Role of Semaphorin 7a signaling in TGF-β1 induced lung fibrosis and scleroderma-related interstitial lung disease

PubMed Central

Gan, Ye; Reilkoff, Ronald; Peng, Xueyan; Russell, Thomas; Chen, Qingsheng; Mathai, Susan K.; Homer, Robert; Gulati, Mridu; Siner, Jonathan; Elias, Jack; Bucala, Richard; Herzog, Erica

2012-01-01

Objective Semaphorin (Sema) 7a regulates TGF- β1 induced fibrosis. Using a murine model of pulmonary fibrosis in which an inducible, bioactive form of the human TGF- β1 gene is overexpressed in the lung, we tested the hypothesis that Sema-7a exerts its pro-fibrotic effects in part by promoting the tissue accumulation of CD45+ fibrocytes. Methods Fibrosis and fibrocytes were evaluated in TGF- β1 transgenic mice in which the Sema-7a locus had been disrupted. The effect of replacement or deletion of Sema-7a on bone marrow derived cells was ascertained using bone marrow transplantation. The role of the Sema-7a receptor β1 integrin was assessed using neutralizing antibodies. The applicability of these findings to TGF-β1-driven fibrosis in humans was examined in patients with scleroderma-related interstitial lung disease. Results The appearance of fibrocytes in the lungs in TGF- β1 transgenic mice requires Sema-7a. Replacement of Sema-7a in bone marrow derived cells restores lung fibrosis and fibrocytes. Immunoneutralization of β1 integrin reduces pulmonary fibrocytes and fibrosis. Peripheral blood mononuclear cells from patients with scleroderma-related interstitial lung disease show increased mRNA for Sema-7a and the β1 integrin, with Sema-7a located on collagen producing fibrocytes and CD19+ lymphocytes. Peripheral blood fibrocyte outgrowth is enhanced in these patients. Stimulation of normal human peripheral blood mononuclear cells with recombinant Sema-7a enhances fibrocyte differentiation; these effects are attenuated by β1 integrin neutralization. Conclusion Interventions that reduce Sema-7a expression or prevent the Sema-7a - β1 integrin interaction may be ameliorative in TGF- β1-driven or fibrocyte-associated autoimmune fibroses. PMID:21484765

The axonal guidance cue semaphorin 3C contributes to alveolar growth and repair.

PubMed

Vadivel, Arul; Alphonse, Rajesh S; Collins, Jennifer J P; van Haaften, Tim; O'Reilly, Megan; Eaton, Farah; Thébaud, Bernard

2013-01-01

Lung diseases characterized by alveolar damage such as bronchopulmonary dysplasia (BPD) in premature infants and emphysema lack efficient treatments. Understanding the mechanisms contributing to normal and impaired alveolar growth and repair may identify new therapeutic targets for these lung diseases. Axonal guidance cues are molecules that guide the outgrowth of axons. Amongst these axonal guidance cues, members of the Semaphorin family, in particular Semaphorin 3C (Sema3C), contribute to early lung branching morphogenesis. The role of Sema3C during alveolar growth and repair is unknown. We hypothesized that Sema3C promotes alveolar development and repair. In vivo Sema3C knock down using intranasal siRNA during the postnatal stage of alveolar development in rats caused significant air space enlargement reminiscent of BPD. Sema3C knock down was associated with increased TLR3 expression and lung inflammatory cells influx. In a model of O2-induced arrested alveolar growth in newborn rats mimicking BPD, air space enlargement was associated with decreased lung Sema3C mRNA expression. In vitro, Sema3C treatment preserved alveolar epithelial cell viability in hyperoxia and accelerated alveolar epithelial cell wound healing. Sema3C preserved lung microvascular endothelial cell vascular network formation in vitro under hyperoxic conditions. In vivo, Sema3C treatment of hyperoxic rats decreased lung neutrophil influx and preserved alveolar and lung vascular growth. Sema3C also preserved lung plexinA2 and Sema3C expression, alveolar epithelial cell proliferation and decreased lung apoptosis. In conclusion, the axonal guidance cue Sema3C promotes normal alveolar growth and may be worthwhile further investigating as a potential therapeutic target for lung repair.

Cationic Peptides and Peptidomimetics Bind Glycosaminoglycans as Potential Sema3A Pathway Inhibitors

PubMed Central

Corredor, Miriam; Bonet, Roman; Moure, Alejandra; Domingo, Cecilia; Bujons, Jordi; Alfonso, Ignacio; Pérez, Yolanda; Messeguer, Àngel

2016-01-01

Semaphorin3A (Sema3A) is a vertebrate-secreted protein that was initially characterized as a repulsive-guidance cue. Semaphorins have crucial roles in several diseases; therefore, the development of Sema3A inhibitors is of therapeutic interest. Sema3A interacts with glycosaminoglycans (GAGs), presumably through its C-terminal basic region. We used different biophysical techniques (i.e., NMR, surface plasmon resonance, isothermal titration calorimetry, fluorescence, and UV-visible spectroscopy) to characterize the binding of two Sema3A C-terminus-derived basic peptides (FS2 and NFS3) to heparin and chondroitin sulfate A. We found that these peptides bind to both GAGs with affinities in the low-micromolar range. On the other hand, a peptoid named SICHI (semaphorin-induced chemorepulsion inhibitor), which is positively charged at physiological pH, was first identified by our group as being able to block Sema3A chemorepulsion and growth-cone collapse in axons at the extracellular level. To elucidate the direct target for the reported SICHI inhibitory effect in the Sema3A signaling pathway, we looked first to the protein-protein interaction between secreted Sema3A and the Nrp1 receptor. However, our results show that SICHI does not bind directly to the Sema3A sema domain or to Nrp1 extracellular domains. We evaluated a new, to our knowledge, hypothesis, according to which SICHI binds to GAGs, thereby perturbing the Sema3A-GAG interaction. By using the above-mentioned techniques, we observed that SICHI binds to GAGs and competes with Sema3A C-terminus-derived basic peptides for binding to GAGs. These data support the ability of SICHI to block the biologically relevant interaction between Sema3A and GAGs, thus revealing SICHI as a new, to our knowledge, class of inhibitors that target the GAG-protein interaction. PMID:27028639

Are Sema5a mutant mice a good model of autism? A behavioral analysis of sensory systems, emotionality and cognition

PubMed Central

Gunn, Rhian K.; Huentelman, Matthew J.; Brown, Richard E.

2011-01-01

Semaphorin 5A (Sema5A) expression is reduced in the brain of individuals with autism, thus mice with reduced Sema5A levels may serve as a model of this neurodevelopmental disorder. We tested male and female Sema5a knockout mice (B6.129P2SEMA5A/J) and C57BL/6J controls for emotionality, visual ability, prepulse inhibition, motor learning and cognition. Overall, there were only two genotype differences in emotionality: Sema5a mutant mice had more stretch-attend postures in the elevated plus-maze and more defecations in the open field. All mice could see, but Sema5a mice had better visual ability than C57BL/6J mice. There were no genotype differences in sensory-motor gating. Sema5a mice showed higher levels of activity in the elevated plus-maze and light/dark transition box, and there were sex by genotype differences in the Rotarod, suggesting a sex difference in balance and coordination differentially affected by Sema5a. There were no genotype effects on cognition: Sema5a mice did not differ from C57BL/6J in the Morris water maze, set-shifting or cued and contextual fear conditioning. In the social recognition test, all mice preferred social stimuli, but there was no preference for social novelty, thus the Sema5A mice do not have a deficit in social behavior. Overall, there were a number of sex differences, with females showing greater activity and males performing better in tests of spatial learning and memory, but no deficits in the behavior of Sema5A mice. We conclude that the Sema5a mice do not meet the behavioral criteria for a mouse model of autism. PMID:21777623

Excess Podocyte Semaphorin-3A Leads to Glomerular Disease Involving PlexinA1–Nephrin Interaction

PubMed Central

Reidy, Kimberly J.; Aggarwal, Pardeep K.; Jimenez, Juan J.; Thomas, David B.; Veron, Delma; Tufro, Alda

2014-01-01

Semaphorin-3A (Sema3a), a guidance protein secreted by podocytes, is essential for normal kidney patterning and glomerular filtration barrier development. Here, we report that podocyte-specific Sema3a gain-of-function in adult mice leads to proteinuric glomerular disease involving the three layers of the glomerular filtration barrier. Reversibility of the glomerular phenotype upon removal of the transgene induction provided proof-of-principle of the cause-and-effect relationship between podocyte Sema3a excess and glomerular disease. Mechanistically, excess Sema3a induces dysregulation of nephrin, matrix metalloproteinase 9, and αvβ3 integrin in vivo. Sema3a cell-autonomously disrupts podocyte shape. We identified a novel direct interaction between the Sema3a signaling receptor plexinA1 and nephrin, linking extracellular Sema3a signals to the slit-diaphragm signaling complex. We conclude that Sema3a functions as an extracellular negative regulator of the structure and function of the glomerular filtration barrier in the adult kidney. Our findings demonstrate a crosstalk between Sema3a and nephrin signaling pathways that is functionally relevant both in vivo and in vitro. PMID:23954273

Establishing Integrated Tech Prep Programs in Urban Schools: Plans Developed at the NCRVE 1993 National Institute.

ERIC Educational Resources Information Center

National Center for Research in Vocational Education, Berkeley, CA.

This document outlines the purposes and main activities of a 2-week national institute held in July 1993 in Berkeley, California. Ten teams of vocational and academic educators (125 high school and community college teachers, counselors, and administrators) from 10 major metropolitan areas were selected through a competitive application process to…

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yamada, Daisuke; Kawahara, Kohichi; Maeda, Takehiko, E-mail: maeda@nupals.ac.jp

Aberration of signaling pathways by genetic mutations or alterations in the surrounding tissue environments can result in tumor development or metastasis. However, signaling molecules responsible for these processes have not been completely elucidated. Here, we used mouse Lewis lung carcinoma cells (LLC) to explore the mechanism by which the oncogenic activity of Semaphorin3A (Sema3A) signaling is regulated. Sema3A knockdown by shRNA did not affect apoptosis, but decreased cell proliferation in LLCs; both the mammalian target of rapamycin complex 1 (mTORC1) level and glycolytic activity were also decreased. In addition, Sema3A knockdown sensitized cells to inhibition of oxidative phosphorylation by oligomycin,more » but conferred resistance to decreased cell viability induced by glucose starvation. Furthermore, recombinant SEMA3A rescued the attenuation of cell proliferation and glycolytic activity in LLCs after Sema3A knockdown, whereas mTORC1 inhibition by rapamycin completely counteracted this effect. These results demonstrate that Sema3A signaling exerts its oncogenic effect by promoting an mTORC1-mediated metabolic shift from oxidative phosphorylation to aerobic glycolysis. -- Highlights: •Sema3A knockdown decreased proliferation of Lewis lung carcinoma cells (LLCs). •Sema3A knockdown decreased mTORC1 levels and glycolytic activity in LLCs. •Sema3A knockdown sensitized cells to inhibition of oxidative phosphorylation. •Sema3A promotes shift from oxidative phosphorylation to aerobic glycolysis via mTORC1.« less

Comprehensive behavioral phenotyping of a new Semaphorin 3 F mutant mouse.

PubMed

Matsuda, Ikuo; Shoji, Hirotaka; Yamasaki, Nobuyuki; Miyakawa, Tsuyoshi; Aiba, Atsu

2016-02-09

Semaphorin 3 F (Sema3F) is a secreted type of the Semaphorin family of axon guidance molecules. Sema3F and its receptor neuropilin-2 (Npn-2) are expressed in a mutually exclusive manner in the embryonic mouse brain regions including olfactory bulb, hippocampus, and cerebral cortex. Sema3F is thought to have physiological functions in the formation of neuronal circuitry and its refinement. However, functional roles of Sema3F in the brain remain to be clarified. Here, we examined behavioral effects of Sema3F deficiency through a comprehensive behavioral test battery in Sema3F knockout (KO) male mice to understand the possible functions of Sema3F in the brain. Male Sema3F KO and wild-type (WT) control mice were subjected to a battery of behavioral tests, including neurological screen, rotarod, hot plate, prepulse inhibition, light/dark transition, open field, elevated plus maze, social interaction, Porsolt forced swim, tail suspension, Barnes maze, and fear conditioning tests. In the open field test, Sema3F KO mice traveled shorter distance and spent less time in the center of the field than WT controls during the early testing period. In the light/dark transition test, Sema3F KO mice also exhibited decreased distance traveled, fewer number of transitions, and longer latency to enter the light chamber compared with WT mice. In addition, Sema3F KO mice traveled shorter distance than WT mice in the elevated plus maze test, although there were no differences between genotypes in open arm entries and time spent in open arms. Similarly, Sema3F KO mice showed decreased distance traveled in the social interaction test. Sema3F KO mice displayed reduced immobility in the Porsolt forced swim test whereas there was no difference in immobility between genotypes in the tail suspension test. In the fear conditioning test, Sema3F KO mice exhibited increased freezing behavior when exposed to a conditioning context and an altered context in absence of a conditioned stimulus. In the tests for assessing motor function, pain sensitivity, startle response to an acoustic stimulus, sensorimotor gating, or spatial reference memory, there were no significant behavioral differences between Sema3F KO and WT mice. These results suggest that Sema3F deficiency induces decreased locomotor activity and possibly abnormal anxiety-related behaviors and also enhances contextual memory and generalized fear in mice. Thus, our findings suggest that Sema3F plays important roles in the development of neuronal circuitry underlying the regulation of some aspects of anxiety and fear responses.

Semaphorin 3A

PubMed Central

Xu, Ren

2014-01-01

Semaphorin 3A (Sema3A) is a protein identified originally as a diffusible axonal chemorepellent. Sema3A has multifunctional roles in embryonic development, immune regulation, vascularization, and oncogenesis. Bone remodeling consists of two phases: the removal of mineralized bone by osteoclasts and the formation of new bone by osteoblasts, and plays an essential role in skeletal diseases such as osteoporosis. Recent studies have shown that Sema3A is implicated in the regulation of osteoblastgenesis and osteoclastgenesis. Moreover, low bone mass in mice with specific knockout of Sema3A in the neurons indicates that Sema3A regulates bone remodeling indirectly. This review highlights recent advances on our understanding of the role of sema3A as a new player in the regulation of bone remodeling and proposes the potential of sema3A in the diagnosis and therapy of bone diseases. PMID:24589620

Astronomer to Data Scientist

NASA Astrophysics Data System (ADS)

Kirkpatrick, Jessica

2015-01-01

Jessica Kirkpatrick received her PhD in Astrophysics from Berkeley in 2012. After an exhaustive job search within academia and beyond, she accepted a job as a data scientist / analyst for the social network Yammer (acquired by Microsoft) and is now the Director of Data Science for Education Company InstaEDU. Now instead of spending her days finding patterns in the large scale structure of galaxies, she finds patterns in the behaviors of people. She'll talk about her transition from astrophysics to tech, compare and contrast the two fields, and give tips about how to land a tech job, and discuss useful tools which helped her with her transition.

Sema-1a Reverse Signaling Promotes Midline Crossing in Response to Secreted Semaphorins.

PubMed

Hernandez-Fleming, Melissa; Rohrbach, Ethan W; Bashaw, Greg J

2017-01-03

Commissural axons must cross the midline to form functional midline circuits. In the invertebrate nerve cord and vertebrate spinal cord, midline crossing is mediated in part by Netrin-dependent chemoattraction. Loss of crossing, however, is incomplete in mutants for Netrin or its receptor Frazzled/DCC, suggesting the existence of additional pathways. We identified the transmembrane Semaphorin, Sema-1a, as an important regulator of midline crossing in the Drosophila CNS. We show that in response to the secreted Semaphorins Sema-2a and Sema-2b, Sema-1a functions as a receptor to promote crossing independently of Netrin. In contrast to other examples of reverse signaling where Sema1a triggers repulsion through activation of Rho in response to Plexin binding, in commissural neurons Sema-1a acts independently of Plexins to inhibit Rho to promote attraction to the midline. These findings suggest that Sema-1a reverse signaling can elicit distinct axonal responses depending on differential engagement of distinct ligands and signaling effectors. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib

PubMed Central

Yang, Lida; Kaur, Harmandeep; Pestel, Jenny; Looso, Mario; Nolte, Hendrik; Krishnan, Ramesh K.; Bünemann, Moritz; Offermanns, Stefan; Swiercz, Jakub M.

2017-01-01

Semaphorins comprise a large family of ligands that regulate key cellular functions through their receptors, plexins. In this study, we show that the transmembrane semaphorin 4A (Sema4A) can also function as a receptor, rather than a ligand, and transduce signals triggered by the binding of Plexin-B1 through reverse signaling. Functionally, reverse Sema4A signaling regulates the migration of various cancer cells as well as dendritic cells. By combining mass spectrometry analysis with small interfering RNA screening, we identify the polarity protein Scrib as a downstream effector of Sema4A. We further show that binding of Plexin-B1 to Sema4A promotes the interaction of Sema4A with Scrib, thereby removing Scrib from its complex with the Rac/Cdc42 exchange factor βPIX and decreasing the activity of the small guanosine triphosphatase Rac1 and Cdc42. Our data unravel a role for Plexin-B1 as a ligand and Sema4A as a receptor and characterize a reverse signaling pathway downstream of Sema4A, which controls cell migration. PMID:28007914

Semaphorin 3C Released from a Biocompatible Hydrogel Guides and Promotes Axonal Growth of Rodent and Human Dopaminergic Neurons

PubMed Central

Carballo-Molina, Oscar A.; Sánchez-Navarro, Andrea; López-Ornelas, Adolfo; Lara-Rodarte, Rolando; Salazar, Patricia; Campos-Romo, Aurelio; Ramos-Mejía, Verónica

2016-01-01

Cell therapy in experimental models of Parkinson's disease replaces the lost dopamine neurons (DAN), but we still need improved methods to guide dopaminergic axons (DAx) of grafted neurons to make proper connections. The protein Semaphorin 3C (Sema3C) attracts DAN axons and enhances their growth. In this work, we show that the hydrogel PuraMatrix, a self-assembling peptide-based matrix, incorporates Sema3C and releases it steadily during 4 weeks. We also tested if hydrogel-delivered Sema3C attracts DAx using a system of rat midbrain explants embedded in collagen gels. We show that Sema3C released by this hydrogel attracts DAx, in a similar way to pretectum, which is known to attract growing DAN axons. We assessed the effect of Sema3C on the growth of DAx using microfluidic devices. DAN from rat midbrain or those differentiated from human embryonic stem cells showed enhanced axonal extension when exposed to hydrogel-released Sema3C, similar to soluble Sema3C. Notably, DAN of human origin express the cognate Sema3C receptors, Neuropilin1 and Neuropilin2. These results show that PuraMatrix is able to incorporate and release Sema3C, and such delivery guides and promotes the axonal growth of DAN. This biocompatible hydrogel might be useful as a Sema3C carrier for in vivo studies in parkinsonian animal models. PMID:27174503

Autocrine Semaphorin3A signaling is essential for the maintenance of stem-like cells in lung cancer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yamada, Daisuke; Takahashi, Kensuke; Kawahara, Kohichi

Cancer stem-like cells (CSCs) exist in tumor tissues composed of heterogeneous cell population and are characterized by their self-renewal capacity and tumorigenicity. Many studies demonstrate that eradication of CSCs prevents development and recurrences of tumor; yet, molecules critical for the maintenance of CSCs have not been completely understood. We previously reported that Semaphorin3A (Sema3a) knockdown suppressed the tumorigenicity and proliferative capacity of Lewis lung carcinoma (LLC) cells. Therefore, we identified Sema3a as an essential factor for the establishment or maintenance of CSCs derived from LLC (LLC-stem cell). shRNA against Sema3a was introduced into LLC cells to establish a LLC-stem cellmore » line and its effects on tumorigenesis, sphere formation, and mTORC1 activity were tested. Sema3a knockdown completely abolished tumorigenicity and the sphere-formation and self-renewal ability of LLC-stem cells. The Sema3a knockdown was also associated with decreased expression of mRNA for stem cell markers. The self-renewal ability abolished by Sema3a knockdown could not be recovered by exogenous addition of recombinant SEMA3A. In addition, the activity of mammalian target of rapamycin complex 1 (mTORC1) and the expression of its substrate p70S6K1 were also decreased. These results demonstrate that Sema3a is a potential therapeutic target in eradication of CSCs. - Highlights: • Sema3a enhances tumorigenic capacity of cancer stem-like cells. • Sema3a is essential for the maintenance of cancer stem-like cells. • Sema3a can be a therapeutic target to eradicate cancer stem-like cells.« less

Semaphorin 4D in human head and neck cancer tissue and peripheral blood: A dense fibrotic peri-tumoral stromal phenotype

PubMed Central

Derakhshandeh, Roshanak; Sanadhya, Sonia; Lee Han, Kyu; Chen, Haiyan; Goloubeva, Olga; Webb, Tonya J.; Younis, Rania H.

2018-01-01

The search for stromal biomarkers in carcinoma patients is a challenge in the field. Semaphorin 4D (Sema4D), known for its various developmental, physiological and pathological effects, plays a role in pro and anti-inflammatory responses. It is expressed in many epithelial tumors including head and neck squamous cell carcinoma (HNSCC). Recently, we found that HNSCC-associated Sema4D modulates an immune-suppressive, tumor-permissible environment by inducing the expansion of myeloid derived suppressor cells. The purpose of this study was to determine the value of Sema4D as a biomarker for the peri-tumoral stromal phenotype in human HNSCC. Our data showed Sema4D+ve/high tumor cells in 34% of the studied cohort with positive correlation to Stage III (p=0.0001). Sema4D+ve/high tumor cells correlated directly with dense fibrotic peri-tumoral stroma (p=0.0001) and inversely with infiltrate of Sema4D+ve/high tumor-associated inflammatory cells (TAIs) (p=0.01). Most of the Sema4D+ve/high TAIs were co-positive for the macrophage biomarker CD163. Knockdown of Sema4D in WSU-HN6 cells inhibited collagen production by fibroblasts, and decreased activated TGF-β1 levels in culture medium of HNSCC cell lines. In a stratification model of HNSCC using combined Sema4D and the programmed death ligand 1 (PDL-1), Sema4D+ve/high tumor cells represented a phenotype distinct from the PDL-1 positive tumors. Finally,Sema4D was detected in plasma of HNC patients at significantly higher levels (115.44, ± 39.37) compared to healthy donors (38.60± 12.73) (p <0.0001). In conclusion, we present a novel HNSCC tumor stratification model, based on the expression of the biomarker Sema4D. This model opens new avenues to novel targeted therapeutic strategies. PMID:29541402

Semaphorin 3A is expressed in human osteoarthritic cartilage and antagonizes vascular endothelial growth factor 165-promoted chondrocyte migration: an implication for chondrocyte cloning.

PubMed

Okubo, Masashi; Kimura, Tokuhiro; Fujita, Yoshinari; Mochizuki, Satsuki; Niki, Yasuo; Enomoto, Hiroyuki; Suda, Yasunori; Toyama, Yoshiaki; Okada, Yasunori

2011-10-01

Vascular endothelial growth factor 165 (VEGF165) and its receptors, including neuropilin 1 (NRP-1), are overexpressed in human osteoarthritic (OA) articular cartilage, although their functional roles in the cartilage are not fully understood. An axon-guidance molecule, semaphorin 3A (Sema3A), which binds to NRP-1, acts as an antagonist of VEGF signaling in endothelial cells. The aim of this study was to examine the expression of Sema3A and the functions of the VEGF165/Sema3A/NRP-1 axis in OA cartilage. The expression of Sema3A in OA and normal cartilage samples was examined by real-time polymerase chain reaction and immunohistochemical analyses. Functional analyses of VEGF165 and Sema3A were carried out using OA chondrocytes in culture. The migration activity of chondrocytes was examined in a monolayer wound assay. The effects of Sema3A on VEGF165-induced up-regulation of matrix metalloproteinases (MMPs) and intracellular signaling were also studied in cultured chondrocytes. Sema3A expression was significantly elevated in OA cartilage as compared to normal cartilage. Sema3A immunoreactivity directly correlated with the Mankin score and with chondrocyte cloning. VEGF165 promoted the migration of chondrocytes, and this activity was suppressed by VEGF receptor 2 tyrosine kinase inhibitors. Sema3A antagonized the chondrocyte migration promoted by VEGF165, and the activity was blocked by a selective inhibitor of, or small interfering RNA for, Sema3A. VEGF165-induced overexpression of MMPs and phosphorylation of ERK and focal adhesion kinase in chondrocytes were inhibited by Sema3A. Our findings provide the first evidence that Sema3A is overexpressed, with a direct correlation with cloning, in OA cartilage and that it suppresses the VEGF165-promoted migration of chondrocytes. Our findings also suggest that Sema3A plays a role in chondrocyte cloning through inhibition of cell migration in OA cartilage. Copyright © 2011 by the American College of Rheumatology.

Estrogen-Dependent Proteolytic Cleavage of Semaphorin 4D and Plexin-B1 Enhances Semaphorin 4D-Induced Apoptosis during Postnatal Vaginal Remodeling in Pubescent Mice

PubMed Central

Yoshida, Kenji; Ueyama, Takashi; Miyajima, Masayasu; Negishi, Takayuki; Kawasaki, Takahiko; Takamatsu, Hyota; Kikutani, Hitoshi; Kumanogoh, Atsushi; Yukawa, Kazunori

2014-01-01

Around the fifth week after birth, the vaginal cavity in female mouse pups opens to the overlaying skin. This postnatal tissue remodeling of the genital tract occurs during puberty, and it largely depends upon hormonally induced apoptosis that mainly occurs in the epithelium at the lower part of the mouse vaginal cavity. Previously, we showed that most BALB/c mice lacking the class IV Semaphorin (Sema4D) develop imperforate vagina and hydrometrocolpos; therefore, we reasoned that the absence of Sema4D-induced apoptosis in vaginal epithelial cells may cause the imperforate vagina. Sema4D signals via the Plexin-B1 receptor; nevertheless detailed mechanisms mediating this hormonally triggered apoptosis are not fully documented. To investigate the estrogen-dependent control of Sema4D signaling during the apoptosis responsible for mouse vaginal opening, we examined structural and functional modulation of Sema4D, Plexin-B1, and signaling molecules by analyzing both wild-type and Sema4D−/− mice with or without ovariectomy. Both the release of soluble Sema4D and the conversion of Plexin-B1 by proteolytic processing in vaginal tissue peaked 5 weeks after birth of wild-type BALB/c mice at the time of vaginal opening. Estrogen supplementation of ovariectomized wild-type mice revealed that both the release of soluble Sema4D and the conversion of Plexin-B1 into an active form were estrogen-dependent and concordant with apoptosis. Estrogen supplementation of ovariectomized Sema4D−/− mice did not induce massive vaginal apoptosis in 5-week-old mice; therefore, Sema4D may be an essential apoptosis-inducing ligand that acts downstream of estrogen action in vaginal epithelium during this postnatal tissue remodeling. Analysis of ovariectomized mice also indicated that Sema4D contributed to estrogen-dependent dephosphorylation of Akt and ERK at the time of vaginal opening. Based on our results, we propose that apoptosis in vaginal epithelium during postnatal vaginal opening is induced by enhanced Sema4D signaling that is caused by estrogen-dependent structural changes of Sema4D and Plexin-B1. PMID:24841081

DOE Office of Scientific and Technical Information (OSTI.GOV)

Morishige, Naoyuki; Ko, Ji-Ae, E-mail: jiae0831@yamaguchi-u.ac.jp; Morita, Yukiko

The neural guidance protein semaphorin 3A (Sema3A) is expressed in corneal epithelial cells of the adult rat. We have now further investigated the localization of Sema3A in the normal rat corneal epithelium as well as changes in its expression pattern during wound healing after central corneal epithelial debridement. The expression pattern of Sema3A was compared with that of the tight-junction protein zonula occludens-1 (ZO-1), the gap-junction protein connexin43 (Cx43), or the cell proliferation marker Ki67. Immunofluorescence analysis revealed that Sema3A was present predominantly in the membrane of basal and wing cells of the intact corneal epithelium. The expression of Sema3Amore » at the basal side of basal cells was increased in the peripheral epithelium compared with that in the central region. Sema3A was detected in all layers at the leading edge of the migrating corneal epithelium at 6 h after central epithelial debridement. The expression of Sema3A was markedly up-regulated in the basal and lateral membranes of columnar basal cells apparent in the thickened, newly healed epithelium at 1 day after debridement, but it had largely returned to the normal pattern at 3 days after debridement. The expression of ZO-1 was restricted to superficial epithelial cells and remained mostly unchanged during the wound healing process. The expression of Cx43 in basal cells was down-regulated at the leading edge of the migrating epithelium but was stable in the remaining portion of the epithelium. Ki67 was not detected in basal cells of the central epithelium at 1 day after epithelial debridement, when Sema3A was prominently expressed. Immunoblot analysis showed that the abundance of Sema3A in the central cornea was increased 1 day after epithelial debridement, whereas that of ZO-1 or Cx43 remained largely unchanged. This increase in Sema3A expression was accompanied by up-regulation of the Sema3A coreceptor neuropilin-1. Our observations have thus shown that the expression of Sema3A is increased markedly in basal cells of the newly healed corneal epithelium, and that this up-regulation of Sema3A is not associated with cell proliferation. They further suggest that Sema3A might play a role in the regulation of corneal epithelial wound healing.« less

Mutation screening of SEMA3A and SEMA7A in patients with congenital hypogonadotropic hypogonadism.

PubMed

Känsäkoski, Johanna; Fagerholm, Rainer; Laitinen, Eeva-Maria; Vaaralahti, Kirsi; Hackman, Peter; Pitteloud, Nelly; Raivio, Taneli; Tommiska, Johanna

2014-05-01

Congenital hypogonadotropic hypogonadism (HH), a rare disorder characterized by absent, partial, or delayed puberty, can be caused by the lack or deficient number of hypothalamic gonadotropin-releasing hormone (GnRH) neurons. SEMA3A was recently implicated in the etiology of the disorder, and Sema7A-deficient mice have a reduced number of GnRH neurons in their brains. SEMA3A and SEMA7A were screened by Sanger sequencing in altogether 50 Finnish HH patients (34 with Kallmann syndrome (KS; HH with hyposmia/anosmia) and 16 with normosmic HH (nHH)). In 20 patients, mutation(s) had already been found in genes known to be implicated in congenital HH. Three heterozygous variants (c.458A>G (p.Asn153Ser), c.1253A>G (p.Asn418Ser), and c.1303G>A (p.Val435Ile)) were found in SEMA3A in three KS patients, two of which also had a mutation in FGFR1. Two rare heterozygous variants (c.442C>T (p.Arg148Trp) and c.1421G>A (p.Arg474Gln)) in SEMA7A were found in one male nHH patient with a previously identified KISS1R nonsense variant and one male KS patient with a previously identified mutation in KAL1, respectively. Our results suggest that heterozygous missense variants in SEMA3A and SEMA7A may modify the phenotype of KS but most likely are not alone sufficient to cause the disorder.

Endogenous axon guiding chemorepulsant semaphorin-3F inhibits the growth and metastasis of colorectal carcinoma.

PubMed

Wu, Feng; Zhou, Qi; Yang, Jing; Duan, Guang-jie; Ou, Juan-juan; Zhang, Rong; Pan, Feng; Peng, Qiu-ping; Tan, Hong; Ping, Yi-fang; Cui, You-hong; Qian, Cheng; Yan, Xiao-chu; Bian, Xiu-wu

2011-05-01

To elucidate the role of Semaphorin-3F (SEMA3F), originally described as an axon guiding chemorepulsant implicated in nerve development, in the progression of colorectal carcinoma. SEMA3F and its receptor NRP2 were examined in 72 cases of human colorectal carcinoma specimens and cell lines LoVo, SW480, and SW620 with immunohistochemistry and Western blotting. SEMA3F mRNA expression in the frozen tissue specimens and cell lines was examined with quantitative reverse transcriptase-PCR. Confocal laser scanning microscopy was used for detection of cellular localization of the proteins by immunofluorescent staining. MTT assay, flow cytometry, cell adhesion and migration, and xenografts were used to evaluate biological significance of SEMA3F. SEMA3F was significantly reduced in colorectal carcinoma tissues and cell lines. Overexpression of SEMA3F resulted in reduced proliferation, adhesion to fibronectin, and migratory capability as well as reduced S-phase population and integrin αvβ3 expression of SW480 colon cancer cells. In addition, SEMA3F-overexpressing cells exhibited diminished tumorigenesis when transplanted orthotopically in nude mice and reduced liver metastases. Moreover, transfection of siRNA targeting SEMA3F in colon cancer cells increased their tumorigenicity in vivo. Endogenous SEMA3F acts as a suppressor of the growth and metastasis of human colorectal cancer cells. ©2011 AACR.

A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib.

PubMed

Sun, Tianliang; Yang, Lida; Kaur, Harmandeep; Pestel, Jenny; Looso, Mario; Nolte, Hendrik; Krasel, Cornelius; Heil, Daniel; Krishnan, Ramesh K; Santoni, Marie-Josée; Borg, Jean-Paul; Bünemann, Moritz; Offermanns, Stefan; Swiercz, Jakub M; Worzfeld, Thomas

2017-01-02

Semaphorins comprise a large family of ligands that regulate key cellular functions through their receptors, plexins. In this study, we show that the transmembrane semaphorin 4A (Sema4A) can also function as a receptor, rather than a ligand, and transduce signals triggered by the binding of Plexin-B1 through reverse signaling. Functionally, reverse Sema4A signaling regulates the migration of various cancer cells as well as dendritic cells. By combining mass spectrometry analysis with small interfering RNA screening, we identify the polarity protein Scrib as a downstream effector of Sema4A. We further show that binding of Plexin-B1 to Sema4A promotes the interaction of Sema4A with Scrib, thereby removing Scrib from its complex with the Rac/Cdc42 exchange factor βPIX and decreasing the activity of the small guanosine triphosphatase Rac1 and Cdc42. Our data unravel a role for Plexin-B1 as a ligand and Sema4A as a receptor and characterize a reverse signaling pathway downstream of Sema4A, which controls cell migration. © 2017 Sun et al.

Anti-Semaphorin 3A neutralization monoclonal antibody prevents sepsis development in lipopolysaccharide-treated mice.

PubMed

Yamashita, Naoya; Jitsuki-Takahashi, Aoi; Ogawara, Miyuki; Ohkubo, Wataru; Araki, Tomomi; Hotta, Chie; Tamura, Tomohiko; Hashimoto, Shu-ichi; Yabuki, Takashi; Tsuji, Toru; Sasakura, Yukie; Okumura, Hiromi; Takaiwa, Aki; Koyama, Chika; Murakami, Koji; Goshima, Yoshio

2015-09-01

Semaphorin 3A (Sema3A), originally identified as a potent growth cone collapsing factor in developing sensory neurons, is now recognized as a key player in immune, cardiovascular, bone metabolism and neurological systems. Here we established an anti-Sema3A monoclonal antibody that neutralizes the effects of Sema3A both in vitro and in vivo. The anti-Sema3A neutralization chick IgM antibodies were screened by combining an autonomously diversifying library selection system and an in vitro growth cone collapse assay. We further developed function-blocking chick-mouse chimeric and humanized anti-Sema3A antibodies. We found that our anti-Sema3A antibodies were effective for improving the survival rate in lipopolysaccharide-induced sepsis in mice. Our antibody is a potential therapeutic agent that may prevent the onset of or alleviate symptoms of human diseases associated with Sema3A. © The Japanese Society for Immunology. 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

DRG axon elongation and growth cone collapse rate induced by Sema3A are differently dependent on NGF concentration.

PubMed

Kaselis, Andrius; Treinys, Rimantas; Vosyliūtė, Rūta; Šatkauskas, Saulius

2014-03-01

Regeneration of embryonic and adult dorsal root ganglion (DRG) sensory axons is highly impeded when they encounter neuronal growth cone-collapsing factor semaphorin3A (Sema3A). On the other hand, increasing evidence shows that DRG axon's regeneration can be stimulated by nerve growth factor (NGF). In this study, we aimed to evaluate whether increased NGF concentrations can counterweight Sema3A-induced inhibitory responses in 15-day-old mouse embryo (E15) DRG axons. The DRG explants were grown in Neurobasal-based medium with different NGF concentrations ranging from 0 to 100 ng/mL and then treated with Sema3A at constant 10 ng/mL concentration. To evaluate interplay between NGF and Sema3A number of DRG axons, axon outgrowth distance and collapse rate were measured. We found that the increased NGF concentrations abolish Sema3A-induced inhibitory effect on axon outgrowth, while they have no effect on Sema3A-induced collapse rate.

[Expression and Clinical Significance of Semaphorin 3A in serum and mononuclear cells in patients with systemic lupus erythematosus].

PubMed

Gao, H; Ma, X X; Guo, Q; Zou, Y D; Zhong, Y C; Xie, L F; Shao, M; Zhang, X W

2017-02-07

Objective: To determine the expression of Sema3A in serum and peripheral blood mononuclear cells (PBMC) of patients with systemic lupus erythematosus (SLE), to analysis the correlation of Sema3A expression and SLE clinical manifestations and laboratory indexes, and to evaluate the diagnostic value of Sema3A in patients with SLE. Methods: The concentration of serum Sema3A was detected by enzyme-linked immuno sorbent assay (ELISA) in patients with SLE, healthy controls (HC) and diseases controls. In addition, the mRNA expression level of Sema3A was examined in PBMC by real-time polymerase chain reaction. The correlation of serum Sema3A level and clinical and laboratory features of SLE patients were analyzed. Unpaired t test, Kruskal-Wallis test, Mann-Whitney U test, χ(2) test, Pearson and Spearman correlation analysis were used to statistical analysis by using SPSS 13.0. Results: (1) Serum Sema3A concentration in patients with SLE was significantly lower than that in HC groups ( P <0.01). (2) Consistent with the serum level, the Sema3A mRNA level in SLE was lower than that in HC ( P =0.001). And the mRNA expression of Nrp-1 in SLE was also lower than that in HC ( P <0.01). (3) The serum Sema3A level in patients with SLE was positively correlated with haemoglobin (HGB) ( r =0.271, P <0.013), platelet (PLT) ( r =0.600, P <0.011), complement 3 (C3) ( r =0.234, P =0.0027) and complement 4 (C4) ( r =0.159, P =0.434) levels. Whereas, the expression of Sema3A was negatively correlated with SLE disease activity index (SLEDAI) ( r =-0.286, P =0.036). (4) Area under curve illustrated by ROC curve was 0.876 (95% CI: 0.846-0.906). The best cut-off value for the diagnosis of SLE was 6.31 μg/L, with the sensitivity of 80.6% and the specificity of 77.5%. The Youden index was 0.581. Above results indicated good validity of Sema3A as a diagnostic marker for SLE. (5) The HGB, PLT, C3 and C4 levels in the group of Sema3A- positiveSLE patients (≤6.31 μg/L) were lower than that in negative group (>6.31 μg/L), while CRP level and SLEDAI of positive group was higher than that in negative group( P <0.05). In addition, the positive rate of antinuclear antibodies ( P =0.046) and anticardiolipin antibody ( P =0.018) in the Sema3A-negative group were also significantly higher than that of negative group. Conclusions: Sema3A and Nrp-1 was both decreased in serum and PBMC of SLE patients, suggesting that the circulating expression of Sema3A and Nrp-1 was seriously defected in SLE. Circulating Sema3A was significantly correlated with disease activity and blood damage in patients with SLE. The result of ROC curve showed that Sema3A had the potential to be a new diagnostic biomarker in SLE.

Semaphorin 4D induces vaginal epithelial cell apoptosis to control mouse postnatal vaginal tissue remodeling.

PubMed

Ito, Takuji; Bai, Tao; Tanaka, Tetsuji; Yoshida, Kenji; Ueyama, Takashi; Miyajima, Masayasu; Negishi, Takayuki; Kawasaki, Takahiko; Takamatsu, Hyota; Kikutani, Hitoshi; Kumanogoh, Atsushi; Yukawa, Kazunori

2015-02-01

The opening of the mouse vaginal cavity to the skin is a postnatal tissue remodeling process that occurs at approximately five weeks of age for the completion of female genital tract maturation at puberty. The tissue remodeling process is primarily composed of a hormonally triggered apoptotic process predominantly occurring in the epithelium of the distal section of the vaginal cavity. However, the detailed mechanism underlying the apoptotic induction remains to be elucidated. In the present study, it was observed that the majority of BALB/c mice lacking the class 4 semaphorin, semaphorin 4D (Sema4D), developed imperforate vagina and hydrometrocolpos resulting in a perpetually unopened vaginal cavity regardless of a normal estrogen level comparable with that in wild‑type (WT) mice. Administration of β‑estradiol to infant Sema4D‑deficient (Sema4D‑/‑) mice did not induce precocious vaginal opening, which was observed in WT mice subjected to the same β‑estradiol administration, excluding the possibility that the closed vaginal phenotype was due to insufficient estrogen secretion at the time of vaginal opening. In order to assess the role of Sema4D in the postnatal vaginal tissue remodeling process, the expression of Sema4D and its receptor, plexin‑B1, was examined as well as the level of apoptosis in the vaginal epithelia of five‑week‑old WT and Sema4D‑/‑ mice. Immunohistochemical analyses confirmed the localization of Sema4D and plexin‑B1 in the mouse vaginal epithelia. Terminal deoxynucleotidyl transferase dUTP nick end labeling assay and immunohistochemistry detecting activated caspase‑3 revealed significantly fewer apoptotic cells in situ in the vaginal mucosa of five‑week‑old Sema4D‑/‑ mice compared with WT mice. The addition of recombinant Sema4D to Sema4D‑/‑ vaginal epithelial cells in culture significantly enhanced apoptosis of the vaginal epithelial cells, demonstrating the apoptosis‑inducing activity of Sema4D. The experimental reduction of plexin‑B1 expression in vaginal epithelial cells demonstrated the integral role of plexin‑B1 in Sema4D‑induced apoptotic cell death. These results suggest a non‑redundant role of Sema4D in the postnatal tissue remodeling process in five‑week‑old BALB/c mice, which involves the induction of vaginal epithelial cell apoptosis through Sema4D binding to plexin‑B1.

Semaphorin 4D induces vaginal epithelial cell apoptosis to control mouse postnatal vaginal tissue remodeling

PubMed Central

ITO, TAKUJI; BAI, TAO; TANAKA, TETSUJI; YOSHIDA, KENJI; UEYAMA, TAKASHI; MIYAJIMA, MASAYASU; NEGISHI, TAKAYUKI; KAWASAKI, TAKAHIKO; TAKAMATSU, HYOTA; KIKUTANI, HITOSHI; KUMANOGOH, ATSUSHI; YUKAWA, KAZUNORI

2015-01-01

The opening of the mouse vaginal cavity to the skin is a postnatal tissue remodeling process that occurs at approximately five weeks of age for the completion of female genital tract maturation at puberty. The tissue remodeling process is primarily composed of a hormonally triggered apoptotic process predominantly occurring in the epithelium of the distal section of the vaginal cavity. However, the detailed mechanism underlying the apoptotic induction remains to be elucidated. In the present study, it was observed that the majority of BALB/c mice lacking the class 4 semaphorin, semaphorin 4D (Sema4D), developed imperforate vagina and hydrometrocolpos resulting in a perpetually unopened vaginal cavity regardless of a normal estrogen level comparable with that in wild-type (WT) mice. Administration of β-estradiol to infant Sema4D-deficient (Sema4D−/−) mice did not induce precocious vaginal opening, which was observed in WT mice subjected to the same β-estradiol administration, excluding the possibility that the closed vaginal phenotype was due to insufficient estrogen secretion at the time of vaginal opening. In order to assess the role of Sema4D in the postnatal vaginal tissue remodeling process, the expression of Sema4D and its receptor, plexin-B1, was examined as well as the level of apoptosis in the vaginal epithelia of five-week-old WT and Sema4D−/− mice. Immunohistochemical analyses confirmed the localization of Sema4D and plexin-B1 in the mouse vaginal epithelia. Terminal deoxynucleotidyl transferase dUTP nick end labeling assay and immunohistochemistry detecting activated caspase-3 revealed significantly fewer apoptotic cells in situ in the vaginal mucosa of five-week-old Sema4D−/− mice compared with WT mice. The addition of recombinant Sema4D to Sema4D−/− vaginal epithelial cells in culture significantly enhanced apoptosis of the vaginal epithelial cells, demonstrating the apoptosis-inducing activity of Sema4D. The experimental reduction of plexin-B1 expression in vaginal epithelial cells demonstrated the integral role of plexin-B1 in Sema4D-induced apoptotic cell death. These results suggest a non-redundant role of Sema4D in the postnatal tissue remodeling process in five-week-old BALB/c mice, which involves the induction of vaginal epithelial cell apoptosis through Sema4D binding to plexin-B1. PMID:25351707

Semaphorin 3A - a marker for disease activity and a potential putative disease-modifying treatment in systemic lupus erythematosus.

PubMed

Vadasz, Z; Toubi, E

2012-10-01

Semaphorin 3A (sema3A) and neuropilin-1 (NP-1) play a regulatory role in immune responses and have a demonstrated effect on the course of collagen-induced arthritis. Sema3A was also found to be involved in other immune-mediated diseases, e.g. psoriasis and allergic rhinitis. In this review we concentrated on the involvement of sema3A and NP-1 in the pathogenesis of systemic lupus erythematosus (SLE) and on the specific effect of sema3A on the auto-reactive properties of B cells in SLE patients. We demonstrated the expression of sema3A in renal biopsies from lupus glomerulonephritis patients. This expression was found to be inversely correlated with proteinuria and kidney function tests. Sema3A serum levels in SLE patients were found to be significantly lower than in RA patients (disease control) and lower yet than in normal individuals. Altered serum sema3A levels were found to be in inverse correlation with SLE disease activity, mainly with renal damage and the presence of anti-cardiolipin antibodies. The expression of both sema3A and NP-1 on B cells from SLE patients was significantly different in comparison with normal healthy individuals. Finally, we demonstrated that when sema3A was co-cultured with CpG-ODN-stimulated memory B cells of SLE patients, their TLR-9 expression was significantly reduced by almost 50% (p = 0.001). These findings, along with the observation of sema3A being reduced in SLE patients in correlation with disease severity and autoimmunity, and memory B cells being beneficially responsive to sema3A, suggest this regulatory molecule may be considered as a potential therapy for SLE. Such focused therapies will help in achieving the maintenance of self-tolerance and alter pro-inflammatory status in lupus.

Anti-SEMA3A Antibody: A Novel Therapeutic Agent to Suppress GBM Tumor Growth.

PubMed

Lee, Jaehyun; Shin, Yong Jae; Lee, Kyoungmin; Cho, Hee Jin; Sa, Jason K; Lee, Sang-Yun; Kim, Seok-Hyung; Lee, Jeongwu; Yoon, Yeup; Nam, Do-Hyun

2017-11-10

Glioblastoma (GBM) is classified as one of the most aggressive and lethal brain tumor. Great strides have been made in understanding the genomic and molecular underpinnings of GBM, which translated into development of new therapeutic approaches to combat such deadly disease. However, there are only few therapeutic agents that can effectively inhibit GBM invasion in a clinical framework. In an effort to address such challenges, we have generated anti-SEMA3A monoclonal antibody as a potential therapeutic antibody against GBM progression. We employed public glioma datasets, Repository of Molecular Brain Neoplasia Data and The Cancer Genome Atlas, to analyze SEMA3A mRNA expression in human GBM specimens. We also evaluated for protein expression level of SEMA3A via tissue microarray (TMA) analysis. Cell migration and proliferation kinetics were assessed in various GBM patient-derived cells (PDCs) and U87-MG cell-line for SEMA3A antibody efficacy. GBM patient-derived xenograft (PDX) models were generated to evaluate tumor inhibitory effect of anti-SEMA3A antibody in vivo. By combining bioinformatics and TMA analysis, we discovered that SEMA3A is highly expressed in human GBM specimens compared to non-neoplastic tissues. We developed three different anti-SEMA3A antibodies, in fully human IgG form, through screening phage-displayed synthetic antibody library using a classical panning method. Neutralization of SEMA3A significantly reduced migration and proliferation capabilities of PDCs and U87-MG cell-line in vitro. In PDX models, treatment with anti-SEMA3A antibody exhibited notable tumor inhibitory effect through down-regulation of cellular proliferative kinetics and tumor-associated macrophages recruitment. In present study, we demonstrated tumor inhibitory effect of SEMA3A antibody in GBM progression and present its potential relevance as a therapeutic agent in a clinical framework.

mTOR Complex Signaling through the SEMA4A-Plexin B2 Axis Is Required for Optimal Activation and Differentiation of CD8+ T Cells.

PubMed

Ito, Daisuke; Nojima, Satoshi; Nishide, Masayuki; Okuno, Tatsusada; Takamatsu, Hyota; Kang, Sujin; Kimura, Tetsuya; Yoshida, Yuji; Morimoto, Keiko; Maeda, Yohei; Hosokawa, Takashi; Toyofuku, Toshihiko; Ohshima, Jun; Kamimura, Daisuke; Yamamoto, Masahiro; Murakami, Masaaki; Morii, Eiichi; Rakugi, Hiromi; Isaka, Yoshitaka; Kumanogoh, Atsushi

2015-08-01

Mammalian target of rapamycin (mTOR) plays crucial roles in activation and differentiation of diverse types of immune cells. Although several lines of evidence have demonstrated the importance of mTOR-mediated signals in CD4(+) T cell responses, the involvement of mTOR in CD8(+) T cell responses is not fully understood. In this study, we show that a class IV semaphorin, SEMA4A, regulates CD8(+) T cell activation and differentiation through activation of mTOR complex (mTORC) 1. SEMA4A(-/-) CD8(+) T cells exhibited impairments in production of IFN-γ and TNF-α and induction of the effector molecules granzyme B, perforin, and FAS-L. Upon infection with OVA-expressing Listeria monocytogenes, pathogen-specific effector CD8(+) T cell responses were significantly impaired in SEMA4A(-/-) mice. Furthermore, SEMA4A(-/-) CD8(+) T cells exhibited reduced mTORC1 activity and elevated mTORC2 activity, suggesting that SEMA4A is required for optimal activation of mTORC1 in CD8(+) T cells. IFN-γ production and mTORC1 activity in SEMA4A(-/-) CD8(+) T cells were restored by administration of recombinant Sema4A protein. In addition, we show that plexin B2 is a functional receptor of SEMA4A in CD8(+) T cells. Collectively, these results not only demonstrate the role of SEMA4A in CD8(+) T cells, but also reveal a novel link between a semaphorin and mTOR signaling. Copyright © 2015 by The American Association of Immunologists, Inc.

Semaphorin 3A in Ankylosing Spondylitis.

PubMed

Liao, Hsien-Tzung; Lin, Yuh-Feng; Chou, Chung-Tei; Tsai, Chang-Youh

2017-07-14

To determine serum semaphorin 3A (Sema 3A) levels in ankylosing spondylitis (AS). Serum Sema 3A was measured in 46 AS patients and 30 healthy controls (HCs). For the patients, we recorded demographic data, disease activity, functional index & global assessment, detected human leukocyte antigen-B27 (HLA-B27), and measured erythrocyte sedimentation rate (ESR) & C-reactive protein (CRP). Sema 3A was higher in AS patients than in HCs (3.98 ± 2.57 vs. 1.34 ± 0.48 ng/ml, p = 0.013). Area under the curve (AUC) of standard receiver operating characteristic (ROC) has suggested that Sema 3A > 2 ng/ml is better to predict the higher Bath Ankylosing Spondylitis Disease Activity Index (BASDAI, > 4) than ESR or CRP. There were good correlations between higher Sema 3A and uveitis, Schöber's test, as well as interstitial lung disease. AS patients undergoing anti-tumor necrosis factor therapies for 3 months exhibited a positive correlation of change in Sema 3A (ΔSema 3A) with disease activity fluctuation [ΔBASDAI, ΔBath Ankylosing Spondylitis Functional Index (BASFI) and ΔBath Ankylosing Spondylitis - Global score (BAS-G)]. Serum Sema 3A level was increased in AS patients and was inversely correlated to Schöber's test. Serum Sema 3A is better as a bio-marker than ESR or CRP to correlate with high disease activity in AS patients, and it is also a good indicator for monitoring disease activity and functional status during anti-TNF treatment. Also, Sema 3A may be taken as a predictor for extra-articular presentations in AS, but this needs further study to elucidate. Copyright © 2017. Published by Elsevier B.V.

Prostate cancer cells induce osteoblastic differentiation via semaphorin 3A.

PubMed

Liu, Fuzhou; Shen, Weiwei; Qiu, Hao; Hu, Xu; Zhang, Chao; Chu, Tongwei

2015-03-01

Prostate cancer metastasis to bone is the second most commonly diagnosed malignant disease among men worldwide. Such metastatic disease is characterized by the presence of osteoblastic bone lesions, and is associated with high rates of mortality. However, the various mechanisms involved in prostate cancer-induced osteoblastic differentiation have not been fully explored. Semaphorin 3A (Sema 3A) is a newly identified regulator of bone metabolism which stimulates differentiation of pre-osteoblastic cells under physiological conditions. We investigated in this study whether prostate cancer cells can mediate osteoblastic activity through Sema 3A. We cultured osteoprogenitor MC3T3-E1 cells in prostate cancer-conditioned medium, and analyzed levels of Sema 3A protein in diverse prostate cancer cell lines to identify cell lines in which Sema 3A production showed a positive correlation with osteo-stimulation. C4-2 cells were stably transfected with Sema 3A short hairpin RNA to further determine whether Sema 3A contributes to the ability of C4-2 cells to induce osteoblastic differentiation. Down-regulation of Sema 3A expression decreased indicators of C4-2 CM-induced osteoblastic differentiation, including alkaline phosphatase production and mineralization. Additionally, silencing or neutralizing Sema 3A in C4-2 cells resulted in diminished β-catenin expression in osteogenitor MC3T3-E1 cells. Our results suggest that prostate cancer-induced osteoblastic differentiation is at least partially mediated by Sema 3A, and may be regulated by the β-catenin signalling pathway. Sema 3A may represent a novel target for treatment of prostate cancer-induced osteoblastic lesions. © 2014 Wiley Periodicals, Inc.

Semaphorin3A, Neuropilin-1, and PlexinA1 are required for lymphatic valve formation.

PubMed

Bouvrée, Karine; Brunet, Isabelle; Del Toro, Raquel; Gordon, Emma; Prahst, Claudia; Cristofaro, Brunella; Mathivet, Thomas; Xu, Yunling; Soueid, Jihane; Fortuna, Vitor; Miura, Nayoki; Aigrot, Marie-Stéphane; Maden, Charlotte H; Ruhrberg, Christiana; Thomas, Jean Léon; Eichmann, Anne

2012-08-03

The lymphatic vasculature plays a major role in fluid homeostasis, absorption of dietary lipids, and immune surveillance. Fluid transport depends on the presence of intraluminal valves within lymphatic collectors. Defective formation of lymphatic valves leads to lymphedema, a progressive and debilitating condition for which curative treatments are currently unavailable. How lymphatic valve formation is regulated remains largely unknown. We investigated if the repulsive axon guidance molecule Semaphorin3A (Sema3A) plays a role in lymphatic valve formation. We show that Sema3A mRNA is expressed in lymphatic vessels and that Sema3A protein binds to lymphatic valves expressing the Neuropilin-1 (Nrp1) and PlexinA1 receptors. Using mouse knockout models, we show that Sema3A is selectively required for lymphatic valve formation, via interaction with Nrp1 and PlexinA1. Sema3a(-/-) mice exhibit defects in lymphatic valve formation, which are not due to abnormal lymphatic patterning or sprouting, and mice carrying a mutation in the Sema3A binding site of Nrp1, or deficient for Plxna1, develop lymphatic valve defects similar to those seen in Sema3a(-/-) mice. Our data demonstrate an essential direct function of Sema3A-Nrp1-PlexinA1 signaling in lymphatic valve formation.

