Nutrient Sensing Systems in Fish: Impact on Food Intake Regulation and Energy Homeostasis

PubMed Central

Conde-Sieira, Marta; Soengas, José L.

2017-01-01

Evidence obtained in recent years in a few species, especially rainbow trout, supports the presence in fish of nutrient sensing mechanisms. Glucosensing capacity is present in central (hypothalamus and hindbrain) and peripheral [liver, Brockmann bodies (BB, main accumulation of pancreatic endocrine cells in several fish species), and intestine] locations whereas fatty acid sensors seem to be present in hypothalamus, liver and BB. Glucose and fatty acid sensing capacities relate to food intake regulation and metabolism in fish. Hypothalamus is as a signaling integratory center in a way that detection of increased levels of nutrients result in food intake inhibition through changes in the expression of anorexigenic and orexigenic neuropeptides. Moreover, central nutrient sensing modulates functions in the periphery since they elicit changes in hepatic metabolism as well as in hormone secretion to counter-regulate changes in nutrient levels detected in the CNS. At peripheral level, the direct nutrient detection in liver has a crucial role in homeostatic control of glucose and fatty acid whereas in BB and intestine nutrient sensing is probably involved in regulation of hormone secretion from endocrine cells. PMID:28111540

Nutrient sensing and signaling in the yeast Saccharomyces cerevisiae

PubMed Central

Conrad, Michaela; Schothorst, Joep; Kankipati, Harish Nag; Van Zeebroeck, Griet; Rubio-Texeira, Marta; Thevelein, Johan M

2014-01-01

The yeast Saccharomyces cerevisiae has been a favorite organism for pioneering studies on nutrient-sensing and signaling mechanisms. Many specific nutrient responses have been elucidated in great detail. This has led to important new concepts and insight into nutrient-controlled cellular regulation. Major highlights include the central role of the Snf1 protein kinase in the glucose repression pathway, galactose induction, the discovery of a G-protein-coupled receptor system, and role of Ras in glucose-induced cAMP signaling, the role of the protein synthesis initiation machinery in general control of nitrogen metabolism, the cyclin-controlled protein kinase Pho85 in phosphate regulation, nitrogen catabolite repression and the nitrogen-sensing target of rapamycin pathway, and the discovery of transporter-like proteins acting as nutrient sensors. In addition, a number of cellular targets, like carbohydrate stores, stress tolerance, and ribosomal gene expression, are controlled by the presence of multiple nutrients. The protein kinase A signaling pathway plays a major role in this general nutrient response. It has led to the discovery of nutrient transceptors (transporter receptors) as nutrient sensors. Major shortcomings in our knowledge are the relationship between rapid and steady-state nutrient signaling, the role of metabolic intermediates in intracellular nutrient sensing, and the identity of the nutrient sensors controlling cellular growth. PMID:24483210

Primary Respiratory Chain Disease Causes Tissue-Specific Dysregulation of the Global Transcriptome and Nutrient-Sensing Signaling Network

PubMed Central

Zhang, Zhe; Tsukikawa, Mai; Peng, Min; Polyak, Erzsebet; Nakamaru-Ogiso, Eiko; Ostrovsky, Julian; McCormack, Shana; Place, Emily; Clarke, Colleen; Reiner, Gail; McCormick, Elizabeth; Rappaport, Eric; Haas, Richard; Baur, Joseph A.; Falk, Marni J.

2013-01-01

Primary mitochondrial respiratory chain (RC) diseases are heterogeneous in etiology and manifestations but collectively impair cellular energy metabolism. Mechanism(s) by which RC dysfunction causes global cellular sequelae are poorly understood. To identify a common cellular response to RC disease, integrated gene, pathway, and systems biology analyses were performed in human primary RC disease skeletal muscle and fibroblast transcriptomes. Significant changes were evident in muscle across diverse RC complex and genetic etiologies that were consistent with prior reports in other primary RC disease models and involved dysregulation of genes involved in RNA processing, protein translation, transport, and degradation, and muscle structure. Global transcriptional and post-transcriptional dysregulation was also found to occur in a highly tissue-specific fashion. In particular, RC disease muscle had decreased transcription of cytosolic ribosomal proteins suggestive of reduced anabolic processes, increased transcription of mitochondrial ribosomal proteins, shorter 5′-UTRs that likely improve translational efficiency, and stabilization of 3′-UTRs containing AU-rich elements. RC disease fibroblasts showed a strikingly similar pattern of global transcriptome dysregulation in a reverse direction. In parallel with these transcriptional effects, RC disease dysregulated the integrated nutrient-sensing signaling network involving FOXO, PPAR, sirtuins, AMPK, and mTORC1, which collectively sense nutrient availability and regulate cellular growth. Altered activities of central nodes in the nutrient-sensing signaling network were validated by phosphokinase immunoblot analysis in RC inhibited cells. Remarkably, treating RC mutant fibroblasts with nicotinic acid to enhance sirtuin and PPAR activity also normalized mTORC1 and AMPK signaling, restored NADH/NAD+ redox balance, and improved cellular respiratory capacity. These data specifically highlight a common pathogenesis extending across different molecular and biochemical etiologies of individual RC disorders that involves global transcriptome modifications. We further identify the integrated nutrient-sensing signaling network as a common cellular response that mediates, and may be amenable to targeted therapies for, tissue-specific sequelae of primary mitochondrial RC disease. PMID:23894440

Influence of Nutrient Availability and Quorum Sensing on the Formation of Metabolically Inactive Microcolonies Within Structurally Heterogeneous Bacterial Biofilms: An Individual-Based 3D Cellular Automata Model.

PubMed

Machineni, Lakshmi; Rajapantul, Anil; Nandamuri, Vandana; Pawar, Parag D

2017-03-01

The resistance of bacterial biofilms to antibiotic treatment has been attributed to the emergence of structurally heterogeneous microenvironments containing metabolically inactive cell populations. In this study, we use a three-dimensional individual-based cellular automata model to investigate the influence of nutrient availability and quorum sensing on microbial heterogeneity in growing biofilms. Mature biofilms exhibited at least three structurally distinct strata: a high-volume, homogeneous region sandwiched between two compact sections of high heterogeneity. Cell death occurred preferentially in layers in close proximity to the substratum, resulting in increased heterogeneity in this section of the biofilm; the thickness and heterogeneity of this lowermost layer increased with time, ultimately leading to sloughing. The model predicted the formation of metabolically dormant cellular microniches embedded within faster-growing cell clusters. Biofilms utilizing quorum sensing were more heterogeneous compared to their non-quorum sensing counterparts, and resisted sloughing, featuring a cell-devoid layer of EPS atop the substratum upon which the remainder of the biofilm developed. Overall, our study provides a computational framework to analyze metabolic diversity and heterogeneity of biofilm-associated microorganisms and may pave the way toward gaining further insights into the biophysical mechanisms of antibiotic resistance.

Neuronal regulation of homeostasis by nutrient sensing.

PubMed

Lam, Tony K T

2010-04-01

In type 2 diabetes and obesity, the homeostatic control of glucose and energy balance is impaired, leading to hyperglycemia and hyperphagia. Recent studies indicate that nutrient-sensing mechanisms in the body activate negative-feedback systems to regulate energy and glucose homeostasis through a neuronal network. Direct metabolic signaling within the intestine activates gut-brain and gut-brain-liver axes to regulate energy and glucose homeostasis, respectively. In parallel, direct metabolism of nutrients within the hypothalamus regulates food intake and blood glucose levels. These findings highlight the importance of the central nervous system in mediating the ability of nutrient sensing to maintain homeostasis. Futhermore, they provide a physiological and neuronal framework by which enhancing or restoring nutrient sensing in the intestine and the brain could normalize energy and glucose homeostasis in diabetes and obesity.

Remote Sensing Applications to Water Quality Management in Florida

NASA Astrophysics Data System (ADS)

Lehrter, J. C.; Schaeffer, B. A.; Hagy, J.; Spiering, B.; Barnes, B.; Hu, C.; Le, C.; McEachron, L.; Underwood, L. W.; Ellis, C.; Fisher, B.

2013-12-01

Optical datasets from estuarine and coastal systems are increasingly available for remote sensing algorithm development, validation, and application. With validated algorithms, the data streams from satellite sensors can provide unprecedented spatial and temporal data for local and regional coastal water quality management. Our presentation will highlight two recent applications of optical data and remote sensing to water quality decision-making in coastal regions of the state of Florida; (1) informing the development of estuarine and coastal nutrient criteria for the state of Florida and (2) informing the rezoning of the Florida Keys National Marine Sanctuary. These efforts involved building up the underlying science to demonstrate the applicability of satellite data as well as an outreach component to educate decision-makers about the use, utility, and uncertainties of remote sensing data products. Scientific developments included testing existing algorithms and generating new algorithms for water clarity and chlorophylla in case II (CDOM or turbidity dominated) estuarine and coastal waters and demonstrating the accuracy of remote sensing data products in comparison to traditional field based measurements. Including members from decision-making organizations on the research team and interacting with decision-makers early and often in the process were key factors for the success of the outreach efforts and the eventual adoption of satellite data into the data records and analyses used in decision-making. Florida coastal water bodies (black boxes) for which remote sensing imagery were applied to derive numeric nutrient criteria and in situ observations (black dots) used to validate imagery. Florida ocean color applied to development of numeric nutrient criteria

Molecular pharmacology of promiscuous seven transmembrane receptors sensing organic nutrients.

PubMed

Wellendorph, Petrine; Johansen, Lars Dan; Bräuner-Osborne, Hans

2009-09-01

A number of highly promiscuous seven transmembrane (7TM) receptors have been cloned and characterized within the last few years. It is noteworthy that many of these receptors are activated broadly by amino acids, proteolytic degradation products, carbohydrates, or free fatty acids and are expressed in taste tissue, the gastrointestinal tract, endocrine glands, adipose tissue, and/or kidney. These receptors thus hold the potential to act as sensors of food intake, regulating, for example, release of incretin hormones from the gut, insulin/glucagon from the pancreas, and leptin from adipose tissue. The promiscuous tendency in ligand recognition of these receptors is in contrast to the typical specific interaction with one physiological agonist seen for most receptors, which challenges the classic "lock-and-key" concept. We here review the molecular mechanisms of nutrient sensing of the calcium-sensing receptor, the G protein-coupled receptor family C, group 6, subtype A (GPRC6A), and the taste1 receptor T1R1/T1R3, which are sensing L-alpha-amino acids, the carbohydrate-sensing T1R2/T1R3 receptor, the proteolytic degradation product sensor GPR93 (also termed GPR92), and the free fatty acid (FFA) sensing receptors FFA1, FFA2, FFA3, GPR84, and GPR120. The involvement of the individual receptors in sensing of food intake has been validated to different degrees because of limited availability of specific pharmacological tools and/or receptor knockout mice. However, as a group, the receptors represent potential drug targets, to treat, for example, type II diabetes by mimicking food intake by potent agonists or positive allosteric modulators. The ligand-receptor interactions of the promiscuous receptors of organic nutrients thus remain an interesting subject of emerging functional importance.

Amino acid–insensitive mTORC1 regulation enables nutritional stress resilience in hematopoietic stem cells

PubMed Central

Kalaitzidis, Demetrios; Efeyan, Alejo; Kfoury, Youmna; Nayyar, Naema; Sykes, David B.; Mercier, Francois E.; Papazian, Ani; Baryawno, Ninib; Victora, Gabriel D.; Sabatini, David M.; Scadden, David T.

2017-01-01

The mTOR pathway is a critical determinant of cell persistence and growth wherein mTOR complex 1 (mTORC1) mediates a balance between growth factor stimuli and nutrient availability. Amino acids or glucose facilitates mTORC1 activation by inducing RagA GTPase recruitment of mTORC1 to the lysosomal outer surface, enabling activation of mTOR by the Ras homolog Rheb. Thereby, RagA alters mTORC1-driven growth in times of nutrient abundance or scarcity. Here, we have evaluated differential nutrient-sensing dependence through RagA and mTORC1 in hematopoietic progenitors, which dynamically drive mature cell production, and hematopoietic stem cells (HSC), which provide a quiescent cellular reserve. In nutrient-abundant conditions, RagA-deficient HSC were functionally unimpaired and upregulated mTORC1 via nutrient-insensitive mechanisms. RagA was also dispensable for HSC function under nutritional stress conditions. Similarly, hyperactivation of RagA did not affect HSC function. In contrast, RagA deficiency markedly altered progenitor population function and mature cell output. Therefore, RagA is a molecular mechanism that distinguishes the functional attributes of reactive progenitors from a reserve stem cell pool. The indifference of HSC to nutrient sensing through RagA contributes to their molecular resilience to nutritional stress, a characteristic that is relevant to organismal viability in evolution and in modern HSC transplantation approaches. PMID:28319048

The islet beta-cell: fuel responsive and vulnerable.

PubMed

Nolan, Christopher J; Prentki, Marc

2008-10-01

The pancreatic beta-cell senses blood nutrient levels and is modulated by neurohormonal signals so that it secretes insulin according to the need of the organism. Nutrient sensing involves marked metabolic activation, resulting in the production of coupling signals that promote insulin biosynthesis and secretion. The beta-cell's high capacity for nutrient sensing, however, necessitates reduced protection to nutrient toxicity. This potentially explains why in susceptible individuals, chronic fuel surfeit results in beta-cell failure and type 2 diabetes. Here we discuss recent insights into first, the biochemical basis of beta-cell signaling in response to glucose, amino acids and fatty acids, and second, beta-cell nutrient detoxification. We emphasize the emerging role of glycerolipid/fatty acid cycling in these processes.

Cellular Oxygen and Nutrient Sensing in Microgravity Using Time-Resolved Fluorescence Microscopy

NASA Technical Reports Server (NTRS)

Szmacinski, Henryk

2003-01-01

Oxygen and nutrient sensing is fundamental to the understanding of cell growth and metabolism. This requires identification of optical probes and suitable detection technology without complex calibration procedures. Under this project Microcosm developed an experimental technique that allows for simultaneous imaging of intra- and inter-cellular events. The technique consists of frequency-domain Fluorescence Lifetime Imaging Microscopy (FLIM), a set of identified oxygen and pH probes, and methods for fabrication of microsensors. Specifications for electronic and optical components of FLIM instrumentation are provided. Hardware and software were developed for data acquisition and analysis. Principles, procedures, and representative images are demonstrated. Suitable lifetime sensitive oxygen, pH, and glucose probes for intra- and extra-cellular measurements of analyte concentrations have been identified and tested. Lifetime sensing and imaging have been performed using PBS buffer, culture media, and yeast cells as a model systems. Spectral specifications, calibration curves, and probes availability are also provided in the report.

The Role of Placental Nutrient Sensing in Maternal-Fetal Resource Allocation1

PubMed Central

Díaz, Paula; Powell, Theresa L.; Jansson, Thomas

2014-01-01

ABSTRACT The placenta mediates maternal-fetal exchange and has historically been regarded as a passive conduit for nutrients. However, emerging evidence suggests that the placenta actively responds to nutritional and metabolic signals from the mother and the fetus. We propose that the placenta integrates a multitude of maternal and fetal nutritional cues with information from intrinsic nutrient-sensing signaling pathways to match fetal demand with maternal supply by regulating maternal physiology, placental growth, and nutrient transport. This process, which we have called placental nutrient sensing, ensures optimal allocation of resources between the mother and the fetus to maximize the chances for propagation of parental genes without jeopardizing maternal health. We suggest that these mechanisms have evolved because of the evolutionary pressures of maternal undernutrition, which result in decreased placental growth and down-regulation of nutrient transporters, thereby limiting fetal growth to ensure maternal survival. These regulatory loops may also function in response to maternal overnutrition, leading to increased placental growth and nutrient transport in cases of maternal obesity or gestational diabetes. Thus, placental nutrient sensing modulates maternal-fetal resource allocation to increase the likelihood of reproductive success. This model implies that the placenta plays a critical role in mediating fetal programming and determining lifelong health. PMID:25122064

Small heterodimer partner (NROB2) coordinates nutrient signaling and the circadian clock in mice

USDA-ARS?s Scientific Manuscript database

Circadian rhythm regulates multiple metabolic processes and in turn is readily entrained by feeding-fasting cycles. However, the molecular mechanisms by which the peripheral clock senses nutrition availability remain largely unknown. Bile acids are under circadian control and also increase postprand...

Nutrient Sensing at the Plasma Membrane of Fungal Cells.

PubMed

Van Dijck, Patrick; Brown, Neil Andrew; Goldman, Gustavo H; Rutherford, Julian; Xue, Chaoyang; Van Zeebroeck, Griet

2017-03-01

To respond to the changing environment, cells must be able to sense external conditions. This is important for many processes including growth, mating, the expression of virulence factors, and several other regulatory effects. Nutrient sensing at the plasma membrane is mediated by different classes of membrane proteins that activate downstream signaling pathways: nontransporting receptors, transceptors, classical and nonclassical G-protein-coupled receptors, and the newly defined extracellular mucin receptors. Nontransporting receptors have the same structure as transport proteins, but have lost the capacity to transport while gaining a receptor function. Transceptors are transporters that also function as a receptor, because they can rapidly activate downstream signaling pathways. In this review, we focus on these four types of fungal membrane proteins. We mainly discuss the sensing mechanisms relating to sugars, ammonium, and amino acids. Mechanisms for other nutrients, such as phosphate and sulfate, are discussed briefly. Because the model yeast Saccharomyces cerevisiae has been the most studied, especially regarding these nutrient-sensing systems, each subsection will commence with what is known in this species.

Lysosomal Physiology

PubMed Central

Xu, Haoxing; Ren, Dejian

2015-01-01

Lysosomes are acidic compartments filled with more than 60 different types of hydrolases. They mediate the degradation of extracellular particles from endocytosis and of intracellular components from autophagy. The digested products are transported out of the lysosome via specific catabolite exporters or via vesicular membrane trafficking. Lysosomes also contain more than 50 membrane proteins and are equipped with the machinery to sense nutrient availability, which determines the distribution, number, size, and activity of lysosomes to control the specificity of cargo flux and timing (the initiation and termination) of degradation. Defects in degradation, export, or trafficking result in lysosomal dysfunction and lysosomal storage diseases (LSDs). Lysosomal channels and transporters mediate ion flux across perimeter membranes to regulate lysosomal ion homeostasis, membrane potential, catabolite export, membrane trafficking, and nutrient sensing. Dysregulation of lysosomal channels underlies the pathogenesis of many LSDs and possibly that of metabolic and common neurodegenerative diseases. PMID:25668017

Hypothalamic nutrient sensing activates a forebrain-hindbrain neuronal circuit to regulate glucose production in vivo.

PubMed

Lam, Carol K L; Chari, Madhu; Rutter, Guy A; Lam, Tony K T

2011-01-01

Hypothalamic nutrient sensing regulates glucose production, but the neuronal circuits involved remain largely unknown. Recent studies underscore the importance of N-methyl-d-aspartate (NMDA) receptors in the dorsal vagal complex in glucose regulation. These studies raise the possibility that hypothalamic nutrient sensing activates a forebrain-hindbrain NMDA-dependent circuit to regulate glucose production. We implanted bilateral catheters targeting the mediobasal hypothalamus (MBH) (forebrain) and dorsal vagal complex (DVC) (hindbrain) and performed intravenous catheterizations to the same rat for infusion and sampling purposes. This model enabled concurrent selective activation of MBH nutrient sensing by either MBH delivery of lactate or an adenovirus expressing the dominant negative form of AMPK (Ad-DN AMPK α2 [D¹⁵⁷A]) and inhibition of DVC NMDA receptors by either DVC delivery of NMDA receptor blocker MK-801 or an adenovirus expressing the shRNA of NR1 subunit of NMDA receptors (Ad-shRNA NR1). Tracer-dilution methodology and the pancreatic euglycemic clamp technique were performed to assess changes in glucose kinetics in the same conscious, unrestrained rat in vivo. MBH lactate or Ad-DN AMPK with DVC saline increased glucose infusion required to maintain euglycemia due to an inhibition of glucose production during the clamps. However, DVC MK-801 negated the ability of MBH lactate or Ad-DN AMPK to increase glucose infusion or lower glucose production. Molecular knockdown of DVC NR1 of NMDA receptor via Ad-shRNA NR1 injection also negated MBH Ad-DN AMPK to lower glucose production. Molecular and pharmacological inhibition of DVC NMDA receptors negated hypothalamic nutrient sensing mechanisms activated by lactate metabolism or AMPK inhibition to lower glucose production. Thus, DVC NMDA receptor is required for hypothalamic nutrient sensing to lower glucose production and that hypothalamic nutrient sensing activates a forebrain-hindbrain circuit to lower glucose production.

A Two-Layer Gene Circuit for Decoupling Cell Growth from Metabolite Production.

PubMed

Lo, Tat-Ming; Chng, Si Hui; Teo, Wei Suong; Cho, Han-Saem; Chang, Matthew Wook

2016-08-01

We present a synthetic gene circuit for decoupling cell growth from metabolite production through autonomous regulation of enzymatic pathways by integrated modules that sense nutrient and substrate. The two-layer circuit allows Escherichia coli to selectively utilize target substrates in a mixed pool; channel metabolic resources to growth by delaying enzymatic conversion until nutrient depletion; and activate, terminate, and re-activate conversion upon substrate availability. We developed two versions of controller, both of which have glucose nutrient sensors but differ in their substrate-sensing modules. One controller is specific for hydroxycinnamic acid and the other for oleic acid. Our hydroxycinnamic acid controller lowered metabolic stress 2-fold and increased the growth rate 2-fold and productivity 5-fold, whereas our oleic acid controller lowered metabolic stress 2-fold and increased the growth rate 1.3-fold and productivity 2.4-fold. These results demonstrate the potential for engineering strategies that decouple growth and production to make bio-based production more economical and sustainable. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Nutrient Regulation of the mTOR Complex 1 Signaling Pathway

PubMed Central

Kim, Sang Gyun; Buel, Gwen R.; Blenis, John

2013-01-01

The mammalian target of rapamycin (mTOR) is an evolutionally conserved kinase which exists in two distinct structural and functional complexes, mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). Of the two complexes, mTORC1 couples nutrient abundance to cell growth and proliferation by sensing and integrating a variety of inputs arising from amino acids, cellular stresses, energy status, and growth factors. Defects in mTORC1 regulation are implicated in the development of many metabolic diseases, including cancer and diabetes. Over the past decade, significant advances have been made in deciphering the complexity of the signaling processes contributing to mTORC1 regulation and function, but the mechanistic details are still not fully understood. In particular, how amino acid availability is sensed by cells and signals to mTORC1 remains unclear. In this review, we discuss the current understanding of nutrient-dependent control of mTORC1 signaling and will focus on the key components involved in amino acid signaling to mTORC1. PMID:23694989

Recent developments in sensing methods for eutrophying nutrients with a focus on automation for environmental applications.

PubMed

Duffy, G; Regan, F

2017-11-20

The demand for autonomous sensors for unattended, continuous nutrient monitoring in water is rapidly growing with the increasing need for more frequent and widespread environmental pollution monitoring. Legislative bodies, local authorities and industries all require frequent water quality monitoring, however, this is time and labour intensive, and an expensive undertaking. Autonomous sensors allow for frequent, unattended data collection. While this solves the time and labour intensive aspects of water monitoring, sensors can be very expensive. Development of low-cost sensors is essential to realise the concept of Internet of Things (IoT). However there is much work yet to be done in this field. This article reviews current literature on the research and development efforts towards deployable autonomous sensors for phosphorus (in the form of phosphate) and nitrogen (in the form of nitrate), with a focus on analytical performance and cost considerations. Additionally, some recent sensing approaches that could be automated in the future are included, along with an overview of approaches to monitoring both nutrients. These approaches are compared with standard laboratory methods and also with commercially available sensors for both phosphate and nitrate. Application of nutrient sensors in agriculture is discussed as an example of how sensor networks can provide improvements in decision making.

Nutritional Applications of the Chemical Senses.

ERIC Educational Resources Information Center

Naim, Michael; Kare, Morley R.

1984-01-01

Discusses the relationship of taste and smell to ingestion, digestion, and metabolism. Indicates that the response of these physiological systems can be chemical specific and that chemical senses may play different roles in regulating diet during nutrient deficiency and during nutrient surplus situations. (JN)

Nutrient sensing in plant meristems.

PubMed

Francis, Dennis; Halford, Nigel G

2006-04-01

Plants need nutrient to grow and plant cells need nutrient to divide. The meristems are the factories and cells that are left behind will expand and differentiate. However, meristems are not simple homogenous entities; cells in different parts of the meristem do different things. Positional cues operate that can fate cells into different tissue domains. However, founder/stem cells persist in specific locations within the meristem e.g. the quiescent centre of root apical meristem (RAM) and the lower half of the central zone of the shoot apical meristem (SAM). Given the complexity of meristems, do their cells simply respond to a diffusing gradient of photosynthate? This in turn begs the question, why do stem cell populations tend to have longer cell cycles than their immediate descendants given that like all other cells they are directly in the path of diffusing nutrient? In this review, we have examined the extent to which nutrient sensing might be operating in meristems. The scene is set for sugar sensing, the plant cell cycle, SAMs and RAMs. Special emphasis is given to the metabolic regulator, SnRK1 (SNF1-related protein kinase 1), hexokinase and the trehalose pathway in relation to sugar sensing. The unique plant cell cycle gene, cyclin-dependent kinase B1;1 may have evolved to be particularly responsive to sugar signalling pathways. Also, the homeobox gene, STIMPY, emerges strongly as a link between sugar sensing, plant cell proliferation and development. Flowering can be influenced by sucrose and glucose levels and both meristem identity and organ identity genes could well be differentially sensitive to sucrose and glucose signals. We also describe how meristems deal with extra photosynthate as a result of exposure to elevated CO2. What we review are numerous instances of how developmental processes can be affected by sugars/nutrients. However, given the scarcity of knowledge we are unable to provide uncontested links between nutrient sensing and specific activities in meristems.

Hypothalamic carnitine metabolism integrates nutrient and hormonal feedback to regulate energy homeostasis.

PubMed

Stark, Romana; Reichenbach, Alex; Andrews, Zane B

2015-12-15

The maintenance of energy homeostasis requires the hypothalamic integration of nutrient feedback cues, such as glucose, fatty acids, amino acids, and metabolic hormones such as insulin, leptin and ghrelin. Although hypothalamic neurons are critical to maintain energy homeostasis research efforts have focused on feedback mechanisms in isolation, such as glucose alone, fatty acids alone or single hormones. However this seems rather too simplistic considering the range of nutrient and endocrine changes associated with different metabolic states, such as starvation (negative energy balance) or diet-induced obesity (positive energy balance). In order to understand how neurons integrate multiple nutrient or hormonal signals, we need to identify and examine potential intracellular convergence points or common molecular targets that have the ability to sense glucose, fatty acids, amino acids and hormones. In this review, we focus on the role of carnitine metabolism in neurons regulating energy homeostasis. Hypothalamic carnitine metabolism represents a novel means for neurons to facilitate and control both nutrient and hormonal feedback. In terms of nutrient regulation, carnitine metabolism regulates hypothalamic fatty acid sensing through the actions of CPT1 and has an underappreciated role in glucose sensing since carnitine metabolism also buffers mitochondrial matrix levels of acetyl-CoA, an allosteric inhibitor of pyruvate dehydrogenase and hence glucose metabolism. Studies also show that hypothalamic CPT1 activity also controls hormonal feedback. We hypothesis that hypothalamic carnitine metabolism represents a key molecular target that can concurrently integrate nutrient and hormonal information, which is critical to maintain energy homeostasis. We also suggest this is relevant to broader neuroendocrine research as it predicts that hormonal signaling in the brain varies depending on current nutrient status. Indeed, the metabolic action of ghrelin, leptin or insulin at POMC or NPY neurons may depend on appropriate nutrient-sensing in these neurons and we hypothesize carnitine metabolism is critical in the integrative processing. Future research is required to examine the neuron-specific effects of carnitine metabolism on concurrent nutrient- and hormonal-sensing in AgRP and POMC neurons. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Identification of potent, nonabsorbable agonists of the calcium-sensing receptor for GI-specific administration.

PubMed

Sparks, Steven M; Spearing, Paul K; Diaz, Caroline J; Cowan, David J; Jayawickreme, Channa; Chen, Grace; Rimele, Thomas J; Generaux, Claudia; Harston, Lindsey T; Roller, Shane G

2017-10-15

Modulation of gastrointestinal nutrient sensing pathways provides a promising a new approach for the treatment of metabolic diseases including diabetes and obesity. The calcium-sensing receptor has been identified as a key receptor involved in mineral and amino acid nutrient sensing and thus is an attractive target for modulation in the intestine. Herein we describe the optimization of gastrointestinally restricted calcium-sensing receptor agonists starting from a 3-aminopyrrolidine-containing template leading to the identification of GI-restricted agonist 19 (GSK3004774). Copyright © 2017 Elsevier Ltd. All rights reserved.

Investment in secreted enzymes during nutrient-limited growth is utility dependent.

PubMed

Cezairliyan, Brent; Ausubel, Frederick M

2017-09-12

Pathogenic bacteria secrete toxins and degradative enzymes that facilitate their growth by liberating nutrients from the environment. To understand bacterial growth under nutrient-limited conditions, we studied resource allocation between cellular and secreted components by the pathogenic bacterium Pseudomonas aeruginosa during growth on a protein substrate that requires extracellular digestion by secreted proteases. We identified a quantitative relationship between the rate of increase of cellular biomass under nutrient-limiting growth conditions and the rate of increase in investment in secreted proteases. Production of secreted proteases is stimulated by secreted signals that convey information about the utility of secreted proteins during nutrient-limited growth. Growth modeling using this relationship recapitulated the observed kinetics of bacterial growth on a protein substrate. The proposed regulatory strategy suggests a rationale for quorum-sensing-dependent stimulation of the production of secreted enzymes whereby investment in secreted enzymes occurs in proportion to the utility they confer. Our model provides a framework that can be applied toward understanding bacterial growth in many environments where growth rate is limited by the availability of nutrients.

Analyzing Variability in Landscape Nutrient Loading Using Spatially-Explicit Maps in the Great Lakes Basin

NASA Astrophysics Data System (ADS)

Hamlin, Q. F.; Kendall, A. D.; Martin, S. L.; Whitenack, H. D.; Roush, J. A.; Hannah, B. A.; Hyndman, D. W.

2017-12-01

Excessive loading of nitrogen and phosphorous to the landscape has caused biologically and economically damaging eutrophication and harmful algal blooms in the Great Lakes Basin (GLB) and across the world. We mapped source-specific loads of nitrogen and phosphorous to the landscape using broadly available data across the GLB. SENSMap (Spatially Explicit Nutrient Source Map) is a 30m resolution snapshot of nutrient loads ca. 2010. We use these maps to study variable nutrient loading and provide this information to watershed managers through NOAA's GLB Tipping Points Planner. SENSMap individually maps nutrient point sources and six non-point sources: 1) atmospheric deposition, 2) septic tanks, 3) non-agricultural chemical fertilizer, 4) agricultural chemical fertilizer, 5) manure, and 6) nitrogen fixation from legumes. To model source-specific loads at high resolution, SENSMap synthesizes a wide range of remotely sensed, surveyed, and tabular data. Using these spatially explicit nutrient loading maps, we can better calibrate local land use-based water quality models and provide insight to watershed managers on how to focus nutrient reduction strategies. Here we examine differences in dominant nutrient sources across the GLB, and how those sources vary by land use. SENSMap's high resolution, source-specific approach offers a different lens to understand nutrient loading than traditional semi-distributed or land use based models.

Using Hyperspectral Aircraft Remote Sensing to Support Ecosystems Services Research in New England Lakes and Ponds

NASA Astrophysics Data System (ADS)

Keith, D. J.; Milstead, B.; Walker, H.; Worthy, D.; Szykman, J.; Wusk, M.; Kagey, L.; Howell, C.; Snook, H.; Drueke, C.

2010-12-01

Northeastern lakes and ponds provide important ecosystem services to New England residents and visitors. These include the provisioning of abundant, clean water for consumption, agriculture, and industry as well as cultural services (recreation, aesthetics, and wilderness experiences) which enhance local economies and quality of life. Less understood, but equally important, are the roles that these lakes play in protecting all life through supportive services such as nutrient cycling. Nitrogen and phosphorus have a direct impact on the condition of fresh water lakes. Excesses of these nutrients can lead to eutrophication, toxic cyanobacteria blooms, decreased biodiversity, and loss of ecosystem function leading to a reduction in the availability and delivery of ecosystem services. In this study, we examined how variations in lake nutrient concentrations and phytoplankton pigment concentrations correlated with changes in the potential to provide cultural ecosystem services. Using a NASA Cessna 206 aircraft, hyperspectral data were collected during late summer 2009 from 55 lakes in New Hampshire, Massachusetts, Connecticut, and Rhode Island over a 2 day period. From the spectral data, algorithms were created which estimated concentrations of chlorophyll a, phycocyanin, and colored dissolved organic matter. The remotely sensed estimates were supplemented by in situ chlorophyll a, total nitrogen, total phosphorus and lake color data from 43 lakes sampled by field crews from the New England states. The purpose of this research is to understand how variations in lake nutrient concentrations and phytoplankton pigment concentrations correlate with changes in availability of cultural ecosystem services in the surveyed lakes. This dataset will be combined with information from the EPA National Lake Survey (2007), the EPA New England Lakes and Ponds Survey (2008) and the USGS SPARROW model to explore the association between lake condition and the provisioning of ecosystem services on a regional scale. Under the EPA Ecological Services Research Program (ESRP), this information will provide managers and researchers with a better understanding of links between management decisions affecting nutrient fluxes and impacts on selected ecosystem services.

[Defining of wheat growth management zones based on remote sensing and geostatistics].

PubMed

Huang, Yan; Zhu, Yan; Ma, Meng-Li; Wang, Hang; Cao, Wei-Xing; Tian, Yong-Chao

2011-02-01

Taking the winter wheat planting areas in Rugao City and Haian County of Jiangsu Province as test objects, the clustering defining of wheat growth management zones was made, based on the spatial variability analysis and principal component extraction of the normalized difference vegetation index (NDVI) data calculated from the HJ-1A/B CCD images (30 m resolution) at different growth stages of winter wheat, and of the soil nutrient indices (total nitrogen, organic matter, available phosphorus, and available potassium). The results showed that the integration of the NDVI at heading stage with above-mentioned soil nutrient indices produced the best results of wheat growth management zone defining, with the variation coefficients of NDVI and soil nutrient indices in each defined zone ranged in 4.5% -6.1% and 3.3% -87.9%, respectively. However, the variation coefficients were much larger when the wheat growth management zones were defined individually by NDVI or by soil nutrient indices, suggesting that the newly developed defining method could reduce the variability within the defined management zones and improve the crop management precision, and thereby, contribute to the winter wheat growth management and process simulation at regional scale.

Central nervous system regulation of intestinal lipid and lipoprotein metabolism.

PubMed

Farr, Sarah; Taher, Jennifer; Adeli, Khosrow

2016-02-01

In response to nutrient availability, the small intestine and brain closely communicate to modulate energy homeostasis and metabolism. The gut-brain axis involves complex nutrient sensing mechanisms and an integration of neuronal and hormonal signaling. This review summarizes recent evidence implicating the gut-brain axis in regulating lipoprotein metabolism, with potential implications for the dyslipidemia of insulin resistant states. The intestine and brain possess distinct mechanisms for sensing lipid availability, which triggers subsequent regulation of feeding, glucose homeostasis, and adipose tissue metabolism. More recently, central receptors, neuropeptides, and gut hormones that communicate with the brain have been shown to modulate hepatic and intestinal lipoprotein metabolism via parasympathetic and sympathetic signaling. Gut-derived glucagon-like peptides appear to be particularly important in modulating the intestinal secretion of chylomicron particles via a novel brain-gut axis. Dysregulation of these pathways may contribute to postprandial diabetic dyslipidemia. Emerging evidence implicates the central and enteric nervous systems in controlling many aspects of lipid and lipoprotein metabolism. Bidirectional communication between the gut and brain involving neuronal pathways and gut peptides is critical for regulating feeding and metabolism, and forms a neuroendocrine circuit to modulate dietary fat absorption and intestinal production of atherogenic chylomicron particles.

Inferring nutrient loading of estuarine systems by remote sensing of aquatic vegetation

NASA Technical Reports Server (NTRS)

Anderson, R. R.

1978-01-01

THe use of remote sensing to record algal and vascular aquatic plant growths in estuarine waters is discussed. A technique is proposed that uses a combination of data to hierarchically classify watersheds with regard to severity of potential pollution. Specific nonpoint sources of nutrients in tributaries of the watershed are identified with lower altitude photography of vegetation and selected ground sampling. It is concluded that excessive growths of some aquatic plants may be related to nutrient pollution.

Integration of Metabolic and Quorum Sensing Signals Governing the Decision to Cooperate in a Bacterial Social Trait

PubMed Central

Boyle, Kerry E.; Monaco, Hilary; van Ditmarsch, Dave; Deforet, Maxime; Xavier, Joao B.

2015-01-01

Many unicellular organisms live in multicellular communities that rely on cooperation between cells. However, cooperative traits are vulnerable to exploitation by non-cooperators (cheaters). We expand our understanding of the molecular mechanisms that allow multicellular systems to remain robust in the face of cheating by dissecting the dynamic regulation of cooperative rhamnolipids required for swarming in Pseudomonas aeruginosa. We combine mathematical modeling and experiments to quantitatively characterize the integration of metabolic and population density signals (quorum sensing) governing expression of the rhamnolipid synthesis operon rhlAB. The combined computational/experimental analysis reveals that when nutrients are abundant, rhlAB promoter activity increases gradually in a density dependent way. When growth slows down due to nutrient limitation, rhlAB promoter activity can stop abruptly, decrease gradually or even increase depending on whether the growth-limiting nutrient is the carbon source, nitrogen source or iron. Starvation by specific nutrients drives growth on intracellular nutrient pools as well as the qualitative rhlAB promoter response, which itself is modulated by quorum sensing. Our quantitative analysis suggests a supply-driven activation that integrates metabolic prudence with quorum sensing in a non-digital manner and allows P. aeruginosa cells to invest in cooperation only when the population size is large enough (quorum sensing) and individual cells have enough metabolic resources to do so (metabolic prudence). Thus, the quantitative description of rhlAB regulatory dynamics brings a greater understating to the regulation required to make swarming cooperation stable. PMID:26102206

Needs Assessment for the Use of NASA Remote Sensing Data in the Development and Implementation of Estuarine and Coastal Water Quality Standards

NASA Technical Reports Server (NTRS)

Spiering, Bruce; Underwood, Lauren; Ellis, Chris; Lehrter, John; Hagy, Jim; Schaeffer, Blake

2010-01-01

The goals of the project are to provide information from satellite remote sensing to support numeric nutrient criteria development and to determine data processing methods and data quality requirements to support nutrient criteria development and implementation. The approach is to identify water quality indicators that are used by decision makers to assess water quality and that are related to optical properties of the water; to develop remotely sensed data products based on algorithms relating remote sensing imagery to field-based observations of indicator values; to develop methods to assess estuarine water quality, including trends, spatial and temporal variability, and seasonality; and to develop tools to assist in the development and implementation of estuarine and coastal nutrient criteria. Additional slides present process, criteria development, typical data sources and analyses for criteria process, the power of remote sensing data for the process, examples from Pensacola Bay, spatial and temporal variability, pixel matchups, remote sensing validation, remote sensing in coastal waters, requirements for remotely sensed data products, and needs assessment. An additional presentation examines group engagement and information collection. Topics include needs assessment purpose and objectives, understanding water quality decision making, determining information requirements, and next steps.

Hyperspectral remote sensing analysis of short rotation woody crops grown with controlled nutrient and irrigation treatments

Treesearch

Jungho Im; John R. Jensen; Mark Coleman; Eric Nelson

2009-01-01

Hyperspectral remote sensing research was conducted to document the biophysical and biochemical characteristics of controlled forest plots subjected to various nutrient and irrigation treatments. The experimental plots were located on the Savannah River Site near Aiken, SC. AISA hyperspectral imagery were analysed using three approaches, including: (1) normalized...

A new parameterization for surface ocean light attenuation in Earth System Models: assessing the impact of light absorption by colored detrital material

NASA Astrophysics Data System (ADS)

Kim, G. E.; Pradal, M.-A.; Gnanadesikan, A.

2015-03-01

Light limitation can affect the distribution of biota and nutrients in the ocean. Light absorption by colored detrital material (CDM) was included in a fully coupled Earth System Model using a new parameterization for shortwave attenuation. Two model runs were conducted, with and without light attenuation by CDM. In a global average sense, greater light limitation associated with CDM increased surface chlorophyll, biomass and nutrients together. These changes can be attributed to the movement of biological productivity higher up the water column, which increased surface chlorophyll and biomass while simultaneously decreasing total biomass. Meanwhile, the reduction in biomass resulted in greater nutrient availability throughout the water column. Similar results were found on a regional scale in an analysis of the oceans by biome. In coastal regions, surface chlorophyll increased by 35% while total integrated phytoplankton biomass diminished by 18%. The largest relative increases in modeled surface chlorophyll and biomass in the open ocean were found in the equatorial biomes, while largest decreases in depth-integrated biomass and chlorophyll were found in the subpolar and polar biomes. This mismatch of surface and subsurface trends and their regional dependence was analyzed by comparing the competing factors of diminished light availability and increased nutrient availability on phytoplankton growth in the upper 200 m. Overall, increases in surface biomass were expected to accompany greater nutrient uptake and therefore diminish surface nutrients, but changes in light limitation decoupled trends between these two variables. Understanding changes in biological productivity requires both surface and depth-resolved information. Surface trends may be minimal or of the opposite sign to depth-integrated amounts, depending on the vertical structure of phytoplankton abundance.

Role of insulin, adipocyte hormones, and nutrient-sensing pathways in regulating fuel metabolism and energy homeostasis: a nutritional perspective of diabetes, obesity, and cancer.

PubMed

Marshall, Stephen

2006-08-01

Traditionally, nutrients such as glucose and amino acids have been viewed as substrates for the generation of high-energy molecules and as precursors for the biosynthesis of macromolecules. However, it is now apparent that nutrients also function as signaling molecules in functionally diverse signal transduction pathways. Glucose and amino acids trigger signaling cascades that regulate various aspects of fuel and energy metabolism and control the growth, proliferation, and survival of cells. Here, we provide a functional and regulatory overview of three well-established nutrient signaling pathways-the hexosamine signaling pathway, the mTOR (mammalian target of rapamycin) signaling pathway, and the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway. Nutrient signaling pathways are interconnected, coupled to insulin signaling, and linked to the release of metabolic hormones from adipose tissue. Thus, nutrient signaling pathways do not function in isolation. Rather, they appear to serve as components of a larger "metabolic regulatory network" that controls fuel and energy metabolism (at the cell, tissue, and whole-body levels) and links nutrient availability with cell growth and proliferation. Understanding the diverse roles of nutrients and delineating nutrient signaling pathways should facilitate drug discovery research and the search for novel therapeutic compounds to prevent and treat various human diseases such as diabetes, obesity, and cancer.

Involvement of Small RNAs in Phosphorus and Sulfur Sensing, Signaling and Stress: Current Update

PubMed Central

Kumar, Smita; Verma, Saurabh; Trivedi, Prabodh K.

2017-01-01

Plants require several essential mineral nutrients for their growth and development. These nutrients are required to maintain physiological processes and structural integrity in plants. The root architecture has evolved to absorb nutrients from soil and transport them to other parts of the plant. Nutrient deficiency affects several physiological and biological processes in plants and leads to reduction in crop productivity and yield. To compensate this adversity, plants have developed adaptive mechanisms to enhance the acquisition, conservation, and mobilization of these nutrients under deficient or adverse conditions. In addition, plants have evolved an intricate nexus of complex signaling cascades, which help in nutrient sensing and uptake as well as to maintain nutrient homeostasis. In recent years, small non-coding RNAs such as micro RNAs (miRNAs) and endogenous small interfering RNAs have emerged as important component in regulating plant stress responses. A set of these small RNAs (sRNAs) have been implicated in regulating various processes involved in nutrient uptake, assimilation, and deficiency. In response to phosphorus (P) and sulphur (S) deficiencies, role of sRNAs, miR395 and miR399, have been identified to be instrumental; however, many more miRNAs might be involved in regulating the plant response to these nutrient stresses. These sRNAs modulate expression of target genes in response to P and S deficiencies and regulate their uptake and utilization for proper growth and development of the plant. This review summarizes the current understanding of uptake, sensing, and signaling of P and S and highlights the regulatory role of sRNAs in adaptive responses to these nutrient stresses in plants. PMID:28344582

Multifunctional Nutrient-Binding Proteins Adapt Human Symbiotic Bacteria for Glycan Competition in the Gut by Separately Promoting Enhanced Sensing and Catalysis

PubMed Central

Cameron, Elizabeth A.; Kwiatkowski, Kurt J.; Lee, Byung-Hoo; Hamaker, Bruce R.; Koropatkin, Nicole M.

2014-01-01

ABSTRACT To compete for the dynamic stream of nutrients flowing into their ecosystem, colonic bacteria must respond rapidly to new resources and then catabolize them efficiently once they are detected. The Bacteroides thetaiotaomicron starch utilization system (Sus) is a model for nutrient acquisition by symbiotic gut bacteria, which harbor thousands of related Sus-like systems. Structural investigation of the four Sus outer membrane proteins (SusD, -E, -F, and -G) revealed that they contain a total of eight starch-binding sites that we demonstrated, using genetic and biochemical approaches, to play distinct roles in starch metabolism in vitro and in vivo in gnotobiotic mice. SusD, whose homologs are abundant in the human microbiome, is critical for the initial sensing of available starch, allowing sus transcriptional activation at much lower concentrations than without this function. In contrast, seven additional binding sites across SusE, -F, and -G are dispensable for sus activation. However, they optimize the rate of growth on starch in a manner dependent on the expression of the bacterial polysaccharide capsule, suggesting that they have evolved to offset the diffusion barrier created by this structure. These findings demonstrate how proteins with similar biochemical behavior can serve orthogonal functions during different stages of cellular adaptation to nutrients. Finally, we demonstrated in gnotobiotic mice fed a starch-rich diet that the Sus binding sites confer a competitive advantage to B. thetaiotaomicron in vivo in a manner that is dependent on other colonizing microbes. This study reveals how numerically dominant families of carbohydrate-binding proteins in the human microbiome fulfill separate and sometimes cooperative roles to optimize gut commensal bacteria for nutrient acquisition. PMID:25205092

mTOR-dependent activation of the transcription factor TIF-IA links rRNA synthesis to nutrient availability.

PubMed

Mayer, Christine; Zhao, Jian; Yuan, Xuejun; Grummt, Ingrid

2004-02-15

In cycling cells, transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with cell growth. Here, we show that the mammalian target of rapamycin (mTOR) regulates Pol I transcription by modulating the activity of TIF-IA, a regulatory factor that senses nutrient and growth-factor availability. Inhibition of mTOR signaling by rapamycin inactivates TIF-IA and impairs transcription-initiation complex formation. Moreover, rapamycin treatment leads to translocation of TIF-IA into the cytoplasm. Rapamycin-mediated inactivation of TIF-IA is caused by hypophosphorylation of Se 44 (S44) and hyperphosphorylation of Se 199 (S199). Phosphorylation at these sites affects TIF-IA activity in opposite ways, for example, phosphorylation of S44 activates and S199 inactivates TIF-IA. The results identify a new target formTOR-signaling pathways and elucidate the molecular mechanism underlying mTOR-dependent regulation of RNA synthesis.

mTORC1 in AGRP neurons integrates exteroceptive and interoceptive food-related cues in the modulation of adaptive energy expenditure in mice

PubMed Central

Burke, Luke K; Darwish, Tamana; Cavanaugh, Althea R; Virtue, Sam; Roth, Emma; Morro, Joanna; Liu, Shun-Mei; Xia, Jing; Dalley, Jeffrey W; Burling, Keith; Chua, Streamson; Vidal-Puig, Toni; Schwartz, Gary J; Blouet, Clémence

2017-01-01

Energy dissipation through interscapular brown adipose tissue (iBAT) thermogenesis is an important contributor to adaptive energy expenditure. However, it remains unresolved how acute and chronic changes in energy availability are detected by the brain to adjust iBAT activity and maintain energy homeostasis. Here, we provide evidence that AGRP inhibitory tone to iBAT represents an energy-sparing circuit that integrates environmental food cues and internal signals of energy availability. We establish a role for the nutrient-sensing mTORC1 signaling pathway within AGRP neurons in the detection of environmental food cues and internal signals of energy availability, and in the bi-directional control of iBAT thermogenesis during nutrient deficiency and excess. Collectively, our findings provide insights into how mTORC1 signaling within AGRP neurons surveys energy availability to engage iBAT thermogenesis, and identify AGRP neurons as a neuronal substrate for the coordination of energy intake and adaptive expenditure under varying physiological and environmental contexts. DOI: http://dx.doi.org/10.7554/eLife.22848.001 PMID:28532548

NutrimiRAging: Micromanaging Nutrient Sensing Pathways through Nutrition to Promote Healthy Aging.

PubMed

Micó, Víctor; Berninches, Laura; Tapia, Javier; Daimiel, Lidia

2017-04-26

Current sociodemographic predictions point to a demographic shift in developed and developing countries that will result in an unprecedented increase of the elderly population. This will be accompanied by an increase in age-related conditions that will strongly impair human health and quality of life. For this reason, aging is a major concern worldwide. Healthy aging depends on a combination of individual genetic factors and external environmental factors. Diet has been proved to be a powerful tool to modulate aging and caloric restriction has emerged as a valuable intervention in this regard. However, many questions about how a controlled caloric restriction intervention affects aging-related processes are still unanswered. Nutrient sensing pathways become deregulated with age and lose effectiveness with age. These pathways are a link between diet and aging. Thus, fully understanding this link is a mandatory step before bringing caloric restriction into practice. MicroRNAs have emerged as important regulators of cellular functions and can be modified by diet. Some microRNAs target genes encoding proteins and enzymes belonging to the nutrient sensing pathways and, therefore, may play key roles in the modulation of the aging process. In this review, we aimed to show the relationship between diet, nutrient sensing pathways and microRNAs in the context of aging.

Interplay between Oxidative Stress and Nutrient Sensing Signaling in the Developmental Origins of Cardiovascular Disease

PubMed Central

Tain, You-Lin; Hsu, Chien-Ning

2017-01-01

Cardiovascular disease (CVD) presents a global health burden, despite recent advances in management. CVD can originate from early life by so-called “developmental origins of health and disease” (DOHaD). Epidemiological and experimental evidence supports that early-life insults can induce programming of later CVD. Underlying the DOHaD concept, early intervention may offset programming process to prevent the development of CVD, namely reprogramming. Oxidative stress and nutrient sensing signals have been considered to be major mechanisms of cardiovascular programming, while the interplay between these two mechanisms have not been examined in detail. This review summarizes current evidence that supports the link between oxidative stress and nutrient sensing signaling to cardiovascular programming, with an emphasis on the l-arginine–asymmetric dimethylarginine (ADMA)–nitric oxide (NO) pathway. This review provides an overview of evidence from human studies supporting fetal programming of CVD, insight from animal models of cardiovascular programming and oxidative stress, impact of the l-arginine–ADMA–NO pathway in cardiovascular programming, the crosstalk between l-arginine metabolism and nutrient sensing signals, and application of reprogramming interventions to prevent the programming of CVD. A greater understanding of the mechanisms underlying cardiovascular programming is essential to developing early reprogramming interventions to combat the globally growing epidemic of CVD. PMID:28420139

Diet and Energy-Sensing Inputs Affect TorC1-Mediated Axon Misrouting but Not TorC2-Directed Synapse Growth in a Drosophila Model of Tuberous Sclerosis

PubMed Central

Dimitroff, Brian; Lee, Hyun-Gwan; Zhao, Na; O'Connor, Michael B.; Neufeld, Thomas P.; Selleck, Scott B.

2012-01-01

The Target of Rapamycin (TOR) growth regulatory system is influenced by a number of different inputs, including growth factor signaling, nutrient availability, and cellular energy levels. While the effects of TOR on cell and organismal growth have been well characterized, this pathway also has profound effects on neural development and behavior. Hyperactivation of the TOR pathway by mutations in the upstream TOR inhibitors TSC1 (tuberous sclerosis complex 1) or TSC2 promotes benign tumors and neurological and behavioral deficits, a syndrome known as tuberous sclerosis (TS). In Drosophila, neuron-specific overexpression of Rheb, the direct downstream target inhibited by Tsc1/Tsc2, produced significant synapse overgrowth, axon misrouting, and phototaxis deficits. To understand how misregulation of Tor signaling affects neural and behavioral development, we examined the influence of growth factor, nutrient, and energy sensing inputs on these neurodevelopmental phenotypes. Neural expression of Pi3K, a principal mediator of growth factor inputs to Tor, caused synapse overgrowth similar to Rheb, but did not disrupt axon guidance or phototaxis. Dietary restriction rescued Rheb-mediated behavioral and axon guidance deficits, as did overexpression of AMPK, a component of the cellular energy sensing pathway, but neither was able to rescue synapse overgrowth. While axon guidance and behavioral phenotypes were affected by altering the function of a Tor complex 1 (TorC1) component, Raptor, or a TORC1 downstream element (S6k), synapse overgrowth was only suppressed by reducing the function of Tor complex 2 (TorC2) components (Rictor, Sin1). These findings demonstrate that different inputs to Tor signaling have distinct activities in nervous system development, and that Tor provides an important connection between nutrient-energy sensing systems and patterning of the nervous system. PMID:22319582

Automated lettuce nutrient solution management using an array of ion-selective electrodes

USDA-ARS?s Scientific Manuscript database

Automated sensing and control of macronutrients in hydroponic solutions would allow more efficient management of nutrients for crop growth in closed systems. This paper describes the development and evaluation of a computer-controlled nutrient management system with an array of ion-selective electro...

Substrate quality and nutrient availability influence CO2 production from tropical peat decomposition

NASA Astrophysics Data System (ADS)

Swails, E.; Jaye, D.; Verchot, L. V.; Hergoualc'h, K.; Wahyuni, N. S.; Borchard, N.; Lawrence, D.

2015-12-01

In Indonesia, peatlands are a major and growing source of greenhouse gas emissions due to increasing pressure from oil palm and pulp wood plantations. We are using a combination of field measures, laboratory experiments, and remote sensing to investigate relationships among land use, climatic factors and biogeochemical controls, and their influence on trace gas fluxes from tropical peat soils. Analysis of soils collected from peat sites on two major islands indicated substantial variation in peat substrate quality and nutrient content among land uses and geographic location. We conducted laboratory incubations to test the influence of substrate quality and nutrient availability on CO2 production from peat decomposition. Differences in peat characteristics attributable to land use change were tested by comparison of forest and oil palm peat samples collected from the same peat dome in Kalimantan. Regional differences in peat characteristics were tested by comparison of samples from Sumatra with samples from Kalimantan. We conducted additional experiments to test the influence of N and P availability and labile carbon on CO2 production. Under moisture conditions typical of oil palm plantations, CO2 production was higher from peat forest samples than from oil palm samples. CO2 production from Sumatra and Kalimantan oil palm samples was not different, despite apparent differences in nutrient content of these soils. N and P treatments representative of fertilizer application rates raised CO2 production from forest samples but not oil palm samples. Labile carbon treatments raised CO2 production in all samples. Our results suggest that decomposition of peat forest soils is nutrient limited, while substrate quality controls decomposition of oil palm soils post-conversion. Though fertilizer application could accelerate peat decomposition initially, fertilizer application may not influence long-term CO2 emissions from oil palm on peat.

Sensing the fuels: glucose and lipid signaling in the CNS controlling energy homeostasis.

PubMed

Jordan, Sabine D; Könner, A Christine; Brüning, Jens C

2010-10-01

The central nervous system (CNS) is capable of gathering information on the body's nutritional state and it implements appropriate behavioral and metabolic responses to changes in fuel availability. This feedback signaling of peripheral tissues ensures the maintenance of energy homeostasis. The hypothalamus is a primary site of convergence and integration for these nutrient-related feedback signals, which include central and peripheral neuronal inputs as well as hormonal signals. Increasing evidence indicates that glucose and lipids are detected by specialized fuel-sensing neurons that are integrated in these hypothalamic neuronal circuits. The purpose of this review is to outline the current understanding of fuel-sensing mechanisms in the hypothalamus, to integrate the recent findings in this field, and to address the potential role of dysregulation in these pathways in the development of obesity and type 2 diabetes mellitus.

Nutrient sensing signaling integrates nutrient metabolism and intestinal immunity in grass carp, Ctenopharyngodon idellus after prolonged starvation.

PubMed

Li, Shan; Li, Jiabo; Zhao, Yongliang; Zhang, Qin; Wang, Qingchao

2017-12-01

Starvation has been shown to affect growth and nutrient metabolism in fish; however, little information about the nutrient sensing signaling and mucosal adaptive immunity in fish was known. In the present study, grass carp was starved for 8weeks to simulate the natural aquaculture practice in Hubei during winter. The histology of liver was significantly affected with decreased expression of tight junction proteins including claudin-3, claudin-b and ZO-1. Muscle gene expression was also affected, with decreased expression of muscle growth promoting factors such as Myogenin, MyoD, Myf5, and increased expression of muscle degradation factors, such as CathepsinD. In addition, mucosal adaptive immunity was also significantly affected, with decreased expression of antibodies including IgZ and IgM in gut. Along with these changes was the inhibition of several nutrient sensing signaling including MAPK and TOR signaling, which leads to the inhibition of the synthesis of protein including immunoglobulin. The increased phosphorylation of eIF2α not only inhibited the translation, but also resulted in the decreased expression of IkB and increased expression of NF-B, with the activation of pro-inflammatory genes including IL8 and TNF. Copyright © 2017 Elsevier Ltd. All rights reserved.

Determination of phytoplankton chlorophyll concentrations in the Chesapeake Bay with aircraft remote sensing

NASA Technical Reports Server (NTRS)

Harding, Lawrence W., Jr.; Itsweire, Eric C.; Esaias, Wayne E.

1992-01-01

Remote sensing measurements of the distribution of phytoplankton chlorophyll concentrations in Chesapeake Bay during 1989 are described. It is shown that remote sensing from light aircraft can complement and extend measurements made from traditional platforms and provide data of improved temporal and spatial resolution, leading to a better understanding of phytoplankton dynamics in the estuary. The developments of the winter-spring diatom bloom in the polyhaline to mesohaline regions of the estuary and of the late-spring and summer dinoflagellate blooms in oligohaline and mesohaline regions are traced. The study presents the local chlorophyll algorithm developed using the NASA Ocean Data Acquisition System data and in situ chlorophyll data, interpolated maps of chlorophyll concentration generated by applying the algorithm to aircraft radiance data, ancillary in situ data on nutrients, turbidity, streamflow, and light availability, and an interpretation of phytoplankton dynamics in terms of the chlorophyll distribution in Chesapeake Bay during 1989.

Examining Dehydration and Hypoxic Stress in Wheat Plants Using a Porous Tube Plant Nutrient Delivery System Developed for Microgravity

NASA Technical Reports Server (NTRS)

Dreschel, T. W.; Hall, C. R.; Foster, T. E.; Salganic, M.; Warren, L.; Corbett, M.

2005-01-01

The Porous Tube Plant Nutrient Delivery System (PTPNDS) was designed for NASA to grow plants in microgravity of space. The system utilizes a controlled fluid loop to supply nutrients and water to plant roots growing on a ceramic surface moistened by capiflary action. A PTPNDS test bed was developed and utilizing remote sensing systems, spectral analyses procedures, gas-exchange, and fluorescence measurements, we examined differences in plant water status for wheat plants (Triticum aestivum, cv. Perigee) grown in a modified growth chamber during the summers of 2003 and 2004. Some differences in plant performance were detectable in the gas-exchange and fluorescence measurements. For instance, in both years the plants grown with the most available water had the lowest rates of photosynthesis and exhibited higher proportions of non-photochemical quenching particularly under low light levels. In addition, small differences in mean leaf water content between treatments were detected using spectral reflectance analyses.

Emergence of robust growth laws from optimal regulation of ribosome synthesis.

PubMed

Scott, Matthew; Klumpp, Stefan; Mateescu, Eduard M; Hwa, Terence

2014-08-22

Bacteria must constantly adapt their growth to changes in nutrient availability; yet despite large-scale changes in protein expression associated with sensing, adaptation, and processing different environmental nutrients, simple growth laws connect the ribosome abundance and the growth rate. Here, we investigate the origin of these growth laws by analyzing the features of ribosomal regulation that coordinate proteome-wide expression changes with cell growth in a variety of nutrient conditions in the model organism Escherichia coli. We identify supply-driven feedforward activation of ribosomal protein synthesis as the key regulatory motif maximizing amino acid flux, and autonomously guiding a cell to achieve optimal growth in different environments. The growth laws emerge naturally from the robust regulatory strategy underlying growth rate control, irrespective of the details of the molecular implementation. The study highlights the interplay between phenomenological modeling and molecular mechanisms in uncovering fundamental operating constraints, with implications for endogenous and synthetic design of microorganisms. © 2014 The Authors. Published under the terms of the CC BY 4.0 license.

Sensing the Environment Through Sestrins: Implications for Cellular Metabolism.

PubMed

Parmigiani, A; Budanov, A V

2016-01-01

Sestrins are a family of stress-responsive genes that have evolved to attenuate damage induced by stress caused to the cell. By virtue of their antioxidant activity, protein products of Sestrin genes prevent the accumulation of reactive oxygen species within the cell, thereby attenuating the detrimental effects of oxidative stress. In parallel, Sestrins participate in several signaling pathways that control the activity of the target of rapamycin protein kinase (TOR). TOR is a crucial sensor of intracellular and extracellular conditions that promotes cell growth and anabolism when nutrients and growth factors are abundant. In addition to reacting to stress-inducing insults, Sestrins also monitor the changes in the availability of nutrients, which allows them to serve as a key checkpoint for the TOR-regulated signaling pathways. In this review, we will discuss how Sestrins integrate signals from numerous stress- and nutrient-responsive signaling pathways to orchestrate cellular metabolism and support cell viability. Copyright © 2016 Elsevier Inc. All rights reserved.

Human CLEC16A regulates autophagy through modulating mTOR activity.

PubMed

Tam, Rachel Chun Yee; Li, Michelle Wing Man; Gao, Yan Pan; Pang, Yuen Ting; Yan, Sheng; Ge, Wei; Lau, Chak Sing; Chan, Vera Sau Fong

2017-03-15

CLEC16A is genetically linked with multiple autoimmune disorders but its functional relevance in autoimmunity remains obscure. Recent evidence has signposted the emerging role of autophagy in autoimmune disease development. Here, by ectopic expression and siRNA silencing, we show that CLEC16A has an inhibitory role in starvation-induced autophagy in human cells. Combining quantitative proteomics and immunoblotting analyses, we found that CLEC16A likely regulates autophagy by activating mTOR pathway. Overexpression of CLEC16A was found to sensitize cells towards the availability of nutrients, resulting in a heightened mTOR activity, which in turn diminished LC3 autophagic activity following nutrient deprivation. CLEC16A deficiency, on the other hand, delayed mTOR activity in response to nutrient sensing, thereby resulted in an augmented autophagic response. CLEC16A was found residing in cytosolic vesicles and the Golgi, and nutrient removal promoted a stronger clustering within the Golgi, where it was possibly in a vantage position to activate mTOR upon nutrient replenishment. These findings suggest that Golgi-associated CLEC16A negatively regulates autophagy via modulation of mTOR activity, and may provide support for a functional link between CLEC16A and autoimmunity. Copyright © 2017 Elsevier Inc. All rights reserved.

mTOR-dependent activation of the transcription factor TIF-IA links rRNA synthesis to nutrient availability

PubMed Central

Mayer, Christine; Zhao, Jian; Yuan, Xuejun; Grummt, Ingrid

2004-01-01

In cycling cells, transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with cell growth. Here, we show that the mammalian target of rapamycin (mTOR) regulates Pol I transcription by modulating the activity of TIF-IA, a regulatory factor that senses nutrient and growth-factor availability. Inhibition of mTOR signaling by rapamycin inactivates TIF-IA and impairs transcription-initiation complex formation. Moreover, rapamycin treatment leads to translocation of TIF-IA into the cytoplasm. Rapamycin-mediated inactivation of TIF-IA is caused by hypophosphorylation of Ser 44 (S44) and hyperphosphorylation of Ser 199 (S199). Phosphorylation at these sites affects TIF-IA activity in opposite ways, for example, phosphorylation of S44 activates and S199 inactivates TIF-IA. The results identify a new target for mTOR-signaling pathways and elucidate the molecular mechanism underlying mTOR-dependent regulation of rRNA synthesis. PMID:15004009

The production of aromatic alcohols in non-Saccharomyces wine yeast is modulated by nutrient availability.

PubMed

González, Beatriz; Vázquez, Jennifer; Morcillo-Parra, M Ángeles; Mas, Albert; Torija, María Jesús; Beltran, Gemma

2018-09-01

Aromatic alcohols (tryptophol, phenylethanol, tyrosol) positively contribute to organoleptic characteristics of wines, and are also described as bioactive compounds and quorum sensing molecules. These alcohols are produced by yeast during alcoholic fermentation via the Erhlich pathway, although in non-Saccharomyces this production has been poorly studied. We studied how different wine yeast species modulate the synthesis patterns of aromatic alcohol production depending on glucose, nitrogen and aromatic amino acid availability. Nitrogen limitation strongly promoted the production of aromatic alcohols in all strains, whereas low glucose generally inhibited it. Increased aromatic amino acid concentrations stimulated the production of aromatic alcohols in all of the strains and conditions tested. Thus, there was a clear association between the nutrient conditions and production of aromatic alcohols in most of the wine yeast species analysed. Additionally, the synthesis pattern of these alcohols has been evaluated for the first time in Torulaspora delbrueckii, Metschnikowia pulcherrima and Starmellera bacillaris. Copyright © 2018 Elsevier Ltd. All rights reserved.

Terrestrial biogeochemical cycles - Global interactions with the atmosphere and hydrology

NASA Technical Reports Server (NTRS)

Schimel, David S.; Parton, William J.; Kittel, Timothy G. F.

1991-01-01

A review is presented of developments in ecosystem theory, remote sensing, and geographic information systems that support new endeavors in spatial modeling. A paradigm has emerged to predict ecosystem behavior based on understanding responses to multiple resources. Ecosystem models couple primary production to decomposition and nutrient availability utilizing this paradigm. It is indicated that coupling of transport and ecosystem processes alters the behavior of earth system components (terrestrial ecosystems, hydrology, and the atmosphere) from that of an uncoupled model.

Monitoring boreal ecosystem phenology with integrated active/passive microwave remote sensing

NASA Technical Reports Server (NTRS)

McDonald, K. C.; Njoku, E.; Kimball, J.; Running, S.; Thompson, C.; Lee, J. K.

2002-01-01

The important role of the high latitudes in the functioning of global processes is becoming well established. The size and remoteness of arctic and boreal ecosystems, however, pose a challenge to quantification of both terrestrial ecosystem processes and their feedbacks to regional and global climate conditions. Boreal and arctic regions form a complex land cover mosaic where vegetation structure, condition and distribution are strongly regulated by environmental factors such as moisture availability, permafrost, growing season length, disturbance and soil nutrients.

mTORC1 as the main gateway to autophagy

PubMed Central

Rabanal-Ruiz, Yoana; Otten, Elsje G.; Korolchuk, Viktor I.

2017-01-01

Cells and organisms must coordinate their metabolic activity with changes in their environment to ensure their growth only when conditions are favourable. In order to maintain cellular homoeostasis, a tight regulation between the synthesis and degradation of cellular components is essential. At the epicentre of the cellular nutrient sensing is the mechanistic target of rapamycin complex 1 (mTORC1) which connects environmental cues, including nutrient and growth factor availability as well as stress, to metabolic processes in order to preserve cellular homoeostasis. Under nutrient-rich conditions mTORC1 promotes cell growth by stimulating biosynthetic pathways, including synthesis of proteins, lipids and nucleotides, and by inhibiting cellular catabolism through repression of the autophagic pathway. Its close signalling interplay with the energy sensor AMP-activated protein kinase (AMPK) dictates whether the cell actively favours anabolic or catabolic processes. Underlining the role of mTORC1 in the coordination of cellular metabolism, its deregulation is linked to numerous human diseases ranging from metabolic disorders to many cancers. Although mTORC1 can be modulated by a number of different inputs, amino acids represent primordial cues that cannot be compensated for by any other stimuli. The understanding of how amino acids signal to mTORC1 has increased considerably in the last years; however this area of research remains a hot topic in biomedical sciences. The current ideas and models proposed to explain the interrelationship between amino acid sensing, mTORC1 signalling and autophagy is the subject of the present review. PMID:29233869

Nutrient Estimation Using Subsurface Sensing Methods

USDA-ARS?s Scientific Manuscript database

This report investigates the use of precision management techniques for measuring soil conductivity on feedlot surfaces to estimate nutrient value for crop production. An electromagnetic induction soil conductivity meter was used to collect apparent soil electrical conductivity (ECa) from feedlot p...

Regulation of Manganese Antioxidants by Nutrient Sensing Pathways in Saccharomyces cerevisiae

PubMed Central

Reddi, Amit R.; Culotta, Valeria C.

2011-01-01

In aerobic organisms, protection from oxidative damage involves the combined action of enzymatic and nonproteinaceous cellular factors that collectively remove harmful reactive oxygen species. One class of nonproteinaceous antioxidants includes small molecule complexes of manganese (Mn) that can scavenge superoxide anion radicals and provide a backup for superoxide dismutase enzymes. Such Mn antioxidants have been identified in diverse organisms; however, nothing regarding their physiology in the context of cellular adaptation to stress was known. Using a molecular genetic approach in Bakers’ yeast, Saccharomyces cerevisiae, we report that the Mn antioxidants can fall under control of the same pathways used for nutrient sensing and stress responses. Specifically, a serine/threonine PAS-kinase, Rim15p, that is known to integrate phosphate, nitrogen, and carbon sensing, can also control Mn antioxidant activity in yeast. Rim15p is negatively regulated by the phosphate-sensing kinase complex Pho80p/Pho85p and by the nitrogen-sensing Akt/S6 kinase homolog, Sch9p. We observed that loss of either of these upstream kinase sensors dramatically inhibited the potency of Mn as an antioxidant. Downstream of Rim15p are transcription factors Gis1p and the redundant Msn2/Msn4p pair that typically respond to nutrient and stress signals. Both transcription factors were found to modulate the potency of the Mn antioxidant but in opposing fashions: loss of Gis1p was seen to enhance Mn antioxidant activity whereas loss of Msn2/4p greatly suppressed it. Our observed roles for nutrient and stress response kinases and transcription factors in regulating the Mn antioxidant underscore its physiological importance in aerobic fitness. PMID:21926297

"Nutrient-sensing" and self-renewal: O-GlcNAc in a new role.

PubMed

Sharma, Nikita S; Saluja, Ashok K; Banerjee, Sulagna

2018-06-01

Whether embryonic, hematopoietic or cancer stem cells, this metabolic reprogramming is dependent on the nutrient-status and bioenergetic pathways that is influenced by the micro-environmental niches like hypoxia. Thus, the microenvironment plays a vital role in determining the stem cell fate by inducing metabolic reprogramming. Under the influence of the microenvironment, like hypoxia, the stem cells have increased glucose and glutamine uptake which result in activation of hexosamine biosynthesis pathway (HBP) and increased O-GlcNAc Transferase (OGT). The current review is focused on understanding how HBP, a nutrient-sensing pathway (that leads to increased OGT activity) is instrumental in regulating self-renewal not only in embryonic and hematopoietic stem cells (ESC/HSC) but also in cancer stem cells.

Mechanistic Target of Rapamycin Is a Novel Molecular Mechanism Linking Folate Availability and Cell Function.

PubMed

Silva, Elena; Rosario, Fredrick J; Powell, Theresa L; Jansson, Thomas

2017-07-01

Folate deficiency has been linked to a wide range of disorders, including cancer, neural tube defects, and fetal growth restriction. Folate regulates cellular function mediated by its involvement in the synthesis of nucleotides, which are needed for DNA synthesis, and its function as a methyl donor, which is critical for DNA methylation. Here we review current data showing that folate sensing by mechanistic target of rapamycin (mTOR) constitutes a novel and distinct pathway by which folate modulates cell functions such as nutrient transport, protein synthesis, and mitochondrial respiration. The mTOR signaling pathway responds to growth factors and changes in nutrient availability to control cell growth, proliferation, and metabolism. mTOR exists in 2 complexes, mTOR complex (mTORC) 1 and mTORC2, which have distinct upstream regulators and downstream targets. Folate deficiency in pregnant mice caused a marked inhibition of mTORC1 and mTORC2 signaling in multiple maternal and fetal tissues, downregulation of placental amino acid transporters, and fetal growth restriction. In addition, folate deficiency in primary human trophoblast (PHT) cells resulted in inhibition of mTORC1 and mTORC2 signaling and decreased the activity of key amino acid transporters. Folate sensing by mTOR in PHT cells is independent of the accumulation of homocysteine and requires the proton-coupled folate transporter (PCFT; solute carrier 46A1). Furthermore, mTORC1 and mTORC2 regulate trophoblast folate uptake by modulating the cell surface expression of folate receptor α and the reduced folate carrier. These findings, which provide a novel link between folate availability and cell function, growth, and proliferation, may have broad biological significance given the critical role of folate in normal cell function and the multiple diseases that have been associated with decreased or excessive folate availability. Low maternal folate concentrations are linked to restricted fetal growth, and we propose that the underlying mechanisms involve trophoblast mTOR folate sensing resulting in inhibition of mTORC1 and mTORC2 and downregulation of placental amino acid transporters. © 2017 American Society for Nutrition.

Coregulation of host-adapted metabolism and virulence by pathogenic yersiniae

PubMed Central

Heroven, Ann Kathrin; Dersch, Petra

2014-01-01

Deciphering the principles how pathogenic bacteria adapt their metabolism to a specific host microenvironment is critical for understanding bacterial pathogenesis. The enteric pathogenic Yersinia species Yersinia pseudotuberculosis and Yersinia enterocolitica and the causative agent of plague, Yersinia pestis, are able to survive in a large variety of environmental reservoirs (e.g., soil, plants, insects) as well as warm-blooded animals (e.g., rodents, pigs, humans) with a particular preference for lymphatic tissues. In order to manage rapidly changing environmental conditions and interbacterial competition, Yersinia senses the nutritional composition during the course of an infection by special molecular devices, integrates this information and adapts its metabolism accordingly. In addition, nutrient availability has an impact on expression of virulence genes in response to C-sources, demonstrating a tight link between the pathogenicity of yersiniae and utilization of nutrients. Recent studies revealed that global regulatory factors such as the cAMP receptor protein (Crp) and the carbon storage regulator (Csr) system are part of a large network of transcriptional and posttranscriptional control strategies adjusting metabolic changes and virulence in response to temperature, ion and nutrient availability. Gained knowledge about the specific metabolic requirements and the correlation between metabolic and virulence gene expression that enable efficient host colonization led to the identification of new potential antimicrobial targets. PMID:25368845

Taste receptors of the gut: emerging roles in health and disease.

PubMed

Depoortere, Inge

2014-01-01

Recent progress in unravelling the nutrient-sensing mechanisms in the taste buds of the tongue has triggered studies on the existence and role of chemosensory cells in the gut. Indeed, the gastrointestinal tract is the key interface between food and the human body and can sense basic tastes in much the same way as the tongue, through the use of similar G-protein-coupled taste receptors. These receptors 'taste' the luminal content and transmit signals that regulate nutrient transporter expression and nutrient uptake, and also the release of gut hormones and neurotransmitters involved in the regulation of energy and glucose homeostasis. Hence, they play a prominent role in the communication between the lumen, epithelium, smooth muscle cells, afferent nerve fibres and the brain to trigger adaptive responses that affect gastrointestinal function, food intake and glucose metabolism. This review summarises how sensing of nutrients by taste receptors along the gut plays a key role in the process of digestion, and how disturbances or adaptations of these chemosensory signalling pathways may contribute to the induction or resolution of a number of pathological conditions related to diabetes, obesity, or diet-induced symptom generation in irritable bowel syndrome. Targeting these receptors may represent a promising novel route for the treatment of a number of these diseases.

Biomimicry of quorum sensing using bacterial lifecycle model.

PubMed

Niu, Ben; Wang, Hong; Duan, Qiqi; Li, Li

2013-01-01

Recent microbiologic studies have shown that quorum sensing mechanisms, which serve as one of the fundamental requirements for bacterial survival, exist widely in bacterial intra- and inter-species cell-cell communication. Many simulation models, inspired by the social behavior of natural organisms, are presented to provide new approaches for solving realistic optimization problems. Most of these simulation models follow population-based modelling approaches, where all the individuals are updated according to the same rules. Therefore, it is difficult to maintain the diversity of the population. In this paper, we present a computational model termed LCM-QS, which simulates the bacterial quorum-sensing (QS) mechanism using an individual-based modelling approach under the framework of Agent-Environment-Rule (AER) scheme, i.e. bacterial lifecycle model (LCM). LCM-QS model can be classified into three main sub-models: chemotaxis with QS sub-model, reproduction and elimination sub-model and migration sub-model. The proposed model is used to not only imitate the bacterial evolution process at the single-cell level, but also concentrate on the study of bacterial macroscopic behaviour. Comparative experiments under four different scenarios have been conducted in an artificial 3-D environment with nutrients and noxious distribution. Detailed study on bacterial chemotatic processes with quorum sensing and without quorum sensing are compared. By using quorum sensing mechanisms, artificial bacteria working together can find the nutrient concentration (or global optimum) quickly in the artificial environment. Biomimicry of quorum sensing mechanisms using the lifecycle model allows the artificial bacteria endowed with the communication abilities, which are essential to obtain more valuable information to guide their search cooperatively towards the preferred nutrient concentrations. It can also provide an inspiration for designing new swarm intelligence optimization algorithms, which can be used for solving the real-world problems.

