Sample records for salmonids volume xxii

  1. Proceedings of the XXII A.I.VE.LA. National Meeting

    NASA Astrophysics Data System (ADS)

    Primo Tomasini, Enrico

    2015-11-01

    A.I.VE.LA. - the Italian Association of Laser Velocimetry and non-invasive diagnostics - is a non-profit cultural association whose objective is to promote and support research in the field of non-contact or minimally invasive measurement techniques, particularly electromagnetic-based techniques and optical techniques. Through its Annual Meeting, AIVELA aims to create an active and stimulating forum where current research results and technical advances can be exchanged and the development of new systems for laboratory use, field testing and industrial application can be promoted. The techniques covered include Laser Doppler Anemometry - LDA, Phase Doppler Anemometry - PDA, Image Velocimetry - PIV, Flow visualization techniques, Spectroscopic measurement techniques (LIF, Raman, etc.), Laser Doppler Vibrometry - LDV, Speckle Pattern Interferometry - ESPI, Holographic techniques, Shearography, Digital Image Correlation - DIC, Moiré techniques, Structured light techniques, Infrared imaging, Photoelasticity, Image based measurement techniques, Ultrasonic sensing, Acoustic and Aeroacoustic measurements, etc. The first Annual Meeting was held back in October 1992 and since then there has been a large consensus among the research and scientific communities that the papers presented at the event are of a high scientific interest. The XXII AIVELA Annual Meeting was held at the Faculty of Engineering of University of Rome Tor Vergata on 15-16 December 2014 and was organised in collaboration with the International Master Courses in "Protection Against CBRNe Events". This volume contains a selection of the papers presented at the event. The detailed Programme of the Meeting can be found at: http://www.aivela.org/XXII_Convegno/index.html Trusting our Association and its initiatives will meet your interest, I wish to thank you in advance for your kind attention and hope to meet you soon at one of our events.

  2. Dielectronic satellite spectra of hydrogen-like titanium (Ti XXII)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bitter, M.; von Goeler, S.; Cohen, S.

    High resolution spectra of the Ly ..cap alpha../sub 1/ and Ly ..cap alpha../sub 2/ lines of hydrogenlike titanium, TiXXII, and the associated dielectronic satellites which are due to transitions 1snl-2pnl with n greater than or equal to 2, have been observed from tokamak discharges with auxiliary ion cyclotron heating (ICRH) with central electron temperatures of 2 keV and central electron densities of 8 x 10/sup 13/ cm/sup -3/ on the Princeton Large Torus (PLT). The data have been used for a detailed comparison with theoretical predictions based on the Z - expansion method and Hartree - Fock calculations. The resultsmore » obtained with the Z - expansion method are in excellent agreement with the observed spectral data except for minor discrepancies between the theoretical and experimental wavelengths of 0.0003 A for the n = 2 satellites and of 0.0001 A for the separation of the Ly ..cap alpha../sub 1/ and Ly ..cap alpha../sub 2/ lines. Very good agreement with the experimental data is also obtained for the results from the Hartree - Fock calculations though somewhat larger discrepancies (approx. = 0.0009 A) exist between experimental and theoretical wavelengths which are systematically too small. The observed spectra are used for diagnosis of the central ion and electron temperatures of the PLT discharges and for a measurement of the dielectronic recombination rate coefficient of TiXXII.« less

  3. Introduced northern pike consumption of salmonids in Southcentral Alaska

    USGS Publications Warehouse

    Sepulveda, Adam J.; Rutz, David S.; Dupuis, Aaron W; Shields, Patrick A; Dunker, Kristine J.

    2015-01-01

    The impacts of introduced northern pike (Esox lucius) on salmonid populations have attracted much attention because salmonids are popular subsistence, sport and commercial fish. Concern over the predatory effects of introduced pike on salmonids is especially high in Southcentral Alaska, where pike were illegally introduced to the Susitna River basin in the 1950s. We used pike abundance, growth, and diet estimates and bioenergetics models to characterise the realised and potential consumptive impacts that introduced pike (age 2 and older) have on salmonids in Alexander Creek, a tributary to the Susitna River. We found that juvenile salmonids were the dominant prey item in pike diets and that pike could consume up to 1.10 metric tons (realised consumption) and 1.66 metric tons (potential consumption) of juvenile salmonids in a summer. Age 3–4 pike had the highest per capita consumption of juvenile salmonids, and age 2 and age 3–4 pike had the highest overall consumption of juvenile salmonid biomass. Using historical data on Chinook salmon and pike potential consumption of juvenile salmonids, we found that pike consumption of juvenile salmonids may lead to collapsed salmon stocks in Alexander Creek. Taken together, our results indicate that pike consume a substantial biomass of juvenile salmonids in Alexander Creek and that coexistence of pike and salmon is unlikely without management actions to reduce or eliminate introduced pike.

  4. Food supplies of stream-dwelling salmonids

    USGS Publications Warehouse

    Wipfli, Mark S.

    2009-01-01

    Much is known about the importance of the physical characteristics of salmonid habitat in Alaska and the Pacific Northwest, with far less known about the food sources and trophic processes within these habitats, and the role they play in regulating salmonid productivity. Freshwater food webs supporting salmonids in Alaska rely heavily on nutrient, detritus and prey subsidies from both marine and terrestrial ecosystems. Adult salmon provide a massive input of marine biomass to riverine ecosystems each year when they spawn, die, and decompose, and are a critical food source for young salmon in late summer and fall; riparian forests provide terrestrial invertebrates to streams, which at times comprise over half of the food ingested by stream-resident salmonids; and up-slope, fishless headwater streams are a year-round source of invertebrates and detritus for fish downstream. The quantity of these food resources vary widely depending on source, season, and spatial position within a watershed. Terrestrial invertebrate inputs from riparian habitats are generally the most abundant food source in summer. Juvenile salmonids in streams consume roughly equal amounts of freshwater and terrestrially-derived invertebrates during most of the growing season, but ingest substantial amounts of marine resources (salmon eggs and decomposing salmon tissue) when these food items are present. Quantity, quality, and timing of food resources all appear to be important driving forces in aquatic food web dynamics, community nutrition, and salmonid growth and survival in riverine ecosystems.

  5. The Design and Analysis of Salmonid Tagging Studies in the Columbia Basin : Volume II: Experiment Salmonid Survival with Combined PIT-CWT Tagging.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Newman, Ken

    1997-06-01

    Experiment designs to estimate the effect of transportation on survival and return rates of Columbia River system salmonids are discussed along with statistical modeling techniques. Besides transportation, river flow and dam spill are necessary components in the design and analysis otherwise questions as to the effects of reservoir drawdowns and increased dam spill may never be satisfactorily answered. Four criteria for comparing different experiment designs are: (1) feasibility, (2) clarity of results, (3) scope of inference, and (4) time to learn. In this report, alternative designs for conducting experimental manipulations of smolt tagging studies to study effects of river operationsmore » such as flow levels, spill fractions, and transporting outmigrating salmonids around dams in the Columbia River system are presented. The principles of study design discussed in this report have broad implications for the many studies proposed to investigate both smolt and adult survival relationships. The concepts are illustrated for the case of the design and analysis of smolt transportation experiments. The merits of proposed transportation studies should be measured relative to these principles of proper statistical design and analysis.« less

  6. Introduced northern pike predation on salmonids in southcentral Alaska

    USGS Publications Warehouse

    Sepulveda, Adam J.; Rutz, David S.; Ivey, Sam S.; Dunker, Kristine J.; Gross, Jackson A.

    2013-01-01

    Northern pike (Esox lucius) are opportunistic predators that can switch to alternative prey species after preferred prey have declined. This trophic adaptability allows invasive pike to have negative effects on aquatic food webs. In Southcentral Alaska, invasive pike are a substantial concern because they have spread to important spawning and rearing habitat for salmonids and are hypothesised to be responsible for recent salmonid declines. We described the relative importance of salmonids and other prey species to pike diets in the Deshka River and Alexander Creek in Southcentral Alaska. Salmonids were once abundant in both rivers, but they are now rare in Alexander Creek. In the Deshka River, we found that juvenile Chinook salmon (Oncorhynchus tshawytscha) and coho salmon (O. kisutch) dominated pike diets and that small pike consumed more of these salmonids than large pike. In Alexander Creek, pike diets reflected the distribution of spawning salmonids, which decrease with distance upstream. Although salmonids dominated pike diets in the lowest reach of the stream, Arctic lamprey (Lampetra camtschatica) and slimy sculpin (Cottus cognatus) dominated pike diets in the middle and upper reaches. In both rivers, pike density did not influence diet and pike consumed smaller prey items than predicted by their gape-width. Our data suggest that (1) juvenile salmonids are a dominant prey item for pike, (2) small pike are the primary consumers of juvenile salmonids and (3) pike consume other native fish species when juvenile salmonids are less abundant. Implications of this trophic adaptability are that invasive pike can continue to increase while driving multiple species to low abundance.

  7. Functional Analysis of All Salmonid Genomes (FAASG): an international initiative supporting future salmonid research, conservation and aquaculture

    USDA-ARS?s Scientific Manuscript database

    We describe an emerging initiative - the 'Functional Analysis of All Salmonid Genomes' (FAASG), which will leverage the extensive trait diversity that has evolved since a whole genome duplication event in the salmonid ancestor, to develop an integrative understanding of the functional genomic basis ...

  8. A Negotiated Agreement between Tacoma Community College Federation of Teachers and Tacoma Community College, District XXII.

    ERIC Educational Resources Information Center

    Tacoma Community Coll., WA.

    The collective bargaining agreement between the Tacoma Community College Federation of Teachers and Tacoma Community College, District XXII is presented. This contract, covering the period from September 1, 1988 to June 30, 1990, deals with the following topics: the purpose, definitions, and conditions of the agreement; union rights and…

  9. Conservation genomics: coming to a salmonid near you.

    PubMed

    Piccolo, J J

    2016-12-01

    Using the examples on hereditary and environmental factors affecting salmonid populations, this paper demonstrates that ecologists have long appreciated the importance of local adaptation and intraspecific diversity for salmonid conservation. Conservationists, however, need to embrace the genomics revolution and use new insights to improve salmonid management. At the same time, researchers must be forthcoming with the uses and limitations of genomics, and conservation must move forward in the face of scientific uncertainty. © 2016 The Fisheries Society of the British Isles.

  10. Demonstration of the salmonid humoral response to Renibacterium salmoninarum using a monoclonal antibody against salmonid immunoglobulin

    USGS Publications Warehouse

    Bartholomew, J.L.; Arkoosh , M.R.; Rohovec, J.S.

    1991-01-01

    The specificity of the antibody response of salmonids to Renibacterium salmoninarum antigens was demonstrated by western blotting techniques that utilized a monoclonal antibody against salmonid immunoglobulin. In this study, the specificity of the response in immunized chinook salmon Oncorhynchus tshawytschawas compared with the response in naturally infected chinook salmon and coho salmon O. kisutch, and immunized rabbits. The antibody response in immunized salmon and rabbits and the naturally infected fish was primarily against the 57–58kilodalton protein complex. In addition to recognizing these proteins in the extracellular fraction and whole-cell preparations, antibody from the immunized salmon and rabbits detected four proteins with lower molecular masses. Western blotting techniques allow identification of the specific antigens recognized and are a useful tool for comparing the immunogenicity of different R. salmoninarumpreparations. Immunofluorescent techniques with whole bacteria were less sensitive than western blotting in detecting salmonid anti-R. salmoninarumantibody.

  11. A mathematical model of salmonid spawning habitat

    Treesearch

    Robert N. Havis; Carlos V. Alonzo; Keith E Woeste; Russell F. Thurow

    1993-01-01

    A simulation model [Salmonid Spawning Analysis Model (SSAM)I was developed as a management tool to evaluate the relative impacts of stream sediment load and water temperature on salmonid egg survival. The model is usefi.il for estimating acceptable sediment loads to spawning habitat that may result from upland development, such as logging and agriculture. Software in...

  12. Functional Annotation of All Salmonid Genomes (FAASG): an international initiative supporting future salmonid research, conservation and aquaculture.

    PubMed

    Macqueen, Daniel J; Primmer, Craig R; Houston, Ross D; Nowak, Barbara F; Bernatchez, Louis; Bergseth, Steinar; Davidson, William S; Gallardo-Escárate, Cristian; Goldammer, Tom; Guiguen, Yann; Iturra, Patricia; Kijas, James W; Koop, Ben F; Lien, Sigbjørn; Maass, Alejandro; Martin, Samuel A M; McGinnity, Philip; Montecino, Martin; Naish, Kerry A; Nichols, Krista M; Ólafsson, Kristinn; Omholt, Stig W; Palti, Yniv; Plastow, Graham S; Rexroad, Caird E; Rise, Matthew L; Ritchie, Rachael J; Sandve, Simen R; Schulte, Patricia M; Tello, Alfredo; Vidal, Rodrigo; Vik, Jon Olav; Wargelius, Anna; Yáñez, José Manuel

    2017-06-27

    We describe an emerging initiative - the 'Functional Annotation of All Salmonid Genomes' (FAASG), which will leverage the extensive trait diversity that has evolved since a whole genome duplication event in the salmonid ancestor, to develop an integrative understanding of the functional genomic basis of phenotypic variation. The outcomes of FAASG will have diverse applications, ranging from improved understanding of genome evolution, to improving the efficiency and sustainability of aquaculture production, supporting the future of fundamental and applied research in an iconic fish lineage of major societal importance.

  13. The relationship between productivities of salmonids and forest stands in northern California watersheds

    USGS Publications Warehouse

    Frazey, S.L.; Wilzbach, M.A.

    2007-01-01

    Productivities of resident salmonids and upland and riporian forests in 22 small watersheds of coastal northern California were estimated and compared to determine whether: 1) upland site productivity predicted riparian site productivity; 2) either upland or riparian site productivity predicted salmonid productivity; and 3) other parameters explained more of the variance in salmonid productivity. Upland and riparian site productivities were estimated using Site Index values for redwood (Sequoia sempervirens) and red alder (Alnus rubra), respectively. Salmonid productivity was indexed by back-calculated length at age 1 of the largest individuals sampled and by total biomass. Upland and riparian site indices were correlated, but neither factor contributed to the best approximating models of salmonid productivity. Total salmonid biomass was best described by a positive relationship with drainage area. Length of dominant fish was best described by a positive relationship with percentage of hardwoods within riparian areas, which may result from nutrient and/or litter subsidies provided by red older. The inability of forest productivity to predict salmon productivity may reflect insufficient variation in independent variables, limitations of the indices, and the operation of other factors affecting salmonid production. The lack of an apparent relationship between upland conifer and salmonid productivity suggests that management of land for timber productivity and component streams for salmonid production in these sites will require separate, albeit integrated, management strategies.

  14. SINE sequences detect DNA fingerprints in salmonid fishes.

    PubMed

    Spruell, P; Thorgaard, G H

    1996-04-01

    DNA probes homologous to two previously described salmonid short interspersed nuclear elements (SINEs) detected DNA fingerprint patterns in 14 species of salmonid fishes. The probes showed more homology to some species than to others and little homology to three nonsalmonid fishes. The DNA fingerprint patterns derived from the SINE probes are individual-specific and inherited in a Mendelian manner. Probes derived from different regions of the same SINE detect only partially overlapping banding patterns, reflecting a more complex SINE structure than has been previously reported. Like the human Alu sequence, the SINEs found in salmonids could provide useful genetic markers and primer sites for PCR-based techniques. These elements may be more desirable for some applications than traditional DNA fingerprinting probes that detect tandemly repeated arrays.

  15. Physiological levels of testosterone kill salmonid leukocytes in vitro

    USGS Publications Warehouse

    Slater, C.H.; Schreck, C.B.

    1997-01-01

    Adult spring chinook salmon (Oncorhynchus tshawytscha) elaborate high plasma concentrations of testosterone during sexual maturation, and these levels of testosterone have been shown to reduce the salmonid immune response in vitro. Our search for the mechanism of testosterone's immunosuppressive action has led to the characterization of an androgen receptor in salmonid leukocytes. In the present study we examined the specific effects that testosterone had on salmonid leukocytes. Direct counts of viable leukocytes after incubation with and without physiological levels of testosterone demonstrate a significant loss of leukocytes in cultures exposed to testosterone. At least 5 days of contact with testosterone was required to produce significant immunosuppression and addition of a 'conditioned media' (supernatant from proliferating lymphocytes not exposed to testosterone) did not reverse the immunosuppressive effects of testosterone. These data lead us to conclude that testosterone may exert its immunosuppressive effects by direct action on salmonid leukocytes, through the androgen receptor described, and that this action leads to the death of a significant number of these leukocytes.

  16. Density, aggregation, and body size of northern pikeminnow preying on juvenile salmonids in a large river

    USGS Publications Warehouse

    Petersen, J.H.

    2001-01-01

    Predation by northern pikeminnow Ptychocheilus oregonensis on juvenile salmonids Oncorhynchus spp. occurred probably during brief feeding bouts since diets were either dominated by salmonids (>80% by weight), or contained other prey types and few salmonids (<5%). In samples where salmonids had been consumed, large rather than small predators were more likely to have captured salmonids. Transects with higher catch-per-unit of effort of predators also had higher incidences of salmonids in predator guts. Predators in two of three reservoir areas were distributed more contagiously if they had preyed recently on salmonids. Spatial and temporal patchiness of salmonid prey may be generating differences in local density, aggregation, and body size of their predators in this large river.

  17. Evolutionary consequences of habitat loss for Pacific anadromous salmonids

    PubMed Central

    McClure, Michelle M; Carlson, Stephanie M; Beechie, Timothy J; Pess, George R; Jorgensen, Jeffrey C; Sogard, Susan M; Sultan, Sonia E; Holzer, Damon M; Travis, Joseph; Sanderson, Beth L; Power, Mary E; Carmichael, Richard W

    2008-01-01

    Large portions of anadromous salmonid habitat in the western United States has been lost because of dams and other blockages. This loss has the potential to affect salmonid evolution through natural selection if the loss is biased, affecting certain types of habitat differentially, and if phenotypic traits correlated with those habitat types are heritable. Habitat loss can also affect salmonid evolution indirectly, by reducing genetic variation and changing its distribution within and among populations. In this paper, we compare the characteristics of lost habitats with currently accessible habitats and review the heritability of traits which show correlations with habitat/environmental gradients. We find that although there is some regional variation, inaccessible habitats tend to be higher in elevation, wetter and both warmer in the summer and colder in the winter than habitats currently available to anadromous salmonids. We present several case studies that demonstrate either a change in phenotypic or life history expression or an apparent reduction in genetic variation associated with habitat blockages. These results suggest that loss of habitat will alter evolutionary trajectories in salmonid populations and Evolutionarily Significant Units. Changes in both selective regime and standing genetic diversity might affect the ability of these taxa to respond to subsequent environmental perturbations. Both natural and anthropogenic and should be considered seriously in developing management and conservation strategies. PMID:25567633

  18. Population viability assessment of salmonids by using probabilistic networks

    Treesearch

    Danny C. Lee; Bruce E. Rieman

    1997-01-01

    Public agencies are being asked to quantitatively assess the impact of land management activities on sensitive populations of salmonids. To aid in these assessments, we developed a Bayesian viability assessment procedure (BayVAM) to help characterize land use risks to salmonids in the Pacific Northwest. This procedure incorporates a hybrid approach to viability...

  19. Linking Forests and Fish: The Relationship Between Productivities of Salmonids and Forest Stands in Northern California

    NASA Astrophysics Data System (ADS)

    Wilzbach, P.; Frazey, S.

    2005-05-01

    Productivities of resident salmonid populations, upland, and riparian areas in 25 small watersheds of coastal northern California were estimated and compared to determine if: 1) upland site productivity predicted riparian site productivity; 2) either upland or riparian site productivity predicted salmonid productivity; and 3) other parameters explained more of the variance in salmonid productivity than upland or riparian site productivity. Salmonid productivity was indexed by total salmonid biomass, length of age 1 fish, and percent habitat saturation. Upland and riparian site productivities were estimated using site indices for redwood (Sequoia sempervirens) and red alder (Alnus rubra), respectively. Upland and riparian site indices were correlated, but neither factor contributed to the best approximating models of salmonid biomass or fish length at age one. Salmonid biomass was best described by a positive relationship with drainage area, and length at age was best described by a positive relationship with percent of riparian hardwoods. Percent habitat saturation was not well described by any of the models constructed. Lack of a relationship between upland conifer and salmonid productivity suggests that management of land for timber productivity and component streams for salmonid production in these sites will require separate, albeit integrated, strategies.

  20. Sustaining salmonid populations: A caring understanding of naturalness of taxa

    USGS Publications Warehouse

    Nielsen, Jennifer L.; Regier, Henry A.; Knudsen, E. Eric

    2004-01-01

    Species of the family of Salmonidae occur naturally in Northern Hemisphere waters that remain clear and cool to cold in summer. For purposes of reproduction, salmonids generally behaviorally respond to the currents of streams and lakes in recently glaciated areas. For feeding and maturation, many larger species migrate into existing systems of large lakes, seas, and oceans. The subfamilies include Salmoninae, Coregoninae, and Thymallinae. In many locales and regions of the hemisphere, numerous species of these subfamilies evolved and self-organized into species flocks or taxocenes of bewildering complexity. For example, any individual species may play different or unique ecological roles in different taxocenes. The northern Pacific and Atlantic Ocean ecosystems, with their seas and tributaries, each contained a metacomplex of such taxocenes that, in their natural state some centuries ago, resembled each other but differed in many ways. Humans have valued all species of this family for subsistence, ceremonial, naturalist, gustatory, angling, and commercial reasons for centuries. Modern progressive humans (MPHs), whose industrial and commercial enterprises have gradually spread over this hemisphere in recent time, now affect aquatic ecosystems at all scales from local to global. These human effects mingle in complex ways that together induce uniquely natural salmonid taxocenes to disintegrate with the loss of species, including those groups least tolerant to human manipulations, but extending more recently to those taxa more adapted to anthropogenic change. As we leave the modern era, dominated by MPHs, will we find ways to live sustainably with salmonid taxocenes that still exhibit self-organizational integrity, or will only individual, isolated populations of salmonid species, derived from those most tolerant of MPHs, survive? To achieve future sustainability of salmonids, we suggest implementation of a search for intuitive knowledge based on faith in the wisdom of

  1. Review of the negative influences of non-native salmonids on native fish species

    USGS Publications Warehouse

    Turek, Kelly C.; Pegg, Mark A.; Pope, Kevin L.

    2013-01-01

    Non-native salmonids are often introduced into areas containing species of concern, yet a comprehensive overview of the short- and long-term consequences of these introductions is lacking in the Great Plains. Several authors have suggested that non-native salmonids negatively inflfluence species of concern. The objective of this paper is to review known interactions between non-native salmonids and native fifishes, with a focus on native species of concern. After an extensive search of the literature, it appears that in many cases non-native salmonids do negatively inflfl uence species of concern (e.g., reduce abundance and alter behavior) via different mechanisms (e.g., predation and competition). However, there are some instances in which introduced salmonids have had no perceived negative inflfl uence on native fifi shes. Unfortunately, the majority of the literature is circumstantial, and there is a need to experimentally manipulate these interactions.

  2. Salmonid whirling disease

    USGS Publications Warehouse

    Markiw, M.E.

    1992-01-01

    This paper provides the latest scientific and technical advances in the management of salmonid whirling disease caused by the myxosporean Myxobolus cerebralis (Syn. Myxosoma cerebralis). The complete life cycle of the parasite and the biology of the infective agent to fish, the actinosporean Triactinomyxon stage, are reviewed, and suggested procedures for detection, identification, and control of the disease are discussed. The paper offers sources of information and related reference materials of interest to fish culturists, fishery biologists, and students.

  3. Modeling Food Delivery Dynamics For Juvenile Salmonids Under Variable Flow Regimes

    NASA Astrophysics Data System (ADS)

    Harrison, L.; Utz, R.; Anderson, K.; Nisbet, R.

    2010-12-01

    Traditional approaches for assessing instream flow needs for salmonids have typically focused on the importance of physical habitat in determining fish habitat selection. This somewhat simplistic approach does not account for differences in food delivery rates to salmonids that arise due to spatial variability in river morphology, hydraulics and temporal variations in the flow regime. Explicitly linking how changes in the flow regime influences food delivery dynamics is an important step in advancing process-based bioenergetic models that seek to predict growth rates of salmonids across various life-stages. Here we investigate how food delivery rates for juvenile salmonids vary both spatially and with flow magnitude in a meandering reach of the Merced River, CA. We utilize a two-dimensional (2D) hydrodynamic model and discrete particle tracking algorithm to simulate invertebrate drift transport rates at baseflow and a near-bankfull discharge. Modeling results indicate that at baseflow, the maximum drift density occurs in the channel thalweg, while drift densities decrease towards the channel margins due to the process of organisms settling out of the drift. During high-flow events, typical of spring dam-releases, the invertebrate drift transport pathway follows a similar trajectory along the high velocity core and the drift concentrations are greatest in the channel centerline, though the zone of invertebrate transport occupies a greater fraction of the channel width. Based on invertebrate supply rates alone, feeding juvenile salmonids would be expected to be distributed down the channel centerline where the maximum predicted food delivery rates are located in this reach. However, flow velocities in these channel sections are beyond maximum sustainable swimming speeds for most juvenile salmonids. Our preliminary findings suggest that a lack of low velocity refuge may prevent juvenile salmonids from deriving energy from the areas with maximum drift density in this

  4. Influence of intermittent stream connectivity on water quality and salmonid survivorship.

    NASA Astrophysics Data System (ADS)

    Hildebrand, J.; Woelfle-Erskine, C. A.; Larsen, L.

    2014-12-01

    Anthropogenic stress and climate change are causing an increasing number of California streams to become intermittent and are driving earlier and more severe summertime drying. The extent to which emerging water conservation alternatives impact flows or habitat quality (e.g. temperature, DO) for salmonids remains poorly understood. Here, we investigate the proximal drivers of salmonid mortality over a range of connectivity conditions during summertime intermittency in Salmon Creek watershed, Sonoma County, CA. Through extensive sampling in paired subwatersheds over a period of two years, we tested the hypothesis that accumulation of readily bioavailable DOC in poorly flushed pools drives DO decline associated with loss of salmonids. We then traced the origin and flow pathways of DOC throughout the watershed using Parallel Factor Analysis (PARAFAC). We obtained samples for DOC and stable isotope analyses at monthly intervals from 20 piezometers and surface water in the study reaches and from private wells and springs distributed throughout the watersheds. We also obtained in situ DO, conductivity and pH readings within stream study reaches. We determined DOC quality by SUVA (specific UV absorbance) and fluorescence index. We calculated stream metabolism rates using the single station method. In pools instrumented with DO sensors, we compared changing DOC quality during the summer months to changes in DO concentrations and stream metabolism. Our results show that the duration of complete disconnection of pools during the summer months and stream metabolic rates are positively correlated with salmonid mortality. Furthermore, our results indicate that salmonid mortality is greatest in disconnected pools with low DOC fluorescence indices and high SUVA values, indicative of terrestrially derived DOC and little or no groundwater inflow. Conversely low salmonid mortality was found in disconnected pools with high fluorescence index and low SUVA, indicative of microbially

  5. Feeding of predaceous fishes on out-migrating juvenile-salmonids in John Day Reservoir, Columbia River

    USGS Publications Warehouse

    Poe, Thomas P.; Hansel, Hal C.; Vigg, S.; Palmer, D.E.; Prendergast, L.A.

    1991-01-01

    Diets of northern squawfish Ptychocheilus oregonensis, smallmouth bass Micropterus dolomieu, walleye Stizostedion vitreum, and channel catfish Ictalurus punctatus from John Day Reservoir were examined to determine the extent of predation on juvenile salmonids during seaward migrations of the salmonids during April–August 1983–1986. Juvenile Pacific salmon Oncorhynchus spp. and steelhead O. mykiss were the most important food group (by weight) of northern squawfish – about 67% – but made up smaller proportions of the food of the other predators: channel catfish, 33%; walleyes, 14%; smallmouth bass, 4%. Seasonal changes in diets indicated that northern squawfish preferred juvenile salmonids in May and August (generally the peak period of salmonid out-migration), and switched to prickly sculpin Cottus asper when numbers of juvenile salmonids declined; walleyes and smallmouth bass showed a preference only for prickly sculpin among the prey fishes analyzed. As judged by dietary composition and prey selectivity, the northern squawfish was the major fish predator on juvenile salmonids in the reservoir; channel catfish also were important predators in the upper reservoir in spring. Walleyes and smallmouth bass were much less important predators on salmonids, and appeared to select subyearling chinook salmon only in August when the distribution of this prey overlapped with that of the predators. Size-selective predation by northern squawfish may also play an important role in reducing survival of the smaller individuals within each run of out-migrating juvenile salmonids.

  6. Incorporating spatial context into the analysis of salmonid habitat relations: Chapter 18

    USGS Publications Warehouse

    Torgersen, Christian E.; Baxter, Colden V.; Ebersole, J.L.; Gresswell, Bob; Church, Michael; Biron, Pascale M.; Roy, Andre G.

    2012-01-01

    In this response to the chapter by Lapointe (this volume), we discuss the question of why it is so difficult to predict salmonid-habitat relations in gravel-bed rivers and streams. We acknowledge that this cannot be an exhaustive treatment of the subject and, thus, identify what we believe are several key issues that demonstrate the necessity of incorporating spatial context into the analysis of fish-habitat data. Our emphasis is on spatial context (i.e., scale and location), but it is important to note that the same principles may be applied with some modification to temporal context, which is beyond the scope of this chapter.

  7. A new method to identify the fluvial regimes used by spawning salmonids

    Treesearch

    Hamish J. Moir; Christopher N. Gibbins; John M. Buffington; John H. Webb; Chris Soulsby; Mark J. Brewer

    2009-01-01

    Basin physiography and fluvial processes structure the availability of salmonid spawning habitat in river networks. However, methods that allow us to explicitly link hydrologic and geomorphic processes to spatial patterns of spawning at scales relevant to management are limited. Here we present a method that can be used to link the abundance of spawning salmonids to...

  8. Comparative Analysis of the Shared Sex-Determination Region (SDR) among Salmonid Fishes.

    PubMed

    Faber-Hammond, Joshua J; Phillips, Ruth B; Brown, Kim H

    2015-06-25

    Salmonids present an excellent model for studying evolution of young sex-chromosomes. Within the genus, Oncorhynchus, at least six independent sex-chromosome pairs have evolved, many unique to individual species. This variation results from the movement of the sex-determining gene, sdY, throughout the salmonid genome. While sdY is known to define sexual differentiation in salmonids, the mechanism of its movement throughout the genome has remained elusive due to high frequencies of repetitive elements, rDNA sequences, and transposons surrounding the sex-determining regions (SDR). Despite these difficulties, bacterial artificial chromosome (BAC) library clones from both rainbow trout and Atlantic salmon containing the sdY region have been reported. Here, we report the sequences for these BACs as well as the extended sequence for the known SDR in Chinook gained through genome walking methods. Comparative analysis allowed us to study the overlapping SDRs from three unique salmonid Y chromosomes to define the specific content, size, and variation present between the species. We found approximately 4.1 kb of orthologous sequence common to all three species, which contains the genetic content necessary for masculinization. The regions contain transposable elements that may be responsible for the translocations of the SDR throughout salmonid genomes and we examine potential mechanistic roles of each one. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  9. Persistence of Salmonid Redds

    NASA Astrophysics Data System (ADS)

    Buffington, J. M.; Buxton, T.; Fremier, A. K.; Hassan, M. A.; Yager, E.

    2013-12-01

    The construction of redds by spawning salmonids modifies fluvial processes in ways that are beneficial to egg and embryo survival. Redd topography induces hyporheic flow that oxygenates embryos incubating within the streambed and creates form drag that reduces bed mobility and scour of salmonid eggs. Winnowing of fine material during redd construction also coarsens the streambed, increasing bed porosity and hyporheic flow and reducing bed mobility. In addition to the biological benefits, redds may influence channel morphology by altering channel hydraulics and bed load transport rates depending on the size and extent of redds relative to the size of the channel. A key question is how long do the physical and biological effects of redds last? Field observations indicate that in some basins redds are ephemeral, with redd topography rapidly erased by subsequent floods, while in other basins, redds can persist for years. We hypothesize that redd persistence is a function of basin hydrology, sediment supply, and characteristics of the spawning fish. Hydrology controls the frequency and magnitude of bed mobilizing flows following spawning, while bed load supply (volume and caliber) controls the degree of textural fining and consequent bed mobility after spawning, as well as the potential for burial of redd features. The effectiveness of flows in terms of their magnitude and duration depend on hydroclimate (i.e., snowmelt, rainfall, or transitional hydrographs), while bed load supply depends on basin geology, land use, and natural disturbance regimes (e.g., wildfire). Location within the stream network may also influence redd persistence. In particular, lakes effectively trap sediment and regulate downstream flow, which may promote long-lived redds in stream reaches below lakes. These geomorphic controls are modulated by biological factors: fish species (size of fish controls size of redds and magnitude of streambed coarsening); life history (timing of spawning and

  10. Osmoregulatory actions of the GH/IGF axis in non-salmonid teleosts

    USGS Publications Warehouse

    Mancera, J.M.; McCormick, S.D.

    1998-01-01

    Salmonid fishes provided the first findings on the influence of the growth hormone (GH)/insulin-like growth factor I (IGF-I) axis on osmoregulation in teleost fishes. Recent studies on non-salmonid species, however, indicate that this physiological action of the GH/IGF-I axis is not restricted to salmonids or anadromous fishes. GH-producing cells in the pituitary of fish acclimated to different salinities show different degrees of activation depending on the species studied. Plasma GH levels either increase or do not change after transfer of fish from freshwater to seawater. Treatment with GH or IGF-I increases salinity tolerance and/or increases gill Na+,K+-ATPase activity of killifish (Fundulus heteroclitus), tilapia (Oreochromis mossambicus and Oreochromisniloticus) and striped bass (Morone saxatilis). As in salmonids, a positive interaction between GH and cortisol for improving hypoosmoregulatory capacity has been described in tilapia (O. mossambicus). Research on the osmoregulatory role of the GH/IGF-I axis is derived from a small number of teleost species. The study of more species with different osmoregulary patterns will be necessary to fully clarify the osmoregulatory role of GH/IGF-I axis in fish. The available data does suggest, however, that the influence of the GH/IGF-I axis on osmoregulation may be a common feature of euryhalinity in teleosts.

  11. Commencement Bay Study. Volume I. Summary and Synthesis.

    DTIC Science & Technology

    1981-12-31

    Volume II Land and Water Use Volume VI Physical Oceanography Volume III Fish and Wetlands Volume VII Sediments, Noise, Climate and Volume IV...Invertebrates Air Quality, Birds IS. KEY WORDS (Conthwe an fever"e *#do I 06ee87 end idenltf by block -her) Salmonids Wetlands Noise Aesthetics Marine Fish ...ENVIRONMENT 18 4 .1 GENERAL 18 4.2 BENTHIC INVERTSBRATUS 19 4.3 COMMERCIAL AND RECREATIONAL SHELLFISH 22 4.4 FISH 23 4.4.1 juvenile and Adult Salonids 24 4.4.2

  12. Ecosystem-based management of predator-prey relationships: piscivorous birds and salmonids.

    PubMed

    Wiese, Francis K; Parrish, Julia K; Thompson, Christopher W; Maranto, Christina

    2008-04-01

    Predator-prey relationships are often altered as a result of human activities. Where prey are legally protected, conservation action may include lethal predator control. In the Columbia River basin (Pacific Northwest, USA and Canada), piscivorous predators have been implicated in contributing to a lack of recovery of several endangered anadromous salmonids (Oncorhynchus spp.), and lethal and nonlethal control programs have been instituted against both piscine and avian species. To determine the consequences of avian predation, we used a bioenergetics approach to estimate the consumption of salmonid smolts by waterbirds (Common Merganser, California and Ring-billed Gull, Caspian Tern, Double-crested Cormorant) found in the mid-Columbia River from April through August, 2002-2004. We used our model to explore several predator-prey scenarios, including the impact of historical bird abundance, and the effect of preserving vs. removing birds, on smolt abundance. Each year, <1% of the estimated available salmonid smolts (interannual range: 44,830-109,209; 95% CI = 38,000-137,000) were consumed, 85-98% away from dams. Current diet data combined with historical gull abundance at dams suggests that past smolt consumption may have been 1.5-3 times current numbers, depending on the assumed distribution of gulls along the reaches. After the majority (80%) of salmonid smolts have left the study area, birds switch their diet to predominantly juvenile northern pikeminnow (Ptychocheilus oregonensis), which as adults are significant native salmonid predators in the Columbia River. Our models suggest that one consequence of removing birds from the system may be increased pikeminnow abundance, which--even assuming 80% compensatory mortality in juvenile pikeminnow survival--would theoretically result in an annual average savings of just over 180,000 smolts, calculated over a decade. Practically, this suggests that smolt survival could be maximized by deterring birds from the river when

  13. Feeding modes in stream salmonid population models: Is drift feeding the whole story?

    Treesearch

    Bret Harvey; Steve Railsback

    2014-01-01

    Drift-feeding models are essential components of broader models that link stream habitat to salmonid populations and community dynamics. But is an additional feeding mode needed for understanding and predicting salmonid population responses to streamflow and other environmental factors? We addressed this question by applying two versions of the individual-based model...

  14. Use of benthic prey by salmonids under turbid conditions in a laboratory stream

    Treesearch

    Bret C. Harvey; Jason L. White

    2008-01-01

    The negative effect of turbidity on the reactive distance of salmonids has been well established. However, determining the consequences of this relationship for overall feeding success remains problematic, as successful foraging by salmonids across a broad range in turbidity has been observed under a variety of conditions. Previous laboratory and field observations...

  15. Evaluating species-specific changes in hydrologic regimes: an iterative approach for salmonids in the Greater Yellowstone Area (USA)

    USGS Publications Warehouse

    Al-Chokhachy, Robert K.; Sepulveda, Adam; Ray, Andrew M.; Thoma, David P.; Tercek, Michael T.

    2017-01-01

    Despite the importance of hydrologic regimes to the phenology, demography, and abundance of fishes such as salmonids, there have been surprisingly few syntheses that holistically assess regional, species-specific trends in hydrologic regimes within a framework of climate change. Here, we consider hydrologic regimes within the Greater Yellowstone Area in the Rocky Mountains of western North America to evaluate changes in hydrologic metrics anticipated to affect salmonids, a group of fishes with high regional ecological and socioeconomic value. Our analyses assessed trends across different sites and time periods (1930–, 1950–, and 1970–2015) as means to evaluate spatial and temporal shifts. Consistent patterns emerged from our analyses indicating substantial shifts to (1) earlier peak discharge events; (2) reductions of summer minimum streamflows; (3) declines in the duration of river ice; and (4) decreases in total volume of water. We found accelerated trends in hydrologic change for the 1970–2015 period, with an average peak discharge 7.5 days earlier, 27.5% decline in summer minimum streamflows, and a 15.6% decline in the annual total volume of water (1 October–September 30) across sites. We did observe considerable variability in magnitude of change across sites, suggesting different levels of vulnerability to a changing climate. Our analyses provide an iterative means for assessing climate predictions and an important step in identifying the climate resilience of landscapes.

  16. Predation of juvenile salmonids by smallmouth bass and northern squawfish in the Columbia River near Richland, Washington

    USGS Publications Warehouse

    Tabor, Roger A.; Shively, Rip S.; Poe, Thomas P.

    1993-01-01

    The importance of juvenile salmonids in the diet of smallmouth bass Micropterus dolomieu and northern squawfish Ptychocheilus oregonensis was examined at a 6-km stretch of the Columbia River. Piscivorous fish were sampled with electrofishing gear on 4 d (May 2–3 and June 20–21, 1990) during emigration of juvenile anadromous salmonids. Sixty-two smallmouth bass and 69 northern squawfish were collected for diet analysis. Juvenile salmonids made up 59% of smallmouth bass diet by weight and were present in 65% of the stomachs of smallmouth bass. By a meal turnover method, smallmouth bass were estimated to consume from 1.4 (May 2–3) to 1.0 (June 20–21) salmonids per predator daily. Crayfish were the dominant prey item (41.4% by weight) of northern squawfish, but juvenile salmonids (28.8%) were also important. Northern squawfish consumed from 0.55 (May 2–3) to 0.34 (June 20–21) salmonids per predator daily. Smallmouth bass and northern squawfish consumed mostly subyearling Chinook salmon Oncorhynchus tshawytscha, which may have been wild Chinook salmon that emigrated downstream from the Hanford reach. Predation rates on salmonids by smallmouth bass are apparently high during spring and early summer because subyearling Chinook salmon are abundant and of suitable forage size and their habitat overlaps with that of smallmouth bass.

  17. Osmoregulatory actions of the GH/IGF axis in non-salmonid teleosts

    USGS Publications Warehouse

    Mancera, J.M.; McCormick, S.D.

    1998-01-01

    Salmonid fishes provided the first findings on the influence of the growth hormone (GH)/insulin-like growth factor I (IGF-I) axis on osmoregulation in teleost fishes. Recent studies on non-salmonid species, however, indicate that this physiological action of the GH/IGF-I axis is not restricted to salmonids or anadromous fishes. GH-producing cells in the pituitary of fish acclimated to different salinities show different degrees of activation depending on the species studied. Plasma GH levels either increase or do not change after transfer of fish from freshwater to seawater. Treatment with GH or IGF-I increases salinity tolerance and/or increases gill Na+,K+-ATPase activity of killifish (Fundulus heteroclitus), tilapia (Oreochromis mossambicus and Oreochromis niloticus) and striped bass (Morone saxatilis). As in salmonids, a positive interaction between GH and cortisol for improving hypoosmoregulatory capacity has been described in tilapia (O. mossambicus). Research on the osmoregulatory role of the GH/IGF-I axis is derived from a small number of teleost species. The study of more species with different osmoregulary patterns will be necessary to fully clarify the osmoregulatory role of GH/IGF-I axis in fish. The available data does suggest, however, that the influence of the GH/IGF-I axis on osmoregulation may be a common feature of euryhalinity in teleosts. Copyright (C) 1998 Elsevier Science Inc.

  18. Physiology and immunology of Lepeophtheirus salmonis infections of salmonids.

    PubMed

    Wagner, Glenn N; Fast, Mark D; Johnson, Stewart C

    2008-04-01

    'Sea lice' is a common name for a large number of species of marine ectoparasitic copepods, many of which are widespread and important disease-causing agents that infect both cultured and wild fish. Of these copepods, the salmon louse Lepeophtheirus salmonis is the most extensively studied because of its economic impact on the salmonid aquaculture industry and its possible impacts on wild salmonid populations. Different levels of infection by this parasite can affect the long-term survival and viability of its hosts. In this article, we review the nature of the interactions between L. salmonis and it hosts to identify crucial areas that warrant further research to aid understanding of the impact of infection with L. salmonis.

  19. Marine effect of introduced salmonids: Prey consumption by exotic steelhead and anadromous brown trout in the Patagonian Continental Shelf

    USGS Publications Warehouse

    Ciancio, J.; Beauchamp, D.A.; Pascual, M.

    2010-01-01

    On the basis of stable isotope analysis, we estimated the marine diet of the most abundant anadromous salmonid species in Patagonian Atlantic basins. The results were coupled with bioenergetic and population models to estimate the consumption of food by salmonids and was compared with that by seabirds, the most abundant top predators in the area. Amphipods were the main salmonid prey, followed by sprat, silversides, squid, and euphausiids. The total consumption, even assuming large anadromous salmonid populations, represented <5% of the total consumption by seabirds. We also identified the particular seabird colonies and artisanal fisheries with which salmonid trophic interactions at a more local scale could be significant. ?? 2010, by the American Society of Limnology and Oceanography, Inc.

  20. Is hyporheic flow an indicator for salmonid spawning site selection?

    NASA Astrophysics Data System (ADS)

    Benjankar, R. M.; Tonina, D.; Marzadri, A.; McKean, J. A.; Isaak, D.

    2015-12-01

    Several studies have investigated the role of hydraulic variables in the selection of spawning sites by salmonids. Some recent studies suggest that the intensity of the ambient hyporheic flow, that present without a salmon egg pocket, is a cue for spawning site selection, but others have argued against it. We tested this hypothesis by using a unique dataset of field surveyed spawning site locations and an unprecedented meter-scale resolution bathymetry of a 13.5 km long reach of Bear Valley Creek (Idaho, USA), an important Chinook salmon spawning stream. We used a two-dimensional surface water model to quantify stream hydraulics and a three-dimensional hyporheic model to quantify the hyporheic flows. Our results show that the intensity of ambient hyporheic flows is not a statistically significant variable for spawning site selection. Conversely, the intensity of the water surface curvature and the habitat quality, quantified as a function of stream hydraulics and morphology, are the most important variables for salmonid spawning site selection. KEY WORDS: Salmonid spawning habitat, pool-riffle system, habitat quality, surface water curvature, hyporheic flow

  1. Model structure of the stream salmonid simulator (S3)—A dynamic model for simulating growth, movement, and survival of juvenile salmonids

    USGS Publications Warehouse

    Perry, Russell W.; Plumb, John M.; Jones, Edward C.; Som, Nicholas A.; Hetrick, Nicholas J.; Hardy, Thomas B.

    2018-04-06

    Fisheries and water managers often use population models to aid in understanding the effect of alternative water management or restoration actions on anadromous fish populations. We developed the Stream Salmonid Simulator (S3) to help resource managers evaluate the effect of management alternatives on juvenile salmonid populations. S3 is a deterministic stage-structured population model that tracks daily growth, movement, and survival of juvenile salmon. A key theme of the model is that river flow affects habitat availability and capacity, which in turn drives density dependent population dynamics. To explicitly link population dynamics to habitat quality and quantity, the river environment is constructed as a one-dimensional series of linked habitat units, each of which has an associated daily time series of discharge, water temperature, and usable habitat area or carrying capacity. The physical characteristics of each habitat unit and the number of fish occupying each unit, in turn, drive survival and growth within each habitat unit and movement of fish among habitat units.The purpose of this report is to outline the underlying general structure of the S3 model that is common among different applications of the model. We have developed applications of the S3 model for juvenile fall Chinook salmon (Oncorhynchus tshawytscha) in the lower Klamath River. Thus, this report is a companion to current application of the S3 model to the Trinity River (in review). The general S3 model structure provides a biological and physical framework for the salmonid freshwater life cycle. This framework captures important demographics of juvenile salmonids aimed at translating management alternatives into simulated population responses. Although the S3 model is built on this common framework, the model has been constructed to allow much flexibility in application of the model to specific river systems. The ability for practitioners to include system-specific information for the

  2. Smallmouth bass and largemouth bass predation on juvenile Chinook salmon and other salmonids in the Lake Washington basin

    USGS Publications Warehouse

    Tabor, R.A.; Footen, B.A.; Fresh, K.L.; Celedonia, M.T.; Mejia, F.; Low, D.L.; Park, L.

    2007-01-01

    We assessed the impact of predation by smallmouth bass Micropterus dolomieu and largemouth bass M. salmoides on juveniles of federally listed Chinook salmon Oncorhynchus tshawytscha and other anadromous salmonid populations in the Lake Washington system. Bass were collected with boat electrofishing equipment in the south end of Lake Washington (February-June) and the Lake Washington Ship Canal (LWSC; April-July), a narrow waterway that smolts must migrate through to reach the marine environment. Genetic analysis was used to identify ingested salmonids to obtain a more precise species-specific consumption estimate. Overall, we examined the stomachs of 783 smallmouth bass and 310 largemouth bass greater than 100 mm fork length (FL). Rates of predation on salmonids in the south end of Lake Washington were generally low for both black bass species. In the LWSC, juvenile salmonids made up a substantial part of bass diets; consumption of salmonids was lower for largemouth bass than for smallmouth bass. Smallmouth bass predation on juvenile salmonids was greatest in June, when salmonids made up approximately 50% of their diet. In the LWSC, overall black bass consumption of salmonids was approximately 36,000 (bioenergetics model) to 46,000 (meal turnover consumption model) juveniles, of which about one-third was juvenile Chinook salmon, one-third was coho salmon O. kisutch, and one-third was sockeye salmon O. nerka. We estimated that about 2,460,000 juvenile Chinook salmon (hatchery and wild sources combined) were produced in the Lake Washington basin in 1999; thus, the mortality estimates in the LWSC range from 0.5% (bioenergetics) to 0.6% (meal turnover). Black bass prey mostly on subyearlings of each salmonid species. The vulnerability of subyearlings to predation can be attributed to their relatively small size; their tendency to migrate when water temperatures exceed 15??C, coinciding with greater black bass activity; and their use of nearshore areas, where overlap

  3. Migratory salmonid redd habitat characteristics in the Salmon River, New York

    USGS Publications Warehouse

    Johnson, James H.; Nack, Christopher C.; McKenna, James E.

    2010-01-01

    Non-native migratory salmonids ascend tributaries to spawn in all the Great Lakes. In Lake Ontario, these species include Chinook salmon (Oncorhynchus tshawytscha), coho salmon (O. kisutch), steelhead (O. mykiss), and brown trout (Salmo trutta). Although successful natural reproduction has been documented for many of these species, little research has been conducted on their spawning habitat. We examined the spawning habitat of these four species in the Salmon River, New York. Differences in fish size among the species were significantly correlated with spawning site selection. In the Salmon River, the larger species spawned in deeper areas with larger size substrate and made the largest redds. Discriminant function analysis correctly classified redds by species 64–100% of the time. The size of substrate materials below Lighthouse Hill Dam is within the preferred ranges for spawning for these four species indicating that river armoring has not negatively impacted salmonid production. Intra-specific and inter-specific competition for spawning sites may influence redd site selection for smaller salmonids and could be an impediment for Atlantic salmon (S. salar) restoration.

  4. The footprint of salmonids on river morphology

    NASA Astrophysics Data System (ADS)

    Hassan, M. A.; Tonina, D.

    2012-12-01

    Female salmonids dig a pit in the streambed where they lay their eggs, which then cover with sediment from a second pit forming an egg nest call redd. This formation results in a shape resembling a dune with an amplitude, which is the vertical difference between bottom of the pit and crest of the hump, varying from few centimetres (for small fish, chum or sockeye salmon) to tenths of a meter (for large fish, Chinook salmon). During redd construction, salmonids alter streambed topography, winnow away fine sediment and mix streambed material within a layer as thick as 50 cm, for the large chinook salmon. The spawning activities may result in additional roughness at the local scale due to redds. However, redd construction may smooth large-scale topography reducing roughness due the macro-bedform. These topographical changes vary streambed roughness, which in turn may affect shear stress distribution. Redds have been suggested to increase the overall flow resistance due to form drag resulting in lower grain shear stress and less particle mobility. However, the mixing of the sediment could prevent armouring of the streambed surface allowing higher than with armouring sediment transport. Here, we use detailed pre- and post-spawning bathymetries coupled with accurate 2-dimensional hydraulic numerical modelling to test which of these two effects has potentially more impact on sediment transport. Our results show that topographical roughness added by sockeye salmons, which build small redds with 15cm amplitude and 1 meter wavelength (longitudinal length of a redd), has negligible effect on shear stress at the reach-scale and limited at the local scale. Conversely, sediment mixing has an important effect on reducing armouring, increasing sediment mobility, which results in potentially more sediment transport in reaches with than without redds. Consequently, salmonid bioturbation due to mass-spawning fish can be a dominant element for sediment transport in mountain drainage

  5. Low summer water temperatures influence occurrence of naturalized salmonids across a mountain watershed

    USGS Publications Warehouse

    Mullner, S.A.; Hubert, W.A.

    2005-01-01

    We investigated relationships between the absence of salmonids and low summer water temperatures across a 150-km2 Rocky Mountain watershed. A model predicting maximum July water temperature (MJT) from measurements of perennial stream length, wetted width, and midrange basin elevation was developed from temperature data obtained at 20 sites across the watershed. The model was used to predict MJT in 75 reaches across the watershed where salmonids were sampled. The lowest predicted MJT in reaches where age-0 and juvenile-adult brook trout Salvelinus fontinalis were observed was 9??C. The lowest predicted MJT in reaches where age-0 progeny of the genus Oncorhynchus spp. (i.e., rainbow trout O. mykiss or cutthroat trout O. clarkii) were observed was 13??C and where Oncorhynchus spp. adults where observed was 12??C. The probability of occurrence of both age-0 and adult brook trout and Oncorhynchus spp. increased as MJT increased above these thresholds. Our results indicate that low MJT in some portions of a mountain watershed can be related to the absence of salmonids. Consequently, data on MJT may provide managers with a means of assessing where summer water temperatures are not suitable for establishment of naturalized salmonid populations. ?? Copyright by the American Fisheries Society 2005.

  6. Modeling effects of climate change on Yakima River salmonid habitats

    USGS Publications Warehouse

    Hatten, James R.; Batt, Thomas R.; Connolly, Patrick J.; Maule, Alec G.

    2014-01-01

    We evaluated the potential effects of two climate change scenarios on salmonid habitats in the Yakima River by linking the outputs from a watershed model, a river operations model, a two-dimensional (2D) hydrodynamic model, and a geographic information system (GIS). The watershed model produced a discharge time series (hydrograph) in two study reaches under three climate scenarios: a baseline (1981–2005), a 1-°C increase in mean air temperature (plus one scenario), and a 2-°C increase (plus two scenario). A river operations model modified the discharge time series with Yakima River operational rules, a 2D model provided spatially explicit depth and velocity grids for two floodplain reaches, while an expert panel provided habitat criteria for four life stages of coho and fall Chinook salmon. We generated discharge-habitat functions for each salmonid life stage (e.g., spawning, rearing) in main stem and side channels, and habitat time series for baseline, plus one (P1) and plus two (P2) scenarios. The spatial and temporal patterns in salmonid habitats differed by reach, life stage, and climate scenario. Seventy-five percent of the 28 discharge-habitat responses exhibited a decrease in habitat quantity, with the P2 scenario producing the largest changes, followed by P1. Fry and spring/summer rearing habitats were the most sensitive to warming and flow modification for both species. Side channels generally produced more habitat than main stem and were more responsive to flow changes, demonstrating the importance of lateral connectivity in the floodplain. A discharge-habitat sensitivity analysis revealed that proactive management of regulated surface waters (i.e., increasing or decreasing flows) might lessen the impacts of climate change on salmonid habitats.

  7. Predation by Resident Fish on Juvenile Salmonids in John Day Reservoir: Final Report, 1983-1986: Volume 1, Final Report of Research.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Poe, Thomas P.; Rieman, Bruce E.

    1988-07-01

    In 1982 the NPPC included in its Fish and Wildlife Program a measure that called for studies ''... to investigate juvenile salmon and steelhead losses to predators while these fish are migrating through Columbia and Snake River reservoirs.'' In the same year the Bonneville Power Administration (BPA) funded ODFW and FWS to conduct collaborative studies to estimate the number of juvenile salmonids lost to predators in John Day Reservoir. Also included as study objectives were: (1) a description of the importance of predation losses relative to mortality at the dam and total reservoir mortality; (2) a description of how predationmore » losses might vary (spatially and temporally); and (3) recommendations of measures to control predation on smolts. We studied four species of predator: northern squawfish, walleye, smallmouth bass, and channel catfish. We selected John Day Reservoir as the study site because the following factors led us to believe if predation was a problem in any reservoir, it would be most obvious there because: (1) the reservoir is an important subyearling chinook rearing area; (2) passage and residualism of juvenile salmonids were considered a problem there; and (3) substantial populations of predators were known to reside in the reservoir. Individual reports were processed separately for the data base.« less

  8. Juvenile and resident salmonid movement and passage through culverts

    DOT National Transportation Integrated Search

    1998-07-01

    An outcome of the Washington State Department of Transportation's Juvenile Fish Passage Workshop on September 24, 1997, was agreement that a literature review was necessary to determine the state of knowledge about juvenile salmonid movement and pass...

  9. [Operation and interaction peculiarities of diagnostic laboratories involved in providing protection from infectious diseases during the XXII Olympic Winter Games and XI Paralympic Winter Games 2014 in Sochi].

    PubMed

    Onishenko, G G; Popova, A Iu; Bragina, I V; Kuz'kin, B P; Ezhlova, E B; Demina, Iu V; Gus'kov, A S; Ivanov, G E; Chikina, L V; Klindukhova, V P; Grechanaia, T V; Tesheva, S Ch; Kulichenko, A N; Efremenko, D B; Manin, E A; Kuznetsova, I V; Parkhomenko, V V; Kulichenko, O A; Rafeenko, G K; Shcherbina, L I; Zavora, D L; Briukhanov, A F; Eldinova, V E; Iunicheva, Iu V; Derliatko, S K; Komarov, N S

    2015-01-01

    The experience of the organization and functioning of the laboratory network during the XXII Olympic Winter Games and XI Paralympic Winter Games of 2014 in Sochi is considered. Efforts to establish an effective system of laboratory support, the order of work and interaction of diagnostic laboratories involved in diseases control of population during the Olympic Games are analyzed.

  10. Genetics and genomics of disease resistance in salmonid species

    PubMed Central

    Yáñez, José M.; Houston, Ross D.; Newman, Scott

    2014-01-01

    Infectious and parasitic diseases generate large economic losses in salmon farming. A feasible and sustainable alternative to prevent disease outbreaks may be represented by genetic improvement for disease resistance. To include disease resistance into the breeding goal, prior knowledge of the levels of genetic variation for these traits is required. Furthermore, the information from the genetic architecture and molecular factors involved in resistance against diseases may be used to accelerate the genetic progress for these traits. In this regard, marker assisted selection and genomic selection are approaches which incorporate molecular information to increase the accuracy when predicting the genetic merit of selection candidates. In this article we review and discuss key aspects related to disease resistance in salmonid species, from both a genetic and genomic perspective, with emphasis in the applicability of disease resistance traits into breeding programs in salmonids. PMID:25505486

  11. Variation in salmonid life histories: patterns and perspectives.

    Treesearch

    Mary F. Willson

    1997-01-01

    Salmonid fishes differ in degree of anadromy, age of maturation, frequency of reproduction, body size and fecundity, sexual dimorphism, breeding season, morphology, and, to a lesser degree, parental care. Patterns of variation and their possible significance for ecology and evolution and for resource management are the focus of this review.

  12. Sublethal toxicity of chlorpyrifos to salmonid olfaction after hypersaline acclimation.

    PubMed

    Maryoung, Lindley A; Blunt, Brian; Tierney, Keith B; Schlenk, Daniel

    2015-04-01

    Salmonid habitats can be impacted by several environmental factors, such as salinization, which can also affect salmonid tolerance to anthropogenic stressors, such as pesticides. Previous studies have shown that hypersaline acclimation enhances the acute toxicity of certain organophosphate and carbamate pesticides to euryhaline fish; however, sublethal impacts have been far less studied. The current study aims to determine how hypersaline acclimation and exposure to the organophosphate chlorpyrifos (CPF) impact salmonid olfaction. Combined acclimation and exposure to CPF was shown to impact rainbow trout olfaction at the molecular, physiological, and behavioral levels. Concurrent exposure to hypersalinity and 0.5μg/L CPF upregulated four genes (chloride intracellular channel 4, G protein zgc:101761, calcium calmodulin dependent protein kinase II delta, and adrenergic alpha 2C receptor) that inhibit olfactory signal transduction. At the physiological level, hypersalinity and chlorpyrifos caused a decrease in sensory response to the amino acid l-serine and the bile salt taurocholic acid. Combined acclimation and exposure also negatively impacted behavior and reduced the avoidance of a predator cue (l-serine). Thus, acclimation to hypersaline conditions and exposure to environmentally relevant concentrations of chlorpyrifos caused an inhibition of olfactory signal transduction leading to a decreased response to odorants and impairment of olfactory mediated behaviors. Copyright © 2015 Elsevier B.V. All rights reserved.

  13. Effects of Hatchery Rearing on the Structure and Function of Salmonid Mechanosensory Systems.

    PubMed

    Brown, Andrew D; Sisneros, Joseph A; Jurasin, Tyler; Coffin, Allison B

    2016-01-01

    This paper reviews recent studies on the effects of hatchery rearing on the auditory and lateral line systems of salmonid fishes. Major conclusions are that (1) hatchery-reared juveniles exhibit abnormal lateral line morphology (relative to wild-origin conspecifics), suggesting that the hatchery environment affects lateral line structure, perhaps due to differences in the hydrodynamic conditions of hatcheries versus natural rearing environments, and (2) hatchery-reared salmonids have a high proportion of abnormal otoliths, a condition associated with reduced auditory sensitivity and suggestive of inner ear dysfunction.

  14. Feeding response by northern squawfish to a hatchery release of juvenile salmonids in the Clearwater River, Idaho

    USGS Publications Warehouse

    Shively, R.S.; Poe, T.P.; Sauter, S.T.

    1996-01-01

    We collected gut contents from northern squawfish Ptychocheilus oregonensis captured in the Clearwater River, Idaho, 0–6 km from its confluence with the Snake River, following the release of 1.1 million yearling chinook salmon Oncorhynchus tshawytscha from the Dworshak National Fish Hatchery. Before the hatchery release, northern squawfish gut contents (by weight) in the study area were 38% crayfish Pacifastacus spp., 26% insects, 19% nonsalmonid fish, and 16% wheat kernels Triticum spp. Juvenile salmonids constituted 54% of gut contents about 24 h after the hatchery release, 78% after 5 d, and 86% after 7 d. The mean number of salmonids per gut (1.2) after release was higher than typically seen in guts from northern squawfish collected in mid-reservoir areas away from hydroelectric dams on the Snake and Columbia rivers. Length-frequency distributions of juvenile salmonids eaten and those captured in a scoop trap 4 km upstream of the study area indicated that northern squawfish were selectively feeding on the smaller individuals. We attribute the high rates of predation in the study area to the artificially high density of juvenile salmonids resulting from the hatchery release and to the physical characteristics of the study area in which the river changed from free flowing to impounded. Our results suggest that northern squawfish can quickly exploit hatchery releases of juvenile salmonids away from release sites in the Columbia River basin.

  15. Genetic variation among Flavobacterium psychrophilum isolates from wild and farmed salmonids in Norway and Chile.

    PubMed

    Apablaza, P; Løland, A D; Brevik, Ø J; Ilardi, P; Battaglia, J; Nylund, A

    2013-04-01

    To aim of the study was to describe the genetic relationship between isolates of Flavobacterium psychrophilum with a main emphasis of samples from Chile and Norway. The isolates have been obtained from farmed salmonids in Norway and Chile, and from wild salmonids in Norway, but isolates from North America and European countries are also included in the analysis. The study is based on phylogenetic analysis of 16S rRNA and seven housekeeping genes (HG), gyrB, atpA, dnaK, trpB, fumC, murG and tuf, and the use of a multilocus sequence typing (MLST) system, based on nucleotide polymorphism in the HG, as an alternative to the phylogenies. The variation within the selected genes was limited, and the phylogenetic analysis gave little resolution between the isolates. The MLST gave a much better resolution resulting in 53 sequence types where the same sequences types could be found in Chile, North America and European countries, and in different host species. Multilocus sequence typing give a relatively good separation of different isolates of Fl. psychrophilum and show that there are no distinct geographical or host-specific isolates in the studied material from Chile, North America and Europe. Nor was it possible to separate between isolates from ulcers and systemic infections vs isolates from the surface of healthy salmonids. This study shows a wide geographical distribution of Fl. psychrophilum, indicating that the bacterium has a large potential for transmission over long distances, and between different salmonid hosts species. This knowledge will be important for future management of salmonids diseases connected to Fl. psychrophilum. © 2013 The Society for Applied Microbiology.

  16. System-Wide Significance of Predation on Juvenile Salmonids in Columbia and Snake River Reservoirs : Annual Report of Research 1990.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Petersen, C.J.

    1991-03-01

    The consumption rates of northern squawfish (Ptychocheilus oregonensis) preying upon juvenile salmonids were indexed in four reservoirs (Bonneville, The Dalles, John Day and McNary) of the lower Columbia River. During the spring and summer of 1990, over 2000 northern squawfish were collected from dam forebays, dam tailraces and mid-reservoir locations. Gut content data, predator weight and water temperature were used to compute a consumption index (CI) for northern squawfish. Juvenile salmonids were found in 435 of 1598 northern squawfish guts analyzed. Besides salmonids and other preyfish, crustaceans formed a significant portion of the diet. The CI of northern squawfish variedmore » by season and location. At most locations, summer CI's of northern squawfish were higher than in the spring. Efforts to match sample collection with times of highest juvenile salmonid passage were successful except during July at The Dalles and Bonneville Reservoirs. Consumption indices were moderate to high at several locations even when passage was relatively low, suggesting salmonid predation rate by northern squawfish was not always a function of prey density. 19 refs., 5 figs., 12 tabs.« less

  17. Underwater methods for study of salmonids in the Intermountain West

    Treesearch

    Russell F. Thurow

    1994-01-01

    This guide describes underwater methods using snorkeling gear to study fish populations in flowing waters of the Intermountain West. It outlines procedures for estimating salmonid abundance and habitat use and provides criteria for identifying and estimating the size of fish underwater.

  18. Modeling potential river management conflicts between frogs and salmonids

    Treesearch

    Steven F. Railsback; Bret C. Harvey; Sarah J. Kupferberg; Margaret M. Lang; Scott McBain; Hart H. Welsh

    2016-01-01

    Management of regulated rivers for yellow-legged frogs (Rana boylii) and salmonids exemplifies potential conflicts among species adapted to different parts of the natural flow and temperature regimes. Yellow-legged frogs oviposit in rivers in spring and depend on declining flows and warming temperatures for egg and tadpole survival and growth,...

  19. Developments in the control of bacterial kidney disease of salmonid fishes

    USGS Publications Warehouse

    Elliott, D.G.; Pascho, R.J.; Bullock, G.L.

    1989-01-01

    Bacterial kidney disease of salmonid fishes, caused by Renibactenum salrnoninarum, was first reported more than 50 yr ago; nevertheless, large gaps persist in our knowledge of the infection - particularly in methods for its control. In the 1950's, principal control measures consisted of prophylactic or therapeutic feeding of sulfonamides, which were later supplanted by the antibiotic erythromycin. Chemotherapy has effected some reduction of mortality, but benefits are typically transient and mortality usually resumes after the drug is withdrawn. Some studies have indicated that diet composition affects the prevalence and severity of the disease. Although tests of chemotherapeutants and diet modification have continued, research emphasis has shifted partly toward prevention of the disease by breaking the infection cycle. It is now generally accepted that R. salrnoninarum can be transmitted both vertically and horizontally. Experimental evidence indicates that immersion of newly fertilized eggs in iodophor or erythromycin does not prevent vertical transmission. However, the injection of female salmon with erythromycin before they spawn shows promise as a practical means of interrupting vertical transmission. The results of attempts to prevent infection of juvenile salmonids by vaccination against bacterial kidney disease have been disappointing, thus underscoring a basic need for a better understanding of protective mechanisms in salmonids. The recent development of more sensitive and quantitative detection methods should aid in evaluating the efficacy of current and future control strategies.

  20. An efficient and reliable DNA-based sex identification method for archaeological Pacific salmonid (Oncorhynchus spp.) remains.

    PubMed

    Royle, Thomas C A; Sakhrani, Dionne; Speller, Camilla F; Butler, Virginia L; Devlin, Robert H; Cannon, Aubrey; Yang, Dongya Y

    2018-01-01

    Pacific salmonid (Oncorhynchus spp.) remains are routinely recovered from archaeological sites in northwestern North America but typically lack sexually dimorphic features, precluding the sex identification of these remains through morphological approaches. Consequently, little is known about the deep history of the sex-selective salmonid fishing strategies practiced by some of the region's Indigenous peoples. Here, we present a DNA-based method for the sex identification of archaeological Pacific salmonid remains that integrates two PCR assays that each co-amplify fragments of the sexually dimorphic on the Y chromosome (sdY) gene and an internal positive control (Clock1a or D-loop). The first assay co-amplifies a 95 bp fragment of sdY and a 108 bp fragment of the autosomal Clock1a gene, whereas the second assay co-amplifies the same sdY fragment and a 249 bp fragment of the mitochondrial D-loop region. This method's reliability, sensitivity, and efficiency, were evaluated by applying it to 72 modern Pacific salmonids from five species and 75 archaeological remains from six Pacific salmonids. The sex identities assigned to each of the modern samples were concordant with their known phenotypic sex, highlighting the method's reliability. Applications of the method to dilutions of modern DNA samples indicate it can correctly identify the sex of samples with as little as ~39 pg of total genomic DNA. The successful sex identification of 70 of the 75 (93%) archaeological samples further demonstrates the method's sensitivity. The method's reliance on two co-amplifications that preferentially amplify sdY helps validate the sex identities assigned to samples and reduce erroneous identifications caused by allelic dropout and contamination. Furthermore, by sequencing the D-loop fragment used as a positive control, species-level and sex identifications can be simultaneously assigned to samples. Overall, our results indicate the DNA-based method reported in this study is a

  1. River Temperature Dynamics and Habitat Characteristics as Predictors of Salmonid Abundance using Fiber-Optic Distributed Temperature Sensing

    NASA Astrophysics Data System (ADS)

    Gryczkowski, L.; Gallion, D.; Haeseker, S.; Bower, R.; Collier, M.; Selker, J. S.; Scherberg, J.; Henry, R.

    2011-12-01

    Salmonids require cool water for all life stages, including spawning and growth. Excessive water temperature causes reduced growth and increased disease and mortality. During the summer, salmonids seek local zones of cooler water as a refuge from elevated temperatures. They also prefer specific habitat features such as boulders and overhanging vegetation. The purpose of this study is to determine whether temperature dynamics or commonly measured fish habitat metrics best explain salmonid abundance. The study site was a 2-kilometer reach of the Walla Walla River near Milton-Freewater, OR, USA, which provides habitat for the salmonids chinook salmon (Oncorhynchus tshawytscha), steelhead/rainbow trout (Oncorhynchus mykiss), mountain whitefish (Prosopium williamsoni), and the endangered bull trout (Salvelinus confluentus). The Walla Walla River is listed as an impaired water body under section 303(d) of the Clean Water Act due to temperature. The associated total maximum daily load (TMDL) calls for temperatures to be below 18 °C at all times for salmonid rearing and migration; however, river temperatures surpassed 24 °C in parts of the study reach in 2009. The two largest factors contributing to the warmer water are reduced riparian vegetation, which decreases shading and increases direct solar radiation, and decreased summer flows caused by diversions and irrigation for agriculture. Fiber-optic distributed temperature sensing has emerged as a unique and powerful tool for ecological applications because of its high spatial and temporal resolution. In this study, meter-scale temperature measurements were obtained at 15-minute intervals along the length of the study reach, allowing for the detection and quantification of cold water inflows during the summer of 2009. The cold water inflows were classified as groundwater or hyporheic sources based on the diurnal temperature patterns. Snorkel surveys were conducted in mid-July and mid-August, 2009 to enumerate salmonid

  2. The energetic consequences of habitat structure for forest stream salmonids.

    PubMed

    Naman, Sean M; Rosenfeld, Jordan S; Kiffney, Peter M; Richardson, John S

    2018-05-08

    1.Increasing habitat availability (i.e. habitat suitable for occupancy) is often assumed to elevate the abundance or production of mobile consumers; however, this relationship is often nonlinear (threshold or unimodal). Identifying the mechanisms underlying these nonlinearities is essential for predicting the ecological impacts of habitat change, yet the functional forms and ultimate causation of consumer-habitat relationships are often poorly understood. 2.Nonlinear effects of habitat on animal abundance may manifest through physical constraints on foraging that restrict consumers from accessing their resources. Subsequent spatial incongruence between consumers and resources should lead to unimodal or saturating effects of habitat availability on consumer production if increasing the area of habitat suitable for consumer occupancy comes at the expense of habitats that generate resources. However, the shape of this relationship could be sensitive to cross-ecosystem prey subsidies, which may be unrelated to recipient habitat structure and result in more linear habitat effects on consumer production. 3.We investigated habitat-productivity relationships for juveniles of stream-rearing Pacific salmon and trout (Oncorhynchus spp.), which typically forage in low-velocity pool habitats, while their prey (drifting benthic invertebrates) are produced upstream in high-velocity riffles. However, juvenile salmonids also consume subsidies of terrestrial invertebrates that may be independent of pool-riffle structure. 4.We measured salmonid biomass production in 13 experimental enclosures each containing a downstream pool and upstream riffle, spanning a gradient of relative pool area (14-80% pool). Increasing pool relative to riffle habitat area decreased prey abundance, leading to a nonlinear saturating effect on fish production. We then used bioenergetics model simulations to examine how the relationship between pool area and salmonid biomass is affected by varying levels of

  3. Fish and chips: Various methodologies demonstrate utility of a 16,006-gene salmonid microarray

    PubMed Central

    von Schalburg, Kristian R; Rise, Matthew L; Cooper, Glenn A; Brown, Gordon D; Gibbs, A Ross; Nelson, Colleen C; Davidson, William S; Koop, Ben F

    2005-01-01

    Background We have developed and fabricated a salmonid microarray containing cDNAs representing 16,006 genes. The genes spotted on the array have been stringently selected from Atlantic salmon and rainbow trout expressed sequence tag (EST) databases. The EST databases presently contain over 300,000 sequences from over 175 salmonid cDNA libraries derived from a wide variety of tissues and different developmental stages. In order to evaluate the utility of the microarray, a number of hybridization techniques and screening methods have been developed and tested. Results We have analyzed and evaluated the utility of a microarray containing 16,006 (16K) salmonid cDNAs in a variety of potential experimental settings. We quantified the amount of transcriptome binding that occurred in cross-species, organ complexity and intraspecific variation hybridization studies. We also developed a methodology to rapidly identify and confirm the contents of a bacterial artificial chromosome (BAC) library containing Atlantic salmon genomic DNA. Conclusion We validate and demonstrate the usefulness of the 16K microarray over a wide range of teleosts, even for transcriptome targets from species distantly related to salmonids. We show the potential of the use of the microarray in a variety of experimental settings through hybridization studies that examine the binding of targets derived from different organs and tissues. Intraspecific variation in transcriptome expression is evaluated and discussed. Finally, BAC hybridizations are demonstrated as a rapid and accurate means to identify gene content. PMID:16164747

  4. Prevention and control of viral diseases of salmonids

    USGS Publications Warehouse

    Amend, Donald F.

    1976-01-01

    Three viral diseases of salmonids are of worldwide concern: infectious pancreatic necrosis (IPN) viral hemorrhagic septicemia (VHS), and infectious hematopoietic necrosis (IHN). Six principal approaches are being used to prevent or control these diseases: 1) preventing contact o the pathogen with the host, 2) environmental manipulation, 3) immunization, 4) chemotherapy, 5 selective breeding for disease resistance, and 6) reducing stress conditions which augment disease conditions. Preventing the introduction of a pathogen into a new stock of fish has been accomplished mainly by implementing stringent laws to prevent transport of infected fish into uninfected areas. Stocks of fish already infected are sometimes destroyed, and the hatchery is disinfected and restocked with fish free of specific pathogens. Environmental manipulation (elevated water temperature) has been successfully used to control IHN. Chemotherapeutics such as povidone-iodine for IPN and benzipyrene for IHN show promise of controlling mortalities; however, the practicality of using these drugs to eliminate the carrier fish has not been evaluated. Salmonids are capable of developing immune responses to viruses; however, development of effective vaccines, selective breeding for disease resistance, and identification of stress conditions which augment disease are still in the experimental phase.

  5. Predicting recolonization patterns and interactions between potamodromous and anadromous salmonids in response to dam removal in the Elwha River, Washington State, USA

    USGS Publications Warehouse

    Brenkman, S.J.; Pess, G.R.; Torgersen, C.E.; Kloehn, K.K.; Duda, J.J.; Corbett, S.C.

    2008-01-01

    The restoration of salmonids in the Elwha River following dam removal will cause interactions between anadromous and potamodromous forms as recolonization occurs in upstream and downstream directions. Anadromous salmonids are expected to recolonize historic habitats, and rainbow trout (Oncorhynchus mykiss) and bull trout (Salvelinus confluentus) isolated above the dams for 90 years are expected to reestablish anadromy. We summarized the distribution and abundance of potamodromous salmonids, determined locations of spawning areas, and mapped natural barriers to fish migration at the watershed scale based on data collected from 1993 to 2006. Rainbow trout were far more abundant than bull trout throughout the watershed and both species were distributed up to river km 71. Spawning locations for bull trout and rainbow trout occurred in areas where we anticipate returning anadromous fish to spawn. Nonnative brook trout were confined to areas between and below the dams, and seasonal velocity barriers are expected to prevent their upstream movements. We hypothesize that the extent of interaction between potamodromous and anadromous salmonids will vary spatially due to natural barriers that will limit upstream-directed recolonization for some species of salmonids. Consequently, most competitive interactions will occur in the main stem and floodplain downstream of river km 25 and in larger tributaries. Understanding future responses of Pacific salmonids after dam removal in the Elwha River depends upon an understanding of existing conditions of the salmonid community upstream of the dams prior to dam removal.