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Material Modeling for Terminal Ballistic Simulation

DTIC Science & Technology

1992-09-01

DYNA-3D-a nonlinear, explicit, three-dimensional finite element code for solid and structural mechanics- user manual. Technical Report UCRL -MA...Rep. UCRL -50108, Rev. 1, Lawrence Livermore Laboratory, 1977. [34] S. P. Marsh. LASL Shock Hugoniot Data. University of California Press, Berkeley, CA...Steinberg. Equation of state and strength properties of selected ma- teriaJs. Tech. Rep. UCRL -MA-106439, Lawrence Livermore National Labo- ratory, 1991. [371

Evaluation of a Standardized Extract from Morus alba against α-Glucosidase Inhibitory Effect and Postprandial Antihyperglycemic in Patients with Impaired Glucose Tolerance: A Randomized Double-Blind Clinical Trial

PubMed Central

Hwang, Seung Hwan; Li, Hong Mei; Wang, Zhiqiang

2016-01-01

To evaluate the antihyperglycemic effect of a standardized extract of the leaves of Morus alba (SEMA), the present study was designed to investigate the α-glucosidase inhibitory effect and acute single oral toxicity as well as evaluate blood glucose reduction in animals and in patients with impaired glucose tolerance in a randomized double-blind clinical trial. SEMA was found to inhibit α-glucosidase at a fourfold higher level than the positive control (acarbose), in a concentration-dependent manner. Moreover, blood glucose concentration was suppressed by SEMA in vivo. Clinical signs and weight changes were observed when conducting an evaluation of the acute toxicity of SEMA through a single-time administration, with clinical observation conducted more than once each day. After administration of the SEMA, observation was for 14 days; all of the animals did not die and did not show any abnormal symptoms. In addition, the inhibitory effects of rice coated with SEMA were evaluated in a group of impaired glucose tolerance patients on postprandial glucose and a group of normal persons, and results showed that SEMA had a clear inhibitory effect on postprandial hyperglycemia in both groups. Overall, SEMA showed excellent potential in the present study as a material for improving postprandial hyperglycemia. PMID:27974904

Semaphorin3A, Neuropilin-1, and PlexinA1 Are Required for Lymphatic Valve Formation

PubMed Central

Bouvrée, Karine; Brunet, Isabelle; del Toro, Raquel; Gordon, Emma; Prahst, Claudia; Cristofaro, Brunella; Mathivet, Thomas; Xu, Yunling; Soueid, Jihane; Fortuna, Vitor; Miura, Nayoki; Aigrot, Marie-Stéphane; Maden, Charlotte H.; Ruhrberg, Christiana; Thomas, Jean Léon; Eichmann, Anne

2013-01-01

Rationale The lymphatic vasculature plays a major role in fluid homeostasis, absorption of dietary lipids, and immune surveillance. Fluid transport depends on the presence of intraluminal valves within lymphatic collectors. Defective formation of lymphatic valves leads to lymphedema, a progressive and debilitating condition for which curative treatments are currently unavailable. How lymphatic valve formation is regulated remains largely unknown. Objective We investigated if the repulsive axon guidance molecule Semaphorin3A (Sema3A) plays a role in lymphatic valve formation. Methods and Results We show that Sema3A mRNA is expressed in lymphatic vessels and that Sema3A protein binds to lymphatic valves expressing the Neuropilin-1 (Nrp1) and PlexinA1 receptors. Using mouse knockout models, we show that Sema3A is selectively required for lymphatic valve formation, via interaction with Nrp1 and PlexinA1. Sema3a−/− mice exhibit defects in lymphatic valve formation, which are not due to abnormal lymphatic patterning or sprouting, and mice carrying a mutation in the Sema3A binding site of Nrp1, or deficient for Plxna1, develop lymphatic valve defects similar to those seen in Sema3a−/− mice. Conclusions Our data demonstrate an essential direct function of Sema3A-Nrp1-PlexinA1 signaling in lymphatic valve formation. PMID:22723296

Anti-proliferative effects of gold nanoparticles functionalized with Semaphorin 3F

NASA Astrophysics Data System (ADS)

Tan, Gamze; Onur, Mehmet Ali

2017-08-01

The new vessel formations play a vital role in growth and spread of cancer. Current anti-angiogenic therapies, predominantly based on vascular endothelial growth factor (VEGF) inhibition, can inhibit vascular development; however, they are usually ineffective against the primary tumor occurrence. The aim of this study was to assess anti-angiogenic effects of gold nanoparticles (AuNPs) functionalized with Semaphorin (Sema) 3F protein. The polyethylene glycol (PEG)-coated AuNPs were covalently functionalized with Sema 3F and labeled with the TAMRA fluorescent dye. The effect of the NPs on human umbilical vein endothelial cells (HUVECs) is probed in the way of internalization and viability assays. AuNP-Sema 3F bioconjugates showed great endothelial cell uptake. AuNP-Sema 3F bioconjugates reduced VEGF165-induced endothelial cell proliferation more effectively than Sema 3F alone, suggesting that the therapeutic effects of Sema 3F can be improved by conjugation to AuNPs. Also, no significant toxicity effect was induced by bioconjugates. This is the first study that reports a covalent binding of full length Sema 3F to NPs. The exogenously administration of Sema 3F, which has both anti-angiogenic and anti-tumoral activity, to tumor vasculature via a carrying platform may not only lead to more effective anti-angiogenic treatment but also may make current approach more applicable in clinical use like drug delivery system. [Figure not available: see fulltext.

A novel podocyte gene, semaphorin 3G, protects glomerular podocyte from lipopolysaccharide-induced inflammation.

PubMed

Ishibashi, Ryoichi; Takemoto, Minoru; Akimoto, Yoshihiro; Ishikawa, Takahiro; He, Peng; Maezawa, Yoshiro; Sakamoto, Kenichi; Tsurutani, Yuya; Ide, Shintaro; Ide, Kana; Kawamura, Harukiyo; Kobayashi, Kazuki; Tokuyama, Hirotake; Tryggvason, Karl; Betsholtz, Christer; Yokote, Koutaro

2016-05-16

Kidney diseases including diabetic nephropathy have become huge medical problems, although its precise mechanisms are still far from understood. In order to increase our knowledge about the patho-physiology of kidney, we have previously identified >300 kidney glomerulus-enriched transcripts through large-scale sequencing and microarray profiling of the mouse glomerular transcriptome. One of the glomerulus-specific transcripts identified was semaphorin 3G (Sema3G) which belongs to the semaphorin family. The aim of this study was to analyze both the in vivo and in vitro functions of Sema3G in the kidney. Sema3G was expressed in glomerular podocytes. Although Sema3G knockout mice did not show obvious glomerular defects, ultrastructural analyses revealed partially aberrant podocyte foot processes structures. When these mice were injected with lipopolysaccharide to induce acute inflammation or streptozotocin to induce diabetes, the lack of Sema3G resulted in increased albuminuria. The lack of Sema3G in podocytes also enhanced the expression of inflammatory cytokines including chemokine ligand 2 and interleukin 6. On the other hand, the presence of Sema3G attenuated their expression through the inhibition of lipopolysaccharide-induced Toll like receptor 4 signaling. Taken together, our results surmise that the Sema3G protein is secreted by podocytes and protects podocytes from inflammatory kidney diseases and diabetic nephropathy.

A novel podocyte gene, semaphorin 3G, protects glomerular podocyte from lipopolysaccharide-induced inflammation

PubMed Central

Ishibashi, Ryoichi; Takemoto, Minoru; Akimoto, Yoshihiro; Ishikawa, Takahiro; He, Peng; Maezawa, Yoshiro; Sakamoto, Kenichi; Tsurutani, Yuya; Ide, Shintaro; Ide, Kana; Kawamura, Harukiyo; Kobayashi, Kazuki; Tokuyama, Hirotake; Tryggvason, Karl; Betsholtz, Christer; Yokote, Koutaro

2016-01-01

Kidney diseases including diabetic nephropathy have become huge medical problems, although its precise mechanisms are still far from understood. In order to increase our knowledge about the patho-physiology of kidney, we have previously identified >300 kidney glomerulus-enriched transcripts through large-scale sequencing and microarray profiling of the mouse glomerular transcriptome. One of the glomerulus-specific transcripts identified was semaphorin 3G (Sema3G) which belongs to the semaphorin family. The aim of this study was to analyze both the in vivo and in vitro functions of Sema3G in the kidney. Sema3G was expressed in glomerular podocytes. Although Sema3G knockout mice did not show obvious glomerular defects, ultrastructural analyses revealed partially aberrant podocyte foot processes structures. When these mice were injected with lipopolysaccharide to induce acute inflammation or streptozotocin to induce diabetes, the lack of Sema3G resulted in increased albuminuria. The lack of Sema3G in podocytes also enhanced the expression of inflammatory cytokines including chemokine ligand 2 and interleukin 6. On the other hand, the presence of Sema3G attenuated their expression through the inhibition of lipopolysaccharide-induced Toll like receptor 4 signaling. Taken together, our results surmise that the Sema3G protein is secreted by podocytes and protects podocytes from inflammatory kidney diseases and diabetic nephropathy. PMID:27180624

A Nonsynonymous Polymorphism in Semaphorin 3A as a Risk Factor for Human Unexplained Cardiac Arrest with Documented Ventricular Fibrillation

PubMed Central

Nakano, Yukiko; Chayama, Kazuaki; Ochi, Hidenori; Toshishige, Masaaki; Hayashida, Yasufumi; Miki, Daiki; Hayes, C. Nelson; Suzuki, Hidekazu; Tokuyama, Takehito; Oda, Noboru; Suenari, Kazuyoshi; Uchimura-Makita, Yuko; Kajihara, Kenta; Sairaku, Akinori; Motoda, Chikaaki; Fujiwara, Mai; Watanabe, Yoshikazu; Yoshida, Yukihiko; Ohkubo, Kimie; Watanabe, Ichiro; Nogami, Akihiko; Hasegawa, Kanae; Watanabe, Hiroshi; Endo, Naoto; Aiba, Takeshi; Shimizu, Wataru; Ohno, Seiko; Horie, Minoru; Arihiro, Koji; Tashiro, Satoshi; Makita, Naomasa; Kihara, Yasuki

2013-01-01

Unexplained cardiac arrest (UCA) with documented ventricular fibrillation (VF) is a major cause of sudden cardiac death. Abnormal sympathetic innervations have been shown to be a trigger of ventricular fibrillation. Further, adequate expression of SEMA3A was reported to be critical for normal patterning of cardiac sympathetic innervation. We investigated the relevance of the semaphorin 3A (SEMA3A) gene located at chromosome 5 in the etiology of UCA. Eighty-three Japanese patients diagnosed with UCA and 2,958 healthy controls from two different geographic regions in Japan were enrolled. A nonsynonymous polymorphism (I334V, rs138694505A>G) in exon 10 of the SEMA3A gene identified through resequencing was significantly associated with UCA (combined P = 0.0004, OR 3.08, 95%CI 1.67–5.7). Overall, 15.7% of UCA patients carried the risk genotype G, whereas only 5.6% did in controls. In patients with SEMA3A I334V, VF predominantly occurred at rest during the night. They showed sinus bradycardia, and their RR intervals on the 12-lead electrocardiography tended to be longer than those in patients without SEMA3A I334V (1031±111 ms versus 932±182 ms, P = 0.039). Immunofluorescence staining of cardiac biopsy specimens revealed that sympathetic nerves, which are absent in the subendocardial layer in normal hearts, extended to the subendocardial layer only in patients with SEMA3A I334V. Functional analyses revealed that the axon-repelling and axon-collapsing activities of mutant SEMA3A I334V genes were significantly weaker than those of wild-type SEMA3A genes. A high incidence of SEMA3A I334V in UCA patients and inappropriate innervation patterning in their hearts implicate involvement of the SEMA3A gene in the pathogenesis of UCA. PMID:23593010

Autocrine class 3 semaphorin system regulates slit diaphragm proteins and podocyte survival.

PubMed

Guan, F; Villegas, G; Teichman, J; Mundel, P; Tufro, A

2006-05-01

Class 3 semaphorins are guidance proteins that play crucial roles during development. Semaphorins 3A (sema 3A) and 3F are expressed by podocytes in vivo throughout ontogeny and their function is unknown. Here we examined the expression of class 3 semaphorins (3A, 3B, 3C, 3D, 3E, and 3F) and their receptors (neuropilins 1 and 2, plexins A1, A2, A3, B2, and D1) in undifferentiated and differentiated mouse podocytes using reverse transcriptase-polymerase chain reaction (RT-PCR). All class 3 semaphorins, neuropilins 1 and 2 are expressed by undifferentiated and differentiated podocytes at similar levels. Differentiated podocytes expressed 2-4-fold higher plexin A1, A2, and A3 mRNA levels than undifferentiated podocytes. To examine semaphorin regulation, we exposed podocytes to recombinant sema 3A. Sema 3A decreased semaphorin 3B, plexin A1, A3, and D1 >/=50% and reduced plexin A2 mRNA to undetectable levels. To identify sema 3A function in podocytes, we examined whether sema 3A regulates slit diaphragm proteins and podocyte survival. Sema 3A induced a dose-response podocin downregulation and decreased its interaction with CD2-associated protein and nephrin, as determined by Western analysis and co-immunoprecipitation. To evaluate sema 3A role in podocyte survival, we quantified podocyte apoptosis using a caspase 3 activity marker. Sema 3A induced a 10-fold increase in podocyte apoptosis and significantly decreased the activity of the Akt survival pathway. Our data indicate that (1) immortalized podocytes in culture have a functional autocrine semaphorin system that is regulated by differentiation and ligand availability; (2) sema 3A signaling regulates the expression and interactions of slit-diaphragm proteins and decreases podocyte survival.

SEMA3A suspended in matrigel improves titanium implant fixation in ovariectomized rats.

PubMed

Li, Yunfeng; He, Dongming; Liu, Biao; Hu, Jing

2017-10-01

The aim of this study was to evaluate the effect of SEMA3A released from matrigel on implant fixation in ovariectomized (OVX) rats. Sixty female rats were subjected to bilateral ovariectomy. Twelve weeks later, rats were randomly divided into three groups according to implants they accepted: (1) Control, implants with distilled water; (2) Matrigel, implants with matrigel coating; (3) Matrigel + SEMA3A, implants with coating of SEMA3A suspended in matrigel. Implants were inserted in metaphysis of proximal tibiae in all animas bilaterally. In vitro release of SEMA3A was tested using enzyme linked immunosorbent assay. In vitro release of SEMA3A was detectable during the first 10 days, and a burst release of was observed during the first 3 days. No significant difference was observed between Control and Matrigel group. The protective effects of SEMA3A in matrigel on peri-implant bone, implant osseointegration and fixation was confirmed. Compared to matrigel alone, SEMA3A suspended in matrigel increased percent bone volume by 88.7% and 83.3% (p < 0.01), bone-to-implant contact ratio by 148.9% (p < 0.01), and 24.8% (p < 0.05), the maximal push-out force by 149.3% and 209.2% (p < 0.01) at 4 and 8 weeks after implant insertion, respectively. Surface modification with SEMA3A suspended in matrigel improved implant osseointegration and fixation in the proximal tibiae of OVX rats. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2060-2065, 2017. © 2016 Wiley Periodicals, Inc.

ADAM metalloproteases promote a developmental switch in responsiveness to the axonal repellant Sema3A.

PubMed

Romi, Erez; Gokhman, Irena; Wong, Eitan; Antonovsky, Niv; Ludwig, Andreas; Sagi, Irit; Saftig, Paul; Tessier-Lavigne, Marc; Yaron, Avraham

2014-06-05

During embryonic development, axons can gain and lose sensitivity to guidance cues, and this flexibility is essential for the correct wiring of the nervous system. Yet, the underlying molecular mechanisms are largely unknown. Here we show that receptor cleavage by ADAM (A Disintegrin And Metalloprotease) metalloproteases promotes murine sensory axons loss of responsiveness to the chemorepellant Sema3A. Genetic ablation of ADAM10 and ADAM17 disrupts the developmental downregulation of Neuropilin-1 (Nrp1), the receptor for Sema3A, in sensory axons. Moreover, this is correlated with gain of repulsive response to Sema3A. Overexpression of Nrp1 in neurons reverses axonal desensitization to Sema3A, but this is hampered in a mutant Nrp1 with high susceptibility to cleavage. Lastly, we detect guidance errors of proprioceptive axons in ADAM knockouts that are consistent with enhanced response to Sema3A. Our results provide the first evidence for involvement of ADAMs in regulating developmental switch in responsiveness to axonal guidance cues.

Dysfunctional SEMA3E signaling underlies gonadotropin-releasing hormone neuron deficiency in Kallmann syndrome.

PubMed

Cariboni, Anna; André, Valentina; Chauvet, Sophie; Cassatella, Daniele; Davidson, Kathryn; Caramello, Alessia; Fantin, Alessandro; Bouloux, Pierre; Mann, Fanny; Ruhrberg, Christiana

2015-06-01

Individuals with an inherited deficiency in gonadotropin-releasing hormone (GnRH) have impaired sexual reproduction. Previous genetic linkage studies and sequencing of plausible gene candidates have identified mutations associated with inherited GnRH deficiency, but the small number of affected families and limited success in validating candidates have impeded genetic diagnoses for most patients. Using a combination of exome sequencing and computational modeling, we have identified a shared point mutation in semaphorin 3E (SEMA3E) in 2 brothers with Kallmann syndrome (KS), which causes inherited GnRH deficiency. Recombinant wild-type SEMA3E protected maturing GnRH neurons from cell death by triggering a plexin D1-dependent (PLXND1-dependent) activation of PI3K-mediated survival signaling. In contrast, recombinant SEMA3E carrying the KS-associated mutation did not protect GnRH neurons from death. In murine models, lack of either SEMA3E or PLXND1 increased apoptosis of GnRH neurons in the developing brain, reducing innervation of the adult median eminence by GnRH-positive neurites. GnRH neuron deficiency in male mice was accompanied by impaired testes growth, a characteristic feature of KS. Together, these results identify SEMA3E as an essential gene for GnRH neuron development, uncover a neurotrophic function for SEMA3E in the developing brain, and elucidate SEMA3E/PLXND1/PI3K signaling as a mechanism that prevents GnRH neuron deficiency.

Driving Extreme Efficiency to Market

NASA Astrophysics Data System (ADS)

Garbesi, Karina

2014-03-01

The rapid development of extremely energy efficient appliances and equipment is essential to curtail catastrophic climate disruption. This will require the on-going development of products that apply all best-practices and that take advantage of the synergies of hybridization and building integration. Beyond that, it requires the development of new disruptive technologies and concepts. To facilitate these goals, in 2011 the Lawrence Berkeley National Laboratory and the U.S. Department of Energy launched the Max Tech and Beyond Design Competition for Ultra-Low-Energy-Use Appliances and Equipment. Now in its third year, the competition supports faculty-lead student design teams at U.S. universities to develop and test new technology prototypes. This talk describes what the competition and the Max Tech Program are doing to drive such rapid technology progress and to facilitate the entry to the market of successful Max Tech prototypes. The talk also initiates a discussion of physicists' unique role in driving that technology progress faster and farther. Emerging Technologies, Building Technologies Office, U.S. Department of Energy.

Managing Objects in a Relational Framework

DTIC Science & Technology

1989-01-01

Database Week, San Jose CA, May.1983, pp.107-113. [Stonebraker 85] Stonebraker,M. and Rowe,L.: "The Design of POSTGRES " Tech.Report UC Berkeley, Nov...latter is equivalent to the definition of an attribute in a POSTGRES relation using the generic Quel facility. Recently, recursive query languages have...utilize rewrite rules. OSQL [Lynl 88] provides a language for associative access. 2. The POSTGRES model [Sto 86] allows Quel and C-procedures as the

Semaphorin 4C Protects against Allergic Inflammation: Requirement of Regulatory CD138+ Plasma Cells.

PubMed

Xue, Di; Kaufman, Gabriel N; Dembele, Marieme; Beland, Marianne; Massoud, Amir H; Mindt, Barbara C; Fiter, Ryan; Fixman, Elizabeth D; Martin, James G; Friedel, Roland H; Divangahi, Maziar; Fritz, Jörg H; Mazer, Bruce D

2017-01-01

The regulatory properties of B cells have been studied in autoimmune diseases; however, their role in allergic diseases is poorly understood. We demonstrate that Semaphorin 4C (Sema4C), an axonal guidance molecule, plays a crucial role in B cell regulatory function. Mice deficient in Sema4C exhibited increased airway inflammation after allergen exposure, with massive eosinophilic lung infiltrates and increased Th2 cytokines. This phenotype was reproduced by mixed bone marrow chimeric mice with Sema4C deficient only in B cells, indicating that B lymphocytes were the key cells affected by the absence of Sema4C expression in allergic inflammation. We determined that Sema4C-deficient CD19 + CD138 + cells exhibited decreased IL-10 and increased IL-4 expression in vivo and in vitro. Adoptive transfer of Sema4c -/- CD19 + CD138 + cells induced marked pulmonary inflammation, eosinophilia, and increased bronchoalveolar lavage fluid IL-4 and IL-5, whereas adoptive transfer of wild-type CD19 + CD138 + IL-10 + cells dramatically decreased allergic airway inflammation in wild-type and Sema4c -/- mice. This study identifies a novel pathway by which Th2-mediated immune responses are regulated. It highlights the importance of plasma cells as regulatory cells in allergic inflammation and suggests that CD138 + B cells contribute to cytokine balance and are important for maintenance of immune homeostasis in allergic airways disease. Furthermore, we demonstrate that Sema4C is critical for optimal regulatory cytokine production in CD138 + B cells. Copyright © 2016 by The American Association of Immunologists, Inc.

Flotillin-mediated endocytic events dictate cell type-specific responses to semaphorin 3A.

PubMed

Carcea, Ioana; Ma'ayan, Avi; Mesias, Roxana; Sepulveda, Bryan; Salton, Stephen R; Benson, Deanna L

2010-11-10

Cortical efferents growing in the same environment diverge early in development. The expression of particular transcription factors dictates the trajectories taken, presumably by regulating responsiveness to guidance cues via cellular mechanisms that are not yet known. Here, we show that cortical neurons that are dissociated and grown in culture maintain their cell type-specific identities defined by the expression of transcription factors. Using this model system, we sought to identify and characterize mechanisms that are recruited to produce cell type-specific responses to Semaphorin 3A (Sema3A), a guidance cue that would be presented similarly to cortical axons in vivo. Axons from presumptive corticofugal neurons lacking the transcription factor Satb2 and expressing Ctip2 or Tbr1 respond far more robustly to Sema3A than those from presumptive callosal neurons expressing Satb2. Both populations of axons express similar levels of Sema3A receptors (neuropilin-1, cell adhesion molecule L1, and plexinA4), but significantly, axons from neurons lacking Satb2 internalize more Sema3A, and they do so via a raft-mediated endocytic pathway. We used an in silico approach to identify the endocytosis effector flotillin-1 as a Sema3A signaling candidate. We tested the contributions of flotillin-1 to Sema3A endocytosis and signaling, and show that raft-mediated Sema3A endocytosis is defined by and depends on the recruitment of flotillin-1, which mediates LIM domain kinase activation and regulates axon responsiveness to Sema3A in presumptive corticofugal axons.

ERM proteins regulate growth cone responses to Sema3A.

PubMed

Mintz, C David; Carcea, Ioana; McNickle, Daniel G; Dickson, Tracey C; Ge, Yongchao; Salton, Stephen R J; Benson, Deanna L

2008-10-01

Axonal growth cones initiate and sustain directed growth in response to cues in their environment. A variety of events such as receptor internalization, kinase activation, and actin rearrangement can be stimulated by guidance cues and are essential for mediating targeted growth cone behavior. Surprisingly little is known about how such disparate actions are coordinated. Our data suggest that ezrin, radixin, and moesin (ERMs), a family of highly homologous, multifunctional proteins may be able to coordinate growth cone responses to the guidance cue Semaphorin 3A (Sema3A). We show that active ERMs concentrate asymmetrically in neocortical growth cones, are rapidly and transiently inactivated by Sema3A, and are required for Sema3A-mediated growth cone collapse and guidance. The FERM domain of active ERMs regulates internalization of the Sema3A receptor, Npn1, and its coreceptor, L1CAM, while the ERM C-terminal domain binds and caps F-actin. Our data support a model in which ERMs can coordinate membrane and actin dynamics in response to Sema3A.

Tax and Semaphorin 4D Released from Lymphocytes Infected with Human Lymphotropic Virus Type 1 and Their Effect on Neurite Growth.

PubMed

Quintremil, Sebastián; Alberti, Carolina; Rivera, Matías; Medina, Fernando; Puente, Javier; Cartier, Luis; Ramírez, Eugenio; Tanaka, Yuetsu; Valenzuela, M Antonieta

2016-01-01

Human lymphotropic virus type 1 (HTLV-1) is a retrovirus causing HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), a neurodegenerative central nervous system (CNS) axonopathy. This virus mainly infects CD4(+) T lymphocytes without evidence of neuronal infection. Viral Tax, secreted from infected lymphocytes infiltrated in the CNS, is proposed to alter intracellular pathways related to axonal cytoskeleton dynamics, producing neurological damage. Previous reports showed a higher proteolytic release of soluble Semaphorin 4D (sSEMA-4D) from CD4(+) T cells infected with HTLV-1. Soluble SEMA-4D binds to its receptor Plexin-B1, activating axonal growth collapse pathways in the CNS. In the current study, an increase was found in both SEMA-4D in CD4(+) T cells and sSEMA-4D released to the culture medium of peripheral blood mononuclear cells (PBMCs) from HAM/TSP patients compared to asymptomatic carriers and healthy donors. After a 16-h culture, infected PBMCs showed significantly higher levels of CRMP-2 phosphorylated at Ser(522). The effect was blocked either with anti-Tax or anti-SEMA-4D antibodies. The interaction of Tax and sSEMA-4D was found in secreted medium of PBMCs in patients, which might be associated with a leading role of Tax with the SEMA-4D-Plexin-B1 signaling pathway. In infected PBMCs, the migratory response after transwell assay showed that sSEMA-4D responding cells were CD4(+)Tax(+) T cells with a high CRMP-2 pSer(522) content. In the present study, the participation of Tax-sSEMA-4D in the reduction in neurite growth in PC12 cells produced by MT2 (HTLV-1-infected cell line) culture medium was observed. These results lead to the participation of plexins in the reported effects of infected lymphocytes on neuronal cells.

Tax and Semaphorin 4D Released from Lymphocytes Infected with Human Lymphotropic Virus Type 1 and Their Effect on Neurite Growth

PubMed Central

Quintremil, Sebastián; Alberti, Carolina; Rivera, Matías; Medina, Fernando; Puente, Javier; Cartier, Luis; Ramírez, Eugenio; Tanaka, Yuetsu

2016-01-01

Abstract Human lymphotropic virus type 1 (HTLV-1) is a retrovirus causing HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), a neurodegenerative central nervous system (CNS) axonopathy. This virus mainly infects CD4+ T lymphocytes without evidence of neuronal infection. Viral Tax, secreted from infected lymphocytes infiltrated in the CNS, is proposed to alter intracellular pathways related to axonal cytoskeleton dynamics, producing neurological damage. Previous reports showed a higher proteolytic release of soluble Semaphorin 4D (sSEMA-4D) from CD4+ T cells infected with HTLV-1. Soluble SEMA-4D binds to its receptor Plexin-B1, activating axonal growth collapse pathways in the CNS. In the current study, an increase was found in both SEMA-4D in CD4+ T cells and sSEMA-4D released to the culture medium of peripheral blood mononuclear cells (PBMCs) from HAM/TSP patients compared to asymptomatic carriers and healthy donors. After a 16-h culture, infected PBMCs showed significantly higher levels of CRMP-2 phosphorylated at Ser522. The effect was blocked either with anti-Tax or anti-SEMA-4D antibodies. The interaction of Tax and sSEMA-4D was found in secreted medium of PBMCs in patients, which might be associated with a leading role of Tax with the SEMA-4D-Plexin-B1 signaling pathway. In infected PBMCs, the migratory response after transwell assay showed that sSEMA-4D responding cells were CD4+Tax+ T cells with a high CRMP-2 pSer522 content. In the present study, the participation of Tax-sSEMA-4D in the reduction in neurite growth in PC12 cells produced by MT2 (HTLV-1-infected cell line) culture medium was observed. These results lead to the participation of plexins in the reported effects of infected lymphocytes on neuronal cells. PMID:26389656

The axon guidance molecule semaphorin 3F is a negative regulator of tumor progression and proliferation in ileal neuroendocrine tumors

PubMed Central

Vercherat, Cécile; Blanc, Martine; Lepinasse, Florian; Gadot, Nicolas; Couderc, Christophe; Poncet, Gilles; Walter, Thomas; Joly, Marie-Odile; Hervieu, Valérie; Scoazec, Jean-Yves; Roche, Colette

2015-01-01

Gastro-intestinal neuroendocrine tumors (GI-NETs) are rare neoplasms, frequently metastatic, raising difficult clinical and therapeutic challenges due to a poor knowledge of their biology. As neuroendocrine cells express both epithelial and neural cell markers, we studied the possible involvement in GI-NETs of axon guidance molecules, which have been shown to decrease tumor cell proliferation and metastatic dissemination in several tumor types. We focused on the role of Semaphorin 3F (SEMA3F) in ileal NETs, one of the most frequent subtypes of GI-NETs. SEMA3F expression was detected in normal neuroendocrine cells but was lost in most of human primary tumors and all their metastases. SEMA3F loss of expression was associated with promoter gene methylation. After increasing endogenous SEMA3F levels through stable transfection, enteroendocrine cell lines STC-1 and GluTag showed a reduced proliferation rate in vitro. In two different xenograft mouse models, SEMA3F-overexpressing cells exhibited a reduced ability to form tumors and a hampered liver dissemination potential in vivo. This resulted, at least in part, from the inhibition of mTOR and MAPK signaling pathways. This study demonstrates an anti-tumoral role of SEMA3F in ileal NETs. We thus suggest that SEMA3F and/or its cellular signaling pathway could represent a target for ileal NET therapy. PMID:26447612

Brain Endothelial Cells Control Fertility through Ovarian-Steroid–Dependent Release of Semaphorin 3A

PubMed Central

Messina, Andrea; Casoni, Filippo; Vanacker, Charlotte; Langlet, Fanny; Hobo, Barbara; Cagnoni, Gabriella; Gallet, Sarah; Hanchate, Naresh Kumar; Mazur, Danièle; Taniguchi, Masahiko; Mazzone, Massimiliano; Verhaagen, Joost; Ciofi, Philippe; Bouret, Sébastien G.; Tamagnone, Luca; Prevot, Vincent

2014-01-01

Neuropilin-1 (Nrp1) guides the development of the nervous and vascular systems, but its role in the mature brain remains to be explored. Here we report that the expression of the 65 kDa isoform of Sema3A, the ligand of Nrp1, by adult vascular endothelial cells, is regulated during the ovarian cycle and promotes axonal sprouting in hypothalamic neurons secreting gonadotropin-releasing hormone (GnRH), the neuropeptide controlling reproduction. Both the inhibition of Sema3A/Nrp1 signaling and the conditional deletion of Nrp1 in GnRH neurons counteract Sema3A-induced axonal sprouting. Furthermore, the localized intracerebral infusion of Nrp1- or Sema3A-neutralizing antibodies in vivo disrupts the ovarian cycle. Finally, the selective neutralization of endothelial-cell Sema3A signaling in adult Sema3a loxP/loxP mice by the intravenous injection of the recombinant TAT-Cre protein alters the amplitude of the preovulatory luteinizing hormone surge, likely by perturbing GnRH release into the hypothalamo-hypophyseal portal system. Our results identify a previously unknown function for 65 kDa Sema3A-Nrp1 signaling in the induction of axonal growth, and raise the possibility that endothelial cells actively participate in synaptic plasticity in specific functional domains of the adult central nervous system, thus controlling key physiological functions such as reproduction. PMID:24618750

Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A.

PubMed

Giacobini, Paolo; Parkash, Jyoti; Campagne, Céline; Messina, Andrea; Casoni, Filippo; Vanacker, Charlotte; Langlet, Fanny; Hobo, Barbara; Cagnoni, Gabriella; Gallet, Sarah; Hanchate, Naresh Kumar; Mazur, Danièle; Taniguchi, Masahiko; Mazzone, Massimiliano; Verhaagen, Joost; Ciofi, Philippe; Bouret, Sébastien G; Tamagnone, Luca; Prevot, Vincent

2014-03-01

Neuropilin-1 (Nrp1) guides the development of the nervous and vascular systems, but its role in the mature brain remains to be explored. Here we report that the expression of the 65 kDa isoform of Sema3A, the ligand of Nrp1, by adult vascular endothelial cells, is regulated during the ovarian cycle and promotes axonal sprouting in hypothalamic neurons secreting gonadotropin-releasing hormone (GnRH), the neuropeptide controlling reproduction. Both the inhibition of Sema3A/Nrp1 signaling and the conditional deletion of Nrp1 in GnRH neurons counteract Sema3A-induced axonal sprouting. Furthermore, the localized intracerebral infusion of Nrp1- or Sema3A-neutralizing antibodies in vivo disrupts the ovarian cycle. Finally, the selective neutralization of endothelial-cell Sema3A signaling in adult Sema3aloxP/loxP mice by the intravenous injection of the recombinant TAT-Cre protein alters the amplitude of the preovulatory luteinizing hormone surge, likely by perturbing GnRH release into the hypothalamo-hypophyseal portal system. Our results identify a previously unknown function for 65 kDa Sema3A-Nrp1 signaling in the induction of axonal growth, and raise the possibility that endothelial cells actively participate in synaptic plasticity in specific functional domains of the adult central nervous system, thus controlling key physiological functions such as reproduction.

Semaphorin4D Drives CD8+ T-Cell Lesional Trafficking in Oral Lichen Planus via CXCL9/CXCL10 Upregulations in Oral Keratinocytes.

PubMed

Ke, Yao; Dang, Erle; Shen, Shengxian; Zhang, Tongmei; Qiao, Hongjiang; Chang, Yuqian; Liu, Qing; Wang, Gang

2017-11-01

Chemokine-mediated CD8 + T-cell recruitment is an essential but not well-established event for the persistence of oral lichen planus (OLP). Semaphorin 4D (Sema4D)/CD100 is implicated in immune dysfunction, chemokine modulation, and cell migration, which are critical aspects for OLP progression, but its implication in OLP pathogenesis has not been determined. In this study, we sought to explicate the effect of Sema4D on human oral keratinocytes and its capacity to drive CD8 + T-cell lesional trafficking via chemokine modulation. We found that upregulations of sSema4D in OLP tissues and blood were positively correlated with disease severity and activity. In vitro observation revealed that Sema4D induced C-X-C motif chemokine ligand 9/C-X-C motif chemokine ligand 10 production by binding to plexin-B1 via protein kinase B-NF-κB cascade in human oral keratinocytes, which elicited OLP CD8 + T-cell migration. We also confirmed using clinical samples that elevated C-X-C motif chemokine ligand 9/C-X-C motif chemokine ligand 10 levels were positively correlated with sSema4D levels in OLP lesions and serum. Notably, we determined matrix metalloproteinase-9 as a new proteolytic enzyme for the cleavage of sSema4D from the T-cell surface, which may contribute to the high levels of sSema4D in OLP lesions and serum. Our findings conclusively revealed an amplification feedback loop involving T cells, chemokines, and Sema4D-dependent signal that promotes OLP progression. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

Semaphorin3A: A Potential Therapeutic Tool for Lupus Nephritis.

PubMed

Bejar, Jacob; Kessler, Ofra; Sabag, Adi D; Sabo, Edmond; Itzhak, Ofer Ben; Neufeld, Gera; Vadasz, Zahava

2018-01-01

The immune regulatory properties of semaphorin3A (sema3A) (both innate and adaptive) are well established in many in vitro studies. The injection of sema3A into a mice model of rheumatoid arthritis was proven to be highly beneficial, both in attenuating clinical symptoms and in decreasing inflammatory mechanisms. This study was designed in order to assess the possible therapeutic benefits of sema3A following its injection into female NZB/W mice. Forty-eight NZB/W mice were recruited for this study. Thirty mice were treated as a "prevention group" and 18 were used as a "treatment group." Eight-week-old mice were acclimated and then divided into the two abovementioned groups. The injection of sema3A into young mice (at week 12) before the onset of disease (the prevention group) delayed the appearance of proteinuria. Here, the median time to severe proteinuria was 110 days, 95% CI: 88-131. However, in mice in which the empty vector was injected, the median time to severe proteinuria was 63 days, 95% CI: 0-139. sema3A treatment, significantly reduced renal damage, namely, it prevented the deposition of immune complexes in the glomeruli. When sema3A was injected at the onset of proteinuria (the treatment group), aiming to treat rather than to prevent disease in these mice, survival was increased and the deterioration of proteinuria was delayed. Semaphorin3A is highly beneficial in reducing lupus nephritis in NZB/W mice. It delays the appearance and deterioration of proteinuria, and increases the survival rates in these mice. The regulatory mechanisms of sema3A involve both innate and adaptive immune responses. Further studies will establish the idea of applying sema3A in the treatment of lupus nephritis.

View Point: Semaphorin-3E: An Emerging Modulator of Natural Killer Cell Functions?

PubMed Central

Alamri, Abdulaziz; Soussi Gounni, Abdelilah; Kung, Sam K. P.

2017-01-01

Semaphorin-3E (Sema-3E) is a member of a large family of proteins originally identified as axon guidance cues in neural development. It is expressed in different cell types, such as immune cells, cancer cells, neural cells, and epithelial cells. Subsequently, dys-regulation of Sema-3E expression has been reported in various biological processes that range from cancers to autoimmune and allergic diseases. Recent work in our laboratories revealed a critical immunoregulatory role of Sema-3E in experimental allergic asthma. We further speculate possible immune modulatory function(s) of Sema-3E on natural killer (NK) cells. PMID:29113093

Involvement of Semaphorin (Sema4D) in T-Dependent Activation of B Cells.

PubMed

Kuklina, Е М; Nekrasova, I V; Valieva, Yu V

2017-08-01

The involvement of endogenous semaphorin (Sema4D) into the key stage of T-dependent differentiation of B cells, formation of plasmoblasts, was demonstrated in vitro in T/B cell co-culture under conditions of polyclonal activation of T cells. The effect of semaphorin was not associated with activation of high-affinity Sema4D receptor plexin B1, but involves lowaffinity receptor CD72. These data indicate that Sema4D-dependent signal regulates not only the initial stage of B-cell activation, proliferative response to the antigen, but also further differentiation of B cells into plasma cells.

Structural Basis of Semaphorin-Plexin Recognition and Viral Mimicry from Sema7A and A39R Complexes with PlexinC1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Heli; Juo, Z. Sean; Shim, Ann Hye-Ryong

Repulsive signaling by Semaphorins and Plexins is crucial for the development and homeostasis of the nervous, immune, and cardiovascular systems. Sema7A acts as both an immune and a neural Semaphorin through PlexinC1, and A39R is a Sema7A mimic secreted by smallpox virus. We report the structures of Sema7A and A39R complexed with the Semaphorin-binding module of PlexinC1. Both structures show two PlexinC1 molecules symmetrically bridged by Semaphorin dimers, in which the Semaphorin and PlexinC1 {beta} propellers interact in an edge-on, orthogonal orientation. Both binding interfaces are dominated by the insertion of the Semaphorin's 4c-4d loop into a deep groove inmore » blade 3 of the PlexinC1 propeller. A39R appears to achieve Sema7A mimicry by preserving key Plexin-binding determinants seen in the mammalian Sema7A complex that have evolved to achieve higher affinity binding to the host-derived PlexinC1. The complex structures support a conserved Semaphorin-Plexin recognition mode and suggest that Plexins are activated by dimerization.« less

Comparison of Shear Shredder with Hammermill for Size Reduction of Navy Solid Waste

DTIC Science & Technology

1986-03-01

IVY CASE) IS. $1,249,650 $1,002,420 11" MAJOR EQUIPMENT ,EPL-..EMENT COST $ $737,660 $499,100 144 115 KAXINL’ PRACTICAL PROJECT LIfE YEARS 20 20 116...DC DEFFUELSUPPCEN DFSC-OWE, Alexandria VA DLSIE Army Logistics Mgt Center, Fort Lee . VA DOE Whid’Ocean Tech Div. Tobacco. ’AD ENVIRONMENTAL PROTECHION...UNIVERSITY W.B. Ledbetter . College Station. TX UNIVERSITY OF CALIFORNIA Energy Engineer, Davis CA; Prof E.A. Pearson. Berkeley, CA; CE Dept (Mitchell

Semaphorin 3A is a retrograde cell death signal in developing sympathetic neurons

PubMed Central

Wehner, Amanda B.; Abdesselem, Houari; Dickendesher, Travis L.; Imai, Fumiyasu; Yoshida, Yutaka; Giger, Roman J.; Pierchala, Brian A.

2016-01-01

ABSTRACT During development of the peripheral nervous system, excess neurons are generated, most of which will be lost by programmed cell death due to a limited supply of neurotrophic factors from their targets. Other environmental factors, such as ‘competition factors' produced by neurons themselves, and axon guidance molecules have also been implicated in developmental cell death. Semaphorin 3A (Sema3A), in addition to its function as a chemorepulsive guidance cue, can also induce death of sensory neurons in vitro. The extent to which Sema3A regulates developmental cell death in vivo, however, is debated. We show that in compartmentalized cultures of rat sympathetic neurons, a Sema3A-initiated apoptosis signal is retrogradely transported from axon terminals to cell bodies to induce cell death. Sema3A-mediated apoptosis utilizes the extrinsic pathway and requires both neuropilin 1 and plexin A3. Sema3A is not retrogradely transported in older, survival factor-independent sympathetic neurons, and is much less effective at inducing apoptosis in these neurons. Importantly, deletion of either neuropilin 1 or plexin A3 significantly reduces developmental cell death in the superior cervical ganglia. Taken together, a Sema3A-initiated apoptotic signaling complex regulates the apoptosis of sympathetic neurons during the period of naturally occurring cell death. PMID:27143756

Insufficient OPC migration into demyelinated lesions is a cause of poor remyelination in MS and mouse models.

PubMed

Boyd, Amanda; Zhang, Hui; Williams, Anna

2013-06-01

Failure of remyelination of multiple sclerosis (MS) lesions contributes to neurodegeneration that correlates with chronic disability in patients. Currently, there are no available treatments to reduce neurodegeneration, but one therapeutic approach to fill this unmet need is to promote remyelination. As many demyelinated MS lesions contain plentiful oligodendrocyte precursor cells (OPCs), but no mature myelinating oligodendrocytes, research has previously concentrated on promoting OPC maturation. However, some MS lesions contain few OPCs, and therefore, remyelination failure may also be secondary to OPC recruitment failure. Here, in a series of MS samples, we determined how many lesions contained few OPCs, and correlated this to pathological subtype and expression of the chemotactic molecules Semaphorin (Sema) 3A and 3F. 37 % of MS lesions contained low numbers of OPCs, and these were mostly chronic active lesions, in which cells expressed Sema3A (chemorepellent). To test the hypothesis that differential Sema3 expression in demyelinated lesions alters OPC recruitment and the efficiency of subsequent remyelination, we used a focal myelinotoxic mouse model of demyelination. Adding recombinant (r)Sema3A (chemorepellent) to demyelinated lesions reduced OPC recruitment and remyelination, whereas the addition of rSema3F (chemoattractant), or use of transgenic mice with reduced Sema3A expression increased OPC recruitment and remyelination. We conclude that some MS lesions fail to remyelinate secondary to reduced OPC recruitment, and that chemotactic molecules are involved in the mechanism, providing a new group of drug targets to improve remyelination, with a specific target in the Sema3A receptor neuropilin-1.

Frequent deletion of 3p21.1 region carrying semaphorin 3G and aberrant expression of the genes participating in semaphorin signaling in the epithelioid type of malignant mesothelioma cells.

PubMed

Yoshikawa, Yoshie; Sato, Ayuko; Tsujimura, Tohru; Morinaga, Tomonori; Fukuoka, Kazuya; Yamada, Shusai; Murakami, Aki; Kondo, Nobuyuki; Matsumoto, Seiji; Okumura, Yoshitomo; Tanaka, Fumihiro; Hasegawa, Seiki; Hashimoto-Tamaoki, Tomoko; Nakano, Takashi

2011-12-01

Array-based comparative genomic hybridization analysis was performed on 21 malignant mesothelioma (MM) samples (16 primary cell cultures and 5 cell lines) and two reactive mesothelial hyperplasia (RM) primary cell cultures. The RM samples did not have any genomic losses or gains. In MM samples, deletions in 1p, 3p21, 4q, 9p21, 16p13 and 22q were detected frequently. We focused on 3p21 because this deletion was specific to the epithelioid type. Especially, a deletion in 3p21.1 region carrying seven genes including SEMA3G was found in 52% of MM samples (11 of 14 epithelioid samples). The allele loss of 3p21.1 might be a good marker for the epithelioid MM. A homozygous deletion in this region was detected in two MM primary cell cultures. A heterozygous deletion detected in nine samples contained the 3p21.1 region and 3p21.31 one carrying the candidate tumor suppressor genes such as semaphorin 3F (SEMA3F), SEMA3B and Ras association (RalGDS/AF-6) domain family member 1 (RASSF1A). SEMA3B, 3F and 3G are class 3 semaphorins and inhibit growth by competing with vascular endothelial growth factor (VEGF) through binding to neuropilin. All MM samples downregulated the expression of more than one gene for SEMA3B, 3F and 3G when compared with Met5a, a normal pleura-derived cell line. Moreover, in 12 of 14 epithelioid MM samples the expression level of SEMA3A was lower than that in Met5a and the two RM samples. An augmented expression of VEGFA was detected in half of the MM samples. The expression ratio of VEGFA/SEMA3A was significantly higher in the epithelioid MMs than in Met5a, RMs and the non-epithelioid MMs. Our data suggest that the downregulated expression of SEMA3A and several SEMA3s results in a loss of inhibitory activities in tumor angiogenesis and tumor growth of VEGFA; therefore, it may play an important role on the pathogenesis of the epithelioid type of MM.

The Semaphorin 4D- Plexin-B1- RhoA signaling axis recruits pericytes and regulates vascular permeability through endothelial production of PDGF-B and ANGPTL4

PubMed Central

Zhou, Hua; Yang, Ying-Hua; Basile, John R.

2013-01-01

Semaphorin 4D (SEMA4D) is a member of a family of transmembrane and secreted proteins that have been shown to act through its receptor Plexin-B1 to regulate axon growth cone guidance, lymphocyte activation, and bone density. SEMA4D is also overexpressed by some malignancies and plays a role in tumor-induced angiogenesis similar to vascular endothelial growth factor (VEGF), a protein that has been targeted as part of some cancer therapies. In an attempt to examine the different effects on tumor growth and vascularity for these two pro-angiogenic factors, we previously noted that while inhibition of both VEGF and SEMA4D restricted tumor vascularity and size, vessels forming under conditions of VEGF blockade retained their association with pericytes while those arising in a background of SEMA4D/ Plexin-B1 deficiency did not, an intriguing finding considering that alteration in pericyte association with endothelial cells is an emerging aspect of anti-angiogenic intervention in the treatment of cancer. Here we show through array analysis, immunoblots, migration and co-culture assays and VE-cadherin immunohistochemistry that SEMA4D production by head and neck carcinoma tumor cells induces expression of platelet-derived growth factor-B (PDGF-B) and angiopoietin-like protein 4 (ANGPTL4) from endothelial cells in a Plexin-B1/ Rho-dependent manner, thereby influencing proliferation and differentiation of pericytes and vascular permeability, whereas VEGF lacks these effects. These results partly explain the differences observed between SEMA4D and VEGF in pathological angiogenesis and suggest that targeting SEMA4D function along with VEGF could represent a novel anti-angiogenic therapeutic strategy for the treatment of solid tumors. PMID:24114199

Structural basis of semaphorin–plexin signalling

PubMed Central

Janssen, Bert J. C.; Robinson, Ross A.; Pérez-Brangulí, Francesc; Bell, Christian H.; Mitchell, Kevin J.; Siebold, Christian; Jones, E. Yvonne

2013-01-01

Cell-cell signalling of semaphorin ligands through interaction with plexin receptors is important for the homeostasis and morphogenesis of many tissues and is widely studied for its role in neural connectivity, cancer, cell migration and immune responses1. SEMA4D and Sema6A exemplify two diverse vertebrate, membrane-spanning semaphorin classes (4 and 6) that are capable of direct signalling through members of the two largest plexin classes, B and A, respectively2,3. In the absence of any structural information on the plexin ectodomain or its interaction with semaphorins the extracellular specificity and mechanism controlling plexin signalling has remained unresolved. Here we present crystal structures of cognate complexes of the semaphorin-binding regions of plexins B1 and A2 with semaphorin ectodomains (human PLXNB11–2–SEMA4Decto and murine PlxnA21–4–Sema6Aecto), plus unliganded structures of PlxnA21–4 and Sema6Aecto. These structures, together with biophysical and cellular assays of wild-type and mutant proteins, reveal that semaphorin dimers independently bind two plexin molecules and that signalling is critically dependent on the avidity of the resulting bivalent 2:2 complex (monomeric semaphorin binds plexin but fails to trigger signalling). In combination, our data favour a cell-cell signalling mechanism involving semaphorin-stabilized plexin dimerization, possibly followed by clustering, which is consistent with previous functional data. Furthermore, the shared generic architecture of the complexes, formed through conserved contacts of the amino-terminal seven-bladed β-propeller (sema) domains of both semaphorin and plexin, suggests that a common mode of interaction triggers all semaphorin–plexin based signalling, while distinct insertions within or between blades of the sema domains determine binding specificity. PMID:20877282

AB071. Semaphorin 3D impact in Indonesian Hirschsprung patients

PubMed Central

Iskandar, Kristy; Sunardi, Mukhamad; Gunadi

2017-01-01

Background Hirschsprung disease (HSCR) is a heterogeneous genetic disorder characterized by absence of ganglion cells along the intestines, results in functional bowel obstruction in children. Recently, Semaphorin 3D (SEMA3D) gene has been implicated in the pathogenesis of intestinal ganglionosis. We aimed to conduct a mutation analysis of SEMA3D gene in HSCR patients in Indonesia, a genetically distinct group within Asia. Methods We ascertained 40 patients with HSCR of whom 27 and 13 were males and females, respectively. Subsequently, we performed direct sequencing to clarify the contribution of SEMA3D gene to HSCR development. Results All patients were sporadic HSCR with degree of aganglionosis as follows: short-segment in 39/40 (98%) patients and long-segment in 1/40 (2%) patients. Transanal endorectal pull-through (TEPT) has been the most common definitive surgery (54%), followed by Duhamel (21%), and Soave (14%). Mutation analysis of SEMA3D gene showed no rare variant, but one common variant in exon 17, rs7800072. The risk allele frequency at rs7800072 (C) among HSCR patients were 0.52. Conclusions This result implies that the SEMA3D gene may not have an effect in the molecular pathogenesis of HSCR, particularly in Indonesia. This study is the first report of SEMA3D gene in Asian ancestry. Further study with multicenter and a larger number of samples is necessary to clarify the results.

Semaphorin 6A knockout mice display abnormalities across ethologically-based topographies of exploration and in motor learning.

PubMed

Håkansson, Kerstin; Runker, Annette E; O'Sullivan, Gerard J; Mitchell, Kevin J; Waddington, John L; O'Tuathaigh, Colm M P

2017-02-22

Semaphorins are secreted or membrane-bound proteins implicated in neurodevelopmental processes of axon guidance and cell migration. Exploratory behaviour and motor learning was examined ethologically in Semaphorin 6A (Sema6A) mutant mice. The ethogram of initial exploration in Sema6A knockout mice was characterised by increased rearing to wall with decreased sifting; over subsequent habituation, locomotion, sniffing and rearing to wall were increased, with reduced habituation of rearing seated. Rotarod analysis indicated delayed motor learning in Sema6A heterozygous mutants. Disruption to the axonal guidance and cell migration processes regulated by Sema6A is associated with topographically specific disruption to fundamental aspects of behaviour, namely the ethogram of initial exploration and subsequent habituation to the environment, and motor learning. Copyright © 2017 Elsevier B.V. All rights reserved.

The association between semaphorin 3A levels and gluten-free diet in patients with celiac disease.

PubMed

Kessel, Aharon; Lin, Chen; Vadasz, Zahava; Peri, Regina; Eiza, Nasren; Berkowitz, Drora

2017-11-01

Celiac disease (CD) is an inflammatory disease affecting the small intestine. We aim to assess serum level and expression of semaphorin 3A (Sema3A) on T regulatory (Treg) cells in CD patients. Twenty-six newly diagnosed celiac patients, 13 celiac patients on a gluten-free diet and 16 healthy controls included in the study. Sema3A protein level in the serum of celiac patients was significantly higher compared to healthy group (7.17±1.8ng/ml vs. 5.67±1.5ng/ml, p=0.012). Sema3A expression on Treg cells was statistically lower in celiac patients compared to healthy subjects (p=0.009) and significantly lower in celiac patients compared to celiac patients on gluten free diet (p=0.04). Negative correlation was found between Sema3A on Teg cells and the level of IgA anti-tTG antibodies (r=-0.346, p<0.01) and anti-DGP (r=-0.448, p<0.01). This study suggests involvement of the Sema3A in the pathogenesis of CD. Copyright © 2017 Elsevier Inc. All rights reserved.

Hybrid Nanotechnologies for Detection and Synergistic Therapies for Breast Cancer

DTIC Science & Technology

2012-10-01

5%-TCA TCG ATG GAG GTG CAG CTG GTG GAG-3%) and FdSeq1 and ligated into pCR2.1 TOPO. The ClaI/NotI-digested frag- ment was ligated into the ClaI/NotI...binding of each scFv clone (bold line) and the backgrounds of phycoerythrin-conjugated secondary antibodies ( gray ). FIGURE 3. Targeting of fluorescently...of the neuropilin family, is a high affinity receptor for the semaphorins Sema E and Sema IV but not Sema III. Neuron 19: 547–559. Dallas NA, Gray MJ

Semaphorin-1a prevents Drosophila olfactory projection neuron dendrites from mis-targeting into select antennal lobe regions.

PubMed

Shen, Hung-Chang; Chu, Sao-Yu; Hsu, Tsai-Chi; Wang, Chun-Han; Lin, I-Ya; Yu, Hung-Hsiang

2017-04-01

Elucidating how appropriate neurite patterns are generated in neurons of the olfactory system is crucial for comprehending the construction of the olfactory map. In the Drosophila olfactory system, projection neurons (PNs), primarily derived from four neural stem cells (called neuroblasts), populate their cell bodies surrounding to and distribute their dendrites in distinct but overlapping patterns within the primary olfactory center of the brain, the antennal lobe (AL). However, it remains unclear whether the same molecular mechanisms are employed to generate the appropriate dendritic patterns in discrete AL glomeruli among PNs produced from different neuroblasts. Here, by examining a previously explored transmembrane protein Semaphorin-1a (Sema-1a) which was proposed to globally control initial PN dendritic targeting along the dorsolateral-to-ventromedial axis of the AL, we discover a new role for Sema-1a in preventing dendrites of both uni-glomerular and poly-glomerular PNs from aberrant invasion into select AL regions and, intriguingly, this Sema-1a-deficient dendritic mis-targeting phenotype seems to associate with the origins of PNs from which they are derived. Further, ectopic expression of Sema-1a resulted in PN dendritic mis-projection from a select AL region into adjacent glomeruli, strengthening the idea that Sema-1a plays an essential role in preventing abnormal dendritic accumulation in select AL regions. Taken together, these results demonstrate that Sema-1a repulsion keeps dendrites of different types of PNs away from each other, enabling the same types of PN dendrites to be sorted into destined AL glomeruli and permitting for functional assembly of olfactory circuitry.