Biomimicry of quorum sensing using bacterial lifecycle model

PubMed Central

2013-01-01

Background Recent microbiologic studies have shown that quorum sensing mechanisms, which serve as one of the fundamental requirements for bacterial survival, exist widely in bacterial intra- and inter-species cell-cell communication. Many simulation models, inspired by the social behavior of natural organisms, are presented to provide new approaches for solving realistic optimization problems. Most of these simulation models follow population-based modelling approaches, where all the individuals are updated according to the same rules. Therefore, it is difficult to maintain the diversity of the population. Results In this paper, we present a computational model termed LCM-QS, which simulates the bacterial quorum-sensing (QS) mechanism using an individual-based modelling approach under the framework of Agent-Environment-Rule (AER) scheme, i.e. bacterial lifecycle model (LCM). LCM-QS model can be classified into three main sub-models: chemotaxis with QS sub-model, reproduction and elimination sub-model and migration sub-model. The proposed model is used to not only imitate the bacterial evolution process at the single-cell level, but also concentrate on the study of bacterial macroscopic behaviour. Comparative experiments under four different scenarios have been conducted in an artificial 3-D environment with nutrients and noxious distribution. Detailed study on bacterial chemotatic processes with quorum sensing and without quorum sensing are compared. By using quorum sensing mechanisms, artificial bacteria working together can find the nutrient concentration (or global optimum) quickly in the artificial environment. Conclusions Biomimicry of quorum sensing mechanisms using the lifecycle model allows the artificial bacteria endowed with the communication abilities, which are essential to obtain more valuable information to guide their search cooperatively towards the preferred nutrient concentrations. It can also provide an inspiration for designing new swarm intelligence optimization algorithms, which can be used for solving the real-world problems. PMID:23815296

Discordant signaling and autophagy response to fasting in hearts of obese mice: Implications for ischemia tolerance

PubMed Central

Kooren, Joel A.; Parker, Sarah J.; Tucker, Kyle C.; Ravindran, Nandini; Ito, Bruce R.; Huang, Chengqun; Venkatraman, Vidya; Van Eyk, Jennifer E.; Gottlieb, Roberta A.; Mentzer, Robert M.

2016-01-01

Autophagy is regulated by nutrient and energy status and plays an adaptive role during nutrient deprivation and ischemic stress. Metabolic syndrome (MetS) is a hypernutritive state characterized by obesity, dyslipidemia, elevated fasting blood glucose levels, and insulin resistance. It has also been associated with impaired autophagic flux and larger-sized infarcts. We hypothesized that diet-induced obesity (DIO) affects nutrient sensing, explaining the observed cardiac impaired autophagy. We subjected male friend virus B NIH (FVBN) mice to a high-fat diet, which resulted in increased weight gain, fat deposition, hyperglycemia, insulin resistance, and larger infarcts after myocardial ischemia-reperfusion. Autophagic flux was impaired after 4 wk on a high-fat diet. To interrogate nutrient-sensing pathways, DIO mice were subjected to overnight fasting, and hearts were processed for biochemical and proteomic analysis. Obese mice failed to upregulate LC3-II or to clear p62/SQSTM1 after fasting, although mRNA for LC3B and p62/SQSTM1 were appropriately upregulated in both groups, demonstrating an intact transcriptional response to fasting. Energy- and nutrient-sensing signal transduction pathways [AMPK and mammalian target of rapamycin (mTOR)] also responded appropriately to fasting, although mTOR was more profoundly suppressed in obese mice. Proteomic quantitative analysis of the hearts under fed and fasted conditions revealed broad changes in protein networks involved in oxidative phosphorylation, autophagy, oxidative stress, protein homeostasis, and contractile machinery. In many instances, the fasting response was quite discordant between lean and DIO mice. Network analysis implicated the peroxisome proliferator-activated receptor and mTOR regulatory nodes. Hearts of obese mice exhibited impaired autophagy, altered proteome, and discordant response to nutrient deprivation. PMID:27199111

Discordant signaling and autophagy response to fasting in hearts of obese mice: Implications for ischemia tolerance.

PubMed

Andres, Allen M; Kooren, Joel A; Parker, Sarah J; Tucker, Kyle C; Ravindran, Nandini; Ito, Bruce R; Huang, Chengqun; Venkatraman, Vidya; Van Eyk, Jennifer E; Gottlieb, Roberta A; Mentzer, Robert M

2016-07-01

Autophagy is regulated by nutrient and energy status and plays an adaptive role during nutrient deprivation and ischemic stress. Metabolic syndrome (MetS) is a hypernutritive state characterized by obesity, dyslipidemia, elevated fasting blood glucose levels, and insulin resistance. It has also been associated with impaired autophagic flux and larger-sized infarcts. We hypothesized that diet-induced obesity (DIO) affects nutrient sensing, explaining the observed cardiac impaired autophagy. We subjected male friend virus B NIH (FVBN) mice to a high-fat diet, which resulted in increased weight gain, fat deposition, hyperglycemia, insulin resistance, and larger infarcts after myocardial ischemia-reperfusion. Autophagic flux was impaired after 4 wk on a high-fat diet. To interrogate nutrient-sensing pathways, DIO mice were subjected to overnight fasting, and hearts were processed for biochemical and proteomic analysis. Obese mice failed to upregulate LC3-II or to clear p62/SQSTM1 after fasting, although mRNA for LC3B and p62/SQSTM1 were appropriately upregulated in both groups, demonstrating an intact transcriptional response to fasting. Energy- and nutrient-sensing signal transduction pathways [AMPK and mammalian target of rapamycin (mTOR)] also responded appropriately to fasting, although mTOR was more profoundly suppressed in obese mice. Proteomic quantitative analysis of the hearts under fed and fasted conditions revealed broad changes in protein networks involved in oxidative phosphorylation, autophagy, oxidative stress, protein homeostasis, and contractile machinery. In many instances, the fasting response was quite discordant between lean and DIO mice. Network analysis implicated the peroxisome proliferator-activated receptor and mTOR regulatory nodes. Hearts of obese mice exhibited impaired autophagy, altered proteome, and discordant response to nutrient deprivation. Copyright © 2016 the American Physiological Society.

Nutrient control of hunger by extrinsic gastrointestinal neurons.

PubMed

Mithieux, Gilles

2013-08-01

The neural sensing of nutrients during food digestion plays a key role in the regulation of hunger. Recent data have emphasized that the extrinsic gastrointestinal nervous system is preponderant in this phenomenon and in its translation to the control of food intake by the central nervous system (CNS). Nutrient sensing by the extrinsic gastrointestinal nervous system may account for the satiation induced by food lipids, the satiety initiated by food protein, and for the rapid benefits of gastric bypass surgeries on both glucose and energy homeostasis. Thus, this recent knowledge provides novel examples of the mechanisms that control food intake and body weight, and this might pave the way for future approaches to the prevention and/or treatment of obesity. Copyright © 2013 Elsevier Ltd. All rights reserved.

Hyperspectral Remote Sensing of New England Coastal Waters to Predict Seagrass Distribution

EPA Science Inventory

The U.S. Environmental Protection Agency is working to improve its ability to quantify and predict aquatic (freshwater, estuarine, marine) ecosystem response and recovery to changing nutrient loads. The objective of this research is to quantify the relationship of nutrients with...

Diatoms morphology and gene expression in turbulence

NASA Astrophysics Data System (ADS)

Iudicone, D.; Amato, A.; Ferrante, M. I.; Ribera, M.

2016-02-01

Diatoms are an ecologically important algal group that prospers in turbulent environments. Despite previous efforts, a general scenario that explains mesocosm and in situ observations is missing. Importantly, according to the present theories, the main effect of microscale turbulence is the increase of nutrient uptake efficiency in nutrient-depleted conditions and for very large cells. We will present evidences from laboratory experiments in nutrient-repleted conditions that chain-forming diatoms sense and respond to turbulence by varying their chain length spectra and tuning their metabolism. Further, we compared growth and gene expressions of small-sized cells in turbulent and in still conditions and found that turbulence sensing activates a series of pathways suggesting a partial metabolic switch in response to agitation. In addition, a long-time exposure experiment showed that when silicates become depleted these same diatoms take up other nutrients differently than in still condition. This implies that in natural environment prolonged turbulence would shape the phytoplankton community structure and succession.

Resveratrol Prevents the Development of Hypertension Programmed by Maternal Plus Post-Weaning High-Fructose Consumption Through Modulation of Oxidative Stress, Nutrient-Sensing Signals, and Gut Microbiota.

PubMed

Tain, You-Lin; Lee, Wei-Chia; Wu, Kay L H; Leu, Steve; Chan, Julie Y H

2018-04-30

High-fructose (HF) intake, oxidative stress, nutrient-sensing signals, and gut microbiota dysbiosis are closely related to the development of hypertension. We investigated whether resveratrol can prevent hypertension induced by maternal plus post-weaning HF diets in adult offspring via the above-mentioned mechanisms. Female Sprague-Dawley rats received either a normal (ND) or 60% high-fructose (HF) diet during gestation and lactation. Male offspring were assigned to five groups (maternal diet/post-weaning diet; n = 8/group): ND/ND, ND/HF, HF/ND, HF/HF, and HF/HF+ Resveratrol. Resveratrol (50 mg/L) was administered in drinking water from weaning to three months of age. We found that HF/HF induced hypertension in adult offspring. Maternal HF diet altered gut microbiota composition in adult offspring, including decreasing the abundance of genera Bacteroides, Dysgonomonas, and Turicibacter, while increasing phylum Verrucomicrobia and Akkermansia muciniphila. Additionally, HF/HF diets increased oxidative stress and decreased renal mRNA expression of Prkaa2, Prkag2, Ppara, Pparb, Ppargc1a, and Sirt4. Resveratrol reduced renal oxidative stress, activated nutrient-sensing signals, modulated gut microbiota, and prevented associated HF/HF-induced programmed hypertension. Targeting oxidative stress, nutrient-sensing signals, and gut microbiota by resveratrol might be a useful therapeutic strategy for treatment of hypertension induced by excessive consumption of fructose in the adult rat offspring. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Remote Sensing the Phytoplankton Seasonal Succession of the Red Sea

PubMed Central

Brewin, Robert J. W.; Stenchikov, Georgiy; Hoteit, Ibrahim

2013-01-01

The Red Sea holds one of the most diverse marine ecosystems, primarily due to coral reefs. However, knowledge on large-scale phytoplankton dynamics is limited. Analysis of a 10-year high resolution Chlorophyll-a (Chl-a) dataset, along with remotely-sensed sea surface temperature and wind, provided a detailed description of the spatiotemporal seasonal succession of phytoplankton biomass in the Red Sea. Based on MODIS (Moderate-resolution Imaging Spectroradiometer) data, four distinct Red Sea provinces and seasons are suggested, covering the major patterns of surface phytoplankton production. The Red Sea Chl-a depicts a distinct seasonality with maximum concentrations seen during the winter time (attributed to vertical mixing in the north and wind-induced horizontal intrusion of nutrient-rich water in the south), and minimum concentrations during the summer (associated with strong seasonal stratification). The initiation of the seasonal succession occurs in autumn and lasts until early spring. However, weekly Chl-a seasonal succession data revealed that during the month of June, consistent anti-cyclonic eddies transfer nutrients and/or Chl-a to the open waters of the central Red Sea. This phenomenon occurs during the stratified nutrient depleted season, and thus could provide an important source of nutrients to the open waters. Remotely-sensed synoptic observations highlight that Chl-a does not increase regularly from north to south as previously thought. The Northern part of the Central Red Sea province appears to be the most oligotrophic area (opposed to southern and northern domains). This is likely due to the absence of strong mixing, which is apparent at the northern end of the Red Sea, and low nutrient intrusion in comparison with the southern end. Although the Red Sea is considered an oligotrophic sea, sporadic blooms occur that reach mesotrophic levels. The water temperature and the prevailing winds control the nutrient concentrations within the euphotic zone and enable the horizontal transportation of nutrients. PMID:23755161

Remote sensing the phytoplankton seasonal succession of the Red Sea.

PubMed

Raitsos, Dionysios E; Pradhan, Yaswant; Brewin, Robert J W; Stenchikov, Georgiy; Hoteit, Ibrahim

2013-01-01

The Red Sea holds one of the most diverse marine ecosystems, primarily due to coral reefs. However, knowledge on large-scale phytoplankton dynamics is limited. Analysis of a 10-year high resolution Chlorophyll-a (Chl-a) dataset, along with remotely-sensed sea surface temperature and wind, provided a detailed description of the spatiotemporal seasonal succession of phytoplankton biomass in the Red Sea. Based on MODIS (Moderate-resolution Imaging Spectroradiometer) data, four distinct Red Sea provinces and seasons are suggested, covering the major patterns of surface phytoplankton production. The Red Sea Chl-a depicts a distinct seasonality with maximum concentrations seen during the winter time (attributed to vertical mixing in the north and wind-induced horizontal intrusion of nutrient-rich water in the south), and minimum concentrations during the summer (associated with strong seasonal stratification). The initiation of the seasonal succession occurs in autumn and lasts until early spring. However, weekly Chl-a seasonal succession data revealed that during the month of June, consistent anti-cyclonic eddies transfer nutrients and/or Chl-a to the open waters of the central Red Sea. This phenomenon occurs during the stratified nutrient depleted season, and thus could provide an important source of nutrients to the open waters. Remotely-sensed synoptic observations highlight that Chl-a does not increase regularly from north to south as previously thought. The Northern part of the Central Red Sea province appears to be the most oligotrophic area (opposed to southern and northern domains). This is likely due to the absence of strong mixing, which is apparent at the northern end of the Red Sea, and low nutrient intrusion in comparison with the southern end. Although the Red Sea is considered an oligotrophic sea, sporadic blooms occur that reach mesotrophic levels. The water temperature and the prevailing winds control the nutrient concentrations within the euphotic zone and enable the horizontal transportation of nutrients.

A vitamin-B2-sensing mechanism that regulates gut protease activity to impact animal’s food behavior and growth

PubMed Central

Qi, Bin; Kniazeva, Marina; Han, Min

2017-01-01

To survive challenging environments, animals acquired the ability to evaluate food quality in the intestine and respond to nutrient deficiencies with changes in food-response behavior, metabolism and development. However, the regulatory mechanisms underlying intestinal sensing of specific nutrients, especially micronutrients such as vitamins, and the connections to downstream physiological responses in animals remain underexplored. We have established a system to analyze the intestinal response to vitamin B2 (VB2) deficiency in Caenorhabditis elegans, and demonstrated that VB2 level critically impacts food uptake and foraging behavior by regulating specific protease gene expression and intestinal protease activity. We show that this impact is mediated by TORC1 signaling through reading the FAD-dependent ATP level. Thus, our study in live animals uncovers a VB2-sensing/response pathway that regulates food-uptake, a mechanism by which a common signaling pathway translates a specific nutrient signal into physiological activities, and the importance of gut microbiota in supplying micronutrients to animals. DOI: http://dx.doi.org/10.7554/eLife.26243.001 PMID:28569665

The Extracellular Calcium-Sensing Receptor in the Intestine: Evidence for Regulation of Colonic Absorption, Secretion, Motility, and Immunity

PubMed Central

Tang, Lieqi; Cheng, Catherine Y.; Sun, Xiangrong; Pedicone, Alexandra J.; Mohamadzadeh, Mansour; Cheng, Sam X.

2016-01-01

Different from other epithelia, the intestinal epithelium has the complex task of providing a barrier impeding the entry of toxins, food antigens, and microbes, while at the same time allowing for the transfer of nutrients, electrolytes, water, and microbial metabolites. These molecules/organisms are transported either transcellularly, crossing the apical and basolateral membranes of enterocytes, or paracellularly, passing through the space between enterocytes. Accordingly, the intestinal epithelium can affect energy metabolism, fluid balance, as well as immune response and tolerance. To help accomplish these complex tasks, the intestinal epithelium has evolved many sensing receptor mechanisms. Yet, their roles and functions are only now beginning to be elucidated. This article explores one such sensing receptor mechanism, carried out by the extracellular calcium-sensing receptor (CaSR). In addition to its established function as a nutrient sensor, coordinating food digestion, nutrient absorption, and regulating energy metabolism, we present evidence for the emerging role of CaSR in the control of intestinal fluid homeostasis and immune balance. An additional role in the modulation of the enteric nerve activity and motility is also discussed. Clearly, CaSR has profound effects on many aspects of intestinal function. Nevertheless, more work is needed to fully understand all functions of CaSR in the intestine, including detailed mechanisms of action and specific pathways involved. Considering the essential roles CaSR plays in gastrointestinal physiology and immunology, research may lead to a translational opportunity for the development of novel therapies that are based on CaSR's unique property of using simple nutrients such as calcium, polyamines, and certain amino acids/oligopeptides as activators. It is possible that, through targeting of intestinal CaSR with a combination of specific nutrients, oral solutions that are both inexpensive and practical may be developed to help in conditioning the gut microenvironment and in maintaining digestive health. PMID:27458380

The Extracellular Calcium-Sensing Receptor in the Intestine: Evidence for Regulation of Colonic Absorption, Secretion, Motility, and Immunity.

PubMed

Tang, Lieqi; Cheng, Catherine Y; Sun, Xiangrong; Pedicone, Alexandra J; Mohamadzadeh, Mansour; Cheng, Sam X

2016-01-01

Different from other epithelia, the intestinal epithelium has the complex task of providing a barrier impeding the entry of toxins, food antigens, and microbes, while at the same time allowing for the transfer of nutrients, electrolytes, water, and microbial metabolites. These molecules/organisms are transported either transcellularly, crossing the apical and basolateral membranes of enterocytes, or paracellularly, passing through the space between enterocytes. Accordingly, the intestinal epithelium can affect energy metabolism, fluid balance, as well as immune response and tolerance. To help accomplish these complex tasks, the intestinal epithelium has evolved many sensing receptor mechanisms. Yet, their roles and functions are only now beginning to be elucidated. This article explores one such sensing receptor mechanism, carried out by the extracellular calcium-sensing receptor (CaSR). In addition to its established function as a nutrient sensor, coordinating food digestion, nutrient absorption, and regulating energy metabolism, we present evidence for the emerging role of CaSR in the control of intestinal fluid homeostasis and immune balance. An additional role in the modulation of the enteric nerve activity and motility is also discussed. Clearly, CaSR has profound effects on many aspects of intestinal function. Nevertheless, more work is needed to fully understand all functions of CaSR in the intestine, including detailed mechanisms of action and specific pathways involved. Considering the essential roles CaSR plays in gastrointestinal physiology and immunology, research may lead to a translational opportunity for the development of novel therapies that are based on CaSR's unique property of using simple nutrients such as calcium, polyamines, and certain amino acids/oligopeptides as activators. It is possible that, through targeting of intestinal CaSR with a combination of specific nutrients, oral solutions that are both inexpensive and practical may be developed to help in conditioning the gut microenvironment and in maintaining digestive health.

O-GlcNAc cycling: Emerging Roles in Development and Epigenetics

PubMed Central

Love, Dona C.; Krause, Michael W.; Hanover, John A.

2010-01-01

The nutrient-sensing hexosamine signaling pathway modulates the levels of O-linked N-acetylglucosamine (O-GlcNAc) on key targets impacting cellular signaling, protein turnover and gene expression. O-GlcNAc cycling may be deregulated in neurodegenerative disease, cancer, and diabetes. Studies in model organisms demonstrate that the O-GlcNAc transferase (OGT/Sxc) is essential for Polycomb group (PcG) repression of the homeotic genes, clusters of genes responsible for the adult body plan. Surprisingly, from flies to man, the O-GlcNAcase (OGA, MGEA5) gene is embedded within the NK cluster, the most evolutionarily ancient of three homeobox gene clusters regulated by PcG repression. PcG repression also plays a key role in maintaining stem cell identity, recruiting the DNA methyltransferase machinery for imprinting, and in X-chromosome inactivation. Intriguingly, the Ogt gene resides near the Xist locus in vertebrates and is subject to regulation by PcG-dependent X-inactivation. OGT is also an enzymatic component of the human dosage compensation complex. These ‘evo-devo’ relationships linking O-GlcNAc cycling to higher order chromatin structure provide insights into how nutrient availability may influence the epigenetic regulation of gene expression. O-GlcNAc cycling at promoters and PcG repression represent concrete mechanisms by which nutritional information may be transmitted across generations in the intra-uterine environment. Thus, the nutrient-sensing hexosamine signaling pathway may be a key contributor to the metabolic deregulation resulting from prenatal exposure to famine, or the ‘vicious cycle’ observed in children of mothers with type-2 diabetes and metabolic disease. PMID:20488252

Catabolite and Oxygen Regulation of Enterohemorrhagic Escherichia coli Virulence.

PubMed

Carlson-Banning, Kimberly M; Sperandio, Vanessa

2016-11-22

The biogeography of the gut is diverse in its longitudinal axis, as well as within specific microenvironments. Differential oxygenation and nutrient composition drive the membership of microbial communities in these habitats. Moreover, enteric pathogens can orchestrate further modifications to gain a competitive advantage toward host colonization. These pathogens are versatile and adept when exploiting the human colon. They expertly navigate complex environmental cues and interkingdom signaling to colonize and infect their hosts. Here we demonstrate how enterohemorrhagic Escherichia coli (EHEC) uses three sugar-sensing transcription factors, Cra, KdpE, and FusR, to exquisitely regulate the expression of virulence factors associated with its type III secretion system (T3SS) when exposed to various oxygen concentrations. We also explored the effect of mucin-derived nonpreferred carbon sources on EHEC growth and expression of virulence genes. Taken together, the results show that EHEC represses the expression of its T3SS when oxygen is absent, mimicking the largely anaerobic lumen, and activates its T3SS when oxygen is available through Cra. In addition, when EHEC senses mucin-derived sugars heavily present in the O-linked and N-linked glycans of the large intestine, virulence gene expression is initiated. Sugars derived from pectin, a complex plant polysaccharide digested in the large intestine, also increased virulence gene expression. Not only does EHEC sense host- and microbiota-derived interkingdom signals, it also uses oxygen availability and mucin-derived sugars liberated by the microbiota to stimulate expression of the T3SS. This precision in gene regulation allows EHEC to be an efficient pathogen with an extremely low infectious dose. Enteric pathogens have to be crafty when interpreting multiple environmental cues to successfully establish themselves within complex and diverse gut microenvironments. Differences in oxygen tension and nutrient composition determine the biogeography of the gut microbiota and provide unique niches that can be exploited by enteric pathogens. EHEC is an enteric pathogen that colonizes the colon and causes outbreaks of bloody diarrhea and hemolytic-uremic syndrome worldwide. It has a very low infectious dose, which requires it to be an extremely effective pathogen. Hence, here we show that EHEC senses multiple sugar sources and oxygen levels to optimally control the expression of its virulence repertoire. This exquisite regulatory control equips EHEC to sense different intestinal compartments to colonize the host. Copyright © 2016 Carlson-Banning and Sperandio.

Regulation of iron transport systems in Enterobacteriaceae in response to oxygen and iron availability.

PubMed

Carpenter, Chandra; Payne, Shelley M

2014-04-01

Iron is an essential nutrient for most bacteria. Depending on the oxygen available in the surrounding environment, iron is found in two distinct forms: ferrous (Fe(II)) or ferric (Fe(III)). Bacteria utilize different transport systems for the uptake of the two different forms of iron. In oxic growth conditions, iron is found in its insoluble, ferric form, and in anoxic growth conditions iron is found in its soluble, ferrous form. Enterobacteriaceae have adapted to transporting the two forms of iron by utilizing the global, oxygen-sensing regulators, ArcA and Fnr to regulate iron transport genes in response to oxygen. Copyright © 2014 Elsevier Inc. All rights reserved.

Synergism between inositol polyphosphates and TOR kinase signaling in nutrient sensing, growth control, and lipid metabolism in Chlamydomonas

USDA-ARS?s Scientific Manuscript database

The networks that govern carbon metabolism and control intracellular carbon partitioning in photosynthetic cells are poorly understood. Target of rapamycin (TOR) kinase is a conserved growth regulator that integrates nutrient signals and modulates cell growth in eukaryotes, though the TOR signaling ...

The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing

PubMed Central

Bartok, Osnat; Teesalu, Mari; Ashwall-Fluss, Reut; Pandey, Varun; Hanan, Mor; Rovenko, Bohdana M; Poukkula, Minna; Havula, Essi; Moussaieff, Arieh; Vodala, Sadanand; Nahmias, Yaakov; Kadener, Sebastian; Hietakangas, Ville

2015-01-01

Nutrient sensing pathways adjust metabolism and physiological functions in response to food intake. For example, sugar feeding promotes lipogenesis by activating glycolytic and lipogenic genes through the Mondo/ChREBP-Mlx transcription factor complex. Concomitantly, other metabolic routes are inhibited, but the mechanisms of transcriptional repression upon sugar sensing have remained elusive. Here, we characterize cabut (cbt), a transcription factor responsible for the repressive branch of the sugar sensing transcriptional network in Drosophila. We demonstrate that cbt is rapidly induced upon sugar feeding through direct regulation by Mondo-Mlx. We found that CBT represses several metabolic targets in response to sugar feeding, including both isoforms of phosphoenolpyruvate carboxykinase (pepck). Deregulation of pepck1 (CG17725) in mlx mutants underlies imbalance of glycerol and glucose metabolism as well as developmental lethality. Furthermore, we demonstrate that cbt provides a regulatory link between nutrient sensing and the circadian clock. Specifically, we show that a subset of genes regulated by the circadian clock are also targets of CBT. Moreover, perturbation of CBT levels leads to deregulation of the circadian transcriptome and circadian behavioral patterns. PMID:25916830

Effect of crop rotation on soil nutrient balance and weediness in soddy podzolic organic farming fields

NASA Astrophysics Data System (ADS)

Zarina, Livija; Zarina, Liga

2017-04-01

The nutrient balance in different crop rotations under organic cropping system has been investigated in Latvia at the Institute of Agricultural Resources and Economics since 2006. Latvia is located in a humid and moderate climatic region where the rainfall exceeds evaporation (soil moisture coefficient > 1) and the soil moisture regime is characteristic with percolation. The average annual precipitation is 670-850 mm. The average temperature varies from -6.7° C in January to 16.5 °C in July. The growing season is 175 - 185 days. The most widespread are podzolic soils and mainly they are present in agricultural fields in all regions of Latvia. In a wider sense the goal of the soil management in organic farming is a creation of the biologically active flora and fauna in the soil by maintaining a high level of soil organic matter which is good for crops nutrient balance. Crop rotation is a central component of organic farming systems and has many benefits, including growth of soil microbial activity, which may increase nutrient availability. The aim of the present study was to calculate nutrient balance for each crop in the rotations and average in each rotation. Taking into account that crop rotations can limit build-up of weeds, additionally within the ERA-net CORE Organic Plus transnational programs supported project PRODIVA the information required for a better utilization of crop diversification for weed management in North European organic arable cropping systems was summarized. It was found that the nutrient balance was influenced by nutrients uptake by biomass of growing crops in crop rotation. The number of weeds in the organic farming fields with crop rotation is dependent on the cultivated crops and the succession of crops in the crop rotation.

Piezoresistive microcantilever based lab-on-a-chip system for detection of macronutrients in the soil

NASA Astrophysics Data System (ADS)

Patkar, Rajul S.; Ashwin, Mamta; Rao, V. Ramgopal

2017-12-01

Monitoring of soil nutrients is very important in precision agriculture. In this paper, we have demonstrated a micro electro mechanical system based lab-on-a-chip system for detection of various soil macronutrients which are available in ionic form K+, NO3-, and H2PO4-. These sensors are highly sensitive piezoresistive silicon microcantilevers coated with a polymer matrix containing methyltridodecylammonium nitrate ionophore/ nitrate ionophore VI for nitrate sensing, 18-crown-6 ether for potassium sensing and Tributyltin chloride for phosphate detection. A complete lab-on-a-chip system integrating a highly sensitive current excited Wheatstone's bridge based portable electronic setup along with arrays of microcantilever devices mounted on a printed circuit board with a liquid flow cell for on the site experimentation for soil test has been demonstrated.

SNF3 as High Affinity Glucose Sensor and Its Function in Supporting the Viability of Candida glabrata under Glucose-Limited Environment.

PubMed

Ng, Tzu Shan; Chew, Shu Yih; Rangasamy, Premmala; Mohd Desa, Mohd N; Sandai, Doblin; Chong, Pei Pei; Than, Leslie Thian Lung

2015-01-01

Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability. It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites. Therefore, nutrient sensing particularly glucose sensing is thought to be crucial in contributing to the development and fitness of the pathogen. This study aimed to elucidate the role of SNF3 (Sucrose Non Fermenting 3) as a glucose sensor and its possible role in contributing to the fitness and survivability of C. glabrata in glucose-limited environment. The SNF3 knockout strain was constructed and subjected to different glucose concentrations to evaluate its growth, biofilm formation, amphotericin B susceptibility, ex vivo survivability and effects on the transcriptional profiling of the sugar receptor repressor (SRR) pathway-related genes. The CgSNF3Δ strain showed a retarded growth in low glucose environments (0.01 and 0.1%) in both fermentation and respiration-preferred conditions but grew well in high glucose concentration environments (1 and 2%). It was also found to be more susceptible to amphotericin B in low glucose environment (0.1%) and macrophage engulfment but showed no difference in the biofilm formation capability. The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine). Overall, the deletion of SNF3 causes significant reduction in the ability of C. glabrata to sense limited surrounding glucose and consequently disrupts its competency to transport and perform the uptake of this critical nutrient. This study highlighted the role of SNF3 as a high affinity glucose sensor and its role in aiding the survivability of C. glabrata particularly in glucose limited environment.

High nutrient availability reduces the diversity and stability of the equine caecal microbiota

PubMed Central

Hansen, Naja C. K.; Avershina, Ekaterina; Mydland, Liv T.; Næsset, Jon A.; Austbø, Dag; Moen, Birgitte; Måge, Ingrid; Rudi, Knut

2015-01-01

Background It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown. Methods Here we address the equine caecal microbiota temporal stability, diversity, and functionality in response to diets with different levels of nutrient availability. Hay (low and slower nutrient availability) versus a mixture of hay and whole oats (high and more rapid nutrient availability) were used as experimental diets. Results We found major effects on the microbiota despite that the caecal pH was far from sub-clinical acidosis. We found that the low nutrient availability diet was associated with a higher level of both diversity and temporal stability of the caecal microbiota than the high nutrient availability diet. These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity. Conclusion Nutrient availability does not only change the composition but also the ecology of the caecal microbiota. PMID:26246403

Dally Proteoglycan Mediates the Autonomous and Nonautonomous Effects on Tissue Growth Caused by Activation of the PI3K and TOR Pathways

PubMed Central

Ferreira, Ana; Milán, Marco

2015-01-01

How cells acquiring mutations in tumor suppressor genes outcompete neighboring wild-type cells is poorly understood. The phosphatidylinositol 3-kinase (PI3K)–phosphatase with tensin homology (PTEN) and tuberous sclerosis complex (TSC)-target of rapamycin (TOR) pathways are frequently activated in human cancer, and this activation is often causative of tumorigenesis. We utilized the Gal4-UAS system in Drosophila imaginal primordia, highly proliferative and growing tissues, to analyze the impact of restricted activation of these pathways on neighboring wild-type cell populations. Activation of these pathways leads to an autonomous induction of tissue overgrowth and to a remarkable nonautonomous reduction in growth and proliferation rates of adjacent cell populations. This nonautonomous response occurs independently of where these pathways are activated, is functional all throughout development, takes place across compartments, and is distinct from cell competition. The observed autonomous and nonautonomous effects on tissue growth rely on the up-regulation of the proteoglycan Dally, a major element involved in modulating the spreading, stability, and activity of the growth promoting Decapentaplegic (Dpp)/transforming growth factor β(TGF-β) signaling molecule. Our findings indicate that a reduction in the amount of available growth factors contributes to the outcompetition of wild-type cells by overgrowing cell populations. During normal development, the PI3K/PTEN and TSC/TOR pathways play a major role in sensing nutrient availability and modulating the final size of any developing organ. We present evidence that Dally also contributes to integrating nutrient sensing and organ scaling, the fitting of pattern to size. PMID:26313758

Using remote sensing to calculate plant available nitrogen needed by crops on swine factory farm sprayfields in North Carolina

NASA Astrophysics Data System (ADS)

Christenson, Elizabeth; Serre, Marc

2015-10-01

North Carolina (NC) is the second largest producer of hogs in the United States with Duplin county, NC having the densest population of hogs in the world. In NC, liquid swine manure is generally stored in open-air lagoons and sprayed onto sprayfields with sprinkler systems to be used as fertilizer for crops. Swine factory farms, termed concentrated animal feeding operations (CAFOs), are regulated by the Department of Environment and Natural Resources (DENR) based on nutrient management plans (NMPs) having balanced plant available nitrogen (PAN). The estimated PAN in liquid manure being sprayed must be less than the estimated PAN needed crops during irrigation. Estimates for PAN needed by crops are dependent on crop and soil types. Objectives of this research were to develop a new, time-efficient method to identify PAN needed by crops on Duplin county sprayfields for years 2010-2014. Using remote sensing data instead of NMP data to identify PAN needed by crops allowed calendar year identification of which crops were grown on sprayfields instead of a five-year range of values. Although permitted data have more detailed crop information than remotely sensed data, identification of PAN needed by crops using remotely sensed data is more time efficient, internally consistent, easily publically accessible, and has the ability to identify annual changes in PAN on sprayfields. Once PAN needed by crops is known, remote sensing can be used to quantify PAN at other spatial scales, such as sub-watershed levels, and can be used to inform targeted water quality monitoring of swine CAFOs.

Automated sensing of hydroponic macronutrients using a computer-controlled system with an array of ion-selective electrodes

USDA-ARS?s Scientific Manuscript database

Hydroponic production systems grow plants without soil, relying on a circulating solution to provide the necessary nutrients. Maintaining an optimum nutrient balance in this solution is important for maximizing crop growth and yield. Particularly in closed hydroponic systems it is important to monit...

Characterization of phospate permease in Fusarium oxysporum f. sp vasinfectum growth, virulence and secondary metabolism

USDA-ARS?s Scientific Manuscript database

Microbial development depends on the effective sensing of environmental signals and appropriate acquisition of nutrients and other molecules critical for the organism’s growth. Levels of nutrients and other molecules within the cell, and those outside of the cell, are usually relayed to the nucleus...

CNS-targets in control of energy and glucose homeostasis.

PubMed

Kleinridders, André; Könner, A Christine; Brüning, Jens C

2009-12-01

The exceeding efforts in understanding the signals initiated by nutrients and hormones in the central nervous system (CNS) to regulate glucose and energy homeostasis have largely revolutionized our understanding of the neurocircuitry in control of peripheral metabolism. The ability of neurons to sense nutrients and hormones and to adopt a coordinated response to these signals is of crucial importance in controlling food intake, energy expenditure, glucose and lipid metabolism. Anatomical lesion experiments, pharmacological inhibition of signaling pathways, and, more recently, the analysis of conditional mouse mutants with modifications of hormone and nutrient signaling in defined neuronal populations have broadened our understanding of these complex neurocircuits. This review summarizes recent findings regarding the role of the CNS in sensing and transmitting nutritional and hormonal signals to control energy and glucose homeostasis and aims to define them as potential novel drug targets for the treatment of obesity and type 2 diabetes mellitus.

Enhanced flavor-nutrient conditioning in obese rats on a high-fat, high-carbohydrate choice diet.

PubMed

Wald, Hallie S; Myers, Kevin P

2015-11-01

Through flavor-nutrient conditioning rats learn to prefer and increase their intake of flavors paired with rewarding, postingestive nutritional consequences. Since obesity is linked to altered experience of food reward and to perturbations of nutrient sensing, we investigated flavor-nutrient learning in rats made obese using a high fat/high carbohydrate (HFHC) choice model of diet-induced obesity (ad libitum lard and maltodextrin solution plus standard rodent chow). Forty rats were maintained on HFHC to induce substantial weight gain, and 20 were maintained on chow only (CON). Among HFHC rats, individual differences in propensity to weight gain were studied by comparing those with the highest proportional weight gain (obesity prone, OP) to those with the lowest (obesity resistant, OR). Sensitivity to postingestive food reward was tested in a flavor-nutrient conditioning protocol. To measure initial, within-meal stimulation of flavor acceptance by post-oral nutrient sensing, first, in sessions 1-3, baseline licking was measured while rats consumed grape- or cherry-flavored saccharin accompanied by intragastric (IG) water infusion. Then, in the next three test sessions they received the opposite flavor paired with 5 ml of IG 12% glucose. Finally, after additional sessions alternating between the two flavor-infusion contingencies, preference was measured in a two-bottle choice between the flavors without IG infusions. HFHC-OP rats showed stronger initial enhancement of intake in the first glucose infusion sessions than CON or HFHC-OR rats. OP rats also most strongly preferred the glucose-paired flavor in the two-bottle choice. These differences between OP versus OR and CON rats suggest that obesity is linked to responsiveness to postoral nutrient reward, consistent with the view that flavor-nutrient learning perpetuates overeating in obesity. Copyright © 2015 Elsevier Inc. All rights reserved.

Hypothalamic roles of mTOR complex I: Integration of nutrient and hormone signals to regulate energy homeostasis

USDA-ARS?s Scientific Manuscript database

Mammalian or mechanistic target of rapamycin (mTOR) senses nutrient, energy, and hormone signals to regulate metabolism and energy homeostasis. mTOR activity in the hypothalamus, which is associated with changes in energy status, plays a critical role in the regulation of food intake and body weight...

Global climatology and variability of potential new production estimated from remote sensing of sea-surface temperature

NASA Technical Reports Server (NTRS)

Dugdale, Richard C.; Wilkerson, Frances P.

1995-01-01

During this project we have collected numerous shipboard data-bases of oceanic nitrate and silicate versus temperature for both equatorial and coastal upwelling regions. These cruises all have accompanying N-15 measurements of new production. The inverse relationships between nutrients and temperatures have been determined and are being used to obtain surface nutrient fields from sea surface temperatures measured remotely by satellite borne sensors- i.e. AVHRR data from NOAA satellites contained in the MCSST data set for the world ocean provided by the University of Miami. The images and data derived from space in this way show the strong seasonal fluctuations and interannual el Nino fluctuations of the nitrate field. the nitrate data has been used to make estimates of new production for the equatorial pacific which are compared with shipboard measurements when available. The importance of silicate as a nutrient driving new production and the ratio of nitrate to silicate has been discovered to be crucial to better understand the causes of new production variability, so we have added these parameters to our study and have begun to make estimates of these for the equatorial Pacific, derived from the weekly averaged sea surface temperatures (SSTs).

Snf1 Phosphorylates Adenylate Cyclase and Negatively Regulates Protein Kinase A-dependent Transcription in Saccharomyces cerevisiae.

PubMed

Nicastro, Raffaele; Tripodi, Farida; Gaggini, Marco; Castoldi, Andrea; Reghellin, Veronica; Nonnis, Simona; Tedeschi, Gabriella; Coccetti, Paola

2015-10-09

In eukaryotes, nutrient availability and metabolism are coordinated by sensing mechanisms and signaling pathways, which influence a broad set of cellular functions such as transcription and metabolic pathways to match environmental conditions. In yeast, PKA is activated in the presence of high glucose concentrations, favoring fast nutrient utilization, shutting down stress responses, and boosting growth. On the contrary, Snf1/AMPK is activated in the presence of low glucose or alternative carbon sources, thus promoting an energy saving program through transcriptional activation and phosphorylation of metabolic enzymes. The PKA and Snf1/AMPK pathways share common downstream targets. Moreover, PKA has been reported to negatively influence the activation of Snf1/AMPK. We report a new cross-talk mechanism with a Snf1-dependent regulation of the PKA pathway. We show that Snf1 and adenylate cyclase (Cyr1) interact in a nutrient-independent manner. Moreover, we identify Cyr1 as a Snf1 substrate and show that Snf1 activation state influences Cyr1 phosphorylation pattern, cAMP intracellular levels, and PKA-dependent transcription. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Reduced Glucose Sensation Can Increase the Fitness of Saccharomyces cerevisiae Lacking Mitochondrial DNA

PubMed Central

Akdoğan, Emel; Tardu, Mehmet; Garipler, Görkem; Baytek, Gülkız; Kavakli, İ. Halil; Dunn, Cory D.

2016-01-01

Damage to the mitochondrial genome (mtDNA) can lead to diseases for which there are no clearly effective treatments. Since mitochondrial function and biogenesis are controlled by the nutrient environment of the cell, it is possible that perturbation of conserved, nutrient-sensing pathways may successfully treat mitochondrial disease. We found that restricting glucose or otherwise reducing the activity of the protein kinase A (PKA) pathway can lead to improved proliferation of Saccharomyces cerevisiae cells lacking mtDNA and that the transcriptional response to mtDNA loss is reduced in cells with diminished PKA activity. We have excluded many pathways and proteins from being individually responsible for the benefits provided to cells lacking mtDNA by PKA inhibition, and we found that robust import of mitochondrial polytopic membrane proteins may be required in order for cells without mtDNA to receive the full benefits of PKA reduction. Finally, we have discovered that the transcription of genes involved in arginine biosynthesis and aromatic amino acid catabolism is altered after mtDNA damage. Our results highlight the potential importance of nutrient detection and availability on the outcome of mitochondrial dysfunction. PMID:26751567

[Nutrient sensing by the gastro-intestinal nervous system and control of energy homeostasis].

PubMed

Gilles, Mithieux

2015-01-01

The gastrointestinal nerves are crucial in the sensing of nutrients and hormones and its translation in terms of control of food intake. Major macronutrients like glucose and proteins are sensed by the extrinsic nerves located around the portal vein walls, which signal to the brain and account for the satiety phenomenon they promote. Glucose is sensed in the portal vein by neurons expressing the glucose receptor SGLT3, which activates the main regions of the brain involved in the control of food intake. Proteins indirectly act on food intake by inducing intestinal gluconeogenesis and its sensing by the portal glucose sensor. The mechanism involves a prior antagonism by peptides of the μ-opioid receptors present in the portal vein nervous system and a reflex arc with the brain inducing intestinal gluconeogenesis. In a comparable manner, short chain fatty acids produced from soluble fibers act via intestinal gluconeogenesis to exert anti-obesity and anti-diabetic effects. In the case of propionate, the mechanism involves a prior activation of the free fatty acid receptor FFAR3 present in the portal nerves and a reflex arc initiating intestinal gluconeogenesis. © Société de Biologie, 2016.

Grafting: A Technique to Modify Ion Accumulation in Horticultural Crops

PubMed Central

Nawaz, Muhammad A.; Imtiaz, Muhammad; Kong, Qiusheng; Cheng, Fei; Ahmed, Waqar; Huang, Yuan; Bie, Zhilong

2016-01-01

Grafting is a centuries-old technique used in plants to obtain economic benefits. Grafting increases nutrient uptake and utilization efficiency in a number of plant species, including fruits, vegetables, and ornamentals. Selected rootstocks of the same species or close relatives are utilized in grafting. Rootstocks absorb more water and ions than self-rooted plants and transport these water and ions to the aboveground scion. Ion uptake is regulated by a complex communication mechanism between the scion and rootstock. Sugars, hormones, and miRNAs function as long-distance signaling molecules and regulate ion uptake and ion homeostasis by affecting the activity of ion transporters. This review summarizes available information on the effect of rootstock on nutrient uptake and utilization and the mechanisms involved. Information on specific nutrient-efficient rootstocks for different crops of commercial importance is also provided. Several other important approaches, such as interstocking (during double grafting), inarching, use of plant-growth-promoting rhizobacteria, use of arbuscular mycorrhizal fungi, use of plant growth substances (e.g., auxin and melatonin), and use of genetically engineered rootstocks and scions (transgrafting), are highlighted; these approaches can be combined with grafting to enhance nutrient uptake and utilization in commercially important plant species. Whether the rootstock and scion affect each other's soil microbiota and their effect on the nutrient absorption of rootstocks remain largely unknown. Similarly, the physiological and molecular bases of grafting, crease formation, and incompatibility are not fully identified and require investigation. Grafting in horticultural crops can help reveal the basic biology of grafting, the reasons for incompatibility, sensing, and signaling of nutrients, ion uptake and transport, and the mechanism of heavy metal accumulation and restriction in rootstocks. Ion transporter and miRNA-regulated nutrient studies have focused on model and non-grafted plants, and information on grafted plants is limited. Such information will improve the development of nutrient-efficient rootstocks. PMID:27818663

Structural basis for leucine sensing by the Sestrin2-mTORC1 pathway

DOE PAGES

Saxton, Robert A.; Knockenhauer, Kevin E.; Wolfson, Rachel L.; ...

2015-11-19

Eukaryotic cells coordinate growth with the availability of nutrients through mTOR complex 1 (mTORC1), a master growth regulator. Leucine is of particular importance and activates mTORC1 via the Rag GTPases and their regulators GATOR1 and GATOR2. Sestrin2 interacts with GATOR2 and is a leucine sensor. We present the 2.7-Å crystal structure of Sestrin2 in complex with leucine. Leucine binds through a single pocket that coordinates its charged functional groups and confers specificity for the hydrophobic side chain. A loop encloses leucine and forms a lid-latch mechanism required for binding. A structure-guided mutation in Sestrin2 that decreases its affinity for leucinemore » leads to a concomitant increase in the leucine concentration required for mTORC1 activation in cells. Lastly, these results provide a structural mechanism of amino acid sensing by the mTORC1 pathway.« less

Structural basis for leucine sensing by the Sestrin2-mTORC1 pathway

PubMed Central

Saxton, Robert A.; Knockenhauer, Kevin E.; Wolfson, Rachel L.; Chantranupong, Lynne; Pacold, Michael E.; Wang, Tim; Schwartz, Thomas U.; Sabatini, David M.

2015-01-01

Eukaryotic cells coordinate growth with the availability of nutrients through mTOR complex 1 (mTORC1), a master growth regulator. Leucine is of particular importance and activates mTORC1 via the Rag GTPases and their regulators GATOR1 and GATOR2. Sestrin2 interacts with GATOR2 and is a leucine sensor. We present the 2.7-Å crystal structure of Sestrin2 in complex with leucine. Leucine binds through a single pocket that coordinates its charged functional groups and confers specificity for the hydrophobic side chain. A loop encloses leucine and forms a lid-latch mechanism required for binding. A structure-guided mutation in Sestrin2 that decreases its affinity for leucine leads to a concomitant increase in the leucine concentration required for mTORC1 activation in cells. These results provide a structural mechanism of amino acid sensing by the mTORC1 pathway. PMID:26586190

Managing urban nutrient biogeochemistry for sustainable urbanization.

PubMed

Lin, Tao; Gibson, Valerie; Cui, Shenghui; Yu, Chang-Ping; Chen, Shaohua; Ye, Zhilong; Zhu, Yong-Guan

2014-09-01

Urban ecosystems are unique in the sense that human activities are the major drivers of biogeochemical processes. Along with the demographic movement into cities, nutrients flow towards the urban zone (nutrient urbanization), causing the degradation of environmental quality and ecosystem health. In this paper, we summarize the characteristics of nutrient cycling within the urban ecosystem compared to natural ecosystems. The dynamic process of nutrient urbanization is then explored taking Xiamen city, China, as an example to examine the influence of rapid urbanization on food sourced nitrogen and phosphorus metabolism. Subsequently, the concept of a nutrient footprint and calculation method is introduced from a lifecycle perspective. Finally, we propose three system approaches to mend the broken biogeochemical cycling. Our study will contribute to a holistic solution which achieves synergies between environmental quality and food security, by integrating technologies for nutrient recovery and waste reduction. Copyright © 2014 Elsevier Ltd. All rights reserved.

Sensor kinase KinB and its pathway-associated key factors sense the signal of nutrition starvation in sporulation of Bacillus subtilis.

PubMed

Liu, Weipeng; He, Zeying; Gao, Feng; Yan, Jinyuan; Huang, Xiaowei

2018-01-03

Bacillus subtilis responds to environmental stress cues and develops endospores for survival. In the process of endospore formation, sporulation initiation is a vital stage and this stage is governed by autophosphorylation of the sensor histidine kinases. The second major sensor kinase KinB perceives the intracellular changes of GTP and ATP during sporulation. However, determination of the environmental signals as well as its related signaling pathway of KinB requires further elucidation. Our current study found that, contrary to the sporulation failure induced by ΔkinA in the nutrient-rich 2× SG medium, the sensor kinase KinB sensed the environmental cues in the nutrient-poor MM medium. Two other membrane proteins, KapB and KbaA, also responded similarly to the same external signal as KinB. Both KapB and KbaA acted upstream of KinB, but they exerted their regulation upon KinB independently. Furthermore, we demonstrated that both the SH3 domain and the α-helix structure in KapB are required for sensing or transducing the signal of sporulation initiation. Collectively, our work here supplied the direct evidences that KinB and its pathway sense the external signal of nutrient starvation in MM medium, and further analyzes the interrelationship among KinB, KbaA, and KapB. © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

Drainage and fertilization effects on nutrient availability in an ombrotrophic peatland.

PubMed

Wang, Meng; Talbot, Julie; Moore, Tim R

2018-04-15

Nutrient availability is an important control on the vegetation distribution, productivity and functioning of peatland ecosystems and we examined spatial and temporal patterns of nutrient availability through ion exchange at Mer Bleue bog, southeast Ontario, Canada. We installed ion exchange probes at 5-15cm for 4weeks and determined nutrient sorption at undisturbed sites as well as those affected by nitrogen (N), phosphorus (P), potassium (K) fertilization and drainage. Under undisturbed conditions, the bog had very small amount of available nutrients, especially N (ammonium>nitrate) and P, and exhibited small variations in nutrient availability during the growing season (May to October). The increase in NPK availability upon fertilization was short-lived over the season and the stoichiometry of available NPK captured by the probes was mismatched with the vegetation. The increase in nutrient availability with drainage was confounded by substantial changes in vegetation. We compare these results with data from other Canadian bogs and fens to provide baseline data on nutrient availability in peatlands. Copyright © 2017 Elsevier B.V. All rights reserved.

The relative importance of vertical soil nutrient heterogeneity, and mean and depth-specific soil nutrient availabilities for tree species richness in tropical forests and woodlands.

PubMed

Shirima, Deo D; Totland, Ørjan; Moe, Stein R

2016-11-01

The relative importance of resource heterogeneity and quantity on plant diversity is an ongoing debate among ecologists, but we have limited knowledge on relationships between tree diversity and heterogeneity in soil nutrient availability in tropical forests. We expected tree species richness to be: (1) positively related to vertical soil nutrient heterogeneity; (2) negatively related to mean soil nutrient availability; and (3) more influenced by nutrient availability in the upper than lower soil horizons. Using a data set from 60, 20 × 40-m plots in a moist forest, and 126 plots in miombo woodlands in Tanzania, we regressed tree species richness against vertical soil nutrient heterogeneity, both depth-specific (0-15, 15-30, and 30-60 cm) and mean soil nutrient availability, and soil physical properties, with elevation and measures of anthropogenic disturbance as co-variables. Overall, vertical soil nutrient heterogeneity was the best predictor of tree species richness in miombo but, contrary to our prediction, the relationships between tree species richness and soil nutrient heterogeneity were negative. In the moist forest, mean soil nutrient availability explained considerable variations in tree species richness, and in line with our expectations, these relationships were mainly negative. Soil nutrient availability in the top soil layer explained more of the variation in tree species richness than that in the middle and lower layers in both vegetation types. Our study shows that vertical soil nutrient heterogeneity and mean availability can influence tree species richness at different magnitudes in intensively utilized tropical vegetation types.

Characterization and analysis of pasture degradation in Rondonia using remote sensing

NASA Astrophysics Data System (ADS)

Numata, Izaya

2006-04-01

Although pasture degradation has been a regional concern in Amazonian ecosystems, our ability to characterize and monitor pasture degradation under different environmental and human-related conditions is still limited. This dissertation evaluated pasture degradation as it varied due to environmental and human factors across different scales by combining field measures, ancillary data, and remote sensing. To better understand the link between pasture nutrients and soil chemistry, samples were analyzed in the laboratory demonstrating that pasture soil fertility and grass nutrients varied significantly according to soil order. Pastures established on Alfisols, nutrient-rich soils, had higher levels of Phosphorus in soil and grass compared to pastures established on Oxisols and Ultisols. To evaluate remote sensing measures of pasture biophysical properties related to pasture degradation, remote sensing analysis focused on a variety of sensors that provide a range in spatial, spectral and temporal scales, including Landsat Thematic Mapper (TM), a field spectrometer, Hyperion, and the Moderate Resolution Imaging Spectroradiometer (MODIS). Of the measures derived from Landsat, degraded pastures were best characterized by high non-photosynthetic vegetation (NPV) and low shade fractions, while pastures with high biomass were characterized by high green vegetation and low NPV fractions. Absorption features calculated from hyperspectral spectra collected in the field, including water and ligno-cellulose absorption depth and area, provided the best estimates of field grass measures. Temporal MODIS Normalized Difference Vegetation Index (NDVI) data were used to characterize changes in pasture quality across the region and through time. Degraded pastures were characterized by low temporal NDVI variation and occurred in dry or very wet climate conditions and on nutrient poor soils. Productive pastures were characterized by high temporal NDVI variation, were predominantly found more in the central part of the state, and were located in areas with milder climate conditions and relatively more fertile soils. As a general trend of regional pasture change in Rondonia, the proportions of productive pastures decreased and degraded pastures increased as pastures aged. The results obtained in this dissertation will contribute to understanding pasture sustainability needs for the future of Rondonia and provide the first step in monitoring pasture degradation in the Amazon using remote sensing.

Sensing of glucose in the brain.

PubMed

Thorens, Bernard

2012-01-01

The brain, and in particular the hypothalamus and brainstem, have been recognized for decades as important centers for the homeostatic control of feeding, energy expenditure, and glucose homeostasis. These structures contain neurons and neuronal circuits that may be directly or indirectly activated or inhibited by glucose, lipids, or amino acids. The detection by neurons of these nutrient cues may become deregulated, and possibly cause metabolic diseases such as obesity and diabetes. Thus, there is a major interest in identifying these neurons, how they respond to nutrients, the neuronal circuits they form, and the physiological function they control. Here I will review some aspects of glucose sensing by the brain. The brain is responsive to both hyperglycemia and hypoglycemia, and the glucose sensing cells involved are distributed in several anatomical sites that are connected to each other. These eventually control the activity of the sympathetic or parasympathetic nervous system, which regulates the function of peripheral organs such as liver, white and brown fat, muscle, and pancreatic islets alpha and beta cells. There is now evidence for an extreme diversity in the sensing mechanisms used, and these will be reviewed.

Nutrition of mangroves.

PubMed

Reef, Ruth; Feller, Ilka C; Lovelock, Catherine E

2010-09-01

Mangrove forests dominate the world's tropical and subtropical coastlines. Similar to other plant communities, nutrient availability is one of the major factors influencing mangrove forest structure and productivity. Many mangrove soils have extremely low nutrient availability, although nutrient availability can vary greatly among and within mangrove forests. Nutrient-conserving processes in mangroves are well developed and include evergreeness, resorption of nutrients prior to leaf fall, the immobilization of nutrients in leaf litter during decomposition, high root/shoot ratios and the repeated use of old root channels. Both nitrogen-use efficiency and nutrient resorption efficiencies in mangroves are amongst the highest recorded for angiosperms. A complex range of interacting abiotic and biotic factors controls the availability of nutrients to mangrove trees, and mangroves are characteristically plastic in their ability to opportunistically utilize nutrients when these become available. Nitrogen and phosphorus have been implicated as the nutrients most likely to limit growth in mangroves. Ammonium is the primary form of nitrogen in mangrove soils, in part as a result of anoxic soil conditions, and tree growth is supported mainly by ammonium uptake. Nutrient enrichment is a major threat to marine ecosystems. Although mangroves have been proposed to protect the marine environment from land-derived nutrient pollution, nutrient enrichment can have negative consequences for mangrove forests and their capacity for retention of nutrients may be limited.

Mitochondrial protein acetylation mediates nutrient sensing of mitochondrial protein synthesis and mitonuclear protein balance.

PubMed

Di Domenico, Antonella; Hofer, Annette; Tundo, Federica; Wenz, Tina

2014-11-01

Changes in nutrient supply require global metabolic reprogramming to optimize the utilization of the nutrients. Mitochondria as a central component of the cellular metabolism play a key role in this adaptive process. Since mitochondria harbor their own genome, which encodes essential enzymes, mitochondrial protein synthesis is a determinant of metabolic adaptation. While regulation of cytoplasmic protein synthesis in response to metabolic challenges has been studied in great detail, mechanisms which adapt mitochondrial translation in response to metabolic challenges remain elusive. Our results suggest that the mitochondrial acetylation status controlled by Sirt3 and its proposed opponent GCN5L1 is an important regulator of the metabolic adaptation of mitochondrial translation. Moreover, both proteins modulate regulators of cytoplasmic protein synthesis as well as the mitonuclear protein balance making Sirt3 and GCN5L1 key players in synchronizing mitochondrial and cytoplasmic translation. Our results thereby highlight regulation of mitochondrial translation as a novel component in the cellular nutrient sensing scheme and identify mitochondrial acetylation as a new regulatory principle for the metabolic competence of mitochondrial protein synthesis. © 2014 International Union of Biochemistry and Molecular Biology.

Long- and short-term changes in nutrient availability following commercial sawlog harvest via cable logging

Treesearch

Jennifer Knoepp; Wayne Swank; Bruce L. Haines

2014-01-01

Soil nutrient availability often limits forest productivity and soils have considerable variation in their ability to supply nutrients. Most southern Appalachian forests are minimally managed with no fertilizer inputs or routine thinning regime. Nutrient availability is regulated by atmospheric inputs and the internal cycling of nutrients through such processes as...

Upper intestinal lipids regulate energy and glucose homeostasis.

PubMed

Cheung, Grace W C; Kokorovic, Andrea; Lam, Tony K T

2009-09-01

Upon the entry of nutrients into the small intestine, nutrient sensing mechanisms are activated to allow the body to adapt appropriately to the incoming nutrients. To date, mounting evidence points to the existence of an upper intestinal lipid-induced gut-brain neuronal axis to regulate energy homeostasis. Moreover, a recent discovery has also revealed an upper intestinal lipid-induced gut-brain-liver neuronal axis involved in the regulation of glucose homeostasis. In this mini-review, we will focus on the mechanisms underlying the activation of these respective neuronal axes by upper intestinal lipids.

Monitoring Phenology as Indicator for Timing of Nutrient Inputs in Northern Gulf Watersheds

NASA Technical Reports Server (NTRS)

Ross, Kenton W.; Spiering, Bruce A.; Kalcic, Maria T.

2009-01-01

Nutrient over-enrichment defined by the U.S. Environmental Protection Agency as the anthropogenic addition of nutrients, in addition to any natural processes, causing adverse effects or impairments to the beneficial uses of a water body has been identified as one of the most significant environmental problems facing sensitive estuaries and coastal waters. Understanding the timing of nutrient inputs into those waters through remote sensing observables helps define monitoring and mitigation strategies. Remotely sensed data products can trace both forcings and effects of the nutrient system from landscape to estuary. This project is focused on extracting nutrient information from the landscape. The timing of nutrients entering coastal waters from the land boundary is greatly influenced by hydrologic processes, but can also be affected by the timing of nutrient additions across the landscape through natural or anthropogenic means. Non-point source nutrient additions to watersheds are often associated with specific seasonal cycles, such as decomposition of organic materials in fall and winter or addition of fertilizers to crop lands in the spring. These seasonal cycles or phenology may in turn be observed through the use of satellite sensors. Characterization of the phenology of various land cover types may be of particular interest in Gulf of Mexico estuarine systems with relatively short pathways between intensively managed systems and the land/estuarine boundary. The objective of this study is to demonstrate the capability of monitoring phenology of specific classes of land, such as agriculture and managed timberlands, at a refined watershed level. The extraction of phenological information from the Moderate Resolution Imaging Spectroradiometer (MODIS) data record is accomplished using analytical tools developed for NASA at Stennis Space Center: the Time Series Product Tool and the Phenological Parameters Estimation Tool. MODIS reflectance data (product MOD09) were used to compute the Normalized Difference Vegetation Index, which is sensitive to changes in vegetation canopies. The project team is working directly with the Mississippi Department of Environmental Quality to understand end-user requirements for this type of information product. Initial focus areas are identification of time frames for pre-plant fertilizer applications (prior to start of season), side-dress fertilizer applications (during rapid green-up), and periods of plant decomposition (during and after senescence). Prototypical maps of phenological stages related to these time frames have been generated for watersheds in the northern Gulf of Mexico. Where feasible, these maps have been compared to existing in situ nutrient monitoring data, but the in situ data is temporally sparse (monthly frequency or less), which makes interpretation challenging. Future work will include integrating effects of rainfall and seeking couplings with estuarine remote sensing.

Long term growth responses of loblolly pine to optimal nutrient and water resource availability

Treesearch

Timothy J. Albaugh; H. Lee Allen; Phillip M. Dougherty; Kurt H. Johnsen

2004-01-01

A factorial combination of four treatments (control (CW), optimal growing season water availability (IW), optimum nutrient availability (FW), and combined optimum water and nutrient availability (FIW)) in four replications were initiated in an 8-year- old Pinus taeda stand growing on a droughty, nutrient-poor, sandy site in Scotland County, NC and...

[Use of Remote Sensing for Crop and Soil Analysis

NASA Technical Reports Server (NTRS)

Johannsen, Chris J.

1997-01-01

The primary agricultural objective of this research is to determine what soil and crop information can be verified from remotely sensed images during the growing season. Specifically: (1) Elements of crop stress due to drought, weeds, disease and nutrient deficiencies will be documented with ground truth over specific agricultural sites and (2) Use of remote sensing with GPS and GIS technology for providing a safe and environmentally friendly application of fertilizers and chemicals will be documented.

Brain glucose sensing, counterregulation, and energy homeostasis.

PubMed

Marty, Nell; Dallaporta, Michel; Thorens, Bernard

2007-08-01

Neuronal circuits in the central nervous system play a critical role in orchestrating the control of glucose and energy homeostasis. Glucose, beside being a nutrient, is also a signal detected by several glucose-sensing units that are located at different anatomical sites and converge to the hypothalamus to cooperate with leptin and insulin in controlling the melanocortin pathway.

Nutrient availability at Mer Bleue bog measured by PRSTM probes

NASA Astrophysics Data System (ADS)

Wang, M.; Moore, T. R.; Talbot, J.

2015-12-01

Bogs, covering ~0.7 million km2 in Canada, store a large amount of C and N. As nutrient deficient ecosystems, it's critical to examine the nutrient availabilities and seasonal dynamics. We used Plant Root Simulators (PRSTM) at Mer Bleue bog to provide some baseline data on nutrient availability and its variability. In particular, we focused on ammonium, nitrate, phosphate, calcium, magnesium and potassium, iron, sulphate and aluminum. We placed PRS probes at a depth of 5 - 15 cm in pristine plots and plots with long term N, P and K fertilization for 4 weeks and determined the availability of these nutrients, from spring through to fall. Probes were also placed beneath the water table in hummock and hollow microtopography and along a transect including part of the bog which had been drained through the creation of a ditch 80 years ago. The result showed that there was limited available ammonium, nitrate and phosphate in the bog, the seasonal variation of nutrient availabilities probably due to mineralization, an increase in the availability of some nutrients between different water table depths or as a result of drainage, and the relative availability of nutrients compared to the input from fertilization. We suggest that PRS probes could be a useful tool to examine nutrient availability and dynamics in wetlands, with careful consideration of installing condition, for example, proper exposure period, depth relative to water table etc.

Nitrate Transport, Sensing, and Responses in Plants.

PubMed

O'Brien, José A; Vega, Andrea; Bouguyon, Eléonore; Krouk, Gabriel; Gojon, Alain; Coruzzi, Gloria; Gutiérrez, Rodrigo A

2016-06-06

Nitrogen (N) is an essential macronutrient that affects plant growth and development. N is an important component of chlorophyll, amino acids, nucleic acids, and secondary metabolites. Nitrate is one of the most abundant N sources in the soil. Because nitrate and other N nutrients are often limiting, plants have developed sophisticated mechanisms to ensure adequate supply of nutrients in a variable environment. Nitrate is absorbed in the root and mobilized to other organs by nitrate transporters. Nitrate sensing activates signaling pathways that impinge upon molecular, metabolic, physiological, and developmental responses locally and at the whole plant level. With the advent of genomics technologies and genetic tools, important advances in our understanding of nitrate and other N nutrient responses have been achieved in the past decade. Furthermore, techniques that take advantage of natural polymorphisms present in divergent individuals from a single species have been essential in uncovering new components. However, there are still gaps in our understanding of how nitrate signaling affects biological processes in plants. Moreover, we still lack an integrated view of how all the regulatory factors identified interact or crosstalk to orchestrate the myriad N responses plants typically exhibit. In this review, we provide an updated overview of mechanisms by which nitrate is sensed and transported throughout the plant. We discuss signaling components and how nitrate sensing crosstalks with hormonal pathways for developmental responses locally and globally in the plant. Understanding how nitrate impacts on plant metabolism, physiology, and growth and development in plants is key to improving crops for sustainable agriculture. Copyright © 2016 The Author. Published by Elsevier Inc. All rights reserved.

Plant response to nutrient availability across variable bedrock geologies

USGS Publications Warehouse

Castle, S.C.; Neff, J.C.

2009-01-01

We investigated the role of rock-derived mineral nutrient availability on the nutrient dynamics of overlying forest communities (Populus tremuloides and Picea engelmanni-Abies lasiocarpa v. arizonica) across three parent materials (andesite, limestone, and sandstone) in the southern Rocky Mountains of Colorado. Broad geochemical differences were observed between bedrock materials; however, bulk soil chemistries were remarkably similar between the three different sites. In contrast, soil nutrient pools were considerably different, particularly for P, Ca, and Mg concentrations. Despite variations in nutrient stocks and nutrient availability in soils, we observed relatively inflexible foliar concentrations and foliar stoichiometries for both deciduous and coniferous species. Foliar nutrient resorption (P and K) in the deciduous species followed patterns of nutrient content across substrate types, with higher resorption corresponding to lower bedrock concentrations. Work presented here indicates a complex plant response to available soil nutrients, wherein plant nutrient use compensates for variations in supply gradients and results in the maintenance of a narrow range in foliar stoichiometry. ?? 2008 Springer Science+Business Media, LLC.

Oral sensations and secretions.

PubMed

Running, Cordelia A

2018-04-10

Sensations experienced in the mouth influence food choices, both immediately and in the long term. Such sensations are themselves influenced by experience with flavors, the chemical environment of the mouth, genetics of receptors for flavors, and individual behavior in the chewing of food. Gustation, the sense of taste, yields information about nutrients, influences palatability, and feeds into the human body's preparation to receive those nutrients. Olfaction, the sense of smell, contributes enormously to defining and identifying food flavors (and is experienced even after placing food inside the mouth). Another vital component of food flavor is texture, which contributes to palatability, especially if a food's texture violates a person's expectations. Next, chemesthesis is the sense of chemically induced irritancy and temperature, for example spiciness and stinging. All of these sensations are potentially modified by saliva, the chemical and physical media of the mouth. As a person experiences the culmination of these oral sensations, modified through an individual's own unique saliva, the flavors in turn influence both what and how a person eats. Copyright © 2018 Elsevier Inc. All rights reserved.

Multiple Transceptors for Macro- and Micro-Nutrients Control Diverse Cellular Properties Through the PKA Pathway in Yeast: A Paradigm for the Rapidly Expanding World of Eukaryotic Nutrient Transceptors Up to Those in Human Cells.

PubMed

Steyfkens, Fenella; Zhang, Zhiqiang; Van Zeebroeck, Griet; Thevelein, Johan M

2018-01-01

The nutrient composition of the medium has dramatic effects on many cellular properties in the yeast Saccharomyces cerevisiae . In addition to the well-known specific responses to starvation for an essential nutrient, like nitrogen or phosphate, the presence of fermentable sugar or a respirative carbon source leads to predominance of fermentation or respiration, respectively. Fermenting and respiring cells also show strong differences in other properties, like storage carbohydrate levels, general stress tolerance and cellular growth rate. However, the main glucose repression pathway, which controls the switch between respiration and fermentation, is not involved in control of these properties. They are controlled by the protein kinase A (PKA) pathway. Addition of glucose to respiring yeast cells triggers cAMP synthesis, activation of PKA and rapid modification of its targets, like storage carbohydrate levels, general stress tolerance and growth rate. However, starvation of fermenting cells in a glucose medium for any essential macro- or micro-nutrient counteracts this effect, leading to downregulation of PKA and its targets concomitant with growth arrest and entrance into G0. Re-addition of the lacking nutrient triggers rapid activation of the PKA pathway, without involvement of cAMP as second messenger. Investigation of the sensing mechanism has revealed that the specific high-affinity nutrient transporter(s) induced during starvation function as transporter-receptors or transceptors for rapid activation of PKA upon re-addition of the missing substrate. In this way, transceptors have been identified for amino acids, ammonium, phosphate, sulfate, iron, and zinc. We propose a hypothesis for regulation of PKA activity by nutrient transceptors to serve as a conceptual framework for future experimentation. Many properties of transceptors appear to be similar to those of classical receptors and nutrient transceptors may constitute intermediate forms in the development of receptors from nutrient transporters during evolution. The nutrient-sensing transceptor system in yeast for activation of the PKA pathway has served as a paradigm for similar studies on candidate nutrient transceptors in other eukaryotes and we succinctly discuss the many examples of transceptors that have already been documented in other yeast species, filamentous fungi, plants, and animals, including the examples in human cells.

Biology of eating behavior in obesity.

PubMed

Schwartz, Gary J

2004-11-01

Understanding normal and dysfunctional energy regulation and body weight regulation requires neural evaluation of the signals involved in the control of food intake within a meal, as well as signals related to the availability of stored fuels. Work from our laboratory has focused on peripheral and central nervous system studies of behavior and physiology designed to improve our understanding of the role of gut-brain communication in the control of food intake and energy homeostasis. Gastrointestinal administration of nutrients reduces subsequent meal size, suggesting a potent role for peripheral nutrient sensing in the negative feedback control of ingestion. Vagal afferent nerves supply gastrointestinal sites stimulated during food intake, and these nerves are responsive to mechanical and nutrient chemical properties of ingested food. In addition, the presence of nutrients in these gastrointestinal sites stimulates the release of peptides that affect energy intake. These gut peptides also modulate the activity of peripheral gastrointestinal sensory nerves in ways that may contribute to their effects on food intake. In the central nervous system, adiposity hormones and their downstream mediators have been shown to work at both hindbrain and forebrain sites to affect food intake and metabolism. Importantly, recent data has shown that adiposity hormones acting in the brain increase the behavioral and neural potency of feeding inhibitory gastrointestinal stimuli. These data support the suggestion that insensitivity to adiposity hormones in obesity may be characterized by alterations in their ability to modulate the neural processing of food signals important in determining how much food is consumed during a meal.

Sweet taste receptor in the hypothalamus: a potential new player in glucose sensing in the hypothalamus.

PubMed

Kohno, Daisuke

2017-07-01

The hypothalamic feeding center plays an important role in energy homeostasis. The feeding center senses the systemic energy status by detecting hormone and nutrient levels for homeostatic regulation, resulting in the control of food intake, heat production, and glucose production and uptake. The concentration of glucose is sensed by two types of glucose-sensing neurons in the feeding center: glucose-excited neurons and glucose-inhibited neurons. Previous studies have mainly focused on glucose metabolism as the mechanism underlying glucose sensing. Recent studies have indicated that receptor-mediated pathways also play a role in glucose sensing. This review describes sweet taste receptors in the hypothalamus and explores the role of sweet taste receptors in energy homeostasis.

Immobilization and mineralization of N and P by heterotrophic microbes during leaf decomposition

Treesearch

Beth Cheever; Erika Kratzer; Jackson Webster

2012-01-01

According to theory, the rate and stoichiometry of microbial mineralization depend, in part, on nutrient availability. For microbes associated with leaves in streams, nutrients are available from both the water column and the leaf. Therefore, microbial nutrient cycling may change with nutrient availability and during leaf decomposition. We explored spatial and temporal...

The influence of subsurface porosity and bedrock composition on ecosystem productivity and drought resilience in the Sierra Nevada Batholith, California

NASA Astrophysics Data System (ADS)

Riebe, C. S.; Callahan, R. P.; Goulden, M.; Pasquet, S.; Flinchum, B. A.; Taylor, N. J.; Holbrook, W. S.

2017-12-01

The availability of water and nutrients in soil and weathered rock influences the distribution of Earth's terrestrial life and regulates ecosystem vulnerability to land use and climate change. We explored these relationships by combining geochemical and geophysical measurements at three mid-elevation sites in the Sierra Nevada, California. Forest cover correlates strongly with bedrock composition across the sites, implying strong lithologic control on the ecosystem. We evaluated two hypotheses about bedrock-ecosystem connections: 1) that bedrock composition influences vegetation by moderating plant-essential nutrient supply; and 2) that bedrock composition influences the degree of subsurface weathering, which influences vegetation by controlling subsurface water-storage capacity. To quantify subsurface water-holding capacity, we used seismic refraction surveys to infer gradients in P and S-wave velocity structure, which reveal variations in porosity when coupled together in a Hertz-Mindlin rock-physics model. We combined the geophysical data on porosity with bedrock bulk geochemistry measured in previous work to evaluate the influence of water-holding capacity and nutrient supply on ecosystem productivity, which we quantified using remote sensing. Our results show that more than 80% of the variance in ecosystem productivity can be explained by differences in bedrock phosphorus concentration and subsurface porosity, with phosphorus content being the dominant explanatory variable. This suggests that bedrock composition exerts a strong bottom-up control on ecosystem productivity through its influence on nutrient supply and weathering susceptibility, which in turn influences porosity. We show that vegetation vulnerability to drought stress and mortality can be explained in part by variations in subsurface water-holding capacity and rock-derived nutrient supply.

Structural basis for leucine sensing by the Sestrin2-mTORC1 pathway.

PubMed

Saxton, Robert A; Knockenhauer, Kevin E; Wolfson, Rachel L; Chantranupong, Lynne; Pacold, Michael E; Wang, Tim; Schwartz, Thomas U; Sabatini, David M

2016-01-01

Eukaryotic cells coordinate growth with the availability of nutrients through the mechanistic target of rapamycin complex 1 (mTORC1), a master growth regulator. Leucine is of particular importance and activates mTORC1 via the Rag guanosine triphosphatases and their regulators GATOR1 and GATOR2. Sestrin2 interacts with GATOR2 and is a leucine sensor. Here we present the 2.7 angstrom crystal structure of Sestrin2 in complex with leucine. Leucine binds through a single pocket that coordinates its charged functional groups and confers specificity for the hydrophobic side chain. A loop encloses leucine and forms a lid-latch mechanism required for binding. A structure-guided mutation in Sestrin2 that decreases its affinity for leucine leads to a concomitant increase in the leucine concentration required for mTORC1 activation in cells. These results provide a structural mechanism of amino acid sensing by the mTORC1 pathway. Copyright © 2016, American Association for the Advancement of Science.

Spatial variability assessment of soil nutrients in an intense agricultural area, a case study of Rugao County in Yangtze River Delta Region, China

NASA Astrophysics Data System (ADS)

Zhao, Yongcun; Xu, Xianghua; Darilek, Jeremy Landon; Huang, Biao; Sun, Weixia; Shi, Xuezheng

2009-05-01

Topsoil samples (0-20 cm) ( n = 237) were collected from Rugao County, China. Geostatistical variogram analysis, sequential Gaussian simulation (SGS), and principal component (PC) analysis were applied to assess spatial variability of soil nutrients, identify the possible areas of nutrient deficiency, and explore spatial scale of variability of soil nutrients in the county. High variability of soil nutrient such as soil organic matter (SOM), total nitrogen (TN), available P, K, Fe, Mn, Cu, Zn, and B concentrations were observed. Soil nutrient properties displayed significant differences in their spatial structures, with available Cu having strong spatial dependence, SOM and available P having weak spatial dependence, and other nutrient properties having moderate spatial dependence. The soil nutrient deficiency, defined here as measured nutrient concentrations which do not meet the advisory threshold values specific to the county for dominant crops, namely rice, wheat, and rape seeds, was observed in available K and Zn, and the deficient areas covered 38 and 11%, respectively. The first three PCs of the nine soil nutrient properties explained 62.40% of the total variance. TN and SOM with higher loadings on PC1 are closely related to soil texture derived from different parent materials. The PC2 combined intermediate response variables such as available Zn and P that are likely to be controlled by land use and soil pH. Available B has the highest loading on PC3 and its variability of concentrations may be primarily ascribed to localized anthropogenic influence. The amelioration of soil physical properties (i.e. soil texture) and soil pH may improve the availability of soil nutrients and the sustainability of the agricultural system of Rugao County.

Evaluating nitrogen removal by vegetation uptake using satellite image time series in riparian catchments.