  6. Estimated loss of juvenile salmonids to predation by northern squawfish, walleyes, and smallmouth bass in John Day Reservoir, Columbia River

    USGS Publications Warehouse

    Rieman, Bruce E.; Beamesderfer, Raymond C.; Vigg, Steven; Poe, Thomas P.

    1991-01-01

    We estimated the loss of juvenile salmonids Oncorhynchus spp. to predation by northern squawfish Ptychocheilus oregonensis, walleyes Stizostedion vitreum, and smallmouth bass Micropterus dolomieu in John Day Reservoir during 1983–1986. Our estimates were based on measures of daily prey consumption, predator numbers, and numbers of juvenile salmonids entering the reservoir during the April–August period of migration. We estimated the mean annual loss was 2.7 million juvenile salmonids (95% confidence interval, 1.9–3.3 million). Northern squawfish were responsible for 78% of the total loss; walleyes accounted for 13% and smallmouth bass for 9%. Twenty-one percent of the loss occurred in a small area immediately below McNary Dam at the head of John Day Reservoir. We estimated that the three predator species consumed 14% (95% confidence interval, 9–19%) of all juvenile salmonids that entered the reservoir. Mortality changed by month and increased late in the migration season. Monthly mortality estimates ranged from 7% in June to 61% in August. Mortality from predation was highest for chinook salmon O. tshawytscha, which migrated in July and August. Despite uncertainties in the estimates, it is clear that predation by resident fish predators can easily account for previously unexplained mortality of out-migrating juvenile salmonids. Alteration of the Columbia River by dams and a decline in the number of salmonids could have increased the fraction of mortality caused by predation over what it was in the past.

  7. A salmonid EST genomic study: genes, duplications, phylogeny and microarrays

    USDA-ARS?s Scientific Manuscript database

    Background: Salmonids are of interest because of their relatively recent genome duplication, and their extensive use in wild fisheries and aquaculture. A comprehensive gene list and a comparison of genes in some of the different species provide valuable genomic information for one of the most wide...

  8. Strategies for conserving native salmonid populations at risk from nonnative fish invasions: tradeoffs in using barriers to upstream movement

    Treesearch

    Kurt D. Fausch; Bruce E. Rieman; Michael Young; Jason B. Dunham

    2006-01-01

    Native salmonid populations in the inland West are often restricted to small isolated habitats at risk from invasion by nonnative salmonids. However, further isolating these populations using barriers to prevent invasions can increase their extinction risk. This monograph reviews the state of knowledge about this tradeoff between invasion and isolation. We present a...

  9. Piscine reovirus in wild and farmed salmonids in British Columbia, Canada: 1974-2013.

    PubMed

    Marty, G D; Morrison, D B; Bidulka, J; Joseph, T; Siah, A

    2015-08-01

    Piscine reovirus (PRV) was common among wild and farmed salmonids in British Columbia, western Canada, from 1987 to 2013. Salmonid tissues tested for PRV by real-time rRT-PCR included sections from archived paraffin blocks from 1974 to 2008 (n = 363) and fresh-frozen hearts from 2013 (n = 916). The earliest PRV-positive sample was from a wild-source steelhead trout, Oncorhynchus mykiss (Walbaum), from 1977. By histopathology (n = 404), no fish had lesions diagnostic for heart and skeletal muscle inflammation (HSMI). In some groups, lymphohistiocytic endocarditis affected a greater proportion of fish with PRV than fish without PRV, but the range of Ct values among affected fish was within the range of Ct values among unaffected fish. Also, fish with the lowest PRV Ct values (18.4-21.7) lacked endocarditis or any other consistent lesion. From 1987 to 1994, the proportion of PRV positives was not significantly different between farmed Atlantic salmon, Salmo salar L. (44% of 48), and wild-source salmonids (31% of 45). In 2013, the proportion of PRV positives was not significantly different between wild coho salmon, Oncorhynchus kisutch (Walbaum), sampled from British Columbia (5.0% of 60) or the reference region, Alaska, USA (10% of 58). © 2014 John Wiley & Sons Ltd.

  10. Application of neural networks to prediction of fish diversity and salmonid production in the Lake Ontario basin

    USGS Publications Warehouse

    McKenna, James E.

    2005-01-01

    Diversity and fish productivity are important measures of the health and status of aquatic systems. Being able to predict the values of these indices as a function of environmental variables would be valuable to management. Diversity and productivity have been related to environmental conditions by multiple linear regression and discriminant analysis, but such methods have several shortcomings. In an effort to predict fish species diversity and estimate salmonid production for streams in the eastern basin of Lake Ontario, I constructed neural networks and trained them on a data set containing abiotic information and either fish diversity or juvenile salmonid abundance. Twenty percent of the original data were retained as a test data set and used in the training. The ability to extend these neural networks to conditions throughout the streams was tested with data not involved in the network training. The resulting neural networks were able to predict the number of salmonids with more than 84% accuracy and diversity with more than 73% accuracy, which was far superior to the performance of multiple regression. The networks also identified the environmental variables with the greatest predictive power, namely, those describing water movement, stream size, and water chemistry. Thirteen input variables were used to predict diversity and 17 to predict salmonid abundance.

  11. Acoustic Telemetry Evaluation of Juvenile Salmonid Passage and Survival at John Day Dam with Emphasis on the Prototype Surface Flow Outlet, 2008

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Weiland, Mark A.; Ploskey, Gene R.; Hughes, James S.

    The main purpose of the study was to evaluate the performance of Top Spill Weirs installed at two spillbays at John Day Dam and evaluate the effectiveness of these surface flow outlets at attracting juvenile salmon away from the powerhouse and reducing turbine passage. The Juvenile Salmonid Acoustic Telemetry System (JSATS) was used to estimate survival of juvenile salmonids passing the dam and also for calculating performance metrics used to evaluate the efficiency and effectiveness of the dam at passing juvenile salmonids.

  12. Predation by Northern Pikeminnow and tiger muskellunge on juvenile salmonids in a high–head reservoir: Implications for anadromous fish reintroductions

    USGS Publications Warehouse

    Sorel, Mark H.; Hansen, Adam G.; Connelly, Kristin A.; Wilson, Andrew C.; Lowery, Erin D.; Beauchamp, David A.

    2016-01-01

    The feasibility of reintroducing anadromous salmonids into reservoirs above high-head dams is affected by the suitability of the reservoir habitat for rearing and the interactions of the resident fish with introduced fish. We evaluated the predation risk to anadromous salmonids considered for reintroduction in Merwin Reservoir on the North Fork Lewis River in Washington State for two reservoir use-scenarios: year-round rearing and smolt migration. We characterized the role of the primary predators, Northern Pikeminnow Ptychocheilus oregonensis and tiger muskellunge (Northern Pike Esox lucius × Muskellunge E. masquinongy), by using stable isotopes and stomach content analysis, quantified seasonal, per capita predation using bioenergetics modeling, and evaluated the size and age structures of the populations. We then combined these inputs to estimate predation rates of size-structured population units. Northern Pikeminnow of FL ≥ 300 mm were highly cannibalistic and exhibited modest, seasonal, per capita predation on salmonids, but they were disproportionately much less abundant than smaller, less piscivorous, conspecifics. The annual predation on kokanee Oncorhynchus nerka (in biomass) by a size-structured unit of 1,000 Northern Pikeminnow having a FL ≥ 300 mm was analogous to 16,000–40,000 age-0 spring Chinook Salmon O. tshawytscha rearing year-round, or 400–1,000 age-1 smolts migrating April–June. The per capita consumption of salmonids by Northern Pikeminnow having a FL ≥ 200 mm was relatively low, due in large part to spatial segregation during the summer and the skewed size distribution of the predator population. Tiger muskellunge fed heavily on Northern Pikeminnow, other nonsalmonids, and minimally on salmonids. In addition to cannibalism within the Northern Pikeminnow population, predation by tiger muskellunge likely contributed to the low recruitment of larger (more piscivorous) Northern Pikeminnow, thereby decreasing the risk of predation to

  13. [Catering for client groups during the XXII Olympic winter games and XI Paralympic winter games of 2014 in Sochi].

    PubMed

    Popova, A Yu; Gus'kov, A S; Ivanov, G E; Chikina, L V; Klindukhov, V P; Nikolaevich, P N; Grechanaya, T V; Balaeva, M I; Vechernyaya, L S; Vechernyaya, E A; Bozhko, I I; Parkhomenko, V V; Kulichenko, O A; Tushina, O V; Manin, E A; Taran, T V

    2016-01-01

    The problems of catering control various client groups during the XXII Olympic Winter Games and XI Paralympic Winter Games of 2014 in Sochi is one of the priorities of the sanitary and epidemiological welfare of the population during mass events. The data on the order of nutrition of guests and participants of the games, control of food items, sanitary and microbiological monitoring of drinking water, food raw materials and products are presented. It is noted that the ongoing supervisory activities contributed to the sanitary and epidemiological well-being during the Games. The purpose of this study was to lighting modern achievements in the field of nutrition and food microbiology in the period of the Olympic Games and the determination of their value to the further improvement and use at when conducting mass gatherings.

  14. Salmonid Chromosome Evolution as Revealed by a Novel Method for Comparing RADseq Linkage Maps

    PubMed Central

    Gosselin, Thierry; Normandeau, Eric; Lamothe, Manuel; Isabel, Nathalie; Audet, Céline; Bernatchez, Louis

    2016-01-01

    Whole genome duplication (WGD) can provide material for evolutionary innovation. Family Salmonidae is ideal for studying the effects of WGD as the ancestral salmonid underwent WGD relatively recently, ∼65 Ma, then rediploidized and diversified. Extensive synteny between homologous chromosome arms occurs in extant salmonids, but each species has both conserved and unique chromosome arm fusions and fissions. Assembly of large, outbred eukaryotic genomes can be difficult, but structural rearrangements within such taxa can be investigated using linkage maps. RAD sequencing provides unprecedented ability to generate high-density linkage maps for nonmodel species, but can result in low numbers of homologous markers between species due to phylogenetic distance or differences in library preparation. Here, we generate a high-density linkage map (3,826 markers) for the Salvelinus genera (Brook Charr S. fontinalis), and then identify corresponding chromosome arms among the other available salmonid high-density linkage maps, including six species of Oncorhynchus, and one species for each of Salmo, Coregonus, and the nonduplicated sister group for the salmonids, Northern Pike Esox lucius for identifying post-duplicated homeologs. To facilitate this process, we developed MapComp to identify identical and proximate (i.e. nearby) markers between linkage maps using a reference genome of a related species as an intermediate, increasing the number of comparable markers between linkage maps by 5-fold. This enabled a characterization of the most likely history of retained chromosomal rearrangements post-WGD, and several conserved chromosomal inversions. Analyses of RADseq-based linkage maps from other taxa will also benefit from MapComp, available at: https://github.com/enormandeau/mapcomp/ PMID:28173098

  15. Non-native salmonids affect amphibian occupancy at multiple spatial scales

    Treesearch

    David S. Pilliod; Blake R. Hossack; Peter F. Bahls; Evelyn L. Bull; Paul Stephen Corn; Grant Hokit; Bryce A. Maxell; James C. Munger; Aimee Wyrick

    2010-01-01

    The introduction of non-native species into aquatic environments has been linked with local extinctions and altered distributions of native species. We investigated the effect of non-native salmonids on the occupancy of two native amphibians, the long-toed salamander (Ambystoma macrodactylum) and Columbia spotted frog (Rana luteiventris), across three spatial scales:...

  16. Differential invasion success of salmonids in southern Chile: patterns and hypotheses

    USGS Publications Warehouse

    Arismendi, Ivan; Penaluna, Brooke E.; Dunham, Jason B.; García de Leaniz, Carlos; Soto, Doris; Fleming, Ian A.; Gomez-Uchidam, Daniel; Gajardo, Gonzalo; Vargas, Pamela V.; León-Muñoz, Jorge

    2014-01-01

    Biological invasions create complex ecological and societal issues worldwide. Most of the knowledge about invasions comes only from successful invaders, but less is known about which processes determine the differential success of invasions. In this review, we develop a framework to identify the main dimensions driving the success and failure of invaders, including human influences, characteristics of the invader, and biotic interactions. We apply this framework by contrasting hypotheses and available evidence to explain variability in invasion success for 12 salmonids introduced to Chile. The success of Oncorhynchus mykiss and Salmo trutta seems to be influenced by a context-specific combination of their phenotypic plasticity, low ecosystem resistance, and propagule pressure. These well-established invaders may limit the success of subsequently introduced salmonids, with the possible exception of O. tshawytscha, which has a short freshwater residency and limited spatial overlap with trout. Although propagule pressure is high for O. kisutch and S. salar due to their intensive use in aquaculture, their lack of success in Chile may be explained by environmental resistance, including earlier spawning times than in their native ranges, and interactions with previously established and resident Rainbow Trout. Other salmonids have also failed to establish, and they exhibit a suite of ecological traits, environmental resistance, and limited propagule pressure that are variably associated with their lack of success. Collectively, understanding how the various drivers of invasion success interact may explain the differential success of invaders and provide key guidance for managing both positive and negative outcomes associated with their presence.

  17. Migratory Behavior and Survival of Juvenile Salmonids in the Lower Columbia River, Estuary, and Plume in 2010

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McMichael, Geoffrey A.; Harnish, Ryan A.; Skalski, John R.

    Uncertainty regarding the migratory behavior and survival of juvenile salmonids passing through the lower Columbia River and estuary after negotiating dams on the Federal Columbia River Power System (FCRPS) prompted the development and application of the Juvenile Salmon Acoustic Telemetry System (JSATS). The JSATS has been used to investigate the survival of juvenile salmonid smolts between Bonneville Dam (river kilometer (rkm) 236) and the mouth of the Columbia River annually since 2004. In 2010, a total of 12,214 juvenile salmonids were implanted with both a passive integrated transponder (PIT) and a JSATS acoustic transmitter. Using detection information from JSATS receivermore » arrays deployed on dams and in the river, estuary, and plume, the survival probability of yearling Chinook salmon and steelhead smolts tagged at John Day Dam was estimated form multiple reaches between rkm 153 and 8.3 during the spring. During summer, the survival probability of subyearling Chinook salmon was estimated for the same reaches. In addition, the influence of routes of passage (e.g., surface spill, deep spill, turbine, juvenile bypass system) through the lower three dams on the Columbia River (John Day, The Dalles, and Bonneville) on juvenile salmonid smolt survival probability from the dams to rkm 153 and then between rkm 153 and 8.3 was examined to increase understanding of the immediate and latent effects of dam passage on juvenile salmon survival. Similar to previous findings, survival probability was relatively high (>0.95) for most groups of juvenile salmonids from the Bonneville Dam tailrace to about rkm 50. Downstream of rkm 50 the survival probability of all species and run types we examined decreased markedly. Steelhead smolts suffered the highest mortality in this lower portion of the Columbia River estuary, with only an estimated 60% of the tagged fish surviving to the mouth of the river. In contrast, yearling and subyearling Chinook salmon smolts survived to

  18. Cryptic flows: using multiple tracers to relate dissolved oxygen to hyporheic and groundwater flowpaths in intermittent salmonid streams

    NASA Astrophysics Data System (ADS)

    Woelfle-Erskine, C. A.; Larsen, L.; Gomez-Velez, J. D.

    2016-12-01

    Intermittent streams provide important habitat for aquatic species, including endangered salmonid fishes, but during prolonged dry periods may become depleted in dissolved oxygen (DO). The rate of depletion and the consequent length of time a pool remains habitable depend on DO and carbon concentrations in groundwater and hyporheic flow, and within-pool metabolic rates. We performed repeat surveys, habitat characterization, and ecohydrologic sampling on two intermittent tributaries of Salmon Creek (Sonoma Co., CA) to elucidate controls on salmonid over-summer survival at the pool scale. Pools exhibited heterogeneity within and across stream reaches in salmonid recruitment and survival during the summer dry period. In classification tree analysis, high conductivity (>310 mS/cm) and low DO (<2 ppm) were negatively associated with salmonid survival, with high pool conductivity resulting from either groundwater inflow or evapo-concentration. To distinguish between surface, hyporheic, and groundwater contributions, we measured dissolved organic carbon (DOC) concentration and fluorescence excitation-emission matrices (EEMs), radon (222Rn), and stable isotopes (18O and D) in pools, hyporheic flow, and wells and springs in local aquifers. Radon concentrations in pools ranged from 1.5-2.3 Bq/l, 3-4 orders of magnitude higher than expected for water in equilibrium with air, suggesting substantial groundwater inflow. We developed a five-component PARAFAC model from the EEMs and used with the isotope data to perform an end-member mixing analysis to track water sources and flowpaths. These analyses suggested high separability among groundwaters from aquifers separated by faults and between groundwater and surface water, with groundwater of different age and flowpath length discharging to different pools. Pools with shallow groundwater or hyporheic flow sustained DO concentrations above the threshold for salmonid survival, with shallow groundwater unexpectedly acting as a source

  19. Diversity of Flavobacterium psychrophilum and the potential use of its phages for protection against bacterial cold water disease in salmonids.

    PubMed

    Castillo, D; Higuera, G; Villa, M; Middelboe, M; Dalsgaard, I; Madsen, L; Espejo, R T

    2012-03-01

    Flavobacterium psychrophilum causes rainbow trout fry syndrome (RTFS) and cold water disease (CWD) in salmonid aquaculture. We report characterization of F. psychrophilum strains and their bacteriophages isolated in Chilean salmonid aquaculture. Results suggest that under laboratory conditions phages can decrease mortality of salmonids from infection by their F. psychrophilum host strain. Twelve F. psychrophilum isolates were characterized, with DNA restriction patterns showing low diversity between strains despite their being obtained from different salmonid production sites and from different tissues. We isolated 15 bacteriophages able to infect some of the F. psychrophilum isolates and characterized six of them in detail. DNA genome sizes were close to 50 Kbp and corresponded to the Siphoviridae and Podoviridae families. One isolate, 6H, probably contains lipids as an essential virion component, based on its chloroform sensitivity and low buoyant density in CsCl. Each phage isolate rarely infected F. psychrophilum strains other than the strain used for its enrichment and isolation. Some bacteriophages could decrease mortality from intraperitoneal injection of its host strain when added together with the bacteria in a ratio of 10 plaque-forming units per colony-forming unit. While we recognize the artificial laboratory conditions used for these protection assays, this work is the first to demonstrate that phages might be able protect salmonids from RTFS or CWD. © 2012 Blackwell Publishing Ltd.

  20. System-Wide Significance of Predation on Juvenile Salmonids in Columbia and Snake River Reservoirs : Annual Report 1992.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Petersen, James H.; Poe, Thomas P.

    1993-12-01

    Northern squawfish (Ptychocheilus oregonensis) predation on juvenile salmonids was characterized during 1992 at ten locations in the Columbia River below Bonneville Dam and at three locations in John Day Reservoir. During the spring and summer, 1,487 northern squawfish were collected in the lower Columbia River and 202 squawfish were sampled in John Day Reservoir. Gut content data, predator weight, and water temperature were used to compute a consumption index (CI) for northern squawfish, and overall diet was also described. In the Columbia River below Bonneville Dam, northern squawfish diet was primarily fish (spring 69%; summer 53%), most of which weremore » salmonids. Salmonids were also the primary diet component in the Bonneville Dam tailrace, John Day Dam forebay, and the McNary Dam tailrace. Crustaceans were the dominant diet item at the John Day mid-reservoir location, although sample sizes were small. About half of the non-salmonid preyfish were sculpins. The consumption index (CI) of northern squawfish was generally higher during summer than during spring. The highest CI`s were observed during summer in the tailrace boat restricted zones of Bonneville Dam (CI = 7.8) and McNary Dam (CI = 4.6). At locations below Bonneville Dam, CI`s were relatively low near Covert`s Landing and Rooster Rock, higher at four locations between Blue Lake and St. Helens, and low again at three downriver sites (Kalama, Ranier, and Jones Beach). Northern squawfish catches and CI`s were noticeably higher throughout the lower Columbia compared to mid-reservoir sites further upriver sampled during 1990--92. Predation may be especially intense in the free-flowing section of the Columbia River below Bonneville Dam. Smallmouth bass (Micropterus dolomieui; N = 198) ate mostly fish -- 25% salmonids, 29% sculpins, and 46% other fish. Highest catches of smallmouth bass were in the John Day Dam forebay.« less

  1. Formation of Biphasic Hydroxylapatite-Beta Magnesium Tricalcium Phosphate in Heat Treated Salmonid Vertebrae.

    PubMed

    Butler, Don H; Shahack-Gross, Ruth

    2017-06-15

    Ichthyoarchaeological evidence is uncommon at ancient hunter-gatherer sites from various regions and timeframes. This research contributes to the development of microarchaeological techniques useful for identifying fishing economies in situations where classifiable bones are unavailable. Specifically, traces of heat altered bone mineral in domestic hearths are expected to provide markers for discarded fish remains. We used a series of laboratory incineration experiments to characterize the mineralogy of burned salmonid vertebrae. Fourier transform infrared spectroscopy and x-ray diffraction distinguished the formation of beta magnesium tricalcium phosphate (βMgTCP) at temperatures as low as 600 °C. Bones from a sample of game mammals and birds did not form this phase at temperatures below 1,000 °C. We propose that this neoformed mineral can serve as a proxy for hunter-gatherer salmonid fishing when typical ichthyoarchaeological evidence is absent. Using Fourier transform infrared spectroscopy, it will be possible to rapidly and inexpensively determine the presence of βMgTCP in fragmentary burned bone remains associated with combustion features. The occurrence of βMgTCP in archaeological hearth features will offer a new means of further evaluating the temporal, geographic, and cultural scope of salmonid harvesting. We also acknowledge the value of biphasic hydroxylapatite-βMgTCP recovered from Atlantic salmon vertebrae as a bioceramic.

  2. [Improving prevention activities of infectious diseases during preparation and holding of the XXII Olympic Winter Games and XI Paralympic Winter Games 2014 in Sochi].

    PubMed

    Onishchenko, G G; Bragina, I V; Ezhlova, E B; Demina, Iu V; Grechanaia, T V; Nikolaevich, P N; Balaeva, M I; Tesheva, S Ch; Biriukov, V A; Kulichenko, A N; Vasilenko, N F; Maletskaia, O V; Manin, E A; Orobeĭ, V G

    2015-01-01

    The article presents data on the implementation of a set of preventive activities to ensure sanitation and epidemiological welfare during the XXII Olympic Winter Games and XI Paralympic Winter Games 2014 in Sochi. The importance of monitoring and evaluation of epidemiological risk, as the basis of formation of preventive measures is noticed. The questions of specific, and nonspecific prevention of infectious diseases, especially the work done during the pre-Olympic period are considered. The importance of specifically developed regulatory basis, and health education are emphasized. The conclusion about the effectiveness of the measures taken, which led to a significant reduction of infectious diseases in the region is made.

  3. Novel RAD sequence data reveal a lack of genomic divergence between dietary ecotypes in a landlocked salmonid population

    USGS Publications Warehouse

    Limborg, Morten T.; Larson, Wesley; Shedd, Kyle; Seeb, Lisa W.; Seeb, James E.

    2017-01-01

    Preservation of heritable ecological diversity within species and populations is a key challenge for managing natural resources and wild populations. Salmonid fish are iconic and socio-economically important species for commercial, aquaculture, and recreational fisheries across the globe. Many salmonids are known to exhibit ecological divergence within species, including distinct feeding ecotypes within the same lakes. Here we used 5559 SNPs, derived from RAD sequencing, to perform population genetic comparisons between two dietary ecotypes of sockeye salmon (Oncorhynchus nerka) in Jo-Jo Lake, Alaska (USA). We tested the standing hypothesis that these two ecotypes are currently diverging as a result of adaptation to distinct dietary niches; results support earlier conclusions of a single panmictic population. The RAD sequence data revealed 40 new SNPs not previously detected in the species, and our sequence data can be used in future studies of ecotypic diversity in salmonid species.

  4. Comparison of growth and metabolic regulation between wild, domesticated and transgenic salmonids.

    USDA-ARS?s Scientific Manuscript database

    To gain a better understanding of the aspects underlying normal and growth hormone enhanced growth in salmonids, quantitative expression analysis was performed for a number of genes related to muscle growth, metabolism, immunology and energy regulation. This analysis was performed in liver and musc...

  5. A permeability study on salmonid spawning areas in northern Humboldt County, California

    Treesearch

    Claire Knopf

    2012-01-01

    This research was conducted to determine if local salmonid, specifically coho salmon (Oncorhynchus kisutch), Chinook salmon (O. tshawytscha), and the steelhead trout (O. mykiss), redd location is dependent upon the permeability of the streambed. The study took place at four study sites in two coastal...

  6. How do we know how many salmon returned to spawn? Implementing the California Coastal salmonid monitoring plan in Mendocino County, California

    Treesearch

    Sean P. Gallagher; David W. Wright

    2012-01-01

    California's coastal salmon and steelhead populations are listed under California and Federal Endangered Species Acts; both require monitoring to provide measures of recovery. Since 2004 the California Department of Fish and Game and NOAA Fisheries have been developing a monitoring plan for California¡¯s coastal salmonids (the California Coastal Salmonid...

  7. Sluiceway Operations to Pass Juvenile Salmonids at The Dalles Dam, Columbia River, USA

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Johnson, Gary E.; Khan, Fenton; Skalski, J. R.

    Existing ice and trash sluiceways are commonly used to pass juvenile salmonids downstream at hydropower dams through a benign, non-turbine route. At The Dalles Dam on the Columbia River, managers undertook optimizing operations of sluiceway weirs to maximize survival of juvenile salmonids at the powerhouse. We applied fixed-location hydroacoustic methods to compare fish passage rates and sluiceway efficiencies for two weir configurations during 2004 and 2005: three weirs versus six weirs, located at the mid- versus east powerhouse, respectively. We also analyzed horizontal distributions of passage at the sluiceway and turbines and the effects of operating turbines beneath open sluicewaymore » gates to provide supporting data relevant to operations optimization. Based on the findings, we recommend the following for long-term operations for the sluiceway at The Dalles Dam: open six rather than three sluiceway weirs to take advantage of the maximum hydraulic capacity of the sluiceway; open the three weirs above the western-most operating main turbine unit (MU) and the three weirs at MU 8 where turbine passage rates are relatively high; operate the turbine units below open sluiceway weirs as a standard procedure; operate the sluiceway 24 h/d year-round to maximize its benefits to juvenile salmonids; and use the same operations for spring and summer emigrants. These operational concepts are transferable to dams where sluiceway surface flow outlets are used protect downstream migrating fishes.« less

  8. Comparing stream-specific to generalized temperature models to guide salmonid management in a changing climate

    USGS Publications Warehouse

    Andrew K. Carlson,; William W. Taylor,; Hartikainen, Kelsey M.; Dana M. Infante,; Beard, Douglas; Lynch, Abigail

    2017-01-01

    Global climate change is predicted to increase air and stream temperatures and alter thermal habitat suitability for growth and survival of coldwater fishes, including brook charr (Salvelinus fontinalis), brown trout (Salmo trutta), and rainbow trout (Oncorhynchus mykiss). In a changing climate, accurate stream temperature modeling is increasingly important for sustainable salmonid management throughout the world. However, finite resource availability (e.g. funding, personnel) drives a tradeoff between thermal model accuracy and efficiency (i.e. cost-effective applicability at management-relevant spatial extents). Using different projected climate change scenarios, we compared the accuracy and efficiency of stream-specific and generalized (i.e. region-specific) temperature models for coldwater salmonids within and outside the State of Michigan, USA, a region with long-term stream temperature data and productive coldwater fisheries. Projected stream temperature warming between 2016 and 2056 ranged from 0.1 to 3.8 °C in groundwater-dominated streams and 0.2–6.8 °C in surface-runoff dominated systems in the State of Michigan. Despite their generally lower accuracy in predicting exact stream temperatures, generalized models accurately projected salmonid thermal habitat suitability in 82% of groundwater-dominated streams, including those with brook charr (80% accuracy), brown trout (89% accuracy), and rainbow trout (75% accuracy). In contrast, generalized models predicted thermal habitat suitability in runoff-dominated streams with much lower accuracy (54%). These results suggest that, amidst climate change and constraints in resource availability, generalized models are appropriate to forecast thermal conditions in groundwater-dominated streams within and outside Michigan and inform regional-level salmonid management strategies that are practical for coldwater fisheries managers, policy makers, and the public. We recommend fisheries professionals reserve resource

  9. DEVELOPMENT OF FIELD-BASED EMPIRICAL MODELS OF SUITABLE TEMPERATURE REGIMES FOR INTERIOR SALMONIDS

    EPA Science Inventory

    Interior salmonids are species of growing interest and concern in the Pacific Northwest. Evidence of population declines associated with habitat loss and fragmentation have culminate in every species being listed, or proposed or petitioned for listing under he Endangered Species...

  10. A Synthesis of Tagging Studies Examining the Behaviour and Survival of Anadromous Salmonids in Marine Environments

    PubMed Central

    Drenner, S. Matthew; Clark, Timothy D.; Whitney, Charlotte K.; Martins, Eduardo G.; Cooke, Steven J.; Hinch, Scott G.

    2012-01-01

    This paper synthesizes tagging studies to highlight the current state of knowledge concerning the behaviour and survival of anadromous salmonids in the marine environment. Scientific literature was reviewed to quantify the number and type of studies that have investigated behaviour and survival of anadromous forms of Pacific salmon (Oncorhynchus spp.), Atlantic salmon (Salmo salar), brown trout (Salmo trutta), steelhead (Oncorhynchus mykiss), and cutthroat trout (Oncorhynchus clarkii). We examined three categories of tags including electronic (e.g. acoustic, radio, archival), passive (e.g. external marks, Carlin, coded wire, passive integrated transponder [PIT]), and biological (e.g. otolith, genetic, scale, parasites). Based on 207 papers, survival rates and behaviour in marine environments were found to be extremely variable spatially and temporally, with some of the most influential factors being temperature, population, physiological state, and fish size. Salmonids at all life stages were consistently found to swim at an average speed of approximately one body length per second, which likely corresponds with the speed at which transport costs are minimal. We found that there is relatively little research conducted on open-ocean migrating salmonids, and some species (e.g. masu [O. masou] and amago [O. rhodurus]) are underrepresented in the literature. The most common forms of tagging used across life stages were various forms of external tags, coded wire tags, and acoustic tags, however, the majority of studies did not measure tagging/handling effects on the fish, tag loss/failure, or tag detection probabilities when estimating survival. Through the interdisciplinary application of existing and novel technologies, future research examining the behaviour and survival of anadromous salmonids could incorporate important drivers such as oceanography, tagging/handling effects, predation, and physiology. PMID:22431962

  11. A synthesis of tagging studies examining the behaviour and survival of anadromous salmonids in marine environments.

    PubMed

    Drenner, S Matthew; Clark, Timothy D; Whitney, Charlotte K; Martins, Eduardo G; Cooke, Steven J; Hinch, Scott G

    2012-01-01

    This paper synthesizes tagging studies to highlight the current state of knowledge concerning the behaviour and survival of anadromous salmonids in the marine environment. Scientific literature was reviewed to quantify the number and type of studies that have investigated behaviour and survival of anadromous forms of Pacific salmon (Oncorhynchus spp.), Atlantic salmon (Salmo salar), brown trout (Salmo trutta), steelhead (Oncorhynchus mykiss), and cutthroat trout (Oncorhynchus clarkii). We examined three categories of tags including electronic (e.g. acoustic, radio, archival), passive (e.g. external marks, Carlin, coded wire, passive integrated transponder [PIT]), and biological (e.g. otolith, genetic, scale, parasites). Based on 207 papers, survival rates and behaviour in marine environments were found to be extremely variable spatially and temporally, with some of the most influential factors being temperature, population, physiological state, and fish size. Salmonids at all life stages were consistently found to swim at an average speed of approximately one body length per second, which likely corresponds with the speed at which transport costs are minimal. We found that there is relatively little research conducted on open-ocean migrating salmonids, and some species (e.g. masu [O. masou] and amago [O. rhodurus]) are underrepresented in the literature. The most common forms of tagging used across life stages were various forms of external tags, coded wire tags, and acoustic tags, however, the majority of studies did not measure tagging/handling effects on the fish, tag loss/failure, or tag detection probabilities when estimating survival. Through the interdisciplinary application of existing and novel technologies, future research examining the behaviour and survival of anadromous salmonids could incorporate important drivers such as oceanography, tagging/handling effects, predation, and physiology.

  12. Antimicrobial and host cell-directed activities of Gly/Ser-rich peptides from salmonid cathelicidins.

    PubMed

    D'Este, Francesca; Benincasa, Monica; Cannone, Giuseppe; Furlan, Michela; Scarsini, Michele; Volpatti, Donatella; Gennaro, Renato; Tossi, Alessandro; Skerlavaj, Barbara; Scocchi, Marco

    2016-12-01

    Cathelicidins, a major family of vertebrate antimicrobial peptides (AMPs), have a recognized role in the first line of defense against infections. They have been identified in several salmonid species, where the putative mature peptides are unusually long and rich in serine and glycine residues, often arranged in short multiple repeats (RLGGGS/RPGGGS) intercalated by hydrophobic motifs. Fragments of 24-40 residues, spanning specific motifs and conserved sequences in grayling or brown, rainbow and brook trout, were chemically synthesized and examined for antimicrobial activity against relevant Gram-positive and Gram-negative salmonid pathogens, as well as laboratory reference strains. They were not active in complete medium, but showed varying potency and activity spectra in diluted media. Bacterial membrane permeabilization also occurred only under these conditions and was indicated by rapid propidium iodide uptake in peptide-treated bacteria. However, circular dichroism analyses indicated that they did not significantly adopt ordered conformations in membrane-like environments. The peptides were not hemolytic or cytotoxic to trout cells, including freshly purified head kidney leukocytes (HKL) and the fibroblastic RTG-2 cell line. Notably, when exposed to them, HKL showed increased metabolic activity, while a growth-promoting effect was observed on RTG-2 cells, suggesting a functional interaction of salmonid cathelicidins with host cells similar to that shown by mammalian ones. The three most active peptides produced a dose-dependent increase in phagocytic uptake by HKL simultaneously stimulated with bacterial particles. The peptide STF(1-37), selected for further analyses, also enhanced phagocytic uptake in the presence of autologous serum, and increased intracellular killing of live E. coli. Furthermore, when tested on HKL in combination with the immunostimulant β-glucan, it synergistically potentiated both phagocytic uptake and the respiratory burst response

  13. Prevalence of infectious salmon anaemia virus (ISAV) in wild salmonids in western Norway.

    PubMed

    Plarre, H; Devold, M; Snow, M; Nylund, A

    2005-08-09

    Studies of infectious salmon anaemia virus (ISAV), an important pathogen of farmed salmon in Norway, Scotland, the Faeroe Islands, Ireland, Canada, the USA and Chile, suggest that natural reservoirs for this virus can be found on both sides of the North Atlantic. Based on existing information about ISAV it is believed to be maintained in wild populations of trout and salmon in Europe. It has further been suggested that ISAV is transmitted between wild hosts, mainly during their freshwater spawning phase in rivers, and that wild salmonids, mainly trout, are possible carriers of benign wild-type variants of ISAV. Change in virulence is probably a result of deletions of amino acid segments from the highly polymorphic region (HPR) of benign wild-type isolates after transmission to farmed salmon. Hence, it has been suggested that the frequency of new outbreaks of ISA in farmed salmon could partly reflect natural variation in the prevalence of ISAV in wild populations of salmonids. The aims of the present study were to screen for ISAV in wild salmonids during spawning in rivers and to determine the pathogenicity of resultant isolates from wild fish. Tissues from wild salmonids were screened by RT-PCR and real-time PCR. The prevalence of ISAV in wild trout Salmo trutta varied from 62 to 100% between tested rivers in 2001. The prevalence dropped in 2002, ranging from 13 to 36% in the same rivers and to only 6% in 2003. All ISAV were nonpathogenic when injected into disease-free Atlantic salmon, but were capable of propagation, as indicated by subsequent viral recovery. However, non-pathogenic ISAV has also been found in farmed salmon, where a prevalence as high as 60% has been registered, but with no mortalities occurring. Based on the results of the present and other studies, it must be concluded that vital information about the importance of wild and man-made reservoirs for the emergence of ISA in salmon farming is still lacking. This information can only be gained by

  14. Targeted sequencing for high-resolution evolutionary analyses following genome duplication in salmonid fish: Proof of concept for key components of the insulin-like growth factor axis.

    PubMed

    Lappin, Fiona M; Shaw, Rebecca L; Macqueen, Daniel J

    2016-12-01

    High-throughput sequencing has revolutionised comparative and evolutionary genome biology. It has now become relatively commonplace to generate multiple genomes and/or transcriptomes to characterize the evolution of large taxonomic groups of interest. Nevertheless, such efforts may be unsuited to some research questions or remain beyond the scope of some research groups. Here we show that targeted high-throughput sequencing offers a viable alternative to study genome evolution across a vertebrate family of great scientific interest. Specifically, we exploited sequence capture and Illumina sequencing to characterize the evolution of key components from the insulin-like growth (IGF) signalling axis of salmonid fish at unprecedented phylogenetic resolution. The IGF axis represents a central governor of vertebrate growth and its core components were expanded by whole genome duplication in the salmonid ancestor ~95Ma. Using RNA baits synthesised to genes encoding the complete family of IGF binding proteins (IGFBP) and an IGF hormone (IGF2), we captured, sequenced and assembled orthologous and paralogous exons from species representing all ten salmonid genera. This approach generated 299 novel sequences, most as complete or near-complete protein-coding sequences. Phylogenetic analyses confirmed congruent evolutionary histories for all nineteen recognized salmonid IGFBP family members and identified novel salmonid-specific IGF2 paralogues. Moreover, we reconstructed the evolution of duplicated IGF axis paralogues across a replete salmonid phylogeny, revealing complex historic selection regimes - both ancestral to salmonids and lineage-restricted - that frequently involved asymmetric paralogue divergence under positive and/or relaxed purifying selection. Our findings add to an emerging literature highlighting diverse applications for targeted sequencing in comparative-evolutionary genomics. We also set out a viable approach to obtain large sets of nuclear genes for any

  15. An approach to effectiveness monitoring of floodplain channel aquatic habitat: salmonid relationships.

    Treesearch

    M.D. Bryant; R.T. Edwards; R.D. Woodsmith

    2005-01-01

    Rivers and streams that support anadromous salmonids are an important part of land management planning in southeastern Alaska and the Pacific Northwest of North America. Land managers and planners require a consistent set of protocols that include both the physical and biological aspects of the stream for effectiveness monitoring procedures to evaluate management...

  16. A simple method for in situ monitoring of water temperature in substrates used by spawning salmonids

    USGS Publications Warehouse

    Zimmerman, Christian E.; Finn, James E.

    2012-01-01

    Interstitial water temperature within spawning habitats of salmonids may differ from surface-water temperature depending on intragravel flow paths, geomorphic setting, or presence of groundwater. Because survival and developmental timing of salmon are partly controlled by temperature, monitoring temperature within gravels used by spawning salmonids is required to adequately describe the environment experienced by incubating eggs and embryos. Here we describe a simple method of deploying electronic data loggers within gravel substrates with minimal alteration of the natural gravel structure and composition. Using data collected in spawning sites used by summer and fall chum salmon Oncorhynchus keta from two streams within the Yukon River watershed, we compare contrasting thermal regimes to demonstrate the utility of this method.

  17. The role of emergent wetlands as potential rearing habitats for juvenile salmonids

    USGS Publications Warehouse

    Henning, Julie A.; Gresswell, Robert E.; Flemming, Ian A.

    2006-01-01

    A recent trend of enhancing freshwater emergent wetlands for waterfowl and other wildlife has raised concern about the effects of such measures on juvenile salmonids. We undertook this study to quantify the degree and extent of juvenile Pacific salmon Oncorhynchus spp. utilization of enhanced and unenhanced emergent wetlands within the floodplain of the lower Chehalis River, Washington, and to determine the fate of the salmon using them. Enhanced emergent wetlands contained water control structures that provided an outlet for fish emigration and a longer hydroperiod for rearing than unenhanced wetlands. Age-0 and age-1 coho salmon O. kisutch were the most common salmonid at all sites, enhanced wetlands having significantly higher age-1 abundance than unenhanced wetlands that were a similar distance from the main-stem river. Yearling coho salmon benefited from rearing in two enhanced wetland habitats, where their specific growth rate and minimum estimates of survival (1.43%/d by weight and 30%; 1.37%/d and 57%) were comparable to those in other side-channel rearing studies. Dissolved oxygen concentrations decreased in emergent wetlands throughout the season and approached the limits lethal to juvenile salmon by May or June each year. Emigration patterns suggested that age-0 and age-1 coho salmon emigrated as habitat conditions declined. This observation was further supported by the results of an experimental release of coho salmon. Survival of fish utilizing emergent wetlands was dependent on movement to the river before water quality decreased or stranding occurred from wetland desiccation. Thus, our results suggest that enhancing freshwater wetlands via water control structures can benefit juvenile salmonids, at least in the short term, by providing conditions for greater growth, survival, and emigration.

  18. [Modern methods application of genotyping of infectious diseases pathogens in the context of operational work of specialized anti-epidemic team during the XXII Olympic Winter Games and XI Paralympic Winter Games].

    PubMed

    Kuzkin, B P; Kulichenko, A N; Volynkina, A S; Efremenko, D V; Kuznetsova, I V; Kotenev, E S; Lyamkin, G I; Kartsev, N N; Klindukhov, V P

    2015-01-01

    This paper considers the experience of genotyping and sequencing technologies in laboratories of specialized anti-epidemic team (SAET) during the XXII Olympic Winter Games and XI Paralympic Winter Games of 2014 in Sochi. The work carried out during the pre-Olympic period on performance of readiness by SAET for these studies is analyzed. The results of genotyping strains of pathogens during the Olympic Games are presented. A conclusion about the effectiveness of the use of molecular genetic techniques in terms of SAET is made.

  19. Juvenile salmonid monitoring in the White Salmon River, Washington, post-Condit Dam removal, 2016

    USGS Publications Warehouse

    Jezorek, Ian G.; Hardiman, Jill M.

    2017-06-23

    Condit Dam, at river kilometer 5.3 on the White Salmon River, Washington, was breached in 2011 and removed completely in 2012, allowing anadromous salmonids access to habitat that had been blocked for nearly 100 years. A multi-agency workgroup concluded that the preferred salmonid restoration alternative was natural recolonization with monitoring to assess efficacy, followed by a management evaluation 5 years after dam removal. Limited monitoring of salmon and steelhead spawning has occurred since 2011, but no monitoring of juveniles occurred until 2016. During 2016, we operated a rotary screw trap at river kilometer 2.3 (3 kilometers downstream of the former dam site) from late March through May and used backpack electrofishing during summer to assess juvenile salmonid distribution and abundance. The screw trap captured primarily steelhead (Oncorhynchus mykiss; smolts, parr, and fry) and coho salmon (O. kisutch; smolts and fry). We estimated the number of steelhead smolts at 3,851 (standard error = 1,454) and coho smolts at 1,093 (standard error = 412). In this document, we refer to O. mykiss caught at the screw trap as steelhead because they were actively migrating, but because we did not know migratory status of O. mykiss caught in electrofishing surveys, we simply refer to them as O. mykiss or steelhead/rainbow trout. Steelhead and coho smolts tagged with passive integrated transponder tags were subsequently detected downstream at Bonneville Dam on the Columbia River. Few Chinook salmon (O. tshawytscha) fry were captured, possibly as a result of trap location or effects of a December 2015 flood. Sampling in Mill, Buck, and Rattlesnake Creeks (all upstream of the former dam site) showed that juvenile coho were present in Mill and Buck Creeks, suggesting spawning had occurred there. We compared O. mykiss abundance data in sites on Buck and Rattlesnake Creeks to pre-dam removal data. During 2016, age-0 O. mykiss were more abundant in Buck Creek than in 2009 or

  20. Tolerance of developing salmonid eggs and fry to nitrate exposure

    USGS Publications Warehouse

    Kincheloe, John W.; Wedemeyer, Gary A.; Koch, David L.

    1979-01-01

    This paper reports on tests which show significant effects on early salmonid life stages of nitrates at levels commonly found in groundwaters in geographical areas that are influenced by fertilizer application. It has long been known, from fish cultural experience, that in certain site specific locations, chronic problems can be expected with salmonid egg development and early fry mortality. However, fingerlings which survive usually grow normally. A complete explanation is lacking although several environmental factors have been proposed to account for this phenomenon. One, which has so far received little attention, is that nitrate levels in the ground and surface waters of many areas have been increasing significantly over historical background levels. Ammonia, urea, and other potential sources of nitrate can enter natural waters from a variety of sources, such as domestic or industrial sewage, animal feedlots, or seepage and return flows from agricultural lands. The latter may be the largest contributor, since billions of tons of nitrate fertilizers are applied to agricultural crops on a worldwide basis each year. In addition, intensive forest management techniques include the aerial application of nitrate fertilizer to increase the yield of wood products, while range management practices call for use of nitrates to increase forage production. The nitrate that is not taken up by plants ultimately appears in ground or surface waters.

  1. Salmon lice – impact on wild salmonids and salmon aquaculture

    PubMed Central

    Torrissen, O; Jones, S; Asche, F; Guttormsen, A; Skilbrei, O T; Nilsen, F; Horsberg, T E; Jackson, D

    2013-01-01

    Salmon lice, Lepeophtheirus salmonis, are naturally occurring parasites of salmon in sea water. Intensive salmon farming provides better conditions for parasite growth and transmission compared with natural conditions, creating problems for both the salmon farming industry and, under certain conditions, wild salmonids. Salmon lice originating from farms negatively impact wild stocks of salmonids, although the extent of the impact is a matter of debate. Estimates from Ireland and Norway indicate an odds ratio of 1.1:1-1.2:1 for sea lice treated Atlantic salmon smolt to survive sea migration compared to untreated smolts. This is considered to have a moderate population regulatory effect. The development of resistance against drugs most commonly used to treat salmon lice is a serious concern for both wild and farmed fish. Several large initiatives have been taken to encourage the development of new strategies, such as vaccines and novel drugs, for the treatment or removal of salmon lice from farmed fish. The newly sequenced salmon louse genome will be an important tool in this work. The use of cleaner fish has emerged as a robust method for controlling salmon lice, and aquaculture production of wrasse is important towards this aim. Salmon lice have large economic consequences for the salmon industry, both as direct costs for the prevention and treatment, but also indirectly through negative public opinion. PMID:23311858

  2. [ORGANIZATION OF THE QUALITY CONTROL OF PLACEMENT AND ACCOMMODATION OF PARTICIPANTS ATTENDANTS AND GUESTS OF THE XXII OLYMPIC WINTER GAMES AND XI PARALYMPIC WINTER GAMES OF 2014 IN THE RESORT CITY OF SOCHI].

    PubMed

    Gorskiĭ, A A; Gus'kov, A S; Pochtareva, E S; Klindukhov, V P; Nikolaevich, P N; Grechanaia, T V; Vechemyaia, E A; Biriukov, V A; Bozhko, I I; Kulichenko, A N; Taran, T V; Zaĭtsev, A A; Tushina, O V

    2015-01-01

    There is presented the analysis of activities of the Federal Service for Supervision of Consumer Rights protection and Human Welfare to ensure adequate conditions of accommodation of the participants, attendants and guests of the XXII Olympic Winter Games and XI Paralympic Winter Games of 2014 in the Resort city of Sochi according to regulated requirements. There were detected ways of the strengthening the supervision for the quality of the accommodation during mass sports activities for the assurance of the rights for consumers.

  3. The potential impacts of migratory difficulty, including warmer waters and altered flow conditions, on the reproductive success of salmonid fishes.

    PubMed

    Fenkes, Miriam; Shiels, Holly A; Fitzpatrick, John L; Nudds, Robert L

    2016-03-01

    Climate change and urbanisation of watercourses affect water temperatures and current flow velocities in river systems on a global scale. This represents a particularly critical issue for migratory fish species with complex life histories that use rivers to reproduce. Salmonids are migratory keystone species that provide substantial economical value to ecosystems and human societies. Consequently, a comprehensive understanding of the effects of environmental stressors on their reproductive success is critical in order to ensure their continued abundance during future climatic change. Salmonids are capital breeders, relying entirely on endogenous energy stores to fuel return migration to their natal spawning sites and reproduction upon arrival. Metabolic rates and cost of transport en-route increase with temperature and at extreme temperatures, swimming is increasingly fuelled anaerobically, resulting in an oxygen debt and reduced capacity to recover from exhaustive exercise. Thermally challenged salmonids also produce less viable gametes, which themselves are affected by water temperature after release. Passage through hydrological barriers and temperature changes both affect energy expenditure. As a result, important energetic tradeoffs emerge between extra energy used during migration and that available for other facets of the reproductive cycle, such as reproductive competition and gamete production. However, studies identifying these tradeoffs are extremely sparse. This review focuses on the specific locomotor responses of salmonids to thermal and hydrological challenges, identifying gaps in our knowledge and highlighting the potential implications for key aspects of their reproduction. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

  4. The potential impacts of migratory difficulty, including warmer waters and altered flow conditions, on the reproductive success of salmonid fishes

    PubMed Central

    Fenkes, Miriam; Shiels, Holly A.; Fitzpatrick, John L.; Nudds, Robert L.

    2016-01-01

    Climate change and urbanisation of watercourses affect water temperatures and current flow velocities in river systems on a global scale. This represents a particularly critical issue for migratory fish species with complex life histories that use rivers to reproduce. Salmonids are migratory keystone species that provide substantial economical value to ecosystems and human societies. Consequently, a comprehensive understanding of the effects of environmental stressors on their reproductive success is critical in order to ensure their continued abundance during future climatic change. Salmonids are capital breeders, relying entirely on endogenous energy stores to fuel return migration to their natal spawning sites and reproduction upon arrival. Metabolic rates and cost of transport en-route increase with temperature and at extreme temperatures, swimming is increasingly fuelled anaerobically, resulting in an oxygen debt and reduced capacity to recover from exhaustive exercise. Thermally challenged salmonids also produce less viable gametes, which themselves are affected by water temperature after release. Passage through hydrological barriers and temperature changes both affect energy expenditure. As a result, important energetic tradeoffs emerge between extra energy used during migration and that available for other facets of the reproductive cycle, such as reproductive competition and gamete production. However, studies identifying these tradeoffs are extremely sparse. This review focuses on the specific locomotor responses of salmonids to thermal and hydrological challenges, identifying gaps in our knowledge and highlighting the potential implications for key aspects of their reproduction. PMID:26603555

  5. Barriers, invasion, and conservation of native salmonids in coldwater streams [Box 18.2

    Treesearch

    Bruce Rieman; Michael Young; Kurt Fausch; Jason Dunham; Douglas Peterson

    2010-01-01

    Habitat loss and fragmentation are threats to persistence of many native fish populations. Invading nonnative species that may restrict or displace native species are also important. These two issues are particularly relevant for native salmonids that are often limited to remnant habitats in cold, headwater streams. On the surface, reversing threats to native fishes...

  6. Some metabolic effects of bacterial endotoxins in salmonid fishes

    USGS Publications Warehouse

    Wedemeyer, G.A.; Ross, A.J.; Smith, L.

    1968-01-01

    Coho salmon (Oncorhynchus kisutch) and rainbow trout (Salmo gairdneri) were highly resistant to endotoxins from both Escherichia coli and Aeromonas salmonicida (a fish pathogen) at 14 and 18 C.This resistance was investigated with liver tryptophan pyrrolase, liver glycogen depletion in vitro, and the arterial blood pressure as indicators. Liver glycogen depletion was accelerated by both endotoxins, but there was no significant cardiovascular response or effect on liver tryptophan pyrrolase activity. Since the cardiovascular effects of histamine were also limited, it was concluded that the metabolic effects of bacterial endotoxins in salmonids are qualitatively different from those of the higher vertebrates.

  7. 23RD International Conference on Phenomena in Ionized Gases, Volume 2

    DTIC Science & Technology

    1998-12-01

    able voltage arcs and thermoionic converters [10]. The news for subsequent evolution into a prebiotic structure, XXIII ICPIG (Toulouse, France ) 17...possibilities of a prebiotic [9]. M.Sanduloviciu, Proc XXII ICPIG New Jersey structure in the early Earth atmosphere (a plasma like 1995, Contr. Paper 1, p

  8. Effects of Catch-and-Release Angling on Salmonids at Elevated Water Temperatures

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Boyd, James W.; Guy, Christopher S.; Horton, Travis

    2010-08-01

    Few studies have assessed catch and release mortality of salmonids at water temperatures ≥23°C, despite predictions of warming stream temperatures due to climate change. In addition, the effects of diel temperature fluctuations on salmonid mortality have largely been ignored in catch and release angling studies. The primary objective of this study was to measure catch and release mortality of rainbow trout Oncorhynchus mykiss, brown trout Salmo trutta, and mountain whitefish Prosopium williamsoni in three water temperature treatments; when daily maximum water temperatures were cool (<20°C), warm (20 to 22.9°C), and hot ( 23°C). A secondary objective was to assess catchmore » and release mortality of salmonids angled in morning and evening within water-temperature treatments. These objectives were related to Montana Fish, Wildlife and Parks’ Drought Fishing Closure Policy (DFCP). Angling (fly-fishing only) occurred in the Gallatin and Smith rivers. All angled fish were confined to in-stream holding cages and monitored for mortality for 72 h. Mortality of rainbow trout peaked at 16% in the Gallatin River and 9% in the Smith River during the hot treatment. Mortality of brown trout was less than 5% in all water-temperature treatments in both rivers. Mountain whitefish mortality peaked at 28% in the hot treatment in the Smith River. No mortality for any species occurred in either river when daily maximum water temperatures were <20°C. Mortality of rainbow trout peaked at 16% in the evening hot treatment in the Smith River. Mortality of brown trout and mountain whitefish was not related to time of day. The catch and release mortality values presented here likely represent fishing mortality given that most anglers in southwest Montana practice catch and release angling. The mortality values we observed were lower than predicted (< 30%), given reports in the literature. The difference is likely related to the in situ nature of the study and periods of cooler

  9. Differential predation by northern squawfish Ptychocheilus oregonensis on live and dead juvenile salmonids in the Bonneville Dam tailrace (Columbia River)

    USGS Publications Warehouse

    Petersen, James H.; Gadomski, Dena M.; Poe, Thomas P.

    1994-01-01

    Juvenile salmonids (Oncorhynchus spp.) that have been killed or injured during dam passage may be highly vulnerable or preferred prey of predators that aggregate below dams. Salmonid loss due to predation will be overestimated using gut content analysis if some prey were dead or moribund when consumed. To examine this issue, field experiments were conducted in the Bonneville Dam tailrace (Columbia River) to compare rates of capture of live and dead juvenile salmonids by northern squawfish (Ptychocheilus oregonensis). Known numbers of coded-wire-tagged live and dead chinook salmon (O. tshawytscha) were released into the tailrace on six nights. Northern squawfish were collected after each release and their gut contents were examined for tags. When 50% of salmon released were dead, northern squawfish consumed 62% dead salmon. When 10% of salmon released were dead, comparable with dam passage mortality, 22% of the tags found in northern squawfish digestive tracts were from dead salmon. These results indicate that predator feeding behavior and prey condition are important considerations when estimating the impact of predation on a prey population.

  10. Polyphasic characterization of Aeromonas salmonicida isolates recovered from salmonid and non-salmonid fish

    USGS Publications Warehouse

    Diamanka, A.; Loch, T.P.; Cipriano, R.C.; Faisal, M.

    2013-01-01

    Michigan's fisheries rely primarily upon the hatchery propagation of salmonid fish for release in public waters. One limitation on the success of these efforts is the presence of bacterial pathogens, including Aeromonas salmonicida, the causative agent of furunculosis. This study was undertaken to determine the prevalence of A. salmonicida in Michigan fish, as well as to determine whether biochemical or gene sequence variability exists among Michigan isolates. A total of 2202 wild, feral and hatchery-propagated fish from Michigan were examined for the presence of A. salmonicida. The examined fish included Chinook salmon, Oncorhynchus tshawytscha (Walbaum), coho salmon, O. kisutcha (Walbaum), steelhead trout, O. mykiss (Walbaum), Atlantic salmon, Salmo salar L., brook trout, Salvelinus fontinalis (Mitchill), and yellow perch, Perca flavescens (Mitchill). Among these, 234 fish yielded a brown pigment-producing bacterium that was presumptively identified as A. salmonicida. Further phenotypic and phylogenetic analyses identified representative isolates as Aeromonas salmonicida subsp. salmonicida and revealed some genetic and biochemical variability. Logistic regression analyses showed that infection prevalence varied according to fish species/strain, year and gender, whereby Chinook salmon and females had the highest infection prevalence. Moreover, this pathogen was found in six fish species from eight sites, demonstrating its widespread nature within Michigan.

  11. Development and Application of a Salmonid EST Database and cDNA Microarray: Data Mining and Interspecific Hybridization Characteristics

    PubMed Central

    Rise, Matthew L.; von Schalburg, Kristian R.; Brown, Gordon D.; Mawer, Melanie A.; Devlin, Robert H.; Kuipers, Nathanael; Busby, Maura; Beetz-Sargent, Marianne; Alberto, Roberto; Gibbs, A. Ross; Hunt, Peter; Shukin, Robert; Zeznik, Jeffrey A.; Nelson, Colleen; Jones, Simon R.M.; Smailus, Duane E.; Jones, Steven J.M.; Schein, Jacqueline E.; Marra, Marco A.; Butterfield, Yaron S.N.; Stott, Jeff M.; Ng, Siemon H.S.; Davidson, William S.; Koop, Ben F.

    2004-01-01

    We report 80,388 ESTs from 23 Atlantic salmon (Salmo salar) cDNA libraries (61,819 ESTs), 6 rainbow trout (Oncorhynchus mykiss) cDNA libraries (14,544 ESTs), 2 chinook salmon (Oncorhynchus tshawytscha) cDNA libraries (1317 ESTs), 2 sockeye salmon (Oncorhynchus nerka) cDNA libraries (1243 ESTs), and 2 lake whitefish (Coregonus clupeaformis) cDNA libraries (1465 ESTs). The majority of these are 3′ sequences, allowing discrimination between paralogs arising from a recent genome duplication in the salmonid lineage. Sequence assembly reveals 28,710 different S. salar, 8981 O. mykiss, 1085 O. tshawytscha, 520 O. nerka, and 1176 C. clupeaformis putative transcripts. We annotate the submitted portion of our EST database by molecular function. Higher- and lower-molecular-weight fractions of libraries are shown to contain distinct gene sets, and higher rates of gene discovery are associated with higher-molecular weight libraries. Pyloric caecum library group annotations indicate this organ may function in redox control and as a barrier against systemic uptake of xenobiotics. A microarray is described, containing 7356 salmonid elements representing 3557 different cDNAs. Analyses of cross-species hybridizations to this cDNA microarray indicate that this resource may be used for studies involving all salmonids. PMID:14962987

  12. Invasion versus isolation: Trade-offs in managing native salmonids with barriers to upstream movement

    Treesearch

    Kurt D. Fausch; Bruce E. Rieman; Jason B. Dunham; Michael K. Young; Douglas P. Peterson

    2009-01-01

    Conservation biologists often face the trade-off that increasing connectivity in fragmented landscapes to reduce extinction risk of native species can foster invasion by non-native species that enter via the corridors created, which can then increase extinction risk. This dilemma is acute for stream fishes, especially native salmonids, because their populations are...

  13. Development of field-based models of suitable thermal regimes for interior Columbia Basin salmonids

    Treesearch

    Jason B. Dunham; Bruce Rieman; Gwynne Chandler

    2001-01-01

    This report describes results of research sponsored through an interagency agreement between the U.S. Forest Service Rocky Mountain Research Station and U.S. Environmental Protection Agency (Interagency Agreement #00-IA-11222014-521). The primary objectives of this research included 1) develop models relating occurrence of two threatened inland salmonid fishes to...

  14. Climate change impact on salmonid spawning in low-gradient streams in central Idaho, USA

    Treesearch

    Daniele Tonina; James A. McKean

    2010-01-01

    Climate change is often predicted to cause a significant perturbation to watershed hydrology. It has been generally associated with negative impacts on natural systems, especially in conjunction with conservation and protection of sensitive ecosystems. In the U.S., spawning habitats of threatened and endangered salmonid species are important areas that are potentially...

  15. [Principle directions for the creation and organization of the system of sanitary-epidemiological safety during the preparations for the XXII Olympic Winter Games and XI Paralympic Winter Games 2014 in Sochi].

    PubMed

    Onishchenko, G G; Bragina, I V; Ezhlova, E B; Demina, V P; Gorskiĭ, A A; Gus'kov, A S; Aksenova, O I; Ivanov, G E; Klindukhov, V P; Nikolaevich, P N; Grechanaia, T B; Kulichenko, A N; Maletskaia, O V; Manin, E A; Parkhomenko, V V; Kulichenko, O A

    2015-01-01

    The paper generalizes the experience of formation of protection system against biological threats and ensuring sanitary and epidemiological welfare during preparation for the XXII Olympic Winter Games and XI Paralympic Winter Games of 2014 in Sochi. The basic steps for creating this system, since 2007, participation and role of Rospotrebnadzor in this process are shown. The paper deals with such questions as the governmental and administrative structures with federal agencies interaction, development of a regulatory framework governing the safety system of the Olympic Games, development of algorithms of information exchange and management decisions, biological safety in developing infrastructure in Sochi.

  16. An evaluation of the toxicity of potassium chloride, active compound in the molluscicide potash, on salmonid fish and their forage base

    USGS Publications Warehouse

    Densmore, Christine L.; Iwanowicz, Luke R.; Henderson, Anne P.; Blazer, Vicki S.; Reed-Grimmett, Baileigh M.; Sanders, Lakyn R.

    2018-06-29

    Potash, with the active ingredient potassium chloride (KCl) is a chemical that is currently being evaluated for potential use as a molluscicide to combat invasive zebra mussels and quagga mussels in Western United States waters. Although data available for other freshwater fishes indicate that recommended treatment levels of potash as a molluscicide are sublethal, this has not been demonstrated for all salmonid species. The objectives of this study were to perform toxicity testing to determine the lethality of potassium chloride against selected species of salmonid fish (brook trout and Chinook salmon) and selected invertebrate forage, and to identify any potential adverse physiological impacts of KCl to these salmonids in water at treatment levels used for mollusk eradication. Minimal mortality (n=1 fish) was observed during 96-hour toxicity testing at KCl concentrations of 0 to 800 milligrams per liter (mg/L), indicating that the lethal concentration (LC50) values in these salmonid species were considerably higher than realistic molluscicide treatment concentrations. Sublethal effects were examined through evaluation of behavioral and morphological (histological) observation as well as specific blood chemistry parameters (electrolytes, osmolality, glucose, and cortisol). There was no strong evidence of significant physiological impairment among the two salmonid species due to KCl exposure. Whereas statistically significant differences in some parameters were observed in association with KCl treatments, it is unlikely that these differences indicate adverse biological impacts. Acute toxicity tests were conducted with invertebrate species at KCl exposure concentrations of 0–3,200 mg/L. Daphniid exposure trials resulted in differences in mortality among the test groups with higher mortality evident among the higher KCl exposure concentrations with a calculated LC50 value of 196 mg/L KCl for a 48-hour exposure. Crayfish exposed to higher concentrations of KCl at or

  17. Morphology and evolution of salmonid habitats in a recently deglaciated river basin, Washington state, USA.

    Treesearch

    L Benda; T.J. Beechie; R.C. Wissmar; A. Johnson

    1992-01-01

    Morphology and distribution of salmonid habitats were related to the geomorphology of a river basin at three spatial scales including reach (l02-103 m2), subbasin (2-26 km2), and the watershed (240 km2). Stream reaches on a young fluvial terrace (1700 yr...

  18. Facultative anadromy in salmonids: linking habitat, individual life history decisions, and population-level consequences

    Treesearch

    Steven F. Railsback; Bret C. Harvey; Jason L. White

    2014-01-01

    Modeling and management of facultative anadromous salmonids is complicated by their ability to select anadromous or resident life histories. Conventional theory for this behavior assumes individuals select the strategy offering highest expected reproductive success but does not predict how population-level consequences such as a stream’s smolt production emerge from...

  19. Potential Factors Affecting Survival Differ by Run-Timing and Location: Linear Mixed-Effects Models of Pacific Salmonids (Oncorhynchus spp.) in the Klamath River, California

    PubMed Central

    Quiñones, Rebecca M.; Holyoak, Marcel; Johnson, Michael L.; Moyle, Peter B.

    2014-01-01

    Understanding factors influencing survival of Pacific salmonids (Oncorhynchus spp.) is essential to species conservation, because drivers of mortality can vary over multiple spatial and temporal scales. Although recent studies have evaluated the effects of climate, habitat quality, or resource management (e.g., hatchery operations) on salmonid recruitment and survival, a failure to look at multiple factors simultaneously leaves open questions about the relative importance of different factors. We analyzed the relationship between ten factors and survival (1980–2007) of four populations of salmonids with distinct life histories from two adjacent watersheds (Salmon and Scott rivers) in the Klamath River basin, California. The factors were ocean abundance, ocean harvest, hatchery releases, hatchery returns, Pacific Decadal Oscillation, North Pacific Gyre Oscillation, El Niño Southern Oscillation, snow depth, flow, and watershed disturbance. Permutation tests and linear mixed-effects models tested effects of factors on survival of each taxon. Potential factors affecting survival differed among taxa and between locations. Fall Chinook salmon O. tshawytscha survival trends appeared to be driven partially or entirely by hatchery practices. Trends in three taxa (Salmon River spring Chinook salmon, Scott River fall Chinook salmon; Salmon River summer steelhead trout O. mykiss) were also likely driven by factors subject to climatic forcing (ocean abundance, summer flow). Our findings underscore the importance of multiple factors in simultaneously driving population trends in widespread species such as anadromous salmonids. They also show that the suite of factors may differ among different taxa in the same location as well as among populations of the same taxa in different watersheds. In the Klamath basin, hatchery practices need to be reevaluated to protect wild salmonids. PMID:24866173

  20. Assessing Juvenile Salmonid Passage Through Culverts: Field Research in Support of Protocol Development

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Williams, Greg D.; Evans, Nathan R.; Pearson, Walter H.

    2001-10-30

    The primary goal of our research this spring/ summer was to refine techniques and examine scenarios under which a standardized protocol could be applied to assess juvenile coho salmon (O. kisutch) passage through road culverts. Field evaluations focused on capture-mark- recapture methods that allowed analysis of fish movement patterns, estimates of culvert passability, and potential identification of cues inducing these movements. At this stage, 0+ age coho salmon fry 30 mm to 65 mm long (fork length) were the species and age class of interest. Ultimately, the protocol will provide rapid, statistically rigorous methods for trained personnel to perform standardizedmore » biological assessments of culvert passability to a number of juvenile salmon species. Questions to be addressed by the research include the following: ? Do hydraulic structures such as culverts restrict habitat for juvenile salmonids? ? How do existing culverts and retrofits perform relative to juvenile salmonid passage? ? Do some culvert characteristics and hydraulic conditions provide better passage than others? ? Does the culvert represent a barrier to certain size classes of fish? Recommendations addressed issues of study site selection, initial capture, marking, recapture/observations, and estimating movement.« less

  1. Virulence of Renibacterium salmoninarum to salmonids

    USGS Publications Warehouse

    Starliper, C.E.; Smith, D.R.; Shatzer, T.

    1997-01-01

    Virulence of Renibacterium salmoninarum isolates representing five origins was evaluated in eight salmonid hosts; four origins were of Lake Michigan and the fifth was of the Pacific Northwest. The species type strain, ATCC (American Type Culture Collection) 33209, was also included. Each isolate was grown in a kidney disease medium (KDM2) supplemented with 1 % ATCC 33209 culture metabolite; serial 10-fold dilutions were prepared, and groups of fish were challenged by intraperitoneal injection with 0.1 mL of each dilution. A 70-d observation period followed, and bacterial kidney disease (BKD) was diagnosed by the fluorescent antibody technique. Virulence of isolates was quantified as a dose lethal to 50% of fish (LD50) for each host–isolate challenge. In the first set of experiments, 23 isolates were used to challenge groups of brook trout Salvelinus fontinalis. The mean LD50 was 1.087 x 106 colony-forming units per milliliter (cfu/mL; SD = 2.022 x 106), and the LD50 values ranged from 8.457 x 106 to 2.227 x 104 cfu/mL. Analysis of variance to evaluate the effect of isolate origin on virulence in brook trout revealed no significant difference (F = 1.502; P = 0.243). Susceptibilities of the other salmonid hosts were evaluated by challenge with six isolates of R. salmoninarum representing each origin and the species type strain. For many of the host–isolate challenge combinations, time to death was highly dependent on the dilution (number of bacteria) injected. In general, the isolates MCO4M, B26, and A34 (all of Lake Michigan origin) tended to be more virulent. Also, LD50 values were dispersed throughout a wider range among the more susceptible hosts. Lake trout Salvelinus namaycush, rainbow trout Oncorhynchus mykiss, and brook trout were relatively resistant to challenge with the strains, whereas coho salmon O. kisutch, domestic Atlantic salmon Saltno salar, and chinook salmon O. tshawytscha were relatively susceptible. Another challenge evaluated the effect of

  2. Effects of woody debris on anadromous salmonid habitat, Prince of Wales Island, southeast Alaska

    Treesearch

    Thomas E. Lisle

    1986-01-01

    Abstract - The effects of woody debris on anadromous salmonid habitat in eight streams on Prince of Wales Island, southeast Alaska, were investigated by comparing low-gradient (1-9%) first- or second-order streams flowing through either spruce-hemlock forests or 6-10-year-old clearcuts, and by observing changes after debris was selectively removed from clear-cut...

  3. Predation by northern pikeminnow on juvenile salmonids in The Dalles Dam tailrace: field, laboratory, and habitat modeling studies (FY2000)

    USGS Publications Warehouse

    Petersen, James H.; Barfoot, Craig A.; Sheer, Mindi B.

    2001-01-01

    Predation by resident fish is known to be a substantial cause of juvenile salmonid mortality, especially in dam tailraces and outfall locations. Conditions in The Dalles Dam tailrace are unique compared to other projects on the Columbia or Snake rivers, having a complex basin with a series of downriver islands where predators are known to reside. In May-June of 1999, northern pikeminnow and smallmouth bass were sampled in the tailrace of The Dalles Dam during periods immediately following the release of PIT-tagged juvenile salmonids for survival studies. Over twice as many smallmouth bass (N = 101) were collected as northern pikeminnow (N = 40), but none of the predators had PIT tags within their gut. A laboratory study was conducted to estimate the time required for PIT tags in juvenile salmonids to be evacuated from the gut of northern pikeminnow after consuming a tagged preyfish. Evacuation rate was sensitive to temperature, with median evacuation time being 21 h at 18 oC and 30 h at 14 oC. These results suggest that field studies to estimate predator population sizes, feeding rates, or predation on specific release groups would require considerably more effort than we allocated during 1999.

  4. Froude Number is the Single Most Important Hydraulic Parameter for Salmonid Spawning Habitat.

    NASA Astrophysics Data System (ADS)

    Gillies, E.; Moir, H. J.

    2015-12-01

    Many gravel-bed rivers exhibit historic straightening or embanking, reducing river complexity and the available habitat for key species such as salmon. A defensible method for predicting salmonid spawning habitat is an important tool for anyone engaged in assessing a river restoration. Most empirical methods to predict spawning habitat use lookup tables of depth, velocity and substrate. However, natural site selection is different: salmon must pick a location where they can successfully build a redd, and where eggs have a sufficient survival rate. Also, using dimensional variables, such as depth and velocity, is problematic: spawning occurs in rivers of differing size, depth and velocity range. Non-dimensional variables have proven useful in other branches of fluid dynamics, and instream habitat is no different. Empirical river data has a high correlation between observed salmon redds and Froude number, without insight into why. Here we present a physics based model of spawning and bedform evolution, which shows that Froude number is indeed a rational choice for characterizing the bedform, substrate, and flow necessary for spawning. It is familiar for Froude to characterize surface waves, but Froude also characterizes longitudinal bedform in a mobile bed river. We postulate that these bedforms and their hydraulics perform two roles in salmonid spawning: allowing transport of clasts during redd building, and oxygenating eggs. We present an example of this Froude number and substrate based habitat characterization on a Scottish river for which we have detailed topography at several stages during river restoration and subsequent evolution of natural processes. We show changes to the channel Froude regime as a result of natural process and validate habitat predictions against redds observed during 2014 and 2015 spawning seasons, also relating this data to the Froude regime in other, nearby, rivers. We discuss the use of the Froude spectrum in providing an indicator of

  5. Predation on Chinook Salmon parr by hatchery salmonids and Fallfish in the Salmon River, New York

    USGS Publications Warehouse

    Johnson, James H.; Nack, Christopher C.; Chalupnicki, Marc; Abbett, Ross; McKenna, James E.

    2016-01-01

    Naturally reproduced Chinook Salmon Oncorhynchus tshawytscha contribute substantially to the fishery in Lake Ontario. The Salmon River, a Lake Ontario tributary in New York, produces the largest numbers of naturally spawned Chinook Salmon, with parr abundance in the river often exceeding 10 million. In the spring of each year, large numbers of hatchery salmonid yearlings—potential predators of Chinook Salmon parr—are released into the Salmon River by the New York State Department of Environmental Conservation. We sought to examine predation on Chinook Salmon parr in the Salmon River during May and June prior to out-migration. Over the 4 years examined (2009–2012), annual consumption of Chinook Salmon parr by hatchery-released yearling steelhead O. mykiss and Coho Salmon O. kisutch ranged from 1.5 to 3.3 million and from 0.4 to 2.1 million, respectively. In 2009, Fallfish Semotilus corporalis were estimated to consume 2.9 million Chinook Salmon parr. Predation was higher in May, when the average TL of Chinook Salmon parr was 44.5 mm, than in June. Fallfish were also important predators of naturally reproduced steelhead subyearlings, consuming an estimated 800,000 steelhead in 2009. Hatchery-released yearling salmonids consumed 13.8–15.3% of the Chinook Salmon parr that were estimated to be present in the Salmon River during 2010–2012. Earlier releases of hatchery salmonid yearlings could reduce the riverine consumption of Chinook Salmon parr by facilitating the out-migration of yearlings prior to Chinook Salmon emergence.

  6. Are block nets necessary? Movement of stream-dwelling salmonids in response to three common survey methods

    Treesearch

    James T. Peterson; Nolan P. Banish; Russell F. Thurow

    2005-01-01

    Fish movement during sampling may negatively bias sample data and population estimates. We evaluated the short-term movements of stream-dwelling salmonids by recapture of marked individuals during day and night snorkeling and backpack electrofishing. Bull trout Salvelinus confluentus and rainbow trout Oncorhynchus mykiss were...

  7. How well can captive breeding programs conserve biodiversity? A review of salmonids

    PubMed Central

    Fraser, Dylan J

    2008-01-01

    Captive breeding programs are increasingly being initiated to prevent the imminent extinction of endangered species and/or populations. But how well can they conserve genetic diversity and fitness, or re-establish self-sustaining populations in the wild? A review of these complex questions and related issues in salmonid fishes reveals several insights and uncertainties. Most programs can maintain genetic diversity within populations over several generations, but available research suggests the loss of fitness in captivity can be rapid, its magnitude probably increasing with the duration in captivity. Over the long-term, there is likely tremendous variation between (i) programs in their capacity to maintain genetic diversity and fitness, and (ii) species or even intraspecific life-history types in both the severity and manner of fitness-costs accrued. Encouragingly, many new theoretical and methodological approaches now exist for current and future programs to potentially reduce these effects. Nevertheless, an unavoidable trade-off exists between conserving genetic diversity and fitness in certain instances, such as when captive-bred individuals are temporarily released into the wild. Owing to several confounding factors, there is also currently little evidence that captive-bred lines of salmonids can or cannot be reintroduced as self-sustaining populations. Most notably, the root causes of salmonid declines have not been mitigated where captive breeding programs exist. Little research has also addressed under what conditions an increase in population abundance due to captive-rearing might offset fitness reductions induced in captivity. Finally, more empirical investigation is needed to evaluate the genetic/fitness benefits and risks associated with (i) maintaining captive broodstocks as either single or multiple populations within one or more facilities, (ii) utilizing cryopreservation or surrogate broodstock technologies, and (iii) adopting other alternatives to

  8. Research, Monitoring, and Evaluation of Avian Predation on Salmonid Smolts in the Lower and Mid-Columbia River, 2008 Draft Season Summary.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Roby, Daniel D.; Collis, Ken; Lyons, Donald E.