Neuropilin 2 Signaling Is Involved in Cell Positioning of Adult-born Neurons through Glycogen Synthase Kinase-3β (GSK3β).

PubMed

Ng, Teclise; Hor, Catherine H H; Chew, Benjamin; Zhao, Jing; Zhong, Zhong; Ryu, Jae Ryun; Goh, Eyleen L K

2016-11-25

Proper positioning of neurons is fundamental for brain functions. However, little is known on how adult-born neurons generated in the hilar side of hippocampal dentate gyrus migrate into the granular cell layer. Because class 3 Semaphorins (Sema3) are involved in dendritic growth of these newborn neurons, we examined whether they are essential for cell positioning. We disrupted Sema3 signaling by silencing neuropilin 1 (NRP1) or 2 (NRP2), the main receptors for Sema3A and Sema3F, in neural progenitors of adult mouse dentate gyrus. Silencing of NRP2, but not NRP1, affected cell positioning of adult newborn neurons. Glycogen synthase kinase-3β (GSK3β) knockdown phenocopied this NRP2 silencing-mediated cell positioning defect, but did not affect dendritic growth. Furthermore, GSK3β is activated upon stimulation with Sema3F, and GSK3β overexpression rescued the cell positioning phenotypes seen in NRP2-deficient neurons. These results point to a new role for NRP2 in the positioning of neurons during adult hippocampal neurogenesis, acting via the GSK3β signaling pathway. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Semaphorin 3 C drives epithelial-to-mesenchymal transition, invasiveness, and stem-like characteristics in prostate cells.

PubMed

Tam, Kevin J; Hui, Daniel H F; Lee, Wilson W; Dong, Mingshu; Tombe, Tabitha; Jiao, Ivy Z F; Khosravi, Shahram; Takeuchi, Ario; Peacock, James W; Ivanova, Larissa; Moskalev, Igor; Gleave, Martin E; Buttyan, Ralph; Cox, Michael E; Ong, Christopher J

2017-09-13

Prostate cancer (PCa) is among the most commonly-occurring cancers worldwide and a leader in cancer-related deaths. Local non-invasive PCa is highly treatable but limited treatment options exist for those with locally-advanced and metastatic forms of the disease underscoring the need to identify mechanisms mediating PCa progression. The semaphorins are a large grouping of membrane-associated or secreted signalling proteins whose normal roles reside in embryogenesis and neuronal development. In this context, semaphorins help establish chemotactic gradients and direct cell movement. Various semaphorin family members have been found to be up- and down-regulated in a number of cancers. One family member, Semaphorin 3 C (SEMA3C), has been implicated in prostate, breast, ovarian, gastric, lung, and pancreatic cancer as well as glioblastoma. Given SEMA3C's roles in development and its augmented expression in PCa, we hypothesized that SEMA3C promotes epithelial-to-mesenchymal transition (EMT) and stem-like phenotypes in prostate cells. In the present study we show that ectopic expression of SEMA3C in RWPE-1 promotes the upregulation of EMT and stem markers, heightened sphere-formation, and cell plasticity. In addition, we show that SEMA3C promotes migration and invasion in vitro and cell dissemination in vivo.

Semaphorin 3F Is a Bifunctional Guidance Cue for Dopaminergic Axons and Controls Their Fasciculation, Channeling, Rostral Growth, and Intracortical Targeting

PubMed Central

Kolk, Sharon M.; Gunput, Rou-Afza F.; Tran, Tracy S.; van den Heuvel, Dianne M. A.; Prasad, Asheeta A.; Hellemons, Anita J. C. G. M.; Adolfs, Youri; Ginty, David D.; Kolodkin, Alex L.; Burbach, J. Peter H.; Smidt, Marten P.; Pasterkamp, R. Jeroen

2010-01-01

Dopaminergic neurons in the mesodiencephalon (mdDA neurons) make precise synaptic connections with targets in the forebrain via the mesostriatal, mesolimbic, and mesoprefrontal pathways. Because of the functional importance of these remarkably complex ascending axon pathways and their implication in human disease, the mechanisms underlying the development of these connections are of considerable interest. Despite extensive in vitro studies, the molecular determinants that ensure the perfect formation of these pathways in vivo remain mostly unknown. Here, we determine the embryonic origin and ontogeny of the mouse mesoprefrontal pathway and use these data to reveal an unexpected requirement for semaphorin 3F (Sema3F) and its receptor neuropilin-2 (Npn-2) during mdDA pathway development using tissue culture approaches and analysis of sema3F−/−, npn-2−/−, and npn-2−/−;TH-Cre mice. We show that Sema3F is a bifunctional guidance cue for mdDA axons, some of which have the remarkable ability to regulate their responsiveness to Sema3F as they develop. During early developmental stages, Sema3F chemorepulsion controls previously uncharacterized aspects of mdDA pathway development through both Npn-2-dependent (axon fasciculation and channeling) and Npn-2-independent (rostral growth) mechanisms. Later on, chemoattraction mediated by Sema3F and Npn-2 is required to orient mdDA axon projections in the cortical plate of the medial prefrontal cortex. This latter finding demonstrates that regulation of axon orientation in the target field occurs by chemoattractive mechanisms, and this is likely to also apply to other neural systems. In all, this study provides a framework for additional dissection of the molecular basis of mdDA pathway development and disease. PMID:19812329

Semaphorin-1a prevents Drosophila olfactory projection neuron dendrites from mis-targeting into select antennal lobe regions

PubMed Central

Chu, Sao-Yu; Wang, Chun-Han; Lin, I-Ya

2017-01-01

Elucidating how appropriate neurite patterns are generated in neurons of the olfactory system is crucial for comprehending the construction of the olfactory map. In the Drosophila olfactory system, projection neurons (PNs), primarily derived from four neural stem cells (called neuroblasts), populate their cell bodies surrounding to and distribute their dendrites in distinct but overlapping patterns within the primary olfactory center of the brain, the antennal lobe (AL). However, it remains unclear whether the same molecular mechanisms are employed to generate the appropriate dendritic patterns in discrete AL glomeruli among PNs produced from different neuroblasts. Here, by examining a previously explored transmembrane protein Semaphorin-1a (Sema-1a) which was proposed to globally control initial PN dendritic targeting along the dorsolateral-to-ventromedial axis of the AL, we discover a new role for Sema-1a in preventing dendrites of both uni-glomerular and poly-glomerular PNs from aberrant invasion into select AL regions and, intriguingly, this Sema-1a-deficient dendritic mis-targeting phenotype seems to associate with the origins of PNs from which they are derived. Further, ectopic expression of Sema-1a resulted in PN dendritic mis-projection from a select AL region into adjacent glomeruli, strengthening the idea that Sema-1a plays an essential role in preventing abnormal dendritic accumulation in select AL regions. Taken together, these results demonstrate that Sema-1a repulsion keeps dendrites of different types of PNs away from each other, enabling the same types of PN dendrites to be sorted into destined AL glomeruli and permitting for functional assembly of olfactory circuitry. PMID:28448523

The Role of Semaphorin 3B (SEMA3B) in the Pathogenesis of Breast Cancer

DTIC Science & Technology

2006-04-01

apoptotic and anti-proliferative effect on cancer lines it is in part by the inhibition of Akt pathway. In conclusion, we hypothesize that VEGF165...autocrine activity and by inhibiting the Akt pathway. 15. SUBJECT TERMS tumor suppressor gene, breast cancer and apoptosis 16. SECURITY...TGFβ TGFR2 Smad4 M D A M B A 54 9 H 12 99 H el a H 46 0 M C F7 ZR -7 5 H 15 7 2 31 GAPDH TGFR1 B. C 2H 24H 48H 72H SEMA3B SEMA3B

Neuropilin asymmetry mediates a left-right difference in habenular connectivity.

PubMed

Kuan, Yung-Shu; Yu, Hung-Hsiang; Moens, Cecilia B; Halpern, Marnie E

2007-03-01

The medial habenular nuclei of the zebrafish diencephalon, which lie bilateral to the pineal complex, exhibit left-right differences in their neuroanatomy, gene expression profiles and axonal projections to the unpaired midbrain target--the interpeduncular nucleus (IPN). Efferents from the left habenula terminate along the entire dorsoventral extent of the IPN, whereas axons from the right habenula project only to the ventral IPN. How this left-right difference in connectivity is established and the factors involved in differential target recognition are unknown. Prior to IPN innervation, we find that only the left habenula expresses the zebrafish homologue of Neuropilin1a (Nrp1a), a receptor for class III Semaphorins (Sema3s). Directional asymmetry of nrp1a expression relies on Nodal signaling and the presence of the left-sided parapineal organ. Loss of Nrp1a, through parapineal ablation or depletion by antisense morpholinos, prevents left habenular neurons from projecting to the dorsal IPN. Selective depletion of Sema3D, but not of other Sema family members, similarly disrupts innervation of the dorsal IPN. Conversely, Sema3D overexpression results in left habenular projections that extend to the dorsal IPN, as well as beyond the target. The results indicate that Sema3D acts in concert with Nrp1a to guide neurons on the left side of the brain to innervate the target nucleus differently than those on the right side.

Sema4D, the ligand for Plexin B1, suppresses c-Met activation and migration and promotes melanocyte survival and growth.

PubMed

Soong, Joanne; Chen, Yulin; Shustef, Elina M; Scott, Glynis A

2012-04-01

Semaphorins are secreted and membrane-bound proteins involved in neural pathfinding, organogenesis, and tumor progression, through Plexin and neuropilin receptors. We recently reported that Plexin B1, the Semaphorin 4D (Sema4D) receptor, is a tumor-suppressor protein for melanoma, which functions, in part, through inhibition of the oncogenic c-Met tyrosine kinase receptor. In this report, we show that Sema4D is a protective paracrine factor for normal human melanocyte survival in response to UV irradiation, and that it stimulates proliferation and regulates the activity of the c-Met receptor. c-Met receptor signaling stimulates melanocyte migration, partly through downregulation of the cell adhesion molecule E-cadherin. Sema4D suppressed activation of c-Met in response to its ligand, hepatocyte growth factor (HGF), and partially blocked the suppressive effects of HGF on E-cadherin expression in melanocytes and HGF-dependent migration. These data demonstrate a role for Plexin B1 in maintenance of melanocyte survival and proliferation in the skin, and suggest that Sema4D and Plexin B1 act cooperatively with HGF and c-Met to regulate c-Met-dependent effects in human melanocytes. Because our data show that Plexin B1 is profoundly downregulated by UVB in melanocytes, loss of Plexin B1 may accentuate HGF-dependent effects on melanocytes, including melanocyte migration.

Cyclic GMP-gated CNG channels function in Sema3A-induced growth cone repulsion.

PubMed

Togashi, Kazunobu; von Schimmelmann, Melanie J; Nishiyama, Makoto; Lim, Chae-Seok; Yoshida, Norihiro; Yun, Bokyoung; Molday, Robert S; Goshima, Yoshio; Hong, Kyonsoo

2008-06-12

Cyclic nucleotide-gated channels (CNGCs) transduce external signals required for sensory processes, e.g., photoreception, olfaction, and taste. Nerve growth cone guidance by diffusible attractive and repulsive molecules is regulated by differential growth cone Ca2+ signaling. However, the Ca2+-conducting ion channels that transduce guidance molecule signals are largely unknown. We show that rod-type CNGC-like channels function in the repulsion of cultured Xenopus spinal neuron growth cones by Sema3A, which triggers the production of the cGMP that activates the Xenopus CNGA1 (xCNGA1) subunit-containing channels in interneurons. Downregulation of xCNGA1 or overexpression of a mutant xCNGA1 incapable of binding cGMP abolished CNG currents and converted growth cone repulsion to attraction in response to Sema3A. We also show that Ca2+ entry through xCNGCs is required to mediate the repulsive Sema3A signal. These studies extend our knowledge of the function of CNGCs by demonstrating their requirement for signal transduction in growth cone guidance.

A recognizable type of syndromic short stature with arthrogryposis caused by bi-allelic SEMA3A loss-of-function variants.

PubMed

Baumann, M; Steichen-Gersdorf, E; Krabichler, B; Müller, T; Janecke, A R

2017-07-01

The semaphorins constitute a large family of secreted and membrane-associated proteins that regulate many developmental processes, including neural circuit assembly, bone formation and angiogenesis. Recently, bi-allelic loss-of-function variants in SEMA3A (semaphorin 3A) were identified in a single patient with a particular pattern of multiple congenital anomalies (MCA). Using homozygosity mapping combined with exome sequencing, we identified a homozygous SEMA3A variant causing a premature stop codon in an 8 year old boy with the same pattern of MCA. The phenotype of these patients is characterized by postnatal short stature, skeletal anomalies of the thorax, a minor congenital heart or vascular defect, camptodactyly, micropenis, and variable additional anomalies. Motor development is delayed in both patients, and intellectual development is delayed in one patient. Our observation of a second case supports the notion that bi-allelic mutations in SEMA3A cause an autosomal recessive type of syndromic short stature. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Teaching Literacy Skills to French Minimally Verbal School-Aged Children with Autism Spectrum Disorders with the Serious Game SEMA-TIC: An Exploratory Study

PubMed Central

Serret, Sylvie; Hun, Stéphanie; Thümmler, Susanne; Pierron, Prescillia; Santos, Andreia; Bourgeois, Jérémy; Askenazy, Florence

2017-01-01

Learning to read is very challenging for children with Autism Spectrum Disorders (ASD), but also very important, as it can give them access to new knowledge. This is even more challenging in minimally verbal children, who do not have the verbal abilities to learn through usual methods. To address the learning of literacy skills in French minimally verbal school-aged children with ASD, we designed the serious game SEMA-TIC, which relies on non-verbal cognitive skills and uses specific learning strategies adapted to the features of autistic individuals. This study investigated the usability of SEMA-TIC (in terms of adaptability, efficiency, and effectiveness) for the acquisition of literacy skills in French minimally verbal school-aged children with ASD. Twenty-five children with ASD and no functional language participated in the study. Children in the training group received the SEMA-TIC training over 23 weeks (on average), while no intervention was provided to children in the non-training group. Results indicated that SEMA-TIC presents a suitable usability, as all participants were able to play (adaptability), to complete the training (efficiency) and to acquire significant literacy skills (effectiveness). Indeed, the literacy skills in the training group significantly improved after the training, as measured by specific experimental tasks (alphabet knowledge, word reading, word-non-word discrimination, sentence reading and word segmentation; all p ≤ 0.001) compared to the non-training group. More importantly, 3 out of 12 children of the training group could be considered as word decoders at the end of the intervention, whereas no children of the non-training group became able to decode words efficiently. The present study thus brings preliminary evidence that French minimally verbal school-aged children with ASD are able to learn literacy skills through SEMA-TIC, a specific computerized intervention consisting in a serious game based on non-verbal cognitive skills. PMID:28928701

Effects of mechanical repetitive load on bone quality around implants in rat maxillae

PubMed Central

Uto, Yusuke; Nakano, Takayoshi; Ishimoto, Takuya; Inaba, Nao; Uchida, Yusuke; Sawase, Takashi

2017-01-01

Greater understanding and acceptance of the new concept “bone quality”, which was proposed by the National Institutes of Health and is based on bone cells and collagen fibers, are required. The novel protein Semaphorin3A (Sema3A) is associated with osteoprotection by regulating bone cells. The aims of this study were to investigate the effects of mechanical loads on Sema3A production and bone quality based on bone cells and collagen fibers around implants in rat maxillae. Grade IV-titanium threaded implants were placed at 4 weeks post-extraction in maxillary first molars. Implants received mechanical loads (10 N, 3 Hz for 1800 cycles, 2 days/week) for 5 weeks from 3 weeks post-implant placement to minimize the effects of wound healing processes by implant placement. Bone structures, bone mineral density (BMD), Sema3A production and bone quality based on bone cells and collagen fibers were analyzed using microcomputed tomography, histomorphometry, immunohistomorphometry, polarized light microscopy and birefringence measurement system inside of the first and second thread (designated as thread A and B, respectively), as mechanical stresses are concentrated and differently distributed on the first two threads from the implant neck. Mechanical load significantly increased BMD, but not bone volume around implants. Inside thread B, but not thread A, mechanical load significantly accelerated Sema3A production with increased number of osteoblasts and osteocytes, and enhanced production of both type I and III collagen. Moreover, mechanical load also significantly induced preferential alignment of collagen fibers in the lower flank of thread B. These data demonstrate that mechanical load has different effects on Sema3A production and bone quality based on bone cells and collagen fibers between the inside threads of A and B. Mechanical load-induced Sema3A production may be differentially regulated by the type of bone structure or distinct stress distribution, resulting in control of bone quality around implants in jaw bones. PMID:29244883

Effects of mechanical repetitive load on bone quality around implants in rat maxillae.

PubMed

Uto, Yusuke; Kuroshima, Shinichiro; Nakano, Takayoshi; Ishimoto, Takuya; Inaba, Nao; Uchida, Yusuke; Sawase, Takashi

2017-01-01

Greater understanding and acceptance of the new concept "bone quality", which was proposed by the National Institutes of Health and is based on bone cells and collagen fibers, are required. The novel protein Semaphorin3A (Sema3A) is associated with osteoprotection by regulating bone cells. The aims of this study were to investigate the effects of mechanical loads on Sema3A production and bone quality based on bone cells and collagen fibers around implants in rat maxillae. Grade IV-titanium threaded implants were placed at 4 weeks post-extraction in maxillary first molars. Implants received mechanical loads (10 N, 3 Hz for 1800 cycles, 2 days/week) for 5 weeks from 3 weeks post-implant placement to minimize the effects of wound healing processes by implant placement. Bone structures, bone mineral density (BMD), Sema3A production and bone quality based on bone cells and collagen fibers were analyzed using microcomputed tomography, histomorphometry, immunohistomorphometry, polarized light microscopy and birefringence measurement system inside of the first and second thread (designated as thread A and B, respectively), as mechanical stresses are concentrated and differently distributed on the first two threads from the implant neck. Mechanical load significantly increased BMD, but not bone volume around implants. Inside thread B, but not thread A, mechanical load significantly accelerated Sema3A production with increased number of osteoblasts and osteocytes, and enhanced production of both type I and III collagen. Moreover, mechanical load also significantly induced preferential alignment of collagen fibers in the lower flank of thread B. These data demonstrate that mechanical load has different effects on Sema3A production and bone quality based on bone cells and collagen fibers between the inside threads of A and B. Mechanical load-induced Sema3A production may be differentially regulated by the type of bone structure or distinct stress distribution, resulting in control of bone quality around implants in jaw bones.

Teaching Literacy Skills to French Minimally Verbal School-Aged Children with Autism Spectrum Disorders with the Serious Game SEMA-TIC: An Exploratory Study.

PubMed

Serret, Sylvie; Hun, Stéphanie; Thümmler, Susanne; Pierron, Prescillia; Santos, Andreia; Bourgeois, Jérémy; Askenazy, Florence

2017-01-01

Learning to read is very challenging for children with Autism Spectrum Disorders (ASD), but also very important, as it can give them access to new knowledge. This is even more challenging in minimally verbal children, who do not have the verbal abilities to learn through usual methods. To address the learning of literacy skills in French minimally verbal school-aged children with ASD, we designed the serious game SEMA-TIC, which relies on non-verbal cognitive skills and uses specific learning strategies adapted to the features of autistic individuals. This study investigated the usability of SEMA-TIC (in terms of adaptability, efficiency, and effectiveness) for the acquisition of literacy skills in French minimally verbal school-aged children with ASD. Twenty-five children with ASD and no functional language participated in the study. Children in the training group received the SEMA-TIC training over 23 weeks (on average), while no intervention was provided to children in the non-training group. Results indicated that SEMA-TIC presents a suitable usability, as all participants were able to play (adaptability), to complete the training (efficiency) and to acquire significant literacy skills (effectiveness). Indeed, the literacy skills in the training group significantly improved after the training, as measured by specific experimental tasks (alphabet knowledge, word reading, word-non-word discrimination, sentence reading and word segmentation; all p ≤ 0.001) compared to the non-training group. More importantly, 3 out of 12 children of the training group could be considered as word decoders at the end of the intervention, whereas no children of the non-training group became able to decode words efficiently. The present study thus brings preliminary evidence that French minimally verbal school-aged children with ASD are able to learn literacy skills through SEMA-TIC, a specific computerized intervention consisting in a serious game based on non-verbal cognitive skills.

Semaphorin6A acts as a gate keeper between the central and the peripheral nervous system

PubMed Central

Mauti, Olivier; Domanitskaya, Elena; Andermatt, Irwin; Sadhu, Rejina; Stoeckli, Esther T

2007-01-01

Background During spinal cord development, expression of chicken SEMAPHORIN6A (SEMA6A) is almost exclusively found in the boundary caps at the ventral motor axon exit point and at the dorsal root entry site. The boundary cap cells are derived from a population of late migrating neural crest cells. They form a transient structure at the transition zone between the peripheral nervous system (PNS) and the central nervous system (CNS). Ablation of the boundary cap resulted in emigration of motoneurons from the ventral spinal cord along the ventral roots. Based on its very restricted expression in boundary cap cells, we tested for a role of Sema6A as a gate keeper between the CNS and the PNS. Results Downregulation of Sema6A in boundary cap cells by in ovo RNA interference resulted in motoneurons streaming out of the spinal cord along the ventral roots, and in the failure of dorsal roots to form and segregate properly. PlexinAs interact with class 6 semaphorins and are expressed by both motoneurons and sensory neurons. Knockdown of PlexinA1 reproduced the phenotype seen after loss of Sema6A function both at the ventral motor exit point and at the dorsal root entry site of the lumbosacral spinal cord. Loss of either PlexinA4 or Sema6D function had an effect only at the dorsal root entry site but not at the ventral motor axon exit point. Conclusion Sema6A acts as a gate keeper between the PNS and the CNS both ventrally and dorsally. It is required for the clustering of boundary cap cells at the PNS/CNS interface and, thus, prevents motoneurons from streaming out of the ventral spinal cord. At the dorsal root entry site it organizes the segregation of dorsal roots. PMID:18088409

Grb2 mediates semaphorin-4D-dependent RhoA inactivation.

PubMed

Sun, Tianliang; Krishnan, Rameshkumar; Swiercz, Jakub M

2012-08-01

Signaling through the semaphorin 4D (Sema4D) receptor plexin-B1 is modulated by its interaction with tyrosine kinases ErbB-2 and Met. In cells expressing the plexin-B1-ErbB-2 receptor complex, ligand stimulation results in the activation of small GTPase RhoA and stimulation of cellular migration. By contrast, in cells expressing plexin-B1 and Met, ligand stimulation results in an association with the RhoGTPase-activating protein p190 RhoGAP and subsequent RhoA inactivation--a process that involves the tyrosine phosphorylation of plexin-B1 by Met. Inactivation of RhoA is necessary for Sema4D-mediated inhibition of cellular migration. It is, however, unknown how plexin-B1 phosphorylation regulates RhoGAP interaction and activity. Here we show that the activation of plexin-B1 by Sema4D and its subsequent tyrosine phosphorylation by Met creates a docking site for the SH2 domain of growth factor receptor bound-2 (Grb2). Grb2 is thereby recruited into the plexin-B1 receptor complex and, through its SH3 domain, interacts with p190 RhoGAP and mediates RhoA deactivation. Phosphorylation of plexin-B1 by Met and the recruitment of Grb2 have no effect on the R-RasGAP activity of plexin-B1, but are required for Sema4D-induced, RhoA-dependent antimigratory effects of Sema4D on breast cancer cells. These data show Grb2 as a direct link between plexin and p190-RhoGAP-mediated downstream signaling.

Antagonistic Effects of BACE1 and APH1B-γ-Secretase Control Axonal Guidance by Regulating Growth Cone Collapse.

PubMed

Barão, Soraia; Gärtner, Annette; Leyva-Díaz, Eduardo; Demyanenko, Galina; Munck, Sebastian; Vanhoutvin, Tine; Zhou, Lujia; Schachner, Melitta; López-Bendito, Guillermina; Maness, Patricia F; De Strooper, Bart

2015-09-01

ΒACE1 is the major drug target for Alzheimer's disease, but we know surprisingly little about its normal function in the CNS. Here, we show that this protease is critically involved in semaphorin 3A (Sema3A)-mediated axonal guidance processes in thalamic and hippocampal neurons. An active membrane-bound proteolytic CHL1 fragment is generated by BACE1 upon Sema3A binding. This fragment relays the Sema3A signal via ezrin-radixin-moesin (ERM) proteins to the neuronal cytoskeleton. APH1B-γ-secretase-mediated degradation of this fragment stops the Sema3A-induced collapse and sensitizes the growth cone for the next axonal guidance cue. Thus, we reveal a cycle of proteolytic activity underlying growth cone collapse and restoration used by axons to find their correct trajectory in the brain. Our data also suggest that BACE1 and γ-secretase inhibition have physiologically opposite effects in this process, supporting the idea that combination therapy might attenuate some of the side effects associated with these drugs. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

Semaphorin 3G Provides a Repulsive Guidance Cue to Lymphatic Endothelial Cells via Neuropilin-2/PlexinD1.

PubMed

Liu, Xinyi; Uemura, Akiyoshi; Fukushima, Yoko; Yoshida, Yutaka; Hirashima, Masanori

2016-11-22

The vertebrate circulatory system is composed of closely related blood and lymphatic vessels. It has been shown that lymphatic vascular patterning is regulated by blood vessels during development, but its molecular mechanisms have not been fully elucidated. Here, we show that the artery-derived ligand semaphorin 3G (Sema3G) and the endothelial cell receptor PlexinD1 play a role in lymphatic vascular patterning. In mouse embryonic back skin, genetic inactivation of Sema3G or PlexinD1 results in abnormal artery-lymph alignment and reduced lymphatic vascular branching. Conditional ablation in mice demonstrates that PlexinD1 is primarily required in lymphatic endothelial cells (LECs). In vitro analyses show that Sema3G binds to neuropilin-2 (Nrp2), which forms a receptor complex with PlexinD1. Sema3G induces cell collapse in an Nrp2/PlexinD1-dependent manner. Our findings shed light on a molecular mechanism by which LECs are distributed away from arteries and form a branching network during lymphatic vascular development. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

Structure of the Plexin Ectodomain Bound by Semaphorin-Mimicking Antibodies

PubMed Central

Omiya, Ryusuke; Matoba, Kyoko; Baba, Takeshi; Suzuki, Sachiyo; Segawa, Hiroaki; Kumanogoh, Atsushi; Iwasaki, Kenji; Hattori, Kunihiro; Takagi, Junichi

2016-01-01

Semaphorin family proteins act on cells to mediate both repulsive and attractive guidance via binding to plexin family receptors, thereby playing fundamental roles in the morphogenesis and homeostasis of various tissues. Although semaphorin-plexin signaling is implicated in various diseases and is thus a target of intensive research, our mechanistic understanding of how semaphorins activate plexins on the cell surface is limited. Here, we describe unique anti-plexin-A1 antibodies that can induce a collapsed morphology in mouse dendritic cells as efficiently as the semaphorin 3A (Sema3A) ligand. Precise epitope analysis indicates that these “semaphorin-mimicking” antibodies dimerize cell-surface plexin-A1 by binding to the N-terminal sema domain of the plexin at sites away from the interface used by the Sema3A ligand. Structural analysis of plexin-A1 fragments using negative stain electron microscopy further revealed that this agonistic capacity is closely linked to the location and orientation of antibody binding. In addition, the full-length plexin-A1 ectodomain exhibited a highly curved “C” shape, reinforcing the very unusual dimeric receptor conformation of this protein at the cell surface when engaged with Sema3A or agonistic antibodies. PMID:27258772

[CHANGES OF SEMAPHORIN 3A EXPRESSION IN HEALING OF TIBIA FRACTURE AFTER TRAUMATIC BRAIN INJURY].

PubMed

Li, Zhengzheng; Zhao, Junwei; Yi, Zhigang; Luo, Wei; Li, Kang; Wang, Yuliang; Wang, Jing; An, Liping; Ma, Jinglin

2016-10-08

To investigate the mechanism of Semaphorin 3A (Sema3A) in fracture healing after nerve injury by observing the expression of Sema3A in the tibia fracture healing after traumatic brain injury (TBI). A total of 192 Wistar female rats, 8-10 weeks old and weighing 220-250 g, were randomly divided into tibia fracture group (group A, n =48), TBI group (group B, n =48), TBI with tibia fracture group (group C, n =48), and control group (group D, n =48). The tibia fracture model was established at the right side of group A; TBI model was made in group B by the improved Feeney method; the TBI and tibia fracture model was made in group C; no treatment was given in group D. The tissue samples were respectively collected at 3, 5, 7, 14, 21, and 28 days after operation; HE staining, immunohistochemistry staining, and Western blot method were used for the location and quantitative detection of Sema3A in callus tissue. HE staining showed that no obvious changes were observed at each time point in groups B and D. At 3 and 5 days, there was no obvious callus growth at fracture site with inflammatory cells and fibrous tissue filling in groups A and C. At 7 and 14 days, fibrous tissue grew from periosteum to fracture site in groups A and C; the proliferation of chondrocytes in exterior periosteum gradually formed osteoid callus at fracture site in groups A and C. The chondrocyte had bigger size, looser arrangement, and more osteoid in group C than group A. Group B had disorder periosteum, slight subperiosteal bone hyperplasia, and no obvious change of bone trabecula in group B when compared with group D. At 21 and 28 days, cartilage callus was gradually replaced by new bone trabecula in groups A and C. Group C had loose arrange, disorder structure, and low density of bone trabecula, big callus area and few chondrocyte and osteoid when compared with group A; group B was similar to Group D. Immunohistochemistry staining showed that Sema3A expression in chondrocytes in group C was higher than that in group A, particularly at 7, 14, and 21 day. Sema3A was significantly higher in osteoblasts of new bone trabecula in group A than group C, especially at 14 and 21 days ( P <0.05). Western blot results showed that the Sema3A had the same expression trend during fracture healing in groups A and C. However, the expression of Sema3A protein was significantly higher in group C than group A ( P <0.05) and in group B than group D ( P <0.05) at 7, 14, 21, and 28 days. Abnormal expression of Sema3A may play a role in fracture healing after nerve injury by promoting the chondrocytes proliferation and reducing the distribution of sensory nerve fibers and osteoblast differentiation.

Potential Role of Semaphorin 3A and Its Receptors in Regulating Aberrant Sympathetic Innervation in Peritoneal and Deep Infiltrating Endometriosis

PubMed Central

Liang, Yanchun; Wang, Wei; Huang, Jiaming; Tan, Hao; Liu, Tianyu; Shang, Chunliang; Liu, Duo; Guo, Luyan; Yao, Shuzhong

2015-01-01

Previous studies have demonstrated the involvement of nerve repellent factors in regulation of the imbalanced innervation of endometriosis. This prospective study aims to explore the role of Sema 3A in regulating aberrant sympathetic innervation in peritoneal and deep infiltrating endometriosis. Ectopic endometriotic lesion were collected from patients with peritoneal endometriosis (n = 24) and deep infiltrating endometriosis of uterosacral ligament (n = 20) undergoing surgery for endometriosis. Eutopic endometrial samples were collected from patients with endometriosis (n = 22) or without endometriosis (n = 26). Healthy peritoneum (n = 13) from the lateral pelvic wall and healthy uterosacral ligament (n = 13) were obtained from patients who had no surgical and histological proof of endometriosis during hysterectomy for uterine fibroids. Firstly, we studied the immunostaining of Sema 3A, Plexin A1 and NRP-1 in all the tissues described above. Then we studied the nerve fiber density (NFD) of endometriosis-associated (sympathetic) nerve and para-endometriotic (sympathetic) nerve by double immunofluorescence staining. Finally we analyzed the relationship between expression of Sema 3A in stromal cells of endometriotic lesion and the aberrant innervation of endometriosis. Semi-quantitative immunostaining demonstrated that (1) Higher immunostaining of Sema 3A were found in the eutopic endometrial glandular epithelial cells from patients with endometriosis (p = 0.041) than those without endometriosis; (2) Sema 3A immunostaining was higher in glandular epithelial cells of peritoneal endometriosis (P<0.001) and deep infiltrating endometriotic lesions of uterosacral ligament (P = 0.028)compared with glandular epithelial cells of the endometrium from women with endometriosis, while its expression in ectopic stormal cells in both groups were significantly lower than that from eutopic endometrium of women without endometirosis (P<0.001, P<0.001, respectively). NFDs of Anti-TH (+) endometriosis-associated sympathetic nerve of peritoneal endometriosis (p<0.001) and deep endometriosis of uterosacral ligament (p<0.001) were significantly lower than NFDs of para-endometriotic sympathetic nerve. Our results suggest that Sema 3A may contribute to the regulation of aberrant sympathetic innervation in peritoneal and deep infiltrating endometriosis. PMID:26720585

Recent development of plasma optical systems (invited)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Goncharov, A. A., E-mail: gonchar@iop.kiev.ua

2016-02-15

The article devotes a brief description of the recent development and current status of an ongoing research of plasma optical systems based on the fundamental plasma optical idea magnetic electron isolation, equipotentialization magnetic field lines, and the axi-symmetric cylindrical electrostatic plasma lens (PL) configuration. The experimental, theoretical, and simulation investigations have been carried out over recent years collaboratively between IP NASU (Kiev), LBNL (Berkeley, USA), and HCEI RAS (Tomsk). The crossed electric and magnetic fields inherent the PL configuration that provides the attractive method for establishing a stable plasma discharge at low pressure. Using PL configuration, several high reliability plasmamore » devices were developed. These devices are attractive for many high-tech applications.« less

Rewiring the taste system.

PubMed

Lee, Hojoon; Macpherson, Lindsey J; Parada, Camilo A; Zuker, Charles S; Ryba, Nicholas J P

2017-08-17

In mammals, taste buds typically contain 50-100 tightly packed taste-receptor cells (TRCs), representing all five basic qualities: sweet, sour, bitter, salty and umami. Notably, mature taste cells have life spans of only 5-20 days and, consequently, are constantly replenished by differentiation of taste stem cells. Given the importance of establishing and maintaining appropriate connectivity between TRCs and their partner ganglion neurons (that is, ensuring that a labelled line from sweet TRCs connects to sweet neurons, bitter TRCs to bitter neurons, sour to sour, and so on), we examined how new connections are specified to retain fidelity of signal transmission. Here we show that bitter and sweet TRCs provide instructive signals to bitter and sweet target neurons via different guidance molecules (SEMA3A and SEMA7A). We demonstrate that targeted expression of SEMA3A or SEMA7A in different classes of TRCs produces peripheral taste systems with miswired sweet or bitter cells. Indeed, we engineered mice with bitter neurons that now responded to sweet tastants, sweet neurons that responded to bitter or sweet neurons responding to sour stimuli. Together, these results uncover the basic logic of the wiring of the taste system at the periphery, and illustrate how a labelled-line sensory circuit preserves signalling integrity despite rapid and stochastic turnover of receptor cells.

Rewiring the Taste System

PubMed Central

Lee, Hojoon; Macpherson, Lindsey J.; Parada, Camilo A.; Zuker, Charles S.; Ryba, Nicholas J.P.

2018-01-01

In mammals, taste buds typically contain 50-100 tightly packed taste receptor cells (TRCs) representing all five basic qualities: sweet, sour, bitter, salty and umami1,2. Notably, mature taste cells have life spans of only 5-20 days, and consequently, are constantly replenished by differentiation of taste stem cells3. Given the importance of establishing and maintaining appropriate connectivity between TRCs and their partner ganglion neurons (i.e. ensuring that a labeled line from sweet TRCs connects to sweet neurons, bitter TRCs to bitter neurons, sour to sour, etc.), we examined how new connections are specified to retain fidelity of signal transmission. Our results show that bitter and sweet TRCs provide instructive signals to bitter and sweet target neurons via different guidance molecules (Sema3A and Sema7A)4-6. Here, we demonstrate that targeted expression of Sema3A or Sema7A in different classes of TRCs produce peripheral taste systems with miswired sweet or bitter cells. Indeed, we engineered animals whereby bitter neurons now respond to sweet tastants, sweet neurons respond to bitter, or with sweet neurons responding to sour stimuli. Together, these results uncover the basic logic of the wiring of the taste system at the periphery, and illustrate how a labeled-line sensory circuit preserves signaling integrity despite rapid and stochastic turnover of receptor cells. PMID:28792937

Role of inhibition of osteogenesis function by Sema4D/Plexin-B1 signaling pathway in skeletal fluorosis in vitro.

PubMed

Liu, Xiao-li; Song, Jing; Liu, Ke-jian; Wang, Wen-peng; Xu, Chang; Zhang, Yu-zeng; Liu, Yun

2015-10-01

Skeletal fluorosis is a chronically metabolic bone disease with extensive hyperostosis osteosclerosis caused by long time exposure to fluoride. Skeletal fluorosis brings about a series of abnormal changes of the extremity, such as joint pain, joint stiffness, bone deformity, etc. Differentiation and maturation of osteoblasts were regulated by osteoclasts via Sema4D/Plexin-B1 signaling pathway. Furthermore, the differentiation and maturation of osteoclasts are conducted by osteoblasts via RANKL/RANK/OPG pathway. Both of these processes form a feedback circuit which is a key link in skeletal fluorosis. In this study, an osteoblast-osteoclast co-culture model in vitro was developed to illustrate the mechanism of skeletal fluorosis. With the increase of fluoride concentration, the expression level of Sema4D was decreased and TGF-β1 was increased continuously. OPG/RANKL mRNA level, however, increased gradually. On the basis of that, the inhibition of Sema4D/Plexin-B1/RhoA/ROCK signaling pathway caused by fluoride promoted the level of TGF-β1 and activated the proliferation of osteoblasts. In addition, osteroprotegerin (OPG) secreted by osteoblasts was up-regulated by fluoride. The competitive combination of OPG and RANKL was strengthened and the combination of RANKL and RANK was hindered. And then the differentiation and maturation of osteoclasts were inhibited, and bone absorption was weakened, leading to skeletal fluorosis.

Reversal of gene dysregulation in cultured cytotrophoblasts reveals possible causes of preeclampsia

PubMed Central

Zhou, Yan; Gormley, Matthew J.; Hunkapiller, Nathan M.; Kapidzic, Mirhan; Stolyarov, Yana; Feng, Victoria; Nishida, Masakazu; Drake, Penelope M.; Bianco, Katherine; Wang, Fei; McMaster, Michael T.; Fisher, Susan J.

2013-01-01

During human pregnancy, a subset of placental cytotrophoblasts (CTBs) differentiates into cells that aggressively invade the uterus and its vasculature, anchoring the progeny and rerouting maternal blood to the placenta. In preeclampsia (PE), CTB invasion is limited, reducing placental perfusion and/or creating intermittent flow. This syndrome, affecting 4%–8% of pregnancies, entails maternal vascular alterations (e.g., high blood pressure, proteinuria, and edema) and, in some patients, fetal growth restriction. The only cure is removal of the faulty placenta, i.e., delivery. Previously, we showed that defective CTB differentiation contributes to the placental component of PE, but the causes were unknown. Here, we cultured CTBs isolated from PE and control placentas for 48 hours, enabling differentiation and invasion. In various severe forms of PE, transcriptomics revealed common aberrations in CTB gene expression immediately after isolation, including upregulation of SEMA3B, which resolved in culture. The addition of SEMA3B to normal CTBs inhibited invasion and recreated aspects of the PE phenotype. Additionally, SEMA3B downregulated VEGF signaling through the PI3K/AKT and GSK3 pathways, effects that were observed in PE CTBs. We propose that, in severe PE, the in vivo environment dysregulates CTB gene expression; the autocrine actions of the upregulated molecules (including SEMA3B) impair CTB differentiation, invasion and signaling; and patient-specific factors determine the signs. PMID:23934129

Math Anxiety in Second and Third Graders and Its Relation to Mathematics Achievement

PubMed Central

Wu, Sarah S.; Barth, Maria; Amin, Hitha; Malcarne, Vanessa; Menon, Vinod

2012-01-01

Although the detrimental effects of math anxiety in adults are well understood, few studies have examined how it affects younger children who are beginning to learn math in a formal academic setting. Here, we examine the relationship between math anxiety and math achievement in second and third graders. In response to the need for a grade-appropriate measure of assessing math anxiety in this group we first describe the development of Scale for Early Mathematics Anxiety (SEMA), a new measure for assessing math anxiety in second and third graders that is based on the Math Anxiety Rating Scale. We demonstrate the construct validity and reliability of the SEMA and use it to characterize the effect of math anxiety on standardized measures of math abilities, as assessed using the Mathematical Reasoning and Numerical Operations subtests of the Wechsler Individual Achievement Test (WIAT-II). Math achievement, as measured by the WIAT-II Math Composite score, was significantly and negatively correlated with SEMA but not with trait anxiety scores. Additional analyses showed that SEMA scores were strongly correlated with Mathematical Reasoning scores, which involves more complex verbal problem solving. SEMA scores were weakly correlated with Numerical Operations which assesses basic computation skills, suggesting that math anxiety has a pronounced effect on more demanding calculations. We also found that math anxiety has an equally detrimental impact on math achievement regardless of whether children have an anxiety related to numbers or to the situational and social experience of doing math. Critically, these effects were unrelated to trait anxiety, providing the first evidence that the specific effects of math anxiety can be detected in the earliest stages of formal math learning in school. Our findings provide new insights into the developmental origins of math anxiety, and further underscore the need to remediate math anxiety and its deleterious effects on math achievement in young children. PMID:22701105

Math anxiety in second and third graders and its relation to mathematics achievement.

PubMed

Wu, Sarah S; Barth, Maria; Amin, Hitha; Malcarne, Vanessa; Menon, Vinod

2012-01-01

Although the detrimental effects of math anxiety in adults are well understood, few studies have examined how it affects younger children who are beginning to learn math in a formal academic setting. Here, we examine the relationship between math anxiety and math achievement in second and third graders. In response to the need for a grade-appropriate measure of assessing math anxiety in this group we first describe the development of Scale for Early Mathematics Anxiety (SEMA), a new measure for assessing math anxiety in second and third graders that is based on the Math Anxiety Rating Scale. We demonstrate the construct validity and reliability of the SEMA and use it to characterize the effect of math anxiety on standardized measures of math abilities, as assessed using the Mathematical Reasoning and Numerical Operations subtests of the Wechsler Individual Achievement Test (WIAT-II). Math achievement, as measured by the WIAT-II Math Composite score, was significantly and negatively correlated with SEMA but not with trait anxiety scores. Additional analyses showed that SEMA scores were strongly correlated with Mathematical Reasoning scores, which involves more complex verbal problem solving. SEMA scores were weakly correlated with Numerical Operations which assesses basic computation skills, suggesting that math anxiety has a pronounced effect on more demanding calculations. We also found that math anxiety has an equally detrimental impact on math achievement regardless of whether children have an anxiety related to numbers or to the situational and social experience of doing math. Critically, these effects were unrelated to trait anxiety, providing the first evidence that the specific effects of math anxiety can be detected in the earliest stages of formal math learning in school. Our findings provide new insights into the developmental origins of math anxiety, and further underscore the need to remediate math anxiety and its deleterious effects on math achievement in young children.

Implication of anti-inflammatory macrophages in regenerative moto-neuritogenesis: promotion of myoblast migration and neural chemorepellent semaphorin 3A expression in injured muscle.

PubMed

Sakaguchi, Shohei; Shono, Jun-ichi; Suzuki, Takahiro; Sawano, Shoko; Anderson, Judy E; Do, Mai-Khoi Q; Ohtsubo, Hideaki; Mizunoya, Wataru; Sato, Yusuke; Nakamura, Mako; Furuse, Mitsuhiro; Yamada, Koji; Ikeuchi, Yoshihide; Tatsumi, Ryuichi

2014-09-01

Regenerative mechanisms that regulate intramuscular motor innervation are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed a heretofore unexplored role of resident myogenic stem cell (satellite cell)-derived myoblasts as a key presenter of a secreted neural chemorepellent semaphorin 3A (Sema3A); hepatocyte growth factor (HGF) triggered its expression exclusively at the early-differentiation phase. In order to verify this concept, the present study was designed to clarify a paracrine source of HGF release. In vitro experiments demonstrated that activated anti-inflammatory macrophages (CD206-positive M2) produce HGF and thereby promote myoblast chemoattraction and Sema3A expression. Media from pro-inflammatory macrophage cultures (M1) did not show any significant effect. M2 also enhanced the expression of myoblast-differentiation markers in culture, and infiltrated predominantly at the early-differentiation phase (3-5 days post-injury); M2 were confirmed to produce HGF as monitored by in vivo/ex vivo immunocytochemistry of CD11b/CD206/HGF-positive cells and by HGF in situ hybridization of cardiotoxin- or crush-injured tibialis anterior muscle, respectively. These studies advance our understanding of the stage-specific activation of Sema3A expression signaling. Findings, therefore, encourage the idea that M2 contribute to spatiotemporal up-regulation of extracellular Sema3A concentrations by producing HGF that, in turn, stimulates a burst of Sema3A secretion by myoblasts that are recruited to site of injury. This model may ensure a coordinated delay in re-attachment of motoneuron terminals onto damaged fibers early in muscle regeneration, and thus synchronize the recovery of muscle-fiber integrity and the early resolution of inflammation after injury. Copyright © 2014 Elsevier Ltd. All rights reserved.

LDL receptor-related protein 1 regulates the abundance of diverse cell-signaling proteins in the plasma membrane proteome.

PubMed

Gaultier, Alban; Simon, Gabriel; Niessen, Sherry; Dix, Melissa; Takimoto, Shinako; Cravatt, Benjamin F; Gonias, Steven L

2010-12-03

LDL receptor-related protein 1 (LRP1) is an endocytic receptor, reported to regulate the abundance of other receptors in the plasma membrane, including uPAR and tissue factor. The goal of this study was to identify novel plasma membrane proteins, involved in cell-signaling, that are regulated by LRP1. Membrane protein ectodomains were prepared from RAW 264.7 cells in which LRP1 was silenced and control cells using protease K. Peptides were identified by LC-MS/MS. By analysis of spectral counts, 31 transmembrane and secreted proteins were regulated in abundance at least 2-fold when LRP1 was silenced. Validation studies confirmed that semaphorin4D (Sema4D), plexin domain-containing protein-1 (Plxdc1), and neuropilin-1 were more abundant in the membranes of LRP1 gene-silenced cells. Regulation of Plxdc1 by LRP1 was confirmed in CHO cells, as a second model system. Plxdc1 coimmunoprecipitated with LRP1 from extracts of RAW 264.7 cells and mouse liver. Although Sema4D did not coimmunoprecipitate with LRP1, the cell-surface level of Sema4D was increased by RAP, which binds to LRP1 and inhibits binding of other ligands. These studies identify Plxdc1, Sema4D, and neuropilin-1 as novel LRP1-regulated cell-signaling proteins. Overall, LRP1 emerges as a generalized regulator of the plasma membrane proteome.

Retrograde Semaphorin-Plexin Signaling Drives Homeostatic Synaptic Plasticity

PubMed Central

Orr, Brian O.; Fetter, Richard D.; Davis, Graeme W.

2017-01-01

Homeostatic signaling systems ensure stable, yet flexible neural activity and animal behavior1–4. Defining the underlying molecular mechanisms of neuronal homeostatic signaling will be essential in order to establish clear connections to the causes and progression of neurological disease. Presynaptic homeostatic plasticity (PHP) is a conserved form of neuronal homeostatic signaling, observed in organisms ranging from Drosophila to human1,5. Here, we demonstrate that Semaphorin2b (Sema2b) is target-derived signal that acts upon presynaptic PlexinB (PlexB) receptors to mediate the retrograde, homeostatic control of presynaptic neurotransmitter release at the Drosophila neuromuscular junction. Sema2b-PlexB signaling regulates the expression of PHP via the cytoplasmic protein Mical and the oxoreductase-dependent control of presynaptic actin6,7. During neural development, Semaphorin-Plexin signaling instructs axon guidance and neuronal morphogenesis8–10. Yet, Semaphorins and Plexins are also expressed in the adult brain11–16. Here we demonstrate that Semaphorin-Plexin signaling controls presynaptic neurotransmitter release. We propose that Sema2b-PlexB signaling is an essential platform for the stabilization of synaptic transmission throughout life. PMID:28953869

Neuropilin 2: Novel Biomarker and Therapeutic Target for Aggressive Prostate Cancer

DTIC Science & Technology

2015-09-01

1 (0.01) 6.11 (0.44) NPR2 1 (0.042) 17.7 (1.56) 1 (0.27) 15.08 (1.99) GROWTH FACTOR RECEPTOR STEMNESS C hIgG Beva c-S EM A c-fu rSE MA 0 0.2 0.4 0.6...peptide (c-furSEMA) or control peptide (c-SEMA). 9 :VEGF c-S EM A c-fu rSE MAA C PC 3-S PC 3-R C4 -2S C4 -2R Rac-active Rac-input VEGF PC3 C4-2

Towards Supporting Climate Scientists and Impact Assessment Analysts with the Big Data Europe Platform

NASA Astrophysics Data System (ADS)

Klampanos, Iraklis; Vlachogiannis, Diamando; Andronopoulos, Spyros; Cofiño, Antonio; Charalambidis, Angelos; Lokers, Rob; Konstantopoulos, Stasinos; Karkaletsis, Vangelis

2016-04-01

The EU, Horizon 2020, project Big Data Europe (BDE) aims to support European companies and institutions in effectively managing and making use of big data in activities critical to their progress and success. BDE focuses on seven areas of societal impact: Health, Food and Agriculture, Energy, Transport, Climate, Social Sciences and Security. By reaching out to partners and stakeholders, BDE aims to elicit data-intensive requirements for, and deliver an ICT platform to cover aspects of publishing and consuming semantically interoperable, large-scale, multi-lingual data assets and knowledge. In this presentation we will describe the first BDE pilot for Climate, focusing on SemaGrow, its core component, which provides data querying and management based on data semantics. Over the last few decades, extended scientific effort in understanding climate change has resulted in a huge volume of model and observational data. Large international global and regional model inter-comparison projects have focused on creating a framework in support of climate model diagnosis, validation, documentation and data access. The application of climate model ensembles, a system consisting of different possible realisations of a climate model, has further significantly increased the amount of climate and weather data generated. The provision of such models satisfies the crucial objective of assessing potential impacts of climate change on well-being for adaptation, prevention and mitigation. One of the methodologies applied by the climate research and impact assessment communities is that of dynamical downscaling. This calculates values of atmospheric variables in smaller spatial and temporal scales, given a global model. On the company or institution level, this process can be greatly improved in terms of querying, data ingestion from various sources and formats, automatic data mapping, etc. The first Climate BDE pilot will facilitate the process of dynamical downscaling by providing a semantics-based interface to climate open data, eg{} to ESGF services, searching, downloading and indexing climate model and observational data, according to user requirements, such as coverage and experimental scenarios, executing dynamical downscaling models on institutional computing resources, and establishing a framework for metadata mappings and data lineage. The objectives of this pilot will be met building on the SemaGrow system and tools, which have been developed as part of the SemaGrow project in order to scale data intensive techniques up to extremely large data volumes and improve real time performance for agricultural experiments and analyses. SemaGrow is a query resolution and ingestion system for data and semantics. It is able to extract semantic features from data, index them and expose APIs to other BDE platform components. Moreover, SemaGrow provides tools for transforming and managing data in various formats (e.g. NetCDF), and their metadata. It can also interface between users and distributed, external data sources via SPARQL endpoints. This has been demonstrated as part of the SemaGrow project, on diverse and large-scale scientific use-cases. SemaGrow is an active data service in agINFRA, a data infrastructure for agriculture. https://github.com/semagrow/semagrow Big Data Europe (http://www.big-data-europe.eu) - grant agreement no.644564. Earth System Grid Federation: http://esgf.llnl.gov http://www.semagrow.eu http://aginfra.eu

SEMA3A, a Gene Involved in Axonal Pathfinding, Is Mutated in Patients with Kallmann Syndrome

PubMed Central

Parkash, Jyoti; Espy, Cécile; Fouveaut, Corinne; Leroy, Chrystel; Baron, Stéphanie; Campagne, Céline; Vanacker, Charlotte; Collier, Francis; Cruaud, Corinne; Meyer, Vincent; García-Piñero, Alfons; Dewailly, Didier; Cortet-Rudelli, Christine; Gersak, Ksenija; Metz, Chantal; Chabrier, Gérard; Pugeat, Michel; Young, Jacques; Hardelin, Jean-Pierre; Prevot, Vincent; Dodé, Catherine

2012-01-01

Kallmann syndrome (KS) associates congenital hypogonadism due to gonadotropin-releasing hormone (GnRH) deficiency and anosmia. The genetics of KS involves various modes of transmission, including oligogenic inheritance. Here, we report that Nrp1 sema/sema mutant mice that lack a functional semaphorin-binding domain in neuropilin-1, an obligatory coreceptor of semaphorin-3A, have a KS–like phenotype. Pathohistological analysis of these mice indeed showed abnormal development of the peripheral olfactory system and defective embryonic migration of the neuroendocrine GnRH cells to the basal forebrain, which results in increased mortality of newborn mice and reduced fertility in adults. We thus screened 386 KS patients for the presence of mutations in SEMA3A (by Sanger sequencing of all 17 coding exons and flanking splice sites) and identified nonsynonymous mutations in 24 patients, specifically, a frameshifting small deletion (D538fsX31) and seven different missense mutations (R66W, N153S, I400V, V435I, T688A, R730Q, R733H). All the mutations were found in heterozygous state. Seven mutations resulted in impaired secretion of semaphorin-3A by transfected COS-7 cells (D538fsX31, R66W, V435I) or reduced signaling activity of the secreted protein in the GN11 cell line derived from embryonic GnRH cells (N153S, I400V, T688A, R733H), which strongly suggests that these mutations have a pathogenic effect. Notably, mutations in other KS genes had already been identified, in heterozygous state, in five of these patients. Our findings indicate that semaphorin-3A signaling insufficiency contributes to the pathogenesis of KS and further substantiate the oligogenic pattern of inheritance in this developmental disorder. PMID:22927827

SEMA3A, a gene involved in axonal pathfinding, is mutated in patients with Kallmann syndrome.

