Sample records for sequencing final technical

  1. A Simulation of DNA Sequencing Utilizing 3M Post-It[R] Notes

    ERIC Educational Resources Information Center

    Christensen, Doug

    2009-01-01

    An inexpensive and equipment free approach to teaching the technical aspects of DNA sequencing. The activity described requires an instructor with a familiarity of DNA sequencing technology but provides a straight forward method of teaching the technical aspects of sequencing in the absence of expensive sequencing equipment. The final sequence…

  2. Identification of usual interstitial pneumonia pattern using RNA-Seq and machine learning: challenges and solutions.

    PubMed

    Choi, Yoonha; Liu, Tiffany Ting; Pankratz, Daniel G; Colby, Thomas V; Barth, Neil M; Lynch, David A; Walsh, P Sean; Raghu, Ganesh; Kennedy, Giulia C; Huang, Jing

    2018-05-09

    We developed a classifier using RNA sequencing data that identifies the usual interstitial pneumonia (UIP) pattern for the diagnosis of idiopathic pulmonary fibrosis. We addressed significant challenges, including limited sample size, biological and technical sample heterogeneity, and reagent and assay batch effects. We identified inter- and intra-patient heterogeneity, particularly within the non-UIP group. The models classified UIP on transbronchial biopsy samples with a receiver-operating characteristic area under the curve of ~ 0.9 in cross-validation. Using in silico mixed samples in training, we prospectively defined a decision boundary to optimize specificity at ≥85%. The penalized logistic regression model showed greater reproducibility across technical replicates and was chosen as the final model. The final model showed sensitivity of 70% and specificity of 88% in the test set. We demonstrated that the suggested methodologies appropriately addressed challenges of the sample size, disease heterogeneity and technical batch effects and developed a highly accurate and robust classifier leveraging RNA sequencing for the classification of UIP.

  3. Reproducibility and quantitation of amplicon sequencing-based detection

    PubMed Central

    Zhou, Jizhong; Wu, Liyou; Deng, Ye; Zhi, Xiaoyang; Jiang, Yi-Huei; Tu, Qichao; Xie, Jianping; Van Nostrand, Joy D; He, Zhili; Yang, Yunfeng

    2011-01-01

    To determine the reproducibility and quantitation of the amplicon sequencing-based detection approach for analyzing microbial community structure, a total of 24 microbial communities from a long-term global change experimental site were examined. Genomic DNA obtained from each community was used to amplify 16S rRNA genes with two or three barcode tags as technical replicates in the presence of a small quantity (0.1% wt/wt) of genomic DNA from Shewanella oneidensis MR-1 as the control. The technical reproducibility of the amplicon sequencing-based detection approach is quite low, with an average operational taxonomic unit (OTU) overlap of 17.2%±2.3% between two technical replicates, and 8.2%±2.3% among three technical replicates, which is most likely due to problems associated with random sampling processes. Such variations in technical replicates could have substantial effects on estimating β-diversity but less on α-diversity. A high variation was also observed in the control across different samples (for example, 66.7-fold for the forward primer), suggesting that the amplicon sequencing-based detection approach could not be quantitative. In addition, various strategies were examined to improve the comparability of amplicon sequencing data, such as increasing biological replicates, and removing singleton sequences and less-representative OTUs across biological replicates. Finally, as expected, various statistical analyses with preprocessed experimental data revealed clear differences in the composition and structure of microbial communities between warming and non-warming, or between clipping and non-clipping. Taken together, these results suggest that amplicon sequencing-based detection is useful in analyzing microbial community structure even though it is not reproducible and quantitative. However, great caution should be taken in experimental design and data interpretation when the amplicon sequencing-based detection approach is used for quantitative analysis of the β-diversity of microbial communities. PMID:21346791

  4. PVWatts Version 1 Technical Reference

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dobos, A. P.

    2013-10-01

    The NREL PVWatts(TM) calculator is a web application developed by the National Renewable Energy Laboratory (NREL) that estimates the electricity production of a grid-connected photovoltaic system based on a few simple inputs. PVWatts combines a number of sub-models to predict overall system performance, and makes several hidden assumptions about performance parameters. This technical reference details the individual sub-models, documents assumptions and hidden parameters, and explains the sequence of calculations that yield the final system performance estimation.

  5. Final Technical Report for subcontract number B612144

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mayali, X.; Marcu, O.

    The original statement of work stipulated that the Subcontractor shall perform bacterial and algal cultivation and manipulation, microbe isolation, preparation of samples for sequencing and isotopic analysis, data analysis, and manuscript preparation. The Subcontractor shall work closely with Dr. Mayali and other LLNL scientists, and shall participate in monthly SFA meetings (either in person or by telephone). The Subcontractor shall deliver a final report at the conclusion of the work.

  6. It’s More Than Stamp Collecting: How Genome Sequencing Can Unify Biological Research

    PubMed Central

    Richards, Stephen

    2015-01-01

    The availability of reference genome sequences, especially the human reference, has revolutionized the study of biology. However, whilst the genomes of some species have been fully sequenced, a wide range of biological problems still cannot be effectively studied for lack of genome sequence information. Here, I identify neglected areas of biology and describe how both targeted species sequencing and more broad taxonomic surveys of the tree of life can address important biological questions. I enumerate the significant benefits that would accrue from sequencing a broader range of taxa, as well as discuss the technical advances in sequencing and assembly methods that would allow for wide-ranging application of whole-genome analysis. Finally, I suggest that in addition to “Big Science” survey initiatives to sequence the tree of life, a modified infrastructure-funding paradigm would better support reference genome sequence generation for research communities most in need. PMID:26003218

  7. It's more than stamp collecting: how genome sequencing can unify biological research.

    PubMed

    Richards, Stephen

    2015-07-01

    The availability of reference genome sequences, especially the human reference, has revolutionized the study of biology. However, while the genomes of some species have been fully sequenced, a wide range of biological problems still cannot be effectively studied for lack of genome sequence information. Here, I identify neglected areas of biology and describe how both targeted species sequencing and more broad taxonomic surveys of the tree of life can address important biological questions. I enumerate the significant benefits that would accrue from sequencing a broader range of taxa, as well as discuss the technical advances in sequencing and assembly methods that would allow for wide-ranging application of whole-genome analysis. Finally, I suggest that in addition to 'big science' survey initiatives to sequence the tree of life, a modified infrastructure-funding paradigm would better support reference genome sequence generation for research communities most in need. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. The human genome: Some assembly required. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1994-12-31

    The Human Genome Project promises to be one of the most rewarding endeavors in modern biology. The cost and the ethical and social implications, however, have made this project the source of considerable debate both in the scientific community and in the public at large. The 1994 Graduate Student Symposium addresses the scientific merits of the project, the technical issues involved in accomplishing the task, as well as the medical and social issues which stem from the wealth of knowledge which the Human Genome Project will help create. To this end, speakers were brought together who represent the diverse areasmore » of expertise characteristic of this multidisciplinary project. The keynote speaker addresses the project`s motivations and goals in the larger context of biological and medical sciences. The first two sessions address relevant technical issues, data collection with a focus on high-throughput sequencing methods and data analysis with an emphasis on identification of coding sequences. The third session explores recent advances in the understanding of genetic diseases and possible routes to treatment. Finally, the last session addresses some of the ethical, social and legal issues which will undoubtedly arise from having a detailed knowledge of the human genome.« less

  9. Protein Science by DNA Sequencing: How Advances in Molecular Biology Are Accelerating Biochemistry.

    PubMed

    Higgins, Sean A; Savage, David F

    2018-01-09

    A fundamental goal of protein biochemistry is to determine the sequence-function relationship, but the vastness of sequence space makes comprehensive evaluation of this landscape difficult. However, advances in DNA synthesis and sequencing now allow researchers to assess the functional impact of every single mutation in many proteins, but challenges remain in library construction and the development of general assays applicable to a diverse range of protein functions. This Perspective briefly outlines the technical innovations in DNA manipulation that allow massively parallel protein biochemistry and then summarizes the methods currently available for library construction and the functional assays of protein variants. Areas in need of future innovation are highlighted with a particular focus on assay development and the use of computational analysis with machine learning to effectively traverse the sequence-function landscape. Finally, applications in the fundamentals of protein biochemistry, disease prediction, and protein engineering are presented.

  10. ATD-1 Operational Integration Assessment Final Report

    NASA Technical Reports Server (NTRS)

    Witzberger, Kevin E.; Sharma, Shivanjli; Martin, Lynn Hazel; Wynnyk, Mitch; McGarry, Katie

    2015-01-01

    The FAA and NASA conducted an Operational Integration Assessment (OIA) of a prototype Terminal Sequencing and Spacing (formerly TSS, now TSAS) system at the FAA's William J. Hughes Technical Center (WJHTC). The OIA took approximately one year to plan and execute, culminating in a formal data collection, referred to as the Run for Record, from May 12-21, 2015. This report presents quantitative and qualitative results from the Run for Record.

  11. Missing data and technical variability in single-cell RNA-sequencing experiments.

    PubMed

    Hicks, Stephanie C; Townes, F William; Teng, Mingxiang; Irizarry, Rafael A

    2017-11-06

    Until recently, high-throughput gene expression technology, such as RNA-Sequencing (RNA-seq) required hundreds of thousands of cells to produce reliable measurements. Recent technical advances permit genome-wide gene expression measurement at the single-cell level. Single-cell RNA-Seq (scRNA-seq) is the most widely used and numerous publications are based on data produced with this technology. However, RNA-seq and scRNA-seq data are markedly different. In particular, unlike RNA-seq, the majority of reported expression levels in scRNA-seq are zeros, which could be either biologically-driven, genes not expressing RNA at the time of measurement, or technically-driven, genes expressing RNA, but not at a sufficient level to be detected by sequencing technology. Another difference is that the proportion of genes reporting the expression level to be zero varies substantially across single cells compared to RNA-seq samples. However, it remains unclear to what extent this cell-to-cell variation is being driven by technical rather than biological variation. Furthermore, while systematic errors, including batch effects, have been widely reported as a major challenge in high-throughput technologies, these issues have received minimal attention in published studies based on scRNA-seq technology. Here, we use an assessment experiment to examine data from published studies and demonstrate that systematic errors can explain a substantial percentage of observed cell-to-cell expression variability. Specifically, we present evidence that some of these reported zeros are driven by technical variation by demonstrating that scRNA-seq produces more zeros than expected and that this bias is greater for lower expressed genes. In addition, this missing data problem is exacerbated by the fact that this technical variation varies cell-to-cell. Then, we show how this technical cell-to-cell variability can be confused with novel biological results. Finally, we demonstrate and discuss how batch-effects and confounded experiments can intensify the problem. © The Author 2017. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  12. Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform

    PubMed Central

    Van Nostrand, Joy D.; Ning, Daliang; Sun, Bo; Xue, Kai; Liu, Feifei; Deng, Ye; Liang, Yuting; Zhou, Jizhong

    2017-01-01

    Illumina’s MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist. To assess reproducibility, 16S rRNA gene amplicons from 18 soil samples of a reciprocal transplantation experiment were sequenced on an Illumina MiSeq. The V4 region of 16S rRNA gene from each sample was sequenced in triplicate with each replicate having a unique barcode. The average OTU overlap, without considering sequence abundance, at a rarefaction level of 10,323 sequences was 33.4±2.1% and 20.2±1.7% between two and among three technical replicates, respectively. When OTU sequence abundance was considered, the average sequence abundance weighted OTU overlap was 85.6±1.6% and 81.2±2.1% for two and three replicates, respectively. Removing singletons significantly increased the overlap for both (~1–3%, p<0.001). Increasing the sequencing depth to 160,000 reads by deep sequencing increased OTU overlap both when sequence abundance was considered (95%) and when not (44%). However, if singletons were not removed the overlap between two technical replicates (not considering sequence abundance) plateaus at 39% with 30,000 sequences. Diversity measures were not affected by the low overlap as α-diversities were similar among technical replicates while β-diversities (Bray-Curtis) were much smaller among technical replicates than among treatment replicates (e.g., 0.269 vs. 0.374). Higher diversity coverage, but lower OTU overlap, was observed when replicates were sequenced in separate runs. Detrended correspondence analysis indicated that while there was considerable variation among technical replicates, the reproducibility was sufficient for detecting treatment effects for the samples examined. These results suggest that although there is variation among technical replicates, amplicon sequencing on MiSeq is useful for analyzing microbial community structure if used appropriately and with caution. For example, including technical replicates, removing spurious sequences and unrepresentative OTUs, using a clustering method with a high stringency for OTU generation, estimating treatment effects at higher taxonomic levels, and adapting the unique molecular identifier (UMI) and other newly developed methods to lower PCR and sequencing error and to identify true low abundance rare species all can increase reproducibility. PMID:28453559

  13. Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wen, Chongqing; Wu, Liyou; Qin, Yujia

    Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist. To assess reproducibility, 16S rRNA gene amplicons from 18 soil samples of a reciprocal transplantation experiment were sequenced on an Illumina MiSeq. The V4 region of 16S rRNA gene from each sample was sequenced in triplicate with each replicate having a unique barcode. The average OTU overlap, without considering sequence abundance, at a rarefaction level of 10,323 sequences was 33.4±2.1% and 20.2±1.7% between two and among three technical replicates, respectively. When OTU sequence abundance was considered,more » the average sequence abundance weighted OTU overlap was 85.6±1.6% and 81.2±2.1% for two and three replicates, respectively. Removing singletons significantly increased the overlap for both (~1-3%, p<0.001). Increasing the sequencing depth to 160,000 reads by deep sequencing increased OTU overlap both when sequence abundance was considered (95%) and when not (44%). However, if singletons were not removed the overlap between two technical replicates (not considering sequence abundance) plateaus at 39% with 30,000 sequences. Diversity measures were not affected by the low overlap as α-diversities were similar among technical replicates while β-diversities (Bray-Curtis) were much smaller among technical replicates than among treatment replicates (e.g., 0.269 vs. 0.374). Higher diversity coverage, but lower OTU overlap, was observed when replicates were sequenced in separate runs. Detrended correspondence analysis indicated that while there was considerable variation among technical replicates, the reproducibility was sufficient for detecting treatment effects for the samples examined. These results suggest that although there is variation among technical replicates, amplicon sequencing on MiSeq is useful for analyzing microbial community structure if used appropriately and with caution. For example, including technical replicates, removing spurious sequences and unrepresentative OTUs, using a clustering method with a high stringency for OTU generation, estimating treatment effects at higher taxonomic levels, and adapting the unique molecular identifier (UMI) and other newly developed methods to lower PCR and sequencing error and to identify true low abundance rare species all can increase reproducibility.« less

  14. Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform

    DOE PAGES

    Wen, Chongqing; Wu, Liyou; Qin, Yujia; ...

    2017-04-28

    Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist. To assess reproducibility, 16S rRNA gene amplicons from 18 soil samples of a reciprocal transplantation experiment were sequenced on an Illumina MiSeq. The V4 region of 16S rRNA gene from each sample was sequenced in triplicate with each replicate having a unique barcode. The average OTU overlap, without considering sequence abundance, at a rarefaction level of 10,323 sequences was 33.4±2.1% and 20.2±1.7% between two and among three technical replicates, respectively. When OTU sequence abundance was considered,more » the average sequence abundance weighted OTU overlap was 85.6±1.6% and 81.2±2.1% for two and three replicates, respectively. Removing singletons significantly increased the overlap for both (~1-3%, p<0.001). Increasing the sequencing depth to 160,000 reads by deep sequencing increased OTU overlap both when sequence abundance was considered (95%) and when not (44%). However, if singletons were not removed the overlap between two technical replicates (not considering sequence abundance) plateaus at 39% with 30,000 sequences. Diversity measures were not affected by the low overlap as α-diversities were similar among technical replicates while β-diversities (Bray-Curtis) were much smaller among technical replicates than among treatment replicates (e.g., 0.269 vs. 0.374). Higher diversity coverage, but lower OTU overlap, was observed when replicates were sequenced in separate runs. Detrended correspondence analysis indicated that while there was considerable variation among technical replicates, the reproducibility was sufficient for detecting treatment effects for the samples examined. These results suggest that although there is variation among technical replicates, amplicon sequencing on MiSeq is useful for analyzing microbial community structure if used appropriately and with caution. For example, including technical replicates, removing spurious sequences and unrepresentative OTUs, using a clustering method with a high stringency for OTU generation, estimating treatment effects at higher taxonomic levels, and adapting the unique molecular identifier (UMI) and other newly developed methods to lower PCR and sequencing error and to identify true low abundance rare species all can increase reproducibility.« less

  15. Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform.

    PubMed

    Wen, Chongqing; Wu, Liyou; Qin, Yujia; Van Nostrand, Joy D; Ning, Daliang; Sun, Bo; Xue, Kai; Liu, Feifei; Deng, Ye; Liang, Yuting; Zhou, Jizhong

    2017-01-01

    Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist. To assess reproducibility, 16S rRNA gene amplicons from 18 soil samples of a reciprocal transplantation experiment were sequenced on an Illumina MiSeq. The V4 region of 16S rRNA gene from each sample was sequenced in triplicate with each replicate having a unique barcode. The average OTU overlap, without considering sequence abundance, at a rarefaction level of 10,323 sequences was 33.4±2.1% and 20.2±1.7% between two and among three technical replicates, respectively. When OTU sequence abundance was considered, the average sequence abundance weighted OTU overlap was 85.6±1.6% and 81.2±2.1% for two and three replicates, respectively. Removing singletons significantly increased the overlap for both (~1-3%, p<0.001). Increasing the sequencing depth to 160,000 reads by deep sequencing increased OTU overlap both when sequence abundance was considered (95%) and when not (44%). However, if singletons were not removed the overlap between two technical replicates (not considering sequence abundance) plateaus at 39% with 30,000 sequences. Diversity measures were not affected by the low overlap as α-diversities were similar among technical replicates while β-diversities (Bray-Curtis) were much smaller among technical replicates than among treatment replicates (e.g., 0.269 vs. 0.374). Higher diversity coverage, but lower OTU overlap, was observed when replicates were sequenced in separate runs. Detrended correspondence analysis indicated that while there was considerable variation among technical replicates, the reproducibility was sufficient for detecting treatment effects for the samples examined. These results suggest that although there is variation among technical replicates, amplicon sequencing on MiSeq is useful for analyzing microbial community structure if used appropriately and with caution. For example, including technical replicates, removing spurious sequences and unrepresentative OTUs, using a clustering method with a high stringency for OTU generation, estimating treatment effects at higher taxonomic levels, and adapting the unique molecular identifier (UMI) and other newly developed methods to lower PCR and sequencing error and to identify true low abundance rare species all can increase reproducibility.

  16. Multiparametric prostate MRI: technical conduct, standardized report and clinical use.

    PubMed

    Manfredi, Matteo; Mele, Fabrizio; Garrou, Diletta; Walz, Jochen; Fütterer, Jurgen J; Russo, Filippo; Vassallo, Lorenzo; Villers, Arnauld; Emberton, Mark; Valerio, Massimo

    2018-02-01

    Multiparametric prostate MRI (mp-MRI) is an emerging imaging modality for diagnosis, characterization, staging, and treatment planning of prostate cancer (PCa). The technique, results reporting, and its role in clinical practice have been the subject of significant development over the last decade. Although mp-MRI is not yet routinely used in the diagnostic pathway, almost all urological guidelines have emphasized the potential role of mp-MRI in several aspects of PCa management. Moreover, new MRI sequences and scanning techniques are currently under evaluation to improve the diagnostic accuracy of mp-MRI. This review presents an overview of mp-MRI, summarizing the technical applications, the standardized reporting systems used, and their current roles in various stages of PCa management. Finally, this critical review also reports the main limitations and future perspectives of the technique.

  17. SUGAR: graphical user interface-based data refiner for high-throughput DNA sequencing.

    PubMed

    Sato, Yukuto; Kojima, Kaname; Nariai, Naoki; Yamaguchi-Kabata, Yumi; Kawai, Yosuke; Takahashi, Mamoru; Mimori, Takahiro; Nagasaki, Masao

    2014-08-08

    Next-generation sequencers (NGSs) have become one of the main tools for current biology. To obtain useful insights from the NGS data, it is essential to control low-quality portions of the data affected by technical errors such as air bubbles in sequencing fluidics. We develop a software SUGAR (subtile-based GUI-assisted refiner) which can handle ultra-high-throughput data with user-friendly graphical user interface (GUI) and interactive analysis capability. The SUGAR generates high-resolution quality heatmaps of the flowcell, enabling users to find possible signals of technical errors during the sequencing. The sequencing data generated from the error-affected regions of a flowcell can be selectively removed by automated analysis or GUI-assisted operations implemented in the SUGAR. The automated data-cleaning function based on sequence read quality (Phred) scores was applied to a public whole human genome sequencing data and we proved the overall mapping quality was improved. The detailed data evaluation and cleaning enabled by SUGAR would reduce technical problems in sequence read mapping, improving subsequent variant analysis that require high-quality sequence data and mapping results. Therefore, the software will be especially useful to control the quality of variant calls to the low population cells, e.g., cancers, in a sample with technical errors of sequencing procedures.

  18. Nanowire-nanopore transistor sensor for DNA detection during translocation

    NASA Astrophysics Data System (ADS)

    Xie, Ping; Xiong, Qihua; Fang, Ying; Qing, Quan; Lieber, Charles

    2011-03-01

    Nanopore sequencing, as a promising low cost, high throughput sequencing technique, has been proposed more than a decade ago. Due to the incompatibility between small ionic current signal and fast translocation speed and the technical difficulties on large scale integration of nanopore for direct ionic current sequencing, alternative methods rely on integrated DNA sensors have been proposed, such as using capacitive coupling or tunnelling current etc. But none of them have been experimentally demonstrated yet. Here we show that for the first time an amplified sensor signal has been experimentally recorded from a nanowire-nanopore field effect transistor sensor during DNA translocation. Independent multi-channel recording was also demonstrated for the first time. Our results suggest that the signal is from highly localized potential change caused by DNA translocation in none-balanced buffer condition. Given this method may produce larger signal for smaller nanopores, we hope our experiment can be a starting point for a new generation of nanopore sequencing devices with larger signal, higher bandwidth and large-scale multiplexing capability and finally realize the ultimate goal of low cost high throughput sequencing.

  19. Review of dynamic contrast-enhanced MRI: Technical aspects and applications in the musculoskeletal system.

    PubMed

    Sujlana, Parvinder; Skrok, Jan; Fayad, Laura M

    2018-04-01

    Although postcontrast imaging has been used for many years in musculoskeletal imaging, dynamic contrast enhanced (DCE) MRI is not routinely used in many centers around the world. Unlike conventional contrast-enhanced sequences, DCE-MRI allows the evaluation of the temporal pattern of enhancement in the musculoskeletal system, perhaps best known for its use in oncologic applications (such as differentiating benign from malignant tumors, evaluating for treatment response after neoadjuvant chemotherapy, and differentiating postsurgical changes from residual tumor). However, DCE-MRI can also be used to evaluate inflammatory processes such as Charcot foot and synovitis, and evaluate bone perfusion in entities like Legg Calve Perthes disease and arthritis. Finally, vascular abnormalities and associated complications may be better characterized with DCE-MRI than conventional imaging. The goal of this article is to review the applications and technical aspects of DCE-MRI in the musculoskeletal system. 5 Technical Efficacy: Stage 3 J. Magn. Reson. Imaging 2018;47:875-890. © 2017 International Society for Magnetic Resonance in Medicine.

  20. Final Technical Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Glasser, Alan H.

    Final technical report on DE-SC0016106. This is the final technical report for a portion of the multi-institutional CEMM project. This report is centered around 3 publications and a seminar presentation, which have been submitted to E-Link.

  1. 10 CFR 52.157 - Contents of applications; technical information in final safety analysis report.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...; technical information in final safety analysis report. The application must contain a final safety analysis...) Information sufficient to demonstrate compliance with the applicable requirements regarding testing, analysis... 10 Energy 2 2013-01-01 2013-01-01 false Contents of applications; technical information in final...

  2. 10 CFR 52.157 - Contents of applications; technical information in final safety analysis report.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...; technical information in final safety analysis report. The application must contain a final safety analysis...) Information sufficient to demonstrate compliance with the applicable requirements regarding testing, analysis... 10 Energy 2 2012-01-01 2012-01-01 false Contents of applications; technical information in final...

  3. 10 CFR 52.157 - Contents of applications; technical information in final safety analysis report.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...; technical information in final safety analysis report. The application must contain a final safety analysis...) Information sufficient to demonstrate compliance with the applicable requirements regarding testing, analysis... 10 Energy 2 2014-01-01 2014-01-01 false Contents of applications; technical information in final...

  4. 10 CFR 52.157 - Contents of applications; technical information in final safety analysis report.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...; technical information in final safety analysis report. The application must contain a final safety analysis...) Information sufficient to demonstrate compliance with the applicable requirements regarding testing, analysis... 10 Energy 2 2011-01-01 2011-01-01 false Contents of applications; technical information in final...

  5. RAS testing in metastatic colorectal cancer: advances in Europe.

    PubMed

    Van Krieken, J Han J M; Rouleau, Etienne; Ligtenberg, Marjolijn J L; Normanno, Nicola; Patterson, Scott D; Jung, Andreas

    2016-04-01

    Personalized medicine shows promise for maximizing efficacy and minimizing toxicity of anti-cancer treatment. KRAS exon 2 mutations are predictive of resistance to epidermal growth factor receptor-directed monoclonal antibodies in patients with metastatic colorectal cancer. Recent studies have shown that broader RAS testing (KRAS and NRAS) is needed to select patients for treatment. While Sanger sequencing is still used, approaches based on various methodologies are available. Few CE-approved kits, however, detect the full spectrum of RAS mutations. More recently, "next-generation" sequencing has been developed for research use, including parallel semiconductor sequencing and reversible termination. These techniques have high technical sensitivities for detecting mutations, although the ideal threshold is currently unknown. Finally, liquid biopsy has the potential to become an additional tool to assess tumor-derived DNA. For accurate and timely RAS testing, appropriate sampling and prompt delivery of material is critical. Processes to ensure efficient turnaround from sample request to RAS evaluation must be implemented so that patients receive the most appropriate treatment. Given the variety of methodologies, external quality assurance programs are important to ensure a high standard of RAS testing. Here, we review technical and practical aspects of RAS testing for pathologists working with metastatic colorectal cancer tumor samples. The extension of markers from KRAS to RAS testing is the new paradigm for biomarker testing in colorectal cancer.

  6. PVWatts Version 5 Manual

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dobos, A. P.

    2014-09-01

    The NREL PVWatts calculator is a web application developed by the National Renewable Energy Laboratory (NREL) that estimates the electricity production of a grid-connected photovoltaic system based on a few simple inputs. PVWatts combines a number of sub-models to predict overall system performance, and makes includes several built-in parameters that are hidden from the user. This technical reference describes the sub-models, documents assumptions and hidden parameters, and explains the sequence of calculations that yield the final system performance estimate. This reference is applicable to the significantly revised version of PVWatts released by NREL in 2014.

  7. Launch Vehicle Design Process: Characterization, Technical Integration, and Lessons Learned

    NASA Technical Reports Server (NTRS)

    Blair, J. C.; Ryan, R. S.; Schutzenhofer, L. A.; Humphries, W. R.

    2001-01-01

    Engineering design is a challenging activity for any product. Since launch vehicles are highly complex and interconnected and have extreme energy densities, their design represents a challenge of the highest order. The purpose of this document is to delineate and clarify the design process associated with the launch vehicle for space flight transportation. The goal is to define and characterize a baseline for the space transportation design process. This baseline can be used as a basis for improving effectiveness and efficiency of the design process. The baseline characterization is achieved via compartmentalization and technical integration of subsystems, design functions, and discipline functions. First, a global design process overview is provided in order to show responsibility, interactions, and connectivity of overall aspects of the design process. Then design essentials are delineated in order to emphasize necessary features of the design process that are sometimes overlooked. Finally the design process characterization is presented. This is accomplished by considering project technical framework, technical integration, process description (technical integration model, subsystem tree, design/discipline planes, decision gates, and tasks), and the design sequence. Also included in the document are a snapshot relating to process improvements, illustrations of the process, a survey of recommendations from experienced practitioners in aerospace, lessons learned, references, and a bibliography.

  8. Standardization and quality management in next-generation sequencing.

    PubMed

    Endrullat, Christoph; Glökler, Jörn; Franke, Philipp; Frohme, Marcus

    2016-09-01

    DNA sequencing continues to evolve quickly even after > 30 years. Many new platforms suddenly appeared and former established systems have vanished in almost the same manner. Since establishment of next-generation sequencing devices, this progress gains momentum due to the continually growing demand for higher throughput, lower costs and better quality of data. In consequence of this rapid development, standardized procedures and data formats as well as comprehensive quality management considerations are still scarce. Here, we listed and summarized current standardization efforts and quality management initiatives from companies, organizations and societies in form of published studies and ongoing projects. These comprise on the one hand quality documentation issues like technical notes, accreditation checklists and guidelines for validation of sequencing workflows. On the other hand, general standard proposals and quality metrics are developed and applied to the sequencing workflow steps with the main focus on upstream processes. Finally, certain standard developments for downstream pipeline data handling, processing and storage are discussed in brief. These standardization approaches represent a first basis for continuing work in order to prospectively implement next-generation sequencing in important areas such as clinical diagnostics, where reliable results and fast processing is crucial. Additionally, these efforts will exert a decisive influence on traceability and reproducibility of sequence data.

  9. Otoplasty: sequencing the operation for improved results.

    PubMed

    Hoehn, James G; Ashruf, Salman

    2005-01-01

    : After studying this article, the participant should be able to: 1. Understand the anatomy and embryology of the external ear. 2. Understand the anatomic causes of the prominent ear. 3. Understand the operative maneuvers used to shape the external ear. 4. Be able to sequence the otoplasty for consistent results. 5. Understand the possible complications of the otoplasty procedure. Correction of prominent ears is a common plastic surgical procedure. Proper execution of the surgical techniques is dependent on the surgeon's understanding of the surgical procedure. This understanding is best founded on an understanding of the historical bases for the operative steps and the execution of these operative steps in a logical fashion. This article describes the concept of sequencing the operation of otoplasty to produce predictable results combining the technical contributions from many authors. The historical, embryological, and anatomic bases for the operation are also discussed. Finally, the authors' preferred techniques are presented. Sequencing the steps in the preoperative assessment, preoperative planning, patient management, operative technique, and postoperative care will produce reproducible results for the attentive surgeon. Careful attention to the details of the operation of otoplasty will avoid many postoperative problems.

  10. Henry Taube and Coordination Chemistry

    Science.gov Websites

    Shifts Caused by Cr++ in Aqueous Solutions, DOE Technical Report, 1962 Reactions of Solvated Ions Final Report, DOE Technical Report, 1962 Isotopic Discrimination of Some Solutes in Liquid Ammonia, DOE Technical Report, 1966 Final Technical Report of Research, DOE Technical Report, 1972 Top Additional Web

  11. George A. Olah, Carbocation and Hydrocarbon Chemistry

    Science.gov Websites

    . Final Technical Report. [HF:BF{sub 2}/H{sub 2}] , DOE Technical Report, 1980 Superacid Catalyzed Coal Conversion Chemistry. 1st and 2nd Quarterly Technical Progress Reports, September 1, 1983-March 30, 1984 , DOE Technical Report, 1984 Superacid Catalyzed Coal Conversion Chemistry. Final Technical Report

  12. 77 FR 46306 - Fluxapyroxad; Pesticide Tolerances Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-03

    ...; Pesticide Tolerances Technical Amendment AGENCY: Environmental Protection Agency (EPA). ACTION: Final rule; technical amendment. SUMMARY: EPA issued a final rule in the Federal Register of May 14, 2012, concerning.... Inadvertently, the terminology for the oilseed crop group and for dried plums was incorrect. This technical...

  13. RNase H-assisted RNA-primed rolling circle amplification for targeted RNA sequence detection.

    PubMed

    Takahashi, Hirokazu; Ohkawachi, Masahiko; Horio, Kyohei; Kobori, Toshiro; Aki, Tsunehiro; Matsumura, Yukihiko; Nakashimada, Yutaka; Okamura, Yoshiko

    2018-05-17

    RNA-primed rolling circle amplification (RPRCA) is a useful laboratory method for RNA detection; however, the detection of RNA is limited by the lack of information on 3'-terminal sequences. We uncovered that conventional RPRCA using pre-circularized probes could potentially detect the internal sequence of target RNA molecules in combination with RNase H. However, the specificity for mRNA detection was low, presumably due to non-specific hybridization of non-target RNA with the circular probe. To overcome this technical problem, we developed a method for detecting a sequence of interest in target RNA molecules via RNase H-assisted RPRCA using padlocked probes. When padlock probes are hybridized to the target RNA molecule, they are converted to the circular form by SplintR ligase. Subsequently, RNase H creates nick sites only in the hybridized RNA sequence, and single-stranded DNA is finally synthesized from the nick site by phi29 DNA polymerase. This method could specifically detect at least 10 fmol of the target RNA molecule without reverse transcription. Moreover, this method detected GFP mRNA present in 10 ng of total RNA isolated from Escherichia coli without background DNA amplification. Therefore, this method can potentially detect almost all types of RNA molecules without reverse transcription and reveal full-length sequence information.

  14. Automatic Keyword Extraction from Individual Documents

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rose, Stuart J.; Engel, David W.; Cramer, Nicholas O.

    2010-05-03

    This paper introduces a novel and domain-independent method for automatically extracting keywords, as sequences of one or more words, from individual documents. We describe the method’s configuration parameters and algorithm, and present an evaluation on a benchmark corpus of technical abstracts. We also present a method for generating lists of stop words for specific corpora and domains, and evaluate its ability to improve keyword extraction on the benchmark corpus. Finally, we apply our method of automatic keyword extraction to a corpus of news articles and define metrics for characterizing the exclusivity, essentiality, and generality of extracted keywords within a corpus.

  15. High-fidelity target sequencing of individual molecules identified using barcode sequences: de novo detection and absolute quantitation of mutations in plasma cell-free DNA from cancer patients.

    PubMed

    Kukita, Yoji; Matoba, Ryo; Uchida, Junji; Hamakawa, Takuya; Doki, Yuichiro; Imamura, Fumio; Kato, Kikuya

    2015-08-01

    Circulating tumour DNA (ctDNA) is an emerging field of cancer research. However, current ctDNA analysis is usually restricted to one or a few mutation sites due to technical limitations. In the case of massively parallel DNA sequencers, the number of false positives caused by a high read error rate is a major problem. In addition, the final sequence reads do not represent the original DNA population due to the global amplification step during the template preparation. We established a high-fidelity target sequencing system of individual molecules identified in plasma cell-free DNA using barcode sequences; this system consists of the following two steps. (i) A novel target sequencing method that adds barcode sequences by adaptor ligation. This method uses linear amplification to eliminate the errors introduced during the early cycles of polymerase chain reaction. (ii) The monitoring and removal of erroneous barcode tags. This process involves the identification of individual molecules that have been sequenced and for which the number of mutations have been absolute quantitated. Using plasma cell-free DNA from patients with gastric or lung cancer, we demonstrated that the system achieved near complete elimination of false positives and enabled de novo detection and absolute quantitation of mutations in plasma cell-free DNA. © The Author 2015. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

  16. 32 CFR 37.895 - How is the final performance report to be sent to the Defense Technical Information Center?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... How is the final performance report to be sent to the Defense Technical Information Center? (a... 32 National Defense 1 2014-07-01 2014-07-01 false How is the final performance report to be sent to the Defense Technical Information Center? 37.895 Section 37.895 National Defense Department of...

  17. 32 CFR 37.895 - How is the final performance report to be sent to the Defense Technical Information Center?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... How is the final performance report to be sent to the Defense Technical Information Center? (a... 32 National Defense 1 2011-07-01 2011-07-01 false How is the final performance report to be sent to the Defense Technical Information Center? 37.895 Section 37.895 National Defense Department of...

  18. 32 CFR 37.895 - How is the final performance report to be sent to the Defense Technical Information Center?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... How is the final performance report to be sent to the Defense Technical Information Center? (a... 32 National Defense 1 2013-07-01 2013-07-01 false How is the final performance report to be sent to the Defense Technical Information Center? 37.895 Section 37.895 National Defense Department of...

  19. 32 CFR 37.895 - How is the final performance report to be sent to the Defense Technical Information Center?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 1 2012-07-01 2012-07-01 false How is the final performance report to be sent to the Defense Technical Information Center? 37.895 Section 37.895 National Defense Department of... How is the final performance report to be sent to the Defense Technical Information Center? (a...

  20. Technical-economical analysis of selected decentralized technologies for municipal wastewater treatment in the city of Rome.

    PubMed

    Gavasci, Renato; Chiavola, Agostina; Spizzirri, Massimo

    2010-01-01

    Several wastewater treatment technologies were evaluated as alternative systems to the more traditional centralized continuous flow system to serve decentralized areas of the city of Rome (Italy). For instance, the following technologies were selected: (1) Constructed wetlands, (2) Membrane Biological Reactor, (3) Deep Shaft, (4) Sequencing Batch Reactor, and (5) Combined Filtration and UV-disinfection. Such systems were distinguished based on the limits they are potentially capable of accomplishing on the effluent. Consequently, the SBR and DS were grouped together for their capability to comply with the standards for the discharge into surface waters (according to the Italian D.Lgs. 152/06, Table 1, All. 5), whereas the MBR and tertiary system (Filtration+UVc-disinfection) were considered together as they should be able to allow effluent discharge into soil (according to the Italian D.Lgs. 152/06, Table 4, All. 5) and/or reuse (according to the Italian D.M. 185/03). Both groups of technologies were evaluated in comparison with the more common continuous flow treatment sequence consisting of a biological activated sludge tank followed by the secondary settlement, with final chlorination. CWs were studied separately as a solution for decentralized urban areas with limited population. After the analysis of the main technical features, an economical estimate was carried out taking into account the investment, operation and maintenance costs as a function of the plant's capacity. The analysis was based on real data provided by the Company who manages the entire water system of the City of Rome (Acea Ato 2 S.p.A.). A preliminary design of the treatment plants using some of the selected technologies was finally carried out.

  1. Single-Cell Genomic Analysis in Plants

    PubMed Central

    Hu, Haifei; Scheben, Armin; Edwards, David

    2018-01-01

    Individual cells in an organism are variable, which strongly impacts cellular processes. Advances in sequencing technologies have enabled single-cell genomic analysis to become widespread, addressing shortcomings of analyses conducted on populations of bulk cells. While the field of single-cell plant genomics is in its infancy, there is great potential to gain insights into cell lineage and functional cell types to help understand complex cellular interactions in plants. In this review, we discuss current approaches for single-cell plant genomic analysis, with a focus on single-cell isolation, DNA amplification, next-generation sequencing, and bioinformatics analysis. We outline the technical challenges of analysing material from a single plant cell, and then examine applications of single-cell genomics and the integration of this approach with genome editing. Finally, we indicate future directions we expect in the rapidly developing field of plant single-cell genomic analysis. PMID:29361790

  2. 76 FR 18624 - Research, Technical Assistance and Training Programs: Notice of Final Circular

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-04

    ... to FTA Circular 6100.1D, Research and Technical Assistance Training Program: Application Instructions... DEPARTMENT OF TRANSPORTATION Federal Transit Administration Research, Technical Assistance and Training Programs: Notice of Final Circular AGENCY: Federal Transit Administration (FTA), DOT. ACTION...

  3. Minnesota Deaf-Blind Technical Assistance Project. Final Report.

    ERIC Educational Resources Information Center

    Kloos, Eric

    This final report describes activities and accomplishments of the 3-year federally supported Minnesota Deaf-Blind Technical Assistance Project. The project provided training and technical assistance, information sharing, and support services to families of children with deaf-blindness. Activities and accomplishments included: collaboration with…

  4. 75 FR 56857 - Pilot, Flight Instructor, and Pilot School Certification

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-17

    ... of part 141. Discussion of Technical Amendment Section 141.5(d) establishes the quality of training... Certification AGENCY: Federal Aviation Administration, DOT. ACTION: Final rule; technical amendment. SUMMARY: The Federal Aviation Administration (FAA) is making minor technical changes to a final rule published...

  5. 77 FR 29247 - Federal Motor Vehicle Safety Standards; Occupant Crash Protection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-17

    ...). ACTION: Final rule; technical amendments. SUMMARY: This final rule makes technical amendments to Federal... advanced air bag requirements. As written now, the general warning label requirements contain an explicit... equipment requirements for restraint systems. This document makes technical amendments to several of the...

  6. HIV-1 tropism testing in subjects achieving undetectable HIV-1 RNA: diagnostic accuracy, viral evolution and compartmentalization.

    PubMed

    Pou, Christian; Codoñer, Francisco M; Thielen, Alexander; Bellido, Rocío; Pérez-Álvarez, Susana; Cabrera, Cecilia; Dalmau, Judith; Curriu, Marta; Lie, Yolanda; Noguera-Julian, Marc; Puig, Jordi; Martínez-Picado, Javier; Blanco, Julià; Coakley, Eoin; Däumer, Martin; Clotet, Bonaventura; Paredes, Roger

    2013-01-01

    Technically, HIV-1 tropism can be evaluated in plasma or peripheral blood mononuclear cells (PBMCs). However, only tropism testing of plasma HIV-1 has been validated as a tool to predict virological response to CCR5 antagonists in clinical trials. The preferable tropism testing strategy in subjects with undetectable HIV-1 viremia, in whom plasma tropism testing is not feasible, remains uncertain. We designed a proof-of-concept study including 30 chronically HIV-1-infected individuals who achieved HIV-1 RNA <50 copies/mL during at least 2 years after first-line ART initiation. First, we determined the diagnostic accuracy of 454 and population sequencing of gp120 V3-loops in plasma and PBMCs, as well as of MT-2 assays before ART initiation. The Enhanced Sensitivity Trofile Assay (ESTA) was used as the technical reference standard. 454 sequencing of plasma viruses provided the highest agreement with ESTA. The accuracy of 454 sequencing decreased in PBMCs due to reduced specificity. Population sequencing in plasma and PBMCs was slightly less accurate than plasma 454 sequencing, being less sensitive but more specific. MT-2 assays had low sensitivity but 100% specificity. Then, we used optimized 454 sequence data to investigate viral evolution in PBMCs during viremia suppression and only found evolution of R5 viruses in one subject. No de novo CXCR4-using HIV-1 production was observed over time. Finally, Slatkin-Maddison tests suggested that plasma and cell-associated V3 forms were sometimes compartmentalized. The absence of tropism shifts during viremia suppression suggests that, when available, testing of stored plasma samples is generally safe and informative, provided that HIV-1 suppression is maintained. Tropism testing in PBMCs may not necessarily produce equivalent biological results to plasma, because the structure of viral populations and the diagnostic performance of tropism assays may sometimes vary between compartments. Thereby, proviral DNA tropism testing should be specifically validated in clinical trials before it can be applied to routine clinical decision-making.

  7. Conceptual design of a moving belt radiator shuttle-attached experiments: Technical requirement Document

    NASA Technical Reports Server (NTRS)

    Aguilar, Jerry L.

    1989-01-01

    The technical requirements for a shuttle-attached Moving Belt Radiator (MBR) experiment are defined. The MBR is an advanced radiator concept in which a rotating belt radiates thermal energy to space. The requirements for integrating the MBR experiment in the shuttle bay are discussed. Requirements for the belt material and working fluid are outlined along with some possible options. The proposed size and relationship to a full scale Moving Belt Radiator are defined. The experiment is defined with the primary goal of dynamic testing and a secondary goal of demonstrating the sealing and heat transfer characteristics. A perturbation system which will simulate a docking maneuver or other type of short term acceleration is proposed for inclusion in the experimental apparatus. A deployment and retraction capability which will aid in evaluating the dynamics of a belt during such a maneuver is also described. The proposed test sequence for the experiment is presented. Details of the conceptual design are not presented herein, but rather in a separate Final Report.