    2009-07-08

    This report describes investigations into predation by piscivorous colonial waterbirds on juvenile salmonids (Oncorhynchus spp.) from throughout the Columbia River basin during 2008. East Sand Island in the Columbia River estuary again supported the largest known breeding colony of Caspian terns (Hydroprogne caspia) in the world (approximately 10,700 breeding pairs) and the largest breeding colony of double-crested cormorants (Phalacrocorax auritus) in western North America (approximately 10,950 breeding pairs). The Caspian tern colony increased from 2007, but not significantly so, while the double-crested cormorant colony experienced a significant decline (20%) from 2007. Average cormorant nesting success in 2008, however, was downmore » only slightly from 2007, suggesting that food supply during the 2008 nesting season was not the principal cause of the decline in cormorant colony size. Total consumption of juvenile salmonids by East Sand Island Caspian terns in 2008 was approximately 6.7 million smolts (95% c.i. = 5.8-7.5 million). Caspian terns nesting on East Sand Island continued to rely primarily on marine forage fishes as a food supply. Based on smolt PIT tag recoveries on the East Sand Island Caspian tern colony, predation rates were highest on steelhead in 2008; minimum predation rates on steelhead smolts detected passing Bonneville Dam averaged 8.3% for wild smolts and 10.7% for hatchery-raised smolts. In 2007, total smolt consumption by East Sand Island double-crested cormorants was about 9.2 million juvenile salmonids (95% c.i. = 4.4-14.0 million), similar to or greater than that of East Sand Island Caspian terns during that year (5.5 million juvenile salmonids; 95% c.i. = 4.8-6.2 million). The numbers of smolt PIT tags recovered on the cormorant colony in 2008 were roughly proportional to the relative availability of PIT-tagged salmonids released in the Basin, suggesting that cormorant predation on salmonid smolts in the estuary was less selective

  9. Evaluation of an ion adsorption method to estimate intragravel flow velocity in salmonid spawning gravels

    Treesearch

    James L. Clayton; John G. King; Russell F. Thurow

    1996-01-01

    Intragravel water exchange provides oxygenated water, removes metabolic waste, and is an essential factor in salmonid embryo survival. Measurements of intragravel flow velocity have been suggested as an index of gravel quality and also as a useful predictor of fry emergence; however, proposed methods for measuring velocity in gravel are problematic. We evaluate an ion...

  10. Holding of juvenile salmonids for surgical implantation of electronic tags: a review and recommendations

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Oldenburg, Eric W.; Colotelo, Alison HA; Brown, Richard S.

    Many telemetry based studies require that fish be sampled from the wild and then held for some amount of time both prior to and after the implantation of a transmitter. However, the effects of such holding (or the lack thereof) are often overlooked. Pre-surgical holding often occurs to facilitate logistical needs of research projects and as an attempt to minimize negative physiological effects due to capture and handling stress. Further, post-surgical holding time and conditions greatly influence the physiological state of fish prior to being returned to the wild. This paper reviews pertinent studies pertaining to the effects of surgicalmore » holding on the behavior, physiology, and survival of fishes, with particular emphasis on juvenile salmonids. The effects of individual aspects of surgical holding such as stressors, time, holding conditions and water quality are also examined. Recommendations regarding certain aspects of surgical holding (e.g., holding duration) are offered with a goal of reducing bias related to the surgical process. Pre- and post-surgical holding times of 18–36 h are suggested as a general guideline for juvenile salmonids.« less

  11. Mathematical modeling in biological populations through branching processes. Application to salmonid populations.

    PubMed

    Molina, Manuel; Mota, Manuel; Ramos, Alfonso

    2015-01-01

    This work deals with mathematical modeling through branching processes. We consider sexually reproducing animal populations where, in each generation, the number of progenitor couples is determined in a non-predictable environment. By using a class of two-sex branching processes, we describe their demographic dynamics and provide several probabilistic and inferential contributions. They include results about the extinction of the population and the estimation of the offspring distribution and its main moments. We also present an application to salmonid populations.

  12. The role of density-dependent individual growth in the persistence of freshwater salmonid populations.

    PubMed

    Vincenzi, Simone; Crivelli, Alain J; Jesensek, Dusan; De Leo, Giulio A

    2008-06-01

    Theoretical and empirical models of populations dynamics have paid little attention to the implications of density-dependent individual growth on the persistence and regulation of small freshwater salmonid populations. We have therefore designed a study aimed at testing our hypothesis that density-dependent individual growth is a process that enhances population recovery and reduces extinction risk in salmonid populations in a variable environment subject to disturbance events. This hypothesis was tested in two newly introduced marble trout (Salmo marmoratus) populations living in Slovenian streams (Zakojska and Gorska) subject to severe autumn floods. We developed a discrete-time stochastic individual-based model of population dynamics for each population with demographic parameters and compensatory responses tightly calibrated on data from individually tagged marble trout. The occurrence of severe flood events causing population collapses was explicitly accounted for in the model. We used the model in a population viability analysis setting to estimate the quasi-extinction risk and demographic indexes of the two marble trout populations when individual growth was density-dependent. We ran a set of simulations in which the effect of floods on population abundance was explicitly accounted for and another set of simulations in which flood events were not included in the model. These simulation results were compared with those of scenarios in which individual growth was modelled with density-independent Von Bertalanffy growth curves. Our results show how density-dependent individual growth may confer remarkable resilience to marble trout populations in case of major flood events. The resilience to flood events shown by the simulation results can be explained by the increase in size-dependent fecundity as a consequence of the drop in population size after a severe flood, which allows the population to quickly recover to the pre-event conditions. Our results suggest

  13. Can biosecurity and local network properties predict pathogen species richness in the salmonid industry?

    PubMed Central

    More, Simon J.; Geoghegan, Fiona; McManus, Catherine; Hill, Ashley E.; Martínez-López, Beatriz

    2018-01-01

    Salmonid farming in Ireland is mostly organic, which implies limited disease treatment options. This highlights the importance of biosecurity for preventing the introduction and spread of infectious agents. Similarly, the effect of local network properties on infection spread processes has rarely been evaluated. In this paper, we characterized the biosecurity of salmonid farms in Ireland using a survey, and then developed a score for benchmarking the disease risk of salmonid farms. The usefulness and validity of this score, together with farm indegree (dichotomized as ≤ 1 or > 1), were assessed through generalized Poisson regression models, in which the modeled outcome was pathogen richness, defined here as the number of different diseases affecting a farm during a year. Seawater salmon (SW salmon) farms had the highest biosecurity scores with a median (interquartile range) of 82.3 (5.4), followed by freshwater salmon (FW salmon) with 75.2 (8.2), and freshwater trout (FW trout) farms with 74.8 (4.5). For FW salmon and trout farms, the top ranked model (in terms of leave-one-out information criteria, looic) was the null model (looic = 46.1). For SW salmon farms, the best ranking model was the full model with both predictors and their interaction (looic = 33.3). Farms with a higher biosecurity score were associated with lower pathogen richness, and farms with indegree > 1 (i.e. more than one fish supplier) were associated with increased pathogen richness. The effect of the interaction between these variables was also important, showing an antagonistic effect. This would indicate that biosecurity effectiveness is achieved through a broader perspective on the subject, which includes a minimization in the number of suppliers and hence in the possibilities for infection to enter a farm. The work presented here could be used to elaborate indicators of a farm’s disease risk based on its biosecurity score and indegree, to inform risk-based disease surveillance and

  14. Can biosecurity and local network properties predict pathogen species richness in the salmonid industry?

    PubMed

    Yatabe, Tadaishi; More, Simon J; Geoghegan, Fiona; McManus, Catherine; Hill, Ashley E; Martínez-López, Beatriz

    2018-01-01

    Salmonid farming in Ireland is mostly organic, which implies limited disease treatment options. This highlights the importance of biosecurity for preventing the introduction and spread of infectious agents. Similarly, the effect of local network properties on infection spread processes has rarely been evaluated. In this paper, we characterized the biosecurity of salmonid farms in Ireland using a survey, and then developed a score for benchmarking the disease risk of salmonid farms. The usefulness and validity of this score, together with farm indegree (dichotomized as ≤ 1 or > 1), were assessed through generalized Poisson regression models, in which the modeled outcome was pathogen richness, defined here as the number of different diseases affecting a farm during a year. Seawater salmon (SW salmon) farms had the highest biosecurity scores with a median (interquartile range) of 82.3 (5.4), followed by freshwater salmon (FW salmon) with 75.2 (8.2), and freshwater trout (FW trout) farms with 74.8 (4.5). For FW salmon and trout farms, the top ranked model (in terms of leave-one-out information criteria, looic) was the null model (looic = 46.1). For SW salmon farms, the best ranking model was the full model with both predictors and their interaction (looic = 33.3). Farms with a higher biosecurity score were associated with lower pathogen richness, and farms with indegree > 1 (i.e. more than one fish supplier) were associated with increased pathogen richness. The effect of the interaction between these variables was also important, showing an antagonistic effect. This would indicate that biosecurity effectiveness is achieved through a broader perspective on the subject, which includes a minimization in the number of suppliers and hence in the possibilities for infection to enter a farm. The work presented here could be used to elaborate indicators of a farm's disease risk based on its biosecurity score and indegree, to inform risk-based disease surveillance and control

  15. Watershed processes, fish habitat, and salmonid distribution in the Tonsina River (Copper River watershed), Alaska

    NASA Astrophysics Data System (ADS)

    Booth, D. B.; Ligon, F. K.; Sloat, M. R.; Amerson, B.; Ralph, S. C.

    2007-12-01

    The Copper River watershed is a critical resource for northeastern Pacific salmon, with annual escapements in the millions. The Tonsina River basin, a diverse 2100-km2 tributary to the Copper River that supports important salmonid populations, offers an opportunity to integrate watershed-scale channel network data with field reconnaissance of physical processes and observed distribution of salmonid species. Our long-term goals are to characterize habitats critical to different salmonid life stages, describe the geologic context and current geologic processes that support those habitats in key channel reaches, and predict their watershed-wide distribution. The overarching motivation for these goals is resource conservation, particularly in the face of increased human activity and long-term climate change. Channel geomorphology within the Tonsina River basin reflects inherited glacial topography. Combinations of drainage areas, slopes, channel confinement, and sediment-delivery processes are unique to this environment, giving rise to channel "types" that are recognizable but that do not occur in the same positions in the channel network as in nonglaciated landscapes. We also recognize certain channel forms providing fish habitat without analog in a nonglacial landscape, notably relict floodplain potholes from once-stranded and long-melted ice blocks. Salmonid species dominated different channel types within the watershed network. Sockeye salmon juveniles were abundant in the low-gradient, turbid mainstem; Chinook juveniles were also captured in the lower mainstem, with abundant evidence of spawning farther downstream. Coho juveniles were abundant in upper, relatively large tributaries, even those channels with cobble-boulder substrates and minimal woody debris that provide habitats more commonly utilized by Chinook in low-latitude systems. More detailed field sampling also revealed that patterns of species composition and abundance appeared related to small

  16. Virucidal activity of two Iodophors to salmonid viruses

    USGS Publications Warehouse

    Amend, Donald F.; Pietsch, John P.

    1972-01-01

    Wescodyne® and Betadine®, organic iodine complexes, were compared in vitro for virucidal activity against infectious hematopoietic necrosis (IHN), infectious pancreatic necrosis (IPN), and viral hemorrhagic septicemia (VHS) viruses. Both iodophors were about equally effective on all three viruses. Each iodophor completely destroyed IHN virus within 30 sec at 12 ppm iodine, and was not affected by water hardness. Virucidal activity, however, was reduced at pH levels above 8.0 and in the presence of organic matter. Wescodyne was also compared with seven disinfectants commonly used in fish hatcheries, for virucidal properties against IHN virus. Wescodyne and chlorine were the only disinfectants to completely destroy the virus. Either Wescodyne or Betadine would effectively destroy the salmonid viruses at less than 25 ppm iodine within 5 min in solutions near neutrality.

  17. Rate of disappearance of gas bubble trauma signs in juvenile salmonids

    USGS Publications Warehouse

    Hans, K.M.; Mesa, M.G.; Maule, A.G.

    1999-01-01

    To assess the rate of disappearance of gas bubble trauma (GBT) signs in juvenile salmonids, we exposed spring chinook salmon Oncorhynchus tshawytscha and steelhead O. mykiss to water containing high levels of dissolved gas supersaturation (DGS) for a time period sufficient to induce signs of GBT, reduced the DGS to minimal levels, and then sampled fish through time to document changes in severity of GBT. Because of the tendency of GBT signs to dissipate at different rates, we conducted trials focusing on emboli (bubbles) in the gill filaments and lateral line and separate trials that focused on bubbles in the external surfaces (fins, eyes, and opercula). Bubbles in gill filaments dissipated almost completely within 2 h after transfer of fish to water of nearly normal DGS (104%), whereas bubbles in the lateral line dissipated to negligible levels within 5 h. Bubbles on external surfaces were more persistent through time than they were in gill filaments and the lateral line. Although typically dissipating to low levels within 48 h, external bubbles sometimes remained for 4 d. Assuming a direct relation exists between easily observable signs and direct mortality, our results suggest that fish can recover quickly from the potentially lethal effects of DGS once they move from water with high DGS to water of almost normal gas saturation. These results should be of fundamental importance to fishery managers interpreting the results of monitoring for the severity and prevalence of GBT in juvenile salmonids in the Columbia River system and perhaps elsewhere.

  18. A simple model that identifies potential effects of sea-level rise on estuarine and estuary-ecotone habitat locations for salmonids in Oregon, USA.

    PubMed

    Flitcroft, Rebecca; Burnett, Kelly; Christiansen, Kelly

    2013-07-01

    Diadromous aquatic species that cross a diverse range of habitats (including marine, estuarine, and freshwater) face different effects of climate change in each environment. One such group of species is the anadromous Pacific salmon (Oncorhynchus spp.). Studies of the potential effects of climate change on salmonids have focused on both marine and freshwater environments. Access to a variety of estuarine habitat has been shown to enhance juvenile life-history diversity, thereby contributing to the resilience of many salmonid species. Our study is focused on the effect of sea-level rise on the availability, complexity, and distribution of estuarine, and low-freshwater habitat for Chinook salmon (Oncorhynchus tshawytscha), steelhead (anadromous O. mykiss), and coho salmon (O. kisutch) along the Oregon Coast under future climate change scenarios. Using LiDAR, we modeled the geomorphologies of five Oregon estuaries and estimated a contour associated with the current mean high tide. Contour intervals at 1- and 2-m increments above the current mean high tide were generated, and changes in the estuary morphology were assessed. Because our analysis relied on digital data, we compared three types of digital data in one estuary to assess the utility of different data sets in predicting the changes in estuary shape. For each salmonid species, changes in the amount and complexity of estuarine edge habitats varied by estuary. The simple modeling approach we applied can also be used to identify areas that may be most amenable to pre-emptive restoration actions to mitigate or enhance salmonid habitat under future climatic conditions.

  19. The VTAC Committee: developing guidance for an alternative regulatory pathway to the anadromous salmonid protection rules

    Treesearch

    Michael Liquori; Peter Cafferata; Kevin Boston; Richard Gienger; David Hope

    2012-01-01

    In recent decades, riparian protection standards have been guided by generalized prescriptive rules. With the passage of the Anadromous Salmonid Protection rules in 2009, the Board of Forestry and Fire Protection (Board) established a regulatory pathway that provides an alternative approach for riparian protection based on site-specific criteria (14 CCR § 916....

  20. Influence of fire on native and nonnative salmonid populations and habitat in a western Montana basin

    Treesearch

    Clint M Sestrich; Thomas E. McMahon; Michael K. Young

    2011-01-01

    Anticipated increases in the frequency and severity of wildfire may threaten the persistence of native salmonid populations in headwater streams in western North America. This study used extensive pre- and postfire data to assess whether wildfire leads to hypothesized declines in native westslope cutthroat trout Oncorhynchus clarkii lewisi and bull trout Salvelinus...

  1. Carcass addition does not enhance juvenile salmonid biomass, growth, or retention in six Northwestern California streams.

    Treesearch

    Bret Harvey; Margaret A. Wilzbach

    2010-01-01

    Fisheries managers commonly consider the addition of salmon carcasses when seeking to enhance salmonid populations. However, the range of environmental conditions under which the technique is effective remains poorly defined. We addressed this issue by measuring the effects of wintertime addition of salmon carcasses on the biomass, growth, and retention of juvenile...

  2. A riverscape perspective of Pacific salmonids and aquatic habitats prior to large-scale dam removal in the Elwha River, Washington, USA

    USGS Publications Warehouse

    Brenkman, S.J.; Duda, J.J.; Torgersen, C.E.; Welty, E.; Pess, G.R.; Peters, R.; McHenry, M.L.

    2012-01-01

     Dam removal has been increasingly proposed as a river restoration technique. In 2011, two large hydroelectric dams will be removed from Washington State’s Elwha River. Ten anadromous fish populations are expected to recolonise historical habitats after dam removal. A key to understanding watershed recolonisation is the collection of spatially continuous information on fish and aquatic habitats. A riverscape approach with an emphasis on biological data has rarely been applied in mid-sized, wilderness rivers, particularly in consecutive years prior to dam removal. Concurrent snorkel and habitat surveys were conducted from the headwaters to the mouth (rkm 65–0) of the Elwha River in 2007 and 2008. This riverscape approach characterised the spatial extent, assemblage structure and patterns of relative density of Pacific salmonids. The presence of dams influenced the longitudinal patterns of fish assemblages, and species richness was the highest downstream of the dams, where anadromous salmonids still have access. The percent composition of salmonids was similar in both years for rainbow trout, Oncorhynchus mykiss (Walbaum), coastal cutthroat trout, Oncorhynchus clarkii clarkii (Richardson) (89%; 88%), Chinook salmon, Oncorhynchus tshawytscha (Walbaum) (8%; 9%), and bull trout, Salvelinus confluentus (Suckley) (3% in both years). Spatial patterns of abundance for rainbow and cutthroat trout (r = 0.76) and bull trout (r = 0.70) were also consistent between years. Multivariate and univariate methods detected differences in habitat structure along the river profile caused by natural and anthropogenic factors. The riverscape view highlighted species-specific biological hotspots and revealed that 60–69% of federally threatened bull trout occurred near or below the dams. Spatially continuous surveys will be vital in evaluating the effectiveness of upcoming dam removal projects at restoring anadromous salmonids.

  3. Predicting the Total Abundance of Resident Salmonids within the Willamette River Basin, Oregon - a Macroecological Modeling Approach

    EPA Science Inventory

    I present a simple, macroecological model of fish abundance that was used to estimate the total number of non-migratory salmonids within the Willamette River Basin (western Oregon). The model begins with empirical point estimates of net primary production (NPP in g C/m2) in fore...

  4. Impact of beaver dams on abundance and distribution of anadromous salmonids in two lowland streams in Lithuania.

    PubMed

    Virbickas, Tomas; Stakėnas, Saulius; Steponėnas, Andrius

    2015-01-01

    European beaver dams impeded movements of anadromous salmonids as it was established by fishing survey, fish tagging and redd counts in two lowland streams in Lithuania. Significant differences in abundancies of other litophilic fish species and evenness of representation by species in the community were detected upstream and downstream of the beaver dams. Sea trout parr marked with RFID tags passed through several successive beaver dams in upstream direction, but no tagged fish were detected above the uppermost dam. Increase in abundances of salmonid parr in the stream between the beaver dams and decrease below the dams were recorded in November, at the time of spawning of Atlantic salmon and sea trout, but no significant changes were detected in the sections upstream of the dams. After construction of several additional beaver dams in the downstream sections of the studied streams, abundance of Atlantic salmon parr downstream of the dams decreased considerably in comparison with that estimated before construction.

  5. Juvenile salmonid use of freshwater emergent wetlands in the floodplain and its implications for conservation management

    USGS Publications Warehouse

    Henning, Julie A.; Gresswell, Robert E.; Fleming, Ian A.

    2006-01-01

    A recent trend of enhancing freshwater emergent wetlands for waterfowl and other wildlife has raised concern about the effects of such measures on juvenile salmonids. We undertook this study to quantify the degree and extent of juvenile Pacific salmon Oncorhynchus spp. utilization of enhanced and unenhanced emergent wetlands within the floodplain of the lower Chehalis River, Washington, and to determine the fate of the salmon using them. Enhanced emergent wetlands contained water control structures that provided an outlet for fish emigration and a longer hydroperiod for rearing than unenhanced wetlands. Age-0 and age-1 coho salmon O. kisutch were the most common salmonid at all sites, enhanced wetlands having significantly higher age-1 abundance than unenhanced wetlands that were a similar distance from the main-stem river. Yearling coho salmon benefited from rearing in two enhanced wetland habitats, where their specific growth rate and minimum estimates of survival (1.43%/d by weight and 30%; 1.37%/d and 57%) were comparable to those in other side-channel rearing studies. Dissolved oxygen concentrations decreased in emergent wetlands throughout the season and approached the limits lethal to juvenile salmon by May or June each year. Emigration patterns suggested that age-0 and age-1 coho salmon emigrated as habitat conditions declined. This observation was further supported by the results of an experimental release of coho salmon. Survival of fish utilizing emergent wetlands was dependent on movement to the river before water quality decreased or stranding occurred from wetland desiccation. Thus, our results suggest that enhancing freshwater wetlands via water control structures can benefit juvenile salmonids, at least in the short term, by providing conditions for greater growth, survival, and emigration.

  6. Non-native salmonids affect amphibian occupancy at multiple spatial scales

    USGS Publications Warehouse

    Pilliod, David S.; Hossack, Blake R.; Bahls, Peter F.; Bull, Evelyn L.; Corn, Paul Stephen; Hokit, Grant; Maxell, Bryce A.; Munger, James C.; Wyrick, Aimee

    2010-01-01

    Aim The introduction of non-native species into aquatic environments has been linked with local extinctions and altered distributions of native species. We investigated the effect of non-native salmonids on the occupancy of two native amphibians, the long-toed salamander (Ambystoma macrodactylum) and Columbia spotted frog (Rana luteiventris), across three spatial scales: water bodies, small catchments and large catchments. Location Mountain lakes at ≥ 1500 m elevation were surveyed across the northern Rocky Mountains, USA. Methods We surveyed 2267 water bodies for amphibian occupancy (based on evidence of reproduction) and fish presence between 1986 and 2002 and modelled the probability of amphibian occupancy at each spatial scale in relation to habitat availability and quality and fish presence. Results After accounting for habitat features, we estimated that A. macrodactylum was 2.3 times more likely to breed in fishless water bodies than in water bodies with fish. Ambystoma macrodactylum also was more likely to occupy small catchments where none of the water bodies contained fish than in catchments where at least one water body contained fish. However, the probability of salamander occupancy in small catchments was also influenced by habitat availability (i.e. the number of water bodies within a catchment) and suitability of remaining fishless water bodies. We found no relationship between fish presence and salamander occupancy at the large-catchment scale, probably because of increased habitat availability. In contrast to A. macrodactylum, we found no relationship between fish presence and R. luteiventris occupancy at any scale. Main conclusions Our results suggest that the negative effects of non-native salmonids can extend beyond the boundaries of individual water bodies and increase A. macrodactylum extinction risk at landscape scales. We suspect that niche overlap between non-native fish and A. macrodactylum at higher elevations in the northern Rocky

  7. Cumulative effects of logging road sediment on salmonid populations in the Clearwater River, Jefferson County, Washington

    Treesearch

    C. J. Cederholm; L. M. Reid; E. O. Salo

    1981-01-01

    Abstract - The nature of sediment production from logging roads and the effect of the resulting sediment on salmonid spawning success in the Clearwater River drainage have been studied for eight years. The study includes intensive and extensive analyses of field situations, supplemented by several controlled experiments. It was found that significant amounts (15-25...

  8. Spatial and temporal heterogeneity of infectious hematopoietic necrosis virus in Pacific Northwest salmonids

    USGS Publications Warehouse

    Breyta, Rachel; Black, Allison; Kaufman, John; Kurath, Gael

    2016-01-01

    The aquatic rhaboviral pathogen infectious hematopoietic necrosis virus (IHNV) causes acute disease in juvenile fish of a number of populations of Pacific salmonid species. Heavily managed in both marine and freshwater environments, these fish species are cultured during the juvenile stage in freshwater conservation hatcheries, where IHNV is one of the top three infectious diseases that cause serious morbidity and mortality. Therefore, a comprehensive study of viral genetic surveillance data representing 2590 field isolates collected between 1958 and 2014 was conducted to determine the spatial and temporal patterns of IHNV in the Pacific Northwest of the contiguous United States. Prevalence of infection varied over time, fluctuating over a rough 5–7 year cycle. The genetic analysis revealed numerous subgroups of IHNV, each of which exhibited spatial heterogeneity. Within all subgroups, dominant genetic types were apparent, though the temporal patterns of emergence of these types varied among subgroups. Finally, the affinity or fidelity of subgroups to specific host species also varied, where UC subgroup viruses exhibited a more generalist profile and all other subgroups exhibited a specialist profile. These complex patterns are likely synergistically driven by numerous ecological, pathobiological, and anthropogenic factors. Since only a few anthropogenic factors are candidates for managed intervention aimed at improving the health of threatened or endangered salmonid fish populations, determining the relative impact of these factors is a high priority for future studies.

  9. Magnitude and Dynamics of Predation on Juvenile Salmonids in Columbia and Snake River Reservoirs, Annual Report of Research, 1989-1990.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Petersen, J.H.

    1990-07-01

    Three aspects of predation upon juvenile salmonids in the Columbia River are addressed in this report: (1) Indexing predator consumption. During 1989--1990, two indices of northern squawfish consumption upon juvenile salmonids were developed for use throughout the Columbia River Basin. The direct Consumption Index (CI) is based upon the concept of meal turnover time and takes into account number of salmonids, temperature, total gut content weight and predator weight. A Bioenergetics Index (BI) for consumption indexing was also developed to complement the direct CI. In the BI, growth, consumption, excretion/evacuation and respiration processes are modeled to predict the consumption requiredmore » to produce an observed growth increment. (2) Studies on predator-smolt dynamics. Northern squawfish consumption data were collected in the McNary Dam tailrace during nine days in July 1988 to improve our understanding of the predator-smolt functional response. (3) Selective predation by northern squawfish. Laboratory and field protocols were developed to evaluate northern squawfish selection and prey vulnerability. Results from laboratory studies suggest that northern squawfish prefer dead over live prey and that descaled prey may be more vulnerable to predation than non-descaled prey. Stressed and unstressed prey were consumed in equal proportions when predation occurred for 6 or 24 h. Physiological and behavioral effects of stress on juvenile salmon are presented. 100 refs., 13 figs., 12 tabs.« less

  10. Habitat associations of age-0 cutthroat trout in a spring stream improved for adult salmonids

    USGS Publications Warehouse

    Hubert, W.A.; Joyce, M.P.

    2005-01-01

    Native cutthroat trout (Oncorhynchus clarki) in the Snake River watershed use streams formed by large springs for spawning and nursery habitat. Several spring streams have been modified to enhance abundance of adult salmonids, but the habitat associations of age-0 cutthroat trout in these systems are undescribed. We assessed the frequency of collection of age-0 cutthroat trout in riffles, riffle margins, pool margins, and backwaters from late June to the middle of August 2000 in a spring stream with such modifications. The proportion of sites in which age-0 cutthroat trout were collected increased up to the middle of July and then decreased. We found substantially lower frequencies of collection of age-0 cutthroat trout in riffles compared to the three stream-margin habitat types. Age-0 cutthroat trout appeared to select shallow, low-velocity, stream-margin habitat with cover that provided protection from piscivorous adult salmonids and avian predators. Our observations suggest that modification of spring streams for production of cutthroat trout should include efforts to manage stream margins so they provide cover in the form of aquatic macrophytes or overhanging vegetation for age-0 fish.

  11. Relationship of otolith strontium-to-calcium ratios and salinity: Experimental validation for juvenile salmonids

    USGS Publications Warehouse

    Zimmerman, C.E.

    2005-01-01

    Analysis of otolith strontium (Sr) or strontium-to-calcium (Sr:Ca) ratios provides a powerful tool to reconstruct the chronology of migration among salinity environments for diadromous salmonids. Although use of this method has been validated by examination of known individuals and translocation experiments, it has never been validated under controlled experimental conditions. In this study, incorporation of otolith Sr was tested across a range of salinities and resulting levels of ambient Sr and Ca concentrations in juvenile chinook salmon (Oncorhynchus tshawytscha), coho salmon (Oncorhynchus kisutch), sockeye salmon (Oncorhynchus nerka), rainbow trout (Oncorhynchus rnykiss), and Arctic char (Salvelinus alpinus). Experimental water was mixed, using stream water and seawater as end members, to create experimental salinities of 0.1, 6.3, 12.7, 18.6, 25.5, and 33.0 psu. Otolith Sr and Sr:Ca ratios were significantly related to salinity for all species (r2 range: 0.80-0.91) but provide only enough predictive resolution to discriminate among fresh water, brackish water, and saltwater residency. These results validate the use of otolith Sr:Ca ratios to broadly discriminate salinity histories encountered by salmonids but highlight the need for further research concerning the influence of osmoregulation and physiological changes associated with smoking on otolith microchemistry.

  12. Effects of sediment transport on survival of salmonid embryos in a natural stream: A simulation approach

    Treesearch

    Thomas E. Lisle; Jack Lewis

    1992-01-01

    A model is presented that simulates the effects of streamflow and sediment transport on survival of salmonid embryos incubating in spawning gravels in a natural channel. Components of the model include a 6-yr streamflow record, an empirical bed load-transport function, a relation between transport and infiltration of sandy bedload into a gravel bed, effects of fine-...

  13. Salmonid Communities in the South Fork of Caspar Creek, 1967 to 1969 and 1993 to 2003

    Treesearch

    Bradley E. Valentine; Richard A. Macedo; Tracie Hughes

    2007-01-01

    Demand for wood products and advances in logging technology post-World War II resulted in timber harvesting that extensively modified streams on the North Coast of California. To assess the resulting impacts to salmonid populations, the Department of Fish and Game conducted studies at widely spaced sites throughout the redwood region during the 1960s. In order to...

  14. Cardiorespiratory performance during prolonged swimming tests with salmonids: a perspective on temperature effects and potential analytical pitfalls.

    PubMed

    Farrell, A P

    2007-11-29

    A prolonged swimming trial is the most common approach in studying steady-state changes in oxygen uptake, cardiac output and tissue oxygen extraction as a function of swimming speed in salmonids. The data generated by these sorts of studies are used here to support the idea that a maximum oxygen uptake is reached during a critical swimming speed test. Maximum oxygen uptake has a temperature optimum. Potential explanations are advanced to explain why maximum aerobic performance falls off at high temperature. The valuable information provided by critical swimming tests can be confounded by non-steady-state swimming behaviours, which typically occur with increasing frequency as salmonids approach fatigue. Two major concerns are noted. Foremost, measurements of oxygen uptake during swimming can considerably underestimate the true cost of transport near critical swimming speed, apparently in a temperature-dependent manner. Second, based on a comparison with voluntary swimming ascents in a raceway, forced swimming trials in a swim tunnel respirometer may underestimate critical swimming speed, possibly because fish in a swim tunnel respirometer are unable to sustain a ground speed.

  15. Effects of riparian canopy opening and salmon carcass addition on the abundance and growth of resident salmonids

    Treesearch

    Margaret A. Wilzbach; Bret C. Harvey; Jason L. White; Rodney J. Nakamoto

    2005-01-01

    We studied the concurrent effects of riparian canopy opening and salmon carcass addition on salmonid biomass, density and growth rates in small streams over 2 years. In each of six streams in the Smith and Klamath River basins in northern California, red alder (Alnus rubra) and other hardwoods were removed along both banks of a 100-m reach to...

  16. Acoustic Imaging Evaluation of Juvenile Salmonid Behavior in the Immediate Forebay of the Water Temperature Control Tower at Cougar Dam, 2010

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Khan, Fenton; Johnson, Gary E.; Royer, Ida M.

    This report presents the results of an evaluation of juvenile Chinook salmonid (Oncorhynchus tshawytscha) behavior in the immediate forebay of the Water Temperature Control (WTC) tower at Cougar Dam in 2010. The study was conducted by the Pacific Northwest National Laboratory for the U.S. Army Corps of Engineers. The overall goal of the study was to characterize juvenile salmonid behavior and movement patterns in the immediate forebay of the WTC tower for fisheries resource managers to use to make decisions on bioengineering designs for long-term structures and/or operations to facilitate safe downstream passage for juvenile salmonids. We collected acoustic imagingmore » (Dual-Frequency Identification Sonar; DIDSON) data from February 1, 2010 through January 31, 2011 to evaluate juvenile salmonid behavior year-round in the immediate forebay surface layer of the WTC tower (within 20 m, depth 0-5 m). From October 28, 2010 through January 31, 2011 a BlueView acoustic camera was also deployed in an attempt to determine its usefulness for future studies as well as augment the DIDSON data. For the DIDSON data, we processed a total of 35 separate 24-h periods systematically covering every other week in the 12-month study. Two different 24-hour periods were processed for the BlueView data for the feasibility study. Juvenile salmonids were present in the immediate forebay of the WTC tower throughout 2010. The juvenile salmonid abundance index was low in the spring (<200 fish per sample-day), began increasing in late April and peaked in mid-May. Fish abundance index began decreasing in early June and remained low in the summer months. Fish abundance increased again in the fall, starting in October, and peaked on November 8-9. A second peak occurred on December 22. Afterwards, abundance was low for the rest of the study (through January 2011). Average fish length for juvenile salmonids during early spring 2010 was 214 {+-} 86 mm (standard deviation). From May through early

  17. Juvenile anadromous salmonid production in upper Columbia River side channels with different levels of hydrological connection

    USGS Publications Warehouse

    Martens, Kyle D.; Connolly, Patrick J.

    2014-01-01

    We examined the contribution of three types of side channels based on their hydrologic connectivity (seasonally disconnected, partially connected, and connected) to production of juvenile anadromous salmonids. Juvenile steelhead Oncorhynchus mykiss and Chinook Salmon O. tshawytscha were found in all three of these side channel types and in each year of the study. Upon connection with the main stem at high flows, the seasonally disconnected side channels experienced an emptying out of the previous year's fish while filling with young-of-year fish during the 2- to 4-month period of hydrologic connection. There were no differences between the densities of juvenile steelhead and Chinook Salmon and the rate of smolts produced among the three types of side channels. Recently reintroduced Coho Salmon O. kisutch had sporadic presence and abundance in partially and connected side channels, but the smolt production rate was over two times that of steelhead and Chinook Salmon in seasonally disconnected side channels. Within seasonally disconnected side channels, young-of-year salmonids in deep pools (≥100 cm) had greater survival than those in shallow pools (<100 cm). Densities of juvenile steelhead in all side channel types were similar to those in tributaries and were higher than in main-stem lateral margins. Juvenile Chinook Salmon densities were higher in side channels than in both tributary and main-stem lateral margins. Our results suggest that improving quality of pool habitat within seasonally disconnected side channels can result in improved survival for juvenile anadromous salmonids during the period of disconnection. Habitat improvement in these seasonally disconnected side channels should be recognized as a worthy restoration strategy, especially when full connectivity of side channels may not be a feasible target (e.g., through lack of water availability) or when full connectivity may present too high a risk (e.g., flooding, stream capture, bank

  18. Influence of forest and rangeland management on anadromous fish habitat in Western North America: habitat requirements of anadromous salmonids.

    Treesearch

    D.W. Reiser; T.C. Bjornn

    1979-01-01

    Habitat requirements of anadromous and some resident salmonid fishes have been described for various life stages, including upstream migration of adults, spawning, incubation, and juvenile rearing. Factors important in the migration of adults are water temperature, minimum water depth, maximum water velocity, turbidity, dissolved oxygen, and...

  19. Basin-scale availability of salmonid spawning gravel as influenced by channel type and hydraulic roughness in mountain catchments.

    Treesearch

    John M. Buffington; David R. Montgomery; Harvey M. Greenberg

    2004-01-01

    A general framework is presented for examining the effects of channel type and associated hydraulic roughness on salmonid spawning-gravel availability in mountain catchments. Digital elevation models are coupled with grain-size predictions to provide basin-scale assessments of the potential extent and spatial pattern of spawning gravels. To demonstrate both the model...

  20. [FEATURES OF THE ORGANIZATION OF SANITARY-EPIDEMIOLOGICAL SURVEILLANCE DURING THE PERIOD OF PREPARATION AND HOSTING OF THE XXII OLYMPIC WINTER GAMES AND XI PARALYMPIC WINTER GAMES IN THE RESORT CITY OF SOCHI IN 2014].

    PubMed

    Onishchenko, G G; Popova, A Iu; Kuzkin, B P; Guskova, A S; Ivanov, G E; Pakskina, N D; Klindukhov, V P; Nikolaevich, P N; Grechanaia, T V; Balaeva, M I; Biriukov, V A; Bozhko, I I; Tesheva, S Ch; Daragan, Iu G; Parkhomenko, V V; Rafeenko, G K; Kulichenko, A N; Manin, E A; Maletskaia, O V; Vasilenko, N F; Efremenko, D V; Orobeĭ, V G; Eldinova, V E; Pilikova, O M; Malaĭ, V I; Iunicheva, Iu V

    2015-01-01

    In the paper there are presented the basic principles of the organization of activities for the assurance ofthe sanitary- epidemiological welfare in the period ofpreparation and hosting of the XXII Olympic Winter Games and XI Paralympic Winter Games of 2014 in the Resort City of Sochi. There are considered features of the organization ofepidemiological surveillance in the pre-Olympic period, the period of the games and the state of the morbidity rate in the region after the Olympics. There are presented data on certain directions of the work of organs and institutions of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare on the disease control of the event.

  1. Natural Hazards Observer. Volume XXII, Number 3. January 1998.

    DTIC Science & Technology

    1998-01-01

    National Science Foundation , Federal Emergency Management Agency, National Oceanic and Atmospheric Administration, U.S. Geological Survey, U.S. Army Corps of Engineers, U.S. Forest Service, Environmental Protection Agency, U.S. Department of Transportation, National Aeronautics and Space Administration, and the Institute for Business and Home Safety. The Natural Hazards Observer is published

  2. Influence of riparian canopy on macroinvertebrate composition and food habits of juvenile salmonids in several Oregon streams.

    Treesearch

    William R. Meehan

    1996-01-01

    The community composition of macroinvertebrates and the feeding habits of juvenile salmonids were studied in eight Oregon streams. Benthic, drift, sticky trap, and water trap samples were taken over a 3-year period, along with stomach samples of the fish. Samples were taken in stream reaches with and without riparian canopy. Both main effects—fish diet versus...

  3. Trophic feasibility of reintroducing anadromous salmonids in three reservoirs on the north fork Lewis River, Washington: Prey supply and consumption demand of resident fishes

    USGS Publications Warehouse

    Sorel, Mark H.; Hansen, Adam G.; Connelly, Kristin A.; Beauchamp, David A.

    2016-01-01

    The reintroduction of anadromous salmonids in reservoirs is being proposed with increasing frequency, requiring baseline studies to evaluate feasibility and estimate the capacity of reservoir food webs to support reintroduced populations. Using three reservoirs on the north fork Lewis River as a case study, we demonstrate a method to determine juvenile salmonid smolt rearing capacities for lakes and reservoirs. To determine if the Lewis River reservoirs can support reintroduced populations of juvenile stream-type Chinook Salmon Oncorhynchus tshawytscha, we evaluated the monthly production of daphniaDaphnia spp. (the primary zooplankton consumed by resident salmonids in the system) and used bioenergetics to model the consumption demand of resident fishes in each reservoir. To estimate the surplus of Daphnia prey available for reintroduced salmonids, we assumed a maximum sustainable exploitation rate and accounted for the consumption demand of resident fishes. The number of smolts that could have been supported was estimated by dividing any surplus Daphnia production by the simulated consumption demand of an individual Chinook Salmon fry rearing in the reservoir to successful smolt size. In all three reservoirs, densities of Daphnia were highest in the epilimnion, but warm epilimnetic temperatures and the vertical distribution of planktivores suggested that access to abundant epilimnetic prey was limited. By comparing accessible prey supply and demand on a monthly basis, we were able to identify potential prey supply bottlenecks that could limit smolt production and growth. These results demonstrate that a bioenergetics approach can be a valuable method of examining constraints on lake and reservoir rearing capacity, such as thermal structure and temporal food supply. This method enables numerical estimation of rearing capacity, which is a useful metric for managers evaluating the feasibility of reintroducing Pacific salmon Oncorhynchus spp. in lentic systems.

  4. Salmonid genomes have a remarkably expanded akirin family, coexpressed with genes from conserved pathways governing skeletal muscle growth and catabolism.

    PubMed

    Macqueen, Daniel J; Kristjánsson, Bjarni K; Johnston, Ian A

    2010-06-01

    Metazoan akirin genes regulate innate immunity, myogenesis, and carcinogenesis. Invertebrates typically have one family member, while most tetrapod and teleost vertebrates have one to three. We demonstrate an expanded repertoire of eight family members in genomes of four salmonid fishes, owing to paralog preservation after three tetraploidization events. Retention of paralogs secondarily lost in other teleosts may be related to functional diversification and posttranslational regulation. We hypothesized that salmonid akirins would be transcriptionally regulated in fast-twitch skeletal muscle during activation of conserved pathways governing catabolism and growth. The in vivo nutritional state of Arctic charr (Salvelinus alpinus L.) was experimentally manipulated, and transcript levels for akirin family members and 26 other genes were measured by quantitative real-time PCR (qPCR), allowing the establishment of a similarity network of expression profiles. In fasted muscle, a class of akirins was upregulated, with one family member showing high coexpression with catabolic genes coding the NF-kappaB p65 subunit, E2 ubiquitin-conjugating enzymes, E3 ubiquitin ligases, and IGF-I receptors. Another class of akirin was upregulated with subsequent feeding, coexpressed with 14-3-3 protein genes. There was no similarity between expression profiles of akirins with IGF hormones or binding protein genes. The level of phylogenetic relatedness of akirin family members was not a strong predictor of transcriptional responses to nutritional state, or differences in transcript abundance levels, indicating a complex pattern of regulatory evolution. The salmonid akirins epitomize the complexity linking the genome to physiological phenotypes of vertebrates with a history of tetraploidization.

  5. Determine the Influence of Time Held in “Knockdown” Anesthesia on Survival and Stress of Surgically Implanted Juvenile Salmonids

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Woodley, Christa M.; Wagner, Katie A.; Knox, Kasey M.

    2012-01-31

    The Juvenile Salmon Acoustic Telemetry System (JSATS) was developed for the U.S. Army Corp of Engineers Portland District (USACE) to address questions related to survival and performance measures of juvenile salmonids as they pass through the Federal Columbia River Power System (FCRPS). Researchers using JSATS acoustic transmitters (ATs) were tasked with standardizing the surgical implantation procedure to ensure that the stressors of handling and surgery on salmonids were consistent and less likely to cause effects of tagging in survival studies. Researchers questioned whether the exposure time in 'knockdown' anesthesia (or induction) to prepare fish for surgery could influence the survivalmore » of study fish (CBSPSC 2011). Currently, fish are held in knockdown anesthesia after they reach Stage 4 anesthesia until the completion of the surgical implantation of a transmitter, varies from 5 to 15 minutes for studies conducted in the Columbia Basin. The Columbia Basin Surgical Protocol Steering Committee (CBSPSC ) expressed concern that its currently recommended 10-minute maximum time limit during which fish are held in anesthetic - tricaine methanesulfonate (MS-222, 80 mg L-1 water) - could increase behavioral and physiological costs, and/or decrease survival of outmigrating juvenile salmonids. In addition, the variability in the time fish are held at Stage 4 could affect the data intended for direct comparison of fish within or among survival studies. Under the current recommended protocol, if fish exceed the 10-minute time limit, they are to be released without surgical implantation, thereby increasing the number of fish handled and endangered species 'take' at the bypass systems for FCRPS survival studies.« less

  6. Pathways of Barotrauma in Juvenile Salmonids Exposed to Simulated Hydroturbine Passage: Boyle’s Law vs. Henry’s Law

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Brown, Richard S.; Pflugrath, Brett D.; Colotelo, Alison HA

    2012-06-01

    On their seaward migration, juvenile salmonids commonly pass hydroelectric dams. Fish passing by the turbine blade may experience rapid decompression, the severity of which can be highly variable and may result in a number of barotraumas. The mechanisms of these injuries can be due to expansion of existing bubbles or gases coming out of solution; governed by Boyle’s Law and Henry’s Law, respectively. This paper combines re-analysis of published data with new experiments to gain a better understanding of the mechanisms of injury and mortality for fish experiencing rapid decompression associated with hydroturbine passage. From these data it appears thatmore » the majority of decompression related injuries are due to the expansion of existing bubbles in the fish, particularly the expansion and rupture of the swim bladder. This information is particularly useful for fisheries managers and turbine manufacturers, demonstrating that reducing the rate of swim bladder ruptures by reducing the frequency of occurrence and severity of rapid decompression during hydroturbine passage could reduce the rates of injury and mortality for hydroturbine passed juvenile salmonids.« less

  7. Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis

    USGS Publications Warehouse

    Chase, D.M.; Elliott, D.G.; Pascho, R.J.

    2006-01-01

    Renibacterium salmoninarum is an important salmonid pathogen that is difficult to culture. We developed and assessed a real-time, quantitative, polymerase chain reaction (qPCR) assay for the detection and enumeration of R. salmoninarum. The qPCR is based on TaqMan technology and amplifies a 69-base pair (bp) region of the gene encoding the major soluble antigen (MSA) of R. salmoninarum. The qPCR assay consistently detected as few as 5 R. salmoninarum cells per reaction in kidney tissue. The specificity of the qPCR was confirmed by testing the DNA extracts from a panel of microorganisms that were either common fish pathogens or reported to cause false-positive reactions in the enzyme-linked immunosorbent assay (ELISA). Kidney samples from 38 juvenile Chinook salmon (Oncorhynchus tshawytscha) in a naturally infected population were examined by real-time qPCR, a nested PCR, and ELISA, and prevalences of R. salmoninarum detected were 71, 66, and 71%, respectively. The qPCR should be a valuable tool for evaluating the R. salmoninarum infection status of salmonids.

  8. Potential effects of climate change on streambed scour and risks to salmonid survival in snow-dominated mountain basins

    Treesearch

    Jaime R. Goode; John M. Buffington; Daniele Tonina; Daniel J. Isaak; Russell F. Thurow; Seth Wenger; David Nagel; Charlie Luce; Doerthe Tetzlaff; Chris Soulsby

    2013-01-01

    Snowmelt-dominated basins in northern latitudes provide critical habitat for salmonids. As such, these systems may be especially vulnerable to climate change because of potential shifts in the frequency, magnitude, and timing of flows that can scour incubating embryos. A general framework is presented to examine this issue, using a series of physical models that link...

  9. Predation on Pacific salmonid eggs and carcass's by subyearling Atlantic salmon in a tributary of Lake Ontario

    USGS Publications Warehouse

    Johnson, James H.; Chalupnicki, Marc A.; Abbett, Ross; Verdoliva, Francis

    2016-01-01

    A binational effort to reintroduce Atlantic salmon (Salmo salar) that were extirpated in the Lake Ontario ecosystem for over a century is currently being undertaken by the New York State Department of Environmental Conservation and the Ontario Ministry of Natural Resources. Reintroduction actions include the release of several life stages including fry, fall fingerlings, and yearling smolts. In this study we describe the diet of recently released fall fingerling Atlantic salmon in a tributary of the Salmon River, New York. A specific objective of the study was to determine if juvenile Atlantic salmon would utilize the high caloric food source provided by introduced Pacific salmonids (Oncorhynchus spp.) that includes eggs and carcass flesh. Salmon eggs and carcass flesh comprised 20.5% of the October to January diet in 2013–14 and 23.9% in 2014–15. The consumption of steelhead (O. mykiss) eggs was a major part of the diet in April in both 2014 (54.1%) and 2015 (33.2%). This study documented that recently released Atlantic salmon will consume the high caloric food material provided by Pacific salmonids and that the consumption of this material extends for several months.

  10. Functional morphology and biomechanics of the tongue-bite apparatus in salmonid and osteoglossomorph fishes

    PubMed Central

    Camp, Ariel L; Konow, Nicolai; Sanford, Christopher P J

    2009-01-01

    The tongue-bite apparatus and its associated musculoskeletal elements of the pectoral girdle and neurocranium form the structural basis of raking, a unique prey-processing behaviour in salmonid and osteoglossomorph fishes. Using a quantitative approach, the functional osteology and myology of this system were compared between representatives of each lineage, i.e. the salmonid Salvelinus fontinalis (N =10) and the osteoglossomorph Chitala ornata(N = 8). Divergence was found in the morphology of the novel cleithrobranchial ligament, which potentially relates to kinematic differences between the raking lineage representatives. Salvelinus had greater anatomical cross-sectional areas of the epaxial, hypaxial and protractor hyoideus muscles, whereas Chitala had greater sternohyoideus and adductor mandibulae mass. Two osteology-based biomechanical models (a third-order lever for neurocranial elevation and a modified four-bar linkage for hyoid retraction) showed divergent force/velocity priorities in the study taxa. Salvelinus maximizes both force (via powerful cranial muscles) and velocity (through mechanical amplification) during raking. In contrast, Chitala has relatively low muscle force but more efficient force transmission through both mechanisms compared with Salvelinus. It remains unclear if and how behavioural modulation and specializations in the post-cranial anatomy may affect the force/velocity trade-offs in Chitala. Further studies of tongue-bite apparatus morphology and biomechanics in a broader species range may help to clarify the role that osteology and myology play in the evolution of behavioural diversity. PMID:19438765

  11. A review of factors influencing the availability of dissolved oxygen to incubating salmonid embryos

    NASA Astrophysics Data System (ADS)

    Greig, S. M.; Sear, D. A.; Carling, P. A.

    2007-01-01

    Previous investigations into factors influencing incubation success of salmonid progeny have largely been limited to the development of empirical relationships between characteristics of the incubation environment and survival to emergence. It is suggested that adopting a process-based approach to assessing incubation success aids identification of the precise causes of embryonic mortalities, and provides a robust framework for developing and implementing managerial responses.Identifying oxygen availability within the incubation environment as a limiting factor, a comprehensive review of trends in embryonic respiration, and processes influencing the flux of oxygenated water through gravel riverbeds is provided. The availability of oxygen to incubating salmonid embryos is dependent on the exchange of oxygenated water with the riverbed, and the ability of the riverbed gravel medium to transport this water at a rate and concentration appropriate to support embryonic respiratory requirements. Embryonic respiratory trends indicate that oxygen consumption varies with stage of development, ambient water temperature and oxygen availability. The flux of oxygenated water through the incubation environment is controlled by a complex interaction of intragravel and extragravel processes and factors. The processes driving the exchange of channel water with gravel riverbeds include bed topography, bed permeability, and surface roughness effects. The flux of oxygenated water through riverbed gravels is controlled by gravel permeability, coupling of surface-subsurface flow and oxygen demands imposed by materials infiltrating riverbed gravels. Temporally and spatially variable inputs of groundwater can also influence the oxygen concentration of interstitial water. Copyright

  12. Islands in the ice stream: were spawning habitats for native salmonids in the Great Lakes created by paleo-ice streams?

    USGS Publications Warehouse

    Riley, Stephen; Binder, Thomas R.; Tucker, Taaja R.; Menzies, John; Eyles, Nick; Janssen, John; Muir, Andrew M.; Esselman, Peter C.; Wattrus, Nigel J.; Krueger, Charles C.

    2016-01-01

    Lake trout Salvelinus namaycush, lake whitefish Coregonus clupeaformis and cisco Coregonus artedi are salmonid fishes native to the Laurentian Great Lakes that spawn on rocky substrates in the fall and early winter. After comparing the locations of spawning habitat for these species in the main basin of Lake Huron with surficial substrates and the hypothesized locations of fast-flowing Late Wisconsinan paleo-ice streams, we hypothesize that much of the spawning habitat for these species in Lake Huron is the result of deposition and erosion by paleo-ice streams. This hypothesis may represent a new framework for the identification and protection of spawning habitat for these native species, some of which are currently rare or extirpated in some of the Great Lakes. We further suggest that paleo-ice streams may have been responsible for the creation of native salmonid spawning habitat elsewhere in the Great Lakes and in other glaciated landscapes.

  13. Inhibitory activity of (E)-5-(2-bromovinyl)-2'-deoxyuridine on the salmonid herpesviruses, Oncorhynchus masou virus (OMV) and Herpesvirus salmonis.

    PubMed

    Kimura, T; Nishizawa, T; Yoshimizu, M; De Clercq, E

    1988-01-01

    The highly potent and selective anti-herpesvirus agent, (E)-5-(2-bromovinyl)-2'deoxyuridine (BVdU), was examined for its inhibitory effect on the salmonid herpesviruses Oncorhynchus masou virus (OMV) and Herpesvirus salmonis (H. salmonis). Minimum inhibitory concentrations (MIC) of BVdU for OMV and H. salmonis were 1.25 and 3.0 micrograms/ml, respectively; these values were equal to or higher than those obtained for acyclovir or cytarabine. OMV DNA polymerase activity was reduced in a dose-dependent fashion by BVdU 5'-triphosphate (BVdUTP) within the concentration range of 3 to 30 microM. However, BVdUTP could also be substituted for the natural substrate, TTP, in the OMV DNA polymerase assay. It is postulated that the inhibitory action of BVdU on the salmonid herpesviruses is more or less similar to that on other herpesviruses and resides with respect to the inhibition of the virus DNA polymerase activity as well as incorporation of BVdU into the viral DNA.

  14. System-Wide Significance of Predation on Juvenile Salmonids in the Columbia and Snake River Reservoirs : Annual Report of Research 1991.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shively, R.S.

    1991-01-01

    We indexed consumption rates of northern squawfish (Ptychoch oregonensis) preying upon juvenile salmonids in four lower Snake River reservoirs. Stomach contents were also collected from smallmouth bass (Micropterus dolomieui), channel catfish (Ictaluris gunctatus), and walleye (Stizostedion vitreum). Northern squawfish digestive tracts were analyzed and the overall diet (% weight) was dominated by fish and crustaceans. Examination of stomach contents smallmouth bass showed that crustaceans (primarily crayfish) dominated their diets. Overall, the consumption rate of juvenile salmonids by smallmouth bass was low. The northern squawfish consumption index (CI) at Snake River locations ranged from zero at all mid-reservoir locations to 1.2more » at Lower Granite forebay. In John Day Reservoir, CI values ranged from 0.5 to 1.9 in May and from 0.9 to 3.0 in July. Consumption index values were highest in forebay and tailrace areas, and were slightly higher in BRZs than in non-restricted zones.« less

  15. Determination of supplemental feeding needs for astaxanthin and canthaxanthin in salmonids by supramolecular solvent-based microextraction and liquid chromatography-UV/VIS spectroscopy.

    PubMed

    Caballo, Carmen; Costi, Esther María; Sicilia, María Dolores; Rubio, Soledad

    2012-09-15

    Development of simple and rapid analytical methods for predicting supplemental feeding requirements in aquaculture is a need to reduce production costs. In this article, a supramolecular solvent (SUPRAS) made up of decanoic acid (DeA) assemblies was proposed to simplify sample treatment in the total and individual determination of carotenoids (red-pink pigments) in farmed salmonids. The analytes were quantitatively extracted in a single step that spends a few minutes using a small volume of SUPRAS (i.e. 800 μL) and directly determined in extracts without the interference from fats or other matrix components. The methods based on the combination of microextraction with SUPRAS and photometry or HPLC-UV/VIS spectroscopy were developed for the determination of total and individual carotenoids, respectively. The applicability of the methods was demonstrated by analysing non-fortified and fortified samples of farmed Atlantic salmons and rainbow trouts. Recoveries obtained by photometry and HPLC-UV/VIS spectroscopy were within the intervals 98-104% and 94-106%, respectively. Copyright © 2012 Elsevier Ltd. All rights reserved.

  16. Development and corroboration of a bioenergetics model for northern pikeminnow (Ptychocheilus oregonensis) feeding on juvenile salmonids in the Columbia River

    USGS Publications Warehouse

    Petersen, J.H.; Ward, D.L.

    1999-01-01

    A bioenergetics model was developed and corroborated for northern pikeminnow Ptychocheilus oregonensis, an important predator on juvenile salmonids in the Pacific Northwest. Predictions of modeled predation rate on salmonids were compared with field data from three areas of John Day Reservoir (Columbia River). To make bioenergetics model estimates of predation rate, three methods were used to approximate the change in mass of average predators during 30-d growth periods: observed change in mass between the first and the second month, predicted change in mass calculated with seasonal growth rates, and predicted change in mass based on an annual growth model. For all reservoir areas combined, bioenergetics model predictions of predation on salmon were 19% lower than field estimates based on observed masses, 45% lower than estimates based on seasonal growth rates, and 15% lower than estimates based on the annual growth model. For each growth approach, the largest differences in field-versus-model predation occurred at the midreservoir area (-84% to -67% difference). Model predictions of the rate of predation on salmonids were examined for sensitivity to parameter variation, swimming speed, sampling bias caused by gear selectivity, and asymmetric size distributions of predators. The specific daily growth rate of northern pikeminnow predicted by the model was highest in July and October and decreased during August. The bioenergetics model for northern pikeminnow performed well compared with models for other fish species that have been tested with field data. This model should be a useful tool for evaluating management actions such as predator removal, examining the influence of temperature on predation rates, and exploring interactions between predators in the Columbia River basin.

  17. Complete genome sequence of Yersinia ruckeri str. CSF007-82, etiologic agent of enteric redmouth disease in salmonid fish

    USDA-ARS?s Scientific Manuscript database

    We present the complete, closed and finished chromosomal and extra-chromosomal genome sequences of Y. ruckeri strain CSF007-82, etiologic agent of enteric red mouth disease in salmonid fish. The chromosome is 3,799,036 bp with a G+C content of 47.5% and encodes 3,530 predicted CDS, 7 ribosomal opero...

  18. Infection experiments with novel Piscine orthoreovirus from rainbow trout (Oncorhynchus mykiss) in salmonids

    PubMed Central

    Taksdal, Torunn; Olsen, Anne Berit; Wessel, Øystein; Mikkelsen, Susie Sommer; Alencar, Anna Luiza Farias; Olesen, Niels Jørgen

    2017-01-01

    A new disease in farmed rainbow trout (Onchorhyncus mykiss) was described in Norway in 2013. The disease mainly affected the heart and resembled heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar L.). HSMI is associated with Piscine orthoreovirus (PRV), and a search for a similar virus in the diseased rainbow trout led to detection of a sequence with 85% similarity to PRV. This finding called for a targeted effort to assess the risk the new PRV-variant pose on farmed rainbow trout and Atlantic salmon by studying infection and disease pathogenesis, aiming to provide more diagnostic knowledge. Based on the genetic relationship to PRV, the novel virus is referred to as PRV-Oncorhynchus mykiss (PRV-Om) in contrast to PRV-Salmo salar (PRV-Ss). In experimental trials, intraperitoneally injected PRV-Om was shown to replicate in blood in both salmonid species, but more effectively in rainbow trout. In rainbow trout, the virus levels peaked in blood and heart of cohabitants 6 weeks post challenge, along with increased expression of antiviral genes (Mx and viperin) in the spleen, with 80–100% of the cohabitants infected. Heart inflammation was diagnosed in all cohabitants examined 8 weeks post challenge. In contrast, less than 50% of the Atlantic salmon cohabitants were infected between 8 and 16 weeks post challenge and the antiviral response in these fish was very low. From 12 weeks post challenge and onwards, mild focal myocarditis was demonstrated in a few virus-positive salmon. In conclusion, PRV-Om infects both salmonid species, but faster transmission, more notable antiviral response and more prominent heart pathology were observed in rainbow trout. PMID:28678799

  19. Effectiveness of a redesigned water diversion using rock vortex weirs to enhance longitudinal connectivity for small Salmonids

    USGS Publications Warehouse

    Martens, Kyle D.; Connolly, Patrick J.

    2010-01-01

    For nearly 100 years, water diversions have affected fish passage in Beaver Creek, a tributary of the lower Methow River in north-central Washington State. From 2000 to 2004, four dam-style water diversions were replaced with a series of rock vortex weirs (RVWs). The weirs were designed to allow fish passage while maintaining the ability to divert water into irrigation canals. We observed the new appearance of three species (juvenile Chinook salmon Oncorhynchus tshawytscha, juvenile coho salmon O. kisutch, and mountain whitefish Prosopium williamsoni) upstream of the RVWs, indicating successful restoration of longitudinal connectivity. We used passive integrated transponder (PIT) tags and instream PIT tag interrogation systems during 2004–2007 to evaluate upstream passage of small salmonids (<240 mm fork length) through one series of RVWs. We documented 109 upstream passage events by small salmonids through the series of RVWs; most of the events (81%) involved passage of rainbow trout O. mykiss or juvenile steelhead (anadromous rainbow trout). Small rainbow trout or steelhead ranging from 86 to 238 mm (adjusted fork length) were able to pass upstream through the RVWs, although a delay in fish passage at discharges below 0.32 m3/s was detected in comparison with nearby control sections.

  20. Hydroacoustic Evaluation of Juvenile Salmonid Passage at The Dalles Dam Sluiceway, 2005

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Johnson, Gary E.; Khan, Fenton; Hedgepeth, J

    2006-06-01

    The U.S. Army Corps of Engineers Portland District engaged the Pacific Northwest National Laboratory to evaluate fish passage at The Dalles Dam powerhouse in 2005. The goal of the study was to provide information on smolt passage that will inform decisions on long-term measures and operations to enhance sluiceway passage and reduce turbine passage to improve smolt survival at the dam. The study addressed one of the main programs dedicated to improving juvenile salmonid survival at The Dalles Dam: Surface Flow Bypass. The study objectives (see below) were met using a combination of hydroacoustic and hydraulic data. The study incorporatedmore » fixed-location hydroacoustic methods across the entire powerhouse, with especially intense sampling using multiple split-beam transducers at all sluiceway portals. We did not sample fish passage at the spillway in 2005. In the sluiceway nearfield, we used an acoustic camera to track fish movements. The fish data were interpreted with hydraulic data from a computational fluid dynamics (CFD) model. Fish passage data were collected in the framework of an “experiment” using a randomized block design (3-day treatments; two treatments) to compare two sluiceway operational configurations: Sluice 2+5 and Sluice 2+19 (six gates open for each configuration). Total project outflow was 76% of the 10-year average for spring and 71% of the 10-year average for summer. Based on these findings, we make the following recommendations: 1) The sluice should be operated 24 h/d from April until November. 2) Open six rather than three sluice gates to take advantage of the maximum hydraulic capacity of the sluiceway. 3) Open the three gates above the western-most operating main turbine unit and the three gates at MU 8 where turbine passage rates are relatively high. 4) Operate the turbine units below open sluice gates as a standard fish operations procedure. 5) Develop hydraulic and entrance enhancements to the sluiceway to tap the potential of

  1. Comparative evaluation of molecular diagnostic tests for Nucleospora salmonis and prevalence in migrating juvenile salmonids from the Snake River, USA

    USGS Publications Warehouse

    Badil, Samantha; Elliott, Diane G.; Kurobe, Tomofumi; Hedrick, Ronald P.; Clemens, Kathy; Blair, Marilyn; Purcell, Maureen K.

    2011-01-01

    Nucleospora salmonis is an intranuclear microsporidian that primarily infects lymphoblast cells and contributes to chronic lymphoblastosis and a leukemia-like condition in a range of salmonid species. The primary goal of this study was to evaluate the prevalence of N. salmonis in out-migrating juvenile hatchery and wild Chinook salmon Oncorhynchus tshawytscha and steelhead O. mykiss from the Snake River in the U.S. Pacific Northwest. To achieve this goal, we first addressed the following concerns about current molecular diagnostic tests for N. salmonis: (1) nonspecific amplification patterns by the published nested polymerase chain reaction (nPCR) test, (2) incomplete validation of the published quantitative PCR (qPCR) test, and (3) whether N. salmonis can be detected reliably from nonlethal samples. Here, we present an optimized nPCR protocol that eliminates nonspecific amplification. During validation of the published qPCR test, our laboratory developed a second qPCR test that targeted a different gene sequence and used different probe chemistry for comparison purposes. We simultaneously evaluated the two different qPCR tests for N. salmonis and found that both assays were highly specific, sensitive, and repeatable. The nPCR and qPCR tests had good overall concordance when DNA samples derived from both apparently healthy and clinically diseased hatchery rainbow trout were tested. Finally, we demonstrated that gill snips were a suitable tissue for nonlethal detection of N. salmonis DNA in juvenile salmonids. Monitoring of juvenile salmonid fish in the Snake River over a 3-year period revealed low prevalence of N. salmonis in hatchery and wild Chinook salmon and wild steelhead but significantly higher prevalence in hatchery-derived steelhead. Routine monitoring of N. salmonis is not performed for all hatchery steelhead populations. At present, the possible contribution of this pathogen to delayed mortality of steelhead has not been determined.

  2. Ichthyophonus-induced cardiac damage: a mechanism for reduced swimming stamina in salmonids.

    PubMed

    Kocan, R; Lapatra, S; Gregg, J; Winton, J; Hershberger, P

    2006-09-01

    Swimming stamina, measured as time-to-fatigue, was reduced by approximately two-thirds in rainbow trout experimentally infected with Ichthyophonus. Intensity of Ichthyophonus infection was most severe in cardiac muscle but multiple organs were infected to a lesser extent. The mean heart weight of infected fish was 40% greater than that of uninfected fish, the result of parasite biomass, infiltration of immune cells and fibrotic (granuloma) tissue surrounding the parasite. Diminished swimming stamina is hypothesized to be due to cardiac failure resulting from the combination of parasite-damaged heart muscle and low myocardial oxygen supply during sustained aerobic exercise. Loss of stamina in Ichthyophonus-infected salmonids could explain the poor performance previously reported for wild Chinook and sockeye salmon stocks during their spawning migration.

  3. Understanding the influence of predation by introduced fishes on juvenile salmonids in the Columbia River Basin: Closing some knowledge gaps. Interim Report of Research 2010

    USGS Publications Warehouse

    Rose, Brien P.; Hansen, Gabriel S.; Mesa, Matthew G.

    2011-01-01

    In response to these recent concerns about the potential predatory impact of non-native piscivores on salmon survival, the Bonneville Power Administration (BPA) and the Columbia Basin Fish and Wildlife Authority (CBFWA) co-hosted a workshop to address predation on juvenile salmonids in the CRB by non-native fish (Halton 2008). The purpose of the workshop was to review, evaluate, and develop strategies to reduce predation by non-native fishes on juvenile salmonids. In the end, discussion at the workshop and at subsequent meetings considered two potential ideas to reduce predation by non-native fish on juvenile salmonids; (1) understanding the role of juvenile American shad Alosa sapidissima in the diet of non-native predators in the fall; and (2) the effects of localized, intense reductions of smallmouth bass in areas of particularly high salmonid predation. In this report, we describe initial efforts to understand the influence of juvenile American shad as a prey item for introduced predators in the middle Columbia River. Our first objective, addressed in Chapter 1, was to evaluate the efficacy of nonlethal methods to describe the physiological condition of smallmouth bass, walleye, and channel catfish from late summer through late fall. Such information will be used to understand the contribution of juvenile American shad to the energy reserves of predaceous fish prior to winter. In Chapter 2, we describe the results of some limited sampling to document the food habits of smallmouth bass, walleye, and channel catfish in three reservoirs of the middle Columbia River during late fall. Collectively, we hope to increase our understanding of the contribution of juvenile American shad to the diets of introduced predators and the contribution of this diet to their energy reserves, growth, and perhaps over-winter survival. Managers should be able to use this information for deciding whether to control the population of American shad in the CRB or for managing introduced

  4. Genetic characterization of infectious hematopoietic necrosis virus of coastal salmonid stocks in Washington State

    USGS Publications Warehouse

    Emmenegger, E.J.; Kurath, G.

    2002-01-01

    Infectious hematopoietic necrosis virus (IHNV) is a pathogen that infects many Pacific salmonid stocks from the watersheds of North America. Previous studies have thoroughly characterized the genetic diversity of IHNV isolates from Alaska and the Hagerman Valley in Idaho. To enhance understanding of the evolution and viral transmission patterns of IHNV within the Pacific Northwest geographic range, we analyzed the G gene of IHNV isolates from the coastal watersheds of Washington State by ribonuclease protection assay (RPA) and nucleotide sequencing. The RPA analysis of 23 isolates indicated that the Skagit basin IHNV isolates were relatively homogeneous as a result of the dominance of one G gene haplotype (S). Sequence analysis of 303 bases in the middle of the G gene (midG region) of 61 isolates confirmed the high frequency of a Skagit River basin sequence and identified another sequence commonly found in isolates from the Lake Washington basin. Overall, both the RPA and sequence analysis showed that the Washington coastal IHNV isolates are genetically homogeneous and have little genetic diversity. This is similar to the genetic diversity pattern of IHNV from Alaska and contrasts sharply with the high genetic diversity demonstrated for IHNV isolates from fish farms along the Snake River in Idaho. The high degree of sequence and haplotype similarity between the Washington coastal IHNV isolates and those from Alaska and British Columbia suggests that they have a common viral ancestor. Phylogenetic analyses of the isolates we studied and those from different regions throughout the virus's geographic range confirms a conserved pattern of evolution of the virus in salmonid stocks north of the Columbia River, which forms Washington's southern border.

  5. Distribution and status of seven native salmonids in the interior Columbia River basin and portions of the Klamath River and Great basins

    Treesearch

    Russell F. Thurow; Danny C. Lee; Bruce E. Rieman

    1997-01-01

    We summarized presence, absence, current status, and potential historical distribution of seven native salmonid taxa - bull trout Salvelinus confluentus, Yellowstone cutthroat trout Oncorhynchus clarki bouvieri, westslope cutthroat trout O. c. lewisi, redband trout and steelhead O. mykiss gairdneri, stream type (age-1 migrant) chinook salmon O. tshawytscha. and ocean...

  6. Biochemical and antigenic properties of the first isolates of infectious hematopoietic necrosis virus from salmonid fish in Europe

    USGS Publications Warehouse

    Arkush, K.D.; Bovo, G.; DeKinkelin, P.; Winton, J.R.; Wingfield, W.H.; Hedrick, R.P.

    1989-01-01

    The first isolates of infectious hematopoietic necrosis virus (IHNV) recovered from rainbow trout Oncorhynchus mykiss (formerly Salmo gairdneri) in France and Italy were compared to six representative strains from North America by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of virion polypeptides and neutralization by monoclonal antibodies (MAbs). All three IHNV isolates from Europe had similar polypeptide profiles when compared by SDS-PAGE. An analysis of the antigenic relatedness of the European isolates to representative strains from North America showed that they were clearly different from viruses obtained from salmonids in California. The RB/B5 MAb, which was developed against virus isolated from adult steelhead (anadromous rainbow trout) reared in central Oregon, neutralized all isolates examined. The 193–110/B4 MAb, developed against IHNV isolated from infected yearling rainbow trout in southern Idaho, neutralized all isolates tested except those from California. The SRCV/A4 MAb, developed against Sacramento River chinook virus (SRCV) isolated from adult spring chinook salmon O. tshawytscha in central California, was the least reactive, and strong neutralization was observed only with the SRCV strain of IHNV from California. However, partial reactivity of the virus isolates from France with the SRCV/A4 MAb distinguished them from the virus recovered from salmonids in Italy.

  7. The Long-Term Effects of Large Wood Placement on Salmonid Habitat in East Fork Mill Creek, Redwood National and State Park, California

    NASA Astrophysics Data System (ADS)

    Rodriguez, D. L.; Stubblefield, A. P.

    2017-12-01

    The conservation and recovery of anadromous salmonids (Oncorhynchus sp.) depend on stream restoration and protection of freshwater habitats. Instream large wood dictates channel morphology, increase retention of terrestrial inputs such as organic matter, nutrients and sediment, and enhances the quality of fish habitat. Historic land use/land cover changes have resulted in aquatic systems devoid of this component. Restoration by placement of large wood jams is intended to restore physical and biological processes. An important question for scientists and managers, in addition to the initial effectiveness of restoration, is the persistence and fate of this type of project. In this study we compare channel change and large wood attributes on the East Fork of Mill Creek, a tributary of the Smith River in northern California, eight years after a major instream wood placement effort took place. Our results are compared with previously published data from before and one year after the restoration. Preliminary results suggest the dramatic increase in spawning gravel abundance and large wood accumulation observed in the earlier study have persisted. From 2008 to 2016 a reduction in median sediment size, ranging from 103-136 percent, has been observed in a majority of the sites. The sites have continued to grow in size and influence by racking floating wood from upstream and destabilizing proximate banks of riparian alder, increasing both instream large wood volume (5-196 %) and floodplain connectivity. Preliminary results also show a decrease in residual pool depth and an increase in pool length which may be attributed to floodplain connectivity. Changes to the following attributes are evaluated: 1) wood loading (total site wood volume, total wood volume in active channel, and wood piece count); 2) percent pool cover by large wood; 3) residual pool depth; 4) upstream sediment aggradation; 5) floodplain connectivity; and 6) mean sediment size directly above and below large

  8. Genome specific PPARαB duplicates in salmonids and insights into estrogenic regulation in brown trout.

    PubMed

    Madureira, Tânia Vieira; Pinheiro, Ivone; de Paula Freire, Rafaelle; Rocha, Eduardo; Castro, Luis Filipe; Urbatzka, Ralph

    2017-06-01

    Peroxisome proliferator-activated receptors (PPARs) are key regulators of many processes in vertebrates, such as carbohydrate and lipid metabolism. PPARα, a member of the PPAR nuclear receptor gene subfamily (NR1C1), is involved in fatty acid metabolism, namely in peroxisomal β-oxidation. Two gene paralogues, pparαA and pparαB, were described in several teleost species with their origin dating back to the teleost-specific genome duplication (3R). Given the additional salmonid-specific genome duplication (4R), four genes could be theoretically anticipated for this gene subfamily. In this work, we examined the pparα gene repertoire in brown trout, Salmo trutta f. fario. Data disclosed two pparα-like sequences in brown trout. Phylogenetic analyses further revealed that the isolated genes are most likely genome pparαB duplicates, pparαBa and pparαBb, while pparαA is apparently absent in salmonids. Both genes showed a ubiquitous mRNA expression across a panel of 11 different organs. In vitro exposed primary brown trout hepatocytes strongly suggest that pparα gene paralogues are differently regulated by ethinylestradiol (EE2). PparαBb mRNA expression significantly decreased with dosage, reaching significance after exposure to 50μM EE2, while pparαBa mRNA increased, significant at 1μM EE2. The present data enhances the understanding of pparα function and evolution in teleost, and reinforces the evidence of a potential crosstalk between estrogenic and pparα signaling pathways. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. Investigating the Geomorphic and Ecologic Functions of Wood in Relationship to Habitat Type and Salmonid Redds on a Regulated California River

    NASA Astrophysics Data System (ADS)

    Senter, A. E.; Pasternack, G. B.