PubMed

Wang, Xuelei; Wang, Qiao; Yang, Shengtian; Zheng, Donghai; Wu, Chuanqing; Mannaerts, C M

2011-06-01

Nitrogen (N) removal by vegetation uptake is one of the most important functions of riparian buffer zones in preventing non-point source pollution (NSP), and many studies about N uptake at the river reach scale have proven the effectiveness of plants in controlling nutrient pollution. However, at the watershed level, the riparian zones form dendritic networks and, as such, may be the predominant spatially structured feature in catchments and landscapes. Thus, assessing the functions of riparian system at the basin scale is important. In this study, a new method coupling remote sensing and ecological models was used to assess the N removal by riparian vegetation on a large spatial scale. The study site is located around the Guanting reservoir in Beijing, China, which was abandoned as the source water system for Beijing due to serious NSP in 1997. SPOT 5 data was used to map the land cover, and Landsat-5 TM time series images were used to retrieve land surface parameters. A modified forest nutrient cycling and biomass model (ForNBM) was used to simulate N removal, and the modified net primary productivity (NPP) module was driven by remote sensing image time series. Besides the remote sensing data, the necessary database included meteorological data, soil chemical and physical data and plant nutrient data. Pot and plot experiments were used to calibrate and validate the simulations. Our study has proven that, by coupling remote sensing data and parameters retrieval techniques to plant growth process models, catchment scale estimations of nitrogen uptake rates can be improved by spatial pixel-based modelling. Copyright © 2011 Elsevier B.V. All rights reserved.

Great Lakes Research Review, 1982. Appendices.

DTIC Science & Technology

1982-11-01

Annul Report - societal impact of the Cladophora problem (p. 6) - long-term, large-scale drift and dispersal pattern; ii the Greit akes (p. 7) C...Nutrient Exchange Processes in Macrophyte Lakes 155 C 479 NWRI Cladophora in the Great Lakes 156 C 494 NWRI Lake Ontario Nutrient Assessment Study (LONAS...Ingested with Drinking Water 136 A 387 UM Cladophora Measurements using Remote Sensing 137 A 387 U.WI. Nutrition of Great Lakes Cladophora 138 A 393

Epiphyte loads on seagrasses and microphytobenthos abundance are not reliable indicators of nutrient availability in oligotrophic coastal ecosystems.

PubMed

Fourqurean, James W; Muth, Meredith F; Boyer, Joseph N

2010-07-01

Despite marked gradients in nutrient availability that control the abundance and species composition of seagrasses in south Florida, and the importance of nutrient availability in controlling abundance and composition of epiphytes on seagrasses in other locations, we did not find that epiphyte load on the dominant seagrass, Thalassia testudinum, or that the relative contribution of algal epiphytes to the epiphyte community, was positively correlated with nutrient availability in the water column or the sediment in oligotrophic seagrass beds. Further, the abundance of microphytobenthos, as indicated by Chlorophyll-a concentration in the sediments, was not directly correlated with concentrations of nutrients in the sediments. Our results suggest that epiphyte and microphytobenthos abundance are not unambiguous indicators of nutrient availability in relatively pristine seagrass environments, and therefore would make poor candidates for indicators of the status and trends of seagrass ecosystems in relatively low-nutrient environments like the Florida Keys. Copyright 2010 Elsevier Ltd. All rights reserved.

Plants may alter competition by modifying nutrient bioavailability in rhizosphere: a modeling approach.

PubMed

Raynaud, Xavier; Jaillard, Benoît; Leadley, Paul W

2008-01-01

Plants modify nutrient availability by releasing chemicals in the rhizosphere. This change in availability induced by roots (bioavailability) is known to improve nutrient uptake by individual plants releasing such compounds. Can this bioavailability alter plant competition for nutrients and under what conditions? To address these questions, we have developed a model of nutrient competition between plant species based on mechanistic descriptions of nutrient diffusion, plant exudation, and plant uptake. The model was parameterized using data of the effects of root citrate exudation on phosphorus availability. We performed a sensitivity analysis for key parameters to test the generality of these effects. Our simulations suggest the following. (1) Nutrient uptake depends on the number of roots when nutrients and exudates diffuse little, because individual roots are nearly independent in terms of nutrient supply. In this case, bioavailability profits only species with exudates. (2) Competition for nutrients depends on the spatial arrangement of roots when nutrients diffuse little but exudates diffuse widely. (3) Competition for nutrients depends on the nutrient uptake capacity of roots when nutrients and exudates diffuse widely. In this case, bioavailability profits all species. Mechanisms controlling competition for bioavailable nutrients appear to be diverse and strongly depend on soil, nutrient, and plant properties.

Octopamine connects nutrient cues to lipid metabolism upon nutrient deprivation.

PubMed

Tao, Jun; Ma, Yi-Cheng; Yang, Zhong-Shan; Zou, Cheng-Gang; Zhang, Ke-Qin

2016-05-01

Starvation is probably the most common stressful situation in nature. In vertebrates, elevation of the biogenic amine norepinephrine levels is common during starvation. However, the precise role of norepinephrine in nutrient deprivation remains largely unknown. We report that in the free-living nematode Caenorhabditis elegans, up-regulation of the biosynthesis of octopamine, the invertebrate counterpart of norepinephrine, serves as a mechanism to adapt to starvation. During nutrient deprivation, the nuclear receptor DAF-12, known to sense nutritional cues, up-regulates the expression of tbh-1 that encodes tyramine β-hydroxylase, a key enzyme for octopamine biosynthesis, in the RIC neurons. Octopamine induces the expression of the lipase gene lips-6 via its receptor SER-3 in the intestine. LIPS-6, in turn, elicits lipid mobilization. Our findings reveal that octopamine acts as an endocrine regulator linking nutrient cues to lipolysis to maintain energy homeostasis, and suggest that such a mechanism may be evolutionally conserved in diverse organisms.

Plant–herbivore–decomposer stoichiometric mismatches and nutrient cycling in ecosystems

PubMed Central

Cherif, Mehdi; Loreau, Michel

2013-01-01

Plant stoichiometry is thought to have a major influence on how herbivores affect nutrient availability in ecosystems. Most conceptual models predict that plants with high nutrient contents increase nutrient excretion by herbivores, in turn raising nutrient availability. To test this hypothesis, we built a stoichiometrically explicit model that includes a simple but thorough description of the processes of herbivory and decomposition. Our results challenge traditional views of herbivore impacts on nutrient availability in many ways. They show that the relationship between plant nutrient content and the impact of herbivores predicted by conceptual models holds only at high plant nutrient contents. At low plant nutrient contents, the impact of herbivores is mediated by the mineralization/immobilization of nutrients by decomposers and by the type of resource limiting the growth of decomposers. Both parameters are functions of the mismatch between plant and decomposer stoichiometries. Our work provides new predictions about the impacts of herbivores on ecosystem fertility that depend on critical interactions between plant, herbivore and decomposer stoichiometries in ecosystems. PMID:23303537

The effect of pH on phosphorus availability and speciation in an aquaponics nutrient solution.

PubMed

Cerozi, Brunno da Silva; Fitzsimmons, Kevin

2016-11-01

The interaction between the main ions in aquaponics nutrient solutions affects chemical composition and availability of nutrients, and nutrient uptake by plant roots. This study determined the effect of pH on phosphorus (P) speciation and availability in an aquaponics nutrient solution and used Visual MINTEQ to simulate P species and P activity. In both experimental and simulated results, P availability decreased with increase in pH of aquaponics nutrient solutions. According to simulations, P binds to several cations leaving less free phosphate ions available in solution. High pH values resulted in the formation of insoluble calcium phosphate species. The study also demonstrated the importance of organic matter and alkalinity in keeping free phosphate ions in solution at high pH ranges. It is recommended though that pH in aquaponics systems is maintained at a 5.5-7.2 range for optimal availability and uptake by plants. Copyright © 2016 Elsevier Ltd. All rights reserved.

Bacillus Probiotic Enzymes: External Auxiliary Apparatus to Avoid Digestive Deficiencies, Water Pollution, Diseases, and Economic Problems in Marine Cultivated Animals.

PubMed

Olmos Soto, Jorge

Exploitation of marine fishes is the main source of several life-supporting feed compounds such as proteins, lipids, and carbohydrates that maintain the production of most trading marine organisms by aquaculture. However, at this rate the marine inventory will go to the end soon, since fishery resources are finite. In this sense, the availability of the principal ingredients obtained from marine fishes is going to decrease considerably, increasing the diet prices and affecting the economy of this activity. Therefore, aquaculture industry needs to find nonexpensive land unconventional resources of protein, carbohydrates, and lipids and use bacterial probiotics to improve digestion-assimilation of these unfamiliar compounds. Bacillus subtilis is a cosmopolitan probiotic bacterium with a great enzymatic profile that could improve nutrient digestion-assimilation, induce healthy growth, and avoid water pollution, decreasing economic problems and increasing yields in the aquaculture industry. In this chapter, we present how Bacillus enzymes can help marine animals to assimilate nutrients from unconventional and economic plant resources. © 2017 Elsevier Inc. All rights reserved.

Sagebrush wildfire effects on surface soil nutrient availability: A temporal and spatial study

USDA-ARS?s Scientific Manuscript database

Wildfires occurring in Artemisia (sagebrush) ecosystems can temporarily increase soil nutrient availability in surface soil. Less is known, however, on how soil nutrient availability changes over time and microsite location post-wildfire. In Oct., 2013 a wildfire approximately 30 km north of Reno, N...

Dynamics of the transition zone in coastal zone color scanner-sensed ocean color in the North Pacific during oceanographic spring

NASA Technical Reports Server (NTRS)

Glover, David M.; Wroblewski, J. S.; Mcclain, Charles R.

1994-01-01

A transition zone in phytoplankton concentration running across the North Pacific basin at 30 deg to 40 deg north latitude corresponds to a basin-wide front in surface chlorophyll observed in a composite of coastal zone color scanner (CZCS) images for May, June, and July 1979-1986. This transition zone with low chlorophyll to the south and higher chlorophyll to the north can be simulated by a simple model of the concentration of phytoplankton, zooplankton, and dissolved nutrient (nitrate) in the surface mixed layer of the ocean applied to the North Pacific basin for the climatological conditions during oceanographic springtime (May, June, and July). The model is initialized with a 1 deg x 1 deg gridded estimate of wintertime (February, March, and April) mixed layer nitrate concentrations calculated from an extensive nutrient database and a similarly gridded mixed layer depth data set. Comparison of model predictions with CZCS data provides a means of evaluating the dynamics of the transition zone. We conclude that in the North Pacific, away from major boundary currents and coastal upwelling zones, wintertime vertical mixing determines the total nutrient available to the plankton ecosystem in the spring. The transition zone seen in basin-scale CZCS images is a reflection of the geographic variation in the wintertime mixed layer depth and the nitracline, leading to a latitudinal gradient in phytoplankton chlorophyll.

Rainfall runoff modelling of the Upper Ganga and Brahmaputra basins using PERSiST.

PubMed

Futter, M N; Whitehead, P G; Sarkar, S; Rodda, H; Crossman, J

2015-06-01

There are ongoing discussions about the appropriate level of complexity and sources of uncertainty in rainfall runoff models. Simulations for operational hydrology, flood forecasting or nutrient transport all warrant different levels of complexity in the modelling approach. More complex model structures are appropriate for simulations of land-cover dependent nutrient transport while more parsimonious model structures may be adequate for runoff simulation. The appropriate level of complexity is also dependent on data availability. Here, we use PERSiST; a simple, semi-distributed dynamic rainfall-runoff modelling toolkit to simulate flows in the Upper Ganges and Brahmaputra rivers. We present two sets of simulations driven by single time series of daily precipitation and temperature using simple (A) and complex (B) model structures based on uniform and hydrochemically relevant land covers respectively. Models were compared based on ensembles of Bayesian Information Criterion (BIC) statistics. Equifinality was observed for parameters but not for model structures. Model performance was better for the more complex (B) structural representations than for parsimonious model structures. The results show that structural uncertainty is more important than parameter uncertainty. The ensembles of BIC statistics suggested that neither structural representation was preferable in a statistical sense. Simulations presented here confirm that relatively simple models with limited data requirements can be used to credibly simulate flows and water balance components needed for nutrient flux modelling in large, data-poor basins.

Physical Control of Biological Productivity Off the Coast of Peru During the 1997-1998 El Nino

NASA Technical Reports Server (NTRS)

Carr, Mary-Elena

1999-01-01

Satellite observations and an ecosystem model are used to understand the variability in the planktonic ecosystem off Peru for the period January 1996 to May 1998. The objective of this study is to quantify the changes in the ecosystem components, carbon pathways, and available food for small pelagic fish that occur associated with the change in physical forcing due to El Nino. Two periods are distinguished based on the observed sea level anomaly: a La Nina (LaN) period (1996) in which sea level was below normal and El Nino (EN), the average conditions for December 1997, in which the sea level was anomalously high. There are three phytoplankton size classes (pico-, nano-, and net-phytoplankton) which compete for nutrients and are eaten by three zooplankton size classes. The ecosystem model is forced by alongshore wind speed measured by the NASA Scatterometer (NSCAT) and the European Remote-sensing Satellites (ERS-1 and ERS-2). Larger, slower growing organisms are more sensitive to physical disturbance than smaller organisms (Carr, 1998]. In the present simulation as well, the primary effect of the El Nino (reduced nutrient supply, and increased temperature) is to reduce the biomass of large cells (netphytoplankton) and consequently of the zooplankton that rely on large cells as food source. EN conditions are accompanied by a rearrangement of carbon pathways: comparable uptake goes into reduced biomass accumulation, increased losses to respiration, reduced carbon export, and much reduced carbon available to fish. The star indicates the remotely sensed biomass (assuming a constant carbon to chlorophyll ratio of 60) as measured by the Ocean Color and Temperature Sensor (Nov.-Dec. 1996) and the Sea-viewing Wide Field-of-view Sensor (Dec. 1997). The model, which assumes no light limitation, overestimates total phytoplankton biomass. Additional Information is contained in the original.

Real-time x-ray fluorescence analysis of crop canopy to spatially assess phytoextraction efficiency and subsurface status of low-Z elements: a case study for phosphorus

NASA Astrophysics Data System (ADS)

Dao, Thanh

2017-04-01

Leaf analysis has been extensively used to interpret results of nutrient supplementation studies about crop growth and yield responses, and to define availability thresholds for a wide range of soils and climatic conditions. The compositional results reflect the nutritional status, uptake efficiency, and the geo-chemical environment of the element in the subsurface. An X-ray fluorescence (XRF)-based proximal sensing approach was evaluated and proposed for real-time determination of water content and element-specific composition of corn seedling leaves, which was comprised mostly of essential macronutrients of low-atomic number Z, such as phosphorus (P) or potassium. Intensities of scattered radiation associated with the X-ray tube Ag anode were significantly correlated with leaf water content (θw), which was used to normalize fluorescence intensities of P. Crop canopy water status was also obtained as ancillary data. The θw - P relative concentration relationship was best described by a sigmoidal function (r2 = 0.938 and RMSE=0.02). The Ag-Lα line was deemed to be effective for normalizing the intensities of Kα lines of P and other low-Z elements, in addition to the commonly used Kα and Kβ lines. Its intensity was significantly correlated to leaf water content and was used to develop calibrations and obtain P concentration on a dry weight basis and unbiased estimates of crop P status. Therefore, the in situ fluorescence sensing system presents a new paradigm in nutrient management to re-evaluate calibrations of observed crop responses against those predicted by current soil testing and fertility recommendations. Updates to the rates of supplemental P and crop growth response relationships are critically needed as crop cultivars, supplemental P sources, or alternative soil-crop management systems are continually changing. Changes in soil microenvironments that are site- or field-specific, and climate are expected to continue to be the norm and can modify those soil-plant relationships. The high-throughput of hand-held XRFS enhances our ability to make management adjustment, particularly at the short early stages of growth, when crop plants are most susceptible to P deficiency. The precision of macronutrient management can be applied at a field-specific scale. As the process can be repeated for each growing season, the knowledge base of soil fertility, crop extraction efficiency and uptake, and elemental availability can only grow in time to improve the predictability of site-specific plant responses to given yield goals and levels of nutrient and soil management inputs. Matching nutrient supply to actual levels needed by the crop minimizes loss of excess agricultural inputs and reduces the risks of adverse impact on the health of the surrounding soil and water resources.

Risk evaluation of available phosphorus loss in agricultural land based on remote sensing and GIS

NASA Astrophysics Data System (ADS)

Ding, Xiaodong; Zhou, Bin; Xu, Junfeng; Liu, Ting; Xie, Bin

2010-09-01

The surplus of phosphorus leads to water eutrophication. Huge input of fertilizers in agricultural activities enriches nutrition in soil. The superfluous nutrient moves easily to riparian water by rainfall and surface runoff; leads to water eutrophication of riparian wetlands and downstream water; and consequently affects ecological balance. Thus it is significant to investigate the risk of phosphorus loss in agricultural land, to identify high concentration areas and guide the management of nutrition loss. This study was implemented mainly in the area of agricultural use in southern Western Australia, where a three-year period preliminary monitoring of water quality showed that the concentration of different forms of phosphorus in water had far exceeded the standard. Due to the large scale surface runoff caused by occasional storms in Western Australia, soil erosion was selected as the main driving factor for the loss of phosphorus. Remote sensing and ground truth data were used to reflect the seasonal changes of plants. The spatial distribution of available phosphorus was then predicted and combined with the evaluation matrix to evaluate the loss risk of phosphorus. This evaluation was based on quantitative rather than qualitative data to make better precision. It could help making decision support for monitoring water quality of rivers and riparian wetlands.

Photographic Remote Sensing of Sick Citrus Trees

NASA Technical Reports Server (NTRS)

Gausman, H. W.

1971-01-01

Remote sensing with infrared color aerial photography (Kodak Ektachrome Infrared Aero 8443 film) for detecting citrus tree anomalies is described. Illustrations and discussions are given for detecting nutrient toxicity symptoms, for detecting foot rot and sooty mold fungal diseases, and for distinguishing among citrus species. Also, the influence of internal leaf structure on light reflectance, transmittance, and absorptance are considered; and physiological and environmental factors that affect citrus leaf light reflectance are reviewed briefly and illustrated.

Ocean-color remote sensing of the Nile delta shelf and SE Levantine basin and possible linkage to some mesoscale circulation features and Nile river run-off

NASA Astrophysics Data System (ADS)

Moufaddal, Wahid; Lavender, Samantha

To date, and despite the passage of more than 30 years since the launch of the first satellite based ocean-color sensor, no systematic study of the variability of chlorophyll in the Egyptian Mediterranean coast off the Nile delta has been undertaken using this kind of data. Meantime, available in-situ measurements on chlorophyll and other nutrient parameters along this coast are indeed very modest and scarce. The lack of data has in turn created a large gap in our knowledge on the biogeochemical characteristics of the coastal water and impacts of the Aswan High Dam and other land-use changes on the marine ecosystems and nutrient budget in the Nile delta shelf and the SE Mediterranean. The present study aims to fill part of this gap through application of ocean-color remote sensing and satellite retrieval of phytoplankton chlorophyll. For this purpose a 10-year (1997-2006) monthly satellite dataset from ESA Globcolour project (an ESA Data User Element project: http://www.globcolour.info) was retrieved and subjected to time-series analysis. Results of this analysis revealed that the oceanic and coastal parts off the Nile delta coast and SE Mediterranean manifest from time to time some of the most interesting and dynamical marine features including meso-scale gyres, coastal filaments, localized algal blooms and higher concentration of phytoplankton chlorophyll. These features together with certain physical pro-cesses and surface run-off from Nile mouthes and other land-based sources were found to exert pronounced effects on the nutrient supply and quality of the coastal and oceanic surface waters in this region. Results reveled also that there has been a general upward trend in concentration of surface chlorophyll during the 10-year period from 1997 to 2006 with a coincident rise of the coastal fisheries implying that improvement of nutrient supply is most likely responsible for this rise. Results confirmed also shift of the Nile phytoplankton bloom in space and time after construc-tion of the Aswan High Dam and other subsequent anthropogenic activities Conclusions and results achieved by this study show the importance of ocean-color satellite data for monitoring of biogeochemical impacts of land-use changes on coastal ecosystems and the role which it can play in assessment of the marine productivity and state of the marine fisheries.

An approach to developing numeric water quality criteria for coastal waters using the SeaWiFS Satellite Data Record.

PubMed

Schaeffer, Blake A; Hagy, James D; Conmy, Robyn N; Lehrter, John C; Stumpf, Richard P

2012-01-17

Human activities on land increase nutrient loads to coastal waters, which can increase phytoplankton production and biomass and associated ecological impacts. Numeric nutrient water quality standards are needed to protect coastal waters from eutrophication impacts. The Environmental Protection Agency determined that numeric nutrient criteria were necessary to protect designated uses of Florida's waters. The objective of this study was to evaluate a reference condition approach for developing numeric water quality criteria for coastal waters, using data from Florida. Florida's coastal waters have not been monitored comprehensively via field sampling to support numeric criteria development. However, satellite remote sensing had the potential to provide adequate data. Spatial and temporal measures of SeaWiFS OC4 chlorophyll-a (Chl(RS)-a, mg m(-3)) were resolved across Florida's coastal waters between 1997 and 2010 and compared with in situ measurements. Statistical distributions of Chl(RS)-a were evaluated to determine a quantitative reference baseline. A binomial approach was implemented to consider how new data could be assessed against the criteria. The proposed satellite remote sensing approach to derive numeric criteria may be generally applicable to other coastal waters.

An Approach to Developing Numeric Water Quality Criteria for Coastal Waters Using the SeaWiFS Satellite Data Record

PubMed Central

2011-01-01

Human activities on land increase nutrient loads to coastal waters, which can increase phytoplankton production and biomass and associated ecological impacts. Numeric nutrient water quality standards are needed to protect coastal waters from eutrophication impacts. The Environmental Protection Agency determined that numeric nutrient criteria were necessary to protect designated uses of Florida’s waters. The objective of this study was to evaluate a reference condition approach for developing numeric water quality criteria for coastal waters, using data from Florida. Florida’s coastal waters have not been monitored comprehensively via field sampling to support numeric criteria development. However, satellite remote sensing had the potential to provide adequate data. Spatial and temporal measures of SeaWiFS OC4 chlorophyll-a (ChlRS-a, mg m–3) were resolved across Florida’s coastal waters between 1997 and 2010 and compared with in situ measurements. Statistical distributions of ChlRS-a were evaluated to determine a quantitative reference baseline. A binomial approach was implemented to consider how new data could be assessed against the criteria. The proposed satellite remote sensing approach to derive numeric criteria may be generally applicable to other coastal waters. PMID:22192062

Carnitine acetyltransferase (CRAT) expression in macrophages is dispensable for nutrient stress sensing and inflammation.

PubMed

Goldberg, Emily L; Dixit, Vishwa Deep

2017-02-01

Fatty acid oxidation in macrophages is thought to regulate inflammatory status and insulin-sensitivity. An important unanswered question in this field is whether carnitine acetyl-transferase (CrAT) that regulates fatty acid oxidation and mitochondrial acetyl-CoA balance is required to integrate nutrient stress sensing to inflammatory response in macrophages. Mice with myeloid lineage-specific Crat deletion were subjected to several metabolic stressors, including high-fat diet-induced obesity, fasting, and LPS-induced endotoxemia. Their metabolic homeostasis was compared to that of Crat-sufficient littermate controls. Inflammatory potential of Crat-deficient and Crat-sufficient macrophages were measured both in vitro and in vivo . Our studies revealed that ablation of CrAT in myeloid lineage cells did not impact glucose homeostasis, insulin-action, adipose tissue leukocytosis, and inflammation when animals were confronted with a variety of metabolic stressors, including high-fat diet, fasting, or LPS-induced acute endotoxemia. These findings demonstrate that unlike muscle cells, substrate switch mechanisms that control macrophage energy metabolism and mitochondrial short-chain acyl-CoA pools during nutrient stress are controlled by pathways that are not solely reliant on CrAT.

Spatial Differentiation of Arable Land and Permanent Grasslands to Improve a Regional Land Management Model for Nutrient Balancing

NASA Astrophysics Data System (ADS)

Gómez Giménez, M.; Della Peruta, R.; de Jong, R.; Keller, A.; Schaepman, M. E.

2015-12-01

Agroecosystems play an important role providing economic and ecosystem services, which directly impact society. Inappropriate land use and unsustainable agricultural management with associated nutrient cycles can jeopardize important soil functions such as food production, livestock feeding and conservation of biodiversity. The objective of this study was to integrate remotely sensed land cover information into a regional Land Management Model (LMM) to improve the assessment of spatial explicit nutrient balances for agroecosystems. Remotely sensed data as well as an optimized parameter set contributed to feed the LMM providing a better spatial allocation of agricultural data aggregated at farm level. The integration of land use information in the land allocation process relied predominantly on three factors: i) spatial resolution, ii) classification accuracy and iii) parcels definition. The best-input parameter combination resulted in two different land cover classifications with overall accuracies of 98%, improving the LMM performance by 16% as compared to using non-spatially explicit input. Firstly, the use of spatial explicit information improved the spatial allocation output resulting in a pattern that better followed parcel boundaries (Figure 1). Second, the high classification accuracies ensured consistency between the datasets used. Third, the use of a suitable spatial unit to define the parcels boundaries influenced the model in terms of computational time and the amount of farmland allocated. We conclude that the combined use of remote sensing (RS) data with the LMM has the potential to provide highly accurate information of spatial explicit nutrient balances that are crucial for policy options concerning sustainable management of agricultural soils. Figure 1. Details of the spatial pattern obtained: a) Using only the farm census data, b) using also land use information. Framed in black in the left image (a), examples of artifacts that disappeared when using land use information (right image, b). Colors represent different ownership.

Context-dependent effects of nutrient loading on the coral-algal mutualism.

PubMed

Shantz, Andrew A; Burkepile, Deron E

2014-07-01

Human-mediated increases in nutrient availability alter patterns of primary production, impact species diversity, and threaten ecosystem function. Nutrients can also alter community structure by disrupting the relationships between nutrient-sharing mutualists that form the foundation of communities. Given their oligotrophic nature and the dependence of reef-building corals on symbiotic relationships, coral reefs may be particularly vulnerable to excess nutrients. However, individual studies suggest complex, even contradictory, relationships among nutrient availability, coral physiology, and coral growth. Here, we used meta-analysis to establish general patterns of the impact of nitrogen (N) and phosphorus (P) on coral growth and photobiology. Overall, we found that over a wide range of concentrations, N reduced coral calcification 11%, on average, but enhanced metrics of coral photobiology, such as photosynthetic rate. In contrast, P enrichment increased average calcification rates by 9%, likely through direct impacts on the calcification process, but minimally impacted coral photobiology. There were few synergistic impacts of combined N and P on corals, as the nutrients impact corals via different pathways. Additionally, the response of corals to increasing nutrient availability was context dependent, varying with coral taxa and morphology, enrichment source, and nutrient identity. For example, naturally occurring enrichment from fish excretion increased coral growth, while human-mediated enrichment tended to decrease coral growth. Understanding the nuances of the relationship between nutrients and corals may allow for more targeted remediation strategies and suggest how other global change drivers such as overfishing and climate change will shape how nutrient availability impacts corals.

Response of plant nutrient stoichiometry to fertilization varied with plant tissues in a tropical forest

PubMed Central

Mo, Qifeng; Zou, Bi; Li, Yingwen; Chen, Yao; Zhang, Weixin; Mao, Rong; Ding, Yongzhen; Wang, Jun; Lu, Xiankai; Li, Xiaobo; Tang, Jianwu; Li, Zhian; Wang, Faming

2015-01-01

Plant N:P ratios are widely used as indices of nutrient limitation in terrestrial ecosystems, but the response of these metrics in different plant tissues to altered N and P availability and their interactions remains largely unclear. We evaluated changes in N and P concentrations, N:P ratios of new leaves (<1 yr), older leaves (>1 yr), stems and mixed fine roots of seven species after 3-years of an N and P addition experiment in a tropical forest. Nitrogen addition only increased fine root N concentrations. P addition increased P concentrations among all tissues. The N × P interaction reduced leaf and stem P concentrations, suggesting a negative effect of N addition on P concentrations under P addition. The reliability of using nutrient ratios as indices of soil nutrient availability varied with tissues: the stoichiometric metrics of stems and older leaves were more responsive indicators of changed soil nutrient availability than those of new leaves and fine roots. However, leaf N:P ratios can be a useful indicator of inter-specific variation in plant response to nutrients availability. This study suggests that older leaf is a better choice than other tissues in the assessment of soil nutrient status and predicting plant response to altered nutrients using nutrients ratios. PMID:26416169

Soil Macronutrient Sensing for Precision Agriculture

USDA-ARS?s Scientific Manuscript database

Accurate measurements of soil macronutrients (i.e., nitrogen, phosphorus, and potassium) are needed for efficient agricultural production, including site-specific crop management (SSCM), where fertilizer nutrient application rates are adjusted spatially based on local requirements. Rapid, non-destru...

Physiological links of circadian clock and biological clock of aging.

PubMed

Liu, Fang; Chang, Hung-Chun

2017-07-01

Circadian rhythms orchestrate biochemical and physiological processes in living organisms to respond the day/night cycle. In mammals, nearly all cells hold self-sustained circadian clocks meanwhile couple the intrinsic rhythms to systemic changes in a hierarchical manner. The suprachiasmatic nucleus (SCN) of the hypothalamus functions as the master pacemaker to initiate daily synchronization according to the photoperiod, in turn determines the phase of peripheral cellular clocks through a variety of signaling relays, including endocrine rhythms and metabolic cycles. With aging, circadian desynchrony occurs at the expense of peripheral metabolic pathologies and central neurodegenerative disorders with sleep symptoms, and genetic ablation of circadian genes in model organisms resembled the aging-related features. Notably, a number of studies have linked longevity nutrient sensing pathways in modulating circadian clocks. Therapeutic strategies that bridge the nutrient sensing pathways and circadian clock might be rational designs to defy aging.

Effects of harvesting on nitrogen and phosphorus availability in riparian management zone soils in Minnesota, USA

Treesearch

Douglas N. Kastendick; Eric K. Zenner; Brian J. Palik; Randall K. Kolka; Charles R. Blinn

2012-01-01

Riparian management zones (RMZs) protect streams from excess nutrients, yet few studies have looked at soil nutrients in forested RMZs or the impacts of partial harvesting on nutrient availability. We investigated the impacts of upland clearcutting in conjunction with uncut and partially harvested RMZs (40% basal area reduction) on soil nutrients in forests in...

Nutrients for the aging eye

PubMed Central

Rasmussen, Helen M; Johnson, Elizabeth J

2013-01-01

The incidence of age-related eye diseases is expected to rise with the aging of the population. Oxidation and inflammation are implicated in the etiology of these diseases. There is evidence that dietary antioxidants and anti-inflammatories may provide benefit in decreasing the risk of age-related eye disease. Nutrients of interest are vitamins C and E, β-carotene, zinc, lutein, zeaxanthin, and the omega-3 fatty acids eicosapentaenoic acid and docosahexaenoic acid. While a recent survey finds that among the baby boomers (45–65 years old), vision is the most important of the five senses, well over half of those surveyed were not aware of the important nutrients that play a key role in eye health. This is evident from a national survey that finds that intake of these key nutrients from dietary sources is below the recommendations or guidelines. Therefore, it is important to educate this population and to create an awareness of the nutrients and foods of particular interest in the prevention of age-related eye disease. PMID:23818772

Octopamine connects nutrient cues to lipid metabolism upon nutrient deprivation

PubMed Central

Tao, Jun; Ma, Yi-Cheng; Yang, Zhong-Shan; Zou, Cheng-Gang; Zhang, Ke-Qin

2016-01-01

Starvation is probably the most common stressful situation in nature. In vertebrates, elevation of the biogenic amine norepinephrine levels is common during starvation. However, the precise role of norepinephrine in nutrient deprivation remains largely unknown. We report that in the free-living nematode Caenorhabditis elegans, up-regulation of the biosynthesis of octopamine, the invertebrate counterpart of norepinephrine, serves as a mechanism to adapt to starvation. During nutrient deprivation, the nuclear receptor DAF-12, known to sense nutritional cues, up-regulates the expression of tbh-1 that encodes tyramine β-hydroxylase, a key enzyme for octopamine biosynthesis, in the RIC neurons. Octopamine induces the expression of the lipase gene lips-6 via its receptor SER-3 in the intestine. LIPS-6, in turn, elicits lipid mobilization. Our findings reveal that octopamine acts as an endocrine regulator linking nutrient cues to lipolysis to maintain energy homeostasis, and suggest that such a mechanism may be evolutionally conserved in diverse organisms. PMID:27386520

Nutrition acquisition strategies during fungal infection of plants.

PubMed

Divon, Hege H; Fluhr, Robert

2007-01-01

In host-pathogen interactions, efficient pathogen nutrition is a prerequisite for successful colonization and fungal fitness. Filamentous fungi have a remarkable capability to adapt and exploit the external nutrient environment. For phytopathogenic fungi, this asset has developed within the context of host physiology and metabolism. The understanding of nutrient acquisition and pathogen primary metabolism is of great importance in the development of novel disease control strategies. In this review, we discuss the current knowledge on how plant nutrient supplies are utilized by phytopathogenic fungi, and how these activities are controlled. The generation and use of auxotrophic mutants have been elemental to the determination of essential and nonessential nutrient compounds from the plant. Considerable evidence indicates that pathogen entrainment of host metabolism is a widespread phenomenon and can be accomplished by rerouting of the plant's responses. Crucial fungal signalling components for nutrient-sensing pathways as well as their developmental dependency have now been identified, and were shown to operate in a coordinate cross-talk fashion that ensures proper nutrition-related behaviour during the infection process.

Internal states drive nutrient homeostasis by modulating exploration-exploitation trade-off

PubMed Central

Corrales-Carvajal, Verónica María; Faisal, Aldo A; Ribeiro, Carlos

2016-01-01

Internal states can profoundly alter the behavior of animals. A quantitative understanding of the behavioral changes upon metabolic challenges is key to a mechanistic dissection of how animals maintain nutritional homeostasis. We used an automated video tracking setup to characterize how amino acid and reproductive states interact to shape exploitation and exploration decisions taken by adult Drosophila melanogaster. We find that these two states have specific effects on the decisions to stop at and leave proteinaceous food patches. Furthermore, the internal nutrient state defines the exploration-exploitation trade-off: nutrient-deprived flies focus on specific patches while satiated flies explore more globally. Finally, we show that olfaction mediates the efficient recognition of yeast as an appropriate protein source in mated females and that octopamine is specifically required to mediate homeostatic postmating responses without affecting internal nutrient sensing. Internal states therefore modulate specific aspects of exploitation and exploration to change nutrient selection. DOI: http://dx.doi.org/10.7554/eLife.19920.001 PMID:27770569

Small-scale drivers: the importance of nutrient availability and snowmelt timing on performance of the alpine shrub Salix herbacea.

PubMed

Little, Chelsea J; Wheeler, Julia A; Sedlacek, Janosch; Cortés, Andrés J; Rixen, Christian

2016-04-01

Alpine plant communities are predicted to face range shifts and possibly extinctions with climate change. Fine-scale environmental variation such as nutrient availability or snowmelt timing may contribute to the ability of plant species to persist locally; however, variation in nutrient availability in alpine landscapes is largely unmeasured. On three mountains around Davos, Switzerland, we deployed Plant Root Simulator probes around 58 Salix herbacea plants along an elevational and microhabitat gradient to measure nutrient availability during the first 5 weeks of the summer growing season, and used in situ temperature loggers and observational data to determine date of spring snowmelt. We also visited the plants weekly to assess performance, as measured by stem number, fruiting, and herbivory damage. We found a wide snowmelt gradient which determined growing season length, as well as variations of an order of magnitude or more in the accumulation of 12 nutrients between different microhabitats. Higher nutrient availability had negative effects on most shrub performance metrics, for instance decreasing stem number and the proportion of stems producing fruits. High nutrient availability was associated with increased herbivory damage in early-melting microhabitats, but among late-emerging plants this pattern was reversed. We demonstrate that nutrient availability is highly variable in alpine settings, and that it strongly influences performance in an alpine dwarf shrub, sometimes modifying the response of shrubs to snowmelt timing. As the climate warms and human-induced nitrogen deposition continues in the Alps, these factors may contribute to patterns of local plants persistence.

Available nutrients in biochar

USDA-ARS?s Scientific Manuscript database

Biochar technology may contribute to the recovery and recycling of plant nutrients and thus add a fertilizer value to the biochar. Total nutrient content in biochars varies greatly and is mainly dependent on feedstock elemental composition and to a lesser extent on pyrolysis conditions. Availability...

Developing a Dynamic SPARROW Water Quality Decision Support System Using NASA Remotely-Sensed Products

NASA Astrophysics Data System (ADS)

Al-Hamdan, M. Z.; Smith, R. A.; Hoos, A.; Schwarz, G. E.; Alexander, R. B.; Crosson, W. L.; Srikishen, J.; Estes, M., Jr.; Cruise, J.; Al-Hamdan, A.; Ellenburg, W. L., II; Flores, A.; Sanford, W. E.; Zell, W.; Reitz, M.; Miller, M. P.; Journey, C. A.; Befus, K. M.; Swann, R.; Herder, T.; Sherwood, E.; Leverone, J.; Shelton, M.; Smith, E. T.; Anastasiou, C. J.; Seachrist, J.; Hughes, A.; Graves, D.

2017-12-01

The USGS Spatially Referenced Regression on Watershed Attributes (SPARROW) surface water quality modeling system has been widely used for long term, steady state water quality analysis. However, users have increasingly requested a dynamic version of SPARROW that can provide seasonal estimates of nutrients and suspended sediment to receiving waters. The goal of this NASA-funded project is to develop a dynamic decision support system to enhance the southeast SPARROW water quality model and finer-scale dynamic models for selected coastal watersheds through the use of remotely-sensed data and other NASA Land Information System (LIS) products. The spatial and temporal scale of satellite remote sensing products and LIS modeling data make these sources ideal for the purposes of development and operation of the dynamic SPARROW model. Remote sensing products including MODIS vegetation indices, SMAP surface soil moisture, and OMI atmospheric chemistry along with LIS-derived evapotranspiration (ET) and soil temperature and moisture products will be included in model development and operation. MODIS data will also be used to map annual land cover/land use in the study areas and in conjunction with Landsat and Sentinel to identify disturbed areas that might be sources of sediment and increased phosphorus loading through exposure of the bare soil. These data and others constitute the independent variables in a regression analysis whose dependent variables are the water quality constituents total nitrogen, total phosphorus, and suspended sediment. Remotely-sensed variables such as vegetation indices and ET can be proxies for nutrient uptake by vegetation; MODIS Leaf Area Index can indicate sources of phosphorus from vegetation; soil moisture and temperature are known to control rates of denitrification; and bare soil areas serve as sources of enhanced nutrient and sediment production. The enhanced SPARROW dynamic models will provide improved tools for end users to manage water quality in near real time and for the formulation of future scenarios to inform strategic planning. Time-varying SPARROW outputs will aid water managers in decision making regarding allocation of resources in protecting aquatic habitats, planning for harmful algal blooms, and restoration of degraded habitats, stream segments, or lakes.

Role of gut nutrient sensing in stimulating appetite and conditioning food preferences

PubMed Central

Ackroff, Karen

2012-01-01

The discovery of taste and nutrient receptors (chemosensors) in the gut has led to intensive research on their functions. Whereas oral sugar, fat, and umami taste receptors stimulate nutrient appetite, these and other chemosensors in the gut have been linked to digestive, metabolic, and satiating effects that influence nutrient utilization and inhibit appetite. Gut chemosensors may have an additional function as well: to provide positive feedback signals that condition food preferences and stimulate appetite. The postoral stimulatory actions of nutrients are documented by flavor preference conditioning and appetite stimulation produced by gastric and intestinal infusions of carbohydrate, fat, and protein. Recent findings suggest an upper intestinal site of action, although postabsorptive nutrient actions may contribute to flavor preference learning. The gut chemosensors that generate nutrient conditioning signals remain to be identified; some have been excluded, including sweet (T1R3) and fatty acid (CD36) sensors. The gut-brain signaling pathways (neural, hormonal) are incompletely understood, although vagal afferents are implicated in glutamate conditioning but not carbohydrate or fat conditioning. Brain dopamine reward systems are involved in postoral carbohydrate and fat conditioning but less is known about the reward systems mediating protein/glutamate conditioning. Continued research on the postoral stimulatory actions of nutrients may enhance our understanding of human food preference learning. PMID:22442194

Changes in hyperspectral reflectance signatures of lettuce leaves in response to macronutrient deficiencies

NASA Astrophysics Data System (ADS)

Pacumbaba, R. O.; Beyl, C. A.

2011-07-01

The adaptation of specific remote sensing and hyperspectral analysis techniques for the determination of incipient nutrient stress in plants could allow early detection and precision supplementation for remediation, important considerations for minimizing mass of advanced life support systems on space station and long term missions. This experiment was conducted to determine if hyperspectral reflectance could be used to detect nutrient stress in Lactuca sativa L. cv. Black Seeded Simpson. Lettuce seedlings were grown for 90 days in a greenhouse or growth chamber in vermiculite containing modified Hoagland's nutrient solution with key macronutrient elements removed in order to induce a range of nutrient stresses, including nitrogen, phosphorus, potassium, calcium, and magnesium. Leaf tissue nutrient concentrations were compared with corresponding spectral reflectances taken at the end of 90 days. Spectral reflectances varied with growing location, position on the leaf, and nutrient deficiency treatment. Spectral responses of lettuce leaves under macronutrient deficiency conditions showed an increase in reflectance in the red, near red, and infrared wavelength ranges. The data obtained suggest that spectral reflectance shows the potential as a diagnostic tool in predicting nutrient deficiencies in general. Overlapping of spectral signatures makes the use of wavelengths of narrow bandwidths or individual bands for the discrimination of specific nutrient stresses difficult without further data processing.

Invited review: nutrient-sensing receptors for free fatty acids and hydroxycarboxylic acids in farm animals.

PubMed

Mielenz, M

2017-06-01

Data on nutrient sensing by free fatty acid receptors (FFAR1, FFAR2, FFAR3, FFAR4) and hydroxycarboxylic acid receptors (HCAR1, HCAR2) are increasing for human or rodent models. Both receptor families link intestinal fermentation by the microbiota and energy metabolism with cellular responses. Therefore, this finding provides a link that is independent of the only function of the fermentation products as energy substrates. For example, these reactions are associated with insulin secretion, regulation of lipolysis, adipose tissue differentiation and innate immune responses. In farm animals, the available data on both receptor families from the intestine and other tissues increase. However, currently, the data are primarily linked with the distribution of receptor messenger RNAs (mRNAs) and more rarely with proteins. Functional data on the importance of these receptors in farm animal species is not abundant and is often associated with the immune system. In certain farm animal species, the receptors were cloned and ligand binding was characterised. In chicken, only one FFAR2 was recently identified using genome analysis, which is contradictory to a study using an FFAR1 small interfering RNA. The chicken FFAR2 is composed of more than 20 paralogs. No data on HCAR1 or HCAR2 exist in this species. Currently, in pigs, most available data are on the mRNA distribution within intestine. However, no FFAR1 expression has been shown in this organ to date. In addition to FFAR2, an orthologue (FFAR2-like) with the highest abundance in intestine has been reported. The data on HCAR1 and HCAR2 in pigs is scarce. In ruminants, most of the currently available information on receptor distribution is linked to mRNA data and shows the expression, for example, in mammary gland and adipose tissue. However, some protein data on FFAR2 and FFAR1 protein has been reported and functional data availability is slowly increasing. The receptor mRNAs of HCAR1 and HCAR2 are expressed in bovine. The HCAR2 protein has been demonstrated in certain tissues, such as liver and fat. Because of the physiological importance of both receptor families in human life science, more studies that analyse the physiological significance of both receptor families in animal science may be performed within the next several years.

Bioactive Molecules in Soil Ecosystems: Masters of the Underground

PubMed Central

Zhuang, Xuliang; Gao, Jie; Ma, Anzhou; Fu, Shenglei; Zhuang, Guoqiang

2013-01-01

Complex biological and ecological processes occur in the rhizosphere through ecosystem-level interactions between roots, microorganisms and soil fauna. Over the past decade, studies of the rhizosphere have revealed that when roots, microorganisms and soil fauna physically contact one another, bioactive molecular exchanges often mediate these interactions as intercellular signal, which prepare the partners for successful interactions. Despite the importance of bioactive molecules in sustainable agriculture, little is known of their numerous functions, and improving plant health and productivity by altering ecological processes remains difficult. In this review, we describe the major bioactive molecules present in below-ground ecosystems (i.e., flavonoids, exopolysaccharides, antibiotics and quorum-sensing signals), and we discuss how these molecules affect microbial communities, nutrient availability and plant defense responses. PMID:23615474

Teaching the basics of autophagy and mitophagy to redox biologists--mechanisms and experimental approaches.

PubMed

Zhang, Jianhua

2015-01-01

Autophagy is a lysosomal mediated degradation activity providing an essential mechanism for recycling cellular constituents, and clearance of excess or damaged lipids, proteins and organelles. Autophagy involves more than 30 proteins and is regulated by nutrient availability, and various stress sensing signaling pathways. This article provides an overview of the mechanisms and regulation of autophagy, its role in health and diseases, and methods for its measurement. Hopefully this teaching review together with the graphic illustrations will be helpful for instructors teaching graduate students who are interested in grasping the concepts and major research areas and introducing recent developments in the field. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

A novel nutrient sensing mechanism underlies substrate-induced regulation of monocarboxylate transporter-1

PubMed Central

Priyamvada, Shubha; Kumar, Anoop; Natarajan, Arivarasu A.; Gill, Ravinder K.; Alrefai, Waddah A.; Dudeja, Pradeep K.

2012-01-01

Monocarboxylate transporter isoform-1 (MCT1) plays an important role in the absorption of short-chain fatty acids (SCFAs) in the colon. Butyrate, a major SCFA, serves as the primary energy source for the colonic mucosa, maintains epithelial integrity, and ameliorates intestinal inflammation. Previous studies have shown substrate (butyrate)-induced upregulation of MCT1 expression and function via transcriptional mechanisms. The present studies provide evidence that short-term MCT1 regulation by substrates could be mediated via a novel nutrient sensing mechanism. Short-term regulation of MCT1 by butyrate was examined in vitro in human intestinal C2BBe1 and rat intestinal IEC-6 cells and ex vivo in rat intestinal mucosa. Effects of pectin feeding on MCT1, in vivo, were determined in rat model. Butyrate treatment (30–120 min) of C2BBe1 cells increased MCT1 function {p-(chloromercuri) benzene sulfonate (PCMBS)-sensitive [14C]butyrate uptake} in a pertussis toxin-sensitive manner. The effects were associated with decreased intracellular cAMP levels, increased Vmax of butyrate uptake, and GPR109A-dependent increase in apical membrane MCT1 level. Nicotinic acid, an agonist for the SCFA receptor GPR109A, also increased MCT1 function and decreased intracellular cAMP. Pectin feeding increased apical membrane MCT1 levels and nicotinate-induced transepithelial butyrate flux in rat colon. Our data provide strong evidence for substrate-induced enhancement of MCT1 surface expression and function via a novel nutrient sensing mechanism involving GPR109A as a SCFA sensor. PMID:22982338

Rhizosphere Environment and Labile Phosphorus Release from Organic Waste-Amended Soils.

NASA Astrophysics Data System (ADS)

Dao, Thanh H.

2015-04-01

Crop residues and biofertilizers are primary sources of nutrients for organic crop production. However, soils treated with large amounts of nutrient-enriched manure have elevated phosphorus (P) levels in regions of intensive animal agriculture. Surpluses occurred in these amended soils, resulting in large pools of exchangeable inorganic P (Pi) and enzyme-labile organic P (Po) that averaging 30.9 and 68.2 mg kg-1, respectively. Organic acids produced during crop residue decomposition can promote the complexation of counter-ions and decouple and release unbound Pi from metal and alkali metal phosphates. Animal manure and cover crop residues also contain large amounts of soluble organic matter, and likely generate similar ligands. However, a high degree of heterogeneity in P spatial distribution in such amended fields, arising from variances in substrate physical forms ranging from slurries to dried solids, composition, and diverse application methods and equipment. Distinct clusters of Pi and Po were observed, where accumulation of the latter forms was associated with high soil microbial biomass C and reduced phosphomonoesterases' activity. Accurate estimates of plant requirements and lability of soil P pools, and real-time plant and soil P sensing systems are critical considerations to optimally manage manure-derived nutrients in crop production systems. An in situ X-ray fluorescence-based approach to sensing canopy and soil XRFS-P was developed to improve the yield-soil P relationship for optimal nutrient recommendations in addition to allowing in-the-field verification of foliar P status.

Exponential Nutrient Loading as a Means to Optimize Bareroot Nursery Fertility of Oak Species

Treesearch

Zonda K. D. Birge; Douglass F. Jacobs; Francis K. Salifu

2006-01-01

Conventional fertilization in nursery culture of hardwoods may involve supply of equal fertilizer doses at regularly spaced intervals during the growing season, which may create a surplus of available nutrients in the beginning and a deficiency in nutrient availability by the end of the growing season. A method of fertilization termed “exponential nutrient loading” has...

Evolutionary Divergences in Root Exudate Composition among Ecologically-Contrasting Helianthus Species

PubMed Central

Bowsher, Alan W.; Ali, Rifhat; Harding, Scott A.; Tsai, Chung-Jui; Donovan, Lisa A.

2016-01-01

Plant roots exude numerous metabolites into the soil that influence nutrient availability. Although root exudate composition is hypothesized to be under selection in low fertility soils, few studies have tested this hypothesis in a phylogenetic framework. In this study, we examined root exudates of three pairs of Helianthus species chosen as phylogenetically-independent contrasts with respect to native soil nutrient availability. Under controlled environmental conditions, seedlings were grown to the three-leaf-pair stage, then transferred to either high or low nutrient treatments. After five days of nutrient treatments, we used gas chromatography-mass spectrometry for analysis of root exudates, and detected 37 metabolites across species. When compared in the high nutrient treatment, species native to low nutrient soils exhibited overall higher exudation than their sister species native to high nutrient soils in all three species pairs, providing support for repeated evolutionary shifts in response to native soil fertility. Species native to low nutrient soils and those native to high nutrient soils responded similarly to low nutrient treatments with increased exudation of organic acids (fumaric, citric, malic acids) and glucose, potentially as a mechanism to enhance nutrition acquisition. However, species native to low nutrient soils also responded to low nutrient treatments with a larger decrease in exudation of amino acids than species native to high nutrient soils in all three species pairs. This indicates that species native to low nutrient soils have evolved a unique sensitivity to changes in nutrient availability for some, but not all, root exudates. Overall, these repeated evolutionary divergences between species native to low nutrient soils and those native to high nutrient soils provide evidence for the adaptive value of root exudation, and its plasticity, in contrasting soil environments. PMID:26824236

Evolutionary Divergences in Root Exudate Composition among Ecologically-Contrasting Helianthus Species.

PubMed

Bowsher, Alan W; Ali, Rifhat; Harding, Scott A; Tsai, Chung-Jui; Donovan, Lisa A

2016-01-01

Plant roots exude numerous metabolites into the soil that influence nutrient availability. Although root exudate composition is hypothesized to be under selection in low fertility soils, few studies have tested this hypothesis in a phylogenetic framework. In this study, we examined root exudates of three pairs of Helianthus species chosen as phylogenetically-independent contrasts with respect to native soil nutrient availability. Under controlled environmental conditions, seedlings were grown to the three-leaf-pair stage, then transferred to either high or low nutrient treatments. After five days of nutrient treatments, we used gas chromatography-mass spectrometry for analysis of root exudates, and detected 37 metabolites across species. When compared in the high nutrient treatment, species native to low nutrient soils exhibited overall higher exudation than their sister species native to high nutrient soils in all three species pairs, providing support for repeated evolutionary shifts in response to native soil fertility. Species native to low nutrient soils and those native to high nutrient soils responded similarly to low nutrient treatments with increased exudation of organic acids (fumaric, citric, malic acids) and glucose, potentially as a mechanism to enhance nutrition acquisition. However, species native to low nutrient soils also responded to low nutrient treatments with a larger decrease in exudation of amino acids than species native to high nutrient soils in all three species pairs. This indicates that species native to low nutrient soils have evolved a unique sensitivity to changes in nutrient availability for some, but not all, root exudates. Overall, these repeated evolutionary divergences between species native to low nutrient soils and those native to high nutrient soils provide evidence for the adaptive value of root exudation, and its plasticity, in contrasting soil environments.

Brain nuclear receptors and body weight regulation

USDA-ARS?s Scientific Manuscript database

Neural pathways, especially those in the hypothalamus, integrate multiple nutritional, hormonal, and neural signals, resulting in the coordinated control of body weight balance and glucose homeostasis. Nuclear receptors (NRs) sense changing levels of nutrients and hormones, and therefore play essent...

Assessment of Evapotranspiration and Soil Moisture Content Across Different Scales of Observation

PubMed Central

Verstraeten, Willem W.; Veroustraete, Frank; Feyen, Jan

2008-01-01

The proper assessment of evapotranspiration and soil moisture content are fundamental in food security research, land management, pollution detection, nutrient flows, (wild-) fire detection, (desert) locust, carbon balance as well as hydrological modelling; etc. This paper takes an extensive, though not exhaustive sample of international scientific literature to discuss different approaches to estimate land surface and ecosystem related evapotranspiration and soil moisture content. This review presents: (i)a summary of the generally accepted cohesion theory of plant water uptake and transport including a shortlist of meteorological and plant factors influencing plant transpiration;(ii)a summary on evapotranspiration assessment at different scales of observation (sap-flow, porometer, lysimeter, field and catchment water balance, Bowen ratio, scintillometer, eddy correlation, Penman-Monteith and related approaches);(iii)a summary on data assimilation schemes conceived to estimate evapotranspiration using optical and thermal remote sensing; and(iv)for soil moisture content, a summary on soil moisture retrieval techniques at different spatial and temporal scales is presented. Concluding remarks on the best available approaches to assess evapotranspiration and soil moisture content with and emphasis on remote sensing data assimilation, are provided. PMID:27879697

Assessment of Evapotranspiration and Soil Moisture Content Across Different Scales of Observation.

PubMed

Verstraeten, Willem W; Veroustraete, Frank; Feyen, Jan

2008-01-09

The proper assessment of evapotranspiration and soil moisture content arefundamental in food security research, land management, pollution detection, nutrient flows,(wild-) fire detection, (desert) locust, carbon balance as well as hydrological modelling; etc.This paper takes an extensive, though not exhaustive sample of international scientificliterature to discuss different approaches to estimate land surface and ecosystem relatedevapotranspiration and soil moisture content. This review presents:(i) a summary of the generally accepted cohesion theory of plant water uptake andtransport including a shortlist of meteorological and plant factors influencing planttranspiration;(ii) a summary on evapotranspiration assessment at different scales of observation (sapflow,porometer, lysimeter, field and catchment water balance, Bowen ratio,scintillometer, eddy correlation, Penman-Monteith and related approaches);(iii) a summary on data assimilation schemes conceived to estimate evapotranspirationusing optical and thermal remote sensing; and(iv) for soil moisture content, a summary on soil moisture retrieval techniques atdifferent spatial and temporal scales is presented.Concluding remarks on the best available approaches to assess evapotranspiration and soilmoisture content with and emphasis on remote sensing data assimilation, are provided.

Evaluating the potential for watershed restoration to reduce nutrient loading to Upper Klamath Lake, Oregon

USGS Publications Warehouse

McCormick, Paul V.; Campbell, Sharon G.

2007-01-01

A literature review of best management practices to reduce nutrient loading was performed to provide information for resource managers in the Klamath Basin, Oregon. Although BMPs have already been implemented in the watershed, some sense of their effectiveness in reducing phosphorus loading and their cost for installation and maintenance is still lacking. This report discusses both causes of nutrient loading and a wide-variety of BMPs used to treat or reduce causal factors. We specifically focused on cattle grazing as the principal land-use and causal factor for nutrient loading in the Klamath Basin above Upper Klamath Lake, Oregon. Several BMP types, including stream corridor fencing, riparian buffer strips and constructed wetlands, seem to have potential for reducing phosphorus loading that may result from cattle grazing. However, no single BMP is likely to be the most effective in all locations or situations.

Adaptive Roles of SSY1 and SIR3 During Cycles of Growth and Starvation in Saccharomyces cerevisiae Populations Enriched for Quiescent or Nonquiescent Cells.

PubMed

Wloch-Salamon, Dominika M; Tomala, Katarzyna; Aggeli, Dimitra; Dunn, Barbara

2017-06-07

Over its evolutionary history, Saccharomyces cerevisiae has evolved to be well-adapted to fluctuating nutrient availability. In the presence of sufficient nutrients, yeast cells continue to proliferate, but upon starvation haploid yeast cells enter stationary phase and differentiate into nonquiescent (NQ) and quiescent (Q) cells. Q cells survive stress better than NQ cells and show greater viability when nutrient-rich conditions are restored. To investigate the genes that may be involved in the differentiation of Q and NQ cells, we serially propagated yeast populations that were enriched for either only Q or only NQ cell types over many repeated growth-starvation cycles. After 30 cycles (equivalent to 300 generations), each enriched population produced a higher proportion of the enriched cell type compared to the starting population, suggestive of adaptive change. We also observed differences in each population's fitness suggesting possible tradeoffs: clones from NQ lines were better adapted to logarithmic growth, while clones from Q lines were better adapted to starvation. Whole-genome sequencing of clones from Q- and NQ-enriched lines revealed mutations in genes involved in the stress response and survival in limiting nutrients ( ECM21 , RSP5 , MSN1 , SIR4 , and IRA2 ) in both Q and NQ lines, but also differences between the two lines: NQ line clones had recurrent independent mutations affecting the Ssy1p-Ptr3p-Ssy5p (SPS) amino acid sensing pathway, while Q line clones had recurrent, independent mutations in SIR3 and FAS1 Our results suggest that both sets of enriched-cell type lines responded to common, as well as distinct, selective pressures. Copyright © 2017 Wloch-Salamon et al.

Impact of Temperature and Nutrients on Carbon: Nutrient Tissue Stoichiometry of Submerged Aquatic Plants: An Experiment and Meta-Analysis.

PubMed

Velthuis, Mandy; van Deelen, Emma; van Donk, Ellen; Zhang, Peiyu; Bakker, Elisabeth S

2017-01-01

Human activity is currently changing our environment rapidly, with predicted temperature increases of 1-5°C over the coming century and increased nitrogen and phosphorus inputs in aquatic ecosystems. In the shallow parts of these ecosystems, submerged aquatic plants enhance water clarity by resource competition with phytoplankton, provide habitat, and serve as a food source for other organisms. The carbon:nutrient stoichiometry of submerged aquatic plants can be affected by changes in both temperature and nutrient availability. We hypothesized that elevated temperature leads to higher carbon:nutrient ratios through enhanced nutrient-use efficiency, while nutrient addition leads to lower carbon:nutrient ratios by the luxurious uptake of nutrients. We addressed these hypotheses with an experimental and a meta-analytical approach. We performed a full-factorial microcosm experiment with the freshwater plant Elodea nuttallii grown at 10, 15, 20, and 25°C on sediment consisting of pond soil/sand mixtures with 100, 50, 25, and 12.5% pond soil. To address the effect of climatic warming and nutrient addition on the carbon:nutrient stoichiometry of submerged freshwater and marine plants we performed a meta-analysis on experimental studies that elevated temperature and/or added nutrients (nitrogen and phosphorus). In the microcosm experiment, C:N ratios of Elodea nuttallii decreased with increasing temperature, and this effect was most pronounced at intermediate nutrient availability. Furthermore, higher nutrient availability led to decreased aboveground C:P ratios. In the meta-analysis, nutrient addition led to a 25, 22, and 16% reduction in aboveground C:N and C:P ratios and belowground C:N ratios, accompanied with increased N content. No consistent effect of elevated temperature on plant stoichiometry could be observed, as very few studies were found on this topic and contrasting results were reported. We conclude that while nutrient addition consistently leads to decreased carbon:nutrient ratios, elevated temperature does not change submerged aquatic plant carbon:nutrient stoichiometry in a consistent manner. This effect is rather dependent on nutrient availability and may be species-specific. As changes in the carbon:nutrient stoichiometry of submerged aquatic plants can impact the transfer of energy to higher trophic levels, these results suggest that eutrophication may enhance plant consumption and decomposition, which could in turn have consequences for carbon sequestration.

Regulation of phosphate starvation responses in higher plants.

PubMed

Yang, Xiao Juan; Finnegan, Patrick M

2010-04-01

Phosphorus (P) is often a limiting mineral nutrient for plant growth. Many soils worldwide are deficient in soluble inorganic phosphate (P(i)), the form of P most readily absorbed and utilized by plants. A network of elaborate developmental and biochemical adaptations has evolved in plants to enhance P(i) acquisition and avoid starvation. Controlling the deployment of adaptations used by plants to avoid P(i) starvation requires a sophisticated sensing and regulatory system that can integrate external and internal information regarding P(i) availability. In this review, the current knowledge of the regulatory mechanisms that control P(i) starvation responses and the local and long-distance signals that may trigger P(i) starvation responses are discussed. Uncharacterized mutants that have P(i)-related phenotypes and their potential to give us additional insights into regulatory pathways and P(i) starvation-induced signalling are also highlighted and assessed. An impressive list of factors that regulate P(i) starvation responses is now available, as is a good deal of knowledge regarding the local and long-distance signals that allow a plant to sense and respond to P(i) availability. However, we are only beginning to understand how these factors and signals are integrated with one another in a regulatory web able to control the range of responses demonstrated by plants grown in low P(i) environments. Much more knowledge is needed in this agronomically important area before real gains can be made in improving P(i) acquisition in crop plants.

Root Cortical Senescence Improves Growth under Suboptimal Availability of N, P, and K1[OPEN

PubMed Central

Schneider, Hannah M.

2017-01-01

Root cortical senescence (RCS) in Triticeae reduces nutrient uptake, nutrient content, respiration, and radial hydraulic conductance of root tissue. We used the functional-structural model SimRoot to evaluate the functional implications of RCS in barley (Hordeum vulgare) under suboptimal nitrate, phosphorus, and potassium availability. The utility of RCS was evaluated using sensitivity analyses in contrasting nutrient regimes. At flowering (80 d), RCS increased simulated plant growth by up to 52%, 73%, and 41% in nitrate-, phosphorus-, and potassium-limiting conditions, respectively. Plants with RCS had reduced nutrient requirement of root tissue for optimal plant growth, reduced total cumulative cortical respiration, and increased total carbon reserves. Nutrient reallocation during RCS had a greater effect on simulated plant growth than reduced respiration or nutrient uptake. Under low nutrient availability, RCS had greater benefit in plants with fewer tillers. RCS had greater benefit in phenotypes with fewer lateral roots at low nitrate availability, but the opposite was true in low phosphorus or potassium availability. Additionally, RCS was quantified in field-grown barley in different nitrogen regimes. Field and virtual soil coring simulation results demonstrated that living cortical volume per root length (an indicator of RCS) decreased with depth in younger plants, while roots of older plants had very little living cortical volume per root length. RCS may be an adaptive trait for nutrient acquisition by reallocating nutrients from senescing tissue and secondarily by reducing root respiration. These simulated results suggest that RCS merits investigation as a breeding target for enhanced soil resource acquisition and edaphic stress tolerance. PMID:28667049

Automated Liquid-Level Control of a Nutrient Reservoir for a Hydroponic System

NASA Technical Reports Server (NTRS)

Smith, Boris; Asumadu, Johnson A.; Dogan, Numan S.

1997-01-01

A microprocessor-based system for control of the liquid level of a nutrient reservoir for a plant hydroponic growing system has been developed. The system uses an ultrasonic transducer to sense the liquid level or height. A National Instruments' Multifunction Analog and Digital Input/Output PC Kit includes NI-DAQ DOS/Windows driver software for an IBM 486 personal computer. A Labview Full Development system for Windows is the graphical programming system being used. The system allows liquid level control to within 0.1 cm for all levels tried between 8 and 36 cm in the hydroponic system application. The detailed algorithms have been developed and a fully automated microprocessor based nutrient replenishment system has been described for this hydroponic system.

A survey for the use of remote sensing in the Chesapeake Bay region

NASA Technical Reports Server (NTRS)

Ulanowicz, R. E.

1974-01-01

Environmental problem areas concerning the Chesapeake Bay region are reviewed along with ongoing remote sensing programs pertaining to these problems, and recommendations are presented to help fill lacunae in present research and to utilize the remote sensing capabilities of NASA to their fullest. A list of interested organizations and individuals is presented for each category. The development of technologies to monitor dissolved nutrients in bay waters, the initiation of a census of the disappearing rooted acquatic plants in the littoral zones, and the mapping of natural building constraints in the growth regions of the states of Maryland and Virginia are among the recommendations presented.

Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

USDA-ARS?s Scientific Manuscript database

The mucosal barriers of catfish (Ictalurus spp.) constitute the first line of defense against pathogen invasion while simultaneously carrying out a diverse array of other critical physiological processes, including nutrient adsorption, osmoregulation, waste excretion, and environmental sensing. Catf...

Integration of Carbon, Nitrogen, and Oxygen Metabolism in Escherichia coli--Final Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rabinowitz, Joshua D; Wingreen, Ned s; Rabitz, Herschel A

2012-10-22

A key challenge for living systems is balancing utilization of multiple elemental nutrients, such as carbon, nitrogen, and oxygen, whose availability is subject to environmental fluctuations. As growth can be limited by the scarcity of any one nutrient, the rate at which each nutrient is assimilated must be sensitive not only to its own availability, but also to that of other nutrients. Remarkably, across diverse nutrient conditions, E. coli grows nearly optimally, balancing effectively the conversion of carbon into energy versus biomass. To investigate the link between the metabolism of different nutrients, we quantified metabolic responses to nutrient perturbations usingmore » LC-MS based metabolomics and built differential equation models that bridge multiple nutrient systems. We discovered that the carbonaceous substrate of nitrogen assimilation, -ketoglutarate, directly inhibits glucose uptake and that the upstream glycolytic metabolite, fructose-1,6-bisphosphate, ultrasensitively regulates anaplerosis to allow rapid adaptation to changing carbon availability. We also showed that NADH controls the metabolic response to changing oxygen levels. Our findings support a general mechanism for nutrient integration: limitation for a nutrient other than carbon leads to build-up of the most closely related product of carbon metabolism, which in turn feedback inhibits further carbon uptake.« less

Effects of poultry litter injection on ammonia volatilization, nitrogen availability, and nutrient losses in runoff

USDA-ARS?s Scientific Manuscript database

Poultry litter is a common organic amendment in agricultural production systems, but nutrient losses can reduce the effectiveness as a fertilizer. Three studies were conducted to determine differences in nutrient availability and loss when comparing injection and surface application. These investi...

Soil nutrient availability and reproductive effort drive patterns in nutrient resorption in Pentachlethra macroloba

Treesearch

K. L. Tully; Tana Wood; A. M. Schwantes; D. Lawrence

2013-01-01

The removal of nutrients from senescing tissues, nutrient resorption, is a key strategy for conserving nutrients in plants. However, our understanding of what drives patterns of nutrient resorption in tropical trees is limited. We examined the effects of nutrient sources (stand-level and tree-level soil fertility) and sinks (reproductive effort) on nitrogen (N) and...

Final Report: Connecting genomic capabilities to physiology and response: Systems biology of the widespread alga Micromonas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Worden, Alexandra Z.; Callister, Stephen; Stuart, Joshua

Increased stratification, less mixing and reduced nutrient concentrations in marine surface waters are predicted under a number of climate-change scenarios. These conditions are considered favorable for tiny photosynthetic algae (picophytoplankton), shaping their role in mediating future CO2 conditions. One possibility is that picophytoplankton such as Micromonas that have broad geographical ranges will more successfully adapt to changing environmental conditions. However, their capacity to thrive under the multi-factorial impacts of low pH, low nutrients, increasing temperature and changes in community composition is not known. Here, we developed the dual-Micromonas model system, which entailed generating optimized genomic information for two Micromonas speciesmore » and developing a highperformance chemostat system in which both CO2 and nutrients could be consistently manipulated. This system is now fully operational. Project results are available in several publications will others are still in the analysis phase. Overall, our results show that Micromonas primary production will likely decrease under predicted future climate conditions. Furthermore, our studies on Micromonas provide new insights to the land plant ancestor, including the discovery of conserved signaling mechanisms (known to be essential to plant development) as well as the discovery of widespread chemical-sensing molecular switches. Collectively, this research highlights Micromonas as an important new model green alga for understanding plant gene networks and evolution as well as for investigating perturbation effects on marine primary production.« less

Plant Survival in a Changing Environment: The Role of Nitric Oxide in Plant Responses to Abiotic Stress

PubMed Central

Simontacchi, Marcela; Galatro, Andrea; Ramos-Artuso, Facundo; Santa-María, Guillermo E.

2015-01-01

Nitric oxide in plants may originate endogenously or come from surrounding atmosphere and soil. Interestingly, this gaseous free radical is far from having a constant level and varies greatly among tissues depending on a given plant’s ontogeny and environmental fluctuations. Proper plant growth, vegetative development, and reproduction require the integration of plant hormonal activity with the antioxidant network, as well as the maintenance of concentration of reactive oxygen and nitrogen species within a narrow range. Plants are frequently faced with abiotic stress conditions such as low nutrient availability, salinity, drought, high ultraviolet (UV) radiation and extreme temperatures, which can influence developmental processes and lead to growth restriction making adaptive responses the plant’s priority. The ability of plants to respond and survive under environmental-stress conditions involves sensing and signaling events where nitric oxide becomes a critical component mediating hormonal actions, interacting with reactive oxygen species, and modulating gene expression and protein activity. This review focuses on the current knowledge of the role of nitric oxide in adaptive plant responses to some specific abiotic stress conditions, particularly low mineral nutrient supply, drought, salinity and high UV-B radiation. PMID:26617619

Analysis of field-scale spatial correlations and variations of soil nutrients using geostatistics.

PubMed

Liu, Ruimin; Xu, Fei; Yu, Wenwen; Shi, Jianhan; Zhang, Peipei; Shen, Zhenyao

2016-02-01

Spatial correlations and soil nutrient variations are important for soil nutrient management. They help to reduce the negative impacts of agricultural nonpoint source pollution. Based on the sampled available nitrogen (AN), available phosphorus (AP), and available potassium (AK), soil nutrient data from 2010, the spatial correlation, was analyzed, and the probabilities of the nutrient's abundance or deficiency were discussed. This paper presents a statistical approach to spatial analysis, the spatial correlation analysis (SCA), which was originally developed for describing heterogeneity in the presence of correlated variation and based on ordinary kriging (OK) results. Indicator kriging (IK) was used to assess the susceptibility of excess of soil nutrients based on crop needs. The kriged results showed there was a distinct spatial variability in the concentration of all three soil nutrients. High concentrations of these three soil nutrients were found near Anzhou. As the distance from the center of town increased, the concentration of the soil nutrients gradually decreased. Spatially, the relationship between AN and AP was negative, and the relationship between AP and AK was not clear. The IK results showed that there were few areas with a risk of AN and AP overabundance. However, almost the entire study region was at risk of AK overabundance. Based on the soil nutrient distribution results, it is clear that the spatial variability of the soil nutrients differed throughout the study region. This spatial soil nutrient variability might be caused by different fertilizer types and different fertilizing practices.

Remote sensing of vegetation structure using computer vision

NASA Astrophysics Data System (ADS)

Dandois, Jonathan P.

High-spatial resolution measurements of vegetation structure are needed for improving understanding of ecosystem carbon, water and nutrient dynamics, the response of ecosystems to a changing climate, and for biodiversity mapping and conservation, among many research areas. Our ability to make such measurements has been greatly enhanced by continuing developments in remote sensing technology---allowing researchers the ability to measure numerous forest traits at varying spatial and temporal scales and over large spatial extents with minimal to no field work, which is costly for large spatial areas or logistically difficult in some locations. Despite these advances, there remain several research challenges related to the methods by which three-dimensional (3D) and spectral datasets are joined (remote sensing fusion) and the availability and portability of systems for frequent data collections at small scale sampling locations. Recent advances in the areas of computer vision structure from motion (SFM) and consumer unmanned aerial systems (UAS) offer the potential to address these challenges by enabling repeatable measurements of vegetation structural and spectral traits at the scale of individual trees. However, the potential advances offered by computer vision remote sensing also present unique challenges and questions that need to be addressed before this approach can be used to improve understanding of forest ecosystems. For computer vision remote sensing to be a valuable tool for studying forests, bounding information about the characteristics of the data produced by the system will help researchers understand and interpret results in the context of the forest being studied and of other remote sensing techniques. This research advances understanding of how forest canopy and tree 3D structure and color are accurately measured by a relatively low-cost and portable computer vision personal remote sensing system: 'Ecosynth'. Recommendations are made for optimal conditions under which forest structure measurements should be obtained with UAS-SFM remote sensing. Ultimately remote sensing of vegetation by computer vision offers the potential to provide an 'ecologist's eye view', capturing not only canopy 3D and spectral properties, but also seeing the trees in the forest and the leaves on the trees.

The Governor has a sweet tooth - mouth sensing of nutrients to enhance sports performance.

PubMed

Burke, Louise M; Maughan, Ronald J

2015-01-01

The oral-pharyngeal cavity and the gastrointestinal tract are richly endowed with receptors that respond to taste, temperature and to a wide range of specific nutrient and non-nutritive food components. Ingestion of carbohydrate-containing drinks has been shown to enhance endurance exercise performance, and these responses have been attributed to post-absorptive effects. It is increasingly recognised, though, that the response to ingested carbohydrate begins in the mouth via specific carbohydrate receptors and continues in the gut via the release of a range of hormones that influence substrate metabolism. Cold drinks can also enhance performance, especially in conditions of thermal stress, and part of the mechanism underlying this effect may be the response to cold fluids in the mouth. There is also some, albeit not entirely consistent, evidence for effects of caffeine, quinine, menthol and acetic acid on performance or other relevant effects. This review summarises current knowledge of responses to mouth sensing of temperature, carbohydrate and other food components, with the goal of assisting athletes to implement practical strategies that make best use of its effects. It also examines the evidence that oral intake of other nutrients or characteristics associated with food/fluid intake during exercise can enhance performance via communication between the mouth/gut and the brain.

Climate and infectious disease: use of remote sensing for detection of Vibrio cholerae by indirect measurement

NASA Technical Reports Server (NTRS)

Lobitz, B.; Beck, L.; Huq, A.; Wood, B.; Fuchs, G.; Faruque, A. S.; Colwell, R.

2000-01-01

It has long been known that cholera outbreaks can be initiated when Vibrio cholerae, the bacterium that causes cholera, is present in drinking water in sufficient numbers to constitute an infective dose, if ingested by humans. Outbreaks associated with drinking or bathing in unpurified river or brackish water may directly or indirectly depend on such conditions as water temperature, nutrient concentration, and plankton production that may be favorable for growth and reproduction of the bacterium. Although these environmental parameters have routinely been measured by using water samples collected aboard research ships, the available data sets are sparse and infrequent. Furthermore, shipboard data acquisition is both expensive and time-consuming. Interpolation to regional scales can also be problematic. Although the bacterium, V. cholerae, cannot be sensed directly, remotely sensed data can be used to infer its presence. In the study reported here, satellite data were used to monitor the timing and spread of cholera. Public domain remote sensing data for the Bay of Bengal were compared directly with cholera case data collected in Bangladesh from 1992-1995. The remote sensing data included sea surface temperature and sea surface height. It was discovered that sea surface temperature shows an annual cycle similar to the cholera case data. Sea surface height may be an indicator of incursion of plankton-laden water inland, e.g., tidal rivers, because it was also found to be correlated with cholera outbreaks. The extensive studies accomplished during the past 25 years, confirming the hypothesis that V. cholerae is autochthonous to the aquatic environment and is a commensal of zooplankton, i.e., copepods, when combined with the findings of the satellite data analyses, provide strong evidence that cholera epidemics are climate-linked.

Food crop production, nutrient availability, and nutrient intakes in Bangladesh: exploring the agriculture-nutrition nexus with the 2010 Household Income and Expenditure Survey.

PubMed

Fiedler, John L

2014-12-01

Systematic collection of national agricultural data has been neglected in many low- and middle-income countries for the past 20 years. Commonly conducted nationally representative household surveys collect substantial quantities of highly underutilized food crop production data. To demonstrate the potential usefulness of commonly available household survey databases for analyzing the agriculture-nutrition nexus. Using household data from the 2010 Bangladesh Household Income and Expenditure Survey, the role and significance of crop selection, area planted, yield, nutrient production, and the disposition of 34 food crops in affecting the adequacy of farming households' nutrient availability and nutrient intake status are explored. The adequacy of each farming household's available energy, vitamin A, calcium, iron, and zinc and households' apparent intakes and intake adequacies are estimated. Each household's total apparent nutrient intake adequacies are estimated, taking into account the amount of each crop that households consume from their own production, together with food purchased or obtained from other sources. Even though rice contains relatively small amounts of micronutrients, has relatively low nutrient density, and is a relatively poor source of nutrients compared with what other crops can produce on a given tract of land, because so much rice is produced in Bangladesh, it is the source of 90% of the total available energy, 85% of the zinc, 67% of the calcium, and 55% of the iron produced by the agricultural sector. The domination of agriculture and diet by rice is a major constraint to improving nutrition in Bangladesh. Simple examples of how minor changes in the five most common cropping patterns could improve farming households' nutritional status are provided. Household surveys' agricultural modules can provide a useful tool for better understanding national nutrient production realities and possibilities.

The GPRC6A receptor displays constitutive internalization and sorting to the slow recycling pathway.