  8. DGs for Service Restoration to Critical Loads in a Secondary Network

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Xu, Yin; Liu, Chen-Ching; Wang, Zhiwen

    During a major outage in a secondary network distribution system, distributed generators (DGs) connected to the primary feeders as well as the secondary network can be used to serve critical loads. This paper proposed a resilience-oriented method to determine restoration strategies for secondary network distribution systems after a major disaster. Technical issues associated with the restoration process are analyzed, including the operation of network protectors, inrush currents caused by the energization of network transformers, synchronization of DGs to the network, and circulating currents among DGs. A look-ahead load restoration framework is proposed, incorporating technical issues associated with secondary networks, limitsmore » on DG capacity and generation resources, dynamic constraints, and operational limits. The entire outage duration is divided into a sequence of periods. Restoration strategies can be adjusted at the beginning of each period using the latest information. Finally, numerical simulation of the modified IEEE 342-node low voltage networked test system is performed to validate the effectiveness of the proposed method.« less

  9. DGs for Service Restoration to Critical Loads in a Secondary Network

    DOE PAGES

    Xu, Yin; Liu, Chen-Ching; Wang, Zhiwen; ...

    2017-08-25

    During a major outage in a secondary network distribution system, distributed generators (DGs) connected to the primary feeders as well as the secondary network can be used to serve critical loads. This paper proposed a resilience-oriented method to determine restoration strategies for secondary network distribution systems after a major disaster. Technical issues associated with the restoration process are analyzed, including the operation of network protectors, inrush currents caused by the energization of network transformers, synchronization of DGs to the network, and circulating currents among DGs. A look-ahead load restoration framework is proposed, incorporating technical issues associated with secondary networks, limitsmore » on DG capacity and generation resources, dynamic constraints, and operational limits. The entire outage duration is divided into a sequence of periods. Restoration strategies can be adjusted at the beginning of each period using the latest information. Finally, numerical simulation of the modified IEEE 342-node low voltage networked test system is performed to validate the effectiveness of the proposed method.« less

  10. 77 FR 30512 - Native American Career and Technical Education Program; Final Waivers and Extension of Project...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-23

    ... DEPARTMENT OF EDUCATION Native American Career and Technical Education Program; Final Waivers and... American Career and Technical Education Program Catalog of Federal Domestic Assistance (CFDA) Number: 84.101A. SUMMARY: For 60-month projects funded in fiscal year (FY) 2007 under the Native American Career...

  11. 48 CFR 1852.235-73 - Final Scientific and Technical Reports.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Final Scientific and Technical Reports. 1852.235-73 Section 1852.235-73 Federal Acquisition Regulations System NATIONAL..., including recommendations and conclusions based on the experience and results obtained. The final report...

  12. Coosa River Storage Annex, Talladega, Alabama Environmental Investigation, Final Management and Resources Utilization Plan

    DTIC Science & Technology

    1991-02-01

    to adequately assess the health and environmental risks associated with the closure and transfer of the Annex forI other use; and 3) identification of...1990); Draft Final Technical Plan, Draft Final Sampling Design Plan and Draft Final Health and Safety Plan, USATHAMA, June 1990. 2.1.2 Draft Final...Final Technical Plan, Sampling Design Plan and Health and Safety Plan) supplied by USATHAMA. The estimate may be revised, with USATHAMA approval, as

  13. Promises, pitfalls and practicalities of prenatal whole exome sequencing.

    PubMed

    Best, Sunayna; Wou, Karen; Vora, Neeta; Van der Veyver, Ignatia B; Wapner, Ronald; Chitty, Lyn S

    2018-01-01

    Prenatal genetic diagnosis provides information for pregnancy and perinatal decision-making and management. In several small series, prenatal whole exome sequencing (WES) approaches have identified genetic diagnoses when conventional tests (karyotype and microarray) were not diagnostic. Here, we review published prenatal WES studies and recent conference abstracts. Thirty-one studies were identified, with diagnostic rates in series of five or more fetuses varying between 6.2% and 80%. Differences in inclusion criteria and trio versus singleton approaches to sequencing largely account for the wide range of diagnostic rates. The data suggest that diagnostic yields will be greater in fetuses with multiple anomalies or in cases preselected following genetic review. Beyond its ability to improve diagnostic rates, we explore the potential of WES to improve understanding of prenatal presentations of genetic disorders and lethal fetal syndromes. We discuss prenatal phenotyping limitations, counselling challenges regarding variants of uncertain significance, incidental and secondary findings, and technical problems in WES. We review the practical, ethical, social and economic issues that must be considered before prenatal WES could become part of routine testing. Finally, we reflect upon the potential future of prenatal genetic diagnosis, including a move towards whole genome sequencing and non-invasive whole exome and whole genome testing. © 2017 John Wiley & Sons, Ltd. © 2017 John Wiley & Sons, Ltd.

  14. 77 FR 50589 - Agreements and Memoranda of Understanding Between the Food and Drug Administration and Other...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-22

    ...: Final rule. SUMMARY: This final rule makes technical changes that will update a requirement that many of the written agreements and memoranda of understanding (MOUs) between the Food and Drug Administration.... This final rule, accordingly, eliminates it. We are making these technical changes to conserve Agency...

  15. 77 FR 30514 - Native Hawaiian Career and Technical Education Program; Final Waiver and Extension of Project Period

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-23

    ... DEPARTMENT OF EDUCATION Native Hawaiian Career and Technical Education Program; Final Waiver and.... ACTION: Notice. Overview Information Final Waiver and Extension of Project Period for the Native Hawaiian.... SUMMARY: For 36-month projects funded in fiscal year (FY) 2009 under the Native Hawaiian Career and...

  16. Soldier communication net for the 21st century digitized battlespace

    NASA Astrophysics Data System (ADS)

    Mu, Libo; Zhang, Yutian

    1999-07-01

    This paper present soldier communication net scheme, which survives and operates in the 21st century battlefield environment. First, it analyzes the features, the need, function of the soldier communication net on the 21st century battlefield environment. Secondly it presents a layered model of the soldier communication net, derived from the OSI theory, and discusses the design of the 3 layers, link layer, link controller and input/output applications layer. Thirdly, it present some key technical discussion concerning with the direct-sequence-spread-spectrum communication, code/decode and low power consumption. Finally, it gives the conclusion that spread spectrum time division system is the best scheme of soldier communication net.

  17. A new peer instruction method for teaching practical skills in the health sciences: an evaluation of the 'Learning Trail'.

    PubMed

    Dollman, James

    2005-01-01

    The 'Learning Trail' is an innovative application of peer-mediated instruction designed to enhance student learning in large practical classes. The strategy specifically seeks to improve participants' attention to details of protocol that are often difficult to observe during teacher-centered demonstrations to large groups. Students (n=68) at the University of South Australia trialed this strategy, in which instruction in anthropometric techniques is initiated by an instructor to a group of 3-4 students and then sent in 'waves' from one student group to the next. The final group in the sequence demonstrates the techniques to the instructor, who notes any departures from technical accuracy. As each technical module is flowing from group to group, the instructor initiates the next 'wave' with the first group, and the process is repeated until all of the relevant skills are processed. The final stage is a full class discussion during which sources of technical error are identified and resolved. In this trial, students taught skinfold measurement by the peer instructed method (PI; n=33) were compared with a traditionally instructed group (TI; n=35), in which the instructor was responsible for all information transfer. For each participant, technical errors of measurement (TEM) were calculated; the intra-tester TEM as a measure of reliability, and the inter-tester TEM, in which the student's measures are compared with those of a criterion anthropometrist to give an indication of validity. There were no differences between TI and PI groups on intra-tester TEM (p=0.24), but the PI group had a lower inter-tester TEM for pooled skinfold sites (p=0.006) and for one individual site (triceps; p=0.007), but not the other three sites. The time taken to complete the whole set of instructions did not differ between delivery modes. The results of this trial suggest that the peer-mediated strategy may be more effective than teacher-centered instruction in terms of technical accuracy in anthropometry.

  18. 2005 v4.3 Technical Support Document

    EPA Pesticide Factsheets

    Emissions Modeling for the Final Mercury and Air Toxics Standards Technical Support Document describes how updated 2005 NEI, version 2 emissions were processed for air quality modeling in support of the final Mercury and Air Toxics Standards (MATS).

  19. The Center for Space Telemetering and Telecommunications Systems

    NASA Technical Reports Server (NTRS)

    Horan, S.; DeLeon, P.; Borah, D.; Lyman, R.

    2003-01-01

    This report comprises the final technical report for the research grant 'Center for Space Telemetering and Telecommunications Systems' sponsored by the National Aeronautics and Space Administration's Goddard Space Flight Center. The grant activities are broken down into the following technology areas: (1) Space Protocol Testing; (2) Autonomous Reconfiguration of Ground Station Receivers; (3) Satellite Cluster Communications; and (4) Bandwidth Efficient Modulation. The grant activity produced a number of technical reports and papers that were communicated to NASA as they were generated. This final report contains the final summary papers or final technical report conclusions for each of the project areas. Additionally, the grant supported students who made progress towards their degrees while working on the research.

  20. Promotion of the Equal Access of Girls and Women to Technical and Vocational Education. Studies on Technical and Vocational Education 7.

    ERIC Educational Resources Information Center

    United Nations Educational, Scientific, and Cultural Organization, Paris (France).

    This monograph includes the final report of the International Expert Meeting on the Promotion of Equal Access of Girls and Women to Technical and Vocational Education (TVE) held in Seoul, Republic of Korea, and country discussion papers. The final report is composed of an introduction that proposes that many Member States require special measures…

  1. Air Quality Modeling Technical Support Document for the Final Cross State Air Pollution Rule Update

    EPA Pesticide Factsheets

    In this technical support document (TSD) we describe the air quality modeling performed to support the final Cross State Air Pollution Rule for the 2008 ozone National Ambient Air Quality Standards (NAAQS).

  2. 7 CFR 614.7 - Preliminary technical determinations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Preliminary technical determinations. 614.7 Section... Preliminary technical determinations. (a) A preliminary technical determination becomes final 30 days after... purpose of gathering additional information and discussing the facts relating to the preliminary technical...

  3. Metallurgical Evaluations of Depainting Processes on Aluminum Substrate

    NASA Technical Reports Server (NTRS)

    McGill, Preston

    1999-01-01

    In December 1993, the Environmental Protection Agency (EPA) Emission Standards Division and the National Aeronautics and Space Administration's (NASA's) Marshall Space Flight Center (MSFC) signed an Interagency Agreement (IA) initiating a task force for the technical assessment of alternative technologies for aerospace depainting operations. The United States Air Force (USAF) joined the task force in 1994. The mandates of the task force were: (1) To identify available alternative depainting systems that do not rely on methylene chloride or other ozone-depleting, chlorinated, and volatile organic carbon solvents. (2) To determine the viability, applicability, and pollution prevention potential of each identified alternative. (3) To address issues of safety, environmental impact, reliability, and maintainability. Through a Technical Implementation Committee (TIC), the task force selected and evaluated eight alternative paint stripping technologies: chemical stripping, carbon dioxide (CO2) blasting, xenon flashlamp and CO2 coatings removal (FLASHJET(R)), CO2 laser stripping, plastic media blasting (PMB), sodium bicarbonate wet stripping, high-pressure water blasting (WaterJet), and wheat starch abrasive blasting (Enviro-Strip(R)). (The CO2 blasting study was discontinued after the first depainting sequence.) This final report presents the results of the Joint EPA/NASA/USAF Interagency Depainting Study. Significant topics include: (1) Final depainting sequence data for the chemical stripping, PMB, sodium bicarbonate wet stripping, and WaterJet processes. (2) Strip rates for all eight technologies. (3) Sequential comparisons of surface roughness measurements for the seven viable depainting technologies. (4) Chronological reviews of and lessons learned in the conduct of all eight technologies. (5) An analysis of the surface roughness trends for each of the seven technologies. (6) Metallurgic evaluations of panels Summaries of corrosion and hydrogen embrittlement evaluations of chemical stripping panels, detailed descriptions of which appear in previous reports. Because the requirements for alternative systems are diverse, as are initial setup, training, and on-going operational considerations, this study does not recommend a particular product or process. Users of this study will draw their own conclusions from the data presented herein.

  4. Programs of Study as a State Policy Mandate: A Longitudinal Study of the South Carolina Personal Pathways to Success Initiative. Final Technical Report: Major Findings and Implications

    ERIC Educational Resources Information Center

    Hammond, Cathy; Drew, Sam F.; Withington, Cairen; Griffith, Cathy; Swiger, Caroline M.; Mobley, Catherine; Sharp, Julia L.; Stringfield, Samuel C.; Stipanovic, Natalie; Daugherty, Lindsay

    2013-01-01

    This is the final technical report from the National Research Center for Career and Technical Education's (NRCCTE's) five-year longitudinal study of South Carolina's Personal Pathway to Success initiative, which was authorized by the state's Education and Economic Development Act (EEDA) in 2005. NRCCTE-affiliated researchers at the National…

  5. Direct Final Rule: Nonroad Diesel Technical Amendments and Tier 3 Technical Relief Provision

    EPA Pesticide Factsheets

    Rule making certain technical corrections to the rules establishing emission standards for nonroad diesel engines and amending those rules to provide manufacturers with a production technical relief provision for Tier 3 equipment.

  6. GAP Final Technical Report 12-14-04

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Andrew J. Bordner, PhD, Senior Research Scientist

    2004-12-14

    The Genomics Annotation Platform (GAP) was designed to develop new tools for high throughput functional annotation and characterization of protein sequences and structures resulting from genomics and structural proteomics, benchmarking and application of those tools. Furthermore, this platform integrated the genomic scale sequence and structural analysis and prediction tools with the advanced structure prediction and bioinformatics environment of ICM. The development of GAP was primarily oriented towards the annotation of new biomolecular structures using both structural and sequence data. Even though the amount of protein X-ray crystal data is growing exponentially, the volume of sequence data is growing even moremore » rapidly. This trend was exploited by leveraging the wealth of sequence data to provide functional annotation for protein structures. The additional information provided by GAP is expected to assist the majority of the commercial users of ICM, who are involved in drug discovery, in identifying promising drug targets as well in devising strategies for the rational design of therapeutics directed at the protein of interest. The GAP also provided valuable tools for biochemistry education, and structural genomics centers. In addition, GAP incorporates many novel prediction and analysis methods not available in other molecular modeling packages. This development led to signing the first Molsoft agreement in the structural genomics annotation area with the University of oxford Structural Genomics Center. This commercial agreement validated the Molsoft efforts under the GAP project and provided the basis for further development of the large scale functional annotation platform.« less

  7. Arizona Deafblind Project, 1995-1999. Final Report.

    ERIC Educational Resources Information Center

    Arizona State School for the Deaf and Blind, Tucson.

    This final report describes accomplishments of the four-year federally funded Arizona Deafblind Project which attempted to: (1) identify all deafblind children in Arizona; (2) deliver technical assistance to families; (3) deliver technical assistance to service providers; and (4) enhance community oversight, coordination, and collaboration with…

  8. DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle.

    PubMed

    Mikheikin, Andrey; Olsen, Anita; Leslie, Kevin; Russell-Pavier, Freddie; Yacoot, Andrew; Picco, Loren; Payton, Oliver; Toor, Amir; Chesney, Alden; Gimzewski, James K; Mishra, Bud; Reed, Jason

    2017-11-21

    Progress in whole-genome sequencing using short-read (e.g., <150 bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD optics to image DNA molecules with high-speed AFM. As a proof of principle, we used this new "nanomapping" method to detect and map precisely BCL2-IGH translocations present in lymph node biopsies of follicular lymphoma patents. This HS-AFM "nanomapping" technique can be complementary to both sequencing and other physical mapping approaches.

  9. Joint Common Architecture Demonstration (JCA Demo) Final Report

    DTIC Science & Technology

    2016-07-28

    approach for implementing open systems [16], formerly known as the Modular Open Systems Approach (MOSA). OSA is a business and technical strategy to... TECHNICAL REPORT RDMR-AD-16-01 JOINT COMMON ARCHITECTURE DEMONSTRATION (JCA DEMO) FINAL REPORT Scott A. Wigginton... Modular Avionics .......................................................................... 5 E. Model-Based Engineering

  10. Evaluation of Targeted Sequencing for Transcriptional Analysis of Archival Formalin-Fixed Paraffin-Embedded (FFPE) Samples

    EPA Science Inventory

    Next-generation sequencing provides unprecedented access to genomic information in archival FFPE tissue samples. However, costs and technical challenges related to RNA isolation and enrichment limit use of whole-genome RNA-sequencing for large-scale studies of FFPE specimens. Rec...

  11. 76 FR 80226 - Technical Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-23

    ... final rule, effective upon publication. Generally, the Administrative Procedure Act (APA) requires a.... Additionally, the APA requires that a final rule must have a delayed effective date of 30 days from the date of... delayed effective date requirement under the APA. 5 U.S.C. 553(d)(3). Again the technical change conforms...

  12. "Type Ia Supernovae: Tools for Studying Dark Energy" Final Technical Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Woosley, Stan; Kasen, Dan

    2017-05-10

    Final technical report for project "Type Ia Supernovae: Tools for the Study of Dark Energy" awarded jointly to scientists at the University of California, Santa Cruz and Berkeley, for computer modeling, theory and data analysis relevant to the use of Type Ia supernovae as standard candles for cosmology.

  13. 76 FR 50202 - National Technical Assistance and Dissemination Center for Children Who Are Deaf-Blind; Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-12

    ... DEPARTMENT OF EDUCATION [CFDA No. 84.326T] National Technical Assistance and Dissemination Center for Children Who Are Deaf-Blind; Final Extension of Project Period and Waiver AGENCY: Office of Special Education Programs, Office of Special Education and Rehabilitative Services, Department of...

  14. TADS Final Evaluation Report, 1980-81. Appendix S.

    ERIC Educational Resources Information Center

    Suarez, Tanya M.; And Others

    The document contains the final report of the Technical Assistance Development System (TADS), a program which provided technical assistance (TA) services to 53 Handicapped Children's Early Education Program (HCEEP) demonstration projects and 13 State Implementation Grants (SIGs). The evaluation report is divided into five sections. Section 1…

  15. Occupational Sequences: Auto Engines 1. AT 121.

    ERIC Educational Resources Information Center

    Korb, A. W.; And Others

    In an attempt to individualize an automotive course, the Vocational-Technical Division of Northern Montana College has developed Occupational Sequences for an engine rebuilding course. Occupational Sequences, a learning or teaching aid, is an analysis of numbered operations involved in engine rebuilding. Job sheets, included in the book, provide a…

  16. Fluorescence in situ hybridization and optical mapping to correct scaffold arrangement in the tomato genome

    USDA-ARS?s Scientific Manuscript database

    Modern biological analyses are often assisted by recent technologies making the sequencing of complex genomes both technically possible and feasible. We recently sequenced the tomato genome that, like many eukaryotic genomes, is large and complex. Current sequencing technologies allow the developmen...

  17. Rapid identification of causal mutations in tomato EMS populations via mapping-by-sequencing.

    PubMed

    Garcia, Virginie; Bres, Cécile; Just, Daniel; Fernandez, Lucie; Tai, Fabienne Wong Jun; Mauxion, Jean-Philippe; Le Paslier, Marie-Christine; Bérard, Aurélie; Brunel, Dominique; Aoki, Koh; Alseekh, Saleh; Fernie, Alisdair R; Fraser, Paul D; Rothan, Christophe

    2016-12-01

    The tomato is the model species of choice for fleshy fruit development and for the Solanaceae family. Ethyl methanesulfonate (EMS) mutants of tomato have already proven their utility for analysis of gene function in plants, leading to improved breeding stocks and superior tomato varieties. However, until recently, the identification of causal mutations that underlie particular phenotypes has been a very lengthy task that many laboratories could not afford because of spatial and technical limitations. Here, we describe a simple protocol for identifying causal mutations in tomato using a mapping-by-sequencing strategy. Plants displaying phenotypes of interest are first isolated by screening an EMS mutant collection generated in the miniature cultivar Micro-Tom. A recombinant F 2 population is then produced by crossing the mutant with a wild-type (WT; non-mutagenized) genotype, and F 2 segregants displaying the same phenotype are subsequently pooled. Finally, whole-genome sequencing and analysis of allele distributions in the pools allow for the identification of the causal mutation. The whole process, from the isolation of the tomato mutant to the identification of the causal mutation, takes 6-12 months. This strategy overcomes many previous limitations, is simple to use and can be applied in most laboratories with limited facilities for plant culture and genotyping.

  18. 04-ERD-052-Final Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Loots, G G; Ovcharenko, I; Collette, N

    2007-02-26

    Generating the sequence of the human genome represents a colossal achievement for science and mankind. The technical use for the human genome project information holds great promise to cure disease, prevent bioterror threats, as well as to learn about human origins. Yet converting the sequence data into biological meaningful information has not been immediately obvious, and we are still in the preliminary stages of understanding how the genome is organized, what are the functional building blocks and how do these sequences mediate complex biological processes. The overarching goal of this program was to develop novel methods and high throughput strategiesmore » for determining the functions of ''anonymous'' human genes that are evolutionarily deeply conserved in other vertebrates. We coupled analytical tool development and computational predictions regarding gene function with novel high throughput experimental strategies and tested biological predictions in the laboratory. The tools required for comparative genomic data-mining are fundamentally the same whether they are applied to scientific studies of related microbes or the search for functions of novel human genes. For this reason the tools, conceptual framework and the coupled informatics-experimental biology paradigm we developed in this LDRD has many potential scientific applications relevant to LLNL multidisciplinary research in bio-defense, bioengineering, bionanosciences and microbial and environmental genomics.« less

  19. 78 FR 57264 - Final Waiver and Extension of the Project Period for the Technical Assistance Coordination Center

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-18

    ...: 84.326Z.] Final Waiver and Extension of the Project Period for the Technical Assistance Coordination... project period. SUMMARY: The Secretary waives the requirements in the Education Department General Administrative Regulations that generally prohibit project periods exceeding five years and extensions of project...

  20. 77 FR 16923 - Agreements and Memoranda of Understanding Between the Food and Drug Administration and Other...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-23

    ...: Direct final rule. SUMMARY: This direct final rule makes technical changes that will update a requirement that many of our written agreements and memoranda of understanding (MOUs) with other departments..., accordingly, eliminates it. We are making these technical changes to conserve Agency time and resources...

  1. 1988 Underground Storage Tanks; Technical Requirements; Final Rule and Underground Storage Tanks Containing Petroleum-Financial Responsibility Requirements and State Program Approval Objective; Final Rule

    EPA Pesticide Factsheets

    EPA's 1988 regulations concerning USTs are contained in 40 CFR Part 280, 40 CFR Part 281 and 40 CFR Parts 282.50-282.105 and divided into three sections: technical requirements, financial responsibility requirements, and state program approval objectives.

  2. PITTSBURGH TECHNICAL HEALTH TRAINING INSTITUTE DEMONSTRATION PROJECT. FINAL REPORT, VOLUME II.

    ERIC Educational Resources Information Center

    KISHKUNAS, LOUIS J.

    APPENDIXES TO THE "FINAL REPORT," VOLUME I (VT 005 511), ARE INCLUDED--(1) A SCHEMATIC REPRESENTATION OF CURRICULUM DEVELOPMENT, (2) TECHNICAL BEHAVIOR CHECKLISTS, (3) PERFORMANCE INVENTORY FORMS USED IN ON-THE-JOB OBSERVATIONS, (4) REPORT FORM FOR TYPICAL JOB BEHAVIOR OF EMPLOYEE, (5) COOPERATING AREA HEALTH INSTITUTIONS, (6) TABLES OF Z SCORES…

  3. Technical-Oriented Enterprise Resource Planning (ERP) Body of Knowledge for Information Systems Programs: Content and Implementation

    ERIC Educational Resources Information Center

    Boyle, Todd A.

    2007-01-01

    In this article, the author proposes a body of knowledge that the educators can use to incorporate the technical aspects of enterprise resource planning (ERP) into an information systems (IS) program, encapsulated as the ERP technical knowledge framework. To illustrate the application of this framework, the author discusses a course sequence that…

  4. Invention and Writing in Technical Work: Representing the Object.

    ERIC Educational Resources Information Center

    Winsor, Dorothy A.

    1994-01-01

    Describes the way invention is relevant to the practice of technical writing. Studies three engineering students engaged in a real-world project. Shows how the students' technical work and invention for the final report were simultaneous activities. Claims that invention for and through writing overlaps with technical invention. (HB)

  5. Project T.E.A.M. (Technical Education Advancement Modules). Final Report.

    ERIC Educational Resources Information Center

    Greenville Technical Coll., SC.

    Project TEAM (Technical Education Advancement Modules), a cooperative demonstration program for high technology training, created an introductory technical training program and a consumer education package emphasizing the benefits of technical training. The curriculum and training focus of the project began with an assessment of employee needs in…

  6. Technical and economic evaluation of controlled disposal options for very low level radioactive wastes

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Robinson, P.J.; Vance, J.N.

    1990-08-01

    Over the past several years, there has been considerable interest by the nuclear industry in the Nuclear Regulatory Commission (NRC) explicitly defined an activity level in plant waste materials at which the radiological impacts would be so low as to be considered Below Regulatory Concern (BRC). In January 1989, Electric Power Research Institute (EPRI) completed an extensive industry research effort to develop the technical bases for establishing criteria for the disposal of very low activity wastes in ordinary disposal facilities. The Nuclear Management and Resources Council (NUMARC), with assistance from the Edison Electric Institute (EEI) and the Electric Power Researchmore » Institute (EPRI), drafted a petition titled: Petition for Rulemaking Regarding Disposal of Below Regulatory Concern Radioactive Wastes from Commercial Nuclear Power Plants.'' Subsequent to the industry making a final decision for submittal of the drafted BRC petition, EPRI was requested to evaluate the technical and economic impact of six BRC options. These options are: take no action in pursuing a BRC waste exemption, petition the NRC for authorization to disposal of any BRC waste in any ordinary disposal facility, limit disposal of BRC waste to the nuclear power plant site, limit disposal of BRC waste to the nuclear power plant site and other utility owned property, petition for a mixed waste exemption, and petition for single waste stream exemptions in sequence (i.e. soil, followed by sewage sludge, etc.). The petition and technical bases were written to support the disposal of any BRC waste type in any ordinary disposal facility. These documents do not provide all of the technical and economic information needed to completely assessment the BRC options. This report provides the technical and economic basis for a range of options concerning disposal of very low activity wastes. 3 figs., 20 tabs.« less

  7. 77 FR 39623 - Airworthiness Standards: Aircraft Engines; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ...] Airworthiness Standards: Aircraft Engines; Technical Amendment AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule; technical amendment. SUMMARY: This amendment clarifies aircraft engine... from applicants requesting FAA engine type certifications and aftermarket certifications, such as...

  8. 75 FR 13425 - Federal Acquisition Regulation; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... DEPARTMENT OF DEFENSE GENERAL SERVICES ADMINISTRATION NATIONAL AERONAUTICS AND SPACE ADMINISTRATION 48 CFR Part 14 [FAC 2005-39; Item VII; Docket FAR 2010-0078; Sequence 1] Federal Acquisition... publication schedules. Please cite FAC 2005-39, Technical Amendment. SUPPLEMENTARY INFORMATION: This document...

  9. Pooled-BAC sequencing of a black pod resistance region (cBPQTL12) in T. cacao

    USDA-ARS?s Scientific Manuscript database

    Whole genome sequencing (WGS) is an expensive and technically challenging endeavor. An alternative to WGS is to sequence specific chromosomal segments of biological interest (e.g. a QTL interval). This method is cheaper than WGS and reduces the risk of misassembly from distal parts of the genome. Us...

  10. The Limits of Informed Consent for an Overwhelmed Patient: Clinicians' Role in Protecting Patients and Preventing Overwhelm.

    PubMed

    Bester, Johan; Cole, Cristie M; Kodish, Eric

    2016-09-01

    In this paper, we examine the limits of informed consent with particular focus on ways in which various factors can overwhelm decision-making capacity. We introduce overwhelm as a phenomenon commonly experienced by patients in clinical settings and distinguish between emotional overwhelm and informational overload. We argue that in these situations, a clinician's primary duty is prevention of harm and suggest ways in which clinicians can discharge this obligation. To illustrate our argument, we consider the clinical application of genetic sequencing testing, which involves scientific and technical information that can compromise the understanding and decisional capacity of most patients. Finally, we consider and rebut objections that this could lead to paternalism. © 2016 American Medical Association. All Rights Reserved.

  11. 7 CFR 652.36 - Appeal of decertification decisions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... technical service provider's written appeal, the Chief or his designee, will make a final determination, in... CONSERVATION SERVICE, DEPARTMENT OF AGRICULTURE SUPPORT ACTIVITIES TECHNICAL SERVICE PROVIDER ASSISTANCE... of the State Conservationist's decertification determination, the technical service provider may...

  12. Taxonomic annotation of public fungal ITS sequences from the built environment – a report from an April 10–11, 2017 workshop (Aberdeen, UK)

    PubMed Central

    Nilsson, R. Henrik; Taylor, Andy F. S.; Adams, Rachel I.; Baschien, Christiane; Johan Bengtsson-Palme; Cangren, Patrik; Coleine, Claudia; Heide-Marie Daniel; Glassman, Sydney I.; Hirooka, Yuuri; Irinyi, Laszlo; Reda Iršėnaitė; Pedro M. Martin-Sanchez; Meyer, Wieland; Seung-Yoon Oh; Jose Paulo Sampaio; Seifert, Keith A.; Sklenář, Frantisek; Dirk Stubbe; Suh, Sung-Oui; Summerbell, Richard; Svantesson, Sten; Martin Unterseher; Cobus M. Visagie; Weiss, Michael; Woudenberg, Joyce HC; Christian Wurzbacher; den Wyngaert, Silke Van; Yilmaz, Neriman; Andrey Yurkov; Kõljalg, Urmas; Abarenkov, Kessy

    2018-01-01

    Abstract Recent DNA-based studies have shown that the built environment is surprisingly rich in fungi. These indoor fungi – whether transient visitors or more persistent residents – may hold clues to the rising levels of human allergies and other medical and building-related health problems observed globally. The taxonomic identity of these fungi is crucial in such pursuits. Molecular identification of the built mycobiome is no trivial undertaking, however, given the large number of unidentified, misidentified, and technically compromised fungal sequences in public sequence databases. In addition, the sequence metadata required to make informed taxonomic decisions – such as country and host/substrate of collection – are often lacking even from reference and ex-type sequences. Here we report on a taxonomic annotation workshop (April 10–11, 2017) organized at the James Hutton Institute/University of Aberdeen (UK) to facilitate reproducible studies of the built mycobiome. The 32 participants went through public fungal ITS barcode sequences related to the built mycobiome for taxonomic and nomenclatural correctness, technical quality, and metadata availability. A total of 19,508 changes – including 4,783 name changes, 14,121 metadata annotations, and the removal of 99 technically compromised sequences – were implemented in the UNITE database for molecular identification of fungi (https://unite.ut.ee/) and shared with a range of other databases and downstream resources. Among the genera that saw the largest number of changes were Penicillium, Talaromyces, Cladosporium, Acremonium, and Alternaria, all of them of significant importance in both culture-based and culture-independent surveys of the built environment. PMID:29559822

  13. Capturing Cognitive Fingerprints for Active Authentication

    DTIC Science & Technology

    2014-10-01

    CAPTURING COGNITIVE FINGERPRINTS FOR ACTIVE AUTHENTICATION IOWA STATE UNIVERSITY OF SCIENCE & TECHNOLOGY OCTOBER 2014 FINAL TECHNICAL REPORT...REPORT TYPE FINAL TECHNICAL REPORT 3. DATES COVERED (From - To) SEP 2013 – APR 2014 4. TITLE AND SUBTITLE CAPTURING COGNITIVE FINGERPRINTS FOR ACTIVE...The project ended before the IRB application was approved. 15. SUBJECT TERMS Active Authentication, Cognitive Fingerprints , Biometric Modalities

  14. Application of Communications Satellites to Educational Development. Final Technical Report, September 1, 1969-August 31, 1975.

    ERIC Educational Resources Information Center

    Morgan, Robert P.

    Research is summarized in a brief final report built around a four-section bibliography. The first section lists periodic progress reports and articles which provide an overview of the program, including articles which pertain primarily to educational rather than technical aspects of satellite utilization. Theses carried out in the fields of…

  15. Head Start Impact Study. Technical Report

    ERIC Educational Resources Information Center

    Puma, Michael; Bell, Stephen; Cook, Ronna; Heid, Camilla; Shapiro, Gary; Broene, Pam; Jenkins, Frank; Fletcher, Philip; Quinn, Liz; Friedman, Janet; Ciarico, Janet; Rohacek, Monica; Adams, Gina; Spier, Elizabeth

    2010-01-01

    This Technical Report is designed to provide technical detail to support the analysis and findings presented in the "Head Start Impact Study Final Report" (U.S. Department of Health and Human Services, January 2010). Chapter 1 provides an overview of the Head Start Impact Study and its findings. Chapter 2 provides technical information on the…

  16. Matching Community and Technical College Professional/Technical Education Capacity to Employer Demand. Final Report.

    ERIC Educational Resources Information Center

    Sommers, Paul; Heg, Deena

    A project was conducted to improve the state of Washington's community and technical college system by developing and using an improved occupational forecasting system to assess and respond to education and training needs. First, long-term occupational forecast data from Washington's Employment Security Department were matched with technical and…

  17. 75 FR 5784 - Guidance on Preparation of Market-Based Rate Filings and Electric Quarterly Reports by Public...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-04

    ... Preparation of Market-Based Rate Filings and Electric Quarterly Reports by Public Utilities; Notice of Technical Conference January 28, 2010. Take notice that Commission staff will convene a technical conference... final agenda of the technical conference. The March 3, 2010 technical conference will focus on the...

  18. A Revision of Technical Mathematics Based on the NCTM Standards. Final Report.

    ERIC Educational Resources Information Center

    Near, Barbara

    Between 1993 and 1996, Henry Ford Community College (Michigan) worked with business, industry, and technical instructors to revise their Technical Mathematics program in accordance with the National Council of Teachers of Mathematics (NCTM) Standards. The purpose of the project was to restructure the technical math curriculum and create a context…

  19. 76 FR 11327 - Technical Amendments to Rule 17a-8: Financial Recordkeeping and Reporting of Currency and Foreign...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-02

    ... SECURITIES AND EXCHANGE COMMISSION 17 CFR Part 240 [Release No. 34-63949] Technical Amendments to...: Securities and Exchange Commission. ACTION: Final rule; technical amendments. SUMMARY: The Securities and Exchange Commission (``Commission'') is adopting technical amendments to Rule 17a-8 under the Securities...

  20. Final priority; technical assistance to improve state data capacity--National Technical Assistance Center to improve state capacity to accurately collect and report IDEA data. Final priority.

    PubMed

    2013-05-20

    The Assistant Secretary for Special Education and Rehabilitative Services announces a priority under the Technical Assistance to Improve State Data Capacity program. The Assistant Secretary may use this priority for competitions in fiscal year (FY) 2013 and later years. We take this action to focus attention on an identified national need to provide technical assistance (TA) to States to improve their capacity to meet the data collection and reporting requirements of the Individuals with Disabilities Education Act (IDEA). We intend this priority to establish a TA center to improve State capacity to accurately collect and report IDEA data (Data Center).

  1. 77 FR 12948 - Federal Acquisition Regulation; Technical Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-02

    ... DEPARTMENT OF DEFENSE GENERAL SERVICES ADMINISTRATION NATIONAL AERONAUTICS AND SPACE ADMINISTRATION 48 CFR Parts 19, 42, and 52 [FAC 2005-56; Item VIII; Docket 2012-0079; Sequence 1] Federal... to status or publication schedules. Please cite FAC 2005-56, Technical Amendments. SUPPLEMENTARY...

  2. Technical Evaluation Motor No. 10 (TEM-10)

    NASA Technical Reports Server (NTRS)

    1993-01-01

    Technical Evaluation Motor No. 10 (TEM-10) was static fired on 27 Apr. 1993 at the Thiokol Corporation full-scale motor static test bay, T-24. This final test report documents the procedures, performance, and results of the static test firing of TEM-10. All observations, discussions, conclusions, and recommendations contained are final. Included is a presentation and discussion of TEM-10 performance, anomalies, and test results in concurrence with the objectives outlined in CTP-0110, Revision D, Space Shuttle Technical Evaluation Motor No. 10 (TEM-10) Static Fire Test Plan.

  3. Final priority; Technical Assistance on State Data Collection--IDEA Data Management Center. Final priority.

    PubMed

    2014-08-05

    The Assistant Secretary for the Office of Special Education and Rehabilitative Services (OSERS) announces a priority under the Technical Assistance on State Data Collection program. The Assistant Secretary may use this priority for competitions in fiscal year (FY) 2014 and later years. We take this action to fund a cooperative agreement to establish and operate an IDEA Data Management Center (Center) that will provide technical assistance (TA) to improve the capacity of States to meet the data collection requirements of the Individuals with Disabilities Education Act (IDEA).

  4. Technical Topic 3.2.2.d Bayesian and Non-Parametric Statistics: Integration of Neural Networks with Bayesian Networks for Data Fusion and Predictive Modeling

    DTIC Science & Technology

    2016-05-31

    and included explosives such as TATP, HMTD, RDX, RDX, ammonium nitrate , potassium perchlorate, potassium nitrate , sugar, and TNT. The approach...Distribution Unlimited UU UU UU UU 31-05-2016 15-Apr-2014 14-Jan-2015 Final Report: Technical Topic 3.2.2. d Bayesian and Non- parametric Statistics...of Papers published in non peer-reviewed journals: Final Report: Technical Topic 3.2.2. d Bayesian and Non-parametric Statistics: Integration of Neural

  5. Comparison of Three Instructional Sequences for the Addition and Subtraction Algorithms. Technical Report 273.

    ERIC Educational Resources Information Center

    Wiles, Clyde A.

    The study's purpose was to investigate the differential effects on the achievement of second-grade students that could be attributed to three instructional sequences for the learning of the addition and subtraction algorithms. One sequence presented the addition algorithm first (AS), the second presented the subtraction algorithm first (SA), and…

  6. Final June Revisions Rule Significant Contribution Assessment TSD

    EPA Pesticide Factsheets

    This Technical Support Document (TSD) presents quantitative assessments of the relationship between the final February revisions to the Transport Rule, the final June revisions rule, and the original analysis conducted for the final Transport Rule.

  7. Develop a Comprehensive Technical Training and Data Collection Program for Structural Welders and Fitters: Technical Proposal

    DTIC Science & Technology

    2006-05-30

    implementation Final Report 4 TECHNICAL PLAN AND RESULTS Task 1: Initiate the Project Management System Two senior NGSS production management...1 Technical Plan and Results...Third the system is hosted on a handheld unit which provides the foremen with an efficient daily planning tool. The Pilot System which entails

  8. Laser Technician Associate Degree Program. A Proposal Submitted to Wisconsin State Board of Vocational, Technical, and Adult Education. (Curriculum Development.) Final Report.

    ERIC Educational Resources Information Center

    North Central Technical Inst., Wausau, WI.

    This final report contains the program proposal with supporting data for developing curriculum materials for and implementing an associate-degree laser technology program at the North Central Technical Institute. The proposal outline provides this information: (1) objectives for the program designed to prepare a technician to safely operate,…

  9. California Deaf-Blind Services Final Report, October 1, 1995-September 30, 1999 [and] October 1, 1999-June 30, 2000 (No Cost Extension).

    ERIC Educational Resources Information Center

    Goertz, Lori; Franklin, Barbara

    This final report describes the activities and outcomes of the California Deaf-Blind Services (CDBS) program, a regionally based, family focused technical assistance and training project designed to improve services to children with deaf-blindness. The project conducted the following activities: (1) provided technical assistance to families and…

  10. New Jersey Technical Assistance Project (N.J. TAP): "Educational Resources and Support Services on Behalf of Children with Multiple Sensory Impairment." Final Report.

    ERIC Educational Resources Information Center

    New Jersey State Dept. of Education, Trenton.

    This final report describes activities and accomplishments of the New Jersey Technical Assistance Project, a project to improve educational resources and support services for students with multiple sensory impairment (deaf-blindness). Activities and accomplishments are presented in a tabular format for each project goal and objective. The project…

  11. 78 FR 77563 - Technical Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-24

    ... NATIONAL CREDIT UNION ADMINISTRATION 12 CFR Parts 700, 701, and 704 RIN 3133-AE33 Technical Amendments AGENCY: National Credit Union Administration (NCUA). ACTION: Final rule. SUMMARY: The NCUA Board... credit unions. The technical amendments conform the regulations to a recent policy change adopted by the...

  12. 75 FR 33682 - Export Administration Regulations; Technical Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-15

    ...-01] RIN 0694-AE93 Export Administration Regulations; Technical Amendments AGENCY: Bureau of Industry... Bureau of Industry and Security (BIS) makes a technical amendment to the Export Administration... review of final decisions and orders issued in BIS export control administrative enforcement proceedings...

  13. 78 FR 41331 - Defense Federal Acquisition Regulation Supplement; Technical Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-10

    ... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 225 Defense Federal Acquisition Regulation Supplement; Technical Amendments AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Final rule. SUMMARY: DoD is making technical amendment to the Defense...

  14. Final Revisions Rule Significant Contribution Assessment TSD

    EPA Pesticide Factsheets

    This Technical Support Document (TSD) presents quantitative assessments of the relationship between final revisions to the Transport Rule and the original analysis conducted for the final Transport Rule.

  15. 75 FR 15342 - Advisory Committees; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration 21 CFR Part 14 [Docket No. FDA-2010-N-0001] Advisory Committees; Technical Amendment Agency: Food and Drug Administration, HHS. ACTION: Final rule; technical amendment. SUMMARY: The Food and Drug Administration (FDA) is amending its...

  16. 2012 Technical Corrections Fact Sheet

    EPA Pesticide Factsheets

    Final Rule: 2012 Technical Corrections, Clarifying and Other Amendments to theGreenhouse Gas Reporting Rule, and Confidentiality Determinations for Certain DataElements of the Fluorinated Gas Source Category

  17. Ancient dna from pleistocene fossils: Preservation, recovery, and utility of ancient genetic information for quaternary research

    NASA Astrophysics Data System (ADS)

    Yang, Hong

    Until recently, recovery and analysis of genetic information encoded in ancient DNA sequences from Pleistocene fossils were impossible. Recent advances in molecular biology offered technical tools to obtain ancient DNA sequences from well-preserved Quaternary fossils and opened the possibilities to directly study genetic changes in fossil species to address various biological and paleontological questions. Ancient DNA studies involving Pleistocene fossil material and ancient DNA degradation and preservation in Quaternary deposits are reviewed. The molecular technology applied to isolate, amplify, and sequence ancient DNA is also presented. Authentication of ancient DNA sequences and technical problems associated with modern and ancient DNA contamination are discussed. As illustrated in recent studies on ancient DNA from proboscideans, it is apparent that fossil DNA sequence data can shed light on many aspects of Quaternary research such as systematics and phylogeny. conservation biology, evolutionary theory, molecular taphonomy, and forensic sciences. Improvement of molecular techniques and a better understanding of DNA degradation during fossilization are likely to build on current strengths and to overcome existing problems, making fossil DNA data a unique source of information for Quaternary scientists.

