Design review and analysis for a Pratt and Whitney fluid-film bearing and seal testing rig

NASA Technical Reports Server (NTRS)

Childs, Dara W.

1994-01-01

A design review has been completed for a Pratt and Whitney (P&W)-designed fluid-film bearing and annular-seal test rig to be manufactured and installed at George C. Marshall Space Flight Center (MSFC). Issues covered in this study include: (1) the capacity requirements of the drive unit; (2) the capacity and configuration of the static loading system; (3) the capacity and configuration of the dynamic excitation system; (4) the capacity, configuration, and rotordynamic stability of a test bearing, support bearings, and shaft; and (5) the characteristics and configuration of the measurement transducers and data channels.

The influence of shrouded stator cavity flows on multistage compressor performance

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wellborn, S.R.; Okiishi, T.H.

1999-07-01

Experiments were performed on a low-speed multistage axial-flow compressor to assess the effects of shrouded stator cavity flows on aerodynamic performance. Five configurations, which involved systematic changes in seal-tooth leakage rates and/or elimination of the shrouded stator cavities, were tested. Rig data indicate increasing seal-tooth leakage substantially degraded compressor performance. For every 1 percent increase in seal-tooth clearance-to-span ratio, the decrease in pressure rise was 3 percent and the reduction in efficiency was 1 point. These observed performance penalties are comparable to those commonly reported for rotor and cantilevered stator tip clearance variations. The performance degradation observed with increased leakagemore » was brought about in two distinct ways. First, increasing seal-tooth leakage directly spoiled the near-hub performance of the stator row in which leakage occurred. Second, the altered stator exit flow conditions, caused by increased leakage, impaired the performance of the next downstream stage by decreasing the work input of the rotor and increasing total pressure loss of the stator. These trends caused the performance of downstream stages to deteriorate progressively. Numerical simulations of the test rig stator flow field were also conducted to help resolve important fluid mechanic details associated with the interaction between the primary and cavity flows. Simulation results show that fluid originating in the upstream cavity collected on the stator suction surface when the cavity tangential momentum was low and on the pressure side when it was high. The convection of cavity fluid to the suction surface was a mechanism that reduced stator performance when leakage increased.« less

Labyrinth Seal Analysis. Volume 3. Analytical and Experimental Development of a Design Model for Labyrinth Seals

DTIC Science & Technology

1986-01-01

the information that has been determined experimentally. The Labyrinth Seal Analysis program was, therefore, directed to the develop - ment of an...labyrinth seal performance, the program included the development of an improved empirical design model to pro- j. .,’ vide the calculation of the flow... program . * Phase I was directed to the analytical development of both an *analysis* model and an improvwd empirical *design" model. Supporting rig tests

Labyrinth seal testing for lift fan engines

NASA Technical Reports Server (NTRS)

Dobek, L. J.

1973-01-01

An abradable buffered labyrinth seal for the control of turbine gas path leakage in a tip-turbine driven lift fan was designed, tested, and analyzed. The seal configuration was not designed to operate in any specific location but was sized to be evaluated in an existing test rig. The final sealing diameter selected was 28 inches. Results of testing indicate that the flow equations predicted seal air flows consistent with measured values. Excellent sealing characteristics of the abradable coating on the stator land were demonstrated when a substantial seal penetration of .030 inch into the land surface was encountered without appreciable wear on the labyrinth knife edges.

Detailed Validation Assessment of Turbine Stage Disc Cavity Rotating Flows

NASA Astrophysics Data System (ADS)

Kanjiyani, Shezan

The subject of this thesis is concerned with the amount of cooling air assigned to seal high pressure turbine rim cavities which is critical for performance as well as component life. Insufficient air leads to excessive hot annulus gas ingestion and its penetration deep into the cavity compromising disc life. Excessive purge air, adversely affects performance. Experiments on a rotating turbine stage rig which included a rotor-stator forward disc cavity were performed at Arizona State University. The turbine rig has 22 vanes and 28 blades, while the rim cavity is composed of a single-tooth rim lab seal and a rim platform overlap seal. Time-averaged static pressures were measured in the gas path and the cavity, while mainstream gas ingestion into the cavity was determined by measuring the concentration distribution of tracer gas (carbon dioxide). Additionally, particle image velocimetry (PIV) was used to measure fluid velocity inside the rim cavity between the lab seal and the overlap. The data from the experiments were compared to an 360-degree unsteady RANS (URANS) CFD simulations. Although not able to match the time-averaged test data satisfactorily, the CFD simulations brought to light the unsteadiness present in the flow during the experiment which the slower response data did not fully capture. To interrogate the validity of URANS simulations in capturing complex rotating flow physics, the scope of this work also included to validating the CFD tool by comparing its predictions against experimental LDV data in a closed rotor-stator cavity. The enclosed cavity has a stationary shroud, a rotating hub, and mass flow does not enter or exit the system. A full 360 degree numerical simulation was performed comparing Fluent LES, with URANS turbulence models. Results from these investigations point to URANS state of art under-predicting closed cavity tangential velocity by 32% to 43%, and open rim cavity effectiveness by 50% compared to test data. The goal of this thesis is to assess the validity of URANS turbulence models in more complex rotating flows, compare accuracy with LES simulations, suggest CFD settings to better simulate turbine stage mainstream/disc cavity interaction with ingestion, and recommend experimentation techniques.

iLIDS Simulations and Videos for Docking TIM

NASA Technical Reports Server (NTRS)

Lewis, James L.

2010-01-01

The video shows various aspects of the International Low Impact Docking System, including team members, some production, configuration, mated androgynous iLIDS, SCS Lockdown system, thermal analysis, electrical engineering aspects, the iLIDS control box and emulator, radiation testing at BNL, component environmental testing, component vibration testing, 3G processor board delivery system, GTA vibe test, EMA testbed, hook and hook disassembly, flex shaftdrive assembly, GSE cradle MISSE-6 Columbus, MISSE 6 and 7 seal experiments, actuated full scale seal test rig, LIDS on Hubble, dynamics test prep, EDU 54 mass emulation and SCS, load ring characterization, 6DOF proof test, SCS at 6DOF, machining EEMS and inner ring assembly, APAS assembly, inner ring fitting, rotation stand assembly, EEMS mating, and EEMS proof of concept demonstration.

The experimental evaluation and application of high-temperature solid lubricants. Ph.D. Thesis - Case Western Reserve Univ., 1989 Final Report

NASA Technical Reports Server (NTRS)

Dellacorte, Christopher

1990-01-01

A research program is described which developes an understanding of high-temperature solid lubrication and experimental techniques through the development of a composite lubricant coating system. The knowledge gained through this research was then applied to a specific engineering challenge, the tribology of a sliding seal for hypersonic flight vehicles. The solid lubricant coating is a chromium carbide based composite combined with silver, which acts as a low temperature lubricant, and barium fluoride/calcium fluoride eutectic, which acts as a high-temperature lubricant. This composite coating provides good wear resistance and low friction for sliding contacts from room temperature to over 900 C in reducing or oxidative environments. The specific research on this coating included a composition screening using a foil gas bearing test rig and the use of thin silver films to reduce initial wear using a pin-on-disk test rig. The chemical stability of the materials used was also addressed. This research indicated that soft metallic films and materials which become soft at elevated temperatures are potentially good lubricants. The general results from the experiments with the model solid lubricant coating were then applied to a sliding seal design concept. This seal design requires that a braided ceramic fabric slide against a variety of metal counterface materials at temperatures from 25 to 850 C in an oxidative environment. A pin-on-disk tribometer was used to evaluate the tribological properties of these materials and to develop lubrication techniques. The results indicate that these seal materials must be lubricated to prevent wear and reduce friction. Thin films of silver, gold and calcium fluoride provided lubrication to the sliding materials.

A hybrid floating brush seal (HFBS) for improved sealing and wear performance in turbomachinery applications

NASA Astrophysics Data System (ADS)

Lattime, Scott Byran

A conceptually new type of seal has been developed for gas turbine applications which dramatically reduces wear and leakage associated with current labyrinth and brush seal technologies. The Hybrid Floating Brush Seal (HFBS) combines brush seal and film riding face seal technologies to create a hybrid seal that allows both axial and radial excursions of the sealed shaft, while simultaneously eliminating interface surface speeds (friction and heat) between the rotor and the brush material that characterize standard brush seal technology. A simple test rig was designed to evaluate feasibility of the HFBS under relatively low pressures and rotational speeds (50psig, 5krpm). A second test stand was created to study the effects of centrifugal force on bristle deflection. A third test facility was constructed for prototype development and extensive room temperature testing at moderate pressures and fairly high rotational speeds (100psig, 40krpm). This test rig also allowed the evaluation of the HFBS during axial movement of a rotating shaft. An analytical model to predict the effects of centrifugal force on the bristles of a rotating brush seal was developed. Room temperature analysis of the HFBS proved successful for relatively high operating rotational velocities at moderate pressures with very acceptable leakage rates for gas turbine engines. Brush seals were able to track rotor speeds up to 24krpm while maintaining sealing integrity. The HFBS's ability to function under axial shaft displacement and synchronous dynamic radial loading was also proven successful. Hydrodynamic performance of the face seal was proven to provide adequate stiffness and load carrying capacity to keep the brush seal from contacting the face seal at pressure drops across the brush of up to 100psi. Leakage performance over standard brush seal and labyrinth technology was quite dramatic. The HFBS showed its sealing advantage using much higher radial interference between the rotor and the bristle bore over standard brush seal designs. Experimental results of the HFBS showed leakage reductions of 6 to 7 times that of a standard brush seal at the same operating pressure ratios and rotational speed and an order of magnitude less than numerical predictions of a standard labyrinth seal. (Abstract shortened by UMI.)

JT9D ceramic outer air seal system refinement program

NASA Technical Reports Server (NTRS)

Gaffin, W. O.

1982-01-01

The abradability and durability characteristics of the plasma sprayed system were improved by refinement and optimization of the plasma spray process and the metal substrate design. The acceptability of the final seal system for engine testing was demonstrated by an extensive rig test program which included thermal shock tolerance, thermal gradient, thermal cycle, erosion, and abradability tests. An interim seal system design was also subjected to 2500 endurance test cycles in a JT9D-7 engine.

High-Speed, High-Temperature Finger Seal Test Results

NASA Technical Reports Server (NTRS)

Proctor, Margaret P.; Kumar, Arun; Delgado, Irebert R.

2002-01-01

Finger seals have significantly lower leakage rates than conventional labyrinth seals used in gas turbine engines and are expected to decrease specific fuel consumption by over 1 percent and to decrease direct operating cost by over 0.5 percent. Their compliant design accommodates shaft growth and motion due to thermal and dynamic loads with minimal wear. The cost to fabricate these finger seals is estimated to be about half the cost to fabricate brush seals. A finger seal has been tested in NASA's High Temperature, High Speed Turbine Seal Test Rig at operating conditions up to 1200 F, 1200 ft/s, and 75 psid. Static, performance and endurance test results are presented. While seal leakage and wear performance are acceptable, further design improvements are needed to reduce the seal power loss.

Development of helicopter transmission seals, task 2

NASA Technical Reports Server (NTRS)

Hayden, T. S.; Keller, C. H., Jr.

1973-01-01

High speed helicopter transmission seal concepts were designed, fabricated and tested. The concepts were a dual element split ring seal and a circumferential seal. The tests were performed in a rig using an actual input quill assembly. The test conditions were selected to simulate transmission operation and were 230 F oil temperature, and a sliding speed of 9400 ft/min. The split ring seal exhibited gross leakage and was considered unsatisfactory, while the circumferential seal leakage was less than 1 c.c./hour; this leakage is within acceptable limits. The circumferential seal wear was only to .0005 inches during a 100 hour run (40 starts and stops). During a 40 hour contamination test (mesh silica flour) the seal total wear was a maximum of .004 inches. This wear is considered acceptable.

Brush Seals for Improved Steam Turbine Performance

NASA Technical Reports Server (NTRS)

Turnquist, Norman; Chupp, Ray; Baily, Fred; Burnett, Mark; Rivas, Flor; Bowsher, Aaron; Crudgington, Peter

2006-01-01

GE Energy has retrofitted brush seals into more than 19 operating steam turbines. Brush seals offer superior leakage control compared to labyrinth seals, owing to their compliant nature and ability to maintain very tight clearances to the rotating shaft. Seal designs have been established for steam turbines ranging in size from 12 MW to over 1200 MW, including fossil, nuclear, combined-cycle and industrial applications. Steam turbines present unique design challenges that must be addressed to ensure that the potential performance benefits of brush seals are realized. Brush seals can have important effects on the overall turbine system that must be taken into account to assure reliable operation. Subscale rig tests are instrumental to understanding seal behavior under simulated steam-turbine operating conditions, prior to installing brush seals in the field. This presentation discusses the technical challenges of designing brush seals for steam turbines; subscale testing; performance benefits of brush seals; overall system effects; and field applications.

A Hot Dynamic Seal Rig for Measuring Hypersonic Engine Seal Durability and Flow Performance

NASA Technical Reports Server (NTRS)

Miller, Jeffrey H.; Steinetz, Bruce M.; Sirocky, Paul J.; Kren, Lawrence A.

1993-01-01

A test fixture for measuring the dynamic performance of candidate high-temperature engine seal concepts was installed at NASA Lewis Research Center. The test fixture was designed to evaluate seal concepts under development for advanced hypersonic engines, such as those being considered for the National Aerospace Plane (NASP). The fixture can measure dynamic seal leakage performance from room temperature up to 840 C (1550 F) and air pressure differentials up to 690 kPa (100 psi). Performance of the seals can be measured while sealing against flat or distorted walls. In the fixture two seals are preloaded against the sides of a 30 cm (1 ft) long saber that slides transverse to the axis of the seals, simulating the scrubbing motion anticipated in these engines. The capabilities of this test fixture along with preliminary data showing the dependence of seal leakage performance on high temperature cycling are addressed.

A hot dynamic seal rig for measuring hypersonic engine seal durability and flow performance

NASA Technical Reports Server (NTRS)

Miller, Jeffrey H.; Steinetz, Bruce M.; Sirocky, Paul J.; Kren, Lawrence A.

1993-01-01

A test fixture for measuring the dynamic performance of candidate high-temperature engine seal concepts has been installed at NASA Lewis Research Center. The test fixture has been designed to evaluate seal concepts under development for advanced hypersonic engines, such as those being considered for the National Aerospace Plane (NASP). The fixture can measure dynamic seal leakage performance from room temperature up to 840 C (1550 F) and air pressure differentials up to 690 kPa (100 psi). Performance of the seals can be measured while sealing against flat or distorted walls. In the fixture two seals are preloaded against the sides of a 30 cm (1 ft) long saber that slides transverse to the axis of the seals, simulating the scrubbing motion anticipated in these engines. This report covers the capabilities of this test fixture along with preliminary data showing the dependence of seal leakage performance on high temperature cycling.

High Misalignment Carbon Seals for the Fan Drive Gear System Technologies

NASA Technical Reports Server (NTRS)

Shaughnessy, Dennis; Dobek, Lou

2006-01-01

Aircraft engines of the future will require capability bearing compartment seals than found in current engines. Geared systems driving the fan will be subjected to inertia and gyroscopic forces resulting in extremely high angular and radial misalignments. Because of the high misalignment levels, compartment seals capable of accommodating angularities and eccentricities are required. Pratt & Whitney and Stein Seal Company selected the segmented circumferential carbon seal as the best candidate to operate at highly misaligned conditions. Initial seal tests established the misalignment limits of the current technology circumferential seal. From these results a more compliant seal configuration was conceived, designed, fabricated, and tested. Further improvements to the design are underway and plans are to conduct a durability test of the next phase configuration. A technical approach is presented, including design modification to a "baseline"seal, carbon grade selection, test rig configuration, test plan and results of analysis of seal testing.

2003 NASA Seal/Secondary Air System Workshop. Volume 1

NASA Technical Reports Server (NTRS)

Steinetz, Bruce M. (Editor); Hendricks, Robert C. (Editor)

2004-01-01

The following reports were included in the 2003 NASA Seal/Secondary Air System Workshop:Low Emissions Alternative Power (LEAP); Overview of NASA Glenn Seal Developments; NASA Ultra Efficient Engine Technology Project Overview; Development of Higher Temperature Abradable Seals for Industrial Gas Turbines; High Misalignment Carbon Seals for the Fan Drive Gear System Technologies; Compliant Foil Seal Investigations; Test Rig for Evaluating Active Turbine Blade Tip Clearance Control Concepts; Controls Considerations for Turbine Active Clearance Control; Non-Contacting Finger Seal Developments and Design Considerations; Effect of Flow-Induced Radial Load on Brush Seal/Rotor Contact Mechanics; Seal Developments at Flowserve Corporation; Investigations of High Pressure Acoustic Waves in Resonators With Seal-Like Features; Numerical Investigations of High Pressure Acoustic Waves in Resonators; Feltmetal Seal Material Through-Flow; "Bimodal" Nuclear Thermal Rocket (BNTR) Propulsion for Future Human Mars Exploration Missions; High Temperature Propulsion System Structural Seals for Future Space Launch Vehicles; Advanced Control Surface Seal Development for Future Space Vehicles; High Temperature Metallic Seal Development for Aero Propulsion and Gas Turbine Applications; and BrazeFoil Honeycomb.

Development of spiral-groove self-acting seals for helicopter engines

NASA Technical Reports Server (NTRS)

Obrien, M.

1979-01-01

A spiral-groove, self-acting face seal was rig tested at advanced gas turbine operating conditions to determine wear and leakage rates. The spiral-groove, self-acting geometry was located in the rotating seal seat. Seal component wear induced by start-stop operation was measured after subjecting the test seal to 176 start-stop cycles. Wear occurring during normal operation was documented throughout a 75-hour endurance test. Seal air leakage was also measured. During endurance operation, the seal was subjected to operating conditions bounded by the values surface speed - 244 m/s (800 ft/sec), air pressure - 148 N/sq cm abs (215 psia), and air temperature - 622 K (660 F). The post-test condition of the seal components was documented. Wear data is presented in tabular form, while seal air leakage is presented graphically, as a function of pressure and speed.

Hot dynamic test rig for measuring hypersonic engine seal flow and durability

NASA Technical Reports Server (NTRS)

Miller, Jeffrey H.; Steinetz, Bruce M.; Sirocky, Paul J.; Kren, Lawrence A.

1994-01-01

A test fixture for measuring the dynamic performance of candidate high-temperature engine seal concepts was developed. The test fixture was developed to evaluate seal concepts under development for advanced hypersonic engines, such as those being considered for the National Aerospace Plane (NASP). The fixture can measure dynamic seal leakage performance from room temperature up to 840 C and air pressure differentials of to 0.7 MPa. Performance of the seals can be measured while sealing against flat or engine-simulated distorted walls. In the fixture, two seals are preloaded against the sides of a 0.3 m long saber that slides transverse to the axis of the seals, simulating the scrubbing motion anticipated in these engines. The capabilities of this text fixture along with preliminary data showing the dependence of seal leakage performance on high temperature cycling are covered.

Test Rig for Evaluating Active Turbine Blade Tip Clearance Control Concepts

NASA Technical Reports Server (NTRS)

Lattime, Scott B.; Steinetz, Bruce M.; Robbie, Malcolm G.

2003-01-01

Improved blade tip sealing in the high pressure compressor and high pressure turbine can provide dramatic improvements in specific fuel consumption, time-on-wing, compressor stall margin and engine efficiency as well as increased payload and mission range capabilities of both military and commercial gas turbine engines. The preliminary design of a mechanically actuated active clearance control (ACC) system for turbine blade tip clearance management is presented along with the design of a bench top test rig in which the system is to be evaluated. The ACC system utilizes mechanically actuated seal carrier segments and clearance measurement feedback to provide fast and precise active clearance control throughout engine operation. The purpose of this active clearance control system is to improve upon current case cooling methods. These systems have relatively slow response and do not use clearance measurement, thereby forcing cold build clearances to set the minimum clearances at extreme operating conditions (e.g., takeoff, re-burst) and not allowing cruise clearances to be minimized due to the possibility of throttle transients (e.g., step change in altitude). The active turbine blade tip clearance control system design presented herein will be evaluated to ensure that proper response and positional accuracy is achievable under simulated high-pressure turbine conditions. The test rig will simulate proper seal carrier pressure and temperature loading as well as the magnitudes and rates of blade tip clearance changes of an actual gas turbine engine. The results of these evaluations will be presented in future works.

Advanced Gas Turbine (AGT) technology report

NASA Technical Reports Server (NTRS)

1985-01-01

Engine testing, ceramic component fabrication and evaluation, component performance rig testing, and producibility experiments at Pontiac comprised AGT 100 activities of this period, January to December 1984. Two experimental engines were available and allowed the evaluation of eight experimental assemblies. Operating time accumulated was 115 hr of burning and 156 hr total. Total cumulative engine operating time is now 225 hr. Build number 11 and 12 of engine S/N 1 totaled 28 burning hours and constituted a single assembly of the engine core--the compressor, both turbines, and the gearbox. Build number 11 of engine S/N 1 included a 1:07 hr continuous test at 100% gasifier speed (86,000 rpm). Build number 8 of engine S/N 2 was the first engine test with a ceramic turbine rotor. A mechanical loss test of an engine assembly revealed the actual losses to be near the original design allowance. Component development activity included rig testing of the compressor, combustor, and regenerator. Compressor testing was initiated on a rig modified to control the transfer of heat between flow path, lubricating oil, and structure. Results show successful thermal decoupling of the rig and lubricating/cooling oil. Rig evaluation of a reduced-friction compressor was initiated. Combustor testing covered qualification of ceramic parts for engine use, mapping of operating range limits, and evaluation of a relocated igniter plug. Several seal refinements were tested on the hot regenerator rig. An alternate regenerator disk, extruded MAS, was examined and found to be currently inadequate for the AGT 100 application. Also, a new technique for measuring leakage was explored on the regenerator rig. Ceramic component activity has focused on the development of state-of-the-art material strength characteristics in full-scale hardware. Injection-molded sintered alpha-SiC rotors were produced at Carborundum in an extensive process and tool optimization study.

Ceramic applications in turbine engines

NASA Technical Reports Server (NTRS)

Byrd, J. A.; Janovicz, M. A.; Thrasher, S. R.

1981-01-01

Development testing activities on the 1900 F-configuration ceramic parts were completed, 2070 F-configuration ceramic component rig and engine testing was initiated, and the conceptual design for the 2265 F-configuration engine was identified. Fabrication of the 2070 F-configuration ceramic parts continued, along with burner rig development testing of the 2070 F-configuration metal combustor in preparation for 1132 C (2070 F) qualification test conditions. Shakedown testing of the hot engine simulator (HES) rig was also completed in preparation for testing of a spin rig-qualified ceramic-bladed rotor assembly at 1132 C (2070 F) test conditions. Concurrently, ceramics from new sources and alternate materials continued to be evaluated, and fabrication of 2070 F-configuration ceramic component from these new sources continued. Cold spin testing of the critical 2070 F-configuration blade continued in the spin test rig to qualify a set of ceramic blades at 117% engine speed for the gasifier turbine rotor. Rig testing of the ceramic-bladed gasifier turbine rotor assembly at 108% engine speed was also performed, which resulted in the failure of one blade. The new three-piece hot seal with the nickel oxide/calcium fluoride wearface composition was qualified in the regenerator rig and introduced to engine operation wiwth marginal success.

Experimental Research and Analysis of Rotordynamic Characteristics for a New Kind of Tilting-pad Gas Seal

NASA Astrophysics Data System (ADS)

Cao, Hao; Cao, Xiaoyu; Chen, Fei; Li, Ming; Zhang, Bolin; Wei, Jilong

2017-12-01

This paper presents a new kind of tilting-pad gas seal. This design is introduced to reduce the tangential seal force and to improve the stability of rotor system finally. A seal test rig is set up. The paper compares the leakage between tilting-pad seal and fixed pad seal. The result shows that the leakage ratio of the tilting-pad seal is close to the leakage ratio of the fixed pad seal. The work done by seal force on the cylinder system is calculated as an index of comparison between these two seals. Result shows that the work done by the fixed pad seal is greater than the work done by the tilting-pad seal. Moreover, system damping factor is used to compare the stabilities of these two seals. The impact tests on the cylinder system are done under different conditions. The system damping factors are calculated from the damped waves of system vibration. Test results show that the damping factor of the tilting pad seal is higher than that of the fixed pad seal in both the vertical and the horizontal directions.

A New Tribological Test for Candidate Brush Seal Materials Evaluation

NASA Technical Reports Server (NTRS)

Fellenstein, James A.; Dellacorte, Christopher

1994-01-01

A new tribological test for candidate brush seal materials evaluation has been developed. The sliding contact between the brush seal wires and their mating counterface journal is simulated by testing a small tuft of wire against the outside diameter of a high speed rotating shaft. The test configuration is similar to a standard block on ring geometry. The new tester provides the capability to measure both the friction and wear of candidate wire and counterface materials under controlled loading conditions in the gram to kilogram range. A wide test condition latitude of speeds (1 to 27 m/s), temperatures (25 to 700 C), and loads (0.5 to 10 N) enables the simulation of many of the important tribological parameters found in turbine engine brush seals. This paper describes the new test rig and specimen configuration and presents initial data for candidate seal materials comparing tuft test results and wear surface morphology to field tested seal components.

High Temperature Brush Seal Tuft Testing of Selected Nickel-Chrome and Cobalt-Chrome Superalloys

NASA Technical Reports Server (NTRS)

Fellenstein, James A.; DellaCorte, Christopher; Moore, Kenneth D.; Boyes, Esther

1997-01-01

The tribology of brush seals is of considerable interest to turbine engine designers because bristle wear continues to limit long term seal performance and life. To provide better materials characterization and foster the development of improved seals, NASA Lewis has developed a brush seal tuft tester. In this test, a 'paintbrush' sample tuft is loaded under constant contact pressure against the outside diameter of a rotating journal. With this configuration, load and friction are directly measured and accurate wear measurements are possible. Previously reported research using this facility showed excellent data repeatability and wear morphology similar to published seal data and dynamic rig tests. This paper is an update of the ongoing research into the tribology of brush seals. The effects of wire materials processing on seal wear and the tribological results for three journal coatings are discussed. Included in the materials processing were two nickel-chrome superalloys each processed to two different yield strengths. The results suggest that seal wear is dependent more on material composition than processing conditions.

Advanced Seal Development for Large Industrial Gas Turbines

NASA Technical Reports Server (NTRS)

Chupp, Raymond E.

2006-01-01

Efforts are in progress to develop advanced sealing for large utility industrial gas turbine engines (combustion turbines). Such seals have been under developed for some time for aero gas turbines. It is desired to transition this technology to combustion turbines. Brush seals, film riding face and circumferential seals, and other dynamic and static sealing approaches are being incorporated into gas turbines for aero applications by several engine manufacturers. These seals replace labyrinth or other seals with significantly reduced leakage rates. For utility industrial gas turbines, leakage reduction with advanced sealing can be even greater with the enormous size of the components. Challenges to transitioning technology include: extremely long operating times between overhauls; infrequent but large radial and axial excursions; difficulty in coating larger components; and maintenance, installation, and durability requirements. Advanced sealing is part of the Advanced Turbine Systems (ATS) engine development being done under a cooperative agreement between Westinghouse and the US Department of Energy, Office of Fossil Energy. Seal development focuses on various types of seals in the 501ATS engine both at dynamic and static locations. Each development includes rig testing of candidate designs and subsequent engine validation testing of prototype seals. This presentation gives an update of the ongoing ATS sealing efforts with special emphasis on brush seals.

Experimental studies and performance analyses on polyurethane and nitrile rubber rod seals

NASA Astrophysics Data System (ADS)

Mirza, M.; Temiz, V.; Kamburoǧlu, E.

2012-09-01

The aim of this study is to determine the friction and leakage properties of rod seals made of polyethylene and nitrile rubber with different design geometries, under various pressure and lubricating oil viscosity conditions, in order to make assumptions about their general sealing characteristics and their pros and cons under certain working conditions that involve a range of fluid pressures. The test specimens consist of commercial rod seals of various designs and materials and were mounted on a hard chrome coated shaft subject to reciprocating motion. The test rig is capable of measuring friction force by means of strain measurements on a load cell transmitting the linear motion of a screw shaft to the test shaft. The test results of the reciprocating rod seal samples were evaluated according to leakage amount and friction resistance as a function of materials, design geometries and fluid pressures as well as the lubricating oil viscosity.

High temperature lubricant screening and systems studies

NASA Technical Reports Server (NTRS)

Jones, D. A.

1973-01-01

Four candidate lubricants for next generation aircraft gas turbine application were tested under open atmosphere conditions in a rig simulating an advanced engine 125 mm bore mainshaft thrust bearing position. Testing was conducted at speeds to 24,000 rpm (3,000,000 bearing DN), bearing ring temperature of 500 F, and with 1200 F air and 100 psi differential pressure across the seals installed in a dual tandem arrangement. Test bearing was a 125 mm bore split inner ring, outer race riding angular contact ball bearing under a 3280 lb. thrust load. One lubricant, a type 2 ester, performed extremely well. The mainshaft seal limited the performance. Numerous design improvements for this seal were indicated.

Correction coefficient for see-through labyrinth seal

NASA Astrophysics Data System (ADS)

Hasnedl, Dan; Epikaridis, Premysl; Slama, Vaclav

In a steam turbine design, the flow-part design and blade shapes are influenced by the design mass-flow through each turbine stage. If it would be possible to predict this mass-flow more precisely, it will result in optimized design and therefore an efficiency benefit. This article is concerned with improving the prediction of losses caused by the seal leakage. In the common simulation of the thermodynamic cycle of a steam turbine, analytical formulas are used in order to simulate the seal leakage. Therefore, this article describes an improvement of analytical formulas used in a turbine heat balance calculation. The results are verified by numerical simulations and experimental data from the steam test rig.

Overview of Glenn Mechanical Components Branch Research

NASA Astrophysics Data System (ADS)

Zakrajsek, James

2002-09-01

Mr. James Zakrajsek, chief of the Mechanical Components Branch, gave an overview of research conducted by the branch. Branch members perform basic research on mechanical components and systems, including gears and bearings, turbine seals, structural and thermal barrier seals, and space mechanisms. The research is focused on propulsion systems for present and advanced aerospace vehicles. For rotorcraft and conventional aircraft, we conduct research to develop technology needed to enable the design of low noise, ultra safe geared drive systems. We develop and validate analytical models for gear crack propagation, gear dynamics and noise, gear diagnostics, bearing dynamics, and thermal analyses of gear systems using experimental data from various component test rigs. In seal research we develop and test advanced turbine seal concepts to increase efficiency and durability of turbine engines. We perform experimental and analytical research to develop advanced thermal barrier seals and structural seals for current and next generation space vehicles. Our space mechanisms research involves fundamental investigation of lubricants, materials, components and mechanisms for deep space and planetary environments.

Subterranean drilling and in situ treatment of wastes using a contamination control system and methods relating thereto

DOEpatents

Jessmore, James J.; Loomis, Guy G.; Pettet, Mark C.; Flyckt, Melissa C.

2004-09-28

Systems and methods relating to subterranean drilling while maintaining containment of any contaminants released during the drilling. A thrust block installed over a zone of interest provides an overflow space for retaining any contaminants and excess sealant returns. Negative air pressure may be maintained in the overflow space by a ventilation system. Access ports in the thrust block seal the overflow space from the surrounding environment with a membrane seal. A flexible sack seal in the access port may be connected to a drill shroud prior to drilling, providing containment during drilling after the drill bit penetrates the membrane seal. The drill shroud may be adapted to any industry standard drilling rig and includes a connection conduit for connecting to the flexible sack seal and a flexible enclosure surrounding the drill shaft and of a length to accommodate full extension thereof. Upon withdrawal, the sack seal may be closed off and separated, maintaining containment of the overflow space and the drill shroud.

High-Speed, High-Temperature Finger Seal Test Evaluated

NASA Technical Reports Server (NTRS)

Proctor, Margaret P.

2003-01-01

A finger seal, designed and fabricated by Honeywell Engines, Systems and Services, was tested at the NASA Glenn Research Center at surface speeds up to 1200 ft/s, air temperatures up to 1200 F, and pressures across the seal of 75 psid. These are the first test results obtained with NASA s new High-Temperature, High-Speed Turbine Seal Test Rig (see the photograph). The finger seal is an innovative design recently patented by AlliedSignal Engines, which has demonstrated considerably lower leakage than commonly used labyrinth seals and is considerably cheaper than brush seals. The cost to produce finger seals is estimated to be about half of the cost to produce brush seals. Replacing labyrinth seals with fingers seals at locations that have high-pressure drops in gas turbine engines, typically main engine and thrust seals, can reduce air leakage at each location by 50 percent or more. This directly results in a 0.7- to 1.4-percent reduction in specific fuel consumption and a 0.35- to 0.7-percent reduction in direct operating costs . Because the finger seal is a contacting seal, this testing was conducted to address concerns about its heat generation and life capability at the higher speeds and temperatures required for advanced engines. The test results showed that the seal leakage and wear performance are acceptable for advanced engines.

Full-Scale System for Quantifying Leakage of Docking System Seals for Space Applications

NASA Technical Reports Server (NTRS)

Dunlap, Patrick H., Jr.; Daniels, Christopher C.; Steinetz, Bruce M.; Erker, Arthur H.; Robbie, Malcolm G.; Wasowski, Janice L.; Drlik, Gary J.; Tong, Michael T.; Penney, Nicholas

2007-01-01

NASA is developing a new docking and berthing system to support future space exploration missions to low-Earth orbit, the Moon, and Mars. This mechanism, called the Low Impact Docking System, is designed to connect pressurized space vehicles and structures. NASA Glenn Research Center is playing a key role in developing advanced technology for the main interface seal for this new docking system. The baseline system is designed to have a fully androgynous mating interface, thereby requiring a seal-on-seal configuration when two systems mate. These seals will be approximately 147 cm (58 in.) in diameter. NASA Glenn has designed and fabricated a new test fixture which will be used to evaluate the leakage of candidate full-scale seals under simulated thermal, vacuum, and engagement conditions. This includes testing under seal-on-seal or seal-on-plate configurations, temperatures from -50 to 50 C (-58 to 122 F), operational and pre-flight checkout pressure gradients, and vehicle misalignment (plus or minus 0.381 cm (0.150 in.)) and gapping (up to 0.10 cm (0.040 in.)) conditions. This paper describes the main design features of the test rig and techniques used to overcome some of the design challenges.

Measurements of Pressure Distributions and Force Coefficients in a Squeeze Film Damper. Part 2: Partially Sealed Configuration

NASA Technical Reports Server (NTRS)

Jung, S. Y.; Sanandres, Luis A.; Vance, J. M.

1991-01-01

Experimental results from a partially sealed squeeze film damper (SFD) test rig, executing a circular centered orbit are presented and discussed. A serrated piston ring is installed at the damper exit. This device involves a new sealing concept which produces high damping values while allowing for oil flow to cool the damper. In the partially sealed damper, large cavitation regions are observed in the pressure fields at orbit radii epsilon equals 0.5 and epsilon equals 0.8. The cavitated pressure distributions and the corresponding force coefficients are compared with a cavitated bearing solution. The experimental results show the significance of fluid inertia and vapor cavitation in the operation of squeeze film dampers. Squeeze film Reynolds numbers tested reach up to Re equals 50, spanning the range of contemporary applications.

Static and Dynamic Pressure Distributions in a Short Labyrinth Seal

NASA Technical Reports Server (NTRS)

Millsaps, K. T.; Martinez-Sanchez, M.

1991-01-01

As part of a study into turbine blade tip destabilizing forces, a seals test rig was built in which spin rate, circular whirl rate, direction and amplitude of inlet swirl angle, and eccentricity can all be controlled over wide ranges, and measurements can be made at gap Reynolds numbers up to about 2 x 10(exp 4). This facility is described and preliminary data is presented for a one cavity labyrinth seal with a flat, stator mounted land. The impact of different flow coefficients for the first and second knives on the rotordynamic coefficients was found. While this effect is dominant for the direct forces, it should also be incorporated into calculations of cross forces where it has an impact under many conditions.

Whirl Motion of a Seal Test Rig with Squeeze-Film Dampers

NASA Technical Reports Server (NTRS)

Proctor, Margaret P.; Gunter, Edgar J.

2007-01-01

This paper presents the experimental behavior and dynamic analysis of a high speed test rig with rolling element bearings mounted in squeeze film oil damper bearings. The test rotor is a double overhung configuration with rolling element ball bearings mounted in uncentered squeeze-film oil dampers. The damper design is similar to that employed with various high-speed aircraft HP gas turbines. The dynamic performance of the test rig with the originally installed dampers with an effective damper length of length 0.23-inch was unacceptable. The design speed of 40,000 RPM could not be safely achieved as nonsynchronous whirling at the overhung seal test disk and high amplitude critical speed response at the drive spline section occurred at 32,000 RPM. In addition to the self excited stability and critical speed problems, it was later seen from FFT data analysis, that a region of supersynchronous dead band whirling occurs between 10,000 to 15,000 RPM which can lead to bearing distress and wear. The system was analyzed using both linear and nonlinear techniques. The extended length damper design resulting from the analysis eliminated the rotor subsynchronous whirling, high amplitude critical speed, and the dead band whirling region allowing the system to achieve a speed of 45,000 RPM. However, nonlinear analysis shows that damper lockup could occur with high rotor unbalance at 33,000 RPM, even with the extended squeeze-film dampers. The control of damper lockup will be addressed in a future paper.

The effect of inlet swirl on the dynamics of long annular seals in centrifugal pumps

NASA Technical Reports Server (NTRS)

Ismail, M.; Brown, R. D.; France, D.

1994-01-01

This paper describes additional results from a continuing research program which aims to identify the dynamics of long annular seals in centrifugal pumps. A seal test rig designed at Heriot-Watt University and commissioned at Weir Pumps Research Laboratory in Alloa permits the identification of mass, stiffness, and damping coefficients using a least-squares technique based on the singular value decomposition method. The analysis is carried out in the time domain using a multi-fiequency forcing function. The experimental method relies on the forced excitation of a flexibly supported stator by two hydraulic shakers. Running through the stator embodying two symmetrical balance drum seals is a rigid rotor supported in rolling element bearings. The only physical connection between shaft and stator is the pair of annular gaps filled with pressurized water discharged axially. The experimental coefficients obtained from the tests are compared with theoretical values.

Calculation and measurement of the influence of flow parameters on rotordynamic coefficients in labyrinth seals

NASA Technical Reports Server (NTRS)

Kwanka, K.; Ortinger, W.; Steckel, J.

1994-01-01

First experimental investigations performed on a new test rig are presented. For a staggered labyrinth seal with fourteen cavities the stiffness coefficient and the leakage flow are measured. The experimental results are compared to calculated results which are obtained by a one-volume bulk-flow theory. A perturbation analysis is made for seven terms. It is found out that the friction factors have great impact on the dynamic coefficients. They are obtained by turbulent flow computation by a finite-volume model with the Reynolds equations used as basic equations.

Seal Technology for Liquid Oxygen (LOX) Turbopumps

NASA Technical Reports Server (NTRS)

Shapiro, Wilbur; Hamm, Robert

1985-01-01

Two types of advanced seals for liquid oxygen (LOX) turbopumps were investigated. One was a spiral-groove face seal whose function is to seal high-pressure LOX at the impeller end of the turbopump. The other was a floating-ring, Rayleigh-step, helium buffered seal used to prevent LOX ingress to the turbine side of the unit. For each seal type, two sizes were investigated (50 and 20 mm). A turbine-driven test rig was designed and manufactured, and a test program was completed on the 50 mm floating-ring, Rayleigh-step, helium buffered seal. Significant results were: vaporization in the flow path could cause failure by overheating; therefore, the spiral-groove pumping portion of the seal that provides the fluid film must circulate fluid without disruption if vaporization occurs in the sealing dam. This is successfully accomplished by a pressure-balanced spiral-groove concept that is described. The spiral-groove configuration is affected by turbulence in the fluid film and pressure drops due to fluid inertia at sudden contractions. The net results of these effects are deep grooves, large operating films, and high power loss when compared against seals operating with laminar films. Turbulence and inertia are induced by the high-density and low-viscosity characteristics of LOX. The program clearly pointed out the need to consider system environmental factors such as thermal and centrifugal distortions and rotor vibrations in the seal design.

Development of Advanced Carbon Face Seals for Aircraft Engines

NASA Astrophysics Data System (ADS)

Falaleev, S. V.; Bondarchuk, P. V.; Tisarev, A. Yu

2018-01-01

Modern aircraft gas turbine engines require the development of seals which can operate for a long time with low leakages. The basic type of seals applied for gas turbine engine rotor supports is face seal. To meet the modern requirements of reliability, leak-tightness and weight, low-leakage gas-static and hydrodynamic seals have to be developed. Dry gas seals use both gas-static and hydrodynamic principles. In dry gas seals microgrooves are often used, which ensure the reverse injection of leakages in the sealed cavity. Authors have developed a calculation technique including the concept of coupled hydrodynamic, thermal and structural calculations. This technique allows to calculate the seal performance taking into account the forces of inertia, rupture of the lubricant layer and the real form of the gap. Authors have compared the efficiency of seals with different forms of microgrooves. Results of calculations show that seal with rectangular form of microgrooves has a little gap leading to both the contact of seal surfaces and the wear. Reversible microgrooves have a higher oil mass flow rate, whereas HST micro-grooves have good performance, but they are difficult to produce. Spiral microgrooves have both an acceptable leakages and a high stiffness of liquid layer that is important in terms of ensuring of sealing performance at vibration conditions. Therefore, the spiral grooves were chosen for the developed seal. Based on calculation results, geometric dimensions were chosen to ensure the reliability of the seal operation by creating a guaranteed liquid film, which eliminates the wear of the sealing surfaces. Seals designed were tested both at the test rig and in the engine.

Numerical Analysis of Intra-Cavity and Power-Stream Flow Interaction in Multiple Gas-Turbine Disk-Cavities

NASA Technical Reports Server (NTRS)

Athavale, M. M.; Przekwas, A. J.; Hendricks, R. C.; Steinetz, B. M.

1995-01-01

A numerical analysis methodology and solutions of the interaction between the power stream and multiply-connected multi-cavity sealed secondary flow fields are presented. Flow solutions for a multi-cavity experimental rig were computed and compared with experimental data of Daniels and Johnson. The flow solutions illustrate the complex coupling between the main-path and the cavity flows as well as outline the flow thread that exists throughout the subplatform multiple cavities and seals. The analysis also shows that the de-coupled solutions on single cavities is inadequate. The present results show trends similar to the T-700 engine data that suggests the changes in the CDP seal altered the flow fields throughout the engine and affected the engine performance.

Overview of NASA Glenn Seal Developments

NASA Technical Reports Server (NTRS)

Steinetz, Bruce M.; Proctor, Margaret P.; Dunlap, Patrick H., Jr.; Delgado, Irebert; DeMange, Jeffrey J.; Daniels, Christopher C.; Lattime, Scott B.

2004-01-01

Turbine engine studies have shown that reducing high pressure turbine (HPT) blade tip clearances will reduce fuel burn, lower emissions, retain exhaust gas temperature margin and increase range. Dr. Lattime presented the design and development status of a new Active Clearance Control Test rig aimed at demonstrating advanced ACC approaches and sensors. Mr. Melcher presented controls considerations for turbine active clearance control. Mr. Geisheimer of Radatech presented an overview of their microwave blade tip sensor technology. Microwave tip sensors show promise of operation in the extreme gas temperatures present in the HPT location. Mr. Justak presented an overview of non-contacting seal developments at Advanced Technologies Group. Dr. Braun presented investigations into a non-contacting finger seal under development by NASA GRC and University of Akron. Dr. Stango presented analytical assessments of the effects of flow-induced radial loads on brush seal behavior. Mr. Flaherty presented innovative seal and seal fabrication developments at FlowServ. Mr. Chappel presented abradable seal developments at Technetics. Dr. Daniels presented an overview of NASA GRC s acoustic seal developments. NASA is investigating the ability to harness high amplitude acoustic waves, possible through a new field of acoustics called Resonant Macrosonic Synthesis, to effect a non-contacting, low leakage seal. Dr. Daniels presented early results showing the ability to restrict flow via acoustic pressures. Dr. Athavale presented numerical results simulating the flow blocking capability of a pre-prototype acoustic seal.

Plasma-sprayed zirconia gas path seal technology: A state-of-the-art review

NASA Technical Reports Server (NTRS)

Bill, R. C.

1979-01-01

The benefits derived from application of ceramic materials to high pressure turbine gas path seal components are described and the developmental backgrounds of various approaches are reviewed. The most fully developed approaches are those employing plasma sprayed zirconium oxide as the ceramic material. Prevention of cracking and spalling of the zirconium oxide under cyclic thermal shock conditions imposed by the engine operating cycle is the most immediate problem to be solved before implementation is undertaken. Three promising approaches to improving cyclic thermal shock resistance are described and comparative rig performance of each are reviewed. Advanced concepts showing potential for performance improvements are described.

Light and short arc rubs in rotating machines: Experimental tests and modelling

NASA Astrophysics Data System (ADS)

Pennacchi, P.; Bachschmid, N.; Tanzi, E.

2009-10-01

Rotor-to-stator rub is a non-linear phenomenon which has been analyzed many times in rotordynamics literature, but very often these studies are devoted simply to highlight non-linearities, using very simple rotors, rather than to present reliable models. However, rotor-to-stator rub is actually one of the most common faults during the operation of rotating machinery. The frequency of its occurrence is increasing due to the trend of reducing the radial clearance between the seal and the rotor in modern turbine units, pumps and compressors in order to increase efficiency. Often the rub occurs between rotor and seals and the analysis of the phenomenon cannot set aside the consideration of the different relative stiffness. This paper presents some experimental results obtained by means of a test rig in which rub conditions of real machines are reproduced. In particular short arc rubs are considered and the shaft is stiffer than the obstacle. Then a model, suitable to be employed for real rotating machinery, is presented and the simulations obtained are compared with the experimental results. The model is able to reproduce the behaviour of the test rig.

Large Eddy Simulations of the Tilted Rig Experiment: A Two-dimensional Rayleigh-Taylor Instability Case

NASA Astrophysics Data System (ADS)

Rollin, Bertrand; Denissen, Nicholas A.; Reisner, Jon M.; Andrews, Malcolm J.

2012-11-01

The tilted rig experiment is a derivative of the rocket rig experiment designed to investigate turbulent mixing induced by the Rayleigh-Taylor (RT) instability. A tank containing two fluids of different densities is accelerated downwards between two parallel guiding rods by rocket motors. The acceleration is such that the pressure and density gradients face opposite directions at the fluids interface, creating a Rayleigh-Taylor unstable configuration. The rig is tilted such that the tank is initially at an angle and the acceleration is not perpendicular to the fluids interface when the rockets fire. This results in a two dimensional Rayleigh-Taylor instability case where the fluids experience RT mixing and a bulk overturning motion. The tilted rig is therefore a valuable experiment to help calibrating two-dimensional mixing models. Large Eddy Simulations of the tilted rig experiments will be compared to available experimental results. A study of the behavior of turbulence variables relevant to turbulence modeling will be presented. LA-UR 12-23829. This work was performed for the U.S. Department of Energy by Los Alamos National Laboratory under Contract No.DEAC52- 06NA2-5396.

Rotary Engine Friction Test Rig Development Report

DTIC Science & Technology

2011-12-01

fundamental research is needed to understand the friction characteristics of the rotary engine that lead to accelerated wear and tear on the seals...that includes a turbocharger . Once the original GT-Suite model is validated, the turbocharger model will be more accurate. This validation will...prepare for turbocharger and fuel-injector testing, which will lead to further development and calibration of the model. Further details are beyond the

Hercules 265 rapid response: Immediate ecosystem impacts of a natural gas blowout incident

NASA Astrophysics Data System (ADS)

Weber, Sarah C.; Peterson, Leigha; Battles, Jessica J.; Roberts, Brian J.; Peterson, Richard N.; Hollander, David J.; Chanton, Jeffrey P.; Joye, Samantha B.; Montoya, Joseph P.

2016-07-01

In late July 2013, the Hercules 265 drilling rig in the Northern Gulf of Mexico experienced a catastrophic loss of control. Large quantities of natural gas spewed into the environment for 2 days before the well self-sealed through down-hole collapse below the seafloor. Ecosystem Impacts of Oil and Gas Inputs to the Gulf (ECOGIG) and collaborating Gulf of Mexico Research Initiative (GoMRI) consortia mounted a rapid response cruise to characterize the waters around the Hercules 265 rig, beginning just 4 days after the blowout. Our analysis showed an immediate microbial response to the elevated concentrations of methane in the water column, as evidenced by the drawdown of oxygen to hypoxic conditions, the incorporation of methane-derived carbon into particles, and measurable rates of methane-assimilation and nitrogen-fixation. Additionally, radium isotope measurements allowed us to constrain the timescale of bottom water exposure to the influence of the rig. A second sampling by the Center for Integrated Modeling and Analysis of Gulf Ecosystems (C-IMAGE) consortium indicated that the ecosystem had returned to near pre-blowout conditions within one month.

Modeling the rubbing contact in honeycomb seals

NASA Astrophysics Data System (ADS)

Fischer, Tim; Welzenbach, Sarah; Meier, Felix; Werner, Ewald; kyzy, Sonun Ulan; Munz, Oliver

2018-03-01

Metallic honeycomb labyrinth seals are commonly used as sealing systems in gas turbine engines. Because of their capability to withstand high thermo-mechanical loads and oxidation, polycrystalline nickel-based superalloys, such as Hastelloy X and Haynes 214, are used as sealing material. In addition, these materials must exhibit a tolerance against rubbing between the rotating part and the stationary seal component. The tolerance of the sealing material against rubbing preserves the integrity of the rotating part. In this article, the rubbing behavior at the rotor-stator interface is considered numerically. A simulation model is incorporated into the commercial finite element code ABAQUS/explicit and is utilized to simulate a simplified rubbing process. A user-defined interaction routine between the contact surfaces accounts for the thermal and mechanical interfacial behavior. Furthermore, an elasto-plastic constitutive material law captures the extreme temperature conditions and the damage behavior of the alloys. To validate the model, representative quantities of the rubbing process are determined and compared with experimental data from the literature. The simulation results correctly reproduce the observations made on a test rig with a reference stainless steel material (AISI 304). A parametric study using the nickel-based superalloys reveals a clear dependency of the rubbing behavior on the sliding and incursion velocity. Compared to each other, the two superalloys studied exhibit a different rubbing behavior.

Sea Bed Drilling Technology MARUM-MeBo: Overview on recent scientific drilling campaigns and technical developments

NASA Astrophysics Data System (ADS)

Freudenthal, Tim; Bergenthal, Markus; Bohrmann, Gerhard; Pape, Thomas; Kopf, Achim; Huhn-Frehers, Katrin; Gohl, Karsten; Wefer, Gerold

2017-04-01

The MARUM-MeBo (abbreviation for Meeresboden-Bohrgerät, the German expression for seafloor drill rig) is a robotic drilling system that is developed since 2004 at the MARUM Center for Marine Environmental Sciences at the University of Bremen in close cooperation with Bauer Maschinen GmbH and other industry partners. The MARUM-MeBo drill rigs can be deployed from multipurpose research vessel like, RV MARIA S. MERIAN, RV METEOR, RV SONNE and RV POLARSTERN and are used for getting long cores both in soft sediments as well as hard rocks in the deep sea. The first generation drill rig, the MARUM-MeBo70 is dedicated for a drilling depth of more than 70 m (Freudenthal and Wefer, 2013). Between 2005 and 2016 it was deployed on 17 research expeditions and drilled about 3 km into different types of geology including carbonate and crystalline rocks, gas hydrates, glacial tills, sands and gravel, glacial till and hemipelagic mud with an average recovery rate of about 70 %. We used the development and operational experiences of MARUM-MeBo70 for the development of a second generation drill rig MARUM-MeBo200. This drill rig is dedicated for conducting core drilling down to 200 m below sea floor. After successful sea trials in the North Sea in October 2014 the MeBo200 was used on a scientific expedition on the research vessel RV SONNE (SO247) in March/April 2016. During 12 deployments we drilled altogether 514 m in hemipelagic sediments with volcanic ashes as well as in muddy and sandy slide deposits off New Zealand. The average core recovery was about 54%. The maximum drilling depth was 105 m below sea floor. Developments for the MeBo drilling technology include the development of a pressure core barrel that was successfully deployed on two research expeditions so far. Bore hole logging adds to the coring capacity. Several autonomous logging probes have been developed in the last years for a deployment with MeBo in the logging while tripping mode - a sonic probe measuring in situ p-wave velocity being the latest development. Various bore hole monitoring systems where developed and deployed with the MeBo system. They allow for long-term monitoring of pressure variability within the sealed bore holes. References: Freudenthal, T and Wefer, G (2013) Drilling cores on the sea floor with the remote-controlled sea floor drilling rig MeBo. Geoscientific Instrumentation, Methods and Data Systems, 2(2). 329-337. doi:10.5194/gi-2-329-2013

Further Characterization of an Active Clearance Control Concept

NASA Technical Reports Server (NTRS)

Taylor, Shawn C.; Steinetz, Bruce M.; Oswald, Jay J.

2007-01-01

A new test chamber and precision hydraulic actuation system were incorporated into an active clearance control (ACC) test rig at NASA Glenn Research Center. Using the improved system, a fast-acting, mechanically-actuated, ACC concept was evaluated at engine simulated temperatures and pressure differentials up to 1140 F and 120 psig, on the basis of secondary seal leakage and kinematic controllability. During testing, the ACC concept tracked a simulated flight clearance transient profile at 1140 F, 120 psig, with a maximum error of only 0.0012 in. Comparison of average dynamic leakage of the system with average static leakage did not show significant differences between the two operating conditions. Calculated effective clearance values for the rig were approximately 0.0002 in. at 120 psig, well below the industry specified effective clearance threshold of 0.001 in.

A calibration rig for multi-component internal strain gauge balance using the new design-of-experiment (DOE) approach

NASA Astrophysics Data System (ADS)

Nouri, N. M.; Mostafapour, K.; Kamran, M.

2018-02-01

In a closed water-tunnel circuit, the multi-component strain gauge force and moment sensor (also known as balance) are generally used to measure hydrodynamic forces and moments acting on scaled models. These balances are periodically calibrated by static loading. Their performance and accuracy depend significantly on the rig and the method of calibration. In this research, a new calibration rig was designed and constructed to calibrate multi-component internal strain gauge balances. The calibration rig has six degrees of freedom and six different component-loading structures that can be applied separately and synchronously. The system was designed based on the applicability of formal experimental design techniques, using gravity for balance loading and balance positioning and alignment relative to gravity. To evaluate the calibration rig, a six-component internal balance developed by Iran University of Science and Technology was calibrated using response surface methodology. According to the results, calibration rig met all design criteria. This rig provides the means by which various methods of formal experimental design techniques can be implemented. The simplicity of the rig saves time and money in the design of experiments and in balance calibration while simultaneously increasing the accuracy of these activities.

Seals Research at Texas A/M University

NASA Technical Reports Server (NTRS)

Morrison, Gerald L.

1991-01-01

The Turbomachinery Laboratory at Texas A&M has been providing experimental data and computational codes for the design seals for many years. The program began with the development of a Halon based seal test rig. This facility provided information about the effective stiffness and damping in whirling seals. The Halon effectively simulated cryogenic fluids. Another test facility was developed (using air as the working fluid) where the stiffness and damping matrices can be determined. This data was used to develop bulk flow models of the seal's effect upon rotating machinery; in conjunction with this research, a bulk flow model for calculation of performance and rotordynamic coefficients of annular pressure seals of arbitrary non-uniform clearance for barotropic fluids such as LH2, LOX, LN2, and CH4 was developed. This program is very efficient (fast) and converges for very large eccentricities. Currently, work is being performed on a bulk flow analysis of the effects of the impeller-shroud interaction upon the stability of pumps. The data was used along with data from other researchers to develop an empirical leakage prediction code for MSFC. Presently, the flow field inside labyrinth and annular seals are being studied in detail. An advanced 3-D Doppler anemometer system is being used to measure the mean velocity and entire Reynolds stress tensor distribution throughout the seals. Concentric and statically eccentric seals were studied; presently, whirling seals are being studied. The data obtained are providing valuable information about the flow phenomena occurring inside the seals, as well as a data base for comparison with numerical predictions and for turbulence model development. A finite difference computer code was developed for solving the Reynolds averaged Navier Stokes equation inside labyrinth seals. A multi-scale k-epsilon turbulence model is currently being evaluated. A new seal geometry was designed and patented using a computer code. A large scale, 2-D seal flow visualization facility is also being developed.

Feasibility of SiC composite structures for 1644 deg gas turbine seal applications

NASA Technical Reports Server (NTRS)

Darolia, R.

1979-01-01

The feasibility of silicon carbide composite structures was evaluated for 1644 K gas turbine seal applications. The silicon carbide composites evaluated consisted of Si/SiC Silcomp (Trademark) - and sintered silicon carbide as substrates, both with attached surface layers containing BN as an additive. A total of twenty-eight candidates with variations in substrate type and density, and layer chemistry, density, microstructure, and thickness were evaluated for abradability, cold particle erosion resistance, static oxidation resistance, ballistic impact resistance, and fabricability. The BN-free layers with variations in density and pore size were later added for evaluation. The most promising candidates were evaluated for Mach 1.0 gas oxidation/erosion resistance from 1477 K to 1644 K. The as-fabricated rub layers did not perform satisfactorily in the gas oxidation/erosion tests. However, preoxidation was found to be beneficial in improving the hot gas erosion resistance. Overall, the laboratory and rig test evaluations show that material properties are suitable for 1477 K gas turbine seal applications.

Compliant seal development

NASA Technical Reports Server (NTRS)

Hendricks, Robert C.

1993-01-01

The compliant metallic seal combines the noncontact feature of the labyrinth seal, the low leakage of a mechanical seal, and the compliant nature of the brush seal. It consists of several thin metallic elements or leaves mounted within a ring which is press fit into the housing, and in form, sort of resembles a lip seal sections wiping the shaft. A second set of overlapping cover leaves are placed on top of the shaft riding leaves which reduces leakage and provides stiffness. The leaves can be straight or angle cut. The shaft riding fingers are designed with mismatched curvature to provide lift off similar to the Rayleigh lift pads in mechanical seals with leading edge clearances nearly twice those of the trailing edge as as shown by Fleming to be optimal for gas flows in convergent seal passages. Leading edge clearances range from 300 to 500 microinches. Balance pockets beneath the leaves provide fluid film feed to the 'Rayleigh lift' surface and the proper balance ratio (mechanical seal) when combined with the static pressure and film pressure. The leaves flex in the radial direction and accommodate thermomechanical behavior as well as axial motion and angular misalignment. In the static mode, there is a net closing force on the leaves. The seals were tested to 70 psi at speeds to 16,000 rpm or surface speeds to 330 fps and temperatures from ambient to 440 F. A slow cycle through the rig critical at 10,000 rpm induced a radial vibration response of 0.004 to 0.005 inch were accommodated by the seal. Preliminary performance data are encouraging demonstrating hydrodynamic liftoff and noncontacting operation at pressure and speeds typical of gas turbine engines. The leakage performance data are significantly better than commercial labyrinth and brush seals which should be expected as this design incorporates the features of the low leakage face or mechanical seal along with the flexibility of the brush configuration.

Compliant seal development

NASA Astrophysics Data System (ADS)

Hendricks, Robert C.

1993-10-01

The compliant metallic seal combines the noncontact feature of the labyrinth seal, the low leakage of a mechanical seal, and the compliant nature of the brush seal. It consists of several thin metallic elements or leaves mounted within a ring which is press fit into the housing, and in form, sort of resembles a lip seal sections wiping the shaft. A second set of overlapping cover leaves are placed on top of the shaft riding leaves which reduces leakage and provides stiffness. The leaves can be straight or angle cut. The shaft riding fingers are designed with mismatched curvature to provide lift off similar to the Rayleigh lift pads in mechanical seals with leading edge clearances nearly twice those of the trailing edge as as shown by Fleming to be optimal for gas flows in convergent seal passages. Leading edge clearances range from 300 to 500 microinches. Balance pockets beneath the leaves provide fluid film feed to the 'Rayleigh lift' surface and the proper balance ratio (mechanical seal) when combined with the static pressure and film pressure. The leaves flex in the radial direction and accommodate thermomechanical behavior as well as axial motion and angular misalignment. In the static mode, there is a net closing force on the leaves. The seals were tested to 70 psi at speeds to 16,000 rpm or surface speeds to 330 fps and temperatures from ambient to 440 F. A slow cycle through the rig critical at 10,000 rpm induced a radial vibration response of 0.004 to 0.005 inch were accommodated by the seal. Preliminary performance data are encouraging demonstrating hydrodynamic liftoff and noncontacting operation at pressure and speeds typical of gas turbine engines. The leakage performance data are significantly better than commercial labyrinth and brush seals which should be expected as this design incorporates the features of the low leakage face or mechanical seal along with the flexibility of the brush configuration.

Circumferential pressure distributions in a model labyrinth seal

NASA Technical Reports Server (NTRS)

Leong, Y. M. M. S.; Brown, R. D.

1982-01-01

A research program to isolate and study leakage flow through labyrinth glands was initiated. Circumferential pressure distributions were measured in the labyrinth glands with geometry appropriate to the high pressure labyrinths in large steam turbines. Knowledge of this pressure distribution is essential as it is this unequal pressure field that results in the destabilizing force. Parameters that are likely to affect the pressure distributions are incorporated into the test rig. Some preliminary pressure profiles are presented.

Engine-Scale Combustor Rig Designed, Fabricated, and Tested for Combustion Instability Control Research

NASA Technical Reports Server (NTRS)

DeLaat, John C.; Breisacher, Kevin J.

2000-01-01

Low-emission combustor designs are prone to combustor instabilities. Because active control of these instabilities may allow future combustors to meet both stringent emissions and performance requirements, an experimental combustor rig was developed for investigating methods of actively suppressing combustion instabilities. The experimental rig has features similar to a real engine combustor and exhibits instabilities representative of those in aircraft gas turbine engines. Experimental testing in the spring of 1999 demonstrated that the rig can be tuned to closely represent an instability observed in engine tests. Future plans are to develop and demonstrate combustion instability control using this experimental combustor rig. The NASA Glenn Research Center at Lewis Field is leading the Combustion Instability Control program to investigate methods for actively suppressing combustion instabilities. Under this program, a single-nozzle, liquid-fueled research combustor rig was designed, fabricated, and tested. The rig has many of the complexities of a real engine combustor, including an actual fuel nozzle and swirler, dilution cooling, and an effusion-cooled liner. Prior to designing the experimental rig, a survey of aircraft engine combustion instability experience identified an instability observed in a prototype engine as a suitable candidate for replication. The frequency of the instability was 525 Hz, with an amplitude of approximately 1.5-psi peak-to-peak at a burner pressure of 200 psia. The single-nozzle experimental combustor rig was designed to preserve subcomponent lengths, cross sectional area distribution, flow distribution, pressure-drop distribution, temperature distribution, and other factors previously found to be determinants of burner acoustic frequencies, mode shapes, gain, and damping. Analytical models were used to predict the acoustic resonances of both the engine combustor and proposed experiment. The analysis confirmed that the test rig configuration and engine configuration had similar longitudinal acoustic characteristics, increasing the likelihood that the engine instability would be replicated in the rig. Parametric analytical studies were performed to understand the influence of geometry and condition variations and to establish a combustion test plan. Cold-flow experiments verified that the design values of area and flow distributions were obtained. Combustion test results established the existence of a longitudinal combustion instability in the 500-Hz range with a measured amplitude approximating that observed in the engine. Modifications to the rig configuration during testing also showed the potential for injector independence. The research combustor rig was developed in partnership with Pratt & Whitney of West Palm Beach, Florida, and United Technologies Research Center of East Hartford, Connecticut. Experimental testing of the combustor rig took place at United Technologies Research Center.

Fatigue Crack Growth Behavior Evaluation of Grainex Mar-M 247 for NASA's High Temperature, High Speed Turbine Seal Test Rig

NASA Technical Reports Server (NTRS)

Delgado, Irebert R.; Steinetz, Bruce M.; Rimnac, Clare M.; Lewandowski, John J.

2008-01-01

The fatigue crack growth behavior of Grainex Mar-M 247 is evaluated for NASA s Turbine Seal Test Facility. The facility is used to test air-to-air seals primarily for use in advanced jet engine applications. Because of extreme seal test conditions of temperature, pressure, and surface speeds, surface cracks may develop over time in the disk bolt holes. An inspection interval is developed to preclude catastrophic disk failure by using experimental fatigue crack growth data. By combining current fatigue crack growth results with previous fatigue strain-life experimental work, an inspection interval is determined for the test disk. The fatigue crack growth life of the NASA disk bolt holes is found to be 367 cycles at a crack depth of 0.501 mm using a factor of 2 on life at maximum operating conditions. Combining this result with previous fatigue strain-life experimental work gives a total fatigue life of 1032 cycles at a crack depth of 0.501 mm. Eddy-current inspections are suggested starting at 665 cycles since eddy current detection thresholds are currently at 0.381 mm. Inspection intervals are recommended every 50 cycles when operated at maximum operating conditions.

Long Life Ball-Bearings for Use in Satellites in Sealed Containers

DTIC Science & Technology

1976-03-01

Nxtending or compresing the shaft by this aount produces a 10 036 change of load of approximately 2000M (450 lb), the housing is of course much stiffer...race, with its preload plunger, and the housing cylinder. The rig temperature is sensed by a thermocouille which is embedded into the motor housing...IC Fig7 LUbriation regims Inn rams REPORT DOCUMENTATION PAGE overall security classification of this page UNCLASSIFIED A s far j - possib!k th is page

A Hierarchical Mechanism of RIG-I Ubiquitination Provides Sensitivity, Robustness and Synergy in Antiviral Immune Responses.

PubMed

Sun, Xiaoqiang; Xian, Huifang; Tian, Shuo; Sun, Tingzhe; Qin, Yunfei; Zhang, Shoutao; Cui, Jun

2016-07-08

RIG-I is an essential receptor in the initiation of the type I interferon (IFN) signaling pathway upon viral infection. Although K63-linked ubiquitination plays an important role in RIG-I activation, the optimal modulation of conjugated and unanchored ubiquitination of RIG-I as well as its functional implications remains unclear. In this study, we determined that, in contrast to the RIG-I CARD domain, full-length RIG-I must undergo K63-linked ubiquitination at multiple sites to reach full activity. A systems biology approach was designed based on experiments using full-length RIG-I. Model selection for 7 candidate mechanisms of RIG-I ubiquitination inferred a hierarchical architecture of the RIG-I ubiquitination mode, which was then experimentally validated. Compared with other mechanisms, the selected hierarchical mechanism exhibited superior sensitivity and robustness in RIG-I-induced type I IFN activation. Furthermore, our model analysis and experimental data revealed that TRIM4 and TRIM25 exhibited dose-dependent synergism. These results demonstrated that the hierarchical mechanism of multi-site/type ubiquitination of RIG-I provides an efficient, robust and optimal synergistic regulatory module in antiviral immune responses.