PubMed

Jacobsen, Stine Engesgaard; Ammendrup-Johnsen, Ina; Jansen, Anna Mai; Gether, Ulrik; Madsen, Kenneth Lindegaard; Bräuner-Osborne, Hans

2017-04-28

The class C G protein-coupled receptor GPRC6A is a putative nutrient-sensing receptor and represents a possible new drug target in metabolic disorders. However, the specific physiological role of this receptor has yet to be identified, and the mechanisms regulating its activity and cell surface availability also remain enigmatic. In the present study, we investigated the trafficking properties of GPRC6A by use of both a classical antibody feeding internalization assay in which cells were visualized using confocal microscopy and a novel internalization assay that is based on real-time measurements of fluorescence resonance energy transfer. Both assays revealed that GPRC6A predominantly undergoes constitutive internalization, whereas the agonist-induced effects were imperceptible. Moreover, postendocytic sorting was investigated by assessing the co-localization of internalized GPRC6A with selected Rab protein markers. Internalized GPRC6A was mainly co-localized with the early endosome marker Rab5 and the long loop recycling endosome marker Rab11 and to a much lesser extent with the late endosome marker Rab7. This suggests that upon agonist-independent internalization, GPRC6A is recycled via the Rab11-positive slow recycling pathway, which may be responsible for ensuring a persistent pool of GPRC6A receptors at the cell surface despite chronic agonist exposure. Distinct trafficking pathways have been reported for several of the class C receptors, and our results thus substantiate that non-canonical trafficking mechanisms are a common feature for the nutrient-sensing class C family that ensure functional receptors in the cell membrane despite prolonged agonist exposure. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

The GPRC6A receptor displays constitutive internalization and sorting to the slow recycling pathway

PubMed Central

Jacobsen, Stine Engesgaard; Ammendrup-Johnsen, Ina; Jansen, Anna Mai; Gether, Ulrik; Madsen, Kenneth Lindegaard; Bräuner-Osborne, Hans

2017-01-01

The class C G protein-coupled receptor GPRC6A is a putative nutrient-sensing receptor and represents a possible new drug target in metabolic disorders. However, the specific physiological role of this receptor has yet to be identified, and the mechanisms regulating its activity and cell surface availability also remain enigmatic. In the present study, we investigated the trafficking properties of GPRC6A by use of both a classical antibody feeding internalization assay in which cells were visualized using confocal microscopy and a novel internalization assay that is based on real-time measurements of fluorescence resonance energy transfer. Both assays revealed that GPRC6A predominantly undergoes constitutive internalization, whereas the agonist-induced effects were imperceptible. Moreover, postendocytic sorting was investigated by assessing the co-localization of internalized GPRC6A with selected Rab protein markers. Internalized GPRC6A was mainly co-localized with the early endosome marker Rab5 and the long loop recycling endosome marker Rab11 and to a much lesser extent with the late endosome marker Rab7. This suggests that upon agonist-independent internalization, GPRC6A is recycled via the Rab11-positive slow recycling pathway, which may be responsible for ensuring a persistent pool of GPRC6A receptors at the cell surface despite chronic agonist exposure. Distinct trafficking pathways have been reported for several of the class C receptors, and our results thus substantiate that non-canonical trafficking mechanisms are a common feature for the nutrient-sensing class C family that ensure functional receptors in the cell membrane despite prolonged agonist exposure. PMID:28280242

Validation testing of a soil macronutrient sensing system

USDA-ARS?s Scientific Manuscript database

Rapid on-site measurements of soil macronutrients (i.e., nitrogen, phosphorus, and potassium) are needed for site-specific crop management, where fertilizer nutrient application rates are adjusted spatially based on local requirements. This study reports on validation testing of a previously develop...

Effects of burn temperature on ash nutrient forms and availability from cattail (Typha domingensis) and sawgrass (Cladium jamaicense) in the Florida Everglades.

PubMed

Qian, Y; Miao, S L; Gu, B; Li, Y C

2009-01-01

Plant ash derived from fire plays an important role in nutrient balance and cycling in ecosystems. Factors that determine the composition and availability of ash nutrients include fire intensity (burn temperature and duration), plant species, habitat nutrient enrichment, and leaf type (live or dead leaf). We used laboratory simulation methods to evaluate temperature effects on nutrient composition and metals in the residual ash of sawgrass (Cladium jamaicense) and cattail (Typha domingensis), particularly on post-fire phosphorus (P) availability in plant ash. Live and dead leaf samples were collected from Water Conservation Area 2A in the northern Everglades along a soil P gradient, where prescribed fire may be used to accelerate recovery of this unique ecosystem. Significant decreases in total carbon and total nitrogen were detected with increasing fire temperature. Organic matter combustion was nearly complete at temperatures > or = 450 degrees C. HCl-extractable P (average, 50% of total P in the ash) and NH(4)Cl-extractable P (average, 33% of total P in the ash) were the predominant P fractions for laboratory-burned ash. Although a low-intensity fire could induce an elevation of P availability, an intense fire generally resulted in decreased water-soluble P. Significant differences in nutrient compositions were observed between species, habitat nutrient status, and leaf types. More labile inorganic P remained in sawgrass ash than in cattail ash; hence, sawgrass ash has a greater potential to release available P than cattail. Fire intensity affected plant ash nutrient composition, particularly P availability, and the effects varied with plant species and leaf type. Therefore, it is important to consider fire intensity and vegetation community when using a prescribed fire for ecosystem management.

Plant Nutrition 2: Macronutrients (N, P, K, S, Mg, and Ca)

PubMed Central

2014-01-01

Summary In the second of three lessons spanning the topic of Plant Nutrition, we examine how macronutrients affect plant growth. Specifically, we look at (1) the availability of nutrients in the soil along with the effects of soil microbes and physical properties on their availability; (2) nutrient uptake from the external environment, across plasma membranes and into plant cells; (3) in some cases, the assimilation of the nutrient into organic molecules; (4) the distribution and redistribution of nutrients throughout the plant; and (5) regulation of these processes. In parallel, we examine the genetic basis of a plant's nutrient use efficiency (NUE) and evaluate strategies by which to replenish nutrients that growing plants extract from soil.

[Spatial variability of surface soil nutrients in the landslide area of Beichuan County, South- west China, after 5 · 12 Wenchuan Earthquake].

PubMed

Mai, Ji-shan; Zhao, Ting-ning; Zheng, Jiang-kun; Shi, Chang-qing

2015-12-01

Based on grid sampling and laboratory analysis, spatial variability of surface soil nutrients was analyzed with GS⁺ and other statistics methods on the landslide area of Fenghuang Mountain, Leigu Town, Beichuan County. The results showed that except for high variability of available phosphorus, other soil nutrients exhibited moderate variability. The ratios of nugget to sill of the soil available phosphorus and soil organic carbon were 27.9% and 28.8%, respectively, showing moderate spatial correlation, while the ratios of nugget to sill of the total nitrogen (20.0%), total phosphorus (24.3%), total potassium (11.1%), available nitrogen (11.2%), and available potassium (22.7%) suggested strong spatial correlation. The total phosphorus had the maximum range (1232.7 m), followed by available nitrogen (541.27 m), total nitrogen (468.35 m), total potassium (136.0 m), available potassium (128.7 m), available phosphorus (116.6 m), and soil organic carbon (93.5 m). Soil nutrients had no significant variation with the increase of altitude, but gradually increased from the landslide area, the transition area, to the little-impacted area. The total and available phosphorus contents of the landslide area decreased by 10.3% and 79.7% compared to that of the little-impacted area, respectively. The soil nutrient contents in the transition area accounted for 31.1%-87.2% of that of the little-impacted area, with the nant reason for the spatial variability of surface soil nutrients.

Unravelling the limits to tree height: a major role for water and nutrient trade-offs.

PubMed

Cramer, Michael D

2012-05-01

Competition for light has driven forest trees to grow exceedingly tall, but the lack of a single universal limit to tree height indicates multiple interacting environmental limitations. Because soil nutrient availability is determined by both nutrient concentrations and soil water, water and nutrient availabilities may interact in determining realised nutrient availability and consequently tree height. In SW Australia, which is characterised by nutrient impoverished soils that support some of the world's tallest forests, total [P] and water availability were independently correlated with tree height (r = 0.42 and 0.39, respectively). However, interactions between water availability and each of total [P], pH and [Mg] contributed to a multiple linear regression model of tree height (r = 0.72). A boosted regression tree model showed that maximum tree height was correlated with water availability (24%), followed by soil properties including total P (11%), Mg (10%) and total N (9%), amongst others, and that there was an interaction between water availability and total [P] in determining maximum tree height. These interactions indicated a trade-off between water and P availability in determining maximum tree height in SW Australia. This is enabled by a species assemblage capable of growing tall and surviving (some) disturbances. The mechanism for this trade-off is suggested to be through water enabling mass-flow and diffusive mobility of P, particularly of relatively mobile organic P, although water interactions with microbial activity could also play a role.

Pharyngeal sense organs drive robust sugar consumption in Drosophila

PubMed Central

LeDue, Emily E; Chen, Yu-Chieh; Jung, Aera Y; Dahanukar, Anupama; Gordon, Michael D

2015-01-01

The fly pharyngeal sense organs lie at the transition between external and internal nutrient sensing mechanisms. Here, we investigate the function of pharyngeal sweet gustatory receptor neurons (GRNs), demonstrating that they express a subset of the nine previously identified sweet receptors and respond to stimulation with a panel of sweet compounds. We show that pox-neuro (poxn) mutants lacking taste function in the legs and labial palps have intact pharyngeal sweet taste, which is both necessary and sufficient to drive preferred consumption of sweet compounds by prolonging ingestion. Moreover, flies putatively lacking all sweet taste show little preference for nutritive or non-nutritive sugars in a short-term feeding assay. Together, our data demonstrate that pharyngeal sense organs play an important role in directing sustained consumption of sweet compounds, and suggest that post-ingestive sugar sensing does not effectively drive food choice in a simple short-term feeding paradigm. PMID:25807033

Growth, allocation and tissue chemistry of Picea abies seedlings affected by nutrient supply during the second growing season.

PubMed

Kaakinen, Seija; Jolkkonen, Annika; Iivonen, Sari; Vapaavuori, Elina

2004-06-01

One-year-old Norway spruce (Picea abies (L.) Karst.) seedlings were grown hydroponically in a growth chamber to investigate the effects of low and high nutrient availability (LN; 0.25 mM N and HN; 2.50 mM N) on growth, biomass allocation and chemical composition of needles, stem and roots during the second growing season. Climatic conditions in the growth chamber simulated the mean growing season from May to early October in Flakaliden, northern Sweden. In the latter half of the growing season, biomass allocation changed in response to nutrient availability: increased root growth and decreased shoot growth led to higher root/shoot ratios in LN seedlings than in HN seedlings. At high nutrient availability, total biomass, especially stem biomass, increased, as did total nonstructural carbohydrate and nitrogen contents per seedling. Responses of stem chemistry to nutrient addition differed from those of adult trees of the same provenance. In HN seedlings, concentrations of alpha-cellulose, hemicellulose and lignin decreased in the secondary xylem. Our results illustrate the significance of retranslocation of stored nutrients to support new growth early in the season when root growth and nutrient uptake are still low. We conclude that nutrient availability alters allocation patterns, thereby influencing the success of 2-year-old Norway spruce seedlings at forest planting sites.

Elkhorn Slough: Detecting Eutrophication through Geospatial Modeling Applications

NASA Astrophysics Data System (ADS)

Caraballo Álvarez, I. O.; Childs, A.; Jurich, K.

2016-12-01

Elkhorn Slough in Monterey, California, has experienced substantial nutrient loading and eutrophication over the past 21 years as a result of fertilizer-rich runoff from nearby agricultural fields. This study seeks to identify and track spatial patterns of eutrophication hotspots and the correlation to land use changes, possible nutrient sources, and general climatic trends using remotely sensed and in situ data. Threats of rising sea level, subsiding marshes, and increased eutrophication hotspots demonstrate the necessity to analyze the effects of increasing nutrient loads, relative sea level changes, and sedimentation within Elkhorn Slough. The Soil & Water Assessment Tool (SWAT) model integrates specified inputs to assess nutrient and sediment loading and their sources. TerrSet's Land Change Modeler forecasts the future potential of land change transitions for various land cover classes around the slough as a result of nutrient loading, eutrophication, and increased sedimentation. TerrSet's Earth Trends Modeler provides a comprehensive analysis of image time series to rapidly assess long term eutrophication trends and detect spatial patterns of known hotspots. Results from this study will inform future coastal management practices and provide greater spatial and temporal insight into Elkhorn Slough eutrophication dynamics.

Maternal–Fetal Nutrient Transport in Pregnancy Pathologies: The Role of the Placenta

PubMed Central

Brett, Kendra Elizabeth; Ferraro, Zachary Michael; Yockell-Lelievre, Julien; Gruslin, Andrée; Adamo, Kristi Bree

2014-01-01

Appropriate in utero growth is essential for offspring development and is a critical contributor to long-term health. Fetal growth is largely dictated by the availability of nutrients in maternal circulation and the ability of these nutrients to be transported into fetal circulation via the placenta. Substrate flux across placental gradients is dependent on the accessibility and activity of nutrient-specific transporters. Changes in the expression and activity of these transporters is implicated in cases of restricted and excessive fetal growth, and may represent a control mechanism by which fetal growth rate attempts to match availability of nutrients in maternal circulation. This review provides an overview of placenta nutrient transport with an emphasis on macro-nutrient transporters. It highlights the changes in expression and activity of these transporters associated with common pregnancy pathologies, including intrauterine growth restriction, macrosomia, diabetes and obesity, as well as the potential impact of maternal diet. Molecular signaling pathways linking maternal nutrient availability and placenta nutrient transport are discussed. How sexual dimorphism affects fetal growth strategies and the placenta’s response to an altered intrauterine environment is considered. Further knowledge in this area may be the first step in the development of targeted interventions to help optimize fetal growth. PMID:25222554

Probiotics for Plants? Growth Promotion by the Entomopathogenic Fungus Beauveria bassiana Depends on Nutrient Availability.

PubMed

Tall, Susanna; Meyling, Nicolai V

2018-03-28

Cultivation of crops requires nutrient supplements which are costly and impact the environment. Furthermore, global demands for increased crop production call for sustainable solutions to increase yield and utilize resources such as nutrients more effectively. Some entomopathogenic fungi are able to promote plant growth, but studies over such effects have been conducted under optimal conditions where nutrients are abundantly available. We studied the effects of Beauveria bassiana (strain GHA) seed treatment on the growth of maize (Zea mays) at high and low nutrient conditions during 6 weeks in greenhouse. As expected, B. bassiana seed treatment increased plant growth, but only at high nutrient conditions. In contrast, the seed treatment did not benefit plant growth at low nutrient conditions where the fungus potentially constituted a sink and tended to reduce plant growth. The occurrence of endophytic B. bassiana in experimental plant tissues was evaluated by PCR after 6 weeks, but B. bassiana was not documented in any of the above-ground plant tissues indicating that the fungus-plant interaction was independent of endophytic establishment. Our results suggest that B. bassiana seed treatment could be used as a growth promoter of maize when nutrients are abundantly available, while the fungus does not provide any growth benefits when nutrients are scarce.

Hypothalamic mTOR signaling regulates food intake.

PubMed

Cota, Daniela; Proulx, Karine; Smith, Kathi A Blake; Kozma, Sara C; Thomas, George; Woods, Stephen C; Seeley, Randy J

2006-05-12

The mammalian Target of Rapamycin (mTOR) protein is a serine-threonine kinase that regulates cell-cycle progression and growth by sensing changes in energy status. We demonstrated that mTOR signaling plays a role in the brain mechanisms that respond to nutrient availability, regulating energy balance. In the rat, mTOR signaling is controlled by energy status in specific regions of the hypothalamus and colocalizes with neuropeptide Y and proopiomelanocortin neurons in the arcuate nucleus. Central administration of leucine increases hypothalamic mTOR signaling and decreases food intake and body weight. The hormone leptin increases hypothalamic mTOR activity, and the inhibition of mTOR signaling blunts leptin's anorectic effect. Thus, mTOR is a cellular fuel sensor whose hypothalamic activity is directly tied to the regulation of energy intake.

Improved Hypoxia Modeling for Nutrient Control Decisions in the Gulf of Mexico

NASA Technical Reports Server (NTRS)

Habib, Shahid; Pickering, Ken; Tzortziou, Maria; Maninio, Antonio; Policelli, Fritz; Stehr, Jeff

2011-01-01

The Gulf of Mexico Modeling Framework is a suite of coupled models linking the deposition and transport of sediment and nutrients to subsequent bio-geo chemical processes and the resulting effect on concentrations of dissolved oxygen in the coastal waters of Louisiana and Texas. Here, we examine the potential benefits of using multiple NASA remote sensing data products within this Modeling Framework for increasing the accuracy of the models and their utility for nutrient control decisions in the Gulf of Mexico. Our approach is divided into three components: evaluation and improvement of (a) the precipitation input data (b) atmospheric constituent concentrations in EPA's air quality/deposition model and (c) the calculation of algal biomass, organic carbon and suspended solids within the water quality/eutrophication models of the framework.

REMOTE SENSING FOR DETECTING SWINE ANIMAL FEEDING OPERATIONS

EPA Science Inventory

Surface runoff from animal feeding operations (AFO's) and its infiltration into ground water can
pose a number of risks to water quality mainly because of the amount of animal manure and wastewater they produce. Excess nutrients generated by livestock facilities can lead to a...

Nutrient cycling for biomass: Interactive proteomic/transcriptomic networks for global carbon management processes within poplar-mycorrhizal interactions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cseke, Leland

This project addresses the need to develop system-scale models at the symbiotic interface between ectomycorrhizal fungi (Laccaria bicolor) and tree species (Populus tremuloides) in response to environmental nutrient availability / biochemistry. Using our now well-established laboratory Laccaria x poplar system, we address the hypothesis that essential regulatory and metabolic mechanisms can be inferred from genomic, transcriptomic and proteomic-level changes that occur in response to environmental nutrient availability. The project addresses this hypothesis by applying state-of-the-art protein-level analytic approaches to fill the gap in our understanding of how mycorrhizal regulatory and metabolic processes at the transcript-level translate to nutrient uptake, carbonmore » management and ultimate net primary productivity of plants. In most cases, these techniques were not previously optimized for poplar trees or Laccaria. Thus, one of the major contributions of this project has been to provide avenues for new research in these species by overcoming the pitfalls that had previously prevented the use of techniques such as ChIP-Seq and SWATH-proteomics. Since it is the proteins that sense and interact with the environment, participate in signal cascades, activate and regulate gene expression, perform the activities of metabolism and ultimately sequester carbon and generate biomass, an understanding of protein activities during symbiosis-linked nutrient uptake is critical to any systems-level approach that links metabolic processes to the environment. This project uses a team of experts at The University of Alabama in Huntsville (UAH), The University of Alabama at Birmingham (UAB) and Argonne National Laboratory (ANL) to address the above hypothesis using a multiple "omics" approach that combines gene and protein expression as well as protein modifications, and biochemical analyses (performed at Brookhaven National Laboratory (BNL)) in poplar trees under mycorrhizal and free living conditions. Together, the assembled team of experts completed all of the planned milestones set forth in this project. In addition to the planned approaches, several lines of exciting new research have also evolved during the course of this project that involved FTIR Imaging using the National Synchrotron Light Source at BNL. A summary of the approaches used in this project and key highlights are as follows: Having the right combination of microbes associated with plants is largely responsible for the plant’s ability to mine nutrients from the soil and to develop a strong “immune system”. Our current chemically focused and intensive culture tends to forget that plants obtain nutrients in two ways: (1) via water soluble chemical nutrients and (2) via the activity of acquired microbial symbionts. In healthy natural ecosystems, chemical nutrients are always in low abundance because the organisms within that system have already locked such nutrients away within the biological system itself. Thus, in nature it is the biological sources of nutrients and the microbes that have the capacity to mine those nutrients for their plant hosts that actually control the terrestrial nutrient cycles on this planet. Thus, a new push in the future may very well be to use our skills at elucidating complex patterns to strategically guide soil microbe communities to do what we want, essentially allowing nature to do the work of figuring out what is most efficient and effective for human needs. However, the findings of this project and other work in our lab lead to the hypothesis that the specific soil community composition is less important than the emergent properties of those communities. So, additional research into what soil communities are effective and how they are established will be key in developing human understanding of how to manipulate biological systems to meet human needs without causing undue damage to our environment.« less

Effects of nutrient availabiiity on biomass allocation as well as constitutive and rapid induced herbivore resistance in poplar

Treesearch

Carolyn Glynn; Daniel A. Herms; Marie Egawa; Robert Hansen; William J. Mattson

2003-01-01

Many studies have examined effects of nutrient availability on constitutive herbivore resistance of plants, but few have addressed effects on expression of rapid induced resistance (RIR). We quantified effects of two levels of nutrient availability on growth, biomass allocation, photosynthesis, and constitutive secondary metabolism of black poplar (>i>Populus...

The influence of nutrient and water availability on carbohydrate storage in loblolly pine

Treesearch

K.H. Ludovici; H.L. Allen; T.J. Albaugh; P.M. Dougherty

2002-01-01

We quantified the effects of nutrient and water availability on monthly whole-tree carbohydrate budgets and determined allocation patterns of storage carbohydrates in loblolly pine (Pinus taeda) to test site resource impacts on internal carbon (C) storage. A factorial combination of two nutrient and two irrigation treatments were imposed on a 7-year...

[Spatial variability of soil nutrients based on geostatistics combined with GIS--a case study in Zunghua City of Hebei Province].

PubMed

Guo, X; Fu, B; Ma, K; Chen, L

2000-08-01

Geostatistics combined with GIS was applied to analyze the spatial variability of soil nutrients in topsoil (0-20 cm) in Zunghua City of Hebei Province. GIS can integrate attribute data with geographical data of system variables, which makes the application of geostatistics technique for large spatial scale more convenient. Soil nutrient data in this study included available N (alkaline hydrolyzing nitrogen), total N, available K, available P and organic matter. The results showed that the semivariograms of soil nutrients were best described by spherical model, except for that of available K, which was best fitted by complex structure of exponential model and linear with sill model. The spatial variability of available K was mainly produced by structural factor, while that of available N, total N, available P and organic matter was primarily caused by random factor. However, their spatial heterogeneity degree was different: the degree of total N and organic matter was higher, and that of available P and available N was lower. The results also indicated that the spatial correlation of the five tested soil nutrients at this large scale was moderately dependent. The ranges of available N and available P were almost same, which were 5 km and 5.5 km, respectively. The range of total N was up to 18 km, and that of organic matter was 8.5 km. For available K, the spatial variability scale primarily expressed exponential model between 0-3.5 km, but linear with sill model between 3.5-25.5 km. In addition, five soil nutrients exhibited different isotropic ranges. Available N and available P were isotropic through the whole research range (0-28 km). The isotropic range of available K was 0-8 km, and that of total N and organic matter was 0-10 km.

The availability and accessibility of nutrition information in fast food outlets in five states post-menu labelling legislation in New South Wales.

PubMed

Wellard, Lyndal; Havill, Michelle; Hughes, Clare; Watson, Wendy L; Chapman, Kathy

2015-12-01

1) Explore the availability and accessibility of fast food energy and nutrient information post-NSW menu labelling legislation in states with and without menu labelling legislation. 2) Determine whether availability and accessibility differed compared with pre-menu labelling legislation in NSW. We visited 210 outlets of the five largest fast food chains in five Australian states to observe the availability and accessibility of energy and nutrient information. Results were compared with 197 outlets surveyed pre-menu labelling. Most outlets (95%) provided energy values, half provided nutrient values and 3% provided information for all menu items. The total amount of information available increased post-NSW menu labelling implementation (473 versus 178 pre-implementation, p<0.001); however, fewer outlets provided nutrient values (26% versus 97% pre-implementation, p<0.001). Fast food chains surveyed had voluntarily introduced menu labelling nationally. However, more nutrient information was available in-store in 2010, showing that fast food chains are able to provide comprehensive nutrition information, yet they have stopped doing so. Menu labelling legislation should compel fast food chains to provide accessible nutrition information including nutrient values in addition to energy for all menu items in-store. Additionally, public education campaigns are needed to ensure customers can use menu labelling. © 2015 Public Health Association of Australia.

Selection of Optimal Auxiliary Soil Nutrient Variables for Cokriging Interpolation

PubMed Central

Song, Genxin; Zhang, Jing; Wang, Ke

2014-01-01

In order to explore the selection of the best auxiliary variables (BAVs) when using the Cokriging method for soil attribute interpolation, this paper investigated the selection of BAVs from terrain parameters, soil trace elements, and soil nutrient attributes when applying Cokriging interpolation to soil nutrients (organic matter, total N, available P, and available K). In total, 670 soil samples were collected in Fuyang, and the nutrient and trace element attributes of the soil samples were determined. Based on the spatial autocorrelation of soil attributes, the Digital Elevation Model (DEM) data for Fuyang was combined to explore the coordinate relationship among terrain parameters, trace elements, and soil nutrient attributes. Variables with a high correlation to soil nutrient attributes were selected as BAVs for Cokriging interpolation of soil nutrients, and variables with poor correlation were selected as poor auxiliary variables (PAVs). The results of Cokriging interpolations using BAVs and PAVs were then compared. The results indicated that Cokriging interpolation with BAVs yielded more accurate results than Cokriging interpolation with PAVs (the mean absolute error of BAV interpolation results for organic matter, total N, available P, and available K were 0.020, 0.002, 7.616, and 12.4702, respectively, and the mean absolute error of PAV interpolation results were 0.052, 0.037, 15.619, and 0.037, respectively). The results indicated that Cokriging interpolation with BAVs can significantly improve the accuracy of Cokriging interpolation for soil nutrient attributes. This study provides meaningful guidance and reference for the selection of auxiliary parameters for the application of Cokriging interpolation to soil nutrient attributes. PMID:24927129

Distributions of Competing Container Mosquitoes Depend on Detritus Types, Nutrient Ratios, and Food Availability

PubMed Central

Murrell, Ebony G.; Damal, Kavitha; Lounibos, L. P.; Juliano, Steven A.

2012-01-01

Coexistence of competitors may result if resources are sufficiently abundant to render competition unimportant, or if species differ in resource requirements. Detritus type has been shown to affect interspecific competitive outcomes between Aedes albopictus (Skuse) and Aedes aegypti (L.) larvae under controlled conditions. We assessed the relationships among spatial distributions of detritus types, nutrients, and aquatic larvae of these species in nature. We collected mosquitoes, water, and detritus from artificial containers across 24 Florida cemeteries that varied in relative abundances of Ae. aegypti and Ae. albopictus.We measured nutrient content of fine particulate organic matter in water samples as total N, P, and C and ratios of these nutrients. We quantified food availability via a bioassay, raising individual Aedes larvae in the laboratory in standard volumes of field-collected, particulate-containing water from each cemetery. Quantities of detritus types collected in standard containers were significant predictors of nutrients and nutrient ratios. Nutrient abundances were significant predictors of relative abundance of Ae. aegypti, and of larval survival and development by both species in the bioassay. Survival and development of larvae reared in particulate-containing water from sites decreased with decreasing relative abundance of Ae. aegypti. These data suggest that N, P, and C availabilities are determined by detritus inputs to containers and that these nutrients in turn determine the feeding environment encountered by larvae, the intensity of interspecific competition among larvae, and subsequent relative abundances of species at sites. Detritus inputs, nutrients, and food availability thus seem to contribute to distributions of Ae. aegypti and Ae. albopictus in cemetery containers throughout Florida. PMID:22707761

Growth of mature boreal Norway spruce was not affected by elevated [CO(2)] and/or air temperature unless nutrient availability was improved.

PubMed

Sigurdsson, Bjarni D; Medhurst, Jane L; Wallin, Göran; Eggertsson, Olafur; Linder, Sune

2013-11-01

The growth responses of mature Norway spruce (Picea abies (L.) Karst.) trees exposed to elevated [CO(2)] (CE; 670-700 ppm) and long-term optimized nutrient availability or elevated air temperature (TE; ±3.9 °C) were studied in situ in northern Sweden in two 3 year field experiments using 12 whole-tree chambers in ca. 40-year-old forest. The first experiment (Exp. I) studied the interactions between CE and nutrient availability and the second (Exp. II) between CE and TE. It should be noted that only air temperature was elevated in Exp. II, while soil temperature was maintained close to ambient. In Exp. I, CE significantly increased the mean annual height increment, stem volume and biomass increment during the treatment period (25, 28, and 22%, respectively) when nutrients were supplied. There was, however, no significant positive CE effect found at the low natural nutrient availability. In Exp. II, which was conducted at the natural site fertility, neither CE nor TE significantly affected height or stem increment. It is concluded that the low nutrient availability (mainly nitrogen) in the boreal forests is likely to restrict their response to the continuous rise in [CO(2)] and/or TE.

Modeling the role of quorum sensing in interspecies competition in biofilms

NASA Astrophysics Data System (ADS)

Narla, Avaneesh V.; Wingreen, Ned S.; Borenstein, David B.

Bacteria grow on surfaces in complex immobile communities known as biofilms, composed of cells embedded in an extracellular matrix. Within biofilms, bacteria often communicate, cooperate, and compete within their own species and with other species using Quorum Sensing (QS). QS refers to the process by which bacteria produce, secrete, and subsequently detect small molecules called autoinducers as a way to assess the local population density of their species, or of other species. QS is known to regulate the production of extracellular matrix. We investigated the possible benefit of QS in regulating matrix production to best gain access to a nutrient that diffuses from a source positioned away from the surface on which the biofilm grows. We employed Agent-Based Modeling (ABM), a form of simulation that allows cells to modify their behavior based on local inputs, e.g. nutrient and QS concentrations. We first determined the optimal fixed strategies (that do not use QS) for pairwise competitions, and then demonstrated that simple QS-based strategies can be superior to any fixed strategy. In nature, species can compete by sensing and/or interfering with each other's QS signals, and we explore approaches for targeting specific species via QS-interference. A.V.N. and N.S.W. contributed equally to this project.

Nutrient resorption and patterns of litter production and decomposition in a Neotropical savanna.

Treesearch

A.R. Kozovits; M.M.C. Bustamante; C.R. Garofalo; S. Bucci; A.C. Franco; G. Goldstein; F. Meinzer

2007-01-01

1. Deposition of nutrients owing to anthropogenic activities has the potential to change nutrient availability in nutrient-limited ecosystems with consequences for plant and ecosystem processes. 2. Species-specific and ecosystem responses to the addition of nutrients were studied in a field experiment conducted in a Savanna (Cerrado sensu stricto)...

Improving crop nutrient efficiency through root architecture modifications.

PubMed

Li, Xinxin; Zeng, Rensen; Liao, Hong

2016-03-01

Improving crop nutrient efficiency becomes an essential consideration for environmentally friendly and sustainable agriculture. Plant growth and development is dependent on 17 essential nutrient elements, among them, nitrogen (N) and phosphorus (P) are the two most important mineral nutrients. Hence it is not surprising that low N and/or low P availability in soils severely constrains crop growth and productivity, and thereby have become high priority targets for improving nutrient efficiency in crops. Root exploration largely determines the ability of plants to acquire mineral nutrients from soils. Therefore, root architecture, the 3-dimensional configuration of the plant's root system in the soil, is of great importance for improving crop nutrient efficiency. Furthermore, the symbiotic associations between host plants and arbuscular mycorrhiza fungi/rhizobial bacteria, are additional important strategies to enhance nutrient acquisition. In this review, we summarize the recent advances in the current understanding of crop species control of root architecture alterations in response to nutrient availability and root/microbe symbioses, through gene or QTL regulation, which results in enhanced nutrient acquisition. © 2015 Institute of Botany, Chinese Academy of Sciences.

[Changes of soil nutrient contents after prescribed burning of forestland in Heshan City, Guangdong Province].

PubMed

Sun, Yu-xin; Wu, Jian-ping; Zhou, Li-xia; Lin, Yong-biao; Fu, Sheng-lei

2009-03-01

A comparative study was conducted to analyze the changes of soil nutrient contents in Eucalyptus forestland and in shrubland after three years of prescribed burning. In Eucalyptus forestland, soil organic carbon, total nitrogen, available potassium contents and soil pH decreased significantly; soil available phosphorus and exchangeable magnesium contents, net nitrogen mineralization rate and ammonification rate also decreased but showed no significant difference. In shrubland, soil exchangeable calcium content increased significantly, but the contents of other nutrients had no significant change. The main reason of the lower soil net nitrogen mineralization rate in Eucalyptus forest could be the decrease of available substrates and the uptake of larger amount of soil nutrients by the fast growth of Eucalyptus. The soil nutrients in shrubland had a quick restoration rate after burning.

Does infection tilt the scales? Disease effects on the mass balance of an invertebrate nutrient recycler.

PubMed

Narr, Charlotte F; Frost, Paul C

2015-12-01

While parasites are increasingly recognized as important components of ecosystems, we currently know little about how they alter ecosystem nutrient availability via host-mediated nutrient cycling. We examined whether infection alters the flow of nutrients through hosts and whether such effects depend upon host diet quality. To do so, we compared the mass specific nutrient (i.e., nitrogen and phosphorus) release rates, ingestion rates, and elemental composition of uninfected Daphnia to those infected with a bacterial parasite, P. ramosa. N and P release rates were increased by infection when Daphnia were fed P-poor diets, but we found no effect of infection on the nutrient release of individuals fed P-rich diets. Calculations based on the first law of thermodynamics indicated that infection should increase the nutrient release rates of Daphnia by decreasing nutrient accumulation rates in host tissues. Although we found reduced nutrient accumulation rates in infected Daphnia fed all diets, this reduction did not increase the nutrient release rates of Daphnia fed the P-rich diet because infected Daphnia fed this diet ingested nutrients more slowly than uninfected hosts. Our results thus indicate that parasites can significantly alter the nutrient use of animal consumers, which could affect the availability of nutrients in heavily parasitized environments.

The convergence of psychology and neurobiology in flavor-nutrient learning.

PubMed

Myers, Kevin P

2018-03-01

Flavor evaluation is influenced by learning from experience with foods. One main influence is flavor-nutrient learning (FNL), a Pavlovian process whereby a flavor acts as a conditioned stimulus (CS) that becomes associated with the postingestive effects of ingested nutrients (the US). As a result that flavor becomes preferred and intake typically increases. This learning powerfully influences food choice and meal patterning. This paper summarizes how research elucidating the physiological and neural substrates of FNL has progressed in parallel with work characterizing how FNL affects perception, motivation, and behavior. The picture that emerges from this work is of a robust system of appetition (a term coined by Sclafani in contrast to the better-understood satiation signals) whereby ingested nutrients sensed in the gut evoke positive motivational responses. Appetition signals act within a meal to promote continued intake in immediate response to gut feedback, and act in the longer term to steer preference towards sensory cues that predict nutritional consequences. Copyright © 2017 Elsevier Ltd. All rights reserved.

Estimating photosynthesis with high resolution field spectroscopy in a Mediterranean grassland under different nutrient availability

NASA Astrophysics Data System (ADS)

Perez-Priego, O.; Guan, J.; Fava, F.; Rossini, M.; Wutzler, T.; Moreno, G.; Carrara, A.; Kolle, O.; Schrumpf, M.; Reichstein, M.; Migliavacca, M.

2014-12-01

Recent studies have shown how human induced N:P imbalances are affecting essential processes (e.g. photosynthesis, plant growth rate) that lead to important changes in ecosystem structure and function. In this regard, the accuracy of the approaches based on remotely-sensed data for monitoring and modeling gross primary production (GPP) relies on the ability of vegetation indices (VIs) to track the dynamics of vegetation physiological and biophysical properties/variables. Promising results have been recently obtained when Chlorophyll-sensitive VIs and Chlorophyll fluorescence are combined with structural indices in the framework of the Monteith's light use efficiency (LUE) model. However, further ground-based experiments are required to validate LUE model performances, and their capability to be generalized under different nutrient availability conditions. In this study, the overall objective was to investigate the sensitivity of VIs to track short- and long-term GPP variations in a Mediterranean grassland under different N and P fertilization treatments. Spectral VIs were acquired manually using high resolution spectrometers (HR4000, OceanOptics, USA) along a phenological cycle. The VIs examined included photochemical reflectance index (PRI), MERIS terrestrial-chlorophyll index (MTCI) and normalized difference vegetation index (NDVI). Solar-induced chlorophyll fluorescence calculated at the oxygen absorption band O2-A (F760) using spectral fitting methods was also used. Simultaneously, measurements of GPP and environmental variables were conducted using a transient-state canopy chamber. Overall, GPP, F760 and VIs showed a clear seasonal time-trend in all treatments, which was driven by the phenological development of the grassland. Results showed significant differences (p<0.05) in midday GPP values between N and without N addition plots, in particular at the peak of the growing season during the flowering stage and at the end of the season during senescence. While NDVI did not show any significant difference between treatments, VIs sensitive to pigment variations and physiology (PRI, MTCI) and F760 behaved as GPP. Model performance test indicated that VIs related to physiology and fluorescence are key to account for nutrient availability in LUE models and to better predict GPP.

Dynamics of submarine groundwater discharge and associated fluxes of dissolved nutrients, carbon, and trace gases to the coastal zone (Okatee River estuary, South Carolina)

USGS Publications Warehouse

Porubsky, W.P.; Weston, N.B.; Moore, W.S.; Ruppel, C.; Joye, S.B.

2014-01-01

Multiple techniques, including thermal infrared aerial remote sensing, geophysical and geological data, geochemical characterization and radium isotopes, were used to evaluate the role of groundwater as a source of dissolved nutrients, carbon, and trace gases to the Okatee River estuary, South Carolina. Thermal infrared aerial remote sensing surveys illustrated the presence of multiple submarine groundwater discharge sites in Okatee headwaters. Significant relationships were observed between groundwater geochemical constituents and 226Ra activity in groundwater with higher 226Ra activity correlated to higher concentrations of organics, dissolved inorganic carbon, nutrients, and trace gases to the Okatee system. A system-level radium mass balance confirmed a substantial submarine groundwater discharge contribution of these constituents to the Okatee River. Diffusive benthic flux measurements and potential denitrification rate assays tracked the fate of constituents in creek bank sediments. Diffusive benthic fluxes were substantially lower than calculated radium-based submarine groundwater discharge inputs, showing that advection of groundwater-derived nutrients dominated fluxes in the system. While a considerable potential for denitrification in tidal creek bank sediments was noted, in situ denitrification rates were nitrate-limited, making intertidal sediments an inefficient nitrogen sink in this system. Groundwater geochemical data indicated significant differences in groundwater chemical composition and radium activity ratios between the eastern and western sides of the river; these likely arose from the distinct hydrological regimes observed in each area. Groundwater from the western side of the Okatee headwaters was characterized by higher concentrations of dissolved organic and inorganic carbon, dissolved organic nitrogen, inorganic nutrients and reduced metabolites and trace gases, i.e. methane and nitrous oxide, than groundwater from the eastern side. Differences in microbial sulfate reduction, organic matter supply, and/or groundwater residence time likely contributed to this pattern. The contrasting features of the east and west sub-marsh zones highlight the need for multiple techniques for characterization of submarine groundwater discharge sources and the impact of biogeochemical processes on the delivery of nutrients and carbon to coastal areas via submarine groundwater discharge.

Adaptive cover crop implementation and evaluation in the Chesapeake Bay watersheds

USDA-ARS?s Scientific Manuscript database

Four years of applied research, led by the USDA-ARS Choptank River Conservation Reserve Assessment Project (CEAP), have demonstrated that satellite remote sensing, used in combination with cost-share program farm implementation records, can provide a powerful tool for evaluating the nutrient conserv...

Uncoupling the complexity of forest soil variation: influence of terrain attributes, spectral indices, and spatial variability

EPA Science Inventory

Growing concern over climate and management induced changes to soil nutrient status has prompted interest in understanding the spatial distribution of forest soil properties. Recent advancements in remotely sensed geospatial technologies are providing an increasing array of data...

Nutrient availability in rangeland soils: influence of prescribed burning, herbaceous vegetation removal, overseeding with Bromus tectorum, season, and elevation

Treesearch

R. R. Blank; J. Chambers; B. Roundy; A. Whittaker

2007-01-01

Soil nutrient availability influences plant invasions. Resin capsules were used to examine soil nutrient bioavailability along 2 sagebrush-grassland elevation transects in the east Tintic Range (Utah) and Shoshone Range (Nevada). In the fall of 2001, treatments were applied to 3 replicate plots at each site, which included prescribed burning, herbaceous vegetation...

Machine processing of remotely sensed data - quantifying global process: Models, sensor systems, and analytical methods; Proceedings of the Eleventh International Symposium, Purdue University, West Lafayette, IN, June 25-27, 1985

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mengel, S.K.; Morrison, D.B.

1985-01-01

Consideration is given to global biogeochemical issues, image processing, remote sensing of tropical environments, global processes, geology, landcover hydrology, and ecosystems modeling. Topics discussed include multisensor remote sensing strategies, geographic information systems, radars, and agricultural remote sensing. Papers are presented on fast feature extraction; a computational approach for adjusting TM imagery terrain distortions; the segmentation of a textured image by a maximum likelihood classifier; analysis of MSS Landsat data; sun angle and background effects on spectral response of simulated forest canopies; an integrated approach for vegetation/landcover mapping with digital Landsat images; geological and geomorphological studies using an image processing technique;more » and wavelength intensity indices in relation to tree conditions and leaf-nutrient content.« less

Controls of bedrock geochemistry on soil and plant nutrients in Southeastern Utah

USGS Publications Warehouse

Neff, J.C.; Reynolds, R.; Sanford, R.L.; Fernandez, D.; Lamothe, P.

2006-01-01

The cold deserts of the Colorado Plateau contain numerous geologically and geochemically distinct sedimentary bedrock types. In the area near Canyonlands National Park in Southeastern Utah, geochemical variation in geologic substrates is related to the depositional environment with higher concentrations of Fe, Al, P, K, and Mg in sediments deposited in alluvial or marine environments and lower concentrations in bedrock derived from eolian sand dunes. Availability of soil nutrients to vegetation is also controlled by the formation of secondary minerals, particularly for P and Ca availability, which, in some geologic settings, appears closely related to variation of CaCO3 and Ca-phosphates in soils. However, the results of this study also indicate that P content is related to bedrock and soil Fe and Al content suggesting that the deposition history of the bedrock and the presence of P-bearing Fe and Al minerals, is important to contemporary P cycling in this region. The relation between bedrock type and exchangeable Mg and K is less clear-cut, despite large variation in bedrock concentrations of these elements. We examined soil nutrient concentrations and foliar nutrient concentration of grasses, shrubs, conifers, and forbs in four geochemically distinct field sites. All four of the functional plant groups had similar proportional responses to variation in soil nutrient availability despite large absolute differences in foliar nutrient concentrations and stoichiometry across species. Foliar P concentration (normalized to N) in particular showed relatively small variation across different geochemical settings despite large variation in soil P availability in these study sites. The limited foliar variation in bedrock-derived nutrients suggests that the dominant plant species in this dryland setting have a remarkably strong capacity to maintain foliar chemistry ratios despite large underlying differences in soil nutrient availability. ?? 2006 Springer Science+Business Media, Inc.

Phosphate addition enhanced soil inorganic nutrients to a large extent in three tropical forests.

PubMed

Zhu, Feifei; Lu, Xiankai; Liu, Lei; Mo, Jiangming

2015-01-21

Elevated nitrogen (N) deposition may constrain soil phosphorus (P) and base cation availability in tropical forests, for which limited evidence have yet been available. In this study, we reported responses of soil inorganic nutrients to full factorial N and P treatments in three tropical forests different in initial soil N status (N-saturated old-growth forest and two less-N-rich younger forests). Responses of microbial biomass, annual litterfall production and nutrient input were also monitored. Results showed that N treatments decreased soil inorganic nutrients (except N) in all three forests, but the underlying mechanisms varied depending on forests: through inhibition on litter decomposition in the old-growth forest and through Al(3+) replacement of Ca(2+) in the two younger forests. In contrast, besides great elevation in soil available P, P treatments induced 60%, 50%, 26% increases in sum of exchangeable (K(+)+Ca(2+)+Mg(2+)) in the old-growth and the two younger forests, respectively. These positive effects of P were closely related to P-stimulated microbial biomass and litter nutrient input, implying possible stimulation of nutrient return. Our results suggest that N deposition may result in decreases in soil inorganic nutrients (except N) and that P addition can enhance soil inorganic nutrients to support ecosystem processes in these tropical forests.

Phosphate addition enhanced soil inorganic nutrients to a large extent in three tropical forests

PubMed Central

Zhu, Feifei; Lu, Xiankai; Liu, Lei; Mo, Jiangming

2015-01-01

Elevated nitrogen (N) deposition may constrain soil phosphorus (P) and base cation availability in tropical forests, for which limited evidence have yet been available. In this study, we reported responses of soil inorganic nutrients to full factorial N and P treatments in three tropical forests different in initial soil N status (N-saturated old-growth forest and two less-N-rich younger forests). Responses of microbial biomass, annual litterfall production and nutrient input were also monitored. Results showed that N treatments decreased soil inorganic nutrients (except N) in all three forests, but the underlying mechanisms varied depending on forests: through inhibition on litter decomposition in the old-growth forest and through Al3+ replacement of Ca2+ in the two younger forests. In contrast, besides great elevation in soil available P, P treatments induced 60%, 50%, 26% increases in sum of exchangeable (K++Ca2++Mg2+) in the old-growth and the two younger forests, respectively. These positive effects of P were closely related to P-stimulated microbial biomass and litter nutrient input, implying possible stimulation of nutrient return. Our results suggest that N deposition may result in decreases in soil inorganic nutrients (except N) and that P addition can enhance soil inorganic nutrients to support ecosystem processes in these tropical forests. PMID:25605567

Major role of nutrient supply in the control of picophytoplankton community structure

NASA Astrophysics Data System (ADS)

Mouriño, B.; Agusti, S.; Bode, A.; Cermeno, P.; Chouciño, P.; da Silva, J. C. B.; Fernández-Castro, B.; Gasol, J.; Gil Coto, M.; Graña, R.; Latasa, M.; Lubián, L.; Marañón, E.; Moran, X. A.; Moreno, E.; Moreira-Coello, V.; Otero-Ferrer, J. L.; Ruiz Villarreal, M.; Scharek, R.; Vallina, S. M.; Varela, M.; Villamaña, M.

2016-02-01

The Margalef's mandala (1978) is a simplified bottom-up control model that explains how mixing and nutrient concentration determine the composition of marine phytoplankton communities. Due to the difficulties of measuring turbulence in the field, previous attempts to verify this model have applied different proxies for nutrient supply, and very often used interchangeably the terms mixing and stratification. Moreover, because the mandala was conceived before the discovery of smaller phytoplankton groups (picoplankton <2 μm), it describes only the succession of vegetative phases of microplankton. In order to test the applicability of the classical mandala to picoplankton groups, we used a multidisciplinary approach including specifically designed field observations supported by remote sensing, database analyses, and modeling and laboratory chemostat experiments. Simultaneous estimates of nitrate diffusive fluxes, derived from microturbulence observations, and picoplankton abundance collected in more than 200 stations, spanning widely different hydrographic regimes, showed that the contribution of eukaryotes to picoautotrophic biomass increases with nutrient supply, whereas that of picocyanobacteria shows the opposite trend. These findings were supported by laboratory and modeling chemostat experiments that reproduced the competitive dynamics between picoeukaryote sand picocyanobacteria as a function of changing nutrient supply. Our results indicate that nutrient supply controls the distribution of picoplankton functional groups in the ocean, further supporting the model proposed by Margalef.

Understanding complexities in coupled dynamics of human-water and food security

NASA Astrophysics Data System (ADS)

Usmani, M.; Kondal, A.; Lin, L.; Colwell, R. R.; Jutla, A.

2017-12-01

Traditional premise of food security is associated with satisfying human hunger by providing sufficient calories to population. Water is the key variable associated with the growth of crops, which is then used as a metric of success for abundance of food across globe. The current framework often negates complex coupled interaction between availability of food nutrients and human well-being (such as productivity, work efficiency, low birth weight, physical and mental growth). Our analysis suggests that 1 in 3 humans suffer from malnutrition across the globe. In last five decades, most of the countries have a decreasing availability trend in at least one of the twenty-three essential food nutrients required for human well-being. We argue that food security can only be achieved if information on use of water for crops and consumption of food must include availability of nutrients for humans. Here, we propose a new concept of "consumptive nutrients" that include constant feedback mechanism between water-human and societal processes- essential for growth, distribution and consumption of food nutrients. Using Ethiopia as a signature rain-fed agricultural region, we will show how decreasing precipitation has led to an increase in crop productivity, but decreased availability of nutrients for humans. This in turn has destabilizing impact on overall regional economy. We will demonstrate why inclusion of nutrients must be a part of discussion for ensuring food security to human population.

Breeding crops for improved mineral nutrition under climate change conditions.

PubMed

Pilbeam, David J

2015-06-01

Improvements in understanding how climate change may influence chemical and physical processes in soils, how this may affect nutrient availability, and how plants may respond to changed availability of nutrients will influence crop breeding programmes. The effects of increased atmospheric CO2 and warmer temperatures, both individually and combined, on soil microbial activity, including mycorrhizas and N-fixing organisms, are evaluated, together with their implications for nutrient availability. Potential changes to plant growth, and the combined effects of soil and plant changes on nutrient uptake, are discussed. The organization of research on the efficient use of macro- and micronutrients by crops under climate change conditions is outlined, including analysis of QTLs for nutrient efficiency. Suggestions for how the information gained can be used in plant breeding programmes are given. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Effects of soil nutrient heterogeneity on intraspecific competition in the invasive, clonal plant Alternanthera philoxeroides.

PubMed

Zhou, Jian; Dong, Bi-Cheng; Alpert, Peter; Li, Hong-Li; Zhang, Ming-Xiang; Lei, Guang-Chun; Yu, Fei-Hai

2012-03-01

Fine-scale, spatial heterogeneity in soil nutrient availability can increase the growth of individual plants, the productivity of plant communities and interspecific competition. If this is due to the ability of plants to concentrate their roots where nutrient levels are high, then nutrient heterogeneity should have little effect on intraspecific competition, especially when there are no genotypic differences between individuals in root plasticity. We tested this hypothesis in a widespread, clonal species in which individual plants are known to respond to nutrient heterogeneity. Plants derived from a single clone of Alternanthera philoxeroides were grown in the greenhouse at low or high density (four or 16 plants per 27·5 × 27·5-cm container) with homogeneous or heterogeneous availability of soil nutrients, keeping total nutrient availability per container constant. After 9 weeks, measurements of size, dry mass and morphology were taken. Plants grew more in the heterogeneous than in the homogeneous treatment, showing that heterogeneity promoted performance; they grew less in the high- than in the low-density treatment, showing that plants competed. There was no interactive effect of nutrient heterogeneity and plant density, supporting the hypothesis that heterogeneity does not affect intraspecific competition in the absence of genotypic differences in plasticity. Treatments did not affect morphological characteristics such as specific leaf area or root/shoot ratio. Results indicate that fine-scale, spatial heterogeneity in the availability of soil nutrients does not increase competition when plants are genetically identical, consistent with the suggestion that effects of heterogeneity on competition depend upon differences in plasticity between individuals. Heterogeneity is only likely to increase the spread of monoclonal, invasive populations such as that of A. philoxeroides in China.

Nitrogen regulation of transpiration controls mass-flow acquisition of nutrients.

PubMed

Matimati, Ignatious; Verboom, G Anthony; Cramer, Michael D

2014-01-01

Transpiration may enhance mass-flow of nutrients to roots, especially in low-nutrient soils or where the root system is not extensively developed. Previous work suggested that nitrogen (N) may regulate mass-flow of nutrients. Experiments were conducted to determine whether N regulates water fluxes, and whether this regulation has a functional role in controlling the mass-flow of nutrients to roots. Phaseolus vulgaris were grown in troughs designed to create an N availability gradient by restricting roots from intercepting a slow-release N source, which was placed at one of six distances behind a 25 μm mesh from which nutrients could move by diffusion or mass-flow (termed 'mass-flow' treatment). Control plants had the N source supplied directly to their root zone so that N was available through interception, mass-flow, and diffusion (termed 'interception' treatment). 'Mass-flow' plants closest to the N source exhibited 2.9-fold higher transpiration (E), 2.6-fold higher stomatal conductance (gs), 1.2-fold higher intercellular [CO2] (Ci), and 3.4-fold lower water use efficiency than 'interception' plants, despite comparable values of photosynthetic rate (A). E, gs, and Ci first increased and then decreased with increasing distance from the N source to values even lower than those of 'interception' plants. 'Mass-flow' plants accumulated phosphorus and potassium, and had maximum concentrations at 10mm from the N source. Overall, N availability regulated transpiration-driven mass-flow of nutrients from substrate zones that were inaccessible to roots. Thus when water is available, mass-flow may partially substitute for root density in providing access to nutrients without incurring the costs of root extension, although the efficacy of mass-flow also depends on soil nutrient retention and hydraulic properties.

Nitrogen regulation of transpiration controls mass-flow acquisition of nutrients

PubMed Central

Matimati, Ignatious

2014-01-01

Transpiration may enhance mass-flow of nutrients to roots, especially in low-nutrient soils or where the root system is not extensively developed. Previous work suggested that nitrogen (N) may regulate mass-flow of nutrients. Experiments were conducted to determine whether N regulates water fluxes, and whether this regulation has a functional role in controlling the mass-flow of nutrients to roots. Phaseolus vulgaris were grown in troughs designed to create an N availability gradient by restricting roots from intercepting a slow-release N source, which was placed at one of six distances behind a 25 μm mesh from which nutrients could move by diffusion or mass-flow (termed ‘mass-flow’ treatment). Control plants had the N source supplied directly to their root zone so that N was available through interception, mass-flow, and diffusion (termed ‘interception’ treatment). ‘Mass-flow’ plants closest to the N source exhibited 2.9-fold higher transpiration (E), 2.6-fold higher stomatal conductance (g s), 1.2-fold higher intercellular [CO2] (C i), and 3.4-fold lower water use efficiency than ‘interception’ plants, despite comparable values of photosynthetic rate (A). E, g s, and C i first increased and then decreased with increasing distance from the N source to values even lower than those of ‘interception’ plants. ‘Mass-flow’ plants accumulated phosphorus and potassium, and had maximum concentrations at 10mm from the N source. Overall, N availability regulated transpiration-driven mass-flow of nutrients from substrate zones that were inaccessible to roots. Thus when water is available, mass-flow may partially substitute for root density in providing access to nutrients without incurring the costs of root extension, although the efficacy of mass-flow also depends on soil nutrient retention and hydraulic properties. PMID:24231035

Coupling nutrient sensing to metabolic homoeostasis: the role of the mammalian target of rapamycin complex 1 pathway.

PubMed

André, Caroline; Cota, Daniela

2012-11-01

The mammalian target of rapamycin complex 1 (mTORC1) pathway is known to couple different environmental cues to the regulation of several energy-demanding functions within the cell, spanning from protein translation to mitochondrial activity. As a result, at the organism level, mTORC1 activity affects energy balance and general metabolic homoeostasis by modulating both the activity of neuronal populations that play key roles in the control of food intake and body weight, as well as by determining storage and use of fuel substrates in peripheral tissues. This review focuses on recent advances made in understanding the role of the mTORC1 pathway in the regulation of energy balance. More particularly, it aims at providing an overview of the status of knowledge regarding the mechanisms underlying the ability of certain amino acids, glucose and fatty acids, to affect mTORC1 activity and in turn illustrates how the mTORC1 pathway couples nutrient sensing to the hypothalamic regulation of the organisms' energy homoeostasis and to the control of intracellular metabolic processes, such as glucose uptake, protein and lipid biosynthesis. The evidence reviewed pinpoints the mTORC1 pathway as an integrator of the actions of nutrients on metabolic health and provides insight into the relevance of this intracellular pathway as a potential target for the therapy of metabolic diseases such as obesity and type-2 diabetes.

Comparative Study on the Cellular and Systemic Nutrient Sensing and Intermediary Metabolism after Partial Replacement of Fishmeal by Meat and Bone Meal in the Diet of Turbot (Scophthalmus maximus L.)

PubMed Central

Mai, Kangsen; Zhou, Huihui; Xu, Wei; He, Gen

2016-01-01

This study was designed to examine the cellular and systemic nutrient sensing mechanisms as well as the intermediary metabolism responses in turbot (Scophthalmus maximus L.) fed with fishmeal diet (FM diet), 45% of FM replaced by meat and bone meal diet (MBM diet) or MBM diet supplemented with essential amino acids to match the amino acid profile of FM diet (MBM+AA diet). During the one month feeding trial, feed intake was not affected by the different diets. However, MBM diet caused significant reduction of specific growth rate and nutrient retentions. Compared with the FM diet, MBM diet down-regulated target of rapamycin (TOR) and insulin-like growth factor (IGFs) signaling pathways, whereas up-regulated the amino acid response (AAR) signaling pathway. Moreover, MBM diet significantly decreased glucose and lipid anabolism, while increased muscle protein degradation and lipid catabolism in liver. MBM+AA diet had no effects on improvement of MBM diet deficiencies. Compared with fasted, re-feeding markedly activated the TOR signaling pathway, IGF signaling pathway and glucose, lipid metabolism, while significantly depressed the protein degradation signaling pathway. These results thus provided a comprehensive display of molecular responses and a better explanation of deficiencies generated after fishmeal replacement by other protein sources. PMID:27802317

Complex physiological and molecular processes underlying root gravitropism

NASA Technical Reports Server (NTRS)

Chen, Rujin; Guan, Changhui; Boonsirichai, Kanokporn; Masson, Patrick H.

2002-01-01

Gravitropism allows plant organs to guide their growth in relation to the gravity vector. For most roots, this response to gravity allows downward growth into soil where water and nutrients are available for plant growth and development. The primary site for gravity sensing in roots includes the root cap and appears to involve the sedimentation of amyloplasts within the columella cells. This process triggers a signal transduction pathway that promotes both an acidification of the wall around the columella cells, an alkalinization of the columella cytoplasm, and the development of a lateral polarity across the root cap that allows for the establishment of a lateral auxin gradient. This gradient is then transmitted to the elongation zones where it triggers a differential cellular elongation on opposite flanks of the central elongation zone, responsible for part of the gravitropic curvature. Recent findings also suggest the involvement of a secondary site/mechanism of gravity sensing for gravitropism in roots, and the possibility that the early phases of graviresponse, which involve differential elongation on opposite flanks of the distal elongation zone, might be independent of this auxin gradient. This review discusses our current understanding of the molecular and physiological mechanisms underlying these various phases of the gravitropic response in roots.

Metabolic flexibility as an adaptation to energy resources and requirements in health and disease.

PubMed

Smith, Reuben L; Soeters, Maarten R; Wüst, Rob C I; Houtkooper, Riekelt H

2018-04-24

The ability to efficiently adapt metabolism by substrate sensing, trafficking, storage and utilization, dependent on availability and requirement is known as metabolic flexibility. In this review, we discuss the breadth and depth of metabolic flexibility and its impact on health and disease. Metabolic flexibility is essential to maintain energy homeostasis in times of either caloric excess or caloric restriction, and in times of either low or high energy demand, such as during exercise. The liver, adipose tissue and muscle govern systemic metabolic flexibility and manage nutrient sensing, uptake, transport, storage and expenditure by communication via endocrine cues. At a molecular level, metabolic flexibility relies on the configuration of metabolic pathways which is regulated by key metabolic enzymes and transcription factors, many of which interact closely with the mitochondria. Disrupted metabolic flexibility, or metabolic inflexibility, however, is associated with many pathological conditions including metabolic syndrome, type 2 diabetes mellitus, and cancer. Multiple factors like dietary composition and feeding frequency, exercise training, and use of pharmacological compounds influence metabolic flexibility and will be discussed here. Lastly, we outline important advances in metabolic flexibility research and discuss medical horizons and translational aspects.

A nutrient dependant switch explains mutually exclusive existence of meiosis and mitosis initiation in budding yeast.

PubMed

Wannige, C T; Kulasiri, D; Samarasinghe, S

2014-01-21

Nutrients from living environment are vital for the survival and growth of any organism. Budding yeast diploid cells decide to grow by mitosis type cell division or decide to create unique, stress resistant spores by meiosis type cell division depending on the available nutrient conditions. To gain a molecular systems level understanding of the nutrient dependant switching between meiosis and mitosis initiation in diploid cells of budding yeast, we develop a theoretical model based on ordinary differential equations (ODEs) including the mitosis initiator and its relations to budding yeast meiosis initiation network. Our model accurately and qualitatively predicts the experimentally revealed temporal variations of related proteins under different nutrient conditions as well as the diverse mutant studies related to meiosis and mitosis initiation. Using this model, we show how the meiosis and mitosis initiators form an all-or-none type bistable switch in response to available nutrient level (mainly nitrogen). The transitions to and from meiosis or mitosis initiation states occur via saddle node bifurcation. This bidirectional switch helps the optimal usage of available nutrients and explains the mutually exclusive existence of meiosis and mitosis pathways. © 2013 Elsevier Ltd. All rights reserved.

Remote sensing to monitor cover crop adoption in southeastern Pennsylvania

USDA-ARS?s Scientific Manuscript database

In the Chesapeake Bay watershed, winter cereal cover crops are often planted in rotation with summer crops to reduce the loss of nutrients and sediment from agricultural systems. Cover crops can also improve soil health, control weeds and pests, supplement forage needs, and support resilient croppin...

Laboratory Evaluation of Ion-Selective Electrodes for Simultaneous Analysis of Macronutrients in Hydroponic Solution

USDA-ARS?s Scientific Manuscript database

Automated sensing of macronutrients in hydroponic solution would allow more efficient management of nutrients for crop growth in closed hydroponic systems. Ion-selective microelectrode technology requires an ion-selective membrane or a solid metal material that responds selectively to one analyte in...

Shotgun proteomic analysis of Yersinia ruckeri isolates under normal and iron-limited conditions

USDA-ARS?s Scientific Manuscript database

Yersinia ruckeri is the causative agent of enteric redmouth disease of fish and causes significant economic losses, particularly in salmonids. Iron is an essential nutrient for many cellular processes and is involved in host sensing and virulence regulation in many bacteria. Bacterial pathogens diff...

HIGH SPATIAL RESOLUTION SATELLITE REMOTE SENSING FOR PLANNING AND LOCATING ANIMAL FEEDING OPERATIONS

EPA Science Inventory

Surface runoff of animal waste and its infiltration into groundwater can pose a number of risks to water quality mainly because of the amount of animal manure and wastewater they produce. Excess nutrients from livestock facilities can lead to groundwater and soil contaminatio...

Extracellular enzymes in sensing environmental nutrients and ecosystem changes: Ligand mediation in organic phosphorus cycling

USDA-ARS?s Scientific Manuscript database

Inorganic and organic phosphates react strongly with soil constituents, resulting in relatively low concentrations of soluble P in the soil solution. Multiple competing reactions are operating to regulate the solution-phase concentration of P-containing organic substrates and the released phosphate...

Does encouraging the use of wetlands in water quality trading Programs make economic sense? presentation

EPA Science Inventory

Water quality trading has grown in popularity and scope in recent years owing to its potential as a flexible, low-cost way to achieve nutrient reduction goals. Policy makers are interested in encouraging traders, primarily agricultural sources, to use restored and constructed we...

Remote sensing of perennial crop stand duration and pre-crop identification

USDA-ARS?s Scientific Manuscript database

Field to field variability in soil erosion and off-site transport of nutrients and pesticides in western Oregon in any single year is primarily driven by the question of whether individual fields were disturbed for planting of new crop stands or remained in production of established perennial crops...

Effects of fire alone or combined with thinning on tissue nutrient concentrations and nutrient resorption in Desmodium nudiflorum.

PubMed

Huang, Jianjun; Boerner, Ralph E J

2007-08-01

This study examined tissue nutrient responses of Desmodium nudiflorum to changes in soil total inorganic nitrogen (TIN) and available phosphorus (P) that occurred as the result of the application of alternative forest management strategies, namely (1) prescribed low-intensity fire (B), (2) overstory thinning followed by prescribed fire (T + B), and (3) untreated control C), in two Quercus-dominated forests in the State of Ohio, USA. In the fourth growing season after a first fire, TIN was significantly greater in the control plots (9.8 mg/kg) than in the B (5.5 mg/kg) and T + B (6.4 mg/kg) plots. Similarly, available P was greater in the control sites (101 microg/g) than in the B (45 microg/kg) and T + B (65 microg/kg) sites. Leaf phosphorus ([P]) was higher in the plants from control site (1.86 mg/g) than in either the B (1.77 mg/g) or T + B plants (1.73 mg/g). Leaf nitrogen ([N]) and root [N] showed significant site-treatment interactive effects, while stem [N], stem [P], and root [P] did not differ significantly among treatments. During the first growing season after a second fire, leaf [N], stem [N], litter [P] and available soil [P] were consistently lower in plots of the manipulated treatments than in the unmanaged control plot, whereas the B and T + B plots did not differ significantly from each other. N resorption efficiency was positively correlated with the initial foliar [N] in the manipulated (B and T + B) sites, but there was no such relation in the unmanaged control plots. P resorption efficiency was positively correlated with the initial leaf [P] in both the control and manipulated plots. Leaf nutrient status was strongly influenced by soil nutrient availability shortly after fire, but became more influenced by topographic position in the fourth year after fire. Nutrient resorption efficiency was independent of soil nutrient availability. These findings enrich our understanding of the effects of ecosystem restoration treatments on soil nutrient availability, plant nutrient relations, and plant-soil interactions at different temporal scales.

A Comparison of the Role of Episode Nutrient Supply on Pathways of Carbon in Upwelling Regimes

NASA Technical Reports Server (NTRS)

Carr, M. E.

1997-01-01

Nutrient supply is episode in the ocean even in regions of fairly high and continuous nutrient supply, such as coastal upwelling regimes. The structure of the ecosystem depends on nutrient availability and the different requirements of phytoplankton cells.

Development of a Self-calibrating Dissolved Oxygen Microsensor Array for the Monitoring and Control of Plant Growth in a Space Environment

NASA Technical Reports Server (NTRS)

Kim, Chang-Soo; Brown, Christopher S.; Nagle, H. Troy

2004-01-01

Plant experiments in space will require active nutrient delivery concepts in which water and nutrients are replenished on a continuous basis for long-term growth. The goal of this study is to develop a novel microsensor array to provide information on the dissolved oxygen environment in the plant root zone for the optimum control of plant cultivation systems in the space environment. Control of water and oxygen is limited by the current state-of-the-art in sensor technology. Two capabilities of the new microsensor array were tested. First, a novel in situ self-diagnosis/self-calibration capability for the microsensor was explored by dynamically controlling the oxygen microenvironment in close proximity to an amperometric dissolved oxygen microsensors. A pair of integrated electrochemical actuator electrodes provided the microenvironments based on water electrolysis. Miniaturized thin film dissolved oxygen microsensors on a flexible polyimide (Kapton(Registered Trademark)? substrate were fabricated and their performances were tested. Secondly, measurements of dissolved oxygen in two representative plant growth systems were made, which had not been performed previously due to lack of proper sensing technology. The responses of the oxygen microsensor array on a flexible polymer substrate properly reflected the oxygen contents on the surface of a porous tube nutrient delivery system and within a particulate substrate system. Additionally, we demonstrated the feasibility of using a 4-point thin film microprobe for water contents measurements for both plant growth systems. mechanical flexibility, and self-diagnosis. The proposed technology is anticipated to provide a reliable sensor feedback plant growth nutrient delivery systems in both terrestrial environment and the microgravity environment during long term space missions. The unique features of the sensor include small size and volume, multiple-point sensing,

pH homeostasis links the nutrient sensing PKA/TORC1/Sch9 ménage-à-trois to stress tolerance and longevity.

PubMed

Deprez, Marie-Anne; Eskes, Elja; Wilms, Tobias; Ludovico, Paula; Winderickx, Joris

2018-01-12

The plasma membrane H + -ATPase Pma1 and the vacuolar V-ATPase act in close harmony to tightly control pH homeostasis, which is essential for a vast number of physiological processes. As these main two regulators of pH are responsive to the nutritional status of the cell, it seems evident that pH homeostasis acts in conjunction with nutrient-induced signalling pathways. Indeed, both PKA and the TORC1-Sch9 axis influence the proton pumping activity of the V-ATPase and possibly also of Pma1. In addition, it recently became clear that the proton acts as a second messenger to signal glucose availability via the V-ATPase to PKA and TORC1-Sch9. Given the prominent role of nutrient signalling in longevity, it is not surprising that pH homeostasis has been linked to ageing and longevity as well. A first indication is provided by acetic acid, whose uptake by the cell induces toxicity and affects longevity. Secondly, vacuolar acidity has been linked to autophagic processes, including mitophagy. In agreement with this, a decline in vacuolar acidity was shown to induce mitochondrial dysfunction and shorten lifespan. In addition, the asymmetric inheritance of Pma1 has been associated with replicative ageing and this again links to repercussions on vacuolar pH. Taken together, accumulating evidence indicates that pH homeostasis plays a prominent role in the determination of ageing and longevity, thereby providing new perspectives and avenues to explore the underlying molecular mechanisms.

Biogeochemical cycling in terrestrial ecosystems - Modeling, measurement, and remote sensing

NASA Technical Reports Server (NTRS)

Peterson, D. L.; Matson, P. A.; Lawless, J. G.; Aber, J. D.; Vitousek, P. M.

1985-01-01

The use of modeling, remote sensing, and measurements to characterize the pathways and to measure the rate of biogeochemical cycling in forest ecosystems is described. The application of the process-level model to predict processes in intact forests and ecosystems response to disturbance is examined. The selection of research areas from contrasting climate regimes and sites having a fertility gradient in that regime is discussed, and the sites studied are listed. The use of remote sensing in determining leaf area index and canopy biochemistry is analyzed. Nitrous oxide emission is investigated by using a gas measurement instrument. Future research projects, which include studying the influence of changes on nutrient cycling in ecosystems and the effect of pollutants on the ecosystems, are discussed.

[An optical-fiber-sensor-based spectrophotometer for soil non-metallic nutrient determination].

PubMed

He, Dong-xian; Hu, Juan-xiu; Lu, Shao-kun; He, Hou-yong

2012-01-01

In order to achieve rapid, convenient and efficient soil nutrient determination in soil testing and fertilizer recommendation, a portable optical-fiber-sensor-based spectrophotometer including immersed fiber sensor, flat field holographic concave grating, and diode array detector was developed for soil non-metallic nutrient determination. According to national standard of ultraviolet and visible spectrophotometer with JJG 178-2007, the wavelength accuracy and repeatability, baseline stability, transmittance accuracy and repeatability measured by the prototype instrument were satisfied with the national standard of III level; minimum spectral bandwidth, noise and excursion, and stray light were satisfied with the national standard of IV level. Significant linear relationships with slope of closing to 1 were found between the soil available nutrient contents including soil nitrate nitrogen, ammonia nitrogen, available phosphorus, available sulfur, available boron, and organic matter measured by the prototype instrument compared with that measured by two commercial single-beam-based and dual-beam-based spectrophotometers. No significant differences were revealed from the above comparison data. Therefore, the optical-fiber-sensor-based spectrophotometer can be used for rapid soil non-metallic nutrient determination with a high accuracy.

Water: neglected, unappreciated and under researched.

PubMed

Rush, E C

2013-05-01

Water, an essential nutrient, is often ignored in reports of dietary surveys and nutrition. Although it is ubiquitous in foods and beverages, the attention is often focused on the minerals or calorific values of the fluids imbibed rather than the water per se. Water is often taken for granted by many in Western countries due to its abundant availability through water systems. In developing countries, however, water and sanitation raise significant problems. This review overviews (i) the global perspective of the potable water supply, (ii) human rights and water, (iii) dietary guidelines and sources of water and (iv) the physiology of water balance. Gaps in knowledge and understanding around hydration and water requirements are also discussed. Nutritionists are urged to look at the bigger picture of the global water supply and to use good judgement and common sense when advising on water requirements.

IKKβ promotes metabolic adaptation to glutamine deprivation via phosphorylation and inhibition of PFKFB3

PubMed Central

Reid, Michael A.; Lowman, Xazmin H.; Pan, Min; Tran, Thai Q.; Warmoes, Marc O.; Ishak Gabra, Mari B.; Yang, Ying; Locasale, Jason W.; Kong, Mei

2016-01-01

Glutamine is an essential nutrient for cancer cell survival and proliferation. Enhanced utilization of glutamine often depletes its local supply, yet how cancer cells adapt to low glutamine conditions is largely unknown. Here, we report that IκB kinase β (IKKβ) is activated upon glutamine deprivation and is required for cell survival independently of NF-κB transcription. We demonstrate that IKKβ directly interacts with and phosphorylates 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase isoform 3 (PFKFB3), a major driver of aerobic glycolysis, at Ser269 upon glutamine deprivation to inhibit its activity, thereby down-regulating aerobic glycolysis when glutamine levels are low. Thus, due to lack of inhibition of PFKFB3, IKKβ-deficient cells exhibit elevated aerobic glycolysis and lactate production, leading to less glucose carbons contributing to tricarboxylic acid (TCA) cycle intermediates and the pentose phosphate pathway, which results in increased glutamine dependence for both TCA cycle intermediates and reactive oxygen species suppression. Therefore, coinhibition of IKKβ and glutamine metabolism results in dramatic synergistic killing of cancer cells both in vitro and in vivo. In all, our results uncover a previously unidentified role of IKKβ in regulating glycolysis, sensing low-glutamine-induced metabolic stress, and promoting cellular adaptation to nutrient availability. PMID:27585591

Deciphering the metabolic response of M ycobacterium tuberculosis to nitrogen stress

PubMed Central

Williams, Kerstin J.; Jenkins, Victoria A.; Barton, Geraint R.; Bryant, William A.; Krishnan, Nitya

2015-01-01

Summary A key component to the success of M ycobacterium tuberculosis as a pathogen is the ability to sense and adapt metabolically to the diverse range of conditions encountered in vivo, such as oxygen tension, environmental pH and nutrient availability. Although nitrogen is an essential nutrient for every organism, little is known about the genes and pathways responsible for nitrogen assimilation in M . tuberculosis. In this study we have used transcriptomics and chromatin immunoprecipitation and high‐throughput sequencing to address this. In response to nitrogen starvation, a total of 185 genes were significantly differentially expressed (96 up‐regulated and 89 down regulated; 5% genome) highlighting several significant areas of metabolic change during nitrogen limitation such as nitrate/nitrite metabolism, aspartate metabolism and changes in cell wall biosynthesis. We identify GlnR as a regulator involved in the nitrogen response, controlling the expression of at least 33 genes in response to nitrogen limitation. We identify a consensus GlnR binding site and relate its location to known transcriptional start sites. We also show that the GlnR response regulator plays a very different role in M . tuberculosis to that in non‐pathogenic mycobacteria, controlling genes involved in nitric oxide detoxification and intracellular survival instead of genes involved in nitrogen scavenging. PMID:26077160

Flexible Microsensor Array for the Root Zone Monitoring of Porous Tube Plant Growth System

NASA Technical Reports Server (NTRS)

Sathyan, Sandeep; Kim, Chang-Soo; Porterfield, D. Marshall; Nagle, H. Troy; Brown, Christopher S.

2004-01-01

Control of oxygen and water in the root zone is vital to support plant growth in the microgravity environment. The ability to control these sometimes opposing parameters in the root zone is dependent upon the availability of sensors to detect these elements and provide feedback for control systems. In the present study we demonstrate the feasibility of using microsensor arrays on a flexible substrate for dissolved oxygen detection, and a 4-point impedance microprobe for surface wetness detection on the surface of a porous tube (PT) nutrient delivery system. The oxygen microsensor reported surface oxygen concentrations that correlated with the oxygen concentrations of the solution inside the PT when operated at positive pressures. At negative pressures the microsensor shows convergence to zero saturation (2.2 micro mol/L) values due to inadequate water film formation on porous tube surface. The 4-point microprobe is useful as a wetness detector as it provides a clear differentiation between dry and wet surfaces. The unique features of the dissolved oxygen microsensor array and 4-point microprobe include small and simple design, flexibility and multipoint sensing. The demonstrated technology is anticipated to provide low cost, and highly reliable sensor feedback monitoring plant growth nutrient delivery system in both terrestrial and microgravity environments.

Crosstalk between gastrointestinal neurons and the brain in the control of food intake.

PubMed

Mithieux, Gilles

2014-10-01

Recent data have emphasized that the gastrointestinal nervous system is preponderant in the sensing of nutrients and hormones and its translation in terms of control of food intake by the central nervous system. More specifically, the gastrointestinal neural system participates in the control of hunger via the sensing of at least two major macronutrients, e.g. glucose and protein, which may control hunger sensations from the portal vein. Protein are first sensed by mu-opioid receptors present in the portal vein walls to induce intestinal gluconeogenesis-via a reflex arc and next portal glucose sensing. The gastrointestinal nervous system may also account for the rapid benefits of gastric bypass surgeries on energy homeostasis (hunger and body weight) and glucose homeostasis (insulin sensitivity). This knowledge provides novel mechanisms of control of body weight, which might be useful to envision future approaches of prevention or treatment of obesity. Copyright © 2014 Elsevier Ltd. All rights reserved.

Hypothalamic glucose-sensing: role of Glia-to-neuron signaling.

PubMed

Tonon, M C; Lanfray, D; Castel, H; Vaudry, H; Morin, F

2013-12-01

The hypothalamus senses hormones and nutrients in order to regulate energy balance. In particular, detection of hypothalamic glucose levels has been shown to regulate both feeding behavior and peripheral glucose homeostasis, and impairment of this regulatory system is believed to be involved in the development of obesity and diabetes. Several data clearly demonstrate that glial cells are key elements in the perception of glucose, constituting with neurons a "glucose-sensing unit". Characterization of this interplay between glia and neurons represents an exciting challenge, and will undoubtedly contribute to identify new candidates for therapeutic intervention. The purpose of this review is to summarize the current data that stress the importance of glia in central glucose-sensing. The nature of the glia-to-neuron signaling is discussed, with a special focus on the endozepine ODN, a potent anorexigenic peptide that is highly expressed in hypothalamic glia. © Georg Thieme Verlag KG Stuttgart · New York.