  18. Technical Report: Algorithm and Implementation for Quasispecies Abundance Inference with Confidence Intervals from Metagenomic Sequence Data

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McLoughlin, Kevin

    2016-01-11

    This report describes the design and implementation of an algorithm for estimating relative microbial abundances, together with confidence limits, using data from metagenomic DNA sequencing. For the background behind this project and a detailed discussion of our modeling approach for metagenomic data, we refer the reader to our earlier technical report, dated March 4, 2014. Briefly, we described a fully Bayesian generative model for paired-end sequence read data, incorporating the effects of the relative abundances, the distribution of sequence fragment lengths, fragment position bias, sequencing errors and variations between the sampled genomes and the nearest reference genomes. A distinctive featuremore » of our modeling approach is the use of a Chinese restaurant process (CRP) to describe the selection of genomes to be sampled, and thus the relative abundances. The CRP component is desirable for fitting abundances to reads that may map ambiguously to multiple targets, because it naturally leads to sparse solutions that select the best representative from each set of nearly equivalent genomes.« less

  19. Evaluation of Routine Atmospheric Sounding Measurements using Unmanned Systems (ERASMUS): Final Technical Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    de Boer, Gijs; Lawrence, Dale; Palo, Scott

    2017-03-29

    This final technical report details activities undertaken as part of the referenced project. Included is information on the preparation of aircraft for deployment to Alaska, summaries of the three deployments covered under this project, and a brief description of the dataset and science directions pursued. Additionally, we provide information on lessons learned, publications, and presentations resulting from this work.

  20. Vocational Curriculum Modification. Teaching Technical Language to Learning Handicapped Students. Project HIRE: A Curriculum Management System for Instructing the Handicapped. Final Report, Volume 1.

    ERIC Educational Resources Information Center

    Gardner, David C.; And Others

    Volume 1 of the final report on Project HIRE reports the design, development, field-testing, and refining of self-instructional packages to teach entry level technical vocabulary to learning handicapped students mainstreamed in vocational programs. Volume 2, a management handbook, reports the methods and findings concerning development of…

  1. Joint Technical Architecture for Robotic Systems (JTARS)-Final Report

    NASA Technical Reports Server (NTRS)

    Bradley, Arthur T.; Holloway, Sidney E., III

    2006-01-01

    This document represents the final report for the Joint Technical Architecture for Robotic Systems (JTARS) project, funded by the Office of Exploration as part of the Intramural Call for Proposals of 2005. The project was prematurely terminated, without review, as part of an agency-wide realignment towards the development of a Crew Exploration Vehicle (CEV) and meeting the near-term goals of lunar exploration.

  2. Review of Final Year MSP Evaluations, Performance Period 2007. Analytic and Technical Support for Mathematics and Science Partnerships

    ERIC Educational Resources Information Center

    Bobronnikov, Ellen; Rhodes, Hilary; Bradley, Cay

    2010-01-01

    This final report culminates the evaluation and technical assistance provided for the U.S. Department of Education's Mathematics and Science Partnership (MSP) Program and its projects since 2005. As part of this support, Abt Associates looked across the portfolio of projects funded by the MSP program to draw lessons on best practices. This…

  3. 76 FR 31416 - Federal Acquisition Regulation; Technical Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-31

    ... ADMINISTRATION 48 CFR Parts 52 and 53 [FAC 2005-52; Item VI; Docket 2011-0078; Sequence 2] Federal Acquisition... publication schedules. Please cite FAC 2005-52, Technical Amendments. SUPPLEMENTARY INFORMATION: In order to update certain elements in 48 CFR parts 52 and 53, this document makes editorial changes to the Federal...

  4. 75 FR 47566 - Office of Vocational and Adult Education; Overview Information; Promoting Rigorous Career and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-06

    ... (POSs) that link secondary and postsecondary education, combine academic and career and technical... careers, or both, and that include aligned academic and technical content; (f) Course Sequences: Course...: Systems that provide career counseling and academic advisory services to help students make informed...

  5. Pacific Educational Computer Network Study. Final Report.

    ERIC Educational Resources Information Center

    Hawaii Univ., Honolulu. ALOHA System.

    The Pacific Educational Computer Network Feasibility Study examined technical and non-technical aspects of the formation of an international Pacific Area computer network for higher education. The technical study covered the assessment of the feasibility of a packet-switched satellite and radio ground distribution network for data transmission…

  6. Impact of sequencing depth and read length on single cell RNA sequencing data of T cells.

    PubMed

    Rizzetto, Simone; Eltahla, Auda A; Lin, Peijie; Bull, Rowena; Lloyd, Andrew R; Ho, Joshua W K; Venturi, Vanessa; Luciani, Fabio

    2017-10-06

    Single cell RNA sequencing (scRNA-seq) provides great potential in measuring the gene expression profiles of heterogeneous cell populations. In immunology, scRNA-seq allowed the characterisation of transcript sequence diversity of functionally relevant T cell subsets, and the identification of the full length T cell receptor (TCRαβ), which defines the specificity against cognate antigens. Several factors, e.g. RNA library capture, cell quality, and sequencing output affect the quality of scRNA-seq data. We studied the effects of read length and sequencing depth on the quality of gene expression profiles, cell type identification, and TCRαβ reconstruction, utilising 1,305 single cells from 8 publically available scRNA-seq datasets, and simulation-based analyses. Gene expression was characterised by an increased number of unique genes identified with short read lengths (<50 bp), but these featured higher technical variability compared to profiles from longer reads. Successful TCRαβ reconstruction was achieved for 6 datasets (81% - 100%) with at least 0.25 millions (PE) reads of length >50 bp, while it failed for datasets with <30 bp reads. Sufficient read length and sequencing depth can control technical noise to enable accurate identification of TCRαβ and gene expression profiles from scRNA-seq data of T cells.

  7. Applications of Aptamers as Sensors

    NASA Astrophysics Data System (ADS)

    Cho, Eun Jeong; Lee, Joo-Woon; Ellington, Andrew D.

    2009-07-01

    Aptamers are ligand-binding nucleic acids whose affinities and selectivities can rival those of antibodies. They have been adapted to analytical applications not only as alternatives to antibodies, but as unique reagents in their own right. In particular, aptamers can be readily site-specifically modified during chemical or enzymatic synthesis to incorporate particular reporters, linkers, or other moieties. Also, aptamer secondary structures can be engineered to undergo analyte-dependent conformational changes, which, in concert with the ability to specifically place chemical agents, opens up a wealth of possible signal transduction schemas, irrespective of whether the detection modality is optical, electrochemical, or mass based. Finally, because aptamers are nucleic acids, they are readily adapted to sequence- (and hence signal-) amplification methods. However, application of aptamers without a basic knowledge of their biochemistry or technical requirements can cause serious analytical difficulties.

  8. Library Resources for Bac End Sequencing. Final Technical Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pieter J. de Jong

    2000-10-01

    Studies directed towards the specific aims outlined for this research award are summarized. The RPCI II Human Bac Library has been expanded by the addition of 6.9-fold genomic coverage. This segment has been generated from a MBOI partial digest of the same anonymous donor DNA used for the rest of the library. A new cloning vector, pTARBAC1, has been constructed and used in the construction of RPCI-II segment 5. This new cloning vector provides a new strategy in identifying targeted genomic regions and will greatly facilitate a large-scale analysis for positional cloning. A new maleCS7BC/6J mouse BAC library has beenmore » constructed. RPCI-23 contain 576 plates (approx 210,000 clones) and represents approximately 11-fold coverage of the mouse genome.« less

  9. Potential for Monitoring Gut Microbiota for Diagnosing Infections and Graft-versus-Host Disease in Cancer and Stem Cell Transplant Patients.

    PubMed

    Koh, Andrew Y

    2017-11-01

    Gut microbiota, the collective community of microorganisms inhabiting the intestine, have been shown to provide many beneficial functions for the host. Recent advances in next-generation sequencing and advanced molecular biology approaches have allowed researchers to identify gut microbiota signatures associated with disease processes and, in some cases, establish causality and elucidate underlying mechanisms. This report reviews 3 commonly used methods for studying the gut microbiota and microbiome (the collective genomes of the gut microorganisms): 16S rRNA gene sequencing, bacterial group or species-specific quantitative polymerase chain reaction (qPCR), and metagenomic shotgun sequencing (MSS). The technical approaches and resources needed for each approach are outlined, and advantages and disadvantages for each approach are summarized. The findings regarding the role of the gut microbiota in the health of patients with cancer and stem cell transplant (SCT) patients (specifically in modulating the development of gut-derived bacterial infections and a posttransplant immune-mediated complication known as graft-vs-host-disease) are reviewed. Finally, there is discussion of the potential viability of these approaches in the actual clinical treatment of cancer and SCT patients. Advances in next-generation sequencing have revolutionized our understanding of the importance of the gut microbiome to human health. Both 16S rRNA gene sequencing and MSS are currently too labor-intensive or computationally burdensome to incorporate into real-time clinical monitoring of gut microbiomes. Yet, the lessons learned from these technologies could be adapted to currently used methods (e.g., qPCR) that could then be rigorously tested in the clinical care of these patients. © 2017 American Association for Clinical Chemistry.

  10. Detection of microRNAs in color space.

    PubMed

    Marco, Antonio; Griffiths-Jones, Sam

    2012-02-01

    Deep sequencing provides inexpensive opportunities to characterize the transcriptional diversity of known genomes. The AB SOLiD technology generates millions of short sequencing reads in color-space; that is, the raw data is a sequence of colors, where each color represents 2 nt and each nucleotide is represented by two consecutive colors. This strategy is purported to have several advantages, including increased ability to distinguish sequencing errors from polymorphisms. Several programs have been developed to map short reads to genomes in color space. However, a number of previously unexplored technical issues arise when using SOLiD technology to characterize microRNAs. Here we explore these technical difficulties. First, since the sequenced reads are longer than the biological sequences, every read is expected to contain linker fragments. The color-calling error rate increases toward the 3(') end of the read such that recognizing the linker sequence for removal becomes problematic. Second, mapping in color space may lead to the loss of the first nucleotide of each read. We propose a sequential trimming and mapping approach to map small RNAs. Using our strategy, we reanalyze three published insect small RNA deep sequencing datasets and characterize 22 new microRNAs. A bash shell script to perform the sequential trimming and mapping procedure, called SeqTrimMap, is available at: http://www.mirbase.org/tools/seqtrimmap/ antonio.marco@manchester.ac.uk Supplementary data are available at Bioinformatics online.

  11. Community College Technical Mathematics Project. Final Report.

    ERIC Educational Resources Information Center

    Self, Samuel L.

    The purpose of the research project was to develop an applied or technical mathematics curriculum which would meet the needs of vocational-technical students at the community college level. The research project was divided into three distinct phases: Identifying the mathematical concepts requisite for job-entry competencies in each of the…

  12. 7 CFR 614.10 - Appeals before the Farm Service Agency county committee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... section are completed, provide the FSA county committee with a written technical determination in the form... part 780, a participant may appeal a final technical determination or a program decision to the FSA... appeal requests review of the technical determination by the applicable State Conservationist prior to...

  13. 77 FR 47495 - Final Priority; Technical Assistance on State Data Collection, Analysis, and Reporting-National...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-08

    ... intervention and preschool service providers with data on their qualifications, certification, and preparation... Priority; Technical Assistance on State Data Collection, Analysis, and Reporting--National IDEA Technical Assistance Center on Early Childhood Longitudinal Data Systems; Rule #0;#0;Federal Register / Vol. 77, No...

  14. 77 FR 18716 - Transportation Security Administration Postal Zip Code Change; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-28

    ... organizational changes and it has no substantive effect on the public. DATES: Effective March 28, 2012. FOR... No. 1572-9] Transportation Security Administration Postal Zip Code Change; Technical Amendment AGENCY: Transportation Security Administration, DHS. ACTION: Final rule. SUMMARY: This rule is a technical change to...

  15. Enrichment of target sequences for next-generation sequencing applications in research and diagnostics.

    PubMed

    Altmüller, Janine; Budde, Birgit S; Nürnberg, Peter

    2014-02-01

    Abstract Targeted re-sequencing such as gene panel sequencing (GPS) has become very popular in medical genetics, both for research projects and in diagnostic settings. The technical principles of the different enrichment methods have been reviewed several times before; however, new enrichment products are constantly entering the market, and researchers are often puzzled about the requirement to take decisions about long-term commitments, both for the enrichment product and the sequencing technology. This review summarizes important considerations for the experimental design and provides helpful recommendations in choosing the best sequencing strategy for various research projects and diagnostic applications.

  16. Studying the Cost and Value of Library Services: Final Report. Technical Report APLAB/94-3/1,2,3,4.

    ERIC Educational Resources Information Center

    Kantor, Paul B.; And Others

    This is the final technical report (in three parts) of a 15-month long project to study the costs and value of library functions at five major research libraries. Twenty-one services or service aspects were studied, and numerous measures of the importance or benefit of the service to the users were made. These measures were studied together to lay…

  17. An Inquiry into Testing of Information Retrieval Systems. Comparative Systems Laboratory Final Technical Report, Part III: CSL Related Studies.

    ERIC Educational Resources Information Center

    Zull, Carolyn Gifford, Ed.; And Others

    This third volume of the Comparative Systems Laboratory (CSL) Final Technical Report is a collection of relatively independent studies performed on CSL materials. Covered in this document are studies on: (1) properties of files, including a study of the growth rate of a dictionary of index terms as influenced by number of documents in the file and…

  18. Programs of Study as a State Policy Mandate: A Longitudinal Study of the South Carolina Personal Pathways to Success Initiative. Unabridged Final Technical Report (Years 1-5)

    ERIC Educational Resources Information Center

    Hammond, Cathy; Withington, Cairen; Sharp, Julia L.; Mobley, Catherine; Drew, Sam F.; Stringfield, Samuel C.; Stipanovic, Natalie; Swiger, Caroline M.; Daugherty, Lindsay; Griffith, Cathy

    2014-01-01

    This final report presents findings from data collection and analysis conducted during a five-year study by the National Dropout Prevention Center (NDPC) at Clemson University, in conjunction with colleagues from the National Research Center for Career and Technical Education (NRCCTE) at the University of Louisville. This project was one of three…

  19. Ground-Based Radiometric Measurements of Slant Path Attenuation in the V/W Bands

    DTIC Science & Technology

    2016-04-01

    GROUND-BASED RADIOMETRIC MEASUREMENTS OF SLANT PATH ATTENUATION IN THE V/W BANDS APRIL 2016 FINAL TECHNICAL REPORT APPROVED FOR PUBLIC RELEASE...2. REPORT TYPE FINAL TECHNICAL REPORT 3. DATES COVERED (From - To) OCT 2012 – SEP 2015 4. TITLE AND SUBTITLE GROUND-BASED RADIOMETRIC MEASUREMENTS ...SUPPLEMENTARY NOTES 14. ABSTRACT Ground-based radiometric techniques were applied to measure the slant path attenuation cumulative distribution function to

  20. Final Technical Progress Report: Development of Low-Cost Suspension Heliostat; December 7, 2011 - December 6, 2012

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bender, W.

    2013-01-01

    Final technical progress report of SunShot Incubator Solaflect Energy. The project succeeded in demonstrating that the Solaflect Suspension Heliostat design is viable for large-scale CSP installations. Canting accuracy is acceptable and is continually improving as Solaflect improves its understanding of this design. Cost reduction initiatives were successful, and there are still many opportunities for further development and further cost reduction.

  1. 33 CFR 385.30 - Master Implementation Sequencing Plan.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Incorporating New Information Into the Plan § 385.30 Master Implementation Sequencing Plan. (a) Not later than... projects of the Plan, including pilot projects and operational elements, based on the best scientific, technical, funding, contracting, and other information available. The Corps of Engineers and the South...

  2. Advanced Transportation System Studies Technical Area 2 (TA-2) Heavy Lift Launch Vehicle Development Contract. Volume 2; Technical Results

    NASA Technical Reports Server (NTRS)

    1995-01-01

    The purpose of the Advanced Transportation System Studies (ATSS) Technical Area 2 (TA-2) Heavy Lift Launch Vehicle Development contract was to provide advanced launch vehicle concept definition and analysis to assist NASA in the identification of future launch vehicle requirements. Contracted analysis activities included vehicle sizing and performance analysis, subsystem concept definition, propulsion subsystem definition (foreign and domestic), ground operations and facilities analysis, and life cycle cost estimation. This document is Volume 2 of the final report for the contract. It provides documentation of selected technical results from various TA-2 analysis activities, including a detailed narrative description of the SSTO concept assessment results, a user's guide for the associated SSTO sizing tools, an SSTO turnaround assessment report, an executive summary of the ground operations assessments performed during the first year of the contract, a configuration-independent vehicle health management system requirements report, a copy of all major TA-2 contract presentations, a copy of the FLO launch vehicle final report, and references to Pratt & Whitney's TA-2 sponsored final reports regarding the identification of Russian main propulsion technologies.

  3. An Analysis of Mathematics Course Sequences for Low Achieving Students at a Comprehensive Technical High School

    ERIC Educational Resources Information Center

    Edge, D. Michael

    2011-01-01

    This non-experimental study attempted to determine how the different prescribed mathematic tracks offered at a comprehensive technical high school influenced the mathematics performance of low-achieving students on standardized assessments of mathematics achievement. The goal was to provide an analysis of any statistically significant differences…

  4. Computer Education Curriculum. Connecticut Vocational Technical School System. Version 4.

    ERIC Educational Resources Information Center

    Kittell, Linda; Walczak, Joseph

    This computer education curriculum is designed specifically for Connecticut's Regional Vocational Technical Schools' grade 9 computer education course. Each of the 24 lessons is expected to cover at least one class period of 50 minutes. Introductory materials include a listing of course goals and objectives, an outline of sequence and scope via…

  5. International Students in the Scientific and Technical Writing Class.

    ERIC Educational Resources Information Center

    Constantinides, Janet C.

    A course sequence for teaching the forms and formats of scientific and technical writing to English as a second language (ESL) learners is described. The first assignment, a letter of application, serves as a diagnostic indication of the student's ability. The second assignment, a narrative, is designed to define the importance of audience and…

  6. Technical Evaluation Motor No. 7 (TEM-7)

    NASA Technical Reports Server (NTRS)

    Hughes, Phil

    1991-01-01

    The Technical Evaluation Motor No. 7 (TEM-7) test was a full-scale, full duration static test firing of a high performance motor-configuration solid rocket motor with nozzle vectoring. The final test report documents the procedures, performance, and results of the static test firing of TEM-7. All observations, discussions, conclusions, and recommendations included in the report are complete and final except for the TEM-7 fixed housing unbond investigation. A presentation and discussion of TEM-7 performance, anomalies, and test result concurrence with the objectives outlined in CTP-0107, Rev A, Space Shuttle Technical Evaluation Motor No. 7 (TEM-7) Static Fire Test Plan are included.

  7. Building Stronger State Energy Partnerships with the U.S. Department of Energy

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Marks, Kate

    2011-09-30

    This final technical report details the results of total work efforts and progress made from October 2007 – September 2011 under the National Association of State Energy Officials (NASEO) cooperative agreement DE-FC26-07NT43264, Building Stronger State Energy Partnerships with the U.S. Department of Energy. Major topical project areas in this final report include work efforts in the following areas: Energy Assurance and Critical Infrastructure, State and Regional Technical Assistance, Regional Initiative, Regional Coordination and Technical Assistance, and International Activities in China. All required deliverables have been provided to the National Energy Technology Laboratory and DOE program officials.

  8. Final Technical Report of Project DE-FG02-96ER14647

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lundeen, Stephen R.

    This is the final technical report of work completed under DOE support over the period Sept. 1, 1996 until May 31, 2015. The title of the project was "Ion/Excited Atom Collision Studies with a Rydberg Target and a CO2 Laser" from 9/1/96 to 10/31/06, and "Properties of Actinide Ions from Measurements of Rydberg Ion Fine Structure" from 11/1/06 until 5/31/15. The primary technical results were a detailed experimental study of resonant charge transfer between Rydberg atoms and highly-charged ions, and unique measurements of many properties of multiply-charged Thorium ions.

  9. BASiCS: Bayesian Analysis of Single-Cell Sequencing Data

    PubMed Central

    Vallejos, Catalina A.; Marioni, John C.; Richardson, Sylvia

    2015-01-01

    Single-cell mRNA sequencing can uncover novel cell-to-cell heterogeneity in gene expression levels in seemingly homogeneous populations of cells. However, these experiments are prone to high levels of unexplained technical noise, creating new challenges for identifying genes that show genuine heterogeneous expression within the population of cells under study. BASiCS (Bayesian Analysis of Single-Cell Sequencing data) is an integrated Bayesian hierarchical model where: (i) cell-specific normalisation constants are estimated as part of the model parameters, (ii) technical variability is quantified based on spike-in genes that are artificially introduced to each analysed cell’s lysate and (iii) the total variability of the expression counts is decomposed into technical and biological components. BASiCS also provides an intuitive detection criterion for highly (or lowly) variable genes within the population of cells under study. This is formalised by means of tail posterior probabilities associated to high (or low) biological cell-to-cell variance contributions, quantities that can be easily interpreted by users. We demonstrate our method using gene expression measurements from mouse Embryonic Stem Cells. Cross-validation and meaningful enrichment of gene ontology categories within genes classified as highly (or lowly) variable supports the efficacy of our approach. PMID:26107944

  10. BASiCS: Bayesian Analysis of Single-Cell Sequencing Data.

    PubMed

    Vallejos, Catalina A; Marioni, John C; Richardson, Sylvia

    2015-06-01

    Single-cell mRNA sequencing can uncover novel cell-to-cell heterogeneity in gene expression levels in seemingly homogeneous populations of cells. However, these experiments are prone to high levels of unexplained technical noise, creating new challenges for identifying genes that show genuine heterogeneous expression within the population of cells under study. BASiCS (Bayesian Analysis of Single-Cell Sequencing data) is an integrated Bayesian hierarchical model where: (i) cell-specific normalisation constants are estimated as part of the model parameters, (ii) technical variability is quantified based on spike-in genes that are artificially introduced to each analysed cell's lysate and (iii) the total variability of the expression counts is decomposed into technical and biological components. BASiCS also provides an intuitive detection criterion for highly (or lowly) variable genes within the population of cells under study. This is formalised by means of tail posterior probabilities associated to high (or low) biological cell-to-cell variance contributions, quantities that can be easily interpreted by users. We demonstrate our method using gene expression measurements from mouse Embryonic Stem Cells. Cross-validation and meaningful enrichment of gene ontology categories within genes classified as highly (or lowly) variable supports the efficacy of our approach.

  11. Attomole-level Genomics with Single-molecule Direct DNA, cDNA and RNA Sequencing Technologies.

    PubMed

    Ozsolak, Fatih

    2016-01-01

    With the introduction of next-generation sequencing (NGS) technologies in 2005, the domination of microarrays in genomics quickly came to an end due to NGS's superior technical performance and cost advantages. By enabling genetic analysis capabilities that were not possible previously, NGS technologies have started to play an integral role in all areas of biomedical research. This chapter outlines the low-quantity DNA and cDNA sequencing capabilities and applications developed with the Helicos single molecule DNA sequencing technology.

  12. 78 FR 34392 - Guidance for Industry and Food and Drug Administration Staff: Technical Considerations for Pen...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-07

    ...] Guidance for Industry and Food and Drug Administration Staff: Technical Considerations for Pen, Jet, and... availability of a final guidance document entitled ``Technical Considerations for Pen, Jet, and Related... developing information to support a marketing application for a pen, jet, or related injector device intended...

  13. Florida Study of Career and Technical Education. Final Report

    ERIC Educational Resources Information Center

    Jacobson, Louis; Mokher, Christine

    2014-01-01

    A key goal of the "Carl D. Perkins Career and Technical Education Act of 2006" ("Perkins IV") is to ensure career and technical education (CTE) programs are widely available for preparing high school and college students for "high skill, high wage, or high demand occupations in current or emerging professions"…

  14. Technical Failure of MR Elastography Examinations of the Liver: Experience from a Large Single-Center Study.

    PubMed

    Wagner, Mathilde; Corcuera-Solano, Idoia; Lo, Grace; Esses, Steven; Liao, Joseph; Besa, Cecilia; Chen, Nelson; Abraham, Ginu; Fung, Maggie; Babb, James S; Ehman, Richard L; Taouli, Bachir

    2017-08-01

    Purpose To assess the determinants of technical failure of magnetic resonance (MR) elastography of the liver in a large single-center study. Materials and Methods This retrospective study was approved by the institutional review board. Seven hundred eighty-one MR elastography examinations performed in 691 consecutive patients (mean age, 58 years; male patients, 434 [62.8%]) in a single center between June 2013 and August 2014 were retrospectively evaluated. MR elastography was performed at 3.0 T (n = 443) or 1.5 T (n = 338) by using a gradient-recalled-echo pulse sequence. MR elastography and anatomic image analysis were performed by two observers. Additional observers measured liver T2* and fat fraction. Technical failure was defined as no pixel value with a confidence index higher than 95% and/or no apparent shear waves imaged. Logistic regression analysis was performed to assess potential predictive factors of technical failure of MR elastography. Results The technical failure rate of MR elastography at 1.5 T was 3.5% (12 of 338), while it was higher, 15.3% (68 of 443), at 3.0 T. On the basis of univariate analysis, body mass index, liver iron deposition, massive ascites, use of 3.0 T, presence of cirrhosis, and alcoholic liver disease were all significantly associated with failure of MR elastography (P < .004); but on the basis of multivariable analysis, only body mass index, liver iron deposition, massive ascites, and use of 3.0 T were significantly associated with failure of MR elastography (P < .004). Conclusion The technical failure rate of MR elastography with a gradient-recalled-echo pulse sequence was low at 1.5 T but substantially higher at 3.0 T. Massive ascites, iron deposition, and high body mass index were additional independent factors associated with failure of MR elastography of the liver with a two-dimensional gradient-recalled-echo pulse sequence. © RSNA, 2017.

  15. Getting Limits off the Ground via Sequences

    ERIC Educational Resources Information Center

    Gass, Frederick

    2006-01-01

    Most beginning calculus courses spend little or no time on a technical definition of the limit concept. In most of the remaining courses, the definition presented is the traditional epsilon-delta definition. An alternative approach that bases the definition on infinite sequences has occasionally appeared in commercial textbooks but has not yet…

  16. Exploration of reticulate evolution in Amaryllidaceae tribe Hippeastreae (Asparagales) using sequence capture and NGS of low-copy nuclear markers

    USDA-ARS?s Scientific Manuscript database

    Technical Abstract Amaryllidaceae tribe Hippeastreae constitute a horticulturally valuable group of approximately 180 species of American petaloid monocots, characterized by dysploidy and polyploidy. A recent hypothesis based on ITS and chloroplast sequence data states that Hippeastreae experienced ...

  17. Final Technical Report for Award DESC0011912, "Trimodal Tapping Mode Atomic Force Microscopy: Simultaneous 4D Mapping of Conservative and Dissipative Probe-Sample Interactions of Energy-Relevant Materials”

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Solares, Santiago D.

    The final project report covering the period 7/1/14-6/30/17 provides an overview of the technical accomplishments in the areas of (i) fundamental viscoelasticity, (ii) multifrequency atomic force microscopy, and (iii) characterization of energy-relevant materials with atomic force microscopy. A list of publications supported by the project is also provided.

  18. Application of Knowledge-Based Techniques to Tracking Function

    DTIC Science & Technology

    2006-09-01

    38394041 42434445 46474849 505152 53545556 57585960 616263 646566 676869 707172 737475 7677 7879 8081 8283 8485 8687 8889 9091 9293 9495 969798 99100...Knowledge-based applications to adaptive space-time processing. Volume I: Summary”, AFRL-SN-TR-2001-146 Vol. I (of Vol. VI ), Final Technical Report, July...2001-146 Vol. IV (of Vol. VI ), Final Technical Report, July 2001. [53] C. Morgan, L. Moyer, “Knowledge-based applications to adaptive space-time

  19. Forecasting the Future Food Service World of Work. Final Report. Volume III. Technical Papers on the Future of the Food Service Industry. Service Management Reports.

    ERIC Educational Resources Information Center

    Powers, Thomas F., Ed.; Swinton, John R., Ed.

    This third and final volume of a study on the future of the food service industry contains the technical papers on which the information in the previous two volumes was based. The papers were written by various members of the Pennsylvania State University departments of economics, food science, nutrition, social psychology, and engineering and by…

  20. Final Technical Report for contract number DE-FG02-05ER15670

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Glazebrook, Jane

    This is the final technical report for contract number DE-FG02-05ER15670. The project is now complete, and results of the project have been published. Two papers were published based on work done in the last three-year funding period. The DOIs of these papers are included below. The abstracts of the papers, providing summaries of the work, are included in the body of the report.

  1. Astrobee Periodic Technical Review (PTR) Delta 3

    NASA Technical Reports Server (NTRS)

    Provencher, Christopher; Smith, Marion F.; Smith, Ernest Everett; Bualat, Maria Gabriele; Barlow, Jonathan Spencer

    2017-01-01

    Astrobee is a free flying robot for the inside of the International Space Station (ISS). The Periodic Technical Review (PTR) delta 3 is the final design review of the system presented to stakeholders.

  2. Polar source analysis : technical memorandum

    DOT National Transportation Integrated Search

    2017-09-29

    The following technical memorandum describes the development, testing and analysis of various polar source data sets. The memorandum also includes recommendation for potential inclusion in future releases of AEDT. This memorandum is the final deliver...

  3. 2005 v4.2 Technical Support Document

    EPA Pesticide Factsheets

    Technical Support Document for the Final Transport Rule describes how updated 2005 NEI, version 2 emissions and were processed for air quality modeling in support of the Cross-state Air Pollution Rule (CSAPR).

  4. Direct Final Rule for Technical Amendments for Marine Spark-Ignition Engines and Vessels

    EPA Pesticide Factsheets

    Rule published September 16, 2010 to make technical amendments to the design standard for portable marine fuel tanks. This rule incorporates safe recommended practices, developed through industry consensus.

  5. BlackOPs: increasing confidence in variant detection through mappability filtering.

    PubMed

    Cabanski, Christopher R; Wilkerson, Matthew D; Soloway, Matthew; Parker, Joel S; Liu, Jinze; Prins, Jan F; Marron, J S; Perou, Charles M; Hayes, D Neil

    2013-10-01

    Identifying variants using high-throughput sequencing data is currently a challenge because true biological variants can be indistinguishable from technical artifacts. One source of technical artifact results from incorrectly aligning experimentally observed sequences to their true genomic origin ('mismapping') and inferring differences in mismapped sequences to be true variants. We developed BlackOPs, an open-source tool that simulates experimental RNA-seq and DNA whole exome sequences derived from the reference genome, aligns these sequences by custom parameters, detects variants and outputs a blacklist of positions and alleles caused by mismapping. Blacklists contain thousands of artifact variants that are indistinguishable from true variants and, for a given sample, are expected to be almost completely false positives. We show that these blacklist positions are specific to the alignment algorithm and read length used, and BlackOPs allows users to generate a blacklist specific to their experimental setup. We queried the dbSNP and COSMIC variant databases and found numerous variants indistinguishable from mapping errors. We demonstrate how filtering against blacklist positions reduces the number of potential false variants using an RNA-seq glioblastoma cell line data set. In summary, accounting for mapping-caused variants tuned to experimental setups reduces false positives and, therefore, improves genome characterization by high-throughput sequencing.

  6. Sensitivity to sequencing depth in single-cell cancer genomics.

    PubMed

    Alves, João M; Posada, David

    2018-04-16

    Querying cancer genomes at single-cell resolution is expected to provide a powerful framework to understand in detail the dynamics of cancer evolution. However, given the high costs currently associated with single-cell sequencing, together with the inevitable technical noise arising from single-cell genome amplification, cost-effective strategies that maximize the quality of single-cell data are critically needed. Taking advantage of previously published single-cell whole-genome and whole-exome cancer datasets, we studied the impact of sequencing depth and sampling effort towards single-cell variant detection. Five single-cell whole-genome and whole-exome cancer datasets were independently downscaled to 25, 10, 5, and 1× sequencing depth. For each depth level, ten technical replicates were generated, resulting in a total of 6280 single-cell BAM files. The sensitivity of variant detection, including structural and driver mutations, genotyping, clonal inference, and phylogenetic reconstruction to sequencing depth was evaluated using recent tools specifically designed for single-cell data. Altogether, our results suggest that for relatively large sample sizes (25 or more cells) sequencing single tumor cells at depths > 5× does not drastically improve somatic variant discovery, characterization of clonal genotypes, or estimation of single-cell phylogenies. We suggest that sequencing multiple individual tumor cells at a modest depth represents an effective alternative to explore the mutational landscape and clonal evolutionary patterns of cancer genomes.

  7. Standards for Technological Literacy and STEM Education Delivery through Career and Technical Education Programs

    ERIC Educational Resources Information Center

    Asunda, Paul A.

    2012-01-01

    At a minimum, employers rely on career and technical education (CTE) and workforce training systems to supply workers able to perform in their jobs. In CTE classes that seek to integrate science, technology, engineering, and mathematics (STEM) concepts, it falls to the instructors to design and sequence the learning experiences that will promote…

  8. Newborn Sequencing in Genomic Medicine and Public Health

    PubMed Central

    Agrawal, Pankaj B.; Bailey, Donald B.; Beggs, Alan H.; Brenner, Steven E.; Brower, Amy M.; Cakici, Julie A.; Ceyhan-Birsoy, Ozge; Chan, Kee; Chen, Flavia; Currier, Robert J.; Dukhovny, Dmitry; Green, Robert C.; Harris-Wai, Julie; Holm, Ingrid A.; Iglesias, Brenda; Joseph, Galen; Kingsmore, Stephen F.; Koenig, Barbara A.; Kwok, Pui-Yan; Lantos, John; Leeder, Steven J.; Lewis, Megan A.; McGuire, Amy L.; Milko, Laura V.; Mooney, Sean D.; Parad, Richard B.; Pereira, Stacey; Petrikin, Joshua; Powell, Bradford C.; Powell, Cynthia M.; Puck, Jennifer M.; Rehm, Heidi L.; Risch, Neil; Roche, Myra; Shieh, Joseph T.; Veeraraghavan, Narayanan; Watson, Michael S.; Willig, Laurel; Yu, Timothy W.; Urv, Tiina; Wise, Anastasia L.

    2017-01-01

    The rapid development of genomic sequencing technologies has decreased the cost of genetic analysis to the extent that it seems plausible that genome-scale sequencing could have widespread availability in pediatric care. Genomic sequencing provides a powerful diagnostic modality for patients who manifest symptoms of monogenic disease and an opportunity to detect health conditions before their development. However, many technical, clinical, ethical, and societal challenges should be addressed before such technology is widely deployed in pediatric practice. This article provides an overview of the Newborn Sequencing in Genomic Medicine and Public Health Consortium, which is investigating the application of genome-scale sequencing in newborns for both diagnosis and screening. PMID:28096516

  9. Design and Analysis of Single-Cell Sequencing Experiments.

    PubMed

    Grün, Dominic; van Oudenaarden, Alexander

    2015-11-05

    Recent advances in single-cell sequencing hold great potential for exploring biological systems with unprecedented resolution. Sequencing the genome of individual cells can reveal somatic mutations and allows the investigation of clonal dynamics. Single-cell transcriptome sequencing can elucidate the cell type composition of a sample. However, single-cell sequencing comes with major technical challenges and yields complex data output. In this Primer, we provide an overview of available methods and discuss experimental design and single-cell data analysis. We hope that these guidelines will enable a growing number of researchers to leverage the power of single-cell sequencing. Copyright © 2015 Elsevier Inc. All rights reserved.

  10. Seattle To Portland Inter-City ITS Corridor Study And Communications Plan, Final Report

    DOT National Transportation Integrated Search

    1996-03-01

    THIS DOCUMENT IS THE FINAL REPORT PRESENTING THE SEATTLE TO PORTLAND INTELLIGENT TRANSPORTATION SYSTEM (ITS) EARLY DEPLOYMENT PLAN. THE FINAL REPORT SYNTHESIZES INFORMATION FROM TECHNICAL MEMORANDUMS 1 THROUGH 5; INCLUDING EXISTING AND FUTURE CONDITI...

  11. Benchmark Analysis of Career and Technical Education in Lenawee County. Final Report.

    ERIC Educational Resources Information Center

    Hollenbeck, Kevin

    The career and technical education (CTE) provided in grades K-12 in the county's vocational-technical center and 12 local public school districts of Lenawee County, Michigan, was benchmarked with respect to its attention to career development. Data were collected from the following sources: structured interviews with a number of key respondents…

  12. International Project on Technical and Vocational Education (UNEVOC). International Advisory Committee (Third Session, Paris, France, October 2-4, 1995). Final Report = Projet International pour l'Enseignement Technique et Professionnel (UNEVOC). Comite Consultatif International (Troisieme Session, Paris, France, 2-4 octobre 1995). Rapport Final.

    ERIC Educational Resources Information Center

    United Nations Educational, Scientific, and Cultural Organization, Paris (France).

    The international advisory committee of the International Project on Technical and Vocational Education (UNEVOC) held its third session in Paris in October 1995. Advisory committee members and observers from the United Nations' specialized, intergovernmental, and nongovernmental organizations reviewed the major UNEVOC project activities undertaken…

  13. International Project on Technical and Vocational Education (UNEVOC). International Advisory Committee (Second Session, Paris, France, December 12-14, 1994). Final Report = Projet International pour l'Enseignement Technique et Professionnel (UNEVOC). Comite Consultatif International (Deuxieme Session, Paris, France, 12-14 decembre 1994). Rapport Final.

    ERIC Educational Resources Information Center

    United Nations Educational, Scientific, and Cultural Organization, Paris (France).

    The international advisory committee of the International Project on Technical and Vocational Education (UNEVOC) held its second session in Paris in December 1994. Nine advisory committee members and observers from the United Nations' specialized, intergovernmental, and nongovernmental organizations reviewed the major UNEVOC project activities…

  14. Final Technical Report for ARRA Funding

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rusack, Roger; Mans, Jeremiah; Poling, Ronald

    Final technical report of the University of Minnesota experimental high energy physics group for ARRA support. The Cryogenic Dark Matter Experiment (CDMS) used the funds received to construct a new passive shield to protect a high-purity germanium detector located in the Soudan mine in Northern Minnesota from cosmic rays. The BESIII and the CMS groups purchased computing hardware to assemble computer farms for data analysis and to generate large volumes of simulated data for comparison with the data collected.

  15. Making a Difference in the Lives of Infants and Toddlers and Their Families: The Impacts of Early Head Start. Volumes I-III: Final Technical Report [and] Appendixes [and] Local Contributions to Understanding the Programs and Their Impacts.

    ERIC Educational Resources Information Center

    Love, John M.; Kisker, Ellen Eliason; Ross, Christine M.; Schochet, Peter Z.; Brooks-Gunn, Jeanne; Paulsell, Diane; Boller, Kimberly; Constantine, Jill; Vogel, Cheri; Fuligni, Alison Sidle; Brady-Smith, Christy

    Early Head Start was designed in 1994 as a 2-generation program to enhance children's development and health, strengthen family and community partnerships, and support the staff delivering new services to low-income families with pregnant women, infants, or toddlers. This document contains the final technical report, appendixes, and local…

  16. International Project on Technical and Vocational Education (UNEVOC). International Advisory Committee (First Session, Berlin, Germany, September 20-22, 1993). Final Report = Projet International pour l'Enseignement Technique et Professionnel (UNEVOC). Comite Consultatif International (Premiere Session, Berlin, Germany, 20-22 septembre 1993). Rapport Final.

    ERIC Educational Resources Information Center

    United Nations Educational, Scientific, and Cultural Organization, Paris (France).

    The international advisory committee of the International Project on Technical and Vocational Education (UNEVOC) held its first session in Berlin in September 1993. The advisory committee's 10 members and observers from the United Nations' specialized, intergovernmental, and nongovernmental organizations discussed educational policy, links between…

  17. [Engineering a bone free flap for maxillofacial reconstruction: technical restrictions].

    PubMed

    Raoul, G; Myon, L; Chai, F; Blanchemain, N; Ferri, J

    2011-09-01

    Vascularisation is a key for success in bone tissue engineering. Creating a functional vascular network is an important concern so as to ensure vitality in regenerated tissues. Many strategies were developed to achieve this goal. One of these is cellular growth technique by perfusion bioreactor chamber. These new technical requirements came along with improved media and chamber receptacles: bioreactors (chapter 2). Some bone tissue engineering processes already have clinical applications but for volumes limited by the lack of vascularisation. Resorbable or non-resorbable membranes are an example. They are used separately or in association with bone grafts and they protect the graft during the revascularization process. Potentiated osseous regeneration uses molecular or cellular adjuvants (BMPs and autologous stem cells) to improve osseous healing. Significant improvements were made: integration of specific sequences, which may guide and enhance cells differentiation in scaffold; nano- or micro-patterned cell containing scaffolds. Finally, some authors consider the patient body as an ideal bioreactor to induce vascularisation in large volumes of grafted tissues. "Endocultivation", i.e., cellular culture inside the human body was proven to be feasible and safe. The properties of regenerated bone in the long run remain to be assessed. The objective to reach remains the engineering of an "in vitro" osseous free flap without morbidity. Copyright © 2011 Elsevier Masson SAS. All rights reserved.

  18. Key findings and remaining questions in the areas of core-concrete interaction and debris coolability

    DOE PAGES

    Farmer, M. T.; Gerardi, C.; Bremer, N.; ...

    2016-10-31

    The reactor accidents at Fukushima-Dai-ichi have rekindled interest in late phase severe accident behavior involving reactor pressure vessel breach and discharge of molten core melt into the containment. Two technical issues of interest in this area include core-concrete interaction and the extent to which the core debris may be quenched and rendered coolable by top flooding. The OECD-sponsored Melt Coolability and Concrete Interaction (MCCI) programs at Argonne National Laboratory included the conduct of large scale reactor material experiments and associated analysis with the objectives of resolving the ex-vessel debris coolability issue, and to address remaining uncertainties related to long-term two-dimensionalmore » molten core-concrete interactions under both wet and dry cavity conditions. These tests provided a broad database to support accident management planning, as well as the development and validation of models and codes that can be used to extrapolate the experiment results to plant conditions. This paper provides a high level overview of the key experiment results obtained during the program. Finally, a discussion is also provided that describes technical gaps that remain in this area, several of which have arisen based on the sequence of events and operator actions during Fukushima.« less

  19. Study of lubricant circulation in HVAC systems. Volume 1: Description of technical effort and results; Final technical report, March 1995--April 1996

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Biancardi, F.R.; Michels, H.H.; Sienel, T.H.

    1996-10-01

    The purpose of this program was to conduct experimental and analytical efforts to determine lubricant circulation characteristics of new HFC/POE pairs and HFC/mineral oil pairs in a representative central residential HVAC system and to compare their behavior with the traditional HCFC-22/mineral oil (refrigerant/lubricant) pair. A dynamic test facility was designed and built to conduct the experimental efforts. This facility provided a unique capability to visually and physically measure oil circulation rates, on-line, in operating systems. A unique on-line ultraviolet-based measurement device was used to obtain detailed data on the rate and level of lubricant oil circulated within the operating heatmore » pump system. The experimental and analytical data developed during the program are presented as a function of vapor velocity, refrigerant/lubricant viscosity, system features and equipment. Both visual observations and instrumentation were used to understand ``worst case`` oil circulation situations. This report is presented in two volumes. Volume 1 contains a complete description of the program scope, objective, test results summary, conclusions, description of test facility and recommendations for future effort. Volume 2 contains all of the program test data essentially as taken from the laboratory dynamic test facility during the sequence of runs.« less

  20. Key findings and remaining questions in the areas of core-concrete interaction and debris coolability

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Farmer, M. T.; Gerardi, C.; Bremer, N.