PubMed

Hanchate, Naresh Kumar; Giacobini, Paolo; Lhuillier, Pierre; Parkash, Jyoti; Espy, Cécile; Fouveaut, Corinne; Leroy, Chrystel; Baron, Stéphanie; Campagne, Céline; Vanacker, Charlotte; Collier, Francis; Cruaud, Corinne; Meyer, Vincent; García-Piñero, Alfons; Dewailly, Didier; Cortet-Rudelli, Christine; Gersak, Ksenija; Metz, Chantal; Chabrier, Gérard; Pugeat, Michel; Young, Jacques; Hardelin, Jean-Pierre; Prevot, Vincent; Dodé, Catherine

2012-08-01

Kallmann syndrome (KS) associates congenital hypogonadism due to gonadotropin-releasing hormone (GnRH) deficiency and anosmia. The genetics of KS involves various modes of transmission, including oligogenic inheritance. Here, we report that Nrp1(sema/sema) mutant mice that lack a functional semaphorin-binding domain in neuropilin-1, an obligatory coreceptor of semaphorin-3A, have a KS-like phenotype. Pathohistological analysis of these mice indeed showed abnormal development of the peripheral olfactory system and defective embryonic migration of the neuroendocrine GnRH cells to the basal forebrain, which results in increased mortality of newborn mice and reduced fertility in adults. We thus screened 386 KS patients for the presence of mutations in SEMA3A (by Sanger sequencing of all 17 coding exons and flanking splice sites) and identified nonsynonymous mutations in 24 patients, specifically, a frameshifting small deletion (D538fsX31) and seven different missense mutations (R66W, N153S, I400V, V435I, T688A, R730Q, R733H). All the mutations were found in heterozygous state. Seven mutations resulted in impaired secretion of semaphorin-3A by transfected COS-7 cells (D538fsX31, R66W, V435I) or reduced signaling activity of the secreted protein in the GN11 cell line derived from embryonic GnRH cells (N153S, I400V, T688A, R733H), which strongly suggests that these mutations have a pathogenic effect. Notably, mutations in other KS genes had already been identified, in heterozygous state, in five of these patients. Our findings indicate that semaphorin-3A signaling insufficiency contributes to the pathogenesis of KS and further substantiate the oligogenic pattern of inheritance in this developmental disorder.

A Critical Period for Postnatal Adaptive Plasticity in a Model of Motor Axon Miswiring

PubMed Central

Castiblanco-Urbina, Maria A.; Winzeck, Stefan; Sundermeier, Julia; Theis, Fabian J.; Fouad, Karim; Huber, Andrea B.

2015-01-01

The correct wiring of neuronal circuits is of crucial importance for precise neuromuscular functionality. Therefore, guidance cues provide tight spatiotemporal control of axon growth and guidance. Mice lacking the guidance cue Semaphorin 3F (Sema3F) display very specific axon wiring deficits of motor neurons in the medial aspect of the lateral motor column (LMCm). While these deficits have been investigated extensively during embryonic development, it remained unclear how Sema3F mutant mice cope with these errors postnatally. We therefore investigated whether these animals provide a suitable model for the exploration of adaptive plasticity in a system of miswired neuronal circuitry. We show that the embryonically developed wiring deficits in Sema3F mutants persist until adulthood. As a consequence, these mutants display impairments in motor coordination that improve during normal postnatal development, but never reach wildtype levels. These improvements in motor coordination were boosted to wildtype levels by housing the animals in an enriched environment starting at birth. In contrast, a delayed start of enriched environment housing, at 4 weeks after birth, did not similarly affect motor performance of Sema3F mutants. These results, which are corroborated by neuroanatomical analyses, suggest a critical period for adaptive plasticity in neuromuscular circuitry. Interestingly, the formation of perineuronal nets, which are known to close the critical period for plastic changes in other systems, was not altered between the different housing groups. However, we found significant changes in the number of excitatory synapses on limb innervating motor neurons. Thus, we propose that during the early postnatal phase, when perineuronal nets have not yet been formed around spinal motor neurons, housing in enriched environment conditions induces adaptive plasticity in the motor system by the formation of additional synaptic contacts, in order to compensate for coordination deficits. PMID:25874621

Increased urine semaphorin-3A is associated with renal damage in hypertensive patients with chronic kidney disease: a nested case-control study.

PubMed

Viazzi, Francesca; Ramesh, Ganesan; Jayakumar, Calpurnia; Leoncini, Giovanna; Garneri, Debora; Pontremoli, Roberto

2015-06-01

Semaphorins are guidance proteins implicated in several processes such as angiogenesis, organogenesis, cell migration, and cytokine release. Experimental studies showed that semaphorin-3a (SEMA3A) administration induces transient massive proteinuria, podocyte foot process effacement and endothelial cell damage in healthy animals. While SEMA3A signaling has been demonstrated to be mechanistically involved in experimental diabetic glomerulopathy and in acute kidney injury, to date its role in human chronic kidney disease (CKD) has not been investigated. To test the hypothesis that SEMA3A may play a role in human CKD, we performed a cross-sectional, nested, case-control study on 151 matched hypertensive patients with and without CKD. SEMA3A was quantified in the urine (USEMA) by ELISA. Glomerular filtration rate was estimated (eGFR) by the CKD-EPI formula and albuminuria was measured as albumin-to-creatinine ratio (ACR). USEMA levels were positively correlated with urine ACR (p = 0.001) and serum creatinine (p < 0.001). USEMA was higher in patients with both components of renal damage as compared to those with only one and those with normal renal function (p < 0.007 and <0.001, respectively). The presence of increased USEMA levels (i.e. top quartile) entailed a fourfold higher risk of combined renal damage (p < 0.001) and an almost twofold higher risk of macroalbuminuria (p = 0.005) or of reduced eGFR, even adjusting for confounding factors (p = 0.002). USEMA is independently associated with CKD in both diabetic and non diabetic hypertensive patients. Further studies may help clarify the mechanisms underlying this association and possibly the pathogenic changes leading to the development of CKD.

Transgenerational Radiation Epigenetics

DTIC Science & Technology

2014-11-01

Cxcl12, Cyp1b1, Fhit, Mlh1 , Mthfr, Prdm2, Rarb, Rassf1, Rassf2, Sema3b, Slit2, Sfrp1, Tcf21; Genes with Metastatic Potential: Anxa5, Dlg2, Dusp6...Anxa5, Apc, Bcl2, Birc5, Braf, Cadm1, Cdh1, Cdh13, Cdkn2a, Dlc1, Egfr, Erbb2, Erbb3, Hgf, Hras1, Kras, Lck, Mlh1 , Mmp9, Nf1, Nfkb1, Ptgs2, Sema3b...Trp53; Immune response genes: Bcl2, Cadm1, Csf3, Cxcl12, Cxcl13, Irf4, Lck, Mlh1 , Nfkb1, Pax5, Stat1, Stat2, Tgfb1, Tnf, Trp53, Vegfa; and

Funding to Support the Participation of Scientists Engaged in DOE Research in the 2008 AGU Chapman Conference on Biogeophysics. Final Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Slater, Lee D.

2009-05-11

This project provided travel awards for scientists engaged in research relevant to the DOE mission to participate in the American Geophysical Union (AGU) Chapman Conference on Biogeophysics held October 13-16, 2008, in Portland, Maine (http://www.agu.org/meetings/chapman/2008/fcall/). The objective of this Chapman Conference was to bring together geophysicists, biophysicists, geochemists, geomicrobiologists, and environmental microbiologists that are leaders in their field and have a personal interest in exploring this new interdisciplinary field or are conducting multidisciplinary research with potential impact on biogeophysics in order to define the current state of the science, identify the critical questions facing the community and to generate amore » roadmap for establishing biogeophysics as a critical subdiscipline of earth science research. The sixty participants were an international group of academics, graduate students and scientists at government laboratories engaged in biogeophysics related research. Scientists from Europe, Israel and China traveled to engage North American colleagues in this highly focused 3.5 day meeting. The group included an approximately equal mix of microbiologists, biogeochemists and near surface geophysicists. The recipients of the DOE travel awards were [1] Dennis Bazylinski (University of Nevada, Las Vegas), [2] Yuri Gorby (Craig Venter Institute), [3] Carlos Santamarina (Georgia Tech), [4] Susan Hubbard (Lawrence Berkeley Laboratory), [5] Roelof Versteeg (Idaho National Laboratory), [6] Eric Roden (University of Wisconsin), [7] George Luther (University of Delaware), and [8] Jinsong Chen (Lawrence Berkeley Laboratory)« less

The role of Sema3–Npn-1 signaling during diaphragm innervation and muscle development

PubMed Central

Huettl, Rosa-Eva; Hanuschick, Philipp; Amend, Anna-Lena; Alberton, Paolo; Aszodi, Attila; Huber, Andrea B.

2016-01-01

ABSTRACT Correct innervation of the main respiratory muscle in mammals, namely the thoracic diaphragm, is a crucial pre-requisite for the functionality of this muscle and the viability of the entire organism. Systemic impairment of Sema3A–Npn-1 (Npn-1 is also known as NRP1) signaling causes excessive branching of phrenic nerves in the diaphragm and into the central tendon region, where the majority of misguided axons innervate ectopic musculature. To elucidate whether these ectopic muscles are a result of misguidance of myoblast precursors due to the loss of Sema3A–Npn-1 signaling, we conditionally ablated Npn-1 in somatic motor neurons, which led to a similar phenotype of phrenic nerve defasciculation and, intriguingly, also formation of innervated ectopic muscles. We therefore hypothesize that ectopic myocyte fusion is caused by additional factors released by misprojecting growth cones. Slit2 and its Robo receptors are expressed by phrenic motor axons and migrating myoblasts, respectively, during innervation of the diaphragm. In vitro analyses revealed a chemoattractant effect of Slit2 on primary diaphragm myoblasts. Thus, we postulate that factors released by motor neuron growth cones have an influence on the migration properties of myoblasts during establishment of the diaphragm. PMID:27466379

A GENOME-WIDE LINKAGE AND ASSOCIATION SCAN REVEALS NOVEL LOCI FOR AUTISM

PubMed Central

Weiss, Lauren A.; Arking, Dan E.

2009-01-01

Summary Although autism is a highly heritable neurodevelopmental disorder, attempts to identify specific susceptibility genes have thus far met with limited success 1. Genome-wide association studies (GWAS) using half a million or more markers, particularly those with very large sample sizes achieved through meta-analysis, have shown great success in mapping genes for other complex genetic traits (http://www.genome.gov/26525384). Consequently, we initiated a linkage and association mapping study using half a million genome-wide SNPs in a common set of 1,031 multiplex autism families (1,553 affected offspring). We identified regions of suggestive and significant linkage on chromosomes 6q27 and 20p13, respectively. Initial analysis did not yield genome-wide significant associations; however, genotyping of top hits in additional families revealed a SNP on chromosome 5p15 (between SEMA5A and TAS2R1) that was significantly associated with autism (P = 2 × 10−7). We also demonstrated that expression of SEMA5A is reduced in brains from autistic patients, further implicating SEMA5A as an autism susceptibility gene. The linkage regions reported here provide targets for rare variation screening while the discovery of a single novel association demonstrates the action of common variants. PMID:19812673

RNA sequencing to predict response to TNF-α inhibitors reveals possible mechanism for nonresponse in smokers.

PubMed

Cuppen, Bart V J; Rossato, Marzia; Fritsch-Stork, Ruth D E; Concepcion, Arno N; Linn-Rasker, Suzanne P; Bijlsma, Johannes W J; van Laar, Jacob M; Lafeber, Floris P J G; Radstake, Timothy R

2018-06-06

Several studies have employed microarray-based profiling to predict response to tumor necrosis factor-alpha inhibitors (TNFi) in rheumatoid arthritis (RA); yet efforts to validate these targets have failed to show predictive abilities acceptable for clinical practice. The eighty most extreme responders and nonresponders to TNFi therapy were selected from the observational BiOCURA cohort. RNA sequencing was performed on mRNA from peripheral blood mononuclear cells (PBMCs) collected before initiation of treatment. The expression of pathways as well as individual gene transcripts between responders and nonresponders was investigated. Promising targets were technically replicated and validated in n = 40 new patients using qPCR assays. Before therapy initiation, nonresponders had lower expression of pathways related to interferon and cytokine signaling, while also showing higher levels of two genes, GPR15 and SEMA6B (p = 0.02). The two targets could be validated, however, additional analyses revealed that GPR15 and SEMA6B did not independently predict response, but were rather dose-dependent markers of smoking (p < 0.0001). The study did not identify new transcripts ready to use in clinical practice, yet GPR15 and SEMA6B were recognized as candidate explanatory markers for the reduced treatment success in RA smokers.

Constitutional downregulation of SEMA5A expression in autism.

PubMed

Melin, M; Carlsson, B; Anckarsater, H; Rastam, M; Betancur, C; Isaksson, A; Gillberg, C; Dahl, N

2006-01-01

There is strong evidence for the importance of genetic factors in idiopathic autism. The results from independent twin and family studies suggest that the disorder is caused by the action of several genes, possibly acting epistatically. We have used cDNA microarray technology for the identification of constitutional changes in the gene expression profile associated with idiopathic autism. Samples were obtained and analyzed from 6 affected subjects belonging to multiplex autism families and from 6 healthy controls. We assessed the expression levels for approximately 7,700 genes by cDNA microarrays using mRNA derived from Epstein-Barr virus-transformed B lymphocytes. The microarray data were analyzed in order to identify up- or downregulation of specific genes. A common pattern with nine downregulated genes was identified among samples derived from individuals with autism when compared to controls. Four of these nine genes encode proteins involved in biological processes associated with brain function or the immune system, and are consequently considered as candidates for genes associated with autism. Quantitative real-time PCR confirms the downregulation of the gene encoding SEMA5A, a protein involved in axonal guidance. Epstein-Barr virus should be considered as a possible source for altered expression, but our consistent results make us suggest SEMA5A as a candidate gene in the etiology of idiopathic autism.

Constitutional downregulation of SEMA5A expression in autism

PubMed Central

Melin, Malin; Carlsson, Birgit; Anckarsäter, Henrik; Rastam, Maria; Betancur, Catalina; Isaksson, Anders; Gillberg, Christopher; Dahl, Niklas

2006-01-01

There is strong evidence for the importance of genetic factors in idiopathic autism. The results from independent twin and family studies suggest that the disorder is caused by the action of several genes, possibly acting epistatically. We have used cDNA microarray technology for the identification of constitutional changes in the gene expression profile associated with idiopathic autism. Samples were obtained and analyzed from six affected subjects belonging to multiplex autism families and from six healthy controls. We assessed the expression levels for approximately 7,700 genes by cDNA microarrays using mRNA derived from Epstein Barr virus (EBV)-transformed B-lymphocytes. The microarray data was analyzed in order to identify up- or down-regulation of specific genes. A common pattern with nine down-regulated genes was identified among samples derived from individuals with autism when compared to controls. Four of these nine genes encode proteins involved in biological processes associated with brain function or the immune system, and are consequently considered as candidates for genes associated with autism. Quantitative realtime PCR confirms the down-regulation of the gene encoding SEMA5A, a protein involved in axonal guidance. EBV should be considered as a possible source for altered expression but our consistent results make us suggest SEMA5A a candidate gene in the etiology of idiopathic autism. PMID:17028446

[Supporting patients in self-management: moving to a personalised approach].

PubMed

Eikelenboom, N; van Lieshout, J; Jacobs, A; Verhulst, F; Lacroix, J; van Halteren, A; Klomp, M; Smeele, I; Wensing, M

2016-01-01

The aim of this research was to assess the effect of providing personalised self-management support on patient activation (knowledge, skills, self-efficacy) and self-management behaviour. Cluster randomised trial in 15 general practices (Dutch Trial Register No.: NTR 3960). Patients aged 18 years or older with a chronic condition were invited to participate in the study. The Self-Management Screening (SeMaS) questionnaire - which illustrates barriers to self-management - was used as a tool for personalised self-management support. Nurse practitioners in the intervention practices were trained for 2 hours in using SeMaS and personalising self-management support on the basis of the SeMaS profile. At baseline and after 6 months, patients filled in questionnaires on patient activation (PAM-13) and lifestyle. Using data from the questionnaires and medical records, the use of individual care plans, referrals to self-management interventions, self-monitoring and healthcare use were assessed. We used a multiple multilevel regression model for data analysis. After 6 months, no difference was found in patient activation between the control group (n = 348) and the intervention group (n = 296). 29.4% of the patients in the intervention group performed self-monitoring, versus 15.2% in the control group (regression coefficient r = 0.9, p = 0.01). In the per-protocol analysis (control n = 348; intervention n = 136), the number of individual care plans (r = 1.3, p = 0.04) and the number of patients performing self-monitoring (r = 1.0; p = 0.01) were higher in the intervention group. Personalised self-management support with the use of the SeMaS method stimulates self-monitoring and the use of individual care plans. The intervention had no effect on patient activation or lifestyle. Given the positive secondary outcomes, the further potential of the tool should be researched.

Role of Rho-mediated ROCK-Semaphorin3A signaling pathway in the pathogenesis of Parkinson's disease in a mouse model.

PubMed

Qi, Li; Tang, Yong-Gang; Wang, Lin; He, Wei; Pan, Hong-Hua; Nie, Rong-Rong; Can, Yan

2016-11-15

The present study aims to elucidate the role of Rho-mediated ROCK-Semaphorin3A signaling pathway in the pathogenesis of Parkinson's disease (PD) in a mouse model. One-hundred twelve eight-week male C57BL/6 mice were selected. The mouse model of PD was constructed by intraperitoneal injection of MPTP. All mice were divided into four groups (28 mice in each group): Blank group, Model group, Rho knockout (Rho+/-) group and ROCK knockout (ROCK+/-) group. Changes of behavior of the mice were studied through automatic moving test and rotarod test. Immunohistochemistry (IHC) was used to detect the expressions of TH, CD11b and GFAP. High performance liquid chromatograph (HPLC) was performed for detection of dopamine and its metabolic product. The mRNA and protein expressions of Rho, ROCK, Sema3A, PlexinA and NRP-1 were detected using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. Rho and ROCK knockout improved the damage caused by MPTP on the behavior of mice and protected dopaminergic neurons from injury, along with the increases of dopamine and its metabolic product. The mRNA and protein expressions of Rho, ROCK, Sema3A, PlexinA and NRP-1 were increased in PD mice in the Model group compared with those in the Blank group. Compared to the Model group, the mRNA and protein expressions of Rho, ROCK, Sema3A, PlexinA and NRP-1 were reduced in the Rho+/- and ROCK+/- groups. These findings indicate that Rho and ROCK knockout may improve the behavior of mice and prevent MPTP-induced dopaminergic neurons damage by regulating Sema3A, PlexinA and NRP-1 in a mouse model of PD. Copyright © 2016 Elsevier B.V. All rights reserved.

The neurovascular relation in oxygen-induced retinopathy.

PubMed

Akula, James D; Mocko, Julie A; Benador, Ilan Y; Hansen, Ronald M; Favazza, Tara L; Vyhovsky, Tanya C; Fulton, Anne B

2008-01-01

Longitudinal studies in rat models of retinopathy of prematurity (ROP) have demonstrated that abnormalities of retinal vasculature and function change hand-in-hand. In the developing retina, vascular and neural structures are under cooperative molecular control. In this study of rats with oxygen-induced retinopathy (OIR) models of ROP, mRNA expression of vascular endothelial growth factor (VEGF), semaphorin (Sema), and their neuropilin receptor (NRP) were examined during the course of retinopathy to evaluate their roles in the observed neurovascular congruency. Oxygen exposures designed to induce retinopathy were delivered to Sprague-Dawley rat pups (n=36) from postnatal day (P) 0 to P14 or from P7 to P14. Room-air-reared controls (n=18) were also studied. Sensitivities of the rod photoreceptors (S(rod)) and the postreceptor cells (Sm) were derived from electroretinographic (ERG) records. Arteriolar tortuosity, T(A), was derived from digital fundus images using Retinal Image multi-Scale Analysis (RISA) image analysis software. mRNA expression of VEGF(164), semaphorin IIIA (Sema3A), and neuropilin-1 (NRP-1) was evaluated by RT-PCR of retinal extracts. Tests were performed at P15-P16, P18-P19, and P25-P26. Relations among ERG, RISA, and PCR parameters were evaluated using linear regression on log transformed data. Sm was low and T(A) was high at young ages, then both resolved by P25-P26. VEGF(164) and Sema3A mRNA expression were also elevated early and decreased with age. Low Sm was significantly associated with high VEGF(164) and Sema3A expression. Low S(rod) was also significantly associated with high VEGF(164). S(rod) and Sm were both correlated with T(A). NRP-1 expression was little affected by OIR. The postreceptor retina appears to mediate the vascular abnormalities that characterize OIR. Because of the relationships revealed by these data, early treatment that targets the neural retina may mitigate the effects of ROP.

The neurovascular relation in oxygen-induced retinopathy

PubMed Central

Akula, James D.; Mocko, Julie A.; Benador, Ilan Y.; Hansen, Ronald M.; Favazza, Tara L.; Vyhovsky, Tanya C.

2008-01-01

Purpose Longitudinal studies in rat models of retinopathy of prematurity (ROP) have demonstrated that abnormalities of retinal vasculature and function change hand-in-hand. In the developing retina, vascular and neural structures are under cooperative molecular control. In this study of rats with oxygen-induced retinopathy (OIR) models of ROP, mRNA expression of vascular endothelial growth factor (VEGF), semaphorin (Sema), and their neuropilin receptor (NRP) were examined during the course of retinopathy to evaluate their roles in the observed neurovascular congruency. Methods Oxygen exposures designed to induce retinopathy were delivered to Sprague-Dawley rat pups (n=36) from postnatal day (P) 0 to P14 or from P7 to P14. Room-air-reared controls (n=18) were also studied. Sensitivities of the rod photoreceptors (Srod) and the postreceptor cells (Sm) were derived from electroretinographic (ERG) records. Arteriolar tortuosity, TA, was derived from digital fundus images using Retinal Image multi-Scale Analysis (RISA) image analysis software. mRNA expression of VEGF164, semaphorin IIIA (Sema3A), and neuropilin-1 (NRP-1) was evaluated by RT–PCR of retinal extracts. Tests were performed at P15–P16, P18–P19, and P25–P26. Relations among ERG, RISA, and PCR parameters were evaluated using linear regression on log transformed data. Results Sm was low and TA was high at young ages, then both resolved by P25–P26. VEGF164 and Sema3A mRNA expression were also elevated early and decreased with age. Low Sm was significantly associated with high VEGF164 and Sema3A expression. Low Srod was also significantly associated with high VEGF164. Srod and Sm were both correlated with TA. NRP-1 expression was little affected by OIR. Conclusions The postreceptor retina appears to mediate the vascular abnormalities that characterize OIR. Because of the relationships revealed by these data, early treatment that targets the neural retina may mitigate the effects of ROP. PMID:19112532

GRID INDEPENDENT FUEL CELL OPERATED SMART HOME

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dr. Mohammad S. Alam

2003-12-07

A fuel cell power plant, which utilizes a smart energy management and control (SEMaC) system, supplying the power need of laboratory based ''home'' has been purchased and installed. The ''home'' consists of two rooms, each approximately 250 sq. ft. Every appliance and power outlet is under the control of a host computer, running the SEMaC software package. It is possible to override the computer, in the event that an appliance or power outage is required. Detailed analysis and simulation of the fuel cell operated smart home has been performed. Two journal papers has been accepted for publication and another journalmore » paper is under review. Three theses have been completed and three additional theses are in progress.« less

Positional Cues in the Drosophila Nerve Cord: Semaphorins Pattern the Dorso-Ventral Axis

PubMed Central

Zlatic, Marta; Li, Feng; Strigini, Maura; Grueber, Wesley; Bate, Michael

2009-01-01

During the development of neural circuitry, neurons of different kinds establish specific synaptic connections by selecting appropriate targets from large numbers of alternatives. The range of alternative targets is reduced by well organised patterns of growth, termination, and branching that deliver the terminals of appropriate pre- and postsynaptic partners to restricted volumes of the developing nervous system. We use the axons of embryonic Drosophila sensory neurons as a model system in which to study the way in which growing neurons are guided to terminate in specific volumes of the developing nervous system. The mediolateral positions of sensory arbors are controlled by the response of Robo receptors to a Slit gradient. Here we make a genetic analysis of factors regulating position in the dorso-ventral axis. We find that dorso-ventral layers of neuropile contain different levels and combinations of Semaphorins. We demonstrate the existence of a central to dorsal and central to ventral gradient of Sema 2a, perpendicular to the Slit gradient. We show that a combination of Plexin A (Plex A) and Plexin B (Plex B) receptors specifies the ventral projection of sensory neurons by responding to high concentrations of Semaphorin 1a (Sema 1a) and Semaphorin 2a (Sema 2a). Together our findings support the idea that axons are delivered to particular regions of the neuropile by their responses to systems of positional cues in each dimension. PMID:19547742

The human cumulus--oocyte complex gene-expression profile

PubMed Central

Assou, Said; Anahory, Tal; Pantesco, Véronique; Le Carrour, Tanguy; Pellestor, Franck; Klein, Bernard; Reyftmann, Lionel; Dechaud, Hervé; De Vos, John; Hamamah, Samir

2006-01-01

BACKGROUND The understanding of the mechanisms regulating human oocyte maturation is still rudimentary. We have identified transcripts differentially expressed between immature and mature oocytes, and cumulus cells. METHODS Using oligonucleotides microarrays, genome wide gene expression was studied in pooled immature and mature oocytes or cumulus cells from patients who underwent IVF. RESULTS In addition to known genes such as DAZL, BMP15 or GDF9, oocytes upregulated 1514 genes. We show that PTTG3 and AURKC are respectively the securin and the Aurora kinase preferentially expressed during oocyte meiosis. Strikingly, oocytes overexpressed previously unreported growth factors such as TNFSF13/APRIL, FGF9, FGF14, and IL4, and transcription factors including OTX2, SOX15 and SOX30. Conversely, cumulus cells, in addition to known genes such as LHCGR or BMPR2, overexpressed cell-tocell signaling genes including TNFSF11/RANKL, numerous complement components, semaphorins (SEMA3A, SEMA6A, SEMA6D) and CD genes such as CD200. We also identified 52 genes progressively increasing during oocyte maturation, comprising CDC25A and SOCS7. CONCLUSION The identification of genes up and down regulated during oocyte maturation greatly improves our understanding of oocyte biology and will provide new markers that signal viable and competent oocytes. Furthermore, genes found expressed in cumulus cells are potential markers of granulosa cell tumors. PMID:16571642

Sema4D, the ligand for Plexin B1, suppresses c-Met activation and migration and promotes melanocyte survival and growth

PubMed Central

Soong, Joanne; Chen, Yulin; Shustef, Elina; Scott, Glynis

2011-01-01

Semaphorins are secreted and membrane bound proteins involved in neural pathfinding, organogenesis, and tumor progression, through Plexin and neuropilins receptors. We recently reported that Plexin B1, the Semaphorin 4D receptor, is a tumor suppressor protein for melanoma, in part, through inhibition of the oncogenic c-Met tyrosine kinase receptor. In this report we show that Sema4D is a protective paracrine factor for normal human melanocyte survival in response to ultraviolet irradiation, that it stimulates proliferation, and regulates the activity of the c-Met receptor. c-Met receptor signaling stimulates melanocyte migration, in part through down-regulation of the cell adhesion molecule E-cadherin. Sema4D suppressed activation of c-Met in response to its ligand hepatocyte growth factor (HGF), and partially blocked the suppressive effects of HGF on E-cadherin expression in melanocytes and HGF-dependent migration. These data demonstrate a role for Plexin B1 in maintenance of melanocyte survival and proliferation in the skin, and suggest that Semaphorin 4D and Plexin B1 act cooperatively with HGF and c-Met to regulate c-Met dependent effects in human melanocytes. Because our data show that Plexin B1 is profoundly down-regulated by UVB in melanocytes, loss of Plexin B1 may accentuate HGF dependent effects on melanocytes, including melanocyte migration. PMID:22189792

Naringin ameliorates bone loss induced by sciatic neurectomy and increases Semaphorin 3A expression in denervated bone.

PubMed

Ma, Xinlong; Lv, Jianwei; Sun, Xiaolei; Ma, Jianxiong; Xing, Guosheng; Wang, Ying; Sun, Lei; Wang, Jianbao; Li, Fengbo; Li, Yanjun; Zhao, Zhihu

2016-04-25

Naringin maintains bone mass in various osteoporosis models, while its effect on bone in disuse osteoporosis has not been reported. The present study explores whether naringin can prevent disuse osteoporosis induced by unilateral sciatic neurectomy (USN) and whether the Semaphorin 3A-induced Wnt/β-catenin signalling pathway is involved in the osteoprotection of naringin. Naringin dose-dependently prevented the deterioration of bone mineral density (BMD), trabecular structure and biomechanical strength in femur due to USN. Naringin increased bone formation but inhibited resorption, as indicated by bone-turnover markers in blood and urine and the histological staining of Osteocalcin (OCN) and tartrate-resistant acid phosphatase (TRAP) in femur. Semaphorin 3A (Sema3A) and active β-catenin protein decreased after USN and could be restored by naringin to the levels of the sham-operated rats. In addition, naringin in vitro promoted the differentiation of osteoblasts and inhibited osteoclastic differentiation. Our studies suggest that the down-regulation of Sema3A and the subsequent inactivation of Wnt/β-catenin signalling may be some of the mechanisms involved in USN-induced osteoporosis. Naringin could increase the expression of Sema3A and the activation of Wnt/β-catenin signalling to prevent disuse osteoporosis induced by denervation. Thus, naringin functions in bone maintenance and could be a promising therapeutic alternative in preventing disuse osteoporosis.

Naringin ameliorates bone loss induced by sciatic neurectomy and increases Semaphorin 3A expression in denervated bone

PubMed Central

Ma, Xinlong; Lv, Jianwei; Sun, Xiaolei; Ma, Jianxiong; Xing, Guosheng; Wang, Ying; Sun, Lei; Wang, Jianbao; Li, Fengbo; Li, Yanjun; Zhao, Zhihu

2016-01-01

Naringin maintains bone mass in various osteoporosis models, while its effect on bone in disuse osteoporosis has not been reported. The present study explores whether naringin can prevent disuse osteoporosis induced by unilateral sciatic neurectomy (USN) and whether the Semaphorin 3A-induced Wnt/β-catenin signalling pathway is involved in the osteoprotection of naringin. Naringin dose-dependently prevented the deterioration of bone mineral density (BMD), trabecular structure and biomechanical strength in femur due to USN. Naringin increased bone formation but inhibited resorption, as indicated by bone-turnover markers in blood and urine and the histological staining of Osteocalcin (OCN) and tartrate-resistant acid phosphatase (TRAP) in femur. Semaphorin 3A (Sema3A) and active β-catenin protein decreased after USN and could be restored by naringin to the levels of the sham-operated rats. In addition, naringin in vitro promoted the differentiation of osteoblasts and inhibited osteoclastic differentiation. Our studies suggest that the down-regulation of Sema3A and the subsequent inactivation of Wnt/β-catenin signalling may be some of the mechanisms involved in USN-induced osteoporosis. Naringin could increase the expression of Sema3A and the activation of Wnt/β-catenin signalling to prevent disuse osteoporosis induced by denervation. Thus, naringin functions in bone maintenance and could be a promising therapeutic alternative in preventing disuse osteoporosis. PMID:27109829

A systematic expression analysis implicates Plexin-B2 and its ligand Sema4C in the regulation of the vascular and endocrine system.

PubMed

Zielonka, Matthias; Xia, Jingjing; Friedel, Roland H; Offermanns, Stefan; Worzfeld, Thomas

2010-09-10

Plexins serve as receptors for semaphorins and play important roles in the developing nervous system. Plexin-B2 controls decisive developmental programs in the neural tube and cerebellum. However, whether Plexin-B2 also regulates biological functions in adult nonneuronal tissues is unknown. Here we show by two methodologically independent approaches that Plexin-B2 is expressed in discrete cell types of several nonneuronal tissues in the adult mouse. In the vasculature, Plexin-B2 is selectively expressed in functionally specialized endothelial cells. In endocrine organs, Plexin-B2 localizes to the pancreatic islets of Langerhans and to both cortex and medulla of the adrenal gland. Plexin-B2 expression is also detected in certain types of immune and epithelial cells. In addition, we report on a systematic comparison of the expression patterns of Plexin-B2 and its ligand Sema4C, which show complementarity or overlap in some but not all tissues. Furthermore, we demonstrate that Plexin-B2 and its family member Plexin-B1 display largely nonredundant expression patterns. This work establishes Plexin-B2 and Sema4C as potential regulators of the vascular and endocrine system and provides an anatomical basis to understand the biological functions of this ligand-receptor pair. Copyright 2010 Elsevier Inc. All rights reserved.

76 FR 37650 - Safety Zone; 4th of July Festival Berkeley Marina Fireworks Display Berkeley, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-28

...-AA00 Safety Zone; 4th of July Festival Berkeley Marina Fireworks Display Berkeley, CA AGENCY: Coast... the 4th of July Festival Berkeley Marina Fireworks Display. Unauthorized persons or vessels are... display. Background and Purpose The City of Berkeley Marina will sponsor the 4th of July Festival Berkeley...

77 FR 37604 - Safety Zone; Fourth of July Fireworks, Berkeley Marina, Berkeley, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-22

...: The Coast Guard will enforce a 1,000 foot safety zone around the Berkeley Pier in position 37[deg]51... Zone; Fourth of July Fireworks, Berkeley Marina, Berkeley, CA AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The Coast Guard will enforce the safety zone for the Berkeley...

Mixed-Dimensionality VLSI-Type Configurable Tools for Virtual Prototyping of Biomicrofluidic Devices and Integrated Systems

NASA Astrophysics Data System (ADS)

Makhijani, Vinod B.; Przekwas, Andrzej J.

2002-10-01

This report presents results of a DARPA/MTO Composite CAD Project aimed to develop a comprehensive microsystem CAD environment, CFD-ACE+ Multiphysics, for bio and microfluidic devices and complete microsystems. The project began in July 1998, and was a three-year team effort between CFD Research Corporation, California Institute of Technology (CalTech), University of California, Berkeley (UCB), and Tanner Research, with Mr. Don Verlee from Abbott Labs participating as a consultant on the project. The overall objective of this project was to develop, validate and demonstrate several applications of a user-configurable VLSI-type mixed-dimensionality software tool for design of biomicrofluidics devices and integrated systems. The developed tool would provide high fidelity 3-D multiphysics modeling capability, l-D fluidic circuits modeling, and SPICE interface for system level simulations, and mixed-dimensionality design. It would combine tools for layouts and process fabrication, geometric modeling, and automated grid generation, and interfaces to EDA tools (e.g. Cadence) and MCAD tools (e.g. ProE).

Influence of glutamate-evoked pain and sustained elevated muscle activity on blood oxygenation in the human masseter muscle.

PubMed

Suzuki, Shunichi; Arima, Taro; Kitagawa, Yoshimasa; Svensson, Peter; Castrillon, Eduardo

2017-12-01

This study aimed to investigate the effect of glutamate-evoked masseter muscle pain on intramuscular oxygenation during rest and sustained elevated muscle activity (SEMA). Seventeen healthy individuals participated in two sessions in which they were injected with glutamate and saline in random order. Each session was divided into three, 10-min periods. During the first (period 1) and the last (period 3) 10-min periods, participants performed five intercalated 1-min bouts of masseter SEMA with 1-min periods of 'rest'. At onset of the second 10-min period, glutamate (0.5 ml, 1 M; Ajinomoto, Tokyo, Japan) or isotonic saline (0.5 ml; 0.9%) was injected into the masseter muscle and the participants kept the muscle relaxed in a resting position for 10 min (period 2). The hemodynamic characteristics of the masseter muscle were recorded simultaneously during the experiment by a laser blood-oxygenation monitor. The results demonstrated that glutamate injections caused significant levels of self-reported pain in the masseter muscle; however, this nociceptive input did not have robust effects on intramuscular oxygenation during rest or SEMA tasks. Interestingly, these findings suggest an uncoupling between acute nociceptive activity and hemodynamic parameters in both resting and low-level active jaw muscles. Further studies are needed to explore the pathophysiological significance of blood-flow changes for persistent jaw-muscle pain conditions. © 2017 Eur J Oral Sci.

Plexin-A4–semaphorin 3A signaling is required for Toll-like receptor– and sepsis-induced cytokine storm

PubMed Central

Wen, Haitao; Lei, Yu; Eun, So-Young

2010-01-01

Plexins and semaphorins are ligand–receptor pairs that serve as guidance molecules in the nervous system and play some roles in immunity. Plexins are similar to the Toll-like receptors (TLRs) in their evolutionary conservation from flies to mammals. By studying plexin-A4–deficient (Plxna4−/−) innate immune cells, in this study we show a novel influence of plexin-A4 on TLR signaling. Plxna4−/− cells exhibit defective inflammatory cytokine production upon activation by a spectrum of TLR agonists and bacteria. Plexin-A4 is required for TLR-induced activation of the small guanosine triphosphate hydrolase (GTPase) Rac1 (ras-related C3 botulinum toxin substrate 1). Rac1 activation is accompanied by JNK (c-Jun N-terminal kinase) and NF-κB activation, culminating in TLR-induced binding of NF-κB and AP-1 to the promoters of inflammatory cytokines. Plxna4−/− mice are remarkably resistant to TLR agonist–induced inflammation and polymicrobial peritonitis caused by cecal ligation and puncture. Administration of a ligand of plexin-A4, Sema3A (semaphorin 3A), exacerbates the cytokine storm caused by TLR agonists and bacterial sepsis. TLR engagement can induce Sema3A expression, thus completing an autocrine loop. These findings expand the role of plexins to TLR signaling and suggest plexin-A4 and Sema3A as new intervention points for treating sepsis. PMID:21098092

Oxycodone Self-Administration Induces Alterations in Expression of Integrin, Semaphorin and Ephrin Genes in the Mouse Striatum.

PubMed

Yuferov, Vadim; Zhang, Yong; Liang, Yupu; Zhao, Connie; Randesi, Matthew; Kreek, Mary J

2018-01-01

Oxycodone is one a commonly used medication for pain, and is also a widely abused prescription opioid, like other short-acting MOPr agonists. Neurochemical and structural adaptations in brain following chronic MOPr-agonist administration are thought to underlie pathogenesis and persistence of opiate addiction. Many axon guidance molecules, such as integrins, semaphorins, and ephrins may contribute to oxycodone-induced neuroadaptations through alterations in axon-target connections and synaptogenesis, that may be implicated in the behaviors associated with opiate addiction. However, little is known about this important area. The aim of this study is to investigate alterations in expression of selected integrin, semaphorin, ephrins, netrin, and slit genes in the nucleus accumbens (NAc) and caudate putamen (CPu) of mice following extended 14-day oxycodone self-administration (SA), using RNAseq. Methods: Total RNA from the NAc and CPu were isolated from adult male C57BL/6J mice within 1 h after the last session of oxycodone in a 14-day self-administration paradigm (4h/day, 0.25 mg/kg/infusion, FR1) or from yoked saline controls. Gene expressions were examined using RNA sequencing (RNA-Seq) technology. RNA-Seq libraries were prepared using Illumina's TruSeq® Stranded Total RNA LT kit. The reads were aligned to the mouse reference genome (version mm10) using STAR. DESeq2 was applied to the counts of protein coding genes to estimate the fold change between the treatment groups. False Discovery Rate (FDR) q < 0.1 were used to select genes that have a significant expression change. For selection of a subset of genes related to axon guidance pathway, REACTOME was used. Results: Among 38 known genes of the integrin, semaphorin, and ephrin gene families, RNA-seq data revealed up-regulation of six genes in the NAc: heterodimer receptor, integrins Itgal, Itgb2 , and Itgam , and its ligand semaphorin Sema7a , two semaphorin receptors, plexins Plxnd1 and Plxdc1 . There was down-regulation of eight genes in this region: two integrin genes Itga3 and Itgb8 , semaphorins Sema3c, Sema4g, Sema6a, Sema6d , semaphorin receptor neuropilin Nrp2 , and ephrin receptor Epha3 . In the CPu, there were five differentially expressed axon guidance genes: up-regulation of three integrin genes, Itgal, Itgb2, Itga1 , and down-regulation of Itga9 and ephrin Efna3 were thus observed. No significant alterations in expression of Netrin-1 or Slit were observed. Conclusion: We provide evidence for alterations in the expression of selective axon guidance genes in adult mouse brain following chronic self-administration of oxycodone. Further examination of oxycodone-induced changes in the expression of these specific axon guidance molecules and integrin genes in relation to behavior may provide new insights into development of addiction to oxycodone.

Berkeley Lab Training

Science.gov Websites

Berkeley Lab Berkeley Lab A-Z Index Phone Book Jobs Search DOE Help Berkeley Lab Training Welcome Welcome to Berkeley Lab Training! Login to access your LBNL Training Profile. This provides quick access to all of the courses you need. Look below, to learn about different types of training available at

miR-25-3p reverses epithelial-mesenchymal transition via targeting Sema4C in cisplatin-resistance cervical cancer cells.

PubMed

Song, Jing; Li, Yue

2017-01-01

Acquisition of epithelial-mesenchymal transition (EMT) has recently been proposed as an important contributor of drug resistance in cervical cancer cells. However, the underlying mechanisms are still unclear. MicroRNAs play a crucial role in regulating EMT. The aim of this study was to explore the potential role of miR-25-3p in regulating EMT in cisplatin-resistant (CR) cervical cancer cells. To this end, we established stable CR cervical cancer cells, HeLa-CR and CaSki-CR, and investigated the function of miR-25-3p in regulating EMT. It is found that CR cervical cancer cells possessed more EMT characteristics and demonstrated higher migratory abilities and invasiveness. miR-25-3p downregulation was also seen in HeLa-CR and CaSki-CR cells. Of note, ectopic expression of miR-25-3p reversed the EMT phenotype and sensitized CR cells to cisplatin via targeting Sema4C. Furthermore, stable overexpression of miR-25-3p in HeLa-CR cells suppressed tumor growth in mice, downregulated Sema4C and Snail, and upregulated E-cadherin compared with the control group. These results suggest that miR-25-3p is an important regulator of cervical cancer EMT and chemoresistance. Thus, upregulation of miR-25-3p could be a novel approach to treat cervical cancers that are resistant to chemotherapy. © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

Trading Carbon: Can Cookstoves Light the Way (LBNL Science at the Theater)

ScienceCinema

Gadgil, Ashok; Booker, Kayje; Rausch, Adam

2018-06-08

Science at the Theater: Get smart about carbon! Learn how families in Africa, using stoves designed by Berkeley Lab, are at the forefront of global carbon reduction. Ashok Gadgil is the driving force behind the Berkeley-Darfur Cookstove. He is a researcher, inventor, renowned humanitarian, and director of Berkeley Lab's Environmental Energy Technologies Division. Kayje Booker is a Berkeley Lab researcher and UC Berkeley graduate student in ecosystem sciences. She is exploring how carbon markets can serve as catalysts for innovation in technologies for the poor. Adam Rausch is a Berkeley Lab researcher and UC Berkeley graduate student in civil environmental engineering. He helps to design and test stove designs in Ethiopia and elsewhere.

Trading Carbon: Can Cookstoves Light the Way (LBNL Science at the Theater)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gadgil, Ashok; Booker, Kayje; Rausch, Adam

2010-09-20

Science at the Theater: Get smart about carbon! Learn how families in Africa, using stoves designed by Berkeley Lab, are at the forefront of global carbon reduction. Ashok Gadgil is the driving force behind the Berkeley-Darfur Cookstove. He is a researcher, inventor, renowned humanitarian, and director of Berkeley Lab's Environmental Energy Technologies Division. Kayje Booker is a Berkeley Lab researcher and UC Berkeley graduate student in ecosystem sciences. She is exploring how carbon markets can serve as catalysts for innovation in technologies for the poor. Adam Rausch is a Berkeley Lab researcher and UC Berkeley graduate student in civil environmentalmore » engineering. He helps to design and test stove designs in Ethiopia and elsewhere.« less

Dynamic Polymorphic Reconfiguration to Effectively Cloak a Circuit’s Function

DTIC Science & Technology

2011-03-24

86 B . RSA Traces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 2.1...56 22 SEMA of Separate Square and Multiply Trace with key (E B5) - RSA Version B ...56 23 Separate Square and Multiply Trace after signal processing - RSA Version B

Weak Interactions Group

Science.gov Websites

Weak Interactions Group UC Berkeley UC Berkeley Physics Lawrence Berkeley Lab Nuclear Science Division at LBL Physics Division at LBL Phonebook A-Z Index Navigation Home Members Research Projects CUORE Design Concept Berkeley Projects People Publications Contact Links KamLAND Physics Impact Neutrino

Suberoylanilide hydroxamic acid, an inhibitor of histone deacetylase, suppresses vasculogenic mimicry and proliferation of highly aggressive pancreatic cancer PaTu8988 cells

PubMed Central

2014-01-01

Background Pancreatic cancer is one of the most aggressive human malignancies with a extremely low 5-year survival rate. Hence, the search for more effective anti-pancreatic cancer agents is urgent. Methods PaTu8988 pancreatic cancer cells were treated with different concentrations of suberoylanilide hydroxamic acid (SAHA), cell survival, proliferation, migration and vasculogenic mimicry (VM) were analyzed. Associated signaling changes were also analyzed by RT-PCR and Western blots. Results Here, we reported that SAHA, a histone deacetylase inhibitor (HDACi), exerted significant inhibitory efficiency against pancreatic cancer cell survival, proliferation, migration and VM. SAHA dose-dependently inhibited PaTu8988 pancreatic cancer cell growth with the IC-50 of 3.4 ± 0. 7 μM. Meanwhile, SAHA suppressed PaTu8988 cell cycle progression through inducing G2/M arrest, which was associated with cyclin-dependent kinase 1 (CDK-1)/cyclin-B1 degradation and p21/p27 upregulation. Further, SAHA induced both apoptotic and non-apoptotic death of PaTu8988 cells. Significantly, SAHA suppressed PaTu8988 cell in vitro migration and cell-dominant tube formation or VM, which was accompanied by semaphorin-4D (Sema-4D) and integrin-β5 down-regulation. Our evidences showed that Akt activation might be important for Sema-4D expression in PaTu8988 cells, and SAHA-induced Sema-4D down-regulation might be associated with Akt inhibition. Conclusions This study is among the first to report the VM formation in cultured human pancreatic cancer cells. And we provided strong evidence to suggest that SAHA executes significant anti-VM efficiency in the progressive pancreatic cancer cells. Thus, SAHA could be further investigated as a promising anti-pancreatic cancer agent. PMID:24886166

Evaluating the Causal Link Between Malaria Infection and Endemic Burkitt Lymphoma in Northern Uganda: A Mendelian Randomization Study.

PubMed

Legason, Ismail D; Pfeiffer, Ruth M; Udquim, Krizia-Ivana; Bergen, Andrew W; Gouveia, Mateus H; Kirimunda, Samuel; Otim, Isaac; Karlins, Eric; Kerchan, Patrick; Nabalende, Hadijah; Bayanjargal, Ariunaa; Emmanuel, Benjamin; Kagwa, Paul; Talisuna, Ambrose O; Bhatia, Kishor; Yeager, Meredith; Biggar, Robert J; Ayers, Leona W; Reynolds, Steven J; Goedert, James J; Ogwang, Martin D; Fraumeni, Joseph F; Prokunina-Olsson, Ludmila; Mbulaiteye, Sam M

2017-11-01

Plasmodium falciparum (Pf) malaria infection is suspected to cause endemic Burkitt Lymphoma (eBL), but the evidence remains unsettled. An inverse relationship between sickle cell trait (SCT) and eBL, which supports that between malaria and eBL, has been reported before, but in small studies with low power. We investigated this hypothesis in children in a population-based study in northern Uganda using Mendelian Randomization. Malaria-related polymorphisms (SCT, IL10, IL1A, CD36, SEMA3C, and IFNAR1) were genotyped in 202 eBL cases and 624 controls enrolled during 2010-2015. We modeled associations between genotypes and eBL or malaria using logistic regression. SCT was associated with decreased risk of eBL (adjusted odds ratio [OR] 0·37, 95% CI 0·21-0·66; p=0·0003). Decreased risk of eBL was associated with IL10 rs1800896-CT (OR 0·73, 95% CI 0·50-1·07) and -CC genotypes (OR 0·53, 95% CI 0·29-0·95, p trend =0·019); IL1A rs2856838-AG (OR 0·56, 95% CI 0·39-0·81) and -AA genotype (OR 0·50, 95% CI 0·28-1·01, p trend =0·0016); and SEMA3C rs4461841-CT or -CC genotypes (OR 0·57, 95% CI 0·35-0·93, p=0·0193). SCT and IL10 rs1800896, IL1A rs2856838, but not SEMA3C rs4461841, polymorphisms were associated with decreased risk of malaria in the controls. Our results support a causal effect of malaria infection on eBL. Published by Elsevier B.V.

Effectiveness of personalised support for self-management in primary care: a cluster randomised controlled trial

PubMed Central

Eikelenboom, Nathalie; van Lieshout, Jan; Jacobs, Annelies; Verhulst, Frank; Lacroix, Joyca; van Halteren, Aart; Klomp, Maarten; Smeele, Ivo; Wensing, Michel

2016-01-01

Background Self-management support is an important component of the clinical management of many chronic conditions. The validated Self-Management Screening questionnaire (SeMaS) assesses individual characteristics that influence a patient’s ability to self-manage. Aim To assess the effect of providing personalised self-management support in clinical practice on patients’ activation and health-related behaviours. Design and setting A cluster randomised controlled trial was conducted in 15 primary care group practices in the south of the Netherlands. Method After attending a dedicated self-management support training session, practice nurses in the intervention arm discussed the results of SeMaS with the patient at baseline, and tailored the self-management support. Participants completed a 13-item Patient Activation Measure (PAM-13) and validated lifestyle questionnaires at baseline and after 6 months. Data, including individual care plans, referrals to self-management interventions, self-monitoring, and healthcare use, were extracted from patients’ medical records. Multilevel multiple regression was used to assess the effect on outcomes. Results The PAM-13 score did not differ significantly between the control (n = 348) and intervention (n = 296) arms at 6 months. In the intervention arm, 29.4% of the patients performed self-monitoring, versus 15.2% in the control arm (effect size r = 0.9, P = 0.01). In the per protocol analysis (control n = 348; intervention n = 136), the effect of the intervention was significant on the number of individual care plans (effect size r = 1.3, P = 0.04) and on self-monitoring (effect size r = 1.0, P = 0.01). Conclusion This study showed that discussing SeMaS and offering tailored support did not affect patient activation or lifestyle, but did stimulate patients to self-monitor and use individual care plans. PMID:27080318

Sneak Preview of Berkeley Lab's Science at the Theatre on June 6th, 2011

ScienceCinema

Sanii, Babak

2017-12-11

Babak Sanii provides a sneak preview of Berkeley Lab's next Science at the Theater Event: Big Thinking: The Power of Nanoscience. Berkeley Lab scientists reveal how nanoscience will bring us cleaner energy, faster computers, and improved medicine. Berkeley Repertory Theatre on June 6th, 2011.

Sneak Preview of Berkeley Lab's Science at the Theatre on June 6th, 2011

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sanii, Babak

Babak Sanii provides a sneak preview of Berkeley Lab's next Science at the Theater Event: Big Thinking: The Power of Nanoscience. Berkeley Lab scientists reveal how nanoscience will bring us cleaner energy, faster computers, and improved medicine. Berkeley Repertory Theatre on June 6th, 2011.

A Sleeping Beauty forward genetic screen identifies new genes and pathways driving osteosarcoma development and metastasis

PubMed Central

Moriarity, Branden S; Otto, George M; Rahrmann, Eric P; Rathe, Susan K; Wolf, Natalie K; Weg, Madison T; Manlove, Luke A; LaRue, Rebecca S; Temiz, Nuri A; Molyneux, Sam D; Choi, Kwangmin; Holly, Kevin J; Sarver, Aaron L; Scott, Milcah C; Forster, Colleen L; Modiano, Jaime F; Khanna, Chand; Hewitt, Stephen M; Khokha, Rama; Yang, Yi; Gorlick, Richard; Dyer, Michael A; Largaespada, David A

2016-01-01

Osteosarcomas are sarcomas of the bone, derived from osteoblasts or their precursors, with a high propensity to metastasize. Osteosarcoma is associated with massive genomic instability, making it problematic to identify driver genes using human tumors or prototypical mouse models, many of which involve loss of Trp53 function. To identify the genes driving osteosarcoma development and metastasis, we performed a Sleeping Beauty (SB) transposon-based forward genetic screen in mice with and without somatic loss of Trp53. Common insertion site (CIS) analysis of 119 primary tumors and 134 metastatic nodules identified 232 sites associated with osteosarcoma development and 43 sites associated with metastasis, respectively. Analysis of CIS-associated genes identified numerous known and new osteosarcoma-associated genes enriched in the ErbB, PI3K-AKT-mTOR and MAPK signaling pathways. Lastly, we identified several oncogenes involved in axon guidance, including Sema4d and Sema6d, which we functionally validated as oncogenes in human osteosarcoma. PMID:25961939

Genomic Advances to Improve Biomass for Biofuels (LBNL Science at the Theater)

ScienceCinema

Rokhsar, Daniel [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

2018-05-24

Lawrence Berkeley National Lab bioscientist Daniel Rokhsar discusses genomic advances to improve biomass for biofuels. He presented his talk Feb. 11, 2008 in Berkeley, California as part of Berkeley Lab's community lecture series. Rokhsar works with the U.S. Department of Energy's Joint Genome Institute and Berkeley Lab's Genomics Division.

Berkeley Lab Scientist Named MacArthur "Genius" Fellow for Audio

Science.gov Websites

Preservation Research | Berkeley Lab Berkeley Lab A-Z Index Directory Submit Web People Navigation Berkeley Lab Search Submit Web People Close About the Lab Leadership/Organization Calendar News to digitally recover a 128-year-old recording of Alexander Graham Bell's voice, enabling people to

Genomic Advances to Improve Biomass for Biofuels (Genomics and Bioenergy)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rokhsar, Daniel

2008-02-11

Lawrence Berkeley National Lab bioscientist Daniel Rokhsar discusses genomic advances to improve biomass for biofuels. He presented his talk Feb. 11, 2008 in Berkeley, California as part of Berkeley Lab's community lecture series. Rokhsar works with the U.S. Department of Energy's Joint Genome Institute and Berkeley Lab's Genomics Division.

Genomic Advances to Improve Biomass for Biofuels (LBNL Science at the Theater)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rokhsar, Daniel

2008-02-11

Lawrence Berkeley National Lab bioscientist Daniel Rokhsar discusses genomic advances to improve biomass for biofuels. He presented his talk Feb. 11, 2008 in Berkeley, California as part of Berkeley Lab's community lecture series. Rokhsar works with the U.S. Department of Energy's Joint Genome Institute and Berkeley Lab's Genomics Division.

Adsorption of Dissolved Metals in the Berkeley Pit using Thiol-Functionalized Self-Assembled Monolayers on Mesoporous Supports (Thiol-SAMMS)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Betancourt, Amaury P.; Mattigod, Shas V.; Wellman, Dawn M.

2010-03-07

The Berkeley Pit in Butte, Montana, is heavily contaminated with dissolved metals. Adsorption and extraction of these metals can be accomplished through the use of a selective adsorbent. For this research, the adsorbent used was thiol-functionalized Self-Assembled Monolayers on Mesoporous Supports (thiol-SAMMS), which was developed at Pacific Northwest National Laboratory (PNNL). Thiol-SAMMS selectively binds to numerous types of dissolved metals. The objective of this research was to evaluate the loading and kinetics of aluminum, beryllium, copper, and zinc on thiol-SAMMS. For the loading tests, a series of Berkeley Pit water to thiol-SAMMS ratios (mL:g) were tested. These ratios were 1000:1,more » 500:1, 100:1, and 50:1. Berkeley Pit water is acidic (pH {approx} 2.5). This can affect the performance of SAMMS materials. Therefore, the effect of pH was evaluated by conducting parallel series of loading tests wherein the Berkeley Pit water was neutralized before or after addition of thiol-SAMMS, and a series of kinetics tests wherein the Berkeley Pit water was neutralized before addition of thiol-SAMMS for the first test and was not neutralized for the second test. For the kinetics tests, one Berkeley Pit water to thiol-SAMMS ratio was tested, which was 2000:1. The results of the loading and kinetics tests suggest that a significant decrease in dissolved metal concentration at Berkeley Pit could be realized through neutralization of Berkeley Pit water. Thiol-SAMMS technology has a limited application under the highly acidic conditions posed by the Berkeley Pit. However, thiol-SAMMS could provide a secondary remedial technique which would complete the remedial system and remove dissolved metals from the Berkeley Pit to below drinking water standards.« less

The Undergraduate Origins of PhD Economists: The Berkeley Experience

ERIC Educational Resources Information Center

Olney, Martha L.