Sequential nutrient uptake as a potential mechanism for phytoplankton to maintain high primary productivity and balanced nutrient stoichiometry

NASA Astrophysics Data System (ADS)

Yin, Kedong; Liu, Hao; Harrison, Paul J.

2017-05-01

We hypothesize that phytoplankton have the sequential nutrient uptake strategy to maintain nutrient stoichiometry and high primary productivity in the water column. According to this hypothesis, phytoplankton take up the most limiting nutrient first until depletion, continue to draw down non-limiting nutrients and then take up the most limiting nutrient rapidly when it is available. These processes would result in the variation of ambient nutrient ratios in the water column around the Redfield ratio. We used high-resolution continuous vertical profiles of nutrients, nutrient ratios and on-board ship incubation experiments to test this hypothesis in the Strait of Georgia. At the surface in summer, ambient NO3- was depleted with excess PO43- and SiO4- remaining, and as a result, both N : P and N : Si ratios were low. The two ratios increased to about 10 : 1 and 0. 45 : 1, respectively, at 20 m. Time series of vertical profiles showed that the leftover PO43- continued to be removed, resulting in additional phosphorus storage by phytoplankton. The N : P ratios at the nutricline in vertical profiles responded differently to mixing events. Field incubation of seawater samples also demonstrated the sequential uptake of NO3- (the most limiting nutrient) and then PO43- and SiO4- (the non-limiting nutrients). This sequential uptake strategy allows phytoplankton to acquire additional cellular phosphorus and silicon when they are available and wait for nitrogen to become available through frequent mixing of NO3- (or pulsed regenerated NH4). Thus, phytoplankton are able to maintain high productivity and balance nutrient stoichiometry by taking advantage of vigorous mixing regimes with the capacity of the stoichiometric plasticity. To our knowledge, this is the first study to show the in situ dynamics of continuous vertical profiles of N : P and N : Si ratios, which can provide insight into the in situ dynamics of nutrient stoichiometry in the water column and the inference of the transient status of phytoplankton nutrient stoichiometry in the coastal ocean.

Improving soil nutrient availability increases carbon rhizodeposition under maize and soybean in Mollisols.

PubMed

Qiao, Yunfa; Miao, Shujie; Han, Xiaozeng; Yue, Shuping; Tang, Caixian

2017-12-15

Rhizodeposited carbon (C) is an important source of soil organic C, and plays an important role in the C cycle in the soil-plant-atmosphere continuum. However, interactive effects of plant species and soil nutrient availability on C rhizodeposition remain unclear. This experiment examined the effect of soil nutrient availability on C rhizodeposition of C4 maize and C3 soybean with contrasting photosynthetic capacity. The soils (Mollisols) were collected from three treatments of no fertilizer (Control), inorganic fertilizer only (NPK), and NPK plus organic manure (NPKM) in a 24-year fertilization field trial. The plants were labelled with 13 C at the vegetative and reproductive stages. The 13 C abundance of shoots, roots and soil were quantified at 0, 7days after 13 C labelling, and at maturity. Increasing soil nutrient availability enhanced the C rhizodeposition due to the greater C fixation in shoots and distribution to roots and soil. The higher amount of averaged below-ground C allocated to soil resulted in greater specific rhizodeposited C from soybean than maize. Additional organic amendment further enhanced them. As a result, higher soil nutrient availability increased total soil organic C under both maize and soybean systems though there was no significant difference between the two crop systems. All these suggested that higher soil nutrient availability favors C rhizodeposition. Mean 80, 260 and 300kgfixedCha -1 were estimated to transfer into soil in the Control, NPK and NPKM treatments, respectively, during one growing season. Copyright © 2017 Elsevier B.V. All rights reserved.

Investigating Stream Metabolism and Nutrient Dynamics in Contrasting Ecosystems: The Role of Hydrologic Compartments

NASA Astrophysics Data System (ADS)

Gonzalez-Pinzon, R.; Riveros-Iregui, D. A.; Covino, T. P.

2015-12-01

The interactions between mobile and less mobile hydrologic compartments affect the quality and quantity of water in streams and aquifers, and the cycling of dissolved carbon and nutrients. As new laboratory and field techniques become available, new questions and challenges emerge, including: What do we measure, where, and for how long to fully characterize a system? and, What is the ideal cost-maintenance-benefit relationship that we should strive for to maximize knowledge gained in different field settings? We recently performed a series of field experiments to measure aquatic metabolism and nutrient dynamics in two highly contrasting hydrologic systems, i.e., 1) a wetland-stream alpine, tropical system in Colombia (South America) and 2) a dryland river continuum (1st - 5th stream orders) in New Mexico. In this presentation we discuss how multiple lines of evidence can support the analysis of key aquatic processes and how co-interpretation provides a more complete picture of stream complexity. For this analysis, we deployed YSI EXO2 and 6920 sondes, Turner Designs C-sense and C6 sensors, and Onset HOBO water quality data loggers. Parameters measured by these instruments include conductivity, temperature, dissolved oxygen, pH, turbidity, pCO2, chlorophyll-a, phycocyanin, fluorescein, CDOM, brighteners and water depth. We also injected conservative tracers (i.e., NaCl and NaBr) and the bioreactive tracer resazurin in both experimental sites, and NO3 in the dryland river continuum. NO3 was measured in-situ with Satlantic Submersible Ultraviolet Nitrate Analyzers (SUNA) sensors and in the laboratory using Ion Chromatograph techniques using stream grab samples. Our results highlight the role of both residence times and chemical fluxes in regulating the effective processing of carbon and nutrients. Our results also demonstrate that stream stimuli from controlled experiments are ideal for maximizing the information content derived from short (hours to days) and mid-term (weeks) sensor deployment campaigns.

Interactive Effects of Nutrient and Mechanical Stresses on Plant Morphology

PubMed Central

Puijalon, Sara; Lena, Jean-Paul; Bornette, Gudrun

2007-01-01

Background and Aims Plant species frequently encounter multiple stresses under natural conditions, and the way they cope with these stresses is a major determinant of their ecological breadth. The way mechanical (e.g. wind, current) and resource stresses act simultaneously on plant morphological traits has been poorly addressed, even if both stresses often interact. This paper aims to assess whether hydraulic stress affects plant morphology in the same way at different nutrient levels. Methods An examination was made of morphological variations of an aquatic plant species growing under four hydraulic stress (flow velocity) gradients located in four habitats distributed along a nutrient gradient. Morphological traits covering plant size, dry mass allocation, organ water content and foliage architecture were measured. Key Results Significant interactive effects of flow velocity and nutrient level were observed for all morphological traits. In particular, increased flow velocity resulted in size reductions under low nutrient conditions, suggesting an adaptive response to flow stress (escape strategy). On the other hand, moderate increases in flow velocity resulted in increased size under high nutrient conditions, possibly related to an inevitable growth response to a higher nutrient supply induced by water renewal at the plant surface. For some traits (e.g. dry mass allocation), a consistent sense of variation as a result of increasing flow velocity was observed, but the amount of variation was either reduced or amplified under nutrient-rich compared with nutrient-poor conditions, depending on the traits considered. Conclusions These results suggest that, for a given species, a stress factor may result, in contrasting patterns and hence strategies, depending on a second stress factor. Such results emphasize the relevance of studies on plant responses to multiple stresses for understanding the actual ecological breadth of species. PMID:17913725

When microbes and consumers determine the limiting nutrient of autotrophs: a theoretical analysis

PubMed Central

Cherif, Mehdi; Loreau, Michel

2008-01-01

Ecological stoichiometry postulates that differential nutrient recycling of elements such as nitrogen and phosphorus by consumers can shift the element that limits plant growth. However, this hypothesis has so far considered the effect of consumers, mostly herbivores, out of their food-web context. Microbial decomposers are important components of food webs, and might prove as important as consumers in changing the availability of elements for plants. In this theoretical study, we investigate how decomposers determine the nutrient that limits plants, both by feeding on nutrients and organic carbon released by plants and consumers, and by being fed upon by omnivorous consumers. We show that decomposers can greatly alter the relative availability of nutrients for plants. The type of limiting nutrient promoted by decomposers depends on their own elemental composition and, when applicable, on their ingestion by consumers. Our results highlight the limitations of previous stoichiometric theories of plant nutrient limitation control, which often ignored trophic levels other than plants and herbivores. They also suggest that detrital chains play an important role in determining plant nutrient limitation in many ecosystems. PMID:18854301

Nitrogenase and Alkaline Phosphatase Activity in Wetland Metaphyton: Implications for Primary Production and CNP Composition

NASA Astrophysics Data System (ADS)

Scott, T.; Doyle, R.

2005-05-01

Longitudinal gradients of nutrient availability often occur along the flow path of water in freshwater wetlands. Differential removal efficiencies of water column nitrogen (N) and phosphorus (P) may increase the severity of nutrient deficiency and possibly change the nutrient that limits primary production. A previous study demonstrated that periphyton in the Lake Waco Wetlands (LWW), near Waco, Texas, USA, are generally more P limited near the inflow and become increasingly N limited as distance from the inflow increases. Therefore, spatial heterogeneity in nutrient availability likely influences both the structure and function of periphyton assemblages within this system. In this ongoing study, we are evaluating the relationships between metaphyton primary production, nitrogenase activity, alkaline phosphatase activity, and CNP stoichiometry in areas of differing nutrient limitation within the LWW. As expected, primary production is generally greatest in areas where nitrogenase and alkaline phosphatase activities are minimal. However, expected increases in C:N ratios in areas of greatest nutrient deficiency have not been frequently observed. Decreased primary production and increased enzyme mediated nutrient uptake appear to balance metaphyton nutrient content in these areas.

Mitochondria-associated endoplasmic reticulum membranes allow adaptation of mitochondrial metabolism to glucose availability in the liver.

PubMed

Theurey, Pierre; Tubbs, Emily; Vial, Guillaume; Jacquemetton, Julien; Bendridi, Nadia; Chauvin, Marie-Agnès; Alam, Muhammad Rizwan; Le Romancer, Muriel; Vidal, Hubert; Rieusset, Jennifer

2016-04-01

Mitochondria-associated endoplasmic reticulum membranes (MAM) play a key role in mitochondrial dynamics and function and in hepatic insulin action. Whereas mitochondria are important regulators of energy metabolism, the nutritional regulation of MAM in the liver and its role in the adaptation of mitochondria physiology to nutrient availability are unknown. In this study, we found that the fasted to postprandial transition reduced the number of endoplasmic reticulum-mitochondria contact points in mouse liver. Screening of potential hormonal/metabolic signals revealed glucose as the main nutritional regulator of hepatic MAM integrity both in vitro and in vivo Glucose reduced organelle interactions through the pentose phosphate-protein phosphatase 2A (PP-PP2A) pathway, induced mitochondria fission, and impaired respiration. Blocking MAM reduction counteracted glucose-induced mitochondrial alterations. Furthermore, disruption of MAM integrity mimicked effects of glucose on mitochondria dynamics and function. This glucose-sensing system is deficient in the liver of insulin-resistant ob/ob and cyclophilin D-KO mice, both characterized by chronic disruption of MAM integrity, mitochondrial fission, and altered mitochondrial respiration. These data indicate that MAM contribute to the hepatic glucose-sensing system, allowing regulation of mitochondria dynamics and function during nutritional transition. Chronic disruption of MAM may participate in hepatic mitochondrial dysfunction associated with insulin resistance. © The Author (2016). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. All rights reserved.

Glucose and hypothalamic astrocytes: More than a fueling role?

PubMed

Leloup, C; Allard, C; Carneiro, L; Fioramonti, X; Collins, S; Pénicaud, L

2016-05-26

Brain plays a central role in energy homeostasis continuously integrating numerous peripheral signals such as circulating nutrients, and in particular blood glucose level, a variable that must be highly regulated. Then, the brain orchestrates adaptive responses to modulate food intake and peripheral organs activity in order to achieve the fine tuning of glycemia. More than fifty years ago, the presence of glucose-sensitive neurons was discovered in the hypothalamus, but what makes them specific and identifiable still remains disconnected from their electrophysiological signature. On the other hand, astrocytes represent the major class of macroglial cells and are now recognized to support an increasing number of neuronal functions. One of these functions consists in the regulation of energy homeostasis through neuronal fueling and nutrient sensing. Twenty years ago, we discovered that the glucose transporter GLUT2, the canonical "glucosensor" of the pancreatic beta-cell together with the glucokinase, was also present in astrocytes and participated in hypothalamic glucose sensing. Since then, many studies have identified other actors and emphasized the astroglial participation in this mechanism. Growing evidence suggest that astrocytes form a complex network and have to be considered as spatially coordinated and regulated metabolic units. In this review we aim to provide an updated view of the molecular and respective cellular pathways involved in hypothalamic glucose sensing, and their relevance in physiological and pathological states. Copyright © 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

Taste sensing in the colon.

PubMed

Kaji, Izumi; Karaki, Shin-ichiro; Kuwahara, Atsukazu

2014-01-01

The colonic lumen is continually exposed to many compounds, including beneficial and harmful compounds that are produced by colonic microflora. The intestinal epithelia form a barrier between the internal and luminal (external) environments. Chemical receptors that sense the luminal environment are thought to play important roles as sensors and as modulators of epithelial cell functions. The recent molecular identification of various membrane receptor proteins has revealed the sensory role of intestinal epithelial cells. Nutrient sensing by these receptors in the small intestine is implicated in nutrient absorption and metabolism. However, little is known about the physiological roles of chemosensors in the large intestine. Since 1980s, researchers have examined the effects of short-chain fatty acids (SCFA), the primary products of commensal bacteria, on gut motility, secretion, and incretin release, for example. In this decade, the SCFA receptor genes and their expression were identified in the mammalian colon. Furthermore, many other chemical receptors, including taste and olfactory receptors have been found in colonic epithelial cells. These findings indicate that the large intestinal epithelia express chemosensors that detect the luminal contents, particularly bacterial metabolites, and induce the host defense systems and the modulation of systemic metabolism via incretin release. In this review, we describe the local effects of chemical stimuli on the lumen associated with the expression pattern of sensory receptors. We propose that sensory receptors expressed in the colonic mucosa play important roles in luminal chemosensing to maintain homeostasis.

Remote sensing in precision farming: real-time monitoring of water and fertilizer requirements of agricultural crops

NASA Astrophysics Data System (ADS)

Zilberman, Arkadi; Ben Asher, Jiftah; Kopeika, Norman S.

2016-10-01

The advancements in remote sensing in combination with sensor technology (both passive and active) enable growers to analyze an entire crop field as well as its local features. In particular, changes of actual evapo-transpiration (ET) as a function of water availability can be measured remotely with infrared radiometers. Detection of crop water stress and ET and combining it with the soil water flow model enable rational irrigation timing and application amounts. Nutrient deficiency, and in particular nitrogen deficiency, causes substantial crop losses. This deficiency needs to be identified immediately. A faster the detection and correction, a lesser the damage to the crop yield. In the present work, to retrieve ET a novel deterministic approach was used which is based on the remote sensing data. The algorithm can automatically provide timely valuable information on plant and soil water status, which can improve the management of irrigated crops. The solution is capable of bridging between Penman-Monteith ET model and Richards soil water flow model. This bridging can serve as a preliminary tool for expert irrigation system. To support decisions regarding fertilizers the greenness of plant canopies is assessed and quantified by using the spectral reflectance sensors and digital color imaging. Fertilization management can be provided on the basis of sampling and monitoring of crop nitrogen conditions using RS technique and translating measured N concentration in crop to kg/ha N application in the field.

Nutrient availability and nutrient use efficiency in plants growing in the transition zone between land and water.

PubMed

Cavalli, G; Baattrup-Pedersen, A; Riis, T

2016-03-01

The transition zone between terrestrial and freshwater habitats is highly dynamic, with large variability in environmental characteristics. Here, we investigate how these characteristics influence the nutritional status and performance of plant life forms inhabiting this zone. Specifically, we hypothesised that: (i) tissue nutrient content differs among submerged, amphibious and terrestrial species, with higher content in submerged species; and (ii) PNUE gradually increases from submerged over amphibious to terrestrial species, reflecting differences in the availability of N and P relative to inorganic C across the land-water ecotone. We found that tissue nutrient content was generally higher in submerged species and C:N and C:P ratios indicated that content was limiting for growth for ca. 20% of plant individuals, particularly those belonging to amphibious and terrestrial species groups. As predicted, the PNUE increased from submerged over amphibious to terrestrial species. We suggest that this pattern reflects that amphibious and terrestrial species allocate proportionally more nutrients into processes of importance for photosynthesis at saturating CO2 availability, i.e. enzymes involved in substrate regeneration, compared to submerged species that are acclimated to lower availability of CO2 in the aquatic environment. Our results indicate that enhanced nutrient loading may affect relative abundance of the three species groups in the land-water ecotone of stream ecosystems. Thus, species of amphibious and terrestrial species groups are likely to benefit more from enhanced nutrient availability in terms of faster growth compared to aquatic species, and that this can be detrimental to aquatic species growing in the land-water ecotone, e.g. Ranunculus and Callitriche. © 2015 German Botanical Society and The Royal Botanical Society of the Netherlands.

Relationship between soil nutrients and mycorrhizal associations of two Bipinnula species (Orchidaceae) from central Chile.

PubMed

Mujica, María Isabel; Saez, Nicolás; Cisternas, Mauricio; Manzano, Marlene; Armesto, Juan J; Pérez, Fernanda

2016-07-01

Mycorrhizal associations are influenced by abiotic and biotic factors, including climate, soil conditions and the identity of host plants. However, the effect of environmental conditions on orchid mycorrhizal associations remains poorly understood. The present study examined how differences in soil nutrient availability are related to the diversity and composition of mycorrhizal fungi associated with two terrestrial orchid species from central Chile. For 12 populations of Bipinnula fimbriata and B. plumosa, OTU (operational taxonomic unit) richness, phylogenetic diversity and community composition of mycorrhizal fungi in root samples were estimated using internal transcribed spacer (ITS) sequences. Then, these mycorrhizal diversity variables were related to soil nutrients and host species using generalized linear models and non-metric multidimensional scaling. Variation in OTU composition of mycorrhizal fungi among sites was explained mainly by orchid host species. Fungi in Tulasnellaceae and Ceratobasidiaceae were isolated from both orchid species, but the former were more frequent in B. fimbriata and the latter in B. plumosa. Soil nutrients and orchid host species had significant effects on OTU richness and phylogenetic diversity. Mycorrhizal diversity decreased in habitats with higher N in both species and increased with P availability only in B. fimbriata The results suggest that soil nutrient availability modulates orchid mycorrhizal associations and provide support for the hypothesis that specialization is favoured by higher soil nutrient availability. Inter-specific differences in mycorrhizal composition can arise due to a geographical pattern of distribution of orchid mycorrhizal fungi, host preferences for fungal partners or differential performance of mycorrhizal fungi under different nutrient availabilities. Further experiments are needed to evaluate these hypotheses. © The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Relationship between soil nutrients and mycorrhizal associations of two Bipinnula species (Orchidaceae) from central Chile

PubMed Central

Mujica, María Isabel; Saez, Nicolás; Cisternas, Mauricio; Manzano, Marlene; Armesto, Juan J.; Pérez, Fernanda

2016-01-01

Background and Aims Mycorrhizal associations are influenced by abiotic and biotic factors, including climate, soil conditions and the identity of host plants. However, the effect of environmental conditions on orchid mycorrhizal associations remains poorly understood. The present study examined how differences in soil nutrient availability are related to the diversity and composition of mycorrhizal fungi associated with two terrestrial orchid species from central Chile. Methods For 12 populations of Bipinnula fimbriata and B. plumosa, OTU (operational taxonomic unit) richness, phylogenetic diversity and community composition of mycorrhizal fungi in root samples were estimated using internal transcribed spacer (ITS) sequences. Then, these mycorrhizal diversity variables were related to soil nutrients and host species using generalized linear models and non-metric multidimensional scaling. Key Results Variation in OTU composition of mycorrhizal fungi among sites was explained mainly by orchid host species. Fungi in Tulasnellaceae and Ceratobasidiaceae were isolated from both orchid species, but the former were more frequent in B. fimbriata and the latter in B. plumosa. Soil nutrients and orchid host species had significant effects on OTU richness and phylogenetic diversity. Mycorrhizal diversity decreased in habitats with higher N in both species and increased with P availability only in B. fimbriata. Conclusions The results suggest that soil nutrient availability modulates orchid mycorrhizal associations and provide support for the hypothesis that specialization is favoured by higher soil nutrient availability. Inter-specific differences in mycorrhizal composition can arise due to a geographical pattern of distribution of orchid mycorrhizal fungi, host preferences for fungal partners or differential performance of mycorrhizal fungi under different nutrient availabilities. Further experiments are needed to evaluate these hypotheses. PMID:27311572

Design and implementation of sensor systems for control of a closed-loop life support system

NASA Technical Reports Server (NTRS)

Alnwick, Leslie; Clark, Amy; Debs, Patricia; Franczek, Chris; Good, Tom; Rodrigues, Pedro

1989-01-01

The sensing and controlling needs for a Closed-Loop Life Support System (CLLSS) were investigated. The sensing needs were identified in five particular areas and the requirements were defined for workable sensors. The specific areas of interest were atmosphere and temperature, nutrient delivery, plant health, plant propagation and support, and solids processing. The investigation of atmosphere and temperature control focused on the temperature distribution within the growth chamber as well as the possibility for sensing other parameters such as gas concentration, pressure, and humidity. The sensing needs were studied for monitoring the solution level in a porous membrane material along with the requirements for measuring the mass flow rate in the delivery system. The causes and symptoms of plant disease were examined and the various techniques for sensing these health indicators were explored. The study of sensing needs for plant propagation and support focused on monitoring seed viability and measuring seed moisture content as well as defining the requirements for drying and storing the seeds. The areas of harvesting, food processing, and resource recycling, were covered with a main focus on the sensing possibilities for regulating the recycling process.

Comparative Sensor Fusion between Hyperspectral and Multispectral Remote Sensing Data for Monitoring Microcystin Distribution in Lake Erie

EPA Science Inventory

Urban growth and agricultural production have caused an influx of nutrients into Lake Erie, leading to eutrophic zones. These conditions result in the formation of algal blooms, some of which are toxic due to the presence of Microcystis (a cyanobacteria), which produces the hepat...

Satellite Remote Sensing of Chlorophyll a in Support of Nutrient Management in the Neuse and Tar-Pamlico River Estuaries

EPA Science Inventory

The North Carolina Environmental Management Commission (EMC) has adopted as a water quality standard that chlorophyll a concentration should not exceed 40 ug/L in sounds, estuaries and other slow-moving waters. Exceedances require regulators to develop a Total Maximum Daily Limit...

Satellite remote sensing of chlorophyll a in support of nutrient management in the Neuse and Tar-Pamlico River (North Carolina) estuaries

EPA Science Inventory

The North Carolina Environmental Management Commission (EMC) has adopted as a water quality standard that chlorophyll a concentration should not exceed 40 ug/L in sounds, estuaries and other slow-moving waters. Exceedances require regulators to develop a Total Maximum Daily Limit...

Fly-let biology and the high protein/low carb diet.

PubMed

Rulifson, Eric

2008-04-01

In Drosophila, a simple network of nutrient-sensing neuroendocrine cells, analogs of pancreatic islet alpha and beta cells, regulates carbohydrate metabolism. Work presented in this issue of Cell Metabolism (Buch et al., 2008) shows that signals from these cells control expression of a glycogen-specific glucosidase in response to dietary protein and carbohydrate.

Nutrient sensing pathways as therapeutic targets for healthy ageing.

PubMed

Aiello, Anna; Accardi, Giulia; Candore, Giuseppina; Gambino, Caterina Maria; Mirisola, Mario; Taormina, Giusi; Virruso, Claudia; Caruso, Calogero

2017-04-01

In the present paper, the authors have discussed anti-aging strategies which aim to slow the aging process and to delay the onset of age-related diseases, focusing on nutrient sensing pathways (NSPs) as therapeutic targets. Indeed, several studies have already demonstrated that both in animal models and humans, dietary interventions might have a positive impact on the aging process through the modulation of these pathways. Areas covered: Achieving healthy aging is the main challenge of the twenty-first century because lifespan is increasing, but not in tandem with good health. The authors have illustrated different approaches that can act on NSPs, modulating the rate of the aging process. Expert opinion: Humanity's lasting dream is to reverse or, at least, postpone aging. In recent years, increasing attention has been devoted to anti-aging therapies. The subject is very popular among the general public, whose imagination runs wild with all the possible tools to delay aging and to gain immortality. Some approaches discussed in the present review should be able to substantially slow down the aging process, extending our productive, youthful lives, without frailty.

Incorporating a Constrained Optimization Algorithm into Remote- Sensing/Precision Agriculture Methodology

NASA Astrophysics Data System (ADS)

Morgenthaler, George; Khatib, Nader; Kim, Byoungsoo

with information to improve their crop's vigor has been a major topic of interest. With world population growing exponentially, arable land being consumed by urbanization, and an unfavorable farm economy, the efficiency of farming must increase to meet future food requirements and to make farming a sustainable occupation for the farmer. "Precision Agriculture" refers to a farming methodology that applies nutrients and moisture only where and when they are needed in the field. The goal is to increase farm revenue by increasing crop yield and decreasing applications of costly chemical and water treatments. In addition, this methodology will decrease the environmental costs of farming, i.e., reduce air, soil, and water pollution. Sensing/Precision Agriculture has not grown as rapidly as early advocates envisioned. Technology for a successful Remote Sensing/Precision Agriculture system is now available. Commercial satellite systems can image (multi-spectral) the Earth with a resolution of approximately 2.5 m. Variable precision dispensing systems using GPS are available and affordable. Crop models that predict yield as a function of soil, chemical, and irrigation parameter levels have been formulated. Personal computers and internet access are in place in most farm homes and can provide a mechanism to periodically disseminate, e.g. bi-weekly, advice on what quantities of water and chemicals are needed in individual regions of the field. What is missing is a model that fuses the disparate sources of information on the current states of the crop and soil, and the remaining resource levels available with the decisions farmers are required to make. This must be a product that is easy for the farmer to understand and to implement. A "Constrained Optimization Feed-back Control Model" to fill this void will be presented. The objective function of the model will be used to maximize the farmer's profit by increasing yields while decreasing environmental costs and decreasing application of costly treatments. This model will incorporate information from remote sensing, in-situ weather sources, soil measurements, crop models, and tacit farmer knowledge of the relative productivity of the selected control regions of the farm to provide incremental advice throughout the growing season on water and chemical treatments. Genetic and meta-heuristic algorithms will be used to solve the constrained optimization problem that possesses complex constraints and a non-linear objective function. *

Soil Nutrient Assessment for Urban Ecosystems in Hubei, China

PubMed Central

Li, Zhi-guo; Zhang, Guo-shi; Liu, Yi; Wan, Kai-yuan; Zhang, Run-hua; Chen, Fang

2013-01-01

Recent urban landscape vegetation surveys conducted in many cities in China identified numerous plant nutrient deficiencies, especially in newly developed cities. Soil nutrients and soil nutrient management in the cities of Hubei province have not received adequate attention to date. The aims of this study were to characterize the available nutrients of urban soils from nine cities in Hubei province, China, and to assess how soil nutrient status is related to land use type and topography. Soil nutrients were measured in 405 sites from 1,215 soil samples collected from four land use types (park, institutional [including government building grounds, municipal party grounds, university grounds, and garden city institutes], residential, and roadside verges) and three topographies (mountainous [142–425 m a.s.l], hilly [66–112 m a.s.l], and plain [26–30 m a.s.l]). Chemical analyses showed that urban soils in Hubei had high pH and lower soil organic matter, available nitrogen (N), available phosphorus (P), and available boron (B) concentrations than natural soils. Nutrient concentrations were significantly different among land use types, with the roadside and residential areas having greater concentrations of calcium (Ca), sulfur (S), copper (Cu), manganese (Mn), and zinc (Zn) that were not deficient against the recommended ranges. Topographic comparisons showed statistically significant effects for 8 of the 11 chemical variables (p < 0.05). Concentrations of N, Ca, Mg, S, Cu, and Mn in plain cities were greater than those in mountainous cities and show a negative correlation with city elevation. These results provide data on urban soils characteristics in land use types and topography, and deliver significant information for city planners and policy makers. PMID:24086647

Soil nutrient assessment for urban ecosystems in Hubei, China.

PubMed

Li, Zhi-Guo; Zhang, Guo-Shi; Liu, Yi; Wan, Kai-Yuan; Zhang, Run-Hua; Chen, Fang

2013-01-01

Recent urban landscape vegetation surveys conducted in many cities in China identified numerous plant nutrient deficiencies, especially in newly developed cities. Soil nutrients and soil nutrient management in the cities of Hubei province have not received adequate attention to date. The aims of this study were to characterize the available nutrients of urban soils from nine cities in Hubei province, China, and to assess how soil nutrient status is related to land use type and topography. Soil nutrients were measured in 405 sites from 1,215 soil samples collected from four land use types (park, institutional [including government building grounds, municipal party grounds, university grounds, and garden city institutes], residential, and roadside verges) and three topographies (mountainous [142-425 m a.s.l], hilly [66-112 m a.s.l], and plain [26-30 m a.s.l]). Chemical analyses showed that urban soils in Hubei had high pH and lower soil organic matter, available nitrogen (N), available phosphorus (P), and available boron (B) concentrations than natural soils. Nutrient concentrations were significantly different among land use types, with the roadside and residential areas having greater concentrations of calcium (Ca), sulfur (S), copper (Cu), manganese (Mn), and zinc (Zn) that were not deficient against the recommended ranges. Topographic comparisons showed statistically significant effects for 8 of the 11 chemical variables (p < 0.05). Concentrations of N, Ca, Mg, S, Cu, and Mn in plain cities were greater than those in mountainous cities and show a negative correlation with city elevation. These results provide data on urban soils characteristics in land use types and topography, and deliver significant information for city planners and policy makers.

The influence of soil properties and nutrients on conifer forest growth in Sweden, and the first steps in developing a nutrient availability metric

NASA Astrophysics Data System (ADS)

Van Sundert, Kevin; Horemans, Joanna A.; Stendahl, Johan; Vicca, Sara

2018-06-01

The availability of nutrients is one of the factors that regulate terrestrial carbon cycling and modify ecosystem responses to environmental changes. Nonetheless, nutrient availability is often overlooked in climate-carbon cycle studies because it depends on the interplay of various soil factors that would ideally be comprised into metrics applicable at large spatial scales. Such metrics do not currently exist. Here, we use a Swedish forest inventory database that contains soil data and tree growth data for > 2500 forests across Sweden to (i) test which combination of soil factors best explains variation in tree growth, (ii) evaluate an existing metric of constraints on nutrient availability, and (iii) adjust this metric for boreal forest data. With (iii), we thus aimed to provide an adjustable nutrient metric, applicable for Sweden and with potential for elaboration to other regions. While taking into account confounding factors such as climate, N deposition, and soil oxygen availability, our analyses revealed that the soil organic carbon concentration (SOC) and the ratio of soil carbon to nitrogen (C : N) were the most important factors explaining variation in normalized (climate-independent) productivity (mean annual volume increment - m3 ha-1 yr-1) across Sweden. Normalized forest productivity was significantly negatively related to the soil C : N ratio (R2 = 0.02-0.13), while SOC exhibited an empirical optimum (R2 = 0.05-0.15). For the metric, we started from a (yet unvalidated) metric for constraints on nutrient availability that was previously developed by the International Institute for Applied Systems Analysis (IIASA - Laxenburg, Austria) for evaluating potential productivity of arable land. This IIASA metric requires information on soil properties that are indicative of nutrient availability (SOC, soil texture, total exchangeable bases - TEB, and pH) and is based on theoretical considerations that are also generally valid for nonagricultural ecosystems. However, the IIASA metric was unrelated to normalized forest productivity across Sweden (R2 = 0.00-0.01) because the soil factors under consideration were not optimally implemented according to the Swedish data, and because the soil C : N ratio was not included. Using two methods (each one based on a different way of normalizing productivity for climate), we adjusted this metric by incorporating soil C : N and modifying the relationship between SOC and nutrient availability in view of the observed relationships across our database. In contrast to the IIASA metric, the adjusted metrics explained some variation in normalized productivity in the database (R2 = 0.03-0.21; depending on the applied method). A test for five manually selected local fertility gradients in our database revealed a significant and stronger relationship between the adjusted metrics and productivity for each of the gradients (R2 = 0.09-0.38). This study thus shows for the first time how nutrient availability metrics can be evaluated and adjusted for a particular ecosystem type, using a large-scale database.

Systemic Nutrient and Stress Signaling via Myokines and Myometabolites.

PubMed

Rai, Mamta; Demontis, Fabio

2016-01-01

Homeostatic systems mount adaptive responses to meet the energy demands of the cell and to compensate for dysfunction in cellular compartments. Such surveillance systems are also active at the organismal level: Nutrient and stress sensing in one tissue can lead to changes in other tissues. Here, we review the emerging understanding of the role of skeletal muscle in regulating physiological homeostasis and disease progression in other tissues. Muscle-specific genetic interventions can induce systemic effects indirectly, via changes in the mass and metabolic demand of muscle, and directly, via the release of muscle-derived cytokines (myokines) and metabolites (myometabolites) in response to nutrients and stress. In turn, myokines and myometabolites signal to various target tissues in an autocrine, paracrine, and endocrine manner, thereby determining organismal resilience to aging, disease, and environmental challenges. We propose that tailoring muscle systemic signaling by modulating myokine and myometabolite levels may combat many degenerative diseases and delay aging.

Scaling of physical constraints at the root-soil interface to macroscopic patterns of nutrient retention in ecosystems.

PubMed

Gerber, Stefan; Brookshire, E N Jack

2014-03-01

Nutrient limitation in terrestrial ecosystems is often accompanied with maintaining a nearly closed vegetation-soil nutrient cycle. The ability to retain nutrients in an ecosystem requires the capacity of the plant-soil system to draw down nutrient levels in soils effectually such that export concentrations in soil solutions remain low. Here we address the physical constraints of plant nutrient uptake that may be limited by the diffusive movement of nutrients in soils, by the uptake at the root/mycorrhizal surface, and from interactions with soil water flow. We derive an analytical framework of soil nutrient transport and uptake and predict levels of plant available nutrient concentration and residence time. Our results, which we evaluate for nitrogen, show that the physical environment permits plants to lower soil solute concentration substantially. Our analysis confirms that plant uptake capacities in soils are considerable, such that water movement in soils is generally too small to significantly erode dissolved plant-available nitrogen. Inorganic nitrogen concentrations in headwater streams are congruent with the prediction of our theoretical framework. Our framework offers a physical-based parameterization of nutrient uptake in ecosystem models and has the potential to serve as an important tool toward scaling biogeochemical cycles from individual roots to landscapes.

The stoichiometry of nutrient release by terrestrial herbivores and its ecosystem consequences

NASA Astrophysics Data System (ADS)

Sitters, Judith; Bakker, Elisabeth S.; Veldhuis, Michiel P.; Veen, G. F.; Olde Venterink, Harry; Vanni, Michael J.

2017-04-01

It is widely recognized that the release of nutrients by herbivores via their waste products strongly impacts nutrient availability for autotrophs. The ratios of nitrogen (N) and phosphorus (P) recycled through herbivore release (i.e., waste N:P) are mainly determined by the stoichiometric composition of the herbivore’s food (food N:P) and its body nutrient content (body N:P). Waste N:P can in turn impact autotroph nutrient limitation and productivity. Herbivore-driven nutrient recycling based on stoichiometric principles is dominated by theoretical and experimental research in freshwater systems, in particular interactions between algae and invertebrate herbivores. In terrestrial ecosystems, the impact of herbivores on nutrient cycling and availability is often limited to studying carbon (C ):N and C:P ratios, while the role of terrestrial herbivores in mediating N:P ratios is also likely to influence herbivore-driven nutrient recycling. In this review, we use rules and predictions on the stoichiometry of nutrient release originating from algal-based aquatic systems to identify the factors that determine the stoichiometry of nutrient release by herbivores. We then explore how these rules can be used to understand the stoichiometry of nutrient release by terrestrial herbivores, ranging from invertebrates to mammals, and its impact on plant nutrient limitation and productivity. Future studies should focus on measuring both N and P when investigating herbivore-driven nutrient recycling in terrestrial ecosystems, while also taking the form of waste product (urine or feces) and other pathways by which herbivores change nutrients into account, to be able to quantify the impact of waste stoichiometry on plant communities.

Geographic variation in the relationships of temperature, salinity or sigma sub t versus plant nutrient concentrations in the world ocean. [silicic acid, nitrate, and phosphate concentration

NASA Technical Reports Server (NTRS)

Kamykowski, D.; Zentara, S. J.

1985-01-01

A NODC data set representing all regions of the world ocean was analyzed for temperature and sigma-t relationships with nitrate, phosphate or silicic acid. Six cubic regressions were for each ten degree square of latitude and longitude containing adequate data. World maps display the locations that allow the prediction of plant nutrient concentrations from temperature or sigma-t. Geographic coverage improves along the sequence: nitrate, phosphate, and silicic acid and is better for sigma-t than for temperature. Contour maps of the approximate temperature of sigma-t at which these nitrients are no longer measurable in a parcel of water are generated, based on a percentile analysis of the temperature or sigma-t at which less than a selected amount of plant nutrient occurs. Results are stored on magnetic tape in tabular form. The global potential to predict plant nutrient concentrations from remotely sensed temperature of sigma-t and to emphasize the latitudinally and longitudinally changing phytoplankton growth environment in present and past oceans is demonstrated.

Detection of Anomalies in Citrus Leaves Using Laser-Induced Breakdown Spectroscopy (LIBS).

PubMed

Sankaran, Sindhuja; Ehsani, Reza; Morgan, Kelly T

2015-08-01

Nutrient assessment and management are important to maintain productivity in citrus orchards. In this study, laser-induced breakdown spectroscopy (LIBS) was applied for rapid and real-time detection of citrus anomalies. Laser-induced breakdown spectroscopy spectra were collected from citrus leaves with anomalies such as diseases (Huanglongbing, citrus canker) and nutrient deficiencies (iron, manganese, magnesium, zinc), and compared with those of healthy leaves. Baseline correction, wavelet multivariate denoising, and normalization techniques were applied to the LIBS spectra before analysis. After spectral pre-processing, features were extracted using principal component analysis and classified using two models, quadratic discriminant analysis and support vector machine (SVM). The SVM resulted in a high average classification accuracy of 97.5%, with high average canker classification accuracy (96.5%). LIBS peak analysis indicated that high intensities at 229.7, 247.9, 280.3, 393.5, 397.0, and 769.8 nm were observed of 11 peaks found in all the samples. Future studies using controlled experiments with variable nutrient applications are required for quantification of foliar nutrients by using LIBS-based sensing.

[Nutrient content and adequacy of roughage for horse nutrition: an investigation under Swiss conditions].

PubMed

Nater, S; Wanner, M; Wichert, B

2007-03-01

For horses no special tables related to nutrients for Swiss roughage exist. For this reason samples of hay, straw, silage/haylage and green forage were taken from 46 horse keeping farms in 22 cantons. The samples were judged by sense and following the nutrient--and macromineral--content as well as the content of fructans were analysed. Regarding its quality no sample was totally inadequate for horses. The mean contents of crude protein in Swiss hay for horses were clearly lower than in hay for ruminants and in German hay for horses. The mineral contents (calcium, magnesium, phosphorus) showed a wide range. On average they were also lower than the values provided in tables for ruminants. Except for one sample the silages/haylages showed a dry matter content of more than 40 %. No nutrient tables for silage or haylage, which are such high in dry matter contents, were found in the literature. The contents offructans in silage/haylage and green forage also showed a wide range with a maximum of 94 g/kg DM fructan.

Cell-specific CO2 fixation rates of two distinct groups of plastidic protists in the Atlantic Ocean remain unchanged after nutrient addition.

PubMed

Grob, Carolina; Jardillier, Ludwig; Hartmann, Manuela; Ostrowski, Martin; Zubkov, Mikhail V; Scanlan, David J

2015-04-01

To assess the role of open-ocean ecosystems in global CO2 fixation, we investigated how picophytoplankton, which dominate primary production, responded to episodic increases in nutrient availability. Previous experiments have shown nitrogen alone, or in combination with phosphorus or iron, to be the proximate limiting nutrient(s) for total phytoplankton grown over several days. Much less is known about how nutrient upshift affects picophytoplankton CO2 fixation over the duration of the light period. To address this issue, we performed a series of small volume (8-60 ml) - short term (10-11 h) nutrient addition experiments in different regions of the Atlantic Ocean using NH4 Cl, FeCl3 , K medium, dust and nutrient-rich water from 300 m depth. We found no significant nutrient stimulation of group-specific CO2 fixation rates of two taxonomically and size-distinct groups of plastidic protists. The above was true regardless of the region sampled or nutrient added, suggesting that this is a generic phenomenon. Our findings show that at least in the short term (i.e. daylight period), nutrient availability does not limit CO2 fixation by the smallest plastidic protists, while their taxonomic composition does not determine their response to nutrient addition. © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.

Can nutrient limitations explain low and declining white spruce growth near the Arctic treeline in the eastern Brooks Range, Alaska?

NASA Astrophysics Data System (ADS)

Ellison, S.; Sullivan, P. F.

2014-12-01

The position of the Arctic treeline is of critical importance for global carbon cycling and surface energy budgets. However, controls on tree growth at treeline remain uncertain. In the Alaskan Brooks Range, 20th century warming has caused varying growth responses among treeline trees, with trees in the west responding positively, while trees in the east have responded negatively. The prevailing explanation of this trend ascribes the negative growth response to warming-induced drought stress in the eastern Brooks Range. However, recent measurements of carbon isotope discrimination in tree rings, xylem sap flow and needle gas exchange suggest that drought stress cannot explain these regional growth declines. Additionally, evidence from the western Brooks Range suggests that nutrient availability, rather than drought stress, may be the proximate control on tree growth. In this study, we investigated the hypothesis that low and declining growth of eastern Brooks Range trees is due to low and declining soil nutrient availability, which may continue to decrease with climate change as soils become drier and microbial activity declines. We compared microclimate, tree performance, and a wide range of proxies for soil nutrient availability in four watersheds along a west-east transect in the Brooks Range during the growing seasons of 2013 and 2014. We hypothesized that soil nutrient availability would track closely with the strong west-east precipitation gradient, with higher rainfall and greater soil nutrient availability in the western Brooks Range. We expected to find that soil water contents in the west are near optimum for nitrogen mineralization, while those in the east are below optimum. Needle nitrogen concentration, net photosynthesis, branch extension growth, and growth in the main stem are expected to decline with the hypothesized decrease in soil nutrient availability. The results of our study will elucidate the current controls on growth of trees near the Arctic treeline, enabling improved predictions of future treeline position and more accurate reconstructions of past climate.

Brain glucose sensing in homeostatic and hedonic regulation.

PubMed

Steinbusch, Laura; Labouèbe, Gwenaël; Thorens, Bernard

2015-09-01

Glucose homeostasis as well as homeostatic and hedonic control of feeding is regulated by hormonal, neuronal, and nutrient-related cues. Glucose, besides its role as a source of metabolic energy, is an important signal controlling hormone secretion and neuronal activity, hence contributing to whole-body metabolic integration in coordination with feeding control. Brain glucose sensing plays a key, but insufficiently explored, role in these metabolic and behavioral controls, which when deregulated may contribute to the development of obesity and diabetes. The recent introduction of innovative transgenic, pharmacogenetic, and optogenetic techniques allows unprecedented analysis of the complexity of central glucose sensing at the molecular, cellular, and neuronal circuit levels, which will lead to a new understanding of the pathogenesis of metabolic diseases. Copyright © 2015 Elsevier Ltd. All rights reserved.

Nutrient limitation and morphological plasticity of the carnivorous pitcher plant Sarracenia purpurea in contrasting wetland environments.

PubMed

Bott, Terry; Meyer, Gretchen A; Young, Erica B

2008-01-01

* Plasticity of leaf nutrient content and morphology, and macronutrient limitation were examined in the northern pitcher plant, Sarracenia purpurea subsp. purpurea, in relation to soil nutrient availability in an open, neutral pH fen and a shady, acidic ombrotrophic bog, over 2 yr following reciprocal transplantation of S. purpurea between the wetlands. * In both wetlands, plants were limited by nitrogen (N) but not phosphorus (P) (N content < 2% DW(-1), N : P < 14) but photosynthetic quantum yields were high (F(V)/F(M) > 0.79). Despite carnivory, leaf N content correlated with dissolved N availability to plant roots (leaf N vs , r(2) = 0.344, P < 0.0001); carnivorous N acquisition did not apparently overcome N limitation. * Following transplantation, N content and leaf morphological traits changed in new leaves to become more similar to plants in the new environment, reflecting wetland nutrient availability. Changes in leaf morphology were faster when plants were transplanted from fen to bog than from bog to fen, possibly reflecting a more stressful environment in the bog. * Morphological plasticity observed in response to changes in nutrient supply to the roots in natural habitats complements previous observations of morphological changes with experimental nutrient addition to pitchers.

Nutrient allocation among plant organs across 13 tree species in three Bornean rain forests with contrasting nutrient availabilities.

PubMed

Aoyagi, Ryota; Kitayama, Kanehiro

2016-07-01

Allocation of nitrogen (N) and phosphorus (P) among plant organs is an important factor regulating growth rate, which is a key ecological process associated with plant life-history strategies. However, few studies have explored how N and P investment in photosynthetic (leaves) and non-photosynthetic (stems and roots) organs changes in relation to depletion of each element. We investigated nutrient concentrations of plant organs in relation to whole-plant nutrient concentration (total nutrient weight per total biomass) as an index of nutrient status of each individual using the saplings of the 13 species in three tropical rain forests with contrasting N and P availabilities (tropical evergreen forests and tropical heath forests). We found a steeper decrease in foliar N concentration than foliar P concentration with decreasing whole-plant nutrient concentration. Moreover, the steeper decrease in foliar N concentration was associated with relatively stable N concentration in stems, and vice versa for P. We suggest that the depletion of N is associated with a rapid dilution of foliar N because the cell walls in non-photosynthetic organs function as an N sink. On the other hand, these species can maintain foliar P concentration by decreasing stem P concentrations despites the depletion of P. Our results emphasize the significance of non-photosynthetic organs as an N sink for understanding the variation of foliar nutrient concentrations for the tree species in the three Bornean rain forests with different N and P availabilities.

Bioavailability of Dissolved Organic Carbon and Nitrogen From Tropical Montane Rainforest Streams Across a Geologic age Gradient

NASA Astrophysics Data System (ADS)

Wiegner, T. N.

2005-05-01

Dissolved organic matter (DOM) is metabolically important in streams. Its bioavailability is influenced by organic matter sources to streams and inorganic nutrient availability. As forest canopies and soils develop over time, organic matter inputs to streams should switch from algal to watershed sources. Across this succession gradient, nutrient limitation should also change. This study examines how chemical composition and bioavailability of DOM from tropical montane rainforest streams on Hawaii change across a geologic age gradient from 4 ky to 150 ky. Dissolved organic C (DOC) and N (DON) concentrations, chemical characteristics, and bioavailability varied with site age. With increasing stream age, DOC and DON concentrations, DOM aromaticity, and the C:N of the stream DOM increased. Changes in stream DOM chemistry and inorganic nutrient availability affected DOM bioavailability. Fifty percent of the DOC from the 4 ky site was bioavailable, where little to none was bioavailable from the older streams. Inorganic nutrient availability did not affect DOC bioavailability. In contrast, DON bioavailability was similar (12%) across sites and was affected by inorganic nutrient availability. This study demonstrates that the chemistry and metabolism of streams draining forests change with ecosystem age and development.

Incorporating a constrained optimization algorithm into remote sensing/precision agriculture methodology

NASA Astrophysics Data System (ADS)

Moreenthaler, George W.; Khatib, Nader; Kim, Byoungsoo

2003-08-01

For two decades now, the use of Remote Sensing/Precision Agriculture to improve farm yields while reducing the use of polluting chemicals and the limited water supply has been a major goal. With world population growing exponentially, arable land being consumed by urbanization, and an unfavorable farm economy, farm efficiency must increase to meet future food requirements and to make farming a sustainable, profitable occupation. "Precision Agriculture" refers to a farming methodology that applies nutrients and moisture only where and when they are needed in the field. The real goal is to increase farm profitability by identifying the additional treatments of chemicals and water that increase revenues more than they increase costs and do no exceed pollution standards (constrained optimization). Even though the economic and environmental benefits appear to be great, Remote Sensing/Precision Agriculture has not grown as rapidly as early advocates envisioned. Technology for a successful Remote Sensing/Precision Agriculture system is now in place, but other needed factors have been missing. Commercial satellite systems can now image the Earth (multi-spectrally) with a resolution as fine as 2.5 m. Precision variable dispensing systems using GPS are now available and affordable. Crop models that predict yield as a function of soil, chemical, and irrigation parameter levels have been developed. Personal computers and internet access are now in place in most farm homes and can provide a mechanism for periodically disseminating advice on what quantities of water and chemicals are needed in specific regions of each field. Several processes have been selected that fuse the disparate sources of information on the current and historic states of the crop and soil, and the remaining resource levels available, with the critical decisions that farmers are required to make. These are done in a way that is easy for the farmer to understand and profitable to implement. A "Constrained Optimization Algorithm" to further improve these processes will be presented. The objective function of the model will used to maximize the farmer's profit via increasing yields while decreasing environmental damage and decreasing applications of costly treatments. This model will incorporate information from Remote Sensing, from in-situ weather sources, from soil history, and from tacit farmer knowledge of the relative productivity of selected "Management Zones" of the farm, to provide incremental advice throughout the growing season on the optimum usage of water and chemical treatments.

Remote Sensing Forage Quality for Browsing Herbivores: A Case Study of Cutting Edge Koala Conservation

NASA Astrophysics Data System (ADS)

Youngentob, K. N.; Au, J.; Held, A. A.; Foley, W. J.; Possingham, H. P.

2014-12-01

Managing landscapes for conservation requires a capacity to measure habitat quality. Although multiple factors are often responsible for the distribution and abundance of herbivores, spatial variations in the quality and quantity of plant forage are known to be important for many species. While we cannot see the chemical complexity of landscapes with our naked-eye, advances in imaging spectroscopy are making it possible to assess the quality of forage on a landscape-scale. Much research in this area has focused on the ability to estimate foliar nitrogen (N), because N is believed to be a limiting nutrient for many leaf eating animals. However, the total quantity of foliar N does not necessarily reflect the amount of N that can be utilized by herbivores. Available nitrogen (AvailN) is an invitro measure of forage quality that integrates the influence of tannins and fibre on the amount of foliar N that is available for digestion by herbivores. This may be a more meaningful measure of forage quality than total N for the many herbivorous species that are sensitive to the effects of tannins. Our previous research has demonstrated that it is possible to estimate this integrated measure of foliar nutritional quality at an individual tree crown level across multiple tree species using imaging spectroscopy (HyMap). Here we present a case study of how this remote sensing data is being used to help inform landscape management and conservation decisions for an iconic Australian species, the koala (Phascolarctos cinereus). We review the methods involved in developing maps of integrated measures of foliar nutritional quality for browsing herbivores with airborne imaging spectroscopy data and discuss their applications for wildlife management.

Site-specific nutrient management systems

USDA-ARS?s Scientific Manuscript database

Site-specific nutrient management systems were created to manage for spatial and temporal variability in biophysical factors that determine the availability and demand of crop nutrients. These systems differ among geographical regions in the information utilized and way they operate to accomplish th...

Spatial Heterogeneity of Soil Nutrients after the Establishment of Caragana intermedia Plantation on Sand Dunes in Alpine Sandy Land of the Tibet Plateau

PubMed Central

Li, Qingxue; Jia, Zhiqing; Zhu, Yajuan; Wang, Yongsheng; Li, Hong; Yang, Defu; Zhao, Xuebin

2015-01-01

The Gonghe Basin region of the Tibet Plateau is severely affected by desertification. Compared with other desertified land, the main features of this region is windy, cold and short growing season, resulting in relatively difficult for vegetation restoration. In this harsh environment, identification the spatial distribution of soil nutrients and analysis its impact factors after vegetation establishment will be helpful for understanding the ecological relationship between soil and environment. Therefore, in this study, the 12-year-old C. intermedia plantation on sand dunes was selected as the experimental site. Soil samples were collected under and between shrubs on the windward slopes, dune tops and leeward slopes with different soil depth. Then analyzed soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), available nitrogen (AN), available phosphorus (AP) and available potassium (AK). The results showed that the spatial heterogeneity of soil nutrients was existed in C. intermedia plantation on sand dunes. (1) Depth was the most important impact factor, soil nutrients were decreased with greater soil depth. One of the possible reasons is that windblown fine materials and litters were accumulated on surface soil, when they were decomposed, more nutrients were aggregated on surface soil. (2) Topography also affected the distribution of soil nutrients, more soil nutrients distributed on windward slopes. The herbaceous coverage were higher and C. intermedia ground diameter were larger on windward slopes, both of them probably related to the high soil nutrients level for windward slopes. (3) Soil “fertile islands” were formed, and the “fertile islands” were more marked on lower soil nutrients level topography positions, while it decreased towards higher soil nutrients level topography positions. The enrichment ratio (E) for TN and AN were higher than other nutrients, most likely because C. intermedia is a leguminous shrub. PMID:25946170

Spatial Heterogeneity of Soil Nutrients after the Establishment of Caragana intermedia Plantation on Sand Dunes in Alpine Sandy Land of the Tibet Plateau.

PubMed

Li, Qingxue; Jia, Zhiqing; Zhu, Yajuan; Wang, Yongsheng; Li, Hong; Yang, Defu; Zhao, Xuebin

2015-01-01

The Gonghe Basin region of the Tibet Plateau is severely affected by desertification. Compared with other desertified land, the main features of this region is windy, cold and short growing season, resulting in relatively difficult for vegetation restoration. In this harsh environment, identification the spatial distribution of soil nutrients and analysis its impact factors after vegetation establishment will be helpful for understanding the ecological relationship between soil and environment. Therefore, in this study, the 12-year-old C. intermedia plantation on sand dunes was selected as the experimental site. Soil samples were collected under and between shrubs on the windward slopes, dune tops and leeward slopes with different soil depth. Then analyzed soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), available nitrogen (AN), available phosphorus (AP) and available potassium (AK). The results showed that the spatial heterogeneity of soil nutrients was existed in C. intermedia plantation on sand dunes. (1) Depth was the most important impact factor, soil nutrients were decreased with greater soil depth. One of the possible reasons is that windblown fine materials and litters were accumulated on surface soil, when they were decomposed, more nutrients were aggregated on surface soil. (2) Topography also affected the distribution of soil nutrients, more soil nutrients distributed on windward slopes. The herbaceous coverage were higher and C. intermedia ground diameter were larger on windward slopes, both of them probably related to the high soil nutrients level for windward slopes. (3) Soil "fertile islands" were formed, and the "fertile islands" were more marked on lower soil nutrients level topography positions, while it decreased towards higher soil nutrients level topography positions. The enrichment ratio (E) for TN and AN were higher than other nutrients, most likely because C. intermedia is a leguminous shrub.

Remote Sensing Marine Ecology: Wind-driven algal blooms in the open oceans and their ecological impacts

NASA Astrophysics Data System (ADS)

Tang, DanLing

2016-07-01

Algal bloom not only can increase the primary production but also could result in negative ecological consequence, e.g., Harmful Algal Blooms (HABs). According to the classic theory for the formation of algal blooms "critical depth" and "eutrophication", oligotrophic sea area is usually difficult to form a large area of algal blooms, and actually the traditional observation is only sporadic capture to the existence of algal blooms. Taking full advantage of multiple data of satellite remote sensing, this study: 1), introduces "Wind-driven algal blooms in open oceans: observation and mechanisms" It explained except classic coastal Ekman transport, the wind through a variety of mechanisms affecting the formation of algal blooms. Proposed a conceptual model of "Strong wind -upwelling-nutrient-phytoplankton blooms" in Western South China Sea (SCS) to assess role of wind-induced advection transport in phytoplankton bloom formation. It illustrates the nutrient resources that support long-term offshore phytoplankton blooms in the western SCS; 2), Proposal of the theory that "typhoons cause vertical mixing, induce phytoplankton blooms", and quantify their important contribution to marine primary production; Proposal a new ecological index for typhoon. Proposed remote sensing inversion models. 3), Finding of the spatial and temporaldistributions pattern of harmful algal bloom (HAB)and species variations of HAB in the South Yellow Sea and East China Sea, and in the Pearl River estuary, and their oceanic dynamic mechanisms related with monsoon; The project developed new techniques and generated new knowledge, which significantly improved understanding of the formation mechanisms of algal blooms. 1), It proposed "wind-pump" mechanism integrates theoretical system combing "ocean dynamics, development of algal blooms, and impact on primary production", which will benefit fisheries management. 2), A new interdisciplinary subject "Remote Sensing Marine Ecology"(RSME) has been developed via these achievements.

Fatal attraction: vegetation responses to nutrient inputs attract herbivores to infectious anthrax carcass sites

PubMed Central

Turner, Wendy C.; Kausrud, Kyrre L.; Krishnappa, Yathin S.; Cromsigt, Joris P. G. M.; Ganz, Holly H.; Mapaure, Isaac; Cloete, Claudine C.; Havarua, Zepee; Küsters, Martina; Getz, Wayne M.; Stenseth, Nils Chr.

2014-01-01

Parasites can shape the foraging behaviour of their hosts through cues indicating risk of infection. When cues for risk co-occur with desired traits such as forage quality, individuals face a trade-off between nutrient acquisition and parasite exposure. We evaluated how this trade-off may influence disease transmission in a 3-year experimental study of anthrax in a guild of mammalian herbivores in Etosha National Park, Namibia. At plains zebra (Equus quagga) carcass sites we assessed (i) carcass nutrient effects on soils and grasses, (ii) concentrations of Bacillus anthracis (BA) on grasses and in soils, and (iii) herbivore grazing behaviour, compared with control sites, using motion-sensing camera traps. We found that carcass-mediated nutrient pulses improved soil and vegetation, and that BA is found on grasses up to 2 years after death. Host foraging responses to carcass sites shifted from avoidance to attraction, and ultimately to no preference, with the strength and duration of these behavioural responses varying among herbivore species. Our results demonstrate that animal carcasses alter the environment and attract grazing hosts to parasite aggregations. This attraction may enhance transmission rates, suggesting that hosts are limited in their ability to trade off nutrient intake with parasite avoidance when relying on indirect cues. PMID:25274365

Long-Term Time Series of Remote Sensing Observations for Development of Regulatory Water Quality Standards

NASA Technical Reports Server (NTRS)

Blonski, Slawomir; Spiering, Bruce A.; Holekamp, Kara L.

2010-01-01

Water quality standards in the U.S. consist of: designated uses (the services that a water body provides; e.g., drinking water, aquatic life, harvestable species, recreation) . criteria that define the environmental conditions that must be maintained to support the uses For estuaries and coastal waters in the Gulf of Mexico, there are no numeric (quantitative) criteria to protect designated uses from effects of nutrients. This is largely due to the absence of adequate data that would quantitatively link biological conditions to nutrient concentrations. The Gulf of Mexico Alliance, an organization fostering collaboration between the Gulf States and U.S. Federal agencies, has identified the development of the numeric nutrient criteria as a major step leading to reduction in MODIS Products Figure 6. Map of the Mobile Bay with a yellow patch indicating the Bon Secour Bay area selected in this study for averaging water clarity parameters retrieved from MODIS datasets. nutrient inputs to coastal ecosystems. Nutrient enrichment in estuaries and coastal waters can be quantified based on response variables that measure phytoplankton biomass and water clarity. Long-term, spatially and temporally resolved measurements of chlorophyll a concentration, total concentration of suspended solids, and water clarity are needed to establish reference conditions and to quantify stressor-response relationships.

Significance of Plankton Community Structure and Nutrient Availability for the Control of Dinoflagellate Blooms by Parasites: A Modeling Approach

PubMed Central

Alves-de-Souza, Catharina; Pecqueur, David; Le Floc’h, Emilie; Mas, Sébastien; Roques, Cécile; Mostajir, Behzad; Vidussi, Franscesca; Velo-Suárez, Lourdes; Sourisseau, Marc; Fouilland, Eric; Guillou, Laure

2015-01-01

Dinoflagellate blooms are frequently observed under temporary eutrophication of coastal waters after heavy rains. Growth of these opportunistic microalgae is believed to be promoted by sudden input of nutrients and the absence or inefficiency of their natural enemies, such as grazers and parasites. Here, numerical simulations indicate that increasing nutrient availability not only promotes the formation of dinoflagellate blooms but can also stimulate their control by protozoan parasites. Moreover, high abundance of phytoplankton other than dinoflagellate hosts might have a significant dilution effect on the control of dinoflagellate blooms by parasites, either by resource competition with dinoflagellates (thus limiting the number of hosts available for infection) or by affecting numerical-functional responses of grazers that consume free-living parasite stages. These outcomes indicate that although both dinoflagellates and their protozoan parasites are directly affected by nutrient availability, the efficacy of the parasitic control of dinoflagellate blooms under temporary eutrophication depends strongly on the structure of the plankton community as a whole. PMID:26030411

Parasite and nutrient enrichment effects on Daphnia interspecific competition.

PubMed

Decaestecker, Ellen; Verreydt, Dino; De Meester, Luc; Declerck, Steven A J

2015-05-01

Increased productivity due to nutrient enrichment is hypothesized to affect density-dependent processes, such as transmission success of horizontally transmitting parasites. Changes in nutrient availability can also modify the stoichiometry and condition of individual hosts, which may affect their susceptibility for parasites as well as the growth conditions for parasites within the host. Consequently, if not balanced by increased host immuno-competence or life history responses, changes in the magnitude of parasite effects with increasing nutrient availability are expected. If these parasite effects are host-species specific, this may lead to shifts in the host community structure. We here used the Daphnia- parasite model system to study the effect of nutrient enrichment on parasite-mediated competition in experimental mesocosms. In the absence of parasites, D. magna was competitively dominant to D. pulex at both low and high nutrient levels. Introduction of parasites resulted in infections of D. magna, but not of D. pulex and, as such, reversed the competitive hierarchy between these two species. Nutrient addition resulted in an increased prevalence and infection intensity of some of the parasites on D. magna. However, there was no evidence that high nutrient levels enhanced negative effects of parasites on the hosts. Costs associated with parasite infections may have been compensated by better growth conditions for D. magna in the presence of high nutrient levels.

A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis.

PubMed

Li, Legong; Kim, Beom-Gi; Cheong, Yong Hwa; Pandey, Girdhar K; Luan, Sheng

2006-08-15

Nutrient sensing is critical for plant adaptation to the environment. Because of extensive farming and erosion, low content of mineral nutrients such as potassium (K(+)) in soils becomes a limiting factor for plant growth. In response to low-K conditions, plants enhance their capability of K(+) uptake through an unknown signaling mechanism. Here we report the identification of a Ca(2+)-dependent pathway for low-K response in Arabidopsis. We are not aware of any other example of a molecular pathway for a nutrient response in plants. Earlier genetic analyses revealed three genes encoding two Ca(2+) sensors (CBL1 and CBL9) and their target protein kinase (CIPK23) to be critical for plant growth on low-K media and for stomatal regulation, indicating that these calcium signaling components participate in the low-K response and turgor regulation. In this study, we show that the protein kinase CIPK23 interacted with, and phosphorylated, a voltage-gated inward K(+) channel (AKT1) required for K(+) acquisition in Arabidopsis. In the Xenopus oocyte system, our studies showed that interacting calcium sensors (CBL1 and CBL9) together with target kinase CIPK23, but not either component alone, activated the AKT1 channel in a Ca(2+)-dependent manner, connecting the Ca(2+) signal to enhanced K(+) uptake through activation of a K(+) channel. Disruption of both CBL1 and CBL9 or CIPK23 gene in Arabidopsis reduced the AKT1 activity in the mutant roots, confirming that the Ca(2+)-CBL-CIPK pathway functions to orchestrate transporting activities in planta according to external K(+) availability.

Nutrient concentrations and fluxes in tributaries to the Swan-Canning estuary, Western Australia

USGS Publications Warehouse

Peters, N.E.; Donohue, R.

1999-01-01

In Western Australia, catchment nutrient availability on an areal basis is primarily controlled by the disposal of animal waste and the type and rate of fertilizer application, particularly in coastal areas. The coastal areas receive notably higher rainfall and have more intense horticulture and animal production than inland areas, and are undergoing rapid urbanization, particularly adjacent to the estuary. Also, the surficial aquifers on the coastal plain are generally sandy having a low nutrient retention capacity and rapidly transmit soluble and colloidal material through the subsurface. In the Swan-Canning basin, high air and soil temperatures and seasonally arid conditions cause rapid mineralization of nitrogen and phosphorus. The nutrients are subsequently available for transport during the onset of seasonal wet weather, which typically begins during the period from late April to June. In addition to the rapid mobility of nutrients in streamwater from agricultural areas during the wet season, drains in urban areas, which typically have high nutrient concentrations, also are an important source of nutrients as the drains flow directly to the estuary throughout the year.

Community temporal variability increases with fluctuating resource availability

PubMed Central

Li, Wei; Stevens, M. Henry H.

2017-01-01

An increase in the quantity of available resources is known to affect temporal variability of aggregate community properties. However, it is unclear how might fluctuations in resource availability alter community-level temporal variability. Here we conduct a microcosm experiment with laboratory protist community subjected to manipulated resource pulses that vary in intensity, duration and time of supply, and examine the impact of fluctuating resource availability on temporal variability of the recipient community. The results showed that the temporal variation of total protist abundance increased with the magnitude of resource pulses, as protist community receiving infrequent resource pulses (i.e., high-magnitude nutrients per pulse) was relatively more unstable than community receiving multiple resource pulses (i.e., low-magnitude nutrients per pulse), although the same total amounts of nutrients were added to each community. Meanwhile, the timing effect of fluctuating resources did not significantly alter community temporal variability. Further analysis showed that fluctuating resource availability increased community temporal variability by increasing the degree of community-wide species synchrony and decreasing the stabilizing effects of dominant species. Hence, the importance of fluctuating resource availability in influencing community stability and the regulatory mechanisms merit more attention, especially when global ecosystems are experiencing high rates of anthropogenic nutrient inputs. PMID:28345592

Community temporal variability increases with fluctuating resource availability

NASA Astrophysics Data System (ADS)

Li, Wei; Stevens, M. Henry H.

2017-03-01

An increase in the quantity of available resources is known to affect temporal variability of aggregate community properties. However, it is unclear how might fluctuations in resource availability alter community-level temporal variability. Here we conduct a microcosm experiment with laboratory protist community subjected to manipulated resource pulses that vary in intensity, duration and time of supply, and examine the impact of fluctuating resource availability on temporal variability of the recipient community. The results showed that the temporal variation of total protist abundance increased with the magnitude of resource pulses, as protist community receiving infrequent resource pulses (i.e., high-magnitude nutrients per pulse) was relatively more unstable than community receiving multiple resource pulses (i.e., low-magnitude nutrients per pulse), although the same total amounts of nutrients were added to each community. Meanwhile, the timing effect of fluctuating resources did not significantly alter community temporal variability. Further analysis showed that fluctuating resource availability increased community temporal variability by increasing the degree of community-wide species synchrony and decreasing the stabilizing effects of dominant species. Hence, the importance of fluctuating resource availability in influencing community stability and the regulatory mechanisms merit more attention, especially when global ecosystems are experiencing high rates of anthropogenic nutrient inputs.

Vitamin B1 content in potato: effect of genotype, tuber enlargement, and storage, and estimation of stability and broad-sense heritability

USDA-ARS?s Scientific Manuscript database

Thiamine pyrophosphate (vitamin B1) is an essential nutrient in the human diet, and is often referred as the energy vitamin. Potato contains modest amounts of thiamine. However, genetic variation of thiamine concentrations in potato has never been investigated. In this study, we determined thiamine ...

Hydrography and biogeochemistry dedicated to the Mediterranean BGC-Argo network during a cruise with RV Tethys 2 in May 2015

NASA Astrophysics Data System (ADS)

Taillandier, Vincent; Wagener, Thibaut; D'Ortenzio, Fabrizio; Mayot, Nicolas; Legoff, Hervé; Ras, Joséphine; Coppola, Laurent; Pasqueron de Fommervault, Orens; Schmechtig, Catherine; Diamond, Emilie; Bittig, Henry; Lefevre, Dominique; Leymarie, Edouard; Poteau, Antoine; Prieur, Louis

2018-03-01

We report on data from an oceanographic cruise, covering western, central and eastern parts of the Mediterranean Sea, on the French research vessel Tethys 2 in May 2015. This cruise was fully dedicated to the maintenance and the metrological verification of a biogeochemical observing system based on a fleet of BGC-Argo floats. During the cruise, a comprehensive data set of parameters sensed by the autonomous network was collected. The measurements include ocean currents, seawater salinity and temperature, and concentrations of inorganic nutrients, dissolved oxygen and chlorophyll pigments. The analytical protocols and data processing methods are detailed, together with a first assessment of the calibration state for all the sensors deployed during the cruise. Data collected at stations are available at https://doi.org/10.17882/51678 and data collected along the ship track are available at https://doi.org/10.17882/51691.

ENSO-driven nutrient variability recorded by central equatorial Pacific corals

NASA Astrophysics Data System (ADS)

LaVigne, M.; Nurhati, I. S.; Cobb, K. M.; McGregor, H. V.; Sinclair, D. J.; Sherrell, R. M.

2012-12-01

Recent evidence for shifts in global ocean primary productivity suggests that surface ocean nutrient availability is a key link between global climate and ocean carbon cycling. Time-series records from satellite, in situ buoy sensors, and bottle sampling have documented the impact of the El Niño Southern Oscillation (ENSO) on equatorial Pacific hydrography and broad changes in biogeochemistry since the late 1990's, however, data are sparse prior to this. Here we use a new paleoceanographic nutrient proxy, coral P/Ca, to explore the impact of ENSO on nutrient availability in the central equatorial Pacific at higher-resolution than available from in situ nutrient data. Corals from Christmas (157°W 2°N) and Fanning (159°W 4°N) Islands recorded a well-documented decrease in equatorial upwelling as a ~40% decrease in P/Ca during the 1997-98 ENSO cycle, validating the application of this proxy to Pacific Porites corals. We compare the biogeochemical shifts observed through the 1997-98 event with two pre-TOGA-TAO ENSO cycles (1982-83 and 1986-87) reconstructed from a longer Christmas Island core. All three corals revealed ~30-40% P/Ca depletions during ENSO warming as a result of decreased regional wind stress, thermocline depth, and equatorial upwelling velocity. However, at the termination of each El Niño event, surface nutrients did not return to pre-ENSO levels for ~4-12 months after, SST as a result of increased biological draw down of surface nutrients. These records demonstrate the utility of high-resolution coral nutrient archives for understanding the impact of tropical Pacific climate on the nutrient and carbon cycling of this key region.

Larval outbreaks in West Greenland: Instant and subsequent effects on tundra ecosystem productivity and CO2 exchange.

PubMed

Lund, Magnus; Raundrup, Katrine; Westergaard-Nielsen, Andreas; López-Blanco, Efrén; Nymand, Josephine; Aastrup, Peter

2017-02-01

Insect outbreaks can have important consequences for tundra ecosystems. In this study, we synthesise available information on outbreaks of larvae of the noctuid moth Eurois occulta in Greenland. Based on an extensive dataset from a monitoring programme in Kobbefjord, West Greenland, we demonstrate effects of a larval outbreak in 2011 on vegetation productivity and CO 2 exchange. We estimate a decreased carbon (C) sink strength in the order of 118-143 g C m -2 , corresponding to 1210-1470 tonnes C at the Kobbefjord catchment scale. The decreased C sink was, however, counteracted the following years by increased primary production, probably facilitated by the larval outbreak increasing nutrient turnover rates. Furthermore, we demonstrate for the first time in tundra ecosystems, the potential for using remote sensing to detect and map insect outbreak events.

Soil moisture and biogeochemical factors influence the distribution of annual Bromus species

USGS Publications Warehouse

Belnap, Jayne; Stark, John Thomas; Rau, Benjamin; Allen, Edith B.; Phillips, Sue

2016-01-01

Abiotic factors have a strong influence on where annual Bromus species are found. At the large regional scale, temperature and precipitation extremes determine the boundaries of Bromusoccurrence. At the more local scale, soil characteristics and climate influence distribution, cover, and performance. In hot, dry, summer-rainfall-dominated deserts (Sonoran, Chihuahuan), little or noBromus is found, likely due to timing or amount of soil moisture relative to Bromus phenology. In hot, winter-rainfall-dominated deserts (parts of the Mojave Desert), Bromus rubens is widespread and correlated with high phosphorus availability. It also responds positively to additions of nitrogen alone or with phosphorus. On the Colorado Plateau, with higher soil moisture availability, factors limiting Bromus tectorum populations vary with life stage: phosphorus and water limit germination, potassium and the potassium/magnesium ratio affect winter performance, and water and potassium/magnesium affect spring performance. Controlling nutrients also change with elevation. In cooler deserts with winter precipitation (Great Basin, Columbia Plateau) and thus even greater soil moisture availability, B. tectorum populations are controlled by nitrogen, phosphorus, or potassium. Experimental nitrogen additions stimulate Bromus performance. The reason for different nutrients limiting in dissimilar climatic regions is not known, but it is likely that site conditions such as soil texture (as it affects water and nutrient availability), organic matter, and/or chemistry interact in a manner that regulates nutrient availability and limitations. Under future drier, hotter conditions,Bromus distribution is likely to change due to changes in the interaction between moisture and nutrient availability.

[Relationships between soil nutrients and rhizospheric soil microbial communities and enzyme activities in a maize-capsicum intercropping system].

PubMed

Xu, Qiang; Cheng, Zhi-Hui; Meng, Huan-Wen; Zhang, Yu

2007-12-01

By using plastic sheet and nylon mesh to partition the root systems of maize and capsicum in a maize-capsicum intercropping system, this paper studied the relationships between soil biological factors and nutritive status in the intercropping system, with no partitioning and maize monoculture and capsicum monoculture as the control. The results showed that intercropping maize and capsicum had its high superiority. In the treatments of no partitioning and nylon mesh portioning in the intercropping system, soil enzyme activities, microbial individuals and nutrient contents were significantly higher, compared with those in the treatments of nylon mesh partitioning and monocultures. All kinds of soil available nutrients showed significant or very significant positive correlations with soil biological factors, except that soil available Mg was negatively correlated with soil fungi and catalase activity. Pathway analysis indicated that in the intercropping system, soil urease, catalase, protease, and bacteria were the main factors affecting the accumulation of soil organic matter, saccharase was the most important factor affecting soil alkali-hydrolyzable N, urease was the most important factor affecting soil available P, and bacteria largely determined soil available K. Soil alkaline phosphatase and fungi selectively affected the accumulation of soil organic matter and available N, P and K. There was a slight negative correlation between soil actinomycetes and soil nutrients, suggesting that actinomycetes had little effect on soil nutrient formation.

Remote sensing aides studies of climate and wildlife in the Arctic-on land, at sea, and in the air (Invited)

NASA Astrophysics Data System (ADS)

Douglas, D. C.; Durner, G. M.; Gill, R. E.; Griffith, B.; Schmutz, J. A.

2013-12-01

Every day a variety of remote sensing technologies collects large volumes of data that are supporting new analyses and new interpretations about how weather and climate influence the status and distribution of wildlife populations worldwide. Understanding how climate presently affects wildlife is crucial for projecting how climate change could affect wildlife in the future. This talk highlights climate-related wildlife studies by the US Geological Survey in the Arctic. The Arctic is experiencing some of the most pronounced climate changes on earth, raising concerns for species that have evolved seasonal migration strategies tuned to habitat availability and quality. On land, large herbivores such as caribou select concentrated calving areas with high abundance of rapidly growing vegetation and calf survival increases with earlier green-up and with the quantity of food available to cows at peak lactation. Geese time their migrations and reproductive efforts to coincide with optimal plant phenology and peak nutrient availability and departures from this synchrony can influence the survival of goslings. At sea, the habitats of polar bears and other sea-ice-dependent species have dramatically changed over just the past two decades. The ice pack is comprised of younger ice that melts much more extensively during summer-a trend projected to continue by all general circulation models under all but the most aggressive greenhouse gas mitigation scenarios. Studies show that by mid-century optimal polar bear habitats will be so reduced that the species may become extirpated from some regions of the Arctic. In the air, a variety of shorebird species make non-stop endurance flights between northern and southern hemispheres. The bar-tailed godwit undertakes a trans-Pacific flight between Alaska and Australasia that lasts more than seven days and spans more than 10,000 km. Studies show that godwits time their flights to coincide with favorable wind conditions, but stochastic weather events en route can impose energetic costs that affect reproduction and possibly survival. As more is learned about how climate and climate change affect species and ecosystems, better adaptive management decisions can be made. Remote sensing will continue to play an essential role.

Developmental Strategy For Effective Sampling To Detect Possible Nutrient Fluxes In Oligotrophic Coastal Reef Waters In The Caribbean

NASA Astrophysics Data System (ADS)

Mendoza, W. G.; Corredor, J. E.; Ko, D.; Zika, R. G.; Mooers, C. N.