    The reactor accidents at Fukushima-Dai-ichi have rekindled interest in late phase severe accident behavior involving reactor pressure vessel breach and discharge of molten core melt into the containment. Two technical issues of interest in this area include core-concrete interaction and the extent to which the core debris may be quenched and rendered coolable by top flooding. The OECD-sponsored Melt Coolability and Concrete Interaction (MCCI) programs at Argonne National Laboratory included the conduct of large scale reactor material experiments and associated analysis with the objectives of resolving the ex-vessel debris coolability issue, and to address remaining uncertainties related to long-term two-dimensionalmore » molten core-concrete interactions under both wet and dry cavity conditions. These tests provided a broad database to support accident management planning, as well as the development and validation of models and codes that can be used to extrapolate the experiment results to plant conditions. This paper provides a high level overview of the key experiment results obtained during the program. Finally, a discussion is also provided that describes technical gaps that remain in this area, several of which have arisen based on the sequence of events and operator actions during Fukushima.« less

  1. Dynamic Tunneling Junctions at the Atomic Intersection of Two Twisted Graphene Edges.

    PubMed

    Bellunato, Amedeo; Vrbica, Sasha D; Sabater, Carlos; de Vos, Erik W; Fermin, Remko; Kanneworff, Kirsten N; Galli, Federica; van Ruitenbeek, Jan M; Schneider, Grégory F

    2018-04-11

    The investigation of the transport properties of single molecules by flowing tunneling currents across extremely narrow gaps is relevant for challenges as diverse as the development of molecular electronics and sequencing of DNA. The achievement of well-defined electrode architectures remains a technical challenge, especially due to the necessity of high precision fabrication processes and the chemical instability of most bulk metals. Here, we illustrate a continuously adjustable tunneling junction between the edges of two twisted graphene sheets. The unique property of the graphene electrodes is that the sheets are rigidly supported all the way to the atomic edge. By analyzing the tunneling current characteristics, we also demonstrate that the spacing across the gap junction can be controllably adjusted. Finally, we demonstrate the transition from the tunneling regime to contact and the formation of an atomic-sized junction between the two edges of graphene.

  2. Relevant principal factors affecting the reproducibility of insect primary culture.

    PubMed

    Ogata, Norichika; Iwabuchi, Kikuo

    2017-06-01

    The primary culture of insect cells often suffers from problems with poor reproducibility in the quality of the final cell preparations. The cellular composition of the explants (cell number and cell types), surgical methods (surgical duration and surgical isolation), and physiological and genetic differences between donors may be critical factors affecting the reproducibility of culture. However, little is known about where biological variation (interindividual differences between donors) ends and technical variation (variance in replication of culture conditions) begins. In this study, we cultured larval fat bodies from the Japanese rhinoceros beetle, Allomyrina dichotoma, and evaluated, using linear mixed models, the effect of interindividual variation between donors on the reproducibility of the culture. We also performed transcriptome analysis of the hemocyte-like cells mainly seen in the cultures using RNA sequencing and ultrastructural analyses of hemocytes using a transmission electron microscope, revealing that the cultured cells have many characteristics of insect hemocytes.

  3. Final Technical Report for Award # ER64999

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Metcalf, William W.

    2014-10-08

    This report provides a summary of activities for Award # ER64999, a Genomes to Life Project funded by the Office of Science, Basic Energy Research. The project was entitled "Methanogenic archaea and the global carbon cycle: a systems biology approach to the study of Methanosarcina species". The long-term goal of this multi-investigator project was the creation of integrated, multiscale models that accurately and quantitatively predict the role of Methanosarcina species in the global carbon cycle under dynamic environmental conditions. To achieve these goals we pursed four specific aims: (1) genome sequencing of numerous members of the Order Methanosarcinales, (2) identificationmore » of genomic sources of phenotypic variation through in silico comparative genomics, (3) elucidation of the transcriptional networks of two Methanosarcina species, and (4) development of comprehensive metabolic network models for characterized strains to address the question of how metabolic models scale with genetic distance.« less

  4. Massively parallel digital transcriptional profiling of single cells

    PubMed Central

    Zheng, Grace X. Y.; Terry, Jessica M.; Belgrader, Phillip; Ryvkin, Paul; Bent, Zachary W.; Wilson, Ryan; Ziraldo, Solongo B.; Wheeler, Tobias D.; McDermott, Geoff P.; Zhu, Junjie; Gregory, Mark T.; Shuga, Joe; Montesclaros, Luz; Underwood, Jason G.; Masquelier, Donald A.; Nishimura, Stefanie Y.; Schnall-Levin, Michael; Wyatt, Paul W.; Hindson, Christopher M.; Bharadwaj, Rajiv; Wong, Alexander; Ness, Kevin D.; Beppu, Lan W.; Deeg, H. Joachim; McFarland, Christopher; Loeb, Keith R.; Valente, William J.; Ericson, Nolan G.; Stevens, Emily A.; Radich, Jerald P.; Mikkelsen, Tarjei S.; Hindson, Benjamin J.; Bielas, Jason H.

    2017-01-01

    Characterizing the transcriptome of individual cells is fundamental to understanding complex biological systems. We describe a droplet-based system that enables 3′ mRNA counting of tens of thousands of single cells per sample. Cell encapsulation, of up to 8 samples at a time, takes place in ∼6 min, with ∼50% cell capture efficiency. To demonstrate the system's technical performance, we collected transcriptome data from ∼250k single cells across 29 samples. We validated the sensitivity of the system and its ability to detect rare populations using cell lines and synthetic RNAs. We profiled 68k peripheral blood mononuclear cells to demonstrate the system's ability to characterize large immune populations. Finally, we used sequence variation in the transcriptome data to determine host and donor chimerism at single-cell resolution from bone marrow mononuclear cells isolated from transplant patients. PMID:28091601

  5. Every factor helps: Rapid Ptychographic Reconstruction

    NASA Astrophysics Data System (ADS)

    Nashed, Youssef

    2015-03-01

    Recent advances in microscopy, specifically higher spatial resolution and data acquisition rates, require faster and more robust phase retrieval reconstruction methods. Ptychography is a phase retrieval technique for reconstructing the complex transmission function of a specimen from a sequence of diffraction patterns in visible light, X-ray, and electron microscopes. As technical advances allow larger fields to be imaged, computational challenges arise for reconstructing the correspondingly larger data volumes. Waiting to postprocess datasets offline results in missed opportunities. Here we present a parallel method for real-time ptychographic phase retrieval. It uses a hybrid parallel strategy to divide the computation between multiple graphics processing units (GPUs). A final specimen reconstruction is then achieved by different techniques to merge sub-dataset results into a single complex phase and amplitude image. Results are shown on a simulated specimen and real datasets from X-ray experiments conducted at a synchrotron light source.

  6. Phase magnification by two-axis countertwisting for detection-noise robust interferometry

    NASA Astrophysics Data System (ADS)

    Anders, Fabian; Pezzè, Luca; Smerzi, Augusto; Klempt, Carsten

    2018-04-01

    Entanglement-enhanced atom interferometry has the potential of surpassing the standard quantum limit and eventually reaching the ultimate Heisenberg bound. The experimental progress is, however, hindered by various technical noise sources, including the noise in the detection of the output quantum state. The influence of detection noise can be largely overcome by exploiting echo schemes, where the entanglement-generating interaction is repeated after the interferometer sequence. Here, we propose an echo protocol that uses two-axis countertwisting as the main nonlinear interaction. We demonstrate that the scheme is robust to detection noise and its performance is superior compared to the already demonstrated one-axis twisting echo scheme. In particular, the sensitivity maintains the Heisenberg scaling in the limit of a large particle number. Finally, we show that the protocol can be implemented with spinor Bose-Einstein condensates. Our results thus outline a realistic approach to mitigate the detection noise in quantum-enhanced interferometry.

  7. Dynamic Tunneling Junctions at the Atomic Intersection of Two Twisted Graphene Edges

    PubMed Central

    2018-01-01

    The investigation of the transport properties of single molecules by flowing tunneling currents across extremely narrow gaps is relevant for challenges as diverse as the development of molecular electronics and sequencing of DNA. The achievement of well-defined electrode architectures remains a technical challenge, especially due to the necessity of high precision fabrication processes and the chemical instability of most bulk metals. Here, we illustrate a continuously adjustable tunneling junction between the edges of two twisted graphene sheets. The unique property of the graphene electrodes is that the sheets are rigidly supported all the way to the atomic edge. By analyzing the tunneling current characteristics, we also demonstrate that the spacing across the gap junction can be controllably adjusted. Finally, we demonstrate the transition from the tunneling regime to contact and the formation of an atomic-sized junction between the two edges of graphene. PMID:29513997

  8. Illinois Clean Coal Institute 2005 annual report. Final technical report for the period September 1st, 2004, through August 31, 2005 on projects funded by the Illinois Department of Commerce and Economic Opportunity

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    2005-11-08

    This final technical report contains the abstracts and executive summaries of projects funded through the Illinois Clean Coal Institute solicitation entitled 'Request for proposals No. 04-1(ICCI/RFP04-1)'. Support of these projects is by the Office of Coal Development and Department of Commerce and Economic Opportunity. The projects fall into the following categories: advanced coal mining technologies; coal preparation and coal production business practice; management of coal combustion byproducts; commercialization and technology transfer. Final project extensions are also recorded.

  9. Repeated Kicking Actions in Karate: Effect on Technical Execution in Elite Practitioners.

    PubMed

    Quinzi, Federico; Camomilla, Valentina; Di Mario, Alberto; Felici, Francesco; Sbriccoli, Paola

    2016-04-01

    Training in martial arts is commonly performed by repeating a technical action continuously for a given number of times. This study aimed to investigate if the repetition of the task alters the proper technical execution, limiting the training efficacy for the technical evaluation during competition. This aim was pursued analyzing lower-limb kinematics and muscle activation during repeated roundhouse kicks. Six junior karate practitioners performed continuously 20 repetitions of the kick. Hip and knee kinematics and sEMG of vastus lateralis, biceps (BF), and rectus femoris were recorded. For each repetition, hip abduction-adduction and flexion-extension and knee flexion-extension peak angular displacements and velocities, agonist and antagonist muscle activation were computed. Moreover, to monitor for the presence of myoelectric fatigue, if any, the median frequency of the sEMG was computed. All variables were normalized with respect to their individual maximum observed during the sequence of kicks. Linear regressions were fitted to each normalized parameter to test its relationship with the repetition number. Linear-regression analysis showed that, during the sequence, the athletes modified their technique: Knee flexion, BF median frequency, hip abduction, knee-extension angular velocity, and BF antagonist activation significantly decreased. Conversely, hip flexion increased significantly. Since karate combat competitions require proper technical execution, training protocols combining severe fatigue and technical actions should be carefully proposed because of technique adaptations. Moreover, trainers and karate masters should consider including specific strength exercises for the BF and more generally for knee flexors.

  10. Bringing Next-Generation Sequencing into the Classroom through a Comparison of Molecular Biology Techniques

    ERIC Educational Resources Information Center

    Bowling, Bethany; Zimmer, Erin; Pyatt, Robert E.

    2014-01-01

    Although the development of next-generation (NextGen) sequencing technologies has revolutionized genomic research and medicine, the incorporation of these topics into the classroom is challenging, given an implied high degree of technical complexity. We developed an easy-to-implement, interactive classroom activity investigating the similarities…

  11. Applications of the Functional Writing Model in Technical and Professional Writing.

    ERIC Educational Resources Information Center

    Brostoff, Anita

    The functional writing model is a method by which students learn to devise and organize a written argument. Salient features of functional writing include the organizing idea (a component that logically unifies a paragraph or sequence of paragraphs), the reader's frame of reference, forecasting (prediction of the sequence by which the organizing…

  12. High-resolution ultrashort echo time (UTE) imaging on human knee with AWSOS sequence at 3.0 T.

    PubMed

    Qian, Yongxian; Williams, Ashley A; Chu, Constance R; Boada, Fernando E

    2012-01-01

    To demonstrate the technical feasibility of high-resolution (0.28-0.14 mm) ultrashort echo time (UTE) imaging on human knee at 3T with the acquisition-weighted stack of spirals (AWSOS) sequence. Nine human subjects were scanned on a 3T MRI scanner with an 8-channel knee coil using the AWSOS sequence and isocenter positioning plus manual shimming. High-resolution UTE images were obtained on the subject knees at TE = 0.6 msec with total acquisition time of 5.12 minutes for 60 slices at an in-plane resolution of 0.28 mm and 10.24 minutes for 40 slices at an in-plane resolution of 0.14 mm. Isocenter positioning, manual shimming, and the 8-channel array coil helped minimize image distortion and achieve high signal-to-noise ratio (SNR). It is technically feasible on a clinical 3T MRI scanner to perform UTE imaging on human knee at very high spatial resolutions (0.28-0.14 mm) within reasonable scan time (5-10 min) using the AWSOS sequence. Copyright © 2011 Wiley Periodicals, Inc.

  13. Renewable Fuel Standard (RFS2): Final Rule Additional Resources

    EPA Pesticide Factsheets

    The final rule of fuels and fuel additives: renewable fuel standard program is published on March 26, 2010 and is effective on July 1, 2010. You will find the links to this final rule and technical amendments supporting this rule.

  14. 75 FR 48273 - Technical Service Provider Assistance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-10

    ... DEPARTMENT OF AGRICULTURE Natural Resources Conservation Service 7 CFR Part 652 RIN 0578-AA48 Technical Service Provider Assistance AGENCY: Natural Resources Conservation Service, United States Department of Agriculture. ACTION: Final rule; Correcting amendment. SUMMARY: The Natural Resources...

  15. Partial Withdrawal and Final Rule for Nonroad Technical Amendments

    EPA Pesticide Factsheets

    Amendments to the technical hardship provisions under the Transition Program for Equipment Manufacturers related to the Tier 4 standards for nonroad diesel engines, and to the replacement engine exemption generally applicable to new nonroad engines.

  16. Sequencing technologies - the next generation.

    PubMed

    Metzker, Michael L

    2010-01-01

    Demand has never been greater for revolutionary technologies that deliver fast, inexpensive and accurate genome information. This challenge has catalysed the development of next-generation sequencing (NGS) technologies. The inexpensive production of large volumes of sequence data is the primary advantage over conventional methods. Here, I present a technical review of template preparation, sequencing and imaging, genome alignment and assembly approaches, and recent advances in current and near-term commercially available NGS instruments. I also outline the broad range of applications for NGS technologies, in addition to providing guidelines for platform selection to address biological questions of interest.

  17. A Pilot Program to Provide Ownership Skills to Seniors in the Vocational Technical Schools and Other Educational Institutions, Year Two. Final Report.

    ERIC Educational Resources Information Center

    Norwich Regional Vocational Technical School, CT.

    Responding to a Small Business Administration statement that technical school graduates lack knowledge of business principles, the Norwich (Connecticut) Regional Vocational Technical School conducted a course in small business ownership/management for all of its seniors. The required course, a fifty-four-hour module, replaced one English,…

  18. A Case Study of Technical Assistance to Demonstration Programs for Young Handicapped Children. Part III.

    ERIC Educational Resources Information Center

    Behr, Shirley K.; And Others

    The report describes the third and final year of a 3-year case study of the technical assistance process as implemented by the Technical Assistance Development System (TADS) for the staffs of two demonstration programs for preschool handicapped children and their families. Following a review of TADS and the two demonstration programs, the…

  19. Chemical Durability Improvement and Static Fatigue of Glasses.

    DTIC Science & Technology

    1982-08-01

    Afl-Alla 837 RENSSELAER POLYIECmfJ!C INST TRtOY NY DEPT OF MATERIAL--ETC F/6 ii/ CHEMICAL DURABILITY IMPROVEMENT AND STATIC FATIGUE OF GLASSESW AUC2...82 M TOMOZAWA NOGGIN 7A-C-0315 UNC LASS IF IED N ENEEEEEE FINAL TECHNICAL REPORT For the period April 1, 1978 "u March 31, 198200 CHEMICAL DURABILITY...REPORT A PERIOD COVERED Chemical Durability Improvement and Static Final Technical Report Fatiue o GlasesApril 1, 1978"’,March 31, 1982 S. PERFORMING ORG

  20. Experimental Program Final Technical Progress Report: 15 February 2007 to 30 September 2012

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kinney, Edward R.

    2014-09-12

    This is the final technical report of the grant DE-FG02-04ER41301 to the University of Colorado at Boulder entitled "Intermediate Energy Nuclear Physics" and describes the results of our funded activities during the period 15 February 2007 to 30 September 2012. These activities were primarily carried out at Fermilab, RHIC, and the German lab DESY. Significant advances in these experiments were carried out by members of the Colorado group and are described in detail.

  1. Final Technical Report - Center for Technology for Advanced Scientific Component Software (TASCS)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sussman, Alan

    2014-10-21

    This is a final technical report for the University of Maryland work in the SciDAC Center for Technology for Advanced Scientific Component Software (TASCS). The Maryland work focused on software tools for coupling parallel software components built using the Common Component Architecture (CCA) APIs. Those tools are based on the Maryland InterComm software framework that has been used in multiple computational science applications to build large-scale simulations of complex physical systems that employ multiple separately developed codes.

  2. Iowa Hill Pumped Storage Project Investigations - Final Technical Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hanson, David

    2016-07-01

    This Final Technical Report is a summary of the activities and outcome of the Department of Energy (DOE) Assistance Agreement DE-EE0005414 with the Sacramento Municipal Utility District (SMUD). The Assistance Agreement was created in 2012 to support investigations into the Iowa Hill Pumped-storage Project (Project), a new development that would add an additional 400 MW of capacity to SMUD’s existing 688MW Upper American River Hydroelectric Project (UARP) in the Sierra Nevada mountains east of Sacramento, California.

  3. Global characterization of copy number variants in epilepsy patients from whole genome sequencing

    PubMed Central

    Meloche, Caroline; Andrade, Danielle M.; Lafreniere, Ron G.; Gravel, Micheline; Spiegelman, Dan; Dionne-Laporte, Alexandre; Boelman, Cyrus; Hamdan, Fadi F.; Michaud, Jacques L.; Rouleau, Guy; Minassian, Berge A.; Bourque, Guillaume; Cossette, Patrick

    2018-01-01

    Epilepsy will affect nearly 3% of people at some point during their lifetime. Previous copy number variants (CNVs) studies of epilepsy have used array-based technology and were restricted to the detection of large or exonic events. In contrast, whole-genome sequencing (WGS) has the potential to more comprehensively profile CNVs but existing analytic methods suffer from limited accuracy. We show that this is in part due to the non-uniformity of read coverage, even after intra-sample normalization. To improve on this, we developed PopSV, an algorithm that uses multiple samples to control for technical variation and enables the robust detection of CNVs. Using WGS and PopSV, we performed a comprehensive characterization of CNVs in 198 individuals affected with epilepsy and 301 controls. For both large and small variants, we found an enrichment of rare exonic events in epilepsy patients, especially in genes with predicted loss-of-function intolerance. Notably, this genome-wide survey also revealed an enrichment of rare non-coding CNVs near previously known epilepsy genes. This enrichment was strongest for non-coding CNVs located within 100 Kbp of an epilepsy gene and in regions associated with changes in the gene expression, such as expression QTLs or DNase I hypersensitive sites. Finally, we report on 21 potentially damaging events that could be associated with known or new candidate epilepsy genes. Our results suggest that comprehensive sequence-based profiling of CNVs could help explain a larger fraction of epilepsy cases. PMID:29649218

  4. Renewable Fuel Standard Program (RFS1): Final Rule Additional Resources

    EPA Pesticide Factsheets

    The final rule of fuels and fuel additives: renewable fuel standard program is published on May 1, 2007 and is effective on September 1, 2007. You will find the links to this final rule and technical amendments supporting this rule.

  5. Assurance Penalty Level Analysis Final Rule TSD

    EPA Pesticide Factsheets

    This Technical Support Document (TSD) supports EPA’s determination that the final Transport Rule’s assurance provision penalty requirement provides sufficient deterrence against a state exceeding its assurance level.

  6. Final Rule for Technical Amendments to the Highway and Nonroad Diesel Regulations

    EPA Pesticide Factsheets

    This action corrects errors and omissions from the previous rules, makes minor changes to the regulations to assist entities with regulatory compliance, and makes technical amendments that resulted from discussions with various diesel stakeholders.

  7. Newborn Sequencing in Genomic Medicine and Public Health.

    PubMed

    Berg, Jonathan S; Agrawal, Pankaj B; Bailey, Donald B; Beggs, Alan H; Brenner, Steven E; Brower, Amy M; Cakici, Julie A; Ceyhan-Birsoy, Ozge; Chan, Kee; Chen, Flavia; Currier, Robert J; Dukhovny, Dmitry; Green, Robert C; Harris-Wai, Julie; Holm, Ingrid A; Iglesias, Brenda; Joseph, Galen; Kingsmore, Stephen F; Koenig, Barbara A; Kwok, Pui-Yan; Lantos, John; Leeder, Steven J; Lewis, Megan A; McGuire, Amy L; Milko, Laura V; Mooney, Sean D; Parad, Richard B; Pereira, Stacey; Petrikin, Joshua; Powell, Bradford C; Powell, Cynthia M; Puck, Jennifer M; Rehm, Heidi L; Risch, Neil; Roche, Myra; Shieh, Joseph T; Veeraraghavan, Narayanan; Watson, Michael S; Willig, Laurel; Yu, Timothy W; Urv, Tiina; Wise, Anastasia L

    2017-02-01

    The rapid development of genomic sequencing technologies has decreased the cost of genetic analysis to the extent that it seems plausible that genome-scale sequencing could have widespread availability in pediatric care. Genomic sequencing provides a powerful diagnostic modality for patients who manifest symptoms of monogenic disease and an opportunity to detect health conditions before their development. However, many technical, clinical, ethical, and societal challenges should be addressed before such technology is widely deployed in pediatric practice. This article provides an overview of the Newborn Sequencing in Genomic Medicine and Public Health Consortium, which is investigating the application of genome-scale sequencing in newborns for both diagnosis and screening. Copyright © 2017 by the American Academy of Pediatrics.

  8. Final priority. Rehabilitation Training: Job-Driven Vocational Rehabilitation Technical Assistance Center. Final priority.

    PubMed

    2014-08-19

    The Assistant Secretary for Special Education and Rehabilitative Services announces a priority under the Rehabilitation Training program to establish a Job-Driven Vocational Rehabilitation Technical Assistance Center (JDVRTAC). The Assistant Secretary may use this priority for competitions in fiscal year (FY) 2014 and later years. We take this action to focus on training in an area of national need. Specifically, this priority responds to the Presidential Memorandum to Federal agencies directing them to take action to address job-driven training for the Nation's workers. The JDVRTAC will provide technical assistance (TA) to State vocational rehabilitation (VR) agencies to help them develop for individuals with disabilities training and employment opportunities that meet the needs of today's employers.

  9. NASA's Pursuit of Low-Noise Propulsion for Low-Boom Commercial Supersonic Vehicles

    NASA Technical Reports Server (NTRS)

    Bridges, James; Brown, Clifford A.; Seidel, Jonathan A.

    2018-01-01

    Since 2006, when the Fundamental Aeronautics Program was instituted within NASA's Aeronautics Mission Directorate, there has been a Project looking at the technical barriers to commercial supersonic flight. Among the barriers is the noise produced by aircraft during landing and takeoff. Over the years that followed, research was carried out at NASA aeronautics research centers, often in collaboration with academia and industry, addressing the problem. In 2013, a high-level milestone was established, described as a Technical Challenge, with the objective of demonstrating the feasibility of a low-boom supersonic airliner that could meet current airport noise regulations. The Technical Challenge was formally called "Low Noise Propulsion for Low Boom Aircraft", and was completed in late 2016. This paper reports the technical findings from this Technical Challenge, reaching back almost 10 years to review the technologies and tools that were developed along the way. It also discusses the final aircraft configuration and propulsion systems required for a supersonic civilian aircraft to meet noise regulations using the technologies available today. Finally, the paper documents the model-scale tests that validated the acoustic performance of the study aircraft.

  10. NASA's Pursuit of Low-Noise Propulsion for Low-Boom Commercial Supersonic Vehicles

    NASA Technical Reports Server (NTRS)

    Bridges, James; Brown, Clifford A.; Seidel, Jonathan

    2018-01-01

    Since 2006, when the Fundamental Aeronautics Program was instituted within NASA's Aeronautics Mission Directorate, there has been a Project looking at the technical barriers to commercial supersonic flight. Among the barriers is the noise produced by aircraft during landing and takeoff. Over the years that followed, research was carried out at NASA aeronautics research centers, often in collaboration with academia and industry, addressing the problem. In 2013, a high-level milestone was established, described as a Technical Challenge, with the objective of demonstrating the feasibility of a low-boom supersonic airliner that could meet current airport noise regulations. The Technical Challenge was formally called a Low Noise Propulsion for Low Boom Aircraft and was completed in late 2016. This paper reports the technical findings from this Technical Challenge, reaching back almost 10 years to review the technologies and tools that were developed along the way. It also discusses the final aircraft configuration and propulsion systems required for a supersonic civilian aircraft to meet noise regulations using the technologies available today. Finally, the paper documents the model-scale tests that validated the acoustic performance of the study aircraft.

  11. Final Revisions Rule State Budgets and New Unit Set-Asides TSD

    EPA Pesticide Factsheets

    This technical support document shows the underlying data and calculations used to quantify the state budget revisions and new unit set-aside revisions made in the final revisions rule, as well as those revisions included in the direct final revisions rule

  12. 77 FR 37284 - Technical Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-21

    ... DEPARTMENT OF LABOR Office of Workers' Compensation Programs 20 CFR Parts 701, 702, 703, 725, and 726 RIN 1240-AA05 Technical Amendments AGENCY: Office of Workers' Compensation Programs, Labor. ACTION: Final rule. SUMMARY: The Office of Workers' Compensation Programs is making [[Page 37285

  13. Vehicle infrastructure integration proof of concept : technical description--vehicle : final report

    DOT National Transportation Integrated Search

    2009-05-19

    This report provides the technical description of the VII system developed for the Cooperative Agreement VII Program between the USDOT and the VII Consortium. The basic architectural elements are summarized and detailed descriptions of the hardware a...

  14. NetView technical research

    NASA Technical Reports Server (NTRS)

    1993-01-01

    This is the Final Technical Report for the NetView Technical Research task. This report is prepared in accordance with Contract Data Requirements List (CDRL) item A002. NetView assistance was provided and details are presented under the following headings: NetView Management Systems (NMS) project tasks; WBAFB IBM 3090; WPAFB AMDAHL; WPAFB IBM 3084; Hill AFB; McClellan AFB AMDAHL; McClellan AFB IBM 3090; and Warner-Robins AFB.

  15. Inventory of greenhouse gas emissions from on-road vehicles in Midwestern USA States and integrated approach to achieving environmental sustainability in transportation : USDOT Region V Regional University Transportation Center final report : technical su

    DOT National Transportation Integrated Search

    2016-12-29

    Two project objectives one technical and one educational- were laid out in this project. The technical objective was to assess current inventory of greenhouse gases (GHG) in the six Midwestern states of the nation and to estimate improvements as ...

  16. Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding.

    PubMed

    Lan, Freeman; Demaree, Benjamin; Ahmed, Noorsher; Abate, Adam R

    2017-07-01

    The application of single-cell genome sequencing to large cell populations has been hindered by technical challenges in isolating single cells during genome preparation. Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single cells, followed by Illumina sequencing of pooled DNA. We demonstrate ultra-high-throughput sequencing of >50,000 cells per run in a synthetic community of Gram-negative and Gram-positive bacteria and fungi. The sequenced genomes can be sorted in silico based on characteristic sequences. We use this approach to analyze the distributions of antibiotic-resistance genes, virulence factors, and phage sequences in microbial communities from an environmental sample. The ability to routinely sequence large populations of single cells will enable the de-convolution of genetic heterogeneity in diverse cell populations.

  17. Emissions Inventory Final Rule TSD

    EPA Pesticide Factsheets

    This technical support document (TSD) provides the details of emissions data processing done in support of EPA's final rulemaking effort for the Federal Transport Rule, now known as the Cross-State Air Pollution Rule.

  18. Aligning Career and Technical Education with High-Wage and High-Demand Occupations in Tennessee. Issues & Answers. REL 2011-No. 111

    ERIC Educational Resources Information Center

    Mokher, Christine

    2011-01-01

    This study examines the availability of career and technical education program areas in Tennessee high schools, concentrations (a three-or-more credit sequence in a program area) completed by 2007/08 high school graduates, and how these concentrations align with jobs in the labor market. It looks at how these outcomes differ, statewide and by…

  19. Evaluation of Markov-Decision Model for Instructional Sequence Optimization. Semi-Annual Technical Report for the period 1 July-31 December 1975. Technical Report No. 76.

    ERIC Educational Resources Information Center

    Wollmer, Richard D.; Bond, Nicholas A.

    Two computer-assisted instruction programs were written in electronics and trigonometry to test the Wollmer Markov Model for optimizing hierarchial learning; calibration samples totalling 110 students completed these programs. Since the model postulated that transfer effects would be a function of the amount of practice, half of the students were…

  20. Transportation Network Data Requirements for Assessing Criticality for Resiliency and Adaptation Planning

    DOT National Transportation Integrated Search

    2017-11-01

    This report is one of two NCST Research Report documents produced as part of a project to advance the technical modeling tools for resiliency and adaptation planning, especially those used for criticality rankings. The official final technical report...

  1. Comment Deadlines Established Regarding the LightSquared Technical Working Group Report

    DOT National Transportation Integrated Search

    2011-06-30

    On June 30, 2011, LightSquared Subsidiary LLC (LightSquared) submitted a final report of the : technical working group co-chaired by LightSquared and the United States Global Positioning System : (GPS) Industry Council (USGIC)1 and organized in respo...

  2. Technical report on prototype intelligent network flow optimization (INFLO) dynamic speed harmonization and queue warning.

    DOT National Transportation Integrated Search

    2015-06-01

    This Technical Report on Prototype Intelligent Network Flow Optimization (INFLO) Dynamic Speed Harmonization and Queue Warning is the final report for the project. It describes the prototyping, acceptance testing and small-scale demonstration of the ...

  3. NUSC Technical Publications Guide.

    DTIC Science & Technology

    1985-05-01

    Facility personnel especially that of A. Castelluzzo, E. Deland, J. Gesel , and E. Szlosek (all of Code 4343). Reviewed and Approved: 14 July 1980 D...their technical content and format. Review and approve the manual outline, the review manuscript, and the final camera - reproducible copy. Conduct in

  4. Final Technical Report - Use of Systems Biology Approaches to Develop Advanced Biofuel-Synthesizing Cyanobacterial Strains

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pakrasi, Himadri

    The overall objective of this project was to use a systems biology approach to evaluate the potentials of a number of cyanobacterial strains for photobiological production of advanced biofuels and/or their chemical precursors. Cyanobacteria are oxygen evolving photosynthetic prokaryotes. Among them, certain unicellular species such as Cyanothece can also fix N 2, a process that is exquisitely sensitive to oxygen. To accommodate such incompatible processes in a single cell, Cyanothece produces oxygen during the day, and creates an O 2-limited intracellular environment during the night to perform O 2-sensitive processes such as N 2-fixation. Thus, Cyanothece cells are natural bioreactorsmore » for the storage of captured solar energy with subsequent utilization at a different time during a diurnal cycle. Our studies include the identification of a novel, fast-growing, mixotrophic, transformable cyanobacterium. This strain has been sequenced and will be made available to the community. In addition, we have developed genome-scale models for a family of cyanobacteria to assess their metabolic repertoire. Furthermore, we developed a method for rapid construction of metabolic models using multiple annotation sources and a metabolic model of a related organism. This method will allow rapid annotation and screening of potential phenotypes based on the newly available genome sequences of many organisms.« less

  5. Precise Sequential DNA Ligation on A Solid Substrate: Solid-Based Rapid Sequential Ligation of Multiple DNA Molecules

    PubMed Central

    Takita, Eiji; Kohda, Katsunori; Tomatsu, Hajime; Hanano, Shigeru; Moriya, Kanami; Hosouchi, Tsutomu; Sakurai, Nozomu; Suzuki, Hideyuki; Shinmyo, Atsuhiko; Shibata, Daisuke

    2013-01-01

    Ligation, the joining of DNA fragments, is a fundamental procedure in molecular cloning and is indispensable to the production of genetically modified organisms that can be used for basic research, the applied biosciences, or both. Given that many genes cooperate in various pathways, incorporating multiple gene cassettes in tandem in a transgenic DNA construct for the purpose of genetic modification is often necessary when generating organisms that produce multiple foreign gene products. Here, we describe a novel method, designated PRESSO (precise sequential DNA ligation on a solid substrate), for the tandem ligation of multiple DNA fragments. We amplified donor DNA fragments with non-palindromic ends, and ligated the fragment to acceptor DNA fragments on solid beads. After the final donor DNA fragments, which included vector sequences, were joined to the construct that contained the array of fragments, the ligation product (the construct) was thereby released from the beads via digestion with a rare-cut meganuclease; the freed linear construct was circularized via an intra-molecular ligation. PRESSO allowed us to rapidly and efficiently join multiple genes in an optimized order and orientation. This method can overcome many technical challenges in functional genomics during the post-sequencing generation. PMID:23897972

  6. DNAseq Workflow in a Diagnostic Context and an Example of a User Friendly Implementation.

    PubMed

    Wolf, Beat; Kuonen, Pierre; Dandekar, Thomas; Atlan, David

    2015-01-01

    Over recent years next generation sequencing (NGS) technologies evolved from costly tools used by very few, to a much more accessible and economically viable technology. Through this recently gained popularity, its use-cases expanded from research environments into clinical settings. But the technical know-how and infrastructure required to analyze the data remain an obstacle for a wider adoption of this technology, especially in smaller laboratories. We present GensearchNGS, a commercial DNAseq software suite distributed by Phenosystems SA. The focus of GensearchNGS is the optimal usage of already existing infrastructure, while keeping its use simple. This is achieved through the integration of existing tools in a comprehensive software environment, as well as custom algorithms developed with the restrictions of limited infrastructures in mind. This includes the possibility to connect multiple computers to speed up computing intensive parts of the analysis such as sequence alignments. We present a typical DNAseq workflow for NGS data analysis and the approach GensearchNGS takes to implement it. The presented workflow goes from raw data quality control to the final variant report. This includes features such as gene panels and the integration of online databases, like Ensembl for annotations or Cafe Variome for variant sharing.

  7. Cross-State Air Pollution Rule Update Allowance Allocation Final Rule TSD

    EPA Pesticide Factsheets

    This Technical Support Document (TSD) provides information that supports EPA’s determination of unit-level allocations for existing and new units under the final Cross-State Air Pollution Rule Update.

  8. Standard Free Droplet Digital Polymerase Chain Reaction as a New Tool for the Quality Control of High-Capacity Adenoviral Vectors in Small-Scale Preparations

    PubMed Central

    Boehme, Philip; Stellberger, Thorsten; Solanki, Manish; Zhang, Wenli; Schulz, Eric; Bergmann, Thorsten; Liu, Jing; Doerner, Johannes; Baiker, Armin E.

    2015-01-01

    Abstract High-capacity adenoviral vectors (HCAdVs) are promising tools for gene therapy as well as for genetic engineering. However, one limitation of the HCAdV vector system is the complex, time-consuming, and labor-intensive production process and the following quality control procedure. Since HCAdVs are deleted for all viral coding sequences, a helper virus (HV) is needed in the production process to provide the sequences for all viral proteins in trans. For the purification procedure of HCAdV, cesium chloride density gradient centrifugation is usually performed followed by buffer exchange using dialysis or comparable methods. However, performing these steps is technically difficult, potentially error-prone, and not scalable. Here, we establish a new protocol for small-scale production of HCAdV based on commercially available adenovirus purification systems and a standard method for the quality control of final HCAdV preparations. For titration of final vector preparations, we established a droplet digital polymerase chain reaction (ddPCR) that uses a standard free-end-point PCR in small droplets of defined volume. By using different probes, this method is capable of detecting and quantifying HCAdV and HV in one reaction independent of reference material, rendering this method attractive for accurately comparing viral titers between different laboratories. In summary, we demonstrate that it is possible to produce HCAdV in a small scale of sufficient quality and quantity to perform experiments in cell culture, and we established a reliable protocol for vector titration based on ddPCR. Our method significantly reduces time and required equipment to perform HCAdV production. In the future the ddPCR technology could be advantageous for titration of other viral vectors commonly used in gene therapy. PMID:25640117

  9. NASA Institute for Advanced Concepts

    NASA Technical Reports Server (NTRS)

    Cassanova, Robert A.

    1999-01-01

    The purpose of NASA Institute for Advanced Concepts (NIAC) is to provide an independent, open forum for the external analysis and definition of space and aeronautics advanced concepts to complement the advanced concepts activities conducted within the NASA Enterprises. The NIAC will issue Calls for Proposals during each year of operation and will select revolutionary advanced concepts for grant or contract awards through a peer review process. Final selection of awards will be with the concurrence of NASA's Chief Technologist. The operation of the NIAC is reviewed biannually by the NIAC Science, Exploration and Technology Council (NSETC) whose members are drawn from the senior levels of industry and universities. The process of defining the technical scope of the initial Call for Proposals was begun with the NIAC "Grand Challenges" workshop conducted on May 21-22, 1998 in Columbia, Maryland. These "Grand Challenges" resulting from this workshop became the essence of the technical scope for the first Phase I Call for Proposals which was released on June 19, 1998 with a due date of July 31, 1998. The first Phase I Call for Proposals attracted 119 proposals. After a thorough peer review, prioritization by NIAC and technical concurrence by NASA, sixteen subgrants were awarded. The second Phase I Call for Proposals was released on November 23, 1998 with a due date of January 31, 1999. Sixty-three (63) proposals were received in response to this Call. On December 2-3, 1998, the NSETC met to review the progress and future plans of the NIAC. The next NSETC meeting is scheduled for August 5-6, 1999. The first Phase II Call for Proposals was released to the current Phase I grantees on February 3,1999 with a due date of May 31, 1999. Plans for the second year of the contract include a continuation of the sequence of Phase I and Phase II Calls for Proposals and hosting the first NIAC Annual Meeting and USRA/NIAC Technical Symposium in NASA HQ.

  10. Guidelines for producing training films and videos.

    PubMed

    Harper, P B

    1991-01-01

    Drawing from experience in producing a film on the surgical procedure of female sterilization, 4 guidelines to technical film production for training purposes are presented and discussed in this paper. In order of presentation in the text, the paper 1st encourages identifying and securing a technical expert, then clearly identifying steps of the technical procedure, involving trainees and trainers in the production process, and working with experienced producers, scriptwriters, and crew members. Returning to the 1st guideline, the technical advisor will have a central presence during all photography and editing, and ideally should not have any personal investment in the procedure being shown. Prior to script finalization and sorting, research is urged to ensure concrete procedural steps. Printed materials, slides, interviews of experienced clinicians, procedure observation, and test videotape shooting may be called upon and employed as parts of the research phase. Trainees should participate during preliminary research, script development, and pretesting of early film versions, their suggestions for change incorporated where appropriate in the final version. On the final point of securing experienced workers, country nationals sensitive to relevant cultural and background dynamics should be included in the team. The special concerns of airport security regulation and customs requirements knowledge are essential, as well as their attention to assuring adequate on-site electricity for camera equipment.

  11. Northeast Corridor Improvement Project Electrification - New Haven, CT to Boston, MA : Final Environmental Statement/Report : v. 2. Technical Studies

    DOT National Transportation Integrated Search

    1994-10-31

    This document is the final environmental impact statement and final environmental impact report (FEIS/R) on the proposal by the National Railroad Passenger Corporation (Amtrak) to complete the electrification of the Northeast Corridor main line by ex...

  12. Health care fraud and abuse data collection program: technical revisions to Healthcare Integrity and Protection Data Bank data collection activities. Final rule.

    PubMed

    2004-09-21

    The rule finalizes technical changes to the Healthcare Integrity and Protection Data Bank (HIPDB) data collection reporting requirements by clarifying the types of personal numeric identifiers that may be reported to the data bank in connection with adverse actions. The rule clarifies that in lieu of a Social Security Number (SSN), an individual taxpayer identification number (ITIN) may be reported to the data bank when, in those limited situations, an individual does not have an SSN.

  13. Recovery Act-SmartGrid regional demonstration transmission and distribution (T&D) Infrastructure

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hedges, Edward T.

    This document represents the Final Technical Report for the Kansas City Power & Light Company (KCP&L) Green Impact Zone SmartGrid Demonstration Project (SGDP). The KCP&L project is partially funded by Department of Energy (DOE) Regional Smart Grid Demonstration Project cooperative agreement DE-OE0000221 in the Transmission and Distribution Infrastructure application area. This Final Technical Report summarizes the KCP&L SGDP as of April 30, 2015 and includes summaries of the project design, implementation, operations, and analysis performed as of that date.

  14. Final Technical Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sobecky, Patricia A; Taillefert, Martial

    This final technical report describes results and findings from a research project to examine the role of microbial phosphohydrolase enzymes in naturally occurring subsurface microorganisms for the purpose of promoting the immobilization of the radionuclide uranium through the production of insoluble uranium phosphate minerals. The research project investigated the microbial mechanisms and the physical and chemical processes promoting uranium biomineralization and sequestration in oxygenated subsurface soils. Uranium biomineralization under aerobic conditions can provide a secondary biobarrier strategy to immobilize radionuclides should the metal precipitates formed by microbial dissimilatory mechanisms remobilize due to a change in redox state.

  15. Waveguide Studies for Fiber Optics and Optical Signal Processing Applications.

    DTIC Science & Technology

    1980-04-01

    AO-A086 115 UNI!VERtSIT? OF SOUTIUR CALEPCRNA LOS AMUSS / 5 WAVGUIDE STUIES15 FOR FEB53 OpTECS AND OpTICAL SEOSA.o P /0Ksu-y "/6 UNLSIIDAPR N0 E...SAMUE Flola-??-c-sa UNCASZFIORAC-M-8042 U Final Technical Report (1 1April 1950 L V ~ WAVEGUIDE STUDIES FOR FIBER OPTICS AND OPTICAL SIGNAL PROCESSING...and Subtitle) 081 6&4𔃾JODO )EI YAVECUIDESTUDIES FOR JIBER OPTICS ANDL 7 Final ,T/echnical epoErt, OPTICAL SI’tNAL PROCESSING APPLICATIONS.4 11 Se 77

  16. A Unique Sequence of Financial Accounting Courses Featuring Team Teaching, Linked Courses, Challenging Assignments, and Instruments for Evaluation and Assessment

    ERIC Educational Resources Information Center

    Lundblad, Heidemarie; Wilson, Barbara A.

    2008-01-01

    The Department of Accounting at California State University Northridge (CSUN) has developed a unique sequence of courses designed to ensure that accounting students are trained not only in technical accounting, but also acquire critical thinking, research and communication skills. The courses have proven effective and have embedded assessment…

  17. Meta-analysis of RNA-Seq data across cohorts in a multi-season feed efficiency study of crossbred beef steers accounts for biological and technical variability within season

    USDA-ARS?s Scientific Manuscript database

    High-throughput sequencing is often used for studies of the transcriptome, particularly for comparisons between experimental conditions. Due to sequencing costs, a limited number of biological replicates are typically considered in such experiments, leading to low detection power for differential ex...