    2006-12-01

    Most river rehabilitation projects incorporate little to no wood in current designs, and those that do have little science to guide them. The overall goal of this research is to investigate the role of wood in a regulated, mid- sized (where river width is greater than most tree heights), Mediterranean-climate (where smaller, softer-wood trees dominate the landscape) river in order to provide a scientific foundation for the potential use of wood in rehabilitation projects within such systems. Wood structures in the active salmonid spawning reach of the Lower Mokelumne River in Central California were measured, mapped, and described during summer and fall 2006. Digital photos and GPS coordinates were used to establish wood location within the stream channel. Structural morphology was determined by measuring physical properties such as individual diameter and length, orientation to stream flow, and jam dimensions. In addition, qualitative attributes were recorded such as decay class and leaf, limb, bark, and root characteristics. A GIS wood layer will be created and added to a database of existing Mokelumne River GIS layers containing salmonid redd (salmon egg nests) densities, hydraulic conditions associated with individual redds, and sub-reach habitat types. An analysis of wood properties, redd locations and conditions, and habitat types will be used to develop a conceptual model of wood dynamics in relation to salmonid habitat on the Lower Mokelumne River. The primary products of this study will be (1) a scientific conceptual model of the role of wood in regulated gravel reaches of mid-size rivers in Mediterranean California and (2) a decision-making framework that will enable river managers to include scientifically based wood structures into rehabilitation designs, thereby enhancing spawning habitat, stream complexity, and biological diversity. These tools will be developed in collaboration with East Bay Municipal Utilities District to aid in the continuing

  10. Using Satellite Tracking and Isotopic Information to Characterize the Impact of South American Sea Lions on Salmonid Aquaculture in Southern Chile.

    PubMed

    Sepúlveda, Maritza; Newsome, Seth D; Pavez, Guido; Oliva, Doris; Costa, Daniel P; Hückstädt, Luis A

    2015-01-01

    Apex marine predators alter their foraging behavior in response to spatial and/or seasonal changes in natural prey distribution and abundance. However, few studies have identified the impacts of aquaculture that represents a spatially and temporally predictable and abundant resource on their foraging behavior. Using satellite telemetry and stable isotope analysis we examined the degree of spatial overlap between the South American sea lion (SASL) and salmon farms, and quantify the amount of native prey versus farmed salmonids in SASL diets. We instrumented eight SASL individuals with SRDL-GPS tags. Vibrissae, hair and skin samples were collected for δ13C and δ15N analyses from five of the tagged individuals and from four males captured in a haul-out located adjacent to salmon farms. Tracking results showed that almost all the foraging areas of SASL are within close proximity to salmon farms. The most important prey for the individuals analyzed was farmed salmonids, with an estimated median (±SD) contribution of 19.7 ± 13.5‰ and 15.3 ± 9.6‰ for hair and skin, respectively. Using vibrissae as a temporal record of diet for each individual, we observed a remarkable switch in diet composition in two SASL, from farmed salmonids to pelagic fishes, which coincided with the decrease of salmon production due to the infectious salmon anemia virus that affected salmon farms in Chile at the end of 2008. Our study demonstrates the usefulness of integrating stable isotope derived dietary data with movement patterns to characterize the impacts of a non-native prey on the foraging ecology of an apex marine predator, providing important applied implications in situations where interactions between aquaculture and wildlife are common.

  11. Using Satellite Tracking and Isotopic Information to Characterize the Impact of South American Sea Lions on Salmonid Aquaculture in Southern Chile

    PubMed Central

    Sepúlveda, Maritza; Newsome, Seth D.; Pavez, Guido; Oliva, Doris; Costa, Daniel P.; Hückstädt, Luis A.

    2015-01-01

    Apex marine predators alter their foraging behavior in response to spatial and/or seasonal changes in natural prey distribution and abundance. However, few studies have identified the impacts of aquaculture that represents a spatially and temporally predictable and abundant resource on their foraging behavior. Using satellite telemetry and stable isotope analysis we examined the degree of spatial overlap between the South American sea lion (SASL) and salmon farms, and quantify the amount of native prey versus farmed salmonids in SASL diets. We instrumented eight SASL individuals with SRDL-GPS tags. Vibrissae, hair and skin samples were collected for δ13C and δ15N analyses from five of the tagged individuals and from four males captured in a haul-out located adjacent to salmon farms. Tracking results showed that almost all the foraging areas of SASL are within close proximity to salmon farms. The most important prey for the individuals analyzed was farmed salmonids, with an estimated median (±SD) contribution of 19.7 ± 13.5‰ and 15.3 ± 9.6‰ for hair and skin, respectively. Using vibrissae as a temporal record of diet for each individual, we observed a remarkable switch in diet composition in two SASL, from farmed salmonids to pelagic fishes, which coincided with the decrease of salmon production due to the infectious salmon anemia virus that affected salmon farms in Chile at the end of 2008. Our study demonstrates the usefulness of integrating stable isotope derived dietary data with movement patterns to characterize the impacts of a non-native prey on the foraging ecology of an apex marine predator, providing important applied implications in situations where interactions between aquaculture and wildlife are common. PMID:26309046

  12. Are brown trout Salmo trutta fario and rainbow trout Oncorhynchus mykiss two of a kind? A comparative study of salmonids to temperature-influenced Tetracapsuloides bryosalmonae infection.

    PubMed

    Bailey, C; Schmidt-Posthaus, H; Segner, H; Wahli, T; Strepparava, N

    2018-02-01

    Proliferative kidney disease (PKD) of salmonids caused by Tetracapsuloides bryosalmonae causes high mortalities of wild brown trout (Salmo trutta fario) and farmed rainbow trout (Oncorhynchus mykiss) at elevated water temperatures. Here the aim was to compare the temperature-dependent modulation of T. bryosalmonae in the two salmonid host species, which display different temperature optima. We used a novel experimental set-up in which we exposed brown trout and rainbow trout to an identical quantified low concentration of T. bryosalmonae for a short time period (1 hr). We followed the development of the parasite in the fish hosts for 70 days. PKD prevalence and parasite kinetics were assessed using qPCR. Exposures were performed at temperatures (12°C and 15°C) that reflect an environmental scenario that may occur in the natural habitat of salmonids. T. bryosalmonae infection was confirmed earliest in brown trout kept at 15°C (day 7 post-exposure) while, in all other groups, T. bryosalmonae was not confirmed until day 15 post-exposure. Moreover, significantly greater infection prevalence and a faster increase of parasite intensity were observed in brown trout kept at 15°C than in all other groups. These results indicate that PKD is differentially modulated by water temperature in related host species. © 2017 John Wiley & Sons Ltd.

  13. Efficacy, fate, and potential effects on salmonids of mosquito larvicides in catch basins in Seattle, Washington

    USGS Publications Warehouse

    Sternberg, Morgan; Grue, Christian; Conquest, Loveday; Grassley, James; King, Kerensa

    2012-01-01

    We investigated the efficacy, fate, and potential for direct effects on salmonids of 4 common mosquito larvicides (Mosquito Dunks® and Bits® (AI: Bacillis thuringiensis var. israelensis, [Bti]), VectoLex® WSP (AI: Bacillus sphaericus [Bs], VectoLex CG [AI: Bs], and Altosid® Briquets [AI: s-methoprene]) in Seattle, WA, during 3 summers. During efficacy trials in 2006, all treatments resulted in a rapid reduction in number of mosquito pupae (Mosquito Dunks and Bits and VectoLex WSP) or emergence success (Altosid Briquets). VectoLex CG was chosen for city-wide application in 2007 and 2008. The average counts of pupae within round-top basins remained significantly below the control average for 11 wk in 2007, whereas efficacy in grated-top basins was short-lived. In 2008 the average counts of pupae within grated-top basins remained significantly below the control average for 10 wk. Altosid XR was also effective in reducing adult emergence within grated basins in 2008. In 2007 and 2008, frequent precipitation events made the evaluation of efficacy difficult due to reductions in pupae across control and treated basins. Four separate analyses of VectoLex products revealed that the product was a combination of Bs and Bti. Both Bs and Bti were detected in 3 urban creeks connected to treated basins in 2007 and 2008. Laboratory toxicity test results suggest that concentrations of Bs and Bti detected in each of the watersheds pose little direct hazard to juvenile salmonids.

  14. Efficacy of passive sand filtration in reducing exposure of salmonids to the actinospore of Myxobolus cerebralis.

    PubMed

    Nehring, R Barry; Thompson, Kevin G; Taurman, Karen; Atkinson, William

    2003-12-03

    The aquatic oligochaete Tubifex tubifex parasitized by Myxobolus cerebralis releases triactinomyxon (TAM) actinospores that can infect some species of salmonids and cause salmonid whirling disease. Silica sand was tested as a filtration medium for removal of TAMs from water containing the parasite. Laboratory tests indicated sand filtration removed > 99.99% of TAMs. In 2 different field tests, groups of 1 mo old rainbow trout Oncorhynchus mykiss were exposed for 2 wk to filtered and unfiltered water from a spring-fed pond enzootic for M. cerebralis. In November 2000, the exposure dose was estimated as between 3 and 5 TAMs fish(-1). During a March 2001 exposure, the estimated dose was between 286 and 404 TAMs fish(-1). Fish were held for 6 mo post exposure (p.e.) in laboratory aquaria for observation and evidence of clinical signs of whirling disease. We used 4 diagnostic techniques to assess the prevalence and severity of infection by M. cerebralis among fish exposed to filtered and unfiltered water. These included polymerase chain reaction (PCR) for genomic DNA of the parasite, histological evaluation for tissue damage, tissue digestion for quantification of cranial myxospores of the parasite, and total non-sampling mortality that occurred over 6 mo p.e. All diagnostic tests verified that the prevalence and severity of infection was significantly reduced among fish in treatment groups exposed to filtered water compared to those exposed to unfiltered water in both the low-dose and high-dose exposures.

  15. The waterfall paradox: How knickpoints disconnect hillslope and channel processes, isolating salmonid populations in ideal habitats

    NASA Astrophysics Data System (ADS)

    May, Christine; Roering, Josh; Snow, Kyle; Griswold, Kitty; Gresswell, Robert

    2017-01-01

    Waterfalls create barriers to fish migration, yet hundreds of isolated salmonid populations exist above barriers and have persisted for thousands of years in steep mountainous terrain. Ecological theory indicates that small isolated populations in disturbance-prone landscapes are at greatest risk of extirpation because immigration and recolonization are not possible. On the contrary, many above-barrier populations are currently thriving while their downstream counterparts are dwindling. This quandary led us to explore geomorphic knickpoints as a mechanism for disconnecting hillslope and channel processes by limiting channel incision and decreasing the pace of base-level lowering. Using LiDAR from the Oregon Coast Range, we found gentler channel gradients, wider valleys, lower gradient hillslopes, and less shallow landslide potential in an above-barrier catchment compared to a neighboring catchment devoid of persistent knickpoints. Based on this unique geomorphic template, above-barrier channel networks are less prone to debris flows and other episodic sediment fluxes. These above-barrier catchments also have greater resiliency to flooding, owing to wider valleys with greater floodplain connectivity. Habitat preference models further indicate that salmonid habitat is present in greater quantity and quality in these above-barrier networks. Therefore the paradox of the persistence of small isolated fish populations may be facilitated by a geomorphic mechanism that both limits their connectivity to larger fish populations yet dampens the effect of disturbance by decreasing connections between hillslope and channel processes above geomorphic knickpoints.

  16. Ichthyophonus-induced cardiac damage: a mechanism for reduced swimming stamina in salmonids

    USGS Publications Warehouse

    Kocan, R.; LaPatra, S.; Gregg, J.; Winton, J.; Hershberger, P.

    2006-01-01

    Swimming stamina, measured as time-to-fatigue, was reduced by approximately two-thirds in rainbow trout experimentally infected with Ichthyophonus. Intensity of Ichthyophonus infection was most severe in cardiac muscle but multiple organs were infected to a lesser extent. The mean heart weight of infected fish was 40% greater than that of uninfected fish, the result of parasite biomass, infiltration of immune cells and fibrotic (granuloma) tissue surrounding the parasite. Diminished swimming stamina is hypothesized to be due to cardiac failure resulting from the combination of parasite-damaged heart muscle and low myocardial oxygen supply during sustained aerobic exercise. Loss of stamina in Ichthyophonus-infected salmonids could explain the poor performance previously reported for wild Chinook and sockeye salmon stocks during their spawning migration. ?? 2006 Blackwell Publishing Ltd.

  17. A disturbance-based ecosystem approach to maintaining and restoring freshwater habitats of evolutionarily significant units of anadromous salmonids in the Pacific Northwest.

    Treesearch

    G.H. Reeves; L.E. Benda; K.M. Burnett; P.A. Bisson; J.R. Sedell

    1995-01-01

    To preserve and recover evolutionarily significant units (ESUs) of anadromous salmonids Oncorhynchus spp. in the Pacific Northwest, long-term and short-term ecological processes that create and maintain freshwater habitats must be restored and protected. Aquatic ecosystems through- out the region are dynamic in space and time, and lack of...

  18. Weirs: Counting and sampling adult salmonids in streams and rivers

    USGS Publications Warehouse

    Zimmerman, Christian E.; Zabkar, Laura M.; Johnson, David H.; Shrier, Brianna M.; O'Neal, Jennifer S.; Knutzen, John A.; Augerot, Xanthippe; O'Neal, Thomas A.; Pearsons, Todd N.

    2007-01-01

    Weirs—which function as porous barriers built across stream—have long been used to capture migrating fish in flowing waters. For example, the Netsilik peoples of northern Canada used V-shaped weirs constructed of river rocks gathered onsite to capture migrating Arctic char Salvelinus alpinus (Balikci 1970). Similarly, fences constructed of stakes and a latticework of willow branches or staves were used by Native Americans to capture migrating salmon in streams along the West Coast of North America (Stewart 1994). In modern times, weirs have also been used in terminal fisheries and to capture brood fish for use in fish culture. Weirs have been used to gather data on age structure, condition, sex ratio, spawning escapement, abundance, and migratory patterns of fish in streams. One of the critical elements of fisheries management and stock assessment of salmonids is a count of adult fish returning to spawn. Weirs are frequently used to capture or count fish to determine status and trends of populations or direct inseason management of fisheries; generally, weirs are the standard against which other techniques are measured. To evaluate fishery management actions, the number of fish escaping to spawn is often compared to river-specific target spawning requirements (O’Connell and Dempson 1995). A critical factor in these analyses is the determination of total run size (O’Connell 2003). O’Connell compared methods of run-size estimation against absolute counts from a rigid weir and concluded that, given the uncertainty of estimators, the absolute counts obtained at the weir wer significantly better than modeled estimates, which deviated as much as 50–60% from actual counts. The use of weirs is generally restricted to streams and small rivers because of construction expense, formation of navigation barriers, and the tendency of weirs to clog with debris, which can cause flooding and collapse of the structure (Hubert 1996). When feasible, however, weirs are

  19. Survival Estimates for the Passage of Juvenile Salmonids through Snake River Dams and Reservoirs, 1994 Annual Report.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Muir, William D.

    1995-02-01

    In 1994, the National Marine Fisheries Service and the University of Washington completed the second year of a multi-year study to estimate survival of juvenile salmonids (Oncorhynchus spp.) passing through the dams and reservoirs of the Snake River. Actively migrating smolts were collected at selected locations above, at, and below Lower Granite Dam, tagged with passive integrated transponder (PIT) tags, and released to continue their downstream migration. Survival estimates were calculated using the Single-Release, Modified Single-Release, and Paired-Release Models.

  20. Accurate aging of juvenile salmonids using fork lengths

    USGS Publications Warehouse

    Sethi, Suresh; Gerken, Jonathon; Ashline, Joshua

    2017-01-01

    Juvenile salmon life history strategies, survival, and habitat interactions may vary by age cohort. However, aging individual juvenile fish using scale reading is time consuming and can be error prone. Fork length data are routinely measured while sampling juvenile salmonids. We explore the performance of aging juvenile fish based solely on fork length data, using finite Gaussian mixture models to describe multimodal size distributions and estimate optimal age-discriminating length thresholds. Fork length-based ages are compared against a validation set of juvenile coho salmon, Oncorynchus kisutch, aged by scales. Results for juvenile coho salmon indicate greater than 95% accuracy can be achieved by aging fish using length thresholds estimated from mixture models. Highest accuracy is achieved when aged fish are compared to length thresholds generated from samples from the same drainage, time of year, and habitat type (lentic versus lotic), although relatively high aging accuracy can still be achieved when thresholds are extrapolated to fish from populations in different years or drainages. Fork length-based aging thresholds are applicable for taxa for which multiple age cohorts coexist sympatrically. Where applicable, the method of aging individual fish is relatively quick to implement and can avoid ager interpretation bias common in scale-based aging.

  1. Approach, passage, and survival of juvenile salmonids at Little Goose Dam, Washington: Post-construction evaluation of a temporary spillway weir, 2009

    USGS Publications Warehouse

    Beeman, J.W.; Braatz, A.C.; Hansel, H.C.; Fielding, S.D.; Haner, P.V.; Hansen, G.S.; Shurtleff, D.J.; Sprando, J.M.; Rondorf, D.W.

    2010-01-01

    This report describes a study of dam passage and survival of radio-tagged juvenile salmonids after installation of a temporary spillway weir (TSW) at Little Goose Dam, Washington, in 2009. The purpose of the study was to document fish passage and survival when the dam was operated with the TSW in place. Spillway weirs are one of several methods used to improve downstream passage of juvenile salmonids. Each spillway weir design is based on the concept of providing an overflow weir with a depth more similar to the natural migration depth of juvenile salmonids than conventional spill bays. Little Goose Dam was the last of the four lower Snake River dams to have a spillway weir installed. This was the first year that some form of surface passage device was operating at all Snake River and Columbia River dams between Lewiston, Idaho, and the Columbia River estuary. The study design stipulated that a total of 30 percent of the river discharge would continuously be passed over the TSW and the conventional spill bays, and this percentage was achieved. The TSW also was to be operated at the 'low crest' elevation during the spring and the 'high crest' elevation during the summer, but the TSW was only operated at the low crest elevation during this study. Behavior, passage, and survival of spring and summer juvenile salmonid migrants passing through Little Goose Dam were examined using radio telemetry. Survival was estimated using the Route Specific Survival Model (RSSM) by releasing tagged fish near Central Ferry State Park 21 kilometers upstream of the dam and in the tailrace approximately 0.5 kilometer downstream of the dam. From April 18 to May 21, 2009, 1,520 yearling Chinook salmon (Oncorhynchus tshawytscha) and 1,517 juvenile steelhead (O. mykiss) were radio tagged and released. From June 6 to July 5, 2009, 4,251 subyearling Chinook salmon (O. tshawytscha) were radio tagged and released. Release dates of subyearling Chinook salmon were selected to avoid 'reservoir

  2. Survival and migration behavior of juvenile salmonids at Lower Granite Dam, 2006

    USGS Publications Warehouse

    Beeman, John W.; Fielding, Scott D.; Braatz, Amy C.; Wilkerson, Tamara S.; Pope, Adam C.; Walker, Christopher E.; Hardiman, Jill M.; Perry, Russell W.; Counihan, Timothy D.

    2008-01-01

    We described behavior and estimated passage and survival parameters of juvenile salmonids during spring and summer migration periods at Lower Granite Dam in 2006. During the spring, the study was designed to examine the effects of the Behavioral Guidance Structure (BGS) by using a randomized-block BGS Stored / BGS Deployed treatment design. The summer study was designed to compare passage and survival through Lower Granite Dam using a randomized-block design during two spill treatments while the BGS was in the stored position. We used the Route Specific Survival Model to estimate survival and passage probabilities of hatchery yearling Chinook salmon, hatchery juvenile steelhead, and hatchery and wild subyearling Chinook salmon. We also estimated fish guidance efficiency (FGE), fish passage efficiency (FPE), Removable Spillway Weir passage effectiveness (RPE), spill passage effectiveness (SPY), and combined spill and RSW passage effectiveness.

  3. Demographics of piscivorous colonial waterbirds and management implications for ESA-listed salmonids on the Columbia Plateau

    USGS Publications Warehouse

    Adkins, Jessica Y.; Lyons, Donald E.; Loschl, Peter J.; Roby, Daniel D.; Collis, Ken; Evans, Allen F.; Hostetter, Nathan J.

    2014-01-01

    We investigated colony size, productivity, and limiting factors for five piscivorous waterbird species nesting at 18 locations on the Columbia Plateau (Washington) during 2004–2010 with emphasis on species with a history of salmonid (Oncorhynchus spp.) depredation. Numbers of nesting Caspian terns (Hydroprogne caspia) and double-crested cormorants (Phalacrocorax auritus) were stable at about 700–1,000 breeding pairs at five colonies and about 1,200–1,500 breeding pairs at four colonies, respectively. Numbers of American white pelicans (Pelecanus erythrorhynchos) increased at Badger Island, the sole breeding colony for the species on the Columbia Plateau, from about 900 individuals in 2007 to over 2,000 individuals in 2010. Overall numbers of breeding California gulls (Larus californicus) and ring-billed gulls (L. delawarensis) declined during the study, mostly because of the abandonment of a large colony in the mid-Columbia River. Three gull colonies below the confluence of the Snake and Columbia rivers increased substantially, however. Factors that may limit colony size and productivity for piscivorous waterbirds nesting on the Columbia Plateau included availability of suitable nesting habitat, interspecific competition for nest sites, predation, gull kleptoparasitism, food availability, and human disturbance. Based on observed population trends alone, there is little reason to project increased impacts to juvenile salmonid survival from tern and cormorant populations. Additional monitoring and evaluation may be warranted to assess future impacts of the growing Badger Island American white pelican colony and those gull colonies located near mainstem dams or associated with Caspian tern colonies where kleptoparasitism is common.

  4. Morphology of certain viruses of Salmonid Fishes. I. in vitro studies of some viruses causing Hematopoietic Necrosis

    USGS Publications Warehouse

    Amend, Donald F.; Chambers, Velma C.

    1970-01-01

    An electron microscope study was performed on three virus isolates that caused hematopoietic necrosis in salmonid fishes: infectious hematopoietic necrosis (IHN), Oregon Sockeye Disease (OSD), and Sacramento River Chinook Salmon Disease (SRCD). All three isolates were examined by negative staining of fathead minnow (FHM) monolayer tissue culture concentrates and IHN virus was also examined in thin sections of FHM cells. Viruslike particles were observed in infected tissues, but similar structures were not found in uninfected cultures. All three isolates were bullet-shaped, but oval and truncated forms were also observed. Mean measurements of particles from IHN-virus-infected tissue were 158 × 90 mμ. They consisted of an outer coat 15 mμ thick, a core 60 mμ in diameter, subunits about 5 mμ, and an axial pore about 20 mμ in diameter. These particles also were seen budding from the cytoplasmic membrane. Similar particles from SRCD were 159 × 90 mμ and isolates from OSD were 181 × 91 mμ. The three isolates were morphologically indistinguishable from one another and the greater length of OSD was considered insignificant. IHN, SRCD, and OSD viruses were tentatively placed in the rhabdovirus group, but serological studies are needed to determine if they are antigenically identical or should be included as separate members. Biochemical and physical characteristics of these viruses and a comparison with other salmonid viruses is also discussed.

  5. Oral vaccination of Atlantic salmon (Salmo salar) against salmonid rickettsial septicaemia.

    PubMed

    Tobar, Jaime A; Jerez, Sofía; Caruffo, Mario; Bravo, Catalina; Contreras, Francisco; Bucarey, Sergio A; Harel, Moti

    2011-03-09

    Effective oral immunization systems may be very helpful to the salmon industry, particularly during the seawater growth stages in which vaccination through injection is not possible. During the seawater growing stage, fish become more susceptible to several types of disease, due to the natural decay of vaccine-induced immune responses. In this study, we demonstrate the immune response and efficacy of a new salmonid rickettsial septicaemia (SRS) oral vaccine, developed using MicroMatrix™ Technology. The vaccine, which is administered together with daily feed ration, induces a specific immune response at local and systemic levels. Anti-Piscirickettsia salmonis specific antibodies were detected as soon as 300 degree-days after vaccination. Furthermore, oral vaccination was able to protect fish against a lethal pathogen challenge when administered either as a primary vaccination or as a booster for an injected vaccine. Results show that oral vaccination is an efficacious treatment for the prevention of SRS outbreaks throughout the salmon culture period. Copyright © 2010 Elsevier Ltd. All rights reserved.

  6. A simple model that identifies potential effects of sea-level rise on estuarine and estuary-ecotone habitat locations for salmonids in Oregon, USA

    Treesearch

    Rebecca Flitcroft; Kelly Burnett; Kelly Christiansen

    2013-01-01

    Diadromous aquatic species that cross a diverse range of habitats (including marine, estuarine, and freshwater) face different effects of climate change in each environment. One such group of species is the anadromous Pacific salmon (Oncorhynchus spp.). Studies of the potential effects of climate change on salmonids have focused on both marine and...

  7. The potential influence of changing climate on the persistence of salmonids of the inland west

    USGS Publications Warehouse

    Haak, A.L.; Williams, J.E.; Isaak, D.; Todd, A.; Muhlfeld, C.C.; Kershner, J.L.; Gresswell, R.E.; Hostetler, S.W.; Neville, H.M.

    2010-01-01

    The Earth's climate warmed steadily during the 20th century, and mean annual air temperatures are estimated to have increased by 0.6°C (Intergovernmental Panel on Climate Change, 2007). Although many cycles of warming and cooling have occurred in the past, the most recent warming period is unique in its rate and magnitude of change (Siegenthaler and others, 2005) and in its association with anthropogenic emissions of greenhouse gases (Intergovernmental Panel on Climate Change , 2007). The climate in the western United States warmed in concert with the global trend but at an accelerated rate (+0.8°C during the 20th century; Saunders and others, 2008). The region could also prove especially sensitive to future changes because the relatively small human population is growing rapidly, as are demands on limited water supplies. Regional hydrological patterns are dominated by seasonal snow accumulation at upper elevations. Most of the region is relatively dry, and both terrestrial and aquatic ecosystems are strongly constrained b y water availability (Barnett and others, 2008; Brown and others, 2008). Stream environments are dynamic and climatically extreme, and salmonid fishes are the dominant elements of the native biodiversity (McPhail and Lindsey, 1986; Waples and others, 2008). Salmonids have broad economic and ecologic importance, but a century of intensive water resource development, nonnative fish stocking, and land use has significantly reduced many populations and several taxa are now protected under the Endangered Species Act (Thurow and others, 1997; Trotter, 2008). Because salmonids require relatively pristine, cold water environments and are often isolated in headwater habitats, members of this group may be especially vulnerable to the effects of a warming climate (Keleher and Rahel, 1996; Rieman and others, 2007; Williams and others, 2009). Warming during the 20th century drove a series of environmental trends that have profound implications for many

  8. Erection of Ceratonova n. gen. (Myxosporea: Ceratomyxidae) to encompass freshwater species C. gasterostea n. sp. from threespine stickleback (Gasterosteus aculeatus) and C. shasta n. comb. from salmonid fishes.

    PubMed

    Atkinson, S D; Foott, J S; Bartholomew, J L

    2014-10-01

    Ceratonova gasterostea n. gen. n. sp. is described from the intestine of freshwater Gasterosteus aculeatus L. from the Klamath River, California. Myxospores are arcuate, 22.4 ± 2.6 μm thick, 5.2 ± 0.4 μm long, posterior angle 45° ± 24°, with 2 sub-spherical polar capsules, diameter 2.3 ± 0.2 μm, which lie adjacent to the suture. Its ribosomal small subunit sequence was most similar to an intestinal parasite of salmonid fishes, Ceratomyxa shasta (97%, 1,671/1,692 nucleotides), and distinct from all other Ceratomyxa species (<85%), which are typically coelozoic parasites in the gall bladder or urinary system of marine fishes. We propose erection of genus Ceratonova to contain both intestinal, freshwater species and reassign the salmonid parasite as Ceratonova shasta n. comb.

  9. Clock genes and their genomic distributions in three species of salmonid fishes: Associations with genes regulating sexual maturation and cell cycling

    PubMed Central

    2010-01-01

    Background Clock family genes encode transcription factors that regulate clock-controlled genes and thus regulate many physiological mechanisms/processes in a circadian fashion. Clock1 duplicates and copies of Clock3 and NPAS2-like genes were partially characterized (genomic sequencing) and mapped using family-based indels/SNPs in rainbow trout (RT)(Oncorhynchus mykiss), Arctic charr (AC)(Salvelinus alpinus), and Atlantic salmon (AS)(Salmo salar) mapping panels. Results Clock1 duplicates mapped to linkage groups RT-8/-24, AC-16/-13 and AS-2/-18. Clock3/NPAS2-like genes mapped to RT-9/-20, AC-20/-43, and AS-5. Most of these linkage group regions containing the Clock gene duplicates were derived from the most recent 4R whole genome duplication event specific to the salmonids. These linkage groups contain quantitative trait loci (QTL) for life history and growth traits (i.e., reproduction and cell cycling). Comparative synteny analyses with other model teleost species reveal a high degree of conservation for genes in these chromosomal regions suggesting that functionally related or co-regulated genes are clustered in syntenic blocks. For example, anti-müllerian hormone (amh), regulating sexual maturation, and ornithine decarboxylase antizymes (oaz1 and oaz2), regulating cell cycling, are contained within these syntenic blocks. Conclusions Synteny analyses indicate that regions homologous to major life-history QTL regions in salmonids contain many candidate genes that are likely to influence reproduction and cell cycling. The order of these genes is highly conserved across the vertebrate species examined, and as such, these genes may make up a functional cluster of genes that are likely co-regulated. CLOCK, as a transcription factor, is found within this block and therefore has the potential to cis-regulate the processes influenced by these genes. Additionally, clock-controlled genes (CCGs) are located in other life-history QTL regions within salmonids suggesting that

  10. Single nucleotide polymorphism (SNP) discovery in duplicated genomes: intron-primed exon-crossing (IPEC) as a strategy for avoiding amplification of duplicated loci in Atlantic salmon (Salmo salar) and other salmonid fishes

    PubMed Central

    Ryynänen, Heikki J; Primmer, Craig R

    2006-01-01

    Background Single nucleotide polymorphisms (SNPs) represent the most abundant type of DNA variation in the vertebrate genome, and their applications as genetic markers in numerous studies of molecular ecology and conservation of natural populations are emerging. Recent large-scale sequencing projects in several fish species have provided a vast amount of data in public databases, which can be utilized in novel SNP discovery in salmonids. However, the suggested duplicated nature of the salmonid genome may hamper SNP characterization if the primers designed in conserved gene regions amplify multiple loci. Results Here we introduce a new intron-primed exon-crossing (IPEC) method in an attempt to overcome this duplication problem, and also evaluate different priming methods for SNP discovery in Atlantic salmon (Salmo salar) and other salmonids. A total of 69 loci with differing priming strategies were screened in S. salar, and 27 of these produced ~13 kb of high-quality sequence data consisting of 19 SNPs or indels (one per 680 bp). The SNP frequency and the overall nucleotide diversity (3.99 × 10-4) in S. salar was lower than reported in a majority of other organisms, which may suggest a relative young population history for Atlantic salmon. A subset of primers used in cross-species analyses revealed considerable variation in the SNP frequencies and nucleotide diversities in other salmonids. Conclusion Sequencing success was significantly higher with the new IPEC primers; thus the total number of loci to screen in order to identify one potential polymorphic site was six times less with this new strategy. Given that duplication may hamper SNP discovery in some species, the IPEC method reported here is an alternative way of identifying novel polymorphisms in such cases. PMID:16872523

  11. Turbulence investigation and reproduction for assisting downstream migrating juvenile salmonids, Part II of II: Effects of induced turbulence on behavior of juvenile salmon, 2001-2005 final report

    USGS Publications Warehouse

    Perry, R.; Farley , M.; Hansen, G.; Morse , J.; Rondorf, D.

    2005-01-01

    Passage through dams is a major source of mortality of anadromous juvenile salmonids because some populations must negotiate up to eight dams in Columbia and Snake rivers. Dams cause direct mortality when fish pass through turbines, but dams may also cause indirect mortality by altering migration conditions in rivers. Forebays immediately upstream of dams have decreased the water velocity of rivers and may contribute substantially to the total migration delay of juvenile salmonids. Recently, Coutant (2001a) suggested that in addition to low water velocities, lack of natural turbulence may contribute to migration delay by causing fish to lose directional cues. Coutant (2001a) further hypothesized that restoring turbulence in dam forebays may reduce migration delay by providing directional cues that allow fish to find passage routes more quickly (Coutant 2001a). Although field experiments have yielded proof of the concept of using induced turbulence to guide fish to safe passage routes, little is known about mechanisms actually causing behavioral changes. To test hypotheses about how turbulence influences movement and behavior of migrating juvenile salmonids, we conducted two types of controlled experiments at Cowlitz Falls Dam, Washington. A common measure of migration delay is the elapsed time between arrival at, and passage through, a dam. Therefore, for the first set of experiments, we tested the effect of induced turbulence on the elapsed time needed for fish to traverse through a raceway and pass over a weir at its downstream end (time trial experiment). If turbulence helps guide fish to passage routes, then fish should pass through the raceway quicker in the presence of appropriately scaled and directed turbulent cues. Second, little is known about how the physical properties of water movement provide directional cues to migrating juvenile salmonids. To examine the feasibility of guiding fish with turbulence, we tested whether directed turbulence could guide

  12. Three-spined stickleback Gasterosteus aculeatus, as a possible paratenic host for salmonid nematodes in a subarctic lake.

    PubMed

    Braicovich, Paola E; Kuhn, Jesper A; Amundsen, Per-Arne; Marcogliese, David J

    2016-03-01

    In Takvatn, a subarctic lake in northern Norway, 35 of 162 three-spined sticklebacks examined were infected with 106 specimens of third-stage larvae of Philonema oncorhynchi. The prevalence and mean intensity of P. oncorhynchi were 10 % and 2.0 in 2013 and 24 % and 3.0 in 2014, respectively. A single specimen of Cystidicola farionis was found in an additional sample. While the latter is considered an accidental infection, three-spined sticklebacks may function as paratenic hosts of P. oncorhynchi, potentially enhancing its transmission to salmonids due to their central role in the lacustrine food web of this subarctic lake.

  13. A survey of the distribution of the PKD-parasite Tetracapsuloides bryosalmonae (Cnidaria: Myxozoa: Malacosporea) in salmonids in Norwegian rivers - additional information gleaned from formerly collected fish.

    PubMed

    Mo, T A; Jørgensen, A

    2017-05-01

    The malacosporean Tetracapsuloides bryosalmonae was detected in kidneys from Atlantic salmon parr in 64 of 91 sampled Norwegian rivers. Using real-time PCR, this parasite was found to be present in Atlantic salmon parr in rivers along the whole coast, from the northernmost and southernmost areas of the country. In addition, T. bryosalmonae was found in kidneys from brown trout parr in 17 of 19 sampled rivers in south-east Norway, and in Arctic charr sampled in the River Risfjordelva, located at the northernmost edge of the European mainland. In conclusion, T. bryosalmonae has a widespread distribution in salmonids in Norwegian watercourses. Proliferative kidney disease (PKD) caused by T. bryosalmonae and PKD-induced mortality has been observed in salmonids in several Norwegian rivers and it can be speculated that more PKD outbreaks will occur as a result of climate change. © 2016 The Authors. Journal of Fish Diseases Published by John Wiley & Sons Ltd.

  14. Initial Detection and Molecular Characterization of Namaycush Herpesvirus (Salmonid Herpesvirus 5) in Lake Trout.

    PubMed

    Glenney, Gavin W; Barbash, Patricia A; Coll, John A

    2016-03-01

    A novel herpesvirus was found by molecular methods in samples of Lake Trout Salvelinus namaycush from Lake Erie, Pennsylvania, and Lake Ontario, Keuka Lake, and Lake Otsego, New York. Based on PCR amplification and partial sequencing of polymerase, terminase, and glycoprotein genes, a number of isolates were identified as a novel virus, which we have named Namaycush herpesvirus (NamHV) salmonid herpesvirus 5 (SalHV5). Phylogenetic analyses of three NamHV genes indicated strong clustering with other members of the genus Salmonivirus, placing these isolates into family Alloherpesviridae. The NamHV isolates were identical in the three partially sequenced genes; however, they varied from other salmonid herpesviruses in nucleotide sequence identity. In all three of the genes sequenced, NamHV shared the highest sequence identity with Atlantic Salmon papillomatosis virus (ASPV; SalHV4) isolated from Atlantic Salmon Salmo salar in northern Europe, including northwestern Russia. These results lead one to believe that NamHV and ASPV have a common ancestor that may have made a relatively recent host jump from Atlantic Salmon to Lake Trout or vice versa. Partial nucleotide sequence comparisons between NamHV and ASPV for the polymerase and glycoprotein genes differ by >5% and >10%, respectively. Additional nucleotide sequence comparisons between NamHV and epizootic epitheliotropic disease virus (EEDV/SalHV3) in the terminase, glycoprotein, and polymerase genes differ by >5%, >20%, and >10%, respectively. Thus, NamHV and EEDV may be occupying discrete ecological niches in Lake Trout. Even though NamHV shared the highest genetic identity with ASPV, each of these viruses has a separate host species, which also implies speciation. Additionally, NamHV has been detected over the last 4 years in four separate water bodies across two states, which suggests that NamHV is a distinct, naturally replicating lineage. This, in combination with a divergence in nucleotide sequence from EEDV

  15. Ion composition and temperature in the topside ionosphere.

    NASA Technical Reports Server (NTRS)

    Brace, L. H.; Dunham, G. S.; Mayr, H. G.

    1967-01-01

    Particle and energy continuity equations derived and solved by computer method ion composition and plasma temperature measured by Explorer XXII PARTICLE and energy continuity equations derived and solved by computer method for ion composition and plasma temperature measured by Explorer XXII

  16. Assessing survival of Mid-Columbia River released juvenile salmonids at McNary Dam, Washington, 2008-09

    USGS Publications Warehouse

    Evans, Scott D.; Walker, Christopher E.; Brewer, Scott J.; Adams, Noah S.

    2010-01-01

    Few studies have evaluated survival of juvenile salmon over long river reaches in the Columbia River and information regarding the survival of sockeye salmon at lower Columbia River dams is lacking. To address these information gaps, the U.S. Geological Survey was contracted by the U.S. Army Corps of Engineers to evaluate the possibility of using tagged fish released in the Mid-Columbia River to assess passage and survival at and downstream of McNary Dam. Using the acoustic telemetry systems already in place for a passage and survival study at McNary Dam, fish released from the tailraces of Wells, Rocky Reach, Rock Island, Wanapum, and Priest Rapids Dams were detected at McNary Dam and at the subsequent downstream arrays. These data were used to generate route-specific survival probabilities using single-release models from fish released in the Mid-Columbia River. We document trends in passage and survival probabilities at McNary Dam for yearling Chinook and sockeye salmon and juvenile steelhead released during studies in the Mid-Columbia River. Trends in the survival and passage of these juvenile salmonid species are presented and discussed. However, comparisons made across years and between study groups are not possible because of differences in the source of the test fish, the type of acoustic tags used, the absence of the use of passive integrated transponder tags in some of the release groups, differences in tagging and release protocols, annual differences in dam operations and configurations, differences in how the survival models were constructed (that is, number of routes that could be estimated given the number of fish detected), and the number and length of reaches included in the analysis (downstream reach length and arrays). Despite these differences, the data we present offer a unique opportunity to examine the migration behavior and survival of a group of fish that otherwise would not be studied. This is particularly true for sockeye salmon because

  17. Functional response and capture timing in an individual-based model: predation by northern squawfish (Ptychocheilus oregonensis) on juvenile salmonids in the Columbia River

    USGS Publications Warehouse

    Petersen, James H.; DeAngelis, Donald L.

    1992-01-01

    The behavior of individual northern squawfish (Ptychocheilus oregonensis) preying on juvenile salmonids was modeled to address questions about capture rate and the timing of prey captures (random versus contagious). Prey density, predator weight, prey weight, temperature, and diel feeding pattern were first incorporated into predation equations analogous to Holling Type 2 and Type 3 functional response models. Type 2 and Type 3 equations fit field data from the Columbia River equally well, and both models predicted predation rates on five of seven independent dates. Selecting a functional response type may be complicated by variable predation rates, analytical methods, and assumptions of the model equations. Using the Type 2 functional response, random versus contagious timing of prey capture was tested using two related models. ln the simpler model, salmon captures were assumed to be controlled by a Poisson renewal process; in the second model, several salmon captures were assumed to occur during brief "feeding bouts", modeled with a compound Poisson process. Salmon captures by individual northern squawfish were clustered through time, rather than random, based on comparison of model simulations and field data. The contagious-feeding result suggests that salmonids may be encountered as patches or schools in the river.

  18. Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole-genome duplication.

    PubMed

    Carmona-Antoñanzas, Greta; Zheng, Xiaozhong; Tocher, Douglas R; Leaver, Michael J

    2016-10-10

    Fatty acyl elongase 5 (elovl5) is a critical enzyme in the vertebrate biosynthetic pathway which produces the physiologically essential long-chain polyunsaturated fatty acids (LC-PUFA), docosahexenoic acid (DHA), and eicosapentenoic acid (EPA) from 18 carbon fatty acids precursors. In contrast to most other vertebrates, Atlantic salmon possess two copies of elovl5 (elovl5a and elovl5b) as a result of a whole-genome duplication (WGD) which occurred at the base of the salmonid lineage. WGDs have had a major influence on vertebrate evolution, providing extra genetic material, enabling neofunctionalization to accelerate adaptation and speciation. However, little is known about the mechanisms by which such duplicated homeologous genes diverge. Here we show that homeologous Atlantic salmon elovl5a and elovl5b genes have been asymmetrically colonised by transposon-like elements. Identical locations and identities of insertions are also present in the rainbow trout duplicate elovl5 genes, but not in the nearest extant representative preduplicated teleost, the northern pike. Both elovl5 salmon duplicates possessed conserved regulatory elements that promoted Srebp1- and Srebp2-dependent transcription, and differences in the magnitude of Srebp response between promoters could be attributed to a tandem duplication of SRE and NF-Y cofactor binding sites in elovl5b. Furthermore, an insertion in the promoter region of elovl5a confers responsiveness to Lxr/Rxr transcriptional activation. Our results indicate that most, but not all, transposon mobilisation into elovl5 genes occurred after the split from the common ancestor of pike and salmon, but before more recent salmonid speciations, and that divergence of elovl5 regulatory regions have enabled neofuntionalization by promoting differential expression of these homeologous genes. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. A comparative examination of cortisol effects on muscle myostatin and HSP90 gene expression in salmonids.

    PubMed

    Galt, Nicholas J; McCormick, Stephen D; Froehlich, Jacob Michael; Biga, Peggy R

    2016-10-01

    Cortisol, the primary corticosteroid in teleost fishes, is released in response to stressors to elicit local functions, however little is understood regarding muscle-specific responses to cortisol in these fishes. In mammals, glucocorticoids strongly regulate the muscle growth inhibitor, myostatin, via glucocorticoid response elements (GREs) leading to muscle atrophy. Bioinformatics methods suggest that this regulatory mechanism is conserved among vertebrates, however recent evidence suggests some fishes exhibit divergent regulation. Therefore, the aim of this study was to evaluate the conserved actions of cortisol on myostatin and hsp90 expression to determine if variations in cortisol interactions have emerged in salmonid species. Representative salmonids; Chinook salmon (Oncorhynchus tshawytscha), cutthroat trout (Oncorhynchus clarki), brook trout (Salvelinus fontinalis), and Atlantic salmon (Salmo salar); were injected intraperitoneally with a cortisol implant (50μg/g body weight) and muscle gene expression was quantified after 48h. Plasma glucose and cortisol levels were significantly elevated by cortisol in all species, demonstrating physiological effectiveness of the treatment. HSP90 mRNA levels were elevated by cortisol in brook trout, Chinook salmon, and Atlantic salmon, but were decreased in cutthroat trout. Myostatin mRNA levels were affected in a species, tissue (muscle type), and paralog specific manner. Cortisol treatment increased myostatin expression in brook trout (Salvelinus) and Atlantic salmon (Salmo), but not in Chinook salmon (Oncorhynchus) or cutthroat trout (Oncorhynchus). Interestingly, the VC alone increased myostatin mRNA expression in Chinook and Atlantic salmon, while the addition of cortisol blocked the response. Taken together, these results suggest that cortisol affects muscle-specific gene expression in species-specific manners, with unique Oncorhynchus-specific divergence observed, that are not predictive solely based upon

  20. Piscine reovirus: Genomic and molecular phylogenetic analysis from farmed and wild salmonids collected on the Canada/US Pacific Coast

    USGS Publications Warehouse

    Siah, Ahmed; Morrison, Diane B.; Fringuelli, Elena; Savage, Paul S.; Richmond, Zina; Purcell, Maureen K.; Johns, Robert; Johnson, Stewart C.; Sakasida, Sonja M.

    2015-01-01

    Piscine reovirus (PRV) is a double stranded non-enveloped RNA virus detected in farmed and wild salmonids. This study examined the phylogenetic relationships among different PRV sequence types present in samples from salmonids in Western Canada and the US, including Alaska (US), British Columbia (Canada) and Washington State (US). Tissues testing positive for PRV were partially sequenced for segment S1, producing 71 sequences that grouped into 10 unique sequence types. Sequence analysis revealed no identifiable geographical or temporal variation among the sequence types. Identical sequence types were found in fish sampled in 2001, 2005 and 2014. In addition, PRV positive samples from fish derived from Alaska, British Columbia and Washington State share identical sequence types. Comparative analysis of the phylogenetic tree indicated that Canada/US Pacific Northwest sequences formed a subgroup with some Norwegian sequence types (group II), distinct from other Norwegian and Chilean sequences (groups I, III and IV). Representative PRV positive samples from farmed and wild fish in British Columbia and Washington State were subjected to genome sequencing using next generation sequencing methods. Individual analysis of each of the 10 partial segments indicated that the Canadian and US PRV sequence types clustered separately from available whole genome sequences of some Norwegian and Chilean sequences for all segments except the segment S4. In summary, PRV was genetically homogenous over a large geographic distance (Alaska to Washington State), and the sequence types were relatively stable over a 13 year period.

  1. Piscine Reovirus: Genomic and Molecular Phylogenetic Analysis from Farmed and Wild Salmonids Collected on the Canada/US Pacific Coast

    PubMed Central

    Siah, Ahmed; Morrison, Diane B.; Fringuelli, Elena; Savage, Paul; Richmond, Zina; Johns, Robert; Purcell, Maureen K.; Johnson, Stewart C.; Saksida, Sonja M.

    2015-01-01

    Piscine reovirus (PRV) is a double stranded non-enveloped RNA virus detected in farmed and wild salmonids. This study examined the phylogenetic relationships among different PRV sequence types present in samples from salmonids in Western Canada and the US, including Alaska (US), British Columbia (Canada) and Washington State (US). Tissues testing positive for PRV were partially sequenced for segment S1, producing 71 sequences that grouped into 10 unique sequence types. Sequence analysis revealed no identifiable geographical or temporal variation among the sequence types. Identical sequence types were found in fish sampled in 2001, 2005 and 2014. In addition, PRV positive samples from fish derived from Alaska, British Columbia and Washington State share identical sequence types. Comparative analysis of the phylogenetic tree indicated that Canada/US Pacific Northwest sequences formed a subgroup with some Norwegian sequence types (group II), distinct from other Norwegian and Chilean sequences (groups I, III and IV). Representative PRV positive samples from farmed and wild fish in British Columbia and Washington State were subjected to genome sequencing using next generation sequencing methods. Individual analysis of each of the 10 partial segments indicated that the Canadian and US PRV sequence types clustered separately from available whole genome sequences of some Norwegian and Chilean sequences for all segments except the segment S4. In summary, PRV was genetically homogenous over a large geographic distance (Alaska to Washington State), and the sequence types were relatively stable over a 13 year period. PMID:26536673

  2. Rates of consumption of juvenile salmonids and alternative pray fish by northern squawfish, walleyes, smallmouth bass, and channel catfish in John Day Reservoir, Columbia River

    USGS Publications Warehouse

    Vigg, Steven; Poe, Thomas P.; Prendergast , Linda A.; Hansel, Hal C.

    1991-01-01

    Adult northern squawfish Ptychocheilus oregonensis, walleyes Stizostedion vitreum, smallmouth bass Micropterus dolomieu, and channel catfish Ictalurus punctatus were sampled from four regions of John Day Reservoir from April to August 1983–1986 to quantify their consumption of 13 species of prey fish, particularly seaward-migrating juvenile Pacific salmon and steelhead (Oncorhynchus spp.). Consumption rates were estimated from field data on stomach contents and digestion rate relations determined in previous investigations. For each predator, consumption rates varied by reservoir area, month, time of day, and predator size or age. The greatest daily consumption of salmonids by northern squawfish and channel catfish (0.7 and 0.5 prey/predator) occurred in the upper end of the reservoir below McNary Dam. Greatest daily predation by walleyes (0.2 prey/predator) and smallmouth bass (0.04) occurred in the middle and lower reservoir. Consumption rates of all predators were highest in July, concurrent with maximum temperature and abundance of juvenile salmonids. Feeding by the predators tended to peak after dawn (0600–1200 hours) and near midnight (2000–2400). Northern squawfish below McNary Dam exhibited this pattern, but fed mainly in the morning hours down-reservoir. The daily ration of total prey fish was highest for northern squawfish over 451 mm fork length (> 13.2 mg/g predator), for walleyes 201–250 mm (42.5 mg/g), for smallmouth bass 176–200 mm (30.4 mg/g), and for channel catfish 401–450 mm (17.1 mg/g). Averaged over all predator sizes and sampling months (April–August), the total daily ration (fish plus other prey) of smallmouth bass (28.7 mg/ g) was about twice that of channel catfish (12.6), northern squawfish (14.1), and walleyes (14.2). However, northern squawfish was clearly the major predator on juvenile salmonids.

  3. Significance of Selective Predation and Development of Prey Protection Measures for Juvenile Salmonids in the Columbia and Snake River Reservoirs: Annual Progress Report, February 1993-February 1994.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Poe, Thomas P.

    1994-08-01

    This report addresses the problem of predator-prey interactions of juvenile salmonids in the Columbia and Snake River. Six papers are included on selective predation and prey protection. Attention is focused on monitoring the movements, the distribution, and the behavior of juvenile chinook salmon and northern squawfish.

  4. A rapid solid-phase extraction fluorometric method for thiamine and riboflavin in salmonid eggs

    USGS Publications Warehouse

    Zajicek, James L.; Tillitt, Donald E.; Brown, Scott B.; Brown, Lisa R.; Honeyfield, Dale C.; Fitzsimons, John D.

    2005-01-01

    A new method has been developed and successfully applied to the selective measurement of thiamine (nonphosphorylated), total thiamine (sum of thiamine, thiamine monophosphate [TMP], thiamine diphosphate [TDP], and thiamine triphosphate [TTP]), and potentially interfering riboflavin in acidic (2% trichloroacetic acid) extracts of selected salmonid and walleye egg samples. Acidic extracts of eggs were applied directly to end-capped C18, reversed-phase solid-phase extraction (SPE) columns and separated into three fractions by elution with mixtures of PO4 buffer (pH 2), methanol (10%), and acetonitrile (20%). All thiamine compounds recovered in the first two fractions were oxidized to their corresponding thiochromes with alkaline potassium hexacyanoferrate, and we measured the thiochrome fluorescence (excitation at 360 nm, emission at 460 nm) in a 96-well microplate reader. Riboflavin, recovered in third fraction (eluted with pH 2, 20% acetonitrile), was analyzed directly by measuring the fluorescence of this fraction (excitation at 450 nm, emission at 530 nm). Significant portions of the phosphate esters of thiamine (TMP, TDP, and presumably TTP), when present at low concentrations (< 10 nmol of total -thiamine per gram of egg), were not retained by the 100-mg SPE column, and were collected directly during sample loading and in a subsequent phosphoric acid rinse as fraction 1. Free thiamine (nonphosphorylated) and remaining portions of the TDP and TMP were then eluted in the second fraction with 10% methanol/PO4 buffer, whereas the un-ionized, relatively nonpolar riboflavin was eluted in the third fraction with 20% acetonitrile. This new method uses a traditional sample homogenization of egg tissue to extract thiamine compounds into 2% trichlororacetic acid solution; an inexpensive, commercially available SPE column; small amounts of sample (0.5-1 g); microliter volumes of solvents per sample; a traditional, relatively nonhazardous, oxidation of thiamine compounds to

  5. Year-Round Monitoring of Contaminants in Neal and Rogers Creeks, Hood River Basin, Oregon, 2011-12, and Assessment of Risks to Salmonids.

    PubMed

    Hapke, Whitney B; Morace, Jennifer L; Nilsen, Elena B; Alvarez, David A; Masterson, Kevin

    2016-01-01

    Pesticide presence in streams is a potential threat to Endangered Species Act listed salmonids in the Hood River basin, Oregon, a primarily forested and agricultural basin. Two types of passive samplers, polar organic chemical integrative samplers (POCIS) and semipermeable membrane devices (SPMDs), were simultaneously deployed at four sites in the basin during Mar. 2011-Mar. 2012 to measure the presence of pesticides, polybrominated diphenyl ethers (PBDEs), and polychlorinated biphenyls (PCBs). The year-round use of passive samplers is a novel approach and offers several new insights. Currently used pesticides and legacy contaminants, including many chlorinated pesticides and PBDEs, were present throughout the year in the basin's streams. PCBs were not detected. Time-weighted average water concentrations for the 2-month deployment periods were estimated from concentrations of chemicals measured in the passive samplers. Currently used pesticide concentrations peaked during spring and were detected beyond their seasons of expected use. Summed concentrations of legacy contaminants in Neal Creek were highest during July-Sept., the period with the lowest streamflows. Endosulfan was the only pesticide detected in passive samplers at concentrations exceeding Oregon or U.S. Environmental Protection Agency water-quality thresholds. A Sensitive Pesticide Toxicity Index (SPTI) was used to estimate the relative acute potential toxicity among sample mixtures. The acute potential toxicity of the detected mixtures was likely greater for invertebrates than for fish and for all samples in Neal Creek compared to Rogers Creek, but the indices appear to be low overall (<0.1). Endosulfans and pyrethroid insecticides were the largest contributors to the SPTIs for both sites. SPTIs of some discrete (grab) samples from the basin that were used for comparison exceeded 0.1 when some insecticides (azinphos methyl, chlorpyrifos, malathion) were detected at concentrations near or exceeding

  6. Year-round monitoring of contaminants in Neal and Rogers Creeks, Hood River Basin, Oregon, 2011-12, and assessment of risks to salmonids

    USGS Publications Warehouse

    Temple, Whitney B.; Morace, Jennifer L.; Nilsen, Elena B.; Alvarez, David; Masterson, Kevin

    2016-01-01

    Pesticide presence in streams is a potential threat to Endangered Species Act listed salmonids in the Hood River basin, Oregon, a primarily forested and agricultural basin. Two types of passive samplers, polar organic chemical integrative samplers (POCIS) and semipermeable membrane devices (SPMDs), were simultaneously deployed at four sites in the basin during Mar. 2011–Mar. 2012 to measure the presence of pesticides, polybrominated diphenyl ethers (PBDEs), and polychlorinated biphenyls (PCBs). The year-round use of passive samplers is a novel approach and offers several new insights. Currently used pesticides and legacy contaminants, including many chlorinated pesticides and PBDEs, were present throughout the year in the basin’s streams. PCBs were not detected. Time-weighted average water concentrations for the 2-month deployment periods were estimated from concentrations of chemicals measured in the passive samplers. Currently used pesticide concentrations peaked during spring and were detected beyond their seasons of expected use. Summed concentrations of legacy contaminants in Neal Creek were highest during July–Sept., the period with the lowest streamflows. Endosulfan was the only pesticide detected in passive samplers at concentrations exceeding Oregon or U.S. Environmental Protection Agency water-quality thresholds. A Sensitive Pesticide Toxicity Index (SPTI) was used to estimate the relative acute potential toxicity among sample mixtures. The acute potential toxicity of the detected mixtures was likely greater for invertebrates than for fish and for all samples in Neal Creek compared to Rogers Creek, but the indices appear to be low overall (<0.1). Endosulfans and pyrethroid insecticides were the largest contributors to the SPTIs for both sites. SPTIs of some discrete (grab) samples from the basin that were used for comparison exceeded 0.1 when some insecticides (azinphos methyl, chlorpyrifos, malathion) were detected at concentrations near or

  7. Year-Round Monitoring of Contaminants in Neal and Rogers Creeks, Hood River Basin, Oregon, 2011-12, and Assessment of Risks to Salmonids

    PubMed Central

    Hapke, Whitney B.; Morace, Jennifer L.; Nilsen, Elena B.; Alvarez, David A.; Masterson, Kevin

    2016-01-01

    Pesticide presence in streams is a potential threat to Endangered Species Act listed salmonids in the Hood River basin, Oregon, a primarily forested and agricultural basin. Two types of passive samplers, polar organic chemical integrative samplers (POCIS) and semipermeable membrane devices (SPMDs), were simultaneously deployed at four sites in the basin during Mar. 2011–Mar. 2012 to measure the presence of pesticides, polybrominated diphenyl ethers (PBDEs), and polychlorinated biphenyls (PCBs). The year-round use of passive samplers is a novel approach and offers several new insights. Currently used pesticides and legacy contaminants, including many chlorinated pesticides and PBDEs, were present throughout the year in the basin’s streams. PCBs were not detected. Time-weighted average water concentrations for the 2-month deployment periods were estimated from concentrations of chemicals measured in the passive samplers. Currently used pesticide concentrations peaked during spring and were detected beyond their seasons of expected use. Summed concentrations of legacy contaminants in Neal Creek were highest during July–Sept., the period with the lowest streamflows. Endosulfan was the only pesticide detected in passive samplers at concentrations exceeding Oregon or U.S. Environmental Protection Agency water-quality thresholds. A Sensitive Pesticide Toxicity Index (SPTI) was used to estimate the relative acute potential toxicity among sample mixtures. The acute potential toxicity of the detected mixtures was likely greater for invertebrates than for fish and for all samples in Neal Creek compared to Rogers Creek, but the indices appear to be low overall (<0.1). Endosulfans and pyrethroid insecticides were the largest contributors to the SPTIs for both sites. SPTIs of some discrete (grab) samples from the basin that were used for comparison exceeded 0.1 when some insecticides (azinphos methyl, chlorpyrifos, malathion) were detected at concentrations near or

  8. Long-term changes in river-floodplain dynamics: implications for salmonid habitat in the Interior Columbia Basin, USA.

    PubMed

    Tomlinson, Matthew J; Gergel, Sarah E; Beechie, Timothy J; McClure, Michelle M

    2011-07-01

    Rivers and their associated floodplains are among the world's most highly altered ecosystems, resulting in billions of dollars in restoration expenditures. Successful restoration of these systems requires information at multiple spatial scales (from localized reaches to broader-scale watersheds), as well as information spanning long time frames. Here, we develop a suite of historical landscape indicators of riverine status, primarily from the perspective of salmonid management, using a case study in the Interior Columbia Basin, Washington, USA. We use a combination of historical and modern aerial photography to quantify changes in land cover and reach type, as well as potential fish habitat within channel and off-channel floodplain areas. As of 1949, 55% of the Wenatchee River floodplain had been converted to agriculture. By 2006, 62% had been modified by anthropogenic development, of which 20% was due to urban expansion. The historical percentage of agricultural land in the watershed and the contemporary percentage of urban area surpass thresholds in land cover associated with deleterious impacts on river systems. In addition, the abundance of reach types associated with the highest quality salmonid habitat (island braided and meandering reaches) has declined due to conversion to straight reach types. The area occupied by fish habitats associated with channel migration (slow/stagnant channels and dry channels) has declined approximately 25-30%. Along highly modified rivers, these habitats have also become increasingly fragmented. Caveats related to visual quality and seasonal timing of historical photographs were important considerations in the interpretation of changes witnessed for headwater island braided systems, as well as for floodplain ponds. Development of rigorous, long-term, multi-scale monitoring techniques is necessary to guide the management and restoration of river-floodplain systems for the diversity of ecosystem services they provide.

  9. Trends in spawning populations of Pacific anadromous salmonids

    USGS Publications Warehouse

    Konkel, G.W.; McIntyre, J.D.

    1987-01-01

    Annual escapement records for 1968-1984 for five species of Pacific salmon-chinook (Oncorhynchus tshawytscha), coho (O. kisutch), sockeye (O. nerka), pink (O. gorbuscha), and chum (O. keta)—and steelhead (Salmo gairdneri) were obtained from published and unpublished sources and organized in a computer database. More than 25,500 escapement records were obtained for more than 1,100 locations throughout Alaska, Washington, Idaho, Oregon, and California. Escapement trends for naturally reproducing populations for which data were available for at least 7 years from 1968 to 1984 and at least 4 years from 1975 to 1984 were analyzed by linear regression. Significant trends were observed in about 30% of the 886 populations examined. Trends were summarized by species for three geographic regions in Alaska and four in the Pacific Northwest (including California). For chinook, sockeye, and pink salmon, trends were predominantly increasing in the Alaska regions and either lacking or predominantly decreasing in most of the Pacific Northwest regions; for coho and chum salmon, trends were predominantly decreasing in one or more Alaska regions as well as in most of the Pacific Northwest regions. For steelhead, too few populations were examined to enable us to characterize trends throughout their range. Among the 657 salmonid populations excluded from the trend analysis because the data sets were incomplete, 13 (of which 2 were in Alaska) declined to zero during the period of analysis. For coho, sockeye, pink, and chum salmon and steelhead, major data gaps were revealed by a comparison of the geographic distribution of escapement records with the spawning distribution of the species. For chinook salmon, escapement records were more geographically representative of the spawning distribution.

  10. Hydroacoustic Evaluation of Juvenile Salmonid Passage and Distribution at Lookout Point Dam, 2010

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Khan, Fenton; Johnson, Gary E.; Royer, Ida M.

    2011-07-01

    This report presents the results of an evaluation of juvenile salmonid passage and distribution at Lookout Point Dam (LOP) on the Middle Fork Willamette River. The study was conducted by the Pacific Northwest National Laboratory for the U.S. Army Corps of Engineers, Portland District (USACE). The goal of the study was to provide fish passage and distribution data to support decisions on long-term measures to enhance downstream passage at LOP and others dams in USACE’s Willamette Valley Project in response to the listing of Upper Willamette River Spring Chinook salmon (Oncorhynchus tshawytscha) and Upper Willamette River steelhead (O. mykiss) asmore » threatened under the Endangered Species Act. During the year-long study period - February 1, 2010 to January 31, 2011the objectives of the hydroacoustic evaluation of fish passage and distribution at LOP were to: 1. Estimate passage rates, run timing, horizontal distribution, and diel distribution at turbine penstock intakes for smolt-size fish. 2. Estimate passage rates, run timing and diel distribution at turbine penstock intakes for small-size fish. 3. Estimate passage rates and run timing at the regulating outlets for smolt-size fish. 4. Estimate vertical distribution of smolt-size fish in the forebay near the upstream face of the dam. The fixed-location hydroacoustic technique was used to accomplish the objectives of this study. Transducers (420 kHz) were deployed in each penstock intake, above each RO entrance, and on the dam face; a total of nine transducers (2 single-beam and 7 split-beam) were used. We summarize the findings from the hydroacoustic evaluation of juvenile salmonid passage and distribution at LOP during February 2010 through January 2011 as follows. • Fish passage rates for smolt-size fish (> ~90 mm) were highest during December-January and lowest in mid-summer through early fall. • During the entire study period, an estimated total of 142,463 fish ± 4,444 (95% confidence interval) smolt

  11. Denaturing gradient gel electrophoresis for nonlethal detection of Aeromonas salmonicida in salmonid mucus and its potential for other bacterial fish pathogens.

    PubMed

    Quinn, Robert A; Stevenson, Roselynn M W

    2012-05-01

    Denaturing gradient gel electrophoresis (DGGE) of 16S rDNA was used to nonlethally detect Aeromonas salmonicida and other bacteria in salmonid skin mucus. Mucus samples from wild spawning coho salmon (Oncorhynchus kisutch) with endemic A. salmonicida and from cultured lake trout (Salvelinus namaycush) were tested by PCR-DGGE and were compared with mucus culture on Coomassie brilliant blue agar and internal organ culture. PCR-DGGE gave a highly reproducible 4-band pattern for 9 strains of typical A. salmonicida, which was different from other Aeromonas spp. Aeromonas salmonicida presence in mucus was evident as a band that comigrated with the bottom band of the A. salmonicida 4-band pattern and was verified by sequencing. PCR-DGGE found 36 of 52 coho salmon positive for A. salmonicida, compared with 31 positive by mucus culture and 16 by organ culture. Numerous other bacteria were detected in salmonid mucus, including Pseudomonas spp., Shewanella putrefaciens, Aeromonas hydrophila and other aeromonads. However, Yersinia ruckeri was not detected in mucus from 27 lake trout, but 1 fish had a sorbitol-positive Y. ruckeri isolated from organ culture. Yersinia ruckeri seeded into a mucus sample suggested that PCR-DGGE detection of this bacterium from mucus was possible. PCR-DGGE allows nonlethal detection of A. salmonicida in mucus and differentiation of some Aeromonas spp. and has the potential to allow simultaneous detection of other pathogens present in fish mucus.

  12. The contribution of molecular epidemiology to the understanding and control of viral diseases of salmonid aquaculture

    PubMed Central

    2011-01-01

    Molecular epidemiology is a science which utilizes molecular biology to define the distribution of disease in a population (descriptive epidemiology) and relies heavily on integration of traditional (or analytical) epidemiological approaches to identify the etiological determinants of this distribution. The study of viral pathogens of aquaculture has provided many exciting opportunities to apply such tools. This review considers the extent to which molecular epidemiological studies have contributed to better understanding and control of disease in aquaculture, drawing on examples of viral diseases of salmonid fish of commercial significance including viral haemorrhagic septicaemia virus (VHSV), salmonid alphavirus (SAV) and infectious salmon anaemia virus (ISAV). Significant outcomes of molecular epidemiological studies include: Improved taxonomic classification of viruses A better understanding of the natural distribution of viruses An improved understanding of the origins of viral pathogens in aquaculture An improved understanding of the risks of translocation of pathogens outwith their natural host range An increased ability to trace the source of new disease outbreaks Development of a basis for ensuring development of appropriate diagnostic tools An ability to classify isolates and thus target future research aimed at better understanding biological function While molecular epidemiological studies have no doubt already made a significant contribution in these areas, the advent of new technologies such as pyrosequencing heralds a quantum leap in the ability to generate descriptive molecular sequence data. The ability of molecular epidemiology to fulfil its potential to translate complex disease pathways into relevant fish health policy is thus unlikely to be limited by the generation of descriptive molecular markers. More likely, full realisation of the potential to better explain viral transmission pathways will be dependent on the ability to assimilate and

  13. Migration depth and residence time of juvenile salmonids in the forebays of hydropower dams prior to passage through turbines or juvenile bypass systems: implications for turbine-passage survival.

    PubMed

    Li, Xinya; Deng, Zhiqun D; Brown, Richard S; Fu, Tao; Martinez, Jayson J; McMichael, Geoffrey A; Skalski, John R; Townsend, Richard L; Trumbo, Bradly A; Ahmann, Martin L; Renholds, Jon F

    2015-01-01

    Little is known about the three-dimensional depth distributions in rivers of individually marked fish that are in close proximity to hydropower facilities. Knowledge of the depth distributions of fish approaching dams can be used to understand how vulnerable fish are to injuries such as barotrauma as they pass through dams. To predict the possibility of barotrauma injury caused by pressure changes during turbine passage, it is necessary to understand fish behaviour relative to acclimation depth in dam forebays as they approach turbines. A guiding study was conducted using high-resolution three-dimensional tracking results of salmonids implanted with Juvenile Salmon Acoustic Telemetry System transmitters to investigate the depth distributions of subyearling and yearling Chinook salmon (Oncorhynchus tshawytscha) and juvenile steelhead (Oncorhynchus mykiss) passing two dams on the Snake River in Washington State. Multiple approaches were evaluated to describe the depth at which fish were acclimated, and statistical analyses were performed on large data sets extracted from ∼28 000 individually tagged fish during 2012 and 2013. Our study identified patterns of depth distributions of juvenile salmonids in forebays prior to passage through turbines or juvenile bypass systems. This research indicates that the median depth at which juvenile salmonids approached turbines ranged from 2.8 to 12.2 m, with the depths varying by species/life history, year, location (which dam) and diel period (between day and night). One of the most enlightening findings was the difference in dam passage associated with the diel period. The amount of time that turbine-passed fish spent in the immediate forebay prior to entering the powerhouse was much lower during the night than during the day. This research will allow scientists to understand turbine-passage survival better and enable them to assess more accurately the effects of dam passage on juvenile salmon survival.

  14. Migration depth and residence time of juvenile salmonids in the forebays of hydropower dams prior to passage through turbines or juvenile bypass systems: implications for turbine-passage survival

    PubMed Central

    Li, Xinya; Deng, Zhiqun D.; Brown, Richard S.; Fu, Tao; Martinez, Jayson J.; McMichael, Geoffrey A.; Skalski, John R.; Townsend, Richard L.; Trumbo, Bradly A.; Ahmann, Martin L.; Renholds, Jon F.

    2015-01-01

    Little is known about the three-dimensional depth distributions in rivers of individually marked fish that are in close proximity to hydropower facilities. Knowledge of the depth distributions of fish approaching dams can be used to understand how vulnerable fish are to injuries such as barotrauma as they pass through dams. To predict the possibility of barotrauma injury caused by pressure changes during turbine passage, it is necessary to understand fish behaviour relative to acclimation depth in dam forebays as they approach turbines. A guiding study was conducted using high-resolution three-dimensional tracking results of salmonids implanted with Juvenile Salmon Acoustic Telemetry System transmitters to investigate the depth distributions of subyearling and yearling Chinook salmon (Oncorhynchus tshawytscha) and juvenile steelhead (Oncorhynchus mykiss) passing two dams on the Snake River in Washington State. Multiple approaches were evaluated to describe the depth at which fish were acclimated, and statistical analyses were performed on large data sets extracted from ∼28 000 individually tagged fish during 2012 and 2013. Our study identified patterns of depth distributions of juvenile salmonids in forebays prior to passage through turbines or juvenile bypass systems. This research indicates that the median depth at which juvenile salmonids approached turbines ranged from 2.8 to 12.2 m, with the depths varying by species/life history, year, location (which dam) and diel period (between day and night). One of the most enlightening findings was the difference in dam passage associated with the diel period. The amount of time that turbine-passed fish spent in the immediate forebay prior to entering the powerhouse was much lower during the night than during the day. This research will allow scientists to understand turbine-passage survival better and enable them to assess more accurately the effects of dam passage on juvenile salmon survival. PMID:27293685

  15. Assessment of smolt condition: Biological and environmental interactions -- The impact of prey and predators on juvenile salmonids

    USGS Publications Warehouse

    Sauter, Sally T.; Schrock, Robin M.; Petersen, James H.; Maule, Alec G.

    2004-01-01

    The Bonneville Power Administration (BPA) has funded the Assessment of Smolt Condition project since 1987. During that time the project changed frequently to meet the information needs of fish managers by conducting studies throughout the Columbia River basin. Past research has examined the influence of smolt physiological development and health on migration rate; differences in development and migration rates of smolts of hatchery or wild origins; and the impacts of hatchery practices on smolt development. The Smolt Assessment Project will not continue beyond 2004, and here we report on the final study of the project in which we used bioenergetics modeling to investigate predation on juvenile salmonids by northern pikeminnow, smallmouth bass, and walleye in the lower Columbia River reservoirs.

  16. National Hydroelectric Power Resources Study: Regional Assessment: Volume XXII: Western Systems Coordinating Council, (WSCC). Volume 22

    DTIC Science & Technology

    1981-09-01

    respectively; the Klamath Mountains of Oregon and California; the Basin and Ranges of Nevada, the Teton Range of Wyoming; the Uinta Mountains of Utah...approximately 292,000 square miles, includes all of the Columbia River system in the United States and all other river basins in Idaho, Oregon, and...Central Valley and the Los Angeles Basin of California. The western valleys of the Pacific Northwest, the Denver-Cheyenne area along the Rockies’ eastern

  17. N-mix for fish: estimating riverine salmonid habitat selection via N-mixture models

    USGS Publications Warehouse

    Som, Nicholas A.; Perry, Russell W.; Jones, Edward C.; De Juilio, Kyle; Petros, Paul; Pinnix, William D.; Rupert, Derek L.

    2018-01-01

    Models that formulate mathematical linkages between fish use and habitat characteristics are applied for many purposes. For riverine fish, these linkages are often cast as resource selection functions with variables including depth and velocity of water and distance to nearest cover. Ecologists are now recognizing the role that detection plays in observing organisms, and failure to account for imperfect detection can lead to spurious inference. Herein, we present a flexible N-mixture model to associate habitat characteristics with the abundance of riverine salmonids that simultaneously estimates detection probability. Our formulation has the added benefits of accounting for demographics variation and can generate probabilistic statements regarding intensity of habitat use. In addition to the conceptual benefits, model application to data from the Trinity River, California, yields interesting results. Detection was estimated to vary among surveyors, but there was little spatial or temporal variation. Additionally, a weaker effect of water depth on resource selection is estimated than that reported by previous studies not accounting for detection probability. N-mixture models show great promise for applications to riverine resource selection.

  18. An epidemic model for the interactions between thermal regime of rivers and transmission of Proliferative Kidney Disease in salmonid fish

    NASA Astrophysics Data System (ADS)

    Carraro, Luca; Bertuzzo, Enrico; Mari, Lorenzo; Gatto, Marino; Strepparava, Nicole; Hartikainen, Hanna; Rinaldo, Andrea

    2015-04-01

    Proliferative kidney disease (PKD) affects salmonid populations in European and North-American rivers. It is caused by the endoparasitic myxozoan Tetracapsuloides bryosalmonae, which exploits freshwater bryozoans (Fredericella sultana) and salmonids as primary and secondary hosts, respectively. Incidence and mortality, which can reach up to 90-100%, are known to be strongly related to water temperature. PKD has been present in brown trout population for a long time but has recently increased rapidly in incidence and severity causing a decline in fish catches in many countries. In addition, environmental changes are feared to cause PKD outbreaks at higher latitude and altitude regions as warmer temperatures promote disease development. This calls for a better comprehension of the interactions between disease dynamics and the thermal regime of rivers, in order to possibly devise strategies for disease management. In this perspective, a spatially explicit model of PKD epidemiology in riverine host metacommunities is proposed. The model aims at summarizing the knowledge on the modes of transmission of the disease and the life-cycle of the parasite, making the connection between temperature and epidemiological parameters explicit. The model accounts for both local population and disease dynamics of bryozoans and fish and hydrodynamic dispersion of the parasite spores and hosts along the river network. The model is time-hybrid, coupling inter-seasonal and intra-seasonal dynamics, the former being described in a continuous time domain, the latter seen as time steps of a discrete time domain. In order to test the model, a case study is conducted in river Wigger (Cantons of Aargau and Lucerne, Switzerland), where data about water temperature, brown trout and bryozoan populations and PKD prevalence are being collected.

  19. Juvenile salmonid migratory behavior at the mouth of the Columbia River and within the plume

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McMichael, Geoffrey A.; O'Toole, Amanda C.; Harnish, Ryan A.

