques. Experimental roots (n = 9 per group), embedded in clear plastic, were cross-sectioned using a 0.1-mm-thick band saw at distances 1.25 mm, 3.25 mm and 5.25 mm from the apices. A stereo microscope was used to take 35 mm slides of the cut surfaces of the sectioned roots and canals. The slides of the uninstrumented canals were scanned into a computer and saved. Each sectioned root was then reassembled and the canals shaped by the step-back or Lightspeed technique. File size 40 and instrument size 50 were selected as the master apical file and master apical rotary for the step-back and Lightspeed groups, respectively. The 18 prepared canals were photographed, and the 35 mm slides scanned and computer stored as previously. This allowed the positions of the pre- and postinstrumented roots to be electronically superimposed for subsequent analyses. Displacements of the root canal centres before and after preparation were assessed in relation to the cross-sectional diameter of the files or instruments used. In addition, increases in cross-sectional area of the root canals after preparation were evaluated in relation to the cross-sectional area of the files or instruments used. Engine-driven nickel-titanium Lightspeed instruments caused significantly less (P < 0.001) displacement of the canal centres, so roots in the Lightspeed group remained better centred than those in the step-back group. The mean cross-sectional area after preparation in the Lightspeed group was significantly less (P < 0.001) than that recorded in the step-back group. Clinically, this implies less apical transportation and less dentine destruction with the Lightspeed technique than with the step-back technique.

Root carboxylate exudation capacity under phosphorus stress does not improve grain yield in green gram.

PubMed

Pandey, Renu; Meena, Surendra Kumar; Krishnapriya, Vengavasi; Ahmad, Altaf; Kishora, Naval

2014-06-01

Genetic variability in carboxylate exudation capacity along with improved root traits was a key mechanism for P-efficient green gram genotype to cope with P-stress but it did not increase grain yield. This study evaluates genotypic variability in green gram for total root carbon exudation under low phosphorus (P) using (14)C and its relationship with root exuded carboxylates, growth and yield potential in contrasting genotypes. Forty-four genotypes grown hydroponically with low (2 μM) and sufficient (100 μM) P concentrations were exposed to (14)CO2 to screen for total root carbon exudation. Contrasting genotypes were employed to study carboxylate exudation and their performance in soil at two P levels. Based on relative (14)C exudation and biomass, genotypes were categorized. Carboxylic acids were measured in exudates and root apices of contrasting genotypes belonging to efficient and inefficient categories. Oxalic and citric acids were released into the medium under low-P. PDM-139 (efficient) was highly efficient in carboxylate exudation as compared to ML-818 (inefficient). In low soil P, the reduction in biomass was higher in ML-818 as compared to PDM-139. Total leaf area and photosynthetic rate averaged for genotypes increased by 71 and 41 %, respectively, with P fertilization. Significantly, higher root surface area and volume were observed in PDM-139 under low soil P. Though the grain yield was higher in ML-818, the total plant biomass was significantly higher in PDM-139 indicating improved P uptake and its efficient translation into biomass. The higher carboxylate exudation capacity and improved root traits in the later genotype might be the possible adaptive mechanisms to cope with P-stress. However, it is not necessary that higher root exudation would result in higher grain yield.

Root surface removal and resultant surface texture with diamond-coated ultrasonic inserts: an in vitro and SEM study.

PubMed

Vastardis, Sotirios; Yukna, Raymond A; Rice, David A; Mercante, Don

2005-05-01

A new diamond-coated ultrasonic insert has been developed for scaling and root planing, and it was evaluated in vitro for the amount of root surface removed and the roughness of the residual root surface as a result of instrumentation. 48 extracted single-rooted human teeth were ground flat on one root surface and mounted (flat side up) in PVC rings of standard height and diameter with improved dental stone. Each tooth surface was treated with either a plain ultrasonic insert (PI), an ultrasonic insert with a fine grit diamond coating (DI) or sharp Gracey curettes (HI). The mounted teeth were attached to a stepper motor which drove the teeth in a horizontal, reciprocal motion at a constant rate. The thickness from the flattened bottom of the ring to the flattened tooth surface was measured before and after 10, 20, and 30 instrumentation strokes for each root surface with each of the experimental instruments. A number of treated teeth were randomly selected for examination with SEM and a profilometer. Statistical analysis (analysis of co-variance) was performed to compare the amounts of tooth structure removed among the 3 instruments and t-test was used to compare the roughness of the treated root surfaces. The mean depth of root structure removed was PI 10.7 microm, HI 15.0 microm, and DI 46.2 microm after 10 strokes; and PI 21.6 microm, HI 33.2 and DI 142.0 microm after 30 strokes, respectively. On average, 0.9 microm, 1.3 microm, and 4.7 microm of root surface was removed with each stroke of PI, HI and DI, respectively. PI and HI were not different from each other for all the stroke cycles, while DI was significantly different from PI and HI for all the stroke cycles (p<0.0001). Analysis with the profilometer showed that the smoothest surface was produced by the PI followed by the HI. The DI produced a surface that was significantly rougher than the surface produced by the PI or HI. These results suggest that diamond-coated ultrasonic instruments will effectively plane roots, and that caution should be used during periodontal root planing procedures. Additionally, the diamond-coated instruments will produce a rougher surface than the plain inserts or the hand curettes.

Incorporating Plant Phenology Dynamics in a Biophysical Canopy Model

NASA Technical Reports Server (NTRS)

Barata, Raquel A.; Drewry, Darren

2012-01-01

The Multi-Layer Canopy Model (MLCan) is a vegetation model created to capture plant responses to environmental change. Themodel vertically resolves carbon uptake, water vapor and energy exchange at each canopy level by coupling photosynthesis, stomatal conductance and leaf energy balance. The model is forced by incoming shortwave and longwave radiation, as well as near-surface meteorological conditions. The original formulation of MLCan utilized canopy structural traits derived from observations. This project aims to incorporate a plant phenology scheme within MLCan allowing these structural traits to vary dynamically. In the plant phenology scheme implemented here, plant growth is dependent on environmental conditions such as air temperature and soil moisture. The scheme includes functionality that models plant germination, growth, and senescence. These growth stages dictate the variation in six different vegetative carbon pools: storage, leaves, stem, coarse roots, fine roots, and reproductive. The magnitudes of these carbon pools determine land surface parameters such as leaf area index, canopy height, rooting depth and root water uptake capacity. Coupling this phenology scheme with MLCan allows for a more flexible representation of the structure and function of vegetation as it responds to changing environmental conditions.

Relationship of cariogenic bacteria levels with periodontal status and root surface caries in elderly Japanese.

PubMed

Saotome, Yasuhiko; Tada, Akio; Hanada, Nobuhiro; Yoshihara, Akihiro; Uematsu, Hiroshi; Miyazaki, Hideo; Senpuku, Hidenobu

2006-12-01

The relationship of the levels of cariogenic bacterial species with periodontal status and decayed root surfaces was investigated in elderly Japanese subjects. Three hundred and sixty-eight individuals (each 75 years old) were examined for periodontal status (pocket depth, attachment loss), root surface caries and salivary levels of mutans streptococci (MS) and lactobacilli (LB). Values >4 mm of attachment loss (rAL4) and for average attachment loss (aAL) of sites measured were significantly higher in subjects with LB than those without. Multiple regression analysis also showed a correlation between aAL and rAL4 values with the presence of LB (aAL p = 0.003; rAL4 p = 0.002). Further, multiple regression analysis of interacting factors regarding decayed root surfaces showed that LB carriers had a greater incidence of decayed root surface caries (p = 0.003), while MS and LB levels were correlated to the number of decayed root surfaces (LB p = 0.010; MS p = 0.026). Our results indicate that considerable attachment loss elevates the possibility of having LB, thus increasing the risk of root surface caries. It was also found that LB and MS measurements may be useful indicators of decayed root surfaces in elderly individuals with attachment loss.

Plant-Microbe Communication Enhances Auxin Biosynthesis by a Root-Associated Bacterium, Bacillus amyloliquefaciens SQR9.

PubMed

Liu, Yunpeng; Chen, Lin; Zhang, Nan; Li, Zunfeng; Zhang, Guishan; Xu, Yu; Shen, Qirong; Zhang, Ruifu

2016-04-01

Mechanisms by which beneficial rhizobacteria promote plant growth include tryptophan-dependent indole-3-acetic acid (IAA) synthesis. The abundance of tryptophan in the rhizosphere, however, may influence the level of benefit provided by IAA-producing rhizobacteria. This study examined the cucumber-Bacillus amyloliquefaciens SQR9 system and found that SQR9, a bacterium previously shown to enhance the growth of cucumber, increased root secretion of tryptophan by three- to fourfold. Using a split-root system, SQR9 colonization of roots in one chamber not only increased tryptophan secretion from the noninoculated roots but also increased the expression of the cucumber tryptophan transport gene but not the anthranilate synthesis gene in those roots. The increased tryptophan in isolated rhizosphere exudates was sufficient to support increased IAA production by SQR9. Moreover, SQR9 colonization of roots in one chamber in the split-root system resulted in sufficient tryptophan production by the other roots to upregulate SQR9 IAA biosynthesis genes, including a 27-fold increase in the indole-3-acetonitrilase gene yhcX during subsequent colonization of those roots. Deletion of yhcX eliminated SQR9-mediated increases in root surface area, likely by reducing IAA-stimulated lateral root growth. This study demonstrates a chemical dialogue between B. amyloliquefaciens and cucumber in which this communication contributes to bacteria-mediated plant-growth enhancement.

Effect of microcystins on root growth, oxidative response, and exudation of rice (Oryza sativa).

PubMed

Cao, Qing; Rediske, Richard R; Yao, Lei; Xie, Liqiang

2018-03-01

A 30 days indoor hydroponic experiment was carried out to evaluate the effect of microcystins (MCs) on rice root morphology and exudation, as well as bioaccumulation of MCs in rice. MCs were bioaccumulated in rice with the greatest concentrations being observed in the leaves (113.68μgg -1 Fresh weight (FW)) when exposed to 500μgL -1 MCs. Root activity at 500μgL -1 decreased 37%, compared to the control. MCs also induced disruption of the antioxidant system and lipid peroxidation in rice roots. Root growth was significantly inhibited by MCs. Root weight, length; surface area and volume were significantly decreased, as well as crown root number and lateral root number. After 30 days exposure to MCs, an increase was found in tartaric acid and malic acid while the other organic acids were not affected. Glycine, tyrosine, and glutamate were the only amino acids stimulated at MCs concentrations of 500μgL -1 . Similarly, dissolved organic carbon (DOC) and carbohydrate at 50 and 500μgL -1 treatments were significantly increased. The increase of DOC and carbohydrate in root exudates was due to rice root membrane permeability changes induced by MCs. Overall, this study indicated that MCs significantly inhibited rice root growth and affected root exudation. Copyright © 2017 Elsevier Inc. All rights reserved.

The effect of ultrasonic post instrumentation on root surface temperature.

PubMed

Huttula, Andrew S; Tordik, Patricia A; Imamura, Glen; Eichmiller, Frederick C; McClanahan, Scott B

2006-11-01

This study measured root surface temperature changes when ultrasonic vibration, with and without irrigation, was applied to cemented endodontic posts. Twenty-six, extracted, single-rooted premolars were randomly divided into two groups. Root lengths were standardized, canals instrumented, obturated, and posts cemented into prepared spaces. Thermocouples were positioned at two locations on the proximal root surfaces. Samples were embedded in plaster and brought to 37 degrees C in a water bath. Posts were ultrasonically vibrated for 4 minutes while continuously measuring temperature. Two-way ANOVA compared effects of water coolant and thermocouple location on temperature change. Root surface temperatures were significantly higher (p < 0.001) when posts were instrumented dry. A trend for higher temperatures was observed at coronal thermocouples of nonirrigated teeth and at apical thermocouples of irrigated teeth (p = 0.057). Irrigation during post removal with ultrasonics had a significant impact on the temperature measured at the external root surface.

Complex Apical Intraradicular Infection and Extraradicular Mineralized Biofilms as the Cause of Wet Canals and Treatment Failure: Report of 2 Cases.

PubMed

Ricucci, Domenico; Candeiro, George T M; Bugea, Calogero; Siqueira, José F

2016-03-01

This article describes 2 cases that showed persistent intracanal exudation (wet canal) even after several visits of antimicrobial endodontic treatment. Histologic and histobacteriologic investigation was conducted for determination of the cause. The 2 cases involved teeth with apical periodontitis lesions, which presented persistent exudation refractory to treatment after several visits. In case 1, it was not possible to achieve a dry canal, and surgery had to be performed. In case 2, attempts to dry the canal succeeded and the canal was filled, but follow-up examination showed an enlarged apical periodontitis lesion and extraction was performed. Biopsy specimens consisting of the root apex and apical periodontitis lesion for case 1 and the whole root for case 2 were subjected to histologic and histobacteriologic analyses. Both cases showed complex bacterial infection in the apical root, affecting both the intraradicular space and the outer root surface. Case 1 showed bacterial biofilms in ramifications, on untouched walls, and extending to the external root surface to form a thick and partially mineralized structure with high bacterial density. Different bacterial morphotypes were evidenced. Case 2 had a ledge on the apical canal wall created during instrumentation, which was filled with necrotic debris, filling material, and bacteria. The walls of the apical portion of the canal were covered by a bacterial biofilm, which was continuous with a thick extraradicular biofilm covering the cementum and dentin in resorptive defects. The extraradicular biofilm showed areas of mineralization and was dominated by filamentous bacteria. The 2 cases with wet canals and treatment failure were associated with complex persistent infection in the apical part of the root canal system extending to form thick and partially mineralized biofilm structures (calculus) on the outer apical root surface. Copyright © 2016 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Reference values of left heart echocardiographic dimensions and mass in male peri-pubertal athletes.

PubMed

Cavarretta, Elena; Maffessanti, Francesco; Sperandii, Fabio; Guerra, Emanuele; Quaranta, Federico; Nigro, Antonia; Minati, Monia; Rebecchi, Marco; Fossati, Chiara; Calò, Leonardo; Pigozzi, Fabio

2018-01-01

Background Several articles have proposed reference values in healthy paediatric subjects, but none of them has evaluated a large population of healthy trained adolescents. Design The study purpose was to establish normal echocardiographic measurements of left heart (aortic root, left atrium and left ventricular dimensions and mass) in relation to age, weight, height, body mass index, body surface area and training hours in this specific population. Methods We retrospectively evaluated 2151 consecutive, healthy, peri-pubertal athletes (100% male, mean age 12.4 ± 1.4 years, range 8-18) referred to a single centre for pre-participation screening. All participants were young soccer athletes who trained for a mean of 7.2 ± 1.1 h per week. Results Left ventricular internal diameters, wall thickness, left ventricular mass, aortic root and left atrium diameters were significantly correlated to age, body surface area, height and weight ( p < 0.01). Age, height, weight and body surface area were found associated with chamber size, while body mass index and training hours were not. Inclusion of both age and body size parameters in the statistical models resulted in improved overall explained variance for diameters and left ventricular mass. Conclusion Equations, mean values and percentile charts for the different age groups may be useful as reference data in efficiently assessing left ventricular parameters in young athletes.

Soil microbial biomass and root growth in Bt and non-Bt cotton

NASA Astrophysics Data System (ADS)

Tan, D. K. Y.; Broughton, K.; Knox, O. G.; Hulugalle, N. R.

2012-04-01

The introduction of transgenic Bacillus thuringiensis (Bt) cotton (Gossypium hirsutum L.) has had a substantial impact on pest management in the cotton industry. While there has been substantial research done on the impact of Bt on the above-ground parts of the cotton plant, less is known about the effect of Bt genes on below ground growth of cotton and soil microbial biomass. The aim of this research was to test the hypothesis that Bt [Sicot 80 BRF (Bollgard II Roundup Ready Flex®)] and non-Bt [Sicot 80 RRF (Roundup Ready Flex®)] transgenic cotton varieties differ in root growth and root turnover, carbon indices and microbial biomass. A field experiment was conducted in Narrabri, north-western NSW. The experimental layout was a randomised block design and used minirhizotron and core break and root washing methods to measure cotton root growth and turnover during the 2008/09 season. Root growth in the surface 0-0.1 m of the soil was measured using the core break and root washing methods, and that in the 0.1 to 1 m depth was measured with a minirhizotron and an I-CAP image capture system. These measurements were used to calculate root length per unit area, root carbon added to the soil through intra-seasonal root death, carbon in roots remaining at the end of the season and root carbon potentially added to the soil. Microbial biomass was also measured using the ninhydrin reactive N method. Root length densities and length per unit area of non-Bt cotton were greater than Bt cotton. There were no differences in root turnover between Bt and non-Bt cotton at 0-1 m soil depth, indicating that soil organic carbon stocks may not be affected by cotton variety. Cotton variety did not have an effect on soil microbial biomass. The results indicate that while there are differences in root morphology between Bt and non-Bt cotton, these do not change the carbon turnover dynamics in the soil.

X-Ray Microanalysis of Human Cementum

NASA Astrophysics Data System (ADS)

Alvarez-Pérez, Marco Antonio; Alvarez-Fregoso, Octavio; Ortiz-López, Jaime; Arzate, Higinio

2005-08-01

An energy dispersive x-ray microanalysis study was performed throughout the total length of cementum on five impacted human teeth. Mineral content of calcium, phosphorous, and magnesium were determined with an electron probe from the cemento-enamel junction to the root apex on the external surface of the cementum. The concentration profiles for calcium, phosphorous, and magnesium were compared by using Ca/P and Mg/Ca atomic percent ratio. Our findings demonstrated that the Ca/P ratio at the cemento-enamel junction showed the highest values (1.8 2.2). However, the area corresponding to the acellular extrinsic fiber cementum (AEFC) usually located on the coronal one-third of the root surface showed a Ca/P media value of 1.65. Nevertheless, on the area representing the fulcrum of the root there is an abrupt change in the Ca/P ratio, which decreases to 1.3. Our results revealed that Mg2+ distribution throughout the length of human cementum reached its maximum Mg/Ca ratio value of 1.3 1.4 at.% around the fulcrum of the root and an average value of 0.03%. A remarkable finding was that the Mg/Ca ratio pattern distribution showed that in the region where the Ca/P ratio showed a decreasing tendency, the Mg/Ca ratio reached its maximum value, showing a negative correlation. In conclusion, this study has established that clear compositional differences exist between AEFC and cellular mixed stratified cementum varieties and adds new knowledge about Mg2+ distribution and suggests its provocative role regulating human cementum metabolism.

[The genotypic diversity of oral Actinomyces naeslundii of root caries in aged people].

PubMed

Guo, Bin; Yang, Fan; Jia, Yue; Xia, Qian; Zhou, Xue-Dong

2010-12-01

To investigate the genotypic diversity of Actinomyces naeslundii in aged people and the relationship between the genotypes of Actinomyces naeslundii and root caries. According to the inclusion criteria, 20 aged people with root caries and 20 without root caries were chosen into two groups for this study. Two sites were chosen in subjects with root caries: One site was the exposed sound root surface, and the other site was the root caries. In subjects without root caries the sampling site of root surface was exposed. Bacteria were cultured and then identified. Repetitive extragenic palindromic sequence-based polymerase chain reaction (REP-PCR) was used to analyze the genotypic diversity of the Actinomyces naeslundii clinic isolates. 299 strains were isolated from the groups, 156 strains were chosen to analyze, belonged to 61 different genotypes. At the site of sound root surface in the subjects with root caries, there were 57 strains with 25 different patterns. At the site of root caries and of sound root surface in subjects without caries, there were 34 strains with 25 different patterns and 65 strains with 26 different patterns respectively. There was the genotypic diversity within Actinomyces naeslundii. There was significant difference in the genotypes in every individual site. Many different genotypes of Actinomyces naeslundii concerned with occurrence of root caries.

Estimation of runoff mitigation by morphologically different cover crop root systems

NASA Astrophysics Data System (ADS)

Yu, Yang; Loiskandl, Willibald; Kaul, Hans-Peter; Himmelbauer, Margarita; Wei, Wei; Chen, Liding; Bodner, Gernot

2016-07-01

Hydrology is a major driver of biogeochemical processes underlying the distinct productivity of different biomes, including agricultural plantations. Understanding factors governing water fluxes in soil is therefore a key target for hydrological management. Our aim was to investigate changes in soil hydraulic conductivity driven by morphologically different root systems of cover crops and their impact on surface runoff. Root systems of twelve cover crop species were characterized and the corresponding hydraulic conductivity was measured by tension infiltrometry. Relations of root traits to Gardner's hydraulic conductivity function were determined and the impact on surface runoff was estimated using HYDRUS 2D. The species differed in both rooting density and root axes thickness, with legumes distinguished by coarser axes. Soil hydraulic conductivity was changed particularly in the plant row where roots are concentrated. Specific root length and median root radius were the best predictors for hydraulic conductivity changes. For an intensive rainfall simulation scenario up to 17% less rainfall was lost by surface runoff in case of the coarsely rooted legumes Melilotus officinalis and Lathyrus sativus, and the densely rooted Linum usitatissimum. Cover crops with coarse root axes and high rooting density enhance soil hydraulic conductivity and effectively reduce surface runoff. An appropriate functional root description can contribute to targeted cover crop selection for efficient runoff mitigation.

What can we learn from in-soil imaging of a live plant: X-ray Computed Tomography and 3D numerical simulation of root-soil system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, Xiaofan; Varga, Tamas; Liu, Chongxuan

Plant roots play a critical role in plant-soil-microbe interactions that occur in the rhizosphere, as well as processes with important implications to farming, forest management and climate change. X-ray computed tomography (XCT) has been proven to be an effective tool for non-invasive root imaging and analysis. A combination of XCT, open-source software, and our own code was used to noninvasively image a prairie dropseed (Sporobolus heterolepis) specimen, segment the root data to obtain a 3D image of the root structure at 31µm resolution, and extract quantitative information (root volume and surface area) from the 3D data, respectively. Based on themore » mesh generated from the root structure, computational fluid dynamics (CFD) simulations were applied to numerically investigate the root-soil-groundwater system. The plant root conductivity, soil hydraulic conductivity and transpiration rate were shown to control the groundwater distribution. The flow variability and soil water distributions under different scenarios were investigated. Parameterizations were evaluated to show their impacts on the average conductivity. The pore-scale modeling approach provides realistic simulations of rhizosphere flow processes and provides useful information that can be linked to upscaled models.« less

Water uptake by seminal and adventitious roots in relation to whole-plant water flow in barley (Hordeum vulgare L.)

PubMed Central

Knipfer, Thorsten; Fricke, Wieland

2011-01-01

Prior to an assessment of the role of aquaporins in root water uptake, the main path of water movement in different types of root and driving forces during day and night need to be known. In the present study on hydroponically grown barley (Hordeum vulgare L.) the two main root types of 14- to 17-d-old plants were analysed for hydraulic conductivity in dependence of the main driving force (hydrostatic, osmotic). Seminal roots contributed 92% and adventitious roots 8% to plant water uptake. The lower contribution of adventitious compared with seminal roots was associated with a smaller surface area and number of roots per plant and a lower axial hydraulic conductance, and occurred despite a less-developed endodermis. The radial hydraulic conductivity of the two types of root was similar and depended little on the prevailing driving force, suggesting that water uptake occurred along a pathway that involved crossing of membrane(s). Exudation experiments showed that osmotic forces were sufficient to support night-time transpiration, yet transpiration experiments and cuticle permeance data questioned the significance of osmotic forces. During the day, 90% of water uptake was driven by a tension of about –0.15 MPa. PMID:20974734

Effects of phosphorus supply on growth, phosphate concentration and cluster-root formation in three Lupinus species

PubMed Central

Abdolzadeh, Ahmad; Wang, Xing; Veneklaas, Erik J.; Lambers, Hans

2010-01-01

Background and Aims In some lupin species, phosphate deficiency induces cluster-root formation, which enhances P uptake by increasing root surface area and, more importantly, the release of root exudates which enhances P availability. Methods Three species of Lupinus, L. albus, L. atlanticus and L. micranthus, with inherently different relative growth rates were cultivated under hydroponics in a greenhouse at four phosphate concentrations (1, 10, 50 and 150 µm) to compare the role of internal P in regulating cluster-root formation. Key Results The highest growth rate was observed in L. atlanticus, followed by L. albus and L. micranthus. At 1 µm P, cluster-root formation was markedly induced in all three species. The highest P uptake and accumulation was observed in L. micranthus, followed by L. atlanticus and then L. albus. Inhibition of cluster-root formation was severe at 10 µm P in L. atlanticus, but occurred stepwise with increasing P concentration in the root medium in L. albus. Conclusions In L. atlanticus and L. albus cluster-root formation was suppressed by P treatments above 10 µm, indicating a P-inducible regulating system for cluster-root formation, as expected. By contrast, production of cluster roots in L. micranthus, in spite of a high internal P concentration, indicated a lower sensitivity to P status, which allowed P-toxicity symptoms to develop. PMID:20037142

Root Morphology Was Improved in a Late-Stage Vigor Super Rice Cultivar.

PubMed

Huang, Min; Chen, Jiana; Cao, Fangbo; Jiang, Ligeng; Zou, Yingbin

2015-01-01

This study aimed to test the hypothesis that root morphology might be improved and consequently contributing to superior post-heading shoot growth and grain yield in late-stage vigor super rice. A pot experiment was carried out to compare yield attributes, shoot growth and physiological properties and root morphological traits between a late-stage vigor super rice cultivar (Y-liangyou 087) and an elite rice cultivar (Teyou 838). Grain yield and total shoot biomass were 7-9% higher in Y-liangyou 087 than in Teyou 838. Y-liangyou 087 had 60-64% higher post-heading shoot growth rate and biomass production than Teyou 838. Average relative chlorophyll concentration and net photosynthetic rate in flag leaves were 7-11% higher in Y-liangyou 087 than in Teyou 838 during heading to 25 days after heading. Y-liangyou 087 had 41% higher post-heading shoot N uptake but 17-25% lower root biomass and root-shoot ratio at heading and maturity than Teyou 838. Specific root length and length and surface area of fine roots were higher in Y-liangyou 087 than in Teyou 838 at heading and maturity by more than 15%. These results indicated that root-shoot relationships were well balanced during post-heading phase in the late-stage vigor super rice cultivar Y-liangyou 087 by improving root morphology including avoiding a too great root biomass and developing a large fine root system.

Effect of organic matter and roots in soil respiration in a Mediterranean riparian areas in Central Spain

NASA Astrophysics Data System (ADS)

Gonzalez-Garrido, Laura; Delgado, Juan Antonio; Martinez, Teodora

2010-05-01

Soil respiration is one of the largest carbon flux components within terrestrial ecosystems, and small changes in the magnitude of soil respiration could have a large effect on the concentration of CO2 in the atmosphere. The main objective is evaluating the factors controlling soil respiration on the global carbon cycle in riparian areas of Henares River. We evaluated total soil respiration as it was affected by soil temperature, soil moisture, root respiration and organic matter in four areas differing in vegetation cover. We specifically assessed the contribution of soil organic matter and fine root biomass (≤1 mm.) in soil carbon dioxide flux. The study area is located on the riverbanks of Henares River where it passes through the municipal term of Alcala de Henares (Madrid) in Central Spain. Measurements were performed in spring and autumn of 2009. The study was conducted on four different types of riparian vegetation: natural Mediterranean riparian forest, reforestation of 1994, reforestation of 1999 and riparian grassland without trees. In each area of study 3, 25x25 m, plots were delimited and within each plot three sampling units of 50x50 cm were selected at random. The temperature of the ground was taken during the measures from respiration using a Multi-thermometer (-50°C - +300°C) at 5 cm depth. The moisture content of the ground was measured at 5 cm of depth with a HH2 Moisture meter (Delta Devices, Cambridge, UK). The measures of respiration of the ground were realised in field by means of LCI portable (LC pro ADC Bioscientific, Ltd. UK) connected to a ground respiration camera. We introduced the camera 3 cm into the soil just after eliminating the vegetation grass of the surface of measurement cutting carefully the aerial part, without damaging the roots. Soil CO2 flux measurements were registered after stabilization. Immediately after CO2 measurements, we obtained soil samples by means of a drill of 2.18 cm of diameter taking samples to 10 cm and 20 cm depth. Soil samples were dried to the air with the aim of preserving the roots the sample contained. They were extracted manually by means of very fine tweezers. We separate roots by diameter (Fine roots ≤ 1mm; rest of roots > 1mm) and dead from alive using texture and colour as clues. Finally the dry weight of roots was taking with a precision balance +-0.0001. Soil organic matter to 10 and 20 cm of depth were measure in laboratory using the method of Walkley and Black (1934). Differences in Soil CO2 flux, organic matter, fine root biomass, temperature and moisture between areas were analyzed using one-way ANOVAs. Our results suggest that fine root biomass present a larger impact than soil organic matter in soil CO2 flux values. Natural riparian forest presented higher values of soil CO2 flux than the rest of areas even when differences in root biomass and soil organic matter were controlled. Between the grassy area and both reforestations there were no differences in soil CO2 flux. In addition, we found that soil CO2 flux in our study area was more affected by soil temperature than by moisture, which could be relevant in the interpretation of the possible effects of global change. Key words: riparian forest, fine roots, carbon cycle, soil CO2 flux, root respiration. Acknowledgements: Research projects, n°FP08-AG02 IMIDRA and RTA 2006-00101-00-00 INIA and predoctoral scholarship FPI-INIA.

Characterizing roots and water uptake in a ground cover rice production system.

PubMed

Li, Sen; Zuo, Qiang; Wang, Xiaoyu; Ma, Wenwen; Jin, Xinxin; Shi, Jianchu; Ben-Gal, Alon

2017-01-01

Water-saving ground cover rice production systems (GCRPS) are gaining popularity in many parts of the world. We aimed to describe the characteristics of root growth, morphology, distribution, and water uptake for a GCRPS. A traditional paddy rice production system (TPRPS) was compared with GCRPS in greenhouse and field experiments. In the greenhouse, GCRPS where root zone average soil water content was kept near saturation (GCRPSsat), field capacity (GCRPSfwc) and 80% field capacity (GCRPS80%), were evaluated. In a two-year field experiment, GCRPSsat and GCRPS80% were applied. Similar results were found in greenhouse and field experiments. Before mid-tillering the upper soil temperature was higher for GCRPS, leading to enhanced root dry weight, length, surface area, specific root length, and smaller diameter of roots but lower water uptake rate per root length compared to TPRPS. In subsequent growth stages, the reduced soil water content under GCRPS caused that the preponderance of root growth under GCRPSsat disappeared in comparison to TPRPS. Under other GCRPS treatments (GCRPSfwc and GCRPS80%), significant limitation on root growth, bigger root diameter and higher water uptake rate per root length were found. Discrepancies in soil water and temperature between TPRPS and GCRPS caused adjustments to root growth, morphology, distribution and function. Even though drought stress was inevitable after mid-tillering under GCRPS, especially GCRPS80%, similar or even enhanced root water uptake capacity in comparison to TPRPS might promote allocation of photosynthetic products to shoots and increase water productivity.

A morphometric analysis of the redistribution of organelles in columella cells of horizontally-oriented roots of Zea mays

NASA Technical Reports Server (NTRS)

Moore, R.

1986-01-01

In order to determine what structural changes in graviperceptive cells are associated with onset of root gravicurvature, the redistribution of organelles in columella cells of horizontally-oriented, graviresponding roots of Zea mays has been quantified. Root gravicurvature began by 15 min after reorientation, and did not involve significant changes in the (i) volume of individual columella cells or amyloplasts, (ii) relative volume of any cellular organelle, (iii) number of amyloplasts per columella cell, or (iv) surface area of cellular location of endoplasmic reticulum. Sedimentation of amyloplasts began within 1 to 2 min after reorientation, and was characterized by an intensely staining area of cytoplasm adjacent to the sedimenting amyloplasts. By 5 min after reorientation, amyloplasts were located in the lower distal corner of columella cells, and, by 15 min after reorientation, overlaid the entire length of the lower cell wall. No consistent contact between amyloplasts and any cellular structure was detected at any stage of gravicurvature. Centrally-located nuclei initially migrated upward in columella cells of horizontally-oriented roots, after which they moved to the proximal ends of the cells by 15 min after reorientation. No significant pattern of redistribution of vacuoles, mitochondria, dictyosomes, or hyaloplasm was detected that correlated with the onset of gravicurvature. These results indicate that amyloplasts and nuclei are the only organelles whose movements correlate positively with the onset of gravicurvature by primary roots of this cultivar of Zea mays.

a New Method for Calculating the Fractal Dimension of Surface Topography

NASA Astrophysics Data System (ADS)

Zuo, Xue; Zhu, Hua; Zhou, Yuankai; Li, Yan

2015-06-01

A new method termed as three-dimensional root-mean-square (3D-RMS) method, is proposed to calculate the fractal dimension (FD) of machined surfaces. The measure of this method is the root-mean-square value of surface data, and the scale is the side length of square in the projection plane. In order to evaluate the calculation accuracy of the proposed method, the isotropic surfaces with deterministic FD are generated based on the fractional Brownian function and Weierstrass-Mandelbrot (WM) fractal function, and two kinds of anisotropic surfaces are generated by stretching or rotating a WM fractal curve. Their FDs are estimated by the proposed method, as well as differential boxing-counting (DBC) method, triangular prism surface area (TPSA) method and variation method (VM). The results show that the 3D-RMS method performs better than the other methods with a lower relative error for both isotropic and anisotropic surfaces, especially for the surfaces with dimensions higher than 2.5, since the relative error between the estimated value and its theoretical value decreases with theoretical FD. Finally, the electrodeposited surface, end-turning surface and grinding surface are chosen as examples to illustrate the application of 3D-RMS method on the real machined surfaces. This method gives a new way to accurately calculate the FD from the surface topographic data.

Emergent macrophytes select for nitrifying and denitrifying microorganisms in constructed wetlands

NASA Astrophysics Data System (ADS)

Trias, Rosalia; Ramió Pujol, Sara; Bañeras, Lluis

2014-05-01

The use of constructed wetlands for wastewater treatment is a reliable low-cost alternative that has been widely developed during the last years. Several processes involving plants, sediments, and microbial communities contribute to nitrogen removal in wetlands. Vegetation plays an important role in this process, not only by nutrient assimilation but also by the stimulation of the plant associated microbiota. Plants supply oxygen at the close proximity of the root surface that may favour ammonia oxidizers. At the same time, exudation of organic compounds potentially speeds-up denitrification in the anoxic environment. The aim of this work was to understand the plant-microbe interactions at the root level in the Empuriabrava free water surface constructed wetland (Spain). The roots of the macrophytes Typha latifolia, Typha angustifolia, Phragmites australis and Bolboschoenus maritimus were sampled at four dates from January to September 2012, covering all the stages of plant growth. Additionally, sediment surrounding vegetation and non-vegetated sediments were sampled. Microbial community structure was analysed by pyrosequencing of bacterial and archaeal 16S rDNA and functional genes (nirK, nirS, nosZ and amoA). Bacterial communities were significantly different in sediments of the vegetated areas compared to the root surface. Plant roots exhibited a higher proportion of proteobacteria whereas Actinobacteria were dominant in sediments. The nitrifiers Nitrosomonas sp. and Nitrosococcus sp. accounted for less than 1% of all sequences. Archaeal communities were dominated by the Miscellaneous Crenarchaeotic Groups C2 and C3 and Methanomicrobia. Higher relative abundances of MCG were found in roots of P. australis, B. maritimus and T. angustifolia. Ammonia oxidizing archaea accounted for less than 0.1% of all sequences but were consistently more abundant in sediment samples compared to roots. NirK and NirS-type bacterial communities showed clearly distinct distribution patterns among plant species, thus indicating different plant-microbe relationships for the two bacterial groups. Our results show that plant roots have implications in multiple steps of the nitrogen cycle and can significantly alter nitrogen removal in wetlands.

[Arbuscular mycorrhizal symbiosis influences the biological effects of nano-ZnO on maize].

PubMed

Wang, Wei-Zhong; Wang, Fa-Yuan; Li, Shuai; Liu, Xue-Qin

2014-08-01

Engineered nanoparticles (ENPs) can be taken up and accumulated in plants, then enter human bodies via food chain, and thus cause potential health risk. Arbuscular mycorrhizal fungi form mutualistic symbioses with the majority of higher plants in terrestrial ecosystems, and potentially influence the biological effects of ENPs. The present greenhouse pot culture experiment studied the effects of inoculation with or without arbuscular mycorrhizal fungus Acaulospora mellea on growth and nutritional status of maize under different nano-ZnO levels (0, 500, 1 000, 2000 and 3 000 mg x kg(-1)) artificially added into soil. Results showed that with the increasing nano-ZnO levels in soil, mycorrhizal colonization rate and biomass of maize plants showed a decreasing trend, total root length, total surface area and total volume reduced, while Zn concentration and uptake in plants gradually increased, and P, N, K, Fe, and Cu uptake in shoots all decreased. Compared with the controls, arbuscular mycorrhizal inoculation improved the growth and P, N and K nutrition of maize, enhanced total root length, total surface area and total volume, and increased Zn allocation to roots when nano-ZnO was added. Our results firstly show that nano-ZnO in soil induces toxicity to arbuscular mycorrhizae, while arbuscular mycorrhizal inoculation can alleviate its toxicity and play a protective role in plants.

Understanding genetic control of root system architecture in soybean: Insights into the genetic basis of lateral root number.

PubMed

Prince, Silvas J; Valliyodan, Babu; Ye, Heng; Yang, Ming; Tai, Shuaishuai; Hu, Wushu; Murphy, Mackensie; Durnell, Lorellin A; Song, Li; Joshi, Trupti; Liu, Yang; Van de Velde, Jan; Vandepoele, Klaas; Grover Shannon, J; Nguyen, Henry T

2018-05-10

Developing crops with better root systems is a promising strategy to ensure productivity in both optimum and stress environments. Root system architectural (RSA) traits in 397 soybean accessions were characterized and a high-density single nucleotide polymorphisms (SNP) based genome-wide association study was performed to identify the underlying genes associated with root structure. SNPs associated with root architectural traits specific to landraces and elite germplasm pools were detected. Four loci were detected in landraces for lateral root number (LRN) and distribution of root thickness in diameter class I with a major locus on chromosome 16. This major loci was detected in the coding region of unknown protein, and subsequent analyses demonstrated that root traits are affected with mutated haplotypes of the gene. In elite germplasm pool, three significant SNPs in alanine-glyoxalate aminotransferase, Leucine-Rich Repeat receptor/No apical meristem and unknown functional genes were found to govern multiple traits including root surface area and volume. However, no major loci were detected for LRN in elite germplasm. Nucleotide diversity analysis found evidence of selective sweeps around the landraces LRN gene. Soybean accessions with minor and mutated allelic variants of LRN gene were found to perform better in both water-limited and optimal field conditions. This article is protected by copyright. All rights reserved.

Potential for post-closure radionuclide redistribution due to biotic intrusion: aboveground biomass, litter production rates, and the distribution of root mass with depth at material disposal area G, Los Alamos National Laboratory

DOE Office of Scientific and Technical Information (OSTI.GOV)

French, Sean B; Christensen, Candace; Jennings, Terry L

2008-01-01

Low-level radioactive waste (LLW) generated at the Los Alamos National Laboratories (LANL) is disposed of at LANL's Technical Area (T A) 54, Material Disposal Area (MDA) G. The ability of MDA G to safely contain radioactive waste during current and post-closure operations is evaluated as part of the facility's ongoing performance assessment (PA) and composite analysis (CA). Due to the potential for uptake and incorporation of radio nuclides into aboveground plant material, the PA and CA project that plant roots penetrating into buried waste may lead to releases of radionuclides into the accessible environment. The potential amount ofcontamination deposited onmore » the ground surface due to plant intrusion into buried waste is a function of the quantity of litter generated by plants, as well as radionuclide concentrations within the litter. Radionuclide concentrations in plant litter is dependent on the distribution of root mass with depth and the efficiency with which radionuclides are extracted from contaminated soils by the plant's roots. In order to reduce uncertainties associated with the PA and CA for MDA G, surveys are being conducted to assess aboveground biomass, plant litter production rates, and root mass with depth for the four prominent vegetation types (grasses, forbs, shrubs and trees). The collection of aboveground biomass for grasses and forbs began in 2007. Additional sampling was conducted in October 2008 to measure root mass with depth and to collect additional aboveground biomass data for the types of grasses, forbs, shrubs, and trees that may become established at MDA G after the facility undergoes final closure, Biomass data will be used to estimate the future potential mass of contaminated plant litter fall, which could act as a latent conduit for radionuclide transport from the closed disposal area. Data collected are expected to reduce uncertainties associated with the PA and CA for MDA G and ultimately aid in the assessment and subsequent prevention of radionuclide transport within the environment from the closed disposal area and potential exposure to site workers and the public.« less

Influence of arbuscular mycorrhizae on the root system of maize plants under salt stress.

PubMed

Sheng, Min; Tang, Ming; Chen, Hui; Yang, Baowei; Zhang, Fengfeng; Huang, Yanhui

2009-07-01

Salt stress has become a severe global problem, and salinity is one of the most important abiotic factors limiting plant growth and yield. It is known that arbuscular mycorrhizal (AM) fungi decrease plant yield losses under salinity. With the aim of determining whether AM inoculation would give an advantage to root development under salt stress, a greenhouse experiment was carried out with AM or without AM fungi. Maize plants were grown in a sand and soil mixture with 5 NaCl levels (0, 0.5, 1.0, 1.5, and 2.0 g/kg dry substrate) for 55 days, following 15 days of nonsaline pretreatment. At all salt levels, mycorrhizal plants had higher dry shoot and root mass, higher root activity, and lower root to shoot ratios than non-mycorrhizal plants. In salt-free soil, root length, root surface area, root volume, and number of root tips and forks were significantly larger in mycorrhizal plants than in non-mycorrhizal plants, whereas, under salt stress, average root diameter and root volume of mycorrhizal plants were larger than those of non-mycorrhizal plants. Regardless of the NaCl level, mycorrhizal plants had lower specific root length, lower percentage of root length in the 0-0.2 mm diameter class, and higher percentage of root length in both the 0.2-0.4 mm and 0.4-0.6 mm diameter classes, which suggests that the root system shows a significant shift towards a thicker root system when maize plants were inoculated with Glomus mosseae (Nicolson & Gerdemann). The results presented here indicate that the improvements in root activity and the coarse root system of mycorrhizal maize may help in alleviating salt stress on the plant.

Quantitative Phosphoproteomic Analysis of Soybean Root Hairs Inoculated with Bradyrhizobium japonicum

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nguyen, Tran H.; Brechenmacher, Laurent; Aldrich, Joshua T.

2012-11-11

Root hairs are single hair-forming cells on roots that function to increase root surface area, enhancing water and nutrient uptake. In leguminous plants, root hairs also play a critical role as the site of infection by symbiotic nitrogen fixing rhizobia, leading to the formation of a novel organ, the nodule. The initial steps in the rhizobia-root hair infection process are known to involve specific receptor kinases and subsequent kinase cascades. Here, we characterize the phosphoproteome of the root hairs and the corresponding stripped roots (i.e., roots from which root hairs were removed) during rhizobial colonization and infection to gain insightmore » into the molecular mechanism of root hair cell biology. We chose soybean (Glycine max L.), one of the most important crop plants in the legume family, for this study because of its larger root size, which permits isolation of sufficient root hair material for phosphoproteomic analysis. Phosphopeptides derived from root hairs and stripped roots, mock inoculated or inoculated with the soybean-specific rhizobium Bradyrhizobium japonicum, were labeled with the isobaric tag 8-plex ITRAQ, enriched using Ni-NTA magnetic beads and subjected to nRPLC-MS/MS analysis using HCD and decision tree guided CID/ETD strategy. A total of 1,625 unique phosphopeptides, spanning 1,659 non-redundant phosphorylation sites, were detected from 1,126 soybean phosphoproteins. Among them, 273 phosphopeptides corresponding to 240 phosphoproteins were found to be significantly regulated (>1.5 fold abundance change) in response to inoculation with B. japonicum. The data reveal unique features of the soybean root hair phosphoproteome, including root hair and stripped root-specific phosphorylation suggesting a complex network of kinase-substrate and phosphatase-substrate interactions in response to rhizobial inoculation.« less

Application of Data Assimilation with the Root Zone Water Quality Model for Soil Moisture Profile Estimation

USDA-ARS?s Scientific Manuscript database

Estimation of soil moisture has received considerable attention in the areas of hydrology, agriculture, meteorology and environmental studies because of its role in the partitioning water and energy at the land surface. In this study, the Ensemble Kalman Filter (EnKF), a popular data assimilation te...

Tree Age Effects on Fine Root Biomass and Morphology over Chronosequences of Fagus sylvatica, Quercus robur and Alnus glutinosa Stands

PubMed Central

Jagodzinski, Andrzej M.; Ziółkowski, Jędrzej; Warnkowska, Aleksandra; Prais, Hubert

2016-01-01

There are few data on fine root biomass and morphology change in relation to stand age. Based on chronosequences for beech (9–140 years old), oak (11–140 years) and alder (4–76 years old) we aimed to examine how stand age affects fine root biomass and morphology. Soil cores from depths of 0–15 cm and 16–30 cm were used for the study. In contrast to previously published studies that suggested that maximum fine root biomass is reached at the canopy closure stage of stand development, we found almost linear increases of fine root biomass over stand age within the chronosequences. We did not observe any fine root biomass peak in the canopy closure stage. However, we found statistically significant increases of mean fine root biomass for the average individual tree in each chronosequence. Mean fine root biomass (0–30 cm) differed significantly among tree species chronosequences studied and was 4.32 Mg ha-1, 3.71 Mg ha-1 and 1.53 Mg ha-1, for beech, oak and alder stands, respectively. The highest fine root length, surface area, volume and number of fine root tips (0–30 cm soil depth), expressed on a stand area basis, occurred in beech stands, with medium values for oak stands and the lowest for alder stands. In the alder chronosequence all these values increased with stand age, in the beech chronosequence they decreased and in the oak chronosequence they increased until ca. 50 year old stands and then reached steady-state. Our study has proved statistically significant negative relationships between stand age and specific root length (SRL) in 0–30 cm soil depth for beech and oak chronosequences. Mean SRLs for each chronosequence were not significantly different among species for either soil depth studied. The results of this study indicate high fine root plasticity. Although only limited datasets are currently available, these data have provided valuable insight into fine root biomass and morphology of beech, oak and alder stands. PMID:26859755

Who's on first? Part I: Influence of plant growth on C association with fresh soil minerals

NASA Astrophysics Data System (ADS)

Neurath, R.; Whitman, T.; Nico, P. S.; Pett-Ridge, J.; Firestone, M. K.

2015-12-01

Mineral surfaces provide sites for carbon stabilization in soils, protecting soil organic matter (SOM) from microbial degradation. SOM distributed across mineral surfaces is expected to be patchy and certain minerals undergo re-mineralization under dynamic soil conditions, such that soil minerals surfaces can range from fresh to thickly-coated with SOM. Our research investigates the intersection of microbiology and geochemistry, and aims to build a mechanistic understanding of plant-derived carbon (C) association with mineral surfaces and the factors that determine SOM fate in soil. Plants are the primary source of C in soil, with roots exuding low-molecular weight compounds during growth and contributing more complex litter compounds at senescence. We grew the annual grass, Avena barbata, (wild oat) in a 99 atom% 13CO2 atmosphere in soil microcosms incubated with three mineral types representing a spectrum of reactivity and surface area: quartz, kaolinite, and ferrihydrite. These minerals, isolated in mesh bags to exclude roots but not microorganisms, were extracted and analyzed for total C and 13C at multiple plant growth stages. At plant senescence, the quartz had the least mineral-bound C (0.40 mg-g-1) and ferrihydrite the most (0.78 mg-g-1). Ferrihydrite and kaolinite also accumulated more plant-derived C (3.0 and 3.1% 13C, respectively). The experiment was repeated with partially digested 13C-labled root litter to simulate litter decomposition during plant senescence. Thus, we are able evaluate contributions derived from living and dead root materials on soil minerals using FTIR and 13C-NMR. We find that mineral-associated C bears a distinct microbial signature, with soil microbes not only transforming SOM prior to mineral association, but also populating mineral surfaces over time. Our research shows that both soil mineralogy and the chemical character of plant-derived compounds are important controls of mineral protection of SOM.

Microtensile bond strength of etch-and-rinse and self-etching adhesives to intrapulpal dentin after endodontic irrigation and setting of root canal sealer.

PubMed

Wattanawongpitak, Nipaporn; Nakajima, Masatoshi; Ikeda, Masaomi; Foxton, Richard M; Tagami, Junji

2009-02-01

To evaluate the effect of endodontic irrigation regimens and calcium hydroxide root canal sealer (Sealapex) on the microtensile bond strengths (muTBS) of dual-curing resin composite (Clearfil DC Core Automix) to the intrapulpal dentin. Forty standardized coronal-half root canal dentin specimens obtained from human premolars were divided into 4 groups: group A, no treatment (control); group B, Sealapex; group C, NaOCl/Sealapex; group D, EDTA/NaOCl/Sealapex. After 7 days of storage in 100% relative humidity, Sealapex was removed. Dentin surfaces were bonded with adhesives, either etch-and-rinse (Single Bond) or self-etching (Clearfil SE Bond), and built up with resin composite. The bonded specimens were trimmed into an hourglass shape with a 1-mm2 cross-sectional area for microtensile testing (n = 20). The muTBS to intrapulpal dentin was analyzed using two-way ANOVA and Dunnett's TC test. Two teeth of each group were prepared for micromorphological analysis of dentin surface. The root canal sealer with or without endodontic irrigation significantly affected the bond strengths of resin composite to intrapulpal dentin compared with the control group (p < 0.05). There were no significant differences in muTBS of each experimental group between etch-and-rinse and self-etching adhesives (p > 0.05). The dentin surface was covered with a mud-like material after sealer application for 7 days. The root canal sealer reduced the muTBS of dual-curing resin composite with etch-and-rinse and self-etching adhesive systems to intrapulpal dentin. Treatment with EDTA followed by NaOCI prior to obturation caused an additional reduction in muTBS of both adhesive systems to intrapulpal dentin.

A nonpathogenic Fusarium oxysporum strain enhances phytoextraction of heavy metals by the hyperaccumulator Sedum alfredii Hance.

PubMed

Zhang, Xincheng; Lin, Li; Chen, Mingyue; Zhu, Zhiqiang; Yang, Weidong; Chen, Bao; Yang, Xiaoe; An, Qianli

2012-08-30

Low biomass and shallow root systems limit the application of heavy metal phytoextraction by hyperaccumulators. Plant growth-promoting microbes may enhance hyperaccumulators'phytoextraction. A heavy metal-resistant fungus belonged to the Fusarium oxysporum complex was isolated from the Zn/Cd co-hyperaccumulator Sedum alfredii Hance grown in a Pb/Zn mined area. This Fusarium fungus was not pathogenic to plants but promoted host growth. Hydroponic experiments showed that 500 μM Zn(2+) or 50 μM Cd(2+) combined with the fungus increased root length, branches, and surface areas, enhanced nutrient uptake and chlorophyll synthesis, leading to more vigorous hyperaccumulators with greater root systems. Soil experiments showed that the fungus increased root and shoot biomass and S. alfredii-mediated heavy metal availabilities, uptake, translocation or concentrations, and thus increased phytoextraction of Zn (144% and 44%), Cd (139% and 55%), Pb (84% and 85%) and Cu (63% and 77%) from the original Pb/Zn mined soil and a multi-metal contaminated paddy soil. Together, the nonpathogenic Fusarium fungus was able to increase S. alfredii root systems and function, metal availability and accumulation, plant biomass, and thus phytoextraction efficiency. This study showed a great application potential for culturable indigenous fungi other than symbiotic mycorrhizas to enhance the phytoextraction by hyperaccumulators. Copyright © 2012 Elsevier B.V. All rights reserved.

The effect of EDTA in attachment gain and root coverage.

PubMed

Kassab, Moawia M; Cohen, Robert E; Andreana, Sebastiano; Dentino, Andrew R

2006-06-01

Root surface biomodification using low pH agents such as citric acid and tetracycline has been proposed to enhance root coverage following connective tissue grafting. The authors hypothesized that root conditioning with neutral pH edetic acid would improve vertical recession depth, root surface coverage, pocket depth, and clinical attachment levels. Twenty teeth in 10 patients with Miller class I and II recession were treated with connective tissue grafting. The experimental sites received 24% edetic acid in sterile distilled water applied to the root surface for 2 minutes before grafting. Controls were pretreated with only sterile distilled water. Measurements were evaluated before surgery and 6 months after surgery. Analysis of variance was used to determine differences between experimental and control groups. We found significant postoperative improvements in vertical recession depth, root surface coverage, and clinical attachment levels in test and control groups, compared to postoperative data. Pocket depth differences were not significant (P<.01).

Intervention strategies to eliminate truck-related fatalities in surface coal mining in West Virginia.

PubMed

Zhang, Meng; Kecojevic, Vladislav

2016-01-01

The main objective of this review was to build upon a previous study on the root causes of truck-related fatalities in surface coal mining operations in West Virginia, and to develop intervention strategies to eliminate these fatalities. This review considers a two-pronged approach to accident prevention: one that is fundamental and traditional (safety regulations, training and education, and engineering of the work environment); and one that is innovative and creative (e.g., applying technological advances to better control and eliminate the root causes of accidents). Suggestions for improving current training and education system are proposed, and recommendations are provided on improving the safety of mine working conditions, specifically safety conditions on haul roads, dump sites, and loading areas. We also discuss various currently available technologies that can help prevent haul truck-related fatal accidents. The results of this review should be used by mine personnel to help create safer working conditions and decrease truck-related fatalities in surface coal mining.

Soil compaction related to grazing and its effects on herbaceous roots frequency and soil organic matter content in rangelands of SW Spain

NASA Astrophysics Data System (ADS)

Pulido, Manuel; Schnabel, Susanne; Francisco Lavado Contador, Joaquín; Miralles Mellado, Isabel

2016-04-01

Rangelands in SW Spain occupy a total surface area of approximately 6 million ha and constitute the most representative extensive ranching system of the Iberian Peninsula gathering more than 13 million livestock heads. They are characterised by an herbaceous layer, mostly composed of therophytic species, with a disperse tree cover, mainly holm oak and cork oak (Quercus ilex rotundifolia and Q. suber), interspersed with shrubs in many places. This type of land system is of ancient origin and experienced frequent changes in land use in the past, since agricultural, livestock and forestry activities have coexisted within the same farms. In recent decades, livestock farming has become dominant due, in part, to the subsidies of the Common Agriculture Policy. Since Spain joined the European Union in 1986 until the year 2000, the number of domestic animals doubled, particularly cattle, and consequently animal stocking rates have increased on average from 0.40 AU ha-1 up to 0.70 AU ha-1. This increase in animal stocking rates, along with a progressive substitution of cattle instead of sheep in many farms, has led to the occurrence of land degradation processes such as the reduction of grass cover or soil compaction in heavily grazed areas. Previous research has evidenced higher values of soil bulk density and resistance to penetration as well as larger bare surface areas in spring in fenced areas with animal stocking rates above 1 AU ha-1. However, a better understanding of how increasing bulk density or resistance to penetration influence the frequency of herbaceous roots and how a reduction in the frequency of roots affects soil organic matter content in rangelands is still unknown. Therefore, the main goal of this study was to determine possible relationships between the frequencies of herbaceous roots and soil organic matter content in order to understand the effect of excessive animal numbers on the depletion of soil fertility by reducing progressively the quantity of roots. The study was carried out in 22 fenced areas belonging to 10 privately-owned farms grazed under different intensities (range: 0.19 - 15.76 AU ha-1 year-1). A total number of 597 undisturbed core samples and 311 bulk samples were collected in order to determine bulk density and soil organic matter content, among other many properties. Resistance to soil penetration was quantified using Eijkelkamp Penetrologger in 890 random points at different depth (0-80 cm) and soil moisture content (dry and wet). The frequency of herbaceous roots was estimated for each soil horizon in 47 soil profiles, described according to FAO and categorized in 4 classes: none-few, common, abundant and many. All variables were pair-wise correlated and their mean values were compared between the different classes of root frequency. Results showed an inverse relationship between soil resistance to penetration and the frequency of herbaceous roots, i.e. the amount of roots lower in soils with high resistance to penetration. Soil horizons with none or few, common, abundant and many roots averaged 0.74%, 1.27%, 2.23% and 2.70% of SOM, respectively, showing significant differences between groups. Furthermore, SOM showed negative coefficients of correlation with resistance to penetration under dry (r=-0.34, p<0.05) and under wet conditions (r=-0.11, p<0.05) and bulk density (r=-0.31, p<0.05). The values herein observed confirm that soil compaction provoked by an excessive number of animals can decrease the content of soil organic matter by reducing the quantity of herbaceous roots although further research is still needed.

Vadose zone microbiology

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kieft, Thomas L.; Brockman, Fred J.

2001-01-17

The vadose zone is defined as the portion of the terrestrial subsurface that extends from the land surface downward to the water table. As such, it comprises the surface soil (the rooting zone), the underlying subsoil, and the capillary fringe that directly overlies the water table. The unsaturated zone between the rooting zone and the capillary fringe is termed the "intermediate zone" (Chapelle, 1993). The vadose zone has also been defined as the unsaturated zone, since the sediment pores and/or rock fractures are generally not completely water filled, but instead contain both water and air. The latter characteristic results inmore » the term "zone of aeration" to describe the vadose zone. The terms "vadose zone," "unsaturated zone", and "zone of aeration" are nearly synonymous, except that the vadose zone may contain regions of perched water that are actually saturated. The term "subsoil" has also been used for studies of shallow areas of the subsurface immediately below the rooting zone. This review focuses almost exclusively on the unsaturated region beneath the soil layer since there is already an extensive body of literature on surface soil microbial communities and process, e.g., Paul and Clark (1989), Metting (1993), Richter and Markowitz, (1995), and Sylvia et al. (1998); whereas the deeper strata of the unsaturated zone have only recently come under scrutiny for their microbiological properties.« less

Phenotypic variation of Pseudomonas brassicacearum as a plant root-colonization strategy.

PubMed

Achouak, Wafa; Conrod, Sandrine; Cohen, Valérie; Heulin, Thierry

2004-08-01

Pseudomonas brassicacearum was isolated as a major root-colonizing population from Arabidopsis thaliana. The strain NFM421 of P. brassicacearum undergoes phenotypic variation during A. thaliana and Brassica napus root colonization in vitro as well as in soil, resulting in different colony appearance on agar surfaces. Bacteria forming translucent colonies (phase II cells) essentially were localized at the surface of young roots and root tips, whereas wild-type cells (phase I cells) were localized at the basal part of roots. The ability of phase II cells to spread and colonize new sites on root surface correlates with over-production of flagellin as evidenced by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of surface proteins and microsequencing. Moreover, phase II cells showed a higher ability to swim and to swarm on semisolid agar medium. Phase I and phase II cells of P. brassicacearum NFM421 were tagged genetically with green fluorescent protein and red fluorescent protein. Confocal scanning laser microscopy was used to localize phase II cells on secondary roots and root tips of A. thaliana, whereas phase I cells essentially were localized at the basal part of roots. These experiments were conducted in vitro and in soil. Phenotypic variation on plant roots is likely to be a colonization strategy that may explain the high colonization power of P. brassicacearum.

The impact of surface area, volume, curvature, and Lennard-Jones potential to solvation modeling.

PubMed

Nguyen, Duc D; Wei, Guo-Wei

2017-01-05

This article explores the impact of surface area, volume, curvature, and Lennard-Jones (LJ) potential on solvation free energy predictions. Rigidity surfaces are utilized to generate robust analytical expressions for maximum, minimum, mean, and Gaussian curvatures of solvent-solute interfaces, and define a generalized Poisson-Boltzmann (GPB) equation with a smooth dielectric profile. Extensive correlation analysis is performed to examine the linear dependence of surface area, surface enclosed volume, maximum curvature, minimum curvature, mean curvature, and Gaussian curvature for solvation modeling. It is found that surface area and surfaces enclosed volumes are highly correlated to each other's, and poorly correlated to various curvatures for six test sets of molecules. Different curvatures are weakly correlated to each other for six test sets of molecules, but are strongly correlated to each other within each test set of molecules. Based on correlation analysis, we construct twenty six nontrivial nonpolar solvation models. Our numerical results reveal that the LJ potential plays a vital role in nonpolar solvation modeling, especially for molecules involving strong van der Waals interactions. It is found that curvatures are at least as important as surface area or surface enclosed volume in nonpolar solvation modeling. In conjugation with the GPB model, various curvature-based nonpolar solvation models are shown to offer some of the best solvation free energy predictions for a wide range of test sets. For example, root mean square errors from a model constituting surface area, volume, mean curvature, and LJ potential are less than 0.42 kcal/mol for all test sets. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Hydraulic conductance as well as nitrogen accumulation plays a role in the higher rate of leaf photosynthesis of the most productive variety of rice in Japan

PubMed Central

Taylaran, Renante D.; Adachi, Shunsuke; Ookawa, Taiichiro; Usuda, Hideaki; Hirasawa, Tadashi

2011-01-01

An indica variety Takanari is known as one of the most productive rice varieties in Japan and consistently produces 20–30% heavier dry matter during ripening than Japanese commercial varieties in the field. The higher rate of photosynthesis of individual leaves during ripening has been recognized in Takanari. By using pot-grown plants under conditions of minimal mutual shading, it was confirmed that the higher rate of leaf photosynthesis is responsible for the higher dry matter production after heading in Takanari as compared with a japonica variety, Koshihikari. The rate of leaf photosynthesis and shoot dry weight became larger in Takanari after the panicle formation and heading stages, respectively, than in Koshihikari. Roots grew rapidly in the panicle formation stage until heading in Takanari compared with Koshihikari. The higher rate of leaf photosynthesis in Takanari resulted not only from the higher content of leaf nitrogen, which was caused by its elevated capacity for nitrogen accumulation, but also from higher stomatal conductance. When measured under light-saturated conditions, stomatal conductance was already decreased due to the reduction in leaf water potential in Koshihikari even under conditions of a relatively small difference in leaf–air vapour pressure difference. In contrast, the higher stomatal conductance was supported by the maintenance of higher leaf water potential through the higher hydraulic conductance in Takanari with the larger area of root surface. However, no increase in root hydraulic conductivity was expected in Takanari. The larger root surface area of Takanari might be a target trait in future rice breeding for increasing dry matter production. PMID:21527630

Hydraulic conductance as well as nitrogen accumulation plays a role in the higher rate of leaf photosynthesis of the most productive variety of rice in Japan.

PubMed

Taylaran, Renante D; Adachi, Shunsuke; Ookawa, Taiichiro; Usuda, Hideaki; Hirasawa, Tadashi

2011-07-01

An indica variety Takanari is known as one of the most productive rice varieties in Japan and consistently produces 20-30% heavier dry matter during ripening than Japanese commercial varieties in the field. The higher rate of photosynthesis of individual leaves during ripening has been recognized in Takanari. By using pot-grown plants under conditions of minimal mutual shading, it was confirmed that the higher rate of leaf photosynthesis is responsible for the higher dry matter production after heading in Takanari as compared with a japonica variety, Koshihikari. The rate of leaf photosynthesis and shoot dry weight became larger in Takanari after the panicle formation and heading stages, respectively, than in Koshihikari. Roots grew rapidly in the panicle formation stage until heading in Takanari compared with Koshihikari. The higher rate of leaf photosynthesis in Takanari resulted not only from the higher content of leaf nitrogen, which was caused by its elevated capacity for nitrogen accumulation, but also from higher stomatal conductance. When measured under light-saturated conditions, stomatal conductance was already decreased due to the reduction in leaf water potential in Koshihikari even under conditions of a relatively small difference in leaf-air vapour pressure difference. In contrast, the higher stomatal conductance was supported by the maintenance of higher leaf water potential through the higher hydraulic conductance in Takanari with the larger area of root surface. However, no increase in root hydraulic conductivity was expected in Takanari. The larger root surface area of Takanari might be a target trait in future rice breeding for increasing dry matter production.

A proposed role for selective autophagy in regulating auxin-dependent lateral root development under phosphate starvation in Arabidopsis.

PubMed

Sankaranarayanan, Subramanian; Samuel, Marcus A

2015-01-01

Plants respond to limited soil nutrient availability by inducing more lateral roots (LR) to increase the root surface area. At the cellular level, nutrient starvation triggers the process of autophagy through which bulk degradation of cellular materials is achieved to facilitate nutrient mobilization. Whether there is any link between the cellular autophagy and induction of LR had remained unknown. We recently showed that the S-Domain receptor Kinase (ARK2) and U Box/Armadillo Repeat-Containing E3 ligase (PUB9) module is required for lateral root formation under phosphate starvation in Arabidopsis thaliana.(1) We also showed that PUB9 localized to autophagic bodies following either activation by ARK2 or under phosphate starvation and ark2-1/pub9-1 plants displayed lateral root defects with inability to accumulate auxin in the root tips under phosphate starvation.(1) Supplementing exogenous auxin was sufficient to rescue the LR defects in ark2-1/pub9-1 mutant. Blocking of autophagic responses in wild-type Arabidopsis also resulted in inhibition of both lateral roots and auxin accumulation in the root tips indicating the importance of autophagy in mediating auxin accumulation under phosphate starved conditions.(1) Here, we propose a model for ARK2/AtPUB9 module in regulation of lateral root development via selective autophagy.