2008-05-01

The increasing effort to develop the coastal ocean observing system (COOS) in various institutions has gained momentum due to its high value to climate, environmental, economic, and health issues. The stress contributed by nutrients to the coral reef ecosystem is among many problems that are targeted to be resolved using this system. Traditional nutrient sampling has been inadequate to resolve issues on episodic nutrient fluxes in reef regions due to temporal and spatial variability. This paper illustrates sampling strategy using the COOS information to identify areas that need critical investigation. The area investigated is within the Puerto Rico subdomain (60-70oW, 15-20oN), and Caribbean Time Series (CaTS), World Ocean Circulation Experiment (WOCE), Intra-America Sea (IAS) ocean nowcast/forecast system (IASNFS), and other COOS-related online datasets are utilized. Nutrient profile results indicate nitrate is undetectable in the upper 50 m apparently due to high biological consumption. Nutrients are delivered in Puerto Rico particularly in the CaTS station either via a meridional jet formed from opposing cyclonic and anticyclonic eddies or wind-driven upwelling. The strong vertical fluctuation in the upper 50 m demonstrates a high anomaly in temperature and salinity and a strong cross correlation signal. High chlorophyll a concentration corresponding to seasonal high nutrient influx coincides with higher precipitation accumulation rates and apparent riverine input from the Amazon and Orinoco Rivers during summer (August) than during winter (February) seasons. Non-detectability of nutrients in the upper 50 m is a reflection of poor sampling frequency or the absence of a highly sensitive nutrient analysis method to capture episodic events. Thus, this paper was able to determine the range of depths and concentrations that need to be critically investigated to determine nutrient fluxes, nutrient sources, and climatological factors that can affect nutrient delivery. It also provides some insight into needed sampling rates and temporal and spatial domain choices. Finally, it demonstrates a scientific reconnaissance for a field study that is now possible with online in-situ and remote sensing observations and numerical simulations, as a consequence of IOOS in general and COOS in particular.

Native Mussels Alter Nutrient Availability and Reduce Blue-Green Algae Abundance

EPA Science Inventory

Nutrient cycling is a key process that ties all organisms together. This is especially apparent in stream environments in which nutrients are taken up readily and cycled through the system in a downstream trajectory. Ecological stoichiometry predicts that biogeochemical cycles of...

NUTRIENT TRANSPORT DURING BIOREMEDIATION OF CONTAMINATED BEACHES: EVALUATION WITH LITHIUM AS A CONSERVATIVE TRACER

EPA Science Inventory

Bioremediation of oil-contaminated beaches typically involves fertilization with nutrients that are thought to limit the growth rate of hydrocarbon-degrading bacteria. Much of the available technology involves application of fertilizers that release nutrients in a water-soluble ...

Sewage input reduces the consumption of Rhizophora mangle propagules by crabs in a subtropical mangrove system.

PubMed

Boehm, Frederike Ricarda; Sandrini-Neto, Leonardo; Moens, Tom; da Cunha Lana, Paulo

2016-12-01

Mangrove forests are highly productive and play a major role in global carbon cycling. Their carbon accumulation can be influenced through the consumption of nutrient-poor leaves and propagules by herbivore crabs. Anthropogenic nutrient input from sewage contamination is widespread in these often naturally nutrient-limited ecosystems. We hypothesised that sewage-mediated nutrient input to mangrove stands of Paranaguá Bay (southern Brazil), would alter the nutrient sources available for crabs, e.g. through microphytobenthos increase, and that this would reflect in their feeding behaviour. We predicted that propagules of Rhizophora mangle in contaminated stands would experience lower grazing pressure from their two main local consumers (Ucides cordatus and Goniopsis cruentata). We compared herbivory rates on R. mangle propagules in sewage contaminated and uncontaminated mangrove stands. We found that herbivory rates were significantly lower in contaminated than uncontaminated forests, but this pattern could not be clearly attributed to increased nutrient availability. Copyright © 2016 Elsevier Ltd. All rights reserved.

Metabolic effects of portal vein sensing.

PubMed

Mithieux, G

2014-09-01

The extrinsic gastrointestinal nerves are crucial in the sensing of nutrients and hormones and its translation in terms of control of food intake. Major macronutrients like glucose and protein are sensed by the extrinsic nerves located in the portal vein walls, which signal to the brain and account for the satiety phenomenon they promote. Glucose is sensed in the portal vein by neurons expressing the glucose receptor SGLT3, which activate the main regions of the brain involved in the control of food intake. Proteins indirectly act on food intake by inducing intestinal gluconeogenesis and its sensing by the portal glucose sensor. The mechanism involves a prior antagonism by peptides of the μ-opioid receptors present in the portal vein nervous system and a reflex arc with the brain inducing intestinal gluconeogenesis. In a comparable manner, short-chain fatty acids produced from soluble fibre act via intestinal gluconeogenesis to exert anti-obesity and anti-diabetic effects. In the case of propionate, the mechanism involves a prior activation of the free fatty acid receptor FFAR3 present in the portal nerves and a reflex arc initiating intestinal gluconeogenesis. © 2014 John Wiley & Sons Ltd.

The influence of microtopography on soil nutrients in created mitigation wetlands

USGS Publications Warehouse

Moser, K.F.; Ahn, C.; Noe, G.B.

2009-01-01

This study explores the relationship between microtopography and soil nutrients (and trace elements), comparing results for created and reference wetlands in Virginia, and examining the effects of disking during wetland creation. Replicate multiscale tangentially conjoined circular transects were used to quantify microtopography both in terms of elevation and by two microtopographic indices. Corresponding soil samples were analyzed for moisture content, total C and N, KCl-extractable NH4-N and NO3-N, and Mehlich-3 extractable P, Ca, Mg, K, Al, Fe, and Mn. Means and variances of soil nutrient/element concentrations were compared between created and natural wetlands and between disked and nondisked created wetlands. Natural sites had higher and more variable soil moisture, higher extractable P and Fe, lower Mn than created wetlands, and comparatively high variability in nutrient concentrations. Disked sites had higher soil moisture, NH4-N, Fe, and Mn than did nondisked sites. Consistently low variances (Levene test for inequality) suggested that nondisked sites had minimal nutrient heterogeneity. Across sites, low P availability was inferred by the molar ratio (Mehlich-3 [P/(Al + Fe)] < 0.06); strong intercorrelations among total C, total N, and extractable Fe, Al, and P suggested that humic-metal-P complexes may be important for P retention and availability. Correlations between nutrient/element concentrations and microtopographic indices suggested increased Mn and decreased K and Al availability with increased surface roughness. Disking appears to enhance water and nutrient retention, as well as nutrient heterogeneity otherwise absent from created wetlands, thus potentially promoting ecosystem development. ?? 2008 Society for Ecological Restoration International.

Systemic control of brown fat thermogenesis: integration of peripheral and central signals.

PubMed

Schulz, Tim J; Tseng, Yu-Hua

2013-10-01

Brown adipose tissue (BAT) is of great scientific interest as a potential target to treat obesity. The development of novel strategies to quantify brown fat thermogenesis in adult humans now enables minimally invasive assessment of novel pharmacotherapeutics. Input from the central nervous system via sympathetic efferents is widely regarded as the key controller of BAT-mediated thermogenesis in response to changes in body temperature or nutrient availability. More recently, however, it has become clear that locally secreted signals and endocrine factors originating from multiple organs can control the recruitment of brown adipocytes and, more importantly, induce thermogenesis in brown fat. Thus, they provide an attractive strategy to fine-tune brown fat thermogenesis independent of classical temperature sensing. Here, we summarize recent findings on bone morphogenetic protein signaling as an example of secreted factors in the regulation of brown adipocyte formation and systemic control of energy metabolism. We further highlight endocrine communication routes between the different types of brown adipocytes and other organs that contribute to regulation of thermogenesis. Thus, emerging evidence suggests that the classical mechanisms of central temperature sensing and sympathetic nervous system-driven thermogenesis are complemented by local and endocrine signals to determine systemic energy homeostasis. © 2013 New York Academy of Sciences.

A Study on Land Suitability for Rice Cultivation in Khordha District of Odisha (India) Using Remote Sensing and GIS

NASA Astrophysics Data System (ADS)

Rath, Sudhansu S.; Panda, Jagabandhu; Annadurai, R.; Nanda, Sachikanta

2018-05-01

With the global population on the rise, it is important to address the increasing demand for food. According to FAO (Food and Agriculture Organization, United Nations), by 2050, the developing countries must double their food production to meet the growing demand. Proper land utilization can be one of the solutions for this problem. In view of this, the current study focussed on land suitability analysis for Khordha district of Odisha (India) for rice crop. This study estimated that the amount of land suitable for rice cropping was 195,731 ha against the currently cultivated land of 122,183.38 ha. Therefore, there was a possibility of more amount of land that could be available for rice cultivation in Khordha district than the currently cultivated area. In order to perform this exercise, the land use and land cover data from IRS (Indian remote sensing satellite), soil nutrient parameters like pH values and nitrogen, potassium, phosphorous and organic carbon contents were considered. In addition, the climatic parameters such as near surface temperature, rainfall and number of rainy days were taken into account. The unused land identified in Khordha district in this study might be utilized for cultivating rice crop in this region.

A Study on Land Suitability for Rice Cultivation in Khordha District of Odisha (India) Using Remote Sensing and GIS

NASA Astrophysics Data System (ADS)

Rath, Sudhansu S.; Panda, Jagabandhu; Annadurai, R.; Nanda, Sachikanta

2018-02-01

With the global population on the rise, it is important to address the increasing demand for food. According to FAO (Food and Agriculture Organization, United Nations), by 2050, the developing countries must double their food production to meet the growing demand. Proper land utilization can be one of the solutions for this problem. In view of this, the current study focussed on land suitability analysis for Khordha district of Odisha (India) for rice crop. This study estimated that the amount of land suitable for rice cropping was 195,731 ha against the currently cultivated land of 122,183.38 ha. Therefore, there was a possibility of more amount of land that could be available for rice cultivation in Khordha district than the currently cultivated area. In order to perform this exercise, the land use and land cover data from IRS (Indian remote sensing satellite), soil nutrient parameters like pH values and nitrogen, potassium, phosphorous and organic carbon contents were considered. In addition, the climatic parameters such as near surface temperature, rainfall and number of rainy days were taken into account. The unused land identified in Khordha district in this study might be utilized for cultivating rice crop in this region.

Evidence for nonuniform permafrost degradation after fire in boreal landscapes

USGS Publications Warehouse

Minsley, Burke J.; Pastick, Neal J.; Wylie, Bruce K.; Brown, Dana R.N.; Kass, M. Andy

2016-01-01

Fire can be a significant driver of permafrost change in boreal landscapes, altering the availability of soil carbon and nutrients that have important implications for future climate and ecological succession. However, not all landscapes are equally susceptible to fire-induced change. As fire frequency is expected to increase in the high latitudes, methods to understand the vulnerability and resilience of different landscapes to permafrost degradation are needed. We present a combination of multiscale remote sensing, geophysical, and field observations that reveal details of both near-surface (<1 m) and deeper (>1 m) impacts of fire on permafrost. Along 11 transects that span burned-unburned boundaries in different landscape settings within interior Alaska, subsurface electrical resistivity and nuclear magnetic resonance data indicate locations where permafrost appears to be resilient to disturbance from fire, areas where warm permafrost conditions exist that may be most vulnerable to future change, and also areas where permafrost has thawed. High-resolution geophysical data corroborate remote sensing interpretations of near-surface permafrost and also add new high-fidelity details of spatial heterogeneity that extend from the shallow subsurface to depths of about 10 m. Results show that postfire impacts on permafrost can be variable and depend on multiple factors such as fire severity, soil texture, soil moisture, and time since fire.

The effect of herbicide application on rangeland soil nutrient availability

USDA-ARS?s Scientific Manuscript database

Very sparse literature exists on the effect of soil active herbicides on nutrient availability. As part of a larger rangeland rehabilitation project, on four sites in northern Nevada, we quantified the effect of the herbicides Landmark®, Perspective®, and Plateau® relative to controls on surface soi...

Nutrient source and tillage influences on nitrogen availability in a Southern Piedmont corn cropping system

USDA-ARS?s Scientific Manuscript database

Combinations of conservation tillage and poultry litter (PL) can increase crop production in southeastern USA soils compared to conventional tillage (CT) and chemical fertilizer (CF). The reason for the beneficial response is usually attributed to improved water and nutrient availability. We evaluat...

Effects of Phos-Chek® on soil nutrient availability

USDA-ARS?s Scientific Manuscript database

Wildfire frequencies and intensities have been steadily increasing on western US landscapes. Phos-chek® is an aerially-applied fire retardant used to contain and control wildfires. Composed of ammonium and phosphate salts, Phos-chek® has the potential to increase soil nutrient availability of N and ...

Effect of temperature and nutrients on the growth and development of seedlings of an invasive plant.

PubMed

Skálová, Hana; Moravcová, Lenka; Dixon, Anthony F G; Kindlmann, P; Pyšek, Petr

2015-04-28

Plant species distributions are determined by the response of populations to regional climates; however, little is known about how alien plants that arrive in central Europe from climatically warmer regions cope with the temperature conditions at the early stage of population development. Ambrosia artemisiifolia (common ragweed) is an invasive annual plant causing considerable health and economic problems in Europe. Although climate-based models predict that the whole of the Czech Republic is climatically suitable for this species, it is confined to the warmest regions. To determine the factors possibly responsible for its restricted occurrence, we investigated the effects of temperature and nutrient availability on its seedlings. The plants were cultivated at one of seven temperature regimes ranging from 10 to 34 °C, combined with three nutrient levels. The data on the rate of leaf development were used to calculate the lower developmental threshold (LDT, the temperature, in °C, below which development ceases), the sum of effective temperatures (SET, the amount of heat needed to complete a developmental stage measured in degree days above LDT) and width of the thermal window. The rate of development decreased with decrease in temperature and nutrient supply. Besides this, the decrease in the availability of nutrients resulted in decreased LDT, increased SET and wider thermal window. The dependence of LDT and SET on the availability of nutrients contradicts the concept that thermal constants do not vary. Our results highlight temperature as the main determinant of common ragweed's distribution and identify nutrient availability as a factor that results in the realized niche being smaller than the fundamental niche; both of these need to be taken into account when predicting the future spread of A. artemisiifolia. Published by Oxford University Press on behalf of the Annals of Botany Company.

X-ray fluorescence spectrometry-based approach to precision management of bioavailable phosphorus in soil environments

USDA-ARS?s Scientific Manuscript database

Declining nutrient use efficiency in crop production has been a global priority to preserve high agricultural productivity with finite non-renewable nutrient resources, in particular phosphorus (P). Rapid spectroscopic methods increase measurement density of soil nutrients, and the availability of ...

Combined Influence of Landscape Composition and Nutrient Inputs on Lake Trophic Structure

EPA Science Inventory

The concentration of chlorophyll a is a measure of the biological productivity of a lake and is largely (but not exclusively) determined by available nutrients. As nutrient inputs increase, productivity increases and lakes transition from low trophic state (e.g. oligotrophic) to...

Measuring nutrient flux in Pacific Coast salt marshes using fluctuating water-level chambers

EPA Science Inventory

Nutrient removal from the water column is an important ecosystem function that contributes to the production of clean water, a final valued ecosystem service of wetlands. However, little data is currently available for nutrient exchange in Pacific Northwest tidal ecosystems. We h...

Freshwater bacterioplankton richness in oligotrophic lakes depends on nutrient availability rather than on species–area relationships

PubMed Central

Logue, Jürg Brendan; Langenheder, Silke; Andersson, Anders F; Bertilsson, Stefan; Drakare, Stina; Lanzén, Anders; Lindström, Eva S

2012-01-01

A central goal in ecology is to grasp the mechanisms that underlie and maintain biodiversity and patterns in its spatial distribution can provide clues about those mechanisms. Here, we investigated what might determine the bacterioplankton richness (BR) in lakes by means of 454 pyrosequencing of the 16S rRNA gene. We further provide a BR estimate based upon a sampling depth and accuracy, which, to our knowledge, are unsurpassed for freshwater bacterioplankton communities. Our examination of 22 669 sequences per lake showed that freshwater BR in fourteen nutrient-poor lakes was positively influenced by nutrient availability. Our study is, thus, consistent with the finding that the supply of available nutrients is a major driver of species richness; a pattern that may well be universally valid to the world of both micro- and macro-organisms. We, furthermore, observed that BR increased with elevated landscape position, most likely as a consequence of differences in nutrient availability. Finally, BR decreased with increasing lake and catchment area that is negative species–area relationships (SARs) were recorded; a finding that re-opens the debate about whether positive SARs can indeed be found in the microbial world and whether positive SARs can in fact be pronounced as one of the few ‘laws' in ecology. PMID:22170419

Synoptic thermal and oceanographic parameter distributions in the New York Bight Apex

NASA Technical Reports Server (NTRS)

Johnson, R. W.; Bahn, G. S.; Thomas, J. P.

1981-01-01

Concurrent surface water measurements made from a moving oceanographic research vessel were used to calibrate and interpret remotely sensed data collected over a plume in the New York Bight Apex on 23 June 1977. Multiple regression techniques were used to develop equations to map synoptic distributions of chlorophyll a and total suspended matter in the remotely sensed scene. Thermal (which did not have surface calibration values) and water quality parameter distributions indicated a cold mass of water in the Bight Apex with an overflowing nutrient-rich warm water plume that originated in the Sandy Hook Bay and flowed south near the New Jersey shoreline. Data analysis indicates that remotely sensed data may be particularly useful for studying physical and biological processes in the top several metres of surface water at plume boundaries.

Effect of available nitrogen on phytoavailability and bioaccumulation of hexavalent and trivalent chromium in hankow willows (Salix matsudana Koidz).

PubMed

Yu, Xiao-Zhang; Gu, Ji-Dong

2008-06-01

The effect of available nitrogen in nutrient solution on removal of two chemical forms of chromium (Cr) by plants was investigated. Pre-rooted hankow willows (Salix matsudana Koidz) were grown in a hydroponic solution system with or without nitrogen, and amended with hexavalent chromium [Cr (VI)] or trivalent chromium [Cr (III)] at 25.0+/-0.5 degrees C for 192 h. The results revealed that higher removal of Cr by plants was achieved from the hydroponic solutions without any nitrogen than those containing nitrogen. Although faster removal of Cr (VI) than Cr (III) was observed, translocation of Cr (III) within plant materials was more efficient than Cr (VI). Substantial difference existed in the distribution of Cr in different parts of plant tissues due to the nitrogen in nutrient solutions (p<0.05): lower stems were the major sink for both Cr species in willows grown in the N-free nutrient solutions and more Cr was accumulated in the roots of plants in N-containing ones. No significant difference was found in the removal rate of Cr (VI) between willows grown in the N-free and N-containing solutions (p>0.05). Removal rates of Cr (III) decreased linearly with the strength of nutrient solutions with or without N addition (p<0.01). Translocation efficiencies of both Cr species increased proportionally with the strength of N-containing nutrient solutions and decreased with the strength of N-free nutrient solutions. Results suggest that uptake and translocation mechanisms of Cr (VI) and Cr (III) are apparently different in hankow willows. The presence of easily available nitrogen and other nutrient elements in the nutrient solutions had a more pronounced influence on the uptake of Cr (III) than Cr (VI). Nitrogen availability and quantities in the ambient environment will affect the translocation of both Cr species and their distribution in willows in phytoremediation.

The invasive species Alliaria petiolata (garlic mustard) increases soil nutrient availability in northern hardwood-conifer forests.

PubMed

Rodgers, Vikki L; Wolfe, Benjamin E; Werden, Leland K; Finzi, Adrien C

2008-09-01

The invasion of non-native plants can alter the diversity and activity of soil microorganisms and nutrient cycling within forests. We used field studies to analyze the impact of a successful invasive groundcover, Alliaria petiolata, on fungal diversity, soil nutrient availability, and pH in five northeastern US forests. We also used laboratory and greenhouse experiments to test three mechanisms by which A. petiolata may alter soil processes: (1) the release of volatile, cyanogenic glucosides from plant tissue; (2) the exudation of plant secondary compounds from roots; and (3) the decomposition of litter. Fungal community composition was significantly different between invaded and uninvaded soils at one site. Compared to uninvaded plots, plots invaded by A. petiolata were consistently and significantly higher in N, P, Ca and Mg availability, and soil pH. In the laboratory, the release of volatile compounds from the leaves of A. petiolata did not significantly alter soil N availability. Similarly, in the greenhouse, the colonization of native soils by A. petiolata roots did not alter soil nutrient cycling, implying that the exudation of secondary compounds has little effect on soil processes. In a leaf litter decomposition experiment, however, green rosette leaves of A. petiolata significantly increased the rate of decomposition of native tree species. The accelerated decomposition of leaf litter from native trees in the presence of A. petiolata rosette leaves shows that the death of these high-nutrient-content leaves stimulates decomposition to a greater extent than any negative effect that secondary compounds may have on the activity of the microbes decomposing the native litter. The results presented here, integrated with recent related studies, suggest that this invasive plant may change soil nutrient availability in such a way as to create a positive feedback between site occupancy and continued proliferation.

Comparison of methods for nutrient measurement in calcareous soils: Ion-exchange resin bag, capsule, membrane, and chemical extractions

USGS Publications Warehouse

Sherrod, S.K.; Belnap, J.; Miller, M.E.

2002-01-01

Four methods for measuring quantities of 12 plant-available nutrients were compared using three sandy soils in a series of three experiments. Three of the methods use different ion-exchange resin forms—bags, capsules, and membranes—and the fourth was conventional chemical extraction. The first experiment compared nutrient extraction data from a medium of sand saturated with a nutrient solution. The second and third experiments used Nakai and Sheppard series soils from Canyonlands National Park, which are relatively high in soil carbonates. The second experiment compared nutrient extraction data provided by the four methods from soils equilibrated at two temperatures, “warm” and “cold.” The third experiment extracted nutrients from the same soils in a field equilibration. Our results show that the four extraction techniques are not comparable. This conclusion is due to differences among the methods in the net quantities of nutrients extracted from equivalent soil volumes, in the proportional representation of nutrients within similar soils and treatments, in the measurement of nutrients that were added in known quantities, and even in the order of nutrients ranked by net abundance. We attribute the disparities in nutrient measurement among the different resin forms to interacting effects of the inherent differences in resin exchange capacity, differences among nutrients in their resin affinities, and possibly the relatively short equilibration time for laboratory trials. One constraint for measuring carbonate-related nutrients in high-carbonate soils is the conventional ammonium acetate extraction method, which we suspect of dissolving fine CaCO3 particles that are more abundant in Nakai series soils, resulting in erroneously high Ca2+ estimates. For study of plant-available nutrients, it is important to identify the nutrients of foremost interest and understand differences in their resin sorption dynamics to determine the most appropriate extraction method.

Gustatory and metabolic perception of nutrient stress in Drosophila.

PubMed

Linford, Nancy J; Ro, Jennifer; Chung, Brian Y; Pletcher, Scott D

2015-02-24

Sleep loss is an adaptive response to nutrient deprivation that alters behavior to maximize the chances of feeding before imminent death. Organisms must maintain systems for detecting the quality of the food source to resume healthy levels of sleep when the stress is alleviated. We determined that gustatory perception of sweetness is both necessary and sufficient to suppress starvation-induced sleep loss when animals encounter nutrient-poor food sources. We further find that blocking specific dopaminergic neurons phenocopies the absence of gustatory stimulation, suggesting a specific role for these neurons in transducing taste information to sleep centers in the brain. Finally, we show that gustatory perception is required for survival, specifically in a low nutrient environment. Overall, these results demonstrate an important role for gustatory perception when environmental food availability approaches zero and illustrate the interplay between sensory and metabolic perception of nutrient availability in regulating behavioral state.

Effects of Liming on Forage Availability and Nutrient Content in a Forest Impacted by Acid Rain

PubMed Central

Pabian, Sarah E.; Ermer, Nathan M.; Tzilkowski, Walter M.; Brittingham, Margaret C.

2012-01-01

Acidic deposition and subsequent forest soil acidification and nutrient depletion can affect negatively the growth, health and nutrient content of vegetation, potentially limiting the availability and nutrient content of forage for white-tailed deer (Odocoileus virginianus) and other forest herbivores. Liming is a mitigation technique that can be used to restore forest health in acidified areas, but little is known about how it affects the growth or nutrient content of deer forage. We examined the effects of dolomitic limestone application on the growth and chemical composition of understory plants in an acidified forest in central Pennsylvania, with a focus on vegetative groups included as white-tailed deer forage. We used a Before-After-Control-Impact study design with observations 1 year before liming and up to 5 years post-liming on 2 treated and 2 untreated 100-ha sites. Before liming, forage availability and several nutrients were below levels considered optimal for white-tailed deer, and many vegetative characteristics were related to soil chemistry. We observed a positive effect of liming on forb biomass, with a 2.7 fold increase on limed sites, but no biomass response in other vegetation groups. We observed positive effects of liming on calcium and magnesium content and negative effects on aluminum and manganese content of several plant groups. Responses to liming by forbs and plant nutrients show promise for improving vegetation health and forage quality and quantity for deer. PMID:22761890

Effects of liming on forage availability and nutrient content in a forest impacted by acid rain.

PubMed

Pabian, Sarah E; Ermer, Nathan M; Tzilkowski, Walter M; Brittingham, Margaret C

2012-01-01

Acidic deposition and subsequent forest soil acidification and nutrient depletion can affect negatively the growth, health and nutrient content of vegetation, potentially limiting the availability and nutrient content of forage for white-tailed deer (Odocoileus virginianus) and other forest herbivores. Liming is a mitigation technique that can be used to restore forest health in acidified areas, but little is known about how it affects the growth or nutrient content of deer forage. We examined the effects of dolomitic limestone application on the growth and chemical composition of understory plants in an acidified forest in central Pennsylvania, with a focus on vegetative groups included as white-tailed deer forage. We used a Before-After-Control-Impact study design with observations 1 year before liming and up to 5 years post-liming on 2 treated and 2 untreated 100-ha sites. Before liming, forage availability and several nutrients were below levels considered optimal for white-tailed deer, and many vegetative characteristics were related to soil chemistry. We observed a positive effect of liming on forb biomass, with a 2.7 fold increase on limed sites, but no biomass response in other vegetation groups. We observed positive effects of liming on calcium and magnesium content and negative effects on aluminum and manganese content of several plant groups. Responses to liming by forbs and plant nutrients show promise for improving vegetation health and forage quality and quantity for deer.

Bile acid receptors link nutrient sensing to metabolic regulation

PubMed Central

Li, Jibiao; Li, Tiangang

2017-01-01

Non-alcoholic fatty liver disease (NAFLD) is a common liver disease in Western populations. Non-alcoholic steatohepatitis (NASH) is a more debilitating form of NAFLD characterized by hepatocellular injury and inflammation, which significantly increase the risk of end-stage liver and cardiovascular diseases. Unfortunately, there are no available drug therapies for NASH. Bile acids are physiological detergent molecules that are synthesized from cholesterol exclusively in the hepatocytes. Bile acids circulate between the liver and intestine, where they are required for cholesterol solubilization in the bile and dietary fat emulsification in the gut. Bile acids also act as signaling molecules that regulate metabolic homeostasis and inflammatory processes. Many of these effects are mediated by the bile acid-activated nuclear receptor farnesoid X receptor (FXR) and the G protein-coupled receptor TGR5. Nutrient signaling regulates hepatic bile acid synthesis and circulating plasma bile acid concentrations, which in turn control metabolic homeostasis. The FXR agonist obeticholic acid has had beneficial effects on NASH in recent clinical trials. Preclinical studies have suggested that the TGR5 agonist and the FXR/TGR5 dual agonist are also potential therapies for metabolic liver diseases. Extensive studies in the past few decades have significantly improved our understanding of the metabolic regulatory function of bile acids, which has provided the molecular basis for developing promising bile acid-based therapeutic agents for NASH treatment. PMID:29098111

Fire as a Factor of Variation of Soil Respiration in Amazonia of Peru

NASA Astrophysics Data System (ADS)

Suarez, L.; Kruijt, B.

2007-05-01

Severe changes are affecting the role of Amazonia in the Earth system. One of these possible effects could be the modification of the relevance of soil in the carbon cycle. In this sense, fire is an important factor for mobilizing C from the soil to the atmosphere, mainly as CO2. This could have an important effect in the global warming. Our proposal will evaluate the variation of the soil respiration related to the seasonality and the fire effects on soils in the Amazonia of Peru and Brasil. In experimental parcels of four locations of Peru with different vegetation cover (forest and pasture), we will measure soil respiration along with the organic carbon and the microbial biomass of soils during campaigns of wet and dry seasons, with complementary measurements of soil temperature, water and nutrient content. Also, we will reproduce a fire experiment simulating local activity of "slash and burn" to evaluate fire effects. Measurements will be taken after the soil cooled and 1, 3, 5, 7 and 10 days after the fire. Additionally, the carbon stock of the subparcels will be evaluated. Evaluation of the variations of CO2 fluxes and the capacity of adaptation to fire and water content will be done through the comparisons of the different locations, type of soils and concentration of available N as an indicator of nutrient content.

Facilitating nutrient aquisition of black walnut and other hardwoods at plantation establishment

Treesearch

Douglass F. Jacobs; John R. Seifert

2004-01-01

Bareroot hardwood seedlings typically undergo transplant shock immediately following planting before root systems are established. Fertilization at planting may act to minimize transplant shock by reducing nutrient stresses. However, previous work with fertilization of hardwoods at planting has generally relied on fertilizers with nutrient forms immediately available....

Short-term effects of a simulated acid rain upon the growth and nutrient relations of Pinus strobus, L.

Treesearch

Tim Wood; F. H. Bormann

1976-01-01

Acidified precipitation may affect the productivity of forests by altering the availability of plant nutrients of by affecting the ability of trees to absorb and assimilate those nutrients. In this study, the short-term effects of simulated acid rain (pH range 5.6 - 2.3) upon the growth and nutrient relations of Eastern White Pine seedlings (Pinus strobus...

Drug-nutrient interactions.

PubMed

Trovato, A; Nuhlicek, D N; Midtling, J E

1991-11-01

Drug-nutrient interactions are a commonly overlooked aspect of the prescribing practices of physicians. As more pharmaceutical agents become available, attention should be focused on interactions of drugs with foods and nutrients. Although drug-nutrient interactions are not as common as drug-drug interactions, they can have an impact on therapeutic outcome. Drugs can affect nutritional status by altering nutrient absorption, metabolism, utilization or excretion. Food, beverages and mineral or vitamin supplements can affect the absorption and effectiveness of drugs. Knowledge of drug-nutrient interactions can help reduce the incidence of these effects. Physicians should question patients about their dietary habits so that patients can be informed about possible interactions between a prescribed drug and foods and nutrients.

Structure and mechanism of the T-box riboswitches

PubMed Central

Zhang, Jinwei

2015-01-01

In most Gram-positive bacteria, including many clinically devastating pathogens from genera such as Bacillus, Clostridium, Listeria and Staphylococcus, T-box riboswitches sense and regulate intracellular availability of amino acids through a multipartite mRNA-tRNA interaction. The T-box mRNA leaders respond to nutrient starvation by specifically binding cognate tRNAs and sensing whether the bound tRNA is aminoacylated, as a proxy for amino acid availability. Based on this readout, T-boxes direct a transcriptional or translational switch to control the expression of downstream genes involved in various aspects of amino acid metabolism: biosynthesis, transport, aminoacylation, transamidation, etc. Two decades after its discovery, the structural and mechanistic underpinnings of the T-box riboswitch were recently elucidated, producing a wealth of insights into how two structured RNAs can recognize each other with robust affinity and exquisite selectivity. The T-box paradigm exemplifies how natural non-coding RNAs can interact not just through sequence complementarity, but can add molecular specificity by precisely juxtaposing RNA structural motifs, exploiting inherently flexible elements and the biophysical properties of post-transcriptional modifications, ultimately achieving a high degree of shape complementarity through mutually induced fit. The T-box also provides a proof-of-principle that compact RNA domains can recognize minute chemical changes (such as tRNA aminoacylation) on another RNA. The unveiling of the structure and mechanism of the T-box system thus expands our appreciation of the range of capabilities and modes of action of structured non-coding RNAs, and hints at the existence of networks of non-coding RNAs that communicate through both, structural and sequence specificity. PMID:25959893

Limitations to CO2-induced growth enhancement in pot studies.

PubMed

McConnaughay, K D M; Berntson, G M; Bazzaz, F A

1993-07-01

Recently, it has been suggested that small pots may reduce or eliminate plant responses to enriched CO 2 atmospheres due to root restriction. While smaller pot volumes provide less physical space available for root growth, they also provide less nutrients. Reduced nutrient availability alone may reduce growth enhancement under elevated CO 2 . To investigate the relative importance of limited physical rooting space separate from and in conjunction with soil nutrients, we grew plants at ambient and double-ambient CO 2 levels in growth containers of varied volume, shape, nutrient concentration, and total nutrient content. Two species (Abutilon theophrasti, a C 3 dicot with a deep tap root andSetaria faberii, a C 4 monocot with a shallow diffuse root system) were selected for their contrasting physiology and root architecture. Shoot demography was determined weekly and biomass was determined after eight and ten weeks of growth. Increasing total nutrients, either by increasing nutrient concentration or by increasing pot size, increased plant growth. Further, increasing pot size while maintaining equal total nutrients per pot resulted in increased total biomass for both species. CO 2 -induced growth and reproductive yield enhancements were greatest in pots with high nutrient concentrations, regardless of total nutrient content or pot size, and were also mediated by the shape of the pot. CO 2 -induced growth and reproductive yield enhancements were unaffected by pot size (growth) or were greater in small pots (reproductive yield), regardless of total nutrient content, contrary to predictions based on earlier studies. These results suggest that several aspects of growth conditions within pots may influence the CO 2 responses of plants; pot size, pot shape, the concentration and total amount of nutrient additions to pots may lead to over-or underestimates of the CO 2 responses of real-world plants.

Modelling the temporal and spatial distribution of ecological variables in Beibu Gulf

NASA Astrophysics Data System (ADS)

Pan, H.; Huang, L.; Yang, S.; Shi, D.; Pan, W.

2017-12-01

Beibu Gulf is an important semi-enclosed gulf located in northern South China Sea. It is rich in natural resources and its coastal rim is undergoing a rapid economic growth in recent years. Study on the spatial and temporal distribution of ecological variables by the influence of physical and biological processes in Beibu Gulf can provide the theoretical basis for the utilization of resources and environmental protection. Based on the MEC three-dimensional hydrodynamic model, a nutrient-phytoplankton-zooplankton-detritus (NPZD) model was applied to simulate the distribution of ecological variables in Beibu Gulf. The result shows that the ecosystem in Beibu Gulf is significantly influenced by dynamic conditions. In autumn and winter, great amount of nutrient-rich water from western Guangdong coastal area passes through Qiongzhou Strait and flows into Beibu Gulf, with about 108.3×103 t of inorganic nitrogen and 3.7×103 t of phosphate annually, leading to phytoplankton bloom. In summer, most of the nutrients come from rivers so high concentrations of nutrients and chlorophyll-a appear on estuaries. The annual net nutrient inputs from South China Sea into Beibu Gulf are 66.6×103 t for inorganic nitrogen and 4.6×103 t for phosphate. Phytoplankton plays an important role in nutrients' refreshment: a) Absorption by the process of photosynthesis is the biggest nutrient sink. b) Cellular release from dead phytoplankton is the biggest source in inorganic budget, making up for 33.4% of nitrogen consumed by photosynthesis while the process of respiration is the biggest source in phosphate budget, making up for 32.4% of phosphorus consumed by photosynthesis. c) Mineralization from detritus is also a considerable supplement of inorganic nutrients. Overall, biological process has more influence than physical process on the nutrient cycle budget in Beibu Gulf. The comparison of the result with remote sensing and in-situ data indicates that the model is able to simulate the biogeochemical characteristics in Beibu Gulf.

mTORC1 Activator SLC38A9 Is Required to Efflux Essential Amino Acids from Lysosomes and Use Protein as a Nutrient.

PubMed

Wyant, Gregory A; Abu-Remaileh, Monther; Wolfson, Rachel L; Chen, Walter W; Freinkman, Elizaveta; Danai, Laura V; Vander Heiden, Matthew G; Sabatini, David M

2017-10-19

The mTORC1 kinase is a master growth regulator that senses many environmental cues, including amino acids. Activation of mTORC1 by arginine requires SLC38A9, a poorly understood lysosomal membrane protein with homology to amino acid transporters. Here, we validate that SLC38A9 is an arginine sensor for the mTORC1 pathway, and we uncover an unexpectedly central role for SLC38A9 in amino acid homeostasis. SLC38A9 mediates the transport, in an arginine-regulated fashion, of many essential amino acids out of lysosomes, including leucine, which mTORC1 senses through the cytosolic Sestrin proteins. SLC38A9 is necessary for leucine generated via lysosomal proteolysis to exit lysosomes and activate mTORC1. Pancreatic cancer cells, which use macropinocytosed protein as a nutrient source, require SLC38A9 to form tumors. Thus, through SLC38A9, arginine serves as a lysosomal messenger that couples mTORC1 activation to the release from lysosomes of the essential amino acids needed to drive cell growth. Copyright © 2017 Elsevier Inc. All rights reserved.

Ageing induced vascular smooth muscle cell senescence in atherosclerosis.

PubMed

Uryga, Anna K; Bennett, Martin R

2016-04-15

Atherosclerosis is a disease of ageing in that its incidence and prevalence increase with age. However, atherosclerosis is also associated with biological ageing, manifest by a number of typical hallmarks of ageing in the atherosclerotic plaque. Thus, accelerated biological ageing may be superimposed on the effects of chronological ageing in atherosclerosis. Tissue ageing is seen in all cells that comprise the plaque, but particularly in vascular smooth muscle cells (VSMCs). Hallmarks of ageing include evidence of cell senescence, DNA damage (including telomere attrition), mitochondrial dysfunction, a pro-inflammatory secretory phenotype, defects in proteostasis, epigenetic changes, deregulated nutrient sensing, and exhaustion of progenitor cells. In this model, initial damage to DNA (genomic, telomeric, mitochondrial and epigenetic changes) results in a number of cellular responses (cellular senescence, deregulated nutrient sensing and defects in proteostasis). Ultimately, ongoing damage and attempts at repair by continued proliferation overwhelm reparative capacity, causing loss of specialised cell functions, cell death and inflammation. This review summarises the evidence for accelerated biological ageing in atherosclerosis, the functional consequences of cell ageing on cells comprising the plaque, and the causal role that VSMC senescence plays in atherogenesis. © 2015 The Authors. The Journal of Physiology © 2015 The Physiological Society.

The Nutrient-Sensing Hexosamine Biosynthetic Pathway as the Hub of Cancer Metabolic Rewiring.

PubMed

Chiaradonna, Ferdinando; Ricciardiello, Francesca; Palorini, Roberta

2018-06-02

Alterations in glucose and glutamine utilizing pathways and in fatty acid metabolism are currently considered the most significant and prevalent metabolic changes observed in almost all types of tumors. Glucose, glutamine and fatty acids are the substrates for the hexosamine biosynthetic pathway (HBP). This metabolic pathway generates the "sensing molecule" UDP- N -Acetylglucosamine (UDP-Glc N Ac). UDP-Glc N Ac is the substrate for the enzymes involved in protein N - and O -glycosylation, two important post-translational modifications (PTMs) identified in several proteins localized in the extracellular space, on the cell membrane and in the cytoplasm, nucleus and mitochondria. Since protein glycosylation controls several key aspects of cell physiology, aberrant protein glycosylation has been associated with different human diseases, including cancer. Here we review recent evidence indicating the tight association between the HBP flux and cell metabolism, with particular emphasis on the post-transcriptional and transcriptional mechanisms regulated by the HBP that may cause the metabolic rewiring observed in cancer. We describe the implications of both protein O - and N -glycosylation in cancer cell metabolism and bioenergetics; focusing our attention on the effect of these PTMs on nutrient transport and on the transcriptional regulation and function of cancer-specific metabolic pathways.

Extracellular Polymeric Substance Production and Aggregated Bacteria Colonization Influence the Competition of Microbes in Biofilms.

PubMed

Jayathilake, Pahala G; Jana, Saikat; Rushton, Steve; Swailes, David; Bridgens, Ben; Curtis, Tom; Chen, Jinju

2017-01-01

The production of extracellular polymeric substance (EPS) is important for the survival of biofilms. However, EPS production is costly for bacteria and the bacterial strains that produce EPS (EPS+) grow in the same environment as non-producers (EPS-) leading to competition between these strains for nutrients and space. The outcome of this competition is likely to be dependent on factors such as initial attachment, EPS production rate, ambient nutrient levels and quorum sensing. We use an Individual-based Model (IbM) to study the competition between EPS+ and EPS- strains by varying the nature of initial colonizers which can either be in the form of single cells or multicellular aggregates. The microbes with EPS+ characteristics obtain a competitive advantage if they initially colonize the surface as smaller aggregates and are widely spread-out between the cells of EPS-, when both are deposited on the substratum. Furthermore, the results show that quorum sensing-regulated EPS production may significantly reduce the fitness of EPS producers when they initially deposit as aggregates. The results provide insights into how the distribution of bacterial aggregates during initial colonization could be a deciding factor in the competition among different strains in biofilms.

Herbicide glufosinate inhibits yeast growth and extends longevity during wine fermentation.

PubMed

Vallejo, Beatriz; Picazo, Cecilia; Orozco, Helena; Matallana, Emilia; Aranda, Agustín

2017-09-29

Glufosinate ammonium (GA) is a widely used herbicide that inhibits glutamine synthetase. This inhibition leads to internal amino acid starvation which, in turn, causes the activation of different nutrient sensing pathways. GA also inhibits the enzyme of the yeast Saccharomyces cerevisiae in such a way that, although it is not used as a fungicide, it may alter yeast performance in industrial processes like winemaking. We describe herein how GA indeed inhibits the yeast growth of a wine strain during the fermentation of grape juice. In turn, GA extends longevity in a variety of growth media. The biochemical analysis indicates that GA partially inhibits the nutrient sensing TORC1 pathway, which may explain these phenotypes. The GCN2 kinase mutant is hypersensitive to GA. Hence the control of translation and amino acid biosynthesis is required to also deal with the damaging effects of this pesticide. A global metabolomics analysis under winemaking conditions indicated that an increase in amino acid and in polyamines occurred. In conclusion, GA affects many different biochemical processes during winemaking, which provides us with some insights into both the effect of this herbicide on yeast physiology and into the relevance of the metabolic step for connecting nitrogen and carbon metabolism.

Plant root and shoot dynamics during subsurface obstacle interaction

NASA Astrophysics Data System (ADS)

Conn, Nathaniel; Aguilar, Jeffrey; Benfey, Philip; Goldman, Daniel

As roots grow, they must navigate complex underground environments to anchor and retrieve water and nutrients. From gravity sensing at the root tip to pressure sensing along the tip and elongation zone, the complex mechanosensory feedback system of the root allows it to bend towards greater depths and avoid obstacles of high impedance by asymmetrically suppressing cell elongation. Here we investigate the mechanical and physiological responses of roots to rigid obstacles. We grow Maize, Zea mays, plants in quasi-2D glass containers (22cm x 17cm x 1.4cm) filled with photoelastic gel and observe that, regardless of obstacle interaction, smaller roots branch off the primary root when the upward growing shoot (which contains the first leaf) reaches an average length of 40 mm, coinciding with when the first leaf emerges. However, prior to branching, contacts with obstacles result in reduced root growth rates. The growth rate of the root relative to the shoot is sensitive to the angle of the obstacle surface, whereby the relative root growth is greatest for horizontally oriented surfaces. We posit that root growth is prioritized when horizontal obstacles are encountered to ensure anchoring and access to nutrients during later stages of development. NSF Physics of Living Systems.

Potassium fertigation in highbush blueberry increases availability of K and other nutrients in the root zone

USDA-ARS?s Scientific Manuscript database

Fertigation with nitrogen (N) increases growth and production relative to granular N applications in blueberry, but little information is available on whether there is any benefit to fertigating with other nutrients. The plants were grown on raised beds and irrigated using two lines of drip tubing p...

NGEE Arctic Plant Traits: Soil Nutrient Availability, Kougarok Road Mile Marker 64, Seward Peninsula, Alaska, beginning 2016

DOE Office of Scientific and Technical Information (OSTI.GOV)

Verity Salmon; Colleen Iversen; Amy Breen

Soil nutrient availability at all vegetation plots was measured using anion and cation binding resins deployed to vegetation plots at the Kougarok hillslope site located at Kougarok Road Marker 64. Concentrations of ammonia, nitrate, and phosphate in resin extract solutions were determined in the lab.

Natural and anthropogenic drivers of calcium depletion in a northern forest during the last millennium.

PubMed

Leys, Bérangère A; Likens, Gene E; Johnson, Chris E; Craine, Joseph M; Lacroix, Brice; McLauchlan, Kendra K

2016-06-21

The pace and degree of nutrient limitation are among the most critical uncertainties in predicting terrestrial ecosystem responses to global change. In the northeastern United States, forest growth has recently declined along with decreased soil calcium (Ca) availability, suggesting that acid rain has depleted soil Ca to the point where it may be a limiting nutrient. However, it is unknown whether the past 60 y of changes in Ca availability are strictly anthropogenic or partly a natural consequence of long-term ecosystem development. Here, we report a high-resolution millennial-scale record of Ca and 16 other elements from the sediments of Mirror Lake, a 15-ha lake in the White Mountains of New Hampshire surrounded by northern hardwood forest. We found that sedimentary Ca concentrations had been declining steadily for 900 y before regional Euro-American settlement. This Ca decline was not a result of serial episodic disturbances but instead the gradual weathering of soils and soil Ca availability. As Ca availability was declining, nitrogen availability concurrently was increasing. These data indicate that nutrient availability on base-poor, parent materials is sensitive to acidifying processes on millennial timescales. Forest harvesting and acid rain in the postsettlement period mobilized significant amounts of Ca from watershed soils, but these effects were exacerbated by the long-term pattern. Shifting nutrient limitation can potentially occur within 10,000 y of ecosystem development, which alters our assessments of the speed and trajectory of nutrient limitation in forests, and could require reformulation of global models of forest productivity.

Natural and anthropogenic drivers of calcium depletion in a northern forest during the last millennium

PubMed Central

Leys, Bérangère A.; Likens, Gene E.; Craine, Joseph M.; Lacroix, Brice; McLauchlan, Kendra K.

2016-01-01

The pace and degree of nutrient limitation are among the most critical uncertainties in predicting terrestrial ecosystem responses to global change. In the northeastern United States, forest growth has recently declined along with decreased soil calcium (Ca) availability, suggesting that acid rain has depleted soil Ca to the point where it may be a limiting nutrient. However, it is unknown whether the past 60 y of changes in Ca availability are strictly anthropogenic or partly a natural consequence of long-term ecosystem development. Here, we report a high-resolution millennial-scale record of Ca and 16 other elements from the sediments of Mirror Lake, a 15-ha lake in the White Mountains of New Hampshire surrounded by northern hardwood forest. We found that sedimentary Ca concentrations had been declining steadily for 900 y before regional Euro-American settlement. This Ca decline was not a result of serial episodic disturbances but instead the gradual weathering of soils and soil Ca availability. As Ca availability was declining, nitrogen availability concurrently was increasing. These data indicate that nutrient availability on base-poor, parent materials is sensitive to acidifying processes on millennial timescales. Forest harvesting and acid rain in the postsettlement period mobilized significant amounts of Ca from watershed soils, but these effects were exacerbated by the long-term pattern. Shifting nutrient limitation can potentially occur within 10,000 y of ecosystem development, which alters our assessments of the speed and trajectory of nutrient limitation in forests, and could require reformulation of global models of forest productivity. PMID:27298361

A forestry GIS-based study on evaluating the potential of imaging spectroscopy in mapping forest land fertility

NASA Astrophysics Data System (ADS)

Mõttus, Matti; Takala, Tuure

2014-12-01

Fertility, or the availability of nutrients and water, controls forest productivity. It affects its carbon sequestration, and thus the forest's effect on climate, as well as its commercial value. Although the availability of nutrients cannot be measured directly using remote sensing methods, fertility alters several vegetation traits detectable from the reflectance spectra of the forest stand, including its pigment content and water stress. However, forest reflectance is also influenced by other factors, such as species composition and stand age. Here, we present a case study demonstrating how data obtained using imaging spectroscopy is correlated with site fertility. The study was carried out in Hyytiälä, Finland, in the southern boreal forest zone. We used a database of state-owned forest stands including basic forestry variables and a site fertility index. To test the suitability of imaging spectroscopy with different spatial and spectral resolutions for site fertility mapping, we performed two airborne acquisitions using different sensor configurations. First, the sensor was flown at a high altitude with high spectral resolution resulting in a pixel size in the order of a tree crown. Next, the same area was flown to provide reflectance data with sub-meter spatial resolution. However, to maintain usable signal-to-noise ratios, several spectral channels inside the sensor were combined, thus reducing spectral resolution. We correlated a number of narrowband vegetation indices (describing canopy biochemical composition, structure, and photosynthetic activity) on site fertility. Overall, site fertility had a significant influence on the vegetation indices but the strength of the correlation depended on dominant species. We found that high spatial resolution data calculated from the spectra of sunlit parts of tree crowns had the strongest correlation with site fertility.

The loss of ecosystem services due to land degradation. Integration of mechanistic and probabilistic models in an Ethiopian case study

NASA Astrophysics Data System (ADS)

Cerretelli, Stefania; Poggio, Laura; Gimona, Alessandro; Peressotti, Alessandro; Black, Helaina

2017-04-01

Land and soil degradation are widespread especially in dry and developing countries such as Ethiopia. Land degradation leads to ecosystems services (ESS) degradation, because it causes the depletion and loss of several soil functions. Ethiopia's farmland faces intense degradation due to deforestation, agricultural land expansion, land overexploitation and overgrazing. In this study we modelled the impact of physical factors on ESS degradation, in particular soil erodibility, carbon storage and nutrient retention, in the Ethiopian Great Rift Valley, northwestern of Hawassa. We used models of the Sediment retention/loss, the Nutrient Retention/loss (from the software suite InVEST) and Carbon Storage. To run the models we coupled soil local data (such as soil organic carbon, soil texture) with remote sensing data as input in the parametrization phase, e.g. to derive a land use map, to calculate the aboveground and belowground carbon, the evapotraspiration coefficient and the capacity of vegetation to retain nutrient. We then used spatialised Bayesian Belief Networks (sBBNs) predicting ecosystem services degradation on the basis of the results of the three mechanistic models. The results show i) the importance of mapping of ESS degradation taking into consideration the spatial heterogeneity and the cross-correlations between impacts ii) the fundamental role of remote sensing data in monitoring and modelling in remote, data-poor areas and iii) the important role of spatial BBNs in providing spatially explicit measures of risk and uncertainty. This approach could help decision makers to identify priority areas for intervention in order to reduce land and ecosystem services degradation.

"Land-Cover Conversion in Amazonia, The Role of ENV" Ironment and Substrate composition in Modifying SOI

NASA Technical Reports Server (NTRS)

Roberts, Dar A.; Chadwick, Oliver A.; Batista, Getulio T.

2003-01-01

LBA research from the first phase of LBA focused on three broad categories: 1) mapping land cover and quantifying rates of change, persistence of pasture, and area of recovering forest; 2) evaluating the role of environmental factors and land-use history on soil biogeochemistry; and 3) quantifying the natural and human controls on stream nutrient concentrations. The focus of the research was regional, concentrating primarily in the state of RondBnia, but also included land-cover mapping in the vicinity of Maraba, Para, and Manaus, Amazonas. Remote sensing analysis utilized Landsat Thematic Mapper (TM) and Multispectral Scanner (MS S) data to map historical patterns of land-cover change. Specific questions addressed by the remote sensing component of the research included: 1) what is the areal extent of dominant land-cover classes? 2) what are the rates of change of dominant land cover through processes of deforestation, disturbance and regeneration? and 3) what are the dynamic properties of each class that characterize temporal variability, duration, and frequency of repeat disturbance? Biogeochemical analysis focused on natural variability and impacts of land-use/land-cover changes on soil and stream biogeochemical properties at the regional scale. An emphasis was given to specific soil properties considered to be primary limiting factors regionally, including phosphorus, nitrogen, base cations and cation-exchange properties. Stream sampling emphasized the relative effects of the rates and timing of land-cover change on stream nutrients, demonstrating that vegetation conversion alone does not impact nutrients as much as subsequent land use and urbanization.

A fructose receptor functions as a nutrient sensor in the Drosophila brain

PubMed Central

Miyamoto, Tetsuya; Slone, Jesse; Song, Xiangyu; Amrein, Hubert

2012-01-01

SUMMARY Internal nutrient sensors play important roles in feeding behavior, yet their molecular structure and mechanism of action are poorly understood. Using Ca2+ imaging and behavioral assays, we show that the Gustatory Receptor 43a functions as a narrowly tuned fructose receptor in taste neurons. Remarkably, GR43a also functions as a fructose receptor in the brain. Interestingly, hemolymph fructose levels are tightly linked to feeding status: after nutritious carbohydrate consumption, fructose levels rise several fold and reach a concentration sufficient to activate GR43a in the brain. By using different feeding paradigms and artificial activation of Gr43a-expressing brain neurons, we show that GR43a is both necessary and sufficient to sense hemolymph fructose and promote feeding in hungry flies, but suppress feeding in satiated flies. Thus, our studies indicate that the Gr43a-expressing brain neurons function as a nutrient sensor for hemolymph fructose and assign opposing valence to feeding experiences in a satiation-dependent manner. PMID:23178127

Construction of web-based nutrition education contents and searching engine for usage of healthy menu of children

PubMed Central

Lee, Tae-Kyong; Chung, Hea-Jung; Park, Hye-Kyung; Lee, Eun-Ju; Nam, Hye-Seon; Jung, Soon-Im; Cho, Jee-Ye; Lee, Jin-Hee; Kim, Gon; Kim, Min-Chan

2008-01-01

A diet habit, which is developed in childhood, lasts for a life time. In this sense, nutrition education and early exposure to healthy menus in childhood is important. Children these days have easy access to the internet. Thus, a web-based nutrition education program for children is an effective tool for nutrition education of children. This site provides the material of the nutrition education for children with characters which are personified nutrients. The 151 menus are stored in the site together with video script of the cooking process. The menus are classified by the criteria based on age, menu type and the ethnic origin of the menu. The site provides a search function. There are three kinds of search conditions which are key words, menu type and "between" expression of nutrients such as calorie and other nutrients. The site is developed with the operating system Windows 2003 Server, the web server ZEUS 5, development language JSP, and database management system Oracle 10 g. PMID:20126375

Food Web Architecture and Basal Resources Interact to Determine Biomass and Stoichiometric Cascades along a Benthic Food Web

PubMed Central

Guariento, Rafael D.; Carneiro, Luciana S.; Caliman, Adriano; Leal, João J. F.; Bozelli, Reinaldo L.; Esteves, Francisco A.

2011-01-01

Understanding the effects of predators and resources on primary producers has been a major focus of interest in ecology. Within this context, the trophic cascade concept especially concerning the pelagic zone of lakes has been the focus of the majority of these studies. However, littoral food webs could be especially interesting because base trophic levels may be strongly regulated by consumers and prone to be light limited. In this study, the availability of nutrients and light and the presence of an omnivorous fish (Hyphessobrycon bifasciatus) were manipulated in enclosures placed in a humic coastal lagoon (Cabiúnas Lagoon, Macaé – RJ) to evaluate the individual and interactive effects of resource availability (nutrients and light) and food web configuration on the biomass and stoichiometry of periphyton and benthic grazers. Our findings suggest that light and nutrients interact to determine periphyton biomass and stoichiometry, which propagates to the consumer level. We observed a positive effect of the availability of nutrients on periphytic biomass and grazers' biomass, as well as a reduction of periphytic C∶N∶P ratios and an increase of grazers' N and P content. Low light availability constrained the propagation of nutrient effects on periphyton biomass and induced higher periphytic C∶N∶P ratios. The effects of fish presence strongly interacted with resource availability. In general, a positive effect of fish presence was observed for the total biomass of periphyton and grazer's biomass, especially with high resource availability, but the opposite was found for periphytic autotrophic biomass. Fish also had a significant effect on periphyton stoichiometry, but no effect was observed on grazers' stoichiometric ratios. In summary, we observed that the indirect effect of fish predation on periphyton biomass might be dependent on multiple resources and periphyton nutrient stoichiometric variation can affect consumers' stoichiometry. PMID:21789234

The Consequences of Precipitation Seasonality for Mediterranean-Ecosystem Vegetation of South Africa.

PubMed

Cramer, Michael D; Hoffman, M Timm

2015-01-01

Globally, mediterranean-climate ecosystem vegetation has converged on an evergreen, sclerophyllous and shrubby growth form. The particular aspects of mediterranean-climate regions that contribute to this convergence include summer droughts and relatively nutrient-poor soils. We hypothesised that winter-precipitation implies stressful summer droughts and leaches soils due to greater water availability (i.e. balance between precipitation and potential evapotranspiration; P-PET) during cold periods. We conducted a comparative analysis of normalised difference vegetation indices (NDVI) and edaphic and climate properties across the biomes of South Africa. NDVI was strongly correlated with both precipitation and P-PET (r2 = 0.8). There was no evidence, however, that winter-precipitation reduces NDVI in comparison to similar amounts of summer-precipitation. Base saturation (BS), a measure of soil leaching was, however, negatively related to P-PET (r2 = 0.64). This led to an interaction between P-PET and BS in determining NDVI, indicating the existence of a trade-off between water availability and soil nutrients that enables NDVI to increase with precipitation, despite negative consequences for soil nutrient availability. The mechanism of this trade-off is suggested to be that water increases nutrient accessibility. This implies that along with nutrient-depauperate geologies and long periods of time since glaciation, the winter-precipitation may have contributed to the highly leached status of the soils. Since many of the ecophysiological characteristics of mediterranean-ecosystem flora are associated with low nutrient availabilities (e.g. evergreen foliage, sclerophylly, cluster roots), we conclude that mediterranean-climates promote convergence of growth-forms in these regions through high leaching capacity.

The Consequences of Precipitation Seasonality for Mediterranean-Ecosystem Vegetation of South Africa

PubMed Central

2015-01-01

Globally, mediterranean-climate ecosystem vegetation has converged on an evergreen, sclerophyllous and shrubby growth form. The particular aspects of mediterranean-climate regions that contribute to this convergence include summer droughts and relatively nutrient-poor soils. We hypothesised that winter-precipitation implies stressful summer droughts and leaches soils due to greater water availability (i.e. balance between precipitation and potential evapotranspiration; P–PET) during cold periods. We conducted a comparative analysis of normalised difference vegetation indices (NDVI) and edaphic and climate properties across the biomes of South Africa. NDVI was strongly correlated with both precipitation and P–PET (r2 = 0.8). There was no evidence, however, that winter-precipitation reduces NDVI in comparison to similar amounts of summer-precipitation. Base saturation (BS), a measure of soil leaching was, however, negatively related to P–PET (r2 = 0.64). This led to an interaction between P–PET and BS in determining NDVI, indicating the existence of a trade-off between water availability and soil nutrients that enables NDVI to increase with precipitation, despite negative consequences for soil nutrient availability. The mechanism of this trade-off is suggested to be that water increases nutrient accessibility. This implies that along with nutrient-depauperate geologies and long periods of time since glaciation, the winter-precipitation may have contributed to the highly leached status of the soils. Since many of the ecophysiological characteristics of mediterranean-ecosystem flora are associated with low nutrient availabilities (e.g. evergreen foliage, sclerophylly, cluster roots), we conclude that mediterranean-climates promote convergence of growth-forms in these regions through high leaching capacity. PMID:26650081

Fatal attraction: vegetation responses to nutrient inputs attract herbivores to infectious anthrax carcass sites.

PubMed

Turner, Wendy C; Kausrud, Kyrre L; Krishnappa, Yathin S; Cromsigt, Joris P G M; Ganz, Holly H; Mapaure, Isaac; Cloete, Claudine C; Havarua, Zepee; Küsters, Martina; Getz, Wayne M; Stenseth, Nils Chr

2014-11-22

Parasites can shape the foraging behaviour of their hosts through cues indicating risk of infection. When cues for risk co-occur with desired traits such as forage quality, individuals face a trade-off between nutrient acquisition and parasite exposure. We evaluated how this trade-off may influence disease transmission in a 3-year experimental study of anthrax in a guild of mammalian herbivores in Etosha National Park, Namibia. At plains zebra (Equus quagga) carcass sites we assessed (i) carcass nutrient effects on soils and grasses, (ii) concentrations of Bacillus anthracis (BA) on grasses and in soils, and (iii) herbivore grazing behaviour, compared with control sites, using motion-sensing camera traps. We found that carcass-mediated nutrient pulses improved soil and vegetation, and that BA is found on grasses up to 2 years after death. Host foraging responses to carcass sites shifted from avoidance to attraction, and ultimately to no preference, with the strength and duration of these behavioural responses varying among herbivore species. Our results demonstrate that animal carcasses alter the environment and attract grazing hosts to parasite aggregations. This attraction may enhance transmission rates, suggesting that hosts are limited in their ability to trade off nutrient intake with parasite avoidance when relying on indirect cues. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

Taste-nutrient relationships in commonly consumed foods.

PubMed

van Dongen, Mirre Viskaal; van den Berg, Marjolijn C; Vink, Nicole; Kok, Frans J; de Graaf, Cees

2012-07-14

Taste is expected to represent a food's nutrient content. The objective was to investigate whether taste acts as nutrient-sensor, within the context of the current diet, which is high in processed foods. Intensities of the five basic tastes of fifty commonly consumed foods were rated by nineteen subjects (aged 21·0 (SD 1·7) years, BMI 21·5 (SD 2·0) kg/m(2)). Linear regression was used to test associations between taste and nutrient contents. Food groups based on taste were identified using cluster analysis; nutrient content was compared between food groups, using ANOVA. Sweetness was associated with mono- and disaccharides (R(2) 0·45, P < 0·01). Saltiness and savouriness were correlated, with r 0·92 (P < 0·01) and both were associated with Na (both: R(2) 0·33, P < 0·01) and protein (R(2) 0·27, P < 0·01 and R(2) 0·33, P < 0·01, respectively). Cluster analysis indicated four food groups: neutral, salty and savoury, sweet-sour and sweet foods. Mono- and disaccharide content was highest in sweet foods (P < 0·01). In salty and savoury foods, protein content (P = 0·01 with sweet-sour foods, not significant with neutral or sweet foods) and Na content (P < 0·05) were the highest. Associations were more pronounced in raw and moderately processed foods, than in highly processed foods. The findings suggest that sweetness, saltiness and savouriness signal nutrient content, particularly for simple sugars, protein and Na. In highly processed foods, however, the ability to sense nutrient content based on taste seems limited.

Detection of wine grape nutrient levels using visible and near infrared 1nm spectral resolution remote sensing

NASA Astrophysics Data System (ADS)

Anderson, Grant; van Aardt, Jan; Bajorski, Peter; Vanden Heuvel, Justine

2016-05-01

The grape industry relies on regular crop assessment to aid in the day-to-day and seasonal management of their crop. More specifically, there are six key nutrients of interest to viticulturists in the growing of wine grapes, namely nitrogen, potassium, phosphorous, magnesium, zinc and boron. Traditional methods of determining the levels of these nutrients are through collection and chemical analysis of petiole samples from the grape vines themselves. We collected ground-level observations of the spectra of the grape vines, using a hyperspectral spectrometer (0.4-2.5um), at the same time that petioles samples were harvested. We then interpolated the data into a consistent 1 nm spectral resolution before comparing it to the nutrient data collected. This nutrient data came from both the industry standard petiole analysis, as well as an additional leaf-level analysis. The data were collected for two different grape cultivars, both during bloom and veraison periods to provide variability, while also considering the impact of temporal/seasonal change. A narrow-band NDI (Normalized Difference Index) approach, as well as a simple ratio index, was used to determine the correlation of the reflectance data to the nutrient data. This analysis was limited to the silicon photodiode range to increase the utility of our approach for wavelength-specific cameras (via spectral filters) in a low cost drone platform. The NDI generated correlation coefficients were as high as 0.80 and 0.88 for bloom and veraison, respectively. The ratio index produced correlation coefficient results that are the same at two decimal places with 0.80 and 0.88. These results bode well for eventual non-destructive, accurate and precise assessment of vineyard nutrient status.

Compost supplementation with nutrients and microorganisms in composting process.

PubMed

Sánchez, Óscar J; Ospina, Diego A; Montoya, Sandra

2017-11-01

The composting is an aerobic, microorganism-mediated, solid-state fermentation process by which different organic materials are transformed into more stable compounds. The product obtained is the compost, which contributes to the improvement of physical, chemical and microbiological properties of the soil. However, the compost usage in agriculture is constrained because of its long-time action and reduced supply of nutrients to the crops. To enhance the content of nutrients assimilable by the plants in the compost, its supplementation with nutrients and inoculation with microorganisms have been proposed. The objective of this work was to review the state of the art on compost supplementation with nutrients and the role played by the microorganisms involved (or added) in their transformation during the composting process. The phases of composting are briefly compiled and different strategies for supplementation are analyzed. The utilization of nitrogenous materials and addition of microorganisms fixing nitrogen from the atmosphere or oxidizing ammonia into more assimilable for plants nitrogenous forms are analyzed. Several strategies for nitrogen conservation during composting are presented as well. The supplementation with phosphorus and utilization of microorganisms solubilizing phosphorus and potassium are also discussed. Main groups of microorganisms relevant during the composting process are described as well as most important strategies to identify them. In general, the development of this type of nutrient-enriched bio-inputs requires research and development not only in the supplementation of compost itself, but also in the isolation and identification of microorganisms and genes allowing the degradation and conversion of nitrogenous substances and materials containing potassium and phosphorus present in the feedstocks undergoing the composting process. In this sense, most important research trends and strategies to increase nutrient content in the compost are provided in this work. Copyright © 2017 Elsevier Ltd. All rights reserved.

Database of nutrient digestibility’s of traditional and novel feed ingredients for trout and hybrid striped bass

USDA-ARS?s Scientific Manuscript database

The determination of nutrient digestibility’s in specific ingredients and diets for fish has been an area of active research for decades. The Apparent Digestibility Coefficients (ADC), the percentage of nutrients in an ingredient that are available to the fish, is information needed by researchers,...

Wastewater and Sludge Nutrient Utilization in Forest Ecosystems

Treesearch

D.G. Brockway; D.H. Urie; P.V. Nguyen; J.B. Hart

1986-01-01

Although forest ecosystems have evolved efficient mechanisms to assimilate and retain modest levels of annual geochemical input, their productivity is frequently limited by low levels of available nutrients. A review of research studies conducted in the major U.S. forest regions indicates that the nutrients and organic matter in wastewater and sludge representa...

Nutrient Supplying Potential of Different Spent Mushroom Substrate Preparations as Soil Amendment in a Potting Media

NASA Astrophysics Data System (ADS)

Ultra, VU, Jr.; Ong Sotto, JME; Punzalan, MR

2018-03-01

A three consecutive cropping experiment was conducted to evaluate the nutrient supplying potential of different preparations of the spent mushroom substrate as an amendment of growing media for potted plants using pechay as test plant. There are 12 treatment combinations consisted 4 types of growing media containing soil alone and mixtures of soil with fresh SMS (FSMS), weathered SMS (WSMS) and carbonized SMS (CSMS) in combination with 0%, 50% or 100% recommended rate (RR) of nitrogen fertilizer. Succeeding two trials were conducted on the same pots and treatment assignments. The high yield of pechay during the first and second crop was observed on WSMS and CSMS treatments FSMS media produced high yields only during the 3rd crop. Yield was increased by N fertilizer in WSMS and CSMS treatments but not in FSMS. The growth differences is attributed to differences in available nutrients and C/N ratio between treatments. WSMS and CSMS increased the available N while FSMS immobilized N and other nutrients indicting that weathered SMS and carbonized SMS are more suitable as a component of potting media or as soil amendments without detrimental effect on immobilization and availability of nutrients.

Metabolic traits of pathogenic streptococci.

PubMed

Willenborg, Jörg; Goethe, Ralph

2016-11-01

Invasive and noninvasive diseases caused by facultative pathogenic streptococci depend on their equipment with virulence factors and on their ability to sense and adapt to changing nutrients in different host environments. The knowledge of the principal metabolic mechanisms which allow these bacteria to recognize and utilize nutrients in host habitats is a prerequisite for our understanding of streptococcal pathogenicity and the development of novel control strategies. This review aims to summarize and compare the central carbohydrate metabolic and amino acid biosynthetic pathways of a selected group of streptococcal species, all belonging to the naso-oropharyngeal microbiome in humans and/or animals. We also discuss the urgent need of comprehensive metabolomics approaches for a better understanding of the streptococcal metabolism during host-pathogen interaction. © 2016 Federation of European Biochemical Societies.

The biogeochemical heterogeneity of tropical forests.

PubMed

Townsend, Alan R; Asner, Gregory P; Cleveland, Cory C

2008-08-01

Tropical forests are renowned for their biological diversity, but also harbor variable combinations of soil age, chemistry and susceptibility to erosion or tectonic uplift. Here we contend that the combined effects of this biotic and abiotic diversity promote exceptional biogeochemical heterogeneity at multiple scales. At local levels, high plant diversity creates variation in chemical and structural traits that affect plant production, decomposition and nutrient cycling. At regional levels, myriad combinations of soil age, soil chemistry and landscape dynamics create variation and uncertainty in limiting nutrients that do not exist at higher latitudes. The effects of such heterogeneity are not well captured in large-scale estimates of tropical ecosystem function, but we suggest new developments in remote sensing can help bridge the gap.

Refuse dumps from leaf-cutting ant nests reduce the intensity of above-ground competition among neighboring plants in a Patagonian steppe

NASA Astrophysics Data System (ADS)

Farji-Brener, Alejandro G.; Lescano, María Natalia

2017-11-01

In arid environments, the high availability of sunlight due to the scarcity of trees suggests that plant competition take place mainly belowground for water and nutrients. However, the occurrence of soil disturbances that increase nutrient availability and thereby promote plant growth may enhance shoot competition between neighboring plants. We conducted a greenhouse experiment to evaluate the influence of the enriched soil patches generated by the leaf-cutting ant, Acromyrmex lobicornis, on the performance of the alien forb Carduus thoermeri (Asteraceae) under different intraspecific competition scenarios. Our results showed that substrate type and competition scenario affected mainly aboveground plant growth. As expected, plants growing without neighbors and in nutrient-rich ant refuse dumps showed more aboveground biomass than plants growing with neighbors and in nutrient-poor steppe soils. However, aboveground competition was more intense in nutrient-poor substrates: plants under shoot and full competition growing in the nutrient-rich ant refuse dumps showed higher biomass than those growing on steppe soils. Belowground biomass was similar among focal plants growing under different substrate type. Our results support the traditional view that increments in resource availability reduce competition intensity. Moreover, the fact that seedlings in this sunny habitat mainly compete aboveground illustrates how limiting factors may be scale-dependent and change in importance as plants grow.

Dynamics of Inorganic Nutrients in Intertidal Sediments: Porewater, Exchangeable, and Intracellular Pools

PubMed Central

Garcia-Robledo, Emilio; Bohorquez, Julio; Corzo, Alfonso; Jimenez-Arias, Juan L.; Papaspyrou, Sokratis

2016-01-01

The study of inorganic nutrients dynamics in shallow sediments usually focuses on two main pools: porewater (PW) nutrients and exchangeable (EX) ammonium and phosphate. Recently, it has been found that microphytobenthos (MPB) and other microorganisms can accumulate large amounts of nutrients intracellularly (IC), highlighting the biogeochemical importance of this nutrient pool. Storing nutrients could support the growth of autotrophs when nutrients are not available, and could also provide alternative electron acceptors for dissimilatory processes such as nitrate reduction. Here, we studied the magnitude and relative importance of these three nutrient pools (PW, IC, and EX) and their relation to chlorophylls (used as a proxy for MPB abundance) and organic matter (OM) contents in an intertidal mudflat of Cadiz Bay (Spain). MPB was localized in the first 4 mm of the sediment and showed a clear seasonal pattern; highest chlorophylls content was found during autumn and lowest during spring-summer. The temporal and spatial distribution of nutrients pools and MPB were largely correlated. Ammonium was higher in the IC and EX fractions, representing on average 59 and 37% of the total ammonium pool, respectively. Similarly, phosphate in the IC and EX fractions accounted on average for 40 and 31% of the total phosphate pool, respectively. Nitrate in the PW was low, suggesting low nitrification activity and rapid consumption. Nitrate accumulated in the IC pool during periods of moderate MPB abundance, being up to 66% of the total nitrate pool, whereas it decreased when chlorophyll concentration peaked likely due to a high nitrogen demand. EX-Nitrate accounted for the largest fraction of total sediment nitrate, 66% on average. The distribution of EX-Nitrate was significantly correlated with chlorophyll and OM, which probably indicates a relation of this pool to an increased availability of sites for ionic adsorption. This EX-Nitrate pool could represent an alternative nitrate source with significant concentrations available to the microbial community, deeper in the sediment below the oxic layer. PMID:27303370

Nutrient availability affects floral scent much less than other floral and vegetative traits in Lithophragma bolanderi.

PubMed

Friberg, Magne; Waters, Mia T; Thompson, John N

2017-09-01

Many plant-pollinator interactions are mediated by floral scents that can vary among species, among populations within species and even among individuals within populations. This variation could be innate and unaffected by the environment, but, because many floral volatiles have amino-acid precursors, scent variation also could be affected by differences in nutrient availability among environments. In plants that have coevolved with specific pollinators, natural selection is likely to favour low phenotypic plasticity in floral scent even under different conditions of nutrient availability if particular scents or scent combinations are important for attracting local pollinators. Clonal pairs of multiple seed-families of two Lithophragma bolanderi (Saxifragaceae) populations were subjected to a high and a low nutrient treatment. These plants are pollinated primarily by host-specific Greya moths. It was evaluated how nutrient treatment affected variation in floral scent relative to other vegetative and reproductive traits. Floral scent strength (the per-flower emission rate) and composition were unaffected by nutrient treatment, but low-nutrient plants produced fewer and lighter leaves, fewer scapes and fewer flowers than high-nutrient plants. The results held in both populations, which differed greatly in the number and composition of floral scents produced. The results reveal a strong genetic component both to scent composition and emission level, and partly contrasts with the only previous study that has assessed the susceptibility of floral volatile signals to variation in the abundance of nutrients. These results, and the tight coevolutionary relationship between Lithophragma plants and their specialized Greya moth pollinators, indicate that reproductive traits important to coevolving interactions, such as the floral scent of L. bolanderi, may be locally specialized and more canalized than other traits important for plant fitness. © The Author 2017. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com

Dynamics of Inorganic Nutrients in Intertidal Sediments: Porewater, Exchangeable, and Intracellular Pools.

PubMed

Garcia-Robledo, Emilio; Bohorquez, Julio; Corzo, Alfonso; Jimenez-Arias, Juan L; Papaspyrou, Sokratis

2016-01-01

The study of inorganic nutrients dynamics in shallow sediments usually focuses on two main pools: porewater (PW) nutrients and exchangeable (EX) ammonium and phosphate. Recently, it has been found that microphytobenthos (MPB) and other microorganisms can accumulate large amounts of nutrients intracellularly (IC), highlighting the biogeochemical importance of this nutrient pool. Storing nutrients could support the growth of autotrophs when nutrients are not available, and could also provide alternative electron acceptors for dissimilatory processes such as nitrate reduction. Here, we studied the magnitude and relative importance of these three nutrient pools (PW, IC, and EX) and their relation to chlorophylls (used as a proxy for MPB abundance) and organic matter (OM) contents in an intertidal mudflat of Cadiz Bay (Spain). MPB was localized in the first 4 mm of the sediment and showed a clear seasonal pattern; highest chlorophylls content was found during autumn and lowest during spring-summer. The temporal and spatial distribution of nutrients pools and MPB were largely correlated. Ammonium was higher in the IC and EX fractions, representing on average 59 and 37% of the total ammonium pool, respectively. Similarly, phosphate in the IC and EX fractions accounted on average for 40 and 31% of the total phosphate pool, respectively. Nitrate in the PW was low, suggesting low nitrification activity and rapid consumption. Nitrate accumulated in the IC pool during periods of moderate MPB abundance, being up to 66% of the total nitrate pool, whereas it decreased when chlorophyll concentration peaked likely due to a high nitrogen demand. EX-Nitrate accounted for the largest fraction of total sediment nitrate, 66% on average. The distribution of EX-Nitrate was significantly correlated with chlorophyll and OM, which probably indicates a relation of this pool to an increased availability of sites for ionic adsorption. This EX-Nitrate pool could represent an alternative nitrate source with significant concentrations available to the microbial community, deeper in the sediment below the oxic layer.

Biogeographic differences in soil biota promote invasive grass response to nutrient addition relative to co-occurring species despite lack of belowground enemy release.

PubMed

Broadbent, Arthur A D; Stevens, Carly J; Ostle, Nicholas J; Orwin, Kate H

2018-03-01

Multiple plant species invasions and increases in nutrient availability are pervasive drivers of global environmental change that often co-occur. Many plant invasion studies, however, focus on single-species or single-mechanism invasions, risking an oversimplification of a multifaceted process. Here, we test how biogeographic differences in soil biota, such as belowground enemy release, interact with increases in nutrient availability to influence invasive plant growth. We conducted a greenhouse experiment using three co-occurring invasive grasses and one native grass. We grew species in live and sterilized soil from the invader's native (United Kingdom) and introduced (New Zealand) ranges with a nutrient addition treatment. We found no evidence for belowground enemy release. However, species' responses to nutrients varied, and this depended on soil origin and sterilization. In live soil from the introduced range, the invasive species Lolium perenne L. responded more positively to nutrient addition than co-occurring invasive and native species. In contrast, in live soil from the native range and in sterilized soils, there were no differences in species' responses to nutrients. This suggests that the presence of soil biota from the introduced range allowed L. perenne to capture additional nutrients better than co-occurring species. Considering the globally widespread nature of anthropogenic nutrient additions to ecosystems, this effect could be contributing to a global homogenization of flora and the associated losses in native species diversity.

Assessing the impact of nutrient enrichment in estuaries: susceptibility to eutrophication.