  18. SELECTIVE DISSEMINATION OF INFORMATION (SDI). VOLUME I, PILOT TEST AT U.S. ARMY NATICK LABORATORIES. FINAL REPORT. ARMY TECHNICAL LIBRARY IMPROVEMENT STUDIES (ATLIS), REPORT NO. 15.

    ERIC Educational Resources Information Center

    BIVONA, WILLIAM A.

    THIS VOLUME PRESENTS THE RESULTS OF A NINE-MONTH TEST OF A PROTOTYPE SELECTIVE DISSEMINATION OF INFORMATION (SDI) SYSTEM DEVELOPED FOR THE ARMY TECHNICAL LIBRARIES. DURING THE PILOT TEST ONE THOUSAND DOCUMENTS WERE CATALOGED, INDEXED, AND DISSEMINATED TO TWENTY-FIVE SCIENTIFIC AND TECHNICAL PERSONNEL. MATCHING OF THE INTEREST PROFILES OF THESE…

  19. Ceramic High Efficiency Particulate Air (HEPA) Filter Final Report CRADA No. TC02160.0

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mitchell, M.; Bergman, W.

    2017-08-25

    The technical objective of this project was to develop a ceramic HEPA filter technology, by initially producing and testing coupon ceramics, small scale prototypes, and full scale prototype HEPA filters, and to address relevant manufacturing and commercialization technical issues.

  20. 7 CFR 614.6 - Agency records and decision notices.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... notifies participants of the agency's preliminary and final technical determinations and program decisions... decision notice within 10 working days of rendering a technical determination or program decision. In lieu of certified mail, NRCS may hand deliver notices to participants with written acknowledgment of...

  1. ECOS E-MATRIX Methane and Volatile Organic Carbon (VOC) Emissions Best Practices Database

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Parisien, Lia

    2016-01-31

    This final scientific/technical report on the ECOS e-MATRIX Methane and Volatile Organic Carbon (VOC) Emissions Best Practices Database provides a disclaimer and acknowledgement, table of contents, executive summary, description of project activities, and briefing/technical presentation link.

  2. 77 FR 18914 - National Motor Vehicle Title Information System (NMVTIS): Technical Corrections

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-29

    ... 1121-AA79 National Motor Vehicle Title Information System (NMVTIS): Technical Corrections AGENCY... (OJP) is promulgating this direct final rule for its National Motor Vehicle Title Information System... INFORMATION CONTACT paragraph. II. Background The National Motor Vehicle Title Information System was...

  3. Intelligent transportation system (ITS) study for the Buffalo and Niagara Falls metropolitan area, Erie and Niagara Counties, New York : final report, technical summary

    DOT National Transportation Integrated Search

    1997-06-18

    This document provides a technical summary for the seven working papers prepared for the New York State Department of Transportation (NYSDOT) Buffalo and Niagara Falls Intelligent Transportation System (ITS) Study.

  4. 77 FR 8095 - Technical Corrections to Commission Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-14

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission 18 CFR Part 2 [Docket No. RM11-30-000; Order No. 756] Technical Corrections to Commission Regulations Issued February 8, 2012. AGENCY: Federal Energy Regulatory Commission, DOE. ACTION: Final rule: correcting amendment. SUMMARY: This document adds...

  5. 78 FR 13543 - Defense Federal Acquisition Regulation Supplement; Technical Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Parts 201, 204, 215, 225, 227, 242, 245, and 252 Defense Federal Acquisition Regulation Supplement; Technical Amendments AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Final rule. SUMMARY: DoD is...

  6. Controversy and debate on clinical genomics sequencing-paper 1: genomics is not exceptional: rigorous evaluations are necessary for clinical applications of genomic sequencing.

    PubMed

    Wilson, Brenda J; Miller, Fiona Alice; Rousseau, François

    2017-12-01

    Next generation genomic sequencing (NGS) technologies-whole genome and whole exome sequencing-are now cheap enough to be within the grasp of many health care organizations. To many, NGS is symbolic of cutting edge health care, offering the promise of "precision" and "personalized" medicine. Historically, research and clinical application has been a two-way street in clinical genetics: research often driven directly by the desire to understand and try to solve immediate clinical problems affecting real, identifiable patients and families, accompanied by a low threshold of willingness to apply research-driven interventions without resort to formal empirical evaluations. However, NGS technologies are not simple substitutes for older technologies and need careful evaluation for use as screening, diagnostic, or prognostic tools. We have concerns across three areas. First, at the moment, analytic validity is unknown because technical platforms are not yet stable, laboratory quality assurance programs are in their infancy, and data interpretation capabilities are badly underdeveloped. Second, clinical validity of genomic findings for patient populations without pre-existing high genetic risk is doubtful, as most clinical experience with NGS technologies relates to patients with a high prior likelihood of a genetic etiology. Finally, we are concerned that proponents argue not only for clinically driven approaches to assessing a patient's genome, but also for seeking out variants associated with unrelated conditions or susceptibilities-so-called "secondary targets"-this is screening on a genomic scale. We argue that clinical uses of genomic sequencing should remain limited to specialist and research settings, that screening for secondary findings in clinical testing should be limited to the maximum extent possible, and that the benefits, harms, and economic implications of their routine use be systematically evaluated. All stakeholders have a responsibility to ensure that patients receive effective, safe health care, in an economically sustainable health care system. There should be no exception for genome-based interventions. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. Aligning Career and Technical Education with High-Wage and High-Demand Occupations in Tennessee. Summary. Issues & Answers. REL 2011-No. 111

    ERIC Educational Resources Information Center

    Mokher, Christine

    2011-01-01

    This study examines the availability of career and technical education program areas in Tennessee high schools, concentrations (a three-or-more credit sequence in a program area) completed by 2007/08 high school graduates, and how these concentrations align with jobs in the labor market. It looks at how these outcomes differ, statewide and by…

  8. Pan-cancer analysis reveals technical artifacts in TCGA germline variant calls.

    PubMed

    Buckley, Alexandra R; Standish, Kristopher A; Bhutani, Kunal; Ideker, Trey; Lasken, Roger S; Carter, Hannah; Harismendy, Olivier; Schork, Nicholas J

    2017-06-12

    Cancer research to date has largely focused on somatically acquired genetic aberrations. In contrast, the degree to which germline, or inherited, variation contributes to tumorigenesis remains unclear, possibly due to a lack of accessible germline variant data. Here we called germline variants on 9618 cases from The Cancer Genome Atlas (TCGA) database representing 31 cancer types. We identified batch effects affecting loss of function (LOF) variant calls that can be traced back to differences in the way the sequence data were generated both within and across cancer types. Overall, LOF indel calls were more sensitive to technical artifacts than LOF Single Nucleotide Variant (SNV) calls. In particular, whole genome amplification of DNA prior to sequencing led to an artificially increased burden of LOF indel calls, which confounded association analyses relating germline variants to tumor type despite stringent indel filtering strategies. The samples affected by these technical artifacts include all acute myeloid leukemia and practically all ovarian cancer samples. We demonstrate how technical artifacts induced by whole genome amplification of DNA can lead to false positive germline-tumor type associations and suggest TCGA whole genome amplified samples be used with caution. This study draws attention to the need to be sensitive to problems associated with a lack of uniformity in data generation in TCGA data.

  9. Alternatives for Jet Engine Control. Volume 1: Modelling and Control Design with Jet Engine Data

    NASA Technical Reports Server (NTRS)

    Sain, M. K.

    1985-01-01

    This document compiles a comprehensive list of publications supported by, or related to, National Aeronautics and Space Administration Grant NSG-3048, entitled "Alternatives for Jet Engine Control". Dr. Kurt Seldner was the original Technical Officer for the grant, at Lewis Research Center. Dr. Bruce Lehtinen was the final Technical Officer. At the University of Notre Dame, Drs. Michael K. Sain and R. Jeffrey Leake were the original Project Directors, with Dr. Sain becoming the final Project Director. Publications cover work over a ten-year period. The Final Report is divided into two parts. Volume i, "Modelling and Control Design with Jet Engine Data", follows in this report. Volume 2, "Modelling and Control Design with Tensors", has been bound separately.

  10. 48 CFR 252.235-7011 - Final scientific or technical report.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... technical report. 252.235-7011 Section 252.235-7011 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... of the report; and (c) For submission of reports in other than paper copy, contact the Defense...

  11. 48 CFR 252.235-7011 - Final scientific or technical report.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... technical report. 252.235-7011 Section 252.235-7011 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... of the report; and (c) For submission of reports in other than paper copy, contact the Defense...

  12. 48 CFR 252.235-7011 - Final scientific or technical report.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... technical report. 252.235-7011 Section 252.235-7011 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... of the report; and (c) For submission of reports in other than paper copy, contact the Defense...

  13. 48 CFR 252.235-7011 - Final scientific or technical report.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... technical report. 252.235-7011 Section 252.235-7011 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... of the report; and (c) For submission of reports in other than paper copy, contact the Defense...

  14. 48 CFR 252.235-7011 - Final scientific or technical report.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... technical report. 252.235-7011 Section 252.235-7011 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... of the report; and (c) For submission of reports in other than paper copy, contact the Defense...

  15. Vocational Outreach Involving Community Exchange (VOICE). Final Report.

    ERIC Educational Resources Information Center

    Huckabee, Johnni

    A Jonesboro, Arkansas project was designed and implemented to increase the awareness of vocational education, increase community support and involvement in vocational education, and establish improved communication in the vocational-technical education field. Interaction between the vocational-technical school and the local school community was a…

  16. The History of Radio Astronomy and the National Radio Astronomy Observatory: Evolution Toward Big Science

    NASA Astrophysics Data System (ADS)

    Malphrus, Benjamin Kevin

    1990-01-01

    The purpose of this study is to examine the sequence of events that led to the establishment of the NRAO, the construction and development of instrumentation and the contributions and discovery events and to relate the significance of these events to the evolution of the sciences of radio astronomy and cosmology. After an overview of the resources, a brief discussion of the early days of the science is given to set the stage for an examination of events that led to the establishment of the NRAO. The developmental and construction phases of the major instruments including the 85-foot Tatel telescope, the 300-foot telescope, the 140-foot telescope, and the Green Bank lnterferometer are examined. The technical evolution of these instruments is traced and their relevance to scientific programs and discovery events is discussed. The history is told in narrative format that is interspersed with technical and scientific explanations. Through the use of original data technical and scientific information of historical concern is provided to elucidate major developments and events. An interpretive discussion of selected programs, events and technological developments that epitomize the contributions of the NRAO to the science of radio astronomy is provided. Scientific programs conducted with the NRAO instruments that were significant to galactic and extragalactic astronomy are presented. NRAO research programs presented include continuum and source surveys, mapping, a high precision verification of general relativity, and SETI programs. Cosmic phenomena investigated in these programs include galactic and extragalactic HI and HII, emission nebula, supernova remnants, cosmic masers, giant molecular clouds, radio stars, normal and radio galaxies, and quasars. Modern NRAO instruments including the VLA and VLBA and their scientific programs are presented in the final chapter as well as plans for future NRAO instruments such as the GBT.

  17. Advancements in the use of speleothems as climate archives

    NASA Astrophysics Data System (ADS)

    Wong, Corinne I.; Breecker, Daniel O.

    2015-11-01

    Speleothems have become a cornerstone of the approach to better understanding Earth's climatic teleconnections due to their precise absolute chronologies, their continuous or semicontinuous deposition and their global terrestrial distribution. We review the last decade of speleothem-related research, building off a similar review by McDermott (2004), in three themes - i) investigation of global teleconnections using speleothem-based climate reconstructions, ii) refinement of climate interpretations from speleothem proxies through cave monitoring, and iii) novel, technical methods of speleothem-based climate reconstructions. Speleothem records have enabled critical insight into the response of global hydroclimate to large climate changes. This includes the relevant forcings and sequence of climatic responses involved in glacial terminations and recognition of a global monsoon response to climate changes on orbital and millennial time scales. We review advancements in understanding of the processes that control speleothem δ13C values and introduce the idea of a direct atmospheric pCO2 influence. We discuss progress in understanding kinetic isotope fractionation, which, with further advances, may help quantify paleoclimate changes despite non-equilibrium formation of speleothems. This feeds into the potential of proxy system modeling to consider climatic, hydrological and biogeochemical processes with the objective of quantitatively interpreting speleothem proxies. Finally, we provide an overview of emerging speleothem proxies and novel approaches using existing proxies. Most recently, technical advancements made in the measurement of fluid inclusions are now yielding reliable determinations of paleotemperatures.

  18. King County Metro Transit Hybrid Articulated Buses: Final Evaluation Results

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Chandler, K.; Walkowicz, K.

    2006-12-01

    Final technical report compares and evaluates new diesel and diesel hybrid-electric articulated buses operated as part of the King County Metro Transit (KC Metro) fleet in Seattle, Washington. The evaluation lasted 12 months.

  19. 78 FR 1143 - Explosive Siting Requirements; Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-08

    ... launch site operators in site planning for the storage and handling of energetic liquids and explosives...: For technical questions concerning this final rule, contact Yvonne Tran, Commercial Space... this final rule, contact Laura Montgomery, AGC 200, [[Page 1144

  20. Final June Revisions Rule State Budgets and New Unit Set-Asides TSD

    EPA Pesticide Factsheets

    This technical support document (TSD) for the final revisions to the Transport Rule shows the underlying data and calculations used to quantify the state budget revisions and new unit set-aside revisions.

  1. Joint electrical engineering/physics course sequence for optics fundamentals and design

    NASA Astrophysics Data System (ADS)

    Magnusson, Robert; Maldonado, Theresa A.; Black, Truman D.

    2000-06-01

    Optics is a key technology in a broad range of engineering and science applications of high national priority. Engineers and scientists with a sound background in this field are needed to preserve technical leadership and to establish new directions of research and development. To meet this educational need, a joint Electrical Engineering/Physics optics course sequence was created as PHYS 3445 Fundamentals of Optics and EE 4444 Optical Systems Design, both with a laboratory component. The objectives are to educate EE and Physics undergraduate students in the fundamentals of optics; in interdisciplinary problem solving; in design and analysis; in handling optical components; and in skills such as communications and team cooperation. Written technical reports in professional format are required, formal presentations are given, and participation in paper design contests is encouraged.

  2. Department of Defense Access to Intellectual Property for Weapon Systems Sustainment

    DTIC Science & Technology

    2017-05-01

    and acquiring technical data rights …  The cost benefit analysis of including a priced contract option for the future delivery of technical data...entail in terms of cost and benefits , while one of the activities to be finalized is the contract-specific technical data elements.66...Virginia 22311-1882 May 2017 Approved for public release; distribution is unlimited. IDA Paper P-8266 Log: H 17-000030 About This Publication This

  3. 10 CFR 51.92 - Supplement to the final environmental impact statement.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... changes in the proposed action that are relevant to environmental concerns; or (2) There are new and..., technical, and other benefits and costs of the proposed action, to the extent that the final environmental... costs; (5) Include an analysis of other energy alternatives, to the extent that the final environmental...

  4. Dissemination of Continuing Education Materials Via Television Delivery Systems. Final Technical Report and Final Report.

    ERIC Educational Resources Information Center

    Munushian, Jack

    In 1972, the University of Southern California School of Engineering established a 4-channel interactive instructional television network. It was designed to allow employees of participating industries to take regular university science and engineering courses and special continuing education courses at or near their work locations. Final progress…

  5. The role of non-technical skills in surgery

    PubMed Central

    Agha, Riaz A.; Fowler, Alexander J.; Sevdalis, Nick

    2015-01-01

    Non-technical skills are of increasing importance in surgery and surgical training. A traditional focus on technical skills acquisition and competence is no longer enough for the delivery of a modern, safe surgical practice. This review discusses the importance of non-technical skills and the values that underpin successful modern surgical practice. This narrative review used a number of sources including written and online, there was no specific search strategy of defined databases. Modern surgical practice requires; technical and non-technical skills, evidence-based practice, an emphasis on lifelong learning, monitoring of outcomes and a supportive institutional and health service framework. Finally these requirements need to be combined with a number of personal and professional values including integrity, professionalism and compassionate, patient-centred care. PMID:26904193

  6. Exploiting EST databases for the development and characterisation of 3425 gene-tagged CISP markers in biofuel crop sugarcane and their transferability in cereals and orphan tropical grasses.

    PubMed

    Chandra, Amaresh; Jain, Radha; Solomon, Sushil; Shrivastava, Shiksha; Roy, Ajoy K

    2013-02-04

    Sugarcane is an important cash crop, providing 70% of the global raw sugar as well as raw material for biofuel production. Genetic analysis is hindered in sugarcane because of its large and complex polyploid genome and lack of sufficiently informative gene-tagged markers. Modern genomics has produced large amount of ESTs, which can be exploited to develop molecular markers based on comparative analysis with EST datasets of related crops and whole rice genome sequence, and accentuate their cross-technical functionality in orphan crops like tropical grasses. Utilising 246,180 Saccharum officinarum EST sequences vis-à-vis its comparative analysis with ESTs of sorghum and barley and the whole rice genome sequence, we have developed 3425 novel gene-tagged markers - namely, conserved-intron scanning primers (CISP) - using the web program GeMprospector. Rice orthologue annotation results indicated homology of 1096 sequences with expressed proteins, 491 with hypothetical proteins. The remaining 1838 were miscellaneous in nature. A total of 367 primer-pairs were tested in diverse panel of samples. The data indicate amplification of 41% polymorphic bands leading to 0.52 PIC and 3.50 MI with a set of sugarcane varieties and Saccharum species. In addition, a moderate technical functionality of a set of such markers with orphan tropical grasses (22%) and fodder cum cereal oat (33%) is observed. Developed gene-tagged CISP markers exhibited considerable technical functionality with varieties of sugarcane and unexplored species of tropical grasses. These markers would thus be particularly useful in identifying the economical traits in sugarcane and developing conservation strategies for orphan tropical grasses.

  7. 75 FR 16345 - Administrative Practices and Procedures; Good Guidance Practices; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-01

    .... FDA-1999-N-3539] (formerly Docket No. 1999N-4783) Administrative Practices and Procedures; Good Guidance Practices; Technical Amendment AGENCY: Food and Drug Administration, HHS. ACTION: Final rule... Subjects in 21 CFR Part 10 Administrative practice and procedure, News media. 0 Therefore, under the...

  8. Final report : for the period of December 1999 through November 30, 2000 : Florida Transit Training Program (1999/2000) : Florida Technical Assistance Program (1999/2000)

    DOT National Transportation Integrated Search

    2000-01-01

    The following progress report is intended to highlight the significant activities of the Florida Transit Training Program and Florida Technical Assistant Program. The following progress report is intended to highlight the significant activities of th...

  9. 78 FR 34264 - Technical Corrections to the HIPAA Privacy, Security, and Enforcement Rules

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-07

    ...-AA03 Technical Corrections to the HIPAA Privacy, Security, and Enforcement Rules AGENCY: Office for... corrections address certain inadvertent errors and omissions in the HIPAA Privacy, Security, and Enforcement... (HHS or ``the Department'') published a final rule to implement changes to the HIPAA Privacy, Security...

  10. 76 FR 11414 - Defense Federal Acquisition Regulation Supplement; Independent Research and Development Technical...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-02

    ... Acquisition Regulation Supplement; Independent Research and Development Technical Descriptions (DFARS Case... (DFARS) to require contractors to report independent research and development (IR&D) projects generating... the address shown below on or before May 2, 2011, to be considered in the formation of the final rule...

  11. Professional Personnel Evaluation System: 1983-84 Final Technical Report.

    ERIC Educational Resources Information Center

    Doss, David A.

    This technical report summarizes the evaluation ratings given to professionals on probation or up for contract renewal in the Austin Independent School District. Graphs, tables, and rankings are presented for each specified population: all district teachers combined; teachers by school level (elementary, junior high, senior high); teachers by…

  12. High School Graduation Minimum Competency Requirements. Final Technical Report.

    ERIC Educational Resources Information Center

    Austin Independent School District, TX. Office of Research and Evaluation.

    This technical report details the testing results and analyses supporting the evaluation findings related to the Austin (Texas) Independent School District (AISD) minimum competency graduation requirements. The graduation competency status of all AISD students in grades 8 to 12 are documented. The report provides additional information on the data…

  13. 76 FR 16531 - Technical Correction for Neurological Listing Cross-Reference

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-24

    ... 1-800-325-0778, or visit our Internet site, Social Security Online, at http://www.socialsecurity.gov... SOCIAL SECURITY ADMINISTRATION 20 CFR Part 404 [Docket No. SSA-2011-0019] RIN 0960-AH33 Technical Correction for Neurological Listing Cross-Reference AGENCY: Social Security Administration. ACTION: Final...

  14. Improving Vocational Education in Post-High School Institutions. Final Report.

    ERIC Educational Resources Information Center

    MacArthur, Earl W.

    Four ongoing programs in postsecondary vocational-technical education were examined in a national institute attended by 59 representatives from 31 states. Institutions reporting programs were: (1) Los Angeles Trade and Technical College, California, (2) Washtenaw Community College, Ann Arbor, Michigan, (3) Rockingham Community College, Wentworth,…

  15. Chicago Manufacturing Tech Prep. Fiscal Year 1991 Final Report.

    ERIC Educational Resources Information Center

    Chicago City Colleges, IL.

    During its first year of development in 1991, the Chicago Manufacturing Technical Preparation (Tech Prep) Program established a plan for implementing an industry-driven, articulated 4-year manufacturing technology course of study that integrates applied academic courses with technical courses and meets industry hiring standards. The project…

  16. 78 FR 29239 - Final Priority; Technical Assistance To Improve State Data Capacity-National Technical Assistance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-20

    ... reporting requirements. Changes: None. Focus TA on Assessment and Discipline Data Comment: Three commenters agreed with the importance of focusing on assessment and discipline data, and two commenters agreed with... decisions and actions associated with data collection and reporting. One commenter stated that assessment...

  17. Modular Radioisotope Thermoelectric Generator (RTG) Program. Final technical report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1992-12-31

    Section 2.0 of this report summarizes the MOD-RTG reference flight design, and Section 3.0 discusses the Ground Demonstration System design. Multicouple technology development is discussed in Section 4.0, and Section 5.0 lists all published technical papers prepared during the course of the contract.

  18. Methodology in Seeking Stakeholder Perceptions of Effective Technical Oral Presentations: An Exploratory Pilot Study

    ERIC Educational Resources Information Center

    Bhattacharyya, Ena; Patil, Arun; Sargunan, Rajeswary Appacutty

    2010-01-01

    Engineering communication studies indicate the importance of oral presentations as an indispensable component of workplace oral communication activities; however, since there is limited literature regarding stakeholder perceptions of effective presentation skills and attributes in technical oral presentations or final year engineering project…

  19. Additional Final Area Designations and Technical Amendment for the 2012 Annual Fine Particle Standard Established in 2012 - Mar 2015

    EPA Pesticide Factsheets

    EPA is establishing or revising initial area designations and a technical amendment to correct an inadvertent error in the initial designation for one area for the 2012 annual national ambient air quality standards for fine particle pollution.

  20. 75 FR 41404 - List of Approved Spent Fuel Storage Casks: NUHOMS®

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-16

    .... The NRC is taking this action because the applicant identified that a certain Technical Specification (TS) for Boral characterization was not written precisely. Specifically, the requirements for meeting... changes to the technical specifications. The NRC also published a direct final rule on May 6, 2010 (75 FR...

  1. Research in Automatic Russian-English Scientific and Technical Lexicography. Final Report.

    ERIC Educational Resources Information Center

    Wayne State Univ., Detroit, MI.

    Techniques of reversing English-Russian scientific and technical dictionaries into Russian-English versions through semi-automated compilation are described. Sections on manual and automatic processing discuss pre- and post-editing, the task program, updater (correction of errors and revision by specialist in a given field), the system employed…

  2. National Data Program for the Marine Environment Technical Development Plan. Final Report, Volume Two.

    ERIC Educational Resources Information Center

    System Development Corp., Santa Monica, CA.

    A national data program for the marine environment is recommended. Volume 2 includes: (1) objectives, scope, and methodology; (2) summary of the technical development plan; (3) agency development plans - Great Lakes and coastal development and (4) marine data network development plans. (Author)

  3. Course of Study Project. Final Report. Vocational-Technical Education Departmental Report, Vol. 17, No. 18.

    ERIC Educational Resources Information Center

    Pennsylvania State Univ., University Park. Div. of Occupational and Vocational Studies.

    Designed for use by area vocational-technical schools and other vocational programs, this project developed courses of study in eight occupational areas: commercial art, appliance repair, automotive mechanics, graphic arts, building trades maintenance, building construction trades, diesel mechanics, and welding. Course-of-study development…

  4. Seminar for Preparation of Professional Personnel for Vocational-Technical Education. Final Report.

    ERIC Educational Resources Information Center

    Dillon, Roy D.; Horner, James T.

    Seminar participants included college administrative officers, state vocational education directors, vocational-technical teacher educators, and Office of Education staff. The purpose of the June, 1968 seminar was to consider strategies for resolving critical vocational education personnel supply and demand problems. Presentations included in the…

  5. 75 FR 33167 - Technical Amendment Language Change From “Wholly” to “Fully”

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-11

    ... SOCIAL SECURITY ADMINISTRATION 20 CFR Parts 404, 405, 408, 416, and 418 [Docket No. SSA-2009-0062] RIN 0960-AH16 Technical Amendment Language Change From ``Wholly'' to ``Fully'' AGENCY: Social Security... these final rules, call Brian J. Rudick, Office of Regulations, Social Security Administration, 6401...

  6. 76 FR 58144 - Defense Federal Acquisition Regulation Supplement; Presumption of Development Exclusively at...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-20

    ... technical data package, in cases where the Government may have funded only a small portion of the... subcontractor's asserted restrictions on technical data and computer software. DATES: Effective date: September... data and computer software. More specifically, the final rule affects these validation procedures in...

  7. 75 FR 49408 - Navigation and Navigable Waters; Technical, Organizational, and Conforming Amendments, Bridges

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-13

    ... Conforming Amendments, Bridges AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: This rule makes non... technical corrections to Coast Guard bridge and navigable waters regulations. This rule will have no... announces or gathers public opinion or other information regarding bridge matters, nor will it change the...

  8. Research in network management techniques for tactical data communications networks

    NASA Astrophysics Data System (ADS)

    Boorstyn, R.; Kershenbaum, A.; Maglaris, B.; Sarachik, P.

    1982-09-01

    This is the final technical report for work performed on network management techniques for tactical data networks. It includes all technical papers that have been published during the control period. Research areas include Packet Network modelling, adaptive network routing, network design algorithms, network design techniques, and local area networks.

  9. A System for Evaluating Microcomputer Courseware for Vocational and Technical Education. Final Report.

    ERIC Educational Resources Information Center

    Chase, Shirley A.; And Others

    A project was conducted to design a system for evaluating microcomputer courseware for vocational and technical education. Through a literature review and contacts with organizations and individuals involved in courseware evaluation and use, project staff identified and acquired for review documents pertaining to courseware evaulation, vocational…

  10. Technical Report: Benchmarking for Quasispecies Abundance Inference with Confidence Intervals from Metagenomic Sequence Data

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McLoughlin, K.

    2016-01-22

    The software application “MetaQuant” was developed by our group at Lawrence Livermore National Laboratory (LLNL). It is designed to profile microbial populations in a sample using data from whole-genome shotgun (WGS) metagenomic DNA sequencing. Several other metagenomic profiling applications have been described in the literature. We ran a series of benchmark tests to compare the performance of MetaQuant against that of a few existing profiling tools, using real and simulated sequence datasets. This report describes our benchmarking procedure and results.

  11. Final Technical Report, Wind Generator Project (Ann Arbor)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Geisler, Nathan

    A Final Technical Report (57 pages) describing educational exhibits and devices focused on wind energy, and related outreach activities and programs. Project partnership includes the City of Ann Arbor, MI and the Ann Arbor Hands-on Museum, along with additional sub-recipients, and U.S. Department of Energy/Office of Energy Efficiency and Renewable Energy (EERE). Report relays key milestones and sub-tasks as well as numerous graphics and images of five (5) transportable wind energy demonstration devices and five (5) wind energy exhibits designed and constructed between 2014 and 2016 for transport and use by the Ann Arbor Hands-on Museum.

  12. Final Technical Report- Virginia Solar Pathways Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bond, Katharine; Cosby, Sarah

    This Report provides a technical review of the final results of a funding award to Virginia Electric and Power Company (Dominion Energy Virginia (DEV) or the Company) for a project under the U.S. Department of Energy’s Solar Energy Technologies Office. The three-year project was formally known as the Virginia Solar Pathways Project (VSPP or the Project). The purpose of the VSPP was to develop a collaborative utility-administered solar strategy (Solar Strategy) for DEV’s service territory in the Commonwealth that could serve as a replicable model for other states with similar policy environments.

  13. On the joint spectral density of bivariate random sequences. Thesis Technical Report No. 21

    NASA Technical Reports Server (NTRS)

    Aalfs, David D.

    1995-01-01

    For univariate random sequences, the power spectral density acts like a probability density function of the frequencies present in the sequence. This dissertation extends that concept to bivariate random sequences. For this purpose, a function called the joint spectral density is defined that represents a joint probability weighing of the frequency content of pairs of random sequences. Given a pair of random sequences, the joint spectral density is not uniquely determined in the absence of any constraints. Two approaches to constraining the sequences are suggested: (1) assume the sequences are the margins of some stationary random field, (2) assume the sequences conform to a particular model that is linked to the joint spectral density. For both approaches, the properties of the resulting sequences are investigated in some detail, and simulation is used to corroborate theoretical results. It is concluded that under either of these two constraints, the joint spectral density can be computed from the non-stationary cross-correlation.

  14. Loeffler 4.0: Diagnostic Metagenomics.

    PubMed

    Höper, Dirk; Wylezich, Claudia; Beer, Martin

    2017-01-01

    A new world of possibilities for "virus discovery" was opened up with high-throughput sequencing becoming available in the last decade. While scientifically metagenomic analysis was established before the start of the era of high-throughput sequencing, the availability of the first second-generation sequencers was the kick-off for diagnosticians to use sequencing for the detection of novel pathogens. Today, diagnostic metagenomics is becoming the standard procedure for the detection and genetic characterization of new viruses or novel virus variants. Here, we provide an overview about technical considerations of high-throughput sequencing-based diagnostic metagenomics together with selected examples of "virus discovery" for animal diseases or zoonoses and metagenomics for food safety or basic veterinary research. © 2017 Elsevier Inc. All rights reserved.

  15. M-X Environmental Technical Report. Socioeconomic Impact Estimates for White Pine County, Nevada. Detailed Tables.

    DTIC Science & Technology

    1980-12-22

    8217AP0A095 772 HENNINGSON DURHAM AND RICHARDSON SANTA BARBARA CA F/G 16/1 B-B ENVIRONMENTAL TECHNICAL REPORT. SOCIOECONOMIC IMPACT ESTIMA--ETC(U) DEC... Environmental Technical Reports,. 53ocioeconctnic’ Final r1eport Impact . Estimates for White Pine County, Nevada. ____________ Detailed Tables 6. PERFORMING...background information for the analysis contained in the M-X Deploment Area Selection and Land Withirawal/Acquisition Draft Environmental Impact Statement

  16. Optical fiber sensors for materials and structures characterization

    NASA Technical Reports Server (NTRS)

    Lindner, D. K.; Claus, R. O.

    1991-01-01

    The final technical report on Optical Fiber Sensors for Materials and Structures Characterization, covering the period August 1990 through August 1991 is presented. Research programs in the following technical areas are described; sapphire optical fiber sensors; vibration analysis using two-mode elliptical core fibers and sensors; extrinsic Fabry-Perot interferometer development; and coatings for fluorescent-based sensor. Research progress in each of these areas was substantial, as evidenced by the technical publications which are included as appendices.

  17. A joint university-government technical editing program

    NASA Technical Reports Server (NTRS)

    Stohrer, Freda F.; Pinelli, Thomas E.

    1978-01-01

    The NASA Langley Research Center (LaRC) and Old Dominion University have designed a mutually useful technical editing program. A university team made up of an English instructor and two graduate students - one from English, one from engineering - works with a senior editor from LaRC to prepare technical reports for publication. A round-robin technique gives the university team editorial commentary from both language and technical specialists; the senior editor from LaRC supervises reports through final publication. To date, the system has provided LaRC with a respectable product and university students with valuable on-the-job training.

  18. Report-reading patterns of technical managers and nonmanagers

    NASA Technical Reports Server (NTRS)

    Pinelli, T. E.; Cordle, V. M.; Glassman, M.; Vondran, R. F., Jr.

    1984-01-01

    A survey to determine the review and reading processes used by technical managers and nonmanagers indicates that the summary, abstract, conclusion, title page, and introduction are the components used most frequently by both groups to decide whether to read a NASA technical report. In the review process, significantly more managers than nonmanagers use the summary and conclusion, whereas significantly more nonmanagers use the abstract and title page. The most common sequence of review consists of the title page, abstract, and summary, in that order, for both groups. In the reading process, the conclusion, results and discussion, and summary are the components read by the highest percentage of both groups.

  19. Reduce costs with multimission sequencing and a multimission operations system

    NASA Technical Reports Server (NTRS)

    Bliss, D. A.; Morales, L. C.

    2003-01-01

    The paper will then propose extending this multi-mission philosophy to skeleton timeline development, science sequencing, and spacecraft sequencing. Finally, the paper will investigate a multi-mission approach to MOS development.

  20. Eco-innovative design approach: Integrating quality and environmental aspects in prioritizing and solving engineering problems

    NASA Astrophysics Data System (ADS)

    Chakroun, Mahmoud; Gogu, Grigore; Pacaud, Thomas; Thirion, François

    2014-09-01

    This study proposes an eco-innovative design process taking into consideration quality and environmental aspects in prioritizing and solving technical engineering problems. This approach provides a synergy between the Life Cycle Assessment (LCA), the nonquality matrix, the Theory of Inventive Problem Solving (TRIZ), morphological analysis and the Analytical Hierarchy Process (AHP). In the sequence of these tools, LCA assesses the environmental impacts generated by the system. Then, for a better consideration of environmental aspects, a new tool is developed, the non-quality matrix, which defines the problem to be solved first from an environmental point of view. The TRIZ method allows the generation of new concepts and contradiction resolution. Then, the morphological analysis offers the possibility of extending the search space of solutions in a design problem in a systematic way. Finally, the AHP identifies the promising solution(s) by providing a clear logic for the choice made. Their usefulness has been demonstrated through their application to a case study involving a centrifugal spreader with spinning discs.

  1. Current strategies for the restoration of adequate lordosis during lumbar fusion

    PubMed Central

    Barrey, Cédric; Darnis, Alice

    2015-01-01

    Not restoring the adequate lumbar lordosis during lumbar fusion surgery may result in mechanical low back pain, sagittal unbalance and adjacent segment degeneration. The objective of this work is to describe the current strategies and concepts for restoration of adequate lordosis during fusion surgery. Theoretical lordosis can be evaluated from the measurement of the pelvic incidence and from the analysis of spatial organization of the lumbar spine with 2/3 of the lordosis given by the L4-S1 segment and 85% by the L3-S1 segment. Technical aspects involve patient positioning on the operating table, release maneuvers, type of instrumentation used (rod, screw-rod connection, interbody cages), surgical sequence and the overall surgical strategy. Spinal osteotomies may be required in case of fixed kyphotic spine. AP combined surgery is particularly efficient in restoring lordosis at L5-S1 level and should be recommended. Finally, not one but several strategies may be used to achieve the need for restoration of adequate lordosis during fusion surgery. PMID:25621216

  2. Efficient experimental design and analysis strategies for the detection of differential expression using RNA-Sequencing

    PubMed Central

    2012-01-01

    Background RNA sequencing (RNA-Seq) has emerged as a powerful approach for the detection of differential gene expression with both high-throughput and high resolution capabilities possible depending upon the experimental design chosen. Multiplex experimental designs are now readily available, these can be utilised to increase the numbers of samples or replicates profiled at the cost of decreased sequencing depth generated per sample. These strategies impact on the power of the approach to accurately identify differential expression. This study presents a detailed analysis of the power to detect differential expression in a range of scenarios including simulated null and differential expression distributions with varying numbers of biological or technical replicates, sequencing depths and analysis methods. Results Differential and non-differential expression datasets were simulated using a combination of negative binomial and exponential distributions derived from real RNA-Seq data. These datasets were used to evaluate the performance of three commonly used differential expression analysis algorithms and to quantify the changes in power with respect to true and false positive rates when simulating variations in sequencing depth, biological replication and multiplex experimental design choices. Conclusions This work quantitatively explores comparisons between contemporary analysis tools and experimental design choices for the detection of differential expression using RNA-Seq. We found that the DESeq algorithm performs more conservatively than edgeR and NBPSeq. With regard to testing of various experimental designs, this work strongly suggests that greater power is gained through the use of biological replicates relative to library (technical) replicates and sequencing depth. Strikingly, sequencing depth could be reduced as low as 15% without substantial impacts on false positive or true positive rates. PMID:22985019

  3. Efficient experimental design and analysis strategies for the detection of differential expression using RNA-Sequencing.

    PubMed

    Robles, José A; Qureshi, Sumaira E; Stephen, Stuart J; Wilson, Susan R; Burden, Conrad J; Taylor, Jennifer M

    2012-09-17

    RNA sequencing (RNA-Seq) has emerged as a powerful approach for the detection of differential gene expression with both high-throughput and high resolution capabilities possible depending upon the experimental design chosen. Multiplex experimental designs are now readily available, these can be utilised to increase the numbers of samples or replicates profiled at the cost of decreased sequencing depth generated per sample. These strategies impact on the power of the approach to accurately identify differential expression. This study presents a detailed analysis of the power to detect differential expression in a range of scenarios including simulated null and differential expression distributions with varying numbers of biological or technical replicates, sequencing depths and analysis methods. Differential and non-differential expression datasets were simulated using a combination of negative binomial and exponential distributions derived from real RNA-Seq data. These datasets were used to evaluate the performance of three commonly used differential expression analysis algorithms and to quantify the changes in power with respect to true and false positive rates when simulating variations in sequencing depth, biological replication and multiplex experimental design choices. This work quantitatively explores comparisons between contemporary analysis tools and experimental design choices for the detection of differential expression using RNA-Seq. We found that the DESeq algorithm performs more conservatively than edgeR and NBPSeq. With regard to testing of various experimental designs, this work strongly suggests that greater power is gained through the use of biological replicates relative to library (technical) replicates and sequencing depth. Strikingly, sequencing depth could be reduced as low as 15% without substantial impacts on false positive or true positive rates.

  4. Mature Programs of Study: Examining Policy Implementation at the Local Level. Final Report

    ERIC Educational Resources Information Center

    Alfeld, Corinne; Bhattacharya, Sharika

    2013-01-01

    The 2006 Carl D. Perkins Career and Technical Education Act required that all career technical education (CTE) programs offer secondary to postsecondary programs of study (POS), which integrate rigorous academics, offer dual enrollment options, and lead to an industry-recognized degree or credential. Focused on improving students' transition to…

  5. 75 FR 37722 - OMB Approvals Under the Paperwork Reduction Act; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-30

    ... since its last update. B. Why is this Technical Amendment Issued as a Final Rule? The information... provisions since the last update of this table. The paperwork burden associated with these new provisions was... amendment updates the table that lists the Office of Management and Budget (OMB) control numbers issued...

  6. United States Air Force Training Line Simulator. Final Report.

    ERIC Educational Resources Information Center

    Nauta, Franz; Pierce, Michael B.

    This report describes the technical aspects and potential applications of a computer-based model simulating the flow of airmen through basic training and entry-level technical training. The objective of the simulation is to assess the impacts of alternative recruit classification and training policies under a wide variety of assumptions regarding…

  7. Final recovery plan of the southwestern willow flycatcher (Empidonax traillii extimus)

    Treesearch

    Deborah M. Finch; Stephen I. Rothstein; Jon C. Boren; William L. Graf; Jerry L. Holechek; Barbara E. Kus; Robert M. Marshall; Molly M. Pohl; Susan J. Sferra; Mark K. Sogge; Julie C. Stromberg; Bradley A. Valentine; Mary J. Whitfield; Sartor O. Williams

    2002-01-01

    The Southwestern Willow Flycatcher Recovery Team is composed of a Technical Subgroup (pg. ii), six Implementation Subgroups (Appendix A), and a Tribal Working Group. The Technical Subgroup consists of 14 academic scientists, researchers, and resource managers with a wide range of expertise in avian biology and ecology, southwestern willow flycatcher ecology, cowbird...

  8. Local/State Bilingual Project. 1981-82 Final Technical Report. Appendixes.

    ERIC Educational Resources Information Center

    Austin Independent School District, TX. Office of Research and Evaluation.

    The 1981-82 Local/State Bilingual Program Technical Report addresses the evaluation questions of the Local/State Bilingual Program Evaluation Design. It is organized into six appendixes. Each appendix reports the information collected by a specific measure. Each appendix consists of (1) an instrument description, (2) purpose of the measure, (3)…

  9. 77 FR 26444 - Revisions to Final Response To Petition From New Jersey Regarding SO2

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-04

    ... modeling or other technical analyses and no new analyses were necessary to make the revisions. III. Public... this modeling approach. Therefore, no new technical analyses or any changes to the modeling are...) modeling analysis submitted with the September 2010 petition identified NAAQS violations at receptors in...

  10. 77 FR 45282 - NRC Position on the Relationship Between General Design Criteria and Technical Specification...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-31

    ..., are described in the final safety analysis report (FSAR). The staff safety evaluation documents the acceptability of these analyses, and it is the combination of the FSAR analyses and the staff safety evaluation... analysis, maintain their capability to perform their safety functions. Technical Specification Operability...

  11. A Dissemination Model for New Technical Education Programs. Final Report.

    ERIC Educational Resources Information Center

    Hull, Daniel M.

    The Technical Education Research Center-SW has conceived, tested, and refined a model for disseminating newly developed programs and materials throughout the nation. The model performed successfully in the dissemination of more than 50,000 educational units (modules) of Laser/Electro-Optics Technician (LEOT) materials during a four-year period…

  12. 75 FR 28499 - Medical Certification Requirements as Part of the Commercial Driver's License (CDL); Technical...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-21

    ... Driver's License (CDL); Technical, Organizational, and Conforming Amendments AGENCY: Federal Motor... ``medical examiner's license or certificate number'' to refer to the number on a medical examiner's license... examiner qualifies him or her to drive. This inconsistency has been clarified in today's final rule so that...

  13. Installation and Assimilation of Educational Innovations in Vocational/Technical Programs in Post-Secondary Institutions. Final Report.

    ERIC Educational Resources Information Center

    Appel, Victor H.; Roueche, John E.

    The factors which contribute to the successful installation and assimilation of educational innovations were examined in relation to vocational/technical programs in eight post-secondary institutions. About 555 instructors, administrators, and non-teaching professional staff were surveyed regarding the level and extent of use of individualized…

  14. Final Technical Report on the Institute for Advanced Study in Student Personnel Work.

    ERIC Educational Resources Information Center

    Callis, Robert

    This document reports the planning, implementation, and evaluation of a 9-month institute held at the University of Missouri-Columbia to prepare participants (approximately 20) for leadership positions in student personnel work at junior colleges and technical institutes. The following aspects of the instructional program are discussed and…

  15. Demonstration and Research Program for Teaching Young String Players. Final Report.

    ERIC Educational Resources Information Center

    Yarborough, William

    This report explains a system for rapidly training beginning students in the technical aspects of playing a stringed instrument. The program also affords them a well-rounded, basic knowledge of music. A "numerical" method of notation and concentrated muscular exercises greatly speeded the technical learning process. The daily coordination of ear…

  16. Troubleshooting Instruction in Vocational-Technical Education Via Dynamic Simulation. Final Report.

    ERIC Educational Resources Information Center

    Finch, Curtis R.