Characterizing roots and water uptake in a ground cover rice production system

PubMed Central

Li, Sen; Zuo, Qiang; Wang, Xiaoyu; Ma, Wenwen; Jin, Xinxin; Shi, Jianchu; Ben-Gal, Alon

2017-01-01

Background and aims Water-saving ground cover rice production systems (GCRPS) are gaining popularity in many parts of the world. We aimed to describe the characteristics of root growth, morphology, distribution, and water uptake for a GCRPS. Methods A traditional paddy rice production system (TPRPS) was compared with GCRPS in greenhouse and field experiments. In the greenhouse, GCRPS where root zone average soil water content was kept near saturation (GCRPSsat), field capacity (GCRPSfwc) and 80% field capacity (GCRPS80%), were evaluated. In a two-year field experiment, GCRPSsat and GCRPS80% were applied. Results Similar results were found in greenhouse and field experiments. Before mid-tillering the upper soil temperature was higher for GCRPS, leading to enhanced root dry weight, length, surface area, specific root length, and smaller diameter of roots but lower water uptake rate per root length compared to TPRPS. In subsequent growth stages, the reduced soil water content under GCRPS caused that the preponderance of root growth under GCRPSsat disappeared in comparison to TPRPS. Under other GCRPS treatments (GCRPSfwc and GCRPS80%), significant limitation on root growth, bigger root diameter and higher water uptake rate per root length were found. Conclusions Discrepancies in soil water and temperature between TPRPS and GCRPS caused adjustments to root growth, morphology, distribution and function. Even though drought stress was inevitable after mid-tillering under GCRPS, especially GCRPS80%, similar or even enhanced root water uptake capacity in comparison to TPRPS might promote allocation of photosynthetic products to shoots and increase water productivity. PMID:28686687

Characterizing pathways by which gravitropic effectors could move from the root cap to the root of primary roots of Zea mays

NASA Technical Reports Server (NTRS)

Moore, R.; McClelen, C. E.

1989-01-01

Plasmodesmata linking the root cap and root in primary roots Zea mays are restricted to approx. 400 protodermal cells bordering approx. 110000 microns2 of the calyptrogen of the root cap. This area is less than 10% of the cross-sectional area of the root-tip at the cap junction. Therefore, gravitropic effectors moving from the root cap to the root can move symplastically only through a relatively small area in the centre of the root. Decapped roots are non-responsive to gravity. However, decapped roots whose caps are replaced immediately after decapping are strongly graviresponsive. Thus, gravicurvature occurs only when the root cap contacts the root, and symplastic continuity between the cap and root is not required for gravicurvature. Completely removing mucilage from the root tip renders the root non-responsive to gravity. Taken together, these data suggest that gravitropic effectors move apoplastically through mucilage from the cap to the root.

Comparison of Tibiofemoral Contact Mechanics After Various Transtibial and All-Inside Fixation Techniques for Medial Meniscus Posterior Root Radial Tears in a Porcine Model.

PubMed

Chung, Kyu Sung; Choi, Choong Hyeok; Bae, Tae Soo; Ha, Jeong Ku; Jun, Dal Jae; Wang, Joon Ho; Kim, Jin Goo

2018-04-01

To compare tibiofemoral contact mechanics after fixation for medial meniscus posterior root radial tears (MMPRTs). Seven fresh knees from mature pigs were used. Each knee was tested under 5 conditions: normal knee, MMPRT, pullout fixation with simple sutures, fixation with modified Mason-Allen sutures, and all-inside fixation using Fastfix 360. The peak contact pressure and contact surface area were evaluated using a capacitive sensor positioned between the meniscus and tibial plateau, under a 1,000-N compression force, at different flexion angles (0°, 30°, 60°, and 90°). The peak contact pressure was significantly higher in MMPRTs than in normal knees (P = .018). Although the peak contact pressure decreased significantly after fixation at all flexion angles (P = .031), it never recovered to the values noted in the normal meniscus. No difference was observed among fixation groups (P = .054). The contact surface area was significantly lower in MMPRTs than in the normal meniscus (P = .018) and increased significantly after fixation at all flexion angles (P = .018) but did not recover to within normal limits. For all flexion angles except 60°, the contact surface area was significantly higher for fixation with Mason-Allen sutures than for fixation with simple sutures or all-inside fixation (P = .027). At 90° of flexion, the contact surface area was significantly better for fixation with simple sutures than for all-inside fixation (P = .031). The peak contact pressure and contact surface area improved significantly after fixation, regardless of the fixation method, but did not recover to the levels noted in the normal meniscus after any type of fixation. Among the fixation methods evaluated in this time 0 study, fixation using modified Mason-Allen sutures provided a superior contact surface area compared with that noted after fixation using simple sutures or all-inside fixation, except at 60° of flexion. However, this study had insufficient power to accurately detect the differences between the outcomes of various fixation methods. Our results in a porcine model suggest that fixation can restore tibiofemoral contact mechanics in MMPRT and that fixation with a locking mechanism leads to superior biomechanical properties. Copyright © 2017 Arthroscopy Association of North America. Published by Elsevier Inc. All rights reserved.

The Function of the Superficial Root Mat in the Biogeochemical Cycles of Nutrients in Congolese Eucalyptus Plantations

PubMed Central

LACLAU, JEAN‐PAUL; TOUTAIN, FRANÇOIS; M’BOU, ARMEL THONGO; ARNAUD, MICHEL; JOFFRE, RICHARD; RANGER, JACQUES

2004-01-01

• Background and Aims The importance of superficial root mats inside the forest floor for the nutrition of Amazonian rain forests has been extensively investigated. The present study was aimed at assessing the function of a root mat adherent to decomposing organic material observed in Eucalyptus plantations. • Methods The development of the root mat was studied through micromorphological observations of thin litter sections, and the influence of soil microtopography and soil water repellency on root mat biomass was assessed in situ on an area of 5 m2. In addition, input–output budgets of nutrients within the forest floor were established from measurements of litterfall, dissolved nutrients in gravitational solutions, and forest floor nutrient contents. • Key Findings The amounts of nutrients released during litter decay in this ecosystem during the period of study were, on average, 46, 3, 4, 19 and 17 kg ha–1 year–1 for N, P, K, Ca and Mg, respectively. The simultaneous measurements of the chemical composition of throughfall solutions and leachates beneath the forest floor showed a very quick uptake of nutrients by the root mat during the decomposition processes. Indeed, the solutions did not become noticeably enriched in nutrients during their passage through the holorganic layer, despite large amounts of elements being released during litter decay. The root mat biomass decreased significantly during the dry season, and a preferential development in microdepressions at the soil surface was observed. A strong water repellency observed in these depressions might enhance the ability of the roots to take up water and nutrients during the dry periods. • Conclusions The root mat was active throughout the year to catch the flux of nutrients from the biodegradation of the forest floor, preventing the transfer of dissolved nutrients toward deeper soil horizons. This mechanism is involved in the successful adaptation of this Eucalyptus hybrid in areas covered by ‘climacic’ savannas in Congo. PMID:14749252

Three-rooted premolar analyzed by high-resolution and cone beam CT.

PubMed

Marca, Caroline; Dummer, Paul M H; Bryant, Susan; Vier-Pelisser, Fabiana Vieira; Só, Marcus Vinicius Reis; Fontanella, Vania; Dutra, Vinicius D'avila; de Figueiredo, José Antonio Poli

2013-07-01

The aim of this study was to analyze the variations in canal and root cross-sectional area in three-rooted maxillary premolars between high-resolution computed tomography (μCT) and cone beam computed tomography (CBCT). Sixteen extracted maxillary premolars with three distinct roots and fully formed apices were scanned using μCT and CBCT. Photoshop CS software was used to measure root and canal cross-sectional areas at the most cervical and the most apical points of each root third in images obtained using the two tomographic computed (CT) techniques, and at 30 root sections equidistant from both root ends using μCT images. Canal and root areas were compared between each method using the Student t test for paired samples and 95 % confidence intervals. Images using μCT were sharper than those obtained using CBCT. There were statistically significant differences in mean area measurements of roots and canals between the μCT and CBCT techniques (P < 0.05). Root and canal areas had similar variations in cross-sectional μCT images and became proportionally smaller in a cervical to apical direction as the cementodentinal junction was approached, from where the area then increased apically. Although variation was similar in the roots and canals under study, CBCT produced poorer image details than μCT. Although CBCT is a strong diagnosis tool, it still needs improvement to provide accuracy in details of the root canal system, especially in cases with anatomical variations, such as the three-rooted maxillary premolars.

[Water-saving mechanisms of intercropping system in improving cropland water use efficiency].

PubMed

Zhang, Feng-Yun; Wu, Pu-Te; Zhao, Xi-Ning; Cheng, Xue-Feng

2012-05-01

Based on the multi-disciplinary researches, and in terms of the transformation efficiency of surface water to soil water, availability of cropland soil water, crop canopy structure, total irrigation volume needed on a given area, and crop yield, this paper discussed the water-saving mechanisms of intercropping system in improving cropland water use efficiency. Intercropping system could promote the full use of cropland water by plant roots, increase the water storage in root zone, reduce the inter-row evaporation and control excessive transpiration, and create a special microclimate advantageous to the plant growth and development. In addition, intercropping system could optimize source-sink relationship, provide a sound foundation for intensively utilizing resources temporally and spatially, and increase the crop yield per unit area greatly without increase of water consumption, so as to promote the crop water use efficiency effectively.

Analysis of turbulence and surface growth models on the estimation of soot level in ethylene non-premixed flames

NASA Astrophysics Data System (ADS)

Yunardi, Y.; Munawar, Edi; Rinaldi, Wahyu; Razali, Asbar; Iskandar, Elwina; Fairweather, M.

2018-02-01

Soot prediction in a combustion system has become a subject of attention, as many factors influence its accuracy. An accurate temperature prediction will likely yield better soot predictions, since the inception, growth and destruction of the soot are affected by the temperature. This paper reported the study on the influences of turbulence closure and surface growth models on the prediction of soot levels in turbulent flames. The results demonstrated that a substantial distinction was observed in terms of temperature predictions derived using the k-ɛ and the Reynolds stress models, for the two ethylene flames studied here amongst the four types of surface growth rate model investigated, the assumption of the soot surface growth rate proportional to the particle number density, but independent on the surface area of soot particles, f ( A s ) = ρ N s , yields in closest agreement with the radial data. Without any adjustment to the constants in the surface growth term, other approaches where the surface growth directly proportional to the surface area and square root of surface area, f ( A s ) = A s and f ( A s ) = √ A s , result in an under- prediction of soot volume fraction. These results suggest that predictions of soot volume fraction are sensitive to the modelling of surface growth.

Effects of Inundation, Nutrient Availability and Plant Species Diversity on Fine Root Mass and Morphology Across a Saltmarsh Flooding Gradient

PubMed Central

Redelstein, Regine; Dinter, Thomas; Hertel, Dietrich; Leuschner, Christoph

2018-01-01

Saltmarsh plants are exposed to multiple stresses including tidal inundation, salinity, wave action and sediment anoxia, which require specific root system adaptations to secure sufficient resource capture and firm anchorage in a temporary toxic environment. It is well known that many saltmarsh species develop large below-ground biomass (roots and rhizomes) but relations between fine roots, in particular, and the abiotic conditions in salt marshes are widely unknown. We studied fine root mass (<2 mm in diameter), fine root depth distribution and fine root morphology in three typical communities (Spartina anglica-dominated pioneer zone, Atriplex portulacoides-dominated lower marsh, Elytrigia atherica-dominated upper marsh) across elevational gradients in two tidal salt marshes of the German North Sea coast [a mostly sandy marsh on a barrier island (Spiekeroog), and a silty-clayey marsh on the mainland coast (Westerhever)]. Fine root mass in the 0–40 cm profile ranged between 750 and 2,500 g m−2 in all plots with maxima at both sites in the lower marsh with intermediate inundation frequency and highest plant species richness indicating an effect of biodiversity on fine root mass. Fine root mass and, even more, total fine root surface area (maximum 340 m2 m−2) were high compared to terrestrial grasslands, and were greater in the nutrient-poorer Spiekeroog marsh. Fine root density showed only a slight or no decrease toward 40 cm depth. We conclude that the standing fine root mass and morphology of these salt marshes is mainly under control of species identity and nutrient availability, but species richness is especially influential. The plants of the pioneer zone and lower marsh possess well adapted fine roots and large standing root masses despite the often water-saturated sediment. PMID:29467778

Deposition of corrosion products from dowels on human dental root surfaces measured with proton microprobe technique

NASA Astrophysics Data System (ADS)

Brune, D.; Brunell, G.; Lindh, U.

1982-06-01

Distribution of copper, mercury and zinc on human teeth root surfaces adjacent to dowels of gold alloy or brass as well as dowels of brass in conjunction with an amalgam crown has been measured with a proton microprobe using PIXE techniques. Upper limits of the contents of gold and silver on the root surfaces were established. Pronounced concentration profiles of copper and zinc were observed on the root surfaces of teeth prepared with dowels of brass. The dowel of gold alloy revealed only zinc deposition. The major part of copper on the root surfaces is assumed to arise from corrosion of the dowels, and has been transported to the surface by diffusion through the dential tubuli. Zinc in the volume analysed is a constituent of dentin tissue as well as a corrosion product of the brass dowel. Part of the zinc level could also be ascribed to erosion of the zinc phosphate cement matrix. The volumes analysed were (25×25×25)μm 3. The levels of copper, mercury and zinc on the tooth root surfaces attained values up to about 200, 20 and 600 ppm, respectively.

[Root system distribution and biomechanical characteristics of Bambusa oldhami].

PubMed

Zhou, Ben-Zhi; Xu, Sheng-Hua; An, Yan-Fei; Xu, Sheng-Hua

2014-05-01

To determine the mechanism of soil stabilizing through Bambusa oldhami root system, the vertical distribution of B. oldhami root system in soil was investigated, and the tensile strength of individual root and soil shear strength were measured in B. oldhami forest. The dry mass, length, surface area and volume of the B. oldhami root system decreased with the increasing soil depth, with more than 90% of the root system occurring in the 0-40 cm soil layer. The root class with D 1 mm occupied the highest percentage of the total in terms of root length, accounting for 79.6%, but the lowest percentage of the total in terms of root volume, accounting for 8.2%. The root class with D >2 mm was the opposite, and the root class with D= 1-2 mm stayed in between. The maximum tensile resistance of B. oldhami root, either with 12% moisture content or a saturated moisture content, increased with the increasing root diameter, while the tensile strength decreased with the increasing root diameter in accordance with power function. Tensile strength of the root, with either of the two moisture contents, was significantly different among the diameter classes, with the highest tensile strength occurring in the root with D < or = 1 mm and the lowest in the root with D > or = 2 mm. The tensile strength of root with 12% moisture content was significantly higher than that with the saturated moisture content, and less effect of moisture content on root tensile strength would occur in thicker roots. The shear strengths of B. oldhami forest soil and of bare soil both increased with the increasing soil depth. The shear strength of B. oldhami forest soil had a linear positive correlation with the root content in soil, and was significantly higher than that of bare soil. The shear strength increment in B. oldhami forest was positively correlated with the root content in soil according to an exponential function, but not related significantly with soil depth.

Effect of Liquid Surface Turbulent Motion on the Vapor Condensation in a Mixing Tank

NASA Technical Reports Server (NTRS)

Lin, C. S.; Hasan, M. M.

1991-01-01

The effect of liquid surface motion on the vapor condensation in a tank mixed by an axial turbulent jet is numerically investigated. The average value (over the interface area) of the root-mean-squared (rms) turbulent velocity at the interface is shown to be linearly increasing with decreasing liquid height and increasing jet diameter for a given tank size. The average rms turbulent velocity is incorporated in Brown et al. (1990) condensation correlation to predict the condensation of vapor on a liquid surface. The results are in good agreement with available condensation data.

[The use of nucleolar morphological characteristics of birch seedlings for the assessment of environmental pollution].

PubMed

Karpova, S S; Kalaev, V N; Artiukov, V G; Trofimova, V A; OstashkovaL G, A D; Savko

2006-01-01

Micronucleus frequency in buccal mucosa from the oral cavity of children as well as nucleolar structural characteristics (surface area of single nucleoli as well as their number and type) in the root meristem of seed progeny of birch (Betula pendula Roth) were studied in some districts of Voronezh City and Voronezh Region (Novovoronezh Town, Zemlyansk Village). Similar trends of changes in cytogenetic parameters have been revealed for both subjects. Regression analysis allowed us to generate an equation relating the cytogenetic parameters of birch seed progeny (surface area of single nucleoli) and humans (frequency of micronuclei in buccal mucosa of children). This study can be considered as a result of cytogenetic monitoring of environmental pollution in some areas of Voronezh City and Voronezh Region.

Surface-based GPR underestimates below-stump root biomass

Treesearch

John R. Butnor; Lisa J. Samuelson; Thomas A. Stokes; Kurt H. Johnsen; Peter H. Anderson; Carlos A. Gonzalez-Benecke

2016-01-01

Aims While lateral root mass is readily detectable with ground penetrating radar (GPR), the roots beneath a tree (below-stump) and overlapping lateral roots near large trees are problematic for surface-based antennas operated in reflection mode. We sought to determine if tree size (DBH) effects GPR root detection proximal to longleaf pine (Pinus palustris Mill) and if...

Can we manipulate root system architecture to control soil erosion?

NASA Astrophysics Data System (ADS)

Ola, A.; Dodd, I. C.; Quinton, J. N.

2015-09-01

Soil erosion is a major threat to soil functioning. The use of vegetation to control erosion has long been a topic for research. Much of this research has focused on the above-ground properties of plants, demonstrating the important role that canopy structure and cover plays in the reduction of water erosion processes. Less attention has been paid to plant roots. Plant roots are a crucial yet under-researched factor for reducing water erosion through their ability to alter soil properties, such as aggregate stability, hydraulic function and shear strength. However, there have been few attempts to specifically manipulate plant root system properties to reduce soil erosion. Therefore, this review aims to explore the effects that plant roots have on soil erosion and hydrological processes, and how plant root architecture might be manipulated to enhance its erosion control properties. We demonstrate the importance of root system architecture for the control of soil erosion. We also show that some plant species respond to nutrient-enriched patches by increasing lateral root proliferation. The erosional response to root proliferation will depend upon its location: at the soil surface dense mats of roots may reduce soil erodibility but block soil pores thereby limiting infiltration, enhancing runoff. Additionally, in nutrient-deprived regions, root hair development may be stimulated and larger amounts of root exudates released, thereby improving aggregate stability and decreasing erodibility. Utilizing nutrient placement at specific depths may represent a potentially new, easily implemented, management strategy on nutrient-poor agricultural land or constructed slopes to control erosion, and further research in this area is needed.

Phenotyping: Using Machine Learning for Improved Pairwise Genotype Classification Based on Root Traits

PubMed Central

Zhao, Jiangsan; Bodner, Gernot; Rewald, Boris

2016-01-01

Phenotyping local crop cultivars is becoming more and more important, as they are an important genetic source for breeding – especially in regard to inherent root system architectures. Machine learning algorithms are promising tools to assist in the analysis of complex data sets; novel approaches are need to apply them on root phenotyping data of mature plants. A greenhouse experiment was conducted in large, sand-filled columns to differentiate 16 European Pisum sativum cultivars based on 36 manually derived root traits. Through combining random forest and support vector machine models, machine learning algorithms were successfully used for unbiased identification of most distinguishing root traits and subsequent pairwise cultivar differentiation. Up to 86% of pea cultivar pairs could be distinguished based on top five important root traits (Timp5) – Timp5 differed widely between cultivar pairs. Selecting top important root traits (Timp) provided a significant improved classification compared to using all available traits or randomly selected trait sets. The most frequent Timp of mature pea cultivars was total surface area of lateral roots originating from tap root segments at 0–5 cm depth. The high classification rate implies that culturing did not lead to a major loss of variability in root system architecture in the studied pea cultivars. Our results illustrate the potential of machine learning approaches for unbiased (root) trait selection and cultivar classification based on rather small, complex phenotypic data sets derived from pot experiments. Powerful statistical approaches are essential to make use of the increasing amount of (root) phenotyping information, integrating the complex trait sets describing crop cultivars. PMID:27999587

SMERGE: A multi-decadal root-zone soil moisture product for CONUS

NASA Astrophysics Data System (ADS)

Crow, W. T.; Dong, J.; Tobin, K. J.; Torres, R.

2017-12-01

Multi-decadal root-zone soil moisture products are of value for a range of water resource and climate applications. The NASA-funded root-zone soil moisture merging project (SMERGE) seeks to develop such products through the optimal merging of land surface model predictions with surface soil moisture retrievals acquired from multi-sensor remote sensing products. This presentation will describe the creation and validation of a daily, multi-decadal (1979-2015), vertically-integrated (both surface to 40 cm and surface to 100 cm), 0.125-degree root-zone product over the contiguous United States (CONUS). The modeling backbone of the system is based on hourly root-zone soil moisture simulations generated by the Noah model (v3.2) operating within the North American Land Data Assimilation System (NLDAS-2). Remotely-sensed surface soil moisture retrievals are taken from the multi-sensor European Space Agency Climate Change Initiative soil moisture data set (ESA CCI SM). In particular, the talk will detail: 1) the exponential smoothing approach used to convert surface ESA CCI SM retrievals into root-zone soil moisture estimates, 2) the averaging technique applied to merge (temporally-sporadic) remotely-sensed with (continuous) NLDAS-2 land surface model estimates of root-zone soil moisture into the unified SMERGE product, and 3) the validation of the SMERGE product using long-term, ground-based soil moisture datasets available within CONUS.

Superior Root Hair Formation Confers Root Efficiency in Some, But Not All, Rice Genotypes upon P Deficiency.

PubMed

Nestler, Josefine; Wissuwa, Matthias

2016-01-01

Root hairs are a low-cost way to extend root surface area (RSA), water and nutrient acquisition. This study investigated to what extend variation exists for root hair formation in rice in dependence of genotype, phosphorus (P) supply, growth medium, and root type. In general, genotypic variation was found for three root hair properties: root hair length, density, and longevity. In low P nutrient solution more than twofold genotypic difference was detected for root hair length while only onefold variation was found in low P soil. These differences were mostly due to the ability of some genotypes to increase root hair length in response to P deficiency. In addition, we were able to show that a higher proportion of root hairs remain viable even in mature, field-grown plants under low P conditions. All investigated root hair parameters exhibited high correlations across root types which were always higher in the low P conditions compared to the high P controls. Therefore we hypothesize that a low P response leads to a systemic signal in the entire root system. The genotype DJ123 consistently had the longest root hairs under low P conditions and we estimated that, across the field-grown root system, root hairs increased the total RSA by 31% in this genotype. This would explain why DJ123 is considered to be very root efficient in P uptake and suggests that DJ123 should be utilized as a donor in breeding for enhanced P uptake. Surprisingly, another root and P efficient genotype seemed not to rely on root hair growth upon P deficiency and therefore must contain different methods of low P adaptation. Genotypic ranking of root hair properties did change substantially with growth condition highlighting the need to phenotype plants in soil-based conditions or at least to validate results obtained in solution-based growth conditions.

First Assessment of Carbon Stock in the Belowground Biomass of Brazilian Mangroves.

PubMed

Santos, Daniel M C; Estrada, Gustavo C D; Fernandez, Viviane; Estevam, Marciel R M; Souza, Brunna T DE; Soares, Mário L G

2017-01-01

Studies on belowground roots biomass have increasingly reported the importance of the contribution of this compartment in carbon stock maintenance in mangrove forests. To date, there are no estimates of this contribution in Brazilian mangrove forests, although the country has the second largest area of mangroves worldwide. For this study, trenches dug in fringing forests in Guaratiba State Biological Reserve (Rio de Janeiro, Brazil) were used to evaluate the contribution of the different classes of roots and the vertical stratification of carbon stock. The total carbon stock average in belowground roots biomass in these forests was 104.41 ± 20.73 tC.ha-1. From that, an average of 84.13 ± 21.34 tC.ha-1 corresponded to the carbon stock only in fine roots, which have diameters smaller than 5 mm and are responsible for over 80% of the total belowground biomass. Most of the belowground carbon stock is concentrated in the first 40 cm below the surface (about 70%). The root:shoot ratio in this study is 1.14. These estimates demonstrate that the belowground roots biomass significantly contributes, more than 50%, to the carbon stock in mangrove forests. And the mangrove root biomass can be greater than that of other Brazilian ecosystems.

Surface modification of tooth root canal after application of an X-ray opaque waveguide

NASA Astrophysics Data System (ADS)

Dostálová, T.; Jelínková, H.; Šulc, J.; Němec, M.; Koranda, P.; Bartoňová, M.; Radina, P.; Miyagi, M.; Shi, Y.-W.; Matsuura, Y.

The interest in endodontic use of dental laser systems has been increasing. With the development of thin and flexible delivery systems for various wavelengths, laser applications in endodontics may become even more desirable. The aim of this study is to check the X-ray opacity of a hollow waveguide and to observe the results after laser root canal treatment. The root canal systems of 10 molars were treated endodontically by laser. For the laser radiation source, an Er:YAG laser system generating a wavelength of 2940 nm and an Alexandrite laser system generating a wavelength of 375 nm were used. The hollow waveguide used was checked under X-ray . A root canal surface treated by laser radiation was analyzed by a scanning electron microscope (SEM). The special hollow glass waveguide used was visible in the root canal system under X-ray imaging. Surface modification of the root canal after laser treatment was not found. After conventional treatment the root canal was enlarged. The surface was covered with a smear layer. After application of both laser systems, the smear layer was removed. The resulting canal surface was found to be clean and smooth. Under SEM observation open dentinal tubules were visible. No cracks were present, nor were surface modifications observed.

Vacuum insulation of the high energy negative ion source for fusion application.

PubMed

Kojima, A; Hanada, M; Hilmi, A; Inoue, T; Watanabe, K; Taniguchi, M; Kashiwagi, M; Umeda, N; Tobari, H; Kobayashi, S; Yamano, Y; Grisham, L R

2012-02-01

Vacuum insulation on a large size negative ion accelerator with multiple extraction apertures and acceleration grids for fusion application was experimentally examined and designed. In the experiment, vacuum insulation characteristics were investigated in the JT-60 negative ion source with >1000 apertures on the grid with the surface area of ∼2 m(2). The sustainable voltages varied with a square root of the gap lengths between the grids, and decreased with number of the apertures and with the surface area of the grids. Based on the obtained results, the JT-60SA (super advanced) negative ion source is designed to produce 22 A, 500 keV D(-) ion beams for 100 s.

Calculus removal on a root cement surface by ultrashort laser pulses

NASA Astrophysics Data System (ADS)

Kraft, Johan F.; Vestentoft, Kasper; Christensen, Bjarke H.; Løvschall, Henrik; Balling, Peter

2008-01-01

Ultrashort-pulse-laser ablation of dental calculus (tartar) and cement is performed on root surfaces. The investigation shows that the threshold fluence for ablation of calculus is a factor of two to three times smaller than that of a healthy root cement surface. This indicates that ultrashort laser pulses may provide an appropriate tool for selective removal of calculus with minimal damage to the underlying root cement. Future application of an in situ profiling technique allows convenient on-line monitoring of the ablation process.

Selective laser melting of titanium alloy enables osseointegration of porous multi-rooted implants in a rabbit model.

PubMed

Peng, Wei; Xu, Liangwei; You, Jia; Fang, Lihua; Zhang, Qing

2016-07-21

Osseointegration refers to the direct connection between living bone and the surface of a load-bearing artificial implant. Porous implants with well-controlled porosity and pore size can enhance osseointegration. However, until recently implants were produced by machining solid core titanium rods. The aim of this study was to develop a multi-rooted dental implant (MRI) with a connected porous surface structure to facilitate osseointegration. MRIs manufactured by selective laser melting (SLM) and commercial implants with resorbable blasting media (RBM)-treated surfaces were inserted into the hind limbs of New Zealand white rabbits. Osseointegration was evaluated periodically over 12 weeks by micro-computerized tomography (CT) scanning, histological analysis, mechanical push-out tests, and torque tests. Bone volume densities were consistently higher in the MRI group than in the RBM group throughout the study period, ultimately resulting in a peak value of 48.41 % for the MRI group. Histological analysis revealed denser surrounding bone growth in the MRIs; after 4 and 8 weeks, bone tissue had grown into the pore structures and root bifurcation areas, respectively. Biomechanics tests indicated binding of the porous MRIs to the neobone tissues, as push-out forces strengthened from 294.7 to 446.5 N and maximum mean torque forces improved from 81.15 to 289.57 N (MRI), versus 34.79 to 87.8 N in the RBM group. MRIs manufactured by SLM possess a connected porous surface structure that improves the osteogenic characteristics of the implant surface.

Quantitative, nondestructive estimates of coarse root biomass in a temperate pine forest using 3-D ground-penetrating radar (GPR)

NASA Astrophysics Data System (ADS)

Molon, Michelle; Boyce, Joseph I.; Arain, M. Altaf

2017-01-01

Coarse root biomass was estimated in a temperate pine forest using high-resolution (1 GHz) 3-D ground-penetrating radar (GPR). GPR survey grids were acquired across a 400 m2 area with varying line spacing (12.5 and 25 cm). Root volume and biomass were estimated directly from the 3-D radar volume by using isometric surfaces calculated with the marching cubes algorithm. Empirical relations between GPR reflection amplitude and root diameter were determined for 14 root segments (0.1-10 cm diameter) reburied in a 6 m2 experimental test plot and surveyed at 5-25 cm line spacing under dry and wet soil conditions. Reburied roots >1.4 cm diameter were detectable as continuous root structures with 5 cm line separation. Reflection amplitudes were strongly controlled by soil moisture and decreased by 40% with a twofold increase in soil moisture. GPR line intervals of 12.5 and 25 cm produced discontinuous mapping of roots, and GPR coarse root biomass estimates (0.92 kgC m-2) were lower than those obtained previously with a site-specific allometric equation due to nondetection of vertical roots and roots <1.5 cm diameter. The results show that coarse root volume and biomass can be estimated directly from interpolated 3-D GPR volumes by using a marching cubes approach, but mapping of roots as continuous structures requires high inline sampling and line density (<5 cm). The results demonstrate that 3-D GPR is viable approach for estimating belowground carbon and for mapping tree root architecture. This methodology can be applied more broadly in other disciplines (e.g., archaeology and civil engineering) for imaging buried structures.

Developmental morphology of cover crop species exhibit contrasting behaviour to changes in soil bulk density, revealed by X-ray computed tomography

PubMed Central

Burr-Hersey, Jasmine E.; Mooney, Sacha J.; Bengough, A. Glyn; Mairhofer, Stefan

2017-01-01

Plant roots growing through soil typically encounter considerable structural heterogeneity, and local variations in soil dry bulk density. The way the in situ architecture of root systems of different species respond to such heterogeneity is poorly understood due to challenges in visualising roots growing in soil. The objective of this study was to visualise and quantify the impact of abrupt changes in soil bulk density on the roots of three cover crop species with contrasting inherent root morphologies, viz. tillage radish (Raphanus sativus), vetch (Vicia sativa) and black oat (Avena strigosa). The species were grown in soil columns containing a two-layer compaction treatment featuring a 1.2 g cm-3 (uncompacted) zone overlaying a 1.4 g cm-3 (compacted) zone. Three-dimensional visualisations of the root architecture were generated via X-ray computed tomography, and an automated root-segmentation imaging algorithm. Three classes of behaviour were manifest as a result of roots encountering the compacted interface, directly related to the species. For radish, there was switch from a single tap-root to multiple perpendicular roots which penetrated the compacted zone, whilst for vetch primary roots were diverted more horizontally with limited lateral growth at less acute angles. Black oat roots penetrated the compacted zone with no apparent deviation. Smaller root volume, surface area and lateral growth were consistently observed in the compacted zone in comparison to the uncompacted zone across all species. The rapid transition in soil bulk density had a large effect on root morphology that differed greatly between species, with major implications for how these cover crops will modify and interact with soil structure. PMID:28753645

Developmental morphology of cover crop species exhibit contrasting behaviour to changes in soil bulk density, revealed by X-ray computed tomography.

PubMed

Burr-Hersey, Jasmine E; Mooney, Sacha J; Bengough, A Glyn; Mairhofer, Stefan; Ritz, Karl

2017-01-01

Plant roots growing through soil typically encounter considerable structural heterogeneity, and local variations in soil dry bulk density. The way the in situ architecture of root systems of different species respond to such heterogeneity is poorly understood due to challenges in visualising roots growing in soil. The objective of this study was to visualise and quantify the impact of abrupt changes in soil bulk density on the roots of three cover crop species with contrasting inherent root morphologies, viz. tillage radish (Raphanus sativus), vetch (Vicia sativa) and black oat (Avena strigosa). The species were grown in soil columns containing a two-layer compaction treatment featuring a 1.2 g cm-3 (uncompacted) zone overlaying a 1.4 g cm-3 (compacted) zone. Three-dimensional visualisations of the root architecture were generated via X-ray computed tomography, and an automated root-segmentation imaging algorithm. Three classes of behaviour were manifest as a result of roots encountering the compacted interface, directly related to the species. For radish, there was switch from a single tap-root to multiple perpendicular roots which penetrated the compacted zone, whilst for vetch primary roots were diverted more horizontally with limited lateral growth at less acute angles. Black oat roots penetrated the compacted zone with no apparent deviation. Smaller root volume, surface area and lateral growth were consistently observed in the compacted zone in comparison to the uncompacted zone across all species. The rapid transition in soil bulk density had a large effect on root morphology that differed greatly between species, with major implications for how these cover crops will modify and interact with soil structure.

Contrasting responses of root morphology and root-exuded organic acids to low phosphorus availability in three important food crops with divergent root traits.

PubMed

Wang, Yan-Liang; Almvik, Marit; Clarke, Nicholas; Eich-Greatorex, Susanne; Øgaard, Anne Falk; Krogstad, Tore; Lambers, Hans; Clarke, Jihong Liu

2015-08-17

Phosphorus (P) is an important element for crop productivity and is widely applied in fertilizers. Most P fertilizers applied to land are sorbed onto soil particles, so research on improving plant uptake of less easily available P is important. In the current study, we investigated the responses in root morphology and root-exuded organic acids (OAs) to low available P (1 μM P) and sufficient P (50 μM P) in barley, canola and micropropagated seedlings of potato-three important food crops with divergent root traits, using a hydroponic plant growth system. We hypothesized that the dicots canola and tuber-producing potato and the monocot barley would respond differently under various P availabilities. WinRHIZO and liquid chromatography triple quadrupole mass spectrometry results suggested that under low P availability, canola developed longer roots and exhibited the fastest root exudation rate for citric acid. Barley showed a reduction in root length and root surface area and an increase in root-exuded malic acid under low-P conditions. Potato exuded relatively small amounts of OAs under low P, while there was a marked increase in root tips. Based on the results, we conclude that different crops show divergent morphological and physiological responses to low P availability, having evolved specific traits of root morphology and root exudation that enhance their P-uptake capacity under low-P conditions. These results could underpin future efforts to improve P uptake of the three crops that are of importance for future sustainable crop production. Published by Oxford University Press on behalf of the Annals of Botany Company.

The effect of MTAD, an endodontic irrigant, on fibroblast attachment to periodontally affected root surfaces: A SEM analysis.

PubMed

Ghandi, Mostafa; Houshmand, Behzad; Nekoofar, Mohammad H; Tabor, Rachel K; Yadeghari, Zahra; Dummer, Paul M H

2013-03-01

Root surface debridement (RSD) is necessary to create an environment suitable for reattachment of the periodontium. Root surface conditioning may aid the formation of a biocompatible surface suitable for cell reattachment. BioPure™ MTAD (mixture of Doxycycline, citric acid and a detergent) is an endodontic irrigant with antibacterial properties and the ability to remove smear layer. It was hypothesized that MTAD may be useful for root surface conditioning. The efficacy of MTAD as a conditioner was measured by examining fibroblast attachment to root surfaces. Thirty-two specimens of human teeth with advanced periodontal disease were used. The surfaces were root planed until smooth. Half of the specimens were treated with 0.9% saline and the other samples with Biopure MTAD. As a negative control group, five further samples were left unscaled with surface calculus. Human gingival fibroblast cells HGF1-PI1 were cultured and poured over the tooth specimens and incubated. After fixation, the samples were sputter-coated with gold and examined with a SEM. The morphology and number of attached, fixed viable cells were examined. The data was analysed using the Mann-Whitney-U statistical test. There was no significant difference between the numbers of attached cells in the experimental group treated with MTAD and the control group treated with saline. Little or no attached cells were seen in the negative control group. RSD created an environment suitable for cell growth and attachment in a laboratory setting. The use of MTAD did not promote the attachment and growth of cells on the surface of human roots following RSD.

Role of Subsurface Physics in the Assimilation of Surface Soil Moisture Observations

NASA Technical Reports Server (NTRS)

Reichle, R. H.

2010-01-01

Root zone soil moisture controls the land-atmosphere exchange of water and energy and exhibits memory that may be useful for climate prediction at monthly scales. Assimilation of satellite-based surface soil moisture observations into a land surface model is an effective way to estimate large-scale root zone soil moisture. The propagation of surface information into deeper soil layers depends on the model-specific representation of subsurface physics that is used in the assimilation system. In a suite of experiments we assimilate synthetic surface soil moisture observations into four different models (Catchment, Mosaic, Noah and CLM) using the Ensemble Kalman Filter. We demonstrate that identical twin experiments significantly overestimate the information that can be obtained from the assimilation of surface soil moisture observations. The second key result indicates that the potential of surface soil moisture assimilation to improve root zone information is higher when the surface to root zone coupling is stronger. Our experiments also suggest that (faced with unknown true subsurface physics) overestimating surface to root zone coupling in the assimilation system provides more robust skill improvements in the root zone compared with underestimating the coupling. When CLM is excluded from the analysis, the skill improvements from using models with different vertical coupling strengths are comparable for different subsurface truths. Finally, the skill improvements through assimilation were found to be sensitive to the regional climate and soil types.

Effect of citric acid, tetracycline, and doxycycline on instrumented periodontally involved root surfaces: A SEM study

PubMed Central

Chahal, Gurparkash Singh; Chhina, Kamalpreet; Chhabra, Vipin; Bhatnagar, Rakhi; Chahal, Amna

2014-01-01

Background: A surface smear layer consisting of organic and inorganic material is formed on the root surface following mechanical instrumentation and may inhibit the formation of new connective tissue attachment to the root surface. Modification of the tooth surface by root conditioning has resulted in improved connective tissue attachment and has advanced the goal of reconstructive periodontal treatment. Aim: The aim of this study was to compare the effects of citric acid, tetracycline, and doxycycline on the instrumented periodontally involved root surfaces in vitro using a scanning electron microscope. Settings and Design: A total of 45 dentin samples obtained from 15 extracted, scaled, and root planed teeth were divided into three groups. Materials and Methods: The root conditioning agents were applied with cotton pellets using the Passive burnishing technique for 5 minutes. The samples were then examined by the scanning electron microscope. Statistical Analysis Used: The statistical analysis was carried out using Statistical Package for Social Sciences (SPSS Inc., Chicago, IL, version 15.0 for Windows). For all quantitative variables means and standard deviations were calculated and compared. For more than two groups ANOVA was applied. For multiple comparisons post hoc tests with Bonferroni correction was used. Results: Upon statistical analysis the root conditioning agents used in this study were found to be effective in removing the smear layer, uncovering and widening the dentin tubules and unmasking the dentin collagen matrix. Conclusion: Tetracycline HCl was found to be the best root conditioner among the three agents used. PMID:24744541

Clinical evaluation of demineralization and remineralization of intact root surface lesions in the clinic by a quantitative light-induced fluorescence system.

PubMed

Durmusoglu, Oykü; Tağtekin, Dilek Arslantunali; Yanikoğlu, Funda

2012-03-01

Detection of demineralization of root surface caries is an important issue since preventive approaches prolong tooth life. Quantitative light-induced fluorescence (QLF) has been shown to be useful for the laboratory assessment of demineralization of root surfaces. The aim of this study was to determine the demineralization and remineralization of root surface intact and cavitated caries lesions using a QLF system as a nondestructive in vivo method. Noncavitated and demineralized root surface lesions were detected and scored using the QLF system. Oral hygiene education was given and periodontal cleaning was completed before the remineralization treatment. After obtaining baseline QLF data, the patients were informed about the remineralization treatment. Fluoride varnish was applied to the carious lesions at the baseline visit, and the patients were then reviewed after 1, 2, 3 and 4 weeks, with QLF assessment and fluoride varnish application repeated at each review. Repeated-measures ANOVA (α = 0.05) showed significant differences between ΔQ values at each visit (p < 0.001); ΔQ showed marked decreases at all the cut-off values (15, 20, 25, 30). The changes in ΔQ were not affected by the cut-off value. The ΔQ values of QLF showed differences at all visits. The QLF system was able to detect early root surface caries lesions in vivo. Bifluoride 12 varnish improved mineral levels as shown by the QLF system. The treatment response to chemicals of intact noncavitated root surface carious lesions could be followed nondestructively in the clinic using QLF to quantify remineralization at recall visits. Teeth with root surface caries can be kept by controlling their remineralization.

Micro-computed tomography evaluation of the preparation of mesiobuccal root canals in maxillary first molars with Hyflex CM, Twisted Files, and K3 instruments.

PubMed

Zhao, Dan; Shen, Ya; Peng, Bin; Haapasalo, Markus

2013-03-01

The aim of this study was to describe the canal shaping properties of Hyflex CM, Twisted Files (TF), and K3 rotary nickel-titanium files by using micro-computed tomography in maxillary first molars. A total of 36 mesiobuccal root canals of maxillary first molars were prepared with Hyflex CM, TF, or K3 system. Micro-computed tomography was used to scan the specimens before and after instrumentation. The volume of untreated canal, volume of dentin removed after preparation, amount of uninstrumented area, and the transportation for the coronal, middle, and apical thirds of canals were measured. Instrumentation of canals increased their volume and surface area. TF group showed the greatest amount of volumetric dentin removal (P < .05), whereas no significant difference was found in Hyflex CM and K3 groups. There were no significant differences among instrument types concerning uninstrumented area. The TF system produced significantly less transportation than the K3 system in the apical third of canals. No significant difference was found between TF and Hyflex CM instruments relating to apical transportation. In vitro, Hyflex and TF instruments shaped curved root canals in maxillary first molar without significant shaping errors. Crown Copyright © 2013. Published by Elsevier Inc. All rights reserved.

Apical root resorption during orthodontic treatment. A prospective study using cone beam CT.

PubMed

Lund, Henrik; Gröndahl, Kerstin; Hansen, Ken; Gröndahl, Hans-Göran

2012-05-01

To investigate the incidence and severity of root resorption during orthodontic treatment by means of cone beam computed tomography (CBCT) and to explore factors affecting orthodontically induced inflammatory root resorption (OIIRR). CBCT examinations were performed on 152 patients with Class I malocclusion. All roots from incisors to first molars were assessed on two or three occasions. At treatment end, 94% of patients had ≥1 root with shortening >1 mm, and 6.6% had ≥1 tooth where it exceeded 4 mm. Among teeth, 56.3% of upper lateral incisors had root shortening >1 mm. Of upper incisors and the palatal root of upper premolars, 2.6% showed root shortenings >4 mm. Slanted surface resorptions of buccal and palatal surfaces were found in 15.1% of upper central and 11.5% of lateral incisors. Monthly root shortening was greater after 6-month control than before. Upper jaw teeth and anterior teeth were significantly associated with the degree of root shortening. Gender, root length at baseline, and treatment duration were not. Practically all patients and up to 91% of all teeth showed some degree of root shortening, but few patients and teeth had root shortenings >4 mm. Slanted root resorption was found on root surfaces that could be evaluated only by a tomographic technique. A CBCT technique can provide more valid and accurate information about root resorption.

Comparison of Quick-Set and mineral trioxide aggregate root-end fillings for the regeneration of apical tissues in dogs.

PubMed

Kohout, George D; He, Jianing; Primus, Carolyn M; Opperman, Lynne A; Woodmansey, Karl F

2015-02-01

Quick-Set (Avalon Biomed Inc, Bradenton, FL) is a calcium aluminosilicate cement that is a potential alternative to mineral trioxide aggregate (MTA) with greater acid resistance and faster setting. The purpose of this study was to compare the regeneration of apical tissues after root-end surgery when the apical tissues were exposed to Quick-Set or White ProRoot MTA (Dentsply Tulsa Dental Specialties, Tulsa, OK) by root-end resection. The root canals of 42 mandibular premolars in 7 beagle dogs were accessed, cleaned and shaped, and obturated with Quick-Set or white MTA. Osteotomies and root-end resections were performed immediately. The dogs were sacrificed at 90 days, and the teeth and surrounding tissues were removed and prepared for histologic analysis. The sections of the apical areas were scored for inflammation, new cementum formation, periodontal ligament formation, and bone quality. At 90 days, both materials supported some degree of cementum formation on the surface of the material, periodontal ligament regeneration, and excellent bone quality. The only significant difference was greater inflammation found in the Quick-Set group. Quick-Set and White ProRoot MTA had a similar effect on bone quality, cementum formation, and periodontal ligament formation after root-end surgery in dogs. Quick-Set was associated with greater inflammation. Copyright © 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Costs of jasmonic acid induced defense in aboveground and belowground parts of corn (Zea mays L.).

PubMed

Feng, Yuanjiao; Wang, Jianwu; Luo, Shiming; Fan, Huizhi; Jin, Qiong

2012-08-01

Costs of jasmonic acid (JA) induced plant defense have gained increasing attention. In this study, JA was applied continuously to the aboveground (AG) or belowground (BG) parts, or AG plus BG parts of corn (Zea mays L.) to investigate whether JA exposure in one part of the plant would affect defense responses in another part, and whether or not JA induced defense would incur allocation costs. The results indicated that continuous JA application to AG parts systemically affected the quantities of defense chemicals in the roots, and vice versa. Quantities of DIMBOA and total amounts of phenolic compounds in leaves or roots generally increased 2 or 4 wk after the JA treatment to different plant parts. In the first 2 wk after application, the increase of defense chemicals in leaves and roots was accompanied by a significant decrease of root length, root surface area, and root biomass. Four weeks after the JA application, however, no such costs for the increase of defense chemicals in leaves and roots were detected. Instead, shoot biomass and root biomass increased. The results suggest that JA as a defense signal can be transferred from AG parts to BG parts of corn, and vice versa. Costs for induced defense elicited by continuous JA application were found in the early 2 wk, while distinct benefits were observed later, i.e., 4 wk after JA treatment.

Periodontal tissue repair after sealing of the gap in vertical root fracture.

PubMed

Sugaya, Tsutomu; Tomita, Mahito; Motoki, Youji; Zaman, Khurshiduz; Miyaji, Hirofumi; Kawanami, Masamitsu

2017-04-01

The aim of this study was to determine whether sealing of fracture gap using adhesive resin through the root canal can prevent inflammation of periodontal tissue, and resealing the incompletely sealed fracture gap from outside can resolve such inflammation in experimentally created vertical root fractures. Vertical root fractures were created in incisor of beagles. In the experimental group, the fracture gap was sealed through the root canal with adhesive resin. After 5 weeks, sites with the clinical attachment level ≥4 mm were further divided randomly into the poor-replanting group and the poor-untreated group. In the poor-replanting group, the tooth was extracted and replanted after resealing the fracture gap with adhesive resin from the outer surface. Sites with clinical attachment level ≤3 mm after 5 weeks were considered as the satisfactory group. The poor-untreated group and the satisfactory group were subjected to no further treatment. The clinical attachment level was evaluated at baseline and after 2, 5, and 9 weeks. After 9 weeks, histological measurements were made to determine the length of the epithelial downgrowth and the area of alveolar bone resorption. The clinical attachment level and the area of bone resorption were significantly smaller in the poor-replanting group and the satisfactory group than in the poor-untreated group (p < 0.05). The results indicate the possibility that periodontal inflammation along the fracture line can be prevented and improved if the fracture gap is sealed.

Root tensile strength of grey alder and mountain maple grown on a coarse grained eco-engineered slope in the Swiss Alps related to wood anatomical features

NASA Astrophysics Data System (ADS)

Kink, Dimitri; Bast, Alexander; Meyer, Christine; Meier, Wolfgang; Egli, Markus; Gärtner, Holger

2014-05-01

Steep, vegetation free slopes are a common feature in alpine areas. The material covering these slopes is prone to all kind of erosional processes, resulting in a high risk potential for population and infrastructure. This risk potential is likely to increase with the predicted change in the spatiotemporal distribution of precipitation events. A potential increase in extreme precipitation events will also result in a higher magnitude and frequency of erosional processes. In the Swiss Alps as in many other mountainous areas, there is a need to stabilize these slopes to reduce their direct or indirect hazard potential. In this regard, eco-engineering is a very promising and sustainable approach for slope stabilization. Planting trees and shrubs is a central task in eco-engineering. A developing vegetation cover will on one hand reduce the mechanical effects of rainfall by an increased interception, on the other hand, the root systems cause modifications of soil properties. Roots not only provide anchorage for the plants, they also promote soil aggregation and are able to penetrate possible shear horizons. Overall, anchorage of plants is at the same extend also stabilizing the near subsurface. When rainfall occurs, the saturated soil exerts downhill pressure to a tree or shrub. As long as the root distribution supports anchorage, the respective slope area remains stable. At this point, the tensile strength of the roots is a critical measure, because it is more likely that the supporting roots break than the entire root system being pulled out of the soil completely. As a consequence, root tensile strength is an important parameter in characterizing the soil stabilization potential of trees and shrubs. It is known that tree roots show a high variability in their anatomical structure depending on their depth below soil surface as well as their distance to the main stem. Therefore, we assume that these structural changes affect the tensile strength of every single root. In order to confirm this assumption and possibly find more important root properties which have an influence on soil stabilization, the root systems of seven trees (three grey alder, four mountain maple) were excavated and analyzed. The study site is a catchment, where shallow landslides are common. It is located in the Prättigau valley in the Eastern Swiss Alps and was eco-engineered in 1997. The substrate is coarse-grained morainic material, mean annual air temperature reaches 4.64°C, average precipitation is 1170 mm, and the altitude is about 1000 m a.s.l.. The root system of each tree was uncovered carefully by hand to keep the roots undamaged, before removal it was photographed in situ to document the root distribution. The root systems were then cut into single root pieces of about 20 cm length and the position of each sample was documented. The root samples were then hierarchically classified in several root classes. The tensile strength of more than 500 samples was determined. In addition, the values for age, diameter, and root moisture were ascertained. Since it was assumed, that the cellular structure of the roots has an influence on the tensile strength, two microscopic thin-sections were prepared from all successfully tested root samples. The microscopic analysis focused on anatomical parameters such as the size and number of vessels, their distribution as well as their conductivity. The results for the final correlation between the anatomical characteristics and the root's tensile strength are presented for both tree species.

Effect of surface treatment of fiberglass posts on bond strength to root dentin.

PubMed

Valdivia, Andréa Dolores Correia Miranda; Novais, Veridiana Resende; Menezes, Murilo de Sousa; Roscoe, Marina Guimarães; Estrela, Carlos; Soares, Carlos José

2014-01-01

This study evaluated the influence of the surface treatments of fiberglass posts on bond strength to root dentin using push-out test. Forty bovine incisor roots were endodontically treated. The surface of the fiberglass posts (Exacto #2, Angelus) were treated using 4 different protocols (n=10): Control - 70% ethanol for 1 min; 37% phosphoric acid for 1 min; 10% hydrofluoric acid for 1 min; and 24% hydrogen peroxide for 1 min. After a silane coupling agent was applied for 1 min and all posts were cemented using self-adhesive resin cement (RelyX Unicem, 3M-ESPE). The roots were sectioned and two 1-mm-thick slices were obtained from each third: cervical, middle and apical. The specimens were subjected to the push-out test with a crosshead speed of 0.5 mm/min. Data were analyzed by repeated measures ANOVA followed by Tukey's HSD tests (=0.05). The surface treatment (p<0.001) and root third region (p=0.007) factors were significant. The retention to root canal was affected by surface treatment type. The post surface treatment with 24% hydrogen peroxide for 1 min yielded significantly higher bond strength when the fiberglass posts were cemented with RelyX Unicem.

Molecular analysis of SCARECROW genes expressed in white lupin cluster roots

PubMed Central

Sbabou, Laila; Bucciarelli, Bruna; Miller, Susan; Liu, Junqi; Berhada, Fatiha; Filali-Maltouf, Abdelkarim; Allan, Deborah; Vance, Carroll

2010-01-01

The Scarecrow (SCR) transcription factor plays a crucial role in root cell radial patterning and is required for maintenance of the quiescent centre and differentiation of the endodermis. In response to phosphorus (P) deficiency, white lupin (Lupinus albus L.) root surface area increases some 50-fold to 70-fold due to the development of cluster (proteoid) roots. Previously it was reported that SCR-like expressed sequence tags (ESTs) were expressed during early cluster root development. Here the cloning of two white lupin SCR genes, LaSCR1 and LaSCR2, is reported. The predicted amino acid sequences of both LaSCR gene products are highly similar to AtSCR and contain C-terminal conserved GRAS family domains. LaSCR1 and LaSCR2 transcript accumulation localized to the endodermis of both normal and cluster roots as shown by in situ hybridization and gene promoter::reporter staining. Transcript analysis as evaluated by quantitative real-time-PCR (qRT-PCR) and RNA gel hybridization indicated that the two LaSCR genes are expressed predominantly in roots. Expression of LaSCR genes was not directly responsive to the P status of the plant but was a function of cluster root development. Suppression of LaSCR1 in transformed roots of lupin and Medicago via RNAi (RNA interference) delivered through Agrobacterium rhizogenes resulted in decreased root numbers, reflecting the potential role of LaSCR1 in maintaining root growth in these species. The results suggest that the functional orthologues of AtSCR have been characterized. PMID:20167612

Application of paclobutrazol affect maize grain yield by regulating root morphological and physiological characteristics under a semi-arid region.

PubMed

Kamran, Muhammad; Wennan, Su; Ahmad, Irshad; Xiangping, Meng; Wenwen, Cui; Xudong, Zhang; Siwei, Mou; Khan, Aaqil; Qingfang, Han; Tiening, Liu

2018-03-19

A field experiment was conducted to investigate the effects of paclobutrazol on ear characteristics and grain yield by regulating root growth and root-bleeding sap of maize crop. Seed-soaking at rate of 0 (CK1), 200 (S1), 300 (S2), and 400 (S3) mg L -1 , and seed-dressing at rate of 0 (CK2), 1.5 (D1), 2.5 (D2), and 3.5 (D3) g kg -1 were used. Our results showed that paclobutrazol improved the ear characteristics and grain yield, and were consistently higher than control during 2015-2016. The average grain yield of S1, S2 and S3 were 18.9%, 61.3%, and 45.9% higher, while for D1, D2 and D3 were 20.2%, 33.3%, and 45.2%, compared to CK, respectively. Moreover, paclobutrazol-treated maize had improved root-length density (RLD), root-surface area density (RSD) and root-weight density (RWD) at most of the soil profiles (0-70 cm for seed-soaking, 0-60 cm for seed-dressing) and was attributed to enhancing the grain yield. In addition, root-activity, root-bleeding sap, root dry weight, diameter and root/shoot ratio increased by paclobutrazol, with highest values achieved in S2 and D3 treatments, across the whole growth stages in 2015-2016. Our results suggested that paclobutrazol could efficiently be used to enhance root-physiological and morphological characteristics, resulting in higher grain yield.

Effect of lipid/polysaccharide ratio on surface activity of model root mucilage in its solid and liquid states

NASA Astrophysics Data System (ADS)

Chen, Fengxian; Arye, Gilboa

2016-04-01

The rhizosphere can be defined as the volume of soil around living roots, which is influenced by root activity. The biological, chemical and physical conditions that prevail in the rhizosphere are significantly different from those of the bulk soil. Plant roots can release diverse organic materials in the rhizosphere which may have different effects on its bio-chemo-physical activity. Among these exudates is the root mucilage which can play a role on the maintenance of root-soil contact, lubrication of the root tip, protection of roots from desiccation and disease, stabilization of soil micro-aggregates and the selective absorption and storage of ions. The surface activity of the root mucilage at the liquid-air interface deduced from its surface tension depression relative to water, implying on its amphiphilic nature. Consequently as the rhizosphere dry out, hydrophobic functional groups may exhibit orientation at the solid-air interface and thus, the wettability of the rhizosphere may temporarily decrease. The major fraction of the root mucilage comprise of polysaccharides and to a much lesser extent, amino acids, organic acids, and phospholipids. The most frequent polysaccharide and phospholipids detected in root mucilage are polygalacturonic acid (PGA) and Phosphatidylcholine (PC), respectively. The latter, is thought to be main cause for the surface active nature of root mucilage. Nevertheless, the role and function of root mucilage in the rhizosphere is commonly studied based on model root mucilage that comprise of only one component, where the most frequent ones are PGA or PC (or lecithin). The main objective of this study was to quantify the effect of concentration and PGA/PC ratios on the wettability of a model rhizosphere soil and the surface tension of the model root mucilage at the liquid-air interface. The PGA/PC mixtures were measured for their equilibrium and dynamic surface tension using the Wilhelmy-Plate method. Quartz sand or glass slides were coated with PGA and/or PC using the above solutions and measured for their initial advancing contact angle and dynamic one, using the capillary rise and sessile drop methods, respectively. The results of this study will be presented and their implications for the wettability of the rhizosphere will be discussed.

Applications and Engineering Analysis of Lotus Roots under External Water Pressure

PubMed Central

Wang, Chang Jiang; Mynors, Diane

2016-01-01

Engineers can learn from nature for inspirations to create new designs. The internal structure of lotus roots with several oval holes was studied in this paper for engineering inspirations. The structural performance of lotus roots under outside water pressure was simulated and compared with various cross-sectional areas. The distribution of stresses in the cross-sectional area of lotus roots was analysed and presented. It was found that the maximum compressive stresses in the cross-sectional area of lotus roots were occurring at the long axis ends of the holes. This was very different from that of circular holes. Further analysis on the triaxiality factors revealed that the cross-sectional area of the lotus root resulted in large areas of high triaxiality factors. The resulting hydrostatic stress in the cross-sectional area of lotus root ranges from zero to 2.7 times the applied outside pressure. In contrast, the hydrostatic stress in a cylindrical cross-sectional area is a fixed value. The study showed that the lotus root and the orientation of the oval holes could be mimicked in the design of new structures, for example, underwater pipes and vessels. PMID:28127228

Shaping ability of single-file reciprocating and heat-treated multifile rotary systems: a micro-CT study.

PubMed

Marceliano-Alves, M F V; Sousa-Neto, M D; Fidel, S R; Steier, L; Robinson, J P; Pécora, J D; Versiani, M A

2015-12-01

To investigate changes in three-dimensional geometry, in various cross-sectional morphological parameters and in the centring ability of root canals prepared with different preparation systems using microcomputed tomographic imaging technology. Sixty-four mesial canals of mandibular molars were matched based on similar morphological dimensions using micro-CT evaluation and assigned to four experimental groups (n = 16), according to the canal preparation technique: Reciproc, WaveOne, Twisted File and HyFlex CM systems. Changes in several 2D (area, perimeter, form factor, roundness, minor and major diameter) and 3D [volume, surface area, structure model index (SMI)] morphological parameters, as well as canal transportation, were compared with preoperative values using Kruskal-Wallis and anovapost hoc Tukey's tests with the significance level set at 5%. Preparation significantly increased all tested parameters in the experimental groups. No significant differences were observed between groups regarding changes in volume, surface area, SMI, form factor and roundness of the root canal after preparation (P > 0.05). In the apical third, the Reciproc group had significantly greater changes in canal area, perimeter, major and minor diameters than the other groups (P < 0.05). Overall, the Twisted File and HyFlex CM systems were associated with significantly less transportation than the reciprocating instruments, Reciproc and WaveOne (P < 0.05). Shaping procedures led to the enlargement of the root canal space with no evidence of significant preparation errors. Changes in 3D parameters were not different between groups whilst, in the apical third, Reciproc was associated with significantly greater changes in several 2D parameters compared to the other groups. Twisted File and HyFlex CM systems were able to maintain the original canal anatomy with less canal transportation than Reciproc and WaveOne; however, these differences are unlikely to be of clinical significance. © 2014 International Endodontic Journal. Published by John Wiley & Sons Ltd.

In Vitro Adhesion of Streptococcus sanguinis to Dentine Root Surface After Treatment with Er:Yag Laser, Ultrasonic System, or Manual Curette

PubMed Central

Martins, Fernanda L.; Giorgetti, Ana Paula O.; de Freitas, Patrícia M.; Duarte, Poliana M.

2009-01-01

Abstract Objective: The purpose of this in vitro study was to evaluate the dentine root surface roughness and the adherence of Streptococcus sanguinis (ATCC 10556) after treatment with an ultrasonic system, Er:YAG laser, or manual curette. Background Data: Bacterial adhesion and formation of dental biofilm after scaling and root planing may be a challenge to the long-term stability of periodontal therapy. Materials and Methods: Forty flattened bovine roots were randomly assigned to one of the following groups: ultrasonic system (n = 10); Er:YAG laser (n = 10); manual curette (n = 10); or control untreated roots (n = 10). The mean surface roughness (Ra, μm) of the specimens before and after exposure to each treatment was determined using a surface profilometer. In addition, S. sanguinis was grown on the treated and untreated specimens and the amounts of retained bacteria on the surfaces were measured by culture method. Results: All treatments increased the Ra; however, the roughest surface was produced by the curettes. In addition, the specimens treated with curettes showed the highest S. sanguinis adhesion. There was a significant positive correlation between roughness values and bacterial cells counts. Conclusion: S. sanguinis adhesion was the highest on the curette-treated dentine root surfaces, which also presented the greatest surface roughness. PMID:19712018

Monitoring soil moisture patterns in alpine meadows using ground sensor networks and remote sensing techniques

NASA Astrophysics Data System (ADS)

Bertoldi, Giacomo; Brenner, Johannes; Notarnicola, Claudia; Greifeneder, Felix; Nicolini, Irene; Della Chiesa, Stefano; Niedrist, Georg; Tappeiner, Ulrike

2015-04-01

Soil moisture content (SMC) is a key factor for numerous processes, including runoff generation, groundwater recharge, evapotranspiration, soil respiration, and biological productivity. Understanding the controls on the spatial and temporal variability of SMC in mountain catchments is an essential step towards improving quantitative predictions of catchment hydrological processes and related ecosystem services. The interacting influences of precipitation, soil properties, vegetation, and topography on SMC and the influence of SMC patterns on runoff generation processes have been extensively investigated (Vereecken et al., 2014). However, in mountain areas, obtaining reliable SMC estimations is still challenging, because of the high variability in topography, soil and vegetation properties. In the last few years, there has been an increasing interest in the estimation of surface SMC at local scales. On the one hand, low cost wireless sensor networks provide high-resolution SMC time series. On the other hand, active remote sensing microwave techniques, such as Synthetic Aperture Radars (SARs), show promising results (Bertoldi et al. 2014). As these data provide continuous coverage of large spatial extents with high spatial resolution (10-20 m), they are particularly in demand for mountain areas. However, there are still limitations related to the fact that the SAR signal can penetrate only a few centimeters in the soil. Moreover, the signal is strongly influenced by vegetation, surface roughness and topography. In this contribution, we analyse the spatial and temporal dynamics of surface and root-zone SMC (2.5 - 5 - 25 cm depth) of alpine meadows and pastures in the Long Term Ecological Research (LTER) Area Mazia Valley (South Tyrol - Italy) with different techniques: (I) a network of 18 stations; (II) field campaigns with mobile ground sensors; (III) 20-m resolution RADARSAT2 SAR images; (IV) numerical simulations using the GEOtop hydrological model (Rigon et al., 2006; Endrizzi et al., 2014). The objective of this work is to understand the physical controls of the observed SCM patterns. In particular, we want to investigate: • How the SMC signal propagates with depth, to understand the capability of SAR surface SMC observations to predict root-zone SMC. • The role of land management and vegetation properties with respect to soil and bedrock properties in determining SMC spatial variability and temporal patterns. In this context, we use the GEOtop model to understand if a relationship exists between the observed SMC patterns and the underlying runoff generation processes. Results show that meadows and pastures have different behaviours. Meadows are in general wetter because of irrigation and the presence of soils with higher organic content and higher water holding capacity. Moreover, surface and root depth SCM dynamics are correlated. In contrast, pastures are drier, with lower vegetation density and more compact soils due animal trampling. Because of shallow soils and impermeable bedrock, root zone SMC shows a different behaviour with respect to the surface, with occurrence of sub-surface saturation excess, as verified from numerical experiments performed with the hydrological model. Results suggest how SAR retrieved surface SMC can be used to extrapolate root zone SMC, when soil properties are homogenous and differences in vegetation density are properly accounted with a robust retrieval processes (Pasolli et al., in press 2015). However, in situations characterized by shallow subsurface saturation excess flow, a more sophisticated modelling approach is required to estimate root zone SMC using remote sensing observations. Bertoldi, G., Della, S., Notarnicola, C., Pasolli, L., Niedrist, G., & Tappeiner, U. (2014). Estimation of soil moisture patterns in mountain grasslands by means of SAR RADARSAT2 images and hydrological modeling, 516, 245-257. doi:10.1016/j.jhydrol.2014.02.018 Endrizzi, S., Gruber, S., Dall'Amico, M., & Rigon, R. (2014). GEOtop 2.0: simulating the combined energy and water balance at and below the land surface accounting for soil freezing, snow cover and terrain effects. Geoscientific Model Development, 7(6), 2831-2857. doi:10.5194/gmd-7-2831-2014 Pasolli, L., Notarnicola, C., Bertoldi, G., Bruzzone, L., Remegaldo, R., Niedrist, G, Della Chiesa S., Tappeiner, U., Zebisch, M. (2014): Multi-scale assessment of soil moisture variability in mountain areas by using active radar images. IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing, in press 2015. Rigon, R., Bertoldi, G., & Over, T. M. (2006). GEOtop: A Distributed Hydrological Model with Coupled Water and Energy Budgets. Journal of Hydrometeorology, 7, 371-388. Vereecken, H., Huisman, J. A., Pachepsky, Y., Montzka, C., van der Kruk, J., Bogena, H., … Vanderborght, J. (2014). On the spatio-temporal dynamics of soil moisture at the field scale. Journal of Hydrology. doi:http://dx.doi.org/10.1016/j.jhydrol.2013.11.061

The relationship between purely stochastic sampling error and the number of technical replicates used to estimate concentration at an extreme dilution

USDA-ARS?s Scientific Manuscript database

For any analytical system the population mean (mu) number of entities (e.g., cells or molecules) per tested volume, surface area, or mass also defines the population standard deviation (sigma = square root of mu ). For a preponderance of analytical methods, sigma is very small relative to mu due to...