    A total of 8,159 acoustic-tagged salmonid smolts were detected at the mouth of the Columbia River. Of the fish detected at the mouth, 14% of yearling Chinook salmon, 9% of steelhead, and 22% of subyearling Chinook salmon were detected on a sparse array deployed in the Columbia River plume. Chinook salmon smolts decreased travel rate as they left the river and entered the plume, while steelhead increased travel rate. Chinook salmon also spent more time in the transitional area between the river mouth and plume as compared to steelhead. In early spring, yearling Chinook salmon and steelhead predominately migrated pastmore » the plume array towards the edge of the shelf and to the south. Later in the season, yearling Chinook salmon and steelhead smolts tended to migrate out of the river mouth in a northerly direction. Subyearling Chinook salmon migrated predominately past the portion of the plume array to the north of the river mouth.« less

  20. Removal of small dams and its influence on physical habitat for salmonids in a Norwegian river

    NASA Astrophysics Data System (ADS)

    Fjeldstad, Hans-Petter; Barlaup, Bjørn; Stickler, Morten; Alfredsen, Knut; Gabrielsen, Sven-Erik

    2010-05-01

    While research and implementation of upstream migration solutions is extensive, and indeed often successful, full scale restoration projects and investigations of their influence on fish biology are rare in Norway. Acid deposition in Norwegian catchments peaked in the 1980's and resulted in both chronically and episodically acidified rivers and Salmonids in River Nidelva, one of the largest cathments in southern Norway, where extinct for decades. During this period hydropower development in the river paid limited attention to aquatic ecology. Weirs were constructed for esthetic purposes in the late 1970's and turned a 3 km stretch into a lake habitat, well suited for lake dwelling fish species, but unsuited for migration, spawning and juvenile habitat for salmonids. Since 2005, continuous liming to mitigate acidification has improved the water quality and a program for reintroduction of Atlantic salmon has been implemented. We used hydraulic modeling to plan the removal of two weirs on a bypass reach of the river. The 50 meters wide concrete weirs were blasted and removed in 2007, and ecological monitoring has been carried out in the river to assess the effect of weir removal. Topographic mapping, hydraulic measurements and modeling, in combination with biological surveys before and after the removal of the weirs, has proved to represent a powerful method for design of physical habitat adjustments and assessing their influence on fish biology. The model results also supported a rapid progress of planning and executing of the works. While telemetry studies before weir removal suggested that adult migration past the weirs was delayed with several weeks the fish can now pass the reach with minor obstacles. Spawning sites were discovered in the old bed substrate and were occupied already the first season after water velocities increased to suitable levels for spawning. Accordingly, the densities of Atlantic salmon juveniles have shown a marked increased after the

  1. An online database for IHN virus in Pacific Salmonid fish: MEAP-IHNV

    USGS Publications Warehouse

    Kurath, Gael

    2012-01-01

    The MEAP-IHNV database provides access to detailed data for anyone interested in IHNV molecular epidemiology, such as fish health professionals, fish culture facility managers, and academic researchers. The flexible search capabilities enable the user to generate various output formats, including tables and maps, which should assist users in developing and testing hypotheses about how IHNV moves across landscapes and changes over time. The MEAP-IHNV database is available online at http://gis.nacse.org/ihnv/ (fig. 1). The database contains records that provide background information and genetic sequencing data for more than 1,000 individual field isolates of the fish virus Infectious hematopoietic necrosis virus (IHNV), and is updated approximately annually. It focuses on IHNV isolates collected throughout western North America from 1966 to the present. The database also includes a small number of IHNV isolates from Eastern Russia. By engaging the expertise of the broader community of colleagues interested in IHNV, our goal is to enhance the overall understanding of IHNV epidemiology, including defining sources of disease outbreaks and viral emergence events, identifying virus traffic patterns and potential reservoirs, and understanding how human management of salmonid fish culture affects disease. Ultimately, this knowledge can be used to develop new strategies to reduce the effect of IHN disease in cultured and wild fish.

  2. Screening of duplicated loci reveals hidden divergence patterns in a complex salmonid genome

    USGS Publications Warehouse

    Limborg, Morten T.; Larson, Wesley; Seeb, Lisa W.; Seeb, James E.

    2017-01-01

    A whole-genome duplication (WGD) doubles the entire genomic content of a species and is thought to have catalysed adaptive radiation in some polyploid-origin lineages. However, little is known about general consequences of a WGD because gene duplicates (i.e., paralogs) are commonly filtered in genomic studies; such filtering may remove substantial portions of the genome in data sets from polyploid-origin species. We demonstrate a new method that enables genome-wide scans for signatures of selection at both nonduplicated and duplicated loci by taking locus-specific copy number into account. We apply this method to RAD sequence data from different ecotypes of a polyploid-origin salmonid (Oncorhynchus nerka) and reveal signatures of divergent selection that would have been missed if duplicated loci were filtered. We also find conserved signatures of elevated divergence at pairs of homeologous chromosomes with residual tetrasomic inheritance, suggesting that joint evolution of some nondiverged gene duplicates may affect the adaptive potential of these genes. These findings illustrate that including duplicated loci in genomic analyses enables novel insights into the evolutionary consequences of WGDs and local segmental gene duplications.

  3. How Well Can We Predict Salmonid Spawning Habitat with LiDAR?

    NASA Astrophysics Data System (ADS)

    Pfeiffer, A.; Finnegan, N. J.; Hayes, S.

    2013-12-01

    Suitable salmonid spawning habitat is, to a great extent, determined by physical, landscape driven characteristics such as channel morphology and grain size. Identifying reaches with high-quality spawning habitat is essential to restoration efforts in areas where salmonid species are endangered or threatened. While both predictions of suitable habitat and observations of utilized habitat are common in the literature, they are rarely combined. Here we exploit a unique combination of high-resolution LiDAR data and seven years of 387 individually surveyed Coho and Steelhead redds in Scott Creek, a 77 km2 un-glaciated coastal California drainage in the Santa Cruz Mountains, to both make and test predictions of spawning habitat. Using a threshold channel assumption, we predict grain size throughout Scott Creek via a shear stress model that incorporates channel width, instead of height, using Manning's equation (Snyder et al., 2013). Slope and drainage area are computed from a LiDAR-derived DEM, and channel width is calculated via hydraulic modeling. Our results for median grain size predictions closely match median grain sizes (D50) measured in the field, with the majority of sites having predicted D50's within a factor of two of the observed values, especially for reaches with D50 > 0.02m. This success suggests that the threshold model used to predict grain size is appropriate for un-glaciated alluvial channel systems. However, it appears that grain size alone is not a strong predictor of salmon spawning. Reaches with a high (>0.1m) average predicted D50 do have lower redd densities, as expected based on spawning gravel sizes in the literature. However, reaches with lower (<0.1m) predicted D50 have a wide range of redd densities, suggesting that reach-average grain size alone cannot explain spawning site selection in the finer-grained reaches of Scott Creek. We turn to analysis of bedform morphology in order to explain the variation in redd density in the low

  4. A Quantitative Polymerase Chain Reaction Assay for the Detection and Quantification of Epizootic Epitheliotropic Disease Virus (EEDV; Salmonid Herpesvirus 3).

    PubMed

    Glenney, Gavin W; Barbash, Patricia A; Coll, John A

    2016-03-01

    Epizootic epitheliotropic disease virus (EEDV; salmonid herpesvirus [SalHV3]; family Alloherpesviridae) causes a systemic disease of juvenile and yearling Lake Trout Salvelinus namaycush. No cell lines are currently available for the culture and propagation of EEDV, so primary diagnosis is limited to PCR and electron microscopy. To better understand the pervasiveness of EEDV (carrier or latent state of infection) in domesticated and wild Lake Trout populations, we developed a sensitive TaqMan quantitative PCR (qPCR) assay to detect the presence of the EEDV terminase gene in Lake Trout tissues. This assay was able to detect a linear standard curve over nine logs of plasmid dilution and was sensitive enough to detect single-digit copies of EEDV. The efficiency of the PCR assay was 99.4 ± 0.06% (mean ± SD), with a 95% confidence limit of 0.0296 (R(2) = 0.994). Methods were successfully applied to collect preliminary data from a number of species and water bodies in the states of Pennsylvania, New York, and Vermont, indicating that EEDV is more common in wild fish than previously known. In addition, through the development of this qPCR assay, we detected EEDV in a new salmonid species, the Cisco Coregonus artedi. The qPCR assay was unexpectedly able to detect two additional herpesviruses, the Atlantic Salmon papillomatosis virus (ASPV; SalHV4) and the Namaycush herpesvirus (NamHV; SalHV5), which both share high sequence identity with the EEDV terminase gene. With these unexpected findings, we subsequently designed three primer sets to confirm initial TaqMan qPCR assay positives and to differentiate among EEDV, ASPV, and NamHV by detecting the glycoprotein genes via SYBR Green qPCR. Received April 20, 2015; accepted November 10, 2015.

  5. An epidemiological model of virus transmission in salmonid fishes of the Columbia River Basin

    USGS Publications Warehouse

    Ferguson, Paige F. B.; Breyta, Rachel; Brito, Ilana L.; Kurath, Gael; LaDeau, Shannon L.

    2018-01-01

    cohort-sites experienced self-exposure by infected juvenile fish, this transmission route had the greatest probability of infection (0.22). Increased testing and/or determining whether transmission can occur from cohort-sites without testing records (e.g., determining there was no testing record because there were no fish at the cohort-site) are expected to improve inference about infection probabilities. Increased use of secure water supplies and continued use of biosecurity protocols may reduce IHNV transmission from adult fish and juvenile fish within the site, respectively, to juvenile salmonids at hatcheries. Models and conclusions from this study are potentially relevant to understanding the relative importance of transmission routes for other important aquatic pathogens in salmonids, including the agents of bacterial kidney disease and coldwater disease, and the basic approach may be useful for other pathogens and hosts in other geographic regions.

  6. Risk assessment for the reintroduction of anadromous salmonids upstream of Chief Joseph and Grand Coulee Dams, Northeastern Washington

    USGS Publications Warehouse

    Hardiman, Jill M.; Breyta, Rachel B.; Haskell, Craig A.; Ostberg, Carl O.; Hatten, James R.; Connolly, Patrick J.

    2017-09-12

    The Upper Columbia United Tribes (UCUT; Spokane, Colville, Kootenai, Coeur d’Alene, and Kalispel Tribes) and Washington Department of Fish and Wildlife want to reintroduce anadromous salmonids to their historical range to restore ecosystem function and lost cultural and spiritual relationships in the upper Columbia River, northeastern Washington. The UCUT contracted with the U.S. Geological Survey to assess risks to resident taxa (existing fish populations in the reintroduction area upstream of Chief Joseph and Grand Coulee Dams) and reintroduced salmon associated with reintroduction. We developed a risk assessment framework for reintroduction of anadromous salmonids upstream of Chief Joseph and Grand Coulee Dams. To accomplish this goal, we applied strategies identified in previous risk assessment frameworks for reintroduction. The risk assessment is an initial step towards an anadromous reintroduction strategy. An initial list of potential donor sources for reintroduction species was developed from previous published sources for Chinook Salmon (Oncorhynchus tshawytscha) donors in the Transboundary Reach of the Columbia River, British Columbia; an ecological risk assessment of upper Columbia River hatchery programs on non-target taxa of concern; and a review of existing hatchery programsDuring two workshops, we further identified and ranked potential donor sources of anadromous Redband Trout (steelhead; O. mykiss), Chinook Salmon, Sockeye Salmon (O. nerka), and Coho Salmon (O. kisutch). We also identified resident fish populations of interest and their primary habitat, location, status, and pathogen concerns to determine the potential risks of reintroduction. Species were deemed of interest based on resource management and potential interactions (that is, genetics, competition, and predation) with introduced species. We developed tables of potential donors by species and characterized potential sources (hatchery and natural origins), populations (individual runs

  7. Survival and migration behavior of juvenile salmonids at McNary Dam, 2005: Final report of research

    USGS Publications Warehouse

    Perry, Russell W.; Braatz, Amy C.; Novick, Marc S.; Lucchesi, Joel N.; Rutz, Gary L.; Koch, Ryan C.; Schei, Jacquelyn L.; Adams, Noah S.; Rondorf, Dennis W.

    2007-01-01

    During 2005, we used radio-telemetry at McNary Dam to estimate passage and survival parameters of juvenile salmonids. During the spring migration period, two treatments were implemented: 1) 12-h spill operations with spill occurring between 1800 hours and 0600 hours, and 2) 24-h spill with spill occurring 24 hours per day. Treatments were not implemented during the summer migration period. However, a court-order was issued by Judge James Redden, U.S. 9th District Court, mandating a maximum powerhouse discharge of 50 kcfs with the remaining discharge to be spilled over the 24-h diel cycle between 1 July and 31 August 2005. Consequently, our study was conducted during two distinct periods: 1) a short period of involuntary spill for 24-h per day that occurred between 22 June and 30 June 2005, and 2) the period of court-ordered spill that was implemented after 1 July 2005.

  8. SALMOD: A population model for salmonids: user's manual. Version W3

    USGS Publications Warehouse

    Bartholow, John; Heasley, John; Laake, Jeff; Sandelin, Jeff; Coughlan, Beth A.K.; Moos, Alan

    2002-01-01

    SALMOD is a computer model that simulates the dynamics of freshwater salmonid populations, both anadromous and resident. The conceptual model was developed in a workshop setting (Williamson et al. 1993) using fish experts concerned with Trinity River chinook restoration. The model builds on the foundation laid by similar models (see Cheslak and Jacobson 1990). The model’s premise that that egg and fish mortality are directly related to spatially and temporally variable micro- and macrohabitat limitations, which themselves are related to the timing and amount of streamflow and other meteorological variables. Habitat quality and capacity are characterized by the hydraulic and thermal properties of individual mesohabitats, which we use as spatial “computation units” in the model. The model tracks a population of spatially distinct cohorts that originate as gees and grow from one life stage to another as a function of local water temperature. Individual cohorts either remain in the computational unit in which they emerged or move, in whole or in part, to nearby units (see McCormick et al. 1998). Model processes include spawning (with red superimposition and incubation losses), growth (including egg maturation), mortality, and movement (freshet-induced, habitat-induced, and seasonal). Model processes are implemented such that the user (modeler) has the ability to more-or-less program the model on the fly to create the dynamics thought to animate the population. SALMOD then tabulates the various causes of mortality and the whereabouts of fish.

  9. How sea lice from salmon farms may cause wild salmonid declines in Europe and North America and be a threat to fishes elsewhere

    PubMed Central

    Costello, Mark J.

    2009-01-01

    Fishes farmed in sea pens may become infested by parasites from wild fishes and in turn become point sources for parasites. Sea lice, copepods of the family Caligidae, are the best-studied example of this risk. Sea lice are the most significant parasitic pathogen in salmon farming in Europe and the Americas, are estimated to cost the world industry €300 million a year and may also be pathogenic to wild fishes under natural conditions. Epizootics, characteristically dominated by juvenile (copepodite and chalimus) stages, have repeatedly occurred on juvenile wild salmonids in areas where farms have sea lice infestations, but have not been recorded elsewhere. This paper synthesizes the literature, including modelling studies, to provide an understanding of how one species, the salmon louse, Lepeophtheirus salmonis, can infest wild salmonids from farm sources. Three-dimensional hydrographic models predicted the distribution of the planktonic salmon lice larvae best when they accounted for wind-driven surface currents and larval behaviour. Caligus species can also cause problems on farms and transfer from farms to wild fishes, and this genus is cosmopolitan. Sea lice thus threaten finfish farming worldwide, but with the possible exception of L. salmonis, their host relationships and transmission adaptations are unknown. The increasing evidence that lice from farms can be a significant cause of mortality on nearby wild fish populations provides an additional challenge to controlling lice on the farms and also raises conservation, economic and political issues about how to balance aquaculture and fisheries resource management. PMID:19586950

  10. Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids.

    PubMed

    Otterlei, Alexander; Brevik, Øyvind J; Jensen, Daniel; Duesund, Henrik; Sommerset, Ingunn; Frost, Petter; Mendoza, Julio; McKenzie, Peter; Nylund, Are; Apablaza, Patricia

    2016-03-15

    The study presents the phenotypic and genetic characterization of selected P. salmonis isolates from Atlantic salmon and rainbow trout suffering from SRS (salmonid rickettsial septicemia) in Chile and in Canada. The phenotypic characterization of the P. salmonis isolates were based on growth on different agar media (including a newly developed medium), different growth temperatures, antibiotics susceptibility and biochemical tests. This is the first study differentiating Chilean P. salmonis isolates into two separate genetic groups. Genotyping, based on 16S rRNA-ITS and concatenated housekeeping genes grouped the selected isolates into two clades, constituted by the Chilean strains, while the Canadian isolates form a branch in the phylogenetic tree. The latter consisted of two isolates that were different in both genetic and phenotypic characteristics. The phylogenies and the MLST do not reflect the origin of the isolates with respect to host species. The isolates included were heterogeneous in phenotypic tests. The genotyping methods developed in this study provided a tool for separation of P. salmonis isolates into distinct clades. The SRS outbreaks in Chile are caused by minimum two different genetic groups of P. salmonis. This heterogeneity should be considered in future development of vaccines against this bacterium in Chile. Two different strains of P. salmonis, in regards to genetic and phenotypic characteristics, can occur in the same contemporary outbreak of SRS.

  11. Needs Assessment Studies of Certified Nurse Assistants and Administrators. Volume XXII, No. 7.

    ERIC Educational Resources Information Center

    Fair, Lillian; And Others

    As part of an educational needs assessment, a survey was conducted by William Rainey Harper College (WRHC) in Palatine, Illinois, of Certified Nursing Assistants (CNA) and of administrators at institutions known to employ CNA's. Survey instruments were mailed to two groups. The first group, 153 administrators at institutions employing CNA's, were…

  12. Using bioenergetics modeling to estimate consumption of native juvenile salmonids by nonnative northern pike in the Upper Flathead River System, Montana

    USGS Publications Warehouse

    Muhlfeld, C.C.; Bennett, D.H.; Kirk, Steinhorst R.; Marotz, B.; Boyer, M.

    2008-01-01

    Introductions of nonnative northern pike Esox lucius have created recreational fisheries in many waters in the United States and Canada, yet many studies have shown that introduced northern pike may alter the composition and structure of fish communities through predation. We estimated the abundance of nonnative northern pike (2002-2003) and applied food habits data (1999-2003) to estimate their annual consumption of native bull trout Salvelinus confluentus and westslope cutthroat trout Oncorhynchus clarkii lewisi juveniles in the upper Flathead River system, Montana. Population estimates were generally consistent among years and ranged from 1,200 to 1,300 individuals. Westslope cutthroat trout were present in the diet of younger (???600 mm) and older (>600 mm) northern pike during all seasons and bull trout were found only in larger northern pike during all seasons but summer. Bioenergetics modeling estimated that the northern pike population annually consumed a total of 8.0 metric tons (mt) of fish flesh; the highest biomass was composed of cyprinids (4.95 mt) followed by whitefishes Prosopium spp. (1.02 mt), bull trout (0.80 mt), westslope cutthroat trout (0.68 mt), yellow perch Perca flavescens (0.41 mt),1 and other fishes (centrarchids and cottids; 0.14 mt). Numerically, the northern pike population consumed more than 342,000 fish; cyprinids and catostomids comprised approximately 82% of prey fish (278,925), whereas over 13,000 westslope cutthroat trout and nearly 3,500 bull trout were eaten, comprising about 5% of the prey consumed. Our results suggest that predation by introduced northern pike is contributing to the lower abundance of native salmonids in the system and that a possible benefit might accrue to native salmonids by reducing these predatory interactions. ?? Copyright by the American Fisheries Society 2008.

  13. Dextrans produced by lactic acid bacteria exhibit antiviral and immunomodulatory activity against salmonid viruses.

    PubMed

    Nácher-Vázquez, Montserrat; Ballesteros, Natalia; Canales, Ángeles; Rodríguez Saint-Jean, Sylvia; Pérez-Prieto, Sara Isabel; Prieto, Alicia; Aznar, Rosa; López, Paloma

    2015-06-25

    Viral infections in the aquaculture of salmonids can lead to high mortality and substantial economic losses. Thus, there is industrial interest in new molecules active against these viruses. Here we describe the production, purification, and the physicochemical and structural characterization of high molecular weight dextrans synthesized by Lactobacillus sakei MN1 and Leuconostoc mesenteroides RTF10. The purified dextrans, and commercial dextrans with molecular weights ranging from 10 to 2000kDa, were assayed in infected BF-2 and EPC fish cell-line monolayers for antiviral activity. Only T2000 and dextrans from MN1 and RTF10 had significant antiviral activity. This was similar to results obtained against infectious pancreatic necrosis virus. However the dextran from MN1 showed ten-fold higher activity against hematopoietic necrosis virus than T2000. In vivo assays using the MN1 polymer confirmed the in vitro results and revealed immunomodulatory activity. These results together with the high levels of dextran production (2gL(-1)) by Lb. sakei MN1, indicate the compounds potential utility as an antiviral agent in aquaculture. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. A multi-year analysis of spillway survival for juvenile salmonids as a function of spill bay operations at McNary Dam, Washington and Oregon, 2004-09

    USGS Publications Warehouse

    Adams, Noah S.; Hansel, Hal C.; Perry, Russell W.; Evans, Scott D.

    2012-01-01

    We analyzed 6 years (2004-09) of passage and survival data collected at McNary Dam to examine how spill bay operations affect survival of juvenile salmonids passing through the spillway at McNary Dam. We also examined the relations between spill bay operations and survival through the juvenile fish bypass in an attempt to determine if survival through the bypass is influenced by spill bay operations. We used a Cormack-Jolly-Seber release-recapture model (CJS model) to determine how the survival of juvenile salmonids passing through McNary Dam relates to spill bay operations. Results of these analyses, while not designed to yield predictive models, can be used to help develop dam-operation strategies that optimize juvenile salmonid survival. For example, increasing total discharge typically had a positive effect on both spillway and bypass survival for all species except sockeye salmon (Oncorhynchus nerka). Likewise, an increase in spill bay discharge improved spillway survival for yearling Chinook salmon (Oncorhynchus tshawytscha), and an increase in spillway discharge positively affected spillway survival for juvenile steelhead (Oncorhynchus mykiss). The strong linear relation between increased spill and increased survival indicates that increasing the amount of water through the spillway is one strategy that could be used to improve spillway survival for yearling Chinook salmon and juvenile steelhead. However, increased spill did not improve spillway survival for subyearling Chinook salmon and sockeye salmon. Our results indicate that a uniform spill pattern would provide the highest spillway survival and bypass survival for subyearling Chinook salmon. Conversely, a predominantly south spill pattern provided the highest spillway survival for yearling Chinook salmon and juvenile steelhead. Although spill pattern was not a factor for spillway survival of sockeye salmon, spill bay operations that optimize passage through the north and south spill bays maximized

  15. A fine-scale assessment of using barriers to conserve native stream salmonids: a case study in Akokala Creek, Glacier National Park, USA

    USGS Publications Warehouse

    Muhlfeld, Clint C.; D'Angelo, Vincent S.; S. T. Kalinowski,; Landguth, Erin L.; C. C. Downs,; J. Tohtz,; Kershner, Jeffrey L.

    2012-01-01

    Biologists are often faced with the difficult decision in managing native salmonids of where and when to install barriers as a conservation action to prevent upstream invasion of nonnative fishes. However, fine-scale approaches to assess long-term persistence of populations within streams and watersheds chosen for isolation management are often lacking. We employed a spatially-explicit approach to evaluate stream habitat conditions, relative abundance, and genetic diversity of native westslope cutthroat trout (Oncorhynchus clarkii lewisi) within the Akokala Creek watershed in Glacier National Park- a population threatened by introgressive hybridization with nonnative rainbow trout (O. mykiss) from nearby sources. The systematic survey of 24 stream reaches showed broad overlap in fish population and suitable habitat characteristics among reaches and no natural barriers to fish migration were found. Analysis of population structure using 16 microsatellite loci showed modest amounts of genetic diversity among reaches, and that fish from Long Bow Creek were the only moderately distinct genetic group. We then used this information to assess the potential impacts of three barrier placement scenarios on long-term population persistence and genetic diversity. The two barrier placement scenarios in headwater areas generally failed to meet general persistence criteria for minimum population size (2,500 individuals, Ne = 500), maintenance of long-term genetic diversity (He), and no population subdivision. Conversely, placing a barrier near the stream mouth and selectively passing non-hybridized, migratory spawners entering Akokala Creek met all persistence criteria and may offer the best option to conserve native trout populations and life history diversity. Systematic, fine-scale stream habitat, fish distribution, and genetic assessments in streams chosen for barrier installation are needed in conjunction with broader scale assessments to understand the potential impacts of

  16. Adaptive trade-offs in juvenile salmonid metabolism associated with habitat partitioning between coho salmon and steelhead trout in coastal streams.

    PubMed

    Van Leeuwen, Travis E; Rosenfeld, Jordan S; Richards, Jeffrey G

    2011-09-01

    1. Adaptive trade-offs are fundamental to the evolution of diversity and the coexistence of similar taxa and occur when complimentary combinations of traits maximize efficiency of resource exploitation or survival at different points on environmental gradients. 2. Standard metabolic rate (SMR) is a key physiological trait that reflects adaptations to baseline metabolic performance, whereas active metabolism reflects adaptations to variable metabolic output associated with performance related to foraging, predator avoidance, aggressive interactions or migratory movements. Benefits of high SMR and active metabolism may change along a resource (productivity) gradient, indicating that a trade-off exists among active metabolism, resting metabolism and energy intake. 3. We measured and compared SMR, maximal metabolic rate (MMR), aerobic scope (AS), swim performance (UCrit) and growth of juvenile hatchery and wild steelhead and coho salmon held on high- and low-food rations in order to better understand the potential significance of variation in SMR to growth, differentiation between species, and patterns of habitat use along a productivity gradient. 4. We found that differences in SMR, MMR, AS, swim performance and growth rate between steelhead trout and coho salmon were reduced in hatchery-reared fish compared with wild fish. Wild steelhead had a higher MMR, AS, swim performance and growth rate than wild coho, but adaptations between species do not appear to involve differences in SMR or to trade-off increased growth rate against lower swim performance, as commonly observed for high-growth strains. Instead, we hypothesize that wild steelhead may be trading off higher growth rate for lower food consumption efficiency, similar to strategies adopted by anadromous vs. resident brook trout and Atlantic salmon vs. brook trout. This highlights potential differences in food consumption and digestion strategies as cryptic adaptations ecologically differentiating salmonid species

  17. Development of a System-Wide Program, Volume II : Stepwise Implementation of a Predation Index, Predator Control Fisheries and Evaluation Plan in the Columbia River Basin, 1992 Annual Report.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ward, David L.; Nigro, Anthony A.; Willis, Charles F.

    1994-06-01

    The authors report their results of studies to determine the extent to which northern squawfish predation on juvenile salmonids is a problem in the Columbia River Basin, and to evaluate how effectively fisheries can be used to control northern squawfish populations and reduce juvenile salmonid losses to predation. These studies were initiated as part of a basinwide program to control northern squawfish predation and reduce mortality of juvenile salmonids on their migration to the ocean. Three papers are included in this report. They are entitled: (1) Development of a Systemwide Predator Control Program: Indexing and Fisheries Evaluation; (2) Economic, Socialmore » and Legal Feasibility of Commercial Sport, and Bounty Fisheries on Northern Squawfish; (3) Columbia River Ecosystem Model (CREM): Modeling Approach for Evaluation of Control of Northern Squawfish Populations using Fisheries Exploitation.« less

  18. Changes in Wisconsin's Lake Michigan salmonid sport fishery, 1969-1985

    USGS Publications Warehouse

    Hansen, Michael J.; Schultz, Paul T.; Lasee, Becky A.

    1990-01-01

    The modern sport fishery for salmonids in Wisconsin waters of Lake Michigan was begun during 1963-1969 with the stocking of rainbow trout (Oncorhynchus mykiss), lake trout (Salvelinus namaycush), brook trout (S. fontinalis), brown trout (Salmo trutta), coho salmon (O. kisutch), and chinook salmon (O. tshawytscha). The fishery grew rapidly during 1969-1985 as angler effort increased 10-fold, catch rate doubled, and catch increased 20-fold. The stocking and catch became increasingly dominated by chinook salmon, with coho salmon and lake trout of secondary importance and brown, rainbow, and brook trout of least importance. Trolling dominated the fishery, particularly by launched-boat anglers and, more recently, by moored-boat anglers. Charter boat trolling grew the most continuously and had the highest catch rates. The catch by trollers was dominated by chinook and coho salmon and lake trout. Pier, stream, and shore anglers fished less overall, but had catch rates that were similar to launched-boat anglers. The catch by pier and shore anglers was spread among chinook and coho salmon, and lake, brown and rainbow trout. The catch by stream anglers was dominated by chinook salmon. The percentage of stocked fish that were subsequently caught (catch ratio) was highest for fingerling chinook salmon (12.9%). Yearling brook trout, brown trout, coho salmon, lake trout, and rainbow trout had intermediate catch ratios (5.1-9.8%). Fingerling brook trout, brown trout, and lake trout had the lowest catch ratios (2.5-3.5%). The catch ratio for rainbow trout dropped from 9.8 to 5.1% after stocking with a different strain (the Shasta strain). Fingerling rainbow trout produced the lowest returns (<0.5%). We derived stocking recommendations for each species and life stage based on these catch ratios, and catch objectives based on maintaining catch levels recorded during 1983-1985.

  19. Efficiency of Portable Antennas for Detecting Passive Integrated Transponder Tags in Stream-Dwelling Salmonids

    PubMed Central

    Moyer, Katherine R.

    2016-01-01

    Portable antennas have become an increasingly common technique for tracking fish marked with passive integrated transponder (PIT) tags. We used logistic regression to evaluate how species, fish length, and physical habitat characteristics influence portable antenna detection efficiency in stream-dwelling brown trout (Salmo trutta), bull trout (Salvelinus confluentus), and redband trout (Oncorhynchus mykiss newberrii) marked with 12-mm PIT tags. We redetected 56% (20/36) of brown trout, 34% (68/202) of bull trout, and 33% (20/61) of redband trout after a recovery period of 21 to 46 hours. Models indicate support for length and species and minor support for percent boulder, large woody debris, and percent cobble as parameters important for describing variation in detection efficiency, although 95% confidence intervals for estimates were large. The odds of detecting brown trout (1.5 ± 2.2 [mean ± SE]) are approximately four times as high as bull trout (0.4 ± 1.6) or redband trout (0.3 ± 1.8) and species-specific differences may be related to length. Our reported detection efficiency for brown trout falls within the range of other studies, but is the first reported for bull trout and redband trout. Portable antennas may be a relatively unbiased way of redetecting varying sizes of all three salmonid species. PMID:26901317

  20. Efficiency of portable antennas for detecting passive integrated transponder tags in stream-dwelling salmonids

    USGS Publications Warehouse

    Banish, Nolan P.; Burdick, Summer M.; Moyer, Katherine R.

    2016-01-01

    Portable antennas have become an increasingly common technique for tracking fish marked with passive integrated transponder (PIT) tags. We used logistic regression to evaluate how species, fish length, and physical habitat characteristics influence portable antenna detection efficiency in stream-dwelling brown trout (Salmo trutta), bull trout (Salvelinus confluentus), and redband trout (Oncorhynchus mykiss newberrii) marked with 12-mm PIT tags. We redetected 56% (20/36) of brown trout, 34% (68/202) of bull trout, and 33% (20/61) of redband trout after a recovery period of 21 to 46 hours. Models indicate support for length and species and minor support for percent boulder, large woody debris, and percent cobble as parameters important for describing variation in detection efficiency, although 95% confidence intervals for estimates were large. The odds of detecting brown trout (1.5 ± 2.2 [mean ± SE]) are approximately four times as high as bull trout (0.4 ± 1.6) or redband trout (0.3 ± 1.8) and species-specific differences may be related to length. Our reported detection efficiency for brown trout falls within the range of other studies, but is the first reported for bull trout and redband trout. Portable antennas may be a relatively unbiased way of redetecting varying sizes of all three salmonid species.

  1. Fine-scale foraging movements by fish-eating killer whales (Orcinus orca) relate to the vertical distributions and escape responses of salmonid prey (Oncorhynchus spp.).

    PubMed

    Wright, Brianna M; Ford, John K B; Ellis, Graeme M; Deecke, Volker B; Shapiro, Ari Daniel; Battaile, Brian C; Trites, Andrew W

    2017-01-01

    We sought to quantitatively describe the fine-scale foraging behavior of northern resident killer whales ( Orcinus orca ), a population of fish-eating killer whales that feeds almost exclusively on Pacific salmon ( Oncorhynchus spp.). To reconstruct the underwater movements of these specialist predators, we deployed 34 biologging Dtags on 32 individuals and collected high-resolution, three-dimensional accelerometry and acoustic data. We used the resulting dive paths to compare killer whale foraging behavior to the distributions of different salmonid prey species. Understanding the foraging movements of these threatened predators is important from a conservation standpoint, since prey availability has been identified as a limiting factor in their population dynamics and recovery. Three-dimensional dive tracks indicated that foraging ( N  = 701) and non-foraging dives ( N  = 10,618) were kinematically distinct (Wilks' lambda: λ 16  = 0.321, P  < 0.001). While foraging, killer whales dove deeper, remained submerged longer, swam faster, increased their dive path tortuosity, and rolled their bodies to a greater extent than during other activities. Maximum foraging dive depths reflected the deeper vertical distribution of Chinook (compared to other salmonids) and the tendency of Pacific salmon to evade predators by diving steeply. Kinematic characteristics of prey pursuit by resident killer whales also revealed several other escape strategies employed by salmon attempting to avoid predation, including increased swimming speeds and evasive maneuvering. High-resolution dive tracks reconstructed using data collected by multi-sensor accelerometer tags found that movements by resident killer whales relate significantly to the vertical distributions and escape responses of their primary prey, Pacific salmon.

  2. A standard operating procedure for the surgical implantation of transmitters in juvenile salmonids

    USGS Publications Warehouse

    Liedtke, T.L.; Beeman, J.W.; Gee, L.P.

    2012-01-01

    Biotelemetry is a useful tool to monitor the movements of animals and is widely applied in fisheries research. Radio or acoustic technology can be used, depending on the study design and the environmental conditions in the study area. A broad definition of telemetry also includes the use of Passive Integrated Transponder (PIT) tags, either separately or with a radio or acoustic transmitter. To use telemetry, fish must be equipped with a transmitter. Although there are several attachment procedures available, surgical implantation of transmitters in the abdominal cavity is recognized as the best technique for long-term telemetry studies in general (Stasko and Pincock, 1977; Winter, 1996; Jepsen, 2003), and specifically for juvenile salmonids, Oncorhynchus spp. (Adams and others, 1998a, 1998b; Martinelli and others, 1998; Hall and others, 2009). Studies that use telemetry assume that the processes by which the animals are captured, handled, and tagged, as well as the act of carrying the transmitter, will have minimal effect on their behavior and performance. This assumption, commonly stated as a lack of transmitter effects, must be valid if telemetry studies are to describe accurately the movements and behavior of an entire population of interest, rather than the subset of that population that carries transmitters. This document describes a standard operating procedure (SOP) for surgical implantation of radio or acoustic transmitters in juvenile salmonids. The procedures were developed from a broad base of published information, laboratory experiments, and practical experience in tagging thousands of fish for numerous studies of juvenile salmon movements near Columbia River and Snake River hydroelectric dams. Staff from the Western Fisheries Research Center's Columbia River Research Laboratory (CRRL) frequently have used telemetry studies to evaluate new structures or operations at hydroelectric dams in the Columbia River Basin, and these evaluations typically

  3. Spectrum lines of highly ionized zinc, germanium, selenium, zirconium, molybdenum, and silver injected into Princeton Large Torus and Tokamak Fusion Test Reactor tokamak discharges

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hinnov, E.; Boody, F.; Cohen, S.

    1986-10-01

    Measured wavelengths of a number of highly ionized atoms are reported. These include the 3s/sup 2/3p--3s3p/sup 2/ and 3s/sup 2/3p--3s/sup 2/3d transitions in the aluminum isoelectronic sequence of Zn XVIII, Ge XX, Se XXII, Zr XXVIII, Mo XXX, and Ag XXXV; several transitions in the n = 2 shell of Zn XXII, Zn XXIII, and Zn XXIV; and the resonance and intercombination lines of Ag XXXVI--Ag XXXVII and of Ge XXIX--Ge XXX.

  4. Predicting the impacts of existing, pending, and future surface water rights on environmental flows to maintain anadromous salmonids in the northern California wine country

    NASA Astrophysics Data System (ADS)

    Deitch, M.; Kondolf, G. M.; Merenlender, A.; Cover, M. R.

    2006-12-01

    We used digitized aerial photographs on a geographical information system, historical stream flow records, and water rights records to model the effects of existing, pending, and future small reservoirs on stream flow on six tributaries to the Russian River in Sonoma County. Institutions governing whether these reservoirs can operate as constructed, and as proposed, has important implications for efforts to meet human and ecological water needs in the California wine country. Beginning in 1992, state agencies rewrote the policies governing how wine grape growers meet water needs to offer protections to endangered species and public trust values. These changes caused a shift in water management institutions: wine grape growers could no longer rely on surface water appropriations to meet growing water needs for new vineyards, and instead turned to other types of water rights that placed different (and potentially more severe) pressures on aquatic ecosystems. Despite growing controversy over the ecological impacts of existing and pending surface water appropriations (primarily small onstream and offstream reservoirs) on environmental flows necessary to support endangered anadromous salmonids, no analysis has been conducted to evaluate the impacts of existing small reservoirs, pending proposed reservoirs, or future reservoirs on local or catchment-scale stream flow. Our stream flow models indicated that existing and pending small reservoirs can eliminate flow immediately downstream of small reservoirs at the onset of the rainy season (when adult salmonids begin to migrate upstream to spawn); but the cumulative effect of several small reservoirs on stream reaches suitable for spawning is dampened by the spatial distribution of small reservoirs in a drainage network. The temporal extant of local flow effects is variable; most recent and pending onstream reservoirs can impair flows late into the rainy season, but their cumulative effects on downstream flows are less

  5. Comparison of pigment cell ultrastructure and organisation in the dermis of marble trout and brown trout, and first description of erythrophore ultrastructure in salmonids

    PubMed Central

    Djurdjevič, Ida; Kreft, Mateja Erdani; Sušnik Bajec, Simona

    2015-01-01

    Skin pigmentation in animals is an important trait with many functions. The present study focused on two closely related salmonid species, marble trout (Salmo marmoratus) and brown trout (S. trutta), which display an uncommon labyrinthine (marble-like) and spot skin pattern, respectively. To determine the role of chromatophore type in the different formation of skin pigment patterns in the two species, the distribution and ultrastructure of chromatophores was examined with light microscopy and transmission electron microscopy. The presence of three types of chromatophores in trout skin was confirmed: melanophores; xanthophores; and iridophores. In addition, using correlative microscopy, erythrophore ultrastructure in salmonids was described for the first time. Two types of erythrophores are distinguished, both located exclusively in the skin of brown trout: type 1 in black spot skin sections similar to xanthophores; and type 2 with a unique ultrastructure, located only in red spot skin sections. Morphologically, the difference between the light and dark pigmentation of trout skin depends primarily on the position and density of melanophores, in the dark region covering other chromatophores, and in the light region with the iridophores and xanthophores usually exposed. With larger amounts of melanophores, absence of xanthophores and presence of erythrophores type 1 and type L iridophores in the black spot compared with the light regions and the presence of erythrophores type 2 in the red spot, a higher level of pigment cell organisation in the skin of brown trout compared with that of marble trout was demonstrated. Even though the skin regions with chromatophores were well defined, not all the chromatophores were in direct contact, either homophilically or heterophilically, with each other. In addition to short-range interactions, an important role of the cellular environment and long-range interactions between chromatophores in promoting adult pigment pattern

  6. Comparison of pigment cell ultrastructure and organisation in the dermis of marble trout and brown trout, and first description of erythrophore ultrastructure in salmonids.

    PubMed

    Djurdjevič, Ida; Kreft, Mateja Erdani; Sušnik Bajec, Simona

    2015-11-01

    Skin pigmentation in animals is an important trait with many functions. The present study focused on two closely related salmonid species, marble trout (Salmo marmoratus) and brown trout (S. trutta), which display an uncommon labyrinthine (marble-like) and spot skin pattern, respectively. To determine the role of chromatophore type in the different formation of skin pigment patterns in the two species, the distribution and ultrastructure of chromatophores was examined with light microscopy and transmission electron microscopy. The presence of three types of chromatophores in trout skin was confirmed: melanophores; xanthophores; and iridophores. In addition, using correlative microscopy, erythrophore ultrastructure in salmonids was described for the first time. Two types of erythrophores are distinguished, both located exclusively in the skin of brown trout: type 1 in black spot skin sections similar to xanthophores; and type 2 with a unique ultrastructure, located only in red spot skin sections. Morphologically, the difference between the light and dark pigmentation of trout skin depends primarily on the position and density of melanophores, in the dark region covering other chromatophores, and in the light region with the iridophores and xanthophores usually exposed. With larger amounts of melanophores, absence of xanthophores and presence of erythrophores type 1 and type L iridophores in the black spot compared with the light regions and the presence of erythrophores type 2 in the red spot, a higher level of pigment cell organisation in the skin of brown trout compared with that of marble trout was demonstrated. Even though the skin regions with chromatophores were well defined, not all the chromatophores were in direct contact, either homophilically or heterophilically, with each other. In addition to short-range interactions, an important role of the cellular environment and long-range interactions between chromatophores in promoting adult pigment pattern

  7. Climate-induced trends in predator–prey synchrony differ across life-history stages of an anadromous salmonid

    USGS Publications Warehouse

    Bell, Donovan A.; Kovach, Ryan; Vulstek, Scott C.; Joyce, John E.; Tallmon, David A.

    2017-01-01

    Differential climate-induced shifts in phenology can create mismatches between predators and prey, but few studies have examined predator–prey mismatch across multiple life-history stages. We used long-term data from a warming stream with shifting salmonid migration timings to quantify intra-annual migration synchrony between predatory Dolly Varden (Salvelinus malma) and Pacific salmon prey and examined how predator–prey synchrony has been influenced by climate change. We demonstrate that Dolly Varden have become increasingly mismatched with spring downstream migrations of abundant pink salmon (Oncorhynchus gorbuscha) juveniles. However, Dolly Varden have remained matched with fall upstream migrations of spawning Pacific salmon, including coho (Oncorhynchus kisutch), sockeye (Oncorhynchus nerka), and pink salmon. Downstream predator–prey migration synchrony decreased over time and with higher temperatures, particularly with pink salmon. In contrast, upstream migration synchrony was temporally stable and increased with rising temperatures. Differing trends in Dolly Varden predator–prey synchrony may be explained by the direct use of salmon to cue upstream migration, but not downstream migration. Overall, we show that climate change can have differing impacts on predator–prey synchrony across life-history stages.

  8. Recovery of coded wire tags at a caspian tern colony in San Francisco Bay: A technique to evaluate impacts of avian predation on juvenile salmonids

    USGS Publications Warehouse

    Evans, A.F.; Roby, D.D.; Collis, K.; Cramer, B.M.; Sheggeby, J.A.; Adrean, L.J.; Battaglia, D.S.; Lyons, Donald E.

    2011-01-01

    We recovered coded wire tags (CWTs) from a colony of Caspian terns Hydroprogne caspia on Brooks Island in San Francisco Bay, California, to evaluate predation on juvenile salmonids originating from the Sacramento and San Joaquin rivers. Subsamples of colony substrate representing 11.7% of the nesting habitat used by the terns yielded 2,079 salmonid CWTs from fish released and subsequently consumed by terns in 2008. The estimated number of CWTs deposited on the entire tern colony was 40,143 (ranging from 26,763 to 80,288), once adjustments were made to account for tag loss and the total amount of nesting habitat used by terns. Tags ingested by terns and then egested on the colony were undamaged, and the tags' complete numeric codes were still identifiable. The CWTs found on the tern colony indicated that hatchery Chinook salmon Oncorhynchus tshawytscha trucked to and released in San Pablo Bay were significantly more likely to be consumed by Caspian terns than Chinook salmon that migrated in-river to the bay; 99.7% of all tags recovered were from bay-released Chinook salmon. Of the CWTs recovered on the tern colony, 98.0% were from fall-run Chinook salmon, indicating a higher susceptibility to tern predation than for the spring run type. None of the approximately 518,000 wild Chinook salmon that were coded-wire-tagged and released in the basin were recovered on the tern colony, suggesting that the impacts on wild, U.S. Endangered Species Act-listed Chinook salmon populations were minimal in 2008. Overall, we estimate that 0.3% of the approximately 12.3 million coded-wire-tagged Chinook salmon released in the basin in 2008 were subsequently consumed by Caspian terns from the Brooks Island colony. These results indicate that CWTs implanted in juvenile salmon can be recovered from a piscivorous waterbird colony and used to evaluate smolt losses for runs that are tagged. Abstract We recovered coded wire tags (CWTs) from a colony of Caspian terns Hydroprogne caspia on

  9. Characterizing the distribution of an endangered salmonid using environmental DNA analysis

    USGS Publications Warehouse

    Laramie, Matthew B.; Pilliod, David S.; Goldberg, Caren S.

    2015-01-01

    Determining species distributions accurately is crucial to developing conservation and management strategies for imperiled species, but a challenging task for small populations. We evaluated the efficacy of environmental DNA (eDNA) analysis for improving detection and thus potentially refining the known distribution of Chinook salmon (Oncorhynchus tshawytscha) in the Methow and Okanogan Subbasins of the Upper Columbia River, which span the border between Washington, USA and British Columbia, Canada. We developed an assay to target a 90 base pair sequence of Chinook DNA and used quantitative polymerase chain reaction (qPCR) to quantify the amount of Chinook eDNA in triplicate 1-L water samples collected at 48 stream locations in June and again in August 2012. The overall probability of detecting Chinook with our eDNA method in areas within the known distribution was 0.77 (±0.05 SE). Detection probability was lower in June (0.62, ±0.08 SE) during high flows and at the beginning of spring Chinook migration than during base flows in August (0.93, ±0.04 SE). In the Methow subbasin, mean eDNA concentration was higher in August compared to June, especially in smaller tributaries, probably resulting from the arrival of spring Chinook adults, reduced discharge, or both. Chinook eDNA concentrations did not appear to change in the Okanogan subbasin from June to August. Contrary to our expectations about downstream eDNA accumulation, Chinook eDNA did not decrease in concentration in upstream reaches (0–120 km). Further examination of factors influencing spatial distribution of eDNA in lotic systems may allow for greater inference of local population densities along stream networks or watersheds. These results demonstrate the potential effectiveness of eDNA detection methods for determining landscape-level distribution of anadromous salmonids in large river systems.

  10. Sensitivity of salmonid freshwater life history in western US streams to future climate conditions.

    PubMed

    Beer, W Nicholas; Anderson, James J

    2013-08-01

    We projected effects of mid-21st century climate on the early life growth of Chinook salmon (Oncorhynchus tshawytscha) and steelhead (O. mykiss) in western United States streams. Air temperature and snowpack trends projected from observed 20th century trends were used to predict future seasonal stream temperatures. Fish growth from winter to summer was projected with temperature-dependent models of egg development and juvenile growth. Based on temperature data from 115 sites, by mid-21st century, the effects of climate change are projected to be mixed. Fish in warm-region streams that are currently cooled by snow melt will grow less, and fish in suboptimally cool streams will grow more. Relative to 20th century conditions, by mid-21st century juvenile salmonids' weights are expected to be lower in the Columbia Basin and California Central Valley, but unchanged or greater in coastal and mountain streams. Because fish weight affects fish survival, the predicted changes in weight could impact population fitness depending on other factors such as density effects, food quality and quantity changes, habitat alterations, etc. The level of year-to-year variability in stream temperatures is high and our analysis suggests that identifying effects of climate change over the natural variability will be difficult except in a few streams. © 2013 John Wiley & Sons Ltd.

  11. Neural network detected in a presumed vestigial trait: ultrastructure of the salmonid adipose fin

    PubMed Central

    Buckland-Nicks, J. A.; Gillis, M.; Reimchen, T. E.

    2012-01-01

    A wide variety of rudimentary and apparently non-functional traits have persisted over extended evolutionary time. Recent evidence has shown that some of these traits may be maintained as a result of developmental constraints or neutral energetic cost, but for others their true function was not recognized. The adipose fin is small, fleshy, non-rayed and located between the dorsal and caudal fins on eight orders of basal teleosts and has traditionally been regarded as vestigial without clear function. We describe here the ultrastructure of the adipose fin and for the first time, to our knowledge, present evidence of extensive nervous tissue, as well as an unusual subdermal complex of interconnected astrocyte-like cells equipped with primary cilia. The fin contains neither adipose tissue nor fin rays. Many fusiform actinotrichia, comprising dense striated macrofibrils, support the free edge and connect with collagen cables that link the two sides. These results are consistent with a recent hypothesis that the adipose fin may act as a precaudal flow sensor, where its removal can be detrimental to swimming efficiency in turbulent water. Our findings provide insight to the broader themes of function versus constraints in evolutionary biology and may have significance for fisheries science, as the adipose fin is routinely removed from millions of salmonids each year. PMID:21733904

  12. Evolutionary effects of alternative artificial propagation programs: implications for viability of endangered anadromous salmonids

    PubMed Central

    McClure, Michelle M; Utter, Fred M; Baldwin, Casey; Carmichael, Richard W; Hassemer, Peter F; Howell, Philip J; Spruell, Paul; Cooney, Thomas D; Schaller, Howard A; Petrosky, Charles E

    2008-01-01

    Most hatchery programs for anadromous salmonids have been initiated to increase the numbers of fish for harvest, to mitigate for habitat losses, or to increase abundance in populations at low abundance. However, the manner in which these programs are implemented can have significant impacts on the evolutionary trajectory and long-term viability of populations. In this paper, we review the potential benefits and risks of hatchery programs relative to the conservation of species listed under the US Endangered Species Act. To illustrate, we present the range of potential effects within a population as well as among populations of Chinook salmon (Oncorhynchus tshawytscha) where changes to major hatchery programs are being considered. We apply evolutionary considerations emerging from these examples to suggest broader principles for hatchery uses that are consistent with conservation goals. We conclude that because of the evolutionary risks posed by artificial propagation programs, they should not be viewed as a substitute for addressing other limiting factors that prevent achieving viability. At the population level, artificial propagation programs that are implemented as a short-term approach to avoid imminent extinction are more likely to achieve long-term population viability than approaches that rely on long-term supplementation. In addition, artificial propagation programs can have out-of-population impacts that should be considered in conservation planning. PMID:25567637

  13. Hydroacoustic Evaluation of Juvenile Salmonid Passage and Distribution at Lookout Point Dam, 2010

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Khan, Fenton; Johnson, Gary E.; Royer, Ida M.

    Pacific Northwest National Laboratory evaluated juvenile salmonid passage and distribution at Lookout Point Dam (LOP) on the Middle Fork Willamette River for the U.S. Army Corps of Engineers, Portland District (USACE), to provide data to support decisions on long-term measures to enhance downstream passage at LOP and others dams in USACE's Willamette Valley Project. This study was conducted in response to the listing of Upper Willamette River Spring Chinook salmon (Oncorhynchus tshawytscha) and Upper Willamette River steelhead (O. mykiss) as threatened under the Endangered Species Act. We conducted a hydroacoustic evaluation of juvenile salmonid passage and distribution at LOP duringmore » February 2010 through January 2011. Findings from this 1 year of study should be applied carefully because annual variation can be expected due to variability in adult salmon escapement, egg-to-fry and fry-to-smolt survival rates, reservoir rearing and predation, dam operations, and weather. Fish passage rates for smolt-size fish (> {approx}90 mm and < 300 mm) were highest during December-January and lowest in mid-summer through early fall. Passage peaks were also evident in early spring, early summer, and late fall. During the entire study period, an estimated total of 142,463 fish {+-} 4,444 (95% confidence interval) smolt-size fish passed through turbine penstock intakes. Of this total, 84% passed during December-January. Run timing for small-size fish ({approx}65-90 mm) peaked (702 fish) on December 18. Diel periodicity of smolt-size fish showing crepuscular peaks was evident in fish passage into turbine penstock intakes. Relatively few fish passed into the Regulating Outlets (ROs) when they were open in summer (2 fish/d) and winter (8 fish/d). Overall, when the ROs were open, RO efficiency (RO passage divided by total project passage) was 0.004. In linear regression analyses, daily fish passage (turbines and ROs combined) for smolt-size fish was significantly related to

  14. Understanding Multiscale Surface Water-Groundwater Interactions on Scott River Watershed Temperatures with the use of Distributed Temperature Sensing (DTS) in Support of the Coldwater Salmonid Fishery Beneficial Use

    NASA Astrophysics Data System (ADS)

    Hines, R. J.; Harter, T.; Tyler, S. W.; McFadin, B.; Yokel, E.

    2008-12-01

    The Scott River is a major tributary to the Klamath River that provides cold water rearing habitat for wild salmonid populations, including coho salmon (Oncorhynchus kisutch), Chinook salmon (O. tshawytscha), and steelhead trout (O. mykiss). During the summer months (July through September), the main-stem Scott River becomes disconnected from its tributaries throughout much of Scott Valley and relies primarily on baseflow from the groundwater aquifer. Summer stream temperatures in the Scott River are currently at levels that are not considered sustainable for the native salmonid population, resulting in the enactment of a Total Maximum Daily Load (TMDL) for temperature. Two of the conditions affecting stream temperature have been identified as increases in solar radiation due to a reduction in riparian vegetation and decreased accretion of groundwater. In conjunction with a regional scale surface water-groundwater modeling effort to investigate the benefits of various conjunctive use management alternatives on mid- and late summer baseflow in the Scott River, we completed high-resolution field measurements of stream temperature over an approximately 1,050-meter reach. Temperatures were measured using Fiber-Optic Distributed Temperature Sensing (DTS) techniques. The DTS survey in combination with FLIR stream surface temperature data from 2003 indicate that groundwater discharge to the Scott River is highly localized throughout the valley. The results of the DTS survey depict highly localized areas of groundwater accretion, as well as prominent localized temperature effects from riparian vegetation and river geomorphology. While originally modeled as a well-mixed stream during FLIR analysis, the DTS data further suggest that locally strong, vertical thermal gradients are found near the bottom of the active stream channel. The high-resolution temperature measurements were paired with fish surveys in order to determine the correlation between areas of identified lower

  15. Hydroacoustic Evaluation of Juvenile Salmonid Passage and Distribution at Detroit Dam, 2011

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Khan, Fenton; Royer, Ida M.; Johnson, Gary E.

    Pacific Northwest National Laboratory evaluated juvenile salmonid passage and distribution at Detroit Dam (DET) on the North Santiam River, Oregon for the U.S. Army Corps of Engineers (USACE) to provide data to support decisions on long-term measures to enhance downstream passage at DET and others dams in USACE’s Willamette Valley Project. This study was conducted in response to regulatory requirements necessitated by the listing of Upper Willamette River Spring Chinook salmon (Oncorhynchus tshawytscha) and Upper Willamette River steelhead (O. mykiss) as threatened under the Endangered Species Act. The goal of the study was to provide information of juvenile salmonid passagemore » and distribution at DET from February 2011 through February 2012. The results of the hydroacoustic study provide new and, in some cases, first-ever data on passage estimates, run timing, distributions, and relationships between fish passage and environmental variables at the dam. This information will inform management decisions on the design and development of surface passage and collection devices to help restore Chinook salmon populations in the North Santiam River watershed above DET. During the entire study period, an estimated total of 182,526 smolt-size fish (±4,660 fish, 95% CI) passed through turbine penstock intakes. Run timing peaked in winter and early spring months. Passage rates were highest during late fall, winter and early spring months and low during summer. Horizontal distribution for hours when both turbine units were operated simultaneously indicated Unit 2 passed almost twice as much fish as Unit 1. Diel distribution for smolt-size fish during the study period was fairly uniform, indicating fish were passing the turbines at all times of the day. A total of 5,083 smolt-size fish (± 312 fish, 95% CI) were estimated passed via the spillway when it was open between June 23 and September 27, 2011. Daily passage was low at the spillway during the June-August period

  16. Genetic and serological diversity of Flavobacterium psychrophilum isolates from salmonids in United Kingdom.

    PubMed

    Ngo, Thao P H; Bartie, Kerry L; Thompson, Kim D; Verner-Jeffreys, David W; Hoare, Rowena; Adams, Alexandra

    2017-03-01

    Flavobacterium psychrophilum is one of the most important bacterial pathogens affecting cultured rainbow trout (Oncorhynchus mykiss) and is increasingly causing problems in Atlantic salmon (Salmo salar L.) hatcheries. Little is known about the heterogeneity of F. psychrophilum isolates on UK salmonid farms. A total of 315 F. psychrophilum isolates, 293 of which were collected from 27 sites within the UK, were characterised using four genotyping methods and a serotyping scheme. A high strain diversity was identified among the isolates with 54 pulsotypes, ten (GTG) 5 -PCR types, two 16S rRNA allele lineages, seven plasmid profiles and three serotypes. Seven PFGE groups and 27 singletons were formed at a band similarity of 80%. PFGE group P (n=75) was found to be numerically predominant in eight sites within the UK. Two major PFGE clusters and 13 outliers were found at the band similarity of 40%. The predominant profileobserved within the F. psychrophilum isolates examined was PFGE cluster II - (GTG) 5 -PCR type r1-16S rRNA lineage II - serotype Th (70/156 isolates examined, 45%). Co-existence of genetically and serologically heterogeneous isolates within each farm was detected, confounding the ability to control RTFS outbreaks. The occurrence over time (up to 11 years) of F. psychrophilum pulsotypes in three representative sites (Scot I, Scot III and Scot V) within Scotland was examined, potentially providing important epidemiological data for farm management and the development of site-specific vaccines. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Spiral swimming behavior due to cranial and vertebral lesions associated with Cytophaga psychrophila infections in salmonid fishes

    USGS Publications Warehouse

    Kent, M.L.; Groff, J.M.; Morrison, J.K.; Yasutake, W.T.; Holt, R.A.

    1989-01-01

    C. psychrophila infections of the cranium and anterior vertebrae in salmonid fishes were associated with ataxia, spiral swimming along the axis of the fish, and death. The syndrome was observed in 2-10% of underyearling coho salmon Oncorhynchus kisutch, rainbow troutSalmo gairdneri, and steelhead trout S. gairdneri at several private, state, and federal hatcheries in Washington and Oregon, USA, between 1963 and 1987. Affected fish did not recover and ultimately died. Histological examination consistently revealed subacute to chronic periostitis, osteitis, meningitis, and ganglioneuritis. Inflammation and periosteal proliferation of the anterior vertebrae at the junction of the vertebral column with the cranium with extension into the cranial case was a consistent feature. The adjacent nervous tissue, particularly the medulla, was often compressed by the proliferative lesion, and this may have caused the ataxia. Though bacteria were seldom observed in these lesions. C. psychrophilawas isolated in culture from the cranial cavity of all affected fish that were tested. Epidemiological observations suggested that this bacterium is the causative agent because the spiral swimming behaviour and lesions were observed only in populations that had recovered from acute C. psychrophila infections.

  18. 'Candidatus Phytoplasma palmicola', associated with a lethal yellowing-type disease of coconut (Cocos nucifera L.) in Mozambique.

    PubMed

    Harrison, Nigel A; Davis, Robert E; Oropeza, Carlos; Helmick, Ericka E; Narváez, María; Eden-Green, Simon; Dollet, Michel; Dickinson, Matthew

    2014-06-01

    In this study, the taxonomic position and group classification of the phytoplasma associated with a lethal yellowing-type disease (LYD) of coconut (Cocos nucifera L.) in Mozambique were addressed. Pairwise similarity values based on alignment of nearly full-length 16S rRNA gene sequences (1530 bp) revealed that the Mozambique coconut phytoplasma (LYDM) shared 100% identity with a comparable sequence derived from a phytoplasma strain (LDN) responsible for Awka wilt disease of coconut in Nigeria, and shared 99.0-99.6% identity with 16S rRNA gene sequences from strains associated with Cape St Paul wilt (CSPW) disease of coconut in Ghana and Côte d'Ivoire. Similarity scores further determined that the 16S rRNA gene of the LYDM phytoplasma shared <97.5% sequence identity with all previously described members of 'Candidatus Phytoplasma'. The presence of unique regions in the 16S rRNA gene sequence distinguished the LYDM phytoplasma from all currently described members of 'Candidatus Phytoplasma', justifying its recognition as the reference strain of a novel taxon, 'Candidatus Phytoplasma palmicola'. Virtual RFLP profiles of the F2n/R2 portion (1251 bp) of the 16S rRNA gene and pattern similarity coefficients delineated coconut LYDM phytoplasma strains from Mozambique as novel members of established group 16SrXXII, subgroup A (16SrXXII-A). Similarity coefficients of 0.97 were obtained for comparisons between subgroup 16SrXXII-A strains and CSPW phytoplasmas from Ghana and Côte d'Ivoire. On this basis, the CSPW phytoplasma strains were designated members of a novel subgroup, 16SrXXII-B.

  19. Global sensitivity analysis of water age and temperature for informing salmonid disease management

    NASA Astrophysics Data System (ADS)

    Javaheri, Amir; Babbar-Sebens, Meghna; Alexander, Julie; Bartholomew, Jerri; Hallett, Sascha

    2018-06-01

    Many rivers in the Pacific Northwest region of North America are anthropogenically manipulated via dam operations, leading to system-wide impacts on hydrodynamic conditions and aquatic communities. Understanding how dam operations alter abiotic and biotic variables is important for designing management actions. For example, in the Klamath River, dam outflows could be manipulated to alter water age and temperature to reduce risk of parasite infections in salmon by diluting or altering viability of parasite spores. However, sensitivity of water age and temperature to the riverine conditions such as bathymetry can affect outcomes from dam operations. To examine this issue in detail, we conducted a global sensitivity analysis of water age and temperature to a comprehensive set of hydraulics and meteorological parameters in the Klamath River, California, where management of salmonid disease is a high priority. We applied an analysis technique, which combined Latin-hypercube and one-at-a-time sampling methods, and included simulation runs with the hydrodynamic numerical model of the Lower Klamath. We found that flow rate and bottom roughness were the two most important parameters that influence water age. Water temperature was more sensitive to inflow temperature, air temperature, solar radiation, wind speed, flow rate, and wet bulb temperature respectively. Our results are relevant for managers because they provide a framework for predicting how water within 'high infection risk' sections of the river will respond to dam water (low infection risk) input. Moreover, these data will be useful for prioritizing the use of water age (dilution) versus temperature (spore viability) under certain contexts when considering flow manipulation as a method to reduce risk of infection and disease in Klamath River salmon.

  20. Sources of variability and comparability between salmonid stomach contents and isotopic analyses: study design lessons and recommendations

    USGS Publications Warehouse

    Vinson, M.R.; Budy, P.

    2011-01-01

    We compared sources of variability and cost in paired stomach content and stable isotope samples from three salmonid species collected in September 2001–2005 and describe the relative information provided by each method in terms of measuring diet overlap and food web study design. Based on diet analyses, diet overlap among brown trout, rainbow trout, and mountain whitefish was high, and we observed little variation in diets among years. In contrast, for sample sizes n ≥ 25, 95% confidence interval (CI) around mean δ15Ν and δ13C for the three target species did not overlap, and species, year, and fish size effects were significantly different, implying that these species likely consumed similar prey but in different proportions. Stable isotope processing costs were US$12 per sample, while stomach content analysis costs averaged US$25.49 ± $2.91 (95% CI) and ranged from US$1.50 for an empty stomach to US$291.50 for a sample with 2330 items. Precision in both δ15Ν and δ13C and mean diet overlap values based on stomach contents increased considerably up to a sample size of n = 10 and plateaued around n = 25, with little further increase in precision.

  1. Determination of an organic-acid analog of DOC for use in copper toxicity studies on salmonids

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    MacRae, R.K.; Meyer, J.S.; Hansen, J.A.

    1995-12-31

    Concentrations of dissolved copper in streams draining mine sites often exceed concentrations shown to cause acute and chronic mortality in salmonids. However, toxicity and impaired behaviors may be modified by dissolved organic carbon (DOC) and other inorganic components present in the site water. The effects of DOC on copper speciation, and thus bioavailability and toxicity, were determined by titrating stream waters with copper, using a cupric ion-specific electrode to detect free copper concentrations. Effects of various competing cations (e.g., Ca{sup +2}, Co{sup +2}) on copper-DOC binding were also evaluated. Titration results were evaluated using Scatchard and non-linear regression analyses tomore » quantify the strength and capacity of copper-DOC binding. Inorganic speciation was determined using the geochemical model MINEQL{sup +}. Results of these titrations indicated the presence of two or three distinct copper binding components in site water DOC. Three commercially available organic acids where then chosen to mimic the binding characteristics of natural DOC. This DOC-analog was used successfully in fish toxicity studies to evaluate the influence of DOC on copper bioavailability. Geochemical models were developed to predict copper speciation in both laboratory test waters and site waters, for any typical combination of water chemistry parameters (pH, alkalinity, [DOC], etc.). A combined interpretation of fish toxicity and modeling results indicate that some DOC-bound copper was bioavailable.« less

  2. Survival and migration behavior of juvenile salmonids at McNary Dam, 2004, Final report of research

    USGS Publications Warehouse

    Perry, Russell W.; Braatz, Amy C.; Fielding, Scott D.; Lucchesi, Joel N.; Plumb, John M.; Adams, Noah S.; Rondorf, Dennis W.

    2005-01-01

    During 2004, the USGS Columbia River Research Laboratory conducted a study at McNary Dam using radio telemetry to estimate passage and survival parameters of juvenile salmonids. Our primary objective was to estimate these parameters under ambient environmental and operational conditions, and thus project-wide treatments were not implemented. The primary dam operation consisted of “biop” spill, where spill occurred at night between 1800 and 0600 hours, and no spill occurred between 0600 and 1800 hours for the majority of our study period. During the spring study period, we radio-tagged and released 1,896 yearling Chinook salmon and 1,888 juvenile steelhead. During the summer study period, we radio-tagged and released 1,919 subyearling Chinook salmon. All fish were tagged using gastric techniques to implant transmitters weighing 1.58 g for yearling Chinook salmon, 1.93 g for juvenile steelhead, and 0.96 g for subyearling Chinook salmon. Minimum fish sizes were based on a 6.5% tag:fish weight ratio, and the size of tagged fish represented about 91%, 100%, and 17% of the population, respectively for yearling Chinook salmon, juvenile steelhead, and subyearling Chinook salmon. About 60% of radio-tagged fish were released 10 km upstream of McNary Dam at Hat Rock State Park, Oregon, with the remainder released as control groups 400 m downstream of the dam.

  3. Acoustic Imaging Evaluation of Juvenile Salmonid Behavior in the Immediate Forebay of the Water Temperature Control Tower at Cougar Dam, 2010

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Khan, Fenton; Johnson, Gary E.; Royer, Ida M.

    This report presents the results of an evaluation of juvenile Chinook salmon (Oncorhynchus tshawytscha) behavior at Cougar Dam on the south fork of the McKenzie River in Oregon in 2010. The study was conducted by the Pacific Northwest National Laboratory for the U.S. Army Corps of Engineers (USACE). The overall goal of the study was to characterize juvenile salmonid behavior and movement patterns in the immediate forebay of the Water Temperature Control (WTC) tower of the dam for USACE and fisheries resource managers use in making decisions about bioengineering designs for long-term structures and/or operations to facilitate safe downstream passagemore » for juvenile salmonids. We collected acoustic imaging (Dual-Frequency Identification Sonar; DIDSON) data from March 1, 2010, through January 31, 2011. Juvenile salmonids (hereafter, called 'fish') were present in the immediate forebay of the WTC tower throughout the study. Fish abundance index was low in early spring (<200 fish per sample-day), increased in late April, and peaked on May 19 (6,039 fish). A second peak was observed on June 6 (2904 fish). Fish abundance index decreased in early June and remained low in the summer months (<100 fish per sample-day). During the fall and winter, fish numbers varied with a peak on November 10 (1881 fish) and a minimum on December 7 (12 fish). A second, smaller, peak occurred on December 22 (607 fish). A univariate statistical analysis indicated fish abundance index (log10-transformed) was significantly (P<0.05) positively correlated with forebay elevation, velocity over the WTC tower intake gate weirs, and river flows into the reservoir. A subsequent multiple regression analysis resulted in a model (R2=0.70) predicting fish abundance (log-transformed index values) using two independent variables of mean forebay elevation and the log10 of the forebay elevation range. From the approximate fish length measurements made using the DIDSON imaging software, the average fish

  4. Evaluating ecological effects of small-scale agricultural diversions on stream flow in Coastal California

    NASA Astrophysics Data System (ADS)

    Deitch, M. J.; Kondolf, G. M.; Merenlender, A. M.

    2005-05-01

    In the absence of summer precipitation, grape growers in the California wine country often pump water directly from the stream for frost protection, irrigation, and heat protection. Managers may not be able to evaluate the impacts of the many small diversions on flow or anadromous salmonid habitat because of the uncertainty of human water needs. Building on previous research to predict diversion impacts on streamflow, we monitored flow in Franz Creek (a third-order stream draining 40 km2 in Sonoma County) to evaluate our model, and then measured water coverage in riffles and pools to quantify the change in salmonid habitat that these diversions would cause. Our model reasonably predicted flow decreases from diversion for frost protection: flow decreased by up to 80% on each cold morning, causing 50% riffle loss. Though it was not indicated in our model, the loss of habitat on hot summer days (suggesting diversion for heat protection) was also dramatic: levels dropped suddenly, reducing volume in intermittent pools by over 50%. Both changes in flow led to sudden reductions in habitat, suggesting that diversions are a major impediment to salmonid restoration in the region.

  5. Salmonid Gamete Preservation in the Snake River Basin, Annual Report 2002.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Young, William; Kucera, Paul

    2003-07-01

    In spite of an intensive management effort, chinook salmon (Oncorhynchus tshawytscha) and steelhead (Oncorhynchus mykiss) populations in the Northwest have not recovered and are currently listed as threatened species under the Endangered Species Act. In addition to the loss of diversity from stocks that have already gone extinct, decreased genetic diversity resulting from genetic drift and inbreeding is a major concern. Reduced population and genetic variability diminishes the environmental adaptability of individual species and entire ecological communities. The Nez Perce Tribe (NPT), in cooperation with Washington State University and the University of Idaho, established a germplasm repository in 1992 inmore » order to preserve the remaining salmonid diversity in the region. The germplasm repository provides long-term storage for cryopreserved gametes. Although only male gametes can be cryopreserved, conserving the male component of genetic diversity will maintain future management options for species recovery. NPT efforts have focused on preserving salmon and steelhead gametes from the major river subbasins in the Snake River basin. However, the repository is available for all management agencies to contribute gamete samples from other regions and species. In 2002 a total of 570 viable semen samples were added to the germplasm repository. This included the gametes of 287 chinook salmon from the Lostine River, Catherine Creek, upper Grande Ronde River, Imnaha River (Lookingglass Hatchery), Lake Creek, South Fork Salmon River, Johnson Creek, Big Creek, Capehorn Creek, Marsh Creek, Pahsimeroi River (Pahsimeroi Hatchery), and upper Salmon River (Sawtooth Hatchery) and the gametes of 280 steelhead from the North Fork Clearwater River (Dworshak Hatchery), Fish Creek, Little Sheep Creek, Pahsimeroi River (Pahsimeroi Hatchery) and Snake River (Oxbow Hatchery). In addition, gametes from 60 Yakima River spring chinook and 34 Wenatchee River coho salmon were added to the

  6. Salmonid Gamete Preservation in the Snake River Basin, 2001 Annual Report.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Armstrong, Robyn; Kucera, Paul

    2002-06-01

    Steelhead (Oncorhynchus mykiss) and chinook salmon (Oncorhynchus tshawytscha) populations in the Northwest are decreasing. Genetic diversity is being lost at an alarming rate. Along with reduced population and genetic variability, the loss of biodiversity means a diminished environmental adaptability. The Nez Perce Tribe (Tribe) strives to ensure availability of genetic samples of the existing male salmonid population by establishing and maintaining a germplasm repository. The sampling strategy, initiated in 1992, has been to collect and preserve male salmon and steelhead genetic diversity across the geographic landscape by sampling within the major river subbasins in the Snake River basin, assuming amore » metapopulation structure existed historically. Gamete cryopreservation conserves genetic diversity in a germplasm repository, but is not a recovery action for listed fish species. The Tribe was funded in 2001 by the Bonneville Power Administration (BPA) and the U.S. Fish and Wildlife Service Lower Snake River Compensation Plan (LSRCP) to coordinate gene banking of male gametes from Endangered Species Act (ESA) listed steelhead and spring and summer chinook salmon in the Snake River basin. In 2001, a total of 398 viable chinook salmon semen samples from the Lostine River, Catherine Creek, upper Grande Ronde River, Lookingglass Hatchery (Imnaha River stock), Lake Creek, the South Fork Salmon River weir, Johnson Creek, Big Creek, Capehorn Creek, Marsh Creek, Pahsimeroi Hatchery, and Sawtooth Hatchery (upper Salmon River stock) were cryopreserved. Also, 295 samples of male steelhead gametes from Dworshak Hatchery, Fish Creek, Grande Ronde River, Little Sheep Creek, Pahsimeroi Hatchery and Oxbow Hatchery were also cryopreserved. The Grande Ronde chinook salmon captive broodstock program stores 680 cryopreserved samples at the University of Idaho as a long-term archive, half of the total samples. A total of 3,206 cryopreserved samples from Snake River basin

  7. Habitat use by juvenile salmonids in Lake Ontario tributaries-species, age, diel and seasonal effects

    USGS Publications Warehouse

    Johnson, James H.; McKenna, James E.

    2017-01-01

    Understanding the habitat needs of fish and how these requirements may change seasonally over a 24-h period is important, especially for highly managed sport species. Consequently, we examined the diel and seasonal habitat use of four juvenile salmonid species in streams in the Lake Ontario watershed. For juvenile Atlantic salmon Salmo salarand juvenile rainbow trout Oncorhynchus mykiss, differences in day versus night habitat use were more profound than seasonal differences. Observed differences in day versus night habitat for all species and age classes were mainly due to the use of less object oriented cover at night and to a lesser extent to the use of slower velocities and smaller substrate at night. Seasonal differences in habitat use were also observed, likely due to increased fish size, and included movement to deeper and faster water and the use of larger substrate and more cover from summer to winter. Different habitat variables were important to individual species. Juvenile Atlantic salmon were associated with higher water velocities, juvenile rainbow trout with larger substrate and more cover, and subyearling Chinook salmon O. tshawytscha and subyearling coho salmon O. kisutch with small substrate and less cover. Our observations demonstrate that habitat partitioning occurs and likely reduces intraspecific and interspecific competition which may increase the potential production of all four species in sympatry. Consequently, these findings provide important information for resource managers charged with managing, protecting, and enhancing Great Lakes tributaries where all or some of these species occur.

  8. Molecular cloning and sequence analysis of the gene coding for the 57kDa soluble antigen of the salmonid fish pathogen Renibacterium salmoninarum

    USGS Publications Warehouse

    Chien, Maw-Sheng; Gilbert , Teresa L.; Huang, Chienjin; Landolt, Marsha L.; O'Hara, Patrick J.; Winton, James R.

    1992-01-01

    The complete sequence coding for the 57-kDa major soluble antigen of the salmonid fish pathogen, Renibacterium salmoninarum, was determined. The gene contained an opening reading frame of 1671 nucleotides coding for a protein of 557 amino acids with a calculated Mr value of 57190. The first 26 amino acids constituted a signal peptide. The deduced sequence for amino acid residues 27–61 was in agreement with the 35 N-terminal amino acid residues determined by microsequencing, suggesting the protein in synthesized as a 557-amino acid precursor and processed to produce a mature protein of Mr 54505. Two regions of the protein contained imperfect direct repeats. The first region contained two copies of an 81-residue repeat, the second contained five copies of an unrelated 25-residue repeat. Also, a perfect inverted repeat (including three in-frame UAA stop codons) was observed at the carboxyl-terminus of the gene.

  9. 15 CFR Supplement No. 8 to Part 742 - Self-Classification Report for Encryption Items

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... forensics (v) Cryptographic accelerator (vi) Data backup and recovery (vii) Database (viii) Disk/drive... (MAN) (xxii) Modem (xxiii) Network convergence or infrastructure n.e.s. (xxiv) Network forensics (xxv...

  10. 15 CFR Supplement No. 8 to Part 742 - Self-Classification Report for Encryption Items

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... forensics (v) Cryptographic accelerator (vi) Data backup and recovery (vii) Database (viii) Disk/drive... (MAN) (xxii) Modem (xxiii) Network convergence or infrastructure n.e.s. (xxiv) Network forensics (xxv...

  11. 15 CFR Supplement No. 8 to Part 742 - Self-Classification Report for Encryption Items

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... forensics (v) Cryptographic accelerator (vi) Data backup and recovery (vii) Database (viii) Disk/drive... (MAN) (xxii) Modem (xxiii) Network convergence or infrastructure n.e.s. (xxiv) Network forensics (xxv...

  12. 15 CFR Supplement No. 8 to Part 742 - Self-Classification Report for Encryption Items

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... forensics (v) Cryptographic accelerator (vi) Data backup and recovery (vii) Database (viii) Disk/drive... (MAN) (xxii) Modem (xxiii) Network convergence or infrastructure n.e.s. (xxiv) Network forensics (xxv...