PubMed

Painting, S J; Devlin, M J; Malcolm, S J; Parker, E R; Mills, D K; Mills, C; Tett, P; Wither, A; Burt, J; Jones, R; Winpenny, K

2007-01-01

The main aim of this study was to develop a generic tool for assessing risks and impacts of nutrient enrichment in estuaries. A simple model was developed to predict the magnitude of primary production by phytoplankton in different estuaries from nutrient input (total available nitrogen and/or phosphorus) and to determine likely trophic status. In the model, primary production is strongly influenced by water residence times and relative light regimes. The model indicates that estuaries with low and moderate light levels are the least likely to show a biological response to nutrient inputs. Estuaries with a good light regime are likely to be sensitive to nutrient enrichment, and to show similar responses, mediated only by site-specific geomorphological features. Nixon's scale was used to describe the relative trophic status of estuaries, and to set nutrient and chlorophyll thresholds for assessing trophic status. Estuaries identified as being eutrophic may not show any signs of eutrophication. Additional attributes need to be considered to assess negative impacts. Here, likely detriment to the oxygen regime was considered, but is most applicable to areas of restricted exchange. Factors which limit phytoplankton growth under high nutrient conditions (water residence times and/or light availability) may favour the growth of other primary producers, such as macrophytes, which may have a negative impact on other biological communities. The assessment tool was developed for estuaries in England and Wales, based on a simple 3-category typology determined by geomorphology and relative light levels. Nixon's scale needs to be validated for estuaries in England and Wales, once more data are available on light levels and primary production.

Mathematical modelling of the influenced of diffusion rate on macro nutrient availability in paddy field

NASA Astrophysics Data System (ADS)

Renny; Supriyanto

2018-04-01

Nutrition is the chemical compounds that needed by the organism for the growth process. In plants, nutrients are organic or inorganic compounds that are absorbed from the roots of the soil. It consist of macro and micro nutrient. Macro nutrients are nutrition that needed by plants in large quantities, such as, nitrogen, calcium, pottacium, magnesium, and sulfur. The total soil nutrient is the difference between the input nutrient and the output nutrients. Input nutrients are nutrient that derived from the decomposition of organic substances. Meanwhile, the output nutrient consists of the nutrients that absorbed by plant roots (uptake), the evaporated nutrients (volatilized) and leached nutrients. The nutrient transport can be done through diffusion process. The diffusion process is essential in removing the nutrient from one place to the root surface. It will cause the rate of absorption of nutrient by the roots will be greater. Nutrient concept in paddy filed can be represented into a mathematical modelling, by making compartment models. The rate of concentration change in the compartment model forms a system of homogeneous linear differential equations. In this research, we will use Laplaces transformation to solve the compartment model and determined the dynamics of macro nutrition due to diffusion process.

Short-term effect of nutrient availability and rainfall distribution on biomass production and leaf nutrient content of savanna tree species.

PubMed

Barbosa, Eduardo R M; Tomlinson, Kyle W; Carvalheiro, Luísa G; Kirkman, Kevin; de Bie, Steven; Prins, Herbert H T; van Langevelde, Frank

2014-01-01

Changes in land use may lead to increased soil nutrient levels in many ecosystems (e.g. due to intensification of agricultural fertilizer use). Plant species differ widely in their response to differences in soil nutrients, and for savannas it is uncertain how this nutrient enrichment will affect plant community dynamics. We set up a large controlled short-term experiment in a semi-arid savanna to test how water supply (even water supply vs. natural rainfall) and nutrient availability (no fertilisation vs. fertilisation) affects seedlings' above-ground biomass production and leaf-nutrient concentrations (N, P and K) of broad-leafed and fine-leafed tree species. Contrary to expectations, neither changes in water supply nor changes in soil nutrient level affected biomass production of the studied species. By contrast, leaf-nutrient concentration did change significantly. Under regular water supply, soil nutrient addition increased the leaf phosphorus concentration of both fine-leafed and broad-leafed species. However, under uneven water supply, leaf nitrogen and phosphorus concentration declined with soil nutrient supply, this effect being more accentuated in broad-leafed species. Leaf potassium concentration of broad-leafed species was lower when growing under constant water supply, especially when no NPK fertilizer was applied. We found that changes in environmental factors can affect leaf quality, indicating a potential interactive effect between land-use changes and environmental changes on savanna vegetation: under more uneven rainfall patterns within the growing season, leaf quality of tree seedlings for a number of species can change as a response to changes in nutrient levels, even if overall plant biomass does not change. Such changes might affect herbivore pressure on trees and thus savanna plant community dynamics. Although longer term experiments would be essential to test such potential effects of eutrophication via changes in leaf nutrient concentration, our findings provide important insights that can help guide management plans that aim to preserve savanna biodiversity.

Seasonal Belowground Ecosystem and Eco-enzymatic Responses to Soil pH and Phosphorus Availability in Temperate Hardwood Forests

NASA Astrophysics Data System (ADS)

Smemo, K. A.; Deforest, J. L.; Petersen, S. L.; Burke, D.; Hewins, C.; Kluber, L. A.; Kyker, S. R.

2013-12-01

Atmospheric acid deposition can increase phosphorus (P) limitation in temperate hardwood forests by increasing N availability, and therefore P demand, and/or by decreasing pH and occluding inorganic P. However, only recently have studies demonstrated that P limitation can occur in temperate forests and very little is known about the temporal aspects of P dynamics in acidic forest soils and how seasonal shifts in nutrient availability and demand influence microbial investment in extracellular enzymes. The objectives of this study were to investigate how P availability and soil pH influence seasonal patterns of nutrient cycling and soil microbial activity in hardwood forests that experience chronic acid deposition. We experimentally manipulated soil pH, P, or both for three years and examined soil treatment responses in fall, winter, spring, early summer, and late summer. We found that site (glaciated versus unglaciated) and treatment had the most significant influence on nutrient pools and cycling. In general, nutrient pools were higher in glaciated soils than unglaciated for measured nutrients, including total C and N (2-3 times higher), extractable inorganic nitrogen, and readily available P. Treatment had no impact on total C and N pools in either region, but did affect other measured nutrients such as ammonium, which was greatest in the elevated pH treatment for both sites. As expected, readily available P pools were highest in the elevated P treatments (3 fold increase in both sites), but raising pH decreased available P pools in the glaciated site. Raising soil pH increased both net N mineralization rates and net P mineralization rates, regardless of site. Nitrification responses were complex, but we observed an overall significant nitrification increase under elevated pH, particularly in the growing season. Extracellular enzyme activity showed more seasonal patterns than site and treatment effects, exhibiting significant growing season activity reductions for all enzymes measured. Phosphatase enzymes did not respond to our treatments and were generally greatest in the unglaciated soils, particularly in winter and spring. Enzyme stoichiometric relationships revealed that soil microbial populations in the glaciated site were consistently less P and N-limited than unglaciated sites but this difference was less pronounced during the growing season. The trajectory of nutrient limitation in response to soil pH and P availability was highly variable, but we observed that enzyme ratios in the early summer were particularly shifted relative to other seasons suggesting that both sites were increasingly P and N-limited during this period. Overall, our results suggest that ecosystem and microbial responses to soil pH and P availability vary with both season and site history and that more spatially and temporally explicit observations are needed to improve our understanding of ecosystem acidification, nutrient limitation, and the cost-benefit relationships of microbial investments in extracellular enzymes.

Rapid Shifts in Soil Nutrients and Decomposition Enzyme Activity in Early Succession Following Forest Fire

DOE PAGES

Knelman, Joseph E.; Graham, Emily B.; Ferrenberg, Scott; ...

2017-09-15

In post-disturbance landscapes nutrient availability has proven a major control on ecological succession. In this study, we examined variation in connections between soil nutrient availability and decomposition extracellular enzyme activity (EEA) across post fire secondary succession in forest soils as well as after a secondary flood disturbance. We also examined possible linkages between edaphic properties and bacterial communities based on 16S rRNA gene analysis. We found that with advancing succession in a post-fire landscape, the relationship between soil nutrients and EEA became stronger over time. In general, late successional soils showed stronger connections between EEA and soil nutrient status, whilemore » early successional soils were marked by a complete decoupling of nutrients and EEA. We also found that soil moisture and bacterial communities of post-fire disturbance soils were susceptible to change following the secondary flood disturbance, while undisturbed, reference forest soils were not. Our results demonstrate that nutrient pools correlating with EEA change over time. While past work has largely focused on ecosystem succession on decadal timescales, our work suggests that nutrients shift in their relative importance as a control of decomposition EEA in the earliest stages of secondary succession. Furthermore, this work emphasizes the relevance of successional stage, even on short timescales, in predicting rates of carbon and nitrogen cycling, especially as disturbances become more frequent in a rapidly changing world.« less

Rapid Shifts in Soil Nutrients and Decomposition Enzyme Activity in Early Succession Following Forest Fire

DOE Office of Scientific and Technical Information (OSTI.GOV)

Knelman, Joseph E.; Graham, Emily B.; Ferrenberg, Scott

In post-disturbance landscapes nutrient availability has proven a major control on ecological succession. In this study, we examined variation in connections between soil nutrient availability and decomposition extracellular enzyme activity (EEA) across post fire secondary succession in forest soils as well as after a secondary flood disturbance. We also examined possible linkages between edaphic properties and bacterial communities based on 16S rRNA gene analysis. We found that with advancing succession in a post-fire landscape, the relationship between soil nutrients and EEA became stronger over time. In general, late successional soils showed stronger connections between EEA and soil nutrient status, whilemore » early successional soils were marked by a complete decoupling of nutrients and EEA. We also found that soil moisture and bacterial communities of post-fire disturbance soils were susceptible to change following the secondary flood disturbance, while undisturbed, reference forest soils were not. Our results demonstrate that nutrient pools correlating with EEA change over time. While past work has largely focused on ecosystem succession on decadal timescales, our work suggests that nutrients shift in their relative importance as a control of decomposition EEA in the earliest stages of secondary succession. Furthermore, this work emphasizes the relevance of successional stage, even on short timescales, in predicting rates of carbon and nitrogen cycling, especially as disturbances become more frequent in a rapidly changing world.« less

HCMM hydrological analysis in Utah

NASA Technical Reports Server (NTRS)

Miller, A. W. (Principal Investigator)

1982-01-01

The feasibility of applying a linear model to HCMM data in hopes of obtaining an accurate linear correlation was investigated. The relationship among HCMM sensed data surface temperature and red reflectivity on Utah Lake and water quality factors including algae concentrations, algae type, and nutrient and turbidity concentrations was established and evaluated. Correlation (composite) images of day infrared and reflectance imagery were assessed to determine if remote sensing offers the capability of using masses of accurate and comprehensive data in calculating evaporation. The effects of algae on temperature and evaporation were studied and the possibility of using satellite thermal data to locate areas within Utah Lake where significant thermal sources exist and areas of near surface groundwater was examined.

Recent Advances in the Cellular and Molecular Mechanisms of Hypothalamic Neuronal Glucose Detection.

PubMed

Fioramonti, Xavier; Chrétien, Chloé; Leloup, Corinne; Pénicaud, Luc

2017-01-01

The hypothalamus have been recognized for decades as one of the major brain centers for the control of energy homeostasis. This area contains specialized neurons able to detect changes in nutrients level. Among them, glucose-sensing neurons use glucose as a signaling molecule in addition to its fueling role. In this review we will describe the different sub-populations of glucose-sensing neurons present in the hypothalamus and highlight their nature in terms of neurotransmitter/neuropeptide expression. This review will particularly discuss whether pro-opiomelanocortin (POMC) neurons from the arcuate nucleus are directly glucose-sensing. In addition, recent observations in glucose-sensing suggest a subtle system with different mechanisms involved in the detection of changes in glucose level and their involvement in specific physiological functions. Several data point out the critical role of reactive oxygen species (ROS) and mitochondria dynamics in the detection of increased glucose. This review will also highlight that ATP-dependent potassium (K ATP ) channels are not the only channels mediating glucose-sensing and discuss the new role of transient receptor potential canonical channels (TRPC). We will discuss the recent advances in the determination of glucose-sensing machinery and propose potential line of research needed to further understand the regulation of brain glucose detection.

Growth, biomass allocation and nutrient use efficiency in Cladium jamaicense and Typha domingensis as affected by phosphorus and oxygen availability

USGS Publications Warehouse

Lorenzen, B.; Brix, H.; Mendelssohn, I.A.; McKee, K.L.; Miao, S.L.

2001-01-01

The effects of phosphorus (P) and oxygen availability on growth, biomass allocation and nutrient use efficiency in Cladium jamaicense Crantz and Typha domingensis Pers. were studied in a growth facility equipped with steady-state hydroponic rhizotrons. The treatments included four P concentrations (10, 40, 80 and 500 ??g I-1) and two oxygen concentration (8.0 and <0.5 mg O2 I-1) in the culture solutions. In Cladium, no clear relationship was found between P availability and growth rate (19-37 mg g-1 d-1), the above to below ground biomass ratio (A/B) (mean = 4.6), or nitrogen use efficiency (NUE) (mean = 72 g dry weight g-1 N). However, the ratio between root supported tissue (leaves, rhizomes and ramets) and root biomass (S/R) (5.6-8) increased with P availability. In contrast, the growth rate (48-89 mg g-1 d-1) and the biomass ratios A/B (2.4-6.1) and S/R (5.4-10.3) of Typha increased with P availability, while NUE (71-30 g dry weight g-1 N) decreased. The proportion of root laterals was similar in the two species, but Typha had thinner root laterals (diameter = 186 ??m) than Cladium (diameter = 438 ??m) indicating a larger root surface area in Typha. The two species had a similar P use efficiency (PUE) at 10 ??g PI-1 (mean = 1134 g dry weight g-1 P) and at 40 and 80 ??g PI-1 (mean = 482 dry weight g-1 P) but the N/P ratio indicated imbalances in nutrient uptake at a higher P concentration (40 ??g PI-1) in Typha than in Cladium (10 ??g PI-1). The two species had similar root specific P accumulation rate at the two lowest P levels, whereas Typha had 3-13-fold higher P uptake rates at the two highest P levels, indicating a higher nutrient uptake capacity in Typha. The experimental oxygen concentration in the rhizosphere had only limited effect on the growth of the two species and had little effect on biomass partitioning and nutrient use efficiency. The aerenchyma in these species was probably sufficient to maintain adequate root oxygenation under partially oxygen depleted conditions. Cladium had characteristics typical for plants from nutrient poor habitats, which included slow growth rate, low capacity for P uptake and relatively inflexible biomass partitioning in response to increased P availability. In contrast, Typha demonstrated a high degree of flexibility in growth, biomass partitioning, and nutrient accumulation to P availability, similar to species from nutrient rich habitats. Although the N/P ratio indicated that Typha was more nutrient stressed at the low P levels, Typha had a higher capacity for P uptake and was more competitive than Cladium at the applied P concentrations. ?? 2001 Elsevier Science B.V.

Proximate and Ultimate Limiting Nutrients in the Mississippi River Plume: Implications for Hypoxia Reduction Through Nutrient Management

NASA Astrophysics Data System (ADS)

Fennel, K.; Laurent, A.

2016-02-01

A large hypoxic area (15,000 km2 on average) forms every summer over the Texas-Louisiana shelf in the northern Gulf of Mexico due to decay of organic matter that is primarily derived from nutrient inputs from the Mississippi/Atchafalaya River System. Efforts are underway to reduce the extent of hypoxic conditions through nutrient management in the watershed; for example, an interagency Hypoxia Task Force is developing Action Plans with input from various stakeholders that set out targets for hypoxia reduction. An open question is by how much nutrient loads would have to be decreased in order to produce the desired reductions in hypoxia and when these would be measurable over natural variability. We have performed a large number of multi-year nutrient load reduction scenarios with a regional biogeochemical model for the region. The model is based on the Regional Ocean Modeling System (ROMS), explicitly includes nitrogen (N) and phosphorus (P) species as inorganic nutrients, and has been shown to realistically reproduce the key processes responsible for hypoxia generation. We have quantified the effects of differential reductions in river N and P loads on hypoxic extent. An assessment of the effects of N versus P reductions is important because, thus far, nutrient management efforts have focused on N, yet P is known to limit primary production in spring and early summer. A debate is ongoing as to whether targets for P reductions should be set and whether nutrient reduction efforts should focus solely on P, which results primarily from urban and industrial point sources and is uncoupled from agricultural fertilizer application. Our results strongly indicate that N is the `ultimate' limiting nutrient to primary production determining the areal extent and duration of hypoxic conditions in a cumulative sense, while P is temporarily limiting in spring. Although reductions in river P load would decrease hypoxic extent in early summer, they would have a much smaller effect than N reductions on the cumulative extent and duration of hypoxic conditions. Combined reductions of N and P have the greatest effect.

Proximate versus ultimate limiting nutrients in the Mississippi River Plume and Implications for Hypoxia Reductions through Nutrient Management

NASA Astrophysics Data System (ADS)

Fennel, Katja; Laurent, Arnaud

2016-04-01

A large hypoxic area (15,000 km2 on average) forms every summer over the Texas-Louisiana shelf in the northern Gulf of Mexico due to decay of organic matter that is primarily derived from nutrient inputs from the Mississippi/Atchafalaya River System. Efforts are underway to reduce the extent of hypoxic conditions through nutrient management in the watershed; for example, an interagency Hypoxia Task Force is developing Action Plans with input from various stakeholders that set out targets for hypoxia reduction. An open question is how far nutrient loads would have to be decreased in order to produce the desired reductions in hypoxia and when these would be measurable given significant natural variability. We have simulated a large number of multi-year nutrient load reduction scenarios with a regional biogeochemical model for the region. The model is based on the Regional Ocean Modeling System (ROMS), explicitly includes nitrogen (N) and phosphorus (P) species as inorganic nutrients, and has been shown to realistically reproduce the key processes responsible for hypoxia generation. We have quantified the effects of differential reductions in river N and P loads on hypoxic extent. An assessment of the effects of N versus P reductions is important because, thus far, nutrient management efforts have focused on N, yet P is known to limit primary production in spring and early summer. A debate is ongoing as to whether targets for P reductions should be set and whether nutrient reduction efforts should focus solely on P, which results primarily from urban and industrial point sources and is uncoupled from agricultural fertilizer application. Our results strongly indicate that N is the 'ultimate' limiting nutrient to primary production determining the areal extent and duration of hypoxic conditions in a cumulative sense, while P is temporarily limiting in spring. Although reductions in river P load would decrease hypoxic extent in early summer, they would have a much smaller effect than N reductions on the cumulative extent and duration of hypoxic conditions. Combined reductions of N and P have the greatest effect.

Growth of Myxococcus xanthus in continuous-flow-cell bioreactors as a method for studying development.

PubMed

Smaldone, Gregory T; Jin, Yujie; Whitfield, Damion L; Mu, Andrew Y; Wong, Edward C; Wuertz, Stefan; Singer, Mitchell

2014-04-01

Nutrient sensors and developmental timers are two classes of genes vital to the establishment of early development in the social soil bacterium Myxococcus xanthus. The products of these genes trigger and regulate the earliest events that drive the colony from a vegetative state to aggregates, which ultimately leads to the formation of fruiting bodies and the cellular differentiation of the individual cells. In order to more accurately identify the genes and pathways involved in the initiation of this multicellular developmental program in M. xanthus, we adapted a method of growing vegetative populations within a constant controllable environment by using flow cell bioreactors, or flow cells. By establishing an M. xanthus community within a flow cell, we are able to test developmental responses to changes in the environment with fewer concerns for effects due to nutrient depletion or bacterial waste production. This approach allows for greater sensitivity in investigating communal environmental responses, such as nutrient sensing. To demonstrate the versatility of our growth environment, we carried out time-lapse confocal laser scanning microscopy to visualize M. xanthus biofilm growth and fruiting body development, as well as fluorescence staining of exopolysaccharides deposited by biofilms. We also employed the flow cells in a nutrient titration to determine the minimum concentration required to sustain vegetative growth. Our data show that by using a flow cell, M. xanthus can be held in a vegetative growth state at low nutrient concentrations for long periods, and then, by slightly decreasing the nutrient concentration, cells can be allowed to initiate the developmental program.

Response diversity, nonnative species, and disassembly rules buffer freshwater ecosystem processes from anthropogenic change.

PubMed

Moore, Jonathan W; Olden, Julian D

2017-05-01

Integrating knowledge of environmental degradation, biodiversity change, and ecosystem processes across large spatial scales remains a key challenge to illuminating the resilience of earth's systems. There is now a growing realization that the manner in which communities will respond to anthropogenic impacts will ultimately control the ecosystem consequences. Here, we examine the response of freshwater fishes and their nutrient excretion - a key ecosystem process that can control aquatic productivity - to human land development across the contiguous United States. By linking a continental-scale dataset of 533 fish species from 8100 stream locations with species functional traits, nutrient excretion, and land remote sensing, we present four key findings. First, we provide the first geographic footprint of nutrient excretion by freshwater fishes across the United States and reveal distinct local- and continental-scale heterogeneity in community excretion rates. Second, fish species exhibited substantial response diversity in their sensitivity to land development; for native species, the more tolerant species were also the species contributing greater ecosystem function in terms of nutrient excretion. Third, by modeling increased land-use change and resultant shifts in fish community composition, land development is estimated to decrease fish nutrient excretion in the majority (63%) of ecoregions. Fourth, the loss of nutrient excretion would be 28% greater if biodiversity loss was random or 84% greater if there were no nonnative species. Thus, ecosystem processes are sensitive to increased anthropogenic degradation but biotic communities provide multiple pathways for resistance and this resistance varies across space. © 2016 John Wiley & Sons Ltd.

Synthetic Spores Give Insight into the Real Thing and Reveal Functional Applications | Center for Cancer Research

Cancer.gov

Spores from bacteria, such as Bacillus subtilis, are produced to allow the bacterium’s genetic material to survive harsh environments. When the bacterium senses nutrient depletion, it divides asymmetrically into a forespore and a mother cell. The mother cell engulfs the forespore, and coat proteins synthesized by the mother cell localize to the surface of the forespore. The

DOE Office of Scientific and Technical Information (OSTI.GOV)

Takeda-Watanabe, Ai; Kitada, Munehiro; Kanasaki, Keizo

Highlights: Black-Right-Pointing-Pointer SIRT1 inactivation decreases autophagy in THP-1 cell. Black-Right-Pointing-Pointer Inhibition of autophagy induces inflammation. Black-Right-Pointing-Pointer SIRT1 inactivation induces inflammation through NF-{kappa}B activation. Black-Right-Pointing-Pointer The p62/Sqstm1 accumulation by impairment of autophagy is related to NF-{kappa}B activation. Black-Right-Pointing-Pointer SIRT1 inactivation is involved in the activation of mTOR and decreased AMPK activation. -- Abstract: Inflammation plays a crucial role in atherosclerosis. Monocytes/macrophages are some of the cells involved in the inflammatory process in atherogenesis. Autophagy exerts a protective effect against cellular stresses like inflammation, and it is regulated by nutrient-sensing pathways. The nutrient-sensing pathway includes SIRT1, a NAD{sup +}-dependent histone deacetylase, whichmore » is implicated in the regulation of a variety of cellular processes including inflammation and autophagy. The mechanism through which the dysfunction of SIRT1 contributes to the regulation of inflammation in relation to autophagy in monocytes/macrophages is unclear. In the present study, we demonstrate that treatment with 2-[(2-Hydroxynaphthalen-1-ylmethylene)amino]-N-(1-phenethyl)benzamide (Sirtinol), a chemical inhibitor of SIRT1, induces the overexpression of inflammation-related genes such as tumor necrosis factor (TNF)-{alpha} and interleukin (IL)-6 through nuclear factor (NF)-{kappa}B signaling activation, which is associated with autophagy dysfunction, as shown through p62/Sqstm1 accumulation and decreased expression of light chain (LC) 3 II in THP-1 cells. The autophagy inhibitor, 3-methyladenine, also induces inflammation-related NF-{kappa}B activation. In p62/Sqstm1 knockdown cells, Sirtinol-induced inflammation through NF-{kappa}B activation is blocked. In addition, inhibition of SIRT1 is involved in the activation of the mammalian target of rapamycin (mTOR) pathway and is implicated in decreased 5 Prime -AMP activated kinase (AMPK) activation, leading to the impairment of autophagy. The mTOR inhibitor, rapamycin, abolishes Sirtinol-induced inflammation and NF-{kappa}B activation associated with p62/Sqstm1 accumulation. In summary, SIRT1 inactivation induces inflammation through NF-{kappa}B activation and dysregulates autophagy via nutrient-sensing pathways such as the mTOR and AMPK pathways, in THP-1 cells.« less

Effects of thinning, residue mastication, and prescribed fire on soil and nutrient budgets in a Sierra Nevada mixed-conifer forest

USDA-ARS?s Scientific Manuscript database

The effects of thinning followed by residue mastication (THIN), prescribed fire (BURN), and thinning plus residue mastication plus burning (T+B) on nutrient budgets and resin-based (plant root simulator [PRS] probe) measurements of soil nutrient availability in a mixed-conifer forest were measured. ...

Effects of thinning, residue mastication, and prescribed fire on soil and nutrient budgets in a Sierra Nevada mixed conifer forest

USDA-ARS?s Scientific Manuscript database

The effects of thinning followed by residue mastication (THIN), prescribed fire (BURN), and thinning plus residue mastication plus burning (T+B) on nutrient budgets and resin-based (plant root simulator [PRS] probe) measurements of soil nutrient availability in a mixed-conifer forest were measured. ...

Characterization of Pseudomonas putida Genes Responsive to Nutrient Limitation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Syn, Chris K.; Magnuson, Jon K.; Kingsley, Mark T.

2004-06-01

The low bioavailability of nutrients and oxygen in the soil environment has hampered successful expression of biodegradation/biocontrol genes that are driven by promoters highly active during routine laboratory conditions of high nutrient- and oxygen-availability. Hence, in the present study, expression of the gus-tagged genes in 12 Tn5-gus mutants of the soil microbe Pseudomonas putida PNL-MK25 was examined under various conditions chosen to mimic the soil environment: low carbon, phosphate, nitrate, or oxygen, and in the rhizosphere. Based on their expression profiles, three nutrient-responsive mutant (NRM) strains, NRM5, NRM7, and NRM17, were selected for identification of the tagged genes. In themore » mutant strain NRM5, expression of the glutamate dehydrogenase (gdhA) gene was increased between 4.9- to 26.4-fold under various low nutrient conditions. In NRM7, expression of the novel NADPH:quinone oxidoreductase-like (nql) gene was consistently amongst the highest and was synergistically upregulated by low nutrient and anoxic conditions. The cyoD gene in NRM17, which encodes the fourth subunit of the cytochrome o ubiquinol oxidase complex, had decreased expression in low nutrient conditions but its absolute expression levels was still amongst the highest. Additionally, it was independent of oxygen availability, in contrast to that in E. coli.« less

Altered resource availability and the population dynamics of tree species in Amazonian secondary forests.

PubMed

Fortini, Lucas Berio; Bruna, Emilio M; Zarin, Daniel J; Vasconcelos, Steel S; Miranda, Izildinha S

2010-04-01

Despite research demonstrating that water and nutrient availability exert strong effects on multiple ecosystem processes in tropical forests, little is known about the effect of these factors on the demography and population dynamics of tropical trees. Over the course of 5 years, we monitored two common Amazonian secondary forest species-Lacistema pubescens and Myrcia sylvatica-in dry-season irrigation, litter-removal and control plots. We then evaluated the effects of altered water and nutrient availability on population demography and dynamics using matrix models and life table response experiments. Our results show that despite prolonged experimental manipulation of water and nutrient availability, there were nearly no consistent and unidirectional treatment effects on the demography of either species. The patterns and significance of observed treatment effects were largely dependent on cross-year variability not related to rainfall patterns, and disappeared once we pooled data across years. Furthermore, most of these transient treatment effects had little effect on population growth rates. Our results suggest that despite major experimental manipulations of water and nutrient availability-factors considered critical to the ecology of tropical pioneer tree species-autogenic light limitation appears to be the primary regulator of tree demography at early/mid successional stages. Indeed, the effects of light availability may completely override those of other factors thought to influence the successional development of Amazonian secondary forests.

Nutrient requirements and low-cost balanced diets, based on seasonally available local feedstuffs, for local pigs on smallholder farms in Western Kenya.

PubMed

Carter, Natalie Ann; Dewey, Catherine Elizabeth; Thomas, Lian Francesca; Lukuyu, Ben; Grace, Delia; de Lange, Cornelis

2016-02-01

Growth performance of pigs on smallholder farms in the tropics is low. Lack of feedstuffs, seasonal feed shortages, and feeding nutritionally unbalanced diets contribute to slow growth. Low-cost balanced diets are needed to improve pig performance. In this study, we estimated the nutrient requirements of local pigs on smallholder farms in Kenya and developed balanced low-cost diets using seasonally available local feedstuffs. Diets were formulated to provide pigs with 80 % of the nutrient density in corn and soybean meal-based (reference) diets to minimize the cost per unit of energy and other nutrients. Estimated requirements for starting and growing pigs (8 to 35 kg body weight) were as follows: digestible energy (DE) 2960 kcal/kg of dry matter (DM), standardized ileal digestibility (SID) lysine 5.8 g/kg of DM, calcium 2.8 g/kg of DM, standardized total tract digestible (STTD) phosphorous 1.4 g/kg of DM, and crude protein 85 g/kg of DM. Nutrient requirements of local pigs on smallholder farms in Kenya were lower than those of exotic breed pigs raised in commercial settings. Seasonally available local feedstuffs were used to develop low-cost balanced diets. Twenty-two diets are presented based on season, cost, and feedstuff availability. This study has broad applicability as a case study of an approach that could be applied in other tropical regions in which smallholder pig keeping is practiced and where local feedstuffs for pigs are available seasonally.

Plant allocation of carbon to defense as a function of herbivory, light and nutrient availability

USGS Publications Warehouse

DeAngelis, Donald L.; Ju, Shu; Liu, Rongsong; Bryant, John P.; Gourley, Stephen A.

2012-01-01

We use modeling to determine the optimal relative plant carbon allocations between foliage, fine roots, anti-herbivore defense, and reproduction to maximize reproductive output. The model treats these plant components and the herbivore compartment as variables. Herbivory is assumed to be purely folivory. Key external factors include nutrient availability, degree of shading, and intensity of herbivory. Three alternative functional responses are used for herbivory, two of which are variations on donor-dependent herbivore (models 1a and 1b) and one of which is a Lotka–Volterra type of interaction (model 2). All three were modified to include the negative effect of chemical defenses on the herbivore. Analysis showed that, for all three models, two stable equilibria could occur, which differs from most common functional responses when no plant defense component is included. Optimal strategies of carbon allocation were defined as the maximum biomass of reproductive propagules produced per unit time, and found to vary with changes in external factors. Increased intensity of herbivory always led to an increase in the fractional allocation of carbon to defense. Decreases in available limiting nutrient generally led to increasing importance of defense. Decreases in available light had little effect on defense but led to increased allocation to foliage. Decreases in limiting nutrient and available light led to decreases in allocation to reproduction in models 1a and 1b but not model 2. Increases in allocation to plant defense were usually accompanied by shifts in carbon allocation away from fine roots, possibly because higher plant defense reduced the loss of nutrients to herbivory.

Characterization of soil spatial variability for site-specific management using soil electrical conductivity and other remotely sensed data

NASA Astrophysics Data System (ADS)

Bang, Jisu

Field-scale characterization of soil spatial variability using remote sensing technology has potential for achieving the successful implementation of site-specific management (SSM). The objectives of this study were to: (i) examine the spatial relationships between apparent soil electrical conductivity (EC a) and soil chemical and physical properties to determine if EC a could be useful to characterize soil properties related to crop productivity in the Coastal Plain and Piedmont of North Carolina; (ii) evaluate the effects of in-situ soil moisture variation on ECa mapping as a basis for characterization of soil spatial variability and as a data layer in cluster analysis as a means of delineating sampling zones; (iii) evaluate clustering approaches using different variable sets for management zone delineation to characterize spatial variability in soil nutrient levels and crop yields. Studies were conducted in two fields in the Piedmont and three fields in the Coastal Plain of North Carolina. Spatial measurements of ECa via electromagnetic induction (EMI) were compared with soil chemical parameters (extractable P, K, and micronutrients; pH, cation exchange capacity [CEC], humic matter or soil organic matter; and physical parameters (percentage sand, silt, and clay; and plant-available water [PAW] content; bulk density; cone index; saturated hydraulic conductivity [Ksat] in one of the coastal plain fields) using correlation analysis across fields. We also collected ECa measurements in one coastal plain field on four days with significantly different naturally occurring soil moisture conditions measured in five increments to 0.75 m using profiling time-domain reflectometry probes to evaluate the temporal variability of ECa associated with changes in in-situ soil moisture content. Nonhierarchical k-means cluster analysis using sensor-based field attributes including vertical ECa, near-infrared (NIR) radiance of bare-soil from an aerial color infrared (CIR) image, elevation, slope, and their combinations was performed to delineate management zones. The strengths and signs of the correlations between ECa and measured soil properties varied among fields. Few strong direct correlations were found between ECa and the soil chemical and physical properties studied (r2 < 0.50), but correlations improved considerably when zone mean ECa and zone means of selected soil properties among ECa zones were compared. The results suggested that field-scale ECa survey is not able to directly predict soil nutrient levels at any specific location, but could delimit distinct zones of soil condition among which soil nutrient levels differ, providing an effective basis for soil sampling on a zone basis. (Abstract shortened by UMI.)

The regulation of K- and L-cell activity by GLUT2 and the calcium-sensing receptor CasR in rat small intestine

PubMed Central

Mace, Oliver J; Schindler, Marcus; Patel, Sonal

2012-01-01

Intestinal enteroendocrine cells (IECs) secrete gut peptides in response to both nutrients and non-nutrients. Glucose and amino acids both stimulate gut peptide secretion. Our hypothesis was that the facilitative glucose transporter, GLUT2, could act as a glucose sensor and the calcium-sensing receptor, CasR, could detect amino acids in the intestine to modify gut peptide secretion. We used isolated loops of rat small intestine to study the secretion of gluco-insulinotropic peptide (GIP), glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) secretion stimulated by luminal perfusion of nutrients or bile acid. Inhibition of the sodium-dependent glucose cotransporter 1 (SGLT1) with phloridzin partially inhibited GIP, GLP-1 and PYY secretion by 45%, suggesting another glucose sensor might be involved in modulating peptide secretion. The response was completely abolished in the presence of the GLUT2 inhibitors phloretin or cytochalasin B. Given that GLUT2 modified gut peptide secretion stimulated by glucose, we investigated whether it was involved in the secretion of gut peptide by other gut peptide secretagogues. Phloretin completely abolished gut peptide secretion stimulated by artificial sweetener (sucralose), dipeptide (glycylsarcosine), lipid (oleoylethanolamine), short chain fatty acid (propionate) and major rat bile acid (taurocholate) indicating a fundamental position for GLUT2 in the gut peptide secretory mechanism. We investigated how GLUT2 was able to influence gut peptide secretion mediated by a diverse range of stimulators and discovered that GLUT2 affected membrane depolarisation through the closure of K+ATP-sensitive channels. In the absence of SGLT1 activity (or presence of phloridzin), the secretion of GIP, GLP-1 and PYY was sensitive to K+ATP-sensitive channel modulators tolbutamide and diazoxide. l-Amino acids phenylalanine (Phe), tryptophan (Trp), asparagine (Asn), arginine (Arg) and glutamine (Gln) also stimulated GIP, GLP-1 and PYY secretion, which was completely abolished when extracellular Ca2+ was absent. The gut peptide response stimulated by the amino acids was also blocked by the CasR inhibitor Calhex 231 and augmented by the CasR agonist NPS-R568. GLUT2 and CasR regulate K- and L-cell activity in response to nutrient and non-nutrient stimuli. PMID:22495587

Effects of feeding frequency on apparent energy and nutrient digestibility/availability of channel catfish, Ictalurus punctatus, reared at optimal and suboptimal temperatures

USDA-ARS?s Scientific Manuscript database

This study examined the effects of feeding frequency (daily versus every other day [EOD]) on nutrient digestibility/availability of channel catfish, Ictalurus punctatus, reared at optimal (30 C) and suboptimal (24 C) temperatures. A 28% protein practical diet was used as the test diet, and chromic o...

Effects of long-term nutrient additions on Arctic tundra, stream, and lake ecosystems: beyond NPP.

PubMed

Gough, Laura; Bettez, Neil D; Slavik, Karie A; Bowden, William B; Giblin, Anne E; Kling, George W; Laundre, James A; Shaver, Gaius R

2016-11-01

Primary producers form the base of food webs but also affect other ecosystem characteristics, such as habitat structure, light availability, and microclimate. Here, we examine changes caused by 5-30+ years of nutrient addition and resulting increases in net primary productivity (NPP) in tundra, streams, and lakes in northern Alaska. The Arctic provides an important opportunity to examine how ecosystems characterized by low diversity and low productivity respond to release from nutrient limitation. We review how responses of algae and plants affect light availability, perennial biotic structures available for consumers, oxygen levels, and temperature. Sometimes, responses were similar across all three ecosystems; e.g., increased NPP significantly reduced light to the substrate following fertilization. Perennial biotic structures increased in tundra and streams but not in lakes, and provided important new habitat niches for consumers as well as other producers. Oxygen and temperature responses also differed. Life history traits (e.g., longevity) of the primary producers along with the fate of detritus drove the responses and recovery. As global change persists and nutrients become more available in the Arctic and elsewhere, incorporating these factors as response variables will enable better prediction of ecosystem changes and feedbacks in this biome and others.

Unmanned aerial systems for forest reclamation monitoring: throwing balloons in the air

NASA Astrophysics Data System (ADS)

Andrade, Rita; Vaz, Eric; Panagopoulos, Thomas; Guerrero, Carlos

2014-05-01

Wildfires are a recurrent phenomenon in Mediterranean landscapes, deteriorating environment and ecosystems, calling out for adequate land management. Monitoring burned areas enhances our abilities to reclaim them. Remote sensing has become an increasingly important tool for environmental assessment and land management. It is fast, non-intrusive, and provides continuous spatial coverage. This paper reviews remote sensing methods, based on space-borne, airborne or ground-based multispectral imagery, for monitoring the biophysical properties of forest areas for site specific management. The usage of satellite imagery for land use management has been frequent in the last decades, it is of great use to determine plants health and crop conditions, allowing a synergy between the complexity of environment, anthropogenic landscapes and multi-temporal understanding of spatial dynamics. Aerial photography increments on spatial resolution, nevertheless it is heavily dependent on airborne availability as well as cost. Both these methods are required for wide areas management and policy planning. Comprising an active and high resolution imagery source, that can be brought at a specific instance, reducing cost while maintaining locational flexibility is of utmost importance for local management. In this sense, unmanned aerial vehicles provide maximum flexibility with image collection, they can incorporate thermal and multispectral sensors, however payload and engine operation time limit flight time. Balloon remote sensing is becoming increasingly sought after for site specific management, catering rapid digital analysis, permitting greater control of the spatial resolution as well as of datasets collection in a given time. Different wavelength sensors may be used to map spectral variations in plant growth, monitor water and nutrient stress, assess yield and plant vitality during different stages of development. Proximity could be an asset when monitoring forest plants vitality. Early predictions of re-vegetation success facilitate precise and timely diagnosis of stress, thus remedial actions can be taken at localized detail.

Geostatistical approach for management of soil nutrients with special emphasis on different forms of potassium considering their spatial variation in intensive cropping system of West Bengal, India.

PubMed

Chatterjee, Sourov; Santra, Priyabrata; Majumdar, Kaushik; Ghosh, Debjani; Das, Indranil; Sanyal, S K

2015-04-01

A large part of precision agriculture research in the developing countries is devoted towards precision nutrient management aspects. This has led to better economics and efficiency of nutrient use with off-farm advantages of environmental security. The keystone of precision nutrient management is analysis and interpretation of spatial variability of soils by establishing management zones. In this study, spatial variability of major soil nutrient contents was evaluated in the Ghoragacha village of North 24 Parganas district of West Bengal, India. Surface soil samples from 100 locations, covering different cropping systems of the village, was collected from 0 to 15 cm depth using 100×100 m grid system and analyzed in the laboratory to determine organic carbon (OC), available nitrogen (N), phosphorus (P), and potassium (K) contents of the soil as well as its water-soluble K (KWS), exchangeable K (KEX), and non-exchangeable forms of K (KNEX). Geostatistical analyses were performed to determine the spatial variation structure of each nutrient content within the village, followed by the generation of surface maps through kriging. Four commonly used semivariogram models, i.e., spherical, exponential, Gaussian, and linear models were fitted to each soil property, and the best one was used to prepare surface maps through krigging. Spherical model was found the best for available N and P contents, while linear and exponential model was the best for OC and available K, and for KWS and KNEK, Gausian model was the best. Surface maps of nutrient contents showed that N content (129-195 kg ha(-1)) was the most limiting factor throughout the village, while P status was generally very high ( 10-678 kg ha(-1)) in the soils of the present village. Among the different soil K fractions, KWS registered the maximum variability (CV 75%), while the remaining soil K fractions showed moderate to high variation. Interestingly, KNEX content also showed high variability, which essentially indicates reserve native K exploitation under intensive cultivation. These maps highlight the necessity of estimating the other soil K fractions as well for better understanding of soil K supplying capacity and K fertilization strategy rather than the current recommendations, based on the plant-available K alone. In conclusion, the present study revealed that the variability of nutrient distribution was a consequence of complex interactions between the cropping system, nutrient application rates, and the native soil characteristics, and such interactions could be utilized to develop the nutrient management strategies for intensive small-holder system.

Carbohydrate and lipid spectroscopic molecular structures of different alfalfa hay and their relationship with nutrient availability in ruminants

PubMed Central

Yari, Mojtaba; Valizadeh, Reza; Nnaserian, Abbas Ali; Jonker, Arjan; Yu, Peiqiang

2017-01-01

Objective This study was conducted to determine molecular structures related to carbohydrates and lipid in alfalfa hay cut at early bud, late bud and early flower and in the afternoon and next morning using Fourier transform infrared spectroscopy (FT/IR) and to determine their relationship with alfalfa hay nutrient profile and availability in ruminants. Methods Chemical composition analysis, carbohydrate fractionation, in situ ruminal degradability, and DVE/OEB model were used to measure nutrient profile and availability of alfalfa hay. Univariate analysis, hierarchical cluster analysis (CLA) and principal components analysis (PCA) were conducted to identify FT/IR spectra differences. Results The FT/IR non-structural carbohydrate (NSCHO) to total carbohydrates and NSCHO to structural carbohydrate ratios decreased (p<0.05), while lignin to NSCHO and lipid CH3 symmetric to CH2 symmetric ratios increased with advancing maturity (p<0.05). The FT/IR spectra related to structural carbohydrates, lignin and lipids were distinguished for alfalfa hay at three maturities by PCA and CLA, while FT/IR molecular structures related to carbohydrates and lipids were similar between alfalfa hay cut in the morning and afternoon when analyzed by PCA and CLA analysis. Positive correlations were found for FT/IR NSCHO to total carbohydrate and NSCHO to structural carbohydrate ratios with non-fiber carbohydrate (by wet chemistry), ruminal fast and intermediately degradable carbohydrate fractions and total ruminal degradability of carbohydrates and predicted intestinal nutrient availability in dairy cows (r≥0.60; p<0.05) whereas FT/IR lignin to NSCHO and CH3 to CH2 symmetric stretching ratio had negative correlation with predicted ruminal and intestinal nutrient availability of alfalfa hay in dairy cows (r≥−0.60; p<0.05). Conclusion FT/IR carbohydrate and lipid molecular structures in alfalfa hay changed with advancing maturity from early bud to early flower, but not during the day, and these molecular structures correlated with predicted nutrient supply of alfalfa hay in ruminants. PMID:28335093

Arctic water tracks retain phosphorus and transport ammonium

NASA Astrophysics Data System (ADS)

Harms, T.; Cook, C. L.; Wlostowski, A. N.; Godsey, S.; Gooseff, M. N.

2017-12-01

Hydrologic flowpaths propagate biogeochemical signals among adjacent ecosystems, but reactions may attenuate signals by retaining, removing, or transforming dissolved and suspended materials. The theory of nutrient spiraling describes these simultaneous reaction and transport processes, but its application has been limited to stream channels. We applied nutrient spiraling theory to water tracks, zero-order channels draining Arctic hillslopes that contain perennially saturated soils and flow at the surface either perennially or in response to precipitation. In the Arctic, experimental warming results in increased availability of nitrogen, the limiting nutrient for hillslope vegetation at the study site, which may be delivered to aquatic ecosystems by water tracks. Increased intensity of rain events, deeper snowpack, earlier snowmelt, and increasing thaw depth resulting from climate change might support increased transport of nutrients, but the reactive capacity of hillslope flowpaths, including sorption and uptake by plants and microbes, could counter transport to regulate solute flux. Characteristics of flowpaths might influence the opportunity for reaction, where slower flowpaths increase the contact time between solutes and soils or roots. We measured nitrogen and phosphorus uptake and transient storage of water tracks through the growing season and found that water tracks retain inorganic phosphorus, but transport ammonium. Nutrient uptake was unrelated to transient storage, suggesting high capacity for nutrient retention by shallow organic soils and vegetation. These observations indicate that increased availability of ammonium, the biogeochemical signal of warming tundra, is propagated by hillslope flowpaths, whereas water tracks attenuate delivery of phosphorus to aquatic ecosystems, where its availability typically limits production.

Variation in wood nutrients along a tropical soil fertility gradient.

PubMed

Heineman, Katherine D; Turner, Benjamin L; Dalling, James W

2016-07-01

Wood contains the majority of the nutrients in tropical trees, yet controls over wood nutrient concentrations and their function are poorly understood. We measured wood nutrient concentrations in 106 tree species in 10 forest plots spanning a regional fertility gradient in Panama. For a subset of species, we quantified foliar nutrients and wood density to test whether wood nutrients scale with foliar nutrients at the species level, or wood nutrient storage increases with wood density as predicted by the wood economics spectrum. Wood nutrient concentrations varied enormously among species from fourfold in nitrogen (N) to > 30-fold in calcium (Ca), potassium (K), magnesium (Mg) and phosphorus (P). Community-weighted mean wood nutrient concentrations correlated positively with soil Ca, K, Mg and P concentrations. Wood nutrients scaled positively with leaf nutrients, supporting the hypothesis that nutrient allocation is conserved across plant organs. Wood P was most sensitive to variation in soil nutrient availability, and significant radial declines in wood P indicated that tropical trees retranslocate P as sapwood transitions to heartwood. Wood P decreased with increasing wood density, suggesting that low wood P and dense wood are traits associated with tree species persistence on low fertility soils. Substantial variation among species and communities in wood nutrient concentrations suggests that allocation of nutrients to wood, especially P, influences species distributions and nutrient dynamics in tropical forests. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

Computational Investigation of Environment-Noise Interaction in Single-Cell Organisms: The Merit of Expression Stochasticity Depends on the Quality of Environmental Fluctuations.

PubMed

Lück, Anja; Klimmasch, Lukas; Großmann, Peter; Germerodt, Sebastian; Kaleta, Christoph

2018-01-10

Organisms need to adapt to changing environments and they do so by using a broad spectrum of strategies. These strategies include finding the right balance between expressing genes before or when they are needed, and adjusting the degree of noise inherent in gene expression. We investigated the interplay between different nutritional environments and the inhabiting organisms' metabolic and genetic adaptations by applying an evolutionary algorithm to an agent-based model of a concise bacterial metabolism. Our results show that constant environments and rapidly fluctuating environments produce similar adaptations in the organisms, making the predictability of the environment a major factor in determining optimal adaptation. We show that exploitation of expression noise occurs only in some types of fluctuating environment and is strongly dependent on the quality and availability of nutrients: stochasticity is generally detrimental in fluctuating environments and beneficial only at equal periods of nutrient availability and above a threshold environmental richness. Moreover, depending on the availability and nutritional value of nutrients, nutrient-dependent and stochastic expression are both strategies used to deal with environmental changes. Overall, we comprehensively characterize the interplay between the quality and periodicity of an environment and the resulting optimal deterministic and stochastic regulation strategies of nutrient-catabolizing pathways.

Nematodes enhance plant growth and nutrient uptake under C and N-rich conditions.

PubMed

Gebremikael, Mesfin T; Steel, Hanne; Buchan, David; Bert, Wim; De Neve, Stefaan

2016-09-08

The role of soil fauna in crucial ecosystem services such as nutrient cycling remains poorly quantified, mainly because of the overly reductionistic approach adopted in most experimental studies. Given that increasing nitrogen inputs in various ecosystems influence the structure and functioning of soil microbes and the activity of fauna, we aimed to quantify the role of the entire soil nematode community in nutrient mineralization in an experimental set-up emulating nutrient-rich field conditions and accounting for crucial interactions amongst the soil microbial communities and plants. To this end, we reconstructed a complex soil foodweb in mesocosms that comprised largely undisturbed native microflora and the entire nematode community added into defaunated soil, planted with Lolium perenne as a model plant, and amended with fresh grass-clover residues. We determined N and P availability and plant uptake, plant biomass and abundance and structure of the microbial and nematode communities during a three-month incubation. The presence of nematodes significantly increased plant biomass production (+9%), net N (+25%) and net P (+23%) availability compared to their absence, demonstrating that nematodes link below- and above-ground processes, primarily through increasing nutrient availability. The experimental set-up presented allows to realistically quantify the crucial ecosystem services provided by the soil biota.

Nematodes enhance plant growth and nutrient uptake under C and N-rich conditions

NASA Astrophysics Data System (ADS)

Gebremikael, Mesfin T.; Steel, Hanne; Buchan, David; Bert, Wim; de Neve, Stefaan

2016-09-01

The role of soil fauna in crucial ecosystem services such as nutrient cycling remains poorly quantified, mainly because of the overly reductionistic approach adopted in most experimental studies. Given that increasing nitrogen inputs in various ecosystems influence the structure and functioning of soil microbes and the activity of fauna, we aimed to quantify the role of the entire soil nematode community in nutrient mineralization in an experimental set-up emulating nutrient-rich field conditions and accounting for crucial interactions amongst the soil microbial communities and plants. To this end, we reconstructed a complex soil foodweb in mesocosms that comprised largely undisturbed native microflora and the entire nematode community added into defaunated soil, planted with Lolium perenne as a model plant, and amended with fresh grass-clover residues. We determined N and P availability and plant uptake, plant biomass and abundance and structure of the microbial and nematode communities during a three-month incubation. The presence of nematodes significantly increased plant biomass production (+9%), net N (+25%) and net P (+23%) availability compared to their absence, demonstrating that nematodes link below- and above-ground processes, primarily through increasing nutrient availability. The experimental set-up presented allows to realistically quantify the crucial ecosystem services provided by the soil biota.

Effects of nutrient addition on leaf chemistry, morphology, and photosynthetic capacity of three bog shrubs

Treesearch

Jill L. Bubier; Rose Smith; Sari Juutinen; Tim R. Moore; Rakesh Minocha; Stephanie Long; Subash Minocha

2011-01-01

Plants in nutrient-poor environments typically have low foliar nitrogen (N) concentrations, long-lived tissues with leaf traits designed to use nutrients efficiently, and low rates of photosynthesis. We postulated that increasing N availability due to atmospheric deposition would increase photosynthetic capacity, foliar N, and specific leaf area (SLA) of bog shrubs. We...

Food Availability in School Stores in Seoul, South Korea after Implementation of Food- and Nutrient-Based Policies

ERIC Educational Resources Information Center

Choi, Seul Ki; Frongillo, Edward A.; Blake, Christine E.; Thrasher, James F.

2017-01-01

Background: To improve school store food environments, the South Korean government implemented 2 policies restricting unhealthy food sales in school stores. A food-based policy enacted in 2007 restricts specific food sales (soft drinks); and a nutrient-based policy enacted in 2009 restricts energy-dense and nutrient-poor (EDNP) food sales. The…

Evaluation of growth, nutrient retention, health, and resistance to bacterial challenge in sunshine bass fed diets with new varieties of non-genetically modified soybeans

USDA-ARS?s Scientific Manuscript database

We evaluated the effects of meals made from new strains of soybeans with high protein and reduced anti-nutritional factors (ANFs) on hybrid striped bass ("Sunshine bass", Morone chrysops x M. saxatilis) nutrient availability, growth rates, nutrient retention, gut histology, non-specific immune respo...

Plant and soil nutrients in young versus mature central Appalachian hardwood stands

Treesearch

Frank S. Gilliam; Mary Beth Adams

1995-01-01

Most models of forest succession and forest recovery following disturbance predict changes in nutrient availability. The purpose of this study was to compare soil and herbaceous layer plant nutrients between two young (~20 yr) and two mature (~80 yr) forest stands on Fernow Experimental Forest, Parsons, West Virginia. All sampling was carried out within 15 circular 0....

Scale-location specific relations between soil nutrients and topographic factors in the Fen River Basin, Chinese Loess Plateau

NASA Astrophysics Data System (ADS)

Zhu, Hongfen; Bi, Rutian; Duan, Yonghong; Xu, Zhanjun

2017-06-01

Understanding scale- and location-specific variations of soil nutrients in cultivated land is a crucial consideration for managing agriculture and natural resources effectively. In the present study, wavelet coherency was used to reveal the scale-location specific correlations between soil nutrients, including soil organic matter (SOM), total nitrogen (TN), available phosphorus (AP), and available potassium (AK), as well as topographic factors (elevation, slope, aspect, and wetness index) in the cultivated land of the Fen River Basin in Shanxi Province, China. The results showed that SOM, TN, AP, and AK were significantly inter-correlated, and that the scales at which soil nutrients were correlated differed in different landscapes, and were generally smaller in topographically rougher terrain. All soil nutrients but TN were significantly influenced by the wetness index at relatively large scales (32-72 km) and AK was significantly affected by the aspect at large scales at partial locations, showing localized features. The results of this study imply that the wetness index should be taken into account during farming practices to improve the soil nutrients of cultivated land in the Fen River Basin at large scales.

Parasite infection alters nitrogen cycling at the ecosystem scale.

PubMed

Mischler, John; Johnson, Pieter T J; McKenzie, Valerie J; Townsend, Alan R

2016-05-01

Despite growing evidence that parasites often alter nutrient flows through their hosts and can comprise a substantial amount of biomass in many systems, whether endemic parasites influence ecosystem nutrient cycling, and which nutrient pathways may be important, remains conjectural. A framework to evaluate how endemic parasites alter nutrient cycling across varied ecosystems requires an understanding of the following: (i) parasite effects on host nutrient excretion; (ii) ecosystem nutrient limitation; (iii) effects of parasite abundance, host density, host functional role and host excretion rate on nutrient flows; and (iv) how this infection-induced nutrient flux compares to other pools and fluxes. Pathogens that significantly increase the availability of a limiting nutrient within an ecosystem should produce a measurable ecosystem-scale response. Here, we combined field-derived estimates of trematode parasite infections in aquatic snails with measurements of snail excretion and tissue stoichiometry to show that parasites are capable of altering nutrient excretion in their intermediate host snails (dominant grazers). We integrated laboratory measurements of host nitrogen excretion with field-based estimates of infection in an ecosystem model and compared these fluxes to other pools and fluxes of nitrogen as measured in the field. Eighteen nitrogen-limited ponds were examined to determine whether infection had a measurable effect on ecosystem-scale nitrogen cycling. Because of their low nitrogen content and high demand for host carbon, parasites accelerated the rate at which infected hosts excreted nitrogen to the water column in a dose-response manner, thereby shifting nutrient stoichiometry and availability at the ecosystem scale. Infection-enhanced fluxes of dissolved inorganic nitrogen were similar to other commonly important environmental sources of bioavailable nitrogen to the system. Additional field measurements within nitrogen-limited ponds indicated that nitrogen flux rates from the periphyton to the water column in high-snail density/high-infection ponds were up to 50% higher than low-infection ponds. By altering host nutrient assimilation/excretion flexibility, parasites could play a widespread, but currently unrecognized, role in ecosystem nutrient cycling, especially when parasite and host abundances are high and hosts play a central role in ecosystem nutrient cycling. © 2016 The Authors. Journal of Animal Ecology © 2016 British Ecological Society.

Impaired hypothalamic mTOR activation in the adult rat offspring born to mothers fed a low-protein diet.

PubMed

Guzmán-Quevedo, Omar; Da Silva Aragão, Raquel; Pérez García, Georgina; Matos, Rhowena J B; de Sa Braga Oliveira, André; Manhães de Castro, Raul; Bolaños-Jiménez, Francisco

2013-01-01

Several epidemiological and experimental studies have clearly established that maternal malnutrition induces a high risk of developing obesity and related metabolic diseases in the offspring. To determine if altered nutrient sensing might underlie this enhanced disease susceptibility, here we examined the effects of perinatal protein restriction on the activation of the nutrient sensor mTOR in response to acute variations in the nutritional status of the organism. Female Wistar rats were fed isocaloric diets containing either 17% protein (control) or 8% protein (PR) throughout pregnancy and lactation. At weaning offspring received standard chow and at 4 months of age the effects of fasting or fasting plus re-feeding on the phosphorylation levels of mTOR and its downstream target S6 ribosomal protein (rpS6) in the hypothalamus were assessed by immuno-fluorescence and western blot. Under ad libitum feeding conditions, PR rats exhibited decreased mTOR and rpS6 phosphorylation in the arcuate (ARC) and ventromedial (VMH) hypothalamic nuclei. Moreover, the phosphorylation of mTOR and rpS6 in these hypothalamic nuclei decreased with fasting in control but not in PR animals. Conversely, PR animals exhibited enhanced number of pmTOR imunostained cells in the paraventricular nucleus (PVN) and fasting decreased the activation of mTOR in the PVN of malnourished but not of control rats. These alterations occurred at a developmental stage at which perinatally-undernourished animals do not show yet obesity or glucose intolerance. Collectively, our observations suggest that altered hypothalamic nutrient sensing in response to an inadequate foetal and neonatal energetic environment is one of the basic mechanisms of the developmental programming of metabolic disorders and might play a causing role in the development of the metabolic syndrome induced by malnutrition during early life.

The Regulatory Networks That Control Clostridium difficile Toxin Synthesis

PubMed Central

Martin-Verstraete, Isabelle; Peltier, Johann; Dupuy, Bruno

2016-01-01

The pathogenic clostridia cause many human and animal diseases, which typically arise as a consequence of the production of potent exotoxins. Among the enterotoxic clostridia, Clostridium difficile is the main causative agent of nosocomial intestinal infections in adults with a compromised gut microbiota caused by antibiotic treatment. The symptoms of C. difficile infection are essentially caused by the production of two exotoxins: TcdA and TcdB. Moreover, for severe forms of disease, the spectrum of diseases caused by C. difficile has also been correlated to the levels of toxins that are produced during host infection. This observation strengthened the idea that the regulation of toxin synthesis is an important part of C. difficile pathogenesis. This review summarizes our current knowledge about the regulators and sigma factors that have been reported to control toxin gene expression in response to several environmental signals and stresses, including the availability of certain carbon sources and amino acids, or to signaling molecules, such as the autoinducing peptides of quorum sensing systems. The overlapping regulation of key metabolic pathways and toxin synthesis strongly suggests that toxin production is a complex response that is triggered by bacteria in response to particular states of nutrient availability during infection. PMID:27187475

Short-term fate of intertidal microphytobenthos carbon under enhanced nutrient availability: a 13C pulse-chase experiment

NASA Astrophysics Data System (ADS)

Riekenberg, Philip M.; Oakes, Joanne M.; Eyre, Bradley D.

2018-05-01

Shallow coastal waters in many regions are subject to nutrient enrichment. Microphytobenthos (MPB) can account for much of the carbon (C) fixation in these environments, depending on the depth of the water column, but the effect of enhanced nutrient availability on the processing and fate of MPB-derived C (MPB-C) is relatively unknown. In this study, MPB was labeled (stable isotope enrichment) in situ using 13C-sodium bicarbonate. The processing and fate of the newly fixed MPB-C was then traced using ex situ incubations over 3.5 days under different concentrations of nutrients (NH4+ and PO43-: ambient, 2 × ambient, 5 × ambient, and 10 × ambient). After 3.5 days, sediments incubated with increased nutrient concentrations (amended treatments) had increased loss of 13C from sediment organic matter (OM) as a portion of initial uptake (95 % remaining in ambient vs. 79-93 % for amended treatments) and less 13C in MPB (52 % ambient, 26-49 % amended), most likely reflecting increased turnover of MPB-derived C supporting increased production of extracellular enzymes and storage products. Loss of MPB-derived C to the water column via dissolved organic C (DOC) was minimal regardless of treatment (0.4-0.6 %). Loss due to respiration was more substantial, with effluxes of dissolved inorganic C (DIC) increasing with additional nutrient availability (4 % ambient, 6.6-19.8 % amended). These shifts resulted in a decreased turnover time for algal C (419 days ambient, 134-199 days amended). This suggests that nutrient enrichment of estuaries may ultimately lead to decreased retention of carbon within MPB-dominated sediments.

Increased plant growth from nitrogen addition should conserve phosphorus in terrestrial ecosystems.

PubMed

Perring, Michael P; Hedin, Lars O; Levin, Simon A; McGroddy, Megan; de Mazancourt, Claire

2008-02-12

Inputs of available nitrogen (N) to ecosystems have grown over the recent past. There is limited general understanding of how increased N inputs affect the cycling and retention of other potentially limiting nutrients. Using a plant-soil nutrient model, and by explicitly coupling N and phosphorus (P) in plant biomass, we examine the impact of increasing N supply on the ecosystem cycling and retention of P, assuming that the main impact of N is to increase plant growth. We find divergent responses in the P cycle depending on the specific pathway by which nutrients are lost from the ecosystem. Retention of P is promoted if the relative propensity for loss of plant available P is greater than that for the loss of less readily available organic P. This is the first theoretical demonstration that the coupled response of ecosystem-scale nutrient cycles critically depends on the form of nutrient loss. P retention might be lessened, or reversed, depending on the kinetics and size of a buffering reactive P pool. These properties determine the reactive pool's ability to supply available P. Parameterization of the model across a range of forest ecosystems spanning various environmental and climatic conditions indicates that enhanced plant growth due to increased N should trigger increased P conservation within ecosystems while leading to more dissolved organic P loss. We discuss how the magnitude and direction of the effect of N may also depend on other processes.

Geophysical evidence for non-uniform permafrost degradation after fire across boreal landscapes

NASA Astrophysics Data System (ADS)

Minsley, B. J.; Pastick, N. J.; Wylie, B. K.; Brown, D. N.; Kass, A.

2015-12-01

Fire can be a significant driver of permafrost change in boreal landscapes, altering the availability of soil carbon and nutrients that have important implications for future climate and ecological succession. However, not all landscapes are equally susceptible to fire-induced change. As fire frequency is expected to increase in the high latitudes, methods to understand the vulnerability and resilience of different landscapes to permafrost degradation are needed. We present a combination of multi-scale remote sensing, geophysical, and field observations that reveal details of both near-surface (<1 m) and deeper impacts of fire on permafrost. Along 11 transects that span burned-unburned boundaries in different landscape settings within interior Alaska, subsurface imaging indicates locations where permafrost appears to be resilient to disturbance from fire, areas where warm permafrost conditions exist that may be most vulnerable to future change, and also where permafrost has thawed. High-resolution geophysical data corroborate remote sensing interpretations of near-surface permafrost, and also add new high-fidelity details of spatial heterogeneity that extend from the shallow subsurface to depths of about 10 m. Data collected along each transect include observations of active layer thickness (ALT), organic layer thickness (OLT), plant species cover, electrical resistivity tomography (ERT), and downhole Nuclear Magnetic Resonance (NMR) measurements. Results show that post-fire impacts on permafrost can be variable, and depend on multiple factors such as fire severity, soil texture, and soil moisture.

Polar transport in plants mediated by membrane transporters: focus on mechanisms of polar auxin transport.

PubMed

Naramoto, Satoshi

2017-12-01

Directional cell-to-cell transport of functional molecules, called polar transport, enables plants to sense and respond to developmental and environmental signals. Transporters that localize to plasma membranes (PMs) in a polar manner are key components of these systems. PIN-FORMED (PIN) auxin efflux carriers, which are the most studied polar-localized PM proteins, are implicated in the polar transport of auxin that in turn regulates plant development and tropic growth. In this review, the regulatory mechanisms underlying polar localization of PINs, control of auxin efflux activity, and PIN abundance at PMs are considered. Up to date information on polar-localized nutrient transporters that regulate directional nutrient movement from soil into the root vasculature is also discussed. Copyright © 2017 Elsevier Ltd. All rights reserved.

The effects of CO2 and nutrient fertilisation on the growth and temperature response of the mangrove Avicennia germinans.

PubMed

Reef, Ruth; Slot, Martijn; Motro, Uzi; Motro, Michal; Motro, Yoav; Adame, Maria F; Garcia, Milton; Aranda, Jorge; Lovelock, Catherine E; Winter, Klaus

2016-08-01

In order to understand plant responses to both the widespread phenomenon of increased nutrient inputs to coastal zones and the concurrent rise in atmospheric CO2 concentrations, CO2-nutrient interactions need to be considered. In addition to its potential stimulating effect on photosynthesis and growth, elevated CO2 affects the temperature response of photosynthesis. The scarcity of experiments testing how elevated CO2 affects the temperature response of tropical trees hinders our ability to model future primary productivity. In a glasshouse study, we examined the effects of elevated CO2 (800 ppm) and nutrient availability on seedlings of the widespread mangrove Avicennia germinans. We assessed photosynthetic performance, the temperature response of photosynthesis, seedling growth and biomass allocation. We found large synergistic gains in both growth (42 %) and photosynthesis (115 %) when seedlings grown under elevated CO2 were supplied with elevated nutrient concentrations relative to their ambient growing conditions. Growth was significantly enhanced under elevated CO2 only under high-nutrient conditions, mainly in above-ground tissues. Under low-nutrient conditions and elevated CO2, root volume was more than double that of seedlings grown under ambient CO2 levels. Elevated CO2 significantly increased the temperature optimum for photosynthesis by ca. 4 °C. Rising CO2 concentrations are likely to have a significant positive effect on the growth rate of A. germinans over the next century, especially in areas where nutrient availability is high.

Aggregating land use quantity and intensity to link water quality in upper catchment of Miyun Reservoir

NASA Astrophysics Data System (ADS)

Xu, E.

2015-12-01

Land use is closely related to hydrological and biochemical processes influencing the water quality. Quantifying relationship between both of them can help effectively manage land use to improve water quality. Previous studies majorly utilized land use quantity as an indicator to link water quality parameters, which lacked an insight to the influence of land use intensity. Taking upper catchment of Miyun Reservoir as a case study, we proposed a method of aggregating land use quantity and intensity to build a new land use indicator and investigated its explanation empower on water quality. Six nutrient concentrations from 52 sub-watersheds covering the whole catchment were used to characterize spatial distributions of water eutrophication. Based on spatial techniques and empirical conversion coefficients, combined remote sensing with socio-economic statistical data, land use intensity was measured and mapped visually. Then the new land use indicator was calculated and linked to nutrient concentrations by Pearson correlation coefficients. Results demonstrated that our new land use indicator incorporating intensity information can quantify the potential different nutrients exporting abilities from land uses. Comparing to traditional indicators only characterized by land use quantity, most Pearson correlation coefficients between new indicator and water nutrient concentrations increased. New information enhanced the explanatory power of land use on water nutrient concentrations. Then it can help better understand the impact of land use on water quality and guide land use management for supporting decision making.

Legacy Phosphorus Effect and Need to Re-calibrate Soil Test P Methods for Organic Crop Production.

NASA Astrophysics Data System (ADS)

Dao, Thanh H.; Schomberg, Harry H.; Cavigelli, Michel A.

2015-04-01

Phosphorus (P) is a required nutrient for the normal development and growth of plants and supplemental P is needed in most cultivated soils. Large inputs of cover crop residues and nutrient-rich animal manure are added to supply needed nutrients to promote the optimal production of organic grain crops and forages. The effects of crop rotations and tillage management of the near-surface zone on labile phosphorus (P) forms were studied in soil under conventional and organic crop management systems in the mid-Atlantic region of the U.S. after 18 years due to the increased interest in these alternative systems. Soil nutrient surpluses likely caused by low grain yields resulted in large pools of exchangeable phosphate-P and equally large pools of enzyme-labile organic P (Po) in soils under organic management. In addition, the difference in the P loading rates between the conventional and organic treatments as guided by routine soil test recommendations suggested that overestimating plant P requirements contributed to soil P surpluses because routine soil testing procedures did not account for the presence and size of the soil enzyme-labile Po pool. The effect of large P additions is long-lasting as they continued to contribute to elevated soil total bioactive P concentrations 12 or more years later. Consequently, accurate estimates of crop P requirements, P turnover in soil, and real-time plant and soil sensing systems are critical considerations to optimally manage manure-derived nutrients in organic crop production.

Relative Roles of Soil Moisture, Nutrient Supply, Depth, and Mechanical Impedance in Determining Composition and Structure of Wisconsin Prairies

PubMed Central

Wernerehl, Robert W.; Givnish, Thomas J.

2015-01-01

Ecologists have long classified Midwestern prairies based on compositional variation assumed to reflect local gradients in moisture availability. The best known classification is based on Curtis’ continuum index (CI), calculated using the presence of indicator species thought centered on different portions of an underlying moisture gradient. Direct evidence of the extent to which CI reflects differences in moisture availability has been lacking, however. Many factors that increase moisture availability (e.g., soil depth, silt content) also increase nutrient supply and decrease soil mechanical impedance; the ecological effects of the last have rarely been considered in any ecosystem. Decreased soil mechanical impedance should increase the availability of soil moisture and nutrients by reducing the root costs of retrieving both. Here we assess the relative importance of soil moisture, nutrient supply, and mechanical impedance in determining prairie composition and structure. We used leaf δ13C of C3 plants as a measure of growing-season moisture availability, cation exchange capacity (CEC) x soil depth as a measure of mineral nutrient availability, and penetrometer data as a measure of soil mechanical impedance. Community composition and structure were assessed in 17 remnant prairies in Wisconsin which vary little in annual precipitation. Ordination and regression analyses showed that δ13C increased with CI toward “drier” sites, and decreased with soil depth and % silt content. Variation in δ13C among remnants was 2.0‰, comparable to that along continental gradients from ca. 500–1500 mm annual rainfall. As predicted, LAI and average leaf height increased significantly toward “wetter” sites. CI accounted for 54% of compositional variance but δ13C accounted for only 6.2%, despite the strong relationships of δ13C to CI and CI to composition. Compositional variation reflects soil fertility and mechanical impedance more than moisture availability. This study is the first to quantify the effects of soil mechanical impedance on community ecology. PMID:26368936

Relative Roles of Soil Moisture, Nutrient Supply, Depth, and Mechanical Impedance in Determining Composition and Structure of Wisconsin Prairies.

PubMed

Wernerehl, Robert W; Givnish, Thomas J

2015-01-01

Ecologists have long classified Midwestern prairies based on compositional variation assumed to reflect local gradients in moisture availability. The best known classification is based on Curtis' continuum index (CI), calculated using the presence of indicator species thought centered on different portions of an underlying moisture gradient. Direct evidence of the extent to which CI reflects differences in moisture availability has been lacking, however. Many factors that increase moisture availability (e.g., soil depth, silt content) also increase nutrient supply and decrease soil mechanical impedance; the ecological effects of the last have rarely been considered in any ecosystem. Decreased soil mechanical impedance should increase the availability of soil moisture and nutrients by reducing the root costs of retrieving both. Here we assess the relative importance of soil moisture, nutrient supply, and mechanical impedance in determining prairie composition and structure. We used leaf δ13C of C3 plants as a measure of growing-season moisture availability, cation exchange capacity (CEC) x soil depth as a measure of mineral nutrient availability, and penetrometer data as a measure of soil mechanical impedance. Community composition and structure were assessed in 17 remnant prairies in Wisconsin which vary little in annual precipitation. Ordination and regression analyses showed that δ13C increased with CI toward "drier" sites, and decreased with soil depth and % silt content. Variation in δ13C among remnants was 2.0‰, comparable to that along continental gradients from ca. 500-1500 mm annual rainfall. As predicted, LAI and average leaf height increased significantly toward "wetter" sites. CI accounted for 54% of compositional variance but δ13C accounted for only 6.2%, despite the strong relationships of δ13C to CI and CI to composition. Compositional variation reflects soil fertility and mechanical impedance more than moisture availability. This study is the first to quantify the effects of soil mechanical impedance on community ecology.

Influence of Channel Geomorphology on Retention of Dissolved and Particulate Matter in a Cascade Mountain Stream

Treesearch

Gary A. Lamberti; Stan V. Gregory; Linda R. Ashkenas; Randall C. Wildman; Alan G. Steinman

1989-01-01

Retention of particulate and dissolved nutrients in streams is a major determinant of food avail-ability to stream biota. Retention of particulate matter (leaves) and dissolved nutrients (nitrogen) was studied experimentally during summer 1987 in four 300-500 m reaches of Lookout Creek, a fifth-order stream in the Cascade Mountains of Oregon. Constrained (narrow valley...

SPRUCE Plant-Available Nutrients Assessed with Ion-Exchange Resins in Experimental Plots, Beginning in 2013

DOE Data Explorer

Iversen, C. M. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A; Latimer, J. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A; Burnham, A. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A; Brice, D. J. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A; Childs, J. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A; Vander Stel, H. M. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A

2017-01-01

This data set consists of observations of plant-available nutrients assessed using ion-exchange resin capsules incubated serially in aerobic and anaerobic peat layers beginning in 2013 in the SPRUCE experimental plots at four depths in hummock microtopography and two depths in hollow microtopography. Data will be periodically added to until the conclusion of the SPRUCE experiment.

Radiation-use efficiency and gas exchange responses to water and nutrient availability in irrigated and fertilized stands of sweetgum and sycamore

Treesearch

Christopher B. Allen; Rodney E. Will; Robert C. McGravey; David R. Coyle; Mark D. Coleman

2005-01-01

We investigated how water and nutrient availability affect radiation-use effeciency (e) and assessed leaf gas exchange as a possible mechanism for shifts in e. We measured aboveground net primary production (ANPP) and annual photosynthetically active radiation (PAR) capture to calculate e as well as leaf-level physiological variables (light-saturated net photosynthesis...

Evaluation of commercially available enzymes, probiotics, or yeast on apparent total-tract nutrient digestion and growth in nursery and finishing pigs fed diets containing corn dried distillers grains with solubles

USDA-ARS?s Scientific Manuscript database

The ability of enzymes, direct fed microbials, or yeast to enhance nutrient utilization or growth performance in nursery or finishing pigs fed diets containing increased levels of corn fiber from dried distillers grains with solubles (DDGS) is largely unknown. Ten commercially available feed additiv...

Complementary feeding recommendations based on locally available foods in Indonesia.

PubMed

Fahmida, Umi; Santika, Otte; Kolopaking, Risatianti; Ferguson, Elaine

2014-12-01

Affordable, locally contextual complementary feeding recommendations (CFRs) that take into account cultural diversity and differences in food availability will be more likely to result in long-term improvements in complementary feeding practices than general recommendations. More objective approaches, such as linear programming (LP), have been recommended to identify optimal but CFRs to meet nutrient requirements given local food availability, food patterns, food portions, and cost. To present results of our previous studies in which we developed CFRs using LP and to provide an example of how these CFRs can be put into practice in a community intervention trial in Indonesia. Dietary data were obtained using single 24-hour dietary recall or 1-day weighed diet record combined with 1-day 24-hour recall and 5-day food intake tally. With the use of the LP approach, nutrient intakes were optimized while ensuring that a realistic diet was selected by using constraints such as the diet's energy content, food patterns, food portions, and cost. The price per 100 g of edible portion was obtained from market surveys in two or three local markets in each study area. LP analysis was performed using Super Solver in MS Excel or Optifood software. Iron, zinc, calcium, and niacin were problem nutrients in all age groups of children (6 to 8, 9 to 11, and 12 to 23 months) in both rural and periurban areas, except among children of higher socioeconomic status in urban areas. Thiamin and folate were also problem nutrients found in some settings. Animal-source foods (meat, fish, poultry, and eggs [MFPE] and fortified foods were the nutrient-dense foods identified by LP to fill the nutrient gaps of these problem nutrients. Iron, calcium, zinc, niacin, and potentially folate and thiamine are typical "problem nutrients" in complementary foods of Indonesian children. However, the extent of dietary inadequacy varies across age groups, area, and socioeconomic level. MFPE and fortified foods can improve micronutrient adequacy in complementary feeding diets and should be promoted in CFRs.

Practice Paper of the Academy of Nutrition and Dietetics: Selecting Nutrient-Dense Foods for Good Health.

PubMed

Hingle, Melanie D; Kandiah, Jayanthi; Maggi, Annette

2016-09-01

The 2015 Dietary Guidelines for Americans encourage selection of nutrient-dense foods for health promotion and disease prevention and management. The purpose of this Academy of Nutrition and Dietetics practice paper is to provide an update regarding the science and practice of nutrient-dense food identification and selection. Characterization of tools used to identify nutrient density of foods is provided and recommendations for how registered dietitian nutritionists and nutrition and dietetics technicians, registered, might use available profiling tools to help consumers select nutrient-dense foods is discussed. Copyright © 2016 Academy of Nutrition and Dietetics. Published by Elsevier Inc. All rights reserved.