The effect of MTAD, an endodontic irrigant, on fibroblast attachment to periodontally affected root surfaces: A SEM analysis

PubMed Central

Ghandi, Mostafa; Houshmand, Behzad; Nekoofar, Mohammad H.; Tabor, Rachel K.; Yadeghari, Zahra; Dummer, Paul M. H.

2013-01-01

Background: Root surface debridement (RSD) is necessary to create an environment suitable for reattachment of the periodontium. Root surface conditioning may aid the formation of a biocompatible surface suitable for cell reattachment. BioPure™ MTAD (mixture of Doxycycline, citric acid and a detergent) is an endodontic irrigant with antibacterial properties and the ability to remove smear layer. It was hypothesized that MTAD may be useful for root surface conditioning. The efficacy of MTAD as a conditioner was measured by examining fibroblast attachment to root surfaces. Materials and Methods: Thirty-two specimens of human teeth with advanced periodontal disease were used. The surfaces were root planed until smooth. Half of the specimens were treated with 0.9% saline and the other samples with Biopure MTAD. As a negative control group, five further samples were left unscaled with surface calculus. Human gingival fibroblast cells HGF1-PI1 were cultured and poured over the tooth specimens and incubated. After fixation, the samples were sputter-coated with gold and examined with a SEM. The morphology and number of attached, fixed viable cells were examined. The data was analysed using the Mann-Whitney-U statistical test. Results: There was no significant difference between the numbers of attached cells in the experimental group treated with MTAD and the control group treated with saline. Little or no attached cells were seen in the negative control group. Conclusion: RSD created an environment suitable for cell growth and attachment in a laboratory setting. The use of MTAD did not promote the attachment and growth of cells on the surface of human roots following RSD. PMID:23869124

Finite element analysis (FEA) of dental implant fixture for mechanical stability and rapid osseointegration

NASA Astrophysics Data System (ADS)

Tabassum, Shafia; Murtaza, Ahmar; Ali, Hasan; Uddin, Zia Mohy; Zehra, Syedah Sadaf

2017-10-01

For rapid osseointegration of dental implant fixtures, various surface treatments including plasma spraying, hydroxyapatite coating, acid-etching, and surface grooving are used. However undesirable effects such as chemical modifications, loss of mechanical properties, prolonged processing times and post production treatment steps are often associated with these techniques. The osseointegration rate of the dental implants can be promoted by increasing the surface area of the dental implant, thus increasing the bone cells - implant material contact and allow bone tissues to grow rapidly. Additive Manufacturing (AM) techniques can be used to fabricate dental implant fixtures with desirable surface area in a single step manufacturing process. AM allows the use of Computer Aided Designing (CAD) for customised rapid prototyping of components with precise control over geometry. In this study, the dental implant fixture that replaces the tooth root was designed on commercially available software COMSOL. Nickel - titanium alloy was selected as build materials for dental implant. The geometry of the dental fixture was varied by changing the interspacing distance (thread pitch) and number of threads to increase the total surface area. Three different microstructures were introduced on the surface of dental implant. The designed models were used to examine the effect of changing geometries on the total surface area. Finite Element Analysis (FEA) was performed to investigate the effect of changing geometries on the mechanical properties of the dental implant fixtures using stress analysis.

Saliva with reduced calcium and phosphorous concentrations: Effect on erosion dental lesions.

PubMed

Denucci, Giovanna Corrêa; Mantilla, Taís Fonseca; Amaral, Flávia Lucisano Botelho; Basting, Roberta Tarkany; França, Fabiana Mantovani Gomes; Turssi, Cecilia Pedroso

2018-02-08

To investigate whether saliva formulations with reduced calcium (Ca) and inorganic phosphorous (Pi) concentration would affect dental erosion caused by hydrochloric acid (HCl). Enamel and root dentine bovine slabs were embedded, polished and measured for surface Knoop microhardness (SMH). After reference areas were created, specimens were exposed to HCl solution (0.01M; pH 2; 120s) and immersed in artificial salivas (6h) containing three different Ca/Pi concentrations (n=15), which simulate serum conditions of normo-, mild- or severe hypocalcaemia. The control group was immersed in Ca/Pi-free saliva. The study protocol was carried out 2x/day for 5 days. Surface loss of enamel and root dentine was assessed using an optical profilometer and SMH was remeasured for enamel. ANOVA (p<0.001) and Tukey's test showed that enamel loss in groups subjected to artificial salivas that simulated mild- or severe hypocalcaemia did not differ from that resembling normocalcemia. %SMH was lower when saliva was mildly- and normally-concentrated in Ca/Pi (p<0.001). Root dentine loss was higher in saliva simulating severe hypocalcaemia than in those referring to mild, hypo- and normocalcemia. Depending on the dental substrate, salivary formulations resembling serum hypocalcaemia affected surface loss due to erosion and rehardening thereof. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Implementing Dynamic Root Optimization in Noah-MP for Simulating Phreatophytic Root Water Uptake

NASA Astrophysics Data System (ADS)

Wang, Ping; Niu, Guo-Yue; Fang, Yuan-Hao; Wu, Run-Jian; Yu, Jing-Jie; Yuan, Guo-Fu; Pozdniakov, Sergey P.; Scott, Russell L.

2018-03-01

Widely distributed in arid and semiarid regions, phreatophytic roots extend into the saturated zone and extract water directly from groundwater. In this paper, we implemented a vegetation optimality model of root dynamics (VOM-ROOT) in the Noah land surface model with multiparameterization options (Noah-MP LSM) to model the extraction of groundwater through phreatophytic roots at a riparian site with a hyperarid climate (with precipitation of 35 mm/yr) in northwestern China. VOM-ROOT numerically describes the natural optimization of the root profile in response to changes in subsurface water conditions. The coupled Noah-MP/VOM-ROOT model substantially improves the simulation of surface energy and water fluxes, particularly during the growing season, compared to the prescribed static root profile in the default Noah-MP. In the coupled model, more roots are required to grow into the saturated zone to meet transpiration demand when the groundwater level declines over the growing season. The modeling results indicate that at the study site, the modeled annual transpiration is 472 mm, accounting for 92.3% of the total evapotranspiration. Direct root water uptake from the capillary fringe and groundwater, which is supplied by lateral groundwater flow, accounts for approximately 84% of the total transpiration. This study demonstrates the importance of implementing a dynamic root scheme in a land surface model for adequately simulating phreatophytic root water uptake and the associated latent heat flux.

Correlative bacteriologic and micro-computed tomographic analysis of mandibular molar mesial canals prepared by self-adjusting file, reciproc, and twisted file systems.

PubMed

Siqueira, José F; Alves, Flávio R F; Versiani, Marco A; Rôças, Isabela N; Almeida, Bernardo M; Neves, Mônica A S; Sousa-Neto, Manoel D

2013-08-01

This ex vivo study evaluated the disinfecting and shaping ability of 3 protocols used in the preparation of mesial root canals of mandibular molars by means of correlative bacteriologic and micro-computed tomographic (μμCT) analysis. The mesial canals of extracted mandibular molars were contaminated with Enterococcus faecalis for 30 days and assigned to 3 groups based on their anatomic configuration as determined by μCT analysis according to the preparation technique (Self-Adjusting File [ReDent-Nova, Ra'anana, Israel], Reciproc [VDW, Munich, Germany], and Twisted File [SybronEndo, Orange, CA]). In all groups, 2.5% NaOCl was the irrigant. Canal samples were taken before (S1) and after instrumentation (S2), and bacterial quantification was performed using culture. Next, mesial roots were subjected to additional μCT analysis in order to evaluate shaping of the canals. All instrumentation protocols promoted a highly significant intracanal bacterial reduction (P < .001). Intergroup quantitative and qualitative comparisons disclosed no significant differences between groups (P > .05). As for shaping, no statistical difference was observed between the techniques regarding the mean percentage of volume increase, the surface area increase, the unprepared surface area, and the relative unprepared surface area (P > .05). Correlative analysis showed no statistically significant relationship between bacterial reduction and the mean percentage increase of the analyzed parameters (P > .05). The 3 instrumentation systems have similar disinfecting and shaping performance in the preparation of mesial canals of mandibular molars. Copyright © 2013 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

The Regulation of Growth in the Distal Elongation Zone of Maize Roots

NASA Technical Reports Server (NTRS)

Evans, Michael L.

1998-01-01

The major goals of the proposed research were 1. To develop specialized software for automated whole surface root expansion analysis and to develop technology for controlled placement of surface electrodes for analysis of relationships between root growth and root pH and electrophysiological properties. 2. To measure surface pH patterns and determine the possible role of proton flux in gravitropic sensing or response, and 3. To determine the role of auxin transport in establishment of patterns of proton flux and electrical gradients during the gravitropic response of roots with special emphasis on the role of the distal elongation zone in the early phases of the gravitropic response.

Exploratory Project: Rigid nanostructured organic polymer monolith for in situ collection and analysis of plant metabolites from soil matrices

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tharayil, Nishanth

Plant metabolites released from litter leachates and root exudates enable plants to adapt and survive in a wide range of habitats by facilitating resource foraging and plant-organismal interactions, and could influence soil carbon storage. The biological functions of these plant inputs and the organismal interactions they facilitate in soil are strictly governed by their composition and molecular identity. Our current understanding about the molecular identity of exudates is based on physiological studies that are done in soil-less axenic cultures. On the other hand, ecological studies that rely on isotope labeling to track the fluxes of carbon from plants to soilmore » have found the complexities of soil-microbe matrices as an insurmountable barrier to undertake any meaningful molecular level characterization of plant inputs. Although it is constantly advocated to undertake a molecular level identification of the dynamicity of plant metabolites in soils, the complexity of soil system has thus far prevented any such endeavors. We developed polymeric probes through in-situ polymerization of poly(styrene-co-vinylbenzyl chloride-co-divinylbenzene) to elucidate the chemical environment of the soil to which the plant roots are exposed. Hypercrosslinking of the polymeric probes through an in-situ Friedel-Crafts alkylation significantly increased the surface area and the sorption capacity of the probes. Surface functionalization of the probes using a hybrid approach was also attempted. The efficacy of these probes was tested using batch equilibration. Scanning electron microscopy revealed extensive modification of the surface of the probes through hypercrosslinking. The probes exhibited a lower site specific sorption (slope of Freundlich adsorption isotherm close to unity) and percent recovery of the sorbed compounds from the probes were >70, indicating a predominance of reversible sorption. Further we imparted specificity to this copolymer matrix by using molecular-template imprinting approach for the selective capture of less abundant plant metabolites from a crowded soil/litter leachate. Our results suggests that i) the root exudate pattern of plants is highly dependent on the nutrient status of the plant, with greater specificity of root exudation occurring in growing medium with low available form of nutrients, ii) the chemical composition of root exudation is a function of the distance of sampling from the source-roots, with the composition of root exudation being more enriched in polar metabolites farther from the source-roots, iii) Further we demonstrated that the compounds present in real root exudates diffuse farther from the source roots than those in artificial root exudates that are traditionally used. Thus, our project highlights how the soil matrix is instrumental in modifying the chemical composition of root exudates, and highlights that, apart from the plant physiology, the specificity and function of root exudates is also modified by environmental factors.« less

Effects of increased apical enlargement on the amount of unprepared areas and coronal dentine removal: a micro-computed tomography study.

PubMed

Pérez, A R; Alves, F R F; Marceliano-Alves, M F; Provenzano, J C; Gonçalves, L S; Neves, A A; Siqueira, J F

2018-06-01

To evaluate the effects of progressive apical enlargement on the amount of unprepared root canal surface area and remaining dentine thickness. The root canals of 30 extracted mandibular incisors with Vertucci's type I configuration were instrumented with rotary HyFlex CM instruments (Coltene-Whaledent, Altstätten, Switzerland) up to 4 instruments larger than the first one that bound at the working length (WL). Teeth were scanned in a micro-computed tomography (micro-CT) device before canal preparation and after instrumentation with the 2nd, 3rd and 4th larger instruments. The amount of unprepared surface area in the full canal or in the apical 4 mm as well as the remaining dentine thickness at 10 mm from the WL were calculated and compared. The general linear model for repeated measures adjusted by Bonferroni's post hoc test was used for statistic analysis. There was a significant reduction in the amount of unprepared areas after each increase in preparation size (P < 0.01). This was observed for both the full canal length and the 4-mm apical segment. The amount of remaining dentine was also significantly reduced after each file size (P < 0.01). However, dentine thickness always remained greater than 1 mm, even after using the largest instrument. Apical preparations up to 4 instruments larger than the first one to bind at the WL caused a significant progressive reduction in the unprepared canal area. © 2017 International Endodontic Journal. Published by John Wiley & Sons Ltd.

Can we manipulate root system architecture to control soil erosion?

NASA Astrophysics Data System (ADS)

Ola, A.; Dodd, I. C.; Quinton, J. N.

2015-03-01

Soil erosion is a major threat to soil functioning. The use of vegetation to control erosion has long been a topic for research. Much of this research has focused on the above ground properties of plants, demonstrating the important role that canopy structure and cover plays in the reduction of water erosion processes. Less attention has been paid to plant roots. Plant roots are a crucial yet under-researched factor for reducing water erosion through their ability to alter soil properties, such as aggregate stability, hydraulic function and shear strength. However, there have been few attempts to manipulate plant root system properties to reduce soil erosion. Therefore, this review aims to explore the effects that plant roots have on soil erosion and hydrological processes, and how plant root architecture might be manipulated to enhance its erosion control properties. We clearly demonstrate the importance of root system architecture for the control of soil erosion. We also demonstrate that some plant species respond to nutrient enriched patches by increasing lateral root proliferation. The soil response to root proliferation will depend upon its location: at the soil surface dense mats of roots may block soil pores thereby limiting infiltration, enhancing runoff and thus erosion; whereas at depth local increases in shear strength may reinforce soils against structural failure at the shear plane. Additionally, in nutrient deprived regions, root hair development may be stimulated and larger amounts of root exudates released, thereby improving aggregate stability and decreasing erodibility. Utilising nutrient placement at depth may represent a potentially new, easily implemented, management strategy on nutrient poor agricultural land or constructed slopes to control erosion, and further research in this area is needed.

Identification of Quantitative Trait Loci Controlling Root and Shoot Traits Associated with Drought Tolerance in a Lentil (Lens culinaris Medik.) Recombinant Inbred Line Population

PubMed Central

Idrissi, Omar; Udupa, Sripada M.; De Keyser, Ellen; McGee, Rebecca J.; Coyne, Clarice J.; Saha, Gopesh C.; Muehlbauer, Fred J.; Van Damme, Patrick; De Riek, Jan

2016-01-01

Drought is one of the major abiotic stresses limiting lentil productivity in rainfed production systems. Specific rooting patterns can be associated with drought avoidance mechanisms that can be used in lentil breeding programs. In all, 252 co-dominant and dominant markers were used for Quantitative Trait Loci (QTL) analysis on 132 lentil recombinant inbred lines based on greenhouse experiments for root and shoot traits during two seasons under progressive drought-stressed conditions. Eighteen QTLs controlling a total of 14 root and shoot traits were identified. A QTL-hotspot genomic region related to a number of root and shoot characteristics associated with drought tolerance such as dry root biomass, root surface area, lateral root number, dry shoot biomass and shoot length was identified. Interestingly, a QTL (QRSratioIX-2.30) related to root-shoot ratio, an important trait for drought avoidance, explaining the highest phenotypic variance of 27.6 and 28.9% for the two consecutive seasons, respectively, was detected. This QTL was closed to the co-dominant SNP marker TP6337 and also flanked by the two SNP TP518 and TP1280. An important QTL (QLRNIII-98.64) related to lateral root number was found close to TP3371 and flanked by TP5093 and TP6072 SNP markers. Also, a QTL (QSRLIV-61.63) associated with specific root length was identified close to TP1873 and flanked by F7XEM6b SRAP marker and TP1035 SNP marker. These two QTLs were detected in both seasons. Our results could be used for marker-assisted selection in lentil breeding programs targeting root and shoot characteristics conferring drought avoidance as an efficient alternative to slow and labor-intensive conventional breeding methods. PMID:27602034

Temperature changes accompanying near infrared diode laser endodontic treatment of wet canals.

PubMed

Hmud, Raghad; Kahler, William A; Walsh, Laurence J

2010-05-01

Diode laser endodontic treatments such as disinfection or the generation of cavitations should not cause deleterious thermal changes in radicular dentin. This study assessed thermal changes in the root canal and on the root surface when using 940 and 980 nm lasers at settings of 4 W/10 Hz and 2.5 W/25 Hz, respectively, delivered into 2000-mum fibers to generate cavitations in water. The root surface temperature in the apical third was recorded, as was the water temperature in coronal, middle, and apical third regions, by using thermocouples placed inside the canal. Lasing was undertaken with either rest periods or rinsing between 5-second laser exposures. Both diode lasers induced only modest temperature changes on the external root surface at the settings used. Even though the temperature of the water within the canal increased during lasing by as much as 30 degrees C, the external root surface temperature increased by only a maximum of 4 degrees C. Irrigation between laser exposures was highly effective in minimizing thermal changes within the root canal and on the root surface. Diode laser parameters that induce cavitation do not result in adverse thermal changes in radicular dentin. Copyright (c) 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Neural Network-Based Retrieval of Surface and Root Zone Soil Moisture using Multi-Frequency Remotely-Sensed Observations

NASA Astrophysics Data System (ADS)

Hamed Alemohammad, Seyed; Kolassa, Jana; Prigent, Catherine; Aires, Filipe; Gentine, Pierre

2017-04-01

Knowledge of root zone soil moisture is essential in studying plant's response to different stress conditions since plant photosynthetic activity and transpiration rate are constrained by the water available through their roots. Current global root zone soil moisture estimates are based on either outputs from physical models constrained by observations, or assimilation of remotely-sensed microwave-based surface soil moisture estimates with physical model outputs. However, quality of these estimates are limited by the accuracy of the model representations of physical processes (such as radiative transfer, infiltration, percolation, and evapotranspiration) as well as errors in the estimates of the surface parameters. Additionally, statistical approaches provide an alternative efficient platform to develop root zone soil moisture retrieval algorithms from remotely-sensed observations. In this study, we present a new neural network based retrieval algorithm to estimate surface and root zone soil moisture from passive microwave observations of SMAP satellite (L-band) and AMSR2 instrument (X-band). SMAP early morning observations are ideal for surface soil moisture retrieval. AMSR2 mid-night observations are used here as an indicator of plant hydraulic properties that are related to root zone soil moisture. The combined observations from SMAP and AMSR2 together with other ancillary observations including the Solar-Induced Fluorescence (SIF) estimates from GOME-2 instrument provide necessary information to estimate surface and root zone soil moisture. The algorithm is applied to observations from the first 18 months of SMAP mission and retrievals are validated against in-situ observations and other global datasets.

Plant Growth Environments with Programmable Relative Humidity and Homogeneous Nutrient Availability

PubMed Central

Lind, Kara R.; Lee, Nigel; Sizmur, Tom; Siemianowski, Oskar; Van Bruggen, Shawn; Ganapathysubramaniam, Baskar

2016-01-01

We describe the design, characterization, and use of “programmable”, sterile growth environments for individual (or small sets of) plants. The specific relative humidities and nutrient availability experienced by the plant is established (RH between 15% and 95%; nutrient concentration as desired) during the setup of the growth environment, which takes about 5 minutes and <1$ in disposable cost. These systems maintain these environmental parameters constant for at least 14 days with minimal intervention (one minute every two days). The design is composed entirely of off-the-shelf components (e.g., LEGO® bricks) and is characterized by (i) a separation of root and shoot environment (which is physiologically relevant and facilitates imposing specific conditions on the root system, e.g., darkness), (ii) the development of the root system on a flat surface, where the root enjoys constant contact with nutrient solution and air, (iii) a compatibility with root phenotyping. We demonstrate phenotyping by characterizing root systems of Brassica rapa plants growing in different relative humidities (55%, 75%, and 95%). While most phenotypes were found to be sensitive to these environmental changes, a phenotype tightly associated with root system topology–the size distribution of the areas encircled by roots–appeared to be remarkably and counterintuitively insensitive to humidity changes. These setups combine many of the advantages of hydroponics conditions (e.g., root phenotyping, complete control over nutrient composition, scalability) and soil conditions (e.g., aeration of roots, shading of roots), while being comparable in cost and setup time to Magenta® boxes. PMID:27304431

SMAP Level 4 Surface and Root Zone Soil Moisture

NASA Technical Reports Server (NTRS)

Reichle, R.; De Lannoy, G.; Liu, Q.; Ardizzone, J.; Kimball, J.; Koster, R.

2017-01-01

The SMAP Level 4 soil moisture (L4_SM) product provides global estimates of surface and root zone soil moisture, along with other land surface variables and their error estimates. These estimates are obtained through assimilation of SMAP brightness temperature observations into the Goddard Earth Observing System (GEOS-5) land surface model. The L4_SM product is provided at 9 km spatial and 3-hourly temporal resolution and with about 2.5 day latency. The soil moisture and temperature estimates in the L4_SM product are validated against in situ observations. The L4_SM product meets the required target uncertainty of 0.04 m(exp. 3)m(exp. -3), measured in terms of unbiased root-mean-square-error, for both surface and root zone soil moisture.

Intracanal bisphosphonate does not inhibit replacement resorption associated with delayed replantation of monkey incisors.

PubMed

Thong, Yo Len; Messer, Harold H; Zain, Rosnah Binti; Saw, Lip Hean; Yoong, Lai Thong

2009-08-01

Progressive replacement resorption following delayed replantation of avulsed teeth has proved to be an intractable clinical problem. A wide variety of therapeutic approaches have failed to result in the predictable arrest of resorption, with a good long-term prognosis for tooth survival. Bisphosphonates are used in the medical management of a range of bone disorders and topically applied bisphosphonate has been reported to inhibit root resorption in dogs. This study evaluated the effectiveness of a bisphosphonate (etidronate disodium) as an intracanal medicament in the root canals of avulsed monkey teeth, placed before replantation after 1 h of extraoral dry storage. Incisors of six Macaca fascicularis monkeys were extracted and stored dry for 1 h. Teeth were then replanted after canal contamination with dental plaque (negative control) or after root canal debridement and placement of etidronate sealed in the canal space. A positive control of calcium hydroxide placed 8-9 days after replantation was also included. All monkeys were sacrificed 8 weeks later and block sections were prepared for histomorphometric assessment of root resorption and periodontal ligament status. Untreated teeth showed the greatest extent of root resorption (46% of the root surface), which was predominantly inflammatory in nature. Calcium hydroxide treated teeth showed the lowest overall level of resorption (<30% of the root surface), while the bisphosphonate-treated group was intermediate (39%). Ankylosis, defined as the extent of the root surface demonstrating direct bony union to both intact and resorbed root surface, was the lowest in the untreated control group (15% of the root surface), intermediate in the calcium hydroxide group (27%) and the highest in the bisphosphonate group (41%). Bony attachment to the tooth root was divided approximately equally between attachment to intact cementum and to previously resorbed dentin. Overall, bisphosphonate resulted in a worse outcome than calcium hydroxide in terms of both root resorption and ankylosis.

Rhizosphere biophysics and root water uptake

NASA Astrophysics Data System (ADS)

Carminati, Andrea; Zarebanadkouki, Mohsen; Ahmed, Mutez A.; Passioura, John

2016-04-01

The flow of water into the roots and the (putative) presence of a large resistance at the root-soil interface have attracted the attention of plant and soil scientists for decades. Such resistance has been attributed to a partial contact between roots and soil, large gradients in soil matric potential around the roots, or accumulation of solutes at the root surface creating a negative osmotic potential. Our hypothesis is that roots are capable of altering the biophysical properties of the soil around the roots, the rhizosphere, facilitating root water uptake in dry soils. In particular, we expect that root hairs and mucilage optimally connect the roots to the soil maintaining the hydraulic continuity across the rhizosphere. Using a pressure chamber apparatus we measured the relation between transpiration rate and the water potential difference between soil and leaf xylem during drying cycles in barley mutants with and without root hairs. The samples were grown in well structured soils. At low soil moistures and high transpiration rates, large drops in water potential developed around the roots. These drops in water potential recovered very slowly, even after transpiration was severely decreased. The drops in water potential were much bigger in barley mutants without root hairs. These mutants failed to sustain high transpiration rates in dry conditions. To explain the nature of such drops in water potential across the rhizosphere we performed high resolution neutron tomography of the rhizosphere of the barleys with and without root hairs growing in the same soil described above. The tomograms suggested that the hydraulic contact between the soil structures was the highest resistance for the water flow in dry conditions. The tomograms also indicate that root hairs and mucilage improved the hydraulic contact between roots and soil structures. At high transpiration rates and low water contents, roots extracted water from the rhizosphere, while the bulk soil, due its low unsaturated conductivity, failed to compensate root water uptake. We conclude that root hairs are functional to increase the contact area between the roots and the soil structures and mucilage maintains wet the soil region between root hairs. These observations demonstrate the importance of the biophysical processes in the rhizosphere in modulating root water uptake.

Coarse root topology of Norway spruce (Picea abies) and its effects on slope stability

NASA Astrophysics Data System (ADS)

Lith, Aniek; Schmaltz, Elmar; Bogaard, Thom; Keesstra, Saskia

2017-04-01

The structural distribution of coarse roots and its beneficial effects on soil reinforcement has widely been assessed. However, it is still not fully understood how topological features of coarse roots (e.g. branching patterns) are affected by slope inclination and further influence the ability of young trees to reinforce soil. This study aims to analyse empirically the impact of slope gradient on the topological development of coarse roots and thus to assess its effects on soil reinforcement. We performed root system excavations on two young Picea abies: tree A on a gently inclined plane (β ≈ 12°) where slope failures are not expected; tree B on a slope (β ≈ 35°) with failure potential. The diameter (d) of the segments between distinct root nodes (root ends, branching locations, direction changes and attachments to stem) of coarse roots (d > 2mm) were measured in situ. The spatial coordinates (x,y,z) of the nodes and surface were measured on a plane raster grid, from which segment length (ls), direction and inclination towards the surface (βr) were derived. Roots and segments were classified into laterals (βr < 10°), obliques (10° ≤ βr < 70°) and verticals (βr ≥ 70°), with βr,max = 90°. We assigned topological orders to the segments according to developmental (DSC) and functional segment classifications (FSC), to obtain quantitative relations between the topological order and number of segments, total and average ls. The maximal root cohesion (cr) of each segment was assessed using material specific tensile forces (Tr), root area ratio (RAR) and βr, assuming that a potential slip surface would cross the root system parallel to the slope. Laterals depicted the majority of roots (57 %) for tree A orientated rather in upslope direction (76.8 %), whereas tree B showed mostly obliques (54 %) orientated rather in downslope direction (55.4 %). Vertical roots were scarcely observable for both trees. DSC showed a high r2 (> 0.84) for the segments and ls. FSC showed high r2 (> 0.95) for the number of segments and the total length. RAR values of tree B are distributed rather upslope (76.8 % of RARtot), compared to 44.5 % of RARtot for tree A. The average cr (0.15) of each segment of tree B was remarkably higher than of tree A (0.10), leading to the conclusion that the slope has a strong influence on cr itself. This is supported by comparing the distribution of cr for both trees, where tree B tends to produce a higher cr in upslope direction (68.7 % of total cr) than tree A (37.7 %). In contrast to our expectations, tree B shows generally a higher cr compared to tree A, despite lower subsurface biomass. The findings indicate that the distinct branching patterns of coarse roots might determine the distribution of the RAR and thus lead to a higher reinforcement potential of young Picea abies on slopes.

Conductivity gradients as inferred by electromagnetic-induction meter (EM38) readings within a salt-affected wetland in Saskatchewan, Canada

NASA Astrophysics Data System (ADS)

Mirck, Jaconette; Schroeder, William

2018-01-01

The change from deep-rooted grass and shrub vegetation to annual-cropping dryland farming has contributed to serious soil salinization challenges on the semi-arid North American Great Plains. In some cases, cultivation of the Great Plains has increased the availability of water, causing dominant sulfate salts to travel from the uphill areas to depressions where it will surface when water evaporates at the soil surface. A potential solution could include the replanting of the native deep-rooted vegetation, which requires knowledge of the spatial distribution of soil salinity. This study tested the soil factors influencing electromagnetic-induction meter (EM38) readings of soil salinity distribution around wetlands. The objectives were to: (1) predict growth and survival of Salix dasyclados Wimm. (cv. `India') along a salinity gradient in a small wetland, and (2) investigate whether newly established willows affected water-table fluctuations, which would indicate their phreatophytic nature or their ability to obtain their water supply from the zone of saturation. Results indicated significantly lower salinity values for sampling points with EM38 readings above 175 and 250 mS m-1 for height and survival, respectively. In addition, diurnal fluxes of the water table in areas of good willow growth and lower salinity indicated that cultivar `India' was phreatophytic in these areas and therefore has great potential for being used to combat saline seeps.

Conductivity gradients as inferred by electromagnetic-induction meter (EM38) readings within a salt-affected wetland in Saskatchewan, Canada

NASA Astrophysics Data System (ADS)

Mirck, Jaconette; Schroeder, William

2018-06-01

The change from deep-rooted grass and shrub vegetation to annual-cropping dryland farming has contributed to serious soil salinization challenges on the semi-arid North American Great Plains. In some cases, cultivation of the Great Plains has increased the availability of water, causing dominant sulfate salts to travel from the uphill areas to depressions where it will surface when water evaporates at the soil surface. A potential solution could include the replanting of the native deep-rooted vegetation, which requires knowledge of the spatial distribution of soil salinity. This study tested the soil factors influencing electromagnetic-induction meter (EM38) readings of soil salinity distribution around wetlands. The objectives were to: (1) predict growth and survival of Salix dasyclados Wimm. (cv. `India') along a salinity gradient in a small wetland, and (2) investigate whether newly established willows affected water-table fluctuations, which would indicate their phreatophytic nature or their ability to obtain their water supply from the zone of saturation. Results indicated significantly lower salinity values for sampling points with EM38 readings above 175 and 250 mS m-1 for height and survival, respectively. In addition, diurnal fluxes of the water table in areas of good willow growth and lower salinity indicated that cultivar `India' was phreatophytic in these areas and therefore has great potential for being used to combat saline seeps.

KSC-04pd1287

NASA Image and Video Library

2004-06-10

KENNEDY SPACE CENTER, FLA. - Three wild pigs - a mother and her two offspring - root for food in the grass near the NASA News Center at KSC. Feral pigs were introduced to Florida in the 1500s and are now found statewide in wooded areas close to water. The pigs have flourished in the environs around KSC, which shares a border with the Merritt Island National Wildlife Refuge, without many predators other than panthers and humans. Pigs are omnivores, foraging on the ground and rooting just beneath the surface, which damages the groundcover. Wild pigs eat almost anything that has nutritional value, including tubers, roots, shoots, acorns, fruits, berries, earthworms, amphibians, reptiles and rodents. Appearance is similar to domestic hogs, but leaner, with a longer, narrower head and a coarser, denser coat. Females may have two litters per year. The piglets are weaned in a few weeks but remain with the mother for several months.

KSC-04pd1285

NASA Image and Video Library

2004-06-10

KENNEDY SPACE CENTER, FLA. - Three wild pigs - a mother and her two offspring - root for food in the grass near the NASA News Center at KSC. Feral pigs were introduced to Florida in the 1500s and are now found statewide in wooded areas close to water. The pigs have flourished in the environs around KSC, which shares a border with the Merritt Island National Wildlife Refuge, without many predators other than panthers and humans. Pigs are omnivores, foraging on the ground and rooting just beneath the surface, which damages the groundcover. Wild pigs eat almost anything that has nutritional value, including tubers, roots, shoots, acorns, fruits, berries, earthworms, amphibians, reptiles and rodents. Appearance is similar to domestic hogs, but leaner, with a longer, narrower head and a coarser, denser coat. Females may have two litters per year. The piglets are weaned in a few weeks but remain with the mother for several months.

KSC-2009-2107

NASA Image and Video Library

2009-03-03

CAPE CANAVERAL, Fla. – At NASA's Kennedy Space Center in Florida, a family of wild pigs roots through the grass for food. The wild pigs have flourished in the environs around Kennedy, which shares a border with the Merritt Island National Wildlife Refuge, without many predators other than panthers and humans. Pigs were introduced to Florida in the 1500s and are now found statewide in wooded areas close to water. Pigs are omnivores, foraging on the ground and rooting just beneath the surface, which damages the groundcover. Wild pigs eat almost anything that has nutritional value, including tubers, roots, shoots, acorns, fruits, berries, earthworms, amphibians, reptiles and rodents. Their appearance is similar to domestic hogs, but leaner, with a longer, narrower head and a coarser, denser coat. Females may have two litters per year. The piglets are weaned in a few weeks but remain with the mother for several months. Photo credit: NASA/Ben Smegelsky

KSC-2009-2106

NASA Image and Video Library

2009-03-03

CAPE CANAVERAL, Fla. – At NASA's Kennedy Space Center in Florida, a family of wild pigs roots through the grass for food. The wild pigs have flourished in the environs around Kennedy, which shares a border with the Merritt Island National Wildlife Refuge, without many predators other than panthers and humans. Pigs were introduced to Florida in the 1500s and are now found statewide in wooded areas close to water. Pigs are omnivores, foraging on the ground and rooting just beneath the surface, which damages the groundcover. Wild pigs eat almost anything that has nutritional value, including tubers, roots, shoots, acorns, fruits, berries, earthworms, amphibians, reptiles and rodents. Their appearance is similar to domestic hogs, but leaner, with a longer, narrower head and a coarser, denser coat. Females may have two litters per year. The piglets are weaned in a few weeks but remain with the mother for several months. Photo credit: NASA/Ben Smegelsky

KSC-04PD-1285

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. Three wild pigs a mother and her two offspring root for food in the grass near the NASA News Center at KSC. Feral pigs were introduced to Florida in the 1500s and are now found statewide in wooded areas close to water. The pigs have flourished in the environs around KSC, which shares a border with the Merritt Island National Wildlife Refuge, without many predators other than panthers and humans. Pigs are omnivores, foraging on the ground and rooting just beneath the surface, which damages the groundcover. Wild pigs eat almost anything that has nutritional value, including tubers, roots, shoots, acorns, fruits, berries, earthworms, amphibians, reptiles and rodents. Appearance is similar to domestic hogs, but leaner, with a longer, narrower head and a coarser, denser coat. Females may have two litters per year. The piglets are weaned in a few weeks but remain with the mother for several months.

KSC-04PD-1287

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. Three wild pigs a mother and her two offspring root for food in the grass near the NASA News Center at KSC. Feral pigs were introduced to Florida in the 1500s and are now found statewide in wooded areas close to water. The pigs have flourished in the environs around KSC, which shares a border with the Merritt Island National Wildlife Refuge, without many predators other than panthers and humans. Pigs are omnivores, foraging on the ground and rooting just beneath the surface, which damages the groundcover. Wild pigs eat almost anything that has nutritional value, including tubers, roots, shoots, acorns, fruits, berries, earthworms, amphibians, reptiles and rodents. Appearance is similar to domestic hogs, but leaner, with a longer, narrower head and a coarser, denser coat. Females may have two litters per year. The piglets are weaned in a few weeks but remain with the mother for several months.

A comparison of root surface temperatures using different obturation heat sources.

PubMed

Lee, F S; Van Cura, J E; BeGole, E

1998-09-01

This study compared root surface temperatures produced during warm vertical obturation using the System B Heat Source (SB), the Touch 'n Heat device (TH), and a flame-heated carrier (FH). The root canals of 30 maxillary incisor, premolar, and mandibular incisor teeth were prepared; divided into three groups; and obturated using each heat source. A thermocouple placed 2 mm below the cementoenamel junction transferred the temperature rise on the external root surface to a digital thermometer. SB surface temperature rise was < 10 degrees C for all experimental teeth. TH temperature rise in maxillary incisors and premolars was < 10 degrees C; however, > 10 degrees C was observed for mandibular incisors. FH produced a > 10 degrees C surface temperature rise in all experimental teeth. The critical level of root surface heat required to produce irreversible bone damage is believed to be > 10 degrees C. The findings of this study suggest that warm vertical condensation with the SB should not damage supporting periradicular tissues. However, caution should be used with TH and FH on mandibular incisors.

Cemental tear: To know what we have neglected in dental practice.

PubMed

Jeng, Po-Yuan; Luzi, Arlinda Luzi; Pitarch, Rocio Marco; Chang, Mei-Chi; Wu, Yu-Hsueh; Jeng, Jiiang-Huei

2018-04-01

Cemental tear is a special kind of root surface fracture, contributing to periodontal and periapical breakdown. However, it is a challenge for doctors to diagnose, resulting in delayed or improper treatment. We reviewed the predisposing factors, location, radiographic/clinical characteristics, diagnosis and treatments of cemental tears. From the literature, patients with cemental tear were mainly males, over 60 year-old. Possible predisposing factors include gender, age, tooth type, traumatic occlusal force and vital teeth. Cemental tears were common in upper and lower anterior teeth, single or multiple, and can be present in cervical, middle and apical third of roots. Morphology of cemental tears can be either piece-shaped or U-shaped. Clinically, cemental tear shows a unitary periodontal pocket and signs/symptoms mimicking localized periodontitis, apical periodontitis and vertical root fractures. Treatment of cemental tears include scaling, root planning, root canal treatment, periodontal/periapical surgery, guided tissue regeneration, bone grafting, and intentional replantation. Recurrence of cemental tear is possible especially when the fracture involves root apex. Extraction is recommended for teeth with poor prognosis. In conclusion, cemental tears can involve both periodontal and periapical area. Dentists should understand the predisposing factors and clinical features of cemental tears for early diagnosis/treatment to prevent bone loss/tooth extraction. Copyright © 2017. Published by Elsevier B.V.

[Effects of elevated ozone concentrations on enzyme activities and organic acids content in wheat rhizospheric soil.

PubMed

Yin, Wei Qin; Jing, Hao Qi; Wang, Ya Bo; Wei, Si Yu; Sun, Yue; Wang, Sheng Sen; Wang, Xuai Zhi

2018-02-01

The elevated concentration of tropospheric ozone (O 3 ) is an important global climate change driver, with adverse impacts on soil ecological environment and crop growth. In this study, a pot experiment was carried out in an open top chamber (OTC), to investigate the effects of elevated ozone concentration on soil enzyme activities (catalase, polyphenol oxidase, dehydrogenase and invertase), organic acids contents (oxalic acid, citric acid and malic acid) at different growth stages (tillering, jointing, heading and ripening stages) of wheat, and combined with the rhizospheric soil physicochemical properties and plant root characteristics to analyze the underlying reasons. The results showed that, elevated ozone concentration increased soil catalase, polyphenol oxidase, dehydrogenase and invertase activities at wheat ripening period to different degrees, with the effects on the activities of catalase and polyphenol oxidase being statistically significant. At the heading stage, activities of dehydrogenase and invertase were significantly increased by up to 76.7%. At the ripening stage, elevated ozone concentration significantly increased the content of citric acid and malic acid and redox potential (Eh) in rhizospheric soil, but reduced soil pH, electrical conductivity, total carbon and nitrogen. For root characteristics, elevated ozone concentrations significantly reduced the wheat root biomass, total root length and root surface area but increased the average root diameter.

Vacuum insulation of the high energy negative ion source for fusion application

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kojima, A.; Hanada, M.; Inoue, T.

2012-02-15

Vacuum insulation on a large size negative ion accelerator with multiple extraction apertures and acceleration grids for fusion application was experimentally examined and designed. In the experiment, vacuum insulation characteristics were investigated in the JT-60 negative ion source with >1000 apertures on the grid with the surface area of {approx}2 m{sup 2}. The sustainable voltages varied with a square root of the gap lengths between the grids, and decreased with number of the apertures and with the surface area of the grids. Based on the obtained results, the JT-60SA (super advanced) negative ion source is designed to produce 22 A,more » 500 keV D{sup -} ion beams for 100 s.« less

Effects of elevated CO2 levels on root morphological traits and Cd uptakes of two Lolium species under Cd stress*

PubMed Central

Jia, Yan; Tang, Shi-rong; Ju, Xue-hai; Shu, Li-na; Tu, Shu-xing; Feng, Ren-wei; Giusti, Lorenzino

2011-01-01

This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam. and Lolium perenne L. exposed to two CO2 levels (360 and 1000 μl/L) and three Cd levels (0, 4, and 16 mg/L) under hydroponic conditions. The results show that elevated levels of CO2 increased shoot biomass more, compared to root biomass, but decreased Cd concentrations in all plant tissues. Cd exposure caused toxicity to both Lolium species, as shown by the restrictions of the root morphological parameters including root length, surface area, volume, and tip numbers. These parameters were significantly higher under elevated levels of CO2 than under ambient CO2, especially for the number of fine roots. The increases in magnitudes of those parameters triggered by elevated levels of CO2 under Cd stress were more than those under non-Cd stress, suggesting an ameliorated Cd stress under elevated levels of CO2. The total Cd uptake per pot, calculated on the basis of biomass, was significantly greater under elevated levels of CO2 than under ambient CO2. Ameliorated Cd toxicity, decreased Cd concentration, and altered root morphological traits in both Lolium species under elevated levels of CO2 may have implications in food safety and phytoremediation. PMID:21462388

Root architecture and wind-firmness of mature Pinus pinaster.

PubMed

Danjon, Frédéric; Fourcaud, Thierry; Bert, Didier

2005-11-01

This study aims to link three-dimensional coarse root architecture to tree stability in mature timber trees with an average of 1-m rooting depth. Undamaged and uprooted trees were sampled in a stand damaged by a storm. Root architecture was measured by three-dimensional (3-D) digitizing. The distribution of root volume by root type and in wind-oriented sectors was analysed. Mature Pinus pinaster root systems were organized in a rigid 'cage' composed of a taproot, the zone of rapid taper of horizontal surface roots and numerous sinkers and deep roots, imprisoning a large mass of soil and guyed by long horizontal surface roots. Key compartments for stability exhibited strong selective leeward or windward reinforcement. Uprooted trees showed a lower cage volume, a larger proportion of oblique and intermediate depth horizontal roots and less wind-oriented root reinforcement. Pinus pinaster stability on moderately deep soils is optimized through a typical rooting pattern and a considerable structural adaptation to the prevailing wind and soil profile.

Potential for temperature change during application of ultrasonic vibration to intra-radicular posts.

PubMed

Satterthwaite, Julian D; Stokes, Alastair N; Frankel, Nicholas T N

2003-06-01

The aim of this study was to assess the potential for heat production when intra-radicular posts were subjected to ultrasonic vibration. Thirty zirconium ceramic posts and thirty stainless steel posts were luted into canine roots. Ultrasonic vibration was applied to the top of each post for thirty minutes and temperature change on the root surface was measured. The mean peak temperature rise from baseline was 18.7 degrees C. Post type had no influence on peak temperature. Temperature increase on the external root surfaces increased as the thickness of dentine between post and root surface reduced.

Effect of the association between citric acid and EDTA on root surface etching.

PubMed

Manzolli Leite, Fabio Renato; Nascimento, Gustavo Giacomelli; Manzolli Leite, Elza Regina; Leite, Amauri Antiquera; Cezar Sampaio, Josá Eduardo

2013-09-01

This study aims to compare the clot stabilization on root surfaces conditioned with citric acid and ethylenediamine-tetraacetic acid (EDTA). Scaled root samples (n = 100) were set in fve groups: group I-control group (saline solution); group II (24% EDTA); group III (25% citric acid); group IV (EDTA + citric acid); group V (citric acid + EDTA). Fifty samples were assessed using the root surface modifcation index (RSMI). The other 50 received a blood drop after conditioning. Clot formation was assessed using blood elements adhesion index (BEAI). A blind examiner evaluated photomicrographs. Statistical analysis considered p < 0.05. Groups-III and G-V attained the best results for RSMI and BEAI in comparison to control. The worst results for clot stabilization were seen in group-II. EDTA employment before citric acid (group-IV) reduced clot formation in comparison to citric acid use alone (group-III). Root conditioning with citric acid alone and before EDTA had the best results for smear layer removal and clot stabilization. EDTA inhibited clot stabilization on root surface and must have a residual activity once it has diminished clot adhesion to root even after citric acid conditioning. Thus, EDTA can be used to neutralize citric acid effects on periodontal cells without affecting clot stabilization. Clinical signifcance: To demonstrate that citric acid use on root surfaces previously affected by periodontal disease may favor clot stabilization and may have a benefcial effect on surgical outcomes. Also, EDTA can be used to neutralize citric acid effects on periodontal cells.

Waving and skewing: how gravity and the surface of growth media affect root development in Arabidopsis.

PubMed

Oliva, Michele; Dunand, Christophe

2007-01-01

Arabidopsis seedlings growing on inclined agar surfaces exhibit characteristic root behaviours called 'waving' and 'skewing': the former consists of a series of undulations, whereas the latter is a deviation from the direction of gravity. Even though the precise basis of these growth patterns is not well understood, both gravity and the contact between the medium and the root are considered to be the major players that result in these processes. The influence of these forces on root surface-dependent behaviours can be verified by growing seedlings at different gel pitches: plants growing on vertical plates present roots with slight waving and skewing when compared with seedlings grown on plates held at minor angles of < 90 degrees . However, other factors are thought to modulate root growth on agar; for instance, it has been demonstrated that the presence and concentration of certain compounds in the medium (such as sucrose) and of drugs able to modify the plant cell cytoskeleton also affect skewing and waving. The recent discovery of an active role of ethylene on surface-dependent root behaviour, and the finding of new mutants showing anomalous growth, pave the way for a more detailed description of these phenomena.

Cone-beam computed tomography analysis of curved root canals after mechanical preparation with three nickel-titanium rotary instruments

PubMed Central

Elsherief, Samia M.; Zayet, Mohamed K.; Hamouda, Ibrahim M.

2013-01-01

Cone beam computed tomography is a 3-dimensional high resolution imaging method. The purpose of this study was to compare the effects of 3 different NiTi rotary instruments used to prepare curved root canals on the final shape of the curved canals and total amount of root canal transportation by using cone-beam computed tomography. A total of 81 mesial root canals from 42 extracted human mandibular molars, with a curvature ranging from 15 to 45 degrees, were selected. Canals were randomly divided into 3 groups of 27 each. After preparation with Protaper, Revo-S and Hero Shaper, the amount of transportation and centering ability that occurred were assessed by using cone beam computed tomography. Utilizing pre- and post-instrumentation radiographs, straightening of the canal curvatures was determined with a computer image analysis program. Canals were metrically assessed for changes (surface area, changes in curvature and transportation) during canal preparation by using software SimPlant; instrument failures were also recorded. Mean total widths and outer and inner width measurements were determined on each central canal path and differences were statistically analyzed. The results showed that all instruments maintained the original canal curvature well with no significant differences between the different files (P = 0.226). During preparation there was failure of only one file (the protaper group). In conclusion, under the conditions of this study, all instruments maintained the original canal curvature well and were safe to use. Areas of uninstrumented root canal wall were left in all regions using the various systems. PMID:23885273

Adsorption of Eu(III) onto roots of water hyacinth

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kelley, C.; Mielke, R.E.; Dimaquibo, D.

1999-05-01

The water hyacinth (Eichhornia crassipes) has drawn attention as a plant capable of removing pollutants, including toxic metals, from water. The authors are interested in the capacity of the water hyacinth to remediate aquatic environments that have been contaminated with the lanthanide metal, europium Eu(III). Using scanning electron microscopy (SEM) they have been able to determine that Eu(III) is adsorbed onto the surface of the roots from water and that the highest concentration of Eu(III) is on the root hairs. X-ray absorption spectroscopy (XAS) techniques were used to speciate the Eu(III) adsorbed onto the surface of the roots. The XASmore » data for Eu-contaminated water hyacinth roots provides evidence of a Eu-oxygen environment and establishes that Eu(III) is coordinated to 10--11 oxygen atoms at a distance of 2.44 {angstrom}. This likely involves binding of Eu(III) to the root via carboxylate groups and hydration of Eu(III) at the root surface.« less

Multiwall carbon nanotubes modulate paraquat toxicity in Arabidopsis thaliana.

PubMed

Fan, Xiaoji; Xu, Jiahui; Lavoie, Michel; Peijnenburg, W J G M; Zhu, Youchao; Lu, Tao; Fu, Zhengwei; Zhu, Tingheng; Qian, Haifeng

2018-02-01

Carbon nanotubes can be either toxic or beneficial to plant growth and can also modulate toxicity of organic contaminants through surface sorption. The complex interacting toxic effects of carbon nanotubes and organic contaminants in plants have received little attention in the literature to date. In this study, the toxicity of multiwall carbon nanotubes (MWCNT, 50 mg/L) and paraquat (MV, 0.82 mg/L), separately or in combination, were evaluated at the physiological and the proteomic level in Arabidopsis thaliana for 7-14 days. The results revealed that the exposure to MWCNT had no inhibitory effect on the growth of shoots and leaves. Rather, MWCNT stimulated the relative electron transport rate and the effective photochemical quantum yield of PSII value as compared to the control by around 12% and lateral root production up to nearly 4-fold as compared to the control. The protective effect of MWCNT on MV toxicity on the root surface area could be quantitatively explained by the extent of MV adsorption on MWCNT and was related to stimulation of photosynthesis, antioxidant protection and number and area of lateral roots which in turn helped nutrient assimilation. The influence of MWCNT and MV on photosynthesis and oxidative stress at the physiological level was consistent with the proteomics analysis, with various over-expressed photosynthesis-related proteins (by more than 2 folds) and various under-expressed oxidative stress related proteins (by about 2-3 folds). This study brings new insights into the interactive effects of two xenobiotics (MWCNT and MV) on the physiology of a model plant. Copyright © 2017 Elsevier Ltd. All rights reserved.

The attachment of V79 and human periodontal ligament fibroblasts on periodontally involved root surfaces following treatment with EDTA, citric acid, or tetracycline HCL: an SEM in vitro study.

PubMed

Chandra, R Viswa; Jagetia, Ganesh Chandra; Bhat, K Mahalinga

2006-02-15

The present in vitro study has been designed to establish and compare the effects of citric acid, EDTA, and tetracycline HCl on human periodontally diseased roots on the structure, attachment, and orientation of V79 (primary Chinese hamster lung fibroblasts) cells and human periodontal ligament fibroblasts (HPDL). Commercially available V79 cells and HPDL derived from healthy human third molars were used in this study. These fibroblasts were left in solution for seven days in order to attain confluence. Forty single-rooted teeth were obtained from patients diagnosed with periodontitis. The crown part was removed under constant irrigation and the root was split vertically into two equal halves, thus, yielding 80 specimens. Following scaling and root planing, the specimens were washed with phosphate buffered saline (PBS) and kept in 50 microg/ml gentamycin sulphate solution for 24 hours. The root pieces were then treated as follows: citric acid at pH 1, 24% EDTA, or with a 10% solution of tetracycline HCl and were then placed in V79 fibroblast cultures and HPDL cultures. The specimens were harvested after four weeks and were fixed in 2.5% glutaraldehyde in PBS before preparation for scanning electron microscopy (SEM). The behavior of V79 cells was similar to that of human periodontal ligament cells on root conditioned surfaces. V79 and HPDL showed a healthy morphology on root surfaces treated with citric acid and EDTA and a relatively unhealthy appearance on root surfaces treated with tetracycline HCl and distilled water (control group). The results suggest the use of citric acid and EDTA as root conditioning agents favorably affects the migration, attachment, and morphology of fibroblasts on human root surfaces, which may play a significant role in periodontal healing and regeneration.

Aligned hierarchical Ag/ZnO nano-heterostructure arrays via electrohydrodynamic nanowire template for enhanced gas-sensing properties.

PubMed

Yin, Zhouping; Wang, Xiaomei; Sun, Fazhe; Tong, Xiaohu; Zhu, Chen; Lv, Qiying; Ye, Dong; Wang, Shuai; Luo, Wei; Huang, YongAn

2017-09-22

Gas sensing performance can be improved significantly by the increase in both the effective gas exposure area and the surface reactivitiy of ZnO nanorods. Here, we propose aligned hierarchical Ag/ZnO nano-heterostructure arrays (h-Ag/ZnO-NAs) via electrohydrodynamic nanowire template, together with a subsequent hydrothermal synthesis and photoreduction reaction. The h-Ag/ZnO-NAs scatter at top for higher specific surface areas with the air, simultaneously contact at root for the electrical conduction. Besides, the ZnO nanorods are uniformly coated with dispersed Ag nanoparticles, resulting in a tremendous enhancement of the surface reactivity. Compared with pure ZnO, such h-Ag/ZnO-NAs exhibit lower electrical resistance and faster responses. Moreover, they demonstrate enhanced NO 2 gas sensing properties. Self-assembly via electrohydrodynamic nanowire template paves a new way for the preparation of high performance gas sensors.

Crustal structure and gravity anomalies beneath the Rif, northern Morocco: implications for the current tectonics of the Alboran region

NASA Astrophysics Data System (ADS)

Petit, Carole; Le Pourhiet, Laetitia; Scalabrino, Bruno; Corsini, Michel; Bonnin, Mickaël; Romagny, Adrien

2015-07-01

We analyse Bouguer anomaly data and previously published Moho depths estimated from receiver functions in order to determine the amount of isostatic compensation or uncompensation of the Rif topography in northern Morocco. We use Moho depth variations extracted from receiver function analyses to predict synthetic Bouguer anomalies that are then compared to observed Bouguer anomaly. We find that Moho depth variations due to isostatic compensation of topographic and/or intracrustal loads do not match Moho depth estimates obtained from receiver function analyses. The isostatic misfit map evidences excess crustal root as large as 10 km in the western part of the study area, whereas a `missing' crustal root of ˜5 km appears east of 4.3°E. This excess root/missing topography correlates with the presence of a dense mantle lid, the noticeable southwestward drift of the Western Rif area, and with a current surface uplift. We propose that a delaminated mantle lid progressively detaching westward or southwestward from the overlying crust is responsible for viscous flow of the ductile lower crust beneath the Rif area. This gives rise to isostatic uplift and westward drift due to viscous coupling at the upper/lower crust boundary. At the same time, the presence of this dense sinking mantle lid causes a negative dynamic topography, which explains why the observed topography is too low compared to the crustal thickness.

Uptake of uranium by aquatic plants growing in fresh water ecosystem around uranium mill tailings pond at Jaduguda, India.

PubMed

Jha, V N; Tripathi, R M; Sethy, N K; Sahoo, S K

2016-01-01

Concentration of uranium was determined in aquatic plants and substrate (sediment or water) of fresh water ecosystem on and around uranium mill tailings pond at Jaduguda, India. Aquatic plant/substrate concentration ratios (CRs) of uranium were estimated for different sites on and around the uranium mill tailings disposal area. These sites include upstream and downstream side of surface water sources carrying the treated tailings effluent, a small pond inside tailings disposal area and residual water of this area. Three types of plant groups were investigated namely algae (filamentous and non-filamentous), other free floating & water submerged and sediment rooted plants. Wide variability in concentration ratio was observed for different groups of plants studied. The filamentous algae uranium concentration was significantly correlated with that of water (r=0.86, p<0.003). For sediment rooted plants significant correlation was found between uranium concentration in plant and the substrate (r=0.88, p<0.001). Both for other free floating species and sediment rooted plants, uranium concentration was significantly correlated with Mn, Fe, and Ni concentration of plants (p<0.01). Filamentous algae, Jussiaea and Pistia owing to their high bioproductivity, biomass, uranium accumulation and concentration ratio can be useful for prospecting phytoremediation of stream carrying treated or untreated uranium mill tailings effluent. Copyright © 2015 Elsevier B.V. All rights reserved.

Root canal preparation with a novel nickel-titanium instrument evaluated with micro-computed tomography: canal surface preparation over time.

PubMed

Peters, Ove A; Boessler, Claudia; Paqué, Frank

2010-06-01

The aim of this part of an ongoing study was to describe the dentin removal ability of a novel nickel-titanium instrument, the self-adjusting file (SAF), by using micro-computed tomography. Twenty maxillary incisors were scanned preoperatively at 20-microm resolution and postoperatively after up to 6 minutes of preparation with an SAF with 1.5-mm or 2-mm diameter. SAFs were operated with continuous irrigation in a handpiece that provided an oscillating, in-and-out movement. Changes in canal volume compared with preoperative values as well as unprepared canal surface area were determined. Data were normally distributed and compared by analysis of variance and regression analyses. Preoperatively canal volumes were statistically similar in both groups (9.86 +/- 3.97 mm3 and 9.80 +/- 2.67 mm3). Volumes increased during preparation to 13.58 +/- 3.85 mm3 (after 6 minutes with SAF 1.5 mm) and 16.43 +/- 3.64 mm3 (after 5 minutes with SAF 2.0 mm), and overall canal shapes were adequate. Unprepared canal surface area decreased from 63.0% +/- 15.1% (2 minutes with SAF 1.5 mm) to 8.6% +/- 4.1% (5 minutes with SAF 2.0 mm). Preparation of straight root canals in maxillary anterior teeth left little canal surface uninstrumented after shaping with the SAF. The timeframe of clinical application will depend on the amount of desired dentin removal and done with an SAF selected on the basis of apical gauging. Copyright 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Differences in Fine-Root Biomass of Trees and Understory Vegetation among Stand Types in Subtropical Forests

PubMed Central

Fu, Xiaoli; Wang, Jianlei; Di, Yuebao; Wang, Huimin

2015-01-01

Variation of total fine-root biomass among types of tree stands has previously been attributed to the characteristics of the stand layers. The effects of the understory vegetation on total fine-root biomass are less well studied. We examined the variation of total fine-root biomass in subtropical tree stands at two sites of Datian and Huitong in China. The two sites have similar humid monsoon climate but different soil organic carbon. One examination compared two categories of basal areas (high vs. low basal area) in stands of single species. A second examination compared single-species and mixed stands with comparable basal areas. Low basal area did not correlate with low total fine-root biomass in the single-species stands. The increase in seedling density but decrease in stem density for the low basal area stands at Datian and the quite similar stand structures for the basal-area contrast at Huitong helped in the lack of association between basal area and total fine-root biomass at the two sites, respectively. The mixed stands also did not yield higher total fine-root biomasses. In addition to the lack of niche complementarity between tree species, the differences in stem and seedling densities and the belowground competition between the tree and non-tree species also contributed to the similarity of the total fine-root biomasses in the mixed and single-species stands. Across stand types, the more fertile site Datian yielded higher tree, non-tree and total fine-root biomasses than Huitong. However, the contribution of non-tree fine-root biomass to the total fine-root biomass was higher at Huitong (29.4%) than that at Datian (16.7%). This study suggests that the variation of total fine-root biomass across stand types not only was associated with the characteristics of trees, but also may be highly dependent on the understory layer. PMID:26047358

Differences in Fine-Root Biomass of Trees and Understory Vegetation among Stand Types in Subtropical Forests.

PubMed

Fu, Xiaoli; Wang, Jianlei; Di, Yuebao; Wang, Huimin

2015-01-01

Variation of total fine-root biomass among types of tree stands has previously been attributed to the characteristics of the stand layers. The effects of the understory vegetation on total fine-root biomass are less well studied. We examined the variation of total fine-root biomass in subtropical tree stands at two sites of Datian and Huitong in China. The two sites have similar humid monsoon climate but different soil organic carbon. One examination compared two categories of basal areas (high vs. low basal area) in stands of single species. A second examination compared single-species and mixed stands with comparable basal areas. Low basal area did not correlate with low total fine-root biomass in the single-species stands. The increase in seedling density but decrease in stem density for the low basal area stands at Datian and the quite similar stand structures for the basal-area contrast at Huitong helped in the lack of association between basal area and total fine-root biomass at the two sites, respectively. The mixed stands also did not yield higher total fine-root biomasses. In addition to the lack of niche complementarity between tree species, the differences in stem and seedling densities and the belowground competition between the tree and non-tree species also contributed to the similarity of the total fine-root biomasses in the mixed and single-species stands. Across stand types, the more fertile site Datian yielded higher tree, non-tree and total fine-root biomasses than Huitong. However, the contribution of non-tree fine-root biomass to the total fine-root biomass was higher at Huitong (29.4%) than that at Datian (16.7%). This study suggests that the variation of total fine-root biomass across stand types not only was associated with the characteristics of trees, but also may be highly dependent on the understory layer.

Water flow and solute transport in floating fen root mats

NASA Astrophysics Data System (ADS)

Stofberg, Sija F.; EATM van der Zee, Sjoerd

2015-04-01

Floating fens are valuable wetlands, found in North-Western Europe, that are formed by floating root mats when old turf ponds are colonized by plants. These terrestrialization ecosystems are known for their biodiversity and the presence of rare plant species, and the root mats reveal different vegetation zones at a small scale. The vegetation zones are a result of strong gradients in abiotic conditions, including groundwater dynamics, nutrients and pH. To prevent irreversible drought effects such as land subsidence and mineralization of peat, water management involves import of water from elsewhere to maintain constant surface water levels. Imported water may have elevated levels of salinity during dry summers, and salt exposure may threaten the vegetation. To assess the risk of exposure of the rare plant species to salinity, the hydrology of such root mats must be understood. Physical properties of root mats have scarcely been investigated. We have measured soil characteristics, hydraulic conductivity, vertical root mat movement and groundwater dynamics in a floating root mat in the nature reserve Nieuwkoopse Plassen, in the Netherlands. The root mat mostly consists of roots and organic material, in which the soil has a high saturated water content, and strongly varies in its stage of decomposition. We have found a distinct negative correlation between degree of decomposition and hydraulic conductivity, similar to observations for bogs in the literature. Our results show that the relatively young, thin edge of the root mat that colonizes the surface water has a high hydraulic conductivity and floats in the surface water, resulting in very small groundwater fluctuations within the root mat. The older part of the root mat, that is connected to the deeper peat layers is hydrologically more isolated and the material has a lower conductivity. Here, the groundwater fluctuates strongly with atmospheric forcing. The zones of hydraulic properties and vegetation, appear to be very similar and likely functionally related. Our experimental field data were used for modelling water flow and solute transport in floating fens, using HYDRUS 2D. Fluctuations of surface water and root mat, as well as geometry and unsaturated zone parameters can have a major influence on groundwater fluctuations and the exchange between rain and surface water and the water in the root mats. In combination with the duration of salt pulses in surface water, and sensitivity of fen plants to salinity (Stofberg et al. 2014, submitted), risks for rare plants can be anticipated.

Isolation of a novel mutant gene for soil-surface rooting in rice (Oryza sativa L.)

PubMed Central

2013-01-01

Background Root system architecture is an important trait affecting the uptake of nutrients and water by crops. Shallower root systems preferentially take up nutrients from the topsoil and help avoid unfavorable environments in deeper soil layers. We have found a soil-surface rooting mutant from an M2 population that was regenerated from seed calli of a japonica rice cultivar, Nipponbare. In this study, we examined the genetic and physiological characteristics of this mutant. Results The primary roots of the mutant showed no gravitropic response from the seedling stage on, whereas the gravitropic response of the shoots was normal. Segregation analyses by using an F2 population derived from a cross between the soil-surface rooting mutant and wild-type Nipponbare indicated that the trait was controlled by a single recessive gene, designated as sor1. Fine mapping by using an F2 population derived from a cross between the mutant and an indica rice cultivar, Kasalath, revealed that sor1 was located within a 136-kb region between the simple sequence repeat markers RM16254 and 2935-6 on the terminal region of the short arm of chromosome 4, where 13 putative open reading frames (ORFs) were found. We sequenced these ORFs and detected a 33-bp deletion in one of them, Os04g0101800. Transgenic plants of the mutant transformed with the genomic fragment carrying the Os04g0101800 sequence from Nipponbare showed normal gravitropic responses and no soil-surface rooting. Conclusion These results suggest that sor1, a rice mutant causing soil-surface rooting and altered root gravitropic response, is allelic to Os04g0101800, and that a 33-bp deletion in the coding region of this gene causes the mutant phenotypes. PMID:24280269

Isolation of a novel mutant gene for soil-surface rooting in rice (Oryza sativa L.).

PubMed

Hanzawa, Eiko; Sasaki, Kazuhiro; Nagai, Shinsei; Obara, Mitsuhiro; Fukuta, Yoshimichi; Uga, Yusaku; Miyao, Akio; Hirochika, Hirohiko; Higashitani, Atsushi; Maekawa, Masahiko; Sato, Tadashi

2013-11-20

Root system architecture is an important trait affecting the uptake of nutrients and water by crops. Shallower root systems preferentially take up nutrients from the topsoil and help avoid unfavorable environments in deeper soil layers. We have found a soil-surface rooting mutant from an M2 population that was regenerated from seed calli of a japonica rice cultivar, Nipponbare. In this study, we examined the genetic and physiological characteristics of this mutant. The primary roots of the mutant showed no gravitropic response from the seedling stage on, whereas the gravitropic response of the shoots was normal. Segregation analyses by using an F2 population derived from a cross between the soil-surface rooting mutant and wild-type Nipponbare indicated that the trait was controlled by a single recessive gene, designated as sor1. Fine mapping by using an F2 population derived from a cross between the mutant and an indica rice cultivar, Kasalath, revealed that sor1 was located within a 136-kb region between the simple sequence repeat markers RM16254 and 2935-6 on the terminal region of the short arm of chromosome 4, where 13 putative open reading frames (ORFs) were found. We sequenced these ORFs and detected a 33-bp deletion in one of them, Os04g0101800. Transgenic plants of the mutant transformed with the genomic fragment carrying the Os04g0101800 sequence from Nipponbare showed normal gravitropic responses and no soil-surface rooting. These results suggest that sor1, a rice mutant causing soil-surface rooting and altered root gravitropic response, is allelic to Os04g0101800, and that a 33-bp deletion in the coding region of this gene causes the mutant phenotypes.