2015-01-01

The University of California, Berkeley sends more undergraduate students to economics PhD programs than any other public university. While this fact is surely a function of its size, there may be lessons from the Berkeley experience that others could adopt. To investigate why Berkeley generates so many economics PhD students, the author convened…

Microsoft Licenses Berkeley Lab's Home Energy Saver Code for Its Energy

Science.gov Websites

-based tool for calculating energy use in residential buildings. About one million people visit the Home Management Software | Berkeley Lab Berkeley Lab A-Z Index Directory Submit Web People Navigation Berkeley Lab Search Submit Web People Close About the Lab Leadership/Organization Calendar News

What Made Berkeley Great? The Sources of Berkeley's Sustained Academic Excellence. Research & Occasional Paper Series CSHE.3.11

ERIC Educational Resources Information Center

Breslauer, George W.

2011-01-01

University of California (UC) Berkeley's chief academic officer explores the historical sources of Berkeley' academic excellence. He identifies five key factors: (1) wealth from many sources; (2) supportive and skilled governors; (3) leadership from key UC presidents; (4) the pioneering ethos within the State of California; and (5) a process of…

Berkeley Lab's Saul Perlmutter wins Nobel Prize in Physics | Berkeley Lab

Science.gov Websites

astrophysics, dark energy, physics Connect twitter instagram LinkedIn facebook youtube This form needs Berkeley Lab's Saul Perlmutter wins Nobel Prize in Physics News Release Paul Preuss 510-486-6249 * October professor of physics at the University of California at Berkeley, has won the 2011 Nobel Prize in Physics

FY2014 LBNL LDRD Annual Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ho, Darren

2015-06-01

Laboratory (Berkeley Lab or LBNL) is a multi-program national research facility operated by the University of California for the Department of Energy (DOE). As an integral element of DOE’s National Laboratory System, Berkeley Lab supports DOE’s missions in fundamental science, energy resources, and environmental quality. Berkeley Lab programs advance four distinct goals for DOE and the nation. The LDRD program supports Berkeley Lab’s mission in many ways. First, because LDRD funds can be allocated within a relatively short time frame, Berkeley Lab researchers can support the mission of the Department of Energy (DOE) and serve the needs of the nationmore » by quickly responding to forefront scientific problems. Second, LDRD enables Berkeley Lab to attract and retain highly qualified scientists and to support their efforts to carry out worldleading research. In addition, the LDRD program also supports new projects that involve graduate students and postdoctoral fellows, thus contributing to the education mission of Berkeley Lab.« less

Cool Cities, Cool Planet (LBNL Science at the Theater)

ScienceCinema

Rosenfeld, Arthur; Pomerantz, Melvin; Levinson, Ronnen

2018-06-14

Science at the Theater: Berkeley Lab scientists discuss how cool roofs can cool your building, your city ... and our planet. Arthur Rosenfeld, Professor of Physics Emeritus at UC Berkeley, founded the Berkeley Lab Center for Building Science in 1974. He served on the California Energy Commission from 2000 to 2010 and is commonly referred to as California's godfather of energy efficiency. Melvin Pomerantz is a member of the Heat Island Group at Berkeley Lab. Trained as a physicist at UC Berkeley, he specializes in research on making cooler pavements and evaluating their effects. Ronnen Levinson is a staff scientist at Berkeley Lab and the acting leader of its Heat Island Group. He has developed cool roofing and paving materials and helped bring cool roof requirements into building energy efficiency standards.

Untitled Document

Science.gov Websites

, contact: Saul Perlmutter (saul(at)lbl(dot)gov) University of California Berkeley, CA 94720 A. Spadafora (alspadafora(at)lbl(dot)gov) Lawrence Berkeley National Laboratory 1 Cyclotron Rd. Berkeley, CA 94720

Berkeley Lab - Materials Sciences Division

Science.gov Websites

Berkeley Lab Berkeley Lab A-Z Index Phone Book Jobs Search DOE Search MSD Go MSD - Materials Investigators Division Staff Facilities and Centers Staff Jobs Safety Personnel Resources Committees In Case of

ARC-2006-ACD06-0216-008

NASA Image and Video Library

2006-11-29

Stardust sample analysis @ UC Berkeley clean room with Dr Scott Sandford, NASA Ames Astrophysicist - mission samples provided to UC Berkeley for analysis by NASA Berkeley researcher Zack Gainsforth working with sample encased in aerogel

ARC-2006-ACD06-0216-003

NASA Image and Video Library

2006-11-29

Stardust sample analysis @ UC Berkeley clean room with Dr Scott Sandford, NASA Ames Astrophysicist - mission samples provided to UC Berkeley for analysis by NASA Berkeley researchers Zack Gainsforth working with sample encased in aerogel

ARC-2006-ACD06-0216-024

NASA Image and Video Library

2006-11-29

Stardust sample analysis @ UC Berkeley clean room with Dr Scott Sandford, NASA Ames Astrophysicist - mission samples provided to UC Berkeley for analysis by NASA Berkeley researcher Zack Gainsforth working with sample encased in aerogel

ARC-2006-ACD06-0216-025

NASA Image and Video Library

2006-11-29

Stardust sample analysis @ UC Berkeley clean room with Dr Scott Sandford, NASA Ames Astrophysicist - mission samples provided to UC Berkeley for analysis by NASA Berkeley researcher Zack Gainsforth working with sample encased in aerogel

Targeting Transcription Elongation Machinery for Breast Cancer Therapy

DTIC Science & Technology

2016-05-01

Luo CONTRACTING ORGANIZATION: University of California, Berkeley Berkeley, CA 94704 REPORT DATE: May 2016 TYPE OF REPORT: Annual PREPARED FOR...ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBER University of California, Berkeley BERKELEY, CA 94704 9. SPONSORING...molecules. We have employed the CRISPR /Cas9 genome-editing tool to knock out the gene encoding the SEC component AFF4 or knock in a mutant cyclin T1 (AAG

Targeting Transcription Elongation Machinery for Breast Cancer Therapy

DTIC Science & Technology

2016-05-01

Zhou CONTRACTING ORGANIZATION: University of California, Berkeley Berkeley, CA 94704 REPORT DATE: May 2016 TYPE OF REPORT: Annual Report...PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBER AND ADDRESS(ES) University of California, Berkeley Berkeley, CA ...without affecting the Brd4 or PTEFb molecules. We have employed the CRISPR /Cas9 genome-editing tool to knock out the gene encoding the SEC component AFF4

Evaluating the Impact of Open Access at Berkeley: Results from the 2015 Survey of Berkeley Research Impact Initiative (BRII) Funding Recipients

ERIC Educational Resources Information Center

Teplitzky, Samantha; Phillips, Margaret

2016-01-01

The Berkeley Research Impact Initiative (BRII) was one of the first campus-based open access (OA) funds to be established in North America and one of the most active, distributing more than $244,000 to support University of California (UC) Berkeley authors. In April 2015, we conducted a qualitative study of 138 individuals who had received BRII…

Berkeley Lab - Materials Sciences Division

Science.gov Websites

Synthesis Condensed Matter and Materials Physics Scattering and Instrumentation Science Centers Center for Berkeley National Laboratory 1 Cyclotron Road MS 66R0200 Berkeley CA 94720 510-486-4957 A U.S. Department

Nuclear Medicine at Berkeley Lab: From Pioneering Beginnings to Today (LBNL Summer Lecture Series)

ScienceCinema

Budinger, Thomas [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Dept. of Nuclear Medicine & Functional Imaging

2018-01-23

Summer Lecture Series 2006: Thomas Budinger, head of Berkeley Lab's Center for Functional Imaging, discusses Berkeley Lab's rich history pioneering the field of nuclear medicine, from radioisotopes to medical imaging.

ARC-2006-ACD06-0216-006

NASA Image and Video Library

2006-11-29

Stardust sample analysis @ UC Berkeley clean room with Dr Scott Sandford, NASA Ames Astrophysicist - mission samples provided to UC Berkeley for analysis by NASA Berkeley researchers Zack Gainsforth (seated) and Chris Snead working with sample encased in aerogel

ARC-2006-ACD06-0216-009

NASA Image and Video Library

2006-11-29

Stardust sample analysis @ UC Berkeley clean room with Dr Scott Sandford, NASA Ames Astrophysicist - mission samples provided to UC Berkeley for analysis by NASA Berkeley researchers Zack Gainsforth (seated) and Chris Snead working with sample encased in aerogel

Molecular Quantum Mechanics 2010: From Methylene to DNA and Beyond Conference Support

DOE Office of Scientific and Technical Information (OSTI.GOV)

None, None

This grant was $12500 for partial support of an international conference, Molecular Quantum Mechanics 2010, which was held on the campus of the University of California, Berkeley, from 24 to 29 May 2010. The conference involved more than 250 participants. The conference schedule ran from as early as 8:00 AM to as late as 10:30 PM at night, in order to accommodate six historical lectures, 16 plenary lectures, 42 invited talks and two very strong poster sessions containing 143 contributed posters. Since 1989, the Molecular Quantum Mechanics (MQM) series of international conferences has show- cased the frontiers of research inmore » quantum chemistry with a strong focus on basic theory and algorithms, as well as highlights of topical applications. Both were strongly in evidence at MQM 2010. At the same time as embracing the future, the MQM conferences also honour the lifetime contributions of some of the most prominent scientists in the field of theoretical and computational quantum chemistry. MQM 2010 recognised the work of Prof. Henry F. ‘Fritz’ Schaefer of the Center for Computational Chemistry at the University of Georgia, who was previously on the faculty at Berkeley The travel of invited speakers was partially covered by sponsorships from Dell Computer, Hewlett-Packard, Journal of Chemical Theory and Computation, Virginia Tech College of Science, Molecular Physics, Q-Chem Inc and the American Institute of Physics. By contrast, the conference grant from the Department of Energy was used to provide fellowships and scholarships to enable graduate students and postdoctoral fellows to attend the meeting, and thereby broaden the participation of young scientists at a meeting where in the past most of the attendees have been more senior faculty researchers. We believe that we were very successful in this regard: 118 students and postdocs attended out of the total of 256 participants. In detail, the DOE sponsorship money was partially used for dormitory scholarships that covered the cost of shared accommodation for students and postdocs at Berkeley dormitories. This covered the $200-$305 cost of a shared room for the 5-day duration of the conference. The only condition of these scholarships was that the awardee must present a poster at the meeting. Approximately $7565 was spent for these dormitory scholarships. The remaining expenditures of $4800 was used for 12 merit scholarships which were awarded to students whose poster presentations were judged the best at the conference. This amount covered a significant part of their travel and registration fees.« less

Anti-SEMA4D Monoclonal Antibody VX15/2503 With Nivolumab or Ipilimumab in Treating Patients With Stage III or IV Melanoma

ClinicalTrials.gov

2018-06-15

Metastatic Melanoma; Stage III Cutaneous Melanoma AJCC v7; Stage IIIA Cutaneous Melanoma AJCC v7; Stage IIIB Cutaneous Melanoma AJCC v7; Stage IIIC Cutaneous Melanoma AJCC v7; Stage IV Cutaneous Melanoma AJCC v6 and v7

The laminar organization of the Drosophila ellipsoid body is semaphorin-dependent and prevents the formation of ectopic synaptic connections

PubMed Central

Xie, Xiaojun; Tabuchi, Masashi; Brown, Matthew P; Mitchell, Sarah P; Wu, Mark N; Kolodkin, Alex L

2017-01-01

The ellipsoid body (EB) in the Drosophila brain is a central complex (CX) substructure that harbors circumferentially laminated ring (R) neuron axons and mediates multifaceted sensory integration and motor coordination functions. However, what regulates R axon lamination and how lamination affects R neuron function remain unknown. We show here that the EB is sequentially innervated by small-field and large-field neurons and that early developing EB neurons play an important regulatory role in EB laminae formation. The transmembrane proteins semaphorin-1a (Sema-1a) and plexin A function together to regulate R axon lamination. R neurons recruit both GABA and GABA-A receptors to their axon terminals in the EB, and optogenetic stimulation coupled with electrophysiological recordings show that Sema-1a-dependent R axon lamination is required for preventing the spread of synaptic inhibition between adjacent EB lamina. These results provide direct evidence that EB lamination is critical for local pre-synaptic inhibitory circuit organization. DOI: http://dx.doi.org/10.7554/eLife.25328.001 PMID:28632130

DOE Office of Scientific and Technical Information (OSTI.GOV)

Russell, Sabin; Schlegel, David

Lawrence Berkeley National Laboratory physicist and dark energy hunter David Schlegel chats with Sabin Russell, former San Francisco Chronicle reporter turned Berkeley Lab science writer, June 22, 2011. Their conversation is the first installment of "Sit Down With Sabin," a weekly conversation hosted by Russell. Over the course of five conversations with Berkeley Lab staff this summer, Russell will explore the ups and downs of innovative science — all without the aid of PowerPoint slides. Brought to you by Berkeley Lab Public Affairs.

Frequent epigenetic inactivation of chromosome 3p candidate tumor suppressor genes in gallbladder carcinoma.

PubMed

Riquelme, Erick; Tang, Moying; Baez, Sergio; Diaz, Alfonso; Pruyas, Martha; Wistuba, Ignacio I; Corvalan, Alejandro

2007-05-18

Gallbladder carcinoma (GBC) is a highly malignant neoplasm that represents the leading cause of death for cancer in Chilean females. There is limited information about the molecular abnormalities involved in its pathogenesis. We have identified a number of molecular changes in GBC, including frequent allelic losses at chromosome 3p regions. Four distinct 3p sites (3p12, 3p14.2, 3p21.3 and 3p22-24) with frequent and early allelic losses in the sequential pathogenesis of this neoplasm have been detected. We investigated epigenetic and genetic abnormalities in GBC affecting 6 candidate tumor suppressor genes (TSG) located in chromosome 3p, including DUTT1 (3p12), FHIT (3p14.2), BLU, RASSF1A, SEMA3B and hMLH1 (3p21.3). DNA extracted from frozen tissue obtained from 50 surgical resected GBCs was examined for gene promoter methylation using MSP (methylation-specific PCR) technique after bisulfite treatment in all 6 genes. In addition, we performed PCR-based mutation examination using SSCP in FHIT and RASSF1A genes and loss of heterozygosity (LOH) analysis using microdissected tissue in a subset of tumors for the 3p21.3 region with 8 microsatellite markers. A very high frequency of GBC methylation was detected in SEMA3B (46/50, 92%) and FHIT (33/50, 66%), intermediate incidences in BLU (13/50, 26%) and DUTT1 (11/50, 22%) and very low frequencies in RASSF1A (4/50, 8%) and hMLH1 (2/50, 4%). Allelic loss at 3p21.3 was found in nearly half of the GBCs examined. We conclude that epigenetic inactivation by abnormal promoter methylation is a frequent event in chromosome 3p candidate TSGs in GBC pathogenesis, especially affecting genes SEMA3B (3p21.3) and FHIT (3p14.2).

40 CFR 81.341 - South Carolina.

Code of Federal Regulations, 2014 CFR

2014-07-01

... County X Anderson County X Bamberg County X Barnwell County X Beaufort County X Berkeley County X Calhoun... County X Berkeley County X Calhoun County X Charleston County X Cherokee County X Chester County X... Beaufort County Berkeley County Calhoun County Charleston County Cherokee County Chester County...

Berkeley Screen: a set of 96 solutions for general macromolecular crystallization

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pereira, Jose H.; McAndrew, Ryan P.; Tomaleri, Giovani P.

Using statistical analysis of the Biological Macromolecular Crystallization Database, combined with previous knowledge about crystallization reagents, a crystallization screen called the Berkeley Screen has been created. Correlating crystallization conditions and high-resolution protein structures, it is possible to better understand the influence that a particular solution has on protein crystal formation. Ions and small molecules such as buffers and precipitants used in crystallization experiments were identified in electron density maps, highlighting the role of these chemicals in protein crystal packing. The Berkeley Screen has been extensively used to crystallize target proteins from the Joint BioEnergy Institute and the Collaborative Crystallography programmore » at the Berkeley Center for Structural Biology, contributing to several Protein Data Bank entries and related publications. The Berkeley Screen provides the crystallographic community with an efficient set of solutions for general macromolecular crystallization trials, offering a valuable alternative to the existing commercially available screens. The Berkeley Screen provides an efficient set of solutions for general macromolecular crystallization trials.« less

Berkeley Screen: a set of 96 solutions for general macromolecular crystallization

DOE PAGES

Pereira, Jose H.; McAndrew, Ryan P.; Tomaleri, Giovani P.; ...

2017-09-05

Using statistical analysis of the Biological Macromolecular Crystallization Database, combined with previous knowledge about crystallization reagents, a crystallization screen called the Berkeley Screen has been created. Correlating crystallization conditions and high-resolution protein structures, it is possible to better understand the influence that a particular solution has on protein crystal formation. Ions and small molecules such as buffers and precipitants used in crystallization experiments were identified in electron density maps, highlighting the role of these chemicals in protein crystal packing. The Berkeley Screen has been extensively used to crystallize target proteins from the Joint BioEnergy Institute and the Collaborative Crystallography programmore » at the Berkeley Center for Structural Biology, contributing to several Protein Data Bank entries and related publications. The Berkeley Screen provides the crystallographic community with an efficient set of solutions for general macromolecular crystallization trials, offering a valuable alternative to the existing commercially available screens. The Berkeley Screen provides an efficient set of solutions for general macromolecular crystallization trials.« less

Web Support

Science.gov Websites

Berkeley Lab Lawrence Berkeley National Laboratory A-Z Index Directory Submit Web People Navigation Berkeley Lab Search Submit Web People Close About the Lab Leadership/Organization Calendar News Center our response, please check the specific website or page in question for the name of the appropriate

78 FR 29022 - Safety Zone; Fourth of July Fireworks, Berkeley Marina, Berkeley, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-17

... Marina Fourth of July Fireworks display in the Captain of the Port, San Francisco area of responsibility... Guard will enforce a 1,000 foot safety zone around the Berkeley Pier in approximate position 37[deg]51... radius 1,000 [[Page 29023

Berkeley Lab - Materials Sciences Division

Science.gov Websites

Synthesis Condensed Matter and Materials Physics Scattering and Instrumentation Science Centers Center for Berkeley Lab Berkeley Lab A-Z Index Phone Book Jobs Search DOE Search MSD Go MSD - Materials Sciences Division About Organization Contact Research Core Programs Materials Discovery, Design and

Molecular Foundry Workshop draws overflow crowd to BerkeleyLab

DOE Office of Scientific and Technical Information (OSTI.GOV)

Robinson, Art

2002-11-27

Nanoscale science and technology is now one of the top research priorities in the United States. With this background, it is no surprise that an overflow crowd or more than 350 registrants filled two auditoriums to hear about and contribute ideas for the new Molecular Foundry during a two-day workshop at the Lawrence Berkeley National Laboratory (Berkeley Lab). Scheduled to open for business at Berkeley Labin early 2006, the Molecular Foundry is one of three Nanoscale Science Research Centers (NSRCs) put forward for funding by the DOE's Office of Basic Energy Sciences (BES).

Excellence in Research: Creative Organizational Responses at Berkeley, Harvard, MIT, and Stanford. ASHE 1985 Annual Meeting Paper.

ERIC Educational Resources Information Center

Gardiner, John J.

Research environments of four leading universities were studied: University of California at Berkeley (UC-Berkeley), Harvard University, Massachusetts Institute of Technology (MIT), and Stanford University. Attention was directed to organizational responses for encouraging collaboration in research at these leading universities, as well as to…

Berkeley Lab - Lawrence Berkeley National Laboratory

Science.gov Websites

nanoparticles that could make solar panels more efficient by converting light usually missed by solar cells into of Methane's Increasing Greenhouse Effect A Berkeley Lab research team tracked a rise in the warming effect of methane - one of the most important greenhouse gases for the Earth's atmosphere - over a 10

A-Z Link

Science.gov Websites

Index (this page) 2. Use search.lbl.gov powered by Google. 3. Use DS The Directory of both People and Berkeley Lab Lawrence Berkeley National Laboratory A-Z Index Directory Submit Web People Navigation Berkeley Lab Search Submit Web People Close About the Lab Leadership/Organization Calendar News Center

Berkeley Lab - Materials Sciences Division

Science.gov Websites

Synthesis Condensed Matter and Materials Physics Scattering and Instrumentation Science Centers Center for materials and phenomena at multiple time and length scales. Through our core programs and research centers Berkeley Lab Berkeley Lab A-Z Index Phone Book Jobs Search DOE Search MSD Go MSD - Materials

ARC-2006-ACD06-0216-010

NASA Image and Video Library

2006-11-29

Stardust sample analysis @ UC Berkeley clean room with Dr Scott Sandford, NASA Ames Astrophysicist - mission samples provided to UC Berkeley for analysis by NASA Berkeley researchers Zack Gainsforth (seated) and Chris Snead working with sample encased in aerogel Note: Eric Land of NASA/AMES video crew in lower left corner providing sound support for event

City of Berkeley, California Municipal Tree Resource Analysis

Treesearch

S.E. Maco; E.G. McPherson; J.R. Simpson; P.J. Peper; Q. Xiao

2005-01-01

Vibrant, renowned for its livability and cultural wealth, the city of Berkeley maintains trees as an integral component of the urban infrastructure. Research indicates that healthy trees can mitigate impacts associated with the built environment by reducing stormwater runoff, energy consumption, and air pollutants. Put simply, trees improve urban life, making Berkeley...

PUB-3000 | BERKELEY LAB HEALTH AND SAFETY MANUAL

Science.gov Websites

ES&H MANUAL (PUB-3000) Berkeley Lab Table of Contents Guide to Using the ES&H Manual Responsible Authors Log of ES&H Manual Changes Requesting a Change to the ES&H Manual Search the ES &H Manual Questions & Comments Lawrence Berkeley National Laboratory University of California

Changes in prices, sales, consumer spending, and beverage consumption one year after a tax on sugar-sweetened beverages in Berkeley, California, US: A before-and-after study.

PubMed

Silver, Lynn D; Ng, Shu Wen; Ryan-Ibarra, Suzanne; Taillie, Lindsey Smith; Induni, Marta; Miles, Donna R; Poti, Jennifer M; Popkin, Barry M

2017-04-01

Taxes on sugar-sweetened beverages (SSBs) meant to improve health and raise revenue are being adopted, yet evaluation is scarce. This study examines the association of the first penny per ounce SSB excise tax in the United States, in Berkeley, California, with beverage prices, sales, store revenue/consumer spending, and usual beverage intake. Methods included comparison of pre-taxation (before 1 January 2015) and first-year post-taxation (1 March 2015-29 February 2016) measures of (1) beverage prices at 26 Berkeley stores; (2) point-of-sale scanner data on 15.5 million checkouts for beverage prices, sales, and store revenue for two supermarket chains covering three Berkeley and six control non-Berkeley large supermarkets in adjacent cities; and (3) a representative telephone survey (17.4% cooperation rate) of 957 adult Berkeley residents. Key hypotheses were that (1) the tax would be passed through to the prices of taxed beverages among the chain stores in which Berkeley implemented the tax in 2015; (2) sales of taxed beverages would decline, and sales of untaxed beverages would rise, in Berkeley stores more than in comparison non-Berkeley stores; (3) consumer spending per transaction (checkout episode) would not increase in Berkeley stores; and (4) self-reported consumption of taxed beverages would decline. Main outcomes and measures included changes in inflation-adjusted prices (cents/ounce), beverage sales (ounces), consumers' spending measured as store revenue (inflation-adjusted dollars per transaction) in two large chains, and usual beverage intake (grams/day and kilocalories/day). Tax pass-through (changes in the price after imposition of the tax) for SSBs varied in degree and timing by store type and beverage type. Pass-through was complete in large chain supermarkets (+1.07¢/oz, p = 0.001) and small chain supermarkets and chain gas stations (1.31¢/oz, p = 0.004), partial in pharmacies (+0.45¢/oz, p = 0.03), and negative in independent corner stores and independent gas stations (-0.64¢/oz, p = 0.004). Sales-unweighted mean price change from scanner data was +0.67¢/oz (p = 0.00) (sales-weighted, +0.65¢/oz, p = 0.003), with +1.09¢/oz (p < 0.001) for sodas and energy drinks, but a lower change in other categories. Post-tax year 1 scanner data SSB sales (ounces/transaction) in Berkeley stores declined 9.6% (p < 0.001) compared to estimates if the tax were not in place, but rose 6.9% (p < 0.001) for non-Berkeley stores. Sales of untaxed beverages in Berkeley stores rose by 3.5% versus 0.5% (both p < 0.001) for non-Berkeley stores. Overall beverage sales also rose across stores. In Berkeley, sales of water rose by 15.6% (p < 0.001) (exceeding the decline in SSB sales in ounces); untaxed fruit, vegetable, and tea drinks, by 4.37% (p < 0.001); and plain milk, by 0.63% (p = 0.01). Scanner data mean store revenue/consumer spending (dollars per transaction) fell 18¢ less in Berkeley (-$0.36, p < 0.001) than in comparison stores (-$0.54, p < 0.001). Baseline and post-tax Berkeley SSB sales and usual dietary intake were markedly low compared to national levels (at baseline, National Health and Nutrition Examination Survey SSB intake nationally was 131 kcal/d and in Berkeley was 45 kcal/d). Reductions in self-reported mean daily SSB intake in grams (-19.8%, p = 0.49) and in mean per capita SSB caloric intake (-13.3%, p = 0.56) from baseline to post-tax were not statistically significant. Limitations of the study include inability to establish causal links due to observational design, and the absence of health outcomes. Analysis of consumption was limited by the small effect size in relation to high standard error and Berkeley's low baseline consumption. One year following implementation of the nation's first large SSB tax, prices of SSBs increased in many, but not all, settings, SSB sales declined, and sales of untaxed beverages (especially water) and overall study beverages rose in Berkeley; overall consumer spending per transaction in the stores studied did not rise. Price increases for SSBs in two distinct data sources, their timing, and the patterns of change in taxed and untaxed beverage sales suggest that the observed changes may be attributable to the tax. Post-tax self-reported SSB intake did not change significantly compared to baseline. Significant declines in SSB sales, even in this relatively affluent community, accompanied by revenue used for prevention suggest promise for this policy. Evaluation of taxation in jurisdictions with more typical SSB consumption, with controls, is needed to assess broader dietary and potential health impacts.

Robotic technology evolution and transfer

NASA Technical Reports Server (NTRS)

Marzwell, Neville I.

1992-01-01

A report concerning technology transfer in the area of robotics is presented in vugraph form. The following topics are discussed: definition of technology innovation and tech-transfer; concepts relevant for understanding tech-transfer; models advanced to portray tech-transfer process; factors identified as promoting tech-transfer; factors identified as impeding tech-transfer; what important roles do individuals fulfill in tech-transfer; federal infrastructure for promoting tech-transfer; federal infrastructure for promoting tech-transfer; robotic technology evolution; robotic technology transferred; and recommendations for successful robotics tech-transfer.

Mendelevium: The Way It Was

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

A reel of black & white film shot nearly 60 years ago has surfaced at Berkeley Lab, depicting the discovery of Mendelevium - or Element 101 - as reenacted by some of the legendary scientists who did the actual work at that time. Since the 1940s, Berkeley Lab scientists were locked in a race to synthesize new elements, and more often than not, they came out winners. Sixteen elements, most of them in the actinide series at the bottom of the periodic table, were discovered and synthesized by its researchers. Retired Berkeley Lab physicist Claude Lyneis found the reel inmore » a box of dusty and deteriorating films slated for disposal. Using digital editing skills he acquired to make videos of his son's lacrosse team, Lyneis has produced and narrated an excerpt of this nearly-lost footage. It is an entertaining and informative look at the pioneering physics performed at UC Berkeley and Lawrence Berkeley National Laboratory's hillside campus.« less

Laboratory Directed Research and Development Program FY 2006

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hansen

2007-03-08

The Ernest Orlando Lawrence Berkeley National Laboratory (Berkeley Lab or LBNL) is a multi-program national research facility operated by the University of California for the Department of Energy (DOE). As an integral element of DOE's National Laboratory System, Berkeley Lab supports DOE's missions in fundamental science, energy resources, and environmental quality. Berkeley Lab programs advance four distinct goals for DOE and the nation: (1) To perform leading multidisciplinary research in the computing sciences, physical sciences, energy sciences, biosciences, and general sciences in a manner that ensures employee and public safety and protection of the environment. (2) To develop and operatemore » unique national experimental facilities for qualified investigators. (3) To educate and train future generations of scientists and engineers to promote national science and education goals. (4) To transfer knowledge and technological innovations and to foster productive relationships among Berkeley Lab's research programs, universities, and industry in order to promote national economic competitiveness.« less

Mendelevium: The Way It Was

ScienceCinema

None

2018-05-30

A reel of black & white film shot nearly 60 years ago has surfaced at Berkeley Lab, depicting the discovery of Mendelevium - or Element 101 - as reenacted by some of the legendary scientists who did the actual work at that time. Since the 1940s, Berkeley Lab scientists were locked in a race to synthesize new elements, and more often than not, they came out winners. Sixteen elements, most of them in the actinide series at the bottom of the periodic table, were discovered and synthesized by its researchers. Retired Berkeley Lab physicist Claude Lyneis found the reel in a box of dusty and deteriorating films slated for disposal. Using digital editing skills he acquired to make videos of his son's lacrosse team, Lyneis has produced and narrated an excerpt of this nearly-lost footage. It is an entertaining and informative look at the pioneering physics performed at UC Berkeley and Lawrence Berkeley National Laboratory's hillside campus.

Occupational Field 66 (Avionics) Less MOS’s 6682, 6683 and 6689 Task Analysis.

DTIC Science & Technology

1979-04-01

EQUIPMENT ( SACE ) TECH 011 ACFT CRYPTOGRAPHIC SYS TECI-, IMA 018 ACFT INERTIAL NAVIGATION SYSTEM (INS) SACE TECH 019 ACFT SEARCH/TRACK (SIT) SACE TECH...020 SACE SYS TECH 021 ACFT DECEPTIVE ELECTRONIC COUNTERMEASURES IDECM) TECH 022 ELECTRONIC COUNTERMEASURES (ECM) MODULE REPAIR TECH 023 ACFT ECM TECH

Characterizing Crowd Participation and Productivity of Foldit Through Web Scraping

DTIC Science & Technology

2016-03-01

Berkeley Open Infrastructure for Network Computing CDF Cumulative Distribution Function CPU Central Processing Unit CSSG Crowdsourced Serious Game...computers at once can create a similar capacity. According to Anderson [6], principal investigator for the Berkeley Open Infrastructure for Network...extraterrestrial life. From this project, a software-based distributed computing platform called the Berkeley Open Infrastructure for Network Computing

Participatory Classification in a System for Assessing Multimodal Transportation Patterns

DTIC Science & Technology

2015-02-17

Culler Electrical Engineering and Computer Sciences University of California at Berkeley Technical Report No. UCB/EECS-2015-8 http...California at Berkeley,Electrical Engineering and Computer Sciences,Berkeley,CA,94720 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING...confirmation screen This section sketches the characteristics of the data that was collected, computes the accuracy of the auto- mated inference algorithm

Site Environmental Report for 2004. Volume 1, Environment, Health, and Safety Division

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2005-09-30

Each year, Ernest Orlando Lawrence Berkeley National Laboratory prepares an integrated report on its environmental programs to satisfy the requirements of United States Department of Energy Order 231.1A, Environment, Safety, and Health Reporting.1 The Site Environmental Report for 2004 summarizes Berkeley Lab’s environmental management performance, presents environmental monitoring results, and describes significant programs for calendar year 2004. (Throughout this report, Ernest Orlando Lawrence Berkeley National Laboratory is referred to as “Berkeley Lab,” “the Laboratory,” “Lawrence Berkeley National Laboratory,” and “LBNL.”) The report is separated into two volumes. Volume I contains an overview of the Laboratory, the status of environmental programs,more » and summarized results from surveillance and monitoring activities. Volume II contains individual data results from these activities. This year, the Site Environmental Report was distributed by releasing it on the Web from the Berkeley Lab Environmental Services Group (ESG) home page, which is located at http://www.lbl.gov/ehs/esg/. Many of the documents cited in this report also are accessible from the ESG Web page. CD and printed copies of this Site Environmental Report are available upon request.« less

Star Formation near Berkeley 59: Embedded Protostars

NASA Astrophysics Data System (ADS)

Rosvick, J. M.; Majaess, D.

2013-12-01

A group of suspected protostars in a dark cloud northwest of the young (~2 Myr) cluster Berkeley 59 and two sources in a pillar south of the cluster have been studied in order to determine their evolutionary stages and ascertain whether their formation was triggered by Berkeley 59. Narrowband near-infrared observations from the Observatoire du Mont Mégantic, 12CO (J = 3-2) and SCUBA-2 (450 and 850 μm) observations from the JCMT, 2MASS, and WISE images, and data extracted from the IPHAS survey catalog were used. Of 12 sources studied, two are Class I objects, while three others are flat/Class II, one of which is a T Tauri candidate. A weak CO outflow and two potential starless cores are present in the cloud, while the pillar possesses substructure at different velocities, with no outflows present. The CO spectra of both regions show peaks in the range v LSR = -15 to -17 km s-1, which agrees with the velocity adopted for Berkeley 59 (-15.7 km s-1), while spectral energy distribution models yield an average interstellar extinction AV and distance of 15 ± 2 mag and 830 ± 120 pc, respectively, for the cloud, and 6.9 mag and 912 pc for the pillar, indicating that the regions are in the same vicinity as Berkeley 59. The formation of the pillar source appears to have been triggered by Berkeley 59. It is unclear whether Berkeley 59 triggered the association's formation.

Changes in prices, sales, consumer spending, and beverage consumption one year after a tax on sugar-sweetened beverages in Berkeley, California, US: A before-and-after study

PubMed Central

Ryan-Ibarra, Suzanne; Taillie, Lindsey Smith; Induni, Marta

2017-01-01

Background Taxes on sugar-sweetened beverages (SSBs) meant to improve health and raise revenue are being adopted, yet evaluation is scarce. This study examines the association of the first penny per ounce SSB excise tax in the United States, in Berkeley, California, with beverage prices, sales, store revenue/consumer spending, and usual beverage intake. Methods and findings Methods included comparison of pre-taxation (before 1 January 2015) and first-year post-taxation (1 March 2015–29 February 2016) measures of (1) beverage prices at 26 Berkeley stores; (2) point-of-sale scanner data on 15.5 million checkouts for beverage prices, sales, and store revenue for two supermarket chains covering three Berkeley and six control non-Berkeley large supermarkets in adjacent cities; and (3) a representative telephone survey (17.4% cooperation rate) of 957 adult Berkeley residents. Key hypotheses were that (1) the tax would be passed through to the prices of taxed beverages among the chain stores in which Berkeley implemented the tax in 2015; (2) sales of taxed beverages would decline, and sales of untaxed beverages would rise, in Berkeley stores more than in comparison non-Berkeley stores; (3) consumer spending per transaction (checkout episode) would not increase in Berkeley stores; and (4) self-reported consumption of taxed beverages would decline. Main outcomes and measures included changes in inflation-adjusted prices (cents/ounce), beverage sales (ounces), consumers’ spending measured as store revenue (inflation-adjusted dollars per transaction) in two large chains, and usual beverage intake (grams/day and kilocalories/day). Tax pass-through (changes in the price after imposition of the tax) for SSBs varied in degree and timing by store type and beverage type. Pass-through was complete in large chain supermarkets (+1.07¢/oz, p = 0.001) and small chain supermarkets and chain gas stations (1.31¢/oz, p = 0.004), partial in pharmacies (+0.45¢/oz, p = 0.03), and negative in independent corner stores and independent gas stations (−0.64¢/oz, p = 0.004). Sales-unweighted mean price change from scanner data was +0.67¢/oz (p = 0.00) (sales-weighted, +0.65¢/oz, p = 0.003), with +1.09¢/oz (p < 0.001) for sodas and energy drinks, but a lower change in other categories. Post-tax year 1 scanner data SSB sales (ounces/transaction) in Berkeley stores declined 9.6% (p < 0.001) compared to estimates if the tax were not in place, but rose 6.9% (p < 0.001) for non-Berkeley stores. Sales of untaxed beverages in Berkeley stores rose by 3.5% versus 0.5% (both p < 0.001) for non-Berkeley stores. Overall beverage sales also rose across stores. In Berkeley, sales of water rose by 15.6% (p < 0.001) (exceeding the decline in SSB sales in ounces); untaxed fruit, vegetable, and tea drinks, by 4.37% (p < 0.001); and plain milk, by 0.63% (p = 0.01). Scanner data mean store revenue/consumer spending (dollars per transaction) fell 18¢ less in Berkeley (−$0.36, p < 0.001) than in comparison stores (−$0.54, p < 0.001). Baseline and post-tax Berkeley SSB sales and usual dietary intake were markedly low compared to national levels (at baseline, National Health and Nutrition Examination Survey SSB intake nationally was 131 kcal/d and in Berkeley was 45 kcal/d). Reductions in self-reported mean daily SSB intake in grams (−19.8%, p = 0.49) and in mean per capita SSB caloric intake (−13.3%, p = 0.56) from baseline to post-tax were not statistically significant. Limitations of the study include inability to establish causal links due to observational design, and the absence of health outcomes. Analysis of consumption was limited by the small effect size in relation to high standard error and Berkeley’s low baseline consumption. Conclusions One year following implementation of the nation’s first large SSB tax, prices of SSBs increased in many, but not all, settings, SSB sales declined, and sales of untaxed beverages (especially water) and overall study beverages rose in Berkeley; overall consumer spending per transaction in the stores studied did not rise. Price increases for SSBs in two distinct data sources, their timing, and the patterns of change in taxed and untaxed beverage sales suggest that the observed changes may be attributable to the tax. Post-tax self-reported SSB intake did not change significantly compared to baseline. Significant declines in SSB sales, even in this relatively affluent community, accompanied by revenue used for prevention suggest promise for this policy. Evaluation of taxation in jurisdictions with more typical SSB consumption, with controls, is needed to assess broader dietary and potential health impacts. PMID:28419108

The Berkeley Out-of-Order Machine (BOOM): An Industry-Competitive, Synthesizable, Parameterized RISC-V Processor

DTIC Science & Technology

2015-06-13

The Berkeley Out-of-Order Machine (BOOM): An Industry- Competitive, Synthesizable, Parameterized RISC-V Processor Christopher Celio David A...Synthesizable, Parameterized RISC-V Processor Christopher Celio, David Patterson, and Krste Asanović University of California, Berkeley, California 94720...Order Machine BOOM is a synthesizable, parameterized, superscalar out- of-order RISC-V core designed to serve as the prototypical baseline processor

Berkeley Lab - Materials Sciences Division

Science.gov Websites

Berkeley Lab Berkeley Lab A-Z Index Phone Book Jobs Search DOE Search MSD Go MSD - Materials Investigators Ager, Joel W » Alivisatos, A Paul » Altman, Ehud » Analytis, James » Anderson, Christopher  , Naomi » Gullikson, Eric M » Harris, Stephen J » Hasan, M. Zahid » Hellman, Frances » Helms, Brett A

A Profile of Minority Graduate Students at the University of California, Berkeley: Recruitment, Selection, Fields of Study and Financial Support.

ERIC Educational Resources Information Center

Collins, O. R.

This paper presents a profile of minority graduate students at the University of California, Berkeley. Following a brief overview of Berkeley's Graduate Minority Program (GMP), data is presented concerning the number of GMP students supported; available funds and average grants for students from 1968-69 to 1973-74; distribution of GMP students…

DOE Office of Scientific and Technical Information (OSTI.GOV)

Walker, Iain; Regnier, Cindy

Science at the Theater: Berkeley Lab scientists reveal the latest research on how to reduce your carbon footprint at home, work, and when you shop. Learn how even small choices can have a big impact. Iain Walker's research focuses on optimizing the energy use and comfort of buildings. He's a staff scientist in the Energy Performance of Buildings Group, which is part of Berkeley Lab's Environmen...tal Energy Technologies Division. He's also executive editor of Home Energy Magazine. Cindy Regnier is a Project Manager in the Environmental Energy Technologies Division at Berkeley Lab. She has over 13 years of mechanical engineeringmore » design experience, with a focus on low-energy buildings. Her projects have included several LEED Platinum buildings and the design of a 200,000 sf carbon neutral, net-zero energy science museum in San Francisco. Eric Masanet is Acting Deputy Leader of the International Energy Studies Group at Berkeley Lab. His research focuses on life-cycle assessments and energy efficiency analysis. He holds a joint research appointment in the Institute of Transportation Studies at UC Berkeley.« less

Site Environmental Report for 2006. Volume I, Environment, Health, and Safety Division

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2007-09-30

Each year, Ernest Orlando Lawrence Berkeley National Laboratory prepares an integrated report on its environmental programs to satisfy the requirements of United States Department of Energy Order 231.1A, Environment, Safety, and Health Reporting.1 The Site Environmental Report for 2006 summarizes Berkeley Lab’s environmental management performance, presents environmental monitoring results, and describes significant programs for calendar year 2006. (Throughout this report, Ernest Orlando Lawrence Berkeley National Laboratory is referred to as “Berkeley Lab,” “the Laboratory,” “Lawrence Berkeley National Laboratory,” and “LBNL.”) The report is separated into two volumes. Volume I is organized into an executive summary followed by six chapters thatmore » contain an overview of the Laboratory, a discussion of the Laboratory’s environmental management system, the status of environmental programs, and summarized results from surveillance and monitoring activities. Volume II contains individual data results from surveillance and monitoring activities.« less

Laboratory directed research and development program FY 1999

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hansen, Todd; Levy, Karin

2000-03-08

The Ernest Orlando Lawrence Berkeley National Laboratory (Berkeley Lab or LBNL) is a multi-program national research facility operated by the University of California for the Department of Energy (DOE). As an integral element of DOE's National Laboratory System, Berkeley Lab supports DOE's missions in fundamental science, energy resources, and environmental quality. Berkeley Lab programs advance four distinct goals for DOE and the nation: (1) To perform leading multidisciplinary research in the computing sciences, physical sciences, energy sciences, biosciences, and general sciences in a manner that ensures employee and public safety and protection of the environment. (2) To develop and operatemore » unique national experimental facilities for qualified investigators. (3) To educate and train future generations of scientists and engineers to promote national science and education goals. (4) To transfer knowledge and technological innovations and to foster productive relationships among Berkeley Lab's research programs, universities, and industry in order to promote national economic competitiveness. This is the annual report on Laboratory Directed Research and Development (LDRD) program for FY99.« less

Impact of the Berkeley Excise Tax on Sugar-Sweetened Beverage Consumption

PubMed Central

Falbe, Jennifer; Thompson, Hannah R.; Becker, Christina M.; Rojas, Nadia; McCulloch, Charles E.

2016-01-01

Objectives. To evaluate the impact of the excise tax on sugar-sweetened beverage (SSB) consumption in Berkeley, California, which became the first US jurisdiction to implement such a tax ($0.01/oz) in March 2015. Methods. We used a repeated cross-sectional design to examine changes in pre- to posttax beverage consumption in low-income neighborhoods in Berkeley versus in the comparison cities of Oakland and San Francisco, California. A beverage frequency questionnaire was interviewer administered to 990 participants before the tax and 1689 after the tax (approximately 8 months after the vote and 4 months after implementation) to examine relative changes in consumption. Results. Consumption of SSBs decreased 21% in Berkeley and increased 4% in comparison cities (P = .046). Water consumption increased more in Berkeley (+63%) than in comparison cities (+19%; P < .01). Conclusions. Berkeley’s excise tax reduced SSB consumption in low-income neighborhoods. Evaluating SSB taxes in other cities will improve understanding of their public health benefit and their generalizability. PMID:27552267

From Relational Interfaces to Assume-Guarantee Contracts

DTIC Science & Technology

2014-03-18

Electrical Engineering and Computer Sciences University of California at Berkeley Technical Report No. UCB/EECS-2014-21 http://www.eecs.berkeley.edu/Pubs...5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) University of California at Berkeley, Electrical Engineering...design,” in EMSOFT’01. Springer, LNCS 2211, 2001. [2] A. Sangiovanni-Vincentelli et al., “Taming Dr. Frankenstein : Contract-Based Design for Cyber

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jeff Neaton

Jan. 22, 2010: Host Alice Egan of Berkeley Lab's Materials Sciences Division interviews scientists about their lives and work in language everyone can understand. Her guest Berkeley Lab's MIke Crommie.

Tech Prep Marketing Guide. The Complete Book of Strategies and Practical Experiences.

ERIC Educational Resources Information Center

Williamson, Patty

This guide explains the concept of marketing tech prep and provides marketing principles and strategies to promote tech prep programs. The guide covers the following topics: (1) why it is necessary to market tech prep; (2) what a comprehensive tech prep marketing plan should include; (3) targeting the benefits message; (4) marketing tech prep to…

A Context Menu for the Real World: Controlling Physical Appliances Through Head-Worn Infrared Targeting

DTIC Science & Technology

2013-12-10

Edward A. Lee Björn Hartmann Electrical Engineering and Computer Sciences University of California at Berkeley Technical Report No. UCB/EECS-2013-200...NAME(S) AND ADDRESS(ES) University of California at Berkeley, Electrical Engineering and Computer Sciences,Berkeley,CA,94720 8. PERFORMING...movement. PHYSICAL TARGET ACQUISITION STUDY To understand the accuracy and performance of head- orientation-based selection through our device, we car - ried

A Context Menu for the Real World: Controlling Physical Appliances through Head-Worn Infrared Targeting

DTIC Science & Technology

2013-11-04

Edward A. Lee Björn Hartmann Electrical Engineering and Computer Sciences University of California at Berkeley Technical Report No. UCB/EECS-2013-182...NAME(S) AND ADDRESS(ES) University of California at Berkeley, Electrical Engineering and Computer Sciences,Berkeley,CA,94720 8. PERFORMING...accuracy and performance of head- orientation-based selection through our device, we car - ried out a comparative target acquisition study, where

Sit Down with Sabin: Margaret Torn: The Carbon Cycle Like You've Never Seen It (LBNL Summer Lecture Series)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Russell, Sabin; Torn, Margaret

2011-07-06

Lawrence Berkeley National Laboratory soil scientist Margaret Torn appears July 6, 2011 on "Sit Down with Sabin," a weekly conversation in which former reporter Sabin Russell chats with Berkeley Lab staff about innovative science. Torn discusses how she travels the world to learn more about soil's huge role in the global carbon cycle. Brought to you by Berkeley Lab Public Affairs.

An introduction to the nitrogen dynamics in controlled systems workshop. Life support and nitrogen: NASA's interest in nitrogen cycling

NASA Technical Reports Server (NTRS)

MacElroy, R. D.; Smernoff, D. T.

1996-01-01

A Workshop on "Nitrogen Dynamics in Controlled Systems" was held September 26-28, 1995 at the Lawrence Berkeley National Laboratory. The meetings were sponsored by the NASA Advanced Life Support program and the Lawrence Berkeley National Laboratory, and hosted by Prof. Lester Packer of the University of California at Berkeley, and of the Lawrence Berkeley National Laboratory. The Workshop participants were asked to: 1. summarize current knowledge on the cycling of nitrogen in closed systems; 2. identify the needs that closed systems may have for specific forms of nitrogen; 3. identify possible ways of generating and maintaining (or avoiding) specific forms and concentrations of nitrogen; 4. compare biological and physical/chemical methods of transforming nitrogen.

SemaTyP: a knowledge graph based literature mining method for drug discovery.

PubMed

Sang, Shengtian; Yang, Zhihao; Wang, Lei; Liu, Xiaoxia; Lin, Hongfei; Wang, Jian

2018-05-30

Drug discovery is the process through which potential new medicines are identified. High-throughput screening and computer-aided drug discovery/design are the two main drug discovery methods for now, which have successfully discovered a series of drugs. However, development of new drugs is still an extremely time-consuming and expensive process. Biomedical literature contains important clues for the identification of potential treatments. It could support experts in biomedicine on their way towards new discoveries. Here, we propose a biomedical knowledge graph-based drug discovery method called SemaTyP, which discovers candidate drugs for diseases by mining published biomedical literature. We first construct a biomedical knowledge graph with the relations extracted from biomedical abstracts, then a logistic regression model is trained by learning the semantic types of paths of known drug therapies' existing in the biomedical knowledge graph, finally the learned model is used to discover drug therapies for new diseases. The experimental results show that our method could not only effectively discover new drug therapies for new diseases, but also could provide the potential mechanism of action of the candidate drugs. In this paper we propose a novel knowledge graph based literature mining method for drug discovery. It could be a supplementary method for current drug discovery methods.

Concentration dependent requirement for local protein synthesis in motor neuron subtype specific response to axon guidance cues

PubMed Central

Nedelec, Stephane; Peljto, Mirza; Shi, Peng; Amoroso, Mackenzie W.; Kam, Lance C.; Wichterle, Hynek

2012-01-01

Formation of functional motor circuits relies on the ability of distinct spinal motor neuron subtypes to project their axons with high precision to appropriate muscle targets. While guidance cues contributing to motor axon pathfinding have been identified, the intracellular pathways underlying subtype specific responses to these cues remain poorly understood. In particular, it remains controversial whether responses to axon guidance cues depend on axonal protein synthesis. Using a growth cone collapse assay, we demonstrate that mouse embryonic stem cell (ESC) derived spinal motor neurons (ES-MNs) respond to ephrin-A5, Sema3f and Sema3a in a concentration dependent manner. At low doses, ES-MNs exhibit segmental or subtype specific responses, while this selectivity is lost at higher concentrations. Response to high doses of semaphorins and to all doses of ephrin-A5 is protein synthesis independent. In contrast, using microfluidic devices and stripe assays, we show that growth cone collapse and guidance at low concentrations of semaphorins relies on local protein synthesis in the axonal compartment. Similar bimodal response to low and high concentrations of guidance cues is observed in human ES-MNs, pointing to a general mechanism by which neurons increase their repertoire of responses to the limited set of guidance cues involved in neural circuit formation. PMID:22279234

UC Berkeley's Adaptations to the Crisis of Public Higher Education in the US: Privatization? Commercialization? or Hybridization? Research & Occasional Paper Series: CSHE.17.13

ERIC Educational Resources Information Center

Breslauer, George W.

2013-01-01

The University of California at Berkeley now delivers more to the public of California than it ever has, and it does this on the basis of proportionally less funding by the State government than it has ever received. This claim may come as a surprise, since it is often said that Berkeley is in the process of privatizing, becoming less of a public…

In Conversation with Jeff Neaton

ScienceCinema

Jeff Neaton

2017-12-09

Jan. 22, 2010: Host Alice Egan of Berkeley Lab's Materials Sciences Division interviews scientists about their lives and work in language everyone can understand. Her guest Berkeley Lab's MIke Crommie.

Putting Carbon in its Place: What You Can Do (LBNL Science at the Theater)

ScienceCinema

Walker, Iain; Regnier, Cindy [LBNL, Environmental Energy Technologies Division; Miller, Jeff; Masanet, Eric

2018-06-28

Science at the Theater: Berkeley Lab scientists reveal the latest research on how to reduce your carbon footprint at home, work, and when you shop. Learn how even small choices can have a big impact. Iain Walker's research focuses on optimizing the energy use and comfort of buildings. He's a staff scientist in the Energy Performance of Buildings Group, which is part of Berkeley Lab's Environmen...tal Energy Technologies Division. He's also executive editor of Home Energy Magazine. Cindy Regnier is a Project Manager in the Environmental Energy Technologies Division at Berkeley Lab. She has over 13 years of mechanical engineering design experience, with a focus on low-energy buildings. Her projects have included several LEED Platinum buildings and the design of a 200,000 sf carbon neutral, net-zero energy science museum in San Francisco. Eric Masanet is Acting Deputy Leader of the International Energy Studies Group at Berkeley Lab. His research focuses on life-cycle assessments and energy efficiency analysis. He holds a joint research appointment in the Institute of Transportation Studies at UC Berkeley.

Seeing the Light (LBNL Science at the Theater)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brunger, Axel; Segalman, Rachel; Westphal, Andrew

2011-09-12

Berkeley Lab's Science at the Theater event "Seeing the Light" took place on Sept 12, 2011, at Berkeley Repertory's Roda Theatre. Learn how the Advanced Light Source is improving medicine, paving the way for clean energy, changing the future of computers, and much more. Featured speakers are Berkeley Lab's Roger Falcone, Rachel Segalman, Andrew Westphal, and Stanford University's Axel Brunger. Rachel Segalman: The future of clean energy technology relies on a better understanding of materials at the nanoscale. Berkeley Lab's Rachel Segalman uses the ALS to conduct this research, which could lead to improved photovoltaics and fuel cells. Axel Brunger:more » Improved treatment for human diseases hinges on understanding molecular-scale processes. Stanford University's Axel Brunger will discuss a new melanoma drug that was developed by a local company, Plexxikon, using the ALS for X-ray data collection. Andrew Westphal: What's comet dust made of? Andrew Westphal of UC Berkeley's Space Sciences Laboratory uses the ALS to study comet dust and interplanetary space dust collected by a NASA spacecraft. Moderated by Roger Falcone, Division Director of the Advanced Light Source« less

In Conversation with Mike Crommie

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mike Crommie

2010-02-16

Dec. 9 2009: Host Alice Egan of Berkeley Lab's Materials Sciences Division interviews scientists about their lives and work in language everyone can understand. Her guest is Berkeley Lab's Mike Crommie.

In Conversation with Mike Crommie

ScienceCinema

Mike Crommie

2017-12-09

Dec. 9 2009: Host Alice Egan of Berkeley Lab's Materials Sciences Division interviews scientists about their lives and work in language everyone can understand. Her guest is Berkeley Lab's Mike Crommie.

Sneak Preview of April 25 Science at the Theater

ScienceCinema

Ho, Shirley

2017-12-12

Berkeley Lab astrophysicist Shirley Ho offers a sneak preview of the Science at the Theatre event at the Berkeley Repertory Theatre on April 25. Three Berkeley Lab cosmologists and Bay Area astronomer Andrew Fraiknoi will gather at the Berkeley Rep on Monday, April 25, from 7 to 9 p.m. to shed light on the mystery of the accelerating universe. Topics will include hunting down Type 1a supernovae, measuring the universe using baryon oscillation, and whether dark energy is the true driver of the universe. If you have questions for the scientists, post them below, send them to friendsofberkeleylab@lbl.gov, or catch us on facebook: http://www.facebook.com/video/video.php?v=10150215592292354&oid=593833429...Your question might be answered at the April 25 talk if there's time.