A Hierarchical Mechanism of RIG-I Ubiquitination Provides Sensitivity, Robustness and Synergy in Antiviral Immune Responses

PubMed Central

Sun, Xiaoqiang; Xian, Huifang; Tian, Shuo; Sun, Tingzhe; Qin, Yunfei; Zhang, Shoutao; Cui, Jun

2016-01-01

RIG-I is an essential receptor in the initiation of the type I interferon (IFN) signaling pathway upon viral infection. Although K63-linked ubiquitination plays an important role in RIG-I activation, the optimal modulation of conjugated and unanchored ubiquitination of RIG-I as well as its functional implications remains unclear. In this study, we determined that, in contrast to the RIG-I CARD domain, full-length RIG-I must undergo K63-linked ubiquitination at multiple sites to reach full activity. A systems biology approach was designed based on experiments using full-length RIG-I. Model selection for 7 candidate mechanisms of RIG-I ubiquitination inferred a hierarchical architecture of the RIG-I ubiquitination mode, which was then experimentally validated. Compared with other mechanisms, the selected hierarchical mechanism exhibited superior sensitivity and robustness in RIG-I-induced type I IFN activation. Furthermore, our model analysis and experimental data revealed that TRIM4 and TRIM25 exhibited dose-dependent synergism. These results demonstrated that the hierarchical mechanism of multi-site/type ubiquitination of RIG-I provides an efficient, robust and optimal synergistic regulatory module in antiviral immune responses. PMID:27387525

A Hierarchical Mechanism of RIG-I Ubiquitination Provides Sensitivity, Robustness and Synergy in Antiviral Immune Responses

NASA Astrophysics Data System (ADS)

Sun, Xiaoqiang; Xian, Huifang; Tian, Shuo; Sun, Tingzhe; Qin, Yunfei; Zhang, Shoutao; Cui, Jun

2016-07-01

RIG-I is an essential receptor in the initiation of the type I interferon (IFN) signaling pathway upon viral infection. Although K63-linked ubiquitination plays an important role in RIG-I activation, the optimal modulation of conjugated and unanchored ubiquitination of RIG-I as well as its functional implications remains unclear. In this study, we determined that, in contrast to the RIG-I CARD domain, full-length RIG-I must undergo K63-linked ubiquitination at multiple sites to reach full activity. A systems biology approach was designed based on experiments using full-length RIG-I. Model selection for 7 candidate mechanisms of RIG-I ubiquitination inferred a hierarchical architecture of the RIG-I ubiquitination mode, which was then experimentally validated. Compared with other mechanisms, the selected hierarchical mechanism exhibited superior sensitivity and robustness in RIG-I-induced type I IFN activation. Furthermore, our model analysis and experimental data revealed that TRIM4 and TRIM25 exhibited dose-dependent synergism. These results demonstrated that the hierarchical mechanism of multi-site/type ubiquitination of RIG-I provides an efficient, robust and optimal synergistic regulatory module in antiviral immune responses.

Validation of the Small Hot Jet Acoustic Rig for Jet Noise Research

NASA Technical Reports Server (NTRS)

Bridges, James; Brown, Clifford A.

2005-01-01

The development and acoustic validation of the Small Hot Jet Aeroacoustic Rig (SHJAR) is documented. Originally conceived to support fundamental research in jet noise, the rig has been designed and developed using the best practices of the industry. While validating the rig for acoustic work, a method of characterizing all extraneous rig noise was developed. With this in hand, the researcher can know when the jet data being measured is being contaminated and design the experiment around this limitation. Also considered is the question of uncertainty, where it is shown that there is a fundamental uncertainty of 0.5dB or so to the best experiments, confirmed by repeatability studies. One area not generally accounted for in the uncertainty analysis is the variation which can result from differences in initial condition of the nozzle shear layer. This initial condition was modified and the differences in both flow and sound were documented. The bottom line is that extreme caution must be applied when working on small jet rigs, but that highly accurate results can be made independent of scale.

Radioimmunoguided surgery for colorectal cancer.

PubMed

Bertsch, D J; Burak, W E; Young, D C; Arnold, M W; Martin, E W

1996-05-01

Operations for patients with colorectal cancer are based on traditions established by historical experience. Radioimmunoguided surgery (RIGS) provides new information that challenges these traditions. Thirty-two patients with primary colorectal cancer underwent RIGS after being injected with anti-TAG-72 murine monoclonal antibody CC49 labeled with iodine-125. Sixteen of the patients had all gross tumor and RIGS-positive tissue removed (RIGS-negative group), and 16 had only traditional extirpation of the tumor because RIGS-positive tissue was too diffuse (RIGS-positive group). In the 16 patients having all RIGS-positive tissue removed, five had traditional regional en bloc resections and 11 had additional extraregional tissues resected. Identification of extraregional disease added two liver resections and 25 lymphadenectomies: 10 of the gastrohepatic ligament, five celia axis, six retroperitoneal, and four iliac. With a median follow-up of 37 months, survival in the RIGS-negative group is 100%. In 14 of 16 patients (87.5%) there is no evidence of disease. In the RIGS-positive group, follow-up shows 14 of 16 patients are dead and two are alive with disease (p < 0.0001). These results suggest that RIGS identifies patterns of disease dissemination different from those identified by traditional staging techniques. Removal of additional RIGS-positive tissues in nontraditional areas may improve survival.

Influence of rubbing on rotor dynamics, part 1

NASA Technical Reports Server (NTRS)

Muszynska, Agnes; Bently, Donald E.; Franklin, Wesley D.; Hayashida, Robert D.; Kingsley, Lori M.; Curry, Arthur E.

1989-01-01

The results of analytical and experimental research on rotor-to-stationary element rubbing in rotating machines are presented. A characterization of physical phenomena associated with rubbing, as well as a literature survey on the subject of rub is given. The experimental results were obtained from two rubbing rotor rigs: one, which dynamically simulates the space shuttle main engine high pressure fuel turbopump (HPFTP), and the second one, much simpler, a two-mode rotor rig, designed for more generic studies on rotor-to-stator rubbing. Two areas were studied: generic rotor-to-stator rub-related dynamic phenomena affecting rotating machine behavior and applications to the space shuttle HPFTP. An outline of application of dynamic stiffness methodology for identification of rotor/bearing system modal parameters is given. The mathematical model of rotor/bearing/seal system under rub condition is given. The computer program was developed to calculate rotor responses. Compared with experimental results the computed results prove an adequacy of the model.

Methodology for the in vitro evaluation of the delivery efficiency from valved holding chambers with facemasks.

PubMed

Xu, Zhen; Hsu, Wenchi; von Hollen, Dirk; Viswanath, Ashwin; Nikander, Kurt; Dalby, Richard

2014-08-01

In vitro performance studies of valved holding chamber (VHC)-facemask systems are a cost-effective means of circumventing potentially confounding clinical variables. This article reports results of an in vitro investigation into VHC-facemask performance, using three age-specific soft anatomical model (SAM) faces, under clinically relevant conditions. A potentially standardized method was developed to assess VHC-facemask seal leakage, and evaluate the in vitro delivery efficiency of conventional and antistatic VHC-facemask systems. A custom-built test rig and VHC cradles were used to position the VHC-facemask systems against the SAM faces, with a constant, reproducible force. A standardized simulated pediatric breathing pattern (tidal volume = 155 mL; inhalation:exhalation ratio = 40:60; 25 breaths/min) was utilized. Percent facemask seal leakage, percent delivered dose, and the effect of different numbers of simulated breaths (2 to 8) were investigated. Of the VHC-facemask systems tested, the OptiChamber Diamond VHC with LiteTouch facemask (Diamond) system had the lowest percent seal leakage with each SAM face. Percent seal leakage from the other VHC-facemask systems was similar with SAM0 and SAM2 faces; the AeroChamber Plus Z-Stat VHC with ComfortSeal facemask (AC Z-Stat) system had a substantially greater percent seal leakage with the SAM1 face. Regardless of the number of simulated breaths, the Diamond system delivered the greatest mean percent delivered dose, with the lowest coefficient of variation, with each SAM face. Percent delivered dose did not correlate well with seal leakage, particularly for VHC-facemask systems with high seal leakage. The electrostatic properties of the VHCs appeared to influence drug delivery. This study describes a potentially standardized method for the evaluation of VHC-facemask systems. Use of this method enabled a comprehensive investigation into the influence of clinically relevant variables, including age-specific facial anatomy, number of simulated breaths, and seal leakage, on the delivery efficiency of several commercially available VHC-facemask systems.

Experimental Analysis of Mast Lifting and Bending Forces on Vibration Patterns Before and After Pinion Reinstallation in an OH-58 Transmission Test Rig

NASA Technical Reports Server (NTRS)

Huff, Edward M.; Barszcz, Eric; Turner, Irem Y.; Lewicki, David; Decker, Harry; Norvig, Peter (Technical Monitor)

1999-01-01

As part of a cooperative research program between NASA Ames Research Center, NASA Glenn Research Center, and the U.S. Army Laboratories, a series of experiments are being performed on the 500 HP OH-58a Transmission Test Rig at NASA Glenn Research Center. The findings reported in this paper were drawn from Phase 1 of a two-phase experiment, and are focused on the vibration response of an undamaged pinion gear and planetary system operating in situ in the transmission test rig. Phase 2 of the experiment, which is reported elsewhere, introduced a seeded fault into the pinion gear and tracked its progress in real-time. Based on methods presented here, further experimental research will be conducted to examine planetary system faults.

Monitoring Well Installation and Groundwater Sampling and Analysis Plan at the USARC Training Reserve, 84th Division, Milwaukee, Wisconsin

DTIC Science & Technology

1988-11-01

paint chips at the sampling site. 0 Clean water tanks, pumps, mud pans, hoses, including hoses and tanks used to transfer water from source to drill rig...TO’ LCA , Filll I F’APCr,;I~- € C/ " rKL2PIrlA , ATTFNrIGN TO SMOKING. ALCOHOLF MFDrICATIONP AND FXPOSI.RE TO CARCINOGENS.1 ENERAL MEDICAl. HISTORY...A. General: 1. Place samples in core trough for visual inspection. After logging, place selected samples in sample jars or wood core boxes. 2. Seal

Mini-CORK observatories using the MeBo seafloor drill rig - a new development for long-term data acquisition and sampling in shallow boreholes

NASA Astrophysics Data System (ADS)

Kopf, A.; Freudenthal, T.; Ratmeyer, V.; Bergenthal, M.; Renken, J.; Zabel, M.; Wefer, G.

2011-12-01

State of the art technology for long-term monitoring of fluid migration within the sea floor is the sealing of a borehole with a Circulation Obviation Retrofit Kit (CORK) after sensor installation and/or fluid sampling devices within the drill string. However, the combined used of a drilling vessels and a remotely operated drilling (ROV) required for a CORK installation in the deep sea is a costly exercise that limits the number of monitoring stations installed. Robotic sea floor drill rigs are a cost effective alternative for shallow drillings down to 50-100 m below sea floor. Here we present a Mini-CORK system that is developed for installation with the sea floor drill rig MeBo. This rig was developed at MARUM Research Centre, University of Bremen in 2005 and can sample the sea floor in water depths up to 2000 m. The MeBo is deployed on the seabed and remotely controlled from the vessel. All required drill tools for wire-line core drilling down to 70 m below sea floor are stored on two rotating magazines and can be loaded below the top drive drill head for assembling the drill string. For one of the upcoming cruises with RV Sonne offshore Japan (Nankai Trough accretionary prism), MeBo will be used for the first time to place observatories. Two different designs have been developed. The first, relatively simple long-term device resembles a MeBo drill rod in its geometry, and contains a pressure and temperature transducer in the borehole plus an identical pair of transducers for seafloor reference. The device also contains a data logger, battery unit, and an acoustic modem so that data can be downloaded at any time from a ship of opportunity. The key element at the base of the observatory rod is a seal at the conical thread to separate the borehole hydraulically from the overlying water body. It is realized by an adapter, which also contains a hotstab hydraulic connection and an electrical connection. The second observatory device is a seafloor unit, which replaces part of the first unit and which is deployed by ROV. In essence, the upper portion of the former observatory is taken away by ROV, and an umbilical containing hydraulic lines and tubing to withdraw formation water from the borehole is plugged into the hotstab female adapter by ROV. At the far end, the umbilical is connected to a seafloor unit with battery power, data logger, P and T transducers, and the same acoustic modem as the former one. In addition, the latter contains osmo samplers and biological chambers (FLOCS) for in situ sampling and experiments. After the envisaged deployment period, the entire unit is replaced while an identical one is prepared on deck and lowered from the vessel. In theory, the MeBo hole infinitely serves as an access to depth since no electronic, but only tubing is lowered into the (open) hole. In summary, long-term borehole installations with MeBo offer an affordable way to measure key physical properties over time and sample the formation fluids for geochemistry and microbiology (in case of the second, ROV-deployed CORK).

A Three-Stage Counter Current Leaching Rig for the Senior Laboratory.

ERIC Educational Resources Information Center

Davies, Wayne A.

1989-01-01

Described is a reliable and predictable laboratory experiment which represents the result of an integrated approach to design with an emphasis on teaching. Notes the flat-celled rig has logged over 100 hours service in 2 years without any problems. (MVL)

Chemical Looping Autothermal Reforming at a 120 kW Pilot Rig

NASA Astrophysics Data System (ADS)

Bofhàr-Nordenkampf, Johannes; Pröll, Tobias; Kolbitsch, Philipp; Hofbauer, Hermann

Chemical looping with selective oxygen transport allows two step combustion or autothermal reforming without mixing of fuel and air. The reactor system consists of two reactors, an air reactor and a fuel reactor with a suitable oxygen carrier that transports the necessary oxygen for operation. In the present study, a highly active nickel based oxygen carrier is tested in a novel dual circulating fluidized bed (DCFB) system at a scale of 120 kW fuel power. The mean particle size of the oxygen carrier is 120 μm and the pilot rig is fueled with natural gas. For the investigated oxygen carrier high CH4 conversion is achieved. Air/fuel ratio is varied at three different fuel reactor temperatures. For chemical looping reforming one can observe synthesis gas composition close to thermodynamic equilibrium. In spite of the fact that no additional steam has been added to the fuel besides the one present through steam fluidization of the loop seals, coke formation does not occur at global stoichiometric air/fuel ratios above 0.46.

A New Analysis Tool Assessment for Rotordynamic Modeling of Gas Foil Bearings

NASA Technical Reports Server (NTRS)

Howard, Samuel A.; SanAndres, Luis

2010-01-01

Gas foil bearings offer several advantages over traditional bearing types that make them attractive for use in high-speed turbomachinery. They can operate at very high temperatures, require no lubrication supply (oil pumps, seals, etc.), exhibit very long life with no maintenance, and once operating airborne, have very low power loss. The use of gas foil bearings in high-speed turbomachinery has been accelerating in recent years, although the pace has been slow. One of the contributing factors to the slow growth has been a lack of analysis tools, benchmarked to measurements, to predict gas foil bearing behavior in rotating machinery. To address this shortcoming, NASA Glenn Research Center (GRC) has supported the development of analytical tools to predict gas foil bearing performance. One of the codes has the capability to predict rotordynamic coefficients, power loss, film thickness, structural deformation, and more. The current paper presents an assessment of the predictive capability of the code, named XLGFBTH (Texas A&M University). A test rig at GRC is used as a simulated case study to compare rotordynamic analysis using output from the code to actual rotor response as measured in the test rig. The test rig rotor is supported on two gas foil journal bearings manufactured at GRC, with all pertinent geometry disclosed. The resulting comparison shows that the rotordynamic coefficients calculated using XLGFBTH represent the dynamics of the system reasonably well, especially as they pertain to predicting critical speeds.

Numerical Methodology for Coupled Time-Accurate Simulations of Primary and Secondary Flowpaths in Gas Turbines

NASA Technical Reports Server (NTRS)

Przekwas, A. J.; Athavale, M. M.; Hendricks, R. C.; Steinetz, B. M.

2006-01-01

Detailed information of the flow-fields in the secondary flowpaths and their interaction with the primary flows in gas turbine engines is necessary for successful designs with optimized secondary flow streams. Present work is focused on the development of a simulation methodology for coupled time-accurate solutions of the two flowpaths. The secondary flowstream is treated using SCISEAL, an unstructured adaptive Cartesian grid code developed for secondary flows and seals, while the mainpath flow is solved using TURBO, a density based code with capability of resolving rotor-stator interaction in multi-stage machines. An interface is being tested that links the two codes at the rim seal to allow data exchange between the two codes for parallel, coupled execution. A description of the coupling methodology and the current status of the interface development is presented. Representative steady-state solutions of the secondary flow in the UTRC HP Rig disc cavity are also presented.

Meals on Wheels? A Decade of Megafaunal Visual and Acoustic Observations from Offshore Oil & Gas Rigs and Platforms in the North and Irish Seas.

PubMed

Todd, Victoria Louise Georgia; Warley, Jane Clare; Todd, Ian Boyer

2016-01-01

A decade of visual and acoustic detections of marine megafauna around offshore Oil & Gas (O&G) installations in the North and Irish Seas are presented. Marine megafauna activity was monitored visually and acoustically by Joint Nature Conservation Committee (JNCC) qualified and experienced Marine Mammal Observers (MMO) and Passive Acoustic Monitoring (PAM) Operators respectively, with real-time towed PAM in combination with industry standard software, PAMGuard. Monitoring was performed during routine O&G industrial operations for underwater noise mitigation purposes, and to ensure adherence to regulatory guidelines. Incidental sightings by off-effort MMOs and installation crew were also reported. Visual and acoustic monitoring spanned 55 non-consecutive days between 2004 and 2014. A total of 47 marine mammal sightings were recorded by MMOs on dedicated watch, and 10 incidental sightings of marine megafauna were reported over 10 years. Species included: harbour porpoise (Phocoena phocoena), Atlantic white-sided dolphin (Lagenorhynchus acutus), white beaked dolphin (Lagenorhynchus albirostris), common dolphin (Delphinus delphis), minke whale (Balaenoptera acutorostrata), common seal (Phoca vitulina), grey seal (Halichoerus grypus) and, basking shark (Cetorhinus maximus). Passive Acoustic Monitoring was conducted on two occasions in 2014; 160 PAM hours over 12 days recorded a total of 308 individual clicks identified as harbour porpoises. These appear to be the first such acoustic detections obtained from a North Sea drilling rig whilst using a typically configured hydrophone array designed for towing in combination with real-time PAMGuard software. This study provides evidence that marine megafauna are present around mobile and stationary offshore O&G installations during routine operational activities. On this basis, Environmental Impact Assessments (EIAs) for decommissioning O&G platforms should be carried-out on a case-by-case basis, and must include provisions for hitherto overlooked marine megafauna.

Experimental Analysis of Mast Lifting and Bending Forces on Vibration Patterns Before and After Pinion Reinstallation in an OH-58 Transmission Test Rig

NASA Technical Reports Server (NTRS)

Huff, Edward M.; Lewicki, David G.; Tumer, Irem Y.; Decker, Harry; Barszez, Eric; Zakrajsek, James J.; Norvig, Peter (Technical Monitor)

2000-01-01

As part of a collaborative research program between NASA Ames Research Center (ARC), NASA Glenn Research Center (GRC), and the US Army Laboratory, a series of experiments is being performed in GRC's 500 HP OH-58 Transmission Test Rig facility and ARC's AH-I Cobra and OH-58c helicopters. The findings reported in this paper were drawn from Phase-I of a two-phase test-rig experiment, and are focused on the vibration response of an undamaged pinion gear operating in the transmission test rig. To simulate actual flight conditions, the transmission system was run at three torque levels, as well as two mast lifting and two mast bending levels. The test rig was also subjected to disassembly and reassembly of the main pinion housing to simulate the effect of maintenance operations. An analysis of variance based on the total power of the spectral distribution indicates the relative effect of each experimental factor, including Wong interactions with torque. Reinstallation of the main pinion assembly is shown to introduce changes in the vibration signature, suggesting the possibility of a strong effect of maintenance on HUMS design and use. Based on these results, further research will be conducted to compare these vibration responses with actual OH58c helicopter transmission vibration patterns.

Towards the control of the modal energy transfer in transverse mode instabilities

NASA Astrophysics Data System (ADS)

Stihler, Christoph; Jauregui, Cesar; Tünnermann, Andreas; Limpert, Jens

2018-02-01

Thermally-induced refractive index gratings (RIG) in high-power fiber laser systems lead to transverse mode instabilities (TMI) above a certain average power threshold. The effect of TMI is currently the main limitation for the further average power scaling of fiber lasers and amplifiers with nearly diffraction-limited beam quality. In this work we experimentally investigate, for the first time, the growth of the RIG strength by introducing a phase-shift between the RIG and the modal interference pattern in a fiber amplifier. The experiments reveal that the RIG is strong enough to couple energy between different transverse modes even at powers significantly below the TMI threshold, provided that the introduced phase-shift is high enough. This indicates that, as the strength of the RIG further increases with increasing average output power, the RIG becomes more and more sensitive to even small noise-induced phase-shifts, which ultimately trigger TMI. Furthermore, it is shown that a beam cleaning also occurs when a positive phase-shift is introduced, even above the TMI threshold. This finding will pave the way for the development of a new class of mitigation strategies for TMI, which key feature is the control of the introduced phase-shift.

Experimental verification of vapor deposition rate theory in high velocity burner rigs

NASA Technical Reports Server (NTRS)

Gokoglu, Suleyman A.; Santoro, Gilbert J.

1985-01-01

The main objective has been the experimental verification of the corrosive vapor deposition theory in high-temperature, high-velocity environments. Towards this end a Mach 0.3 burner-rig appartus was built to measure deposition rates from salt-seeded (mostly Na salts) combustion gases on the internally cooled cylindrical collector. Deposition experiments are underway.

Investigation of flow in axial turbine stage without shroud-seal

NASA Astrophysics Data System (ADS)

Straka, Petr; Němec, Martin; Jelínek, Thomáš

2015-05-01

This article deals with investigation of the influence of the radial gaps on the efficiency of the axial turbine stage. The investigation was carried out for the axial stage of the low-power turbine with the drum-type rotor without the shroud. In this configuration the flow through the radial gap under the hub-end of the stator blades and above the tip-end of the rotor blades leads to generation of the strong secondary flows, which decrease the efficiency of the stage. This problem was studied by experiment as well as by numerical modelling. The experiment was performed on the test rig equipped with the water brake dynamometer, torque meter and rotatable stator together with the linear probe manipulator. Numerical modelling was carried out for both the steady flow using the "mixing plane" interface and the unsteady flow using the "sliding mesh" interface between the stator and rotor wheels. The influence of the radial gap was studied in two configuration a) positive and b) negative overlapping of the tip-ends of the rotor blades. The efficiency of the axial stage in dependence on the expansion ratio, velocity ratio and the configuration as well as the details of the flow fields are presented in this paper.

Function of duck RIG-I in induction of antiviral response against IBDV and avian influenza virus on chicken cells.

PubMed

Shao, Qiang; Xu, Wenpin; Yan, Li; Liu, Jinhua; Rui, Lei; Xiao, Xiao; Yu, Xiaoxue; Lu, Yanan; Li, Zandong

2014-10-13

The avian influenza (AI) H9N2 virus and IBDV are two major problems in the poultry industry. They have been prevalent among domestic poultry in Asia for many years and have caused considerable economic losses. Retinoic-acid-induced gene I (RIG-I) is a cytoplasmic sensor of dsRNA and ssRNA. It can detect Encephalomyocarditis virus (EMCV) and vesicular stomatitis virus (VSV) in human cells, influenza virus in duck leads to production of IFN-β and IFN-stimulated antiviral genes and reductions in the replication of RNA virus. Chickens, which lack RIG-I, are more sensitive to influenza virus than ducks. However, little is known about the roles of duck RIG-I (dRIG-I) in the detection of IBDV and AI H9N2 in chicken cells DF-1. The purpose of this study was to examine the function of dRIG-I in the recognition of IBDV Ts strain and H9N2 A/Chicken/Shandong/ZB/2007(ZB07) and in the induction of antiviral gene expression to gain an understanding of antiviral ability of dRIG-I in chicken cells against dsRNA virus IBDV and ssRNA virus ZB07. After challenge with the IBDV Ts strain and ZB07 the expression levels of Type I IFN (IFN-β and IFN-α) and IFN-induced antiviral genes (Mx and PKR) were significantly up-regulated in dRIG-I-transfected DF-1cells compared with the empty-vector-transfected control. dRIG-I knockdown experiments further proved that dRIG-I is essential to sensing IBDV and ZB07 in duck embryo fibroblasts (DEF). Growth curves showed that dRIG-I repressed the replication of IBDV and almost blunted the growth of ZB07 in DF-1. Apoptosis analysis revealed that dRIG-I increase the number of the survival cells after IBDV Ts strain or ZB07 infection relative to the empty-vector-transfected control. These results indicate that dRIG-I can up-regulates type I IFN and reduce viral gene expression and viral replication and protect chicken cells from virus-induced apoptosis during ZB07 and IBDV infection. Copyright © 2014 Elsevier B.V. All rights reserved.

rigor mortis encodes a novel nuclear receptor interacting protein required for ecdysone signaling during Drosophila larval development.

PubMed

Gates, Julie; Lam, Geanette; Ortiz, José A; Losson, Régine; Thummel, Carl S

2004-01-01

Pulses of the steroid hormone ecdysone trigger the major developmental transitions in Drosophila, including molting and puparium formation. The ecdysone signal is transduced by the EcR/USP nuclear receptor heterodimer that binds to specific response elements in the genome and directly regulates target gene transcription. We describe a novel nuclear receptor interacting protein encoded by rigor mortis (rig) that is required for ecdysone responses during larval development. rig mutants display defects in molting, delayed larval development, larval lethality, duplicated mouth parts, and defects in puparium formation--phenotypes that resemble those seen in EcR, usp, E75A and betaFTZ-F1 mutants. Although the expression of these nuclear receptor genes is essentially normal in rig mutant larvae, the ecdysone-triggered switch in E74 isoform expression is defective. rig encodes a protein with multiple WD-40 repeats and an LXXLL motif, sequences that act as specific protein-protein interaction domains. Consistent with the presence of these elements and the lethal phenotypes of rig mutants, Rig protein interacts with several Drosophila nuclear receptors in GST pull-down experiments, including EcR, USP, DHR3, SVP and betaFTZ-F1. The ligand binding domain of betaFTZ-F1 is sufficient for this interaction, which can occur in an AF-2-independent manner. Antibody stains reveal that Rig protein is present in the brain and imaginal discs of second and third instar larvae, where it is restricted to the cytoplasm. In larval salivary gland and midgut cells, however, Rig shuttles between the cytoplasm and nucleus in a spatially and temporally regulated manner, at times that correlate with the major lethal phase of rig mutants and major switches in ecdysone-regulated gene expression. Taken together, these data indicate that rig exerts essential functions during larval development through gene-specific effects on ecdysone-regulated transcription, most likely as a cofactor for one or more nuclear receptors. Furthermore, the dynamic intracellular redistribution of Rig protein suggests that it may act to refine spatial and temporal responses to ecdysone during development.

Meals on Wheels? A Decade of Megafaunal Visual and Acoustic Observations from Offshore Oil & Gas Rigs and Platforms in the North and Irish Seas

PubMed Central

Todd, Victoria Louise Georgia; Todd, Ian Boyer

2016-01-01

A decade of visual and acoustic detections of marine megafauna around offshore Oil & Gas (O&G) installations in the North and Irish Seas are presented. Marine megafauna activity was monitored visually and acoustically by Joint Nature Conservation Committee (JNCC) qualified and experienced Marine Mammal Observers (MMO) and Passive Acoustic Monitoring (PAM) Operators respectively, with real-time towed PAM in combination with industry standard software, PAMGuard. Monitoring was performed during routine O&G industrial operations for underwater noise mitigation purposes, and to ensure adherence to regulatory guidelines. Incidental sightings by off-effort MMOs and installation crew were also reported. Visual and acoustic monitoring spanned 55 non-consecutive days between 2004 and 2014. A total of 47 marine mammal sightings were recorded by MMOs on dedicated watch, and 10 incidental sightings of marine megafauna were reported over 10 years. Species included: harbour porpoise (Phocoena phocoena), Atlantic white-sided dolphin (Lagenorhynchus acutus), white beaked dolphin (Lagenorhynchus albirostris), common dolphin (Delphinus delphis), minke whale (Balaenoptera acutorostrata), common seal (Phoca vitulina), grey seal (Halichoerus grypus) and, basking shark (Cetorhinus maximus). Passive Acoustic Monitoring was conducted on two occasions in 2014; 160 PAM hours over 12 days recorded a total of 308 individual clicks identified as harbour porpoises. These appear to be the first such acoustic detections obtained from a North Sea drilling rig whilst using a typically configured hydrophone array designed for towing in combination with real-time PAMGuard software. This study provides evidence that marine megafauna are present around mobile and stationary offshore O&G installations during routine operational activities. On this basis, Environmental Impact Assessments (EIAs) for decommissioning O&G platforms should be carried-out on a case-by-case basis, and must include provisions for hitherto overlooked marine megafauna. PMID:27078153

Terrain Characterization for Trafficability

DTIC Science & Technology

1993-06-01

pensive and less time-consuming. Although carefully raphy, on vehicle operation. This report focuses on the controlled laboratory tests may be more...relating indentation to soil strength. on a portable test rig or on an off-road vehicle where it A series of controlled experiments to determine the is... Controls and setting values for hydraulic pressures and flow Figure 10. Wheel are test rig (after Wasterhund 1990). 7 Vertical Proximity L ock-Oit~u

Small Hot Jet Acoustic Rig Validation

NASA Technical Reports Server (NTRS)

Brown, Cliff; Bridges, James

2006-01-01

The Small Hot Jet Acoustic Rig (SHJAR), located in the Aeroacoustic Propulsion Laboratory (AAPL) at the NASA Glenn Research Center in Cleveland, Ohio, was commissioned in 2001 to test jet noise reduction concepts at low technology readiness levels (TRL 1-3) and develop advanced measurement techniques. The first series of tests on the SHJAR were designed to prove its capabilities and establish the quality of the jet noise data produced. Towards this goal, a methodology was employed dividing all noise sources into three categories: background noise, jet noise, and rig noise. Background noise was directly measured. Jet noise and rig noise were separated by using the distance and velocity scaling properties of jet noise. Effectively, any noise source that did not follow these rules of jet noise was labeled as rig noise. This method led to the identification of a high frequency noise source related to the Reynolds number. Experiments using boundary layer treatment and hot wire probes documented this noise source and its removal, allowing clean testing of low Reynolds number jets. Other tests performed characterized the amplitude and frequency of the valve noise, confirmed the location of the acoustic far field, and documented the background noise levels under several conditions. Finally, a full set of baseline data was acquired. This paper contains the methodology and test results used to verify the quality of the SHJAR rig.

Development of a turbojet engine gearbox test rig for prognostics and health management

NASA Astrophysics Data System (ADS)

Rezaei, Aida; Dadouche, Azzedine

2012-11-01

Aircraft engine gearboxes represent one of the many critical systems/elements that require special attention for longer and safer operation. Reactive maintenance strategies are unsuitable as they usually imply higher repair costs when compared to condition based maintenance. This paper discusses the main prognostics and health management (PHM) approaches, describes a newly designed gearbox experimental facility and analyses preliminary data for gear prognosis. The test rig is designed to provide full capabilities of performing controlled experiments suitable for developing a reliable diagnostic and prognostic system. The rig is based on the accessory gearbox of the GE J85 turbojet engine, which has been slightly modified and reconfigured to replicate real operating conditions such as speeds and loads. Defect to failure tests (DTFT) have been run to evaluate the performance of the rig as well as to assess prognostic metrics extracted from sensors installed on the gearbox casing (vibration and acoustic). The paper also details the main components of the rig and describes the various challenges encountered. Successful DTFT results were obtained during an idle engine performance test and prognostic metrics associated with the sensor suite were evaluated and discussed.

Post-Test Analysis of 11% Break at PSB-VVER Experimental Facility using Cathare 2 Code

NASA Astrophysics Data System (ADS)

Sabotinov, Luben; Chevrier, Patrick

The best estimate French thermal-hydraulic computer code CATHARE 2 Version 2.5_1 was used for post-test analysis of the experiment “11% upper plenum break”, conducted at the large-scale test facility PSB-VVER in Russia. The PSB rig is 1:300 scaled model of VVER-1000 NPP. A computer model has been developed for CATHARE 2 V2.5_1, taking into account all important components of the PSB facility: reactor model (lower plenum, core, bypass, upper plenum, downcomer), 4 separated loops, pressurizer, horizontal multitube steam generators, break section. The secondary side is represented by recirculation model. A large number of sensitivity calculations has been performed regarding break modeling, reactor pressure vessel modeling, counter current flow modeling, hydraulic losses, heat losses. The comparison between calculated and experimental results shows good prediction of the basic thermal-hydraulic phenomena and parameters such as pressures, temperatures, void fractions, loop seal clearance, etc. The experimental and calculation results are very sensitive regarding the fuel cladding temperature, which show a periodical nature. With the applied CATHARE 1D modeling, the global thermal-hydraulic parameters and the core heat up have been reasonably predicted.

Localization of a continuous CO2 leak from an isotropic flat-surface structure using acoustic emission detection and near-field beamforming techniques

NASA Astrophysics Data System (ADS)

Yan, Yong; Cui, Xiwang; Guo, Miao; Han, Xiaojuan

2016-11-01

Seal capacity is of great importance for the safety operation of pressurized vessels. It is crucial to locate the leak hole timely and accurately for reasons of safety and maintenance. This paper presents the principle and application of a linear acoustic emission sensor array and a near-field beamforming technique to identify the location of a continuous CO2 leak from an isotropic flat-surface structure on a pressurized vessel in the carbon capture and storage system. Acoustic signals generated by the leak hole are collected using a linear high-frequency sensor array. Time-frequency analysis and a narrow-band filtering technique are deployed to extract effective information about the leak. The impacts of various factors on the performance of the localization technique are simulated, compared and discussed, including the number of sensors, distance between the leak hole and sensor array and spacing between adjacent sensors. Experiments were carried out on a laboratory-scale test rig to assess the effectiveness and operability of the proposed method. The results obtained suggest that the proposed method is capable of providing accurate and reliable localization of a continuous CO2 leak.

Sea Spray and Icing in the Emerging Open Water of the Arctic Ocean

DTIC Science & Technology

2013-06-17

can create copious amounts of sea spray. We anticipate that structures placed in shallow water— wind turbines or drilling rigs, for instance— will...anticipate that structures placed in shallow water— wind turbines or drilling rigs, for instance—will, therefore, experience more episodes of freezing...concentrations of wind -generated sea spray and the resulting spray icing on offshore structures, such as wind turbines and exploration, drilling, and production

High-temperature erosion of plasma-sprayed, yttria-stabilized zirconia in a simulated turbine environment

NASA Technical Reports Server (NTRS)

Hanschuh, R. F.

1984-01-01

A series of rig calibration and high temperature tests simulating gas path seal erosion in turbine engines were performed at three impingement angles and at three downstream locations. Plasma sprayed, yttria stablized zirconia specimens were tested. Steady state erosion curves presented for 19 test specimens indicate a brittle type of material erosion despite scanning electron microscopy evidence of plastic deformation. Steady state erosion results were not sensitive to downstream location but were sensitive to impingement angle. At difference downstream locations specimen surface temperature varied from 1250 to 1600 C (2280 to 2900 F) and particle velocity varied from 260 to 320 m/s (850 to 1050 ft/s). The mass ratio of combustion products to erosive grit material was typically 240.

Laboratory-based experiments to investigate the impact of glyphosate-containing herbicide on pollution attenuation and biodegradation in a model pervious paving system.

PubMed

Mbanaso, F U; Coupe, S J; Charlesworth, S M; Nnadi, E O

2013-01-01

An experimental investigation was carried out to determine the effect of glyphosate-containing herbicides (GCHs) on the hydrocarbon retention and biodegradation processes known to occur in pervious pavement systems (PPSs). The PPS test rigs were based on the four-layered design detailed in CIRIA C582. This enabled the pollutant retention capacity of the PPS and biodegradation of retained pollutants by microorganisms to be investigated. The use of test rigs also enabled the impact of GCH on PPS eukaryotic organisms to be studied, by the monitoring of protist bioindicators. Results showed that GCH disrupted hydrocarbon retention by the geotextiles relative to rigs with mineral oil only added, as 9.3% and 24.5% of added hydrocarbon were found in herbicide only rigs and herbicide plus oil rigs respectively. In previous studies, PPS contaminated by mineral oil had been shown to retain 98.7% of added oils and over several weeks, biodegrade this oil in situ. Where GCH was added to experimental models, much higher concentrations of heavy metals, including Pb, Cu, and Zn, were released from the PPS in effluent, particularly where GCH and mineral oil were added together. The source of the majority of the metal contamination was thought to be the used engine oil. The herbicide generally increased the total activity of microbial communities in rig systems and had a stimulating effect on bacterial and fungal population numbers. Although the protists, which are part of the microbial community directly or indirectly responsible for biodegradation, were initially strongly affected by the herbicide, they showed resilience by quickly recovering and increasing their population compared with rigs without added herbicide, including the rigs with mineral oil added to them. However, the presence of herbicide was associated with a decrease in the species richness of recorded protist taxa and a predominance of robust, cosmopolitan or ubiquitous protist genera. Copyright © 2012 Elsevier Ltd. All rights reserved.

History of Space Shuttle Main Engine Turbopump Bearing Testing at the Marshall Space Flight Center

NASA Technical Reports Server (NTRS)

Gibson, Howard; Thom, Robert; Moore, Chip; Haluck, Dave

2010-01-01

The Space Shuttle is propelled into orbit by two solid rocket motors and three liquid fed main engines. After the solid motors fall away, the shuttle engines continue to run for a total time of 8 minutes. These engines are fed propellants by low and high pressure turbopumps. A critical part of the turbopump is the main shaft that supports the drive turbine and the pump inducer and impeller. Rolling element bearings hold the shaft in place during rotation. If the bearings were to fail, the shaft would move, allowing components to rub in a liquid oxygen or hydrogen environment, which could have catastrophic results. These bearings are required to spin at very high speeds, support radial and axial loads, and have high wear resistance without the benefit of a conventional means of lubrication. The Rocketdyne built Shuttle turbopumps demonstrated their capability to perform during launches; however, the seven hour life requirement was not being met. One of the limiting factors was the bearings. In the late 1970's, an engineering team was formed at the Marshall Space Flight Center (MSFC), to develop a test rig and plan for testing the Shuttle s main engine high pressure oxygen turbopump (HPOTP) bearings. The goals of the program were to better understand the operation of bearings in a cryogenic environment and to further develop and refine existing computer models used to predict the operational limits of these bearings. In 1982, testing began in a rig named the Bearing and Seal Material Tester or BSMT as it was commonly called. The first testing investigated the thermal margin and thermal runaway limits of the HPOTP bearings. The test rig was later used to explore potential bearing improvements in the area of increased race curvatures, new cage materials for better lubrication, new wear resistant rolling element materials, and other ideas to improve wear life. The most notable improvements during this tester s time was the incorporation of silicon nitride balls and bronze filled polytetrafluoroethylene (PTFE) cage inserts into the bearings and the anchoring of the SHABERTH bearing model and SINDA thermal computer model for cryogenic bearing analysis. In the mid 1990's, Pratt and Whitney (P&W) won the contract to deliver new high pressure turbopumps for the Shuttle s engines. P&W used two new bearing materials for the rings, Cronidur 30 and AISI 9310 steel and testing was needed on these new materials. A test rig had been designed and delivered to MSFC for testing hydrostatic bearings but with the need by Pratt to validate their bearings, the rig was reconfigured for testing of two ball bearings or a ball bearing and a roller bearing. The P&W bearings are larger than the Rocketdyne bearings and could not be installed in the BSMT. This new test rig was called the LH2 test rig and began operation in 1995. The LH2 test rig accumulated 75,000 seconds of run time in hydrogen. This test rig was valuable in two areas: validating the use of silicon nitride balls and rollers in Alternate Turbopump Development (ATD) bearings, which Pratt eventually used, and in proving the robustness of the balls and rollers after river marks appeared on the surface of the rolling elements. Individual test reports have been presented at conferences and symposiums throughout the years. This paper is a comprehensive report of all the bearing testing done at Marshall. It represents thousands of hours of dedication and labor in all engineering and technical fields that made this program a success.

Backbone resonance assignments of the PRYSPRY domain of TRIM25.

PubMed

Kong, Chen; Penumutchu, Srinivasa R; Hung, Kuo-Wei; Huang, Huiying; Lin, Tianwei; Yu, Chin

2015-10-01

TRIM25 is a member of the tripartite motif (TRIM) family and has been implicated in the regulation of innate immune signaling via the RIG-I (retinoic acid-inducible gene-I) pathway for antiviral defense. As the essential first step towards the structural and functional characterization of the TRIM25/RIG-I interaction, the backbone resonance of the PRYSPRY domain of TRIM25 is assigned here based on triple-resonance experiments using uniformly [(2)H, (13)C, (15)N]-labeled protein.

Experimental verification of corrosive vapor deposition rate theory in high velocity burner rigs

NASA Technical Reports Server (NTRS)

Gokoglu, S. A.; Santoro, G. J.

1986-01-01

The ability to predict deposition rates is required to facilitate modelling of high temperature corrosion by fused salt condensates in turbine engines. A corrosive salt vapor deposition theory based on multicomponent chemically frozen boundary layers (CFBL) has been successfully verified by high velocity burner rig experiments. The experiments involved internally air-impingement cooled, both rotating full and stationary segmented cylindrical collectors located in the crossflow of sodium-seeded combustion gases. Excellent agreement is found between the CFBL theory an the experimental measurements for both the absolute amounts of Na2SO4 deposition rates and the behavior of deposition rate with respect to collector temperature, mass flowrate (velocity) and Na concentration.

Experimental verification of corrosive vapor deposition rate theory in high velocity burner rigs

NASA Technical Reports Server (NTRS)

Gokoglu, Suleyman A.; Santoro, Gilbert J.

1986-01-01

The ability to predict deposition rates is required to facilitate modelling of high temperature corrosion by fused salt condensates in turbine engines. A corrosive salt vapor deposition theory based on multicomponent chemically frozen boundary layers (CFBL) has been successfully verified by high velocity burner rig experiments. The experiments involved internally air-impingement cooled, both rotating full and stationary segmented cylindrical collectors located in the crossflow of sodium-seeded combustion gases. Excellent agreement is found between the CFBL theory and the experimental measurements for both the absolute amounts of Na2SO4 deposition rates and the behavior of deposition rate with respect to collector temperature, mass flowrate (velocity) and Na concentration.

Ubiquitin-mediated modulation of the cytoplasmic viral RNA sensor RIG-I.

PubMed

Oshiumi, Hiroyuki; Matsumoto, Misako; Seya, Tsukasa

2012-01-01

RIG-I-like receptors, including RIG-I, MDA5 and LGP2, recognize cytoplasmic viral RNA. The RIG-I protein consists of N-terminal CARDs, central RNA helicase and C-terminal domains. RIG-I activation is regulated by ubiquitination. Three ubiquitin ligases target the RIG-I protein. TRIM25 and Riplet ubiquitin ligases are positive regulators of RIG-I and deliver the K63-linked polyubiquitin moiety to RIG-I CARDs and the C-terminal domain. RNF125, another ubiquitin ligase, is a negative regulator of RIG-I and mediates K48-linked polyubiquitination of RIG-I, leading to the degradation of the RIG-I protein by proteasomes. The K63-linked polyubiquitin chains of RIG-I are removed by a deubiquitin enzyme, CYLD. Thus, CYLD is a negative regulator of RIG-I. Furthermore, TRIM25 itself is regulated by ubiquitination. HOIP and HOIL proteins are ubiquitin ligases and are also known as linear ubiquitin assembly complexes (LUBACs). The TRIM25 protein is ubiquitinated by LUBAC and then degraded by proteasomes. The splice variant of RIG-I encodes a protein that lacks the first CARD of RIG-I, and the variant RIG-I protein is not ubiquitinated by TRIM25. Therefore, ubiquitin is the key regulator of the cytoplasmic viral RNA sensor RIG-I.

JT9D-70/59 Improved High Pressure Turbine Active Clearance Control System. [for specific fuel consumption improvement

NASA Technical Reports Server (NTRS)

Gaffin, W. O.

1979-01-01

The JT9D-70/59 high pressure turbine active clearance control system was modified to provide reduction of blade tip clearance when the system is activated during cruise operation. The modification increased the flow capacity and air impingement effectiveness of the cooling air manifold to augment turbine case shrinkage capability, and increased responsiveness of the airseal clearance to case shrinkage. The simulated altitude engine testing indicated a significant improvement in specific fuel consumption with the modified system. A 1000 cycle engine endurance test showed no unusual wear or performance deterioration effects on the engine or the clearance control system. Rig tests indicated that the air impingement and seal support configurations used in the engine tests are near optimum.

Theory versus experiment for the rotordynamic coefficients of labyrinth gas seals. II - A comparison to experiment

NASA Technical Reports Server (NTRS)

Childs, D. W.; Scharrer, J. K.

1987-01-01

An experimental test facility is used to measure the leakage and rotordynamic coefficients of teeth-on-rotor and teeth-on-stator labyrinth gas seals. The test results are presented along with the theoretically predicted values for the two seal configurations at three different radial clearances and shaft speeds to 16,000 cpm. The test results show that the theory accurately predicts the cross-coupled stiffness for both seal configurations and shows improvement in the prediction of the direct damping for the teeth-on-rotor seal. The theory fails to predict a decrease in the direct damping coefficient for an increase in the radial clearance for the teeth-on-stator seal.

Modal simulation of gearbox vibration with experimental correlation

NASA Technical Reports Server (NTRS)

Choy, Fred K.; Ruan, Yeefeng F.; Zakrajsek, James J.; Oswald, Fred B.

1992-01-01

A newly developed global dynamic model was used to simulate the dynamics of a gear noise rig at NASA Lewis Research Center. Experimental results from the test rig were used to verify the analytical model. In this global dynamic model, the number of degrees of freedom of the system are reduced by transforming the system equations of motion into modal coordinates. The vibration of the individual gear-shaft system are coupled through the gear mesh forces. A three-dimensional, axial-lateral coupled, bearing model was used to couple the casing structural vibration to the gear-rotor dynamics. The coupled system of modal equations is solved to predict the resulting vibration at several locations on the test rig. Experimental vibration data was compared to the predictions of the global dynamic model. There is excellent agreement between the vibration results from analysis and experiment.

Hepatitis C Virus Reveals a Novel Early Control in Acute Immune Response

PubMed Central

Arnaud, Noëlla; Dabo, Stéphanie; Akazawa, Daisuke; Fukasawa, Masayoshi; Shinkai-Ouchi, Fumiko; Hugon, Jacques; Wakita, Takaji; Meurs, Eliane F.

2011-01-01

Recognition of viral RNA structures by the intracytosolic RNA helicase RIG-I triggers induction of innate immunity. Efficient induction requires RIG-I ubiquitination by the E3 ligase TRIM25, its interaction with the mitochondria-bound MAVS protein, recruitment of TRAF3, IRF3- and NF-κB-kinases and transcription of Interferon (IFN). In addition, IRF3 alone induces some of the Interferon-Stimulated Genes (ISGs), referred to as early ISGs. Infection of hepatocytes with Hepatitis C virus (HCV) results in poor production of IFN despite recognition of the viral RNA by RIG-I but can lead to induction of early ISGs. HCV was shown to inhibit IFN production by cleaving MAVS through its NS3/4A protease and by controlling cellular translation through activation of PKR, an eIF2α-kinase containing dsRNA-binding domains (DRBD). Here, we have identified a third mode of control of IFN induction by HCV. Using HCVcc and the Huh7.25.CD81 cells, we found that HCV controls RIG-I ubiquitination through the di-ubiquitine-like protein ISG15, one of the early ISGs. A transcriptome analysis performed on Huh7.25.CD81 cells silenced or not for PKR and infected with JFH1 revealed that HCV infection leads to induction of 49 PKR-dependent genes, including ISG15 and several early ISGs. Silencing experiments revealed that this novel PKR-dependent pathway involves MAVS, TRAF3 and IRF3 but not RIG-I, and that it does not induce IFN. Use of PKR inhibitors showed that this pathway requires the DRBD but not the kinase activity of PKR. We then demonstrated that PKR interacts with HCV RNA and MAVS prior to RIG-I. In conclusion, HCV recruits PKR early in infection as a sensor to trigger induction of several IRF3-dependent genes. Among those, ISG15 acts to negatively control the RIG-I/MAVS pathway, at the level of RIG-I ubiquitination.These data give novel insights in the machinery involved in the early events of innate immune response. PMID:22022264

Conventional protein kinase C-α (PKC-α) and PKC-β negatively regulate RIG-I antiviral signal transduction.

PubMed

Maharaj, Natalya P; Wies, Effi; Stoll, Andrej; Gack, Michaela U

2012-02-01

Retinoic acid-inducible gene I (RIG-I) is a key sensor for viral RNA in the cytosol, and it initiates a signaling cascade that leads to the establishment of an interferon (IFN)-mediated antiviral state. Because of its integral role in immune signaling, RIG-I activity must be precisely controlled. Recent studies have shown that RIG-I CARD-dependent signaling function is regulated by the dynamic balance between phosphorylation and TRIM25-induced K₆₃-linked ubiquitination. While ubiquitination of RIG-I is critical for RIG-I's ability to induce an antiviral IFN response, phosphorylation of RIG-I at S₈ or T₁₇₀ suppresses RIG-I signal-transducing activity under normal conditions. Here, we not only further define the roles of S₈ and T₁₇₀ phosphorylation for controlling RIG-I activity but also identify conventional protein kinase C-α (PKC-α) and PKC-β as important negative regulators of the RIG-I signaling pathway. Mutational analysis indicated that while the phosphorylation of S₈ or T₁₇₀ potently inhibits RIG-I downstream signaling, the dephosphorylation of RIG-I at both residues is necessary for optimal TRIM25 binding and ubiquitination-mediated RIG-I activation. Furthermore, exogenous expression, gene silencing, and specific inhibitor treatment demonstrated that PKC-α/β are the primary kinases responsible for RIG-I S₈ and T₁₇₀ phosphorylation. Coimmunoprecipitation showed that PKC-α/β interact with RIG-I under normal conditions, leading to its phosphorylation, which suppresses TRIM25 binding, RIG-I CARD ubiquitination, and thereby RIG-I-mediated IFN induction. PKC-α/β double-knockdown cells exhibited markedly decreased S₈/T₁₇₀ phosphorylation levels of RIG-I and resistance to infection by vesicular stomatitis virus. Thus, these findings demonstrate that PKC-α/β-induced RIG-I phosphorylation is a critical regulatory mechanism for controlling RIG-I antiviral signal transduction under normal conditions.

Conventional Protein Kinase C-α (PKC-α) and PKC-β Negatively Regulate RIG-I Antiviral Signal Transduction

PubMed Central

Maharaj, Natalya P.; Wies, Effi; Stoll, Andrej

2012-01-01

Retinoic acid-inducible gene I (RIG-I) is a key sensor for viral RNA in the cytosol, and it initiates a signaling cascade that leads to the establishment of an interferon (IFN)-mediated antiviral state. Because of its integral role in immune signaling, RIG-I activity must be precisely controlled. Recent studies have shown that RIG-I CARD-dependent signaling function is regulated by the dynamic balance between phosphorylation and TRIM25-induced K63-linked ubiquitination. While ubiquitination of RIG-I is critical for RIG-I's ability to induce an antiviral IFN response, phosphorylation of RIG-I at S8 or T170 suppresses RIG-I signal-transducing activity under normal conditions. Here, we not only further define the roles of S8 and T170 phosphorylation for controlling RIG-I activity but also identify conventional protein kinase C-α (PKC-α) and PKC-β as important negative regulators of the RIG-I signaling pathway. Mutational analysis indicated that while the phosphorylation of S8 or T170 potently inhibits RIG-I downstream signaling, the dephosphorylation of RIG-I at both residues is necessary for optimal TRIM25 binding and ubiquitination-mediated RIG-I activation. Furthermore, exogenous expression, gene silencing, and specific inhibitor treatment demonstrated that PKC-α/β are the primary kinases responsible for RIG-I S8 and T170 phosphorylation. Coimmunoprecipitation showed that PKC-α/β interact with RIG-I under normal conditions, leading to its phosphorylation, which suppresses TRIM25 binding, RIG-I CARD ubiquitination, and thereby RIG-I-mediated IFN induction. PKC-α/β double-knockdown cells exhibited markedly decreased S8/T170 phosphorylation levels of RIG-I and resistance to infection by vesicular stomatitis virus. Thus, these findings demonstrate that PKC-α/β-induced RIG-I phosphorylation is a critical regulatory mechanism for controlling RIG-I antiviral signal transduction under normal conditions. PMID:22114345

SSME HPFTP/AT Turbine Blade Platform Featherseal Damper Design

NASA Technical Reports Server (NTRS)

Montgomery, S. K.

1999-01-01

During the Space Shuttle Main Engines (SSM) HPFtP/AT development program, engine hot fire testing resulted in turbine blade fatigue cracks. The cracks were noted after only a few tests and a several hundred seconds versus the design goal of 60 tests and >30,000 seconds. Subsequent investigation attributed the distress to excessive steady and dynamic loads. To address these excessive turbine blade loads, Pratt & Whitney Liquid Space Propulsion engineers designed and developed retrofitable turbine blade to blade platform featherseal dampers. Since incorporation of these dampers, along with other turbine blade system improvements, there has been no observed SSME HPFTP/AT turbine blade fatigue cracking. The high time HPFTP/AT blade now has accumulated 32 starts and 19,200 seconds hot fire test time. Figure #1 illustrates the HPFTP/AT turbine blade platform featherseal dampers. The approached selected was to improve the turbine blade structural capability while simultaneously reducing loads. To achieve this goal, the featherseal dampers were designed to seal the blade to blade platform gap and damp the dynamic motions. Sealing improves the steady stress margins by increasing turbine efficiency and improving turbine blade attachment thermal conditioning. Load reduction was achieved through damping. Thin Haynes 188 sheet metal was selected based on its material properties (hydrogen resistance, elongation, tensile strengths, etc.). The 36,000 rpm wheel speed of the rotor result in a normal load of 120#/blade. The featherseals then act as micro-slip dampers during actual SSME operation. After initial design and analysis (prior to full engine testing), the featherseal dampers were tested in P&W's spin rig facility in West Palm Beach, Florida. Both dynamic strain gages and turbine blade tip displacement measurements were utilized to quantify the featherseal damper effectiveness. Full speed (36,000 rpm), room temperature rig testing verified the elimination of fundamental mode (i.e, modes 1 & 2) resonant response. The reduction in turbine blade dynamic response is shown for a typical turbine blade. This paper discusses the design and verification of these dampers. The numerous benefits associated with this design concept warrants consideration in existing and future turbomachinery applications.

Rod-cone based color vision in seals under photopic conditions.

PubMed

Oppermann, Daniela; Schramme, Jürgen; Neumeyer, Christa

2016-08-01

Marine mammals have lost the ability to express S-cone opsin, and possess only one type of M/L-cone in addition to numerous rods. As they are cone monochromats they should be color blind. However, early behavioral experiments with fur seals and sea lions indicated discrimination ability between many shades of grey and blue or green. On the other hand, most recent training experiments with harbor seals under "mesopic" conditions demonstrated rod based color blindness (Scholtyssek et al., 2015). In our experiments we trained two harbor seals (Phoca vitulina) and two South African fur seals (Arctocephalus pusillus) with surface colors under photopic conditions. The seals had to detect a triangle on grey background shown on one of three test fields while the other two test fields were homogeneously grey. In a first series of experiments we determined brightness detection. We found a luminance contrast of >3% sufficient for correctly choosing the triangle. In the tests for color vision the triangle was blue, green or yellow in grey surround. The results show that the animals could see the colored triangle despite minimal or zero brightness contrast. Thus, seals have color vision based on the contribution of cones and rods even in bright daylight. Copyright © 2016 Elsevier Ltd. All rights reserved.

Viral Pseudo Enzymes Activate RIG-I via Deamidation to Evade Cytokine Production

PubMed Central

He, Shanping; Zhao, Jun; Song, Shanshan; He, Xiaojing; Minassian, Arlet; Zhou, Yu; Zhang, Junjie; Brulois, Kevin; Wang, Yuqi; Cabo, Jackson; Zandi, Ebrahim; Liang, Chengyu; Jung, Jae U; Zhang, Xuewu; Feng, Pinghui

2015-01-01

SUMMARY RIG-I is a pattern recognition receptor that senses viral RNA and is crucial for host innate immune defense. Here we describe a mechanism of RIG-I activation through amidotransferase-mediated deamidation. We show that viral homologues of phosphoribosylformyglycinamide synthase (PFAS), although lacking intrinsic enzyme activity, recruit cellular PFAS to deamidate and activate RIG-I. Accordingly, depletion and biochemical inhibition of PFAS impair RIG-I deamidation and concomitant activation. Purified PFAS and viral homologue thereof deamidate RIG-I in vitro. Ultimately, herpesvirus hijacks activated RIG-I to avoid antiviral cytokine production; loss of RIG-I or inhibition of RIG-I deamidation results in elevated cytokine production. Together, these findings demonstrate a surprising mechanism of RIG-I activation that is mediated by an enzyme. PMID:25752576

Viral pseudo-enzymes activate RIG-I via deamidation to evade cytokine production.

PubMed

He, Shanping; Zhao, Jun; Song, Shanshan; He, Xiaojing; Minassian, Arlet; Zhou, Yu; Zhang, Junjie; Brulois, Kevin; Wang, Yuqi; Cabo, Jackson; Zandi, Ebrahim; Liang, Chengyu; Jung, Jae U; Zhang, Xuewu; Feng, Pinghui

2015-04-02

RIG-I is a pattern recognition receptor that senses viral RNA and is crucial for host innate immune defense. Here, we describe a mechanism of RIG-I activation through amidotransferase-mediated deamidation. We show that viral homologs of phosphoribosylformylglycinamidine synthetase (PFAS), although lacking intrinsic enzyme activity, recruit cellular PFAS to deamidate and activate RIG-I. Accordingly, depletion and biochemical inhibition of PFAS impair RIG-I deamidation and concomitant activation. Purified PFAS and viral homolog thereof deamidate RIG-I in vitro. Ultimately, herpesvirus hijacks activated RIG-I to avoid antiviral cytokine production; loss of RIG-I or inhibition of RIG-I deamidation results in elevated cytokine production. Together, these findings demonstrate a surprising mechanism of RIG-I activation that is mediated by an enzyme. Copyright © 2015 Elsevier Inc. All rights reserved.

An in situ tensile tester for studying electrochemical repassivation behavior: Fabrication and challenges

NASA Astrophysics Data System (ADS)

Neelakantan, Lakshman; Schönberger, Bernd; Eggeler, Gunther; Hassel, Achim Walter

2010-03-01

An in situ tensile rig is proposed, which allows performing electrochemical (repassivation) experiments during dynamic mechanical testing of wires. Utilizing the basic components of a conventional tensile tester, a custom-made minitensile rig was designed and fabricated. The maximal force that can be measured by the force sensor is 80 N, with a sensitivity of 0.5 mV/V. The maximum travel range of the crosshead induced by the motor is 10 mm with a minimum step size of 0.5 nm. The functionality of the tensile test rig was validated by investigating Cu and shape memory NiTi wires. Wires of lengths between 40 and 50 mm with varying gauge lengths can be tested. An interface between wire and electrochemical setup (noncontact) with a smart arrangement of electrodes facilitated the electrochemical measurements during tensile loading. Preliminary results on the repassivation behavior of Al wire are reported.

High Pressure Coolant Injection (HPCI) System Risk-Based Inspection Guide for Browns Ferry Nuclear Power Station

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wong, S.; DiBiasio, A.; Gunther, W.

1993-09-01

The High Pressure Coolant Injection (HPCI) system has been examined from a risk perspective. A System Risk-Based Inspection Guide (S-RIG) has been developed as an aid to HPCI system inspections at the Browns Ferry Nuclear Power Plant, Units 1, 2 and 3. The role of. the HPCI system in mitigating accidents is discussed in this S-RIG, along with insights on identified risk-based failure modes which could prevent proper operation of the system. The S-RIG provides a review of industry-wide operating experience, including plant-specific illustrative examples to augment the PRA and operational considerations in identifying a catalogue of basic PRA failuremore » modes for the HPCI system. It is designed to be used as a reference for routine inspections, self-initiated safety system functional inspections (SSFIs), and the evaluation of risk significance of component failures at the nuclear power plant.« less

Roles of RIG-I N-terminal tandem CARD and splice variant in TRIM25-mediated antiviral signal transduction

PubMed Central

Gack, Michaela U.; Kirchhofer, Axel; Shin, Young C.; Inn, Kyung-Soo; Liang, Chengyu; Cui, Sheng; Myong, Sua; Ha, Taekjip; Hopfner, Karl-Peter; Jung, Jae U.

2008-01-01

The caspase recruitment domain (CARD) of intracellular adaptors and sensors plays a critical role in the assembly of signaling complexes involved in innate host defense against pathogens and in the regulation of inflammatory responses. The cytosolic receptor retinoic acid-inducible gene-I (RIG-I) recognizes viral RNA in a 5′-triphosphate-dependent manner and initiates an antiviral signaling cascade. Upon viral infection, the N-terminal CARDs of RIG-I undergo the K63-linked ubiquitination induced by tripartite motif protein 25 (TRIM25), critical for the interaction of RIG-I with its downstream signaling partner MAVS/VISA/IPS-1/Cardif. Here, we demonstrate the distinct roles of RIG-I first and second CARD in TRIM25-mediated RIG-I ubiquitination: TRIM25 binds the RIG-I first CARD and subsequently ubiquitinates its second CARD. The T55I mutation in RIG-I first CARD abolishes TRIM25 interaction, whereas the K172R mutation in the second CARD eliminates polyubiquitin attachment. The necessity of the intact tandem CARD for RIG-I function is further evidenced by a RIG-I splice variant (SV) whose expression is robustly up-regulated upon viral infection. The RIG-I SV carries a short deletion (amino acids 36–80) within the first CARD and thereby loses TRIM25 binding, CARD ubiquitination, and downstream signaling ability. Furthermore, because of its robust inhibition of virus-induced RIG-I multimerization and RIG-I-MAVS signaling complex formation, this SV effectively suppresses the RIG-I-mediated IFN-β production. This study not only elucidates the vital role of the intact tandem CARD for TRIM25-mediated RIG-I activation but also identifies the RIG-I SV as an off-switch regulator of its own signaling pathway. PMID:18948594

Roles of RIG-I N-terminal tandem CARD and splice variant in TRIM25-mediated antiviral signal transduction.

PubMed

Gack, Michaela U; Kirchhofer, Axel; Shin, Young C; Inn, Kyung-Soo; Liang, Chengyu; Cui, Sheng; Myong, Sua; Ha, Taekjip; Hopfner, Karl-Peter; Jung, Jae U

2008-10-28

The caspase recruitment domain (CARD) of intracellular adaptors and sensors plays a critical role in the assembly of signaling complexes involved in innate host defense against pathogens and in the regulation of inflammatory responses. The cytosolic receptor retinoic acid-inducible gene-I (RIG-I) recognizes viral RNA in a 5'-triphosphate-dependent manner and initiates an antiviral signaling cascade. Upon viral infection, the N-terminal CARDs of RIG-I undergo the K(63)-linked ubiquitination induced by tripartite motif protein 25 (TRIM25), critical for the interaction of RIG-I with its downstream signaling partner MAVS/VISA/IPS-1/Cardif. Here, we demonstrate the distinct roles of RIG-I first and second CARD in TRIM25-mediated RIG-I ubiquitination: TRIM25 binds the RIG-I first CARD and subsequently ubiquitinates its second CARD. The T(55)I mutation in RIG-I first CARD abolishes TRIM25 interaction, whereas the K(172)R mutation in the second CARD eliminates polyubiquitin attachment. The necessity of the intact tandem CARD for RIG-I function is further evidenced by a RIG-I splice variant (SV) whose expression is robustly up-regulated upon viral infection. The RIG-I SV carries a short deletion (amino acids 36-80) within the first CARD and thereby loses TRIM25 binding, CARD ubiquitination, and downstream signaling ability. Furthermore, because of its robust inhibition of virus-induced RIG-I multimerization and RIG-I-MAVS signaling complex formation, this SV effectively suppresses the RIG-I-mediated IFN-beta production. This study not only elucidates the vital role of the intact tandem CARD for TRIM25-mediated RIG-I activation but also identifies the RIG-I SV as an off-switch regulator of its own signaling pathway.

Minimum area rig concept update: H and P 101 modifications and first infield move

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sigurdson, S.R.

1987-03-01

The minimum area rig concept (MARC) is a cost-effective alternative to the typical self-contained platform rig (SCPR). Helmerich and Payne (HandP) built the first MARC rig, HandP 101, to drill and to work over wells up to 16,000 ft (4877 m) measured depth. This rig began operation in May 1983 in the Gulf of Mexico at Arco Oil and Gas Co.'s South Pass Block 61 field and has undergone one infield move. Since the rig's initial mobilization, several rig modifications have been added to increase storage area, to promote safety, to provide a more efficient drilling/workover rig, and to reducemore » overall move time. This paper describes the modifications and recaps the rig's first move. This provides further insight into the MARC rig and show the benefits of the MARC design in relation to a move.« less

Extraterrestrial surface propulsion systems

NASA Astrophysics Data System (ADS)

Ash, Robert L.; Blackstock, Dexter L.; Barnhouse, K.; Charalambous, Z.; Coats, J.; Danagan, J.; Davis, T.; Dickens, J.; Harris, P.; Horner, G.

Lunar traction systems, Mars oxygen production, and Mars methane engine operation were the three topics studied during 1992. An elastic loop track system for lunar construction operations was redesigned and is being tested. A great deal of work on simulating the lunar environment to facilitate traction testing has been reported. Operation of an oxygen processor under vacuum conditions has been the focus of another design team. They have redesigned the processor facility. This included improved seals and heat shields. Assuming methane and oxygen can be produced from surface resources on Mars, a third design team has addressed the problem of using Mars atmospheric carbon dioxide to control combustion temperatures in an internal combustion engine. That team has identified appropriate tests and instrumentation. They have reported on the test rig that they designed and the computer-based system for acquiring data.

Extraterrestrial surface propulsion systems

NASA Technical Reports Server (NTRS)

Ash, Robert L.; Blackstock, Dexter L.; Barnhouse, K.; Charalambous, Z.; Coats, J.; Danagan, J.; Davis, T.; Dickens, J.; Harris, P.; Horner, G.

1992-01-01

Lunar traction systems, Mars oxygen production, and Mars methane engine operation were the three topics studied during 1992. An elastic loop track system for lunar construction operations was redesigned and is being tested. A great deal of work on simulating the lunar environment to facilitate traction testing has been reported. Operation of an oxygen processor under vacuum conditions has been the focus of another design team. They have redesigned the processor facility. This included improved seals and heat shields. Assuming methane and oxygen can be produced from surface resources on Mars, a third design team has addressed the problem of using Mars atmospheric carbon dioxide to control combustion temperatures in an internal combustion engine. That team has identified appropriate tests and instrumentation. They have reported on the test rig that they designed and the computer-based system for acquiring data.

Paramyxovirus V protein interaction with the antiviral sensor LGP2 disrupts MDA5 signaling enhancement but is not relevant to LGP2-mediated RLR signaling inhibition.

PubMed

Rodriguez, Kenny R; Horvath, Curt M

2014-07-01

The interferon antiviral system is a primary barrier to virus replication triggered upon recognition of nonself RNAs by the cytoplasmic sensors encoded by retinoic acid-inducible gene I (RIG-I), melanoma differentiation-associated gene 5 (MDA5), and laboratory of genetics and physiology gene 2 (LGP2). Paramyxovirus V proteins are interferon antagonists that can selectively interact with MDA5 and LGP2 through contact with a discrete helicase domain region. Interaction with MDA5, an activator of antiviral signaling, disrupts interferon gene expression and antiviral responses. LGP2 has more diverse reported roles as both a coactivator of MDA5 and a negative regulator of both RIG-I and MDA5. This functional dichotomy, along with the concurrent interference with both cellular targets, has made it difficult to assess the unique consequences of V protein interaction with LGP2. To directly evaluate the impact of LGP2 interference, MDA5 and LGP2 variants unable to be recognized by measles virus and parainfluenza virus 5 (PIV5) V proteins were tested in signaling assays. Results indicate that interaction with LGP2 specifically prevents coactivation of MDA5 signaling and that LGP2's negative regulatory capacity was not affected. V proteins only partially antagonize RIG-I at high concentrations, and their expression had no additive effects on LGP2-mediated negative regulation. However, conversion of RIG-I to a direct V protein target was accomplished by only two amino acid substitutions that allowed both V protein interaction and efficient interference. These results clarify the unique consequences of MDA5 and LGP2 interference by paramyxovirus V proteins and help resolve the distinct roles of LGP2 in both activation and inhibition of antiviral signal transduction. Importance: Paramyxovirus V proteins interact with two innate immune receptors, MDA5 and LGP2, but not RIG-I. V proteins prevent MDA5 from signaling to the beta interferon promoter, but the consequences of LGP2 targeting are poorly understood. As the V protein targets MDA5 and LGP2 simultaneously, and LGP2 is both a positive and negative regulator of both MDA5 and RIG-I, it has been difficult to evaluate the specific advantages conferred by LGP2 targeting. Experiments with V-insensitive proteins revealed that the primary outcome of LGP2 interference is suppression of its ability to synergize with MDA5. LGP2's negative regulation of MDA5 and RIG-I remains intact irrespective of V protein interaction. Complementary experiments demonstrate that RIG-I can be converted to V protein sensitivity by two amino acid substitutions. These findings clarify the functions of LGP2 as a positive regulator of MDA5 signaling, demonstrate the basis for V-mediated LGP2 targeting, and broaden our understanding of paramyxovirus-host interactions. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Paramyxovirus V Protein Interaction with the Antiviral Sensor LGP2 Disrupts MDA5 Signaling Enhancement but Is Not Relevant to LGP2-Mediated RLR Signaling Inhibition

PubMed Central

Rodriguez, Kenny R.