Evaluation of Root Canal Preparation Using Rotary System and Hand Instruments Assessed by Micro-Computed Tomography

PubMed Central

Stavileci, Miranda; Hoxha, Veton; Görduysus, Ömer; Tatar, Ilkan; Laperre, Kjell; Hostens, Jeroen; Küçükkaya, Selen; Muhaxheri, Edmond

2015-01-01

Background Complete mechanical preparation of the root canal system is rarely achieved. Therefore, the purpose of this study was to evaluate and compare the root canal shaping efficacy of ProTaper rotary files and standard stainless steel K-files using micro-computed tomography. Material/Methods Sixty extracted upper second premolars were selected and divided into 2 groups of 30 teeth each. Before preparation, all samples were scanned by micro-computed tomography. Thirty teeth were prepared with the ProTaper system and the other 30 with stainless steel files. After preparation, the untouched surface and root canal straightening were evaluated with micro-computed tomography. The percentage of untouched root canal surface was calculated in the coronal, middle, and apical parts of the canal. We also calculated straightening of the canal after root canal preparation. Results from the 2 groups were statistically compared using the Minitab statistical package. Results ProTaper rotary files left less untouched root canal surface compared with manual preparation in coronal, middle, and apical sector (p<0.001). Similarly, there was a statistically significant difference in root canal straightening after preparation between the techniques (p<0.001). Conclusions Neither manual nor rotary techniques completely prepared the root canal, and both techniques caused slight straightening of the root canal. PMID:26092929

Evaluation of Root Canal Preparation Using Rotary System and Hand Instruments Assessed by Micro-Computed Tomography.

PubMed

Stavileci, Miranda; Hoxha, Veton; Görduysus, Ömer; Tatar, Ilkan; Laperre, Kjell; Hostens, Jeroen; Küçükkaya, Selen; Muhaxheri, Edmond

2015-06-20

Complete mechanical preparation of the root canal system is rarely achieved. Therefore, the purpose of this study was to evaluate and compare the root canal shaping efficacy of ProTaper rotary files and standard stainless steel K-files using micro-computed tomography. Sixty extracted upper second premolars were selected and divided into 2 groups of 30 teeth each. Before preparation, all samples were scanned by micro-computed tomography. Thirty teeth were prepared with the ProTaper system and the other 30 with stainless steel files. After preparation, the untouched surface and root canal straightening were evaluated with micro-computed tomography. The percentage of untouched root canal surface was calculated in the coronal, middle, and apical parts of the canal. We also calculated straightening of the canal after root canal preparation. Results from the 2 groups were statistically compared using the Minitab statistical package. ProTaper rotary files left less untouched root canal surface compared with manual preparation in coronal, middle, and apical sector (p<0.001). Similarly, there was a statistically significant difference in root canal straightening after preparation between the techniques (p<0.001). Neither manual nor rotary techniques completely prepared the root canal, and both techniques caused slight straightening of the root canal.

Environmental and physiological effects on grouping of drought-tolerant and susceptible rice varieties related to rice (Oryza sativa) root hydraulics under drought

PubMed Central

Henry, Amelia; Wehler, Regina; Grondin, Alexandre; Franke, Rochus; Quintana, Marinell

2016-01-01

Background and Aims Root hydraulic limitations (i.e. intra-plant restrictions to water movement) may be related to crop performance under drought, and groupings in the hydraulic function of drought-tolerant and drought-susceptible rice (Oryza sativa) varieties have been previously reported. This study aimed to better understand the environmental and physiological relationships with rice root hydraulics under drought. Methods Xylem sap bleeding rates in the field (gsap g–1 shoot) were measured on seasonal and diurnal time frames, during which time environmental conditions were monitored and physiological measurements were conducted. Complementary experiments on the effects of vapour pressure deficit (VPD) on root hydraulic conductivity and on transpiration rates of de-rooted tillers were conducted in growth chambers. Key Results The diurnal effects on bleeding rate were more closely related to irradiance than VPD, and VPD effects on root hydraulic conductivity measured on 21-day-old plants were due to effects on plant growth including root surface area, maximum root depth and root:shoot ratio. Leaf osmotic potential was related to the grouping of drought-tolerant and drought-susceptible varieties in rice root hydraulics, and these groupings were independent of differences in phenology. Low single-tiller bleeding rates were observed under high evapo-transpirational demand, higher bleeding rates were observed at more negative leaf osmotic potentials in drought-susceptible varieties, and drought-tolerant and susceptible varieties differed in the VPD-induced increase in transpiration rates of de-rooted tillers. Low root suberin amounts in some of the drought-susceptible varieties may have resulted in higher ion transport, as evidenced by higher sap K+ concentration and higher bleeding rates in those varieties. Conclusions These results provide evidence of the environmental effects on shoots that can influence root hydraulics. The consistent groupings of drought-tolerant and susceptible varieties suggest that traits affecting plant osmotic status may regulate root hydraulic response to drought in rice. PMID:27192712

Electro and Magneto-Electropolished Surface Micro-Patterning on Binary and Ternary Nitinol

PubMed Central

Munroe, Norman; McGoron, Anthony

2012-01-01

In this study, an Atomic Force Microscopy (AFM) roughness analysis was performed on non-commercial Nitinol alloys with Electropolished (EP) and Magneto-Electropolished (MEP) surface treatments and commercially available stents by measuring Root-Mean-Square (RMS), Average Roughness (Ra), and Surface Area (SA) values at various dimensional areas on the alloy surfaces, ranging from (800 × 800 nm) to (115 × 115μm), and (800 × 800 nm) to (40 × 40 μm) on the commercial stents. Results showed that NiTi-Ta 10 wt% with an EP surface treatment yielded the highest overall roughness, while the NiTi-Cu 10 wt% alloy had the lowest roughness when analyzed over (115 × 115 μm). Scanning Electron Microscopy (SEM) and Energy Dispersive Spectroscopy (EDS) analysis revealed unique surface morphologies for surface treated alloys, as well as an aggregation of ternary elements Cr and Cu at grain boundaries in MEP and EP surface treated alloys, and non-surface treated alloys. Such surface micro-patterning on ternary Nitinol alloys could increase cellular adhesion and accelerate surface endothelialization of endovascular stents, thus reducing the likelihood of in-stent restenosis and provide insight into hemodynamic flow regimes and the corrosion behavior of an implantable device influenced from such surface micro-patterns. PMID:22754200

Effect of root planing on surface topography: an in-vivo randomized experimental trial.

PubMed

Rosales-Leal, J I; Flores, A B; Contreras, T; Bravo, M; Cabrerizo-Vílchez, M A; Mesa, F

2015-04-01

The root surface topography exerts a major influence on clinical attachment and bacterial recolonization after root planing. In-vitro topographic studies have yielded variable results, and clinical studies are necessary to compare root surface topography after planing with current ultrasonic devices and with traditional manual instrumentation. The aim of this study was to compare the topography of untreated single-rooted teeth planed in vivo with a curette, a piezoelectric ultrasonic (PU) scraper or a vertically oscillating ultrasonic (VOU) scraper. In a randomized experimental trial of 19 patients, 44 single-rooted teeth were randomly assigned to one of four groups for: no treatment; manual root planing with a curette; root planing with a PU scraper; or root planing with a VOU scraper. Post-treatment, the teeth were extracted and their topography was analyzed in 124 observations with white-light confocal microscopy, measuring the roughness parameters arithmetic average height, root-mean-square roughness, maximum height of peaks, maximum depth of valleys, absolute height, skewness and kurtosis. The roughness values arithmetic average height and root-mean-square roughness were similar after each treatment and lower than after no treatment ( p < 0.05). Absolute height was lower in the VOU group than in the untreated ( p = 0.0026) and PU (p = 0.045) groups. Surface morphology was similar after the three treatments and was less irregular than in the untreated group. Values for the remaining roughness parameters were similar among all treatment groups ( p > 0.05). Both ultrasonic devices reduce the roughness, producing a similar topography to that observed after manual instrumentation with a curette, to which they appear to represent a valid alternative. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

In vitro radicular temperatures produced by injectable thermoplasticized gutta-percha.

PubMed

Weller, R N; Koch, K A

1995-03-01

In vitro temperatures produced in the root canal and on the root surface were measured simultaneously as heated gutta-percha was injected into the prepared canal. The canals were obturated with the Obtura II heated gutta-percha system with temperature settings of 160, 185, and 200 degrees C. The mean intracanal temperatures ranged from 40.21 to 57.24 degrees C, whereas the mean root surface temperatures were recorded from 37.22 to 41.90 degrees C for all three temperatures tested. The rise in temperature on the root surface was below the critical level of 10 degrees C and should not cause damage to the periodontal ligament.

Adaptive fine root foraging patterns in climate experiments and natural gradients

NASA Astrophysics Data System (ADS)

Ostonen, Ivika; Truu, Marika; Parts, Kaarin; Truu, Jaak

2017-04-01

Site based manipulative experiments and studies along climatic gradients have long been keystones of ecological research. We aimed to compare the response of ectomycorrhizal (EcM) and fine roots in manipulative studies and along climate gradient to describe the universal trends in root traits and to raise hypotheses about general mechanisms in fine root system adaptation of forest trees in global change. The root traits from two climate manipulation experiments - Bangor FACE and FAHM in Estonia, manipulated by CO2 concentration and relative air humidity in silver birch forest ecosystems, respectively and the data for three most ubiquitous tree species - Norway spruce (Picea abies), Scots pine (Pinus sylvestris) and silver birch (Betula pendula) stands along natural gradient encompassing different climate and forest zones in Northern Europe were analysed. There are two main strategies in response of fine root system of trees: A) an extensive increase in absorptive root biomass, surface area and length, or B) a greater reliance on root-associated EcM fungi and bacterial communities with a smaller investment to absorptive root biomass. Trees in all studies tended to increase the EcM root biomass and the proportion of EcM root biomass of total fine root biomass towards harsh (northern boreal forests) or changed conditions (stress created by the increase in CO2 concentration or relative air humidity). We envisage a role of trilateral relation between the morphological traits of absorptive fine roots, exploration types of colonising EcM fungi and rhizosphere and bulk soil bacterial community structure. A significant change in EcM absorptive fine root biomass in all experiments and for all studied tree species coincided with changes in absorptive root morphology, being longer and thinner root tips with higher root tissue density in poor/treated sites. These changes were associated with significant shifts in community structure of dominating EcM fungi as well as soil and rhizosphere bacterial communities. We suggest a multidimensional concept of absorptive fine root foraging strategies involving both qualitative and quantitative changes in root-mycorhizosphere along environmental gradients and in climate experiments.

D-Area Drip Irrigation-Phytoremediation Project: SRTC Final Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilde, E.W.

2003-01-14

Groundwater in D-Area at the Savannah River Site (SRS) is contaminated with trichloroethylene (TCE) and by-products resulting from discharges of this organic solvent during past operations. Several potential clean-up strategies are being or have been investigated, including a novel drip irrigation-phytoremediation process that is the focus of the treatability study described in this report. The contaminated groundwater in D-Area occurs primarily at depths of 30 to 50 feet below ground surface, well below the depths that are typically penetrated by plant roots. The system investigated in this study involved pumping water from the contaminated aquifer and discharging the water intomore » overlying test plots below the surface using drip irrigation. The test plots contained pines, cottonwoods, or no vegetation (controls). The primary objective was to determine the overall effectiveness of the process for TCE removal and to elucidate the biotic and abiotic pathways for its removal.« less

Thermal and microstructural effects of nanosecond pulsed Nd:YAG laser irradiation on tooth root surface

NASA Astrophysics Data System (ADS)

Wilder-Smith, Petra B. B.; Arrastia-Jitosho, Anna-Marie A.; Grill, G.; Liaw, Lih-Huei L.; Berns, Michael W.

1995-05-01

Plaque, calculus and altered cementum removal by scaling and root planing is a fundamental procedure in periodontal treatment. However, the residual smear layer contains cytotoxic and inflammatory mediators which adversely affect healing. Chemical smear layer removal is also problematic. In previous investigations effective smear layer removal was achieved using long pulsed irradiation at 1.06 (mu) . However, laser irradiation was not adequate as an alternative to scaling and root planing procedures and concurrent temperature rises exceeded thermal thresholds for pulpal and periodontal safety. It was the aim of this study to determine whether nanosecond pulsed irradiation at 1.06 (mu) could be used as an alternative or an adjunct to scaling and root planing. Sixty freshly extracted teeth were divided as follows: 5 control, 5 root planed only, 25 irradiated only, 25 root planed and irradiated. Irradiation was performed at fluences of 0.5 - 2.7 J/cm2, total energy densities of 12 - 300 J/cm2, frequencies of 2 - 10 Hz using the Medlite (Continuum) laser. Irradiation-induced thermal events were recorded using a thermocouple within the root canal and a thermal camera to monitor surface temperatures. SEM demonstrated effective smear layer removal with minimal microstructural effects. Surface temperatures increased minimally (< 3 C) at all parameters, intrapulpal temperature rises remained below 4 C at 2 and 5 Hz, F < 0.5 J/cm2. Without prior scaling and root planing, laser effects did not provide an adequately clean root surface.

Echelon approach to areas of concern in synoptic regional monitoring

USGS Publications Warehouse

Myers, Wayne; Patil, Ganapati P.; Joly, Kyle

1997-01-01

Echelons provide an objective approach to prospecting for areas of potential concern in synoptic regional monitoring of a surface variable. Echelons can be regarded informally as stacked hill forms. The strategy is to identify regions of the surface which are elevated relative to surroundings (Relative ELEVATIONS or RELEVATIONS). These are areas which would continue to expand as islands with receding (virtual) floodwaters. Levels where islands would merge are critical elevations which delimit echelons in the vertical dimension. Families of echelons consist of surface sectors constituting separate islands for deeper waters that merge as water level declines. Pits which would hold water are disregarded in such a progression, but a complementary analysis of pits is obtained using the surface as a virtual mould to cast a counter-surface (bathymetric analysis). An echelon tree is a family tree of echelons with peaks as terminals and the lowest level as root. An echelon tree thus provides a dendrogram representation of surface topology which enables graph theoretic analysis and comparison of surface structures. Echelon top view maps show echelon cover sectors on the base plane. An echelon table summarizes characteristics of echelons as instances or cases of hill form surface structure. Determination of echelons requires only ordinal strength for the surface variable, and is thus appropriate for environmental indices as well as measurements. Since echelons are inherent in a surface rather than perceptual, they provide a basis for computer-intelligent understanding of surfaces. Echelons are given for broad-scale mammalian species richness in Pennsylvania.

Root gravitropism in maize and Arabidopsis

NASA Technical Reports Server (NTRS)

Evans, Michael L.

1993-01-01

Research during the period 1 March 1992 to 30 November 1993 focused on improvements in a video digitizer system designed to automate the recording of surface extension in plants responding to gravistimulation. The improvements included modification of software to allow detailed analysis of localized extension patterns in roots of Arabidopsis. We used the system to analyze the role of the postmitotic isodiametric growth zone (a region between the meristem and the elongation zone) in the response of maize roots to auxin, calcium, touch and gravity. We also used the system to analyze short-term auxin and gravitropic responses in mutants of Arabidopsis with reduced auxin sensitivity. In a related project, we studied the relationship between growth rate and surface electrical currents in roots by examining the effects of gravity and thigmostimulation on surface potentials in maize roots.

Listeria monocytogenes - Danger for health safety vegetable production.

PubMed

Kljujev, Igor; Raicevic, Vera; Jovicic-Petrovic, Jelena; Vujovic, Bojana; Mirkovic, Milica; Rothballer, Michael

2018-04-22

The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L. monocytogenes in laboratory conditions. The detection of Listeria spp. and L. monocytogenes in vegetable samples was done using ISO and PCR methods. The investigation of colonization vegetable roots and detection Listeria-cells inside plant root tissue was done using Fluorescence in situ hybridization (FISH) method in combination with confocal laser scanning microscopy (CLSM). The results showed that 25.58% vegetable samples were positive for Listeria spp. and only one sample (carrot) was positive for L. monocytogenes out of 43 samples in total collected from field and greenhouse. The strain L. monocytogenes EGD-E surface and endophytic colonized carrot root in highest degree while strain L. monocytogenes SV4B was the most represented at leafy vegetable plants, such at lettuce (1.68 × 10 6  cells/mm 3 absolutely dry root) and spinach (1.39 × 10 6  cells/mm 3 absolutely dry root) root surface. The cells of L. monocytogenes SV4B were visible as single cells in interior tissue of plant roots (celery and sweet corn roots) as well as in the interior of the plant root cell at sweet corn root. The cells of L. monocytogenes EGD-E bind to the surface of the plant root and they were less commonly found out on root hair. In the inner layers of the root, those bacterial cells were inhabited intercellular spaces mainly as single cells very close to the larval vessels of root. Our results suggest that L. monocytogenes is very good endophytic colonizer of vegetable plant roots. Copyright © 2018 Elsevier Ltd. All rights reserved.

Highly concentrated EDTA gel improves cleaning efficiency of root canal preparation in vitro.

PubMed

Putzer, P; Hoy, L; Günay, H

2008-12-01

Debris and smear layer, as a product of mechanical root canal instrumentation, reduce the effectiveness of pharmacological substances to prevent post-treatment diseases and impair direct contact of filling materials with a clean dentinal surface. The aim of this in vitro study was to investigate the presence and localization of debris and smear layer via scanning electron microscope analysis after standardized root canal preparation with different chelating agents. Dentin surfaces received treatment with: (1) 15% ethylenediaminetetraacetic acid (EDTA), (2) 18.6% EDTA (3) and 24% EDTA or without any demineralizing chemicals as control. Forty vertically split human premolars were sputtered and divided into coronal, middle, and apical sections, followed by a randomized, blinded score evaluation using five scores. Pairwise comparisons of all treatment groups against a control group have been performed by Mann-Whitney U test and the Kruskal-Wallis test. Debris grades showed no significant difference between the three regions of the root canals, except for 18.6% EDTA in the central third. Smear layer and smear plug removal was concentration-dependent. Removal of the smear layer in the three areas showed that there was a statistically significant difference between all parts when using 18.6% and 24% EDTA concentrations compared with the control. The best smear layer removal in the apical region was observed using a 24% EDTA gel as chelating agent and lubricant. The usage of EDTA gel >/=18.6% presented a better cleaning regime when compared to the control group.

Modulatory effects by neodymium-doped yttrium aluminum garnet laser on fibroblast attachment to single rooted tooth surfaces following ultrasonic scaling and root planning: An in vitro study

PubMed Central

Negi, Shanta; Krishnamurthy, Malathi; Ganji, Kiran Kumar; Pendor, Sunil

2015-01-01

Context: One of the most important goals of periodontal therapy is connective tissue reattachment to previously diseased root surfaces. In the recent years, laser therapy has been considered as an important tool in improving the treatment of periodontal disease. Aims: To evaluate the neodymium-doped yttrium aluminum garnet (Nd: YAG) lasers effects on root surfaces affected by periodontal disease and compare this treatment with scaling and root planning (SRP) in terms of fibroblast attachment. Materials and Methods: A sample of 30 single-rooted human teeth extracted because of advanced periodontal disease was used in this study. Sixty specimens obtained by longitudinal sectioning were randomly divided in three groups. Group A control (untreated); Group B SRP; Group C laser (Nd: YAG) and ultrasonic scaling. All specimens were incubated with fibroblast suspension and then fixed and observed under scanning electron microscope. Results: With a median of 8, the control group (Group A) exhibited the least number of total fibroblasts among all the three groups. The laser and scaling - treated group (Group C) showed the highest number of fibroblasts (median = 49, mean ± standard deviation [SD] = 48.28 ± 17.18), followed by SRP only (Group B, median = 22, mean ± SD = 22.24 ± 8.67). Conclusions: Nd: YAG laser irradiation at specific energy densities can be used as a useful tool to condition the root surfaces, enhancing fibroblast attachment. Hence aiding in re-establishment of the connective tissue attachment to the root surfaces of previously diseased teeth. PMID:25810589

Root Surface Biomodification with Nd:YAG Laser for the Treatment of Gingival Recession with Subepithelial Connective Tissue Grafts

PubMed Central

Aydin, Tugba; Canakci, Varol; Cicek, Yasin

2010-01-01

Abstract Background/Aim: Root surface biomodification has been used to treat gingival recession and periodontitis. The principle for this procedure is that removing the smear layer from the root surfaces exposes collagen fibers, which leads to improved healing. Clinical studies generally have failed to find any improvement in clinical parameters when using such procedures, however. The aim of this study was to evaluate and compare the outcome of gingival recession therapy using the subepithelial connective tissue graft (SCTG) with or without Nd:YAG laser application for root surface biomodification. Materials and Methods: Thirty-four teeth in 17 patients with Miller Class 1 and 2 recession were treated with SCTG with (test group) or without (control group) the application of Nd:YAG laser (1 W, 10 Hz, 100 mj, 60 s, 1064 nm). Clinical attachment level (CAL), recession depth (RD), recession width (RW), and probing depth (PD) were measured at baseline and six months postsurgery. Results: Both treatments yielded significant improvements in terms of RD and RW decrease and CAL gain compared to baseline values. For test and control groups, the average root coverage was 33% and 77%, respectively (p < 0.05), and the complete root coverage was 18% and 65%, respectively (p < 0.05). The control group showed a greater reduction in RD and RW compared with the test group (p < 0.05). Conclusions: The use of Nd:YAG laser as a root surface biomodifier negatively affected the outcome of root coverage with the SCTG. PMID:19860567

Interaction of Escherichia coli with growing salad spinach plants.

PubMed

Warriner, Keith; Ibrahim, Faozia; Dickinson, Matthew; Wright, Charles; Waites, William M

2003-10-01

In this study, the interaction of a bioluminescence-labeled Escherichia coli strain with growing spinach plants was assessed. Through bioluminescence profiles, the direct visualization of E. coli growing around the roots of developing seedlings was accomplished. Subsequent in situ glucuronidase (GUS) staining of seedlings confirmed that E. coli had become internalized within root tissue and, to a limited extent, within hypocotyls. When inoculated seeds were sown in soil microcosms and cultivated for 42 days, E. coli was recovered from the external surfaces of spinach roots and leaves as well as from surface-sterilized roots. When 20-day-old spinach seedlings (from uninoculated seeds) were transferred to soil inoculated with E. coli, the bacterium became established on the plant surface, but internalization into the inner root tissue was restricted. However, for seedlings transferred to a hydroponic system containing 10(2) or 10(3) CFU of E. coli per ml of the circulating nutrient solution, the bacterium was recovered from surface-sterilized roots, indicating that it had been internalized. Differences between E. coli interactions in the soil and those in the hydroponic system may be attributed to greater accessibility of the roots in the latter model. Alternatively, the presence of a competitive microflora in soil may have restricted root colonization by E. coli. The implications of this study's findings with regard to the microbiological safety of minimally processed vegetables are discussed.

[Different responses of growth and root development of Schima superba provenance to the adjacent plant competition in different nutrient conditions].

PubMed

Yao, Jia Bao; Chu, Xiu Li; Zhou, Zhi Chun; Tong, Jian She; Wang, Hui; Yu, Jia Zhong

2017-04-18

Growth and root development of three Schima superba seedling provenances were influenced by adjacent plant competition in the homogeneous and heterogeneous nutrient environment, which revealed the reasons of S. superba competition differences in the different genotypes. The results indicated that, compared with homogeneous nutrient environment, all three S. superba provenances showed higher seedling height, more dry matter accumulation, and significant root proliferation in heterogeneous nutrient environment. Under heterogeneous nutrient environment, the seedlings of S. superba from Jian'ou of Fujian exhibited higher competitive advantage in growth than that of S. superba from Longquan of Zhejiang and Xinfeng of Jiangxi, especially standing out under mixed cultivation with seedlings of Cunninghamia lanceolata. Under mixed cultivation, the root growth parameters of S. superba such as root length, root surface area and volume increased by 20.4%-69.0% compared with the single plant, which enhanced the foraging ability and growth advantage. To some extent, however, the root development in Longquan of Zhejiang and Xinfeng of Jiangxi was suppressed when subjected to the pattern of mixed cultivation. Besides, the root growth and development of all three S. superba provenances were suppressed, which might be due to their root self-recognition in the pattern of mono cultivation. Therefore, the seedling growth in Jian'ou of Fujian decreased significantly, but seedling growth in Longquan of Zhejiang and Xinfeng of Jiangxi was not suppressed, even increased evidently, as their root physiological plasticity might play the crucial role in seedling growth. Hence the S. superba from Jian'ou of Fujian with high foraging efficiency and competition ability was suggested with the method of mixed forestation to improve the S. superba forest plantation productivity.

Diagnostic accuracy of cone-beam computed tomography scans with high- and low-resolution modes for the detection of root perforations.

PubMed

Shokri, Abbas; Eskandarloo, Amir; Norouzi, Marouf; Poorolajal, Jalal; Majidi, Gelareh; Aliyaly, Alireza

2018-03-01

This study compared the diagnostic accuracy of cone-beam computed tomography (CBCT) scans obtained with 2 CBCT systems with high- and low-resolution modes for the detection of root perforations in endodontically treated mandibular molars. The root canals of 72 mandibular molars were cleaned and shaped. Perforations measuring 0.2, 0.3, and 0.4 mm in diameter were created at the furcation area of 48 roots, simulating strip perforations, or on the external surfaces of 48 roots, simulating root perforations. Forty-eight roots remained intact (control group). The roots were filled using gutta-percha (Gapadent, Tianjin, China) and AH26 sealer (Dentsply Maillefer, Ballaigues, Switzerland). The CBCT scans were obtained using the NewTom 3G (QR srl, Verona, Italy) and Cranex 3D (Soredex, Helsinki, Finland) CBCT systems in high- and low-resolution modes, and were evaluated by 2 observers. The chi-square test was used to assess the nominal variables. In strip perforations, the accuracies of low- and high-resolution modes were 75% and 83% for NewTom 3G and 67% and 69% for Cranex 3D. In root perforations, the accuracies of low- and high-resolution modes were 79% and 83% for NewTom 3G and was 56% and 73% for Cranex 3D. The accuracy of the 2 CBCT systems was different for the detection of strip and root perforations. The Cranex 3D had non-significantly higher accuracy than the NewTom 3G. In both scanners, the high-resolution mode yielded significantly higher accuracy than the low-resolution mode. The diagnostic accuracy of CBCT scans was not affected by the perforation diameter.

[Effects of Nd: YAG laser irradiation on the root surfaces and adhesion of Streptococcus mutans].

PubMed

Yuanhong, Li; Zhongcheng, Li; Mengqi, Luo; Daonan, Shen; Shu, Zhang; Shu, Meng

2016-12-01

This study aimed to evaluate the effects of treatment with different powers of Nd: YAG laser irradiation on root surfaces and Streptococcus mutans (S. mutans) adhesion. Extracted teeth because of severe periodontal disease were divided into the following four groups: control group, laser group 1, laser group 2, and laser group 3. After scaling and root planning, laser group 1, laser group 2, and laser group 3 were separately treated with Nd: YAG laser irradiation (4/6/8 W, 60 s); however, the control group did not receive the treatment. Scanning electron microscopy (SEM) was used to determine the morphology. S. mutans were cultured with root slices from each group. Colony forming unit per mL (CFU·mL⁻¹) was used to count and compare the amounts of bacteria adhesion among groups. SEM was used to observe the difference of bacteria adhesion to root surfaces between control group (scaling) and laser group 2 (6 W, 60 s), thereby indicating the different bacteria adhesions because of different treatments. Morphology alterations indicated that root surfaces in control group contain obvious smear layer, debris, and biofilm; whereas the root surfaces in laser group contain more cracks with less smear layer and debris. The bacteria counting indicated that S. mutans adhesion to laser group was weaker than that of control group (P<0.05). No statistical significance among the laser groups (P>0.05) was observed. Morphology alterations also verified that S. mutans adhesion to laser group 2 (6 W, 60 s) was weaker than that of control group (scaling). This study demonstrated that Nd: YAG laser irradiation treatment after scaling can reduce smear layer, debris, and biofilm on the root surfaces as compared with conventional scaling. The laser treatment reduces the adhesion of S. mutans as well. However, Nd: YAG laser irradiation can cause cracks on the root surfaces. In this experiment, the optimum laser power of 6 W can thoroughly remove the smear layer and debris, as well as relatively improve the control of thermal damagee.

Houseplants, Indoor Air Pollutants, and Allergic Reactions

NASA Technical Reports Server (NTRS)

Wolverton, B. C.

1986-01-01

The technology of using houseplant leaves for reducing volatile organics inside closed facilities has been demonstrated with formaldehyde and benzene. Philodendrons are among the most effective plants tested to date. Philodendron domesticum had demonstrated the ability to remove formaldehyde from small experimental chambers at a rate of 4.31 micro-g/sq cm leaf surface area with initial starting concentrations of 22 ppm. At initial starting concentrations of 2.3 ppm a formaldehyde removal rate of 0.57 micro-g/sq cm was achieved during a 24 hour test. Aleo vera demonstrated a much higher formaldehyde efficiency removal rate than Philodendron domesticum at low formaldehyde concentrations. During a 24 hour exposure period 5 ppm of formaldehyde were reduced to 0.5 ppm demonstrating a removal efficiency rate of 3.27 micro-g/sq cm. Removal efficiency rates can be expected to decrease with concentration levels because fewer molecules of chemicals come in contact with the leaf surface area. Several centimeters of small washed gravel should be used to cover the surface of pot plants when large numbers of plants are kept in the home. The reason for this is to reduce the exposed area of damp potting soil which encourages the growth of molds (fungi). The leaves of Philodendron domesticum and golden pothos (Scindapsus aureus) have also demonstrated their ability to remove benzene and carbon monoxide from closed chambers. A combination of activated carbon and plant roots have demonstrated the greatest potential for removing large volumes of volatile organics along with smoke and possible radon from closed systems. Although fewer plants are required for this concept a mechanical blower motor must be used to pull or push the air through the carbon-root filter. NASA studies on motor sizes and bioregeneration rates should be completed by 1988.

A brief description of the simple biosphere model (SiB)

NASA Technical Reports Server (NTRS)

Sellers, P. J.; Mintz, Y.; Sud, Y. C.

1986-01-01

A biosphere model for calculating the transfer of energy, mass, and momentum between the atmosphere and the vegetated surface of the Earth was designed for atmospheric general circulation models. An upper vegetation layer represents the perennial canopy of trees or shrubs, a lower layer represents the annual ground cover of grasses and other herbacious species. The local coverage of each vegetation layer may be fractional or complete but as the individual vegetation elements are considered to be evenly spaced, their root systems are assumed to extend uniformly throughout the entire grid-area. The biosphere has seven prognostic physical-state variables: two temperatures (one for the canopy and one for the ground cover and soil surface); two interception water stores (one for the canopy and one for the ground cover); and three soil moisture stores (two of which can be reached by the vegetation root systems and one underlying recharge layer into and out of which moisture is transferred only by hydraulic diffusion).

Optimization of Fe2+ Removal from Coal Mine Wastewater using Activated Biochar of Colocasia esculenta.

PubMed

Banerjee, Soumya; LaminKa-Ot, Augustine; Joshi, S R; Mandal, Tamal; Halder, Gopinath

2017-09-01

  The present study investigates the sorptive removal of Fe2+ from simulated coal mine waste water using steam activated biochar (SABC) developed from the roots of Colocasia esculenta. The process was optimized by response surface methodology (RSM) under the influence of pH, temperature, adsorbent dosage and contact time at a constant shaking speed of 180 rpm with an initial concentration of 3 mg/L. The uptake performance of the biosorbent was assessed following a 24 full factorial experimental matrix developed by central composite design approach. Adsorbent was characterised by SEM, EDAX, XRD and B.E.T surface area analyzer. Maximum removal of 72.96% of Fe2+ was observed at pH 7.75, temperature 37.5 °C, adsorbent dosage 1.5 g/L for a time period of 180 mins. The study suggested that SABC prepared from roots of Colocasia esculenta could be used as an efficient and cost effective sorbent for removal of Fe2+ from coal mine wastewater.

GENETIC ANALYSIS OF THE AGGA LOCUS INVOLVED IN AGGLUTINATION ND ADHERENCE OF PSEUDOMONAS PUTIDA, A BENEFICIAL FLUORESCENT PSEUDOMONAD

EPA Science Inventory

An isolate of Pseudomonas putida, which rapidly adheres to plant roots is agglutinated by a glycoprotein from root surfaces. gglutination is presented and adherence to the root surface is diminished by Tn5 insertion in mutant 5123. wo cosmid clones from wild type P putida and 2.7...

ORCHIDEE-CNP: Site-Scale Evaluation against Observations from a Soil Formation Chronosequence in Hawaii

NASA Astrophysics Data System (ADS)

Goll, D. S.; Vuichard, N.; Maignan, F.; Jornet-Puig, A.; Sardans, J.; Peng, S.; Sun, Y.; Kvakić, M.; Guimberteau, M.; Guenet, B.; Zaehle, S.; Penuelas, J.; Jannssens, I.; Ciais, P.

2017-12-01

Land surface models rarely incorporate the terrestrial phosphorus cycle and its interactions with the carbon cycle, despite the extensive scientific debate about the importance of nitrogen and phosphorus supply for future land carbon uptake. We describe a representation of the terrestrial phosphorus cycle for the land surface model ORCHIDEE, and evaluate it with data from nutrient manipulation experiments along a soil formation chronosequence in Hawaii. ORCHIDEE accounts for influence of nutritional state of vegetation on tissue nutrient concentrations, photosynthesis, plant growth, biomass allocation, biochemical (phosphatase-mediated) mineralization and biological nitrogen fixation. Changes in nutrient content (quality) of litter affect the carbon use efficiency of decomposition and in return the nutrient availability to vegetation. The model explicitly accounts for root zone depletion of phosphorus as a function of root phosphorus uptake and phosphorus transport from soil to the root surface. The model captures the observed differences in the foliage stoichiometry of vegetation between an early (300yr) and a late stage (4.1 Myr) of soil development. The contrasting sensitivities of net primary productivity to the addition of either nitrogen, phosphorus or both among sites are in general reproduced by the model. As observed, the model simulates a preferential stimulation of leaf level productivity when nitrogen stress is alleviated, while leaf level productivity and leaf area index are stimulated equally when phosphorus stress is alleviated. The nutrient use efficiencies in the model are lower as observed primarily due to biases in the nutrient content and turnover of woody biomass.

Histologic comparison of the CO2 laser and Nd:YAG with and without water/air surface cooling on tooth root structure

NASA Astrophysics Data System (ADS)

Cobb, Charles M.; Spencer, Paulette; McCollum, Mark H.

1995-05-01

Specimens consisted of 18 extracted single rooted teeth unaffected by periodontal disease. After debriding roots, specimens were randomly divided into 4 treatment groups and subjected to a single pass, at varying energy densities, of a CO2, Nd:YAG, and Nd:YAG with air/water surface cooling (Nd:YAG-C). The rate of exposure was controlled at 4 mm/sec. Approximate energy densities were: CO2, 138, 206, 275, and 344 J/cm2; Nd:YAG, 114, 171, 229, and 286 J/cm2; Nd:YAG-C, 286, 343, 514, and 571 J/cm2. The CO2 laser was used both in continuous and pulsed beam modes (20 Hz, 0.01 sec pulse length and 0.8 mm dia spot size) whereas the Nd:YAG and Nd:YAG-C were preset at 50 Hz, 0.08 sec pulse length and 0.6 mm dia spot size. Specimen examination by SEM revealed, for all lasers, a direct correlation between increasing energy densities and depth of tissue ablation and width of tissue damage. However, to achieve the same relative dept of tissue ablation, the Nd:YAG-C required higher energy densities than either the CO2 or Nd:YAG lasers. The Nd:YAG-C generated a cavitation with sharply defined margins. Furthermore, regardless of energy density, and in contrast with other laser types, areas treated with the Nd:YAG-C did not exhibit collateral zones of heat damaged surface tissue.

Prior-knowledge-based spectral mixture analysis for impervious surface mapping

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Jinshui; He, Chunyang; Zhou, Yuyu

2014-01-03

In this study, we developed a prior-knowledge-based spectral mixture analysis (PKSMA) to map impervious surfaces by using endmembers derived separately for high- and low-density urban regions. First, an urban area was categorized into high- and low-density urban areas, using a multi-step classification method. Next, in high-density urban areas that were assumed to have only vegetation and impervious surfaces (ISs), the Vegetation-Impervious model (V-I) was used in a spectral mixture analysis (SMA) with three endmembers: vegetation, high albedo, and low albedo. In low-density urban areas, the Vegetation-Impervious-Soil model (V-I-S) was used in an SMA analysis with four endmembers: high albedo, lowmore » albedo, soil, and vegetation. The fraction of IS with high and low albedo in each pixel was combined to produce the final IS map. The root mean-square error (RMSE) of the IS map produced using PKSMA was about 11.0%, compared to 14.52% using four-endmember SMA. Particularly in high-density urban areas, PKSMA (RMSE = 6.47%) showed better performance than four-endmember (15.91%). The results indicate that PKSMA can improve IS mapping compared to traditional SMA by using appropriately selected endmembers and is particularly strong in high-density urban areas.« less

[Peculiarities of mitosis and nucleolar characteristics of the birch plantlets under antropogenous pollution].

PubMed

Butorina, A K; Kalaev, V N; Karpova, S S

2002-01-01

A study was made of some cytogenetic characteristics (mitotic activity, the level and spectrum of pathological mitosis, nucleolar features in root tip cells) in birch plantlets. The seeds were collected in four districts of Voronezh and in the ecologically clean territory. The index of mitotic activity has a considerable resistance to anthropogenous pollution. In the experimental areas, the level and spectrum of pathological mitosis increase. In contaminated areas we observed changes of nucleolar characteristics (the increased surface area of nucleoli and their higher number in cells, the increased number of cells with highly active types of nucleoli, the appearance of residual nucleoli). These changes can be considered as possible mechanisms of adaptation to stress due to antropogenous pollution. It is suggested that the use of such indices as single nucleolar surface area or the level of pathological mitosis may be perspective for cytogenetic monitoring of the environment, and for prognostification of environmental conditions suitable or unsuitable for the human health.

Vegetation death and rapid loss of surface elevation in two contrasting Mississippi delta salt marshes: The role of sedimentation, autocompaction and sea-level rise

USGS Publications Warehouse

Day, J.W.; Kemp, G.P.; Reed, D.J.; Cahoon, D.R.; Boumans, R.M.; Suhayda, J.M.; Gambrell, R.

2011-01-01

From 1990 to 2004, we carried out a study on accretionary dynamics and wetland loss in salt marshes surrounding two small ponds in the Mississippi delta; Old Oyster Bayou (OB), a sediment-rich area near the mouth of the Atchafalaya River and Bayou Chitigue (BC), a sediment-poor area about 70. km to the east. The OB site was stable, while most of the marsh at BC disappeared within a few years. Measurements were made of short-term sedimentation, vertical accretion, change in marsh surface elevation, pond wave activity, and marsh soil characteristics. The OB marsh was about 10. cm higher than BC; the extremes of the elevation range for Spartina alterniflora in Louisiana. Vertical accretion and short-term sedimentation were about twice as high at BC than at OB, but the OB marsh captured nearly all sediments deposited, while the BC marsh captured <30%. The OB and BC sites flooded about 15% and 85% of the time, respectively. Marsh loss at BC was not due to wave erosion. The mineral content of deposited sediments was higher at OB. Exposure and desiccation of the marsh surface at OB increased the efficiency that deposited sediments were incorporated into the marsh soil, and displaced the marsh surface upward by biological processes like root growth, while also reducing shallow compaction. Once vegetation dies, there is a loss of soil volume due to loss of root turgor and oxidation of root organic matter, which leads to elevation collapse. Revegetation cannot occur because of the low elevation and weak soil strength. The changes in elevation at both marsh sites are punctuated, occurring in steps that can either increase or decrease elevation. When a marsh is low as at BC, a step down can result in an irreversible change. At this point, the option is not restoration but creating a new marsh with massive sediment input either from the river or via dredging. ?? 2010 Elsevier B.V.

Method for producing smooth inner surfaces

DOEpatents

Cooper, Charles A.

2016-05-17

The invention provides a method for preparing superconducting cavities, the method comprising causing polishing media to tumble by centrifugal barrel polishing within the cavities for a time sufficient to attain a surface smoothness of less than 15 nm root mean square roughness over approximately a 1 mm.sup.2 scan area. The method also provides for a method for preparing superconducting cavities, the method comprising causing polishing media bound to a carrier to tumble within the cavities. The method also provides for a method for preparing superconducting cavities, the method comprising causing polishing media in a slurry to tumble within the cavities.

Adaptation of fine roots to annual fertilization and irrigation in a 13-year-old Pinus pinaster stand.

PubMed

Bakker, M R; Jolicoeur, E; Trichet, P; Augusto, L; Plassard, C; Guinberteau, J; Loustau, D

2009-02-01

Effects of fertilization and irrigation on fine roots and fungal hyphae were studied in 13-year-old maritime pine (Pinus pinaster Aït. in Soland), 7 years after the initiation of the treatments. The fertilization trials consisted of a phosphorus treatment, a complete fertilizer treatment (N, P, K, Ca and Mg), and an unfertilized treatment (control). Fertilizers were applied annually and were adjusted according to foliar target values. Two irrigation regimes (no irrigation and irrigation of a set amount each day) were applied from May to October. Root samples to depths of 120 cm were collected in summer of 2005, and the biomass of small roots (diameter 2-20 mm) and fine roots (diameter

Evaluation of LiDAR-acquired bathymetric and topographic data accuracy in various hydrogeomorphic settings in the Deadwood and South Fork Boise Rivers, West-Central Idaho, 2007

USGS Publications Warehouse

Skinner, Kenneth D.

2011-01-01

High-quality elevation data in riverine environments are important for fisheries management applications and the accuracy of such data needs to be determined for its proper application. The Experimental Advanced Airborne Research LiDAR (Light Detection and Ranging)-or EAARL-system was used to obtain topographic and bathymetric data along the Deadwood and South Fork Boise Rivers in west-central Idaho. The EAARL data were post-processed into bare earth and bathymetric raster and point datasets. Concurrently with the EAARL surveys, real-time kinematic global positioning system surveys were made in three areas along each of the rivers to assess the accuracy of the EAARL elevation data in different hydrogeomorphic settings. The accuracies of the EAARL-derived raster elevation values, determined in open, flat terrain, to provide an optimal vertical comparison surface, had root mean square errors ranging from 0.134 to 0.347 m. Accuracies in the elevation values for the stream hydrogeomorphic settings had root mean square errors ranging from 0.251 to 0.782 m. The greater root mean square errors for the latter data are the result of complex hydrogeomorphic environments within the streams, such as submerged aquatic macrophytes and air bubble entrainment; and those along the banks, such as boulders, woody debris, and steep slopes. These complex environments reduce the accuracy of EAARL bathymetric and topographic measurements. Steep banks emphasize the horizontal location discrepancies between the EAARL and ground-survey data and may not be good representations of vertical accuracy. The EAARL point to ground-survey comparisons produced results with slightly higher but similar root mean square errors than those for the EAARL raster to ground-survey comparisons, emphasizing the minimized horizontal offset by using interpolated values from the raster dataset at the exact location of the ground-survey point as opposed to an actual EAARL point within a 1-meter distance. The average error for the wetted stream channel surface areas was -0.5 percent, while the average error for the wetted stream channel volume was -8.3 percent. The volume of the wetted river channel was underestimated by an average of 31 percent in half of the survey areas, and overestimated by an average of 14 percent in the remainder of the survey areas. The EAARL system is an efficient way to obtain topographic and bathymetric data in large areas of remote terrain. The elevation accuracy of the EAARL system varies throughout the area depending upon the hydrogeomorphic setting, preventing the use of a single accuracy value to describe the EAARL system. The elevation accuracy variations should be kept in mind when using the data, such as for hydraulic modeling or aquatic habitat assessments.

Surface roughness and bacterial adhesion on root dentin treated with diode laser and conventional desensitizing agents.

PubMed

Cury, Maiza S; Silva, Camilla B; Nogueira, Ruchele D; Campos, Michelle G D; Palma-Dibb, Regina G; Geraldo-Martins, Vinicius R

2018-02-01

The treatments for dentin hypersensitivity (DH) may change the surface roughness of the root dentin, which can lead to biofilm accumulation, increasing the risk of root caries. The aim was to compare the surface roughness of root dentin after different treatments of DH and the biofilm formation on those surfaces. After initial surface roughness (Sa) assessment, 50 bovine root fragments received the following treatments (n = 10): G 1-no treatment; G2-5% sodium fluoride varnish; G3-professional application of a desensitizing dentifrice; G4-toothbrushing with a desensitizing dentifrice; and G5-diode laser application (908 nm; 1.5 W, 20 s). The Sa was reevaluated after treatments. Afterward, all samples were incubated in a suspension of Streptococcus mutans at 37 °C for 24 h. The colony-forming units (CFU) were counted using a stereoscope, and the results were expressed in CFU/mL. The one-way ANOVA and the Tukey's tests compared the roughness data and the results obtained on the bacterial adhesion test (α = 5%). G2 (2.3 ± 1.67%) showed similar Sa variation than G1 (0.25 ± 0.41%) and G5 (5.69 ± 0.99%), but different from group G3 (9.05 ± 2.39%). Group 4 showed the highest Sa variation (30.02 ± 3.83%; p < 0.05). Bacterial adhesion was higher in G4 (2208 ± 211.9), suggesting that bacterial growth is greater on rougher surfaces. The diode laser and the conventional treatments for DH may change the surface roughness of the root dentin, but only brushing with desensitizing dentifrice induced a higher bacteria accumulation on root dentin surface.

Surface correlations of hydrodynamic drag for transitionally rough engineering surfaces

NASA Astrophysics Data System (ADS)

Thakkar, Manan; Busse, Angela; Sandham, Neil

2017-02-01

Rough surfaces are usually characterised by a single equivalent sand-grain roughness height scale that typically needs to be determined from laboratory experiments. Recently, this method has been complemented by a direct numerical simulation approach, whereby representative surfaces can be scanned and the roughness effects computed over a range of Reynolds number. This development raises the prospect over the coming years of having enough data for different types of rough surfaces to be able to relate surface characteristics to roughness effects, such as the roughness function that quantifies the downward displacement of the logarithmic law of the wall. In the present contribution, we use simulation data for 17 irregular surfaces at the same friction Reynolds number, for which they are in the transitionally rough regime. All surfaces are scaled to the same physical roughness height. Mean streamwise velocity profiles show a wide range of roughness function values, while the velocity defect profiles show a good collapse. Profile peaks of the turbulent kinetic energy also vary depending on the surface. We then consider which surface properties are important and how new properties can be incorporated into an empirical model, the accuracy of which can then be tested. Optimised models with several roughness parameters are systematically developed for the roughness function and profile peak turbulent kinetic energy. In determining the roughness function, besides the known parameters of solidity (or frontal area ratio) and skewness, it is shown that the streamwise correlation length and the root-mean-square roughness height are also significant. The peak turbulent kinetic energy is determined by the skewness and root-mean-square roughness height, along with the mean forward-facing surface angle and spanwise effective slope. The results suggest feasibility of relating rough-wall flow properties (throughout the range from hydrodynamically smooth to fully rough) to surface parameters.

Inconsistency in the Crown-to-Root Ratios of Single-Rooted Premolars Measured by 2D and 3D Examinations.

PubMed

Hong, Hsiang-Hsi; Liu, Heng-Liang; Hong, Adrienne; Chao, Pu

2017-11-28

Micro-computed tomography (micro-CT) was applied to elucidate the relationship between the three-dimensional (3D) root surface area (RSA) and two-dimensional (2D) crown-to-root ratio (CRR) of extracted teeth to classify the periodontitis and assign a periodontal/prosthetic prognosis. A total of 31 maxillary and 35 mandibular single-rooted human premolars were examined. The amount of periodontal support on the basis of 3D RSA and 2D root length (RL) at CRRs of 1:1, 5:4, 3:2, and 2:1 were analyzed. Both maxillary and mandibular premolars demonstrated a nonsignificant RSA percentage at the evaluated CRRs. The coronal 21%-22% 2D RL and the 26%-28% 3D RSA bone loss apical to the cemento-enamel junction corresponded to a CRR of 1:1, relating to mild-moderate periodontitis. The coronal 30%-31% 2D RL and the 41%-42% 3D RSA bone loss corresponded to a CRR of 5:4, correlating to severe periodontitis. More severe clinical attachment loss (CAL) was observed in the 3D RSA measurement than in the 2D RL measurement at the evaluated CRRs. The amount of CAL at the CRR of 1:1 was inadequate to assess the severity of periodontitis on the basis of the 2D RL and 3D RSA measurements.

A Novel Growing Device Inspired by Plant Root Soil Penetration Behaviors

PubMed Central

Sadeghi, Ali; Tonazzini, Alice; Popova, Liyana; Mazzolai, Barbara

2014-01-01

Moving in an unstructured environment such as soil requires approaches that are constrained by the physics of this complex medium and can ensure energy efficiency and minimize friction while exploring and searching. Among living organisms, plants are the most efficient at soil exploration, and their roots show remarkable abilities that can be exploited in artificial systems. Energy efficiency and friction reduction are assured by a growth process wherein new cells are added at the root apex by mitosis while mature cells of the root remain stationary and in contact with the soil. We propose a new concept of root-like growing robots that is inspired by these plant root features. The device penetrates soil and develops its own structure using an additive layering technique: each layer of new material is deposited adjacent to the tip of the device. This deposition produces both a motive force at the tip and a hollow tubular structure that extends to the surface of the soil and is strongly anchored to the soil. The addition of material at the tip area facilitates soil penetration by omitting peripheral friction and thus decreasing the energy consumption down to 70% comparing with penetration by pushing into the soil from the base of the penetration system. The tubular structure provides a path for delivering materials and energy to the tip of the system and for collecting information for exploratory tasks. PMID:24587244

Assimilation of SMOS Brightness Temperatures or Soil Moisture Retrievals into a Land Surface Model

NASA Technical Reports Server (NTRS)

De Lannoy, Gabrielle J. M.; Reichle, Rolf H.

2016-01-01

Three different data products from the Soil Moisture Ocean Salinity (SMOS) mission are assimilated separately into the Goddard Earth Observing System Model, version 5 (GEOS-5) to improve estimates of surface and root-zone soil moisture. The first product consists of multi-angle, dual-polarization brightness temperature (Tb) observations at the bottom of the atmosphere extracted from Level 1 data. The second product is a derived SMOS Tb product that mimics the data at a 40 degree incidence angle from the Soil Moisture Active Passive (SMAP) mission. The third product is the operational SMOS Level 2 surface soil moisture (SM) retrieval product. The assimilation system uses a spatially distributed ensemble Kalman filter (EnKF) with seasonally varying climatological bias mitigation for Tb assimilation, whereas a time-invariant cumulative density function matching is used for SM retrieval assimilation. All assimilation experiments improve the soil moisture estimates compared to model-only simulations in terms of unbiased root-mean-square differences and anomaly correlations during the period from 1 July 2010 to 1 May 2015 and for 187 sites across the US. Especially in areas where the satellite data are most sensitive to surface soil moisture, large skill improvements (e.g., an increase in the anomaly correlation by 0.1) are found in the surface soil moisture. The domain-average surface and root-zone skill metrics are similar among the various assimilation experiments, but large differences in skill are found locally. The observation-minus-forecast residuals and analysis increments reveal large differences in how the observations add value in the Tb and SM retrieval assimilation systems. The distinct patterns of these diagnostics in the two systems reflect observation and model errors patterns that are not well captured in the assigned EnKF error parameters. Consequently, a localized optimization of the EnKF error parameters is needed to further improve Tb or SM retrieval assimilation.

Effect of Er:YAG laser-activated irrigation solution on Enterococcus Faecalis biofilm in an ex-vivo root canal model.

PubMed

Sahar-Helft, Sharonit; Stabholtz, Adam; Moshonov, Joshua; Gutkin, Vitaly; Redenski, Idan; Steinberg, Doron

2013-07-01

Abstract Objective: The purpose of this study was to evaluate mineral content and surface morphology of root canals coated with Enterococcus faecalis biofilm after treatment with several endodontic irrigation solutions, with and without Er:YAG laser-activated irrigation (LAI). LAI has been introduced as a powerful method for root canal irrigation resulting in smear-layer removal from the root canal wall. Distal and palatal roots from 60 freshly extracted human molars were used in this study. The coronal of each tooth was removed. Roots were split longitudinally and placed in an ultrasonic bath to remove the smear layer, creating conditions for the formation of E. faecalis biofilm. After incubation, the two halves were reassembled in impression material to simulate clinical conditions. Specimens were divided into two main groups: roots rinsed with irrigation solutions and roots subjected to laser irradiation combined with irrigation solutions. Solutions tested were 2% chlorhexidine and 17% ethylenediaminetetraacetic acid (EDTA) and saline. Surface morphology: 17% EDTA irrigant solution combined with Er:YAG laser showed the best results for removing bacteria from the root canal walls. Chemical analysis: all samples treated with combined laser irradiation and irrigation solution had low surface levels of Ca compared with samples treated with irrigation alone. The Ca/P ratio was highest in the laser-EDTA group. Overall, mineral changes caused by laser with irrigation solutions were minimal, and statistically nonsignificant. In vitro irrigation solutions, combined with Er:YAG laser irradiation, were effective in removing E. faecalis biofilm from root canal walls. Irrigation solutions without laser irradiation were less effective, leaving a layer of biofilm on the dentin surface.

Scanning electron microscopic study of the effects of Er:YAG laser on root cementum.

PubMed

Fujii, T; Baehni, P C; Kawai, O; Kawakami, T; Matsuda, K; Kowashi, Y

1998-11-01

Use of Er:YAG laser has been proposed for the removal of microbial deposits and calculus present on teeth affected by periodontal disease. However, the influence of Er:YAG laser irradiation on root surfaces has not yet been fully investigated. The aim of the present study was to evaluate the effects of Er:YAG laser irradiation on root cementum by scanning electron microscopy (SEM). Specimens were obtained from extracted human periodontally-diseased teeth using a water-cooled high-speed bur. An Er:YAG laser beam was then applied at various powers ranging from 25 to 100 mJ/ pulse/sec. The laser irradiation was performed under water irrigation, with the tip held perpendicular to the root surface in the contact mode. Following laser exposure, specimens were fixed, dehydrated, and dried at critical-point in liquid CO2. After mounting on SEM plates and sputter-coating with gold, the cementum surface was examined by SEM. Observations of the root surface showed a relatively flat surface in control specimens. In Er:YAG exposed specimens, the laser beam created a circular, notched-edge, crater-like defect on the root. The bottom of the lesion showed an irregular and sharp-pointed surface. Subsequently, the specimens were fractured with a sharp scalpel perpendicularly to the surface. SEM observations of these specimens showed a 15 microm layer of damaged tissue within the laser-irradiated cementum. The tissue presented an amorphous appearance and the Sharpey's and matrix fiber bundles were not clearly distinguishable. These observations indicate that cementum tissue could be damaged by Er:YAG laser irradiation.

Effect of restoration technique on stress distribution in roots with flared canals: an FEA study.

PubMed

Belli, Sema; Eraslan, Öznur; Eraslan, Oğuz; Eskitaşcıoğlu, Gürcan

2014-04-01

The aim of this finite element analysis (FEA) study was to test the effect of different restorative techniques on stress distribution in roots with flared canals. Five three-dimensional (3D) FEA models that simulated a maxillary incisor with excessive structure loss and flared root canals were created and restored with the following techniques/materials: 1) a prefabricated post: 2) one main and two accessory posts; 3) i-TFC post-core (Sun Medical); 4) the thickness of the root was increased by using composite resin and the root was then restored using a prefabricated post; 5) an anatomic post was created by using composite resin and a prefabricated glass-fiber post. Composite cores and ceramic crowns were created. A 300-N static load was applied at the center of the palatal surface of the tooth to calculate stress distributions. SolidWorks/Cosmosworks structural analysis programs were used for FEA analysis. The analysis of the von Mises and tensile stress values revealed that prefabricated post, accessory post, and i-TFC post systems showed similar stress distributions. They all showed high stress areas at the buccal side of the root (3.67 MPa) and in the cervical region of the root (> 3.67 MPa) as well as low stress accumulation within the post space (0 to 1 MPa). The anatomic post kept the stress within its body and directed less stress towards the remaining tooth structure. The creation of an anatomic post may save the remaining tooth structure in roots with flared canals by reducing the stress levels.

Enhancing phosphorus and zinc acquisition efficiency in rice: a critical review of root traits and their potential utility in rice breeding.

PubMed

Rose, T J; Impa, S M; Rose, M T; Pariasca-Tanaka, J; Mori, A; Heuer, S; Johnson-Beebout, S E; Wissuwa, M

2013-07-01

Rice is the world's most important cereal crop and phosphorus (P) and zinc (Zn) deficiency are major constraints to its production. Where fertilizer is applied to overcome these nutritional constraints it comes at substantial cost to farmers and the efficiency of fertilizer use is low. Breeding crops that are efficient at acquiring P and Zn from native soil reserves or fertilizer sources has been advocated as a cost-effective solution, but would benefit from knowledge of genes and mechanisms that confer enhanced uptake of these nutrients by roots. This review discusses root traits that have been linked to P and Zn uptake in rice, including traits that increase mobilization of P/Zn from soils, increase the volume of soil explored by roots or root surface area to recapture solubilized nutrients, enhance the rate of P/Zn uptake across the root membrane, and whole-plant traits that affect root growth and nutrient capture. In particular, this review focuses on the potential for these traits to be exploited through breeding programmes to produce nutrient-efficient crop cultivars. Few root traits have so far been used successfully in plant breeding for enhanced P and Zn uptake in rice or any other crop. Insufficient genotypic variation for traits or the failure to enhance nutrient uptake under realistic field conditions are likely reasons for the limited success. More emphasis is needed on field studies in mapping populations or association panels to identify those traits and underlying genes that are able to enhance nutrient acquisition beyond the level already present in most cultivars.

317/319 Phytoremediation site monitoring report - 2009 growing season : final report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Negri, C .N.; Benda, P. L.; Gopalakrishnan, G.

2010-02-10

In 1999, Argonne National Laboratory (Argonne) designed and installed a series of engineered plantings consisting of a vegetative cover system and approximately 800 hybrid poplars and willows rooting at various predetermined depths. The plants were installed using various methods including Applied Natural Science's TreeWell{reg_sign} system. The goal of the installation was to protect downgradient surface and groundwater by intercepting the contaminated groundwater with the tree roots, removing moisture from the upgradient soil area, reducing water infiltration, preventing soil erosion, degrading and/or transpiring the residual volatile organic compounds (VOCs), and removing tritium from the subsoil and groundwater. This report presents themore » results of the monitoring activities conducted by Argonne's Energy Systems (ES) Division in the growing season of 2009. Monitoring of the planted trees began soon after the trees were installed in 1999 and has been conducted every summer since then. As the trees grew and consolidated their growth into the contaminated soil and groundwater, their exposure to the contaminants was progressively shown through tissue sampling. During the 2009 sampling campaign, VOC concentrations found in the French Drain area were in general consistent with or slightly lower than the 2008 results. Additionally, closely repeated, stand wide analyses showed contaminant fluctuations that may indicate short-term contaminant depletion in the area of interest of roots. This data will be useful to determine short-term removal rate by the trees. As in previous years, levels in the Hydraulic Control Area were close to background levels except for a few exceptions.« less

Effect of zoledronic acid used in the root surface treatment of late replanted teeth: a study in rats.

PubMed

Mori, Graziela Garrido; Janjacomo, Daniela Maria de Mendonça; Nunes, Daniele Clapes; Castilho, Lithiene Ribeiro

2010-01-01

This study evaluated the use of zoledronic acid, a resorption inhibitor, as a medication for root resorption treatment of late replanted teeth. Twenty-four maxillary right central incisors of rats were avulsed and kept dry for 30 min. Then, the teeth were divided into 2 groups. In group I, root surface was treated with 2% sodium fluoride for 20 min; in group II, 10-6M zoledronic acid solution was used for 20 min. All root canals were filled with calcium hydroxide. Next, teeth were replanted in their respective sockets. After 15 and 60 days post-replantation, the animals were killed and the anatomic pieces were obtained and prepared for microscopic and morphometric analyses. The results showed that zoledronic acid was capable of limiting the occurrence of root resorption and preserving cementum resorption. Further research must be performed to confirm the use of zoledronic acid in root surface treatment of late replanted teeth.

Undergraduate students introduction to manual and rotary root canal instrumentation.

PubMed

Leonardi, Denise Piotto; Haragushiku, Gisele Aihara; Tomazinho, Flavia Sens Fagundes; Furuse, Adilson Yoshio; Volpato, Lusiane; Baratto-Filho, Flares

2012-01-01

The aim of this study was to evaluate the performance of undergraduates in their first contact with manual and rotary root canal instrumentation. Forty-two students who had never worked on a root canal before instrumented 42 extracted lower-incisors. Participants were assigned to one of two groups: Rotary instrumentation or manual instrumentation. Pre- and post-operative computed tomography scans were obtained with a 3-dimensional dental imaging system. Starting and finishing times of preparation were recorded. The cross-sectional area of the root canal was analyzed with 2-mm-below-the-apex initial and final transverse images recorded through a digital imaging system and analyzed with software to measure the initial and final area of the root canal in mm(2). Data from the cross-sectional area of the root canal and time spent were subjected to the Mann-Whitney's U-test (p<0.05). The rotary instrumentation group showed smaller time for preparation (p=0.0204). No differences between rotary and manual instrumentation regarding the cross-sectional area of the root canal were observed (p=0.25). No accidents occurred. Undergraduate students showed good performance in their first contact with the manual and rotary instrumentation with regard to time spent and cross-sectional area of the root canal, with no operative accidents.

Cross-reactive antigens and lectin as determinants of symbiotic specificity in the Rhizobium-clover association.

PubMed Central

Dazzo, F B; Hubbell, D H

1975-01-01

Cross-reactive antigens of clover roots and Rhizobium trifolii were detected on their cell surfaces by tube agglutination, immunofluorescent, and radioimmunoassay techniques. Anti-clover root antiserum had a higher agglutinating titer with infective strains of R. trifolii than with noninfective strains. The root antiserum previously adsorbed with noninfective R. trifolii cells remained reactive only with infective cells, including infective revertants. When adsorbed with infective cells, the root antiserum was reactive with neither infective nor noninfective cells. Other Rhizobium species incapable of infecting clover did not demonstrate surface antigens cross-reactive with clover. Radioimmunoassay indicated twice as much antigenic cross-reactivity of clover roots and R. trifolii 403 (infective) than R. trifolii Bart A (noninfective). Immunofluorescence with anti-R. trifolii (infective) antiserum was detected on the exposed surface of the root epidermal cells and diminished at the root meristem. The immunofluorescent crossreaction on clover roots was totally removed by adsorption of anti-R. trifolii (infective) antiserum with encapsulated infective cells but not with noninfective cells. The cross-reactive capsular antigens from R. trifolii strains were extracted and purified. The ability of these antigens to induce clover root hair deformation was much greater when they were obtained from the infective than noninfective strains. The cross-reactive capsular antigen of R. trifolii 403 was characterized as a high-molecular-weight (greater than 4.6 times 10(6) daltons), beta-linked, acidic heteropolysaccharide containing 2-deoxyglucose, galactose, glucose, and glucuronic acid. A soluble, nondialyzable, substance (clover lectin) capable of binding to the cross-reactive antigen and agglutinating only infective cells of R. trifolii was extracted from white clover seeds. This lectin was sensitive to heat, Pronase, and trypsin. inhibition studies indicated that 2-deoxyglucose was the most probable haptenic determinant of the cross-reactive capsular antigen capable of binding to the root antiserum and the clover lectin. A model is proposed suggesting the preferential adsorption of infective versus noninfective cells of R. trifolii on the surface of clover roots by a cross-bridging of their common surface antigens with a multivalent clover lectin. Images PMID:55100

Evaluation of Temperature Elevation During Root Canal Treatment with Dual Wavelength Laser: 2780 nm Er,Cr:YSGG and 940 nm Diode.

PubMed

Al-Karadaghi, Tamara Sardar; Gutknecht, Norbert; Jawad, Hussein A; Vanweersch, Leon; Franzen, Rene

2015-09-01

The purpose of this study was to evaluate the effects of dual wavelength (2780 nm Er,Cr:YSGG and 940 nm diode) laser with radial firing tip (RFT) on the external root surface and sub-surfaces, in terms of temperature changes during laser-assisted root canal treatment. A significant factor that may limit the use of lasers in endodontics is the possible thermal injury to tooth supporting structures. A total of 50 sound single-rooted extracted teeth were divided randomly into two groups (n = 25). Group A, irradiated with Er,Cr:YSGG laser at 1.06 W, 50 Hz, and 50 μs was a control group, and group B was irradiated with dual wavelength of Er,Cr:YSGG laser with the same settings as group A and a diode laser of 0.51 W at 4 ms and 10 ms pulse duration. K-type thermocouples were used to record temperature changes at the cervical, middle, and apical root thirds, on root surfaces and sub-surfaces, arising from delivery of laser energy through RFT. Temperature elevation in group B was significantly higher in the middle and apical thirds of the prepared samples than in group A (p < 0.0001). Group B presented a mean temperature elevation of 5.07°C on the apical surface region corresponding to a 1.48 mm dentin thickness, whereas a mean temperature increase of 7.72°C was recorded corresponding to dentin thickness of 0.95 mm. Within the studied parameters, the dual wavelength laser did not result in adverse thermal changes on the external root surface in vitro.

Root surface temperature variation during mechanical removal of root canal filling material. An in vitro study.