Characterization of Alcohol-induced Filamentous Growth in Saccharomyces cerevisiae

PubMed Central

Lorenz, Michael C.; Cutler, N. Shane; Heitman, Joseph

2000-01-01

Diploid cells of the budding yeast Saccharomyces cerevisiae starved for nitrogen differentiate into a filamentous growth form. Poor carbon sources such as starches can also stimulate filamentation, whereas haploid cells undergo a similar invasive growth response in rich medium. Previous work has demonstrated a role for various alcohols, by-products of amino acid metabolism, in altering cellular morphology. We found that several alcohols, notably isoamyl alcohol and 1-butanol, stimulate filamentous growth in haploid cells in which this differentiation is normally repressed. Butanol also induces cell elongation and changes in budding pattern, leading to a pseudohyphal morphology, even in liquid medium. The filamentous colony morphology and cell elongation require elements of the pheromone-responsive MAPK cascade and TEC1, whereas components of the nutrient-sensing machinery, such as MEP2, GPA2, and GPR1, do not affect this phenomenon. A screen for 1-butanol–insensitive mutants identified additional proteins that regulate polarized growth (BUD8, BEM1, BEM4, and FIG1), mitochondrial function (MSM1, MRP21, and HMI1), and a transcriptional regulator (CHD1). Furthermore, we have also found that ethanol stimulates hyperfilamentation in diploid cells, again in a MAPK-dependent manner. Together, these results suggest that yeast may sense a combination of nutrient limitation and metabolic by-products to regulate differentiation. PMID:10637301

Nutrient sensing by the mitochondrial transcription machinery dictates oxidative phosphorylation

PubMed Central

Liu, Lijun; Nam, Minwoo; Fan, Wei; Akie, Thomas E.; Hoaglin, David C.; Gao, Guangping; Keaney, John F.; Cooper, Marcus P.

2014-01-01

Sirtuin 3 (SIRT3), an important regulator of energy metabolism and lipid oxidation, is induced in fasted liver mitochondria and implicated in metabolic syndrome. In fasted liver, SIRT3-mediated increases in substrate flux depend on oxidative phosphorylation (OXPHOS), but precisely how OXPHOS meets the challenge of increased substrate oxidation in fasted liver remains unclear. Here, we show that liver mitochondria in fasting mice adapt to the demand of increased substrate oxidation by increasing their OXPHOS efficiency. In response to cAMP signaling, SIRT3 deacetylated and activated leucine-rich protein 130 (LRP130; official symbol, LRPPRC), promoting a mitochondrial transcriptional program that enhanced hepatic OXPHOS. Using mass spectrometry, we identified SIRT3-regulated lysine residues in LRP130 that generated a lysine-to-arginine (KR) mutant of LRP130 that mimics deacetylated protein. Compared with wild-type LRP130 protein, expression of the KR mutant increased mitochondrial transcription and OXPHOS in vitro. Indeed, even when SIRT3 activity was abolished, activation of mitochondrial transcription and OXPHOS by the KR mutant remained robust, further highlighting the contribution of LRP130 deacetylation to increased OXPHOS in fasted liver. These data establish a link between nutrient sensing and mitochondrial transcription that regulates OXPHOS in fasted liver and may explain how fasted liver adapts to increased substrate oxidation. PMID:24430182

Metabolism meets immunity: The role of free fatty acid receptors in the immune system.

PubMed

Alvarez-Curto, Elisa; Milligan, Graeme

2016-08-15

There are significant numbers of nutrient sensing G protein-coupled receptors (GPCRs) that can be found in cells of the immune system and in tissues that are involved in metabolic function, such as the pancreas or the intestinal epithelium. The family of free fatty acid receptors (FFAR1-4, GPR84), plus a few other metabolite sensing receptors (GPR109A, GPR91, GPR35) have been for this reason the focus of studies linking the effects of nutrients with immunological responses. A number of the beneficial anti-inflammatory effects credited to dietary fats such as omega-3 fatty acids are attributed to their actions on FFAR4.This might play an important protective role in the development of obesity, insulin resistance or asthma. The role of the short-chain fatty acids resulting from fermentation of fibre by the intestinal microbiota in regulating acute inflammatory responses is also discussed. Finally we assess the therapeutic potential of this family of receptors to treat pathologies where inflammation is a major factor such as type 2 diabetes, whether by the use of novel synthetic molecules or by the modulation of the individual's diet. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Spatial and phylogenetic variation in plant defense in a tropical moist forest canopy community

NASA Astrophysics Data System (ADS)

McManus, K. M.; Asner, G. P.; Martin, R.

2013-12-01

Plants employ physical and chemical defenses to mitigate damage caused by herbivory. Spatial patterns of plant defense may provide insight into the role of plant-herbivore interactions in the assembly of plant communities. Within plant communities, the spatial overdispersion of anti-herbivore defenses by individuals may reflect a strategy to avoid host shifts from herbivore assemblages of neighboring plants. However, variation in plant defense may also result from trade-offs between foliar investment into defense and growth, mediated by variations in abiotic nutrient availability, or constrained by phylogeny. We measured four defensive traits (leaf toughness, total phenols, condensed tannins, and hydrolysable tannins) and three growth traits (LMA, C:N, total protein) of outer canopy foliage for 345 canopy trees representing 78 species, 65 genera, and 34 families in a moist tropical rainforest on Barro Colorado Island, Panama. The outer canopy provides an important, but rarely evaluated, cross-sectional image of the tropical forest ecosystem, and observations at this scale may provide an important link between field and remote sensing based studies. We used existing data on edaphic and geological properties to investigate the relationships of abiotic nutrient variation on variation in defense. Using regression and nested random-effects variance modeling, we found strong phylogenetic association with defensive traits at the family and species level, and little evidence for a trade-off between defensive traits. Greater understanding of phylogenetic structure in trait variation may yield improved characterizations of tropical biodiversity, from functional traits to risk assessments.

Terrestrial biogeochemical cycles: global interactions with the atmosphere and hydrology

NASA Astrophysics Data System (ADS)

Schimel, David S.; Kittel, Timothy G. F.; Parton, William J.

1991-08-01

Ecosystem scientists have developed a body of theory to predict the behaviour of biogeochemical cycles when exchanges with other ecosystems are small or prescribed. Recent environmental changes make it clear that linkages between ecosystems via atmospheric and hydrological transport have large effects on ecosystem dynamics when considered over time periods of a decade to a century, time scales relevant to contemporary humankind. Our ability to predict behaviour of ecosystems coupled by transport is limited by our ability (1) to extrapolate biotic function to large spatial scales and (2) to measure and model transport. We review developments in ecosystem theory, remote sensing, and geographical information systems (GIS) that support new efforts in spatial modeling. A paradigm has emerged to predict behaviour of ecosystems based on understanding responses to multiple resources (e.g., water, nutrients, light). Several ecosystem models couple primary production to decomposition and nutrient availability using the above paradigm. These models require a fairly small set of environmental variables to simulate spatial and temporal variation in rates of biogeochemical cycling. Simultaneously, techniques for inferring ecosystem behaviour from remotely measured canopy light interception are improving our ability to infer plant activity from satellite observations. Efforts have begun to couple models of transport in air and water to models of ecosystem function. Preliminary work indicates that coupling of transport and ecosystem processes alters the behaviour of earth system components (hydrology, terrestrial ecosystems, and the atmosphere) from that of an uncoupled mode.

Factors affecting the growth of Didymosphenia geminata in New Zealand rivers: Flow, bed disturbance, nutrients, light, and seasonal dynamics. (Invited)

NASA Astrophysics Data System (ADS)

Cullis, J. D.; Gillis, C.; Drummond, J. D.; Garcia, T.; Kilroy, C.; Larned, S.; Hassan, M. A.

2010-12-01

Didymosphenia geminata (didymo) was introduced into a New Zealand river in 2004, and since then has dramatically spread to cover the beds of many rivers with extremely dense and extensive mats. Successful management is hampered by the fact that much is still unknown about the factors affecting the growth of this nuisance species. We synthesized available data on the distribution of D. geminata in New Zealand rivers to determine how physical and chemical system conditions (flow, bed disturbance, nutrients, and light) affect the growth and persistence of this organism. Here we assess results from bi-weekly surveys performed over a full year on two rivers where didymo was first observed in New Zealand; the Oreti and Mararoa. We used the data to test the hypotheses that the development of thick, dense mats requires high light levels but is inversely proportional to nutrient levels, and that mat persistence is controlled by the frequency of flow events that produce bed sediment transport. Observed regrowth between disturbance events was found to be inversely correlated with nutrient availability. The seasonal availability of light did not correlate with variations in growth rate, but this did not account for specific characteristics of the different sites such as aspect, shading, flow depth and turbidity that will all impact on the amount of available light reaching the streambed. The results clearly indicate that the time-history of flow and nutrient levels is critical to evaluating the growth and persistence of D. geminata and that additional site specific information is necessary to determine the role of bed stability and the amount of available light reaching the streambed.

Aeolian dust nutrient contributions increase with substrate age in semi-arid ecosystems

NASA Astrophysics Data System (ADS)

Coble, A. A.; Hart, S. C.; Ketterer, M. E.; Newman, G. S.

2013-12-01

Rock-derived nutrients supplied by mineral weathering become depleted over time, and without an additional nutrient source the ecosystem may eventually regress or reach a terminal steady state. Previous studies have demonstrated that aeolian dust act as parent materials of soils and important nutrients to plants in arid regions, but the relative importance of these exogenous nutrients to the function of dry ecosystems during soil development is uncertain. Here, using strontium isotopes as a tracer and a well-constrained, three million year old substrate age gradient, we show that aeolian-derived nutrients become increasingly important to plant-available soil pools and tree (Pinus edulis) growth during the latter stages of soil development in a semi-arid climate. Furthermore, the depth of nutrient uptake increased on older substrates, suggesting that trees in arid regions acquire nutrients from greater depths as ecosystem development progresses presumably in response to nutrient depletion in the more weathered surface soils. Our results contribute to the unification of biogeochemical theory by demonstrating the similarity in roles of atmospheric nutrient inputs during ecosystem development across contrasting climates.

Hyperspectral imaging of water quality - past applications and future directions.

NASA Astrophysics Data System (ADS)

Ross, M. R. V.; Pavelsky, T.

2017-12-01

Inland waters control the delivery of sediment, carbon, and nutrients from land to ocean by transforming, depositing, and transporting constituents downstream. However, the dominant in situ conditions that control these processes are poorly constrained, especially at larger spatial scales. Hyperspectral imaging, a remote sensing technique that uses reflectance in hundreds of narrow spectral bands, can be used to estimate water quality parameters like sediment and carbon concentration over larger water bodies. Here, we review methods and applications for using hyperspectral imagery to generate near-surface two-dimensional models of water quality in lakes and rivers. Further, we show applications using newly available data from the National Ecological Observation Network aerial observation platform in the Black Warrior and Tombigbee Rivers, Alabama. We demonstrate large spatial variation in chlorophyll, colored dissolved organic matter, and turbidity in each river and uneven mixing of water quality constituents for several kilometers. Finally, we demonstrate some novel techniques using hyperspectral imagery to deconvolve dissolved organic matter spectral signatures to specific organic matter components.

The availability of conventional forms of remotely sensed data

USGS Publications Warehouse

Sturdevant, James A.; Holm, Thomas M.

1982-01-01

For decades Federal and State agencies have been collecting aerial photographs of various film types and scales over parts of the United States. More recently, worldwide Earth resources data acquired by orbiting satellites have inundated the remote sensing community. Determining the types of remotely sensed data that are publicly available can be confusing to the land-resource manager, planner, and scientist. This paper is a summary of the more commonly used types of remotely sensed data (aircraft and satellite) and their public availability. Special emphasis is placed on the National High-Altitude Photography (NHAP) program and future remote-sensing satellites.

Context-dependency in the effects of nutrient loading and consumers on the availability of space in marine rocky environments.

PubMed

Bulleri, Fabio; Russell, Bayden D; Connell, Sean D

2012-01-01

Enhanced nutrient loading and depletion of consumer populations interact to alter the structure of aquatic plant communities. Nonetheless, variation between adjacent habitats in the relative strength of bottom-up (i.e. nutrients) versus top-down (i.e. grazing) forces as determinants of community structure across broad spatial scales remains unexplored. We experimentally assessed the importance of grazing pressure and nutrient availability on the development of macroalgal assemblages and the maintenance of unoccupied space in habitats differing in physical conditions (i.e. intertidal versus subtidal), across regions of contrasting productivity (oligotrophic coasts of South Australia versus the more productive coasts of Eastern Australia). In Eastern Australia, grazers were effective in maintaining space free of macroalgae in both intertidal and subtidal habitats, irrespective of nutrient levels. Conversely, in South Australia, grazers could not prevent colonization of space by turf-forming macroalgae in subtidal habitats regardless of nutrients levels, yet in intertidal habitats removal of grazers reduced unoccupied space when nutrients were elevated. Assessing the effects of eutrophication in coastal waters requires balancing our understanding between local consumer pressure and background oceanographic conditions that affect productivity. This broader-based understanding may assist in reconciling disproportionately large local-scale variation, a characteristic of ecology, with regional scale processes that are often of greater relevance to policy making and tractability to management.

Effects of Soil Texture on Belowground Carbon and Nutrient Storage in a Lowland Amazonian Forest Ecosystem.

Treesearch

Whendee L. Silver; Jason Neff; Megan McGroddy; Ed Veldkamp; Michael Keller; Raimundo Cosme

2000-01-01

Soil texture plays a key role in belowground C storage in forest ecosystems and strongly influences nutrient availability and retention, particularly in highly weathered soils. We used field data and the Century ecosystem model to explore the role of soil texture in belowground C storage, nutrient pool sizes, and N fluxes in highly weathered soils in an Amazonian...

Diel plant water use and competitive soil cation exchange interact to enhance NH 4 + and K + availability in the rhizosphere

DOE PAGES

Espeleta, Javier F.; Cardon, Zoe G.; Mayer, K. Ulrich; ...

2016-11-12

Hydro-biogeochemical processes in the rhizosphere regulate nutrient and water availability, and thus ecosystem productivity. We hypothesized that two such processes often neglected in rhizosphere models — diel plant water use and competitive cation exchange — could interact to enhance availability of K + and NH 4 +, both high-demand nutrients. A rhizosphere model with competitive cation exchange was used to investigate how diel plant water use (i.e., daytime transpiration coupled with no nighttime water use, with nighttime root water release, and with nighttime transpiration) affects competitive ion interactions and availability of K + and NH 4 +. Competitive cation exchangemore » enabled lowdemand cations that accumulate against roots (Ca 2+, Mg 2+, Na +) to desorb NH 4 + and K + from soil, generating non-monotonic dissolved concentration profiles (i.e. ‘hotspots’ 0.1–1 cm from the root). Cation accumulation and competitive desorption increased with net root water uptake. Daytime transpiration rate controlled diel variation in NH 4 + and K + aqueous mass, nighttime water use controlled spatial locations of ‘hotspots’, and day-to-night differences in water use controlled diel differences in ‘hotspot’ concentrations. Finally, diel plant water use and competitive cation exchange enhanced NH 4 + and K + availability and influenced rhizosphere concentration dynamics. Demonstrated responses have implications for understanding rhizosphere nutrient cycling and plant nutrient uptake.« less

Nitrogen to phosphorus ratio of plant biomass versus soil solution in a tropical pioneer tree, Ficus insipida.

PubMed

Garrish, Valerie; Cernusak, Lucas A; Winter, Klaus; Turner, Benjamin L

2010-08-01

It is commonly assumed that the nitrogen to phosphorus (N:P) ratio of a terrestrial plant reflects the relative availability of N and P in the soil in which the plant grows. Here, this was assessed for a tropical pioneer tree, Ficus insipida. Seedlings were grown in sand and irrigated with nutrient solutions containing N:P ratios ranging from <1 to >100. The experimental design further allowed investigation of physiological responses to N and P availability. Homeostatic control over N:P ratios was stronger in leaves than in stems or roots, suggesting that N:P ratios of stems and roots are more sensitive indicators of the relative availability of N and P at a site than N:P ratios of leaves. The leaf N:P ratio at which the largest plant dry mass and highest photosynthetic rates were achieved was approximately 11, whereas the corresponding whole-plant N:P ratio was approximately 6. Plant P concentration varied as a function of transpiration rate at constant nutrient solution P concentration, possibly due to transpiration-induced variation in the mass flow of P to root surfaces. The transpiration rate varied in response to nutrient solution N concentration, but not to nutrient solution P concentration, demonstrating nutritional control over transpiration by N but not P. Water-use efficiency varied as a function of N availability, but not as a function of P availability.

[Study on nutrient and salinity in soil covered with different vegetations in Shuangtaizi estuarine wetlands].

PubMed

Song, Xiao-Lin; Lü, Xian-Guo; Zhang, Zhong-Sheng; Chen, Zhi-Ke; Liu, Zheng-Mao

2011-09-01

Nutrient elements and salinity in soil covered by different vegetations including Phragmites australis (Clay.) Trin., Typha orientalis Presl., Puccinellia distans Parl, and Suaeda salsa in Shuangtaizi estuarine wetlands were investigated to study their distribution characteristics and to reveal the nutrient element variation during the vegetation succession processes. Results indicated that total potassium, total phosphorus and salinity were different significantly in soil between different plant communities while available phosphorus, total nitrogen, available nitrogen, available potassium, total sulfur, iron and soil organic carbon were different insignificantly. Correlation analysis suggested that soil organic carbon were related significantly to total nitrogen, available phosphorus, available potassium, which implied that decomposition of plant litter might be the mail source of soil nitrogen and available nutrient. Salinity was significantly related to total phosphorus and iron in soil. In Shuangtaizi estuarine wetland soil, ratios of carbon to nitrogen (R(C/N)) was in the range of 12.21-26.33 and the average value was 18.21, which was higher than 12.0. It indicated that soil organic carbon in Shuangtaizi estuarine mainly came from land but not ocean and plants contributed the most of soil organic matters. There was no significant difference in R(C/N) between soil from the four plant communities (F = 1.890, p = 0.151). R(C/N) was related significantly to sol salinity (r = 0.346 3, p = 0.035 8) and was increasing with soil salinity.

Hypothalamic melanin concentrating hormone neurons communicate the nutrient value of sugar

PubMed Central

Domingos, Ana I; Sordillo, Aylesse; Dietrich, Marcelo O; Liu, Zhong-Wu; Tellez, Luis A; Vaynshteyn, Jake; Ferreira, Jozelia G; Ekstrand, Mats I; Horvath, Tamas L; de Araujo, Ivan E; Friedman, Jeffrey M

2013-01-01

Sugars that contain glucose, such as sucrose, are generally preferred to artificial sweeteners owing to their post-ingestive rewarding effect, which elevates striatal dopamine (DA) release. While the post-ingestive rewarding effect, which artificial sweeteners do not have, signals the nutrient value of sugar and influences food preference, the neural circuitry that mediates the rewarding effect of glucose is unknown. In this study, we show that optogenetic activation of melanin-concentrating hormone (MCH) neurons during intake of the artificial sweetener sucralose increases striatal dopamine levels and inverts the normal preference for sucrose vs sucralose. Conversely, animals with ablation of MCH neurons no longer prefer sucrose to sucralose and show reduced striatal DA release upon sucrose ingestion. We further show that MCH neurons project to reward areas and are required for the post-ingestive rewarding effect of sucrose in sweet-blind Trpm5−/− mice. These studies identify an essential component of the neural pathways linking nutrient sensing and food reward. DOI: http://dx.doi.org/10.7554/eLife.01462.001 PMID:24381247

A novel sphingolipid-TORC1 pathway critically promotes postembryonic development in Caenorhabditis elegans

PubMed Central

Zhu, Huanhu; Shen, Huali; Sewell, Aileen K; Kniazeva, Marina; Han, Min

2013-01-01

Regulation of animal development in response to nutritional cues is an intensely studied problem related to disease and aging. While extensive studies indicated roles of the Target of Rapamycin (TOR) in sensing certain nutrients for controlling growth and metabolism, the roles of fatty acids and lipids in TOR-involved nutrient/food responses are obscure. Caenorhabditis elegans halts postembryonic growth and development shortly after hatching in response to monomethyl branched-chain fatty acid (mmBCFA) deficiency. Here, we report that an mmBCFA-derived sphingolipid, d17iso-glucosylceramide, is a critical metabolite in regulating growth and development. Further analysis indicated that this lipid function is mediated by TORC1 and antagonized by the NPRL-2/3 complex in the intestine. Strikingly, the essential lipid function is bypassed by activating TORC1 or inhibiting NPRL-2/3. Our findings uncover a novel lipid-TORC1 signaling pathway that coordinates nutrient and metabolic status with growth and development, advancing our understanding of the physiological roles of mmBCFAs, ceramides, and TOR. DOI: http://dx.doi.org/10.7554/eLife.00429.001 PMID:23705068

Monitoring changes in Greater Yellowstone Lake water quality following the 1988 wildfires

NASA Technical Reports Server (NTRS)

Lathrop, Richard G., Jr.; Vande Castle, John D.; Brass, James A.

1994-01-01

The fires that burned the Greater Yellowstone Area (GYA) during the summer of 1988 were the largest ever recorded for the region. Wildfire can have profound indirect effects on associated aquatic ecosystems by increased nutrient loading, sediment, erosion, and runoff. Satellite remote sensing and water quality sampling were used to compare pre- versus post-fire conditions in the GYA's large oliotrophic (high transparency, low productivity) lakes. Inputs of suspended sediment to Jackson Lake appear to have increased. Yellowstone Lake has not shown any discernable shift in water quality. The insights gained separately from the Landsat Thematic and NOAA Advanced Very High Resolution Radiometer (AVHRR) remote sensing systems, along with conventional in-situ sampling, can be combined into a useful water quality monitoring tool.

Phosphatidic Acid and Lipid Sensing by mTOR

PubMed Central

Foster, David A.

2013-01-01

Mammalian target of rapamycin (mTOR) has been implicated as a sensor of nutrient sufficiency for dividing cells and is activated by essential amino acids and glucose. However, cells also require lipids for membrane biosynthesis. A central metabolite in the synthesis of membrane phospholipids is phosphatidic acid (PA), which is required for the stability and activity of mTOR complexes. While PA is commonly generated by the phospholipase D-catalyzed hydrolysis of phosphatidylcholine, PA is also generated by diacylglycerol kinases and lysophosphatidic acid acyltransferases, which are at the center of phospholipid biosynthesis. It is proposed that the responsiveness of mTOR/TOR to PA evolved as a means for sensing lipid precursors for membrane biosynthesis prior to doubling the mass of a cell and dividing. PMID:23507202

Modern 'junk food' and minimally-processed 'natural food' cafeteria diets alter the response to sweet taste but do not impair flavor-nutrient learning in rats.

PubMed

Palframan, Kristen M; Myers, Kevin P

2016-04-01

Animals learn to prefer and increase consumption of flavors paired with postingestive nutrient sensing. Analogous effects have been difficult to observe in human studies. One possibility is experience with the modern, processed diet impairs learning. Food processing manipulates flavor, texture, sweetness, and nutrition, obscuring ordinary correspondences between sensory cues and postingestive consequences. Over time, a diet of these processed 'junk' foods may impair flavor-nutrient learning. This 'flavor-confusion' hypothesis was tested by providing rats long-term exposure to cafeteria diets of unusual breadth (2 or 3 foods per day, 96 different foods over 3 months, plus ad libitum chow). One group was fed processed foods (PF) with added sugars/fats and manipulated flavors, to mimic the sensory-nutrient properties of the modern processed diet. Another group was fed only 'natural' foods (NF) meaning minimally-processed foods without manipulated flavors or added sugars/fats (e.g., fresh fruits, vegetables, whole grains) ostensibly preserving the ordinary correspondence between flavors and nutrition. A CON group was fed chow only. In subsequent tests of flavor-nutrient learning, PF and NF rats consistently acquired strong preferences for novel nutrient-paired flavors and PF rats exhibited enhanced learned acceptance, contradicting the 'flavor-confusion' hypothesis. An unexpected finding was PF and NF diets both caused lasting reduction in ad lib sweet solution intake. Groups did not differ in reinforcing value of sugar in a progressive ratio task. In lick microstructure analysis the NF group paradoxically showed increased sucrose palatability relative to PF and CON, suggesting the diets have different effects on sweet taste evaluation. Copyright © 2016 Elsevier Inc. All rights reserved.

The Impact of High-Turbidity Water's Seasonal and Decadal Variations on Offshore Phytoplankton and Nutrients Dynamics around The Changjiang Estuary

NASA Astrophysics Data System (ADS)

Ge, J.; Torres, R.; Chen, C.; Bellerby, R. G. J.

2017-12-01

The Changjiang Estuary is characterized as strong river discharge into the inner shelf of the East China Sea with abundant sediment load, producing significant high-turbidity water coverage from river mouth to deep region. The growth of offshore phytoplankton is dynamically controlled by river flushed low-salinity and high-turbidity water, and salter water from inner shelf of East China Sea. During last decade, the sediment and nutrients from the Changjiang River has significantly changed, which lead to the variation of offshore phytoplankton dynamics. The variations of sediment, nutrients, and their influenced phytoplankton has been simulated through a comprehensive modeling system, which integrated a multi-scale current-wave-sediment FVCOM model and generic marine biogeochemistry and ecosystem ERSEM model through The Framework for Aquatic Biogeochemical Models (FABM). This model system has successfully revealed the seasonal and decadal variations of sediment, nutrients transport around the inner shelf of the East China Sea. The spring and autumn peaks of phytoplankton growth were correctly captured by simulation. The modeling results, as well as MODIS and GOCI remote sensing, shows a strong sediment decreasing from northern to southern region, which creates different patterns of Chlorophyll-a distribution and seasonal variations. These results indicate the high-turbidity water in northern region strongly influenced the light attenuation in the water column and limits the phytoplankton growth in this relatively higher-nutrient area, especially in the wintertime. The relatively low-turbidity southern region has significant productivity of phytoplankton, even during low-temperature winter. The phytoplankton growth increased in the northern region from 2005 to 2010, with the increase of the nutrient load during this period. Then it became a decreasing trend after 2010.

Minimising losses to predation during microalgae cultivation.

PubMed

Flynn, Kevin J; Kenny, Philip; Mitra, Aditee

2017-01-01

We explore approaches to minimise impacts of zooplanktonic pests upon commercial microalgal crops using system dynamics models to describe algal growth controlled by light and nutrient availability and zooplankton growth controlled by crop abundance and nutritional quality. Losses of microalgal crops are minimised when their growth is fastest and, in contrast, also when growing slowly under conditions of nutrient exhaustion. In many culture systems, however, dwindling light availability due to self-shading in dense suspensions favours slow growth under nutrient sufficiency. Such a situation improves microalgal quality as prey, enhancing zooplankton growth, and leads to rapid crop collapse. Timing of pest entry is important; crop losses are least likely in established, nutrient-exhausted microalgal communities grown for high C-content (e.g. for biofuels). A potentially useful approach is to promote a low level of P-stress that does not adversely affect microalgal growth but which produces a crop that is suboptimal for zooplankton growth.

Convenience stores are the key food environment influence on nutrients available from household food supplies in Texas Border Colonias

PubMed Central

2013-01-01

Background Few studies have focused on the relationship between the retail food environment and household food supplies. This study examines spatial access to retail food stores, food shopping habits, and nutrients available in household food supplies among 50 Mexican-origin families residing in Texas border colonias. Methods The design was cross-sectional; data were collected in the home March to June 2010 by promotora-researchers. Ground-truthed methods enumerated traditional (supercenters, supermarkets, grocery stores), convenience (convenience stores and food marts), and non-traditional (dollar stores, discount stores) retail food stores. Spatial access was computed using the network distance from each participant’s residence to each food store. Data included survey data and two household food inventories (HFI) of the presence and amount of food items in the home. The Spanish language interviewer-administered survey included demographics, transportation access, food purchasing, food and nutrition assistance program participation, and the 18-item Core Food Security Module. Nutrition Data Systems for Research (NDS-R) was used to calculate HFI nutrients. Adult equivalent adjustment constants (AE), based on age and gender calorie needs, were calculated based on the age- and gender composition of each household and used to adjust HFI nutrients for household composition. Data were analyzed using bivariate analysis and linear regression models to determine the association of independent variables with the availability of each AE-adjusted nutrient. Results Regression models showed that households in which the child independently purchased food from a convenience store at least once a week had foods and beverages with increased amounts of total energy, total fat, and saturated fat. A greater distance to the nearest convenience store was associated with reduced amounts of total energy, vitamin D, total sugar, added sugar, total fat, and saturated fat. Participation in the National School Lunch Program (NSLP) was associated with lower household levels of total energy, calcium, vitamin C, sodium, vitamin D, and saturated fat. Spatial access and utilization of supermarkets and dollar stores were not associated with nutrient availability. Conclusions Although household members frequently purchased food items from supermarkets or dollar stores, it was spatial access to and frequent utilization of convenience food stores that influenced the amount of nutrients present in Texas border colonia households. These findings also suggest that households which participate in NSLP have reduced AE-adjusted nutrients available in the home. The next step will target changes within convenience stores to improve in-store marketing of foods and beverages to children and adults. PMID:23327426

Convenience stores are the key food environment influence on nutrients available from household food supplies in Texas Border Colonias.

PubMed

Sharkey, Joseph R; Dean, Wesley R; Nalty, Courtney C; Xu, Jin

2013-01-17

Few studies have focused on the relationship between the retail food environment and household food supplies. This study examines spatial access to retail food stores, food shopping habits, and nutrients available in household food supplies among 50 Mexican-origin families residing in Texas border colonias. The design was cross-sectional; data were collected in the home March to June 2010 by promotora-researchers. Ground-truthed methods enumerated traditional (supercenters, supermarkets, grocery stores), convenience (convenience stores and food marts), and non-traditional (dollar stores, discount stores) retail food stores. Spatial access was computed using the network distance from each participant's residence to each food store. Data included survey data and two household food inventories (HFI) of the presence and amount of food items in the home. The Spanish language interviewer-administered survey included demographics, transportation access, food purchasing, food and nutrition assistance program participation, and the 18-item Core Food Security Module. Nutrition Data Systems for Research (NDS-R) was used to calculate HFI nutrients. Adult equivalent adjustment constants (AE), based on age and gender calorie needs, were calculated based on the age- and gender composition of each household and used to adjust HFI nutrients for household composition. Data were analyzed using bivariate analysis and linear regression models to determine the association of independent variables with the availability of each AE-adjusted nutrient. Regression models showed that households in which the child independently purchased food from a convenience store at least once a week had foods and beverages with increased amounts of total energy, total fat, and saturated fat. A greater distance to the nearest convenience store was associated with reduced amounts of total energy, vitamin D, total sugar, added sugar, total fat, and saturated fat. Participation in the National School Lunch Program (NSLP) was associated with lower household levels of total energy, calcium, vitamin C, sodium, vitamin D, and saturated fat. Spatial access and utilization of supermarkets and dollar stores were not associated with nutrient availability. Although household members frequently purchased food items from supermarkets or dollar stores, it was spatial access to and frequent utilization of convenience food stores that influenced the amount of nutrients present in Texas border colonia households. These findings also suggest that households which participate in NSLP have reduced AE-adjusted nutrients available in the home. The next step will target changes within convenience stores to improve in-store marketing of foods and beverages to children and adults.

Estimations of daily energy and nutrient availability based on nationally representative household budget survey data. The Data Food Networking (DAFNE) project.

PubMed

Naska, A; Oikonomou, E; Trichopoulou, A; Wagner, K; Gedrich, K

2007-12-01

To describe a cost-efficient method for estimating energy and nutrient availability using household budget survey (HBS) data. Four different approaches were tested and the results were compared with published nutrient intake data. The selected method was exemplarily applied in German and Greek data. Germany, 1998; Greece, 1998/99. Nationally representative HBSs. Comparisons showed that HBS-based estimates were generally close to intake data when results were presented as contributions to daily energy intake. Daily energy and protein availabilities were similar in Germany and Greece. Differences were observed in the availability of carbohydrates (German households reported a 5 percentage points higher contribution to daily energy availability) and lipids (Greek households recorded higher values for total fat, but lower values for saturated fat). Meat, added lipids and potatoes were important energy suppliers in Germany, whereas in Greece the first three energy suppliers were added lipids, cereals and meat. In both countries, meat, cereals, milk and cheese were important protein sources and cereals, potatoes, fruits and nuts contributed more than 60% of the daily carbohydrate availability. Added lipids were the major source of fat in the daily diet of both countries, but their contribution amounted to less than one-third in Germany and two-thirds in Greece. National HBS data can be used for monitoring and comparing nutrient availability among representative population samples of different countries. The ground is set for the development of a harmonised food composition table to be applied to HBS food data at international level.

Manipulating stomatal density enhances drought tolerance without deleterious effect on nutrient uptake.

PubMed

Hepworth, Christopher; Doheny-Adams, Timothy; Hunt, Lee; Cameron, Duncan D; Gray, Julie E

2015-10-01

Manipulation of stomatal density was investigated as a potential tool for enhancing drought tolerance or nutrient uptake. Drought tolerance and soil water retention were assessed using Arabidopsis epidermal patterning factor mutants manipulated to have increased or decreased stomatal density. Root nutrient uptake via mass flow was monitored under differing plant watering regimes using nitrogen-15 ((15) N) isotope and mass spectrometry. Plants with less than half of their normal complement of stomata, and correspondingly reduced levels of transpiration, conserve soil moisture and are highly drought tolerant but show little or no reduction in shoot nitrogen concentrations especially when water availability is restricted. By contrast, plants with over twice the normal density of stomata have a greater capacity for nitrogen uptake, except when water availability is restricted. We demonstrate the possibility of producing plants with reduced transpiration which have increased drought tolerance, with little or no loss of nutrient uptake. We demonstrate that increasing transpiration can enhance nutrient uptake when water is plentiful. © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

Integration of In Situ Radon Modeling with High Resolution Aerial Remote Sensing for Mapping and Quantifying Local to Regional Flow and Transport of Submarine Groundwater Discharge from Coastal Aquifers

NASA Astrophysics Data System (ADS)

Glenn, C. R.; Kennedy, J. J.; Dulaiova, H.; Kelly, J. L.; Lucey, P. G.; Lee, E.; Fackrell, J.

2015-12-01

Submarine groundwater discharge (SGD) is a principal conduit for huge volumes of fresh groundwater loss and is a key transport mechanism for nutrient and contaminant pollution to coastal zones worldwide. However, the volumes and spatially and temporally variable nature of SGD is poorly known and requires rapid and high-resolution data acquisition at the scales in which it is commonly observed. Airborne thermal infrared (TIR) remote sensing, using high-altitude manned aircraft and low-altitude remote-controlled unmanned aerial vehicles (UAVs or "Drones") are uniquely qualified for this task, and applicable wherever 0.1°C temperature contrasts exist between discharging and receiving waters. We report on the use of these technologies in combination with in situ radon model studies of SGD volume and nutrient flux from three of the largest Hawaiian Islands. High altitude manned aircraft results produce regional (~300m wide x 100s km coastline) 0.5 to 3.2 m-resolution sea-surface temperature maps accurate to 0.7°C that show point-source and diffuse flow in exquisite detail. Using UAVs offers cost-effective advantages of higher spatial and temporal resolution and instantaneous deployments that can be coordinated simultaneously with any ground-based effort. We demonstrate how TIR-mapped groundwater discharge plume areas may be linearly and highly correlated to in situ groundwater fluxes. We also illustrate how in situ nutrient data may be incorporated into infrared imagery to produce nutrient distribution maps of regional worth. These results illustrate the potential for volumetric quantification and up-scaling of small- to regional-scale SGD. These methodologies provide a tremendous advantage for identifying and differentiating spring-fed, point-sourced, and/or diffuse groundwater discharge into oceans, estuaries, and streams. The integrative techniques are also important precursors for developing best-use and cost-effective strategies for otherwise time-consuming in situ studies, and represent a substantial new asset for land use and coastal zone research and management.

Connecting landscape function to hyperspectral reflectance in a dry sub-humid native grassland in southern Queensland, Australia

NASA Astrophysics Data System (ADS)

Williams, Wendy; Apan, Armando; Alchin, Bruce

2016-04-01

Native grasslands cover over 80% of significant ecosystems in Australia, stretching across arid, semi-arid, tropical, sub-tropical and savannah landscapes. Scales of pastoral operations in Australia range from hundreds of hectares to thousands of square kilometres and are predominately found in regions with highly variable rainfall. Land use is governed by the need to cope with droughts, floods and fires. Resilience to climatic extremes can be attained through effective soil management. Connecting landscape function on the fine scale to broad land management objectives is a critical step in evaluation and requires an understanding of the relevant spectral properties in remotely sensed images. The aim of this study was to assess key landscape function indices across spatial scales in order to examine their correlation with hyperspectral reflectance measurements. The results from this study could be applied as a model for land management centred on remote sensing. The study site is located at Stonehenge (southern Queensland) on a moderately deep texture contrast soil with hard setting gravelly topsoil. Mean annual rainfall of 667 mm supports open forest and native perennial pastures with a diverse biocrust dominated by N-fixing cyanobacteria. Land use history is continuous grazing however; it had been destocked for several years prior to our study. There was some evidence of cattle, kangaroos and feral herbivores (rabbits, deer and goats) although impacts appeared to be minimal. We established four land cover types: native pasture - NP1 (~100% FPC - foliage projective cover), native pasture - NP2 (~50% FPC, 50% biocrust), natural bare soil - BC (>80% biocrust), bare and eroded soil - BE (<1% biocrust). Duplicate 0.25 m2 quadrats of each land cover type were selected contiguous with a 100 m transect across the slope. The quadrats were analysed as five micro-transects with each row consisting of five sub-cells. Stability, infiltration and nutrient cycling indices were measured in each sub-cell. Hyperspectral data were also collected at an overall and sub-cell level, under wet and dry conditions and, with FPC removed in order to record the presence of biocrusts. For each micro-transect, soil samples were taken at 0-1 cm and 1-5 cm depths for isotopic C and N, C:N ratio, and plant-available N analysis. The results were adapted at a landscape scale to represent whole paddock management. Preliminary results from the hyperspectral data indicate a clear delineation between native pastures, biocrusts and, bare and eroded soil. Landscape function fell away across all indices between NP1 and BE where; stability declined from 70 to 55%; infiltration from 36 to 25% and, nutrient cycling from 29-14%. By tapping into remote sensing, productivity improvements can be gained through targeted management. For example, our results indicate where areas of nutrient deficiencies are identified, productivity could be considerably increased through the reestablishment of biocrusts. Here we will present the results from this study with a model for its application to land management.

Development of complementary feeding recommendations for 12-23-month-old children from low and middle socio-economic status in West Java, Indonesia: contribution of fortified foods towards meeting the nutrient requirement.

PubMed

Fahmida, Umi; Santika, Otte

2016-07-01

Inadequate nutrient intake as part of a complementary feeding diet is attributable to poor feeding practices and poor access to nutritious foods. Household socio-economic situation (SES) has an influence on food expenditure and access to locally available, nutrient-dense foods and fortified foods. This study aimed to develop and compare complementary feeding recommendations (CFR) for 12-23-month-old children in different SES and evaluate the contribution of fortified foods in meeting nutrient requirements. A cross-sectional survey was conducted in low and medium SES households (n 114/group) in urban Bandung district, West Java province, Indonesia. Food pattern, portion size and affordability were assessed, and CFR were developed for the low SES (LSES) and middle SES (MSES) using a linear programming (LP) approach; two models - with and without fortified foods - were run using LP, and the contribution of fortified foods in the final CFR was identified. Milk products, fortified biscuits and manufactured infant cereals were the most locally available and consumed fortified foods in the market. With the inclusion of fortified foods, problem nutrients were thiamin in LSES and folate and thiamin in MSES groups. Without fortified foods, more problem nutrients were identified in LSES, that is, Ca, Fe, Zn, niacin and thiamin. As MSES consumed more fortified foods, removing fortified foods was not possible, because most of the micronutrient-dense foods were removed from their food basket. There were comparable nutrient adequacy and problem nutrients between LSES and MSES when fortified foods were included. Exclusion of fortified foods in LSES was associated with more problem nutrients in the complementary feeding diet.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Espeleta, Javier F.; Cardon, Zoe G.; Mayer, K. Ulrich

Hydro-biogeochemical processes in the rhizosphere regulate nutrient and water availability, and thus ecosystem productivity. We hypothesized that two such processes often neglected in rhizosphere models — diel plant water use and competitive cation exchange — could interact to enhance availability of K + and NH 4 +, both high-demand nutrients. A rhizosphere model with competitive cation exchange was used to investigate how diel plant water use (i.e., daytime transpiration coupled with no nighttime water use, with nighttime root water release, and with nighttime transpiration) affects competitive ion interactions and availability of K + and NH 4 +. Competitive cation exchangemore » enabled lowdemand cations that accumulate against roots (Ca 2+, Mg 2+, Na +) to desorb NH 4 + and K + from soil, generating non-monotonic dissolved concentration profiles (i.e. ‘hotspots’ 0.1–1 cm from the root). Cation accumulation and competitive desorption increased with net root water uptake. Daytime transpiration rate controlled diel variation in NH 4 + and K + aqueous mass, nighttime water use controlled spatial locations of ‘hotspots’, and day-to-night differences in water use controlled diel differences in ‘hotspot’ concentrations. Finally, diel plant water use and competitive cation exchange enhanced NH 4 + and K + availability and influenced rhizosphere concentration dynamics. Demonstrated responses have implications for understanding rhizosphere nutrient cycling and plant nutrient uptake.« less

Saharan Dust Fertilizing Atlantic Ocean and Amazon Rainforest via Long-range Transport and Deposition: A Perspective from Multiyear Satellite Measurements

NASA Astrophysics Data System (ADS)

Yu, H.; Chin, M.; Yuan, T.; Bian, H.; Remer, L. A.; Prospero, J. M.; Omar, A. H.; Winker, D. M.; Yang, Y.; Zhang, Y.; Zhang, Z.; Zhao, C.

2015-12-01

Massive dust emitted from Sahara desert is carried by trade winds across the tropical Atlantic Ocean, reaching the Amazon Rainforest and Caribbean Sea. Airborne dust degrades air quality and interacts with radiation and clouds. Dust falling to land and ocean adds essential nutrients that could increase the productivity of terrestrial and aquatic ecosystems and modulate the biogeochemical cycles and climate. The resultant climate change will feed back on the production of dust in Sahara desert and its subsequent transport and deposition. Understanding the connections among the remote ecosystems requires an accurate quantification of dust transport and deposition flux on large spatial and temporal scales, in which satellite remote sensing can play an important role. We provide the first multiyear satellite-based estimates of altitude-resolved across-Atlantic dust transport and deposition based on eight-year (2007-2014) record of aerosol three-dimensional distributions from the CALIPSO lidar. On a basis of the 8-year average, 179 Tg (million tons) of dust leaves the coast of North Africa and is transported across Atlantic Ocean, of which 102, 20, and 28 Tg of dust is deposited into the tropical Atlantic Ocean, Caribbean Sea, and Amazon Rainforest, respectively. The dust deposition adds 4.3 Tg of iron and 0.1 Tg of phosphorus to the tropical Atlantic Ocean and Caribbean Sea where the productivity of marine ecosystem depends on the availability of these nutrients. The 28 Tg of dust provides about 0.022 Tg of phosphorus to Amazon Rainforest yearly that replenishes the leak of this plant-essential nutrient by rains and flooding, suggesting an important role of Saharan dust in maintaining the productivity of Amazon rainforest on timescales of decades or centuries. We will also discuss seasonal and interannual variations of the dust transport and deposition, and comparisons of the CALIOP-based estimates with model simulations.

Nitrogen availability alters the expression of carnivory in the northern pitcher plant, Sarracenia purpurea.

PubMed

Ellison, Aaron M; Gotelli, Nicholas J

2002-04-02

Atmospheric transport and deposition of nutrients, especially nitrogen, is a global environmental problem with well-documented consequences for ecosystem dynamics. However, monitoring nitrogen deposition is relatively expensive, monitoring stations are widely spaced, and estimates and predicted impacts of nitrogen deposition are currently derived from spatial modeling and interpolation of limited data. Ombrotrophic ("rain-fed") bogs are nutrient-poor ecosystems that are especially sensitive to increasing nutrient input, and carnivorous plants, which are characteristic of these widespread ecosystem types, may be especially sensitive indicators of N deposition. Botanical carnivory is thought to have evolved in nutrient-poor and well-lit habitats such as bogs because the marginal benefits accruing from carnivory exceed the marginal photosynthetic costs associated with the maintenance of carnivorous organs. However, the production of carnivorous organs can be a phenotypically plastic trait. The northern pitcher plant, Sarracenia purpurea, produces leaves specialized for prey capture and nutrient uptake (pitchers) and leaves that are more efficient at photosynthesis (phyllodia). We hypothesized that relative allocation to these two types of leaves reflects ambient nitrogen availability. We manipulated nutrient availability to plants with leaf enrichment and whole-plot fertilization experiments. Increased nitrogen, but not phosphorus, reduced production of pitchers relative to phyllodia; this result provided empirical support for the cost-benefit model of the evolution of botanical carnivory. Because this phenotypic shift in leaf production occurs in ecological time, our results suggest that S. purpurea could be a reliable and inexpensive biological indicator of nitrogen deposition rates. This suggestion is supported by field observations across a geographic gradient of nitrogen deposition.

Nitrogen availability alters the expression of carnivory in the northern pitcher plant, Sarracenia purpurea

PubMed Central

Ellison, Aaron M.; Gotelli, Nicholas J.

2002-01-01

Atmospheric transport and deposition of nutrients, especially nitrogen, is a global environmental problem with well-documented consequences for ecosystem dynamics. However, monitoring nitrogen deposition is relatively expensive, monitoring stations are widely spaced, and estimates and predicted impacts of nitrogen deposition are currently derived from spatial modeling and interpolation of limited data. Ombrotrophic (“rain-fed”) bogs are nutrient-poor ecosystems that are especially sensitive to increasing nutrient input, and carnivorous plants, which are characteristic of these widespread ecosystem types, may be especially sensitive indicators of N deposition. Botanical carnivory is thought to have evolved in nutrient-poor and well-lit habitats such as bogs because the marginal benefits accruing from carnivory exceed the marginal photosynthetic costs associated with the maintenance of carnivorous organs. However, the production of carnivorous organs can be a phenotypically plastic trait. The northern pitcher plant, Sarracenia purpurea, produces leaves specialized for prey capture and nutrient uptake (pitchers) and leaves that are more efficient at photosynthesis (phyllodia). We hypothesized that relative allocation to these two types of leaves reflects ambient nitrogen availability. We manipulated nutrient availability to plants with leaf enrichment and whole-plot fertilization experiments. Increased nitrogen, but not phosphorus, reduced production of pitchers relative to phyllodia; this result provided empirical support for the cost–benefit model of the evolution of botanical carnivory. Because this phenotypic shift in leaf production occurs in ecological time, our results suggest that S. purpurea could be a reliable and inexpensive biological indicator of nitrogen deposition rates. This suggestion is supported by field observations across a geographic gradient of nitrogen deposition. PMID:11904363

Seasonal amounts of nutrients in Western cherry fruit fly (Diptera: Tephritidae) and their relation to nutrient availability on cherry plant surfaces.

PubMed

Yee, Wee L; Chapman, Peter S

2008-10-01

Relatively little is known about the nutritional ecology of fruit flies in the genus Rhagoletis. In this study, nutrient amounts in male and female western cherry fruit fly, Rhagoletis indifferens Curran, and availability of nitrogen and sugar on surfaces of leaves, fruit, and extrafloral nectaries (EFNs) of sweet cherry trees, were determined from late May to late June 2005 and of sugar from EFNs from mid-May to late June 2007 in Washington state. Protein amounts in male and female flies did not differ over the season. Nitrogen was present on leaves, fruit, and EFNs during the sampling period, but amounts on leaves and fruit were lower in late May than the rest of the season. Sugar amounts in flies did not differ over the season. Sugar was present on leaf, fruit, and EFN surfaces all season, but amounts on all three were lower in late May than later in the season. Fructose and glucose were the predominant sugars on all plant surfaces, but sucrose was also present in nectar from EFNs. In outdoor and field cage experiments in 2004 and 2006, more flies survived when cherry branches with leaves and fruit were present than absent. Results suggest that R. indifferens maintains stable protein and sugar levels throughout the season because sufficient amounts of nutrients are found in cherry trees during this time and that increases in nutrient availability caused by ripening and damaged cherries later in the season do not result in increased amounts of nutrients in flies.

Nutrient balance affects foraging behaviour of a trap-building predator

PubMed Central

Mayntz, David; Toft, Søren; Vollrath, Fritz

2009-01-01

Predator foraging may be affected by previous prey capture, but it is unknown how nutrient balance affects foraging behaviour. Here, we use a trap-building predator to test whether nutrients from previous prey captures affect foraging behaviour. We fed orb-weaving spiders (Zygiella x-notata) prey flies of different nutrient composition and in different amounts during their first instar and measured the subsequent frequency of web building and aspects of web architecture. We found that both the likelihood of web building and the number of radii in the web were affected by prey nutrient composition while prey availability affected capture area and mesh height. Our results show that both the balance of nutrients in captured prey and the previous capture rate may affect future foraging behaviour of predators. PMID:19640870

N and P as ultimate and proximate limiting nutrients in the northern Gulf of Mexico: implications for hypoxia reduction strategies

NASA Astrophysics Data System (ADS)

Fennel, Katja; Laurent, Arnaud

2018-05-01

The occurrence of hypoxia in coastal oceans is a long-standing and growing problem worldwide and is clearly linked to anthropogenic nutrient inputs. While the need for reducing anthropogenic nutrient loads is generally accepted, it is costly and thus requires scientifically sound nutrient-reduction strategies. Issues under debate include the relative importance of nitrogen (N) and phosphorus (P) as well as the magnitude of the reduction requirements. The largest anthropogenically induced hypoxic area in North American coastal waters (of 15 000 ± 5000 km2) forms every summer in the northern Gulf of Mexico where the Mississippi and Atchafalaya rivers deliver large amounts of freshwater and nutrients to the shelf. A 2001 plan for reducing this hypoxic area by nutrient management in the watershed called for a reduction of N loads. Since then evidence of P limitation during the time of hypoxia formation has arisen, and a dual nutrient-reduction strategy for this system has been endorsed. Here we report the first systematic analysis of the effects of single and dual nutrient load reductions from a spatially explicit physical-biogeochemical model for the northern Gulf of Mexico. The model has been shown previously to skillfully represent the processes important for hypoxic formation. Our analysis of an ensemble of simulations with stepwise reductions in N, P, and N and P loads provides insight into the effects of both nutrients on primary production and hypoxia, and it allows us to estimate what nutrient reductions would be required for single and dual nutrient-reduction strategies to reach the hypoxia target. Our results show that, despite temporary P limitation, N is the ultimate limiting nutrient for primary production in this system. Nevertheless, a reduction in P load would reduce hypoxia because primary production is P limited in the region where density stratification is conducive to hypoxia development, but reductions in N load have a bigger effect. Our simulations show that, at present loads, the system is almost saturated with N, in the sense that the sensitivity of primary production and hypoxia to N load is much lower than it would be at lower N loads. We estimate that reductions of 63±18 % in total N load or 48±21 % in total N and P load are necessary to reach a hypoxic area of 5000 km2, which is consistent with previous estimates from statistical regression models and highly simplified mechanistic models.

Long-term fertilization determines different metabolomic profiles and responses in saplings of three rainforest tree species with different adult canopy position.

PubMed

Gargallo-Garriga, Albert; Wright, S Joseph; Sardans, Jordi; Pérez-Trujillo, Míriam; Oravec, Michal; Večeřová, Kristýna; Urban, Otmar; Fernández-Martínez, Marcos; Parella, Teodor; Peñuelas, Josep

2017-01-01

Tropical rainforests are frequently limited by soil nutrient availability. However, the response of the metabolic phenotypic plasticity of trees to an increase of soil nutrient availabilities is poorly understood. We expected that increases in the ability of a nutrient that limits some plant processes should be detected by corresponding changes in plant metabolome profile related to such processes. We studied the foliar metabolome of saplings of three abundant tree species in a 15 year field NPK fertilization experiment in a Panamanian rainforest. The largest differences were among species and explained 75% of overall metabolome variation. The saplings of the large canopy species, Tetragastris panamensis, had the lowest concentrations of all identified amino acids and the highest concentrations of most identified secondary compounds. The saplings of the "mid canopy" species, Alseis blackiana, had the highest concentrations of amino acids coming from the biosynthesis pathways of glycerate-3P, oxaloacetate and α-ketoglutarate, and the saplings of the low canopy species, Heisteria concinna, had the highest concentrations of amino acids coming from the pyruvate synthesis pathways. The changes in metabolome provided strong evidence that different nutrients limit different species in different ways. With increasing P availability, the two canopy species shifted their metabolome towards larger investment in protection mechanisms, whereas with increasing N availability, the sub-canopy species increased its primary metabolism. The results highlighted the proportional distinct use of different nutrients by different species and the resulting different metabolome profiles in this high diversity community are consistent with the ecological niche theory.

Rooting depth distribution and nitrogen acquisition using 15N tracer, Barrow, Alaska, 2013

DOE Office of Scientific and Technical Information (OSTI.GOV)

Colleen Iversen

Permafrost thaw and degradation may lead to altered thickness of the active soil layer and a changing distribution of plant-available nutrients throughout the soil, but little is known about the nutrient acquisition strategies of dominant tundra plant species. We conducted an 15N isotope tracer experiment to assess the vertical distribution of nutrient acquisition among three dominant species representing important plant functional types (PFTs) on the Barrow Environmental Observatory (BEO) in Barrow, Alaska. We found that vertical patterns of root distribution and nutrient acquisition varied among plant species, and that root density may not entirely explain patterns of nutrient acquisition formore » all species.« less

Fronts and eddies: Engines for biogeochemical variability of the Central Red Sea during winter-spring periods

NASA Astrophysics Data System (ADS)

Zarokanellos, Nikolaos; Jones, Burton

2017-04-01

The central Red Sea (CRS) has been shown to be characterized by significant eddy activity throughout the year. In winter, weakened stratification may lead to enhanced vertical exchange contributing to physical and biogeochemical processes. In winter 2014-2015 we began an extended glider time series to monitor a region in the northern CRS where eddy activity is significant. Remote sensing and glider observations that include CTD, oxygen, CDOM and chlorophyll fluorescence, and multi-wavelength optical backscatter, have been used to characterize the effects of winter mixing and eddy activity in this region. During winter, deep mixing driven by surface cooling and strong winds combined with eddy features, can supply nutrients into the upper layer dramatically modifies the environment from its typically stratified conditions. These mixing events disperse the phytoplankton from the deep chlorophyll maximum throughout the upper mixed layer, and increase the chlorophyll signature detected by ocean color imagery. In addition to the mixing, cyclonic eddies in the region can enhance the vertical displacement of deeper, nutrient containing water toward the euphotic zone contributing to increased chlorophyll concentration and biological productivity. Remote sensing analyses indicate that these eddies also contribute to significant horizontal dispersion including the exchange between the open sea and coastal coral reef ecosystems. During the winter mixing periods, diel fluctuations in phytoplankton biomass have been observed indicative of solar driven plankton dynamics. The biogeochemical response to the subsurface physical processes provides a sensitive indicator to the processes that result from the mixing and eddy dynamics - processes that are not necessarily detectable via remote sensing. In order to understand the seasonal responses, but also the interannual influences on these processes, sustained in situ autonomous platform measurements are essential.

Role of nutrient-sensing taste 1 receptor (T1R) family members in gastrointestinal chemosensing.

PubMed

Shirazi-Beechey, Soraya P; Daly, Kristian; Al-Rammahi, Miran; Moran, Andrew W; Bravo, David

2014-06-01

Luminal nutrient sensing by G-protein-coupled receptors (GPCR) expressed on the apical domain of enteroendocrine cells activates intracellular pathways leading to secretion of gut hormones that control vital physiological processes such as digestion, absorption, food intake and glucose homeostasis. The taste 1 receptor (T1R) family of GPCR consists of three members: T1R1; T1R2; T1R3. Expression of T1R1, T1R2 and T1R3 at mRNA and protein levels has been demonstrated in the intestinal tissue of various species. It has been shown that T1R2-T1R3, in association with G-protein gustducin, is expressed in intestinal K and L endocrine cells, where it acts as the intestinal glucose (sweet) sensor. A number of studies have demonstrated that activation of T1R2-T1R3 by natural sugars and artificial sweeteners leads to secretion of glucagon-like peptides 1&2 (GLP-1 and GLP-2) and glucose dependent insulinotropic peptide (GIP). GLP-1 and GIP enhance insulin secretion; GLP-2 increases intestinal growth and glucose absorption. T1R1-T1R3 combination co-expressed on the apical domain of cholecystokinin (CCK) expressing cells is a luminal sensor for a number of L-amino acids; with amino acid-activation of the receptor eliciting CCK secretion. This article focuses on the role of the gut-expressed T1R1, T1R2 and T1R3 in intestinal sweet and L-amino acid sensing. The impact of exploiting T1R2-T1R3 as a nutritional target for enhancing intestinal glucose absorption and gut structural maturity in young animals is also highlighted.

Plant component features of forest-bog ecotones of eutrophic paludification in the south of boreal forest zone of West Siberia

NASA Astrophysics Data System (ADS)

Klimova, N. V.; Chernova, N. A.; Pologova, N. N.

2018-03-01

Paludified forests formed in transitional forest-bog zone aren’t studied enough, inspite of its high expected diversity and large areas in the south of boreal forest zone of West Siberia. In this article wet birch (Betula pubescens) forests of forest-bog ecotones of eutrophic paludification are investigated on Vasyugan plain with nutrient-rich calcareous clays as soil-forming rocks. Species diversity and ecocoenotic structure of these phytocoenoses are discussed. They correlated with wetness and nutrient-availability of habitats evaluated with indicator values of plants. The participation of hydrophylous species is increasing as wetness of habitats increasing in the forest-to-bog direction like in mesotrophic paludification series. However the number of species is higher in the phytocoenoses of eutrophic paludification. The share of species required to nutrient availability is also higher, both in number and in abundance. A lot of these species are usual for eutrophic boreal forested swamps with groundwater input and absent in forests of mesotrophic paludification. Accordingly the nutrient-availability of habitats is also higher. All these features we connect with birch to be a forest forming species instead of dark-coniferous and with the influence of nutrient-rich parent rocks, which is evident in forest-bog ecotones of Vasyugan plain gradually decreasing together with peat horizon thickening.

Effects of flooding on ion exchange rates in an Upper Mississippi River floodplain forest impacted by herbivory, invasion, and restoration

USGS Publications Warehouse

Kreiling, Rebecca; DeJager, Nathan R.; Whitney Swanson,; Eric A. Strauss,; Meredith Thomsen,

2015-01-01

We examined effects of flooding on supply rates of 14 nutrients in floodplain areas invaded by Phalaris arundinacea (reed canarygrass), areas restored to young successional forests (browsed by white-tailed deer and unbrowsed), and remnant mature forests in the Upper Mississippi River floodplain. Plant Root Simulator ion-exchange probes were deployed for four separate 28-day periods. The first deployment occurred during flooded conditions, while the three subsequent deployments were conducted during progressively drier periods. Time after flooding corresponded with increases in NO3 −-N, K+ and Zn+2, decreases in H2PO4 −-P, Fe+3, Mn+2, and B(OH)4-B, a decrease followed by an increase in NH4 +-N, Ca+2, Mg+2 and Al+3, and an increase followed by a decrease for SO4 −2-S. Plant community type had weak to no effects on nutrient supply rates compared to the stronger effects of flooding duration. Our results suggest that seasonal dynamics in floodplain nutrient availability are similarly driven by flood pulses in different community types. However, reed canarygrass invasion has potential to increase availability of some nutrients, while restoration of forest cover may promote recovery of nutrient availability to that observed in reference mature forests.

Contrasting Strategies of Photosynthetic Energy Utilization Drive Lifestyle Strategies in Ecologically Important Picoeukaryotes

PubMed Central

Halsey, Kimberly H.; Milligan, Allen J.; Behrenfeld, Michael J.

2014-01-01

The efficiency with which absorbed light is converted to net growth is a key property for estimating global carbon production. We previously showed that, despite considerable evolutionary distance, Dunaliella tertiolecta (Chlorophyceae) and Thalassiosira weissflogii (Bacillariophyceae) share a common strategy of photosynthetic energy utilization and nearly identical light energy conversion efficiencies. These findings suggested that a single model might be appropriate for describing relationships between measures of phytoplankton production. This conclusion was further evaluated for Ostreococcus tauri RCC1558 and Micromonas pusilla RCC299 (Chlorophyta, Prasinophyceae), two picoeukaryotes with contrasting geographic distributions and swimming abilities. Nutrient-dependent photosynthetic efficiencies in O. tauri were similar to the previously studied larger algae. Specifically, absorption-normalized gross oxygen and carbon production and net carbon production were independent of nutrient limited growth rate. In contrast, all measures of photosynthetic efficiency were strongly dependent on nutrient availability in M. pusilla. This marked difference was accompanied by a diminished relationship between Chla:C and nutrient limited growth rate and a remarkably greater efficiency of gross-to-net energy conversion than the other organisms studied. These results suggest that the cost-benefit of decoupling pigment concentration from nutrient availability enables motile organisms to rapidly exploit more frequent encounters with micro-scale nutrient patches in open ocean environments. PMID:24957026

Viral pathogen production in a wild grass host driven by host growth and soil nitrogen.

PubMed

Whitaker, Briana K; Rúa, Megan A; Mitchell, Charles E

2015-08-01

Nutrient limitation is a basic ecological constraint that has received little attention in studies on virus production and disease dynamics. Nutrient availability could directly limit the production of viral nucleic acids and proteins, or alternatively limit host growth and thus indirectly limit metabolic pathways necessary for viral replication. In order to compare direct and indirect effects of nutrient limitation on virus production within hosts, we manipulated soil nitrogen (N) and phosphorus (P) availability in a glasshouse for the wild grass host Bromus hordeaceus and the viral pathogen Barley yellow dwarf virus-PAV. We found that soil N additions increased viral concentrations within host tissues, and the effect was mediated by host growth. Specifically, in statistical models evaluating the roles of host biomass production, leaf N and leaf P, viral production depended most strongly on host biomass, rather than the concentration of either nutrient. Furthermore, at low soil N, larger plants supported greater viral concentrations than smaller ones, whereas at high N, smaller plants supported greater viral concentrations. Our results suggest that enhanced viral productivity under N enrichment is an indirect consequence of nutrient stimulation to host growth rate. Heightened pathogen production in plants has important implications for a world facing increasing rates of nutrient deposition. © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

Contribution of Nutrient Diversity and Food Perceptions to Food and Nutrition Security Among Smallholder Farming Households in Western Kenya: A Case Study.

PubMed

Ng'endo, Mary; Bhagwat, Shonil; Keding, Gudrun B

2018-03-01

Sub-Saharan Africa is the only region in the world where hunger is prevalent in over one-third of the population, with smallholder farming households, producers of over 80% of Africa's food, facing both calorie and micronutrient deficiencies. With agricultural systems serving as the main source of all nutrients, little is known about the extent to which agricultural diversity in different seasons can meet macro- and micronutrient needs in rural Africa. Linkages between nutrient diversity and food species were investigated. A case study was conducted in Western Kenya to assess the seasonal nutrient diversity, seasonal nutrient accessibility levels, and food perceptions in 30 smallholder farms, 7 markets, and among 97 focus group discussion participants, respectively. All present food plant and animal species were inventoried and assigned to 1 of the 7 major Food and Agriculture Organization-defined food groups. Based on 2 macronutrients and 5 micronutrients, dendrogram-based nutrient functional diversity metrics were calculated. On-farm and market food species offered all 7 macro- and micronutrients under investigation, regardless of seasonal variation in species numbers. Although there were varying seasonal nutrient accessibility levels in markets, farms were especially effective in readily availing 4 of the 7 nutrients. However, the main food shortage months coincided only with maize shortage, but a diversity of local foods, deemed to be of low cultural and culinary preferences, were available. Nutrition education on the importance of a diversity of local foods in meeting dietary needs, thus stimulating the demand side, can contribute to achieving year-round household food security.

[Changes in bio-availability of immobilized Cu and Zn bound to phosphate in contaminated soils with different nutrient addition].

PubMed

Xu, Ming-Gang; Zhang, Qian; Sun, Nan; Shen, Hua-Ping; Zhang, Wen-Ju

2009-07-15

Bio-availability of Cu and Zn fixed by phosphate in contaminated soils with application of nutrients were measured by pot experiment. It was simulated for the third national standardization of copper and zinc polluted soils by adding copper and zinc nitrate into red and paddy soils, respectively and together. Phosphate amendment was added to the soils to fix Cu and Zn, then added KCl and NH4Cl or K2SO4 and (NH4)2SO4 fertilizers following to plant Ryegrass, which was harvested after 40 d. Available Cu/Zn content in soils and biomass, Cu/Zn content in the shoot of Ryegrass were determined. Results showed that, compared with no nutrient application, adding KCl and NH4 Cl/K2SO4 and (NH4)2SO4 to polluted red and paddy soils increased the available Cu and Zn content in red soil significantly. The increasing order was KCl and NH4 Cl > K2SO4 and (NH4)2SO4. Especially in single Zn polluted red soil, the available Zn content increased by 133.4% in maximum. Although adding K2SO4 and (NH4)2SO4 could promote the growth of Ryegrass on red soil, and the largest increasing was up to 22.2%, it increased Cu and Zn content in the shoot of Ryegrass for 21.5%-112.6% remarkably. These nutrient effects on available Cu and Zn were not significantly in paddy soil. It was suggested that application of nitrogen and potassium fertilizers to soils could change the bioavailability of Cu/Zn. So it is necessary to take full account of the nutrient influence to the heavy metal stability which fixed by phosphate in contaminated soils when consider contaminated soils remediation by fertilization.

A new conceptual framework for unifying the heterogeneity of plant-microbe interactions in forests by linking belowground measurements with large-scale modeling and remote sensing

NASA Astrophysics Data System (ADS)

Brzostek, E. R.; Phillips, R.; Fisher, J. B.

2015-12-01

Recognition of the importance of rhizosphere interactions to ecosystem processes has led to efforts to integrate these dynamics into a conceptual framework that can be tested, refined and applied across spatial scales. A new view suggests that a plant's mycorrhizal association represents a "trait integrator" for a suite of aboveground and belowground functional traits involved in coupling C-nutrient cycles, since nearly all plants associate with a single type of mycorrhizal fungi. The MANE framework predicts that tree species that associate with arbuscular mycorrhizal (AM) fungi differ from trees that associate with ectomycorrhizal (ECM) fungi in a suite of functional traits, and that such differences contribute to unique "biogeochemical syndromes" in forests with varying abundances of AM- and ECM-associated trees. To date, we have found that relative to AM trees, the leaf litter of ECM trees decomposes nearly 50% more slowly; as such, the nutrient economy of ECM-dominated stands is driven by organic forms of N and P whereas the nutrient economy of AM-dominated stands in driven by inorganic forms of N and P. Moreover, differences in the nutrient economies between AM- and ECM-dominated stands can affect the carbon (C) cost of nutrient acquisition. For example, while ECM trees allocate 2-3-fold more C to fine roots and mycorrhizal fungi, this greater investment results in the enhanced activity of enzymes that mobilize nitrogen (N) and phosphorus (P) from soil organic matter, and ultimately the greater uptake of nutrients by plants. However, this enhanced uptake by ECM trees comes at a cost to soil organic C, which declines as a function of root-accelerated N mineralization. By incorporating these dynamics into a coupled nutrient acquisition-microbial decomposition model, and scaling these processes following development of a map of mycorrhizal associations, we are now quantifying how belowground processes shape ecosystem sensitivity to global changes (e.g., rising CO2, warming) at regional- and continental-scales.

Designing a high-frequency nutrient and biogeochemical monitoring network for the Sacramento–San Joaquin Delta, northern California

USGS Publications Warehouse

Bergamaschi, Brian A.; Downing, Bryan D.; Kraus, Tamara E.C.; Pellerin, Brian A.

2017-07-11

Executive SummaryThis report is the third in a series of three reports that provide information about how high-frequency (HF) nutrient monitoring may be used to assess nutrient inputs and dynamics in the Sacramento–San Joaquin Delta, California (Delta). The purpose of this report is to provide the background, principles, and considerations for designing an HF nutrient-monitoring network for the Delta to address high-priority, nutrient-management questions. The report starts with discussion of the high-priority management questions to be addressed, continues through discussion of the questions and considerations that place demands and constraints on network design, discusses the principles applicable to network design, and concludes with the presentation of three example nutrient-monitoring network designs for the Delta. For three example network designs, we assess how they would address high-priority questions that have been identified by the Delta Regional Monitoring Program (Delta Regional Monitoring Program Technical Advisory Committee, 2015).This report, along with the other two reports of this series (Kraus and others, 2017; Downing and others, 2017), was drafted in cooperation with the Delta Regional Monitoring Program to help scientists, managers, and planners understand how HF data improve our understanding of nutrient sources and sinks, drivers, and effects in the Delta. The first report in the series (Kraus and others, 2017) provides an introduction to the reasons for and fundamental concepts behind using HF monitoring measurements, including a brief summary of nutrient status and trends in the Delta and an extensive literature review showing how and where other research and monitoring programs have used HF monitoring to improve our understanding of nutrient cycling. The report covers the various technologies available for HF nutrient monitoring and presents the different ways HF monitoring instrumentation may be used for both fixed station and spatial assessments. Finally, it presents numerous examples of how HF measurements are currently (2017) being used in the Delta to examine how nutrients and nutrient cycling are related to aquatic habitat conditions.The second report in the series (Downing and others, 2017) summarizes information about HF nutrient and associated biogeochemical monitoring in the north Delta. The report synthesizes data available from the nutrient and water quality monitoring network currently (2017) operated by the U.S. Geological Survey in this ecologically important region of the Delta. In the report, we present and discuss the available data at various timescales—first at the monthly, seasonal, and inter-annual timescales; and, second, for comparison, at the tidal and event timescales. As expected, we determined that there is substantial variability in nitrate concentrations at short timescales, such as within a few hours, but also significant variability at longer timescales such as months or years. This high variability affects calculation of fluxes and loads, indicating that HF monitoring is necessary for understanding and assessing flux-based processes and outcomes in Delta tidal environments.

Peatland simulator connecting drainage, nutrient cycling, forest growth, economy and GHG efflux in boreal and tropical peatlands

NASA Astrophysics Data System (ADS)

Lauren, Ari; Hökkä, Hannu; Launiainen, Samuli; Palviainen, Marjo; Lehtonen, Aleksi

2016-04-01

Forest growth in peatlands is nutrient limited; principal source of nutrients is the decomposition of organic matter. Excess water decreases O2 diffusion and slows down the nutrient release. Drainage increases organic matter decomposition, CO2 efflux, and nutrient supply, and enhances the growth of forest. Profitability depends on costs, gained extra yield and its allocation into timber assortments, and the rate of interest. We built peatland simulator Susi to define and parameterize these interrelations. We applied Susi-simulator to compute water and nutrient processes, forest growth, and CO2 efflux of forested drained peatland. The simulator computes daily water fluxes and storages in two dimensions for a peatland forest strip located between drainage ditches. The CO2 efflux is made proportional to peat bulk density, soil temperature and O2 availability. Nutrient (N, P, K) release depends on decomposition and peat nutrient content. Growth limiting nutrient is detected by comparing the need and supply of nutrients. Increased supply of growth limiting nutrient is used to quantify the forest growth response to improved drainage. The extra yield is allocated into pulpwood and sawlogs based on volume of growing stock. The net present values of ditch cleaning operation and the gained extra yield are computed under different rates of interest to assess the profitability of the ditch cleaning. The hydrological sub-models of Susi-simulator were first parameterized using daily water flux data from Hyytiälä SMEAR II-site, after which the predictions were tested against independent hydrologic data from two drained peatland forests in Southern Finland. After verification of the hydrologic model, the CO2 efflux, nutrient release and forest growth proportionality hypothesis was tested and model performance validated against long-term forest growth and groundwater level data from 69 forested peatland sample plots in Central Finland. The results showed a clear relation between the stand growth, nutrient availability, and CO2 efflux. Potassium was the main limiting factor for the forest growth. This indicates that management aiming at decreasing heterotrophic CO2 efflux by raising the ground water table will decrease the forest growth. From the C balance perspective the growth rate of the tree stand becomes essential. Modelling approach enables a search for an optimal management schedule for producing timber in situation when there is a price given for release of C. Ditch network maintenance by ditch cleaning becomes profitable if: i) the initial drainage is very poor, ii) the availability of the critical nutrient is sufficient, iii) during prolonged rainy conditions, and iv) the tree stand is Scots pine (Pinus sylvestris) dominated and v) in a phase where most of the extra yield is allocated into sawlogs. The simulator and its holistic approach has been successfully implemented in both tropical pulpwood plantations in Sumatra, Indonesia and in Finnish boreal forests.

Climate and soil-age constraints on nutrient uplift and retention by plants.

PubMed

Porder, Stephen; Chadwick, Oliver A

2009-03-01

Plants and soils represent coevolving components of ecosystems, and while the effects of soils (e.g., nutrient availability) on plants have been extensively documented, the effect of plants on soils has received less attention. Furthermore there has been no systematic investigation of how plant effects vary across important ecological gradients in climate or soil age, which leaves a substantial gap in our understanding of how plant-soil systems develop. In this context, we analyzed changes in nutrient availability and elemental losses from the entire weathering zone at 35 sites arrayed across climatic and soil-age gradients on the island of Hawai'i. The sites are located on three basaltic lava flows (ages 10, 170, and 350 kyr) each of which crosses a precipitation gradient from approximately 500 to 2500 mm/yr. By comparing the loss of nutrient (potassium, phosphorus) and non-nutrient (e.g., sodium) rock-derived elements, we identify a climatic zone at intermediate rainfall where the retention of plant nutrients in the upper soil is most pronounced. We further show that there are several abiotic constraints on plant-driven retention of nutrients. At the dry sites (< or = 750 mm/yr on all three flows), plants slow the loss of nutrients, but the effect (as measured by the difference between K and Na losses) is small, perhaps because of low plant cover and productivity. At intermediate rainfall (750-1400 mm/yr) but negative water balance, plants substantially enrich both nutrient cations and P relative to Na in the surface horizons, an effect that remains strong even after 350 kyr of soil development. In contrast, at high rainfall (> or = 1500 mm/yr) and positive water balance, the effect of plants on nutrient distributions diminishes with soil age as leaching losses overwhelm the uplift and retention of nutrients by plants after 350 kyr of soil development. The effect of plants on soil nutrient distributions can also be mediated by the movement of iron (Fe), and substantial Fe losses at high rainfall on the older flows are highly correlated with P losses. Thus redox-driven redistribution of Fe may place a further abiotic constraint on nutrient retention by plants. In combination, these data indicate that the effects of soil aging on plant uplift and retention of nutrients differ markedly with precipitation, and we view this as a potentially fruitful area for future research.

Diagnosing oceanic nutrient deficiency

PubMed Central

2016-01-01

The supply of a range of nutrient elements to surface waters is an important driver of oceanic production and the subsequent linked cycling of the nutrients and carbon. Relative deficiencies of different nutrients with respect to biological requirements, within both surface and internal water masses, can be both a key indicator and driver of the potential for these nutrients to become limiting for the production of new organic material in the upper ocean. The availability of high-quality, full-depth and global-scale datasets on the concentrations of a wide range of both macro- and micro-nutrients produced through the international GEOTRACES programme provides the potential for estimation of multi-element deficiencies at unprecedented scales. Resultant coherent large-scale patterns in diagnosed deficiency can be linked to the interacting physical–chemical–biological processes which drive upper ocean nutrient biogeochemistry. Calculations of ranked deficiencies across multiple elements further highlight important remaining uncertainties in the stoichiometric plasticity of nutrient ratios within oceanic microbial systems and caveats with regards to linkages to upper ocean nutrient limitation. This article is part of the themed issue ‘Biological and climatic impacts of ocean trace element chemistry’. PMID:29035255

Grassland productivity limited by multiple nutrients.

PubMed

Fay, Philip A; Prober, Suzanne M; Harpole, W Stanley; Knops, Johannes M H; Bakker, Jonathan D; Borer, Elizabeth T; Lind, Eric M; MacDougall, Andrew S; Seabloom, Eric W; Wragg, Peter D; Adler, Peter B; Blumenthal, Dana M; Buckley, Yvonne M; Chu, Chengjin; Cleland, Elsa E; Collins, Scott L; Davies, Kendi F; Du, Guozhen; Feng, Xiaohui; Firn, Jennifer; Gruner, Daniel S; Hagenah, Nicole; Hautier, Yann; Heckman, Robert W; Jin, Virginia L; Kirkman, Kevin P; Klein, Julia; Ladwig, Laura M; Li, Qi; McCulley, Rebecca L; Melbourne, Brett A; Mitchell, Charles E; Moore, Joslin L; Morgan, John W; Risch, Anita C; Schütz, Martin; Stevens, Carly J; Wedin, David A; Yang, Louie H

2015-07-06

Terrestrial ecosystem productivity is widely accepted to be nutrient limited(1). Although nitrogen (N) is deemed a key determinant of aboveground net primary production (ANPP)(2,3), the prevalence of co-limitation by N and phosphorus (P) is increasingly recognized(4-8). However, the extent to which terrestrial productivity is co-limited by nutrients other than N and P has remained unclear. Here, we report results from a standardized factorial nutrient addition experiment, in which we added N, P and potassium (K) combined with a selection of micronutrients (K+μ), alone or in concert, to 42 grassland sites spanning five continents, and monitored ANPP. Nutrient availability limited productivity at 31 of the 42 grassland sites. And pairwise combinations of N, P, and K+μ co-limited ANPP at 29 of the sites. Nitrogen limitation peaked in cool, high latitude sites. Our findings highlight the importance of less studied nutrients, such as K and micronutrients, for grassland productivity, and point to significant variations in the type and degree of nutrient limitation. We suggest that multiple-nutrient constraints must be considered when assessing the ecosystem-scale consequences of nutrient enrichment.