    This study was designed to examine the feasibility of using simulation as a means of teaching vocational-technical students to detect and identify malfunctions in selected electrical and mechanical systems. A dynamic simulator was employed which features interchangeable panels and logic that permits the simulation of electrical or mechanical…

  17. Special Project for Research Training in Vocational Education: Research Training Series. Final Report.

    ERIC Educational Resources Information Center

    Vivian, Neal E.

    To upgrade research and research utilization competence of vocational educators, The Center for Vocational and Technical Education and The American Vocational Association planned four 1-week research training programs on: (1) Planning Vocational/Technical Education Programs Based on Manpower Research, (2) Patterns of Career Development as Applied…

  18. 78 FR 4030 - Designation of Low-Income Status; Acceptance of Secondary Capital Accounts by Low-Income...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-18

    ... writing that it wishes to accept the designation. The final rule extends an FCU's response time from 30..., nonsubstantive technical amendments to NCUA's requirements for insurance regulation to reflect current agency... requirements for insurance regulation. These technical corrections are necessary to reflect current agency...

  19. SURVEY OF INFORMATION ON VOCATIONAL AND TECHNICAL EDUCATION IN THE STATE OF ILLINOIS. FINAL REPORT.

    ERIC Educational Resources Information Center

    Corplan Associates, Chicago, IL. Technology Center.

    THE BASIC OBJECTIVE OF THE SURVEY WAS TO GATHER INFORMATION HELPFUL IN PLANNING AND DEVELOPING VOCATIONAL AND TECHNICAL EDUCATION PRIMARILY WITHIN THE PUBLIC SCHOOL SYSTEM. OCCUPATIONAL NEEDS WERE IDENTIFIED FROM FORECASTS OF CHANGES IN CURRENT OCCUPATIONS, AN ANALYSIS OF THE IMPLICATIONS OF SCIENTIFIC AND TECHNOLOGICAL DEVELOPMENTS, AND…

  20. 75 FR 47211 - Navigation and Navigable Waters; Technical, Organizational, and Conforming Amendments, Sector...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-05

    ... Puget Sound, WA AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: This rule makes non-substantive... technical corrections to reflect the renaming of Sector Seattle to Sector Puget Sound as part of the Coast... organizational structure. Sector Seattle has been disestablished and Sector Puget Sound has been established in...

  1. Statewide Curriculum Articulation Project for Vocational-Technical Education in Minnesota. Final Report.

    ERIC Educational Resources Information Center

    Burger, Laura J.; And Others

    The goal of this project was to develop, validate, and utilize a process for vertically articulating curriculum between secondary and post secondary levels of vocational technical education throughout the state of Minnesota. Procedures involved the identification of two areas of staff responsibility: research and development, and service to local…

  2. Delivery of Instruction via Television. Final Report.

    ERIC Educational Resources Information Center

    Wisconsin State Board of Vocational, Technical, and Adult Education, Madison.

    During 1985, the Wisconsin Board of Vocational, Technical, and Adult Education (VTAE) expanded its delivery of instruction via television. Approximately 3,000 persons at 170 sites throughout the United States and Canada participated in the VTAE's Technical School of the Air. In addition to delivering six to eight courses per semester, the network…

  3. O*NET Final Technical Report. Volume I [and] Volume II [and] Volume III.

    ERIC Educational Resources Information Center

    Peterson, Norman G.; Mumford, Michael D.; Borman, Walter C.; Jeanneret, P. Richard; Fleishman, Edwin A.; Levin, Kerry Y.

    This document contains the three volumes of the technical report for development of the prototype of the Occupational Information Network (O*NET), which is intended to replace the "Dictionary of Occupational Titles.""General Introduction" (Norman G. Peterson) presents an overview of O*NET's purpose, content, and structure.…

  4. 78 FR 34920 - Technical Amendments To Reflect the Authorizing Legislation of the Institute of Museum and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-11

    ... NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES Institute of Museum and Library Services 45 CFR... Museum and Library Services AGENCY: Institute of Museum and Library Services (IMLS), NFAH. ACTION: Technical amendment; final rule. SUMMARY: The Institute of Museum and Library amends its grants regulations...

  5. Final report on technical work accomplished under contract NASw-2953

    NASA Technical Reports Server (NTRS)

    Fredricks, R. W.

    1977-01-01

    A report is given on the technical work accomplished in the area of plasma physics. The subjects covered are: (1) oblique whistler instabilities, (2) current-limited electron beam injection, (3) three-dimensional ion sound turbulence, (4) theoretical aspects of sounder antenna operation and (5) whistler modes in bow shock structures.

  6. Development of German-English Machine Translation System. Final Technical Report.

    ERIC Educational Resources Information Center

    Lehmann, Winfred P.; Stachowitz, Rolf A.

    This report describes work on a pilot system for a fully automatic, high-quality translation of German scientific and technical text into English and gives the results of an experiment designed to show the system's capability to produce quality mechanical translation. The areas considered were: (1) grammar formalism, mainly involving the addition…

  7. An Optimized Transient Dual Luciferase Assay for Quantifying MicroRNA Directed Repression of Targeted Sequences

    PubMed Central

    Moyle, Richard L.; Carvalhais, Lilia C.; Pretorius, Lara-Simone; Nowak, Ekaterina; Subramaniam, Gayathery; Dalton-Morgan, Jessica; Schenk, Peer M.

    2017-01-01

    Studies investigating the action of small RNAs on computationally predicted target genes require some form of experimental validation. Classical molecular methods of validating microRNA action on target genes are laborious, while approaches that tag predicted target sequences to qualitative reporter genes encounter technical limitations. The aim of this study was to address the challenge of experimentally validating large numbers of computationally predicted microRNA-target transcript interactions using an optimized, quantitative, cost-effective, and scalable approach. The presented method combines transient expression via agroinfiltration of Nicotiana benthamiana leaves with a quantitative dual luciferase reporter system, where firefly luciferase is used to report the microRNA-target sequence interaction and Renilla luciferase is used as an internal standard to normalize expression between replicates. We report the appropriate concentration of N. benthamiana leaf extracts and dilution factor to apply in order to avoid inhibition of firefly LUC activity. Furthermore, the optimal ratio of microRNA precursor expression construct to reporter construct and duration of the incubation period post-agroinfiltration were determined. The optimized dual luciferase assay provides an efficient, repeatable and scalable method to validate and quantify microRNA action on predicted target sequences. The optimized assay was used to validate five predicted targets of rice microRNA miR529b, with as few as six technical replicates. The assay can be extended to assess other small RNA-target sequence interactions, including assessing the functionality of an artificial miRNA or an RNAi construct on a targeted sequence. PMID:28979287

  8. The Independent Technical Analysis Process Final Report 2006-2007.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Duberstein, Corey; Ham, Kenneth; Dauble, Dennis

    2007-03-01

    The Bonneville Power Administration (BPA) contracted with the Pacific Northwest National Laboratory (PNNL) to provide technical analytical support for system-wide fish passage information (BPA Project No. 2006-010-00). The goal of this project was to produce rigorous technical analysis products using independent analysts and anonymous peer reviewers. This project provided an independent technical source for non-routine fish passage analyses while allowing routine support functions to be performed by other well-qualified entities. The Independent Technical Analysis Process (ITAP) was created to provide non-routine analysis for fish and wildlife agencies and tribes in particular and the public in general on matters related tomore » juvenile and adult salmon and steelhead passage through the mainstem hydrosystem. The process was designed to maintain the independence of analysts and reviewers from parties requesting analyses, to avoid potential bias in technical products. The objectives identified for this project were to administer a rigorous, transparent process to deliver unbiased technical assistance necessary to coordinate recommendations for storage reservoir and river operations that avoid potential conflicts between anadromous and resident fish. Seven work elements, designated by numbered categories in the Pisces project tracking system, were created to define and accomplish project goals as follows: (1) 118 Coordination - Coordinate technical analysis and review process: (a) Retain expertise for analyst/reviewer roles. (b) Draft research directives. (c) Send directive to the analyst. (d) Coordinate two independent reviews of the draft report. (e) Ensure reviewer comments are addressed within the final report. (2) 162 Analyze/Interpret Data - Implement the independent aspects of the project. (3) 122 Provide Technical Review - Implement the review process for the analysts. (4) 132 Produce Annual Report - FY06 annual progress report with Pisces Disseminate (5) 161 Disseminate Raw/Summary Data and Results - Post technical products on the ITAP web site. (6) 185-Produce Pisces Status Report - Provide periodic status reports to BPA. (7) 119 Manage and Administer Projects - project/contract administration.« less

  9. Accounting for technical noise in differential expression analysis of single-cell RNA sequencing data.

    PubMed

    Jia, Cheng; Hu, Yu; Kelly, Derek; Kim, Junhyong; Li, Mingyao; Zhang, Nancy R

    2017-11-02

    Recent technological breakthroughs have made it possible to measure RNA expression at the single-cell level, thus paving the way for exploring expression heterogeneity among individual cells. Current single-cell RNA sequencing (scRNA-seq) protocols are complex and introduce technical biases that vary across cells, which can bias downstream analysis without proper adjustment. To account for cell-to-cell technical differences, we propose a statistical framework, TASC (Toolkit for Analysis of Single Cell RNA-seq), an empirical Bayes approach to reliably model the cell-specific dropout rates and amplification bias by use of external RNA spike-ins. TASC incorporates the technical parameters, which reflect cell-to-cell batch effects, into a hierarchical mixture model to estimate the biological variance of a gene and detect differentially expressed genes. More importantly, TASC is able to adjust for covariates to further eliminate confounding that may originate from cell size and cell cycle differences. In simulation and real scRNA-seq data, TASC achieves accurate Type I error control and displays competitive sensitivity and improved robustness to batch effects in differential expression analysis, compared to existing methods. TASC is programmed to be computationally efficient, taking advantage of multi-threaded parallelization. We believe that TASC will provide a robust platform for researchers to leverage the power of scRNA-seq. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  10. Accounting for technical noise in differential expression analysis of single-cell RNA sequencing data

    PubMed Central

    Jia, Cheng; Hu, Yu; Kelly, Derek; Kim, Junhyong

    2017-01-01

    Abstract Recent technological breakthroughs have made it possible to measure RNA expression at the single-cell level, thus paving the way for exploring expression heterogeneity among individual cells. Current single-cell RNA sequencing (scRNA-seq) protocols are complex and introduce technical biases that vary across cells, which can bias downstream analysis without proper adjustment. To account for cell-to-cell technical differences, we propose a statistical framework, TASC (Toolkit for Analysis of Single Cell RNA-seq), an empirical Bayes approach to reliably model the cell-specific dropout rates and amplification bias by use of external RNA spike-ins. TASC incorporates the technical parameters, which reflect cell-to-cell batch effects, into a hierarchical mixture model to estimate the biological variance of a gene and detect differentially expressed genes. More importantly, TASC is able to adjust for covariates to further eliminate confounding that may originate from cell size and cell cycle differences. In simulation and real scRNA-seq data, TASC achieves accurate Type I error control and displays competitive sensitivity and improved robustness to batch effects in differential expression analysis, compared to existing methods. TASC is programmed to be computationally efficient, taking advantage of multi-threaded parallelization. We believe that TASC will provide a robust platform for researchers to leverage the power of scRNA-seq. PMID:29036714

  11. Site Operator technical report. Final report (1992--1996)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1996-12-01

    The Southern California Edison Company (SCE) and the US Department of Energy (DOE) entered into cooperative agreement No. DE-FC07-91ID13077 on August 23, 1991, which expired on August 3, 1996. This cooperative agreement provided SCE with DOE cofunding for participation in the DOE`s Electric and Hybrid Vehicle Site Operator Program. In return, SCE provided the DOE with quarterly progress reports which include operating and maintenance data for the electric (EVs) vehicles in SCE`s fleet. Herein is SCE`s final report for the 1992 to 1996 agreement period. As of September 1, 1996 the SCE fleet had 65 electric vehicles in service. Amore » total of 578,200 miles had been logged. During the agreement period, SCE sent the DOE a total of 19 technical reports (Appendix B). This report summarizes the technical achievements which took place during a long, productive and rewarding, relationship with the DOE.« less

  12. 7 CFR 1944.419 - Final grantee evaluation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) PROGRAM REGULATIONS (CONTINUED) HOUSING Self-Help Technical Assistance Grants § 1944.419 Final grantee... Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS..., application, this regulation, and any amendments. (a) This is a quantitative evaluation of the grantee to...

  13. ECLSS medical support activities

    NASA Technical Reports Server (NTRS)

    Crump, William J.; Kilgore, Melvin V., Jr.

    1991-01-01

    During the period from April 10, 1990 to April 9, 1991, the Consortium for the Space Life Sciences provided technical assistance to the NASA/MSFC water recovery efforts. This assistance was in the form of literature reviews, technical recommendations, and presentations. This final report summarizes the activities completed during this period and identifies those areas requiring additional efforts. The tasks which the University of Alabama in Huntsville (UAH) water recovery team addressed were either identified by MSFC technical representatives or chosen from those outlined in the subject statement of work.

  14. Switching gears and changing lanes

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hammock, Earlene C.

    2002-01-01

    From musing over Shakespeare's fine lines and metaphors to teaching technical writing and editing, and finally, to cranking out scientific and technical documents, writing articles, and editing publications-how'd a nice girl like me end up in a place like this? Twice, after having prepared for an academic career of teaching and research, I found myself in a technical communications position-first, at the University of Texas at El Paso College of Engineering and later, at Los Alamos National Laboratory. What happened?

  15. NASA/DOD Aerospace Knowledge Diffusion Research Project. Paper 32: A new era in international technical communication: American-Russian collaboration

    NASA Technical Reports Server (NTRS)

    Flammia, Madelyn; Barclay, Rebecca O.; Pinelli, Thomas E.; Keene, Michael L.; Burger, Robert H.; Kennedy, John M.

    1993-01-01

    Until the recent dissolution of the Soviet Union, the Communist Party exerted a strict control of access to and dissemination of scientific and technical information (STI). This article presents models of the Soviet-style information society and the Western-style information society and discusses the effects of centralized governmental control of information on Russian technical communication practices. The effects of political control on technical communication are then used to interpret the results of a survey of Russian and U.S. aerospace engineers and scientists concerning the time devoted to technical communication, their collaborative writing practices and their attitudes toward collaboration, the kinds of technical documents they produce and use, their views regarding the appropriate content for an undergraduate technical communication course, and their use of computer technology. Finally, the implications of these findings for future collaboration between Russian and U.S. engineers and scientists are examined.

  16. NASA/DoD Aerospace Knowledge Diffusion Research Project. XXXII - A new era in international technical communication: American-Russian collaboration

    NASA Technical Reports Server (NTRS)

    Flammia, Madelyn; Barclay, Rebecca O.; Pinelli, Thomas E.; Keene, Michael L.; Burger, Robert H.; Kennedy, John M.

    1993-01-01

    Until the recent dissolution of the Soviet Union, the Communist Party exerted a strict control of access to and dissemination of scientific and technical information. This article presents models of the Soviet-style information society and the Western-style information society and discusses the effects of centralized governmental control of information on Russian technical communication practices. The effects of political control on technical communication are then used to interpret the results of a survey of Russian and U.S. aerospace engineers and scientists concerning the time devoted to technical communication, their collaborative writing practices and their attitudes toward collaboration, the kinds of technical documents they produce and use, their views regarding the appropriate content for an undergraduate technical communication course, and their use of computer technology. Finally, the implications of these findings for future collaboration between Russian and U.S. engineers and scientists are examined.

  17. Rewriting the Central European Early Bronze Age Chronology: Evidence from Large-Scale Radiocarbon Dating

    PubMed Central

    Knipper, Corina; Friedrich, Ronny; Kromer, Bernd; Lindauer, Susanne; Radosavljević, Jelena; Wittenborn, Fabian; Krause, Johannes

    2015-01-01

    The transition from the Neolithic to the Early Bronze Age in Central Europe has often been considered as a supra-regional uniform process, which led to the growing mastery of the new bronze technology. Since the 1920s, archaeologists have divided the Early Bronze Age into two chronological phases (Bronze A1 and A2), which were also seen as stages of technical progress. On the basis of the early radiocarbon dates from the cemetery of Singen, southern Germany, the beginning of the Early Bronze Age in Central Europe was originally dated around 2300/2200 BC and the transition to more complex casting techniques (i.e., Bronze A2) around 2000 BC. On the basis of 140 newly radiocarbon dated human remains from Final Neolithic, Early and Middle Bronze Age cemeteries south of Augsburg (Bavaria) and a re-dating of ten graves from the cemetery of Singen, we propose a significantly different dating range, which forces us to re-think the traditional relative and absolute chronologies as well as the narrative of technical development. We are now able to date the beginning of the Early Bronze Age to around 2150 BC and its end to around 1700 BC. Moreover, there is no transition between Bronze (Bz) A1 and Bronze (Bz) A2, but a complete overlap between the type objects of the two phases from 1900–1700 BC. We thus present a revised chronology of the assumed diagnostic type objects of the Early Bronze Age and recommend a radiocarbon-based view on the development of the material culture. Finally, we propose that the traditional phases Bz A1 and Bz A2 do not represent a chronological sequence, but regionally different social phenomena connected to the willingness of local actors to appropriate the new bronze technology. PMID:26488413

  18. A robust method to analyze copy number alterations of less than 100 kb in single cells using oligonucleotide array CGH.

    PubMed

    Möhlendick, Birte; Bartenhagen, Christoph; Behrens, Bianca; Honisch, Ellen; Raba, Katharina; Knoefel, Wolfram T; Stoecklein, Nikolas H

    2013-01-01

    Comprehensive genome wide analyses of single cells became increasingly important in cancer research, but remain to be a technically challenging task. Here, we provide a protocol for array comparative genomic hybridization (aCGH) of single cells. The protocol is based on an established adapter-linker PCR (WGAM) and allowed us to detect copy number alterations as small as 56 kb in single cells. In addition we report on factors influencing the success of single cell aCGH downstream of the amplification method, including the characteristics of the reference DNA, the labeling technique, the amount of input DNA, reamplification, the aCGH resolution, and data analysis. In comparison with two other commercially available non-linear single cell amplification methods, WGAM showed a very good performance in aCGH experiments. Finally, we demonstrate that cancer cells that were processed and identified by the CellSearch® System and that were subsequently isolated from the CellSearch® cartridge as single cells by fluorescence activated cell sorting (FACS) could be successfully analyzed using our WGAM-aCGH protocol. We believe that even in the era of next-generation sequencing, our single cell aCGH protocol will be a useful and (cost-) effective approach to study copy number alterations in single cells at resolution comparable to those reported currently for single cell digital karyotyping based on next generation sequencing data.

  19. Communication Platform Payload Definition (CPPD) study. Volume 2: Technical report

    NASA Technical Reports Server (NTRS)

    Hunter, E. M.; Driggers, T.; Jorasch, R.

    1986-01-01

    This is Volume 2 (Technical Report) of the Ford Aerospace & Communications Corporation Final Report for the Communication Platform Payload Definition (CPPD) Study program conducted for NASA Lewis Research Center under contract No. NAS3-24235. This report presents the results of the study effort leading to five potential platform payloads to service CONUS and WARC Region 2 traffic demand as projected to the year 2008. The report addresses establishing the data bases, developing service aggregation scenarios, selecting and developing 5 payload concepts, performing detailed definition of the 5 payloads, costing them, identifying critical technology, and finally comparing the payloads with each other and also with non-aggregated equivalent services.

  20. Science sequence design

    NASA Technical Reports Server (NTRS)

    Koskela, P. E.; Bollman, W. E.; Freeman, J. E.; Helton, M. R.; Reichert, R. J.; Travers, E. S.; Zawacki, S. J.

    1973-01-01

    The activities of the following members of the Navigation Team are recorded: the Science Sequence Design Group, responsible for preparing the final science sequence designs; the Advanced Sequence Planning Group, responsible for sequence planning; and the Science Recommendation Team (SRT) representatives, responsible for conducting the necessary sequence design interfaces with the teams during the mission. The interface task included science support in both advance planning and daily operations. Science sequences designed during the mission are also discussed.

  1. Best Practices for Continuous Monitoring of Temperature and Flow in Wadeable Streams (Final Report)

    EPA Science Inventory

    This final report is a technical "best practices" document describing sensor deployment for and collection of continuous temperature and flow data at ungaged sites in wadeable streams. This document addresses questions related to equipment needs; configuration, placement, and ins...

  2. Hazardous Waste Management System - Identification and Listing of Hazardous Waste - Recycled Used Oil Management Standards - Federal Register Notice, May 3, 1993

    EPA Pesticide Factsheets

    This action corrects several technical errors and provides clarifying amendments to the final recycled used oil management standards rule. The final rule was published on September 10, 1992 (57 FR 41566).

  3. 75 FR 6839 - Technical Service Provider Assistance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-12

    ... Executive Order 12866, the Office of Management and Budget determined that this final rule is not a... or are eligible to participate in conservation programs to help them make land management decisions...: Covered Programs. The interim final rule incorporated reference to the Agricultural Management Assistance...

  4. Charlotte Circle Outreach. Final Report.

    ERIC Educational Resources Information Center

    Calhoun, Mary Lynne; Rose, Terry L.; Prendergast, Donna

    This final report details the activities of the Charlotte Circle Outreach, a program designed to provide technical assistance and training to early intervention programs offering services to infants and young children with substantial disabilities, ages birth through two years. This mission was accomplished through cooperative planning with…

  5. Determination of the Biologically Relevant Sampling Depth for Terrestrial and Aquatic Ecological Risk Assessments (Final Report)

    EPA Science Inventory

    The Ecological Risk Assessment Support Center (ERASC) announced the release of the final report, Determination of the Biologically Relevant Sampling Depth for Terrestrial and Aquatic Ecological Risk Assessments. This technical paper provides defensible approximations fo...

  6. Production Scheduling of Sequenced Tapes for Printed Circuit Pack Assembly.

    DTIC Science & Technology

    1987-07-09

    detail. L j 6 The subject matter of this thesis is inspired directly from their technical report. The goals of this research are twofold: 1) Test their...The subject matter of the following chapters describes a heuristic approach to another variation of the sequenced tape production scheduling problem...assignment problem, comprise the subject matter of Chapter 5. It is sufficient to note that the three definitions of the term common correspond to the

  7. A Program of Technical Assistance to Industry in Twenty-Six Mississippi Counties and the Choctaw Indian Reservation. Final Report.

    ERIC Educational Resources Information Center

    Phillips, Jane; Sewell, Charles

    A broad technical assistance program has been established in 25 EDA (Economic Development Administration) contract counties and on the Choctaw Indian Reservation Nashoba County) to stimulate new job opportunities by solving operational problems which limit the expansion and diversification of existing industry; professional services in evaluating…

  8. For Work-Force Training, a Plan to Give College Credit Where It's Due

    ERIC Educational Resources Information Center

    Sander, Libby

    2008-01-01

    After nearly three years of planning, Ohio's higher-education officials are finalizing an ambitious program to grant college credit for some technical courses offered at the state's adult-education centers. The program, called the Career-Technical Credit Transfer, is the latest in a string of state efforts to more closely link work-force training…

  9. Improving the Dissemination of Scientific and Technical Information: A Practitioner's Guide to Innovation. (Final Report).

    ERIC Educational Resources Information Center

    Capital Systems Group, Inc., Rockville, MD.

    The aim of this guide is to alert persons with an operational interest in scientific communication to new ideas, techniques, and equipment in the field of communication media and publications. The focus is on the dissemination of scientific information via the technical journal or its equivalent. Secondary dissemination of information such as…

  10. Unified Technical Concepts--Phase II. Expand Application to Industrial Technologies and Adult Education. Final Report.

    ERIC Educational Resources Information Center

    Technical Education Research Center, Waco, TX.

    A project was conducted to develop a laboratory-based instructional system in physics for two-year technician programs that emphasizes both the analogies between basic physical principles and the applications of the principles in modern technology. The Unified Technical Concepts (UTC) system that was developed is (1) a reorganization of physics…

  11. THE DEVELOPMENT OF ACHIEVEMENT MEASURES FOR TRADE AND TECHNICAL EDUCATION. PROGRESS REPORT NUMBER THREE.

    ERIC Educational Resources Information Center

    BALDWIN, THOMAS S.

    DURING THIS PERIOD FROM SEPTEMBER 1 THROUGH NOVEMBER 30, 1966, 35 FIELD CONSULTANTS COMPLETED ANALYSIS OF THEIR INDIVIDUAL TRADE AND TECHNICAL CURRICULUMS. THESE ANALYSES WERE DEVELOPED INTO AN OUTLINE TO SERVE AS A GUIDE FOR DEVELOPING ACHIEVEMENT TESTS. THE FINAL OUTLINE WAS DIVIDED INTO AS MANY DIFFERENT AREAS AS THE CONSULTANTS FELT NECESSARY…

  12. Word Lists to Simplify Vocabulary of Technical Information. Final Report.

    ERIC Educational Resources Information Center

    Kincaid, J. Peter; And Others

    This report describes eight word lists developed for use as part of the computer readability editing system (CRES), which was developed to serve as an author's aid in improving the ease of comprehending Navy technical manuals and training materials. The system has features which flag uncommon and misspelled words and long sentences, suggest simple…

  13. 77 FR 1889 - Drivers of CMVs: Restricting the Use of Cellular Phones; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-12

    ... DEPARTMENT OF TRANSPORTATION Federal Motor Carrier Safety Administration 49 CFR Part 391 [Docket No. FMCSA-2010-0096] RIN 2126-AB29 Drivers of CMVs: Restricting the Use of Cellular Phones; Technical... Cellular Phones final rule (76 FR 75470) had a clerical error in Sec. 391.15(f)(1) that stated ``paragraph...

  14. 27 Years of Impact: Vocational-Technical Education in Ohio. Final Annual Report under Federal Law.

    ERIC Educational Resources Information Center

    Ohio State Council on Vocational Education, Westerville.

    The Ohio Council on Vocational Education (OCOVE) was created to strengthen the career, vocational, and technical education services provided for Ohioans as a practical, efficient, and sure way to enhance the competitiveness of individual workers and the state and national economy. Some of the accomplishments of OCOVE during its 27 years of…

  15. Vocational-Technical Education Interface with Ohio's High Technology Business and Industrial Sector. Final Report.

    ERIC Educational Resources Information Center

    McCormick, Robert W.

    This study explored the relationship of vocational-technical educational institutions in Ohio with business and industry using high-technology applications. The study attempted to determine what high-technology applications will be adopted by Ohio's business and industry in the next 5 years, what experience the schools have had in working with…

  16. National Institute of Statistical Sciences Data Confidentiality Technical Panel: Final Report. NCES 2011-608

    ERIC Educational Resources Information Center

    Karr, Alan

    2011-01-01

    NCES asked the National Institute of Statistical Sciences (NISS) to convene a technical panel of survey and policy experts to examine the NCES current and planned data dissemination strategies for confidential data with respect to: mandates and directives that NCES make data available; current and prospective technologies for protecting and…

  17. [Municipalities as a Model for New Careers and Redirection of Vocational-Technical Education Programs.] Final Report.

    ERIC Educational Resources Information Center

    Institute for Local Self Government, Berkeley, CA.

    To meet the manpower needs of local governments, the model developed for this project redirects national and technical education toward new careers programs. Designed by task forces of professional personnel, the model utilizes existing local government resources, including funds for new career activities. Accomplishments of the project include:…

  18. Social Science Research Institutes in the Quality American University. Final Technical Report.

    ERIC Educational Resources Information Center

    Totman, Theodore L.

    The technical report presents a chapter outline and thesis summary of an investigation of social science research institutes in American universities. The bulk of the report presents the thesis in four sections. Section I proposes a typology of organized social research units (OSRUs) in the 11 universities studied. Dimensions used to classify the…

  19. Expanding Horizons: A Program for Students Entering Nontraditional Technical Occupations. PY95 Final Detailed Report.

    ERIC Educational Resources Information Center

    Austin Community Coll., TX.

    The Expanding Horizons Project at Austin Community College successfully achieved its goals for Project Year 1994-95. During the year, the project accomplished the following: raised public awareness of the need to overcome gender bias, promoted career opportunities in nontraditional technical occupations to more than 1,200 prospective students,…

  20. Technical Assistance and Training from the Document Design Project. Final Report.

    ERIC Educational Resources Information Center

    American Institutes for Research, Washington, DC.

    Contained in this report is a description of the technical assistance and training phase of the Document Design Project, a program funded by the National Institute of Education and intended to address and correct the readability problems posed by public documents. The first section of the report provides background material on the assistance and…

  1. Determining and Validating Barriers to Post-Secondary Vocational, Technical and Adult Education Programs in Wisconsin. Final Report.

    ERIC Educational Resources Information Center

    Farning, Max; Borden, Sally

    A consortium of five Wisconsin Vocational, Technical, and Adult Education (VTAE) Districts (Gateway, Indianhead, Mid-State, Milwaukee, and Southwest) were utilized to identify, verify, and alleviate barriers to enrollment. A VTAE survey in 1976 identified six major reasons for individuals' failing to attend school after indicating an interest in…

  2. Sociometric Clique Identification. Final Report.

    ERIC Educational Resources Information Center

    Kadushin, Charles

    This report consists of four parts. The first part is a non-technical summary of the basic problem and an attempted solution. The second part is a technical review of the literature and a description of the basic algorithm used in the solution. The third part describes the use of the Sociogram System. The fourth part describes the use of CHAIN, a…

  3. An Assessment of Workplace Skills Acquired by Students of Vocational and Technical Education Institutions

    ERIC Educational Resources Information Center

    Bakar, Ab Rahim; Mohamed, Shamsiah; Hamzah, Ramlah

    2013-01-01

    This study was performed to identify the employability skills of technical students from the Industrial Training Institutes (ITI) and Indigenous People's Trust Council (MARA) Skills Training Institutes (IKM) in Malaysia. The study sample consisted of 850 final year trainees of IKM and ITI. The sample was chosen by a random sampling procedure from…

  4. Wisconsin Technical College System Board Equity Staff Development Workshops and Services--Phase IV. Final Report.

    ERIC Educational Resources Information Center

    Baldus, Lorayne

    A staff development program on gender equity was conducted for personnel in Wisconsin's technical colleges using the train-the-trainer method. The training took two approaches: a class for college personnel and career challenge training for project directors of single parent and displaced homemaker grants. The inservice class resulted in increased…

  5. A Technical, User and Cost Comparison Study of Microfiche Duplicate Film Material. Final Report.

    ERIC Educational Resources Information Center

    Prevel, James J.

    A technical, user and cost comparison study was undertaken to provide the Educational Resources Information Clearinghouse (ERIC) staff with data on silver halide, diazo, and vesicular type films for microfiche duplication. This information will allow ERIC to determine if diazo and/or vesicular films should be considered in producing ERIC duplicate…

  6. Understanding a technical language: A schema-based approach

    NASA Technical Reports Server (NTRS)

    Falzon, P.

    1984-01-01

    Workers in many job categories tend to develop technical languages, which are restricted subjects of natural language. A better knowledge of these retrictions provides guidelines for the design of the restricted languages of interactive systems. Accordingly, a technical language used by air-traffic controllers in their communications with pilots was studied. A method of analysis is presented that allows the schemata underlying each category of messages to be identified. This schematic knowledge was implemented in programs, which assume that the goal-oriented aspect of technical languages (and particularly the restricted domain of discourse) limits the processes and the data necessary in order to understand the messages (monosemy, limited vocabulary, evocation of the schemata by some command words, absence of syntax). The programs can interpret, and translate into sequences of action, the messages emitted by the controllers.

  7. Highly multiplexed targeted DNA sequencing from single nuclei.

    PubMed

    Leung, Marco L; Wang, Yong; Kim, Charissa; Gao, Ruli; Jiang, Jerry; Sei, Emi; Navin, Nicholas E

    2016-02-01

    Single-cell DNA sequencing methods are challenged by poor physical coverage, high technical error rates and low throughput. To address these issues, we developed a single-cell DNA sequencing protocol that combines flow-sorting of single nuclei, time-limited multiple-displacement amplification (MDA), low-input library preparation, DNA barcoding, targeted capture and next-generation sequencing (NGS). This approach represents a major improvement over our previous single nucleus sequencing (SNS) Nature Protocols paper in terms of generating higher-coverage data (>90%), thereby enabling the detection of genome-wide variants in single mammalian cells at base-pair resolution. Furthermore, by pooling 48-96 single-cell libraries together for targeted capture, this approach can be used to sequence many single-cell libraries in parallel in a single reaction. This protocol greatly reduces the cost of single-cell DNA sequencing, and it can be completed in 5-6 d by advanced users. This single-cell DNA sequencing protocol has broad applications for studying rare cells and complex populations in diverse fields of biological research and medicine.

  8. Electronic data generation and display system

    NASA Technical Reports Server (NTRS)

    Wetekamm, Jules

    1988-01-01

    The Electronic Data Generation and Display System (EDGADS) is a field tested paperless technical manual system. The authoring provides subject matter experts the option of developing procedureware from digital or hardcopy inputs of technical information from text, graphics, pictures, and recorded media (video, audio, etc.). The display system provides multi-window presentations of graphics, pictures, animations, and action sequences with text and audio overlays on high resolution color CRT and monochrome portable displays. The database management system allows direct access via hierarchical menus, keyword name, ID number, voice command or touch of a screen pictoral of the item (ICON). It contains operations and maintenance technical information at three levels of intelligence for a total system.

  9. The optimal design of stepped wedge trials with equal allocation to sequences and a comparison to other trial designs.

    PubMed

    Thompson, Jennifer A; Fielding, Katherine; Hargreaves, James; Copas, Andrew

    2017-12-01

    Background/Aims We sought to optimise the design of stepped wedge trials with an equal allocation of clusters to sequences and explored sample size comparisons with alternative trial designs. Methods We developed a new expression for the design effect for a stepped wedge trial, assuming that observations are equally correlated within clusters and an equal number of observations in each period between sequences switching to the intervention. We minimised the design effect with respect to (1) the fraction of observations before the first and after the final sequence switches (the periods with all clusters in the control or intervention condition, respectively) and (2) the number of sequences. We compared the design effect of this optimised stepped wedge trial to the design effects of a parallel cluster-randomised trial, a cluster-randomised trial with baseline observations, and a hybrid trial design (a mixture of cluster-randomised trial and stepped wedge trial) with the same total cluster size for all designs. Results We found that a stepped wedge trial with an equal allocation to sequences is optimised by obtaining all observations after the first sequence switches and before the final sequence switches to the intervention; this means that the first sequence remains in the control condition and the last sequence remains in the intervention condition for the duration of the trial. With this design, the optimal number of sequences is [Formula: see text], where [Formula: see text] is the cluster-mean correlation, [Formula: see text] is the intracluster correlation coefficient, and m is the total cluster size. The optimal number of sequences is small when the intracluster correlation coefficient and cluster size are small and large when the intracluster correlation coefficient or cluster size is large. A cluster-randomised trial remains more efficient than the optimised stepped wedge trial when the intracluster correlation coefficient or cluster size is small. A cluster-randomised trial with baseline observations always requires a larger sample size than the optimised stepped wedge trial. The hybrid design can always give an equally or more efficient design, but will be at most 5% more efficient. We provide a strategy for selecting a design if the optimal number of sequences is unfeasible. For a non-optimal number of sequences, the sample size may be reduced by allowing a proportion of observations before the first or after the final sequence has switched. Conclusion The standard stepped wedge trial is inefficient. To reduce sample sizes when a hybrid design is unfeasible, stepped wedge trial designs should have no observations before the first sequence switches or after the final sequence switches.

  10. Chemical Frustration. A Design Principle for the Discovery of New Complex Alloy and Intermetallic Phases, Final Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fredrickson, Daniel C

    2015-06-23

    Final technical report for "Chemical Frustration: A Design Principle for the Discovery of New Complex Alloy and Intermetallic Phases" funded by the Office of Science through the Materials Chemistry Program of the Office of Basic Energy Sciences.

  11. Technical support for geopressured-geothermal activities in Louisiana. Final geological report, 1 November 1981-31 October 1982

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pilger, R.H.

    1984-06-25

    This report contains the component Site-Specific Studies-Geophysics, Diagenesis, Geochemistry. Work for Site-Specific Studies is finished, and the results presented in this report are final. Individual studies have been entered separately into the data base. (ACR)

  12. 78 FR 54566 - Energy Labeling Rule

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-05

    ... FEDERAL TRADE COMMISSION 16 CFR Part 305 RIN 3084-AB03 Energy Labeling Rule AGENCY: Federal Trade Commission. ACTION: Final rule; correction. SUMMARY: The Federal Trade Commission published a final rule on July 23, 2013 revising its Energy Labeling Rule. This document makes a technical correction to the...

  13. Wyoming Deaf/Blind Grant. Final Report.

    ERIC Educational Resources Information Center

    Whitson, Joanne B.

    This final report describes activities and accomplishments of the Wyoming Deaf-Blind Grant, a 3-year federally supported project to identify children who have deaf-blindness and to provide technical assistance in the development of educational services for these children. Major accomplishments of the project included: identification of more…

  14. Design and characterization of a 52K SNP chip for goats.

    PubMed

    Tosser-Klopp, Gwenola; Bardou, Philippe; Bouchez, Olivier; Cabau, Cédric; Crooijmans, Richard; Dong, Yang; Donnadieu-Tonon, Cécile; Eggen, André; Heuven, Henri C M; Jamli, Saadiah; Jiken, Abdullah Johari; Klopp, Christophe; Lawley, Cynthia T; McEwan, John; Martin, Patrice; Moreno, Carole R; Mulsant, Philippe; Nabihoudine, Ibouniyamine; Pailhoux, Eric; Palhière, Isabelle; Rupp, Rachel; Sarry, Julien; Sayre, Brian L; Tircazes, Aurélie; Jun Wang; Wang, Wen; Zhang, Wenguang

    2014-01-01

    The success of Genome Wide Association Studies in the discovery of sequence variation linked to complex traits in humans has increased interest in high throughput SNP genotyping assays in livestock species. Primary goals are QTL detection and genomic selection. The purpose here was design of a 50-60,000 SNP chip for goats. The success of a moderate density SNP assay depends on reliable bioinformatic SNP detection procedures, the technological success rate of the SNP design, even spacing of SNPs on the genome and selection of Minor Allele Frequencies (MAF) suitable to use in diverse breeds. Through the federation of three SNP discovery projects consolidated as the International Goat Genome Consortium, we have identified approximately twelve million high quality SNP variants in the goat genome stored in a database together with their biological and technical characteristics. These SNPs were identified within and between six breeds (meat, milk and mixed): Alpine, Boer, Creole, Katjang, Saanen and Savanna, comprising a total of 97 animals. Whole genome and Reduced Representation Library sequences were aligned on >10 kb scaffolds of the de novo goat genome assembly. The 60,000 selected SNPs, evenly spaced on the goat genome, were submitted for oligo manufacturing (Illumina, Inc) and published in dbSNP along with flanking sequences and map position on goat assemblies (i.e. scaffolds and pseudo-chromosomes), sheep genome V2 and cattle UMD3.1 assembly. Ten breeds were then used to validate the SNP content and 52,295 loci could be successfully genotyped and used to generate a final cluster file. The combined strategy of using mainly whole genome Next Generation Sequencing and mapping on a contig genome assembly, complemented with Illumina design tools proved to be efficient in producing this GoatSNP50 chip. Advances in use of molecular markers are expected to accelerate goat genomic studies in coming years.

  15. Design and Characterization of a 52K SNP Chip for Goats

    PubMed Central

    Tosser-Klopp, Gwenola; Bardou, Philippe; Bouchez, Olivier; Cabau, Cédric; Crooijmans, Richard; Dong, Yang; Donnadieu-Tonon, Cécile; Eggen, André; Heuven, Henri C. M.; Jamli, Saadiah; Jiken, Abdullah Johari; Klopp, Christophe; Lawley, Cynthia T.; McEwan, John; Martin, Patrice; Moreno, Carole R.; Mulsant, Philippe; Nabihoudine, Ibouniyamine; Pailhoux, Eric; Palhière, Isabelle; Rupp, Rachel; Sarry, Julien; Sayre, Brian L.; Tircazes, Aurélie; Jun Wang; Wang, Wen; Zhang, Wenguang

    2014-01-01

    The success of Genome Wide Association Studies in the discovery of sequence variation linked to complex traits in humans has increased interest in high throughput SNP genotyping assays in livestock species. Primary goals are QTL detection and genomic selection. The purpose here was design of a 50–60,000 SNP chip for goats. The success of a moderate density SNP assay depends on reliable bioinformatic SNP detection procedures, the technological success rate of the SNP design, even spacing of SNPs on the genome and selection of Minor Allele Frequencies (MAF) suitable to use in diverse breeds. Through the federation of three SNP discovery projects consolidated as the International Goat Genome Consortium, we have identified approximately twelve million high quality SNP variants in the goat genome stored in a database together with their biological and technical characteristics. These SNPs were identified within and between six breeds (meat, milk and mixed): Alpine, Boer, Creole, Katjang, Saanen and Savanna, comprising a total of 97 animals. Whole genome and Reduced Representation Library sequences were aligned on >10 kb scaffolds of the de novo goat genome assembly. The 60,000 selected SNPs, evenly spaced on the goat genome, were submitted for oligo manufacturing (Illumina, Inc) and published in dbSNP along with flanking sequences and map position on goat assemblies (i.e. scaffolds and pseudo-chromosomes), sheep genome V2 and cattle UMD3.1 assembly. Ten breeds were then used to validate the SNP content and 52,295 loci could be successfully genotyped and used to generate a final cluster file. The combined strategy of using mainly whole genome Next Generation Sequencing and mapping on a contig genome assembly, complemented with Illumina design tools proved to be efficient in producing this GoatSNP50 chip. Advances in use of molecular markers are expected to accelerate goat genomic studies in coming years. PMID:24465974

  16. Generation and validation of PAX7 reporter lines from human iPS cells using CRISPR/Cas9 technology.

    PubMed

    Wu, Jianbo; Hunt, Samuel D; Xue, Haipeng; Liu, Ying; Darabi, Radbod

    2016-03-01

    Directed differentiation of iPS cells toward various tissue progenitors has been the focus of recent research. Therefore, generation of tissue-specific reporter iPS cell lines provides better understanding of developmental stages in iPS cells. This technical report describes an efficient strategy for generation and validation of knock-in reporter lines in human iPS cells using the Cas9-nickase system. Here, we have generated a knock-in human iPS cell line for the early myogenic lineage specification gene of PAX7. By introduction of site-specific double-stranded breaks (DSB) in the genomic locus of PAX7 using CRISPR/Cas9 nickase pairs, a 2A-GFP reporter with selection markers has been incorporated before the stop codon of the PAX7 gene at the last exon. After positive and negative selection, single cell-derived human iPS clones have been isolated and sequenced for in-frame positioning of the reporter construct. Finally, by using a nuclease-dead Cas9 activator (dCas9-VP160) system, the promoter region of PAX7 has been targeted for transient gene induction to validate the GFP reporter activity. This was confirmed by flow cytometry analysis and immunostaining for PAX7 and GFP. This technical report provides a practical guideline for generation and validation of knock-in reporters using CRISPR/Cas9 system. Published by Elsevier B.V.