PubMed

García-Cuerva, Martín; Horvath, Lucía; Pinasco, Laura; Ciparelli, Verónica; Gualtieri, Ariel; Casadoumecq, Ana C; Rodríguez, Pablo; Gonzalez-Zanotto, Carlos

2017-04-01

The aim of this study was to analyze in vitro temperature changes on the outer surface of the dental root during mechanical filling removal procedures. Thirty recently extracted single-rooted lower premolars were cut transversally at 16 mm from the apex in order to standardize sample length. Endodontic treatment was performed on them. The filling material was subsequently removed using Gates Glidden (G1, G2, G3); Peeso (P1, P2, P3) and PostecPlus FRC (FRC) reamers while temperatures were measured on the outer surface using a digital device with thermocouple at 0, 2, 4, 6, 8, 10 and 15 seconds. Temperatures were compared using repeated measures ANOVA followed by pairwise comparison with Tukey's test. All reamers caused significant temperature variation between different times (p<0.05). Pairwise comparisons indicated that temperature increased with time for all reamers (p<0.05). Significant differences in temperature were found between different reamers after 0, 2, 4, 6, 8,10 and 15 seconds (p<0.05). Temperature at the root surface increased considerably. Values higher than 50°C were recorded, the greatest increase from baseline being 16°C. Accordingly, if the procedure were begun at 37°C (physiological temperature), the temperature in the surrounding tissues - cementum, periodontium and bone - would rise to 53°C. An increase in 10°C above body temperature at the root surface may cause lesions in surrounding tissues. While removing filling material, it is essential to cool, control action time and use instruments in perfect condition, all of which may contribute to reducing the heat generated and transmitted to the outer root surface. Sociedad Argentina de Pediatría.

KSC-2009-2109

NASA Image and Video Library

2009-03-03

CAPE CANAVERAL, Fla. – At NASA's Kennedy Space Center in Florida, a wild pig stops hunting for food to eye the photographer. This pig is part of a larger family in the area. The wild pigs have flourished in the environs around Kennedy, which shares a border with the Merritt Island National Wildlife Refuge, without many predators other than panthers and humans. Pigs were introduced to Florida in the 1500s and are now found statewide in wooded areas close to water. Pigs are omnivores, foraging on the ground and rooting just beneath the surface, which damages the groundcover. Wild pigs eat almost anything that has nutritional value, including tubers, roots, shoots, acorns, fruits, berries, earthworms, amphibians, reptiles and rodents. Their appearance is similar to domestic hogs, but leaner, with a longer, narrower head and a coarser, denser coat. Females may have two litters per year. The piglets are weaned in a few weeks but remain with the mother for several months. Photo credit: NASA/Ben Smegelsky

Phytoremediation potential of Eichornia crassipes in metal-contaminated coastal water.

PubMed

Agunbiade, Foluso O; Olu-Owolabi, Bamidele I; Adebowale, Kayode O

2009-10-01

The potential of Eichornia crassipes to serve as a phytoremediation plant in the cleaning up of metals from contaminated coastal areas was evaluated in this study. Ten metals, As, Cd, Cu, Cr, Fe, Mn, Ni, Pb, V and Zn were assessed in water and the plant roots and shoots from the coastal area of Ondo State, Nigeria and the values were used to evaluate the enrichment factor (EF) and translocation factor (TF) in the plant. The critical concentrations of the metals were lower than those specified for hyperaccumulators thus classifying the plant as an accumulator but the EF and TF revealed that the plant accumulated toxic metals such as Cr, Cd, Pb and As both at the root and at the shoot in high degree, which indicates that the plant that forms a large biomass on the water surface and is not fed upon by animals can serve as a plant for both phytoextraction and rhizofiltration in phytoremediation technology.

Effects of damping-off caused by Rhizoctonia solani anastomosis group 2-1 on roots of wheat and oil seed rape quantified using X-ray Computed Tomography and real-time PCR

PubMed Central

Sturrock, Craig J.; Woodhall, James; Brown, Matthew; Walker, Catherine; Mooney, Sacha J.; Ray, Rumiana V.

2015-01-01

Rhizoctonia solani is a plant pathogenic fungus that causes significant establishment and yield losses to several important food crops globally. This is the first application of high resolution X-ray micro Computed Tomography (X-ray μCT) and real-time PCR to study host–pathogen interactions in situ and elucidate the mechanism of Rhizoctonia damping-off disease over a 6-day period caused by R. solani, anastomosis group (AG) 2-1 in wheat (Triticum aestivum cv. Gallant) and oil seed rape (OSR, Brassica napus cv. Marinka). Temporal, non-destructive analysis of root system architectures was performed using RooTrak and validated by the destructive method of root washing. Disease was assessed visually and related to pathogen DNA quantification in soil using real-time PCR. R. solani AG2-1 at similar initial DNA concentrations in soil was capable of causing significant damage to the developing root systems of both wheat and OSR. Disease caused reductions in primary root number, root volume, root surface area, and convex hull which were affected less in the monocotyledonous host. Wheat was more tolerant to the pathogen, exhibited fewer symptoms and developed more complex root systems. In contrast, R. solani caused earlier damage and maceration of the taproot of the dicot, OSR. Disease severity was related to pathogen DNA accumulation in soil only for OSR, however, reductions in root traits were significantly associated with both disease and pathogen DNA. The method offers the first steps in advancing current understanding of soil-borne pathogen behavior in situ at the pore scale, which may lead to the development of mitigation measures to combat disease influence in the field. PMID:26157449

Efficacy of ProTaper universal retreatment files in removing filling materials during root canal retreatment.

PubMed

Giuliani, Valentina; Cocchetti, Roberto; Pagavino, Gabriella

2008-11-01

The aim of this study was to evaluate the efficacy of the ProTaper Universal System rotary retreatment system and of Profile 0.06 and hand instruments (K-file) in the removal of root filling materials. Forty-two extracted single-rooted anterior teeth were selected. The root canals were enlarged with nickel-titanium (NiTi) rotary files, filled with gutta-percha and sealer, and randomly divided into 3 experimental groups. The filling materials were removed with solvent in conjunction with one of the following devices and techniques: the ProTaper Universal System for retreatment, ProFile 0.06, and hand instruments (K-file). The roots were longitudinally sectioned, and the image of the root surface was photographed. The images were captured in JPEG format; the areas of the remaining filling materials and the time required for removing the gutta-percha and sealer were calculated by using the nonparametric one-way Kruskal-Wallis test and Tukey-Kramer tests, respectively. The group that showed better results for removing filling materials was the ProTaper Universal System for retreatment files, whereas the group of ProFile rotary instruments yielded better root canal cleanliness than the hand instruments, even though there was no statistically significant difference. The ProTaper Universal System for retreatment and ProFile rotary instruments worked significantly faster than the K-file. The ProTaper Universal System for retreatment files left cleaner root canal walls than the K-file hand instruments and the ProFile Rotary instruments, although none of the devices used guaranteed complete removal of the filling materials. The rotary NiTi system proved to be faster than hand instruments in removing root filling materials.

Effects of damping-off caused by Rhizoctonia solani anastomosis group 2-1 on roots of wheat and oil seed rape quantified using X-ray Computed Tomography and real-time PCR.

PubMed

Sturrock, Craig J; Woodhall, James; Brown, Matthew; Walker, Catherine; Mooney, Sacha J; Ray, Rumiana V

2015-01-01

Rhizoctonia solani is a plant pathogenic fungus that causes significant establishment and yield losses to several important food crops globally. This is the first application of high resolution X-ray micro Computed Tomography (X-ray μCT) and real-time PCR to study host-pathogen interactions in situ and elucidate the mechanism of Rhizoctonia damping-off disease over a 6-day period caused by R. solani, anastomosis group (AG) 2-1 in wheat (Triticum aestivum cv. Gallant) and oil seed rape (OSR, Brassica napus cv. Marinka). Temporal, non-destructive analysis of root system architectures was performed using RooTrak and validated by the destructive method of root washing. Disease was assessed visually and related to pathogen DNA quantification in soil using real-time PCR. R. solani AG2-1 at similar initial DNA concentrations in soil was capable of causing significant damage to the developing root systems of both wheat and OSR. Disease caused reductions in primary root number, root volume, root surface area, and convex hull which were affected less in the monocotyledonous host. Wheat was more tolerant to the pathogen, exhibited fewer symptoms and developed more complex root systems. In contrast, R. solani caused earlier damage and maceration of the taproot of the dicot, OSR. Disease severity was related to pathogen DNA accumulation in soil only for OSR, however, reductions in root traits were significantly associated with both disease and pathogen DNA. The method offers the first steps in advancing current understanding of soil-borne pathogen behavior in situ at the pore scale, which may lead to the development of mitigation measures to combat disease influence in the field.

Histological changes induced by CO2 laser microprobe specially designed for root canal sterilization: in vivo study.

PubMed

Kesler, G; Koren, R; Kesler, A; Hay, N; Gal, R

1998-10-01

Until now, no suitable delivery fiber has existed for CO2 laser endodontic radiation in the apical region, where it is most difficult to eliminate the pulp tissue using conventional methods. To overcome this problem, we have designed a microprobe that reaches closer to the apex, distributing the energy density to a smaller area of the root canal and thus favorably increasing the thermal effects. A CO2 laser microprobe coupled onto a special hand piece was attached to the delivery fiber of a Sharplan 15-F CO2 laser. The study was conducted on 30 vital maxillary or mandibulary, central, lateral, or premolar teeth destined for extraction due to periodontal problems. Twenty were experimentally treated with pulsed CO2 laser delivered by this newly developed fiber after conventional root canal preparation. Temperature measured at three points on the root surface during laser treatment did not exceed 38 degrees C. Ten teeth represented the control group, in which only root canal preparation was performed in the conventional method. Histological examination of the laser-treated teeth showed coagulation necrosis and vacuolization of the remaining pulp tissue in the root canal periphery. Primary and secondary dentin appeared normal in all cases treated with 15-F CO2 laser. Gram stain and bacteriologic examination revealed complete sterilization. These results demonstrate the unique capabilities of this special microprobe in sterilization of the root canal, with no thermal damage to the surrounding tissue. The combination of classical root canal preparation with CO2 laser irradiation using this special microprobe before closing the canal can drastically change the quality of root canal fillings.

The mechanics of delamination in fiber-reinforced composite materials. Part 1: Stress singularities and solution structure

NASA Technical Reports Server (NTRS)

Wang, S. S.; Choi, I.

1983-01-01

The fundamental mechanics of delamination in fiber composite laminates is studied. Mathematical formulation of the problem is based on laminate anisotropic elasticity theory and interlaminar fracture mechanics concepts. Stress singularities and complete solution structures associated with general composite delaminations are determined. For a fully open delamination with traction-free surfaces, oscillatory stress singularities always appear, leading to physically inadmissible field solutions. A refined model is introduced by considering a partially closed delamination with crack surfaces in finite-length contact. Stress singularities associated with a partially closed delamination having frictional crack-surface contact are determined, and are found to be diferent from the inverse square-root one of the frictionless-contact case. In the case of a delamination with very small area of crack closure, a simplified model having a square-root stress singularity is employed by taking the limit of the partially closed delamination. The possible presence of logarithmic-type stress singularity is examined; no logarithmic singularity of any kind is found in the composite delamination problem. Numerical examples of dominant stress singularities are shown for delaminations having crack-tip closure with different frictional coefficients between general (1) and (2) graphite-epoxy composites.

Recharge at the Hanford Site: Status report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gee, G.W.

A variety of field programs designed to evaluate recharge and other water balance components including precipitation, infiltration, evaporation, and water storage changes, have been carried out at the Hanford Site since 1970. Data from these programs have indicated that a wide range of recharge rates can occur depending upon specific site conditions. Present evidence suggests that minimum recharge occurs where soils are fine-textured and surfaces are vegetated with deep-rooted plants. Maximum recharge occurs where coarse soils or gravels exist at the surface and soils are kept bare. Recharge can occur in areas where shallow-rooted plants dominate the surface, particularly wheremore » soils are coarse-textured. Recharge estimates have been made for the site using simulation models. A US Geological Survey model that attempts to account for climate variability, soil storage parameters, and plant factors has calculated recharge values ranging from near zero to an average of about 1 cm/yr for the Hanford Site. UNSAT-H, a deterministic model developed for the site, appears to be the best code available for estimating recharge on a site-specific basis. Appendix I contains precipitation data from January 1979 to June 1987. 42 refs., 11 figs., 11 tabs.« less

In-flight flow visualization results from the X-29A aircraft at high angles of attack

NASA Technical Reports Server (NTRS)

Delfrate, John H.; Saltzman, John A.

1992-01-01

Flow visualization techniques were used on the X-29A aircraft at high angles of attack to study the vortical flow off the forebody and the surface flow on the wing and tail. The forebody vortex system was studied because asymmetries in the vortex system were suspected of inducing uncommanded yawing moments at zero sideslip. Smoke enabled visualization of the vortex system and correlation of its orientation with flight yawing moment data. Good agreement was found between vortex system asymmetries and the occurrence of yawing moments. Surface flow on the forward-swept wing of the X-29A was studied using tufts and flow cones. As angle of attack increased, separated flow initiated at the root and spread outboard encompassing the full wing by 30 deg angle of attack. In general, the progression of the separated flow correlated well with subscale model lift data. Surface flow on the vertical tail was also studied using tufts and flow cones. As angle of attack increased, separated flow initiated at the root and spread upward. The area of separated flow on the vertical tail at angles of attack greater than 20 deg correlated well with the marked decrease in aircraft directional stability.

Fine Root Abundance and Dynamics of Stone Pine (Pinus cembra) at the Alpine Treeline Is Not Impaired by Self-shading.

PubMed

Kubisch, Petra; Leuschner, Christoph; Coners, Heinz; Gruber, Andreas; Hertel, Dietrich

2017-01-01

Low temperatures are crucial for the formation of the alpine treeline worldwide. Since soil temperature in the shade of tree canopies is lower than in open sites, it was assumed that self-shading may impair the trees' root growth performance. While experiments with tree saplings demonstrate root growth impairment at soil temperatures below 5-7°C, field studies exploring the soil temperature - root growth relationship at the treeline are missing. We recorded soil temperature and fine root abundance and dynamics in shaded and sun-exposed areas under canopies of isolated Pinus cembra trees at the alpine treeline. In contrast to the mentioned assumption, we found more fine root biomass and higher fine root growth in colder than in warmer soil areas. Moreover, colder areas showed higher fine root turnover and thus lower root lifespan than warmer places. We conclude that P. cembra balances enhanced fine root mortality in cold soils with higher fine root activity and by maintaining higher fine root biomass, most likely as a response to shortage in soil resource supply. The results from our study highlight the importance of in situ measurements on mature trees to understand the fine root response and carbon allocation pattern to the thermal growth conditions at the alpine treeline.

The effect of four different irrigation systems in the removal of a root canal sealer.

PubMed

Grischke, J; Müller-Heine, A; Hülsmann, M

2014-09-01

The aim of this study was to compare the efficiency of sonic, ultrasonic, and hydrodynamic devices in the removal of a root canal sealer from the surface and from simulated irregularities of root canals. Fifty-three root canals with two standardized grooves in the apical and coronal parts of longitudinally split roots were covered with AH Plus root canal sealer. Compared were the effects of (control) syringe irrigation, (1) CanalBrush, (2) passive ultrasonic irrigation, (3) EndoActivator, and (4) RinsEndo on the removal of the sealer. The specimens were divided into four groups (N = 12) and one control group (N = 5) via randomization. The amount of remaining sealer in the root canal irregularities was evaluated under a microscope using a 4-grade scoring system, whereas the remaining sealer on the root canal surface was evaluated with a 7-grade scoring system. Passive ultrasonic irrigation is more effective than the other tested irrigation systems or syringe irrigation in removing sealer from root canal walls (p < 0.01). None of the techniques had a significant effect on cleaning the lateral grooves. Within the limitations of this study protocol ultrasonic irrigation shows a superior effect on sealer removal from the root canal surface during endodontic retreatment. Cleaning of lateral grooves seems not to be possible with one of the techniques investigated. Incomplete removal of root canal sealer during re-treatment may cause treatment failure. Passive Ultrasonic irrigation seems to be the most effective system to remove sealer from a root canal.

Cold temperature delays wound healing in postharvest sugarbeet roots

USDA-ARS?s Scientific Manuscript database

Storage temperature affects the rate and extent of wound-healing in a number of root and tuber crops. The effect of storage temperature on wound-healing in sugarbeet (Beta vulgaris L.) roots, however, is largely unknown. Wound-healing of sugarbeet roots was investigated using surface-abraded roots s...

Implementing dynamic root optimization in Noah-MP for simulating phreatophytic root water uptake

USDA-ARS?s Scientific Manuscript database

Plants are known to adjust their root systems to adapt to changing subsurface water conditions. However, most current land surface models (LSMs) use a prescribed, static root profile, which cuts off the interactions between soil moisture and root dynamics. In this paper, we implemented an optimality...

Airborne Detection of Cosmic-Ray Albedo Neutrons for Regional-Scale Surveys of Root-Zone Soil Water on Earth

NASA Astrophysics Data System (ADS)

Schrön, M.; Bannehr, L.; Köhli, M.; Zreda, M. G.; Weimar, J.; Zacharias, S.; Oswald, S. E.; Bumberger, J.; Samaniego, L. E.; Schmidt, U.; Zieger, P.; Dietrich, P.

2017-12-01

While the detection of albedo neutrons from cosmic rays became a standard method in planetary space science, airborne neutron sensing has never been conceived for hydrological research on Earth. We assessed the applicability of atmospheric neutrons to sense root-zone soil moisture averaged over tens of hectares using neutron detectors on an airborne vehicle. Large-scale quantification of near-surface water content is an urgent challenge in hydrology. Information about soil and plant water is crucial to accurately assess the risks for floods and droughts, to adjust regional weather forecasts, and to calibrate and validate the corresponding models. However, there is a lack of data at scales relevant for these applications. Most conventional ground-based geophysical instruments provide root-zone soil moisture only within a few tens of m2, while electromagnetic signals from conventional remote-sensing instruments can only penetrate the first few centimeters below surface, though at larger spatial areas.In the last couple of years, stationary and roving neutron detectors have been used to sense the albedo component of cosmic-ray neutrons, which represents the average water content within 10—15 hectares and 10—50 cm depth. However, the application of these instruments is limited by inaccessible terrain and interfering local effects from roads. To overcome these limitations, we have pioneered first simulations and experiments of such sensors in the field of airborne geophysics. Theoretical investigations have shown that the footprint increases substantially with height above ground, while local effects smooth out throughout the whole area. Campaigns with neutron detectors mounted on a lightweight gyrocopter have been conducted over areas of various landuse types including agricultural fields, urban areas, forests, flood plains, and lakes. The neutron signal showed influence of soil moisture patterns in heights of up to 180 m above ground. We found correlation with ground-truthing data, using mobile cosmic-ray neutron sensors, local soil samples, TDR, and buried wireless soil moisture monitoring networks. The work opens the path towards further systematic assessment of airborne neutron sensing, which could become a valuable addition - or even an alternative - to conventional remote-sensing methods.

Tetralogy of Fallot and aortic root dilation: a long-term outlook.

PubMed

Nagy, Christian D; Alejo, Diane E; Corretti, Mary C; Ravekes, William J; Crosson, Jane E; Spevak, Philip J; Ringel, Richard; Carson, Kathryn A; Khalil, Sara; Dietz, Harry C; Cameron, Duke E; Vricella, Luca A; Traill, Thomas A; Holmes, Kathryn W

2013-04-01

Dilation of the sinus of Valsalva (SoV) has been increasingly observed after repaired tetralogy of Fallot (TOF). We estimate the prevalence of SoV dilation in adults with repaired TOF and analyze possible factors related to aortic disease. Adults with TOF [n = 109, median age 33.2 years (range 18.1 to 69.5)] evaluated at Johns Hopkins Hospital from 2001 to 2009 were reviewed in an observational retrospective cohort study. Median follow-up was 27.3 (range 0.1-48.8) years. SoV dilation was defined as >95 % confidence interval adjusted for age and body surface area (z-score > 2). The prevalence of SoV dilation was 51 % compared with that of a normal population with a mean z-score of 2.03. Maximal aortic diameters were ≥ 4 cm in 39 % (42 of 109), ≥ 4.5 cm in 21 % (23 of 109), ≥ 5 cm in 8 % (9 of 109), and ≥ 5.5 cm in 2 % (2 of 109). There was no aortic dissection or death due contributable to aortic disease. Aortic valve replacement was performed in 1.8 % and aortic root or ascending aorta (AA) replacement surgery in 2.8 % of patients. By multivariate logistic regression analysis, aortic regurgitation (AR) [odds ratio (OR) = 3.09, p = 0.005], residual ventricular septal defect (VSD) (OR = 4.14, p < 0.02), and TOF with pulmonary atresia (TOF/PA) (OR = 6.75, p = 0.03) were associated with increased odds of dilated aortic root. SoV dilation after TOF repair is common and persists with aging. AR, residual VSD, and TOF/PA are associated with increased odds of dilation. AA evaluation beyond the SoV is important. Indexed values are imperative to avoid bias on the basis of age and body surface area.

The Integrated Role of Water Availability, Nutrient Dynamics, and Xylem Hydraulic Dysfunction on Plant Rooting Strategies in Managed and Natural Ecosystems

NASA Astrophysics Data System (ADS)

Mackay, D. S.; Savoy, P.; Pleban, J. R.; Tai, X.; Ewers, B. E.

2015-12-01

Plants adapt or acclimate to changing environments in part by allocating biomass to roots and leaves to strike a balance between water and nutrient uptake requirements on the one hand and growth and hydraulic safety on the other hand. In a recent study examining experimental drought with the TREES model, which couples plant ecophysiology with rhizosphere-and-xylem hydraulics, we hypothesized that the asynchronous nature of soil water availability and xylem repair supported root-to-leaf area (RLA) proportionality that favored long-term survival over short-term carbon gain or water use. To investigate this as a possible general principal of plant adjustment to changing environmental conditions, TREES was modified to allocate carbon to fine and coarse roots organized in ten orders differing in biomass allocated per unit absorbing root area, root lifespan, and total absorbing root area in each of several soil-root zones with depth. The expanded model allowed for adjustment of absorbing root area and rhizosphere volume based on available carbohydrate production and nitrogen (N) availability, resulting in dynamic expansion and contraction of the supply-side of the rhizosphere-plant hydraulics and N uptake capacity in response to changing environmental conditions and plant-environment asynchrony. The study was conducted partly in a controlled experimental setting with six genotypes of a widely grown crop species, Brassica rapa. The implications for forests were investigated in controlled experiments and at Fluxnet sites representing temperate mixed forests, semi-arid evergreen needle-leaf, and Mediterranean biomes. The results showed that the effects of N deficiency on total plant growth was modulated by a relative increase in fine root biomass representing a larger absorbing root volume per unit biomass invested. We found that the total absorbing root area per unit leaf area was consistently lower than that needed to maximize short-term water uptake and carbohydrate gain. Moreover, the acclimated RLA fell within a small range for both crops and trees despite changing environmental conditions, demonstrating an adaptation that was consistent with empiricism on fine roots and thus pointing to a fundamental connection between ecological and hydrological processes.

Rhizosphere Bacteria Enhance Selenium Accumulation and Volatilization by Indian Mustard1

PubMed Central

de Souza, Mark P.; Chu, Dara; Zhao, May; Zayed, Adel M.; Ruzin, Steven E.; Schichnes, Denise; Terry, Norman

1999-01-01

Indian mustard (Brassica juncea L.) accumulates high tissue Se concentrations and volatilizes Se in relatively nontoxic forms, such as dimethylselenide. This study showed that the presence of bacteria in the rhizosphere of Indian mustard was necessary to achieve the best rates of plant Se accumulation and volatilization of selenate. Experiments with the antibiotic ampicillin showed that bacteria facilitated 35% of plant Se volatilization and 70% of plant tissue accumulation. These results were confirmed by inoculating axenic plants with rhizosphere bacteria. Compared with axenic controls, plants inoculated with rhizosphere bacteria had 5-fold higher Se concentrations in roots (the site of volatilization) and 4-fold higher rates of Se volatilization. Plants with bacteria contained a heat-labile compound in their root exudate; when this compound was added to the rhizosphere of axenic plants, Se accumulation in plant tissues increased. Plants with bacteria had an increased root surface area compared with axenic plants; the increased area was unlikely to have caused their increased tissue Se accumulation because they did not accumulate more Se when supplied with selenite or selenomethionine. Rhizosphere bacteria also possibly increased plant Se volatilization because they enabled plants to overcome a rate-limiting step in the Se volatilization pathway, i.e. Se accumulation in plant tissues. PMID:9952452

[Distortion and vertical fracture of the root: effect produced by condenser design].

PubMed

Dang, D A; Walton, R E

1990-01-01

The incidence of vertical root fractures and the amount of root distortion created during lateral condensation of gutta-percha with either D11 spreaders or B-finger pluggers were evaluated in vitro. Fifty-five extracted human, single-rooted teeth were instrumented using the step-back flare technique. Ten teeth served as positive controls (obturation to the point of fracture) and five teeth as negative controls (prepared but not obtured). Strain gauges were attached to the root surfaces. In the experimental group, 20 teeth were obturated using a D11 spreader and 20 with a B-finger plugger. Recordings were made of root distortion (expansion) created during obturation. Then, after sectioning the teeth, root surfaces of obturated samples were examined for fractures under the scanning electron microscope. Only the more tapered spreader, the D11, produces vertical root fractures, although very few in number. Also, the D11 spreader caused greater root distortion than did the B-finger plugger.

Thermal measurement of root surface temperatures during application of intracanal laser energy in vitro

NASA Astrophysics Data System (ADS)

Goodis, Harold E.; White, Joel M.; Neev, Joseph

1993-07-01

The use of laser energy to clean, shape, and sterilize a root canal system space involves the generation of heat due to the thermal effect of the laser on the organic tissue contents and dentin walls of that space. If heat generation is above physiologic levels, irreparable damage may occur to the periodontal ligament and surrounding bone. This study measured temperature rise on the outer root surfaces of extracted teeth during intracanal laser exposure. Thirty single rooted, recently extracted teeth free of caries and restorations were accessed pulps extirpated and divided into three groups. Each root canal system was treated with a 1.06 micrometers pulsed Nd:YAG laser with quartz contact probes. Temperatures were recorded for all surfaces (mesial distal, buccal, lingual, apical) with infrared thermography utilizing a detector response time of 1 (mu) sec, sensitivity range (infrared) of 8 to 12 micrometers and a scan rate of 30 frames/sec.

Spread of Mycobacterium avium subsp. paratuberculosis through soil and grass on a mouflon (Ovis aries) pasture.

PubMed

Kaevska, Marija; Lvoncik, S; Lamka, J; Pavlik, I; Slana, I

2014-10-01

The aims of this study were to describe spatial contamination of the environment on a mouflon pasture, as well as to assess the contamination of grass and roots after surface contamination and in depth contamination with feces and buried tissues from animals infected with Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis). Samples of soil, roots, and aerial parts of plants were collected from different locations inside the mouflon pasture, and one control sample site was chosen outside the area where the animals are living. M. a. paratuberculosis DNA was present in all the examined sites and was more often detected in roots than in soil. DNA was detected at up to 80 cm of depth and was spatially more widespread than the initial hypothesis of M. a. paratuberculosis leaching vertically into deeper layers of soil. This study broadens our knowledge of the spread and persistence of M. a. paratuberculosis in an environment with highly infected animals.

In vitro dissolution kinetic study of theophylline from hydrophilic and hydrophobic matrices.

PubMed

Maswadeh, Hamzah M; Semreen, Mohammad H; Abdulhalim, Abdulatif A

2006-01-01

Oral dosage forms containing 300 mg theophylline in matrix type tablets, were prepared by direct compression method using two kinds of matrices, glycerylbehenate (hydrophobic), and (hydroxypropyl)methyl cellulose (hydrophilic). The in vitro release kinetics of these formulations were studied at pH 6.8 using the USP dissolution apparatus with the paddle assemble. The kinetics of the dissolution process were studied by analyzing the dissolution data using four kinetic equations, the zero-order equation, the first-order equation, the Higuchi square root equation and the Hixson-Crowell cube root law. The analysis of the dissolution kinetic data for the theophylline preparations in this study shows that it follows the first order kinetics and the release process involves erosion / diffusion and an alteration in the surface area and diameter of the matrix system, as well as in the diffusion path length from the matrix drug load during the dissolution process. This relation is best described by the use of both the first-order equation and the Hixson-Crowell cube root law.

KSC-2009-2110

NASA Image and Video Library

2009-03-03

CAPE CANAVERAL, Fla. – At NASA's Kennedy Space Center in Florida, a family of wild pigs roots through the grass for food. Overhead, vultures circle, hunting for their share of food. The wild pigs have flourished in the environs around Kennedy, which shares a border with the Merritt Island National Wildlife Refuge, without many predators other than panthers and humans. Pigs were introduced to Florida in the 1500s and are now found statewide in wooded areas close to water. Pigs are omnivores, foraging on the ground and rooting just beneath the surface, which damages the groundcover. Wild pigs eat almost anything that has nutritional value, including tubers, roots, shoots, acorns, fruits, berries, earthworms, amphibians, reptiles and rodents. Their appearance is similar to domestic hogs, but leaner, with a longer, narrower head and a coarser, denser coat. Females may have two litters per year. The piglets are weaned in a few weeks but remain with the mother for several months. Photo credit: NASA/Ben Smegelsky

Herbaceous plant species invading natural areas tend to have stronger adaptive root foraging than other naturalized species

PubMed Central

Keser, Lidewij H.; Visser, Eric J. W.; Dawson, Wayne; Song, Yao-Bin; Yu, Fei-Hai; Fischer, Markus; Dong, Ming; van Kleunen, Mark

2015-01-01

Although plastic root-foraging responses are thought to be adaptive, as they may optimize nutrient capture of plants, this has rarely been tested. We investigated whether nutrient-foraging responses are adaptive, and whether they pre-adapt alien species to become natural-area invaders. We grew 12 pairs of congeneric species (i.e., 24 species) native to Europe in heterogeneous and homogeneous nutrient environments, and compared their foraging responses and performance. One species in each pair is a USA natural-area invader, and the other one is not. Within species, individuals with strong foraging responses, measured as plasticity in root diameter and specific root length, had a higher biomass. Among species, the ones with strong foraging responses, measured as plasticity in root length and root biomass, had a higher biomass. Our results therefore suggest that root foraging is an adaptive trait. Invasive species showed significantly stronger root-foraging responses than non-invasive species when measured as root diameter. Biomass accumulation was decreased in the heterogeneous vs. the homogeneous environment. In aboveground, but not belowground and total biomass, this decrease was smaller in invasive than in non-invasive species. Our results show that strong plastic root-foraging responses are adaptive, and suggest that it might aid in pre-adapting species to becoming natural-area invaders. PMID:25964790

Changes in hydraulic conductance cause the difference in growth response to short-term salt stress between salt-tolerant and -sensitive black gram (Vigna mungo) varieties.

PubMed

Win, Khin Thuzar; Oo, Aung Zaw; Ookawa, Taiichiro; Kanekatsu, Motoki; Hirasawa, Tadashii

2016-04-01

Black gram (Vigna mungo) is an important crop in Asia, However, most black gram varieties are salt-sensitive. The causes of varietal differences in salt-induced growth reduction between two black gram varieties, 'U-Taung-2' (salt-tolerant; BT) and 'Mut Pe Khaing To' (salt-sensitive; BS), were examined the potential for the first step toward the genetic improvement of salt tolerance. Seedlings grown in vermiculite irrigated with full-strength Hoagland solution were treated with 0mM NaCl (control) or 225 mM NaCl for up to 10 days. In the 225 mM NaCl treatment, plant growth rate, net assimilation rate, mean leaf area, leaf water potential, and leaf photosynthesis were reduced more in BS than in BT plants. Leaf water potential was closely related to leaf photosynthesis, net assimilation rate, and increase in leaf area. In response to salinity stress, hydraulic conductance of the root, stem, and petiole decreased more strongly in BS than in BT plants. The reduction in stem and petiole hydraulic conductance was caused by cavitation, whereas the reduction in root hydraulic conductance in BS plants was caused by a reduction in root surface area and hydraulic conductivity. We conclude that the different reduction in hydraulic conductance is a cause of the differences in the growth response between the two black gram varieties under short-term salt stress. Copyright © 2016 Elsevier GmbH. All rights reserved.

Evaluation of LiDAR-Acquired Bathymetric and Topographic Data Accuracy in Various Hydrogeomorphic Settings in the Lower Boise River, Southwestern Idaho, 2007

USGS Publications Warehouse

Skinner, Kenneth D.

2009-01-01

Elevation data in riverine environments can be used in various applications for which different levels of accuracy are required. The Experimental Advanced Airborne Research LiDAR (Light Detection and Ranging) - or EAARL - system was used to obtain topographic and bathymetric data along the lower Boise River, southwestern Idaho, for use in hydraulic and habitat modeling. The EAARL data were post-processed into bare earth and bathymetric raster and point datasets. Concurrently with the EAARL data collection, real-time kinetic global positioning system and total station ground-survey data were collected in three areas within the lower Boise River basin to assess the accuracy of the EAARL elevation data in different hydrogeomorphic settings. The accuracies of the EAARL-derived elevation data, determined in open, flat terrain, to provide an optimal vertical comparison surface, had root mean square errors ranging from 0.082 to 0.138 m. Accuracies for bank, floodplain, and in-stream bathymetric data had root mean square errors ranging from 0.090 to 0.583 m. The greater root mean square errors for the latter data are the result of high levels of turbidity in the downstream ground-survey area, dense tree canopy, and horizontal location discrepancies between the EAARL and ground-survey data in steeply sloping areas such as riverbanks. The EAARL point to ground-survey comparisons produced results similar to those for the EAARL raster to ground-survey comparisons, indicating that the interpolation of the EAARL points to rasters did not introduce significant additional error. The mean percent error for the wetted cross-sectional areas of the two upstream ground-survey areas was 1 percent. The mean percent error increases to -18 percent if the downstream ground-survey area is included, reflecting the influence of turbidity in that area.

Etidronate from Medicine to Endodontics: effects of different irrigation regimes on root dentin roughness

PubMed Central

TARTARI, Talita; DUARTE JUNIOR, Anivaldo Pereira; SILVA JÚNIOR, José Otávio Carrera; KLAUTAU, Eliza Burlamaqui; SILVA E SOUZA JUNIOR, Mario Honorato; SILVA E SOUZA, Patrícia de Almeida Rodrigues

2013-01-01

An increase in dentin roughness, associated with surface composition, contributes to bacterial adherence in recontaminations. Surface roughness is also important for micromechanical interlocking of dental materials to dentin, and understanding the characteristics of the surface is essential to obtain the adhesion of root canal sealers that have different physico-chemical characteristics. Objectives To evaluate the effects of sodium hypochlorite (NaOCl), ethylenediaminetetraacetic (EDTA), etidronic (HEBP), and citric acid (CA) associated with different irrigation regimens on root dentin roughness. Material and Methods Forty-five root halves of anterior teeth were used. The root parts were sectioned in thirds, embedded in acrylic resin and polished to a standard surface roughness. Initially, the samples of each third were randomly assigned into 3 groups and treated as follows: G1 - saline solution (control); G2 - 5% NaOCl+18% HEBP mixed in equal parts; and G3 - 2.5% NaOCl. After initial measuments, the G3 samples were distributed into subgroups G4, G5 and G6, which were subjected to 17% EDTA, 10% CA and 9% HEBP, respectively. Following the new measuments, these groups received a final flush with 2.5% NaOCl, producing G7, G8 and G9. The dentin surface roughness (Ra) was determined before and after treatments using a profilometer. The Wilcoxon test (α<0.05) was used to compare the values before and after treatments, and the Friedman test (α<0.05) to detect any differences among root thirds. Results (i) NaOCl did not affect the surface roughness; (ii) there was a significant increase in roughness after the use of chelating agents (P<0.01); and (iii) only the G3 group showed a difference in surface roughness between apical third and other thirds of the teeth (P<0.0043). Conclusion Only the irrigation regimens that used chelating agents altered the roughness of root dentin. PMID:24212986

Etidronate from medicine to endodontics: effects of different irrigation regimes on root dentin roughness.

PubMed

Tartari, Talita; Duarte Junior, Anivaldo Pereira; Silva Júnior, José Otávio Carrera; Klautau, Eliza Burlamaqui; Silva E Souza Junior, Mario Honorato; Silva E Souza Junior, Patrícia de Almeida Rodrigues

2013-01-01

An increase in dentin roughness, associated with surface composition, contributes to bacterial adherence in recontaminations. Surface roughness is also important for micromechanical interlocking of dental materials to dentin, and understanding the characteristics of the surface is essential to obtain the adhesion of root canal sealers that have different physico-chemical characteristics. To evaluate the effects of sodium hypochlorite (NaOCl), ethylenediaminetetraacetic (EDTA), etidronic (HEBP), and citric acid (CA) associated with different irrigation regimens on root dentin roughness. Forty-five root halves of anterior teeth were used. The root parts were sectioned in thirds, embedded in acrylic resin and polished to a standard surface roughness. Initially, the samples of each third were randomly assigned into 3 groups and treated as follows: G1 - saline solution (control); G2 - 5% NaOCl+18% HEBP mixed in equal parts; and G3 - 2.5% NaOCl. After initial measuments, the G3 samples were distributed into subgroups G4, G5 and G6, which were subjected to 17% EDTA, 10% CA and 9% HEBP, respectively. Following the new measuments, these groups received a final flush with 2.5% NaOCl, producing G7, G8 and G9. The dentin surface roughness (Ra) was determined before and after treatments using a profilometer. The Wilcoxon test (α<0.05) was used to compare the values before and after treatments, and the Friedman test (α<0.05) to detect any differences among root thirds. (i) NaOCl did not affect the surface roughness; (ii) there was a significant increase in roughness after the use of chelating agents (P<0.01); and (iii) only the G3 group showed a difference in surface roughness between apical third and other thirds of the teeth (P<0.0043). Only the irrigation regimens that used chelating agents altered the roughness of root dentin.

The role of root distribution in eco-hydrological modeling in semi-arid regions

NASA Astrophysics Data System (ADS)

Sivandran, G.; Bras, R. L.

2010-12-01

In semi arid regions, the rooting strategies employed by vegetation can be critical to its survival. Arid regions are characterized by high variability in the arrival of rainfall, and species found in these areas have adapted mechanisms to ensure the capture of this scarce resource. Niche separation, through rooting strategies, is one manner in which different species coexist. At present, land surface models prescribe rooting profiles as a function of only the plant functional type of interest with no consideration for the soil texture or rainfall regime of the region being modeled. These models do not incorporate the ability of vegetation to dynamically alter their rooting strategies in response to transient changes in environmental forcings and therefore tend to underestimate the resilience of many of these ecosystems. A coupled, dynamic vegetation and hydrologic model, tRIBS+VEGGIE, was used to explore the role of vertical root distribution on hydrologic fluxes. Point scale simulations were carried out using two vertical root distribution schemes: (i) Static - a temporally invariant root distribution; and (ii) Dynamic - a temporally variable allocation of assimilated carbon at any depth within the root zone in order to minimize the soil moisture-induced stress on the vegetation. The simulations were forced with a stochastic climate generator calibrated to weather stations and rain gauges in the semi-arid Walnut Gulch Experimental Watershed in Arizona. For the static root distribution scheme, a series of simulations were carried out varying the shape of the rooting profile. The optimal distribution for the simulation was defined as the root distribution with the maximum mean transpiration over a 200 year period. This optimal distribution was determined for 5 soil textures and using 2 plant functional types, and the results varied from case to case. The dynamic rooting simulations allow vegetation the freedom to adjust the allocation of assimilated carbon to different rooting depths in response to changes in stress caused by the redistribution and uptake of soil moisture. The results obtained from these experiments elucidate the strong link between plant functional type, soil texture and climate and highlight the potential errors in the modeling of hydrologic fluxes from imposing a static root profile.

CASIROZ: Root parameters and types of ectomycorrhiza of young beech plants exposed to different ozone and light regimes.

PubMed

Zeleznik, P; Hrenko, M; Then, C; Koch, N; Grebenc, T; Levanic, T; Kraigher, H

2007-03-01

Tropospheric ozone (O(3)) triggers physiological changes in leaves that affect carbon source strength leading to decreased carbon allocation below-ground, thus affecting roots and root symbionts. The effects of O(3) depend on the maturity-related physiological state of the plant, therefore adult and young forest trees might react differently. To test the applicability of young beech plants for studying the effects of O(3) on forest trees and forest stands, beech seedlings were planted in containers and exposed for two years in the Kranzberg forest FACOS experiment (Free-Air Canopy O(3) Exposure System, http://www.casiroz.de ) to enhanced ozone concentration regime (ambient [control] and double ambient concentration, not exceeding 150 ppb) under different light conditions (sun and shade). After two growing seasons the biomass of the above- and below-ground parts, beech roots (using WinRhizo programme), anatomical and molecular (ITS-RFLP and sequencing) identification of ectomycorrhizal types and nutrient concentrations were assessed. The mycorrhization of beech seedlings was very low ( CA. 5 % in shade, 10 % in sun-grown plants), no trends were observed in mycorrhization (%) due to ozone treatment. The number of Cenococcum geophilum type of ectomycorrhiza, as an indicator of stress in the forest stands, was not significantly different under different ozone treatments. It was predominantly occurring in sun-exposed plants, while its majority share was replaced by Genea hispidula in shade-grown plants. Different light regimes significantly influenced all parameters except shoot/root ratio and number of ectomycorrhizal types. In the ozone fumigated plants the number of types, number of root tips per length of 1 to 2 mm root diameter, root length density per volume of soil and concentration of Mg were significantly lower than in control plants. Trends to a decrease were found in root, shoot, leaf, and total dry weights, total number of root tips, number of vital mycorrhizal root tips, fine root (mass) density, root tip density per surface, root area index, concentration of Zn, and Ca/Al ratio. Due to the general reduction in root growth indices and nutrient cycling in ozone-fumigated plants, alterations in soil carbon pools could be predicted.

Earth observations taken during STS-41D mission.

NASA Image and Video Library

2009-06-25

41D-42-023 (30 Aug-5 Sept 1984) --- The Gascoyne River, an intermittent large wash 500 miles north of Perth, Australia, which flows for nearly a thousand miles across the Macadam Plains. The scant water available in this area either is absorbed in the sands and gravel of the watercourse or quickly evaporates. Most water flows beneath the surface, then, giving support to plant root systems and sustaining green vegetation along the watercourses.

Effect of differently pelletized digestate on the plant growth of spring wheat

NASA Astrophysics Data System (ADS)

Dietrich, Nils; Knoop, Christine; Raab, Thomas

2017-04-01

In Germany, biowaste is used in more than 100 biogas plants and has increasing potential as a fermentation substrate. To optimise waste cycle management organic digestates should be redistributed and innovative products for soil amendment of agricultural areas could be developed. The BMBF-funded VeNGA project seeks to find answers on how to improve the properties of soil amendments produced from fermentation residues. Here, we report findings from our study that focuses on plant growth and soil development. Within a three-month rhizotron experiment, the influence of differently prepared fermentation residues on the root development of summer wheat was investigated. The four variants of the prepared digestate (rolled pellet, pressed pellet, shredded, loose) were tested under constant conditions in the greenhouse on two soils with different textures (sandy and loamy-sand). All fermentation residues originated from the same batch and were composted before the preparation to ensure adequate hygienisation. Depending on preparation type and soil substrate significant differences in root growth and root development have been observed. Plant growth was most intense in the rhizotron experiment with the loose digestate, indicating high nutrient availability due to the large surface area of the organic matter. Plant growth in the substrate with the rolled and pressed pellets was less pronounced, indicating a more persistent stability of the pellets. In rhizotrons applied with rolled and pressed pellets root growth into the mineral fabric was significantly lower in sandy substrate than in the loamy-sand. However, in the sandy substrate root growth within the rolled pellets was more intense than in the substrate with the pressed pellets. Obviously, the different production techniques of the pellets seem to have an influence on the rooting of the pellets and facilitate the long term stability of soil organic carbon. Furthermore, the comparison of the two different textures indicate, that sandy substrates benefit more from the positive effects of soil amendments on increased water retention than loamy substrates.

Fate and degradation of petroleum hydrocarbons in stormwater bioretention cells

NASA Astrophysics Data System (ADS)

LeFevre, Gregory Hallett

This dissertation describes the investigation of the fate of hydrocarbons in stormwater bioretention areas and those mechanisms that affect hydrocarbon fate in such systems. Seventy-five samples from 58 bioretention areas were collected and analyzed to measure total petroleum hydrocarbon (TPH) residual and biodegradation functional genes. TPH residual in bioretention areas was greater than background sites but low overall (<3 µg/kg), and well below either the TPH concentration of concern or the expected concentration, assuming no losses. Bioretention areas with deep-root vegetation contained significantly greater quantites of bacterial 16S rRNA genes and two functional genes involved in hydrocarbon biodegradation. Field soils were capable of mineralizing naphthalene, a polycyclic aromatic hydrocarbon (PAH) when incubated in the laboratory. In an additional laboratory investigation, a column study was initiated to comprehensively determine naphthalene fate in a simulated bioretention cell using a 14C-labeled tracer. Sorption to soil was the greatest sink of naphthalene in the columns, although biodegradation and vegetative uptake were also important loss mechanisms. Little leaching occurred following the first flush, and volatilization was insignificant. Significant enrichment of naphthalene degrading bacteria occurred over the course of the experiment as a result of naphthalene exposure. This was evident from enhanced naphthalene biodegradation kinetics (measured via batch tests), significant increases in naphthalene dioxygenase gene quantities, and a significant correlation observed between naphthalene residual and biodegradation functional genes. Vegetated columns outperformed the unplanted control column in terms of total naphthalene removal and biodegradation kinetics. As a result of these experiments, a final study focused on why planted systems outperform unplanted systems was conducted. Plant root exudates were harvested from hydroponic setups for three types of plants. Additionally, a solution of artificial root exudates (AREs) as prepared. Exudates were digested using soil bacteria to create metabolized exudates. Raw and metabolized exudates were characterized for dissolved organic carbon, specific UV absorbance, spectral slope, florescence index, excitation-emission matrices, and surface tension. Significant differences on character were observed between the harvested exudates and the AREs, as well as between the raw and metabolized exudates. Naphthalene desorption from an aged soil was enhanced in the presence of raw exudates. The surface tension in samples containing raw harvested exudates was reduced compared to samples containing the metabolized exudates. Plant root exudates may therefore facilitate phytoremediation by enhancing contaminant desorption and improving bioavailability. Overall, this research concludes that heavily planted bioretention systems are a sustainable solution to mitigating stormwater hydrocarbon pollution as a result of likely enhanced contaminant desorption, and improved biodegradation and plant uptake in such systems.

Roughness of biopores and cracks in Bt-horizons by confocal laser scanning microscopy

NASA Astrophysics Data System (ADS)

Leue, Martin; Gerke, Horst H.

2016-04-01

During preferential flow events in structured soils, the movement of water and reactive solutes is mostly restricted to larger inter-aggregate pores, cracks, and biopores. The micro-topography of such macropores in terms of pore shapes, geometry, and roughness is crucial for describing the exchange of water and solutes between macropores and the soil matrix. The objective of this study was to determine the surface roughness of intact structural surfaces from the Bt-horizon of Luvisols by confocal laser scanning microscopy. For this purpose, samples with the structural surface types including cracks with and without clay-organic coatings from Bt-horizons developed on loess and glacial till were compared. The surface roughness of these structures was calculated in terms of three parameters from selected surface regions of 0.36 mm² determined with a confocal laser scanning microscope of the type Keyence VK-X100K. These data were evaluated in terms of the root-mean-squared roughness, Rq, the curvature, Rku, and the ratio between surface area and base area, RA. Values of Rq and RA were smaller for coated as compared to uncoated cracks and earthworm burrows of the Bt-horizons from both parent materials. The results indicated that the illuviation of clayey material led to a "smoothing" of the crack surfaces, which was similar for the coarser textured till-Bt and the finer-textured loess-Bt surfaces. The roughness indicated by Rq and RA values was only slightly smaller and that indicated by Rku slightly higher for the structural surfaces from the loess as compared to those from the glacial till. These results suggest a minor importance of the parent material on the roughness of structural surfaces in the Bt-horizon. The similarity of Rq, RA, and Rku values between surfaces of earthworm burrows and uncoated cracks did not confirm an expected smoothing effect of the burrow walls by the earthworm. In contrast to burrow walls, root channels from the loess-Bt were smoother than the surfaces of the other structure types, suggesting that the two types of biopores have to be distinguished when describing preferential flow and macropore-matrix exchange. Nevertheless, the confocal laser microscopy technique proved useful for characterizing the roughness of intact structural surfaces.

In situ investigation of the mechanisms of the transport to tissues of polycyclic aromatic hydrocarbons adsorbed onto the root surface of Kandelia obovata seedlings.

PubMed

Li, Ruilong; Zhu, Yaxian; Zhang, Yong

2015-06-01

A novel method for in situ determination of the polycyclic aromatic hydrocarbons (PAHs) adsorbed onto the root surface of Kandelia obovata seedlings was established using laser-induced time-resolved nanosecond fluorescence spectroscopy (LITRF). The linear dynamic ranges for the established method were 1.5-1240ng/spot for phenanthrene, 1.0-1360ng/spot for pyrene and 5.0-1220ng/spot for benzo[a]pyrene. Then, the mechanisms of PAHs transport from the Ko root surface to tissues were investigated. The three-phase model including fast, slow and very slow fractions was superior to the single or dual-phase model to describe the PAHs transport processes. Moreover, the fast fraction of PAHs transport process was mainly due to passive movement, while the slow and very slow fractions were not. Passive movement was the main process of B[a]P adsorbed onto Ko root surface transport to tissues. In addition, the extent of the PAHs transport to Ko root tissues at different salinity were evaluated. Copyright © 2015 Elsevier Ltd. All rights reserved.

Molecular Genetics of Root Thigmoresponsiveness in Arabidopsis thaliana

NASA Technical Reports Server (NTRS)

Masson, Patrick H.

2002-01-01

The molecular mechanisms that allow plant roots to use gravity and touch as growth guides are investigated. We are using a molecular genetic strategy in Arabidopsis thaliana to study these processes. When Arabidopsis thaliana seedlings grow on tilted hard-agar surfaces, their roots develop a wavy pattern of growth which appears to derive from a succession of left-handed and right-handed circumnutation-like processes triggered by gravity and touch stimulation (Okada and Shimura, 1990; Rutherford et al., 1998; Rutherford and Masson, 1996). Interestingly, mutations that affect root waving on tilted hard-agar surfaces can be identified and characterized. Some of these mutations affect root gravitropism, while others appear to be responsible for the production of abnormal waves (no waves, compressed or square waves, coils) without affecting gravitropism. The specific objectives of this project were to functionally characterize two genes (WVD2 and WVD6) which are required for root waving on tilted agar surfaces, but not for root gravitropism. Specific objectives included a physiological and cytological analysis of the mutants, and molecular cloning and characterization of the corresponding genes. As summarized in this paper, we have reached these objectives. We have also identified and partially characterized other mutations that affect root skewing on hard-agar surfaces (sku5-1 and ago1), and have completed our work on the root-wave phenotype associated with mutations in genes of the tryptophan biosynthesis pathway (Lynn et al., 1999; Rutherford et al., 1998; Sedbrook et al., 2000, 2002). We briefly describe our progress on the cloning and characterization of WVD6, WVD2 and SKU5, and provide a list of papers (published, or in preparation) that derived from this grant. We also discuss the biological implications of our findings, with special emphasis on the analysis of WVD2.

The effect of iron plaque on uptake and translocation of norfloxacin in rice seedlings grown in paddy soil.

PubMed

Yan, Dafang; Ma, Wei; Song, Xiaojing; Bao, Yanyu

2017-03-01

Although the role of iron plaque on rice root surface has been investigated in recent years, its effect on antibiotic uptake remains uncertain. In the study, pot experiment was conducted to investigate the effect of iron plaque on uptake and translocation of norfloxacin (adding 10 and 50 mg·kg -1 treatments) in rice seedlings grown in paddy soil. Iron plaque was induced by adding different amounts of Fe(II) in soil. The results showed that the presence of norfloxacin can decrease the amount of iron plaque induced. After rice with iron plaque induced, norfloxacin was mainly accumulated in iron plaque on root surface, followed by inside root, but its translocation from root to other rice tissues is not observed. Iron plaque played the role of a barrier for norfloxacin uptake into rice roots under high norfloxacin concentration of 50 mg·kg -1 , however not that under low concentration of 10 mg·kg -1 . And the barrier function was the most strongest with adding Fe(II) of 30 mg·kg -1 as combined action of iron plaque and rhizosphere effect. Fluorescence microscope analysis showed that norfloxacin mainly distributed in the outside of root cell, which showed its translocation as apoplastic pathway in rice. Comparing with non-rhizosphere, more norfloxacin was accumulated in rhizosphere soil. Maybe, strong root oxidization (high Eh values) induced more iron oxide formation in rhizosphere and on root surface, which led to norfloxacin's mobility towards to rhizosphere through its strong adsorption of iron oxides and then promoted its uptake by rice on root surface.

Imaging tree roots with borehole radar

Treesearch

John R. Butnor; Kurt H. Johnsen; Per Wikstrom; Tomas Lundmark; Sune Linder

2006-01-01

Ground-penetrating radar has been used to de-tect and map tree roots using surface-based antennas in reflection mode. On amenable soils these methods can accurately detect lateral tree roots. In some tree species (e.g. Pinus taeda, Pinus palustris), vertically orientated tap roots directly beneath the tree, comprise most of the root mass. It is...

The Molecular Mechanism of Ethylene-Mediated Root Hair Development Induced by Phosphate Starvation

PubMed Central

Song, Li; Yu, Haopeng; Dong, Jinsong; Liu, Dong

2016-01-01

Enhanced root hair production, which increases the root surface area for nutrient uptake, is a typical adaptive response of plants to phosphate (Pi) starvation. Although previous studies have shown that ethylene plays an important role in root hair development induced by Pi starvation, the underlying molecular mechanism is not understood. In this work, we characterized an Arabidopsis mutant, hps5, that displays constitutive ethylene responses and increased sensitivity to Pi starvation due to a mutation in the ethylene receptor ERS1. hps5 accumulates high levels of EIN3 protein, a key transcription factor involved in the ethylene signaling pathway, under both Pi sufficiency and deficiency. Pi starvation also increases the accumulation of EIN3 protein. Combined molecular, genetic, and genomic analyses identified a group of genes that affect root hair development by regulating cell wall modifications. The expression of these genes is induced by Pi starvation and is enhanced in the EIN3-overexpressing line. In contrast, the induction of these genes by Pi starvation is suppressed in ein3 and ein3eil1 mutants. EIN3 protein can directly bind to the promoter of these genes, some of which are also the immediate targets of RSL4, a key transcription factor that regulates root hair development. Based on these results, we propose that under normal growth conditions, the level of ethylene is low in root cells; a group of key transcription factors, including RSL4 and its homologs, trigger the transcription of their target genes to promote root hair development; Pi starvation increases the levels of the protein EIN3, which directly binds to the promoters of the genes targeted by RSL4 and its homologs and further increase their transcription, resulting in the enhanced production of root hairs. This model not only explains how ethylene mediates root hair responses to Pi starvation, but may provide a general mechanism for how ethylene regulates root hair development under both stress and non-stress conditions. PMID:27427911

Smear layer production by 3 rotary reamers with different cutting blade designs in straight root canals: a scanning electron microscopic study.

PubMed

Jeon, In-Soo; Spångberg, Larz S W; Yoon, Tai-Cheol; Kazemi, Reza B; Kum, Kee-Yeon

2003-11-01

The design of the cutting blade of rotary instruments may affect the outcome of root canal instrumentation in terms of cleanliness. The aim of this scanning electron microscopic study was to compare the quality and amount of smear layer generated in the apical third of straight root canals by 2 rotary nickel-titanium reamers and 1 rotary steel reamer with different cutting blade designs. Seventy intact, single-rooted human mandibular premolars with straight, fully developed roots were selected for this study. Before instrumentation, the cervical portion of all teeth was removed by using a microtome (Isomet), leaving 13-mm-long roots. Automated preparation was performed with ProFile (n = 20) and Hero 642 (n = 20) reamers by using the crown-down technique and with a stainless steel engine reamer (Mani; n = 20) by using a reaming motion. All root canals were instrumented to No. 40. A control group (pulp extirpation with barbed broaches; n = 10) was also included. Irrigation with 3 mL of a 1% sodium hypochlorite (NaOCl) solution was performed after each instrumentation. After the instrumentation, each root was split longitudinally, and a scanning electron microscope was used to examine the selected areas of the canal walls at the apical third from 2 different perspectives. A 4-category scoring system for smear layer was used, and the resulting scores were statistically analyzed. The least smear layer remained in the Hero 642 group at the selected apical third of straight root canals (P < .05). However, all instruments left a smear layer. The surface texture of the smear layer, in addition to the depth and the frequency of packed materials into the dentinal tubules, varied with instrument type. These data revealed that the design of the cutting blade of rotary instruments can affect root canal cleanliness in straight root canals. This information may be useful in the selection of nickel-titanium rotary reamers.

Molecular and Morpho-Agronomical Characterization of Root Architecture at Seedling and Reproductive Stages for Drought Tolerance in Wheat

PubMed Central

Vinod; Naik, Bhojaraja K.; Chand, Suresh; Deshmukh, Rupesh; Mallick, Niharika; Singh, Sanjay; Singh, Nagendra Kumar; Tomar, S. M. S.

2016-01-01

Water availability is a major limiting factor for wheat (Triticum aestivum L.) production in rain-fed agricultural systems worldwide. Root architecture is important for water and nutrition acquisition for all crops, including wheat. A set of 158 diverse wheat genotypes of Australian (72) and Indian (86) origin were studied for morpho-agronomical traits in field under irrigated and drought stress conditions during 2010–11 and 2011-12.Out of these 31 Indian wheat genotypes comprising 28 hexaploid (Triticum aestivum L.) and 3 tetraploid (T. durum) were characterized for root traits at reproductive stage in polyvinyl chloride (PVC) pipes. Roots of drought tolerant genotypes grew upto137cm (C306) as compared to sensitive one of 63cm with a mean value of 94.8cm. Root architecture traits of four drought tolerant (C306, HW2004, HD2888 and NI5439) and drought sensitive (HD2877, HD2012, HD2851 and MACS2496) genotypes were also observed at 6 and 9 days old seedling stage. The genotypes did not show any significant variation for root traits except for longer coleoptiles and shoot and higher absorptive surface area in drought tolerant genotypes. The visible evaluation of root images using WinRhizo Tron root scanner of drought tolerant genotype HW2004 indicated compact root system with longer depth while drought sensitive genotype HD2877 exhibited higher horizontal root spread and less depth at reproductive stage. Thirty SSR markers were used to study genetic variation which ranged from 0.12 to 0.77 with an average value of 0.57. The genotypes were categorized into three subgroups as highly tolerant, sensitive, moderately sensitive and tolerant as intermediate group based on UPGMA cluster, STRUCTURE and principal coordinate analyses. The genotypic clustering was positively correlated to grouping based on root and morpho-agronomical traits. The genetic variability identified in current study demonstrated these traits can be used to improve drought tolerance and association mapping. PMID:27280445

Use of different surface covering materials to enhance removal of radiocaesium in plants and upper soil from orchards in Fukushima prefecture.

PubMed

Sato, Mamoru; Akai, Hiroko; Saito, Yuichi; Takase, Tsugiko; Kikunaga, Hidetoshi; Sekiya, Nobuhito; Ohtsuki, Tsutomu; Yamaguchi, Katsuhiko

2017-04-04

The effectiveness of a decontamination methodology whereby herbaceous plants were grown through different materials covering the soil surface followed by subsequent removal of the material, associated plant tissues and attached soil on 137 Cs removal from soil was evaluated. Revegetation netting sown with Kentucky bluegrass and white clover had a high effectiveness in 137 Cs removal when rolling up the plants, roots, and rhizosphere soil approximately 6 months after sowing. The removal rate was lower when there was higher 137 Cs vertical migration down the soil profile. The maximum removal effectiveness of 93.1% was observed by rolling up fertilized Kentucky bluegrass with a well-developed root mat without netting, indicating that applying nutrients to encourage the development of roots or root mats in the 3 cm topsoil rhizosphere is an efficient technology to increase the decontamination effect of plant removal in orchards. Netting and weeding were able to remove up to 80% of 137 Cs in the soil without the use of heavy machinery. There was a significant relationship between the removal ratio and the removed soil weight per area. Using the relationship on the site below the canopy, removal of 14.3 kg m -2 DW soil would achieve a removal ratio of 80%. The effectiveness of the technique will decrease with time as radiocaesium migrates down the soil profile but this would be expected to occur slowly in many soils. Copyright © 2017 Elsevier Ltd. All rights reserved.

Hydraulic Redistribution: A Modeling Perspective

NASA Astrophysics Data System (ADS)

Daly, E.; Verma, P.; Loheide, S. P., III

2014-12-01

Roots play a key role in the soil water balance. They extract and transport water for transpiration, which usually represents the most important soil water loss in vegetated areas, and can redistribute soil water, thereby increasing transpiration rates and enhancing root nutrient uptake. We present here a two-dimensional model capable of describing two key aspects of root water uptake: root water compensation and hydraulic redistribution. Root water compensation is the ability of root systems to respond to the reduction of water uptake from areas of the soil with low soil water potential by increasing the water uptake from the roots in soil parts with higher water potential. Hydraulic redistribution is a passive transfer of water through the root system from areas of the soil with greater water potential to areas with lower water potential. Both mechanisms are driven by gradients of water potential in the soil and the roots. The inclusion of root water compensation and hydraulic redistribution in models can be achieved by describing root water uptake as a function of the difference in water potential between soil and root xylem. We use a model comprising the Richards equation for the water flow in variably saturated soils and the Darcy's equation for the water flow in the xylem. The two equations are coupled via a sink term, which is assumed to be proportional to the difference between soil and xylem water potentials. The model is applied in two case studies to describe vertical and horizontal hydraulic redistribution and the interaction between vegetation with different root depths. In the case of horizontal redistribution, the model is used to reproduce the fluxes of water across the root system of a tree subjected to uneven irrigation. This example can be extended to situations when only part of the root system has access to water, such as vegetation near creeks, trees at the edge of forests, and street trees in urban areas. The second case is inspired by recent agro-ecosystems experiments that combined different vegetation species to increase crop yield. The presence of deep rooted plants (nursing species) near shallow rooted crops (nursed species) enhanced crop growth thanks to vertical and horizontal hydraulic redistribution. The model is able to reproduce the patterns of water redistribution observed in this scenario.

Analysis of growth patterns during gravitropic curvature in roots of Zea mays by use of a computer-based video digitizer

NASA Technical Reports Server (NTRS)

Nelson, A. J.; Evans, M. L.

1986-01-01

A computer-based video digitizer system is described which allows automated tracking of markers placed on a plant surface. The system uses customized software to calculate relative growth rates at selected positions along the plant surface and to determine rates of gravitropic curvature based on the changing pattern of distribution of the surface markers. The system was used to study the time course of gravitropic curvature and changes in relative growth rate along the upper and lower surface of horizontally-oriented roots of maize (Zea mays L.). The growing region of the root was found to extend from about 1 mm behind the tip to approximately 6 mm behind the tip. In vertically-oriented roots the relative growth rate was maximal at about 2.5 mm behind the tip and declined smoothly on either side of the maximum. Curvature was initiated approximately 30 min after horizontal orientation with maximal (50 degrees) curvature being attained in 3 h. Analysis of surface extension patterns during the response indicated that curvature results from a reduction in growth rate along both the upper and lower surfaces with stronger reduction along the lower surface.

Influence of ethanol on dentin roughness, surface free energy, and interaction between AH Plus and root dentin.

PubMed

Pantoja, Carlos Augusto de Morais Souto; Silva, Diogo Henrique da; Soares, Adriana de Jesus; Ferraz, Caio Cezar Randi; Gomes, Brenda Paula Figueiredo de Almeida; Zaia, Alexandre Augusto; Almeida, José Flávio Affonso de

2018-01-01

This study aimed to evaluate the influence of different ethanol concentrations on dentin roughness, surface free energy, and contact angle between AH Plus and the root canal dentin. One hundred human maxillary anterior teeth were split longitudinally and 200 dentin specimens were polished to make the surface flatter and smoother. An acrylic bar was positioned between two dentin specimens and impression material was added to create a block, simulating an instrumented root canal space. Specimens were removed from the mold and cleaned in an ultrasonic bath for 10 min. Thereafter, dentin specimens were divided into four groups (n = 50) according to the drying methods used: a) wet: vacuum only, b) paper points: vacuum + absorbent paper points, c) 70% alcohol: 70% alcohol (1 min) + vacuum + absorbent paper points, and d) 100% alcohol: 100% alcohol (1 min) + vacuum + absorbent paper points. A rugosimeter and a goniometer were used to verify the roughness (Ra) and to measure the surface free energy and the contact angle between the AH Plus sealer and the root canal dentin. ANOVA and Tukey tests (α = 0.05) were used for statistical analysis. The 70% and 100% ethanol groups showed significantly decreased roughness as well as increased surface free energy in the root canal dentin when compared to the wet and paper point groups. In addition, ethanol significantly reduced the contact angle between the AH Plus sealer and the root canal dentin. Ethanol solutions (70% and 100%) provide better wettability of AH Plus sealer on dentin surfaces.

Community structure of fish larvae in mangroves with different root types in Labuhan coastal area, Sepulu - Madura

NASA Astrophysics Data System (ADS)

Muzaki, Farid Kamal; Giffari, Aninditha; Saptarini, Dian

2017-06-01

Mangrove root complexity and shading are well known to give positive correlation for both juveniles and adult fishes. However, it is remain unclear whether that complexity would affect the community of fish larvae (ichthyoplankton). This study aimed to address the question, especially in mangrove area in coastal area of Sepulu, Madura which projected as a mangrove protection area. Sampling periods were from March to May, 2016. The samples of fish larvae were collected by plankton net (mesh-size 0.150 and 0.265 mm) from six different locations representing different root types (stilt root, pneumatophore, combination of stilt root-pneumatophore and unvegetated area). As the results, 6 families were identified, namely Gobiidae, Blennidae, Pomacentridae, Carangidae, Engraulidae and Ambassidae, respectively. Gobiidae seems to be the most abundant and widely dispersed in the area. Results of two-way AnovadanTukey HSD (both at p=0.05) indicate that there were significant difference in the larval abundance regarding locations, sampling periods and interaction of both factors. As for number of taxa, significant difference occurred only from factors of locations and sampling periods, but not for interaction of both factors. Highest larval abundance and number of taxa occurred in Rhizophoraspp (with stilt root), indicating that root complexity would affect the community of fish larvae. Ordination by canonical analysis shows that different taxa of the fish larvae are tend to be distributed on different locations.