Novel Adult Stem Cells for Peripheral Nerve Regeneration

DTIC Science & Technology

2012-09-01

were also positive for MSC surface marker CD29 and CD44 (Fig. 1F-G). However, CD29 and CD44 are also expressed in SMCs, so we will not use these non...tubulin. In addition, MVSCs were negative for perivascular MSC marker CD146 (Fig. 1H) and SMC progenitor marker Sca-1 (Fig. 1I). MVSCs were also...University of California, Berkeley, California 94720, USA. 2 UC Berkeley-UCSF Graduate Program in Bioengineering, Berkeley, California 94720, USA. 3

Space Radiation and Cataracts (LBNL Summer Lecture Series)

ScienceCinema

Blakely, Eleanor [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division

2018-01-23

Summer Lecture Series 2009: Eleanor Blakely, radiation biologist of the Life Sciences Division at Lawrence Berkeley National Laboratory, has been a scientist at Berkeley Lab since 1975. She is studying the effect of radiation on cataracts which concerns not only cancer patients, but also astronauts. As astronauts spend increasingly longer time in space, the effects of cosmic radiation exposure will become an increasingly important health issue- yet there is little human data on these effects. Blakely reviews this emerging field and the contributions made at Berkeley Lab

UC Berkeley's Celebration of the International Year of Astronomy 2009

NASA Astrophysics Data System (ADS)

Cobb, B. E.; Croft, S.; Silverman, J. M.; Klein, C.; Modjaz, M.

2010-08-01

We present the astronomy outreach efforts undertaken for the International Year of Astronomy 2009 at the University of California, Berkeley. Our department-wide endeavors included a monthly public lecture series by UC Berkeley astronomers and a major astronomy outreach event during a campus-wide university "open house," which included solar observing and a Starlab Planetarium. In addition to sharing our outreach techniques and outcomes, we discuss some of our unique strategies for advertising our events to the local community.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Todd DeSantis

Scientists at Berkeley Lab and the University of California, Merced are using an innovative DNA array developed at Berkeley Lab to catalog the microbes that live among coral in the tropical waters off the coast of Puerto Rico.

Berkeley Lab Search - Search engine for Berkeley Lab

Science.gov Websites

twitter instagram google plus facebook youtube A U.S. Department of Energy National Laboratory Managed by the University of California Questions & Comments Privacy & Security Notice twitter instagram

ARC-2006-ACD06-0216-017

NASA Image and Video Library

2006-11-29

Stardust sample analysis @ UC Berkeley clean room with Dr Scott Sandford, NASA Ames Astrophysicist - mission samples provided to UC Berkeley for analysis by NASABerkeley researcher Zack Gainsforth working with sample encased in aerogel

ARC-2006-ACD06-0216-022

NASA Image and Video Library

2006-11-29

Stardust sample analysis @ UC Berkeley clean room with Dr Scott Sandford, NASA Ames Astrophysicist - mission samples provided to UC Berkeley for analysis by NASABerkeley researcher Zack Gainsforth working with sample encased in aerogel

Saul Perlmutter

Science.gov Websites

joined the UC Berkeley Physics Department in 2004. He is also an astrophysicist at Lawrence Berkeley Perlmutter won the Department of Energy's E. O. Lawrence Award in Physics. In 2003 he was awarded the

Sneak Preview of April 25 Science at the Theater

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ho, Shirley

Berkeley Lab astrophysicist Shirley Ho offers a sneak preview of the Science at the Theatre event at the Berkeley Repertory Theatre on April 25. Three Berkeley Lab cosmologists and Bay Area astronomer Andrew Fraiknoi will gather at the Berkeley Rep on Monday, April 25, from 7 to 9 p.m. to shed light on the mystery of the accelerating universe. Topics will include hunting down Type 1a supernovae, measuring the universe using baryon oscillation, and whether dark energy is the true driver of the universe. If you have questions for the scientists, post them below, send them to friendsofberkeleylab@lbl.gov, or catchmore » us on facebook: http://www.facebook.com/video/video.php?v=10150215592292354&oid=593833429...Your question might be answered at the April 25 talk if there's time.« less

The Texas Tech Prep Consortia: Strategies for Advancing Academic and Technical Education.

ERIC Educational Resources Information Center

Hensley, Oliver D., Ed.; And Others

This book contains 30 chapters on the tech prep initiative in Texas: "The Identity of Tech Prep in Texas" (Tunstall); "A Snap-Shot of the Impact of the Tech Prep Initiative in the Governor's 24 Planning Regions" (Brown); "The Tech Prep Consortium Directors: The Architects for the Future of Texas" (Hensley et al.);…

Tech Prep Implementation in the United States: The Once and Future Role of Community Colleges.

ERIC Educational Resources Information Center

Bragg, Debra D.; Layton, James D.

1995-01-01

Describes a study examining the impact of the Tech Prep Education Act on the initial implementation of federally supported Tech Prep Initiatives. Based on telephone survey responses from all 50 state Tech Prep leaders, reviews findings concerning the funding of Tech Prep consortia, state and local administrative structures, policies and goals, and…

Tech-Prep in New York State: Profiles of Four Diverse Programs.

ERIC Educational Resources Information Center

Frenkel, Michael W.; Brodsky, Stanley M.

These case studies highlight the diversity of four tech programs that responded with a unique set of organizational policies and procedures to a unique set of challenges. The case study on City Tech Tech-Prep Consortium in Brooklyn focuses on three strategies: transition to City Tech program, postsecondary component, and program evaluation. The…

Harold F. Weaver: California Astronomer

NASA Astrophysics Data System (ADS)

Shields, J. C.

1993-05-01

This talk will give an overview of an oral history recently completed with Harold F. Weaver, Professor Emeritus of Astronomy at the University of California at Berkeley. Weaver grew up in California and studied as an undergraduate at Berkeley, where he also pursued graduate work incorporating research at Lick and Mount Wilson Observatories. After pursuing postdoctoral research at Yerkes Observatory and war work in Cambridge (Massachusetts) and Berkeley, Weaver was appointed to the staff of Lick Observatory. In 1951 he joined the faculty at Berkeley, where he later played a major role in founding Hat Creek Radio Observatory. As Director of the Berkeley Radio Astronomy Laboratory, Weaver oversaw construction of the 85-foot telescope at Hat Creek, which is the subject of a special session at this meeting. Two aspects of Weaver's career will be highlighted. The first is the somewhat unusual and very successful transition in Weaver's observational research from emphasis on classical photographic techniques at optical wavelengths to use of emerging radio technology for the study of Galactic structure. The second is service provided by Weaver to the American Astronomical Society and Astronomical Society of the Pacific at several key junctures in the development of both organizations.

Ernest Orlando Lawrence Berkeley National Laboratory Institutional Plan FY 2000-2004

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chartock, Mike; Hansen, Todd

1999-08-01

The FY 2000-2004 Institutional Plan provides an overview of the Ernest Orlando Lawrence Berkeley National Laboratory (Berkeley Lab, the Laboratory) mission, strategic plan, initiatives, and the resources required to fulfill its role in support of national needs in fundamental science and technology, energy resources, and environmental quality. To advance the Department of Energy's ongoing efforts to define the Integrated Laboratory System, the Berkeley Lab Institutional Plan reflects the strategic elements of our planning efforts. The Institutional Plan is a management report that supports the Department of Energy's mission and programs and is an element of the Department of Energy's strategicmore » management planning activities, developed through an annual planning process. The Plan supports the Government Performance and Results Act of 1993 and complements the performance-based contract between the Department of Energy and the Regents of the University of California. It identifies technical and administrative directions in the context of the national energy policy and research needs and the Department of Energy's program planning initiatives. Preparation of the plan is coordinated by the Office of Planning and Communications from information contributed by Berkeley Lab's scientific and support divisions.« less

Joint SSRTNet/ALS-MES Workshop report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shuh, David; Van Hove, Michel

2001-11-30

This joint workshop brought together experimentalists and theorists interested in synchrotron radiation and highlighted subjects relevant to molecular environmental science (MES). The strong mutual interest between the participants resulted in joint sessions on the first day, followed by more specialized parallel sessions on the second day. Held in conjunction with the Advanced Light Source (ALS) Users' Association Annual Meeting at the Lawrence Berkeley National Laboratory (Berkeley Lab), the Synchrotron Radiation Research Theory Network (SRRTNet) workshop was co-organized by Michel Van Hove (Berkeley Lab and University of California, Davis) and Andrew Canning (Berkeley Lab), while David Shuh (Berkeley Lab) organized themore » ALS-MES workshop. SRRTNet is a global network that promotes the interaction of theory and experiment (http://www.cse.clrc.ac.uk/Activity/SRRTnet). The ALS-MES project is constructing Beamline 11.0.2.1-2, a new soft x-ray beamline for MES investigations at photon energies from 75 eV to 2 keV, to provide photons for wet spectroscopy end stations and an upgraded scanning transmission x-ray microscope (STXM). The ALS-MES beamline and end stations will be available for users in the late fall of 2002.« less

Gerson Goldhaber: A Life in Science

NASA Astrophysics Data System (ADS)

Pavlish, Ursula

2011-06-01

I draw on my interviews in 2005-2007 with Gerson Goldhaber (1924-2010), his wife Judith, and his colleagues at Lawrence Berkeley National Laboratory. I discuss his childhood, early education, marriage to his first wife Sulamith (1923-1965), and his further education at the Hebrew University in Jerusalem (1942-1947) and his doctoral research at University of Wisconsin at Madison (1947-1950). He then was appointed to an instructorship in physics at Columbia University (1950-1953) before accepting a position in the physics department at the University of California at Berkeley and the Radiation Laboratory (later the Lawrence Berkeley Laboratory, today the Lawrence Berkeley National Laboratory), where he remained for the rest of his life. He made fundamental contributions to physics, including to the discovery of the antiproton in 1955, the GGLP effect in 1960, the psi particle in 1974, and charmed mesons in 1977, and to cosmology, including the discovery of the accelerating universe and dark energy in 1998. Beginning in the late 1960s, he also took up art, and he and his second wife Judith, whom he married in 1969, later collaborated in illustrating and writing two popular books. Goldhaber died in Berkeley, California, on July 19, 2010, at the age of 86.

PhyloChip Tackles Coral Disease

ScienceCinema

Todd DeSantis

2017-12-09

Scientists at Berkeley Lab and the University of California, Merced are using an innovative DNA array developed at Berkeley Lab to catalog the microbes that live among coral in the tropical waters off the coast of Puerto Rico.

Berkeley Lab Scientists Recipients of 2015 Breakthrough Prizes | Berkeley

Science.gov Websites

. Doudna and Charpentier have been at the forefront of research into a genetic element known as CRISPR , which stands for Clustered Regularly Interspaced Short Palindromic Repeats. The combination of CRISPR

Hot Technology, Cool Science (LBNL Science at the Theater)

ScienceCinema

Fowler, John

2018-06-08

Great innovations start with bold ideas. Learn how Berkeley Lab scientists are devising practical solutions to everything from global warming to how you get to work. On May 11, 2009, five Berkeley Lab scientists participated in a roundtable dicussion moderated by KTVU's John Fowler on their leading-edge research. This "Science at the Theater" event, held at the Berkeley Repertory Theatre, featured technologies such as cool roofs, battery-driven transportation, a pocket-sized DNA probe, green supercomputing, and a noncontact method for restoring damaged and fragile mechanical recordings.

BEARS: Radioactive Ion Beams at Berkeley

DOE Office of Scientific and Technical Information (OSTI.GOV)

Powell, J.; Joosten, R.; Donahue, C.A.

2000-03-14

A light-isotope radioactive ion beam capability has been added to the 88-Inch Cyclotron at Lawrence Berkeley National Laboratory by coupling to the production cyclotron of the Berkeley Isotope Facility. The connection required the development and construction of a 350 m gas transport system between the two accelerators as well as automated cryogenic separation of the produced activity. The first beam developed, {sup 11}C, has been successfully accelerated with an on-target intensity of 1 x 10{sup 8} ions/sec at energies of around 10 MeV/u.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Urban, Jeff

Berkeley Lab materials scientist, Jeff Urban presents his research on using metal-organic frameworks to capture carbon at Berkeley Lab's Cleantech Pitchfest on June 1, 2016. Removing excess carbon from an overheating atmosphere is an urgent and complicated problem. The answer, according to Berkeley Lab’s Jeff Urban, could lie at the nanoscale, where specially designed cage-like structures called metal organic frameworks, or MOFs, can trap large amounts of carbon in microscopically tiny structures. A Harvard PhD with expertise in thermoelectrics, gas separation and hydrogen storage, Urban directs teams at the Molecular Foundry’s Inorganic Materials Facility.

Tech Prep Implementation and Preliminary Student Outcomes for Eight Local Tech Prep Consortia.

ERIC Educational Resources Information Center

Bragg, Debra D.; Dare, Donna E.; Reger, W. M., IV; Ovaice, Ghazala; Zamani, Eboni M.; Layton, James D.; Dornsife, Carolyn J.; Vallee, Manuel; Brown, Carrie H.; Orr, Margaret Terry

The implementation and student outcomes of Tech Prep were examined in a study of eight consortia that represented a range of Tech Prep models and approaches in urban, suburban, and rural locations across the United States. Data were collected from the following sources: field visits; follow-up survey of Tech Prep participants and nonparticipants;…

Water and organics in interplanetary dust particles

NASA Astrophysics Data System (ADS)

Bradley, John P.

2015-08-01

Interplanetary dust particles (IDPs) and larger micrometeorites (MMs) impinge on the upper atmosphere where they decelerate at ~90 km altitude and settle to the Earth’s surface. Comets and asteroids are the major sources and the flux, 30,000-40,000 tons/yr, is comparable to the mass of larger meteorites impacting the Earth’s surface. The sedimentary record suggests that the flux was much higher on the early Earth. The chondritic porous (CP) subset of IDPs together with their larger counterparts, ultracarbonaceous micrometeorites (UCMMs), appear to be unique among known meteoritic materials in that they are composed almost exclusively of anhydrous minerals, some of them contain >> 50% organic carbon by volume as well as the highest abundances of presolar silicate grains including GEMS. D/H and 15N abundances implicate the Oort Cloud or presolar molecular cloud as likely sources of the organic carbon. Prior to atmospheric entry, IDPs and MMs spend ~104-105 year lifetimes in solar orbit where their surfaces develop amorphous space weathered rims from exposure to the solar wind (SW). Similar rims are observed on lunar soil grains and on asteroid Itokawa regolith grains. Using valence electron energy-loss spectroscopy (VEELS) we have detected radiolytic water in the rims on IDPs formed by the interaction of solar wind protons with oxygen in silicate minerals. Therefore, IDPs and MMs continuously deliver both water and organics to the earth and other terrestrial planets. The interaction of protons with oxygen-rich minerals to form water is a universal process.Affiliations:a University of Hawaii at Manoa, Hawaii Institute of Geophysics and Planetology, 1680 East-West Road, Honolulu, HI 96822, USA.b National Center for Electron Microscopy, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.c Materials Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.d Department of Materials Science & Engineering, University of California, Berkeley, CA 94720, USA.e Advanced Light Source Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Beyond SWEAT: Developing Infrastructure in Stability and COIN Operations

DTIC Science & Technology

2011-10-05

respecting their desire for self-determination. See Pressman , Jeffrey L. and Wildavsky, Aaron. Implementation. (Berkeley: University of California Press... Pressman , Jeffrey L. and Wildavsky, Aaron. Implementation. (Berkeley: University of California Press 1971). Sablan, Gregory. Developing

Summer Series 2012 - Conversation with Kathy Yelick

ScienceCinema

Yelick, Kathy, Miller, Jeff

2018-05-11

Jeff Miller, head of Public Affairs, sat down in conversation with Kathy Yelick, Associate Berkeley Lab Director, Computing Sciences, in the second of a series of powerpoint-free talks on July 18th 2012, at Berkeley Lab.

Theory-based Bayesian Models of Inductive Inference

DTIC Science & Technology

2010-07-19

Subjective randomness and natural scene statistics. Psychonomic Bulletin & Review . http://cocosci.berkeley.edu/tom/papers/randscenes.pdf Page 1...in press). Exemplar models as a mechanism for performing Bayesian inference. Psychonomic Bulletin & Review . http://cocosci.berkeley.edu/tom

Summer Series 2012 - Conversation with Kathy Yelick

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yelick, Kathy, Miller, Jeff

2012-07-23

Jeff Miller, head of Public Affairs, sat down in conversation with Kathy Yelick, Associate Berkeley Lab Director, Computing Sciences, in the second of a series of powerpoint-free talks on July 18th 2012, at Berkeley Lab.

Berkeley bicycle plan : draft for inclusion in the general plan

DOT National Transportation Integrated Search

1998-12-31

The City of Berkeley has long supported bicycling as an environmentally friendly, healthy, lowcost method of transportation and recreation. Frequently, roadway facility and funding decisions are made with little consideration for bicycling as a serio...

Berkeley Lab 2nd Grader Outreach

ScienceCinema

Scoggins, Jackie; Louie, Virginia

2017-12-11

The Berkeley Lab IT Department sponsored a community outreach program aimed at teaching young children about computers and networks. Second graders from LeConte Elementary School joined Lab IT Staff for a day of in-depth exercises and fun.

Analysis, tuning and comparison of two general sparse solvers for distributed memory computers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Amestoy, P.R.; Duff, I.S.; L'Excellent, J.-Y.

2000-06-30

We describe the work performed in the context of a Franco-Berkeley funded project between NERSC-LBNL located in Berkeley (USA) and CERFACS-ENSEEIHT located in Toulouse (France). We discuss both the tuning and performance analysis of two distributed memory sparse solvers (superlu from Berkeley and mumps from Toulouse) on the 512 processor Cray T3E from NERSC (Lawrence Berkeley National Laboratory). This project gave us the opportunity to improve the algorithms and add new features to the codes. We then quite extensively analyze and compare the two approaches on a set of large problems from real applications. We further explain the main differencesmore » in the behavior of the approaches on artificial regular grid problems. As a conclusion to this activity report, we mention a set of parallel sparse solvers on which this type of study should be extended.« less

Site Environmental Report for 2011, Volumes 1& 2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Baskin, David; Bauters, Tim; Borglin, Ned

2012-09-12

The Site Environmental Report for 2011 summarizes Berkeley Lab’s environmental management performance, presents environmental monitoring results, and describes significant programs for calendar year (CY) 2011. Throughout this report, “Berkeley Lab” or “LBNL” refers both to (1) the multiprogram scientific facility the UC manages and operates on the 202-acre university-owned site located in the hills above the UC Berkeley campus, and the site itself, and (2) the UC as managing and operating contractor for Ernest Orlando Lawrence Berkeley National Laboratory. The report is separated into two volumes. Volume I is organized into an executive summary followed by six chapters that includemore » an overview of LBNL, a discussion of its Environmental Management System (EMS), the status of environmental programs, summarized results from surveillance and monitoring activities, and quality assurance (QA) measures. Volume II contains individual data results from surveillance and monitoring activities.« less

Spatio-Temporal Nonlinear Filtering With Applications to Information Assurance and Counter Terrorism

DTIC Science & Technology

2011-11-14

2009): 279. doi: 10.1016/j.comnet.2008.10.001 2011/11/07 14:36:13 44 Jelena Mirkovic, Peter Reiher, Christos Papadopoulos, Alefiya Hussain, Marla Shepard ...Applications to Remote Sensing,” Department of Statistics and Department of Computer Sciences, University of Chicago , September, 2011 (Invited). 2. A.G... Chicago , IL April 13, 2010, Edward H. Bosch Organizer. 58. Andrea Bertozzi, Invited talk, Plenary talk, Joint SIAM/RSME-SCM-SEMA Meeting on Emerging

The FLOW Tutor: Schemas for Tutoring.

DTIC Science & Technology

1977-05-01

1 ~~ Donald R. Gentner Donald A . Norm an THE FLOW TUTOR: SCHEM AS FOR TUTORING H D D C ss~~~ UNIVERSiTY OF CALIFO RNIA , SAN DIEGO LA JOLLA...individualized in— struction , instructional theory, model of the student , representation of information , sc ’ nema , sema ntic ne twork , teaching...I ~~r giving adv ice to a studen t learning ~ simp1e 1~computer language .The tutor has a schema—based knowledge structure

Identification of neuron-related genes for cell therapy of neurological disorders by network analysis.

PubMed

Su, Li-Ning; Song, Xiao-Qing; Wei, Hui-Ping; Yin, Hai-Feng

Bone mesenchymal stem cells (BMSCs) differentiated into neurons have been widely proposed for use in cell therapy of many neurological disorders. It is therefore important to understand the molecular mechanisms underlying this differentiation. We screened differentially expressed genes between immature neural tissues and untreated BMSCs to identify the genes responsible for neuronal differentiation from BMSCs. GSE68243 gene microarray data of rat BMSCs and GSE18860 gene microarray data of rat neurons were received from the Gene Expression Omnibus database. Transcriptome Analysis Console software showed that 1248 genes were up-regulated and 1273 were down-regulated in neurons compared with BMSCs. Gene Ontology functional enrichment, protein-protein interaction networks, functional modules, and hub genes were analyzed using DAVID, STRING 10, BiNGO tool, and Network Analyzer software, revealing that nine hub genes, Nrcam, Sema3a, Mapk8, Dlg4, Slit1, Creb1, Ntrk2, Cntn2, and Pax6, may play a pivotal role in neuronal differentiation from BMSCs. Seven genes, Dcx, Nrcam, sema3a, Cntn2, Slit1, Ephb1, and Pax6, were shown to be hub nodes within the neuronal development network, while six genes, Fgf2, Tgfβ1, Vegfa, Serpine1, Il6, and Stat1, appeared to play an important role in suppressing neuronal differentiation. However, additional studies are required to confirm these results.

In Conversation With Materials Scientist Ron Zuckermann

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ron Zuckerman

2009-11-18

Nov. 11, 2009: Host Alice Egan of Berkeley Lab's Materials Sciences Division interviews scientists about their lives and work in language everyone can understand. Her guest Berkeley Lab's Ron Zuckerman, who discusses biological nanostructures and the world of peptoids.

Early Geologic Education in California--Berkeley and Stanford Show the Way.

ERIC Educational Resources Information Center

Norris, Robert M.

1981-01-01

Traces the early history of geological education in California universities, with emphasis upon programs at Berkeley and Stanford. Among the pioneers in the field were Joseph LeConte, Andrew C. Lawson, and John C. Branner. (WB)

In Conversation With Materials Scientist Ron Zuckermann

ScienceCinema

Ron Zuckerman

2017-12-09

Nov. 11, 2009: Host Alice Egan of Berkeley Lab's Materials Sciences Division interviews scientists about their lives and work in language everyone can understand. Her guest Berkeley Lab's Ron Zuckerman, who discusses biological nanostructures and the world of peptoids.

NASA Tech Briefs Index 1979. Volume 4, Nos. 1-4

NASA Technical Reports Server (NTRS)

1980-01-01

Tech Briefs are short announcements of new technology derived from the research and development activities of the National Aeronautics and Space Administration. These briefs emphasize information considered likely to be transferrable across industrial, regional, or disciplinary lines and are issued to encourage commercial application. This Index to NASA Tech Briefs contains abstracts and four indexes -- subject, personal author, originating Center, and Tech Brief number -- for 1979 Tech Briefs.

NASA Tech Briefs Index 1978. Volume 3, Nos. 1-4

NASA Technical Reports Server (NTRS)

1978-01-01

Tech Briefs are short announcements of new technology derived from the research and development activities of the National Aeronautics and Space Administration. These briefs emphasize information considered likely to be transferrable across industrial, regional, or disciplinary lines and are issued to encourage commercial application. This Index to NASA Tech Briefs contains abstracts and four indexes -- subject, personal author, originating Center, and Tech Brief number -- for 1978 Tech Briefs.

NASA Tech Briefs Index 1980. Volume 5, Nos. 1-4

NASA Technical Reports Server (NTRS)

1981-01-01

Tech Briefs are short announcements of new technology derived from the research and development activities of the National Aeronautics and Space Administration. These briefs emphasize information considered likely to be transferrable across industrial, regional, or disciplinary lines and are issued to encourage commercial application. This Index to NASA Tech Briefs contains abstracts and four indexes -- subject,. personal author, originating Center, and Tech Brief number -- for 1980 Tech Briefs.

Cumulative index to NASA Tech Briefs, 1970-1975. [bibliographies

NASA Technical Reports Server (NTRS)

1979-01-01

Tech briefs of technology derived from the research and development activities of the National Aeronautics and Space Administration are presented. Abstracts and indexes of subject, personal author, originating center, and tech brief number for the 1970-1975 tech briefs are presented.

What is Supercomputing? A Conversation with Kathy Yelick

ScienceCinema

Yelick, Kathy

2017-12-11

In this highlight video, Jeff Miller, head of Public Affairs, sat down in conversation with Kathy Yelick, Associate Berkeley Lab Director, Computing Sciences, in the second of a series of "powerpoint-free" talks on July 18th 2012, at Berkeley Lab.

What is Supercomputing? A Conversation with Kathy Yelick

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yelick, Kathy

2012-07-23

In this highlight video, Jeff Miller, head of Public Affairs, sat down in conversation with Kathy Yelick, Associate Berkeley Lab Director, Computing Sciences, in the second of a series of "powerpoint-free" talks on July 18th 2012, at Berkeley Lab.

LBNL Computational ResearchTheory Facility Groundbreaking - Full Press Conference. Feb 1st, 2012

ScienceCinema

Yelick, Kathy

2018-01-24

Energy Secretary Steven Chu, along with Berkeley Lab and UC leaders, broke ground on the Lab's Computational Research and Theory (CRT) facility yesterday. The CRT will be at the forefront of high-performance supercomputing research and be DOE's most efficient facility of its kind. Joining Secretary Chu as speakers were Lab Director Paul Alivisatos, UC President Mark Yudof, Office of Science Director Bill Brinkman, and UC Berkeley Chancellor Robert Birgeneau. The festivities were emceed by Associate Lab Director for Computing Sciences, Kathy Yelick, and Berkeley Mayor Tom Bates joined in the shovel ceremony.

LBNL Computational Research and Theory Facility Groundbreaking. February 1st, 2012

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yelick, Kathy

2012-02-02

Energy Secretary Steven Chu, along with Berkeley Lab and UC leaders, broke ground on the Lab's Computational Research and Theory (CRT) facility yesterday. The CRT will be at the forefront of high-performance supercomputing research and be DOE's most efficient facility of its kind. Joining Secretary Chu as speakers were Lab Director Paul Alivisatos, UC President Mark Yudof, Office of Science Director Bill Brinkman, and UC Berkeley Chancellor Robert Birgeneau. The festivities were emceed by Associate Lab Director for Computing Sciences, Kathy Yelick, and Berkeley Mayor Tom Bates joined in the shovel ceremony.

LBNL Computational Research and Theory Facility Groundbreaking. February 1st, 2012

ScienceCinema

Yelick, Kathy

2017-12-09

Energy Secretary Steven Chu, along with Berkeley Lab and UC leaders, broke ground on the Lab's Computational Research and Theory (CRT) facility yesterday. The CRT will be at the forefront of high-performance supercomputing research and be DOE's most efficient facility of its kind. Joining Secretary Chu as speakers were Lab Director Paul Alivisatos, UC President Mark Yudof, Office of Science Director Bill Brinkman, and UC Berkeley Chancellor Robert Birgeneau. The festivities were emceed by Associate Lab Director for Computing Sciences, Kathy Yelick, and Berkeley Mayor Tom Bates joined in the shovel ceremony.

Blasting Rocks and Blasting Cars Applied Engineering

ScienceCinema

LBNL

2017-12-09

June 30, 2004 Berkeley Lab lecture: Deb Hopkins works with industries like automobile, mining and paper to improve their evaluation and measuring techniques. For several years, she has coordinated ... June 30, 2004 Berkeley Lab lecture: Deb Hopkins works with industries like automobile, mining and paper to improve their evaluation and measuring techniques. For several years, she has coordinated a program at Berkeley Lab funded under the Partnership for a New Generation of Vehicles, a collaboration between the federal government and the U.S. Council for Automotive Research. Nondestructive evaluation techniques to test a car's structural integrity are being developed for auto assembly lines.

Research on the competitiveness of high-tech industries in northeast China

NASA Astrophysics Data System (ADS)

Liu, Lei; Wang, Yang; Wang, Ming-Quan; Xiao, Yan-Bo; Gao, Ming

2017-06-01

Based on characteristics of high-tech industry in Northeast China, high-tech industry competitiveness index system was developed, and the competitiveness of high-tech industry was evaluated through principal component analysis and location quotient method. The results showed that the scale of high-tech industry in Northeast China as a whole was small, and presented a decreasing trend for the proportion in the country. The competitiveness of high-tech industry in Northeast China lagged far behind that of the eastern and central regions. The high-tech industry competitiveness of Liaoning, Jilin and Heilongjiang provinces in China ranked 15, 19 and 21, respectively. The manufacture of medicine in Jilin province, and the manufacture of aircraft and spacecraft and the related equipment in Liaoning and Heilongjiang provinces had high competitive advantage, but the manufacture of electronic equipment and communication equipment and the manufacture of computers and office equipment was lack of competitiveness. The development suggestions were put forward to improve the competitiveness of high-tech industries in Northeast China.

DOE Office of Scientific and Technical Information (OSTI.GOV)

DeSantis, Todd

Scientists at Berkeley Lab and the University of California, Merced are using an innovative DNA array developed at Berkeley Lab to catalog the microbes that live among coral in the tropical waters off the coast of Puerto Rico. More info: http://newscenter.lbl.gov/feature-stories/2009/02/02/coral-reefs/

Smart parking Value Pricing Pilot Project (VPPP) on the COASTER commuter rail line in San Diego, California.

DOT National Transportation Integrated Search

2012-03-01

This study provides a report on the Smart Parking Pilot, which was a partnership among the California Department of Transportation (Caltrans); the Institute of Transportation Studies- Berkeley's (ITS-Berkeley) Transportation Sustainability Research C...

77 FR 75639 - National Cancer Institute Notice of Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-21

... DEPARTMENT OF HEALTH AND HUMAN SERVICES National Institutes of Health National Cancer Institute... Proposed Frederick National Laboratory for Cancer Research Strategic Plan. Place: The Lawrence Berkeley..., Berkeley, CA 94720. Contact Person: Thomas M. Vollberg, Sr., Ph.D., Executive Secretary, National Cancer...

Carbon Smackdown: Smart Windows (LBNL Summer Lecture Series)

ScienceCinema

Milliron, Delia; Selkowitz, Stephen

2017-12-09

August 3, 2010 Berkeley Lab talk: In the fourth of five Carbon Smackdown matches, Berkeley Lab researchers Delia Milliron of the Materials Sciences Division and Stephen Selkowitz of the Environmental Energy Technologies Division talk about their work on energy-saving smart windows.

Illinois Tech Prep Planning Strategies.

ERIC Educational Resources Information Center

Illinois Univ., Urbana. Dept. of Vocational and Technical Education.

This tech prep planning handbook is based on the research conducted at the Office of Community College Research and Leadership, University of Illinois at Urbana-Champaign. The study involved information gathering procedures at each of the 17 tech prep pilot sites about their planning activities. Seven sections are included: (1) tech prep in…

Wide-Bandgap MOSFET Research with Virginia Tech Graduate Students |

Science.gov Websites

Advanced Manufacturing Research | NREL Wide Bandgap MOSFET Research with Virginia Tech Wide -Bandgap MOSFET Research with Virginia Tech Graduate Students Along with graduate student fellows from Virginia Tech, NREL is researching aspects related to the reliability and prognostics of power electronic

Earphone English.

ERIC Educational Resources Information Center

Goldsmith, Francisca

2002-01-01

Describes Earphone English, a student club sponsored through a partnership between Berkeley High School and the Berkeley Public Library that offers students whose primary language is not English to practice their spoken and aural English skills. Discusses the audiobooks used in the program and the importance of multicultural content and age…

The Universe Adventure - Credits

Science.gov Websites

Basel), and George Smoot (LBNL) Content, Graphic/Web Design Artie Konrad (student, UC Berkeley) 2004 Berkeley) Laurie Kerrigan (teacher, Mercy High School) Graphic/Web Design Melissa McClure (student ) Graphic/Web Design Paul Higgins (student, Contra Costa College) Other Gordon Aubrecht (Ohio State

Reimagining Educational Research: A Conversation

ERIC Educational Resources Information Center

Carter, Prudence L.; Nasir, Na'ilah Suad

2017-01-01

The following is a transcript of an interview between the Dean of the University of California (UC) Berkeley's Graduate School of Education, Prudence Carter, and UC Berkeley's outgoing Vice Chancellor for Equity and Inclusion, Na'ilah Suad Nasir, recorded at the 2017 Graduate School of Education Research Day.

Alessandra Lanzara

Science.gov Websites

Berkeley Lab Berkeley Lab A-Z Index Phone Book Jobs Search DOE Search MSD Go MSD - Materials Investigators Division Staff Facilities and Centers Staff Jobs Safety Personnel Resources Committees In Case of ; Finance Templates Travel One-Stop Investigators Division Staff Facilities and Centers Staff Jobs

Obtaining Parts

Science.gov Websites

The Cosmic Connection Parts for the Berkeley Detector Suppliers: Scintillator Eljen Technology 1 obtain the components needed to build the Berkeley Detector. These companies have helped previous the last update. He estimates that the cost to build a detector varies from $1500 to $2700 depending

NIEHS/EPA CEHCs: Berkeley/Stanford Children's Environment Health Center - UC Berkeley

EPA Pesticide Factsheets

The goal of this Center is to better understand the effects of exposure in the womb to air pollutants and airborne bacteria on newborn health, immune system health during childhood, and to understand the relationship of these early-life exposures to asthma

Berkeley Lab's Saul Perlmutter Wins the Einstein Medal | Berkeley Lab

Science.gov Websites

TAGS: awards, cosmology and astrophysics, physics Connect twitter instagram LinkedIn facebook youtube Physics + Cosmology Chemistry + Materials Sciences twitter instagram LinkedIn facebook youtube A U.S Privacy & Security Notice twitter instagram LinkedIn facebook youtube

Taxi Regulatory Revision in Oakland and Berkeley, California : Two Case Studies

DOT National Transportation Integrated Search

1983-06-01

A case study was performed of regulatory changes pertaining to taxicabs in Oakland and neighboring Berkeley, CA. In Oakland rates were approximately doubled and limits on the number of taxi permits removed. Companies were allowed to levy a per-trip s...

40 CFR 81.341 - South Carolina.

Code of Federal Regulations, 2012 CFR

2012-07-01

... County X Anderson County X Bamberg County X Barnwell County X Beaufort County X Berkeley County X Calhoun... County X Bamberg County X Barnwell County X Beaufort County X Berkeley County X Calhoun County X... Calhoun County Charleston County Cherokee County Chester County Chesterfield County Clarendon County...

40 CFR 81.341 - South Carolina.

Code of Federal Regulations, 2013 CFR

2013-07-01

... County X Anderson County X Bamberg County X Barnwell County X Beaufort County X Berkeley County X Calhoun... County X Bamberg County X Barnwell County X Beaufort County X Berkeley County X Calhoun County X... Calhoun County Charleston County Cherokee County Chester County Chesterfield County Clarendon County...

Comprehensive facilities plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1997-09-01

The Ernest Orlando Lawrence Berkeley National Laboratory`s Comprehensive Facilities Plan (CFP) document provides analysis and policy guidance for the effective use and orderly future development of land and capital assets at the Berkeley Lab site. The CFP directly supports Berkeley Lab`s role as a multiprogram national laboratory operated by the University of California (UC) for the Department of Energy (DOE). The CFP is revised annually on Berkeley Lab`s Facilities Planning Website. Major revisions are consistent with DOE policy and review guidance. Facilities planing is motivated by the need to develop facilities for DOE programmatic needs; to maintain, replace and rehabilitatemore » existing obsolete facilities; to identify sites for anticipated programmatic growth; and to establish a planning framework in recognition of site amenities and the surrounding community. The CFP presents a concise expression of the policy for the future physical development of the Laboratory, based upon anticipated operational needs of research programs and the environmental setting. It is a product of the ongoing planning processes and is a dynamic information source.« less

A QUANTITATIVE ANALYSIS OF DISTANT OPEN CLUSTERS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Janes, Kenneth A.; Hoq, Sadia

2011-03-15

The oldest open star clusters are important for tracing the history of the Galactic disk, but many of the more distant clusters are heavily reddened and projected against the rich stellar background of the Galaxy. We have undertaken an investigation of several distant clusters (Berkeley 19, Berkeley 44, King 25, NGC 6802, NGC 6827, Berkeley 52, Berkeley 56, NGC 7142, NGC 7245, and King 9) to develop procedures for separating probable cluster members from the background field. We next created a simple quantitative approach for finding approximate cluster distances, reddenings, and ages. We first conclude that with the possible exceptionmore » of King 25 they are probably all physical clusters. We also find that for these distant clusters our typical errors are about {+-}0.07 in E(B - V), {+-}0.15 in log(age), and {+-}0.25 in (m - M){sub o}. The clusters range in age from 470 Myr to 7 Gyr and range from 7.1 to 16.4 kpc from the Galactic center.« less

Planning a Tech Prep Program in Hospitality. Final Report.

ERIC Educational Resources Information Center

Chicago City Colleges, IL. Chicago City-Wide Coll.

A project conducted to establish tech prep articulation training agreements between City Colleges of Chicago and several public secondary schools is described in this report. Project activities were as follows: (1) developed a handbook to define the tech prep experience and guide committee members on how tech prep can be a valuable asset to…

Evaluating and Improving Tech Prep: Development, Validation, and Results of the Minnesota Self-Assessment Model.

ERIC Educational Resources Information Center

Pucel, David J.; And Others

1996-01-01

The Minnesota Tech Prep Self-Evaluation System is a framework based on ideas from Deming, Juran, and the Baldridge award. Testing with 17 Minnesota tech prep consortia found it effective in identifying areas needing improvement and promoting dialog among those involved in tech prep implementation. (SK)

Tech-Prep Competency Profiles within the Engineering Technologies Cluster.

ERIC Educational Resources Information Center

Ohio State Univ., Columbus. Center on Education and Training for Employment.

This document contains 12 competency profiles for tech prep courses within the engineering technologies cluster. The document consists of the following sections: (1) systemic curriculum reform philosophy--Ohio's vision of tech prep and its six critical components; (2) an explanation of the process of developing the tech prep competencies; (3) a…

34 CFR 406.1 - What is the State-Administered Tech-Prep Education Program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 3 2011-07-01 2011-07-01 false What is the State-Administered Tech-Prep Education... (Continued) OFFICE OF VOCATIONAL AND ADULT EDUCATION, DEPARTMENT OF EDUCATION STATE-ADMINISTERED TECH-PREP EDUCATION PROGRAM General § 406.1 What is the State-Administered Tech-Prep Education Program? If the annual...

What's Right with Kansas? (LBNL Science at the Theater)

ScienceCinema

Fuller, Merrian; Jackson, Nancy

2018-06-20

On Monday, Oct. 3 at 7 p.m. in Berkeley's Repertory Theater, the Lab presented "What's Right with Kansas," an evening of conversation with the Kansas-based Climate and Energy Project's founder and board chair, Nancy Jackson, and Berkeley Lab scientist Merrian Fuller, an electricity-market, policy and consumer behavior expert. Berkeley Lab will also debut its video "Common Ground," which showcases how CEP has become a Kansas mainstay and an inspiration to environmental organizations across the country. In a state rife with climate-change skepticism, CEP has changed behavior, and some minds, by employing rural values of thrift, independence, conservation, and friendly competition to promote energy efficiency.

SF Cleantech Pitchfest: Nano Sponges for Carbon Capture

ScienceCinema

Urban, Jeff

2018-01-16

Berkeley Lab materials scientist, Jeff Urban presents his research on using metal-organic frameworks to capture carbon at Berkeley Lab's Cleantech Pitchfest on June 1, 2016. Removing excess carbon from an overheating atmosphere is an urgent and complicated problem. The answer, according to Berkeley Lab’s Jeff Urban, could lie at the nanoscale, where specially designed cage-like structures called metal organic frameworks, or MOFs, can trap large amounts of carbon in microscopically tiny structures. A Harvard PhD with expertise in thermoelectrics, gas separation and hydrogen storage, Urban directs teams at the Molecular Foundry’s Inorganic Materials Facility.

Blasting Rocks and Blasting Cars Applied Engineering

DOE Office of Scientific and Technical Information (OSTI.GOV)

LBNL

2008-07-02

June 30, 2004 Berkeley Lab lecture: Deb Hopkins works with industries like automobile, mining and paper to improve their evaluation and measuring techniques. For several years, she has coordinated ... June 30, 2004 Berkeley Lab lecture: Deb Hopkins works with industries like automobile, mining and paper to improve their evaluation and measuring techniques. For several years, she has coordinated a program at Berkeley Lab funded under the Partnership for a New Generation of Vehicles, a collaboration between the federal government and the U.S. Council for Automotive Research. Nondestructive evaluation techniques to test a car's structural integrity are being developed formore » auto assembly lines.« less

A Window into Longer Lasting Batteries

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2016-11-29

There’s a new tool in the push to engineer rechargeable batteries that last longer and charge more quickly. An X-ray microscopy technique recently developed at Berkeley Lab has given scientists the ability to image nanoscale changes inside lithium-ion battery particles as they charge and discharge. The real-time images provide a new way to learn how batteries work, and how to improve them. The method was developed at Berkeley Lab’s Advanced Light Source, a DOE Office of Science User Facility, by a team of researchers from the Department of Energy’s SLAC National Accelerator Laboratory, Berkeley Lab, Stanford University, and other institutions.

Maria Lucia Ghirardi | NREL

Science.gov Websites

/pathways Education University of California, Berkeley: Ph.D. in Comparative Biochemistry, 1988. Graduate advisor: Dr. Anastasios Melis University of California, Berkeley: M.A. in Comparative Biochemistry, 1983 ); pp. 35192-35209. Wecker, M., Meuser, J., Posewitz, M. and Ghirardi, M. (2011). "Design of a new

Interviews with Exemplary Teachers: Leon F. Litwack.

ERIC Educational Resources Information Center

Rosenzweig, Roy

2001-01-01

Presents an interview with Leon F. Litwack, a professor of U.S. history at the University of California, Berkeley. Covers topics such as what led him to teaching history, his lectures at Berkeley, themes covered in his U.S. history surveys, and his perception of good teaching. (CMK)

Free Speech Advocates at Berkeley.

ERIC Educational Resources Information Center

Watts, William A.; Whittaker, David

1966-01-01

This study compares highly committed members of the Free Speech Movement (FSM) at Berkeley with the student population at large on 3 sociopsychological foci: general biographical data, religious orientation, and rigidity-flexibility. Questionnaires were administered to 172 FSM members selected by chance from the 10 to 1200 who entered and…

Berkeley Bravado: Rochdale Village, University of California, Berkeley

ERIC Educational Resources Information Center

Woodbridge, Sally

1975-01-01

Rochdale Village is a successful example of active student participation in the design and construction of student housing. The apartment complex reflects students' desire for a village of lowrise, wood-shingled buildings, while meeting the University of California's density requirement of 250 units per acre. (JG)

Next Generation Lighting Technologies (LBNL Summer Lecture Series)

ScienceCinema

Siminovittch, Micheal

2018-04-27

For the past several years, Michael Siminovittch, a researcher in the Environmental Energy Technologies Division of Lawrence Berkeley National Laboratory, has worked to package efficient lighting in an easy-to-use and good-looking lamp. His immensely popular "Berkeley Lamp" has redefined how America lights its offices.

Multiple Landslide-Hazard Scenarios Modeled for the Oakland-Berkeley Area, Northern California

USGS Publications Warehouse

Pike, Richard J.; Graymer, Russell W.

2008-01-01

With the exception of Los Angeles, perhaps no urban area in the United States is more at risk from landsliding, triggered by either precipitation or earthquake, than the San Francisco Bay region of northern California. By January each year, seasonal winter storms usually bring moisture levels of San Francisco Bay region hillsides to the point of saturation, after which additional heavy rainfall may induce landslides of various types and levels of severity. In addition, movement at any time along one of several active faults in the area may generate an earthquake large enough to trigger landslides. The danger to life and property rises each year as local populations continue to expand and more hillsides are graded for development of residential housing and its supporting infrastructure. The chapters in the text consist of: *Introduction by Russell W. Graymer *Chapter 1 Rainfall Thresholds for Landslide Activity, San Francisco Bay Region, Northern California by Raymond C. Wilson *Chapter 2 Susceptibility to Deep-Seated Landsliding Modeled for the Oakland-Berkeley Area, Northern California by Richard J. Pike and Steven Sobieszczyk *Chapter 3 Susceptibility to Shallow Landsliding Modeled for the Oakland-Berkeley Area, Northern California by Kevin M. Schmidt and Steven Sobieszczyk *Chapter 4 Landslide Hazard Modeled for the Cities of Oakland, Piedmont, and Berkeley, Northern California, from a M=7.1 Scenario Earthquake on the Hayward Fault Zone by Scott B. Miles and David K. Keefer *Chapter 5 Synthesis of Landslide-Hazard Scenarios Modeled for the Oakland-Berkeley Area, Northern California by Richard J. Pike The plates consist of: *Plate 1 Susceptibility to Deep-Seated Landsliding Modeled for the Oakland-Berkeley Area, Northern California by Richard J. Pike, Russell W. Graymer, Sebastian Roberts, Naomi B. Kalman, and Steven Sobieszczyk *Plate 2 Susceptibility to Shallow Landsliding Modeled for the Oakland-Berkeley Area, Northern California by Kevin M. Schmidt and Steven Sobieszczyk *Plate 3 Susceptibility to Shallow Landsliding Modeled for the Cities of Oakland and Piedmont Northern California by Kevin M. Schmidt and Steven Sobieszczyk *Plate 4 Seismic Landslide Hazard Modeled for the Cities of Oakland, Piedmont, and Berkeley, Northern California by Scott B. Miles and David K. Keefer III The relative hazard for each of several landslide scenarios is presented as a geospatial database. This publication includes ARC/INFO (Environmental Systems Research Institute, http://www.esri.com) version 8.1.2 grids and associated tables and four text files of FGDC-compliant metadata for each grid.

Higher Retail Prices of Sugar-Sweetened Beverages 3 Months After Implementation of an Excise Tax in Berkeley, California

PubMed Central

Rojas, Nadia; Grummon, Anna H.; Madsen, Kristine A.

2015-01-01

Objectives. We assessed the short-term ability to increase retail prices of the first US 1-cent-per-ounce excise tax on the distribution of sugar-sweetened beverages (SSBs), which was implemented in March 2015 by Berkeley, California. Methods. In 2014 and 2015, we examined pre- to posttax price changes of SSBs and non-SSBs in a variety of retailers in Berkeley and in the comparison cities Oakland and San Francisco, California. We examined price changes by beverage, brand, size, and retailer type. Results. For smaller beverages (≤ 33.8 oz), price increases (cents/oz) in Berkeley relative to those in comparison cities were 0.69 (95% confidence interval [CI] = 0.36, 1.03) for soda, 0.47 (95% CI = 0.08, 0.87) for fruit-flavored beverages, and 0.47 (95% CI = 0.25, 0.69) for SSBs overall. For 2-liter bottles and multipacks of soda, relative price increases were 0.46 (95% CI = 0.03, 0.89) and 0.49 (95% CI = 0.21, 0.77). We observed no relative price increases for nontaxed beverages overall. Conclusions. Approximately 3 months after the tax was implemented, SSB retail prices increased more in Berkeley than in nearby cities, marking a step in the causal pathway between the tax and reduced SSB consumption. PMID:26444622

Documentation for a web site to serve ULF-EM (Ultra-Low Frequency Electromagnetic) data to the public

USGS Publications Warehouse

Neumann, Danny A.; McPherson, Selwyn; Klemperer, Simon L.; Glen, Jonathan M.G.; McPhee, Darcy K.; Kappler, Karl

2011-01-01

The Stanford Ultra-Low Frequency Electromagnetic (ULF-EM) Monitoring Project is recording naturally varying electromagnetic signals adjacent to active earthquake faults, in an attempt to establish whether there is any variation in these signals associated with earthquakes. Our project is collaborative between Stanford University, the U.S. Geological Survey (USGS), and UC Berkeley. Lead scientists are Simon Klemperer (Stanford University), Jonathan Glen (USGS) and Darcy Karakelian McPhee (USGS). Our initial sites are in the San Francisco Bay Area, monitoring different strands of the San Andreas fault system, at Stanford University's Jasper Ridge Biological Preserve (JRSC), Marin Headlands of the Golden Gate National Recreation Area (MHDL), and the UC Berkeley's Russell Reservation Field Station adjacent to Briones Regional Park (BRIB). In addition, we maintain in conjunction with the Berkeley Seismological Laboratory (BSL) two remote reference stations at the Bear Valley Ranch in Parkfield, Calif., (PKD) and the San Andreas Geophysical Observatory at Hollister, Calif., (SAO). Metadata about our site can be found at http://ulfem-data.stanford.edu/info.html. Site descriptions can be found at the BSL at http://seismo.berkeley.edu/, and seismic data can be obtained from the Northern California Earthquake Data Center at http://www.ncedc.org/. The site http://ulfem-data.stanford.edu/ allows access to data from the Stanford-USGS sites JRSC, MHDL and BRIB, as well as UC Berkeley sites PKD and SAO.

Higher Retail Prices of Sugar-Sweetened Beverages 3 Months After Implementation of an Excise Tax in Berkeley, California.

PubMed

Falbe, Jennifer; Rojas, Nadia; Grummon, Anna H; Madsen, Kristine A

2015-11-01

We assessed the short-term ability to increase retail prices of the first US 1-cent-per-ounce excise tax on the distribution of sugar-sweetened beverages (SSBs), which was implemented in March 2015 by Berkeley, California. In 2014 and 2015, we examined pre- to posttax price changes of SSBs and non-SSBs in a variety of retailers in Berkeley and in the comparison cities Oakland and San Francisco, California. We examined price changes by beverage, brand, size, and retailer type. For smaller beverages (≤ 33.8 oz), price increases (cents/oz) in Berkeley relative to those in comparison cities were 0.69 (95% confidence interval [CI] = 0.36, 1.03) for soda, 0.47 (95% CI = 0.08, 0.87) for fruit-flavored beverages, and 0.47 (95% CI = 0.25, 0.69) for SSBs overall. For 2-liter bottles and multipacks of soda, relative price increases were 0.46 (95% CI = 0.03, 0.89) and 0.49 (95% CI = 0.21, 0.77). We observed no relative price increases for nontaxed beverages overall. Approximately 3 months after the tax was implemented, SSB retail prices increased more in Berkeley than in nearby cities, marking a step in the causal pathway between the tax and reduced SSB consumption.

NCRVE Change Agent. Shaping the Future of Vocational Education. Volume 4, 1994.

ERIC Educational Resources Information Center

NCRVE Change Agent, 1994

1994-01-01

The first of four issues in this volume consists of four review articles. "Tech Prep Quality" reviews a guide to total quality management and tech prep. "Specific, Ocean to Ocean" reviews a publication that identifies 10 preliminary plans for implementing tech prep. "...No Single Option" is about a monograph that evaluates tech prep and other…

Selected Outcomes Related to Tech Prep Implementation by Illinois Consortia, 2001-2005

ERIC Educational Resources Information Center

Bragg, Debra D.; Kirby, Catherine; Zhu, Rongchun

2006-01-01

This report is the summary of key aspects of Tech Prep in Illinois over the five year period of 2001-2005 during which all Tech Prep consortia provided annual data based on federal legislative requirements and state-determined essential elements of successful programs. These annual Tech Prep reports enable local educators to monitor student…

Alumni Giving at Arkansas Tech University: College Experiences and Motivations to Give as Predictors of Giving Behavior

ERIC Educational Resources Information Center

Lackie, Mary Bane

2010-01-01

The purpose of this study was to examine the characteristics of the "Lost Generation" alumni who ended their attendance or graduated from Arkansas Tech University (Tech) between January 1973 and December 1995 relating to their demographic characteristics, perceptions of college experiences, involvement with Tech after graduation, and attitudes…

77 FR 26588 - In the Matter of Recycle Tech, Inc.; Order of Suspension of Trading

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-04

... SECURITIES AND EXCHANGE COMMISSION [File No. 500-1] In the Matter of Recycle Tech, Inc.; Order of Suspension of Trading May 2, 2012. It appears to the Securities and Exchange Commission that there is a lack of current and accurate information concerning the securities of Recycle Tech, Inc. (``Recycle Tech...

Cost Reduction Through the Use of Additive Manufacturing (3d Printing) and Collaborative Product Life Cycle Management Technologies to Enhance the Navy’s Maintenance Programs

DTIC Science & Technology

2013-09-01

the order from the DLA; convenes a meeting with tech librarians , engineers, machinists, quality assurance (QA) inspectors, and mechanics to assess...created, begins the in-house process. 3. Research of Technical Drawings The tech librarian reviews the applicable repository for any tech drawings...applicable to Widget A. If none are found, the tech librarian contacts the OEM and other D-Level activities to find out whether the tech drawing is out

Berkeley Lab Wins Seven 2015 R&D 100 Awards | Berkeley Lab

Science.gov Websites

products from industry, academia, and government-sponsored research, ranging from chemistry to materials to problems in metrology techniques: the quantitative characterization of the imaging instrumentation Computational Research Division led the development of the technology. Sensor Integrated with Recombinant and

PhyloChip Tackles Coral Disease

ScienceCinema

DeSantis, Todd

2017-12-13

Scientists at Berkeley Lab and the University of California, Merced are using an innovative DNA array developed at Berkeley Lab to catalog the microbes that live among coral in the tropical waters off the coast of Puerto Rico. More info: http://newscenter.lbl.gov/feature-stories/2009/02/02/coral-reefs/

Teaching at Berkeley: A Guide for Foreign Teaching Assistants.

ERIC Educational Resources Information Center

Cohen, Robby, Ed.; Robin, Ron, Ed.

A handbook for foreign teaching assistants (TAs) is presented by foreign graduate students with teaching experience and other educators who have worked closely with them. Language skills, teaching strategies, cultural issues, resources, and the environment at the University of California, Berkeley, are addressed in 16 articles. Article titles and…

Improved Cosmological Constraints from New, Old, and Combined Supernova

Science.gov Websites

Data Set SAO/NASA ADS Astronomy Abstract Service Title: Improved Cosmological Constraints from , Harvard University, 60 Garden Street, Cambridge, MA 02138), AK(Department of Astronomy and Astrophysics Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720), BI(Department of Physics and Astronomy

Computerized Serial Processing System at the University of California, Berkeley

ERIC Educational Resources Information Center

Silberstein, Stephen M.

1975-01-01

The extreme flexibility of the MARC format coupled with the simplicity of a batch-oriented processing system centered around a sequential master file has enabled the University of California, Berkeley, library to gradually build an unusually large serials data base in support of both technical and public services. (Author)

The Universe Adventure - Help

Science.gov Websites

teachers introduce the material to their students. Clicking the link will open another window with activity ideas or worksheets to download. The number of stars next to the activity name indicates how difficult . Bechtel, Jr. Foundation [ Site Map ] optimized for Firefox [ UC Berkeley ] [ UC Berkeley Physics

The Berkeley Environmental Simulation Laboratory: Its Use In Environmental Impact Assessment.

ERIC Educational Resources Information Center

Appleyard, Donald; And Others

An environmental simulation laboratory at the University of California, Berkeley, is testing the adequacy of different techniques for simulating environmental experiences. Various levels of realism, with various costs, are available in different presentation modes. The simulations can aid in communication about and the resolution of environmental…

The Ph.D. Production Function: The Case at Berkeley.

ERIC Educational Resources Information Center

Breneman, David W.