  17. ARK: Aggregation of Reads by K-Means for Estimation of Bacterial Community Composition.

    PubMed

    Koslicki, David; Chatterjee, Saikat; Shahrivar, Damon; Walker, Alan W; Francis, Suzanna C; Fraser, Louise J; Vehkaperä, Mikko; Lan, Yueheng; Corander, Jukka

    2015-01-01

    Estimation of bacterial community composition from high-throughput sequenced 16S rRNA gene amplicons is a key task in microbial ecology. Since the sequence data from each sample typically consist of a large number of reads and are adversely impacted by different levels of biological and technical noise, accurate analysis of such large datasets is challenging. There has been a recent surge of interest in using compressed sensing inspired and convex-optimization based methods to solve the estimation problem for bacterial community composition. These methods typically rely on summarizing the sequence data by frequencies of low-order k-mers and matching this information statistically with a taxonomically structured database. Here we show that the accuracy of the resulting community composition estimates can be substantially improved by aggregating the reads from a sample with an unsupervised machine learning approach prior to the estimation phase. The aggregation of reads is a pre-processing approach where we use a standard K-means clustering algorithm that partitions a large set of reads into subsets with reasonable computational cost to provide several vectors of first order statistics instead of only single statistical summarization in terms of k-mer frequencies. The output of the clustering is then processed further to obtain the final estimate for each sample. The resulting method is called Aggregation of Reads by K-means (ARK), and it is based on a statistical argument via mixture density formulation. ARK is found to improve the fidelity and robustness of several recently introduced methods, with only a modest increase in computational complexity. An open source, platform-independent implementation of the method in the Julia programming language is freely available at https://github.com/dkoslicki/ARK. A Matlab implementation is available at http://www.ee.kth.se/ctsoftware.

  18. Impact of a didactic sequence on basic Astronomy concepts for graduates in physics of online and classroom modalities

    NASA Astrophysics Data System (ADS)

    Voelzke, M. R.; Barbosa, J. I. L.

    2017-07-01

    In the transition from elementary school to high school, the topics related to astronomy are studied within the curriculae component of physics. In this context, at some point of time a Physic's teacher at this level of education will be faced with the need to work with the contents related to this science. In this way, it is important to broaden the discussion about teacher education, as well as to apply in practice the means for it. Therefore, this work has the objective to present the results, obtained by application of a questionnaire at the beginning (a pre-test), and at the final stage by a course for physics graduates of online and classroom modalities (completed with a post-test), which was organized through the Potentially Significant Teaching Units - PSTUs; and this work also presents the level of satisfaction of them in relation to the course. It is an applied and descriptive research, and the adopted technical procedures consisted of the survey and a participatory research. The data were organized in spreadsheets and the statistical analyzes were made in the sequence, with the objective of establishing comparisons between the studied groups, of their evolution of acquired knowledge and their level of satisfaction, resulting from the development in the course. The results indicate that there has been an evolution of the student's s basic knowledge with relation to the proposed topics of Astronomy in the didactic sequences, i.e., the activities developed in the administered course created a favorable atmosphere for the learning, which is therefore contributing to the initial formation of these physics teachers.

  19. Gene Editing Vectors for Studying Nicotinic Acetylcholine Receptors in Cholinergic Transmission.

    PubMed

    Peng, Can; Yan, Yijin; Kim, Veronica J; Engle, Staci E; Berry, Jennifer N; McIntosh, J Michael; Neve, Rachael L; Drenan, Ryan M

    2018-05-19

    Nicotinic acetylcholine receptors (nAChRs), prototype members of the cys-loop ligand gated ion channel family, are key mediators of cholinergic transmission in the central nervous system. Despite their importance, technical gaps exist in our ability to dissect the function of individual subunits in the brain. To overcome these barriers, we designed CRISPR/Cas9 small guide RNA sequences (sgRNAs) for production of loss-of-function alleles in mouse nAChR genes. These sgRNAs were validated in vitro via deep sequencing. We subsequently targeted candidate nAChR genes in vivo by creating herpes simplex virus (HSV) vectors delivering sgRNAs and Cas9 expression to mouse brain. Production of loss-of-function insertions or deletions (indels) by these "all-in-one" HSV vectors was confirmed using brain slice patch clamp electrophysiology coupled with pharmacological analysis. Next, we developed a scheme for cell type-specific gene editing in mouse brain. Knockin mice expressing Cas9 in a Cre-dependent manner were validated using viral microinjections and genetic crosses to common Cre-driver mouse lines. We subsequently confirmed functional Cas9 activity by targeting the ubiquitous neuronal protein, NeuN, using adeno associated virus (AAV) delivery of sgRNAs. Finally, the mouse β2 nAChR gene was successfully targeted in dopamine transporter (DAT) positive neurons via CRISPR/Cas9. The sgRNA sequences and viral vectors, including our scheme for Cre-dependent gene editing, should be generally useful to the scientific research community. These tools could lead to new discoveries related to the function of nAChRs in neurotransmission and behavioral processes. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  20. Development of Audio and Visual Media to Accompany Sequenced Instructional Programs in Physical Education for the Handicapped. Final Report. July 31, 1972.

    ERIC Educational Resources Information Center

    Avance, Lyonel D.; Carr, Dorothy B.

    Presented is the final report of a project to develop and field test audio and visual media to accompany developmentally sequenced activities appropriate for a physical education program for handicapped children from preschool through high school. Brief sections cover the following: the purposes and accomplishments of the project; the population…

  1. Control technology appendices for pollution control technical manuals. Final report, June 1982-February 1983

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1983-04-01

    The document is one of six technical handbooks prepared by EPA to help government officials granting permits to build synfuels facilities, synfuels process developers, and other interested parties. They provide technical data on waste streams from synfuels facilities and technologies capable of controlling them. Process technologies covered in the manuals include coal gasification, coal liquefaction by direct and idirect processing, and the extraction of oil from shale. The manuals offer no regulatory guidance, allowing the industry flexibility in deciding how best to comply with environmental regulations.

  2. NATO Scientific and Technical Information Service (NSTIS): functional description. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Molholm, K.N.; Blados, W.N.; Bulca, C.

    1987-08-01

    This report provides a functional description of the requirements for a NATO Scientific and Technical Information Service (NSTIS). The user requirements and much of the background information in this report were derived primarily from interviews with more than 60 NATO Headquarters staff members between 2 March and 25 March 1987. In addition, representatives of the Supreme Headquarters Applied Powers Europe (SHAPE) Technical Centre (STC), the Supreme Allied Commander Atlantic (Anti-Submarine Warfare Research) Centre (SACLANTCEN), the NATO Communications and Information Systems Agency (NACISA), The Advisory Group for Aerospace Research and Development (AGARD), the U.S. Defense Technical Information Center (DTIC), and themore » Technical Documentation Center for the Armed Forces in the Netherlands (TDCK), were interviewed, either in person or by telephone.« less

  3. A Module Experimental Process System Development Unit (MEPSDU)

    NASA Technical Reports Server (NTRS)

    1982-01-01

    Restructuring research objectives from a technical readiness demonstration program to an investigation of high risk, high payoff activities associated with producing photovoltaic modules using non-CZ sheet material is reported. Deletion of the module frame in favor of a frameless design, and modification in cell series parallel electrical interconnect configuration are reviewed. A baseline process sequence was identified for the fabrication of modules using the selected dendritic web sheet material, and economic evaluations of the sequence were completed.

  4. Job Skills Education Program. Final Technical Report.

    ERIC Educational Resources Information Center

    Florida State Univ., Tallahassee. Center for Educational Technology.

    This publication provides materials developed by a project designed to transfer a U.S. Army computer-based basic skills curriculum to applications in the vocational skills development of civilian adults. An executive summary of the final report describes the Job Skills Education Program (JSEP), which teaches academic skills that support vocational…

  5. The Vocational Education Component of the Rhode Island Educational Management Information System.

    ERIC Educational Resources Information Center

    Galamaga, Donald P.; Bartolomeo, Paul A.

    The document describes the implementation (Phase Two) of the Vocational Educational module--one component of an educational management information system. Phase Two entails the technical effort of final system design, final output specifications, edit specifications, system software selection, computer programing, systems documentation and the…

  6. Services for Children with Deaf-Blindness in Louisiana. Final Performance Report.

    ERIC Educational Resources Information Center

    Teddlie, Charles

    This final report describes activities and accomplishments of the Services for Children with Deaf-Blindness project, a 1-year federally supported project in Louisiana to improve identification and curriculum for these children by providing technical assistance and training to parents, school systems, and agency personnel. Project activities…

  7. Evaluating Potential Exposures to Ecological Receptors Due to Transport of Hydrophobic Organic Contaminants in Subsurface Systems (Final Report)

    EPA Science Inventory

    EPA's Ecological Risk Assessment Support Center (ERASC) announced the release of the final report, Evaluating Potential Exposures to Ecological Receptors Due to Transport of Hydrophobic Organic Contaminants in Subsurface Systems. This technical paper recommends several ty...

  8. 78 FR 12955 - Final Requirements, Definitions, and Selection Criteria-Native American Career and Technical...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-26

    ... has established under the Elementary and Secondary Education Act of 1965, as amended (20 U.S.C. 6301..., cooperative education, school-based enterprises, entrepreneurship, community service learning, and job... DEPARTMENT OF EDUCATION 34 CFR Chapter IV [Docket ID ED-2012-OVAE-0053] Final Requirements...

  9. 78 FR 73083 - Compassionate Release

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-05

    ... district in which the inmate was sentenced; and the final decision is subject to the general supervision... rule making a technical change to the regulations on February 28, 2013 (78 FR 13478). We now withdraw... the inmate was sentenced; and (2) clarifying that the final decision is subject to the general...

  10. SIAM Conference on Geometric Design and Computing. Final Technical Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    None

    2002-03-11

    The SIAM Conference on Geometric Design and Computing attracted 164 domestic and international researchers, from academia, industry, and government. It provided a stimulating forum in which to learn about the latest developments, to discuss exciting new research directions, and to forge stronger ties between theory and applications. Final Report

  11. High/Scope Outreach Project. Final Report. October 1, 1983-September 30, 1984.

    ERIC Educational Resources Information Center

    High/Scope Educational Research Foundation, Ypsilanti, MI.

    The final report reviews accomplishments of an outreach project designed to provide technical assistance and training to early childhood programs for handicapped children. The project features the Cognitively Oriented Preschool Curriculum, a developmental approach based on Piagetian theory and explained to build on the child's accomplishments. A…

  12. Comparison of Final Grades between a Traditional Classroom and Learning Community Classroom at Northeast Wisconsin Technical College.

    ERIC Educational Resources Information Center

    Van Beek, Dianne

    This study examines courses in the marketing program at Northeast Wisconsin Technical College to compare the academic performance of students in traditional and learning community classroom settings. Two sections of students, a traditional 17-week course and a block scheduled 5-week course, served as the sample in the study. The block scheduling…

  13. Classified Information and Technical Libraries. Final Report. Army Technical Library Improvement Studies (ATLIS), Report No. 11.

    ERIC Educational Resources Information Center

    Luger, Herbert P.; Booser, Ronald J.

    A survey of the literature in the last ten years and interviews with library and security personnel indicated: (1)the problems of handling classified information in libraries have been scanted; (2) there is wide divergence in policies and practices of disseminating such materials; (3)interlibrary cooperation with respect to classified holdings is…

  14. Field Testing Competency-Based Vocational Education Student Learning Guides Developed at Ridge Vocational-Technical Center. From August 1, 1981 to June 30, 1982. Final Report.

    ERIC Educational Resources Information Center

    Andreyka, Robert E.

    This project's main objective was to field test competency-based vocational education (CBVE) student learning guides developed during 1979-1981 at Ridge Vocational-Technical Center (RVTC) (Florida). The learning guides were for six programs: clerical occupations, cosmetology, heavy duty truck/bus mechanics, industrial electricity, masonry, and…

  15. 78 FR 17871 - Changes To Implement the Technical Corrections to the Leahy-Smith America Invents Act as to Inter...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-25

    ... rule eliminates the nine-month ``dead zone'' for filing an inter partes review petition challenging a..., section 1(d) of the AIA Technical Corrections Act and this final rule eliminate the nine-month ``dead zone... the nine-month ``dead zone'' as to first-to-invent patents and reissue patents. Costs and Benefits...

  16. Research in Fiber Optics: Implications for Fiber Optics in Vocational-Technical Education. Final Report 1984-85.

    ERIC Educational Resources Information Center

    Bergen County Vocational-Technical High School, Hackensack, NJ.

    This project was conducted to determine the vocational, technical, and scientific skills and knowledge needed to work with the fiber optics applications that are in all areas of technology. A research assistant was hired by the project director to collect data and develop a research base for the project. Information was gathered through a…

  17. The California spotted owl: a technical assessment of its current status

    Treesearch

    Jared Verner; Kevin S. McKelvey; Barry R. Noon; R. J. Gutierrez; Gordon I. Jr. Gould; Thomas W. Beck

    1992-01-01

    This report is based an the Final Repart submitted on May 8, 1992 by the Technical Assessment Team to the interagency Steering Committee for the California Spotted Owl Assessment. The 13 chapters cover the assessment of the current status of the California spotted owl, its biology and habitat use, and forests where the subspecies occurs in the Sierra Nevada and...

  18. Unified Technical Concepts - Phase I. (Modularizing Instructional Materials Using Applications of Technical Concepts). Final Report.

    ERIC Educational Resources Information Center

    Technical Education Research Center, Waco, TX.

    To evolve a new methodology and system for teaching physics to students aspiring to become (or to become more competent as) technicians in a variety of technologies, this research and development effort was initiated. The project's thesis stemmed from a notion that the study of physics would be more accepted and assimilated by students if concepts…

  19. Establishment of the Center for Advanced Separation Technologies

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Christopher E. Hull

    2006-09-30

    This Final Technical Report covers the eight sub-projects awarded in the first year and the five projects awarded in the second year of Cooperative Agreement DE-FC26-01NT41091: Establishment of the Center for Advanced Separation Technologies. This work is summarized in the body of the main report: the individual sub-project Technical Progress Reports are attached as Appendices.

  20. Moka with Ristretto

    DTIC Science & Technology

    2017-11-01

    MOKA WITH RISTRETTO ASSURED INFORMATION SECURITY, INC. NOVEMBER 2017 FINAL TECHNICAL REPORT APPROVED FOR PUBLIC RELEASE; DISTRIBUTION UNLIMITED...STINFO COPY AIR FORCE RESEARCH LABORATORY INFORMATION DIRECTORATE AFRL-RI-RS-TR-2017-223  UNITED STATES AIR FORCE  ROME, NY 13441 AIR FORCE...report is available to the general public, including foreign nations. Copies may be obtained from the Defense Technical Information Center (DTIC

  1. Development of a Screening Methodology for Entry into Medical Technical Training Courses. Final Report for Period October 1975-April 1977.

    ERIC Educational Resources Information Center

    Leisey, Sandra A.; Guinn, Nancy

    At the request of the Air Force School of Aviation Medicine, a project was initiated to evaluate the current screening process used for entry into three medical technical training courses: Aeromedical Specialist, Environmental Health Specialist, and Physiological Training Specialist. A sample of 1,003 students were administered the General…

  2. Summer Institute to Train Data Processing Teachers for the New Oklahoma State-Wide Computer Science System, Phase II. Final Report.

    ERIC Educational Resources Information Center

    Tuttle, Francis

    Twenty-three instructors participated in an 8-week summer institute to develop their technical competency to teach the second year of a 2-year Technical Education Computer Science Program. Instructional material covered the following areas: (1) compiler languages and systems design, (2) cost studies, (3) business organization, (4) advanced…

  3. Technical bases and guidance for the use of composite soil sampling for demonstrating compliance with radiological release criteria

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Vitkus, Timothy J.

    2012-04-24

    This guidance provides information on methodologies and the technical bases that licensees should consider for incorporating composite sampling strategies into final status survey (FSS) plans. In addition, this guidance also includes appropriate uses of composite sampling for generating the data for other decommissioning site investigations such as characterization or other preliminary site investigations.

  4. FIELD CHECK MANUAL FOR LANGUAGE LABORATORIES, A SERIES OF TESTS WHICH A NON-TECHNICAL PERSON CAN CONDUCT TO VERIFY SPECIFICATIONS.

    ERIC Educational Resources Information Center

    GRITTNER, FRANK; PAVLAT, RUSSELL

    IN ORDER TO ASSIST NON-TECHNICAL PEOPLE IN SCHOOLS TO CONDUCT A FIELD CHECK OF LANGUAGE LABORATORY EQUIPMENT BEFORE THEY MAKE FINAL PAYMENTS, THIS MANUAL OFFERS CRITERIA, TESTS, AND METHODS OF SCORING THE QUALITY OF THE EQUIPMENT. CHECKLISTS ARE PROVIDED FOR EVALUATING CONSOLE FUNCTIONS, TAPE RECORDERS, AMPLIFIERS, SOUND QUALITY (INCLUDING…

  5. Organic and Hybrid Organic Solid-State Photovoltaic Materials and Devices

    DTIC Science & Technology

    2014-03-06

    Microscopy Research, 2012, 7, 158-169. Organic photovoltaic materials, hybrid organic devices, solar cells 6 1 FINAL TECHNICAL REPORT 1... hybrids have potential applications in solar cells and may thus provide mobile energy sources for aircraft and soldier technologies. Modeling and...modeling and simulation developed in this project are encouraging further development. 2. Technical Activities Hybrid organic solar cells are an

  6. Space station automation study: Autonomous systems and assembly, volume 2

    NASA Technical Reports Server (NTRS)

    Bradford, K. Z.

    1984-01-01

    This final report, prepared by Martin Marietta Denver Aerospace, provides the technical results of their input to the Space Station Automation Study, the purpose of which is to develop informed technical guidance in the use of autonomous systems to implement space station functions, many of which can be programmed in advance and are well suited for automated systems.

  7. Technical Report on Modeling for Quasispecies Abundance Inference with Confidence Intervals from Metagenomic Sequence Data

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McLoughlin, K.

    2016-01-11

    The overall aim of this project is to develop a software package, called MetaQuant, that can determine the constituents of a complex microbial sample and estimate their relative abundances by analysis of metagenomic sequencing data. The goal for Task 1 is to create a generative model describing the stochastic process underlying the creation of sequence read pairs in the data set. The stages in this generative process include the selection of a source genome sequence for each read pair, with probability dependent on its abundance in the sample. The other stages describe the evolution of the source genome from itsmore » nearest common ancestor with a reference genome, breakage of the source DNA into short fragments, and the errors in sequencing the ends of the fragments to produce read pairs.« less

  8. Real-time tracking of respiratory-induced tumor motion by dose-rate regulation

    NASA Astrophysics Data System (ADS)

    Han-Oh, Yeonju Sarah

    We have developed a novel real-time tumor-tracking technology, called Dose-Rate-Regulated Tracking (DRRT), to compensate for tumor motion caused by breathing. Unlike other previously proposed tumor-tracking methods, this new method uses a preprogrammed dynamic multileaf collimator (MLC) sequence in combination with real-time dose-rate control. This new scheme circumvents the technical challenge in MLC-based tumor tracking, that is to control the MLC motion in real time, based on real-time detected tumor motion. The preprogrammed MLC sequence describes the movement of the tumor, as a function of breathing phase, amplitude, or tidal volume. The irregularity of tumor motion during treatment is handled by real-time regulation of the dose rate, which effectively speeds up or slows down the delivery of radiation as needed. This method is based on the fact that all of the parameters in dynamic radiation delivery, including MLC motion, are enslaved to the cumulative dose, which, in turn, can be accelerated or decelerated by varying the dose rate. Because commercially available MLC systems do not allow the MLC delivery sequence to be modified in real time based on the patient's breathing signal, previously proposed tumor-tracking techniques using a MLC cannot be readily implemented in the clinic today. By using a preprogrammed MLC sequence to handle the required motion, the task for real-time control is greatly simplified. We have developed and tested the pre- programmed MLC sequence and the dose-rate regulation algorithm using lung-cancer patients breathing signals. It has been shown that DRRT can track the tumor with an accuracy of less than 2 mm for a latency of the DRRT system of less than 0.35 s. We also have evaluated the usefulness of guided breathing for DRRT. Since DRRT by its very nature can compensate for breathing-period changes, guided breathing was shown to be unnecessary for real-time tracking when using DRRT. Finally, DRRT uses the existing dose-rate control system that is provided for current linear accelerators. Therefore, DRRT can be achieved with minimal modification of existing technology, and this can shorten substantially the time necessary to establish DRRT in clinical practice.

  9. Biotechnology Program Guide.

    ERIC Educational Resources Information Center

    Georgia Univ., Athens. Dept. of Vocational Education.

    This program quide presents the biotechnology curriculum for technical institutes in Georgia. The general information section contains the following: purpose and objectives; program description, including admissions, typical job titles, and accreditation and certification; and curriculum model, including standard curriculum sequence and lists of…

  10. Civil Engineering Technology Program Guide.

    ERIC Educational Resources Information Center

    Georgia Univ., Athens. Dept. of Vocational Education.

    This program guide presents civil engineering technology curriculum for technical institutes in Georgia. The general information section contains the following: purpose and objectives; program description, including admissions, typical job titles, and accreditation and certification; and curriculum model, including standard curriculum sequence and…

  11. Genomics and breeding in food crops

    USDA-ARS?s Scientific Manuscript database

    Plant biology is in the midst of a revolution. The generation of tremendous volumes of sequence information introduce new technical challenges into plant biology and agriculture. The relatively new field of bioinformatics addresses these challenges by utilizing efficient data management strategies;...

  12. Weight Regain, But Not Weight Loss, Is Related to Competitive Success in Real-life Mixed Martial Arts Competition.

    PubMed

    Coswig, Victor Silveira; Miarka, Bianca; Pires, Daniel Alvarez; da Silva, Levy Mendes; Bartel, Charles; Del Vecchio, Fabrício Boscolo

    2018-05-14

    We aimed to describe the nutritional and behavioural strategies for rapid weight loss (RWL), investigate the effects of RWL and weight regain (WRG) in winners and losers and verify mood state and technical-tactical/time-motion parameters in Mixed Martial Arts (MMA). The sample consisted of MMA athletes after a single real match and was separated into two groups: Winners (n=8, age: 25.4±6.1yo., height: 173.9±0.2cm, habitual body mass (BM): 89.9±17.3kg) and Losers (n=7, age: 24.4±6.8yo., height: 178.4±0.9cm, habitual BM: 90.8±19.5kg). Both groups exhibited RWL and WRG, verified their macronutrient intake, underwent weight and height assessments and completed two questionnaires (POMS and RWL) at i) 24 h before weigh-in, ii) weigh-in, iii) post-bout and iv) during a validated time-motion and technical-tactical analysis during the bout. Variance analysis, repeated measures and a logistic regression analysis were used. The main results showed significant differences between the time points in terms of total caloric intake as well as carbohydrate, protein and lipid ingestion. Statistical differences in combat analysis were observed between the winners and losers in terms of high-intensity relative time [58(10;98) s and 32(1;60) s, respectively], lower limb sequences [3.5(1.0;7.5) sequences and 1.0(0.0;1.0) sequences, respectively], and ground and pound actions [2.5(0.0;4.5) actions and 0.0(0.0;0.5) actions, respectively], and logistic regression confirmed the importance of high-intensity relative time and lower limb sequences on MMA performance. RWL and WRG strategies were related to technical-tactical and time-motion patterns as well as match outcomes. Weight management should be carefully supervised by specialized professionals to reduce health risks and raise competitive performance.

  13. NESC Independent Review of the Mars Reconnaissance Orbiter (MRO) Contamination Thermal/Vacuum (T/V) Anomaly Technical Consultation Report

    NASA Technical Reports Server (NTRS)

    Sutter, James K.; Leidecker, Henning W.; Panda, Binayak; Piascik, Robert S.; Muirhead, Brian K.; Peeler, Debra

    2009-01-01

    The NESC eras requested by the NASA Jet Propulsion Laboratory (JPL) to conduct an independent review of the Mars Reconnaissance Orbiter (MRO) Thermal/Vacuum (T/V) Anomaly Assessment. Because the anomaly resulted in the surface contamination of the MRO, selected members of the Materials Super Problem Resolution Team (SPRT) and the NASA technical community having technical expertise relative to contamination issues were chosen for the independent review. The consultation consisted of a review of the MRO Project's reported response to the assessment findings, a detailed review of JPL technical assessment final report, and detailed discussions with the JPL assessment team relative to their findings.

  14. Final Technical Report for DE-FG02-98ER45737

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ade, Harald W.

    This document represents the cumulative, final technical report for Grant No. DE-FG02- 98ER45737, the title of which has changed with each funding period, but the research pursued is within a coherent overall theme of methods and technique developments that exploit contrast at the carbon absorption edge to characterize complex organic materials and the use of these synchrotron radiation-based methods for important research challenges in polymer physics and Materials Science. The last three funding periods focused on organic devices and in particular organic solar cells (OSCs), owing to their extra-ordinarily complex morphology, yet high potential as a cheap and printable power-conversionmore » technology.« less

  15. Microfluidic single-cell whole-transcriptome sequencing.

    PubMed

    Streets, Aaron M; Zhang, Xiannian; Cao, Chen; Pang, Yuhong; Wu, Xinglong; Xiong, Liang; Yang, Lu; Fu, Yusi; Zhao, Liang; Tang, Fuchou; Huang, Yanyi

    2014-05-13

    Single-cell whole-transcriptome analysis is a powerful tool for quantifying gene expression heterogeneity in populations of cells. Many techniques have, thus, been recently developed to perform transcriptome sequencing (RNA-Seq) on individual cells. To probe subtle biological variation between samples with limiting amounts of RNA, more precise and sensitive methods are still required. We adapted a previously developed strategy for single-cell RNA-Seq that has shown promise for superior sensitivity and implemented the chemistry in a microfluidic platform for single-cell whole-transcriptome analysis. In this approach, single cells are captured and lysed in a microfluidic device, where mRNAs with poly(A) tails are reverse-transcribed into cDNA. Double-stranded cDNA is then collected and sequenced using a next generation sequencing platform. We prepared 94 libraries consisting of single mouse embryonic cells and technical replicates of extracted RNA and thoroughly characterized the performance of this technology. Microfluidic implementation increased mRNA detection sensitivity as well as improved measurement precision compared with tube-based protocols. With 0.2 M reads per cell, we were able to reconstruct a majority of the bulk transcriptome with 10 single cells. We also quantified variation between and within different types of mouse embryonic cells and found that enhanced measurement precision, detection sensitivity, and experimental throughput aided the distinction between biological variability and technical noise. With this work, we validated the advantages of an early approach to single-cell RNA-Seq and showed that the benefits of combining microfluidic technology with high-throughput sequencing will be valuable for large-scale efforts in single-cell transcriptome analysis.

  16. Utility of NIST Whole-Genome Reference Materials for the Technical Validation of a Multigene Next-Generation Sequencing Test.

    PubMed

    Shum, Bennett O V; Henner, Ilya; Belluoccio, Daniele; Hinchcliffe, Marcus J

    2017-07-01

    The sensitivity and specificity of next-generation sequencing laboratory developed tests (LDTs) are typically determined by an analyte-specific approach. Analyte-specific validations use disease-specific controls to assess an LDT's ability to detect known pathogenic variants. Alternatively, a methods-based approach can be used for LDT technical validations. Methods-focused validations do not use disease-specific controls but use benchmark reference DNA that contains known variants (benign, variants of unknown significance, and pathogenic) to assess variant calling accuracy of a next-generation sequencing workflow. Recently, four whole-genome reference materials (RMs) from the National Institute of Standards and Technology (NIST) were released to standardize methods-based validations of next-generation sequencing panels across laboratories. We provide a practical method for using NIST RMs to validate multigene panels. We analyzed the utility of RMs in validating a novel newborn screening test that targets 70 genes, called NEO1. Despite the NIST RM variant truth set originating from multiple sequencing platforms, replicates, and library types, we discovered a 5.2% false-negative variant detection rate in the RM truth set genes that were assessed in our validation. We developed a strategy using complementary non-RM controls to demonstrate 99.6% sensitivity of the NEO1 test in detecting variants. Our findings have implications for laboratories or proficiency testing organizations using whole-genome NIST RMs for testing. Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

  17. ddClone: joint statistical inference of clonal populations from single cell and bulk tumour sequencing data.

    PubMed

    Salehi, Sohrab; Steif, Adi; Roth, Andrew; Aparicio, Samuel; Bouchard-Côté, Alexandre; Shah, Sohrab P

    2017-03-01

    Next-generation sequencing (NGS) of bulk tumour tissue can identify constituent cell populations in cancers and measure their abundance. This requires computational deconvolution of allelic counts from somatic mutations, which may be incapable of fully resolving the underlying population structure. Single cell sequencing (SCS) is a more direct method, although its replacement of NGS is impeded by technical noise and sampling limitations. We propose ddClone, which analytically integrates NGS and SCS data, leveraging their complementary attributes through joint statistical inference. We show on real and simulated datasets that ddClone produces more accurate results than can be achieved by either method alone.

  18. Environmental information acquisition and maintenance techniques: reference guide. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Riggins, R.E.; Young, V.T.; Goran, W.D.

    1980-08-01

    This report provides a guide to techniques for collecting, using and maintaining data about each of the 13 environmental technical specialties in the Environmental Impact Computer System (EICS). The technical specialties are: (1) ecology, (2) environmental health, (3) air, (4) surface water, (5) ground water, (6) sociology, (7) economics, (8) earth science, (9) land use, (10) noise, (11) transportation, (12) aesthetics, and (13) energy and resource conservation. Acquisition techniques are classified by the following general categories: (1) secondary data, (2) remote sensing, (3) mathematical modeling, (4) field work, (5) mapping/maps and (6) expert opinion. A matrix identifies the most appropriatemore » techniques for collecting information on the EICS technical specialties. After selecting a method, the user may read an abstract of the report explaining that technique, and may also wish to obtain the original document for detailed information about applying the technique. Finally, this report offers guidelines on storing environmental information for future use, and on presenting that information effectively in environmental documents.« less

  19. NEET-AMM Final Technical Report on Laser Direct Manufacturing (LDM) for Nuclear Power Components

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Anderson, Scott; Baca, Georgina; O'Connor, Michael

    2015-12-31

    Final technical report summarizes the program progress and technical accomplishments of the Laser Direct Manufacturing (LDM) for Nuclear Power Components project. A series of experiments varying build process parameters (scan speed and laser power) were conducted at the outset to establish the optimal build conditions for each of the alloys. Fabrication was completed in collaboration with Quad City Manufacturing Laboratory (QCML). The density of all sample specimens was measured and compared to literature values. Optimal build process conditions giving fabricated part densities close to literature values were chosen for making mechanical test coupons. Test coupons whose principal axis is onmore » the x-y plane (perpendicular to build direction) and on the z plane (parallel to build direction) were built and tested as part of the experimental build matrix to understand the impact of the anisotropic nature of the process.. Investigations are described 316L SS, Inconel 600, 718 and 800 and oxide dispersion strengthed 316L SS (Yttria) alloys.« less

  20. An approach to developing independent learning and non-technical skills amongst final year mining engineering students

    NASA Astrophysics Data System (ADS)

    Knobbs, C. G.; Grayson, D. J.

    2012-06-01

    There is mounting evidence to show that engineers need more than technical skills to succeed in industry. This paper describes a curriculum innovation in which so-called 'soft' skills, specifically inter-personal and intra-personal skills, were integrated into a final year mining engineering course. The instructional approach was designed to promote independent learning and to develop non-technical skills, essential for students on the threshold of becoming practising engineers. Three psychometric tests were administered at the beginning of the course to make students aware of their own and their classmates' characteristics. Substantial prescribed reading assignments preceded weekly group discussions. Several projects during the course required team work skills and application of content knowledge to real-world contexts. Results obtained from students' reflection papers, assignments related to 'soft' skills and end of course evaluations suggest that students' appreciation of the need for these skills, as well as their own perceived competence, increased during the course. Their ability to function as independent learners also increased.

  1. Key team physical and technical performance indicators indicative of team quality in the soccer Chinese super league.

    PubMed

    Yang, Gai; Leicht, Anthony S; Lago, Carlos; Gómez, Miguel-Ángel

    2018-01-01

    The aim of this study was to identify the key physical and technical performance variables related to team quality in the Chinese Super League (CSL). Teams' performance variables were collected from 240 matches and analysed via analysis of variance between end-of-season-ranked groups and multinomial logistic regression. Significant physical performance differences between groups were identified for sprinting (top-ranked group vs. upper-middle-ranked group) and total distance covered without possession (upper and upper-middle-ranked groups and lower-ranked group). For technical performance, teams in the top-ranked group exhibited a significantly greater amount of possession in opponent's half, number of entry passes in the final 1/3 of the field and the Penalty Area, and 50-50 challenges than lower-ranked teams. Finally, time of possession increased the probability of a win compared with a draw. The current study identified key performance indicators that differentiated end-season team quality within the CSL.

  2. [NASA/DOD Aerospace Knowledge Diffusion Research Project. Report 1, part 1:] Technical communications in aeronautics: Results of an exploratory study

    NASA Technical Reports Server (NTRS)

    Pinelli, Thomas E.; Glassman, Myron; Oliu, Walter E.; Barclay, Rebecca O.

    1989-01-01

    A study was undertaken that explored several aspects of technical communications in aeronautics. The study, which utilized survey research in the form of a self-administered questionnaire, was sent to 2,000 randomly selected members of the American Institute of Aeronautics and Astronautics. Six hundred and six usable questionnaires (30.3 percent) were received by the established cut off date. The study had five objectives. The first was to solicit the opinions of aeronautical engineers and scientists regarding the importance of technical communications to their profession; second, to determine their use and production of technical communications; third, to seek their views on the content of an undergraduate course in technical communications; fourth, to determine their use of libraries/technical information centers; and finally, to determine the use and importance of computer and information technology to them. The findings add considerable information to the knowledge of technical communications practices among aeronautical engineers and scientists and reinforce some of the conventional wisdom about technical communications and question other widely-held notions.

  3. Technical memo : project 0-6132 (task 6).

    DOT National Transportation Integrated Search

    2010-04-01

    The attached memo presents construction information on the test sections placed by Madden Construction on US 59 on March 26th 2010. The memo includes details of the mix design, shows photographs of the construction sequence, lab test data on the Cont...

  4. High-Throughput Sequencing: A Roadmap Toward Community Ecology

    PubMed Central

    Poisot, Timothée; Péquin, Bérangère; Gravel, Dominique

    2013-01-01

    High-throughput sequencing is becoming increasingly important in microbial ecology, yet it is surprisingly under-used to generate or test biogeographic hypotheses. In this contribution, we highlight how adding these methods to the ecologist toolbox will allow the detection of new patterns, and will help our understanding of the structure and dynamics of diversity. Starting with a review of ecological questions that can be addressed, we move on to the technical and analytical issues that will benefit from an increased collaboration between different disciplines. PMID:23610649

  5. Feasibility of Computer Processing of Technical Information on the Design of Instructional Systems. Final Report for the Period 1 July 1972 through 31 March 1973.

    ERIC Educational Resources Information Center

    Scheffler, F. L.; And Others

    A feasibility study examined the capability of a computer-based system's handling of technical information pertinent to the design of instructional systems. Structured interviews were held to assess the information needs of both researchers and practitioners and an investigation was conducted of 10 computer-based information storage and retrieval…

  6. Task Force on the Role of General Education in Associate Science Degree Programs. Final Report and Recommendations.

    ERIC Educational Resources Information Center

    Weyers, Lori; Langerman, Philip

    In 1989-90, the General Education Task Force of the Wisconsin Technical College System (WTCS) was convened to determine the role of the general education curriculum in the attainment of skills that enhance the likelihood of success among technical college graduates in their careers, homes and communities. The Task Force consisted of at least one…

  7. Adlai E. Stevenson High School, Bilingual Academic and Technical Education for Youth Program. O.E.E. Evaluation Report, 1982-1983.

    ERIC Educational Resources Information Center

    Keyes, Jose Luis; Collins, Carla

    The Bilingual Academic and Technical Education for Youth (BATEY) Program completed its third and final year of funding at Adlai E. Stevenson High School, Bronx, New York. In 1982-83, the program offered bilingual instruction and support services to approximately 300 Hispanic students of limited English proficiency in grades 9-12. Although the…

  8. 77 FR 57186 - Technical Report on Fatality Risk, Mass, and Footprint of Model Year 2000-2007 Passenger Cars and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ...-0152] Technical Report on Fatality Risk, Mass, and Footprint of Model Year 2000-2007 Passenger Cars and..., and Footprint in Model Year 2000-2007 Passenger Cars and LTVs--Final Report. DATES: Comments must be... comprised MY 2000-2007 cars and LTVs in CY 2002-2008 crashes. Fatality rates were derived from FARS data, 13...

  9. A Planning Study for the Conversion of Recorded Books and Magazines from Rigid Discs to Cassettes and Flexible Discs. Final Report.

    ERIC Educational Resources Information Center

    Innovative Systems Research, Inc., Pennsauken, NJ.

    Twelve different conversion plans were delineated and evaluated in the performance of this study effort. An indepth technical, financial, and cost/benefit analysis was included. One plan was determined to be distinctively more cost-effective than any of the other plans because it provided a technically feasible system that assured the maximum…

  10. Technical Feasibility Study of an Effective Low-Toxicity Obscurant Material

    DTIC Science & Technology

    2012-08-01

    2. REPORT TYPE FINAL 3. DATES COVERED (From - To) 04/2011 – 07/2012 4 . TITLE AND SUBTITLE 5a. CONTRACT NUMBER W912HQ-11-C-0034 Technical...6 4 Work Package 3 – Laboratory-scale tests...chemistry to sea-salt aerosol. 4 The major component of sea-salt is sodium chloride. Sodium chloride can be very simply generated by the combustion

  11. The Attitudes of the Final Students of Technical Education Faculty at Kocaeli University towards Their Faculty's Closing Down

    ERIC Educational Resources Information Center

    Ira, Nejat

    2015-01-01

    The purpose of the research is to identify the attitudes of the students at Kocaeli University towards closing down of Technical Education Faculty. These faculties were decided to be closed down in 2009. Since then, both the students and the instructors have been in ambiguity about their future careers. This situation has also affected them…

  12. A Proposed Modified Technical Order System and Its Impact on Maintenance, Personnel and Training. Final Report for Period December 1974-September 1975.

    ERIC Educational Resources Information Center

    Foley, John P., Jr.

    The document presents immediate and long range plans for the systematic injection of Symbolic Integrated Maintenance Systems (SIMS) and Fully Proceduralized Job Performance Aids (FPJPA) materials into the current technical order system and the inclusion of job-oriented training matched to FPJPA into the training system of the Air Force. The text…

  13. Advanced Authoring Technologies, Capabilities and Opportunities (Technologies avancees d’authoring, possibilites et opportunites)

    DTIC Science & Technology

    2011-02-01

    NORTH ATLANTIC TREATY ORGANISATION RESEARCH AND TECHNOLOGY ORGANISATION AC/323(HFM-129)TP/349 www.rto.nato.int RTO TECHNICAL REPORT TR...Final Report of Task Group HFM-129. Published February 2011 Distribution and Availability on Back Cover...NORTH ATLANTIC TREATY ORGANISATION RESEARCH AND TECHNOLOGY ORGANISATION AC/323(HFM-129)TP/349 www.rto.nato.int RTO TECHNICAL REPORT TR-HFM

  14. The Ability of Standardized Test Instruments to Differentiate Membership in Different Vocational-Technical Curricula. Project MINI-SCORE, Final Technical Report.

    ERIC Educational Resources Information Center

    Pucel, David J.; And Others

    Using post-secondary vocational education students as the populations, these two sub-studies of the Project MINI-SCORE sought to determine the extent to which pre-enrollment standardized test data can be used to predict vocational success. For the purpose of the study, vocational success was defined either as successful graduation or successful…

  15. Learning from Experience Case Studies of the Hyper-X Project

    NASA Technical Reports Server (NTRS)

    Peebles, Curtis

    2009-01-01

    The Hyper-X project (X-43A) provides a number of "lessons learned" which can be applied to other aerospace project. The specific areas examined were the selection of the goals of the Hyper-X. How the technical unknowns and assumptions were handled. The final lesson was the ambiguous nature of risk assessment, and how trying to remove a technical unknown can have unintended consequences.

  16. Helicopter Dynamic Performance Program. Volume 2. User’s Manual

    DTIC Science & Technology

    1980-07-01

    Technologies Corporation Stratford, Connecticut 06602 July 1980 Final Report / • ., . Approved for public release; I distribution unlimited. ; A - Prepared...for : APPLIED TECHNOLOGY LABORATORY U. S. ARMY RESEARCH AND TECHNOLOGY LABORATORIES (AVRADCOM) Fort Eustis, Va. 23604 80 8 27 D APPLIED TECHNOLOGY ... Technology Division. Technic31 review of this report was also pro- vided by Messrs. W_ A. Pleasants of Design Integration and Analysis Technical Area

  17. A Study of Placing Army Requirements on Contract

    DTIC Science & Technology

    2016-04-01

    perhaps being unable to control the external environment, has management authority over business and technical aspects of a specific program. The PM...research is the last element of the planning phase addressing both business and technical considerations. The acquisition team equips themselves with...report is the final product utilizing documented findings and supports the business strategy (DoD, Guidebook for the Acquisition of Services, 2011

  18. Comparison of Effectiveness of Collaborative Learning Methods and Traditional Methods in Physics Classes at Northern Maine Technical College.

    ERIC Educational Resources Information Center

    Overlock, Terrence H., Sr.

    To determine the effect of collaborative learning methods on the success rate of physics students at Northern Maine Technical College (NMTC), a study was undertaken to compare the mean final exam scores of a students in a physics course taught by traditional lecture/lab methods to those in a group taught by collaborative techniques. The…

  19. Impact of Entrepreneurship Education on the Entrepreneurial Intentions of Students in Technical and Vocational Education and Training Institutions (TVET) in Malaysia

    ERIC Educational Resources Information Center

    Ibrahim, Wan Nur Azlina; Bakar, Ab. Rahim; Asimiran, Soaib; Mohamed, Shamsiah; Zakaria, Noor Syamilah

    2015-01-01

    The purpose of this study was to determine the entrepreneurial intention level of vocational and technical students in Malaysia. A total of 289 final year students who were enrolled at two different TVET institutions (community colleges and National Youth and Skills Institutes) were chosen to participate in the study. The findings indicated that…

  20. A Pilot Research Project to Determine the Feasibility of Developing Health Related Occupations Curricula at Nicolet College and Technical Institute. Final Report.

    ERIC Educational Resources Information Center

    Maney, Thomas J.; Boyd, Linda

    Personal interviews with each employer of health care workers in the Nicolet Vocational, Technical and Adult Education (VTAE) District were conducted to determine: (1) the extent of need for trained health occupations personnel in the district; (2) the projected need for such personnel in the future; (3) which specific health occupations currently…

  1. Final Report of Technical Assistance Provided to Guarantee Agencies with Recommendations for Further Improving Program Performance.

    ERIC Educational Resources Information Center

    Touche Ross and Co., Washington, DC.

    Technical assistance provided to loan guarantee agencies by Touche Ross and Co. under contract to the U.S. Office of Education (OE) is described. Objectives of the report are: to summarize the work performed and the results of each of the projects that were conducted; to describe certain prototype systems that were developed for the guarantee…

  2. INTEREST AND INTELLECTUAL INDICES RELATED TO SUCCESSFUL AND NON-SUCCESSFUL MALE COLLEGE STUDENTS IN TECHNICAL AND ASSOCIATE DEGREE PROGRAMS. FINAL REPORT.

    ERIC Educational Resources Information Center

    HECKER, DONALD L.; TAYLOR, RONALD G.