PIV measurements and flow characteristics downstream of mangrove root models

NASA Astrophysics Data System (ADS)

Kazemi, Amirkhosro; Curet, Oscar

2016-11-01

Mangrove forests attracted attentions as a solution to protect coastal areas exposed to sea-level rising, frequent storms, and tsunamis. Mangrove forests found in tide-dominated flow regions are characterized by their massive and complex root systems, which play a prominent role in the structure of tidal flow currents. To understand the role of mangrove roots in flow structure, we modeled mangrove roots with rigid and flexible arrays of cylinders with different spacing between them as well as different configurations. In this work, we investigate the fluid dynamics downstream of the models using a 2-D time-resolved particle image velocimetry (PIV) and flow visualization. We carried out experiments for four different Reynolds number based on cylinder diameters ranges from 2200 to 12000. We present time-averaged and time-resolved flow parameters including velocity distribution, vorticity, streamline, Reynolds shear stress and turbulent kinetic energy. The results show that the flow structure has different vortex shedding downstream of the cylinders due to interactions of shear layers separating from cylinders surface. The spectral analysis of the measured velocity data is also performed to obtain Strouhal number of the unsteady flow in the cylinder wake.

Effect of simulated climate warming on the morphological and physiological traits of Elsholtzia haichowensis in copper contaminated soil.

PubMed

Guan, Ming; Jin, Zexin; Li, Junmin; Pan, Xiaocui; Wang, Suizi; Li, Yuelin

2016-01-01

The aim of this study was to investigate the effects of temperature and Cu on the morphological and physiological traits of Elsholtzia haichowensis grown in soils amended with four Cu concentrations (0, 50, 500, and 1000 mg kg(-1)) under ambient temperature and slight warming. At the same Cu concentration, the height, shoot dry weight, total plant dry weight, and root morphological parameters such as length, surface area and tip number of E. haichowensis increased due to the slight warming. The net photosynthetic rate, stomatal conductance, transpiration, light use efficiency were also higher under the slight warming than under ambient temperature. The increased Cu concentrations, total Cu uptake, bioaccumulation factors and tolerance indexes of shoots and roots were also observed at the slight warming. The shoot dry weight, root dry weight, total plant dry weight and the bioaccumulation factors of shoots and roots at 50 mg Cu kg(-1) were significantly higher than those at 500 and 1000 mg Cu kg(-1) under the slight warming. Therefore, the climate warming may improve the ability of E. haichowensis to phytoremediate Cu-contaminated soil, and the ability improvement greatly depended on the Cu concentrations in soils.

Armeria maritima from a calamine heap--initial studies on physiologic-metabolic adaptations to metal-enriched soil.

PubMed

Olko, A; Abratowska, A; Zyłkowska, J; Wierzbicka, M; Tukiendorf, A

2008-02-01

Plants of Armeria maritima are found both on unpolluted sites and on soils strongly polluted with heavy metals. Seedlings of A. maritima from a zinc-lead calamine heap in ore-mining region (Bolesław population) and from unpolluted area (Manasterz population) were tested to determine the zinc, cadmium and lead tolerance. In hydroponic experiments Bolesław population was more tolerant to zinc, cadmium and lead. Localization of heavy metals in roots was determined using the histochemical method for detecting metal-complexes with dithizone. Their accumulation was found in root hairs, rhizoderma and at the surface of the central cylinder. Glutathione level in plants increased after metal treatment of both populations. However, its high level was not correlated with phytochelatin production. These metal-binding complexes were not detected in plants exposed to zinc, cadmium or lead. Changes of organic acids concentrations in Armeria treated with metals may suggest their role in metal translocation from roots to shoots. The content of organic acids, especially malate, decreased in the roots and increased in the leaves. These changes may be important in Pb-tolerance of Manasterz population and in Zn-, Cd-tolerance of calamine population from Bolesław.

Fine Root Abundance and Dynamics of Stone Pine (Pinus cembra) at the Alpine Treeline Is Not Impaired by Self-shading

PubMed Central

Kubisch, Petra; Leuschner, Christoph; Coners, Heinz; Gruber, Andreas; Hertel, Dietrich

2017-01-01

Low temperatures are crucial for the formation of the alpine treeline worldwide. Since soil temperature in the shade of tree canopies is lower than in open sites, it was assumed that self-shading may impair the trees’ root growth performance. While experiments with tree saplings demonstrate root growth impairment at soil temperatures below 5–7°C, field studies exploring the soil temperature – root growth relationship at the treeline are missing. We recorded soil temperature and fine root abundance and dynamics in shaded and sun-exposed areas under canopies of isolated Pinus cembra trees at the alpine treeline. In contrast to the mentioned assumption, we found more fine root biomass and higher fine root growth in colder than in warmer soil areas. Moreover, colder areas showed higher fine root turnover and thus lower root lifespan than warmer places. We conclude that P. cembra balances enhanced fine root mortality in cold soils with higher fine root activity and by maintaining higher fine root biomass, most likely as a response to shortage in soil resource supply. The results from our study highlight the importance of in situ measurements on mature trees to understand the fine root response and carbon allocation pattern to the thermal growth conditions at the alpine treeline. PMID:28469633

Influence of Root Exudates and Soil on Attachment of Pasteuria penetrans to Meloidogyne arenaria

PubMed Central

Liu, Chang; Ji, Pingsheng; Mekete, Tesfamariam; Joseph, Soumi

2017-01-01

The bacterium Pasteuria penetrans is a parasite of root-knot nematodes (Meloidogyne spp.). Endospores of P. penetrans attach to the cuticle of second-stage juveniles (J2) and subsequently sterilize infected females. When encumbered by large numbers of spores, juveniles are less mobile and their ability to infect roots is reduced. This study looked at different factors that influence spore attachment of P. penetrans to the root-knot nematode Meloidogyne arenaria. Pretreatment of J2 with root exudates of eggplant (Solanum melongena cv. Black beauty) reduced spore attachment compared with pretreatment with phosphate-buffered saline (PBS), suggesting that the nematode surface coat was altered or the spore recognition domains on the nematode surface were blocked. Spore attachment was equally reduced following exposure to root exudates from both host and nonhost plants for M. arenaria, indicating a common signal that affects spore attachment. Although phytohormones have been shown to influence the lipophilicity of the nematode surface coat, auxins and kinetins did not affect spore attachment compared with PBS. Root exudates reduced spore attachment more in sterilized soil than in natural soil. Sterilization may have eliminated microbes that consume root exudates, or altered the chemical components of the soil solution or root exudates. Root exudates caused a greater decrease in spore attachment in loamy sand than in a sandy loam soil. The sandy loam had higher clay content than the loamy sand, which may have resulted in more adsorption of compounds in the root exudates that affect spore attachment. The components of the root exudates could have also been modified by soil type. The results of this study demonstrate that root exudates can decrease the attachment of P. penetrans endospores to root-knot nematodes, indicating that when these nematodes enter the root zone their susceptibility to spore attachment may decrease. PMID:29062153

Influence of Root Exudates and Soil on Attachment of Pasteuria penetrans to Meloidogyne arenaria.

PubMed

Liu, Chang; Timper, Patricia; Ji, Pingsheng; Mekete, Tesfamariam; Joseph, Soumi

2017-09-01

The bacterium Pasteuria penetrans is a parasite of root-knot nematodes ( Meloidogyne spp.). Endospores of P. penetrans attach to the cuticle of second-stage juveniles (J2) and subsequently sterilize infected females. When encumbered by large numbers of spores, juveniles are less mobile and their ability to infect roots is reduced. This study looked at different factors that influence spore attachment of P. penetrans to the root-knot nematode Meloidogyne arenaria . Pretreatment of J2 with root exudates of eggplant ( Solanum melongena cv. Black beauty) reduced spore attachment compared with pretreatment with phosphate-buffered saline (PBS), suggesting that the nematode surface coat was altered or the spore recognition domains on the nematode surface were blocked. Spore attachment was equally reduced following exposure to root exudates from both host and nonhost plants for M. arenaria , indicating a common signal that affects spore attachment. Although phytohormones have been shown to influence the lipophilicity of the nematode surface coat, auxins and kinetins did not affect spore attachment compared with PBS. Root exudates reduced spore attachment more in sterilized soil than in natural soil. Sterilization may have eliminated microbes that consume root exudates, or altered the chemical components of the soil solution or root exudates. Root exudates caused a greater decrease in spore attachment in loamy sand than in a sandy loam soil. The sandy loam had higher clay content than the loamy sand, which may have resulted in more adsorption of compounds in the root exudates that affect spore attachment. The components of the root exudates could have also been modified by soil type. The results of this study demonstrate that root exudates can decrease the attachment of P. penetrans endospores to root-knot nematodes, indicating that when these nematodes enter the root zone their susceptibility to spore attachment may decrease.

Evapotranspiration and remote sensing

NASA Technical Reports Server (NTRS)

Schmugge, T. J.; Gurney, R.

1982-01-01

There are three things required for evapotranspiration to occur: (1) energy (580 cal/gm) for the change of phase of the water; (2) a source of the water, i.e., adequate soil moisture in the surface layer or in the root zone of the plant; and (3) a sink for the water, i.e., a moisture deficit in the air above the ground. Remote sensing can contribute information to the first two of these conditions by providing estimates of solar insolation, surface albedo, surface temperature, vegetation cover, and soil moisture content. In addition there have been attempts to estimate precipitation and shelter air temperature from remotely sensed data. The problem remains to develop methods for effectively using these sources of information to make large area estimates of evapotranspiration.

Morphological, anatomical, and ultrastructural changes (visualized through scanning electron microscopy) induced in Triticum aestivum by Pb²⁺ treatment.

PubMed

Kaur, Gurpreet; Singh, Harminder Pal; Batish, Daizy Rani; Kohli, Ravinder Kumar

2014-11-01

Lead (Pb) causes severe damage to crops, ecosystems, and humans, and alters the physiology and biochemistry of various plant species. It is hypothesized that Pb-induced metabolic alterations could manifest as structural variations in the roots of plants. In light of this, the morphological, anatomical, and ultrastructural variations (through scanning electron microscopy, SEM) were studied in 4-day-old seedlings of Triticum aestivum grown under Pb stress (0, 8, 16, 40, and 80 mg Pb(2+) l(-1); mild to highly toxic). The toxic effect was more pronounced in radicle growth than on the plumule growth. The SEM of the root of T. aestivum depicted morphological alterations and surface ultrastructural changes. Compared to intact and uniform surface cells in the control roots, cells were irregular and desiccated in Pb(2+)-treated roots. In Pb(2+)-treated roots, the number of root hairs increased manifold, showing dense growth, and these were apparently longer. Apart from the deformity in surface morphology and anatomy of the roots in response to Pb(2+) toxicity, considerable anatomical alterations were also observed. Pb(2+)-treated root exhibited signs of injury in the form of cell distortion, particularly in the cortical cells. The endodermis and pericycle region showed loss of uniformity post Pb(2+) exposure (at 80 mg l(-1) Pb(2+)). The cells appeared to be squeezed with greater depositions observed all over the tissue. The study concludes that Pb(2+) treatment caused structural anomalies and induced anatomical and surface ultrastructural changes in T. aestivum.

Molecular genetics of root gravitropism and waving in Arabidopsis thaliana

NASA Technical Reports Server (NTRS)

Sedbrook, J.; Boonsirichai, K.; Chen, R.; Hilson, P.; Pearlman, R.; Rosen, E.; Rutherford, R.; Batiza, A.; Carroll, K.; Schulz, T.;

Environmental and physiological effects on grouping of drought-tolerant and susceptible rice varieties related to rice (Oryza sativa) root hydraulics under drought.

PubMed

Henry, Amelia; Wehler, Regina; Grondin, Alexandre; Franke, Rochus; Quintana, Marinell

2016-05-02

Root hydraulic limitations (i.e. intra-plant restrictions to water movement) may be related to crop performance under drought, and groupings in the hydraulic function of drought-tolerant and drought-susceptible rice (Oryza sativa) varieties have been previously reported. This study aimed to better understand the environmental and physiological relationships with rice root hydraulics under drought. Xylem sap bleeding rates in the field (g sap g -1 shoot ) were measured on seasonal and diurnal time frames, during which time environmental conditions were monitored and physiological measurements were conducted. Complementary experiments on the effects of vapour pressure deficit (VPD) on root hydraulic conductivity and on transpiration rates of de-rooted tillers were conducted in growth chambers. The diurnal effects on bleeding rate were more closely related to irradiance than VPD, and VPD effects on root hydraulic conductivity measured on 21-day-old plants were due to effects on plant growth including root surface area, maximum root depth and root:shoot ratio. Leaf osmotic potential was related to the grouping of drought-tolerant and drought-susceptible varieties in rice root hydraulics, and these groupings were independent of differences in phenology. Low single-tiller bleeding rates were observed under high evapo-transpirational demand, higher bleeding rates were observed at more negative leaf osmotic potentials in drought-susceptible varieties, and drought-tolerant and susceptible varieties differed in the VPD-induced increase in transpiration rates of de-rooted tillers. Low root suberin amounts in some of the drought-susceptible varieties may have resulted in higher ion transport, as evidenced by higher sap K + concentration and higher bleeding rates in those varieties. These results provide evidence of the environmental effects on shoots that can influence root hydraulics. The consistent groupings of drought-tolerant and susceptible varieties suggest that traits affecting plant osmotic status may regulate root hydraulic response to drought in rice. © The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Effect of ND:YAG laser irradiation and root planing on the root surface: structural and thermal effects.

PubMed

Wilder-Smith, P; Arrastia, A M; Schell, M J; Liaw, L H; Grill, G; Berns, M W

1995-12-01

Effects of ND:YAG laser irradiation on untreated and root planed tooth roots were investigated to determine whether a cleaning effect and/or removal of smear layer could be achieved without concomitant microstructural or thermal damage. Sixty (60) healthy extracted teeth were either untreated, irradiated only, root planed only, or irradiated and root planed. Intra-pulpal and surface temperatures were monitored during irradiation, then SEM was performed. Smear layer elimination was achieved without inducing hard tissue microstructural damage at 5W, using pulse durations and intervals of 0.1 s, a fluence of 0.77 J/cm2, and a total energy density of approximately 700 J/cm2. However, these results were not consistent in all samples. At these parameters, intra-pulpal temperature increases of 9 to 22 degrees C and surface temperature increases of 18 to 36 degrees C were recorded. Thus, despite their effectiveness for smear layer removal, these parameters may not be appropriate for clinical use as an adjunct to conventional periodontal therapy.

Effects of surface functionalization on the electronic and structural properties of carbon nanotubes: A computational approach

NASA Astrophysics Data System (ADS)

Ribeiro, M. S.; Pascoini, A. L.; Knupp, W. G.; Camps, I.

2017-12-01

Carbon nanotubes (CNTs) have important electronic, mechanical and optical properties. These features may be different when comparing a pristine nanotube with other presenting its surface functionalized. These changes can be explored in areas of research and application, such as construction of nanodevices that act as sensors and filters. Following this idea, in the current work, we present the results from a systematic study of CNT's surface functionalized with hydroxyl and carboxyl groups. Using the entropy as selection criterion, we filtered a library of 10k stochastically generated complexes for each functional concentration (5, 10, 15, 20 and 25%). The structurally related parameters (root-mean-square deviation, entropy, and volume/area) have a monotonic relationship with functionalization concentration. Differently, the electronic parameters (frontier molecular orbital energies, electronic gap, molecular hardness, and electrophilicity index) present and oscillatory behavior. For a set of concentrations, the nanotubes present spin polarized properties that can be used in spintronics.

In Vitro Morphogenesis of Arabidopsis to Search for Novel Endophytic Fungi Modulating Plant Growth.

PubMed

Dovana, Francesco; Mucciarelli, Marco; Mascarello, Maurizio; Fusconi, Anna

2015-01-01

Fungal endophytes have shown to affect plant growth and to confer stress tolerance to the host; however, effects of endophytes isolated from water plants have been poorly investigated. In this study, fungi isolated from stems (stem-E) and roots (root-E) of Mentha aquatica L. (water mint) were identified, and their morphogenetic properties analysed on in vitro cultured Arabidopsis (L.) Heynh., 14 and 21 days after inoculation (DAI). Nineteen fungi were analysed and, based on ITS analysis, 17 isolates showed to be genetically distinct. The overall effect of water mint endophytes on Arabidopsis fresh (FW) and dry weight (DW) was neutral and positive, respectively, and the increased DW, mainly occurring 14 DAI, was possibly related to plant defence mechanism. Only three fungi increased both FW and DW of Arabidopsis at 14 and 21 DAI, thus behaving as plant growth promoting (PGP) fungi. E-treatment caused a reduction of root depth and primary root length in most cases and inhibition-to-promotion of root area and lateral root length, from 14 DAI. Only Phoma macrostoma, among the water mint PGP fungi, increased both root area and depth, 21 DAI. Root depth and area 14 DAI were shown to influence DWs, indicating that the extension of the root system, and thus nutrient uptake, was an important determinant of plant dry biomass. Reduction of Arabidopsis root depth occurred to a great extent when plants where treated with stem-E while root area decreased or increased under the effects of stem-E and root-E, respectively, pointing to an influence of the endophyte origin on root extension. M. aquatica and many other perennial hydrophytes have growing worldwide application in water pollution remediation. The present study provided a model for directed screening of endophytes able to modulate plant growth in the perspective of future field applications of these fungi.

Plant root and shoot dynamics during subsurface obstacle interaction

NASA Astrophysics Data System (ADS)

Conn, Nathaniel; Aguilar, Jeffrey; Benfey, Philip; Goldman, Daniel

As roots grow, they must navigate complex underground environments to anchor and retrieve water and nutrients. From gravity sensing at the root tip to pressure sensing along the tip and elongation zone, the complex mechanosensory feedback system of the root allows it to bend towards greater depths and avoid obstacles of high impedance by asymmetrically suppressing cell elongation. Here we investigate the mechanical and physiological responses of roots to rigid obstacles. We grow Maize, Zea mays, plants in quasi-2D glass containers (22cm x 17cm x 1.4cm) filled with photoelastic gel and observe that, regardless of obstacle interaction, smaller roots branch off the primary root when the upward growing shoot (which contains the first leaf) reaches an average length of 40 mm, coinciding with when the first leaf emerges. However, prior to branching, contacts with obstacles result in reduced root growth rates. The growth rate of the root relative to the shoot is sensitive to the angle of the obstacle surface, whereby the relative root growth is greatest for horizontally oriented surfaces. We posit that root growth is prioritized when horizontal obstacles are encountered to ensure anchoring and access to nutrients during later stages of development. NSF Physics of Living Systems.

Global patterns of groundwater table depth.

PubMed

Fan, Y; Li, H; Miguez-Macho, G

2013-02-22

Shallow groundwater affects terrestrial ecosystems by sustaining river base-flow and root-zone soil water in the absence of rain, but little is known about the global patterns of water table depth and where it provides vital support for land ecosystems. We present global observations of water table depth compiled from government archives and literature, and fill in data gaps and infer patterns and processes using a groundwater model forced by modern climate, terrain, and sea level. Patterns in water table depth explain patterns in wetlands at the global scale and vegetation gradients at regional and local scales. Overall, shallow groundwater influences 22 to 32% of global land area, including ~15% as groundwater-fed surface water features and 7 to 17% with the water table or its capillary fringe within plant rooting depths.

Enhancing phosphorus and zinc acquisition efficiency in rice: a critical review of root traits and their potential utility in rice breeding

PubMed Central

Rose, T. J.; Impa, S. M.; Rose, M. T.; Pariasca-Tanaka, J.; Mori, A.; Heuer, S.; Johnson-Beebout, S. E.; Wissuwa, M.

2013-01-01

Background Rice is the world's most important cereal crop and phosphorus (P) and zinc (Zn) deficiency are major constraints to its production. Where fertilizer is applied to overcome these nutritional constraints it comes at substantial cost to farmers and the efficiency of fertilizer use is low. Breeding crops that are efficient at acquiring P and Zn from native soil reserves or fertilizer sources has been advocated as a cost-effective solution, but would benefit from knowledge of genes and mechanisms that confer enhanced uptake of these nutrients by roots. Scope This review discusses root traits that have been linked to P and Zn uptake in rice, including traits that increase mobilization of P/Zn from soils, increase the volume of soil explored by roots or root surface area to recapture solubilized nutrients, enhance the rate of P/Zn uptake across the root membrane, and whole-plant traits that affect root growth and nutrient capture. In particular, this review focuses on the potential for these traits to be exploited through breeding programmes to produce nutrient-efficient crop cultivars. Conclusions Few root traits have so far been used successfully in plant breeding for enhanced P and Zn uptake in rice or any other crop. Insufficient genotypic variation for traits or the failure to enhance nutrient uptake under realistic field conditions are likely reasons for the limited success. More emphasis is needed on field studies in mapping populations or association panels to identify those traits and underlying genes that are able to enhance nutrient acquisition beyond the level already present in most cultivars. PMID:23071218

Root Ideotype Influences Nitrogen Transport and Assimilation in Maize

PubMed Central

Dechorgnat, Julie; Francis, Karen L.; Dhugga, Kanwarpal S.; Rafalski, J. A.; Tyerman, Stephen D.; Kaiser, Brent N.

2018-01-01

Maize (Zea mays, L.) yield is strongly influenced by external nitrogen inputs and their availability in the soil solution. Overuse of nitrogen-fertilizers can have detrimental ecological consequences through increased nitrogen pollution of water and the release of the potent greenhouse gas, nitrous oxide. To improve yield and overall nitrogen use efficiency (NUE), a deeper understanding of nitrogen uptake and utilization is required. This study examines the performance of two contrasting maize inbred lines, B73 and F44. F44 was selected in Florida on predominantly sandy acidic soils subject to nitrate leaching while B73 was selected in Iowa on rich mollisol soils. Transcriptional, enzymatic and nitrogen transport analytical tools were used to identify differences in their N absorption and utilization capabilities. Our results show that B73 and F44 differ significantly in their genetic, enzymatic, and biochemical root nitrogen transport and assimilatory pathways. The phenotypes show a strong genetic relationship linked to nitrogen form, where B73 showed a greater capacity for ammonium transport and assimilation whereas F44 preferred nitrate. The contrasting phenotypes are typified by differences in root system architecture (RSA) developed in the presence of both nitrate and ammonium. F44 crown roots were longer, had a higher surface area and volume with a greater lateral root number and density than B73. In contrast, B73 roots (primary, seminal, and crown) were more abundant but lacked the defining features of the F44 crown roots. An F1 hybrid between B73 and F44 mirrored the B73 nitrogen specificity and root architecture phenotypes, indicating complete dominance of the B73 inbred. This study highlights the important link between RSA and nitrogen management and why both variables need to be tested together when defining NUE improvements in any selection program. PMID:29740466

Morphological plasticity of root growth under mild water stress increases water use efficiency without reducing yield in maize

NASA Astrophysics Data System (ADS)

Cai, Qian; Zhang, Yulong; Sun, Zhanxiang; Zheng, Jiaming; Bai, Wei; Zhang, Yue; Liu, Yang; Feng, Liangshan; Feng, Chen; Zhang, Zhe; Yang, Ning; Evers, Jochem B.; Zhang, Lizhen

2017-08-01

A large yield gap exists in rain-fed maize (Zea mays L.) production in semi-arid regions, mainly caused by frequent droughts halfway through the crop-growing period due to uneven distribution of rainfall. It is questionable whether irrigation systems are economically required in such a region since the total amount of rainfall does generally meet crop requirements. This study aimed to quantitatively determine the effects of water stress from jointing to grain filling on root and shoot growth and the consequences for maize grain yield, above- and below-ground dry matter, water uptake (WU) and water use efficiency (WUE). Pot experiments were conducted in 2014 and 2015 with a mobile rain shelter to achieve conditions of no, mild or severe water stress. Maize yield was not affected by mild water stress over 2 years, while severe stress reduced yield by 56 %. Both water stress levels decreased root biomass slightly but shoot biomass substantially. Mild water stress decreased root length but increased root diameter, resulting in no effect on root surface area. Due to the morphological plasticity in root growth and the increase in root / shoot ratio, WU under water stress was decreased, and overall WUE for both above-ground dry matter and grain yield increased. Our results demonstrate that an irrigation system might be not economically and ecologically necessary because the frequently occurring mild water stress did not reduce crop yield much. The study helps us to understand crop responses to water stress during a critical water-sensitive period (middle of the crop-growing season) and to mitigate drought risk in dry-land agriculture.

Effects of multi-directional vibrotactile feedback on vestibular-deficient postural performance during continuous multi-directional support surface perturbations.

PubMed

Sienko, K H; Balkwill, M D; Oddsson, L I E; Wall, C

2008-01-01

Single-axis vibrotactile feedback of trunk tilt provided in real-time has previously been shown to significantly reduce the root-mean-square (RMS) trunk sway in subjects with vestibular loss during single-axis perturbation. This research examines the effect of multi-directional vibrotactile feedback on postural sway during continuous multi-directional surface perturbations when the subjects' eyes are closed. Eight subjects with vestibular loss donned a multi-axis feedback device that mapped body tilt estimates onto their torsos with a 3-row by 16-column array of tactile actuators (tactors). Tactor row indicated tilt magnitude and tactor column indicated tilt direction. Root-mean-square trunk tilt, elliptical fits to trunk sway trajectory areas, percentage of time spent outside a no vibrotactile feedback zone, RMS center of pressure, and anchoring index parameters indicating intersegmental coordination were used to assess the efficacy of the multi-directional vibrotactile balance aid. Four tactor display configurations in addition to the tactors off configuration were evaluated. Subjects had significantly reduced RMS trunk sway, significantly smaller elliptical fits of the trajectory area, and spent significantly less time outside of the no feedback zone in the tactors on versus the tactors off configuration. Among the displays evaluated in this study, there was not an optimal tactor column configuration for standing tasks involving continuous surface perturbations. Furthermore, subjects performed worse when erroneous information was displayed. Therefore, a spatial resolution of 90 degrees (4 columns) seems to be as effective as a spatial resolution of 22.5 degrees (16 columns) for control of standing.

Estimation of Land Surface Fluxes and Their Uncertainty via Variational Data Assimilation Approach

NASA Astrophysics Data System (ADS)

Abdolghafoorian, A.; Farhadi, L.

2016-12-01

Accurate estimation of land surface heat and moisture fluxes as well as root zone soil moisture is crucial in various hydrological, meteorological, and agricultural applications. "In situ" measurements of these fluxes are costly and cannot be readily scaled to large areas relevant to weather and climate studies. Therefore, there is a need for techniques to make quantitative estimates of heat and moisture fluxes using land surface state variables. In this work, we applied a novel approach based on the variational data assimilation (VDA) methodology to estimate land surface fluxes and soil moisture profile from the land surface states. This study accounts for the strong linkage between terrestrial water and energy cycles by coupling the dual source energy balance equation with the water balance equation through the mass flux of evapotranspiration (ET). Heat diffusion and moisture diffusion into the column of soil are adjoined to the cost function as constraints. This coupling results in more accurate prediction of land surface heat and moisture fluxes and consequently soil moisture at multiple depths with high temporal frequency as required in many hydrological, environmental and agricultural applications. One of the key limitations of VDA technique is its tendency to be ill-posed, meaning that a continuum of possibilities exists for different parameters that produce essentially identical measurement-model misfit errors. On the other hand, the value of heat and moisture flux estimation to decision-making processes is limited if reasonable estimates of the corresponding uncertainty are not provided. In order to address these issues, in this research uncertainty analysis will be performed to estimate the uncertainty of retrieved fluxes and root zone soil moisture. The assimilation algorithm is tested with a series of experiments using a synthetic data set generated by the simultaneous heat and water (SHAW) model. We demonstrate the VDA performance by comparing the (synthetic) true measurements (including profile of soil moisture and temperature, land surface water and heat fluxes, and root water uptake) with VDA estimates. In addition, the feasibility of extending the proposed approach to use remote sensing observations is tested by limiting the number of LST observations and soil moisture observations.

In vitro propagation of fraser photinia using Azospirillum-mediated root development.

PubMed

Llorente, Berta E; Larraburu, Ezequiel E

2013-01-01

Fraser photinia (Photinia × fraseri Dress.) is a woody plant of high ornamental value. The traditional propagation system for photinia is by rooting apical cuttings using highly concentrated auxin treatments. However, photinia micropropagation is an effective alternative to traditional in vivo propagation which is affected by the seasonal supply of cuttings, the long time required to obtain new plants, and the difficulties in rooting some clones.A protocol for in vitro propagation of fraser photinia using the plant growth-promoting ability of some rhizobacteria is described here. Bacterial inoculation is a new tool in micropropagation protocols that improves plant development in in vitro culture. Shoots culture on a medium containing MS macro- and microelements, Gamborg's vitamins (BM), N (6)-benzyladenine (BA, 11.1 μM), and gibberellic acid (1.3 μM) produce well-established explants. Proliferation on BM medium supplemented with 4.4 μM BA results in four times the number of shoots per initial shoot that develops monthly. Consequently, there is a continuous supply of plant material since shoot production is independent of season. Azospirillum brasilense inoculation, after 49.2 μM indole-3-butyric acid pulse treatment, stimulates early rooting of photinia shoots and produces significant increase in root fresh and dry weights, root surface area, and shoot fresh and dry weights in comparison with controls. Furthermore, inoculated in vitro photinia plants show anatomical and morphological changes that might lead to better adaptation in ex vitro conditions after transplanting, compared with the control plants.

[Effects of different crop rotations on growth of continuous cropping sorghum and its rhizosphere soil micro-environment.

PubMed

Wang, Jin Song; Fan, Fang Fang; Guo, Jun; Wu, Ai Lian; Dong, Er Wei; Bai, Wen Bin; Jiao, Xiao Yan

2016-07-01

The effects of crop rotation on sorghum [Sorghum biocolor (L) Moench] growth, rhizosphere microbial community and the activity of soil enzymes for successive crops of sorghum were evaluated. Five years of continuous monoculture sorghum as the control (CK) was compared to alfalfa and scallion planted in the fourth year. The results showed that incorporation of alfalfa and scallion into the rotation significantly improved sorghum shoot growth. Specifically, sorghum grain yield increased by 16.5% in the alfalfa rotation plots compared to the CK. The rotations also increased sorghum root system growth, with alfalfa or scallion rotation increasing sorghum total root length by 0.3 and 0.4 times, total root surface area by 0.6 and 0.5 times, root volume by 1.2 and 0.6 times, and root biomass by 1.0 and 0.3 times, respectively. Alfalfa rotation also expanded sorghum root distribution below the 10 cm soil depth. A Biolog analysis on biome functions in the sorghum flowering period indicated significantly higher microbial activity in the rotation plots. The alfalfa and scallion rotation increased the Shannon index by 0.2 and 0.1 times compared to the CK, and improved the sucrose activity in the rhizosphere soil. It was concluded that including alfalfa in rotation with sorghum improved sorghum rhizosphere soil environment, enhanced soil microbial enzyme activity, alleviated the obstacle of continuous cropping and thus increased the sorghum yield.

Surface area and volume determination of subgingival calculus using laser fluorescence.

PubMed

Shakibaie, Fardad; Walsh, Laurence J

2014-03-01

Visible red (655 nm) laser fluorescence (LF) devices are currently used for identifying deposits of subgingival calculus on the root surfaces of teeth during dental examination and treatment; however, it is not known how the fluorescence readings produced by commercially available LF systems correlate to the nature of the deposits. This laboratory study explored the correlation between LF digital readings and the surface area and volume of subgingival calculus deposits on teeth. A collection of 30 extracted human posterior teeth with various levels of subgingival deposits of calculus across 240 sites were used in a clinical simulation, with silicone impression material used to replicate periodontal soft tissues. The teeth were scored by two examiners by using three commercial LF systems (DIAGNOdent, DIAGNOdent Pen and KEY3). The silicone was removed, and the teeth were removed for photography at × 20 magnification under white or ultraviolet light. The surface area, thickness, and volume were calculated, and both linear least squares regression and nonlinear (Spearman's rank method) correlation coefficients were determined. Visible red LF digital readings showed better correlation to calculus volume than to surface area. Overall, the best performance was found for the KEY3 system (Spearman coefficient 0.59), compared to the Classic DIAGNOdent (0.56) and the DIAGNOdent Pen (0.49). These results indicate that while visible red LF systems vary somewhat in performance, their LF readings provide a useful estimation of the volume of subgingival calculus deposits present on teeth.

Soil moisture retrieval by active/passive microwave remote sensing data

NASA Astrophysics Data System (ADS)

Wu, Shengli; Yang, Lijuan

2012-09-01

This study develops a new algorithm for estimating bare surface soil moisture using combined active / passive microwave remote sensing on the basis of TRMM (Tropical Rainfall Measuring Mission). Tropical Rainfall Measurement Mission was jointly launched by NASA and NASDA in 1997, whose main task was to observe the precipitation of the area in 40 ° N-40 ° S. It was equipped with active microwave radar sensors (PR) and passive sensor microwave imager (TMI). To accurately estimate bare surface soil moisture, precipitation radar (PR) and microwave imager (TMI) are simultaneously used for observation. According to the frequency and incident angle setting of PR and TMI, we first need to establish a database which includes a large range of surface conditions; and then we use Advanced Integral Equation Model (AIEM) to calculate the backscattering coefficient and emissivity. Meanwhile, under the accuracy of resolution, we use a simplified theoretical model (GO model) and the semi-empirical physical model (Qp Model) to redescribe the process of scattering and radiation. There are quite a lot of parameters effecting backscattering coefficient and emissivity, including soil moisture, surface root mean square height, correlation length, and the correlation function etc. Radar backscattering is strongly affected by the surface roughness, which includes the surface root mean square roughness height, surface correlation length and the correlation function we use. And emissivity is differently affected by the root mean square slope under different polarizations. In general, emissivity decreases with the root mean square slope increases in V polarization, and increases with the root mean square slope increases in H polarization. For the GO model, we found that the backscattering coefficient is only related to the root mean square slope and soil moisture when the incident angle is fixed. And for Qp Model, through the analysis, we found that there is a quite good relationship between Qpparameter and root mean square slope. So here, root mean square slope is a parameter that both models shared. Because of its big influence to backscattering and emissivity, we need to throw it out during the process of the combination of GO model and Qp model. The result we obtain from the combined model is the Fresnel reflection coefficient in the normal direction gama(0). It has a good relationship with the soil dielectric constant. In Dobson Model, there is a detailed description about Fresnel reflection coefficient and soil moisture. With the help of Dobson model and gama(0) that we have obtained, we can get the soil moisture that we want. The backscattering coefficient and emissivity data used in combined model is from TRMM/PR, TMI; with this data, we can obtain gama(0); further, we get the soil moisture by the relationship of the two parameters-- gama(0) and soil moisture. To validate the accuracy of the retrieval soil moisture, there is an experiment conducted in Tibet. The soil moisture data which is used to validate the retrieval algorithm is from GAME-Tibet IOP98 Soil Moisture and Temperature Measuring System (SMTMS). There are 9 observing sites in SMTMS to validate soil moisture. Meanwhile, we use the SMTMS soil moisture data obtained by Time Domain Reflectometer (TDR) to do the validation. And the result shows the comparison of retrieval and measured results is very good. Through the analysis, we can see that the retrieval and measured results in D66 is nearly close; and in MS3608, the measured result is a little higher than retrieval result; in MS3637, the retrieval result is a little higher than measured result. According to the analysis of the simulation results, we found that this combined active and passive approach to retrieve the soil moisture improves the retrieval accuracy.

Modelling orange tree root water uptake active area by minimally invasive ERT data and transpiration measurements

NASA Astrophysics Data System (ADS)

Vanella, Daniela; Boaga, Jacopo; Perri, Maria Teresa; Consoli, Simona; Cassiani, Giorgio

2015-04-01

The comprehension of the hydrological processes involving plant root dynamics is crucial for implementing water saving measures in agriculture. This is particular urgent in areas, like those Mediterranean, characterized by scarce water availability. The study of root water dynamics should not be separated from a more general analysis of the mass and energy fluxes transferred in the soil-plant-atmosphere continuum. In our study, in order to carry this inclusive approach, minimal invasive 3D time-lapse electrical resistivity tomography (ERT) for soil moisture estimation was combined with plant transpiration fluxes directly measured with Sap Flow (SF) techniques and Eddy Covariance methods, and volumetric soil moisture measurements by TDR probes. The main objective of this inclusive approach was to accurately define root-zone water dynamics and individuate the root-area effectively active for water and nutrient uptake process. The monitoring was carried out in Eastern Sicily (south Italy) in summers 2013 and 2014, within an experimental orange orchard farm. During the first year of experiment (October 2013), ERT measurements were carried out around the pertinent volume of one fully irrigated tree, characterized by a vegetation ground cover of 70%; in the second year (June 2014), ERT monitoring was conducted considering a cutting plant, thus to evaluate soil water dynamics without the significant plant transpiration contribution. In order to explore the hydrological dynamics of the root zone volume surrounded by the monitored tree, the resistivity data acquired during the ERT monitoring were converted into soil moisture content distribution by a laboratory calibration based on the soil electrical properties as a function of moisture content and pore water electrical conductivity. By using ERT data in conjunction with the agro-meteorological information (i.e. irrigation rates, rainfall, evapotranspiration by Eddy Covariance, transpiration by Sap Flow and soil moisture content by TRD) of the test area, a spatially distributed one-dimensional (1D) model that solves the Richards' equation was applied; in the model the van Genuchten parameters were obtained by laboratory analysis of soil water retention and soil permeability at saturation. Results of the 1D model were successfully compared with both ERT-based soil moisture dynamics and TDR measurements of soil moisture. The modelling allows to defining the soil volume interested by root water uptake process and its extent. In particular, this volume results significantly smaller (i.e. surface area of 1.75 m2, with 0.4 m cm thickness) than expected, considering the design of the drip irrigation scheme adopted in the farm. The obtained results confirm that ERT is a technique that (i) can provide a lot of information on small scale and vegetation related processes; (ii) the integration with physical modelling is essential to capture the meaning of space-time signal changes; (iii) in the case of the orange orchard, this approach shows that about half of the irrigated water is wasted.

Coastal retracking using along-track echograms and its dependency on coastal topography

NASA Astrophysics Data System (ADS)

Ichikawa, K.; Wang, X.

2017-12-01

Although the Brown mathematical model is the standard model for waveform retracking over open oceans, coastal waveforms usually deviate from open ocean waveform shapes due to inhomogeneous surface reflections within altimeter footprints, and thus cannot be directly interpreted by the Brown model. Generally, the two primary sources of heterogeneous surface reflections are land surfaces and bright targets such as calm surface water. The former reduces echo power, while the latter often produces particularly strong echoes. In previous studies, sub-waveform retrackers, which use waveform samples collected from around leading edges in order to avoid trailing edge noise, have been recommended for coastal waveform retracking. In the present study, the peaky-type noise caused by fixed-point bright targets is explicitly detected and masked using the parabolic signature in the sequential along-track waveforms (or, azimuth-range echograms). Moreover, the power deficit of waveform trailing edges caused by weak land reflections is compensated for by estimating the ratio of sea surface area within each annular footprint in order to produce pseudo-homogeneous reflected waveforms suitable for the Brown model. Using this method, Jason-2 altimeter waveforms are retracked in several coastal areas. Our results show that both the correlation coefficient and root mean square difference between the derived sea surface height anomalies and tide gauge records retain similar values at the open ocean (0.9 and 20 cm) level, even in areas approaching 3 km from coastlines, which is considerably improved from the 10 km correlation coefficient limit of the conventional MLE4 retracker and the 7 km sub-waveform ALES retracker limit. These values, however, depend on the coastal topography of the study areas because the approach distance limit increases (decreases) in areas with complicated (straight) coastlines

The application of soil amendments benefits to the reduction of phosphorus depletion and the growth of cabbage and corn.

PubMed

Liu, Wei; Ji, Hongli; Kerr, Philip; Wu, Yonghong; Fang, Yanming

2015-11-01

The loss of phosphorus from agricultural intensive areas can cause ecological problems such as eutrophication in downstream surface waters. Therefore, the purpose of this study is to control the phosphorus loss using environmentally benign soil amendments, viz, ferrous sulfate (FES), aluminum sulfate (ALS), and polyacrylamide (PAM). The phosphorus concentration changes in soil and leaching solution, the morphological index of plant (including stem and root), and root activity and quality (represented by chlorophyll and soluble sugar) at different growth stages of cabbage (Brassica oleracea L. var. capitata L.) were monitored in a pilot experiment. Phosphorus contents in soil and runoff were also investigated in field experiments cultivated with corn (Zea mays L.). The results show that the application of these amendments improved the phosphorus uptake by cabbage and corn, resulting in the enhanced morphologies of root and stem as well as the root activity at the early and middle stages of cabbage growth. The soil total phosphorus and available phosphorus in soils treated with FES, ALS, and PAM declined, resulting in lower concentrations of phosphorus in the leachate and the soil runoff. During the use of the soil amendments, the cabbage quality measures, determined as chlorophyll and soluble sugar in leaves, were not significantly different from those in the control. It is suggested that the application of these soil amendments is safe for cabbage production under single season cropping conditions, and the use of these three amendments is a promising measure to reduce phosphorus loss in intensive agricultural areas.

Effects of adhesions of amorphous Fe and Al hydroxides on surface charge and adsorption of K+ and Cd2+ on rice roots.

PubMed

Liu, Zhao-Dong; Wang, Hai-Cui; Zhou, Qin; Xu, Ren-Kou

2017-11-01

Iron (Fe) and aluminum (Al) hydroxides in variable charge soils attached to rice roots may affect surface-charge properties and subsequently the adsorption and uptake of nutrients and toxic metals by the roots. Adhesion of amorphous Fe and Al hydroxides onto rice roots and their effects on zeta potential of roots and adsorption of potassium (K + ) and cadmium (Cd 2+ ) by roots were investigated. Rice roots adsorbed more Al hydroxide than Fe hydroxide because of the greater positive charge on Al hydroxide. Adhesion of Fe and Al hydroxides decreased the negative charge on rice roots, and a greater effect of the Al hydroxide. Consequently, adhesion of Fe and Al hydroxides reduced the K + and Cd 2+ adsorption by rice roots. The results of attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) and desorption of K + and Cd 2+ from rice roots indicated that physical masking by Fe and Al hydroxides and diffuse-layer overlapping between the positively-charged hydroxides and negatively-charged roots were responsible for the reduction of negative charge on roots induced by adhesion of the hydroxides. Therefore, the interaction between Fe and Al hydroxides and rice roots reduced negative charge on roots and thus inhibited their adsorption of nutrient and toxic cations. Copyright © 2017 Elsevier Inc. All rights reserved.

Viewing forests from below: fine root mass declines relative to leaf area in aging lodgepole pine stands.

PubMed

Schoonmaker, A S; Lieffers, V J; Landhäusser, S M

2016-07-01

In the continued quest to explain the decline in productivity and vigor with aging forest stands, the most poorly studied area relates to root system change in time. This paper measures the wood production, root and leaf area (and mass) in a chronosequence of fire-origin lodgepole pine (Pinus contorta Loudon) stands consisting of four age classes (12, 21, 53, and ≥100 years), each replicated ~ five times. Wood productivity was greatest in the 53-year-old stands and then declined in the ≥100-year-old stands. Growth efficiency, the quantity of wood produced per unit leaf mass, steadily declined with age. Leaf mass and fine root mass plateaued between the 53- and ≥100-year-old stands, but leaf area index actually increased in the older stands. An increase in the leaf area index:fine root area ratio supports the idea that older stand are potentially limited by soil resources. Other factors contributing to slower growth in older stands might be lower soil temperatures and increased self-shading due to the clumped nature of crowns. Collectively, the proportionally greater reduction in fine roots in older stands might be the variable that predisposes these forests to be at a potentially greater risk of stress-induced mortality.

Molecular dynamics study of biodegradation of azo dyes via their interactions with AzrC azoreductase.

PubMed

Haghshenas, Hamed; Kay, Maryam; Dehghanian, Fariba; Tavakol, Hossein

2016-01-01

Azo dyes are one of the most important class of dyes, which have been widely used in industries. Because of the environmental pollution of azo dyes, many studies have been performed to study their biodegradation using bacterial systems. In present work, the AzrC of mesophilic gram-positive Bacillus sp. B29 has been considered to study its interaction with five common azo dyes (orange G, acid red 88, Sudan I, orange I, and methyl red). The molecular dynamics simulations have been employed to study the interaction between AzrC and azo dyes. The trajectory was confirmed using root mean square deviation and the root mean square fluctuation analyses. Then, the hydrogen bond and alanine scanning analyses were performed to reveal active site residues. Phe105 (A), Phe125 (B), Phe172 (B), and Pro132 (B) have been found as the most important hydrophobic residues whereas Asn104 (A), Tyr127 (B), and Asn187 (A) have key role in making hydrogen bond. The results of molecular mechanics Poisson-Boltzmann surface area and molecular mechanics generalized Born surface area calculations proved that the hydrophobic azo dyes like Acid red 88 binds more tightly to the AzrC protein. The calculated data suggested MR A 121 (B) I as a potential candidate for improving the AzrC-MR interactions.

Body surface area as a key determinant of aortic root and arch dimensions in a population-based study.

PubMed

Wang, Yan-Li; Wang, Qing-Ling; Wang, Liang; Wu, Ying-Biao; Wang, Zhi-Bin; Cameron, James; Liang, Yu-Lu

2013-02-01

The associations between the aortic dimensions (of the aortic sinus, aortic annulus and aortic arch) and physiological variables have not been established in the Chinese population. The present study examined the associations among physiological variables to determine the aortic root and arch dimensions echocardiographically. The diameters of the aortic sinus, annulus and arch were measured in 1,010 subjects via 2-D echocardiography with a 3.5-MHz transducer in a trans-thoracic position. The images of the aortic sinus and aortic annulus were obtained from a standard parasternal long-axis view. The maximum diameter of the valve orifice was measured at the end of systole. The aortic arch dimension was visualized in the long-axis using a suprasternal notch window and the maximum transverse diameter was measured. Epidata 3.0, Excel 2007 and SPSS version 17.0 were used to collect and analyze the data. A total of 1,010 subjects were enrolled. The mean age was 55.0±17.0 years (range of 18 to 90 years). The body surface area (BSA) was the best predictor of all the studied physiological variables and may be used to predict aortic sinus, annulus and arch dimensions independently (r=0.54, 0.37 and 0.39, respectively). Gender, blood pressure, age and BSA are significant predictors of the aortic dimensions. Of these, BSA was the best predictor.

Fungi in neotropical epiphyte roots.

PubMed

Bermudes, D; Benzing, D H

1989-01-01

Roots of thirty-eight Ecuadoran vascular epiphytes, representing eleven angiosperm families, were examined for the presence of symbiotic microorganisms. Most orchid roots contained fungal endophytes like those that regularly infect terrestrial counterparts. Hyphae were also common in and on nonorchid roots, but assignments of these relationships to known mycorrhizal morphologies was not possible in all cases. Evidence of vesicular-arbuscular mycorrhizae (VAM) existed in a number of subjects while in Ericaceae and Campanulaceae a fungal association similar to the demateaceous surface fungi (DSF) described for alpine and prarie plants was usually present. Some associations were characterized by multicellular propagules on root surfaces. The significance of these findings and the factors likely to influence occurrence and consequences of root-fungus mutualisms in tropical forest canopies are discussed. Facts and considerations that could aid future inquiry on these systems are provided.

Health of native riparian vegetation and its relation to hydrologic conditions along the Mojave River, southern California

USGS Publications Warehouse

Lines, Gregory C.

1999-01-01

The health of native riparian vegetation and its relation to hydrologic conditions were studied along the Mojave River mainly during the growing seasons of 1997 and 1998. The study concentrated on cottonwood?willow woodlands (predominantly Populus fremontii and Salix gooddingii) and mesquite bosques (predominantly Prosopis glandulosa). Tree-growth characteristics were measured at 16 cottonwood?willow woodland sites and at 3 mesquite bosque sites. Density of live and dead trees, tree diameter and height, canopy density, live-crown volume, leaf-water potential, leaf-area index, mortality, and reproduction were measured or noted at each site. The sites included healthy and reproducing woodlands and bosques, stressed woodlands and bosques with no reproduction, and woodlands and bosques with high mortality. Tree roots were studied at seven sites to determine the vertical distribution of the root system and their relation to the water table at healthy, stressed, and high-mortality cottonwood?willow woodlands. In the six trenches that were dug for this study in May 1997, no cottonwood roots were observed that reached the water table. The root systems of healthy trees typically ended 1 to 2 feet above the water table. At sites with high mortality, the main root mass was commonly 7 to 8 feet above the water table. Water-table depth was monitored at each of the study sites. In addition, volumetric soil moisture and soil-water potential were monitored at varying depths at three cottonwood?willow woodland study sites and at two mesquite bosque sites. Ground, soil, river, lake, and plant (xylem sap) water were analyzed for concentrations of stable hydrogen and oxygen isotopes to determine the source of water used by the trees. On the basis of the root-distribution, soil- and leaf-water potential, and isotope data, it was concluded that cottonwood, willow, and mesquite trees mainly rely on ground water for their perennial sustained supply of water. The trees mainly utilize ground water that has moved upward from the water table into the capillary fringe and into unsaturated soil nearer to land surface. Most precipitation (average is 4 to 6 inches per year) is lost by evaporation and by transpiration of shallow-rooted xeric plants, and very little reaches the root zone of trees along the Mojave River. Water-table depth had no strong correlation to many individual tree-growth characteristics, such as density, diameter, height, and live-crown volume. However, leaf-area index (corrected for stem area) of both healthy and stressed cottonwood?willow woodlands had a highly significant statistical relation to water-table depth, and a curvilinear regression model was defined. As in cottonwood?willow woodlands, leaf-area index of mesquite bosques also decreased with increased water-table depth. However, because of the small number of sites, no significant statistical relation could be defined for mesquite bosques. Because it can be accurately measured repeatedly at the same locations, leaf-area index (corrected for stem area) is recommended as the primary growth characteristic that should be monitored. Future vegetation changes along the Mojave River can be quantified using the sites established for this study. Mortality was as high as 39 percent in healthy cottonwood?willow woodlands, but mortality of 50 to 100 percent was common where water-table depth was greater than about 7 feet or in areas where permanent water-table declines greater than about 5 feet had occurred. At a healthy mesquite bosque where the water-table depth ranged from about 8 to 11 feet, mortality was about 20 percent. Where the water table had been lowered an additional 10 to 25 feet by pumping, mortality of the mesquite was extremely high (80 to 99 percent). On the basis of observations of plant reproduction, it was concluded that established cottonwood?willow woodlands probably will reproduce, mainly by root sprouting of mature trees, if the water-t

Assessment of lead, cadmium, and zinc contamination of roadside soils, surface films, and vegetables in Kampala City, Uganda.

PubMed

Nabulo, Grace; Oryem-Origa, Hannington; Diamond, Miriam

2006-05-01

The relationship between traffic density and trace metal concentrations in roadside soils, surface films, and a selected vegetable weed, Amaranthus dubius Mart. Ex Thell., was determined in 11 farming sites along major highways around Kampala City in Uganda. Surface soil, atmospherically deposited surface films on windows, and leaves of Amaranthus dubius were sampled at known distances from the roads and analyzed for lead (Pb), zinc (Zn), and cadmium (Cd) using flame atomic absorption spectrophotometry. Atmospherically deposited trace metal particulates were sampled using window glass as an inert, passive collector. Total trace metal concentrations in soils ranged from 30.0+/-2.3 to 64.6+/-11.7 mg/kg Pb, 78.4+/-18.4 to 265.6+/-63.2 mg/kg Zn, and 0.8+/-0.13 to 1.40+/-0.16 mg/kg Cd. Total trace metal levels in soil decreased rapidly with distance from the road. Total Pb decreased with distance up to 30 m from the road, where it reached a background soil concentration of 28 mg/kg dry weight. The study found background values of 50 and 1.4 mg/kg for Zn and Cd in roadside soils, respectively. Similarly, Pb concentration in Amaranthus dubius leaves decreased with increasing distance from the road edge. The dominant pathway for Pb contamination was from atmospheric deposition, which was consistent with Pb concentrations in surface films. The mean Pb concentrations in leaves of roadside crops were higher than those in their respective roots, with the highest leaf-to-root ratio observed in the Brassica oleraceae acephala group. The lowest Pb and Zn concentrations were found in the fruit compared to the leaves of the same crops. Leaves of roadside vegetables were therefore considered a potential source of heavy metal contamination to farmers and consumers in urban areas. It is recommended that leafy vegetables should be grown 30 m from roads in high-traffic, urban areas.

Assessment of lead, cadmium, and zinc contamination of roadside soils, surface films, and vegetables in Kampala City, Uganda

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nabulo, Grace; Oryem-Origa, Hannington; Diamond, Miriam

2006-05-15

The relationship between traffic density and trace metal concentrations in roadside soils, surface films, and a selected vegetable weed, Amaranthus dubius Mart. Ex Thell., was determined in 11 farming sites along major highways around Kampala City in Uganda. Surface soil, atmospherically deposited surface films on windows, and leaves of Amaranthus dubius were sampled at known distances from the roads and analyzed for lead (Pb), zinc (Zn), and cadmium (Cd) using flame atomic absorption spectrophotometry. Atmospherically deposited trace metal particulates were sampled using window glass as an inert, passive collector. Total trace metal concentrations in soils ranged from 30.0{+-}2.3 to 64.6{+-}11.7more » mg/kg Pb, 78.4{+-}18.4 to 265.6{+-}63.2 mg/kg Zn, and 0.8{+-}0.13 to 1.40{+-}0.16 mg/kg Cd. Total trace metal levels in soil decreased rapidly with distance from the road. Total Pb decreased with distance up to 30 m from the road, where it reached a background soil concentration of 28 mg/kg dry weight. The study found background values of 50 and 1.4 mg/kg for Zn and Cd in roadside soils, respectively. Similarly, Pb concentration in Amaranthus dubius leaves decreased with increasing distance from the road edge. The dominant pathway for Pb contamination was from atmospheric deposition, which was consistent with Pb concentrations in surface films. The mean Pb concentrations in leaves of roadside crops were higher than those in their respective roots, with the highest leaf-to-root ratio observed in the Brassica oleraceae acephala group. The lowest Pb and Zn concentrations were found in the fruit compared to the leaves of the same crops. Leaves of roadside vegetables were therefore considered a potential source of heavy metal contamination to farmers and consumers in urban areas. It is recommended that leafy vegetables should be grown 30 m from roads in high-traffic, urban areas.« less

Changes in hydraulic conductivity, mechanical properties, and density reflecting the fall in strain along the lateral roots of two species of tropical trees.

PubMed

Christensen-Dalsgaard, Karen K; Ennos, Anthony R; Fournier, Meriem

2007-01-01

Roots have been described as having larger vessels and so greater hydraulic efficiency than the stem. Differences in the strength and stiffness of the tissue within the root system itself are thought to be an adaptation to the loading conditions experienced by the roots and to be related to differences in density. It is not known how potential mechanical adaptations may affect the hydraulic properties of the roots. The change in strength, stiffness, conductivity, density, sapwood area, and second moment of area distally along the lateral roots of two tropical tree species in which the strain is known to decrease rapidly was studied and the values were compared with those of the trunk. It was found that as the strain fell distally along the roots, so did the strength and stiffness of the tissue, whereas the conductivity increased exponentially. These changes appeared to be related to differences in density. In contrast to the distal-most roots, the tissue of the proximal roots had a lower conductivity and higher strength than that of the trunk. This suggests that mechanical requirements on the structure rather than the water potential gradient from roots to branches are responsible for the general pattern that roots have larger vessels than the stem. In spite of their increased transectional area, the buttressed proximal roots were subjected to higher levels of stress and had a lower total conductivity than the rest of the root system.

Assessment of the SMAP Level-4 Surface and Root-Zone Soil Moisture Product Using In Situ Measurements

USDA-ARS?s Scientific Manuscript database

The Soil Moisture Active Passive (SMAP) mission Level-4 Surface and Root-Zone Soil Moisture (L4_SM) data product is generated by assimilating SMAP L-band brightness temperature observations into the NASA Catchment land surface model. The L4_SM product is available from 31 March 2015 to present (with...

Determination of surface layer parameters at the edge of a suburban area

NASA Astrophysics Data System (ADS)

Likso, T.; Pandžić, K.

2012-05-01

Vertical wind and air temperature profile related parameters in the surface layer at the edge of suburban area of Zagreb (Croatia) have been considered. For that purpose, adopted Monin-Obukhov similarity theory and a set of observations of wind and air temperature at 2 and 10 m above ground, recorded in 2005, have been used. The root mean square differences (errors) principle has been used as a tool to estimate the effective roughness length as well as standard deviations of wind speed and wind gusts. The results of estimation are effective roughness lengths dependent on eight wind direction sectors unknown before. Gratefully to that achievement, representativeness of wind data at standard 10-m height can be clarified more deeply for an area of at least about 1 km in upwind direction from the observation site. Extrapolation of wind data for lower or higher levels from standard 10-m height are thus properly representative for a wider inhomogeneous suburban area and can be used as such in numerical models, flux and wind energy estimation, civil engineering, air pollution and climatological applications.

HYDRAULIC REDISTRIBUTION OF SOIL WATER BY ROOTS IN FORESTS OF THE PACIFIC NORTHWEST

EPA Science Inventory

One aspect of structural complexity of forest canopies is the root system structure belowground, which influences patterns of soil water utilization by trees. Deeply rooted trees and other plants can hydraulically lift water via their roots from several m below the soil surface ...

UV-B Induced Generation of Reactive Oxygen Species Promotes Formation of BFA-Induced Compartments in Cells of Arabidopsis Root Apices

PubMed Central

Yokawa, Ken; Kagenishi, Tomoko; Baluška, František

2016-01-01

UV-B radiation is an important part of the electromagnetic spectrum emitted by the sun. For much of the period of biological evolution organisms have been exposed to UV radiation, and have developed diverse mechanisms to cope with this potential stress factor. Roots are usually shielded from exposure to UV by the surrounding soil, but may nevertheless be exposed to high energy radiation on the soil surface. Due to their high sensitivity to UV-B radiation, plant roots need to respond rapidly in order to minimize exposure on the surface. In addition to root gravitropism, effective light perception by roots has recently been discovered to be essential for triggering negative root phototropism in Arabidopsis. However, it is not fully understood how UV-B affects root growth and phototropism. Here, we report that UV-B induces rapid generation of reactive oxygen species which in turn promotes the formation of BFA-induced compartments in the Arabidopsis root apex. During unilateral UV-B irradiation of roots changes in auxin concentration on the illuminated side have been recorded. In conclusion, UV-B-induced and ROS-mediated stimulation of vesicle recycling promotes root growth and induces negative phototropism. PMID:26793199

Minimal surfaces in AdS space and integrable systems

NASA Astrophysics Data System (ADS)

Burrington, Benjamin A.; Gao, Peng

2010-04-01

We consider the Pohlmeyer reduction for spacelike minimal area worldsheets in AdS5. The Lax pair for the reduced theory is found, and written entirely in terms of the A3 = D3 root system, generalizing the B2 affine Toda system which appears for the AdS4 string. For the B2 affine Toda system, we show that the area of the worlsheet is obtainable from the moduli space Kähler potential of a related Hitchin system. We also explore the Saveliev-Leznov construction for solutions of the B2 affine Toda system, and recover the rotationally symmetric solution associated to Painleve transcendent.

Effect of two storage solutions on surface topography of two root-end fillings.

PubMed

Asgary, Saeed; Eghbal, Mohammad Jafar; Parirokh, Masoud; Ghoddusi, Jamileh

2009-12-01

The effect of different storage solutions on surface topography of mineral trioxide aggregate (MTA) and new experimental cement (NEC) as root-end fillings was investigated. Twenty-four single-rooted teeth were cleaned, shaped and obturated in a same manner. After root-end resection, 3-mm deep root-end cavities were ultrasonically prepared. Samples were randomly divided into four test groups (A1-A2-B1-B2, n = 6). Root-end cavities in groups A and B were filled with MTA and NEC, respectively, and were then stored in 100% humidity for 24 h. The samples of groups 1 and 2 were, respectively, immersed in normal saline (NS) and phosphate buffer saline solutions for 1 week. The samples were imaged under stereomicroscope before and after immersion and were then investigated and analysed by scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDXA). Results showed significant difference among studied groups. Surface topography of all samples was altered by crystal formation and precipitation on root-end fillings except for group A1 (MTA-NS). SEM and EDXA results showed that the composition and structure of precipitated crystals were comparable with that of standard hydroxyapatite. It was concluded that biocompatibility, sealing ability, and cementogenic activity of MTA and probably NEC may be attributed to this fundamental bioactive reaction.

The effect of cellulose overproduction on binding and biofilm formation on roots by Agrobacterium tumefaciens.

PubMed

Matthysse, Ann G; Marry, Mazz; Krall, Leonard; Kaye, Mitchell; Ramey, Bronwyn E; Fuqua, Clay; White, Alan R

2005-09-01

Agrobacterium tumefaciens growing in liquid attaches to the surface of tomato and Arabidopsis thaliana roots, forming a biofilm. The bacteria also colonize roots grown in sterile quartz sand. Attachment, root colonization, and biofilm formation all were markedly reduced in celA and chvB mutants, deficient in production of cellulose and cyclic beta-(1,2)-D-glucans, respectively. We have identified two genes (celG and cell) in which mutations result in the overproduction of cellulose as judged by chemical fractionation and methylation analysis. Wild-type and chvB mutant strains carrying a cDNA clone of a cellulose synthase gene from the marine urochordate Ciona savignyi also overproduced cellulose. The overproduction in a wild-type strain resulted in increased biofilm formation on roots, as evaluated by light microscopy, and levels of root colonization intermediate between those of cellulose-minus mutants and the wild type. Overproduction of cellulose by a nonattaching chvB mutant restored biofilm formation and bacterial attachment in microscopic and viable cell count assays and partially restored root colonization. Although attachment to plant surfaces was restored, overproduction of cellulose did not restore virulence in the chvB mutant strain, suggesting that simple bacterial binding to plant surfaces is not sufficient for pathogenesis.

Effect of bisphosphonates on root resorption after tooth replantation - a systematic review.

PubMed

Najeeb, Shariq; Siddiqui, Fahad; Khurshid, Zohaib; Zohaib, Sana; Zafar, Muhammad Sohail; Ansari, Shazia Akbar

2017-04-01

Replantation of avulsed teeth may lead to root resorption. Bisphosphonates (BPs), a class of drugs of used to treat resorptive diseases of the bone such as osteoporosis and Paget's disease, have been observed to exert an antiresorptive effect on periodontal bone as well. The antiresorptive properties of BPs could prove them useful in preventing root resorption of replanted avulsed teeth. The aim of this systematic review was to analyze and summarize the currently available literature concerning the use of BPs in preventing root resorption of avulsed teeth. PubMed/MEDLINE, Google Scholar, ISI Web of Knowledge, and Embase databases were searched using keywords 'bisphosphonate', 'replantation', and 'tooth'. Quality assessment of each study was carried out. In addition, general characteristics and outcomes of each study were summarized. After exclusion of 116 irrelevant articles, 10 animal studies were included in this review. The majority of the studies suggest that surface application of zoledronate or alendronate reduces root resorption of replanted teeth in animal models. Surface treatment with etidronate had no significant effect on root resorption, and intracanal etidronate accelerated resorption. Surface application of zoledronate and alendronate reduces root resorption of replanted teeth in animal models. However, the efficacy of intracanal usage of BPs is still debatable. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chlorhexidine controlled-release profile after EDTA root surface etching: an in vivo study.

PubMed

Gamal, Ahmed Y; Kumper, Radi M; Sadek, Hesham S; El Destawy, Mahmoud T

2011-05-01

The main objective of the present study was to quantify chlorhexidine (CHX) release after the use of CHX-EDTA root surface treatment as a local-delivery antimicrobial vehicle. Twenty non-smoking patients clinically diagnosed as having moderate-to-severe chronic periodontitis were selected to participate in this study. After cause-related therapy, one site in every patient received defect overfill with CHX gel 2% (20 sites). In addition, twenty contralateral sites received defect fill of CHX gel after 3 minutes of 24% EDTA gel root surface etching (20 sites). Gingival crevicular fluid samples were collected at 1, 3, 7, and 14 days post-therapy. The CHX-EDTA group showed statistically significantly higher levels of CHX than those of the control group at 1, 3, and 7 days. At 14 days, the CHX-EDTA group showed 0.8 mg/mL values. The use of CHX-EDTA root surface treatment as a local-delivery antimicrobial improves CHX substantivity.

Touch and gravitropic set-point angle interact to modulate gravitropic growth in roots

NASA Technical Reports Server (NTRS)

Massa, G. D.; Gilroy, S.