2014-01-01

ABSTRACT The interferon antiviral system is a primary barrier to virus replication triggered upon recognition of nonself RNAs by the cytoplasmic sensors encoded by retinoic acid-inducible gene I (RIG-I), melanoma differentiation-associated gene 5 (MDA5), and laboratory of genetics and physiology gene 2 (LGP2). Paramyxovirus V proteins are interferon antagonists that can selectively interact with MDA5 and LGP2 through contact with a discrete helicase domain region. Interaction with MDA5, an activator of antiviral signaling, disrupts interferon gene expression and antiviral responses. LGP2 has more diverse reported roles as both a coactivator of MDA5 and a negative regulator of both RIG-I and MDA5. This functional dichotomy, along with the concurrent interference with both cellular targets, has made it difficult to assess the unique consequences of V protein interaction with LGP2. To directly evaluate the impact of LGP2 interference, MDA5 and LGP2 variants unable to be recognized by measles virus and parainfluenza virus 5 (PIV5) V proteins were tested in signaling assays. Results indicate that interaction with LGP2 specifically prevents coactivation of MDA5 signaling and that LGP2's negative regulatory capacity was not affected. V proteins only partially antagonize RIG-I at high concentrations, and their expression had no additive effects on LGP2-mediated negative regulation. However, conversion of RIG-I to a direct V protein target was accomplished by only two amino acid substitutions that allowed both V protein interaction and efficient interference. These results clarify the unique consequences of MDA5 and LGP2 interference by paramyxovirus V proteins and help resolve the distinct roles of LGP2 in both activation and inhibition of antiviral signal transduction. IMPORTANCE Paramyxovirus V proteins interact with two innate immune receptors, MDA5 and LGP2, but not RIG-I. V proteins prevent MDA5 from signaling to the beta interferon promoter, but the consequences of LGP2 targeting are poorly understood. As the V protein targets MDA5 and LGP2 simultaneously, and LGP2 is both a positive and negative regulator of both MDA5 and RIG-I, it has been difficult to evaluate the specific advantages conferred by LGP2 targeting. Experiments with V-insensitive proteins revealed that the primary outcome of LGP2 interference is suppression of its ability to synergize with MDA5. LGP2's negative regulation of MDA5 and RIG-I remains intact irrespective of V protein interaction. Complementary experiments demonstrate that RIG-I can be converted to V protein sensitivity by two amino acid substitutions. These findings clarify the functions of LGP2 as a positive regulator of MDA5 signaling, demonstrate the basis for V-mediated LGP2 targeting, and broaden our understanding of paramyxovirus-host interactions. PMID:24829334

Structural and biochemical studies of RIG-I antiviral signaling.

PubMed

Feng, Miao; Ding, Zhanyu; Xu, Liang; Kong, Liangliang; Wang, Wenjia; Jiao, Shi; Shi, Zhubing; Greene, Mark I; Cong, Yao; Zhou, Zhaocai

2013-02-01

Retinoic acid-inducible gene I (RIG-I) is an important pattern recognition receptor that detects viral RNA and triggers the production of type-I interferons through the downstream adaptor MAVS (also called IPS-1, CARDIF, or VISA). A series of structural studies have elaborated some of the mechanisms of dsRNA recognition and activation of RIG-I. Recent studies have proposed that K63-linked ubiquitination of, or unanchored K63-linked polyubiquitin binding to RIG-I positively regulates MAVS-mediated antiviral signaling. Conversely phosphorylation of RIG-I appears to play an inhibitory role in controlling RIG-I antiviral signal transduction. Here we performed a combined structural and biochemical study to further define the regulatory features of RIG-I signaling. ATP and dsRNA binding triggered dimerization of RIG-I with conformational rearrangements of the tandem CARD domains. Full length RIG-I appeared to form a complex with dsRNA in a 2:2 molar ratio. Compared with the previously reported crystal structures of RIG-I in inactive state, our electron microscopic structure of full length RIG-I in complex with blunt-ended dsRNA, for the first time, revealed an exposed active conformation of the CARD domains. Moreover, we found that purified recombinant RIG-I proteins could bind to the CARD domain of MAVS independently of dsRNA, while S8E and T170E phosphorylation-mimicking mutants of RIG-I were defective in binding E3 ligase TRIM25, unanchored K63-linked polyubiquitin, and MAVS regardless of dsRNA. These findings suggested that phosphorylation of RIG inhibited downstream signaling by impairing RIG-I binding with polyubiquitin and its interaction with MAVS.

Negative role of RIG-I serine 8 phosphorylation in the regulation of interferon-beta production.

PubMed

Nistal-Villán, Estanislao; Gack, Michaela U; Martínez-Delgado, Gustavo; Maharaj, Natalya P; Inn, Kyung-Soo; Yang, Heyi; Wang, Rong; Aggarwal, Aneel K; Jung, Jae U; García-Sastre, Adolfo

2010-06-25

RIG-I (retinoic acid-inducible gene I) and TRIM25 (tripartite motif protein 25) have emerged as key regulatory factors to induce interferon (IFN)-mediated innate immune responses to limit viral replication. Upon recognition of viral RNA, TRIM25 E3 ligase binds the first caspase recruitment domain (CARD) of RIG-I and subsequently induces lysine 172 ubiquitination of the second CARD of RIG-I, which is essential for the interaction with downstream MAVS/IPS-1/CARDIF/VISA and, thereby, IFN-beta mRNA production. Although ubiquitination has emerged as a major factor involved in RIG-I activation, the potential contribution of other post-translational modifications, such as phosphorylation, to the regulation of RIG-I activity has not been addressed. Here, we report the identification of serine 8 phosphorylation at the first CARD of RIG-I as a negative regulatory mechanism of RIG-I-mediated IFN-beta production. Immunoblot analysis with a phosphospecific antibody showed that RIG-I serine 8 phosphorylation steady-state levels were decreased upon stimulation of cells with IFN-beta or virus infection. Substitution of serine 8 in the CARD RIG-I functional domain with phosphomimetic aspartate or glutamate results in decreased TRIM25 binding, RIG-I ubiquitination, MAVS binding, and downstream signaling. Finally, sequence comparison reveals that only primate species carry serine 8, whereas other animal species carry an asparagine, indicating that serine 8 phosphorylation may represent a primate-specific regulation of RIG-I activation. Collectively, these data suggest that the phosphorylation of RIG-I serine 8 operates as a negative switch of RIG-I activation by suppressing TRIM25 interaction, further underscoring the importance of RIG-I and TRIM25 connection in type I IFN signal transduction.

Theoretical analysis and experimental study on breakaway torque of large-diameter magnetic liquid seal at low temperature

NASA Astrophysics Data System (ADS)

Zhang, Haina; Li, Decai; Wang, Qinglei; Zhang, Zhili

2013-07-01

The existing researches of the magnetic liquid rotation seal have been mainly oriented to the seal at normal temperature and the seal with the smaller shaft diameter less than 100 mm. However, the large-diameter magnetic liquid rotation seal at low temperature has not been reported both in theory and in application up to now. A key factor restricting the application of the large-diameter magnetic liquid rotation seal at low temperature is the high breakaway torque. In this paper, the factors that influence the breakaway torque including the number of seal stages, the injected quantity of magnetic liquid and the standing time at normal temperature are studied. Two kinds of magnetic liquid with variable content of large particles are prepared first, and a seal feedthrough with 140 mm shaft diameter is used in the experiments. All experiments are carried out in a low temperature chamber with a temperature range from 200°C to -100°C. Different numbers of seal stages are tested under the same condition to study the relation between the breakaway torque and the number of seal stages. Variable quantity of magnetic liquid is injected in the seal gap to get the relation curve of the breakaway torque and the injecting quantity of magnetic liquid. In the experiment for studying the relation between the breakaway torque and the standing time at the normal temperature, the seal feedtrough is laid at normal temperature for different period of time before it is put in the low temperature chamber. The experimental results show that the breakaway torque is proportional to the number of seal stages, the injected quantity of magnetic liquid and the standing time at the normal temperature. Meanwhile, the experimental results are analyzed and the torque formula of magnetic liquid rotation seal at low temperature is deduced from the Navier-Stokes equation on the base of the model of magnetic liquid rotation seal. The presented research can make wider application of the magnetic liquid seal in general. And the large-diameter magnetic liquid rotation seal at low temperature designed by using present research results are to be used in some special fields, such as the military field, etc.

The Full Scale Seal Experiment - A Seal Industrial Prototype for Cigeo - 13106

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lebon, P.; Bosgiraud, J.M.; Foin, R.

2013-07-01

The Full Scale Seal (FSS) Experiment is one of various experiments implemented by Andra, within the frame of the Cigeo (the French Deep Geological Repository) Project development, to demonstrate the technical construction feasibility and performance of seals to be constructed, at time of Repository components (shafts, ramps, drifts, disposal vaults) progressive closure. FSS is built inside a drift model fabricated on surface for the purpose. Prior to the scale 1:1 seal construction test, various design tasks are scheduled. They include the engineering work on the drift model to make it fit with the experimental needs, on the various work sequencesmore » anticipated for the swelling clay core emplacement and the concrete containment plugs construction, on the specialized handling tools (and installation equipment) manufactured and delivered for the purpose, and of course on the various swelling clay materials and low pH (below 11) concrete formulations developed for the application. The engineering of the 'seal-as-built' commissioning means (tools and methodology) must also be dealt with. The FSS construction experiment is a technological demonstrator, thus it is not focused on the phenomenological survey (and by consequence, on the performance and behaviour forecast). As such, no hydration (forced or natural) is planned. However, the FSS implementation (in particular via the construction and commissioning activities carried out) is a key milestone in view of comforting phenomenological extrapolation in time and scale. The FSS experiment also allows for qualifying the commissioning methods of a real sealing system in the Repository, as built, at time of industrial operations. (authors)« less

Characterization and durability testing of plasma-sprayed zirconia-yttria and hafnia-yttria thermal barrier coatings. Part 1: Effect of spray parameters on the performance of several lots of partially stabilized zirconia-yttria powder

NASA Technical Reports Server (NTRS)

Miller, Robert A.; Leissler, George W.; Jobe, J. Marcus

1993-01-01

Initial experiments conducted on thermal barrier coatings prepared in the newly upgraded research plasma spray facility and the burner rig test facilities are discussed. Part 1 discusses experiments which establish the spray parameters for three baseline zirconia-yttria coatings. The quality of five similar coating lots was judged primarily by their response to burner rig exposure supplemented by data from other sources such as specimen characterizations and thermal diffusivity measurements. After allowing for burner rig variability, although there appears to be an optimum density (i.e., optimum microstructure) for maximum burner rig life, the distribution tends to be rather broad about the maximum. In Part 2, new hafnia-yttria-based coatings were evaluated against both baseline and alternate zirconia-yttria coatings. The hafnia-yttria coatings and the zirconia-yttria coatings that were prepared by an alternate powder vendor were very sensitive to plasma spray parameters, in that high-quality coatings were only obtained when certain parameters were employed. The reasons for this important observation are not understood. Also not understood is that the first of two replicate specimens sprayed for Part 1 consistently performed better than the second specimen. Subsequent experiments did not display this spray order affect, possibly because a chiller was installed in the torch cooling water circuit. Also, large changes in coating density were observed after switching to a new lot of electrodes. Analyses of these findings were made possible, in part, because of the development of a sensitive density measurement technique described herein in detail. The measured thermal diffusivities did not display the expected strong relationship with porosity. This surprising result was believed to have been caused by increased microcracking of the denser coatings on the stainless steel substrates.

Some Numerical Simulations and an Experimental Investigation of Finger Seals

NASA Technical Reports Server (NTRS)

Braun, Minel J.; Smith, Ian; Marie, Hazel

2007-01-01

All seal types have been shown to lift effectively, and experience only minor wear during startup. .. The double pad design outperforms previous seals, providing lower operating temperatures, and less leakage at higher pressures. .. Future experimentation at higher pressures, temperatures, and operating speeds will show the full potential of finger sealing technology.

Why do seals have cones? Behavioural evidence for colour-blindness in harbour seals.

PubMed

Scholtyssek, Christine; Kelber, Almut; Dehnhardt, Guido

2015-03-01

All seals and cetaceans have lost at least one of two ancestral cone classes and should therefore be colour-blind. Nevertheless, earlier studies showed that these marine mammals can discriminate colours and a colour vision mechanism has been proposed which contrasts signals from cones and rods. However, these earlier studies underestimated the brightness discrimination abilities of these animals, so that they could have discriminated colours using brightness only. Using a psychophysical discrimination experiment, we showed that a harbour seal can solve a colour discrimination task by means of brightness discrimination alone. Performing a series of experiments in which two harbour seals had to discriminate the brightness of colours, we also found strong evidence for purely scotopic (rod-based) vision at light levels that lead to mesopic (rod-cone-based) vision in other mammals. This finding speaks against rod-cone-based colour vision in harbour seals. To test for colour-blindness, we used a cognitive approach involving a harbour seal trained to use a concept of same and different. We tested this seal with pairs of isoluminant stimuli that were either same or different in colour. If the seal had perceived colour, it would have responded to colour differences between stimuli. However, the seal responded with "same", providing strong evidence for colour-blindness.

Riplet/RNF135, a RING finger protein, ubiquitinates RIG-I to promote interferon-beta induction during the early phase of viral infection.

PubMed

Oshiumi, Hiroyuki; Matsumoto, Misako; Hatakeyama, Shigetsugu; Seya, Tsukasa

2009-01-09

RIG-I (retinoic acid-inducible gene-I), a cytoplasmic RNA helicase, interacts with IPS-1/MAVS/Cardif/VISA, a protein on the outer membrane of mitochondria, to signal the presence of virus-derived RNA and induce type I interferon production. Activation of RIG-I requires the ubiquitin ligase, TRIM25, which mediates lysine 63-linked polyubiquitination of the RIG-I N-terminal CARD-like region. However, how this modification proceeds for activation of IPS-1 by RIG-I remains unclear. Here we identify an alternative factor, Riplet/RNF135, that promotes RIG-I activation independent of TRIM25. The Riplet/RNF135 protein consists of an N-terminal RING finger domain, C-terminal SPRY and PRY motifs, and shows sequence similarity to TRIM25. Immunoprecipitation analyses demonstrated that the C-terminal helicase and repressor domains of RIG-I interact with the Riplet/RNF135 C-terminal region, whereas the CARD-like region of RIG-I is dispensable for this interaction. Riplet/RNF135 promotes lysine 63-linked polyubiquitination of the C-terminal region of RIG-I, modification of which differs from the N-terminal ubiquitination by TRIM25. Overexpression and knockdown analyses revealed that Riplet/RNF135 promotes RIG-I-mediated interferon-beta promoter activation and inhibits propagation of the negative-strand RNA virus, vesicular stomatitis virus. Our data suggest that Riplet/RNF135 is a novel factor of the RIG-I pathway that is involved in the evoking of human innate immunity against RNA virus infection, and activates RIG-I through ubiquitination of its C-terminal region. We infer that a variety of RIG-I-ubiquitinating molecular complexes sustain RIG-I activation to modulate RNA virus replication in the cytoplasm.

DOE Office of Scientific and Technical Information (OSTI.GOV)

E Nistal-Villan; M Gack; G Martinez-Delgado

RIG-I (retinoic acid-inducible gene I) and TRIM25 (tripartite motif protein 25) have emerged as key regulatory factors to induce interferon (IFN)-mediated innate immune responses to limit viral replication. Upon recognition of viral RNA, TRIM25 E3 ligase binds the first caspase recruitment domain (CARD) of RIG-I and subsequently induces lysine 172 ubiquitination of the second CARD of RIG-I, which is essential for the interaction with downstream MAVS/IPS-1/CARDIF/VISA and, thereby, IFN-beta mRNA production. Although ubiquitination has emerged as a major factor involved in RIG-I activation, the potential contribution of other post-translational modifications, such as phosphorylation, to the regulation of RIG-I activity hasmore » not been addressed. Here, we report the identification of serine 8 phosphorylation at the first CARD of RIG-I as a negative regulatory mechanism of RIG-I-mediated IFN-beta production. Immunoblot analysis with a phosphospecific antibody showed that RIG-I serine 8 phosphorylation steady-state levels were decreased upon stimulation of cells with IFN-beta or virus infection. Substitution of serine 8 in the CARD RIG-I functional domain with phosphomimetic aspartate or glutamate results in decreased TRIM25 binding, RIG-I ubiquitination, MAVS binding, and downstream signaling. Finally, sequence comparison reveals that only primate species carry serine 8, whereas other animal species carry an asparagine, indicating that serine 8 phosphorylation may represent a primate-specific regulation of RIG-I activation. Collectively, these data suggest that the phosphorylation of RIG-I serine 8 operates as a negative switch of RIG-I activation by suppressing TRIM25 interaction, further underscoring the importance of RIG-I and TRIM25 connection in type I IFN signal transduction.« less

Leak rate of seals: Effective-medium theory and comparison with experiment.

PubMed

Lorenz, B; Persson, B N J

2010-02-01

Seals are extremely useful devices to prevent fluid leakage. We present an effective-medium theory of the leak rate of rubber seals, which is based on a recently developed contact mechanics theory. We compare the theory with experimental results for seals consisting of silicon rubber in contact with sandpaper and sand-blasted PMMA surfaces.

The mitochondrial targeting chaperone 14-3-3ε regulates a RIG-I translocon that mediates membrane-association and innate antiviral immunity

PubMed Central

Liu, Helene Minyi; Loo, Yueh-Ming; Horner, Stacy M.; Zornetzer, Gregory A.; Katze, Michael G.; Gale, Michael

2012-01-01

Summary RIG-I is a cytosolic pathogen recognition receptor that initiates immune responses against RNA viruses. Upon viral RNA recognition, anti-viral signalling requires RIG-I redistribution from the cytosol to membranes where it binds the adaptor protein, MAVS. Here we identify the mitochondrial targeting chaperone protein, 14-3-3ε, as a RIG-I-binding partner and essential component of a translocation complex or “translocon” containing RIG-I, 14-3-3ε, and the TRIM25 ubiquitin ligase. The RIG-I translocon directs RIG-I redistribution from the cytosol to membranes where it mediates MAVS-dependent innate immune signalling during acute RNA virus infection. 14-3-3ε is essential for the stable interaction of RIG-I with TRIM25, which facilitates RIG-I ubiquitination and initiation of innate immunity against hepatitis C virus and other pathogenic RNA viruses. Our results define 14-3-3ε as a key component of a RIG-I translocon required for innate antiviral immunity. PMID:22607805

The mitochondrial targeting chaperone 14-3-3ε regulates a RIG-I translocon that mediates membrane association and innate antiviral immunity.

PubMed

Liu, Helene Minyi; Loo, Yueh-Ming; Horner, Stacy M; Zornetzer, Gregory A; Katze, Michael G; Gale, Michael

2012-05-17

RIG-I is a cytosolic pathogen recognition receptor that initiates immune responses against RNA viruses. Upon viral RNA recognition, antiviral signaling requires RIG-I redistribution from the cytosol to membranes where it binds the adaptor protein, MAVS. Here we identify the mitochondrial targeting chaperone protein, 14-3-3ε, as a RIG-I-binding partner and essential component of a translocation complex or "translocon" containing RIG-I, 14-3-3ε, and the TRIM25 ubiquitin ligase. The RIG-I translocon directs RIG-I redistribution from the cytosol to membranes where it mediates MAVS-dependent innate immune signaling during acute RNA virus infection. 14-3-3ε is essential for the stable interaction of RIG-I with TRIM25, which facilitates RIG-I ubiquitination and initiation of innate immunity against hepatitis C virus and other pathogenic RNA viruses. Our results define 14-3-3ε as a key component of a RIG-I translocon required for innate antiviral immunity. Copyright © 2012 Elsevier Inc. All rights reserved.

A distinct role of Riplet-mediated K63-Linked polyubiquitination of the RIG-I repressor domain in human antiviral innate immune responses.

PubMed

Oshiumi, Hiroyuki; Miyashita, Moeko; Matsumoto, Misako; Seya, Tsukasa

2013-01-01

The innate immune system is essential for controlling viral infections, but several viruses have evolved strategies to escape innate immunity. RIG-I is a cytoplasmic viral RNA sensor that triggers the signal to induce type I interferon production in response to viral infection. RIG-I activation is regulated by the K63-linked polyubiquitin chain mediated by Riplet and TRIM25 ubiquitin ligases. TRIM25 is required for RIG-I oligomerization and interaction with the IPS-1 adaptor molecule. A knockout study revealed that Riplet was essential for RIG-I activation. However the molecular mechanism underlying RIG-I activation by Riplet remains unclear, and the functional differences between Riplet and TRIM25 are also unknown. A genetic study and a pull-down assay indicated that Riplet was dispensable for RIG-I RNA binding activity but required for TRIM25 to activate RIG-I. Mutational analysis demonstrated that Lys-788 within the RIG-I repressor domain was critical for Riplet-mediated K63-linked polyubiquitination and that Riplet was required for the release of RIG-I autorepression of its N-terminal CARDs, which leads to the association of RIG-I with TRIM25 ubiquitin ligase and TBK1 protein kinase. Our data indicate that Riplet is a prerequisite for TRIM25 to activate RIG-I signaling. We investigated the biological importance of this mechanism in human cells and found that hepatitis C virus (HCV) abrogated this mechanism. Interestingly, HCV NS3-4A proteases targeted the Riplet protein and abrogated endogenous RIG-I polyubiquitination and association with TRIM25 and TBK1, emphasizing the biological importance of this mechanism in human antiviral innate immunity. In conclusion, our results establish that Riplet-mediated K63-linked polyubiquitination released RIG-I RD autorepression, which allowed the access of positive factors to the RIG-I protein.

A Distinct Role of Riplet-Mediated K63-Linked Polyubiquitination of the RIG-I Repressor Domain in Human Antiviral Innate Immune Responses

PubMed Central

Oshiumi, Hiroyuki; Miyashita, Moeko; Matsumoto, Misako; Seya, Tsukasa

2013-01-01

The innate immune system is essential for controlling viral infections, but several viruses have evolved strategies to escape innate immunity. RIG-I is a cytoplasmic viral RNA sensor that triggers the signal to induce type I interferon production in response to viral infection. RIG-I activation is regulated by the K63-linked polyubiquitin chain mediated by Riplet and TRIM25 ubiquitin ligases. TRIM25 is required for RIG-I oligomerization and interaction with the IPS-1 adaptor molecule. A knockout study revealed that Riplet was essential for RIG-I activation. However the molecular mechanism underlying RIG-I activation by Riplet remains unclear, and the functional differences between Riplet and TRIM25 are also unknown. A genetic study and a pull-down assay indicated that Riplet was dispensable for RIG-I RNA binding activity but required for TRIM25 to activate RIG-I. Mutational analysis demonstrated that Lys-788 within the RIG-I repressor domain was critical for Riplet-mediated K63-linked polyubiquitination and that Riplet was required for the release of RIG-I autorepression of its N-terminal CARDs, which leads to the association of RIG-I with TRIM25 ubiquitin ligase and TBK1 protein kinase. Our data indicate that Riplet is a prerequisite for TRIM25 to activate RIG-I signaling. We investigated the biological importance of this mechanism in human cells and found that hepatitis C virus (HCV) abrogated this mechanism. Interestingly, HCV NS3-4A proteases targeted the Riplet protein and abrogated endogenous RIG-I polyubiquitination and association with TRIM25 and TBK1, emphasizing the biological importance of this mechanism in human antiviral innate immunity. In conclusion, our results establish that Riplet-mediated K63-linked polyubiquitination released RIG-I RD autorepression, which allowed the access of positive factors to the RIG-I protein. PMID:23950712

Deep Boreholes Seals Subjected to High P,T conditions - Proposed Experimental Studies

NASA Astrophysics Data System (ADS)

Caporuscio, F.

2015-12-01

Deep borehole experimental work will constrain the P,T conditions which "seal" material will experience in deep borehole crystalline rock repositories. The rocks of interest to this study include mafic (amphibolites) and silicic (granitic gneiss) end members. The experiments will systematically add components to capture discrete changes in both water and EBS component chemistries. Experiments in the system wall rock-clay-concrete-groundwater will evaluate interactions among components, including: mineral phase stability, metal corrosion rates and thermal limits. Based on engineered barrier studies, experimental investigations will move forward with three focusses. First, evaluation of interaction between "seal" materials and repository wall rock (crystalline) under fluid-saturated conditions over long-term (i.e., six-month) experiments; which reproduces the thermal pulse event of a repository. Second, perform experiments to determine the stability of zeolite minerals (analcime-wairakitess) under repository conditions. Both sets of experiments are critically important for understanding mineral paragenesis (zeolites and/or clay transformations) associated with "seals" in contact with wall rock at elevated temperatures. Third, mineral growth at the metal interface is a principal control on the survivability (i.e. corrosion) of waste canisters in a repository. The objective of this planned experimental work is to evaluate physio-chemical processes for 'seal' components and materials relevant to deep borehole disposal. These evaluations will encompass multi-laboratory efforts for the development of seals concepts and application of Thermal-Mechanical-Chemical (TMC) modeling work to assess barrier material interactions with subsurface fluids and other barrier materials, their stability at high temperatures, and the implications of these processes to the evaluation of thermal limits.

Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.

PubMed

Liu, Wei; Li, Jing; Zheng, Weinan; Shang, Yingli; Zhao, Zhendong; Wang, Shanshan; Bi, Yuhai; Zhang, Shuang; Xu, Chongfeng; Duan, Ziyuan; Zhang, Lianfeng; Wang, Yue L; Jiang, Zhengfan; Liu, Wenjun; Sun, Lei

2017-06-08

RIG-I is a key cytosolic pattern recognition receptor that interacts with MAVS to induce type I interferons (IFNs) against RNA virus infection. In this study, we found that cyclophilin A (CypA), a peptidyl-prolyl cis/trans isomerase, functioned as a critical positive regulator of RIG-I-mediated antiviral immune responses. Deficiency of CypA impaired RIG-I-mediated type I IFN production and promoted viral replication in human cells and mice. Upon Sendai virus infection, CypA increased the interaction between RIG-I and its E3 ubiquitin ligase TRIM25, leading to enhanced TRIM25-mediated K63-linked ubiquitination of RIG-I that facilitated recruitment of RIG-I to MAVS. In addition, CypA and TRIM25 competitively interacted with MAVS, thereby inhibiting TRIM25-induced K48-linked ubiquitination of MAVS. Taken together, our findings reveal an essential role of CypA in boosting RIG-I-mediated antiviral immune responses by controlling the ubiquitination of RIG-I and MAVS.

Design and Analysis of Tooth Impact Test Rig for Spur Gear

NASA Astrophysics Data System (ADS)

Ghazali, Wafiuddin Bin Md; Aziz, Ismail Ali Bin Abdul; Daing Idris, Daing Mohamad Nafiz Bin; Ismail, Nurazima Binti; Sofian, Azizul Helmi Bin

2016-02-01

This paper is about the design and analysis of a prototype of tooth impact test rig for spur gear. The test rig was fabricated and analysis was conducted to study its’ limitation and capabilities. The design of the rig is analysed to ensure that there will be no problem occurring during the test and reliable data can be obtained. From the result of the analysis, the maximum amount of load that can be applied, the factor of safety of the machine, the stresses on the test rig parts were determined. This is important in the design consideration of the test rig. The materials used for the fabrication of the test rig were also discussed and analysed. MSC Nastran Patran software was used to analyse the model, which was designed by using SolidWorks 2014 software. Based from the results, there were limitations found from the initial design and the test rig design needs to be improved in order for the test rig to operate properly.

Foot-and-mouth disease virus non-structural protein 3A inhibits the interferon-β signaling pathway

PubMed Central

Li, Dan; Lei, Caoqi; Xu, Zhisheng; Yang, Fan; Liu, Huanan; Zhu, Zixiang; Li, Shu; Liu, Xiangtao; Shu, Hongbing; Zheng, Haixue

2016-01-01

Foot-and-mouth disease virus (FMDV) is the etiological agent of FMD, which affects cloven-hoofed animals. The pathophysiology of FMDV has not been fully understood and the evasion of host innate immune system is still unclear. Here, the FMDV non-structural protein 3A was identified as a negative regulator of virus-triggered IFN-β signaling pathway. Overexpression of the FMDV 3A inhibited Sendai virus-triggered activation of IRF3 and the expressions of RIG-I/MDA5. Transient transfection and co-immunoprecipitation experiments suggested that FMDV 3A interacts with RIG-I, MDA5 and VISA, which is dependent on the N-terminal 51 amino acids of 3A. Furthermore, 3A also inhibited the expressions of RIG-I, MDA5, and VISA by disrupting their mRNA levels. These results demonstrated that 3A inhibits the RLR-mediated IFN-β induction and uncovered a novel mechanism by which the FMDV 3A protein evades the host innate immune system. PMID:26883855

Calibration of stereo rigs based on the backward projection process

NASA Astrophysics Data System (ADS)

Gu, Feifei; Zhao, Hong; Ma, Yueyang; Bu, Penghui; Zhao, Zixin

2016-08-01

High-accuracy 3D measurement based on binocular vision system is heavily dependent on the accurate calibration of two rigidly-fixed cameras. In most traditional calibration methods, stereo parameters are iteratively optimized through the forward imaging process (FIP). However, the results can only guarantee the minimal 2D pixel errors, but not the minimal 3D reconstruction errors. To address this problem, a simple method to calibrate a stereo rig based on the backward projection process (BPP) is proposed. The position of a spatial point can be determined separately from each camera by planar constraints provided by the planar pattern target. Then combined with pre-defined spatial points, intrinsic and extrinsic parameters of the stereo-rig can be optimized by minimizing the total 3D errors of both left and right cameras. An extensive performance study for the method in the presence of image noise and lens distortions is implemented. Experiments conducted on synthetic and real data demonstrate the accuracy and robustness of the proposed method.

What Really Rigs Up RIG-I?

PubMed

Barik, Sailen

2016-01-01

RIG-I (retinoic acid-inducible gene 1) is an archetypal member of the cytoplasmic DEAD-box dsRNA helicase family (RIG-I-like receptors or RLRs), the members of which play essential roles in the innate immune response of the metazoan cell. RIG-I functions as a pattern recognition receptor that detects nonself RNA as a pathogen-associated molecular pattern (PAMP). However, the exact molecular nature of the viral RNAs that act as a RIG-I ligand has remained a mystery and a matter of debate. In this article, we offer a critical review of the actual viral RNAs that act as PAMPs to activate RIG-I, as seen from the perspective of a virologist, including a recent report that the viral Leader-read-through transcript is a novel and effective RIG-I ligand. © 2016 S. Karger AG, Basel.

Ubiquitin-like modifier FAT10 attenuates RIG-I mediated antiviral signaling by segregating activated RIG-I from its signaling platform

PubMed Central

Nguyen, Nhung T.H.; Now, Hesung; Kim, Woo-Jong; Kim, Nari; Yoo, Joo-Yeon

2016-01-01

RIG-I is a key cytosolic RNA sensor that mediates innate immune defense against RNA virus. Aberrant RIG-I activity leads to severe pathological states such as autosomal dominant multi-system disorder, inflammatory myophathies and dermatomyositis. Therefore, identification of regulators that ensure efficient defense without harmful immune-pathology is particularly critical to deal with RIG-I-associated diseases. Here, we presented the inflammatory inducible FAT10 as a novel negative regulator of RIG-I-mediated inflammatory response. In various cell lines, FAT10 protein is undetectable unless it is induced by pro-inflammatory cytokines. FAT10 non-covalently associated with the 2CARD domain of RIG-I, and inhibited viral RNA-induced IRF3 and NF-kB activation through modulating the RIG-I protein solubility. We further demonstrated that FAT10 was recruited to RIG-I-TRIM25 to form an inhibitory complex where FAT10 was stabilized by E3 ligase TRIM25. As the result, FAT10 inhibited the antiviral stress granules formation contains RIG-I and sequestered the active RIG-I away from the mitochondria. Our study presented a novel mechanism to dampen RIG-I activity. Highly accumulated FAT10 is observed in various cancers with pro-inflammatory environment, therefore, our finding which uncovered the suppressive effect of the accumulated FAT10 during virus-mediated inflammatory response may also provide molecular clue to understand the carcinogenesis related with infection and inflammation. PMID:26996158

Paramyxovirus V Proteins Interact with the RIG-I/TRIM25 Regulatory Complex and Inhibit RIG-I Signaling.

PubMed

Sánchez-Aparicio, Maria T; Feinman, Leighland J; García-Sastre, Adolfo; Shaw, Megan L

2018-03-15

Paramyxovirus V proteins are known antagonists of the RIG-I-like receptor (RLR)-mediated interferon induction pathway, interacting with and inhibiting the RLR MDA5. We report interactions between the Nipah virus V protein and both RIG-I regulatory protein TRIM25 and RIG-I. We also observed interactions between these host proteins and the V proteins of measles virus, Sendai virus, and parainfluenza virus. These interactions are mediated by the conserved C-terminal domain of the V protein, which binds to the tandem caspase activation and recruitment domains (CARDs) of RIG-I (the region of TRIM25 ubiquitination) and to the SPRY domain of TRIM25, which mediates TRIM25 interaction with the RIG-I CARDs. Furthermore, we show that V interaction with TRIM25 and RIG-I prevents TRIM25-mediated ubiquitination of RIG-I and disrupts downstream RIG-I signaling to the mitochondrial antiviral signaling protein. This is a novel mechanism for innate immune inhibition by paramyxovirus V proteins, distinct from other known V protein functions such as MDA5 and STAT1 antagonism. IMPORTANCE The host RIG-I signaling pathway is a key early obstacle to paramyxovirus infection, as it results in rapid induction of an antiviral response. This study shows that paramyxovirus V proteins interact with and inhibit the activation of RIG-I, thereby interrupting the antiviral signaling pathway and facilitating virus replication. Copyright © 2018 American Society for Microbiology.

Ubiquitin-like modifier FAT10 attenuates RIG-I mediated antiviral signaling by segregating activated RIG-I from its signaling platform.

PubMed

Nguyen, Nhung T H; Now, Hesung; Kim, Woo-Jong; Kim, Nari; Yoo, Joo-Yeon

2016-03-21

RIG-I is a key cytosolic RNA sensor that mediates innate immune defense against RNA virus. Aberrant RIG-I activity leads to severe pathological states such as autosomal dominant multi-system disorder, inflammatory myophathies and dermatomyositis. Therefore, identification of regulators that ensure efficient defense without harmful immune-pathology is particularly critical to deal with RIG-I-associated diseases. Here, we presented the inflammatory inducible FAT10 as a novel negative regulator of RIG-I-mediated inflammatory response. In various cell lines, FAT10 protein is undetectable unless it is induced by pro-inflammatory cytokines. FAT10 non-covalently associated with the 2CARD domain of RIG-I, and inhibited viral RNA-induced IRF3 and NF-kB activation through modulating the RIG-I protein solubility. We further demonstrated that FAT10 was recruited to RIG-I-TRIM25 to form an inhibitory complex where FAT10 was stabilized by E3 ligase TRIM25. As the result, FAT10 inhibited the antiviral stress granules formation contains RIG-I and sequestered the active RIG-I away from the mitochondria. Our study presented a novel mechanism to dampen RIG-I activity. Highly accumulated FAT10 is observed in various cancers with pro-inflammatory environment, therefore, our finding which uncovered the suppressive effect of the accumulated FAT10 during virus-mediated inflammatory response may also provide molecular clue to understand the carcinogenesis related with infection and inflammation.

Test Problem: Tilted Rayleigh-Taylor for 2-D Mixing Studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Andrews, Malcolm J.; Livescu, Daniel; Youngs, David L.

2012-08-14

The 'tilted-rig' test problem originates from a series of experiments (Smeeton & Youngs, 1987, Youngs, 1989) performed at AWE in the late 1980's, that followed from the 'rocket-rig' experiments (Burrows et al., 1984; Read & Youngs, 1983), and exploratory experiments performed at Imperial College (Andrews, 1986; Andrews and Spalding, 1990). A schematic of the experiment is shown in Figure 1, and comprises a tank filled with light fluid above heavy, and then 'tilted' on one side of the apparatus, thus causing an 'angled interface' to the acceleration history due to rockets. Details of the configuration given in the next chaptermore » include: fluids, dimensions, and other necessary details to simulate the experiment. Figure 2 shows results from two experiments, Case 110 (which is the source for this test problem) that has an Atwood number of 0.5, and Case 115 (a secondary source described in Appendix B), with Atwood of 0.9 Inspection of the photograph in Figure 2 (the main experimental diagnostic) for Case 110. reveals two main areas for mix development; 1) a large-scale overturning motion that produces a rising plume (spike) on the left, and falling plume (bubble) on the right, that are almost symmetric; and 2) a Rayleigh-Taylor driven mixing central mixing region that has a large-scale rotation associated with the rising and falling plumes, and also experiences lateral strain due to stretching of the interface by the plumes, and shear across the interface due to upper fluid moving downward and to the right, and lower fluid moving upward and to the left. Case 115 is similar but differs by a much larger Atwood of 0.9 that drives a strong asymmetry between a left side heavy spike penetration and a right side light bubble penetration. Case 110 is chosen as the source for the present test problem as the fluids have low surface tension (unlike Case 115) due the addition of a surfactant, the asymmetry small (no need to have fine grids for the spike), and there is extensive reasonable quality photographic data. The photographs in Figure 2 also reveal the appearance of a boundary layer at the left and right walls; this boundary layer has not been included in the test problem as preliminary calculations suggested it had a negligible effect on plume penetration and RT mixing. The significance of this test problem is that, unlike planar RT experiments such as the Rocket-Rig (Youngs, 1984), Linear Electric Motor - LEM (Dimonte, 1990), or the Water Tunnel (Andrews, 1992), the Tilted-Rig is a unique two-dimensional RT mixing experiment that has experimental data and now (in this TP) Direct Numerical Simulation data from Livescu and Wei. The availability of DNS data for the tilted-rig has made this TP viable as it provides detailed results for comparison purposes. The purpose of the test problem is to provide 3D simulation results, validated by comparison with experiment, which can be used for the development and validation of 2D RANS models. When such models are applied to 2D flows, various physics issues are raised such as double counting, combined buoyancy and shear, and 2-D strain, which have not yet been adequately addressed. The current objective of the test problem is to compare key results, which are needed for RANS model validation, obtained from high-Reynolds number DNS, high-resolution ILES or LES with explicit sub-grid-scale models. The experiment is incompressible and so is directly suitable for algorithms that are designed for incompressible flows (e.g. pressure correction algorithms with multi-grid); however, we have extended the TP so that compressible algorithms, run at low Mach number, may also be used if careful consideration is given to initial pressure fields. Thus, this TP serves as a useful tool for incompressible and compressible simulation codes, and mathematical models. In the remainder of this TP we provide a detailed specification; the next section provides the underlying assumptions for the TP, fluids, geometry details, boundary conditions (and alternative set-ups), initial conditions, and acceleration history (and ways to treat the acceleration ramp at the start of the experiment). This is followed by a section that defines data to be collected from the simulations, with results from the experiments and DNS from Livescu using the CFDNS code, and ILES simulations from Youngs using the compressible TURMOIL code and Andrews using the incompressible RTI3D code. We close the TP with concluding remarks, and Appendices that includes details of the sister Case 115, initial condition specifications for density and pressure fields. The Tilted-Rig Test Problem is intended to serve as a validation problem for RANS models, and as such we have provided ILES and DNS simulations in support of the test problem definition. The generally good agreement between experiment, ILES and DNS supports our assertion that the Tilted-Rig is useful, and the only 2-D TP that can be used to validate RANS models.« less

Design, fabrication, and performance of foil journal bearing for the brayton rotating unit

NASA Technical Reports Server (NTRS)

Licht, L.; Branger, M.

1973-01-01

Foil bearings were designed and manufactured to replace pivoted-shoe journal bearings in an existing Brayton Cycle turbo-alternator-compressor. The design of this unconventional rotor support was accomplished within the constraints and space limitations imposed by the present machine, and the substitution of foil bearings was effected without changes or modification other machine components. A housing and a test rig were constructed to incorporate the new foil-bearing support into a unified assemble with an air-driven rotor and the gimbal-mounted thrust bearing, seals, and shrouds of an actual Brayton Rotating Unit. The foil bearing required no external pressure source, and stable self-acting rotation was achieved at all speeds up to 43,200 rpm. Excellent wipe-wear characteristics of the foil bearing permitted well over 1000 start-stop cycles with no deterioriation of performance in the entire speed range.

Advanced Gas Turbine (AGT) powertrain system development for automotive applications

NASA Technical Reports Server (NTRS)

1984-01-01

Rotor dynamic instability investigations were conducted. Forward ball bearing hydraulic mount configurations were tested with little effect. Trial assembly of S/N 002 ceramic engine was initiated. Impeller design activities were completed on the straight line element (SLE) blade definition to address near-net-shape powder metal die forging. Performance characteristics of the Baseline Test 2A impeller were closely preserved. The modified blading design has been released for tooling procurement. Developmental testing of the diffusion flame combustor (DFC) for initial use in the S/N 002 2100 F ceramic structures engine was completed. A natural gas slave preheater was designed and fabricated. Preliminary regenerator static seal rig testing showed a significant reduction in leakage and sensitivity to stack height. Ceramic screening tests were completed and two complete sets of ceramic static structures were qualified for engine testing. Efforts on rotor dynamics development to resolve subsynchronous motion were continued.

Dynamic response of film thickness in spiral-groove face seals

NASA Technical Reports Server (NTRS)

Dirusso, E.

1985-01-01

Tests were performed on an inward- and an outward-pumping spiral-groove face seal to experimentally determine the film thickness response to seal seat motions and to gain insight into the effect of secondary seal friction on film thickness behavior. Film thickness, seal seat axial motion, seal frictional torque, and film axial load were recorded as functions of time. The experiments revealed that for sinusoidal axial oscillations of the seal seat, the primary ring followed the seal seat motion very well. For a skewed seal seat, however, the primary ring did not follow the seal seat motion, and load-carrying capacity was degraded. Secondary seal friction was varied over a wide range to determine its effect on film thickness dynamics. The seals were tested with ambient air at room temperature and atmospheric pressure as the fluid medium. The test speed ranged from 7000 to 20,000 rpm. Seal tangential velocity ranged from 34 to 98 m/sec (113 to 323 ft/sec).

Phosphorylation-mediated negative regulation of RIG-I antiviral activity.

PubMed

Gack, Michaela U; Nistal-Villán, Estanislao; Inn, Kyung-Soo; García-Sastre, Adolfo; Jung, Jae U

2010-04-01

Recognition of invading viruses by the host is elicited by cellular sensors which trigger signaling cascades that lead to type I interferon (IFN) gene expression. Retinoic acid-inducible gene I (RIG-I) has emerged as a key receptor for the detection of viral RNA in the cytosol, inducing IFN-mediated innate immune responses to limit viral replication through its interaction with MAVS (also called IPS-1, CARDIF, or VISA). Upon the recognition of viral RNA, the Lys-172 residue of RIG-I undergoes ubiquitination induced by tripartite motif protein 25 (TRIM25), an essential protein for antiviral signal transduction. Here we demonstrate that phosphorylation represents another regulatory mechanism for RIG-I-mediated antiviral activity. Using protein purification and mass spectrometry analysis, we identified three phosphorylation sites in the amino-terminal caspase recruitment domains (CARDs) of RIG-I. One of these residues, Thr-170, is located in close proximity to Lys-172, and we speculated that its phosphorylation may affect Lys-172 ubiquitination and functional activation of RIG-I. Indeed, a RIG-I mutant carrying a phosphomimetic Glu residue in place of Thr-170 loses TRIM25 binding, Lys-172 ubiquitination, MAVS binding, and downstream signaling ability. This suggests that phosphorylation of RIG-I at Thr-170 inhibits RIG-I-mediated antiviral signal transduction. Immunoblot analysis with a phospho-specific antibody showed that the phosphorylation of the RIG-I Thr-170 residue is present under normal conditions but rapidly declines upon viral infection. Our results indicate that Thr-170 phosphorylation and TRIM25-mediated Lys-172 ubiquitination of RIG-I functionally antagonize each other. While Thr-170 phosphorylation keeps RIG-I latent, Lys-172 ubiquitination enables RIG-I to form a stable complex with MAVS, thereby inducing IFN signal transduction.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Huttrer, G.W.

This report summarizes the investigation and evaluation of several {open_quotes}compact{close_quotes} drill rigs which could be used for drilling geothermal production wells. Use of these smaller rigs would save money by reducing mobilization costs, fuel consumption, crew sizes, and environmental impact. Advantages and disadvantages of currently-manufactured rigs are identified, and desirable characteristics for the {open_quotes}ideal{close_quotes} compact rig are defined. The report includes a detailed cost estimate of a specific rig, and an evaluation of the cost/benefit ratio of using this rig. Industry contacts for further information are given.

An Experimental Study of Cavitation Detection in a Centrifugal Pump Using Envelope Analysis

NASA Astrophysics Data System (ADS)

Tan, Chek Zin; Leong, M. Salman

Cavitation represents one of the most common faults in pumps and could potentially lead to a series of failure in mechanical seal, impeller, bearing, shaft, motor, etc. In this work, an experimental rig was setup to investigate cavitation detection using vibration envelope analysis method, and measured parameters included sound, pressure and flow rate for feasibility of cavitation detection. The experiment testing included 3 operating points of the centrifugal pump (B.E.P, 90% of B.E.P and 80% of B.E.P). Suction pressure of the centrifugal pump was decreased gradually until the inception point of cavitation. Vibration measurements were undertaken at various locations including casing, bearing, suction and discharge flange of the centrifugal pump. Comparisons of envelope spectrums under cavitating and non-cavitating conditions were presented. Envelope analysis was proven useful in detecting cavitation over the 3 testing conditions. During the normal operating condition, vibration peak synchronous to rotational speed was more pronounced. It was however during cavitation condition, the half order sub-harmonic vibration component was clearly evident in the envelope spectrums undertaken at all measurement locations except at the pump bearing. The possible explanation of the strong sub-harmonic (½ of BPF) during cavitation existence in the centrifugal pump was due to insufficient time for the bubbles to collapse completely before the end of the single cycle.

Design criteria monograph on transmission seals

NASA Technical Reports Server (NTRS)

Hayden, T. S.; Keller, C. H., Jr.

1975-01-01

Guide is based on experience obtained in wide variety of applications using lip, circumferential, and face seals. Particular attention is given to capabilities and lubrication of various seal types. Special limitations as a result of storage requirements, quality control, installation, operation, and removal are discussed.

TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity.

PubMed

Gack, Michaela U; Shin, Young C; Joo, Chul-Hyun; Urano, Tomohiko; Liang, Chengyu; Sun, Lijun; Takeuchi, Osamu; Akira, Shizuo; Chen, Zhijian; Inoue, Satoshi; Jung, Jae U

2007-04-19

Retinoic-acid-inducible gene-I (RIG-I; also called DDX58) is a cytosolic viral RNA receptor that interacts with MAVS (also called VISA, IPS-1 or Cardif) to induce type I interferon-mediated host protective innate immunity against viral infection. Furthermore, members of the tripartite motif (TRIM) protein family, which contain a cluster of a RING-finger domain, a B box/coiled-coil domain and a SPRY domain, are involved in various cellular processes, including cell proliferation and antiviral activity. Here we report that the amino-terminal caspase recruitment domains (CARDs) of RIG-I undergo robust ubiquitination induced by TRIM25 in mammalian cells. The carboxy-terminal SPRY domain of TRIM25 interacts with the N-terminal CARDs of RIG-I; this interaction effectively delivers the Lys 63-linked ubiquitin moiety to the N-terminal CARDs of RIG-I, resulting in a marked increase in RIG-I downstream signalling activity. The Lys 172 residue of RIG-I is critical for efficient TRIM25-mediated ubiquitination and for MAVS binding, as well as the ability of RIG-I to induce antiviral signal transduction. Furthermore, gene targeting demonstrates that TRIM25 is essential not only for RIG-I ubiquitination but also for RIG-I-mediated interferon- production and antiviral activity in response to RNA virus infection. Thus, we demonstrate that TRIM25 E3 ubiquitin ligase induces the Lys 63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity.

Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses

PubMed Central

Liu, Wei; Li, Jing; Zheng, Weinan; Shang, Yingli; Zhao, Zhendong; Wang, Shanshan; Bi, Yuhai; Zhang, Shuang; Xu, Chongfeng; Duan, Ziyuan; Zhang, Lianfeng; Wang, Yue L; Jiang, Zhengfan; Liu, Wenjun; Sun, Lei

2017-01-01

RIG-I is a key cytosolic pattern recognition receptor that interacts with MAVS to induce type I interferons (IFNs) against RNA virus infection. In this study, we found that cyclophilin A (CypA), a peptidyl-prolyl cis/trans isomerase, functioned as a critical positive regulator of RIG-I-mediated antiviral immune responses. Deficiency of CypA impaired RIG-I-mediated type I IFN production and promoted viral replication in human cells and mice. Upon Sendai virus infection, CypA increased the interaction between RIG-I and its E3 ubiquitin ligase TRIM25, leading to enhanced TRIM25-mediated K63-linked ubiquitination of RIG-I that facilitated recruitment of RIG-I to MAVS. In addition, CypA and TRIM25 competitively interacted with MAVS, thereby inhibiting TRIM25-induced K48-linked ubiquitination of MAVS. Taken together, our findings reveal an essential role of CypA in boosting RIG-I-mediated antiviral immune responses by controlling the ubiquitination of RIG-I and MAVS. DOI: http://dx.doi.org/10.7554/eLife.24425.001 PMID:28594325

Antiviral activity of human oligoadenylate synthetases-like (OASL) is mediated by enhancing retinoic acid-inducible gene I (RIG-I) signaling

PubMed Central

Zhu, Jianzhong; Zhang, Yugen; Ghosh, Arundhati; Cuevas, Rolando A.; Forero, Adriana; Dhar, Jayeeta; Ibsen, Mikkel Søes; Schmid-Burgk, Jonathan Leo; Schmidt, Tobias; Ganapathiraju, Madhavi K.; Fujita, Takashi; Hartmann, Rune; Barik, Sailen; Hornung, Veit; Coyne, Carolyn B.; Sarkar, Saumendra N.

2014-01-01

SUMMARY Virus infection is sensed in the cytoplasm by retinoic acid-inducible gene I (RIG-I, also known as DDX58), which requires RNA and polyubiquitin binding to induce type I interferon (IFN), and activate cellular innate immunity. We show that the human IFN-inducible oligoadenylate synthetases-like (OASL) protein had antiviral activity and mediated RIG-I activation by mimicking polyubiquitin. Loss of OASL expression reduced RIG-I signaling and enhanced virus replication in human cells. Conversely, OASL expression suppressed replication of a number of viruses in a RIG-I-dependent manner and enhanced RIG-I-mediated IFN induction. OASL interacted and colocalized with RIG-I, and through its C-terminal ubiquitin-like domain specifically enhanced RIG-I signaling. Bone marrow derived macrophages from mice deficient for Oasl2 showed that among the two mouse orthologs of human OASL; Oasl2 is functionally similar to human OASL. Our findings show a mechanism by which human OASL contributes to host antiviral responses by enhancing RIG-I activation. PMID:24931123

Structural features of influenza A virus panhandle RNA enabling the activation of RIG-I independently of 5′-triphosphate

PubMed Central

Lee, Mi-Kyung; Kim, Hee-Eun; Park, Eun-Byeol; Lee, Janghyun; Kim, Ki-Hun; Lim, Kyungeun; Yum, Seoyun; Lee, Young-Hoon; Kang, Suk-Jo; Lee, Joon-Hwa; Choi, Byong-Seok

2016-01-01

Retinoic acid-inducible gene I (RIG-I) recognizes specific molecular patterns of viral RNAs for inducing type I interferon. The C-terminal domain (CTD) of RIG-I binds to double-stranded RNA (dsRNA) with the 5′-triphosphate (5′-PPP), which induces a conformational change in RIG-I to an active form. It has been suggested that RIG-I detects infection of influenza A virus by recognizing the 5′-triphosphorylated panhandle structure of the viral RNA genome. Influenza panhandle RNA has a unique structure with a sharp helical bending. In spite of extensive studies of how viral RNAs activate RIG-I, whether the structural elements of the influenza panhandle RNA confer the ability to activate RIG-I signaling has been poorly explored. Here, we investigated the dynamics of the influenza panhandle RNA in complex with RIG-I CTD using NMR spectroscopy and showed that the bending structure of the panhandle RNA negates the requirement of a 5′-PPP moiety for RIG-I activation. PMID:27288441

Forming a Turbomachinery Seals Working Group - An Overview and Discussion

NASA Technical Reports Server (NTRS)

Proctor, Margaret P.

2007-01-01

A proposal to form a Turbomachinery Seals Working Group is discussed. Survey responses regarding the purpose, membership, and meeting frequency are presented as well as the areas of expertise and experience of the respondents. The types of seals used, designed, or sold, current work, and technical challenges of turbomachinery seals, their materials, analysis, geometry, manufacturing, maintenance, testing, and incorporation into engine systems are also presented.

Glass-to-Metal Seal Against Liquid Helium

NASA Technical Reports Server (NTRS)

Watkins, John L.; Gatewood, John R.

1987-01-01

Simple compression joint with indium gasket forms demountable seal for superfluids. Seal developed for metal lid on glass jar used in experiments on liquid helium. Glass container allows contents to be viewed for such purposes as calibration of liquid-level detectors and adjustments of displacement plungers. Seal contains liquid helium even when temperature drops below 2.19K. Made from inexpensive, commercially available materials and parts.

Performance of the coned-face end seal with regard to energy conservation

NASA Technical Reports Server (NTRS)

Sehnal, J.; Sedy, J.; Zobens, A.; Etsion, I.

1982-01-01

The effects of face coning on seal performance are evaluated with particular regard to the energy saving potential of convex conedface end seals as indicated by torque reduction. Experiments were conducted using a conventional carbon flat-face pusher-type seal, a coned-face pusher-type seal, coned-face bellows-type seal, and a modified coned-face pusher-type seal intended for dynamically unstable operation, with shaft rotation at up to 8000 rpm, pressures of up to 2758 kPa, and a petroleum-based turbine oil as lubricant. Torque at the seal interface is found to be reduced by 42% when the standard flat-face seal is replaced by a coned seal, although the leakage of the cone-face seal was 11 times greater. Reduction of seal balance from 76.1 to 51.3% resulted in an additional 44% reduction in torque, although at the expense of excessive leakage, but did not produce unstable operation. Face temperatures were reduced by 33-56 C and wear was also reduced greatly on the cone face seals. Seal performance is noted to be in agreement with analytical calculations.

Involvement of zebrafish RIG-I in NF-κB and IFN signaling pathways: insights into functional conservation of RIG-I in antiviral innate immunity.

PubMed

Nie, Li; Zhang, Ying-sheng; Dong, Wei-ren; Xiang, Li-xin; Shao, Jian-zhong

2015-01-01

The retinoic acid-inducible gene I (RIG-I) is a critical sensor for host recognition of RNA virus infection and initiation of antiviral signaling pathways in mammals. However, data on the occurrence and functions of this molecule in lower vertebrates are limited. In this study, we characterized an RIG-I homolog (DrRIG-I) from zebrafish. Structurally, this DrRIG-I shares a number of conserved functional domains/motifs with its mammalian counterparts, namely, caspase activation and recruitment domain, DExD/H box, a helicase domain, and a C-terminal domain. Functionally, stimulation with DrRIG-I CARD in zebrafish embryos significantly activated the NF-κB and IFN signaling pathways, leading to the expression of TNF-α, IL-8 and IFN-induced Mx, ISG15, and viperin. However, knockdown of TRIM25 (a pivotal activator for RIG-I receptors) significantly suppressed the induced activation of IFN signaling. Results suggested the functional conservation of RIG-I receptors in the NF-κB and IFN signaling pathways between teleosts and mammals, providing a perspective into the evolutionary history of RIG-I-mediated antiviral innate immunity. Copyright © 2014 Elsevier Ltd. All rights reserved.

Lapland longspur mortality at an oil well drilling rig site, Laramie County, Wyoming

USGS Publications Warehouse

Ramirez, Pedro; Dickerson, Kimberly K.; Lindstrom, Jim; Meteyer, Carol U.; Darrah, Scott

2015-01-01

Two hundred fifty-one Lapland longspur (Calcarius lapponicus) carcasses were recovered around an oil well drilling rig in Laramie County, Wyoming, USA, on December 13–14, 2010, apparent victims of a winter storm and “light entrapment” from the lights on the drilling rig during foggy conditions. We found Lapland longspur carcasses distributed around the drilling rig from 33 m to 171 m. Investigators did not find evidence of bird carcasses on the drilling rig deck or equipment immediately adjacent to the drilling rig. We ruled out chemical toxins and disease as a cause of mortality. Weather conditions, the circular depositional pattern of carcasses around the drilling rig, and bird necropsy results led investigators to conclude that the Lapland longspur mortality was the result of the migrating birds entering the area illuminated by the drilling rig lights in freezing fog and the birds repeatedly circling the drilling rig until they fell to the ground in exhaustion and dying from subsequent trauma. Further research is needed to understand how to most effectively adjust lighting of onshore drilling rigs to reduce the potential for avian light entrapment. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

Gene expression profile after activation of RIG-I in 5'ppp-dsRNA challenged DF1.

PubMed

Chen, Yang; Xu, Qi; Li, Yang; Liu, Ran; Huang, Zhengyang; Wang, Bin; Chen, Guohong

2016-12-01

Retinoic acid inducible gene I (RIG-I) can recognize influenza viruses and evoke the innate immune response. RIG-I is absent in the chicken genome, but is conserved in the genome of ducks. Lack of RIG-I renders chickens more susceptible to avian influenza infection, and the clinical symptoms are more prominent than in other poultry. It is unknown whether introduction of duck RIG-I into chicken cells can establish the immunity as is seen in ducks and the role of RIG-I in established immunity is unknown. In this study, a chicken cell strain with stable expression of duRIG-I was established by lentiviral infection, giving DF1/LV5-RIG-I, and a control strain DF1/LV5 was established in parallel. To verify stable, high level expression of duRIG-I in DF1 cells, the levels of duRIG-I mRNA and protein were determined by real-time RT-PCR and Western blot, respectively. Further, 5'triphosphate double stranded RNA (5'ppp-dsRNA) was used to mimic an RNA virus infection and the infected DF1/LV5-RIG-I and DF1/LV5 cells were subjected to high-throughput RNA-sequencing, which yielded 193.46 M reads and 39.07 G bases. A total of 278 differentially expressed genes (DEGs), i.e., duRIG-I-mediated responsive genes, were identified by RNA-seq. Among the 278 genes, 120 DEGs are annotated in the KEGG database, and the most reliable KEGG pathways are likely to be the signaling pathways of RIG-I like receptors. Functional analysis by Gene ontology (GO) indicates that the functions of these DEGs are primarily related to Type I interferon (IFN) signaling, IFN-β-mediated cellular responses and up-regulation of the RIG-I signaling pathway. Based on the shared genes among different pathways, a network representing crosstalk between RIG-I and other signaling pathways was constructed using Cytoscape software. The network suggests that RIG-mediated pathway may crosstalk with the Jak-STAT signaling pathway, Toll-like receptor signaling pathway, Wnt signaling pathway, ubiquitin-mediated proteolysis and MAPK signaling pathway during the transduction of antiviral signals. After screening, a group of key responsive genes in RIG-I-mediated signaling pathways, such as ISG12-2, Mx1, IFIT5, TRIM25, USP18, STAT1, STAT2, IRF1, IRF7 and IRF8, were tested for differential expression by real-time RT-PCR. In summary, by combining our results and the current literature, we propose a RIG-I-mediated signaling network in chickens. Copyright © 2016 Elsevier Ltd. All rights reserved.

Leakage Account for Radial Face Contact Seal in Aircraft Engine Support

NASA Astrophysics Data System (ADS)

Vinogradov, A. S.; Sergeeva, T. V.

2018-01-01

The article is dedicated to the development of a methodology for the radial face contact seal design taking into consideration the supporting elements deformations in different aircraft engine operating modes. Radial face contact seals are popular in the aircraft engines bearing support. However, there are no published leakage calculation methodologies of these seals. Radial face contact seal leakage is determined by the gap clearance in the carbon seal ring split. In turn, the size gap clearance depends on the deformation of the seal assembly parts and from the engine operation. The article shows the leakage detection sequence in the intershaft radial face contact seal of the compressor support for take-off and cruising modes. Evaluated calculated leakage values (2.4 g/s at takeoff and 0.75 g/s at cruising) go with experience in designing seals.

An Overview of the NASA FAP Hypersonics Project Airbreathing Propulsion Research

NASA Technical Reports Server (NTRS)

Auslender, A. H.; Suder, Kenneth L.; Thomas, Scott R.

2009-01-01

The propulsion research portfolio of the National Aeronautics and Space Administration Fundamental Aeronautics Program Hypersonics Project encompasses a significant number of technical tasks that are aligned to achieve mastery and intellectual stewardship of the core competencies in the hypersonic-flight regime. An overall coordinated programmatic and technical effort has been structured to advance the state-of-the-art, via both experimental and analytical efforts. A subset of the entire hypersonics propulsion research portfolio is presented in this overview paper. To this end, two programmatic research disciplines are discussed; namely, (1) the Propulsion Discipline, including three associated research elements: the X-51A partnership, the HIFiRE-2 partnership, and the Durable Combustor Rig, and (2) the Turbine-Based Combine Cycle Discipline, including three associated research elements: the Combined Cycle Engine Large Scale Inlet Mode Transition Experiment, the small-scale Inlet Mode Transition Experiment, and the High-Mach Fan Rig.

Experimental use of sawed and sealed joints to minimize thermal cracking : interim report - third year.

DOT National Transportation Integrated Search

2001-09-01

Saw and Seal is the process of introducing uniformly spaced saw joints to a bituminous : overlay in an attempt to eliminate or retard the formation of thermal and /or reflective : cracking. : Saw and Seal technology has been experimented with f...

Experimental use of sawed and sealed joints to minimize thermal cracking : final report - August 2004.

DOT National Transportation Integrated Search

2004-08-01

Saw and Seal is the process of introducing uniformly spaced saw joints to a bituminous overlay in an : attempt to eliminate or retard the formation of thermal and /or reflective cracking. : Saw and Seal technology has been experimented with for...

Mutual Regulation of NOD2 and RIG-I in Zebrafish Provides Insights into the Coordination between Innate Antibacterial and Antiviral Signaling Pathways.

PubMed

Nie, Li; Xu, Xiao-Xiao; Xiang, Li-Xin; Shao, Jian-Zhong; Chen, Jiong

2017-05-27

Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) and retinoic acid-inducible gene I (RIG-I) are two important cytosolic pattern recognition receptors (PRRs) in the recognition of pathogen-associated molecular patterns (PAMPs), initiating innate antibacterial and antiviral signaling pathways. However, the relationship between these PRRs, especially in teleost fish models, is rarely reported. In this article, we describe the mutual regulation of zebrafish NOD2 ( Dr NOD2) and RIG-I ( Dr RIG-I) in innate immune responses. Luciferase assays were conducted to determine the activation of NF-κB and interferon signaling. Morpholino-mediated knockdown and mRNA-mediated rescue were performed to further confirm the regulatory roles between Dr NOD2 and Dr RIG-I. Results showed that Dr NOD2 and Dr RIG-I shared conserved structural hallmarks with their mammalian counterparts, and activated Dr RIG-I signaling can induce Dr NOD2 production. Surprisingly, Dr NOD2-initiated signaling can also induce Dr RIG-I expression, indicating that a mutual regulatory mechanism may exist between them. Studies conducted using HEK293T cells and zebrafish embryos showed that Dr RIG-I could negatively regulate Dr NOD2-activated NF-κB signaling, and Dr NOD2 could inhibit Dr RIG-I-induced IFN signaling. Moreover, knocking down Dr RIG-I expression by morpholino could enhance Dr NOD2-initiated NF-κB activation, and vice versa, which could be rescued by their corresponding mRNAs. Results revealed a mutual feedback regulatory mechanism underlying NOD2 and RIG-I signaling pathways in teleosts. This mechanism reflects the coordination between cytosolic antibacterial and antiviral PRRs in the complex network of innate immunity.

RIG-I overexpression decreases mortality of cigarette smoke exposed mice during influenza A virus infection.

PubMed

Wang, Xiaoqiu; Wu, Wenxin; Zhang, Wei; Leland Booth, J; Duggan, Elizabeth S; Tian, Lili; More, Sunil; Zhao, Yan D; Sawh, Ravindranauth N; Liu, Lin; Zou, Ming-Hui; Metcalf, Jordan P

2017-09-02

Retinoic acid-inducible gene I (RIG-I) is an important regulator of virus-induced antiviral interferons (IFNs) and proinflammatory cytokines which participate in clearing viral infections. Cigarette smoke (CS) exposure increases the frequency and severity of respiratory tract infections. We generated a RIG-I transgenic (TG) mouse strain that expresses the RIG-I gene product under the control of the human lung specific surfactant protein C promoter. We compared the mortality and host immune responses of RIG-I TG mice and their litter-matched wild type (WT) mice following challenge with influenza A virus (IAV). RIG-I overexpression increased survival of IAV-infected mice. CS exposure increased mortality in WT mice infected with IAV. Remarkably, the effect of RIG-I overexpression on survival during IAV infection was enhanced in CS-exposed animals. CS-exposed IAV-infected WT mice had a suppressed innate response profile in the lung compared to sham-exposed IAV-infected WT mice in terms of the protein concentration, total cell count and inflammatory cell composition in the bronchoalveolar lavage fluid. RIG-I overexpression restored the innate immune response in CS-exposed mice to that seen in sham-exposed WT mice during IAV infection, and is likely responsible for enhanced survival in RIG-I TG mice as restoration preceded death of the animals. Our results demonstrate that RIG-I overexpression in mice is protective for CS enhanced susceptibility of smokers to influenza infection, and that CS mediated RIG-I suppression may be partially responsible for the increased morbidity and mortality of the mice exposed to IAV. Thus, optimizing the RIG-I response may be an important treatment strategy for CS-enhanced lung infections, particularly those due to IAV.

Performance Evaluation of a High Bandwidth Liquid Fuel Modulation Valve for Active Combustion Control

NASA Technical Reports Server (NTRS)

Saus, Joseph R.; DeLaat, John C.; Chang, Clarence T.; Vrnak, Daniel R.

2012-01-01

At the NASA Glenn Research Center, a characterization rig was designed and constructed for the purpose of evaluating high bandwidth liquid fuel modulation devices to determine their suitability for active combustion control research. Incorporated into the rig s design are features that approximate conditions similar to those that would be encountered by a candidate device if it were installed on an actual combustion research rig. The characterized dynamic performance measures obtained through testing in the rig are planned to be accurate indicators of expected performance in an actual combustion testing environment. To evaluate how well the characterization rig predicts fuel modulator dynamic performance, characterization rig data was compared with performance data for a fuel modulator candidate when the candidate was in operation during combustion testing. Specifically, the nominal and off-nominal performance data for a magnetostrictive-actuated proportional fuel modulation valve is described. Valve performance data were collected with the characterization rig configured to emulate two different combustion rig fuel feed systems. Fuel mass flows and pressures, fuel feed line lengths, and fuel injector orifice size was approximated in the characterization rig. Valve performance data were also collected with the valve modulating the fuel into the two combustor rigs. Comparison of the predicted and actual valve performance data show that when the valve is operated near its design condition the characterization rig can appropriately predict the installed performance of the valve. Improvements to the characterization rig and accompanying modeling activities are underway to more accurately predict performance, especially for the devices under development to modulate fuel into the much smaller fuel injectors anticipated in future lean-burning low-emissions aircraft engine combustors.

Sealing properties of mechanical seals for an axial flow blood pump.