  13. Sex Chromosome Evolution, Heterochiasmy, and Physiological QTL in the Salmonid Brook Charr Salvelinus fontinalis

    PubMed Central

    Sutherland, Ben J.G.; Rico, Ciro; Audet, Céline; Bernatchez, Louis

    2017-01-01

    Whole-genome duplication (WGD) can have large impacts on genome evolution, and much remains unknown about these impacts. This includes the mechanisms of coping with a duplicated sex determination system and whether this has an impact on increasing the diversity of sex determination mechanisms. Other impacts include sexual conflict, where alleles having different optimums in each sex can result in sequestration of genes into nonrecombining sex chromosomes. Sex chromosome development itself may involve sex-specific recombination rate (i.e., heterochiasmy), which is also poorly understood. The family Salmonidae is a model system for these phenomena, having undergone autotetraploidization and subsequent rediploidization in most of the genome at the base of the lineage. The salmonid master sex determining gene is known, and many species have nonhomologous sex chromosomes, putatively due to transposition of this gene. In this study, we identify the sex chromosome of Brook Charr Salvelinus fontinalis and compare sex chromosome identities across the lineage (eight species and four genera). Although nonhomology is frequent, homologous sex chromosomes and other consistencies are present in distantly related species, indicating probable convergence on specific sex and neo-sex chromosomes. We also characterize strong heterochiasmy with 2.7-fold more crossovers in maternal than paternal haplotypes with paternal crossovers biased to chromosome ends. When considering only rediploidized chromosomes, the overall heterochiasmy trend remains, although with only 1.9-fold more recombination in the female than the male. Y chromosome crossovers are restricted to a single end of the chromosome, and this chromosome contains a large interspecific inversion, although its status between males and females remains unknown. Finally, we identify quantitative trait loci (QTL) for 21 unique growth, reproductive, and stress-related phenotypes to improve knowledge of the genetic architecture of these

  14. A novel liquid medium for the efficient growth of the salmonid pathogen Piscirickettsia salmonis and optimization of culture conditions.

    PubMed

    Henríquez, Mirtha; González, Ernesto; Marshall, Sergio H; Henríquez, Vitalia; Gómez, Fernando A; Martínez, Irene; Altamirano, Claudia

    2013-01-01

    Piscirickettsia salmonis is the bacterium that causes Piscirickettsiosis, a systemic disease of salmonid fish responsible for significant economic losses within the aquaculture industry worldwide. The growth of the bacterium for vaccine formulation has been traditionally accomplished by infecting eukaryotic cell lines, a process that involves high production costs and is time-consuming. Recent research has demonstrated that it is possible to culture pure P. salmonis in a blood containing (cell-free) medium. In the present work we demonstrate the growth of P. salmonis in a liquid medium free from blood and serum components, thus establishing a novel and simplified bacteriological medium. Additionally, the new media reported provides improved growth conditions for P. salmonis, where biomass concentrations of approximately 800 mg cell dry weight L(-1) were obtained, about eight times higher than those reported for the blood containing medium. A 2- level full factorial design was employed to evaluate the significance of the main medium components on cell growth and an optimal temperature range of 23-27°C was determined for the microorganism to grow in the novel liquid media. Therefore, these results represent a breakthrough regarding P. salmonis research in order to optimize pure P. salmonis growth in liquid blood and serum free medium.

  15. Differences in detection of Aeromonas salmonicida in covertly infected salmonid fishes by the stress-inducible furunculosis test and culture-based assays

    USGS Publications Warehouse

    Cipriano, R.C.; Ford, L.A.; Smith, D.R.; Schachte, J.H.; Petrie, C.J.

    1997-01-01

    Accurate detection of Aeromonas salmonicida subsp. salmonicida (the cause of furunculosis disease) in covertly infected salmonids is difficult and is a cause of concern for those involved in fish health inspection and resource management programs. In this study, we examined populations of brook trout Salvelinus fontinalis, Atlantic salmon Salmo salar, and lake trout Salvelinus namaycush that previously sustained natural episodes of furunculosis. Consequently, the sampled fish were presumed to harbor latent infections. Mucus, gill, liver, kidney, heart, spleen, and intestine samples (N = 100 fish per group sampled) were processed and examined by (1) direct dilution counts and (2) quadrant streaking after a 48-h pre-enrichment in trypticase soy broth (TSB). Another subsample of fish from each group was then subjected to stress-inducible furunculosis tests. Stress tests detected A. salmonicida in three of four groups of fish that were examined whereas the pathogen was detected in only two of the groups analyzed with culture-based assays. Although pre-enrichment in TSB enhanced detection within internal sampling sites including the liver, heart, spleen, and kidney, enrichment did not enhance detection from mucus, gill, or intestinal samples.

  16. Incorporating spatial context into the analysis of salmonid-habitat relationships

    EPA Science Inventory

    In this response to the chapter by Lapointe (this volume), we discuss the question of why it is so difficult to predict salmonid–habitat relationships in gravel-bed rivers and streams. We acknowledge that this cannot be an exhaustive treatment of the subject and, thus, identify ...

  17. Small-Volume Injections: Evaluation of Volume Administration Deviation From Intended Injection Volumes.

    PubMed

    Muffly, Matthew K; Chen, Michael I; Claure, Rebecca E; Drover, David R; Efron, Bradley; Fitch, William L; Hammer, Gregory B

    2017-10-01

    In the perioperative period, anesthesiologists and postanesthesia care unit (PACU) nurses routinely prepare and administer small-volume IV injections, yet the accuracy of delivered medication volumes in this setting has not been described. In this ex vivo study, we sought to characterize the degree to which small-volume injections (≤0.5 mL) deviated from the intended injection volumes among a group of pediatric anesthesiologists and pediatric postanesthesia care unit (PACU) nurses. We hypothesized that as the intended injection volumes decreased, the deviation from those intended injection volumes would increase. Ten attending pediatric anesthesiologists and 10 pediatric PACU nurses each performed a series of 10 injections into a simulated patient IV setup. Practitioners used separate 1-mL tuberculin syringes with removable 18-gauge needles (Becton-Dickinson & Company, Franklin Lakes, NJ) to aspirate 5 different volumes (0.025, 0.05, 0.1, 0.25, and 0.5 mL) of 0.25 mM Lucifer Yellow (LY) fluorescent dye constituted in saline (Sigma Aldrich, St. Louis, MO) from a rubber-stoppered vial. Each participant then injected the specified volume of LY fluorescent dye via a 3-way stopcock into IV tubing with free-flowing 0.9% sodium chloride (10 mL/min). The injected volume of LY fluorescent dye and 0.9% sodium chloride then drained into a collection vial for laboratory analysis. Microplate fluorescence wavelength detection (Infinite M1000; Tecan, Mannedorf, Switzerland) was used to measure the fluorescence of the collected fluid. Administered injection volumes were calculated based on the fluorescence of the collected fluid using a calibration curve of known LY volumes and associated fluorescence.To determine whether deviation of the administered volumes from the intended injection volumes increased at lower injection volumes, we compared the proportional injection volume error (loge [administered volume/intended volume]) for each of the 5 injection volumes using a linear

  18. Prevalence of piscine orthoreovirus and salmonid alphavirus in sea-caught returning adult Atlantic salmon (Salmo salar L.) in northern Norway.

    PubMed

    Madhun, A S; Isachsen, C H; Omdal, L M; Einen, A C B; Maehle, S; Wennevik, V; Niemelä, E; Svåsand, T; Karlsbakk, E

    2018-05-01

    Heart and skeletal muscle inflammation (HSMI) caused by piscine orthoreovirus (PRV) and pancreas disease (PD) caused by salmonid alphavirus (SAV) are among the most prevalent viral diseases of Atlantic salmon farmed in Norway. There are limited data about the impact of disease in farmed salmon on wild salmon populations. Therefore, the prevalence of PRV and SAV in returning salmon caught in six sea sites was determined using real-time RT-PCR analyses. Of 419 salmon tested, 15.8% tested positive for PRV, while none were positive for SAV. However, scale reading revealed that 10% of the salmon had escaped from farms. The prevalence of PRV in wild salmon (8%) was significantly lower than in farm escapees (86%), and increased with fish length (proxy for age). Sequencing of the S1 gene of PRV from 39 infected fish revealed a mix of genotypes. The observed increase in PRV prevalence with fish age and the lack of phylogeographic structure of the virus could be explained by virus transmission in the feeding areas. Our results highlight the need for studies about the prevalence of PRV and other pathogens in Atlantic salmon in its oceanic phase. © 2018 The Authors. Journal of Fish Diseases Published by John Wiley & Sons Ltd.

  19. A comparative evaluation of crowding stress on muscle HSP90 and myostatin expression in salmonids

    USGS Publications Warehouse

    Galt, Nicholas J.; Froehlich, Jacob Michael; McCormick, Stephen; Biga, Peggy R.

    2018-01-01

    Stress is a major factor that contributes to poor production and animal welfare concerns in aquaculture. As such, a thorough understanding of mechanisms involved in the stress response is imperative to developing strategies to mitigate the negative side effects of stressors, including the impact of high stocking densities on growth. The purpose of this study was to determine how the muscle growth inhibitor, myostatin, and the stress-responsive gene HSP90 are regulated in response to crowding stress in rainbow trout (Oncorhynchus mykiss), cutthroat trout (Oncorhynchus clarki), brook trout (Salvelinus fontinalis), and Atlantic salmon (Salmo salar). All species exhibited higher cortisol and glucose levels following the handling stress, indicating physiological response to the treatment. Additionally, all species, except rainbow trout, exhibited higher HSP90 levels in muscle after a 48 h crowding stress. Crowding stress resulted in a decrease of myostatin-1ain brook trout white muscle but not red muscle, while, myostatin-1a and -2a levels increased in white muscle and myostatin-1b levels increased in red muscle in Atlantic salmon. In rainbow trout, no significant changes were detected in either muscle type, but myostatin-1awas upregulated in both white and red skeletal muscle in the closely related cutthroat trout. The variation in response to crowding suggests a complex and species-specific interaction between stress and the muscle gene regulation in these salmonids. Only Atlantic salmon and cutthroat trout exhibited increased muscle myostatin transcription, and also exhibited the largest increase in circulating glucose in response to crowding. These results suggest that species-specific farming practices should be carefully examined in order to optimize low stress culture conditions.

  20. Comparative Performance of Acoustic-tagged and PIT-tagged Juvenile Salmonids

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hockersmith, Eric E.; Brown, Richard S.; Liedtke, Theresa L.

    2008-02-01

    Numerous research tools and technologies are currently being used to evaluate fish passage and survival to determine the impacts of the Federal Columbia River Power System (FCRPS) on endangered and threatened juvenile salmonids, including PIT tags, balloon tags, hydroacoustic evaluations, radio telemetry, and acoustic telemetry. Each has advantages and disadvantages, but options are restricted in some situations because of limited capabilities of a specific technology, lack of detection capability downstream, or availability of adequate numbers of fish. However, there remains concern about the comparative effects of the tag or the tagging procedure on fish performance. The recently developed Juvenile Salmonidmore » Acoustic Telemetry System (JSATS) acoustic transmitter is the smallest active acoustic tag currently available. The goal of this study was to determine whether fish tagged with the JSATS acoustic-telemetry tag can provide unbiased estimates of passage behavior and survival within the performance life of the tag. We conducted both field and laboratory studies to assess tag effects. For the field evaluation we released a total of 996 acoustic-tagged fish in conjunction with 21,026 PIT-tagged fish into the tailrace of Lower Granite Dam on 6 and 13 May. Travel times between release and downstream dams were not significantly different for the majority of the reaches between acoustic-tagged and PIT-tagged fish. In addition to the field evaluation, a series of laboratory experiments were conducted to determine if growth and survival of juvenile Chinook salmon surgically implanted with acoustic transmitters is different than untagged or PIT tagged juvenile Chinook salmon. Only yearling fish with integrated and non-integrated transmitters experienced mortalities, and these were low (<4.5%). Mortality among sub-yearling control and PIT-tag treatments ranged up to 7.7% while integrated and non-integrated treatments had slightly higher rates (up to 8.3% and 7

  1. [Target volume margins for lung cancer: internal target volume/clinical target volume].

    PubMed

    Jouin, A; Pourel, N

    2013-10-01

    The aim of this study was to carry out a review of margins that should be used for the delineation of target volumes in lung cancer, with a focus on margins from gross tumour volume (GTV) to clinical target volume (CTV) and internal target volume (ITV) delineation. Our review was based on a PubMed literature search with, as a cornerstone, the 2010 European Organisation for Research and Treatment of Cancer (EORTC) recommandations by De Ruysscher et al. The keywords used for the search were: radiotherapy, lung cancer, clinical target volume, internal target volume. The relevant information was categorized under the following headings: gross tumour volume definition (GTV), CTV-GTV margin (first tumoural CTV then nodal CTV definition), in field versus elective nodal irradiation, metabolic imaging role through the input of the PET scanner for tumour target volume and limitations of PET-CT imaging for nodal target volume definition, postoperative radiotherapy target volume definition, delineation of target volumes after induction chemotherapy; then the internal target volume is specified as well as tumoural mobility for lung cancer and respiratory gating techniques. Finally, a chapter is dedicated to planning target volume definition and another to small cell lung cancer. For each heading, the most relevant and recent clinical trials and publications are mentioned. Copyright © 2013. Published by Elsevier SAS.

  2. Long term retention, survival, growth, and physiological indicators of salmonids marked with passive integrated transponder tags

    USGS Publications Warehouse

    Ostrand, Kenneth G.; Zydlewski, Gayle B.; Gale, William L.; Zydlewski, Joseph D.

    2011-01-01

    To track individuals in situ, over 12 million salmon and trout have been marked with passive integrated transponder (PIT) tags in the Columbia River Basin, USA. However, few studies have examined long term tag retention as well as tag effects on juvenile salmon and trout. We marked juvenile coho salmon Oncorhynchus kisutch (N = 207), steelhead (anadromous rainbow trout) O. mykiss (N = 221), cutthroat trout O. clarkii (N = 202) and bull trout Salvelinus confluentus (N = 180) with 12, 19, or 23 mm PIT tags and examined tag retention, survival, growth, and physiological performance over a six month period in a laboratory environment. PIT tag retention rates were high for coho salmon (100%), steelhead (95%), cutthroat trout (97%), and bull trout (99%), regardless of tag size. Survival was also high for coho (99%), steelhead (99%), cutthroat trout (97%), and bull trout (88%) and did not vary among tag sizes. Short term individual growth rates for coho salmon marked with 12 mm tags were significantly higher than those marked with 19 mm and 23 mm PIT tags. Likewise, steelhead trout individual growth rates were lower for fish marked with 23 mm PIT tags followed by 19 and 12 mm tags. Conversely, long-term growth rates were positive and not affected by tag size. There were no significant effects of tag size or marking on coho gill Na+, K+, -ATPase activity (µmol ADP x mg protein–1 h–1) and plasma osmolality (µmol kg–1) or bull trout hepatosomatic indices. Our study suggests that marking juvenile salmonids with PIT tags results in high retention with little effect upon their survival, growth, and important physiological indicators regardless of tag size in a laboratory environment.

  3. Criteria for reducing predation by northern squawfish near juvenile salmonid bypass outfalls at Columbia River dams

    USGS Publications Warehouse

    Shively, Rip S.; Poe, Thomas P.; Sheer, Mindi B.; Peters, Rock

    1996-01-01

    Predation by northern squawfish (Ptychocheilus oregonensis) has been documented to be significant on emigrating juvenile salmonids near juvenile bypass outfalls at hydroelectric dams on the Columbia River. Criteria for siting juvenile fish bypass outfalls to reduce predation were developed using locational data from radio-tagged northern squawfish in The Dalles Dam trailrace, Columbia River. Radio transmitters were surgically implanted in 164 northern squawfish in 1993 and 1994, and their movements and distribution were monitored. Position estimates of northern squawfish were compared with data from a physical hydraulic model of the dam to estimate water velocities where northern squawfish were located. Eighty-two percent of northern squawfish position estimates were in water velocities ≤110 cm/s in 1993 and ≤90 cm/s in 1994. Fish locations were usually associated with water depths ≤10 m (84% in 1993 and 82% in 1994); 90% were within 110 m of the shore or dam structure in 1993, and 86% were within 80 m in 1994. In a related study at John Day Dam, Columbia River, where the juvenile bypass outfall is located 40 m from shore, water depth is 10 m and water velocities typically exceed 75 cm/s, only 13 of 1443 (0.9%) contacts on radio-tagged northern squawfish were located within 200 m of the bypass outfall. We recommend that new or modified juvenile bypass outfalls on the Columbia River be located in water velocities of ≥100 cm/s, ≥75 m from the shore or dam structure, and in water ≥10 m deep.

  4. How Tight is the Linkage Between Trees and Trout?

    Treesearch

    Margaret A. Wilzbach

    1989-01-01

    This paper explores the tightness of the linkage between stream-dwelling salmonids and ripar ian vegetation. Comparison of original distributions of salmonid species with that of vegetation types shows that distribution within a given salmonid species is not limited to a specific vegetation type, and that different salmonid species cooccur within a given vegetation...

  5. Perturbation in protein expression of the sterile salmonid hybrids between female brook trout Salvelinus fontinalis and male masu salmon Oncorhynchus masou during early spermatogenesis.

    PubMed

    Zheng, Liang; Senda, Yoshie; Abe, Syuiti

    2013-05-01

    Most males and females of intergeneric hybrid (BM) between female brook trout (Bt) Salvelinus fontinalis and male masu salmon (Ms) Oncorhynchus masou had undeveloped gonads, with abnormal germ cell development shown by histological examination. To understand the cause of this hybrid sterility, expression profiles of testicular proteins in the BM and parental species were examined with 2-DE coupled with MALDI-TOF/TOF MS. Compared with the parental species, more than 60% of differentially expressed protein spots were down-regulated in BM. A total of 16 up-regulated and 48 down-regulated proteins were identified in BM. Up-regulated were transferrin and other somatic cell-predominant proteins, whereas down-regulated were some germ cell-specific proteins such as DEAD box RNA helicase Vasa. Other pronouncedly down-regulated proteins included tubulins and heat shock proteins that are supposed to have roles in spermatogenesis. The present findings suggest direct association of the observed perturbation in protein expression with the failure of spermatogenesis and the sterility in the examined salmonid hybrids. Copyright © 2013 Elsevier B.V. All rights reserved.

  6. 8 CFR 241.2 - Warrant of removal.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...: (i) Director, Detention and Removal Operations; (ii) Deputy Assistant Director, Field Operations... Directors; (xxi) Deputy Port Directors; (xxii) Assistant Port Directors; (xxiii) Director, Field Operations; (xxiv) Deputy Director, Field Operations; (xxv) Assistant Director, Field Operations; and (xxvi) Other...

  7. Summary of juvenile salmonid passage and survival at McNary Dam-Acoustic survival studies, 2006-09

    USGS Publications Warehouse

    Adams, Noah S.; Evans, Scott D.

    2011-01-01

    Passage and survival data were collected at McNary Dam between 2006 and 2009. These data have provided critical information for resource managers to implement structural and operational changes designed to improve the survival of juvenile salmonids as they migrate past the dam. Given the importance of these annual studies, the primary objectives of this report were to summarize the findings of these annual studies to ensure that passage and survival metrics are consistently calculated and reported across all years and to consolidate this information in a single document, thereby making it easier to reference. It is worth noting that this report does not contain all the information from all the annual reports. The intent of this report was to summarize the key findings from multiple years of research. The reader is encouraged to reference the annual reports if more detailed information is needed. Chapter 1 summarizes existing behavior, passage, and survival results for fish released 10 rkm upstream of McNary Dam and from the McNary Dam tailrace during 2006-09. Chapter 2 summarizes existing behavior, passage, and survival results for fish released in the mid-Columbia River and detected at McNary Dam during 2006-09. Results from 2006 indicated that higher spill discharge generally resulted in higher fish passage through spill, and in turn, higher fish survival through the entire dam. Within the spillway, passage effectiveness was highest for the south spill bays, adjacent to the powerhouse. Increased passage in this area, combined with detailed 3-dimensional approach paths, aided in the design and location of the temporary spillway weirs (TSWs) at McNary Dam prior to the 2007 migration of juvenile salmonids. During the 2007 study, the TSWs were tested under two spill treatments during the spring and summer: a "2006 Modified spill," and a "2007 test spill." In the spring, slightly higher discharge through spill bays 14-17 was the primary difference between the spill

  8. Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3).

    PubMed

    Braceland, M; Tinsley, J; Cockerill, D; Bickerdike, R; McLoughlin, M F; Eckersall, P D

    2017-08-01

    While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on-farm qualitative test and an in-laboratory quantitative assay for health assessments in aquaculture. Mixing serum from Atlantic salmon, Salmo salar, with SAV infection with a sodium acetate buffer caused a visible precipitation which does not occur with serum from healthy salmon. Proteomic examination of the precipitate has revealed that the components are a mix of muscle proteins, for example enolase and aldolase, along with serum protein such as serotransferrin and complement C9. The assay has been optimized for molarity, pH, temperature and wavelength so that the precipitation can be measured as the change in optical density at 340 nm (Δ 340 ). Application of the SPR assay to serum samples from a cohabitation trial of SAV infection in salmon showed that the Δ 340 in infected fish rose from undetectable to a maximum at 6 weeks post-infection correlating with histopathological score of pancreas, heart and muscle damage. This test may have a valuable role to play in the diagnostic evaluation of stock health in salmon. © 2016 The Authors. Journal of Fish Diseases Published by John Wiley & Sons Ltd.

  9. Local habitat conditions explain the variation in the strength of self-thinning in a stream salmonid

    PubMed Central

    Myrvold, Knut Marius; Kennedy, Brian P

    2015-01-01

    Self-thinning patterns are frequently used to describe density dependence in populations on timescales shorter than the organism's life span and have been used to infer carrying capacity of the environment. Among mobile animals, this concept has been used to document density dependence in stream salmonids, which compete over access to food and space. The carrying capacity, growth conditions, and initial cohort sizes often vary between streams and stream sections, which would influence the onset and strength of the density dependence. Despite much effort in describing habitat relationships in stream fishes, few studies have explicitly tested how the physical environment affects the slope of the thinning curves. Here, we investigate the prevalence and strength of self-thinning in juvenile stages of a steelhead (Oncorhynchus mykiss) population in Idaho, USA. Further, we investigate the roles of local physical habitat and metabolic constraints in explaining the variation in thinning curves among study sites in the watershed. Only yearling steelhead exhibited an overall significant thinning trend, but the slope of the mass–density relationship (−0.53) was shallower than predicted by theory and reported from empirical studies. There was no detectable relationship in subyearling steelhead. Certain abiotic factors explained a relatively large portion of the variation in the strength of the self-thinning among the study reaches. For subyearling steelhead, the slopes were negatively associated with the average water depth and flow velocity in the study sites, whereas slopes in yearlings were steeper in sites that incurred a higher metabolic cost. Our results show that the prevalence and strength of density dependence in natural fish populations can vary across heterogeneous watersheds and can be more pronounced during certain stages of a species' life history, and that environmental factors can mediate the extent to which density dependence is manifested in predictable

  10. Laboratory calibration of density-dependent lines in the extreme ultraviolet spectral region

    NASA Astrophysics Data System (ADS)

    Lepson, J. K.; Beiersdorfer, P.; Gu, M. F.; Desai, P.; Bitter, M.; Roquemore, L.; Reinke, M. L.

    2012-05-01

    We have been making spectral measurements in the extreme ultraviolet (EUV) from different laboratory sources in order to investigate the electron density dependence of various astrophysically important emission lines and to test the atomic models underlying the diagnostic line ratios. The measurement are being performed at the Livermore EBIT-I electron beam ion trap, the National Spherical Torus Experiment (NSTX) at Princeton, and the Alcator C-Mod tokamak at the Massachusetts Institute of Technology, which together span an electron density of four orders of magnitude and which allow us to test the various models at high and low density limits. Here we present measurements of Fe XXII and Ar XIV, which include new data from an ultra high resolution (λ/Δλ >4000) spectrometer at the EBIT-I facility. We found good agreement between the measurements and modeling calculations for Fe XXII, but poorer agreement for Ar XIV.

  11. Bioinvasive species and the preservation of cutthroat trout in the western United States: Ecological, social, and economic issues

    USGS Publications Warehouse

    Quist, M.C.; Hubert, W.A.

    2004-01-01

    The cutthroat trout (Oncorhynchus clarki) was the only endemic salmonid species across most of the western United States, and it has severely declined largely due to introduction and bioinvasion by non-native salmonid species. However, the ecological, social, and economic consequences of cutthroat trout declines and replacement by non-native salmonid species are relatively minor, and measurable affects on ecosystem function are rare. Restoration efforts for cutthroat trout involve removal or control of bioinvasive salmonid species, but such efforts are costly, ongoing, and resisted frequently by segments of society. Cutthroat trout declines are of little concern to much of the public because they are valued similarly to non-native salmonids, and non-native salmonid species frequently have higher recreational values. Due to the low values placed on cutthroat trout relative to non-native salmonid species, net economic benefits of preserving cutthroat trout are equal to or less than those for non-native salmonids. Cutthroat trout provide a classic case of the consequences of biological invasion; however, other native species are faced with similar issues. We suggest that management agencies establish realistic goals to preserve native species within the context of ecological, social, and economic issues. ?? 2004 Elsevier Ltd. All rights reserved.

  12. Impact of Salmonid alphavirus infection in diploid and triploid Atlantic salmon (Salmo salar L.) fry.

    PubMed

    Herath, Tharangani K; Ashby, Angela J; Jayasuriya, Nilantha S; Bron, James E; Taylor, John F; Adams, Alexandra; Richards, Randolph H; Weidmann, Manfred; Ferguson, Hugh W; Taggart, John B; Migaud, Herve; Fordyce, Mark J; Thompson, Kim D

    2017-01-01

    With increasing interest in the use of triploid salmon in commercial aquaculture, gaining an understanding of how economically important pathogens affect triploid stocks is important. To compare the susceptibility of diploid and triploid Atlantic salmon (Salmo salar L.) to viral pathogens, fry were experimentally infected with Salmonid alphavirus sub-type 1 (SAV1), the aetiological agent of pancreas disease (PD) affecting Atlantic salmon aquaculture in Europe. Three groups of fry were exposed to the virus via different routes of infection: intraperitoneal injection (IP), bath immersion, or cohabitation (co-hab) and untreated fry were used as a control group. Mortalities commenced in the co-hab challenged diploid and triploid fish from 11 days post infection (dpi), and the experiment was terminated at 17 dpi. Both diploid and triploid IP challenged groups had similar levels of cumulative mortality at the end of the experimental period (41.1% and 38.9% respectively), and these were significantly higher (p < 0.01) than for the other challenge routes. A TaqMan-based quantitative PCR was used to assess SAV load in the heart, a main target organ of the virus, and also liver, which does not normally display any pathological changes during clinical infections, but exhibited severe degenerative lesions in the present study. The median viral RNA copy number was higher in diploid fish compared to triploid fish in both the heart and the liver of all three challenged groups. However, a significant statistical difference (p < 0.05) was only apparent in the liver of the co-hab groups. Diploid fry also displayed significantly higher levels of pancreatic and myocardial degeneration than triploids. This study showed that both diploid and triploid fry are susceptible to experimental SAV1 infection. The lower virus load seen in the triploids compared to the diploids may possibly be related to differences in cell metabolism between the two groups, however, further investigation is

  13. Impact of Salmonid alphavirus infection in diploid and triploid Atlantic salmon (Salmo salar L.) fry

    PubMed Central

    Ashby, Angela J.; Jayasuriya, Nilantha S.; Bron, James E.; Taylor, John F.; Adams, Alexandra; Richards, Randolph H.; Weidmann, Manfred; Ferguson, Hugh W.; Taggart, John B.; Migaud, Herve; Fordyce, Mark J.; Thompson, Kim D.

    2017-01-01

    With increasing interest in the use of triploid salmon in commercial aquaculture, gaining an understanding of how economically important pathogens affect triploid stocks is important. To compare the susceptibility of diploid and triploid Atlantic salmon (Salmo salar L.) to viral pathogens, fry were experimentally infected with Salmonid alphavirus sub-type 1 (SAV1), the aetiological agent of pancreas disease (PD) affecting Atlantic salmon aquaculture in Europe. Three groups of fry were exposed to the virus via different routes of infection: intraperitoneal injection (IP), bath immersion, or cohabitation (co-hab) and untreated fry were used as a control group. Mortalities commenced in the co-hab challenged diploid and triploid fish from 11 days post infection (dpi), and the experiment was terminated at 17 dpi. Both diploid and triploid IP challenged groups had similar levels of cumulative mortality at the end of the experimental period (41.1% and 38.9% respectively), and these were significantly higher (p < 0.01) than for the other challenge routes. A TaqMan-based quantitative PCR was used to assess SAV load in the heart, a main target organ of the virus, and also liver, which does not normally display any pathological changes during clinical infections, but exhibited severe degenerative lesions in the present study. The median viral RNA copy number was higher in diploid fish compared to triploid fish in both the heart and the liver of all three challenged groups. However, a significant statistical difference (p < 0.05) was only apparent in the liver of the co-hab groups. Diploid fry also displayed significantly higher levels of pancreatic and myocardial degeneration than triploids. This study showed that both diploid and triploid fry are susceptible to experimental SAV1 infection. The lower virus load seen in the triploids compared to the diploids may possibly be related to differences in cell metabolism between the two groups, however, further investigation is

  14. 40 CFR 70.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...: (i) Coal cleaning plants (with thermal dryers); (ii) Kraft pulp mills; (iii) Portland cement plants... plants; (xii) Phosphate rock processing plants; (xiii) Coke oven batteries; (xiv) Sulfur recovery plants...) totaling more than 250 million British thermal units per hour heat input; (xxii) Petroleum storage and...

  15. 40 CFR 71.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...: (i) Coal cleaning plants (with thermal dryers); (ii) Kraft pulp mills; (iii) Portland cement plants... plants; (xii) Phosphate rock processing plants; (xiii) Coke oven batteries; (xiv) Sulfur recovery plants...) totaling more than 250 million British thermal units per hour heat input; (xxii) Petroleum storage and...

  16. 77 FR 74341 - Establishing the Hurricane Sandy Rebuilding Task Force

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-14

    ... Economic Advisers; (xxii) the White House Office of Public Engagement and Intergovernmental Affairs; (xxiii... their official capacity, may designate employees with authority to act on their behalf. The Advisory... Advisory Group members and elected officials, or their designated employees, as necessary to provide advice...

  17. Juvenile Salmonid survival, passage, and egress at McNary Dam during tests of temporary spillway weirs, 2009

    USGS Publications Warehouse

    Adams, N.S.; Liedtke, T.L.

    2010-01-01

    The TSWs proved to be a relatively effective way to pass juvenile salmonids at McNary Dam (Summary Tables 1.1, 1.2, and 1.3), as was the case in 2007 and 2008. The TSWs passed about 14% of yearling Chinook salmon and 34% of juvenile steelhead with only 5-10% of total project discharge flowing through the TSWs. The TSWs and adjacent spill bays 16-18 passed 27% of subyearling Chinook salmon in the summer with 6-16% of total project discharge flowing through the TSWs. Based on the number of fish passing per the proportion of water flowing through the spillway (i.e., passage effectiveness), the TSWs were the most effective passage route. Passage effectiveness for fish passing through both TSW structures was 2.0 for yearling Chinook salmon, 5.2 for juvenile steelhead, and 2.7 subyearling Chinook salmon for TSW 20 alone. Higher passage of juvenile steelhead through the TSWs could have resulted from juvenile steelhead being more surface-oriented during migration (Plumb et al. 2004; Beeman et al. 2007; Beeman and Maule 2006). Based on passage performance and effectiveness metrics, TSW 4, located on the north end of the spillway, did not perform as well as TSW 20, located on the south end of the spillway. Passage proportions for TSW 4 were at least half that of the levels observed for TSW 20 for both yearling Chinook salmon and juvenile steelhead. This difference may be attributed to TSW location or other variables such as dam operations. Regardless of which TSW was used by fish passing the dam, survival through both TSWs was high (> 0.98 for paired-release dam survival) for yearling Chinook salmon and juvenile steelhead.

  18. Wyoming Low-Volume Roads Traffic Volume Estimation

    DOT National Transportation Integrated Search

    2015-10-01

    Low-volume roads are excluded from regular traffic counts except on a need to know basis. But needs for traffic volume data on low-volume roads in road infrastructure management, safety, and air quality analysis have necessitated regular traffic volu...

  19. Measuring pedestrian volumes and conflicts. Volume 1, Pedestrian volume sampling

    DOT National Transportation Integrated Search

    1987-12-01

    This final report presents the findings, conclusions, and recommendations of the study conducted to develop a model to predict pedestrian volumes using small sampling schemes. This research produced four pedestrian volume prediction models (i.e., 1-,...

  20. Salmonid Gamete Preservation in the Snake River Basin : 2000 Annual Report.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Armstrong, Robyn; Kucera, Paul A.

    2001-06-01

    Steelhead (Oncorhynchus mykiss) and chinook salmon (Oncorhynchus tshawytscha) populations in the Northwest are decreasing. Genetic diversity is being lost at an alarming rate. The Nez Perce Tribe (Tribe) strives to ensure availability of genetic samples of the existing male salmonid population by establishing and maintaining a germplasm repository. The sampling strategy, initiated in 1992, has been to collect and preserve male salmon and steelhead genetic diversity across the geographic landscape by sampling within the major river subbasins in the Snake River basin, assuming a metapopulation structure existed historically. Gamete cryopreservation conserves genetic diversity in a germplasm repository, but is notmore » a recovery action for listed fish species. The Tribe was funded in 2000 by the Bonneville Power Administration (BPA) and the U.S. Fish and Wildlife Service Lower Snake River Compensation Plan (LSRCP) to coordinate gene banking of male gametes from Endangered Species Act listed steelhead and spring and summer chinook salmon in the Snake River basin. In 2000, a total of 349 viable chinook salmon semen samples from the Lostine River, Catherine Creek, upper Grande Ronde River, Lookingglass Hatchery (Imnaha River stock), Rapid River Hatchery, Lake Creek, the South Fork Salmon River weir, Johnson Creek, Big Creek, Capehorn Creek, Marsh Creek, Pahsimeroi Hatchery, and Sawtooth Hatchery (upper Salmon River stock) were cryopreserved. Also, 283 samples of male steelhead gametes from Dworshak Hatchery, Fish Creek, Grande Ronde River, Imnaha River, Little Sheep Creek, Pahsimeroi Hatchery and Oxbow Hatchery were also cryopreserved. The Tribe acquired 5 frozen steelhead samples from the Selway River collected in 1994 and 15 from Fish Creek sampled in 1993 from the U.S. Geological Survey, for addition into the germplasm repository. Also, 590 cryopreserved samples from the Grande Ronde chinook salmon captive broodstock program are being stored at the University of

  1. When high-volume PCI operators in high-volume hospitals move to lower volume hospitals-Do they still maintain high volume and quality of outcomes?

    PubMed

    Lu, Tsung-Hsueh; Li, Sheng-Tun; Liang, Fu-Wen; Lee, Jo-Chi; Yin, Wei-Hsian

    2017-10-31

    The aim of this quasi-experimental study was to examine whether high-volume percutaneous coronary intervention (PCI) operators still maintain high volume and quality of outcomes when they moved to lower volume hospitals. Systematic reviews have indicated that high-volume PCI operators and hospitals have higher quality outcomes. However, little is known on whether high PCI volume and high quality outcomes are mainly due to operator characteristics (i.e., skill and experience) and is portable across organizations or whether it is due to hospital characteristics (i.e., equipment, team, and management system) and is less portable. We used Taiwan National Health Insurance claims data 2000-2012 to identify 98 high-volume PCI operators, 10 of whom moved from one hospital to another during the study period. We compared the PCI volume, risk-adjusted mortality ratio, and major adverse cardiovascular event (MACE) ratio before and after moving. Of the 10 high-volume operators who moved, 6 moved from high- to moderate- or low-volume hospitals, with median annual PCI volumes (interquartile range) of 130 (117-165) in prior hospitals and 54 (46-84) in subsequent hospitals (the hospital the operator moved to), and the remaining 4 moved from high to high-volume hospitals, with median annual PCI volumes (interquartile range) of 151 (133-162) in prior hospitals and 193 (178-239) in subsequent hospitals. No significant differences were observed in the risk-adjusted mortality ratios and MACE ratios between high-volume operators and matched controls before and after moving. High-volume operators cannot maintain high volume when they moved from high to moderate or low-volume hospitals; however, the quality of care is maintained. High PCI volume and high-quality outcomes are less portable and more hospital bound. © 2017 Wiley Periodicals, Inc.

  2. Distribution and Fate of Energetics on DoD Test and Training Ranges

    DTIC Science & Technology

    2004-11-01

    766 ND ND ND 1,2,4-Tdchlorobenzene pg/L 0 954 ND ND ND 1,2-Dibromo-3- Chloropropane pg/L 1 766 0.60 0.60 0.60 1,2-Dibromoethane (Ethylene Dibromide) pg...xxii 1 - Introduction .................................................................................................. 1 - 1 Background... 1 - 1 Scope of Project CP 1155

  3. Measurement of limb volume: laser scanning versus volume displacement.

    PubMed

    McKinnon, John Gregory; Wong, Vanessa; Temple, Walley J; Galbraith, Callum; Ferry, Paul; Clynch, George S; Clynch, Colin

    2007-10-01

    Determining the prevalence and treatment success of surgical lymphedema requires accurate and reproducible measurement. A new method of measurement of limb volume is described. A series of inanimate objects of known and unknown volume was measured using digital laser scanning and water displacement. A similar comparison was made with 10 human volunteers. Digital scanning was evaluated by comparison to the established method of water displacement, then to itself to determine reproducibility of measurement. (1) Objects of known volume: Laser scanning accurately measured the calculated volume but water displacement became less accurate as the size of the object increased. (2) Objects of unknown volume: As average volume increased, there was an increasing bias of underestimation of volume by the water displacement method. The coefficient of reproducibility of water displacement was 83.44 ml. In contrast, the reproducibility of the digital scanning method was 19.0 ml. (3) Human data: The mean difference between water displacement volume and laser scanning volume was 151.7 ml (SD +/- 189.5). The coefficient of reproducibility of water displacement was 450.8 ml whereas for laser scanning it was 174 ml. Laser scanning is an innovative method of measuring tissue volume that combines precision and reproducibility and may have clinical utility for measuring lymphedema. 2007 Wiley-Liss, Inc

  4. Sparse PDF Volumes for Consistent Multi-Resolution Volume Rendering.

    PubMed

    Sicat, Ronell; Krüger, Jens; Möller, Torsten; Hadwiger, Markus

    2014-12-01

    This paper presents a new multi-resolution volume representation called sparse pdf volumes, which enables consistent multi-resolution volume rendering based on probability density functions (pdfs) of voxel neighborhoods. These pdfs are defined in the 4D domain jointly comprising the 3D volume and its 1D intensity range. Crucially, the computation of sparse pdf volumes exploits data coherence in 4D, resulting in a sparse representation with surprisingly low storage requirements. At run time, we dynamically apply transfer functions to the pdfs using simple and fast convolutions. Whereas standard low-pass filtering and down-sampling incur visible differences between resolution levels, the use of pdfs facilitates consistent results independent of the resolution level used. We describe the efficient out-of-core computation of large-scale sparse pdf volumes, using a novel iterative simplification procedure of a mixture of 4D Gaussians. Finally, our data structure is optimized to facilitate interactive multi-resolution volume rendering on GPUs.

  5. Sparse PDF Volumes for Consistent Multi-Resolution Volume Rendering

    PubMed Central

    Sicat, Ronell; Krüger, Jens; Möller, Torsten; Hadwiger, Markus

    2015-01-01

    This paper presents a new multi-resolution volume representation called sparse pdf volumes, which enables consistent multi-resolution volume rendering based on probability density functions (pdfs) of voxel neighborhoods. These pdfs are defined in the 4D domain jointly comprising the 3D volume and its 1D intensity range. Crucially, the computation of sparse pdf volumes exploits data coherence in 4D, resulting in a sparse representation with surprisingly low storage requirements. At run time, we dynamically apply transfer functions to the pdfs using simple and fast convolutions. Whereas standard low-pass filtering and down-sampling incur visible differences between resolution levels, the use of pdfs facilitates consistent results independent of the resolution level used. We describe the efficient out-of-core computation of large-scale sparse pdf volumes, using a novel iterative simplification procedure of a mixture of 4D Gaussians. Finally, our data structure is optimized to facilitate interactive multi-resolution volume rendering on GPUs. PMID:26146475

  6. The needs of salmon and steelhead in balancing their conservation and use

    USGS Publications Warehouse

    Burger, Carl V.; Knudsen, E. Eric; Steward, Cleveland R.; MacDonald, Donald D.; Williams, Jack E.; Reiser, Dudley W.

    1999-01-01

    Over the past 100 years, Pacific salmon Oncorhynchus spp. and steelhead trout O. mykiss populations in the Pacific Northwest have experienced dramatic declines as a result of human population growth and associated development of the region's natural resources. Any strategy to reverse those declines will depend on achieving consensus among a diverse group of stakeholders willing to (1) restore damaged habitats and watersheds; (2) consider the biological needs of salmonid fishes; and (3) understand, conserve, and manage existing levels of biodiversity within an ecosystem context. The objective of this chapter is to review and summarize the needs of Pacific salmon and steelhead trout from perspectives that promote their sustainability and perpetuity, and, within that context, provide a framework, for the development of a sustainable fisheries strategy. Although volumes of information are available on the life histories and habitat requirements of Pacific salmonids, new concepts have begun to address the importance of habitat complexity, genetic diversity among locally adapted populations, and the need for appropriate units of conservation. Habitat variability and complexity are the templates that produce diverse, locally adapted populations of Pacific salmon and steelhead trout. Molded by conditions in the environments they colonized, salmon and steelhead have unique adaptations they may have taken hundred i?? perhaps thousands i?? of years to evolve. Any strategy to reverse the declines of salmon and steelhead must focus on conserving both current levels of genetic diversity as well as restoration and maintenance of habitats compatible with the specific needs of individual populations. An argument can be made that we know enough already about the needs of our salmon and steelhead to allow us to make appropriate decisions. There is strong need to integrate what we know about local salmonid adaptations and habitat needs with conservation and management strategies that

  7. Impact of Volume Management on Volume Overload and Rehospitalization in CAPD Patients.

    PubMed

    Xu, Yi; Yang, Shen-Min; Wang, Xiao-Hua; Wang, Hai-Fang; Niu, Mei-E; Yang, Yi-Qun; Lu, Guo-Yuan; Pang, Jian-Hong; Wang, Fei; Li, Lin

    2018-05-01

    Heart failure due to volume overload is a major reason for rehospitalization in continuous ambulatory peritoneal dialysis patients. Strict volume control provides better cardiac functions and blood pressure in this population. Volume management, which is a volume control strategy, may decrease volume overload and related complications. Using a quasi-experimental design, 66 continuous ambulatory peritoneal dialysis patients were randomly assigned to the intervention group ( n = 34) and control group ( n = 32). The patients were followed up for 6 months with scheduled clinic and/or telephone visits; the intervention group adopted volume management strategy, while the control group adopted conventional care. Volume overload and cardiac function were compared between the two groups at the baseline and at 6 months. At Month 6, the intervention group resulted in significant improvement in volume overloaded status, cardiac function, and volume-overload-related rehospitalization. Volume management strategy allows for better control of volume overload and is associated with fewer volume-related readmissions.

  8. Antiviral activity of casein and αs2 casein hydrolysates against the infectious haematopoietic necrosis virus, a rhabdovirus from salmonid fish.

    PubMed

    Rodríguez Saint-Jean, S; De las Heras, A; Carrillo, W; Recio, I; Ortiz-Delgado, J B; Ramos, M; Gomez-Ruiz, J A; Sarasquete, C; Pérez-Prieto, S I

    2013-05-01

    Salmonid fish viruses, such as infectious haematopoietic necrosis virus (IHNV), are responsible for serious losses in the rainbow trout and salmon-farming industries, and they have been the subject of intense research in the field of aquaculture. Thus, the aim of this work is to study the antiviral effect of milk-derived proteins as bovine caseins or casein-derived peptides at different stages during the course of IHNV infection. The results indicate that the 3-h fraction of casein and α(S2) -casein hydrolysates reduced the yield of infectious IHNV in a dose-dependent manner and impaired the production of IHNV-specific antigens. Hydrolysates of total casein and α(S2) -casein target the initial and later stages of viral infection, as demonstrated by the reduction in the infective titre observed throughout multiple stages and cycles. In vivo, more than 50% protection was observed in the casein-treated fish, and the kidney sections exhibited none of the histopathological characteristics of IHNV infection. The active fractions from casein were identified, as well as one of the individual IHNV-inhibiting peptides. Further studies will be required to determine which other peptides possess this activity. These findings provide a basis for future investigations on the efficacy of these compounds in treating other viral diseases in farmed fish and to elucidate the underlying molecular mechanisms of action. However, the present results provide convincing evidence in support of a role for several milk casein fractions as suitable candidates to prevent and treat some fish viral infections. © 2012 Blackwell Publishing Ltd.

  9. The Voronoi volume and molecular representation of molar volume: equilibrium simple fluids.

    PubMed

    Hunjan, Jagtar Singh; Eu, Byung Chan

    2010-04-07

    The Voronoi volume of simple fluids was previously made use of in connection with volume transport phenomena in nonequilibrium simple fluids. To investigate volume transport phenomena, it is important to develop a method to compute the Voronoi volume of fluids in nonequilibrium. In this work, as a first step to this goal, we investigate the equilibrium limit of the nonequilibrium Voronoi volume together with its attendant related molar (molal) and specific volumes. It is proved that the equilibrium Voronoi volume is equivalent to the molar (molal) volume. The latter, in turn, is proved equivalent to the specific volume. This chain of equivalences provides an alternative procedure of computing the equilibrium Voronoi volume from the molar volume/specific volume. We also show approximate methods of computing the Voronoi and molar volumes from the information on the pair correlation function. These methods may be employed for their quick estimation, but also provide some aspects of the fluid structure and its relation to the Voronoi volume. The Voronoi volume obtained from computer simulations is fitted to a function of temperature and pressure in the region above the triple point but below the critical point. Since the fitting function is given in terms of reduced variables for the Lennard-Jones (LJ) model and the kindred volumes (i.e., specific and molar volumes) are in essence equivalent to the equation of state, the formula obtained is a reduced equation state for simple fluids obeying the LJ model potential in the range of temperature and pressure examined and hence can be used for other simple fluids.

  10. Using wood composites as a tool for sustainable forestry

    Treesearch

    Jerrold E. Winandy; Robert W. Wellwood; Salim Hiziroglu

    2005-01-01

    This report provides a summary of technical papers presented in Session #90 of the recent IUFRO XXII World Forestry Congress held in Brisbane, Queensland, Australia, August 8–13, 2005. Papers in this report include the oral presentations, poster presentations, and panel discussions exploring and providing technical information on the potential adaptability and...

  11. In Search of the Dimensions of an Incandescent Light Bulb Filament

    ERIC Educational Resources Information Center

    Ladino, Luis A.; Rondón, Hermilda S.

    2018-01-01

    The purpose of this paper is to present and discuss an alternative solution to an experimental problem given to high school students in the XXII Ibero-American Physics Olympiad held by Colombia this year. From the measurements of electric current and potential difference across a small tungsten filament lamp students should find the dimensions of…

  12. Hydrological and thermal effects of hydropeaking on early life stages of salmonids: A modelling approach for implementing mitigation strategies.

    PubMed

    Casas-Mulet, Roser; Saltveit, Svein Jakob; Alfredsen, Knut Tore

    2016-12-15

    Alterations in hydrological and thermal regimes can potentially affect salmonid early life stages development and survival. The dewatering of salmon spawning redds due to hydropeaking can lead to mortality in early life stages, with higher impact on the alevins as they have lower tolerance to dewatering than the eggs. Flow-related mitigation measures can reduce early life stage mortality. We present a set of modelling tools to assess impacts and mitigation options to minimise the risk of mortality in early life stages in hydropeaking rivers. We successfully modelled long-term hydrological and thermal alterations and consequences for development rates. We estimated the risk of early life stages mortality and assessed the cost-effectiveness of implementing three release-related mitigation options (A,B,C). The economic cost of mitigation was low and ranged between 0.7% and 2.6% of the annual hydropower production. Options reducing the flow during spawning (B and C) in addition to only release minimum flows during development (A) were considered more effective for egg and alevin survival. Options B and C were however constraint by water availability in the system for certain years, and therefore only option A was always feasible. The set of modelling tools used in this study were satisfactory and their applications can be useful especially in systems where little field data is available. Targeted measures built on well-informed modelling tools can be tested on their effectiveness to mitigate dewatering effects vs. the hydropower system capacity to release or conserve water for power production. Environmental flow releases targeting specific ecological objectives can provide better cost-effective options than conventional operational rules complying with general legislation. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Development of automatic visceral fat volume calculation software for CT volume data.

    PubMed

    Nemoto, Mitsutaka; Yeernuer, Tusufuhan; Masutani, Yoshitaka; Nomura, Yukihiro; Hanaoka, Shouhei; Miki, Soichiro; Yoshikawa, Takeharu; Hayashi, Naoto; Ohtomo, Kuni

    2014-01-01

    To develop automatic visceral fat volume calculation software for computed tomography (CT) volume data and to evaluate its feasibility. A total of 24 sets of whole-body CT volume data and anthropometric measurements were obtained, with three sets for each of four BMI categories (under 20, 20 to 25, 25 to 30, and over 30) in both sexes. True visceral fat volumes were defined on the basis of manual segmentation of the whole-body CT volume data by an experienced radiologist. Software to automatically calculate visceral fat volumes was developed using a region segmentation technique based on morphological analysis with CT value threshold. Automatically calculated visceral fat volumes were evaluated in terms of the correlation coefficient with the true volumes and the error relative to the true volume. Automatic visceral fat volume calculation results of all 24 data sets were obtained successfully and the average calculation time was 252.7 seconds/case. The correlation coefficients between the true visceral fat volume and the automatically calculated visceral fat volume were over 0.999. The newly developed software is feasible for calculating visceral fat volumes in a reasonable time and was proved to have high accuracy.

  14. Linking spatially distributed biogeochemical data with a two-host life-cycle pathogen:A model of whirling disease dynamics in salmonid fishes in the Intermountain West

    NASA Astrophysics Data System (ADS)

    Fytilis, N.; Lamb, R.; Stevens, L.; Morrissey, L. A.; Kerans, B.; Rizzo, D. M.

    2010-12-01

    Fish diseases are often caused by waterborne parasites, making them ideal systems for modeling the non-linear relationships between biogeochemical features and disease dynamics. Myxobolus cerebralis, the causative agent of whirling disease, has been a major contributor to the loss of wild rainbow trout populations in numerous streams within the Intermountain West (Colorado, Idaho, Montana, Utah, Wyoming). The parasite alternates between an invertebrate and vertebrate host, being transmitted between the sediment feeding worm T.Tubifex and salmonid fishes. A greater understanding of the linkage between biological stream integrity, geomorphic features, water quality parameters and whirling disease risk is needed to improve current management techniques. Biodiversity and abundance of the worm communities are influenced by biogeochemical features and linked to disease severity in fish. We collected and identified ~700 worms from eight sites using molecular genetic probes and a taxonomic key. Additionally, ~1700 worms were identified using only a taxonomic key. Our work examines the links between worm community structure and biogeochemical features. We use a modified Self-Organizing-Map (SOM), which is a non-parametric clustering method based on an artificial neural network (ANN). Clustering methods are particularly attractive for exploratory data analyses because they do not require either the target number of groupings or the data structure be specified at the outset. ANN clustering methods have been shown to be more robust and to account for more data variability than traditional methods when applied to clustering geo-hydrochemical and microbiological datasets. The SOM highlights spatial variation of worm community structure between sites; and is used in tandem with expert knowledge (Lamb and Kerans) of local worm communities and a Madison River, MT physiochemical dataset (GIS-derived layers, water quality parameters). We iteratively clustered the physiochemical data

  15. The Legacy of Manfred Held with Critique

    DTIC Science & Technology

    2011-08-01

    President Executive Vice President and Provost The report entitled “The Legacy of Manfred Held with Critique” contains the results of research...xxii THIS PAGE INTENTIONALLY LEFT BLANK xxiii EXECUTIVE SUMMARY Prof (Dr) Manfred Held...de choc des explosifs solides. Propellants and Explosives, 6, 63-66. [013] Held, M. (1987). Experiments of initiation of covered, but unconfined

  16. A Markov chain analysis of the movements of juvenile salmonids in the forebay of McNary Dam, Washington and Oregon, 2006-09

    USGS Publications Warehouse

    Adams, Noah S.; Hatton, Tyson W.

    2012-01-01

    Passage and survival data for yearling and subyearling Chinook salmon and juvenile steelhead were collected at McNary Dam between 2006 and 2009. These data have provided critical information for resource managers to implement structural and operational changes designed to improve the survival of juvenile salmonids as they migrate past the dam. Much of the information collected at McNary Dam was in the form of three-dimensional tracks of fish movements in the forebay. These data depicted the behavior of multiple species (in three dimensions) during different diel periods, spill conditions, powerhouse operations, and test configurations of the surface bypass structures (temporary spillway weirs; TSWs). One of the challenges in reporting three-dimensional results is presenting the information in a manner that allows interested parties to summarize the behavior of many fish over many different conditions across multiple years. To accomplish this, we investigated the feasibility of using a Markov chain analysis to characterize fish movement patterns in the forebay of McNary Dam. The Markov chain analysis is one way that can be used to summarize numerically the behavior of fish in the forebay. Numerically summarizing the behavior of juvenile salmonids in the forebay of McNary Dam using the Markov chain analysis allowed us to confirm what had been previously summarized using visualization software. For example, proportions of yearling and subyearling Chinook salmon passing the three powerhouse areas was often greater in the southern and middle areas, compared to the northern area. The opposite generally was observed for steelhead. Results of this analysis also allowed us to confirm and quantify the extent of milling behavior that had been observed for steelhead. For fish that were first detected in the powerhouse region, less than 0.10 of the steelhead, on average, passed within each of the powerhouse areas. Instead, steelhead transitioned to adjoining areas in the

  17. Molecular characterization of infectious pancreatic necrosis virus strains isolated from the three types of salmonids farmed in Chile.

    PubMed

    Manríquez, René A; Vera, Tamara; Villalba, Melina V; Mancilla, Alejandra; Vakharia, Vikram N; Yañez, Alejandro J; Cárcamo, Juan G

    2017-01-31

    The infectious pancreatic necrosis virus (IPNV) causes significant economic losses in Chilean salmon farming. For effective sanitary management, the IPNV strains present in Chile need to be fully studied, characterized, and constantly updated at the molecular level. In this study, 36 Chilean IPNV isolates collected over 6 years (2006-2011) from Salmo salar, Oncorhynchus mykiss, and Oncorhynchus kisutch were genotypically characterized. Salmonid samples were obtained from freshwater, estuary, and seawater sources from central, southern, and the extreme-south of Chile (35° to 53°S). Sequence analysis of the VP2 gene classified 10 IPNV isolates as genogroup 1 and 26 as genogroup 5. Analyses indicated a preferential, but not obligate, relationship between genogroup 5 isolates and S. salar infection. Fifteen genogroup 5 and nine genogroup 1 isolates presented VP2 gene residues associated with high virulence (i.e. Thr, Ala, and Thr at positions 217, 221, and 247, respectively). Four genogroup 5 isolates presented an oddly long VP5 deduced amino acid sequence (29.6 kDa). Analysis of the VP2 amino acid motifs associated with clinical and subclinical infections identified the clinical fingerprint in only genogroup 5 isolates; in contrast, the genogroup 1 isolates presented sequences predominantly associated with the subclinical fingerprint. Predictive analysis of VP5 showed an absence of transmembrane domains and plasma membrane tropism signals. WebLogo analysis of the VP5 BH domains revealed high identities with the marine birnavirus Y-6 and Japanese IPNV strain E1-S. Sequence analysis for putative 25 kDa proteins, coded by the ORF between VP2 and VP4, exhibited three putative nuclear localization sequences and signals of mitochondrial tropism in two isolates. This study provides important advances in updating the characterizations of IPNV strains present in Chile. The results from this study will help in identifying epidemiological links and generating specific

  18. Is orbital volume associated with eyeball and visual cortex volume in humans?

    PubMed

    Pearce, Eiluned; Bridge, Holly

    2013-01-01

    In humans orbital volume increases linearly with absolute latitude. Scaling across mammals between visual system components suggests that these larger orbits should translate into larger eyes and visual cortices in high latitude humans. Larger eyes at high latitudes may be required to maintain adequate visual acuity and enhance visual sensitivity under lower light levels. To test the assumption that orbital volume can accurately index eyeball and visual cortex volumes specifically in humans. Structural Magnetic Resonance Imaging (MRI) techniques are employed to measure eye and orbit (n = 88) and brain and visual cortex (n = 99) volumes in living humans. Facial dimensions and foramen magnum area (a proxy for body mass) were also measured. A significant positive linear relationship was found between (i) orbital and eyeball volumes, (ii) eyeball and visual cortex grey matter volumes and (iii) different visual cortical areas, independently of overall brain volume. In humans the components of the visual system scale from orbit to eye to visual cortex volume independently of overall brain size. These findings indicate that orbit volume can index eye and visual cortex volume in humans, suggesting that larger high latitude orbits do translate into larger visual cortices.

  19. Combining Bioenergetic Responses of Fish to Thermal Regimes and Productivity in Reservoirs: Implications for Conservation and Re-Introduction of Anadromous Salmonids

    NASA Astrophysics Data System (ADS)

    Beauchamp, D.

    2014-12-01

    Temperature, food availability, and predation risk form vertical gradients determining growth and survival for fish in lakes and reservoirs. These gradients change on inter-annual, seasonal, and diel temporal scales and are strongly influenced by climatic variability, conflicting water demands and management. Temperatures associated with optimal growth and energy loss vary both among life stages and species of fish, but the quantity and quality of available food resources can significantly alter these thermal responses. Greater understanding of how water management affects the timing, magnitude, and duration of thermal stratification, and how key species and their supporting aquatic resources respond can improve strategies for development and operation of water storage facilities within the context of localized environmental and ecological constraints. An emerging trend for coldwater reservoirs in the Pacific Northwest has been to re-introduce anadromous salmon above historically impassable dams. Thermal regimes and the existing ecological communities in the reservoirs and tributary habitats above these dams will determine the seasonal importance of lotic and lentic habitats for rearing or migration corridors. The feasibility of reservoir rearing and migration can be evaluated by combining mass- and species-specific thermal growth response curves with temporal dynamics in the vertical and longitudinal thermal structure of reservoirs and associated distribution of food resources (primarily zooplankton). The value of reservoirs as rearing habitats or migration corridors could be compared with coincident tributary conditions to predict the likely temporal-spatial distribution of optimal conditions for growth and survival of different species or life stages of salmonids within the watershed and how these conditions might change under different climatic or water management scenarios.

  20. A Markov chain analysis of the movements of juvenile salmonids, including sockeye salmon, in the forebay of McNary Dam, Washington and Oregon, 2006-09

    USGS Publications Warehouse

    Adams, Noah S.; Hatton, Tyson W.

    2012-01-01

    Passage and survival data were collected at McNary Dam between 2006 and 2009. These data have provided critical information for resource managers to implement structural and operational changes designed to improve the survival of juvenile salmonids as they migrate past the dam. Much of the valuable information collected at McNary Dam was in the form of three-dimensional (hereafter referred to as 3-D) tracks of fish movements in the forebay. These data depicted the behavior of multiple species (in three dimensions) during different diel periods, spill conditions, powerhouse operations, and testing of the surface bypass structures (temporary spillway weirs; TSWs). One of the challenges in reporting 3-D results is presenting the information in a manner that allows interested parties to summarize the behavior of many fish over many different conditions across multiple years. To accomplish this, we used a Markov chain analysis to characterize fish movement patterns in the forebay of McNary Dam. The Markov chain analysis allowed us to numerically summarize the behavior of fish in the forebay. This report is the second report published in 2012 that uses this analytical method. The first report included only fish released as part of the annual studies conducted at McNary Dam. This second report includes sockeye salmon that were released as part of studies conducted by the Chelan and Grant County Public Utility Districts at mid-Columbia River dams. The studies conducted in the mid-Columbia used the same transmitters as were used for McNary Dam studies, but transmitter pulse width was different between studies. Additionally, no passive integrated transponder tags were implanted in sockeye salmon. Differences in transmitter pulse width resulted in lower detection probabilities for sockeye salmon at McNary Dam. The absence of passive integrated transponder tags prevented us from determining if fish passed the powerhouse through the juvenile bypass system (JBS) or turbines. To

  1. Is orbital volume associated with eyeball and visual cortex volume in humans?

    PubMed Central

    Pearce, Eiluned; Bridge, Holly

    2013-01-01

    Background In humans orbital volume increases linearly with absolute latitude. Scaling across mammals between visual system components suggests that these larger orbits should translate into larger eyes and visual cortices in high latitude humans. Larger eyes at high latitudes may be required to maintain adequate visual acuity and enhance visual sensitivity under lower light levels. Aim To test the assumption that orbital volume can accurately index eyeball and visual cortex volumes specifically in humans. Subjects & Methods Structural Magnetic Resonance Imaging (MRI) techniques are employed to measure eye and orbit (N=88), and brain and visual cortex (N=99) volumes in living humans. Facial dimensions and foramen magnum area (a proxy for body mass) were also measured. Results A significant positive linear relationship was found between (i) orbital and eyeball volumes, (ii) eyeball and visual cortex grey matter volumes, (iii) different visual cortical areas, independently of overall brain volume. Conclusion In humans the components of the visual system scale from orbit to eye to visual cortex volume independently of overall brain size. These findings indicate that orbit volume can index eye and visual cortex volume in humans, suggesting that larger high latitude orbits do translate into larger visual cortices. PMID:23879766

  2. Evidence for competitive dominance of Pink salmon (Oncorhynchus gorbuscha) over other Salmonids in the North Pacific Ocean

    USGS Publications Warehouse

    Ruggerone, G.T.; Nielsen, J.L.

    2004-01-01

    Relatively little is known about fish species interactions in offshore areas of the world's oceans because adequate experimental controls are typically unavailable in such vast areas. However, pink salmon (Oncorhynchus gorbuscha) are numerous and have an alternating-year pattern of abundance that provides a natural experimental control to test for interspecific competition in the North Pacific Ocean and Bering Sea. Since a number of studies have recently examined pink salmon interactions with other salmon, we reviewed them in an effort to describe patterns of interaction over broad regions of the ocean. Research consistently indicated that pink salmon significantly altered prey abundance of other salmon species (e.g., zooplankton, squid), leading to altered diet, reduced total prey consumption and growth, delayed maturation, and reduced survival, depending on species and locale. Reduced survival was observed in chum salmon (O. keta) and Chinook salmon (O. tshawytscha) originating from Puget Sound and in Bristol Bay sockeye salmon (O. nerka). Growth of pink salmon was not measurably affected by other salmon species, but their growth was sometimes inversely related to their own abundance. In all marine studies, pink salmon affected other species through exploitation of prey resources rather than interference. Interspecific competition was observed in nearshore and offshore waters of the North Pacific Ocean and Bering Sea, and one study documented competition between species originating from different continents. Climate change had variable effects on competition. In the North Pacific Ocean, competition was observed before and after the ocean regime shift in 1977 that significantly altered abundances of many marine species, whereas a study in the Pacific Northwest reported a shift from predation- to competition-based mortality in response to the 1982/1983 El Nino. Key traits of pink salmon that influenced competition with other salmonids included great abundance, high

  3. The North American species of Pholiota

    Treesearch

    Alexander H. Smith; L. R. Hesler

    1968-01-01

    Fries (1821) established Tribe xxii, Pholiota of Agaricus, for many of the species here placed in the genus Pholiota. The history of the genus may be said to start from this publication since it is the official starting date for the nomenclature of agarics. Although the Greek word Pholiota means scale, some glabrous species such as Agaricus caperatus were admitted to...

  4. Correlation of ultrasound estimated placental volume and umbilical cord blood volume in term pregnancy.

    PubMed

    Pannopnut, Papinwit; Kitporntheranunt, Maethaphan; Paritakul, Panwara; Kongsomboon, Kittipong

    2015-01-01

    To investigate the correlation between ultrasound measured placental volume and collected umbilical cord blood (UCB) volume in term pregnancy. An observational cross-sectional study of term singleton pregnant women in the labor ward at Maha Chakri Sirindhorn Medical Center was conducted. Placental thickness, height, and width were measured using two-dimensional (2D) ultrasound and calculated for placental volume using the volumetric mathematic model. After the delivery of the baby, UCB was collected and measured for its volume immediately. Then, birth weight, placental weight, and the actual placental volume were analyzed. The Pearson's correlation was used to determine the correlation between each two variables. A total of 35 pregnant women were eligible for the study. The mean and standard deviation of estimated placental volume and actual placental volume were 534±180 mL and 575±118 mL, respectively. The median UCB volume was 140 mL (range 98-220 mL). The UCB volume did not have a statistically significant correlation with the estimated placental volume (correlation coefficient 0.15; p=0.37). However, the UCB volume was significantly correlated with the actual placental volume (correlation coefficient 0.62; p<0.001) and birth weight (correlation coefficient 0.38; p=0.02). The estimated placental volume by 2D ultrasound was not significantly correlated with the UCB volume. Further studies to establish the correlation between the UCB volume and the estimated placental volume using other types of placental imaging may be needed.

  5. Intracranial Volume and Whole Brain Volume in Infants With Unicoronal Craniosynostosis

    PubMed Central

    Hill, Cheryl A.; Vaddi, S.; Moffitt, Amanda; Kane, A.A.; Marsh, Jeffrey L.; Panchal, Jayesh; Richtsmeier, Joan T.; Aldridge, Kristina

    2011-01-01

    Objective Craniosynostosis has been hypothesized to result in alterations of the brain and cerebral blood flow due to reduced intracranial volume, potentially leading to cognitive deficits. In this study we test the hypothesis that intracranial volume and whole brain volume in infants with unilateral coronal synostosis differs from those in unaffected infants. Design Our study sample consists of magnetic resonance images acquired from 7- to 72-week-old infants with right unilateral coronal synostosis prior to surgery (n = 10) and age-matched unaffected infants (n = 10). We used Analyze 9.0 software to collect three cranial volume measurements. We used nonparametric tests to determine whether the three measures differ between the two groups. Correlations were calculated between age and the three volume measures in each group to determine whether the growth trajectory of the measurements differ between children with right unicoronal synostosis and unaffected infants. Results Our results show that the three volume measurements are not reduced in infants with right unicoronal synostosis relative to unaffected children. Correlation analyses between age and various volume measures show similar correlations in infants with right unicoronal synostosis compared with unaffected children. Conclusions Our results show that the relationship between brain size and intracranial size in infants with right unicoronal synostosis is similar to that in unaffected children, suggesting that reduced intracranial volume is not responsible for alterations of the brain in craniosynostosis. PMID:20815706

  6. An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV

    PubMed Central

    2010-01-01

    Background Due to the limited number of species specific antibodies against fish proteins, differential gene expression analyses are vital for the study of host immune responses. Quantitative real-time reverse transcription PCR (qRT-PCR) is one of the most powerful tools for this purpose. Nevertheless, the accuracy of the method will depend on the careful selection of genes whose expression are stable and can be used as internal controls for a particular experimental setting. Findings The expression stability of five commonly used housekeeping genes [beta-actin (ACTB), elongation factor 1-alpha (EF1A), ubiquitin (UBQ), glyceraldehyd-3-phosphate dehydrogenase (GAPDH) and tubulin alpha (TUBA)] were monitored in salmonid cell lines CHSE-214 and RTS11 after infection with two of the most fastidious fish pathogens, the facultative bacterium Piscirickettsia salmonis and the aquabirnavirus IPNV (Infectious Pancreatic Necrosis Virus). After geNorm analysis, UBQ and EF1A appeared as the most stable, although EF1A was slightly upregulated at late stages of P. salmonis infection in RTS11. ACTB instead, showed a good performance in each case, being always considered within the three most stable genes of the panel. In contrast, infection-dependent differential regulation of GAPDH and TUBA was also demonstrated. Conclusion Based on the data presented here with the cell culture models CHSE-214 and RTS11, we suggest the initial choice of UBQ, ACTB and EF1A as reference genes in qRT-PCR assays for studying the effect of P. salmonis and IPNV on the host immune response. PMID:20398263

  7. Characterization of the live salmonid movement network in Ireland: Implications for disease prevention and control.

    PubMed

    Yatabe, T; More, S J; Geoghegan, F; McManus, C; Hill, A E; Martínez-López, B

    2015-11-01

    Live fish movement is considered as having an important role in the transmission of infectious diseases. For that reason, interventions for cost-effective disease prevention and control rely on a sound understanding of the patterns of live fish movements in a region or country. Here, we characterize the network of live fish movements in the Irish salmonid farming industry during 2013, using social network analysis and spatial epidemiology methods, and identify interventions to limit the risk of disease introduction and spread. In the network there were 62 sites sending and/or receiving fish, with a total of 130 shipments (84 arcs) comprising approx. 17.2 million fish during the year. Atlantic salmon shipments covered longer distances than trout shipments, with some traversing the entire country. The average shipment of Atlantic salmon was 146,186 (SD 194,344) fish, compared to 77,928 (127,009) for trout, however, variability was high. There were 3 periods where shipments peaked (February-April, June-September, and November), which were related to specific stages of fish. The network was disconnected and had two major weak components, the first one with 39 nodes (mostly Atlantic salmon sites), and the second one with 10 nodes (exclusively trout sites). Correlation between in and out-degree at each site and assortativity coefficient were slightly low and non-significant: -0.08 (95% CI: -0.22, 0.06) and -0.13 (95% CI: -0.36, 0.08), respectively, indicating random mixing with regard to node degree. Although competing models also produced a good fit to degree distribution, it is likely that the network possesses both small-world and scale-free topology. This would facilitate the spread and persistence of infection in the salmon production system, but would also facilitate the design of risk-based surveillance strategies by targeting hubs, bridges or cut-points. Using Infomap community detection algorithms, 2 major communities were identified within the giant weak

  8. Variability in triactinomyxon production from Tubifex tubifex populations from the same mitochondrial DNA lineage infected with Myxobolus cerebralis, the causative agent of whirling disease in salmonids

    USGS Publications Warehouse

    Rasmussen, C.; Zickovich, J.; Winton, J.R.; Kerans, B.L.

    2008-01-01

    Myxobolus cerebralis, the causative agent of whirling disease, infects both salmonid fish and an aquatic oligochaete, Tubifex tubifex. Although M. cerebralis has been detected in river drainages throughout the United States, disease severity among wild fish populations has been highly variable. Tubifex tubifex populations have been genetically characterized using sequences from the 16S mitochondrial DNA (mtDNA) gene, the 18S ribosomal RNA gene, the internal transcribed spacer region 1 (ITS1), and randomly amplified polymorphic DNA (RAPD). Our earlier work indicated that large differences in compatibility between the parasite and populations of T. tubifex may play a substantial role in the distribution of whirling disease and resulting mortality in different watersheds. In the present study, we examined 4 laboratory populations of T. tubifex belonging to 16S mtDNA lineage III and 1 population belonging to 16S mtDNA lineage I for triactinomyxon (TAM) production after infection with M. cerebralis myxospores. All 4 16S mtDNA lineage III populations produced TAMs, but statistically significant differences in TAM production were observed. Most individuals in the 16S mtDNA lineage III-infected populations produced TAMs. The 16S mtDNA lineage I population produced few TAMs. Further genetic characterization of the 16S mtDNA lineage III populations with RAPD markers indicated that populations producing similar levels of TAMs had more genetic similarity. ?? American Society of Parasitologists 2008.

  9. Impacts of road deicing salts on the early-life growth and development of a stream salmonid: Salt type matters.

    PubMed

    Hintz, William D; Relyea, Rick A

    2017-04-01

    The use of road deicing salts in regions that experience cold winters is increasing the salinity of freshwater ecosystems, which threatens freshwater resources. Yet, the impacts of environmentally relevant road salt concentrations on freshwater organisms are not well understood, particularly in stream ecosystems where salinization is most severe. We tested the impacts of deicing salts-sodium chloride (NaCl), magnesium chloride (MgCl 2 ), and calcium chloride (CaCl 2 )-on the growth and development of newly hatched rainbow trout (Oncorhynchus mykiss). We exposed rainbow trout to a wide range of environmentally relevant chloride concentrations (25, 230, 860, 1500, and 3000 mg Cl -  L -1 ) over an ecologically relevant time period (25 d). We found that the deicing salts studied had distinct effects. MgCl 2 did not affect rainbow trout growth at any concentration. NaCl had no effects at the lowest three concentrations, but rainbow trout length was reduced by 9% and mass by 27% at 3000 mg Cl -  L -1 . CaCl 2 affected rainbow trout growth at 860 mg Cl -  L -1 (5% reduced length; 16% reduced mass) and these effects became larger at higher concentrations (11% reduced length; 31% reduced mass). None of the deicing salts affected rainbow trout development. At sub-lethal and environmentally relevant concentrations, our results do not support the paradigm that MgCl 2 is the most toxic deicing salt to fish, perhaps due to hydration effects on the Mg 2+ cation. Our results do suggest different pathways for lethal and sub-lethal effects of road salts. Scaled to the population level, the reduced growth caused by NaCl and CaCl 2 at critical early-life stages has the potential to negatively affect salmonid recruitment and population dynamics. Our findings have implications for environmental policy and management strategies that aim to reduce the impacts of salinization on freshwater organisms. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. DEPARTURE OF HIGH-TEMPERATURE IRON LINES FROM THE EQUILIBRIUM STATE IN FLARING SOLAR PLASMAS

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kawate, T.; Keenan, F. P.; Jess, D. B., E-mail: t.kawate@qub.ac.uk

    2016-07-20

    The aim of this study is to clarify if the assumption of ionization equilibrium and a Maxwellian electron energy distribution is valid in flaring solar plasmas. We analyze the 2014 December 20 X1.8 flare, in which the Fe xxi 187 Å, Fe xxii 253 Å, Fe xxiii 263 Å, and Fe xxiv 255 Å emission lines were simultaneously observed by the EUV Imaging Spectrometer on board the Hinode satellite. Intensity ratios among these high-temperature Fe lines are compared and departures from isothermal conditions and ionization equilibrium examined. Temperatures derived from intensity ratios involving these four lines show significant discrepancies atmore » the flare footpoints in the impulsive phase, and at the looptop in the gradual phase. Among these, the temperature derived from the Fe xxii/Fe xxiv intensity ratio is the lowest, which cannot be explained if we assume a Maxwellian electron distribution and ionization equilibrium, even in the case of a multithermal structure. This result suggests that the assumption of ionization equilibrium and/or a Maxwellian electron energy distribution can be violated in evaporating solar plasma around 10 MK.« less

  11. Genetic diversity of Flavobacterium psychrophilum isolates from three Oncorhynchus spp. in the United States, as revealed by multilocus sequence typing

    USDA-ARS?s Scientific Manuscript database

    Flavobacterium psychrophilum is an important pathogen of salmonids worldwide. Multilocus sequence typing (MLST) has identified a recombinogenic population structure from which emerged a few epidemic clonal complexes particularly threatening for salmonid aquaculture. To date, MLST genotypes for this ...

  12. Seasonal habitat use of brook trout and juvenile steelhead in a Lake Ontario tributary

    USGS Publications Warehouse

    Johnson, James H.; Abbett, Ross; Chalupnicki, Marc A.; Verdoliva, Francis

    2016-01-01

    Brook trout (Salvelinus fontinalis) are generally restricted to headwaters in New York tributaries of Lake Ontario. In only a few streams are brook trout abundant in lower stream reaches that are accessible to adult Pacific salmonids migrating from the lake. Consequently, because of the rarity of native brook trout populations in these lower stream reaches it is important to understand how they use stream habitat in sympatry with juvenile Pacific salmonids which are now naturalized in several Lake Ontario tributaries. In this study, we examined the seasonal (spring, summer, and fall) habitat use of brook trout and juvenile steelhead (Oncorhynchus mykiss) in Hart Brook, a tributary of eastern Lake Ontario. We found interspecific, intraspecific, and seasonal variation in habitat use. Subyearling steelhead were associated with faster water velocities than subyearling brook trout and, overall, had the least habitat similarity to the other salmonid groups examined. Overyearling brook trout and yearling steelhead exhibited the greatest degree of habitat selection and habitat selection by all four salmonid groups was greatest in summer. The availability of pool habitat for overyearling salmonids may pose the largest impediment to these species in Hart Brook.