2003-01-01

Plant roots must sense and respond to a variety of environmental stimuli as they grow through the soil. Touch and gravity represent two of the mechanical signals that roots must integrate to elicit the appropriate root growth patterns and root system architecture. Obstacles such as rocks will impede the general downwardly directed gravitropic growth of the root system and so these soil features must be sensed and this information processed for an appropriate alteration in gravitropic growth to allow the root to avoid the obstruction. We show that primary and lateral roots of Arabidopsis do appear to sense and respond to mechanical barriers placed in their path of growth in a qualitatively similar fashion. Both types of roots exhibited a differential growth response upon contacting the obstacle that directed the main axis of elongation parallel to the barrier. This growth habit was maintained until the obstacle was circumvented, at which point normal gravitropic growth was resumed. Thus, the gravitational set-point angle of the primary and lateral roots prior to encountering the barrier were 95 degrees and 136 degrees respectively and after growing off the end of the obstacle identical set-point angles were reinstated. However, whilst tracking across the barrier, quantitative differences in response were observed between these two classes of roots. The root tip of the primary root maintained an angle of 136 degrees to the horizontal as it traversed the barrier whereas the lateral roots adopted an angle of 154 degrees. Thus, this root tip angle appeared dependent on the gravitropic set-point angle of the root type with the difference in tracking angle quantitatively reflecting differences in initial set-point angle. Concave and convex barriers were also used to analyze the response of the root to tracking along a continuously varying surface. The roots maintained the a fairly fixed angle to gravity on the curved surface implying a constant resetting of this tip angle/tracking response as the curve of the surface changed. We propose that the interaction of touch and gravity sensing/response systems combine to strictly control the tropic growth of the root. Such signal integration is likely a critical part of growth control in the stimulus-rich environment of the soil. c2003 COSPAR. Published by Elsevier Ltd. All rights reserved.

A tillering inhibition gene influences root–shoot carbon partitioning and pattern of water use to improve wheat productivity in rainfed environments

PubMed Central

Hendriks, P.W.; Kirkegaard, J.A.; Lilley, J.M.; Gregory, P.J.; Rebetzke, G.J.

2016-01-01

Genetic modification of shoot and root morphology has potential to improve water and nutrient uptake of wheat crops in rainfed environments. Near-isogenic lines (NILs) varying for a tillering inhibition (tin) gene and representing multiple genetic backgrounds were phenotyped in contrasting, controlled environments for shoot and root growth. Leaf area, shoot and root biomass were similar until tillering, whereupon reduced tillering in tin-containing NILs produced reductions of up to 60% in total leaf area and biomass, and increases in total root length of up to 120% and root biomass to 145%. Together, the root-to-shoot ratio increased two-fold with the tin gene. The influence of tin on shoot and root growth was greatest in the cv. Banks genetic background, particularly in the biculm-selected NIL, and was typically strongest in cooler environments. A separate de-tillering study confirmed greater root-to-shoot ratios with regular tiller removal in non-tin-containing genotypes. In validating these observations in a rainfed field study, the tin allele had a negligible effect on seedling growth but was associated with significantly (P<0.05) reduced tiller number (–37%), leaf area index (–26%), and spike number (–35%) to reduce plant biomass (–19%) at anthesis. Root biomass, root-to-shoot ratio at early stem elongation, and root depth at maturity were all increased in tin-containing NILs. Soil water use was slowed in tin-containing NILs, resulting in greater water availability, greater stomatal conductance, cooler canopy temperatures, and maintenance of green leaf area during grain-filling. Together these effects contributed to increases in harvest index and grain yield. In both the controlled and field environments, the tin gene was commonly associated with increased root length and biomass, but the significant influence of genetic background and environment suggests careful assessment of tin-containing progeny in selection for genotypic increases in root growth. PMID:26494729

Effects of NaOCl, EDTA and MTAD when applied to dentine on stress distribution in post-restored roots with flared canals.

PubMed

Belli, S; Eraslan, O; Eraslan, O; Eskitascioglu, M; Eskitascioglu, G

2014-12-01

To evaluate the effect of NaOCl, EDTA and MTAD on the stress distribution and levels in roots with flared canals and three different aesthetic post systems using finite element stress analysis (FEA). Three-dimensional (3D) FEA models simulating a maxillary incisor with excessive structural loss and flared root canals were created. The dentine of the first models of each post group was assumed as homogenous, whereas the others were deemed as having their elastic modulus affected up to 100 μm deep as a result of irrigation protocol (5.25 NaOCl, 17% EDTA and MTAD for 2 h). A sound incisor tooth model was used as the control. Restorations were created according to the post system used (pre-fabricated fibre post (PFP)), polyethylene fibre (Ribbond) post and core build-up (RBP), and one-piece milled zirconia post and core (ZP). Ceramic crowns were added to the models. A 300-N static load was applied at the centre of the palatal surface of the models to calculate the stress distributions. The SolidWorks/Cosmosworks structural analysis programmes were used for FEA analysis. Results were presented by considering von Mises criteria. The analysis of the von Mises stresses revealed that RBP created less stress in the remaining root dentine when compared to PFP and ZP. ZP maintained the stresses inside its body and reduced stress on the palatal surface of the root; however, it forwarded more stress towards the apical area. NaOCl-, EDTA- and MTAD-treated dentine increased the stresses within the root structure regardless of the effect of the post system used (11-15.4 MPa for PFP, 9.5-13.02 MPa for RBP and 14.2 MPa for ZP). Amongst the irrigation solutions used, EDTA and MTAD increased the stresses more than NaOCl in all models. All the irrigation solutions showed the same stress levels and distributions in the ZP model. NaOCl-, EDTA- and MTAD- treated dentine and a rigid post with high elastic modulus may increase fracture risk in roots with flared canals by increasing the stresses within root dentine. Therefore, solutions that alter the elastic modulus of dentine less (such as NaOCl) or an individually shaped post-core system constructed with a material that has an elastic modulus close to dentine (polyethylene fibre) should be used in weak roots. © 2014 International Endodontic Journal. Published by John Wiley & Sons Ltd.

Evaluation of Blood Cell Attachment on Er:Yag Laser Applied Root Surface Using Scanning Electron Microscopy

PubMed Central

CEKICI, Ali; MADEN, Ilay; YILDIZ, Sercan; SAN, Tangul; ISIK, Gulden

2013-01-01

Background: Periodontal regeneration is dependent on the uninterrupted adhesion, maturation and absorption of fibrin clots to a periodontally compromised root surface. The modification of the root surface with different agents has been used for better fibrin clot formation and blood cell attachment. It is known that Er:YAG laser application on dentin removes the smear layer succesfully. Aim: The aim of this study is to observe blood cell attachment and fibrin network formation following ER:YAG laser irradiation on periodontally compromised root surfaces in comparison to chemical root conditioning techniques in vitro. Materials and methods: 40 dentin blocks prepared from freshly extracted periodontally compromised hopeless teeth. Specimens were divided in 5 groups; those applied with PBS, EDTA, Citric acid and Er:YAG. They were further divided into two groups: those which had received these applications, and the control group. The specimens were evaluated with scanning electron microscope and micrographs were taken. Smear layer and blood cell attachment scoring was performed. Results: In the Er:YAG laser applied group, smear layer were totally removed. In the blood applied specimens, better fibrin clot formation and blood cell attachment were observed in the Er:YAG group. In the group that had been applied with citric acid, the smear layer was also removed. The smear layer could not be fully removed in the EDTA group. Conclusion: Er:YAG laser application on the root dentin seems to form a suitable surface for fibrin clot formation and blood cell attachment. Further clinical studies to support these results are necessitated. PMID:23533017

The mechanics of delamination in fiber-reinforced composite materials. I - Stress singularities and solution structure

NASA Technical Reports Server (NTRS)

Wang, S. S.; Choi, I.

1983-01-01

The fundamental mechanics of delamination in fiber composite laminates is studied. Mathematical formulation of the problem is based on laminate anisotropic elasticity theory and interlaminar fracture mechanics concepts. Stress singularities and complete solution structures associated with general composite delaminations are determined. For a fully open delamination with traction-free surfaces, oscillatory stress singularities always appear, leading to physically inadmissible field solutions. A refined model is introduced by considering a partially closed delamination with crack surfaces in finite-length contact. Stress singularities associated with a partially closed delamination having frictional crack-surface contact are determined, and are found to be different from the inverse square-root one of the frictionless-contact case. In the case of a delamination with very small area of crack closure, a simplified model having a square-root stress singularity is employed by taking the limit of the partially closed delamination. The possible presence of logarithmic-type stress singularity is examined; no logarithmic singularity of any kind is found in the composite delamination problem. Numerical examples of dominant stress singularities are shown for delaminations having crack-tip closure with different frictional coefficients between general (1) and (2) graphite-epoxy composites. Previously announced in STAR as N84-13221

Instrumental record of debris flow initiation during natural rainfall: Implications for modeling slope stability

USGS Publications Warehouse

Montgomery, D.R.; Schmidt, K.M.; Dietrich, W.E.; McKean, J.

2009-01-01

The middle of a hillslope hollow in the Oregon Coast Range failed and mobilized as a debris flow during heavy rainfall in November 1996. Automated pressure transducers recorded high spatial variability of pore water pressure within the area that mobilized as a debris flow, which initiated where local upward flow from bedrock developed into overlying colluvium. Postfailure observations of the bedrock surface exposed in the debris flow scar reveal a strong spatial correspondence between elevated piezometric response and water discharging from bedrock fractures. Measurements of apparent root cohesion on the basal (Cb) and lateral (Cl) scarp demonstrate substantial local variability, with areally weighted values of Cb = 0.1 and Cl = 4.6 kPa. Using measured soil properties and basal root strength, the widely used infinite slope model, employed assuming slope parallel groundwater flow, provides a poor prediction of hydrologie conditions at failure. In contrast, a model including lateral root strength (but neglecting lateral frictional strength) gave a predicted critical value of relative soil saturation that fell within the range defined by the arithmetic and geometric mean values at the time of failure. The 3-D slope stability model CLARA-W, used with locally observed pore water pressure, predicted small areas with lower factors of safety within the overall slide mass at sites consistent with field observations of where the failure initiated. This highly variable and localized nature of small areas of high pore pressure that can trigger slope failure means, however, that substantial uncertainty appears inevitable for estimating hydrologie conditions within incipient debris flows under natural conditions. Copyright 2009 by the American Geophysical Union.

Reestablishment of endogonaceae on Mount St. Helens: survival of residuals

DOE Office of Scientific and Technical Information (OSTI.GOV)

Allen, M.F.; MacMahon, J.A.; Andersen, D.C.

1984-01-01

The 18 May 1980 eruption of Mount St. Helens resulted in the burial of relatively well developed soils under variable depths of sterile tephra and ash. During summer 1982, we examined a series of sites and estimated the numbers of spores of Endogonaceae that had been transported from the buried soil to the new ground surface by either abiotic or biotic vectors. There was no difference between spore counts of Endogone spp. or Glomus spp. in the buried soils of forests and clear-cuts; spores were rare in the tephra at any site. In areas featuring less than or equal tomore » 50 cm of tephra, spores were transported to the surface by gophers (in previous clear-cut areas) and by ants (in previous forest and clear-cut habitats). In the Pumice Plain, an area devoid of gophers and ants, erosion exposed spores to the surface. We found no evidence to suggest that endogonaceous fungi grow back up root systems from buried horizons. We hypothesize that small-scale perturbations (erosion, gopher and ant mounds) following the major volcanic disturbance may drive succession by exposing buried mycorrhizal and decomposer fungi. 26 references, 2 figures, 3 tables.« less

[Microbial Community Structure on the Root Surface of Patients with Periodontitis.

PubMed

Zhang, Ju-Mei; Zhou, Jian-Ye; Bo, Lei; Hu, Xiao-Pan; Jiao, Kang-Li; Li, Zhi-Jie; Li, Yue-Hong; Li, Zhi-Qiang

2016-11-01

To study the microbial community structure on the root surface of patients with periodontitis. Bacterial plaque and tissues from the root neck (RN group),root middle (RM group) and root tine (RT group) of six teeth with mobility 3 in one patient with periodontitis were sampled.The V3V4 region of 16S rRNA was sequenced on the Illumina MiSeq platform.The microbial community structure was analyzed by Mothur,Qiime and SPSS software. The principal component analysis (PCoA) results indicated that the RM samples had a similar microbial community structure as that of the RT samples,which was significant different from that of the RN samples.Thirteen phyla were detected in the three groups of samples,which included 7 dominant phyla.29 dominant genera were detected in 184 genera.The abundance of Bacteroidetes _[G-6] and Peptostre ptococcaceae _[XI][G-4] had a positive correlation with the depth of the collection site of samples ( P <0.05),while the abundance of Prevotella,Selenomonas,Corynebacterium and Olsenella had a negative correlation with the depth of the collection site of samples ( P <0.05). There is region-specificity of microbial community structure on the root surface of patients with periodontitis.

Mutations in Arabidopsis thaliana genes involved in the tryptophan biosynthesis pathway affect root waving on tilted agar surfaces

NASA Technical Reports Server (NTRS)

Rutherford, R.; Gallois, P.; Masson, P. H.

1998-01-01

Arabidopsis thaliana roots grow in a wavy pattern upon a slanted surface. A novel mutation in the anthranilate synthase alpha 1 (ASA1) gene, named trp5-2wvc1, and mutations in the tryptophan synthase alpha and beta 1 genes (trp3-1 and trp2-1, respectively) confer a compressed root wave phenotype on tilted agar surfaces. When trp5-2wvc1 seedlings are grown on media supplemented with anthranilate metabolites, their roots wave like wild type. Genetic and pharmacological experiments argue that the compressed root wave phenotypes of trp5-2wvc1, trp2-1 and trp3-1 seedlings are not due to reduced IAA biosynthetic potential, but rather to a deficiency in L-tryptophan (L-Trp), or in a L-Trp derivative. Although the roots of 7-day-old seedlings possess higher concentrations of free L-Trp than the shoot as a whole, trp5-2wvc1 mutants show no detectable alteration in L-Trp levels in either tissue type, suggesting that a very localized shortage of L-Trp, or of a L-Trp-derived compound, is responsible for the observed phenotype.

In vitro evaluation of the temperature increment at the external root surface after Er,Cr:YSGG laser irradiation of the root canal.

PubMed

Abad-Gallegos, M; Arnabat-Domínguez, J; España-Tost, A; Berini-Aytés, L; Gay-Escoda, C

2009-12-01

A study was made to determine the temperature increment at the dental root surface following Er,Cr:YSGG laser irradiation of the root canal. Human canines and incisors previously instrumented to K file number ISO 30 were used. Irradiation was carried out with glass fiber endodontic tips measuring 200 mm in diameter and especially designed for insertion in the root canal. The teeth were irradiated at 1 and 2 W for 30 seconds, without water spraying or air, and applying a continuous circular movement (approximately 2 mm/sec.) in the apico-coronal direction. At the 1 W power setting, the mean temperature increment was 3.84 degrees C versus 5.01 degrees C at 2 W. In all cases the difference in mean value obtained after irradiation versus the mean baseline temperature proved statistically significant (p<0.05). Application of the Er,Cr:YSGG laser gives rise to a statistically significant temperature increment at the external root surface, though this increment is probably clinically irrelevant, since it would appear to damage the tissues (periodontal ligament and alveolar bone) in proximity to the treated tooth.

Root phenotypic differences across a historical gradient of wheat genotypes alter soil rhizosphere communities and their impact on nitrogen cycling

NASA Astrophysics Data System (ADS)

Kallenbach, C.; Junaidi, D.; Fonte, S.; Byrne, P. F.; Wallenstein, M. D.

2017-12-01

Plants and soil microorganisms can exhibit coevolutionary relationships where, for example, in exchange for root carbon, rhizosphere microbes enhance plant fitness through improved plant nutrient availability. Organic agriculture relies heavily on these interactions to enhance crop nitrogen (N) availability. However, modern agriculture and breeding under high mineral N fertilization may have disrupted these interactions through alterations to belowground carbon inputs and associated impacts on the soil microbiome. As sustainability initiatives lead to a restoration of agricultural soil organic matter, modern crop cultivars may still be constrained by crop roots' ability to effectively support microbial-mediated N mineralization. We investigated how differences in root traits across a historical gradient of spring wheat genotypes influence the rhizosphere microbial community and effects on soil N and wheat yield. Five genotypes, representing wild (Wild), pre-Green Revolution (Old), and modern (Modern) wheat, were grown under greenhouse conditions in soils with and without compost to also compare genotype response to difference in native soil microbiomes and organic resource availability. We analyzed rhizosphere soils for microbial community composition, enzyme activities, inorganic N, and microbial biomass. Root length density, surface area, fine root volume and root:shoot ratio were higher in the Wild and Old genotype (Gypsum) compared to the two Modern genotypes (P<0.01). The Wild and Old genotype had a more positive response to compost for root length and diameter, N-cycling enzyme activities, microbial biomass, and soil inorganic N, compared to Modern genotypes. However, under unamended soils, the microbial community and soil N were not affected by genotypes. We also relate how root traits and N cycling across genotypes correspond to microbial community composition. Our preliminary data suggest that the older wheat genotypes and their root traits are more effective at enhancing microbial N mineralization under organically managed soils. Thus, to optimize crop N availability from organic sources, breeding efforts should consider incorporating root traits of older genotypes to better support the beneficial interactions between crop roots and their rhizosphere microbiome.

Genomic Regions Associated with Root Traits under Drought Stress in Tropical Maize (Zea mays L.)

PubMed Central

Zaidi, P. H.; Krishna, Girish; Krishnamurthy, L.; Gajanan, S.; Babu, Raman; Zerka, M.; Vinayan, M. T.; Vivek, B. S.

2016-01-01

An association mapping panel, named as CIMMYT Asia association mapping (CAAM) panel, involving 396 diverse tropical maize lines were phenotyped for various structural and functional traits of roots under drought and well-watered conditions. The experiment was conducted during Kharif (summer-rainy) season of 2012 and 2013 in root phenotyping facility at CIMMYT-Hyderabad, India. The CAAM panel was genotyped to generate 955, 690 SNPs through GBS v2.7 using Illumina Hi-seq 2000/2500 at Institute for Genomic Diversity, Cornell University, Ithaca, NY, USA. GWAS analysis was carried out using 331,390 SNPs filtered from the entire set of SNPs revealed a total of 50 and 67 SNPs significantly associated for root functional (transpiration efficiency, flowering period water use) and structural traits (rooting depth, root dry weight, root length, root volume, root surface area and root length density), respectively. In addition to this, 37 SNPs were identified for grain yield and shoot biomass under well-watered and drought stress. Though many SNPs were found to have significant association with the traits under study, SNPs that were common for more than one trait were discussed in detail. A total 18 SNPs were found to have common association with more than one trait, out of which 12 SNPs were found within or near the various gene functional regions. In this study we attempted to identify the trait specific maize lines based on the presence of favorable alleles for the SNPs associated with multiple traits. Two SNPs S3_128533512 and S7_151238865 were associated with transpiration efficiency, shoot biomass and grain yield under well-watered condition. Based on favorable allele for these SNPs seven inbred lines were identified. Similarly, four lines were identified for transpiration efficiency and shoot biomass under drought stress based on the presence of favorable allele for the common SNPs S1_211520521, S2_20017716, S3_57210184 and S7_130878458 and three lines were identified for flowering period water-use, transpiration efficiency, root dry weight and root volume based on the presence of favorable allele for the common SNPs S3_162065732 and S3_225760139. PMID:27768702

Artificial Root Exudate System (ARES): a field approach to simulate tree root exudation in soils

NASA Astrophysics Data System (ADS)

Lopez-Sangil, Luis; Estradera-Gumbau, Eduard; George, Charles; Sayer, Emma

2016-04-01

The exudation of labile solutes by fine roots represents an important strategy for plants to promote soil nutrient availability in terrestrial ecosystems. Compounds exuded by roots (mainly sugars, carboxylic and amino acids) provide energy to soil microbes, thus priming the mineralization of soil organic matter (SOM) and the consequent release of inorganic nutrients into the rhizosphere. Studies in several forest ecosystems suggest that tree root exudates represent 1 to 10% of the total photoassimilated C, with exudation rates increasing markedly under elevated CO2 scenarios. Despite their importance in ecosystem functioning, we know little about how tree root exudation affect soil carbon dynamics in situ. This is mainly because there has been no viable method to experimentally control inputs of root exudates at field scale. Here, I present a method to apply artificial root exudates below the soil surface in small field plots. The artificial root exudate system (ARES) consists of a water container with a mixture of labile carbon solutes (mimicking tree root exudate rates and composition), which feeds a system of drip-tips covering an area of 1 m2. The tips are evenly distributed every 20 cm and inserted 4-cm into the soil with minimal disturbance. The system is regulated by a mechanical timer, such that artificial root exudate solution can be applied at frequent, regular daily intervals. We tested ARES from April to September 2015 (growing season) within a leaf-litter manipulation experiment ongoing in temperate deciduous woodland in the UK. Soil respiration was measured monthly, and soil samples were taken at the end of the growing season for PLFA, enzymatic activity and nutrient analyses. First results show a very rapid mineralization of the root exudate compounds and, interestingly, long-term increases in SOM respiration, with negligible effects on soil moisture levels. Large positive priming effects (2.5-fold increase in soil respiration during the growing season) were observed in absence of aboveground forest litter, with lower or no priming when the litter was present. Preliminary results show that soil microbial community is also significantly affected by ARES.

Influence of surface roughness on cetyltrimethylammonium bromide adsorption from aqueous solution.

PubMed

Wu, Shuqing; Shi, Liu; Garfield, Lucas B; Tabor, Rico F; Striolo, Alberto; Grady, Brian P

2011-05-17

The influence of surface roughness on surfactant adsorption was studied using a quartz crystal microbalance with dissipation (QCM-D). The sensors employed had root-mean-square (R) roughness values of 2.3, 3.1, and 5.8 nm, corresponding to fractal-calculated surface area ratios (actual/nominal) of 1.13, 1.73, and 2.53, respectively. Adsorption isotherms measured at 25 °C showed that adsorbed mass of cetyltrimethylammonium bromide per unit of actual surface area below 0.8 cmc, or above 1.2 cmc, decreases as the surface roughness increases. At the cmc, both the measured adsorbed amount and the measured dissipation increased dramatically on the rougher surfaces. These results are consistent with the presence of impurities, suggesting that roughness exacerbates well-known phenomena reported in the literature of peak impurity-related adsorption at the cmc. The magnitude of the increase, especially in dissipation, suggests that changes in adsorbed amount may not be the only reason for the observed results, as aggregates at the cmc on rougher surfaces are more flexible and likely contain larger amounts of solvent. Differences in adsorption kinetics were also found as a function of surface roughness, with data showing a second, slower adsorption rate after rapid initial adsorption. A two-rate Langmuir model was used to further examine this effect. Although adsorption completes faster on the smoother surfaces, initial adsorption at zero surface coverage is faster on the rougher surfaces, suggesting the presence of more high-energy sites on the rougher surfaces.

Prediction of Root Zone Soil Moisture using Remote Sensing Products and In-Situ Observation under Climate Change Scenario

NASA Astrophysics Data System (ADS)

Singh, G.; Panda, R. K.; Mohanty, B.

2015-12-01

Prediction of root zone soil moisture status at field level is vital for developing efficient agricultural water management schemes. In this study, root zone soil moisture was estimated across the Rana watershed in Eastern India, by assimilation of near-surface soil moisture estimate from SMOS satellite into a physically-based Soil-Water-Atmosphere-Plant (SWAP) model. An ensemble Kalman filter (EnKF) technique coupled with SWAP model was used for assimilating the satellite soil moisture observation at different spatial scales. The universal triangle concept and artificial intelligence techniques were applied to disaggregate the SMOS satellite monitored near-surface soil moisture at a 40 km resolution to finer scale (1 km resolution), using higher spatial resolution of MODIS derived vegetation indices (NDVI) and land surface temperature (Ts). The disaggregated surface soil moisture were compared to ground-based measurements in diverse landscape using portable impedance probe and gravimetric samples. Simulated root zone soil moisture were compared with continuous soil moisture profile measurements at three monitoring stations. In addition, the impact of projected climate change on root zone soil moisture were also evaluated. The climate change projections of rainfall were analyzed for the Rana watershed from statistically downscaled Global Circulation Models (GCMs). The long-term root zone soil moisture dynamics were estimated by including a rainfall generator of likely scenarios. The predicted long term root zone soil moisture status at finer scale can help in developing efficient agricultural water management schemes to increase crop production, which lead to enhance the water use efficiency.

Flooding Hazard Maps of Different Land Uses in Subsidence Area

NASA Astrophysics Data System (ADS)

Lin, Yongjun; Chang, Hsiangkuan; Tan, Yihchi

2017-04-01

This study aims on flooding hazard maps of different land uses in the subsidence area of southern Taiwan. Those areas are low-lying due to subsidence resulting from over pumping ground water for aquaculture. As a result, the flooding due to storm surges and extreme rainfall are frequent in this area and are expected more frequently in the future. The main land uses there include: residence, fruit trees, and aquaculture. The hazard maps of the three land uses are investigated. The factors affecting hazards of different land uses are listed below. As for residence, flooding depth, duration of flooding, and rising rate of water surface level are factors affecting its degree of hazard. High flooding depth, long duration of flooding, and fast rising rate of water surface make residents harder to evacuate. As for fruit trees, flooding depth and duration of flooding affects its hazard most due to the root hypoxia. As for aquaculture, flooding depth affects its hazard most because the high flooding depth may cause the fish flush out the fishing ponds. An overland flow model is used for simulations of hydraulic parameters for factors such as flooding depth, rising rate of water surface level and duration of flooding. As above-mentioned factors, the hazard maps of different land uses can be made and high hazardous are can also be delineated in the subsidence areas.

Binding of isolated plant lectin by rhizobia during episodes of reduced gravity obtained by parabolic flight

NASA Technical Reports Server (NTRS)

Henry, R. L.; Green, P. D.; Wong, P. P.; Guikema, J. A.; Spooner, B. S. (Principal Investigator)

1990-01-01

Development of a legume root nodule is a complex process culminating in a plant/bacterial symbiosis possessing the capacity for biological dinitrogen fixation. Formation of root nodules is initiated by the binding and stabilization of rhizobia to plant root hairs, mediated in part by a receptor/ligand recognition system composed of lectins on the plant root surface and lectin-binding sites on the rhizobial cell surface. The dinitrogen fixation activity of these root nodules may be an important feature of enclosed, space-based life support systems, and may provide an ecological method to recycle nitrogen for amino acid production. However, the effects on nodule development of varied gravitational fields, or of root nutrient delivery hardware, remain unknown. We have investigated the effects of microgravity on root nodule formation, with preliminary experiments focused upon the receptor/ligand component. Microgravity, obtained during parabolic flight aboard NASA 930, has no apparent effect on the binding of purified lectin to rhizobia, a result that will facilitate forthcoming experiments using intact root tissues.

Stand age and fine root biomass, distribution and morphology in a Norway spruce chronosequence in southeast Norway.

PubMed

Børja, Isabella; De Wit, Heleen A; Steffenrem, Arne; Majdi, Hooshang

2008-05-01

We assessed the influence of stand age on fine root biomass and morphology of trees and understory vegetation in 10-, 30-, 60- and 120-year-old Norway spruce stands growing in sandy soil in southeast Norway. Fine root (< 1, 1-2 and 2-5 mm in diameter) biomass of trees and understory vegetation (< 2 mm in diameter) was sampled by soil coring to a depth of 60 cm. Fine root morphological characteristics, such as specific root length (SRL), root length density (RLD), root surface area (RSA), root tip number and branching frequency (per unit root length or mass), were determined based on digitized root data. Fine root biomass and morphological characteristics related to biomass (RLD and RSA) followed the same tendency with chronosequence and were significantly higher in the 30-year-old stand and lower in the 10-year-old stand than in the other stands. Among stands, mean fine root (< 2 mm) biomass ranged from 49 to 398 g m(-2), SLR from 13.4 to 19.8 m g(-1), RLD from 980 to 11,650 m m(-3) and RSA from 2.4 to 35.4 m(2) m(-3). Most fine root biomass of trees was concentrated in the upper 20 cm of the mineral soil and in the humus layer (0-5 cm) in all stands. Understory fine roots accounted for 67 and 25% of total fine root biomass in the 10- and 120-year-old stands, respectively. Stand age had no affect on root tip number or branching frequency, but both parameters changed with soil depth, with increasing number of root tips and decreasing branching frequency with increasing soil depth for root fractions < 2 mm in diameter. Specific (mass based) root tip number and branching density were highest for the finest roots (< 1 mm) in the humus layer. Season (spring or fall) had no effect on tree fine root biomass, but there was a small and significant increase in understory fine root biomass in fall relative to spring. All morphological characteristics showed strong seasonal variation, especially the finest root fraction, with consistently and significantly higher values in spring than in fall. We conclude that fine root biomass, especially in the finest fraction (< 1 mm in diameter), is strongly dependent on stand age. Among stands, carbon concentration in fine root biomass was highest in the 30-year-old stand, and appeared to be associated with the high tree and canopy density during the early stage of stand development. Values of RLD and RSA, morphological features indicative of stand nutrient-uptake efficiency, were higher in the 30-year-old stand than in the other stands.

Root elongation against a constant force: experiment with a computerized feedback-controlled device

NASA Technical Reports Server (NTRS)

Kuzeja, P. S.; Lintilhac, P. M.; Wei, C.

2001-01-01

Axial force was applied to the root tip of corn (Zea mays L. cv. Merit) seedlings using a computerized, feedback-controlled mechanical device. The system's feedback capability allowed continuous control of a constant tip load, and the attached displacement transducer provided the time course of root elongation. Loads up to 7.5 g decreased the root elongation rate by 0.13 mm h-1 g-1, but loads 7.5 to 17.5 g decreased the growth rate by only 0.04 mm h-1 g-1. Loads higher than 18 g stopped root elongation completely. Measurement of the cross-sectional areas of the root tips indicated that the 18 g load had applied about 0.98 MPa of axial pressure to the root, thereby exceeding the root's ability to respond with increased turgor pressure. Recorded time-lapse images of loaded roots showed that radial thickening (swelling) occurred behind the root cap, whose cross-sectional area increased with tip load.

Effects of optimized root water uptake parameterization schemes on water and heat flux simulation in a maize agroecosystem

NASA Astrophysics Data System (ADS)

Cai, Fu; Ming, Huiqing; Mi, Na; Xie, Yanbing; Zhang, Yushu; Li, Rongping

2017-04-01

As root water uptake (RWU) is an important link in the water and heat exchange between plants and ambient air, improving its parameterization is key to enhancing the performance of land surface model simulations. Although different types of RWU functions have been adopted in land surface models, there is no evidence as to which scheme most applicable to maize farmland ecosystems. Based on the 2007-09 data collected at the farmland ecosystem field station in Jinzhou, the RWU function in the Common Land Model (CoLM) was optimized with scheme options in light of factors determining whether roots absorb water from a certain soil layer ( W x ) and whether the baseline cumulative root efficiency required for maximum plant transpiration ( W c ) is reached. The sensibility of the parameters of the optimization scheme was investigated, and then the effects of the optimized RWU function on water and heat flux simulation were evaluated. The results indicate that the model simulation was not sensitive to W x but was significantly impacted by W c . With the original model, soil humidity was somewhat underestimated for precipitation-free days; soil temperature was simulated with obvious interannual and seasonal differences and remarkable underestimations for the maize late-growth stage; and sensible and latent heat fluxes were overestimated and underestimated, respectively, for years with relatively less precipitation, and both were simulated with high accuracy for years with relatively more precipitation. The optimized RWU process resulted in a significant improvement of CoLM's performance in simulating soil humidity, temperature, sensible heat, and latent heat, for dry years. In conclusion, the optimized RWU scheme available for the CoLM model is applicable to the simulation of water and heat flux for maize farmland ecosystems in arid areas.

An In-Situ Root-Imaging System in the Context of Surface Detection of CO2

NASA Astrophysics Data System (ADS)

Apple, M. E.; Prince, J. B.; Bradley, A. R.; Zhou, X.; Lakkaraju, V. R.; Male, E. J.; Pickles, W.; Thordsen, J. J.; Dobeck, L.; Cunningham, A.; Spangler, L.

2009-12-01

Carbon sequestration is a valuable method of spatially confining CO2 belowground. The Zero Emissions Research Technology, (ZERT), site is an experimental facility in a former agricultural field on the Montana State University campus in Bozeman, Montana, where CO2 was experimentally released at a rate of 200kg/day in 2009 into a 100 meter underground injection well running parallel to the ground surface. This injection well, or pipe, has deliberate leaks at intervals, and CO2 travels from these leaks upward to the surface of the ground. The ZERT site is a model system designed with the purpose of testing methods of surface detection of CO2. One important aspect of surface detection is the determination of the effects of CO2 on the above and belowground portions of plants growing above sequestration fields. At ZERT, these plants consist of a pre-existing mixture of herbaceous species present at the agricultural field. Species growing at the ZERT site include several grasses, Dactylis glomerata (Orchard Grass), Poa pratensis (Kentucky Bluegrass), and Bromus japonicus (Japanese Brome); the nitrogen-fixing legumes Medicago sativa, (Alfalfa), and Lotus corniculatus, (Birdsfoot trefoil); and an abundance of Taraxacum officinale, (Dandelion). Although the aboveground parts of the plants at high CO2 are stressed, as indicated by changes in hyperspectral plant signatures, leaf fluorescence and leaf chlorophyll content, we are interested in determining whether the roots are also stressed. To do so, we are combining measurements of soil conductivity and soil moisture with root imaging. We are using an in-situ root-imaging system manufactured by CID, Inc. (Camas, WA), along with image analysis software (Image-J) to analyze morphometric parameters in the images and to determine what effects, if any, the presence of leaking and subsequently upwelling CO2 has on the phenology of root growth, growth and turnover of individual fine and coarse roots, branching patterns, and root density and depth in the soil. We drilled three holes for the plexiglass root-imaging tubes in December 2008 and installed the tubes post-thaw in May 2009, with the initial set of images taken in July 2009 on the day preceding the 4-week long CO2 injection. We collected images weekly thereafter until late August 2009 by inserted a rotating camera into the tube and photographing at 10 cm intervals from the surface to a depth of 75-80 cm. By August 2009, roots were visible at 80 cm below ground. The root-imaging tubes will remain in place so that we can track the roots through the upcoming years at the ZERT site. Each year, we anticipate gathering images in the fall, winter, before the beginning of root growth in the spring, as well as during the summer injections of CO2. The information gained from these images will be useful in linking above and belowground responses of plants to CO2.

KSC-05pd2263

NASA Image and Video Library

2005-09-28

KENNEDY SPACE CENTER, FLA. - Two wild pigs cross railroad tracks on grounds at NASA Kennedy Space Center. The wild pigs have flourished in the environs around KSC, which shares a border with the Merritt Island National Wildlife Refuge, without many predators other than panthers and humans. Pigs were introduced to Florida in the 1500s and are now found statewide in wooded areas close to water. Pigs are omnivores, foraging on the ground and rooting just beneath the surface, which damages the groundcover. Wild pigs eat almost anything that has nutritional value, including tubers, roots, shoots, acorns, fruits, berries, earthworms, amphibians, reptiles and rodents. Appearance is similar to domestic hogs, but leaner, with a longer, narrower head and a coarser, denser coat. Females may have two litters per year. The piglets are weaned in a few weeks but remain with the mother for several months.

Differential response of hexaploid and tetraploid wheat to interactive effects of elevated [CO2] and low phosphorus.

PubMed

Pandey, Renu; Lal, Milan Kumar; Vengavasi, Krishnapriya

2018-06-04

Hexaploid wheat is more responsive than tetraploid to the interactive effects of elevated [CO 2 ] and low P in terms of carboxylate efflux, enzyme activity and gene expression (TaPT1 and TaPAP). Availability of mineral nutrients to plants under changing climate has become a serious challenge to food security and economic development. An understanding of how elevated [CO 2 ] influences phosphorus (P) acquisition processes at the whole-plant level would be critical in selecting cultivars as well as to maintain optimum yield in limited-P conditions. Wheat (Triticum aestivum and T. durum) grown hydroponically with sufficient and low P concentration were exposed to elevated and ambient [CO 2 ]. Improved dry matter partitioning towards root resulted in increased root-to-shoot ratio, root length, volume, surface area, root hair length and density at elevated [CO 2 ] with low P. Interaction of low P and [CO 2 ] induced activity of enzymes (phosphoenolpyruvate carboxylase, malate dehydrogenase and citrate synthase) in root tissue resulting in twofold increase in carboxylates and acid phosphatase exudation. Physiological absorption capacity of roots showed that plants alter their uptake kinetics by increasing affinity (low K m ) in response to elevated [CO 2 ] under low P supply. Increased relative expression of genes, purple acid phosphatase (TaPAP) and high-affinity Pi transporter (TaPT1) in roots induced by elevated [CO 2 ] and low P supported our physiological observations. Hexaploid wheat (PBW-396) being more responsive to elevated [CO 2 ] at low P supply as compared to tetraploid (PDW-233) necessitates the ploidy effect to be explored further which might be advantageous under changing climate.

Histological changes induced by 15 F CO2 laser microprobe especially designed for root canal sterilization: an in-vivo study

NASA Astrophysics Data System (ADS)

Kesler, Gavriel; Koren, Rumelia; Gal, Rivka

1998-04-01

Until now, no suitable delivery fiber existed for CO2 laser endodontic radiation in the apical region where it is most difficult to eliminate the pulp tissue using conventional methods. To overcome this problem, we designed a microprobe that reaches closer to the apex, distributing the energy density to a smaller area of the root canal, thus favorably increasing the thermal effects. The 15 F CO2 microprobe is a flexible, hollow, metal fiber, 300 micrometer in diameter and 20 mm in length, coupled onto a handpiece, with the following radiation parameters: wavelength -- 10.6 micrometer; pulse duration -- 50m/sec; energy per pulse 0.25 joule; energy density -- 353.7J/cm2 per pulse; power on tissue -- 5 W. The study was conducted on 30 vital maxillary or mandibulary; central, lateral, or premolar teeth destined for extraction due to periodontal problems. Twenty were experimentally treated with pulsed CO2 laser delivered by this newly developed fiber after conventional root canal preparation. Temperature measured at three points on the root surface during laser treatment did not exceed 38 degrees Celsius. Ten teeth represented the control group in which only root canal preparation was performed in the conventional method. Histological examination of the laser treated teeth showed coagulation necrosis and vacuolization of remaining pulp tissue in the root canal periphery. Primary and secondary dentin appeared normal, in all cases treated with 15 F CO2 laser. Gramm stain and bacteriologic examination revealed complete sterilization. These results demonstrate the unique capabilities of this special microprobe in sterilization of the root canal, and no thermal damage to the surrounding tissue.

Sub-epithelial connective tissue graft for root coverage in nonsmokers and smokers: A pilot comparative clinical study

PubMed Central

Dwarakanath, Chini Doraswamy; Divya, Bheemavarapu; Sruthima, Gottumukkala Naga Venkata Satya; Penmetsa, Gautami Subadra

2016-01-01

Background: Gingival recession is a common condition and is more prevalent in smokers. It is widely believed that root coverage procedures in smokers result in less desirable outcome compared to nonsmokers', and there are few controlled studies in literature to support this finding. Therefore, the purpose of this study was to evaluate and compare the outcome of root coverage with sub-epithelial connective tissue graft (SCTG) in nonsmokers and smokers. Materials and Methods: A sample of twenty subjects, 10 nonsmokers and 10 smokers were selected each with at least 1 Miller's Class I or II recession on a single rooted tooth. Clinical measurements of probing depth, clinical attachment level (CAL), gingival recession total surface area (GRTSA), depth of recession (RD), width of recession (RW), and width of keratinized tissue were determined at baseline, 3, and 6 months after surgery. Results: The treatment of gingival recession with SCTG and coronally advanced flap showed a decrease in the GRTSA, RD, RW, and an increase in CAL and width of keratinized gingiva in both the groups. However, the intergroup comparison of the clinical parameters showed no statistical significance. About 6 out of 10 nonsmokers (60%) and 3 smokers (30%) showed complete root coverage. The mean percentage of root coverage of 71.2% in nonsmokers and 38% in smokers was observed. Conclusion: The results of the present study suggest that smoking may negatively influence gingival recession reduction and CAL gain. In addition, smokers may exhibit fewer chances of complete root coverage. Overall, nonsmokers showed better improvements in all the parameters compared to smokers at the end of 6 months. PMID:28298827

Pyridine 2,4-dicarboxylic acid suppresses tomato seedling growth

NASA Astrophysics Data System (ADS)

Fragkostefanakis, Sotirios; Kaloudas, Dimitrios; Kalaitzis, Panagiotis

2018-01-01

Pyridine 2,4-dicarboxylic acid is a structural analogue of 2-oxoglutarate and is known to inhibit 2-oxoglutare-dependent dioxygenases. The effect of this inhibitor in tomato seedlings grown in MS media supplied with various concentrations of PDCA was investigated, resulting in shorter roots and hypocotyls in a dose-dependent manner. The partial inhibition of growth in roots was more drastic compared to hypocotyls and was attributed to a decrease in the elongation of root and hypocotyl cells. Concentrations of 100 and 250 μΜ of PDCA decreased hydroxyproline content in roots while only the 250 μΜ treatment reduced the hydroxyproline content in shoots. Seedlings treated with 100 μΜ PDCA exhibited enhanced growth of hypocotyl and cotyledon cells and higher hydroxyproline content resulting in cotyledons with greater surface area. However, no alterations in hypocotyl length were observed. Prolyl 4 hydroxylases (P4Hs) are involved in the O-glycosylation of AGPs and were also highly expressed during seedling growth. Moreover PDCA induced a decrease in the accumulation of HRGPs and particularly in AGPs-bound epitopes in a dose dependent-manner while more drastic reduction were observed in roots compared to shoots. In addition, bulged root epidermal cells were observed at the high concentration of 250 μΜ which is characteristic of root tissues with glycosylation defects. These results indicate that PDCA induced pleiotropic effects during seedling growth while further studies are required to better investigate the physiological significance of this 2-oxoglutarate analogue. This pharmacological approach might be used as a tool to better understand the physiological significance of HRGPs and probably P4Hs in various growth and developmental programs in plants.

Soil Nitrogen Status Modifies Rice Root Response to Nematode-Bacteria Interactions in the Rhizosphere

PubMed Central

Wu, Yue; Valentine, Tracy A.; Li, Huixin

2016-01-01

It has been hypothesized that faunal activity in the rhizosphere influences root growth via an auxin-dependent pathway. In this study, two methods were used to adjust nematode and bacterial populations within experimental soils. One is “exclusion”, where soil mixed with pig manure was placed in two bags with different mesh sizes (1mm and 5μm diameter), and then surrounded by an outer layer of unamended soil resulting in soil with a greater populations of bacterial-feeding nematodes (1mm) and a control treatment (5μm). The second method is “inoculation”, whereby autoclaved soil was inoculated with bacteria (E. coli and Pseudomonas) and Nematodes (Cephalobus and C. elegans). In order to detect the changes in the rice’s perception of auxin under different nutrient and auxin conditions in the presence of soil bacterial-feeding nematodes, responses of soil chemistry (NH4+, NO3- and indole acetic acid (IAA)), rice root growth and the expression of an auxin responsive gene GH3-2 were measured. Results showed that, under low soil nutrient conditions (exclusion), low NO3- correlated with increased root branching and IAA correlated with increased root elongation and GH3-2 expression. However, under high soil nutrient conditions (inoculation), a high NH4+ to NO3- ratio promoted an increase in root surface area and there was an additional influence of NH4+ and NO3- on GH3-2 expression. Thus it was concluded that soil bacterial-feeding nematodes influenced soil nutritional status and soil IAA content, promoting root growth via an auxin dependent pathway that was offset by soil nitrogen status. PMID:26841062

Effect of the fungus Piriformospora indica on physiological characteristics and root morphology of wheat under combined drought and mechanical stresses.

PubMed

Hosseini, Fatemeh; Mosaddeghi, Mohammad Reza; Dexter, Anthony Roger

2017-09-01

This study was done to evaluate the effects of the root-colonizing endophytic fungus Piriformospora indica on wheat growth under combined drought and mechanical stresses. Inoculated (colonized) and non-inoculated (uncolonized) wheat (Triticum aestivum L. cv. Chamran) seedlings were planted in growth chambers filled with moist sand (at a matric suction of 20 hPa). Slight, moderate and severe mechanical stresses (i.e., penetration resistance, Q p , of 1.17, 4.17 and 5.96 MPa, respectively) were produced by a dead-load technique (i.e., placing a weight on the sand surface) in the root medium. Slight, moderate and severe drought stresses were induced using PEG 6000 solutions with osmotic potentials of 0, -0.3 and -0.5 MPa, respectively. After 30 days, plant physiological characteristics and root morphology were measured. An increase in Q p from 1.17 to 5.96 MPa led to greater leaf proline concentration and root diameter, and lower relative water content (RWC), leaf water potential (LWP), chlorophyll contents and root volume. Moreover, severe drought stress decreased root and shoot fresh weights, root volume, leaf area, RWC, LWP and chlorophyll content compared to control. Catalase (CAT) and ascorbate peroxidase (APX) activities under severe drought stress were about 1.5 and 2.9 times greater than control. Interaction of the stresses showed that mechanical stress primarily controls plant water status and physiological responses. However, endophyte presence mitigated the adverse effects of individual and combined stresses on plant growth. Colonized plants were better adapted and had greater root length and volume, RWC, LWP and chlorophyll contents under stressful conditions due to higher absorption sites for water and nutrients. Compared with uncolonized plants, colonized plants showed lower CAT activity implying that wheat inoculated with P. indica was more tolerant and experienced less oxidative damage induced by drought and/or mechanical stress. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Uptake, translocation and transformation of antimony in rice (Oryza sativa L.) seedlings.

PubMed

Cai, Fei; Ren, Jinghua; Tao, Shu; Wang, Xilong

2016-02-01

Antimony (Sb), as a toxic metalloid, has been gaining increasing research concerns due mainly to its severe pollution in many places. Rice has been identified to be the dominant intake route of Sb by residents close to the Sb mining areas. A hydroponic experiment was conducted to investigate the difference in uptake, translocation and transformation of Sb in rice seedlings of four cultivars exposed to 0.2 or 1.0 mg/L of Sb(V). The results showed that mass concentration of iron plaque (mg/kg FW) formed at the root surfaces of cultivar N was the highest among all tested cultivars at both low and high exposure levels of Sb(V). The accumulated Sb concentration in iron plaque significantly increased with an increase in mass concentration of iron plaque formed at the rice root. The total amount of iron plaque (mg/pot) at rice root generally increased with increasing exposed Sb(V) concentration, which was closely associated with the increasing lipid peroxidation in roots. Concentration percentage of Sb in rice root significantly reduced as the corresponding value in the iron plaque increased, suggesting that iron plaque formation strongly suppressed uptake of Sb by rice root. Sb concentration in rice tissues followed an order: root > stem, leaf. The japonica rice (cultivars N and Z) exhibited a stronger translocation tendency of Sb from root to stem than indica hybrid rice (cultivars F and G). Translocation of Sb from root of cultivar F to its stem and leaf was sharply enhanced with increasing Sb exposure concentration. Sb(V) could be reduced to Sb(III) in rice tissues, especially in stems (10-26% of the total Sb). For the sake of food safety, the difference in uptake, translocation and transformation of Sb in rice species planted in Sb-contaminated soils should be taken into consideration. Copyright © 2015 Elsevier Ltd. All rights reserved.

Fine root morphological adaptations in Scots pine, Norway spruce and silver birch along a latitudinal gradient in boreal forests.

PubMed

Ostonen, Ivika; Lõhmus, Krista; Helmisaari, Heljä-Sisko; Truu, Jaak; Meel, Signe

2007-11-01

Variability in short root morphology of the three main tree species of Europe's boreal forest (Norway spruce (Picea abies L. Karst.), Scots pine (Pinus sylvestris L.) and silver birch (Betula pendula Roth)) was investigated in four stands along a latitudinal gradient from northern Finland to southern Estonia. Silver birch and Scots pine were present in three stands and Norway spruce was present in all stands. For three fertile Norway spruce stands, fine root biomass and number of root tips per stand area or unit basal area were assessed from north to south. Principal component analysis indicated that short root morphology was significantly affected by tree species and site, which together explained 34.7% of the total variability. The range of variation in mean specific root area (SRA) was 51-74, 60-70 and 84-124 m(2) kg(-1) for Norway spruce, Scots pine and silver birch, respectively, and the corresponding ranges for specific root length were 37-47, 40-48 and 87-97 m g(-1). The range of variation in root tissue density of Norway spruce, Scots pine and silver birch was 113-182, 127-158 and 81-156 kg m(-3), respectively. Sensitivity of short root morphology to site conditions decreased in the order: Norway spruce > silver birch > Scots pine. Short root SRA increased with site fertility in all species. In Norway spruce, fine root biomass and number of root tips per m(2) decreased from north to south. The differences in morphological parameters among sites were significant but smaller than the site differences in fine root biomass and number of root tips.

Antibacterial Nanoparticles in Endodontics: A Review.

PubMed

Shrestha, Annie; Kishen, Anil

2016-10-01

A major challenge in root canal treatment is the inability of the current cleaning and shaping procedures to eliminate bacterial biofilms surviving within the anatomic complexities and uninstrumented portions of the root canal system. Nanoparticles with their enhanced and unique physicochemical properties, such as ultrasmall sizes, large surface area/mass ratio, and increased chemical reactivity, have led research toward new prospects of treating and preventing dental infections. This article presents a comprehensive review on the scientific knowledge that is available on the application of antibacterial nanoparticles in endodontics. The application of nanoparticles in the form of solutions for irrigation, medication, and as an additive within sealers/restorative materials has been evaluated to primarily improve the antibiofilm efficacy in root canal and restorative treatments. In addition, antibiotic or photosensitizer functionalized nanoparticles have been proposed recently to provide more potent antibacterial efficacy. The increasing interest in this field warrants sound research based on scientific and clinical collaborations to emphasize the near future potential of nanoparticles in clinical endodontics. Copyright © 2016 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

A representation of the phosphorus cycle for ORCHIDEE (revision 4520)

NASA Astrophysics Data System (ADS)

Goll, Daniel S.; Vuichard, Nicolas; Maignan, Fabienne; Jornet-Puig, Albert; Sardans, Jordi; Violette, Aurelie; Peng, Shushi; Sun, Yan; Kvakic, Marko; Guimberteau, Matthieu; Guenet, Bertrand; Zaehle, Soenke; Penuelas, Josep; Janssens, Ivan; Ciais, Philippe

2017-10-01

Land surface models rarely incorporate the terrestrial phosphorus cycle and its interactions with the carbon cycle, despite the extensive scientific debate about the importance of nitrogen and phosphorus supply for future land carbon uptake. We describe a representation of the terrestrial phosphorus cycle for the ORCHIDEE land surface model, and evaluate it with data from nutrient manipulation experiments along a soil formation chronosequence in Hawaii. ORCHIDEE accounts for the influence of the nutritional state of vegetation on tissue nutrient concentrations, photosynthesis, plant growth, biomass allocation, biochemical (phosphatase-mediated) mineralization, and biological nitrogen fixation. Changes in the nutrient content (quality) of litter affect the carbon use efficiency of decomposition and in return the nutrient availability to vegetation. The model explicitly accounts for root zone depletion of phosphorus as a function of root phosphorus uptake and phosphorus transport from the soil to the root surface. The model captures the observed differences in the foliage stoichiometry of vegetation between an early (300-year) and a late (4.1 Myr) stage of soil development. The contrasting sensitivities of net primary productivity to the addition of either nitrogen, phosphorus, or both among sites are in general reproduced by the model. As observed, the model simulates a preferential stimulation of leaf level productivity when nitrogen stress is alleviated, while leaf level productivity and leaf area index are stimulated equally when phosphorus stress is alleviated. The nutrient use efficiencies in the model are lower than observed primarily due to biases in the nutrient content and turnover of woody biomass. We conclude that ORCHIDEE is able to reproduce the shift from nitrogen to phosphorus limited net primary productivity along the soil development chronosequence, as well as the contrasting responses of net primary productivity to nutrient addition.

A Novel Method for Characterizing Beam Hardening Artifacts in Cone-beam Computed Tomographic Images.

PubMed

Fox, Aaron; Basrani, Bettina; Kishen, Anil; Lam, Ernest W N

2018-05-01

The beam hardening (BH) artifact produced by root filling materials in cone-beam computed tomographic (CBCT) images is influenced by their radiologic K absorption edge values. The purpose of this study was to describe a novel technique to characterize BH artifacts in CBCT images produced by 3 root canal filling materials and to evaluate the effects of a zirconium (Zr)-based root filling material with a lower K edge (17.99 keV) on the production of BH artifacts. The palatal root canals of 3 phantom model teeth were prepared and root filled with gutta-percha (GP), a Zr root filling material, and calcium hydroxide paste. Each phantom tooth was individually imaged using the CS 9000 CBCT unit (Carestream, Atlanta, GA). The "light" and "dark" components of the BH artifacts were quantified separately using ImageJ software (National Institutes of Health, Bethesda, MD) in 3 regions of the root. Mixed-design analysis of variance was used to evaluate differences in the artifact area for the light and dark elements of the BH artifacts. A statistically significant difference in the area of the dark portion of the BH artifact was found between all fill materials and in all regions of the phantom tooth root (P < .05). GP generated a significantly greater dark but not light artifact area compared with Zr (P < .05). Moreover, statistically significant differences between the areas of both the light and dark artifacts were observed within all regions of the tooth root, with the greatest artifact being generated in the coronal third of the root (P < .001). Root canal filling materials with lower K edge material properties reduce BH artifacts along the entire length of the root canal and reduce the contribution of the dark artifact. Copyright © 2018 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Effects of 24-epibrassinolide on enzymatic browning and antioxidant activity of fresh-cut lotus root slices.

PubMed

Gao, Hui; Chai, HongKang; Cheng, Ni; Cao, Wei

2017-02-15

Fresh-cut lotus root slices were treated with 80nM 24-epibrassinolide (EBR) and then stored at 4°C for 8days to investigate the effects on cut surface browning. The results showed that EBR treatment reduced cut surface browning in lotus root slices and alleviated membrane lipid peroxidation as reflected by low malondialdehyde content and lipoxygenase activity. EBR treatment inhibited the activity of phenylalanine ammonia lyase and polyphenol oxidase, and subsequently decreased phenolics accumulation and soluble quniones formation. The treatment also stimulated the activity of peroxidase, catalase and ascorbate peroxidase and delayed the loss of ascorbic acid, which would help prevent membrane lipid peroxidation, as a consequence, reducing decompartmentation of enzymes and substrates causing enzymatic browning. These results indicate that EBR treatment is a promising attempt to control browning at cut surface of fresh-cut lotus root slices. Copyright © 2016 Elsevier Ltd. All rights reserved.

Plant roots use a patterning mechanism to position lateral root branches toward available water.

PubMed

Bao, Yun; Aggarwal, Pooja; Robbins, Neil E; Sturrock, Craig J; Thompson, Mark C; Tan, Han Qi; Tham, Cliff; Duan, Lina; Rodriguez, Pedro L; Vernoux, Teva; Mooney, Sacha J; Bennett, Malcolm J; Dinneny, José R

2014-06-24

The architecture of the branched root system of plants is a major determinant of vigor. Water availability is known to impact root physiology and growth; however, the spatial scale at which this stimulus influences root architecture is poorly understood. Here we reveal that differences in the availability of water across the circumferential axis of the root create spatial cues that determine the position of lateral root branches. We show that roots of several plant species can distinguish between a wet surface and air environments and that this also impacts the patterning of root hairs, anthocyanins, and aerenchyma in a phenomenon we describe as hydropatterning. This environmental response is distinct from a touch response and requires available water to induce lateral roots along a contacted surface. X-ray microscale computed tomography and 3D reconstruction of soil-grown root systems demonstrate that such responses also occur under physiologically relevant conditions. Using early-stage lateral root markers, we show that hydropatterning acts before the initiation stage and likely determines the circumferential position at which lateral root founder cells are specified. Hydropatterning is independent of endogenous abscisic acid signaling, distinguishing it from a classic water-stress response. Higher water availability induces the biosynthesis and transport of the lateral root-inductive signal auxin through local regulation of tryptophan aminotransferase of Arabidopsis 1 and PIN-formed 3, both of which are necessary for normal hydropatterning. Our work suggests that water availability is sensed and interpreted at the suborgan level and locally patterns a wide variety of developmental processes in the root.

Biomechanical consequences of a complete radial tear adjacent to the medial meniscus posterior root attachment site: in situ pull-out repair restores derangement of joint mechanics.

PubMed

Padalecki, Jeffrey R; Jansson, Kyle S; Smith, Sean D; Dornan, Grant J; Pierce, Casey M; Wijdicks, Coen A; Laprade, Robert F

2014-03-01

Complete radial tears near the medial meniscus posterior root attachment site disrupt the circumferential integrity of the meniscus (similar to a posterior root avulsion). These tears can compromise the circumferential integrity, and they have been reported in biomechanical studies to be comparable with the meniscectomized state. To quantify the tibiofemoral contact pressure and contact area changes that occur in cadaveric knees from complete posterior horn radial tears and subsequent repairs of the medial meniscus adjacent to the posterior root attachment site. Controlled laboratory study. Six nonpaired fresh-frozen human cadaveric knees each underwent 45 different testing conditions: 9 medial meniscus conditions (intact, root avulsion, root repair, serial radial tear at 3, 6, and 9 mm from the root attachment site, and in situ repair at the same 3 distances from the root attachment site) at 5 flexion angles (0°, 30°, 45°, 60°, and 90°), under a 1000-N axial load. Tekscan sensors were used to measure contact area and pressure in the medial and lateral compartments. The medial meniscus root avulsion and all radial tear conditions resulted in significantly decreased contact area and increased mean contact pressure compared with the intact state for knee flexion angles beyond 0° (P < .05). The root repair and in situ repairs restored contact area and pressure to levels statistically indistinguishable from those of the intact meniscus and increased contact area and decreased contact pressure compared with the corresponding tear conditions. Posterior horn radial tears adjacent to the medial meniscus root that extend to the meniscocapsular junction can lead to derangement of the loading profiles of the medial compartment that are similar to a root avulsion. Repair of these radial tears with an in situ pull-out technique restored joint mechanics to the intact state. Complete radial tears of the posterior horn of the medial meniscus, which occur relatively frequently, are biomechanically equivalent to root avulsions and could potentially lead to medial compartment arthrosis. An in situ repair offers an alternative treatment to meniscectomy and can reestablish the posterior anchor point, thus improving load distribution in the medial compartment. Future clinical studies of these repairs are recommended.

RootScan: Software for high-throughput analysis of root anatomical traits

USDA-ARS?s Scientific Manuscript database

RootScan is a program for semi-automated image analysis of anatomical phenes in root cross-sections. RootScan uses pixel value thresholds to separate the cross-section from its background and to visually dissect it into tissue regions. Area measurements and object counts are performed within various...

Pasture degradation modifies soil organic matter properties and biochemical functioning in Tibetan grasslands

NASA Astrophysics Data System (ADS)

Spielvogel, Sandra; Steingräber, Laura; Schleuß, Per; Kuzyakov, Yakov; Guggenberger, Georg

2015-04-01

Kobresia pastures of the Tibetan Plateau represent the world's largest alpine ecosystem. Moderate husbandry on Kobresia pastures is beneficial for the storage of soil organic carbon (OC), nitrogen (N) and other nutrients and prevents erosion by establishment of sedge-turf root mats with high OC allocation rates below ground. However, undisturbed root mats are affected by freezing and thawing processes, which cause initial ice cracks. As a consequence decomposition of root mat layers will be accelerated and current sedentarization programs with concomitant increased grazing intensity may additionally enhance root mat degradation. Finally, cracks are enlarged by water and wind erosion as well as pika activities until bare soil surface areas without root mat horizons occur. The aim of this study was to understand the impact of the root mat layer on soil organic carbon stabilization and microbial functioning depending on soil depths and to predict future changes (OC, N and nutrient losses, soil microbial functioning in SOM transformation) by overgrazing and climate change. We investigated the mineral soil below Kobresia root mats along a false time degradation sequence ranging from stage 1 (intact root mat) to stage 4 (mats with large cracks and bare soil patches). Vertical gradients of δ13C values, neutral sugar, cutin and suberin contents as well as microbial biomass estimated by total phospholipid fatty acid (PLFA), microbial community composition (PLFA profiles) and activities of six extracellular enzymes involved in the C, N, and P cycle were assessed. Soil OC and N contents as well as C/N ratios indicate an increasing illuviation of topsoil material into the subsoil with advancing root mat degradation. This was confirmed by more negative δ13C values as well as significantly (p ≤ 0.05) increasing contributions of cutin derived hydroxy fatty acids to OC in the subsoils from degradation stages 1 to 4. PLFA profiles were surprisingly similar in the subsoils of degradation stages 1, 2 and 3 although OC contents and composition in the subsoil changed progressively from stage 1 to 4. Only the PLFA profiles of stage 4 differed from those of the other subsoils, suggesting that microbial communities were mainly controlled by other factors than C and N contents and SOM composition. These findings were also confirmed by the activities of β-glucosidase, xylanase, amino-peptidases and proteases. Those enzyme activities were highest in the subsoil of degradation stage 4, whereas degradation stages 2 and 3 showed low enzyme activities in the subsoil if related to soil OC amount and composition. We conclude that pasture degradation decreases not only mechanical protection of soil surface by Kobresia root mats, but also changes their biochemical and microbial functions.

CBVT analysis of canal configuration of the mesio-buccal root of maxillary first permanent molar teeth: An in vitro study.

PubMed

Shenoi, R Pratima; Ghule, Hrishikesh M

2012-07-01

For successful endodontic treatment, it's imperative to locate and obturate all root canals. As concluded by Ingle, the major cause for failure of root canal therapy is in ability to recognize all theexisting canals and subsequent failure in their obturation. To analyze the canal configuration of the mesio-buccal root of maxillary first permanent molar teeth. A total of 30 recently extracted human permanent maxillary first molars were collected and stored in a container with 5% Sodium Chloride solution for four days. Teeth with open apices, external resorption, improperly formed roots and teeth with previous restorations were excluded. Using the dental modeling wax, teeth were arranged in a 'U' shaped arch with roots embedded inside the wax and occlusal surface remaining free. 10 teeth were arranged in each arch and three such sample plates were prepared. Flat surface of the base encasing enabled the plate to be mounted on flat plastic bite plate. With bite plate roughly centered in the focal trough area Axial, Coronal and Sagittal section Cone-Beam Computed Tomography (CBCT) images were taken with Kodak 9000 Extra-oral Imaging System. Images displayed on a monitor were inspected by two endodontists using Kodak Dental Imaging Software 3D Module V2.2. When the data was observed, it was found that 24 teeth out of total 30 teeth examined showed some variation (i.e. possible additional canal) along the length of the mesio-buccal root canal. Out of these 24 teeth, 13 showed presence of additional canal at coronal third, 7 showed presence of additional canal at middle third and four showed presence of additional canal in apical third level. Percentage analysis was done as there was no group comparison to be done. Cone-Beam Volumetric Tomography (CBVT) evaluation positively identified the variations in mesio-buccal canal in 80% of samples. Out of these, 54.16% were in coronal 3(rd), 29.16% in middle 3(rd) and 16.66% were in apical 3(rd). Within the limitations of this study, it can be concluded that- (1) more than half of maxillary first molars have four canals and (2) most of the additional canals were located in the MBR and CBVT is a good diagnostic tool to help diagnose these additional canals. Further investigations using larger sample sizes would be helpful.

Viscoelastic Vibration Dampers for Turbomachine Blades

NASA Technical Reports Server (NTRS)

Nguyen, Nhan

2003-01-01

Simple viscoelastic dampers have been invented for use on the root attachments of turbomachine blades. These dampers suppress bending- and torsion-mode blade vibrations, which are excited by unsteady aerodynamic forces during operation. In suppressing vibrations, these dampers reduce fatigue (thereby prolonging blade lifetimes) while reducing noise. These dampers can be installed in new turbomachines or in previously constructed turbomachines, without need for structural modifications. Moreover, because these dampers are not exposed to flows, they do not affect the aerodynamic performances of turbomachines. Figure 1 depicts a basic turbomachine rotor, which includes multiple blades affixed to a hub by means of dovetail root attachments. Prior to mounting of the blades, thin layers of a viscoelastic material are applied to selected areas of the blade roots. Once the blades have been installed in the hub and the rotor is set into rotation, centrifugal force compresses these layers between the mating load-bearing surfaces of the hub and the blade root. The layers of viscoelastic material provide load paths through which the vibration energy of the blade can be dissipated. The viscoelasticity of the material converts mechanical vibration energy into shear strain energy and then from shear strain energy to heat. Of the viscoelastic materials that have been considered thus far for this application, the one of choice is a commercial polyurethane that is available in tape form, coated on one side with an adhesive that facilitates bonding to blade roots. The thickness of the tape can be chosen to suit the specific application. The typical thickness of 0.012 in. (.0.3 mm) is small enough that the tape can fit in the clearance between the mating blade-root and hub surfaces in a typical turbomachine. In an experiment, a blade was mounted in a test fixture designed to simulate the blade-end conditions that prevail in a turbocompressor. Vibrations were excited in the blade by use of an impact hammer, and damping of the vibrations was measured by use of a dynamic signal analyzer. Tests were performed without and with viscoelastic dampers installed in the dovetail root attachment. The results of the measurements, some of which are presented in Figure 2, show that the viscoelastic dampers greatly increased the rate of damping of vibrations. Accordingly, dynamic stresses on rotor blades were significantly reduced, as shown in Figure 2.

Hounsfield unit change in root and alveolar bone during canine retraction.

PubMed

Jiang, Feifei; Liu, Sean S-Y; Xia, Zeyang; Li, Shuning; Chen, Jie; Kula, Katherine S; Eckert, George

2015-04-01

The objective of this study was to determine the Hounsfield unit (HU) changes in the alveolar bone and root surfaces during controlled canine retractions. Eighteen maxillary canine retraction patients were selected for this split-mouth design clinical trial. The canines in each patient were randomly assigned to receive either translation or controlled tipping treatment. Pretreatment and posttreatment cone-beam computed tomography scans of each patient were used to determine tooth movement direction and HU changes. The alveolar bone and root surface were divided into 108 divisions, respectively. The HUs in each division were measured. Mixed-model analysis of variance was applied to test the HU change distribution at the P <0.05 significance level. The HU changes varied with the directions relative to the canine movement. The HU reductions occurred at the root surfaces. Larger reductions occurred in the divisions that were perpendicular to the moving direction. However, HUs decreased in the alveolar bone in the moving direction. The highest HU reduction was at the coronal level. HU reduction occurs on the root surface in the direction perpendicular to tooth movement and in the alveolar bone in the direction of tooth movement when a canine is retracted. Copyright © 2015 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.