PubMed

Tomioka, J; Mori, T; Yamazaki, K; Koyanagi, H

1999-08-01

A miniature intraventricular axial flow blood pump for left ventricular support is under development. One of the key technologies required for such pumps is sealing of the motor shaft. In this study, to prevent blood backflow into the motor side, mechanical seals were developed and their sealing properties investigated. In the experimental apparatus, the mechanical seal separated the bovine blood on the chamber side from the cooling water on the motor side. A leakage of the blood was measured by inductively coupled plasma (ICP) light emission analysis. The rate of hemolysis was measured by the cyanmethemoglobin method. Frictional torque acting on the shaft was measured by a torque transducer. In the experiments, the rotational speed of the shaft was changed from 1,000 to 10,000 rpm, and the contact force of the seal faces was changed from 1.96 to 4.31 N. To estimate lubrication regimes, the Stribeck curve, a diagram of the coefficient of friction against the bearing characteristic G number, was drawn. The results of the experiments showed that both the leakage of blood and the rate of hemolysis were very small. The friction loss was also very small. The mechanical seal was operated in various lubrication regimes, from a fluid lubrication regime to a mixed lubrication regime.

Innovative workover/drilling rigs to utilize hydraulics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Noran, D.

1975-09-29

While Western Gear Corp., Everett, Wash., is currently building 2 models of a hydraulic workover/drilling rig (one offshore and the other a trailer-mounted land rig), Bender Co., Bakersfield, Calif., already has an all-hydraulic servicing/drilling rig undergoing tests. The rigs are similar in that they eliminate the traveling block, crown block, draw works, chains, and sprockets found on the conventional rig. The major design innovation on the Western Gear model is the 260,000-lb-capacity hoisting system in which 2 hydraulic rams are anchored to the rig floor and carry all the pipe weight, thus eliminating the danger of the derrick's being pulledmore » in. Other changes involve the tripping system, a power swivel/elevator, and the control valves. Maintenance and labor cost reductions are expected to be substantial. The Bender Co. rig has a single-section mast that is a lever-lift-type derrick which serves as a guide for the rams and a support for the pipe-racking platform. Hoisting capacity depends on the number and size of the lifting rods (which support the crown sheaves) and the hydraulic pressure applied. Manufacturing and operating costs are expected to be less than for conventional rigs.« less

[Innate immune responses against viral infection and its suppression by viral proteins].

PubMed

Oshiumi, Hiroyuki; Matsumoto, Misako; Seya, Tsukasa

2013-01-01

Retinoic acid-inducible gene-I(RIG-I) is a cytoplasmic RNA helicase and a viral RNA sensor. RIG-I recognizes 5' triphosphate double-stranded RNA (dsRNA) and activates the IPS-1 adaptor molecule. The association of IPS-1 with RIG-I causes the formation of the prion-like structure of IPS-1. This structure is essential for activation of the signaling required for the induction of type I interferon (IFN), which possesses strong antiviral activity. Recent studies have revealed the novel factors involved in the RIG-I-dependent pathway. DDX3 and DDX60 RNA helicases associate with RIG-I and promote its binding to viral RNA. Riplet and TRIM25 ubiquitin ligase deliver Lys63-linked polyubiquitin moiety to RIG-I and result in signal activation. Several pathogenic viruses have evolved excellent systems to suppress type I IFN production. For example, NS3-4A of hepatitis C virus (HCV) cleaves IPS-1, which is the adaptor molecule of RIG-I, while the HCV core protein abrogates DDX3 function to suppress RIG-I-dependent IPS-1 activation, and the NS-1 of flu inhibits TRIM25 function to suppress RIG-I activation.

Improved Steam Turbine Leakage Control with a Brush Seal Design

NASA Astrophysics Data System (ADS)

Turnquist, Norman; Chupp, Raymond E.; Pastrana, Ryan; Wolfe, Chris; Burnett, Mark

2002-10-01

This paper presents an improved steam turbine leakage control system with a brush seal design. The contents include: 1) Typical Design Characteristics; 2) Typical Brush Seal Locations; 3) Reduced Leakage Rates; 4) Performance Benefits; 5) System Considerations; 6) Rotor Dynamics; 7) Laboratory Tests and 8) Field Experience.

Experimental use of sawed and sealed joints to minimize thermal cracking : interim report - second year.

DOT National Transportation Integrated Search

1999-08-01

"Saw and Seal" is the process of introducing uniformly spaced saw joints to a bituminous : overlay in an attempt to eliminate or retard the formation of thermal and /or reflective : cracking. : Saw and Seal technology has been experimented with for m...

Comparisons of Rig and Engine Dynamic Events in the Compressor of an Axi-Centrifugal Turboshaft Engine

NASA Technical Reports Server (NTRS)

Owen, A. Karl; Mattern, Duane L.; Le, Dzu K.

1996-01-01

Steady state and dynamic data were acquired in a T55-L-712 compressor rig. In addition, a T55-L-12 engine was instrumented and similar data were acquired. Rig and engine stall/surge data were analyzed using modal techniques. This paper compares rig and engine preliminary results for the ground idle (approximately 60% of design speed) point. The results of these analyses indicate both rig and engine dynamic event are preceded by indications of traveling wave energy in front of the compressor face. For both rig and engine, the traveling wave energy contains broad band energy with some prominent narrow peaks and, while the events are similar in many ways, some noticeable differences exist between the results of the analyses of rig data and engine data.

Experimental and numerical determination of the static critical pressure in ferrofluid seals

NASA Astrophysics Data System (ADS)

Horak, W.; Szczęch, M.

2013-02-01

Ferrofluids have various engineering applications; one of them are magnetic fluid seals for rotating shafts. There are various constructions of this type of seals, but the main difference is the number of sealing stages. The development of this construction is a complex process which requires knowledge of ferrofluid physical and rheological properties and the magnetic field distribution inside the sealing gap. One of the most important parameters of ferrofluid seals is the critical (burst) pressure. It is the pressure value at which a leak will occur. This study presents results of numerical simulation of magnetic field distribution inside the seal gap and calculations of the critical pressure value. The obtained pressure values were verified by experiments.

Integrating Oil Debris and Vibration Gear Damage Detection Technologies Using Fuzzy Logic

NASA Technical Reports Server (NTRS)

Dempsey, Paula J.; Afjeh, Abdollah A.

2002-01-01

A diagnostic tool for detecting damage to spur gears was developed. Two different measurement technologies, wear debris analysis and vibration, were integrated into a health monitoring system for detecting surface fatigue pitting damage on gears. This integrated system showed improved detection and decision-making capabilities as compared to using individual measurement technologies. This diagnostic tool was developed and evaluated experimentally by collecting vibration and oil debris data from fatigue tests performed in the NASA Glenn Spur Gear Fatigue Test Rig. Experimental data were collected during experiments performed in this test rig with and without pitting. Results show combining the two measurement technologies improves the detection of pitting damage on spur gears.

Spiral Bevel Gear Damage Detection Using Decision Fusion Analysis

NASA Technical Reports Server (NTRS)

Dempsey, Paula J.; Handschuh, Robert F.; Afjeh, Abdollah A.

2002-01-01

A diagnostic tool for detecting damage to spiral bevel gears was developed. Two different monitoring technologies, oil debris analysis and vibration, were integrated using data fusion into a health monitoring system for detecting surface fatigue pitting damage on gears. This integrated system showed improved detection and decision-making capabilities as compared to using individual monitoring technologies. This diagnostic tool was evaluated by collecting vibration and oil debris data from fatigue tests performed in the NASA Glenn Spiral Bevel Gear Fatigue Rigs. Data was collected during experiments performed in this test rig when pitting damage occurred. Results show that combining the vibration and oil debris measurement technologies improves the detection of pitting damage on spiral bevel gears.

Material Science

NASA Image and Video Library

2003-01-22

One of the first materials science experiments on the International Space Station -- the Solidification Using a Baffle in Sealed Ampoules (SUBSA) -- will be conducted during Expedition Five inside the Microgravity Science Glovebox. The glovebox is the first dedicated facility delivered to the Station for microgravity physical science research, and this experiment will be the first one operated inside the glovebox. The glovebox's sealed work environment makes it an ideal place for the furnace that will be used to melt semiconductor crystals. Astronauts can change out samples and manipulate the experiment by inserting their hands into a pair of gloves that reach inside the sealed box. Dr. Aleksandar Ostrogorsky, a materials scientist from the Rensselaer Polytechnic Institute, Troy, N.Y., and the principal investigator for the SUBSA experiment, uses the gloves to examine an ampoule like the ones used for his experiment inside the glovebox's work area. The Microgravity Science Glovebox and the SUBSA experiment are managed by NASA's Marshall Space Flight Center in Huntsville, Ala.

Viral RNA-Unprimed Rig-I Restrains Stat3 Activation in the Modulation of Regulatory T Cell/Th17 Cell Balance.

PubMed

Yang, Hui; Guo, He-Zhou; Li, Xian-Yang; Lin, Jian; Zhang, Wu; Zhao, Jun-Mei; Zhang, Hong-Xin; Chen, Sai-Juan; Chen, Zhu; Zhu, Jiang

2017-07-01

Innate immunity activation by viral RNA-primed retinoid acid inducible gene-I (Rig-I) in CD4 + T cells antagonizes TGFβ signaling to suppress the differentiation of regulatory T cells (Tregs). However, how viral RNA-unliganded Rig-I (apo-Rig-I) modulates Treg generation remains unclear. In this article, we show that, in the absence of viral infection, Treg differentiation of Rig-I -/- CD4 + T cells was compromised, in the presence of increased generation of Th17 cells and overactivation of Stat3, a critical regulator tilting the Treg/Th17 cell balance. Mechanistically, apo-Rig-I physically associates with Stat3, thereby inhibiting Jak1's association with Stat3 while facilitating Shp2's association to inhibit p-Stat3 levels. Interestingly, inhibition of Stat3 ameliorates the Treg/Th17 imbalance and the colitis observed in Rig-I -/- mice. Collectively, these results uncover an independent functional contribution of the apo-Rig-I/Stat3 interaction in the maintenance of Treg/Th17 cell balance. Copyright © 2017 by The American Association of Immunologists, Inc.

Negative regulation of RIG-I-mediated antiviral signaling by TRK-fused gene (TFG) protein.

PubMed

Lee, Na-Rae; Shin, Han-Bo; Kim, Hye-In; Choi, Myung-Soo; Inn, Kyung-Soo

2013-07-19

RIG-I (retinoic acid inducible gene I)-mediated antiviral signaling serves as the first line of defense against viral infection. Upon detection of viral RNA, RIG-I undergoes TRIM25 (tripartite motif protein 25)-mediated K63-linked ubiquitination, leading to type I interferon (IFN) production. In this study, we demonstrate that TRK-fused gene (TFG) protein, previously identified as a TRIM25-interacting protein, binds TRIM25 upon virus infection and negatively regulates RIG-I-mediated type-I IFN signaling. RIG-I-mediated IFN production and nuclear factor (NF)-κB signaling pathways were upregulated by the suppression of TFG expression. Furthermore, vesicular stomatitis virus (VSV) replication was significantly inhibited by small inhibitory hairpin RNA (shRNA)-mediated knockdown of TFG, supporting the suppressive role of TFG in RIG-I-mediated antiviral signaling. Interestingly, suppression of TFG expression increased not only RIG-I-mediated signaling but also MAVS (mitochondrial antiviral signaling protein)-induced signaling, suggesting that TFG plays a pivotal role in negative regulation of RNA-sensing, RIG-I-like receptor (RLR) family signaling pathways. Copyright © 2013 Elsevier Inc. All rights reserved.

Injury Rates on New and Old Technology Oil and Gas Rigs Operated by the Largest United States Onshore Drilling Contractor

PubMed Central

Blackley, David J.; Retzer, Kyla D.; Hubler, Warren G.; Hill, Ryan D.; Laney, A. Scott

2015-01-01

Background Occupational fatality rates among oil and gas extraction industry and specifically among drilling contractor workers are high compared to the U.S. all-industry average. There is scant literature focused on non-fatal injuries among drilling contractors, some of which have introduced engineering controls to improve rig efficiency and reduce injury risk. Methods We compared injury rates on new and old technology rigs operated by the largest U.S. drilling contractor during 2003–2012, stratifying by job type and grouping outcomes by injury severity and body part affected. Results Six hundred seventy-one injuries were recorded over 77.4 million person-hours. The rate on new rigs was 66% of that on old rigs. Roughnecks had lower injury rates on new rigs, largely through reduced limb injury rates. New rigs had lower rates in each non-fatal injury severity category. Conclusions For this company, new technology rigs appear to provide a safer environment for roughnecks. Future studies could include data from additional companies. PMID:25164118

Injury rates on new and old technology oil and gas rigs operated by the largest United States onshore drilling contractor.

PubMed

Blackley, David J; Retzer, Kyla D; Hubler, Warren G; Hill, Ryan D; Laney, A Scott

2014-10-01

Occupational fatality rates among oil and gas extraction industry and specifically among drilling contractor workers are high compared to the U.S. all-industry average. There is scant literature focused on non-fatal injuries among drilling contractors, some of which have introduced engineering controls to improve rig efficiency and reduce injury risk. We compared injury rates on new and old technology rigs operated by the largest U.S. drilling contractor during 2003-2012, stratifying by job type and grouping outcomes by injury severity and body part affected. Six hundred seventy-one injuries were recorded over 77.4 million person-hours. The rate on new rigs was 66% of that on old rigs. Roughnecks had lower injury rates on new rigs, largely through reduced limb injury rates. New rigs had lower rates in each non-fatal injury severity category. For this company, new technology rigs appear to provide a safer environment for roughnecks. Future studies could include data from additional companies. © 2014 Wiley Periodicals, Inc.

Turbulence and mixing from optimal perturbations to a stratified shear layer

NASA Astrophysics Data System (ADS)

Kaminski, Alexis; Caulfield, C. P.; Taylor, John

2014-11-01

The stability and mixing of stratified shear layers is a canonical problem in fluid dynamics with relevance to flows in the ocean and atmosphere. The Miles-Howard theorem states that a necessary condition for normal-mode instability in parallel, inviscid, steady stratified shear flows is that the gradient Richardson number, Rig is less than 1/4 somewhere in the flow. However, substantial transient growth of non-normal modes may be possible at finite times even when Rig > 1 / 4 everywhere in the flow. We have calculated the ``optimal perturbations'' associated with maximum perturbation energy gain for a stably-stratified shear layer. These optimal perturbations are then used to initialize direct numerical simulations. For small but finite perturbation amplitudes, the optimal perturbations grow at the predicted linear rate initially, but then experience sufficient transient growth to become nonlinear and susceptible to secondary instabilities, which then break down into turbulence. Remarkably, this occurs even in flows for which Rig > 1 / 4 everywhere. We will describe the nonlinear evolution of the optimal perturbations and characterize the resulting turbulence and mixing.

A Universal Rig for Supporting Large Hammer Drills: Reduced Injury Risk and Improved Productivity

PubMed Central

Rempel, David; Barr, Alan

2015-01-01

Drilling holes into concrete with heavy hammer and rock drills is one of the most physically demanding tasks performed in commercial construction and poses risks for musculoskeletal disorders, noise induced hearing loss, hand arm vibration syndrome and silicosis. The aim of this study was to (1) use a participatory process to develop a rig to support pneumatic rock drills or large electric hammer drills in order to reduce the health risks and (2) evaluate the usability of the rig. Seven prototype rigs for supporting large hammer drills were developed and modified with feedback from commercial contractors and construction workers. The final design was evaluated by laborers and electricians (N=29) who performed their usual concrete drilling with the usual method and the new rig. Subjective regional fatigue was significantly less in the neck, shoulders, hands and arms, and lower back) when using the universal rig compared to the usual manual method. Usability ratings for the rig were significantly better than the usual method on stability, control, drilling, accuracy, and vibration. Drilling time was reduced by approximately 50% with the rig. Commercial construction contractors, laborers and electricians who use large hammer drills for drilling many holes should consider using such a rig to prevent musculoskeletal disorders, fatigue, and silicosis. PMID:26005290

Rig-I regulates NF-κB activity through binding to Nf-κb1 3′-UTR mRNA

PubMed Central

Zhang, Hong-Xin; Liu, Zi-Xing; Sun, Yue-Ping; Lu, Shun-Yuan; Liu, Xue-Song; Huang, Qiu-Hua; Xie, Yin-Yin; Dang, Su-Ying; Zheng, Guang-Yong; Li, Yi-Xue; Kuang, Ying; Fei, Jian; Chen, Zhu; Wang, Zhu-Gang

2013-01-01

Retinoic acid inducible gene I (RIG-I) senses viral RNAs and triggers innate antiviral responses through induction of type I IFNs and inflammatory cytokines. However, whether RIG-I interacts with host cellular RNA remains undetermined. Here we report that Rig-I interacts with multiple cellular mRNAs, especially Nf-κb1. Rig-I is required for NF-κB activity via regulating Nf-κb1 expression at posttranscriptional levels. It interacts with the multiple binding sites within 3′-UTR of Nf-κb1 mRNA. Further analyses reveal that three distinct tandem motifs enriched in the 3′-UTR fragments can be recognized by Rig-I. The 3′-UTR binding with Rig-I plays a critical role in normal translation of Nf-κb1 by recruiting the ribosomal proteins [ribosomal protein L13 (Rpl13) and Rpl8] and rRNAs (18S and 28S). Down-regulation of Rig-I or Rpl13 significantly reduces Nf-κb1 and 3′-UTR–mediated luciferase expression levels. These findings indicate that Rig-I functions as a positive regulator for NF-κB signaling and is involved in multiple biological processes in addition to host antivirus immunity. PMID:23553835

Activation of duck RIG-I by TRIM25 is independent of anchored ubiquitin.

PubMed

Miranzo-Navarro, Domingo; Magor, Katharine E

2014-01-01

Retinoic acid inducible gene I (RIG-I) is a viral RNA sensor crucial in defense against several viruses including measles, influenza A and hepatitis C. RIG-I activates type-I interferon signalling through the adaptor for mitochondrial antiviral signaling (MAVS). The E3 ubiquitin ligase, tripartite motif containing protein 25 (TRIM25), activates human RIG-I through generation of anchored K63-linked polyubiquitin chains attached to lysine 172, or alternatively, through the generation of unanchored K63-linked polyubiquitin chains that interact non-covalently with RIG-I CARD domains. Previously, we identified RIG-I of ducks, of interest because ducks are the host and natural reservoir of influenza viruses, and showed it initiates innate immune signaling leading to production of interferon-beta (IFN-β). We noted that K172 is not conserved in RIG-I of ducks and other avian species, or mouse. Because K172 is important for both mechanisms of activation of human RIG-I, we investigated whether duck RIG-I was activated by TRIM25, and if other residues were the sites for attachment of ubiquitin. Here we show duck RIG-I CARD domains are ubiquitinated for activation, and ubiquitination depends on interaction with TRIM25, as a splice variant that cannot interact with TRIM25 is not ubiquitinated, and cannot be activated. We expressed GST-fusion proteins of duck CARD domains and characterized TRIM25 modifications of CARD domains by mass spectrometry. We identified two sites that are ubiquitinated in duck CARD domains, K167 and K193, and detected K63 linked polyubiquitin chains. Site directed mutagenesis of each site alone, does not alter the ubiquitination profile of the duck CARD domains. However, mutation of both sites resulted in loss of all attached ubiquitin and polyubiquitin chains. Remarkably, the double mutant duck RIG-I CARD still interacts with TRIM25, and can still be activated. Our results demonstrate that anchored ubiquitin chains are not necessary for TRIM25 activation of duck RIG-I.

Pigeon RIG-I Function in Innate Immunity against H9N2 IAV and IBDV.

PubMed

Xu, Wenping; Shao, Qiang; Zang, Yunlong; Guo, Qiang; Zhang, Yongchao; Li, Zandong

2015-07-22

Retinoic acid-inducible gene I (RIG-I), a cytosolic pattern recognition receptor (PRR), can sense various RNA viruses, including the avian influenza virus (AIV) and infectious bursal disease virus (IBDV), and trigger the innate immune response. Previous studies have shown that mammalian RIG-I (human and mice) and waterfowl RIG-I (ducks and geese) are essential for type I interferon (IFN) synthesis during AIV infection. Like ducks, pigeons are also susceptible to infection but are ineffective propagators and disseminators of AIVs, i.e., "dead end" hosts for AIVs and even highly pathogenic avian influenza (HPAI). Consequently, we sought to identify pigeon RIG-I and investigate its roles in the detection of A/Chicken/Shandong/ZB/2007 (H9N2) (ZB07), Gansu/Tianshui (IBDV TS) and Beijing/CJ/1980 (IBDV CJ-801) strains in chicken DF-1 fibroblasts or human 293T cells. Pigeon mRNA encoding the putative pigeon RIG-I analogs was identified. The exogenous expression of enhanced green fluorescence protein (EGFP)-tagged pigeon RIG-I and caspase activation and recruitment domains (CARDs), strongly induced antiviral gene (IFN-β, Mx, and PKR) mRNA synthesis, decreased viral gene (M gene and VP2) mRNA expression, and reduced the viral titers of ZB07 and IBDV TS/CJ-801 virus strains in chicken DF-1 cells, but not in 293T cells. We also compared the antiviral abilities of RIG-I proteins from waterfowl (duck and goose) and pigeon. Our data indicated that waterfowl RIG-I are more effective in the induction of antiviral genes and the repression of ZB07 and IBDV TS/CJ-801 strain replication than pigeon RIG-I. Furthermore, chicken melanoma differentiation associated gene 5(MDA5)/ mitochondrial antiviral signaling (MAVS) silencing combined with RIG-I transfection suggested that pigeon RIG-I can restore the antiviral response in MDA5-silenced DF-1 cells but not in MAVS-silenced DF-1 cells. In conclusion, these results demonstrated that pigeon RIG-I and CARDs have a strong antiviral ability against AIV H9N2 and IBDV in chicken DF-1 cells but not in human 293T cells.

SU-F-T-421: Dosimetry Change During Radiotherapy and Dosimetry Difference for Rigid and Deformed Registration in the Mid-Thoracic Esophageal Carcinoma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tao, C; Liu, T; Chen, J

Purpose: This study aimed to analyze dosimetry changes during radiotherapy for the mid-thoracic esophageal carcinoma, and investigate dosimetry difference between rigid and deformed registration. Methods: Twelve patients with primary middle thoracic esophageal carcinoma were selected randomly. Based on first CT scanning of each patient, plans-o were generated by experience physicists. After 20 fractions treatment, the corresponding plans-re were created with second CT scanning. And then, these two CT images were rigid and deformed registration respectively, and the dose was accumulated plan-o with plan-re. The dosimetry variation of these plans (plan-o: with 30 fractions, plan-rig: the accumulated dose with rigid registrationmore » and plan-def: the accumulated dose with deformed registration) were evaluated by paired T-test. Results: The V20 value of total lung were 32.68%, 30.3% and 29.71% for plan-o, plan-rig and plan-def respectively. The mean dose of total lung was 17.19 Gy, 16.67 Gy and 16.51 Gy for plan-o plan-rig and plan-def respectively. There were significant differences between plan-o and plan-rig or plan-def for both V20 and mean dose of total lung (with p= 0.003, p= 0.000 for V20 and p=0.008, p= 0.000 for mean dose respectively). There was no significant difference between plan-rig and plan-def (with p=0.118 for V20 and p=0.384 for mean dose). The max dose of spinal-cord was 41.95 Gy, 41.48 Gy and 41.4 Gy for plan-o, plan-rig and plan-def respectively. There were no significant differences for the max dose of spinal-cord between these plans. Conclusion: The target volume changes and anatomic position displacement of mid-thoracic esophageal carcinoma should not be neglected in clinics. These changes would cause overdose in normal tissue. Therefore, it is necessary to have another CT scanning and re-plan during the mid-thoracic esophageal carcinoma radiotherapy. And the dosimetry difference between rigid and deformed fusions was not found in this study.« less

The SSME HPFTP interstage seals: Analysis and experiments for leakage and reaction-force coefficients

NASA Technical Reports Server (NTRS)

Childs, D. W.

1983-01-01

An improved theory for the prediction of the rotordynamic coefficients of turbulent annular seals was developed. Predictions from the theory are compared to the experimental results and an approach for the direct calculation of empirical turbulent coefficients from test data are introduced. An improved short seal solution is shown to do a better job of calculating effective stiffness and damping coefficients than either the original short seal solution or a finite length solution. However, the original short seal solution does a much better job of predicting equivalent added mass coefficient.

Theory versus experiment for the rotordynamic coefficients of annular gas seals. I - Test facility and apparatus

NASA Technical Reports Server (NTRS)

Childs, D. W.; Nelson, C. E.; Nicks, C.; Scharrer, J.; Elrod, D.

1985-01-01

A facility and apparatus are described for determining the rotordynamic coefficients and leakage characteristics of annular gas seals. The coefficients and leakage characteristics of annular gas seals. The apparatus has a current top speed of 8000 cpm with a nominal seal diameter of 15.24 cmn (6 in.). The air supply unit yields a seal pressure ratio of approximately 7. An external shaker is used to excite the test rotor. The capability to independently calculate all rotordynamic coefficients at a given operating condition with one excitation frequency are discussed.

West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5.

PubMed

Zhang, Hong-Lei; Ye, Han-Qing; Liu, Si-Qing; Deng, Cheng-Lin; Li, Xiao-Dan; Shi, Pei-Yong; Zhang, Bo

2017-09-15

West Nile virus (WNV) is a mosquito-borne flavivirus that causes epidemics of encephalitis and viscerotropic disease worldwide. This virus has spread rapidly and has posed a significant public health threat since the outbreak in New York City in 1999. The interferon (IFN)-mediated antiviral response represents an important component of virus-host interactions and plays an essential role in regulating viral replication. Previous studies have suggested that multifunctional nonstructural proteins encoded by flaviviruses antagonize the host IFN response via various means in order to establish efficient viral replication. In this study, we demonstrated that the nonstructural protein 1 (NS1) of WNV antagonizes IFN-β production, most likely through suppression of retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) activation. In a dual-luciferase reporter assay, WNV NS1 significantly inhibited the activation of the IFN-β promoter after Sendai virus infection or poly(I·C) treatment. NS1 also suppressed the activation of the IFN-β promoter when it was stimulated by interferon regulatory factor 3 (IRF3)/5D or its upstream molecules in the RLR signaling pathway. Furthermore, NS1 blocked the phosphorylation and nuclear translocation of IRF3 upon stimulation by various inducers. Mechanistically, WNV NS1 targets RIG-I and melanoma differentiation-associated gene 5 (MDA5) by interacting with them and subsequently causing their degradation by the proteasome. Furthermore, WNV NS1 inhibits the K63-linked polyubiquitination of RIG-I, thereby inhibiting the activation of downstream sensors in the RLR signaling pathway. Taken together, our results reveal a novel mechanism by which WNV NS1 interferes with the host antiviral response. IMPORTANCE WNV Nile virus (WNV) has received increased attention since its introduction to the United States. However, the pathogenesis of this virus is poorly understood. This study demonstrated that the nonstructural protein 1 (NS1) of WNV antagonizes the induction of interferon beta (IFN-β) by interacting with and degrading retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5), which are crucial viral sensors in the host innate immune system. Further experiments suggested that NS1-mediated inhibition of the RIG-I-like receptor (RLR) signaling pathway involves inhibition of RIG-I K63-linked polyubiquitination and that the proteasome plays a role in RIG-I degradation. This study provides new insights into the regulation of WNV NS1 in the RLR signaling pathway and reveals a novel mechanism by which WNV evades the host innate immune response. The novel findings may guide us to discover new therapeutic targets and develop effective vaccines for WNV infections. Copyright © 2017 American Society for Microbiology.

Reaction of Basaltic Materials under High-Fidelity Venus Surface Conditions using the Glenn Extreme Environment Rig: First Results

NASA Technical Reports Server (NTRS)

Radoman-Shaw, Brandon; Harvey, Ralph; Costa, Gustavo; Nakley, Leah Michelle; Jacobson, Nathan S.

2016-01-01

Both historical and current investigations of Venus suggest that atmosphererock interactions play a critical role in the evolution of its atmosphere and crust. We have begun a series of systematic experiments designed to further our understanding of atmosphere-driven weathering and secondary mineralization of basaltic materials that may be occurring on Venus today. Our experiments expose representative igneous phases (mineral, glasses and rocks) to a high-fidelity simulation of Venus surface conditions using the NASA Glenn Extreme Environment Rig (GEER) located at the NASA Glenn Research Center in Cleveland, Ohio. GEER is a very large (800L) vessel capable of producing a long-term, high fidelity simulation of both the physical conditions (750 K and 92 bar) and atmospheric chemistry (down to the ppb-level) asso-ciated with the Venusian surface. As of this writing we have just finished the first of several planned experiments: a 42-day exposure of selected mineral, rocks and volcanic glasses. Our goal is to identify and prioritize the reactions taking place and better our understanding of their importance in Venus' climate history.

Hoisting and Rigging (Formerly Hoisting and Rigging Manual)

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1995-06-01

This standard is intended as a reference document to be used by supervisors, line managers, safety personnel, equipment operators, and any other personnel responsible for safety of hoisting and rigging operations at DOE sites. It quotes or paraphrases the US OSHA and ANSI requirements. It also encompasses, under one cover,hoisting and rigging requirements, codes, standards, and regulations, eliminating the need to maintain extensive (and often incomplete) libraries of hoisting and rigging standards throughout DOE. The standard occasionally goes beyond the minimum general industry standards established by OSHA and ANSI, and also delineates the more stringent requirements necessary to accomplish themore » complex, diversified, critical, and often hazardous hoisting and rigging work found with the DOE complex.« less

Investigation of Liner Characteristics in the NASA Langley Curved Duct Test Rig

NASA Technical Reports Server (NTRS)

Gerhold, Carl H.; Brown, Martha C.; Watson, Willie R.; Jones, Michael G.

2007-01-01

The Curved Duct Test Rig (CDTR), which is designed to investigate propagation of sound in a duct with flow, has been developed at NASA Langley Research Center. The duct incorporates an adaptive control system to generate a tone in the duct at a specific frequency with a target Sound Pressure Level and a target mode shape. The size of the duct, the ability to isolate higher order modes, and the ability to modify the duct configuration make this rig unique among experimental duct acoustics facilities. An experiment is described in which the facility performance is evaluated by measuring the sound attenuation by a sample duct liner. The liner sample comprises one wall of the liner test section. Sound in tones from 500 to 2400 Hz, with modes that are parallel to the liner surface of order 0 to 5, and that are normal to the liner surface of order 0 to 2, can be generated incident on the liner test section. Tests are performed in which sound is generated without axial flow in the duct and with flow at a Mach number of 0.275. The attenuation of the liner is determined by comparing the sound power in a hard wall section downstream of the liner test section to the sound power in a hard wall section upstream of the liner test section. These experimentally determined attenuations are compared to numerically determined attenuations calculated by means of a finite element analysis code. The code incorporates liner impedance values educed from measured data from the NASA Langley Grazing Incidence Tube, a test rig that is used for investigating liner performance with flow and with (0,0) mode incident grazing. The analytical and experimental results compare favorably, indicating the validity of the finite element method and demonstrating that finite element prediction tools can be used together with experiment to characterize the liner attenuation.

Vapor Deposition Rig

NASA Image and Video Library

2015-01-27

The Plasma Spray-Physical Vapor Deposition (PS-PVD) Rig at NASA Glenn Research Center. The rig helps develop coatings for next-generation aircraft turbine components and create more efficient engines.

Trophic and reproductive ecology of Red Snapper, Lutjanus campechanus, on natural and artificial reefs in the western Gulf of Mexico

NASA Astrophysics Data System (ADS)

Ajemian, M. J.; Wetz, J. J.; Brewton, R. A.; Downey, C. H.; Stunz, G. W.

2016-02-01

Energy exploration in the Gulf of Mexico (Gulf) has resulted in the addition of numerous oil and gas production rigs that have added structurally complex habitat to an area otherwise dominated by bare bottom. The impact of these artificial structures on fish populations is largely unknown and there is ongoing debate about their functionality. Red Snapper (Lutjanus campechanus), an ecologically and economically important sportfish to the region, use natural as well as the artificial reefs created by standing and reefed (toppled or cutoff) oil and gas rigs. However, little is known about how trophic and reproductive characteristics of Red Snapper vary over these multiple habitat types. We analyzed stable isotopic composition (δ13C, δ 15N) of epaxial muscle and compared reproductive potential of Red Snapper (155-855 mm TL) from standing rigs, reefed rigs, and natural hard-bottom habitats off Texas. Red Snapper from standing rig sites were isotopically enriched in δ 15N compared to lower relief habitats, suggesting they were feeding at a higher trophic level on standing rigs. While gonadosomatic indices (GSI) and comparative histology implied a similar spawning season among structure types, GSI was highest for both sexes at standing rigs. These initial results suggest that while standing rigs appear to provide more enriched food resources leading to higher Red Snapper reproductive capacity, the productivity of this species is similar between currently permitted rig decommissioning options (i.e., cutoff and toppled rigs) and natural hard-bottom habitats in the Gulf of Mexico.

Negative regulation of RIG-I-mediated antiviral signaling by TRK-fused gene (TFG) protein

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, Na-Rae; Shin, Han-Bo; Kim, Hye-In

2013-07-19

Highlights: •TRK-fused gene product (TFG) interacts with TRIM25 upon viral infection. •TFG negatively regulates RIG-I mediated antiviral signaling. •TFG depletion leads to enhanced viral replication. •TFG act downstream of MAVS. -- Abstract: RIG-I (retinoic acid inducible gene I)-mediated antiviral signaling serves as the first line of defense against viral infection. Upon detection of viral RNA, RIG-I undergoes TRIM25 (tripartite motif protein 25)-mediated K63-linked ubiquitination, leading to type I interferon (IFN) production. In this study, we demonstrate that TRK-fused gene (TFG) protein, previously identified as a TRIM25-interacting protein, binds TRIM25 upon virus infection and negatively regulates RIG-I-mediated type-I IFN signaling. RIG-I-mediatedmore » IFN production and nuclear factor (NF)-κB signaling pathways were upregulated by the suppression of TFG expression. Furthermore, vesicular stomatitis virus (VSV) replication was significantly inhibited by small inhibitory hairpin RNA (shRNA)-mediated knockdown of TFG, supporting the suppressive role of TFG in RIG-I-mediated antiviral signaling. Interestingly, suppression of TFG expression increased not only RIG-I-mediated signaling but also MAVS (mitochondrial antiviral signaling protein)-induced signaling, suggesting that TFG plays a pivotal role in negative regulation of RNA-sensing, RIG-I-like receptor (RLR) family signaling pathways.« less

Hepatitis B virus inhibits intrinsic RIG-I and RIG-G immune signaling via inducing miR146a

PubMed Central

Hou, Zhaohua; Zhang, Jian; Han, Qiuju; Su, Chenhe; Qu, Jing; Xu, Dongqing; Zhang, Cai; Tian, Zhigang

2016-01-01

Previous studies showed that hepatitis B virus (HBV), as a latency invader, attenuated host anti-viral immune responses. miRNAs were shown to be involved in HBV infection and HBV-related diseases, however, the precise role of miRNAs in HBV-mediated immunosuppression remains unclear. Here, we observed that down-regulated RIG-I like receptors might be one critical mechanism of HBV-induced suppression of type I IFN transcription in both HBV+ hepatoma cell lines and liver cancer tissues. Then, miR146a was demonstrated to negatively regulate the expression of RIG-I-like receptors by directly targeting both RIG-I and RIG-G. Further investigation showed that antagonizing miR146a by anti-sense inhibitors or sponge approach accelerated HBV clearance and reduced HBV load both in vitro and in a HBV-carrying mouse model. Therefore, our findings indicated that HBV-induced miR146a attenuates cell-intrinsic anti-viral innate immunity through targeting RIG-I and RIG-G, and silencing miR146a might be an effective target to reverse HBV-induced immune suppression. PMID:27210312

RIG-I antiviral signaling drives interleukin-23 production and psoriasis-like skin disease.

PubMed

Zhu, Huiyuan; Lou, Fangzhou; Yin, Qianqian; Gao, Yuanyuan; Sun, Yang; Bai, Jing; Xu, Zhenyao; Liu, Zhaoyuan; Cai, Wei; Ke, Fang; Zhang, Lingyun; Zhou, Hong; Wang, Hong; Wang, Gang; Chen, Xiang; Zhang, Hongxin; Wang, Zhugang; Ginhoux, Florent; Lu, Chuanjian; Su, Bing; Wang, Honglin

2017-05-01

Retinoic acid inducible-gene I (RIG-I) functions as one of the major sensors of RNA viruses. DDX58 , which encodes the RIG-I protein, has been newly identified as a susceptibility gene in psoriasis. Here, we show that the activation of RIG-I by 5'ppp-dsRNA, its synthetic ligand, directly causes the production of IL-23 and triggers psoriasis-like skin disease in mice. Repeated injections of IL-23 to the ears failed to induce IL-23 production and a full psoriasis-like skin phenotype, in either germ-free or RIG-I-deficient mice. RIG-I is also critical for a full development of skin inflammation in imiquimod (IMQ)-induced psoriasis-like mouse model. Furthermore, RIG-I-mediated endogenous IL-23 production was mainly confined to the CD11c + dendritic cells (DCs) via nuclear factor-kappa B (NF-κB) signaling, and stimulated RIG-I expression in an auto-regulatory feedback loop. Thus, our data suggest that the dysregulation in the antiviral immune responses of hosts through the innate pattern recognition receptors may trigger the skin inflammatory conditions in the pathophysiology of psoriasis. © 2017 The Authors. Published under the terms of the CC BY 4.0 license.

Subcellular Localizations of RIG-I, TRIM25, and MAVS Complexes

PubMed Central

Sánchez-Aparicio, M. T.; Ayllón, J.; Leo-Macias, A.; Wolff, T.

2016-01-01

ABSTRACT The retinoic acid-inducible gene 1 (RIG-I) signaling pathway is essential for the recognition of viruses and the initiation of host interferon (IFN)-mediated antiviral responses. Once activated, RIG-I interacts with polyubiquitin chains generated by TRIM25 and binds mitochondrial antiviral signaling protein (MAVS), leading to the production of type I IFN. We now show specific interactions among these key partners in the RLR pathway through the use of bimolecular fluorescence complementation (BiFC) and super-resolution microscopy. Dimers of RIG-I, TRIM25, and MAVS localize into different compartments. Upon activation, we show that TRIM25 is redistributed into cytoplasmic dots associated with stress granules, while RIG-I associates with TRIM25/stress granules and with mitochondrial MAVS. In addition, MAVS competes with TRIM25 for RIG-I binding, and this suggests that upon TRIM25-mediated activation of RIG-I, RIG-I moves away from TRIM25 to interact with MAVS at the mitochondria. For the first time, the distribution of these three proteins was analyzed at the same time in virus-infected cells. We also investigated how specific viral proteins modify some of the protein complexes in the pathway. The protease NS3/4A from hepatitis C virus redistributes the complexes RIG-I/MAVS and MAVS/MAVS but not RIG-I/TRIM25. In contrast, the influenza A virus NS1 protein interacts with RIG-I and TRIM25 in specific areas in the cell cytoplasm and inhibits the formation of TRIM25 homocomplexes but not the formation of RIG-I/TRIM25 heterocomplexes, preventing the formation of RIG-I/MAVS complexes. Thus, we have localized spatially in the cell different complexes formed between RIG-I, TRIM25, and MAVS, in the presence or absence of two viral IFN antagonistic proteins. IMPORTANCE The first line of defense against viral infections is the innate immune response. Viruses are recognized by pathogen recognition receptors, such as the RIG-I like receptor family, that activate a signaling cascade that induces IFN production. In the present study, we visualized, for the first time in cells, both in overexpression and endogenous levels, complexes formed among key proteins involved in this innate immune signaling pathway. Through different techniques we were able to analyze how these proteins are distributed and reorganized spatially within the cell in order to transmit the signal, leading to an efficient antiviral state. In addition, this work presents a new means by how, when, and where viral proteins can target these pathways and act against the host immune system in order to counteract the activation of the immune response. PMID:27807226

Subcellular Localizations of RIG-I, TRIM25, and MAVS Complexes.

PubMed

Sánchez-Aparicio, M T; Ayllón, J; Leo-Macias, A; Wolff, T; García-Sastre, A

2017-01-15

The retinoic acid-inducible gene 1 (RIG-I) signaling pathway is essential for the recognition of viruses and the initiation of host interferon (IFN)-mediated antiviral responses. Once activated, RIG-I interacts with polyubiquitin chains generated by TRIM25 and binds mitochondrial antiviral signaling protein (MAVS), leading to the production of type I IFN. We now show specific interactions among these key partners in the RLR pathway through the use of bimolecular fluorescence complementation (BiFC) and super-resolution microscopy. Dimers of RIG-I, TRIM25, and MAVS localize into different compartments. Upon activation, we show that TRIM25 is redistributed into cytoplasmic dots associated with stress granules, while RIG-I associates with TRIM25/stress granules and with mitochondrial MAVS. In addition, MAVS competes with TRIM25 for RIG-I binding, and this suggests that upon TRIM25-mediated activation of RIG-I, RIG-I moves away from TRIM25 to interact with MAVS at the mitochondria. For the first time, the distribution of these three proteins was analyzed at the same time in virus-infected cells. We also investigated how specific viral proteins modify some of the protein complexes in the pathway. The protease NS3/4A from hepatitis C virus redistributes the complexes RIG-I/MAVS and MAVS/MAVS but not RIG-I/TRIM25. In contrast, the influenza A virus NS1 protein interacts with RIG-I and TRIM25 in specific areas in the cell cytoplasm and inhibits the formation of TRIM25 homocomplexes but not the formation of RIG-I/TRIM25 heterocomplexes, preventing the formation of RIG-I/MAVS complexes. Thus, we have localized spatially in the cell different complexes formed between RIG-I, TRIM25, and MAVS, in the presence or absence of two viral IFN antagonistic proteins. The first line of defense against viral infections is the innate immune response. Viruses are recognized by pathogen recognition receptors, such as the RIG-I like receptor family, that activate a signaling cascade that induces IFN production. In the present study, we visualized, for the first time in cells, both in overexpression and endogenous levels, complexes formed among key proteins involved in this innate immune signaling pathway. Through different techniques we were able to analyze how these proteins are distributed and reorganized spatially within the cell in order to transmit the signal, leading to an efficient antiviral state. In addition, this work presents a new means by how, when, and where viral proteins can target these pathways and act against the host immune system in order to counteract the activation of the immune response. Copyright © 2017 American Society for Microbiology.

Development of braided rope engine seals

NASA Technical Reports Server (NTRS)

Ko, Frank K.; Cai, Zhong; Mutharasan, Rajakkannu; Steinetz, Bruce M.

1994-01-01

In this study, after reviewing current seal design concepts, the potential of textile structures for seal design is examined from the material, structural, and fabrication points of view. Braided structures are identified as potential candidates for hypersonic seal structures because of their conformability and design flexibility. A large family of braided structures using 2-D and 3-D architecture can be designed using well established methods to produce a wide range of braiding yarn orientation for wear resistance as well as seal porosity control. As a first demonstration of the approach, 2-D braided fiberglass seals were fabricated according to a factorial design experiment by varying braiding angles, fractional longitudinal fibers, and preload pressure levels. Factorial diagrams and response surfaces were constructed to elucidate the inter-relationship of the braiding parameters as well as the effect of preload pressures on leakage resistance of the seal. It was found that seal resistance is a strong function of fractional longitudinal fiber content. As braiding angle increases, seal leakage resistance increases, especially at high preload pressures and in seals having high proportion of longitudinal fibers.

Modeling and optimization aspects of radiation induced grafting of 4-vinylpyridene onto partially fluorinated films

NASA Astrophysics Data System (ADS)

Nasef, Mohamed Mahmoud; Ahmad Ali, Amgad; Saidi, Hamdani; Ahmad, Arshad

2014-01-01

Modeling and optimization aspects of radiation induced grafting (RIG) of 4-vinylpyridine (4-VP) onto partially fluorinated polymers such as poly(ethylene-co-tetrafluoroethene) (ETFE) and poly(vinylidene fluoride) (PVDF) films were comparatively investigated using response surface method (RSM). The effects of independent parameters: absorbed dose, monomer concentration, grafting time and reaction temperature on the response, grafting yield (GY) were correlated through two quadratic models. The results of this work confirm that RSM is a reliable tool not only for optimization of the reaction parameters and prediction of GY in RIG processes, but also for the reduction of the number of the experiments, monomer consumption and absorbed dose leading to an improvement of the overall reaction cost.

46 CFR 162.050-17 - Separator test rig.

Code of Federal Regulations, 2014 CFR

2014-10-01

... diagram of a typical test rig is shown in Figure 162.050-17(a). FIGURE 162.050-17(a)—SEPARATOR TEST RIG... discharge side. (c) The inlet piping of the test rig must be sized so that— (1) Influent water flows at a Reynolds Number of at least 10,000; (2) The influent flow rate is between one and three meters per second...

46 CFR 162.050-17 - Separator test rig.

Code of Federal Regulations, 2011 CFR

2011-10-01

... diagram of a typical test rig is shown in Figure 162.050-17(a). FIGURE 162.050-17(a)—SEPARATOR TEST RIG... discharge side. (c) The inlet piping of the test rig must be sized so that— (1) Influent water flows at a Reynolds Number of at least 10,000; (2) The influent flow rate is between one and three meters per second...

46 CFR 162.050-17 - Separator test rig.

Code of Federal Regulations, 2010 CFR

2010-10-01

... diagram of a typical test rig is shown in Figure 162.050-17(a). FIGURE 162.050-17(a)—SEPARATOR TEST RIG... discharge side. (c) The inlet piping of the test rig must be sized so that— (1) Influent water flows at a Reynolds Number of at least 10,000; (2) The influent flow rate is between one and three meters per second...

46 CFR 162.050-17 - Separator test rig.

Code of Federal Regulations, 2013 CFR

2013-10-01

... diagram of a typical test rig is shown in Figure 162.050-17(a). FIGURE 162.050-17(a)—SEPARATOR TEST RIG... discharge side. (c) The inlet piping of the test rig must be sized so that— (1) Influent water flows at a Reynolds Number of at least 10,000; (2) The influent flow rate is between one and three meters per second...

46 CFR 162.050-17 - Separator test rig.

Code of Federal Regulations, 2012 CFR

2012-10-01

... diagram of a typical test rig is shown in Figure 162.050-17(a). FIGURE 162.050-17(a)—SEPARATOR TEST RIG... discharge side. (c) The inlet piping of the test rig must be sized so that— (1) Influent water flows at a Reynolds Number of at least 10,000; (2) The influent flow rate is between one and three meters per second...

Robust Lys63-Linked Ubiquitination of RIG-I Promotes Cytokine Eruption in Early Influenza B Virus Infection

PubMed Central

Jiang, Jingwen; Fan, Wenhui; Zheng, Weinan; Yu, Meng; Chen, Can; Sun, Lei; Bi, Yuhai; Ding, Chan; Gao, George F.

2016-01-01

ABSTRACT Influenza A and B virus infections both cause a host innate immunity response. Here, we report that the robust production of type I and III interferons (IFNs), IFN-stimulated genes, and proinflammatory factors can be induced by influenza B virus rather than influenza A virus infection in alveolar epithelial (A549) cells during early infection. This response is mainly dependent on the retinoic acid-inducible gene I (RIG-I)-mediated signaling pathway. Infection by influenza B virus promotes intense Lys63-linked ubiquitination of RIG-I, resulting in cytokine eruption. It is known that the influenza A virus NS1 protein (NS1-A) interacts with RIG-I and TRIM25 to suppress the activation of RIG-I-mediated signaling. However, the present results indicate that the influenza B virus NS1 protein (NS1-B) is unable to interact with RIG-I but engages in the formation of a RIG-I/TRIM25/NS1-B ternary complex. Furthermore, we demonstrate that the N-terminal RNA-binding domain (RBD) of NS1-B is responsible for interaction with TRIM25 and that this interaction blocks the inhibitory effect of the NS1-B C-terminal effector domain (TED) on RIG-I ubiquitination. Our findings reveal a novel mechanism for the host cytokine response to influenza B virus infection through regulatory interplay between host and viral proteins. IMPORTANCE Influenza B virus generally causes local mild epidemics but is occasionally lethal to individuals. Existing studies describe the broad characteristics of influenza B virus epidemiology and pathology. However, to develop better prevention and treatments for the disease, determining the concrete molecular mechanisms of pathogenesis becomes pivotal to understand how the host reacts to the challenge of influenza B virus. Thus, we aimed to characterize the host innate immune response to influenza B virus infection. Here, we show that vigorous Lys63-linked ubiquitination of RIG-I and cytokine eruption dependent on RIG-I-mediated signal transduction are induced by virus infection. Additionally, TRIM25 positively regulates RIG-I-mediated signaling by ablating the inhibitory function of NS1-B on RIG-I ubiquitination. PMID:27122586

Activation of Duck RIG-I by TRIM25 Is Independent of Anchored Ubiquitin

PubMed Central

Miranzo-Navarro, Domingo; Magor, Katharine E.

2014-01-01

Retinoic acid inducible gene I (RIG-I) is a viral RNA sensor crucial in defense against several viruses including measles, influenza A and hepatitis C. RIG-I activates type-I interferon signalling through the adaptor for mitochondrial antiviral signaling (MAVS). The E3 ubiquitin ligase, tripartite motif containing protein 25 (TRIM25), activates human RIG-I through generation of anchored K63-linked polyubiquitin chains attached to lysine 172, or alternatively, through the generation of unanchored K63-linked polyubiquitin chains that interact non-covalently with RIG-I CARD domains. Previously, we identified RIG-I of ducks, of interest because ducks are the host and natural reservoir of influenza viruses, and showed it initiates innate immune signaling leading to production of interferon-beta (IFN-β). We noted that K172 is not conserved in RIG-I of ducks and other avian species, or mouse. Because K172 is important for both mechanisms of activation of human RIG-I, we investigated whether duck RIG-I was activated by TRIM25, and if other residues were the sites for attachment of ubiquitin. Here we show duck RIG-I CARD domains are ubiquitinated for activation, and ubiquitination depends on interaction with TRIM25, as a splice variant that cannot interact with TRIM25 is not ubiquitinated, and cannot be activated. We expressed GST-fusion proteins of duck CARD domains and characterized TRIM25 modifications of CARD domains by mass spectrometry. We identified two sites that are ubiquitinated in duck CARD domains, K167 and K193, and detected K63 linked polyubiquitin chains. Site directed mutagenesis of each site alone, does not alter the ubiquitination profile of the duck CARD domains. However, mutation of both sites resulted in loss of all attached ubiquitin and polyubiquitin chains. Remarkably, the double mutant duck RIG-I CARD still interacts with TRIM25, and can still be activated. Our results demonstrate that anchored ubiquitin chains are not necessary for TRIM25 activation of duck RIG-I. PMID:24466302

Expression levels of the innate response gene RIG-I and its regulators RNF125 and TRIM25 in HIV-1-infected adult and pediatric individuals.

PubMed

Britto, Alan M A; Amoedo, Nívea D; Pezzuto, Paula; Afonso, Adriana O; Martínez, Ana M B; Silveira, Jussara; Sion, Fernando S; Machado, Elizabeth S; Soares, Marcelo A; Giannini, Ana L M

2013-07-31

TLRs (Toll-like receptors) and RLRs (RIG-I-like receptors) mediate innate immune responses by detecting microorganism invasion. RIG-I activation results in the production of interferon (IFN) type 1 and IFN responsive genes (ISGs). As the ubiquitin ligases RNF125 and TRIM25 are involved in regulating RIG-I function, our aim was to assess whether the levels of these three genes vary between healthy and HIV-infected individuals and whether these levels are related to disease progression. Gene expression analyses for RIG-I, RNF125, and TRIM25 were performed for HIV-infected adults and the children's peripheral blood mononuclear cells (PBMCs). Reverse transcription-quantitative PCRs (RT-qPCRs) were performed in order to quantify the expression levels of RIG-I, RNF125 and TRIM25 from PBMCs purified from control or HIV-infected individuals. Controls express higher levels of the three genes when compared to HIV-infected patients. These expressions are clearly distinct between healthy and progressors, and are reproduced in adults and children. In controls, RNF125 is the highest expressed gene, whereas in progressors, RIG-I is either the highest expressed gene or is expressed similarly to RNF125 and TRIM25. A pattern of expression of RIG-I, RNF125, and TRIM25 genes in HIV patients is evident. The high expression of RNF125 in healthy individuals reflects the importance of keeping RIG-I function off, inhibiting unnecessary IFN production. Consistent with this assumption, RNF125 levels are lower in HIV patients and importantly, the RNF125/RIG-I ratio is lower in patients who progress to AIDS. Our results might help to predict disease progression and unveil the role of poorly characterized host genes during HIV infection.

An Experimental Study of the Structure of Turbulent Non-Premixed Jet Flames in Microgravity

NASA Astrophysics Data System (ADS)

Boxx, Isaac; Idicheria, Cherian; Clemens, Noel

2000-11-01

The aim of this work is to investigate the structure of transitional and turbulent non-premixed jet flames under microgravity conditions. The microgravity experiments are being conducted using a newly developed drop rig and the University of Texas 1.5 second drop tower. The rig itself measures 16”x33”x38” and contains a co-flowing round jet flame facility, flow control system, CCD camera, and data/image acquisition computer. These experiments are the first phase of a larger study being conducted at the NASA Glenn Research Center 2.2 second drop tower facility. The flames being studied include methane and propane round jet flames at jet exit Reynolds numbers as high as 10,000. The primary diagnostic technique employed is emission imaging of flame luminosity using a relatively high-speed (350 fps) CCD camera. The high-speed images are used to study flame height, flame tip dynamics and burnout characteristics. Results are compared to normal gravity experimental results obtained in the same apparatus.

Rigged or rigorous? Partnerships for research and evaluation of complex social problems: Lessons from the field of violence against women and girls.

PubMed

Zimmerman, Cathy; Michau, Lori; Hossain, Mazeda; Kiss, Ligia; Borland, Rosilyne; Watts, Charlotte

2016-09-01

There is growing demand for robust evidence to address complex social phenomena such as violence against women and girls (VAWG). Research partnerships between scientists and non-governmental or international organizations (NGO/IO) are increasingly popular, but can pose challenges, including concerns about potential conflicts of interest. Drawing on our experience collaborating on VAWG research, we describe challenges and contributions that NGO/IO and academic partners can make at different stages of the research process and the effects that collaborations can have on scientific inquiry. Partners may struggle with differing priorities and misunderstandings about roles, limitations, and intentions. Benefits of partnerships include a shared vision of study goals, differing and complementary expertise, mutual respect, and a history of constructive collaboration. Our experience suggests that when investigating multi-faceted social problems, instead of 'rigging' study results, research collaborations can strengthen scientific rigor and offer the greatest potential for impact in the communities we seek to serve.

RIG-I in RNA virus recognition

PubMed Central

Kell, Alison M.; Gale, Michael

2015-01-01

Antiviral immunity is initiated upon host recognition of viral products via non-self molecular patterns known as pathogen-associated molecular patterns (PAMPs). Such recognition initiates signaling cascades that induce intracellular innate immune defenses and an inflammatory response that facilitates development of the acquired immune response. The retinoic acid-inducible gene I (RIG-I) and the RIG-I-like receptor (RLR) protein family are key cytoplasmic pathogen recognition receptors that are implicated in the recognition of viruses across genera and virus families, including functioning as major sensors of RNA viruses, and promoting recognition of some DNA viruses. RIG-I, the charter member of the RLR family, is activated upon binding to PAMP RNA. Activated RIG-I signals by interacting with the adapter protein MAVS leading to a signaling cascade that activates the transcription factors IRF3 and NF-κB. These actions induce the expression of antiviral gene products and the production of type I and III interferons that lead to an antiviral state in the infected cell and surrounding tissue. RIG-I signaling is essential for the control of infection by many RNA viruses. Recently, RIG-I crosstalk with other pathogen recognition receptors and components of the inflammasome has been described. In this review, we discuss the current knowledge regarding the role of RIG-I in recognition of a variety of virus families and its role in programming the adaptive immune response through cross-talk with parallel arms of the innate immune system, including how RIG-I can be leveraged for antiviral therapy. PMID:25749629

Sealing And Filling Cracks In Asphalt Pavements

DOT National Transportation Integrated Search

1999-11-01

The primary objective of the crack treatment experiment was to determine the most effective and economical materials and methods for conducting crack-sealing and crack-filling operations. Secondary objectives included the identification of performanc...

An implantable centrifugal blood pump for long term circulatory support.

PubMed

Yamazaki, K; Litwak, P; Kormos, R L; Mori, T; Tagusari, O; Antaki, J F; Kameneva, M; Watach, M; Gordon, L; Umezu, M; Tomioka, J; Koyanagi, H; Griffith, B P

1997-01-01

A compact centrifugal blood pump was developed as an implantable left ventricular assist system. The impeller diameter is 40 mm and the pump dimensions are 55 x 64 mm. This first prototype was fabricated from titanium alloy, resulting in a pump weight of 400 g including a brushless DC motor. Weight of the second prototype pump was reduced to 280 g. The entire blood contacting surface is coated with diamond like carbon to improve blood compatibility. Flow rates of over 7 L/min against 100 mmHg pressure at 2,500 rpm with 9 W total power consumption have been measured. A newly designed mechanical seal with a recirculating purge system ("Cool-Seal") is used as a shaft seal. In this seal system, seal temperature is kept under 40 degrees C to prevent heat denaturation of blood proteins. Purge fluid also cools the pump motor coil and journal bearing. The purge fluid is continuously purified and sterilized by an ultrafiltration filter incorporated into the paracorporeal drive console. In vitro experiments with bovine blood demonstrated an acceptably low hemolysis rate (normalized index of hemolysis = 0.005 +/- 0.002 g/100 L). In vivo experiments are currently ongoing using calves. Via left thoracotomy, left ventricular apex-descending aorta bypass was performed utilizing a PTFE (Polytetrafluoroethylene) vascular graft, with the pump placed in the left thoracic cavity. In two in vivo experiments, pump flow rate was maintained at 5-8 L/min, and pump power consumption remained stable at 9-10 W. All plasma free hemoglobin levels were measured at < 15 mg/dl. The seal system has demonstrated good seal capability with negligible purge fluid consumption (< 0.5 ml/ day). Both animals remain under observation after 162 and 91 days of continuous pump function.

Analysis and design of trial well mooring in deepwater of the South China Sea

NASA Astrophysics Data System (ADS)

Guo, Yongfeng; Ji, Shaojun; Tang, Changquan; Li, Jiansong; Zhong, Huiquan; Ian, Ong Chin Yam

2012-06-01

Mooring systems play an important role for semi-submersible rigs that drill in deepwater. A detailed analysis was carried out on the mooring of a semi-submersible rig that conducted a trial well drilling at a deepwater location in the South China Sea in 2009. The rig was 30 years old and had a shallow platform with a designed maximum operating water depth of 457 m. Following the mooring analysis, a mooring design was given that requires upgrading of the rig's original mooring system. The upgrade included several innovations, such as installing eight larger anchors, i.e. replacing the original anchors and inserting an additional 600 m of steel wires with the existing chains. All this was done to enhance the mooring capability of the rig in order for the rig to be held in position to conduct drilling at a water depth of 476 m. The overall duration of the drilling was 50 days and the upgraded mooring system proved to be efficient in achieving the goal of keeping the rig stationary while it was drilling the trial well in the South China Sea. This successful campaign demonstrates that an older semi-submersible rig can take on drilling in deep water after careful design and proper upgrading and modification to the original mooring system.

Advanced Gas Turbine (AGT) technology development

NASA Technical Reports Server (NTRS)

1983-01-01

A 74.5 kW (100 hp) automotive gas turbine was evaluated. The engine structure, bearings, oil system, and electronics were demonstrated and no shaft dynamics or other vibration problem were encountered. Areas identified during the five tests are the scroll retention features, and transient thermal deflection of turbine backplates. Modifications were designed. Seroll retention is addressed by modifying the seal arrangement in front of the gasifier turbine assembly, which will increase the pressure load on the scroll in the forward direction and thereby increase the retention forces. the backplate thermal deflection is addressed by geometric changes and thermal insulation to reduce heat input. Combustor rig proof testing of two ceramic combustor assemblies was completed. The combustor was modified to incorporate slots and reduce sharp edges, which should reduce thermal stresses. The development work focused on techniques to sinter these barrier materials onto the ceramic rotors with successes for both material systems. Silicon carbide structural parts, including engine configuration gasifier rotors (ECRs), preliminary gasifier scroll parts, and gasifier and power turbine vanes are fabricated.

Optimization of a vacuum chamber for vibration measurements.

PubMed

Danyluk, Mike; Dhingra, Anoop

2011-10-01

A 200 °C high vacuum chamber has been built to improve vibration measurement sensitivity. The optimized design addresses two significant issues: (i) vibration measurements under high vacuum conditions and (ii) use of design optimization tools to reduce operating costs. A test rig consisting of a cylindrical vessel with one access port has been constructed with a welded-bellows assembly used to seal the vessel and enable vibration measurements in high vacuum that are comparable with measurements in air. The welded-bellows assembly provides a force transmissibility of 0.1 or better at 15 Hz excitation under high vacuum conditions. Numerical results based on design optimization of a larger diameter chamber are presented. The general constraints on the new design include material yield stress, chamber first natural frequency, vibration isolation performance, and forced convection heat transfer capabilities over the exterior of the vessel access ports. Operating costs of the new chamber are reduced by 50% compared to a preexisting chamber of similar size and function.

Advanced Gas Turbine (AGT) Technology Development Project

NASA Technical Reports Server (NTRS)

1987-01-01

This report is the eleventh in the series of Technical Summary reports for the Advanced Gas Turbine (AGT) Technology Development Project, authorized under NASA Contract DEN3-167, and sponsored by the Department of Energy (DOE). This report was prepared by Garrett Turbine Engine Company, A Division of the Garrett Corporation, and includes information provided by Ford Motor Company, the Standard Oil Company, and AiResearch Casting Company. This report covers plans and progress for the period July 1, 1985 through June 30, 1986. Technical progress during the reported period was highlighted by the 85-hour endurance run of an all-ceramic engine operating in the 2000 to 2250 F temperature regime. Component development continued in the areas of the combustion/fuel injection system, regenerator and seals system, and ceramic turbine rotor attachment design. Component rig testing saw further refinements. Ceramic materials showed continued improvements in required properties for gas turbine applications; however, continued development is needed before performance and reliability goals can be set.

Establish a Data Transmission Platform of the Rig Based on the Distributed Network

NASA Astrophysics Data System (ADS)

Bao, Zefu; Li, Tao

In order to control in real-time ,closed-loop feedback the information, saving the money and labor,we distribute a platform of network data. It through the establishment of the platform in the oil drilling to achieve the easiest route of each device of the rig that conveying timely. The design proposed the platform to transfer networking data by PA which allows the rig control for optimal use. Against the idea,achieving first through on-site cabling and the establishment of data transmission module in the rig monitoring system. The results of standard field application show that the platform solve the problem of rig control.

An implantable centrifugal blood pump with a recirculating purge system (Cool-Seal system).

PubMed

Yamazaki, K; Litwak, P; Tagusari, O; Mori, T; Kono, K; Kameneva, M; Watach, M; Gordon, L; Miyagishima, M; Tomioka, J; Umezu, M; Outa, E; Antaki, J F; Kormos, R L; Koyanagi, H; Griffith, B P

1998-06-01

A compact centrifugal blood pump has been developed as an implantable left ventricular assist system. The impeller diameter is 40 mm, and pump dimensions are 55 x 64 mm. This first prototype, fabricated from titanium alloy, resulted in a pump weight of 400 g including a brushless DC motor. The weight of a second prototype pump was reduced to 280 g. The entire blood contacting surface is coated with diamond like carbon (DLC) to improve blood compatibility. Flow rates of over 7 L/min against 100 mm Hg pressure at 2,500 rpm with 9 W total power consumption have been measured. A newly designed mechanical seal with a recirculating purge system (Cool-Seal) is used for the shaft seal. In this seal system, the seal temperature is kept under 40 degrees C to prevent heat denaturation of blood proteins. Purge fluid also cools the pump motor coil and journal bearing. Purge fluid is continuously purified and sterilized by an ultrafiltration unit which is incorporated in the paracorporeal drive console. In vitro experiments with bovine blood demonstrated an acceptably low hemolysis rate (normalized index of hemolysis = 0.005 +/- 0.002 g/100 L). In vivo experiments are currently ongoing using calves. Via left thoracotomy, left ventricular (LV) apex descending aorta bypass was performed utilizing an expanded polytetrafluoroethylene (ePTFE) vascular graft with the pump placed in the left thoracic cavity. In 2 in vivo experiments, the pump flow rate was maintained at 5-9 L/min, and pump power consumption remained stable at 9-10 W. All plasma free Hb levels were measured at less than 15 mg/dl. The seal system has demonstrated good seal capability with negligible purge fluid consumption (<0.5 ml/day). In both calves, the pumps demonstrated trouble free continuous function over 6 month (200 days and 222 days).

Novel approach for the refractive index gradient measurement in microliter volumes using fiber-optic technology

NASA Astrophysics Data System (ADS)

Synovec, Robert E.; Renn, Curtiss N.

1991-07-01

The refractive index gradient (RIG) of hydrodynamically controlled profiles can be universally, yet sensitively, measured by carefully probing the radial RIG passing through a z-configuration flow cell. Fiber optic technology is applied in order to provide a narrow, collimated probe beam (100 micrometers diameter) that is deflected by a RIG and measured by a position sensitive detector. The fiber optic construction allows one to probe very small volumes (1 (mu) L to 3 (mu) L) amenable to microbore liquid chromatography ((mu) LC). The combination of (mu) LC and RIG detection is very useful for the analysis of trace quantities (ng injected amounts) of chemical species that are generally difficult to measure, i.e., species that are not amenable to absorbance detection or related techniques. Furthermore, the RIG detector is compatible with conventional mobile phase gradient and thermal gradient (mu) LC, unlike traditional RI detectors. A description of the RIG detector coupled with (mu) LC for the analysis of complex polymer samples is reported. Also, exploration into using the RIG detector for supercritical fluid chromatography is addressed.

[Innate immune response to RNA virus infection].

PubMed

Oshiumi, Hiroyuki; Matsumoto, Misako; Seya, Tsukasa

2011-12-01

Viral RNA is recognized by RIG-I-like receptors and Toll-like receptors. RIG-I is a cytoplasmic viral RNA sensor. High Mobility Group Box (HMGB) proteins and DExD/H box RNA helicases, such as DDX3 and 60, associate with viral RNA. Those proteins promotes the RIG-I binding to viral RNA. RIG-I triggers the signal via IPS-1 adaptor molecule to induce type I IFN. RIG-I harbors Lys63-linked polyubiquitination by Riplet and TRIM25 ubiquitin ligases. The polyubiquitination is essential for RIG-I-mediated signaling. Toll-like receptors are located in endosome. TLR3 recognizes viral double-stranded RNA, and TLR7 and 8 recognize single-strand RNA. Virus has the ability to suppress these innate immune response. For example, to inhibit RIG-I-mediated signaling, HCV core protein suppresses the function of DDX3. In addition, HCV NS3-4A protein cleaves IPS-1 to inhibit the signal. Molecular mechanism of how viral RNA is recognized by innate immune system will make great progress on our understanding of how virus escapes from host immune system.

Structural and functional insights into 5'-ppp RNA pattern recognition by the innate immune receptor RIG-I.

PubMed

Wang, Yanli; Ludwig, Janos; Schuberth, Christine; Goldeck, Marion; Schlee, Martin; Li, Haitao; Juranek, Stefan; Sheng, Gang; Micura, Ronald; Tuschl, Thomas; Hartmann, Gunther; Patel, Dinshaw J

2010-07-01

RIG-I is a cytosolic helicase that senses 5'-ppp RNA contained in negative-strand RNA viruses and triggers innate antiviral immune responses. Calorimetric binding studies established that the RIG-I C-terminal regulatory domain (CTD) binds to blunt-end double-stranded 5'-ppp RNA a factor of 17 more tightly than to its single-stranded counterpart. Here we report on the crystal structure of RIG-I CTD bound to both blunt ends of a self-complementary 5'-ppp dsRNA 12-mer, with interactions involving 5'-pp clearly visible in the complex. The structure, supported by mutation studies, defines how a lysine-rich basic cleft within the RIG-I CTD sequesters the observable 5'-pp of the bound RNA, with a stacked phenylalanine capping the terminal base pair. Key intermolecular interactions observed in the crystalline state are retained in the complex of 5'-ppp dsRNA 24-mer and full-length RIG-I under in vivo conditions, as evaluated from the impact of binding pocket RIG-I mutations and 2'-OCH(3) RNA modifications on the interferon response.

Mitochondrially localised MUL1 is a novel modulator of antiviral signaling.