    THE PURPOSE OF THE PROJECT WAS TO STUDY THE DIFFERENCES AND SIMILARITIES IN INTEREST, ABILITY, AND PREVIOUS ACHIEVEMENT PATTERNS OF 941 MEN STUDENTS WHO ENROLLED IN VARIOUS TECHNICAL AND ASSOCIATE DEGREE 2-YEAR PROGRAMS AND TO DETERMINE WHICH VARIABLES COULD DIFFERENTIATE BETWEEN STUDENTS WHO COMPLETED, CHANGED TO AN ALTERNATE PROGRAM, OR WITHDREW…

  3. Sub-Project 7 of Wisconsin 553, Part F, EPDA--Industry/Exchange Program: Final Report.

    ERIC Educational Resources Information Center

    Wisconsin Univ. - Stout, Menomonie. Center for Vocational, Technical and Adult Education.

    The final report describes a study to develop a statewide teacher/industry exchange program in Wisconsin that would provide vocational-technical instructors with occupational experience opportunities. Three plans for implementing the program were developed: (A) swapping the positions of a business or industrial person with that of a vocational…

  4. 2+2+2 Dissemination Project Final Report.

    ERIC Educational Resources Information Center

    Sloane, Diana; And Others

    An overview is provided in this three-part final report of a project designed to disseminate information to assist California high schools, Regional Occupation Programs, and colleges in developing and strengthening 2+2+2 programs. Part I reviews the following project objectives: (1) hire a project coordinator and technical assistant; (2) develop a…

  5. State Compensatory Education. Final Technical Report 1975-76. Appendixes.

    ERIC Educational Resources Information Center

    Austin Independent School District, TX. Office of Research and Evaluation.

    This material consists of ten appendixes to the Final Report for 1976-77 on the implementation of the Texas state-supported compensatory education program in the Austin Independent School District as evaluated by that district's Office of Research and Evaluation. The instruments used in the evaluation process are described or presented in full,…

  6. Pacific Basin Deaf-Blind Project. State & Multi State Projects for Children with Deaf-Blindness. Final Report, 1992-1995.

    ERIC Educational Resources Information Center

    Kelly, Dotty; Guerrero, Vincent Leon

    This final report describes activities and accomplishments of the Pacific Basin Deaf-Blind Project, a 3-year federally funded project to provide technical assistance to public and private agencies, institutions, and organizations providing early intervention, educational, transitional, vocational, early identification, and related services to…

  7. An Integrated Parent-Teacher-Related Service Team Approach to Communication Intervention. Final Report.

    ERIC Educational Resources Information Center

    Stremel, Kathleen; Wilson, Rebecca

    This final report describes a federally funded 3-year project for integrating related services within educational objectives for children (ages 3-10) with dual vision and hearing impairments. A Training-Utilization model of inservice training and technical assistance was developed, implemented, and evaluated to address the communication needs of…

  8. ADAAG Review Federal Advisory Committee Recommendations for a New ADAAG. Final Report.

    ERIC Educational Resources Information Center

    Architectural and Transportation Barriers Compliance Board, Washington, DC.

    This report presents the final recommendations, in the form of a new set of guidelines, of a federal advisory committee concerning revisions to the Americans with Disabilities Act Accessibility Guidelines (ADAAG). The guidelines provide scoping and technical requirements for the design, construction, and alteration of sites, facilities, buildings,…

  9. 76 FR 78141 - Pilot, Flight Instructor, and Pilot School Certification; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-16

    ... command under IFR or in weather conditions less than the minimums prescribed for VFR. Finally, this... pilot in command under IFR or in weather conditions less than the minimums prescribed for VFR. Under... command under IFR or in weather conditions less than the minimums prescribed for VFR. In the 2009 final...

  10. 76 FR 10516 - Transfer and Reorganization of Bank Secrecy Act Regulations-Technical Amendment.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-25

    ... concerning mutual funds and the other concerning the confidentiality of a report of suspicious activity (SAR... compliance date of the mutual fund rule. Additionally, the Chapter X Final Rule contained an inadvertent..., 2010, FinCEN issued a final rule to include mutual funds within the general definition of ``financial...

  11. Providing Computer-Based Information Services to an Academic Community. Final Technical Report.

    ERIC Educational Resources Information Center

    Bayer, Bernard

    The Mechanized Information Center (MIC) at the Ohio State University conducts retrospective and current awareness searches for faculty, students, and staff using data bases for agriculture, chemistry, education, psychology, and social sciences, as well as a multidisciplinary data base. The final report includes (1) a description of the background…

  12. Project CREATE Final Report. Cooperative Resources To Enhance Access to Technology Education.

    ERIC Educational Resources Information Center

    Hampden County Employment and Training Consortium, Springfield, MA.

    These materials have been developed by Project CREATE (Cooperative Resources to Enhance Access to Jobs through Technical Education), a demonstration program designed to develop a network, specific activities, and resources that would provide education and support services to a wide audience. A 13-page final report describes the hands-on training…

  13. Digital-Computer Processing of Graphical Data. Final Report.

    ERIC Educational Resources Information Center

    Freeman, Herbert

    The final report of a two-year study concerned with the digital-computer processing of graphical data. Five separate investigations carried out under this study are described briefly, and a detailed bibliography, complete with abstracts, is included in which are listed the technical papers and reports published during the period of this program.…

  14. 48 CFR 1852.235-73 - Final Scientific and Technical Reports.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... regarding appropriate electronic formats for final reports is available at http://www.sti.nasa.gov under....nasa.gov under the “Get Help” link. (e) In accordance with paragraph (d) of the Rights in Data—General... contained in NPR 2200.2, Guidelines for Documentation, Approval, and Dissemination of NASA Scientific and...

  15. 48 CFR 1852.235-73 - Final Scientific and Technical Reports.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... regarding appropriate electronic formats for final reports is available at http://www.sti.nasa.gov under....nasa.gov under the “Get Help” link. (e) In accordance with paragraph (d) of the Rights in Data—General... contained in NPR 2200.2, Guidelines for Documentation, Approval, and Dissemination of NASA Scientific and...

  16. 48 CFR 1852.235-73 - Final Scientific and Technical Reports.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... regarding appropriate electronic formats for final reports is available at http://www.sti.nasa.gov under....nasa.gov under the “Get Help” link. (e) In accordance with paragraph (d) of the Rights in Data—General... contained in NPR 2200.2, Guidelines for Documentation, Approval, and Dissemination of NASA Scientific and...

  17. 48 CFR 1852.235-73 - Final Scientific and Technical Reports.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... regarding appropriate electronic formats for final reports is available at http://www.sti.nasa.gov under....nasa.gov under the “Get Help” link. (e) In accordance with paragraph (d) of the Rights in Data—General... contained in NPR 2200.2, Guidelines for Documentation, Approval, and Dissemination of NASA Scientific and...

  18. 10 CFR 52.79 - Contents of applications; technical information in final safety analysis report.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... assurance program will be implemented; (26) The applicant's organizational structure, allocations or... presents a safety analysis of the structures, systems, and components of the facility as a whole. The final... contain an analysis and evaluation of the major structures, systems, and components of the facility that...

  19. PFBC HGCU Test Facility. Technical progress report No. 24, Third quarter, CY 1995

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    This is the twenty-fourth and final Technical Progress Report submitted to the Department of Energy (DOE) in connection with the cooperative agreement between the DOE and Ohio Power Company for the Tidd PFBC Hot Gas Clean Up Test Facility. This report covers the work completed during the Third Quarter of CY 1995. All activity this quarter was directed toward the completion of the program final report. A draft copy of the final report was forwarded to DOE during this quarter, and DOE submitted their comments on the report to AEPSC. DOE requested that Westinghouse write an appendix to the reportmore » covering the performance of the fail-safe regenerator devices during Tad operation, and Westinghouse subsequently prepared the appendix. Additional DOE comments were incorporated into the report, and it will be issued in camera-ready form by the end of October, 1995, which is the program end date. Appendix 1 presents the results of filter candle posttest examination by Westinghouse performed on selected filter candles following final shutdown of the system.« less

  20. The missing graphical user interface for genomics.

    PubMed

    Schatz, Michael C

    2010-01-01

    The Galaxy package empowers regular users to perform rich DNA sequence analysis through a much-needed and user-friendly graphical web interface. See research article http://genomebiology.com/2010/11/8/R86 RESEARCH HIGHLIGHT: With the advent of affordable and high-throughput DNA sequencing, sequencing is becoming an essential component in nearly every genetics lab. These data are being generated to probe sequence variations, to understand transcribed, regulated or methylated DNA elements, and to explore a host of other biological features across the tree of life and across a range of environments and conditions. Given this deluge of data, novices and experts alike are facing the daunting challenge of trying to analyze the raw sequence data computationally. With so many tools available and so many assays to analyze, how can one be expected to stay current with the state of the art? How can one be expected to learn to use each tool and construct robust end-to-end analysis pipelines, all while ensuring that input formats, command-line options, sequence databases and program libraries are set correctly? Finally, once the analysis is complete, how does one ensure the results are reproducible and transparent for others to scrutinize and study?In an article published in Genome Biology, Jeremy Goecks, Anton Nekrutenko, James Taylor and the rest of the Galaxy Team (Goecks et al. 1) make a great advance towards resolving these critical questions with the latest update to their Galaxy Project. The ambitious goal of Galaxy is to empower regular users to carry out their own computational analysis without having to be an expert in computational biology or computer science. Galaxy adds a desperately needed graphical user interface to genomics research, making data analysis universally accessible in a web browser, and freeing users from the minutiae of archaic command-line parameters, data formats and scripting languages. Data inputs and computational steps are selected from dynamic graphical menus, and the results are displayed in intuitive plots and summaries that encourage interactive workflows and the exploration of hypotheses. The underlying data analysis tools can be almost any piece of software, written in any language, but all their complexity is neatly hidden inside of Galaxy, allowing users to focus on scientific rather than technical questions.

  1. Discovery of Genome-Wide Microsatellite Markers in Scombridae: A Pilot Study on Albacore Tuna

    PubMed Central

    Nikolic, Natacha; Duthoy, Stéphanie; Destombes, Antoine; Bodin, Nathalie; West, Wendy; Puech, Alexis; Bourjea, Jérôme

    2015-01-01

    Recent developments in sequencing technologies and bioinformatics analysis provide a greater amount of DNA sequencing reads at a low cost. Microsatellites are the markers of choice for a variety of population genetic studies, and high quality markers can be discovered in non-model organisms, such as tuna, with these recent developments. Here, we use a high-throughput method to isolate microsatellite markers in albacore tuna, Thunnus alalunga, based on coupling multiplex enrichment and next-generation sequencing on 454 GS-FLX Titanium pyrosequencing. The crucial minimum number of polymorphic markers to infer evolutionary and ecological processes for this species has been described for the first time. We provide 1670 microsatellite design primer pairs, and technical and molecular genetics selection resulting in 43 polymorphic microsatellite markers. On this panel, we characterized 34 random and selectively neutral markers («neutral») and 9 «non-neutral» markers. The variability of «neutral» markers was screened with 136 individuals of albacore tuna from southwest Indian Ocean (42), northwest Indian Ocean (31), South Africa (31), and southeast Atlantic Ocean (32). Power analysis demonstrated that the panel of genetic markers can be applied in diversity and population genetics studies. Global genetic diversity for albacore was high with a mean number of alleles at 16.94; observed heterozygosity 66% and expected heterozygosity 77%. The number of individuals was insufficient to provide accurate results on differentiation. Of the 9 «non-neutral» markers, 3 were linked to a sequence of known function. The one is located to a sequence having an immunity function (ThuAla-Tcell-01) and the other to a sequence having energy allocation function (ThuAla-Hki-01). These two markers were genotyped on the 136 individuals and presented different diversity levels. ThuAla-Tcell-01 has a high number of alleles (20), heterozygosity (87–90%), and assignment index. ThuAla-Hki-01 has a lower number of alleles (9), low heterozygosity (24–27%), low assignment index and significant inbreeding. Finally, the 34 «neutral» and 3 «non-neutral» microsatellites markers were tested on four economically important Scombridae species—Thunnus albacares, Thunnus thynnus, Thunnus obesus, and Acanthocybium solandri. PMID:26544051

  2. Median network analysis of defectively sequenced entire mitochondrial genomes from early and contemporary disease studies.

    PubMed

    Bandelt, Hans-Jürgen; Yao, Yong-Gang; Bravi, Claudio M; Salas, Antonio; Kivisild, Toomas

    2009-03-01

    Sequence analysis of the mitochondrial genome has become a routine method in the study of mitochondrial diseases. Quite often, the sequencing efforts in the search of pathogenic or disease-associated mutations are affected by technical and interpretive problems, caused by sample mix-up, contamination, biochemical problems, incomplete sequencing, misdocumentation and insufficient reference to previously published data. To assess data quality in case studies of mitochondrial diseases, it is recommended to compare any mtDNA sequence under consideration to their phylogenetically closest lineages available in the Web. The median network method has proven useful for visualizing potential problems with the data. We contrast some early reports of complete mtDNA sequences to more recent total mtDNA sequencing efforts in studies of various mitochondrial diseases. We conclude that the quality of complete mtDNA sequences generated in the medical field in the past few years is somewhat unsatisfactory and may even fall behind that of pioneer manual sequencing in the early nineties. Our study provides a paradigm for an a posteriori evaluation of sequence quality and for detection of potential problems with inferring a pathogenic status of a particular mutation.

  3. Genomic sequencing of Pleistocene cave bears

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Noonan, James P.; Hofreiter, Michael; Smith, Doug

    2005-04-01

    Despite the information content of genomic DNA, ancient DNA studies to date have largely been limited to amplification of mitochondrial DNA due to technical hurdles such as contamination and degradation of ancient DNAs. In this study, we describe two metagenomic libraries constructed using unamplified DNA extracted from the bones of two 40,000-year-old extinct cave bears. Analysis of {approx}1 Mb of sequence from each library showed that, despite significant microbial contamination, 5.8 percent and 1.1 percent of clones in the libraries contain cave bear inserts, yielding 26,861 bp of cave bear genome sequence. Alignment of this sequence to the dog genome,more » the closest sequenced genome to cave bear in terms of evolutionary distance, revealed roughly the expected ratio of cave bear exons, repeats and conserved noncoding sequences. Only 0.04 percent of all clones sequenced were derived from contamination with modern human DNA. Comparison of cave bear with orthologous sequences from several modern bear species revealed the evolutionary relationship of these lineages. Using the metagenomic approach described here, we have recovered substantial quantities of mammalian genomic sequence more than twice as old as any previously reported, establishing the feasibility of ancient DNA genomic sequencing programs.« less

  4. Genetic evolution of pancreatic cancer: lessons learnt from the pancreatic cancer genome sequencing project

    PubMed Central

    Iacobuzio-Donahue, Christine A

    2012-01-01

    Pancreatic cancer is a disease caused by the accumulation of genetic alterations in specific genes. Elucidation of the human genome sequence, in conjunction with technical advances in the ability to perform whole exome sequencing, have provided new insight into the mutational spectra characteristic of this lethal tumour type. Most recently, exomic sequencing has been used to clarify the clonal evolution of pancreatic cancer as well as provide time estimates of pancreatic carcinogenesis, indicating that a long window of opportunity may exist for early detection of this disease while in the curative stage. Moving forward, these mutational analyses indicate potential targets for personalised diagnostic and therapeutic intervention as well as the optimal timing for intervention based on the natural history of pancreatic carcinogenesis and progression. PMID:21749982

  5. Lessons for surgeons in the final moments of Air France Flight 447.

    PubMed

    Bhangu, Aneel; Bhangu, Sonia; Stevenson, James; Bowley, Douglas M

    2013-06-01

    All surgeons make mistakes, and learning from critical incidents may help improve performance. The present study aimed to highlight lessons for surgeons from analysis of the final moments of Air France Flight 447. All of the authors work in teams and situations where safety, technical performance, and non-technical skills are critical. This review was born out of discussions regarding the events of Flight 447; specifically, whether the airline disaster was relevant to their work, and whether they could learn anything from it. The study is based on review of the crash reports of Flight 447, which lost flight speed indication after formation of ice prevented air from entering flight speed indicators during a storm. Following a subsequent stall, the aircraft fell at a rate of >10,000 feet/min until it crashed into the Atlantic Ocean, killing 228 passengers and crew. There were errors in decision making, reasoning, communication, and teamwork. Such non-technical skills failures have been recognized previously and can be addressed by existing non-technical skills training. A reliance on autopilot meant that the pilots were unfamiliar with high-altitude flying when the autopilot is disengaged. They were unprepared for and affected by such a sudden and serious problem; an event called "surprise and startle" by the accident investigation. The absence of the senior pilot (who was on a scheduled break) in the critical final minutes slowed error recognition and recovery. Unintended consequences of modern safety strategies may be under-recognized and can lead to adverse events. Both simulation-based and non-simulation-based training should include "surprise and startle" events beyond the scenarios trainees might expect. Likewise, in the face of increasing reliance on modern technology, surgeons should ensure that they would be able to perform procedures in the absence of such technologies. Specific training may improve surgeons' non-technical skills, and recognition of such skills could also be used to help select future surgeons.

  6. Aircraft stress sequence development: A complex engineering process made simple

    NASA Technical Reports Server (NTRS)

    Schrader, K. H.; Butts, D. G.; Sparks, W. A.

    1994-01-01

    Development of stress sequences for critical aircraft structure requires flight measured usage data, known aircraft loads, and established relationships between aircraft flight loads and structural stresses. Resulting cycle-by-cycle stress sequences can be directly usable for crack growth analysis and coupon spectra tests. Often, an expert in loads and spectra development manipulates the usage data into a typical sequence of representative flight conditions for which loads and stresses are calculated. For a fighter/trainer type aircraft, this effort is repeated many times for each of the fatigue critical locations (FCL) resulting in expenditure of numerous engineering hours. The Aircraft Stress Sequence Computer Program (ACSTRSEQ), developed by Southwest Research Institute under contract to San Antonio Air Logistics Center, presents a unique approach for making complex technical computations in a simple, easy to use method. The program is written in Microsoft Visual Basic for the Microsoft Windows environment.

  7. Microbial Genomes Multiply

    NASA Technical Reports Server (NTRS)

    Doolittle, Russell F.

    2002-01-01

    The publication of the first complete sequence of a bacterial genome in 1995 was a signal event, underscored by the fact that the article has been cited more than 2,100 times during the intervening seven years. It was a marvelous technical achievement, made possible by automatic DNA-sequencing machines. The feat is the more impressive in that complete genome sequencing has now been adopted in many different laboratories around the world. Four years ago in these columns I examined the situation after a dozen microbial genomes had been completed. Now, with upwards of 60 microbial genome sequences determined and twice that many in progress, it seems reasonable to assess just what is being learned. Are new concepts emerging about how cells work? Have there been practical benefits in the fields of medicine and agriculture? Is it feasible to determine the genomic sequence of every bacterial species on Earth? The answers to these questions maybe Yes, Perhaps, and No, respectively.

  8. Modeling read counts for CNV detection in exome sequencing data.

    PubMed

    Love, Michael I; Myšičková, Alena; Sun, Ruping; Kalscheuer, Vera; Vingron, Martin; Haas, Stefan A

    2011-11-08

    Varying depth of high-throughput sequencing reads along a chromosome makes it possible to observe copy number variants (CNVs) in a sample relative to a reference. In exome and other targeted sequencing projects, technical factors increase variation in read depth while reducing the number of observed locations, adding difficulty to the problem of identifying CNVs. We present a hidden Markov model for detecting CNVs from raw read count data, using background read depth from a control set as well as other positional covariates such as GC-content. The model, exomeCopy, is applied to a large chromosome X exome sequencing project identifying a list of large unique CNVs. CNVs predicted by the model and experimentally validated are then recovered using a cross-platform control set from publicly available exome sequencing data. Simulations show high sensitivity for detecting heterozygous and homozygous CNVs, outperforming normalization and state-of-the-art segmentation methods.

  9. Sequencing-based diagnostics for pediatric genetic diseases: progress and potential

    PubMed Central

    Tayoun, Ahmad Abou; Krock, Bryan; Spinner, Nancy B.

    2016-01-01

    Introduction The last two decades have witnessed revolutionary changes in clinical diagnostics, fueled by the Human Genome Project and advances in high throughput, Next Generation Sequencing (NGS). We review the current state of sequencing-based pediatric diagnostics, associated challenges, and future prospects. Areas Covered We present an overview of genetic disease in children, review the technical aspects of Next Generation Sequencing and the strategies to make molecular diagnoses for children with genetic disease. We discuss the challenges of genomic sequencing including incomplete current knowledge of variants, lack of data about certain genomic regions, mosaicism, and the presence of regions with high homology. Expert Commentary NGS has been a transformative technology and the gap between the research and clinical communities has never been so narrow. Therapeutic interventions are emerging based on genomic findings and the applications of NGS are progressing to prenatal genetics, epigenomics and transcriptomics. PMID:27388938

  10. Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples

    PubMed Central

    Bushell, Claire A.; Grant, Paul R.; Cowen, Simon; Gutierrez-Aguirre, Ion; O'Sullivan, Denise M.; Žel, Jana; Milavec, Mojca; Foy, Carole A.; Nastouli, Eleni; Garson, Jeremy A.; Huggett, Jim F.

    2015-01-01

    Digital PCR (dPCR) is being increasingly used for the quantification of sequence variations, including single nucleotide polymorphisms (SNPs), due to its high accuracy and precision in comparison with techniques such as quantitative PCR (qPCR) and melt curve analysis. To develop and evaluate dPCR for SNP detection using DNA, RNA, and clinical samples, an influenza virus model of resistance to oseltamivir (Tamiflu) was used. First, this study was able to recognize and reduce off-target amplification in dPCR quantification, thereby enabling technical sensitivities down to 0.1% SNP abundance at a range of template concentrations, a 50-fold improvement on the qPCR assay used routinely in the clinic. Second, a method was developed for determining the false-positive rate (background) signal. Finally, comparison of dPCR with qPCR results on clinical samples demonstrated the potential impact dPCR could have on clinical research and patient management by earlier (trace) detection of rare drug-resistant sequence variants. Ultimately this could reduce the quantity of ineffective drugs taken and facilitate early switching to alternative medication when available. In the short term such methods could advance our understanding of microbial dynamics and therapeutic responses in a range of infectious diseases such as HIV, viral hepatitis, and tuberculosis. Furthermore, the findings presented here are directly relevant to other diagnostic areas, such as the detection of rare SNPs in malignancy, monitoring of graft rejection, and fetal screening. PMID:26659206

  11. Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples.

    PubMed

    Whale, Alexandra S; Bushell, Claire A; Grant, Paul R; Cowen, Simon; Gutierrez-Aguirre, Ion; O'Sullivan, Denise M; Žel, Jana; Milavec, Mojca; Foy, Carole A; Nastouli, Eleni; Garson, Jeremy A; Huggett, Jim F

    2016-02-01

    Digital PCR (dPCR) is being increasingly used for the quantification of sequence variations, including single nucleotide polymorphisms (SNPs), due to its high accuracy and precision in comparison with techniques such as quantitative PCR (qPCR) and melt curve analysis. To develop and evaluate dPCR for SNP detection using DNA, RNA, and clinical samples, an influenza virus model of resistance to oseltamivir (Tamiflu) was used. First, this study was able to recognize and reduce off-target amplification in dPCR quantification, thereby enabling technical sensitivities down to 0.1% SNP abundance at a range of template concentrations, a 50-fold improvement on the qPCR assay used routinely in the clinic. Second, a method was developed for determining the false-positive rate (background) signal. Finally, comparison of dPCR with qPCR results on clinical samples demonstrated the potential impact dPCR could have on clinical research and patient management by earlier (trace) detection of rare drug-resistant sequence variants. Ultimately this could reduce the quantity of ineffective drugs taken and facilitate early switching to alternative medication when available. In the short term such methods could advance our understanding of microbial dynamics and therapeutic responses in a range of infectious diseases such as HIV, viral hepatitis, and tuberculosis. Furthermore, the findings presented here are directly relevant to other diagnostic areas, such as the detection of rare SNPs in malignancy, monitoring of graft rejection, and fetal screening. Copyright © 2016 Whale et al.

  12. Ancient Biomolecules and Evolutionary Inference.

    PubMed

    Cappellini, Enrico; Prohaska, Ana; Racimo, Fernando; Welker, Frido; Pedersen, Mikkel Winther; Allentoft, Morten E; de Barros Damgaard, Peter; Gutenbrunner, Petra; Dunne, Julie; Hammann, Simon; Roffet-Salque, Mélanie; Ilardo, Melissa; Moreno-Mayar, J Víctor; Wang, Yucheng; Sikora, Martin; Vinner, Lasse; Cox, Jürgen; Evershed, Richard P; Willerslev, Eske

    2018-04-25

    Over the last decade, studies of ancient biomolecules-particularly ancient DNA, proteins, and lipids-have revolutionized our understanding of evolutionary history. Though initially fraught with many challenges, the field now stands on firm foundations. Researchers now successfully retrieve nucleotide and amino acid sequences, as well as lipid signatures, from progressively older samples, originating from geographic areas and depositional environments that, until recently, were regarded as hostile to long-term preservation of biomolecules. Sampling frequencies and the spatial and temporal scope of studies have also increased markedly, and with them the size and quality of the data sets generated. This progress has been made possible by continuous technical innovations in analytical methods, enhanced criteria for the selection of ancient samples, integrated experimental methods, and advanced computational approaches. Here, we discuss the history and current state of ancient biomolecule research, its applications to evolutionary inference, and future directions for this young and exciting field. Expected final online publication date for the Annual Review of Biochemistry Volume 87 is June 20, 2018. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

  13. Current and future resources for functional metagenomics

    PubMed Central

    Lam, Kathy N.; Cheng, Jiujun; Engel, Katja; Neufeld, Josh D.; Charles, Trevor C.

    2015-01-01

    Functional metagenomics is a powerful experimental approach for studying gene function, starting from the extracted DNA of mixed microbial populations. A functional approach relies on the construction and screening of metagenomic libraries—physical libraries that contain DNA cloned from environmental metagenomes. The information obtained from functional metagenomics can help in future annotations of gene function and serve as a complement to sequence-based metagenomics. In this Perspective, we begin by summarizing the technical challenges of constructing metagenomic libraries and emphasize their value as resources. We then discuss libraries constructed using the popular cloning vector, pCC1FOS, and highlight the strengths and shortcomings of this system, alongside possible strategies to maximize existing pCC1FOS-based libraries by screening in diverse hosts. Finally, we discuss the known bias of libraries constructed from human gut and marine water samples, present results that suggest bias may also occur for soil libraries, and consider factors that bias metagenomic libraries in general. We anticipate that discussion of current resources and limitations will advance tools and technologies for functional metagenomics research. PMID:26579102

  14. Current and future resources for functional metagenomics.

    PubMed

    Lam, Kathy N; Cheng, Jiujun; Engel, Katja; Neufeld, Josh D; Charles, Trevor C

    2015-01-01

    Functional metagenomics is a powerful experimental approach for studying gene function, starting from the extracted DNA of mixed microbial populations. A functional approach relies on the construction and screening of metagenomic libraries-physical libraries that contain DNA cloned from environmental metagenomes. The information obtained from functional metagenomics can help in future annotations of gene function and serve as a complement to sequence-based metagenomics. In this Perspective, we begin by summarizing the technical challenges of constructing metagenomic libraries and emphasize their value as resources. We then discuss libraries constructed using the popular cloning vector, pCC1FOS, and highlight the strengths and shortcomings of this system, alongside possible strategies to maximize existing pCC1FOS-based libraries by screening in diverse hosts. Finally, we discuss the known bias of libraries constructed from human gut and marine water samples, present results that suggest bias may also occur for soil libraries, and consider factors that bias metagenomic libraries in general. We anticipate that discussion of current resources and limitations will advance tools and technologies for functional metagenomics research.

  15. Plasma Physics Network Newsletter, No. 3

    NASA Astrophysics Data System (ADS)

    1991-02-01

    This issue of the Newsletter contains a report on the First South-North International Workshop on Fusion Theory, Tipaza, Algeria, 17-20 September, 1990; a report in the issuance of the 'Buenos Aires Memorandum' generated during the IV Latin American Workshop on Plasma Physics, Argentina, July 1990, and containing a proposal that the IFRC establish a 'Steering Committee on North-South Collaboration in Controlled Nuclear Fusion and Plasma Physics Research'; the announcement that the 14th International Conference on Plasma Physics and Controlled Nuclear Fusion will be held in Wuerzburg, Germany, September 30 to October 7, 1992; a list of IAEA technical committee meetings for 1991; an item on ITER news; an article 'Long Term Physics R and D Planning (for ITER)' by F. Engelmann; in the planned sequence of 'Reports on National Fusion Programs' contributions on the Chinese and Yugoslav programs; finally, the titles and contacts for two other newsletters of potential interest, i.e., the AAAPT (Asian African Association for Plasma Training) Newsletter, and the IPG (International physics Group-A sub unit of the American Physical Society) Newsletter.

  16. Applying cognitive load theory to the redesign of a conventional database systems course

    NASA Astrophysics Data System (ADS)

    Mason, Raina; Seton, Carolyn; Cooper, Graham

    2016-01-01

    Cognitive load theory (CLT) was used to redesign a Database Systems course for Information Technology students. The redesign was intended to address poor student performance and low satisfaction, and to provide a more relevant foundation in database design and use for subsequent studies and industry. The original course followed the conventional structure for a database course, covering database design first, then database development. Analysis showed the conventional course content was appropriate but the instructional materials used were too complex, especially for novice students. The redesign of instructional materials applied CLT to remove split attention and redundancy effects, to provide suitable worked examples and sub-goals, and included an extensive re-sequencing of content. The approach was primarily directed towards mid- to lower performing students and results showed a significant improvement for this cohort with the exam failure rate reducing by 34% after the redesign on identical final exams. Student satisfaction also increased and feedback from subsequent study was very positive. The application of CLT to the design of instructional materials is discussed for delivery of technical courses.

  17. Enhancement of the Probabilistic CEramic Matrix Composite ANalyzer (PCEMCAN) Computer Code

    NASA Technical Reports Server (NTRS)

    Shah, Ashwin

    2000-01-01

    This report represents a final technical report for Order No. C-78019-J entitled "Enhancement of the Probabilistic Ceramic Matrix Composite Analyzer (PCEMCAN) Computer Code." The scope of the enhancement relates to including the probabilistic evaluation of the D-Matrix terms in MAT2 and MAT9 material properties card (available in CEMCAN code) for the MSC/NASTRAN. Technical activities performed during the time period of June 1, 1999 through September 3, 1999 have been summarized, and the final version of the enhanced PCEMCAN code and revisions to the User's Manual is delivered along with. Discussions related to the performed activities were made to the NASA Project Manager during the performance period. The enhanced capabilities have been demonstrated using sample problems.

  18. [Methods, challenges and opportunities for big data analyses of microbiome].

    PubMed

    Sheng, Hua-Fang; Zhou, Hong-Wei

    2015-07-01

    Microbiome is a novel research field related with a variety of chronic inflamatory diseases. Technically, there are two major approaches to analysis of microbiome: metataxonome by sequencing the 16S rRNA variable tags, and metagenome by shot-gun sequencing of the total microbial (mainly bacterial) genome mixture. The 16S rRNA sequencing analyses pipeline includes sequence quality control, diversity analyses, taxonomy and statistics; metagenome analyses further includes gene annotation and functional analyses. With the development of the sequencing techniques, the cost of sequencing will decrease, and big data analyses will become the central task. Data standardization, accumulation, modeling and disease prediction are crucial for future exploit of these data. Meanwhile, the information property in these data, and the functional verification with culture-dependent and culture-independent experiments remain the focus in future research. Studies of human microbiome will bring a better understanding of the relations between the human body and the microbiome, especially in the context of disease diagnosis and therapy, which promise rich research opportunities.

  19. The anatomy of the gyroscope, part 3

    NASA Astrophysics Data System (ADS)

    Cousins, Frank W.; Hollington, John L.

    1990-03-01

    The gyroscope and its applications are collated with the technical information to be found in the patent literature, augmented by that found in textbooks and technical journels. A detailed consideration is given to early mechanical gyroscopes. Included is a discussion of gyroscopic gears, vibratory gyroscopes, interferometric gyroscopes, and the use of the gyroscope in optical and related devices and deals finally with the difficult problem of gyroscopic inertial drives.

  20. Query Storage and Relay in Research Root (LACREND RR)

    DTIC Science & Technology

    2017-05-30

    QUERY STORAGE AND RELAY IN RESEARCH ROOT (LACREND-RR) UNIVERSITY OF SOUTHERN CALIFORNIA MAY 2017 FINAL TECHNICAL REPORT APPROVED FOR PUBLIC ...exempt from public affairs security and policy review in accordance with SAF/AQR memorandum dated 10 Dec 08 and AFRL/CA policy clarification...memorandum dated 16 Jan 09. This report is available to the general public , including foreign nationals. Copies may be obtained from the Defense Technical

  1. The Chrysler-Weldtech Approach to Retraining Displaced Workers. A Model Project to Develop Specialized Welding Skills Required for Modern Auto Assembly Operations. Final Technical Report.

    ERIC Educational Resources Information Center

    Lyons, Thomas M.; Knight, Glen A.

    A model project was conducted to demonstrate how Chrysler, in partnership with the education community and the government, could provide technical training to enable displaced workers to contribute to the "H-Body" car launch, to improve their job skills, and to enhance their future employability. The training was conducted on a pilot…

  2. Heat pump assisted geothermal heating system for Felix Spa, Romania

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rosca, Marcel; Maghiar, Teodor

    1996-01-24

    The paper presents a pre-feasibility type study of a proposed heat pump assisted geothermal heating system for an average hotel in Felix Spa, Romania. After a brief presentation of the geothermal reservoir, the paper gives the methodology and the results of the technical and economical calculations. The technical and economical viability of the proposed system is discussed in detail in the final part of the paper.

  3. Heat pump assisted geothermal heating system for Felix Spa, Romania

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rosca, M.; Maghiar, T.

    1996-12-31

    The paper presents a pre-feasibility type study of a proposed heat pump assisted geothermal heating system for an average hotel in Felix Spa, Romania. After a brief presentation of the geothermal reservoir, the paper gives the methodology and the results of the technical and economical calculations. The technical and economical viability of the proposed system is discussed in detail in the final part of the paper.

  4. The 1990 Johnson Space Center bibliography of scientific and technical papers

    NASA Technical Reports Server (NTRS)

    1991-01-01

    Abstracts are presented of scientific and technical papers written and/or presented by L. B. Johnson Space Center (JSC) authors, including civil servants, contractors, and grantees, during the calendar year of 1990. Citations include conference and symposium presentations, papers published in proceedings or other collective works, seminars, and workshop results, NASA formal report series (including contractually required final reports), and articles published in professional journals.

  5. Solar Heating and Cooling of Buildings: Phase 0. Feasibility and Planning Study. Volume 1: Executive Summary. Document No. 74SD419. Final Report.

    ERIC Educational Resources Information Center

    General Electric Co., Philadelphia, PA. Space Div.

    The purpose of this study was to establish the technical and economic feasibility of using solar energy for the heating and cooling of buildings and to provide baseline information for the widespread application of solar energy. The initial step in this program was a study of the technical, economic, societal, legal, and environmental factors…

  6. Prototype development and demonstration for response, emergency staging, communications, uniform management, and evacuation (R.E.S.C.U.M.E.) : technical report on prototype development and field testing of R.E.S.C.U.M.E. applications.

    DOT National Transportation Integrated Search

    2015-04-01

    This document is the final project technical report for the development and field testing of the Response, Emergency Staging, Communications, Uniform Management, and Evacuation (R.E.S.C.U.M.E.) application bundle, with a focus on the Incident Zone (I...

  7. Review of Military Mountain Medicine Technology and Research Barriers

    DTIC Science & Technology

    2011-09-01

    NORTH ATLANTIC TREATY ORGANISATION RESEARCH AND TECHNOLOGY ORGANISATION AC/323(HFM-146)TP/387 www.rto.nato.int RTO TECHNICAL REPORT TR...montagne et les freins à la recherche) Final Report of Task Group HFM-146. Published September 2011 Distribution and Availability...323(HFM-146)TP/387 www.rto.nato.int RTO TECHNICAL REPORT TR-HFM-146 Review of Military Mountain Medicine Technology and Research Barriers (Point

  8. SELECTIVE DISSEMINATION OF INFORMATION--REVIEW OF SELECTED SYSTEMS AND A DESIGN FOR ARMY TECHNICAL LIBRARIES. FINAL REPORT. ARMY TECHNICAL LIBRARY IMPROVEMENT STUDIES (ATLIS), REPORT NO. 8.

    ERIC Educational Resources Information Center

    BIVONA, WILLIAM A.

    THIS REPORT PRESENTS AN ANALYSIS OF OVER EIGHTEEN SMALL, INTERMEDIATE, AND LARGE SCALE SYSTEMS FOR THE SELECTIVE DISSEMINATION OF INFORMATION (SDI). SYSTEMS ARE COMPARED AND ANALYZED WITH RESPECT TO DESIGN CRITERIA AND THE FOLLOWING NINE SYSTEM PARAMETERS--(1) INFORMATION INPUT, (2) METHODS OF INDEXING AND ABSTRACTING, (3) USER INTEREST PROFILE…

  9. Air Force Research Laboratory Wright Site Guide to Technical Publishing

    DTIC Science & Technology

    2005-04-01

    Scientific and Technical Reports—Elements, Organization, and Design manual (and a version modified for documents generated for AFRL) • Merriam-Webster’s...notice page --SF 298 --original graphics /halftones Indicate the following on the letter of transmittal sheet: --quantity of copies required for...Elements, Organization and Design ? The WRS CDRL for a final report requires that the standard be followed. The only exception is SBIR Phase 1

  10. The Effect of Drycleaning Moisture on Fused Cloth Systems

    DTIC Science & Technology

    1989-03-01

    TECHNICAL REPORT NATICK/TR-89/024 et, THE EFFECT OF DRYCLEANING MOISTURE ON FUSED CLOTH SYSTEMS BY ELIZABETH J. MORELAND International...MOISTUP.E ON FUSED CLOTH SYSTEMS 12. PERSONAL AUTMOR(S) Elizabeth J. MorelanJ 13«. TYPE OF REPORT Final Technical Report 13b. TIME COVERED...This project was initiated to investigate the effect of moisture in drycleaning systems on preselected fused cloth structures. Adverse surface

  11. Annual National Leadership Development Seminar for State Directors of Vocational Education (4th, Las Vegas, Nev., September 14-17, 1971). Comprehensive Personnel Development for Vocational-Technical Education. Final Report.

    ERIC Educational Resources Information Center

    Kazarian, Edward N., Ed.; Ward, Darrell, L., Ed.

    The proceedings of this seminar, attended by state directors and other leaders in vocational and technical education from 41 states, the District of Columbia, Saipan and Puerto Rico, contain presentations by 18 leaders from industry, government, and education. Specific objectives were to: (1) provide a forum for presentations concerning personnel…

  12. Leader Identity, Individual Differences, and Leader Self-Development

    DTIC Science & Technology

    2012-09-01

    and Social Sciences Approved for public release; distribution is unlimited. U.S. Army Research Institute for the Behavioral and Social ... Social Sciences, Attn: DAPC-ARI-MS, 6000 6th Street, Fort Belvoir, VA 22060-5586. FINAL DISPOSITION: Destroy this Technical Report when it is no...longer needed. Do not return it to the U.S. Army Research Institute for the Behavioral and Social Sciences. NOTE: The findings in this Technical

  13. Optimizing Operational Physical Fitness (Optimisation de L’Aptitude Physique Operationnelle)

    DTIC Science & Technology

    2009-01-01

    NORTH ATLANTIC TREATY ORGANISATION RESEARCH AND TECHNOLOGY ORGANISATION AC/323(HFM-080)TP/200 www.rto.nato.int RTO TECHNICAL REPORT TR... RESEARCH AND TECHNOLOGY ORGANISATION AC/323(HFM-080)TP/200 www.rto.nato.int RTO TECHNICAL REPORT TR-HFM-080 Optimizing Operational Physical...Fitness (Optimisation de l’aptitude physique opérationnelle) Final Report of Task Group 019. ii RTO-TR-HFM-080 The Research and

  14. Rating Training Continuum: Development Procedures

    DTIC Science & Technology

    1992-01-01

    During the workshop, the SMEs will need to review technical documents to refresh their memory and resolve any technical issues. It is important that a...endorsement. 7 additional memory space was very useful. The computers with the 80-megabyte hard disk drive were used to collect all final PPPs and TLAs...description, general, and documentation). If you reed to refresh your memory , refer to Figure 3-5 for the definitions associated with the indirectly

  15. Test Plan Development for Plastic Ammunition Containers. Volume 1

    DTIC Science & Technology

    1989-03-15

    1850 Black Canyon Stage¶I Packaging division (SMCAR-AEP) Picatinny Phoenix, Arizona 85027 Arsenal, New Jersey 07806-5000 86. NAME OF FUNDING...packaging containers. The report is presented in two separate volumes. Volume I contains the Final Technical Report and includes the analysis of... Division of the U.S. Army Armament Research, Development and Engineering Center. Mr. Jasper C. Griggs and Mr. D. E. Jones served as technical consultants

  16. The communicative functions of final rises in Finnish intonation.

    PubMed

    Ogden, Richard; Routarinne, Sara

    2005-01-01

    This paper considers the communicative function of final rises in Finnish conversational talk between pairs of teenage girls. Final rises are fairly common, occurring approximately twice a minute, predominantly on declaratives and in narrative sequences. We briefly consider the interplay between voice quality (known to be a marker of transition relevance) and rising intonation in Finnish. We argue that in narrative sequences, rising terminals manage two main interactional tasks: they provide a place for a coparticipant to mark recipiency, and they project more talk by the current speaker. Using a methodology which combines phonetic observation with conversation analysis, we demonstrate participants' orientation to these functions.

  17. Final technical report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Richman, M.

    1996-08-01

    Granular flows of nearly elastic, identical spheres down bumpy inclines are described. A numerical technique is developed to overcome the difficulties associated with the ill-defined `tops` of these flows.

  18. TEAM TRAINING. FINAL TECHNICAL REPORT FEBRUARY 1966-FEBRUARY 1967.

    ERIC Educational Resources Information Center

    BRIGGS, GEORGE E.; JOHNSTON, WILLIAM A.

    THIS IS THE FINAL REPORT ON A FOUR-YEAR PROGRAM OF LABORATORY RESEARCH ON TEAM TRAINING IN A COMBAT INFORMATION CENTER (CIC) CONTEXT. THE RESEARCH LITERATURE ON TEAM TRAINING IS REVIEWED, AND A SET OF CONCLUSIONS IS DRAWN WITH REGARD TO TEAM PERFORMANCE AS A FUNCTION OF TASK, TRAINING, AND COMMUNICATIONS VARIABLES. IN ADDITION, THE IMPLICATIONS…

  19. Development of Advanced Technologies to Reduce Design, Fabrication and Construction Costs for Future Nuclear Power Plants

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    DiNunzio, Camillo A.; Gupta, Abhinav; Golay, Michael

    2002-11-30

    This report presents a summation of the third and final year of a three-year investigation into methods and technologies for substantially reducing the capital costs and total schedule for future nuclear plants. In addition, this is the final technical report for the three-year period of studies.

  20. Validation of Technical Recommendations. Final Report. ISSOE Managing Student Progress.

    ERIC Educational Resources Information Center

    Ridley, Dennis; And Others

    This report is organized to provide a complete, logical presentation of the major steps taken in the third phase of research on dynamics of dissemination of the Instructional Support System for Occupational Education (ISSOE). It reports on the confirmation and validation of the Phase II Final Report on Dissemination Issues and the Phase III…

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