  13. Analyses of potential factors affecting survival of juvenile salmonids volitionally passing through turbines at McNary and John Day Dams, Columbia River

    USGS Publications Warehouse

    Beeman, John; Hansel, Hal; Perry, Russell; Hockersmith, Eric; Sandford, Ben

    2011-01-01

    This report describes analyses of data from radio- or acoustic-tagged juvenile salmonids passing through hydro-dam turbines to determine factors affecting fish survival. The data were collected during a series of studies designed to estimate passage and survival probabilities at McNary (2002-09) and John Day (2002-03) Dams on the Columbia River during controlled experiments of structures or operations at spillways. Relatively few tagged fish passed turbines in any single study, but sample sizes generally were adequate for our analyses when data were combined from studies using common methods over a series of years. We used information-theoretic methods to evaluate biological, operational, and group covariates by creating models fitting linear (all covariates) or curvilinear (operational covariates only) functions to the data. Biological covariates included tag burden, weight, and water temperature; operational covariates included spill percentage, total discharge, hydraulic head, and turbine unit discharge; and group covariates included year, treatment, and photoperiod. Several interactions between the variables also were considered. Support of covariates by the data was assessed by comparing the Akaike Information Criterion of competing models. The analyses were conducted because there was a lack of information about factors affecting survival of fish passing turbines volitionally and the data were available from past studies. The depth of acclimation, tag size relative to fish size (tag burden), turbine unit discharge, and area of entry into the turbine intake have been shown to affect turbine passage survival of juvenile salmonids in other studies. This study indicates that turbine passage survival of the study fish was primarily affected by biological covariates rather than operational covariates. A negative effect of tag burden was strongly supported in data from yearling Chinook salmon at John Day and McNary dams, but not for subyearling Chinook salmon or

  14. Performance Analysis of the Link-16/JTIDS Waveform with Concatenated Coding, Soft Decision Reed-Solomon Decoding, and Noise-Normalization

    DTIC Science & Technology

    2010-09-01

    the time for reviewing instruction, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the...Col Terry Smith for his time spent serving as the second reader of this thesis. xxii THIS PAGE INTENTIONALLY LEFT BLANK 1 I. INTRODUCTION...However, this is the first time that concatenated coding in combination with noise- normalization has been considered. 2 B. THESIS OUTLINE The thesis

  15. Proceedings of the 22nd Texas Symposium On Relativistic Astrophysics At Stanford University

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Chen, P.,; Bloom, Elliott D.,; Madejski, G.,

    2005-09-19

    The XXII Texas Symposium on Relativistic Astrophysics, jointly organized by the Kavli Institute for Particle Astrophysics and Cosmology (KIPAC), the Stanford Linear Accelerator Center, and the Physics Department of Stanford University, was held on December 13-17, 2004. Following the tradition of past Texas Symposia the presentations emphasized recent developments in Cosmology, High Energy Astrophysics and the frontiers between these and Gravitation and Particle Physics.

  16. Investigation into Text Classification With Kernel Based Schemes

    DTIC Science & Technology

    2010-03-01

    Document Matrix TDMs Term-Document Matrices TMG Text to Matrix Generator TN True Negative TP True Positive VSM Vector Space Model xxii THIS PAGE...are represented as a term-document matrix, common evaluation metrics, and the software package Text to Matrix Generator ( TMG ). The classifier...AND METRICS This chapter introduces the indexing capabilities of the Text to Matrix Generator ( TMG ) Toolbox. Specific attention is placed on the

  17. Synthesis and anti-fungal activity of acetylated glycosides of 1,4-naphthoquinone

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Polonik, S.G.; Tolkach, A.M.; Uvarova, N.I.

    1986-12-01

    The authors synthesize a series of glycoside derivatives of 1,4-naphthoquinones (VIII-XXII) and study their anti-fungal activity in a search for more effective preparations for the medical and food industries. The structures of the newly prepared glycosides were verified by IR and /sup 1/H and /sup 13/C NMR spectroscopy. The properties of acetylated 1,4-naphthoquinone glycosides are presented.

  18. Measurements of Dielectronic Recombination Rate Coefficients for He-like and H-like Metal Ions from Tokamak Plasmas

    NASA Astrophysics Data System (ADS)

    Bitter, M.; Hsuan, H.; von Goeler, S.; Hill, K. W.; Grek, B.; Johnson, D. W.; Decaux, V.

    1991-01-01

    The dielectronic recombination rate coefficients associated with the Δn = 1 radiative core transitions have been determined for Ti XXI, Fe XXV, Ni XXVII, and Ti XXII as a function of the electron temperature from the analysis of x-ray satellite spectra emitted from tokamak plasmas. The results obtained are in good agreement with the recent theoretical predictions of Bely-Dubau et al., and Karim and Bhalla.

  19. GEOMORPHOLOGY AND ANTHROPOGENIC INFLUENCES ON FISH AND AMPHIBIANS IN PACIFIC NORTHWEST COASTAL STREAMS

    EPA Science Inventory

    Physical habitat degradation has been implicated as a major contributor to the historic decline of salmonids in Pacific Northwest streams. Native aquatic vertebrate assemblages in the Oregon and Washington Coast Range consist primarily of coldwater salmonids, cottids, and amphib...

  20. Effective climate refugia for salmon in a changing world

    EPA Science Inventory

    Climate change threatens to create fundamental shifts in in the distributions and abundances of endothermic organisms such as cold-water salmon and trout species (salmonids). Recently published projected declines in salmonid distributions under future climates range from modest t...

  1. Climate refugia for salmon in a changing world

    EPA Science Inventory

    Climate change threatens to create fundamental shifts in in the distributions and abundances of endothermic organisms such as cold-water salmon and trout species (salmonids). Recently published projected declines in salmonid distributions under future climates range from modest t...

  2. The volume-outcome relationship and minimum volume standards--empirical evidence for Germany.

    PubMed

    Hentschker, Corinna; Mennicken, Roman

    2015-06-01

    For decades, there is an ongoing discussion about the quality of hospital care leading i.a. to the introduction of minimum volume standards in various countries. In this paper, we analyze the volume-outcome relationship for patients with intact abdominal aortic aneurysm and hip fracture. We define hypothetical minimum volume standards in both conditions and assess consequences for access to hospital services in Germany. The results show clearly that patients treated in hospitals with a higher case volume have on average a significant lower probability of death in both conditions. Furthermore, we show that the hypothetical minimum volume standards do not compromise overall access measured with changes in travel times. Copyright © 2014 John Wiley & Sons, Ltd.

  3. Molecular representation of molar domain (volume), evolution equations, and linear constitutive relations for volume transport.

    PubMed

    Eu, Byung Chan

    2008-09-07

    In the traditional theories of irreversible thermodynamics and fluid mechanics, the specific volume and molar volume have been interchangeably used for pure fluids, but in this work we show that they should be distinguished from each other and given distinctive statistical mechanical representations. In this paper, we present a general formula for the statistical mechanical representation of molecular domain (volume or space) by using the Voronoi volume and its mean value that may be regarded as molar domain (volume) and also the statistical mechanical representation of volume flux. By using their statistical mechanical formulas, the evolution equations of volume transport are derived from the generalized Boltzmann equation of fluids. Approximate solutions of the evolution equations of volume transport provides kinetic theory formulas for the molecular domain, the constitutive equations for molar domain (volume) and volume flux, and the dissipation of energy associated with volume transport. Together with the constitutive equation for the mean velocity of the fluid obtained in a previous paper, the evolution equations for volume transport not only shed a fresh light on, and insight into, irreversible phenomena in fluids but also can be applied to study fluid flow problems in a manner hitherto unavailable in fluid dynamics and irreversible thermodynamics. Their roles in the generalized hydrodynamics will be considered in the sequel.

  4. Triploid atlantic salmon (Salmo salar L.) post-smolts accumulate prevalence more slowly than diploid salmon following bath challenge with salmonid alphavirus subtype 3

    PubMed Central

    Moore, Lindsey J.; Nilsen, Tom Ole; Jarungsriapisit, Jiraporn; Fjelldal, Per Gunnar; Stefansson, Sigurd O.; Taranger, Geir Lasse; Patel, Sonal

    2017-01-01

    Triploid Atlantic salmon (Salmo salar L.) may play an important role in the sustainable expansion of the Norwegian aquaculture industry. Therefore, the susceptibility of triploid salmon to common infections such as salmonid alphavirus (SAV), the causative agent of pancreas disease (PD), requires investigation. In this study, shortly after seawater transfer, diploid and triploid post-smolts were exposed to SAV type 3 (SAV3) using a bath challenge model where the infectious dose was 48 TCID50 ml-1 of tank water. Copy number analysis of SAV3 RNA in heart tissue showed that there was no difference in viral loads between the diploids and triploids. Prevalence reached 100% by the end of the 35-day experimental period in both infected groups. However, prevalence accumulated more slowly in the triploid group reaching 19% and 56% at 14 and 21 days post exposure (dpe) respectively. Whereas prevalence in the diploid group was 82% and 100% at the same time points indicating some differences between diploid and triploid fish. Both heart and pancreas from infected groups at 14 dpe showed typical histopathological changes associated with pancreas disease. Observation of this slower accumulation of prevalence following a natural infection route was possible due to the early sampling points and the exposure to a relatively low dose of virus. The triploid salmon in this study were not more susceptible to SAV3 than diploid salmon indicating that they could be used commercially to reduce the environmental impact of escaped farmed fish interbreeding with wild salmon. This is important information regarding the future use of triploid fish in large scale aquaculture where SAV3 is a financial threat to increased production. PMID:28403165

  5. Triploid atlantic salmon (Salmo salar L.) post-smolts accumulate prevalence more slowly than diploid salmon following bath challenge with salmonid alphavirus subtype 3.

    PubMed

    Moore, Lindsey J; Nilsen, Tom Ole; Jarungsriapisit, Jiraporn; Fjelldal, Per Gunnar; Stefansson, Sigurd O; Taranger, Geir Lasse; Patel, Sonal

    2017-01-01

    Triploid Atlantic salmon (Salmo salar L.) may play an important role in the sustainable expansion of the Norwegian aquaculture industry. Therefore, the susceptibility of triploid salmon to common infections such as salmonid alphavirus (SAV), the causative agent of pancreas disease (PD), requires investigation. In this study, shortly after seawater transfer, diploid and triploid post-smolts were exposed to SAV type 3 (SAV3) using a bath challenge model where the infectious dose was 48 TCID50 ml-1 of tank water. Copy number analysis of SAV3 RNA in heart tissue showed that there was no difference in viral loads between the diploids and triploids. Prevalence reached 100% by the end of the 35-day experimental period in both infected groups. However, prevalence accumulated more slowly in the triploid group reaching 19% and 56% at 14 and 21 days post exposure (dpe) respectively. Whereas prevalence in the diploid group was 82% and 100% at the same time points indicating some differences between diploid and triploid fish. Both heart and pancreas from infected groups at 14 dpe showed typical histopathological changes associated with pancreas disease. Observation of this slower accumulation of prevalence following a natural infection route was possible due to the early sampling points and the exposure to a relatively low dose of virus. The triploid salmon in this study were not more susceptible to SAV3 than diploid salmon indicating that they could be used commercially to reduce the environmental impact of escaped farmed fish interbreeding with wild salmon. This is important information regarding the future use of triploid fish in large scale aquaculture where SAV3 is a financial threat to increased production.

  6. ADULT COHO SALMON AND STEELHEAD USE OF BOULDER WEIRS IN SOUTHWEST OREGON STREAMS

    EPA Science Inventory

    The placement of log and boulder structures in streams is a common and often effective technique for improving juvenile salmonid rearing habitat and increasing fish densities. Less frequently examined has been the use of these structures by adult salmonids. In 2004, spawner densi...

  7. Genomic diversity and evolution of the fish pathogen Flavobacterium psychrophilum

    USDA-ARS?s Scientific Manuscript database

    Flavobacterium psychrophilum, the etiological agent of rainbow trout fry syndrome and bacterial cold-water disease in salmonid fish, is currently one of the main bacterial pathogens hampering the productivity of salmonid farming worldwide. In this study, the genomic diversity of the F. psychrophilum...

  8. Enumeration of Salmonids in the Okanogan Basin Using Underwater Video, Performance Period: October 2005 (Project Inception) - 31 December 2006.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Johnson, Peter N.; Rayton, Michael D.; Nass, Bryan L.

    2007-06-01

    The Confederated Tribes of the Colville Reservation (Colville Tribes) identified the need for collecting baseline census data on the timing and abundance of adult salmonids in the Okanogan River Basin in order to determine basin and tributary-specific spawner distributions, evaluate the status and trends of natural salmonid production in the basin, document local fish populations, and augment existing fishery data. This report documents the design, installation, operation and evaluation of mainstem and tributary video systems in the Okanogan River Basin. The species-specific data collected by these fish enumeration systems are presented along with an evaluation of the operation of amore » facility that provides a count of fish using an automated method. Information collected by the Colville Tribes Fish & Wildlife Department, specifically the Okanogan Basin Monitoring and Evaluation Program (OBMEP), is intended to provide a relative abundance indicator for anadromous fish runs migrating past Zosel Dam and is not intended as an absolute census count. Okanogan Basin Monitoring and Evaluation Program collected fish passage data between October 2005 and December 2006. Video counting stations were deployed and data were collected at two locations in the basin: on the mainstem Okanogan River at Zosel Dam near Oroville, Washington, and on Bonaparte Creek, a tributary to the Okanogan River, in the town of Tonasket, Washington. Counts at Zosel Dam between 10 October 2005 and 28 February 2006 are considered partial, pilot year data as they were obtained from the operation of a single video array on the west bank fishway, and covered only a portion of the steelhead migration. A complete description of the apparatus and methodology can be found in 'Fish Enumeration Using Underwater Video Imagery - Operational Protocol' (Nass 2007). At Zosel Dam, totals of 57 and 481 adult Chinook salmon were observed with the video monitoring system in 2005 and 2006, respectively. Run timing

  9. Healthy People 2010: Conference Edition, Volume I [and] Volume II.

    ERIC Educational Resources Information Center

    Department of Health and Human Services, Washington, DC.

    This document contains the two volumes of the Conference Edition of Healthy People 2010, a comprehensive, nationwide health promotion and disease prevention agenda. The first section of Volume I, "Healthy People 2010: Understanding and Improving Health," includes "Introduction,""Leading Health Indicators," and…

  10. High Performance Schools Best Practices Manual. Volume I: Planning [and] Volume II: Design [and] Volume III: Criteria.

    ERIC Educational Resources Information Center

    Eley, Charles, Ed.

    This three-volume manual, focusing on California's K-12 public schools, presents guidelines for establishing schools that are healthy, comfortable, energy efficient, resource efficient, water efficient, secure, adaptable, and easy to operate and maintain. The first volume describes why high performance schools are important, what components are…

  11. Shop for quality or volume? Volume, quality, and outcomes of coronary artery bypass surgery.

    PubMed

    Auerbach, Andrew D; Hilton, Joan F; Maselli, Judith; Pekow, Penelope S; Rothberg, Michael B; Lindenauer, Peter K

    2009-05-19

    Care from high-volume centers or surgeons has been associated with lower mortality rates in coronary artery bypass surgery, but how volume and quality of care relate to each other is not well understood. To determine how volume and differences in quality of care influence outcomes after coronary artery bypass surgery. Observational cohort. 164 hospitals in the United States. 81,289 patients 18 years or older who had coronary artery bypass grafting from 1 October 2003 to 1 September 2005. Hospital and surgeon case volumes were estimated by using a data set. Quality measures were defined by whether patients received specific medications and by counting the number of measures missed. Hierarchical models were used to estimate effects of volume and quality on death and readmission up to 30 days. After adjustment for clinical factors, lowest surgeon volume and highest hospital volume were associated with higher mortality rates and lower readmission risk, respectively. Patients who did not receive aspirin (odds ratio, 1.89 [95% CI, 1.65 to 2.16) or beta-blockers (odds ratio, 1.29 [CI, 1.12 to 1.49]) had higher odds for death, after adjustment for clinical risk factors and case volume. Adjustment for individual quality measures did not alter associations between volume and readmission or death. However, if no quality measures were missed, mortality rates at the lowest-volume centers (adjusted mortality rate, 1.05% [CI, 0.81% to 1.29%]) and highest-volume centers (adjusted mortality rate, 0.98% [CI, 0.72% to 1.25%]) were similar. Because administrative data were used, the quality measures may not replicate measures collected through chart abstraction. Maximizing adherence to quality measures is associated with improved mortality rates, independent of hospital or surgeon volume. California HealthCare Foundation.

  12. Legal and Institutional Constraints on Aquaculture in Dredged Material Containment Areas

    DTIC Science & Technology

    1993-04-01

    and Wildlife Service La Crosse, WI 54602-0818 48 2A. FIVE DRUGS APPROVED FOR FOOD FISH: 1. Oxytetracycline (feed): Salmonids, Catfish. 21 C.F.R. 558.540...Erythromycin Salmonids Bacterial Kidney U. of Idaho (injection) Disease Oxytetracycline Striped Bass Bacterial Auburn U. Infections Formalin Striped Bass

  13. The Design and Analysis of Salmonid Tagging Studies in the Columbia Basin; Volume XII; A Multinomial Model for Estimating Ocean Survival from Salmonid Coded Wire-Tag Data.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ryding, Kristen E.; Skalski, John R.

    1999-06-01

    The purpose of this report is to illustrate the development of a stochastic model using coded wire-tag (CWT) release and age-at-return data, in order to regress first year ocean survival probabilities against coastal ocean conditions and climate covariates.

  14. High air volume to low liquid volume aerosol collector

    DOEpatents

    Masquelier, Donald A.; Milanovich, Fred P.; Willeke, Klaus

    2003-01-01

    A high air volume to low liquid volume aerosol collector. A high volume flow of aerosol particles is drawn into an annular, centripetal slot in a collector which directs the aerosol flow into a small volume of liquid pool contained is a lower center section of the collector. The annular jet of air impinges into the liquid, imbedding initially airborne particles in the liquid. The liquid in the pool continuously circulates in the lower section of the collector by moving to the center line, then upwardly, and through assistance by a rotating deflector plate passes back into the liquid at the outer area adjacent the impinging air jet which passes upwardly through the liquid pool and through a hollow center of the collector, and is discharged via a side outlet opening. Any liquid droplets escaping with the effluent air are captured by a rotating mist eliminator and moved back toward the liquid pool. The collector includes a sensor assembly for determining, controlling, and maintaining the level of the liquid pool, and includes a lower centrally located valve assembly connected to a liquid reservoir and to an analyzer for analyzing the particles which are impinged into the liquid pool.

  15. NASA Thesaurus. Volume 1: Hierarchical listing. Volume 2: Access vocabulary. Volume 3: Definitions

    NASA Technical Reports Server (NTRS)

    1994-01-01

    There are over 17,500 postable terms and some 4,000 nonpostable terms approved for use in the NASA Scientific and Technical Information Database in the Hierarchical Listing of the NASA Thesaurus. The generic structure is presented for many terms. The broader term and narrower term relationships are shown in an indented fashion that illustrates the generic structure better than the more widely used BT and NT listings. Related terms are generously applied, thus enhancing the usefulness of the Hierarchical Listing. Greater access to the Hierarchical Listing may be achieved with the collateral use of Volume 2 - Access Vocabulary and Volume 3 - Definitions.

  16. Lake Roosevelt Fisheries Evaluation Program, Part C; Lake Roosevelt Pelagic Fish Study: Washington Department of Fish and Wildlife, 1998 Annual Report.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Baldwin, Casey; Polacek, Matt; Bonar, Scott

    2002-11-01

    Pelagic fishes, such as kokanee and rainbow trout, provide an important fishery in Lake Roosevelt; however, spawner returns and creel results have been below management goals in recent years. Our objective was to identify factors that potentially limit pelagic fish production in Lake Roosevelt including entrainment, food limitation, piscivory, and other abiotic factors. We estimated the ratio of total fish entrained through Grand Coulee Dam to the pelagic fish abundance for September and October, 1998. If the majority of these fish were pelagic species, then entrainment averaged 10-13% of pelagic fish abundance each month. This rate of entrainment could imposemore » considerable losses to pelagic fish populations on an annual basis. Therefore, estimates of species composition of entrained fish will be important in upcoming years to estimate the proportion of stocked pelagic fish lost through the dam. Food was not limiting for kokanee or rainbow trout populations since growth rates were high and large zooplankton were present in the reservoir. Estimates of survival for kokanee were low (< 0.01 annual) and unknown for rainbow trout. We estimated that the 1997 standing stock biomass of large (>1.1 mm) Daphnia could have supported 0.08 annual survival by kokanee and rainbow trout before fish consumption would have exceeded available biomass during late winter and early spring. Therefore, if recruitment goals are met in the future there may be a bottleneck in food supply for pelagic planktivores. Walleye and northern pikeminnow were the primary piscivores of salmonids in 1996 and 1997. Predation on salmonid prey was rare for rainbow trout and not detected for burbot or smallmouth bass. Northern pikeminnow had the greatest individual potential as a salmonid predator due to their high consumptive demand; however, their overall impact was limited because of their low relative abundance. We modeled the predation impact of 273,524 walleye in 1996, and 39,075 northern

  17. Research Studies Index. Authors and Subjects. Volume 1 through Volume 43 (1929-1975).

    ERIC Educational Resources Information Center

    Drazan, Joseph, Comp.; Scott, Paula, Comp.

    This volume contains author and subject indexes for volumes 1 through 43 (1929-1975) of "Research Studies," a scholarly, multi-disciplinary quarterly published at Washington State University. Each author index entry includes the title, volume, and inclusive pagination of the article. The subject index is a keyword-out-of-context…

  18. Wood anatomy of the neotropical Sapotaceae. XXII, Pradosia

    Treesearch

    B.F. Kukachka

    1981-01-01

    As constituted here, the genus Pradosia consists of a group of closely related species including the species attributed to Glycoxylon and Neopometia. The members of Pradosia are readily separable anatomically from other American Sapotaceae by the presence of silica in the wood rays and of microcrystals (crystal sand) in the axial parenchyma. Although Podoluma does...

  19. Aperiodic Volume Optics

    NASA Astrophysics Data System (ADS)

    Gerke, Tim D.

    Presented in this thesis is an investigation into aperiodic volume optical devices. The three main topics of research and discussion are the aperiodic volume optical devices that we call computer-generated volume holograms (CGVH), defects within periodic 3D photonic crystals, and non-periodic, but ordered 3D quasicrystals. The first of these devices, CGVHs, are designed and investigated numerically and experimentally. We study the performance of multi-layered amplitude computer-generated volume holograms in terms of efficiency and angular/frequency selectivity. Simulation results show that such aperiodic devices can increase diffraction efficiency relative to periodic amplitude volume holograms while maintaining angular and wavelength selectivity. CGVHs are also designed as voxelated volumes using a new projection optimization algorithm. They are investigated using a volumetric diffraction simulation and a standard 3D beam propagation technique as well as experimentally. Both simulation and experiment verify that the structures function according to their design. These represent the first diffractive structures that have the capacity for generating arbitrary transmission and reflection wave fronts and that provide the ability for multiplexing arbitrary functionality given different illumination conditions. Also investigated and discussed in this thesis are 3D photonic crystals and quasicrystals. We demonstrate that these devices can be fabricated using a femtosecond laser direct writing system that is particularly appropriate for fabrication of such arbitrary 3D structures. We also show that these devices can provide 3D partial bandgaps which could become complete bandgaps if fabricated using high index materials or by coating lower index materials with high index metals. Our fabrication method is particularly suited to the fabrication of engineered defects within the periodic or quasi-periodic systems. We demonstrate the potential for fabricating defects within

  20. Conservation and Management for Fish-eating Birds and Endangered Salmon

    Treesearch

    D. D. Roby; K. Collis; D. E. Lyons

    2005-01-01

    A conflict involving piscivorous birds and salmonids in the Pacific Northwest pits the conservation of protected migratory waterbirds against the restoration of Columbia Basin salmonids (Oncorhynchus spp.) that are listed under the U.S. Endangered Species Act. The Columbia River Avian Predation Project is a cooperative, collaborative research project...

  1. 76 FR 31590 - Endangered and Threatened Species; Take of Anadromous Fish

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-01

    ... proposed research programs are intended to increase knowledge of the species and to help guide management... management efforts, and to determine what salmonid life stages suffer the lowest survival and should be a focus of future management practices. Study 1 is a summer/fall juvenile salmonid population abundance...

  2. Curiosity Speaks Volumes

    NASA Image and Video Library

    2012-08-27

    This chart shows increases in the volume of data coming back from NASA Mars Curiosity over recent sols. New capabilities of the Electra relay-radios on MRO and Curiosity have greatly increased the volume of data the rover is sending back from Mars.

  3. CD volume design and verification

    NASA Technical Reports Server (NTRS)

    Li, Y. P.; Hughes, J. S.

    1993-01-01

    In this paper, we describe a prototype for CD-ROM volume design and verification. This prototype allows users to create their own model of CD volumes by modifying a prototypical model. Rule-based verification of the test volumes can then be performed later on against the volume definition. This working prototype has proven the concept of model-driven rule-based design and verification for large quantity of data. The model defined for the CD-ROM volumes becomes a data model as well as an executable specification.

  4. Effect of nitric oxide, perfluorocarbon, and heliox on minute volume measurement and ventilator volumes delivered.

    PubMed

    Devabhaktuni, V G; Torres, A; Wilson, S; Yeh, M P

    1999-08-01

    To determine the effect of heliox, nitric oxide (NO), and perfluorocarbon on differential pressure pneumotachometer characteristics and to determine the effect of heliox on volumes delivered by the Siemens S900C (S900C), and Servo Ventilator 300 (SV300) ventilators. Prospective, laboratory study. Pulmonary laboratory of a tertiary care, nonprofit children's hospital. SV300, S900C ventilator, differential pressure pneumotachometer. Dual pneumotachometers were connected in series to a 0.5-L calibration syringe and a 1-L anesthesia bag creating a closed system. Calibration of the pneumotachometers was done in room air at ambient temperature with 100 strokes. Accepted accuracy of measured volumes is within 0.5%. Flow-conductance curves were constructed using 100 strokes each for heliox (70:30 mixture), NO, and perfluorocarbon. Expired gases of room air and a 70:30 mixture of heliox from the above ventilators were collected into a nondiffusing gas collection bag, and the volume was measured in a chain-compensated gasometer. Ten sets of 500-mL breaths (20 breaths each set) and 100-mL breaths (40 breaths each set) were collected. The paired Student's t-test was used to detect significant differences in measured volumes, with significance defined as p < .01. Volumes measured with the pneumotachometer using 25 ppm of NO, 50 ppm of NO, and perfluorocarbon were within +0.25%, -0.7%, and +0.4%, respectively (p = .155, p = .001, p = .06). Heliox decreased the conductance of the pneumotachometer, thereby increasing the measured volume by 15% (p < .001). However, heliox did not affect its linearity. Heliox had no affect on volumes delivered by the S900C. However, the SV300 delivered 7.9% less volume of heliox at a set tidal volume of 500 mL and 10.8% less at a set tidal volume of 100 mL. A 70:30 mixture of heliox caused a significantly overestimated gas volume measured and, therefore, an underestimated gas volume delivered by SV300. NO at 25 ppm and perfluorocarbon did not

  5. The Relationship between Volume Conservation and a Volume Algorithm for a Rectangular Parallelepiped.

    ERIC Educational Resources Information Center

    Feghali, Issa Nehme

    This study was designed to investigate the relationship between the level of conservation of displaced volume and the degree to which sixth-grade children learn the volume algorithm of a cuboid, namely, volume equals weight times length times height. The problem chosen is based on an apparent discrepancy between the present school programs and…

  6. Understanding the heterogeneity in volume overload and fluid distribution in decompensated heart failure is key to optimal volume management: role for blood volume quantitation.

    PubMed

    Miller, Wayne L; Mullan, Brian P

    2014-06-01

    This study sought to quantitate total blood volume (TBV) in patients hospitalized for decompensated chronic heart failure (DCHF) and to determine the extent of volume overload, and the magnitude and distribution of blood volume and body water changes following diuretic therapy. The accurate assessment and management of volume overload in patients with DCHF remains problematic. TBV was measured by a radiolabeled-albumin dilution technique with intravascular volume, pre-to-post-diuretic therapy, evaluated at hospital admission and at discharge. Change in body weight in relation to quantitated TBV was used to determine interstitial volume contribution to total fluid loss. Twenty-six patients were prospectively evaluated. Two patients had normal TBV at admission. Twenty-four patients were hypervolemic with TBV (7.4 ± 1.6 liters) increased by +39 ± 22% (range, +9.5% to +107%) above the expected normal volume. With diuresis, TBV decreased marginally (+30 ± 16%). Body weight declined by 6.9 ± 5.2 kg, and fluid intake/fluid output was a net negative 8.4 ± 5.2 liters. Interstitial compartment fluid loss was calculated at 6.2 ± 4.0 liters, accounting for 85 ± 15% of the total fluid reduction. TBV analysis demonstrated a wide range in the extent of intravascular overload. Dismissal measurements revealed marginally reduced intravascular volume post-diuretic therapy despite large reductions in body weight. Mobilization of interstitial fluid to the intravascular compartment with diuresis accounted for this disparity. Intravascular volume, however, remained increased at dismissal. The extent, composition, and distribution of volume overload are highly variable in DCHF, and this variability needs to be taken into account in the approach to individualized therapy. TBV quantitation, particularly serial measurements, can facilitate informed volume management with respect to a goal of treating to euvolemia. Copyright © 2014 American College of Cardiology Foundation. Published

  7. Evaluation of juvenile salmonid behavior near a prototype weir box at Cowlitz Falls Dam, Washington, 2013

    USGS Publications Warehouse

    Kock, Tobias J.; Liedtke, Theresa L.; Ekstrom, Brian K.; Tomka, Ryan G.; Rondorf, Dennis W.

    2014-01-01

    Collection of juvenile salmonids at Cowlitz Falls Dam is a critical part of the effort to restore salmon in the upper Cowlitz River because the majority of fish that are not collected at the dam pass downstream and enter a large reservoir where they become landlocked and lost to the anadromous fish population. However, the juvenile fish collection system at Cowlitz Falls Dam has failed to achieve annual collection goals since it first began operating in 1996. Since that time, numerous modifications to the fish collection system have been made and several prototype collection structures have been developed and tested, but these efforts have not substantially increased juvenile fish collection. Studies have shown that juvenile steelhead (Oncorhynchus mykiss), coho salmon (Oncorhynchus kisutch), and Chinook salmon (Oncorhynchus tshawytscha) tend to locate the collection entrances effectively, but many of these fish are not collected and eventually pass the dam through turbines or spillways. Tacoma Power developed a prototype weir box in 2009 to increase capture rates of juvenile salmonids at the collection entrances, and this device proved to be successful at retaining those fish that entered the weir. However, because of safety concerns at the dam, the weir box could not be deployed near a spillway gate where the prototype was tested, so the device was altered and re-deployed at a different location, where it was evaluated during 2013. The U.S. Geological Survey conducted an evaluation using radiotelemetry to monitor fish behavior near the weir box and collection flumes. The evaluation was conducted during April–June 2013. Juvenile steelhead and coho salmon (45 per species) were tagged with a radio transmitter and passive integrated transponder (PIT) tag, and released upstream of the dam. All tagged fish moved downstream and entered the forebay of Cowlitz Falls Dam. Median travel times from the release site to the forebay were 0.8 d for steelhead and 1.2 d for coho

  8. Evaluation of a stream channel-type system for southeast Alaska.

    Treesearch

    M.D. Bryant; P.E. Porter; S.J. Paustian

    1991-01-01

    Nine channel types within a hierarchical channel-type classification system (CTCS) were surveyed to determine relations between salmonid densities and species distribution, and channel type. Two other habitat classification systems and the amount of large woody debris also were compared to species distribution and salmonid densities, and to stream channel types....

  9. An Analysis of the Feedback Provided from the Marine Corps Combat Readiness Evaluation System.

    DTIC Science & Technology

    1983-12-01

    view of management control as that adapted by researchers in cybernetics, account - S -ing, and management information systems--those that concentrate...Conscious Style--concerned with costs but not with meeting a budget. (4) Non- Accounting Style--not concerned with meeting a budget or with costs . (Ref. 21...34 California Manacement Review, Vol. XXII, No. 2, Winter 1979, p. 50-59. 44. Horngren , Charles T. Ph.D., CPA, Introduction to Manage- [. . -ment Accounting

  10. Operational Artillery in the Korean War

    DTIC Science & Technology

    2013-05-23

    employ artillery in a war of annihilation requires adherence to specific principles to maximize the effectiveness of combat power at the right time and...Policy (Bloomington: Indiana University Press, 1977), xxii. 24 “The Korean War rescued NSC-68 from oblivion and made it the foundation of American...multiple firing units at the right time and place with the purpose of supporting the decisive operations of maneuver. Fire planning in the Korean War

  11. Power Beaming, Orbital Debris Removal, and Other Space Applications of a Ground Based Free Electron Laser

    DTIC Science & Technology

    2010-03-01

    mask of strength, his character, fortitude, and xxii devotion to our family helped to keep me on my feet. What I say with words, he says through...superfluid) and an extremely large heat capacity. This large heat capacity is what makes He II an ideal refrigerant for high power and high frequency...limited tools, ancient astronomers accomplished many insightful discoveries regarding the motion of celestial bodies, but prior to the 1600s, most of

  12. An Experimental Study of the Driving Mechanism and Control of the Unsteady Shock Induced Turbulent Separation in a Mach 5 Compression Corner Flow.

    DTIC Science & Technology

    1992-01-01

    vili Table of Contents Section Page List of Tables xiii List of Figures xiv Nomenclature xxii 1 Introduction 1 1.1 Motivation 1 1.2 Objective of...Introduction 1.1 Motivation The mid-1980’s saw a renewed interest in hypersonic flight. Motivated by the achievements of the American Space Transportation... measuments made downstrem of the compreson corner. 4.2.2 VITA Technique Another approach to event detection was applied to the fluctuating pitot

  13. Fisheries and aquatic resources of Prairie Creek, Redwood National Park

    USGS Publications Warehouse

    Wilzbach, Peggy; Ozaki, Vicki

    2017-01-01

    This report synthesizes information on the status of fisheries and aquatic resources in the Prairie Creek sub-basin of Redwood Creek in Humboldt County in northern California, founded on a bibliographic search we conducted of historic and current datasets, unpublished reports, theses, and publications. The compiled Prairie Creek Fisheries Bibliography is available at https://irma.nps.gov/DataStore/. This report describes life histories and population status of the salmonid fishes, and species occurrence of non-salmonid fishes, amphibians, macroinvertebrates, and common benthic algae in Prairie Creek. We assessed habitat conditions that may limit salmonid production in relation to recovery targets established by the National Marine Fisheries Service and the State of California. Although salmon abundance has decreased from historic levels, production of juvenile salmonids in Prairie Creek is relatively stable and robust in comparison with the rest of the Redwood Creek Basin. Carrying capacity likely differs between the undisturbed upper reaches of Prairie Creek and reaches in the lower creek, the latter of which are affected by legacy impacts from timber and agricultural activities. Increased sediment supply and lack of channel structure and floodplain connection in lower Prairie Creek appear to be the greatest stressors to salmonid production. Existing datasets on aquatic resources and environmental variables are listed, and subject areas where few data are available are identified.

  14. River Food Web Response to Large-Scale Riparian Zone Manipulations

    PubMed Central

    Wootton, J. Timothy

    2012-01-01

    Conservation programs often focus on select species, leading to management plans based on the autecology of the focal species, but multiple ecosystem components can be affected both by the environmental factors impacting, and the management targeting, focal species. These broader effects can have indirect impacts on target species through the web of interactions within ecosystems. For example, human activity can strongly alter riparian vegetation, potentially impacting both economically-important salmonids and their associated river food web. In an Olympic Peninsula river, Washington state, USA, replicated large-scale riparian vegetation manipulations implemented with the long-term (>40 yr) goal of improving salmon habitat did not affect water temperature, nutrient limitation or habitat characteristics, but reduced canopy cover, causing reduced energy input via leaf litter, increased incident solar radiation (UV and PAR) and increased algal production compared to controls. In response, benthic algae, most insect taxa, and juvenile salmonids increased in manipulated areas. Stable isotope analysis revealed a predominant contribution of algal-derived energy to salmonid diets in manipulated reaches. The experiment demonstrates that riparian management targeting salmonids strongly affects river food webs via changes in the energy base, illustrates how species-based management strategies can have unanticipated indirect effects on the target species via the associated food web, and supports ecosystem-based management approaches for restoring depleted salmonid stocks. PMID:23284786

  15. Cell volume regulation and apoptotic volume decrease in rat distal colon superficial enterocytes.

    PubMed

    Antico, Stefania; Lionetto, Maria Giulia; Giordano, Maria Elena; Caricato, Roberto; Schettino, Trifone

    2013-01-01

    The colon epithelium is physiologically exposed to osmotic stress, and the activation of cell volume regulation mechanisms is essential in colonocyte physiology. Moreover, colon is characterized by a high apoptotic rate of mature cells balancing the high division rate of stem cells. The aim of the present work was to investigate the main cell volume regulation mechanisms in rat colon surface colonocytes and their role in apoptosis. Cell volume changes were measured by light microscopy and video imaging on colon explants; apoptosis sign appearance was monitored by confocal microscopy on annexin V/propidium iodide labeled explants. Superficial colonocytes showed a dynamic regulation of their cell volume during anisosmotic conditions with a Regulatory Volume Increase (RVI) response following hypertonic shrinkage and Regulatory Volume Decrease (RVD) response following hypotonic swelling. RVI was completely inhibited by bumetanide, while RVD was completely abolished by high K(+) or iberiotoxin treatment and by extracellular Ca(2+) removal. DIDS incubation was also able to affect the RVD response. When colon explants were exposed to H2O2 as apoptotic inducer, colonocytes underwent an isotonic volume decrease ascribable to Apoptotic Volume Decrease (AVD) within about four hours of exposure. AVD was shown to precede annexin V positivity. It was also inhibited by high K(+) or iberiotoxin treatment. Interestingly, treatment with iberiotoxin significantly inhibited apoptosis progression. In rat superficial colonocytes K(+) efflux through high conductance Ca(2+)-activated K(+) channels (BK channels) was demonstrated to be the main mechanism of RVD and to plays also a crucial role in the AVD process and in the progression of apoptosis. © 2013 S. Karger AG, Basel.

  16. Variable volume combustor

    DOEpatents

    Ostebee, Heath Michael; Ziminsky, Willy Steve; Johnson, Thomas Edward; Keener, Christopher Paul

    2017-01-17

    The present application provides a variable volume combustor for use with a gas turbine engine. The variable volume combustor may include a liner, a number of micro-mixer fuel nozzles positioned within the liner, and a linear actuator so as to maneuver the micro-mixer fuel nozzles axially along the liner.

  17. Stroke volume variation as a guide for fluid resuscitation in patients undergoing large-volume liposuction.

    PubMed

    Jain, Anil Kumar; Khan, Asma M

    2012-09-01

    : The potential for fluid overload in large-volume liposuction is a source of serious concern. Fluid management in these patients is controversial and governed by various formulas that have been advanced by many authors. Basically, it is the ratio of what goes into the patient and what comes out. Central venous pressure has been used to monitor fluid therapy. Dynamic parameters, such as stroke volume and pulse pressure variation, are better predictors of volume responsiveness and are superior to static indicators, such as central venous pressure and pulmonary capillary wedge pressure. Stroke volume variation was used in this study to guide fluid resuscitation and compared with one guided by an intraoperative fluid ratio of 1.2 (i.e., Rohrich formula). : Stroke volume variation was used as a guide for intraoperative fluid administration in 15 patients subjected to large-volume liposuction. In another 15 patients, fluid resuscitation was guided by an intraoperative fluid ratio of 1.2. The amounts of intravenous fluid administered in the groups were compared. : The mean amount of fluid infused was 561 ± 181 ml in the stroke volume variation group and 2383 ± 1208 ml in the intraoperative fluid ratio group. The intraoperative fluid ratio when calculated for the stroke volume variation group was 0.936 ± 0.084. All patients maintained hemodynamic parameters (heart rate and systolic, diastolic, and mean blood pressure). Renal and metabolic indices remained within normal limits. : Stroke volume variation-guided fluid application could result in an appropriate amount of intravenous fluid use in patients undergoing large-volume liposuction. : Therapeutic, II.

  18. Volume quantification by contrast-enhanced ultrasound: an in-vitro comparison with true volumes and thermodilution.

    PubMed

    Herold, Ingeborg H F; Russo, Gianna; Mischi, Massimo; Houthuizen, Patrick; Saidov, Tamerlan; van het Veer, Marcel; van Assen, Hans C; Korsten, Hendrikus H M

    2013-10-17

    Contrast-enhanced ultrasound (CEUS) has recently been proposed as a minimally- invasive, alternative method for blood volume measurement. This study aims at comparing the accuracy of CEUS and the classical thermodilution techniques for volume assessment in an in-vitro set-up. The in-vitro set-up consisted of a variable network between an inflow and outflow tube and a roller pump. The inflow and outflow tubes were insonified with an ultrasound array transducer and a thermistor was placed in each tube. Indicator dilution curves were made by injecting indicator which consisted of an ultrasound-contrast-agent diluted in ice-cold saline. Both acoustic intensity- and thermo-dilution curves were used to calculate the indicator mean transit time between the inflow and outflow tube. The volumes were derived by multiplying the estimated mean transit time by the flow rate. We compared the volumes measured by CEUS with the true volumes of the variable network and those measured by thermodilution by Bland-Altman and intraclass-correlation analysis. The measurements by CEUS and thermodilution showed a very strong correlation (rs = 0.94) with a modest volume underestimation by CEUS of -40 ± 28 mL and an overestimation of 84 ± 62 mL by thermodilution compared with the true volumes. Both CEUS and thermodilution showed a high statistically significant correlation with the true volume (rs = 0.97 (95% CI, 0.95 - 0.98; P<0.0001) and rs = 0.96 (95% CI, 0.94 - 0.98; P<0.0001, respectively). CEUS volume estimation provides a strong correlation with both the true volumes in-vitro and volume estimation by thermodilution. It may therefore represent an interesting alternative to the standard, invasive thermodilution technique.

  19. Direct volume estimation without segmentation

    NASA Astrophysics Data System (ADS)

    Zhen, X.; Wang, Z.; Islam, A.; Bhaduri, M.; Chan, I.; Li, S.

    2015-03-01

    Volume estimation plays an important role in clinical diagnosis. For example, cardiac ventricular volumes including left ventricle (LV) and right ventricle (RV) are important clinical indicators of cardiac functions. Accurate and automatic estimation of the ventricular volumes is essential to the assessment of cardiac functions and diagnosis of heart diseases. Conventional methods are dependent on an intermediate segmentation step which is obtained either manually or automatically. However, manual segmentation is extremely time-consuming, subjective and highly non-reproducible; automatic segmentation is still challenging, computationally expensive, and completely unsolved for the RV. Towards accurate and efficient direct volume estimation, our group has been researching on learning based methods without segmentation by leveraging state-of-the-art machine learning techniques. Our direct estimation methods remove the accessional step of segmentation and can naturally deal with various volume estimation tasks. Moreover, they are extremely flexible to be used for volume estimation of either joint bi-ventricles (LV and RV) or individual LV/RV. We comparatively study the performance of direct methods on cardiac ventricular volume estimation by comparing with segmentation based methods. Experimental results show that direct estimation methods provide more accurate estimation of cardiac ventricular volumes than segmentation based methods. This indicates that direct estimation methods not only provide a convenient and mature clinical tool for cardiac volume estimation but also enables diagnosis of cardiac diseases to be conducted in a more efficient and reliable way.

  20. Aircraft Electrical Systems Specialist (AFSC 42350), Volumes 1-3, and Change Supplement, Volume 3.

    ERIC Educational Resources Information Center

    Savage, Leslie R.

    This three-volume student text is designed for use by Air Force personnel enrolled in a self-study extension course for aircraft electrical systems specialists. Covered in the individual volumes are career field fundamentals, electrical systems and test equipment, and aircraft control and warning systems. Each volume in the set contains a series…

  1. Mismatching between nest volume and clutch volume reduces egg survival and fledgling success in black-tailed gulls

    PubMed Central

    Yoo, Jeong-Chil

    2016-01-01

    Abstract A longstanding suggestion posits that parents prefer to match nest volume and clutch size (clutch volume), but few studies have tested this in colonial seabirds that nest in the open. Here, we demonstrate the effects of nest–clutch volume matching on egg survival, hatching, and fledgling success in black-tailed gulls Larus crassirostris on Hongdo Island, Korea. We show that the volume mismatch, defined as the difference between nest volume and total egg volume (the sum of all eggs’ volume in the clutch), was positively related to egg and chick mortality caused by predation, but was not significantly related to hatching success incurred by insulation during the incubation period. Although nest volume was negatively related to laying date, we found that the mismatch was positively related to laying date. Our results support the claim that well-matched nest–clutch volume may contribute to survival of eggs and chicks, and ultimately breeding success. PMID:29491934

  2. Calculus Students' Understanding of Volume

    ERIC Educational Resources Information Center

    Dorko, Allison; Speer, Natasha M.

    2013-01-01

    Researchers have documented difficulties that elementary school students have in understanding volume. Despite its importance in higher mathematics, we know little about college students' understanding of volume. This study investigated calculus students' understanding of volume. Clinical interview transcripts and written responses to volume…

  3. Modeling Approach for Estimating Co-Produced Water Volumes and Saltwater Disposal Volumes in Oklahoma

    NASA Astrophysics Data System (ADS)

    Murray, K. E.

    2016-12-01

    Management of produced fluids has become an important issue in Oklahoma because large volumes of saltwater are co-produced with oil and gas, and disposed into saltwater disposal wells at high rates. Petroleum production increased from 2009-2015, especially in central and north-central Oklahoma where the Mississippian and Hunton zones were redeveloped using horizontal wells and dewatering techniques that have led to a disproportional increase in produced water volumes. Improved management of co-produced water, including desalination for beneficial reuse and decreased saltwater disposal volumes, is only possible if spatial and temporal trends can be defined and related to the producing zones. It is challenging to quantify the volumes of co-produced water by region or production zone because co-produced water volumes are generally not reported. Therefore, the goal of this research is to estimate co-produced water volumes for 2008-present with an approach that can be replicated as petroleum production shifts to other regions. Oil and gas production rates from subsurface zones were multiplied by ratios of H2O:oil and H2O:gas for the respective zones. Initial H2O:oil and H2O:gas ratios were adjusted/calibrated, by zone, to maximize correlation of county-scale produced H2O estimates versus saltwater disposal volumes from 2013-2015. These calibrated ratios were then used to compute saltwater disposal volumes from 2008-2012 because of apparent data gaps in reported saltwater disposal volumes during that timeframe. This research can be used to identify regions that have the greatest need for produced water treatment systems. The next step in management of produced fluids is to explore optimal energy-efficient strategies that reduce deleterious effects.

  4. Emergence and maintenance of infectious salmon anaemia virus (ISAV) in Europe: a new hypothesis.

    PubMed

    Nylund, A; Devold, M; Plarre, H; Isdal, E; Aarseth, M

    2003-08-15

    The present study describes the use of molecular methods in studying infectious salmon anaemia virus (ISAV), an important pathogen of farmed salmon in Norway, Scotland, the Faeroe Islands, Canada, USA and Chile. The nucleotide sequences of the haemagglutinin gene (HA) from 70 ISAV isolates have been analysed for phylogenetic relationship and the average mutation rate of nucleotide substitutions calculated. The isolates constitute 2 major groups, 1 European and 1 North American group. The isolate from Chile is closely related to the North American isolates. The European isolates can be further divided into 3 separate groups reflecting geographical distribution, time of collection, and transmission connected with farming activity. Based on existing information about infectious salmon anaemia (ISA) and new information emerging from the present study, it is hypothesised that: (1) ISAV is maintained in wild populations of trout and salmon in Europe; (2) it is transmitted between wild hosts mainly during their freshwater spawning phase in rivers; (3) wild salmonids, mainly trout, possibly carry benign wild-type ISAV isolates; (4) a change (mutation) in virulence probably results from deletions of amino acid segments from the highly polymorphic region (HPR) of benign wild-type isolates; (5) ISA emerges in farmed Atlantic salmon when mutated isolates are transmitted from wild salmonids or, following mutation of benign isolates, in farmed salmon after transmission from wild salmonids; (6) farming activity is an important factor in transmission of ISAV between farming sites in addition to transmission of ISAV from wild salmonids to farmed salmon; (7) transmission of ISAV from farmed to wild salmonids probably occurs less frequently than transmission from wild to farmed fish due to lower frequency of susceptible wild individuals; (8) the frequency of new outbreaks of ISA in farmed salmon probably reflects natural variation in the prevalence of ISAV in wild populations of

  5. Effects of colony relocation on diet and productivity of Caspian terns

    USGS Publications Warehouse

    Roby, D.D.; Collis, K.; Lyons, Donald E.; Craig, D.P.; Adkins, J.Y.; Myers, A.M.; Suryan, R.M.

    2002-01-01

    We investigated the efficacy of management to reduce the impact of Caspian tern (Sterna caspia) predation on survival of juvenile salmonids (Oncorhynchus spp.) in the Columbia River estuary. Resource managers sought to relocate approximately 9,000 pairs of terns nesting on Rice Island (river km 34) to East Sand Island (river km 8), where terns were expected to prey on fewer juvenile salmonids. Efforts to attract terns to nest on East Sand Island included creation of nesting habitat, use of social attraction techniques, and predator control, with concurrent efforts to discourage terns from nesting on Rice Island. This approach was successful in completely relocating the tern colony from Rice Island to East Sand Island by the third breeding season. Juvenile salmonids decreased and marine forage fishes (i.e., herring, sardine, anchovy, smelt, surfperch, Pacific sand lance) increased in the diet of Caspian terns nesting on East Sand Island, compared with terns nesting on Rice Island. During 1999 and 2000, the diet of terns nesting on Rice Island consisted of 77% and 90% juvenile salmonids, respectively, while during 1999, 2000, and 2001, the diet of terns nesting on East Sand Island consisted of 46%, 47%, and 33% juvenile salmonids, respectively. Nesting success of Caspian terns was consistently and substantially higher on East Sand Island than on Rice Island. These results indicate that relocating the Caspian tern colony was an effective management action for reducing predation on juvenile salmonids without harm to the population of breeding terns, at least in the short term. The success of this management approach largely was a consequence of the nesting and foraging ecology of Caspian terns: the species shifts breeding colony sites frequently in response to changing habitats, and the species is a generalist forager, preying on the most available forage fish near the colony.

  6. Stream channels: The link between forests and fishes

    Treesearch

    Kathleen Sullivan; Thomas E. Lisle; C. Andrew Dolloff; Gordon E. Grant; Leslie M. Reid

    1987-01-01

    Abstract - The hydraulic characteristics of flow through channels are an important component of fish habitat. Salmonids have evolved in stream systems in which water velocity and flow depth vary spatially within the watershed and temporally on a daily, seasonal, and annual basis. Flow requirements vary during different phases of the freshwater life cycle of salmonids...

  7. Identifying when tagged fishes have been consumed by piscivorous predators: application of multivariate mixture models to movement parameters of telemetered fishes

    USGS Publications Warehouse

    Romine, Jason G.; Perry, Russell W.; Johnston, Samuel V.; Fitzer, Christopher W.; Pagliughi, Stephen W.; Blake, Aaron R.

    2013-01-01

    Mixture models proved valuable as a means to differentiate between salmonid smolts and predators that consumed salmonid smolts. However, successful application of this method requires that telemetered fishes and their predators exhibit measurable differences in movement behavior. Our approach is flexible, allows inclusion of multiple track statistics and improves upon rule-based manual classification methods.

  8. Neural mechanisms of volume regulation.

    PubMed

    DiBona, G F

    1983-05-01

    Under steady-state conditions, urinary sodium excretion matches dietary sodium intake. Because extracellular fluid osmolality is tightly regulated, the quantity of sodium in the extracellular fluid determines the volume of this compartment. The left atrial volume receptor mechanism is an example of a neural mechanism of volume regulation. The left atrial mechanoreceptor, which functions as a sensor in the low-pressure vascular system, has a well-defined compliance relating intravascular volume to filling pressure and responds to changes in wall tension by discharging into afferent vagal fibers. These fibers have appropriate central nervous system representation whose related efferent neurohumoral mechanisms regulate thirst, renal excretion of water and sodium, and the redistribution of the extracellular fluid volume.

  9. Whirling disease: host specificity and interaction between the actinosporean stage of Myxobolus cerebralis and rainbow trout Oncorhynchus mykiss.

    PubMed

    el-Matbouli, M; Hoffmann, R W; Schoel, H; McDowell, T S; Hedrick, R P

    1999-01-07

    Scanning electron microscopic studies were conducted on rainbow trout Oncorhynchus mykiss in the first 60 min after their exposure to the triactinomyxon spores of Myxobolus cerebralis. The results demonstrated that as early as 1 min post exposure the whole process, from the attachment of the triactinomyxon spores to the complete penetration of their sporoplasm germs, had occurred. The triactinomyxon spores sought out the secretory openings of mucous cells of the epidermis, the respiratory epithelium and the buccal cavity of trout and used them as portals of entry. Exposure experiments of the triactinomyxon spores of M. cerebralis to non-salmonid fish, such as goldfish Carassius auratus, carp Cyprinus carpio, nose Chondrostoma nasus, medaka Oryzias latipes, guppy Poecilia reticulata and also the amphibian tadpole Rana pipiens as well as to rainbow trout fry indicated a specificity for salmonids. Attempts to activate the triactinomyxon spores by exposure to mucus prepared from cyprinid and salmonid fish showed no significant differences from those conducted in tap water. The results suggest that the simultaneous presence of both mechano- and chemotactic stimuli was required for finding the salmonid fish host.

  10. Diel resource partitioning among juvenile Atlantic Salmon, Brown Trout, and Rainbow Trout during summer

    USGS Publications Warehouse

    Johnson, James H.; McKenna, James E.

    2015-01-01

    Interspecific partitioning of food and habitat resources has been widely studied in stream salmonids. Most studies have examined resource partitioning between two native species or between a native species and one that has been introduced. In this study we examine the diel feeding ecology and habitat use of three species of juvenile salmonids (i.e., Atlantic Salmon Salmo salar, Brown Trout Salmo trutta, and Rainbow Trout Oncorhynchus mykiss) in a tributary of Skaneateles Lake, New York. Subyearling Brown Trout and Rainbow Trout fed more heavily from the drift than the benthos, whereas subyearling Atlantic Salmon fed more from the benthos than either species of trout. Feeding activity of Atlantic Salmon and Rainbow Trout was similar, with both species increasing feeding at dusk, whereas Brown Trout had no discernable feeding peak or trough. Habitat availability was important in determining site-specific habitat use by juvenile salmonids. Habitat selection was greater during the day than at night. The intrastream, diel, intraspecific, and interspecific variation we observed in salmonid habitat use in Grout Brook illustrates the difficulty of acquiring habitat use information for widespread management applications.

  11. Diurnal stream habitat use of juvenile Atlantic salmon, brown trout and rainbow trout in winter

    USGS Publications Warehouse

    Johnson, J. H.; Douglass, K.A.

    2009-01-01

    The diurnal winter habitat of three species of juvenile salmonids was examined in a tributary of Skaneateles Lake, NY to compare habitat differences among species and to determine if species/age classes were selecting specific habitats. A total of 792 observations were made on the depth, velocity, substrate and cover (amount and type) used by sympatric subyearling Atlantic salmon, subyearling brown trout and subyearling and yearling rainbow trout. Subyearling Atlantic salmon occurred in shallower areas with faster velocities and less cover than the other salmonid groups. Subyearling salmon was also the only group associated with substrate of a size larger than the average size substrate in the study reach during both winters. Subyearling brown trout exhibited a preference for vegetative cover. Compared with available habitat, yearling rainbow trout were the most selective in their habitat use. All salmonid groups were associated with more substrate cover in 2002 under high flow conditions. Differences in the winter habitat use of these salmonid groups have important management implications in terms of both habitat protection and habitat enhancement.

  12. Perennial snow and ice volumes on Iliamna Volcano, Alaska, estimated with ice radar and volume modeling

    USGS Publications Warehouse

    Trabant, Dennis C.

    1999-01-01

    The volume of four of the largest glaciers on Iliamna Volcano was estimated using the volume model developed for evaluating glacier volumes on Redoubt Volcano. The volume model is controlled by simulated valley cross sections that are constructed by fitting third-order polynomials to the shape of the valley walls exposed above the glacier surface. Critical cross sections were field checked by sounding with ice-penetrating radar during July 1998. The estimated volumes of perennial snow and glacier ice for Tuxedni, Lateral, Red, and Umbrella Glaciers are 8.6, 0.85, 4.7, and 0.60 cubic kilometers respectively. The estimated volume of snow and ice on the upper 1,000 meters of the volcano is about 1 cubic kilometer. The volume estimates are thought to have errors of no more than ?25 percent. The volumes estimated for the four largest glaciers are more than three times the total volume of snow and ice on Mount Rainier and about 82 times the total volume of snow and ice that was on Mount St. Helens before its May 18, 1980 eruption. Volcanoes mantled by substantial snow and ice covers have produced the largest and most catastrophic lahars and floods. Therefore, it is prudent to expect that, during an eruptive episode, flooding and lahars threaten all of the drainages heading on Iliamna Volcano. On the other hand, debris avalanches can happen any time. Fortunately, their influence is generally limited to the area within a few kilometers of the summit.

  13. Toward a Rapid Synthesis of Field and Desktop Data for Classifying Streams in the Pacific Northwest: Guiding the Sampling and Management of Salmonid Habitat

    NASA Astrophysics Data System (ADS)

    Kasprak, A.; Wheaton, J. M.; Bouwes, N.; Weber, N. P.; Trahan, N. C.; Jordan, C. E.

    2012-12-01

    River managers often seek to understand habitat availability and quality for riverine organisms within the physical template provided by their landscape. Yet the large amount of natural heterogeneity in landscapes gives rise to stream systems which are highly variable over small spatial scales, potentially complicating site selection for surveying aquatic habitat while simultaneously making a simple, wide-reaching management strategy elusive. This is particularly true in the rugged John Day River Basin of northern Oregon, where efforts as part of the Columbia Habitat Monitoring Program to conduct site-based surveys of physical habitat for endangered steelhead salmon (Oncorhynchus mykiss) are underway. As a complete understanding of the type and distribution of habitat available to these fish would require visits to all streams in the basin (impractical due to its large size), here we develop an approach for classifying channel types which combines remote desktop GIS analyses with rapid field-based stream and landscape surveys. At the core of this method, we build off of the River Styles Framework, an open-ended and process-based approach for classifying streams and informing management decisions. This framework is combined with on-the-ground fluvial audits, which aim to quickly and continuously map sediment dynamics and channel behavior along selected channels. Validation of this classification method is completed by on-the-ground stream surveys using a digital iPad platform and by rapid small aircraft overflights to confirm or refine predictions. We further compare this method with existing channel classification approaches for the region (e.g. Beechie, Montgomery and Buffington). The results of this study will help guide both the refinement of site stratification and selection for salmonid habitat monitoring within the basin, and will be vital in designing and prioritizing restoration and management strategies tailored to the distribution of river styles found

  14. 40 CFR 791.48 - Production volume.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 31 2010-07-01 2010-07-01 true Production volume. 791.48 Section 791... (CONTINUED) DATA REIMBURSEMENT Basis for Proposed Order § 791.48 Production volume. (a) Production volume.... (b) For the purpose of determining fair reimbursement shares, production volume shall include amounts...

  15. Fluid Volume Overload and Congestion in Heart Failure: Time to Reconsider Pathophysiology and How Volume Is Assessed.

    PubMed

    Miller, Wayne L

    2016-08-01

    Volume regulation, assessment, and management remain basic issues in patients with heart failure. The discussion presented here is directed at opening a reassessment of the pathophysiology of congestion in congestive heart failure and the methods by which we determine volume overload status. Peer-reviewed historical and contemporary literatures are reviewed. Volume overload and fluid congestion remain primary issues for patients with chronic heart failure. The pathophysiology is complex, and the simple concept of intravascular fluid accumulation is not adequate. The dynamics of interstitial and intravascular fluid compartment interactions and fluid redistribution from venous splanchnic beds to central pulmonary circulation need to be taken into account in strategies of volume management. Clinical bedside evaluations and right heart hemodynamic assessments can alert clinicians of changes in volume status, but only the quantitative measurement of total blood volume can help identify the heterogeneity in plasma volume and red blood cell mass that are features of volume overload in patients with chronic heart failure and help guide individualized, appropriate therapy-not all volume overload is the same. © 2016 American Heart Association, Inc.

  16. Effects of stream channel morphology on golden trout spawning habitat and recruitment.

    Treesearch

    R.A. Knapp; V.T. Vredenburg; K.R. Matthews

    1998-01-01

    Abstract. Populations of stream-dwelling salmonids (e.g., salmon and trout) are generally believed to be regulated by strong density-dependent mortality acting on the age-0 life stage, which produces a dome-shaped stock-recruitment curve. Although this paradigm is based largely on data from anadromous species, it has been widely applied to streamresident salmonids...

  17. 75 FR 5887 - Listing of Color Additives Exempt From Certification; Paracoccus Pigment; Confirmation of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-05

    ... pigment as a color additive in the feed of salmonid fish to enhance the color of their flesh. DATES: The... for the safe use of paracoccus pigment as a color additive in the feed of salmonid fish to enhance the... of Food and Drugs, and redelegated to the Director, Office of Food Additive Safety, notice is given...

  18. Identification of physical habitats limiting the production of coho salmon in western Oregon and Washington.

    Treesearch

    G.H. Reeves; F.H. Everest; T.E. Nickelson

    1989-01-01

    Fishery managers are currently spending millions of dollars per year on habitat enhancement for anadromous salmonids but often do not have the tools needed to ensure success. An analysis of factors limiting production of salmonids in streams must be completed before any habitat-enhancement program is begun. This paper outlines the first formal procedure for identifying...

  19. Development of a prototype fluid volume measurement system. [for urine volume measurement on space missions

    NASA Technical Reports Server (NTRS)

    Poppendiek, H. F.; Sabin, C. M.; Meckel, P. T.

    1974-01-01

    The research is reported in applying the axial fluid temperature differential flowmeter to a urine volume measurement system for space missions. The fluid volume measurement system is described along with the prototype equipment package. Flowmeter calibration, electronic signal processing, and typical void volume measurements are also described.

  20. A model for estimating passive integrated transponder (PIT) tag antenna efficiencies for interval-specific emigration rates

    USGS Publications Warehouse

    Horton, G.E.; Dubreuil, T.L.; Letcher, B.H.

    2007-01-01

    Our goal was to understand movement and its interaction with survival for populations of stream salmonids at long-term study sites in the northeastern United States by employing passive integrated transponder (PIT) tags and associated technology. Although our PIT tag antenna arrays spanned the stream channel (at most flows) and were continuously operated, we are aware that aspects of fish behavior, environmental characteristics, and electronic limitations influenced our ability to detect 100% of the emigration from our stream site. Therefore, we required antenna efficiency estimates to adjust observed emigration rates. We obtained such estimates by testing a full-scale physical model of our PIT tag antenna array in a laboratory setting. From the physical model, we developed a statistical model that we used to predict efficiency in the field. The factors most important for predicting efficiency were external radio frequency signal and tag type. For most sampling intervals, there was concordance between the predicted and observed efficiencies, which allowed us to estimate the true emigration rate for our field populations of tagged salmonids. One caveat is that the model's utility may depend on its ability to characterize external radio frequency signals accurately. Another important consideration is the trade-off between the volume of data necessary to model efficiency accurately and the difficulty of storing and manipulating large amounts of data.

  1. Effects of the liver volume and donor steatosis on errors in the estimated standard liver volume.

    PubMed

    Siriwardana, Rohan Chaminda; Chan, See Ching; Chok, Kenneth Siu Ho; Lo, Chung Mau; Fan, Sheung Tat

    2011-12-01

    An accurate assessment of donor and recipient liver volumes is essential in living donor liver transplantation. Many liver donors are affected by mild to moderate steatosis, and steatotic livers are known to have larger volumes. This study analyzes errors in liver volume estimation by commonly used formulas and the effects of donor steatosis on these errors. Three hundred twenty-five Asian donors who underwent right lobe donor hepatectomy were the subjects of this study. The percentage differences between the liver volumes from computed tomography (CT) and the liver volumes estimated with each formula (ie, the error percentages) were calculated. Five popular formulas were tested. The degrees of steatosis were categorized as follows: no steatosis [n = 178 (54.8%)], ≤ 10% steatosis [n = 128 (39.4%)], and >10% to 20% steatosis [n = 19 (5.8%)]. The median errors ranged from 0.6% (7 mL) to 24.6% (360 mL). The lowest was seen with the locally derived formula. All the formulas showed a significant association between the error percentage and the CT liver volume (P < 0.001). Overestimation was seen with smaller liver volumes, whereas underestimation was seen with larger volumes. The locally derived formula was most accurate when the liver volume was 1001 to 1250 mL. A multivariate analysis showed that the estimation error was dependent on the liver volume (P = 0.001) and the anthropometric measurement that was used in the calculation (P < 0.001) rather than steatosis (P ≥ 0.07). In conclusion, all the formulas have a similar pattern of error that is possibly related to the anthropometric measurement. Clinicians should be aware of this pattern of error and the liver volume with which their formula is most accurate. Copyright © 2011 American Association for the Study of Liver Diseases.

  2. Bibliography of Research on Ocean Fronts, 1964-1984

    DTIC Science & Technology

    1985-08-01

    water masses which exhibit notable differences in temperature, salinity , chlorophyll and other properties. One example of an ocean front is the...Phil. Trans. R. Soc. Lond. A302: 617-634. Amos, A.F., M.G. Langseth and R.G. Markl. 1972. Visible oceanic saline fronts, p. 49-62. In A.L. Gordon...On the mechanism of the deep mixed layer formation during MEDOC 1969. Cahiers Oceanogr. XXII: 427-442. Anderson, F.E. 1980. The variation in

  3. Volume Segmentation and Ghost Particles

    NASA Astrophysics Data System (ADS)

    Ziskin, Isaac; Adrian, Ronald

    2011-11-01

    Volume Segmentation Tomographic PIV (VS-TPIV) is a type of tomographic PIV in which images of particles in a relatively thick volume are segmented into images on a set of much thinner volumes that may be approximated as planes, as in 2D planar PIV. The planes of images can be analysed by standard mono-PIV, and the volume of flow vectors can be recreated by assembling the planes of vectors. The interrogation process is similar to a Holographic PIV analysis, except that the planes of image data are extracted from two-dimensional camera images of the volume of particles instead of three-dimensional holographic images. Like the tomographic PIV method using the MART algorithm, Volume Segmentation requires at least two cameras and works best with three or four. Unlike the MART method, Volume Segmentation does not require reconstruction of individual particle images one pixel at a time and it does not require an iterative process, so it operates much faster. As in all tomographic reconstruction strategies, ambiguities known as ghost particles are produced in the segmentation process. The effect of these ghost particles on the PIV measurement is discussed. This research was supported by Contract 79419-001-09, Los Alamos National Laboratory.

  4. Salmonid Whirling Disease

    USGS Publications Warehouse

    Markiw, Maria E.

    1992-01-01

    Whirling disease is a parasitic infection of trout and salmon by the myxosporean protozoan Myxobolus cerebralis (Syn. Myxosoma cerebralis). This parasite has selective tropism for cartilage; infection can cause deformities of the axial skeleton and neural damage that results in 'blacktail.' The disease is named for the erratic, tail-chasing, 'whirling' in young fish that are startled or fed. Heavy infection of young fish can result in high mortalities or unmarketable, deformed individuals. Although the parasite was first reported in 1903 in central Europe (Hofer 1903), its complete life cycle was not described until the early 1980's.

  5. Wave energy devices with compressible volumes.

    PubMed

    Kurniawan, Adi; Greaves, Deborah; Chaplin, John

    2014-12-08

    We present an analysis of wave energy devices with air-filled compressible submerged volumes, where variability of volume is achieved by means of a horizontal surface free to move up and down relative to the body. An analysis of bodies without power take-off (PTO) systems is first presented to demonstrate the positive effects a compressible volume could have on the body response. Subsequently, two compressible device variations are analysed. In the first variation, the compressible volume is connected to a fixed volume via an air turbine for PTO. In the second variation, a water column separates the compressible volume from another volume, which is fitted with an air turbine open to the atmosphere. Both floating and bottom-fixed, axisymmetric, configurations are considered, and linear analysis is employed throughout. Advantages and disadvantages of each device are examined in detail. Some configurations with displaced volumes less than 2000 m 3 and with constant turbine coefficients are shown to be capable of achieving 80% of the theoretical maximum absorbed power over a wave period range of about 4 s.

  6. Wave energy devices with compressible volumes

    PubMed Central

    Kurniawan, Adi; Greaves, Deborah; Chaplin, John

    2014-01-01

    We present an analysis of wave energy devices with air-filled compressible submerged volumes, where variability of volume is achieved by means of a horizontal surface free to move up and down relative to the body. An analysis of bodies without power take-off (PTO) systems is first presented to demonstrate the positive effects a compressible volume could have on the body response. Subsequently, two compressible device variations are analysed. In the first variation, the compressible volume is connected to a fixed volume via an air turbine for PTO. In the second variation, a water column separates the compressible volume from another volume, which is fitted with an air turbine open to the atmosphere. Both floating and bottom-fixed, axisymmetric, configurations are considered, and linear analysis is employed throughout. Advantages and disadvantages of each device are examined in detail. Some configurations with displaced volumes less than 2000 m3 and with constant turbine coefficients are shown to be capable of achieving 80% of the theoretical maximum absorbed power over a wave period range of about 4 s. PMID:25484609

  7. Boulder Clusters as Flow Refugia for Juvenile Salmonids and Aquatic Invertebrates in Steep Mountain Streams, Klamath Mountains, Northern California

    NASA Astrophysics Data System (ADS)

    Cover, M. R.; May, C. L.; Dietrich, W. E.; Resh, V. H.

    2005-12-01

    The availability of flow refugia and cover is an important factor affecting habitat suitability for fish and invertebrates, especially in steep, turbulent streams. In some channels, crevices beneath and between large rocks may be the only available flow refugia that allow rainbow trout (Oncorhynchus mykiss) to conserve energy and escape from high velocity flow during large storm events. Many aquatic invertebrates, especially large or crawling taxa, require cover that is provided by unembedded crevice space underneath large stones. To investigate the influence of channel type on habitat availability, we performed intensive surveys of crevice habitat for salmonids and benthic invertebrates in 12 reaches in Walker Creek, a 25 square km basin in the Klamath Mountains of Northern California. We identified four reaches in each of three channel types: plane bed (3.1% - 3.7% slope), step-pool (5.4% - 6.5% slope), and cascade (6.3% - 8.5% slope). We used 4 realistic fish models (5, 10, 15, and 20 cm length) to assess the size of crevices and presence of flow refugia associated with all cobble (64 - 256 mm) and boulder (> 256 mm) grains within five 0.5 m-wide diagonal transects. The total abundance of crevices was similar among plane bed (6.3 +/- 1.1 m-2) (Mean +/- SD), step-pool (6.2 +/- 0.25 m-2), and cascade (6.7 +/- 1.2 m-2) reaches. Small (5 cm) crevices made up the majority of crevices in all three reach types. While the presence of 5 cm and 10 cm crevices was not significantly different between the three channel types, there were significantly more large (20 cm) crevices in cascade (0.73 +/- 0.33 m-2) and step-pool (0.68 +/- 0.1 m-2) reaches than in plane bed (0.26 +/- 0.14 m-2) reaches (AVOVA, p < 0.05). Moderately sized (15 cm) crevices were more common in step-pool reaches (0.91 +/- 0.13 m-2) than either cascade (0.54 +/- 0.15 m-2) or plane bed (0.42 +/- 0.13 m-2) reaches. Based on these results we conclude that step-pool reaches provide the most favorable habitat

  8. Survival Estimates for the Passage of Spring-Migrating Juvenile Salmonids through Snake and Columbia River Dams and Reservoirs, 2008.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Faulkner, James R.; Smith, Steven G.; Muir, William D.

    2009-06-23

    In 2008, the National Marine Fisheries Service completed the sixteenth year of a study to estimate survival and travel time of juvenile salmonids Oncorhynchus spp. passing through dams and reservoirs on the Snake and Columbia Rivers. All estimates were derived from detections of fish tagged with passive integrated transponder (PIT) tags. We PIT tagged and released a total of 18,565 hatchery steelhead O. mykiss, 15,991 wild steelhead, and 9,714 wild yearling Chinook salmon O. tshawytscha at Lower Granite Dam in the Snake River. In addition, we utilized fish PIT tagged by other agencies at traps and hatcheries upstream from themore » hydropower system and at sites within the hydropower system in both the Snake and Columbia Rivers. These included 122,061 yearling Chinook salmon tagged at Lower Granite Dam for evaluation of latent mortality related to passage through Snake River dams. PIT-tagged smolts were detected at interrogation facilities at Lower Granite, Little Goose, Lower Monumental, Ice Harbor, McNary, John Day, and Bonneville Dams and in the PIT-tag detector trawl operated in the Columbia River estuary. Survival estimates were calculated using a statistical model for tag-recapture data from single release groups (the single-release model). Primary research objectives in 2008 were to: (1) estimate reach survival and travel time in the Snake and Columbia Rivers throughout the migration period of yearling Chinook salmon and steelhead, (2) evaluate relationships between survival estimates and migration conditions, and (3) evaluate the survival estimation models under prevailing conditions. This report provides reach survival and travel time estimates for 2008 for PIT-tagged yearling Chinook salmon (hatchery and wild), hatchery sockeye salmon O. nerka, hatchery coho salmon O. kisutch, and steelhead (hatchery and wild) in the Snake and Columbia Rivers. Additional details on the methodology and statistical models used are provided in previous reports cited here

  9. Geomagnetism. Volume I

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jacobs, J.A.

    1987-01-01

    The latest attempt to summarise the wealth of knowledge now available on geomagnetic phenomena has resulted in this multi-volume treatise, with contributions and reviews from many scientists. The first volume in the series contains a thorough review of all existing information on measuring the Earth's magnetic field, both on land and at sea, and includes a comparative analysis of the techniques available for this purpose.

  10. REFLECTION AND REFRACTION, VOLUME 2.

    ERIC Educational Resources Information Center

    KLAUS, DAVID J.; AND OTHERS

    THIS VOLUME 2 OF A TWO-VOLUME SET PROVIDES AUTOINSTRUCTION IN PHYSICS. THE UNITS COVERED IN THIS VOLUME ARE (1) REFLECTION OF LIGHT, (2) PHOTOMETRY, (3) POLARIZATION, (4) REFRACTION OF LIGHT, (5) SNELL'S LAW, (6) LENSES, FOCUS, AND FOCAL POINTS, (7) IMAGE FORMATION, AND (8) ABERRATIONS, THE EYE, AND MAGNIFICATION. THE INTRODUCTION AND UNITS ON…

  11. A Comparison of Height-Accumulation and Volume-Equation Methods for Estimating Tree and Stand Volumes

    Treesearch

    R.B. Ferguson; V. Clark Baldwin

    1995-01-01

    Estimating tree and stand volume in mature plantations is time consuming, involving much manpower and equipment; however, several sampling and volume-prediction techniques are available. This study showed that a well-constructed, volume-equation method yields estimates comparable to those of the often more time-consuming, height-accumulation method, even though the...

  12. Assessment and Management of Volume Overload and Congestion in Chronic Heart Failure: Can Measuring Blood Volume Provide New Insights?

    PubMed

    Miller, Wayne L

    2017-01-01

    Volume overload and fluid congestion remain primary clinical challenges in the assessment and management of patients with chronic heart failure (HF). The pathophysiology of volume regulation is complex, and the simple concept of passive intravascular fluid accumulation is not adequate. The dynamics of interstitial and intravascular fluid compartment interactions and fluid redistribution from venous splanchnic beds to the central pulmonary circulation need to be taken into account in strategies of volume management. Clinical bedside evaluations and right heart hemodynamic assessments can alert of changes in volume status, but only the quantitative measurement of total blood volume can help identify the heterogeneity in plasma volume and red blood cell mass that are features of volume overload in chronic HF. The quantitative assessment of intravascular volume is an effective tool to help guide individualized, appropriate therapy. Not all volume overload is the same, and the measurement of intravascular volume identifies heterogeneity to guide tailored therapy.