PubMed

Jenkins, Kristie; Khoo, Jing Jing; Sadler, Anthony; Piganis, Rebecca; Wang, Die; Borg, Natalie A; Hjerrild, Kathryn; Gould, Jodee; Thomas, Belinda J; Nagley, Phillip; Hertzog, Paul J; Mansell, Ashley

2013-04-01

The innate immune response to virus must be balanced to eliminate infection yet limit damaging inflammation. A critical arm of the antiviral response is launched by the retinoic acid-inducible-gene I (RIG-I) protein. RIG-I is activated by viral RNA then associates with the mitochondrial antiviral signaling (MAVS) protein to subsequently induce potent inflammatory cytokines. Here, we demonstrate the mitochondrial E3 ubiquitin protein ligase 1 (MUL1) is a crucial moderator of RIG-I signaling. MUL1 is localized to the mitochondria where it interacts with MAVS and catalyzes RIG-I post-translational modifications that inhibit RIG-I-dependent cell signaling. Accordingly, depletion of MUL1 potentiated RIG-I mediated nuclear factor-kappa B (NF-κB) and interferon (IFN) β reporter activity. Moreover, depletion of MUL1 boosted the antiviral response and increased proinflammatory cytokines following challenge with the RNA mimetic poly I:C and Sendai virus. We therefore submit that MUL1 is a novel regulator of the RIG-I-like receptor-dependent antiviral response, that otherwise functions to limit inflammation.

Theory versus experiment for the rotordynamic coefficients of annular gas seals. Part 1: Test facility and apparatus

NASA Technical Reports Server (NTRS)

Childs, D. W.; Nelson, C. E.; Nicks, C.; Scharrer, J. K.; Elrod, D.; Hale, K.

1983-01-01

A facility and apparatus are described for determining the rotordynamic coefficients and leakage characteristics of annular gas seals. The apparatus has a current top speed of 8000 cpm with a nominal seal diameter of 15.24 cmn (6 in). The air supply unit yields a seal pressure ratio of approximately 7. An external shaker is used to excite the test rotor. The capability to independently calculate all rotordynamic coefficients at a given operating condition with one excitation frequency are discussed.

Validation of numerical models for flow simulation in labyrinth seals

NASA Astrophysics Data System (ADS)

Frączek, D.; Wróblewski, W.

2016-10-01

CFD results were compared with the results of experiments for the flow through the labyrinth seal. RANS turbulence models (k-epsilon, k-omega, SST and SST-SAS) were selected for the study. Steady and transient results were analyzed. ANSYS CFX was used for numerical computation. The analysis included flow through sealing section with the honeycomb land. Leakage flows and velocity profiles in the seal were compared. In addition to the comparison of computational models, the divergence of modeling and experimental results has been determined. Tips for modeling these problems were formulated.

Destroyer Engineered Operating Cycle (DDEOC). System Maintenance Analysis CG-16 and CG-26 Class 1200 PSI Propulsion Plant SWAB Group 200 SMA 1626-200. Review of Experience,

DTIC Science & Technology

1979-11-01

shaft seals during the CASREP data period. Five of these CASREPs reported excessive leakage , two reported ruptured inflatable seals , and one reported...failures, like the DDG-37 Class CASREPs, were related to lube oil problems, steam valve problems, or leaking packing ( steam or oil seals ) that had become...sources of this water: (1) gradual deterioration of the turbine labyrinth seals , (2) leaking of exhaust and relief valves, and (3) condensation in the

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bradonjic, Milan; Hagberg, Aric; Hengartner, Nick

We analyze component evolution in general random intersection graphs (RIGs) and give conditions on existence and uniqueness of the giant component. Our techniques generalize the existing methods for analysis on component evolution in RIGs. That is, we analyze survival and extinction properties of a dependent, inhomogeneous Galton-Watson branching process on general RIGs. Our analysis relies on bounding the branching processes and inherits the fundamental concepts from the study on component evolution in Erdos-Renyi graphs. The main challenge becomes from the underlying structure of RIGs, when the number of offsprings follows a binomial distribution with a different number of nodes andmore » different rate at each step during the evolution. RIGs can be interpreted as a model for large randomly formed non-metric data sets. Besides the mathematical analysis on component evolution, which we provide in this work, we perceive RIGs as an important random structure which has already found applications in social networks, epidemic networks, blog readership, or wireless sensor networks.« less

Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I

DOE Office of Scientific and Technical Information (OSTI.GOV)

Devarkar, Swapnil C.; Wang, Chen; Miller, Matthew T.

The cytosolic innate immune receptor Retinoic Acid Inducible Gene-I (RIG-I) is the principal detector of pathogenic RNAs carrying a 5'-triphosphate (5'ppp). Self RNAs like mRNAs evade recognition by RIG-I due to posttranscriptional modifications like 5'-end capping with 7-methyl guanosine (m7G) and 2'-O-methylation of 5'-end nucleotides. Viruses have also evolved mechanisms to mimic these modifications, which in part is believed to aid in immune evasion. Currently, it is unclear how these modifications modulate RIG-I recognition. This paper provides structural and mechanistic insights into the roles of the m7G cap and 2'-O-methylation in RIG-I evasion. We show that RIG-I accommodates the m7Gmore » base while maintaining the 5'ppp contacts and can recognize Cap-0 RNAs but not Cap-1.« less

Long Term Outcome after Application of the Angio-Seal Vascular Closure Device in Minipigs

PubMed Central

Kabelitz, Lisa; Nonn, Andrea; Nolte, Kay W.; Nikoubashman, Omid; Othman, Ahmed; Heringer, Sarah; Kramer, Martin; Wiesmann, Martin; Brockmann, Marc A.

2016-01-01

Minipigs are frequently used in (neuro-)interventional research. Longitudinal experiments may require repeated vessel access via the femoral artery. Anticoagulation and incompliance of the animals necessitates the use of a vascular closure device (VCD). The effects of the Angio-Seal VCD in minipigs were longitudinally assessed. Minipig (42±8.4 kg body weight) femoral arteries were sealed using the 8F (n = 6) or 6F (n = 7) Angio-Seal VCD. The pre-interventional femoral artery diameter was 5.1±0.4 mm (4.3–5.8 mm). Sealed puncture sites were analysed angiographically as well as by computed tomography angiography (CTA) for a mean period of 14.1±8.0 weeks (1–22 weeks). All animals were constantly treated with acetylsalicylic acid (ASS) (450 mg/d (n = 7) or 100 mg/d (n = 1)) and clopidogrel (75 mg/d (n = 8)). Non-instrumented (n = 2) and arteries sealed using the VCD (n = 2) were examined histologically. No postoperative hemorrhagic complications were observed. Three arteries were occluded after VCD placement (1 animal diagnosed after 4 weeks (8F), 2 animals after 1 week (6F)) and remained so until the end of the experiments after 22, 12 and 4 weeks, respectively. In one artery a 50% stenosis 8 weeks after application of a 6F Angio-Seal was detected. In 69.2% (n = 9) the VCD was applied without complications. Histopathological analysis of the sealed arterial segments showed subtotal obliteration of the vessel lumen, formation of collagenous tissue and partial damage of the internal elastic lamina. The Angio-Seal VCD prevents relevant hemorrhagic complications in minipigs treated with dual platelet inhibition, but is associated with increased vessel occlusion rates. PMID:27682594

Numerical and Analytical Assessment of a Coupled Rotating Detonation Engine and Turbine Experiment

NASA Technical Reports Server (NTRS)

Paxson, Daniel E.; Naples, Andrew

2017-01-01

An analysis is presented of an experimental rig comprising a rotating detonation engine (RDE) with bypass ejector flow coupled to a downstream turbine. The analysis used a validated computational fluid dynamics RDE simulation combined with straightforward algebraic mixing equations for the bypass flow. The objectives of the analysis were to supplement and interpret the necessarily sparse measurements from the rig, and to assess the performance of the RDE itself, which was not instrumented in this installation. The analysis is seen to agree reasonably well with available data. It shows that the RDE is operating in an unusual fashion, with subsonic flow throughout the exhaust plane. The detonation event itself is producing a total pressure rise relative to the pre-detonative pressure; however, the length of the device and the substantial flow restriction at the inlet yield an overall pressure loss. This is not surprising since the objective of the rig test was primarily aimed at investigating RDE turbine interactions, and not on performance optimization. Furthermore, the RDE was designed for fundamental detonation studies and not performance. Nevertheless, the analysis indicates that with some small alterations to the design, an RDE with an overall pressure rise is possible.

Numerical and Analytical Assessment of a Coupled Rotating Detonation Engine and Turbine Experiment

NASA Technical Reports Server (NTRS)

Paxson, Daniel E.; Naples, Andrew

2017-01-01

An analysis is presented of an experimental rig comprising a rotating detonation engine (RDE) with bypass flow coupled to a downstream turbine. The analysis used a validated computational fluid dynamics RDE simulation combined with straightforward algebraic mixing equations for the bypass flow. The objectives of the analysis were to supplement and interpret the necessarily sparse measurements from the rig, and to assess the performance of the RDE itself (which was not instrumented in this installation). The analysis is seen to agree reasonably well with available data. It shows that the RDE is operating in an unusual fashion, with subsonic flow throughout the exhaust plane. The detonation event itself is producing a total pressure rise relative to the pre-detonative pressure; however, the length of the device and the substantial flow restriction at the inlet yield an overall pressure loss. This is not surprising since the objective of the rig test was primarily aimed at investigating RDEturbine interactions, and not on performance optimization. Furthermore, the RDE was designed for fundamental detonation studies and not performance. Nevertheless, the analysis indicates that with some small alterations to the design, an RDE with an overall pressure rise is possible.

Numerical and Analytical Assessment of a Coupled Rotating Detonation Engine and Turbine Experiment

NASA Technical Reports Server (NTRS)

Paxson, Daniel E.; Naples, Andrew

2017-01-01

An analysis is presented of an experimental rig comprising a rotating detonation engine (RDE) with bypass ejector flow coupled to a downstream turbine. The analysis used a validated computational fluid dynamics RDE simulation combined with straightforward algebraic mixing equations for the bypass flow. The objectives of the analysis were to supplement and interpret the necessarily sparse measurements from the rig, and to assess the performance of the RDE itself, which was not instrumented in this installation. The analysis is seen to agree reasonably well with available data. It shows that the RDE is operating in an unusual fashion, with subsonic flow throughout the exhaust plane. The detonation event itself is producing a total pressure rise relative to the pre-detonative pressure; however, the length of the device and the substantial flow restriction at the inlet yield an overall pressure loss. This is not surprising since the objective of the rig test was primarily aimed at investigating RDE/turbine interactions, and not on performance optimization. Furthermore, the RDE was designed for fundamental detonation studies and not performance. Nevertheless, the analysis indicates that with some small alterations to the design, an RDE with an overall pressure rise is possible.

Learning Inverse Rig Mappings by Nonlinear Regression.

PubMed

Holden, Daniel; Saito, Jun; Komura, Taku

2017-03-01

We present a framework to design inverse rig-functions-functions that map low level representations of a character's pose such as joint positions or surface geometry to the representation used by animators called the animation rig. Animators design scenes using an animation rig, a framework widely adopted in animation production which allows animators to design character poses and geometry via intuitive parameters and interfaces. Yet most state-of-the-art computer animation techniques control characters through raw, low level representations such as joint angles, joint positions, or vertex coordinates. This difference often stops the adoption of state-of-the-art techniques in animation production. Our framework solves this issue by learning a mapping between the low level representations of the pose and the animation rig. We use nonlinear regression techniques, learning from example animation sequences designed by the animators. When new motions are provided in the skeleton space, the learned mapping is used to estimate the rig controls that reproduce such a motion. We introduce two nonlinear functions for producing such a mapping: Gaussian process regression and feedforward neural networks. The appropriate solution depends on the nature of the rig and the amount of data available for training. We show our framework applied to various examples including articulated biped characters, quadruped characters, facial animation rigs, and deformable characters. With our system, animators have the freedom to apply any motion synthesis algorithm to arbitrary rigging and animation pipelines for immediate editing. This greatly improves the productivity of 3D animation, while retaining the flexibility and creativity of artistic input.

Robust Lys63-Linked Ubiquitination of RIG-I Promotes Cytokine Eruption in Early Influenza B Virus Infection.

PubMed

Jiang, Jingwen; Li, Jing; Fan, Wenhui; Zheng, Weinan; Yu, Meng; Chen, Can; Sun, Lei; Bi, Yuhai; Ding, Chan; Gao, George F; Liu, Wenjun

2016-07-15

Influenza A and B virus infections both cause a host innate immunity response. Here, we report that the robust production of type I and III interferons (IFNs), IFN-stimulated genes, and proinflammatory factors can be induced by influenza B virus rather than influenza A virus infection in alveolar epithelial (A549) cells during early infection. This response is mainly dependent on the retinoic acid-inducible gene I (RIG-I)-mediated signaling pathway. Infection by influenza B virus promotes intense Lys63-linked ubiquitination of RIG-I, resulting in cytokine eruption. It is known that the influenza A virus NS1 protein (NS1-A) interacts with RIG-I and TRIM25 to suppress the activation of RIG-I-mediated signaling. However, the present results indicate that the influenza B virus NS1 protein (NS1-B) is unable to interact with RIG-I but engages in the formation of a RIG-I/TRIM25/NS1-B ternary complex. Furthermore, we demonstrate that the N-terminal RNA-binding domain (RBD) of NS1-B is responsible for interaction with TRIM25 and that this interaction blocks the inhibitory effect of the NS1-B C-terminal effector domain (TED) on RIG-I ubiquitination. Our findings reveal a novel mechanism for the host cytokine response to influenza B virus infection through regulatory interplay between host and viral proteins. Influenza B virus generally causes local mild epidemics but is occasionally lethal to individuals. Existing studies describe the broad characteristics of influenza B virus epidemiology and pathology. However, to develop better prevention and treatments for the disease, determining the concrete molecular mechanisms of pathogenesis becomes pivotal to understand how the host reacts to the challenge of influenza B virus. Thus, we aimed to characterize the host innate immune response to influenza B virus infection. Here, we show that vigorous Lys63-linked ubiquitination of RIG-I and cytokine eruption dependent on RIG-I-mediated signal transduction are induced by virus infection. Additionally, TRIM25 positively regulates RIG-I-mediated signaling by ablating the inhibitory function of NS1-B on RIG-I ubiquitination. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Medical evacuations from oil rigs off the Gulf Coast of the United States from 2008 to 2012: reasons and cost implications.

PubMed

Thibodaux, Donald P; Bourgeois, Robert M; Loeppke, Ronald R; Konicki, Doris L; Hymel, Pamela A; Dreger, Marianne

2014-07-01

To identify reasons for air medical evacuations from oil rigs/platforms. Retrospective review of data of medical calls from 102 rigs/platforms in the US Gulf Coast from 2008 through 2012 with specific analysis of medevacs. On average, 1609 total calls per year relating to illness or injury on the 102 oil rigs/platforms with 4% to 7% requiring medical air evacuation. On average, 77% of medevacs were for nonoccupational medical injury or illness. Illness, not occupational injuries, is identified as the major reason for medical evacuations from oil rigs. Heart disease is the leading cause of chronic health conditions resulting in a medevac.

Design Study of Wafer Seals for Future Hypersonic Vehicles

NASA Technical Reports Server (NTRS)

Dunlap, Patrick H.; Finkbeiner, Joshua R.; Steinetz, Bruce M.; DeMange, Jeffrey J.

2005-01-01

Future hypersonic vehicles require high temperature, dynamic seals in advanced hypersonic engines and on the vehicle airframe to seal the perimeters of movable panels, flaps, and doors. Current seals do not meet the demanding requirements of these applications, so NASA Glenn Research Center is developing improved designs to overcome these shortfalls. An advanced ceramic wafer seal design has shown promise in meeting these needs. Results from a design of experiments study performed on this seal revealed that several installation variables played a role in determining the amount of leakage past the seals. Lower leakage rates were achieved by using a tighter groove width around the seals, a higher seal preload, a tighter wafer height tolerance, and a looser groove length. During flow testing, a seal activating pressure acting behind the wafers combined with simulated vibrations to seat the seals more effectively against the sealing surface and produce lower leakage rates. A seal geometry study revealed comparable leakage for full-scale wafers with 0.125 and 0.25 in. thicknesses. For applications in which lower part counts are desired, fewer 0.25-in.-thick wafers may be able to be used in place of 0.125-in.-thick wafers while achieving similar performance. Tests performed on wafers with a rounded edge (0.5 in. radius) in contact with the sealing surface resulted in flow rates twice as high as those for wafers with a flat edge. Half-size wafers had leakage rates approximately three times higher than those for full-size wafers.

Toscana Virus NSs Protein Inhibits the Induction of Type I Interferon by Interacting with RIG-I

PubMed Central

Gori-Savellini, Gianni; Valentini, Melissa

2013-01-01

Toscana virus (TOSV) is a phlebovirus, of the Bunyaviridae family, that is responsible for central nervous system (CNS) injury in humans. Previous data have shown that the TOSV NSs protein is a gamma interferon (IFN-β) antagonist when transiently overexpressed in mammalian cells, inhibiting IRF-3 induction (G. Gori Savellini, F. Weber, C. Terrosi, M. Habjan, B. Martorelli, and M. G. Cusi, J. Gen. Virol. 92:71–79, 2011). In this study, we investigated whether an upstream sensor, which has a role in the signaling cascade leading to the production of type I IFN, was involved. We found a significant decrease in RIG-I protein levels in cells overexpressing TOSV NSs, suggesting that the nonstructural protein interacts with RIG-I and targets it for proteasomal degradation. In fact, the MG-132 proteasome inhibitor was able to restore IFN-β promoter activation in cells expressing NSs, demonstrating the existence of an evasion mechanism based on inhibition of the RIG-I sensor. Furthermore, a C-terminal truncated NSs protein (ΔNSs), although able to interact with RIG-I, did not affect the RIG-I-mediated IFN-β promoter activation, suggesting that the NSs domains responsible for RIG-I-mediated signaling and interaction with RIG-I are mapped on different regions. These results contribute to identify a novel mechanism for bunyaviruses by which TOSV NSs counteracts the early IFN response. PMID:23552410

Toscana virus NSs protein inhibits the induction of type I interferon by interacting with RIG-I.

PubMed

Gori-Savellini, Gianni; Valentini, Melissa; Cusi, Maria Grazia

2013-06-01

Toscana virus (TOSV) is a phlebovirus, of the Bunyaviridae family, that is responsible for central nervous system (CNS) injury in humans. Previous data have shown that the TOSV NSs protein is a gamma interferon (IFN-β) antagonist when transiently overexpressed in mammalian cells, inhibiting IRF-3 induction (G. Gori Savellini, F. Weber, C. Terrosi, M. Habjan, B. Martorelli, and M. G. Cusi, J. Gen. Virol. 92:71-79, 2011). In this study, we investigated whether an upstream sensor, which has a role in the signaling cascade leading to the production of type I IFN, was involved. We found a significant decrease in RIG-I protein levels in cells overexpressing TOSV NSs, suggesting that the nonstructural protein interacts with RIG-I and targets it for proteasomal degradation. In fact, the MG-132 proteasome inhibitor was able to restore IFN-β promoter activation in cells expressing NSs, demonstrating the existence of an evasion mechanism based on inhibition of the RIG-I sensor. Furthermore, a C-terminal truncated NSs protein (ΔNSs), although able to interact with RIG-I, did not affect the RIG-I-mediated IFN-β promoter activation, suggesting that the NSs domains responsible for RIG-I-mediated signaling and interaction with RIG-I are mapped on different regions. These results contribute to identify a novel mechanism for bunyaviruses by which TOSV NSs counteracts the early IFN response.

Selective habituation shapes acoustic predator recognition in harbour seals.

PubMed

Deecke, Volker B; Slater, Peter J B; Ford, John K B

2002-11-14

Predation is a major force in shaping the behaviour of animals, so that precise identification of predators will confer substantial selective advantages on animals that serve as food to others. Because experience with a predator can be lethal, early researchers studying birds suggested that predator recognition does not require learning. However, a predator image that can be modified by learning and experience will be advantageous in situations where cues associated with the predator are highly variable or change over time. In this study, we investigated the response of harbour seals (Phoca vitulina) to the underwater calls of different populations of killer whales (Orcinus orca). We found that the seals responded strongly to the calls of mammal-eating killer whales and unfamiliar fish-eating killer whales but not to the familiar calls of the local fish-eating population. This demonstrates that wild harbour seals are capable of complex acoustic discrimination and that they modify their predator image by selectively habituating to the calls of harmless killer whales. Fear in these animals is therefore focused on local threats by learning and experience.

A closed-chamber method to measure greenhouse gas fluxes from dry aquatic sediments

NASA Astrophysics Data System (ADS)

Lesmeister, Lukas; Koschorreck, Matthias

2017-06-01

Recent research indicates that greenhouse gas (GHG) emissions from dry aquatic sediments are a relevant process in the freshwater carbon cycle. However, fluxes are difficult to measure because of the often rocky substrate and the dynamic nature of the habitat. Here we tested the performance of different materials to seal a closed chamber to stony ground both in laboratory and field experiments. Using on-site material consistently resulted in elevated fluxes. The artefact was caused both by outgassing of the material and production of gas. The magnitude of the artefact was site dependent - the measured CO2 flux increased between 10 and 208 %. Errors due to incomplete sealing proved to be more severe than errors due to non-inert sealing material.Pottery clay as sealing material provided a tight seal between the chamber and the ground and no production of gases was detected. With this approach it is possible to get reliable gas fluxes from hard-substrate sites without using a permanent collar. Our test experiments confirmed that CO2 fluxes from dry aquatic sediments are similar to CO2 fluxes from terrestrial soils.

Structural basis of RNA recognition and activation by innate immune receptor RIG-I

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jiang, Fuguo; Ramanathan, Anand; Miller, Matthew T.

Retinoic-acid-inducible gene-I (RIG-I; also known as DDX58) is a cytoplasmic pathogen recognition receptor that recognizes pathogen-associated molecular pattern (PAMP) motifs to differentiate between viral and cellular RNAs. RIG-I is activated by blunt-ended double-stranded (ds)RNA with or without a 5'-triphosphate (ppp), by single-stranded RNA marked by a 5'-ppp and by polyuridine sequences. Upon binding to such PAMP motifs, RIG-I initiates a signalling cascade that induces innate immune defences and inflammatory cytokines to establish an antiviral state. The RIG-I pathway is highly regulated and aberrant signalling leads to apoptosis, altered cell differentiation, inflammation, autoimmune diseases and cancer. The helicase and repressor domainsmore » (RD) of RIG-I recognize dsRNA and 5'-ppp RNA to activate the two amino-terminal caspase recruitment domains (CARDs) for signalling. Here, to understand the synergy between the helicase and the RD for RNA binding, and the contribution of ATP hydrolysis to RIG-I activation, we determined the structure of human RIG-I helicase-RD in complex with dsRNA and an ATP analogue. The helicase-RD organizes into a ring around dsRNA, capping one end, while contacting both strands using previously uncharacterized motifs to recognize dsRNA. Small-angle X-ray scattering, limited proteolysis and differential scanning fluorimetry indicate that RIG-I is in an extended and flexible conformation that compacts upon binding RNA. These results provide a detailed view of the role of helicase in dsRNA recognition, the synergy between the RD and the helicase for RNA binding and the organization of full-length RIG-I bound to dsRNA, and provide evidence of a conformational change upon RNA binding. The RIG-I helicase-RD structure is consistent with dsRNA translocation without unwinding and cooperative binding to RNA. The structure yields unprecedented insight into innate immunity and has a broader impact on other areas of biology, including RNA interference and DNA repair, which utilize homologous helicase domains within DICER and FANCM.« less

Olson Mechanical and Heavy Rigging, Inc

DTIC Science & Technology

1989-08-01

neutral advisor with experience in public contract law and two construction experts. The Corps and Olson agreed to share the costs of the neutral... contract law . Unfortunately, he fell ill and was not able to participate. Turner then suggested Mr. Guy Randles of the law finn of Stoel, Rives, Boley...Grey, & Jones, who had extensive experience in government contract law . Turner called Randles, and found he was interested in serving as the neutral

Rotordynamic coefficients for stepped labyrinth gas seals

NASA Technical Reports Server (NTRS)

Scharrer, Joseph K.

1989-01-01

The basic equations are derived for compressible flow in a stepped labyrinth gas seal. The flow is assumed to be completely turbulent in the circumferential direction where the friction factor is determined by the Blasius relation. Linearized zeroth and first-order perturbation equations are developed for small motion about a centered position by an expansion in the eccentricity ratio. The zeroth-order pressure distribution is found by satisfying the leakage equation while the circumferential velocity distribution is determined by satisfying the momentum equations. The first order equations are solved by a separation of variables solution. Integration of the resultant pressure distribution along and around the seal defines the reaction force developed by the seal and the corresponding dynamic coefficients. The results of this analysis are presented in the form of a parametric study, since there are no known experimental data for the rotordynamic coefficients of stepped labyrinth gas seals. The parametric study investigates the relative rotordynamic stability of convergent, straight and divergent stepped labyrinth gas seals. The results show that, generally, the divergent seal is more stable, rotordynamically, than the straight or convergent seals. The results also show that the teeth-on-stator seals are not always more stable, rotordynamically, then the teeth-on-rotor seals as was shown by experiment by Childs and Scharrer (1986b) for a 15 tooth seal.

PGIM-01, Coleman at middeck locker with rigged plastic air duct

NASA Image and Video Library

1999-07-25

S93-E-5043 (24 July 1999) --- Astronaut Catherine G. (Cady) Coleman, mission specialist, checks the support system for the Plant Growth Investigations in Microgravity 1 (PGIM-1) experiment on Columbia's middeck. The photo was recorded with an electronic still camera (ESC) on Flight Day 2.

Testing the Fracture Behaviour of Chocolate

ERIC Educational Resources Information Center

Parsons, L. B.; Goodall, R.

2011-01-01

In teaching the materials science aspects of physics, mechanical behaviour is important due to its relevance to many practical applications. This article presents a method for experimentally examining the toughness of chocolate, including a design for a simple test rig, and a number of experiments that can be performed in the classroom. Typical…

LeRC in-house experimental research

NASA Technical Reports Server (NTRS)

Lyons, Valerie J.

1992-01-01

The topics covered include the following: LeRC in-house experimental research; combustion concepts; schedule for in-house experiments; lean premixed prevaporized combustion; comparisons of low NO(x) lean premixed/prevaporized data; rich burn/quick quench/lean burn (RQL); RQL combustion; fuel rich catalytic combustion; advanced diagnostics; and ceramic matrix liner test rig.

Deposition of Na2SO4 from salt-seeded combustion gases of a high velocity burner rig

NASA Technical Reports Server (NTRS)

Santoro, G. J.; Kohl, F. J.; Stearns, C. A.; Gokoglu, S. A.; Rosner, D. A.

1985-01-01

With a view to developing simulation criteria for the laboratory testing of high-temperature materials for gas turbine engines, the deposition rates of sodium sulfate from sodium salt-seeded combustion gases were determined experimentally using a well instrumented high-velocity burner. In the experiments, Na2SO4, NaCl, NaNO3, and simulated sea salt solutions were injected into the combustor of the Mach 0.3 burner rig operating at constant fuel/air ratios. The deposits formed on an inert rotating collector were then weighed and analyzed. The experimental results are compared to Rosner's vapor diffusion theory. Some additional test results, including droplet size distribution of an atomized salt spray, are used in interpreting the deposition rate data.

Making Ice Creep in the Classroom

NASA Astrophysics Data System (ADS)

Prior, David; Vaughan, Matthew; Banjan, Mathilde; Hamish Bowman, M.; Craw, Lisa; Tooley, Lauren; Wongpan, Pat

2017-04-01

Understanding the creep of ice has direct application to the role of ice sheet flow in sea level and climate change and to modelling of icy planets and satellites of the outer solar system. Additionally ice creep can be used as an analogue for the high temperature creep of rocks, most particularly quartzites. We adapted technologies developed for ice creep experiments in the research lab, to build some inexpensive ( EU200) rigs to conduct ice creep experiments in an undergraduate (200 and 300 level) class in rock deformation. The objective was to give the students an experience of laboratory rock deformation experiments so that they would understand better what controls the creep rate of ice and rocks. Students worked in eight groups of 5/6 students. Each group had one deformation rig and temperature control system. Each group conducted two experiments over a 2 week period. The results of all 16 experiments were then shared so that all students could analyse the mechanical data and generate a "flow law" for ice. Additionally thin sections were made of each deformed sample so that some microstructural analysis could be incorporated in the data analysis. Students were able to derive a flow law that showed the relationship of creep rate to both stress and temperature. The flow law matches with those from published research. The class did provide a realistic introduction to laboratory rock deformation experiments and helped students' understanding of what controls the creep of rocks.

Linear ubiquitin assembly complex negatively regulates RIG-I and TRIM25 mediated type-I interferon induction

PubMed Central

Inn, Kyung-Soo; Gack, Michaela U.; Tokunaga, Fuminori; Shi, Mude; Wong, Lai-Yee; Iwai, Kazuhiro; Jung, Jae U.

2011-01-01

Summary Upon detection of viral RNA, retinoic acid inducible gene I (RIG-I) undergoes TRIM25-mediated Lys-63 linked ubiquitination, leading to type-I interferon (IFN) production. In this study, we demonstrate that the linear ubiquitin assembly complex (LUBAC), comprised of two RING-IBR-RING (RBR)-containing E3 ligases HOIL-1L and HOIP, independently targets TRIM25 and RIG-I to effectively suppress virus-induced IFN production. RBR E3 ligase domains of HOIL-1L and HOIP bind and induce proteosomal degradation of TRIM25, whereas the NZF domain of HOIL-1L competes with TRIM25 for RIG-I binding. Consequently, both actions by the HOIL-1L/HOIP LUBAC potently inhibit RIG-I ubiquitination and anti-viral activity, but in a mechanistically separate manner. Conversely, the genetic deletion or depletion of HOIL-1L and HOIP robustly enhances virus-induced type-I IFN production. Taken together, the HOIL-1L/HOIP LUBAC specifically suppresses RIG-I ubiquitination and activation by inducing TRIM25 degradation and inhibiting TRIM25 interaction with RIG-I, resulting in the comprehensive suppression of the IFN-mediated anti-viral signaling pathway. PMID:21292167

Linear ubiquitin assembly complex negatively regulates RIG-I- and TRIM25-mediated type I interferon induction.

PubMed

Inn, Kyung-Soo; Gack, Michaela U; Tokunaga, Fuminori; Shi, Mude; Wong, Lai-Yee; Iwai, Kazuhiro; Jung, Jae U

2011-02-04

Upon detection of viral RNA, retinoic acid-inducible gene I (RIG-I) undergoes TRIM25-mediated K63-linked ubiquitination, leading to type I interferon (IFN) production. In this study, we demonstrate that the linear ubiquitin assembly complex (LUBAC), comprised of two RING-IBR-RING (RBR)-containing E3 ligases, HOIL-1L and HOIP, independently targets TRIM25 and RIG-I to effectively suppress virus-induced IFN production. RBR E3 ligase domains of HOIL-1L and HOIP bind and induce proteasomal degradation of TRIM25, whereas the NZF domain of HOIL-1L competes with TRIM25 for RIG-I binding. Consequently, both actions by the HOIL-1L/HOIP LUBAC potently inhibit RIG-I ubiquitination and antiviral activity, but in a mechanistically separate manner. Conversely, the genetic deletion or depletion of HOIL-1L and HOIP robustly enhances virus-induced type I IFN production. Taken together, the HOIL-1L/HOIP LUBAC specifically suppresses RIG-I ubiquitination and activation by inducing TRIM25 degradation and inhibiting TRIM25 interaction with RIG-I, resulting in the comprehensive suppression of the IFN-mediated antiviral signaling pathway. Copyright © 2011 Elsevier Inc. All rights reserved.

Insight into Buffalo (Bubalus bubalis) RIG1 and MDA5 Receptors: A Comparative Study on dsRNA Recognition and In-Vitro Antiviral Response

PubMed Central

Singh, Manvender; Brahma, Biswajit; Maharana, Jitendra; Patra, Mahesh Chandra; Kumar, Sushil; Mishra, Purusottam; Saini, Megha; De, Bidhan Chandra; Mahanty, Sourav; Datta, Tirtha Kumar; De, Sachinandan

2014-01-01

RIG1 and MDA5 have emerged as important intracellular innate pattern recognition receptors that recognize viral RNA and mediate cellular signals controlling Type I interferon (IFN-I) response. Buffalo RIG1 and MDA5 genes were investigated to understand the mechanism of receptor induced antiviral response. Sequence analysis revealed that RIG1 and MDA5 maintain a domain arrangement that is common in mammals. Critical binding site residues of the receptors are evolutionary conserved among mammals. Molecular dynamics simulations suggested that RIG1 and MDA5 follow a similar, if not identical, dsRNA binding pattern that has been previously reported in human. Moreover, binding free energy calculation revealed that MDA5 had a greater affinity towards dsRNA compared to RIG1. Constitutive expressions of RLR genes were ubiquitous in different tissues without being specific to immune organs. Poly I:C stimulation induced elevated expressions of IFN-β and IFN-stimulated genes (ISGs) through interferon regulatory factors (IRFs) mediated pathway in buffalo foetal fibroblast cells. The present study provides crucial insights into the structure and function of RIG1 and MDA5 receptors in buffalo. PMID:24587036

Rich burn combustor technology at Pratt and Whitney

NASA Technical Reports Server (NTRS)

Lohmann, Robert P.; Rosfjord, T. J.

1992-01-01

The topics covered include the following: near term objectives; rich burn quick quench combustor (RBQC); RBQC critical technology areas; cylindrical RBQQ combustor rig; modular RBQQ combustor; cylindrical rig objectives; quench zone mixing; noneffusive cooled liner; variable geometry requirements; and sector combustor rig.

Crack sealing before overlaying an asphalt concrete surface.

DOT National Transportation Integrated Search

1997-01-01

In an attempt to reduce reflective cracking on overlay projects a study was conducted that : consisted of sealing all transverse cracks greater than inch with hot crack sealers before the : project was resurfaced. The experiment will evaluate the ...

Temporal and spatial patterns of habitat use by juveniles of a small coastal shark (Mustelus lenticulatus) in an estuarine nursery.

PubMed

Francis, Malcolm P

2013-01-01

Juvenile rig (Mustelus lenticulatus) were internally tagged with acoustic transmitters and tracked with acoustic receivers deployed throughout two arms of Porirua Harbour, a small (7 km(2)) estuary in New Zealand. Ten rig were tracked for up to four months during summer-autumn to determine their spatial and temporal use of the habitat. The overall goal was to estimate the size of Marine Protected Areas required to protect rig nursery areas from direct human impacts. Rig showed clear site preferences, but those preferences varied among rig and over time. They spent most of their time in large basins and on shallow sand and mud flats around the margins, and avoided deep channels. Habitat range increased during autumn for many of the rig. Only one shark spent time in both harbour arms, indicating that there was little movement between the two. Rig home ranges were 2-7 km(2), suggesting that an effective MPA would need to cover the entire Porirua Harbour. They moved to outer harbour sites following some high river flow rates, and most left the harbour permanently during or soon after a river spike, suggesting that they were avoiding low salinity water. Rig showed strong diel movements during summer, although the diel pattern weakened in autumn. Persistent use of the same day and night sites indicates that diel movements are directed rather than random. Further research is required to determine the sizes of rig home ranges in larger harbours where nursery habitat is more extensive. Marine Protected Areas do not control land-based impacts such as accelerated sedimentation and heavy metal pollution, so integration of marine and terrestrial management tools across a range of government agencies is essential to fully protect nursery areas.

Low Frequency Noise Contamination in Fan Model Testing

NASA Technical Reports Server (NTRS)

Brown, Clifford A.; Schifer, Nicholas A.

2008-01-01

Aircraft engine noise research and development depends on the ability to study and predict the noise created by each engine component in isolation. The presence of a downstream pylon for a model fan test, however, may result in noise contamination through pylon interactions with the free stream and model exhaust airflows. Additionally, there is the problem of separating the fan and jet noise components generated by the model fan. A methodology was therefore developed to improve the data quality for the 9 15 Low Speed Wind Tunnel (LSWT) at the NASA Glenn Research Center that identifies three noise sources: fan noise, jet noise, and rig noise. The jet noise and rig noise were then measured by mounting a scale model of the 9 15 LSWT model fan installation in a jet rig to simulate everything except the rotating machinery and in duct components of fan noise. The data showed that the spectra measured in the LSWT has a strong rig noise component at frequencies as high as 3 kHz depending on the fan and airflow fan exit velocity. The jet noise was determined to be significantly lower than the rig noise (i.e., noise generated by flow interaction with the downstream support pylon). A mathematical model for the rig noise was then developed using a multi-dimensional least squares fit to the rig noise data. This allows the rig noise to be subtracted or removed, depending on the amplitude of the rig noise relative to the fan noise, at any given frequency, observer angle, or nozzle pressure ratio. The impact of isolating the fan noise with this method on spectra, overall power level (OAPWL), and Effective Perceived Noise Level (EPNL) is studied.

Temporal and Spatial Patterns of Habitat Use by Juveniles of a Small Coastal Shark (Mustelus lenticulatus) in an Estuarine Nursery

PubMed Central

Francis, Malcolm P.

2013-01-01

Juvenile rig (Mustelus lenticulatus) were internally tagged with acoustic transmitters and tracked with acoustic receivers deployed throughout two arms of Porirua Harbour, a small (7 km2) estuary in New Zealand. Ten rig were tracked for up to four months during summer–autumn to determine their spatial and temporal use of the habitat. The overall goal was to estimate the size of Marine Protected Areas required to protect rig nursery areas from direct human impacts. Rig showed clear site preferences, but those preferences varied among rig and over time. They spent most of their time in large basins and on shallow sand and mud flats around the margins, and avoided deep channels. Habitat range increased during autumn for many of the rig. Only one shark spent time in both harbour arms, indicating that there was little movement between the two. Rig home ranges were 2–7 km2, suggesting that an effective MPA would need to cover the entire Porirua Harbour. They moved to outer harbour sites following some high river flow rates, and most left the harbour permanently during or soon after a river spike, suggesting that they were avoiding low salinity water. Rig showed strong diel movements during summer, although the diel pattern weakened in autumn. Persistent use of the same day and night sites indicates that diel movements are directed rather than random. Further research is required to determine the sizes of rig home ranges in larger harbours where nursery habitat is more extensive. Marine Protected Areas do not control land-based impacts such as accelerated sedimentation and heavy metal pollution, so integration of marine and terrestrial management tools across a range of government agencies is essential to fully protect nursery areas. PMID:23437298

Five-Axis, Three-Magnetic-Bearing Dynamic Spin Rig

NASA Technical Reports Server (NTRS)

Morrison, Carlos R.; Provenza, Andrew; Kurkov, Anatole; Mehmed, Oral; Johnson, Dexter; Montague, Gerald; Duffy, Kirsten; Jansen, Ralph

2005-01-01

The Five-Axis, Three-Magnetic-Bearing Dynamic Spin Rig is an apparatus for vibration testing of turbomachine blades in a vacuum at rotational speeds from 0 to 40,000 rpm. This rig includes (1) a vertically oriented shaft on which is mounted an assembly comprising a rotor holding the blades to be tested, (2) two actively controlled heteropolar radial magnetic bearings at opposite ends of the shaft, and (3) an actively controlled magnetic thrust bearing at the upper end of the shaft. This rig is a more capable successor to a prior apparatus, denoted the Dynamic Spin Rig (DSR), that included a vertically oriented shaft with a mechanical thrust bearing at the upper end and a single actively controlled heteropolar radial magnetic bearing at the lower end.

Cheating the Children.

ERIC Educational Resources Information Center

Rist, Marilee C.

1993-01-01

Schools are the victims, as bid-rigging scandals in the dairy-products industry surface in 13 states. School boards, in close cooperation with superintendents and district business managers, have the responsibility to detect bid-rigging or collusion. Offers guides for good business management and warning signs of bid-rigging. (MLF)

New drilling rigs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tubb, M.

1983-03-01

Petronas recently commissioned its first offshore jack-up drilling rig at Promet, Singapore. The $49 million jack-up Parameswara will undertake both exploration and development activities in Petronas Carigali's exploration block off the eastern coast of Malaysia. The block measures 19,800 sq. km. Initially, the rig will be located at the Duyong gas field. Based on Baker Marine Corporation's BMC 300 design, the 65 X 64 X 8 m rig is capable of working in water depths of up to 91.4 m and is able to drill to a depth of 7,600 m. It has three triangular open-lattice truss-type legs, each 131more » m long. Prominent features include four-tier living quarters which can house 90 men, three cranes of boom length 30.48 m each, a helideck, mess hall, galley, and recreation room. The rig is built to American Bureau of Shipping standards. This paper describes the transport, installation and ballast operations involved in situating the Petronas rig in the Duyong field.« less

Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway

PubMed Central

Sanchez, Jacint G.; Chiang, Jessica J.; Sparrer, Konstantin M.J.; Alam, Steven L.; Chi, Michael; Roganowicz, Marcin D.; Sankaran, Banumathi; Gack, Michaela U.; Pornillos, Owen

2016-01-01

SUMMARY Antiviral response pathways induce interferon by higher-order assembly of signaling complexes called signalosomes. Assembly of the RIG-I signalosome is regulated by K63-linked polyubiquitin chains, which are synthesized by the E3 ubiquitin ligase, TRIM25. We have previously shown that the TRIM25 coiled-coil domain is a stable, antiparallel dimer that positions two catalytic RING domains on opposite ends of an elongated rod. We now show that the RING domain is a separate self-association motif that engages ubiquitin-conjugated E2 enzymes as a dimer. RING dimerization is required for catalysis, TRIM25-mediated RIG-I ubiquitination, interferon induction, and antiviral activity. We also provide evidence that RING dimerization and E3 ligase activity are promoted by binding of the TRIM25 SPRY domain to the RIG-I effector domain. These results indicate that TRIM25 actively participates in higher-order assembly of the RIG-I signalosome and helps to fine-tune the efficiency of the RIG-I-mediated antiviral response. PMID:27425606

Multi-Axis Test Facility

NASA Image and Video Library

1959-11-01

Multi-Axis Test Facility, Space Progress Report, November 1, 1959: The Multi Axis Space Test Inertia Facility [MASTIF], informally referred to as the Gimbal Rig, was installed inside the Altitude Wind Tunnel. The rig, which spun on three axis simultaneously, was used to train the Mercury astronauts on how to bring a spinning spacecraft under control and to determine the effects of rapid spinning on the astronaut's eyesight and psyche. Small gaseous nitrogen jets were operated by the pilot to gain control of the rig after it had been set in motion. Part 1 shows pilot Joe Algranti in the rig as it rotates over one, two, and three axis. It also has overall views of the test set-up with researchers and technicians on the test platform. Part 2 shows Algranti being secured in the rig prior to the test. The rig is set in motion and the pilot slowly brings it under control. The Mercury astronauts trained on the MASTIF in early spring of 1960.

Effects of Compression, Staging, and Braid Angle on Braided Rope Seal Performance

NASA Technical Reports Server (NTRS)

Steinetz, Bruce M.; Adams, Michael L.

1997-01-01

Future turbine engines and industrial systems will be operating at increased temperatures to achieve more demanding efficiency and performance goals. In the highest temperature sections of the engine new material systems such as ceramics and intermetallics are being considered to withstand the harsh thermal environment. Components constructed of these low expansion-rate materials experience thermal strains and a resulting reduction of life when rigidly attached to high expansion-rate, superalloy support structures. Seals are being designed to both seal and to serve as compliant mounts allowing for relative thermal growths between high temperature but brittle primary structures and the surrounding support structures. Previous seal research yielded several braided rope seal designs which demonstrated the ability to both seal and serve as a compliant mount. The hybrid seal was constructed of an all-ceramic (alumina-silica) core overbraided with a superalloy wire sheath (cobalt based superalloy). The all ceramic seal was constructed of an all-ceramic (alumina-silica) core overbraided with multiple ceramic (alumina-silica) sheath layers. Program goals for braided rope seals are to improve flow resistance and/or seal resilience. To that end, the current report studies the test results of: baseline and modified hybrid seals; two stage hybrid and two stage all-ceramic seal configurations; and single stage hybrid and single stage all-ceramic seal configurations for a range of seal crush conditions. Hybrid seal modifications include increasing the sheath braid angle and core coverage. For the same percent seal cross-sectional crush, results show that increasing the hybrid seal braid angle increased seal stiffness and seal unit load, resulting in flows approximately one third of the baseline hybrid seal flows. For both hybrid and all-ceramic seals, two stage seal configurations significantly outperformed single stage configurations. Two stage seal flows were at least 30% less than the single stage seal flows for the same seal crush. Furthermore, test results of single stage seals indicate that for both all-ceramic and hybrid seals, a specific seal crush condition exists at which minimum flows are achieved (i.e. increasing seal crush beyond a certain point does not result in better flow performance). Flow results are presented for a range of pressures and temperatures from ambient to 1300 F, before and after scrubbing. Compression tests results show that for both all-ceramic and hybrid seals, seal preload and stiffness increase with seal crush, but residual seal interference remains constant.

Turbomachine Sealing and Secondary Flows. Part 1; Review of Sealing Performance, Customer, Engine Designer, and Research Issues

NASA Technical Reports Server (NTRS)

Hendricks, R. C.; Steinetz, B. M.; Braun, M. J.

2004-01-01

Although forces outside our control shape our industry, turbomachine sealing research, design, and customer agendas established in 1978 by Ludwig, Campbell, and Smith in terms of specific fuel consumption and performance remain as objectives today. Advances have been made because failures of the space shuttle main engine turbomachinery ushered in a new understanding of sealing in high-power-density systems. Further, it has been shown that changes in sealing, especially for high-pressure rotors, dramatically change the performance of the entire engine or turbomachine. Maintaining seal leakages and secondary flows within engine design specifications remains the most efficient and cost effective way to enhance performance and minimize maintenance costs. This three-part review summarizes experiences, ideas, successes, and failures by NASA and the U.S. aerospace industry in secondary flow management in advanced turbomachinery. Part 1 presents system sealing, part 2 system rotordynamics, and part 3 modeling, with some overlap of each part.

49 CFR 230.111 - Spring rigging.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Spring rigging. 230.111 Section 230.111... Tenders Trucks, Frames and Equalizing System § 230.111 Spring rigging. (a) Arrangement of springs and equalizers. Springs and equalizers shall be arranged to ensure the proper distribution of weight to the...

49 CFR 230.111 - Spring rigging.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Spring rigging. 230.111 Section 230.111... Tenders Trucks, Frames and Equalizing System § 230.111 Spring rigging. (a) Arrangement of springs and equalizers. Springs and equalizers shall be arranged to ensure the proper distribution of weight to the...

49 CFR 230.111 - Spring rigging.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false Spring rigging. 230.111 Section 230.111... Tenders Trucks, Frames and Equalizing System § 230.111 Spring rigging. (a) Arrangement of springs and equalizers. Springs and equalizers shall be arranged to ensure the proper distribution of weight to the...

49 CFR 230.111 - Spring rigging.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Spring rigging. 230.111 Section 230.111... Tenders Trucks, Frames and Equalizing System § 230.111 Spring rigging. (a) Arrangement of springs and equalizers. Springs and equalizers shall be arranged to ensure the proper distribution of weight to the...

The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling.

PubMed

Chiang, Cindy; Pauli, Eva-Katharina; Biryukov, Jennifer; Feister, Katharina F; Meng, Melissa; White, Elizabeth A; Münger, Karl; Howley, Peter M; Meyers, Craig; Gack, Michaela U

2018-03-15

Retinoic acid-inducible gene I (RIG-I) is a key pattern recognition receptor that senses viral RNA and interacts with the mitochondrial adaptor MAVS, triggering a signaling cascade that results in the production of type I interferons (IFNs). This signaling axis is initiated by K63-linked ubiquitination of RIG-I mediated by the E3 ubiquitin ligase TRIM25, which promotes the interaction of RIG-I with MAVS. USP15 was recently identified as an upstream regulator of TRIM25, stabilizing the enzyme through removal of degradative K48-linked polyubiquitin, ultimately promoting RIG-I-dependent cytokine responses. Here, we show that the E6 oncoprotein of human papillomavirus type 16 (HPV16) as well as of other HPV types form a complex with TRIM25 and USP15 in human cells. In the presence of E6, the K48-linked ubiquitination of TRIM25 was markedly increased, and in line with this, TRIM25 degradation was enhanced. Our results further showed that E6 inhibited the TRIM25-mediated K63-linked ubiquitination of RIG-I and its CARD-dependent interaction with MAVS. HPV16 E6, but not E7, suppressed the RIG-I-mediated induction of IFN-β, chemokines, and IFN-stimulated genes (ISGs). Finally, CRISPR-Cas9 gene targeting in human keratinocytes showed that the TRIM25-RIG-I-MAVS triad is important for eliciting an antiviral immune response to HPV16 infection. Our study thus identifies a novel immune escape mechanism that is conserved among different HPV strains and further indicates that the RIG-I signaling pathway plays an important role in the innate immune response to HPV infection. IMPORTANCE Persistent infection and tumorigenesis by HPVs are known to require viral manipulation of a variety of cellular processes, including those involved in innate immune responses. Here, we show that the HPV E6 oncoprotein antagonizes the activation of the cytoplasmic innate immune sensor RIG-I by targeting its upstream regulatory enzymes TRIM25 and USP15. We further show that the RIG-I signaling cascade is important for an antiviral innate immune response to HPV16 infection, providing evidence that RIG-I, whose role in sensing RNA virus infections has been well characterized, also plays a crucial role in the antiviral host response to small DNA viruses of the Papillomaviridae family. Copyright © 2018 American Society for Microbiology.

Experimental impact testing and analysis of composite fan cases

NASA Astrophysics Data System (ADS)

Vander Klok, Andrew Joe

For aircraft engine certification, one of the requirements is to demonstrate the ability of the engine to withstand a fan blade-out (FBO) event. A FBO event may be caused by fatigue failure of the fan blade itself or by impact damage of foreign objects such as bird strike. An un-contained blade can damage flight critical engine components or even the fuselage. The design of a containment structure is related to numerous parameters such as the blade tip speed; blade material, size and shape; hub/tip diameter; fan case material, configuration, rigidity, etc. To investigate all parameters by spin experiments with a full size rotor assembly can be prohibitively expensive. Gas gun experiments can generate useful data for the design of engine containment cases at much lower costs. To replicate the damage modes similar to that on a fan case in FBO testing, the gas gun experiment has to be carefully designed. To investigate the experimental procedure and data acquisition techniques for FBO test, a low cost, small spin rig was first constructed. FBO tests were carried out with the small rig. The observed blade-to-fan case interactions were similar to those reported using larger spin rigs. The small rig has the potential in a variety of applications from investigating FBO events, verifying concept designs of rotors, to developing spin testing techniques. This rig was used in the developments of the notched blade releasing mechanism, a wire trigger method for synchronized data acquisition, high speed video imaging and etc. A relationship between the notch depth and the release speed was developed and verified. Next, an original custom designed spin testing facility was constructed. Driven by a 40HP, 40,000rpm air turbine, the spin rig is housed in a vacuum chamber of phi72inx40in (1829mmx1016mm). The heavily armored chamber is furnished with 9 viewports. This facility enables unprecedented investigations of FBO events. In parallel, a 15.4ft (4.7m) long phi4.1inch (105mm) diameter single stage gas gun was developed. A thermodynamic based relationship between the required gas pressure and targeted velocity was proposed. The predicted velocity was within +/-7%. Quantitative measurements of force and displacement were attempted. The transmitted impact force was measured with load cells. The out-of-plane deformation was measured with a projection grating profilometry method. The composite panels and fan cases used in this work were made of S2-glass plain weave fabrics with API SC-15 toughened epoxy resin using the vacuum assisted resin transfer molding (VARTM) method. Using the gas gun, the impact behavior of the composite was investigated at velocities ranging from 984ft/s to 1502ft/s (300m/s to 458m/s) following a draft ASTM testing standard. To compare the ballistic protection capability of different materials, a new parameter EBL, the projectile kinetic energy at the target ballistic limit normalized by the contact area of the projectile, was proposed. S2-glass/epoxy composite is ranked very high in EBL per areal weight. Finally, a testing method for replicating spin pit testing with a gas gun test was developed. Major differences between the two tests are the initial conditions of the blade upon contact with the target. In spin testing, the released blade has two velocity components, rotational and translational whereas in gas gun testing, the projectile has only the translational velocity. To account for the influence of the rotational velocity, three projectile designs were experimentally investigated. The results show that to generate similar damage modes in gas gun testing, it is critical to ensure the deformation of the projectile before testing is similar to that of a released blade. With the pre-bent blade, the gas gun experiment was able to replicate the damage modes of the fan case in FBO test on flat composite panels.

DIME Participants Prepare Experiment Rig

NASA Technical Reports Server (NTRS)

2002-01-01

Students prepare their experiment apparatus for free-fall during the second Dropping in a Microgravity Environment (DIME) competition held April 23-25, 2002, at NASA's Glenn Research Center. Competitors included two teams from Sycamore High School, Cincinnati, OH, and one each from Bay High School, Bay Village, OH, and COSI Academy, Columbus, OH. DIME is part of NASA's education and outreach activities. Details are on line at http://microgravity.grc.nasa.gov/DIME_2002.html.

DIME Participant Preparing Experiment Rig

NASA Technical Reports Server (NTRS)

2002-01-01

Students prepare to load fluids in their experiment apparatus during the Dropping In a Microgravity Environment (DIME) competition held April 23-25, 2002, at NASA's Glenn Research Center. Competitors included two teams from Sycamore High School, Cincinnati, OH, and one each from Bay High School, Bay Village, OH, and COSI Academy, Columbus, OH. DIME is part of NASA's education and outreach activities. Details are on line at http://microgravity.grc.nasa.gov/DIME_2002.html.

A durable, non power consumptive, simple seal for rotary blood pumps.

PubMed

Mitamura, Y; Sekine, K; Asakawa, M; Yozu, R; Kawada, S; Okamoto, E

2001-01-01

One of the key technologic requirements for rotary blood pumps is the sealing of the motor shaft. A mechanical seal, a journal bearing, magnetic coupling, and magnetic suspension have been developed, but they have drawbacks such as wear, thrombus formation, and power consumption. A magnetic fluid seal was developed for an axial flow pump. A magnetic fluid seal is durable, simple, and non power consumptive. Long-term experiments and finite element modeling (FEM) analyses confirmed these advantages. The seal body was composed of a Ned-Fe magnet and two pole pieces; the seal was formed by injecting ferrofluid into the gap (50 microm) between the pole pieces and the motor shaft. To contain the ferrofluid in the seal and to minimize the possibility of ferrofluid making contact with blood, a shield with a small cavity was attached to the pole piece. While submerged in blood, the sealing pressure of the seal was measured and found to be 188 mm Hg with ferrofluid LS-40 (saturated magnetization, 24.3 kA/m) at a motor speed of 10,000 rpm and 225 mm Hg under static conditions. The magnetic fluid seals performed perfectly at a pressure of 100 mm Hg for 594 + days in a static condition, and 51, 39+, and 34+ days at a motor speed of 8,000 rpm. FEM analyses indicated a theoretical sealing pressure of 260 mm Hg. The state of the magnetic fluid in the seal in water was observed with a microscope. Neither splashing of magnetic fluid nor mixing of the magnetic fluid and water was observed. The specially designed magnetic fluid seal for keeping liquids out is useful for axial flow blood pumps. The magnetic fluid seal was incorporated into an intracardiac axial flow pump.

Time-Dependent Testing Evaluation and Modeling for Rubber Stopper Seal Performance.

PubMed

Zeng, Qingyu; Zhao, Xia

2018-01-01

Sufficient rubber stopper sealing performance throughout the entire sealed product life cycle is essential for maintaining container closure integrity in the parenteral packaging industry. However, prior publications have lacked systematic considerations for the time-dependent influence on sealing performance that results from the viscoelastic characteristics of the rubber stoppers. In this paper, we report results of an effort to study these effects by applying both compression stress relaxation testing and residual seal force testing for time-dependent experimental data collection. These experiments were followed by modeling fit calculations based on the Maxwell-Wiechert theory modified with the Kohlrausch-Williams-Watts stretched exponential function, resulting in a nonlinear, time-dependent sealing force model. By employing both testing evaluations and modeling calculations, an in-depth understanding of the time-dependent effects on rubber stopper sealing force was developed. Both testing and modeling data show good consistency, demonstrating that the sealing force decays exponentially over time and eventually levels off because of the viscoelastic nature of the rubber stoppers. The nonlinearity of stress relaxation derives from the viscoelastic characteristics of the rubber stoppers coupled with the large stopper compression deformation into restrained geometry conditions. The modeling fit with capability to handle actual testing data can be employed as a tool to calculate the compression stress relaxation and residual seal force throughout the entire sealed product life cycle. In addition to being time-dependent, stress relaxation is also experimentally shown to be temperature-dependent. The present work provides a new, integrated methodology framework and some fresh insights to the parenteral packaging industry for practically and proactively considering, designing, setting up, controlling, and managing stopper sealing performance throughout the entire sealed product life cycle. LAY ABSTRACT: Historical publications in the parenteral packaging industry have lacked systematic considerations for the time-dependent influence on the sealing performance that results from effects of viscoelastic characteristic of the rubber stoppers. This study applied compression stress relaxation testing and residual seal force testing for time-dependent experimental data collection. These experiments were followed by modeling fit calculations based on the Maxwell-Wiechert theory modified with the Kohlrausch-Williams-Watts stretched exponential function, resulting in a nonlinear, time-dependent sealing force model. Experimental and modeling data show good consistency, demonstrating that sealing force decays exponentially over time and eventually levels off. The nonlinearity of stress relaxation derives from the viscoelastic characteristics of the rubber stoppers coupled with the large stopper compression deformation into restrained geometry conditions. In addition to being time-dependent stress relaxation, it is also experimentally shown to be temperature-dependent. The present work provides a new, integrated methodology framework and some fresh insights to the industry for practically and proactively considering, designing, setting up, controlling, and managing of the stopper sealing performance throughout the entire sealed product life cycle. © PDA, Inc. 2018.

Reconfigurable Image Generator

NASA Technical Reports Server (NTRS)

Archdeacon, John L. (Inventor); Iwai, Nelson H. (Inventor); Kato, Kenji H. (Inventor); Sweet, Barbara T. (Inventor)

2017-01-01

A RiG may simulate visual conditions of a real world environment, and generate the necessary amount of pixels in a visual simulation at rates up to 120 frames per second. RiG may also include a database generation system capable of producing visual databases suitable to drive the visual fidelity required by the RiG.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Suchanecki, Z.; Antoniou, I.; Tasaki, S.

We consider the problem of rigging for the Koopman operators of the Renyi and the baker maps. We show that the rigged Hilbert space for the Renyi maps has some of the properties of a strict inductive limit and give a detailed description of the rigged Hilbert space for the baker maps. {copyright} {ital 1996 American Institute of Physics.}

29 CFR 1926.1431 - Hoisting personnel.

Code of Federal Regulations, 2011 CFR

2011-07-01

... hook, load line and rigging) must not exceed 50 percent of the rated capacity for the radius and... pursuant to paragraph (b)(2) of this section, the total load (including the hook, load line, rigging and... number required to perform the work, whichever is less. (g) Attachment and rigging. (1) Hooks and other...

49 CFR 229.65 - Spring rigging.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false Spring rigging. 229.65 Section 229.65....65 Spring rigging. (a) Protective construction or safety hangers shall be provided to prevent spring planks, spring seats or bolsters from dropping to track structure in event of a hanger or spring failure...

49 CFR 229.65 - Spring rigging.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Spring rigging. 229.65 Section 229.65....65 Spring rigging. (a) Protective construction or safety hangers shall be provided to prevent spring planks, spring seats or bolsters from dropping to track structure in event of a hanger or spring failure...

49 CFR 229.65 - Spring rigging.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Spring rigging. 229.65 Section 229.65....65 Spring rigging. (a) Protective construction or safety hangers shall be provided to prevent spring planks, spring seats or bolsters from dropping to track structure in event of a hanger or spring failure...

49 CFR 229.65 - Spring rigging.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Spring rigging. 229.65 Section 229.65....65 Spring rigging. (a) Protective construction or safety hangers shall be provided to prevent spring planks, spring seats or bolsters from dropping to track structure in event of a hanger or spring failure...

49 CFR 229.65 - Spring rigging.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Spring rigging. 229.65 Section 229.65....65 Spring rigging. (a) Protective construction or safety hangers shall be provided to prevent spring planks, spring seats or bolsters from dropping to track structure in event of a hanger or spring failure...

16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070 ...

16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070 ...

Injecting rabies immunoglobulin (RIG) into wounds only: A significant saving of lives and costly RIG.

PubMed

Bharti, Omesh Kumar; Madhusudana, Shampur Narayan; Wilde, Henry

2017-04-03

An increasing number of dog bite victims were being presented to public hospitals in Himachal Pradesh in 2014 amidst virtual non availability of any rabies immunoglobulin (RIG). Only a small quantity of equine rabies immunoglobulin (eRIG) was available from the government owned Central Research Institute (CRI) Kasauli. This available eRIG was used in 269 patients as an emergency response and only for local infiltration of severe bite wounds by suspected rabid dogs. This was followed by rabies vaccination, using the WHO approved intra-dermal Thai Red Cross Society vaccination schedule. A subgroup of 26 patients were later identified who had been severely bitten by laboratory confirmed rabid dogs. They were followed for more than one year and all were found to be alive.

Feasibility Assessment of Thermal Barrier Seals for Extreme Transient Temperatures

NASA Technical Reports Server (NTRS)

Steinetz, Bruce M.; Dunlap, Patrick H., Jr.

1998-01-01

The assembly joints of modem solid rocket motor cases are generally sealed using conventional O-ring type seals. The 5500+ F combustion gases produced by rocket motors are kept a safe distance away from the seals by thick layers of phenolic insulation. Special compounds are used to fill insulation gaps leading up to the seals to prevent a direct flowpath to them. Design criteria require that the seals should not experience torching or charring during operation, or their sealing ability would be compromised. On limited occasions, NASA has observed charring of the primary O-rings of the Space Shuttle solid rocket nozzle assembly joints due to parasitic leakage paths opening up in the gap-fill compounds during rocket operation. NASA is investigating different approaches for preventing torching or charring of the primary O-rings. One approach is to implement a braided rope seal upstream of the primary O-ring to serve as a thermal barrier that prevents the hot gases from impinging on the O-ring seals. This paper presents flow, resiliency, and thermal resistance for several types of NASA rope seals braided out of carbon fibers. Burn tests were performed to determine the time to burn through each of the seals when exposed to the flame of an oxyacetylene torch (5500 F), representative of the 5500 F solid rocket motor combustion temperatures. Rope seals braided out of carbon fibers endured the flame for over six minutes, three times longer than solid rocket motor burn time. Room and high temperature flow tests are presented for the carbon seals for different amounts of linear compression. Room temperature compression tests were performed to assess seal resiliency and unit preloads as a function of compression. The thermal barrier seal was tested in a subscale "char" motor test in which the seal sealed an intentional defect in the gap insulation. Temperature measurements indicated that the seal blocked 2500 F combustion gases on the upstream side with very little temperature rise on the downstream side.

Materials and Procedures for Sealing and Filling Cracks in Asphalt-Surfaced Pavements : Manual of Practice (Update)

DOT National Transportation Integrated Search

1999-06-01

The Strategic Highway Research Program (SHRP) H-106 maintenance experiment and the Federal Highway Administration (FHWA) Long-Term Monitoring (LTM) of Pavement Maintenance Materials Test Sites project studied the treatment (sealing and filling) of cr...

77 FR 31423 - Qualification of Drivers; Application for Exemptions; National Association of the Deaf

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-25

... dump trucks and tractor trailer trucks. He would like to drive tractor trailer trucks in interstate... CDL and drive heavy equipment such as a dump truck, or rig truck with a trailer, if granted an... intrastate commerce. This includes experience driving heavy equipment, dump trucks and public works trucks...

Intrusion of Soil Water through Pipe Cracks

EPA Science Inventory

This report describes a series of experiments conducted at U.S. EPA’s Test and Evaluation Facility in 2013-2014 to study the intrusion of contaminated soil water into a pipe crack during simulated backflow events. A test rig was used consisting of a 3’ x 3’ x 3’ acrylic soil bo...

Rigging the Deck: Selecting Good Problems for Expert-Novice Card-Sorting Experiments

ERIC Educational Resources Information Center

Wolf, Steven F.; Dougherty, Daniel P.; Kortemeyer, Gerd

2012-01-01

A seminal study by Chi "et al." firmly established the paradigm that novices categorize physics problems by "surface features" (e.g., "incline," "pendulum," "projectile motion," etc.), while experts use "deep structure" (e.g., "energy conservation," "Newton 2," etc.). Yet, efforts to replicate the study frequently fail, since the ability to…

A Pilot-Scale Heat Recovery System for Computer Process Control Teaching and Research.

ERIC Educational Resources Information Center

Callaghan, P. J.; And Others

1988-01-01

Describes the experimental system and equipment including an interface box for displaying variables. Discusses features which make the circuit suitable for teaching and research in computing. Feedforward, decoupling, and adaptive control, examination of digital filtering, and a cascade loop are teaching experiments utilizing this rig. Diagrams and…

Vibration modelling and verifications for whole aero-engine

NASA Astrophysics Data System (ADS)

Chen, G.

2015-08-01

In this study, a new rotor-ball-bearing-casing coupling dynamic model for a practical aero-engine is established. In the coupling system, the rotor and casing systems are modelled using the finite element method, support systems are modelled as lumped parameter models, nonlinear factors of ball bearings and faults are included, and four types of supports and connection models are defined to model the complex rotor-support-casing coupling system of the aero-engine. A new numerical integral method that combines the Newmark-β method and the improved Newmark-β method (Zhai method) is used to obtain the system responses. Finally, the new model is verified in three ways: (1) modal experiment based on rotor-ball bearing rig, (2) modal experiment based on rotor-ball-bearing-casing rig, and (3) fault simulations for a certain type of missile turbofan aero-engine vibration. The results show that the proposed model can not only simulate the natural vibration characteristics of the whole aero-engine but also effectively perform nonlinear dynamic simulations of a whole aero-engine with faults.

Experiments with a New, Unique Large-Scale Rig Investigating the Effects of Background System Rotation on Vortex Rings in Water

NASA Astrophysics Data System (ADS)

Brend, Mark A.; Verzicco, Roberto

2005-11-01

We introduce our unique, new large-scale experimental facility [1] designed for our long-term research program investigating the effects of background system rotation on the stability and the dynamics of vortex rings. The new rig constitutes a large water-filled tank positioned on a rotating turntable and its overall height and diameter are 5.7m and 1.4 m, respectively. First experimental and computational results of our program are summarized. We will show various videos of flow visualizations that illustrate some major, qualitative differences between rings propagating in rotating and non-rotating flows. Some of the investigated characteristics of the vortex rings include their translation velocity, the velocity field inside and surrounding the rings, and, in particular, their stability. We will briefly outline experiments employing the relatively new Ultrasonic-Velocity-Profiler technique (UVP). This technique appears to be particularly suited for some of our measurements and it was, as far as we are aware, not previously used in the context of vortex-ring studies. [1] http://www.eng.warwick.ac.uk/staff/pjt/turntabpics/voriskt.html

Characterization and durability testing of plasma-sprayed zirconia-yttria and hafnia-yttria thermal barrier coatings. Part 2: Effect of spray parameters on the performance of several hafnia-yttria and zirconia-yttria coatings

NASA Technical Reports Server (NTRS)

Miller, Robert A.; Leissler, George W.

1993-01-01

This is the second of two reports which discuss initial experiments on thermal barrier coatings prepared and tested in newly upgraded plasma spray and burner rig test facilities at LeRC. The first report, part 1, describes experiments designed to establish the spray parameters for the baseline zirconia-yttria coating. Coating quality was judged primarily by the response to burner rig exposure, together with a variety of other characterization approaches including thermal diffusivity measurements. That portion of the study showed that the performance of the baseline NASA coating was not strongly sensitive to processing parameters. In this second part of the study, new hafnia-yttria coatings were evaluated with respect to both baseline and alternate zirconia-yttria coatings. The hafnia-yttria and the alternate zirconia-yttria coatings were very sensitive to plasma-spray parameters in that high-quality coatings were obtained only when specific parameters were used. The reasons for this important observation are not understood.