This report analyzes departmental variations in time to degree and attrition in Ph.D. programs at Berkeley. An alternative hypothesis, the Ph.D. production function, is examined by cross-section econometric analysis of 28 departments. The inputs included in the production function were student variables--quality and percent males; faculty…

The structure of a cholesterol-trapping protein

Science.gov Websites

Date February 28, 2003 Date Berkeley Lab Science Beat Berkeley Lab Science Beat The structure of a Institute researchers determined the three-dimensional structure of a protein that controls cholesterol level in the bloodstream. Knowing the structure of the protein, a cellular receptor that ensnares

Smart Energy Management of Multiple Full Cell Powered Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

MOhammad S. Alam

2007-04-23

In this research project the University of South Alabama research team has been investigating smart energy management and control of multiple fuel cell power sources when subjected to varying demands of electrical and thermal loads together with demands of hydrogen production. This research has focused on finding the optimal schedule of the multiple fuel cell power plants in terms of electric, thermal and hydrogen energy. The optimal schedule is expected to yield the lowest operating cost. Our team is also investigating the possibility of generating hydrogen using photoelectrochemical (PEC) solar cells through finding materials for efficient light harvesting photoanodes. Themore » goal is to develop an efficient and cost effective PEC solar cell system for direct electrolysis of water. In addition, models for hydrogen production, purification, and storage will be developed. The results obtained and the data collected will be then used to develop a smart energy management algorithm whose function is to maximize energy conservation within a managed set of appliances, thereby lowering O/M costs of the Fuel Cell power plant (FCPP), and allowing more hydrogen generation opportunities. The Smart Energy Management and Control (SEMaC) software, developed earlier, controls electrical loads in an individual home to achieve load management objectives such that the total power consumption of a typical residential home remains below the available power generated from a fuel cell. In this project, the research team will leverage the SEMaC algorithm developed earlier to create a neighborhood level control system.« less

Mutational analysis of genes coding for cell surface proteins in colorectal cancer cell lines reveal novel altered pathways, druggable mutations and mutated epitopes for targeted therapy

PubMed Central

Correa, Bruna R.; Bettoni, Fabiana; Koyama, Fernanda C.; Navarro, Fabio C.P.; Perez, Rodrigo O.; Mariadason, John; Sieber, Oliver M.; Strausberg, Robert L.; Simpson, Andrew J.G.; Jardim, Denis L.F.; Reis, Luiz Fernando L.; Parmigiani, Raphael B.; Galante, Pedro A.F.; Camargo, Anamaria A.

2014-01-01

We carried out a mutational analysis of 3,594 genes coding for cell surface proteins (Surfaceome) in 23 colorectal cancer cell lines, searching for new altered pathways, druggable mutations and mutated epitopes for targeted therapy in colorectal cancer. A total of 3,944 somatic non-synonymous substitutions and 595 InDels, occurring in 2,061 (57%) Surfaceome genes were catalogued. We identified 48 genes not previously described as mutated in colorectal tumors in the TCGA database, including genes that are mutated and expressed in >10% of the cell lines (SEMA4C, FGFRL1, PKD1, FAM38A, WDR81, TMEM136, SLC36A1, SLC26A6, IGFLR1). Analysis of these genes uncovered important roles for FGF and SEMA4 signaling in colorectal cancer with possible therapeutic implications. We also found that cell lines express on average 11 druggable mutations, including frequent mutations (>20%) in the receptor tyrosine kinases AXL and EPHA2, which have not been previously considered as potential targets for colorectal cancer. Finally, we identified 82 cell surface mutated epitopes, however expression of only 30% of these epitopes was detected in our cell lines. Notwithstanding, 92% of these epitopes were expressed in cell lines with the mutator phenotype, opening new venues for the use of “general” immune checkpoint drugs in this subset of patients. PMID:25193853

Extensive epistasis for olfactory behaviour, sleep and waking activity in Drosophila melanogaster.

PubMed

Swarup, Shilpa; Harbison, Susan T; Hahn, Lauren E; Morozova, Tatiana V; Yamamoto, Akihiko; Mackay, Trudy F C; Anholt, Robert R H

2012-02-01

Epistasis is an important feature of the genetic architecture of quantitative traits, but the dynamics of epistatic interactions in natural populations and the relationship between epistasis and pleiotropy remain poorly understood. Here, we studied the effects of epistatic modifiers that segregate in a wild-derived Drosophila melanogaster population on the mutational effects of P-element insertions in Semaphorin-5C (Sema-5c) and Calreticulin (Crc), pleiotropic genes that affect olfactory behaviour and startle behaviour and, in the case of Crc, sleep phenotypes. We introduced Canton-S B (CSB) third chromosomes with or without a P-element insertion at the Crc or Sema-5c locus in multiple wild-derived inbred lines of the Drosophila melanogaster Genetic Reference Panel (DGRP) and assessed the effects of epistasis on the olfactory response to benzaldehyde and, for Crc, also on sleep. In each case, we found substantial epistasis and significant variation in the magnitude of epistasis. The predominant direction of epistatic effects was to suppress the mutant phenotype. These observations support a previous study on startle behaviour using the same D. melanogaster chromosome substitution lines, which concluded that suppressing epistasis may buffer the effects of new mutations. However, epistatic effects are not correlated among the different phenotypes. Thus, suppressing epistasis appears to be a pervasive general feature of natural populations to protect against the effects of new mutations, but different epistatic interactions modulate different phenotypes affected by mutations at the same pleiotropic gene.

A whole genome Bayesian scan for adaptive genetic divergence in West African cattle

PubMed Central

2009-01-01

Background The recent settlement of cattle in West Africa after several waves of migration from remote centres of domestication has imposed dramatic changes in their environmental conditions, in particular through exposure to new pathogens. West African cattle populations thus represent an appealing model to unravel the genome response to adaptation to tropical conditions. The purpose of this study was to identify footprints of adaptive selection at the whole genome level in a newly collected data set comprising 36,320 SNPs genotyped in 9 West African cattle populations. Results After a detailed analysis of population structure, we performed a scan for SNP differentiation via a previously proposed Bayesian procedure including extensions to improve the detection of loci under selection. Based on these results we identified 53 genomic regions and 42 strong candidate genes. Their physiological functions were mainly related to immune response (MHC region which was found under strong balancing selection, CD79A, CXCR4, DLK1, RFX3, SEMA4A, TICAM1 and TRIM21), nervous system (NEUROD6, OLFM2, MAGI1, SEMA4A and HTR4) and skin and hair properties (EDNRB, TRSP1 and KRTAP8-1). Conclusion The main possible underlying selective pressures may be related to climatic conditions but also to the host response to pathogens such as Trypanosoma(sp). Overall, these results might open the way towards the identification of important variants involved in adaptation to tropical conditions and in particular to resistance to tropical infectious diseases. PMID:19930592

Disability Evaluation System Analysis and Research Annual Report 2017

DTIC Science & Technology

2017-11-20

Amanda L. Kelley, MPH Program Manager, AMSARA Deputy Program Manager, AMSARA Contractor, ManTech Health Contractor, ManTech Health Christine...Toolin, MS Cordie K. Campbell, MPH Public Health Analyst, AMSARA Public Health Analyst, AMSARA Contractor, ManTech Health Contractor...ManTech Health Preventive Medicine Branch Walter Reed Army Institute of Research 503 Robert Grant Road, Forest Glen Annex Silver

TechEdSat Nano-Satellite Series Fact Sheet

NASA Technical Reports Server (NTRS)

Murbach, Marcus; Martinez, Andres; Guarneros Luna, Ali

2014-01-01

TechEdSat-3p is the second generation in the TechEdSat-X series. The TechEdSat Series uses the CubeSat standards established by the California Polytechnic State University Cal Poly), San Luis Obispo. With typical blocks being constructed from 1-unit (1U 10x10x10 cm) increments, the TechEdSat-3p has a 3U volume with a 30 cm length. The project uniquely pairs advanced university students with NASA researchers in a rapid design-to-flight experience lasting 1-2 semesters.The TechEdSat Nano-Satellite Series provides a rapid platform for testing technologies for future NASA Earth and planetary missions, as well as providing students with an early exposure to flight hardware development and management.

Accessing northern California earthquake data via Internet

NASA Astrophysics Data System (ADS)

Romanowicz, Barbara; Neuhauser, Douglas; Bogaert, Barbara; Oppenheimer, David

The Northern California Earthquake Data Center (NCEDC) provides easy access to central and northern California digital earthquake data. It is located at the University of California, Berkeley, and is operated jointly with the U.S. Geological Survey (USGS) in Menlo Park, Calif., and funded by the University of California and the National Earthquake Hazard Reduction Program. It has been accessible to users in the scientific community through Internet since mid-1992.The data center provides an on-line archive for parametric and waveform data from two regional networks: the Northern California Seismic Network (NCSN) operated by the USGS and the Berkeley Digital Seismic Network (BDSN) operated by the Seismographic Station at the University of California, Berkeley.

Fracture trace map and single-well aquifer test results in a carbonate aquifer in Berkeley County, West Virginia

USGS Publications Warehouse

McCoy, Kurt J.; Podwysocki, Melvin H.; Crider, E. Allen; Weary, David J.

2005-01-01

These data contain information on the results of single-well aquifer tests, lineament analysis, and a bedrock geologic map compilation for the low-lying carbonate and shale areas of eastern Berkeley County, West Virginia. Efforts have been initiated by management agencies of Berkeley County in cooperation with the U.S. Geological Survey to further the understanding of the spatial distribution of fractures in the carbonate regions and their correlation with aquifer properties. This report presents transmissivity values from about 200 single-well aquifer tests and a map of fracture-traces determined from aerial photos and field investigations. Transmissivity values were compared to geologic factors possibly affecting its magnitude.

A community of scientists: cultivating scientific identity among undergraduates within the Berkeley Compass Project

NASA Astrophysics Data System (ADS)

Aceves, Ana V.; Berkeley Compass Project

2015-01-01

The Berkeley Compass Project is a self-formed group of graduate and undergraduate students in the physical sciences at UC Berkeley. Our goals are to improve undergraduate physics education, provide opportunities for professional development, and increase retention of students from populations typically underrepresented in the physical sciences. For students who enter as freshmen, the core Compass experience consists of a summer program and several seminar courses. These programs are designed to foster a diverse, collaborative student community in which students engage in authentic research practices and regular self-reflection. Compass encourages undergraduates to develop an identity as a scientist from the beginning of their university experience.

34 CFR 406.31 - How does a State carry out the State-Administered Tech-Prep Education Program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 3 2011-07-01 2011-07-01 false How does a State carry out the State-Administered Tech... TECH-PREP EDUCATION PROGRAM What Conditions Must Be Met After a State Receives an Award? § 406.31 How does a State carry out the State-Administered Tech-Prep Education Program? (a) A State board carries...

Evidence-Based Design for Project-Based Learning: A Case Study for a 50,000 SF Addition Dedicated to the New Tech Curriculum

ERIC Educational Resources Information Center

Moretti, Richard D.; Conte, Philip R.

2012-01-01

The Seaford School District, Seaford, Delaware, determined that a component of their "reinvention" of Seaford High School would be the creation of a New Tech Academy, affiliated with the New Tech Network and housed in an addition to that building. The New Tech Network, headquartered in Napa, California, is a rapidly growing association…

Frontiers in Laser Cooling, Single-Molecule Biophysics, and Energy Science: A Talk from Eric Cornell

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cornell, Eric

2008-08-30

Eric Cornell presents a talk at Frontiers in Laser Cooling, Single-Molecule Biophysics and Energy Science, a scientific symposium honoring Steve Chu, director of Lawrence Berkeley National Laboratory and recipient of the 1997 Nobel Prize in Physics. The symposium was held August 30, 2008 in Berkeley.

Frontiers in Laser Cooling, Single-Molecule Biophysics, and Energy Science: A Talk from Kurt Gibble

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gibble, Kurt

2008-08-30

Kurt Gibble presents a talk at Frontiers in Laser Cooling, Single-Molecule Biophysics and Energy Science, a scientific symposium honoring Steve Chu, director of Lawrence Berkeley National Laboratory and recipient of the 1997 Nobel Prize in Physics. The symposium was held August 30, 2008 in Berkeley.

Frontiers in Laser Cooling, Single-Molecule Biophysics, and Energy Science: A Talk from Jay Keasling

DOE Office of Scientific and Technical Information (OSTI.GOV)

Keasling, Jay

2008-08-30

Jay Keasling presents a talk at Frontiers in Laser Cooling, Single-Molecule Biophysics and Energy Science, a scientific symposium honoring Steve Chu, director of Lawrence Berkeley National Laboratory and recipient of the 1997 Nobel Prize in Physics. The symposium was held August 30, 2008 in Berkeley.

Frontiers in Laser Cooling, Single-Molecule Biophysics, and Enrgy Science: A Talk by Carl Wieman

ScienceCinema

Wieman, Carl

2017-12-09

Carl Wieman presents a talk at Frontiers in Laser Cooling, Single-Molecule Biophysics and Energy Science, a scientific symposium honoring Steve Chu, director of Lawrence Berkeley National Laboratory and recipient of the 1997 Nobel Prize in Physics. The symposium was held August 30, 2008 in Berkeley.

Berkeley Lab - Materials Sciences Division

Science.gov Websites

; Finance Templates Travel One-Stop Personnel Resources Committees In Case of Emergency Looking for MSD0010 Officer Mary Gross MCGross@lbl.gov Research Group Representatives Group Rep Ager Rachel Woods-Robinson Somorjai (see Salmeron Group) Yaghi Xiaokun Pei xiaokun_pei@berkeley.edu Zhang Sui Yang SuiYang@lbl.gov

Carbon Flux Explorers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bishop, Jim

Jim Bishop, senior scientist at Berkeley Lab and professor at UC Berkeley, is leading a project to deploy robotic floats that provide data on how microorganisms sequester carbon in the ocean. He recently led a research team on a 10-day voyage, funded by the National Science Foundation, to put the Carbon Flux Explorers to the test.

Care for Our Children: A Comprehensive Plan for Child Care Services in Berkeley.

ERIC Educational Resources Information Center

Pacific Training and Technical Assistance Corp., Berkeley, CA.

This document reports research and recommendations made by the Pacific Training and Technical Assistance Corporation for a comprehensive child-care program in Berkeley. The report is divided into two sections. Section I, "Research and Planning," describes research methodology and findings and includes demographic information on the city…

Frontiers in Laser Cooling, Single-Molecule Biophysics, and Energy Science: A Talk from Eric Cornell

ScienceCinema

Cornell, Eric

2018-02-05

Eric Cornell presents a talk at Frontiers in Laser Cooling, Single-Molecule Biophysics and Energy Science, a scientific symposium honoring Steve Chu, director of Lawrence Berkeley National Laboratory and recipient of the 1997 Nobel Prize in Physics. The symposium was held August 30, 2008 in Berkeley.

Neighborhood organization activities: evacuation drills, clusters, and fire safety awareness

Treesearch

Dick White

1995-01-01

Emergency preparedness activities of one Berkeley-Oakland Hills neighborhood at the wildland/urban interface include establishing clusters that reduce fire hazards and fuel loads, setting aside emergency supplies, and identifying evacuation routes; taking emergency preparedness courses from the Offices of Emergency Services of Berkeley and Oakland (the CERT and CORE...

Ting Xu

Science.gov Websites

California, Berkeley tingxu@berkeley.edu 510-642-1632 Research profile » A U.S. Department of Energy National Laboratory Operated by the University of California UC logo Questions & Comments * Privacy Computational Study of Excited-State Phenomena in Energy Materials Center for X-ray Optics MSD Facilities Ion

Electron Microscope Center Opens at Berkeley.

ERIC Educational Resources Information Center

Robinson, Arthur L.

1981-01-01

A 1.5-MeV High Voltage Electron Microscope has been installed at the Lawrence Berkeley Laboratory which will help materials scientists and biologists study samples in more true-to-life situations. A 1-MeV Atomic Resolution Microscope will be installed at the same location in two years which will allow scientists to distinguish atoms. (DS)

Energy Efficient Buildings and Appliances: From Berkeley Lab to the Marketplace (LBNL Summer Lecture Series)

ScienceCinema

Rosenfeld, Art [California Energy Commission, Sacramento, CA (United States)

2018-02-16

Summer Lecture Series 2006: Art Rosenfeld, an appointee to the California Energy Commission and one of the architects of energy efficiency research at Berkeley Lab in the 1970s, discusses what it takes to shepherd innovative energy efficiency research from the lab to the real world.

Frontiers in Laser Cooling, Single-Molecule Biophysics, and Energy Science: A Talk from Jay Keasling

ScienceCinema

Keasling, Jay

2018-02-14

Jay Keasling presents a talk at Frontiers in Laser Cooling, Single-Molecule Biophysics and Energy Science, a scientific symposium honoring Steve Chu, director of Lawrence Berkeley National Laboratory and recipient of the 1997 Nobel Prize in Physics. The symposium was held August 30, 2008 in Berkeley.

A CAT scan for cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2009-01-01

Recently, a team of scientists from Berkeley Lab, Stanford University, and the University of California, San Francisco used Berkeley Lab's National Center for X-ray Tomography to capture the changes that occur when Candida albicans is exposed to a new and promising antifungal therapy. http://newscenter.lbl.gov/feature-stories/2009/12/10/cat-scan-cells/

Carbon Smackdown: Cookstoves for the developing world

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ashok Gadgil, Kayje Booker, and Adam Rausch

2010-07-07

In this June 30, 2010 Berkeley Lab summer lecture, learn how efficient cookstoves for the developing world — from Darfur to Ethiopia and beyond — are reducing carbon dioxide emissions, saving forests, and improving health. Berkeley Lab's Ashok Gadgil, Kayje Booker, and Adam Rausch discuss why they got started in this great challenge and what's next.

Frontiers in Laser Cooling, Single-Molecule Biophysics, and Enrgy Science: A Talk by Carl Wieman

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wieman, Carl

Carl Wieman presents a talk at Frontiers in Laser Cooling, Single-Molecule Biophysics and Energy Science, a scientific symposium honoring Steve Chu, director of Lawrence Berkeley National Laboratory and recipient of the 1997 Nobel Prize in Physics. The symposium was held August 30, 2008 in Berkeley.

In Search of Good Governance

ERIC Educational Resources Information Center

Grush, Mary

2007-01-01

Since his retirement in 2005, UC-Berkeley's Associate Vice Chancellor and CIO Emeritus and current ECAR fellow John (Jack) McCredie has devoted much of his professional energy to studying and writing/speaking about IT governance and leadership in higher education. In his role at UC-Berkeley, he was responsible for leading central IT support for…

Carbon Flux Explorers

ScienceCinema

Bishop, Jim

2018-06-12

Jim Bishop, senior scientist at Berkeley Lab and professor at UC Berkeley, is leading a project to deploy robotic floats that provide data on how microorganisms sequester carbon in the ocean. He recently led a research team on a 10-day voyage, funded by the National Science Foundation, to put the Carbon Flux Explorers to the test.

Undergraduate Research Participation at the University of California, Berkeley. Research & Occasional Paper Series: CSHE.17.08

ERIC Educational Resources Information Center

Berkes, Elizabeth

2008-01-01

Although the University of California, Berkeley has increased efforts to involve undergraduates in scientific research, little data exists regarding the number of undergraduate researchers. The University of California Undergraduate Experience Survey (UCUES) presents an opportunity to investigate the extent of undergraduate research involvement at…

The Berkeley Puppet Interview: A Screening Instrument for Measuring Psychopathology in Young Children

ERIC Educational Resources Information Center

Stone, Lisanne L.; van Daal, Carlijn; van der Maten, Marloes; Engels, Rutger C. M. E.; Janssens, Jan M. A. M.; Otten, Roy

2014-01-01

Background: While child self-reports of psychopathology are increasingly accepted, little standardized instruments are utilized for these practices. The Berkeley Puppet Interview (BPI) is an age-appropriate instrument for self-reports of problem behavior by young children. Objective: Psychometric properties of the Dutch version of the BPI will be…

"A Woman's World": The University of California, Berkeley, during the Second World War

ERIC Educational Resources Information Center

Dorn, Charles

2008-01-01

During World War II, female students at the University of California, Berkeley--then the most populous undergraduate campus in American higher education--made significant advances in collegiate life. In growing numbers, women enrolled in male-dominated academic programs, including mathematics, chemistry, and engineering, as they prepared for…

Frontiers in Laser Cooling, Single-Molecule Biophysics, and Energy Science: A Talk from Kurt Gibble

ScienceCinema

Gibble, Kurt

2018-02-05

Kurt Gibble presents a talk at Frontiers in Laser Cooling, Single-Molecule Biophysics and Energy Science, a scientific symposium honoring Steve Chu, director of Lawrence Berkeley National Laboratory and recipient of the 1997 Nobel Prize in Physics. The symposium was held August 30, 2008 in Berkeley.

Lawrence Berkeley National Laboratory 2015 Annual Financial Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Williams, Kim, P

FY2015 financial results reflect a year of significant scientific, operational and financial achievement for Lawrence Berkeley National Laboratory. Complementing many scientific accomplishments, Berkeley Lab completed construction of four new research facilities: the General Purpose Laboratory, Chu Hall, Wang Hall and the Flexlab Building Efficiency Testbed. These state-of-the-art facilities allow for program growth and enhanced collaboration, in part by enabling programs to return to the Lab’s Hill Campus from offsite locations. Detailed planning began for the new Integrative Genomics Building (IGB) that will house another major program currently located offsite. Existing site infrastructure was another key focus area. The Lab prioritizedmore » and increased investments in deferred maintenance in alignment with the Berkeley Lab Infrastructure Plan, which was developed under the leadership of the DOE Office of Science. With the expiration of American Recovery and Reinvestment Act (ARRA) funds, we completed the close-out of all of our 134 ARRA projects, recording total costs of $331M over the FY2009-2015 period. Download the report to read more.« less

A Radiation Homeland Security Workshop Presented to the City of Berkeley Fire Department

NASA Astrophysics Data System (ADS)

Matis, Howard

2005-04-01

A radiation incident in a community, ranging from a transportation accident to a dirty bomb, is expected to be rare, but still can occur. First responders to such an incident must be prepared. City of Berkeley officials met with members of the Lawrence Berkeley National Laboratory staff and agreed that the laboratory participants would create material and teach it to all of their fire fighting staff. To design such a course, nuclear physicists, biologists and health physicists merged some of their existing teaching material together with previous homeland security efforts to produce a course that lasted one full day. The material was designed to help alleviate the myths and fear of radiation experienced by many first responders. It included basic nuclear physics information, biological effects, and methods that health physicists use to detect and handle radiation. The curriculum included several hands on activities which involved working directly with the meters the Berkeley Fire Department possessed. In addition, I will discuss some observations from teaching this course material plus some unusual problems that we encountered, such as suddenly the whole class responding to a fire.

Site Environmental Report for 2002, Volume 2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pauer, Ron

2003-07-01

Each year, Ernest Orlando Lawrence Berkeley National Laboratory prepares an integrated report on its environmental programs to satisfy the requirements of United States Department of Energy Order 231.1. The ''Site Environmental Report for 2002'' summarizes Berkeley Lab's compliance with environmental standards and requirements, characterizes environmental management efforts through surveillance and monitoring activities, and highlights significant programs and efforts for calendar year 2002. Throughout this report, Ernest Orlando Lawrence Berkeley National Laboratory is referred to as ''Berkeley Lab,'' ''the Laboratory,'' ''Lawrence Berkeley National Laboratory,'' and ''LBNL.'' The report is separated into two volumes. Volume I contains a general overview of themore » Laboratory, the status of environmental programs, and summarized results from surveillance and monitoring activities. Volume II contains individual data results from the monitoring programs. This year, the ''Site Environmental Report'' was distributed on a CD in PDF format that includes Volume I, Volume II, and related documents. The report is also available on the Web at http://www.lbl.gov/ehs/esg/. The report follows the Laboratory's policy of using the International System of Units (SI), also known as the metric system of measurements. Whenever possible, results are additionally reported using the more conventional (non-SI) system of measurements because this system is referenced by some current regulatory standards and is more familiar to some readers. The tables included at the end of the Glossary are intended to help readers understand the various prefixes used with SI units of measurement and convert these units from one system to the other.« less

Site Environmental Report for 2002, Volume 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pauer, Ron

2003-07-01

Each year, Ernest Orlando Lawrence Berkeley National Laboratory prepares an integrated report on its environmental programs to satisfy the requirements of United States Department of Energy Order 231.1. The ''Site Environmental Report for 2002'' summarizes Berkeley Lab's compliance with environmental standards and requirements, characterizes environmental management efforts through surveillance and monitoring activities, and highlights significant programs and efforts for calendar year 2002. Throughout this report, Ernest Orlando Lawrence Berkeley National Laboratory is referred to as ''Berkeley Lab,'' ''the Laboratory,'' ''Lawrence Berkeley National Laboratory,'' and ''LBNL.'' The report is separated into two volumes. Volume I contains a general overview of themore » Laboratory, the status of environmental programs, and summarized results from surveillance and monitoring activities. Volume II contains individual data results from the monitoring programs. This year, the ''Site Environmental Report'' was distributed on a CD in PDF format that includes Volume I, Volume II, and related documents. The report is also available on the Web at http://www.lbl.gov/ehs/esg/. The report follows the Laboratory's policy of using the International System of Units (SI), also known as the metric system of measurements. Whenever possible, results are additionally reported using the more conventional (non-SI) system of measurements because this system is referenced by some current regulatory standards and is more familiar to some readers. The tables included at the end of the Glossary are intended to help readers understand the various prefixes used with SI units of measurement and convert these units from one system to the other.« less

Site Environmental Report for 2005 Volume I and Volume II

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ruggieri, Michael

2006-07-07

Each year, Ernest Orlando Lawrence Berkeley National Laboratory prepares an integrated report on its environmental programs to satisfy the requirements of United States Department of Energy Order 231.1A, ''Environment, Safety, and Health Reporting''. The ''Site Environmental Report for 2005'' summarizes Berkeley Lab's environmental management performance, presents environmental monitoring results, and describes significant programs for calendar year 2005. (Throughout this report, Ernest Orlando Lawrence Berkeley National Laboratory is referred to as ''Berkeley Lab'', ''the Laboratory'', ''Lawrence Berkeley National Laboratory'', and ''LBNL''.) The report is separated into two volumes. Volume I contains an overview of the Laboratory, the status of environmental programs,more » and summarized results from surveillance and monitoring activities. This year's Volume I text body is organized into an executive summary followed by six chapters. The report's structure has been reorganized this year, and it now includes a chapter devoted to environmental management system topics. Volume II contains individual data results from surveillance and monitoring activities. The ''Site Environmental Report'' is distributed by releasing it on the Web from the Berkeley Lab Environmental Services Group (ESG) home page, which is located at http://www.lbl.gov/ehs/esg/. Many of the documents cited in this report also are accessible from the ESG Web page. CD and printed copies of this Site Environmental Report are available upon request. The report follows the Laboratory's policy of using the International System of Units (SI), also known as the metric system of measurements. Whenever possible, results are also reported using the more conventional (non-SI) system of measurements, because the non-SI system is referenced by several current regulatory standards and is more familiar to some readers. Two tables are provided at the end of the Glossary to help readers: the first defines the prefixes used with SI units of measurement, and the second provides conversions to non-SI units.« less

Adapted managerial mathematical model to study the functions and interactions between enterprises in high-tech cluster

NASA Astrophysics Data System (ADS)

Anguelov, Kiril P.; Kaynakchieva, Vesela G.

2017-12-01

The aim of the current study is to research and analyze Adapted managerial mathematical model to study the functions and interactions between enterprises in high-tech cluster, and his approbation in given high-tech cluster; to create high-tech cluster, taking into account the impact of relationships between individual units in the cluster-Leading Enterprises, network of Enterprises subcontractors, economic infrastructure.

Communications for Lifelong Success I and II (Tech Prep English I and II). Pilot Test Materials for Pendleton High School, Pendleton, South Carolina. Draft.

ERIC Educational Resources Information Center

Turlington, Anita; And Others

These instructional materials include six supplementary units for Tech Prep English I (grade 9) and six assignments for Tech Prep English II (grade 10). The Tech Prep English I units have these components: unit objectives, introduction, and follow-up exercises. Topics include goal setting, problem solving and decision making, listening and reading…

Pharmacist and Technician Perceptions of Tech-Check-Tech in Community Pharmacy Practice Settings.

PubMed

Frost, Timothy P; Adams, Alex J

2018-04-01

Tech-check-tech (TCT) is a practice model in which pharmacy technicians with advanced training can perform final verification of prescriptions that have been previously reviewed for appropriateness by a pharmacist. Few states have adopted TCT in part because of the common view that this model is controversial among members of the profession. This article aims to summarize the existing research on pharmacist and technician perceptions of community pharmacy-based TCT. A literature review was conducted using MEDLINE (January 1990 to August 2016) and Google Scholar (January 1990 to August 2016) using the terms "tech* and check," "tech-check-tech," "checking technician," and "accuracy checking tech*." Of the 7 studies identified we found general agreement among both pharmacists and technicians that TCT in community pharmacy settings can be safely performed. This agreement persisted in studies of theoretical TCT models and in studies assessing participants in actual community-based TCT models. Pharmacists who had previously worked with a checking technician were generally more favorable toward TCT. Both pharmacists and technicians in community pharmacy settings generally perceived TCT to be safe, in both theoretical surveys and in surveys following actual TCT demonstration projects. These perceptions of safety align well with the actual outcomes achieved from community pharmacy TCT studies.

The Coast Artillery Journal. Volume 56, Number 6, June 1922

DTIC Science & Technology

1922-06-01

lay-June, 1922. Le fonctionnement des services a l’interieur de la D. A. (IV).-Be-4, ::\\lay, 1922. Ligeros Apuntes Sobre la Instruccion de Planas...34 which is contained in paragraph 101 to 105,both inclusive . As the items we have considered so far would not occupy a half- hour’s drill period let us...two-arm SemaPhore Code. 1 Letters A-G inclusive . Note that in this course the let- 2 A-H, Z. ters are grouped as nearly as 3 A-I, X, Z. possible with

Get on Board the Cost Effective Way: A Tech Prep Replication Process.

ERIC Educational Resources Information Center

Moore, Wayne A.; Szul, Linda F.; Rivosecchi, Karen

1997-01-01

The Northwestern Pennsylvania Tech Prep Consortium model for replicating tech prep programs includes these steps: fact finding, local industry analysis, curriculum development, detailed description, marketing strategies, implementation, and program evaluation. (SK)

Toward healthy prisons: the TECH model and its applications.

PubMed

Ross, Michael W; Jo Harzke, Amy

2012-01-01

This paper aims to explore how the TECH Model (testing for and treating infectious diseases and vaccination; environmental modification to prevent disease transmission; chronic disease identification and treatment; and health maintenance and education) can be used for assessing and achieving healthy prisons. This paper explores the concepts of "health in prison" and "healthy prisons" in the context of recent research and guidance. The paper then considers the TECH Model as an approach to achieving healthy prisons. Under each of the four TECH Model domains are tasks to achieve a healthy prison. For prisons with poor or no resources, each domain contains steps that will improve prison health and move towards a healthy prison for both prisoners and staff. Implementation can thus be "low-TECH" or "high-TECH" depending on the setting and the available resources and the model is specifically designed to provide options for resource-poor as well as resource-rich correctional settings. The TECH Model is a first step in characterizing the components of a healthy prison and the processes to achieve this. This Model could be implemented in all levels of prisons internationally.

The ORNL Chemical Technology Division, 1950-1994

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jolley, R.L.; Genung, R.K.; McNeese, L.E.

1994-10-01

This document attempts to reconstruct the role played by the Chemical Technology Division (Chem Tech) of the Oak Ridge National Laboratory (ORNL) in the atomic era since the 1940`s related to the development and production of nuclear weapons and power reactors. Chem Tech`s early contributions were landmark pioneering studies. Unknown and dimly perceived problems like chemical hazards, radioactivity, and criticality had to be dealt with. New chemical concepts and processes had to be developed to test the new theories being developed by physicists. New engineering concepts had to be developed and demonstrated in order to build facilities and equipment thatmore » had never before been attempted. Chem Tech`s role was chemical separations, especially uranium and plutonium, and nuclear fuel reprocessing. With diversification of national and ORNL missions, Chem Tech undertook R&D studies in many areas including biotechnology; clinical and environmental chemistry; nuclear reactors; safety regulations; effective and safe waste management and disposal; computer modeling and informational databases; isotope production; and environmental control. The changing mission of Chem Tech are encapsulated in the evolving activities.« less

Revealing the Role of Microbes in Controlling Contaminants

ScienceCinema

Williams, Kenneth Hurst

2018-05-11

In Rifle, Colorado, Berkeley Lab earth scientist, Kenneth Hurst Williams, highlights the role subsurface microbial communities can play in controlling the flow of contaminants in groundwater. The DOE Joint Genome Institute is a key collaborator in the research. Williams is Component Lead of Watershed Structure and Controls within Berkeley Lab's Genomes-to-Watershed Scientific Focus Area.

Frontiers in Laser Cooling, Single-Molecule Biophysics, and Energy Science: Remarks from Steve Chu at the Scientific Symposium Held in his Honor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chu, Steve

2008-08-30

Steve Chu, director of Lawrence Berkeley National Laboratory and recipient of the 1997 Nobel Prize, presents a talk at Frontiers in Laser Cooling, Single-Molecule Biophysics and Energy Science, a scientific symposium in his honor. The symposium was held August 30, 2008 in Berkeley.

Frontiers in Laser Cooling, Single-Molecule Biophysics, and Enrgy Science: A Talk from Leo Holberg and Allen Mills

DOE Office of Scientific and Technical Information (OSTI.GOV)

Holberg, Leo; Mills, Allen

2008-08-30

Leo Holberg and Allen Mills present a talk at Frontiers in Laser Cooling, Single-Molecule Biophysics and Energy Science, a scientific symposium honoring Steve Chu, director of Lawrence Berkeley National Laboratory and recipient of the 1997 Nobel Prize in Physics. The symposium was held August 30, 2008 in Berkeley.

Carbon Smackdown: Cookstoves for the developing world

ScienceCinema

Ashok Gadgil, Kayje Booker, and Adam Rausch

2017-12-09

In this June 30, 2010 Berkeley Lab summer lecture, learn how efficient cookstoves for the developing world — from Darfur to Ethiopia and beyond — are reducing carbon dioxide emissions, saving forests, and improving health. Berkeley Lab's Ashok Gadgil, Kayje Booker, and Adam Rausch discuss why they got started in this great challenge and what's next.

Integration Defended: Berkeley Unified's Strategy to Maintain School Diversity

ERIC Educational Resources Information Center

Chavez, Lisa; Frankenberg, Erica

2009-01-01

In June 2007, the Supreme Court limited the tools that school districts could use to voluntarily integrate schools. In the aftermath of the decision, educators around the country have sought models of successful plans that would also be legal. One such model may be Berkeley Unified School District's (BUSD) plan. Earlier this year, the California…

Revealing the Role of Microbes in Controlling Contaminants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Williams, Kenneth Hurst

2015-04-02

In Rifle, Colorado, Berkeley Lab earth scientist, Kenneth Hurst Williams, highlights the role subsurface microbial communities can play in controlling the flow of contaminants in groundwater. The DOE Joint Genome Institute is a key collaborator in the research. Williams is Component Lead of Watershed Structure and Controls within Berkeley Lab's Genomes-to-Watershed Scientific Focus Area.

Frontiers in Laser Cooling, Single-Molecule Biophysics, and Enrgy Science: A Talk from Leo Holberg and Allen Mills

ScienceCinema

Holberg, Leo; Mills, Allen

2018-05-07

Leo Holberg and Allen Mills present a talk at Frontiers in Laser Cooling, Single-Molecule Biophysics and Energy Science, a scientific symposium honoring Steve Chu, director of Lawrence Berkeley National Laboratory and recipient of the 1997 Nobel Prize in Physics. The symposium was held August 30, 2008 in Berkeley.

A Proposal to Eliminate the SAT in Berkeley Admissions. Research & Occasional Paper Series: CSHE.4.16

ERIC Educational Resources Information Center

Geiser, Saul

2016-01-01

The SAT is used for two purposes at the University of California. First is "eligibility": Determining whether applicants meet the minimum requirements for admission to the UC system. Second is "admissions selection": At high-demand campuses such as Berkeley, with many more eligible applicants than places available, test scores…

For Berkeley's Sports Endowment, a Goal of $1-Billion

ERIC Educational Resources Information Center

Keller, Josh

2009-01-01

Most athletics programs, if forced to raise $300-million to renovate a football stadium, would not set an ambitious endowment goal at the same time. The University of California at Berkeley is trying to do both. The university's California Memorial Stadium sits directly over an earthquake fault: it needs a major seismic retrofit that will take…

The principle of phase stability and the accelerator program at Berkeley, 1945--1954

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lofgren, E.J.

1994-07-01

The discovery of the Principle of Phase Stability by Vladimir Veksler and Edwin McMillian and the end of the war released a surge of accelerator activity at the Lawrence Berkeley Laboratory (then The University of California Radiation Laboratory). Six accelerators incorporating the Principle of Phase Stability were built in the period 1945--1954.

VIEW OF PIEDMONT AVENUE TRAFFIC CIRCLE AT INTERSECTION OF CHANNING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW OF PIEDMONT AVENUE TRAFFIC CIRCLE AT INTERSECTION OF CHANNING WAY VIEW OF 2401 PIEDMONT, ALPHA EPSILON PHI HOUSE BY RATCLIFF & RATCLIFF, 1958. SEEN FROM NW CORNER LOOKING SE. Photograph by Brian Grogan, July 8, 2007 - Piedmont Way & the Berkeley Property Tract, East of College Avenue between Dwight Way & U.C. Memorial Stadium, Berkeley, Alameda County, CA

Social Integration and School Violence in a Multiracial Northern High School.

ERIC Educational Resources Information Center

Marascuilo, Leonard A.; Dagenais, Fred

This study, conducted in Berkeley, California, was designed to test the hypothesis that socially integrated high school students would not be involved in interracial violence and conflict, while students who were socially isolated would. Data were collected by means of a questionnaire, sent to the 1970 graduating class of Berkeley High School,…

Berkeley Foundation for Opportunities in Information Technology: A Decade of Broadening Participation

ERIC Educational Resources Information Center

Crutchfield, Orpheus S. L.; Harrison, Christopher D.; Haas, Guy; Garcia, Daniel D.; Humphreys, Sheila M.; Lewis, Colleen M.; Khooshabeh, Peter

2011-01-01

The Berkeley Foundation for Opportunities in Information Technology is a decade-old endeavor to expose pre-college young women and underrepresented racial and ethnic minorities to the fields of computer science and engineering, and prepare them for rigorous, university-level study. We have served more than 150 students, and graduated more than 65…

VIEW OF PIEDMONT AVENUE TRAFFIC CIRCLE AT INTERSECTION OF CHANNING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW OF PIEDMONT AVENUE TRAFFIC CIRCLE AT INTERSECTION OF CHANNING WAY. SEEN FROM SW CORNER TOWARDS 2395 PIEDMONT, SIGMA PI HOUSE BY FREDERICK H. REIMERS, 1928. LOOKING NORTH. Photograph by Brian Grogan, July 8, 2007 - Piedmont Way & the Berkeley Property Tract, East of College Avenue between Dwight Way & U.C. Memorial Stadium, Berkeley, Alameda County, CA

Martinsburg-Berkeley County Public Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

ERIC Educational Resources Information Center

Hess, Therese M.

The Martinsburg-Berkeley County Public Library (West Virginia) conducted a project that involved recruitment, retention, coalition building, public awareness, training, basic literacy, collection development, tutoring, computer assisted, other technology, and English as a Second Language (ESL) programs. The project served a three-county community…

Berkeley Pact with a Swiss Company Takes Technology Transfer to a New Level.

ERIC Educational Resources Information Center

Blumenstyk, Goldie

1998-01-01

In search of increased support for graduate students in plant science and upgraded laboratories, the College of Plant and Microbial Biology, University of California Berkeley, offered the college's expertise in exchange for major financial backing from the single company making the best offer. The resulting five-year, $25-million alliance with one…

Berkeley Lab Scientist Co-Leads Breast Cancer Dream Team

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gray, Joe

2009-05-19

An $16.5 million, three-year grant to develop new and more effective therapies to fight breast cancer was awarded today to a multi-institutional Dream Team of scientists and clinicians that is co-led by Joe Gray, a renowned cancer researcher with the U.S. Department of Energys Lawrence Berkeley National Laboratory. http://newscenter.lbl.gov/

76 FR 28066 - Notice of Intent To Repatriate a Cultural Item: Phoebe A. Hearst Museum of Anthropology...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-13

... California, Berkeley, Berkeley, CA, that meets the definition of sacred object under 25 U.S.C. 3001. This... sacred object, and the museum agrees with all the evidence presented and will repatriate the object... object needed by traditional Native American religious leaders for the practice of traditional Native...

The University of California at Berkeley: An Emerging Global Research University

ERIC Educational Resources Information Center

Ma, Wanhua

2008-01-01

Federal government science policy and R&D investment are two major factors for the success of research universities in the United States. This case analysis examines how the University of California at Berkeley shifted from a regional to a globally oriented research university by the influence of federal government science policy and R&D…

Berkeley Lab Scientist Co-Leads Breast Cancer Dream Team

ScienceCinema

Gray, Joe

2017-12-27

An $16.5 million, three-year grant to develop new and more effective therapies to fight breast cancer was awarded today to a multi-institutional Dream Team of scientists and clinicians that is co-led by Joe Gray, a renowned cancer researcher with the U.S. Department of Energys Lawrence Berkeley National Laboratory. http://newscenter.lbl.gov/

Doing Much More with Less: Implementing Operational Excellence at UC Berkeley. Research & Occasional Paper Series: CSHE.10.13

ERIC Educational Resources Information Center

Szeri, Andrew J.; Lyons, Richard; Huston, Peggy; Wilton, John

2013-01-01

Universities are undergoing historic change, from the sharp downward shift in government funding to widespread demands to document performance. At the University of California Berkeley, this led to an operational change effort unlike any the university had ever attempted, dubbed Operational Excellence. The authors describe their experiences…

The University of California, Berkeley, and the Government. An Institutional Self Study.

ERIC Educational Resources Information Center

Bowker, Albert H.; Morgan, Patrick M.

The relationship between the University of California at Berkeley and the government is examined. Interviews with over 150 people on campus, ranging from leading administrators to a sample of faculty researchers to section heads in departments such as Personnel and Purchasing, as well as written reports by numerous offices supplied the data of…

ALTERNATE VIEW OF SIDEWALK AND WALL OF INDIGENOUS VOLCANIC RYOLITE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

ALTERNATE VIEW OF SIDEWALK AND WALL OF INDIGENOUS VOLCANIC RYOLITE EASTSIDE OF PIEDMONT AVENUE ADJACENT TO OAK GROVE AND CALIFORNIA MEMORIAL STADIUM. LOOKING NORTH. Photograph by Fredrica Drotos and Michael Kelly, June 29, 2006 - Piedmont Way & the Berkeley Property Tract, East of College Avenue between Dwight Way & U.C. Memorial Stadium, Berkeley, Alameda County, CA

Berkeley Lab's Cool Your School Program

ScienceCinema

Brady, Susan; Gilbert, Haley; McCarthy, Robert

2018-02-02

Cool Your School is a series of 6th-grade, classroom-based, science activities rooted in Berkeley Lab's cool-surface and cool materials research and aligned with California science content standards. The activities are designed to build knowledge, stimulate curiosity, and carry the conversation about human-induced climate change, and what can be done about it, into the community.

Requirements Analysis and Course Improvements for EO3502 Telecommunications Systems Engineering

DTIC Science & Technology

2005-03-01

California, Berkeley, School of Information Management and Systems The University of California, Berkeley (Cal) is a public, coeducational university...NAVAL POSTGRADUATE SCHOOL MONTEREY, CALIFORNIA THESIS REQUIREMENTS ANALYSIS AND COURSE IMPROVEMENTS FOR E03502 TELECOMMUNICATIONS SYSTEMS ENGINEERING...Postgraduate School ORGANIZATION REPORT Monterey, CA 93943-5000 NUMBER 9. SPONSORING /MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSORING

An Evaluation of the New Curriculum at the University of California, Berkeley, School of Optometry.

ERIC Educational Resources Information Center

Harris, Michael G.; Kashani, Sandy; Saroj, Namrata

2001-01-01

Evaluated the new curriculum at the University of California, Berkeley, School of Optometry by comparing the content of the new curriculum to the old curriculum and by surveying faculty and students regarding their opinion of the new curriculum. Findings indicated that the curriculum is successful in implementing desired changes, including reduced…

Reducing Our Carbon Footprint: A Low-Energy House in Berkeley, Kabul, and Washington DC (LBNL Science at the Theater)

ScienceCinema

Diamond, Rick [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

2018-05-14

How well can we assess and improve building energy performance in California homes? How much energy-and carbon-do homes use in other parts of the world? Rick Diamond, deputy group leader of the Berkeley Lab Energy Performance of Buildings Group, discusses change, global solutions, and the stories of three houses in Berkeley, Kabul (Afghanistan), and Washington, D.C. Diamond, who is also a senior advisor at the California Institute for Energy and Environment, investigates user interactions with the built environment for improved building energy performance. The group has studied a wide range of issues related to energy use in housing, including duct system efficiency, user behavior, and infiltration and ventilation measurements.

A community of educators: professional development for graduate students within the Berkeley Compass Project

NASA Astrophysics Data System (ADS)

Schwab, Josiah; Roth, Nathaniel; Berkeley Compass Project

2015-01-01

The Berkeley Compass Project is a self-formed group of graduate and undergraduate students in the physical sciences at UC Berkeley. Our goals are to improve undergraduate physics education, provide opportunities for professional development, and increase retention of students from populations typically underrepresented in the physical sciences. Graduate students, together with upper-level undergraduates, design and run all Compass programs. We strive to create a community of educators that incorporates best practices from the science education literature. Along the way, we develop experience in curriculum development, fundraising, grant writing, interfacing with university administration, and other aspects of running an effective organization. Our experience in Compass leaves us better poised to be successful researchers, teachers, and mentors.

Control system for the 2nd generation Berkeley AutoMounters (BAM2) at GM/CA CAT macromolecular crystallography beamlines

PubMed Central

Makarov, O.; Hilgart, M.; Ogata, C.; Pothineni, S.; Cork, C.

2011-01-01

GM/CA CAT at Sector 23 of the Advanced Photon Source (APS) is an NIH funded facility for crystallographic structure determination of biological macromolecules by X-ray diffraction. A second generation Berkeley automounter is being integrated into the beamline control system at the 23-BM experimental station. This new device replaces the previous all-pneumatic gripper motions with a combination of pneumatics and XYZ motorized linear stages. The latter adds a higher degree of flexibility to the robot including auto-alignment capability, accommodation of a larger capacity sample Dewar of arbitrary shape, and support for advanced operations such as crystal washing, while preserving the overall simplicity and efficiency of the Berkeley automounter design. PMID:21822343

NASA Tech Briefs, April 1999. Volume 23, No. 4

NASA Technical Reports Server (NTRS)

1999-01-01

Topics include: special coverage sections on automotive technology, and CAM and sections on electronic components and systems, software, materials, machinery/automation, physical sciences, and a special section of Electronic Tech Briefs and Motion Control Tech Briefs.

NASA Tech Briefs, December 2000. Volume 24, No. 12

NASA Technical Reports Server (NTRS)

2000-01-01

Topics include: special coverage sections on Imaging/Video/Display Technology, and sections on electronic components and systems, test and measurement, software, information sciences, and special sections of Electronics Tech Briefs and Motion Control Tech Briefs.

NASA Tech Briefs, October 2001. Volume 25, No. 10

NASA Technical Reports Server (NTRS)

2001-01-01

Topics include: special coverage section on composites and plastics, electronic components and systems, software, mechanics, physical sciences, information sciences, book and reports, and a special sections of Photonics Tech Briefs and Motion Control Tech Briefs.

NASA Tech Briefs, February 2002. Volume 26, No. 2

NASA Technical Reports Server (NTRS)

2002-01-01

Topics include:a technology focus on computers, electronic components and systems, software, materials, mechanics,physical sciences machinery, manufacturing/fabrication, mathematics, book and reports, motion control tech briefs and a special section on Photonics Tech Briefs.

How To Implement a Tech Prep Program Based on the Rhode Island Model. Tech Prep Associate Degree Program. Technical Programs. Business/Office Administration Programs. Allied Health/Dental Health Programs.

ERIC Educational Resources Information Center

Community Coll. of Rhode Island, Warwick.

This implementation guide contains information based on experiences that occurred during the development and implementation of the Rhode Island Tech Prep Model. It is intended to assist educators in addressing challenges and obstacles faced by the program early in the planning process. It begins with a rationale for tech prep. Rhode Island…

Digital Logistics

DTIC Science & Technology

2000-03-17

There will also be instances when we will need to take advantage of technological leaps made by our allies. The ’clash’ of high tech with low tech ...must be considered, especially as we move toward very high tech solutions to our military needs. The end goal is to create Army Forces that are...effect that science and technology today distort the military by substituting high - tech weapons that do not always work for more basic military

a Study on the Document Information Service of the National Agricultural Library for Agricultural Sci-Tech Innovation in China

NASA Astrophysics Data System (ADS)

Xu, Qian; Meng, Xianxue

This paper presents the significant function of the Chinese National Agricultural Library (CNAL) in the agricultural sci-tech innovation system in China, analyses the development of collection and service in the CNAL, explores the challenge towards sustain and develop information services for the agricultural sci-tech research and innovation, at last proposes the strategy for sci-tech document information service development.

Impact of newer self-monitoring technology and brief phone-based intervention on weight loss: a randomized pilot study

PubMed Central

Ross, Kathryn M.; Wing, Rena R.

2016-01-01

Objective Despite the proliferation of newer self-monitoring technology (e.g., activity monitors and smartphone apps), their impact on weight loss outside of structured in-person behavioral intervention is unknown. Methods A randomized, controlled pilot study was conducted to examine efficacy of self-monitoring technology, with and without phone-based intervention, on 6-month weight loss in adults with overweight and obesity. Eighty participants were randomized to receive standard self-monitoring tools (ST, n=26), technology-based self-monitoring tools (TECH, n=27), or technology-based tools combined with phone-based intervention (TECH+PHONE, n=27). All participants attended one introductory weight loss session and completed assessments at baseline, 3 months, and 6 months. Results Weight loss from baseline to 6 months differed significantly between groups p=.042; there was a trend for TECH+PHONE (−6.4±1.2kg) to lose more weight than ST (−1.3±1.2kg); weight loss in TECH (−4.1±1.4kg) was between ST and TECH+PHONE. Fewer ST (15%) achieved ≥5% weight losses compared to TECH and TECH+PHONE (44%), p=.039. Adherence to self-monitoring caloric intake was higher in TECH+PHONE than TECH or ST, ps<.05. Conclusion These results suggest use of newer self-monitoring technology plus brief phone-based intervention improves adherence and weight loss compared to traditional self-monitoring tools. Further research should determine cost-effectiveness of adding phone-based intervention when providing self-monitoring technology. PMID:27367614

NASA Tech Briefs, September 1998. Volume 22, No. 9

NASA Technical Reports Server (NTRS)

1998-01-01

Topics include: special coverage on data acquisition, also, electronic components and circuits, electronic systems, software, materials, mechanics, machinery/automation, physical sciences, information sciences, This issue contains a special sections of Electronics Tech Briefs and Motion Control Tech Briefs.

C. Judson King of UC Berkeley

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prausnitz, John

2005-06-01

In the middle of the UC Berkeley campus, next to the Main Library, South Hall is the last surviving building from the original campus, founded about 135 years ago. A tiny tree-shaded appendix to this venerated classical building houses Berkeley's Center for Studies in Higher Education, directed by C. Judson King, former Provost and Senior Vice President--Academic Affairs of the ten-campus University of California and long-time Professor of Chemical Engineering at Berkeley. Jud came to Berkeley in 1963 as assistant professor of chemical engineering, following receipt of a doctor's degree from MIT and a subsequent short appointment as director ofmore » the MIT chemical engineering practice school station at what was then Esso (now Exxon) in New Jersey. His undergraduate degree is from Yale. Starting with his MIT doctoral dissertation on gas absorption, Jud has devoted much of his professional career to separation processes. His teaching and research activities have been primarily concerned with separation of mixtures with emphasis on liquid-liquid extraction and drying. As a consultant to Procter and Gamble, he contributed to the technology of making instant coffee. His life-long activities in hiking and camping stimulated Jud's interest in the manufacture of freeze-dried foods (e.g. turkey meat) to minimize the weight of his hiking back-pack. Jud is internationally known not only for his many research publications but even more, for his acclaimed textbook ''Separation Processses'' (McGraw-Hill, second edition 1980) that is used in standard chemical engineering courses in the US and abroad.« less

78 FR 14359 - Verizon Business Networks Services, Inc., Specialist-Tech Customer Service, Philadelphia, PA...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-05

... Business Networks Services, Inc., Specialist-Tech Customer Service, Philadelphia, PA; Verizon Business Networks Services, Inc., Specialist-Tech Customer Service, Tampa, Florida; Amended Certification Regarding... Business Networks Services, Inc., Order Management Division, Philadelphia, Pennsylvania and Verizon...

A Report on the Status of Women Employed in the Library of the University of California, Berkeley, with Recommendations for Affirmative Action.

ERIC Educational Resources Information Center

Lipow, Anne; And Others

This report deals with discrimination against women employed as librarians and library assistants at the University of California, Berkeley Library. The report demonstrates that (1) library professional, technical and clerical personnel--both women and men--work in "women's occupations," and, therefore, are underpaid when compared to…

Reduced Chemical Kinetic Mechanisms for Hydrocarbon Fuels

DTIC Science & Technology

2006-01-01

Technologies Reaction Engineering International 77 West 200 South, Suite # 210 Salt Lake City, UT 84101 3Professor Department of Mechanical ... Engineering University of California, Berkeley Berkeley, CA 94720 4Program Leader for Computational Chemistry Lawrence Livermore National Laboratory...species by the error introduced by assuming they are in quasi-steady state. The reduced mechanisms have been compared to detailed chemistry calculations

THE "FREE SPEECH" CRISES AT BERKELEY, 1964-1965--SOME ISSUES FOR SOCIAL AND LEGAL RESEARCH.

ERIC Educational Resources Information Center

LUNSFORD, TERRY F.

AN EXAMINATION WAS MADE OF THE ISSUES AND EVENTS OF THE "FREE SPEECH" CRISES ON THE BERKELEY CAMPUS OF THE UNIVERSITY OF CALIFORNIA IN AN ATTEMPT TO PROVIDE THE BASIS FOR MORE SYSTEMATIC AND DISPASSIONATE STUDY OF CERTAIN ISSUES BEHIND THE STUDENT PROTESTS, AND TO STIMULATE SOCIAL AND LEGAL RESEARCH ON THESE ISSUES. FOLLOWING AN…

The East Bay Vegetation Management Consortium:\\ta subregional approach to resource management and planning

Treesearch

Tony Acosta

1995-01-01

Formed in response to the October 20, 1991, Oakland/Berkeley hills firestorm, the East Bay Vegetation Management Consortium (EBVMC) is a voluntary association of public agencies concerned with vegetation management and planning related to fire hazard reduction in the Oakland/ Berkeley hills. To date, a total of nine agencies are participating in the EBVMC, including...

Follow the Money: Engineering at Stanford and UC Berkeley during the Rise of Silicon Valley

ERIC Educational Resources Information Center

Adams, Stephen B.