  13. Genetic Overlap Between Schizophrenia and Volumes of Hippocampus, Putamen, and Intracranial Volume Indicates Shared Molecular Genetic Mechanisms.

    PubMed

    Smeland, Olav B; Wang, Yunpeng; Frei, Oleksandr; Li, Wen; Hibar, Derrek P; Franke, Barbara; Bettella, Francesco; Witoelar, Aree; Djurovic, Srdjan; Chen, Chi-Hua; Thompson, Paul M; Dale, Anders M; Andreassen, Ole A

    2018-06-06

    Schizophrenia (SCZ) is associated with differences in subcortical brain volumes and intracranial volume (ICV). However, little is known about the underlying etiology of these brain alterations. Here, we explored whether brain structure volumes and SCZ share genetic risk factors. Using conditional false discovery rate (FDR) analysis, we integrated genome-wide association study (GWAS) data on SCZ (n = 82315) and GWAS data on 7 subcortical brain volumes and ICV (n = 11840). By conditioning the FDR on overlapping associations, this statistical approach increases power to discover genetic loci. To assess the credibility of our approach, we studied the identified loci in larger GWAS samples on ICV (n = 26577) and hippocampal volume (n = 26814). We observed polygenic overlap between SCZ and volumes of hippocampus, putamen, and ICV. Based on conjunctional FDR < 0.05, we identified 2 loci shared between SCZ and ICV implicating genes FOXO3 (rs10457180) and ITIH4 (rs4687658), 2 loci shared between SCZ and hippocampal volume implicating SLC4A10 (rs4664442) and SPATS2L (rs1653290), and 2 loci shared between SCZ and volume of putamen implicating DCC (rs4632195) and DLG2 (rs11233632). The loci shared between SCZ and hippocampal volume or ICV had not reached significance in the primary GWAS on brain phenotypes. Proving our point of increased power, 2 loci did reach genome-wide significance with ICV (rs10457180) and hippocampal volume (rs4664442) in the larger GWAS. Three of the 6 identified loci are novel for SCZ. Altogether, the findings provide new insights into the relationship between SCZ and brain structure volumes, suggesting that their genetic architectures are not independent.

  14. Rapid Decimation for Direct Volume Rendering

    NASA Technical Reports Server (NTRS)

    Gibbs, Jonathan; VanGelder, Allen; Verma, Vivek; Wilhelms, Jane

    1997-01-01

    An approach for eliminating unnecessary portions of a volume when producing a direct volume rendering is described. This reduction in volume size sacrifices some image quality in the interest of rendering speed. Since volume visualization is often used as an exploratory visualization technique, it is important to reduce rendering times, so the user can effectively explore the volume. The methods presented can speed up rendering by factors of 2 to 3 with minor image degradation. A family of decimation algorithms to reduce the number of primitives in the volume without altering the volume's grid in any way is introduced. This allows the decimation to be computed rapidly, making it easier to change decimation levels on the fly. Further, because very little extra space is required, this method is suitable for the very large volumes that are becoming common. The method is also grid-independent, so it is suitable for multiple overlapping curvilinear and unstructured, as well as regular, grids. The decimation process can proceed automatically, or can be guided by the user so that important regions of the volume are decimated less than unimportant regions. A formal error measure is described based on a three-dimensional analog of the Radon transform. Decimation methods are evaluated based on this metric and on direct comparison with reference images.

  15. Measuring pedestrian volumes and conflicts. Volume III, Measuring pedestrian volumes : a users manual

    DOT National Transportation Integrated Search

    1988-03-01

    Users of the manual are expected to be in divisions responsible for pedestrian safety in cities, counties, and other jurisdictions. The users manual outlines a step-by-step procedure to measure pedestrian volumes using small count intervals. Appendix...

  16. Sex differences in circulatory oxygen transport parameters of sockeye salmon (Oncorhynchus nerka) on the spawning ground.

    PubMed

    Clark, Timothy Darren; Hinch, S G; Taylor, B D; Frappell, P B; Farrell, A P

    2009-07-01

    Upon reaching sexual maturity, several species of male salmonids possess a relative ventricular mass (rM(V)) that may be up to 90% larger than females. This can increase maximum cardiac stroke volume and power output, which may be beneficial to increasing the oxygen transport capacity of male salmonids during the spawning period. It may be further hypothesized, therefore, that other variables within the circulatory oxygen transport cascade, such as blood oxygen-carrying capacity and heart rate, are similarly enhanced in reproductively mature male salmonids. To test this idea, the present study measured a range of circulatory oxygen transport variables in wild male and female sockeye salmon (Oncorhynchus nerka) during their spawning period, following a 150 km migration from the ocean. The rM(V) of male fish was 13% greater than females. Conversely, the haemoglobin concentration ([Hb]) of female fish was 19% higher than males, indicative of a greater blood oxygen-carrying capacity (138 vs. 116 ml O2 l(-1), respectively). Surgically implanted physiological data loggers revealed a similar range in heart rate for both sexes on the spawning ground (20-80 beats min(-1) at 10 degrees C), with a tendency for male fish to spend a greater percentage of time (64%) than females (49%) at heart rates above 50 beats min(-1). Male fish on average consumed significantly more oxygen than females during a 13-h respirometry period. However, routine oxygen consumption rates (.)MO2 ranged between 1.5 and 8.5 mg min(-1) kg(-1) for both sexes, which implies that males did not inherently possess markedly higher routine aerobic energy demands, and suggests that the higher [Hb] of female fish may compensate for the smaller rM(V). These findings reject the hypothesis that all aspects of the circulatory oxygen transport cascade are inherently superior in male sockeye salmon. Instead, it is suggested that any differences in (.)MO2 between sexually mature male and female sockeye salmon can likely

  17. Correction of Age-Related Midface Volume Loss With Low-Volume Hyaluronic Acid Filler.

    PubMed

    Wilson, Monique Vanaman; Fabi, Sabrina Guillen; Greene, Ryan

    2017-03-01

    The pivotal approval trial for a smooth, highly cohesive, viscous, 20-mg/mL hyaluronic acid filler demonstrated sustained aesthetic improvement, with a mean injection volume of 6.65 mL. In daily practice, however, it is not often practical or necessary to use large injection volumes to achieve the desired cosmetic outcome. To assess the efficacy, longevity, and patient satisfaction associated with correction of age-related midface volume loss using the low volumes of hyaluronic acid filler more commonly used in day-to-day practice. A 2-center, retrospective cohort study examined medical records of 61 healthy patients who underwent treatment for facial volume loss with hyaluronic acid filler from November 1, 2013, through April 31, 2014. Follow-up visits were conducted at 1, 3, 6, and 12 months after the procedure. Data were pooled from a private facial plastic surgery practice in Weston, Florida, and a private cosmetic dermatology practice in San Diego, California. Patients were treated with hyaluronic acid filler according to the investigator's usual practices. The main outcome measure was patient-graded Global Aesthetic Improvement Scale scores at 1, 3, 6, and 12 months after treatment. Scores range from 1 to 5; 1 indicates very much improved and 5, worse. A total of 61 consecutive, healthy adult patients (mean [SD] age, 57.4 [12.8] years) with mild to severe facial volume loss were enrolled in the study. A total of 46 patients (75%) were white, 3 (5%) were black/African American, 9 (15%) were Hispanic/Latino, 1 (2%) was Asian/Pacific Islander, and 2 (3%) were other. Three patients (5%) were male, and 58 (95%) were female. Mean initial treatment volume was 1.6 mL. At follow-up, 29 patients (48%) elected to have a touch-up treatment; mean total touch-up volume was 1.4 mL. The patient-graded Global Aesthetic Improvement Scale scores at 1, 3, 6, and 12 months after treatment demonstrated that 73% (41 of 56) to 89% (24 of 27) of the study patients reported being very

  18. The Volume of Earth's Lakes

    NASA Astrophysics Data System (ADS)

    Cael, B. B.

    How much water do lakes on Earth hold? Global lake volume estimates are scarce, highly variable, and poorly documented. We develop a mechanistic null model for estimating global lake mean depth and volume based on a statistical topographic approach to Earth's surface. The volume-area scaling prediction is accurate and consistent within and across lake datasets spanning diverse regions. We applied these relationships to a global lake area census to estimate global lake volume and depth. The volume of Earth's lakes is 199,000 km3 (95% confidence interval 196,000-202,000 km3) . This volume is in the range of historical estimates (166,000-280,000 km3) , but the overall mean depth of 41.8 m (95% CI 41.2-42.4 m) is significantly lower than previous estimates (62 - 151 m). These results highlight and constrain the relative scarcity of lake waters in the hydrosphere and have implications for the role of lakes in global biogeochemical cycles. We also evaluate the size (area) distribution of lakes on Earth compared to expectations from percolation theory. This material is based upon work supported by the National Science Foundation Graduate Research Fellowship Program under Grant No. 2388357.

  19. Basic Quechua. Volume I: Quechua Reader. Volume II: Quechua Grammar and Dictionary.

    ERIC Educational Resources Information Center

    Aitken-Soux, Percy G.; Crapo, Richley H.

    Volume I, the reader, has 86 lessons consisting of short passages and vocabulary lists. The language and the stories presented were learned and collected at the Indian community and Hacienda of Cayara near Potosi, Bolivia. Translations of the passages are provided in a separate section. The second volume presents the grammar and phonology of the…

  20. The Comprehensive Health Challenge: Promoting Health through Education. Volume One; Volume Two.

    ERIC Educational Resources Information Center

    Cortese, Peter, Ed.; Middleton, Kathleen, Ed.

    The 32 chapters in this book (presented in two volumes) cover a continuum of issues in comprehensive school health education, including a review of the past and a vision of the future. Volume 1 opens with a foreword by Dr. M. Jocelyn Elders (Surgeon General of the United States) and provides the following chapters: (1) "School Health…

  1. Development of a nested polymerase chain reaction for amplification of a sequence of the p57 gene of Renibacterium salmoninarum that provides a highly sensitive method for detection of the bacterium in salmonid kidney

    USGS Publications Warehouse

    Chase, D.M.; Pascho, R.J.

    1998-01-01

    Nucleic acid-based assays have shown promise for diagnosing Renibacterium salmoninarum in tissues and body fluids of salmonids. DeVelopment of a nested polymerase chain reaction (PCR) method to detect a 320 bp DNA segment of the gene encoding the p57 protein of R. salmoninarum is described. Whereas a conventional PCR for a 383 bp segment of the p57 gene reliably detected 1000 R. salmoninarum cells per reaction in kidney tissue, the nested PCR detected as few as 10 R. salmoninarum per reaction in kidney tissue. Two DNA extraction methods for the nested PCR were compared and the correlation between replicate samples was generally higher in samples extracted by the QIAamp system compared with those extracted by the phenol/chloroform method. The specificity of the nested PCR was confirmed by testing DNA extracts of common bacterial fish pathogens and a panel of bacterial species reported to cause false-positive reactions in the enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody test (FAT) for R. salmoninarum. Kidney samples from 74 naturally infected chinook Salmon were examined by the nested PCR, the ELISA, and the FAT, and the detected prevalences of R. salmoninarum were 61, 47, and 43%, respectively.

  2. Amygdala volumes in childhood absence epilepsy.

    PubMed

    Schreibman Cohen, Ayelet; Daley, Melita; Siddarth, Prabha; Levitt, Jennifer; Loesch, Ingrid K; Altshuler, Lori; Ly, Ronald; Shields, W Donald; Gurbani, Suresh; Caplan, Rochelle

    2009-11-01

    Abnormal amygdala volumes in pediatric mood-anxiety disorders and attention deficit hyperactivity disorder (ADHD), as well as high rates of these diagnoses in childhood absence epilepsy (CAE), prompted this study of amygdala volume in CAE. Twenty-six children with CAE and 23 normal children, aged 6.6-15.8 years, underwent MRI at 1.5 T. The tissue imaged with MRI was segmented, and amygdala volumes were obtained by manual tracings. There were no significant amygdala volume differences between the CAE and normal groups. Within the CAE group, however, the children with ADHD had significantly smaller amygdala volumes than the subjects with CAE with no psychopathology and those with mood/anxiety diagnoses. There was also a significant relationship between higher seizure frequency and greater amygdala asymmetry in the epilepsy group. Given ongoing development of the amygdala during late childhood and adolescence, despite the lack of significant group differences in amygdala volumes, the association of amygdala volume abnormalities with ADHD and seizure frequency implies a possible impact of the disorder on amygdala development and CAE-associated comorbidities, such as ADHD.

  3. Geometric convex cone volume analysis

    NASA Astrophysics Data System (ADS)

    Li, Hsiao-Chi; Chang, Chein-I.

    2016-05-01

    Convexity is a major concept used to design and develop endmember finding algorithms (EFAs). For abundance unconstrained techniques, Pixel Purity Index (PPI) and Automatic Target Generation Process (ATGP) which use Orthogonal Projection (OP) as a criterion, are commonly used method. For abundance partially constrained techniques, Convex Cone Analysis is generally preferred which makes use of convex cones to impose Abundance Non-negativity Constraint (ANC). For abundance fully constrained N-FINDR and Simplex Growing Algorithm (SGA) are most popular methods which use simplex volume as a criterion to impose ANC and Abundance Sum-to-one Constraint (ASC). This paper analyze an issue encountered in volume calculation with a hyperplane introduced to illustrate an idea of bounded convex cone. Geometric Convex Cone Volume Analysis (GCCVA) projects the boundary vectors of a convex cone orthogonally on a hyperplane to reduce the effect of background signatures and a geometric volume approach is applied to address the issue arose from calculating volume and further improve the performance of convex cone-based EFAs.

  4. Diel resource partitioning among juvenile Atlantic Salmon, Brown Trout, and Rainbow Trout during summer

    USGS Publications Warehouse

    Johnson, James H.; McKenna, James E.

    2015-01-01

    Interspecific partitioning of food and habitat resources has been widely studied in stream salmonids. Most studies have examined resource partitioning between two native species or between a native species and one that has been introduced. In this study we examine the diel feeding ecology and habitat use of three species of juvenile salmonids (i.e., Atlantic Salmon Salmo salar, Brown Trout Salmo trutta, and Rainbow Trout Oncorhynchus mykiss) in a tributary of Skaneateles Lake, New York. Subyearling Brown Trout and Rainbow Trout fed more heavily from the drift than the benthos, whereas subyearling Atlantic Salmon fed more from the benthos than either species of trout. Feeding activity of Atlantic Salmon and Rainbow Trout was similar, with both species increasing feeding at dusk, whereas Brown Trout had no discernable feeding peak or trough. Habitat availability was important in determining site-specific habitat use by juvenile salmonids. Habitat selection was greater during the day than at night. The intrastream, diel, intraspecific, and interspecific variation we observed in salmonid habitat use in Grout Brook illustrates the difficulty of acquiring habitat use information for widespread management applications.

  5. Fast detection of Piscirickettsia salmonis in Salmo salar serum through MALDI-TOF-MS profiling.

    PubMed

    Olate, Verónica R; Nachtigall, Fabiane M; Santos, Leonardo S; Soto, Alex; Araya, Macarena; Oyanedel, Sandra; Díaz, Verónica; Marchant, Vanessa; Rios-Momberg, Mauricio

    2016-03-01

    Piscirickettsia salmonis is a pathogenic bacteria known as the aetiological agent of the salmonid rickettsial syndrome and causes a high mortality in farmed salmonid fishes. Detection of P. salmonis in farmed fishes is based mainly on molecular biology and immunohistochemistry techniques. These techniques are in most of the cases expensive and time consuming. In the search of new alternatives to detect the presence of P. salmonis in salmonid fishes, this work proposed the use of MALDI-TOF-MS to compare serum protein profiles from Salmo salar fish, including experimentally infected and non-infected fishes using principal component analysis (PCA). Samples were obtained from a controlled bioassay where S. salar was challenged with P. salmonis in a cohabitation model and classified according to the presence or absence of the bacteria by real time PCR analysis. MALDI spectra of the fish serum samples showed differences in its serum protein composition. These differences were corroborated with PCA analysis. The results demonstrated that the use of both MALDI-TOF-MS and PCA represents a useful tool to discriminate the fish status through the analysis of salmonid serum samples. Copyright © 2016 John Wiley & Sons, Ltd.

  6. Data collection system. Volume 1, Overview and operators manual; Volume 2, Maintenance manual; Appendices

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Caudell, R.B.; Bauder, M.E.; Boyer, W.B.

    1993-09-01

    Sandia National Laboratories (SNL) Instrumentation Development Department was tasked by the Defense Nuclear Agency (DNA) to record data on Tektronix RTD720 Digitizers on the HUNTERS TROPHY field test conducted at the Nevada Test Site (NTS) on September 18, 1992. This report contains a overview and description of the computer hardware and software that was used to acquire, reduce, and display the data. The document is divided into two volumes: an overview and operators manual (Volume 1) and a maintenance manual (Volume 2).

  7. Volumetric measurement of tank volume

    NASA Technical Reports Server (NTRS)

    Walter, Richard T. (Inventor); Vanbuskirk, Paul D. (Inventor); Weber, William F. (Inventor); Froebel, Richard C. (Inventor)

    1991-01-01

    A method is disclosed for determining the volume of compressible gas in a system including incompressible substances in a zero-gravity environment consisting of measuring the change in pressure (delta P) for a known volume change rate (delta V/delta t) in the polytrophic region between isothermal and adiabatic conditions. The measurements are utilized in an idealized formula for determining the change in isothermal pressure (delta P sub iso) for the gas. From the isothermal pressure change (delta iso) the gas volume is obtained. The method is also applicable to determination of gas volume by utilizing work (W) in the compression process. In a passive system, the relationship of specific densities can be obtained.

  8. Italian biophysics and SIBPA speed-up the pace towards the long and winding road of the interdisciplinary science.

    PubMed

    Giacomazza, Daniela; Musio, Carlo

    2016-01-01

    This Special Issue of Biophysical Chemistry presents a selection of the contributions presented at the XXII National Congress of the Italian Society of Pure and Applied Biophysics (i.e., SIBPA, Società Italiana di Biofisica Pura ed Applicata) held on September 2014 in Palermo, Italy. Topics cover all biophysical disciplines, from molecular to cellular, to integrative biophysics giving a comprehensive view of the inter- and multi-disciplinary approach of modern biophysics. SIBPA, which turned 40 in 2013, continues to grow and attract interest.

  9. E-2 Systems Approach to Training: Development, Implementation, Evaluation, and Revision.

    DTIC Science & Technology

    1979-08-01

    1 1 2.0 xxII 2 2 3.3 XXIII 5 1.5 2 3.3 XXIV 1 2 1.5 2.- XXV 4 1 39 NAVTRAEQUIPCEN 78-C-0045-1 TABLE 2. (CONTINUED) lOIIhLI CARRIL CLASSROOM 1 SI-8 - 2...i i cvc r ed i -i a e ext reP me ly tnt e r,, t I n ______ b_ h. n te re s tinr% __________c. bo r ing _________d. extreme-ly bu )ring o. .IA 1

  10. Lung volumes: measurement, clinical use, and coding.

    PubMed

    Flesch, Judd D; Dine, C Jessica

    2012-08-01

    Measurement of lung volumes is an integral part of complete pulmonary function testing. Some lung volumes can be measured during spirometry; however, measurement of the residual volume (RV), functional residual capacity (FRC), and total lung capacity (TLC) requires special techniques. FRC is typically measured by one of three methods. Body plethysmography uses Boyle's Law to determine lung volumes, whereas inert gas dilution and nitrogen washout use dilution properties of gases. After determination of FRC, expiratory reserve volume and inspiratory vital capacity are measured, which allows the calculation of the RV and TLC. Lung volumes are commonly used for the diagnosis of restriction. In obstructive lung disease, they are used to assess for hyperinflation. Changes in lung volumes can also be seen in a number of other clinical conditions. Reimbursement for measurement of lung volumes requires knowledge of current procedural terminology (CPT) codes, relevant indications, and an appropriate level of physician supervision. Because of recent efforts to eliminate payment inefficiencies, the 10 previous CPT codes for lung volumes, airway resistance, and diffusing capacity have been bundled into four new CPT codes.

  11. Lung volumes in giraffes, Giraffa camelopardalis.

    PubMed

    Mitchell, G; Skinner, J D

    2011-01-01

    We have measured lung mass and trachea dimensions in 46 giraffes of both genders ranging in body mass from 147 kg to 1441 kg, calculated static and dynamic lung volumes, and developed allometric equations that relate changes in them to growth. We found that relative lung mass is 0.6±0.2% of body mass which is significantly less than it is in other mammals (1.1±0.1%). Total lung volume is significantly smaller (46.2±5.9 mL kg⁻¹) than in similar sized mammals (75.0±2.1 mL kg⁻¹). The lung volume:body mass ratio decreases during growth rather than increase as it does in other mammals. Tracheal diameter is significantly narrower than in similar sized mammals but dead space volume (2.9±0.5 mL kg⁻¹) is larger than in similar sized mammals (2.4±0.1 mL kg⁻¹). Our calculations suggest that tidal volume (10.5±0.2 mL kg⁻¹) is increased compared to that in other mammals(10.0±0.2 mL kg⁻¹) so that the dead space:tidal volume ratio is the same as in other mammals. Calculated Functional Residual Capacity is smaller than predicted (53.4±3.5 vs 33.7±0.6 mL kg⁻¹) as is Expiratory Reserve Volume (47.4±2.6 vs 27.2±1.0 mL kg⁻¹, but Residual Volume (6.0±0.4 mL kg⁻¹) is the same as in other similar sized mammals (6.0±0.9 mL kg⁻¹. Our calculations suggest that Inspiratory Reserve Volume is significantly reduced in size (11.6±1.6 vs 3.8±2.4 mL kg⁻¹), and, if so, the capacity to increase tidal volume is limited. Calculated dynamic lung volumes were the same as in similar sized mammals. We have concluded that giraffe morphology has resulted in lung volumes that are significantly different to that of similar sized mammals, but these changes do not compromise ventilatory capacity. Copyright © 2010 Elsevier Inc. All rights reserved.

  12. 21 CFR 868.1760 - Volume plethysmograph.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... the patient's lung volume changes. (b) Classification. Class II (performance standards). ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Volume plethysmograph. 868.1760 Section 868.1760...) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Diagnostic Devices § 868.1760 Volume plethysmograph. (a...

  13. Lineage-specific rediploidization is a mechanism to explain time-lags between genome duplication and evolutionary diversification.

    PubMed

    Robertson, Fiona M; Gundappa, Manu Kumar; Grammes, Fabian; Hvidsten, Torgeir R; Redmond, Anthony K; Lien, Sigbjørn; Martin, Samuel A M; Holland, Peter W H; Sandve, Simen R; Macqueen, Daniel J

    2017-06-14

    The functional divergence of duplicate genes (ohnologues) retained from whole genome duplication (WGD) is thought to promote evolutionary diversification. However, species radiation and phenotypic diversification are often temporally separated from WGD. Salmonid fish, whose ancestor underwent WGD by autotetraploidization ~95 million years ago, fit such a 'time-lag' model of post-WGD radiation, which occurred alongside a major delay in the rediploidization process. Here we propose a model, 'lineage-specific ohnologue resolution' (LORe), to address the consequences of delayed rediploidization. Under LORe, speciation precedes rediploidization, allowing independent ohnologue divergence in sister lineages sharing an ancestral WGD event. Using cross-species sequence capture, phylogenomics and genome-wide analyses of ohnologue expression divergence, we demonstrate the major impact of LORe on salmonid evolution. One-quarter of each salmonid genome, harbouring at least 4550 ohnologues, has evolved under LORe, with rediploidization and functional divergence occurring on multiple independent occasions >50 million years post-WGD. We demonstrate the existence and regulatory divergence of many LORe ohnologues with functions in lineage-specific physiological adaptations that potentially facilitated salmonid species radiation. We show that LORe ohnologues are enriched for different functions than 'older' ohnologues that began diverging in the salmonid ancestor. LORe has unappreciated significance as a nested component of post-WGD divergence that impacts the functional properties of genes, whilst providing ohnologues available solely for lineage-specific adaptation. Under LORe, which is predicted following many WGD events, the functional outcomes of WGD need not appear 'explosively', but can arise gradually over tens of millions of years, promoting lineage-specific diversification regimes under prevailing ecological pressures.

  14. Predation on juvenile pacific salmon oncorhynchus spp. in downstream migrant traps in prairie creek, california

    USGS Publications Warehouse

    Duffy, W.G.; Bjorkstedt, E.P.; Ellings, C.S.

    2011-01-01

    Downstream migrant traps are a widely applied fishery management tool for sampling anadromous Pacific salmon Oncorhynchus spp. and steelhead O. mykiss smolts along theWest Coast of North America and elsewhere, yet predation on juvenile salmonids in traps has not been studied quantitatively.We assessed the frequency of occurrence and abundance of juvenile salmonids in the stomachs of coastal cutthroat trout O. clarkii clarkii, coho salmon O. kisutch, steelhead, and prickly sculpin Cottus asper (>70 mm fork length) captured in traps and in nearby stream habitats. All four predator species took juvenile salmonids with much greater frequency in traps than in stream habitats. Among free-swimming predators, only coastal cutthroat trout were observed with salmonid fry in their stomachs, but they took fewer salmonid prey and appeared to rely more heavily on insect prey than did coastal cutthroat trout captured in traps. Predators consumed up to 25% of the available prey over a broad range of prey abundances. Over the course of the study, predators consumed 2.5% of all salmonid fry captured in traps, but this fraction ranged from less than 1% to more than 10% in any given year. The number of prey taken in traps increased with predator length and with prey abundance in traps, and predation in traps peaked during the period of most intense downstream migration by salmon fry. In contrast, live-box design and trap location had little or no effect on the total number of prey taken by individual predators.We estimated that the predation mortality of juvenile salmon increased by 0.5-1.0% due to in-trap predation (i.e., a 9-10% relative increase over natural predation rates). We found no evidence that predators selected for prey on the basis of species. These results should motivate additional research on methods that reduce or eliminate predation in trap live-boxes and protocols for efficiently measuring predation associated with the trapping of downstream migrants. ?? American

  15. Recurrence interval analysis of trading volumes

    NASA Astrophysics Data System (ADS)

    Ren, Fei; Zhou, Wei-Xing

    2010-06-01

    We study the statistical properties of the recurrence intervals τ between successive trading volumes exceeding a certain threshold q . The recurrence interval analysis is carried out for the 20 liquid Chinese stocks covering a period from January 2000 to May 2009, and two Chinese indices from January 2003 to April 2009. Similar to the recurrence interval distribution of the price returns, the tail of the recurrence interval distribution of the trading volumes follows a power-law scaling, and the results are verified by the goodness-of-fit tests using the Kolmogorov-Smirnov (KS) statistic, the weighted KS statistic and the Cramér-von Mises criterion. The measurements of the conditional probability distribution and the detrended fluctuation function show that both short-term and long-term memory effects exist in the recurrence intervals between trading volumes. We further study the relationship between trading volumes and price returns based on the recurrence interval analysis method. It is found that large trading volumes are more likely to occur following large price returns, and the comovement between trading volumes and price returns is more pronounced for large trading volumes.

  16. Recurrence interval analysis of trading volumes.

    PubMed

    Ren, Fei; Zhou, Wei-Xing

    2010-06-01

    We study the statistical properties of the recurrence intervals τ between successive trading volumes exceeding a certain threshold q. The recurrence interval analysis is carried out for the 20 liquid Chinese stocks covering a period from January 2000 to May 2009, and two Chinese indices from January 2003 to April 2009. Similar to the recurrence interval distribution of the price returns, the tail of the recurrence interval distribution of the trading volumes follows a power-law scaling, and the results are verified by the goodness-of-fit tests using the Kolmogorov-Smirnov (KS) statistic, the weighted KS statistic and the Cramér-von Mises criterion. The measurements of the conditional probability distribution and the detrended fluctuation function show that both short-term and long-term memory effects exist in the recurrence intervals between trading volumes. We further study the relationship between trading volumes and price returns based on the recurrence interval analysis method. It is found that large trading volumes are more likely to occur following large price returns, and the comovement between trading volumes and price returns is more pronounced for large trading volumes.

  17. Speckle Interferometry at the U.S. Naval Observatory. XXII.

    NASA Astrophysics Data System (ADS)

    Mason, Brian D.; Hartkopf, William I.

    2017-11-01

    The results of 4747 intensified CCD observations of double stars, made with the 26 inch refractor of the U.S. Naval Observatory, are presented. Each observation of a system represents a combination of over 2000 short-exposure images. These observations are averaged into 2667 mean relative positions and range in separation from 0.″328 to 95.″9, with a median separation of 8.″673. Eight orbits are improved. This is the 22nd in this series of papers and covers the period 2016 January 4 through 2016 December 29.

  18. PREFACE: XXII International Conference on Spectral Line Shapes 2014

    NASA Astrophysics Data System (ADS)

    Parigger, C. G.

    2014-11-01

    The 22nd International Conference on Spectral Line Shapes (ICSLS) was convened at The University of Tennessee Space Institute (UTSI) at Tullahoma, Tennessee, USA, during June 1 to 6, 2014. A variety of topics of interest to the line shape community were addressed during invited and contributed oral and poster presentations. General categories of the ICSLS 2014 scientific contents included Astrophysics, Biomedical Physics, High and Low Temperature Plasma Physics, Magnetic Fusion Physics, Neutrals Atomic-Molecular-Optical (AMO) Physics, and Applied Physics. Research interests at UTSI and at the Center for Laser Applications (CLA) focus on Applied Physics and Plasma Physics areas such as laser-induced breakdown spectroscopy, spectroscopy with ultra-short light pulses, combustion diagnostics, to name a few. Consequently, the presentations during the conference addressed a variety of these topics. Attendance at the conference included researchers from North America, Africa, Asia and Europe, with an international representation showing 250 authors and co-authors with over 25 different citizenships, and 100 participants at the Conference. Figure 1 shows a photo of Conference attendees. The schedule included 82 contributions, 41 oral and 41 poster presentations. The 29 invited, 12 contributed oral and 41 contributed poster presentations were selected following communication with the international organizing committee members. A smart phone ''app'' was also utilized, thanks to Elsevier, to communicate electronic versions of the posters during the conference. Special thanks go to the members of the international and local committees for their work in organizing the 22nd ICSLS. In addition, thank you notes also go to the peer reviewers for the proceedings. Following the success of the IOP: Journal of Physics Conference Series selected for the 21st ICSLS publication, the proceedings papers report ongoing research activities. Papers submitted amount to 68 in number, or 83% of the 82 papers contributed to the 22nd ICSLS conference will be published in the IOP: Journal of Physics proceedings. The Executive Director of the University of Tennessee Space Institute welcomed all participants of the Conference on the first day of the technical sessions on Monday June 2, 2014. This welcome address is also included in the conference series publication, especially important for Physics and Engineering research at UTSI is the concurrent 50-year celebration of the Institute in 2014. Informal welcome occurred on Sunday June 1, 2014, and various social activities included a tour to the Jack Daniel's distillery in Lynchburg, Tennessee, followed by the conference dinner. The international scientific committee met to look into various aspects of the ICSLS and future role of this conference for the spectral line shape community. The next meeting locations have been discussed, including the scheduling of the next 23rd conference in Torun, Poland, in June of 2016. Further meeting locations include hosting the conference in Egypt in 2018, possibly in Luxor, Egypt. Communication regarding the 24th ICSLS in 2020 included mentioning of scheduling the Conference to occur in Dublin, Ireland. Clearly, there is a wealth of interest in continuing the long standing tradition of communicating spectral signatures and line shapes at the biannual ICSLS meetings. The 22nd International Conference on Spectral Line Shapes was supported by the Institute of Physics, the University of Tennessee Space Institute, the Center for Laser Applications, the Quantel Laser company, and by Elsevier. On behalf of the organizing committee, I greatly appreciate the support.

  19. Occurrence and distribution of pesticides in surface waters of the Hood River basin, Oregon, 1999-2009

    USGS Publications Warehouse

    Temple, Whitney B.; Johnson, Henry M.

    2011-01-01

    The U.S. Geological Survey analyzed pesticide and trace-element concentration data from the Hood River basin collected by the Oregon Department of Environmental Quality (ODEQ) from 1999 through 2009 to determine the distribution and concentrations of pesticides in the basin's surface waters. Instream concentrations were compared to (1) national and State water-quality standards established to protect aquatic organisms and (2) concentrations that cause sublethal or lethal effects in order to assess their potential to adversely affect the health of salmonids and their prey organisms. Three salmonid species native to the basin are listed as "threatened" under the U.S. Endangered Species Act: bull trout, steelhead, and Chinook salmon. A subset of 16 sites was sampled every year by the ODEQ for pesticides, with sample collection targeted to months of peak pesticide use in orchards (March-June and September). Ten pesticides and four pesticide degradation products were analyzed from 1999 through 2008; 100 were analyzed in 2009. Nineteen pesticides were detected: 11 insecticides, 6 herbicides, and 2 fungicides. Two of four insecticide degradation products were detected. All five detected organophosphate insecticides and the one detected organochlorine insecticide were present at concentrations exceeding water-quality standards, sublethal effects thresholds, or acute toxicity values in one or more samples. The frequency of organophosphate detection in the basin decreased during the period of record; however, changes in sampling schedule and laboratory reporting limits hindered clear analysis of detection frequency trends. Detected herbicide and fungicide concentrations were less than water-quality standards, sublethal effects thresholds, or acute toxicity values. Simazine, the most frequently detected pesticide, was the only herbicide detected at concentrations within an order of magnitude (factor of 10) of concentrations that impact salmonid olfaction. Some detected

  20. Feeding Activity, Rate of Consumption, Daily Ration and Prey Selection of Major Predators in the John Day Pool. 1982 Annual Report.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gray, Gerard A.; United States. Bonneville Power Administration

    1984-03-01

    This study was initiated to determine the extent of predation by resident populations of native and introduced fish on juvenile salmonids in main stem Columbia River Reservoirs. The John Day Reservoir and tailrace was selected as the study area. First year objectives were: (1) determine whether native and introduced predators preyed on juvenile salmonids; (2) determine which species were major predators; and (3) locate areas where predation was most intense. Results indicated that juvenile salmonids were consumed by all four predatory fish species studied: northern squawfish (Ptychocheilus oregonensis), walleye (Stizostedion vitreum vitreum), smallmouth bass (Micropterus dolomieu), and channel catfish (Ictalurusmore » punctatus). However, degree of predation varied among predators as a function of spatial distribution, apparent abundance, size, and temporal feeding behavior. 15 figs., 16 tabs.« less

  1. Comparison of traditional and molecular methods for detection of Renibacterium salmoninarum

    USGS Publications Warehouse

    Pascho, R.J.; Elliott, D.G.; Chase, D.M.; Cunningham, C.O.

    2002-01-01

    Bacterial kidney disease (BKD) caused by Renibacterium salmoninarum occurs in most parts of the world where wild or cultured salmonid fishes are present. Several extensive reviews have been written on the pathogen and the disease (Fryer and Sanders, 1981; Austin and Austin, 1987; Elliott et al., 1989; Evelyn, 1993; Evenden et al., 1993; Fryer and Lannan, 1993). Bacterial kidney disease can cause serious mortality in juvenile salmonids in both fresh water and seawater, and also in prespawning adults. Although the chronic nature of the disease has hindered accurate estimates of fish losses, particularly in feral fish populations, BKD is one the most important bacterial diseases affecting cultured salmonids, with reported losses as high as 80% in stocks of Pacific salmon (Oncorhynchus spp.) and 40% in stocks of Atlantic salmon (Salmo salar) (Evenden et al., 1993).

  2. 42 CFR 495.306 - Establishing patient volume.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... calculate patient volume at the group practice/clinic level, but only in accordance with all of the following limitations: (1) The clinic or group practice's patient volume is appropriate as a patient volume... practice's patient volume determination. (3) All EPs in the group practice or clinic must use the same...

  3. Fluoride Induces a Volume Reduction in CA1 Hippocampal Slices Via MAP Kinase Pathway Through Volume Regulated Anion Channels

    PubMed Central

    Lee, Jaekwang; Han, Young-Eun; Favorov, Oleg; Tommerdahl, Mark; Whitsel, Barry

    2016-01-01

    Regulation of cell volume is an important aspect of cellular homeostasis during neural activity. This volume regulation is thought to be mediated by activation of specific transporters, aquaporin, and volume regulated anion channels (VRAC). In cultured astrocytes, it was reported that swelling-induced mitogen-activated protein (MAP) kinase activation is required to open VRAC, which are thought to be important in regulatory volume decrease and in the response of CNS to trauma and excitotoxicity. It has been also described that sodium fluoride (NaF), a recognized G-protein activator and protein phosphatase inhibitor, leads to a significant MAP kinase activation in endothelial cells. However, NaF's effect in volume regulation in the brain is not known yet. Here, we investigated the mechanism of NaF-induced volume change in rat and mouse hippocampal slices using intrinsic optical signal (IOS) recording, in which we measured relative changes in intracellular and extracellular volume as changes in light transmittance through brain slices. We found that NaF (1~5 mM) application induced a reduction in light transmittance (decreased volume) in CA1 hippocampus, which was completely reversed by MAP kinase inhibitor U0126 (10 µM). We also observed that NaF-induced volume reduction was blocked by anion channel blockers, suggesting that NaF-induced volume reduction could be mediated by VRAC. Overall, our results propose a novel molecular mechanism of NaF-induced volume reduction via MAP kinase signaling pathway by activation of VRAC. PMID:27122993

  4. Factors influencing the difference between forecasted and actual drug sales volumes under the price-volume agreement in South Korea.

    PubMed

    Park, Sun-Young; Han, Euna; Kim, Jini; Lee, Eui-Kyung

    2016-08-01

    This study analyzed factors contributing to increases in the actual sales volumes relative to forecasted volumes of drugs under price-volume agreement (PVA) policy in South Korea. Sales volumes of newly listed drugs on the national formulary are monitored under PVA policy. When actual sales volume exceeds the pre-agreed forecasted volume by 30% or more, the drug is subject to price-reduction. Logistic regression assessed the factors related to whether drugs were the PVA price-reduction drugs. A generalized linear model with gamma distribution and log-link assessed the factors influencing the increase in actual volumes compared to forecasted volume in the PVA price-reduction drugs. Of 186 PVA monitored drugs, 34.9% were price-reduction drugs. Drugs marketed by pharmaceutical companies with previous-occupation in the therapeutic markets were more likely to be PVA price-reduction drugs than drugs marketed by firms with no previous-occupation. Drugs of multinational pharmaceutical companies were more likely to be PVA price-reduction drugs than those of domestic companies. Having more alternative existing drugs was significantly associated with higher odds of being PVA price-reduction drugs. Among the PVA price-reduction drugs, the increasing rate of actual volume compared to forecasted volume was significantly higher in drugs with clinical usefulness. By focusing the negotiation efforts on those target drugs, PVA policy can be administered more efficiently with the improved predictability of the drug sales volumes. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  5. A Finite-Volume "Shaving" Method for Interfacing NASA/DAO''s Physical Space Statistical Analysis System to the Finite-Volume GCM with a Lagrangian Control-Volume Vertical Coordinate

    NASA Technical Reports Server (NTRS)

    Lin, Shian-Jiann; DaSilva, Arlindo; Atlas, Robert (Technical Monitor)

    2001-01-01

    Toward the development of a finite-volume Data Assimilation System (fvDAS), a consistent finite-volume methodology is developed for interfacing the NASA/DAO's Physical Space Statistical Analysis System (PSAS) to the joint NASA/NCAR finite volume CCM3 (fvCCM3). To take advantage of the Lagrangian control-volume vertical coordinate of the fvCCM3, a novel "shaving" method is applied to the lowest few model layers to reflect the surface pressure changes as implied by the final analysis. Analysis increments (from PSAS) to the upper air variables are then consistently put onto the Lagrangian layers as adjustments to the volume-mean quantities during the analysis cycle. This approach is demonstrated to be superior to the conventional method of using independently computed "tendency terms" for surface pressure and upper air prognostic variables.

  6. Assessment of smolt condition for travel time analysis. Annual report 1989

    USGS Publications Warehouse

    Beeman, J.W.; Rondorf, D.W.; Faler, J.C.; Free, M.E.; Haner, P.V.

    1990-01-01

    The Water Budget is a volume of water used to enhance environmental conditions (flows) in the Columbia and Snake rivers for juvenile salmonids during their seaward migration. To manage the Water Budget, the Fish Passage Center estimates travel times of juvenile salmonids in index reaches of the main-stem rivers, using information on river flows and the migrational characteristics of the juvenile salmonids. This study was initiated to provide physiological information on the juvenile salmonids used for these travel time estimates. The physiological ability to respond to stressors was evaluated by measuring concentrations of plasma cortisol, glucose, and chlorides before and after a 30-s handling-stress challenge test. As in 1988, most groups responded satisfactorily to the challenge. The scope for response was compromised among two groups of juvenile chinook salmon that were trucked to release sites and in steelhead from one hatchery after unusual marking and transportation protocols were used. The development of smoltification was assessed by measuring gill Na+-K+ ATPase activity and plasma thyroxine concentrations. Mean ATPase activities of marked hatchery groups of juvenile chinook salmon and steelhead changed little during the month before release and rose sharply for about the first 20 d of the migration after release. Mean plasma thyroxine was highest during the first 20 d after release. Mean gill ATPase activity of spring chinook salmon from the migration-at-large peaked at about the 90th percentile of passage at Rock Island and Lower Granite dams, and at about the 50th percentile of passage at McNary Dam. Mean gill ATPase activity of wild steelhead was higher than gill ATPase activity of hatchery steelhead at Rock Island Dam, the Snake River Trap, and Lower Granite Dam, but not at McNary Dam. This was attributed to a time-dependent relationship between increases in ATPase activity and the number of days fish migrated before recapture. Correlations of gill

  7. Timber Volume in Indiana, 1967.

    Treesearch

    Arnold J. Ostrom

    1969-01-01

    The recently completed Second Forest Survey of Indiana shows that the State's timber volume has increased by one-fourth in the 17 years since the previous survey. Timber volume by county is presented.

  8. APOL1 renal-risk variants associate with reduced cerebral white matter lesion volume and increased gray matter volume.

    PubMed

    Freedman, Barry I; Gadegbeku, Crystal A; Bryan, R Nick; Palmer, Nicholette D; Hicks, Pamela J; Ma, Lijun; Rocco, Michael V; Smith, S Carrie; Xu, Jianzhao; Whitlow, Christopher T; Wagner, Benjamin C; Langefeld, Carl D; Hawfield, Amret T; Bates, Jeffrey T; Lerner, Alan J; Raj, Dominic S; Sadaghiani, Mohammad S; Toto, Robert D; Wright, Jackson T; Bowden, Donald W; Williamson, Jeff D; Sink, Kaycee M; Maldjian, Joseph A; Pajewski, Nicholas M; Divers, Jasmin

    2016-08-01

    To assess apolipoprotein L1 gene (APOL1) renal-risk-variant effects on the brain, magnetic resonance imaging (MRI)-based cerebral volumes and cognitive function were assessed in 517 African American-Diabetes Heart Study (AA-DHS) Memory IN Diabetes (MIND) and 2568 hypertensive African American Systolic Blood Pressure Intervention Trial (SPRINT) participants without diabetes. Within these cohorts, 483 and 197 had cerebral MRI, respectively. AA-DHS participants were characterized as follows: 60.9% female, mean age of 58.6 years, diabetes duration 13.1 years, estimated glomerular filtration rate of 88.2 ml/min/1.73 m(2), and a median spot urine albumin to creatinine ratio of 10.0 mg/g. In additive genetic models adjusting for age, sex, ancestry, scanner, intracranial volume, body mass index, hemoglobin A1c, statins, nephropathy, smoking, hypertension, and cardiovascular disease, APOL1 renal-risk-variants were positively associated with gray matter volume (β = 3.4 × 10(-3)) and negatively associated with white matter lesion volume (β = -0.303) (an indicator of cerebral small vessel disease) and cerebrospinal fluid volume (β= -30707) (all significant), but not with white matter volume or cognitive function. Significant associations corresponding to adjusted effect sizes (β/SE) were observed with gray matter volume (0.16) and white matter lesion volume (-0.208), but not with cerebrospinal fluid volume (-0.251). Meta-analysis results with SPRINT Memory and Cognition in Decreased Hypertension (MIND) participants who had cerebral MRI were confirmatory. Thus, APOL1 renal-risk-variants are associated with larger gray matter volume and lower white matter lesion volume suggesting lower intracranial small vessel disease. Copyright © 2016 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.

  9. Comparing Volumes of Prisms and Pyramids

    ERIC Educational Resources Information Center

    Vinogradova, Natalya

    2012-01-01

    Students' experience in using formulas for volumes is often limited to substituting numbers into given formulas. An activity presented in this article may help students make connections between the formulas for volumes of prisms and volumes of pyramids. In addition, some interesting facts from number theory arise, demonstrating strong connections…

  10. Memory performance, global cerebral volumes and hippocampal subfield volumes in long-term survivors of Out-of-Hospital Cardiac Arrest.

    PubMed

    Ørbo, Marte C; Vangberg, Torgil R; Tande, Pål M; Anke, Audny; Aslaksen, Per M

    2018-05-01

    We explored the associations between global brain volumes, hippocampal subfield volumes and verbal memory performance in long-term survivors of out-of-hospital cardiac arrest (OHCA). Three months after OHCA, survivors and healthy, age-matched controls were assessed with cerebral MRI and the California Verbal Learning Test-II (CVLT-II). Volumetric brain segmentation was performed automatically by FreeSurfer. Twenty-six OHCA survivors who were living independently in regular homes at the time of assessment and 19 controls participated in the study. Thirteen of the survivors had been conscious upon arrival to the emergency department. The other 13 survivors had 0.5-7 days of inpatient coma before recovery. Memory was poorer in the OHCA group that had been comatose beyond initial hospital admission compared to both other groups. Total cortical volumes, total hippocampus volumes and several hippocampal subfield volumes were significantly smaller in the OHCA group comatose beyond initial hospital admission compared to controls. No significant differences between the OHCA group conscious upon emergency department arrival and the other two groups were found for brain volumes. No significant differences were observed between any groups for white matter or total subcortical volumes. In OHCA survivors with recovery from inpatient coma, the various CVLT-II trials were significantly, but differentially, correlated to total gray matter volume, cortical volume and the hippocampal subfield subiculum. In this small, single-site study, both hippocampal volume and cortical volume were smaller in good outcome OHCA survivors 3 months after resuscitation in comparison to healthy controls. Smaller cerebral volumes were correlated with poorer memory performance. Copyright © 2018 Elsevier B.V. All rights reserved.

  11. The physics of volume rendering

    NASA Astrophysics Data System (ADS)

    Peters, Thomas

    2014-11-01

    Radiation transfer is an important topic in several physical disciplines, probably most prominently in astrophysics. Computer scientists use radiation transfer, among other things, for the visualization of complex data sets with direct volume rendering. In this article, I point out the connection between physical radiation transfer and volume rendering, and I describe an implementation of direct volume rendering in the astrophysical radiation transfer code RADMC-3D. I show examples for the use of this module on analytical models and simulation data.

  12. Delivered volumes of enteral nutrition exceed prescribed volumes.

    PubMed

    Walker, Renee Nichole; Utech, Anne; Velez, Maria Eugenia; Schwartz, Katie

    2014-10-01

    Enteral nutrition (EN) provisions are typically calculated based on a 24-hour infusion period. However, feedings are often interrupted for daily activities, procedures, or gastrointestinal intolerance. The study's objective was to determine the delivered EN quantities provided to stable hospitalized patients, using cellular time and measured volumes to verify our EN calculation adjustment. A supply of consecutively numbered ready-to-hang (RTH) EN product was delivered to the bedside of 26 inpatients with established EN tolerance at goal rates on various types of nursing units. The dietitian weighed the volume remaining in the infusing product and recorded the measurement time. On the following days, the dietitian continued to weigh the infusing RTH product and the empty RTH bottles saved by nursing. The primary outcome was the difference between the prescribed and delivered EN provisions, which was calculated with a paired t test. Patients received significantly more calories in the delivered enteral feeding (mean [SD], 1678 [385] kcal) than prescribed calories in the EN order (1489 [246 kcal]; t = 3.736, P = .001), adjusting for observed time. No significant differences were found between nursing units, product, and rate. EN delivered may actually exceed ordered amounts by 5%–21% (mean, 12%) with feeding pump inaccuracy as the primary contributing factor. This differs from what others have found. Our findings support using a volume-based ordering system vs a rate-based ordering system for more accurate EN delivery.

  13. O*NET Final Technical Report. Volume I [and] Volume II [and] Volume III.

    ERIC Educational Resources Information Center

    Peterson, Norman G.; Mumford, Michael D.; Borman, Walter C.; Jeanneret, P. Richard; Fleishman, Edwin A.; Levin, Kerry Y.

    This document contains the three volumes of the technical report for development of the prototype of the Occupational Information Network (O*NET), which is intended to replace the "Dictionary of Occupational Titles.""General Introduction" (Norman G. Peterson) presents an overview of O*NET's purpose, content, and structure.…

  14. High volume data storage architecture analysis

    NASA Technical Reports Server (NTRS)

    Malik, James M.

    1990-01-01

    A High Volume Data Storage Architecture Analysis was conducted. The results, presented in this report, will be applied to problems of high volume data requirements such as those anticipated for the Space Station Control Center. High volume data storage systems at several different sites were analyzed for archive capacity, storage hierarchy and migration philosophy, and retrieval capabilities. Proposed architectures were solicited from the sites selected for in-depth analysis. Model architectures for a hypothetical data archiving system, for a high speed file server, and for high volume data storage are attached.

  15. VOLUMNECT: measuring volumes with Kinect

    NASA Astrophysics Data System (ADS)

    Quintino Ferreira, Beatriz; Griné, Miguel; Gameiro, Duarte; Costeira, João. Paulo; Sousa Santos, Beatriz

    2014-03-01

    This article presents a solution to volume measurement object packing using 3D cameras (such as the Microsoft KinectTM). We target application scenarios, such as warehouses or distribution and logistics companies, where it is important to promptly compute package volumes, yet high accuracy is not pivotal. Our application auto- matically detects cuboid objects using the depth camera data and computes their volume and sorting it allowing space optimization. The proposed methodology applies to a point cloud simple computer vision and image processing methods, as connected components, morphological operations and Harris corner detector, producing encouraging results, namely an accuracy in volume measurement of 8mm. Aspects that can be further improved are identified; nevertheless, the current solution is already promising turning out to be cost effective for the envisaged scenarios.

  16. Development of limb volume measuring system

    NASA Technical Reports Server (NTRS)

    Bhagat, P. K.; Kadaba, P. K.

    1983-01-01

    The mechanisms underlying the reductions in orthostatic tolerance associated with weightlessness are not well established. Contradictory results from measurements of leg volume changes suggest that altered venomotor tone and reduced blood flow may not be the only contributors to orthostatic intolerance. It is felt that a more accurate limb volume system which is insensitive to environmental factors will aid in better quantification of the hemodynamics of the leg. Of the varous limb volume techniques presently available, the ultrasonic limb volume system has proven to be the best choice. The system as described herein is free from environmental effects, safe, simple to operate and causes negligible radio frequency interference problems. The segmental ultrasonic ultrasonic plethysmograph is expected to provide a better measurement of limb volume change since it is based on cross-sectional area measurements.

  17. A new electric method for non-invasive continuous monitoring of stroke volume and ventricular volume-time curves

    PubMed Central

    2012-01-01

    Background In this paper a new non-invasive, operator-free, continuous ventricular stroke volume monitoring device (Hemodynamic Cardiac Profiler, HCP) is presented, that measures the average stroke volume (SV) for each period of 20 seconds, as well as ventricular volume-time curves for each cardiac cycle, using a new electric method (Ventricular Field Recognition) with six independent electrode pairs distributed over the frontal thoracic skin. In contrast to existing non-invasive electric methods, our method does not use the algorithms of impedance or bioreactance cardiography. Instead, our method is based on specific 2D spatial patterns on the thoracic skin, representing the distribution, over the thorax, of changes in the applied current field caused by cardiac volume changes during the cardiac cycle. Since total heart volume variation during the cardiac cycle is a poor indicator for ventricular stroke volume, our HCP separates atrial filling effects from ventricular filling effects, and retrieves the volume changes of only the ventricles. Methods ex-vivo experiments on a post-mortem human heart have been performed to measure the effects of increasing the blood volume inside the ventricles in isolation, leaving the atrial volume invariant (which can not be done in-vivo). These effects have been measured as a specific 2D pattern of voltage changes on the thoracic skin. Furthermore, a working prototype of the HCP has been developed that uses these ex-vivo results in an algorithm to decompose voltage changes, that were measured in-vivo by the HCP on the thoracic skin of a human volunteer, into an atrial component and a ventricular component, in almost real-time (with a delay of maximally 39 seconds). The HCP prototype has been tested in-vivo on 7 human volunteers, using G-suit inflation and deflation to provoke stroke volume changes, and LVot Doppler as a reference technique. Results The ex-vivo measurements showed that ventricular filling caused a pattern over the

  18. Site Environmental Report for 2009, Volume 2

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Xu, Suying

    2010-08-19

    Volume II of the Site Environmental Report for 2009 is provided by Ernest Orlando Lawrence Berkeley National Laboratory as a supplemental appendix to Volume I, which contains the body of the report. Volume II contains the environmental monitoring and sampling data used to generate summary results of routine and nonroutine sampling at the Laboratory, except for groundwater sampling data, which may be found in the reports referred to in Chapter 4 of Volume I. The results from sample collections are more comprehensive in Volume II than in Volume I: for completeness, all results from sample collections that began or endedmore » in calendar year (CY) 2009 are included in this volume. However, the samples representing CY 2008 data have not been used in the summary results that are reported in Volume I. (For example, although ambient air samples collected on January 6, 2009, are presented in Volume II, they represent December 2008 data and are not included in Table 4-2 in Volume I.) When appropriate, sampling results are reported in both conventional and International System (SI) units. For some results, the rounding procedure used in data reporting may result in apparent differences between the numbers reported in SI and conventional units. (For example, stack air tritium results reported as < 1.5 Bq/m3 are shown variously as < 39 and < 41 pCi/m3. Both of these results are rounded correctly to two significant digits.)« less

  19. Today's Delinquent. Volumes 1 and 2.

    ERIC Educational Resources Information Center

    Hurst, Hunter, Ed.; And Others

    1983-01-01

    This document contains the first two volumes of "Today's Delinquent," an annual publication of the National Center for Juvenile Justice. The primary focus of both volumes is serious crime by juveniles. Articles in volume one include: (1) "Violent Juvenile Crime: The Problem in Perspective" (Howard N. Snyder); (2) "Canon to the Left, Canon to the…

  20. Renewable Electricity Futures Study - Volume One

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hand, Maureen; Mai, Treui; Baldwin, Sam

    Renewable Electricity Futures Study - Volume One. This is part of a series of four volumes describing exploring a high-penetration renewable electricity future for the United States of America. This data set is provides data for the entire volume one document and includes all data for the charts and graphs included in the document.

  1. Bilingual Preschools. Volume 2: Best Practices

    ERIC Educational Resources Information Center

    Kersten, Kristin, Ed.; Rohde, Andreas, Ed.; Schelletter, Christina, Ed.; Steinlen, Anja K., Ed.

    2010-01-01

    Drawing on data from eleven preschools in four European countries (Germany, Belgium, Sweden, and the UK), this edited volume explores the progress of preschool children learning English over a period of two years. This edited volume (Volume II) gives details on best practices in bilingual preschools as well as background and training on topics…

  2. Modeling measured glottal volume velocity waveforms.

    PubMed

    Verneuil, Andrew; Berry, David A; Kreiman, Jody; Gerratt, Bruce R; Ye, Ming; Berke, Gerald S

    2003-02-01

    The source-filter theory of speech production describes a glottal energy source (volume velocity waveform) that is filtered by the vocal tract and radiates from the mouth as phonation. The characteristics of the volume velocity waveform, the source that drives phonation, have been estimated, but never directly measured at the glottis. To accomplish this measurement, constant temperature anemometer probes were used in an in vivo canine constant pressure model of phonation. A 3-probe array was positioned supraglottically, and an endoscopic camera was positioned subglottically. Simultaneous recordings of airflow velocity (using anemometry) and glottal area (using stroboscopy) were made in 3 animals. Glottal airflow velocities and areas were combined to produce direct measurements of glottal volume velocity waveforms. The anterior and middle parts of the glottis contributed significantly to the volume velocity waveform, with less contribution from the posterior part of the glottis. The measured volume velocity waveforms were successfully fitted to a well-known laryngeal airflow model. A noninvasive measured volume velocity waveform holds promise for future clinical use.

  3. Mortality differences by surgical volume among patients with stomach cancer: a threshold for a favorable volume-outcome relationship.

    PubMed

    Choi, Hyeok; Yang, Seong-Yoon; Cho, Hee-Seung; Kim, Woorim; Park, Eun-Cheol; Han, Kyu-Tae

    2017-07-17

    Many studies have assessed the volume-outcome relationship in cancer patients, but most focused on better outcomes in higher volume groups rather than identifying a specific threshold that could assist in clinical decision-making for achieving the best outcomes. The current study suggests an optimal volume for achieving good outcome, as an extension of previous studies on the volume-outcome relationship in stomach cancer patients. We used National Health Insurance Service (NHIS) Sampling Cohort data during 2004-2013, comprising healthcare claims for 2550 patients with newly diagnosed stomach cancer. We conducted survival analyses adopting the Cox proportional hazard model to investigate the association of three threshold values for surgical volume of stomach cancer patients for cancer-specific mortality using the Youden index. Overall, 17.10% of patients died due to cancer during the study period. The risk of mortality among patients who received surgical treatment gradually decreased with increasing surgical volume at the hospital, while the risk of mortality increased again in "high" surgical volume hospitals, resulting in a j-shaped curve (mid-low = hazard ratio (HR) 0.773, 95% confidence interval (CI) 0.608-0.983; mid-high = HR 0.541, 95% CI 0.372-0.788; high = HR 0.659, 95% CI 0.473-0.917; ref = low). These associations were especially significant in regions with unsubstantial surgical volumes and less severe cases. The optimal surgical volume threshold was about 727.3 surgical cases for stomach cancer per hospital over the 1-year study period in South Korea. However, such positive effects decreased after exceeding a certain volume of surgeries.

  4. CT-based renal volume and graft function after living-donor kidney transplantation: Is there a volume threshold to avoid?

    PubMed

    Dias, Jorge; Malheiro, Jorge; Almeida, Manuela; Dias, Leonídio; Silva-Ramos, Miguel; Martins, La Salete; Xambre, Luís; Castro-Henriques, António

    2015-05-01

    Donated kidney volume influences post-transplant outcomes and graft survival. We evaluated the relationship between living-donor kidney volume and recipient graft function at 12 months post-transplantation, exploring a volume threshold for a suboptimal graft function, and compared two different formulas of volume estimation. A retrospective analysis of 82 pairs of living-donor kidney transplants was conducted. Donor renal volumes were estimated from computerized tomography scans using the ellipsoid formula and the voxel counting technique. Linear and restricted cubic regression spline was used to analyze the association of volume with graft function. Additionally, we determined the correlation between the two volume estimation formulas and established a correction factor for the ellipsoid formula. Renal volume (adjusted to recipient BSA) had the strongest independent effect (B = 1.65 per 10 ml/m(2) increase, p value <0.001) on graft function at 12 months. The eGFR at 12 months was 52.5, 63.6 and 67.6 ml/min/1.73 m(2) for the low, medium and high volume ratio terciles, respectively (p value <0.001). The odds of a GFR <50 ml/min became significantly reduced with volumes above 145 cc/1.73 m(2). A strong positive correlation between the two formulas was identified (R(2) = 0.705), but the optimal correction factor for our cohort was 0.566. In a Caucasian population, higher donor kidney volumes estimated from preoperative CT scans are associated with higher recipient eGFRs at 12 months after live-donor transplantation. Using this criterion, transplant teams can potentially improve selection of living donors if multiple donors are available. However, the need for precise estimation of donor kidney volumes should not be overlooked.

  5. Maximal volume behind horizons without curvature singularity

    NASA Astrophysics Data System (ADS)

    Wang, Shao-Jun; Guo, Xin-Xuan; Wang, Towe

    2018-01-01

    The black hole information paradox is related to the area of event horizon, and potentially to the volume and singularity behind it. One example is the complexity/volume duality conjectured by Stanford and Susskind. Accepting the proposal of Christodoulou and Rovelli, we calculate the maximal volume inside regular black holes, which are free of curvature singularity, in asymptotically flat and anti-de Sitter spacetimes respectively. The complexity/volume duality is then applied to anti-de Sitter regular black holes. We also present an analytical expression for the maximal volume outside the de Sitter horizon.

  6. Survival Estimates for the Passage of Spring-Migrating Juvenile Salmonids through Snake and Columbia River Dams and Reservoirs, 2003-2004 Annual Report.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Smith, Steven G.; Muir, William D.; Zabel, Richard W.

    2004-01-01

    For juvenile chinook salmon Oncorhynchus tshawytscha, sockeye salmon O. nerka, and steelhead O. mykiss that migrate through reservoirs, hydroelectric projects, and free-flowing sections of the Snake and Columbia Rivers, survival estimates are essential to develop effective strategies for recovering depressed stocks. Many management strategies were based on estimates of system survival (Raymond 1979; Sims and Ossiander 1981) derived in a river system considerably different from today's (Williams and Matthews 1995; Williams et al. 2001). Knowledge of the magnitude, locations, and causes of smolt mortality under present passage conditions, and under conditions projected for the future, are necessary to develop strategiesmore » that will optimize smolt survival during migration. From 1993 through 2002, the National Marine Fisheries Service (NMFS) and the University of Washington (UW) demonstrated the feasibility of using three statistical models to estimate survival of PIT-tagged (Prentice et al. 1990a) juvenile salmonids passing through Snake River dams and reservoirs (Iwamoto et al. 1994; Muir et al. 1995, 1996, 2001a, 2003; Smith et al. 1998, 2000a,b; Hockersmith et al. 1999; Zabel et al. 2001, 2002). Evaluation of assumptions for these models indicated that all were generally satisfied, and accurate and precise survival estimates were obtained. In 2003, NMFS and UW completed the eleventh year of the study. Flow levels during the early portion of the 2003 spring migration were similar to 2002, and only slightly higher than in the drought conditions during 2001. However, flow levels were much greater during the later part of the migration in 2003. Spill levels were similar to 2002, much higher than in 2001. Research objectives were to: (1) estimate reach survival and travel time in the Snake and Columbia Rivers throughout the yearling chinook salmon and steelhead migrations; (2) evaluate relationships between survival estimates and migration conditions; and (3

  7. 21 CFR 862.1130 - Blood volume test system.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Blood volume test system. 862.1130 Section 862....1130 Blood volume test system. (a) Identification. A blood volume test system is a device intended to measure the circulating blood volume. Blood volume measurements are used in the diagnosis and treatment of...

  8. Learn Japanese--Elementary School Text, Volume III.

    ERIC Educational Resources Information Center

    Sato, Yaeko; And Others

    This volume is the teacher's text for the first semester program on level two (fourth grade). See AL 001 718 for Volume I and ED 019 666 for Volume II. Text materials for the second level continue to introduce new structures systematically, according to the pupils' interest, ability, and rate of learning. Dialogs for level two, Volumes III and IV,…

  9. Control volume based hydrocephalus research

    NASA Astrophysics Data System (ADS)

    Cohen, Benjamin; Voorhees, Abram; Wei, Timothy

    2008-11-01

    Hydrocephalus is a disease involving excess amounts of cerebral spinal fluid (CSF) in the brain. Recent research has shown correlations to pulsatility of blood flow through the brain. However, the problem to date has presented as too complex for much more than statistical analysis and understanding. This talk will highlight progress on developing a fundamental control volume approach to studying hydrocephalus. The specific goals are to select physiologically control volume(s), develop conservation equations along with the experimental capabilities to accurately quantify terms in those equations. To this end, an in vitro phantom is used as a simplified model of the human brain. The phantom's design consists of a rigid container filled with a compressible gel. The gel has a hollow spherical cavity representing a ventricle and a cylindrical passage representing the aquaducts. A computer controlled piston pump supplies pulsatile volume fluctuations into and out of the flow phantom. MRI is used to measure fluid velocity, and volume change as functions of time. Independent pressure measurements and flow rate measurements are used to calibrate the MRI data. These data are used as a framework for future work with live patients.

  10. Adult chinook salmon passage at Little Goose Dam in relation to spill operations

    USGS Publications Warehouse

    Jepson, M.A.; Caudill , C.C.; Clabough, T.S.; Peery, C.A.; Beeman, J.W.; Fielding, S.

    2009-01-01

    Spill patterns at Little Goose Dam in 2007 were modified in anticipation of a spillway weir installation intended to improve downstream passage of juvenile salmonids. However, in spill pattern was associated with reduced daily counts of adult salmon passing the dam. Consequently, the behaviors and upstream passage times of radio-tagged adult spring–summer Chinook salmon were evaluated in response to three spillway discharge patterns at Little Goose Dam during 2008. Simultaneously, tailrace conditions were characterized by monitoring the downstream paths of GPS-equipped drogues. Two of the spill treatments (i.e., Bulk and Alternate) were variations of patterns thought to mimic those produced if a spillway weir was installed. The third treatment (Uniform) was characterized by spilling similar volumes of water through most spillbays.

  11. Factors influencing the measurement of closing volume.

    PubMed

    Make, B; Lapp, N L

    1975-06-01

    The various factors influencing closing volume were studied by performing the single-breath N2 test on 9 healthy nonsmokers. Time of day, day of the week, and preceding volume history had no effect on either closing volume or alveolar plateau. Slow inspiratory flow resulted in larger ratio of closing volume to vital capacity, ratio of closing capacity to total lung capacity, and change in N2 concentration than fast inspiratory flow. Voluntary regulation of the expiratory flow resulted in smaller ratios of closing volume to vital capacity and closing capacity to total lung capacity than when flow was regulated by a resistance. Prolonged breath holding of the inspired O2 led to larger ratio of closing volume to vital capacity and ratio of closing capacity to total lung capacity. To obtain uniform, comparable closing volumes, it is suggested that the subject inspire slowly, control expiratory flow (preferably voluntarily), and not pause between inspiration and expiration.

  12. China’s Rise to Power: Revitalizing the Ties with the Republic of the Philippines to meet 21st Century Security Interests

    DTIC Science & Technology

    2009-01-01

    a potential military conflict against China in the future. 20 1 Russell Ong , China’s Security Interests in the 21’t Century. (New York, NY: Routledge...Conflictfrom 1500 to 2000 (New York: Random House, 1987), xxii. 11 Ong , China’s Security Interests in the 21st Centwy, 124. 12 Qiao Liang, and Wang Xiangsui...Strategies and their Implicationsfor the United States. xiv. 16 Ibid. xv. 17 Ibid. 18 Ong , China’s Security Interests in the 21’t Centwy, 124. 19 Ibid. 20

  13. In search of the dimensions of an incandescent light bulb filament

    NASA Astrophysics Data System (ADS)

    Ladino, Luis A.; Rondón, Hermilda S.

    2018-05-01

    The purpose of this paper is to present and discuss an alternative solution to an experimental problem given to high school students in the XXII Ibero-American Physics Olympiad held by Colombia this year. From the measurements of electric current and potential difference across a small tungsten filament lamp students should find the dimensions of its filament. The results obtained are compared with the ones measured directly. This challenging and low-cost experiment can be easily implemented and carried out in any introductory physics laboratory courses.

  14. The volume of the human knee joint.

    PubMed

    Matziolis, Georg; Roehner, Eric; Windisch, Christoph; Wagner, Andreas

    2015-10-01

    Despite its clinical relevance, particularly in septic knee surgery, the volume of the human knee joint has not been established to date. Therefore, the objective of this study was to determine knee joint volume and whether or not it is dependent on sex or body height. Sixty-one consecutive patients (joints) who were due to undergo endoprosthetic joint replacement were enrolled in this prospective study. During the operation, the joint volume was determined by injecting saline solution until a pressure of 200 mmHg was achieved in the joint. The average volume of all knee joints was 131 ± 53 (40-290) ml. The volume was not found to be dependent on sex, but it was dependent on the patients' height (R = 0.312, p = 0.014). This enabled an estimation of the joint volume according to V = 1.6 height - 135. The considerable inter-individual variance of the knee joint volume would suggest that it should be determined or at least estimated according to body height if the joint volume has consequences for the diagnostics or therapy of knee disorders.

  15. Converting international ¼ inch tree volume to Doyle

    Treesearch

    Aaron Holley; John R. Brooks; Stuart A. Moss

    2014-01-01

    An equation for converting Mesavage and Girard's International ¼ inch tree volumes to the Doyle log rule is presented as a function of tree diameter. Volume error for trees having less than four logs exhibited volume prediction errors within a range of ±10 board feet. In addition, volume prediction error as a percent of actual Doyle tree volume...

  16. Delivery of tidal volume from four anaesthesia ventilators during volume-controlled ventilation: a bench study.

    PubMed

    Wallon, G; Bonnet, A; Guérin, C

    2013-06-01

    Tidal volume (V(T)) must be accurately delivered by anaesthesia ventilators in the volume-controlled ventilation mode in order for lung protective ventilation to be effective. However, the impact of fresh gas flow (FGF) and lung mechanics on delivery of V(T) by the newest anaesthesia ventilators has not been reported. We measured delivered V(T) (V(TI)) from four anaesthesia ventilators (Aisys™, Flow-i™, Primus™, and Zeus™) on a pneumatic test lung set with three combinations of lung compliance (C, ml cm H2O(-1)) and resistance (R, cm H2O litre(-1) s(-2)): C60R5, C30R5, C60R20. For each CR, three FGF rates (0.5, 3, 10 litre min(-1)) were investigated at three set V(T)s (300, 500, 800 ml) and two values of PEEP (0 and 10 cm H2O). The volume error = [(V(TI) - V(Tset))/V(Tset)] ×100 was computed in body temperature and pressure-saturated conditions and compared using analysis of variance. For each CR and each set V(T), the absolute value of the volume error significantly declined from Aisys™ to Flow-i™, Zeus™, and Primus™. For C60R5, these values were 12.5% for Aisys™, 5% for Flow-i™ and Zeus™, and 0% for Primus™. With an increase in FGF, absolute values of the volume error increased only for Aisys™ and Zeus™. However, in C30R5, the volume error was minimal at mid-FGF for Aisys™. The results were similar at PEEP 10 cm H2O. Under experimental conditions, the volume error differed significantly between the four new anaesthesia ventilators tested and was influenced by FGF, although this effect may not be clinically relevant.

  17. Two Cathelicidin Genes Are Present in both Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar)

    PubMed Central

    Chang, Chin-I; Zhang, Yong-An; Zou, Jun; Nie, Pin; Secombes, Christopher J.

    2006-01-01

    Further to the previous finding of the rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two in Atlantic salmon, named asCATH_1 and asCATH_2, and one in rainbow trout, named rtCATH_2. All the three new salmonid cathelicidin genes share the common characteristics of mammalian cathelicidin genes, such as consisting of four exons and possessing a highly conserved preproregion and four invariant cysteines clustered in the C-terminal region of the cathelin-like domain. The asCATH_1 gene is homologous to the rainbow trout rtCATH_1 gene, in that it possesses three repeat motifs of TGGGGGTGGC in exon IV and two cysteine residues in the predicted mature peptide, while the asCATH_2 gene and rtCATH_2 gene are homologues of each other, with 96% nucleotide identity. Salmonid cathelicidins possess the same elastase-sensitive residue, threonine, as hagfish cathelicidins and the rabbit CAP18 molecule. The cleavage site of the four salmonid cathelicidins is within a conserved amino acid motif of QKIRTRR, which is at the beginning of the sequence encoded by exon IV. Two 36-residue peptides corresponding to the core part of rtCATH_1 and rtCATH_2 were chemically synthesized and shown to exhibit potent antimicrobial activity. rtCATH_2 was expressed constitutively in gill, head kidney, intestine, skin and spleen, while the expression of rtCATH_1 was inducible in gill, head kidney, and spleen after bacterial challenge. Four cathelicidin genes have now been characterized in salmonids and two were identified in hagfish, confirming that cathelicidin genes evolved early and are likely present in all vertebrates. PMID:16377685

  18. Membranes with artificial free-volume for biofuel production

    NASA Astrophysics Data System (ADS)

    Petzetakis, Nikos; Doherty, Cara M.; Thornton, Aaron W.; Chen, X. Chelsea; Cotanda, Pepa; Hill, Anita J.; Balsara, Nitash P.

    2015-06-01

    Free-volume of polymers governs transport of penetrants through polymeric films. Control over free-volume is thus important for the development of better membranes for a wide variety of applications such as gas separations, pharmaceutical purifications and energy storage. To date, methodologies used to create materials with different amounts of free-volume are based primarily on chemical synthesis of new polymers. Here we report a simple methodology for generating free-volume based on the self-assembly of polyethylene-b-polydimethylsiloxane-b-polyethylene triblock copolymers. We have used this method to fabricate a series of membranes with identical compositions but with different amounts of free-volume. We use the term artificial free-volume to refer to the additional free-volume created by self-assembly. The effect of artificial free-volume on selective transport through the membranes was tested using butanol/water and ethanol/water mixtures due to their importance in biofuel production. We found that the introduction of artificial free-volume improves both alcohol permeability and selectivity.

  19. Localized Arm Volume Index: A New Method for Body Type-Corrected Evaluation of Localized Arm Lymphedematous Volume Change.

    PubMed

    Yamamoto, Takumi; Yamamoto, Nana; Yoshimatsu, Hidehiko

    2017-10-01

    Volume measurement is a common evaluation for upper extremity lymphedema. However, volume comparison between different patients with different body types may be inappropriate, and it is difficult to evaluate localized limb volume change using arm volume. Localized arm volumes (Vk, k = 1-5) and localized arm volume indices (LAVIk) at 5 points (1, upper arm; 2, elbow; 3, forearm; 4, wrist; 5, hand) of 106 arms of 53 examinees with no arm edema were calculated based on physical measurements (arm circumferences and lengths and body mass index [BMI]). Interrater and intrarater reliabilities of LAVIk were assessed, and Vk and LAVIk were compared between lower BMI (BMI, <22 kg/m) group and higher BMI (BMI, ≥22 kg/m) group. Interrater and intrarater reliabilities of LAVIk were all high (all, r > 0.98). Between lower and higher BMI groups, significant differences were observed in all Vk (V1 [P = 6.8 × 10], V2 [P = 3.1 × 10], V3 [P = 1.1 × 10], V4 [P = 8.3 × 10], and V5 [P = 3.0 × 10]). Regarding localized arm volume index (LAVI) between groups, significant differences were seen in LAVI1 (P = 9.7 × 10) and LAVI5 (P = 1.2 × 10); there was no significant difference in LAVI2 (P = 0.60), LAVI3 (P = 0.61), or LAVI4 (P = 0.22). Localized arm volume index is a convenient and highly reproducible method for evaluation of localized arm volume change, which is less affected by body physique compared with arm volumetry.

  20. The relationships between breast volume, breast dense volume and volumetric breast density with body mass index, body fat mass and ethnicity

    NASA Astrophysics Data System (ADS)

    Zakariyah, N.; Pathy, N. B.; Taib, N. A. M.; Rahmat, K.; Judy, C. W.; Fadzil, F.; Lau, S.; Ng, K. H.

    2016-03-01

    It has been shown that breast density and obesity are related to breast cancer risk. The aim of this study is to investigate the relationships of breast volume, breast dense volume and volumetric breast density (VBD) with body mass index (BMI) and body fat mass (BFM) for the three ethnic groups (Chinese, Malay and Indian) in Malaysia. We collected raw digital mammograms from 2450 women acquired on three digital mammography systems. The mammograms were analysed using Volpara software to obtain breast volume, breast dense volume and VBD. Body weight, BMI and BFM of the women were measured using a body composition analyser. Multivariable logistic regression was used to determine the independent predictors of increased overall breast volume, breast dense volume and VBD. Indians have highest breast volume and breast dense volume followed by Malays and Chinese. While Chinese are highest in VBD, followed by Malay and Indian. Multivariable analysis showed that increasing BMI and BFM were independent predictors of increased overall breast volume and dense volume. Moreover, BMI and BFM were independently and inversely related to VBD.