Citizen science data suggest that a novel rig improves landing rate and reduces injury and handling time in recreational angling with artificial lures in Baltic pike (Esox lucius).

PubMed

Bursell, Jens Jakob; Arlinghaus, Robert

2018-01-01

The optimal terminal gear in hook-and-line recreational fishing maximizes landing rates and minimizes injury to the fish because some fish will be released after capture. We designed a novel rig configuration in artificial lure fishing for top predators and examined its effectiveness in angling for Baltic northern pike ( Esox lucius ) using a citizen science approach based on observational data collected from volunteer anglers in the field. The novel rig included two changes to traditional rig designs common to artificial lure angling. First, hooks were mounted in a way giving better hook exposure and eliminating lever-arm effects from the lure to the hooks once a fish is hooked. This construction allowed the second change, being a shift to hooks 4-5 sizes smaller than those used on traditional hook mounts. We analysed observational data collected by volunteer anglers using either the novel rig or a standard rig mount in two types of artificial lures (softbait and hardbait) of the same size (about 17 cm). Using N  = 768 pike contacts as input data, we showed the landing rates of pike targeted with artificial lures significantly and substantially increased from 45% with normal-rigs to 85% when the same lure types were fished with the novel rig configuration. Lure type and water temperature had no effects on landing rates. Moreover, hardbaits on normal-rigs produced significantly more injury, bleeding and elevated unhooking time compared to fish captured on hardbaits with release-rigs. We conclude that simple changes to traditional hook sizes and mounts in lure fishing may benefit both anglers and the fishes that are to be released and that citizen science projects with volunteer anglers are able to provide good data in proof-of-concept studies. Further experimental studies are needed to differentiate hook size from hook mount effects because both variables were confounded in the results of the observational data presented here.

Non-contacting gas lubricated face seals for high p x v-values

NASA Technical Reports Server (NTRS)

Glienicke, J.; Launert, A.; Schums, H.; Kohring, B.

1994-01-01

The authors discuss recently developed mathematical fundamentals concerning the calculation of noncontacting gas lubricated face seals. They carried out extensive experiments using three different designs at pressures up to 10 MPa and sliding velocities up to 110 m/s. A comparison between the experimental results and the calculations indicates that a stable operation without wear can be ensured in all cases, provided that the materials and geometrical parameters of the seal have been properly chosen.

The destructive impact of the rogue waves

NASA Astrophysics Data System (ADS)

Shamin, Roman

2013-04-01

In our talk rogue waves at the ocean will be considered. By means of numerical modeling dangerous impact of rogue waves on the ships and oil rigs is calculated. Cases when these waves can bring in accident are considered. Using statistics of emergence of waves (see [1]-[2]), it is possible to estimate risks in each case. These results can be used for safety of the ships and oil rigs from rogue waves. References [1] V.E. Zakharov, A.I. Dyachenko, R.V. Shamin. How probability for freak wave formation can be found // THE EUROPEAN PHYSICAL JOURNAL - SPECIAL TOPICS Volume 185, Number 1, 113-124, DOI: 10.1140/epjst/e2010-01242-y [2] V.E. Zakharov, R.V. Shamin. Statistics of rogue waves in computer experiments // JETP Letters, 2012, V. 96, Issue 1, pp 66-69.

Rotating Rig Development for Droplet Deformation/Breakup and Impact Induced by Aerodynamic Surfaces

NASA Technical Reports Server (NTRS)

Feo, A.; Vargas, M.; Sor, A.

2012-01-01

This work presents the development of a Rotating Rig Facility by the Instituto Nacional de Tecnica Aeroespacial (INTA) in cooperation with the NASA Glenn Research Center. The facility is located at the INTA installations near Madrid, Spain. It has been designed to study the deformation, breakup and impact of large droplets induced by aerodynamic bodies. The importance of these physical phenomena is related to the effects of Supercooled Large Droplets in icing clouds on the impinging efficiency of the droplets on the body, that may change should these phenomena not be taken into account. The important variables and the similarity parameters that enter in this problem are presented. The facility's components are described and some possible set-ups are explained. Application examples from past experiments are presented in order to indicate the capabilities of the new facility.

Design and commission of an experimental test rig to apply a full-scale pressure load on composite sandwich panels representative of an aircraft secondary structure

NASA Astrophysics Data System (ADS)

Crump, D. A.; Dulieu-Barton, J. M.; Savage, J.

2010-01-01

This paper describes the design of a test rig, which is used to apply a representative pressure load to a full-scale composite sandwich secondary aircraft structure. A generic panel was designed with features to represent those in the composite sandwich secondary aircraft structure. To provide full-field strain data from the panels, the test rig was designed for use with optical measurement techniques such as thermoelastic stress analysis (TSA) and digital image correlation (DIC). TSA requires a cyclic load to be applied to a structure for the measurement of the strain state; therefore, the test rig has been designed to be mounted on a standard servo-hydraulic test machine. As both TSA and DIC require an uninterrupted view of the surface of the test panel, an important consideration in the design is facilitating the optical access for the two techniques. To aid the test rig design a finite element (FE) model was produced. The model provides information on the deflections that must be accommodated by the test rig, and ensures that the stress and strain levels developed in the panel when loaded in the test rig would be sufficient for measurement using TSA and DIC. Finally, initial tests using the test rig have shown it to be capable of achieving the required pressure and maintaining a cyclic load. It was also demonstrated that both TSA and DIC data can be collected from the panels under load, which are used to validate the stress and deflection derived from the FE model.

Proapoptotic signaling induced by RIG-I and MDA-5 results in type I interferon–independent apoptosis in human melanoma cells

PubMed Central

Besch, Robert; Poeck, Hendrik; Hohenauer, Tobias; Senft, Daniela; Häcker, Georg; Berking, Carola; Hornung, Veit; Endres, Stefan; Ruzicka, Thomas; Rothenfusser, Simon; Hartmann, Gunther

2009-01-01

The retinoic acid–inducible gene I (RIG-I) and melanoma differentiation–associated antigen 5 (MDA-5) helicases sense viral RNA in infected cells and initiate antiviral responses such as the production of type I IFNs. Here we have shown that RIG-I and MDA-5 also initiate a proapoptotic signaling pathway that is independent of type I IFNs. In human melanoma cells, this signaling pathway required the mitochondrial adapter Cardif (also known as IPS-1) and induced the proapoptotic BH3-only proteins Puma and Noxa. RIG-I– and MDA-5–initiated apoptosis required Noxa but was independent of the tumor suppressor p53. Triggering this pathway led to efficient activation of mitochondrial apoptosis, requiring caspase-9 and Apaf-1. Surprisingly, this proapoptotic signaling pathway was also active in nonmalignant cells, but these cells were much less sensitive to apoptosis than melanoma cells. Endogenous Bcl-xL rescued nonmalignant, but not melanoma, cells from RIG-I– and MDA-5–mediated apoptosis. In addition, we confirmed the results of the in vitro studies, demonstrating that RIG-I and MDA-5 ligands both reduced human tumor lung metastasis in immunodeficient NOD/SCID mice. These results identify an IFN-independent antiviral signaling pathway initiated by RIG-I and MDA-5 that activates proapoptotic signaling and, unless blocked by Bcl-xL, results in apoptosis. Due to their immunostimulatory and proapoptotic activity, RIG-I and MDA-5 ligands have therapeutic potential due to their ability to overcome the characteristic resistance of melanoma cells to apoptosis. PMID:19620789

Estimating gas kick arrival to better manage surface flow

DOE Office of Scientific and Technical Information (OSTI.GOV)

Merryman, J.

1997-06-01

There are many quality, expertly designed programs for teaching rig crews prevention and control of well kicks. Much time and money has been spent training rig crews for procedures and methods to avoid kicks, and what to do if you have one, even though all accepted practices were followed. One false/bad result from this training is that individuals have come away from this training believing that once a gas kick is circulated through the choke, all problems are simple and manageable, i.e., the danger has passed. What you do with gas once it is downstream of the choke has notmore » been given enough emphasis in some training programs, including the one the author is involved in. This article, prepared from experiences on actual rig locations in Wyoming and Argentina, and developed for well control training in Parker Drilling Co.`s Training Center, Odessa, Texas, describes a method for estimating the time, in pump strokes and/or minutes, for a gas kick to reach surface. Reducing pump strokes (circulating flowrate) and knowing available time to gas kick arrival allows the crew to prepare surface handling equipment. Common misconceptions about gas handling and potential resulting problems are noted. An example calculation describes the calculation procedure.« less

29 CFR 1926.753 - Hoisting and rigging.

Code of Federal Regulations, 2010 CFR

2010-07-01

... lift rigging procedure. (1) A multiple lift shall only be performed if the following criteria are met: (i) A multiple lift rigging assembly is used; (ii) A maximum of five members are hoisted per lift... multiple lift have been trained in these procedures in accordance with § 1926.761(c)(1). (v) No crane is...

Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway.

PubMed

Sanchez, Jacint G; Chiang, Jessica J; Sparrer, Konstantin M J; Alam, Steven L; Chi, Michael; Roganowicz, Marcin D; Sankaran, Banumathi; Gack, Michaela U; Pornillos, Owen

2016-08-02

Antiviral response pathways induce interferon by higher-order assembly of signaling complexes called signalosomes. Assembly of the RIG-I signalosome is regulated by K63-linked polyubiquitin chains, which are synthesized by the E3 ubiquitin ligase, TRIM25. We have previously shown that the TRIM25 coiled-coil domain is a stable, antiparallel dimer that positions two catalytic RING domains on opposite ends of an elongated rod. We now show that the RING domain is a separate self-association motif that engages ubiquitin-conjugated E2 enzymes as a dimer. RING dimerization is required for catalysis, TRIM25-mediated RIG-I ubiquitination, interferon induction, and antiviral activity. We also provide evidence that RING dimerization and E3 ligase activity are promoted by binding of the TRIM25 SPRY domain to the RIG-I effector domain. These results indicate that TRIM25 actively participates in higher-order assembly of the RIG-I signalosome and helps to fine-tune the efficiency of the RIG-I-mediated antiviral response. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Comparative analysis of viral RNA signatures on different RIG-I-like receptors

PubMed Central

Sanchez David, Raul Y; Combredet, Chantal; Sismeiro, Odile; Dillies, Marie-Agnès; Jagla, Bernd; Coppée, Jean-Yves; Mura, Marie; Guerbois Galla, Mathilde; Despres, Philippe; Tangy, Frédéric; Komarova, Anastassia V

2016-01-01

The RIG-I-like receptors (RLRs) play a major role in sensing RNA virus infection to initiate and modulate antiviral immunity. They interact with particular viral RNAs, most of them being still unknown. To decipher the viral RNA signature on RLRs during viral infection, we tagged RLRs (RIG-I, MDA5, LGP2) and applied tagged protein affinity purification followed by next-generation sequencing (NGS) of associated RNA molecules. Two viruses with negative- and positive-sense RNA genome were used: measles (MV) and chikungunya (CHIKV). NGS analysis revealed that distinct regions of MV genome were specifically recognized by distinct RLRs: RIG-I recognized defective interfering genomes, whereas MDA5 and LGP2 specifically bound MV nucleoprotein-coding region. During CHIKV infection, RIG-I associated specifically to the 3’ untranslated region of viral genome. This study provides the first comparative view of the viral RNA ligands for RIG-I, MDA5 and LGP2 in the presence of infection. DOI: http://dx.doi.org/10.7554/eLife.11275.001 PMID:27011352

Truncation of the C-terminal region of Toscana Virus NSs protein is critical for interferon-β antagonism and protein stability.

PubMed

Gori Savellini, Gianni; Gandolfo, Claudia; Cusi, Maria Grazia

2015-12-01

Toscana Virus (TOSV) is a Phlebovirus responsible for central nervous system (CNS) injury in humans. The TOSV non-structural protein (NSs), which interacting with RIG-I leads to its degradation, was analysed in the C terminus fragment in order to identify its functional domains. To this aim, two C-terminal truncated NSs proteins, Δ1C-NSs (aa 1-284) and Δ2C-NSs (aa 1-287) were tested. Only Δ1C-NSs did not present any inhibitory effect on RIG-I and it showed a greater stability than the whole NSs protein. Moreover, the deletion of the TLQ aa sequence interposed between the two ΔC constructs caused a greater accumulation of the protein with a weak inhibitory effect on RIG-I, indicating some involvement of these amino acids in the NSs activity. Nevertheless, all the truncated proteins were still able to interact with RIG-I, suggesting that the domains responsible for RIG-I signaling and RIG-I interaction are mapped on different regions of the protein. Copyright © 2015 Elsevier Inc. All rights reserved.

Dynamic Spin Rig Upgraded With a Five- Axis-Controlled Three-Magnetic-Bearing Support System With Forward Excitation

NASA Technical Reports Server (NTRS)

Morrison, Carlos R.; Mehmed, Oral

2003-01-01

The NASA Glenn Research Center Dynamic Spin Rig is used for experimental evaluation of vibration analysis methods and dynamic characteristics for rotating systems. Measurements are made while rotors are spun and vibrated in a vacuum chamber. The rig has been upgraded with a new active magnetic bearing rotor support and excitation system. This design is expected to provide operational improvements over the existing rig. The rig will be able to be operated in either the old or new configuration. In the old configuration, two ball bearings support the vertical shaft of the rig, with the test article located between the bearings. Because the bearings operate in a vacuum, lubrication is limited to grease. This limits bearing life and speed. In addition, the old configuration employs two voice-coil electromagnetic shakers to apply oscillatory axial forces or transverse moments to the rotor shaft through a thrust bearing. The excitation amplitudes that can be imparted to the test article with this system are not adequate for components that are highly damped. It is expected that the new design will overcome these limitations.

Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway

DOE PAGES

Sanchez, Jacint G.; Chiang, Jessica J.; Sparrer, Konstantin M. J.; ...

2016-07-14

Antiviral response pathways induce interferon by higher-order assembly of signaling complexes called signalosomes. Assembly of the RIG-I signalosome is regulated by K63-linked polyubiquitin chains, which are synthesized by the E3 ubiquitin ligase, TRIM25. We have previously shown that the TRIM25 coiled-coil domain is a stable, antiparallel dimer that positions two catalytic RING domains on opposite ends of an elongated rod. We now show that the RING domain is a separate self-association motif that engages ubiquitin-conjugated E2 enzymes as a dimer. RING dimerization is required for catalysis, TRIM25-mediated RIG-I ubiquitination, interferon induction, and antiviral activity. We also provide evidence that RINGmore » dimerization and E3 ligase activity are promoted by binding of the TRIM25 SPRY domain to the RIG-I effector domain. These results indicate that TRIM25 actively participates in higher-order assembly of the RIG-I signalosome and helps to fine-tune the efficiency of the RIG-I-mediated antiviral response.« less

Kinematic perturbation in the flexion-extension axis for two lumbar rigs during a high impact jump task.

PubMed

Portus, Marc R; Lloyd, David G; Elliott, Bruce C; Trama, Neil L

2011-05-01

The measurement of lumbar spine motion is an important step for injury prevention research during complex and high impact activities, such as cricket fast bowling or javelin throwing. This study examined the performance of two designs of a lumbar rig, previously used in gait research, during a controlled high impact bench jump task. An 8-camera retro-reflective motion analysis system was used to track the lumbar rig. Eleven athletes completed the task wearing the two different lumbar rig designs. Flexion extension data were analyzed using a fast Fourier transformation to assess the signal power of these data during the impact phase of the jump. The lumbar rig featuring an increased and pliable base of support recorded moderately less signal power through the 0-60 Hz spectrum, with statistically less magnitudes at the 0-5 Hz (p = .039), 5-10 Hz (p = .005) and 10-20 Hz (p = .006) frequency bins. A lumbar rig of this design would seem likely to provide less noisy lumbar motion data during high impact tasks.

Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome.

PubMed

Luthra, Priya; Ramanan, Parameshwaran; Mire, Chad E; Weisend, Carla; Tsuda, Yoshimi; Yen, Benjamin; Liu, Gai; Leung, Daisy W; Geisbert, Thomas W; Ebihara, Hideki; Amarasinghe, Gaya K; Basler, Christopher F

2013-07-17

The cytoplasmic pattern recognition receptor RIG-I is activated by viral RNA and induces type I IFN responses to control viral replication. The cellular dsRNA binding protein PACT can also activate RIG-I. To counteract innate antiviral responses, some viruses, including Ebola virus (EBOV), encode proteins that antagonize RIG-I signaling. Here, we show that EBOV VP35 inhibits PACT-induced RIG-I ATPase activity in a dose-dependent manner. The interaction of PACT with RIG-I is disrupted by wild-type VP35, but not by VP35 mutants that are unable to bind PACT. In addition, PACT-VP35 interaction impairs the association between VP35 and the viral polymerase, thereby diminishing viral RNA synthesis and modulating EBOV replication. PACT-deficient cells are defective in IFN induction and are insensitive to VP35 function. These data support a model in which the VP35-PACT interaction is mutually antagonistic and plays a fundamental role in determining the outcome of EBOV infection. Copyright © 2013 Elsevier Inc. All rights reserved.

Modeling Heat Transfer and Pressurization of Polymeric Methylene Diisocyanate (PMDI) Polyurethane Foam in a Sealed Container.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Scott, Sarah Nicole

Polymer foam encapsulants provide mechanical, electrical, and thermal isolation in engineered systems. It can be advantageous to surround objects of interest, such as electronics, with foams in a hermetically sealed container to protect the electronics from hostile en vironments, such as a crash that produces a fire. However, i n fire environments, gas pressure from thermal decomposition of foams can cause mechanical failure of the sealed system . In this work, a detailed study of thermally decomposing polymeric methylene diisocyanate (PMDI) - polyether - polyol based polyurethane foam in a sealed container is presented . Both experimental and computational workmore » is discussed. Three models of increasing physics fidelity are presented: No Flow, Porous Media, and Porous Media with VLE. Each model us described in detail, compared to experiment , and uncertainty quantification is performed. While the Porous Media with VLE model matches has the best agreement with experiment, it also requires the most computational resources.« less

HIFiRE Direct-Connect Rig (HDCR) Phase I Ground Test Results from the NASA Langley Arc-Heated Scramjet Test Facility

NASA Technical Reports Server (NTRS)

Hass, Neal E.; Cabell, Karen F.; Storch, Andrea M.

2010-01-01

The initial phase of hydrocarbon-fueled ground tests supporting Flight 2 of the Hypersonic International Flight Research Experiment (HIFiRE) Program has been conducted in the NASA Langley Arc-Heated Scramjet Test Facility (AHSTF). The HIFiRE Program, an Air Force-lead international cooperative program includes eight different flight test experiments designed to target specific challenges of hypersonic flight. The second of the eight planned flight experiments is a hydrocarbon-fueled scramjet flight test intended to demonstrate dual-mode to scramjet-mode operation and verify the scramjet performance prediction and design tools. A performance goal is the achievement of a combusted fuel equivalence ratio greater than 0.7 while in scramjet mode. The ground test rig, designated the HIFiRE Direct Connect Rig (HDCR), is a full-scale, heat sink, direct-connect ground test article that duplicates both the flowpath lines and the instrumentation layout of the isolator and combustor portion of the flight test hardware. The primary objectives of the HDCR Phase I tests are to verify the operability of the HIFiRE isolator/combustor across the Mach 6.0-8.0 flight regime and to establish a fuel distribution schedule to ensure a successful mode transition prior to the HiFIRE payload Critical Design Review. Although the phase I test plans include testing over the Mach 6 to 8 flight simulation range, only Mach 6 testing will be reported in this paper. Experimental results presented here include flowpath surface pressure, temperature, and heat flux distributions that demonstrate the operation of the flowpath over a small range of test conditions around the nominal Mach 6 simulation, as well as a range of fuel equivalence ratios and fuel injection distributions. Both ethylene and a mixture of ethylene and methane (planned for flight) were tested. Maximum back pressure and flameholding limits, as well as a baseline fuel schedule, that covers the Mach 5.84-6.5 test space have been identified.

Emerging Role of Ubiquitination in Antiviral RIG-I Signaling

PubMed Central

Maelfait, Jonathan

2012-01-01

Summary: Detection of viruses by the innate immune system involves the action of specialized pattern recognition receptors. Intracellular RIG-I receptors sense the presence of viral nucleic acids in infected cells and trigger signaling pathways that lead to the production of proinflammatory and antiviral proteins. Over the past few years, posttranslational modification of RIG-I and downstream signaling proteins by different types of ubiquitination has been found to be a key event in the regulation of RIG-I-induced NF-κB and interferon regulatory factor 3 (IRF3) activation. Multiple ubiquitin ligases, deubiquitinases, and ubiquitin binding scaffold proteins contribute to both positive and negative regulation of the RIG-I-induced antiviral immune response. A better understanding of the function and activity of these proteins might eventually lead to the development of novel therapeutic approaches for management of viral diseases. PMID:22390971

Analog Video Authentication and Seal Verification Equipment Development

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gregory Lancaster

Under contract to the US Department of Energy in support of arms control treaty verification activities, the Savannah River National Laboratory in conjunction with the Pacific Northwest National Laboratory, the Idaho National Laboratory and Milagro Consulting, LLC developed equipment for use within a chain of custody regime. This paper discussed two specific devices, the Authentication Through the Lens (ATL) analog video authentication system and a photographic multi-seal reader. Both of these devices have been demonstrated in a field trial, and the experience gained throughout will also be discussed. Typically, cryptographic methods are used to prove the authenticity of digital imagesmore » and video used in arms control chain of custody applications. However, in some applications analog cameras are used. Since cryptographic authentication methods will not work on analog video streams, a simple method of authenticating analog video was developed and tested. A photographic multi-seal reader was developed to image different types of visual unique identifiers for use in chain of custody and authentication activities. This seal reader is unique in its ability to image various types of seals including the Cobra Seal, Reflective Particle Tags, and adhesive seals. Flicker comparison is used to compare before and after images collected with the seal reader in order to detect tampering and verify the integrity of the seal.« less

Belukha whale (delphinapterus leucas) responses to industrial noise in Nushagak Bay, Alaska: 1983

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stewart, B.S.; Awbrey, F.T.; Evans, W.E.

1983-01-01

Between 15 June and 14 July 1983 the authors conducted playback experiments with belukha whales in the Snake River, Alaska, using sounds recorded near an operating oil-drilling rig. The objectives of these experiments were to quantify behavioral responses of belukha whales to oil drilling noise in an area where foreign acoustic stimuli were absent, and to test the hypothesis that beluhka whales would not approach a source of loud sound.

The role of glucocorticoids in naturally fasting grey seal (Halichoerus grypus) pups: dexamethasone stimulates mass loss and protein utilisation, but not departure from the colony.

PubMed

Bennett, K A; Fedak, M A; Moss, S E W; Pomeroy, P P; Speakman, J R; Hall, A J

2013-03-15

Seals must manage their energy reserves carefully while they fast on land to ensure that they go to sea with sufficient fuel to sustain them until they find food. Glucocorticoids (GCs) have been implicated in the control of fuel metabolism and termination of fasting in pinnipeds. Here we tested the hypothesis that dexamethasone, an artificial GC, increases fat and protein catabolism, and induces departure from the breeding colony in wild, fasting grey seal pups. A single intramuscular dose of dexamethasone completely suppressed cortisol production for 24-72 h, demonstrating activation of GC receptors. In experiment 1, we compared the effects of a single dose of dexamethasone or saline administered 10 days after weaning on fasting mass and body composition changes, cortisol, blood urea nitrogen (BUN) and glucose levels, and timing of departure from the colony. In experiment 2, we investigated the effects of dexamethasone on short-term (5 days) changes in mass loss, body composition and BUN levels. In experiment 1, dexamethasone induced a short-lived increase in mass loss, but there was no difference in timing of departure between dexamethasone- and saline-treated pups (N=10). In experiment 2, dexamethasone increased protein and water loss and prevented a decrease in BUN levels (N=11). Our data suggest changes in cortisol contribute to regulation of protein catabolism in fasting seal pups, irrespective of the sex of the animal, but do not terminate fasting. By affecting the rate of protein depletion, lasting changes in cortisol levels could influence the amount of time seal pups have to find food, and thus may have important consequences for their survival.

Molecular characterisation of RIG-I-like helicases in the black flying fox, Pteropus alecto.

PubMed

Cowled, Christopher; Baker, Michelle L; Zhou, Peng; Tachedjian, Mary; Wang, Lin-Fa

2012-04-01

The RIG-I like helicases, RIG-I, mda5 and LGP2 are an evolutionarily conserved family of cytosolic pattern recognition receptors important in the recognition of viral RNA, and responsible for the innate induction of interferons and proinflammatory cytokines upon viral infection. Bats are natural reservoir hosts to a variety of RNA viruses that cause significant morbidity and mortality in other species; however the mechanisms responsible for the control of viral replication in bats are not understood. This report describes the molecular cloning and expression analysis of RIG-I, mda5 and LGP2 genes in the fruit bat Pteropus alecto, and is the first description of RIG-I like helicases from any species of bat. Our results demonstrate that P. alecto RIG-I, mda5 and LGP2 have similar primary structures and tissue expression patterns to their counterparts in humans and other mammals. Stimulation of bat kidney cells with synthetic dsRNA (poly I:C) induced high levels of interferon β and rapid upregulation of all three helicases. These findings reveal that the cytoplasmic virus sensing machinery is present and intact in P. alecto. This study provides the foundation for further investigations into the interactions between bat RIG-I-like helicases and viruses to elucidate the mechanisms responsible for the asymptomatic nature of viral infections in bats. Crown Copyright © 2011. Published by Elsevier Ltd. All rights reserved.

Compressor discharge film riding face seals

NASA Technical Reports Server (NTRS)

Munson, John

1994-01-01

Seals examined were the eight-pad Rayleigh step, the tapered spiral groove, and two hydrostatic seals. The spiral groove configuration is the preferred choice because of superior stiffness. Second choice is Rayleigh step because of combined higher operating film thickness and good stiffness at low clearance. Recess hydrostatic has reasonable performance, but stiffness falls off at low clearance. Also, pneumatic hammer characteristics must be investigated. Experience at high pressure ratios is limited. An advantage is that it would have good low speed performance.

Increasing Geothermal Energy Demand: The Need for Urbanization of the Drilling Industry

ERIC Educational Resources Information Center

Teodoriu, Catalin; Falcone, Gioia

2008-01-01

Drilling wells in urban spaces requires special types of rigs that do not conflict with the surrounding environment. For this, a mutation of the current drilling equipment is necessary into what can be defined as an "urbanized drilling rig." Noise reduction, small footprint, and "good looking" rigs all help persuade the general public to accept…

Mechanical and thermal characterization of a ceramic/glass composite seal for solid oxide fuel cells

NASA Astrophysics Data System (ADS)

Dev, Bodhayan; Walter, Mark E.; Arkenberg, Gene B.; Swartz, Scott L.

2014-01-01

Solid oxide fuel cells (SOFCs) require seals that can function in harsh, elevated temperature environments. Comprehensive characterization and understanding of seals is needed for commercially viable SOFCs. The present research focuses on a novel ceramic/glass composite seal that is produced by roller compaction or tape casting of glass and ceramic powders and an organic binder. Upon heat treatment, micro-voids and surface anomalies are formed. Increased heating and cooling rates during the heat treatment resulted in more and larger voids. The first goal of the current research is to suggest an appropriate heating and cooling rate to minimize the formation of microstructural defects. After identifying an appropriate cure cycle, seals were thermally cycled and then characterized with laser dilatometry, X-ray diffraction, and sonic resonance. From these experiments the crystalline phases, thermal expansion, and elastic properties were determined. Subsequently compression testing with an acoustic emission (AE) sensor and post-test microstructural analysis were used to identify the formation of damage. By fully understanding the characteristics of this ceramic/glass composite seal, next generation seals can be fabricated for improved performance.

Effect of cultural conditions on the seed-to-seed growth of Arabidopsis and Cardamine - A study of growth rates and reproductive development as affected by test tube seals

NASA Technical Reports Server (NTRS)

Hoshizaki, T.

1982-01-01

The effects of test tube seals on the growth, flowering, and seed pod formation of Arabidopsis thaliana (L.) Heynh., mouse ear cress, and Cardamine oligosperma Nutt, bitter cress, are studied in order to assess the conditions used in weightlessness experiments. Among other results, it is found that the growth (height) and flowering (date of bud appearance) were suppressed in mouse ear cress in tubes sealed with Saran. Seed pod formation which occurred by day 45 in open-to-air controls, was still lacking in the sealed plants even up to day 124. The growth and flowering of bitter cress were also suppressed by the Saran seal, although up to day 55 the Saran-sealed plants were taller. It is suggested that atmospheric composition was the cause of the suppression of growth, flowering, and seed pod development in these plants, since the mouse ear cress renewed their growth and then set seed pods after the Saran seal was ruptured.

HIFiRE Direct-Connect Rig (HDCR) Phase I Scramjet Test Results from the NASA Langley Arc-Heated Scramjet Test Facility

NASA Technical Reports Server (NTRS)

Cabell, Karen; Hass, Neal; Storch, Andrea; Gruber, Mark

2011-01-01

A series of hydrocarbon-fueled direct-connect scramjet ground tests has been completed in the NASA Langley Arc-Heated Scramjet Test Facility (AHSTF) at simulated Mach 8 flight conditions. These experiments were part of an initial test phase to support Flight 2 of the Hypersonic International Flight Research Experimentation (HIFiRE) Program. In this flight experiment, a hydrocarbon-fueled scramjet is intended to demonstrate transition from dual-mode to scramjet-mode operation and verify the scramjet performance prediction and design tools A performance goal is the achievement of a combusted fuel equivalence ratio greater than 0.7 while in scramjet mode. The ground test rig, designated the HIFiRE Direct Connect Rig (HDCR), is a full-scale, heat sink test article that duplicates both the flowpath lines and a majority of the instrumentation layout of the isolator and combustor portion of the flight test hardware. The primary objectives of the HDCR Phase I tests were to verify the operability of the HIFiRE isolator/combustor across the simulated Mach 6-8 flight regime and to establish a fuel distribution schedule to ensure a successful mode transition. Both of these objectives were achieved prior to the HiFIRE Flight 2 payload Critical Design Review. Mach 8 ground test results are presented in this report, including flowpath surface pressure distributions that demonstrate the operation of the flowpath in scramjet-mode over a small range of test conditions around the nominal Mach 8 simulation, as well as over a range of fuel equivalence ratios. Flowpath analysis using ground test data is presented elsewhere; however, limited comparisons with analytical predictions suggest that both scramjet-mode operation and the combustion performance objective are achieved at Mach 8 conditions.

A method for testing railway wheel sets on a full-scale roller rig

NASA Astrophysics Data System (ADS)

Liu, Binbin; Bruni, Stefano

2015-09-01

Full-scale roller rigs for tests on a single axle enable the investigation of several dynamics and durability problems related with the design and operation of the railway rolling stock. In order to exploit the best potential of this test equipment, appropriate test procedures need to be defined, particularly in terms of actuators' references, to make sure that meaningful wheel -rail contact conditions can be reproduced. The aim of this paper is to propose a new methodology to define the forces to be generated by the actuators in the rig in order to best reproduce the behaviour of a wheel set and especially the wheel -rail contact forces in a running condition of interest as obtained either from multi-body system (MBS) simulation or from on-track measurements. The method is supported by the use of a mathematical model of the roller rig and uses an iterative correction scheme, comparing the time histories of the contact force components from the roller rig test as predicted by the mathematical model to a set of target contact force time histories. Two methods are introduced, the first one considering a standard arrangement of the roller rig, the second one assuming that a differential gear is introduced in the rig, allowing different rolling speeds of the two rollers. Results are presented showing that the deviation of the roller rig test results from the considered targets can be kept within low tolerances (1% approximately) as far as the vertical and lateral contact forces on both wheels are concerned. For the longitudinal forces, larger deviations are obtained except in the case where a differential gear is introduced.

Development of a low cost test rig for standalone WECS subject to electrical faults.

PubMed

Himani; Dahiya, Ratna

2016-11-01

In this paper, a contribution to the development of low-cost wind turbine (WT) test rig for stator fault diagnosis of wind turbine generator is proposed. The test rig is developed using a 2.5kW, 1750 RPM DC motor coupled to a 1.5kW, 1500 RPM self-excited induction generator interfaced with a WT mathematical model in LabVIEW. The performance of the test rig is benchmarked with already proven wind turbine test rigs. In order to detect the stator faults using non-stationary signals in self-excited induction generator, an online fault diagnostic technique of DWT-based multi-resolution analysis is proposed. It has been experimentally proven that for varying wind conditions wavelet decomposition allows good differentiation between faulty and healthy conditions leading to an effective diagnostic procedure for wind turbine condition monitoring. Copyright © 2016 ISA. Published by Elsevier Ltd. All rights reserved.

Regenerative Fuel Cell Test Rig at Glenn Research Center

NASA Technical Reports Server (NTRS)

Chang, Bei-Jiann; Johnson, Donald W.; Garcia, Christopher P.; Jakupca, Ian J.; Scullin, Vincent J.; Bents, David J.

2003-01-01

The regenerative fuel cell development effort at Glenn Research Center (GRC) involves the integration of a dedicated fuel cell and electrolyzer into an energy storage system test rig. The test rig consists of a fuel cell stack, an electrolysis stack, cooling pumps, a water transfer pump, gas recirculation pumps, phase separators, storage tanks for oxygen (O2) and hydrogen (H2), heat exchangers, isolation valves, pressure regulators, interconnecting tubing, nitrogen purge provisions, and instrumentation for control and monitoring purposes. The regenerative fuel cell (RFC) thus formed is a completely closed system which is capable of autonomous cyclic operation. The test rig provides direct current (DC) load and DC power supply to simulate power consumption and solar power input. In addition, chillers are used as the heat sink to dissipate the waste heat from the electrochemical stack operation. Various vents and nitrogen (N2) sources are included in case inert purging is necessary to safe the RFC test rig.

NASA Lewis Research Center lean-, rich-burn materials test burner rig

NASA Technical Reports Server (NTRS)

Stearns, C. A.; Robinson, R. C.

1994-01-01

The lean-, rich-burn materials test burner rig at NASA LeRC is used to evaluate the high temperature environmental durability of aerospace materials. The rig burns jet fuel and pressurized air, and sample materials can be subjected to both lean-burn and rich-burn environments. As part of NASA's Enabling Propulsion Materials (EPM) program, an existing rig was adapted to simulate the rich-burn quick-quench lean-burn (RQL) combustor concept which is being considered for the HSCT (high speed civil transport) aircraft. RQL materials requirements exceed that of current superalloys, thus ceramic matrix composites (CMC's) emerged as the leading candidate materials. The performance of these materials in the quasi reducing environment of the rich-burn section of the RQL is of fundamental importance to materials development. This rig was developed to conduct such studies, and its operation and capabilities are described.

A New High-Speed Oil-Free Turbine Engine Rotordynamic Simulator Test Rig

NASA Technical Reports Server (NTRS)

Howard, Samuel A.

2007-01-01

A new test rig has been developed for simulating high-speed turbomachinery rotor systems using Oil-Free foil air bearing technology. Foil air bearings have been used in turbomachinery, primarily air cycle machines, for the past four decades to eliminate the need for oil lubrication. The goal of applying this bearing technology to other classes of turbomachinery has prompted the fabrication of this test rig. The facility gives bearing designers the capability to test potential bearing designs with shafts that simulate the rotating components of a target machine without the high cost of building "make-and-break" hardware. The data collected from this rig can be used to make design changes to the shaft and bearings in subsequent design iterations. This paper describes the new test rig and demonstrates its capabilities through the initial run with a simulated shaft system.

A review of turbulent-boundary-layer heat transfer research at Stanford, 1958-1983

NASA Technical Reports Server (NTRS)

Moffat, R. J.; Kays, W. M.

1984-01-01

For the past 25 years, there has existed in the Thermosciences Laboratory of the Mechanical Engineering Department of Stanford University a research program, primarily experimental, concerned with heat transfer through turbulent boundary layers. In the early phases of the program, the topics considered were the simple zero-pressure-gradient turbulent boundary layer with constant and with varying surface temperature, and the accelerated boundary layer. Later equilibrium boundary layers were considered along with factors affecting the boundary layer, taking into account transpired flows, flows with axial pressure gradients, transpiration, acceleration, deceleration, roughness, full-coverage film cooling, surface curvature, free convection, and mixed convection. A description is provided of the apparatus and techniques used, giving attention to the smooth plate rig, the rough plate rig, the full-coverage film cooling rig, the curvature rig, the concave wall rig, the mixed convection tunnel, and aspects of data reduction and uncertainty analysis.

An Experimental Study of Contaminant Intrusion Through Pipe Cracks

EPA Science Inventory

This report describes a series of experiments conducted at U.S. EPA’s Test and Evaluation Facility in 2013-2014 to study the intrusion of contaminated soil water into a pipe crack during simulated backflow events. A test rig was used consisting of a 3’ x 3’ x 3’ acrylic soil bo...

Oil in the Water, Fire in the Sky: Responding to Technological/Environmental Disasters

ERIC Educational Resources Information Center

Lazarus, Philip J.; Sulkowski, Michael L.

2010-01-01

On April 20, 2010, a massive explosion killed 11 workers on the Deepwater Horizon oil rig. Survivors of this explosion recounted terrifying near-death experiences and mourned the loss of coworkers and friends who had perished. Shock and grief spread through small coastal communities composed mostly of fishers and oil workers. However, this was…

ZEST Flight Test Experiments, Kauai Test Facility, Hawaii

DTIC Science & Technology

1991-07-01

present on KTF/PMRF are 3 the Hawaiian monk seal and the Hawaiian hoary bat. The monk seal (Monachus schauinslandi) has established a colony on Niihau ...feeding and resting offshore of the Nohili Ditch (DOE, 1991). 3 The channel between Kauai and Niihau islands is along the migration route of the

Using Sealed Wells to Measure Water Levels Beneath Streams and Floodplains.

PubMed

Noorduijn, Saskia L; Cook, Peter G; Wood, Cameron; White, Nick

2015-01-01

The design of wells beneath streams and floodplains has often employed with tall standpipes to prevent incursion of surface water into the well during flood events. Here, an approach has been presented to minimise the infrastructure demands in these environments by sealing the well top (e.g., prevent water entering the well) and monitor the total pressure in the water column using an absolute (non-vented) pressure transducer. The sealed well design was tested using a laboratory experiment where the total pressure responses were monitored in both an unsealed and sealed well, while the water level was varied. It is observed that, whether the well is sealed or not, the total pressure at a given depth in the aquifer will be equal to that within the well. This indicates that the sealed well design is a viable alternative to tall standpipes and also facilitates installation of wells beneath streams and floodplains. © 2015, National Ground Water Association.

New drilling rigs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tubb, M.

1981-03-01

Rig builders maintain their frantic work pace to meet drilling contractors' orders for new mobile units - principally the jack-up and semis now so popular. Leading new rig client is Santa Fe Drilling, which has ordered its seventh new offshore unit. The order includes two Enhanced 9500 Pacesetter Series semis, each scheduled to cost $80 million, to be built by Daewoo Shipbuilding and Heavy Machinery in Korea.

Laboratory facility for testing electric-vehicle batteries Test rig for simulating duty cycles with different discharge modes

NASA Astrophysics Data System (ADS)

Hamilton, J. A.; Rand, D. A. J.

1983-03-01

A test rig has been designed and constructed to examine the performance of batteries under laboratory conditions that simulate the power characteristics of electric vehicles. Each station in the rig subjects a battery to continuous charge/discharge cycles, with an equalising charge every eighth cycle. The battery discharge follows the current-verse-time profile of a given vehicle operating under a driving schedule normal to road service. The test rig allows both smooth- and pulsed-current discharge to be investigated. Data collection is accomplished either with multi-pen recorders or with a computer-based information logger.

Regulation of WNT Signaling at the Neuromuscular Junction by the Immunoglobulin Superfamily Protein RIG-3 in Caenorhabditis elegans

PubMed Central

Pandey, Pratima; Bhardwaj, Ashwani; Babu, Kavita

2017-01-01

Perturbations in synaptic function could affect the normal behavior of an animal, making it important to understand the regulatory mechanisms of synaptic signaling. Previous work has shown that in Caenorhabditis elegans an immunoglobulin superfamily protein, RIG-3, functions in presynaptic neurons to maintain normal acetylcholine receptor levels at the neuromuscular junction (NMJ). In this study, we elucidate the molecular and functional mechanism of RIG-3. We demonstrate by genetic and BiFC (Bi-molecular Fluorescence Complementation) assays that presynaptic RIG-3 functions by directly interacting with the immunoglobulin domain of the nonconventional Wnt receptor, ROR receptor tyrosine kinase (RTK), CAM-1, which functions in postsynaptic body-wall muscles. This interaction in turn inhibits Wnt/LIN-44 signaling through the ROR/CAM-1 receptor, and allows for maintenance of normal acetylcholine receptor, AChR/ACR-16, levels at the neuromuscular synapse. Further, this work reveals that RIG-3 and ROR/CAM-1 function through the β-catenin/HMP-2 at the NMJ. Taken together, our results demonstrate that RIG-3 functions as an inhibitory molecule of the Wnt/LIN-44 signaling pathway through the RTK, CAM-1. PMID:28515212

Viral unmasking of cellular 5S rRNA pseudogene transcripts induces RIG-I-mediated immunity.

PubMed

Chiang, Jessica J; Sparrer, Konstantin M J; van Gent, Michiel; Lässig, Charlotte; Huang, Teng; Osterrieder, Nikolaus; Hopfner, Karl-Peter; Gack, Michaela U

2018-01-01

The sensor RIG-I detects double-stranded RNA derived from RNA viruses. Although RIG-I is also known to have a role in the antiviral response to DNA viruses, physiological RNA species recognized by RIG-I during infection with a DNA virus are largely unknown. Using next-generation RNA sequencing (RNAseq), we found that host-derived RNAs, most prominently 5S ribosomal RNA pseudogene 141 (RNA5SP141), bound to RIG-I during infection with herpes simplex virus 1 (HSV-1). Infection with HSV-1 induced relocalization of RNA5SP141 from the nucleus to the cytoplasm, and virus-induced shutoff of host protein synthesis downregulated the abundance of RNA5SP141-interacting proteins, which allowed RNA5SP141 to bind RIG-I and induce the expression of type I interferons. Silencing of RNA5SP141 strongly dampened the antiviral response to HSV-1 and the related virus Epstein-Barr virus (EBV), as well as influenza A virus (IAV). Our findings reveal that antiviral immunity can be triggered by host RNAs that are unshielded following depletion of their respective binding proteins by the virus.

Local infiltration of rabies immunoglobulins without systemic intramuscular administration: An alternative cost effective approach for passive immunization against rabies

PubMed Central

Bharti, Omesh Kumar; Madhusudana, Shampur Narayan; Gaunta, Pyare Lal; Belludi, Ashwin Yajaman

2016-01-01

ABSTRACT Presently the dose of rabies immunoglobulin (RIG) which is an integral part of rabies post exposure prophylaxis (PEP) is calculated based on body weight though the recommendation is to infiltrate the wound(s). This practice demands large quantities of RIG which may be unaffordable to many patients. In this background, we conducted this study to know if the quantity and cost of RIG can be reduced by restricting passive immunization to local infiltration alone and avoiding systemic intramuscular administration based on the available scientific evidence. Two hundred and sixty nine category III patients bitten by suspect or confirmed rabid dogs/animals were infiltrated with equine rabies immunoglobulin (ERIGs) in and around the wound. The quantity of ERIG used was proportionate to the size and number of wounds irrespective of their body weight. They were followed with a regular course of rabies vaccination by intra-dermal route. As against 363 vials of RIGs required for all these cases as per current recommendation based on body weight, they required only 42 vials of 5ml RIG. Minimum dose of RIGs given was 0.25 ml and maximum dose given was 8 ml. On an average 1.26 ml of RIGs was required per patient that costs Rs. 150 ($3). All the patients were followed for 9 months and they were healthy and normal at the end of observation period. With local infiltration, that required small quantities of RIG, the RIGs could be made available to all patients in times of short supply in the market. A total of 30 (11%) serum samples of patients were tested for rabies virus neutralizing antibodies by the rapid fluorescent focus inhibition test (RFFIT) and all showed antibody titers >0.5 IU/mL by day 14. In no case the dose was higher than that required based on body weight and no immunosuppression resulted. To conclude, this pilot study shows that local infiltration of RIG need to be considered in times of non-availability in the market or unaffordability by poor patients. This preliminary study needs to be done on larger scale in other centers with long term follow up to substantiate the results of our study. PMID:26317441

High temperature pressurized high frequency testing rig and test method

DOEpatents

De La Cruz, Jose; Lacey, Paul

2003-04-15

An apparatus is described which permits the lubricity of fuel compositions at or near temperatures and pressures experienced by compression ignition fuel injector components during operation in a running engine. The apparatus consists of means to apply a measured force between two surfaces and oscillate them at high frequency while wetted with a sample of the fuel composition heated to an operator selected temperature. Provision is made to permit operation at or near the flash point of the fuel compositions. Additionally a method of using the subject apparatus to simulate ASTM Testing Method D6079 is disclosed, said method involving using the disclosed apparatus to contact the faces of prepared workpieces under a measured load, sealing the workface contact point into the disclosed apparatus while immersing said contact point between said workfaces in a lubricating media to be tested, pressurizing and heating the chamber and thereby the fluid and workfaces therewithin, using the disclosed apparatus to impart a differential linear motion between the workpieces at their contact point until a measurable scar is imparted to at least one workpiece workface, and then evaluating the workface scar.

USGS/EPA collection protocol for bacterial pathogens in soil

USGS Publications Warehouse

Griffin, Dale W.; Shaefer, F.L.; Charlena Bowling,; Dino Mattorano,; Tonya Nichols,; Erin Silvestri,

2014-01-01

This Sample Collection Procedure (SCP) describes the activities and considerations for the collection of bacterial pathogens from representative surface soil samples (0-5 cm). This sampling depth can be reached without the use of a drill rig, direct-push technology, or other mechanized equipment. This procedure can be used in most soil types but is limited to sampling at or near the ground surface. This protocol has components for two different types of sampling applications: (1) typical sampling, when there is no suspicion of contamination (e.g., surveillance or background studies); and (2) in response to known or suspected accidental contamination (e.g., the presence of animal carcasses). This protocol does not cover sampling in response to a suspected bioterrorist or intentional release event. Surface material is removed to the required depth (0-5 cm) and clean trowel or 50 ml sample tube is used to collect the sample. Sample containers are sealed, bagged, and shipped to the laboratory for analysis. Associated documentation, including a Field Data Log and Chain-of-Custody are also included in this document.

Study of process variables associated with manufacturing hermetically-sealed nickel-cadmium cells

NASA Technical Reports Server (NTRS)

Miller, L.

1972-01-01

The effort and results of a program to determine and study the critical process variables associated with the manufacture of aerospace, hermetically-sealed, nickel-cadmium cells are reported. During the period, the impregnation/polarization process variable study was brought to a close with the completion of a series of related experiments. The results of the experiments are summarized. During this period, a general characterization of cell separator materials was initiated. The major conclusions resulting from the characterization of materials are included.

The autoinhibitory CARD2-Hel2i Interface of RIG-I governs RNA selection.

PubMed

Ramanathan, Anand; Devarkar, Swapnil C; Jiang, Fuguo; Miller, Matthew T; Khan, Abdul G; Marcotrigiano, Joseph; Patel, Smita S

2016-01-29

RIG-I (Retinoic Acid Inducible Gene-I) is a cytosolic innate immune receptor that detects atypical features in viral RNAs as foreign to initiate a Type I interferon signaling response. RIG-I is present in an autoinhibited state in the cytoplasm and activated by blunt-ended double-stranded (ds)RNAs carrying a 5' triphosphate (ppp) moiety. These features found in many pathogenic RNAs are absent in cellular RNAs due to post-transcriptional modifications of RNA ends. Although RIG-I is structurally well characterized, the mechanistic basis for RIG-I's remarkable ability to discriminate between cellular and pathogenic RNAs is not completely understood. We show that RIG-I's selectivity for blunt-ended 5'-ppp dsRNAs is ≈3000 times higher than non-blunt ended dsRNAs commonly found in cellular RNAs. Discrimination occurs at multiple stages and signaling RNAs have high affinity and ATPase turnover rate and thus a high katpase/Kd. We show that RIG-I uses its autoinhibitory CARD2-Hel2i (second CARD-helicase insertion domain) interface as a barrier to select against non-blunt ended dsRNAs. Accordingly, deletion of CARDs or point mutations in the CARD2-Hel2i interface decreases the selectivity from ≈3000 to 150 and 750, respectively. We propose that the CARD2-Hel2i interface is a 'gate' that prevents cellular RNAs from generating productive complexes that can signal. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

Testing for voter rigging in small polling stations

PubMed Central

Jimenez, Raúl; Hidalgo, Manuel; Klimek, Peter

2017-01-01

Nowadays, a large number of countries combine formal democratic institutions with authoritarian practices. Although in these countries the ruling elites may receive considerable voter support, they often use several manipulation tools to control election outcomes. A common practice of these regimes is the coercion and mobilization of large numbers of voters. This electoral irregularity is known as voter rigging, distinguishing it from vote rigging, which involves ballot stuffing or stealing. We develop a statistical test to quantify the extent to which the results of a particular election display traces of voter rigging. Our key hypothesis is that small polling stations are more susceptible to voter rigging because it is easier to identify opposing individuals, there are fewer eyewitnesses, and interested parties might reasonably expect fewer visits from election observers. We devise a general statistical method for testing whether voting behavior in small polling stations is significantly different from the behavior in their neighbor stations in a way that is consistent with the widespread occurrence of voter rigging. On the basis of a comparative analysis, the method enables third parties to conclude that an explanation other than simple variability is needed to explain geographic heterogeneities in vote preferences. We analyze 21 elections in 10 countries and find significant statistical anomalies compatible with voter rigging in Russia from 2007 to 2011, in Venezuela from 2006 to 2013, and in Uganda in 2011. Particularly disturbing is the case of Venezuela, where the smallest polling stations were decisive to the outcome of the 2013 presidential elections. PMID:28695193

Type I IFN triggers RIG-I/TLR3/NLRP3-dependent inflammasome activation in influenza A virus infected cells.

PubMed

Pothlichet, Julien; Meunier, Isabelle; Davis, Beckley K; Ting, Jenny P-Y; Skamene, Emil; von Messling, Veronika; Vidal, Silvia M

2013-01-01

Influenza A virus (IAV) triggers a contagious and potentially lethal respiratory disease. A protective IL-1β response is mediated by innate receptors in macrophages and lung epithelial cells. NLRP3 is crucial in macrophages; however, which sensors elicit IL-1β secretion in lung epithelial cells remains undetermined. Here, we describe for the first time the relative roles of the host innate receptors RIG-I (DDX58), TLR3, and NLRP3 in the IL-1β response to IAV in primary lung epithelial cells. To activate IL-1β secretion, these cells employ partially redundant recognition mechanisms that differ from those described in macrophages. RIG-I had the strongest effect through a MAVS/TRIM25/Riplet-dependent type I IFN signaling pathway upstream of TLR3 and NLRP3. Notably, RIG-I also activated the inflammasome through interaction with caspase 1 and ASC in primary lung epithelial cells. Thus, NS1, an influenza virulence factor that inhibits the RIG-I/type I IFN pathway, strongly modulated the IL-1β response in lung epithelial cells and in ferrets. The NS1 protein derived from a highly pathogenic strain resulted in increased interaction with RIG-I and inhibited type I IFN and IL-1β responses compared to the least pathogenic virus strains. These findings demonstrate that in IAV-infected lung epithelial cells RIG-I activates the inflammasome both directly and through a type I IFN positive feedback loop.

Testing for voter rigging in small polling stations.

PubMed

Jimenez, Raúl; Hidalgo, Manuel; Klimek, Peter

2017-06-01

Nowadays, a large number of countries combine formal democratic institutions with authoritarian practices. Although in these countries the ruling elites may receive considerable voter support, they often use several manipulation tools to control election outcomes. A common practice of these regimes is the coercion and mobilization of large numbers of voters. This electoral irregularity is known as voter rigging, distinguishing it from vote rigging, which involves ballot stuffing or stealing. We develop a statistical test to quantify the extent to which the results of a particular election display traces of voter rigging. Our key hypothesis is that small polling stations are more susceptible to voter rigging because it is easier to identify opposing individuals, there are fewer eyewitnesses, and interested parties might reasonably expect fewer visits from election observers. We devise a general statistical method for testing whether voting behavior in small polling stations is significantly different from the behavior in their neighbor stations in a way that is consistent with the widespread occurrence of voter rigging. On the basis of a comparative analysis, the method enables third parties to conclude that an explanation other than simple variability is needed to explain geographic heterogeneities in vote preferences. We analyze 21 elections in 10 countries and find significant statistical anomalies compatible with voter rigging in Russia from 2007 to 2011, in Venezuela from 2006 to 2013, and in Uganda in 2011. Particularly disturbing is the case of Venezuela, where the smallest polling stations were decisive to the outcome of the 2013 presidential elections.

GUCY2C Signaling Opposes the Acute Radiation-Induced GI Syndrome.

PubMed

Li, Peng; Wuthrick, Evan; Rappaport, Jeff A; Kraft, Crystal; Lin, Jieru E; Marszalowicz, Glen; Snook, Adam E; Zhan, Tingting; Hyslop, Terry M; Waldman, Scott A

2017-09-15

High doses of ionizing radiation induce acute damage to epithelial cells of the gastrointestinal (GI) tract, mediating toxicities restricting the therapeutic efficacy of radiation in cancer and morbidity and mortality in nuclear disasters. No approved prophylaxis or therapy exists for these toxicities, in part reflecting an incomplete understanding of mechanisms contributing to the acute radiation-induced GI syndrome (RIGS). Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. Here, we reveal a role for the GUCY2C paracrine axis in compensatory mechanisms opposing RIGS. Eliminating GUCY2C signaling exacerbated RIGS, amplifying radiation-induced mortality, weight loss, mucosal bleeding, debilitation, and intestinal dysfunction. Durable expression of GUCY2C, guanylin, and uroguanylin mRNA and protein by intestinal epithelial cells was preserved following lethal irradiation inducing RIGS. Oral delivery of the heat-stable enterotoxin (ST), an exogenous GUCY2C ligand, opposed RIGS, a process requiring p53 activation mediated by dissociation from MDM2. In turn, p53 activation prevented cell death by selectively limiting mitotic catastrophe, but not apoptosis. These studies reveal a role for the GUCY2C paracrine hormone axis as a novel compensatory mechanism opposing RIGS, and they highlight the potential of oral GUCY2C agonists (Linzess; Trulance) to prevent and treat RIGS in cancer therapy and nuclear disasters. Cancer Res; 77(18); 5095-106. ©2017 AACR . ©2017 American Association for Cancer Research.

Engagement of Metal Debris into Gear Mesh

NASA Technical Reports Server (NTRS)

handschuh, Robert F.; Krantz, Timothy L.

2010-01-01

A series of bench-top experiments was conducted to determine the effects of metallic debris being dragged through meshing gear teeth. A test rig that is typically used to conduct contact fatigue experiments was used for these tests. Several sizes of drill material, shim stock and pieces of gear teeth were introduced and then driven through the meshing region. The level of torque required to drive the "chip" through the gear mesh was measured. From the data gathered, chip size sufficient to jam the mechanism can be determined.

Segmentation of financial seals and its implementation on a DSP-based system

NASA Astrophysics Data System (ADS)

He, Jin; Liu, Tiegen; Guo, Jingjing; Zhang, Hao

2009-11-01

Automatic seal imprint identification is an important part of modern financial security. Accurate segmentation is the basis of correct identification. In this paper, a DSP (digital signal processor) based identification system was designed, and an adaptive algorithm was proposed to extract binary seal images from financial instruments. As the kernel of the identification system, a DSP chip of TMS320DM642 was used to implement image processing, controlling and coordinating works of each system module. The proposed algorithm consisted of three stages, including extraction of grayscale seal image, denoising and binarization. A grayscale seal image was extracted by color transform from a financial instrument image. Adaptive morphological operations were used to highlight details of the extracted grayscale seal image and smooth the background. After median filter for noise elimination, the filtered seal image was binarized by Otsu's method. The algorithm was developed based on the DSP development environment CCS and real-time operation system DSP/BIOS. To simplify the implementation of the proposed algorithm, the calibration of white balance and the coarse positioning of the seal imprint were implemented by TMS320DM642 controlling image acquisition. IMGLIB of TMS320DM642 was used for the efficiency improvement. The experiment result showed that financial seal imprints, even with intricate and dense strokes can be correctly segmented by the proposed algorithm. Adhesion and incompleteness distortions in the segmentation results were reduced, even when the original seal imprint had a poor quality.

The behavioural response of Australian fur seals to motor boat noise.

PubMed

Tripovich, Joy S; Hall-Aspland, Sophie; Charrier, Isabelle; Arnould, John P Y

2012-01-01

Australian fur seals breed on thirteen islands located in the Bass Strait, Australia. Land access to these islands is restricted, minimising human presence but boat access is still permissible with limitations on approach distances. Thirty-two controlled noise exposure experiments were conducted on breeding Australian fur seals to determine their behavioural response to controlled in-air motor boat noise on Kanowna Island (39°10'S, 146°18'E). Our results show there were significant differences in the seals' behaviour at low (64-70 dB) versus high (75-85 dB) sound levels, with seals orientating themselves towards or physically moving away from the louder boat noise at three different sound levels. Furthermore, seals responded more aggressively with one another and were more alert when they heard louder boat noise. Australian fur seals demonstrated plasticity in their vocal responses to boat noise with calls being significantly different between the various sound intensities and barks tending to get faster as the boat noise got louder. These results suggest that Australian fur seals on Kanowna Island show behavioural disturbance to high level boat noise. Consequently, it is recommended that an appropriate level of received boat sound emissions at breeding fur seal colonies be below 74 dB and that these findings be taken into account when evaluating appropriate approach distances and speed limits for boats.

The Behavioural Response of Australian Fur Seals to Motor Boat Noise

PubMed Central

Tripovich, Joy S.; Hall-Aspland, Sophie; Charrier, Isabelle; Arnould, John P. Y.

2012-01-01

Australian fur seals breed on thirteen islands located in the Bass Strait, Australia. Land access to these islands is restricted, minimising human presence but boat access is still permissible with limitations on approach distances. Thirty-two controlled noise exposure experiments were conducted on breeding Australian fur seals to determine their behavioural response to controlled in-air motor boat noise on Kanowna Island (39°10′S, 146°18′E). Our results show there were significant differences in the seals' behaviour at low (64–70 dB) versus high (75–85 dB) sound levels, with seals orientating themselves towards or physically moving away from the louder boat noise at three different sound levels. Furthermore, seals responded more aggressively with one another and were more alert when they heard louder boat noise. Australian fur seals demonstrated plasticity in their vocal responses to boat noise with calls being significantly different between the various sound intensities and barks tending to get faster as the boat noise got louder. These results suggest that Australian fur seals on Kanowna Island show behavioural disturbance to high level boat noise. Consequently, it is recommended that an appropriate level of received boat sound emissions at breeding fur seal colonies be below 74 dB and that these findings be taken into account when evaluating appropriate approach distances and speed limits for boats. PMID:22623998

Long-term animal experiments with an intraventricular axial flow blood pump.

PubMed

Yamazaki, K; Kormos, R L; Litwak, P; Tagusari, O; Mori, T; Antaki, J F; Kameneva, M; Watach, M; Gordon, L; Mukuo, H; Umezu, M; Tomioka, J; Outa, E; Griffith, B P; Koyanagai, H

1997-01-01

A miniature intraventricular axial flow blood pump (IVAP) is undergoing in vivo evaluation in calves. The IVAP system consists of a miniature (phi 13.9 mm) axial flow pump that resides within the left ventricular (LV) chamber and a brushless DC motor. The pump is fabricated from titanium alloy, and the pump weight is 170 g. It produces a flow rate of over 5 L/min against 100 mmHg pressure at 9,000 rpm with an 8 W total power consumption. The maximum total efficiency exceeds 17%. A purged lip seal system is used in prototype no. 8, and a newly developed "Cool-Seal" (a low temperature mechanical seal) is used in prototype no. 9. In the Cool-Seal system, a large amount of purge flow is introduced behind the seal faces to augment convective heat transfer, keeping the seal face temperature at a low level for prevention of heat denaturation of blood proteins. The Cool-Seal system consumes < 10 cc purge fluid per day and has greatly extended seal life. The pumps were implanted in three calves (26, 30, and 168 days of support). The pump was inserted through a left thoracotomy at the fifth intercostal space. Two pursestring sutures were placed on the LV apex, and the apex was cored with a myocardial punch. The pump was inserted into the LV with the outlet cannula smoothly passing through the aortic valve without any difficulty. Only 5 min elapsed between the time of chest opening and initiation of pumping. Pump function remained stable throughout in all experiments. No cardiac arrhythmias were detected, even at treadmill exercise tests. The plasma free hemoglobin level remained in the acceptable range. Post mortem examination did not reveal any interference between the pump and the mitral apparatus. No major thromboembolism was detected in the vital organs in Cases 1 or 2, but a few small renal infarcts were detected in Case 3.

DOE Office of Scientific and Technical Information (OSTI.GOV)

McCann, D.; White, D.

This paper reports on a smart alarm system installed on a number of offshore rigs and one land rig which can detect kicks more quickly than conventional systems. This rapid kick detection improves rig safety because the smaller the detected influx, the easier it is to control the well. The extensive computerized monitoring system helps drilling personnel detect fluid influxes and fluid losses before the changes in flow would normally be apparent.

The Effect of Applied Tensile Stress on Localized Corrosion in Sensitized AA5083

DTIC Science & Technology

2015-09-01

of stainless steel 4-point bending rig used to apply elastic stress to aluminum plate samples. (Bottom) Stress- strain data based on displacement and...ASTM-G39, from [25]. ..........................20 Figure 13. Photograph of stainless steel 4-point bending rig used to apply elastic stress to...aluminum plate samples, from [8]. ....................................................20 Figure 14. Photograph of stainless steel 4-point bending rig

A numerical model of a HIL scaled roller rig for simulation of wheel-rail degraded adhesion condition

NASA Astrophysics Data System (ADS)

Conti, Roberto; Meli, Enrico; Pugi, Luca; Malvezzi, Monica; Bartolini, Fabio; Allotta, Benedetto; Rindi, Andrea; Toni, Paolo

2012-05-01

Scaled roller rigs used for railway applications play a fundamental role in the development of new technologies and new devices, combining the hardware in the loop (HIL) benefits with the reduction of the economic investments. The main problem of the scaled roller rig with respect to the full scale ones is the improved complexity due to the scaling factors. For this reason, before building the test rig, the development of a software model of the HIL system can be useful to analyse the system behaviour in different operative conditions. One has to consider the multi-body behaviour of the scaled roller rig, the controller and the model of the virtual vehicle, whose dynamics has to be reproduced on the rig. The main purpose of this work is the development of a complete model that satisfies the previous requirements and in particular the performance analysis of the controller and of the dynamical behaviour of the scaled roller rig when some disturbances are simulated with low adhesion conditions. Since the scaled roller rig will be used to simulate degraded adhesion conditions, accurate and realistic wheel-roller contact model also has to be included in the model. The contact model consists of two parts: the contact point detection and the adhesion model. The first part is based on a numerical method described in some previous studies for the wheel-rail case and modified to simulate the three-dimensional contact between revolute surfaces (wheel-roller). The second part consists in the evaluation of the contact forces by means of the Hertz theory for the normal problem and the Kalker theory for the tangential problem. Some numerical tests were performed, in particular low adhesion conditions were simulated, and bogie hunting and dynamical imbalance of the wheelsets were introduced. The tests were devoted to verify the robustness of control system with respect to some of the more frequent disturbances that may influence the roller rig dynamics. In particular we verified that the wheelset imbalance could significantly influence system performance, and to reduce the effect of this disturbance a multistate filter was designed.

Hemostasis and sealing of air leaks in the lung using high-intensity focused ultrasound.

PubMed

Vaezy, Shahram; Zderic, Vesna; Karmy-Jones, Riyad; Jurkovich, Gregory J; Cornejo, Carol; Martin, Roy W

2007-06-01

Operative management of parenchymal lung injury can be complicated by persistent hemorrhage and air leak, which might require resection. Techniques that preserve parenchyma are associated with improved survival. High-intensity focused ultrasound (HIFU) has been demonstrated as a useful method for hemostasis in experimental solid organ injuries. We wished to investigate whether this could be applied to lung injuries. An intraoperative HIFU device (frequency of 5.7 MHz, acoustic power of 65 W), equipped with a titanium coupler, was used. Incisions (average length of 2.5 cm, and depth of 5 mm) were made in the lungs of 11 pigs, which created both parenchymal hemorrhage and air leakage. In treatment experiments, 70 incisions were sealed with HIFU. The HIFU application started within 10 seconds of inducing the injury. Hemostasis was assessed by visual observation of sealed incisions. The possible air leakage was determined by submersing the sealed incision under the layer of water and observing for air bubble formation. In control experiments, five incisions were left untreated to monitor air leaks and bleeding for 2 minutes. Hemostasis and pneumostasis (sealing of air leaks) of the treated incisions were achieved in 51 +/- 37 seconds (mean +/- SD) (range of 10-210 seconds) of HIFU application time. Over 95% of incisions were hemostatic within 2 minutes of HIFU application. The treatment time was not dependent on the incision length or depth. In control experiments, the air leaking and bleeding were still present at 2 minutes after the injury. Intraoperative HIFU might provide an effective method of hemostasis and control of air leaks from lacerations caused by trauma.

Seawater drinking restores water balance in dehydrated harp seals.

PubMed

How, Ole-Jakob; Nordøy, Erling S

2007-07-01

The purpose of this study was to answer the question of whether dehydrated harp seals (Phoca groenlandica) are able to obtain a net gain of water from the intake of seawater. Following 24 h of fasting, three subadult female harp seals were dehydrated by intravenous administration of the osmotic diuretic, mannitol. After another 24 h of fasting, the seals were given 1,000 ml seawater via a stomach tube. Urine and blood were collected for measurement of osmolality and osmolytes, while total body water (TBW) was determined by injections of tritiated water. In all seals, the maximum urinary concentrations of Na(+) and Cl(-) were higher than in seawater, reaching 540 and 620 mM, respectively, compared to 444 and 535 mM in seawater. In another experiment, the seals were given ad lib access to seawater for 48 h after mannitol-induced hyper-osmotic dehydration. In animals without access to seawater, the mean blood osmolality increased from 331 to 363 mOsm kg(-1) during dehydration. In contrast, the blood osmolality, hematocrit and TBW returned to normal when the seals were permitted ad lib access to seawater after dehydration. In conclusion, this study shows that harp seals have the capacity to gain net water from mariposa (voluntarily drinking seawater) and are able to restore water balance after profound dehydration by drinking seawater.

A modeling study on the thermomechanical behavior of glass-ceramic and self-healing glass seals at elevated temperatures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Govindaraju, Nirmal; Liu, Wenning N.; Sun, Xin

Hermetic gas seals are critical components for planar solid oxide fuel cells. This article focuses on comparative evaluation of a glass-ceramic developed by the Pacific Northwest National Laboratory (PNNL) and a self-healing glass seal developed by the University of Cincinnati. The stress and strain levels in the Positive electrode-Electrolyte-Negative electrode (PEN) seal in one cell stack are evaluated using a multi-physics simulation package developed at PNNL. Simulations were carried out with and without consideration of clamping force and stack body force, respectively. The results indicate that the overall stress and strain levels are dominated by the thermal expansion mismatches betweenmore » the different cell components. Further, compared with glass-ceramic seal, the self-healing glass seal results in much lower steady state stress due to its much lower stiffness at the operating temperature of SOFC, and also exhibits much shorter relaxation times due to high creep rate. It is also noted that the self-healing glass seal will experience continuing creep deformation under the operating temperature of SOFC therefore resulting in possible overflow of the sealing materials. Further stopper material may need to be added to maintain its geometric stability during operation.« less

The Design and Analysis of the Hydraulic-pressure Seal of the Engine Box

NASA Astrophysics Data System (ADS)

Chen, Zhenya; Shen, Xingquan; Xin, Zhijie; Guo, Tingting; Liao, Kewei

2017-12-01

According to the sealing requirements of engine casing, using NX software to establish three-dimensional solid model of the engine box. Designing two seals suppress schemes basing on analyzing the characteristics of the case structure, one of seal is using two pins on one side to localize, the other is using cylinder to top tight and fasten, Clarifying the reasons for the using the former scheme have a lower cost. At the same time analysesing of the forces and deformation of the former scheme using finite element analysis software and the NX software, results proved that the pressure scheme can meet the actual needs of the program. It illustrated the composition of the basic principles of manual pressure and hydraulic system, verifed the feasibility of the seal program using experiment, providing reference for the experimental program of hydrostatic pressure in the future.