2009-01-01

A comparison of the engineering schools at UC Berkeley and Stanford during the 1940s and 1950s shows that having an excellent academic program is necessary but not sufficient to make a university entrepreneurial (an engine of economic development). Key factors that made Stanford more entrepreneurial than Cal during this period were superior…

DETAIL VIEW OF SIDEWALKS AND TREES ON CHANNING WAY AT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF SIDEWALKS AND TREES ON CHANNING WAY AT INTERSECTION OF PIEDMONT AVENUE WITH 2395 PIEDMONT, SIGMA PI HOUSE BY FREDERICK H. REIMERS, 1928, LOOKING NW. Photograph by Fredrica Drotos and Michael Kelly, July 9, 2006 - Piedmont Way & the Berkeley Property Tract, East of College Avenue between Dwight Way & U.C. Memorial Stadium, Berkeley, Alameda County, CA

DETAIL VIEW OF PIEDMONT AVENUE TRAFFIC CIRCLE AT INTERSECTION OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF PIEDMONT AVENUE TRAFFIC CIRCLE AT INTERSECTION OF CHANNING WAY. SEEN FROM EAST SIDE OF CIRCLE LOOKING NORTH AT 2395 PIEDMONT, SIGMA PI HOUSE BY FREDERICK H. REIMERS, 1928. Photograph by Brian Grogan, July 8, 2007 - Piedmont Way & the Berkeley Property Tract, East of College Avenue between Dwight Way & U.C. Memorial Stadium, Berkeley, Alameda County, CA

A Tale of Three Campuses: Planning and Design in Response to the Cultural Heritages at Mills College, the University of California, Berkeley, and Stanford University

ERIC Educational Resources Information Center

Fiene, Karen; Sabbatini, Robert

2011-01-01

How do forward-looking institutions with rich landscape and architectural heritages integrate contemporary programming and design? This article explores the evolution of the Mills College campus and compares it with two larger western universities: the University of California, Berkeley (UCB) and Leland Stanford, Jr., University (Stanford…

Berkeley extreme-ultraviolet airglow rocket spectrometer - BEARS

NASA Technical Reports Server (NTRS)

Cotton, D. M.; Chakrabarti, S.

1992-01-01

The Berkeley EUV airglow rocket spectrometer (BEARS) instrument is described. The instrument was designed in particular to measure the dominant lines of atomic oxygen in the FUV and EUV dayglow at 1356, 1304, 1027, and 989 A, which is the ultimate source of airglow emissions. The optical and mechanical design of the instrument, the detector, electronics, calibration, flight operations, and results are examined.

Survey of Spring 1982 Graduating Seniors in the College of Engineering, University of California, Berkeley.

ERIC Educational Resources Information Center

Thomson, Gregg E.

Opinions and experiences of college seniors majoring in engineering at the University of California, Berkeley, were studied in spring 1982. Specific attention was focused on the unequal distribution of interest in the various engineering programs. Data were analyzed by program, year of entry, and commitment to engineering in general and/or one's…

Behind the Scenes at Berkeley Lab - The Mechanical Fabrication Facility

ScienceCinema

Wells, Russell; Chavez, Pete; Davis, Curtis; Bentley, Brian

2018-04-16

Part of the Behind the Scenes series at Berkeley Lab, this video highlights the lab's mechanical fabrication facility and its exceptional ability to produce unique tools essential to the lab's scientific mission. Through a combination of skilled craftsmanship and precision equipment, machinists and engineers work with scientists to create exactly what's needed - whether it's measured in microns or meters.

Projects made with the Berkeley Lab Circuit Board

Science.gov Websites

dependence of cosmic rays. Greg Poe, a student at Travis High School in Richmond, Texas, received an the journal Physics Education. He used the Berkeley Lab circuit board together with spare parts from New York Schools Cosmic Particle Telescope workshop. Ken Cecire has created a web page which describes

Solar Fridges and Personal Power Grids: How Berkeley Lab is Fighting Global Poverty (LBNL Science at the Theater)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Buluswar, Shashi; Gadgil, Ashok

At this November 26, 2012 Science at the Theater, scientists discussed the recently launched LBNL Institute for Globally Transformative Technologies (LIGTT) at Berkeley Lab. LIGTT is an ambitious mandate to discover and develop breakthrough technologies for combating global poverty. It was created with the belief that solutions will require more advanced R&D and a deep understanding of market needs in the developing world. Berkeley Lab's Ashok Gadgil, Shashi Buluswar and seven other LIGTT scientists discussed what it takes to develop technologies that will impact millions of people. These include: 1) Fuel efficient stoves for clean cooking: Our scientists are improvingmore » the Berkeley Darfur Stove, a high efficiency stove used by over 20,000 households in Darfur; 2) The ultra-low energy refrigerator: A lightweight, low-energy refrigerator that can be mounted on a bike so crops can survive the trip from the farm to the market; 3) The solar OB suitcase: A low-cost package of the five most critical biomedical devices for maternal and neonatal clinics; 4) UV Waterworks: A device for quickly, safely and inexpensively disinfecting water of harmful microorganisms.« less

Trans-ethnic meta-analysis of genome-wide association studies for Hirschsprung disease.

PubMed

Tang, Clara Sze-Man; Gui, Hongsheng; Kapoor, Ashish; Kim, Jeong-Hyun; Luzón-Toro, Berta; Pelet, Anna; Burzynski, Grzegorz; Lantieri, Francesca; So, Man-Ting; Berrios, Courtney; Shin, Hyoung Doo; Fernández, Raquel M; Le, Thuy-Linh; Verheij, Joke B G M; Matera, Ivana; Cherny, Stacey S; Nandakumar, Priyanka; Cheong, Hyun Sub; Antiñolo, Guillermo; Amiel, Jeanne; Seo, Jeong-Meen; Kim, Dae-Yeon; Oh, Jung-Tak; Lyonnet, Stanislas; Borrego, Salud; Ceccherini, Isabella; Hofstra, Robert M W; Chakravarti, Aravinda; Kim, Hyun-Young; Sham, Pak Chung; Tam, Paul K H; Garcia-Barceló, Maria-Mercè

2016-12-01

Hirschsprung disease (HSCR) is the most common cause of neonatal intestinal obstruction. It is characterized by the absence of ganglia in the nerve plexuses of the lower gastrointestinal tract. So far, three common disease-susceptibility variants at the RET, SEMA3 and NRG1 loci have been detected through genome-wide association studies (GWAS) in Europeans and Asians to understand its genetic etiologies. Here we present a trans-ethnic meta-analysis of 507 HSCR cases and 1191 controls, combining all published GWAS results on HSCR to fine-map these loci and narrow down the putatively causal variants to 99% credible sets. We also demonstrate that the effects of RET and NRG1 are universal across European and Asian ancestries. In contrast, we detected a European-specific association of a low-frequency variant, rs80227144, in SEMA3 [odds ratio (OR) = 5.2, P = 4.7 × 10-10]. Conditional analyses on the lead SNPs revealed a secondary association signal, corresponding to an Asian-specific, low-frequency missense variant encoding RET p.Asp489Asn (rs9282834, conditional OR = 20.3, conditional P = 4.1 × 10-14). When in trans with the RET intron 1 enhancer risk allele, rs9282834 increases the risk of HSCR from 1.1 to 26.7. Overall, our study provides further insights into the genetic architecture of HSCR and has profound implications for future study designs. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Neuropilin 1 Receptor Is Up-Regulated in Dysplastic Epithelium and Oral Squamous Cell Carcinoma.

PubMed

Shahrabi-Farahani, Shokoufeh; Gallottini, Marina; Martins, Fabiana; Li, Erik; Mudge, Dayna R; Nakayama, Hironao; Hida, Kyoko; Panigrahy, Dipak; D'Amore, Patricia A; Bielenberg, Diane R

2016-04-01

Neuropilins are receptors for disparate ligands, including proangiogenic factors such as vascular endothelial growth factor and inhibitory class 3 semaphorin (SEMA3) family members. Differentiated cells in skin epithelium and cutaneous squamous cell carcinoma highly express the neuropilin-1 (NRP1) receptor. We examined the expression of NRP1 in human and mouse oral mucosa. NRP1 was significantly up-regulated in oral epithelial dysplasia and oral squamous cell carcinoma (OSCC). NRP1 receptor localized to the outer suprabasal epithelial layers in normal tongue, an expression pattern similar to the normal skin epidermis. However, dysplastic tongue epithelium and OSCC up-regulated NRP1 in basal and proliferating epithelial layers, a profile unseen in cutaneous squamous cell carcinoma. NRP1 up-regulation is observed in a mouse carcinogen-induced OSCC model and in human tongue OSCC biopsies. Human OSCC cell lines express NRP1 protein in vitro and in mouse tongue xenografts. Sites of capillary infiltration into orthotopic OSCC tumors correlate with high NRP1 expression. HSC3 xenografts, which express the highest NRP1 levels of the cell lines examined, showed massive intratumoral lymphangiogenesis. SEMA3A inhibited OSCC cell migration, suggesting that the NRP1 receptor was bioactive in OSCC. In conclusion, NRP1 is regulated in the oral epithelium and is selectively up-regulated during epithelial dysplasia. NRP1 may function as a reservoir to sequester proangiogenic ligands within the neoplastic compartment, thereby recruiting neovessels toward tumor cells. Copyright © 2016 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

76 FR 68243 - Youth Leadership Program: TechGirls

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-03

... DEPARTMENT OF STATE [Public Notice: 7671] Youth Leadership Program: TechGirls Overview Information Bureau of Educational and Cultural Affairs (ECA) Request for Grant Proposals: Youth Leadership Program... Cultural Affairs (ECA) announces an open competition for the new Youth Leadership Program ``TechGirls...

Materials Evaluation in the Tri-Service Thermal Radiation Test Facility.

DTIC Science & Technology

1984-06-20

CONTRACTORS (Continued) Science Applications Intl Corp Science Applications. Inc ATTN: S. Binninger ATTN: Tech Library Science Applications Intl Corp...Tech, Inc ATTN: Library Science Applications, Inc ATTN: Tech Library TRW Electronics & Defense Sector ATTN: h. Plows ATTN: B. Sussholtz ATTN: J

"What I Did over My Summer Vacation..."

ERIC Educational Resources Information Center

Techniques: Making Education and Career Connections, 1997

1997-01-01

A tech prep summer camp was designed to give ninth graders a taste of tech prep before they were asked to choose a high school path. Parents were invited to dinner to learn about tech prep programs and their potential for successful careers. (JOW)

LUVOIR Tech Notes

NASA Technical Reports Server (NTRS)

Bolcar, Matthew R.; Shaklan, Stuart; Roberge, Aki; Rioux, Norman; Feinberg, Lee; Werner, Michael; Rauscher, Bernard; Mandell, Avi; France, Kevin; Schiminovich, David

2016-01-01

We present nine "tech notes" prepared by the Large UV/Optical/Infrared (LUVOIR) Science and Technology Definition Team (STDT), Study Office, and Technology Working Group. These tech notes are intended to highlight technical challenges that represent boundaries in the trade space for developing the LUVOIR architecture that may impact the science objectives being developed by the STDT. These tech notes are intended to be high-level discussions of the technical challenges and will serve as starting points for more in-depth analysis as the LUVOIR study progresses.

Barriers to communication and cooperation in addressing community impacts of radioactive releases from research facilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Harrach, Robert J.; Peterson, S. Ring

1999-05-05

Two instances of research facilities responding to public scrutiny will be discussed. The first concerns emissions from a "tritium labeling facility" operated at Lawrence Berkeley National Laboratory (LBNL); the second deals with releases of plutonium from Lawrence Livermore National Laboratory (LLNL). There are many parallels between these two cases, both of which are still ongoing. In both, the national laboratory is the acknowledged source of low-level (by regulatory standards) radioactive contamination in the community. A major purpose of both investigations is to determine the degree of the contamination and the threat it poses to public health and the environment. Themore » examining panel or committee is similarly constituted in the two cases, including representatives from all four categories of stakeholders: decision makers; scientists and other professionals doing the analysis/assessment; environmental activist or public interest groups; and "ordinary" citizens (nearly everyone else not in one or more of the first three camps). Both involved community participation from the beginning. The levels of outrage over the events triggering the assessment are comparable; though "discovered" or "appreciated" only a few years ago, the release of radiation in both cases occurred or began occurring more than a decade ago. The meetings have been conducted in a similar manner, with comparable frequency, often utilizing the services of professional facilitators. In both cases, the sharply contrasting perceptions of risk commonly seen between scientists and activists were present from the beginning, though the contrast was sharper and more problematical in the Berkeley case. Yet, the Livermore case seems to be progressing towards a satisfactory resolution, while the Berkeley case remains mired in ill-will, with few tangible results after two years of effort. We perceive a wide gap in negotiation skills (at the very least), and a considerable difference in willingness to compromise, between the environmental activist groups participating in the two cases. A degree of contentiousness existed from the start among the participants in the Berkeley case particularly between the environmental activists and the scientists/regulators that was not approached in the Livermore case, and which was and still is severe enough to stifle meaningful progress. The Berkeley activists are considerably more aggressive, we believe, in arguing their points of view, making demands about what should be done, and verbally assailing the scientists and government regulators. We offer the following comments on the barriers to communication and cooperation that distinguish the Berkeley and Livermore cases. In no particular order, they are (a) the presence of a higher degree of polarization between the Berkeley activists and the "establishment," as represented by government scientists and regulators, (b) the absence, in the Berkeley case, of an activist leader with skills and effectiveness comparable to a well-known leader in Livermore, (c) frequent displays by several of the Berkeley activists of incivility, distrust, and disrespect for the regulators and scientists, (d) extraordinary difficulties in reaching consensus in the Tritium Issues Work Group meetings, perhaps because goals diverged among the factions, (e) a considerable degree of resentment by the Berkeley activists over the imbalance in conditions of participation, pitting well-paid, tax-supported professionals against "citizen volunteers," (f) the brick wall that divides the perspectives of "no safe dose" and "levels below regulatory concern" when trying to reach conclusions about radiation dangers to the community, and (g) unwillingness to consider both sides of the risk-reward coin: benefits to the community and society at large of the tritium labeling activity, vs. the health risk from small quantities of tritium released to the environment.« less

Longitudinal analysis of high-technology medical services and hospital financial performance.

PubMed

Zengul, Ferhat D; Weech-Maldonado, Robert; Ozaydin, Bunyamin; Patrician, Patricia A; OʼConnor, Stephen J

U.S. hospitals have been investing in high-technology medical services as a strategy to improve financial performance. Despite the interest in high-tech medical services, there is not much information available about the impact of high-tech services on financial performance. The aim of this study was to examine the impact of high-tech medical services on financial performance of U.S. hospitals by using the resource-based view of the firm as a conceptual framework. Fixed-effects regressions with 2 years lagged independent variables using a longitudinal panel sample of 3,268 hospitals (2005-2010). It was hypothesized that hospitals with rare or large numbers (breadth) of high-tech medical services will experience better financial performance. Fixed effects regression results supported the link between a larger breadth of high-tech services and total margin, but only among not-for-profit hospitals. Both breadth and rareness of high-tech services were associated with high total margin among not-for-profit hospitals. Neither breadth nor rareness of high-tech services was associated with operating margin. Although breadth and rareness of high-tech services resulted in lower expenses per inpatient day among not-for-profit hospitals, these lower costs were offset by lower revenues per inpatient day. Enhancing the breadth of high-tech services may be a legitimate organizational strategy to improve financial performance, especially among not-for-profit hospitals. Hospitals may experience increased productivity and efficiency, and therefore lower inpatient operating costs, as a result of newer technologies. However, the negative impact on operating revenue should caution hospital administrators about revenue reducing features of these technologies, which may be related to the payer mix that these technologies may attract. Therefore, managers should consider both the cost and revenue implications of these technologies.

THE YOUNG OPEN CLUSTER BERKELEY 55

DOE Office of Scientific and Technical Information (OSTI.GOV)

Negueruela, Ignacio; Marco, Amparo, E-mail: ignacio.negueruela@ua.es, E-mail: amparo.marco@ua.es

We present UBV photometry of the highly reddened and poorly studied open cluster Berkeley 55, revealing an important population of B-type stars and several evolved stars of high luminosity. Intermediate-resolution far-red spectra of several candidate members confirm the presence of one F-type supergiant and six late supergiants or bright giants. The brightest blue stars are mid-B giants. Spectroscopic and photometric analyses indicate an age 50 {+-} 10 Myr. The cluster is located at a distance d Almost-Equal-To 4 kpc, consistent with other tracers of the Perseus Arm in this direction. Berkeley 55 is thus a moderately young open cluster withmore » a sizable population of candidate red (super)giant members, which can provide valuable information about the evolution of intermediate-mass stars.« less

Environmental assessment for the proposed construction and operation of a Genome Sequencing Facility in Building 64 at Lawrence Berkeley Laboratory, Berkeley, California

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1995-04-01

This document is an Environmental Assessment (EA) for a proposed project to modify 14,900 square feet of an existing building (Building 64) at Lawrence Berkeley Laboratory (LBL) to operate as a Genome Sequencing Facility. This EA addresses the potential environmental impacts from the proposed modifications to Building 64 and operation of the Genome Sequencing Facility. The proposed action is to modify Building 64 to provide space and equipment allowing LBL to demonstrate that the Directed DNA Sequencing Strategy can be scaled up from the current level of 750,000 base pairs per year to a facility that produces over 6,000,000 basemore » pairs per year, while still retaining its efficiency.« less

A Community of Scientists and Educators: The Compass Project at UC Berkeley

NASA Astrophysics Data System (ADS)

Roth, Nathaniel; Schwab, Josiah

2016-01-01

The Berkeley Compass Project is a self-formed group of graduate and undergraduate students in the physical sciences at the University of California, Berkeley. Its goals are to improve undergraduate physics education, provide opportunities for professional development, and increase retention of students from populations underrepresented in the physical sciences. For undergraduate students, the core Compass experience consists of a summer program and several seminar courses. These programs are designed to foster a diverse, collaborative student community in which students engage in authentic research practices and regular self-reflection. Graduate students, together with upper-level undergraduates, design and run all Compass programs. Compass strives to incorporate best practices from the science education literature. Experiences in Compass leave participants poised to be successful students researchers, teachers, and mentors.

Biodegradation of tech-hexachlorocyclohexane in a upflow anaerobic sludge blanket (UASB) reactor.

PubMed

Bhat, Praveena; Kumar, M Suresh; Mudliar, Sandeep N; Chakrabarti, T

2006-04-01

Biodegradability of technical grade hexachlorocyclohexane (tech-HCH) was studied in an upflow anaerobic sludge blanket reactor (UASB) under continuous mode of operation in concentration range of 100-200 mg/l and constant HRT of 48 h. At steady state operation more than 85% removal of tech-HCH (upto 175 mg/l concentration) and complete disappearance of beta-HCH was observed. Kinetic constants in terms of maximum specific tech-HCH utilization rate (k) and half saturation velocity constant (K(L)) were found to be 11.88 mg/g/day and 8.11 mg/g/day, respectively. The tech-HCH degrading seed preparation, UASB reactor startup and degradation in continuous mode of operation of the reactor is presented in this paper.

Laboratory Directed Research and Development Program FY 2008 Annual Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

editor, Todd C Hansen

2009-02-23

The Ernest Orlando Lawrence Berkeley National Laboratory (Berkeley Lab or LBNL) is a multi-program national research facility operated by the University of California for the Department of Energy (DOE). As an integral element of DOE's National Laboratory System, Berkeley Lab supports DOE's missions in fundamental science, energy resources, and environmental quality. Berkeley Lab programs advance four distinct goals for DOE and the nation: (1) To perform leading multidisciplinary research in the computing sciences, physical sciences, energy sciences, biosciences, and general sciences in a manner that ensures employee and public safety and protection of the environment. (2) To develop and operatemore » unique national experimental facilities for qualified investigators. (3) To educate and train future generations of scientists and engineers to promote national science and education goals. (4) To transfer knowledge and technological innovations and to foster productive relationships among Berkeley Lab's research programs, universities, and industry in order to promote national economic competitiveness. Berkeley Lab's research and the Laboratory Directed Research and Development (LDRD) program support DOE's Strategic Themes that are codified in DOE's 2006 Strategic Plan (DOE/CF-0010), with a primary focus on Scientific Discovery and Innovation. For that strategic theme, the Fiscal Year (FY) 2008 LDRD projects support each one of the three goals through multiple strategies described in the plan. In addition, LDRD efforts support the four goals of Energy Security, the two goals of Environmental Responsibility, and Nuclear Security (unclassified fundamental research that supports stockpile safety and nonproliferation programs). The LDRD program supports Office of Science strategic plans, including the 20-year Scientific Facilities Plan and the Office of Science Strategic Plan. The research also supports the strategic directions periodically under consideration and review by the Office of Science Program Offices, such as LDRD projects germane to new research facility concepts and new fundamental science directions. Berkeley Lab LDRD program also play an important role in leveraging DOE capabilities for national needs. The fundamental scientific research and development conducted in the program advances the skills and technologies of importance to our Work For Others (WFO) sponsors. Among many directions, these include a broad range of health-related science and technology of interest to the National Institutes of Health, breast cancer and accelerator research supported by the Department of Defense, detector technologies that should be useful to the Department of Homeland Security, and particle detection that will be valuable to the Environmental Protection Agency. The Berkeley Lab Laboratory Directed Research and Development Program FY2008 report is compiled from annual reports submitted by principal investigators following the close of the fiscal year. This report describes the supported projects and summarizes their accomplishments. It constitutes a part of the LDRD program planning and documentation process that includes an annual planning cycle, project selection, implementation, and review.« less

GT | Government & Community Relations | Georgia Institute of Technology |

Science.gov Websites

Atlanta, GA Skip to content Georgia Tech Georgia Institute of Technology Government & Georgia Tech's Impact State Relations Capitol Jackets Advocacy Network Legislative Priorities & ; Resources Georgia Legislative Internship Program Georgia Tech's Impact 2018 PHELAP Conference Community &

NASA Tech Briefs, November 1998. Volume 22, No. 11

NASA Technical Reports Server (NTRS)

1998-01-01

Topics include: special coverage sections on test and measurement and sections on electronic components and circuits, electronic systems, software, materials, mechanics, machinery/automation, physical sciences, information sciences, book and reports, and special sections of Electronics Tech Briefs amd Rapid Product Development Tech Briefs.

A Mobile Phone Based Method to Assess Energy and Food Intake in Young Children: A Validation Study against the Doubly Labelled Water Method and 24 h Dietary Recalls.

PubMed

Delisle Nyström, Christine; Forsum, Elisabet; Henriksson, Hanna; Trolle-Lagerros, Ylva; Larsson, Christel; Maddison, Ralph; Timpka, Toomas; Löf, Marie

2016-01-15

Mobile phones are becoming important instruments for assessing diet and energy intake. We developed the Tool for Energy Balance in Children (TECH), which uses a mobile phone to assess energy and food intake in pre-school children. The aims of this study were: (a) to compare energy intake (EI) using TECH with total energy expenditure (TEE) measured via doubly labelled water (DLW); and (b) to compare intakes of fruits, vegetables, fruit juice, sweetened beverages, candy, ice cream, and bakery products using TECH with intakes acquired by 24 h dietary recalls. Participants were 39 healthy, Swedish children (5.5 ± 0.5 years) within the ongoing Mobile-based Intervention Intended to Stop Obesity in Preschoolers (MINISTOP) obesity prevention trial. Energy and food intakes were assessed during four days using TECH and 24 h telephone dietary recalls. Mean EI (TECH) was not statistically different from TEE (DLW) (5820 ± 820 kJ/24 h and 6040 ± 680 kJ/24 h, respectively). No significant differences in the average food intakes using TECH and 24 h dietary recalls were found. All food intakes were correlated between TECH and the 24 h dietary recalls (ρ = 0.665-0.896, p < 0.001). In conclusion, TECH accurately estimated the average intakes of energy and selected foods and thus has the potential to be a useful tool for dietary studies in pre-school children, for example obesity prevention trials.

Tech Prep Compendium of Models. [Revised].

ERIC Educational Resources Information Center

Delaware Technical and Community Coll., Dover.

This publication discusses four models for technical preparation (tech prep): program organization; student progress; tech prep data collection and evaluation model; and school/community. The program organization model is divided into four sections. Section I, the business industry, and labor section, shows the flow from craft committee and…

NASA Tech Briefs, June 1998. Volume 22, No. 6

NASA Technical Reports Server (NTRS)

1998-01-01

Topics include: special coverage on computer hardware and peripherals, electronic components and circuits, electronic systems, software, materials, mechanics, machinery/automation, manufacturing, physical sciences, information sciences, book and reports, and a special section of Photonics Tech Briefs. and a second special section of Motion Control Tech Briefs

Health Technologies State Tech Prep Competency Profile.

ERIC Educational Resources Information Center

Ohio State Univ., Columbus. Center on Education and Training for Employment.

This "tech prep" state competency profile contains all the competencies required and recommended for entry-level employees in occupations in the health technologies cluster. Introductory materials include the following: descriptions of the different types of competencies (essential ones that must be included in all new tech prep programs…

NASA Tech Briefs, May 1999. Volume 23, No. 5

NASA Technical Reports Server (NTRS)

1999-01-01

Topics include: special coverage sections on sensors and composites and plastics, and sections on electronic components and systems, software, materials, mechanics, machinery and automation, bio-medical, physical sciences, book and reports, and a special section of Photonics Tech Briefs and Rapid Product Development Tech Briefs.

NASA Tech Briefs, October 1999. Volume 23, No. 10

NASA Technical Reports Server (NTRS)

1999-01-01

Topics include: special coverage section on data acquisition and sensors and sections on electronic components and systems, software, materials, mechanics, machinery/automation, manufacturing/fabrication, bio-medical, physical sciences, information sciences, book and reports, and special section of Electronics Tech Briefs and Motion Control Tech briefs

Planning a Successful Tech Show

ERIC Educational Resources Information Center

Nikirk, Martin

2011-01-01

Tech shows are a great way to introduce prospective students, parents, and local business and industry to a technology and engineering or career and technical education program. In addition to showcasing instructional programs, a tech show allows students to demonstrate their professionalism and skills, practice public presentations, and interact…

Fast Surface Reconstruction and Segmentation with Terrestrial LiDAR Range Data

DTIC Science & Technology

2009-05-18

UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) University of California at Berkeley,Department of Electrical Engineering and Computer...Sciences,Berkeley,CA,94720 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S...ACRONYM(S) 11. SPONSOR/MONITOR’S REPORT NUMBER(S) 12. DISTRIBUTION /AVAILABILITY STATEMENT Approved for public release; distribution unlimited 13

The Vicious Cycle of Gender and Status at the University of California at Berkeley, 1918-1954. ASHE 1988 Annual Meeting Paper.

ERIC Educational Resources Information Center

Nerad, Maresi

The way in which an all-women's department, the Department of Home Economics at the University of California (Berkeley), tried to raise its status and adhere to academic values of a research university after starting out as a low prestige undergraduate program is analyzed. Some of the related research questions are: whether academic departments…

DOE Office of Scientific and Technical Information (OSTI.GOV)

Haber, Carl

Summer Lecture Series 2006: Physicist Carl Haber and colleagues have found a way to digitize century-old recordings believed to be unplayable, and as a result, some of the music and spoken word recordings in the Library of Congress collection may spring back to life. Learn how basic scientific research done at Berkeley Lab may yield results of benefit in other areas of science and culture. Series: "Lawrence Berkeley National Laboratory Summer Lecture Series"

Shut out of the System: As Competition Increases for Slots at UC-Berkeley, Admission Offers to Minority Students Continue to Decline

ERIC Educational Resources Information Center

Burdman, Pamela

2004-01-01

As University of California officials announced admissions results for the fall, it appeared that increasing competition for seats at the university, rising tuition costs, and continued controversy over the role of race in admissions were conspiring to reduce the slots offered to African American students at UC-Berkeley. At the same time, the…

Pump for spawning channels includes a turbine and motor. Turbine ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Pump for spawning channels includes a turbine and motor. Turbine is Berkeley H-17500, model 8C2PH, Serial No. 2889, B.M. No. 4886 - Berkeley Pump Co. The Motor is G.E. Induction Motor, model 5K4256XA3YI, serial no. GAJ728337, Tri-Clad. View looking northeast. - Prairie Creek Fish Hatchery, Hwy. 101, Orick, Humboldt County, CA

Map showing bottom topography of the Pacific Continental Margin, Cape Mendocino to Point Conception

USGS Publications Warehouse

Chase, T.E.; Wilde, Pat; Normark, W.R.; Evenden, G.I.; Miller, C.P.; Seekins, B.A.; Young, J. D.; Grim, M.S.; Lief, C.J.

1992-01-01

Wilde, Pat, Chase, T.E., Holmes, M.L., Normark, W.R., Thomas, J.A., McCulloch, D.S., and Kulm, L.D., 1978, Oceanographic data off northern California-southern Oregon 40° to 43° North including the Gorda Deep Sea Fan: Berkeley, University of California, Lawrence Berkeley Laboratory Publication 251, scale 1:815,482 at 42° latitude.

Peacebook: A Resource Guide for Peace Studies at U.C. Berkeley, 1982-83 [and] 1983-84.

ERIC Educational Resources Information Center

Peace Studies Student Association.

A series of essays and lists of resource materials as well as courses relevant to peace studies at the University of California Berkeley are presented in the two guides. Topics of the 1982-83 guide include the undergraduate major in Peace and Conflict Studies (PACS), a proposed curriculum, core courses, concentration area courses (social systems,…

Strategic directions and mechanisms in technology transfer

NASA Technical Reports Server (NTRS)

Mackin, Robert

1992-01-01

An outline summarizing the Working Panel discussion related to strategic directions for technology transfer is presented. Specific topics addressed include measuring success, management of technology, innovation and experimentation in the tech transfer process, integration of tech transfer into R&D planning, institutionalization of tech transfer, and policy/legislative resources.

Adult Tech Prep.

ERIC Educational Resources Information Center

Schaad, Donna

For over 2 years, Blak Hawk College (Illinois) has provided high school equivalency (GED) candidates and recipients, older returning students, and underprepared high school graduates with a Tech Prep curriculum to give them the skills to make the transition from adult basic education to college or work. The Adult Tech Prep (ATP) core curriculum…

Promoting Tech Schools within Your Community

ERIC Educational Resources Information Center

Nikirk, Martin

2010-01-01

This article describes a number of promotional events and practices that the author's school engages in. The author hopes that his school's experiences will serve as an inspiration and useful model for other "Tech Directions" readers. There are many methods for successfully promoting one's tech program. Two key strategies are: (1) Connect in…

Contextual Learning and Tech Prep Curriculum Integration.

ERIC Educational Resources Information Center

Edling, Walter

Because tech prep has the twin goals of preparing students for entry into postsecondary/continuing education or the work force after high school, tech prep programs require significant modification of conventional curricula and teaching methodologies. Both research and experience have demonstrated that the ability to transfer learning from one…

Minimizing Security Vulnerabilities in High-Tech Classrooms

ERIC Educational Resources Information Center

Ozkan, Betul C.; Gunay, Vedat

2004-01-01

Emerging technologies are quickly becoming part of daily learning and teaching endeavors in academia. Due to the access to certain high-tech tools educators must learn how to integrate these tools in educational settings. However, many also encounter problems and weaknesses in the same high-tech environment that uses and delivers information…

NASA Tech Briefs, December 1998. Volume 22, No. 12

NASA Technical Reports Server (NTRS)

1998-01-01

Topics include: special coverage section on design and analysis software, and sections on electronic components and circuits, electronic systems, software, materials, mechanics, machinery/automation, manufacturing/fabrication, physical sciences, and special sections of Photonics Tech Briefs, Motion Control Tech briefs and a Hot Technology File 1999 Resource Guide.

NASA Tech Briefs, October 2000. Volume 24, No. 10

NASA Technical Reports Server (NTRS)

2000-01-01

Topics include: special coverage sections on CAD, CAE, and PDM, and, Composites and Plastics, and sections on electronic components and systems, software, test and measurement, mechanics, manufacturing/fabrication, physical sciences, information sciences, book and reports, and special sections of Electronics Tech Briefs and Motion Control Tech Briefs

Tech-Prep Competency Profiles within the Business/Computer Technologies.

ERIC Educational Resources Information Center

Ohio State Univ., Columbus. Center on Education and Training for Employment.

This document, which is designed for educators throughout Ohio who are involved in planning and/or delivering tech prep programs within the business/computer technologies cluster, discusses and presents tech prep competency profiles (TCPs) for 12 business/computer technology occupations. The first part of the document contains the following:…

75 FR 76036 - American Axle & Manufacturing Detroit Manufacturing Complex Holbrook Avenue and Saint Aubin...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-07

... From Paint Tech International Detroit, MI; Amended Certification Regarding Eligibility To Apply for... firm. New information revealed that workers leased from Paint Tech International were employed on-site... certification to include workers leased from Paint Tech International working on-site at the Detroit, Michigan...

Design Tech High School: d.tech

ERIC Educational Resources Information Center

EDUCAUSE, 2015

2015-01-01

A Bay Area charter high school, d.tech develops "innovation-ready" students by combining content knowledge with the design thinking process while fostering a sense of autonomy and purpose. The academic model is grounded in self-paced learning through a flex schedule, high standards, and design thinking through a four-year design…

School-to-Work Apprenticeship. Project Manual 1993-1994.

ERIC Educational Resources Information Center

Lee Coll., Baytown, TX.

With Perkins tech prep funds, Lee College (Baytown, Texas), working with the Gulf Coast Tech Prep Consortium and the Goose Creek Consolidated Independent School District, developed a school-to-work apprenticeship model for tech prep programs. An advisory committe provided guidance in identifying targeted apprenticeable jobs, program content, and…

Understanding transferable supply chain lessons and practices to a "high-tech" industry using guidelines from a primary sector industry: a case study in the food industry supply chain.

PubMed

Coronado Mondragon, Adrian E; Coronado Mondragon, Christian E; Coronado, Etienne S

2015-01-01

Flexibility and innovation at creating shapes, adapting processes, and modifying materials characterize composites materials, a "high-tech" industry. However, the absence of standard manufacturing processes and the selection of materials with defined properties hinder the configuration of the composites materials supply chain. An interesting alternative for a "high-tech" industry such as composite materials would be to review supply chain lessons and practices in "low-tech" industries such as food. The main motivation of this study is to identify lessons and practices that comprise innovations in the supply chain of a firm in a perceived "low-tech" industry that can be used to provide guidelines in the design of the supply chain of a "high-tech" industry, in this case composite materials. This work uses the case study/site visit with analogy methodology to collect data from a Spanish leading producer of fresh fruit juice which is sold in major European markets and makes use of a cold chain. The study highlights supply base management and visibility/traceability as two elements of the supply chain in a "low-tech" industry that can provide guidelines that can be used in the configuration of the supply chain of the composite materials industry.

Museum in a School (reaching the unreachable audience). Final report, February 2, 1993--December 14, 1995

DOE Office of Scientific and Technical Information (OSTI.GOV)

Diaz, O.

SciTech, an interactive science and technology center located 45 miles west of Chicago, is dedicated to providing hands-on and minds-on experiences that explore the spectrum of science and mathematics. SciTech opened at its present location during the summer of 1990 in Aurora, a changing community with a large minority population. Since then, SciTech has received over 331,000 visitors from the entire Chicago metropolitan area. There are now more than 250 exhibits housed in the museum. SciTech has grown quickly in part due to the strong volunteer support from the research and development corridor population it serves, as well as throughmore » international ties. SciTech has become known as an innovative force in the science museum community for its original exhibits and unique youth and school programs. SciTech`s traveling outreach program, ``Museum in a School,`` has served over 391 public and private schools in 111 districts in the Chicago metropolitan area, and has reached over 3,099 teachers and 98,837 students throughout its history. This program serves a wide variety of students, including bilingual, economically depressed, and learning impaired. SciTech is concerned with every American`s need for increasing basic science literacy.« less

Dark Secrets: What Science Tells Us About the Hidden Universe (LBNL Science at the Theater)

ScienceCinema

Permutter, Saul [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)] (ORCID:0000000244364661); Schlegel, David [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Leauthaud, Alexie [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

2018-06-12

No mystery is bigger than dark energy - the elusive force that makes up three-quarters of the Universe and is causing it to expand at an accelerating rate. KTVU Channel 2 health and science editor John Fowler will moderate a panel of Lawrence Berkeley National Laboratory scientists who use phenomena such as exploding stars and gravitational lenses to explore the dark cosmos. Saul Perlmutter heads the Supernova Cosmology Project, which pioneered the use of precise observations of exploding stars to study the expansion of the Universe. His international team was one of two groups who independently discovered the amazing phenomenon known as dark energy, and he led a collaboration that designed a satellite to study the nature of this dark force. He is an astrophysicist at Berkeley Lab and a professor of physics at UC Berkeley. David Schlegel is a Berkeley Lab astrophysicist and the principal investigator of Baryon Oscillation Spectroscopic Survey (BOSS), the largest of four night-sky surveys being conducted in the third phase of the Sloan Digital Sky Survey, known as SDSS-III. BOSS will generate a 3-D map of two million galaxies and quasars, using a specially built instrument outfitted with 1,000 optical fibers and mounted on the SDSS telescope in New Mexico. Alexie Leauthaud is Chamberlain Fellow at Berkeley Lab. Her work probes dark matter in the Universe using a technique called gravitational lensing. When gravity from a massive object such as a cluster of galaxies warps space around it, this can distort our view of the light from an even more distant object. The scale and direction of this distortion allows astronomers to directly measure the properties of both dark matter and dark energy.

The Berkeley Instrumental Neutron Generator (BINGE) for 40Ar/39Ar geochronology

NASA Astrophysics Data System (ADS)

Renne, P. R.; Becker, T. A.; Bernstein, L.; Firestone, R. B.; Kirsch, L.; Leung, K. N.; Rogers, A.; Van Bibber, K.; Waltz, C.

2014-12-01

The Berkeley Instrumental Neutron Generator (BINGE) facility is the product of a consortium involving the Berkeley Geochronology Center (BGC), the U.C. Berkeley Nuclear Engineering Dept. (UCB/NE), and Lawrence Berkeley (LBNL) and Lawrence Livermore (LLNL) National Labs. BINGE was initially designed (and funded by NSF) for 40Ar/39Ar geochronology. BINGE uses a plasma-based deuteron ion source and a self-loading Ti-surfaced target to induce deuteron-deuterium (DD) fusion via the reaction 2H(d,n)3He, producing 2.45 MeV neutrons. The limited neutron energy spectrum is aimed at reducing recoil effects, interfering nuclear reactions, and unwanted radioactive byproducts, all of which are undesirable consequences of conventional irradiation with 235U fission spectrum neutrons. Minimization of interfering reactions such as 40Ca(n,na)36Ar greatly reduces penalties for over-irradiation, enabling improved signal/background measurement of e.g. 39Ar. BINGE will also be used for a variety of nuclear physics and engineering experiments that require a high flux of monoenergetic neutrons. Neutron energies lower than 2.45 MeV can be obtained via irradiation ports within and external to polyethylene shielding. Initial commissioning produced a neutron flux of 108 n/sec/cm2 at 1 mA source current and 100 kV anode voltage, as expected. When scaled up to the 1 A source current as planned, this indicates that BINGE will achieve the design objective neutron flux of 1011 n/sec/cm2. Further progress towards this goal will be reported. Supported by NSF (grant #EAR-0960138), BGC, UCB/NE, University of California Office of the President, and DOE through LLNL under contract #DE-AC52-07NA27344 and LBNL under contract #DE-AC02-05CH11231.

Multi-sensor radiation detection, imaging, and fusion

NASA Astrophysics Data System (ADS)

Vetter, Kai

2016-01-01

Glenn Knoll was one of the leaders in the field of radiation detection and measurements and shaped this field through his outstanding scientific and technical contributions, as a teacher, his personality, and his textbook. His Radiation Detection and Measurement book guided me in my studies and is now the textbook in my classes in the Department of Nuclear Engineering at UC Berkeley. In the spirit of Glenn, I will provide an overview of our activities at the Berkeley Applied Nuclear Physics program reflecting some of the breadth of radiation detection technologies and their applications ranging from fundamental studies in physics to biomedical imaging and to nuclear security. I will conclude with a discussion of our Berkeley Radwatch and Resilient Communities activities as a result of the events at the Dai-ichi nuclear power plant in Fukushima, Japan more than 4 years ago.

Disintegration of the Aged Open Cluster Berkeley 17

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bhattacharya, Souradeep; Vaidya, Kaushar; Mishra, Ishan

We present the analysis of the morphological shape of Berkeley 17, the oldest known open cluster (∼10 Gyr), using the probabilistic star counting of Pan-STARRS point sources, and confirm its core-tail shape, plus an antitail, previously detected with the 2MASS data. The stellar population, as diagnosed by the color–magnitude diagram and theoretical isochrones, shows many massive members in the clusters core, whereas there is a paucity of such members in both of the tails. This manifests mass segregation in this aged star cluster with the low-mass members being stripped away from the system. It has been claimed that Berkeley 17more » is associated with an excessive number of blue straggler candidates. A comparison of nearby reference fields indicates that about half of these may be field contamination.« less

Does the high–tech industry consistently reduce CO{sub 2} emissions? Results from nonparametric additive regression model

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xu, Bin; Research Center of Applied Statistics, Jiangxi University of Finance and Economics, Nanchang, Jiangxi 330013; Lin, Boqiang, E-mail: bqlin@xmu.edu.cn

China is currently the world's largest carbon dioxide (CO{sub 2}) emitter. Moreover, total energy consumption and CO{sub 2} emissions in China will continue to increase due to the rapid growth of industrialization and urbanization. Therefore, vigorously developing the high–tech industry becomes an inevitable choice to reduce CO{sub 2} emissions at the moment or in the future. However, ignoring the existing nonlinear links between economic variables, most scholars use traditional linear models to explore the impact of the high–tech industry on CO{sub 2} emissions from an aggregate perspective. Few studies have focused on nonlinear relationships and regional differences in China. Basedmore » on panel data of 1998–2014, this study uses the nonparametric additive regression model to explore the nonlinear effect of the high–tech industry from a regional perspective. The estimated results show that the residual sum of squares (SSR) of the nonparametric additive regression model in the eastern, central and western regions are 0.693, 0.054 and 0.085 respectively, which are much less those that of the traditional linear regression model (3.158, 4.227 and 7.196). This verifies that the nonparametric additive regression model has a better fitting effect. Specifically, the high–tech industry produces an inverted “U–shaped” nonlinear impact on CO{sub 2} emissions in the eastern region, but a positive “U–shaped” nonlinear effect in the central and western regions. Therefore, the nonlinear impact of the high–tech industry on CO{sub 2} emissions in the three regions should be given adequate attention in developing effective abatement policies. - Highlights: • The nonlinear effect of the high–tech industry on CO{sub 2} emissions was investigated. • The high–tech industry yields an inverted “U–shaped” effect in the eastern region. • The high–tech industry has a positive “U–shaped” nonlinear effect in other regions. • The linear impact of the high–tech industry in the eastern region is the strongest.« less

A Mobile Phone Based Method to Assess Energy and Food Intake in Young Children: A Validation Study against the Doubly Labelled Water Method and 24 h Dietary Recalls

PubMed Central

Delisle Nyström, Christine; Forsum, Elisabet; Henriksson, Hanna; Trolle-Lagerros, Ylva; Larsson, Christel; Maddison, Ralph; Timpka, Toomas; Löf, Marie

2016-01-01

Mobile phones are becoming important instruments for assessing diet and energy intake. We developed the Tool for Energy Balance in Children (TECH), which uses a mobile phone to assess energy and food intake in pre-school children. The aims of this study were: (a) to compare energy intake (EI) using TECH with total energy expenditure (TEE) measured via doubly labelled water (DLW); and (b) to compare intakes of fruits, vegetables, fruit juice, sweetened beverages, candy, ice cream, and bakery products using TECH with intakes acquired by 24 h dietary recalls. Participants were 39 healthy, Swedish children (5.5 ± 0.5 years) within the ongoing Mobile-based Intervention Intended to Stop Obesity in Preschoolers (MINISTOP) obesity prevention trial. Energy and food intakes were assessed during four days using TECH and 24 h telephone dietary recalls. Mean EI (TECH) was not statistically different from TEE (DLW) (5820 ± 820 kJ/24 h and 6040 ± 680kJ/24 h, respectively). No significant differences in the average food intakes using TECH and 24 h dietary recalls were found. All food intakes were correlated between TECH and the 24 h dietary recalls (ρ = 0.665–0.896, p < 0.001). In conclusion, TECH accurately estimated the average intakes of energy and selected foods and thus has the potential to be a useful tool for dietary studies in pre-school children, for example obesity prevention trials. PMID:26784226

Map showing bottom topography of the Pacific Continental Margin, Strait of Juan de Fuca to Cape Mendocino

USGS Publications Warehouse

Grim, M.S.; Chase, T.E.; Evenden, G.I.; Holmes, M.L.; Normark, W.R.; Wilde, Pat; Fox, C.J.; Lief, C.J.; Seekins, B.A.

1992-01-01

Wilde, Pat, Chase, T.E., Holmes, M.L., Normark, W.R., Thomas, J.A., McCulloch, D.S., and Kulm, L.D., 1978, Oceanographic data off northern California-southern Oregon 40° to 43° North including the Gorda Deep Sea Fan: Berkeley, University of California, Lawrence Berkeley Laboratory Publication 251, scale 1:815,482 at 42° latitude.

Collection Conservation Treatment: A Resource Manual for Program Development and Conservation Technician Training, Including "Report on Training the Trainers: A Conference on Training in Collection Conservation, Berkeley, California, April 28-May 2, 1992."

ERIC Educational Resources Information Center

Jones, Maralyn, Comp.

The documentation of collection conservation treatments and program management information compiled in this publication are the result of the "Training the Trainers" conference in April 1992 at the University of California, Berkeley. Only treatments appropriate for general, usually circulating, collections have been included, emphasizing…

What is Gravitational Lensing?(LBNL Summer Lecture Series)

ScienceCinema

Alexie, Leauthaud; Reiko, Nakajima [Berkeley Center for Cosmological Physics, Berkely, California, United States

2017-12-09

July 28, 2009 Berkeley Lab summer lecture: Gravitational lensing is explained by Einstein's general theory of relativity: galaxies and clusters of galaxies, which are very massive objects, act on spacetime by causing it to become curved. Alexie Leauthaud and Reiko Nakajima, astrophysicists with the Berkeley Center for Cosmological Physics, will discuss how scientists use gravitational lensing to investigate the nature of dark energy and dark matter in the universe.

First Light for BOSS - A New Kind of Search for Dark Energy | Berkeley

Science.gov Websites

in the clumping of invisible dark matter. Comparing these scales at different eras makes it possible Web People Close About the Lab Leadership/Organization Calendar News Center Today At Berkeley Lab News Preuss, (510) 486-6249 On the night of September 14 the largest program in the Sloan Digital Sky Survey

Nanoscience at Work: Creating Energy from Sunlight (LBNL Science at the Theater)

ScienceCinema

Alivisatos, Paul [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

2018-02-26

Paul Alivisatos, co-leader of Berkeley Lab's Helios Project, is the Associate Director for Physical Sciences and director of the Materials Sciences Division at Berkeley Lab. In the Helios Project, Alivisatos will use nanotechnology in the efficient capture of sunlight and its conversion to electricity to drive economical fuel production processes. He is an authority on artificial nanostructure synthesis and inventor of the quantum dot technology.

Berkeley's New Approach to Global Engagement: Early and Current Efforts to Become More International. Research & Occasional Paper Series: CSHE.12.15

ERIC Educational Resources Information Center

Dirks, Nicholas B.; Gilman, Nils

2015-01-01

This essay discusses past and current thinking about the globalization of higher education (from a U.S. point of view in particular) and a new model we are attempting to develop at the University of California, Berkeley. This essay begins with a brief narrative of the historical evolution of efforts to internationalize education, from the…

Expanding Off-Campus Enrollment Capacity at Berkeley: A Concept Paper. Research & Occasional Paper Series: CSHE.2.17

ERIC Educational Resources Information Center

Geiser, Saul

2017-01-01

Like Berkeley, the UC system as a whole is quickly running out of space to accommodate the next generation of Californians who will be reaching college age by mid-century. Even with the added capacity at UC Merced, the UC system will run out of space on existing campuses in the next decade. In the normal course of events, this would trigger…

Better Batteries for Transportation: Behind the Scenes @ Berkeley Lab

DOE Office of Scientific and Technical Information (OSTI.GOV)

Battaglia, Vince

Vince Battaglia leads a behind-the-scenes tour of Berkeley Lab's BATT, the Batteries for Advanced Transportation Technologies Program he leads, where researchers aim to improve batteries upon which the range, efficiency, and power of tomorrow's electric cars will depend. This is the first in a forthcoming series of videos taking viewers into the laboratories and research facilities that members of the public rarely get to see.

POWER AND AUTHORITY--EMERGING TRENDS AND ASPIRATIONS, PROCEEDINGS OF THE STANFORD-BERKELEY SEMINAR FOR JUNIOR COLLEGE PRESIDENTS (2D, BERKELEY, JUNE 19-21, 1964).

ERIC Educational Resources Information Center

MEDSKER, LELAND L.; TILLERY, HARRY DALE

FIVE PAPERS ARE REPRODUCED IN THIS REPORT. DR. DONALD D. JACKSON DISCUSSED THE CHANGING POWER STRUCTURE IN MODERN SOCIETIES, AS PREPARATION FOR THE EXAMINATION OF TRENDS RELEVANT TO CRUCIAL ASPECTS OF THE COLLEGE COMMUNITY. GORDON W. BLACKWELL DESCRIBED THE FORMAL POWER STRUCTURE, THE INFORMAL POWER STRUCTURE, AND AREAS OF TENSION IN STATE…

PART 2 OF 2 PART PANORAMA WITH NO. CA212 VIEW ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PART 2 OF 2 PART PANORAMA WITH NO. CA-2-12 VIEW OF PIEDMONT AVENUE TRAFFIC CIRCLE AT INTERSECTION OF CHANNING WAY WITH 2395 PIEDMONT, SIGMA PI HOUSE BY FREDERICK H. REIMERS, 1928. SEEN FROM SE CORNER LOOKING NW. Photograph by Brian Grogan, July 8, 2007 - Piedmont Way & the Berkeley Property Tract, East of College Avenue between Dwight Way & U.C. Memorial Stadium, Berkeley, Alameda County, CA

How Bilayer Graphene Got a Bandgap

DOE Office of Scientific and Technical Information (OSTI.GOV)

Feng Wang

2009-06-02

Graphene is the two-dimensional crystalline form of carbon, whose extraordinary electron mobility and other unique features hold great promise for nanoscale electronics and photonics. But theres a catch: graphene has no bandgap. Now Feng Wang and his colleagues at Berkeley Lab and UC Berkeley have engineered a bandgap in bilayer graphene that can be precisely controlled from 0 to 250 milli-electron volts, which is the energy of infrared radiation.

Better Batteries for Transportation: Behind the Scenes @ Berkeley Lab

ScienceCinema

Battaglia, Vince

2018-02-06

Vince Battaglia leads a behind-the-scenes tour of Berkeley Lab's BATT, the Batteries for Advanced Transportation Technologies Program he leads, where researchers aim to improve batteries upon which the range, efficiency, and power of tomorrow's electric cars will depend. This is the first in a forthcoming series of videos taking viewers into the laboratories and research facilities that members of the public rarely get to see.

Progress Report on the Berkeley/Anglo-Australian Observatory High-redshift Supernova Search

DOE R&D Accomplishments Database

Goldhaber, G.; Perlmutter, S.; Pennypacker, C.; Marvin, H.; Muller, R. A.; Couch, W.; Boyle, B.

1990-11-01

There are two main efforts related to supernovae in progress at Berkeley. The first is an automated supernova search for nearby supernovae, which was already discussed by Carl Pennypacker at this conference. The second is a search for distant supernovae, in the z = 0.3 to 0.5 region, aimed at measuring {Omega}. It is the latter that I want to discuss in this paper.

How Bilayer Graphene Got a Bandgap

ScienceCinema

Feng Wang

2017-12-09

Graphene is the two-dimensional crystalline form of carbon, whose extraordinary electron mobility and other unique features hold great promise for nanoscale electronics and photonics. But theres a catch: graphene has no bandgap. Now Feng Wang and his colleagues at Berkeley Lab and UC Berkeley have engineered a bandgap in bilayer graphene that can be precisely controlled from 0 to 250 milli-electron volts, which is the energy of infrared radiation.

How Bilayer Graphene Got a Bandgap

ScienceCinema

Wang, Feng

2018-01-08

Graphene is the two-dimensional crystalline form of carbon, whose extraordinary electron mobility and other unique features hold great promise for nanoscale electronics and photonics. But theres a catch: graphene has no bandgap. Now Feng Wang and his colleagues at Berkeley Lab and UC Berkeley have engineered a bandgap in bilayer graphene that can be precisely controlled from 0 to 250 milli-electron volts, which is the energy of infrared radiation.

Laboratory directed research and development program, FY 1996

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1997-02-01

The Ernest Orlando Lawrence Berkeley National Laboratory (Berkeley Lab) Laboratory Directed Research and Development Program FY 1996 report is compiled from annual reports submitted by principal investigators following the close of the fiscal year. This report describes the projects supported and summarizes their accomplishments. It constitutes a part of the Laboratory Directed Research and Development (LDRD) program planning and documentation process that includes an annual planning cycle, projection selection, implementation, and review. The Berkeley Lab LDRD program is a critical tool for directing the Laboratory`s forefront scientific research capabilities toward vital, excellent, and emerging scientific challenges. The program provides themore » resources for Berkeley Lab scientists to make rapid and significant contributions to critical national science and technology problems. The LDRD program also advances the Laboratory`s core competencies, foundations, and scientific capability, and permits exploration of exciting new opportunities. Areas eligible for support include: (1) Work in forefront areas of science and technology that enrich Laboratory research and development capability; (2) Advanced study of new hypotheses, new experiments, and innovative approaches to develop new concepts or knowledge; (3) Experiments directed toward proof of principle for initial hypothesis testing or verification; and (4) Conception and preliminary technical analysis to explore possible instrumentation, experimental facilities, or new devices.« less

Economic impact

DOE Office of Scientific and Technical Information (OSTI.GOV)

Technology Transfer Department

2001-06-01

In federal fiscal year 2000 (FY00), Berkeley Lab had 4,347 full- and part-time employees. In addition, at any given time of the year, there were more than 1,000 Laboratory guests. These guests, who also reside locally, have an important economic impact on the nine-county Bay Area. However, Berkeley Lab's total economic impact transcends the direct effects of payroll and purchasing. The direct dollars paid to the Lab's employees in the form of wages, salaries, and benefits, and payments made to contractors for goods and services, are respent by employees and contractors again and again in the local and greater economy.more » Further, while Berkeley Lab has a strong reputation for basic scientific research, many of the Lab's scientific discoveries and inventions have had direct application in industry, spawning new businesses and creating new opportunities for existing firms. This analysis updates the Economic Impact Analysis done in 1996, and its purpose is to describe the economic and geographic impact of Laboratory expenditures and to provide a qualitative understanding of how Berkeley Lab impacts and supports the local community. It is intended as a guide for state, local, and national policy makers as well as local community members. Unless otherwise noted, this analysis uses data from FY00, the most recent year for which full data are available.« less

Virginia Tech Researchers Study Effects of Shootings on Their Campus

ERIC Educational Resources Information Center

Gravois, John

2008-01-01

This article reports how researchers at Virginia Tech, working in a variety of disciplines, are studying the long-term effects of the campus killings 10 months ago. After the killings, administrators at Virginia Tech realized that their normal institutional-review process for research proposals was not sufficient to deal with the likely onslaught…