Hounsfield Unit Change in Root and Alveolar Bone during Canine Retraction

PubMed Central

Jiang, Feifei; Liu, Sean Y.; Xia, Zeyang; Li, Shuning; Chen, Jie; Kula, Katherine S.; Eckert, George

2014-01-01

Objectives The objective of this study was to determine the Hounsfield unit (HU) changes in the alveolar bone and root surface during controlled canine retractions. Methods Eighteen maxillary canine retraction patients were selected for this split mouth design clinical trial. The canines in each patient were randomly assigned to receive either translation or controlled tipping treatment strategy. Pre- and post-treatment cone beam computed tomography scans of each patient were used to determine tooth movement direction and HU changes. The alveolar bone and root surface were divided into 108 divisions, respectively. The HU in each division was measured. The Mixed-model ANOVA was applied to test the HU change distribution at the p<0.05 significant level. Results The HU changes varied with the directions relative to the canine movement. The HU reduction occurred at the root surface. Larger reductions occurred in the divisions that were perpendicular to the moving direction. However, HU decreased in the alveolar bone in the moving direction. The highest HU reduction was at the coronal level. Conclusions HU reduction occurs on the root surface in the direction perpendicular to the tooth movement and in the alveolar bone in the direction of tooth movement when a canine is retracted. PMID:25836004

[Cell surface peroxidase--generator of superoxide anion in wheat root cells under wound stress].

PubMed

Chasov, A V; Gordon, L Kh; Kolesnikov, O P; Minibaeva, F V

2002-01-01

Development of wound stress in excised wheat roots is known to be accompanied with an increase in reactive oxygen species (ROS) production, fall of membrane potential, release of K+ from cells, alkalization of extracellular solution, changes in respiration and metabolism of structural lipids. Dynamics of superoxide release correlates with changes in other physiological parameters, indicating the cross-reaction of these processes. Activity of peroxidase in extracellular solution after a 1 h incubation and removal of roots was shown to be stimulated by the range of organic acids, detergents, metals, and to be inhibited by cyanide. Superoxide production was sensitive to the addition of Mn2+ and H2O2. Increase in superoxide production correlates with the enhancement of peroxidase activity at the application of organic acids and detergents. The results obtained indicate that cell surface peroxidase is one of the main generators of superoxide in wounded wheat root cells. Different ways of stimulation of the ROS producing activity in root cells is supposed. By controlling superoxide and hydrogen peroxide formation, the cell surface peroxidase can control the adaptation processes in stressed plant cells.

Effect of endodontic irrigating solutions on the microhardness and roughness of root canal dentin: an in vitro study.

PubMed

Patil, Chetan R; Uppin, Veerendra

2011-01-01

To evaluate the effect of widely used endodontic irrigating solutions on root dentin microhardness and surface roughness. One hundred twenty, non-carious extracted human permanent incisor teeth were selected. The crowns of the teeth were sectioned and the roots were separated longitudinally to get 240 specimens. These specimens were then divided into six groups according to the irrigating solutions used. The solutions used were 5% and 2.5% NaOCl solutions, 3% H2 O2 , 17% EDTA solution, 0.2% chlorhexidine gluconate, and distilled water. Then, the specimens were subjected to microhardness and roughness testing. The data were analyzed using ANOVA and Tukey's multiple comparison tests. The results of this study indicated that all irrigation solutions, except 0.2% chlorhexidine gluconate, decreased the microhardness of root dentin, and 3% H2 O2 and 0.2% chlorhexidine gluconate had no effect on surface roughness. Within the limitation of this study, it is concluded that 0.2% chlorhexidine gluconate seems to be an appropriate irrigation solution, because of its harmless effect on the microhardness and surface roughness of root canal dentin.

Chemical Interactions of Uranium in Water, Sediments, and Plants Along a Watershed Adjacent to the Abandoned Jackpile Mine

NASA Astrophysics Data System (ADS)

Blake, J.; De Vore, C. L.; Avasarala, S.; Ali, A.; Roldan, C.; Bowers, F.; Spilde, M.; Artyushkova, K.; Cerrato, J.

2015-12-01

The chemical interactions, mobility, and plant uptake of uranium (U) near abandoned mine wastes was investigated along the Rio Paguate, adjacent to the Jackpile Mine, located in Laguna Pueblo, New Mexico. Elevated U concentrations in surface water adjacent to mine waste range from 30 to 710 μg/L seasonally and decrease to 5.77 to 10.0 μg/L at a wetland 4.5 kilometers downstream of the mine. Although U concentrations in stream water are elevated, aqua regia acid digestions performed on co-located stream bed and stream bank sediments reveal that there is limited U accumulation on sediments along the reach between the mine and wetland, with most sediment concentrations being near the 3 mg/kg crustal average. However, U concentrations in sediments in the wetland are 4 times the background concentrations in the area. Individual results from salt cedar roots, stems, and leaves collected along the river transect show higher U concentrations in the roots adjacent to the mine waste (20 and 55 mg/kg) and lower in the stems and leaves. Translocation values calculated below 1 are evident in many of the plant samples, suggesting that U root to shoot translocation is minimal and U is accumulating in the roots. Concentrations of U in salt cedar roots from downstream of the mine waste decrease to 15 mg/kg. X-ray photoelectron spectroscopy analysis on sediment samples adjacent to the mine waste show a 75:25% ratio of Fe(III) to Fe(II), which can have an effect on adsorption properties. Electron microprobe results suggest that the ore in this area is present as a uranium-phosphate phase. Our results suggest that dilution, uptake by plants, and U sorption to wetland sediments are the dominant factors that help to decrease the U concentrations downstream of the mine.

Regional structural characteristics of bovine periodontal ligament samples and their suitability for biomechanical tests.

PubMed

Bosshardt, Dieter D; Bergomi, Marzio; Vaglio, Giovanna; Wiskott, Anselm

2008-03-01

Mechanical testing of the periodontal ligament requires a practical experimental model. Bovine teeth are advantageous in terms of size and availability, but information is lacking as to the anatomy and histology of their periodontium. The aim of this study, therefore, was to characterize the anatomy and histology of the attachment apparatus in fully erupted bovine mandibular first molars. A total of 13 teeth were processed for the production of undecalcified ground sections and decalcified semi-thin sections, for NaOH maceration, and for polarized light microscopy. Histomorphometric measurements relevant to the mechanical behavior of the periodontal ligament included width, number, size and area fraction of blood vessels and fractal analysis of the two hard-soft tissue interfaces. The histological and histomorphometric analyses were performed at four different root depths and at six circumferential locations around the distal and mesial roots. The variety of techniques applied provided a comprehensive view of the tissue architecture of the bovine periodontal ligament. Marked regional variations were observed in width, surface geometry of the two bordering hard tissues (cementum and alveolar bone), structural organization of the principal periodontal ligament connective tissue fibers, size, number and numerical density of blood vessels in the periodontal ligament. No predictable pattern was observed, except for a statistically significant increase in the area fraction of blood vessels from apical to coronal. The periodontal ligament width was up to three times wider in bovine teeth than in human teeth. The fractal analyses were in agreement with the histological observations showing frequent signs of remodeling activity in the alveolar bone - a finding which may be related to the magnitude and direction of occlusal forces in ruminants. Although samples from the apical root portion are not suitable for biomechanical testing, all other levels in the buccal and lingual aspects of the mesial and distal roots may be considered. The bucco-mesial aspect of the distal root appears to be the most suitable location.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fred J. Molz, III

To better understand longer-term vadose zone transport in southeastern soils, field lysimeter experiments were conducted at the Savannah River Site (SRS) near Aiken, SC, in the 1980s. Each of the three lysimeters analyzed herein contained a filter paper spiked with different Pu solutions, and they were left exposed to natural environmental conditions (including the growth of annual weed grasses) for 11 years. The resulting Pu activity measurements from each lysimeter core showed anomalous activity distributions below the source, with significant migration of Pu above the source. Such results are not explainable by adsorption phenomena alone. A transient variably saturated flowmore » model with root water uptake was developed and coupled to a soil reactive transport model. Somewhat surprisingly, the fully transient analysis showed results nearly identical to those of a much simpler steady flow analysis performed previously. However, all phenomena studied were unable to produce the upward Pu transport observed in the data. This result suggests another transport mechanism such as Pu uptake by roots and upward transport due to transpiration. Thus, the variably saturated flow and reactive transport model was extended to include uptake and transport of Pu within the root xylem, along with computational methodology and results. In the extended model, flow velocity in the soil was driven by precipitation input along with transpiration and drainage. Water uptake by the roots determined the flow velocity in the root xylem, and this along with uptake of Pu in the transpiration stream drove advection and dispersion of the two Pu species in the xylem. During wet periods with high potential evapotranspiration, maximum flow velocities through the xylem would approached 600 cm/hr, orders of magnitude larger that flow velocities in the soil. Values for parameters and the correct conceptual viewpoint for Pu transport in plant xylem was uncertain. This motivated further experiments devoted to Pu uptake by corn roots and xylem transport. Plants were started in wet paper wrapped around each corn seed. When the tap roots were sufficiently long, the seedlings were transplanted to a soil container with the tap root extending out the container bottom. The soil container was then placed over a nutrient solution container, and the solution served as an additional medium for root growth. To conduct an uptake study, a radioactive substance, such as Pu complexed with the bacterial siderophore DFOB, was added to the nutrient solution. After a suitable elapsed time, the corn plant was sacrificed, cut into 10 cm lengths, and the activity distribution measured. Experimental results clarified the basic nature of Pu uptake and transport in corn plants, and resulting simulations suggested that each growing season Pu in the SRS lysimeters would move into the plant shoots and be deposited on the soil surface during the Fall dieback. Subsequent isotope ratio analyses showed that this did happen. OVERALL RESULTS AND CONCLUSIONS - (1) Pu transport downward from the source is controlled by advection, dispersion and adsorption, along with surface-mediated REDOX reactions. (2) Hysteresis, extreme root distribution functions, air-content dependent oxidation rate constants, and large evaporation rates from the soil surface were not able to explain the observed upward migration of Pu. (3) Small amounts of Pu uptake by plant roots and translocation in the transpiration stream creates a realistic mechanism for upward Pu migration (4) Realistic xylem cross-sectional areas imply high flow velocities under hot, wet conditions. Such flow velocities produce the correct shape for the observed activity distributions in the top 20 cm of the lysimeter soil. (5) Simulations imply that Pu should have moved into the above-ground grass tissue each year during the duration of the experiments, resulting in an activity residual accumulating on the soil surface. An isotope ratio analysis showed that the observed surface Pu residue was from the buried sources, not atmospheric fallout. (6) The plant experiments indicate a Pu-DFOB velocity in the corn xylem of at least 174 cm/hr, much higher than ionic Pu in soil. Thus, Pu complexation with chelating agents is probably what led to the observed enhanced uptake and mobility in grasses. (7) Plant experiments show that the uptake of Fe-DFOB, Pu-DFOB and the resulting distributions are very similar. This supports the hypothesis that plant and bacterial iron-seeking chemistry mistakes Pu for Fe.« less

Evaluation of the Effects of Er,Cr:YSGG Laser, Ultrasonic Scaler and Curette on Root Surface Profile Using Surface Analyser and Scanning Electron Microscope: An In Vitro Study.

PubMed

Arora, Shipra; Lamba, Arundeep Kaur; Faraz, Farrukh; Tandon, Shruti; Ahad, Abdul

2016-01-01

Introduction: The periodontal therapy is primarily targeted at removal of dental plaque and plaque retentive factors. Although the thorough removal of adherent plaque, calculus and infected root cementum is desirable, it is not always achieved by conventional modalities. To accomplish more efficient results several alternative devices have been used. Lasers are one of the most promising modalities for nonsurgical periodontal treatment as they can achieve excellent tissue ablation with strong bactericidal and detoxification effects. Methods: Thirty freshly extracted premolars were selected and decoronated. The mesial surface of each root was divided vertically into four approximately equal parts. These were distributed into four group based on the root surface treatment. Part A (n = 30) was taken as control and no instrumentation was performed. Part B (n = 30) was irradiated by Erbium, Chromium doped Yttrium Scandium Gallium Garnet (Er,Cr:YSGG) laser. Part C (n = 30) was treated by piezoelectric ultrasonic scaler. Part D (n = 30) was treated by Gracey curette. The surface roughness was quantitatively analyzed by profilometer using roughness average (Ra) value, while presence of smear layer, cracks, craters and melting of surface were analyzed using scanning electron microscope (SEM). The means across the groups were statistically compared with control using Dunnett test. Results: Among the test groups, Er,Cr:YSGG laser group showed maximum surface roughness (mean Ra value of 4.14 μm) as compared to ultrasonic scaler (1.727 μm) and curette group (1.22 μm). However, surface with smear layer were found to be maximum (50%) in curette treated samples and minimum (20%) in laser treated ones. Maximum cracks (83.34%) were produced by ultrasonic scaler, and minimum (43.33%) by curettes. Crater formation was maximum (50%) in laser treated samples and minimum (3.33%) in curette treated ones. 63.33% samples treated by laser demonstrated melting of root surface, followed by ultrasonic scaler and curettes. Conclusion: Er,Cr:YSGG laser produced maximum microstructural changes on root surface that can influence the attachment of soft periodontal tissues as well as plaque and calculus deposition. In vivo studies are needed to validate these results and to evaluate their clinical effects.

Validation of HF Radar ocean surface currents in the Ibiza Channel using lagrangian drifters, moored current meter and underwater gliders

NASA Astrophysics Data System (ADS)

Lana, Arancha; Fernández, Vicente; Orfila, Alejandro; Troupin, Charles; Tintoré, Joaquín

2015-04-01

SOCIB High Frequency (HF) radar is one component of a multi-platform system located in the Balearic Islands and made up of Lagrangian platforms (profilers and drifting buoys), fixed stations (sea-level, weather, mooring and coastal), beach monitoring (camera), gliders, a research vessel as well as an ocean forecast system (waves and hydrodynamics). The HF radar system overlooks the Ibiza Channel, known as a 'choke point" where Atlantic and Mediterranean water masses interact and where meridional exchanges of water mass properties between the Balearic and the Algerian sub-basins take place. In order to determine the reliability of surface velocity measurements in this area, a quality assessment of the HF Radar is essential. We present the results of several validation experiments performed in the Ibiza Channel in 2013 and 2014. Of particular interest is an experiment started in September 2014 when a set of 13 surface drifters with different shapes and drogue lengths were released in the area covered by the HF radar. The drifter trajectories can be examined following the SOCIB Deployment Application (DAPP): http://apps.socib.es/dapp. Additionally, a 1-year long time series of surface currents obtained from a moored surface current-meter located in the Ibiza Channel, inside the area covered by the HF radar, was also used as a useful complementary validation exercise. Direct comparison between both radial surface currents from each radar station and total derived velocities against drifters and moored current meter velocities provides an assessment of the HF radar data quality at different temporal periods and geographical areas. Statistics from these comparisons give good correlation and low root-mean-square deviation. The results will be discussed for different months, geographical areas and types of surface drifters and wind exposure. Moreover, autonomous underwater glider constitutes an additional source of information for the validation of the observed velocity structures and some statistics will be presented.

Does altered aortic flow in marfan syndrome relate to aortic root dilatation?

PubMed

Wang, Hung-Hsuan; Chiu, Hsin-Hui; Tseng, Wen-Yih Isaac; Peng, Hsu-Hsia

2016-08-01

To examine possible hemodynamic alterations in adolescent to adult Marfan syndrome (MFS) patients with aortic root dilatation. Four-dimensional flow MRI was performed in 20 MFS patients and 12 age-matched normal subjects with a 3T system. The cross-sectional areas of 10 planes along the aorta were segmented for calculating the axial and circumferential wall shear stress (WSSaxial , WSScirc ), oscillatory shear index (OSIaxial , OSIcirc ), and the nonroundness (NR), presenting the asymmetry of segmental WSS. Pearson's correlation analysis was performed to present the correlations between the quantified indices and the body surface area (BSA), aortic root diameter (ARD), and Z score of the ARD. P < 0.05 indicated statistical significance. Patients exhibited lower WSSaxial in the aortic root and the WSScirc in the arch (P < 0.05-0.001). MFS patients exhibited higher OSIaxial and OSIcirc in the sinotubular junction and arch, but lower OSIcirc in the descending aorta (all P < 0.05). The NR values were lower in patients (P < 0.05). The WSSaxial or WSScirc exhibited moderate to strong correlations with BSA, ARD, or Z score (R(2)  = 0.50-0.72) in MFS patients. The significant differences in the quantified indices, which were associated with BSA, ARD, or Z score, in MFS were opposite to previous reports for younger MFS patients, indicating that altered flows in MFS patients may depend on the disease progress. The possible time dependency of hemodynamic alterations in MFS patients strongly suggests that longitudinal follow-up of 4D Flow is needed to comprehend disease progress. J. Magn. Reson. Imaging 2016;44:500-508. © 2016 Wiley Periodicals, Inc.

Does altered aortic flow in marfan syndrome relate to aortic root dilatation?

PubMed Central

Wang, Hung‐Hsuan; Chiu, Hsin‐Hui; Tseng, Wen‐Yih Isaac

2016-01-01

Purpose To examine possible hemodynamic alterations in adolescent to adult Marfan syndrome (MFS) patients with aortic root dilatation. Materials and Methods Four‐dimensional flow MRI was performed in 20 MFS patients and 12 age‐matched normal subjects with a 3T system. The cross‐sectional areas of 10 planes along the aorta were segmented for calculating the axial and circumferential wall shear stress (WSSaxial, WSScirc), oscillatory shear index (OSIaxial, OSIcirc), and the nonroundness (NR), presenting the asymmetry of segmental WSS. Pearson's correlation analysis was performed to present the correlations between the quantified indices and the body surface area (BSA), aortic root diameter (ARD), and Z score of the ARD. P < 0.05 indicated statistical significance. Results Patients exhibited lower WSSaxial in the aortic root and the WSScirc in the arch (P < 0.05–0.001). MFS patients exhibited higher OSIaxial and OSIcirc in the sinotubular junction and arch, but lower OSIcirc in the descending aorta (all P < 0.05). The NR values were lower in patients (P < 0.05). The WSSaxial or WSScirc exhibited moderate to strong correlations with BSA, ARD, or Z score (R2 = 0.50–0.72) in MFS patients. Conclusion The significant differences in the quantified indices, which were associated with BSA, ARD, or Z score, in MFS were opposite to previous reports for younger MFS patients, indicating that altered flows in MFS patients may depend on the disease progress. The possible time dependency of hemodynamic alterations in MFS patients strongly suggests that longitudinal follow‐up of 4D Flow is needed to comprehend disease progress. J. Magn. Reson. Imaging 2016;44:500–508. PMID:26854646

Effect of indomethacin on surface treatment and intracanal dressing of replanted teeth in dogs.

PubMed

Zanetta-Barbosa, Darceny; Moura, Camilla Christian Gomes; Machado, Juliana Reis; Crema, Virginia Oliveira; Lima, Cirilo Antônio de Paula; de Carvalho, Antônio Cesar Perry

2014-01-01

This study evaluated the healing process of teeth replanted after root treatment and intracanal dressing with indomethacin alone or indomethacin with calcium hydroxide (Ca[OH]2). Through a case-control study, 24 teeth of 6 adult dogs were extracted, dried, and divided into 4 groups according to the root surface treatment protocols performed before replantation and the intracanal medication used after replantation. In group 1 (negative control), root surfaces were treated by immersion in a 0.9% saline solution and then replanted. In the other groups, the roots were immersed for 10 minutes in Ca(OH)2 (group 2), indomethacin (group 3), or a solution of indomethacin and Ca(OH)2 (group 4). After 2 weeks, group 1 teeth were subjected to single-visit root canal treatment and obturation with gutta-percha and sealer consisting of zinc oxide and eugenol. The teeth in the other groups were subjected to intracanal dressing with the same material used for immersion. After an additional period of 28 weeks, the animals were euthanized and the jaws containing the replanted teeth were processed for histologic analysis. Histometric values were statistically analyzed, with significance set at a P value less than or equal to .05. Group 1 exhibited significantly more normal periodontium than group 4 (P = .02). Total resorption was greater in group 4 than in group 1 (P = .02). No statistically significant difference in the percentage of surface resorption or in total inactive resorption was observed between the groups. The findings of this study suggest that intracanal dressing and topical root treatment with Ca(OH)2 with or without indomethacin is not recommended for teeth dried for 50 minutes, but the use of indomethacin alone as root surface treatment for delayed tooth replantation deserves further study using longer drying periods. In addition, the present results suggest that a single-visit root canal, performed up to 2 weeks after replantation, might be indicated for teeth dried for up to 50 minutes. Copyright © 2014 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

Effects of EDTA gel preconditioning of periodontally affected human root surfaces on chlorhexidine substantivity - an SEM study.

PubMed

Gamal, Ahmed Y; Mailhot, Jason M

2007-09-01

Infection control is an important requirement during the early stages of periodontal healing. This study was performed to assess the preconditioning effect of EDTA gel on chlorhexidine (CHX) substantivity to periodontally involved root surfaces. Eighty patients with severe chronic periodontitis were enrolled in this study. Following cause-related therapy, patients were divided randomly into four groups. Each group consisted of 20 subjects with one tooth that was diagnosed as hopeless and designated for extraction. In group 1 (G1), selected periodontal pockets were filled with a placebo gel in a silica base for 2 minutes. Exposed roots in group 2 (G2) were etched for 2 minutes with a neutral EDTA conditioning agent, followed by pocket fill with the placebo gel. Pockets in group 3 (G3) were filled with 0.12% CHX digluconate gel in a silica base. Exposed roots in group 4 (G4) were etched for 2 minutes with a neutral EDTA conditioning agent, followed by pocket fill with the CHX gel. Four teeth from each group were extracted immediately and at 3, 12, 24, and 48 hours for SEM evaluation. G1 and G2 specimens showed no evidence of silica adherent to any of the examined root surfaces. At 3 hours following CHX gel application, G3 specimens showed marked reduction in CHX-coated silica. At 24 and 48 hours following EDTA and CHX gel application, G4 specimens demonstrated adherent CHX-coated silica particles despite the reclogging of the tubule orifices. EDTA and CHX gel root conditioning is a valuable regimen that improves CHX substantivity to periodontally involved root surfaces.

Challenges to estimating whole forest root biomass with ground penetrating radar

NASA Astrophysics Data System (ADS)

Butnor, J. R.

2016-12-01

Over the past two decades, substantial technical advances have been made in detecting tree roots with ground penetrating radar (GPR). Under favorable soil dielectric conditions, root location, depth, diameter and mass estimates are possible in the field. With careful notation of survey lines, three dimensional reconstructions of root architecture may also be achieved. The technique has been very useful for quantifying lateral root biomass in silvicultural studies, but is not yet a standalone technique for estimating root biomass in forests. The purpose of this presentation is to highlight the limitations of GPR in the field to stimulate discussion on how to overcome these challenges. Under field conditions, surface-based antennas with frequencies of 400 to 1500 MHz cannot detect fine roots (<2 mm diameter), vertical taproots, below-stump mass, decayed roots or separate roots by species. Higher frequency antennas designed for concrete inspection are available, but penetration through forest soils would be marginal. Over half of the root mass in many Pinus species is in the taproot which is undetectable in part or whole by GPR. This presents challenges to stand-level quantification as whole classes of biomass and structures are not reliably detected. Lack of automation of data processing and interpretation steps currently makes data analysis arduous and in some cases subject to interpretation by an expert user. Forests have a high degree of heterogeneity in surface conditions (e.g., holes, soil moisture, stems, woody and herbaceous plants) that may prevent antennas from coupling with the surface to propagate EM waves and receive reflections. What is the potential for open source data analysis programs to be developed and shared? How will new digital, multi-frequency antennas improve resolution? Can air launched antennas be developed that have both the depth penetration and resolution to detect roots? Are purpose-designed bore hole antenna needed for imaging taproots?

Possible Benefits of Mycorrhizal Symbiosis, in Reducing CO2 from Environment

NASA Astrophysics Data System (ADS)

Azmat, Rafia

2013-12-01

It is a fact that the relationship between a fungus and a plant can have a great impact on the environment, especially under drought conditions. Experiments conducted at the laboratory scale suggested that in mycorrhizal symbiosis; plants usually provide their fungal partners with carbohydrates from photosynthesis and receive mineral nutrients. It is observed that mycorrhizal inoculated plants observed large surface area of leaves and outsized root sections which were helpful in increasing the rate of photosynthetic processes. This may be attributed to the rapid production of carbohydrate for their fungal mate. The same phenomena can be observed in environments of high traffic density or waste burning, industrial zones (where there are emissions of CO2 from chimneys) or the areas that are lack nutrients such as nitrogen and phosphorus. It may be observed that the plants that have this association with mycorrhizal fungi may obligate a better chance in inhabiting this area. These plants can be helpful in reducing the CO2 from the polluted atmosphere. The large length of the roots were related to the absorption of water molecules for survival as well as formation of first organic complex CHO for providing the energy to the plant in biotic stress and C and nutrient exchange between fungal partner and plants.

A Comparative Study of Shaping Ability of four Rotary Systems.

PubMed

Rubio, Jorge; Zarzosa, José Ignacio; Pallarés, Antonio

2015-12-01

This study compared the cutting area, instrumentation time, root canal anatomy preservation and non-instrumented areas obtained by F360(®), Mtwo(®), RaCe(®) and Hyflex(®) files with ISO size 35. 120 teeth with a single straight root and root canal were divided into 4 groups. Working length was calculated by using X-rays. The teeth were sectioned with a handpiece and a diamond disc, and the sections were observed with Nikon SMZ-2T stereoscopic microscope and an Intralux 4000-1 light source. The groups were adjusted with a preoperative analysis with AutoCAD. The teeth were reconstructed by a #10 K-File and epoxy glue. Each group was instrumented with one of the four file systems. The instrumentation time was calculated with a 1/100 second chronometer. The area of the thirds and root canal anatomy preservation were analyzed with AutoCAD 2013 and the non-instrumented areas with AutoCAD 2013 and SMZ-2T stereoscopic microscope. The statistical analysis was made with Levene's Test, ANOVA, Bonferroni Test and Pearson´s Chi-square. Equal variances were shown by Levene's Test (P > 0.05). ANOVA (P > 0.05) showed the absence of significant differences. There were significant differences in the instrumentation time (P < 0.05). For root canal anatomy preservation and non-instrumented areas, there were no significant differences between all systems (P > 0.05). The 4 different rotary systems produced similar cutting area, root canal anatomy preservation and non-instrumented areas. Regarding instrumentation time, F360(®) was the fastest system statistically.

A Comparative Study of Shaping Ability of four Rotary Systems

PubMed Central

Zarzosa, José Ignacio; Pallarés, Antonio

2015-01-01

Purpose This study compared the cutting area, instrumentation time, root canal anatomy preservation and non-instrumented areas obtained by F360®, Mtwo®, RaCe® and Hyflex® files with ISO size 35. Material and Methods 120 teeth with a single straight root and root canal were divided into 4 groups. Working length was calculated by using X-rays. The teeth were sectioned with a handpiece and a diamond disc, and the sections were observed with Nikon SMZ-2T stereoscopic microscope and an Intralux 4000-1 light source. The groups were adjusted with a preoperative analysis with AutoCAD. The teeth were reconstructed by a #10 K-File and epoxy glue. Each group was instrumented with one of the four file systems. The instrumentation time was calculated with a 1/100 second chronometer. The area of the thirds and root canal anatomy preservation were analyzed with AutoCAD 2013 and the non-instrumented areas with AutoCAD 2013 and SMZ-2T stereoscopic microscope. The statistical analysis was made with Levene’s Test, ANOVA, Bonferroni Test and Pearson´s Chi-square. Results Equal variances were shown by Levene’s Test (P > 0.05). ANOVA (P > 0.05) showed the absence of significant differences. There were significant differences in the instrumentation time (P < 0.05). For root canal anatomy preservation and non-instrumented areas, there were no significant differences between all systems (P > 0.05). Conclusions The 4 different rotary systems produced similar cutting area, root canal anatomy preservation and non-instrumented areas. Regarding instrumentation time, F360® was the fastest system statistically. PMID:27688412

A high-performance ground-based prototype of horn-type sequential vegetable production facility for life support system in space

NASA Astrophysics Data System (ADS)

Fu, Yuming; Liu, Hui; Shao, Lingzhi; Wang, Minjuan; Berkovich, Yu A.; Erokhin, A. N.; Liu, Hong

2013-07-01

Vegetable cultivation plays a crucial role in dietary supplements and psychosocial benefits of the crew during manned space flight. Here we developed a ground-based prototype of horn-type sequential vegetable production facility, named Horn-type Producer (HTP), which was capable of simulating the microgravity effect and the continuous cultivation of leaf-vegetables on root modules. The growth chamber of the facility had a volume of 0.12 m3, characterized by a three-stage space expansion with plant growth. The planting surface of 0.154 m2 was comprised of six ring-shaped root modules with a fibrous ion-exchange resin substrate. Root modules were fastened to a central porous tube supplying water, and moved forward with plant growth. The total illuminated crop area of 0.567 m2 was provided by a combination of red and white light emitting diodes on the internal surfaces. In tests with a 24-h photoperiod, the productivity of the HTP at 0.3 kW for lettuce achieved 254.3 g eatable biomass per week. Long-term operation of the HTP did not alter vegetable nutrition composition to any great extent. Furthermore, the efficiency of the HTP, based on the Q-criterion, was 7 × 10-4 g2 m-3 J-1. These results show that the HTP exhibited high productivity, stable quality, and good efficiency in the process of planting lettuce, indicative of an interesting design for space vegetable production.

Simple fabrication of pineapple root-like palladium-gold catalysts as the high-efficiency cathode in direct peroxide-peroxide fuel cells.

PubMed

Wang, Xin; Ye, Ke; Sun, Ce; Zhang, Hongyu; Zhu, Kai; Cheng, Kui; Wang, Guiling; Cao, Dianxue

2017-07-15

Pd-Au/TiC electrodes with various three-dimensional structures are obtained by the pulsed potential electro-deposition in PdCl 2 /HAuCl 4 electrolytes. The morphologies of Pd-Au/TiC composite catalysts are significantly dependent on the component of deposited solutions. The surface appearance of Pd-Au catalysts changes from rime-shaped structure, to feather-like construction, then to pineapple root-like structure and finally to flower-like configuration with the increase of PdCl 2 content in electrolytes. These particular three-dimensional structures may be very suitable for H 2 O 2 electro-reduction, which assures a high utilization of Pd-Au catalysts and provides a large specific surface area. The electro-catalytic activities of H 2 O 2 reduction on the Pd-Au/TiC electrodes improve as increasing the Pd content in Pd-Au alloy catalysts. The pineapple root-like Pd 5 Au 1 /TiC electrode reveals remarkably excellent electrochemical property and desirable stability for catalyzing H 2 O 2 reduction in acid media. The direct peroxide-peroxide fuel cells with a 10 cm 3 min -1 flow rate display the open circuit voltage (OCV) of 0.85V and the peak power density of 56.5mWcm -2 at 155mAcm -2 with desirable cell stability, which is much higher than those previously reported. Copyright © 2017 Elsevier Inc. All rights reserved.

The Potent Respiratory System of Osedax mucofloris (Siboglinidae, Annelida) - A Prerequisite for the Origin of Bone-Eating Osedax?

PubMed Central

Huusgaard, Randi S.; Vismann, Bent; Kühl, Michael; Macnaugton, Martin; Colmander, Veronica; Rouse, Greg W.; Glover, Adrian G.; Dahlgren, Thomas; Worsaae, Katrine

2012-01-01

Members of the conspicuous bone-eating genus, Osedax, are widely distributed on whale falls in the Pacific and Atlantic Oceans. These gutless annelids contain endosymbiotic heterotrophic bacteria in a branching root system embedded in the bones of vertebrates, whereas a trunk and anterior palps extend into the surrounding water. The unique life style within a bone environment is challenged by the high bacterial activity on, and within, the bone matrix possibly causing O2 depletion, and build-up of potentially toxic sulphide. We measured the O2 distribution around embedded Osedax and showed that the bone microenvironment is anoxic. Morphological studies showed that ventilation mechanisms in Osedax are restricted to the anterior palps, which are optimized for high O2 uptake by possessing a large surface area, large surface to volume ratio, and short diffusion distances. The blood vascular system comprises large vessels in the trunk, which facilitate an ample supply of oxygenated blood from the anterior crown to a highly vascularised root structure. Respirometry studies of O. mucofloris showed a high O2 consumption that exceeded the average O2 consumption of a broad line of resting annelids without endosymbionts. We regard this combination of features of the respiratory system of O. mucofloris as an adaptation to their unique nutrition strategy with roots embedded in anoxic bones and elevated O2 demand due to aerobic heterotrophic endosymbionts. PMID:22558289

Morphological Changes Of The Root Surface And Fracture Resistance After Treatment Of Root Fracture By CO2 Laser And Glass Ionomer Or Mineral Trioxide Aggregates

NASA Astrophysics Data System (ADS)

Badr, Y. A.; Abd El-Gawad, L. M.; Ghaith, M. E.

2009-09-01

This in vitro study evaluates the morphological changes of the root surface and fracture resistance after treatment of root cracks by CO2 laser and glass Ionomer or mineral trioxide aggregates (MTA). Fifty freshly extracted human maxillary central incisor teeth with similar dimension were selected. Crowns were sectioned at the cemento-enamel junction, and the lengths of the roots were adjusted to 13 mm. A longitudinal groove with a dimension of 1×5 mm2 and a depth of 1.5 mm was prepared by a high speed fissure bur on the labial surface of the root. The roots were divided into 5 groups: the 10 root grooves in group 1 were remained unfilled and were used as a control group. The 10 root grooves in group 2 were filled with glass Ionomer, 10 root grooves in group 3 were filled with MTA, the 10 root grooves in group 4 were filled with glass Ionomer and irradiated by CO2 laser and the 10 root grooves in group 5 were filled with MTA and irradiated with CO2 laser. Scanning electron microscopy was performed for two samples in each group. Tests for fracture strength were performed using a universal testing machine and a round tip of a diameter of 4 mm. The force was applied vertically with a constant speed of 1 mm min 1. For each root, the force at the time of fracture was recorded in Newtons. Results were evaluated statistically with ANOVA and Turkey's Honestly Significant Difference (HSD) tests. SEM micrographs revealed that the melted masses and the plate-like crystals formed a tight Chemical bond between the cementum and glass Ionomer and melted masses and globular like structure between cementum and MTA. The mean fracture resistance was the maximum fracture resistance in group 5 (810.8 N). Glass Ionomer and MTA with the help of CO2 laser can be an alternative to the treatment of tooth crack or fracture. CO2 laser increase the resistance of the teeth to fracture.

Using Flux Site Observations to Calibrate Root System Architecture Stencils for Water Uptake of Plant Functional Types in Land Surface Models.

NASA Astrophysics Data System (ADS)

Bouda, M.

2017-12-01

Root system architecture (RSA) can significantly affect plant access to water, total transpiration, as well as its partitioning by soil depth, with implications for surface heat, water, and carbon budgets. Despite recent advances in land surface model (LSM) descriptions of plant hydraulics, RSA has not been included because of its three-dimensional complexity, which makes RSA modelling generally too computationally costly. This work builds upon the recently introduced "RSA stencil," a process-based 1D layered model that captures the dynamic shifts in water potential gradients of 3D RSA in response to heterogeneous soil moisture profiles. In validations using root systems calibrated to the rooting profiles of four plant functional types (PFT) of the Community Land Model, the RSA stencil predicts plant water potentials within 2% of the outputs of full 3D models, despite its trivial computational cost. In transient simulations, the RSA stencil yields improved predictions of water uptake and soil moisture profiles compared to a 1D model based on root fraction alone. Here I show how the RSA stencil can be calibrated to time-series observations of soil moisture and transpiration to yield a water uptake PFT definition for use in terrestrial models. This model-data integration exercise aims to improve LSM predictions of soil moisture dynamics and, under water-limiting conditions, surface fluxes. These improvements can be expected to significantly impact predictions of downstream variables, including surface fluxes, climate-vegetation feedbacks and soil nutrient cycling.

Graviresponsiveness of surgically altered primary roots of Zea mays

NASA Technical Reports Server (NTRS)

Maimon, E.; Moore, R.

1991-01-01

We examined the gravitropic responses of surgically altered primary roots of Zea mays to determine the route by which gravitropic inhibitors move from the root tip to the elongating zone. Horizontally oriented roots, from which a 1-mm-wide girdle of epidermis plus 2-10 layers of cortex were removed from the apex of the elongating zone, curve downward. However, curvature occurred only apical to the girdle. Filling the girdle with mucilage-like material transmits curvature beyond the girdle. Vertically oriented roots with a half-girdle' (i.e. the epidermis and 2-10 layers of the cortex removed from half of the circumference of the apex of the elongating zone) curve away from the girdle. Inserting the half-girdle at the base of the elongating zone induces curvature towards the girdle. Filling the half-circumference girdles with mucilage-like material reduced curvature significantly. Stripping the epidermis and outer 2-5 layers of cortex from the terminal 1.5 cm of one side of a primary root induces curvature towards the cut, irrespective of the root's orientation to gravity. This effect is not due to desiccation since treated roots submerged in water also curved towards their cut surface. Coating a root's cut surface with a mucilage-like substance minimizes curvature. These results suggest that the outer cell-layers of the root, especially the epidermis, play an important role in root gravicurvature, and the gravitropic signals emanating from the root tip can move apoplastically through mucilage.

Compensatory Root Water Uptake of Overlapping Root Systems

NASA Astrophysics Data System (ADS)

Agee, E.; Ivanov, V. Y.; He, L.; Bisht, G.; Shahbaz, P.; Fatichi, S.; Gough, C. M.; Couvreur, V.; Matheny, A. M.; Bohrer, G.

2015-12-01

Land-surface models use simplified representations of root water uptake based on biomass distributions and empirical functions that constrain water uptake during unfavorable soil moisture conditions. These models fail to capture the observed hydraulic plasticity that allows plants to regulate root hydraulic conductivity and zones of active uptake based on local gradients. Recent developments in root water uptake modeling have sought to increase its mechanistic representation by bridging the gap between physically based microscopic models and computationally feasible macroscopic approaches. It remains to be demonstrated whether bulk parameterization of microscale characteristics (e.g., root system morphology and root conductivity) can improve process representation at the ecosystem scale. We employ the Couvreur method of microscopic uptake to yield macroscopic representation in a coupled soil-root model. Using a modified version of the PFLOTRAN model, which represents the 3-D physics of variably saturated soil, we model a one-hectare temperate forest stand under natural and synthetic climatic forcing. Our results show that as shallow soil layers dry, uptake at the tree and stand level shift to deeper soil layers, allowing the transpiration stream demanded by the atmosphere. We assess the potential capacity of the model to capture compensatory root water uptake. Further, the hydraulic plasticity of the root system is demonstrated by the quick response of uptake to rainfall pulses. These initial results indicate a promising direction for land surface models in which significant three-dimensional information from large root systems can be feasibly integrated into the forest scale simulations of root water uptake.

Engineering assessment of low-level liquid waste disposal caisson locations at the 618-11 Burial Grounds

DOE Office of Scientific and Technical Information (OSTI.GOV)

Phillips, S.J.; Fischer, D.D.; Crawford, R.C.

1982-06-01

Rockwell Hanford Operations is currently involved in an extensive effort to perform interim ground surface stabilization activities at retired low-level waste burial grounds located at the Hanford Site, Richland, Washington. The principal objective of these activities is to promote increased occupational and radiological safety at burial grounds. Interim stabilization activities include: (1) load testing (traversing burial ground surfaces with heavy equipment to promote incipient collapse of void spaces within the disposal structure and overburden), (2) barrier placement (placement of a {ge} 0.6 m soil barrier over existing overburden), and (3) revegetation (establishment of shallow rooted vegetation on the barrier tomore » mitigate deep rooted plant growth and to reduce erosion). Low-level waste disposal caissons were used in 300 Area Burial Grounds as internment structures for containerized liquid wastes. These caissons, by virtue of their contents, design and methods of closure, require long-term performance evaluation. As an initial activity to evaluate long-term performance, the accurate location of these structures is required. This topical report summarizes engineering activities used to locate caissons in the subsurface environment at the Burial Ground. Activities were conducted to locate caissons during surface stabilization activities. The surface locations were marked, photographed, and recorded on an as built engineering drawing. The recorded location of these caissons will augment long-term observations of confinement structure and engineered surface barrier performance. In addition, accurate caisson location will minimize occupational risk during monitoring and observation activities periodically conducted at the burial ground.« less

Enamel pearls in permanent dentition: case report and micro-CT evaluation

PubMed Central

Versiani, MA; Cristescu, RC; Pécora, JD; de Sousa-Neto, MD

2013-01-01

Objectives: To investigate the frequency, position, number and morphology of enamel pearls (EPs) using micro-CT (µCT) and to report a case of an EP mimicking an endodontic–periodontic lesion. Methods: Cone beam CT (CBCT) was performed in a patient to evaluate a radio-opaque nodule observed on the left maxillary first molar during the radiographic examination. Additionally, 23 EPs were evaluated regarding frequency, position, number and morphology by means of µCT. The results were statistically compared using the Student’s t-test for independent samples. Results: 1 pearl was presented in 13 specimens, while 5 specimens presented 2 pearls. The most frequent location of the EPs was the furcation between the disto-buccal and the palatal roots of the maxillary molars. Overall, the mean major diameter, volume and surface area were 1.98 ± 0.85 mm, 1.76 ± 1.36 mm3 and 11.40 ± 7.59 mm2, respectively, with no statistical difference between maxillary second and third molars (p > 0.05). In the case report, CBCT revealed an EP between the disto-buccal and the palatal roots of the maxillary first left molar associated with advanced localized periodontitis. The tooth was referred for extraction. Conclusions: EPs, located generally in the furcation area, were observed in 0.74% of the sample. The majority was an enamel–dentin pearl type and no difference was found in maxillary second and third molars regarding diameter, volume and surface area of the pearls. In this report, the EP mimicked an endodontic–periodontic lesion and was a secondary aetiological factor in the periodontal breakdown. PMID:23520396

The effect of complete radial lateral meniscus posterior root tear on the knee contact mechanics: a finite element analysis.

PubMed

Bao, H R C; Zhu, D; Gong, H; Gu, G S

2013-03-01

In recent years, with technological advances in arthroscopy and magnetic resonance imaging and improved biomechanical studies of the meniscus, there has been some progress in the diagnosis and treatment of injuries to the roots of the meniscus. However, the biomechanical effect of posterior lateral meniscus root tears on the knee has not yet become clear. The purpose of this study was to determine the effect of a complete radial posterior lateral meniscus root tear on the knee contact mechanics and the function of the posterior meniscofemoral ligament on the knee with tear in the posterior root of lateral meniscus. A finite element model of the knee was developed to simulate different cases for intact knee, a complete radial posterior lateral meniscus root tear, a complete radial posterior lateral meniscus root tear with posterior meniscofemoral ligament deficiency, and total meniscectomy of the lateral meniscus. A compressive load of 1000 N was applied in all cases to calculate contact areas, contact pressure, and meniscal displacements. The complete radial posterior lateral meniscus root tear decreased the contact area and increased the contact pressure on the lateral compartment under compressive load. We also found a decreased contact area and increased contact pressure in the medial compartment, but it was not obvious compared to the lateral compartment. The lateral meniscus was radially displaced by compressive load after a complete radial posterior lateral meniscus root tear, and the displacement took place mainly in the body and posterior horn of lateral meniscus. There were further decrease in contact area and increases in contact pressure and raidial displacement of the lateral meniscus in the case of the complete posterior lateral meniscus root tear in combination with posterior meniscofemoral ligament deficiency. Complete radial posterior lateral meniscus root tear is not functionally equivalent to total meniscectomy. The posterior root torn lateral meniscus continues to provide some load transmission and distribution functions across the joint. The posterior meniscofemoral ligament prevents excessive radial displacement of the posterior root torn lateral meniscus and assists the torn lateral meniscus in transmitting a certain amount of stress in the lateral compartment.

On the use of antibiotics to reduce rhizoplane microbial populations in root physiology and ecology investigations

NASA Technical Reports Server (NTRS)

Smart, D. R.; Ferro, A.; Ritchie, K.; Bugbee, B. G.

1995-01-01

No straightforward method exists for separating the proportion of ion exchange and respiration due to rhizoplane microbial organisms from that of root ion exchange and respiration. We examined several antibiotics that might be used for the temporary elimination of rhizoplane bacteria from hydroponically grown wheat roots (Triticum aestivum cv. Veery 10). Each antibiotic was tested for herbicidal activity and plate counts were used to enumerate bacteria and evaluate antibiotic kinetics. Only lactam antibiotics (penicillins and cephalosporins) did not reduce wheat growth rates. Aminoglycosides, the pyrimidine trimethoprim, colistin and rifampicin reduced growth rates substantially. Antibiotics acted slowly, with maximum reductions in rhizoplane bacteria occurring after more than 48 h of exposure. Combinations of nonphytotoxic antibiotics reduced platable rhizoplane bacteria by as much as 98%; however, this was generally a reduction from about 10(9) to 10(6) colony forming units per gram of dry root mass, so that many viable bacteria remained on root surfaces. We present evidence which suggests that insufficient bacterial biomass exists on root surfaces of nonstressed plants grown under well-aerated conditions to quantitatively interfere with root nitrogen absorption measurements.

[Three-dimensional evaluation of condylar morphology remodeling after orthognathic surgery in mandibular retrognathism by cone-beam computed tomography].

PubMed

Chen, Shuo; Liu, Xiao-jing; Li, Zi-li; Liang, Cheng; Wang, Xiao-xia; Fu, Kai-yuan; Yi, Biao

2015-08-18

To evaluate the effect of orthognathic surgery on condylar morphology changes by comparing three-dimension surface reconstructions of condyles using cone-beam computed tomography (CBCT) data. In the study, 18 patients with mandible retrognathism deformities were included and CBCT data of 36 temporomandibular joints were collected before surgery and 12 months after surgery. Condyles were reconstructed and superimposed pre- and post-operatively to compare the changes of condylar surfaces. One-sample t test and χ2 test were performed for the analysis of three-dimension metric measurement and condylar head remodeling signs. P<0.05 was considered significant. The root-mean-square (RMS) of condylar surface changes before and after the surgery was (0.37±0.11) mm, which was significant statistically (P<0.05). The distribution of condylar remodeling signs showed significant difference (P<0.05). Bone resorption occurred predominantly in the posterior area of condylar head and bone formation occurred mainly in the anterior area. Three-dimension superimposition method based on CBCT data showed that condylar morphology had undergone remodeling after mandibular advancement.

Surface soil root response to season of repeated fire in a young longleaf pine plantation

Treesearch

Mary Anne Sword Sayer; James D. Haywood

2012-01-01

The potential exists for interaction between naturally high soil bulk density and low soil water content to create root-growth limiting soil strengths. This problem is commonly remedied by soil structural attributes, old root channels and other perturbations, and periods of wetness during which soil strength is favorable for root elongation. Because the application and...

The inflow of Cs-137 in soil with root litter and root exudates of Scots pine

NASA Astrophysics Data System (ADS)

Shcheglov, Alexey; Tsvetnova, Olga; Popova, Evgenia

2017-04-01

In the model experiment on evaluation of Cs-137 inflow in the soil with litter of roots and woody plants root exudates on the example of soil and water cultures of Scots pine (Pinus sylvestris L.) was shown, that through 45 days after the deposit Cs-137 solution on pine needles (specific activity of solution was 3.718*106 Bk) of the radionuclide in all components of model systems has increased significantly: needles, small branches and trunk by Cs-137 surface contamination during the experiment; roots as a result of the internal distribution of the radionuclide in the plant; soil and soil solution due to the of receipt Cs-137 in the composition of root exudates and root litter. Over 99% of the total reserve of Cs-137 accumulated in the components of the soil and water systems, accounted for bodies subjected to external pollution (needles and small branches) and <0.5% - on the soil / soil solution, haven't been subjected to surface contamination. At the same contamination of soil and soil solution by Cs-137 in the model experiment more than a> 99.9% was due to root exudates

Histologic evaluation of autogenous connective tissue and acellular dermal matrix grafts in humans.

PubMed

Cummings, Lewis C; Kaldahl, Wayne B; Allen, Edward P

2005-02-01

The clinical success of root coverage with autogenous connective tissue (CT) or acellular dermal matrix (ADM) has been well documented. However, limited histological results of CT grafts have been reported, and a case report of a human block section has been published documenting an ADM graft. The purpose of this study is to document the histological results of CT grafts, ADM grafts, and coronally advanced flaps to cover denuded roots in humans. This study included four patients previously treatment planned for extractions of three or more anterior teeth. Three teeth in each patient were selected and randomly designated to receive either a CT or ADM graft beneath a coronally advanced flap (tests) or coronally advanced flap alone (control). Six months postoperatively block section extractions were performed and the teeth processed for histologic evaluation with hematoxylin-eosin and Verhoeff's stains. Histologically, both the CT and ADM were well incorporated within the recipient tissues. New fibroblasts, vascular elements, and collagen were present throughout the ADM, while retention of the transplanted elastic fibers was apparent. No effect on the keratinization or connective tissue organization of the overlying alveolar mucosa was evident with either graft. For both materials, areas of cemental deposition were present within the root notches, the alveolar bone was essentially unaffected, and the attachments to the root surfaces were similar. Although CT and ADM have a slightly different histological appearance, both can successfully be used to cover denuded roots with similar attachments and no adverse healing.

Methylboronic acid fertilization alleviates boron deficiency symptoms in Arabidopsis thaliana.

PubMed

Duran, Catherine; Arce-Johnson, Patricio; Aquea, Felipe

2018-07-01

Our results showed that methylboronic acid is capable of alleviating boron deficiency, enhancing plant growth, and is less toxic than boric acid at higher concentrations. Boron is an essential plant micronutrient and its deficiency occurs in several regions globally, resulting in impaired plant growth. Boron fertilization is a common agricultural practice, but the action range of boron is narrow, sharply transitioning from deficiency to toxicity. Boric acid (BA) is the most common chemical form used in agriculture. In this work, we describe that methylboronic acid (MBA) is capable of alleviating boron deficiency in Arabidopsis. MBA is a boronic acid, but does not naturally occur in soils, necessitating synthesis. Other boronic acids have been described as boron competitors in plants, inhibiting auxin biosynthesis and root development. MBA is more water-soluble than BA and delivers the same amount of boron per molecule. We observed that Arabidopsis seedlings grown in the presence of MBA presented higher numbers of lateral roots and greater main root length compared to plants grown in BA. In addition, root hair length and leaf surface area were increased using MBA as a boron fertilizer. Finally, MBA was less toxic than BA at high concentrations, producing a slight reduction in the main root length but no decrease in total chlorophyll. Our results open a new opportunity to explore the use of a synthetic form of boron in agriculture, providing a tool for future research for plant nutrition.

Release from or through a wax matrix system. I. Basic release properties of the wax matrix system.

PubMed

Yonezawa, Y; Ishida, S; Sunada, H

2001-11-01

Release properties from a wax matrix tablet was examined. To obtain basic release properties, the wax matrix tablet was prepared from a physical mixture of drug and wax powder (hydrogenated caster oil) at a fixed mixing ratio. Properties of release from the single flat-faced surface or curved side surface of the wax matrix tablet were examined. The applicability of the square-root time law and of Higuchi equations was confirmed. The release rate constant obtained as g/min(1/2) changed with the release direction. However, the release rate constant obtained as g/cm2 x min(1/2) was almost the same. Hence it was suggested that the release property was almost the same and the wax matrix structure was uniform independent of release surface or direction at a fixed mixing ratio. However, these equations could not explain the entire release process. The applicability of a semilogarithmic equation was not as good compared with the square-root time law or Higuchi equation. However, it was revealed that the semilogarithmic equation was available to simulate the entire release process, even though the fit was somewhat poor. Hence it was suggested that the semilogarithmic equation was sufficient to describe the release process. The release rate constant was varied with release direction. However, these release rate constants were expressed by a function of the effective surface area and initial amount, independent of the release direction.

Aortic root dilation in kidney transplant recipients.

PubMed

Obremska, Marta; Boratyńska, Maria; Szymczak, Maciej; Zyśko, Dorota; Płonek, Tomasz; Goździk, Anna; Klinger, Marian

2018-05-30

INTRODUCTION Aortic root (AoR) dilation is associated with cardiac damage and higher cardiovascular risk. Cardiovascular disease is the most common cause of death in patients after kidney transplantation (KTx ). OBJECTIVES The aim of this study was to assess the prevalence of enlarged AoR diameter in KTx recipients. Patients with bicuspid aortic valve, significant valvular disease, or evidence of connective tissue disorder were excluded. PATIENTS AND METHODS A total of 87 KTx recipients were divided into 2 groups depending on immunosuppressive regimen: 41 patients receiving mammalian target of rapamycin inhibitors (mTORi) and 46 patients treated with calcineurin inhibitors (CNIs). In all patients, echocardiography was performed, laboratory and clinical markers of cardiovascular risk were assessed, and the AoR diameter was calculated. RESULTS There were no differences between groups in age, sex, body surface area, body mass index, frequency of diabetes, hypertension, dyslipidemia, time after replacement therapy, creatinine levels, and estimated glomerular filtration rate. In the CNI group, the observed and calculated AoR diameters were similar (P = 0.8). In the mTORi group, the observed AoR diameter was higher than the calculated one (P = 0.002). The concentric and eccentric left ventricular hypertrophy was similar in both groups (P = 0.12 and P = 0.69, respectively). In the stepwise regression analysis, the AoR diameter was associated with body surface area and mTORi treatment. CONCLUSIONS KTx recipients have a high prevalence of AoR dilation. Immunosuppressive regimen based on mTORi increases the incidence of AoR enlargement.

Water resources of the Root River watershed, southeastern Minnesota

USGS Publications Warehouse

Broussard, W.L.; Farrell, D.F.; Anderson, H.W.; Felsheim, P.E.

1975-01-01

This Hydrologic Atlas is one of a series describing the 39 watersheds in Minnesota. The Root River watershed includes Houston, Winona, and parts of the surrounding counties. The 2 ,570 square miles in the watershed varies from gently rolling prairie in the west to an area of plateaus separated by valleys deeply incised into bedrock in the north and east. The average annual water budget for 30 years shows 29.5 inches of precipitation, 7.2 inches of surface runoff, and 22.3 inches of evapotranspiration. Water use in millions of gallons for 1970 was established at 7,310 of ground water and 6,700 of surface water. Domestic supplies accounted for less than one fourth and thermoelectric power for about one half of the total use. All 33 municipalities use ground water from bedrock aquifers, and 21 of those obtain at least part of their supply from the Prairie du Chien-Jordan aquifer. Many private domestic wells are completed in a shallow limestone aquifer. The ground-water system is recharged primarily by infiltrating precipitation in upland areas Ground-water movement is indicated by water-table and potentiometric maps and section diagrams. Water from bedrock and glacial aquifers generally is of acceptable quality for domestic use, dissolved solids generally less than 400 mg/liter very hard , and locally high iron content. The Mount Simon-Red clastics aquifer is locally saline. Runoff is greatest during the spring when snowmelt occurs and the soils are generally saturated.

Phytoremediation of soils contaminated by cadmium

NASA Astrophysics Data System (ADS)

Watai, H.; Miyazaki, T.; Fujikawa, T.; Mizoguchi, M.

2004-12-01

Phytoremediation is a technique to clean up soils contaminated with heavy metals. Advantages of this method are that (1) This technique is suitable to cleanup soils slightly contaminated with heavy metals in relatively wide area. (2) The expense for clean up is lower than civil engineering techniques. (3) This method can remove heavy metals fundamentally from contaminated. (4) The heavy metals are able to recycle by ashing of plants. Many researches have been done on the phytoremediation up to now, but almost all these researches were devoted to clarify the phytoremediation from the view point of plants themselves. However, few efforts have been devoted to analyze the migrations of heavy metals in soils during the phytoremediation process. The objective of this study is to clarify the features of Cd migration when plant roots are absorbing Cd from the ambient soils. Especially, we focused on finding the Cd migration pattern by changing the soil condition such as plant growing periods, planting densities, and the initial Cd concentration in soils. We planted sunflowers in columns filled with Cd contaminated soils because sunflower is a well-known hyperaccumulator of Cd from soils. By cutting the shoots of plants at the soil surface, and by keeping the plant roots in the soils without disturbance, the Cd concentrations, moisture contents, pH distributions, EC distributions, and dry weight of residual roots in the soils were carefully analyzed. The experimental results showed that (1)The growth of the planted sunflowers were suffered by applying of Cd. (2)The decrease of suction was affected by water uptake by roots at the depth from 0 to 5 cm. Water contents with plants in soils decrease more than without plants. (3)Cd adsorption by roots was predominant within 5cm from soil surface. In addition, it was also shown that there was an optimal Cd concentration where Cd is most effectively adsorbed by the plant. In this experiment we found that 40 to 60 mg kg-1 was the optimal concentration. By a trial calculation, it was revealed that more than 30 times of planting-cultivating processes were needed to decrease the Cd concentration from 9.75 to 0.4 mg Cd kg-1. When the sunflower was not planted, Cd did not move in the soils even when the soil water the sunflower was planted, Cd in the soil moved toward the plant roots associating with the water uptake by the roots. This Cd movement may have enhanced by the secretion of organic acid from plant roots.

USE OF NATIVE PLANTS FOR REMEDIATION OF TRICHLOROETHYLENE: I. DECIDUOUS TREES.

PubMed

Strycharz, S; Newman, L

2009-02-01

Phytoremediation of trichloroethylene (TCE) can be accomplished using fast-growing, deep-rooting trees. The most commonly used tree for phytoremediation of TCE has been the hybrid poplar. This study looks at native southeastern trees of the United States as alternatives to the use of hybrid poplar. The use of native trees for phytoremediation allows for simultaneous restoration of contaminated sites. A 2-mo, greenhouse-based study was conducted to determine if sycamore (Plantanus L.), eastern cottonwood (Populus deltoides), sweetgum (Liquidambar styraciflua L.), and willow (Salix sachalinensis) trees possess the ability to degrade TCE by assessing TCE metabolite formation in the plant tissue. In addition to the metabolic capabilities of each tree species, growth parameters were measured including change in height, water usage, total fresh weight of each tissue type, and calculated total leaf surface area. Willow trees had the greatest increase in height among all trees tested; however, at higher concentrations TCE inhibits growth. Sycamore trees had the highest overall leaf surface area and total biomass, which correlated with sycamore trees also having the highest average water usage over the course of the experiment. Carbon tubes used to sample transpiration gases from sycamore, sweetgum, and cottonwood trees did not contain detectable levels of TCE. Tenex sample collection tubes used to sample willow trees during TCE exposure showed average TCE concentrations of up to 0.354 ng TCE cm -2 leaf tissue. All exposed trees contained TCE in the root, stem, and leaf tissues. The concentration of TCE remaining in tissues at the conclusion of the experiment varied, with the highest levels found in the roots and the lowest levels found in the leaves. Metabolites were also observed in different tissue types of all trees tested. The highest concentrations of trichloroacetic acid were observed in the leaves of the sycamore trees and cottonwood trees. Based on the growth parameters tested and the ability to metabolize TCE, sycamore and native cottonwood species are the best candidates for phytoremediation from this study.

Apices of maxillary premolars observed by swept source optical coherence tomography

NASA Astrophysics Data System (ADS)

Ebihara, Arata; Iino, Yoshiko; Yoshioka, Toshihiko; Hanada, Takahiro; Sunakawa, Mitsuhiro; Sumi, Yasunori; Suda, Hideaki

2015-02-01

Apicoectomy is performed for the management of apical periodontitis when orthograde root canal treatment is not possible or is ineffective. Prior to the surgery, cone beam computed tomography (CBCT) examination is often performed to evaluate the lesion and the adjacent tissues. During the surgical procedure, the root apex is resected and the resected surface is usually observed under dental operating microscope (DOM). However, it is difficult to evaluate the details and the subsurface structure of the root using CBCT and DOM. A new diagnostic system, swept source optical coherence tomography (SS-OCT), has been developed to observe the subsurface anatomical structure. The aim of this study was to observe resected apical root canals of human maxillary premolars using SS-OCT and compare the findings with those observed using CBCT and DOM. Six extracted human maxillary premolars were used. After microfocus computed tomography (Micro CT; for gold standard) and CBCT scanning of the root, 1 mm of the apex was cut perpendicular to the long axis of the tooth. Each resected surface was treated with EDTA, irrigated with saline solution, and stained with methylene blue dye. The resected surface was observed with DOM and SS-OCT. This sequence was repeated three times. The number of root canals was counted and statistically evaluated. There was no significant difference in the accuracy of detecting root canals among CBCT, DOM and SS-OCT (p > 0.05, Wilcoxon test). Because SS-OCT can be used in real time during surgery, it would be a useful tool for observing resected apical root canals.

Monitoring the colonization of sugarcane and rice plants by the endophytic diazotrophic bacterium Gluconacetobacter diazotrophicus marked with gfp and gusA reporter genes.

PubMed

Rouws, L F M; Meneses, C H S G; Guedes, H V; Vidal, M S; Baldani, J I; Schwab, S

2010-09-01

To evaluate the colonization process of sugarcane plantlets and hydroponically grown rice seedlings by Gluconacetobacter diazotrophicus strain PAL5 marked with the gusA and gfp reporter genes. Sugarcane plantlets inoculated in vitro with PAL5 carrying the gfp::gusA plasmid pHRGFPGUS did not present green fluorescence, but beta-glucuronidase (GUS)-stained bacteria could be observed inside sugarcane roots. To complement this existing inoculation methodology for micropropagated sugarcane with a more rapid colonization assay, we employed hydroponically grown gnotobiotic rice seedlings to study PAL5-plant interaction. PAL5 could be isolated from the root surface (10(8) CFU g(-1)) and from surface-disinfected root and stem tissues (10(4) CFU g(-1)) of inoculated plants, suggesting that PAL5 colonized the internal plant tissues. Light microscopy confirmed the presence of bacteria inside the root tissue. After inoculation of rice plantlets with PAL5 marked with the gfp plasmid pHRGFPTC, bright green fluorescent bacteria could be seen colonizing the rice root surface, mainly at the sites of lateral root emergence, at root caps and on root hairs. The plasmids pHRGFPGUS and pHRGFPTC are valid tools to mark PAL5 and monitor the colonization of micropropagated sugarcane and hydroponic rice seedlings. These tools are of use to: (i) study PAL5 mutants affected in bacteria-plant interactions, (ii) monitor plant colonization in real time and (iii) distinguish PAL5 from other bacteria during the study of mixed inoculants.

7 CFR 201.56-10 - Spurge family, Euphorbiaceae.

Code of Federal Regulations, 2011 CFR

2011-01-01

... dicot. (2) Food reserves: Cotyledons, which are thin and leaf-like; endosperm (fleshy food-storage... the cotyledons, endosperm, and epicotyl above the soil surface. (4) Root system: A primary root, with secondary roots usually developing within the test period. (b) Abnormal seedling description. (1) Cotyledons...

7 CFR 201.56-10 - Spurge family, Euphorbiaceae.

Code of Federal Regulations, 2010 CFR

2010-01-01

... dicot. (2) Food reserves: Cotyledons, which are thin and leaf-like; endosperm (fleshy food-storage... the cotyledons, endosperm, and epicotyl above the soil surface. (4) Root system: A primary root, with secondary roots usually developing within the test period. (b) Abnormal seedling description. (1) Cotyledons...

Measurement of Net Fluxes of Ammonium and Nitrate at the Surface of Barley Roots Using Ion-Selective Microelectrodes 1

PubMed Central

Henriksen, Gordon H.; Raman, D. Raj; Walker, Larry P.; Spanswick, Roger M.

1992-01-01

Net fluxes of NH4+ and NO3− into roots of 7-day-old barley (Hordeum vulgare L. cv Prato) seedlings varied both with position along the root axis and with time. These variations were not consistent between replicate plants; different roots showed unique temporal and spatial patterns of uptake. Axial scans of NH4+ and NO3− net fluxes were conducted along the apical 7 centimeters of seminal roots of intact barley seedlings in solution culture using ion-selective microelectrodes in the unstirred layer immediately external to the root surface. Theoretically derived relationships between uptake and concentration gradients, combined with experimental observations of the conditions existing in our experimental system, permitted evaluation of the contribution of bulk water flow to ion movement in the unstirred layer, as well as a measure of the spatial resolution of the microelectrode flux estimation technique. Finally, a method was adopted to assess the accuracy of this technique. PMID:16668947

Endophytic colonization of olive roots by the biocontrol strain Pseudomonas fluorescens PICF7.

PubMed

Prieto, Pilar; Mercado-Blanco, Jesús

2008-05-01

Confocal microscopy combined with three-dimensional olive root tissue sectioning was used to provide evidence of the endophytic behaviour of Pseudomonas fluorescens PICF7, an effective biocontrol strain against Verticillium wilt of olive. Two derivatives of the green fluorescent protein (GFP), the enhanced green and the red fluorescent proteins, have been used to visualize simultaneously two differently fluorescently tagged populations of P. fluorescens PICF7 within olive root tissues at the single cell level. The time-course of colonization events of olive roots cv. Arbequina by strain PICF7 and the localization of tagged bacteria within olive root tissues are described. First, bacteria rapidly colonized root surfaces and were predominantly found in the differentiation zone. Thereafter, microscopy observations showed that PICF7-tagged populations eventually disappeared from the root surface, and increasingly colonized inner root tissues. Localized and limited endophytic colonization by the introduced bacteria was observed over time. Fluorescent-tagged bacteria were always visualized in the intercellular spaces of the cortex region, and no colonization of the root xylem vessels was detected at any time. To the best of our knowledge, this is the first time this approach has been used to demonstrate endophytism of a biocontrol Pseudomonas spp. strain in a woody host such as olive using a nongnotobiotic system.