A memory like a female Fur Seal: long-lasting recognition of pup's voice by mothers.

PubMed

Mathevon, Nicolas; Charrier, Isabelle; Aubin, Thierry

2004-06-01

In colonial mammals like fur seals, mutual vocal recognition between mothers and their pup is of primary importance for breeding success. Females alternate feeding sea-trips with suckling periods on land, and when coming back from the ocean, they have to vocally find their offspring among numerous similar-looking pups. Young fur seals emit a 'mother-attraction call' that presents individual characteristics. In this paper, we review the perceptual process of pup's call recognition by Subantarctic Fur Seal Arctocephalus tropicalis mothers. To identify their progeny, females rely on the frequency modulation pattern and spectral features of this call. As the acoustic characteristics of a pup's call change throughout the lactation period due to the growing process, mothers have thus to refine their memorization of their pup's voice. Field experiments show that female Fur Seals are able to retain all the successive versions of their pup's call.

Sealed Silver-oxide Cadmium Batteries for Space Flight, 1960 - 1977

NASA Technical Reports Server (NTRS)

Hennigan, Thomas J.

1978-01-01

A technical summary of design, development, and test activities with Silver-Oxide Cadmium Batteries at the Goddard Space Flight Center since 1960 is given. The flight experience of over 15 missions has demonstrated the sealed Silver-Oxide Cadmium Battery to be a viable energy storage device for missions requiring ultra-clean magnetic environment.

Romanian Experience in The Conditioning of Radium Sources

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dogaru, Gh.; Dragolici, F.; Rotarescu, Gh.

2008-07-01

Ra{sup 226} first radionuclide separated from pitchblende in 1898 by Pierre and Marie Curie was successfully used in medicine, industry as in other fields being the only one available radionuclide till 1940 when were produced other radionuclides in accelerators. On long term the use of Ra{sup 226} sealed sources are not any more safe due to: the high specific activity, long half live, decays in Rn{sup 226} gas which increases the internal pressure of capsule leading in time to the leakage, the salts as raw materials from which the sealed sources are manufactured are soluble, there is a leak ofmore » information and records on the manufacture and operation. Based on this consideration in Romania regulatory authority did not authorized any more the use of these sealed sources [1]. The paper presents some aspects from Romanian experience related to the collection and conditioning of radium sealed sources. Data relating the radium inventory as well as the arrangements made in order to create a workshop for the conditioning of radium sources are presented. (authors)« less

Overcoming codon bias: a method for high-level overexpression of Plasmodium and other AT-rich parasite genes in Escherichia coli.

PubMed

Baca, A M; Hol, W G

2000-02-01

Parasite genes often use codons which are rarely used in the highly expressed genes of Escherichia coli, possibly resulting in translational stalling and lower yields of recombinant protein. We have constructed the "RIG" plasmid to overcome the potential codon-bias problem seen in Plasmodium genes. RIG contains the genes that encode three tRNAs (Arg, Ile, Gly), which recognise rare codons found in parasite genes. When co-transformed into E. coli along with expression plasmids containing parasite genes, RIG can greatly increase levels of overexpressed protein. Codon frequency analysis suggests that RIG may be applied to a variety of protozoan and helminth genes.

Energy efficient engine: High pressure turbine uncooled rig technology report

NASA Technical Reports Server (NTRS)

Gardner, W. B.

1979-01-01

Results obtained from testing five performance builds (three vane cascades and two rotating rigs of the Energy Efficient Engine uncooled rig have established the uncooled aerodynamic efficiency of the high-pressure turbine at 91.1 percent. This efficiency level was attained by increasing the rim speed and annulus area (AN(2)), and by increasing the turbine reaction level. The increase in AN(2) resulted in a performance improvement of 1.15 percent. At the design point pressure ratio, the increased reaction level rig demonstrated an efficiency of 91.1 percent. The results of this program have verified the aerodynamic design assumptions established for the Energy Efficient Engine high-pressure turbine component.

Stratified charge rotary aircraft engine technology enablement program

NASA Technical Reports Server (NTRS)

Badgley, P. R.; Irion, C. E.; Myers, D. M.

1985-01-01

The multifuel stratified charge rotary engine is discussed. A single rotor, 0.7L/40 cu in displacement, research rig engine was tested. The research rig engine was designed for operation at high speeds and pressures, combustion chamber peak pressure providing margin for speed and load excursions above the design requirement for a high is advanced aircraft engine. It is indicated that the single rotor research rig engine is capable of meeting the established design requirements of 120 kW, 8,000 RPM, 1,379 KPA BMEP. The research rig engine, when fully developed, will be a valuable tool for investigating, advanced and highly advanced technology components, and provide an understanding of the stratified charge rotary engine combustion process.

Performance Evaluation of the NASA GTX RBCC Flowpath

NASA Technical Reports Server (NTRS)

Thomas, Scott R.; Palac, Donald T.; Trefny, Charles J.; Roche, Joseph M.

2001-01-01

The NASA Glenn Research Center serves as NASAs lead center for aeropropulsion. Several programs are underway to explore revolutionary airbreathing propulsion systems in response to the challenge of reducing the cost of space transportation. Concepts being investigated include rocket-based combined cycle (RBCC), pulse detonation wave, and turbine-based combined cycle (TBCC) engines. The GTX concept is a vertical launched, horizontal landing, single stage to orbit (SSTO) vehicle utilizing RBCC engines. The propulsion pod has a nearly half-axisymmetric flowpath that incorporates a rocket and ram-scramjet. The engine system operates from lift-off up to above Mach 10, at which point the airbreathing engine flowpath is closed off, and the rocket alone powers the vehicle to orbit. The paper presents an overview of the research efforts supporting the development of this RBCC propulsion system. The experimental efforts of this program consist of a series of test rigs. Each rig is focused on development and optimization of the flowpath over a specific operating mode of the engine. These rigs collectively establish propulsion system performance over all modes of operation, therefore, covering the entire speed range. Computational Fluid Mechanics (CFD) analysis is an important element of the GTX propulsion system development and validation. These efforts guide experiments and flowpath design, provide insight into experimental data, and extend results to conditions and scales not achievable in ground test facilities. Some examples of important CFD results are presented.

Impact of Micro-to Meso-scale Fractures on Sealing Behavior of Argillaceous Cap Rocks For CO 2 Sequestration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Evans, James

This multi-disciplinary project evaluated seal lithologies for the safety and security of long-term geosequestration of CO 2. We used integrated studies to provide qualitative risk for potential seal failure; we integrated data sets from outcrop, core, geochemical analysis, rock failure properties from mechanical testing, geophysical wireline log analysis, and geomechanical modeling to understand the effects of lithologic heterogeneity and changing mechanical properties have on the mechanical properties of the seal. The objectives of this study were to characterize cap rock seals using natural field analogs, available drillhole logging data and whole-rock core, geochemical and isotopic analyses. Rock deformation experiments weremore » carried out on collected samples to develop better models of risk estimation for potential cap rock seal failure. We also sampled variably faulted and fractured cap rocks to examine the impacts of mineralization and/or alteration on the mechanical properties. We compared CO 2 reacted systems to non-CO 2 reacted seal rock types to determine response of each to increased pore fluid pressures and potential for the creation of unintentional hydrofractures at depth.« less

Vibrio vulnificus quorum-sensing molecule cyclo(Phe-Pro) inhibits RIG-I-mediated antiviral innate immunity.

PubMed

Lee, Wooseong; Lee, Seung-Hoon; Kim, Minwoo; Moon, Jae-Su; Kim, Geon-Woo; Jung, Hae-Gwang; Kim, In Hwang; Oh, Ji Eun; Jung, Hi Eun; Lee, Heung Kyu; Ku, Keun Bon; Ahn, Dae-Gyun; Kim, Seong-Jun; Kim, Kun-Soo; Oh, Jong-Won

2018-04-23

The recognition of pathogen-derived ligands by pattern recognition receptors activates the innate immune response, but the potential interaction of quorum-sensing (QS) signaling molecules with host anti-viral defenses remains largely unknown. Here we show that the Vibrio vulnificus QS molecule cyclo(Phe-Pro) (cFP) inhibits interferon (IFN)-β production by interfering with retinoic-acid-inducible gene-I (RIG-I) activation. Binding of cFP to the RIG-I 2CARD domain induces a conformational change in RIG-I, preventing the TRIM25-mediated ubiquitination to abrogate IFN production. cFP enhances susceptibility to hepatitis C virus (HCV), as well as Sendai and influenza viruses, each known to be sensed by RIG-I but did not affect the melanoma-differentiation-associated gene 5 (MDA5)-recognition of norovirus. Our results reveal an inter-kingdom network between bacteria, viruses and host that dysregulates host innate responses via a microbial quorum-sensing molecule modulating the response to viral infection.

Influenza A virus NS1 targets the ubiquitin ligase TRIM25 to evade recognition by RIG-I

PubMed Central

Gack, Michaela Ulrike; Albrecht, Randy Allen; Urano, Tomohiko; Inn, Kyung-Soo; Huang, I-Chueh; Carnero, Elena; Farzan, Michael; Inoue, Satoshi; Jung, Jae Ung; García-Sastre, Adolfo

2009-01-01

SUMMARY TRIM25 mediates Lys 63-linked ubiquitination of the N-terminal CARDs of the viral RNA sensor RIG-I, leading to type I interferon (IFN) production. Here, we report that the influenza A virus non-structural protein 1 (NS1) specifically inhibits TRIM25-mediated RIG-I CARD ubiquitination, thereby suppressing RIG-I signal transduction. A novel domain in NS1 comprising E96/E97 residues mediates its interaction with the coiled-coil domain of TRIM25, thus blocking TRIM25 multimerization and RIG-I CARD ubiquitination. Furthermore, a recombinant influenza A virus expressing an E96A/E97A NS1 mutant is defective in blocking TRIM25-mediated anti-viral IFN response and loses virulence in mice. Our findings reveal a novel mechanism of influenza virus to inhibit host IFN response and also emphasize the vital role of TRIM25 in modulating viral infections. PMID:19454348

Influenza A virus NS1 targets the ubiquitin ligase TRIM25 to evade recognition by the host viral RNA sensor RIG-I.

PubMed

Gack, Michaela Ulrike; Albrecht, Randy Allen; Urano, Tomohiko; Inn, Kyung-Soo; Huang, I-Chueh; Carnero, Elena; Farzan, Michael; Inoue, Satoshi; Jung, Jae Ung; García-Sastre, Adolfo

2009-05-08

The ubiquitin ligase TRIM25 mediates Lysine 63-linked ubiquitination of the N-terminal CARD domains of the viral RNA sensor RIG-I to facilitate type I interferon (IFN) production and antiviral immunity. Here, we report that the influenza A virus nonstructural protein 1 (NS1) specifically inhibits TRIM25-mediated RIG-I CARD ubiquitination, thereby suppressing RIG-I signal transduction. A novel domain in NS1 comprising E96/E97 residues mediates its interaction with the coiled-coil domain of TRIM25, thus blocking TRIM25 multimerization and RIG-I CARD domain ubiquitination. Furthermore, a recombinant influenza A virus expressing an E96A/E97A NS1 mutant is defective in blocking TRIM25-mediated antiviral IFN response and loses virulence in mice. Our findings reveal a mechanism by which influenza virus inhibits host IFN response and also emphasize the vital role of TRIM25 in modulating antiviral defenses.

ECSIT bridges RIG-I-like receptors to VISA in signaling events of innate antiviral responses.

PubMed

Lei, Cao-Qi; Zhang, Yu; Li, Mi; Jiang, Li-Qun; Zhong, Bo; Kim, Yong Ho; Shu, Hong-Bing

2015-01-01

Upon binding to RNA structures from invading viruses, RIG-I and MDA5 are recruited to mitochondria to interact with VISA and initiate antiviral type I interferon (IFN) responses. How this process is mediated is less understood. In this report, we demonstrate that ECSIT is an essential scaffolding protein that mediates the association of VISA and RIG-I or MDA5. Overexpression of ECSIT potentiated virus-triggered activation of IFN-regulatory factor 3 (IRF3) and expression of IFNB1, whereas knockdown of ECSIT impaired viral infection-induced activation of IRF3 and expression of IFNB1 as well as cellular antiviral responses. Mechanistically, ECSIT was associated with VISA on mitochondria and important for bridging RIG-I and MDA5 to VISA. Our findings suggest that ECSIT mediates virus-triggered type I IFN induction by bridging RIG-I and MDA5 to the VISA complex, and provide new insights into the molecular events of innate antiviral immune responses. © 2014 S. Karger AG, Basel.

Cholera on a Gulf Coast oil rig.

PubMed

Johnston, J M; Martin, D L; Perdue, J; McFarland, L M; Caraway, C T; Lippy, E C; Blake, P A

1983-09-01

A single case of severe diarrhea on a floating Texas oil rig was followed two days later by what proved to be the largest outbreak of cholera in the United States in over a century. After isolation of toxigenic Vibrio cholerae El Tor Inaba of the typical United States phage type from the index patient's stool, the ensuing investigation detected 14 additional cases of cholera and one asymptomatic infection serologically. Infection was associated with eating rice on the oil rig on a particular day (P = 0.03) when an open valve permitted the rig's drinking-water system to be contaminated by canal water containing sewage (including that from the index patient) discharged from the rig. The rice had been rinsed in the contaminated water after cooking, and before being served it had been maintained at a temperature that allows V. cholerae 01 to multiply. Toxigenic V. cholerae 01 is persisting in the United States, and large common-source outbreaks of cholera can occur if proper sanitation is not maintained.

Experimental data and model for the turbulent boundary layer on a convex, curved surface

NASA Technical Reports Server (NTRS)

Gillis, J. C.; Johnson, J. P.; Moffat, R. J.; Kays, W. M.

1981-01-01

Experiments were performed to determine how boundary layer turbulence is affected by strong convex curvature. The data gathered on the behavior of the Reynolds stress suggested the formulation of a simple turbulence model. Data were taken on two separate facilities. Both rigs had flow from a flat surface, over a convex surface with 90 deg of turning and then onto a flat recovery surface. The geometry was adjusted so that, for both rigs, the pressure gradient along the test surface was zero. Two experiments were performed at delta/R approximately 0.10, and one at weaker curvature with delta/R approximately 0.05. Results show that after a sudden introduction of curvature the shear stress in the outer part of the boundary layer is sharply diminished and is even slightly negative near the edge. The wall shear also drops off quickly downstream. When the surface suddenly becomes flat again, the wall shear and shear stress profiles recover very slowly towards flat wall conditions. A simple turbulence model, which was based on the theory that the Prandtl mixing length in the outer layer should scale on the velocity gradient layer, was shown to account for the slow recovery.

STS-47 MS Davis and Pilot Brown repair ISAIAH humidity problem aboard OV-105

NASA Image and Video Library

1992-09-20

STS047-35-022 (12 - 20 Sept 1992) --- Astronauts Curtis L. Brown, Jr., pilot, and N. Jan Davis, mission specialist, team up to cure a high humidity problem in the hornet experiment in the Spacelab-J Science Module of the Earth-orbiting Space Shuttle Endeavour. Via a jury-rigged hose hook-up, the two were able to blow air from a spacesuit fan into the experiment, thus eliminating condensation that obscured the viewing of the Israeli hornet experiment. The experiment examined the effects of microgravity on the orientation, reproductive capability and social activity of 180 female Oriental Hornets.

Oil rigs and offshore sport fishing in Louisiana

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dugas, R.; Guillory, V.; Fischer, M.

Forty years ago, offshore sport fishing in Louisiana was almost nonexistent. Offshore oil drilling platforms are the primary cause of the present increase in sport fishing in this area. Algae and other organisms forming the first step in the food chain cluster around the subsurface structures of the rigs, attracting fish that seek food and shelter. Major game species frequenting these rigs are identified. (3 photos, 22 references, 2 tables)

Burner Rig Laboratory

NASA Image and Video Library

2015-05-12

The Fuel Burner Rig is a test laboratory at NASA Glenn, which subjects new jet engine materials, treated with protective coatings, to the hostile, high temperature, high velocity environment found inside aircraft turbine engines. These samples face 200-mile per hour flames to simulate the temperatures of aircraft engines in flight. The rig can also simulate aircraft carrier and dusty desert operations where salt and sand can greatly reduce engine life and performance.

New drilling rigs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tubb, M.

1981-02-01

Friede and Goldman Ltd. of New Orleans, Louisiana has a successful drilling rig, the L-780 jack-up series. The triangular-shaped drilling vessel measures 180 x 176 ft. and is equipped with three 352 ft legs including spud cans. It is designed to work in up to 250 ft waters and drill to 20,000 ft depths. The unit is scheduled to begin initial drilling operations in the Gulf of Mexico for Arco. Design features are included for the unit. Davie Shipbuilding Ltd. has entered the Mexican offshore market with the signing of a $40,000,000 Canadian contract for a jack-up to work inmore » 300 ft water depths. Baker Marine Corporation has contracted with the People's Republic of China for construction of two self-elevating jack-ups. The units will be built for Magnum Marine, headquartered in Houston. Details for the two rigs are given. Santa Fe International Corporation has ordered a new jack-up rig to work initially in the Gulf of Suez. The newly ordered unit, Rig 136, will be the company's fourth offshore drilling rig now being built in the Far East. Temple Drilling Company has signed a construction contract with Bethlehem Steel for a jack-up to work in 200 ft water depths. Penrod Drilling Company has ordered two additional cantilever type jack-ups for Hitachi Shipbuilding and Engineering Co. Ltd. of Japan. Two semi-submersibles, capable of working in up to 2000 ft water depths, have been ordered by two Liberian companies. Details for these rigs are included. (DP)« less

Product Module Rig Test

NASA Technical Reports Server (NTRS)

Holdeman, James D. (Technical Monitor); Chiappetta, Louis, Jr.; Hautman, Donald J.; Ols, John T.; Padget, Frederick C., IV; Peschke, William O. T.; Shirley, John A.; Siskind, Kenneth S.

2004-01-01

The low emissions potential of a Rich-Quench-Lean (RQL) combustor for use in the High Speed Civil Transport (HSCT) application was evaluated as part of Work Breakdown Structure (WBS) 1.0.2.7 of the NASA Critical Propulsion Components (CPC) Program under Contract NAS3-27235. Combustion testing was conducted in cell 1E of the Jet Burner Test Stand at United Technologies Research Center. Specifically, a Rich-Quench-Lean combustor, utilizing reduced scale quench technology implemented in a quench vane concept in a product-like configuration (Product Module Rig), demonstrated the capability of achieving an emissions index of nitrogen oxides (NOx EI) of 8.5 gm/Kg fuel at the supersonic flight condition (relative to the program goal of 5 gm/Kg fuel). Developmental parametric testing of various quench vane configurations in the more fundamental flametube, Single Module Rig Configuration, demonstrated NOx EI as low as 5.2. All configurations in both the Product Module Rig configuration and the Single Module Rig configuration demonstrated exceptional efficiencies, greater than 99.95 percent, relative to the program goal of 99.9 percent efficiency at supersonic cruise conditions. Sensitivity of emissions to quench orifice design parameters were determined during the parametric quench vane test series in support of the design of the Product Module Rig configuration. For the rectangular quench orifices investigated, an aspect ratio (length/width) of approximately 2 was found to be near optimum. An optimum for orifice spacing was found to exist at approximately 0.167 inches, resulting in 24 orifices per side of a quench vane, for the 0.435 inch quench zone channel height investigated in the Single Module Rig. Smaller quench zone channel heights appeared to be beneficial in reducing emissions. Measurements were also obtained in the Single Module Rig configuration on the sensitivity of emissions to the critical combustor parameters of fuel/air ratio, pressure drop, and residence time. Minimal sensitivity was observed for all of these parameters.

Cancer therapies activate RIG-I-like receptor pathway through endogenous non-coding RNAs

PubMed Central

Ranoa, Diana Rose E.; Parekh, Akash D.; Pitroda, Sean P.; Huang, Xiaona; Darga, Thomas; Wong, Anthony C.; Huang, Lei; Andrade, Jorge; Staley, Jonathan P.; Satoh, Takashi; Akira, Shizuo

2016-01-01

Emerging evidence indicates that ionizing radiation (IR) and chemotherapy activate Type I interferon (IFN) signaling in tumor and host cells. However, the mechanism of induction is poorly understood. We identified a novel radioprotective role for the DEXH box RNA helicase LGP2 (DHX58) through its suppression of IR-induced cytotoxic IFN-beta [1]. LGP2 inhibits activation of the RIG-I-like receptor (RLR) pathway upon binding of viral RNA to the cytoplasmic sensors RIG-I (DDX58) and MDA5 (IFIH1) and subsequent IFN signaling via the mitochondrial adaptor protein MAVS (IPS1). Here we show that MAVS is necessary for IFN-beta induction and interferon-stimulated gene expression in the response to IR. Suppression of MAVS conferred radioresistance in normal and cancer cells. Germline deletion of RIG-I, but not MDA5, protected mice from death following total body irradiation, while deletion of LGP2 accelerated the death of irradiated animals. In human tumors depletion of RIG-I conferred resistance to IR and different classes of chemotherapy drugs. Mechanistically, IR stimulated the binding of cytoplasmic RIG-I with small endogenous non-coding RNAs (sncRNAs), which triggered IFN-beta activity. We demonstrate that the small nuclear RNAs U1 and U2 translocate to the cytoplasm after IR treatment, thus stimulating the formation of RIG-I: RNA complexes and initiating downstream signaling events. Taken together, these findings suggest that the physiologic responses to radio-/chemo-therapy converge on an antiviral program in recruitment of the RLR pathway by a sncRNA-dependent activation of RIG-I which commences cytotoxic IFN signaling. Importantly, activation of interferon genes by radiation or chemotherapy is associated with a favorable outcome in patients undergoing treatment for cancer. To our knowledge, this is the first demonstration of a cell-intrinsic response to clinically relevant genotoxic treatments mediated by an RNA-dependent mechanism. PMID:27034163

Friction and wear of several compressor gas-path seal movements

NASA Technical Reports Server (NTRS)

Bill, R. C.; Wisander, D. W.

1978-01-01

Rub interaction experiments were conducted on a series of sintered and plasma sprayed compressor gas path seal materials in contact with Ti-6Al-4V blade tip and knife edge rotors. The most rub tolerant materials investigated were sintered Nichrome and plasma sprayed nickel 25 percent graphite. The effectiveness of providing a compliant substrate for dense seal material coatings was also demonstrated. In general, it was observed that rotor wear and high frictional energy generation rates accompanied smearing or surface densification of the materials investigated. The onset of smearing was sensitive to rub interaction parameters and seal geometry. Two complementary models were proposed to account for the smearing trends. One is based on thermal effects, the other on particulate escape effects. They were shown to be consistent with the experimental evidence at hand, and together they predict that smearing, with the onset of high energy rub conditions, is favored when incursion rates (radial motion) are low, incursion depths are high, the seal geometry is of a knife-edge character, and the seal particle size is small.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mehra, S.; Bryce, T.

Sedco Forex has recently completed a new land drilling rig, currently deployed in Gabon, that integrates well construction activities with multiskilling to create cost savings across the board in drilling operations. Historically, operators have produced a comprehensive tender package specifying strictly the type and size of individual rig components and the number of personnel required to drill. In this case, the drilling contractor provides a fit-for-purpose rig, consistent with field location, well profile, operator`s priorities, and local constraints.

Effects of surface chemistry on hot corrosion life

NASA Technical Reports Server (NTRS)

Fryxell, R. E.; Leese, G. E.

1985-01-01

This program has its primary objective: the development of hot corrosion life prediction methodology based on a combination of laboratory test data and evaluation of field service turbine components which show evidence of hot corrosion. The laboratory program comprises burner rig testing by TRW. A summary of results is given for two series of burner rig tests. The life prediction methodology parameters to be appraised in a final campaign of burner rig tests are outlined.

Gulf of Mexico and Caribbean Sea Data and Model Base Report

DTIC Science & Technology

1979-07-01

The source levels and spectral characteristics of merchant ships, drill rigs, and seismic profiling sources are reason- ably well known. Lacking...better data, fishing vessels are assumed to be 10 dB quieter than merchar• ships; production platforms are assumed to be similar to drill rigs, corrected...scope of the problem presented by production platforms, mobile drill rigs, and seismic profilers. 5. Impact on Exercise Planning Offshore oil industry

Amino Acid Requirements for MDA5 and LGP2 Recognition by Paramyxovirus V Proteins: a Single Arginine Distinguishes MDA5 from RIG-I

PubMed Central

Rodriguez, Kenny R.

2013-01-01

Paramyxovirus V proteins bind to MDA5 (melanoma differentiation-associated gene 5) and LGP2 (laboratory of genetics and physiology gene 2) but not RIG-I (retinoic acid-inducible gene I). The results demonstrate MDA5 R806 is essential for inhibition by diverse V proteins. Complementary substitution for the analogous RIG-I L714 confers V protein recognition. The analogous LGP2 R455 is required for recognition by measles V protein, but not other V proteins. These findings indicate that paramyxoviruses use a single amino acid to distinguish MDA5 from RIG-I and have evolved distinct contact sites for LGP2 interference. PMID:23269789

Development of advanced seal verification

NASA Technical Reports Server (NTRS)

Workman, Gary L.; Kosten, Susan E.; Abushagur, Mustafa A.

1992-01-01

The purpose of this research is to develop a technique to monitor and insure seal integrity with a sensor that has no active elements to burn-out during a long duration activity, such as a leakage test or especially during a mission in space. The original concept proposed is that by implementing fiber optic sensors, changes in the integrity of a seal can be monitored in real time and at no time should the optical fiber sensor fail. The electrical components which provide optical excitation and detection through the fiber are not part of the seal; hence, if these electrical components fail, they can be easily changed without breaking the seal. The optical connections required for the concept to work does present a functional problem to work out. The utility of the optical fiber sensor for seal monitoring should be general enough that the degradation of a seal can be determined before catastrophic failure occurs and appropriate action taken. Two parallel efforts were performed in determining the feasibility of using optical fiber sensors for seal verification. In one study, research on interferometric measurements of the mechanical response of the optical fiber sensors to seal integrity was studied. In a second study, the implementation of the optical fiber to a typical vacuum chamber was implemented and feasibility studies on microbend experiments in the vacuum chamber were performed. Also, an attempt was made to quantify the amount of pressure actually being applied to the optical fiber using finite element analysis software by Algor.

Studies of soft x-ray transmission through grid supported CH layers

NASA Astrophysics Data System (ADS)

Davis, J. S.; Keiter, P. A.; Klein, S. R.; Frank, Y.; Drake, R. P.; Shvarts, D.

2017-10-01

Recent experiments have shown that it may be possible to use laser-heated high-Z foils to drive new radiation transport (RadTran) experiments in gas fill tubes. These tubes must be pressurized above 1atm and the x-ray source needs to be physically separated from the gas. To achieve this, a grid-supported CH seal is implemented. The grid reduces the total surface area of the gas-seal interaction region lowering the thickness requirements for the CH layer. However, as mesh spacing is reduced, hole closure from wire ablation may reduce the x-ray flux. To optimize the seal design, experiments were performed measuring x-ray transmission through CH layers supported by meshes composed of copper, gold, or stainless steel and using hexagonal or square mesh geometries. The x-ray source was formed by heating a 0.5 μm thick planar gold foil with a 4 ns laser pulse at an intensity of 2 ×1014 W / cm 2. Emission data was collected using an x-ray framing camera and a Dante photodiode array. Experiments show that the CH layers can reach effective temperatures of nearly 100 eV but mesh design significantly affects performance, with a nearly 20 eV difference between the best and worst performing seal targets. This talk will discuss our findings and their impact on future RadTran experiments. This work is funded by the U.S. DOE, through the NNSA-DS and SC-OFES Joint Program in HED Laboratory Plasmas, Grant Number DE-NA0001840, the National LUFP, Grant Number DE-NA0000850, and through NNSA/OICF under Cooperatvie Agreement No. DE-FC52-08NA2830.

Development of a local anesthetic lidocaine-loaded redox-active injectable gel for postoperative pain management.

PubMed

Nagasaki, Yukio; Mizukoshi, Yutaro; Gao, Zhenyu; Feliciano, Chitho P; Chang, Kyungho; Sekiyama, Hiroshi; Kimura, Hiroyuki

2017-07-15

Although local anesthesia is commonly applied for pain relief, there are several issues such as its short duration of action and low effectiveness at the areas of inflammation due to the acidic pH. The presence of excessive amount of reactive oxygen species (ROS) is known to induce inflammation and aggravate pain. To resolve these issues, we developed a redox-active injectable gel (RIG) with ROS-scavenging activity. RIG was prepared by mixing polyamine-b-poly(ethylene glycol)-b-polyamine with nitroxide radical moieties as side chains on the polyamine segments (PMNT-b-PEG-b-PMNT) with a polyanion, which formed a flower-type micelle via electrostatic complexation. Lidocaine could be stably incorporated in its core. When the temperature of the solution was increased to 37°C, the PIC-type flower micelle transformed to gel. The continuous release of lidocaine from the gel was observed for more than three days, without remarkable initial burst, which is probably owing to the stable entrapment of lidocaine in the PIC core of the gel. We evaluated the analgesic effect of RIG in carrageenan-induced arthritis mouse model. Results showed that lidocaine-loaded RIG has stronger and longer analgesic effect when administered in inflamed areas. In contrast, while the use of non-complexed lidocaine did not show analgesic effect one day after its administration. Note that no effect was observed when PIC-type flower micelle without ROS-scavenging ability was used. These findings suggest that local anesthetic-loaded RIG can effectively reduce the number of injection times and limit the side effects associated with the use of anti-inflammatory drugs for postoperative pain management. 1. We have been working on nanomaterials, which effectively eliminate ROS, avoiding dysfunction of mitochondria in healthy cells. 2. We designed redox injectable gel using polyion complexed flower type micelle, which can eliminates ROS locally. 3. We could prepare local anesthesia-loaded redox injectable gel (lido@RIG). 4. Drug release could be extended by local administration of lido@RIG. 5. Deprotonation of lidocaine improved anesthetic effect because ROS were eliminated locally by RIG. 6. Local inflammation could be also suppressed by lido@RIG. Copyright © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Advantages and limitations of remotely operated sea floor drill rigs

NASA Astrophysics Data System (ADS)

Freudenthal, T.; Smith, D. J.; Wefer, G.

2009-04-01

A variety of research targets in marine sciences including the investigation of gas hydrates, slope stability, alteration of oceanic crust, ore formation and palaeoclimate can be addressed by shallow drilling. However, drill ships are mostly used for deep drillings, both because the effort of building up a drill string from a drill ship to the deep sea floor is tremendous and control on drill bit pressure from a movable platform and a vibrating drill string is poor especially in the upper hundred meters. During the last decade a variety of remotely operated drill rigs have been developed, that are deployed on the sea bed and operated from standard research vessels. These developments include the BMS (Bentic Multicoring System, developed by Williamson and Associates, operated by the Japanese Mining Agency), the PROD (Portable Remotely Operated Drill, developed and operated by Benthic Geotech), the Rockdrill 2 (developed and operated by the British geological Survey) and the MeBo (German abbreviation for sea floor drill rig, developed and operated by Marum, University of Bremen). These drill rigs reach drilling depths between 15 and 100 m. For shallow drillings remotely operated drill rigs are a cost effective alternative to the services of drill ships and have the major advantage that the drilling operations are performed from a stable platform independent of any ship movements due to waves, wind or currents. Sea floor drill rigs can be deployed both in shallow waters and the deep sea. A careful site survey is required before deploying the sea floor drill rig. Slope gradient, small scale topography and soil strength are important factors when planning the deployment. The choice of drill bits and core catcher depend on the expected geology. The required drill tools are stored on one or two magazines on the drill rig. The MeBo is the only remotely operated drill rig world wide that can use wire line coring technique. This method is much faster than conventional drilling. It has the advantage that the drill string stays in the drilled hole during the entire drilling process and prevents the drilled hole from collapsing while the inner core barrels comprising the drilled core sections are hooked up inside the drill string using a wire.

Chemseal 3808-A2 penetration into small leak path

NASA Technical Reports Server (NTRS)

Carruth, M. R., Jr.; Dehaye, R. F.

1988-01-01

A possible fix to a leak in the oxidizer system of the Space Shuttle Discovery's attitude control system was proposed by MSFC. This fix involved the passing of a shuttlecock past the leaking Dynaflow fitting and sealing the vent tube containing the fitting with Chemseal 3808-A2. The question of whether the Chemseal 3808-A2 can flow into the leak path and provide a better seal was addressed analytically and by experiment to verify the analytical formula used. The results show that the equations are applicable and that the Chemseal will flow into the expected leak path and seal.

Occupational Fatalities Resulting from Falls in the Oil and Gas Extraction Industry, United States, 2005-2014.

PubMed

Mason, Krystal L; Retzer, Kyla D; Hill, Ryan; Lincoln, Jennifer M

2017-04-28

During 2003-2013, fatality rates for oil and gas extraction workers decreased for all causes of death except those associated with fall events, which increased 2% annually during 2003-2013 (1). To better understand risk factors for these events, CDC examined fatal fall events in the oil and gas extraction industry during 2005-2014 using data from case investigations conducted by the Occupational Safety and Health Administration (OSHA). Sixty-three fatal falls were identified, accounting for 15% of all fatal events. Among fatal falls, 33 (52%) workers fell from a height of >30 feet (9 meters), and 22 (35%) fell from the derrick board, the elevated work platform located in the derrick (structure used to support machinery on a drilling rig). Fall fatalities occurred most frequently when drilling rigs were being assembled or disassembled at the well site (rigging up or rigging down) (14; 22%) or when workers were removing or inserting drill pipe into the wellbore (14; 22%). Measures that target derrickmen and workers engaged in assembling and disassembling drilling rigs (rigging up and down) could reduce falls in this industry. Companies should annually update their fall protection plans and ensure effective fall prevention programs are in place for workers at highest risk for falls, including providing trainings on proper use, fit, and inspection of personal protective equipment.

Cloning of a gene (RIG-G) associated with retinoic acid-induced differentiation of acute promyelocytic leukemia cells and representing a new member of a family of interferon-stimulated genes

PubMed Central

Yu, Man; Tong, Jian-Hua; Mao, Mao; Kan, Li-Xin; Liu, Meng-Min; Sun, Yi-Wu; Fu, Gang; Jing, Yong-Kui; Yu, Long; Lepaslier, Denis; Lanotte, Michel; Wang, Zhen-Yi; Chen, Zhu; Waxman, Samuel; Wang, Ya-Xin; Tan, Jia-Zhen; Chen, Sai-Juan

1997-01-01

In a cell line (NB4) derived from a patient with acute promyelocytic leukemia, all-trans-retinoic acid (ATRA) and interferon (IFN) induce the expression of a novel gene we call RIG-G (for retinoic acid-induced gene G). This gene codes for a 58-kDa protein containing 490 amino acids with several potential sites for post-translational modification. In untreated NB4 cells, the expression of RIG-G is undetectable. ATRA treatment induces the transcriptional expression of RIG-G relatively late (12–24 hr) in a protein synthesis-dependent manner, whereas IFN-α induces its expression early (30 min to 3 hr). Database search has revealed a high-level homology between RIG-G and several IFN-stimulated genes in human (ISG54K, ISG56K, and IFN-inducible and retinoic acid-inducible 58K gene) and some other species, defining a well conserved gene family. The gene is composed of two exons and has been mapped by fluorescence in situ hybridization to chromosome 10q24, where two other human IFN-stimulated gene members are localized. A synergistic induction of RIG-G expression in NB4 cells by combined treatment with ATRA and IFNs suggests that a collaboration exists between their respective signaling pathways. PMID:9207104

Design and Checkout of a High Speed Research Nozzle Evaluation Rig

NASA Technical Reports Server (NTRS)

Castner, Raymond S.; Wolter, John D.

1997-01-01

The High Flow Jet Exit Rig (HFJER) was designed to provide simulated mixed flow turbojet engine exhaust for one- seventh scale models of advanced High Speed Research test nozzles. The new rig was designed to be used at NASA Lewis Research Center in the Nozzle Acoustic Test Rig and the 8x6 Supersonic Wind Tunnel. Capabilities were also designed to collect nozzle thrust measurement, aerodynamic measurements, and acoustic measurements when installed at the Nozzle Acoustic Test Rig. Simulated engine exhaust can be supplied from a high pressure air source at 33 pounds of air per second at 530 degrees Rankine and nozzle pressure ratios of 4.0. In addition, a combustion unit was designed from a J-58 aircraft engine burner to provide 20 pounds of air per second at 2000 degrees Rankine, also at nozzle pressure ratios of 4.0. These airflow capacities were designed to test High Speed Research nozzles with exhaust areas from eighteen square inches to twenty-two square inches. Nozzle inlet flow measurement is available through pressure and temperature sensors installed in the rig. Research instrumentation on High Speed Research nozzles is available with a maximum of 200 individual pressure and 100 individual temperature measurements. Checkout testing was performed in May 1997 with a 22 square inch ASME long radius flow nozzle. Checkout test results will be summarized and compared to the stated design goals.

A sealable ultrathin window sample cell for the study of liquids by means of soft X-ray spectroscopy

NASA Astrophysics Data System (ADS)

Grötzsch, D.; Streeck, C.; Nietzold, C.; Malzer, W.; Mantouvalou, I.; Nutsch, A.; Dietrich, P.; Unger, W.; Beckhoff, B.; Kanngießer, B.

2017-12-01

A new sample cell concept for the analysis of liquids or solid-liquid interfaces using soft X-ray spectroscopy is presented, which enables the complete sealing of the cell as well as the transport into vacuum via, for example, a load-lock system. The cell uses pressure monitoring and active as well as passive pressure regulation systems, thereby facilitating the full control over the pressure during filling, sealing, evacuation, and measurement. The cell design and sample preparation as well as the crucial sealing procedure are explained in detail. As a first proof-of-principle experiment, successful nitrogen K-edge fluorescence yield near-edge X-ray absorption fine structure experiments of a biomolecular solution are presented. For this purpose, it is shown that the careful evaluation of all involved parameters, such as window type or photon flux, is desirable for optimizing the experimental result.

Mechanical Design of a Performance Test Rig for the Turbine Air-Flow Task (TAFT)

NASA Technical Reports Server (NTRS)

Xenofos, George; Forbes, John; Farrow, John; Williams, Robert; Tyler, Tom; Sargent, Scott; Moharos, Jozsef

2003-01-01

To support development of the Boeing-Rocketdyne RS84 rocket engine, a fill-flow, reaction turbine geometry was integrated into the NASA-MSFC turbine air-flow test facility. A mechanical design was generated which minimized the amount of new hardware while incorporating all test and instrUmentation requirements. This paper provides details of the mechanical design for this Turbine Air-Flow Task (TAFT) test rig. The mechanical design process utilized for this task included the following basic stages: Conceptual Design. Preliminary Design. Detailed Design. Baseline of Design (including Configuration Control and Drawing Revision). Fabrication. Assembly. During the design process, many lessons were learned that should benefit future test rig design projects. Of primary importance are well-defined requirements early in the design process, a thorough detailed design package, and effective communication with both the customer and the fabrication contractors. The test rig provided steady and unsteady pressure data necessary to validate the computational fluid dynamics (CFD) code. The rig also helped characterize the turbine blade loading conditions. Test and CFD analysis results are to be presented in another JANNAF paper.

Acoustic Characteristics of Various Treatment Panel Designs Specific to HSCT Mixer-Ejector Application

NASA Technical Reports Server (NTRS)

Salikuddin, M.; Kinzie, K.; Vu, D. D.; Langenbrunner, L. E.; Szczepkowski, G. T.

2006-01-01

The development process of liner design methodology is described in several reports. The results of the initial effort of concept development, screening, laboratory testing of various liner concepts, and preliminary correlation (generic data) are presented in a report Acoustic Characteristics of Various Treatment Panel Designs for HSCT Ejector Liner Acoustic Technology Development Program. The second phase of laboratory test results of more practical concepts and their data correlations are presented in this report (product specific). In particular, this report contains normal incidence impedance measurements of several liner types in both a static rig and in a high temperature flow duct rig. The flow duct rig allows for temperatures up to 400 F with a grazing flow up to Mach 0.8. Measurements of impedance, DC flow resistance, and in the flow rig cases, impact of the liner on boundary layer profiles are documented. In addition to liner rig tests, a limited number of tests were made on liners installed in a mixer-Ejector nozzle to confirm the performance of the liner prediction in an installed configuration.

Design and Implementation of a Characterization Test Rig for Evaluating High Bandwidth Liquid Fuel Flow Modulators

NASA Technical Reports Server (NTRS)

Saus, Joseph R.; Chang, Clarence T.; DeLaat, John C.; Vrnak, Daniel R.

2010-01-01

A test rig was designed and developed at the NASA Glenn Research Center (GRC) for the purpose of characterizing high bandwidth liquid fuel flow modulator candidates to determine their suitability for combustion instability control research. The test rig is capable of testing flow modulators at up to 600 psia supply pressure and flows of up to 2 gpm. The rig is designed to provide a quiescent flow into the test section in order to isolate the dynamic flow modulations produced by the test article. Both the fuel injector orifice downstream of the test article and the combustor are emulated. The effect of fuel delivery line lengths on modulator dynamic performance can be observed and modified to replicate actual fuel delivery systems. For simplicity, water is currently used as the working fluid, although future plans are to use jet fuel. The rig is instrumented for dynamic pressures and flows and a high-speed data system is used for dynamic data acquisition. Preliminary results have been obtained for one candidate flow modulator.

[Water-cooled laser sealing of lung tissue in an ex-vivo ventilated porcine lung model].

PubMed

Tonoyan, T; Prisadov, G; Menges, P; Herrmann, K; Bobrov, P; Linder, A

2014-06-01

Laser resections of lung metastases are followed by air leaks from the parenchymal defect. Large surfaces after metastasectomy are closed by sutures or sealants while smaller areas are frequently sealed thermally by cautery or laser. In this study two different techniques of thermal sealing of lung tissue with laser light are investigated. Carbonisation of lung tissue during thermal sealing appears at temperatures higher than 180 °C. Hypothetically this is contraproductive to haemo- as well as to pneumostasis. In this experimental study thermal laser sealing with and without carbonisation is investigated. In one series tissue temperatures higher than 100 °C are avoided by water dropping from the tip of the light guide onto the parenchymal leak. In the other series carbonisation appeared because the laser light was applied in the non-contact mode without tissue cooling. The characteristics of the laser were 40 W, 1350 nm continuous mode. Air leaks (Vt) were measured with a simple and fast technique with high precision. The sealing effect of either series was defined as S = (1-Vt/V0) and the difference of S was statistically examined. The basic values V0 before sealing were about the same in both series. The air leaks Vt after 15, 30 and 45 s of sealing varied significantly in both series (p = 0.03). During simultaneous cooling the sealing effect was increasing with the duration of laser application, while it became worse in the series without cooling. Histological examination of the sealing zone showed only coagulation of the tissue, while ruptured alveolae could be seen more often in the non-cooled sealing area. It could be shown in the ex-vivo lung model that laser sealing of parenchymal leaks is improved by simultaneous cooling during laser application. Non cooled laser sealing seems to heat up the tissue abruptly and create carbonisation followed by multiple ruptures of alveola and small airways. In accordance with our clinical experience this experimental study confirms that laser sealing for pneumostasis after metastasectomy can be improved by simultaneously cooling the resection area when treated with the laser. Georg Thieme Verlag KG Stuttgart · New York.

Experience gained from using water and steam for bringing the operation of aircraft- and marine-derivative gas-turbine engines in compliance with environmental standards

NASA Astrophysics Data System (ADS)

Datsenko, V. V.; Zeigarnik, Yu. A.; Kosoi, A. S.

2014-04-01

Practical experience gained from using water and steam admission into the combustion chambers of aircraft- and marine-derivative gas turbines for bringing their operation in compliance with the requirements of environmental standards is described. The design and schematic modifications of combustion chambers and fuel system through which this goal is achieved are considered. The results obtained from industrial and rig tests of combustion chambers fitted with water or steam admission systems are presented.

Cryogenic gear technology for an orbital transfer vehicle engine and tester design

NASA Technical Reports Server (NTRS)

Calandra, M.; Duncan, G.

1986-01-01

Technology available for gears used in advanced Orbital Transfer Vehicle rocket engines and the design of a cryogenic adapted tester used for evaluating advanced gears are presented. The only high-speed, unlubricated gears currently in cryogenic service are used in the RL10 rocket engine turbomachinery. Advanced rocket engine gear systems experience operational load conditions and rotational speed that are beyond current experience levels. The work under this task consisted of a technology assessment and requirements definition followed by design of a self-contained portable cryogenic adapted gear test rig system.

Experiments on reduction of propeller induced interior noise by active control of cylinder vibration

NASA Technical Reports Server (NTRS)

Fuller, C. R.; Jones, J. D.

1987-01-01

The feasibility of reducing interior noise caused by advanced turbo propellers by controlling the vibration of aircraft fuselages was investigated by performing experiments in an anechoic chamber with an aircraft model test rig and apparatus. It was found that active vibration control provides reasonable global attenuation of interior noise levels for the cases of resonant (at 576 Hz) and forced (at 708 Hz) system response. The controlling mechanism behind the effect is structural-acoustic coupling between the shell and the contained field, termed interface modal filtering.

Engagement of Metal Debris into a Gear Mesh

NASA Technical Reports Server (NTRS)

Handschuh, Robert F.; Krantz, Timothy L.

2010-01-01

A series of bench top experiments was conducted to determine the effects of metallic debris being dragged through meshing gear teeth. A test rig that is typically used to conduct contact fatigue experiments was used for these tests. Several sizes of drill material, shim stock, and pieces of gear teeth were introduced and then driven through the meshing region. The level of torque required to drive the "chip" through the gear mesh was measured. From the data gathered, chip size sufficient to jam the mechanism can be determined.

Efficiency of a borehole seal by means of pre-compacted bentonite blocks

NASA Astrophysics Data System (ADS)

Van Geet, M.; Volckaert, G.; Bastiaens, W.; Maes, N.; Weetjens, E.; Sillen, X.; Vallejan, B.; Gens, A.

The backfilling and sealing of shafts and galleries is an essential part of the design of underground repositories for high-level radioactive waste. Part of the EC funded project RESEAL studied the feasibility of sealing off a borehole in plastic Boom Clay by means of pre-compacted bentonite blocks. Two bentonites, namely the FoCa and Serrata clay, have been used. Based on laboratory tests, the bentonite blocks had an initial dry density of about 1.8 g/cm 3 to obtain a swelling pressure of about 4.4 MPa, corresponding to the in situ lithostatic stress, at full saturation. The set-up was equipped with several sensors to follow-up the behaviour of the seal and the surrounding host rock during hydration. Full saturation was reached after five months and was mainly reached by natural hydration. Swelling pressure was lower than originally foreseen due to the slow reconsolidation of the host rock. Later on, the efficiency of the seal with respect to water, gas and radionuclide migration was tested. The in situ measured permeability of the seals was about 5 × 10 -13 m/s. A gas breakthrough experiment did not show any preferential gas migration through the seal. No evidences of a preferential pathway could be detected from 125I tracer test results.

Numerical Simulation of Flow in a Whirling Annular Seal and Comparison with Experiments

NASA Technical Reports Server (NTRS)

Athavale, M. M.; Hendricks, R. C.; Steinetz, B. M.

1995-01-01

The turbulent flow field in a simulated annular seal with a large clearance/radius ratio (0.015) and a whirling rotor was simulated using an advanced 3D CFD code SCISEAL. A circular whirl orbit with synchronous whirl was imposed on the rotor center. The flow field was rendered quasi-steady by making a transformation to a totaling frame. Standard k-epsilon model with wall functions was used to treat the turbulence. Experimentally measured values of flow parameters were used to specify the seal inlet and exit boundary conditions. The computed flow-field in terms of the velocity and pressure is compared with the experimental measurements inside the seal. The agreement between the numerical results and experimental data with correction is fair to good. The capability of current advanced CFD methodology to analyze this complex flow field is demonstrated. The methodology can also be extended to other whirl frequencies. Half- (or sub-) synchronous (fluid film unstable motion) and synchronous (rotor centrifugal force unbalance) whirls are the most unstable whirl modes in turbomachinery seals, and the flow code capability of simulating the flows in steady as well as whirling seals will prove to be extremely useful in the design, analyses, and performance predictions of annular as well as other types of seals.

A New Design of the Test Rig to Measure the Transmission Error of Automobile Gearbox

NASA Astrophysics Data System (ADS)

Hou, Yixuan; Zhou, Xiaoqin; He, Xiuzhi; Liu, Zufei; Liu, Qiang

2017-12-01

Noise and vibration affect the performance of automobile gearbox. And transmission error has been regarded as an important excitation source in gear system. Most of current research is focused on the measurement and analysis of single gear drive, and few investigations on the transmission error measurement in complete gearbox were conducted. In order to measure transmission error in a complete automobile gearbox, a kind of electrically closed test rig is developed. Based on the principle of modular design, the test rig can be used to test different types of gearbox by adding necessary modules. The test rig for front engine, rear-wheel-drive gearbox is constructed. And static and modal analysis methods are taken to verify the performance of a key component.

Mach 0.3 Burner Rig Facility at the NASA Glenn Materials Research Laboratory

NASA Technical Reports Server (NTRS)

Fox, Dennis S.; Miller, Robert A.; Zhu, Dongming; Perez, Michael; Cuy, Michael D.; Robinson, R. Craig

2011-01-01

This Technical Memorandum presents the current capabilities of the state-of-the-art Mach 0.3 Burner Rig Facility. It is used for materials research including oxidation, corrosion, erosion and impact. Consisting of seven computer controlled jet-fueled combustors in individual test cells, these relatively small rigs burn just 2 to 3 gal of jet fuel per hour. The rigs are used as an efficient means of subjecting potential aircraft engine/airframe advanced materials to the high temperatures, high velocities and thermal cycling closely approximating actual operating environments. Materials of various geometries and compositions can be evaluated at temperatures from 700 to 2400 F. Tests are conducted not only on bare superalloys and ceramics, but also to study the behavior and durability of protective coatings applied to those materials.

A New Foil Air Bearing Test Rig for Use to 700 C and 70,000 rpm

NASA Technical Reports Server (NTRS)

DellaCorte, Chris

1997-01-01

A new test rig has been developed for evaluating foil air bearings at high temperatures and speeds. These bearings are self acting hydrodynamic air bearings which have been successfully applied to a variety of turbomachinery operating up to 650 C. This unique test rig is capable of measuring bearing torque during start-up, shut-down and high speed operation. Load capacity and general performance characteristics, such as durability, can be measured at temperatures to 700 C and speeds to 70,000 rpm. This paper describes the new test rig and demonstrates its capabilities through the preliminary characterization of several bearings. The bearing performance data from this facility can be used to develop advanced turbomachinery incorporating high temperature oil-free air bearing technology.

Coping with Physiological Oxidative Stress: A Review of Antioxidant Strategies in Seals

PubMed Central

Vázquez-Medina, José Pablo; Zenteno-Savín, Tania; Elsner, Robert; Ortiz, Rudy M.

2012-01-01

While diving, seals are exposed to apnea-induced hypoxemia and repetitive cycles of ischemia/reperfusion. While on land, seals experience sleep apnea, as well as prolonged periods of food and water deprivation. Prolonged fasting, sleep apnea, hypoxemia and ischemia/reperfusion increase oxidant production and oxidative stress in terrestrial mammals. In seals, however, neither prolonged fasting nor apnea-induced hypoxemia or ischemia/reperfusion increase systemic or local oxidative damage. The strategies seals evolved to cope with increased oxidant production are reviewed in the present manuscript. Among these strategies, high antioxidant capacity and the oxidant-mediated activation of hormetic responses against hypoxia and oxidative stress are discussed. In addition to expanding our knowledge of the evolution of antioxidant defenses and adaptive responses to oxidative stress, understanding the mechanisms that allow adapted mammals to avoid oxidative damage has the potential to advance our knowledge of oxidative stress-induced pathologies and to enhance the translative value of biomedical therapies in the long term. PMID:22327141

The Eco-Hydrological Role of Physical Surface Sealing in Dry Environments

NASA Astrophysics Data System (ADS)

Sela, Shai; Svoray, Tal; Assouline, Shmuel

2016-04-01

Soil surface sealing is a widespread natural process in dry environments occurring frequently in bare soil areas between vegetation patches. The low hydraulic conductivity that characterizes the seal layer reduces both infiltration and evaporation fluxes from the soil, and thus has the potential to affect local vegetation water availability and consequently transpiration rates. This effect is investigated here using two separate physically based models - a runoff model, and a root water uptake model. High resolution rainfall data is used to demonstrate the seal layer effect on runoff generation and vegetation water availability, while the seal layer effect on vegetation water uptake is studied using a long-term climatic dataset (44 years) from three dry sites presenting a climatic gradient in the Negev Desert, Israel. The Feddes water uptake parameters for the dominant shrub at the study site (Sarcopoterium spinosum) were acquired using an inverse calibration procedure using data from a lysimeter experiment. The results indicate that the presence of surface sealing increases significantly vegetation water availability through runoff generation. Following water infiltration, the shrub transpiration generally increases if the shrub is surrounded by a seal layer, but this effect can switch from positive to negative depending on initial soil water content, rainfall intensity, and the duration of the subsequent drying intervals. These factors have a marked effect on inter-annual variability of the seal layer effect on the shrub transpiration, which on average was found to be 26% higher under sealed conditions than in the case of unsealed soil surfaces. These results shed light on the importance of surface sealing on the eco-hydrology of dry environments and its contribution to the resilience of woody vegetation.

The effect of venting on cookoff of a melt-castable explosive (Comp-B)

DOE PAGES

Hobbs, Michael L.; Kaneshige, Michael J.

2015-03-01

Occasionally, our well-controlled cookoff experiments with Comp-B give anomalous results when venting conditions are changed. For example, a vented experiment may take longer to ignite than a sealed experiment. In the current work, we show the effect of venting on thermal ignition of Comp-B. We use Sandia’s Instrumented Thermal Ignition (SITI) experiment with various headspace volumes in both vented and sealed geometries to study ignition of Comp-B. In some of these experiments, we have used a boroscope to observe Comp-B as it melts and reacts. We propose that the mechanism for ignition involves TNT melting, dissolution of RDX, and complexmore » bubbly liquid flow. High pressure inhibits bubble formation and flow is significantly reduced. At low pressure, a vigorous dispersed bubble flow was observed.« less

Carbon dioxide sequestration induced mineral precipitation healing of fractured reservoir seals

NASA Astrophysics Data System (ADS)

Welch, N.; Crawshaw, J.

2017-12-01

Initial experiments and the thermodynaic basis for carbon dioxide sequestration induced mineral precipitation healing of fractures through reservoir seals will be presented. The basis of this work is the potential exists for the dissolution of reservoir host rock formation carbonate minerals in the acidified injection front of CO2 during sequestration or EOR. This enriched brine and the bulk CO2 phase will then flow through the reservoir until contact with the reservoir seal. At this point any fractures present in the reservoir seal will be the preferential flow path for the bulk CO2 phase as well as the acidified brine front. These fractures would currently be filled with non-acidified brine saturated in seal formation brine. When the acidifeid brine from the host formation and the cap rock brine mix there is the potential for minerals to fall out of solution, and for these precipitated minerals to decrease or entirely cut off the fluid flow through the fractures present in a reservoir seal. Initial equilibrium simulations performed using the PHREEQC1 database drived from the PHREEQE2 database are used to show the favorable conditions under which this mineral precipitation can occurs. Bench scale fluid mixing experiments were then performed to determine the kinetics of the mineral precipitation process, and determine the progress of future experiemnts involving fluid flow within fractured anhydrite reservoir seal samples. 1Parkhurst, D.L., and Appelo, C.A.J., 2013, Description of input and examples for PHREEQC version 3—A computer program for speciation, batch-reaction, one-dimensional transport, and inverse geochemical calculations: U.S. Geological Survey Techniques and Methods, book 6, chap. A43, 497 p., available only at https://pubs.usgs.gov/tm/06/a43/. 2Parkhurst, David L., Donald C. Thorstenson, and L. Niel Plummer. PHREEQE: a computer program for geochemical calculations. No. 80-96. US Geological Survey, Water Resources Division,, 1980.

78 FR 70326 - Rigging Equipment for Material Handling; Extension of the Office of Management and Budget's (OMB...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-25

...OSHA solicits public comments concerning its proposal to extend the OMB approval of the information collection requirements contained in paragraphs (b)(1), (b)(6)(i), (b)(6)(ii), (c)(15)(ii), (e)(1)(i), (ii), and (iii) and (f)(2) of the Standard on Rigging Equipment for Material Handling (29 CFR 1926.251). These paragraphs require affixing identification tags or markings on rigging equipment, developing and maintaining inspection records, and retaining proof- testing certificates.

Species-Specific Inhibition of RIG-I Ubiquitination and IFN Induction by the Influenza A Virus NS1 Protein

PubMed Central

Rajsbaum, Ricardo; Albrecht, Randy A.; Wang, May K.; Maharaj, Natalya P.; Versteeg, Gijs A.; Nistal-Villán, Estanislao; García-Sastre, Adolfo; Gack, Michaela U.

2012-01-01

Influenza A viruses can adapt to new host species, leading to the emergence of novel pathogenic strains. There is evidence that highly pathogenic viruses encode for non-structural 1 (NS1) proteins that are more efficient in suppressing the host immune response. The NS1 protein inhibits type-I interferon (IFN) production partly by blocking the TRIM25 ubiquitin E3 ligase-mediated Lys63-linked ubiquitination of the viral RNA sensor RIG-I, required for its optimal downstream signaling. In order to understand possible mechanisms of viral adaptation and host tropism, we examined the ability of NS1 encoded by human (Cal04), avian (HK156), swine (SwTx98) and mouse-adapted (PR8) influenza viruses to interact with TRIM25 orthologues from mammalian and avian species. Using co-immunoprecipitation assays we show that human TRIM25 binds to all tested NS1 proteins, whereas the chicken TRIM25 ortholog binds preferentially to the NS1 from the avian virus. Strikingly, none of the NS1 proteins were able to bind mouse TRIM25. Since NS1 can inhibit IFN production in mouse, we tested the impact of TRIM25 and NS1 on RIG-I ubiquitination in mouse cells. While NS1 efficiently suppressed human TRIM25-dependent ubiquitination of RIG-I 2CARD, NS1 inhibited the ubiquitination of full-length mouse RIG-I in a mouse TRIM25-independent manner. Therefore, we tested if the ubiquitin E3 ligase Riplet, which has also been shown to ubiquitinate RIG-I, interacts with NS1. We found that NS1 binds mouse Riplet and inhibits its activity to induce IFN-β in murine cells. Furthermore, NS1 proteins of human but not swine or avian viruses were able to interact with human Riplet, thereby suppressing RIG-I ubiquitination. In conclusion, our results indicate that influenza NS1 protein targets TRIM25 and Riplet ubiquitin E3 ligases in a species-specific manner for the inhibition of RIG-I ubiquitination and antiviral IFN production. PMID:23209422

Species-specific inhibition of RIG-I ubiquitination and IFN induction by the influenza A virus NS1 protein.

PubMed

Rajsbaum, Ricardo; Albrecht, Randy A; Wang, May K; Maharaj, Natalya P; Versteeg, Gijs A; Nistal-Villán, Estanislao; García-Sastre, Adolfo; Gack, Michaela U

2012-01-01

Influenza A viruses can adapt to new host species, leading to the emergence of novel pathogenic strains. There is evidence that highly pathogenic viruses encode for non-structural 1 (NS1) proteins that are more efficient in suppressing the host immune response. The NS1 protein inhibits type-I interferon (IFN) production partly by blocking the TRIM25 ubiquitin E3 ligase-mediated Lys63-linked ubiquitination of the viral RNA sensor RIG-I, required for its optimal downstream signaling. In order to understand possible mechanisms of viral adaptation and host tropism, we examined the ability of NS1 encoded by human (Cal04), avian (HK156), swine (SwTx98) and mouse-adapted (PR8) influenza viruses to interact with TRIM25 orthologues from mammalian and avian species. Using co-immunoprecipitation assays we show that human TRIM25 binds to all tested NS1 proteins, whereas the chicken TRIM25 ortholog binds preferentially to the NS1 from the avian virus. Strikingly, none of the NS1 proteins were able to bind mouse TRIM25. Since NS1 can inhibit IFN production in mouse, we tested the impact of TRIM25 and NS1 on RIG-I ubiquitination in mouse cells. While NS1 efficiently suppressed human TRIM25-dependent ubiquitination of RIG-I 2CARD, NS1 inhibited the ubiquitination of full-length mouse RIG-I in a mouse TRIM25-independent manner. Therefore, we tested if the ubiquitin E3 ligase Riplet, which has also been shown to ubiquitinate RIG-I, interacts with NS1. We found that NS1 binds mouse Riplet and inhibits its activity to induce IFN-β in murine cells. Furthermore, NS1 proteins of human but not swine or avian viruses were able to interact with human Riplet, thereby suppressing RIG-I ubiquitination. In conclusion, our results indicate that influenza NS1 protein targets TRIM25 and Riplet ubiquitin E3 ligases in a species-specific manner for the inhibition of RIG-I ubiquitination and antiviral IFN production.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reynolds, Richard Jennings

The purpose of this course is to introduce safe hoisting and rigging practices to personnel who are attempting to become LANL incidental crane operators and to review and refresh safe hoisting and rigging practices with existing incidental crane operators.

29 CFR 1926.753 - Hoisting and rigging.

Code of Federal Regulations, 2012 CFR

2012-07-01

... devices, anti-two block devices, and load moment indicators where required; (D) Air, hydraulic, and other... members: (i) Attached at their center of gravity and maintained reasonably level; (ii) Rigged from top...

29 CFR 1926.753 - Hoisting and rigging.

Code of Federal Regulations, 2013 CFR

2013-07-01

... devices, anti-two block devices, and load moment indicators where required; (D) Air, hydraulic, and other... members: (i) Attached at their center of gravity and maintained reasonably level; (ii) Rigged from top...

29 CFR 1926.753 - Hoisting and rigging.

Code of Federal Regulations, 2014 CFR

2014-07-01

... devices, anti-two block devices, and load moment indicators where required; (D) Air, hydraulic, and other... members: (i) Attached at their center of gravity and maintained reasonably level; (ii) Rigged from top...

An air bearing system for small high speed gas turbines

NASA Astrophysics Data System (ADS)

Turner, A. B.; Davies, S. J.; Nimir, Y. L.

1994-03-01

This paper describes the second phase of an experimental program concerning the application of air bearings to small turbomachinery test rigs and small gas turbines. The first phase examined externally pressurized (EP) journal bearings, with a novel EP thrust bearing, for application to 'warm air' test rigs, and was entirely successful at rotational speeds in excess of 100,000 rpm. This second phase examined several designs of tilting pad-spiring journal bearings, one with a novel form of externally pressurized pad, but all using the original EP thrust bearing. The designs tested are described, including some oscillogram traces, for tests up to a maximum of 70,000 rpm; the most successful using a carbon pad-titanium beam spring arrangement. The thrust bearing which gave trouble-free operation throughout, is also described. The results of an original experiment to measure the 'runway speed' of a radial inflow turbine are also presented, which show that overspeeds of 58 percent above the design speed can result from free-power turbine coupling failure.

Isothermal thermogravimetric data acquisition analysis system

NASA Technical Reports Server (NTRS)

Cooper, Kenneth, Jr.

1991-01-01

The description of an Isothermal Thermogravimetric Analysis (TGA) Data Acquisition System is presented. The system consists of software and hardware to perform a wide variety of TGA experiments. The software is written in ANSI C using Borland's Turbo C++. The hardware consists of a 486/25 MHz machine with a Capital Equipment Corp. IEEE488 interface card. The interface is to a Hewlett Packard 3497A data acquisition system using two analog input cards and a digital actuator card. The system provides for 16 TGA rigs with weight and temperature measurements from each rig. Data collection is conducted in three phases. Acquisition is done at a rapid rate during initial startup, at a slower rate during extended data collection periods, and finally at a fast rate during shutdown. Parameters controlling the rate and duration of each phase are user programmable. Furnace control (raising and lowering) is also programmable. Provision is made for automatic restart in the event of power failure or other abnormal terminations. Initial trial runs were conducted to show system stability.

Acoustic Characterization of Compact Jet Engine Simulator Units

NASA Technical Reports Server (NTRS)

Doty, Michael J.; Haskin, Henry H.

2013-01-01

Two dual-stream, heated jet, Compact Jet Engine Simulator (CJES) units are designed for wind tunnel acoustic experiments involving a Hybrid Wing Body (HWB) vehicle. The newly fabricated CJES units are characterized with a series of acoustic and flowfield investigations to ensure successful operation with minimal rig noise. To limit simulator size, consistent with a 5.8% HWB model, the CJES units adapt Ultra Compact Combustor (UCC) technology developed at the Air Force Research Laboratory. Stable and controllable operation of the combustor is demonstrated using passive swirl air injection and backpressuring of the combustion chamber. Combustion instability tones are eliminated using nonuniform flow conditioners in conjunction with upstream screens. Through proper flow conditioning, rig noise is reduced by more than 20 dB over a broad spectral range, but it is not completely eliminated at high frequencies. The low-noise chevron nozzle concept designed for the HWB test shows expected acoustic benefits when installed on the CJES unit, and consistency between CJES units is shown to be within 0.5 dB OASPL.

Effects of geometric variables on rub characteristics of Ti-6Al-4V

NASA Technical Reports Server (NTRS)

Bill, R. C.; Wolak, J.; Wisander, D. W.

1981-01-01

Experiments simulating rub interactions between Ti-6Al-4V blade tips and various seal materials were conducted. The number of blade tips and the blade tip geometry were varied to determine their effects on rub forces and on wear phenomena. Contact was found to be quite unsteady for all blade tip geometries except for those incorporating deliberately rounded blade tips. The unsteady contact was characterized by long periods of rubbing contact and increasing blade tip that terminated in sudden rapid metal removal, sometimes accompanied by tearing and disruption of porous seal material under the rub surface. A model describing the blade tip loading is proposed and is based on the propagation of an elastic stress wave through the seal material as the seal material is dynamically compressed by the blade tip leading edge.

A new leakage measurement method for damaged seal material

NASA Astrophysics Data System (ADS)

Wang, Shen; Yao, Xue Feng; Yang, Heng; Yuan, Li; Dong, Yi Feng

2018-07-01

In this paper, a new leakage measurement method based on the temperature field and temperature gradient field is proposed for detecting the leakage location and measuring the leakage rate in damaged seal material. First, a heat transfer leakage model is established, which can calculate the leakage rate based on the temperature gradient field near the damaged zone. Second, a finite element model of an infinite plate with a damaged zone is built to calculate the leakage rate, which fits the simulated leakage rate well. Finally, specimens in a tubular rubber seal with different damage shapes are used to conduct the leakage experiment, validating the correctness of this new measurement principle for the leakage rate and the leakage position. The results indicate the feasibility of the leakage measurement method for damaged seal material based on the temperature gradient field from infrared thermography.

Volatile organic compound and semivolatile organic compound outgassing rates for ethylene propylene diene monomer and fluoropolymer seals

NASA Astrophysics Data System (ADS)

Pecault, Isabelle Tovena

2017-11-01

High-power laser facilities, such as Laser MegaJoule, are currently being operated for inertial confinement fusion experiments. Emission of volatile organic compounds (VOC) and moreover semivolatile organic compounds (SVOCs) from seals in laser environment is of tremendous importance for the optics lifetime and laser performance. That is why all the seals were screening in the same conditions: 48 h at 30°C and three successive cycle of 1.5 h at 50°C. This paper focuses on the qualification test performed on three seals: two ethylene propylene diene monomer (EPDM) and one fluoropolymer (FPM). It is shown that the molded and the extruded EPDM do not outgas the same amount neither the same molecules whereas EPDM and FPM outgas nearly the same level of phthalates.

19. DECK VIEW LOOKING FORWARD WITH MAST, RIGGING AND BOWSPRIT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. DECK VIEW LOOKING FORWARD WITH MAST, RIGGING AND BOWSPRIT DETAILS - HATCH COVER REMOVED TO SHOW CENTERBOARD TRUNK - KATHRYN-Two-sail Bateau "Skipjack", Dogwood Harbor, Chesapeake Bay, Tilghman, Talbot County, MD

14. FACILITY IDENTIFICATION STENCILED ON ROOF BEAM, 'RIGGING LOFT' PORTION ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. FACILITY IDENTIFICATION STENCILED ON ROOF BEAM, 'RIGGING LOFT' PORTION OF BUILDING 4. - Chollas Heights Naval Radio Transmitting Facility, Public Works Shop, 6410 Zero Road, San Diego, San Diego County, CA

46 CFR 115.113 - Passengers permitted.

Code of Federal Regulations, 2010 CFR

2010-10-01

... sail booms, running rigging, or paddle wheels, or along pulpits; (ii) Rail space on stairways; and (iii... equipment, anchor handling equipment or line handling gear, or in the way of sail booms or running rigging...

46 CFR 176.113 - Passengers permitted.

Code of Federal Regulations, 2011 CFR

2011-10-01

... or line handling gear, in the way of sail booms, running rigging, or paddle wheels, or along pulpits... of sail booms or running rigging; (v) Spaces below deck that are unsuitable for passengers or that...

46 CFR 176.113 - Passengers permitted.

Code of Federal Regulations, 2010 CFR

2010-10-01

... or line handling gear, in the way of sail booms, running rigging, or paddle wheels, or along pulpits... of sail booms or running rigging; (v) Spaces below deck that are unsuitable for passengers or that...

46 CFR 115.113 - Passengers permitted.

Code of Federal Regulations, 2011 CFR

2011-10-01

... sail booms, running rigging, or paddle wheels, or along pulpits; (ii) Rail space on stairways; and (iii... equipment, anchor handling equipment or line handling gear, or in the way of sail booms or running rigging...

Advanced jack up rig breaking U.S. construction drought

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kelly, P.

1997-03-10

A new heavy duty jack up, due in mid-1998, will be able to simultaneously drill and produce wells in harsher environments and deeper water than current jack ups in the worldwide fleet. Rowan Cos. Inc.`s Gorilla V is the only mobile offshore drilling unit (MODU) currently under construction in the US. Two more enhanced Gorilla design rigs are planned before the year 2000. The enhanced Gorilla class jack up represents the most technologically advanced jack up unit constructed to date. The rigs are structurally designed to meet year-round weather challenges in the harshest geographical environments. Rising demand for drilling rigs,more » coupled with a dwindling fleet, is generating supply shortages around the world, particularly at the high-specification end of the market. Even increasing the historical retirement age from 20 to 25 years, rig attrition continues at a level of about 18 rigs per year. Apart from the jack up market per se, however, Rowan`s strategy in designing and building enhanced Gorillas is to improve existing jack up drilling technology and offer the versatility to operate as a drilling unit, a mobile production unit, or both simultaneously in either open water locations or alongside existing platforms. The paper discusses the market for these heavy jack-ups, the use of one on the Cohasset project in Nova Scotia, the Gorilla V and enhanced Gorillas, geographical range of use, and MOPU economics.« less

Droplet Combustion in a Slow Convective Flow

NASA Technical Reports Server (NTRS)

Nayagam, V.; Hicks, M. C.; Kaib, N.; Ackerman, M.; Haggard, J. B., Jr.; Williams, F. A.

2001-01-01

The objective of the present flight experiment definition study is to investigate the effects of slow forced convective flows on the dynamics of isolated single droplet combustion and is designed to complement the quiescent, microgravity droplet combustion experiments (DCE-1 and DCE-2) of Williams and Dryer. The fuels selected for this study are the same as those of DCE, namely, a sooting alkane fuel (heptane) and a non-sooting alcohol (methanol), and imposed flow rates are chosen between 0 and 20 cm/s with varying ambient oxygen concentrations and pressures. Within this velocity range, both accelerating and decelerating flow effects will also be investigated. Two different approaches to generate the forced flow are currently under development in ground-based facilities; the first is a flow tunnel concept where the forced flow is imposed against a stationary droplet, and in the second a tethered droplet is translated at a specified velocity in a quiescent ambient medium. Depending upon the engineering feasibility a selection will be made between these two approaches so that the experiment can be accommodated in the Multiple Droplet Combustion Apparatus (MDCA) currently being designed for the International Space Station. Recently, we have finished designing and fabricating the experimental rigs using both the above mentioned concepts. The flow tunnel concept is implemented in a 2.2 second drop tower rig. Preliminary experiments have been carried out using heptane and methanol in air at atmospheric pressure. The translating droplet apparatus is scheduled to be tested in the 5 second drop facility in the near future. This report presents some of the experimental results obtained for heptane.

Experiments on planetary ices at UCL

NASA Astrophysics Data System (ADS)

Grindrod, P. M.; Fortes, A. D.; Wood, I. G.; Dobson, D.; Sammonds, P. R.; Stone-Drake, L.; Vocadlo, L.

2007-08-01

Using a suite of techniques and equipment, we conduct several different types of experiments on planetary ices at UCL. Samples are prepared in the Ice Physics Laboratory, which consists of a 5 chamber complex of inter-connected cold rooms, controllable from +30 to -30 deg C. Within this laboratory we have a functioning triaxial deformation cell operating at low temperature (down to -90 deg C) and high pressures (300 MPa), an Automatic Ice Fabric Analyser (AIFA) and a low-temperature microscope with CCD output. Polycrystalline samples, 40mm diameter by 100mm long, are compressed in the triaxial rig with a confining pressure; single crystal specimens are compressed in a separate uniaxial creep rig which operates at zero confining pressure for surface studies. A cold stage is also available for study of ice microstructural studies on our new Jeol JSM-6480LV SEM, which also allows tensile, compression and/or bending tests, with load ranges from less than 2N to 5000N. Finally, we also use a cold stage on a new PANalytical, X'pert PRO MPD, high resolution powder diffractometer to study the structure and phase behaviour of icy materials. Recent highlights of our work include: (1) derivation of a manufacturing process for methane clathrate at low temperatures, analysed in the X-Ray Diffraction Laboratory, for future rheological experiments, (2) analysed the growth behaviour of MS11, (3) refurbished and commenced calibration tests on the triaxial deformation cell using ice Ih, and (4) performed creep tests on gypsum and epsomite using the single crystal deformation cell. Further experiments will build on these preliminary results.

Enhancing the Effects of Teacher Attunement to Student Peer Group Affiliations on the School Social-Affective Context: Promotive Effects of the SEALS Intervention

ERIC Educational Resources Information Center

Hamm, Jill V.

2011-01-01

The purpose of this study was to examine teachers' attunement to student peer group affiliations as a factor in students' experiences of the school social-affect context. First, the author and her colleagues hypothesize that teacher attunement will be greater in intervention versus control schools following initial SEALS training. Second, they…

Working with "Social and Emotional Aspects of Learning" (SEAL): Associations with School Ethos, Pupil Social Experiences, Attendance, and Attainment

ERIC Educational Resources Information Center

Banerjee, Robin; Weare, Katherine; Farr, William

2014-01-01

A programme of resources and activities relating to "Social and Emotional Aspects of Learning" (SEAL) has been rolled out nationally to primary and secondary schools in the UK, but we know little about how variations in the implementation of this work relate to key indicators of school success. In the present study, a team of experienced…

A versatile system for biological and soil chemical tests on a planetary landing craft. II - Hardware development

NASA Technical Reports Server (NTRS)

Martin, J. P.; Kok, B.; Radmer, R.

1976-01-01

A system has been under development which is designed to seek remotely for clues to life in planetary soil samples. The basic approach is a set of experiments, all having a common sensor, a gas analysis mass spectrometer which monitors gas composition in the head spaces above sealed, temperature controlled soil samples. Versatility is obtained with up to three preloaded, sealed fluid injector capsules for each of eleven soil test cells. Tests results with an engineering model has demonstrated performance capability of subsystem components such as soil distribution, gas sampling valves, injector mechanisms, temperature control, and test cell seal.

Bandages and difficulty with bathing: introducing Seal-Tight.

PubMed

Lindsay, Ellie

2005-06-01

Patients with compression bandages experience difficulty with bathing due to the possibility that bandages may become wet and affect the wound. Bandage and dressing changes resulting from accidental wetting also cost the NHS considerable time and money. This product focus highlights the social and psychological impact on the patient when they are unable to bathe and offers a solution to the problem. Seal-Tight is a product that has been newly placed on the drug tariff, making it widely available to all patients who wear bandages (or plaster casts). Seal-Tight enables the patient to bathe, in some cases for the first time for months or even years.

Evaluation of mechanical losses in a linear motor pressure wave generator

NASA Astrophysics Data System (ADS)

Jacob, Subhash; Rangasamy, Karunanithi; Jonnalagadda, Kranthi Kumar; Chakkala, Damu; Achanur, Mallappa; Govindswamy, Jagadish; Gour, Abhay Singh

2012-06-01

A moving magnet linear motor compressor or pressure wave generator (PWG) of 2 cc swept volume with dual opposed piston configuration has been developed to operate miniature pulse tube coolers. Prelimnary experiments yielded only a no-load cold end temperature of 180 K. Auxiliary tests and the interpretation of detailed modeling of a PWG suggest that much of the PV power has been lost in the form of blow-by at piston seals due to large and non-optimum clearance seal gap between piston and cylinder. The results of experimental parameters simulated using Sage provide the optimum seal gap value for maximizing the delivered PV power.

Differences in Ostomy Pouch Seal Leakage Occurrences Between North American and European Residents.

PubMed

Fellows, Jane; Forest Lalande, Louise; Martins, Lina; Steen, Anne; Størling, Zenia M

The purpose of this study was to compare experiences and concerns about pouch seal leakage between persons with ostomies residing in North America (Canada and the United States) and Europe (United Kingdom, Netherlands, Sweden, Germany, Belgium, France, and Italy). Differences in reported pouch wear time and accessories used between the 2 groups were also examined. Secondary analysis of data from a cross-sectional study (Ostomy Life Study). Responses from persons residing in European countries (n = 1939) were compared with responses of 1387 individuals residing in North American countries. Persons with an ostomy completed a questionnaire that focused on 4 topics related to the daily use of an ostomy pouching system (pouch seal leakage, ballooning, appearance of pouching system such as color and size of the pouch and whether it is discrete under clothing, and coupling failure of 2-piece pouching systems). Pouch seal leakage was defined as stomal effluent seeping between the skin and the wafer of the ostomy pouching system. Statistical analysis was performed using a proportional odds model including various variable effects. Special attention was given to frequency of pouch seal leakage occurrences. All tests were 2-sided; P values ≤.05 were deemed statistically significant. Participants living in the North American countries indicated they were more likely to experience leakage from the ostomy (odds ratio = 2.610, 95% CI 2.187-3.115; P < .0001). Findings also indicated they were more likely to worry about pouch seal leakage than those in the European countries' data set (odds ratio = 2.722, 95% CI 2.283-3.246; P < .0001). Participants residing in the North American countries had significantly longer wear times than those participants in the European countries (P < .0001, χ test). The use of accessories was associated with a longer pouching system wear time. Study results suggest that participants from the North American countries indicated significantly more experience with and worries about leakage and longer wear time than the participants from the European population. Additional research is needed to determine the reasons for these differences.

Qualitative Maintenance Experience Handbook. P-3C/S-3A Supplement.

DTIC Science & Technology

1977-06-15

axle which automatically assists in disc alignment, a positive feature, in easing maintenance and preventing casual damage. Brake assemblies should...Reverse Of Removal Use brake tool to align brake discs . After Installation Actions: _ Bleed _ Rig _ Adjust X Service Lubricate - Boresight. _ Other...Hydraulic I Access Required: Test Equipment Required: Actions: 1. Check clearance of discs after brakes are put on. 2. Apply brakes . 8 ANALYST’S COMMENTS

A Dynamic Clamp on Every Rig

PubMed Central

2017-01-01

Abstract The dynamic clamp should be a standard part of every cellular electrophysiologist’s toolbox. That it is not, even 25 years after its introduction, comes down to three issues: money, the disruption that adding dynamic clamp to an existing electrophysiology rig entails, and the technical prowess required of experimenters. These have been valid and limiting issues in the past, but no longer. Technological advances associated with the so-called maker movement render them moot. We demonstrate this by implementing a fast (∼100 kHz) dynamic clamp system using an inexpensive microcontroller (Teensy 3.6). The overall cost of the system is less than USD$100, and assembling it requires no prior electronics experience. Modifying it—for example, to add Hodgkin–Huxley-style conductances—requires no prior programming experience. The system works together with existing electrophysiology data acquisition systems (for Macintosh, Windows, and Linux); it does not attempt to supplant them. Moreover, the process of assembling, modifying, and using the system constitutes a useful pedagogical exercise for students and researchers with no background but an interest in electronics and programming. We demonstrate the system’s utility by implementing conductances as fast as a transient sodium conductance and as complex as the Ornstein–Uhlenbeck conductances of the “point conductance” model of synaptic background activity. PMID:29085905

EVAHEART: an implantable centrifugal blood pump for long-term circulatory support.

PubMed

Yamazaki, Kenji; Kihara, Shinichiro; Akimoto, Takehide; Tagusari, Osamu; Kawai, Akihiko; Umezu, Mitsuo; Tomioka, Jun; Kormos, Robert L; Griffith, Bartley P; Kurosawa, Hiromi

2002-11-01

We developed "EVAHEART": a compact centrifugal blood pump system as an implantable left ventricular assist device for long-term circulatory support. The 55 x 64 mm pump is made from pure titanium, and weighs 370 g. The entire blood-contacting surface is covered with an anti-thrombogenic coating of diamond like carbon (DLC) or 2-methacryloyloxyethyl phosphorylcholine (MPC) to improve blood compatibility. Flows exceeding 12 L/min against 100 mmHg pressure at 2600 rpm was measured. A low-temperature mechanical seal with recirculating cooling system is used to seal the shaft. EVAHEART demonstrated an acceptably low hemolysis rate with normalized index of hemolysis of 0.005 +/- 0.002 g/100L. We evaluated the pump in long-term in-vivo experiments with seven calves. Via left thoracotomy, we conducted left ventricular apex-descending aorta bypass, placing the pump in the left thoracic cavity. Pump flow rates was maintained at 5-9 L/min, pump power consumption remained stable at 9-10 W in all cases, plasma free Hb levels were less than 15 mg/dl, and the seal system showed good seal capability throughout the experiments. The calves were sacrificed on schedule on postoperative day 200, 222, 142, 90, 151, 155, and 133. No thrombi formed on the blood contacting surface with either the DLC or MPC coating, and no major organ thromboembolisms occurred except for a few small renal infarcts. EVAHEART centrifugal blood pump demonstrated excellent performance in long-term in-vivo experiments.

Development and performance of self-sealed MRPC

NASA Astrophysics Data System (ADS)

Lyu, P.; Wang, Y.; Guo, B.; Han, D.; Li, Y.; Herrmann, N.; Deppner, I.; Simon, C.; Weidenkaff, P.; Jochen, F.; Loizeau, P.-A.; Laden Kis, M.

2017-03-01

The MRPC has been widely applied in the high-energy physics experiments in recent decades for its excellent timing performance and good efficiency. The Compressed Baryonic Matter (CBM) experiment at FAIR plans to build its TOF system based on the MRPCs, aiming at a good hadron identification. The CBM-TOF will work at a very high particle flux rate (~ 30 kHz/cm2) condition, and the long-time exposure to such high-rate ionizing radiation will lead to severe gas aging problem on the MRPC. In order to improve the gas interchange inside the MRPC, we designed a new structure of self-sealed MRPC. It has a much smaller gas volume, which may accelerate the exchange process between the fresh and the polluted gas. The preparation time before the MRPC could work is also largely shortened. A prototype of self-sealed MRPC was produced in Tsinghua University, and it was tested in the CERN SPS Nov 2015 beam test organized by the CBM-TOF group. It showed around 98% efficiency and 60 ps time resolution. Due to the rate limit of 10 kHz/cm2 in this beam test, we cannot make judgement on its performance under extremely high rate. Therefore further studies have to be performed under higher rate in order to study the capability of improving the gas exchange speed of this more flexible and operable sealed MRPC. This will allow that MRPC to be better suited for the high rate environment of TOF system while operating in the experiment.

Demonstration of various rotor instabilities (exhibit of Bently Rotor Dynamics Research Corporation Laborator rigs at symposium on instability in rotaing machinery)

NASA Technical Reports Server (NTRS)

Muszynska, A.

1985-01-01

The operation of rotor rigs used to demonstrate various instability phenomena occurring in rotating machines is described. The instability phenomena demonstrated included oil whirl/whip antiswirl, rub, loose rotating parts, water-lubricated bearing instabilities, and cracked shaft. The rotor rigs were also used to show corrective measures for preventing instabilities. Vibrational response data from the rigs were taken with modern, computerized instrumentation. The rotor nonsynchronous perturbation rig demonstrated modal identification techniques for rotor/bearing systems. Computer-aided data acquisition and presentation, using the dynamic stiffness method, makes it possible to identify rotor and bearing parameters for low modes. The shaft mode demonstrator presented the amplified modal shape line of the shaft excited by inertia forces of unbalance (synchronous perturbation). The first three bending modes of the shaft can be demonstrated. The user-friendly software, Orbits, presented a simulation of rotor precessional motion that is characteristic of various instability phenomena. The data presentation demonstration used data measured on a turbine driven compressor train as an example of how computer aided data acquisition and presentation assists in identifying rotating machine malfunctions.

A novel mechanism for regulation of the type I IFN response by herpesvirus deconjugases.

PubMed

Gupta, Soham; Ylä-Anttila, Päivi; Masucci, Maria G

2018-04-11

Upon infection, viral nucleic acids are recognized by germline-encoded pattern-recognition receptors (PRRs), and cytosolic retinoic acid-inducible gene I (RIG-I)-like helicases (RLHs) that initiate signaling pathways resulting in the production of type I IFN and pro-inflammatory cytokines. Binding of RIG-I to viral nucleic acids triggers the formation of the RIG-I signalosome where RIG-I is ubiquitinated by the TRIM25 ligase and, with the help of 14-3-3 scaffolds, further translocated to mitochondrial anti-viral signalling proteins (MAVS). Subsequent ubiquitination-mediated events trigger transcriptional activation of the effectors of innate immunity. We have found a new mechanism by which herpesviruses interfere with this signalling pathway to favour the establishment of latency and promote virus replication. The cysteine protease encoded in the conserved N-terminal domain of the herpesvirus large tegument protein binds to 14-3-3 proteins and forms a tri-molecular complex with TRIM25, promoting the activation and autoubiquitination of the ligase. RIG-I is recruited to the complex but its ubiquitination is drastically reduced, which effectively inactivates downstream signalling and blocks the type I IFN response.

Water droplet erosion of stainless steel steam turbine blades

NASA Astrophysics Data System (ADS)

Kirols, H. S.; Kevorkov, D.; Uihlein, A.; Medraj, M.

2017-08-01

Steam turbine blades are highly subjected to water droplet erosion (WDE) caused by high energy impingement of liquid water droplets. However, most of the published research on this wear phenomenon is performed on laboratory test rigs, instead of addressing WDE of actual steam turbine blades. In this work, the progression of erosion on the surface of ex-service low pressure steam turbine blades was investigated using scanning electron microscopy. The erosion appearance and mechanisms are compared with laboratory test rig results that are carried out using a rotating disk rig according to ASTM G73 standard. Initial and advanced erosion stages could be observed on the steam turbine blades. Similar to the WDE rig coupons, initial pits and cracks were preceded by blade surface roughening through the formation of asperities and depressions. In addition, it was also observed that the twist angle of the turbine blade around its diagonal, is an important parameter that influences its WDE. Twist angle has an effect on: impact angle, erosion appearance, impact speed, and the affected area. Furthermore, according to the current experimental results, multi-ray rig erosion test results are considered the closest simulation to the actual ex-service blade in terms of damage appearance.

Experimental Performance Evaluation of a High Speed Permanent Magnet Synchronous Motor and Drive for a Flywheel Application at Different Frequencies

NASA Technical Reports Server (NTRS)

Nagorny, Aleksandr S.; Jansen, Ralph H.; Kankam, M. David

2007-01-01

This paper presents the results of an experimental performance characterization study of a high speed, permanent magnet motor/generator (M/G) and drive applied to a flywheel module. Unlike the conventional electric machine the flywheel M/G is not a separated unit; its stator and rotor are integrated into a flywheel assembly. The M/G rotor is mounted on a flywheel rotor, which is magnetically levitated and sealed within a vacuum chamber during the operation. Thus, it is not possible to test the M/G using direct load measurements with a dynamometer and torque transducer. Accordingly, a new in-situ testing method had to be developed. The paper describes a new flywheel M/G and drive performance evaluation technique, which allows the estimation of the losses, efficiency and power quality of the flywheel high speed permanent magnet M/G, while working in vacuum, over wide frequency and torque ranges. This method does not require any hardware modification nor any special addition to the test rig. This new measurement technique is useful for high-speed applications, when applying an external load is technically difficult.

Hot isostatically pressed manufacture of high strength MERL 76 disk and seal shapes

NASA Technical Reports Server (NTRS)

Eng, R. D.; Evans, D. J.

1982-01-01

The feasibility of using MERL 76, an advanced high strength direct hot isostatic pressed powder metallurgy superalloy, as a full scale component in a high technology, long life, commercial turbine engine were demonstrated. The component was a JT9D first stage turbine disk. The JT9D disk rim temperature capability was increased by at least 22 C and the weight of JT9D high pressure turbine rotating components was reduced by at least 35 pounds by replacement of forged Superwaspaloy components with hot isostatic pressed (HIP) MERL 76 components. The process control plan and acceptance criteria for manufacture of MERL 76 HIP consolidated components were generated. Disk components were manufactured for spin/burst rig test, experimental engine tests, and design data generation, which established lower design properties including tensile, stress-rupture, 0.2% creep and notched (Kt = 2.5) low cycle fatigue properties, Sonntag, fatigue crack propagation, and low cycle fatigue crack threshold data. Direct HIP MERL 76, when compared to conventionally forged Superwaspaloy, is demonstrated to be superior in mechanical properties, increased rim temperature capability, reduced component weight, and reduced material cost by at least 30% based on 1980 costs.

Advanced Gas Turbine (AGT) Technology Project

NASA Technical Reports Server (NTRS)

1986-01-01

Engine testing, ceramic component fabrication and evaluation, component performance rig testing, and analytical studies comprised AGT 100 activities during the 1985 year. Ten experimental assemblies (builds) were evaluated using two engines. Accrued operating time was 120 hr of burning and 170 hr total, bringing cumulative total operating time to 395 hr, all devoid of major failures. Tests identified the generator seals as the primary working fluid leakage sources. Power transfer clutch operation was demonstrated. An alpha SiC gasifier rotor engine test resulted in blade tip failures. Recurring case vibration and shaft whip have limited gasifier shaft speeds to 84%. Ceramic components successfully engine tested now include the SiC scroll assembly, Si3N3 turbine rotor, combustor assembly, regenerator disk bulkhead, turbine vanes, piston rings, and couplings. A compressor shroud design change to reduce heat recirculation back to the inlet was executed. Ceramic components activity continues to focus on the development of state-of-the-art material strength characteristics in full-scale engine hardware. Fiber reinforced glass-ceramic composite turbine (inner) backplates were fabricated by Corning Glass Works. The BMAS/III material performed well in engine testing. Backplates of MAS material have not been engine tested.

77 FR 23117 - Rigging Equipment for Material Handling Construction Standard; Correction and Technical Amendment

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-18

...OSHA is correcting its sling standard for construction titled ``Rigging Equipment for Material Handling'' by removing the rated capacity tables and making minor, nonsubstantive revisions to the regulatory text.

Modified fabrication techniques lead to improved centrifugal blood pump performance.

PubMed

Pacella, J J; Goldstein, A H; Magovern, G J; Clark, R F

1994-01-01

The authors are developing an implantable centrifugal blood pump for short- and medium-term (1-6 months) left ventricular assist. They hypothesized that the application of result dependent modifications to this pump would lead to overall improved performance in long-term implantation studies. Essential requirements for pump operation, such as durability and resistance to clot formation, have been achieved through specialized fabrication techniques. The antithrombogenic character of the pump has been improved through coating at the cannula-housing interfaces and the baffle seal, and through changing the impeller blade material from polysulfone to pyrolytic carbon. The electronic components of the pump have been sealed for implantable use through specialized processes of dipping and potting, and the surfaces of the internal pump components have been treated to increase durability. The device has demonstrated efficacy in five chronic sheep implantation studies of 14, 10, 28, 35, and 154 day duration. Post mortem findings from the 14 day experiment showed stable fibrin entangled around the impeller shaft and blades. After pump modification, autopsy findings of the 10 day study showed no evidence of clot. Additionally, the results of the 28 day experiment showed only a small (2.0 mm) ring of fibrin at the shaft-seal interface. In the 35 and 154 day experiments, redesign of the stators have resulted in improved motor corrosion resistance. The 35 day study showed a small, 0.5 mm wide fibrin deposit at the lip seal, but no motor failure. In the 154 day experiment, the motor failed because of stator fluid corrosion, while the explanted pump was devoid of thrombus. Based on these findings, the authors believe that these pump refinements have contributed significantly to improvements in durability and resistance to clot formation.

Welding of hermetic connectors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hieber, D.E.

1976-08-01

Certain systems use hermetically-sealed multipin connectors welded into a stainless steel support ring. Failure of these hermetic seals during welding continues to be a problem, and similar problems are anticipated on advanced systems. Since the assembly is expensive, and the detection, prevention, and repair of hermetic seal failures is costly, development of an improved welding process is important. Extended service life also requires a lower system leak rate, thus causing an increased need for maintaining the hermetic seal without supplemental epoxy sealing and repair. Experience shows that up to 70 percent of the 10-pin SA1810-2 connectors have gross leaks (greatermore » than 0.003 mm/sup 3//s Standard Temperature and Pressure (STP)) after being welded using established welding processes and without using the epoxy pre-seal process. Acceptable leak rates of less than 0.00001 mm/sup 3//s STP were achieved from 20 SA1810-2 10-pin connectors using heat sinks and an intermittent gas-tungsten-arc (GTA) weld technique. The process developed consists of using a massive copper heat sink with silicon thermal joint compound to maintain control of temperature in the hermetic seal area and using a 12-segment GTA weld with compressed argon gas cooling between weld segments. The process and techniques developed are considered acceptable for welding the SA1810 family of connectors.« less

Vortex Shedding in the Wake Induced by a Real Elephant Seal Whisker

NASA Astrophysics Data System (ADS)

Turk, Jodi; Omilion, Alexis; Zhang, Wei; Kim, Jeong-Jae; Kim, Jeong-Ju; Choi, Woo-Rak; Lee, Sang-Joon

2017-11-01

Biomimicry has been adopted to create innovative solutions in a vast range of applications. One such application is the design of seal-whisker-inspired flow sensors for autonomous underwater vehicles (AUVs). In dark, cramped, and unstable terrain AUVs are not able to maneuver using visual and sonar-based navigation. Hence, it is critical to use underwater flow sensors to accurately detect minute disturbances in the surroundings. Certain seal whiskers exhibit a unique undulating three-dimensional morphology that can reduce vortex induced vibrations (VIVs) if the major axis of the whisker cross-section is aligned to the inflow. This allows the seal to precisely track prey fish upstream using solely their whiskers. The current study aims to understand the effect of a real seal whisker's morphology on the vortex shedding behavior. Despite extensive studies of wake induced by scaled whisker-like models, the vortex shedding in the wake of a real seal whisker is not well understood. A series of experiments are conducted with a high-speed Particle Imaging Velocimetry (PIV) system in a water channel to examine the vortex shedding downstream from a smooth whisker and an undulating whisker at a Reynolds number of a few hundred. Results of the vortex shedding induced by real seal whiskers can provide insights on developing high-sensitivity underwater flow sensors for AUVs and other whisker-inspired structures.

Deepwater Program: Exploration and Research of Northern Gulf of Mexico Deepwater Natural and Artificial Hard Bottom Habitats with Emphasis on Coral Communities: Reefs, Rigs and Wrecks

DTIC Science & Technology

2012-01-01

Coral Communities: Reefs, Rigs and Wrecks Project Director - James Brooks TDI-Brooks International, 1902 Pinon, College Station, TX 77845 Phone...Reefs, Rigs and Wrecks 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK...geological/geophysical and oceanographic studies, Mr. Dan Warren and Mr. Rob Church from C&C Technology coordinate the wrecks studies along with, Dr

Magnetic Suspension for Dynamic Spin Rig

NASA Technical Reports Server (NTRS)

Johnson, Dexter

1998-01-01

NASA Lewis Research Center's Dynamic Spin Rig, located in Building 5, Test Cell CW-18, is used to test turbomachinery blades and components by rotating them in a vacuum chamber. A team from Lewis' Machine Dynamics Branch successfully integrated a magnetic bearing and control system into the Dynamic Spin Rig. The magnetic bearing worked very well both to support and shake the shaft. It was demonstrated that the magnetic bearing can transmit more vibrational energy into the shaft and excite some blade modes to larger amplitudes than the existing electromagnetic shakers can.

Stress-life relation of the rolling-contact fatigue spin rig

NASA Technical Reports Server (NTRS)

Butler, Robert H; Carter, Thomas L

1957-01-01

The rolling-contact fatigue spin rig was used to test groups of SAE 52100 9.16-inch-diameter balls lubricated with a mineral oil at 600,000-, 675,000-, and 750,000-psi maximum Hertz stress. Cylinders of AISI M-1 vacuum and commercial melts and MV-1 (AISI M-50) were used as race specimens. Stress-life exponents produced agree closely with values accepted in industry. The type of failure obtained in the spin rig was similar to the subsurface fatigue spells found in bearings.

The Mechanical Performance of Subscale Candidate Elastomer Docking Seals

NASA Technical Reports Server (NTRS)

Bastrzyk, Marta B.; Daniels, Christopher C.

2010-01-01

The National Aeronautics and Space Administration is developing a Low Impact Docking System (LIDS) for future exploration missions. The mechanism is a new state-of-the-art device for in-space assembly of structures and rendezvous of vehicles. At the interface between two pressurized modules, each with a version of the LIDS attached, a composite elastomer-metal seal assembly prevents the breathable air from escaping into the vacuum of space. Attached to the active LIDS, this seal mates against the passive LIDS during docking operation. The main interface seal assembly must exhibit low leak and outgas values, must be able to withstand various harsh space environments, must remain operational over a range of temperatures from -50 C to 75 C, and perform after numerous docking cycles. This paper presents results from a comprehensive study of the mechanical performance of four candidate subscale seal assembly designs at -50, 23, 50, and 75 C test temperatures. In particular, the force required to fully compress the seal during docking, and that which is required for separation during the undocking operation were measured. The height of subscale main interface seal bulbs, as well as the test temperature, were shown to have a significant effect on the forces the main interface seal of the LIDS may experience during docking and undocking operations. The average force values required to fully compress each of the seal assemblies were shown to increase with test temperature by approximately 50% from -50 to 75 C. Also, the required compression forces were shown to increase as the height of the seal bulb was increased. The seal design with the tallest elastomer seal bulb, which was 31% taller than that with the shortest bulb, required force values approximately 45% higher than those for the shortest bulb, independent of the test temperature. The force required to separate the seal was shown to increase with decreasing temperature after 15 hours of simulated docking. No adhesion force was observed at 75 C, while magnitudes of up to 235 lbf were recorded at the refrigerated temperature. In addition, the adhesion force was observed to increase with bulb height. When compared with the LIDS program requirements, the measured compression force values were found to be below the maximum allowable load allotted to the main interface seal. However, the measured adhesion force values at the refrigerated test temperature were found to exceed the program limits.

Evolution of the DEAD box helicase family in chicken: chickens have no DHX9 ortholog.

PubMed

Sato, Haruko; Oshiumi, Hiroyuki; Takaki, Hiromi; Hikono, Hirokazu; Seya, Tsukasa

2015-10-01

Viral RNA represents a pattern molecule that can be recognized by RNA sensors in innate immunity. Humans and mice possess cytoplasmic DNA/RNA sensors for detecting viral replication. There are a number of DEAD (Asp-Glu-Ala-Asp; DExD/H) box-type helicases in mammals, among which retinoic acid-inducible gene 1 (RIG-I) and melanoma differentiation-associated protein 5 (MDA50) are indispensable for RNA sensing; however, they are functionally supported by a number of sensors that directly bind viral RNA or replicative RNA intermediates to convey signals to RIG-I and MDA5. Some DEAD box helicase members recognize DNA irrespective of the origin. These sensors transmit IFN-inducing signals through adaptors, including mitochondrial antiviral signaling. Viral double-stranded RNAs are reportedly sensed by the helicases DDX1, DDX21, DHX36, DHX9, DDX3, DDX41, LGP2 and DDX60, in addition to RIG-I and MDA5, and induce type I IFNs, thereby blocking viral replication. Humans and mice have all nucleic acid sensors listed here. In the RNA sensing system in chicken, it was found in the present study that most DEAD box helicases are conserved; however, DHX9 is genetically deficient in addition to reported RIG-I. Based on the current genome databases, similar DHX9 deficiency was observed in ducks and several other bird species. Because chicken, but not duck, was found to be deficient in RIG-I, the RNA-sensing system of chicken lacks RIG-I and DHX9 and is thus more fragile than that of duck or mammal. DHX9 may generally compensate for the function of RIG-I and deficiency of DHX9 possibly participates in exacerbations of viral infection such as influenza in chickens. © 2015 The Societies and Wiley Publishing Asia Pty Ltd.

Non-technical skills: enhancing safety in operating theatres (and drilling rigs).

PubMed

Flin, Rhona

2014-03-01

On April 20th 2010, a large Transocean drilling rig called the Deepwater Horizon was operating in the Gulf of Mexico to drill the Macondo well, for the oil company BP. The job was six weeks behind schedule and $58 million over budget and had not been without difficulty: it was a high pressure well, 2.5 miles below the seabed. At 5.45 am, the Halliburton cementing engineer sent an email to say: 'We have completed the job and it went well'. At 9.43 pm, 16 hours later, there was a release of hydrocarbons into the well bore and the drilling rig experienced a catastrophic blowout as the high pressure oil and gas escaped onto the rig and into the ocean. The resulting explosions and fire killed 11 of the crew of 126, injured many more and created an enormous oil spill across the Gulf.

Study on Silicon Microstructure Processing Technology Based on Porous Silicon

NASA Astrophysics Data System (ADS)

Shang, Yingqi; Zhang, Linchao; Qi, Hong; Wu, Yalin; Zhang, Yan; Chen, Jing

2018-03-01

Aiming at the heterogeneity of micro - sealed cavity in silicon microstructure processing technology, the technique of preparing micro - sealed cavity of porous silicon is proposed. The effects of different solutions, different substrate doping concentrations, different current densities, and different etching times on the rate, porosity, thickness and morphology of the prepared porous silicon were studied. The porous silicon was prepared by different process parameters and the prepared porous silicon was tested and analyzed. For the test results, optimize the process parameters and experiments. The experimental results show that the porous silicon can be controlled by optimizing the parameters of the etching solution and the doping concentration of the substrate, and the preparation of porous silicon with different porosity can be realized by different doping concentration, so as to realize the preparation of silicon micro-sealed cavity, to solve the sensor sensitive micro-sealed cavity structure heterogeneous problem, greatly increasing the application of the sensor.

Demonstrating Phase Changes.

ERIC Educational Resources Information Center

Rohr, Walter

1995-01-01

Presents two experiments that demonstrate phase changes. The first experiment explores phase changes of carbon dioxide using powdered dry ice sealed in a piece of clear plastic tubing. The second experiment demonstrates an equilibrium process in which a crystal grows in equilibrium with its saturated solution. (PVD)

Modulations of Driven Nonlinear Surface Waves on Water and Liquid Helium-4.

DTIC Science & Technology

1985-06-01

loop to control drive amplitude. 63 CHAPTER III. DATA AND INTERPRETATION. Section 5. Cockscombs. 25. Cockscomb in water at f/30. 67 26. Double...annular trough, sealed and then partially filled with normal or superfluid helium, is oscillated vertically and supported 6 . . ... . . . . . 41 700...44 0 .0 0 00 loudspeaker surface wave sealed trough transducer shel oo •Figure 3. Schmatic of the helium experiment. 8 r

Further Investigations of High Temperature Knitted Spring Tubes for Advanced Control Surface Seal Applications

NASA Technical Reports Server (NTRS)

Taylor, Shawn C.; DeMange, Jeffrey J.; Dunlap, Patrick H., Jr.; Steinetz, Bruce M.

2006-01-01

Knitted metallic spring tubes are the structural backbones that provide resiliency in control surface seals for use on current and future reusable space launch vehicles. Control surface seals fill the space between movable control surfaces such as body flaps, rudders and elevons, and the static body structures to which they are attached. These seals must remain in continuous contact with opposing surfaces to prevent the ingestion of damaging hot gases encountered during atmospheric re-entry. The Inconel X-750 (Special Metals Corporation) spring tube utilized in the baseline control surface seal shows significant resiliency loss when compressed at temperatures as low as 1200 F. High temperature compression testing and microstructural analysis show that creep is the dominant deformation mechanism leading to permanent set and resiliency loss in tested spring tube samples. Additional evaluation using a structured design of experiments approach shows that spring tube performance, primarily high temperature resiliency, can be enhanced through material substitution of Rene 41 (Allvac) alloy (for the baseline Inconel X-750 material) when coupled with specialized thermal processing.

An experimental investigation of rubbing interaction in labyrinth seals at cryogenic temperature

NASA Technical Reports Server (NTRS)

Dolan, F. X.; Kennedy, F. E.; Schulson, E. M.

1985-01-01

An experimental program was carried out to address issues related to the observed cracking of the titanium knife edges on the labyrinth seals of the high pressure fuel pump (HPFP) in the Space Shuttle main engine (SSME). Thermal shock experiments were carried out using a jet specimen with geometry similar to the knife edge geometry. These tests demonstrate that cracking of the titanium alloy is possible in a situation involving repeated thermal cycles over a wide temperature range, as might be realized during a rub in the liquid hydrogen fuel pump. High speed rub interaction tests were conducted using a representative knife edge and seal geometry over a broad range of interaction rates. Alternative materials were also experimentally evaluated. These tests provide information which can be used to design improved labyrinth seals for the HPFP of the SSME. In particular, plasma-sprayed aluminum-graphite was found to be significantly better than aluminum alloy seals used at present from the standpoint of rub performance. Ion nitriding of the titanium alloy knife edges was also found to improve rub performance compared with the untreated baseline knife edge material.

Influence of inner circular sealing area impression method on the retention of complete dentures.

PubMed

Wang, Cun-Wei; Shao, Qi; Sun, Hui-Qiang; Mao, Meng-Yun; Zhang, Xin-Wei; Gong, Qi; Xiao, Guo-Ning

2015-01-01

The aims of the present study were to describe an impression method of "inner circular sealing area" and to evaluate the effect of the method on retention, aesthetics and comfort of complete dentures, which lack labial base for patients with maxillary protrusions. Three patients were subjected to the experiment, and two sets of complete maxillary dentures were made for each patient; the first set was made without labial base via an inner circular sealing area method (experimental group) and the second had an intact base that was made with conventional methods (control group). Retention force tests were implemented with a tensile strength assessment device to assess the retention and a visual analogue scale (VAS) was used to evaluate the comfort between the two groups. Results showed larger retention force, better aesthetics and more comfort in the experimental group. The improved two-step impression method formed an inner circular sealing area that prevented damage to the peripheral border seal effect of the denture caused by incomplete bases and obtained better denture retention.

The ubiquitin-specific protease USP15 promotes RIG-I-mediated antiviral signaling by deubiquitylating TRIM25.

PubMed

Pauli, Eva-Katharina; Chan, Ying Kai; Davis, Meredith E; Gableske, Sebastian; Wang, May K; Feister, Katharina F; Gack, Michaela U

2014-01-07

Ubiquitylation is an important mechanism for regulating innate immune responses to viral infections. Attachment of lysine 63 (Lys(63))-linked ubiquitin chains to the RNA sensor retinoic acid-inducible gene-I (RIG-I) by the ubiquitin E3 ligase tripartite motif protein 25 (TRIM25) leads to the activation of RIG-I and stimulates production of the antiviral cytokines interferon-α (IFN-α) and IFN-β. Conversely, Lys(48)-linked ubiquitylation of TRIM25 by the linear ubiquitin assembly complex (LUBAC) stimulates the proteasomal degradation of TRIM25, thereby inhibiting the RIG-I signaling pathway. Here, we report that ubiquitin-specific protease 15 (USP15) deubiquitylates TRIM25, preventing the LUBAC-dependent degradation of TRIM25. Through protein purification and mass spectrometry analysis, we identified USP15 as an interaction partner of TRIM25 in human cells. Knockdown of endogenous USP15 by specific small interfering RNA markedly enhanced the ubiquitylation of TRIM25. In contrast, expression of wild-type USP15, but not its catalytically inactive mutant, reduced the Lys(48)-linked ubiquitylation of TRIM25, leading to its stabilization. Furthermore, ectopic expression of USP15 enhanced the TRIM25- and RIG-I-dependent production of type I IFN and suppressed RNA virus replication. In contrast, depletion of USP15 resulted in decreased IFN production and markedly enhanced viral replication. Together, these data identify USP15 as a critical regulator of the TRIM25- and RIG-I-mediated antiviral immune response, thereby highlighting the intricate regulation of innate immune signaling.

In Vivo Ligands of MDA5 and RIG-I in Measles Virus-Infected Cells

PubMed Central

Hembach, Katharina; Baum, Alina; García-Sastre, Adolfo; Söding, Johannes; Conzelmann, Karl-Klaus

2014-01-01

RIG-I-like receptors (RLRs: RIG-I, MDA5 and LGP2) play a major role in the innate immune response against viral infections and detect patterns on viral RNA molecules that are typically absent from host RNA. Upon RNA binding, RLRs trigger a complex downstream signaling cascade resulting in the expression of type I interferons and proinflammatory cytokines. In the past decade extensive efforts were made to elucidate the nature of putative RLR ligands. In vitro and transfection studies identified 5′-triphosphate containing blunt-ended double-strand RNAs as potent RIG-I inducers and these findings were confirmed by next-generation sequencing of RIG-I associated RNAs from virus-infected cells. The nature of RNA ligands of MDA5 is less clear. Several studies suggest that double-stranded RNAs are the preferred agonists for the protein. However, the exact nature of physiological MDA5 ligands from virus-infected cells needs to be elucidated. In this work, we combine a crosslinking technique with next-generation sequencing in order to shed light on MDA5-associated RNAs from human cells infected with measles virus. Our findings suggest that RIG-I and MDA5 associate with AU-rich RNA species originating from the mRNA of the measles virus L gene. Corresponding sequences are poorer activators of ATP-hydrolysis by MDA5 in vitro, suggesting that they result in more stable MDA5 filaments. These data provide a possible model of how AU-rich sequences could activate type I interferon signaling. PMID:24743923

The Ubiquitin-Specific Protease USP15 Promotes RIG-I–Mediated Antiviral Signaling by Deubiquitylating TRIM25

PubMed Central

Pauli, Eva-Katharina; Chan, Ying Kai; Davis, Meredith E.; Gableske, Sebastian; Wang, May K.; Feister, Katharina F.; Gack, Michaela U.

2014-01-01

Ubiquitylation is an important mechanism for regulating innate immune responses to viral infections. Attachment of lysine 63 (Lys63)–linked ubiquitin chains to the RNA sensor retinoic acid–inducible gene-I (RIG-I) by the ubiquitin E3 ligase tripartite motif protein 25 (TRIM25) leads to the activation of RIG-I and stimulates production of the antiviral cytokines interferon-α (IFN-α) and IFN-β. Conversely, Lys48-linked ubiquitylation of TRIM25 by the linear ubiquitin assembly complex (LUBAC) stimulates the proteasomal degradation of TRIM25, thereby inhibiting the RIG-I signaling pathway. Here, we report that ubiquitin-specific protease 15 (USP15) deubiquitylates TRIM25, preventing the LUBAC-dependent degradation of TRIM25. Through protein purification and mass spectrometry analysis, we identified USP15 as an interaction partner of TRIM25 in human cells. Knockdown of endogenous USP15 by specific small interfering RNA markedly enhanced the ubiquitylation of TRIM25. In contrast, expression of wild-type USP15, but not its catalytically inactive mutant, reduced the Lys48-linked ubiquitylation of TRIM25, leading to its stabilization. Furthermore, ectopic expression of USP15 enhanced the TRIM25- and RIG-I–dependent production of type I IFN and suppressed RNA virus replication. In contrast, depletion of USP15 resulted in decreased IFN production and markedly enhanced viral replication. Together, these data identify USP15 as a critical regulator of the TRIM25- and RIG-I–mediated antiviral immune response, thereby highlighting the intricate regulation of innate immune signaling. PMID:24399297

Molecular Mechanism of Signal Perception and Integration by the Innate Immune Sensor Retinoic Acid-inducible Gene-I (RIG-I)*

PubMed Central

Binder, Marco; Eberle, Florian; Seitz, Stefan; Mücke, Norbert; Hüber, Christian M.; Kiani, Narsis; Kaderali, Lars; Lohmann, Volker; Dalpke, Alexander; Bartenschlager, Ralf

2011-01-01

RIG-I is a major innate immune sensor for viral infection, triggering an interferon (IFN)-mediated antiviral response upon cytosolic detection of viral RNA. Double-strandedness and 5′-terminal triphosphates were identified as motifs required to elicit optimal immunological signaling. However, very little is known about the response dynamics of the RIG-I pathway, which is crucial for the ability of the cell to react to diverse classes of viral RNA while maintaining self-tolerance. In the present study, we addressed the molecular mechanism of RIG-I signal detection and its translation into pathway activation. By employing highly quantitative methods, we could establish the length of the double-stranded RNA (dsRNA) to be the most critical determinant of response strength. Size exclusion chromatography and direct visualization in scanning force microscopy suggested that this was due to cooperative oligomerization of RIG-I along dsRNA. The initiation efficiency of this oligomerization process critically depended on the presence of high affinity motifs, like a 5′-triphosphate. It is noteworthy that for dsRNA longer than 200 bp, internal initiation could effectively compensate for a lack of terminal triphosphates. In summary, our data demonstrate a very flexible response behavior of the RIG-I pathway, in which sensing and integration of at least two distinct signals, initiation efficiency and double strand length, allow the host cell to mount an antiviral response that is tightly adjusted to the type of the detected signal, such as viral genomes, replication intermediates, or small by-products. PMID:21659521

Study of the effect of bacteria on the disappearance and transformation of CO in the sealed fire zone of coal mine

NASA Astrophysics Data System (ADS)

Zhai, Xiaowei; Wu, Shibo; Deng, Jun; Yang, Yifan; Jiang, Hua; Wang, Kai

2017-01-01

When the underground coal mine gob area has been sealed due to the coal spontaneous combustion, under the low oxygen and potentially high temperature environment, the CO concentration could drop sharply and disappear quickly. But it could rise rapidly after re-opening. These indicate that the disappearance is the only index for coal burnt out. In order to find a way how let CO disappear, experiments have been conducted using the newly developed experiment setup for three samples, raw, watered and bacteria-free coal sample. The CO and CO2 concentration have been monitored and analyzed. The results show the bacteria in the coal do consume CO and increase the chance of CO transfer to CO2. These results reveal how let CO disappear in a sealed zone from a new aspect. And the accuracy was improved when used gas index to determine combustion status for coal spontaneous combustion.

Whirl measurements on leakage flows in turbomachine models

NASA Technical Reports Server (NTRS)

Addlesee, A. J.; Altiparmak, D.; Pan, S.

1994-01-01

The beneficial effects claimed for whirl control devices demonstrate that the dynamic behavior of rotors is influenced by the fluid whirl in shaft and balance drum seals. The present paper reports results from two series of experiments, the first on the factors affecting the whirl at the seal inlet, and the second on the variation of whirl velocity along the seal. In both cases the LDA measurement technique required the clearance between the fixed and rotating parts of the models to be substantially greater than occurs in real machines, but the results are indicative nevertheless. Experimental and theoretical results are given for the radial distribution of whirl velocity in the gap between impeller shroud and pump casing. Results of tests with modified stator surfaces are also shown. This work leads naturally into the second series of experiments where some preliminary measurements of velocity distribution in the clearance between a fixed stator and a rotating shaft are reported for a range of inlet whirl conditions.

Downregulation of MicroRNA miR-526a by Enterovirus Inhibits RIG-I-Dependent Innate Immune Response

PubMed Central

Xu, Changzhi; He, Xiang; Zheng, Zirui; Zhang, Zhe; Wei, Congwen; Guan, Kai; Hou, Lihua; Zhang, Buchang; Zhu, Lin; Cao, Yuan; Zhang, Yanhong; Cao, Ye; Ma, Shengli; Wang, Penghao; Zhang, Pingping; Xu, Quanbin; Ling, Youguo

2014-01-01

ABSTRACT Retinoic acid-inducible gene I (RIG-I) is an intracellular RNA virus sensor that induces type I interferon-mediated host-protective innate immunity against viral infection. Although cylindromatosis (CYLD) has been shown to negatively regulate innate antiviral response by removing K-63-linked polyubiquitin from RIG-I, the regulation of its expression and the underlying regulatory mechanisms are still incompletely understood. Here we show that RIG-I activity is regulated by inhibition of CYLD expression mediated by the microRNA miR-526a. We found that viral infection specifically upregulates miR-526a expression in macrophages via interferon regulatory factor (IRF)-dependent mechanisms. In turn, miR-526a positively regulates virus-triggered type I interferon (IFN-I) production, thus suppressing viral replication, the underlying mechanism of which is the enhancement of RIG-I K63-linked ubiquitination by miR-526a via suppression of the expression of CYLD. Remarkably, virus-induced miR-526a upregulation and CYLD downregulation are blocked by enterovirus 71 (EV71) 3C protein, while ectopic miR-526a expression inhibits the replication of EV71 virus. The collective results of this study suggest a novel mechanism of the regulation of RIG-I activity during RNA virus infection by miR-526a and suggest a novel mechanism for the evasion of the innate immune response controlled by EV71. IMPORTANCE RNA virus infection upregulates the expression of miR-526a in macrophages through IRF-dependent pathways. In turn, miR-526a positively regulates virus-triggered type I IFN production and inhibits viral replication, the underlying mechanism of which is the enhancement of RIG-I K-63 ubiquitination by miR-526a via suppression of the expression of CYLD. Remarkably, virus-induced miR-526a upregulation and CYLD downregulation are blocked by enterovirus 71 (EV71) 3C protein; cells with overexpressed miR-526a were highly resistant to EV71 infection. The collective results of this study suggest a novel mechanism of the regulation of RIG-I activity during RNA virus infection by miR-526a and propose a novel mechanism for the evasion of the innate immune response controlled by EV71. PMID:25056901

DOE Office of Scientific and Technical Information (OSTI.GOV)

Leigh, Christi D.; Hansen, Francis D.

This report summarizes the state of salt repository science, reviews many of the technical issues pertaining to disposal of heat-generating nuclear waste in salt, and proposes several avenues for future science-based activities to further the technical basis for disposal in salt. There are extensive salt formations in the forty-eight contiguous states, and many of them may be worthy of consideration for nuclear waste disposal. The United States has extensive experience in salt repository sciences, including an operating facility for disposal of transuranic wastes. The scientific background for salt disposal including laboratory and field tests at ambient and elevated temperature, principlesmore » of salt behavior, potential for fracture damage and its mitigation, seal systems, chemical conditions, advanced modeling capabilities and near-future developments, performance assessment processes, and international collaboration are all discussed. The discussion of salt disposal issues is brought current, including a summary of recent international workshops dedicated to high-level waste disposal in salt. Lessons learned from Sandia National Laboratories' experience on the Waste Isolation Pilot Plant and the Yucca Mountain Project as well as related salt experience with the Strategic Petroleum Reserve are applied in this assessment. Disposal of heat-generating nuclear waste in a suitable salt formation is attractive because the material is essentially impermeable, self-sealing, and thermally conductive. Conditions are chemically beneficial, and a significant experience base exists in understanding this environment. Within the period of institutional control, overburden pressure will seal fractures and provide a repository setting that limits radionuclide movement. A salt repository could potentially achieve total containment, with no releases to the environment in undisturbed scenarios for as long as the region is geologically stable. Much of the experience gained from United States repository development, such as seal system design, coupled process simulation, and application of performance assessment methodology, helps define a clear strategy for a heat-generating nuclear waste repository in salt.« less

Soil seal development under simulated rainfall: Structural, physical and hydrological dynamics

NASA Astrophysics Data System (ADS)

Armenise, Elena; Simmons, Robert W.; Ahn, Sujung; Garbout, Amin; Doerr, Stefan H.; Mooney, Sacha J.; Sturrock, Craig J.; Ritz, Karl

2018-01-01

This study delivers new insights into rainfall-induced seal formation through a novel approach in the use of X-ray Computed Tomography (CT). Up to now seal and crust thickness have been directly quantified mainly through visual examination of sealed/crusted surfaces, and there has been no quantitative method to estimate this important property. X-ray CT images were quantitatively analysed to derive formal measures of seal and crust thickness. A factorial experiment was established in the laboratory using open-topped microcosms packed with soil. The factors investigated were soil type (three soils: silty clay loam - ZCL, sandy silt loam - SZL, sandy loam - SL) and rainfall duration (2-14 min). Surface seal formation was induced by applying artificial rainfall events, characterised by variable duration, but constant kinetic energy, intensity, and raindrop size distribution. Soil porosities derived from CT scans were used to quantify the thickness of the rainfall-induced surface seals and reveal temporal seal micro-morphological variations with increasing rainfall duration. In addition, the water repellency and infiltration dynamics of the developing seals were investigated by measuring water drop penetration time (WDPT) and unsaturated hydraulic conductivity (Kun). The range of seal thicknesses detected varied from 0.6 to 5.4 mm. Soil textural characteristics and OM content played a central role in the development of rainfall-induced seals, with coarser soil particles and lower OM content resulting in thicker seals. Two different trends in soil porosity vs. depth were identified: i) for SL soil porosity was lowest at the immediate soil surface, it then increased constantly with depth till the median porosity of undisturbed soil was equalled; ii) for ZCL and SL the highest reduction in porosity, as compared to the median porosity of undisturbed soil, was observed in a well-defined zone of maximum porosity reduction c. 0.24-0.48 mm below the soil surface. This contrasting behaviour was related to different dynamics and processes of seal formation which depended on the soil properties. The impact of rainfall-induced surface sealing on the hydrological behaviour of soil (as represented by WDTP and Kun) was rapid and substantial: an average 60% reduction in Kun occurred for all soils between 2 and 9 min rainfall, and water repellent surfaces were identified for SZL and ZCL. This highlights that the condition of the immediate surface of agricultural soils involving rainfall-induced structural seals has a strong impact in the overall ability of soil to function as water reservoir.

Russian EVA 34

NASA Image and Video Library

2013-08-16

ISS036-E-033400 (16 Aug. 2013) --- Russian cosmonaut Alexander Misurkin (lower left), Expedition 36 flight engineer, attired in a Russian Orlan spacesuit, participates in a session of extravehicular activity (EVA) to continue outfitting the International Space Station. During the seven-hour, 29-minute spacewalk ? the longest ever conducted by a pair of Russian cosmonauts ? Misurkin and Fyodor Yurchikhin (out of frame) rigged cables for the future arrival of a Russian laboratory module and installed an experiment panel.

Russian EVA 34

NASA Image and Video Library

2013-08-16

ISS036-E-033402 (16 Aug. 2013) --- Russian cosmonaut Alexander Misurkin (lower left), Expedition 36 flight engineer, attired in a Russian Orlan spacesuit, participates in a session of extravehicular activity (EVA) to continue outfitting the International Space Station. During the seven-hour, 29-minute spacewalk ? the longest ever conducted by a pair of Russian cosmonauts ? Misurkin and Fyodor Yurchikhin (out of frame) rigged cables for the future arrival of a Russian laboratory module and installed an experiment panel.

A high-speed photography study of cavitation in a dynamically loaded journal bearing

NASA Technical Reports Server (NTRS)

Sun, D. C.; Brewe, D. E.

1990-01-01

The earlier study made by Jacobson and Hamrock on the cavitation of liquid lubricant films in a dynamically loaded journal bearing was repeated with a quartz sleeve, which was more rigid than the Polymethylmethacrylate (PMMA) sleeve used previously. Various improvements of the test rig were made concomitantly so that the experimental errors could be better controlled and assessed. The updated speed photography experiment and its results are described.

A high speed photography study of cavitation in a dynamically loaded journal bearing

NASA Technical Reports Server (NTRS)

Sun, D. C.; Brewe, D. E.

1991-01-01

The earlier study made by Jacobson and Hamrock on the cavitation of liquid lubricant films in a dynamically loaded journal bearing was repeated with a quartz sleeve, which was more rigid than the Polymethylmethacrylate (PMMA) sleeve used previously. Various improvements of the test rig were made concomitantly so that the experimental errors could be better controlled and assessed. The updated speed photography experiment and its results are described.

Paresev 1 in Landing

NASA Image and Video Library

1962-02-05

Pilot and Paresev 1 preparing for a landing on the Rogers dry lakebed in 1962 at Edwards Air Force Base, California. The flight program began with ground tow tests. Several tows were made before liftoff was attempted to check the control rigging and to familiarize the pilot with the vehicle’s ground stability. As the pilot’s confidence and experience increased, tow speeds were also increased until liftoff was attained. Liftoff was at about 40 knots indicated airspeed (kias).

Underwater auditory localization by a swimming harbor seal (Phoca vitulina).

PubMed

Bodson, Anais; Miersch, Lars; Mauck, Bjoern; Dehnhardt, Guido

2006-09-01

The underwater sound localization acuity of a swimming harbor seal (Phoca vitulina) was measured in the horizontal plane at 13 different positions. The stimulus was either a double sound (two 6-kHz pure tones lasting 0.5 s separated by an interval of 0.2 s) or a single continuous sound of 1.2 s. Testing was conducted in a 10-m-diam underwater half circle arena with hidden loudspeakers installed at the exterior perimeter. The animal was trained to swim along the diameter of the half circle and to change its course towards the sound source as soon as the signal was given. The seal indicated the sound source by touching its assumed position at the board of the half circle. The deviation of the seals choice from the actual sound source was measured by means of video analysis. In trials with the double sound the seal localized the sound sources with a mean deviation of 2.8 degrees and in trials with the single sound with a mean deviation of 4.5 degrees. In a second experiment minimum audible angles of the stationary animal were found to be 9.8 degrees in front and 9.7 degrees in the back of the seal's head.

Flow Induced Spring Coefficients of Labyrinth Seals for Application in Rotor Dynamics

NASA Technical Reports Server (NTRS)

Benckert, H.; Wachter, J.

1980-01-01

Flow induced aerodynamic spring coefficients of labyrinth seals are discussed and the restoring force in the deflection plane of the rotor and the lateral force acting perpendicularly to it are also considered. The effects of operational conditions on the spring characteristics of these components are examined, such as differential pressure, speed, inlet flow conditions, and the geometry of the labyrinth seals. Estimation formulas for the lateral forces due to shaft rotation and inlet swirl, which are developed through experiments, are presented. The utilization of the investigations is explained and results of stability calculations, especially for high pressure centrifugal compressors, are added. Suggestions are made concerning the avoidance of exciting forces in labyrinths.

Enabling two-phase microfluidic thermal transport systems using a novel thermal-flux degassing and fluid charging approach

NASA Astrophysics Data System (ADS)

Singh Dhillon, Navdeep; Pisano, Albert P.

2014-03-01

A novel two-port thermal-flux method has been proposed and demonstrated for degassing and charging two-phase microfluidic thermal transport systems with a degassed working fluid. In microscale heat pipes and loop heat pipes (mLHPs), small device volumes and large capillary forces associated with smaller feature sizes render conventional vacuum pump-based degassing methods quite impractical. Instead, we employ a thermally generated pressure differential to purge non-condensable gases from these devices before charging them with a degassed working fluid in a two-step process. Based on the results of preliminary experiments studying the effectiveness and reliability of three different high temperature-compatible device packaging approaches, an optimized compression packaging technique was developed to degas and charge a mLHP device using the thermal-flux method. An induction heating-based noninvasive hermetic sealing approach for permanently sealing the degassed and charged mLHP devices has also been proposed. To demonstrate the efficacy of this approach, induction heating experiments were performed to noninvasively seal 1 mm square silicon fill-hole samples with donut-shaped solder preforms. The results show that the minimum hole sealing induction heating time is heat flux limited and can be estimated using a lumped capacitance thermal model. However, further continued heating of the solder uncovers the hole due to surface tension-induced contact line dynamics of the molten solder. It was found that an optimum mass of the solder preform is required to ensure a wide enough induction-heating time window for successful sealing of a fill-hole.

Design of the Madison Dynamo Experiment

NASA Astrophysics Data System (ADS)

Kendrick, R. D.; Forest, C. B.; O'Connell, R.; Nornberg, M. D.; Spence, E. J.

2004-11-01

A spherical dynamo experiment has been constructed at the University of Wisconsin's liquid-sodium facility. The goals of the experiment are to observe and understand magnetic instabilities driven by flow shear in MHD systems, investigate MHD turbulence for magnetic Reynolds numbers of 100, and understand the role of fluid turbulence in current generation. Magnetic field generation is possible for only specific flow geometries. The experiment consists of a 1 m diameter, spherical stainless steel vessel filled with liquid sodium at 110 Celsius. The temperature of the vessel is maintained through an actively-heated-and-cooled oil heat-exchange system. Two 100 Hp motors with impellers drive flows in the liquid sodium with flow velocities near 15 m/s. Each shaft is sealed with an oil-buffered dual mechanical cartridge seal. The experiment is automated for remote operation and data logging. The melting and transfer of one metric ton of sodium to a storage vessel is discussed. Operating parameters and performance of the experiment are presented.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This article describes a unique rig designed by SEDCO FOREX operating in the Paris basin of France. Built to drill clusters of wells from a single pad, Rig 47 significantly reduces the time needed to move from well to well on a pad and from location to location.

3. EASTERN VIEW OF HOISTING RIG FOR OXYGEN LANCES ON ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. EASTERN VIEW OF HOISTING RIG FOR OXYGEN LANCES ON THE FLUX STORAGE FLOOR OF THE FURNACE AISLE IN THE BOP SHOP. - U.S. Steel Duquesne Works, Basic Oxygen Steelmaking Plant, Along Monongahela River, Duquesne, Allegheny County, PA

Test rig and particulate deposit and cleaning evaluation processes using the same

DOEpatents

Schroder, Mark Stewart; Woodmansee, Donald Ernest; Beadie, Douglas Frank

2002-01-01

A rig and test program for determining the amount, if any, of contamination that will collect in the passages of a fluid flow system, such as a power plant fluid delivery system to equipment assemblies or sub-assemblies, and for establishing methods and processes for removing contamination therefrom. In the presently proposed embodiment, the rig and test programs are adapted in particular to utilize a high-pressure, high-volume water flush to remove contamination from substantially the entire fluid delivery system, both the quantity of contamination and as disposed or deposited within the system.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Akhrymuk, Ivan; Frolov, Ilya; Frolova, Elena I., E-mail: evfrolova@UAB.edu

Alphaviruses are a family of positive-strand RNA viruses that circulate on all continents between mosquito vectors and vertebrate hosts. Despite a significant public health threat, their biology is not sufficiently investigated, and the mechanisms of alphavirus replication and virus–host interaction are insufficiently understood. In this study, we have applied a variety of experimental systems to further understand the mechanism by which infected cells detect replicating alphaviruses. Our new data strongly suggest that activation of the antiviral response by alphavirus-infected cells is determined by the integrity of viral genes encoding proteins with nuclear functions, and by the presence of two cellularmore » pattern recognition receptors (PRRs), RIG-I and MDA5. No type I IFN response is induced in their absence. The presence of either of these PRRs is sufficient for detecting virus replication. However, type I IFN activation in response to pathogenic alphaviruses depends on the basal levels of RIG-I or MDA5. - Highlights: • Both RIG-I and MDA5 detect alphavirus replication. • Alphavirus-induced transcriptional shutoff affects type I IFN induction. • Sensing of alphavirus replication by RIG-I and MDA5 depends on their concentrations. • High basal level of RIG-I and MDA5 allows IFN induction by pathogenic alphaviruses. • This dependence determines the discrepancy between the in vivo and in vitro data.« less

Major mishaps among mobile offshore drilling units, 1955-1981: time trends and fatalities.

PubMed

Clemmer, D I; Diem, J E

1985-03-01

Major mishaps among mobile offshore drilling units worldwide from 1955-1981 were identified from industry and government sources. Based on annual numbers of rigs in service and typical staffing patterns, annual mishap rates and fatality rates for rig types and mishap categories were computed. While the frequency of major mishaps has increased in recent years, the mishap rate per 100 rig-years of service has remained stable. The overall stability obscures the fact that jack-up rigs have had an increasing mishap rate while the rate for other rig types combined has gradually declined. Although the fatal mishap rate has also remained constant, the annual fatality rate per 100 000 full time equivalent (FTE) workers has risen sharply. This can be attributed to increasing numbers of lives lost in environmental mishaps while deaths from operational mishaps have declined. There were 344 fatalities during the 27-year period. Although an average of some 13 deaths per year worldwide appears minimal, the relatively small size of the workforce gives this number significance particularly when it is noted that 'occupational' fatalities, those occurring in the course of routine operations, are not included. The overall fatality rate secondary to major mishaps was 84.3 per 100 000 FTE worker-years.

Validation of an Adaptive Combustion Instability Control Method for Gas-Turbine Engines

NASA Technical Reports Server (NTRS)

Kopasakis, George; DeLaat, John C.; Chang, Clarence T.

2004-01-01

This paper describes ongoing testing of an adaptive control method to suppress high frequency thermo-acoustic instabilities like those found in lean-burning, low emission combustors that are being developed for future aircraft gas turbine engines. The method called Adaptive Sliding Phasor Averaged Control, was previously tested in an experimental rig designed to simulate a combustor with an instability of about 530 Hz. Results published earlier, and briefly presented here, demonstrated that this method was effective in suppressing the instability. Because this test rig did not exhibit a well pronounced instability, a question remained regarding the effectiveness of the control methodology when applied to a more coherent instability. To answer this question, a modified combustor rig was assembled at the NASA Glenn Research Center in Cleveland, Ohio. The modified rig exhibited a more coherent, higher amplitude instability, but at a lower frequency of about 315 Hz. Test results show that this control method successfully reduced the instability pressure of the lower frequency test rig. In addition, due to a certain phenomena discovered and reported earlier, the so called Intra-Harmonic Coupling, a dramatic suppression of the instability was achieved by focusing control on the second harmonic of the instability. These results and their implications are discussed, as well as a hypothesis describing the mechanism of intra-harmonic coupling.

Development of broad-spectrum human monoclonal antibodies for rabies post-exposure prophylaxis.

PubMed

De Benedictis, Paola; Minola, Andrea; Rota Nodari, Elena; Aiello, Roberta; Zecchin, Barbara; Salomoni, Angela; Foglierini, Mathilde; Agatic, Gloria; Vanzetta, Fabrizia; Lavenir, Rachel; Lepelletier, Anthony; Bentley, Emma; Weiss, Robin; Cattoli, Giovanni; Capua, Ilaria; Sallusto, Federica; Wright, Edward; Lanzavecchia, Antonio; Bourhy, Hervé; Corti, Davide

2016-04-01

Currently available rabies post-exposure prophylaxis (PEP) for use in humans includes equine or human rabies immunoglobulins (RIG). The replacement of RIG with an equally or more potent and safer product is strongly encouraged due to the high costs and limited availability of existing RIG. In this study, we identified two broadly neutralizing human monoclonal antibodies that represent a valid and affordable alternative to RIG in rabies PEP. Memory B cells from four selected vaccinated donors were immortalized and monoclonal antibodies were tested for neutralizing activity and epitope specificity. Two antibodies, identified as RVC20 and RVC58 (binding to antigenic site I and III, respectively), were selected for their potency and broad-spectrum reactivity. In vitro, RVC20 and RVC58 were able to neutralize all 35 rabies virus (RABV) and 25 non-RABV lyssaviruses. They showed higher potency and breath compared to antibodies under clinical development (namely CR57, CR4098, and RAB1) and commercially available human RIG. In vivo, the RVC20-RVC58 cocktail protected Syrian hamsters from a lethal RABV challenge and did not affect the endogenous hamster post-vaccination antibody response. © 2016 Humabs BioMed SA Published under the terms of the CC BY 4.0 license.